LOCUS       DAZFAH010000001        69749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFAH010000001 DAZFAH010000000
VERSION     DAZFAH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.001"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..202
                     /locus_tag="ACHJWB_000001"
     CDS             50..202
                     /locus_tag="ACHJWB_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311708.1"
                     /translation="MQWKIINGWYCVTACGFMSWKFRTLQEGIKWAFVSKEARDVAND
                     NEIWEG"
     gene            205..504
                     /locus_tag="ACHJWB_000002"
     CDS             205..504
                     /locus_tag="ACHJWB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311709.1"
                     /translation="MNVNQQKNLQKIMLTFDKDYRLSEQLYDRQVELIESIRLHQLSS
                     TFDVVTGKGVRHEVLEAAKDSPEFEELMDAYRREAMAIIARWDLADQLDGQRDAA"
     gene            501..1400
                     /locus_tag="ACHJWB_000003"
     CDS             501..1400
                     /locus_tag="ACHJWB_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFO4311710.1"
                     /translation="MMRNAGIMDRTKYIGGSDVAGILGISPWRTPLEVYLDKVQPRVK
                     PVDPSKQKVFTRGQRMEPYVIDLLSEETGMEIVHRGNRYIHRDYDFIAAEIDAEAASG
                     ENIEIKTVSPFKAKEWGEIQTDAIPVHYTAQAMHGLMVTNKQVCVFGVLIGGDDFRIY
                     RVERDEETIQAILEKEIAFWDRVKNLNPPEAASVSDVSLMFEKDAGTSIEADGKALAL
                     FNDLRDMKSRRKSLEEEIAISEEKLKMYMQEHSVLTLDGKPLCTWKSQISNRFDQKLF
                     QSVHPELFEKFKTTTTQRVFRMK"
     gene            1410..2432
                     /gene="recT"
                     /locus_tag="ACHJWB_000004"
     CDS             1410..2432
                     /gene="recT"
                     /locus_tag="ACHJWB_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO4311711.1"
                     /translation="MSINALKAAATGNQVAHHNEKPTTLAGLLADPKIKAQMALALPK
                     HMTADRLARIATTEIRKVPKLASCDQASFLGAIMQCAQLGLEPGGALGHAYLIPFDKR
                     QKVNGRWETVSTEAQLIIGYRGMIDLARRSGQILSISARTVHTNDKFSYSYGLEETLE
                     HLPCETGDRGELTHVYAVARLKDGGVQFEVMSRADIEKVRALSKAGSSGPWVDHFDEM
                     AKKTVIRRLFKYLPVSIEMQKAVVMDERAEAGLSQDNAAVITGEYSVVDDERQHLSPI
                     SDSEREEAREYIIAILNSLDPSAEDAKTMFKRAENEINTMAEKLGDEYHQKFMMTLND
                     MRPEFE"
     gene            2482..2730
                     /locus_tag="ACHJWB_000005"
     CDS             2482..2730
                     /locus_tag="ACHJWB_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4311712.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            2888..3139
                     /locus_tag="ACHJWB_000006"
     CDS             2888..3139
                     /locus_tag="ACHJWB_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4311713.1"
                     /translation="MRSPSKIVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            3132..3782
                     /locus_tag="ACHJWB_000007"
     CDS             3132..3782
                     /locus_tag="ACHJWB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO4311714.1"
                     /translation="MTKKYTLIYADPPWVYRDKAADGNRGASFKYPVMSVMDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIE
                     LFARQTSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDNQGRERAA"
     gene            3779..3973
                     /locus_tag="ACHJWB_000008"
     CDS             3779..3973
                     /locus_tag="ACHJWB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4311715.1"
                     /translation="MNRASPADLRKCLETANMLAHSGIRFVPIPAVTDAEFATLSAIF
                     ENKIESLAAEAEMEENQQNN"
     gene            complement(3977..5227)
                     /locus_tag="ACHJWB_000009"
     CDS             complement(3977..5227)
                     /locus_tag="ACHJWB_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4311716.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMAEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRSHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGGHIQGFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WINGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(5420..6997)
                     /gene="guaA"
                     /locus_tag="ACHJWB_000010"
     CDS             complement(5420..6997)
                     /gene="guaA"
                     /locus_tag="ACHJWB_000010"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4311717.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(7066..8532)
                     /gene="guaB"
                     /locus_tag="ACHJWB_000011"
     CDS             complement(7066..8532)
                     /gene="guaB"
                     /locus_tag="ACHJWB_000011"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4311718.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            8694..10064
                     /gene="xseA"
                     /locus_tag="ACHJWB_000012"
     CDS             8694..10064
                     /gene="xseA"
                     /locus_tag="ACHJWB_000012"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4311719.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(10061..10276)
                     /gene="yfgJ"
                     /locus_tag="ACHJWB_000013"
     CDS             complement(10061..10276)
                     /gene="yfgJ"
                     /locus_tag="ACHJWB_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4311720.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10345..11817)
                     /gene="der"
                     /locus_tag="ACHJWB_000014"
     CDS             complement(10345..11817)
                     /gene="der"
                     /locus_tag="ACHJWB_000014"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4311721.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEKEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(11935..13113)
                     /gene="bamB"
                     /locus_tag="ACHJWB_000015"
     CDS             complement(11935..13113)
                     /gene="bamB"
                     /locus_tag="ACHJWB_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4311722.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNTDDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(13124..13744)
                     /gene="yfgM"
                     /locus_tag="ACHJWB_000016"
     CDS             complement(13124..13744)
                     /gene="yfgM"
                     /locus_tag="ACHJWB_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4311723.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(13762..15036)
                     /gene="hisS"
                     /locus_tag="ACHJWB_000017"
     CDS             complement(13762..15036)
                     /gene="hisS"
                     /locus_tag="ACHJWB_000017"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4311724.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(15147..16265)
                     /gene="ispG"
                     /locus_tag="ACHJWB_000018"
                     /gene_synonym="gcpE"
     CDS             complement(15147..16265)
                     /gene="ispG"
                     /locus_tag="ACHJWB_000018"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4311725.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAVGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(16292..17305)
                     /gene="rodZ"
                     /locus_tag="ACHJWB_000019"
     CDS             complement(16292..17305)
                     /gene="rodZ"
                     /locus_tag="ACHJWB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4311726.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(17590..18744)
                     /gene="rlmN"
                     /locus_tag="ACHJWB_000020"
     CDS             complement(17590..18744)
                     /gene="rlmN"
                     /locus_tag="ACHJWB_000020"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4311727.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(18894..19325)
                     /gene="ndk"
                     /locus_tag="ACHJWB_000021"
     CDS             complement(18894..19325)
                     /gene="ndk"
                     /locus_tag="ACHJWB_000021"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4311728.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(19474..21786)
                     /gene="pbpC"
                     /locus_tag="ACHJWB_000022"
     CDS             complement(19474..21786)
                     /gene="pbpC"
                     /locus_tag="ACHJWB_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4311729.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEVAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(21787..26748)
                     /gene="yfhM"
                     /locus_tag="ACHJWB_000023"
     CDS             complement(21787..26748)
                     /gene="yfhM"
                     /locus_tag="ACHJWB_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4311730.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEDSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            26955..27800
                     /gene="sseA"
                     /locus_tag="ACHJWB_000024"
     CDS             26955..27800
                     /gene="sseA"
                     /locus_tag="ACHJWB_000024"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4311731.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTYPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            28136..28261
                     /gene="timP"
                     /locus_tag="ACHJWB_000025"
     CDS             28136..28261
                     /gene="timP"
                     /locus_tag="ACHJWB_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4311732.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(28293..29069)
                     /gene="sseB"
                     /locus_tag="ACHJWB_000026"
     CDS             complement(28293..29069)
                     /gene="sseB"
                     /locus_tag="ACHJWB_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4311733.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(29211..30494)
                     /gene="pepB"
                     /locus_tag="ACHJWB_000027"
     CDS             complement(29211..30494)
                     /gene="pepB"
                     /locus_tag="ACHJWB_000027"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4311734.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(30672..30872)
                     /gene="iscX"
                     /locus_tag="ACHJWB_000028"
     CDS             complement(30672..30872)
                     /gene="iscX"
                     /locus_tag="ACHJWB_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4311735.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(30884..31219)
                     /gene="fdx"
                     /locus_tag="ACHJWB_000029"
     CDS             complement(30884..31219)
                     /gene="fdx"
                     /locus_tag="ACHJWB_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4311736.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(31221..33071)
                     /gene="hscA"
                     /locus_tag="ACHJWB_000030"
     CDS             complement(31221..33071)
                     /gene="hscA"
                     /locus_tag="ACHJWB_000030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4311737.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSDAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(33088..33603)
                     /gene="hscB"
                     /locus_tag="ACHJWB_000031"
     CDS             complement(33088..33603)
                     /gene="hscB"
                     /locus_tag="ACHJWB_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4311738.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(33699..34022)
                     /gene="iscA"
                     /locus_tag="ACHJWB_000032"
     CDS             complement(33699..34022)
                     /gene="iscA"
                     /locus_tag="ACHJWB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4311739.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(34039..34425)
                     /gene="iscU"
                     /locus_tag="ACHJWB_000033"
     CDS             complement(34039..34425)
                     /gene="iscU"
                     /locus_tag="ACHJWB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4311740.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(34453..35667)
                     /gene="iscS"
                     /locus_tag="ACHJWB_000034"
     CDS             complement(34453..35667)
                     /gene="iscS"
                     /locus_tag="ACHJWB_000034"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4311741.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(35908..36396)
                     /gene="iscR"
                     /locus_tag="ACHJWB_000035"
     CDS             complement(35908..36396)
                     /gene="iscR"
                     /locus_tag="ACHJWB_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4311742.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(36666..37406)
                     /gene="trmJ"
                     /locus_tag="ACHJWB_000036"
     CDS             complement(36666..37406)
                     /gene="trmJ"
                     /locus_tag="ACHJWB_000036"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4311743.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37525..38328
                     /gene="suhB"
                     /locus_tag="ACHJWB_000037"
     CDS             37525..38328
                     /gene="suhB"
                     /locus_tag="ACHJWB_000037"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4311744.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            38473..39327
                     /gene="yfhR"
                     /locus_tag="ACHJWB_000038"
     CDS             38473..39327
                     /gene="yfhR"
                     /locus_tag="ACHJWB_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4311745.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            39518..40798
                     /gene="csiE"
                     /locus_tag="ACHJWB_000039"
     CDS             39518..40798
                     /gene="csiE"
                     /locus_tag="ACHJWB_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4311746.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(40790..41929)
                     /gene="hcaT"
                     /locus_tag="ACHJWB_000040"
     CDS             complement(40790..41929)
                     /gene="hcaT"
                     /locus_tag="ACHJWB_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4311747.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(42089..42979)
                     /gene="hcaR"
                     /locus_tag="ACHJWB_000041"
     CDS             complement(42089..42979)
                     /gene="hcaR"
                     /locus_tag="ACHJWB_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4311748.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            43115..44476
                     /gene="hcaE"
                     /locus_tag="ACHJWB_000042"
     CDS             43115..44476
                     /gene="hcaE"
                     /locus_tag="ACHJWB_000042"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4311749.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            44473..44991
                     /gene="hcaF"
                     /locus_tag="ACHJWB_000043"
     CDS             44473..44991
                     /gene="hcaF"
                     /locus_tag="ACHJWB_000043"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4311750.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            44991..45311
                     /gene="hcaC"
                     /locus_tag="ACHJWB_000044"
     CDS             44991..45311
                     /gene="hcaC"
                     /locus_tag="ACHJWB_000044"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4311751.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            45308..46120
                     /gene="hcaB"
                     /locus_tag="ACHJWB_000045"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             45308..46120
                     /gene="hcaB"
                     /locus_tag="ACHJWB_000045"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4311752.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            46130..47344
                     /gene="hcaD"
                     /locus_tag="ACHJWB_000046"
     CDS             46130..47344
                     /gene="hcaD"
                     /locus_tag="ACHJWB_000046"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4311753.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLPLPLLPPPWFWSDQYSDNLQF
                     IGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAK
                     LLIDENIALKSL"
     gene            47441..47863
                     /gene="yphA"
                     /locus_tag="ACHJWB_000047"
     CDS             47441..47863
                     /gene="yphA"
                     /locus_tag="ACHJWB_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4311754.1"
                     /translation="MNTLRYFDYGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(47911..48783)
                     /gene="yphB"
                     /locus_tag="ACHJWB_000048"
     CDS             complement(47911..48783)
                     /gene="yphB"
                     /locus_tag="ACHJWB_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4311755.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANQVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(48795..49889)
                     /gene="yphC"
                     /locus_tag="ACHJWB_000049"
     CDS             complement(48795..49889)
                     /gene="yphC"
                     /locus_tag="ACHJWB_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4311756.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(49922..50920)
                     /gene="yphD"
                     /locus_tag="ACHJWB_000050"
     CDS             complement(49922..50920)
                     /gene="yphD"
                     /locus_tag="ACHJWB_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4311757.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(50945..52456)
                     /gene="yphE"
                     /locus_tag="ACHJWB_000051"
     CDS             complement(50945..52456)
                     /gene="yphE"
                     /locus_tag="ACHJWB_000051"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4311758.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYASSQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(52479..53462)
                     /gene="yphF"
                     /locus_tag="ACHJWB_000052"
     CDS             complement(52479..53462)
                     /gene="yphF"
                     /locus_tag="ACHJWB_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4311759.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(53559..56840)
                     /gene="yphG"
                     /locus_tag="ACHJWB_000053"
     CDS             complement(53559..56840)
                     /gene="yphG"
                     /locus_tag="ACHJWB_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4311760.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDYQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            56958..58151
                     /gene="yphH"
                     /locus_tag="ACHJWB_000054"
     CDS             56958..58151
                     /gene="yphH"
                     /locus_tag="ACHJWB_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4311761.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(58215..59468)
                     /gene="glyA"
                     /locus_tag="ACHJWB_000055"
     CDS             complement(58215..59468)
                     /gene="glyA"
                     /locus_tag="ACHJWB_000055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4311762.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            59796..60986
                     /gene="hmpA"
                     /locus_tag="ACHJWB_000056"
     CDS             59796..60986
                     /gene="hmpA"
                     /locus_tag="ACHJWB_000056"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4311763.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(61031..61369)
                     /gene="glnB"
                     /locus_tag="ACHJWB_000057"
     CDS             complement(61031..61369)
                     /gene="glnB"
                     /locus_tag="ACHJWB_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4311764.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(61430..62764)
                     /gene="glrR"
                     /locus_tag="ACHJWB_000058"
     CDS             complement(61430..62764)
                     /gene="glrR"
                     /locus_tag="ACHJWB_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4311765.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(62754..63467)
                     /gene="qseG"
                     /locus_tag="ACHJWB_000059"
     CDS             complement(62754..63467)
                     /gene="qseG"
                     /locus_tag="ACHJWB_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4311766.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(63632..65059)
                     /gene="qseE"
                     /locus_tag="ACHJWB_000060"
     CDS             complement(63632..65059)
                     /gene="qseE"
                     /locus_tag="ACHJWB_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4311767.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(65635..69522)
                     /gene="purL"
                     /locus_tag="ACHJWB_000061"
                     /gene_synonym="purI"
     CDS             complement(65635..69522)
                     /gene="purL"
                     /locus_tag="ACHJWB_000061"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4311768.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAVLFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        16598 a        19155 c        17589 g        16407 t
ORIGIN      
        1 aaataagcat aattattacc tcaacggtaa cagaccgagg taacaagtta tgcagtggaa
       61 aatcatcaac ggttggtact gcgttactgc atgcggattc atgagctgga agttccgcac
      121 cttacaggaa ggcattaagt gggctttcgt cagcaaagaa gctcgcgatg tagccaacga
      181 taacgagata tgggagggct gataatgaac gttaatcagc agaaaaatct tcaaaaaatc
      241 atgctgacat tcgacaagga ctaccgcctg tcagaacagc tatatgaccg acaagttgaa
      301 ctgattgaga gcatccgact tcatcaactg tcctcaactt tcgacgttgt aacaggcaaa
      361 ggcgttcgtc atgaagtgct ggaggctgct aaagacagcc ctgagttcga agaactgatg
      421 gatgcctacc ggcgcgaggc aatggcaatt atagcgcgct gggatctggc ggatcagctt
      481 gatgggcaga gggacgcggc atgatgcgga acgctggaat catggataga acaaaataca
      541 tcggaggaag cgatgttgca gggattcttg gaattagccc atggcgcacc ccgcttgagg
      601 tttatctgga taaggtccag ccacgtgtca aaccagtaga cccaagcaag cagaaagttt
      661 tcacgcgtgg ccagcgtatg gagccatacg taatagacct gctttctgag gaaacaggga
      721 tggaaatcgt tcatcgcgga aaccgctata tccaccgtga ttacgatttt attgcagctg
      781 agatcgatgc agaagcagcg tcaggcgaga acattgagat caaaacagtt agtccgttca
      841 aagccaaaga atggggagaa atccagacag atgcaattcc tgtgcattac acggcccagg
      901 ccatgcacgg gttgatggtt acaaacaaac aggtatgcgt tttcggtgtg cttatcggtg
      961 gcgacgactt ccgaatctat cgggttgagc gtgatgaaga aactatccag gcgatcttag
     1021 aaaaagaaat cgctttctgg gaccgagtga aaaatcttaa cccgccggaa gctgccagcg
     1081 taagcgatgt atcgctgatg tttgagaaag atgccgggac aagtatcgag gctgacggaa
     1141 aggcactcgc actattcaac gatctacgag acatgaaatc acgcagaaaa tcactggaag
     1201 aagaaatagc tatatcagaa gagaagctga agatgtacat gcaagagcac tcagtcctga
     1261 ccctggacgg aaagccgctc tgcacatgga aatctcagat cagcaacaga ttcgaccaga
     1321 agctattcca gtcagtacac cctgagttat tcgaaaaatt caaaacaaca acgacacaac
     1381 gcgtcttcag aatgaagtaa ggagaaaaaa tgtctatcaa tgcacttaag gcagcggcta
     1441 ccggtaacca agttgcacat cataatgaga aaccaacaac tctggccgga cttctggcag
     1501 acccaaaaat taaagctcag atggctttgg cacttccaaa gcacatgaca gcagaccgtc
     1561 tggcgcgcat agcaaccaca gagatccgaa aggttccaaa acttgcatca tgcgaccaag
     1621 ccagcttcct gggggcaatt atgcaatgtg cccaattggg tcttgaacca ggcggagctc
     1681 ttggacacgc ttacctgata ccgttcgaca aacgccagaa agtaaatgga agatgggaaa
     1741 ccgtatctac agaagcacag ctgattatcg gctatcgcgg aatgattgac cttgcccgcc
     1801 gctctgggca gatcctgagt atctcggctc gtaccgtaca tacaaacgac aaattcagct
     1861 actcatacgg cctggaagaa acgctcgagc atttaccttg cgaaacaggt gaccgcggag
     1921 aattaacgca cgtttacgcc gttgcacgac tgaaagatgg cggagtccaa tttgaagtta
     1981 tgagccgggc agacattgag aaagttcgtg cactgagcaa agccggtagc agtggcccat
     2041 gggttgatca cttcgatgag atggctaaaa aaacagtaat tcgccgacta ttcaaatatc
     2101 ttcctgtttc tattgaaatg cagaaggctg ttgttatgga tgagcgcgct gaagctggac
     2161 ttagccaaga taacgcagct gttatcactg gtgaatattc cgtagttgac gatgagcgtc
     2221 aacacctgtc gccaatttca gattcagaac gagaagaagc tcgagaatat atcatcgcga
     2281 tacttaatag cctggatcca tctgctgaag atgcaaaaac gatgttcaag cgcgctgaaa
     2341 atgagattaa caccatggct gaaaagctcg gtgatgaata tcaccaaaaa ttcatgatga
     2401 cgcttaacga tatgcgtcca gaattcgagt aaccaccacc gcggcgccac acgcgccgca
     2461 ctgcaaccaa gagaggtatt tatgaaaggt gcattaggta agaaggaact cctggcggtg
     2521 gtgccactgt catggagcac tatcgaccgt atggagcgcg caggggaatt tcctaaacgc
     2581 tggtatatca ctgacaaacg ctgcgcatgg aaccgtgacg aagttgagcg ttggcttgat
     2641 gaacgtcagg cagcaagccc ggcagagttc cagggtaaaa aacctcctgt tcagcaacgt
     2701 gtatatcgtc ccgtgagcaa cgctgcatga gtgcgctgct aaggcactgg atcaaatggt
     2761 caggatggta cttattcctg gcctctgttt cagcatggct ttatctgctg gcattaattt
     2821 tcagagaggg ttggattaag tgagaaagtt aagccgactt gaaaaatatc acatgaataa
     2881 ggtttcaatg cgcagtccgt caaagattgt cgccgttact cctgcggcga tagagatcga
     2941 aaaacgcgcg attgaaagag agaaaaaagg gcagttccgc attgccgctc acctttggct
     3001 tcagtgtatg gatgttgctt ctggtgatgt tgagcgtgca aggatcgcgg ttcgcaggga
     3061 ccaatgtatc acaaaaggta acggccttcg ccgtggagac tatagcggta taggatgttg
     3121 cggggtggtt tatgactaag aaatacacac taatctatgc agatccaccc tgggtatacc
     3181 gggacaaagc cgcagatggt aatcgcggtg ccagttttaa atatccggtt atgagtgtga
     3241 tggatatctg ccgccttcct gtgtgggatt tggccgatga aaactgtctg ttggccatgt
     3301 ggtgggtgcc aacacaacca ctcgaagcgc taaaagttgt tgaagcctgg ggattccgtc
     3361 tgatgaccat gaagggcttc acgtggataa aatgtggtag tcgacaacca gataaactgg
     3421 ttatgggtat gggacacatg actcgcgcca atagtgaaga ttgcctgttt gcggtaaagg
     3481 gaaaactacc tacgcgcatt aatgcaggga tcgttcagtc atttaccgca ccgcggcttg
     3541 agcattcaag aaagccagat atcgttcgtg aaaaacttgt gcaattatta ggcgatgttt
     3601 ctcgcattga actgttcgcc cgccagacgt ctcatggctt cgatgtttgg ggtaatcagt
     3661 gcgaagaccc ggcagtgcaa ctacaccctg gatacgcgtt ggatattggc ggattaacaa
     3721 atgcattcag caatgctccg ctgtcaccaa cagacaacca ggggcgggag cgtgctgcat
     3781 gaacagggca tcaccagcag atttaagaaa atgccttgaa actgcaaaca tgcttgcaca
     3841 cagcgggatc aggtttgttc caattcccgc tgtcactgat gctgaatttg caacactgtc
     3901 agcaatattc gaaaacaaaa ttgaatcact ggcagcagaa gccgagatgg aagaaaatca
     3961 gcagaataat taaacgttat tcccccgcca tccacttctc aaacttcgac ggggagaacg
     4021 gaatcagatc cgtatgctcc ccgttaatcc aggaatcaat catatcggcc cactgctgca
     4081 acatgtaggc gcgctgtctg gcgtattccg ctttgttata tacggcgcgc acacctttct
     4141 gctcatgtgc cagagccttt tcaatccagt ctgaaggata accagcctca tgcaacaacg
     4201 tactggctgt acggcgcata tcgtgtacgg tgaagccctg aatatgccca ccatcttcat
     4261 ttattatttt caccgttctg tcgatcagag agttcagcgc ggcattagat aatggcttcc
     4321 ggaaattgta acgaccagga accagatatt cacttccacc agcgcacatc tgcaacccaa
     4381 ccaatatatc ctgtgcctgt ttaggcaggt aaataacgtg cgaccggctt cccttcatgc
     4441 ggtctgaagg aattgtccat gtccattttt taaaatctat ttcatcccac gttgcattgg
     4501 tgaattcgcc tttacgaacc atagtgataa gcaccagctt taaagccatt ttcatagttc
     4561 ccatagcacc aatggcatcc agcgtgcgga agaacaggcc aatttcttct ggtgtcagtg
     4621 ttcgctctcg tggtttaaat atggcgatag acgaaggttt aatgtcagcc gcaggattaa
     4681 acaaaccatg accacggtca ttggcgtgac ggtatacgct gctgatgatc tccctggcct
     4741 gtactgctgt tgcccgacca ccgcgttcga caatccggtc acacaaatca cgaaccatcg
     4801 atgtggtaat ttcagccatc attttgttgc caagaaccgg aagtatgtca cggtcgatca
     4861 ccgcctgctt cattgcgcgg gtactgtcag ccaggataac gtgtttcata taactgtcgg
     4921 tatgtaccgc aaacgtctcg gcaccacgaa tctttttgat accgtcacgt ttagccgcag
     4981 ccggtgactg gcctgcttta agcagcttct ttgcagcaat cagttcttcc cgcgcttctg
     5041 ccaggctgat accgtcacgc ccatactgcc cgattaccag tgtttcgcgg cgaccgttga
     5101 tacggtagtc atagcgaaac gagaccgtgc ctgacgtaag cacagctaca tacagcccgt
     5161 cacgatcgga gaccttgtac agtttgtcct gcggcttgag gttttttaat tttgtatcgg
     5221 taagcacaat tcacccgtat agaaaccatt ttcatgacgg tatgagagta tacctttaag
     5281 gtaataccgt cacctgtacc gccgaaaaat atggtgtaga gtgaatagaa atgaatacat
     5341 aaaaacaaaa accctctgta aaaacagagg gttgaattag tatctgaata ggaatgactt
     5401 gctatgagtt agctgttaat cattcccact caatggtagc tggcggcttg ccgctgatgt
     5461 catacaccac gcgggaaata ccgttcactt cattgataat gcggttggaa acgcgaccga
     5521 ggaaatcgta cggcagatgc gcccagtgtg cggtcataaa gtcgatggtt tcgacagcac
     5581 gcagagagac aacccagtca tacttacgac catcgcccat tacgccaacg gaacgtactg
     5641 gcaggaagac ggtgaacgcc tggctgactt tgtcgtacag gtccgcttta cgcagttctt
     5701 caatgaagat ggcgtcagca cggcgcagca ggtcacagta ctctttcttc acttcaccca
     5761 gaacacgaac gccaaggcct ggtcccggga acggatgacg gtacagcatg tcgtacggca
     5821 ggcccagctc cagaccaatc ttacgcactt cgtctttgaa cagctctttc agcggttcaa
     5881 ccaggcccat cttcatctct ttcggcaggc cgcccacgtt gtggtgagat ttgatgacgt
     5941 gtgctttacc ggttgcagaa gccgcagatt cgataacgtc agggtagatg gtgccctgcg
     6001 ccagccactt cacgtcttcc agtttcagcg cttcttcatc gaatacttca acgaaaacgc
     6061 gcccgatgat tttacgtttt gcttccggat cgttttcgcc agccagcgct gacaggaagc
     6121 gatcttctgc cggtacgtga acaatgttca gaccaaagtg atcgccaaac atatccagaa
     6181 cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac gaatacgcaa gtcaggtttt
     6241 taccgatagc gcggtgcagc agcattgcgg ttacggagga atccacacca ccagagaggc
     6301 cgaggatgac tttatcgtcg cctacctgct cgcggatgcg agctacagca tcgtcgataa
     6361 ttttcgctgg cgtccacagg gcttcacact ggcagatatc acgcacaaaa cgctccagca
     6421 tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa ctgtacgcca tagaagcgtt
     6481 tttcttcgtt agccataatg gcaaacgggc agctttcggt gctggctacg gtgacgaagt
     6541 cggacgggat agcggtaact ttatcgccgt ggctcatcca gacatcgagc agcggtttac
     6601 cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc gctgtcgttt acgacttcaa
     6661 cctgcgcgta gccaaattca cgttcgttag aggcttcaac gtgaccgccc aactgcattg
     6721 ccatggtctg catgccatag caaacgccga ataccggtac gcctgcttca aagacatact
     6781 gcggcgcacg cgggctgttt tcttcggtgg tgctttccgg gccgccggaa agaataatgc
     6841 cgcttggatt gaagtcacga atttgtgctt ctgtcacatc ccacgcccac agttcgcagt
     6901 aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt gtactgagaa ccgaagtcca
     6961 gaatgaggat gcgatgctta tgaatgtttt ccgtcattga cgcttattcc gaggcaagtg
     7021 aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa gagaatcagg agcccagacg
     7081 gtagttcggg gactctttag taatggtcac gtcgtgaacg tggctttcct gaatgcccgc
     7141 accgctgata cgtacaaact ccgctttagt acgcagttcg tcgatagtac cacagccggt
     7201 cagacccata caggagcgca ggccgcccat ctgctggtga atgatctctt tcaggcgacc
     7261 tttataagct acgcgacctt cgataccttc cggcaccagt ttgtcggcag cgttatcgct
     7321 ctggaaataa cggtcagagg aacctttgga catcgcgccc agggaaccca taccacggta
     7381 agatttgtaa gaacggccct ggtagagttc gatttcaccc ggagattctt cagtacccgc
     7441 cagcatggaa cctaccatca ccgcgcttgc gccagcggcg atagctttgg cgatgtcgcc
     7501 ggagaagcga ataccaccat ctgcgataac cggaataccg gtgccttcca gggcttcaac
     7561 tgcgtcagca acggcagtga tctgcggaac acctacgcca gtaacgatac gagtcgtaca
     7621 gatagaacca gggccgatac cgactttaac cgcgctgcaa ccagcttctg ccaaagcgcg
     7681 tgcacctgca gctgttgcca cgttgccgcc gatgatttgc agatccggat atttagcacg
     7741 ggtttcacgg atacgctgca aaacgccttc tgagtgaccg tgggaggagt cgatcagcag
     7801 aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct tcgttacccg cacctgcgcc
     7861 aactgcagca ccaacacgca gacggccttg ctcgtcttta caggcgttcg gtttacgttc
     7921 cgctttctgg aagtctttca cggtgatcat gccgatcagg tggaattcgt catcaaccac
     7981 cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc accacttcac gggcttcacc
     8041 ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa acgctaaccg gctggttcag
     8101 gtcggtaaca aaacgcacgt cacgaccggt gataataccc accagttcgt tttcttcggt
     8161 aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt tctttcactt cgcgcagcgt
     8221 cgtggttggc agaacagtct gcggatcagt caccacacca gattcgtgtt ttttcacacg
     8281 gcgaacttct tctgcctggc gttcaatgga catgtttttg tggataaagc cgataccgcc
     8341 ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg gtatccattg ctgcggaaag
     8401 catagggata ttcagacgaa tagttttcgt cagctgggtg ctgaggtcag cagtattcgg
     8461 cagaacggta gaatgagcag gaacgaggag aacgtcgtca aacgtcagag cttctttagc
     8521 gatacgtagc atgggcaata tctcgaccag agtggttaat aaatattgcc gcggcattat
     8581 acagagcgta accgattgca tctacccctt tttgcaaaaa atgcttgcta tccccgaagg
     8641 gcgggttact atcgactgaa taacctgctg atttagaatt tgatctcgct cacatgttac
     8701 cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa tcaaacggtt cgtctgctgc
     8761 ttgagcatga gatgggacag gtttggatca gcggcgaaat ctctaatttc acacaaccgg
     8821 cttccggtca ctggtacttt acactcaaag acgacaccgc ccaggtacgc tgcgcgatgt
     8881 tccgcaacag caaccgccgg gtgaccttcc gtccacagca tggacaacaa gttttagttc
     8941 gcgccaatat tacgctctac gagccgcgcg gtgactacca gataatcgtt gagagtatgc
     9001 agccggccgg tgaagggctg ctgcaacaga agtacgaaca gctcaaagcg aagttgcagg
     9061 ctgaaggttt gttcgatcag caatacaaaa aaccacttcc ctcccctgcg cattgcgttg
     9121 gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat tttgcatgtg ttaaaacgtc
     9181 gcgatccttc tctaccggtg atcatctacc ccaccgccgt tcagggcgat gacgcgccgg
     9241 ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa tgagtgcgac gtgttgatcg
     9301 ttgggcgcgg cggcggttcg ctggaagatt tatggagttt taacgacgaa cgcgtagcgc
     9361 gggctatttt tgccagccgc attccggtcg tcagcgccgt cgggcatgag acggatgtga
     9421 ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc atctgccgcc gctgaagtag
     9481 taagccgtaa tcagcaagag ttactgcgcc aggtgcaatc ggcccgtcaa cggctggaga
     9541 tggcgatgga ttattatctc gccaaccgta cgcgtcgctt tacgcagatt catcatcgct
     9601 tgcagcagca gcatccgcag ctccggctgg cacgccagca aaccatgctt gaacgcctgc
     9661 aaaaacggat gagctttgcg ctggaaaatc agcttaagcg tgccgggcaa cagcagcagc
     9721 gattaacaca gcggctgaat cagcaaaatc cacagccgaa gattcatcgc acgcaaacgc
     9781 gcattcagca actggaatat cgtttagcag aaaccctgcg cgtacagctt agcgccacgc
     9841 gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt aagcccactg tcaacgctgg
     9901 cgcgtggata cagcgttact actgctactg acggcaatgt actgaaaaaa gtgaagcaag
     9961 ttaaagcggg tgaaatgcta accacacgtc tggaagacgg ctggatagaa agtgaagtta
    10021 aaaacatcca gccggtaaaa aaatcgcgta aaaaagtgca ttaagccagc acgaattcaa
    10081 cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa gtaatctact gcaccgcagg
    10141 cttttaacac ctgcaacggt tgatggcagt ctggacaaag agctttcatt tcaataattt
    10201 ccccgcagga tgggcaacgc gcatggtcgt tatcctgatc aagcacatgc tgacattgtg
    10261 gacaatgcag ttccatatga attcctccgg tagccatccc tctacattca tagagggaat
    10321 ggcagataaa atacttacgg ataattattt atttttcttg atgtgcttca tcagacgctt
    10381 acgtttacgc atctgggttg gcgtcagggt gttacgcttg ttcgcatacg ggttttcccc
    10441 ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc agcgatttgc ggaagtagtt
    10501 catcaagtag cgcttgtagg aatcaggcag gtctttcacc tgattaccgt gaatcaccac
    10561 aatcggaggg ttataaccac cagcgtgggc atatttcagc ttcacacgac gaccgcgtac
    10621 cagcggcggt tggtgatctt caacagccat cgtcatgatg cgcgtcagca tagaggtccc
    10681 cacacgacgg gtggagctgt catacgcttc acgtactgat tcaaacaagt taccaacacc
    10741 actgccgtgc aaggcagaga taaagtgcac acgagcaaaa tcgataaagc ccagacggaa
    10801 gtccagcgtt tctttcacct gctctttcac ttcctggctc aggccatccc acttattcac
    10861 cacaatgaca agtgagcgcc cactattgag aataaagccc agcagcgaga gatcctgatc
    10921 ggaaatacct tcgcgcgcat caatcactaa catcaccacg ttggcgtctt caatggcctg
    10981 caacgttttg attacggaga atttctctac agcatcggtg attttgccgc gtttacgtac
    11041 gccagcggtg tcaatgagca catactcacg tccatcgcgt tccattggga tgtagatgct
    11101 gtcacgcgtc gtgccaggca tgtcataaac aacaacgcgc tcttcaccaa gaatacggtt
    11161 agtgagtgta gacttaccta cgttcggacg acccacaatc gccagtttga tcggcagact
    11221 ttgcgggtcg aagtcgtctt cctctttttc ttcgccgttc tcttccgctt caaattgcgc
    11281 ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg agatcttcca tccacggcag
    11341 cagcacatgc tccagcagac ttaatacgcc acgaccgtga gacgcggcga tcgggtagat
    11401 ttcacctaag ccaagcgagt agaaatcaac cactgcctga tcgggatcca gaccgtcagt
    11461 tttgtttgcc accaggaagg tcggtttttc acgggagcgc agatgtttgg caatcgcttc
    11521 atctgccggc atcaagcccg cgcgcgcatc caccataaac agtacgacgt ccgcttcttc
    11581 aatcgccagc agcgactgtt ccgccatgcg ggtttctaca ccgtcttctg tgccatcaat
    11641 cccgccggta tcaatacaga taaactcacg gccttcaatt tccgcacgac cgtacttacg
    11701 gtcacgagtc agacccggga aatccgcaac cagcgcatct cgggtgcgag ttagacggtt
    11761 aaataacgtg gattttccta cgttagggcg cccgacaagc gcgaccacag gtaccatgtt
    11821 taaagcctca tttttataaa tcatcagaca acgcacgcta tattcgcgtc gttgttaaaa
    11881 acaggaaaac ggcccctgtc caggagccgt tttcaaagtg aacgacagag acgattaacg
    11941 tgtaatagag tacacggttc cgtcttttgc ctggatcagc agtttgccgt cagcggcaac
    12001 cggttcagtc tggaaaccgg aactatcaac tttttgctgg gcaacgaaac gaccatcttc
    12061 gacgttaatc cagtgcagat aaccttcact gtcaccgacc accaggttgc cattatacag
    12121 caccggagaa gtcagcaggc gatgcaacag atcgctttgt gtccacagcg taacgccgcc
    12181 atcaatggtc aacgccatca cccggtcatt ttgatcgacc agatagatgc gattgccgtc
    12241 gacgatgaaa tcattcaccg aacccagttc gcgtttccac ataatctgac cactgcgcag
    12301 atcaagcgcc gtcaggttac cattataggc cagcgcgaaa acaacgccgt taacaacgac
    12361 gggagtcgtg tcaacatcgc tcagacggtc aatttcggta gaaccggtcg cctgggaaat
    12421 acgctgctgc caaatcatct ggccctgttc catcagcact gcgctgacgc gaccattatc
    12481 gccacccacg acggccgcac caaaagccgt tgccggcgca gactcgccac gcaaagagag
    12541 cgaaggcata tcgaggttaa ctgtccattt gacagcgccg tcagcttcgt tcagcgcttg
    12601 taactgaccg ttactggtgt ggattaacac cagaccgtcg ctgaccaccg ggcgcgaaag
    12661 tgcttcaccc gcgactttag tttgccatgc cacagtacca tcgctggtat tcagcgcgta
    12721 aacctgcgcc ttttcgctgc caatgtagac atgcccacca gacacggtca cgccgccaga
    12781 aagtaatgca ggctctttag agaaccagcc atctttctcg gccaggctga cagaccagat
    12841 ttctttgcca tcatccgtat tcagcgcttt taccaaacca gcgcggtccg ctgcatagac
    12901 aacgttgtcc gccagtgccg gatgaagatt ggaatagaag ttgccaatgc cgctaccaac
    12961 ggaagtactc cacgccgtgg tcggcgtaaa ctggttttca acggttggca atggggacat
    13021 ctttaccaca tcttcttcgc tgttaaacag cgaacagccg cttaaaaggg taacggaaag
    13081 cagtcctggc agcagtaatt tacgcaattg catcgggtcc ctctcagatg gacaaattat
    13141 taattttcat ctgcatcatt tcgctcagtg ccggagtaac atcgcttttc acgcctgctt
    13201 cccatgcact acgcgcacct tgcttatcac ctttgctcag caatgcttca ccacgcaggt
    13261 cggcaacaat ggcagcccac ccttcacctt tgatggtatc aagggttttc agcgcggcat
    13321 cagcctgctt gagctgtacc tgaacgcgag caagacgcag atttatcacg gctttgagat
    13381 tttcatcgct cgtgtctgcc agcccctgtt gtaactgggc ggcagctttc tctagttcat
    13441 ttttgtcaac aaattgctgc gcaagttcca aagaagccag cgcaccataa gtatttttat
    13501 tttcagcagc aaatttttcc gccgccggga tgctatccgg tttgccttcg ctcactgcgg
    13561 taaccgcatt ttgataggca agagaagcgg agcgtgcaga atcaacctga tggctgttcc
    13621 agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac cccaacagcc agtgctttgc
    13681 cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc gttttcgttc tcgtaaattt
    13741 ccacgctgtc cttctccttc cttaacccag taacgtgcgc aaatgcgcgg ctacgctatc
    13801 ctgcgcaact gccgtttgct caccagagcg caaatccttc actactgctg tgccgttagc
    13861 cacttcagac tcacccagca ccacagcaac gcgggcaccc catttatcag cacgggcaaa
    13921 ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat ttcacgcccg gtaattcatc
    13981 acgcagacgc tcagctaatg ccatagccgc agattgtgta tcagcacctg aagccaccag
    14041 gtatatatcg acaacaggat cggctttaaa ttccggatta acggcctgta ctaacaatac
    14101 aagacgttcg aggcccatcg caaaaccgac agccggtgtt gcacgaccgc ccagttgttc
    14161 cacaagaccg tcataacgac cgcctgcaca cacggtgccc tgggagccga gactgttagt
    14221 cacccactcg aaaacggtac ggttatagta atccagacca cgcaccagac gctggtttac
    14281 ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga ccggcaaaat gctcacgaga
    14341 ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg agaagcgcct gcacttccgg
    14401 attttttgaa tccagcacgc gcagcgggtt agtgtacatg cggcgtttgc agtcttcgtc
    14461 cagcttttct ttatgctgct caaggaatgc caccagcgca tcgcggtaat tggcgcgtgc
    14521 ttccagcgaa ccgatagaat tcagctcaag agttacgtgt tcggaaatac ccagcgcgcg
    14581 ccaccagcgg gcggtgagca taatcagttc agcgtcgata tccggacctt gcagaccgaa
    14641 aacttcgcag cccaactgat ggaactgacg ataacgccct ttctgcggac gctcgtgacg
    14701 gaacatcggc ccgatatacc acagacgctg ttcctgattg tacagaagac catgctcaat
    14761 gccggcgcgt acacagcccg ccgtcccttc agggcgcaga gtcaggctgt cgccattgcg
    14821 atcctcaaag gtgtacatct ctttttcaac cacgtcggtg acttcaccaa tcgcacgttt
    14881 gaatagcggg gtctgctcta caatcggcaa gcggatttca ctgtaaccgt agctgccgag
    14941 cacgtttttc agtgtgcctt caatgcgctg ccagatggcc gtttcgccag gcaggtaatc
    15001 gttcatgccg cgaatggctt gaatgttttt tgccacgttt attctctttc tgaatataaa
    15061 aatgaaccct caacgcttcc ctcaatgttt ggggagccat gcgggttcaa tcatacacgg
    15121 gaagcgaggc gcttcccatc acgttattat ttttcaacct gctgaacgtc aattcgacgc
    15181 gcttcatcca gctgactggc tttcgcacga atgcgtgctt ccagctggtc gatcatatcg
    15241 ttgttgtcca gacggtcttt gcgcacgcca tcttcataga ggccgctttt cttgttgccg
    15301 ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc cattcaccac gcagccgata
    15361 atcgaaacgt ccatcggagt gatgatatct tccaggcgtt gctccagcgc gttaaccgta
    15421 ccgataacat caaattcctg acgcgaacag gtcgggcagg cgatgaagtt aatccctcgc
    15481 gaacggatac gcagcgattt caaaatatcg aaaccgactt tgatctcttc gaccggatcg
    15541 gcagccagcg atacgcgcag cgtgtcgccg atgccttcag acagcagcag tcctaaacca
    15601 acggcggatt ttactgcccc gctgcgtgca ccacccgctt cggtgatccc cagatgcagc
    15661 ggctgatcga tctgttttgc cagcaaacga taagactcaa cagcgaggaa gacgtcagac
    15721 gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat cgagatgatc aacatgacgc
    15781 atggcggatt ccagcaatgc ctgcggcgtc ggttcgccat acttttcttg cagatctttt
    15841 tccagcgatc cggcgttaac gccaatacgg atcggaatgt ttttatcgcg cgcacagtca
    15901 accaccatgc gaatacgctc ttcattaccg atattgccag ggttaatacg cagacaatcg
    15961 acgccgtatt ccgctacttt cagcgcaatg cgatagtcaa agtggatgtc agccaccagc
    16021 ggcacgttaa cccgctgttt gatgagtttg aacgcttctg ccgcgtccat cgtcggtacg
    16081 gagacacgga cgatatcagc gccaacgcgt tccagcgcct tgatttgatt gaccgttgct
    16141 tcgacatctg tcgtacgcgt attggtcatg gactgtacgg cgatgggagc accatcgcca
    16201 atcggcacat tcccaacgta aatacgtgtt gattttctac gttgaattgg agcctggtta
    16261 tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc cggtgattgt tcggcattga
    16321 gggtcagacg cgcaacctgg ttagttctga taaaacgact cagatcgaca ggtttccctt
    16381 gatactggat ctgtactgcg gctggcgcac caattttcag tttgtacggt gcctggccgg
    16441 ttaagttcaa attaccgtct ttacgctgca taccgctaaa caatttttta ccggtagcat
    16501 cagtgacctc cagccagcaa tcggcagtaa agttcatcac cagcgcattc ggatcagcca
    16561 ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc agcaccatct ggcgttgttg
    16621 ctgccgttgg tgccggggtc gcggcggtat caacatttgc ctgcgaaggc gaaacaaccg
    16681 cattctgttg cggatcaaca gctggtgctg gcgcagttac ggcaggtgtt tgcgtgttgg
    16741 ttgcggtagt atccacagac gctggcggcg tggacgttgt agccgggtct gtagttgtcg
    16801 acgtatttaa cggaacactc tgcccctgct cgctattact gctcagttcc gccgaagatt
    16861 gatcggccat agtggtgatc tcttcctgct gagctttgtg gtcttgccac caccaggcac
    16921 cgctcaggcc gataaccaca aacaacacca gccaggtgaa ggtcatcagc cagccgtcac
    16981 gttttttgcg gcgtttaccg agggaaaaac tctgcatcgg cgcaactttc gcagcccgta
    17041 gtggagcctg cttttccagc cctggcagca gttcttcttc tggaatatgt accagacgcg
    17101 cataagagcg gatatatccg cgcaggaatg ttgaagcaag atcggcgggt gccttatctt
    17161 cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg ctcggcaacg gcctgctgac
    17221 taagtcctag ttgttcgcga gcattacgca ggcgagcgcc ggtagtaagt gcttcatttt
    17281 ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt ttaaatgaga attgagatgc
    17341 cggtgaacca tcatgctcac ccacaccgcg aaacatccgt taagttaacc gttatcatac
    17401 agtataagac cgtctgtgtg ctcgtgacaa agcccacatt cactaaggct aacttactgt
    17461 tgcatcgtta catactgcct taaagtcagc aaaaacgcac cgttaatatt gaccagacaa
    17521 ttgcaactta atacttcatt cataaaagat tacgccacgg tacataaagt aaccgtggcg
    17581 taatggctat cagaccgctt taatgtcgat ggcttcaccc tgcatccgtt tacgcagggt
    17641 acgtttcgta cggtcgataa catcgcccgc caactgacca caggcagcat cgatatcatc
    17701 accacgagtt ttacgcacaa tagtggtgaa accgtagctc atcagcactt ttgagaaacg
    17761 gtcgatacgg ctgttcgaac tgcgtccata cggcgcgccc gggaacgggt tccacgggat
    17821 caggttgatc ttacacggcg tatctttcag cagctccgcc agttggtgcg cgtgttcagt
    17881 gccgtcgtta acgtggtcaa gcatcacgta ttcaatagtg actcggccct gattggcgtt
    17941 ggatttctcc agataacggc gcaccgcagc aaggaacgtt tcgatattgt actttttgtt
    18001 gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg tgcagggaaa ttgccagtgc
    18061 aacgtcgatc atatcgccca gtttatccag cgccggaact acaccggaag tagaaagcgt
    18121 gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc atgatttcca tcgccggaac
    18181 gacgttgttc aggttgagca gcggctcgcc catgcccatc atcactacgt tagtgattgg
    18241 gcgctgaccg gtgacttttg ctgcgccgac gattttcgcc gcacgccaca cctggccgat
    18301 aatttccgac acccgcaggt tgcggttaaa gccctgctgg gcggtggaac agaatttaca
    18361 ctccagcgca caccccacct gcgaagagac gcagagcgtg gcacggtcgt cttccgggat
    18421 atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc catttaatgg tgccgtcgga
    18481 tgaacgctgt tcttcaacca cttccggtgc gcggatttcc gccacctctt tcagtttgcc
    18541 gcgcaacact ttgttgatgt cggtcatctc atcaaagttg tcgcagcaat agtgatacat
    18601 ccacttcatc acctgatcgg cgcggaaggg tttttcacct aaatctttaa aaaactcccg
    18661 catctgctga cggttgagat ccagcaggtt gatttttcca tctttcgtgg tgacgttttc
    18721 aggtgtgact aattgttcag acatatgcta ttccggcctc gttattacac gttatggccc
    18781 ctggagggtt gaaaaaagaa acgccccggt gagcggctgc tcgtccgggg gcgctgcatt
    18841 gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc atttacgaaa ttattaacgg
    18901 gtgcgcgggc acacttcgcc ttcgccaaag aaataagcga tttcgcgagc ggcagattcg
    18961 acggaatcag aaccgtgggt accgttttcg gtcaggctgt cagcgtaatc agcgcgcaga
    19021 gtaccagcca gtgcgtttgc cggattggtc gcgcccagca gatcgcggtg acgctgaacg
    19081 gcgttttcac cttccagcac ggaaaccacg atcgggccag aggtcatgaa ttcaaccaga
    19141 ccatcaaaga acggttttcc atcgtgttca gcataaaagc cacgtgcctg ttcaacggtc
    19201 aggtgcagca ttttggtgcc aacaattttg aaccctgcag cttcaaagcg cgcaaagata
    19261 ttaccaatga cgttttttgc taccgcgttc ggtttgatga tggaaaaagt acgttcaata
    19321 gccattttta cctctgtaaa ttgttctgtt gttgtctgta ccagcgtacg agatggcgcg
    19381 gattataatg agcaacaggg ccgttgacta ttgatgaagg taacattttt ttaaaataaa
    19441 atgagtttta gcaacaatca gggacaaaac agactattgc atgacaaatt tcactgtcgc
    19501 gatttgcccc acatcatcca tcaccagcaa ttgataatca cctttatccg tcaaatgcag
    19561 ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc agaaaccacc agcgttcacc
    19621 tgcccctcca cttgattgca acggcaaagt cgcttccgct gcgcccggta aacgtttaat
    19681 aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc tgggcatcgt gaccgtatgg
    19741 cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga cgttctgatg cgggcagcca
    19801 gggttccagc ggtagcggcc agacatttat catttcttgt cgcgcttgcg ggcaatcagc
    19861 ggcaacacgt ttgccatttt catccagcca gatggggaaa cgaatgccat taatgccttc
    19921 ctgctccggc agtaatagag ttggcggctg actcccgtcc agcagccagg ttgccaggcg
    19981 gcggcgacag ttaccgtcac cttccggcaa agactgtccg cccggccagc agataacgcc
    20041 acgagtgact gagttcgggc gcgggtcttc cggcagattc gcactgcgcg acagtaagat
    20101 attattgacc tgattcaaca atggtacggc actggcaaag ccaaactgac caacaacggg
    20161 cgtgccgtcc ggtctgccag tccagatccc aatgacatag cgagcgttaa ccccaatcgc
    20221 ccaggcgtca cgatagccat agctggtgcc cgttttccat gccagtgggg cgacgcgcgg
    20281 caaggcacta tccggcaagg gtttcgcttc atcagccata atccggcgaa tgatccacgc
    20341 cgcccccgac gacattaaag gccgttcaag cagcggatcg tcaggctgta agcgcaattt
    20401 gcctgccttg ccgtggcgag caaacgcggt atacgctgcc gccatatctt ccagttttgc
    20461 accagcgccg ccgagaatga gtgaaagatt cggcgcagca ccgttgggca aatataacgg
    20521 caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt ccataggctt ccagcacctg
    20581 cacagcaggt aagttcagcg agcgcaccag cgcctcgctc atgctgatcg ggccatgaaa
    20641 accgctatca aagttacctg gtcgataatc accggtgcgc cgggggacgt cttgcagcag
    20701 tgatgccggg tggatcaagc cttcatccag cgccagacca taaacaaacg gtttgagcac
    20761 cgatcctggc gatcggatcg cattgaccat atcaacatga ccaaagcgtg aatcatcgtt
    20821 gagatcaacc gatcccaccc agccgcgaac acgcatatcg gtatgatcaa ccacgatcat
    20881 cgccagtgag ctgcgcggtg gcaaccgccc tttccagttt tgcgccagtt cttccagacg
    20941 tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg cttttgcttt taccgagcat
    21001 catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg ggtgccagcc agatgggttc
    21061 ttcccttgac tcttttacct gctcacggga ccacacacct tgcacggcca tccgttcgag
    21121 cactttatta cgcgcggctt cggcacgctc cggccaacga tccgggcgaa gacggctggg
    21181 cgcttgcggc aaaaccgcca gcattgccac ctcggaatag cttaaattcg caggcgactt
    21241 tccgagataa gcccaacttg ccgcaccgat cccctgcaac gtaccgccaa acggagcgcg
    21301 gttaagatac aaggtcagaa tttcacgctt agacagatgc cattccagtt gcaacgcgcg
    21361 ccagagctgg cgaattttgc cgccaaatgt tttggggtga ggatcaagca gacgagcaac
    21421 ctgcatagtg agcgtgctgc caccggaaat aacccgtccc gaagtgagat cttgccatgc
    21481 tgcgcgcgcc accgagaatg gattcacccc cggatgcttc cagaaccagc gatcttcata
    21541 attgatcagc gcttcaaggt aacgtggaga aacatcttcg attgttaccg gataacgcca
    21601 gatgccgtca gcatcggcga agcgccatag cggcgtacca tcctgcgcca cgaccactcg
    21661 tgcgggattg acttcatgca gcggtagagg ccagagttta tctgctcccc acgctgcaag
    21721 aaaaaggaga aatggcgcag ctgccagcgt tatccagcag ccgcgtttgg ttaacaagcg
    21781 aggcatttac ggtcggacaa tcagcagatc ttcagccgcg ccggtcgccc gccattgggg
    21841 aacatacatt gattccacca tcggttgcgg tacctgatac gttcccggcg tcaccgcccg
    21901 cgccagatac accagcgtta ccggttggta ttcatcaacg gcaaccgccg ccacaaagcg
    21961 atcgtcacgg aactcaatgt gcttaatact cgcctgctgc atctggttca gtaagttctg
    22021 cacttcgcca ccactttgct ccaggctggc gctaccgttc gccagattct ggttttccag
    22081 ttccagaccc gcaggcagca gatccacgac caacgcatcc ggcacgctgt tactggcttt
    22141 tacctgcaac cacaccagca ccagatcgcc gctacgtaac gagtccagcg atttgctctt
    22201 accatcagta ccaagaatat gacgctcgat ttgcagcaca ttgttcgccg gtaaaggtgc
    22261 ggattgcgga taaccgctgg catccacacg cagccataac ggctgatcgc cactgtttct
    22321 cacctgcaag gtggcaagtt gatcgctatt cagattgctg tttagcgttt tctcgcctgt
    22381 cagcggctca gctgagaaag aggtttgcgc ctgccattta cccggtaaat cctgaatcgt
    22441 acgggcagcc aggaacaacg cgttactttc ctgcgtcgat agccagcgtt caccaaatgc
    22501 ctgctgcgaa agcgtgttca gcaaagtgtt ctgctcatcg ggtagcagtt tattttcttc
    22561 cagcaaggag agcattaacg cgttgtcgcg cagtggacta ccgtaatcac ccagccatat
    22621 ccgctcatca ctatttctcg gcgttttcag cgccagcaca atcgcttctt caccacgcgt
    22681 tgcatcaccc atggttttca gcgcaacgcc aagttgcagc agcggtaaac cagaagcggc
    22741 atctgcacga tgctcccaga tttcacgcag cgcacccagt ggtgcttttt gctgacgggc
    22801 cagcaccagc gcagcgtaag actgaacggc gaatttactg gctttgagat tatccgcgta
    22861 cgggatcgac atcatgcccg gatcttgtaa atagcgcagc agacgctcat taccccggtt
    22921 aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg cggaccagga aatccatcac
    22981 gtaagccgtc agccagtact cttcgtcacc gtttttatcc cacagcgcaa agccgccgtt
    23041 atcacgttgc atttgcagca aacgggaaat gccgatatcg accgatgcac ggcgtttctc
    23101 atcactgtcg cctttgatgc ccaacgcctg cagttgggct gcgttggtat aaagtgacgg
    23161 gaacaggccg ctggcggttt gctcaagaca gccgtacgga tacgctttta actctttgat
    23221 ataacgtgca atgttcagcg gtggtttgcc gctcaacaac aattgccctt ccagcgtaac
    23281 aggcgagaag ttttgcaatc catccgccgg aatcgcccat gtctcaccag gctgtaacgc
    23341 cgtaccgtaa ttaaccgttt gtgccgggaa cgccggacgg acgccgattt tccactgctt
    23401 atgctgatcg gcaacggttt cacccggtaa cgctaacccg ctaatggtgg cctgaatttc
    23461 tccatcgcca taacccggca atgctcgcac cgggataaac agcgtagtac gcacacctgg
    23521 cgctaattca acggctgcgg gtgaatcgct gaccagttca agcaaaccac tggcggtcag
    23581 ggcaacgttc agtttttgcg gtttatcggt aagattagtg atatccagcg tcagacgcga
    23641 ggtatcgcca ctcgccataa agcgcggcat gttcagttca gcaatcaccg gtgcggcaac
    23701 tatcactttg ctttcgttgc taccgaaatc atctgccgtc caggcttgcg ccatgacgcg
    23761 caattcaccg ttaaaatcgc caatcggcag tgtaaccgag tcttcgccct gttcgttgag
    23821 cgttaccggc agcgcctgct gcgcgacaat attgacgtga ttgaccggcg gtttaccacc
    23881 acgtttcagc tcatcaccat cgccaccgaa acgcagagct gccagacgcc cctgaccttc
    23941 aataacctga ccgtaaatat cgtaaatgtc tgcgccatag cgtttctgac caaagaacgc
    24001 ctgccacgga tctggcgtga cgtagtcagt aatattcagc acaccactat caacggcaga
    24061 caccagcaca ttcacctgtt taggcttctc gccattttta gtgctggctt taattttcac
    24121 ggttaatggt tgattgggac gcatttttgc tggtgtttcc agcgccagat cgaggcgacg
    24181 gttttcatcg ccaagcggca gatgcaacac accaaccgcg cgttttggcg tcgcggagcg
    24241 agatttatcg ccaggacgta ccaccagcgt acttaaatac agatcatgac gattccaggt
    24301 tttatcgacc ggaatcgtca gatccagccc ttgagccgga acatcaatct cttgccacca
    24361 cagcggccct tcactggact cgaccatcgc ataaccttta cccgccgttg gcgcggcgat
    24421 atgcaactta atggtgtcgc cagggcgata actggcttta tccagtttca gcgtgacacg
    24481 gtcgggtcgc actgcgccgc taccgtcgct gttgtcctgc cagctatagc cagcccagaa
    24541 acgaacacta ctgaccgctt cattcggcgc tttgacttcc agacgataag cgccccactc
    24601 taccggaaaa ctgaccttgc cggtttcgtc cgctttcaga tccagagttt gttcattttc
    24661 gatcagatct ttttgatcaa actgtgactg ccagccttca tcttctgacc agttccagta
    24721 gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc gacacggctt ttttcacgcc
    24781 ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg ccttcatcaa caatgggctg
    24841 tttcaccgtg ctgtcagtac gataatcgta aaccgatttc gaggcgaact gcggacggat
    24901 ccccggcaat gcatcggcag gccagatagc ctgctcagcg cggcgcgtca ccgggcgacc
    24961 gcccgattcc agcaaactac cctggaaaat aacctgtaat ggggaatgcg tttccttcca
    25021 ctggctttct gtagaaactt cgccgcgccc tttatcatcc agcgtcaact gaacttcatc
    25081 cagcgtgcgg gaaagatttt cggcagctat atcgccgaat tcaaaaccag gtaaggctga
    25141 cacagcttca cgcagtgggc gcaggaaaag ttgcccttgc aaagtattac cattagcagg
    25201 tgcaccatac aggtagtacc ccaccacgga gaatttcact tcatctttcg gcgttagcgg
    25261 ggttttctca ccggtcagat tcagcgccat gcgctctggc ataaaatctt cgacgtggaa
    25321 atcccacatc cgatactggt tatcgcccgt gttagcgcga atatgccaca taccggttgc
    25381 cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca ttctccggct gactaacgac
    25441 gctcctgagt acctgcccat cgggtttaat cacgtctaac ttgatgggtt gattgggcaa
    25501 cgctttaccg tctgcatcac gcagcaaacc attgaggatt accgtttcac ctgggcgata
    25561 aagatcgcgt gggccaaaca tgaaaaactg tttgctatag cctggcgcgc cagcaatgtt
    25621 aaattctgct aagtccagcg ccggaagttt taaatcgagt agcgttgtct gaccgtcttt
    25681 acgcgccaac agtaatgccg cgtttttatc attttccagc tgcacatgcc cctgagcgtc
    25741 actggttgcc tgagtcagag tctgcccttt ctcatttaat aaagagactt caattccttg
    25801 ctgggccgcg ccgttttcca gactttgggt aaagatatcc agacgattgt gataacggtg
    25861 agctgaaacg ccgatatcac ttaacgtaaa cagcgtcgcg ggattactgt aatcgtaacg
    25921 tccagcctga ttcatcacag ccagatacac gcccgcctgc tgaagcggtt tgatatcgcc
    25981 cagcggcagc aataattttt cacgggtgtt acgcgcagga ttgagatcaa accgtccggt
    26041 gtagaccaga tccgccatct gcagcagttt gtctgactgc cagttcgcca gcgaattgcg
    26101 gtattcccat tggctaatga atgctggcag agattctggc ttaacgcgga agaagttaac
    26161 atcgacatta ttaacgttga gcgccattac cggcagccct tcaacgactt tgccaggcag
    26221 cagcgaacca cggctggcaa aaccgacgct gggttggatg tcgcgggtag ttatagtttt
    26281 ttcgtaatct ttactgaagg ttgcgttgtt gagtgctttg acctccttgc caatagtaac
    26341 gatcaaatca cgtttcggtt cgaggtggcg taaacgtaac tctttaagat tatctgacag
    26401 ctcccaggca ccatccactt tgccgctttt tttatcgacg acatgaataa cgcgtgagaa
    26461 atcctgatcc gggtcgagag ggatggagaa cgtcagcacc agcgtggctg caccatcaag
    26521 ttggacttca gagagatcca gcaatgtcag caccttaccg gcactctgtt gggccagttt
    26581 ttgtctctcc ggcacggaga gttttgctga actcgcgttt tctgaagagg ccgctttagt
    26641 aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg ttatcgttgt tgtcgcaccc
    26701 tgccagcgcc agcattagca tgcaggcggc tacgcgtaac tttttcattt ttcatccctg
    26761 ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg tgagaattta cgttatgtaa
    26821 ttcagtatca cgcccataca atccgaattt tagtgagaat tgtggctcag acagccaaat
    26881 cagcctcttt cgcttgtcgc ttttcactaa acatccgaca atttagccta accccggcaa
    26941 aaatggagat gcctatgtcc acgacatggt ttgtaggagc cgactggctc gccgaacata
    27001 ttgatgaccc ggaaattcag attattgatg cccgcatggc gtcgcctgga caggaagatc
    27061 gtaacgttgc tcaggagtat ctgaatggac atattcccgg cgcagtgttt tttgatatcg
    27121 aagcgctttc tgatcacact tacccgcttc cgcacatgct gccgcgcccg gaaacgttcg
    27181 ccgtggcgat gcgtgaatta ggcgttaacc aggataagca cctgattgtc tatgacgaag
    27241 gtaatctttt ctcagcccca cgagcatggt ggatgctgcg cacctttggt gtagagaaag
    27301 tgtcgattct ggggggtgga cttgcaggct ggcagcgcga tgatctgctg ttagaagaag
    27361 gtgcagtaga gctgccggaa ggagagttta acgccgcgtt taatcctgaa gccgtggtga
    27421 aagtaaccga tgtattattg gcaagccatg aaaatacggc gcaaattatt gatgcccgcc
    27481 cggctgcacg ttttaacgca gaagttgatg aacctcgccc aggtttacgt cgcggacata
    27541 ttcccggagc actgaatgtt ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga
    27601 ccgatgaact ggatgcgata ttttttggtc gcggcgtcag ctacgacaaa ccaattatcg
    27661 tcagctgcgg ctctggtgta acggcagccg tggttttgtt agcactcgcg acgctggatg
    27721 tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac
    27781 cggttgagcc agtgaaataa gtattttaca ggcaataaaa aaccgccgaa tttggcggtt
    27841 ttttattgct ggtctggttc gcggcctttc cagctggttg tattaccgta gtaatgcaag
    27901 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
    27961 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
    28021 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
    28081 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
    28141 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
    28201 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
    28261 agcccgaatg ccgccctttg gggcggcata ttttagatta tccgattctg tttaaagtca
    28321 cgcaaaaaac caccccagcg acgttcatag aatggcacaa tatgttcggt aataaagtgg
    28381 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
    28441 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
    28501 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
    28561 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
    28621 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
    28681 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
    28741 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
    28801 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
    28861 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
    28921 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
    28981 agtagagtac ggaaaaaggc cgggcggtgc gccggttcag ttgctgcttt ttccagcagg
    29041 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
    29101 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt tttgaatttg
    29161 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
    29221 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
    29281 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
    29341 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
    29401 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaactc cgccagcggc
    29461 agacgccaga acggttcgtt ctcctgcgcg gcactcgcca gcaagcgacc ggccagcgca
    29521 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
    29581 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
    29641 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
    29701 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
    29761 aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg caaatgccag cgccccggta
    29821 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
    29881 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
    29941 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
    30001 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
    30061 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
    30121 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
    30181 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
    30241 cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc agcagcgatc cgcatcccag
    30301 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg cgccgcacgc
    30361 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
    30421 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
    30481 atcgcttctg tcattttagt tatccttctt tgtaaaaggg ccgccagttg gcagccccag
    30541 gatttttccg ctaaatgatt cgcgttgcag gaaggcggca agtgagtgaa gccccaggag
    30601 catagataac tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca
    30661 tgacgcggaa attattcggc ctcgtccagc cagactaaca aaatcgcttc gaggattttc
    30721 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
    30781 tcggtgaatc gaaccgtttt cggatcaaga tcaggatacg catcgtacag tgcttcgcca
    30841 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
    30901 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
    30961 agcttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
    31021 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtacaag
    31081 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagagct gcgtcgagaa
    31141 tggtttcacc gctattagct tccagaacag caccatcagg gcagagatcc tgatgaggca
    31201 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
    31261 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
    31321 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
    31381 tcgtcaatga cctgacgttc tgcgtcgctt aacagcgcgg catcagcagc cagcgcgccg
    31441 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat tcgggctttt
    31501 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
    31561 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
    31621 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccacca
    31681 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
    31741 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
    31801 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
    31861 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
    31921 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
    31981 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
    32041 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
    32101 acacccgcgt ctttaagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
    32161 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
    32221 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
    32281 tgaacgcggt tatcgctacg atcaggaatg cccgcctgct cgcgaatgta atccgccagc
    32341 agatggtcga aatcatcacc accgagcgcg gaatcaccgc cggtcgccag cacttcaaac
    32401 acaccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccgccgag gtcataaacg
    32461 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
    32521 ttaagtaagc gcaggacgtg aaggcccgcc agacgcgccg cgtctttggt gccctgacgc
    32581 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
    32641 gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg caccgggttc
    32701 agcagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
    32761 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
    32821 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
    32881 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
    32941 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
    33001 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
    33061 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
    33121 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
    33181 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
    33241 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
    33301 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
    33361 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
    33421 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
    33481 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
    33541 gctcagcgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
    33601 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
    33661 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
    33721 caaccacact catctttgac gttcgggttg gtgaatttaa acccttcgtt caggccttct
    33781 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
    33841 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
    33901 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
    33961 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
    34021 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
    34081 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
    34141 ttcagcaata tcggtatttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
    34201 ttcggtgacc agggagctgg aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc
    34261 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcatcacgt cgccacaggc
    34321 cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
    34381 acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
    34441 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
    34501 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
    34561 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
    34621 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
    34681 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
    34741 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
    34801 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
    34861 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcctcgc ccattccgac
    34921 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
    34981 ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac agcgcaccga tacctttcgg
    35041 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
    35101 aggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
    35161 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
    35221 ggaaacaagg atggtgtcgt cacgcatcgc tgcttcaagt tctttcaggt cgataatgcc
    35281 gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg cgctccagct gacggcaggt
    35341 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
    35401 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
    35461 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
    35521 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
    35581 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
    35641 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
    35701 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agcgcgatac gccccggcct
    35761 gattctaata tcttgttatc tttaacctaa ctgtttcggg tttcagcaag ttgacaaact
    35821 tacgaatctc cgggagctta cataagtaag tgaccggagt gagtatgtgc agtcaacgca
    35881 gatgcaacac gaaacaggac ggttaaatta agcgcgtaac ttaacgtcga tcgcgtcttg
    35941 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca acacttcctg
    36001 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
    36061 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
    36121 acaacgggta gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
    36181 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
    36241 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
    36301 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
    36361 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
    36421 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagccgggtc aagtctgaca
    36481 ttcccgagta aattggtcaa ctatttactt gactaattta gtcgggtatt taaccttcag
    36541 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
    36601 gacctacggt tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
    36661 tactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
    36721 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
    36781 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
    36841 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
    36901 tgctcgccgt tttcctgagt tgccagccag gccatgcgca cttcataggc aataacctga
    36961 accgccatcg ccaggttcag cgagctgtat tccgggttag ccgcaatcgc gacatgataa
    37021 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
    37081 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
    37141 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
    37201 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgctgcc
    37261 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    37321 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    37381 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    37441 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    37501 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    37561 cgcaaggcgg gtaatttaat tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    37621 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    37681 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    37741 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    37801 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    37861 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcagggc
    37921 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    37981 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    38041 ggcaaactgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    38101 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    38161 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    38221 ggtcataact atatgctgac cggtaacatc gttgctggta acccacgtgt tgtgaaagcc
    38281 atgctggcga acatgcgtga cgagttaagc gacgctctga agcgttaatg actcaggcgg
    38341 gtgatgtcac tcacccgccc tcgcctttca ggcgctattc tgaaatactt cctcaccgct
    38401 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    38461 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    38521 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    38581 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    38641 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    38701 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    38761 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    38821 ggtaaatcaa aaggcacgcc gtcccaggcc ggattgctgg acgatacgca aagtgccatc
    38881 aatgtggtgc gccatcgcag tgatgtaaac ccacaacgtc tggtgctgtt cgggcagagt
    38941 attggcgggg cgaatattct ggatgttatt ggtcggggtg atcgtgaagg catacgtgcg
    39001 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    39061 agtggctact tacttgatga gagttacagc ggcgaaaatt atatcgccag cgtcagcccg
    39121 atcccgcttt tactcattca cggtaaagct gatcacgtta tcccatggca gcacagcgaa
    39181 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    39241 attgatgctt tttccgatcg tcacggcgat gtttatcgcg aacagatggt ggactttatc
    39301 cttagcgcgt tgaatccaca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    39361 accaccttaa gacatgctaa tccactggtc agaacagttt aagatgagaa aaattctgtg
    39421 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    39481 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    39541 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    39601 gttgaccgcc accacggcaa ctttcagcga gcttaatggt gtggatgatg atattgccag
    39661 tcttgatatc agcgaaacag gacaggagat cctgcgctat catcaactca cactgacggc
    39721 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    39781 tcactggcta cgacgtggtt tccgtctgtg tccatcattt attaccagcc atttcacccc
    39841 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    39901 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aaacgcgcga
    39961 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg caacaccacg ctggaattac
    40021 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    40081 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag cctgtaccac ctgatgagcc
    40141 actgtttatg gcactacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    40201 tcagcgagac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    40261 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata cccacctcgc
    40321 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    40381 cgccagactg tacccacgcc tggtgcgcac cacccgcgcg gcgctggccg gatttgaaag
    40441 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    40501 ctggctaatg caggaaaacg atctgcatga aaaacagatt attcttctta ccgggaatga
    40561 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    40621 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    40681 gattattgcg ccttatacca tgccattacc gctcttttca ccaccgctga tctatacgga
    40741 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    40801 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    40861 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gctatactgc
    40921 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta ccctggcgag
    40981 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    41041 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    41101 cgcaaatcgc cgagatcaac agcatatcgc gtgcactaca acggcggaaa agtttattac
    41161 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    41221 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    41281 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggagaacc agcgccaacc
    41341 acgctgacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    41401 ggatgagaaa gccgagcagc atggatgcca cgcccaatgt taacagcgcg aggatcacct
    41461 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    41521 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    41581 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    41641 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt gtcatcattg
    41701 ccagcacgcg caaggcggaa atcaggcgag aaggatcgct gacgcggggc gcgatgagca
    41761 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    41821 ttaaaccaat ccctttaagc cagacgctcc agaaaggtag aaaaatgccg taactaaaaa
    41881 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    41941 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    42001 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    42061 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gtttgccaaa cgttcctgca
    42121 caatggcgat gaaatcgcgt aatgccggct tcatctcccc tttcttccac gccatcagta
    42181 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgtccg gtattaaaat
    42241 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    42301 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    42361 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    42421 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    42481 caaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    42541 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    42601 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    42661 ttggcagtaa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    42721 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    42781 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    42841 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    42901 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    42961 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    43021 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    43081 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    43141 ttaccaactg attgacaccc aaaatggtcg ggtcactccg cgtatttata ccgacccgga
    43201 tatttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    43261 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcggttgt
    43321 cgtagtgcgt cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    43381 catgcgtgtg agttatgcag attgcggcaa cactcgcgcc tttacctgcc cgtatcacgg
    43441 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    43501 acaagggttg tgtaaatccc actggggact aaacgaagtt ccttgtgtgg agagttataa
    43561 agggctgatt tttggcaact gggataccag cgcaccgggc ctgcgtgatt acctgggtga
    43621 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    43681 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    43741 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    43801 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    43861 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct tcactgaaaa
    43921 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    43981 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    44041 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    44101 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    44161 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    44221 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    44281 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    44341 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    44401 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    44461 ggaggtgatg aaatgagtgc gcaagtttca ctagagttac atcaccgcat tagccagttt
    44521 ctctttcacg aagccagctt actggacgac tggaaatttc gtgactggct ggcgcagctc
    44581 gacgaagaga ttcgttacac catgcgcacc acagttaacg cgcaaacacg cgaccgccgc
    44641 aaaggcgtcc agccaccgac aacctggatt tttaatgaca ccaaagacca gctggagcgg
    44701 cgaatcgccc gtctggaaac gggcatggcc tgggcagaag agccgccgtc acgcacccgt
    44761 cacttaatca gcaactgcca gataagcgaa accgacatcc caaacgtatt tgctgtgcgg
    44821 gtaaattatc tgctttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    44881 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    44941 gtcctggatc aagcggtaat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    45001 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggatc gatacctcgc
    45061 ccgtcatcgc cctgttcaac gttggcggcg agttttatgc cattaacgat cgttgcagcc
    45121 atggtaatgc gtcaatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    45181 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    45241 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    45301 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    45361 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caggttgcca cgctggaact
    45421 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    45481 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    45541 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    45601 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaatgttct
    45661 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    45721 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccgc
    45781 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    45841 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    45901 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    45961 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    46021 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    46081 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    46141 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    46201 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    46261 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    46321 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    46381 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    46441 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    46501 ctgctttacc ctacgccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    46561 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    46621 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    46681 accaccgccc gtgcaacgct atcttttaca gcgccaccag caggctggtg tgcgcattct
    46741 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    46801 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    46861 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    46921 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    46981 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    47041 cgcaatgtta gggctaccgc taccgctacc gctaccgcta ctgccgccgc cgtggttctg
    47101 gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    47161 ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct
    47221 tatcggtgcg gtcacgctga atcaggggcg tgagattcgc ccaattcgca aatggatcca
    47281 gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact
    47341 gtaaccagga taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg
    47401 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt
    47461 tgattatgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat
    47521 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    47581 ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    47641 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt
    47701 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt
    47761 tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcat tcctgctgct
    47821 ggcaattacc gggccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg
    47881 gcgtgaacgc cttatccggc atttaataaa ttacagccac tctacccgca acgacatctc
    47941 tgaagttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg
    48001 atcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc
    48061 aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat
    48121 gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    48181 attgttcacc cactggcgcg gcaacggcgc gggctggtta aaatccagtt cctgcggtag
    48241 ctgctcgcaa aactcacccg ccagccactg ctcccgctcc agccagtaac cgctcgcctg
    48301 tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg taccaaatgg
    48361 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    48421 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    48481 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    48541 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    48601 acgattgccg ctcacctggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc
    48661 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat
    48721 tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt
    48781 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    48841 atcagcgcat aggcatcacc tgcctgttcc agcgagaagc gatgggtaat ggcgttacgc
    48901 ggccacagct tccagtccgt cagatcatgg gcgcattttt ccatatggaa cagactggtc
    48961 acccaggagc cgataatccg ccgttgatgg tgcatcagat cggcgctgac ctcgaattcc
    49021 acttttccgg tttcaccaat gtaaaccacc cgtccccagt cagcggtgga ttgcagtgcc
    49081 agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggtg
    49141 agttcggcga taatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcacc
    49201 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt
    49261 gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc
    49321 agcactttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    49381 ccgcaactga taaaagcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    49441 ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    49501 tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag
    49561 ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc
    49621 atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta
    49681 tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa
    49741 atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact
    49801 tcccgcagat cgacggttga atttcctggt aaataagctg ccagcatcgt tttcataaat
    49861 ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg
    49921 tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca
    49981 cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac
    50041 cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac
    50101 cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    50161 ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccag caaaatgccg gtcaccgccg
    50221 ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca
    50281 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc
    50341 ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac
    50401 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg
    50461 cgttcgtcat aaacagcccc attccgcgca gggcgctcca cagccccagt gtggcaacga
    50521 aacttggtac gttaaacacg ccgcgcagca ccccggccag cgttcccatc agcgcgccta
    50581 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    50641 ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    50701 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    50761 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    50821 caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga
    50881 cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat cttcaggttc ctcggtaaaa
    50941 ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa
    51001 actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaact
    51061 cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa
    51121 tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca
    51181 aaatctggct ggaggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag
    51241 aaagcgtgcc gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct
    51301 cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc
    51361 gattggtcag cactgtattt tcgtcaacgc ccagccaggg aatgatcccc gcttctttgc
    51421 ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga
    51481 ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct
    51541 tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac
    51601 gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta
    51661 cggcctgatc cacaatttcc tgaggggcta ccggcgcaat atcaacgtga tcacgcccta
    51721 gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct
    51781 gaccatcgcg cataacggtg gcacaggagg caatgcggcg aatttcttcc atccggtggc
    51841 tgacataaat caccgccacg cccagtgccg acatcttttt caccgcgctg atcaccagtt
    51901 caacttccgc actcgcaagc gaactggtag gttcatcaag aatgaccacg cgcggctcgc
    51961 ccttcatcac ccgcgcaatt tccaccagct gcttttgcgc cgggcttagc gttgaaacaa
    52021 gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca
    52081 tttgcaggta atcaatcatg ccgttgcggc ggggccactg accgaggcag aggttttccg
    52141 ccaccgtcag cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag
    52201 cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat
    52261 caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc
    52321 cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca
    52381 gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct
    52441 ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc
    52501 gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc
    52561 actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg
    52621 cccattttct taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc
    52681 tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga
    52741 ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg
    52801 agatccggcg tggagataat tagtttttca ccaacagaaa tcgctttatc taaaacagtc
    52861 ccttcctgat tagcgacaat ttgcgcgccg ggaacacggg attttaatac ttcttcaaat
    52921 cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacagc aattttcggc
    52981 tggtcaattt tgttggcaat aaaataatcg gcggcagcat tacccagttt tttaccaaat
    53041 tccagcggat cgccgaccag atacgccgag acatatttat cgacgccctt ttgattaata
    53101 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacgacta
    53161 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    53221 tcaacaaagg tactttcttt cgaaatatca ccctgggcgt tagtttcaat taactgtacc
    53281 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    53341 tgggtatcaa ggtatattgc gccaatggtc atttctttat ccgcagccct ggcaaatagt
    53401 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    53461 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    53521 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    53581 aaaggcatca cggtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    53641 tcagcgcgag cagattcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    53701 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga caacaggtca
    53761 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    53821 tgtttcattt cactaaacag ttgctgtgcg atttgttgtt cgcccagcag tcgcagtgcc
    53881 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    53941 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcgtcg cttcagtttc atcgccctgc
    54001 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    54061 ccttcgctta aattctccgg ataatgcagc gcggcatgaa gcagttcgct ggcctgctgc
    54121 ggctgtctgg catcaaggtg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    54181 gtgaccttcc cttccccgcc ttcccacggg tggaatttgc gcgtggcgag aatgtccgcc
    54241 gctttgtctg cctgacccgt gagatgccac aaattgagca actctgctgt catgtcgtcg
    54301 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    54361 ccgcttaact tatcaagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    54421 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgttgctt attccacgca
    54481 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    54541 cataaggtaa tggctttgtt gtagctacgc ttgttgtagt agaagcaggc cagtaaatga
    54601 cgggcaaacc agcactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    54661 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc gcgttcggct
    54721 ttcggcaata aactcgcctg caggtaaagc ggcagcgtgc gctggtagtc cagtgcgttc
    54781 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    54841 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    54901 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    54961 gcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcagg
    55021 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagacca
    55081 gcgtcgaagt taccattacg cgccgccagt cgtgccaggc cataatagcc accggctttg
    55141 ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    55201 cgttcgtaag cactggcgcg aatcaaactg gcctgtccgc actgcggatt tttgttcagc
    55261 gcatgtgcgc gtttcagcgc ctgagtggca tacgccaccg cttgcgggaa atcggcgcgg
    55321 ttatattcca gcatcgccag cgccaggtta cagcggtaat ccagcggatc cagcaccacg
    55381 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    55441 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgcgagtgg cgctttggcg
    55501 acgtccggca acggcaactc ttgcggctga tgttcctgat aactcaggac aatattgccg
    55561 tcggcatctg agagctcaat ggtcagcctt tcaggattga taccgtgcaa cacgccctgg
    55621 atggcggtcg caggcatcag tgccacggca tcatcaagta acgcgttgca tttgccgatt
    55681 tcgcggatcg ccaggcggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    55741 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    55801 aaagaatgat aaggcaggaa atactgctca aaacgcttct cttcgtaagc atcaagccag
    55861 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    55921 ttgtcggtca gactcttgtc ccacgcctgg ccgaattcac tgtgtcccca gctccactgt
    55981 tttttacctg gcgcgatatg gtggttggca acgtgtagca aaccgccatc ttcatcgtga
    56041 caccacgcgc caacaaaatc gtactgtgat ttttcagcca tatatgaggt tggaacgggc
    56101 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    56161 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc cgttacatcc
    56221 ggcgggaaga cgctctgatg cccttccccc cctttcactg ccgggttggc ccaccacaag
    56281 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccaa cgccgcccgt
    56341 tcaggacgca gtgtgaaacc tgtcatcacc tgtaaaccgt gcatcggctc cgtttcgccg
    56401 acccacaccg tctgtgcgcc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    56461 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccagagat ccacggtccc
    56521 agcagcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgttgtttc
    56581 actttatccc atgcgcgatg cacacgaccg cccagttccg gcaggatcat cactttgatg
    56641 tagtcgtttt ccagccacac cgcctgccag gatttcaggg ttttctgctc gctcagcgta
    56701 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    56761 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    56821 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    56881 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    56941 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    57001 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    57061 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    57121 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    57181 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    57241 gtcaggttgc taatgcttgt ttaagtccga aaggtgaatt tgagtattta cagattgatg
    57301 caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    57361 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt gatccggtga
    57421 ccggcgtgtc gcaaaccatg ccgcaagcgc cgtggacaac gccggttgag gtgaagtatc
    57481 tgctggaaga gaagctgggc attcgggtga tggtcgataa tgactgcgtg atgctggcgc
    57541 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    57601 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    57661 gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt
    57721 acggctgcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    57781 taaaatcaca accggttaat actcagcttg atcctgaaaa actgacaacc gcgcagttaa
    57841 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    57901 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    57961 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    58021 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    58081 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    58141 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt ttttcaacag
    58201 attaaccgtt tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag aactttacct
    58261 ttgatgcgct cgataacggc ttcatcattg atgctgtcca gcacgtcaca catccagcca
    58321 gccagttctt tcgcttctgc ttctttgaag ccgcgacgcg taattgccgg agtacccacg
    58381 cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt tttgttgacg
    58441 gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt tttatcaacc
    58501 agatcaacca ggaacaggtg gttatcagtg ccgccggaaa ccactttgta gccgcgctcg
    58561 aggaacactt ctaccatcgc tttagcgttt ttcgcgacct gctgctggta agttttgaac
    58621 tcaggctcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg catcaacgga
    58681 ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc ttcgctacca
    58741 cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt ggtggtagta
    58801 acaacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag gcccgcaacg
    58861 tgcgccatat caacgaacag gtaagcaccg atgctgtcag cgatttcacg cattttcgcc
    58921 cagtccacca cgccggaata tgcagagaaa ccaccgataa tcattttcgg cttgtgttct
    58981 ttggcttgtt tttccagatc ggcgtagtcg atatgaccgg tagcatcgat accgtaagga
    59041 acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag gtgaccgcca
    59101 tgcgccaggt tcatacccag aacggtatca cctggttcca gcagcgcggt gtagaccgca
    59161 aagttagcct gggagccgga gtgcggctgg acgttagcgt agtcagcgcc gaacagttct
    59221 ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc gccgtagtag
    59281 cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg cgcctgcatt
    59341 acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc ctgacgtact
    59401 ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt catttcacgc
    59461 tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt ataggcggtc
    59521 ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt cttgacaaag
    59581 gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa agagcttgca
    59641 accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat cacattcttt
    59701 ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga tgcatttgag
    59761 atacatcaat taagatgcaa aaaaaggaag accatatgct tgacgctcaa accatcgcta
    59821 cagtaaaagc caccatccct ttactggtgg aaacggggcc aaagttaacc gcccatttct
    59881 acgaccgtat gtttactcat aacccagaac tcaaagaaat ttttaacatg agtaaccagc
    59941 gtaatggcga tcaacgtgaa gccctgttta acgctattgc cgcctacgcc agtaatattg
    60001 aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac accagtttcc
    60061 agatcaaacc ggaacagtac aacatcgtcg gcgaacacct gttggcgacg ctggacgaaa
    60121 tgttcagccc gggccaggaa gtgctggacg cgtggggtaa agcctatggt gtactggcta
    60181 atgtatttat caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa gccggtggtt
    60241 gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg cttatcacca
    60301 gcttcgaact ggagccggtc gacggtggcg cagtggcaga ataccgtccg gggcaatatc
    60361 tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga aattcgtcag tactctttga
    60421 ctcgcaaacc ggatggcaaa ggctatcgta ttgcggtgaa acgcgaagag ggtgggcagg
    60481 tatccaactg gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg gttgctccgg
    60541 caggtgattt ctttatggct gtcgcagatg acacaccagt gacgttaatc tctgccggtg
    60601 ttggtcaaac gccaatgctg gcaatgctcg acacgctggc aaaagcaggc cacacagcac
    60661 aagtgaactg gttccatgcg gcagaaaatg gcgatgttca cgcctttgcc gatgaagtta
    60721 aggaactggg gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt cagccgagcg
    60781 aagccgatcg cgctaaaggt cagtttgata gcgaaggtct gatggatttg agcaaactgg
    60841 aaggtgcgtt cagcgatccg acaatgcagt tctatctctg cggcccggtt ggcttcatgc
    60901 agtttaccgc gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt cattacgaat
    60961 gctttggccc gcataaggtg ctgtaatttg atgttgccgg atggaaacat ccggcaaccc
    61021 ttgacgcggt ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga atgacccgtg
    61081 ccacgtcaaa gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc gtgcgaataa
    61141 tggtatcgac acaggtatcg acaatgtcgt ccggtacgac aatctcaatt ttcactttcg
    61201 gcagaaaatc caccatatac tccgcgccgc ggtacagctc ggtatggcct ttctggcgac
    61261 caaagccttt cacttcggtc accgtcatgc cggtaatacc gacttcggcc agtgcttcgc
    61321 ggacatcgtc cagcttgaag ggttttataa tcgcatcaat ctttttcatg ctattccttg
    61381 aaaaggtcgc ctgtcttttg atctgctaaa cgtaacacat aacgccaatt cattccttga
    61441 aatcgtttgc atccagctcg tgtcgggaaa gcagtttata aaattctgtc cggttgcgcc
    61501 ccgccattct cgccgcgtgg gtgacgttgc ctttggtgat ttgcagcagc ttacgcaaat
    61561 agttgagttc aaactgatta cgtgcctcaa caaaggttgg cagcgccgtg ttttcaccct
    61621 ccagcgcctg ctctactaat gcatcgctaa taaccggaga tgaggtcagc gccacgcact
    61681 gttcaatcac gttgaccaac tggcgcacat tacccggcca gctagcggtc atcaggcgtt
    61741 tcatcgcatc agtggagaac gcacggacaa acggtttatg tcgctctgcc gcctggcgca
    61801 acaggtgatt tgccagaagc ggaatgtctt ctgtgcgctc tgccagagcc ggaattttca
    61861 ggctgacaac gttgaggcgg taatagagat cttcgcggaa ttccccgcgc gccatcgctt
    61921 ttggcagatc acggtgagtg gcagaaataa tccgcacatt gatatcaata tcgcggttac
    61981 tgcccagcgg gcgcacttta cgctcctgca acacgcgcag cagtttgacc tgtaacggcg
    62041 caggcatatc gccaatttca tcgagaaata gcgttccgcc ttccgccgcc tggaataaac
    62101 cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac agctccgact
    62161 ccagcaattg ctcgggcaat gcgccacagt taatagcaat aaatggtttg ctgttgcgcg
    62221 ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc tgaccgttaa
    62281 tcaaaacgct gacgtctgat tgcgccacca gccgcgcctg ttccagcaaa cgcagcatca
    62341 gcgggctgcg ggtgacaatt gcctcgcgcc agcgttcatc ggtggctggc gcggattgct
    62401 ccagcgcatc gtcaattgcc tgatatagcg cgtctttgtc gacaggcttg gtgaggaaac
    62461 taaaaacgcc ctgctgtgtt gcagcaacgg catcggggat agaaccatgc gcagtaagaa
    62521 taattactgg cattcccggc tgcactttct ggatttcagc aaatagctgc ataccgtcca
    62581 tttcatccat ccgcaggtcg ctgataacta aatctacttt ttcgcgattc agtacccgta
    62641 atccttcagc gccactttcc gccgtgacca cgctgtagcc ttcgctggtc aggcgcaggc
    62701 caagcagttt cagcaatccc ggatcgtcat cgaccaataa taaatgcgca ggtttatggc
    62761 tcatcaggag tgacctcatg ggtggatggc gcgggctttt caccctcgtg cggcgtatcc
    62821 ggcgagaaat ttccagccgg tttgcgggtc gagagctggc gttcaatatc agtcaggttt
    62881 tccagcttgc gggtggtgag ttccagttgc tgctgaagaa catgatgttg ctggcgcaat
    62941 gtatccagct cgctgtcgct ggactgctgg agtttactgt aacgttggcg ctcttccgcc
    63001 agttgcagtt gcagcgcctg accgtcgcgc cagagttgat acagtgggcg aacctgtgcc
    63061 gggatctcgg tacttaacgc ttcaatacgc gcgaccagct ggcggcgctc atatggcgta
    63121 attttggcgt cggcgagcaa aatcccttgt ttaaaggtat tttgccagct gccgtcgtca
    63181 tattggcgag cttgctgacg cgactgcgca ggcattaaac gatcagcaca atccatcgcc
    63241 cgcagccagt aaagcggatt ggtttcggtt gatttacctt gcagcgccca gatgtcgcta
    63301 cattcagtag aaagatagtc agccagttga taaaccggaa ttttttcttc tgctggcgta
    63361 tcaatggctg gcttattgtg attctgcacg caacccagca atgccagaca tggcagccct
    63421 gccagccaca gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt caccagactt
    63481 aaagcctatc ccagtgggcg taattgttgc aggcagtttg gacatggaca gcgcggagaa
    63541 accggagcgt acacgtcgta cgtgaggatt tcgagcactg cccggggccg aactggcaaa
    63601 taaaatagcc tgatgggata ggctgttagc attatttcgt gtttttcgac gacggcaatt
    63661 caatgcggaa acaaacgtct tgcccgctct cgtcgaccag atacagttcc ccttgcatac
    63721 ggcgaataca atccctggca atgcttaatc ccagaccgct gcccttcacc gccccttttc
    63781 gctggtggct tccctgaaaa aagggttcga agatcatggc gcgttcctct tgcggaatgg
    63841 gcgtgcctgt attgatgaca tcaatataaa cccgcgcacc atgtgaactg ctgcgaaggc
    63901 aaatgttacc ggattcagcc ccgtagtgca ccgcattgga gtaaagatta tccagtacgc
    63961 tcatcagcag cattggctcc gccaggcaag ctgttgcttt gagatcgacg tcggtatgca
    64021 tcattttagc ccgtgcgggc aggctatgag cagaaactac tgtctccacc agcggtgcta
    64081 actcaacatt ctccagttcc accgcactgt ccgcctgttt acggttgtaa tcaagcagtt
    64141 gttcgatcag tttttgcaaa ttgcggctgc tgctatcaag aatgctcacc acctcttttt
    64201 gctctggcgt aagcggccca acaacctggt cagccagtaa ttcagtgccc tcgcgcatac
    64261 tcgccagtgg cgtttttaat tcatgagata aatgtcttaa aaattgatgg cgttgggatt
    64321 ccagccatga caggcgctca cttaaccaaa gaatacgttg cccaaccgag cgtaactcgc
    64381 tcggtccact gaacgagacg ctattgccca gagaacgccc ttcccccaga cggttgatca
    64441 tgcgctcgat atttttcacc ggaccaataa tcatccgcgt gaaaagcagt accattacca
    64501 gactcaccag aaatagcacc agcgattgcc aaccaaaata ttgcccacgt tcggcgattt
    64561 cacgctgaag ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc accatttcgg
    64621 tattggcact ggcaaaggct tcaagacgcg cggcggcggc agcatcggga ccactgttgt
    64681 tacactgaag ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa agtttatcgt
    64741 ctggcagtac gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc tggctttgat
    64801 aaaccttcgc aagcgtagga tcgtccagca cgcaatactg acggtagcta cgctccatct
    64861 ccagcgctgc gttggtcatt gcttcgctgc gccgggcatc gataagcgtg gtgcggttga
    64921 ccagcgccgc ctggtcgcta agcgcattca ggctttgcca ggcttgccat gccagcacca
    64981 acaggggcag cagaatcagc aaaaatgcca gcattaccag ttgtcgtaat gagcggggaa
    65041 aaacgggcca gcgtttcaag gtgttactct cgtcagacgc gaatagcctg atgctaaccg
    65101 aggggaagtt cagatacaac aaagccggga attacccggc tttgttatgg aataaggcgg
    65161 tgcctaactc gacgtttcgc ccgatggttg aaatagctac gctgatatca gaagttggac
    65221 ggcaggcacc ttgttgtgcg tcattcgtat tttatgtagc acgtcccgaa ggggctgaca
    65281 taagtcggtg aatgagccac tgcttactat tatgcagtaa ctgtgccaat aaagaaaata
    65341 gtttggtaac gtactgatta tacgctgctt tgaggggttt atggcttctc cgctgtttga
    65401 atgtaaatca gccactgtgt cgctaaaaag agacaactta agataaactt attagattac
    65461 attaaaatca atcatttaag tgtcgccaga aagcgacgcg gtaaaccacc cattgtcgtg
    65521 atttacagac acaaaaaagc tccctgagtt gggagcttat aatagcggtt ggtatttaat
    65581 gccggatgcg gcgtgaatgc cgcatccggg ctgcaaaacc aatgggctga cgacttaccc
    65641 caactgctta cgcgcattgc ggaaaatacg catccatggg ccatcctcgc cccagttttc
    65701 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    65761 gactcgacca ctttcagtcg tgactgccgt aataccgttc ggtgaaccgt tcgggttagc
    65821 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    65881 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    65941 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagcgacg ggctttgggt
    66001 cacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtattgcgca caaaacgtgg
    66061 ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    66121 tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat cgcgtacacg
    66181 gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    66241 gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc cagccagcag
    66301 gtcgctcata tgcacatcga ttgcatcgaa gcctgcacgg tggaaggctg ccgccatttc
    66361 aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcgggcgcg cgccagtggc
    66421 aataaacggt gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg
    66481 atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt tgtcacgcag
    66541 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct
    66601 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac
    66661 ataatggacg caatcagcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    66721 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacaccg ccaggcgatc
    66781 gtcacccaga gagccgatat ccgcatcaat gccacaatga ccagcaaagg ccatttccgc
    66841 cagtgtgacc aacagaccgc catcagagcg gtcgtgatac gccagcagct tacgctgtgc
    66901 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacatcgc gtacatctgc
    66961 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    67021 gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtagaaagct gtggcgtgat
    67081 ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    67141 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    67201 caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt taacggcttc
    67261 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    67321 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    67381 cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat
    67441 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    67501 ctgccacgga cccaccatct gatcgcgcgc taccatgcca gttacgctgc ggtcaccgat
    67561 ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    67621 tgcaatggtt atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    67681 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    67741 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    67801 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    67861 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcctgg
    67921 ctcgtcgctc agaatgtcgc gcagttcaaa tttaccgcca cgcccgccgt cgctcaccag
    67981 ttccggcata gcgttagaaa gaccgcctgc gccaacgtcg tggataaaca gtattgggtt
    68041 ggcatcacca agctgccagc aacggtcaat cacttcctgg cagcgacgct ccatctccgg
    68101 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga
    68161 agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    68221 cgcgccaacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    68281 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc tgttcacttt
    68341 ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc
    68401 cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc gctcaggctt
    68461 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg aaacggagaa
    68521 accaaccaaa cccgctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc
    68581 accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    68641 ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcatagcggc ccgtttcgtg
    68701 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    68761 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt
    68821 cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc
    68881 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    68941 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt
    69001 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg
    69061 ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat
    69121 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    69181 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    69241 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    69301 gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca ggcgttcaag
    69361 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    69421 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    69481 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    69541 gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    69601 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    69661 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    69721 ctttcactat tattacgttt tttcaagct
//
LOCUS       DAZFAH010000002        68392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFAH010000002 DAZFAH010000000
VERSION     DAZFAH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.002"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHJWB_000062"
     CDS             complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHJWB_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO4311769.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
     gene            188..682
                     /gene="napF"
                     /locus_tag="ACHJWB_000063"
     CDS             188..682
                     /gene="napF"
                     /locus_tag="ACHJWB_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4311770.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            672..935
                     /gene="napD"
                     /locus_tag="ACHJWB_000064"
     CDS             672..935
                     /gene="napD"
                     /locus_tag="ACHJWB_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4311771.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            932..3418
                     /gene="napA"
                     /locus_tag="ACHJWB_000065"
     CDS             932..3418
                     /gene="napA"
                     /locus_tag="ACHJWB_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO4311772.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3425..4120
                     /gene="napG"
                     /locus_tag="ACHJWB_000066"
     CDS             3425..4120
                     /gene="napG"
                     /locus_tag="ACHJWB_000066"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4311773.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4107..4970
                     /gene="napH"
                     /locus_tag="ACHJWB_000067"
     CDS             4107..4970
                     /gene="napH"
                     /locus_tag="ACHJWB_000067"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4311774.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4967..5416
                     /gene="napB"
                     /locus_tag="ACHJWB_000068"
     CDS             4967..5416
                     /gene="napB"
                     /locus_tag="ACHJWB_000068"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4311775.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5426..6028
                     /gene="napC"
                     /locus_tag="ACHJWB_000069"
     CDS             5426..6028
                     /gene="napC"
                     /locus_tag="ACHJWB_000069"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4311776.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHJWB_000070"
     CDS             6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHJWB_000070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4311777.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHJWB_000071"
     CDS             6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHJWB_000071"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4311778.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHJWB_000072"
     CDS             7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHJWB_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4311779.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHJWB_000073"
     CDS             8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHJWB_000073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4311780.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHJWB_000074"
     CDS             8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHJWB_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4311781.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHJWB_000075"
     CDS             8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHJWB_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4311782.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAAAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFNFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHJWB_000076"
     CDS             10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHJWB_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4311783.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHJWB_000077"
     CDS             11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHJWB_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4311784.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIARAMQHLSPQESK"
     gene            complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHJWB_000078"
     CDS             complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHJWB_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4311785.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13329..15920
                     /gene="yejO"
                     /locus_tag="ACHJWB_000079"
                     /pseudo
     CDS             13329..15920
                     /gene="yejO"
                     /locus_tag="ACHJWB_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16024..16100)
                     /locus_tag="ACHJWB_000080"
     tRNA            complement(16024..16100)
                     /locus_tag="ACHJWB_000080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16064..16066),aa:Pro,seq:ggg)
     gene            complement(16175..17935)
                     /gene="yejM"
                     /locus_tag="ACHJWB_000081"
     CDS             complement(16175..17935)
                     /gene="yejM"
                     /locus_tag="ACHJWB_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4311786.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17955..18182)
                     /gene="yejL"
                     /locus_tag="ACHJWB_000082"
     CDS             complement(17955..18182)
                     /gene="yejL"
                     /locus_tag="ACHJWB_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4311787.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18364..19371
                     /gene="yejK"
                     /locus_tag="ACHJWB_000083"
     CDS             18364..19371
                     /gene="yejK"
                     /locus_tag="ACHJWB_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4311788.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19510..19794)
                     /gene="rplY"
                     /locus_tag="ACHJWB_000084"
     CDS             complement(19510..19794)
                     /gene="rplY"
                     /locus_tag="ACHJWB_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4311789.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19919..21679)
                     /gene="radD"
                     /locus_tag="ACHJWB_000085"
     CDS             complement(19919..21679)
                     /gene="radD"
                     /locus_tag="ACHJWB_000085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4311790.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVACMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21828..22523
                     /gene="rsuA"
                     /locus_tag="ACHJWB_000086"
     CDS             21828..22523
                     /gene="rsuA"
                     /locus_tag="ACHJWB_000086"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4311791.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22551..23741
                     /gene="bcr"
                     /locus_tag="ACHJWB_000087"
     CDS             22551..23741
                     /gene="bcr"
                     /locus_tag="ACHJWB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4311792.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            24074..24418
                     /gene="yejG"
                     /locus_tag="ACHJWB_000088"
     CDS             24074..24418
                     /gene="yejG"
                     /locus_tag="ACHJWB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4311793.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24422..26011)
                     /gene="yejF"
                     /locus_tag="ACHJWB_000089"
     CDS             complement(24422..26011)
                     /gene="yejF"
                     /locus_tag="ACHJWB_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4311794.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     ASGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26013..27038)
                     /gene="yejE"
                     /locus_tag="ACHJWB_000090"
     CDS             complement(26013..27038)
                     /gene="yejE"
                     /locus_tag="ACHJWB_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4311795.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27038..28132)
                     /gene="yejB"
                     /locus_tag="ACHJWB_000091"
     CDS             complement(27038..28132)
                     /gene="yejB"
                     /locus_tag="ACHJWB_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4311796.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28133..29947)
                     /gene="yejA"
                     /locus_tag="ACHJWB_000092"
     CDS             complement(28133..29947)
                     /gene="yejA"
                     /locus_tag="ACHJWB_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4311797.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            complement(30029..31585)
                     /gene="pdeN"
                     /locus_tag="ACHJWB_000093"
     CDS             complement(30029..31585)
                     /gene="pdeN"
                     /locus_tag="ACHJWB_000093"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4311798.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINTIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31766..32332)
                     /gene="mepS"
                     /locus_tag="ACHJWB_000094"
     CDS             complement(31766..32332)
                     /gene="mepS"
                     /locus_tag="ACHJWB_000094"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4311799.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(32744..33457)
                     /gene="lpxT"
                     /locus_tag="ACHJWB_000095"
     CDS             complement(32744..33457)
                     /gene="lpxT"
                     /locus_tag="ACHJWB_000095"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4311800.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNHLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(33496..34482)
                     /gene="yeiR"
                     /locus_tag="ACHJWB_000096"
     CDS             complement(33496..34482)
                     /gene="yeiR"
                     /locus_tag="ACHJWB_000096"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4311801.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(34600..36066)
                     /gene="yeiQ"
                     /locus_tag="ACHJWB_000097"
     CDS             complement(34600..36066)
                     /gene="yeiQ"
                     /locus_tag="ACHJWB_000097"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4311802.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(36289..36861)
                     /gene="yeiP"
                     /locus_tag="ACHJWB_000098"
     CDS             complement(36289..36861)
                     /gene="yeiP"
                     /locus_tag="ACHJWB_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4311803.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            37016..37270
                     /gene="yeiW"
                     /locus_tag="ACHJWB_000099"
     CDS             37016..37270
                     /gene="yeiW"
                     /locus_tag="ACHJWB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4311804.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(37267..38448)
                     /gene="setB"
                     /locus_tag="ACHJWB_000100"
     CDS             complement(37267..38448)
                     /gene="setB"
                     /locus_tag="ACHJWB_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4311805.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            38816..39946
                     /gene="fruB"
                     /locus_tag="ACHJWB_000101"
     CDS             38816..39946
                     /gene="fruB"
                     /locus_tag="ACHJWB_000101"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4311806.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKDHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            39946..40884
                     /gene="fruK"
                     /locus_tag="ACHJWB_000102"
     CDS             39946..40884
                     /gene="fruK"
                     /locus_tag="ACHJWB_000102"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4311807.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            40901..42592
                     /gene="fruA"
                     /locus_tag="ACHJWB_000103"
     CDS             40901..42592
                     /gene="fruA"
                     /locus_tag="ACHJWB_000103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4311808.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMLYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            43016..43957
                     /gene="psuK"
                     /locus_tag="ACHJWB_000104"
     CDS             43016..43957
                     /gene="psuK"
                     /locus_tag="ACHJWB_000104"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4311809.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            43945..44883
                     /gene="psuG"
                     /locus_tag="ACHJWB_000105"
     CDS             43945..44883
                     /gene="psuG"
                     /locus_tag="ACHJWB_000105"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4311810.1"
                     /translation="MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            44977..46227
                     /gene="psuT"
                     /locus_tag="ACHJWB_000106"
     CDS             44977..46227
                     /gene="psuT"
                     /locus_tag="ACHJWB_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4311811.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46295..46993)
                     /gene="yeiL"
                     /locus_tag="ACHJWB_000107"
     CDS             complement(46295..46993)
                     /gene="yeiL"
                     /locus_tag="ACHJWB_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4311812.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            47123..48064
                     /gene="rihB"
                     /locus_tag="ACHJWB_000108"
     CDS             47123..48064
                     /gene="rihB"
                     /locus_tag="ACHJWB_000108"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4311813.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            48164..49414
                     /gene="nupX"
                     /locus_tag="ACHJWB_000109"
     CDS             48164..49414
                     /gene="nupX"
                     /locus_tag="ACHJWB_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4311814.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49470..50558)
                     /locus_tag="ACHJWB_000110"
     CDS             complement(49470..50558)
                     /locus_tag="ACHJWB_000110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4311815.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISCSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMLVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(50562..51419)
                     /gene="nfo"
                     /locus_tag="ACHJWB_000111"
     CDS             complement(50562..51419)
                     /gene="nfo"
                     /locus_tag="ACHJWB_000111"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4311816.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(51493..52542)
                     /gene="yeiH"
                     /locus_tag="ACHJWB_000112"
     CDS             complement(51493..52542)
                     /gene="yeiH"
                     /locus_tag="ACHJWB_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4311817.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            52641..53522
                     /gene="yieE"
                     /locus_tag="ACHJWB_000113"
     CDS             52641..53522
                     /gene="yieE"
                     /locus_tag="ACHJWB_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4311818.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            53727..55196
                     /gene="lysP"
                     /locus_tag="ACHJWB_000114"
     CDS             53727..55196
                     /gene="lysP"
                     /locus_tag="ACHJWB_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4311819.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            55488..57467
                     /gene="cirA"
                     /locus_tag="ACHJWB_000115"
     CDS             55488..57467
                     /gene="cirA"
                     /locus_tag="ACHJWB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4311820.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYLF"
     gene            complement(57499..58335)
                     /gene="yeiG"
                     /locus_tag="ACHJWB_000116"
     CDS             complement(57499..58335)
                     /gene="yeiG"
                     /locus_tag="ACHJWB_000116"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4311821.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            58593..59261
                     /gene="folE"
                     /locus_tag="ACHJWB_000117"
     CDS             58593..59261
                     /gene="folE"
                     /locus_tag="ACHJWB_000117"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4311822.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            59278..60435
                     /gene="yeiB"
                     /locus_tag="ACHJWB_000118"
     CDS             59278..60435
                     /gene="yeiB"
                     /locus_tag="ACHJWB_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4311823.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            60577..61617
                     /gene="galS"
                     /locus_tag="ACHJWB_000119"
     CDS             60577..61617
                     /gene="galS"
                     /locus_tag="ACHJWB_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4311824.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            61897..62895
                     /gene="mglB"
                     /locus_tag="ACHJWB_000120"
     CDS             61897..62895
                     /gene="mglB"
                     /locus_tag="ACHJWB_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4311825.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            62956..64476
                     /gene="mglA"
                     /locus_tag="ACHJWB_000121"
     CDS             62956..64476
                     /gene="mglA"
                     /locus_tag="ACHJWB_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4311826.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            64492..65502
                     /gene="mglC"
                     /locus_tag="ACHJWB_000122"
     CDS             64492..65502
                     /gene="mglC"
                     /locus_tag="ACHJWB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4311827.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(65745..66980)
                     /gene="preA"
                     /locus_tag="ACHJWB_000123"
     CDS             complement(65745..66980)
                     /gene="preA"
                     /locus_tag="ACHJWB_000123"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4311828.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPSEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(66974..68212)
                     /gene="preT"
                     /locus_tag="ACHJWB_000124"
     CDS             complement(66974..68212)
                     /gene="preT"
                     /locus_tag="ACHJWB_000124"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4311829.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT        16434 a        16773 c        18322 g        16863 t
ORIGIN      
        1 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
       61 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
      121 cttattaatt atgcggttta tttggccgct ctcaattttc agagcgcgtt aatgatggaa
      181 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
      241 cagtaacggt atccgtccgc cttggtccgg tgatgaatct cattttctga cccattgcac
      301 ccgttgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
      361 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc
      421 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
      481 tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
      541 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
      601 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
      661 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
      721 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
      781 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
      841 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
      901 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
      961 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
     1021 cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc gtttctgcgg
     1081 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
     1141 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
     1201 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
     1261 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
     1321 attcaaaacc gccctgaaag aaaaaggacc ggaatcgatc ggtatgttcg gttctggtca
     1381 gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct tccgttcgaa
     1441 caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
     1501 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
     1561 gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca tcactaaccg
     1621 tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
     1681 cgagctggcg gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta
     1741 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaagcacgt
     1801 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
     1861 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
     1921 agccttcgtt gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga
     1981 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
     2041 gacgatgggc ttcaaccagc atactcgtgg cgtgtgggcc aacaacctgg tctacaacct
     2101 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
     2161 gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
     2221 catggtggtg actaacgaga aacaccgtga tatttgcgag aagaagtgga atatcccgag
     2281 cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cactgaaaga
     2341 cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
     2401 taatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
     2461 gtatccgaca gtcagtgcgc tggcagccga cttgatcctg ccgaccgcaa tgtgggtaga
     2521 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaggtaca
     2581 ggcgccaggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
     2641 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
     2701 gctgtacgaa gttctgtatg ccacccccga agtgagcaaa taccctgtat ccgaactggc
     2761 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
     2821 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
     2881 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
     2941 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggtaaacc
     3001 ggatggcaaa gcggtgatct tcgcactgcc gttcgaaccg gcggcggaag caccggatga
     3061 agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat
     3121 gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc
     3181 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
     3241 tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt
     3301 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg atgcgaccga
     3361 tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc
     3421 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt
     3481 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
     3541 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
     3601 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
     3661 gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
     3721 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga
     3781 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
     3841 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
     3901 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
     3961 gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtat gcgtgctgga
     4021 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gtcaccatta
     4081 ccgcttcggt tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
     4141 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
     4201 agttcttcgt gctggggatg tttttgagcg gtccatggtt tggtgtgtgg atcttgcacg
     4261 gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
     4321 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
     4381 tcaccgtgct ctacgccctg gcgggaaagc gattattttg cagctgggtc tgcccgctga
     4441 acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
     4501 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctggtggga tcagccttaa
     4561 ctggcacgct catttgggaa tggatcaacc ccgtttctct gatggggcgt agcctggtaa
     4621 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
     4681 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
     4741 gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt
     4801 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
     4861 cggtgcaggt caccagccgc gattgcatga cgtgtggtcg ctgcgtggat gtctgttctg
     4921 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
     4981 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
     5041 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cgcaggaagg
     5101 ggccattcgg atgccaaaag agcaggatcg gatgccgctg aactatgtga atcagccgcc
     5161 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
     5221 gtgccacggt gtcgaaagct atcgcaccac tggcgcgccg cgtatcagtc ctactcactt
     5281 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
     5341 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
     5401 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
     5461 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
     5521 cggttttgtt ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa
     5581 taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat
     5641 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
     5701 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
     5761 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
     5821 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
     5881 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
     5941 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga
     6001 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
     6061 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
     6121 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
     6181 tttgctgact gggttgtctc gccctgacgc tggcgaggtt ctctggcaag ggcagccctt
     6241 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
     6301 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgatgccgc
     6361 acaatgtctg gaagctctgg cgcaggccgg gcttgccgga ttcgaagata ttccggtaaa
     6421 tcaactttct gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
     6481 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
     6541 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
     6601 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgggggccgc
     6661 atgatgttct ggcgcatttt ccgtcttgag ctgcgcgtag cgtttcgcca tagcgccgaa
     6721 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
     6781 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt
     6841 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
     6901 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
     6961 tggatggtaa ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga
     7021 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
     7081 ggctttctcg gtgcaccggg tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg
     7141 ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg
     7201 gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
     7261 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
     7321 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
     7381 atcaactggc gatccctcca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
     7441 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
     7501 ctgattatca gcagggaaat agctaccgca ttatctactt gcatgtgcct gcggcgatct
     7561 ggtcgatggg catttatgca tcaatggcgg tggcggcttt tattggcctt gtctggcaga
     7621 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
     7681 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
     7741 atgcacgtct gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt
     7801 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
     7861 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
     7921 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
     7981 tttttggctt cctgctgctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
     8041 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
     8101 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
     8161 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
     8221 gcagcatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg
     8281 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
     8341 tgtgccgtgt tggcagggct ggcgctgact atcggtctgg tgctatatgc gctgcgctcg
     8401 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa
     8461 atgccggaag tcgggcagcg tctgcgcgtc ggcgggatgg tgatgccggg tagcgtgcag
     8521 cgcgatccca attcgctaaa agtaactttc accatttacg acgctgaagg ttcagtagat
     8581 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
     8641 ggcgaactgg aaaaaggcaa tcatatcctc gcaaaagaag tgctggcgaa acacgacgaa
     8701 aactacacgc cgccagaagt tgagaaagcg atggaagcca atcaccgtcg cccggcgagt
     8761 gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg tgtctggcgc
     8821 taggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtagcgcgc ggagatgcgc
     8881 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctgccgcat
     8941 ttctggtact ggtcaatgcc ttcgtggtca acgacttcac cgtcacctat gttgccagca
     9001 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag
     9061 gctcgctcct gctgtgggtg ctgctgatga gcggctggac ctttgcggta gcgattttta
     9121 gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg atggtcagcg
     9181 tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ttgccgaact
     9241 tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
     9301 cgcctctgct ctatatgggg tacgtggggt tctcggtggc gtttgctttt gccattgctt
     9361 ctttgttgag cgggcgtctg gacagcactt atgcgcgttt tactcgtccg tggacgctgg
     9421 cggcgtggat cttcttgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
     9481 tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
     9541 tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
     9601 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg
     9661 tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt
     9721 ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg
     9781 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag
     9841 cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc
     9901 tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
     9961 tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct
    10021 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc
    10081 tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    10141 tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta
    10201 tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct caccttgggc
    10261 tggcagtgac aattgttggc attgccttta gtcagaacta tagcgttgag cgtgatgtgc
    10321 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaacttc cgtgatgtca
    10381 aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac tatcggcgta acgcgagagg
    10441 gtaaaccgga aacagtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    10501 tgaccgaagc ggcgattgac ggtggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    10561 agctggaaaa cggcgcgtgg gctgtgcgtc tttactacaa accatttgtt cgctggattt
    10621 gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat cctcgctatc
    10681 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggctgt atgaagcgca aagtattgtt
    10741 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagt tggcgcgtaa
    10801 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc ctgtgccgaa
    10861 gtttcgtctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    10921 gggcaaacca gtgctgctta acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca
    10981 tcaatatctg aatcagcttt ctgcgcaggg catccgagtg gtcggcatga actataaaga
    11041 cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg cgctaagcct
    11101 gtttgatggc gacggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacctt
    11161 ccttattgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    11221 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    11281 tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg
    11341 ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga agaactgcgc
    11401 tgcccgaaat gccagaataa cagcattgcc gattccaact cgatgattgc caccgacctg
    11461 cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta agaaagagat tgtcgattat
    11521 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    11581 ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat ttacgcccgc
    11641 tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg aacaaagcgt gccggaaggt
    11701 aagcgtgccg gatatgtcgc ttatttgccg ggcattgtgg tggcgttaat tgtggctggc
    11761 gtcagctact accagaccgg aaattatcaa caggtgaaaa tctggcagca ggccacggca
    11821 caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa
    11881 gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    11941 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgctag tatcgccacc
    12001 gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gcgatgccgc actgggttat
    12061 gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggcgg cgaactgctg
    12121 cgccagttgg tgagaagcga ccacagcaat atccgtgtgt taagcatgta tgcgtttaat
    12181 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    12241 ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgcg ggcgatgcaa
    12301 catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctatt
    12361 tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat
    12421 tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca
    12481 ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca tccagctcgc
    12541 gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt
    12601 actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca
    12661 gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagtg
    12721 caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat
    12781 ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca
    12841 agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt
    12901 cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg cgtcgcataa
    12961 gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata gtagtctcct
    13021 gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga
    13081 atgttacccc attttatgta tggacttgta gtgaaaacta caaccttgtc gttcgctttg
    13141 gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga aaaacaacta
    13201 aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact gactccgggt
    13261 atggagctat gggtattttc cgtacccaat gcttttaaca gcaattaatt tcataggatg
    13321 aaagctcaat tcatcaatct ggttctgttt ctctttgtcg ttccgcaata tctgttctgg
    13381 tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat ggtgagacag
    13441 ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc aataatacga
    13501 aaatcaatcc tggcggagaa cagcatataa aagaatttgg tgtaagtagt aatactgaaa
    13561 ttaaaggagg gtatcagtac attgaaatga atggtaccgc agaatactca gtattaaatg
    13621 acggttatca aattgttcaa atgggtggcg cggcaaacca gactaccctc aataatggtg
    13681 tgctacaggt ttatggcgca gcgaatgatc ccacgattaa aggcgggcgc ttaatcgttg
    13741 aaaaagatgg gattaccgtc cttgccgcta tcgaaaaggg aggattactg gaggttaaag
    13801 aggggggatt agcgattgcg gtagatcaga aagcaggcgg tgctattaaa gcaagcacgc
    13861 gggtcatgga ggcattcgga acaaaccgtc tcggtcagtt cgaaatcaag aatggtattg
    13921 ctaacaatat gctgttggaa aacggcggaa gtttgcgagt tgaagaaaat gacttcgctt
    13981 ataatactac tgtagatagt ggcggcttac tggaggttat ggatggcggg actgtaactg
    14041 gcgttgataa aaaagcaggc ggaaaattaa ttgtctcaac gaatgcgctg gaagtgagtg
    14101 gtacaaacag taaaggccaa tttagtataa aagatggtgt gtcaaaaaat tatgaactgg
    14161 atgatggttc cgggctcatt gttatggagg acacgcaggc cattgatact atccttgatg
    14221 agcatgccac tatgcaatcg ctgggaaagg atactggtac gaaagtgcag gcaaatgcgg
    14281 tatatgatct cggtcgatca gatcagaatg gaagtatcac gtattcctca aaagccatct
    14341 ctgaaaatat ggttatcaac aatggccgcg ctaacgtctg ggctggcaca atggttaacg
    14401 tttcagtcag agggaatgat ggcattcttg aggtcatgaa gccgcaaata aattatgcac
    14461 ccgcaatgtt ggtgggtaag gtagtggttt ctgagggcgc ttcttttaga acgcatggtg
    14521 ccgtggatac cagcaaagcg gacgtttcgc tcgaaaatag cgtatggacc atcattgccg
    14581 atatcactac gacgaaccaa aacaccctcc tcaacttagc caaccttgcg atgtctgacg
    14641 caaatgtgat tatgatggat gagccagtga ctcgttcatc agtgacggca agtgcggaaa
    14701 atttcattac gttgaccacc aataccctgt cgggaaacgg caatttttat atgcgtaccg
    14761 atatggctaa tcatcagagc gatcagctca acgtcaccgg tcaggcaaca ggtgatttca
    14821 aaatattcgt gacggacacc ggtgccagcc cggcagcagg agatagcctt acactggtaa
    14881 caacgggcgg cggtgatgct gcatttacgt tgggcaatgc cggaggcgtt gttgatatcg
    14941 gtacgtatga atataccttg ctggataatg gcaaccatag ctggagtttg gcagagaatc
    15001 gcgcgcaaat taccccttca accactgatg tgctgaatat ggcggcagca caaccgctgg
    15061 tatttgatgc agaactggac accgtgcgtg agcgtcttgg tagcgtaaaa ggcgttaatt
    15121 acgatacggc gatgtggagt tcggcaatta acacccgcaa caacgtgacc actgatgcgg
    15181 gcgctggctt tgagcaaaca ttgaccgggc tgacgctcgg tatcgatagc cgtttctccc
    15241 gtgaagaaac cagcacaatt cgcggcttgt tctttggtta ctctcattct gatattggtt
    15301 ttgatcgcgg cggcaaaggc aatgtcgata gctataccct gggggcttat gccggttggg
    15361 agcatcagaa cggtgcctat gttgatggag tggtgaaagt tgaccgtttt gccaacacca
    15421 tccattgcaa gatgagtaat ggggcaacag cgtttggcga ttacaatagt aacggcgcgg
    15481 gtgctcatgt cgagagcggg ttccgttggg ttgacggatt gtggagtgtt agaccctatc
    15541 tggcctttac cggctttacc acagatggtc aggactacac gttatcaaac ggcatgcgcg
    15601 cggatgtggg aaatacccgg atattacgcg ctgaagcggg aacggcggta agctatcaca
    15661 tggacctgca aaacggtacg acgctggaac cctgactgaa agccgccgtg cgtcaggaat
    15721 acgccgattc taaccaggtg aaagttaatg acgatggcaa atttaataat gatgtggctg
    15781 gaacccgtgg cgtttatcag gctgggataa ggtcatcgtt taccccgacg ttaagcggtc
    15841 atttgtcagt cagctatggc aatggcgcag gggtagaatc gccgtggaat actcaggcgg
    15901 gtgtggtctg gacgttctga taacaaaaaa taaacaggct gtgatgtgtc acggtctgtt
    15961 tatcgaatta attgcagata taaaaaaaac caaccgtaaa ggttggtttt ttcttgggat
    16021 ttttggtcgg cacgagagga tttgaacctc cgacccccga caccccatga cggtgcgcta
    16081 ccaggctgcg ctacgtgccg actcgtggct gctaatacta ccgttttcca caccgattgc
    16141 aagtaagata ttttgctaac tgatttataa ttaatcagtt agcgataaaa cgcttctcgt
    16201 ctgtcagcac ttgcaataac aaacttaact gtggtttttc atctttcact ctttcaccac
    16261 gtaagttgta agtgcggtat ttaccgttat tgttcagcac cagcgtcttt ttcggggtgg
    16321 taattgccag cgtatcgtta tccgctgcag taacccagta atgacggcgt tgagggttga
    16381 acaaatcctg accttgcgaa tattcgctgg caggtgtgct gacatgtagc aggcgttgca
    16441 tcagcgtcgt catcagatcg gtatgatcag tcagagcatt aatacgctgc gccggcgtgc
    16501 ctggccagtg aatcactaat ggcacctgca gatgaccgtg ggaccagtca aaggtttctt
    16561 cgtcttcgct cagcggaata ccccgaccgg cagtgataat caccaccgta ttgtccagtt
    16621 tgccagaatc acgcagtgca ttgagcacgc ggttgatctg gtcatcgaca ttgcctgccg
    16681 cccggctata tttccgtgca aatgcctgct gattgctgtc gtcaatgtta gtgccattga
    16741 aagagaccca cgagaaccag cggttatctt cttgtgcgta gcggcccagc cagttgatcc
    16801 actgcgtggc ggtctgctcg tcggattggg tgcgtacgct cggcatcgag aaatctgaca
    16861 acaatgcctg gcgatacagc gggctggtaa agccatctga tgagaataat cccagctgat
    16921 agccttgctg attaagcgca gtaattaacg ccgcaggggt acgggtcgac agaatgccgt
    16981 ccatatagct cggcgagatg ccatagaaca ggccaaagat gccgttgtct gtagtgttgc
    17041 cggagctcat atggcgcgtg aacgaaatat tttgctcagc aaaacctgcc agcgcaggca
    17101 tctgcttctc gaagcgtgag tagttcaggc catcgacagt aatcaacagc acattctgcc
    17161 cggtgcccat atcgcgatag cgcagttcgc ttaacggata ctgaacggaa acggcgtctg
    17221 gattaccttg ctcaataaga cggcgttgat actcctgcgc atcaagcaga ccatgcttct
    17281 caagaaaacg tcgcgccgtc atcgggtacg aaagcggcag gttagcgcgc tgcatggtga
    17341 tcgggcgata gaagttggca tcggcccaga tatacaccac atgcgaggcg ataaaggcga
    17401 taaataagaa tgcggccagc gggcgcgcga agcgccgacg acgcgtcagg ctgcgcagct
    17461 tttgccagct ccacgtcgca aacaccagtt caagcaataa aataaccggc acgctgatga
    17521 acatcagctg ccagtcgcgc gccatctcat tttcgtctgg gttgataacc agttgccaga
    17581 cgatgggatt aagatggaga tggaaacgag taaagacttc gctatcgatc agtaatagcg
    17641 tcattcccgc cgttgccaga atgacggaca aaaacctcat cagcctctgg gagccgacga
    17701 taaaggtcag cgggaagagg atcagcaagt aggtggcgaa caccaggaag ctgaaatggc
    17761 cgataatgct tacgtaggaa taaatgcgac cagcaagcgt tgtcggccag tcggcgataa
    17821 acaggtaacg gctgccaatg acgagcgaaa gcagaatatt gaacagtgca aaccagtgcc
    17881 cccaactgac catctgggag actttttcac ggtagcgctg acgatgagtt accataacct
    17941 gttatctgtt tcccttagtg cgctttgtct tcgttgatag aggactgtaa ggcgcgggca
    18001 aaagagttgg caattgcctg gcgttgggcc ggggcaatgc tggtgttgat aaggttagtg
    18061 accatgttgc ctaacaccat cagggaaagg tcggtcggag ccttatgttt ttccagtacg
    18121 ttgagcagct ctgcgagcaa ttgttcaacc tgttcatcac tgtagcggga aatttgtggc
    18181 ataaatcgaa atcagttagt tgatgaaagg gcaacatatt accgtagcaa cggttttttt
    18241 tctgcttttt tatgcgttaa atcattctcg ttacgctgcc ttctgtaaaa tgaccgccat
    18301 cttttattgc gtcgcttaac cggcggtggt tgaataccgc ccggtcttaa aggagagttt
    18361 atcatgagtc tggatatcaa ccagattgcc ctgcaccagc ttatcaagcg tgatgagcag
    18421 aaccttgagc tggtgttgcg cgattcattg ctggaaccga cagaaaccgt cgttgagatg
    18481 gtggcagaac tgcatcgggt ctatagcgcc aaaaataaag cttacggctt gtttagcgaa
    18541 gagagtgaac tggcgcagac gctgcgctta cagcgtcagg gcgaagagga ctttctggca
    18601 tttagccgcg cggcaaccgg gcgcctgcgt gacgaattag cgaaatatcc ttttgctgac
    18661 ggtggattcg tgctgttttg ccactaccgt tatctggcgg tggagtatct gctggtggca
    18721 gttctgagca acctgagcag tatgcgcgtt aacgaaaatc tcgacatcaa cccaacccat
    18781 tatcttgata tcaatcatgc ggatattgtt gcgcgtatcg atttaaccga atgggaaacc
    18841 aatccagagt ccacccgcta tctcactttc ctgaaagggc gagtagggcg caaagtggcg
    18901 gatttcttta tggatttcct cggggccagc gaaggattga acgcgaaagc gcaaaaccgt
    18961 gggctgttgc aagcggttga tgatttcact gccgaagcac aactggataa agcagagcga
    19021 cagaacgtgc gccagcaggt ttatagctac tgtaatgagc agctgcaggc aggcgaagag
    19081 atcgaactgg aatcgctgtc gaaagagctg gcaggcgtaa gcgaagtgag cttcacggag
    19141 tttgcggctg aaaaaggcta cgaactggaa gagagcttcc cggcagaccg tagcacactg
    19201 cgtcagttga cgaagtttgc cggtagcggc ggtggcttga ctattaactt tgatgcgatg
    19261 ctgttaggtg aacgcatctt ctgggatcca gcaaccgata ccttgaccat taaaggcacg
    19321 ccgccgaatt tgcgcgacca attgcagcgc cggacgtctg gcggcaatta atcggttttg
    19381 tgttctgaat gccagatgcg gcgtgaacgc cttatctggc ctgcaaaaaa tagcaatacc
    19441 gtaatcaggc ataatctgca gatacaaaaa accccgccgg agcggggttt tttacaactt
    19501 attcagcaat taagcgcgaa cgaagtcgat gtgctgcagc ttcggtttgt acgggtgacg
    19561 ctgtacgtcc tgagctttaa ctttgatttc tttaccgtca acaacgatgg tcagaacttc
    19621 gctgtagaat tcagctttag cttgcatgtt catgactttg tcgtgatcca gctcgatagc
    19681 cagcggcgct tctttgccac cgtagatgat tgccgggaac ttgttagcgg cacgcaggcg
    19741 gcggctcgca cccttaccct gctctttacg tacttctgcg ttgatagtaa acatttcttt
    19801 ctctcttaaa ttaatcctgc tacaggcgac ccagcaacag gtaagtgatc tgctttgcgt
    19861 atgcaaaagc gggcgagatt ctatactcaa tcgctttata catcaatcaa aactaccatt
    19921 aaccgcgtaa ttcgtgcgcc cgacgaaaac gaccttcgta atcgaacact ttttcacgca
    19981 cttgccagta ctgacctttc atgcaggcaa cgacaaaatc cgggtgtcgc aataaggctt
    20041 gctgggcgag gatatcggcg gcggtgatcc agcgcagagg gatgcccggt gtgcgcgtat
    20101 gcgggcggat aaaaagctgc tcgaaggcag ttcgctgggc gggcgtttgc agacggaaac
    20161 gctcactcac atccgcgccg tcttcatcgt aataggtgat tttcaaccat tcgcctttct
    20221 cgtcgtgctc atgctgcaaa gacatgccgc tacagcgtaa taccagcgca tctttcagtc
    20281 gtagcgccgc ttttaacata tcatccggat caaccagtac cgtgtcacat tcgcggcagc
    20341 ggcgagcggc aatatcattt tctgcgttac attgcgggca atttttaaaa cggaaacgga
    20401 agtcacactg ttcgcgatga ccgtcgtcat cttcaaacca tccctgacag cggcgaccaa
    20461 agtgttcaat caatgtcccg tcggcggtcg ttttccccca aaaggtgttg gcaaaaccgc
    20521 aggcagggca gaaaacctgt accggaacgt tgtcactttt gcctttcggt gtaccaactt
    20581 ccggcgcgta gagatcgtga ggattacccg cataatcaag aattaaacaa tcagtcttgc
    20641 ccggagcgag acgcagaccg cgcccgacaa tttgttggta aagactcact gattcggtag
    20701 ggcgcagaat ggcgataaga tcgacgtgcg gggcgtcaaa tccggtggtc agtactgcga
    20761 cgttgaccag atagcgaaaa cgctgggctt taaaatcttc aattaacaca tcgcgctcag
    20821 cgccgggggt gtcgccagta atcagtgcag catcttcggc aggcagtaat cccacaatct
    20881 cttttgcgtg ttcaacggtg gcggcgaaaa tcattacccc tttgcgcttt tcggcgaact
    20941 ccatgatctg gctgatgata tgtggcgtaa tgcgttgctg ttttttcagc tcacggttga
    21001 gatcagcttc gctgaacagc ccgttactct gtgcctgcaa gcggctgaaa tcgtattgca
    21061 ctactggcat atccagtcgt tctggcggcg tcagatagcc gtgtttaatc atataacgca
    21121 gcggcagctc ataaatgcag tcacggaaaa gggctttctc atcgccgcgt accatgccgt
    21181 gataatggaa ctggtagatc cagcctttgc ccaatcgaaa aggcgtggca gtcagcccca
    21241 gcaggcgtaa gtggggattc actttagtca ggtgagtgag gatttgctga tactggctct
    21301 cttcatcgtc accaatacgg tggcattcat cgacaatcaa cagcgaaaat tcaccctgaa
    21361 aggcatcaag attacgagcg accgactgca cgctgccaaa taccacttta ccgtggctct
    21421 ctttgcgctt taacccagcg gcaaaaatat cggcttccag ccccagcgcc tgatactttg
    21481 catggttttg cgccaccagt tctttgacgt gtgccagcac cagaacacga ccacgcgcca
    21541 gccgtgccag ttcggcaatc accaggcttt tacctgcgcc ggtgggcagc acgataacgg
    21601 caggggtttt atgacgacga aaatggttga gcgtggcatc cacggcttct tgctgatatg
    21661 ggcggagtgt aaaaatcatg gtctcgcgta ggtagattat tctgctaata gtatgccatg
    21721 gatctttttc ccttgaggga tgttattaga ccgctatact gggcagacaa cagttcagat
    21781 tttcgggtca atgcccgacc tccgttttta caggcaaaat cacacacatg cgacttgata
    21841 aatttatcgc acagcaactc ggcgttagcc gtgctattgc cgggcgtgaa atccgcggca
    21901 atcgtgtcac cgtcgatggc gaaattgttc gtaatgcagc gttcaaactg cttcctgaac
    21961 atgatgtcgc ttacgatggc aacccgctgg cgcagcaaca tggtccacgt tacttcatgc
    22021 tcaataagcc tcagggctat gtttgctcca cggacgaccc tgatcaccca acggtgctct
    22081 attttctgga tgaaccggta gcctggaaac tgcacgccgc aggacgtctg gatatcgata
    22141 ccaccggtct ggtgctgatg actgatgatg gtcagtggtc gcaccgcatt acttctccgc
    22201 gccatcattg cgagaagacc tatctggtga cactggaatc acctgtagct gacgatacgg
    22261 cagagcaatt tgctaaaggc gtgcagctgc ataacgaaaa agatctcact aagcctgcgg
    22321 tgctggaagt gattacccca acgcaggttc gtctgaccat cagcgaaggg cgttatcatc
    22381 aggtgaaacg catgttcgcc gctgtgggta accatgtggt tgagctgcat cgtgaacgta
    22441 ttggcggtat tacgctggat gctgatttag cccccggtga atatcgtccg ttaactgaag
    22501 aagaaattgc cagcgtcgtc taacttttcc atgactttca ggagcccgtt gtgaccaccc
    22561 gacagcattc gtcgtttgct attgttttta tccttggcct gctggccatg ttgatgccgc
    22621 tgtcgattga tatgtatctg cccgcgctac cggtaatttc agcgcagttt ggcgtacctg
    22681 cgggcagtac gcagatgacc ctcagtactt atattctggg ctttgcgttg gggcagttaa
    22741 tctacgggcc gatggcagac agctttgggc gtaagccggt ggttcttggc ggtacgctgg
    22801 tgtttgccgc cgccgcggtg gcgtgtgcgt tggcacaaac catcgatcag ctgattgtga
    22861 tgcgtttctt ccacgggctg gcggcggctg cggccagcgt ggttatcaac gctttgatgc
    22921 gtgatatcta cccgaaagaa gagttctcgc ggatgatgtc gtttgtcatg ctggtgacga
    22981 ccattgcacc gctgatggca ccgatagttg gcggctgggt gctggtgtgg ctgagctggc
    23041 attacatctt ctggatcctc gccgtggcgg cgattctggc ttcggcaatg atcttctttc
    23101 tgatcaaaga aaccttacca ccggagcgtc gccagccttt tcacattcgt accactattg
    23161 gtaactttgc ggcgctgttc cgtcataagc gtgtcctgag ttatatgctt gccagtggtt
    23221 tcagctttgc cgggatgttc tcgttcttaa gcgccggacc gtttgtctat attgaaatta
    23281 accacgtcgc gccggaaaac tttggttatt actttgcact aaacattgtt ttcctgttcg
    23341 tgatgaccat ttttaacagc cgcttcgtcc gccgtattgg cgcgttaaat atgttccgct
    23401 cggggttgtg gatacagttt attatggcgg cgtggatggt catcagtgcg ctgctggggc
    23461 tgggattctg gtcgctggtg gttggcgttg cggcgtttgt gggctgcgtg tcgatggtgt
    23521 catccaatgc gatggcggtc attcttgatg agtttcccca tatggcggga acggcatctt
    23581 cgctggcagg aaccttccgt tttggcatag gggcaattgt tggcgcattg ctttctcttg
    23641 cgacctttaa ctctgcatgg ccgatgattt ggtcaattgc attctgcgca agcagctcca
    23701 ttctcttctg tctgtacgcc agtcgaccga aaaaacggtg atctattgca caacgaggaa
    23761 gctaaaaggc ttcctttgtt gatgcatgtc aaccacaaat ctatcattcc cccgatatat
    23821 gtttatttta tgtaaaatca atttatgtaa aaagtcacat cattgtagtt aaaaaggttg
    23881 agttagatcg cagaaacggg tacatatagc cccgcaaacg tgaccacgcc cgcagatatt
    23941 acttaaatca gagccataga ggccacgcag gcgaggcatc aatctttacg atctgtataa
    24001 agacggattg ttgatgatgt gttaaaattg atgtaaacaa attgtgaagt gaatgtgctt
    24061 ccggggaaaa taagtgactt cattacaact ctcaatcgtc catcgactgc cgcagaacta
    24121 tcgctggtct gctggtttcg caggttcgaa ggttgaaccg attccgcaaa atggaccgtg
    24181 cggtgacaac agcctggtgg cgcttaaatt gcttagcccg gatggtgata atgcatggtc
    24241 ggtgatgtat aaactaagcc aggcgttaag cgacatcgaa gttccatgtt cggtgctgga
    24301 gtgtgaaggt gagccgtgcc tgtttgtaaa tcgccaggac gagtttgctg caacatgccg
    24361 attgaaaaat tttggtgtgg caattgccga acccttttca aactacaatc ctttttaagc
    24421 gtcagctcaa tgccagtagc tgacgcgtat actcctgctg cggtgtggca aacacgcgcg
    24481 cgcacggtcc ttgttccact acttcccctt gtcgcagtac gataacctga tgacataacg
    24541 cgcggacaac gtgcaaatcg tggctgataa acaaataggc cagttgatgc ttttgttgca
    24601 atgatttcaa tagcgtcaat atttgcgcct gtaccgtttt gtcgagtgat gatgtcggtt
    24661 catcaagtat gatcagcgag ggcttaagaa ttaatgccct ggcaatcgca atacgttgtc
    24721 gctgaccacc agagaactcc gccggataac ggtggcgtgt ttcaggatct aatcccactt
    24781 catgcatcac ggctatcact tgttgttcgc gttgtgcggc agaaagcgtc ggctggtgaa
    24841 cccgtaagcc ttcctcaata atctgcaaaa cgttgagtcg tgggttgagc gaggagtttg
    24901 gatcctgaaa taccacctga atgcgatgac gaataggtaa cagctggcgg cgatttaaat
    24961 tttgcagtgg ctgaccgtca aagatgatgc tgccctgaga attaatcagt cgcagcagcg
    25021 ccagtcccgt cgtacttttc ccggaaccgg actcgcccac taaacccagt gtttcacccg
    25081 ctcgtagcgt aaaactgatg tttttcacca ccacattatg atccacaatg cgcttcaaaa
    25141 tccctttgcg aatggggaag gcaacctgaa gctgttcaac atccagcaac gttgaggcag
    25201 gttctggcaa cggcactgga tcgcctgacg gttcactgtt gagtagcttt tgtgtgtaag
    25261 gatgagtggg tgaggcaaat agcgtagcgg cgtaattttg ctcgacacag cgaccgtttt
    25321 gcattaccgc cacgcggtgg gccagttttc tgacaatgct gaggttatga gtaataaaca
    25381 gcatgcccat attcagctcg ccttgcagtt cgcgcaacag ctgtaaaatc tgcgcctgga
    25441 cagagacgtc cagtgcggtg gtcggttcat cggcaattaa taattccggt cgcgttaaca
    25501 gcgccatcgc aatcatcacc cgctggcgtt cgccgccgga gagctgatgc ggataatctg
    25561 tcagccgttt tgccgcctgg cggataccaa cgcgatcaag gcagttaaga atttcgcctc
    25621 gagccgcttc ccgacgcatc ccgcggtgga gtgaaagcac ttcataaagc tgtttttcca
    25681 gggtatgcaa tggatttaac gacaccatcg gctcttgaaa aatcatggcg atcttattac
    25741 cgcgtacacc gcgcaacgtt tgatcgctgg catgaagcag cgattcgcca cgaaaacgaa
    25801 tatcgccgga gagatattca accggcgggg aagggagcag gcgtaaaatt gacagcgcgg
    25861 taacgctttt gcctgaagct gactcaccca ccagcgccag cgtttcgcca gcctcaatct
    25921 gtagtgaaac atcattgact actgtacgta cggtttgctg atggcgaaaa cccaccgaca
    25981 aatcttcaat cgctaacaga gtttgcgtca tgctacaccg ccttattagg atcaaatgcg
    26041 tcgcggacgg cttcaccaat aaagatcagc aaagacaata atatcgccac cgacaagaag
    26101 gcggtgatcc caagccacgg agcctgaagg ttatttttcc cttgtaacag cagttcaccg
    26161 agtgacggtg aaccgagcgg cagaccgaag ccgaggaaat cgagtgaggt cagggtggtt
    26221 atcgaactac ataaaataaa cggtaaaaag gtgagggtcg cgaccatggc attaggcaac
    26281 atatgacgca ggatgatact gcgatcgctg acgccaagcg cctgcgccgc acgaatgtag
    26341 tcgaaattac gggtacgtaa aaactccgcc cgcaccacgc cgaccagact catccagcca
    26401 aacaagacag taattgccag cagccaccag aagttaggct gtacgacgct ggaaagtaaa
    26461 ataatcaaaa acagcgtcgg catccccgac catacttcaa taaagcgttg cccccagaga
    26521 tcgactttac cgccgtaata gccttgtagc gcccccgcca gcacgcccat cacgctggaa
    26581 cagagagtca gcatcaggcc aaacagaacc gagatccgcg tgccatagag aatacgtgcc
    26641 agcacatcgc cgccgttggc atccgttccc agccagtttt gccgggaggg tggagaaggg
    26701 aagggcttat cggtagcaaa gttgatactg gtagcaccaa agcgaatcgg tgcccacagt
    26761 acccagccgt tattttccag ccgttgtttc agccacgggt cctgataatc agcctgactt
    26821 gccagcgggc cgccaaaatc gctttcgctg tagtttttca acaacgggaa ataccaactg
    26881 ccgtcataac gtaccagcaa cggtttatcg ttggcgataa gttcagaaca caaactcaaa
    26941 ccaaacaaaa cgaggaaaat ccataacgac cagtagccgc gacggttatg gcgaaaacgc
    27001 gcccaacggg cctgattgac ggggctgagt cgcgacatta acgtccctca aaatctatac
    27061 gcggatcgac cagcgtatag ctgatatcac tgacaatatt cagcagcagg ccaatcaggg
    27121 tgaaaatata caaggtacca aacattacag gataatcgcg cgagacggtc gcttcgtagc
    27181 ccagtaagcc cagaccattg agtgaaaaca tcacctcaat cagcagcgag ccggtaaaaa
    27241 acatgctgat aaacgtcgcc ggaaaaccgg caatcaccag cagcatggcg ttgcggaaca
    27301 catgtttcca gagaatattt ttttcactta cccctttcgc acgcgcggtc accacgtatt
    27361 gcttgcgcac ttcatcaagg aatgagtttt ttgtcagcat ggtcagcgcc gcaaagccac
    27421 caatcactgt cgccagcacc ggcagcgtga tatgccacag ataatcggtg attttctgat
    27481 accacggcag cgaatcaaag ttagcggaaa ccaggccgcg tagagggaac aggtcgaaat
    27541 agctgccgcc cgcgaagaag acaatcagca ggatggcaaa caaaaaggcc ggaatggcgt
    27601 agccgatgat gataaatgcg ctactccaga cgtcaaagcg gctcccatta taaacagctt
    27661 tgcgaatgcc taacggaatc gacaccagat agataatcag cgtgctccac aatccgaggg
    27721 tgatggaaac cggcagactg tctttaatca gcgtcagcac cgaggcgctg cgaaacaggc
    27781 tatcgccaaa atcaaagcgg atgtagtccc agagcatttt gaagtaacgt tcgtggatcg
    27841 gcttatcaaa accgtagcga tgagtgatct cagcgatcac ttctggatct aatccgcgtc
    27901 cgccacggta cttactgtcg ctgatattgc cgacacccgt ttgcgcatgg ctggcacgaa
    27961 caccttcacc gcctgcgccg ggtaatactc cggcattacc aaactcaatg gcggcgatgg
    28021 cctggtcgac cggaccgcca ggcgcaattt gcacgatgaa aaagttgatg gtgataatcg
    28081 cccataatgt tgggatcacc agcaacagac ggcgaatcag gtaagcgccc atctactctc
    28141 cctgtttgct ggcggacggc agtttggccg ctttattgac gtcataccac caggtatcga
    28201 taccgaggct atagatgggg cgcacggccg gctgggagaa tttatcccac caggcgagac
    28261 ggtcttccgc catgtaccac attggcagca tgtaataatt ccacgttaat acgcgatcca
    28321 gtgctcgccc caacggcagt aatttttctt tatttccctg cgcggcaata atttggttga
    28381 tcagcgagtc gataaccggg ctttgcacgc cgggggcatt ataagtggaa ttgatatatt
    28441 ccgatgacca ggaaatctgt aaatcggaac tgggccacgg catcgcccgc cataggcgcg
    28501 gcatcatgtc atagtcgcga ctgcgcatgc ggttagtgat ttgcgagtta tccaccttgc
    28561 gaatgtccat attgataccc agccgttgca ggctgtgctg gaacggcaat acccactgac
    28621 tattgctgct tgcgggaagc aataattcaa agctgagcgg ctgacccgtt gtggcattaa
    28681 cgcgttgctg acccttcagc acccagcccg cttcgttgag aagtttgtcg gcttttaaca
    28741 ggttgtcacg atcgtagcca tcgcctttag ataccggcgg ctggtagatt tgtgtgaaga
    28801 cttctggcgg tagatctttt ttcattggtg ccagaagcac cagctccgcg gcgtcggggt
    28861 aatttctggc cgcgtattcg gtattctgaa aataactgtt cgtgcgactc caggcgttgt
    28921 aaaacaacgc cttgttcatc cattcaaagt caaaggcgag agtgattgct tcccggaccc
    28981 ggcgatcgct gaataccgga cgttggatat taaacgccag ccagcgcgta tcctgggctg
    29041 attcgttctt ttgctcatct ttgatgatgt attttttatc gaaattttta ccggtataac
    29101 gcgtggccca gtttttggcg tcgttttcca tacgcaaatc aaaggcacct gctttaaacg
    29161 cttcaaaggc gacattatca tcgaggtaat aatcgtagcg aatggtgtcg aaattccagc
    29221 gtccgcggtt taccggtaag tttgctgccc agtaatcttt cacgcgggaa tagacaatat
    29281 tttgccccat tttccaggac gtaatgcggt acggaccact ggcaagcgga ggcgtggcga
    29341 gcgggtcgct aagtttgtga tccttccagt acttttctgg aaataccggc agcgaaaaca
    29401 gactcaacat atcttctttg ccgggtttag ctaactcaat gcgcacggtt aacggcgcaa
    29461 tggctttgac ggtggtgcct ttgtagacca gacgaaattg cggcacgcct tcggtcataa
    29521 atttttgaaa agtaaactct acatcgcggg cagtaatggg cgaaccatca tgaaaacggg
    29581 cgcgcggatt aatagcgacc tccacccagg aataatcgtc agcatagcgt gcgctttcag
    29641 caatcagcgg gtaataactg cctggttcgt catcggaagt cgtaaatagc gtgtcgtaca
    29701 gctgctcggt gcgtgcgccc gggttgccgc gcagtgcata gcggttgaaa ttatcgaagg
    29761 tgccgagggc tgacaacgtt atctgcccac cttttggcgc ggcggggttc acataatcaa
    29821 aatggttgaa attaaacgcg taccggggtt cgcccagcac ggcaaaggca tagctttcct
    29881 tgatagcctg cgcctgcaca ccaaaggtga acagagcgat aaacagcagc agtatgcgca
    29941 caatcatctg caacggattt ccttttactc atcattagcc gcaatccggc atgctttcac
    30001 tatgaataat aagggacgta agcacacctt accactgcgg cgtatacggt ttcttcagcc
    30061 agcggacaaa atcgtccagc ggtaacgggc gactaatcca gtagccttgc atgaaattaa
    30121 cgccgcgttc gcttagccat cgcgcttgtt ccggcgtttc gaccccttca gcaactgtca
    30181 gcatattgag acgtttcgcc agcgtcagca ccgcgtcaag tacgggtgaa gtgatcgttt
    30241 ccgtaccgat ggtgttgata aatccacggt caattttcag ataatcgagc gtaaaacgct
    30301 caagatagat aagcgcgctg tgcccggtgc cgaagtcatc aatagcaatt tctacgccga
    30361 ccgagtgcag ccaggcgaag agttgtgtgg cttcttgctc tttcagcata tcgcgctcgg
    30421 taatttccag cacaatctgg aaatggtgtg cgggtaggga agtgagcagt ttctggatat
    30481 ctgctttaaa gctttcgctg tgcagatggt ccggcgcaat gttaatacca aatttgacgc
    30541 ctactggcag cactttttct aattctgcgg catcgcgggc aattaactca aacaggtgct
    30601 gagtcagcgg cacaatcatc ttttgcgatt cggcaaagtt aatgaaggca tccgggggaa
    30661 tttctcccgc gacaggatgc cgccagcgta gcagtacttc caggcccgtt actcgcaaag
    30721 cttgtgtatc caccaccggt tgatacgcca cgtaaaattg ttcgcgcttg atggcggtca
    30781 tgatttctct gccggggcgc atacgtacgc tcatcaggta atagcagagc aggccaacga
    30841 cagttccact catgccgccc agtaaaaatg cgtaccacac atcgttccat gtccagtcat
    30901 ctgcataaag ccgaacggtc agaggaatgc ccgcaatttt agtttcacgg actggcatgt
    30961 cggttaattc gttaacgttc atcaaacgtg aagaaaaggt agatagcgca gtattaccaa
    31021 taatgagggc gacgccatcg taatcttcct gccgtgaact ataaaagaga gaaggcgtca
    31081 ggttgagatt cagagcggca aagacgccgc tatttttcag caaagggttg cgataccaga
    31141 tgacgattgc gggtttgttc ggcaccatcg gcgtgccggg taagatcgcc atatcgacgt
    31201 ttttattaat gtcgagcgcc ggaatcaatt cattgagtgg aatgtccatc tcaccggtcg
    31261 cagatgaaca gaatgttttt ttatctttca ccagcacaaa cgtacggaca ttcatgctaa
    31321 acgccgcgcg ggcggtcagt tcggggttag cttgctggca ggtatctaag gtcagcggct
    31381 ggagccggtc agtagtggat ttcagatcgg caaaataggt atcgagatac ttttgtacgt
    31441 cggtaatcag tgtgtcgtat ttgacttcgt gcttatgcca ggccactaaa aattgaaggc
    31501 aactcaccag tatcgcaatc atcacgcctg caacaatgca ggtaagcagg agcttacgtc
    31561 cagaattggg agcgcgtatg aacatgattc cgttaatttc ctgaaattac gacaacattt
    31621 aaaagataac aatgaccgga ctatagcctg gatcaagacg cggaaacatc attttctctg
    31681 atgaatgaaa acaaaaaagc actgcctaag cagtgctttc agttaactcg tcaggatagc
    31741 caagggattg catccaaacg gtttattagc tgcggctgag aacccggcgt gcttcgttgt
    31801 aacgcttctt ccagtacggt tcattcatgc tggaaataat aacaccactg ctggtggaag
    31861 catggacaaa ctgattgttg ccgatataaa taccgacatg gcgtcccgtt gaaccggcac
    31921 ggaacagaac taaatcaccc gtacgcaaat tactgcggga aacagattta cccatttcct
    31981 gctgttcgta agtcgaacgc ggaagttcta agccaaattg ctcacggaat gtacgctgta
    32041 cgaaaccaga acaatcgata ccttttttag tgctgccgcc cagacgataa cgtacgcctt
    32101 tccagtcagc atactgatcc ataattcgcg attttacgtc gacattacga accaggtttt
    32161 caaattcatc ctgagaagct tgcagtgatg atgtttcact acccactgca cgtgtctcag
    32221 gatgcatatt ctttgcggtg ttatttgcac tacatgcaga aagcagaacc gctactgcaa
    32281 tcgcgggaat cccgcgcaag atatatctca aaatcggttg agatttgacc atgttgtttg
    32341 ttttcccttg aagtccttaa cgacaaatat cgttataaaa aaatgccaag cgacacgaga
    32401 ctaattacct gggcatgttg aaacaattcc ttaaggccaa atctgtggac taacgcacaa
    32461 aattatatgc tgtgaggata atctaccgcc gaggcaaaac gagttcgaga ttacccgatt
    32521 ggtgcggtct tggcgagcgg ttttacatga ttttttataa gaaaaagtga tacagaaagt
    32581 taataagcgg gattggtcaa ttaaagtacg attgagtgaa acgcatgcaa tccattcatt
    32641 tacttagaga atgatttttg cgcgcgatgg atgaaagcac ggtgcgcatc atcaggatta
    32701 tcctcactat aaaaataagc ctgatgatgt taattactgt gagttatttg ttttggaaat
    32761 gtttgttttt tcccggtagt gatttgtcaa aaaaggtgat taatcgatca cttaatggcg
    32821 tcagcaacac ccagggcaaa ccgatcaata tcacggtcat cgaaccgaca atgatgtcag
    32881 taaaccagtg tgcgccaatc attactctgg gaaatgcaaa aaccacaaaa ataataaggg
    32941 cgataaggcc tgcaactttg ccgaaataac gccacatgaa tgccgaaaaa ataagcagca
    33001 tcatgccgtg atcgccggga aagctatctc gtgaggcatc tttcgtggga acagagagca
    33061 gttcgctgac gcggttaata tcggtaaaag tcaatgttgg gctggcccgt tttacaggaa
    33121 ttaatgcctg acccagatgg tttaatacca ctgcagttaa tagcatgacc agacccataa
    33181 tcacgatacg tcgtctgcca ggggcgtttt ctttcagcca gaaactcagc atcaacattc
    33241 ccatcgccag cagagaacaa ccgtcgaaag cgcgattgtt ggtcaatgca accagccaca
    33301 aaaaggcctt actttcgacc agtttctgat taaagaaata aaaaatatcc gcatcaattg
    33361 gcaaccagaa tccatgatta acgggaatat accaggaaag aaacagcgcg aggccgacaa
    33421 tattcaacaa cactatttgc ggcaaatttt taatcatatt ttctctaatt atcttactga
    33481 aaaacgcagg caaccttacg cggtagtcgc taaacgaagc ttcaacagcg cgctctgcaa
    33541 agcattccag tcagcttcgc tgctggaaat cagctcaata cggctgtccg gtggcgcaac
    33601 gttttgcgtt tcaatgtgca ggtcatcgcc ctgacggttg attcgtacca gcccttcggg
    33661 aatacgcagc acgcctttga cgcgttccac cggtgcaagt cgcgcccatt ccagaatgcc
    33721 aatggtgtcg aataccgtat cagcgtcgaa tatccagccg caggcctgat atccttgccc
    33781 gctgttcaga ctgcgacgcc agcgttgatg ctctggcagg cttaacgctg ctaacccttt
    33841 tttcacgaca tgctgatgag aatgcgcggc gctggcgggc aactcggcta aattacgacg
    33901 cggcaaatcc agaagatgac cgtcaacttt cccatgctca ctgtgaatta attgtcgatc
    33961 gccaccattt tgctgccacc aacgctgtag cgcttgctca ctttcgggcg tcgcacggtc
    34021 ggatttattg gcgacaatga tgtctgcggc agccagctgg tcacggaagt tttcattgct
    34081 ggcgcttttt tcgtccagca gcaagcgcgg atcgagaatg cacaaggtgg cgcgcagatc
    34141 tatccacggt tcatagactg gtgcggttaa cagatcgagg atctgtttcg gatggcccag
    34201 cccggtcggc tctatcaaca agcggtctgg ttttccctga cgcagtaagg tattcaaccc
    34261 tacctgcatg ggtaaaccat taacgcagca catgcagccg ccggggatct ctttcagcaa
    34321 tgcgccgcta tcggcgagca aagcaccatc aattccgact tccccaaatt cattaaccag
    34381 taccgcccat ttttcgttgg gatctttatg ggctaacaga tgaagaatcg acgtggtttt
    34441 cccgctgccg agaaaaccgg tgatgaggtt ggtcctggtc acaacggctc cgcaatactc
    34501 tggtaagtta actgagcgtt aatcctggct aaaaaaggca gaaaagagaa ggggagagcg
    34561 gacgaaggtg agcttcatcc gctttaaccg cagaaatcgt taaattttga gggtgtttaa
    34621 tagcgcctga tgtgcgccga attgtacgat ttgttgccag gcttgttcga tggcctgcac
    34681 aaaatgcggg ttatctggca gatcatcacc gaaaacttca cgcagggaaa gcagggcggt
    34741 tacgcgttgt tcactgctgc tgcccgcaac aagttcgcga attttatcgc taagcggatc
    34801 gcgaacatca atggcatttc cggcatcatc aacgccgctg acgtaacgca tccagccagc
    34861 aacgcctaat gccagcaacg accagtccgt ttcacgcccc agatgtatgc gaatacctgc
    34921 cagcatgcgt tgcggtaatt tctggctgcc atccatcgcg atttgccagg tcttatgttt
    34981 cagcgacgga ttagcaaaac gtgcaattaa cttatccgca tattgtgtta aatcgacatc
    35041 tttaatttgc agtgtcggcg cttgctcatc cagcattaat gttctggcag catggcgaaa
    35101 tacgcgatcc tgcatgcaat cactgatatg ggcgaatcct gagaggtaac ccagataagc
    35161 gagaaaagag tggctgccat taagcatacg cagtttcatc tcttcccatg gcaggacatc
    35221 attcaccatt tgtacacctg cgacttccca ggcaggacgc ccagcgatga agttatcttc
    35281 aaccacccac tggataaacg gttcgcagct aatcgcgcag ggatcattta cccccagatg
    35341 ctggctgatt tctgccagtg attcgtcggt tgcagccgga acaatgcggt cgaccatggt
    35401 tcccggaaaa ctgacgtgtt cttttatcca cccggcgagt tctggcgaac gtttttctgc
    35461 cattcccagc accgcgtttt tcaccacatg gccattgtcg ggaatattgt cgcaggagag
    35521 cacggtaaac ggtgtaaggc cgcgctcacg gcggcgtttc agtgcttcga cgagaatacc
    35581 cggtgcggag tgaggttctt caggggtttg tagatcgtga ataatccgcg gattactggt
    35641 gtcgagtgca ccggtggccg ggtcaataca atagcctttt tcggtaatcg tcagggaaac
    35701 aattgccacc tgtggctcgc aaaatttctc aataattgcc gctaaggaat ccagttttgc
    35761 attaaggcat tcgtgaacgg caccgacaat tatcacctga ttgccgtccg cacctttttc
    35821 cagcacggta tataaatggt tctgtgcgcg gagctggctc atcagttgat caccgctgaa
    35881 caagctgatt tcacagatcc cccagtcgcc gccctgggca ttcagcacac gatcggtcag
    35941 taacgcctga tgagcgcggt gaaaggcgcc aaagccaaaa tgaacgatac gtgattgcaa
    36001 ctgctggcga tcgtagcgtg gagcgtgtac atgatgcggg agcgtaacgg aggcaattgt
    36061 cttcatagga gtcacgtctt agtaacgata aattaacaat gacagtgtaa agttatatga
    36121 cagcaattga aattggtctg gattgttaat gcgatgaagt gtgagggcga tcaatcaacg
    36181 agtgaaaatt gagggcgtaa ggtgttcagg aagcgcaaaa tagaagattg gtataataaa
    36241 ttaaaaaagg gacagcctga gctatccctt ttctgcatgc ggctgaagtt agtcagcacg
    36301 ccccatataa cggcgttctt cgatatggat acgaattttt tcgcccgggc ttaagtattc
    36361 tggtacctga atcaccagac cagtgctcaa tgtcgccggt ttgttacggg cgctggcaga
    36421 tgcccctttg atgcctggtg cggtttcaac gatttccaga tcaacggtct gcggcagctc
    36481 aagcgccagc agttgaccgt cccaggtcag cacctgcatg tccggcatgc cgccctctgg
    36541 cataaacagc aactcttctt caatctgatc tttggtgaag gtatacgggg tatagtcttc
    36601 tttatccata aagacatatt cgttgccatc gacataggag aagtcaacgt aacggcgggt
    36661 cagcgtcacg gtgtcaacga tatcatcacc tttgaaacgc tcttctactt tcagcccggt
    36721 acggacatca gaaaaacgca ttttgtacag cgtagcggca ccgcgggcag tgggcgactg
    36781 aatatcaata tccttaacca gcagcagttt gccgttgtaa ttcagtacca tacctttttt
    36841 aatttcgttc gctcttggca tcgaaaaatt cctgtagtcg aaaatgtcaa aaatatcgcg
    36901 acaaagtact cgcgacgggc ctttcgggca agtggtattc gcacttttgc tggtgcaaaa
    36961 aaggtggtac tgtgcgcgct catcaatccg gtggttaacc ttaagagaac aacgtatgaa
    37021 atgccgtccg ggttgcgggg cgtgttgcac tgccccttca atttccagcc ctattcccgg
    37081 tatgccagat ggcaagcccg ccaatacgcc ctgcattcag cttgatgaac agcaacgctg
    37141 taaaattttc acctcgccac tgcgaccgaa agtctgtgca ggcttacaag ccagcgctga
    37201 aatgtgcggt aactcgcgtc agcaggccat gacctggctt atcgatctgg aaatgctgac
    37261 cgcgccttaa acatctttaa tccgcagtaa gcaaaacaga gtggcgataa tcatcaccat
    37321 cgcaaaccag aacacagcgt gataattcca gatctcggcg acgatgcccg ccactgatcc
    37381 tgcgatgatc cagcccacgc gcgacgtgtt ggtatagagc gtggtggctg aacccgcctg
    37441 accgggcatc agatcctgaa aatagagcat cccgatgccg cccagaatgc caataaaaat
    37501 agcatttagc agctgcaagc ccaacagaat gacaggtgaa tgtgccatca gcattcctgc
    37561 gtaaaaacag acgccaccca cggcagcaac gcgcattaag aaacgcttac ccagacgttt
    37621 ggcgaaatat ccggcaatca acatcgtcgg gatttccagc ccggcggcgg tccccatcat
    37681 cacaccggcc agtttctcgg gaagatgcag ttcgttgata ataaatagcg gcatgttgat
    37741 gatgtacagg ctgttcgagc cccacatcaa tgtacaaatg acaaacagca gcagcgtatc
    37801 gcgacggtta cggcgcggcg cttcgatcgt gccggtcgcc agcggaagct ctttttgcat
    37861 cgacggtaaa aacagccaca ccatcacacc gcaaacaata aacgctaccg ctgcgctcag
    37921 atacattacc gtaaagctga aacccatcgc taaggcataa gccagcggtg ggccaatgac
    37981 ccatgccagt gaaacctgag cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt
    38041 tttgtcggca tgttcacggg caagggcaaa catttgcggg ttagcggtcg agccaaagct
    38101 gctaagaaag acgccaacga atagcaaaac aaagtagttg cgattccagg caaaaagggt
    38161 gcaggccagc acgcctaaca ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt
    38221 atcagagcgc ccggcgagaa actgacttac cagaatccca atgacagcgc tgccggtaaa
    38281 gaagaatccc accatcgccg gacgagcatg tacttcatca gtaagaaaaa tactgagtgt
    38341 cggggtttgc agagcgcccg caataccggt gagaaaggca acgattaaaa acgccgtcga
    38401 ggtcaggtca aacgatttcg cgctggagac tgcgggggag ttatgcatgc tggtcgatca
    38461 ctggaagagg aagacgcgga gtttacgctg cttgtcagaa aataaacaga cagcgaaatc
    38521 aaaagcgcca cgaataatca aaatgccatt tcactgttgc tgattggctg ctgacgctgc
    38581 tgaaatgaaa ttgctgataa cggattttcc catcagcaat taggaaaaat ggcaaaaaat
    38641 tgtgcagcac atcaaacttt tgctcataac tttacggctt tccttgcgtg ctgaaacaga
    38701 agtattatgc tttcttgaaa cgtttcagcg cgatcttgtc tttaacccta agccaggttg
    38761 gcgctttttt tctcatagag gctgaatcgt ttcaattcag caagagagga gaactatgtt
    38821 ccagttatcc gtacaggaca tccatccggg cgaaaaggcc ggagacaaag aagaggcgat
    38881 tcgccaggtc gctgcggcgc tggtgcaggc cggtaatgta gcagaaggct acgtcaatgg
    38941 catgctggcg cgcgaacagc aaacctcaac gttcctcggc aatggtattg ctattccaca
    39001 cggcactacc gacacccgcg atcaggtgct gaaaaccggc gttcaggtat ttcagttccc
    39061 ggaaggcgtc acctggggtg acggtcaggt agcgtacgtg gcaatcggta ttgctgccag
    39121 ctcggatgaa catctgggcc tgctacgcca gctgacccac gtactgagcg atgattccgt
    39181 tgctgaacaa ctgaagtcag caacaacagc agaagaactt cgcgcattac tgatgggcga
    39241 aaagcagagt gagcagctga agctcgacaa cgaaatgctg acgctggata tcgtcgccag
    39301 cgatctgctg actcttcagg cgctgaacgc tgcgcgtctg aaagaggcgg gggcagttga
    39361 cgccactttc gtcaccaaag ccatcaatga acaaccgctg aatctcggac agggtatctg
    39421 gctgagcgat agcgccgaag gcaatctgcg tagcgcgatt gcggtaagcc gtgcggcaaa
    39481 tgcttttgat gtggacggcg aaacggcagc catgctggtg agtgtggcga tgaatgacga
    39541 tcagcccatc gcggttctta agcgtctcgc tgatttattg ctcgacaata aagctgaccg
    39601 cttgctgaaa gcggatgcgg caacgttgct ggcgctgctg accagcgatg atgcgccgac
    39661 cgacgacgtg ttaagcgcgg agtttgtggt gcgcaatgaa cacggcctgc atgctcgtcc
    39721 aggtaccatg ctggtcaata ccattaaaca atttaacagt gatattaccg tgacaaacct
    39781 tgatggtacc ggcaaaccgg caaacggacg tagtctgatg aaagttgtgg cacttggcgt
    39841 taagaaagat catcgcctac gctttaccgc ccagggtgca gatgctgaac aggcgctgaa
    39901 agccattggc gacgctatcg ctgctggtct tggggagggc gcataatgag cagacgtgtt
    39961 gctactatca cccttaatcc ggcttatgac cttgttggtt tctgcccgga aattgaacgc
    40021 ggcgaagtga acctagtgaa aaccaccggt ctgcatgcgg cgggtaaagg catcaacgtg
    40081 gccaaagtat taaaagacct gggaattgat gtcaccgttg gcggcttcct gggtaaagac
    40141 aatcaggatg gttttcagca actgttcagc gagctgggca ttgccaaccg tttccaggtt
    40201 gtacaggggc gcacccgaat taacgttaag ctgacggaaa aagacggcga agtgaccgac
    40261 ttcaactttt cgggttttga agtcaccccc gccgactggg aacgctttgt gactgattct
    40321 ctgagctggc tcggtcagtt cgatatggtc tgtgtcagcg gaagcttacc gtcaggcgtc
    40381 agcccggaag cgttcaccga ctggatgact cgcctgcgta gtcagtgtcc ttgcattatc
    40441 tttgatagta gccgtgaagc gttagtagca ggtttgaaag cggcaccgtg gctggtgaaa
    40501 cctaaccgcc gcgagttgga aatctgggca ggccgtaaac tgcctgaaat gaaagatgtg
    40561 attgaagctg cacatgcgct acgtgaacaa ggtattgcgc atgttgttat ttcactgggt
    40621 gccgaaggcg cgctttgggt taatgcctcc ggcgaatgga tcgccaaacc accgtcagtc
    40681 gatgtcgtaa gcaccgttgg cgcaggggat tctatggttg gtggcctgat ttatggcttg
    40741 ctgatgcgtg aatccagtga acacacactg cgtctggcga cagctgttgc agccctggcg
    40801 gtaagtcaaa gcaatgtggg tattaccgat cgtccgcagt tggccgcaat gatggcgcgc
    40861 gtcgacttac aaccttttaa ctgacagcag gagaggcata atgaaaacgc tgctgattat
    40921 tgacgctaat ctcggtcagg cacgcgccta tatggcgaag accctgctgg gcgcggcggc
    40981 gcgaaaagca aaactggaaa tcatcgacaa tccgaacgac gcagaaatgg cgattgttct
    41041 cggtgattcc atcccgaatg acagcgcgct gaacggtaaa aatgtctggc tgggtgatat
    41101 ttcccgggca gttgcgcacc ctgagctgtt cctgagtgaa gccaaaggcc atgcgaaacc
    41161 ttacactgcg ccggtcgctg cgacagcacc ggttgctgcc agcggtccga aacgcgtagt
    41221 tgcggtgact gcttgcccga ctggcgtagc acacaccttt atggcggctg aagccattga
    41281 aaccgaagcg aaaaaacgtg gctggtgggt gaaagttgaa acccgtggtt ctgttggcgc
    41341 gggcaatgca atcacccctg aagaagtcgc agcagcggat ctggtgattg tggcggcaga
    41401 tatcgaagtg gatctggcga aatttgctgg taaaccgatg tatcgcacct ctaccggtct
    41461 ggcgctgaag aaaactgcgc aggaactgga taaagcggtt gctgaagcaa cgccgtatga
    41521 accagcgggc aaagctcaaa cagcgaccac tgaaggtaag aaagagagtg caggcgctta
    41581 ccgtcacttg ctgacgggcg tctcttacat gctgccgatg gttgttgcag gtggtctgtg
    41641 tatcgcgctt tcttttgctt ttggtatcga agcgtttaaa gagccgggta cgttggcagc
    41701 ggcgctgatg cagattggtg gtggttcagc ctttgcgctg atggtgccgg tactggcagg
    41761 ttatattgcc ttctccattg ccgatcgtcc gggcctcaca ccgggtctga ttggcggtat
    41821 gctggcggtc agcaccggtt ctggctttat tggcggtata attgctggct tcctggctgg
    41881 ttacattgcg aagttaatca gtacgcaatt gaaactgcca cagagtatgg aggcgctgaa
    41941 accgatcctg atcattcctc taatttccag tctggtggtc ggtctggcga tgctctacct
    42001 aatcggtaaa ccggttgctg gcattctcga tgggttgact cactggctgc agaccatggg
    42061 gactgcgaat gcggttctgc tgggggcgat cctcggtggc atgatgtgta ctgacatggg
    42121 cggtccggta aacaaagcag cgtacgcatt cggtgtgggt ctgctgagta ctcaaaccta
    42181 tggcccgatg gcggcgatta tggcggcagg tatggtgcca ccgctggcaa tgggtctggc
    42241 aacaatggtg gcgcgtcgca aattcgacaa agcgcagcag gaaggtggca aagccgctct
    42301 ggtacttggt ctgtgcttta tttcggaagg tgcaattccg tttgctgctc gtgatccgat
    42361 gcgtgtgcta ccgtgctgta tcgtgggtgg ggcgctgact ggcgcaatct caatggcgat
    42421 tggtgcgaaa ctgatggcac cacacggtgg tctgtttgtt ctgctgatcc ctggcgctat
    42481 tacgccggta ttgggttacc tggtagcaat tattgccggt acgctggtgg cgggtttggc
    42541 ctatgccttc ctgaaacgtc cggaagtgga cgcagtagcg aaagcagcgt aataaaaggt
    42601 gtgttacagg gcagaaattt cctgccctgt aacatttgat gagcgcaaag agaatgacca
    42661 gcttgtgaat gtaattttat gaatttaaat ggttttatct ttcagtagaa acttcaaacg
    42721 ttcatcaaac actaaggtta tttcctcaag ggcagattat tttctgccct tttttagtgt
    42781 tcctgttctg tgcatcccgt cacaaattcc tcgcttttgt aattcaggca tttaatcgca
    42841 ttttgtgagc ctgctcaaat ctgcccattt tgcctctctg gcggtaattt actttacgta
    42901 aaacatgcat actatgagca catgtttaaa tatgaacaca tgtttaaacc gtctttgcag
    42961 tatctatatt cttgtggatt gacagggttg gtaatttttt accagaggac gtgctatgcg
    43021 cgaaaaggat tatgtcgtaa ttataggttc ggcgaatatt gatgtcgccg gatattcaca
    43081 tgaatcatta aattatgcgg attcaaatcc aggtaaaata aaatttacgc ctggtggagt
    43141 agggcgcaat attgcacaaa acctggcgtt gctgggtaac aaagcctggc tactgagcgc
    43201 agtaggcagt gatttttatg gtcaatcgct gctaacgcaa accaatcaat ctggcgttta
    43261 tgtcgataaa tgcctgattg tgccgggaga aaatacgtct agttatttat cattactcga
    43321 taataccggt gaaatgctgg ttgctataaa tgacatgaat attagcaacg ctattacagc
    43381 tgaatatctc gcacagcacc gtgaatttat tcagagggca aaggtcattg tcgcggactg
    43441 taatatcagt gaagaggcac tggcatggat tctggataat gccgccaacg tacccgtatt
    43501 tgtcgatccg gtttccgcat ggaaatgtgt caaagtgcgc gaccgtctaa atcagatcca
    43561 cactctgaag ccaaaccgcc ttgaagcgga aaccctgagt gggattgcgc tgtcagggcg
    43621 tgacgatgtg gcaaaagttg ctgcctggtt ccatcaacat ggcctgaacc gactggtatt
    43681 gagcatgggc ggcgacggcg tttattacag cgatatcagc ggtgaaaatg gctggtctgc
    43741 gccgatcaaa accaatgtta ttaatgttac cggagcgggc gatgccatga tggcgggact
    43801 tgcttcgtgt tgggtagacg gaatgccgtt tgccgaatct gttcgtttcg cacagggatg
    43861 ttcgtcaatg gcgctctcct gtgaatacac caataacccc gatttatcga ttgccaacgt
    43921 tatatcgtta gtggagaacg cagaatgtct aaattaaaaa tttcccctga attattacaa
    43981 atttccccgg aagtgcagga agctttaaaa aacaaaaaac cggttgtggc gctggaatcg
    44041 accattattt ctcacgggat gccgttccca caaaatgccc agaccgcaat tgaagtagaa
    44101 gaaactattc gtaaacaggg cgcagtacct gccactatcg ccattattgg cggcgtgatg
    44161 aaagtgggtt taagcaaaga agaaattgaa ttactgggtc gtgaagggca taacgtgacc
    44221 aaagttagtc gtcgcgattt accttttgtt gttgccgccg gaaaaaatgg cgcaaccact
    44281 gtggcttcaa cgatgattat tgcggcgctt gccggaatta aagtatttgc caccggggga
    44341 attggtggtg tgcatcgcgg ggcggaacat accttcgata tttctgccga tttgcaagaa
    44401 ctggcaaata ctaatgtcac cgttgtttgt gccggggcga aatctattct cgatttagga
    44461 ttaaccactg agtatttaga aaccttcggt gtgccgttaa ttggctatca gactaaagcg
    44521 ctgcctgcgt ttttctgccg taccagctcg tttgacgtca gcattcgtct cgacagcgcc
    44581 agtgaaattg cccgtgcaat ggcggtgaaa tggcaaagcg ggctgaacgg tggcctcgtg
    44641 gtagcgaacc cgatcccgga acagtttgcg atgccggagg aatctatcaa tgcagccata
    44701 gatcaagccg tcgccgaagc cgaagagcag ggcgttattg gtaaagaaag tacaccgttc
    44761 ctgctggcgc gcgttgctga actgaccggc ggtgatagcc tgaaatccaa tatccagctg
    44821 gtgttcaaca acgccattct ggcgagtgaa attgccaaag aataccagcg tctcgcgggt
    44881 taatttctga cgggcagaaa gccgtcgccc gtactcatca agcataccaa aacctggcgc
    44941 tgacgccggg tttactggca tgaagggaaa atcattatgg atataatgag aagtgttgtg
    45001 gggatggtgg tgttactggc aatagcattt ctgttgtcag tgaataaaaa gagcatcagt
    45061 ttgcgcacgg ttggagccgc actgctgctg caaatcgcta ttggtggcat catgctctac
    45121 ttcccaccgg gaaagtgggc agtagaacag gcggcattag gcgttcataa agtgatgtct
    45181 tacagtgatg ccggtagcgc cttcattttt ggttcgctgg ttgggccgaa aatggatgtc
    45241 ctgtttgacg gtgcgggttt tatcttcgcc tttcgcgtac ttccggcgat tatttttgtt
    45301 actgcgctca tcagtctgct gtactacatt ggcgtgatgg ggctgctgat tcgcatcctt
    45361 ggcagcattt tccagaaagc tcttaacatc agcaaaatcg aatcttttgt cgcagtcaca
    45421 actattttcc tcgggcaaaa tgagatcccg gcgatcgtta aaccgtttat cgatcgcatg
    45481 aatcgcaacg agttgtttac cgcaatttgt agcgggatgg cgtccattgc tggttcgatg
    45541 atgattggtt atgccggaat gggcgtacca attgactacc tgttagcggc atcgctgatg
    45601 gcgatcccag gtggtatttt gtttgcacgt attcttagcc cggccaccga gccttcacag
    45661 gtcacatttg aaaatctgtc gttcagcgaa acgccgccaa aaagctttat cgaagcggcg
    45721 gcgagcggtg cgatgaccgg gttgaaaatc gccgccggtg tggcgacggt ggtaatggcg
    45781 tttgtcgcaa ttattgcgct gatcaacggc attatcggcg gaattggcgg ctggtttggt
    45841 ttcgccaatg cctctctgga aagtattttt ggctatgtac tggcaccgct ggcgtggatt
    45901 atgggcgtgg actggagtga tgccaacctt gcgggtagcc tgattgggca gaaactggcg
    45961 attaacgaat tcgtcgctta cctgagtttc tccccatacc tgcaaacggg cggcacgctg
    46021 gaagtgaaaa ccgtggcgat tatctccttt gcgctttgtg gttttgctaa ctttggttct
    46081 atcggtgttg tcgttggcgc attttcggct atttcgccaa aacgtgctcc agaaatcgcc
    46141 cagcttggtt tacgggcgct ggcagcagca acgctttcca acctgatgag cgcgaccatt
    46201 gccgggttct ttattggttt agcttgagtt tcattgccgg atgtacgcgc ttattaagcg
    46261 ttgcatccgg caatcgcgct attgcaaata atttttactg catcatcccg gagaatttat
    46321 tctccgggtc catctccagc gccagtcctg acaactgctt tctgttttta attagatatc
    46381 ctttcttgct tttaattaat aaaccgtcgt gaatgaattg cgcgagaaca tataaaagat
    46441 gtcgataaga aacgcctaaa tactctgctg cctgggtatg tttttcgtga taaagatcac
    46501 cctcctgcga gagtaatata aatgctgcca ggcgattcac taacggaaat gattgattct
    46561 gagttaaaga aacaatatta cgataatttt tatgacttaa ggtgacgcag agttttcgta
    46621 aaaatagcgt atcgtttaat aacagtggac ggtaatgttt catagggagc gcaaggcacc
    46681 aacactcttc aatagcctgt accgcacgcg gttcatggtc tttatcgatt aactcaatct
    46741 cgccaataaa acagggggcg gcaaagaaat cgatcagcga tacgcgacca ttagctagcg
    46801 tggcgtaaag cctggcgcgg cctcgcgtca ggtaaaacag ccaggacggc tgttgccctt
    46861 cctgcacaat gtagtcacgc gctaaaaaat gaaacagccg cgtatcggct gaaacatccg
    46921 ttaaaaagca atccttaaac gcggattcac tcatcaattg ctgcttaaga tcattattgt
    46981 ggatttcttt cacgatgtat atccgctatg agatttctca tatttagcat acgtacgctg
    47041 ttgttaagat tcaatcatcg gcccggtcga attttctttg ctgtagaaaa aataacgcaa
    47101 ctggaaacag aggaaataaa caatggaaaa gagaaaaatt attctggatt gtgatccggg
    47161 tcatgatgat gctattgcta taatgatggc ggcgaaacat ccggcgattg atttattagg
    47221 tatcaccatt gttgcgggta atcaaaccct cgataaaaca ttaattaatg gcctgaatgt
    47281 ttgccagaaa ctggagatta atgttccggt ttatgcgggg atgccacagc ccattatgcg
    47341 taaacaaatc gttgccgata atattcacgg tgaaaccgga ctggatggcc cggtattcga
    47401 gccgctgacc cgccaggcag aaagtaccca cgcggtgaaa tacatcattg ataccctgat
    47461 ggcaagcgat ggcgatatca ctttggtgcc ggttggtccg ctttcaaata tcgcggtggc
    47521 aatgcgtatg caacccgcga tcctgcccaa aatccgtgaa attgtgctga tgggcggcgc
    47581 ttacggtaca ggcaacttca cgccatctgc cgagttcaac atctttgctg acccggaagc
    47641 agcccgcgtg gtgttcacct ctggcgttcc attagtgatg atgggcctcg atctcaccaa
    47701 ccagaccgtt tgcaccccgg acgtgattgc tcggatggaa agggcaggcg gccctgccgg
    47761 agagctgttc agcgacatca tgaacttcac tctcaaaacg cagttcgaaa actacggcct
    47821 ggctggcggc ccggtgcacg acgccacctg catcggttat ctgattaacc ctgatggcat
    47881 taaaacccag gagatgtacg tcgaagtgga cgtcaacagt ggcccttgct atgggcgcac
    47941 cgtctgcgac gagctgggcg ttcttggcaa gcccgccaat accaaagtcg gcatcactat
    48001 tgataccgac tggttctggg gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca
    48061 ttaagcgttg ataacaagcc gggccgatag cgcaagatgc aggcccggct atttctgatg
    48121 ggcagaaagc cgtcgcccaa tatgacatga agagataatc actatggatg tcatgagaag
    48181 tgttctggga atggtggtat tgctggcaat tgcatttttg ctgtcagtaa acaagaagaa
    48241 gatcagcctg cgtaccgttg gcgcggcgtt agtgttacag gttgtgattg gcggcattat
    48301 gctttggtta ccgccagggc gttgggtcgc tgaaaaagtc gcttttggcg tgcataaagt
    48361 gatggcgtac agcgacgcgg gtagcgcatt tatcttcggt tcgctggtcg ggccgaaaat
    48421 ggacacgctg tttgatggcg caggatttat ctttggtttc agagtattac cggcaattat
    48481 cttcgttaca gcactggtga gtattctcta ctacatcggt gtgatgggga ttttaattcg
    48541 cattctcggc ggtatcttcc agaaagcgtt aaatatcagc aagatcgagt catttgtcgc
    48601 ggtcactacc attttcctcg ggcaaaacga aattccggca atcgtcaaac cctttatcga
    48661 tcggctgaat cgcaatgaat tatttacagc gatttgtagt ggcatggcct cgattgctgg
    48721 ttcgacaatg attggttacg ccgcactggg cgtgcctgtg gaatatctgc tggcggcatc
    48781 gttaatggcg atccctggcg ggatcttgtt tgcccgcctg ttaagtccgg caacggaatc
    48841 ttctcaggtt tcctttaata acctctcttt caccgaaaca ccgccaaaaa gcattattga
    48901 agccgctgcg acaggggcaa tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt
    48961 tatggcattt gtcgccatca ttgcgttaat taacggtatt atcggcggcg ttggcggctg
    49021 gtttggtttt gaacatgcct cgctggagtc cattttaggt tacctgctgg ccccacttgc
    49081 gtgggtaatg ggtgttgact ggagtgatgc aaatcttgcc gggagtttga ttggacagaa
    49141 actggcaata aatgaatttg tcgcttatct caatttctca ccctatctgc aaacggctgg
    49201 cactctggat gctaaaaccg tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt
    49261 tggttctatc ggggtggtgg tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga
    49321 aatcgcccag cttggtttac gcgcgctggc ggcggcgaca ctttctaatc tgatgagtgc
    49381 caccattgcc gggttcttta ttggtttagc gtagtgcgca atttatgagc cggataagat
    49441 gcggtcaagc gtcgaatccg gcaacattgt tacaccattg gcactaatga aagcgcgtta
    49501 tcggcagaca gggtagggtt gtttaagctg ccgctggcgc gcgaaattgc cgcgcaggct
    49561 accgcaaaac gggcgctgtc gcggaaactg tatccttcaa gaaagctata taccaggccc
    49621 gccataaaac catcgtcagc accaaaactg tctactgtcg tatgcgctgg cgcggtcagc
    49681 aaaaattgtt ctccatcctt atcgctgcaa aatacagatt catctttcag ataaacaaaa
    49741 atgcgtttaa caccttgttg atgcagcgaa tttacagctc tgatgcgatc gttgtcgtct
    49801 ttaatcggtt gcccccacaa aatttccagt tcattttgtg ttggtttcag agtgtggatc
    49861 cggctgtacc agttttttac tttgttcgct ttaaattcgg aaacggtatc gacaagcatc
    49921 ggaatttcat cagcaatggt aaagacccac tccagggctt caggtgtgag attacaatca
    49981 gccagcacca caccagcatg gcgaatcaag tcccgtgaag tattgagcag ttgaggcgtt
    50041 agctgttgca gaatatgtgt gtcattgatt gccaggatag tttcttcctg cttattagcg
    50101 atagcgagat aagttgcagt gctgtgtcca tgcagacgaa tgcagtttga aacgttcacg
    50161 cccgcgcggc gcgtttcttc cagtaatgtc tcgccgtaaa aatcgttgcc aatagctgat
    50221 attaaatgca catcccgacc aagcagagcc aggttatgcg cgatattacg tccgacgccg
    50281 cctgcggaac agtgaacaga acctggatga gaggctgctt gcgggtaacg gatatccgcc
    50341 attccgcgaa tatccatatt gattgcaccc acaaccacac agtaatcctg ctcggtaagg
    50401 atatatcctt tgcctttaat caaacccttg cgcatcaaat ccataatatg agcggcaaca
    50461 cgtgagcagc tgatttgcag aatatcggcg atttcattct gttgaatcag cgggttacgc
    50521 cgtaaaattt taagaatctg cttttccctg tcgttcatcg atcaggccac cgctttttca
    50581 gtttgttgcg ctttcagcca ggcgatctct tctgcccaga tatccgggtt gatggtttca
    50641 aggatcagcg gaatgccgtc gaaacggtcg tcctgcatga tccagcggaa cgcatcatga
    50701 ccgatattgc cttcaccgag gctatgatgg cggtcaacgc ggctgccaaa ggtgcttttc
    50761 gcatcgttaa ggtgcatccc gcgcagatac ttaaagccaa caatacgggc aaaatccgcg
    50821 aacgttttct cgcattcggc tggagtacgc aaatcatacc cggcagcgaa agcatggcag
    50881 gtatcaatgc agacgccgac acgggattta tcttccacgc cgtcgataat cgccgcgaga
    50941 tgttcgaatt taaaccctaa gttactgccc tgaccggcgg tgttttcaat caccgctgtt
    51001 acaccttgag ttttatccag cgcaatgttg atggattcgg caatacgcgc aaggcaatcc
    51061 tcttctgaaa tctgcatcag atggctgcca gggtggaagt tgagcaaaga aagccccagt
    51121 tgttcgcaac gctgcatttc atctataaag gcatcgcgcg atttttccag agcttcagtg
    51181 accggatgtc cgaggttaat cagatagctg tcgtggggaa gaatttgcgc cgatgtgtag
    51241 tgatattttt cacaggccgc tttgaattca tcgatggttt gcgtcgtgag cggtgcggca
    51301 cgccactgac gttggttttt ggtgaacaag gcaaacgcgg ttgcgtcgat ttcggcggcg
    51361 cgaattgcgg catttgccag accgccagca gcactaacgt gcgctccaat gtatttcatg
    51421 cgaggactcc tgttaaaccc gctggaggaa aacggtaatg atagcgggtt aacaggagca
    51481 agatgtagtg gtttatgcga tgacgctttg aatcacatag ttaatcgcac caccaccaac
    51541 aatcagccag gcaaacagta ccagtgccat cagcagaggt ttcgccccgg cttttttcag
    51601 cgcgctgacg tgagtggtca gacccagcgc cgccatcgcc attgccagca ggaaggtatc
    51661 cagcgttacc agcatgttca ccacgctctg cggtaacagg tggaacgagt taaagatggc
    51721 aacgacgatg aacaatatgg caaaccacgg aatagtgatt ttgcttttct cgccgctgtt
    51781 cgccccagac agctgtttaa cacgcgccgc cagcaggatg aggaacggag ccagcatcat
    51841 cacgcgcagc attttggaaa taactgctgc gttttccgca tccgggctga tggcatgacc
    51901 tgccgccacc acctgcgcca cttcgtgcac agtagaacca atgtagatac cgaaagtttc
    51961 cggactaaac cattgagaca tcagcggata tatcgccggg tagaggaaaa tcgcgacggt
    52021 cccgaagata acaacggttg caacagccac ggttacttta ctggcttccg ctttcactac
    52081 cggctcagtc gccagtaccg cggcagcacc acagatactg ctaccggcac cgatcaacca
    52141 gctggtgtgc ttatccagac caaacacttt ctgccccagg aagcaagcca gcaggaaggt
    52201 actggacagc gtcagcacgt caatgatgat cccactgata ccgacatcgg caatttgcga
    52261 gaacgtcaga cggaagccat aaagaatgat acccagacgt aataaatatt gcttggcaaa
    52321 cagcacacca ccgtcacagc ttttccagat gtgcgggtaa atggtgttgc ctaaaaccat
    52381 ccccaacaag attgcgaggg tgagggcact aaacccggca cccgcaaccg cgggaatgga
    52441 accaccccac agggcgaccc cggtgataac tgcactcagg gctaaccccg gaataaaatg
    52501 ccacagtgta cgatgttgtt tctgtaaggt gatattcgtc ataaccctct cctttaaccg
    52561 ggcataaggt tacgactaac tggtttaaaa ataaaattga ttatatattt ataattaatc
    52621 tttataagtg gtaagcgact atgcacatca ccctccggca gttggaagtt tttgcagaag
    52681 tattgaaaag tggatcaacc acccaggcgt cggtgatgct ggcgttgtcg caatcagcag
    52741 tgagcgcagc cttgaccgac ctggaagggc agcttggcgt tcaactgttt gatcgcgtgg
    52801 ggaaaagact ggttgttaat gaacacgggc ggctgctcta tccgcgagcg ttggcactgc
    52861 ttgaacaggc ggttgaaatc gaacaactgt ttcgcgaaga caacggcgcg attcgtatct
    52921 atgccagtag taccatcggt aactacattc tgcctgcagt tatcgctcgt tatcgccatg
    52981 attatccgca gttgccgatt gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg
    53041 tgctggattt ccgcgttgat attggtttta ttgaaggacc gtgccacagc actgaaatta
    53101 tttctgaacc gtggctggaa gacgagctgg tggttttcgc cgcgccgact tcgccgttgg
    53161 cccgtggtcc ggtcacctta gagcagctgg ccgctgcgcc gtggatcctg cgtgaacgcg
    53221 gttccggcac gcgggagatt gtcgattatc tattgctgtc acatttaccg aagtttgaga
    53281 tggcgatgga attaggtaac tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa
    53341 ttagttgcct gtcgcgacgg gtgattaaag atcaattgca ggcaggcaca ttaagtgaag
    53401 ttgcggtccc tctgccgcgc ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac
    53461 acctttccaa cgcgctacgg cgctttctgg actattgcga tcccgcgaat gtaccgcgtt
    53521 aagttgctgt acaagaacat gctggtgctg tgtcgatttc gtgacgcagc gccctcagca
    53581 tgcattcgcc agaaaagaga ttggctgctt tacttataat ccctgggcga tcatgaaggt
    53641 gtcttataac cgtgtatttc tgccggaagg attgccaatc gtctgctaca atcgcgcctc
    53701 atttttaaga tggatagcat ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg
    53761 gcttacgccg tgaattaaag gcgcgtcacc tgacgatgat tgccattggc ggttccatcg
    53821 gtacaggtct ttttgttgcc tctggcgcaa cgatttctca ggcaggtccg ggcggggcat
    53881 tgctctcgta tatgctgatt ggcctgatgg tttacttcct gatgaccagt ctcggtgaac
    53941 tggctgcata tatgccggtt tccggttcgt ttgccactta cggtcagaac tatgttgaag
    54001 aaggctttgg cttcgcgctg ggctggaact actggtacaa ctgggcggtg actatcgccg
    54061 ttgacctggt tgcagctcag ctggtcatga gctggtggtt cccggataca ccgggctgga
    54121 tctggagtgc gttgttcctc ggcgttatct tcctgctgaa ctacatctca gttcgtggct
    54181 ttggtgaagc ggaatactgg ttctcactga tcaaagtcac gacagttatt gtctttatca
    54241 tcgttggcgt gctgatgatt atcggtatct tcaaaggcgc gcagcctgcg ggctggagca
    54301 actggacaat cggcgaagcg ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta
    54361 tgattgtcgg cttctctttc cagggaaccg agctgatcgg tattgctgca ggcgagtccg
    54421 aagatccggc gaaaaacatt ccacgcgcgg tacgtcaggt attctggcga atcctgttgt
    54481 tctatgtgtt cgcgatcctg attatcagcc tgattattcc gtataccgat ccgagcctgc
    54541 tgcgtaacga tgttaaagac atcagcgtta gtccgttcac cctggtgttc cagcacgcgg
    54601 gtctgctctc tgcggcggcg gtgatgaacg cagttattct gacggcggtg ctgtcagcgg
    54661 gtaactccgg tatgtatgcg tctactcgta tgctgtacac cctggcgtgt gacggtaaag
    54721 cgccgcgcat tttcgctaaa ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga
    54781 cgacggtgat tgccggtctg tgcttcctga cctccatgtt tggcaaccag acggtatacc
    54841 tgtggctgct gaacacctcc gggatgacgg gttttatcgc ctggctgggg attgccatta
    54901 gccactatcg cttccgtcgc ggttacgtat tgcagggaca cgacattaac gatctgccgt
    54961 accgttcagg tttcttccca ctggggccga tcttcgcatt cattctgtgt ctgattatca
    55021 ctttgggcca gaactacgaa gcgttcctga aagataccat tgactggggc ggcgtagcgg
    55081 caacgtatat tggtatcccg ctgttcctga ttatttggtt cggctacaag ctgattaaag
    55141 gaactcactt cgtacgctac agcgaaatga agttcccgca gaacgataag aaataagttt
    55201 cctcccttcc ttgctaagcc ctctcaaccg agagggcttt ttcaattcca tttccctgac
    55261 aaatcatgcg gatataaaat ttaacatttg gattgataat tgttatcgtt tgcattatcg
    55321 ttacgccaca atcaaaaaag gctgacaaat cagaggctgg tccggctttc tgggatgatc
    55381 acctgcatac aaataagtcc accgcgatgc tgccgtacgc aaggggacgt aaagaagatg
    55441 tgagcgatac ccattttatt ttcgtagtta cctcatggag atatggaatg tttaggttga
    55501 accctttcgt acgggtcggg ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag
    55561 cggtcgatga cgatggcgaa acgatggttg tcactgcatc ttccgttgaa caaaacctta
    55621 aagatgcacc cgccagtatc agcgtcatta cccaggaaga tctgcagcga aaaccggtac
    55681 agaatctgaa ggatgtcctc aaagaagtgc ctggcgtaca actgacgaac gaaggggata
    55741 accgtaaggg cgttagtatt cgtggtctgg acagcagcta caccctgatt cttgtcgacg
    55801 gtaaacgcgt taactcccgc aatgccgtct tccgccacaa tgatttcgat ctgaactgga
    55861 tcccggtcga ttccatcgaa cgtattgaag tggtccgtgg cccgatgtcg tcgctgtacg
    55921 gttccgatgc gctcggcggt gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt
    55981 cgggtaccgt taccgtcgat accaccattc aggaacatcg cgatcgcggt gatacctata
    56041 acggtcagtt ctttaccagt ggaccattaa ttgatggtgt gctgggaatg aaagcttacg
    56101 gcagcctggc aaaacgtgaa aaggatgacc cgcaaaactc aacgaccacc gataccggag
    56161 aaacgccgcg tattgaagga ttctccagcc gcgacggcaa tgtcgaattt gcctggacac
    56221 cgaatcaaaa tcacgatttt actgccggat acggtttcga ccgtcaggat cgtgattccg
    56281 actcgctgga caaaaaccgc ctggaacgcc agaactactc cgtcagccat aatgggcgtt
    56341 gggattacgg caccagcgaa ctgaaatact acggtgagaa agtcgagaac aaaaaccctg
    56401 gcaacagcag cccgataact tccgaaagca atacggtcga cggcaaatac acgttgccgc
    56461 tgacggcgat taatcagttt ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg
    56521 atgcggtgaa cctgaccggg ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt
    56581 ttgtggaaga tgaatggcgg atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg
    56641 acgatcacga aacctacggt gaacactgga gtccgcgtgc ctacctggtt tataacgcca
    56701 ccgacaccgt aacggtgaaa gggggctggg cgacggcatt taaagcacct tctctgttgc
    56761 aacttagccc tgactggacg agcaattcct gccgtggcgc atgtaagatt gtgggtagcc
    56821 cggatctgaa accagaaacc agcgaaagtt gggagctggg gctttactac atgggtgaag
    56881 aaggctggct ggaaggggtt gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc
    56941 gtatcagcat cagccgtacg tctgacgtca atgctgcacc gggctaccaa aactttgttg
    57001 gttttacagc gaacggtgtg ccggtattta gctactacaa cgttaacaaa gcacgtattc
    57061 agggcgtgga aaccgagctg aaaattccgt tcaacgatga atggaaactg tcactcaact
    57121 acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta tccgatctgc
    57181 cgttccatac cgctaacggt acgctggact ggaaaccgct ggcgctggaa gactggtcat
    57241 tctatgtttc tggtcactat accgggcaga aacgagccga cagcgcgacg gctaaaacac
    57301 cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa gacgtcaaac
    57361 tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac tacagctata
    57421 acgaagacgg acgtcgttac tttatggcag tggattatct cttctgatga gaagatgccc
    57481 ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca ggtgatcctc
    57541 tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac gcagcgtcat
    57601 cggccatgct ttctggcgcg cggcttctgc aagtactgca ggttgcagtt ggtcagcaag
    57661 aaactgatcg ttatcaccct gatcgataag cgttgggatc gcatcctgcg cgttactggc
    57721 atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac ctaaatagcg
    57781 gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg caaaggccga
    57841 aacgctggtg tatttgcccg ggtttttcag cgccataatc agcgcaccgt gaccacccat
    57901 tgaatgaccg ctaatagcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
    57961 taattcatca cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
    58021 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
    58081 gccgcgcggg ctggtgtctg gcataaccag tacaatcccc agttccgccg ccacgcgctg
    58141 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
    58201 cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca
    58261 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
    58321 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
    58381 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
    58441 tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
    58501 ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc ccacgcaggg
    58561 cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
    58621 tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg
    58681 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
    58741 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
    58801 tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa atcaccctca
    58861 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
    58921 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
    58981 attcggtgat cggtctgtca aaaattaacc gtattgtgca gttctttgcc cagcgtccgc
    59041 aggtgcagga acgtctgacg cagcaaattc tcattgcgct acaaacgctg ctgggaacca
    59101 ataacgtggc tgtctctatc gacgcggtgc attattgcgt gaaggcgcgt ggcatccgcg
    59161 atgcaaccag tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
    59221 cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg atttaaaggc aggaaccatg
    59281 gagcgcaacg tcacgctcga ctttgttcgc ggcgtcgcca ttctggggat cctgctatta
    59341 aacatcagcg cctttgggct accaaaggcg gcttatctta atcccgcctg gtacggcgct
    59401 attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc
    59461 ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga
    59521 cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gctttattca cggtttattg
    59581 ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg ctggcggctg
    59641 gtgcgcgatg cgccatcggt aaaaagcctt tttaataccg gcgtcatgct ttatctggtg
    59701 gggcttggcg ttttgctgtt attggggttg atttccgata gccagactag ccgcgcctgg
    59761 acgccggatg catcggctat tttatatgaa aaatactgga agcttcacgg cggcgttgaa
    59821 gcgatcagta atcgtgccga tggtgttggg aacagtttat tggcactggg cgcacagtat
    59881 ggctggcaac tggctgggat gatgctcatt ggtgccgcat tgatgcgcag tggctggctg
    59941 aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt ggcgattggg
    60001 gtgatcatta accttcctgc catcgccctg caatggcagc tggactgggc gtatcgctgg
    60061 tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat
    60121 gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt gctggcgatc
    60181 gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat ttgtaccacg
    60241 cttttttacc acctcggtct gtttatgcag tttgaccgtc tggaattgct ggcgtttgtt
    60301 attccggtat ggctggcgaa tattctcttc tctgttatct ggctgcgtta cttccgccag
    60361 gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggacc ggcaatatct
    60421 aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta accgtttcca
    60481 ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc cggcagcaag
    60541 cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg tgatgtagcg
    60601 cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag cacgctggtc
    60661 agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta tcggccaaac
    60721 gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt ggtgatggac
    60781 gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctggtcgc tcagcagcat
    60841 cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg tcacgccatt
    60901 gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc attgagtgac
    60961 gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg
    61021 ccggggtaca cccatcgttg cgtttgcctg gataatctca gcggtgcccg aatggcgacg
    61081 cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag ccacggcatt
    61141 gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca ggatattatt
    61201 ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga ggcggcgatg
    61261 gttgaactgc tggggcgcaa tctacaactt accgctgtat ttgcttataa cgacaatatg
    61321 gctgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt acatctctca
    61381 atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg
    61441 cgttatccca ttgcttcaat ggcgaaatta gccaccgaac tggccttgca gggggcagca
    61501 ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg tcgccattct
    61561 gtcgcaacac gccagaatgc ggcggcgatc actaactcaa caaatcaggc gatgtaaccg
    61621 ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg attgcaccgt
    61681 tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt aatagcgtca
    61741 taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg aattttaaaa
    61801 agtgagcttc ggcgttcagt aacacttcat taactctact gccccgccga gcatttatct
    61861 caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt gttaaccctg
    61921 tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga tactcgcatt
    61981 ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa ggctattgag
    62041 caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattcaca gaatgaccag
    62101 tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc actggcaatc
    62161 aacctagttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg gcaaaatgtg
    62221 ccggtggtat tctttaacaa agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc
    62281 tactacgttg gcactgattc caaagagtcc ggcattattc agggcgattt gattgctaaa
    62341 cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca gttcgtactg
    62401 ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt gattaaagaa
    62461 ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgctat gtgggatacc
    62521 gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa
    62581 gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact gaaagcacac
    62641 aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct ggcgctggtg
    62701 aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc gaaagcgacc
    62761 tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac caactggaaa
    62821 atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa cctggctgaa
    62881 ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat tgtatggcca
    62941 acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata cttgttggaa
    63001 atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt taatttaaaa
    63061 gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta
    63121 ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt ccagggtaaa
    63181 gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat ggtacaccag
    63241 gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctggg gcgatatccc
    63301 accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat
    63361 gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg
    63421 cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat tatggatgaa
    63481 ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat tcgtaaatta
    63541 aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat cttccagtta
    63601 tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc gctggcagga
    63661 ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca gcgttttcct
    63721 gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc
    63781 cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct cggtattgcc
    63841 gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat tcgcgagaaa
    63901 tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc caacgaagcc
    63961 ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat ttatgcctat
    64021 ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa taaagttggt
    64081 ttactggata actcgcggat gaaaagcgat acccagtggg tgattgattc gatgcgggta
    64141 aaaacgccgg gtcatcggac gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg
    64201 attattggtc gctggctact aacgcaacca gaaatattaa tgctcgatga accgacgcgc
    64261 ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa
    64321 ggcaagggga ttattattat ctcctctgaa atgcctgagt tgttagggat aacagaccgt
    64381 attctggtca tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa
    64441 aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc catgagtgcg
    64501 ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt cgttctttta
    64561 gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt gaacttaagt
    64621 aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt
    64681 gtcacccagg ggaccgatct ttctgctggt cgtcaggtag ggctggcggc agtggtggct
    64741 gcgacattat tgcagtccat ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg
    64801 ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg tttgatcaac
    64861 ggtctgatta tcgcttatct caacgtgacg ccgttcatta ccacgctcgg cacgatgatc
    64921 atcgtctatg gaatcaactc gctctattac gactttgtcg gggcgtcgcc aatttctggt
    64981 tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag tttccgtctc
    65041 tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa
    65101 acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc aaaagtatct
    65161 ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt gttctatgcc
    65221 tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct cggctttatg
    65281 tatgaactgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg
    65341 gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa ctatggcctg
    65401 acgtacatcg gcgtaaaccc atactggcag tacatcatca aaggggcgat tattatcttc
    65461 gccgtagcgc tggattcact gaagtacgcg cgtaagaagt gaaatgattg tggtccgcat
    65521 tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg ataaggcgtt
    65581 cacgccgcat ccggcgacag gagccaaatg ccggatgcaa cgctggcgcg ttttatccgg
    65641 cctacaatga gctcaacata ctgatttgta tggttttgta ggccggataa ggcgttcaag
    65701 ccgcatccgg cgacaggaac caaatgccgg atgcgtctct ggttttacaa cgttaccggg
    65761 tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc caccgggcag
    65821 acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg tgtgcgggtt
    65881 ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata acagcgtcca
    65941 cagccaacac atttatcaag attgatacgg ggatagacaa tataactgcg gtctaaatct
    66001 tccgacggga caatattgtt attcgccaga cctaccatct cctgcagcga atcaaatccc
    66061 tgatcggcga gataatgcga caacccgctt gccatatctt ccactatccg atacccgtac
    66121 tgcatgatgc cggtggtcac ctgtaacgtt gctgcgccga gcaataaaaa ctcagccgca
    66181 tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag ttctggatgg
    66241 gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc ggaatatccg
    66301 gaaatactcg attttccgtt aacgatcggc ataccgattt tctgattaag atcgatattg
    66361 gtgatggatt taacggtgtt aatcgccgca atgccatctg cgccgccgcg cttcgccgcc
    66421 agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagcat tggcagcgtg
    66481 gaaccccgtt tcactgcccg acaatatttt tctaccagct ccgggctttg cccgacatcg
    66541 ctacccatcg catgagaagt catttgcgga caggagaagt tacactcgat catatccgcg
    66601 ccagcttctt gcaccaggcg cgccagctcc tcccattgct gctcattttc ccccatgatc
    66661 gaagcgatca atactttgtc cgggtaatct tccttcagct gacgcagggc ggccagattc
    66721 tcttccaacg gatgttcagc aatctgctcc atatttttga agccgataaa accggtatct
    66781 tctttcacca gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt
    66841 ttaaacacca cgccgcccca acctgtgtcg taggctttgg cacacatctc atagcagttg
    66901 cctacaggcg aagaagagag gcagaacggg ttgggaaact tcacgccgca aaaagtaatc
    66961 gaaagatctt tcgttaacat gagcaagctc cctctaaata gtgatgaatc gcctcggcgg
    67021 cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag
    67081 cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg ttacgctgcg
    67141 gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat
    67201 ctgccgccat cgtcagttcg cccggtaacc gtacatgctt aaacgtgact ttattgcctt
    67261 ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa
    67321 actctttttc gcttgccgga aactcatcta actcttcacg cgctacgcag gttaccgcct
    67381 gacagccgag aactttcagc gtgctggcta cgtccatcgc aacatcaccg ccgccgataa
    67441 ttaatgcgct ttgtggaatg ctgatatcgc cttgcgcctg tcgtgcacgt tgcaggaagt
    67501 ccacggcaat ctcaacgtcg ctatgctcaa acagcgataa cccggaaccg ctcgataacc
    67561 ccacggtgac cagtaccgcg cggttttctg ctttcagctg ctcaagggtg agtgtgttac
    67621 cgacttcgtt gttgcactta atggtcacgc ccattttttc aatacgggcg atctctgcat
    67681 ccagcactga ttgcggtaac cggaattgcg gaataccgtt acgcaaccag ccaccggggt
    67741 gcgcttcttt ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtt acactggcct
    67801 gcaatccggc aggacctgcg ccaataatcg cgactttgcc gagcgtttta gtaccgggct
    67861 gataaatttc catcccagtt tgttgttcaa aatcagtaac aaaacgctgt aagcgaccga
    67921 tatcaatggg agtatcaaca ccggcacggg tacaaccgct ttgacataat ttctccgtcg
    67981 ggcacactct ggcacaaacg gcaccgaggg cattattttc gcgaattgtc tcggcagcgc
    68041 ctttaaaatt acgaaagtag attgagcgaa taaatttccc cggatcggtc tgtgccgggc
    68101 aagcctgact acagggagcg tcatgacata ataaacagcg agaggcttct ttaatcgcca
    68161 gtaaaggcgt aaaagccgga gtgagttcat ccagataatt ttgttgcggc atgtgatctt
    68221 ccttaatgct atgagtaatt aaggcgaacg ctaaataaat attgcgtgct gatattaagg
    68281 ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgcg gttcagaatg
    68341 ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac ag
//
LOCUS       DAZFAH010000003        65732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFAH010000003 DAZFAH010000000
VERSION     DAZFAH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.003"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..585
                     /gene="yraH"
                     /locus_tag="ACHJWB_000125"
     CDS             1..585
                     /gene="yraH"
                     /locus_tag="ACHJWB_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4311830.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            665..1360
                     /gene="yraI"
                     /locus_tag="ACHJWB_000126"
     CDS             665..1360
                     /gene="yraI"
                     /locus_tag="ACHJWB_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4311831.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKIADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1390..3906
                     /gene="yraJ"
                     /locus_tag="ACHJWB_000127"
     CDS             1390..3906
                     /gene="yraJ"
                     /locus_tag="ACHJWB_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4311832.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSNNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3917..5008
                     /gene="yraK"
                     /locus_tag="ACHJWB_000128"
     CDS             3917..5008
                     /gene="yraK"
                     /locus_tag="ACHJWB_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4311833.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(5051..5911)
                     /gene="rsmI"
                     /locus_tag="ACHJWB_000129"
     CDS             complement(5051..5911)
                     /gene="rsmI"
                     /locus_tag="ACHJWB_000129"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4311834.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            5975..8011
                     /gene="lpoA"
                     /locus_tag="ACHJWB_000130"
     CDS             5975..8011
                     /gene="lpoA"
                     /locus_tag="ACHJWB_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4311835.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVRAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7969..8364
                     /gene="yraN"
                     /locus_tag="ACHJWB_000131"
     CDS             7969..8364
                     /gene="yraN"
                     /locus_tag="ACHJWB_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4311836.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMRESRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8384..8974
                     /gene="diaA"
                     /locus_tag="ACHJWB_000132"
     CDS             8384..8974
                     /gene="diaA"
                     /locus_tag="ACHJWB_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4311837.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8984..9559
                     /gene="dolP"
                     /locus_tag="ACHJWB_000133"
     CDS             8984..9559
                     /gene="dolP"
                     /locus_tag="ACHJWB_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4311838.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9673..10713)
                     /gene="yraQ"
                     /locus_tag="ACHJWB_000134"
     CDS             complement(9673..10713)
                     /gene="yraQ"
                     /locus_tag="ACHJWB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4311839.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQVDANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10786..11421)
                     /gene="yraR"
                     /locus_tag="ACHJWB_000135"
     CDS             complement(10786..11421)
                     /gene="yraR"
                     /locus_tag="ACHJWB_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4311840.1"
                     /translation="MSQVLITGSTGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDIALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWQKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11549..12067
                     /gene="yhbO"
                     /locus_tag="ACHJWB_000136"
     CDS             11549..12067
                     /gene="yhbO"
                     /locus_tag="ACHJWB_000136"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4311841.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12047..12490)
                     /gene="yhbP"
                     /locus_tag="ACHJWB_000137"
     CDS             complement(12047..12490)
                     /gene="yhbP"
                     /locus_tag="ACHJWB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4311842.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12541..12843
                     /gene="yhbQ"
                     /locus_tag="ACHJWB_000138"
     CDS             12541..12843
                     /gene="yhbQ"
                     /locus_tag="ACHJWB_000138"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4311843.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGTGFAELLSSLQTPEIKSD"
     gene            complement(12830..13333)
                     /gene="yhbS"
                     /locus_tag="ACHJWB_000139"
     CDS             complement(12830..13333)
                     /gene="yhbS"
                     /locus_tag="ACHJWB_000139"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4311844.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13327..13851)
                     /gene="ubiT"
                     /locus_tag="ACHJWB_000140"
     CDS             complement(13327..13851)
                     /gene="ubiT"
                     /locus_tag="ACHJWB_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4311845.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14060..15055
                     /gene="ubiU"
                     /locus_tag="ACHJWB_000141"
     CDS             14060..15055
                     /gene="ubiU"
                     /locus_tag="ACHJWB_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4311846.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15064..15942
                     /gene="ubiV"
                     /locus_tag="ACHJWB_000142"
     CDS             15064..15942
                     /gene="ubiV"
                     /locus_tag="ACHJWB_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4311847.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16023..17030
                     /gene="yhbW"
                     /locus_tag="ACHJWB_000143"
     CDS             16023..17030
                     /gene="yhbW"
                     /locus_tag="ACHJWB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO4311848.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17148..18392)
                     /gene="mtr"
                     /locus_tag="ACHJWB_000144"
     CDS             complement(17148..18392)
                     /gene="mtr"
                     /locus_tag="ACHJWB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4311849.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18546..20435)
                     /gene="deaD"
                     /locus_tag="ACHJWB_000145"
     CDS             complement(18546..20435)
                     /gene="deaD"
                     /locus_tag="ACHJWB_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4311850.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20428..20508)
                     /gene="yrbN"
                     /locus_tag="ACHJWB_000146"
     CDS             complement(20428..20508)
                     /gene="yrbN"
                     /locus_tag="ACHJWB_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4311851.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20615..21499)
                     /gene="nlpI"
                     /locus_tag="ACHJWB_000147"
     CDS             complement(20615..21499)
                     /gene="nlpI"
                     /locus_tag="ACHJWB_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4311852.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21608..23743)
                     /gene="pnp"
                     /locus_tag="ACHJWB_000148"
     CDS             complement(21608..23743)
                     /gene="pnp"
                     /locus_tag="ACHJWB_000148"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4311853.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23990..24259)
                     /gene="rpsO"
                     /locus_tag="ACHJWB_000149"
     CDS             complement(23990..24259)
                     /gene="rpsO"
                     /locus_tag="ACHJWB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4311854.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24408..25352)
                     /gene="truB"
                     /locus_tag="ACHJWB_000150"
     CDS             complement(24408..25352)
                     /gene="truB"
                     /locus_tag="ACHJWB_000150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4311855.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25352..25753)
                     /gene="rbfA"
                     /locus_tag="ACHJWB_000151"
     CDS             complement(25352..25753)
                     /gene="rbfA"
                     /locus_tag="ACHJWB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4311856.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25917..28589)
                     /gene="infB"
                     /locus_tag="ACHJWB_000152"
     CDS             complement(25917..28589)
                     /gene="infB"
                     /locus_tag="ACHJWB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4311857.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKFADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28614..30101)
                     /gene="nusA"
                     /locus_tag="ACHJWB_000153"
     CDS             complement(28614..30101)
                     /gene="nusA"
                     /locus_tag="ACHJWB_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4311858.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30129..30581)
                     /gene="rimP"
                     /locus_tag="ACHJWB_000154"
     CDS             complement(30129..30581)
                     /gene="rimP"
                     /locus_tag="ACHJWB_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4311859.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30788..30864)
                     /locus_tag="ACHJWB_000155"
     tRNA            complement(30788..30864)
                     /locus_tag="ACHJWB_000155"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30828..30830),aa:Met,seq:cat)
     gene            31212..32555
                     /gene="argG"
                     /locus_tag="ACHJWB_000156"
     CDS             31212..32555
                     /gene="argG"
                     /locus_tag="ACHJWB_000156"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4311860.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKVK"
     gene            32739..33693
                     /locus_tag="ACHJWB_000157"
                     /pseudo
     CDS             32739..33693
                     /locus_tag="ACHJWB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(33768..33854)
                     /locus_tag="ACHJWB_000158"
     tRNA            complement(33768..33854)
                     /locus_tag="ACHJWB_000158"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33818..33820),aa:Leu,seq:gag)
     gene            complement(33869..34201)
                     /gene="secG"
                     /locus_tag="ACHJWB_000159"
     CDS             complement(33869..34201)
                     /gene="secG"
                     /locus_tag="ACHJWB_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4311861.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34429..35766)
                     /gene="glmM"
                     /locus_tag="ACHJWB_000160"
     CDS             complement(34429..35766)
                     /gene="glmM"
                     /locus_tag="ACHJWB_000160"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4311862.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(35759..36607)
                     /gene="folP"
                     /locus_tag="ACHJWB_000161"
     CDS             complement(35759..36607)
                     /gene="folP"
                     /locus_tag="ACHJWB_000161"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4311863.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(36697..38631)
                     /gene="ftsH"
                     /locus_tag="ACHJWB_000162"
     CDS             complement(36697..38631)
                     /gene="ftsH"
                     /locus_tag="ACHJWB_000162"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4311864.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(38731..39360)
                     /gene="rlmE"
                     /locus_tag="ACHJWB_000163"
     CDS             complement(38731..39360)
                     /gene="rlmE"
                     /locus_tag="ACHJWB_000163"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4311865.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            39486..39779
                     /gene="yhbY"
                     /locus_tag="ACHJWB_000164"
     CDS             39486..39779
                     /gene="yhbY"
                     /locus_tag="ACHJWB_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4311866.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(39935..40411)
                     /gene="greA"
                     /locus_tag="ACHJWB_000165"
     CDS             complement(39935..40411)
                     /gene="greA"
                     /locus_tag="ACHJWB_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4311867.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            40659..42092
                     /gene="dacB"
                     /locus_tag="ACHJWB_000166"
     CDS             40659..42092
                     /gene="dacB"
                     /locus_tag="ACHJWB_000166"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4311868.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42132..43304)
                     /gene="cgtA"
                     /locus_tag="ACHJWB_000167"
     CDS             complement(42132..43304)
                     /gene="cgtA"
                     /locus_tag="ACHJWB_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4311869.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43320..44285)
                     /gene="yhbE"
                     /locus_tag="ACHJWB_000168"
     CDS             complement(43320..44285)
                     /gene="yhbE"
                     /locus_tag="ACHJWB_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4311870.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44412..44669)
                     /gene="rpmA"
                     /locus_tag="ACHJWB_000169"
     CDS             complement(44412..44669)
                     /gene="rpmA"
                     /locus_tag="ACHJWB_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4311871.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(44690..45001)
                     /gene="rplU"
                     /locus_tag="ACHJWB_000170"
     CDS             complement(44690..45001)
                     /gene="rplU"
                     /locus_tag="ACHJWB_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4311872.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45260..46231
                     /gene="ispB"
                     /locus_tag="ACHJWB_000171"
     CDS             45260..46231
                     /gene="ispB"
                     /locus_tag="ACHJWB_000171"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4311873.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46459..46737
                     /gene="sfsB"
                     /locus_tag="ACHJWB_000172"
     CDS             46459..46737
                     /gene="sfsB"
                     /locus_tag="ACHJWB_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4311874.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(46785..48044)
                     /gene="murA"
                     /locus_tag="ACHJWB_000173"
     CDS             complement(46785..48044)
                     /gene="murA"
                     /locus_tag="ACHJWB_000173"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4311875.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48099..48353)
                     /gene="ibaG"
                     /locus_tag="ACHJWB_000174"
     CDS             complement(48099..48353)
                     /gene="ibaG"
                     /locus_tag="ACHJWB_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4311876.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSDDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(48513..48806)
                     /gene="mlaB"
                     /locus_tag="ACHJWB_000175"
     CDS             complement(48513..48806)
                     /gene="mlaB"
                     /locus_tag="ACHJWB_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4311877.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(48806..49441)
                     /gene="mlaC"
                     /locus_tag="ACHJWB_000176"
     CDS             complement(48806..49441)
                     /gene="mlaC"
                     /locus_tag="ACHJWB_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4311878.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(49460..50011)
                     /gene="mlaD"
                     /locus_tag="ACHJWB_000177"
     CDS             complement(49460..50011)
                     /gene="mlaD"
                     /locus_tag="ACHJWB_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4311879.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50016..50798)
                     /gene="mlaE"
                     /locus_tag="ACHJWB_000178"
     CDS             complement(50016..50798)
                     /gene="mlaE"
                     /locus_tag="ACHJWB_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4311880.1"
                     /translation="MLLNVLASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(50806..51615)
                     /gene="mlaF"
                     /locus_tag="ACHJWB_000179"
     CDS             complement(50806..51615)
                     /gene="mlaF"
                     /locus_tag="ACHJWB_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4311881.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51825..52802
                     /gene="yrbG"
                     /locus_tag="ACHJWB_000180"
     CDS             51825..52802
                     /gene="yrbG"
                     /locus_tag="ACHJWB_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4311882.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAILYWLSPILVE"
     gene            52816..53802
                     /gene="kdsD"
                     /locus_tag="ACHJWB_000181"
     CDS             52816..53802
                     /gene="kdsD"
                     /locus_tag="ACHJWB_000181"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4311883.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            53823..54389
                     /gene="kdsC"
                     /locus_tag="ACHJWB_000182"
     CDS             53823..54389
                     /gene="kdsC"
                     /locus_tag="ACHJWB_000182"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4311884.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54386..54961
                     /gene="lptC"
                     /locus_tag="ACHJWB_000183"
     CDS             54386..54961
                     /gene="lptC"
                     /locus_tag="ACHJWB_000183"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4311885.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            54930..55487
                     /gene="lptA"
                     /locus_tag="ACHJWB_000184"
     CDS             54930..55487
                     /gene="lptA"
                     /locus_tag="ACHJWB_000184"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4311886.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            55494..56219
                     /gene="lptB"
                     /locus_tag="ACHJWB_000185"
     CDS             55494..56219
                     /gene="lptB"
                     /locus_tag="ACHJWB_000185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4311887.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56267..57700
                     /gene="rpoN"
                     /locus_tag="ACHJWB_000186"
     CDS             56267..57700
                     /gene="rpoN"
                     /locus_tag="ACHJWB_000186"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4311888.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            57723..58010
                     /gene="hpf"
                     /locus_tag="ACHJWB_000187"
     CDS             57723..58010
                     /gene="hpf"
                     /locus_tag="ACHJWB_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4311889.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58128..58619
                     /gene="ptsN"
                     /locus_tag="ACHJWB_000188"
     CDS             58128..58619
                     /gene="ptsN"
                     /locus_tag="ACHJWB_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4311890.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            58665..59519
                     /gene="rapZ"
                     /locus_tag="ACHJWB_000189"
     CDS             58665..59519
                     /gene="rapZ"
                     /locus_tag="ACHJWB_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4311891.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            59516..59788
                     /gene="npr"
                     /locus_tag="ACHJWB_000190"
     CDS             59516..59788
                     /gene="npr"
                     /locus_tag="ACHJWB_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4311892.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60001..60633
                     /gene="yrbL"
                     /locus_tag="ACHJWB_000191"
     CDS             60001..60633
                     /gene="yrbL"
                     /locus_tag="ACHJWB_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4311893.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGNPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(60630..61358)
                     /gene="mtgA"
                     /locus_tag="ACHJWB_000192"
     CDS             complement(60630..61358)
                     /gene="mtgA"
                     /locus_tag="ACHJWB_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4311894.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(61355..62008)
                     /gene="elbB"
                     /locus_tag="ACHJWB_000193"
     CDS             complement(61355..62008)
                     /gene="elbB"
                     /locus_tag="ACHJWB_000193"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4311895.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62238..64574)
                     /gene="arcB"
                     /locus_tag="ACHJWB_000194"
     CDS             complement(62238..64574)
                     /gene="arcB"
                     /locus_tag="ACHJWB_000194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4311896.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGLVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(64670..65599)
                     /gene="yhcC"
                     /locus_tag="ACHJWB_000195"
     CDS             complement(64670..65599)
                     /gene="yhcC"
                     /locus_tag="ACHJWB_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4311897.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT        16237 a        17699 c        16107 g        15689 t
ORIGIN      
        1 atgaataaag ttacaaaaac agctattgtg ggcttacttg cgcttttcgc aggtaatgcc
       61 gctgcaaccg atggtgaaat agtttttgat ggcgagattt taaaatccgc ttgtgaaatc
      121 aatgactctg ataagaaaat tgaagttgct cttggtcact ataatgccga acagtttcgt
      181 tctgttggcg atcgtagccc aaaaattcca ttcactattc ctttagttaa ctgcccagtg
      241 actgggtggg agcacgataa cggcaacgtt gaagcgtctt tccgtttatg gctggaaacg
      301 cgggataatg gcaccgtccc taacttccct aatctggcaa aagtgggctc ttttgctggc
      361 acagcggcaa caggtgtggg tatccgtatt gacgatgcgg aaagcggaaa ccttatgcca
      421 ctgaatgcta tggggaatga taacacggtt tatcagatcc cagcggactc caatggtatt
      481 gtcaatgttg acctcatcgc ttactacgta tcaaccgttg aagcatcaga aatcacccca
      541 ggggaagcag acgctgtcgt taacgtaacg ctggattacc gttaatacgt tacggcgtta
      601 tctgaccttt cagataacgc ccttttcctt cctctttctc gttgtatcag gttgaaaaat
      661 aactatgtca aaacgaacat tcgcggtgat aataaccttg ttgtgtagct tctgtatcgg
      721 ccaggcgctt gcagggggta tcgttttaca gcgaacgcga gtgatctatg atgccagccg
      781 caaagaggct gcgttacctg tcgcaaacaa aggcgcagaa acgccttatt tactgcaatc
      841 atgggtagat aatatagatg gtacaagccg tgccccattt attataaccc caccgctatt
      901 tcgtcttgaa gctggcgatg actcatcact gcgaattatt aaaatagctg ataacctgcc
      961 tgaaaataaa gagtcgctgt tctacattaa tgttcgtgcc attccagcaa agaaaaaatc
     1021 agatgatgtt aatgctaacg agttgacgct ggtatttaaa acacggatca aaatgtttta
     1081 tcgccccgca cacctgaagg gacgggtaaa cgatgcgtgg acatcactgg aatttaaacg
     1141 tagtgaccat tcactcaata tatataaccc aactgaatat tacgtcgtat ttgccggact
     1201 ggcagtcgat aaaaccgatc tcacaagcaa aattgaatat atcgcgcccg gagaacataa
     1261 acagttacca cttcctgcat ctggcggaaa gaacgtgaaa tgggctgcaa tcaatgatta
     1321 tggcggcagt tccgggacag aaactcgtcc actgcaataa caaatataaa aaacacaggt
     1381 catcagggaa tgccacaacg acaccaccag ggacataaac gcacaccgaa acagttggcg
     1441 ctcatcatca aacgctgttt gccgatggtg ctcactggca gcggcatgct ttgcactacc
     1501 gctaacgccg aagagtatta tttcgacccc attatgctgg aaactacaaa aagtggtatg
     1561 caaacaactg atctgtcacg tttttcaaaa aaatacgcac aactaccagg aacttatcag
     1621 gttgatatct ggctgaataa aaagaaggtt tcacagaaaa aaattacatt taccgccaat
     1681 gcagagcaac ttctgcagcc acagtttacg gtagaacaac tacgtgagct gggtattaag
     1741 gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata gcgtgatcaa ctcgcttgaa
     1801 caaatcattc ccggtacagc tgctgaattt gatttcaatc atcagcgact taatttgagc
     1861 attccccaaa ttgcactgta ccgtgatgca agaggttacg tctccccttc tcgttgggac
     1921 gatggtatac caacgctgtt taccaactac tcgtttacag gttctaataa ccgttaccgc
     1981 cagggcaatc gtagccaacg acagtacctg aatatgcaaa atggtgctaa ttttggcccc
     2041 tggcgattac gtaactattc tacgtggaca cgcaacgatc aggcgtcaag ctggaacact
     2101 atcagtagtt atttacaacg tgatatcaag gcgttgaagt ctcagttgct tctgggagaa
     2161 agcgccacca gcggcagtat tttttccagc tacaccttta ctggcgtgca actcgcttcc
     2221 gacgataata tgttgccaaa cagccagcgc ggatttgccc caacggtacg cggtatcgca
     2281 aacagtagtg caatcgtgac tatcaggcaa aatggttatg tgatctatca aagcaacgtg
     2341 ccagcaggtg cctttgaaat taacgatctc tacccctctt ccaacagcgg cgatttagaa
     2401 gtcacgattg aagaaagtga cggtacacaa cgtcgcttta tccagcctta ttcttcatta
     2461 cccatgatgc agcgacctgg gcatctaaaa tatagcgcga ccgctggacg ctatcgcgct
     2521 gatgcaaaca gtgatagcaa ggaacccgaa tttgctgaag ccacggcaat atatggtttg
     2581 aataatactt ttacgctgta tggcggcctg ctcggttctg aagattatta tgcgctgggg
     2641 atcggtatcg gcggcacact tggcgcactg ggcgcgttgt cgatggatat caacagagct
     2701 gacacccaat tcgataacca gcactctttt catggctatc aatggcgtac gcagtacatc
     2761 aaagatatcc cggaaaccaa caccaatatc gctgttagct actatcgcta taccaacgat
     2821 ggctatttta gttttgatga agccaatacc cgcaattggg actataacag tcgccaaaaa
     2881 agtgaaattc aattcaacat cagccagaca atatttgatg gggtaagtct gtatgcctcc
     2941 ggttcgcagc aagactattg gggcaataac gataaaaaca ggaatatctc agttggggtt
     3001 tccggccagc aatggggaat tggttacagc ctgaattatc aatacagtcg ctacacggat
     3061 caaaataatg accgcgcact ctctttgaat ctcagtattc cgttagaacg ctggttaccg
     3121 cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca acatgaaatg
     3181 cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga acaaagtctg
     3241 gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc ttatggcacc
     3301 ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg cgttaccggc
     3361 ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa cgcttttgcg
     3421 cttatcgatg ctaatggggc ttctggcgtg aggatacaaa actatccggg gattgctact
     3481 gatccctttg gctatgcagt ggttccttat ctcacgactt accaggaaaa ccgtctctcg
     3541 gtagatacta cgcagctgcc cgataacgtc gatcttgagc aaacaacaca gtttgtggtg
     3601 cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta tcgcgtactt
     3661 gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc cagcaacgat
     3721 gagacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc tgggatatcg
     3781 agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca atgtcagttt
     3841 gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac ggcgcagtgc
     3901 cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt tgatatccac
     3961 atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg gtgcgacaaa
     4021 ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga caggacggga
     4081 gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct gctctgcggg
     4141 taaggcagtt aaacttgtct atatggtcag ccccgtgctt accaccactg gacatcagac
     4201 aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg caaacgacat
     4261 tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac agataaaaag
     4321 cagcaccgta tattctgctg caacccaaac gggtgtttgc cagggtgaca cgtctcgtta
     4381 tggacccgtt aatattggtg caaataccac ctttaccctg tatgtcacca agccatttct
     4441 cggctcgatg accattccga aaacggatat tgccgtcatt aaaggcgcgt gggtcgatgg
     4501 aatgggaagc ccgtctacag gtgacttcca tgatttagtc aagttatcga ttcagggaaa
     4561 tctcaccgcc ccacagtcgt gcaaaattaa tcagggcgat gttattaagg ttaattttgg
     4621 attcatcaat ggtcagaagt ttaccacccg caatgccatg ccagacggtt ttactccagt
     4681 agactttgat atcacttatg actgtggtga tacttcaaag attaaaaact cgttgcaaat
     4741 gcgcatcgac ggtacaactg gggtagtaga ccagtacaac ctggtcgcca ggcgaagaag
     4801 ttcagacaat gtgcccgatg tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa
     4861 tattcctttt cagaacggta tccttcccgt tgatccttcc gggcatggca ccgtcaatat
     4921 gcgcgcctgg ccagttaatc tggtcggtgg tgagctggaa acaggaaaat ttcagggcac
     4981 agccaccatt accgtcatgg tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc
     5041 ttcggcactg ttacccctgc tgctccagcg catacttata cagcgcattt ttcttcacgc
     5101 catgaatttc tgcgaccagc gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg
     5161 ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatga ccttcgacaa
     5221 tcagcaccat ttcgcctttg cgacggtttt catcttcctt tacccacgcc agcagttcgc
     5281 caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt
     5341 agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga cggtgggtgg
     5401 attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca tcacggcggc
     5461 cttttgattt ggcaggtaaa aagccttcgt aacagaaacg gtcagagggt aaacccgcgg
     5521 cgcttaacgc agtgatagca gcacacggcc cgggtagcgg caccacacga atccccgctt
     5581 cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt ccggcatcgg
     5641 aaaccagcgc aatgttttgc ccctcttgca gcttcgccag cagcgtttcg gctttttgtt
     5701 gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt tgcagcaata
     5761 aaccggtgtg acgagtatcc tcggcggcaa tcagatcaac ggcctgtaat acctctaacg
     5821 ctcgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa agctggccct
     5881 gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt
     5941 gagcattgcg taaaaaaata tcactggata cattatggta ccctcaacat tttctcgttt
     6001 gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg gttgtggcac
     6061 ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg attctgcctt
     6121 ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc aattactcgc
     6181 cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt ttaaccaact
     6241 accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg tagagattaa
     6301 actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca cacctgccga
     6361 tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca gccaggggcg
     6421 tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg gcgcgaaaga
     6481 aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc aggaacaggc
     6541 gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg atctgcagcg
     6601 cgtctggttt gataaccgta acgatcccga tatgatgaaa gccgggatcg ccgactggca
     6661 gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg ttaacgtaaa
     6721 agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga atggccaggc
     6781 agcggtattt ggtcgcacta ttcagcaagg ctttgaagcg gcgaaaaata tcggcactca
     6841 gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac aacctcagcc
     6901 gcaaactgcg gatggcgttg ccagcccggc acaagcctcg gttagcgatc tgaccggtga
     6961 tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg cagcggtaag
     7021 ggcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac aaccacttag
     7081 ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc cgttgctgaa
     7141 aaataacgtt gaagagttgc ttaagagcaa caccccgctg aacgtgctgg cactgaacca
     7201 gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac cggaagacga
     7261 agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc tggtgctgat
     7321 cccacgcagt tcattgggcg atcgcgtagc caatgcgttt gcgcaagagt ggcagaaact
     7381 gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac gcgcgggtgt
     7441 taacggcggt tctggtatcg ctttaacggg taccccgatt actcccagag cgacaaccga
     7501 ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg accagttcac
     7561 caataatggc ggtcgtgtcg atgcggtgta cattgtggca acgccgggtg aaatcgcttt
     7621 tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc tgtacgccag
     7681 ctcccgcagt gcgcaaggga ccgctggccc ggatttccga ctggagatgg aaggcttgca
     7741 gtacagcgaa atcccgatgc tggcaggcgg taatctaccg ttaatgcagc aggcactcag
     7801 cgcggtgaat aacgattatt cactggctcg catgtatgcg atgggcgtcg atgcctggtc
     7861 gctggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca acggtaatac
     7921 cggaagcctg acggctaacc cggattgcgt gattaacagg aagttatcat ggctacagta
     7981 ccaacaaggt caggtagtcc ccgccagtta accactaaac agaccggcga tgtgtgggaa
     8041 gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc taacgtgaac
     8101 gagcgtggcg gcgagatcga tctgataatg cgtgaaagcc ggaccaccgt ttttgtcgag
     8161 gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa
     8221 caacacaaac tattacagac tgcccgcttg tggctcgcgc gtcataatgg gagttttgat
     8281 actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga gtggattaag
     8341 gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag aaagaattaa
     8401 agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc ttccggatgc
     8461 catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca aaatcctctg
     8521 ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca tgatcaaccg
     8581 tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata atgttgtctt
     8641 aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct
     8701 gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc gcgatattgt
     8761 taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga ccggctatga
     8821 cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca ttccttcgca
     8881 tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt gcgatctgat
     8941 cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg cattatcgcc
     9001 aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg
     9061 taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca cccaggtgga
     9121 cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac agattaagaa
     9181 agaagcgcgc attaatgtaa cggcctatca gggcaaagtg ctgctggttg ggcagtcacc
     9241 aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg gtgccaacga
     9301 agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat ctaacgatac
     9361 gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga aatcgtccaa
     9421 cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga ctgaacgtga
     9481 agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg taaccacagc
     9541 gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg ccgcgtctta
     9601 tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc atccgacatg
     9661 ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag acactgccac
     9721 catcgcccct gtcagccata aggctttcgc cgggaatgct ttgcgcagca taatcagtga
     9781 cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg ttcccatacc
     9841 tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga caaacaagca
     9901 tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac catcggcatg
     9961 ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt aaaccgggat
    10021 cgtactccag aaaagcgtcc atagcgccct gccccagcgg ctaaaaaaac cgctctgtgc
    10081 ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca cccatttttg
    10141 caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa tcgccgcaaa
    10201 accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca ccggattgcc
    10261 catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc ccgccgcgac
    10321 cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt
    10381 gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga tcagcacctg
    10441 aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga ggaaatagat
    10501 catcgcgtaa tccaacgccg cttgccacgg attagcatcc acctgcgcaa ggatagattt
    10561 accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt gccatttcac
    10621 ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc actgaatggg
    10681 cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata agttatgata
    10741 attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt cagctctttt
    10801 gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca tcgcctctgc
    10861 cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg gtaacaaacg
    10921 gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat
    10981 cgacgggcga gcaatggtca atttctgcca gttctgggca attaatgctt cttccatctc
    11041 ccctttgacg cggttataga aaaacggcga gtgggcattg gcccccatag cactgaccac
    11101 cagcatatgc tgcgcgccca gccgccgccc ggttaatgcg atatccacta ccagcgtgta
    11161 atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc cgagacaaca
    11221 aaacacaatg tcgatgggat cggtgacctg cgccagcgcg tcggtcagtt gcggatcatg
    11281 gggattgaac actcctggca tatcgcccaa cggacgtcgc gtcggcgcgg ctatggcgtt
    11341 aactttcggt tcgttaatca acatccgcag caggtgaccg cccaccagcc ccgttgagcc
    11401 tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt gcgctccccg
    11461 gttcatgcac caggcttaat agaccatgag gtaattatcc ccgattgtgg gaaattcgcc
    11521 tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt taatcactga
    11581 tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag ccggacacga
    11641 agtaattacc attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag
    11701 cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg ctctgctgct
    11761 accgggcggc cattcaccgg attatctgcg tggtgacaac cgttttgtca ccttcacccg
    11821 cgattttgtg aatagcggca aacctgtttt cgccatctgt cacggcccgc agttgctgat
    11881 cagtgccgat gtgattcggg ggcgcaaact gactgcagtt aaaccgatca ttattgatgt
    11941 taaaaacgcg ggcgcggaat tttacgatca ggaagtggtg gtcgataaag atcagctggt
    12001 taccagccgg acgccggacg atctgccagc gtttaaccgc gaggcgttac gcctgctcgg
    12061 tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg tcggtgaatt
    12121 tgatttcatc gagccggatt tcccataccg gggccgacag cattctggca accggaaagc
    12181 gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg cggatctcac
    12241 ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt accgttccgg
    12301 caactgccgc ctgcggcccg ctcatctgcg cgtggcgcgt tttttcttcc gtcaaaatgt
    12361 agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag agttcccctt
    12421 cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg atggcggtga
    12481 gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc gatgcagacg
    12541 atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta taccgggatc
    12601 accacggatg tcgaacgccg ctatcagcaa catcaaagcg gcaaaggcgc aaaagcacta
    12661 cgtgggaaag gtgaactgac gctggcgttt tccgcgccag tgggcgatcg ttcgctggcg
    12721 ttacgggcag aatatcgcgt taagcaactg accaaacggc aaaaagagcg tctggtagcg
    12781 gaaggcacag ggtttgcaga actgttgagc agtctgcaaa ccccggagat taaaagcgat
    12841 tgaaatgctc gtgatactca accagaccag ttacgccatt cagcgcgtca tccgctaaac
    12901 gatgtacctg gaaggcgctt tcagtgcccg gccagcggca acgcagatcg tgatgcgccg
    12961 ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat
    13021 agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgagcc agcccctgtc
    13081 cacggtattt ttcatctacc gccagcggtg ccatgccgac ccattgcagg tcttcgccct
    13141 gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca tctgtcgcca
    13201 ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc gcttccgcat
    13261 cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg ggaatttcta
    13321 ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc
    13381 cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg gcatttgctc
    13441 cagttcaatg gcgtccatca ggtttttcac atacagcccc agctccgtat cgccttcaat
    13501 caccagccga cgctggaaga agagcgtatc tggatcttgt ttacgcgccg caatcatcag
    13561 cagatcgctg gcgtcggcac taaaactcac atcagcttgc gcgttctggc tgaccaccag
    13621 tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat gaatacttaa
    13681 ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga attgccagct
    13741 taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta ccggtacact
    13801 caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca cgagttttac
    13861 tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag cggtataaat
    13921 caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc ttaaatcaaa
    13981 aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg ttttgatagc
    14041 gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa
    14101 ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg ataccaatgc
    14161 ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg tgagttttgt
    14221 ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc cggacggtta
    14281 cgcccgttgg cagcgcgccg tggatatggc ggcgcagctg ggtgccgacg cgctgatcct
    14341 cgccgacctc gccatgctgg aatacgccgc cgagcgttat ccgcatattg agcgccacgt
    14401 atcggtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc gccattttga
    14461 cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac agctggcacg
    14521 ggtcacacct gtaccactgg aagtgtttgc tttcggcagc ctgtgcatta tgtcggaagg
    14581 ccgttgctat ctgtcgtcgt atctgacggg tgagtcgcct aacaccgtgg gcgcgtgttc
    14641 tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc gcctgaacga
    14701 agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc tgtgtaaagg
    14761 gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca gtctcaatac
    14821 cctggaactg ctgccggagt taatggcggc gaatattgct tcggtgaaaa ttgaaggccg
    14881 ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg ctatcgaccg
    14941 ttgtaaggcc gatccgcaaa acttcatacc gcaaagcgcg tggatggaga cgctcgggtc
    15001 gatgtccgaa ggcacgcaga ccacccttgg cgcgtatcac cgtaaatggc agtgagaaga
    15061 gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac gctggaagat
    15121 ttttatcagc aggccgccac cagcagcgcc gacgtgattt atcttggtga agcggtatgc
    15181 agcaagcgcc gggcaaccaa agttggcgac tggctggaga tggcaaaatc gctcgccgga
    15241 agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc
    15301 gaactgaaac gctatgttga gaacggtgag tttctgattg aagccagcga tctcggcgtg
    15361 gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct gaactgctac
    15421 aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg
    15481 gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct gggcattcgc
    15541 aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta ctccgcccgc
    15601 tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ctgcattaag
    15661 tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc
    15721 attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc catgcagggg
    15781 ctggttgatg tggtccgcct gtcaccgcag ggtactgaca ctttcgcgat gctcgacgcc
    15841 ttccgcgcta atgaaaatgg cgctgcgcca ctaccgttga cggcaaacag cgactgtaac
    15901 ggctactggc ggcggctggc cgggctggag ctgcaagcgt aagtatatag ctcactttgt
    15961 taacaacttt aactactctt taatgcagta ttaaagacta atcggtaaca aagtgagctg
    16021 ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccatc cccgaaggtt
    16081 cttcagcgcg ggaagcgttc tcccactctc tcgacctcgc ccgtctggct gaaaagcgcg
    16141 gctatcatcg ctactggctg gcggaacacc acaatatgac cggcattgcc agtgctgcaa
    16201 catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg gggtctggcg
    16261 gcgtgatgtt gcctaaccac tcaccgttgg tcattgccga acagttcggc acgctcaata
    16321 cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagc gatcagcgaa
    16381 ccatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc cgcgatgtcg
    16441 cggagctggt ggactggttt gacgcccgcg atcccaatcc gaatgtgcgc ccggtaccag
    16501 gctatggcga aaaaatcccc gtgtggttgt taggctccag cctttacagc gcgcaactgg
    16561 cggcgcagct tggtctgccg tttgcgtttg cctcacactt cgcgccggat atgttgttcc
    16621 aggcgctgca tctttatcgc agcaacttca aaccgtcggc tcgactggaa aaaccatacg
    16681 cgatggtgtg catcaatatt atcgccgccg atagcaaccg cgacgctgag tttctgttta
    16741 cctcaatgca gcaagccttt gtgaagttgc gccgtggcga aaccgggcaa ctgccgccac
    16801 cgattcaaaa tatggatcag ttctggtcgc cgtccgaaca gtatggtgtg cagcaggcgc
    16861 tgagtatgtc gttggtaggt gataaagcga aagtgcgtca tggcttgcag tcgatcctgc
    16921 gcgaaaccga cgccgatgag attatggtca acgggcagat tttcgaccac caggcgcggc
    16981 tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag tgtgtcttaa
    17041 cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt agacctgata agcgaagcgc
    17101 atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta ctgatacacc
    17161 ggcagtaaat taaagctcga taaaatatgg accagtgcgt tgccaacgcc aaacaccaga
    17221 atcagcgtaa tcatcggctt gccgccccag acgcggaatt tcgggctgcc aaagcgttta
    17281 cgcgatgcgc gggctaacag cgccggaaca attgccgccc agatggtagc tgctaaacca
    17341 gcataaccaa tggcgtacag gaatccgttc gggaacagta gaccacccac aacaggcggg
    17401 gcaaaggtca gcaatgccgt tttcaagcgc cccagggctg agtcatcgaa accaaacaga
    17461 tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc taccgcaaag
    17521 tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc gcttaatgcc
    17581 tgtaccagca catcaatatt accgcccttc tctgcaatac cgataaactc cggacgcggg
    17641 atgttaccca tcgtcgccag caaccagatg gtatacagcg ccagtgccat cagcgtaccg
    17701 tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat caggcttggc
    17761 acgttaccgt gataaccaaa cgacgccagg cagaacggca atgtcatcag cagatacggc
    17821 gcataagatg cattgctttc tgcaacgttg aacaatgtcg caggctgcac atgccccaac
    17881 aggctgccaa aggtgaggaa gaaggtaatg actttcgccc ccagcacaat cgccgtcatg
    17941 cggctgacgg ccttagtact caaccacacc acaaacgcta ccagcagtgc aaaaccaaaa
    18001 cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg cagaattgaa
    18061 ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat ggaaataccg
    18121 ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa actcgaaccg
    18181 attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat acagaaccag
    18241 gtaaagatca gcgccgccat tgaccagaaa aaccacgccc cggacatgac cactggcaga
    18301 gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc aagcagcgac
    18361 ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg aaaaatagtg
    18421 cgactgcgtt gttatgcatt gcactgtacc agtacacgag tacaaaagac agaaaaaaag
    18481 ccccgatggt aaaaatcggg gctgtatata ttattttaca gattgtgttc gctgttcagc
    18541 gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg acgcggagcg
    18601 cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacggccacc ttcacggcgt
    18661 tcaccgctga agttacgacc gccttcacga cgttcgccac cgaaaccacg accaccgcca
    18721 cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt catcggcttg
    18781 ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc tttcggcagt
    18841 tcgatggtgg agtgagaagc aaacagcttg atgttaccaa tgtaacggct gctgatgtcg
    18901 ccttcgttag caatcgcacc aacgatatga cgaacttcaa caccatcatc gcggcccact
    18961 tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg acgatcttca
    19021 cggtcaccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc acggtcacgg
    19081 aattcacgtt tcggacgcat cggcgcatct ggcggtacga tcagagtacg ttcaccctgt
    19141 gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc ttctgcagtc
    19201 ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc cagctgctgc
    19261 tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc gttcggcagt
    19321 tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag acgacgctcg
    19381 cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt acgaccgata
    19441 cggtgaacgt aagactcaga atccatcggg atatcgtagt taactaccag gctgatacgc
    19501 tcaacgtcca ggccacgggc tgcaacgtcg gtcgcaatca ggatgtccag acgaccatct
    19561 ttcaggcgtt ccagtgtctg ttcacgcagc gcctggttca tgtcaccgtt cagcgcggcg
    19621 ctgttgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt tttggtacga
    19681 acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag tgcttcgttt
    19741 ttgcgcatac cccagacagt ccagtagctc tggctgatgt caggacgggt agtcacgctg
    19801 gactgaatgc gcacttcctg cggctctttc ataaagcggc gggtaatgcg acgaatcgct
    19861 tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg cgccataatg
    19921 gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc cagaaccaga
    19981 ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag acggcccgga
    20041 gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc ataacgctgg
    20101 ccgccgtata gagcaaccac atttacgccg cgcatgtgtt tagagaaatc cgtcattgct
    20161 tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg tggtgctttc
    20221 agctcaggat caagattctg caacagaggt aaagagaatg ctgcagtttt tccgctcccc
    20281 gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca ctctgcctga
    20341 attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg agccttcagg
    20401 cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct caaaattaat
    20461 ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg aaaagtgtga
    20521 accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa ggtgatggca
    20581 atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat tctgccaggt
    20641 catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc tcaacaaagt
    20701 tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc aaatccccca
    20761 gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca gcgagcgagg
    20821 tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta atgttgccca
    20881 ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg aagtgctgtt
    20941 tcaacacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa agccacagac
    21001 tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct tgcgctaact
    21061 tgtcacgacc gccgtaatat aatgcgatcc cgcgattcaa gtgcgcgtag ttgtaagttg
    21121 gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg cctgcctgcg
    21181 ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt gccagcgctt
    21241 gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac actccgcgct
    21301 catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg atttgttcca
    21361 tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg aggacttcac
    21421 ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca acgaaacacc
    21481 agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa cgtcctgttc
    21541 ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg gagggcaaat
    21601 ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca ggttgagact
    21661 gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact tccagaactt
    21721 tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg cgtttgtcag
    21781 cgatttgaga gatgtggacc agaccttctt taccgccgcc gatggcaaca aatgcgccaa
    21841 agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt tctgcagtga
    21901 tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct gcgatcttca
    21961 cagtaccgtc atcttcgatt tcgatggtag tgccagtttc ttcggtcagg gcacggataa
    22021 cagagccgcc tttaccgata acgtctttga tcttatccgg gttgatcttg atggtatgga
    22081 tacgcggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt tccattacgc
    22141 ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg atctctttgg
    22201 tgataccttc aattttgata tccatctgca gtgcagagat accgtcgcgg gaacctgcaa
    22261 ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac agtacaacgt
    22321 agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct ttgattggca
    22381 cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa gaagaaccgt
    22441 tggattcggt gatttcagac actacacgta cggtgtacgg gaatttgtcc atatccggca
    22501 tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga cgcttcggag
    22561 aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg aacaggaagg
    22621 tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagcg gtacccagcg
    22681 ttgcagtaac cagtgcctgc gtttcaccgc gggtgaacag cgcagaaccg tgagtacgcg
    22741 gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga ccgtcgatac
    22801 gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatagcg tgcagaattt
    22861 cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg atggtttcag
    22921 atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg taagcatcgc
    22981 tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt accggctccg
    23041 gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg ttctgaataa
    23101 caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct tcgctcagca
    23161 gttcagcttc agattcaacc atcagaacag cggcttcagt accggcaaca accagatcca
    23221 gtttgctctc tttcagctcg tcctgagtcg ggttcagtac gtactggtca ttgatgtaac
    23281 ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac agtgctgcgg
    23341 aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa accacggtgg
    23401 cgataacctg aacttcgttg acgaagcctt ccgggaacag cgggcgaatc gggcggtcaa
    23461 tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg aagaagctac
    23521 ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt gggaagaagt
    23581 cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg gtgtcatcca
    23641 tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc agagtcacgg
    23701 tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata tcctttctct
    23761 ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc atcctcgcga
    23821 ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc gcgcgaacct
    23881 ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt gctgccgtca
    23941 gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat tagcgacgca
    24001 gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc aggtagtcga
    24061 gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg tgatcttttt
    24121 tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt gctacctgaa
    24181 cttcggtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt ttagctgttg
    24241 cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg acgccgatct
    24301 ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg cgacgttaag
    24361 cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta cgccgggtat
    24421 tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc cataccgata
    24481 aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc accggatgta
    24541 cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt caccaccggg
    24601 tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc cgcagccgga
    24661 atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt caccatccgt
    24721 tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc gccacaaccg
    24781 agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga gcagtgaatt
    24841 tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac ggtaatcggg
    24901 cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt tttaccctga
    24961 tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa agtatccagc
    25021 gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat ttgtccgtcg
    25081 gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg tttgtcggag
    25141 tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa catgccagtt
    25201 gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat ccgtttcact
    25261 ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa aacgccgtta
    25321 atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg tccgggttaa
    25381 cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg cgcatccctt
    25441 caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg cgcatcgctt
    25501 tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg cccgctttaa
    25561 ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac gccaggtcgc
    25621 gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct ttaatttcac
    25681 gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc gggcgaccaa
    25741 attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc tttttaaaat
    25801 taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc gtcacatcag
    25861 gcaacggctg ccggatgcgg cgtaaaagcc ttatccggcc tacaacccag acaaccttaa
    25921 gcgatggtac gttggatttc gatgatctcg aatacttcga tcacatcgcc agtgcggaca
    25981 tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc gttaacgtca
    26041 tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt gtcgcgcaga
    26101 acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacaacc tgcaatggca
    26161 ccaaatttcg gtgatttgaa tacgtcacga acttccgcca gaccgataat ctgttgtttc
    26221 agttccggag acagcatacc gctcattgcc gctttcactt cgtcgatcag gttatagatg
    26281 acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc agaggcatca
    26341 gcacgtacat taaagccaac caggatggcg ttggacgccg cagccagggt ggcgtcggtt
    26401 tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc agtagacagt
    26461 ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt caggacaatg
    26521 ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag tttagatttc
    26581 tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc aacttcacgc
    26641 gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac gccggacagg
    26701 ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc cagttcgtta
    26761 cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc cttgtgcaga
    26821 gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag gaaggattcg
    26881 ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac ttccgcctgc
    26941 agcaggatag cgtccagcag ttcatcgatg ccggtacccg ctttcgcaga tacgtgtacg
    27001 aactggcttt caccgcccca ctcttccggc aggatgccat actgggagag ttcgttctta
    27061 acacgatccg gatcggcttc tggtttatca atcttgttca ctgcaaccac caccggtacc
    27121 tgcgccgctt tcgcgtgctg gattgcttcg atggtctgcg gcatcacgcc gtcgtcggca
    27181 gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gtgcacgcat tgaagtaaac
    27241 gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc aacgtggtat
    27301 gcaccaatgt gctgggtaat gccgcccgct tcgccagagg caactttcgt tgaacgaatg
    27361 tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac aaccggcgcg
    27421 cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc cagctcgttt
    27481 tcacgacgca ggataacttt gtggcccatc tcttcagcaa ccagctgtgc ggtttcctga
    27541 tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt gatgacctga
    27601 gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatggtttc gccgatcaca
    27661 acgtcacggt taacggcctg agctggcttc tggaagcctt gctgcaggga agaaccttta
    27721 cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc agcttttgat
    27781 tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc acggccacgg
    27841 ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg agaagtagtg
    27901 acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt gttttcttcc
    27961 gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt acgacgcgct
    28021 tcttcttcgg ctttacgctt gagctctgca gcttcctgct cacggcgggc tttttcagcc
    28081 tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc cgcagcttca
    28141 cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg ttcagctttt
    28201 tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc ttccgcttca
    28261 cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt gcggatcgcg tttcacaaag
    28321 gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt accaggaatg
    28381 ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga atttttctga
    28441 ttcaggtggt caatcaaagt ctgtttctct tgtgcggaca cagagtcgtc agcaaacttc
    28501 cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg tcgctctgcg
    28561 gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt ttattacgct
    28621 tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc tttttcgtcg
    28681 gtcaaccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc ttccagcgta
    28741 caaacgccac gggcggccag tttgaatgcc aaatcacgat ctatcccttc aaggttcagc
    28801 agatcgtcag ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc aatggtggcc
    28861 agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag gccttcgatt
    28921 tccaacagct ctttcatcgg cacataggcc agttcttcca gcgtcgagaa gccttcttct
    28981 accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt tggtgaaggt gtcgatcgct
    29041 gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac gttgagttcc
    29101 caaccgctca gctgcgaagc cagacgcacg ttctgaccgt tacggccaat cgcctgcgcc
    29161 aggttaccgg cttcaacggc gatatccatg gtgtgtttat cttcatccac cacgatagaa
    29221 gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg ccgggttatc atcccacagg
    29281 acgatatcga tacgctcgcc gcccagttca gtagaaaccg cctgaacacg cgcgccacgc
    29341 atacctacac aggcacctac cggatcgata cgtttatcgt tggttttcac cgcgattttc
    29401 gcacgagaac ccggatcgcg agccgctgct ttaatttcaa tcacttcttc gccgatttct
    29461 ggcacttcaa tacggaacag ttcgatcagc atttccggct tggaacgagt gacgaacagt
    29521 tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc cacgaacgcg gtcgccaggg
    29581 cggaagtttt cacgcggcag catatcttcg cgcaggatca cggcttcagc gttgttgccc
    29641 agatccagag agatgttgtc gcggtttact tttttcacca cgccggtgat gatttcacct
    29701 tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg cttcacgcac tttctgcacg
    29761 ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg taacagactc aatctgatct
    29821 tcaacgtaat cgcccaggtt caggctttca tcttcataac gtgcggcttc aagggtgatt
    29881 tccttggtcg gctgggtgac ttcatcaaca actaaccagc gacggaaggt gtcaaaatca
    29941 ccgcttttgc gatcgatctg tacgcggacg tcgatctctt gttcatattt tttctttgtt
    30001 gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct cgcgaggtag cgccttttca
    30061 ttggatacgg cttcaactac agccaaaatt tctttgttca tcgcgggctt ttcacctcat
    30121 ccagactatt aaaagtgggg aaccaggttc gccttctgga tattactcag cgcgaacact
    30181 tcatctttac cttcgacggt aactgtgatc atttcaccgt ctaccgcttt gataacgccc
    30241 tgccatttac gacggttttg taccgccata cggagaacca gagtcacctc ttctccgaca
    30301 aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga gacccggtga ggagacttcc
    30361 aggttataag caacggtgat gggatcttca acatccagca cagcacttac ctggtggctc
    30421 acatcagcac aatcatcaac attgatgcca tcttcactat caatatagat gcgcagtgtg
    30481 gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa aacccagggc ctcaactggc
    30541 gcagtaatca tctctgttaa tttttgctct aatgtggaca agcccacccc caagacataa
    30601 aaaaagggct taaagcccag ttattctgta gtcagataac aaaaaacccc gataaatcgg
    30661 ggctttatat aactgaaccc tataaccgca actgcggtct ggagcacttt ccagaaggat
    30721 tttttcaaat cccactacga aggccgaagt cttcacagta tatttgaaaa aggactctaa
    30781 gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg agcccgacga
    30841 gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa ttctactcgt tttgggtaaa
    30901 agatgcaaat actgctggga tttggtgtac cgagacggga cgtaaaatct gcaggcatta
    30961 tagtgatcca tgccacattt tgtcaacgtt tattgctaat cacgtgaatg aatatccagt
    31021 tcactttcat ttgttgaata cttttgcctt ctcctgctct cccttaagcg cattatttta
    31081 caaaaaacac actaaactct tcctgtctcc gataaaagat gattaaatga aaactcattt
    31141 attttgcata aaaattcagt gagagcggaa atccaggctc atcatcagtt aattaagcag
    31201 ggtgttattt tatgacgacg attctcaagc atctcccggt aggtcaacgt attggtatcg
    31261 ctttttctgg cggtctggac accagtgccg cactgctgtg gatgcgacaa aagggagcgg
    31321 ttccttatgc atatactgca aacctgggcc agccagacga agaggattat gatgcgatcc
    31381 ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct gatcgactgc cgcaaacaac
    31441 tggtggccga aggtattgcc gctattcagt gtggcgcatt tcataacacc accggcggcc
    31501 tgacctattt caacacgacg ccgctgggcc gcgccgtgac tggtaccatg ctggttgctg
    31561 cgatgaaaga agatggcgtg aatatctggg gtgacggtag cacctataaa ggaaacgata
    31621 tcgaacgttt ctatcgttat ggtctgctga ccaatgctga actgcagatt tacaaaccgt
    31681 ggcttgatac tgactttatt gatgaactgg gcggccgtca tgagatgtct gaatttatga
    31741 ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc ctactccaca gactccaaca
    31801 tgcttggtgc aacgcacgaa gcgaaggatc tggaatacct caactccagc gtcaaaatcg
    31861 tcaacccaat tatgggcgtg aaattctggg atgagagcgt gaagatcccg gcagaagaag
    31921 tcacagtacg ctttgagcaa ggtcatccgg tagcgctgaa cggtaaaacc tttagcgacg
    31981 acgtagaaat gatgctggaa gctaaccgca tcggcggtcg tcacggcctg ggcatgagcg
    32041 accagattga aaaccgtatc atcgaagcga aaagccgtgg tatttacgag gctccgggga
    32101 tggcactgct acatatcgct tatgaacgtc tgttgaccgg tattcacaac gaagacacca
    32161 ttgagcagta tcatgcacat ggtcgtcagt tgggccgtct gctgtaccag gggcgttggt
    32221 ttgattccca ggcgctgatg ctgcgtgact ctctgcaacg ctgggttgcc agccagatca
    32281 ctggtgaagt taccctggag ctgcgccgtg ggaacgatta ttcaatcctg aataccgtct
    32341 cagagaacct gacctacaag ccagagcgtc tgacgatgga aaaaggcgac tcggtgttct
    32401 cgccagatga tcgtattggt caattgacca tgcgtaatct ggatatcact gatacccgag
    32461 agaaactttt tggttatgcc aaaactggcc tgctctcctc ctctgccact tcaggcgtgc
    32521 cgcaggtgga gaatatggaa aacaaagtta agtaatatca tagctttcgt gctaaaggcc
    32581 gcaaatgcgg ccttttttta cggctaagag aatgtgactg cgtttagggg ccaccatgtc
    32641 cgaattcgga attaaagtaa aagttaaatg aaataaagta aatttcagcg cattctattt
    32701 ttactttatg tttttatgct cagtaaaagg aatgaccgat gaatggtgaa tattttgtac
    32761 gcctcgcatt acatactctg aaatgcactc aaaaagattt agcaagccac ttagatgttt
    32821 cctcaacaca aattagcaag tggaaaaaaa ggggaacaca tgtccgctga tatggaaaaa
    32881 aagttcagaa atattacaca aataggagat tattctcctc aactagttga atggacgggc
    32941 tcagtcgcga atgctgagaa atgggatcga cttatccatt ttcttgctcg acgagctatg
    33001 gacgatgctg aaacgggtta tataacacaa cttcttactg acgaagatgg ttttttggtt
    33061 gaagaaacaa ttgatgtatt aaagcgaata ggttttccta cacctctttc ctttcctgaa
    33121 ggacttaata ttgacgacga taatgctgat gaagaagagg ctttttggga gatattagag
    33181 agcaatgctc attgttctgt aattaatgat atttatcatg ctctgaatga tgtttatggt
    33241 ttctacatag catatgttga tgagttgatt caggacgacg atttagatgt ctattcaagc
    33301 gaagccatca atattcagag ttcattgata tcacttgctg catgcaaaat tgaaattgat
    33361 actcctgttg cgtcaaactt taaagaattt cgttatagag ttaaaaaaga ctacgaaaat
    33421 tggttgaatc aactcaagat gatggcattc agggcgggaa ttccgttaag agccgaactt
    33481 ctggaaatgg tttataacac cgctgaccaa cttagcgttg cagcagaagc cgaacgtttc
    33541 gatttcaata aatctcgcat tcatccagat atatacatga acgagatcct tactggcatg
    33601 agaataattc accaagtgct tccggtaata atgcaaaagt tagaaattac agatttcaaa
    33661 ctagatgaaa cagatttatg cctgggtaaa tagttatcaa attaagaata cttagcctgg
    33721 cttataaaac gaaaaaagac gcttttcagc gtctcttttc tggaatttgg taccgaggac
    33781 gggacttgaa cccgtaagcc ctattgggca ctaccacctc aaggtagcgt gtctaccaat
    33841 tccaccacct cggcacggat actacttttt agttcgggat atcgctggtc ggcttagccg
    33901 gagcagctgg ttgagtttgt tcggttttca ccggtgcact cagattttcc cattcgctac
    33961 ctttattggt tttgttgcta ttgatgttac ccagcaccag actgatgatg aagaataacg
    34021 ttgccagcag cgccgtcatg cgggtcatga agttaccaga accacttgaa ccaaacagcg
    34081 tagcggaagc gcctgctccg aaggaggctc ccatatcagc gcctttacct tgctgcagca
    34141 tgatcagacc aacaaggcca attgccacaa taaggaaaac tactaaaaga gcttcgtaca
    34201 taatcaacct gttccttgcg gagttgccgc gtaccaatgc ttcaaccaat aaagcgggag
    34261 tttttaatct ttcccactga agcgggtgtg aatactaacc aaagcgaatg accttcgcaa
    34321 gggcaatttt atcgcattgt atcaactgcg gaaaaaaaca gcaaaaccga tgtgttcgct
    34381 gagaaaaaag gcgacaatcg ccgccttttt agccagttat ctaacgcttt aaacggcttt
    34441 tactgcatcg gcgatgcggt gtgcaaattc agtcacctgc gcttcgtctt cgccttccac
    34501 catcacgcga attaacggtt cggtgccgga tttacgcagc aacacgcgtc cacggttgcc
    34561 cagcgcagct tcaacctctg cggtcacggc tttaactgac tcatgctcaa gtggatcgcc
    34621 gctacctgcg gtgtaacgta cgttaaccag aatctgcggg aacattttca tgccgctgca
    34681 aaggtcgtgc aggctcatat ggttacgtgc catcgccgcc agcacctgca agccagcaac
    34741 gatgccgtca ccggtagtag ttttatccag caggatcaca tgaccggaat tctctgcacc
    34801 gatacgccag cctttctcct gcattttttc cagtacgtag cggtcaccca ctttcgcgcg
    34861 cgcaaatgga attcctaact gtttcagcgc cagttcaagc cccatgttgc tcatcaatgt
    34921 acccacagcg ccaccacgca gctggccctg acgaagacct tcacgcgcga tgatatacat
    34981 gatctgatcg ccatcgactt tattgccttc atggtcaacc ataatcacgc gatcgccgtc
    35041 gccgtcgaag gcaataccga gatccgcttt ttcagccagc acacgagcct ggagcgcgcg
    35101 aacgtcggta gcccccactt cggcattgat gtttacaccg tttggctcac aaccgatagc
    35161 gataacgttc gcccccagtt cgcgcagcac gttcggcgcg atgtgataag tcgcaccgtt
    35221 tgcacaatcc accacaatct tcagttcact gaggctaagt tcgttcggga atgtggcttt
    35281 gcaaaactcg atatagcgac ccgcggcatc aacgatacgg ctggctttac ccagttctgc
    35341 cgaatcaacg cagctgatct ccttttccat ttccgcttcg atggcctctt ctaccgcatc
    35401 cggcagtttg gtgccgtcga tagagaagaa tttaatccca ttatcgtaga acgggttatg
    35461 cgatgcagat atcacaattc cggcctctgc gcggaaggta cgcgtcagat aagccacggc
    35521 cggtgttggc atcgggccag tgaagagtgc ggaaaggccc gctgccgcca gacccgcttc
    35581 cagtgctgac tccagcatat agccagaaat acgcgtgtct ttaccaataa tgatcttacg
    35641 ggagccgtgg cgcgccagca ctttacccgc ggcccaaccc agcttaagca caaaatcagg
    35701 tgtgatcggc gcatccccta cacgaccacg aatcccatcg gtaccgaaat atttacgatt
    35761 actcatagcg tttgttttcc tttgcagaca gagtggcttc caccacccgc atcgcttcta
    35821 cggtttcttt gacgtcatga acacgaataa tgtgcgcgcc ttgcattgcg gcaatgaccg
    35881 cacaggccag actaccgctc aggcgctcgg acggccccac gttcagcagc tgcccaatca
    35941 tcgattttcg tgacatacct accaacagcg gcaggttgaa atggtgaaat tcagccaggc
    36001 gcgccagtaa tgaatagtta tgggagagat ttttaccgaa accgaatccg gggtcgagca
    36061 acaatttctc ttttgcgata cccgcctgct cgcaacgtgc tatttgctca ataaagtagc
    36121 gattcacttc tgcaaagaca tcgtcatact tcggagcttc ctgcatggtt tttggatttc
    36181 cctgcatatg catcagacaa accggtaaac cggtttctgc agccgcctcc agagcgccag
    36241 gttcggaaag ggagcggata tcgttaataa tgtgagcgcc aactttcgct gactcacgga
    36301 tgacttctgg tttggatgta tctaccgaga tccagacttc gaagcgttga gcaattgcct
    36361 caaccacagg aataacacgt tgcaactctt cttcaacgct aacttccgcc gcccctgggc
    36421 gcgtggactc gccaccaacg tcaatgatcg tcgcgccagc gttgatcatc agattcgcat
    36481 gtttcaccgc atctatcagc gagttatgcg tgccaccatc cgaaaaggaa tcaggcgtga
    36541 cgttgaggat ccccattacg tgaggatggc taaggtccag tgaagtgccc tgggcaaaga
    36601 gtttcatggt gttatccctg gtatgaattg ataagaaaaa accccggagc acgccccggg
    36661 gttttcggta caaatacagt catctgatgc gggaacttac ttgtcgccta actgctctga
    36721 catggtgtta cccgggttcg gcgtacgcgg ttcatcaacc ggacgaggag cctttggact
    36781 accattgtcg ccagaattgt tagaagcgcc tggttcttcc cagcccgctg gcggacgtac
    36841 atcgcgacgt gccatcaggt catcaatctg cggtgcgtcg atagtctcat atttcatgag
    36901 agcatctttc atcgcatgca gaatatccat attgtcggtc agaagctgac gcgcacgatt
    36961 atagttacgc tcaatcagtg ctttcacttc ctggtcgatg atacgtgcag tttcatcgga
    37021 catatgtttc gctttcgcta cgctacggcc gaggaacact tcaccttctt cttccgcgta
    37081 cagcagtgga cccaatttct cagagaagcc ccactgagtc accatgttac gcgccaggtt
    37141 ggtcgcaact ttaatatcgt tggacgcacc ggtagaaaca tgttccggcc cgtagatgat
    37201 ctcttctgcc agacgaccac cgtacagcgt agaaatctgg ctttccagtt tctgacggct
    37261 ggcgctgatt gcgtcgccct caggcaagaa gaaagtcaca cccagcgcac gaccgcgtgg
    37321 gataatcgtc actttgtgca ccggatcgtg ttccggcacc aggcgaccga taatcgcatg
    37381 acccgcttcg tggtaagccg tcgattcttt ctgcgcttcc gtcatcacca tggagcgacg
    37441 ttccgcaccc atcatgattt tatctttcgc tttctcgaac tcaaccatcg acacaacgcg
    37501 tttgttgcca cgagcagcga acagtgccgc ttcgtttacc aggttcgcca ggtcagcacc
    37561 ggagaaacca ggagtaccac gggcaatgat tgccgcgtcg atatcgggtg ccaatggtac
    37621 gcgacgcatg tgaactttca ggatctgctc acgaccgcga acatctggca agccaaccac
    37681 aacctgacgg tcgaaacggc caggacgcag cagggccggg tcaagaacgt ccggacggtt
    37741 agtcgcggcg ataacgatga taccttcgtt accttcgaag ccatccatct caaccagcat
    37801 ctggttcaga gtctgttcac gttcatcgtg accaccgccc agaccagcgc cacgctggcg
    37861 gcctacggcg tcgatttcat cgataaagat gatgcacggt gccgctttct tcgcctgttc
    37921 gaacatgtca cgaacacggg atgcacccac accgacgaac atttctacga agtcagaacc
    37981 ggagatagta aagaacggaa ctttcgcttc gcctgcaatc gctttcgcca gcagcgtttt
    38041 accggtaccc ggaggaccga ccatcaggac gcctttcggg atcttaccgc cgagtttctg
    38101 gaagcggctc ggctcgcgca gatattcaac cagttcagca acttcttctt ttgcttcgtc
    38161 gcagcccgca acgtcagcaa aggtcgtttt gatctgatct tccgtcagca tgcgcgcttt
    38221 gctcttacca aacgacatgg cacctttgcc accgccgccc tgcatttgac gcatgaagaa
    38281 gatccacaca ccaatcagca acagcatcgg gaaccaggag atgaagatag aagccagcag
    38341 gcttggttct tcaggcggtt caccgacaac cttgacgttc ttggtcaaca ggttatccag
    38401 taatttcgga tcctgaaccg gaatgtaagt ggtataacgg ttactatctt tcttggtaac
    38461 gttgatttca cgtccgttga tacgcgcttc acgaacctgg tcgttattga cctcttgtag
    38521 gaaggtagag taatccacct tacggccatt agactcgctg ggcccaaagc tctggaatac
    38581 tgacatcagc acaacggcaa tgaccagcca gagtattagg tttttcgcca tgtcactcaa
    38641 gggattaacc tcttattaca actgtgttaa aaacagcgtc aggatactct atatccagca
    38701 tctttcaaac tttcgtctga aatctcccgg ttagggttta cgcccggtcg ctacaatata
    38761 cacttcccgc gaacgtgcac gagaagagtc cggcttacga actttgacct tcgtaaacag
    38821 ggagcgaatt tcccttagat actcatcgaa accttcgccc tggaacacct tcactacaaa
    38881 actgccacct ggcgctaata catcacgaca catttctaac gccagttcca ccagatacat
    38941 ggcacggggg atatccaccg ccggtgttcc gctcatgttt ggtgccatat cggacatgac
    39001 aacctggact ttgctgtcgc caacgcgctc cagcagtgct ttcatcacca gttcatcacg
    39061 aaaatcgccc tgaagaaagt ccacaccaac gataggatcc ataggtaaaa gatcgcaagc
    39121 gatgatgcgg cctttgccgc caatttgggt gaccacatat tgtgaccaac cacccggagc
    39181 agcaccaagg tcgacaaccg tcattcccgg tttaaagagt ttgtcacttt gctgtatttc
    39241 atcaagttta aaccaggcac gggaacgtaa cccctttttc tgtgcctgtt gaacatattt
    39301 atcgctaaag tgttcctgaa gccagcggct ggagctggca gaacgcttct tacctgtcat
    39361 ttaactttcc catggggata actcatcgta accaattgcg taaattttta cgcgcctatt
    39421 tggtgatata tgggagatgg cggtagaatg acccgttttc aatcccaacg taagcaaaaa
    39481 atacgatgaa tctgagtact aaacaaaaac agcacctgaa aggtctggca catccgctca
    39541 agccagttgt tctgcttggc agtaatggtt tgaccgaagg ggtgctggcc gagattgaac
    39601 aagcgttaga gcaccatgaa ctcatcaagg tgaaaatcgc caccgaagat cgcgaaacta
    39661 aaaccttgat cgtggaagct atcgtgcgcg aaaccggcgc ctgtaatgta caggtcatcg
    39721 gtaaaacgct ggtgctttat cgcccaacta aagaacgtaa aatctcgctg ccacgctaag
    39781 attatcctaa agttacacac atttgctgtg taaaacgagg ggttttccgc aggcaggaga
    39841 gcaaaatgcc acgctctgtt cgttgataaa aggccgcata gcggcctttt tcctttcttt
    39901 acaatacatc aacatcttga gtattgggta attcttacag gtattccacc ttaattactt
    39961 caaattctac ttcgccgccc ggcgttttga tgaccacaac atcatcttct tctttgccga
    40021 tcaggccacg agcaataggc gagtttacag aaatcaggtt ttgtttaaag tcagcttcgt
    40081 catcgccaac gatgcgataa gtctgttctt cgtcagaatc cagattcagc accgttacgg
    40141 tagcaccaaa aataacgcgc ccattgttgg gcattttggt gacatcaatc acctgcgcgt
    40201 tcgacagctt ggcttcgatg tctttaatac ggccttcgca gaaaccctgc tgttcacgag
    40261 ctgcgtggta ttcggcgttt tctttcaggt cgccatgctc acgcgcttcc gcgatagcag
    40321 caatgatttc aggacggcgc acagatttca gaaaatccag ctcttcgcgt aatttttcag
    40381 cgccgcgtaa ggtcatcgga atagcttgca tttgttatac ctcttgaata ttcctgatgg
    40441 ggcaagtctt cacccacccc ggctgttcgg cctgcccggc ataactcctc accgggacca
    40501 gaagcaaaaa aataccgacc cgggtacaag tcccaggtca gctacaattc acattttgat
    40561 agtcatttta ccctgaagtt cccgaagggt catcgtttac tttatagggc gttgcgccgt
    40621 agtatgacgg ctcgattcca ggttgttagc gcgagattat gcgattttcc agatttatca
    40681 tcggattgac cagctgtata gcgttcagtg ttcaggccgc gaatgttgat gagtacgtta
    40741 ctcaactccc cgctggtgcc aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc
    40801 ccgctattga ttaccacagt cagcagatgg cgctgcccgc cagtacgcag aaagtgatta
    40861 ctgcgctggc ggcgttgatt caactcggcc ccgattttcg tttcaccacg acgcttgaaa
    40921 ccaaaggcaa tgtggaaaac ggcgtactta agggtgactt agtggcgcga tttggtgccg
    40981 atccgacgtt aaaacgtcag gatattcgca atatggtcgc gactttgaaa aaatctggcg
    41041 tcaaccaaat cgatggcaat gtgttgatag atacctccat tttcgccagc cacgataaag
    41101 cccccggctg gccatggaat gacatgacac aatgctttag cgctccgcct gccgctgcca
    41161 tagttgaccg caactgtttc tccgtctcgc tctacagtgc cccaaaacct ggtgatatgg
    41221 cttttatacg cgtggcatct tattaccccg ttacgatgtt cagccaggta cgcaccctcc
    41281 cccgaggttc tgccgaagcg caatactgcg aactggatgt cgtaccaggc gacctgaacc
    41341 gctttacgct gacgggatgc ctgccacaac gttctgagcc gctcccgttg gcttttgccg
    41401 tgcaggatgg agccagctat gccggggcaa ttctgaaaga tgagttaaaa caggcaggta
    41461 tcacctggag cggaacactg ctgcgccaga ctcaggttaa cgaacctgga acggtagttg
    41521 ccagtaaaca gtcggccccg ttgcacgatc tgcttaagat tatgctgaaa aagtcggaca
    41581 acatgatcgc cgatacggtt ttccgcatga taggccatgc gcgcttcaat gtgcctggaa
    41641 catggcgggc cgggtcggac gccgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata
    41701 ttggaaacac cattattgcc gatggttcag ggctttcgcg gcataacctg attgcccccg
    41761 ccaccatgat gcaggtgttg caatacattg cccaacacga caatgaactt aactttatct
    41821 ccatgctgcc gctggcgggc tatgacggct ctttgcagta ccgtgcaggt ctgcatcagg
    41881 cgggcgtgga tggaaaagtc tcagcgaaaa ccggttcgtt gcagggggta tataacctgg
    41941 cgggattcat taccacagcg agcgggcaac gaatggcgtt tgtacaatat ctttctggct
    42001 atgcagtaga accggcggat cagcgtaatc gccgtattcc gttagtgcgt tttgaaagcc
    42061 gtttgtataa agatatttat cagaacaatt agtcaaaaag aaaccccggc acatggccgg
    42121 ggttttgctg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag
    42181 tcatcatccc agtcctcatc atcctcttca gcaatctctt caagctgctg gcgatgataa
    42241 tcatcccaca tgaattcgac tttctctggc tgtttcgctt cttcagcctg cacgaccggg
    42301 ttttcaatga taaaggtcat cacatcccag cagagatctt tcacacccag tccactcgcc
    42361 gcagagatca gataatattt atcttcccag cccagcgctt cagcaatcgc tttcgctttc
    42421 tcttcagctt ctgccttatc cagcagatcg atcttattga acactaacca acgcggtttc
    42481 gccgccagat cctggctgta tttttccagc tcgctgataa taatacgcgc gttttcaacc
    42541 ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca acaggacgcg gcaacgttcc
    42601 aggtgcttca ggaagcggat acccagacct gcgccttccg cagcgccttc aatcagtcct
    42661 ggaatatcgg caacaacgaa gctcttttcg ttgtccattc gtaccacacc cagacttggc
    42721 accagagtgg taaacggata atccgccact ttcggtttag ccgccgatac cgcgcgaata
    42781 aaggtcgatt tacccgcgtt tggcatcccc aacataccga cgtcagccag cagcatcagc
    42841 tccagcagca gctcacgctt atcacccggc gtaccgttgg ttttctgccg tggtgtacgg
    42901 ttaacggacg atttgaaacg ggtattgccc agaccgtgcc agccgccctt agcaaccagc
    42961 agacgctgac cgtgtttagt catatcgccc atggtttcac cagtaccttg gtcgattata
    43021 cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc gcttaccggt acaatcgcgg
    43081 cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt caaaacgata atcgataagc
    43141 gtgttcaggt tttcgtcggc ttccatccat acgtcaccac catcaccacc gtcgccgcca
    43201 tccgggccgc ctttcggaat atacttttcg cggcggaagc tcacgcaacc attaccgcca
    43261 tcacctgcaa cgaccagaat cgatgcttca tcaacaaact tcattttatt ctccgtaaat
    43321 cattcgcctg cgcggggttg cgataccacc gttgtatgct tacgtaatcc gccccaaata
    43381 cgatgaccaa tggcggaata catcgcgccc gcaaccacga caaacgcacc gagataacct
    43441 aaaaggttta acatcggtct ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa
    43501 aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag
    43561 cgagccatcg cttccgccag ggcgccatat cctaccaatg tattcagtcc gcaaaaaatt
    43621 aaacatgcga gctgccagtg gctaagctgc gctatcactc caggctttgc cagagggaag
    43681 agcgcaattg tacataaagc gtacagtaaa aacaggatct gcggtgaggc cagccgacgc
    43741 aataaaacct tttgcgccac gccataactc acccaaaccg tcgccgcacc gaccccaaag
    43801 ataactcccc aggtgtaatc ggtgagcttt gtaaatatct cgaccagact ggtgttaaaa
    43861 aacatcacca ggccgctcag gagcatcaat gcccctacaa cctgagtgct gcgcattttc
    43921 tctttcagga taaatacgct ggcaaccatc atgccaactg gcgagagttg cccaatcacc
    43981 tgcgaagcgg tcggactcag gtattgcaag gatgagctga acaggatgaa gttcccaaac
    44041 agcccggcgg tcgccactgc caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat
    44101 ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc caatactcgc catcaagaaa
    44161 cggtaaaaca cgattgtcgg aggttccatc acctccagca cctgcttcat tgcgattggc
    44221 aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc tgcctgctgc
    44281 ttcataccgt attccctgca aagcgcattt gcggattaac gtcgcaccat gcgcattttt
    44341 accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg
    44401 acgcgaaaaa cttattcagc ttcgatgctg ataaatttac ggtttttcgg gcctttaact
    44461 tcgaatttca ctttaccgtc tgctttagca aacagagtgt ggtcacgacc gcaacctacg
    44521 ttagcgccag cgtggaattt ggtaccacgt tgacgaacga tgatgctacc cgccagaacg
    44581 gattcgccac cgaaacgctt aacgcccagg cgtttagctt ctgaatcgcg accgttacgt
    44641 gtggagccgc cagccttttt atgtgccatt tgaaatctct cctcaggtct taggcgctga
    44701 tgccagtaat tttcacatca gtgaaccact gacgatggcc ctgctgctta cgatagtgtt
    44761 tacgacgacg aaacttaacg attttaactt tctcgccacg accgtgagca acaacttcag
    44821 ctttgattac gccgccatca acgaaaggaa cgccgatttt gacttcttca ccgtttgcga
    44881 tcatcagcac ttcagcgaac tcaacagttt cgccagttgc gatgtccagc ttttccaggc
    44941 gaacggtctg accttcgctt actcggtgtt gtttaccacc actttggaaa accgcgtaca
    45001 tataaaactc cgcttccgcg cacacctttt caatgattca gagtgcgcta taaatattca
    45061 caatagggcg cgaatattac gcaaaacgca cgcctttgac aagtgctaca gtcaatacac
    45121 gaagaaaaaa aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatc
    45181 accctatatt cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc
    45241 gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat
    45301 ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca
    45361 gttaggctat tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact
    45421 ggctgcacga gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga
    45481 gtttatccac acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag
    45541 gggtaaagct accgccaacg ctgcatttgg caatgccgcc agcgtgctgg taggcgattt
    45601 tatttatacc cgcgcgttcc agatgatgac cagcctcggt tcgctcaaag tgctggaagt
    45661 catgtcagaa gccgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa
    45721 cgatccggac atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct
    45781 gtttgaggct gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa
    45841 aggcctgcag gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact
    45901 cgattacaat gccgatggcg aacagttagg taaaaatgtc ggcgacgatc tgaacgaagg
    45961 taaaccgacg ctgccgctgc tgcacgcgat gcatcatggc acaccagaac aggcacagat
    46021 gatccgtacc gccatcgaac agggtaacgg tcgccatctt ctggaaccgg ttctggaagc
    46081 aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt gccgaggaag aagcagacaa
    46141 agccatcgca gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct
    46201 cgcgcacatc gctgttcaac gcgatcgtta atcccctccc ctcatcccgc gcagcgcgcg
    46261 ggatggttcc agtaaattcc ataaaaacac ttattcagct ctataacacc cgaaaaacag
    46321 ggctgtgcat ctttatatat tctgaatatt cacactcttt acaggagctt tttagagcaa
    46381 taggccatca ggagtatagt gatgctcgac agaagaagtg ttctgaatga aagcgactaa
    46441 cttaaggagt gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt
    46501 gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt ctcgcagaaa tggtttgagt
    46561 tcctcaacgc tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg
    46621 aaagccctgg gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc
    46681 catgagttta tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg
    46741 gtctggcggt agccccgcga acggggctgc cagctcttag acgattactc gcctttcaca
    46801 cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg
    46861 atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa tacaaccagc cagcaccagg
    46921 cttgctgatg cacgcagatc ggttgccata acctgtgcgc cagaaagttt ttcaacaccg
    46981 tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc ccatacggct aagctccggc
    47041 acatgcataa agcggttttc aaagaccgtt tcggtgatga atccggtccc ttctgccacc
    47101 aggttcaaca gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta
    47161 cgtacgttaa cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg
    47221 acttcgatgt ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag agtatctggc
    47281 tgcgcgttac ggcagataat tttgccgcga gaaatcgccg ccgccaccag gaaagtaccg
    47341 gtttcgatac gatccggtag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg
    47401 atgacgatac gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc
    47461 gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt accttccgcc
    47521 agggttgcag cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata
    47581 tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg
    47641 gtcgcgccta attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta
    47701 caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagtgcc
    47761 cagatagaag cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg
    47821 cgggcatcaa tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagc
    47881 ttcattgatg tatcgacgtc tttcagtttt gggacgttct ggatctctac cggttcttcc
    47941 gccagtagag cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact
    48001 tcgccctgga gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt
    48061 taacaattca tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga
    48121 tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc
    48181 gcaatatatt ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcatgccg
    48241 tcaaacaact cacccacggc aataacctga aagtggctgc catcgtcgga aacgtggact
    48301 tcctggaggg agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct
    48361 tcaatcatca gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata
    48421 agcaaaaagc ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca
    48481 ggcggttaac gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa
    48541 ttatacaatt tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacattgttg
    48601 ccctgctttt tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg
    48661 cgggacacac ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc
    48721 caaagcggta gcaaaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc
    48781 tgcatccagc tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag
    48841 aaatcgattt cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc
    48901 actcgttttg tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc
    48961 agttgcccgt ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat
    49021 tcggatcaat aatggtaacg cgaatcggca caatggtttt atcgcccagc ggctgttctg
    49081 gcgcaatctg ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca
    49141 ggtactcacg gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat
    49201 actggcccag caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat
    49261 caacaatggt acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc
    49321 gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg
    49381 cggttgccgc actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca
    49441 tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac
    49501 ctggcgcagc agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa
    49561 ggaactgacc aatgagatct tccagcacca tcgcagactt agtgtcctga attgtatcgc
    49621 catccttcag gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat
    49681 attgttcccc cagcaggccg gaagtacgaa tgctcagcga actggtatct ggaatgtggt
    49741 tataacgttg ttcaatttcc agcgttacgc gtggcagata ggttttcggg tccagcgtaa
    49801 tatccgccac ccggcccaca acaacgccac caatactgac cggagagcgc gctttcaggc
    49861 cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt atggacgtca
    49921 cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac
    49981 ccacccaaat ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc
    50041 agcacaaaat ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc
    50101 cggctaatcc cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc
    50161 accgtgatgg cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg
    50221 acggcatttt gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg
    50281 accagagatc cgccccagat ccccacggcg acgaaaataa ccgtcaacag tggtaatgaa
    50341 ataaccccag cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc
    50401 tccatactgg agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa
    50461 ccagcacgcc cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat
    50521 aacgccacca gcatacccag actggtttcc gcactataag tggtcagaac cagataacct
    50581 tgcagcccca acaccattcc gatgaacacg ccagaaacta caataatcag cattgacagg
    50641 acgccgacat tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg
    50701 ccgaccagcg cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc
    50761 cctttatgtc cgagcgacgc cagcacattt aacagcatga gtggcttaac tccctggtaa
    50821 aagatcagcg tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc
    50881 gtccagaaac tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cactgccatg
    50941 agcgacaatt tttttgtccg ccaggatcca ggcatgatcc gcaatactta acacttccgg
    51001 cacatcgtga gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag
    51061 cttcaccagt acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat
    51121 gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga
    51181 aagttcagaa ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat
    51241 caccgtacta tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac
    51301 gttgtcaaat acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg
    51361 tttgcgcact gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag
    51421 gatctcacca tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc
    51481 gatgcccgat ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt
    51541 atcgaagatg cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc
    51601 cacagactgc tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa
    51661 tttaaccttg aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc
    51721 atttgtttta ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa
    51781 aatcttcagc ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg
    51841 cactgttaat tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg
    51901 cgtctattct ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca
    51961 gtattggtac atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac
    52021 gcgatttagc cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg
    52081 gtctggctgc gctggttcgt ccttttaccg tccattctga tgttctacgc cgtgaattac
    52141 ccttaatgtt gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc
    52201 gcagcgatgg tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac
    52261 ttgcacgtca ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc
    52321 tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca
    52381 tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca
    52441 tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg
    52501 caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg
    52561 gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag
    52621 agattgatcc attggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt
    52681 ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa
    52741 ctggcggatt tatcgtatgg ctggcgattt tgtactggtt atcgccaata ctcgttgaat
    52801 aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca
    52861 ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat
    52921 cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg
    52981 gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt
    53041 acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc
    53101 ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta
    53161 attccagtgc ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc
    53221 agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg
    53281 ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct
    53341 gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc
    53401 ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag
    53461 atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt tgctggaagt tacccgcaaa
    53521 aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac
    53581 ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat
    53641 gtgatgacgc cggggggaat acgtgtgcgc cctggcattc tggccgttga ggcactgaac
    53701 ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt
    53761 gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa
    53821 caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta
    53881 tagcaaaagc agagaacatt cgtctgctga tcctcgatgt cgatggcgta ctgtcagatg
    53941 gcctgattta tatgggcaat aatggcgaag agctgaaagc gttcaatgtt cgtgacggtt
    54001 atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg
    54061 ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt
    54121 caaacaaact gatcgccttt agcgatctgc tggaaaaatt ggcgattgcc ccggaaaatg
    54181 tggcttatgt cggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg
    54241 tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg
    54301 ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt actcctggcg cagggcaaac
    54361 tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct
    54421 atcactggcg gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca
    54481 ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta
    54541 taacccagaa ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga
    54601 tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc
    54661 gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata
    54721 tggacacgtt gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa tcacgacgga
    54781 taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata
    54841 cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc
    54901 cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta
    54961 atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag
    55021 acactgatca gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg
    55081 tggttacctt taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca
    55141 aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggctacggta
    55201 aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc
    55261 agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc
    55321 aggtcgatag caacattaag ggcgataaga tcacttacct ggtaaaagag cagaaaatgc
    55381 aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg
    55441 acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa
    55501 cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca
    55561 gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga
    55621 ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg
    55681 atgatgacga tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc
    55741 tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac
    55801 tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg
    55861 aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac
    55921 gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg
    55981 aaccgtttgc cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgagcacc
    56041 tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg
    56101 tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag
    56161 aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat
    56221 agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt
    56281 gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct
    56341 gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc
    56401 gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga
    56461 aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga
    56521 tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat
    56581 tgacgatgag ctgccggtct atcagggcga aacgacgcag accttgcagg attacctgat
    56641 gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt
    56701 cgatgccgtt gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat
    56761 gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt
    56821 tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca
    56881 attcgataaa accacgccgt ggctggaaga ggccagactg atcattagcg atcatctcga
    56941 tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt
    57001 gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca
    57061 gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg
    57121 gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc
    57181 gatgtgcaat aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga
    57241 tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg
    57301 ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat
    57361 ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac
    57421 cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag
    57481 tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cgctggtgaa
    57541 gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt
    57601 gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt
    57661 atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca
    57721 ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg
    57781 ttacagccaa atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg
    57841 ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg
    57901 aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata
    57961 agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc
    58021 aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc
    58081 tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg
    58141 atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact
    58201 gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagtc
    58261 tgccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta
    58321 tcggcaatgg tattgccatt ccgcatggca aactggaaga agatactctg cgcgccgttg
    58381 gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg
    58441 atttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt
    58501 cactggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga
    58561 gcgatgaaga gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt
    58621 tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg
    58681 tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt
    58741 tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg
    58801 atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa
    58861 tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaactc ctgttcctgg
    58921 atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt
    58981 ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggaacctc
    59041 tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg
    59101 aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgacaatg gtctttgagt
    59161 ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct
    59221 tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat aagcccgtcg
    59281 ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc
    59341 ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg
    59401 gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc
    59461 gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac
    59521 cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa
    59581 gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac
    59641 cgaggctgaa gccaatagcg ttattgcact gctgatgctg gattctgcca aaggacggca
    59701 gattgaagtt gaagccaccg gtccacagga agaggaagca ctggccgccg ttatcgccct
    59761 ctttaattct ggttttgatg aagattaatc ttcatcactt tgacattcaa atcccttcaa
    59821 gctcccccga caataagaaa agcgcgtact gaaatcgttc tcaatcaacg tcatttgtac
    59881 attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg cattgtttag
    59941 gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc aggagatggc
    60001 atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg
    60061 catccggaag atgcccaacg ctgtattaag attgtctacc atcgtggcga tggtggcgat
    60121 aaagagatcc gccgtgagtt aaagtattac gcgcatcttg gtcgccggtt aaaagactgg
    60181 agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac
    60241 gatgtgattg ccgatttcga cggcaatccc tccattacgc tgaccgaatt tgctgaacaa
    60301 tgccgttacg aagaagacgt tgcacaactg cgccagttac tgaaacagct gaagcgttat
    60361 ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcatcgc
    60421 atcagtgagt cagaggttat ccccgtcgtc tgcgacaaca ttggtgaaag cacattgatt
    60481 ccactggcaa cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga
    60541 tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc
    60601 aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgctgttg cataaacgac
    60661 tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg cacgtagccc
    60721 gatggcgcgg agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct
    60781 tccgatcggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg
    60841 ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg
    60901 ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg aacccagctg
    60961 cgcccatccc ataaaaagag atttttggct gtctgctgag aaatcgttga agcaccgcga
    61021 atacggtttt cattgcgctc gttgtgcgcc agggctttct caatggaagc aacatcaaag
    61081 ccccagtgct caggaaattt ctgatcttct gcggcaatca ccgccagtcc catccatggc
    61141 gagatttgat ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc
    61201 caggcgctga cctgtcgctc gaccattacc gctgagaagg gaacaggcgc aacactaaac
    61261 aacgcgatgc ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa aagaaaacgg
    61321 cgaacgaaac taaacaccgt taagcggctt ttactcattc agccagaacc agcacgcggg
    61381 aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc atatatgctg
    61441 gggtggtgac aatcttattg tcttcatcaa ccacgatatc atcgacagga cagggcacat
    61501 gctccgcgcc catctcttcc agcacttctg cggtatcgat atcagtaccg atggtcaaac
    61561 gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc aatacacata aaaccaagcg
    61621 gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt
    61681 cgctaccaag agtggcaaaa ttgcttaaat tcttcgccgc gccaaacccc cccggcacaa
    61741 tcaacgcatc cagttcagcg gcatcggcct gggccagagg acggatttca ccgcgcgtta
    61801 ttcgtgccgc ttcaatcagc acgttgcgcg tttccgtcat cgcttcgcca gttaaatggt
    61861 tgataacatc aacctgctgc ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg
    61921 aaatagctaa caacgtcaac accgcttcat gaatttcaga accgtcatag acgccgcatc
    61981 cgctcagaat tacgccaatt ttcttcattg tgatcatcct ttcgcaattg actgaaacac
    62041 attaattttt ttaataaaaa tggtacgcat cacacattta actgattcat gtaacaaatc
    62101 atttaagttt tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac
    62161 tcatcataat aatttacggc gcagccaaga tttccctggt gttggcgcag tattcgcgca
    62221 ccccggtcta gccggggtca ttttttagtg gcttttgcca cccacgcttt cagcacttct
    62281 acgtcgtgac gccactcttc tttcatctct tcaatccatt caccgacgtt atcttcccag
    62341 gcaggaaggt caggagactg aatttgctga cccagctgtt gcagatggcg taaccccact
    62401 gaccccgccg cacctttaat tttatgtcct tcctcaacaa tgcctttttt atcctgcgcc
    62461 gtcagattcg actccagcac gctgacatag cccggcatca tcttctcaaa caccgctaac
    62521 ccgtcggtga tcagcttcgg tcctacaagt tcgagatact gttccagcat gggaatatcg
    62581 agcaatgctt ctgatttact gttctcttct gtcgtcaccg tactctcctc atcatcctgg
    62641 gtatcccaga atttcttgat catcgcggtt agcgccggaa ccgaaagcgg cttactcagc
    62701 acatcatcca ttccagcatt gaggtactct tgtttgtctt tcagcacgtt agcggttaag
    62761 gccaccagcg gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg agagatatcc
    62821 agcccggtca tatctggcaa ctgaatatcc agcaacacca ggtcgtattc gcccggttta
    62881 aacatctcca gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc taatttttcc
    62941 agcacagaac gcgcaacaat cacgttcagt tcaatgtctt ccaccagcag cacattcagc
    63001 gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc taccgacggt
    63061 gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg
    63121 ccgcccatat ttttcgccag acgacgagaa acggccagac caataccggt gccggtggca
    63181 ggtttaccgc catgactgtc tttcacctgg taatacatgg cgaaaatttt atccagctca
    63241 tcctgcggaa tgccgatacc agagtcttcc acttcaaaat gcagcatatc gccttcatcg
    63301 tagcgcacac gcacggtaac caggccttgc tgggtgaatt tgacggcgtt actgatgagg
    63361 ttccacagga tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg taatggcagc
    63421 gtcggctcca ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga gagattttcc
    63481 agatcggcaa ggaagctggt gaaatcaacc ggttgattat caagctggac cttgcgccgt
    63541 tccatcttat ccatgtcgat aatatcgtta aagatattcc ccagcgtgac ggccgaaaca
    63601 tggatggtct tgagatattt ttcctgctcg gcggtgagtt cggtatccag cagaatgcgg
    63661 ctcagaccga cgataccgtt cagcggtgta cgcaattcgt gactgatggt ggagataaac
    63721 gtcgttttgt cgcggctggc ccgttcaagc gcatcctgat accgcttacg ctcggtaatg
    63781 tcgcgaccaa agcccatcaa accgtgacgt ttacccacgc ggtcgtagta cggcacttta
    63841 cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca gccactgttc ataggtcagt
    63901 gacacattat gacggaacac tttttcatcg gtttcaatga cttttgcggc ggcttccggt
    63961 gagtaaacat cagcaggttt caggtgaacc agttgttttt cgctttttcc ggtcagcagc
    64021 tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg
    64081 ggtgaagcat caaggaagga acgtaagaat gaggattgct gctcgagctg aatttgtgtc
    64141 tcttcgcgct ctttgatttc aattttcagt tggccgaagg tttcctgcag ttctgcttcc
    64201 gctttttcac gaacggcaat ttcctgattt agctgggcaa tattatcttt taactgaacg
    64261 ttgaggctca aatcgcgctc gcgcatctcc tccagttttt gcaccagccg tgacagacgt
    64321 tgtcgtgact cctccagttg ctcgacgacc accgatagaa agtagaccgc ccacggcgta
    64381 atcagcaaac caaagaagat agaacgaata acatcaatgc tttcgacctg accatgcagc
    64441 accatggtta ccgccatttg taccacaatg gcaagaacga cgagggccag cgccagcaac
    64501 attgagaagc gcaccagacc taacttcatc atcaggtcaa cataatactg cgccagcaga
    64561 cgaatttgct tcattaggga attccttcac gacaacctgc cccaataata cccaattctg
    64621 acagttacgt tataaattgt gtgagaaatg cggaattccc ctcaacgacc tactccgttg
    64681 gaggtagcca cggacgtccc agcgccgatc cctgtacgcc atgttcattc agatagcgat
    64741 ccagctcgac cattcccgtc catcgatttt cgcaccacag aggagcaagc agcgtcgggc
    64801 gacgggcgct ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg cgaatcattt
    64861 ctccggcagt gagcgtgtaa tcctccagtt caataccgtt caaacgcccc gcttcccagg
    64921 ctttcgccat aatgctgcct ttcacaatat gcagcggatg cagctttatg ccgtccacgc
    64981 cggtttcaac cactcgctcc agcgtttgca aacattcagc ctgcccttcg ccaggcaggc
    65041 cgacaattaa gtgggaacac accttcagcc cgcgctgacg tgccagctgg gttgtacgct
    65101 gataacaggc aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg tgggcggttt
    65161 gtagccccag ctccagccac acttcgtaac cctggtcctt atattcgcaa agcagatcca
    65221 gcaccgcatc cggcacgcag tccgggcggg taccaacaca caaaccgaca atattggcct
    65281 ggctcaccgc ctgctgatac atagaacgca gcacctgaac ttccgcaaag gtgctggtat
    65341 acgcctgaaa gtaggccaga tagcgtttag cgcggttaac taaattcgcc tggtgcgcca
    65401 gttgctcggc aatggaacga tgctgctgcg cttcatcggc aaacgaggca acattacaga
    65461 atgtgcagcc accacgcccg atggtaccgt cacggttagg gcagctaaaa ccgccgtgta
    65521 gcgtcagctt atgcaccttt tgcccataac gacgggtgag atcaccacca aacatattga
    65581 ctaatttctg taactgcata atctgataga ccgcgccttg aaaagaggcc aaagcctgcc
    65641 atttttagct caattcggcg atgacctgga tcaatcgtcc cggcctgctt ttatcaactg
    65701 cataatcaat caaaattacc gaaatttcat gc
//
LOCUS       DAZFAH010000004        65718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFAH010000004 DAZFAH010000000
VERSION     DAZFAH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.004"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..910)
                     /locus_tag="ACHJWB_000196"
     CDS             complement(<1..910)
                     /locus_tag="ACHJWB_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4311898.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQLLSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSST"
     gene            1246..2448
                     /gene="nupC"
                     /locus_tag="ACHJWB_000197"
     CDS             1246..2448
                     /gene="nupC"
                     /locus_tag="ACHJWB_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4311899.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2498..4687)
                     /gene="pdeA"
                     /locus_tag="ACHJWB_000198"
     CDS             complement(2498..4687)
                     /gene="pdeA"
                     /locus_tag="ACHJWB_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4311900.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4895..4970)
                     /locus_tag="ACHJWB_000199"
     tRNA            complement(4895..4970)
                     /locus_tag="ACHJWB_000199"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4935..4937),aa:Ala,seq:ggc)
     gene            complement(5010..5085)
                     /locus_tag="ACHJWB_000200"
     tRNA            complement(5010..5085)
                     /locus_tag="ACHJWB_000200"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5050..5052),aa:Ala,seq:ggc)
     gene            5306..5665
                     /gene="yfeC"
                     /locus_tag="ACHJWB_000201"
     CDS             5306..5665
                     /gene="yfeC"
                     /locus_tag="ACHJWB_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4311901.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5667..6059
                     /gene="yfeD"
                     /locus_tag="ACHJWB_000202"
     CDS             5667..6059
                     /gene="yfeD"
                     /locus_tag="ACHJWB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4311902.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6111..7526)
                     /gene="gltX"
                     /locus_tag="ACHJWB_000203"
     CDS             complement(6111..7526)
                     /gene="gltX"
                     /locus_tag="ACHJWB_000203"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4311903.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7785..7860
                     /locus_tag="ACHJWB_000204"
     tRNA            7785..7860
                     /locus_tag="ACHJWB_000204"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7818..7820,aa:Val,seq:tac)
     gene            7905..7980
                     /locus_tag="ACHJWB_000205"
     tRNA            7905..7980
                     /locus_tag="ACHJWB_000205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7938..7940,aa:Val,seq:tac)
     gene            8027..8102
                     /locus_tag="ACHJWB_000206"
     tRNA            8027..8102
                     /locus_tag="ACHJWB_000206"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8060..8062,aa:Val,seq:tac)
     gene            8107..8182
                     /locus_tag="ACHJWB_000207"
     tRNA            8107..8182
                     /locus_tag="ACHJWB_000207"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8140..8142,aa:Lys,seq:ttt)
     gene            8213..8335
                     /locus_tag="ACHJWB_000208"
     ncRNA           8213..8335
                     /ncRNA_class="other"
                     /locus_tag="ACHJWB_000208"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8455..8796
                     /locus_tag="ACHJWB_000209"
     CDS             8455..8796
                     /locus_tag="ACHJWB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4311904.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(8787..9713)
                     /gene="yfeR"
                     /locus_tag="ACHJWB_000210"
     CDS             complement(8787..9713)
                     /gene="yfeR"
                     /locus_tag="ACHJWB_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4311905.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            9803..10801
                     /gene="yfeH"
                     /locus_tag="ACHJWB_000211"
     CDS             9803..10801
                     /gene="yfeH"
                     /locus_tag="ACHJWB_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4311906.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(10798..11016)
                     /gene="ypeB"
                     /locus_tag="ACHJWB_000212"
     CDS             complement(10798..11016)
                     /gene="ypeB"
                     /locus_tag="ACHJWB_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4311907.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(11018..13033)
                     /gene="ligA"
                     /locus_tag="ACHJWB_000213"
     CDS             complement(11018..13033)
                     /gene="ligA"
                     /locus_tag="ACHJWB_000213"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4311908.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGRLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(13104..14090)
                     /gene="zipA"
                     /locus_tag="ACHJWB_000214"
     CDS             complement(13104..14090)
                     /gene="zipA"
                     /locus_tag="ACHJWB_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4311909.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGIFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            14320..15081
                     /gene="cysZ"
                     /locus_tag="ACHJWB_000215"
     CDS             14320..15081
                     /gene="cysZ"
                     /locus_tag="ACHJWB_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4311910.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            15266..16237
                     /gene="cysK"
                     /locus_tag="ACHJWB_000216"
     CDS             15266..16237
                     /gene="cysK"
                     /locus_tag="ACHJWB_000216"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4311911.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            16621..16878
                     /gene="ptsH"
                     /locus_tag="ACHJWB_000217"
     CDS             16621..16878
                     /gene="ptsH"
                     /locus_tag="ACHJWB_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4311912.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            16923..18650
                     /gene="ptsI"
                     /locus_tag="ACHJWB_000218"
     CDS             16923..18650
                     /gene="ptsI"
                     /locus_tag="ACHJWB_000218"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4311913.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            18691..19200
                     /gene="crr"
                     /locus_tag="ACHJWB_000219"
     CDS             18691..19200
                     /gene="crr"
                     /locus_tag="ACHJWB_000219"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4311914.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(19242..20093)
                     /gene="pdxK"
                     /locus_tag="ACHJWB_000220"
                     /gene_synonym="thiJ"
     CDS             complement(19242..20093)
                     /gene="pdxK"
                     /locus_tag="ACHJWB_000220"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4311915.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            20198..20566
                     /gene="yfeK"
                     /locus_tag="ACHJWB_000221"
     CDS             20198..20566
                     /gene="yfeK"
                     /locus_tag="ACHJWB_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO4311916.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(20569..21480)
                     /gene="cysM"
                     /locus_tag="ACHJWB_000222"
     CDS             complement(20569..21480)
                     /gene="cysM"
                     /locus_tag="ACHJWB_000222"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4311917.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(21614..22711)
                     /gene="cysA"
                     /locus_tag="ACHJWB_000223"
     CDS             complement(21614..22711)
                     /gene="cysA"
                     /locus_tag="ACHJWB_000223"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4311918.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(22701..23576)
                     /gene="cysW"
                     /locus_tag="ACHJWB_000224"
     CDS             complement(22701..23576)
                     /gene="cysW"
                     /locus_tag="ACHJWB_000224"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4311919.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(23576..24409)
                     /gene="cysT"
                     /locus_tag="ACHJWB_000225"
     CDS             complement(23576..24409)
                     /gene="cysT"
                     /locus_tag="ACHJWB_000225"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4311920.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(24409..25425)
                     /gene="cysP"
                     /locus_tag="ACHJWB_000226"
     CDS             complement(24409..25425)
                     /gene="cysP"
                     /locus_tag="ACHJWB_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4311921.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(25596..26387)
                     /gene="ucpA"
                     /locus_tag="ACHJWB_000227"
     CDS             complement(25596..26387)
                     /gene="ucpA"
                     /locus_tag="ACHJWB_000227"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4311922.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(26516..27373)
                     /gene="murR"
                     /locus_tag="ACHJWB_000228"
     CDS             complement(26516..27373)
                     /gene="murR"
                     /locus_tag="ACHJWB_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4311923.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            27537..28433
                     /gene="murQ"
                     /locus_tag="ACHJWB_000229"
     CDS             27537..28433
                     /gene="murQ"
                     /locus_tag="ACHJWB_000229"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4311924.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCWIINDEDKTVPLAVERV
                     LPDIAASIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            28437..29861
                     /gene="murP"
                     /locus_tag="ACHJWB_000230"
     CDS             28437..29861
                     /gene="murP"
                     /locus_tag="ACHJWB_000230"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4311925.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            29866..31170
                     /gene="pbp4b"
                     /locus_tag="ACHJWB_000231"
     CDS             29866..31170
                     /gene="pbp4b"
                     /locus_tag="ACHJWB_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4311926.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGVNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(31228..32127)
                     /gene="yfeX"
                     /locus_tag="ACHJWB_000232"
     CDS             complement(31228..32127)
                     /gene="yfeX"
                     /locus_tag="ACHJWB_000232"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4311927.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(32223..32798)
                     /gene="yfeY"
                     /locus_tag="ACHJWB_000233"
     CDS             complement(32223..32798)
                     /gene="yfeY"
                     /locus_tag="ACHJWB_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4311928.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSKQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(32859..33308)
                     /gene="yfeZ"
                     /locus_tag="ACHJWB_000234"
     CDS             complement(32859..33308)
                     /gene="yfeZ"
                     /locus_tag="ACHJWB_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4311929.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWGLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(33295..33720)
                     /gene="ypeA"
                     /locus_tag="ACHJWB_000235"
     CDS             complement(33295..33720)
                     /gene="ypeA"
                     /locus_tag="ACHJWB_000235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4311930.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            33934..34803
                     /gene="amiA"
                     /locus_tag="ACHJWB_000236"
     CDS             33934..34803
                     /gene="amiA"
                     /locus_tag="ACHJWB_000236"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4311931.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTEAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            34807..35706
                     /gene="hemF"
                     /locus_tag="ACHJWB_000237"
     CDS             34807..35706
                     /gene="hemF"
                     /locus_tag="ACHJWB_000237"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4311932.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(35712..36764)
                     /gene="eutR"
                     /locus_tag="ACHJWB_000238"
     CDS             complement(35712..36764)
                     /gene="eutR"
                     /locus_tag="ACHJWB_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4311933.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(36810..37310)
                     /gene="eutK"
                     /locus_tag="ACHJWB_000239"
     CDS             complement(36810..37310)
                     /gene="eutK"
                     /locus_tag="ACHJWB_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4311934.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(37323..37982)
                     /gene="eutL"
                     /locus_tag="ACHJWB_000240"
     CDS             complement(37323..37982)
                     /gene="eutL"
                     /locus_tag="ACHJWB_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4311935.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(37992..38879)
                     /gene="eutC"
                     /locus_tag="ACHJWB_000241"
     CDS             complement(37992..38879)
                     /gene="eutC"
                     /locus_tag="ACHJWB_000241"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4311936.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLAELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(38900..40261)
                     /gene="eutB"
                     /locus_tag="ACHJWB_000242"
     CDS             complement(38900..40261)
                     /gene="eutB"
                     /locus_tag="ACHJWB_000242"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4311937.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(40273..41676)
                     /gene="eutA"
                     /locus_tag="ACHJWB_000243"
     CDS             complement(40273..41676)
                     /gene="eutA"
                     /locus_tag="ACHJWB_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4311938.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGVQTLSEQRLCRVLNIDIGGGTANY
                     ALFDVGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECLGAGTDVRSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(41673..42899)
                     /gene="eutH"
                     /locus_tag="ACHJWB_000244"
     CDS             complement(41673..42899)
                     /gene="eutH"
                     /locus_tag="ACHJWB_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4311939.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(43015..44202)
                     /gene="eutG"
                     /locus_tag="ACHJWB_000245"
     CDS             complement(43015..44202)
                     /gene="eutG"
                     /locus_tag="ACHJWB_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4311940.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMVHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(44222..45028)
                     /gene="eutJ"
                     /locus_tag="ACHJWB_000246"
     CDS             complement(44222..45028)
                     /gene="eutJ"
                     /locus_tag="ACHJWB_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4311941.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSG"
     gene            complement(45039..46442)
                     /gene="eutE"
                     /locus_tag="ACHJWB_000247"
     CDS             complement(45039..46442)
                     /gene="eutE"
                     /locus_tag="ACHJWB_000247"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO4311942.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(46454..46741)
                     /gene="eutN"
                     /locus_tag="ACHJWB_000248"
     CDS             complement(46454..46741)
                     /gene="eutN"
                     /locus_tag="ACHJWB_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO4311943.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(46848..47141)
                     /gene="eutM"
                     /locus_tag="ACHJWB_000249"
     CDS             complement(46848..47141)
                     /gene="eutM"
                     /locus_tag="ACHJWB_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4311944.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(47180..48196)
                     /gene="pta"
                     /locus_tag="ACHJWB_000250"
     CDS             complement(47180..48196)
                     /gene="pta"
                     /locus_tag="ACHJWB_000250"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4311945.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQCLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(48193..48996)
                     /gene="eutT"
                     /locus_tag="ACHJWB_000251"
     CDS             complement(48193..48996)
                     /gene="eutT"
                     /locus_tag="ACHJWB_000251"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4311946.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITHSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(48993..49694)
                     /gene="eutQ"
                     /locus_tag="ACHJWB_000252"
     CDS             complement(48993..49694)
                     /gene="eutQ"
                     /locus_tag="ACHJWB_000252"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4311947.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(49669..50148)
                     /gene="eutP"
                     /locus_tag="ACHJWB_000253"
     CDS             complement(49669..50148)
                     /gene="eutP"
                     /locus_tag="ACHJWB_000253"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4311948.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(50161..50496)
                     /gene="eutS"
                     /locus_tag="ACHJWB_000254"
     CDS             complement(50161..50496)
                     /gene="eutS"
                     /locus_tag="ACHJWB_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4311949.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(50789..53068)
                     /gene="maeB"
                     /locus_tag="ACHJWB_000255"
     CDS             complement(50789..53068)
                     /gene="maeB"
                     /locus_tag="ACHJWB_000255"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4311950.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            53357..54307
                     /gene="tal"
                     /locus_tag="ACHJWB_000256"
     CDS             53357..54307
                     /gene="tal"
                     /locus_tag="ACHJWB_000256"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4311951.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            54327..56330
                     /gene="tkt"
                     /locus_tag="ACHJWB_000257"
     CDS             54327..56330
                     /gene="tkt"
                     /locus_tag="ACHJWB_000257"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4311952.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNDISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(56426..57469)
                     /gene="ypfG"
                     /locus_tag="ACHJWB_000258"
     CDS             complement(56426..57469)
                     /gene="ypfG"
                     /locus_tag="ACHJWB_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4311953.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(57595..58170)
                     /gene="nudK"
                     /locus_tag="ACHJWB_000259"
     CDS             complement(57595..58170)
                     /gene="nudK"
                     /locus_tag="ACHJWB_000259"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4311954.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(58238..60217)
                     /gene="aegA"
                     /locus_tag="ACHJWB_000260"
     CDS             complement(58238..60217)
                     /gene="aegA"
                     /locus_tag="ACHJWB_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4311955.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            60423..62123
                     /gene="narQ"
                     /locus_tag="ACHJWB_000261"
     CDS             60423..62123
                     /gene="narQ"
                     /locus_tag="ACHJWB_000261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4311956.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            62287..65400
                     /gene="acrD"
                     /locus_tag="ACHJWB_000262"
     CDS             62287..65400
                     /gene="acrD"
                     /locus_tag="ACHJWB_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4311957.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(65499..65558)
                     /gene="ypfM"
                     /locus_tag="ACHJWB_000263"
     CDS             complement(65499..65558)
                     /gene="ypfM"
                     /locus_tag="ACHJWB_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4311958.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        15508 a        18047 c        16714 g        15449 t
ORIGIN      
        1 ccgttgagga cagtccggca gcaaccagac ttaacccaaa gaccgttgcc gcagcatggc
       61 ttaacagcgg ttgcagcgtc agataagcct catcaagatc ggcaacacca gtatgaccag
      121 aaaagtggaa cgccgccgca gctgtagcca tcatcgccag attgacaaaa ccggcaatcg
      181 tcatggcgat agccacatcc catttggtgg cggaataacg ttgttgacgc gaaccgccat
      241 gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg cggcataatc gtcgccccta
      301 acacgcctgc tgccaggaag accgcctccg aagtaggtaa actcgggatc accattcctt
      361 tacccagctg cgccaggtta ggctgggaga aaatcaactc gacaatgtaa gccgcggcaa
      421 caaacaacag taacccgcca atcactttct ccagcggttt ttgcccgcga cgttgcagca
      481 ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc ctgcaacaac gaaacaccaa
      541 gaatgagttt aaaaccgatc gccgcaccaa taaattccgc caggtcggtt gccatcgcaa
      601 taatttctgc ctgaacccaa tagaaccaca ctacgggacg cggatagtga tcacgaatct
      661 gctccgccag atttttaccg gtggcaatcc ctagtttggc agagaggagc tgaatcagca
      721 tcgccatcag gttggcccaa acgacaaccc acagtagctg atagccgaag ctagcacccg
      781 cctgaatatt ggtcgcaaag ttaccgggat cgatataacc aatcgccgca atgaacgcag
      841 gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc gctgctactc tcaacgcgat
      901 agttcgtcat cttgtgcctc taaaacatag cctttgctat gtttcatgct atgccaaacg
      961 agaatgatta tcaaattcat ttgaatgggt tgtggtgatt tctttgatag accaggatta
     1021 tgactacgaa aagattgatg acttgaatgt tatggttatg tttaatgtta gcacatttac
     1081 ataactttca gcttccatac acaacatagc agaaatgtat gacagatcac tatttttgaa
     1141 gcttgtcaca ggacgtcatt atagtgtgtg tcagatctcg ttttccttaa ccatgttaca
     1201 tagaatgtgc acggaaattt aacctgcctc atatttggag caaatatgga ccgcgtcctt
     1261 cattttgtac tggcacttgc cgttgttgcg attctcgcac tgctggtaag cagcgaccgc
     1321 aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta tcgaagtgtt actggcgtgg
     1381 ttcttcctga actccgacgt tggtttaggc ttcgtgaaag gcttctccga aatgttcgaa
     1441 aaactgctcg gatttgccaa cgaagggact aacttcgtct ttggtagcat gaatgatcaa
     1501 ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg tctttatctc tgcactgatc
     1561 ggtattctcc agcacattcg cgtgttgccg gttattatcc gcgcaattgg tttcctgctc
     1621 tccaaagtca acggtatggg caaactggaa tcctttaacg ccgtcagctc cctgattctg
     1681 ggtcagtctg aaaactttat tgcctataaa gatatcctcg gcaaaatctc ccgtaatcgt
     1741 atgtacacca tggctgccac ggcaatgtcc accgtgtcga tgtccatcgt tggtgcatac
     1801 atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg tactgaacat gttcagcacc
     1861 tttatcgtgc tgtcgctgat caatccttac cgtgttgatg ccagtgaaga aaacatccag
     1921 atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc tgggtgaata cattctggca
     1981 ggtttcaaag ttgccattat cgttgccgcg atgctgatcg gctttatcgc cctgatcgcc
     2041 gcgctgaacg cactgtttgc caccgttact ggctggtttg gctacagcat ctccttccag
     2101 ggcatcctgg gctacatctt ctatccgatt gcatgggtga tgggtgttcc ttccagtgaa
     2161 gcactgcaag tgggcagtat catggcgacc aaactggttt ccaacgagtt cgttgcgatg
     2221 atggatctgc agaaaattgc ttccacgctc tctccgcgtg ctgaaggcat catctctgtg
     2281 ttcctggttt ccttcgctaa cttctcttca atcgggatta tcgcaggtgc agttaaaggc
     2341 ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc tgaaactggt ttacggctct
     2401 accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg tgctgtaaaa ccatacataa
     2461 aaaagccggg gatcattccc cggctttttt acgcctgtta atcagctaat ggctgcgggc
     2521 gaccaatcaa atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct
     2581 gttgcgtctc gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa
     2641 ttgatctcac aatcatcgca tccagcgtat tcgtgacaat atctttcaca aagacgccat
     2701 caattttgat gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tatccggtgc
     2761 caaagtcatc aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc
     2821 tgctttctgc gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg
     2881 agatgtgata acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg
     2941 tgagcggcat taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca
     3001 gccacttcaa caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag
     3061 caataagggg cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga
     3121 ggatctcatc ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat
     3181 caccctgatc taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc
     3241 cggaaaccat ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct
     3301 ccgccagcca gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac
     3361 gcccccaggc agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac
     3421 tattcaggat gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta
     3481 acagcagttc actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca
     3541 gcgtacggca aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca
     3601 taaactcaag attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg
     3661 cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg atctgtcagc gcctgcaaat
     3721 gccagcggcg attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa
     3781 cgctaaagga aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt
     3841 gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta
     3901 aaaacggata gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca
     3961 agtaaccggc aataaagtcg ttttcataag gtgtgcacaa cagaagtagc agcacgctta
     4021 acgctgccag ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggagcgatat
     4081 ccctgcgcca caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga
     4141 aaagcatgtt gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa
     4201 tcgcatccgc atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca
     4261 caagatacat gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt
     4321 gccagacata tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata
     4381 ttgcgcaggc acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa
     4441 aagagagatg aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac
     4501 cgcgccagcc aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa
     4561 agatataact ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta
     4621 ccacggtgag cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca
     4681 caaacatgag tacactactc tcactgatcg gggttttcag gcacacaaat aataagttta
     4741 ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat ttttcatcca atgatgcttt
     4801 caccaggcgg cgctaacccg gacgtagact gtattacaaa agcggcaaaa agcagagaca
     4861 aaaaaccccc gctttgcagc gagggttgga aatttggtgg agctaagcgg gatcgaaccg
     4921 ctgacctctt gcatgccatg caagcgctct cccagctgag ctatagcccc acgatgcgtt
     4981 tacgtaccaa atttgctggg tgcaaaattt ggtggagcta agcgggatcg aaccgctgac
     5041 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccgtcac gtaaagcttg
     5101 tcgagttgac gggcggcatc atatgaattc cgcccgaatg tgtcaacggc aaattgcaac
     5161 gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat tttggaaaac cgctcgcatt
     5221 cagtagttat tcatgtcacg gtttcctgta aagtggtgtt ataaaatgaa ctactaatag
     5281 acccacatac attcagggaa ttgttatgtt caaggagagg atgacaccag atgaacttgc
     5341 cagactgacc ggctatagcc gccagaccat taataaatgg gtacgcaagg aaggctggac
     5401 aacgtcacca aaacctggcg tccagggtgg caaagcccga ctggttcacg tcaatgaaca
     5461 ggttcgtgaa tatattcgca atgctgaacg tccagaaggt cagggagaag cgcctgccct
     5521 ttctggtgat gcgccgcttg aagtattgct ggtgacactg gcgaaagaga tgacgccagt
     5581 tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg attatcggat tgttacaacg
     5641 cttagggatt cgcgatagca aataatatga aaagattacg caacaaaatg accaccgaag
     5701 aactggctga atgcctcggt gtggctaaac aaactgttaa ccgttggatc agagaaaaag
     5761 gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg tgccagactt attctggtcg
     5821 atacgcaagt ttgcgagttt attcagaaca cgccagcctt ccataacaca ccaatgttga
     5881 tggaagctga agagagaatt gctgaatacg ctcctggagc acgcgccccg gcgtatcgac
     5941 aaatcatcaa cgcgatagat aatatgacag acattgagca ggaaaaagtc gcgcaatttt
     6001 tgtcacgtga aggaattcgc aatttcctcg cccgtctcga tattgacgaa tcagcataaa
     6061 taaaaaacgg caggatatta tctcctgccg tttatctttt tacacgctaa ttactgctga
     6121 ttttcgcgtt cagcaataaa atccagcgct ttgttgatac gctcgatact gcgggtctta
     6181 ccaattgcgt gaacggtaac atccagtgct ggagactgcc ccgcaccggt tacggcgaca
     6241 cgcagcggca taccaacttt acccataccc acttccagct catccgccgt cgcctgaata
     6301 gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact
     6361 tccagcggct gacgcgctac cggacgcaga tgttttttcg ccgcatcggc atcgaactca
     6421 gcaaaatctt cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt
     6481 tcgcccagca gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc
     6541 tgctcaatgt gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag
     6601 tgatggttca gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca
     6661 ttcaaagtga agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac
     6721 cagcccagac gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac
     6781 tgcattacgc tgactgcccc gtgacgtttg gacagttttt taccgtcatc gccattgatc
     6841 atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg
     6901 cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag
     6961 tcatcgacaa ccacacagaa gttataggtt ggggaaccat cggtacggcg gataataaga
     7021 tcgtccagtt cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca
     7081 gaaccttcct gcgggttagc aaaacgtaca acacacggtt catcatcagc atgatgctca
     7141 tggctgtggc ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg
     7201 cgcagcgctt ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc
     7261 atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca
     7321 tcccactcca ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg
     7381 gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca
     7441 aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt
     7501 ggcgcgaagc gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac
     7561 cggcaaatcc tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat
     7621 gtagttcggg tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc
     7681 tgttttgcga tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt
     7741 tgactcattt tgaactctcc ctataatgcg actccacaca gcgggggtga ttagctcagc
     7801 tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca
     7861 actactttat gtagtctccg ccgtgtagca agaaattgag aagtgggtga ttagctcagc
     7921 tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca
     7981 ctttctcgcc agctaaattt cttgtaaaaa tgtgaagtac cgaagtgggt gattagctca
     8041 gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac
     8101 cacttcgggt cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt
     8161 tcgaatcctg cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctatct
     8221 gcgatactca aagattcgaa cctgcagcag gtttgagttg agcgcagcga aacaaagccg
     8281 ctcgcggcga cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgta
     8341 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct
     8401 caagttcctt gttcttctgc cgatattacc tcgtacagtg acgaggactg aacaatggcc
     8461 actatctctt caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa
     8521 ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa
     8581 caaattaaag agattgtaga cactagcggc agtgctgaag acaaacaaaa acaggccgaa
     8641 ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca gagacagcag
     8701 gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct
     8761 gttgaaaata cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca
     8821 gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa
     8881 agtgaacggt tcttcctgcg caccagcatc aactggcgtt caacaaccgg cgtaatgcgc
     8941 tttaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg
     9001 ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata
     9061 ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg
     9121 taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg
     9181 gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat
     9241 tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga
     9301 atactttcca tcacccactg ctgcggacga tcgtgcagga caaactgaat atctggatag
     9361 cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca
     9421 gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg
     9481 ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc
     9541 accacttcac gcgtggttct atccagcagg cgaacaccag tatgattttc cagctccttc
     9601 acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt
     9661 ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt
     9721 cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa
     9781 ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac
     9841 cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt
     9901 ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt
     9961 gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg
    10021 cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa
    10081 tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca
    10141 gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc
    10201 gtcggcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa
    10261 tgttcacggt gcagggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct
    10321 gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa
    10381 aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag
    10441 cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt
    10501 ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg
    10561 actgggcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct
    10621 ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt
    10681 gctgccccta atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg
    10741 atacaaacgc cagaccgaac agttacaggc acagcaggaa agcagcgccg ataaagctta
    10801 aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt
    10861 gatttgcatt agctcaccga gcttccctgc cgggaattca tctttgcggg caaaccacag
    10921 cagatactcc tccggcaggt cgattaagcg acgcccttta tatttgccaa acggcattat
    10981 cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca
    11041 tttccgcttc gtcgatgact tcaatgccca gttcctgcgc tttcgccagt ttagaacctg
    11101 ccgcttctcc ggcaatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc
    11161 ccagttcaac cagtcgagct ttagcgtcat cacgcgacat ctggcttaat ctgcccgtaa
    11221 gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg
    11281 caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg
    11341 caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct
    11401 cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg
    11461 cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt
    11521 ctttcgcttt ttctagcgcg ttaaccacgt tttgtgccga ttttggcccc atacgctcca
    11581 gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt
    11641 tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg
    11701 aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc
    11761 gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg
    11821 ggaatacaac ctcacgggta tcttccgggc gttcagaaag tacgacgtta accacctgcg
    11881 ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt
    11941 cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag
    12001 gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac
    12061 gcacaaaggt catctgctcc tgcgccggga atttaaacgc taccgcccag cgcggggcac
    12121 gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt
    12181 cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt
    12241 cttccgccga ttcacaaagc gttacccgat cgctgaccgg caacccccac tttttaaatt
    12301 gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat
    12361 agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac
    12421 cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt
    12481 taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg
    12541 ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga
    12601 tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat
    12661 acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt
    12721 tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa
    12781 cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg
    12841 cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca
    12901 gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca
    12961 tcacatgata aagatattca tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt
    13021 cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga
    13081 ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc
    13141 tggtactcgc gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg
    13201 cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg
    13261 tcaccgtaag acggtacctg cataaagatg gtgacacccg gagtagtgaa atccttcatt
    13321 tcaggatcaa agattcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg
    13381 ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt
    13441 tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc
    13501 atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca
    13561 ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc
    13621 ggtgctgaat gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac
    13681 ggctgttcag gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca
    13741 tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac
    13801 gcataaggcg gttggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg
    13861 ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg
    13921 acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg
    13981 cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt
    14041 atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct
    14101 gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta
    14161 cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt
    14221 tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag
    14281 aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct
    14341 gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg
    14401 attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc
    14461 tggtggctct ttacacagct cgatgtctgg atcccgactc tcatgagtta cgttccggac
    14521 tggctacaat ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg
    14581 tttggctatt tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg
    14641 gctgaacaac tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt
    14701 atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctt
    14761 ccgcgcgcaa ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg
    14821 ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc
    14881 ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc
    14941 accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg
    15001 ttcatcatgc ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat
    15061 aaacacgcga tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca
    15121 ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata
    15181 gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc
    15241 aatttcatac agttaaggac aggccatgag taagattttt gaagataact cgctgactat
    15301 cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt
    15361 ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga
    15421 tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccaa ccagcggtaa
    15481 taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat
    15541 gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt
    15601 gctgacggaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc
    15661 cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat
    15721 tcacgaaaaa accaccggtc cggagatctg ggaagatacc gacggtcagg ttgatgtatt
    15781 tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac
    15841 caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc
    15901 ccaggcactg gctggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc
    15961 tggttttatc ccagctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa
    16021 tgaagaagcg atttctaccg cgcgtcgtct gatggaagaa gaaggtattc ttgcaggtat
    16081 ctcttctgga gcagctgttg ctgcggcgtt gaaactacaa gaagatgaaa gctttaccaa
    16141 caagaatatt gtggttattc taccgtcatc gggtgagcgt tatttaagca ccgcattgtt
    16201 tgccgatctc ttcactgaga aagaactgca acagtaatgc cagcttgtta aaaatgcgta
    16261 aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca
    16321 ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta
    16381 atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta
    16441 gcggcataat gtttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact
    16501 gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag
    16561 tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg gggaaataca
    16621 atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc
    16681 cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa
    16741 agcgccagcg cgaaaagctt gtttaaactg cagactctgg gcctgactca aggtaccgtt
    16801 gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg
    16861 atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg
    16921 ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga
    16981 aagaagacga aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag
    17041 ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga
    17101 aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc
    17161 tcgaagatga ggagctggag caggaaatca tagccctgat taaagataag cacatgacag
    17221 ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg
    17281 atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca
    17341 acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg
    17401 ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca
    17461 tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc
    17521 ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc
    17581 tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc
    17641 gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc
    17701 cagctattac gctggacggt caccaggtag aagtgtgcgc taacattggt acggttcgtg
    17761 acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc
    17821 tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag
    17881 tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca
    17941 aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta
    18001 tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg
    18061 cctcggcttt cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc
    18121 gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt
    18181 ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac
    18241 gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca
    18301 ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccaacca atgtcaccgt
    18361 ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg
    18421 gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc
    18481 tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca
    18541 cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg
    18601 agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat
    18661 gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg
    18721 gtttccgacg acaagaagga taccggaact attgagatca ttgctccgct ctctggcgag
    18781 atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt
    18841 attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa
    18901 atctttgaaa ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc
    18961 cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa
    19021 ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg atctgccgct actggaagag
    19081 aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg
    19141 atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa
    19201 ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc
    19261 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    19321 aaccccgcac ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc
    19381 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg
    19441 acattcacgc tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg
    19501 gaggcgctgg taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca
    19561 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg
    19621 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt
    19681 ttgacataaa ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca
    19741 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    19801 cccatatagc ccgtggttac agcgcgcagc tggcgcagcg catcacgctc ctgaagcgca
    19861 cgcaaataac cgctaaacca ttcatccgga atcgcaccac cgtagaaagt gtcataatgc
    19921 ggcgtattgc tcagcaatac cgtcggcacg gcaaaaacat tcagaccgtt ctgtttgata
    19981 gcaggcacgg caatgctgtt gcccacgctg ccgtaaacca cctgcgactg cacggcgacg
    20041 atatccgcct gcagcgccct gctcttatcg ttaaacaaca acaaactact catttaattt
    20101 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    20161 caaatcggct atcacaaaac aaggataagg taattcaatg aagaaaatca tttgtctggt
    20221 cattacacta ctaatgacac tccccgctta cgcgaagtta actgcccatg aagaagcccg
    20281 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    20341 tgatgaacat acctgcgatg aagcggtttc tcatctgcgt ctgaagctgg gcaatacccg
    20401 taaccgcatt gacactgccg agcagtttat tgataaggtt gcttcgtcgt catcgattac
    20461 cgggaagccg tatattgtga agatccccgg taagagcgat gagaacgcac agcctttttt
    20521 acatgcgcta attgcgcaga cggataaaac ggtgcctgcg caataatctt aaatccccgc
    20581 cccctggcta aaatgctctt ccccaaacac cccggtggaa aggtagcgat cgccacgatc
    20641 gcagatgatc gctaccacca ccgcaccagg gttagctttt gccacccgca gtgctccggc
    20701 aaccgcgccg ccggagctga cgccacagaa tattccttcc cgcactgcca gttcgcgcat
    20761 ggtgttttct gcatcgcgct gatgaatatc cagcacctca tccaccagag aagcgttgaa
    20821 aatccccggc agatattccg caggccagcg gcgaatgccg ggaatactgc tgccttcttc
    20881 cggctgcagg ccaacaatcg ttactggttt ggattgttcg cgcataaagc gtgaaacgcc
    20941 ggtgatagtg ccggttgttc ccatgctgga gacaaaatgg gtaatgcgcc cgccggtttg
    21001 ctgccagatt tccggcccgg tagtggtgta atgggcgtaa gggttatcgg gattattgaa
    21061 ctgatcgagc agctttcctt cgccacgatt cgccatctcc agcgccagat cgcgcgcacc
    21121 ttccatgccc tgctctttgg tgacaagaat cagttccgca ccataagcac gcatcgccgc
    21181 acggcgttcc tggctcatgt tgtcgggcat cagcaatttc atgcgatagc ctttcagcgc
    21241 ggcaatcatt gccagcgcaa tgccggtgtt accactggtg gcttcgatta agacatcacc
    21301 cggtttaatt tccccgcgct tttccgcctc gacgatcatc gaaagtgccg cacgatcttt
    21361 caccgaacct gccgggttat tgccttccag ttttaaccac acttcactgc cgttatccgg
    21421 ccccattcgc tgcaacttca ccagaggcgt attgcctatt gtttgttcta atgtactcac
    21481 gatctctatc catacgtggt gttgcctgat gcgacgcttg cgcgtcttat caggtctaca
    21541 ggttacaaac cttgccatta aaaagcccgg atcgcgggaa gcgcctccgg gcgtttaaca
    21601 ttcactcaac ctatcaggcg ctttgtgcga gagcaagttc ctcatcgcgg gtttcgatac
    21661 gctcgtcgcc gttatacagc cgcgcatgtt gcagaccaac gaataaacgc tcgccacgct
    21721 gcggggcatc gtcgccatgc atcacgaccg tcagcggttc gttgtaccac cccagcggct
    21781 gcaccactaa ttgggtgtag tgacctttcg ggctggcttc cagtacctgt accggcagcg
    21841 gcgaatcgag actggtacgg cggctgatat ccacttccca cgggcgcagg aagagatcca
    21901 ccggcccctg ataagcaggt gtgtagccca gcggccagcg atgtgcgcca acatggaact
    21961 gcccgccgcg aatggttccc tgcaggcggt tcacttcgcc cataaattcg agcacaaaac
    22021 gggtcgctgg ttcgcgccat acctgctccg gcgcatcagc ctgttcaata ttgccctggc
    22081 tcatcaccac tacacgatca gctacttccg tcgcttcttc ctggtcgtgg gtcacgaaca
    22141 cgctggtgaa tttcagctct tcatgcagtt gacgcagcca gcgacgcagc tctttacgca
    22201 cctgcgcatc cagcgcgcca aacggttcat caagcagcag aatttgcggt tctacagcca
    22261 gcgcgcgcgc cagcgccacg cgctgtttct ggccgccgga aagctgtgcc ggataacgat
    22321 ctgccagatg agcaagctgc accatctcca gcaattttgt cactttcgct ttgatggctg
    22381 cggcattcgg gcgctcgcga cgtggcagca ccgtcaggcc aaaagcgata ttgtcgaaca
    22441 ccgtcatatg acggaacagc gcgtaatgct ggaacacgaa accgacttta cgatcgcgtg
    22501 cgtgcaggcg gctcacgtcg gtaccgtgga agcgaatatg cccgctggtt tgatgctcca
    22561 gcccggcaat aatgcgcagc agcgtggttt ttccggaacc ggacggcccc agcagcgcga
    22621 ccatctgacc agaaggaata tccagtgaga tatcgttcag cacctgggtg cgaccaaacg
    22681 acttcttaat attggcaatc tcaatgctca tgatgctcct cctgctgcgc gcgtttttcc
    22741 tgattctcca gacgccactg caacatactc tttaaaaaca gggtgataat cgccatcagc
    22801 gtcaacagcg cggcggcggt aaaggagccg acggtgttgt agtcctgctc cagcaattca
    22861 atctgtaacg gcagcgaaag tgtttcgccg cgaatcgagc cggaaaccac cgacaccgca
    22921 ccaaactcgc caatcgcgcg ggcgttggtt agcaccacac catacagcag cgcccagcga
    22981 atgttcggca atgtgacgcg acggaacatc tgccagccag acgcgccaag caaaatcgcc
    23041 gcttcgtctt cctggctgcc ctggcttaac atcaccggca ccagctcgcg caccacaaaa
    23101 ggacaggtca caaagatggt gaccagcacc attcccggcc aggagaacat aatctgcaaa
    23161 ttatgttcat ccagccagcc gcccagcgga ccgttagagc cgtagaacag cagatacacc
    23221 agaccggcaa ccaccggcga tacggcaaac ggaatgtcca gtagcgtcag cagtaactgg
    23281 cgtccgggga agttaaagcg cgttaccagc cacgccagca gaatgccgaa cactaggttc
    23341 accggtacgg caatcagcgc aatcatcacc gtcagccaaa tggcgtgcag catgtccgga
    23401 tcggccagat tctgtaaaac cggcatcagc cctttgctga acgcctgtac gaagatataa
    23461 atcatcggca ccagcagaat gaacgccgat accagcatcc cgatgccaat cagaaaccat
    23521 ttgccccagt taatcgggcg cgcgtcataa cgcttcaatt gggtaacttc cgccattaat
    23581 gacctaccac acgccgacca aagcgacttt gcagagtgtt aattgagaac agcagcagca
    23641 gggatgccgc gaggatcacc gaagcaatcg cgctcgccgc cgggtaatca aactcctgca
    23701 agcgcacaaa aatcatcagc gacgtcactt ccgtcttcca ggcgatgttc ccggcgataa
    23761 atatcaccgc gccaaactca ccaagactac gggtaaacga caacgccacg cccgccacca
    23821 gcgctggaga aagttccggc agcaccactt tgcagaaact ctgccagcgc gttgcaccaa
    23881 gcgtttccgc cgcttcttca tattctgggc ctaactcttc cagcaccggc tgcacggtac
    23941 gcaccacaaa cggaatgctg gtaaaggcca tcgccaccgc aataccaagc caggtatagg
    24001 tgactttgat atcaaacttc gccagccatt caccgtaaaa accgtttacg gaaaagagcg
    24061 aggccagcgt taaaccggcg actgccgttg gcagcgcaaa gggtaaatcc atcagcgcat
    24121 caagcagcgt gcggcccgga aagcgataac gtgttaaaat ccacgccatc agcagaccga
    24181 aaacgccgtt aaaaatcgat gccacaaacg ccgacagcag cgtcactttg taggccgcga
    24241 ccacttgtgg gttggtgatc acctcccagt actgcgccca gctcatctga gccagctgca
    24301 tcaccagcgc ggagagcggc agcagcaaaa tcaggcacac aaacagcaga ctggtgccga
    24361 ggcttaaggt aaagcccggc agcacgcgtc ttgatgaaac tgcaaacatc agttacgccc
    24421 cgccgctaac agcttgtcta actcgccgcc gctggtgaag tgggttttca tcacttccgg
    24481 ccaggagcca aatttgtctt ccacgcggaa cagctcggtc tgcgggaatt tatctttcag
    24541 tttgtccatg acttccgggt tatttacgcg gtagtaatag tcggtgatga tggtctgcgc
    24601 ctgcgggctg tagagccagt tcaggtaggc ttttgctgct ttttccgtac cgttggcctg
    24661 cacgttttta tcgacccacg ccaccgggaa ttccgccaga atgttggttt tcggaatcac
    24721 cacttcaaag ccttgcgctt catactgttt acggatgttg ttcacttccg actcgaagct
    24781 gatcagtaca tcgcccaggc cgcgctcggc aaaagtggtg gtcgcgccac gaccgccagt
    24841 atcgaacact tcaacgtttt tcaggaactg ggtcataaac tgttcggttt tggctttgtc
    24901 gccaccatca gctttatccg ctgcgcccca tgccgccaga taggtataac gcgcgttacc
    24961 cgacgttttc gggttcggga aaatcagctt cacgtcggag cgcaccaggt cgttccagtc
    25021 gtggatattc ttcgggttgc ccttacgcac caggaagccc atggtggagt agaacggcga
    25081 gctattattc ggcaggcgcg actgccagtc agccgggatc agcttgcctt tgtcgtgcag
    25141 aatttgtacg tcggtcacct ggttataagt gacaacgtcg gcttttaagc cctgcaaaat
    25201 tgccagcgcc tgttttgatg acccggcatg agattgtttt atcgtcagtt tgtcgccgcc
    25261 gttatctttg gcccattgct gttcaaacgg cgggttcagg gcggcaaaca gctcgcggga
    25321 gacgtcataa gaactgttca gcagttccgt tgcctgtaca tggcccgcca gcagcagaga
    25381 agcgaccagc gcgagtgagt tctttttcag taagttaacg gccattgcgc acccttataa
    25441 atttaatgac tttctaatag ccatcatatt tataacggac gtgaaaggag taacggtttt
    25501 atataccgtt tggtgatttg gaagttgaaa agggaataag aaccgggttt aaaacgaaat
    25561 cctccccaca aatgcaggga ggaaacagag gtgaatcaga taccgacgct aaccgtctcc
    25621 ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg ttaaatagct ggattcatcc
    25681 gatgcgagga aggccgccag ttcgccgact tccagcggat cggcgaggcg acgcatcggg
    25741 attgctttcg ccatttcagt cagcaccgac tctggatctt ccgggttcga ctggcgggca
    25801 atgctttccg ccattggtgt gcgcacgtat cccgggcaaa tggcgttaac gcgaatacca
    25861 gactgcgcgt actccaccgc cagcgatttt gtcaggccaa caatcgccgc tttcgttaag
    25921 gcgtacgccg tttcgccagg atcggccacc atatcaccag tgactgaaga catcatcaca
    25981 atgcgaccat ctttgcgggc aatcatctcc ggcagcaccg ccttcgtgac gttccatacg
    26041 cctttaatat tgatgtcaat atggaaatcg cgatcgtcat cgctcatatc gaggaaactg
    26101 cccagacgac aaacgcctgc gttattcacc aggatatcaa tgcgcccttc tttttccttc
    26161 gcgcgtttga tagctgcggc taccgacgcc gggtcacgca catcggcgac aaccgccgta
    26221 cagcgatgac cacgaccaca cagttcgtcc gccagctttt cgatctcagg ggagatatcc
    26281 agcaagatta ggtttgcgcc atgacgcgca aaagttctgg caattccttc gccaattccc
    26341 tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt tacccatttt aatgctctcc
    26401 ttgttaatac agagcgttac actccattcc ctctgaaaat acaggataaa caaaatattg
    26461 ctgcggatca gctcactaat tcaggaaaat ctcaatccag tcgctgcttt atgcatcatt
    26521 tcaggcgttg tgttagctca ctgctgcgct gaatcatttt caatgactcc acatcattga
    26581 gttgcacaag accaacaaac agtaaatcgg taacagaatt ttgcgccgtg cgggtagaca
    26641 tcgacgagct acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa tgcgccagcc
    26701 gccgcagcgg agagtcagcc agagaagtaa tggcaatcac cgttgccccc tgctttcgtg
    26761 ccgcttctgc gcacaagaca atctcttttt tactgccgct gtaagagata gcgatttgta
    26821 catcgccttt tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtca gcttcacagg
    26881 ccacgcgata gccaattttc atcagtttga acgacagatc gcgccccacc agcgccgacc
    26941 cgcccaggcc cgttatctgg ataaacggtg ctttactgat gacctcaatg attttttgca
    27001 gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc cagctctttt tcgcgattca
    27061 gtttgcgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc aggtgcagcg
    27121 ccgtggcatt cgttttctcc cggctggcac tgtattcgcc aattaacgcc atacgcaatt
    27181 cagtaaatcc ttgcgcgccg agcttctggg caaatttcac aatgctcgac tgactgatgc
    27241 cgagctgttt cgccatcttg cgcgaagaga cggatttcag ctcactcacg ttggcccgca
    27301 gaaagtcagc aattttttgc tcgttctcgg taaattcact ttccgcattg cgtattttcg
    27361 tcaggtacaa catcttccac tctcccgggc tggcagcaaa aattccgctt aagttcttat
    27421 gacgctcttc acactctgcg agtgtagact caatgattcc tttaagcctg tcaattcggg
    27481 atatatgatt ccatatatct gcaattattt gatcgtaaat agtaaggtca ccaccgatgc
    27541 aacttgaaaa gatgattact gaaggctcga acgccgcctc ggctgaaatt gaccgcgtat
    27601 caacgctgga aatgtgctgg attatcaacg atgaagataa aaccgtaccg cttgccgttg
    27661 agcgcgtact gccggatatc gccgcgtcga tcgatgttat ccacgcccag gtaagcggcg
    27721 gcgggcgtct gatttacctc ggtgcgggaa catccggtcg tctggggatt ctggatgcca
    27781 gcgaatgtcc gcccacctac ggcgtgaaac cgggtctggt ggttggtttg attgctggcg
    27841 gcgaatatgc cattcagcac gcggtggaag gcgcggaaga tagccgggaa ggcggtgtta
    27901 atgatttgaa aaacatcggc ttaaccgcgc aggatgtggt ggtcggtatt gccgccagcg
    27961 gtcgtacgcc gtatgtgatt gccggactgg aatacgcccg ccagctcggt tgccgcacag
    28021 tgggaatttc ctgtaatccg gggagtgccg tttccaccac cgccgagttt gccattacac
    28081 cgattgtagg tgctgaagtg gtcactggtt cttcgcgaat gaaagcaggc acagcgcaga
    28141 aactggtgct caatatgctt tccaccgggc tgatgattaa atccggcaaa gtgttcggca
    28201 acctgatggt cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag gtcaatatcg
    28261 ttaaaaacgc caccggatgt aatgccgaac aagcggaagc ggcgttaatt gcctgcgagc
    28321 gcaactgtaa aacagccatt gtgatggtgc tgaaaaatct cgatgccgca gaagctaaaa
    28381 aacgcctgga tcaacacggc ggctttattc gtcaggtttt agacaaggaa taacccatgg
    28441 ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc ggaccgggaa
    28501 atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat gacagctcac
    28561 tggttgatcc caatatcaaa acgctggaag gcgtgaaggg cgtcattttg accagcgacc
    28621 aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg atgagcgagc
    28681 tgctgggaga agcaccggta caggatgccg cagaaatcgc cgcccagaac aaacgtcagt
    28741 taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc accatcttca
    28801 cgccgctgat ccccggtttt attgccgccg gtctgctgct ggggatagcg acgttaattg
    28861 ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgacgcg ctgaatttta
    28921 tgaaggtgtt cagcaaaggt ttgttcactt tcctggtgat tctggtgggc tataacgccg
    28981 cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc tttttgctcg
    29041 gttataaccc cgctgcaacc accggttact acgccggttt tcacgatttc tttggtctgc
    29101 ccatcgatcc gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc tgcgcccgca
    29161 ttgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgctg acctcgttaa
    29221 tcaccctgct gatcaccgct acgcttgcgt acctgatcat tatgccgctg ggtggctggt
    29281 tattcgaagg tatgtcatgg ctgtttatgc acctgaacag taatccgttc ggttgtgcgg
    29341 ttttagccgg gctgttcctg atcgccgtgg tatttggcgt gcatcagggc tttattcctg
    29401 tttacctcgc gttaatggac agccagggat tcaacagctt atttcccatc ctttcaatgg
    29461 caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa ccgcacagtg
    29521 cgctacgcag tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt ggtgaaccgc
    29581 tgatttacgg tgtcaccctg ccccgcatga agccgtttgt taccgcctgt ttaggcggtg
    29641 cggcgggcgg tttgtttatc ggcctgatag cctggtgggg tctgccgatg ggcttaaaca
    29701 gcgcctttgg gccgtctggt ctggtagcgc tgccgctgat gacttccgca caaggcatcc
    29761 ttccggccat ggcggtttat gctggcggga ttctggtggc atgggtttgc gggtttattt
    29821 tcaccacgct ctttggctgc cgtaacgtca atctggactg atatcatgaa acggacaatg
    29881 ctctatcttt ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata ccctgttctg
    29941 acagaaagct cgccagagaa agcaggggtt aacgtcgaac ggcttaacca gatggatcgc
    30001 tggattagcc agcaagttga tgccggttat cccggcgtaa acctgctgat cattaaagat
    30061 aatcagattg tgtatcgcaa agcctgggga gcggcgaaaa agtacgatgg cagcgtgttg
    30121 atggaacagc ctgtcaaagc caccaccggg acgctgtatg acctggcctc aaacaccaaa
    30181 atgtacgcca cgaacttcgc cctgcaaaag ctgatgagcg aaggcaaact gcatcctgac
    30241 gatcggattg cgaaatatat tccggggttt gccgatagtc caaatgacac cattaaaggc
    30301 aaaaacaccc tgcggatttc tgacctgctg catcacagcg gcggtttccc ggcagatccg
    30361 caatacccga ataaagcggt cgcgggcgcg ttatattccc aggataaagg ccagacgctg
    30421 gagatgatca agcgcacgcc gctggaatat cagcccggca gcaaacatat ctacagcgat
    30481 gtcgattata tgctgcttgg atttatcgtc gagtcggtta ccggtcagcc gcttgaccgc
    30541 tatgttgaag agtcgattta tcgcccgctc ggcctgacac atacggtatt taacccgctg
    30601 ctgaaaggct ttaaaccgca acaaattgcc gccacggaat tgaacggcaa tacccgcgat
    30661 ggcgtgatcc atttcccgaa tatccgcacc tccactctct ggggtcaggt gcacgatgaa
    30721 aaagcctttt attcgatggg cggcgtttcc gggcacgcag gtttgttttc caataccggc
    30781 gatattgcgg tgttaatgca aacgatgctg aacggcgggg gctatggcga tgtgcagctg
    30841 ttcagtgcgg aaacggtgaa gatgttcacc accagctcta aggaagatgc cacttttggc
    30901 ctcggctggc gcgtgaatgg taatgccacc atgacgccga cgtttggcac gctggcaagc
    30961 ccgcaaacct acggtcacac tggctggacc gggacagtaa ccgttatcga tccggtcaat
    31021 catatggcga ttgtgatgtt aagcaacaag ccccattcgc cagttgccga tccacaaaaa
    31081 aatcccaata tgttcgaaag cggtcagtta ccgattgcaa cttatggttg ggtagttgat
    31141 caggtgtatg cggcgttgaa gcaaaagtaa taaaaaggcc agtcagtttt gttactggct
    31201 ggcctttaat catagaatca gaaacgctta cagcgccatc agcttgtcca gcgacggcgc
    31261 gaagtaatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac gcttaccatc
    31321 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    31381 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct ggcgaacaat
    31441 cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg tttccggacg
    31501 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcctgatc
    31561 gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca caaacacata
    31621 gctgccgccg gcatccacgc cgtctttgat aaccgccact tcgcggcgcg tctcttcacc
    31681 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca cccaacggaa
    31741 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgggc
    31801 gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    31861 ggtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg ccccaacgcc
    31921 gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    31981 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    32041 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    32101 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg ccacgcaatc
    32161 cgtggcattg ttgagcgcta ttttacgtgt tttttcgcgg cggcggtttg ttcagacgca
    32221 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    32281 attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg ttgactgcct
    32341 tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca attgccaaat
    32401 gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc agcaggaata
    32461 tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat caccatcgcc
    32521 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt
    32581 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    32641 gtggacgccg ttaattcacc cacgccctgc ttgctcactt tggtggacga tccaaaccag
    32701 ttccacgggt tagcggccga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    32761 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    32821 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    32881 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc cgccactacc
    32941 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccagcaa
    33001 agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaagt gcgaggaaat
    33061 gtagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaatgcctgg aatcagcccc
    33121 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcgcgc
    33181 gacgcaccgg caatgacaaa cagcccccag gttcgcgcct gaagcaatag cacaagccag
    33241 aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg gaactcagta
    33301 ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt catatcccag
    33361 gcgttcatac attccgagca ccatgtcgtt atcttccggc acattgatct gaattttcgg
    33421 gcagccacga gcaatcagct ttttctccag ccgattaagc aacgcattgg caatcccacg
    33481 cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    33541 gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca aactgacgtc
    33601 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca gcaagtcgca
    33661 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatctccat
    33721 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    33781 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    33841 tgaaaagtaa aacaattctc aacatcaaac cgtcgtaacg gattacgcga tacgatataa
    33901 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    33961 ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg ccctgacgtt gtcaggaatg
    34021 tcgcaagcca tcgccaaaga cgaaccttta aaaaccagca acggacacag caagccgaaa
    34081 gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg cggaattgat
    34141 accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc gattgctaaa
    34201 aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg ttctggcgat
    34261 acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc agatctgttt
    34321 atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    34381 ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    34441 gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca gcaagtgctg
    34501 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgctcggctc gcatattctg
    34561 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    34621 gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat caccaacccg
    34681 gaagaagagc ggctgttagg cacggaggcg tttcgtcaga aaatcgccac agcgattgct
    34741 gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc gaaaaagcga
    34801 taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    34861 atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag ttggcagcgc
    34921 gaagctggcg gcggcgggcg tagtcgggtg ttgcgtaatg gtggtgtttt cgaacaggca
    34981 ggcgtcaact tttctcacgt ccacggcgag gcgatgccag cttccgccac cgctcatcgc
    35041 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt gcatccgcat
    35101 aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga aaaaccgggt
    35161 gcagagcccg tctggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    35221 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gcctgccatt tggtgaagac
    35281 gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca tcgcaacgaa
    35341 cagcgcggta ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    35401 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    35461 cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt
    35521 cgttatgtcg agttcaatct ggtctgggat cgcggcactc tgtttggcct gcaaactggc
    35581 gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    35641 cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt cagggattgg
    35701 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga cggcttctcg
    35761 gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc ccactgcatg
    35821 gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg gcgtacggcg
    35881 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    35941 ttttgtagcg tgcggcgact gacatgcagt tgattacaca agtccagcac cgtcaccggc
    36001 tcagacatat tttccagcac atattcacgg gcgcgggaaa gtaatctacg gtaactctga
    36061 tgactgatgc tttccgccgt caccattggt tgcgcgtctt ccagcatcgc ccccatcgcc
    36121 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttc
    36181 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgctct
    36241 ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg gttatgcaaa
    36301 aagttagcct gccgggtgat gacatcttca gaaagcacca cgcccaggat cgtgtaatca
    36361 tccggcgtgc tcagttcaaa ttcagtgcca ccagggcggg tggcgatttc cgcgcttccc
    36421 agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac
    36481 gagttcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc acgaaaaacc
    36541 tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg atgcagctga
    36601 tcgtaaatct gctgccaggc ggtaatcgtt aaagcatgtt catagacatc cgttgtctgt
    36661 cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt
    36721 tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt aatgccgggt
    36781 gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata acgactactg
    36841 cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc tttttccagc
    36901 ggccagccaa agtgggcagc cacttctcct gccgtcattc cctgacgtac tgatgttaac
    36961 agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg ttcccttacg
    37021 ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc gtctggccga
    37081 ccgatctcct tgcggctgat gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc
    37141 agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaagcg accagggtca
    37201 agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc cgctgcatct
    37261 atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat cggcaacctc
    37321 cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt aaaggcgtta
    37381 caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta gttggtttca
    37441 gacggtggcg ggacataggt caccagctgt acgtcggcag atttcaacgc tgcatcaatg
    37501 ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc aagcgtgatc
    37561 ccggcggttg atgagagata agaaccggta cgcgaaacta catgtgccag gaatgcggtg
    37621 ttttctgcgt cattagccca ctggaaagca gcgccatttt caatatgtgc aaccatcgca
    37681 tccagaccag cacgcacttc cgccgggttt ggcccaccca gcataatcag cacctcaccg
    37741 gcagtcggtg acgggccgtg tgcagcgccc gcatacagcg agcggccata caccacttcc
    37801 accatcgcct gtttggtcgc ttcatcagcc gcaatgtagg tgacgtcatc ggagtcggca
    37861 gaaatcagcc cgaggctacg aatatgcggc ggcaatttta gttcacgcgc aaattcagcg
    37921 ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc taaagctggc
    37981 atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag catacgtttc
    38041 gccaaatcca caattaccgc tgcggcttca accggcggcg tgccgccctg gtgaatgtta
    38101 gagatgcagg tgcgatcagc ctcaacggtg gtcgccatac gcggcgagta aacggcgtag
    38161 caggagaggc tttctgactg acccaacccc ggacgttccc ccaccagcag gatcaccact
    38221 ttcgcgccga ggatctcacc gatctgatct tcaatcttca cgcgaccata acgaacaaag
    38281 aatggcgtgc cgactttcag cccggcctgt ttcaggcccg ccatcagcgg cggcaggatc
    38341 tcttcgtagt tcacagtgat ggcatcagtt gacaggccat cagaaataac gacctgtacg
    38401 tccggattag caacacactg tgctttcagc gcttcaacag cttctgcaca caggcggcgg
    38461 cccatatccg ggcgagtcag gtagagattt ttgtcgctaa tttcggagcg tacttccagc
    38521 aagccctgcg ctttcaccca ctcttccggc acttctttca gtacggtatc tttcgaacga
    38581 gagtgatcgg ccaggaaacg cagcagcgcc tgcgtacgtg gacgcggacc ggcacgaccg
    38641 gtacaaacgc gggccacggt gctgcggcgc agttctgcta atacgtctgc gcgatgcggg
    38701 ttttcaacac caatccacgc ttttgcttca gcagagccta aatccagcgc gcagctttcc
    38761 gaggtcaccg gtgccgcaca gttggtggtg gcgcactttg cttctgacgg cgcgggggcc
    38821 gcttgtccca ttgacgccat cacgctgcgt acaatttctt caatctgttt ttgatccatg
    38881 atgtgttatc tccgcgtcat cagaagaaca gtgacggatc gcccgcccgt ttggtcaggc
    38941 gaccgtttgc cataatgccc atgctttcca gccagcgttc aaactccggt gacgggcgca
    39001 ggttgagtaa ctgacgcaca gtggcggtat cgtggaatgc ggtggtctgg tagttgagca
    39061 tgatgtcatc acccagcggc atccccatga tgtagttgca gcctgcggtg gcgagcagga
    39121 tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg gttggtgtaa cagcagtcac
    39181 agcccataga gatgccgctc agcttgccca taaagtgatc ttctaagcca gcacggataa
    39241 tctggcggtc gttgtagaga tactccggcc caataaagcc gaccacggtg ttgacgataa
    39301 acggatcgta atgacgcgcc agcccatagt tacgtgcttc catcgtcacc tggtcagcgc
    39361 cgaagttagc gccagcggat agcgcagagc cttgtccggt ttcgaagtag aggcagtttt
    39421 ccccggcgat acggttgaac tctgcaccca ctgcgcgcgc ttcgtcgagc atcgccagtt
    39481 ccacgccaaa ctctttcagc cctttttcgc tgccacagat actctggaaa atcagtccgc
    39541 caggcgcgcc gcgacgaatc gcttcgatct gggtggtgac gtgcgccagt acgcagccct
    39601 gagttgggat gttgaatttg tcgatcacgc cgtaaatggt gtccaacacg cggcttaagt
    39661 tttccacgtc gtcagtcacc gggttaacgc cgatcaccgc atcgcccacc ccgaaggaaa
    39721 gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc acgggtgtca tttggctgca
    39781 aacgggcgct gaaggtgccc ggaataccaa tggtggtatt ggcctttttg attaccggca
    39841 ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat cttcgctacc gccgcgacca
    39901 cttccgaggt cagccctttg cgggtaaagg caatgtcgtc cacgctggtt tcatcgctca
    39961 gcacatactc acgcagttcg ctgatgctcc agtttttaat ctggttgtag gccgtttcgt
    40021 taacatcgtc ctgaatcagc cgcgtcacgc agtcatcttc ataggcaatc accggattat
    40081 tgcggatgtc cgctacggtc atttccgaca acacctgctt tgccgccacg cgctcctgtg
    40141 agcttgccgc tgcaacgccc gccagcacat cccccgaacg cagttcgttg gctttagcca
    40201 gcacctcttt tacatcctta aactgatata cattgccgaa caatgtggtc tttagtttca
    40261 taagtcgttc cctcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc
    40321 gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat
    40381 gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa
    40441 gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac
    40501 cagcgcgttg atgaccgtca gtaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc
    40561 cagcacgtac gcgtcagttt tgggatcaag atccagctga agcagcgcct gttgccaggc
    40621 actcaccaga tccgtttcat caatcgggat cgccaccggc aaattgcgca gcggcagttg
    40681 tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat
    40741 caccgtggcg cgtacggttt gcgccggaaa ctgcacattc atctcacgca ggcgcggatg
    40801 gtcatgcagc gccgttgcca gaagtgggcc aatatcggca aaacagaacg ggtcggcggg
    40861 ctggtggcga taacattcgc ccacgccgcc agaaagcgta atgatttcgg gcgtaacacc
    40921 tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat
    40981 cacttcgaca atcagcgccg ccatccgccg ggtaacctgc accagctgcg cgccggtcag
    41041 cgaacggacg tcagtgcctg cacctaaaca ctcatccaca atcatctgcc ccggtttatg
    41101 agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccaccga cgttgaggca
    41161 ggcagtgccg ctgatttttc cgacatcgaa cagagcgtag ttcgcggtgc caccgccgat
    41221 gtcgatattc agtacccgac acagccgttg ttcagaaagg gtttgcaccc cggcaccgtg
    41281 accggcgatc acggattcga ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga
    41341 ctgagagagc gccatcaccg ccgggcgagc attgcgggtt ttcgcgcttt caccggtgat
    41401 gatgatggca ccagaatcaa cgctttccgg cgcaatgccc gcagcctgat attgctctaa
    41461 aatcagcgat ttcagttccg cttcttttaa accaccctgt ttatcgacag gggtaaagaa
    41521 caccgggctt tgccagctaa tttcgcgttt aatgaattcg tagcgcggca cctgcgacac
    41581 cgccgcacgg ttaaccagtt ccaggtggga gaaaatcacc tgggtggtgg tggtgccgat
    41641 atcgataccg acgctcaata gctggcgagt gttcacgatt gtgcctccgc ttcggtttta
    41701 gtcgcggtcg cgtcttcttt tggcaccagc atcatcgcca cgccaatcgc cgttacgccg
    41761 ccgatcaact tgccgacaat catcgggaag atcatggcat tcatgttggc agcggcgaag
    41821 cctaagtggt cgcccagggc gaaagcagcg gaaacggcga aggcgcagtt gatgactttg
    41881 ccgcgggtat ccatctgctt catcatgccg aacatcggga tgttgttggc aagcgttgcc
    41941 accatgccgg ctgccgcgat gttgttcata ttcagcactt taccgacgct catcagcggt
    42001 ttttcaaacc agcgggtcag cagcagcacc atcggatacg cccctaacag aacgcaggag
    42061 atagaaccga taacttcaat ggcgcgcatc acctcaccgg gtttatcgcc aggggccata
    42121 aagataggat ccagaccggg gatcagttcc cagccaagca ggaatttcac taccgcagcg
    42181 gcaagaccga gggtgatcaa tgcaacgagg aatttggcga agatctggaa gccgttgatc
    42241 attttttccg ggatgaattt cagccccagc gccaccagaa tcgcaacaat gatcaccggg
    42301 atcatgttca tcaggatcag ggcgaaagtg aattccaccg gctgaccgtt gatctgcaca
    42361 ccggagtaca tagcaaccag accaccagca atacagccaa tcggaatggt cacaatgccc
    42421 gccagcacgc cgagcgccag ataacgacgg tcagaaggtt cgataatgcc gagcgccacc
    42481 ggaatggaaa acacaatcgt tggccccatc atcgacccaa gaattaaccc agagtatagc
    42541 cacgctgcta cgtcgccgcc cgccagctct ttggcgagga agaagccgcc catatcgcac
    42601 gccagcagcg ttccggcgaa cattgacggg tttgcgccga gcatttcgta aaccgggata
    42661 attaccggcc cgagtacgtg agccagtacc ggtgccagcg cggtcatacc gaccatcgcc
    42721 agacccagag cgcccattgc cataaagcct tcttcgaact gaccgcctga tccttcgata
    42781 cttttaccga acttaccgag gaaacgagca gaaccgccga actgcgacag gatcctgtct
    42841 acggcagcta tcagcataaa gaacatcatg atgtacatga tgatttcgtt aattcccatc
    42901 gcctttactc cctgttagtt gttatttatt agcggatgct gcgtgaagcc ttatccgccc
    42961 tacatgtgca atcccgtagg ccggataaga cgcggcaagc gtcgcatccg gcatttattg
    43021 cgccgctgcg tacaggccga caatctgctc caggctggcg gtacgcgggt tactgcgcag
    43081 acaaatatct tccagcgcgg cctgcgccca tgcgccgtaa tgcgcagatg tcgcaccaac
    43141 atcgcccagt cgtttaccaa tcccaacttc cgcaatcagc tcacttaccg cgttaatagc
    43201 gtcacgatcg tcagattttt tagttcgcag tgcccgacca atctgactaa agcgttcacg
    43261 acaaaccatc cggttaaatt ccatcaccgt tggcagcaac atggcgttcg ccagaccgtg
    43321 tggaatatgc agcgccgcgc ccggctgatg caccatcgcg tggcacaacc caagacccgc
    43381 actggaaaac gccattcccg ccatacatga agccagcaac atgctctcgc gcgcggcaag
    43441 gtcgtgaccg tagcccaccg ctttcggcag cgatttgcca atcatcgcaa tagcgccaat
    43501 cgccaggctg tcggtaaacg gtgtagcgtt cagggcgctg tatgcttcaa tggcatgggt
    43561 taacgcatca atgccggtca tcgccgtgac atgcgacggc acaccttcgg tcaatgcggc
    43621 gtcgaggatc gccacatccg gcatcagcga ggcatgggct aacacctgct tgcgcccgct
    43681 caccgcgtcg ataatcaccg ttacattggt ggtttcagag ccggttccgg cggtcgttgg
    43741 aatggcaatc agcggcaagc gcggttgcag aacgctggtt tctgacatct ctgccagcgt
    43801 gctatccggg ttcgtcacca gcaacgccac ggctttcgcc gcatccagca ccgagccgcc
    43861 gccaaatgcg atcaccccat cacagcctga ctcacgcaac tgcgccacgg ctgcacacac
    43921 gtcggtgatg cacggttcgc ccaccggaca tggccagagc gtcatggcga tgcctttaac
    43981 agccaggctg cgcgtcagcc cggcggtcat ccctgcctga tgcaaaaagc tgtctgccat
    44041 cacgaacaga tgtttcagcc cacgcgtttg cgcttgctgt ccgcaactgc tcaccgagcc
    44101 cggaccgcaa agcgtcaccg gtggaacgct aaatgttttt acccgttgca gattcagggt
    44161 atcgaacgcc tgaaagagcg cggtctgcaa ttcattttgc atatagcccc tccgctttct
    44221 ctcacccact actggcgatc gccagcgggg tcataaacaa actgtgctgc ggtaaatgca
    44281 cctgtaacgc cgggaattgt ttgcgaaaca gttccgccac gcccggttgc atacaagagc
    44341 cgcccgccag ccataaatca gtaatgccct gcccttcaat atgccgggcg acaatgtccg
    44401 ccattttttc gtacaccggt ttcaccgtag gccaaatctc ttcaccgtga ccgcgcttgt
    44461 actgctccgc ctcttccagc gaaatacggc gatttccggc gagagtcaga gagatgtgat
    44521 gtccgccggt tgcttcatcc gccgagtacg tcaccttgcc ctttttcacg atggcaatgc
    44581 cggtagtgcc gccgccgata tccactacac cggcgttgtc cagttgcagc agatccgcga
    44641 ctgcggttgg ctcatccagc acatggctca cttccagccc ggcagattcc agcacgttga
    44701 tggaaatacg cgggtccgta cctggaggaa atgaagtcgc cgcatggcta aaacgaagac
    44761 cgaattgctg ctcaagagtg tcgagatggc gacgaacaat ggtgaccgca ccgaagaaat
    44821 cccagacgat gccgtcgcgg accacatcgg cccagtcgag gcataccgcc accggctgac
    44881 cgtcgcggtc aacaaccatc gacaccacat cgcaggtgcc cagatccacc cccagccaca
    44941 gcggagattc cgtcgcggca ggcgtctggt tacacagcgt tgccgccgtt tgcagacgtg
    45001 gggtgagcca ttgttcgtcg tgcgccatcg gttactcctt aaacaatgcg aaacgcatcg
    45061 actaatacac aacgacgcag acggacaaac gtccgcgcgc tggttacccc ttcaccggtt
    45121 ggcgtggtga tggtcatggt ggtccagcct tccccgccca gccccagccc ggcaatgcac
    45181 ggtccgttct taacgaaaat gctggtatca atggcgttcg ccatctggtt catgttttcg
    45241 atattgcgcg agtgcattgc cgccgtgtgg tggcaaccgc cctccagttt caccgccagc
    45301 gcaatggcat ccgccacgtt ggcgacgcgc acgacgggca gcaccggcat catcagttcg
    45361 gtcacggcaa acggatgttc tgcagtggtt tccacaaaca gcaaacgcgt ttcttgcggc
    45421 acgttcaggc cgattgccgc cgcgattttc gccgcatcgc gaccaaccca gtcacggctg
    45481 acggtgcctt ttccgcgctc gtcgatattt ttcagcaaca ccggttgcag ctgctgcgcc
    45541 tgttctgcgg tcagtttcac cgcgtgctgg ccttccatca gacgcatcag ttcatcggct
    45601 acgctatcaa caacaatcag taccttttcg tcggcacaaa tgatgttgtt atcgaaagac
    45661 gcgcctttga cgatggactg agcagcacgg gcgaggtcag cggtttcatc caccactacc
    45721 ggcgggttgc cagcgcctgc ggcaatcaga cgtttattgg tgtgtttacg cgccgcttct
    45781 actaccgctt cgccgccggt taccaccagc aggccgatac ccggaaactt gaacaagcgt
    45841 tgcgcggttt cgatatccgg atttgccaca gtaaccagta agttttccgg cccacctgcg
    45901 gcaacaatcg cctggttgag cagcgtaatc gcccgctggg agactttttt cgccgccgga
    45961 tgcggggcaa aaatgacgct gttgcccgcg gcaatcaggc tgatggcgtt attaattacg
    46021 gttgccgccg ggttagtgga cggagttacc gaagccacca cgccccaggg cgcgttttca
    46081 attaaagtca ggccgttgtc gccggtcagc acctgcggag agagacactc aacgcctggc
    46141 gtgccgcgcg cctgagcgac gtttttggca aatttatctt caacgcggcc catgccggtt
    46201 tcactgacgg caagttccgc taaatctctg gcgtgttttt cgcctgcctc acgaatggca
    46261 gcaatggcta actggcgcat tgccacgctt tttaaccctt gctgggcgac tttggctgcc
    46321 gcaacggcgt catccaggga cgcgaaaacg cccatctcat gaacggcggc ggacggcgtg
    46381 tcactgcttt gcattttcag cagtaccgct ttcaccacct gttcaatatc ctgttgattc
    46441 atgatgttct gccttatttg tggaaaatta cctgaccgcc agacaccacc tcatcgacaa
    46501 tgccaatcac gcacagatcg accggtgacg tttcgctttt atgcgcctgg cgggcggaac
    46561 tgccactcac cagtaacacc cactccccgg ttcccgcgcc aatattgtcg atggcgacgg
    46621 cgcattgccc gtcaggatta ccttgtggat caatcatttc caccatcagc aatttgtcat
    46681 gcgccagtcc gtgatggcgt acggtacaaa caatttgtcc agtgacgact gccagtttca
    46741 tacccgcctc cgtggcgtat ttcaggtaaa agctccccct accctccgca gaaggtaaaa
    46801 tgaaaaagga gagagcgtga cgcccgaatc gacgtcacac agggtgatta caggttgctg
    46861 ctatcgcctt tcaggccgat cgggaagact tcttccaggt caccgtgcgg acgcgggata
    46921 acgtgtacgg ataccagctc gccaatacgc tgtgccgctg cggcaccggc atcggtcgcc
    46981 gctttacagg ctgcaacatc accgcgaacc atggcagtac acaggccgcc gccaatctgc
    47041 ttaacaccaa ccagcttgac gcgcgcagct ttcaccatcg cgtcagaagc ctcaatcagt
    47101 gcaaccaggc cccgggtttc gatcattcct aatgcttcca ttgtgctttc ctctttatca
    47161 gggtccagaa cgggaccgtt cattcaacca gtgtttgtaa actgctttcg cggttcactt
    47221 ttgtctgacg cggcacagct gccaccagcg ccagctcgat aatttcctgc acgctacaac
    47281 cacgagagag atcgtgcatc ggcgcggcaa gtccttgtat cagtggcccg acggcacgat
    47341 atccgccgag tcgttgtgcg attttgtaac caatatttcc ggcttccagc gacggaaaaa
    47401 ccatcacatt ggccttgccc tgtagcgggc tggcaggcgc tttttgcgcc gccacttccg
    47461 gcacgaaggc ggcgtcaaac tgtaactcgc catcaaccac cagctttggt gcgcgctcac
    47521 ggacgatttc tgtcgcctgc tggacgttgg caacgcaggg gtgacgggcg ctaccgttac
    47581 tggaaaacga cagcatcgcc acgcgcggct cttctccggt gatggcgcgc caggtttcgg
    47641 cactggcaag cgcgatatcc gccagttgcg ccgccgtcgg ctgtggcacc acgctgcaat
    47701 cggcaaagcc caacgccggg ccgctgtact gtggcaacat caggaaaatg gatgagagcg
    47761 ttttacagcc tggctgcaag ccgataatgc gtaatccggc acgcagcaca ttcgccgtgg
    47821 aagagaggtt gcccgcgata cagacatccg ctttaccggc gctgaccatt gctgcggcga
    47881 acatcagcgg atcggtaagt ttttccagcg catccggcgg cgttttttcg cccgcgcggg
    47941 ccagccagcg atgagcaaat tcttcccgca ttgcgaggtt gccatgcgga tcaatcacct
    48001 gtagcccgtc catcgccacg ccgtgactga gcgcaaactg acgaagttca aacggattgg
    48061 cgaccagaat gggcgttgcc agacattgtt gatgtaaata ttgcgcagct ttcagcacac
    48121 gttgatctaa cgcatccgga aaaactactc tggcgggcgc tcgcaacgcc agttcacgac
    48181 aacgttcaat aatcattgcg tttcccccag tcgttgttgg atctgcttca ccgttagcgg
    48241 ctgcttcgtt acgctcagga tcatcatcac gtagaccgtg ctggaaaggc ggttcagcgc
    48301 ttgcaaaatg tccgggcgta atacttcaaa gctgtgggtg ataaagacct gcgccgctac
    48361 ggtttcggtt tcccgcactt tggtgcgtag cagattgagc aacgcagcat cgcgcccgtg
    48421 gctggcttcc ggcaccagat gatcgtggtc gagatagcgc agcggctgat gagaaagccg
    48481 atgcagatct tcgtcagaaa gcccgacaat cgcctggcag cccagcggtt cccccagcgc
    48541 atcggcgcgc ataatgttgc caagacgcga acggatatcc gccagccacg gctgccacgg
    48601 ttccgccagt tcaatttgca gccacactgc cagcgcaatg gtgctgtcta gaaccgcgcg
    48661 aaaacccaga cgcggatcgc ttttggcgac cattttttcc gccgacaggt gagtcagcgt
    48721 atccggcttt tttgccaccg gctggcggca cagttcgcag cacgcctgcg gatgttcatc
    48781 gctactggtc agcccatgaa cgggctgcgg ctgctgctgt tcatcgtcaa caaacaggcg
    48841 gccctgctcg tcaataaact tgatgcgcag atggcggctt tccagcaact cccgggcaga
    48901 gggcgtcagg cgactgtccg cagggagatg gatctctgcg ccttcgctga gcgtatggtt
    48961 cgctcttagc catgcttcgg tgatgaaatc tttcataggg attgccagtt agccggccag
    49021 gcgacataca ggaatttcac gctggatgtc gtaccaaatt cgatgctgga gcctttcggg
    49081 ataaacatca cgtcgcctgc tttggcgatc atggtttggc cttcgtggcg aacatgcagc
    49141 tccccttcca gcaccatgtc gatttcgtcg tagttcaggg tccacgggaa gaatgcgttc
    49201 tcccactgca taaaaccggc ggccatgctg ctgccgtcgt ctccggtcac aagatcagtc
    49261 aagcccacgc aatgcggctc tgcgccgtcg aaacgaccaa acttgacgct gctgccatca
    49321 atcactttga tgccgccttt gccggtcacc gatttgaagc tcggctgcat tgcgccctgc
    49381 tccagcgact gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcagta
    49441 aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg
    49501 tcggctggca cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg
    49561 atggtaaaac ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc
    49621 agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    49681 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    49741 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc
    49801 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    49861 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    49921 gtcatttgcg ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc
    49981 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt
    50041 aaattccacg gcctgtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    50101 cgttgttttt cctgccccga cgctacccac aaacgcaatt cgtttcatga atgcctcgta
    50161 ttaacttttg gtcatttcac aaagcgtata atttaataag cgacctaaac cgctgacggt
    50221 ttgtgataac gcttcttcta ctgcgcccac cgagccataa atcaccaatg caccgctgaa
    50281 gcggtcgaga aaaccgatat gcacatcagc ggctttcagc gctaaatcac cggcgatcat
    50341 cgccgtttcg ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat
    50401 tttcttcgcc agttcctcac cgggatgcgc gatgagatgc gccagtgtga cctgtttgcc
    50461 gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt
    50521 cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa
    50581 ggtgaggtag atcactaaga atggagagat attcgctgtg gtgcataaac tttcatatgc
    50641 aacgcatgaa tataaataaa ataatatcga gatattatat agactgcttt ttttattatt
    50701 cgcaaactca gtaataagct ttactaatat attgcagcca aaccgcttac cctgaatatt
    50761 cagggtaagc gtgagagtta aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg
    50821 ccagcgccac catgttgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg
    50881 gtttcgccac gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca
    50941 gtaagttgta actaatgcgg gcagcttcca tgttcggcat caccagaata ttggcagaac
    51001 ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc
    51061 cgtgcatttc accatcaatc atcagatctg gcgcacgatc cctgaccagt tccagcgcct
    51121 gacgcatttt gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag
    51181 caacgcgcgg ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg
    51241 ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac
    51301 tcggcagcag cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga
    51361 catttttcac cacgctaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat
    51421 cggcttcccc acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgccc
    51481 gctgcgcctg ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt
    51541 actctttaaa gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgctttga
    51601 tctgcaagcc cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg
    51661 gtttcgccag tcccagcgtt accagttcct gagtggcatg cagaacgcgc gcctcttccc
    51721 cttctggcag aacaacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa
    51781 acaggttggt tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa
    51841 tcggacgcgt cgccacgccc gactccatcg cggctttagc gaccgcagga gcgatcttaa
    51901 cgatcaagcg cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct
    51961 gatcgccata cgctgaagcc accacttcgc tctgttcggc gtgggccagt tccgcaatcg
    52021 cgcgtaccgc cgccagtttc atctcttcat tgatagccgt tgcgccaaca tccagcgcgc
    52081 cacggaagat aaacgggaag caaaggacgt tgttgacctg gtttggatag tcagaacgac
    52141 cggtgcaaat gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt
    52201 ccgggttcgc cagcgccagg atcattggcg cacgagccat tttcttcacc atttcctggg
    52261 tcagcacttt cgggccggaa catccaagga aaatatccgc gccttcaatc acatcatcga
    52321 gggtacgttt gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atgtttggct
    52381 cacggccctg atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac
    52441 ccagcgctac cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca
    52501 tccgcacgtc ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc
    52561 tgatgattgc cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca
    52621 gtttctgttc aatatagaaa cattctggcg ctttaatgtc ttcgaggttg attccgccga
    52681 aggtcggttc gagcgcagcg acaacttcaa taaatttgtc cgggtcgagt tcatcaactt
    52741 caatgtcaaa tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca
    52801 ccggtttgcc agccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga
    52861 tcaccgccac caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga
    52921 tttcaagaca aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg
    52981 ccagaggctt ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa
    53041 gtgcactttg ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat
    53101 aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt
    53161 ctacagactt tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct
    53221 ataacactga tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg
    53281 atgcgtttag cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt
    53341 tcaaggagta tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc
    53401 agacagcggc gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc
    53461 ttcgctgtta ctcaaagctg ccggattatc acaatatgag catttaatag acgatgctat
    53521 cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa
    53581 actggcagtc aatttcggtg ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga
    53641 agttgatgca cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt
    53701 ggacttgtat cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac
    53761 ctgggaagga attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac
    53821 gctgctgttt tcttttgccc aggcgcgggc ctgtgcagaa gcgggcgttt ttctgatttc
    53881 gccgtttgtc gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt
    53941 ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca
    54001 ccactatgaa accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt
    54061 aaccggctgc gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt
    54121 ttcgccagtg gtacgtaaat taatcccgcc ttctcagacg ttcccacgcc cagcacccat
    54181 gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc
    54241 tgaaggcatt cgtctgtttg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa
    54301 actataaacc agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg
    54361 cactcagtat ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca
    54421 tggctgatat tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa
    54481 cctggtatga tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca
    54541 gtttgctaca tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt
    54601 tgcattcgaa aaccccaggc cacccggaga ttggctatac gccaggcgtt gaaaccacca
    54661 ccggcccgct tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacac
    54721 tggcggcgca gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta
    54781 tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc
    54841 tgggactggg caagctgatt ggtttttacg atcacaacga tatttccatc gacggtgaaa
    54901 cagaaggctg gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga
    54961 tccatgaaat cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa
    55021 gcgtgaaaga taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga
    55081 ataaagcagg taaggaagag gcgcacggcg caccactggg ggaagaagaa gtggcgctgg
    55141 cacggcaaaa actgggctgg caccatccgc catttgagat ccctaaaaag atttatcacg
    55201 cctgggatgc ccgtgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg
    55261 cctataaaaa ggctcatccg caactggcag aagagtttac ccgacggatg agcggtggtt
    55321 taccgaagga ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg
    55381 cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc
    55441 ctgagttgct cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt
    55501 ctgtttcgct gaaggaagat ccagcgggca actacattca ctacggggtg cgtgaatttg
    55561 gcatgaccgc tatcgccaac ggcatcgcgc accacggcgg ctttgtgccg tataccgcga
    55621 cgttcctgat gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag
    55681 cgcggcagat tatggtttat acccacgact caattggcct gggcgaagat ggtccgacgc
    55741 accaggctgt tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac
    55801 cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac
    55861 cgacggcact gatcctctca aggcagaatc tggcccaggt ggaacgtacg ccggatcagg
    55921 ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta
    55981 ttctgattgc caccggttca gagatggaaa ttaccctgca agcggcagag aaattagcag
    56041 gagaaggtcg caatgtacgc gtagtttccc tgccctcgac cgatattttc gacgcccagg
    56101 atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag
    56161 caggtattgc cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga
    56221 cgggttacgg ggaatctgct ccggcggata agctgttccc gttctttggc tttactgccg
    56281 agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc
    56341 cggatgctga ttgccggatg cgacgctgac gcgtcttatc cggcctacag cgtcttatcc
    56401 ggcctacatg tccccgcaat tttgtttaac gggtgatcca caacgtgggc caggcgtctg
    56461 gcccatgcca gttatcgcag gtgggttctg cggcataacg caccaggcga aaacgctgac
    56521 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
    56581 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
    56641 cctgaccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagt ggctttttac
    56701 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
    56761 tcagcgcttt atcatcagtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
    56821 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgttg cgttcttcga
    56881 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
    56941 gtacgctgag cggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
    57001 tttgctgagc atcaataaac aacaacgccg cttttaagcc actcagagaa atggtctgat
    57061 tgccgtcgcg taaggtgatc gccttccctt cctgaatcat ctgcaaaaac gcggtgatgg
    57121 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaacg
    57181 ccaacggctc accatctaac agcagccgtg gcgctatctc tccttcagac gcatccggcg
    57241 acttcaaccc gccgcgctca atacgtaaaa cggcatcggt atgcgccccg gcgctgcggc
    57301 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacgcag aagttttggt
    57361 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
    57421 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
    57481 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
    57541 atcagatttg tcggattgag ccaataataa acaacgaaat attattgtta tttttcagtc
    57601 cattaaatgt gacgtttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
    57661 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
    57721 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
    57781 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
    57841 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
    57901 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
    57961 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
    58021 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
    58081 tttgcgcgtg agatcgtagg taatgttgtg cagggtgaaa tagttatcgg agagaatttt
    58141 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
    58201 tcttacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
    58261 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
    58321 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
    58381 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
    58441 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
    58501 ctggcatgac aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg
    58561 gctcgccaag acgcgtgcgc aggaaacgaa tcccgcagac gtgaccttgt tcattcagct
    58621 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcctca
    58681 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
    58741 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
    58801 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
    58861 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
    58921 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
    58981 attgttccaa aagcgaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
    59041 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
    59101 aagaaggaat gccgaaagtg agcaagccac caatttctgg atggcgatcg tacaccgtca
    59161 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccggg cctgccccga
    59221 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
    59281 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttacagca ccgtgctcat
    59341 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
    59401 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
    59461 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
    59521 gctcgccgca cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
    59581 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
    59641 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgcgccgcgg
    59701 tactggcatg ccacggttga tgttcctgac gcgcggtgcg caagcggcgg gatttcgcca
    59761 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcaacac
    59821 aggcaggacc gttttcgcgc cccgcacaaa ggtcgcattt atgcgccgtg gcttttactt
    59881 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaaaggacag gccaccacgc
    59941 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
    60001 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
    60061 cactacgttg ctgttgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
    60121 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacacccca
    60181 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
    60241 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa tcggcaatgt
    60301 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
    60361 cactgactaa attgtgtttc agctaactgg aacattaatg attttttgtg gagaagacgc
    60421 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
    60481 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    60541 ctgaggctat caatattgcc ggatcgctac gtatgcagag ttaccgcctg ggctacgact
    60601 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    60661 caccggtatt aaccaatctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    60721 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    60781 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    60841 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    60901 gtattttcac gctggtcttt tttactttgc gccgtatacg ccatcaggtg gttgccccgc
    60961 tgaatcagct ggttaccgcc agtcaacgta ttgaacacgg gcagttcgac tcgccgccgc
    61021 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac cagatgtcga
    61081 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    61141 tccacgaggc caagcgtcgt ctggaggtgt tgtatcaatg ttcccaggcg ctgaacacca
    61201 gccagattga tgtgcattgt ttccgccata ttttgcagat tgttcgcgac aatgaagcgg
    61261 ctgaatatct ggagttaaat gtcggtgaaa actggcggat tagcgaaggg caaccaaacc
    61321 cggaattgcc gatgcagatt ttaccggtga caatgcaaga gacggtttac ggcgaactgc
    61381 actggcaaaa tagtcacgtt tcatcatcag aaccgctgct taacagcgtt tcgtcgatgc
    61441 tgggacgcgg tttgtacttt aatcaggcgc agaagcattt tcagcagtta ttgttgatgg
    61501 aagaacgtgc aaccatcgcc cgcgaattgc acgactcgct ggctcaggta ctttcttact
    61561 tacgtatcca gttgacgtta ctgaagcgtt cgataccgga agataacgcc accgcacaaa
    61621 gtatcatggc cgatttttcc caggcgttga atgatgctta tcggcagtta cgcgagctgt
    61681 tgactacttt ccgcctgacg ctgcagcagg cggatctccc ctccgcgttg agggaaatgc
    61741 tggatacgtt acaaaatcaa accagcgcca aactgaccct cgactgccgt ctgccaaccc
    61801 tggcgctgga tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga
    61861 atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg
    61921 gcaatcacac ggtttatatc cgtgataacg ggattggcat cggtgaaccg aaagagcccg
    61981 aaggtcatta tggtctgaat atcatgcgcg aacgcgcgga acggctgggt gggacgctga
    62041 ctttttcaca accttccggc ggcggcacgt tagtgagtat tagctttcgc tccgcggagg
    62101 gtgaggaaag tcagctgatg taatgcctcc tactgaccaa agaatacttg cacttaaggt
    62161 tcagtataaa agggcatgat aatttacatt aactcctttt tttctccacg attggctcgt
    62221 accttgccgc tacagtgaag caagtcaagc ctacaacgat acgcagaaac acgaggtcct
    62281 cttttaatgg cgaatttctt tattgatcgc cccatttttg cctgggtgct ggcaatcctg
    62341 ttgtgtctga caggtaccct ggcgattttc tcattgcccg ttgaacaata ccccgatctc
    62401 gcgccaccga atgtgcgagt gaccgctaac tatcccggcg catcggccca gacgctggaa
    62461 aacaccgtga cccaggttat cgagcaaaat atgaccggcc tcgataatct catgtatatg
    62521 tcatctcaga gcagtggcac cggtcaggca tctgtcactt taagttttaa agcaggcacc
    62581 gatccggacg aagccgtgca gcaagtacaa aaccagctgc aatcagccat gcgaaagtta
    62641 ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac caatattctg
    62701 accattgctt tcgtctctac cgatggttcg atggataaac aggatattgc cgattatgtt
    62761 gccagtaata ttcaggaccc gttaagccgc gtgaatggcg tcggggatat cgatgcctat
    62821 ggttcgcaat attccatgcg tatctggctg gacccggcga aactcaacag tttccagatg
    62881 acggctaaag atgtcactga tgccattgag tcacagaacg cgcagattgc ggttgggcaa
    62941 cttggtggta caccttccgt cgataagcag gcgctcaacg ccaccattaa cgcccagtca
    63001 ctgctgcaaa caccagaaca gttccgcgat atcaccttga gggtgaatca agacggctca
    63061 gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg cggagaaata cgattatctt
    63121 agccgcttca atggtaagcc agcctccggg ctgggggtaa aactggcctc cggcgcaaac
    63181 gaaatggcga cagcggagct ggtgctcaat cgtctcgacg agctggcgca gtatttcccg
    63241 cacggactgg aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa
    63301 gacgtggtga aaacgctgct ggaagctatc gctctggttt tcctcgttat gtatctgttc
    63361 ctgcaaaact tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga
    63421 accttctccg tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgcgatg
    63481 gtgctggcga tcggtctgct ggtggatgac gcaatcgtgg tggtggaaaa cgtcgaacgt
    63541 attatgagtg aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc
    63601 cagggggcac tggtcgggat tgcgatggtt ctttcggcgg tatttgtacc aatggccttc
    63661 ttcggcggca ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg
    63721 gtgctgtcag tactggtagc gatgatcctc actccggctc tgtgtgccac actacttaag
    63781 ccactgaaaa aaggtgagca tcacgggcaa aaaggctttt ttgcctggtt taaccagatg
    63841 tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg
    63901 cgctggattg tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg
    63961 ccgacgtcgt tcttaccgct ggaagaccgt ggcatgttta ctacctcggt acaattgccc
    64021 agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc
    64081 acccatgaaa aagacaacat catgtcggtg tttgccactg ttggttctgg ccccgggggt
    64141 aacgggcaaa acgtggcgcg aatgtttatc cgcctgaaag actggagcga acgcgacagt
    64201 aagaccggca cctcatttgc cattatcgag cgtgcaacga aggcgtttaa caagattaaa
    64261 gaagctcgcg ttatcgccag cagcccgcca gcaattagcg gtcttggtag ttctgcaggt
    64321 tttgatatgg agttgcagga ccacgctgga gcaggtcacg atgcgctgat ggcagcacgt
    64381 aaccagttgc tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc
    64441 ctcgatgaca gtccgcagtt gcagattgat atcgaccagc gtaaagctca ggcgctgggc
    64501 gttgctatcg acgatattaa cgacacactg caaaccgcct ggggttcgag ctatgtgaat
    64561 gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc
    64621 atgctgccag atgacattaa tctctggtat gtccgaaata aagatggcgg catggtgccc
    64681 ttctctgctt tcgcgacctc acgctgggag acaggctcgc cgcgtctgga acgctataac
    64741 ggttattctg cggttgagat tgttggggaa gccgcaccgg gggtcagtac cggtacggcg
    64801 atggatatta tggaatcgtt agtgaagcag ttgccaaacg gctttggtct ggagtggacg
    64861 gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc cggcgctgta cgccatttcc
    64921 ttgctggtag tattcctgtg tctggcggca ttgtatgaaa gctggtcggt accgttctcg
    64981 gtaatgctgg tcgtgccgct gggggttatc ggcgcgctgc tggcaacctg gatgcgcggg
    65041 ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa
    65101 aacgccatcc tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca cgacctgttt
    65161 gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca
    65221 tttatcttcg gcgtattgcc aatggcaacc agcacgggtg ccggttccgg tggtcagcat
    65281 gcggtgggta ctggcgtaat gggcgggatg atttcggcca ctattctggc tatttacttc
    65341 gtgccgctgt tctttgtact ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa
    65401 gcaataaaaa aggcgacatg ctaatgtgtc gcctttttca actttccgat taagaacctg
    65461 ctcagcgggt tcttgctgtt tgtactttgt ctcaggaatt acttacgaag cataacttca
    65521 ataaagtctt tccagttccc cagttcacgt tcaatcataa caacctctct tataattatg
    65581 ggtattctac ggaaacaata taccgtggtg aagctaattt actcgattgc tgcgatgact
    65641 acctccgggg gacaaacctt atgtaaatac tatggtcctg cggtgataat ttgtatgtga
    65701 tacacagcaa catttcga
//
LOCUS       DAZFAH010000005        64031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFAH010000005 DAZFAH010000000
VERSION     DAZFAH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.005"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            187..1122
                     /locus_tag="ACHJWB_000264"
     CDS             187..1122
                     /locus_tag="ACHJWB_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4311959.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1178..1720)
                     /locus_tag="ACHJWB_000265"
     CDS             complement(1178..1720)
                     /locus_tag="ACHJWB_000265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4311960.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1704..2015)
                     /locus_tag="ACHJWB_000266"
     CDS             complement(1704..2015)
                     /locus_tag="ACHJWB_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4311961.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2200..3090)
                     /gene="cyoE"
                     /locus_tag="ACHJWB_000267"
     CDS             complement(2200..3090)
                     /gene="cyoE"
                     /locus_tag="ACHJWB_000267"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4311962.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3102..3431)
                     /gene="cyoD"
                     /locus_tag="ACHJWB_000268"
     CDS             complement(3102..3431)
                     /gene="cyoD"
                     /locus_tag="ACHJWB_000268"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4311963.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3431..4045)
                     /gene="cyoC"
                     /locus_tag="ACHJWB_000269"
     CDS             complement(3431..4045)
                     /gene="cyoC"
                     /locus_tag="ACHJWB_000269"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4311964.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAITMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4035..6026)
                     /gene="cyoB"
                     /locus_tag="ACHJWB_000270"
     CDS             complement(4035..6026)
                     /gene="cyoB"
                     /locus_tag="ACHJWB_000270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4311965.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6048..6995)
                     /gene="cyoA"
                     /locus_tag="ACHJWB_000271"
     CDS             complement(6048..6995)
                     /gene="cyoA"
                     /locus_tag="ACHJWB_000271"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4311966.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7456..8931)
                     /gene="ampG"
                     /locus_tag="ACHJWB_000272"
     CDS             complement(7456..8931)
                     /gene="ampG"
                     /locus_tag="ACHJWB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4311967.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSAAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8975..9553)
                     /gene="yajG"
                     /locus_tag="ACHJWB_000273"
     CDS             complement(8975..9553)
                     /gene="yajG"
                     /locus_tag="ACHJWB_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4311968.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9858..10175
                     /gene="bolA"
                     /locus_tag="ACHJWB_000274"
     CDS             9858..10175
                     /gene="bolA"
                     /locus_tag="ACHJWB_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4311969.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10519..11817
                     /gene="tig"
                     /locus_tag="ACHJWB_000275"
     CDS             10519..11817
                     /gene="tig"
                     /locus_tag="ACHJWB_000275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4311970.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            12063..12686
                     /gene="clpP"
                     /locus_tag="ACHJWB_000276"
     CDS             12063..12686
                     /gene="clpP"
                     /locus_tag="ACHJWB_000276"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4311971.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12812..14086
                     /gene="clpX"
                     /locus_tag="ACHJWB_000277"
     CDS             12812..14086
                     /gene="clpX"
                     /locus_tag="ACHJWB_000277"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4311972.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14274..16628
                     /gene="lon"
                     /locus_tag="ACHJWB_000278"
     CDS             14274..16628
                     /gene="lon"
                     /locus_tag="ACHJWB_000278"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4311973.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16819..17109
                     /gene="hupB"
                     /locus_tag="ACHJWB_000279"
     CDS             16819..17109
                     /gene="hupB"
                     /locus_tag="ACHJWB_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4311974.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17301..19172
                     /gene="ppiD"
                     /locus_tag="ACHJWB_000280"
     CDS             17301..19172
                     /gene="ppiD"
                     /locus_tag="ACHJWB_000280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4311975.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKLDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19323..19694
                     /gene="ybaV"
                     /locus_tag="ACHJWB_000281"
     CDS             19323..19694
                     /gene="ybaV"
                     /locus_tag="ACHJWB_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4311976.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19788..20186
                     /gene="fadM"
                     /locus_tag="ACHJWB_000282"
     CDS             19788..20186
                     /gene="fadM"
                     /locus_tag="ACHJWB_000282"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4311977.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20238..20933)
                     /gene="queC"
                     /locus_tag="ACHJWB_000283"
     CDS             complement(20238..20933)
                     /gene="queC"
                     /locus_tag="ACHJWB_000283"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4311978.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20998..22698)
                     /gene="ybaE"
                     /locus_tag="ACHJWB_000284"
     CDS             complement(20998..22698)
                     /gene="ybaE"
                     /locus_tag="ACHJWB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4311979.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPSFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22798..23616
                     /gene="cof"
                     /locus_tag="ACHJWB_000285"
     CDS             22798..23616
                     /gene="cof"
                     /locus_tag="ACHJWB_000285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4311980.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23769..24227
                     /gene="decR"
                     /locus_tag="ACHJWB_000286"
     CDS             23769..24227
                     /gene="decR"
                     /locus_tag="ACHJWB_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4311981.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24257..26029
                     /gene="mdlA"
                     /locus_tag="ACHJWB_000287"
     CDS             24257..26029
                     /gene="mdlA"
                     /locus_tag="ACHJWB_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4311982.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            26022..27803
                     /gene="mdlB"
                     /locus_tag="ACHJWB_000288"
     CDS             26022..27803
                     /gene="mdlB"
                     /locus_tag="ACHJWB_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4311983.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27984..28322
                     /gene="glnK"
                     /locus_tag="ACHJWB_000289"
     CDS             27984..28322
                     /gene="glnK"
                     /locus_tag="ACHJWB_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4311984.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28352..29638
                     /gene="amtB"
                     /locus_tag="ACHJWB_000290"
     CDS             28352..29638
                     /gene="amtB"
                     /locus_tag="ACHJWB_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4311985.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29686..30546)
                     /gene="tesB"
                     /locus_tag="ACHJWB_000291"
     CDS             complement(29686..30546)
                     /gene="tesB"
                     /locus_tag="ACHJWB_000291"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4311986.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30764..31336
                     /gene="ybaY"
                     /locus_tag="ACHJWB_000292"
     CDS             30764..31336
                     /gene="ybaY"
                     /locus_tag="ACHJWB_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4311987.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31367..31678)
                     /locus_tag="ACHJWB_000293"
     CDS             complement(31367..31678)
                     /locus_tag="ACHJWB_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4311988.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31841..31937
                     /gene="ffs"
                     /locus_tag="ACHJWB_000294"
     ncRNA           31841..31937
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJWB_000294"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            32057..32410
                     /gene="ybaA"
                     /locus_tag="ACHJWB_000295"
     CDS             32057..32410
                     /gene="ybaA"
                     /locus_tag="ACHJWB_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4311989.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32452..34002)
                     /gene="pdeB"
                     /locus_tag="ACHJWB_000296"
     CDS             complement(32452..34002)
                     /gene="pdeB"
                     /locus_tag="ACHJWB_000296"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4311990.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(34166..34636)
                     /gene="ylaC"
                     /locus_tag="ACHJWB_000297"
     CDS             complement(34166..34636)
                     /gene="ylaC"
                     /locus_tag="ACHJWB_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4311991.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDIYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34752..35303)
                     /gene="maa"
                     /locus_tag="ACHJWB_000298"
     CDS             complement(34752..35303)
                     /gene="maa"
                     /locus_tag="ACHJWB_000298"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4311992.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEKHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APSVHIYTVTHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(35474..35692)
                     /gene="hha"
                     /locus_tag="ACHJWB_000299"
     CDS             complement(35474..35692)
                     /gene="hha"
                     /locus_tag="ACHJWB_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4311993.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35718..36092)
                     /gene="tomB"
                     /locus_tag="ACHJWB_000300"
     CDS             complement(35718..36092)
                     /gene="tomB"
                     /locus_tag="ACHJWB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4311994.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(36638..39787)
                     /gene="acrB"
                     /locus_tag="ACHJWB_000301"
     CDS             complement(36638..39787)
                     /gene="acrB"
                     /locus_tag="ACHJWB_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4311995.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(39810..41003)
                     /gene="acrA"
                     /locus_tag="ACHJWB_000302"
     CDS             complement(39810..41003)
                     /gene="acrA"
                     /locus_tag="ACHJWB_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4311996.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            41145..41792
                     /gene="acrR"
                     /locus_tag="ACHJWB_000303"
     CDS             41145..41792
                     /gene="acrR"
                     /locus_tag="ACHJWB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4311997.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            41920..45282
                     /gene="mscK"
                     /locus_tag="ACHJWB_000304"
     CDS             41920..45282
                     /gene="mscK"
                     /locus_tag="ACHJWB_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4311998.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPDKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            45343..45441
                     /locus_tag="ACHJWB_000305"
                     /pseudo
     CDS             45343..45441
                     /locus_tag="ACHJWB_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45494..45655)
                     /gene="rsmS"
                     /locus_tag="ACHJWB_000306"
     CDS             complement(45494..45655)
                     /gene="rsmS"
                     /locus_tag="ACHJWB_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4311999.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(45669..46196)
                     /gene="priC"
                     /locus_tag="ACHJWB_000307"
     CDS             complement(45669..46196)
                     /gene="priC"
                     /locus_tag="ACHJWB_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4312000.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            46266..46643
                     /gene="ybaN"
                     /locus_tag="ACHJWB_000308"
     CDS             46266..46643
                     /gene="ybaN"
                     /locus_tag="ACHJWB_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4312001.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            46796..47347
                     /gene="apt"
                     /locus_tag="ACHJWB_000309"
     CDS             46796..47347
                     /gene="apt"
                     /locus_tag="ACHJWB_000309"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4312002.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            47476..49407
                     /gene="dnaX"
                     /locus_tag="ACHJWB_000310"
     CDS             47476..49407
                     /gene="dnaX"
                     /locus_tag="ACHJWB_000310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4312003.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMVLINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    48740..48804
                     /gene="dnaX"
                     /locus_tag="ACHJWB_000310"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            49460..49789
                     /gene="ybaB"
                     /locus_tag="ACHJWB_000311"
     CDS             49460..49789
                     /gene="ybaB"
                     /locus_tag="ACHJWB_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4312004.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            49789..50394
                     /gene="recR"
                     /locus_tag="ACHJWB_000312"
     CDS             49789..50394
                     /gene="recR"
                     /locus_tag="ACHJWB_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4312005.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            50504..52378
                     /gene="htpG"
                     /locus_tag="ACHJWB_000313"
     CDS             50504..52378
                     /gene="htpG"
                     /locus_tag="ACHJWB_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4312006.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            52559..53203
                     /gene="adk"
                     /locus_tag="ACHJWB_000314"
     CDS             52559..53203
                     /gene="adk"
                     /locus_tag="ACHJWB_000314"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4312007.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            53439..54401
                     /gene="hemH"
                     /locus_tag="ACHJWB_000315"
     CDS             53439..54401
                     /gene="hemH"
                     /locus_tag="ACHJWB_000315"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4312008.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(54398..55357)
                     /gene="aes"
                     /locus_tag="ACHJWB_000316"
     CDS             complement(54398..55357)
                     /gene="aes"
                     /locus_tag="ACHJWB_000316"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4312009.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            55509..56813
                     /gene="gsk"
                     /locus_tag="ACHJWB_000317"
     CDS             55509..56813
                     /gene="gsk"
                     /locus_tag="ACHJWB_000317"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4312010.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(56946..58622)
                     /gene="ybaL"
                     /locus_tag="ACHJWB_000318"
     CDS             complement(56946..58622)
                     /gene="ybaL"
                     /locus_tag="ACHJWB_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4312011.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(58860..60080)
                     /gene="fsr"
                     /locus_tag="ACHJWB_000319"
     CDS             complement(58860..60080)
                     /gene="fsr"
                     /locus_tag="ACHJWB_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4312012.1"
                     /translation="MAMSEQPQPVAGAAASTTKARISFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFSAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVELAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            60298..61950
                     /gene="ushA"
                     /locus_tag="ACHJWB_000320"
     CDS             60298..61950
                     /gene="ushA"
                     /locus_tag="ACHJWB_000320"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4312013.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(61987..62466)
                     /gene="ybaK"
                     /locus_tag="ACHJWB_000321"
     CDS             complement(61987..62466)
                     /gene="ybaK"
                     /locus_tag="ACHJWB_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4312014.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(62670..63464)
                     /gene="ybaP"
                     /locus_tag="ACHJWB_000322"
     CDS             complement(62670..63464)
                     /gene="ybaP"
                     /locus_tag="ACHJWB_000322"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4312015.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            63602..63943
                     /gene="ybaQ"
                     /locus_tag="ACHJWB_000323"
     CDS             63602..63943
                     /gene="ybaQ"
                     /locus_tag="ACHJWB_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4312016.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
BASE COUNT        16173 a        15964 c        16892 g        15002 t
ORIGIN      
        1 tttttatctt acgaatatgc tagttttatc tcactaaata aacgatttgg tgacatggat
       61 gataatgttt caggctaaat gtgtgcttat tacttggacc tttatggcac tgtatttatt
      121 cattaaatgg atatttggtg gttaattatt atggaaatat atgacagtga ggacataaag
      181 gtatacatgt taactaaaag aatattattt tctgtaatgc tgatagtttc accaagcgtg
      241 gtggccagcg agaaagcgca tgaattgtat gacagcatat acggtggtaa acctgctccc
      301 gatgttataa atacactgca taaaatggct gaatcgggag atatcgacgc gcagagcctg
      361 ttaggctggg aatattatca gcctcgttat gataccaaac ctgatgttca ggaagcgatt
      421 aaatggttcg agttagcggc taaacaaggt gacagagaag ctccgttagc gctggggggt
      481 atctactacg acggtgagca ggtgcgggtg gattatgcca aagcgtatgc actatttaat
      541 caggccgcgc agcacggtgt gaatttagca tggtccaggt tgggcattat gtacgccaat
      601 ggtcagtatg ttgaggtaga ttgtaagaaa gcgaaggaat atcttgataa aggtgtccac
      661 atttatggtg gcccggaaga ctttctggct acttgtcgaa aagacatgat tgacagaaaa
      721 acggttgacg atacgttacc cgtgattacg gttacccgtt ctgggatgcg agataatttt
      781 ttagataaag gtttttcctg catggatagc ctcttcgcca ccaccaataa attaggcgaa
      841 gtggccaatc ttcgcgtgac attcagcatt cgtcgcccat ccggtaagga gattaaccaa
      901 acggttggct tcgcgccttt cggtttaaac cggctgaata ttagctttac tgattaccta
      961 tttggttcat ttaccagtaa ttcgtctctt attctttata aaccggaatt tgagcgaaaa
     1021 tcttgtgcaa ctgtaaggac cacaatcgtt gccgcaacgg caaccattaa cggcaaggat
     1081 gtggagttgc tgaaagcggg ggcaattgaa caaaagtggt aacgctgctc agcccccggc
     1141 tgaggcaaaa cttgccctgg cgggtggcga taataatcta agccgcgaga atatgctgaa
     1201 tcgccaaaaa cataggtaca gcaccaaaag catttggtgt cgctgaaagc tgaacaaagc
     1261 cgagacgagc ataaaaatta atggcggcag ggtcagcatc aagataaacc cctttaattg
     1321 gtaatgcctg gtgaattatt tttacatgtt caaaaaaatc acatagtagg tcctgaccaa
     1381 agccctgctt ttgatacttc cgtgctaccc ccaacatgac taatctgaca acaccaattt
     1441 ctgaaggttg tgaaccctga aggacgccag aaacgcgttg tttttccagc gaatatgccg
     1501 taaaagtaca gatgccgatc agttcaccac tttgtctgtc aataagtgct ttagccgcac
     1561 agtcgctatt acgcacactt ttctttagcg atgcgcgtac aaatttatcg ataacagggt
     1621 taccgcaatc aaactgtttt tgcccaggat aagtaatatc agactgataa ttacaggaga
     1681 ggactatggg cagagtaatc tcttcatgct tgtcgaccat cgctgttttt ccttttcatt
     1741 aacgccttga gggcgagcgt cggttgagca ggttcagcga tgagttggtt catcagttcc
     1801 cagctttgat tcgaaagctc acggcgacgt tggttatcaa gcacactttc ggcgcgttcc
     1861 atagcagcgc tcaaaataaa agcgcttaaa tctaccccaa cggcttgcgc agcttcacga
     1921 agtttgtttt tggcgtcggg actagttttg agttcgaccc gcgcattttt cagcgtttta
     1981 tcgacatcgg gtagagtggc tgtttttcca ggcattatga cctctttgat taactgaatg
     2041 aaatcgtagg gagtcgatct catacataat acggctatca accgtatgat taatttatgt
     2101 tcatctgctc actctgtcaa gacatacggt tattagccgt atctctgatc tcagacaata
     2161 aaaaaggtgc cgtagcacct ttttaataga gaggttttgt taccacacag cagccagcag
     2221 cgtatgcgag tccggtacca taaaatcaac ggacatcatc accgagaggg cagtgatggc
     2281 gatgatagag aagccgaaca gcttgcgcgc ccagattctg tcatcagcaa ctttataacc
     2341 gcgcagagcc atacctaacc accagacgct aaccgccgcg gcgaccacca gatatttata
     2401 tccagcgtaa ccgccaagag agagcatcag cgtggcaacg gcaaaggcga tgatatacag
     2461 cgtgatgtga ttcttcgcca ccgaaatgcc ttttaccact ggcaataccg gaatgtttgc
     2521 cgcctggtaa tccttaaagc ggaaaatggc gatggcatag gagtgaggca tctgccacag
     2581 gctgaagata gccagcagga tcgctgcgcc gctatcgaac tcgccggtta ctgcacagta
     2641 gccaatcacc ggcggcgcag cgccggagag cgaaccaatc aatgtgccgt agactgagtg
     2701 gcgtttcatg tacaggctat agacgccgac ataaaccaca aagcccatca cccccagcca
     2761 gcaggccagc ggattcgcgc caaaccacag cagcataaag ccagcaaagc ctagcaaggt
     2821 ggcgtacacc agcgagacag caggagagat caggccttta accagcaccc gattcttcgt
     2881 cctttccatc tttctgtcga tatccctgtc gatgtagttg ttaaacacac aacccgacgc
     2941 cacaaccagt gacaccccaa ccagcgtgta gataaacagg ggataatcaa tgctgccctt
     3001 tgaggccagc aggaatcccc caatcaccga gatcaggttg ccaaagatga tgcctggttt
     3061 cgttacttgc aggtattgct taaacatcat aaccgccgct cttagtgcat catcatgttg
     3121 tagttgaggt tccacataat ccagatggag cctacaacca ggatagcgat gattagcacg
     3181 gtgaagacaa acgccgtcat gttccagcct tcatctgatt tggtattcat gtgcaggaag
     3241 cacaccagat gcaccagaac ctgtaccact gccattgcca ggattgttcc cagaattacg
     3301 gccggagagg cagcccctgt catcaccatc cagaacggaa tcaccgtcag aatgatcgac
     3361 aggataaagc ctgtcatgta ggtttttacg ctgccatggg acgcgccgct gtgatcggta
     3421 gaatgactca ttacatcgcc cccatcagat aaacaacagt gaacacacag atccaaacca
     3481 catccaggaa gtgccagaac aggctcaggc acatgatgcg ggtacggtta gtgctggtca
     3541 ggccgcgacg ggcgatttgc accatcagca ccgccatcca gataagacca gaagtgacgt
     3601 gcagaccgtg cgtgccgacc agcgcaaaga acgctgacag gaagccgctg cgatccggac
     3661 ccatgccgtt aacaatcagg tgatggaatt catagatttc catcccgata aatccggcac
     3721 caaacaacca ggtcaacgcc agccaggaga taacctggct tttgttgttt ttgtacatgg
     3781 tgatagccgc catgccgtag gtgatggagc tgaacaacag caagaaagtt tcaaccagaa
     3841 cgaacggcag ttcgaaaatg tccttacctg tcgggccgcc tgcggtgccg ttcaccagaa
     3901 cggcataggt agcaaacaag attgagaaca gaatgcagtc gctcatcagg tagatccaaa
     3961 atccgaagat tttggttccg cctgcatcgt ggtgcccgtg ttcgtgcgcg tgggcagtcg
     4021 cgtgcgtcaa agtatcagtt gccatttttc agccctgcct tagtaatctc atcgaaatgc
     4081 tggttttcca gtttttcgat ttctgccacc ggcacgtagt aatccacgtc ctcgtcgaag
     4141 cttttcacga tccaggtgat gatcatgcct gcgaagccaa caatcgccag ccaccagata
     4201 tgccagatca tggcgaaacc gaagatggtg gagaaagctg caatgacgat acctgcaccg
     4261 ctgtttttcg gcatatgaat ttcttcatag tggtcaggct ttttatacgc ttcgcctttc
     4321 tctttcattt cccagaatgc atcacgctcg tgaacgtgcg gcacaacggc aaagttatag
     4381 aacggaggcg gggaagaggt tgcccactcc agcgtacggc caccccacgg gtcgccagtc
     4441 aggtcacggt tctggtcgcg gtcgcgaata gaaacgtaca tctgaataac gaggcagaga
     4501 atacccagcg caatcagtac tgcaccgcta gctgcaatca tcagcatggt gtggaactgc
     4561 gggtcaatct gctggctcaa acgacgggtc atgcccatga agcccagcgc atacagcggc
     4621 ataaaggcaa cgaagaagcc gatgatccag aaccagaacg cgcgtttacc ccaggtttca
     4681 ttcagtttga aaccgaacgc tttaggccac cagtaggtca tccctgcgaa gcagccgaag
     4741 accacgccgc cgatgataac gttatggaag tgcgcaatca ggaacaggct gttatgcaga
     4801 acgaagtccg cgcccggtac tgccagcagc acgccagtca tcccgcccac cgagaaagtg
     4861 acgataaaac cgatggtcca cagcatcgca gaatggaaca cgatgcggcc ctgatacatg
     4921 gtgaacagcc agttgaagat cttcaccccg gtcgggatgg cgatgatcat tgtggtgata
     4981 ccaaagaagg cgtttacgtt cgcgcccgca cccatcgtaa agaagtggtg cagccaaacg
     5041 atgaacgaca gcacggtgat acagacggtt gcccatacca gcgaggtata accaaacaga
     5101 cgtttacgcg agaaggttgc cgcaatttcg gagaacacac cgaaaacagg caggatcagg
     5161 atgtaaactt ccgggtggcc ccaggcccaa atcaggttga tgtacatcat catgttgcca
     5221 cccatatcgt tggtaaagaa atgggtgccc agatagcgat ccagggtcaa caacgcgacg
     5281 gtaaccgtca gaattgggaa ggaagcaata atcagtacgt tcgcgcacag tgatgcccag
     5341 gtaaatactg gcatcttgaa catggtcatg cccggtgcgc gcatcttcag aatggtaacg
     5401 aagaagttga taccggtaag cgtcgtacct ataccggata gctggagact ccatatccag
     5461 taatcaacac cgactcccgg actgtactct attcccgata gcggtggata ggccagccag
     5521 ccggtctgcg caaattcgcc cacgccgaga gaaacgttaa ccagaatcac accaacaaca
     5581 gtaaaccaga agcttaagtt gttgaggaac gggaacgcaa cgtcacgcgc gccgatctgc
     5641 agcggaacca ccaggttcat cagaccgata acgaaaggca tcgccacgaa gaagatcata
     5701 atcacgccgt gcgcggtgaa gatctgatcg tagtggtgag gtggcaggaa gcccgcttca
     5761 cccgccgagg caagagcctg ctggctacgc atcataatgg cgtcagcaaa accacgcagc
     5821 aacatcacaa tcgccacgat gatatacatg ataccgaggc gtttatggtc gacggaggtc
     5881 agccactctt tccacaggta ggtccactta ccgaagtaag tgatcaggcc aacgagcgcc
     5941 agacctccca aaataatgcc agcgatcgta accatgacga taggttcatg gaacgggact
     6001 gcatcaagtg ataattttcc gaacatcttt attcttcctc aaccccttta atgggcggat
     6061 tccgcgtggc tcatgtccat gccttccata ccttcgtgtg cactgtgctc accttctggc
     6121 tgggtcatgt ccatgctctt accgtgagcc ataaacttgt taatcacatc ggcaaacaag
     6181 tctggtttca cgttggagaa atattccacc tggttgtatt cgctaggcgc ggccagtttt
     6241 tcgaacgcag ccatgtcaga catggtgttc ggcgactgtt tcgcttttgc gacccattgg
     6301 tcgaattcgg cgcgatccgg tgttgcaata gctttgaact tcatgcctga gaagcccggc
     6361 ccgctgtagc tggcggagat accgtcataa gtaccgggtt cgttggcgat cagatgcagg
     6421 cgagtctgca taccggccat ggcataaatc tggctaccca gacgcggaat gaagaaggag
     6481 ttcatcacgg agttggaggt cactttgaag tacaccggag tgttcgccgg gaaagcgatt
     6541 tcattcacgg tagcaatgcc ctgttccggg tagatgaaga accatttcca gtccatggaa
     6601 accacttcga tggtaatggg cttctcgtcg tgtgccagcg gcttgctagg ctcaagagcg
     6661 tgagtggttt tccaggtcag taccgcaagg aagatgatga ttaagatagg taccgtccag
     6721 accacagctt ccactttatt ggagtgtgac cagttcgggc tgtacttagc atctttattg
     6781 ctcgcacgat acttccaggc gaaaccaaca gccatcaaga ttgcgggaat aacgacaatc
     6841 aacatcaggc caaatgccgt cagtatcagt gaacgttgct ccagaccaat ctgtcctttg
     6901 ggatctaaca gcgcagaatt acagccactg agcaatacag tgcctgcaaa taatgacaac
     6961 catcccaaac ttttattgta tttcctgagt ctcatttaac gacctcaatt ccacgggatc
     7021 tggtggcgtt taaagtgtgt gggcatttta cgggaaggtt acattactgt aaacatgatt
     7081 aaattcctgt tacttggatt tggctgtcag gtcacatatg ttgcaaaaca tatcaagttg
     7141 attttaagaa gctgttgcaa aagggcgtta taacgaaagg ggagtgcaga aggagtttgg
     7201 acgggcaatt ggtataacca atctggaata aaacaattgt ttaacaaata attatcatta
     7261 tggtgacaat tgggtggggg gaaaataaag actcattagc tgaatcgtgt aagaaaaaat
     7321 agaattcata aattgcgtca ataatttcca aacgctaagc cgcacaaaag aacaaatatt
     7381 attaagcgat actgatgttt atttagccgt aataattacg gcgagtgatt actacagcta
     7441 aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat ccagcaaacc
     7501 accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagtg caggcaggaa
     7561 agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca cggccaacag
     7621 gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat attcggtacg
     7681 ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa ccagcagcaa
     7741 aataagcccc ggtacggcag cggcggcgga gaatagatag aatgtcgacc agccgtgtgc
     7801 ttcaacaaac caacccgcca cggggccgac ataaactcgt cctacagcgg aaagcgctga
     7861 gagcagggca aattgagttg cggaaaatga cttattacac agcgtcatta acagcgcgac
     7921 aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg cgcccatgct
     7981 gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg caccttgtaa
     8041 aatgccgaaa atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa tcccaccgta
     8101 caatgcgccc acaatggtcg ccagtaagcc aagcgttttg ttaaccacgc ctacttcacc
     8161 cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg cgaatgcgtc
     8221 gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc gaccaaagaa
     8281 atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa tggtgtcggt
     8341 cggttctggt gcaagcaacg tcgcgataat acaagggatc aacagcgccg ccatcaacca
     8401 gtacatgccc tgccagccca gccatttatc tgccagccac agggctaagc cgccggaaac
     8461 cagcatccct aaacggtaac ccagcacgct gattgccgcg cccgcaccac gttcttctgc
     8521 cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggccg agcaaaaagc
     8581 gatcaccacc gccagtgccg ccatccagcg gagttgggtg ccaggttcga gaaaacccat
     8641 cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc gccgccgccc
     8701 aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa aaacgtaagc
     8761 ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga ccgtcatcca
     8821 ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac ccaggatcag
     8881 caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca tgggcgtcta
     8941 caggcccggc ttgcaccggg ccagagggca gaaattaacg cgcgttctgc ttgatgaatt
     9001 cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca ctgttaaccg
     9061 catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag ctggcacgat
     9121 agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata tccgctttgg
     9181 tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg ctaacgatga
     9241 tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc atctgttttt
     9301 ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta acgatttgat
     9361 tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca ttaatgctta
     9421 cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg gaaacttcaa
     9481 tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac gggaagagga
     9541 tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa aaattcccgc
     9601 catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc atcatgttga
     9661 cgaaataatc gcccctggca aaagaaacac tgatgcgagg cctgtgtttc aatctttaaa
     9721 tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat ttgttgttaa
     9781 gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca tctcagcgtt
     9841 gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta agggcggcgt
     9901 tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc ccagccggct
     9961 ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa cgttttctga
    10021 atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc gttcatgcgc
    10081 tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc gtctttgcct
    10141 ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac tgtcggcgct
    10201 tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt tgttttgtct
    10261 ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg tgaaaaaata
    10321 cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat aatgccgcgt
    10381 cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat tataagaaca
    10441 tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc gagcactgtg
    10501 attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct tggccgccgt
    10561 gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga gctggtcaac
    10621 gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat gaatatcgtt
    10681 gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat gagccgtaac
    10741 ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac ttatgttccg
    10801 ggcgaataca agctgggtga agacttcact tactctgtag agtttgaagt ttatccggaa
    10861 gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga agtgaccgac
    10921 gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg gaaagaaaaa
    10981 gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc tgtagacggc
    11041 gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca gggtcgtatg
    11101 atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt caccatcgac
    11161 gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc gaaattcgct
    11221 atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga attcatcaaa
    11281 cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg taaaaacatg
    11341 gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat cgaaggtctg
    11401 gtaaaagcta acgacattga cgtaccggct gcgctgattg acagcgaaat cgacgttctg
    11461 cgtcgtcagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga actgccgcgc
    11521 gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct gggcgaagtt
    11581 atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat cgaagagatg
    11641 gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa caaagaactg
    11701 atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt actggcgaaa
    11761 gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca ggcgtaattt
    11821 acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgctaggtgg tgggcttttt
    11881 tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta gcgtaacaac
    11941 aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg actagctgat
    12001 aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc caggagacgg
    12061 aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg gtgccgatgg
    12121 tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt ctacttaagg
    12181 aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg attgtggcgc
    12241 agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac attaactccc
    12301 caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt atcaagcctg
    12361 atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg ttgaccgcag
    12421 gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac caaccgttgg
    12481 gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt ctgaaagtta
    12541 aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa cagattgaac
    12601 gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac ggtctggtcg
    12661 attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct atacttatcc
    12721 agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt cgtcgtgtgc
    12781 ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag atggctcagg
    12841 caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca agctgattgc
    12901 cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca tcattcgcga
    12961 agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc cgcatgaaat
    13021 tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag tgctggcggt
    13081 cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg gtgtcgagtt
    13141 gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc tgctggctga
    13201 aacgctggcg cgcctgctgg acgtcccgtt caccatggcc gacgcaacca cgctgaccga
    13261 agccggttat gtgggcgaag acgttgaaaa catcattcag aagctgttgc agaagtgcga
    13321 ttacgacgta cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg acaagatttc
    13381 tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg tacagcaggc
    13441 actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg ggcgtaaaca
    13501 tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt gtggcggtgc
    13561 gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg gcattggttt
    13621 tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc tggcgcaggt
    13681 tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc gtctgccggt
    13741 tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca aagagccgaa
    13801 aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg atctggaatt
    13861 ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa ccggtgcccg
    13921 tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc tgccgtccat
    13981 ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa gcaaaccgtt
    14041 gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa ccattcccat
    14101 acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc tctattctcg
    14161 gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag atggcgtgaa
    14221 gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc tctatgaatc
    14281 ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg gtggtttatc
    14341 cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt ctggaagcgg
    14401 cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca acggatgagc
    14461 cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag atgctgaaac
    14521 tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt atttctgcgc
    14581 tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg ccgaccattg
    14641 atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa ggctacatca
    14701 agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc gacgatccgg
    14761 cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac aaacagtccg
    14821 ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg atggaatcgg
    14881 aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag cagatggaga
    14941 aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa gaactcggtg
    15001 aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac gcggcgaaga
    15061 tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa atgatgtctc
    15121 cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta caggtaccgt
    15181 ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc cttgataccg
    15241 accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg gttcaaagcc
    15301 gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg gtaggtaaaa
    15361 cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt atggcgctgg
    15421 gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc ggttctatgc
    15481 cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg ttcctgctcg
    15541 atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca ctgcttgaag
    15601 tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg gattacgatc
    15661 tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca ccgctgctgg
    15721 atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg aacatcgcca
    15781 aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt gagctgaccg
    15841 tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg ggcgtgcgtg
    15901 gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta ctgctcgata
    15961 agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc ggtgttcagc
    16021 gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc ggtctggcgt
    16081 ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg ggtaaaggca
    16141 aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag gcggcgttaa
    16201 cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac gaaaaacgtg
    16261 acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtcccagt gccggtattg
    16321 ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc gatgtggcaa
    16381 tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg aaagaaaaac
    16441 tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa aataaacgcg
    16501 atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct gtgaagcgca
    16561 ttgaggaagt tctgactctg gcgctacaaa atgaaccgtc tggtatgcag gttgtgactg
    16621 caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg ctaattcggg
    16681 cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc ccttattaag
    16741 gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac cttgaaggat
    16801 tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca attgatcgac
    16861 aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt agatgctatt
    16921 attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt aggttttggt
    16981 acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac cggtaaagag
    17041 atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact gaaagacgcg
    17101 gtaaactaag cgttgtcccc ggtggggatg tgacgaagtt caagggcgca tctactgatg
    17161 tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa ttgcccccgt
    17221 ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca cagtcatcta
    17281 cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac agtctcgtgc
    17341 tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg agtggttacc
    17401 tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc agccgtgggc
    17461 agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg ggcgatcaat
    17521 actccgagct ggcagcgaat gaaggctata tgaaaaccct gcgtcaacag gtgctgaatc
    17581 gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg ggtatcagtg
    17641 atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac ggcaaatttg
    17701 ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat cagtacgccc
    17761 aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc ggtaccgatt
    17821 ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa cgcgtggtgc
    17881 gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc gaacaggaga
    17941 ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa ttccgcgtga
    18001 gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg gatatccaga
    18061 gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc tacagcatca
    18121 tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat aaaggcggtg
    18181 attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt aacggcggcg
    18241 atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct ggtctgaaag
    18301 aaaaagggca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt gtacgtctgg
    18361 acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac attgcggcga
    18421 aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa gtgagcgatg
    18481 cggcaagcaa cgacacggag tctctggccg gtgcagagca agctgccggc gttaaagcca
    18541 ctcagacggg ttggtttagc aaagataacc tgccggaaga gttgaacttc aagccggttg
    18601 ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc atcaactctg
    18661 acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag cacaaaccgg
    18721 aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt cagcacaaca
    18781 aagctgaaca acaggcgaaa ctggatgctg agaaactgct ggttgatttg aaagccggca
    18841 aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa atcttaagcc
    18901 gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca gcgaaagaca
    18961 aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg gcgctggatg
    19021 aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag ggtatcaccc
    19081 agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa gaggcgaaaa
    19141 tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca aaaaaatgcg
    19201 ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga acattgccat
    19261 ttgtttactg ttttcactcc cgttaaggtg attccactgt taacaaacaa ggagaaaaca
    19321 gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt gccggaatgt
    19381 ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac ggcggtagaa accaaagcgg
    19441 aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac gaagaaggca
    19501 cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg aatggcgttg
    19561 gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg tttaaaacgg
    19621 tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt aatctggcgg
    19681 tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa gaggtcatac
    19741 cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg caaacacaaa
    19801 tcaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc cgctaccttg
    19861 aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt cagtggatga
    19921 cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt cgcccagcgg
    19981 tattaagcga cctgttaact attaccagtc agttgcagca attaaacggt aaaagcggca
    20041 tcttaagcca ggtcattaca ctggagccgg aaggacaggt ggtagcggat gcgcttatta
    20101 cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg gaattgcgcg
    20161 aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca agccgcatcc
    20221 ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc catcaccgtc
    20281 ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc cgcacaatga
    20341 ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg gactaaatcc
    20401 agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca catcagcggc
    20461 gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag tgctttcaca
    20521 aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc ggtaattacg
    20581 gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa aatattacgc
    20641 ccagggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg caccggaatg
    20701 ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc aagaacctta
    20761 tgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc tgcgcgatgt
    20821 cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa tgcctgcacc
    20881 agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt catagataat
    20941 cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac cagctattca
    21001 ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg gtgtcagtcg
    21061 cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca gcggcgtcag
    21121 cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc ggttttcctc
    21181 atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg ctggagcgtc
    21241 gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat attccggtgc
    21301 ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg tcgtgtcgtc
    21361 ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg ccagtgtcgc
    21421 ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca gcgtcaaggt
    21481 tttcggtagt ttgacttcat ccggtacttg ccagtgcgga atagtccagc ctggcagtaa
    21541 tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata aaccggattg
    21601 atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac ttttgcgcaa
    21661 cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc gttgcagctc
    21721 ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctggttccca aatctttttc
    21781 gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac gcaaatggta
    21841 ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc gaaaaggacc
    21901 agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg ccagccggtg
    21961 cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat gggtgacttc
    22021 aatatgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca gcatcaataa
    22081 tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta gggttgaacg
    22141 aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg ctaaatcgcc
    22201 aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct gcccggcgag
    22261 atgctgctcg gcacggccgg gcaaaaagct tggttgtagc ggttcgagcg ggcgatagta
    22321 gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg gctgtaacag
    22381 agtgcgcagc tcacctgggg ccagttgcgc cagctccagc acatcttgct gctttccggt
    22441 ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca gaaagcgtaa
    22501 ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg cctcctgtgc
    22561 ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg ccagttcgct
    22621 gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat actggttaag
    22681 acggttgagc aatcgcatat aaacccggaa caatattatt taactattca ctattacttc
    22741 cgtatatatc aggtgatact caatcaccat taaccgtgtc acagagtgga gaaagaaatg
    22801 gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca tcatttaggt
    22861 gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct cacttttgcc
    22921 acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct ggatgcatat
    22981 ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt acatcgtgat
    23041 gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac ccgagccagc
    23101 atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc gttgttgcag
    23161 gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat gccactcggc
    23221 agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt gcagatccag
    23281 ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga ttgcctcgaa
    23341 gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca acatttaggt
    23401 ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga aatgttaggc
    23461 agcgtcggta gcgggtttat tatgggcaat gcgatgccgc aactgcgcgc ggagctcccg
    23521 catttaccgg tgattggaca ttgccgaaat caggctgtct ctcactattt gacgcactgg
    23581 ctggactatc cacatctacc ttattccccc gaataacgag atcccttcca gcaccgggca
    23641 attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt aaaccgcaca
    23701 gaataaattg tcgtgatttc acctttaaaa tagaattaaa agagaaaaaa attctctgtg
    23761 gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag caggattgca
    23821 ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct tgctggaagc
    23881 gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg ctggatccgg
    23941 aaaaaatagg cctcggcctg accgcttttg tgctgataaa aacgcaacat cacagcagcg
    24001 aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg gggttctggc
    24061 gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg aaacgctacg
    24121 acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc acttccagct
    24181 tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata tccagaatca
    24241 ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc gtcgggaatg
    24301 gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc aactggttcc
    24361 gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta ctaccgggca
    24421 gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc tcctgcgtta
    24481 cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac tgcgtgaaga
    24541 ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc gcacgggtga
    24601 tctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgccgccg gagaaggggt
    24661 gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga tgtctacgca
    24721 aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg cgatcatgat
    24781 caagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg cgttttccag
    24841 tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag cctttggtct
    24901 ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca aaaaaaacat
    24961 gcgggtggcg cgtattgatg ctcgtttcga cccgacaatc tatatcgcga ttggtatggc
    25021 gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt taacgctggg
    25081 ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc tggcgctggc
    25141 atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc gtgcgatgct
    25201 ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc gtggcgaact
    25261 tgatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc tggaaaacgt
    25321 caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg gttccggcaa
    25381 aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg atattcgctt
    25441 tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc tggcggtagt
    25501 tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt tgggttgccc
    25561 gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac atgacgatat
    25621 tttgcgttta ccgcaaggtt acgatacaga ggtgggcgag cgcggcgtga tgctttccgg
    25681 cgggcaaaaa cagcgtatct ctattgctcg tgcgttatta gtcaacgcgg aaatcctcat
    25741 ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc tgcataacct
    25801 gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt cggcactgac
    25861 ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg gcaatcatga
    25921 tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac aactggaggc
    25981 ggcgctcgac gacgctccgg aaattcgcga ggaggccgtc gatgcgtagt tttagccaac
    26041 tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcgtaaa ccgctgggga
    26101 ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg ctgcttatca
    26161 gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg gttgcagggc
    26221 tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac gcgcagtcgc
    26281 tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac gtgatggatg
    26341 ccgcattgcg ccagccatta agcgagtttg atacccaacc cgtcgggcag gtgatttccc
    26401 gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg gcaactgtcc
    26461 tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc gactggcgaa
    26521 tggcgctggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg atataccagc
    26581 gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct agcggatatc aacgacggct
    26641 ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag gcgcgatttg
    26701 gcgaacgtat gggggaggcc agtcgttcac actatatggc caggatgcaa accctgcgcc
    26761 tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt ctttgtggct
    26821 tgttgatgct gtttggcttc tccgctagcg gcaccattga agtgggcgtg ctgtatgcgt
    26881 ttatcagcta tcttgggcga cttaacgaac cattaatcga actgaccacg caacaggcga
    26941 tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac ggaccgcgcc
    27001 agcaatatgg caatgatgat cgcccgttac agagtggcac catcgaagtc gataacgtgt
    27061 catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct gtgccttcgc
    27121 gtaactttgt ggcactggtc gggcataccg gcagtggcaa aagcaccctc gccagtttat
    27181 tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt ccattaagtt
    27241 cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat ccggtggtgc
    27301 tggcggatac cttcctcgcc aacgtgacgc tggggcgtga tatctccgaa gaacgcgtct
    27361 ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg cagcatgagt gatggtattt
    27421 atacgccgct gggcgagcag gggaataatc tctccgtcgg gcaaaagcaa ctgctggcac
    27481 tggcacgcgt gttggtggag acgccgcaaa tcctgatcct tgatgaggca accgccagca
    27541 ttgactccgg tactgaacag gcgattcaac atgctctggc ggctgtgcgt gaacatacca
    27601 cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc attctggtgc
    27661 ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg gcccagggac
    27721 gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc agcgtgcgtg
    27781 aagaagaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca aaaatgtaac
    27841 gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc tgctctcttt
    27901 ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca gaaccattac
    27961 cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa ccattcaagc
    28021 tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc gtcaccgaag
    28081 tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg gaatacagcg
    28141 tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa ctcgatgaag
    28201 tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc aaaatcttcg
    28261 tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa gcggcgctgt
    28321 aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa aaactgggct
    28381 tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg ccgataaagc
    28441 cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta ttccggggat
    28501 tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc tgacgcaggt
    28561 gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact cgctggcgtt
    28621 tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa acatcgaact
    28681 gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg gctcgtttgc
    28741 ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct tctcagctgt
    28801 gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc atatggtgtg
    28861 gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca cggtggtgca
    28921 tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac gcgtgggctt
    28981 cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta ctgccattct
    29041 ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg aaatcgcggc
    29101 actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct ggatcttcgg
    29161 tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg cgattgccgg
    29221 tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt tgattatcgg
    29281 cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct tgctgcgggt
    29341 ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg gctgtatcat
    29401 gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag gtgtgacgat
    29461 gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct ggtccggtgt
    29521 ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg taccggaaga
    29581 gcaggagcga gaagggctgg atgtcaacag tcacggcgag aatgcctata acgcgtaaca
    29641 agcactgcaa aaaatagccg gacggtttta cctccggcta tttttttaat tgtgattacg
    29701 catcacccct tcctgaacgg tcgaggcaac cagcacgccg tcctgggtat aaaactcacc
    29761 gcgcacaaag ccgcgtgcgc tggacgccga ggtgctctcc acgctataca gcagccattc
    29821 attcaaatta aacgggcgat ggaaccacat ggaatggtca atggtggcaa tctgaatccc
    29881 cggttcgaga aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt taagatcaga
    29941 agcgtaaccg agcagatact gatgaacgcg caggtcatcc ggcacgctac catttgcgcg
    30001 gatccacacc tgacgatgtg gttctgcgac gtgacctttc agtgggttat gaaactccac
    30061 cggacggact tccagcggac gatcgcagat gaatttatct ttaagcactg gcggcagcag
    30121 gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg ccatcaggcg ctggcgcgga
    30181 cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg ccgtcatata
    30241 aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc
    30301 acgcagcgtt tcgacatcat aaataatcgg cttcttacta tcgccagggc gaagaaagta
    30361 gctgtgaaac gaatgtacca gccgctcttc agggacggtc tcttttgcag catacaaggc
    30421 ctgacccacg acctggccgc caaacacctg gcgtaaacct aaatcttcac tctggccgcg
    30481 aaagagtcct tcctcaattt tttccagatt taacaatgtc agtaaatttt ttagcgcctg
    30541 actcatataa ctctccagta acaaagctgc cgcagcaagc caaagtgagt tgagtataac
    30601 gcaaatttgc tactggtccg atgggtgcaa tggtctgaat tacgggctaa ttacaggcag
    30661 aaatgcgtga tgtgtgccac acttgttgac gttactattt tgttaaccac tcttccggcg
    30721 aggaaagtta gcccgctggt gcattgataa taaggagaaa tgaatgaaac tcgtgcacat
    30781 ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa gcgcggatat
    30841 tcagacgcca gccccggctg caaatacgtc tatttcagca acacaacaac ctgctatcca
    30901 gcaaccgaat gtctccggta ccgtctggat ccggcagaaa gtcgcactgc cgcctgatgc
    30961 tgtgctgacc gtgacacttt ctgacgcgtc gttagccgat gcaccgtcaa aagtgctggc
    31021 gcagaaagcg gtgcgtaccg aaggtaaaca gtcaccattc agctttgttc tgccatttaa
    31081 tccggcagat gttcagccga acgcgcgtat tctgttgagt gcggcgatta ccgtgaatga
    31141 caaactggta tttattaccg ataccgttca gccggtgatc aacaagggcg gaactaaagc
    31201 cgacctgaca ttggtgccgg tgcagcaaac cgccgtgccg gttcaggcca gcggtggtgc
    31261 aacgactacc gtaccttcga cttcaccaac tcaggtgaat ccgtcttcgg cagttccagc
    31321 tcctacgcaa tattaagcga cgttaaccct ctccgcccgg agagggttag tagttccagc
    31381 gataacgctg caagtcgatt tgcccgcttc ccgataccat cacaccttct gccagtaatg
    31441 cctgtcgctg acgctgtaaa tccggtccgg ttagcgaaat tgtgccgtgg cgattaacca
    31501 cccggtgcca gggtaaggtg ctgccttcag ggagacgctt taacacaccg cccacctggc
    31561 gcgcggcgcg gggcgatccc gccagtttcg ccacatcacc gtaagtggtg acatagcctt
    31621 cgggaatagc ggcgacgatt tgccagacgc gttggggaaa tgaatcttct ttttccatct
    31681 tttcttcctg aggtaatttt tcagcataat ctggaaaaac gcccgagtga agtcgcattg
    31741 cgcaagaaac cagcatctgg cacgcgatgg gttgcaatta gccggggcag cagtgataat
    31801 gcgcctgcgc gttggttctc aacgctctca atgggggctc tgttggttct cccgcaacgc
    31861 tactctgttt accaggtcag gtccggaagg aagcagccaa ggcagatgac gcgtgtgccg
    31921 ggatgtagct ggcagggccc ccacccattt ctgcctccca ccgtttcgtc aaaaaaaccc
    31981 aacatggcta aactttaacc acaactgacg tcgcaagaat tgtctggctg cgcagtacgc
    32041 ttcggaggta tgtctgatga agtatgttga tggttttgtg gttgccgttc ctgccgataa
    32101 aaaggatgcc tatcgggaaa tggccgctaa ggctgcgcca ttgtttaaag agtttggcgc
    32161 gcttcgtatt gtcgaatgct gggccagcga tgtaccggat ggcaaagtga ccgattttcg
    32221 tatggcggtg aaagcggaag agaatgaaga ggttgtcttt agctggattg aatacccttc
    32281 aaaagaggtc cgcgacgctg ctaatcaaaa gatgatgtcg gacccacgga tgaaagagtt
    32341 cggcgagtcc atgccgtttg acggcaagcg aatgatctat ggcggattcg agtcaatcat
    32401 cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc agcccaaaaa ttcacaaatg
    32461 ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc ttgctgtaga gccagccctg
    32521 accgtagtgc acgccatgct ggcgtagcca ctcttcttgt ttactggttt cgattccctc
    32581 cgctaccatt ttcagtttca gtgtttttgc catttcgatg atatgcggcg tgacattttt
    32641 atattccagc gcatcaacga aagatttatc gatcttcaga atgtcgacat ccagatcctg
    32701 taaataactt aaacttgaat atcccgtacc aaaatcatca agataaattt catggcccgc
    32761 ctcccggtag cgagaaatta tcggggcgct ggttttcgga tcggcaaact cgcgttcagt
    32821 gagttcaagc gcgatctgtc tgggattaac ctgatagtga ttgatcattt cacgcagcaa
    32881 ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa tatgctgctg
    32941 tggatgctga cgcagccagt cgcccatatc ttcaaagacg cttcttataa tcagtagcgt
    33001 caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg gtgacaacca
    33061 actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt tgccattggc
    33121 taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag catcctgcag
    33181 ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg ctgccagcag
    33241 gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc ctttctgcaa
    33301 cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg gcaggatatc
    33361 gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc tggtaataat
    33421 tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc caataatggc
    33481 ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg ggtcgatcat
    33541 gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta aatcgttatg
    33601 cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg ggaaggtatc
    33661 gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga tataaaccac
    33721 ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat gggcttcgct
    33781 acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt gtgtcgccac
    33841 cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa taaacgatgt
    33901 ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata ctgaaagaat
    33961 cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat ccttattaac
    34021 acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata aggaggtaga
    34081 gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca ggcgcagagc
    34141 tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg agggtaaaaa
    34201 tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt tccttatgaa
    34261 catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt acaaagcgcg
    34321 tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata tcttcatagc
    34381 ggtagcgtgg gtagatatcg aaaaagaaga aaccattaag caggataaat aatacaacca
    34441 gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg gtggcaaaaa
    34501 aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg atactaaagc
    34561 ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc
    34621 gttgtatttc ggtcatcgtt tgccttcgag gtatctgcaa ctttccaggg aacaccctat
    34681 tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc ataacagttg
    34741 cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac gacaacgttg
    34801 tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc accaatggtc
    34861 acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat ggtgacgggt
    34921 ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt taccgtgtag
    34981 atatgaacgc ttggtgccaa catacagtta tcaccgatgc gaatagggca gacatcaagc
    35041 atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata gccatagtca
    35101 cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc agcgagaatt
    35161 tgctggcgta atgtgtgctt ttccgccagg gaatgattgt atcggtgaat aagctgacga
    35221 gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc accagcaatc
    35281 atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg gcaacacagt
    35341 aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa gatcacatag
    35401 ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta tagctccgaa
    35461 agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg tcgtatagtt
    35521 tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc gccagttcat
    35581 tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca attgtctggc
    35641 aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac atacttctac
    35701 ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt cgcattgaca
    35761 aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg catattaata
    35821 ccataactgc tgaacaacat aaaggtgtca tccagatatt cgtcgatctg ctcaatgagc
    35881 ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat atgctcaatc
    35941 agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc atgattgctt
    36001 tcttcaaggt ttgcaagaca gtcatgatac agggtttcac agagaaactt aagctgtgcg
    36061 atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag tggttgaact
    36121 aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc tcgtttgtta
    36181 gcttaactat aagccactct ttgcaggttt tcatcgcata attacgaaaa attacaatta
    36241 ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag caggtaaaag
    36301 cagtttgctc gaactgttca taacatttca ggattatcgc cagatgaaat tggtgacact
    36361 aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag aattaccgca
    36421 aataattaag aatagcctct aaatgattat ggattattaa atctattagc gctacttaat
    36481 ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat actggttttc
    36541 gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt atgcataaaa
    36601 aaggccgctt gcacggcctt agtgattaca cgttgtatca atgatgatcg acagtatggc
    36661 tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca aagaataccg
    36721 gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca ccggtaccta
    36781 ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata acgccgagga
    36841 taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca tcaagcgtcg
    36901 cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg acgataagta
    36961 tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag taaacgtcat
    37021 tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc ggaacgacca
    37081 gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac aggaatacga
    37141 caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagaggcgt tcctgatagg
    37201 acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt tgttccatca
    37261 gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt tccatggatg
    37321 gcaggccatt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa gaggagaatg
    37381 ccgagaacgg caccatctga ccatcagcag cacgaacata ccagtcgccg atatcatccg
    37441 gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga ccacggtcga
    37501 taaagtcgtt cacatagcta ccgccccatg cagcgcccag agtggtgtta atgtcattga
    37561 tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac tgcggggtat
    37621 cttccagacc gtttggacgt acgctggtca acatatcagg gtgcttcgct gcttctgcaa
    37681 gcaactggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg tcaatcagct
    37741 caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta aaggcgaaaa
    37801 ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc gcttcaactt
    37861 tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca ataccggtat
    37921 tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca acgttgttct
    37981 tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta cgttcctgcg
    38041 ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca tctggcaaga
    38101 aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc agatacagca
    38161 ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag tggtgcgtgc
    38221 tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc ccgtgatcgc
    38281 ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc aacgctacca
    38341 gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag atagcaccag
    38401 tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc gcgataccga
    38461 ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc ggcaaacctt
    38521 cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc accaacaagc
    38581 cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca aaggcggcaa
    38641 ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc aacgtcgcgc
    38701 ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct tcgaccagcg
    38761 ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac gggtaaacaa
    38821 ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg attgccgcag
    38881 cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa gccggttggc
    38941 cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc gccacatcac
    39001 gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg aactcttcag
    39061 tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct ttcaccggcg
    39121 gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg gcggtaatga
    39181 catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag atacgcatcg
    39241 cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg atggcatctt
    39301 tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca tcggtgttga
    39361 taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc ccttgctgct
    39421 gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc tgaacctgcg
    39481 cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta cccgtggagt
    39541 cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt tcgataacct
    39601 gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg gagatcgtta
    39661 ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc gccagccccc
    39721 ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg ataaagaaat
    39781 taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg ctgagcaccg
    39841 cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg gacaccagga
    39901 cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc ttcagtcacc
    39961 agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac tttgtcatcc
    40021 gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc ctgttgcggg
    40081 actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa catacccggc
    40141 agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc agtggtctga
    40201 tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat gccgtcactg
    40261 gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt cgccagttcc
    40321 tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caacgtagat cggatcaagt
    40381 tgttgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc ttccgtcacg
    40441 ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc cagattgatc
    40501 cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg ttgcgcatca
    40561 gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag tttctgataa
    40621 cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag atcacctttc
    40681 gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga gacaccagct
    40741 tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac ttgaggacga
    40801 acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat ctgcagaggt
    40861 tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg ttgggcctgt
    40921 ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac cgccagaggc
    40981 gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa attggtcaat
    41041 ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat ggtacataca
    41101 ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca cgaaaaacca
    41161 aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt cttttctcac
    41221 agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc gttacgcgcg
    41281 gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc tgggaactgt
    41341 cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct ggcgatccac
    41401 tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca gaagaacgac
    41461 gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa atggctgttg
    41521 tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa caaacgttaa
    41581 aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg gcagcaatta
    41641 ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg caatcttttg
    41701 atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat ctcctgtgcc
    41761 ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag gatttttcct
    41821 ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc ggtttgactt
    41881 tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt ccagtattac
    41941 aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt cttgttatgc
    42001 cagaacacgg cgtttgcgcg ggcgtcatcg aatggcgatc tgccgacaaa agcggacctg
    42061 caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga caaactggtg
    42121 cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat aaaagaagag
    42181 acagttcagc tacggcaaaa agtcgctgaa gcgccggata aaatgcgcca ggcgaccgcg
    42241 gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat tctgagtacg
    42301 ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt gcaaaacgca
    42361 caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca gcccgaacgc
    42421 gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag tcgtctggat
    42481 gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat gcaggctcag
    42541 caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg gaacaccgtc
    42601 ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc ccgtctggag
    42661 caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt aactgaaaaa
    42721 acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa tccgctggtg
    42781 aagcaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc gactgaaaac
    42841 ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg ggcgctgcaa
    42901 tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct gttgtctcgt
    42961 atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa catgaccaac
    43021 cgcattgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga tgcactcttc
    43081 cagagcgatg cattcgtcaa caaactggaa gaaggtcaca ccaacgaagt caacagcgaa
    43141 gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga tcaactcaac
    43201 aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca gcagcagtta
    43261 atgagtgtgt cgaaaaacct gaaatccatc ctgactcagc aaatcttttg ggtgaacagt
    43321 aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa agatgaattt
    43381 aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt tatcgctttc
    43441 ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct gggctggctg
    43501 aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga tagccagctc
    43561 aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg cctgattatt
    43621 ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact gctatggtcg
    43681 ttcagcaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa ggtactggag
    43741 aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag ccactggcgt
    43801 cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc tgtggtagca
    43861 gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat tttcttcaac
    43921 ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg tgataaagag
    43981 tcgcacacca tgcgactggt caccattacc gtgctatcga ttatcccgat tgcgttgatg
    44041 gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg ttggattgaa
    44101 accgtttatt tggtgatcat ctggaacctg ctgtaccaga cagtattgcg cggcttaagc
    44161 gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa tctggtgaaa
    44221 gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga gcaagttaac
    44281 cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt catgttctgg
    44341 gcaatttggt ctgatttgat caccgtgttc agctatctcg acagcattac gctctggcat
    44401 tacaacggta ctgaagctgg tgctgcggtg gtgaaaaacg tcaccatggg cagtctgttg
    44461 tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc tggtttactg
    44521 gaagtgctgg tgctctcgcg actgaatatg cgccagggcg cgtcgtatgc cattactacc
    44581 atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc gctgggcgtc
    44641 tcgtgggata aactccagtg gctggcggca gcattatccg taggtcttgg ttttggctta
    44701 caggagatct tcggtaactt tgtctctggt ttgatcatcc tgttcgaacg tccggtgcgt
    44761 attggtgata cggtaaccat tggtagcttc tcggggacgg taagtaagat ccgtattcgt
    44821 gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa agcgtttgtt
    44881 accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt gatccgtctc
    44941 ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa ggcggcgact
    45001 gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc atttggtgcc
    45061 agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg tagtcgtact
    45121 gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat caacattgcc
    45181 tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac ggaagtcaaa
    45241 cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa gggggcgaca
    45301 tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg cggcgtgacc
    45361 gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg tgtaggcctg
    45421 ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga catttagcgg
    45481 ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt tcataatcgc
    45541 gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt tcttccagca
    45601 gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttccagt gacatactct
    45661 ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag cgcatggcgg
    45721 cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg ctgttccacg
    45781 agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg tagccgccgc
    45841 tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg tggcgcactg
    45901 tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag ttgtgccgcc
    45961 agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaactgc atgacgaagc
    46021 gcagccagat tatcgcccgc ctcatcgaga caagcctgca gtgttgtcgc acgggtttga
    46081 aaaaggtgtc tgtcaaaacg ggcacttagc gtggcaaatt gcgctaccgg ggcacaacgc
    46141 tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt tttcacgata
    46201 agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag ctgacaacac
    46261 tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg ctgggtacgc
    46321 tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg gcctggtgct
    46381 ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg tttggcagct
    46441 atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg cgggcgattt
    46501 tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca tgggtgcgca
    46561 tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt ccggtgattg
    46621 atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt gcgtacagcc
    46681 agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac aactgttact
    46741 tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata cacttatgac
    46801 cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc aggactaccc
    46861 aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga aagcttacgc
    46921 tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca aagttgtcgg
    46981 caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg gcgttggctt
    47041 tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa cttacgacct
    47101 ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg gcgacaaagt
    47161 tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg ttaaactgat
    47221 ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt tcgatctcgg
    47281 cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc cgttcccggg
    47341 ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga tgtgcattca
    47401 gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca ccttccagcg
    47461 tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc acaaaccttt
    47521 gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt gtcgttaggg
    47581 cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac ctctatcgcc
    47641 cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc gtgcggcgtg
    47701 tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga aatcgacgcc
    47761 gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca gtacgctccg
    47821 gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc gcgccacagc
    47881 tttaacgcat tgttaaaaac ccttgaagag ccgccggagc acgttaagtt tctgctggcg
    47941 acgaccgatc cacagaaatt gccggtgacg attttgtcac gctgtctgca atttcatctc
    48001 aaggcgctgg atgtcgagca aattcgccat cagcttgagc acatcctcaa cgaagaacat
    48061 atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg cagcctgcga
    48121 gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt ttcaacccag
    48181 gcggtcagtg cgatgctggg tacgcttgac gacgatcagg cgctgtcgct ggttgaagcg
    48241 atggtcgagg ccaacggcga gcgcgtaatg gtgctgatta atgaagccgc tgcccgtggt
    48301 atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat tgcgatggta
    48361 caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg gatgcgtgaa
    48421 ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct gttgattggt
    48481 cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac gctgctgcgc
    48541 gcgctggcgt tccatccacg tatgcctctg cctgagccag aagtgccacg ccagtccttt
    48601 gcgcccgtcg cgccaacggc agtaatgacg ccaacccagg tgccgccgca accgcaatca
    48661 gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca ccagccaggt gctggcggcg
    48721 cgccagcagt tgcagcgcgt gcagggagca accaaagcaa aaaagagtga accggcagcc
    48781 gctacccgcg cgcggccggt gaataacgct gcgctggaaa gactggcttc ggtcaccgat
    48841 cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag cgccagccaa aaaagaagcg
    48901 tatcgctgga aggctaccac tccagtgatg cagcaaaaag aagtggtcgc cacgccgaag
    48961 gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac tggcggcgaa gctggcggca
    49021 gaagccattg agcgcgacgc gtgggcggca caggtgagtc aactttcgct accaaaactg
    49081 gtcgaacagg tggccttaaa tgcctggaaa gaggagagcg acaacgcagt atgtctgcat
    49141 ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac agcaaaaact ggctgaagcg
    49201 ttgagcacgt taaaaggttc aacggttgaa ctgactatcg ttgaagatga taatcccgcg
    49261 gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag aaaaacttgc gcaggcgcgc
    49321 gagtccatta ttgcggataa taatattcag accctgcgtc ggttcttcga tgcggagctg
    49381 gatgaagaaa gtatccgccc catttgatcg taagcacagc ttacgttcgt catccttaac
    49441 gtgattgaga gagaaaccta tgtttggtaa aggcggtctg ggtaacctga tgaagcaagc
    49501 ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag atcgcgcagc tggaagtcac
    49561 cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac ggtgcacaca actgccgtcg
    49621 cgtagagatc gacccgagcc tgctggaaga cgacaaagag atgctggaag acctggtggc
    49681 tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg cagaaagaaa aaatggcctc
    49741 tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg ccgttctgat gcaaaccagc
    49801 ccgctgttaa cacagcttat ggaagcactg cgctgtctgc cgggcgttgg cccgaagtcg
    49861 gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta gcggcgggat gcgtctggcg
    49921 caggcgctca cccgggcgat gtcggaaatc ggccactgcg ccgattgccg cactttcacc
    49981 gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc aggaaaacgg tcaaatctgt
    50041 gtggtggaga gtccggcgga catctacgcc attgagcaga cggggcagtt ttcaggtcgt
    50101 tattttgtgt tgatggggca tctgtcaccg ctggacggca tcggtccgga tgatatcggg
    50161 cttgatcgtc tggaacagcg tctggcagag gaaaaaatca ctgaagtgat cctcgccacc
    50221 aatcccacgg ttgaaggtga agctaccgct aactacattg ccgagctttg cgcgcaatat
    50281 gacgtggaag ccagccgaat cgctcatggc gtaccggttg gcggcgaact ggaaatggtc
    50341 gatggcacca cgctgtcaca ctcccttgcc gggcgtcata agattcgttt ttaagcaaac
    50401 gagagcagga tcacctgctc tcgcttgaaa ttattctccc ttgtccccat ctctccctca
    50461 tcctgttttt aaccttaaaa tggcattatt gaggtagacc tacatgaaag gacaagaaac
    50521 tcgtggtttt cagtcagaag tgaaacagct tctgcacctg atgatccatt ctctctattc
    50581 caataaagaa atcttcctgc gtgagcttat ctctaacgcc tccgatgcgg cggacaagct
    50641 gcgtttccgt gcgctatcta acccggacct gtacgaaggt gatggcgaac tgcgcgttcg
    50701 tgtctctttc gataaagaca agcgcacgct gaccatctcc gataacggcg tggggatgac
    50761 ccgcgacgaa gtgattgacc atctggggac tatcgctaaa tccggtacca aatcattcct
    50821 cgaatccctg ggttctgacc aggcgaaaga cagccagctg atcggtcagt ttggtgttgg
    50881 tttctactct gcgtttatcg tggccgacaa agtgaccgtg cgtactcgcg cggcaggcga
    50941 aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa ggtgaatata ccgttgccga
    51001 catcaccaaa gaagatcgtg gtactgaaat caccctgcat ctgcgtgaag gcgaagacga
    51061 gttcctcgat gactggcgcg tgcgttccat catcagcaaa tactccgacc atatcgcgct
    51121 gccggtagag atcgaaaaac gcgaagagaa agacggcgaa accgttatct cctgggagaa
    51181 aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg gaaatcaccg atgaagagta
    51241 caaagagttc tacaaacaca tcgcccacga ctttaatgat ccgctgacct ggagccacaa
    51301 ccgtgttgaa ggtaagcagg agtacaccag cctgctgtac atcccgtccc aggctccgtg
    51361 ggatatgtgg aaccgcgatc ataaacacgg cctgaaactg tatgttcagc gtgtgttcat
    51421 catggacgac gcagaacagt tcatgccgaa ctatctgcgc ttcgtgcgtg gtctgattga
    51481 ctccagcgat ctgccgctga acgtttcccg tgaaatcctc caggacagca cggtaacgcg
    51541 taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg ctggaaaaac tggcgaaaga
    51601 cgacgcggaa aaataccaga ccttctggca acagtttggc ctggtactga aagaaggtcc
    51661 ggcggaagat ttcgctaacc aggaagcgat cgccaaactg ctgcgttttg cttctactta
    51721 taccgattct tctgcgcaga ccgtgtctct ggaagactac gtttcccgca tgaaagaagg
    51781 gcaggagaaa atctactaca tcactgcaga cagctatgcg gcagcgaaga gcagcccgca
    51841 cctggaactg ctgcgtaaga aaggcatcga agttctgctg ctttccgacc gcatcgatga
    51901 gtggatgatg aactatctga ctgagttcga cggtaaaccg ttccagtcgg tgtctaaagt
    51961 tgacgagtcg ctggaaaaac tggctgacga agttgatgag agcgcgaaag aagcggagaa
    52021 agcactgact ccgttcatcg accgtgtgaa agccctgctc ggcgagcgcg tgaaagatgt
    52081 ccgtctgact caccgtctga ccgatacgcc agcgatcgtt tcgaccgacg cggacgaaat
    52141 gagcactcag atggcgaaac tgttcgctgc ggcgggccag aaagtgccag aagtgaaata
    52201 catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt gcggcagata ctgaagatga
    52261 agcgaagttc agcgagtggg tagaactgct gctggatcag gcgctgctgg cagaacgcgg
    52321 cacgctggaa gatccgaacc tgtttattcg tcgtatgaac cagctgctgg tttcctgatg
    52381 tcatgccgga tgacctttgt gtcatccggc atttttcttt tcatcatctg cactttccgc
    52441 aaattatctc gccattaacc gtttcagcct caggtgcctt tcttgaggca atcgcctgtt
    52501 ggtggtatcg tttatcgctt tttcaaaaaa ttcgacacat tttaagggga ttttcgcaat
    52561 gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg actcaggctc agttcatcat
    52621 ggagaaatat ggtattccgc aaatctccac tggcgatatg ctgcgtgctg cggtcaaatc
    52681 tggctccgag ctgggtaaac aagcaaaaga cattatggat gctggcaaac tggtcaccga
    52741 cgaactggtg atcgcgctgg ttaaagagcg cattgctcag gaagactgcc gtaatggttt
    52801 cctgttggac ggcttcccgc gtaccattcc gcaggcagac gcgatgaaag aagcgggcat
    52861 caatgttgat tacgttctgg aattcgacgt accggacgaa ctgattgttg atcgtatcgt
    52921 aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac gttaaattca atccgccgaa
    52981 agtagaaggc aaagatgacg ttaccggtga agaactgact acccgtaaag acgatcagga
    53041 agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg actgcaccgc tgatcggcta
    53101 ctactccaaa gaagcggaag cgggtaacac caaatacgcg aaagttgacg gcaccaagcc
    53161 ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc taattcgaaa gcgcgcacgg
    53221 acagtcccct cgccccctcg gggagagggt tagggtgagg ggaacaggcc cgcacaagca
    53281 aacttatcag caatctcagg ccggatattc attcggcctt ttacaaaaac ctcaatctgc
    53341 gctattcctt tttctgattt gacctctcac agcaattagt tcttcttcct cacttttccg
    53401 ctacaattat caacaagttg aatcgataag aggcggtaat gcgtcagact aaaaccggta
    53461 tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta aaacgctatc
    53521 tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta tggtggccgt
    53581 tgctgcgcgg cgtgattttg ccgctgcgct cgccgcgtgt ggcgaagctg tacgcctcgg
    53641 tctggatgga agacggctcg ccgctgatgg tttacagccg ccagcaacag caggcgctgg
    53701 cacaacgttt accggagacg cccgtagcgc tgggaatgag ctacggctcg ccatcactgg
    53761 aaagcgccgt cgatgaactc ctggcagagc atgtagatca tattgtggtg ctgccgcttt
    53821 atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca cgcattctgg
    53881 cgcgcaaacg tagcattccg gggatatcgt ttattcgtga ttacgccgat aaccacgatt
    53941 acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc gaaccggatc
    54001 tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc gatgattacc
    54061 cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc actggggatg gcaccggaaa
    54121 aagtgatgat gacctttcag tcgcgctttg gccgggaacc ctggctgatg ccttataccg
    54181 acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tatacaggtg atgtgcccgg
    54241 gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac cgtgaggtct
    54301 tcctcggtgc cggcgggaaa aaatatgaat atattccagc gcttaatgcc acgccggaac
    54361 atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg gtaaagaact
    54421 gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat tcgtgaataa tgcaaaaagg
    54481 catgcagcgt acctgggtag agtttgaact cacagggctg ctgatgcgcc gctaacgtct
    54541 ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca ataaaacagg
    54601 gcggaacttc gcgagtgaga tcattattaa acagacagta atacggtgac tcgcggtccg
    54661 cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt aagccatccc
    54721 agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc ccataccaca
    54781 gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc acgcaaccac aacgcactgg
    54841 cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac atattgattt
    54901 gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc tctatcgctt
    54961 gcggaaaacg cgcttcaggt gaaagggggt aatcaatacc aatcaccgta cattggctgt
    55021 agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga ataaaaccgc
    55081 ctccatgcaa ataaaatagc gtcgccgggc tatctggctg cggacaaaag agacgtgttt
    55141 ccacctgccc atattttgtt ggaaccatgt aagctctggt tgccatttct ggagcgcccg
    55201 cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc gttcccgttg
    55261 cgggccagga cggtaaatcc ggctgaagag tattcacaac ggtcttcatt tcagcagaaa
    55321 taaggtccag aacaggtagt ttgttttccg gcttcataca aaaactcctt tccaattacg
    55381 tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt gaaactggct
    55441 gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa ccacccgtaa
    55501 aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttccct gtaaatgcac
    55561 gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc tgggtcgtag
    55621 gtattgacca gacgctggtc gatattgaag cgaaagtgga tgatgaattc attgagcgtt
    55681 atggattaag cgccgggcat tcactggtga ttgaggatga cgtagccgaa gcgctttatc
    55741 aggagctaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc attggtaaca
    55801 ccatgcacaa ctactcggtg ctcgcggacg accgttcggt gctgctgggc gtcatgtgca
    55861 gcaatattga aattggcagt tatgcctatc gttacctgtg taacacttcc agccgtaccg
    55921 atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg ctgattggcg
    55981 agtccgggga acgtaccttt gctatcagtc caggccacat gaaccagctg cgggctgaaa
    56041 gcattccgga agatgtgatt gccggagcct cggcactggt tctcacctca tatctggtgc
    56101 gttgcaagcc gggtgaaccc atgccggaag caaccatgaa agccattgag tacgcgaaga
    56161 aatataacgt accggtggtg ctgacgctgg gcaccaagtt tgtcattgcc gagaatccgc
    56221 agtggtggca gcaattcctc aaagaccacg tttctatcct tgcgatgaac gaagatgaag
    56281 ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg ctggactggg
    56341 tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc tttaccgaag
    56401 acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg cgctatagcg gaattcaacc
    56461 agtatgagtt tagccgcgcc atgcgccaca aggattgcca gaatccgctg cgtgtatatt
    56521 cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacactaat ggagcggggg
    56581 atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat cgtagcaacg
    56641 taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta gcgcaggtgt
    56701 gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct cgtttaacgc
    56761 gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt taagttatcg
    56821 tcggttcgta ggccagataa ggcgttcacg ccgcatctgg catttggctc tcgatgcccg
    56881 atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca tccggcaacc
    56941 accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc cagcatagta
    57001 cgggcaattt cacgttcacc catcactacc tgattcgcgc cacgttcggt gatatacgcc
    57061 acttcatcgt cataatgggc gcgggcaata atctcaatat ccggattttt cgcgcgggca
    57121 gatgccacaa tctcacccgc ttcataaccg ttgggaatcg tcaggatcag ccattttgca
    57181 cattccagat gcgccagttg cataatttct tcgttcgccg cattgcccaa tactgcgcgg
    57241 accccgcgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac caccagcgga
    57301 atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta acccaccagt
    57361 agcgcatggt tgcaaatatc cactgggatc tgcttctctt cttcgattgc ctcttccagc
    57421 gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc gaacagtacc
    57481 gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc ggcctgcggc
    57541 agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc aatctgcgcc
    57601 aggctggcgg cgatggttaa tgccgtacgt tgggagtgac caaacagtcg caccaggaaa
    57661 aatgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac tgccagcggt
    57721 tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa cagcaccgca
    57781 aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc agactcgttc
    57841 agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag ctctaccgca
    57901 ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc gcgagaaccg
    57961 gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag catcataatg
    58021 gcgataaatg cgatcacttt gccgatggtg atccccatat cgactgcaag agtggcaaag
    58081 cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag cgtcagaacc
    58141 attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg actgtcaatt
    58201 aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga aagacataaa
    58261 ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc catacccagc
    58321 agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt taccgccatc
    58381 aaatccttca gcgaaaagtg caaaccgacg ccgaacatca gcaaaataac gcccagttca
    58441 gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg tcctgccagc
    58501 acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc cagcatgccg
    58561 aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt ggcgtgatgc
    58621 attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac caaaattaca
    58681 ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga attttgcaga
    58741 aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac agaaggcggt ttaacagagg
    58801 aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct gcaatgaaat
    58861 cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag tggcaggaaa
    58921 gcacagattt tatagactaa ctcgatgctg gtgtgatcgg cgataagccc cagaactgcc
    58981 gctcccagac ctcccatacc aaaagcaaaa ccgaaaaaga gtccagaaac cataccgata
    59041 cgtcccggaa gcagctcctg agcgtaaacc agaatggcag agaatgccga agcgaggata
    59101 aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc
    59161 gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgccc aattttatcc
    59221 cctacaggcc cgccgatcac cgtacctgcc gcaacggcaa acaggaaggc aaacagatga
    59281 agctgagcat tctggataga taatccgaat ttttgcatca gataaaaggt gtaatagctg
    59341 ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat gctgaccgcc
    59401 agttccactt tgttgcgcgg cagtggattg ataatcgtcg ctttgggttt tcctttattc
    59461 attcggtgct gtgccgagta ccaacggctg atttgcgcca acaccacgat cgccagcagt
    59521 gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat caccgccgcc
    59581 agcaagggtc ccagggaact gccaaagttg ccgccgacct gaaagataga ttgcgccagg
    59641 ccgtgccgcc cgccggaagc catacgggct acgcgagaag attccggatg aaagaccgat
    59701 gaaccggtac cgaccagcgc cgccgccagc agaactgcgc taaaactgcc cgccagcgca
    59761 agcagcacca gaccacttaa ggtaaagcac atgccaattg gcaacgacca cggcatcgga
    59821 tatttatcgg tccagtagcc gaccactggt tgcagtagcg aagaggcgag ctggaaggtg
    59881 agggttatca tgccaatctg cataaatgtc agagaaaatt ctgactgaag cagcggataa
    59941 atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct gatagcacct
    60001 aaaataccaa acgatattcg ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg
    60061 ggttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat
    60121 cttatttgtg attattaccc gactaacata cctgtatgcg tcgtctgaag gaagtctcaa
    60181 cgccgaatac agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtc
    60241 tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga gagaagtatg
    60301 aaattattgc agcggggcgt ggcgttagcg ctgttaacca catttacact ggcgagtgaa
    60361 actgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca taccaatgat
    60421 catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg
    60481 ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc
    60541 ggtggcgaca ttaacactgg cgtgcccgag tctgacttac aggatgccga acctgatttt
    60601 cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataat
    60661 ccgctcaccg tattacgcca gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat
    60721 atctaccaga aaagtactgg cgagcgcctg tttaaaccgt gggcgctgtt taagcgtcag
    60781 gatctgaaaa ttgccgttat tgggctgaca accgatgaca cagcaaaaat tggtaacccg
    60841 gaatacttca ctgatatcga atttcgtaag cccgccgatg aagcgaagct ggtgattcag
    60901 gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac
    60961 gataatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct
    61021 gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg catggcggca
    61081 gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccaga tcaacaaaac
    61141 ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgattttgag
    61201 tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag
    61261 aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa
    61321 aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc gcagctggaa
    61381 gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta
    61441 cagaccaata tggggcggtt gattctggca gcccaaatgg atcgcactgg tgccgacttt
    61501 gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggcgatat cagctataaa
    61561 aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat gaccggtaaa
    61621 gaggtgattg attacctgac cgccgtcgcg cagatgaagc cagattcagg tgcctacccg
    61681 caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct taaaatcaaa
    61741 ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga cattaaactt caatgccacc
    61801 ggcggtgatg gatatccgcg ccttgataac aaaccgggct atgtgaatac cggctttatt
    61861 gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt gagtgtttat
    61921 gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg ccggatgttt gcatccggca
    61981 caatgcttaa tcgcggcggg cgatatcagc aaatttggca tcgaggatct ttgccagatc
    62041 gcctgccgcc agttcgatat ccagtccgcg cttgccgccg gaaacataaa tagtggcaaa
    62101 ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt ttctgcccca gtgggctaat
    62161 ccccccaacc aggtatcccg tcgaacgctg cgcgaccatc ggatcggcca tctcaacttt
    62221 cttggcaccc agcgcttttg ctactttttt aagatccagt tgacctgcga ccggcgtaac
    62281 ggccacggca aggtgtttca tatcaccgtt cactgccacc agcagcgttt tgtagacctg
    62341 atccggattc aaacctaatt ttttgacgac ttcatcgcca aaattggttt cagccggatc
    62401 gtgctcgtag gtatggattt gaaacgaaat cttgtttttt tcgagtaatt taactgcggg
    62461 tgtcatggta attttcctgc cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg
    62521 tccaaaaaaa agagtcatct tgcctaagag tattggcagg atggtgagat tgagcgacaa
    62581 tcgagttaca ccgtcgctta aagtgacggc ataataataa aaaaatgaaa ttcctctttg
    62641 acgggccaat agcgatattg gccatttttt tagcgcaaca tttgcggcaa attcccttct
    62701 ccatacaggt gtagtgcacc gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat
    62761 ttatcccgcc aggcgagatt tcgctgatgc atcagcacat cgtacagcga ctgactgaac
    62821 gtattgggca gcgttatatc attattttgc ggcggtgcat tcagccacca gctcatcatt
    62881 tgttgcagca accgtgcgtt ggtatgccag tgggtcagcg tatcgtccag cagcgccagt
    62941 cctttgtcag ggagctggag caacatggca atctggtttt cagccccttc cagttcaatc
    63001 acgggtttat gttgttgctt cgccgcctgc aatagctggt aatcgatacc gtattctgcc
    63061 cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt
    63121 tgggtagaaa agagtgaggg agaaatgccc atctcctggc taatgtgctg caggttttgg
    63181 agttgctcct cgctaatgcg ctcttccagc gcctcgcagg caggcaaatt agcaaaaggc
    63241 gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat cggcgttttt gagctttttg
    63301 agcaaacggg tgggcagggg agccatatcg tggctaccca tatgaatact gccaatcaga
    63361 tgaaaatggc gattgccggg gagggtgata tcgatggcag gccaggagta atgattaccg
    63421 cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat ccatacgacc tccctttgtg
    63481 aaatatcatg ctagcgcgcg gggagggatg gcgcaaatgg agttggggac ttgtttaatg
    63541 tgtttgtatg attcagtatg ttcttgtatc gctattcaca gggaagcaac agttaaaaac
    63601 tatgaaacag gcaacaagaa aaccgacgac acccggagat attcttctct atgaatatct
    63661 ggaaccgctc gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatag
    63721 cgtcagtaca ctgatcaata ataatcgtaa actcaccact gagatggcat tccgtctggc
    63781 gaaagttttt gataccacag tcgatttttg gctaaacctc caggcggcag ttgatctttg
    63841 ggaagttgaa aacaacatgc gcacccagga agaattggga cggattgaaa cagtggctga
    63901 atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc
    63961 atctcgtagg ccggataagg cgtttacgcc gcatccggca tccgagcacc attgcctgat
    64021 gcgacgctta c
//
LOCUS       DAZFAH010000006        63200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFAH010000006 DAZFAH010000000
VERSION     DAZFAH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.006"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..525
                     /gene="ydeJ"
                     /locus_tag="ACHJWB_000324"
     CDS             7..525
                     /gene="ydeJ"
                     /locus_tag="ACHJWB_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4312017.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(570..2615)
                     /gene="dcp"
                     /locus_tag="ACHJWB_000325"
     CDS             complement(570..2615)
                     /gene="dcp"
                     /locus_tag="ACHJWB_000325"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4312018.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2752..3498
                     /gene="ydfG"
                     /locus_tag="ACHJWB_000326"
     CDS             2752..3498
                     /gene="ydfG"
                     /locus_tag="ACHJWB_000326"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4312019.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3587..4273
                     /gene="rspR"
                     /locus_tag="ACHJWB_000327"
                     /gene_synonym="ydfH"
     CDS             3587..4273
                     /gene="rspR"
                     /locus_tag="ACHJWB_000327"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4312020.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4451..4654
                     /gene="ydfZ"
                     /locus_tag="ACHJWB_000328"
     CDS             4451..4654
                     /gene="ydfZ"
                     /locus_tag="ACHJWB_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4312021.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4689..6141)
                     /gene="ydfI"
                     /locus_tag="ACHJWB_000329"
                     /pseudo
     CDS             complement(4689..6141)
                     /gene="ydfI"
                     /locus_tag="ACHJWB_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(6230..7597)
                     /gene="ydfJ"
                     /locus_tag="ACHJWB_000330"
     CDS             complement(6230..7597)
                     /gene="ydfJ"
                     /locus_tag="ACHJWB_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO4312022.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(7655..8674)
                     /gene="rspB"
                     /locus_tag="ACHJWB_000331"
     CDS             complement(7655..8674)
                     /gene="rspB"
                     /locus_tag="ACHJWB_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO4312023.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8686..9900)
                     /gene="rspA"
                     /locus_tag="ACHJWB_000332"
     CDS             complement(8686..9900)
                     /gene="rspA"
                     /locus_tag="ACHJWB_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO4312024.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(10106..10432)
                     /gene="ynfA"
                     /locus_tag="ACHJWB_000333"
     CDS             complement(10106..10432)
                     /gene="ynfA"
                     /locus_tag="ACHJWB_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4312025.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10567..10908
                     /gene="ynfB"
                     /locus_tag="ACHJWB_000334"
     CDS             10567..10908
                     /gene="ynfB"
                     /locus_tag="ACHJWB_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4312026.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            10943..11503
                     /gene="speG"
                     /locus_tag="ACHJWB_000335"
     CDS             10943..11503
                     /gene="speG"
                     /locus_tag="ACHJWB_000335"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4312027.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11506..12216)
                     /gene="ynfC"
                     /locus_tag="ACHJWB_000336"
     CDS             complement(11506..12216)
                     /gene="ynfC"
                     /locus_tag="ACHJWB_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4312028.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            12324..12629
                     /gene="ynfD"
                     /locus_tag="ACHJWB_000337"
     CDS             12324..12629
                     /gene="ynfD"
                     /locus_tag="ACHJWB_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4312029.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12828..15254
                     /gene="ynfE"
                     /locus_tag="ACHJWB_000338"
     CDS             12828..15254
                     /gene="ynfE"
                     /locus_tag="ACHJWB_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO4312030.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNSHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVINPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            15315..17738
                     /gene="ynfF"
                     /locus_tag="ACHJWB_000339"
     CDS             15315..17738
                     /gene="ynfF"
                     /locus_tag="ACHJWB_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4312031.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETQMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSPKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17749..18366
                     /gene="ynfG"
                     /locus_tag="ACHJWB_000340"
     CDS             17749..18366
                     /gene="ynfG"
                     /locus_tag="ACHJWB_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4312032.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            18368..19222
                     /gene="ynfH"
                     /locus_tag="ACHJWB_000341"
     CDS             18368..19222
                     /gene="ynfH"
                     /locus_tag="ACHJWB_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4312033.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQASGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            19265..19879
                     /gene="dmsD"
                     /locus_tag="ACHJWB_000342"
     CDS             19265..19879
                     /gene="dmsD"
                     /locus_tag="ACHJWB_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4312034.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            20038..21330
                     /gene="clcB"
                     /locus_tag="ACHJWB_000343"
     CDS             20038..21330
                     /gene="clcB"
                     /locus_tag="ACHJWB_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4312035.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWF
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(21283..21978)
                     /gene="bioD"
                     /locus_tag="ACHJWB_000344"
     CDS             complement(21283..21978)
                     /gene="bioD"
                     /locus_tag="ACHJWB_000344"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4312036.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(22103..23323)
                     /gene="mlc"
                     /locus_tag="ACHJWB_000345"
                     /gene_synonym="dgsA"
     CDS             complement(22103..23323)
                     /gene="mlc"
                     /locus_tag="ACHJWB_000345"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4312037.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23458..24351)
                     /gene="ynfL"
                     /locus_tag="ACHJWB_000346"
     CDS             complement(23458..24351)
                     /gene="ynfL"
                     /locus_tag="ACHJWB_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4312038.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24458..25711
                     /gene="ynfM"
                     /locus_tag="ACHJWB_000347"
     CDS             24458..25711
                     /gene="ynfM"
                     /locus_tag="ACHJWB_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312039.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(25760..25879)
                     /locus_tag="ACHJWB_000348"
     CDS             complement(25760..25879)
                     /locus_tag="ACHJWB_000348"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4312040.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            26135..26443
                     /gene="asr"
                     /locus_tag="ACHJWB_000349"
     CDS             26135..26443
                     /gene="asr"
                     /locus_tag="ACHJWB_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4312041.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            26536..26619
                     /locus_tag="ACHJWB_000350"
     CDS             26536..26619
                     /locus_tag="ACHJWB_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312042.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            26719..27540
                     /gene="ydgD"
                     /locus_tag="ACHJWB_000351"
     CDS             26719..27540
                     /gene="ydgD"
                     /locus_tag="ACHJWB_000351"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4312043.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(27579..27908)
                     /gene="mdtI"
                     /locus_tag="ACHJWB_000352"
     CDS             complement(27579..27908)
                     /gene="mdtI"
                     /locus_tag="ACHJWB_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4312044.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(27895..28260)
                     /gene="mdtJ"
                     /locus_tag="ACHJWB_000353"
     CDS             complement(27895..28260)
                     /gene="mdtJ"
                     /locus_tag="ACHJWB_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4312045.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            28672..29706
                     /gene="tqsA"
                     /locus_tag="ACHJWB_000354"
     CDS             28672..29706
                     /gene="tqsA"
                     /locus_tag="ACHJWB_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO4312046.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(29731..31119)
                     /gene="pntB"
                     /locus_tag="ACHJWB_000355"
     CDS             complement(29731..31119)
                     /gene="pntB"
                     /locus_tag="ACHJWB_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4312047.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(31130..32662)
                     /gene="pntA"
                     /locus_tag="ACHJWB_000356"
     CDS             complement(31130..32662)
                     /gene="pntA"
                     /locus_tag="ACHJWB_000356"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4312048.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            33186..34130
                     /gene="ydgH"
                     /locus_tag="ACHJWB_000357"
     CDS             33186..34130
                     /gene="ydgH"
                     /locus_tag="ACHJWB_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4312049.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            34316..35698
                     /gene="ydgI"
                     /locus_tag="ACHJWB_000358"
     CDS             34316..35698
                     /gene="ydgI"
                     /locus_tag="ACHJWB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4312050.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYEPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            35735..36457
                     /gene="folM"
                     /locus_tag="ACHJWB_000359"
     CDS             35735..36457
                     /gene="folM"
                     /locus_tag="ACHJWB_000359"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4312051.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(36454..36789)
                     /gene="ydgC"
                     /locus_tag="ACHJWB_000360"
     CDS             complement(36454..36789)
                     /gene="ydgC"
                     /locus_tag="ACHJWB_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4312052.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMHLPAAFVGSVACWGISAWV
                     LIIYWIKLH"
     gene            36918..37637
                     /gene="rstA"
                     /locus_tag="ACHJWB_000361"
     CDS             36918..37637
                     /gene="rstA"
                     /locus_tag="ACHJWB_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4312053.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLVNTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            37641..38942
                     /gene="rstB"
                     /locus_tag="ACHJWB_000362"
     CDS             37641..38942
                     /gene="rstB"
                     /locus_tag="ACHJWB_000362"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4312054.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            39018..39947
                     /gene="tus"
                     /locus_tag="ACHJWB_000363"
     CDS             39018..39947
                     /gene="tus"
                     /locus_tag="ACHJWB_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4312055.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(39944..41347)
                     /gene="fumC"
                     /locus_tag="ACHJWB_000364"
     CDS             complement(39944..41347)
                     /gene="fumC"
                     /locus_tag="ACHJWB_000364"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4312056.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEYNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(41490..43136)
                     /gene="fumB"
                     /locus_tag="ACHJWB_000365"
     CDS             complement(41490..43136)
                     /gene="fumB"
                     /locus_tag="ACHJWB_000365"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4312057.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            43335..44510
                     /gene="manA"
                     /locus_tag="ACHJWB_000366"
     CDS             43335..44510
                     /gene="manA"
                     /locus_tag="ACHJWB_000366"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4312058.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            44611..46119
                     /gene="ydgA"
                     /locus_tag="ACHJWB_000367"
     CDS             44611..46119
                     /gene="ydgA"
                     /locus_tag="ACHJWB_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4312059.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(46164..47428)
                     /gene="uidC"
                     /locus_tag="ACHJWB_000368"
                     /pseudo
     CDS             complement(46164..47428)
                     /gene="uidC"
                     /locus_tag="ACHJWB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(47467..48840)
                     /gene="uidB"
                     /locus_tag="ACHJWB_000369"
     CDS             complement(47467..48840)
                     /gene="uidB"
                     /locus_tag="ACHJWB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4312060.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VAMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(48837..50648)
                     /gene="uidA"
                     /locus_tag="ACHJWB_000370"
     CDS             complement(48837..50648)
                     /gene="uidA"
                     /locus_tag="ACHJWB_000370"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO4312061.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(51037..51627)
                     /gene="uidR"
                     /locus_tag="ACHJWB_000371"
     CDS             complement(51037..51627)
                     /gene="uidR"
                     /locus_tag="ACHJWB_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4312062.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTLTEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(51856..52623)
                     /gene="hdhA"
                     /locus_tag="ACHJWB_000372"
     CDS             complement(51856..52623)
                     /gene="hdhA"
                     /locus_tag="ACHJWB_000372"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4312063.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(52735..53763)
                     /gene="malI"
                     /locus_tag="ACHJWB_000373"
     CDS             complement(52735..53763)
                     /gene="malI"
                     /locus_tag="ACHJWB_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4312064.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            53938..55530
                     /gene="malX"
                     /locus_tag="ACHJWB_000374"
     CDS             53938..55530
                     /gene="malX"
                     /locus_tag="ACHJWB_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4312065.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55540..56712
                     /gene="malY"
                     /locus_tag="ACHJWB_000375"
     CDS             55540..56712
                     /gene="malY"
                     /locus_tag="ACHJWB_000375"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4312066.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            56816..57817
                     /gene="add"
                     /locus_tag="ACHJWB_000376"
     CDS             56816..57817
                     /gene="add"
                     /locus_tag="ACHJWB_000376"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4312067.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(57852..58892)
                     /gene="ydgJ"
                     /locus_tag="ACHJWB_000377"
     CDS             complement(57852..58892)
                     /gene="ydgJ"
                     /locus_tag="ACHJWB_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4312068.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQAMELIELGI
                     ESAKHRATLCLA"
     gene            59135..59260
                     /gene="blr"
                     /locus_tag="ACHJWB_000378"
     CDS             59135..59260
                     /gene="blr"
                     /locus_tag="ACHJWB_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4312069.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            59533..59748
                     /gene="ydgT"
                     /locus_tag="ACHJWB_000379"
     CDS             59533..59748
                     /gene="ydgT"
                     /locus_tag="ACHJWB_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4312070.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            59834..60274
                     /gene="ydgK"
                     /locus_tag="ACHJWB_000380"
     CDS             59834..60274
                     /gene="ydgK"
                     /locus_tag="ACHJWB_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4312071.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            60351..60932
                     /gene="rsxA"
                     /locus_tag="ACHJWB_000381"
     CDS             60351..60932
                     /gene="rsxA"
                     /locus_tag="ACHJWB_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4312072.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            60932..61510
                     /gene="rsxB"
                     /locus_tag="ACHJWB_000382"
     CDS             60932..61510
                     /gene="rsxB"
                     /locus_tag="ACHJWB_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4312073.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            61503..>63200
                     /gene="rsxC"
                     /locus_tag="ACHJWB_000383"
     CDS             61503..>63200
                     /gene="rsxC"
                     /locus_tag="ACHJWB_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4312074.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     "
BASE COUNT        15678 a        16298 c        15359 g        15865 t
ORIGIN      
        1 aaaacagtga acattaataa agataaaatt gtacaactcg cagataccga cactattgaa
       61 aacctgacat ccgcgttgag tcaaagactt atcgcggatc aattacgctt aactaccgcc
      121 gaatcatgca ccggcggtaa gttggctagc gccctgtgtg cagctgaaga tacacccaaa
      181 ttttacggtg caggctttgt tactttcacc gatcaggcaa agatgaaaat cctcagcgta
      241 agccagcaat ctcttgaacg atattctgcg gtgagtgaga aagtggcagc agaaatggca
      301 acaggtgcca tagagcgtgc ggatgctgat gtcagtattg ccattaccgg ctacggcgga
      361 ccggagggcg gtgaagatgg tacgccagcg ggtaccgtct ggttcgcgtg gcatattaaa
      421 ggccagacct acactgcggt tatgcatttt gctggcgact gcgaaacggt attagcttta
      481 gcggtgaggt ttgccctcgt ccagctgctg caattactgc tataaccagg ctggcctggc
      541 gatatctcca ggccagccat tggtggtgtt tatatgttca agccacgatg ttgcagcatc
      601 ggcataatct gaggagcctt accgcgccat tgtcgataca ggcgttccag atcttcgctg
      661 ttacctctgg aaaggatcgc ctcgcgaaaa cgctgcccat tttcacgcgt taatccgccc
      721 tgctcaacaa accactggta accatcatcg gccaacattt gcgtccacag ataagcgtaa
      781 taacctgcag catatccgcc accaaaaata tgggcgaaat aactgctgcg atagcgtggc
      841 ggtatagcag gaagatccat attttccgcc accagcgccc gcaattcaaa atcatcgaca
      901 tcctgcattg cttcgttttc ttccaggcaa tgccagcgca tatcgagaag tgcggcgcta
      961 agcagttcgc tcatctcata ccctttgttg aacaggctgg cattacgcat tttctgttgc
     1021 agttcgtcag gcattgctgc cccgctctga taatgccggg cgtagcgagc gaatacctgc
     1081 ggatgcgttg cccagtgttc gttgatttgc gacggaaatt cgacaaaatc acgcggcgtg
     1141 ttggtgccgg aaagcgtggc ataacgctgg cgggcaaaaa ggccgtgcaa cgtatgacca
     1201 aattcatgga ataaggttat gacatcatcc cagagtaaca acgcaggctc accggcagcg
     1261 ggtttctgat aattgcaaac gttataaatt accggatgcg tttcattaag cgttgattgc
     1321 tcaacaaaat tgcccatcca tgcaccgccg ctttttgaat cacgggcgaa gaaatcaccg
     1381 taaaataacg ccagccccac gccattatga tcaaaaattt cccacacccg aacgtcagga
     1441 tggtagacag gaatatcaaa acgttcgaca aacttaatac cgaagagctg attcgcggtc
     1501 cagaatacac cttcattcaa caccgtgttt aattcaaaat atggcttgag ctgcgcctca
     1561 tcaagatcaa atttctcccg ccgtacctgt tccgcataaa atgcccagtc ccacggctgc
     1621 gcgctaaacc cgccctgctg cttatcgata accgcctgta tggaggctaa ttcatcgctc
     1681 gcacgttgac gcgccgctgg aacaatttcc cgcataaagt tgagtgctgc ttctggcgtt
     1741 tttgccatct gatcggcgat tttccatgcg gcataatgag gaaaaccaag cagtttcgcc
     1801 tgctgtgcgc gaatctctac cagacgttga atgatagcgc gggtatcatt accatcattt
     1861 ttttccgctc gcgtccagcc cgcaataaac agtttttcac gcgtcgcacg atcgcgcatt
     1921 tcggcaagcg ccggttgttg ggtggtattc agcagcggaa tcagccattt gttatccaga
     1981 cctttctcgc gagccgcctc tgccgccagc gcaatctctt gctcactcat tcctgccagc
     2041 tgcgcgatat cgttcacaac cagaccgccg gatttatttg ctgcaagtaa ccgctggtta
     2101 aactggctgg tcagggtcgc agcttctgta ttcagtactt ttaattttgc tttatcagct
     2161 tgcgcaagtt tggctccggc aaggacaaaa cgttgatgaa taacctccac caggcggatg
     2221 gattcactat caagccccag ggattcacgg cgctgccaga cagcatctac ccgcgcgaat
     2281 aattcaccgt tcagatagat atcattagcc agttccgcca gttcagcgga aaactgttca
     2341 tcaagacgct gtaattcatc attggtatgc gccgcagtca tcgcaaaaaa gacgctggta
     2401 acgcgggtaa gtaattctcc gctttgttcc agtgccagaa tagtattgtt gaaatcaggc
     2461 gtttgcgggt taagcgcgat ggcagcaatt tctgcccgct tttgctgcat tccctcatcg
     2521 aatgccgggc gatagtgatg attggcaatt tgatcaaaat gaggagccag atacggcagt
     2581 gtgctttgca caaggaaagg attcattgtt gtcattttct tctcctgaac gcgaggtgtt
     2641 ccatagcgta ggcttactga taacggagtg caatcttgca aaccagtatt acccgctctt
     2701 aagcatcacg tgctatgtta ttgacacaca aaagcgttga ggaacagtga gatgatcgtt
     2761 ttagtaactg gagcaacggc aggttttggt gaatgcatta ctcgtcgttt tattcaacaa
     2821 gggcataaag ttatcgccac tggccgtcgc caggagcggt tgcaggagtt aaaagacgaa
     2881 ctgggagata atctgtatat cgcccaactg gacgttcgca accgcgccgc tattgaagag
     2941 atgctggcat cgcttcctgc cgagtggtgc aatattgata tcctggtaaa taatgccggc
     3001 ttggcgttgg gcatggagcc tgcgcataaa gccagcgttg aagactggga aacgatgatt
     3061 gataccaaca acaaaggcct ggtatatatg acgcgcgccg tcttaccggg tatggttgaa
     3121 cgtaatcatg gtcatattat taacattggc tcaacggcag gtagctggcc gtatgccggt
     3181 ggtaacgttt acggtgcgac gaaagcgttt gttcgtcagt ttagcctgaa tctgcgtacg
     3241 gatctgcatg gtacggcggt gcgcgtcacc gacatcgaac cgggtctggt gggtggcacc
     3301 gagttttcca atgtccgctt taaaggcgat gacggtaaag cggaaaaaac ctatcaaaat
     3361 accgttgcat tgacgccaga agatgtcagc gaagccgtct ggtgggtgtc aacgctgcct
     3421 gctcacgtca atatcaatac cctggaaatg atgccggtta cccaaagcta tgccggactg
     3481 aatgtccacc gtcagtaatt tttatacccg gcgtaactgc cgggttattg cttgtcacaa
     3541 aaaagtggta gactcatgca gttaactcac tcacaagcaa gaacgaatga ccgtcgaaac
     3601 gcaacttaat cccacacagc ctgtcaatca gcaaatttat cgtatccttc gtcgcgacat
     3661 tgtccattgc ctgattgctc caggcacacc gttgtcggaa aaagaagttt ctgttcgttt
     3721 caatgtgtca cgccagccgg ttcgtgaagc ctttattaaa ctggcggaaa acggcctgat
     3781 tcaaattcgt ccacagcgtg gcagctacgt caacaaaatt tccatggccc aggtgcgcaa
     3841 cggcagtttt atccgtcagg ccattgagtg cgcggtggcg cgtcgggcgg cgagcatgat
     3901 taccgaaagc cagtgctacc aactggaaca aaatcttcac cagcaacgca ttgccattga
     3961 gcgcaagcaa ctggatgatt tttttgaact tgatgacaac ttccatcaac tcctgacgca
     4021 gattgccgac tgtcaactgg cgtgggatac cattgagaac ctgaaagcga ccgttgatcg
     4081 cgtgcgctat atgagtttcg accatgtttc tccaccagaa atgctgttac gccagcatct
     4141 tgatattttc tctgccctgc aaaaacgtga tggcgatgcg gtagaacgtg caatgacgca
     4201 acatttgcag gaaatcagcg aatccgtgcg ccagatccgc caggaaaaca gcgactggtt
     4261 tagcgaagag taattcattt actctcatcc catccgggat gagaggcttt tccccccgcc
     4321 ttacggctca tgcatgcatc aaaaaagatg tgagcttgat caaaaacaaa aaatatttca
     4381 ctcgacagga gtatttatat tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga
     4441 gaaaacacct atgacgacct acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt
     4501 tatggttagc ggcaccggtc acactggcaa gatcctgtcg attgatactg aaggtctgac
     4561 cgctgagcaa atccgccgcg gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc
     4621 acctctggac ctgattcgtc tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca
     4681 ttgctttttt aattcacgga atatttcgcc acggtcgctt tcgcaccatg cgctaataaa
     4741 gacaagtacg cttccgtcac ttttgcagta aacaagctat tatctggcaa atcatcacca
     4801 aaaatcgcct taatcgccag caatgactgg acgcgcgctt tcccttcggc actactttgt
     4861 acagccttct gaataacagg taacagtggg tcactgattt caatcggatt tccctgttca
     4921 tcaacaccac cgacataacg catccaaccc gcgacgccca gtgccagcag ctcgaacttg
     4981 ctgtcatgcg ccagatgcca gcgaacagaa tccaacatcc gctgtggcaa tttctggctg
     5041 ccatccatcg caatctgcca ggttcgatgg cgtaacgccg ggttgctata gcgttcaatt
     5101 aatcggttag cgtaatcttg caaatcaacg ccctgcactt tcagcgtcgg cgcttgttcc
     5161 tgcaacatca agccatacgc cgcataacga taatgttcat cttccatgca gtcattaatg
     5221 tgctgatatc cagcaagata ccccagatac gccaggaatg aatgactgcc gttgagcatg
     5281 cgcaacttca tctcttcata aggcagcaca tcgctaacca gttcggctcc cgctttttcc
     5341 cattccggac gtccggcaac aaagttatct tctattaccc actggcggaa aggttcacag
     5401 gcaacgcccg caggatcgcg cacaccggta agttgttcga ttttcgccag cgtatcctct
     5461 gtcactgcgg gcacaatacg gtccaccatt gttgatggga aagtcacgtt atctttgtgc
     5521 cagtttttca tcaacggctt gcgcgtagga agtgacaacg tcacgcataa catgaccgtt
     5581 ttctggcatg ttgtcacatg acatgacggt aaatgcggga agtcctgccg ctttacggcg
     5641 agccagcgcc tcaacaatca ctcctgttgc tgttttcggc tggtggggat tttgcacgtc
     5701 ggcagcgacc atcgggtgat cgagcattaa ctgtccggtc gccggagagt ggaaataccc
     5761 tttttcggtg attgtcagag agacaatcgc gatttgcggt tcacacatcg cagccaacac
     5821 ggtttctaag ccatccatct gtacgtgcaa ggctttttta acgacgccaa cgacgcgagc
     5881 cgtccacgca tcggccgaca tttccgcaac ggtataaaga ttatcttgct gctgtaaatc
     5941 ggcaatttgc tgttcgccgc cgattaagtt gacctcataa tatccccagt cactgaaatg
     6001 ttctgtagca agaatatcgg catacacgcc ctgatgcgca cggtgaaatg caccaaagcc
     6061 taaatgaaca attcttgggg ccaggttatt acgatcataa acagggagtg tcgcttttgc
     6121 tgataacaaa ttatttccca taacaattcc ttaaatttaa atatggcaag ctacatgttt
     6181 tgttatatga ataaaaatcc cctctccggt aagagaaggg attgaggatt tacagacttc
     6241 tggaaggttg cgcagctctt acaacacgcg gttgatcttc cgcagcgtct tccagcgcac
     6301 ttaaatcacg gtctttcacc tctggcattt tcagcgcaga gattaaacca atcactgaat
     6361 atgccatgat cataatggcg atcggatacc aggattccgt catggtgcag aaaatacccg
     6421 ccaggatagg accaaaaccg gaagcgataa gaccaccaat ttcttttgaa atagccatcc
     6481 gggtaaagcg gtttttacag ccgaacattt ctgccatggt aatgttttcc agagcaaata
     6541 atcccagcac cgcacagtta tgaatcacaa tcagtgcaac cataatggtg ctcggggcat
     6601 agcttttatc tacgatgata gaaagcattg gccacgccag cacaatcgca gaggtattca
     6661 taataatata cgggatccgg cgaccaattt tatcggataa ccaaccaagg aacggaatgg
     6721 tcataaagcc gagaatcgaa ctgatcatca atgcatctgt tggaattgct ttgttaaaca
     6781 ataacgtctg tactaaatag cctgcaagga aagtctgaat taaaccggag ttacccgcct
     6841 gaccaaaacg cagccctgtt gccagccaga aggatttgct ctggaacatg ctaccagcag
     6901 gtgcaggttt tgctgtcggt tggttgctgt cgttaacctt ctcaaagacc gggctttctt
     6961 tcagattcat acgcaaccag atagcaaaga ccatcacgac aacgctcgcc aggaacggta
     7021 tacgccatcc ccacgccagc agttcctctt tactgagaat gaagaacata aaggcccaga
     7081 ttgccgttgc gcttaaggtt ccgcagttag ttcccatagc cacaaatgag gagataattc
     7141 cgcgcttacc ttttggcgca tattccgcca gcatcgtacc ggcaccggaa atttccgcac
     7201 ctgcacccaa tccctgaata atacgcagcg tcaccagcaa gatgggtgca aaaacaccaa
     7261 tctgtgcata ggtcggtaac actccaatta aggtggtaca gatccccatc atggtgatgg
     7321 taataaagag cactttttta cgccctattc tgtcgcccat tttgccgaaa ataaatgctc
     7381 cgacaatacg cgccacataa cctgcaccat aggttcccat tgccagaatt aacgccattg
     7441 ccgttgatga ttcaggaaaa aatatttcat gaaacactaa cgctgcgccg agcgaatata
     7501 actggaaatc cataaattca agcgcagtgc ccagccatcc cgatactgct gctttcacca
     7561 aatccttagt gcttctttca tgtttttcta ttgtcataat ggttatctct aaaaaagagg
     7621 taagatgcgt actacttact cgccgttatt ggtattattc agaaaaagtg agtaagactt
     7681 tgcagcaatg tttttgatcc tgttcaaata aactaatggc atcagcaaca tgctggaaat
     7741 caaacgtatg ggtaattaat ttttctggtt taattaaccc tttacttaac cagtcgataa
     7801 caaccggaaa tttatttgca tttaagcgtg aagagaaaat agagagttct tttccggtaa
     7861 ttccttgctg aatcacttca gacggttcac tggagaagcc catcaataca atacgtgccg
     7921 ctggagaagc cagcgttacg gcctctttca ggatagaagg atgacaagcc gcatcgataa
     7981 ttaatgtcgg cttgatgcct ttttcagcga aaatctcgcc aagcggtgtc tggctgttat
     8041 taatcgccca gtctgccccg ctctctttcg ctttttccag tcgttcatca atgcgatcgg
     8101 caacaatcac atttttaacg ttatagacgc cttttaatac ctgaacgatc gtcaggccga
     8161 ttggaccggc accataaacc agaacggtat cattttcagt cggttgacca tgaccggtaa
     8221 cgttagccgc aatggtaaaa ggttcgatca tcaccgcata ttgatcggcc actgcttcag
     8281 gaattttcca cgcatttttt gccggaacca cggcatattc actgaaacca ccgtcagcgt
     8341 gcacacctaa tacagcaagt gtcgtacaaa cgttcggctt acctatagag cacggatagc
     8401 aatgcccaca gctgaccacc ggatcgacag caacgcgttc accgactctg gcgctttcca
     8461 caccttcacc caccgcatca atgacgccaa agaattcatg accaatgacg cgcggatatt
     8521 tcgcaaaagg attatgccca cggtaaatat ggctatctga accacaaatt ccggcaagtt
     8581 tcacttttac tcgtacttca cccgctgacg gggtgggtat ttcacgttcg ataatcgaca
     8641 gttgattcgg tttttcaatt aaaatgcttt tcattacctt actccttacc agttccacag
     8701 cgtgccatct tccagacgtg cgactggcag ataagcaggt tcatagggat atttcgccgc
     8761 cagcttttca tcgaattcga tgccaagacc cggtttgtct cccggatgca tatagccgtt
     8821 atcgaaagtc cagttgtgcg ggaagacttc gagcatttgt tcggaataac ccatgtactc
     8881 ctggacaccg aaattgggga cccacagatc aaagtgcagc gccgcagcca tgcagactgg
     8941 tgacaaatcg gaaggaccgt gtgagccagt acgtacctga tacagcgaag caaaatcggc
     9001 aatccggcgc ataccggtaa ttccgcctgc atgggtcagc gtggtgcgga tataatcgat
     9061 gagttgctct tcaatcagtt gtttgcagtc ccagatgctg ttgaagactt cacccactgc
     9121 gatgggtgtg acggtatgtt ggcgaatgag acggaagcat tcctggtttt ccgcaggcgt
     9181 cgggtcttcc atccagaaca tgcgataatc ttcaatgctt ttaccaaagc gcgccgcttc
     9241 aataggcgtt aagcgatggt gcatgtcatg cagcaaatgt tcattaaaac caaacttgtt
     9301 acgtaccgcg tcaaacaatt tcggcatgaa atcgaggtat ttctccgtcg accacagctg
     9361 ctcttccggc cactgtcctt tggttgcggg ttcataagcc agacctttac ctttcgacat
     9421 gccgtaggtg gttttcatac cagggattcc gcactgcacg cggatggctt tgaatcccag
     9481 ctcctgatga cgggcataat catccagagc ttcatcaata ctgtgaccgg tggtatggca
     9541 ataaaccatc accccttcac gagacgcgcc gccgagtaac tggtaaagcg gcatgttggc
     9601 agctttggct ttaatatccc acagcgccat atcaaccgct gaaatggccg acatcgtaac
     9661 cggaccgcga cgccagtaag cacctttata gaaaaactgc cagatatctt cgatacggtg
     9721 cgcatcgcga ccaataagct gcggacaaag gtgatcctgc aaataagagg ccacggaaag
     9781 ctcacgtcca ttgagggtgg catccccaag gcccgtaata ccgtcctcag tggtgatttt
     9841 taatgtgacg aaattacgcc ccggacaggt aacaaaaact tcagccttta cgatcttcat
     9901 gttcgattcc ttgcatcgct tgtcgtgatg catgaaatct acgcaaccga gctactacca
     9961 tacaagtata aagatcgaaa aaagccggag tgatcacaaa aaaaggcgta tatttgcgct
    10021 gtgaatggtt gacaaaagat gaaatagaat accttttgtc agctgacact tcctcttatc
    10081 ttattgataa aatggattta tgttcctacg tgcgccccca gcccgcaaca atgatcaaca
    10141 tgccgcaaag cgcaatcaac gcacccgtcc agtcataaag agtcagtttc acgccatcca
    10201 caacgcgcag ccacatcaac gccgtgcaga cataaacgcc accataagcc gcgtaaacac
    10261 gcccactcgc cgctggatgc aacgttaaca accagacaaa cagcgccagt gaaatccccg
    10321 ccggaagcaa cagccagata ctggcgtttc gttttaacca caaccagggc agaaagcatc
    10381 caataatttc acacagcgca gtagcaaaaa atagtaacgt tgttttaatc atctttgtca
    10441 cttattgaca tcatgtatag ttatagggcg acataatatc atcaatataa acaccctcct
    10501 ggtacgtttt gcgttcgcag tggatggtgt agaatcacct ttaatcattc atacagggaa
    10561 tgaattatga aaatcactct cagcaaacga atcggcctgc tcgcttttct gctgccttgc
    10621 gcactggcac tgagcacaac tgttcatgcc gaaactaaca aactggtgat tgagtctggc
    10681 gacagtgcac aaagccgcca gcacgccgct atggaaaaag agcaatggaa tgacacgcgc
    10741 aatctgcgtc agaaagtgaa taaacgcact gaaaaagagt gggataaagc cgacgccgct
    10801 tttgataacc gcgataaatg tgagcaaagc gccaacatca atgcctactg ggagccaaat
    10861 actttgcgct gcctggaccg tcgaactggc cgcgttatta ccccctaacc tgttattgat
    10921 ttaaggaatg taaggacacg ttatgccaag cgcccacagt gttaagctac gcccgctgga
    10981 gcgtgaagat ttacgctatg tacatcaact cgacaataac gccagtgtga tgcgttactg
    11041 gtttgaggaa ccctacgaag cctttgttga actctctgat ctgtatgata agcatattca
    11101 cgatcagagc gaacgacgct ttgtggtgga atgtgacggc gaaaaagccg gtctggtgga
    11161 gctggtggaa attaaccatg tacatcgccg ggcagaattt cagataatta tctccccgga
    11221 gtatcagggg aaaggtctgg caacccgtgc cgccaaatta gcaatggact atggcttcac
    11281 cgttctcaat ctctataagc tgtatctgat cgtcgataaa gagaatgaaa aagcgattca
    11341 catttaccgc aagcttggct tttcggttga aggtgaattg atgcacgagt tctttattaa
    11401 tggtcaatat cgtaatgcca ttcgcatgtg tatattccag catcagtatc tggcagagca
    11461 caaaacaccg ggtcagactc tcctgaagcc gaccgcacaa tagcattaat aataatcgat
    11521 cgtatttttg atggtgtaaa cccgttcgac ggcgggtttt actccttcat caacaatgat
    11581 cagctgacag tccaccggat tggcgtgact gtcatattca cagctctgtt ttacattacc
    11641 aactcgttga ttattcagta aagtaaccgc tgtgtaatct aattttttga tcgggtccgt
    11701 tgatggcgtg gcgctgacag ataatgtttt gtcgttactt ttcgtggttt tacccagtgg
    11761 ataaccctga tcatcatagc gatattccat ctgcatttgt ttgctgctgg ctttaatcac
    11821 gaagccatta tcatcggttt cccaactcac cccggcagaa ggtaattctg ctagctggca
    11881 ttttccctgt aaacgtactc tcttctccag cgtctcggca tcacggtaat aattggcgtc
    11941 cagtaccaga gcgaccacgg tattattttc cagatccagt aattcgagtg aatcaaaaca
    12001 gccttcttcc gacaaagtcc cagaaacacg tttcgtcact tcaccttgct catccattaa
    12061 tgtctgagtg aaatctttta ccggaccacg cagcggatca aaatcgaatt cattagagaa
    12121 actggccatc tcaggggtaa atgaaagtgt tacctctgtg cggtcacatc ctgtgaggaa
    12181 tatcgcgagt aagcatggta gtaatttgta tttcacaaca gtcaccagag agtagagatg
    12241 attctcaatc atagtagcaa atacagtact ttacacgtta aatgctatgc ttaaagaagt
    12301 tatcttcgcg taaggagctt atgatgaaac tctcaacctg ctgtgccgcg cttctgctcg
    12361 ccctcgcctc acccgcggta ctcgccgcgc cgggatcctg tgagcgcata cagagcgata
    12421 tatcacagcg cattatcaat aatggcgtac ccgaaagcag cttcacatta agtattgtac
    12481 ccaatgacca ggttgatcag cctgattccc aggtcgtcgg gcattgtgct aatgatacgc
    12541 ataaaattct ctacacccgc accaccagcg gtaacgtctc tgctcccgcg cagtctaccc
    12601 aggatggcgc gcctgccgaa ccgcagtaat acattatcga ctgaacgccg gatacgacac
    12661 atccggcgat ttgaacatac aacataatcc caccttatta ctcataccct tctattgata
    12721 tggattaata attcttaacc caaaatgggt agactccctc tattgttagc gcgctaaata
    12781 ttcaatatat aaacttttat ataacgataa agaacaggga gtgagttatg tccaaaaatg
    12841 aacgaatggt gggcatcagc cgcagaacac tcgttaaatc taccgcgata ggttctctgg
    12901 cgctggctgc aggcggtttt tctttgccgt ttaccctgcg cagtgcagca gcagcggtac
    12961 aacaggcccg cgaaaaagtg gtctggggtg cctgttccgt caactgtggt agccgctgtg
    13021 cacttcgtct acatgttaaa gataatgaag tgacctgggt ggaaactgac aataccggca
    13081 gcgatgagta cggcaaccat caggtacgcg cctgtttgcg cggtcgctcc atccgccggc
    13141 gtattaatca tcccgatcgc ttgaattacc caatgaaacg cgtgggtaca cgcggtgaag
    13201 gcaaattcga acggattagc tgggatgaag ccctggatac tatcgccagt agcctgaaaa
    13261 aaaccgtcga acaatatggc aatgaggctg tatatattca gtactcttcg gggattgttg
    13321 gcggcaatat gacccgctct tcgccatcag cctcggcggt caaacgcctg atgaactgct
    13381 acggcggttc actcaatcag tatggctcct acagcaccgc gcaaatttcc tgtgccatgc
    13441 cctacaccta cggcagtaat gatggcaaca gcaccacgga tattgaaaac agcaagctgg
    13501 tcgtgatgtt tggtaacaac ccggcagaaa cccgcatgag cggcggtggc atcacttatc
    13561 ttcttgaaaa agcgcgcgag aaatcgaacg ccaaaatgat tgttatcgat ccgcgatata
    13621 ccgatacggc tgctggtcgt gaagatgaat ggctccctat tcgcccgggc accgatgccg
    13681 cgctggttgc gggtattgcc tgggtattga ttaacgaaaa tctcgttgat caaccttttc
    13741 tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca cccaaaaata
    13801 gtcactataa agcctatatt cttggtgaag gtgatgataa cacagcgaaa acgccgcagt
    13861 gggcttcgca aattaccggt atcccggtag accgtatcat caaactggcg cgtgaaattg
    13921 gcacagcaaa acccgcttat atctgccagg gctgggggcc gcaacgccag gcaaacggcg
    13981 aactgactgc acgcgctatt gctatgttac ctattttgac gggcaatgtc ggcatcagcg
    14041 gcggaaatag tggcgcgcgt gaatcgacct ataccattac catagaacgc ctgccggtgc
    14101 tggataatcc ggtcaaaacg tcaatctcct gcttcagctg gacagatgct atcgatcatg
    14161 gtccgcaaat gacggcaatc cgcgacggcg tccgcggcaa agataaactg gatgtgccca
    14221 ttaagttcat ctggaactac gcgggaaata ccctcgttaa tcagcattct gacatcaaca
    14281 aaacgcatga aattctgcag gacgaatcga aatgcgaaat gattgtggtc atcgaaaact
    14341 ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc agacctgatg accgttgagc
    14401 aggaagatat tattcctaac gactacgccg gtaacatggg atatctcatt ttcctccagc
    14461 ctgtcaccag cgaaaaattc gaacgcaaac cgatttactg gatcctgagt gaagtcgcga
    14521 aacgtcttgg acctgacgtc tatcaaaagt tcacagaagg tcgcacgcag gaacaatggt
    14581 tacaacatct gtacgccaaa atgcttgcca aagatccggc gttaccgtct tacgacgaac
    14641 tgaaaaaaat gggtatctat aagcgtaaag atcccaatgg ccattttgtc gcctacaaag
    14701 catttcgtga cgaccccgag gcaaatccac ttaaaacgcc ttccggtaag attgaaattt
    14761 attccagcag gctggcggaa attgcccgta cctgggaact ggaaaaagat gaagtgataa
    14821 acccattgcc agtttatgcc tcaacctttg aaggctggaa ctcccctgaa cgcagaacct
    14881 tcccactgca actgtttggt ttccattaca aatcccgtac tcactcgacc tacggcaata
    14941 ttgatctcct gaaggctgcc tgccgtcagg aggtgtggat caaccctata gatgcgcaga
    15001 aacgtgggat tgccaacggc gatatggtgc gggtgtttaa ccatcgtggc gaagttcggc
    15061 taccagccaa agtaacacca cgtattctcc ctggagttag cgctatgggc cagggagcct
    15121 ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg cggctgtgtg aatacgctaa
    15181 ccactctgcg cccttcacca ctggcgaagg gaaacccgca gcacactaat ctggtcgaga
    15241 tcgaaaaaat ataacccacg acaaccataa attctggcat gacattttgt ttgaaaagca
    15301 ataagtgagt aatgatgaaa atccatacca cagaggcgct catgaaggct gaaatcagcc
    15361 gtagaagtct gatgaaaacc tccgcacttg gcagtcttgc gctggcaagc agtgctttca
    15421 ctctgccatt ttcccaaatg gtccgggcgg cagaggctcc ggtagaagag aaagcggtct
    15481 ggagttcctg caccgttaac tgcgggagcc gctgtctgtt acgtttgcat gtgaaagatg
    15541 acaccgtgta ctgggtggag tctgatacga caggtgacga cgtctacggt aaccatcagg
    15601 ttcgagcgtg tttacgcggg cgctctatcc gccgacggat gaatcatcct gataggttga
    15661 aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa atttgaacgg ataagttggg
    15721 acgaagccct ggataccatc agtgataatc ttcggcggat cctgaaagat tacggcaatg
    15781 aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg aaacatcacc aactcaaacg
    15841 tcccgtaccg tctgatgaac tcttgcggtg gttttctcag tcgctatggc agctacagta
    15901 ccgcacagat cagtgccgca atgagttata tgttcggtgc caatgatggc aacagcccgg
    15961 atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa taacccggca gaaacgcaga
    16021 tgagcggcgg tggtgtcact tactacgtcg agcaagcccg cgaacgttca aacgcacgca
    16081 tgatcgtcat cgatccacgt tataacgaca ctgctgccgg gcgtgaagat gaatggctgc
    16141 ccattcgccc tggcaccgat ggcgcactgg cctgtgcgat tgcctgggta ctgattactg
    16201 aaaacatggt cgatcagcca tttctcgaca aatattgtgt tggttacgat gaaaaaacgc
    16261 tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta tattctgggc gaagggcctg
    16321 acggcatagc taaaacgccg gaatgggcag caaaaatcac cagcatcccg gcagaaaaaa
    16381 ttatccagtt ggcacgagag atcggttcag caaaacctgc ttatatttgt cagggttggg
    16441 ggccacaacg acattccaac ggcgagcaaa cgtcccgcgc tattgccatg ctttccgttc
    16501 tcaccggcaa cgtcggcata aacggcggca actcaggcgt acgcgaaggt agctgggatc
    16561 tgggggtaga atggttcccg atgcttgaga atcctgttaa aacgcagatt tccgtcttta
    16621 catggacaga tgccatcgac catggtacgg aaatgaccgc gacccgtgat ggtgttcgtg
    16681 gaaaagaaaa actggatgtc cccatcaagt ttttatggtg ctacgccagt aacacattga
    16741 tcaatcaaca tggcgacatc aatcacaccc atgaggtgct tcaggatgac agcaagtgcg
    16801 agatgattgt tggcattgac cacttcatga cggcctcggc taagtattgc gatatcctgt
    16861 tgcccgacct gatgccaaca gagcaagaag accttatctc tcatgaatct gcagggaata
    16921 tgggctatgt gatcctcgcc caacccgcaa cctcaccaaa atttgaacgc aaacccatct
    16981 actggatgct gagtgaagtc gccaaacgct taggaccaga cgtttatcaa acctttactg
    17041 aaggtcgcag tcagcatgaa tggatcaaat atctccatgc gaaaacgaag gaacgtaacc
    17101 ctgagatgcc cgactacgag gagatgaaaa cgaccgggat ctttaagaaa aaatgcccgg
    17161 aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc acaggcaaac ccgttgaaaa
    17221 caccttcggg gaaaatcgaa atttattctg aacgactggc gaagattgca gatacctggg
    17281 aattgaaaaa agatgaaatt attcatcccc ttcctgcgta taccccaggt tttgatggct
    17341 gggacgatcc cctgcggaaa acctatccac tgcagttaac gggcttccac tacaaagcgc
    17401 gtacccactc cagctacggc aatattgatg tgttacagca ggcctgccca caagaggtgt
    17461 ggatcaaccc cattgatgct caggcacgcg gtatccgtca tggcgatacc gtgcgggtat
    17521 ttaacaataa tggagaaatg ctgattgccg caaaagtgac tccgcgtatt ctgcctggcg
    17581 ttaccgccat cgggcaaggt gcgtggctta aagcggatat gtttggtgac cgggtcgatc
    17641 acggcggcag tatcaatatt ctgacctctc accgcccttc accgctggca aagggaaacc
    17701 cgtcgcacag caatcttgtc cagatcgaaa aggtttaagg agtagcccat gaccacacaa
    17761 tatggatttt ttatcgattc cagccgctgt actggctgta aaacctgcga actggcgtgc
    17821 aaagatttta aagatcttgg cccggaagtc agtttccgcc gtatttatga atatgctggc
    17881 ggcgactggc aggaagataa tggcatctgg caccagaacg tgttcgctta ttatctctcc
    17941 atctcctgta accattgcga tgaccccgca tgtacaaaag tctgccccag cggcgcaatg
    18001 cataagcgtg aagatggctt tgtggtggtt gatgaagatg tttgtatcgg ctgccgctac
    18061 tgccacatgg cttgtccgta cggcgcacca cagtacaatg ctgaaaaagg gcacatgacg
    18121 aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac aacccatatg cgtcgaatcc
    18181 tgcccgctgc gtgcacttga gttcggtcca attgaagagc tgcgccagaa gcacggcact
    18241 ctggctgccg tcgcgccact gccgcgcgcg catttcacaa aacccaatat cgttatcaaa
    18301 cctaacgcca acagccgccc gaccggcgat accactggtt atctggctaa tccggaggaa
    18361 gtgtaaaatg ggaaatggat ggcatgagtg gccactggtt atctttaccg tattgggtca
    18421 atgcgtagta ggcgcgctga ttgttagcgg aattggctgg ttcgccgcca aaaatgacgc
    18481 agaccgtcag cgtattgtcc gtggcatgtt tttcctctgg ctgttgatgg gcatcgggtt
    18541 tattgcctct gtcatgcacc tgggatcgcc actgcgagcg tttaactcgc tgaatcgaat
    18601 cggagcttct ggtctgagta acgagattgc tgccggttcc atattttttg ccgttggcgg
    18661 actatggtgg ctggtggcgg tcatcggtaa aatgccgcaa gcgtcgggga aactctggtt
    18721 acttgtcagc atggcgctcg gcgtcatttt cgtctggatg atgacctgcg tgtatcaaat
    18781 cgacaccgtg ccaacctggc ataacggtta cactacgctg gctttcttcc tgacggtatt
    18841 gttgagcggt ccaatacttg ctgccgcaat tctgcgggca gctcgcgtta catttaatac
    18901 cacaccattt gccatcatta gcgttttggc gctgatcgct tgtgccgggg tgattgtgct
    18961 gcaaggtttg tctctggcgt gtattcactc atccgtacaa caagccagcg cactggtacc
    19021 agattacgcc tccttacagg tctggcgtgt ggtattgtta tgcgcaggtc taggatgctg
    19081 ggtttgtccg ctcattcgtc gccgtgaacc tcacgttgcc ggacttattc tggggttaat
    19141 cctcattctt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacggt
    19201 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
    19261 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
    19321 gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag
    19381 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
    19441 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
    19501 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
    19561 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
    19621 gaagcaaaac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
    19681 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc
    19741 aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
    19801 tgaactggct cgcttaacgc tggcgcaatg gcaatcacaa ctgttgattc ctgtcgcggt
    19861 taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat
    19921 ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg
    19981 cggctggcct cttttgccgc aaaatagtcg tccgtgtttc attgcctatt tctgctcatg
    20041 catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca
    20101 gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc atcatgcgat gctgctactg
    20161 gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc
    20221 ccctggcgac ggctgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg
    20281 ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg
    20341 caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg
    20401 gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt
    20461 gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt atggttcgcc
    20521 tgtggggccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt
    20581 atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc
    20641 gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac
    20701 gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg
    20761 ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt
    20821 gtgagtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg
    20881 ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta
    20941 accgccccac cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg
    21001 ctggcgagta gcggttccgg cgcacccggt ggggtcttta caccgacgct atttatcggt
    21061 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
    21121 attacacttt tactcggatt gaccgggatg gcgacactgt tggcggcgac cacgcacgcg
    21181 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
    21241 ttattgattg cctgcgtaat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
    21301 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt cacgctgttc
    21361 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
    21421 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
    21481 gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc
    21541 ttcctgaata ccgacaacca tcaacaccgg cagttgttcc tgcactaccc attcagagag
    21601 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
    21661 gactttttcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
    21721 gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggcag
    21781 ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt
    21841 ctctttgctc cccttcgcta cgggtttata tcccgcaacc gtttttccct gggaggctaa
    21901 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
    21961 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aaccaggaaa
    22021 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
    22081 ttggtagaac agttaaaaaa tgttaaccct gcaacagacg aatcaacaaa gaaccgttat
    22141 acatcgcgtc ttttaccagc gcagcccctg ccatcgtacc ctggttagaa aactgagtac
    22201 tctcaacgct gatgtgctga ctatacgcag gaagggcctg ctgacggatg ctgtctgaga
    22261 tgaccgggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
    22321 ggttaaataa attcaccatg atggcaagaa tgcgccctac atgcgcgccc accccggtaa
    22381 tgatgtcttt tgccagcaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
    22441 acggttgtcc atgtaacatc gagctcatgg attgattaag acgcagctgt gccagctcaa
    22501 gaatactgtc cacactggcg atggtttcga ggcagccgtg attcccgcaa taacagcgtt
    22561 tcccatacgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
    22621 gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca
    22681 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctaatat
    22741 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
    22801 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
    22861 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
    22921 actgatcgat atgggaaatg atacgatcca gcaatggcga gtcatctttt aacgccagtt
    22981 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctccc
    23041 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agccccaccg
    23101 ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccagg tgtgcttcga
    23161 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
    23221 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
    23281 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
    23341 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
    23401 tttacttagg catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca
    23461 cctgagagca ttcagcagat gaatacgaaa gtttcgcgca gctggacttt gttcatgatg
    23521 tttcggccag accaaccaca tttcagaaac cgcatcctct tctgcaatcg gcacccagcg
    23581 catttcgttg agctgaacac gtttaaatga cgcaggcaaa attgaaacac ccagaccggc
    23641 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
    23701 atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc
    23761 aaaaaagacg aagggttctt tcgccagttc agccagcgtt acattcgggt tatttgccag
    23821 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
    23881 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
    23941 agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacatcgg gataatcacg
    24001 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
    24061 aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
    24121 caccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
    24181 tacactgcga ttggttcgtg ccagcagtcg ggcaccaatt tgttgctcca gcgcctgaat
    24241 ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag
    24301 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
    24361 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
    24421 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
    24481 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
    24541 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
    24601 atttgcactt ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
    24661 aacgcccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
    24721 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
    24781 actgttggcc tccatttgta cgttactttc gacaatgatg accagctggc acggcatttt
    24841 gattatgcgc gccttgattg ggctttcgtt aagtggcgtg gcagctgttg gcatgactta
    24901 tcttagcgag gaaatccacc ccagtttcgt ggccttttcg atggggttgt atatcagcgg
    24961 caactcaatt ggcggcatga gcggacgctt aattagtggt gtcttcacgg actttttcaa
    25021 ctggcgaatt gctctggcgg caatcggttg tttcgcgctg gcctcggcgt tgatgttctg
    25081 gaaaatcctt ccggaatcac gccattttcg cccgacttcg ctgcgcccta agacgttatt
    25141 tatcaacttt cgtctgcact ggcgtgaccg gggattaccg ttattgttcg cagaaggctt
    25201 tttgctgatg gggtcgttcg tcacgctgtt taattacatc ggctatcggt tgatgctttc
    25261 cccctggcat gtcagtcagg ccgtggttgg cttattatcg ctggcttatt tgaccggtac
    25321 atggagctca cccaaagccg gaaccatgac cacccgctac gggcgcggtc cggtgatgtt
    25381 gttttcgacg ggggttatgc tgtttggttt actgatgacc ttattcagct cgctgtggct
    25441 gatctttgcc ggaatgttac tcttctcagc aggattcttc gcagcccact cagttgccag
    25501 cagctggatt ggcccccgcg caaaacgcgc taaaggccag gcctcctcgc tgtatctgtt
    25561 cagttactat ctggggtcga gtattgccgg gacgctgggt ggtgttttct ggcataacta
    25621 tggctggaac ggcgtcggcg catttattgc tctgatgctg gtcattgctc tgctggtcgg
    25681 gacgcgtttg catcgtcgtc tgcacgcctg aaaaataagc ccggactgcg gtaactaccc
    25741 gtccggactt attgcttgct caaaccaacg ttaatagcca tcctaaaata gacgaagcgc
    25801 cagccaattc ccgcagcgcg tctagcgtca tcaggattat aagtacccaa ataaacggat
    25861 tcattttgct gtgtgtcatt tattactgat gcgcagttat tctaccgctt tgtaagtagt
    25921 aaaatagtta acccgatcaa gactactatt attggtagct aaatttccct taagtcacaa
    25981 tacgttatta tcaatgctgt aatttattca gcgtttgtac atatcgttac acgctgaaac
    26041 caaccactca cggaagtctg ccattcccag ggatatagtt atttcaacgg ccccgcagtg
    26101 gggttaaatg aaaaaacaaa ttgagggtat gacaatgaaa aaagtattag ctctggttgt
    26161 tgccgctgct atgggtctgt cttctgccgc ctttgctgca gagactgcga ccacacctgc
    26221 tccgactgcg acgaccacca aagcagcgcc ggcgaaaacg acacatcata aaaaacagca
    26281 taaagcagca cctgcccaga aagcgcaggc ggctaaaaag catcataaaa atacgaaagc
    26341 tgaacagaaa gcccctgaac aaaaagcgca ggcagcgaag aaacacgccg ggaaacacag
    26401 ccatcagcaa ccggcaaaac ctgctgcaca acccgcagcg taagtttaca acagtaatgc
    26461 tggcgcgccc cctcgcgcct gaaaattacg gtgctaagcg ggtaacgttt agcaccgcct
    26521 ttagcggagg gtagtatggt gggccgttat cgctttgagt tcattcttat catccttatt
    26581 ttatgcgcac tgattgccgc ccgtttttat ctttcctgac tgtagttatc tgattttact
    26641 cccactttca tcccgtcccg tctatagtat ttatgagggt ttgcttttaa taatcataat
    26701 tacccaccag agtgtgatat gcgtacaacc attgctgtag tgttgggtgc aattagtttg
    26761 acgtctgctt ttgtgtttgc agataaacca gacgttgcca aatcggcaaa cgatgaggtc
    26821 agcaccctgt tttttggtca tgatgatcgt gtgccagtga atgacacgac ccaatcaccg
    26881 tgggatgcgg ttgggcaact ggaaacggcc agcggcaatt tatgtacggc gacgctgatt
    26941 gcacccaatc tggcattaac ggcaggacac tgtttattga cgccgcctaa gggtaaagcg
    27001 gataaggcgg tagcgctgcg ttttgtgtca aataaaggtc tttggcgcta tgacatccac
    27061 gacatagaag gccgcgttga tccgacactg ggaaagcggt taaaagcaga tggggatggt
    27121 tggattgttc ctcccgcagc tgcaccatgg gacttcggct tgattgtgct gcgtaatccc
    27181 ccttctggca ttacaccgtt gccgttattt gagggagata aagccgcgct tactgccgca
    27241 ttaaaatcgg ctggtcgtaa agtgactcag gcgggatatc ctgaagatca tctcgatacg
    27301 ttatacagtc atcaaaactg tgaagtgact ggctgggcgc aaacgtcggt gatgtcacat
    27361 cagtgcgata ccttgccggg tgacagcggt tcgcctctga tgttgcatac cgatgacggc
    27421 tggcaattaa ttggggtgca aagttcggct cctgccgcga aagatcgctg gcgcgccgat
    27481 aaccgggcca tttctgttac cggttttcgc gacaagctgg atcaactgtc gcaaaaataa
    27541 tgttcaaacg ctgcccgact gtgcgggcag cgtgttcatc aggcaagttt caccatgatc
    27601 attccagcca acagcaagac caggccaatc cagcctttac gatttaaccg ttgaccaaac
    27661 aagatccaac ctgcggctaa cgtggcggca ataccaaacc cgccccacaa tgcataagcg
    27721 acagacaagt cgatcccttt aacggcctga gaaagcgcac taaaggcagc cagcaccgcc
    27781 gccagggaga gcaagccaaa tattttgcga cgaaagccgt cagaaaattt caaaaagacg
    27841 ttagcaacga tttccagcac gattgccaat gccagccagg cggcgtgaac ccattcaaac
    27901 tgcgccatgg ttcacctcca gttcaggttt acgcgcttta cgggtacctg attttatcaa
    27961 cacaatcccg gcgaccaggg tggttaaccc ggcaattttc atcagcgata aactttcgtc
    28021 gaataacaaa acgctaaaca aggtaataaa taaaataccg ataccttccc acagcgcata
    28081 agctacgcct aaggcgattt ttttaacggc gaaagagaga aatatatacg acagagaaat
    28141 catcaccagc attaaaataa agccgccatt tccctcactg acgctcgccc atttcatgga
    28201 cagcgtaccg gtaatttctg tagcaatagc cagacctaat aaaatccaat aaatatacat
    28261 tgtccttctc ctgcaagaga attattttaa ttttcgctta attcagcgaa accaaagtaa
    28321 agtggtcaag ccccgcgcat agggcaagct taggcagaag aaaggactaa agcgcgttgc
    28381 gccagtgctg ctcacctacg agcaagatag tggatgaggt acgaagatgg gtaaatgtag
    28441 aaaataacgt cctgaacaaa ttgtccataa tattacaatt atccgcagtg ttgcttctcg
    28501 tcatcgcgga tgataattgt cctcggtagt tgaacacgcc tgatttgtat catagcttaa
    28561 gaattaactc aaaatatttt cacttcttta cctgagcggt ttgattttcg ttatgatgac
    28621 ggagcgaaaa agacattatt attagcaaag gaagaaaaaa cggggacaag catggcaaag
    28681 ccgatcatca cgctcaatgg cctaaaaatc gtcattatgt tgggaatgct ggtcattatt
    28741 ctctgcggta tccgttttgc cgccgagatc atcgtgccgt ttattctcgc attatttatt
    28801 gctgttattc ttaacccgct ggtgcaacac atggtccgct ggcgagtgcc gcgtgtactg
    28861 gcggtgtcga ttttgatgac catcatcgtg atggcgatgg tgttgctatt agcttatctg
    28921 ggttccgcgc tcaacgagtt gacgcggacg ttaccgcaat atcgcaactc tattatgacg
    28981 ccgctgcaag ctcttgagcc gttgttgcaa cgcgtaggga ttgacgtctc agttgaccag
    29041 ctggcgcatt acattgatcc gaacgcggcg atgacgttgc tcaccaactt attgacgcag
    29101 ttatctaatg ccatgtcatc aatattttta ttgctgctga cggtgctgtt tatgctgctc
    29161 gaagtgccac aattgcccgg aaaatttcag caaatgatgg cgcgtccggt tgaagggatg
    29221 gcggcgattc aacgtgcaat tgacagtgtt tctcattatc tggtgctgaa aacagccatc
    29281 agcatcatca ccggcctggt cgcctgggcg atgctcgccg cactcgatgt tcgcttcgct
    29341 tttgtctggg gattgctggc ctttgcgctt aattacatcc ctaatattgg ttcagttctc
    29401 gcggcaatcc cccctatcgc tcaggtactg gtgtttaatg gcttctacga agcgttgctg
    29461 gtgctggcgg gatatctgct gattaatctg gtcttcggca atattctgga gccgcgcatc
    29521 atgggacgtg ggctggggct ttccacattg gtggtatttt tgtcgttgat tttttgggga
    29581 tggttgttag gaccggtggg tatgctgctt tccgtgccgt taacaattat tgtcaaaatt
    29641 gcgcttgaac aaacagcggg aggtcaaagc atcgccgttc tgttaagcga tctcaataaa
    29701 gagtgacggc ctccgcagag gccgtcaggg ttacagagct ttcaggattg catccacgct
    29761 ggctttggcg tcaccaaaca gcatgtgggt gttttccttg aagaacagcg ggttttgcac
    29821 accagcatag ccagtgttca tcgaacgttt aaagacaatc acgttctgcg ctttccacac
    29881 ttccagcaca ggcataccag caatcggact cttcggatca tcctgcgccg ccgggttaac
    29941 cgtatcgtta gcaccaatca ccagtacggt atcggtatca gcaaagtcat cgttgatctc
    30001 gtccatttcc agcacgatgt catacggtac ttttgcttca gccagcaata cgttcatatg
    30061 tccaggcaaa cgccccgcaa ccgggtggat accgaaacgc acgttgatac cacgagcgcg
    30121 cagtttctcg gtaatttcag cgacaggata ttgcgcctgc gcgactgcca tgccgtaccc
    30181 cggagtaatg atcactgaat gggagttttt cagcagttcc gctgtctctt ctgcggtgat
    30241 ttcgcggtgc tcacccactt cctgatcatc gccagtagaa gagccgtcgg tgccgaaacc
    30301 acccgcaata acgctgataa aggaacggtt catcgcctta cacataatgt aagaaaggat
    30361 agcccccgaa gaaccgacca gcgcaccggt cacaatcagc aggtcgttgc tgagcataaa
    30421 gcccgcagcc gcagccgccc agccggagta cgagttcagc atcgacacca ccactggcat
    30481 atctgcacca ccgatggagg cgactaaatg ccagccgaat accagcgcaa ttgcggtcat
    30541 tatcagcaat gccagcactt gcaggccgac gctgtccgtg cgaacaaata caatcagcag
    30601 caggaaggaa acgaccagag ccgccaggtt cattttgtga cggtttggca gcatcaatgg
    30661 tttagacgaa atcttgccac acagtttgcc gaacgccacc accgaacccg tgaacgttac
    30721 cgccccgatg aagataccga ggaacacttc cgtcaggtga atattgacca gaatcggtgc
    30781 cattcccgcg tcatgatgca gatagctgtt aaagccaacc agcactgccg ccagacccac
    30841 gaagctatgc aggatcgcca ccagttctgg catttcggtc atttcaactt tcttcgccag
    30901 acggatacca attgccccac caatgaccat cgccagcaag atccagccaa cattacccgt
    30961 atccggtcca aaaatggttg cgattaacgc aatcgccatc ccggcgatac cgaagttgtt
    31021 accctggcga gacgtttcat gtttcgaaag accggccaga ctgaagataa acaggatcgc
    31081 ggcaacaatg tatgcagctg taactaatcc tccagacata tgttacccct taatttttgc
    31141 ggaacatttt cagcatgcgc tgagtcacgg tgaagccacc gaaaatatta atgctggcta
    31201 taagcaccgc gataaaacta aggaagctaa cccagccgcc ctggccaatc tgcaacagtg
    31261 ctccgacaac aataatccct gaaatcgcgt tggtgaccga catcaacggt gtatgcagcg
    31321 cgtgcgatac attccacacc acgtaataac cgacaacgca ggccagcgcg aaaacggtga
    31381 agtgcccaag aaactctttc ggcgcaacgc ttgccagcca accgaaaagg atgattgcca
    31441 gcgccatcaa cgcgtattta cgccacggtg agcaggcaca tttttcctca gttttcactt
    31501 ccggtgccgc tttttgtgcc gcctgcggct gagctgatac ctgaatcggc ggtgccggcc
    31561 aggtaatttc gcccgcacgg atcacggtca cgccgcgaat caccacatca tcaaaatcaa
    31621 cagtgatatt gccgtctttc tctttgcaca acagtttcag cagattaacg aggttagtac
    31681 cgtaaagctg cgaggattgc gtcggcagac ggcccggaag atcggtataa ccaatcactt
    31741 tgacaccatt ttccgtagtg aagatttcac ccggcacggt gtattcacag ttgccaccgt
    31801 tttgggctgc caggtcgaca atcacactgc ccgccttcat ggagtcaacc atttcacggg
    31861 taattagctt cggcgctggt ttgcctggaa taagcgcggt ggtgacaatg atatcgacct
    31921 cttttgcctg ggcggcaaag agttccattt ccgctttaat gaacgcgtcc gacatcactt
    31981 tggcatagcc atcgccgctg cccgcttcct ctttaaaatc cagctcgagg aattccgcgc
    32041 ccatactttg aacttgttct ttcacttccg gacgggtgtc gaatgcacgc acaatcgcgc
    32101 cgagactgtt tgctgcgcca atggcggcca gacctgcaac acccgcacca atcaccatca
    32161 cttttgccgg tggcactttc ccggccgcag taatttgccc ggtaaagaag cgcccaaatt
    32221 catgtgccgc ttcaacaatg gcgcgataac cggcgatgtt cgccatcgag cttagtgcgt
    32281 ccagcgattg tgcgcgtgag atacgcggca cagaatccat cgccatcacg gtcacgttac
    32341 gttccgcaag tttttgcatt aattccggat tctgcgcagg ccagataaaa ctcaccagcg
    32401 ttgtcccagg attcagtaac gcaatttcat catctaacgg cgcattgacc ttcagaatga
    32461 tctctgactg ccagacgcta ttcccttcta caatttcagc gcccgcttgc acaaacgctt
    32521 tatcgtcaaa acttgccagt tgacccgcgc cgctctctac cgcgacggta aaacccagtt
    32581 tcagcagctg ttccactgtt tttggcgttg ctgcaacacg ggtttcattg gttaaccgtt
    32641 ctcttggtat gccaattcgc atgatattcc cttccatcgg ttttattgat gatggtttgc
    32701 ctgtgtcagg agccacacaa gccgctcatg tacgagctaa atgttactcc gttaaaataa
    32761 attagtaaca aacgccctat aacgtactga aaattatgcc tgtgatctag cgccaaaaat
    32821 cagtatttcg gcgtgaactc gcaaaatatt aacgattcag ccgtgatagt gggataaaca
    32881 ccttagaacg ccggataaag actgataatt gtcttcgacg gtcgggtaaa acgagacatc
    32941 gccccggcac gattcactac ttaacattaa attaacttat acaattcagt tgcttcagta
    33001 gtaatgatgc tgatacggct gttttttaag catagacggt catttgagca ggattaaaat
    33061 tggcttaagg aatgtgatat gaaaaatgac gcagacagtt acaccgttta aatgcaataa
    33121 tcagccacgt ttctcgttaa taacaatacc agtacctggt ttgcgcaagg cgaaggatta
    33181 tttttatgaa gcttaagaac accctcctgg cgtcggcact gctttctgct acggcattct
    33241 ccgttaacgc agcaacagaa ctgacaccgg agcaagcggc agcggttaaa ccttttgacc
    33301 gtgtagtggt taccggtcgt tttaatgcta ttggcgaagc ggtgaaagcc gtttctcgtc
    33361 gcgcagataa agaaggtgcc gcctcttttt atgttgtcga cacttctgat tttggtaaca
    33421 gcggtaactg gcgtgtggtc gctgacctct ataaagccga tgctgaaaaa gcagaagaaa
    33481 caagtaatcg cgtaattaac ggtgttgtcg aactgccgaa agatcaggct gttctgattg
    33541 aaccgtttga cacggtcacc gtccagggct tctatcgtag ccagccagaa gtcaatgatg
    33601 ccatcaccaa agcggcaaaa gcgaaaggtg cctactcttt ctacatcgtt cgtcaaatcg
    33661 atgccaacca gggcggcaac cagcgtatta ctgcattcat ctataaaaaa gatgctaaga
    33721 aacgtatcgt ccagagcccg gatgtgatcc cggcagattc cgaagcagga cgtgcagctc
    33781 tggctgccgg tggcgaagcc gcgaagaaag ttgagatccc aggtgttgcg actaccgcat
    33841 caccaagttc tgaagtcggt cgtttctttg aaacccagtc atcaaaaggc ggacgttaca
    33901 ctgtcacgct tcctgacggc actaaagtcg aagaactgaa caaagcgaca gcagcgatga
    33961 tggtcccgtt cgacagcatc aaattctccg gcaactacgg caacatgacc gaagtctcct
    34021 atcaggttgc gaaacgtgcc gcgaagaaag gtgctaagta ttaccacatc acccgccagt
    34081 ggcaggaacg tggtaataac ctgaccgtca gcgcagatct gtataaataa ctattcagtt
    34141 actcctataa ggcggcttga tgccgccttt cgcattttta gtcacaaatt tccccgcgac
    34201 cattgcatgc agacacatct cttcgtaaaa tcccgcgctt cagtggcaaa ctccattttt
    34261 atgcgttttg ccctaatatt tattcttctt atcacgtttt aatcactgga tatcgatgga
    34321 aaagaaactg ggactgagcg cactcaccgc gctggtatta agctcaatgc tgggcgcggg
    34381 tgttttcagt ctgccgcaaa atatggcggc agttgccagc ccggcagcac tgctcatcgg
    34441 ctggggtatt actggcgctg gcattttatt gctggccttt gccatgctga tcctcacgcg
    34501 cattcgtccc gaacttgacg gcggtatctt tacctatgcc cgcgaagggt ttggcgagct
    34561 aatcggcttt tgttccgcat ggggatactg gctgtgcgca gtcatcgcca acgtctccta
    34621 tctggtgatc gttttttccg cgttaagctt ttttacggac acgccggaat tgcgcctgtt
    34681 cggcgatggt aatacctggc aatcgatagt cggtgcatcg gctttattgt ggattgttca
    34741 ctttttgatc ctgcgcgggg tgcaaaccgc tgccagcatt aacctggtgg cgacactggc
    34801 aaaattgttg ccgctgggtc tgtttgttgt gctggcgatg atgatgttca aactggatac
    34861 cttcaagctc gacttcaccg gacttgcact tggcgtaccc gtgtgggaac aggtgaaaaa
    34921 caccatgctg atcaccctgt gggttttcat tggtgtggaa ggtgccgtgg ttgtttctgc
    34981 gcgtgcgcgt aataaacgtg atgttggcaa agcgacactg ctggcggttc tctccgctct
    35041 gggcgtttac ttgctggtaa cgctgctttc gctgggcgtg gtggcacgtc ctgaactggc
    35101 tgaaattcgt aacccgtcaa tggccggtct gatggtggaa atgatgggac catggggcga
    35161 aatcatcatc gctgccggtt tgatcgtttc cgtttgcggc gcgtacttaa gctggaccat
    35221 catggcggca gaagttccgt tcctggctgc cactcataaa gcattcccac gcatttttgc
    35281 gcgccagaac gcacaagctg cgccatccgc ctcgctgtgg ttgaccaaca tctgcgtgca
    35341 aatttgtctg gtactcatct ggctgaccgg ttccgattac aacacgttgc tgaccatcgc
    35401 ctcagaaatg attctggtgc cctatttcct ggtcggcgcg ttcttgctga aaatcgcaac
    35461 ccgtccactg cataaagcgg taggtgtcgg tgcctgcatt tatggcttat ggttattgta
    35521 tgcctctggt ccaatgcact tgctgttatc cgttgttctg tatgagccgg ggctgctggt
    35581 tttcctgtat gcgcgcaaaa cgcatacaca tgataatgta ctgaaccgtc aggaaatggt
    35641 gctgattggc atgctgttaa ttgcctcagt tccggcgacc tggatgctgg tgggataacg
    35701 ttcatcccat cgttggatag caaaggagat aacgatgggt aaagcccagc ccttgccaat
    35761 attaattact ggcggaggtc gtcgcatcgg cctcgccctc gcctggcatt tcattaatca
    35821 aaagcaaccg gtgattgtca gctatcggac gcactatcca gccattgatg gactgattaa
    35881 ggcaggtgcg cagtgtattc aggctgattt ttcgaccaac gacggtgtga tggcgtttgc
    35941 cgatgaagta ctaaaaagca cccatggtct gcgtgctatt ttgcataacg ccagtgcgtg
    36001 gatggcggaa aaaccgggtg cgccactggc cgacgtactg gcttgcatga tgcagatcca
    36061 cgttaatacc ccatacctgc tcaaccatgc gctggaaaga ttactgcgtg ggcacggaca
    36121 cgccgccagc gatatcattc actttaccga ttatgtggtg gagcgcggta gcgacaaaca
    36181 tgttgcgtat gctgcaagca aagcggcact ggataatatg acccgctcgt ttgcccgcaa
    36241 gctggcaccg gaagtgaaag tgaattctat tgcgccatcg ctgatcctgt ttaatgaaca
    36301 tgatgatgcc gaatatcgac aacaggcgct gaataaatca ctgatgaaaa ccgcgcctgg
    36361 cgagaaagaa gtgatcgacc tggtcgatta cttacttacc agctgctttg tcaccggacg
    36421 cagtttccca cttgatggcg gtcgtcatct gcgttaatgc agctttatcc agtatataat
    36481 caacacccat gcgcttatcc cccaacacgc aacagaacca acaaaagcgg cgggcaagtg
    36541 catcattccg gtgaaatacc acagcgacac caggtaaaca aaatagggaa taatcgacca
    36601 catactaaaa atgatggttg cgcgtaaggc ttcaatgccg cgttcgctgg caacaatata
    36661 atgcgcgata agcgcaaagg tcgggaaaag tggaatcagc ccggcgatat aataattttt
    36721 cgtttttgct aaaacaccaa tcaacagcac taccagcgca ccgagcgcgg ctttgattac
    36781 cagccccatc tttttacctt aacacttcca taacaagtca tcagtagaat acctgatgaa
    36841 aacttgttta gaaacgattg atagtaagta aaaacagcgc ggtgtattgt gacgttttta
    36901 tatctaccgt gaatgttatg aacactatcg tatttgtgga agatgatgcg gaagtcggtt
    36961 cactgattgc cgcgtacctg gcaaaacatg atatgcaggt taccgtagaa ccgcgcggcg
    37021 accaggccga agaaaccatt ttgcgagaaa atccggattt ggtgttactc gacatcatgc
    37081 taccaggcaa ggacggcatg accatttgtc gtgatttacg cgcaaagtgg tctggaccga
    37141 ttgttcttct aacctctctc gatagcgata tgaaccacat cctggcactg gaaatgggtg
    37201 cctgcgacta tattctcaaa acgacgcccc ctgctgtttt gctagcgcgt ttacgtttgc
    37261 atttgcgtca gaatgagcaa gccacactga ccaaaggtct tcaggaaacg tctctgactc
    37321 cctacaaagc cctgcatttc ggcacgttga ccatcgatcc catcaaccgc gtagtcaccc
    37381 tggttaacac tgaaatctcg ctctcgacag ctgatttcga attattgtgg gaattagcta
    37441 cccatgccgg gcaaatcatg gaccgcgatg cattgctgaa aaatttacgc ggcgtcagtt
    37501 atgacggact ggatcgtagc gtggacgtgg ctatttcgcg gttaagaaaa aaactgctcg
    37561 ataacgccgc agaaccttat cgcattaaaa ctgtgcgtaa caaaggctat ctttttgcgc
    37621 ctcatgcatg ggaataagcg atgaaaaaac tgtttatcca gttttacctg ttattgtttg
    37681 tctgcttcct tgtgatgtct ctgctggttg ggttggtgta caaatttacc gccgaacgcg
    37741 cgggcaaaca gtcgctggat gatttgatga acagttcgct gtatctgatg cgcagcgaat
    37801 tgcgtgagat ccccccacac gactggggta agacgctgaa agagatggat ttaaatctct
    37861 ctttcgatct gcgtgtcgag ccattgagta aataccatct tgatgatatt tccatgcacc
    37921 gattgcgtgg cggcgaaatt gtcgccctgg acgatcagta cacgtttttg cagcgtatcc
    37981 cgcgcagcca ctacgtgctg gcagttggtc ctgttcctta tctttattat ctccatcaga
    38041 tgcgattgtt ggatatcgcc ctgatcgctt ttattgctat ttccctcgcc tttccggtgt
    38101 ttatctggat gcgtccgcac tggcaggata tgttaaaact ggaagcagcg gcgcaacgat
    38161 ttggcgatgg gcatctcaat gagcgtatcc actttgatga gggttcgagc tttgaacgac
    38221 ttggtatcgc atttaaccag atggcggaca atatcaacgc cttgattgcc agcaaaaaac
    38281 agcttattga cggtatcgct cacgaactgc gaacaccgtt agtgcgcctg cgttatcgac
    38341 tggagatgag cgataacctg agcgccgccg aatcccaggc gttgaatcgt gatatcagtc
    38401 aacttgaagc tttaattgaa gagctgctga cttatgcccg actcgatcgc ccacaaaacg
    38461 agcttcatct tagcgaacca gacctgccgt tgtggctgtc aacgcatctg gcagatattc
    38521 aggcagtaac gcccgataaa acggtacgga taaaaacgct cgtgcaaggc cattatgcgg
    38581 cgttggatat gcgcttaatg gagcgcgtgc tggataattt gctcaataac gccctgcggt
    38641 actgccattc aacggttgaa accagcctgc tactgtcggg gaatagagcg acattaattg
    38701 ttgaggatga tggcccaggg attgccccag aaaaccgcga acatatcttt gaaccttttg
    38761 ttcgcctcga tcccagccgg gatcgctcaa ccggcggctg cgggctgggg ctggcaattg
    38821 tccactctat agcactggca atgggcggta cggttaattg tgacaccagc gaactgggtg
    38881 gtgctcgctt ctcgtttagc tggccgttat ggcataacat cccgcaattt acctctgcct
    38941 gacactacgc gcacgatggt caagtcacca cgactgtgct ataaaataag tatgttgtaa
    39001 ctaaagtggt taatattatg gcgcgttacg atctcgtaga ccgactcaac actacctttc
    39061 gccagatgga acaagagctg gctgcatttg ccgctcatct tgagcaacac aagctattgg
    39121 ttgcccgcgt gttctctttg ccggaggtaa aaaaagagga tgagcataat ccgcttaatc
    39181 gtattgaggt aaaacaacat ctcggcaacg acgcgcagtc gctggcgttg cgtcatttcc
    39241 gccatttatt tattcaacaa cagtccgaaa atcgcagcag caaggccgct gtccgtctgc
    39301 ctggcgtgtt gtgttaccag gtcgataacc tttcgcaagc agcgttggtc agtcatattc
    39361 agcacatcaa taaactcaag accacgttcg agcatatcgt cacggttgaa tcagaactcc
    39421 ccaccgcggc acgttttgaa tgggtgcatc gtcatttgcc ggggctgatc acccttaatg
    39481 cttaccgcac gctcaccgtt ctgcacgacc ccgccacttt acgctttggt tgggctaata
    39541 aacatatcat taagaatttg catcgtgatg aagtcctggc acagctggaa aaaagcctga
    39601 aatcaccacg cagtgtcgca ccgtggacgc gcgaggagtg gcaaagaaaa ctggagcgag
    39661 agtatcagga tatcgctgcc ctgccacaga acgcgaagtt aaaaatcaaa cgtccggtga
    39721 aggtgcagcc gattgcccgc gtctggtaca aaggagatca aaaacaagtc caacacgcct
    39781 gccctacacc gctgattgca ctgattaatc gggataatgg cgcgcgcgtg ccggacgttg
    39841 gtgagttgtt aaattacgat gccgacaatg tgcagcaccg ttataaacca caggcgcagc
    39901 cgcttcgttt gatcattcca cggctgcacc tgtatgttgc ggattaacgc ccggctttca
    39961 tactgccgac catctgttct ggccgtaccc agctgtcaaa ctcggcttcg ctaagatacc
    40021 ccagcgcaag ggccgcagct tttaaggtca gcccttcttt atgcgctttt ttggcgatct
    40081 ccgcggcttt gtcataacca atgtgggtgt taagcgcagt caccagcatc agcgattcat
    40141 tgagtaattg attgattcgc tcacgattcg gttcaatacc cactgcgcag tgtttgttaa
    40201 aactttccat gccatctgcc agcaagcgca ccgattgcag gaaattgtgg atcaccattg
    40261 gacggaagac gttcagttca aagttaccgg aagcgccccc catgttgatc gccacgtcgt
    40321 tccccatcac ctgacagcag agcatggtta atgcctcaca ctgtgttgga ttcactttcc
    40381 ccggcatgat tgagctgccc ggctcatttt ccgggattga gatttcacca attccgcagc
    40441 gcgggccaga ggccagccag cggacatcat tggcgatttt catcagtgac gcagccaacc
    40501 ctttcaacgc gccgtgcgcc tgaaccagag catcacaggt cgccagcgct tcaaatttgt
    40561 tcggcgcggt aacaaacggt gcacaggtaa tgactgccag ttcatctgct acgcgacgcg
    40621 catactccgg atgggtattt agtccagtac ccaccgctgt accgccaaga gccagttccg
    40681 ctacgtgagg caggctgtat tcgatatgtt tgagattata ctcgagcatc gctacccagc
    40741 cggaaatctc ctgccccagt gttagcggcg tggcgtcctg caagtgggtt cgaccgattt
    40801 tgacgatatc ggcaaatgca cgcgattttt cactcagcgt ctgtgtcagg gttttaagct
    40861 gcggaatgag ttgcttgcgc agcgccagca gcgccgcaac gtgcatcgcc gtcggaaaga
    40921 catcgttgga actttggctt ttgttcacgt cgtcgttagg gtgaacttta cgttccatcc
    40981 cgcgcacgcc gccgagtaat tcactggccc ggttagccag cacttcgttc atgttcatgt
    41041 tactttgcgt gccggagccg gtctgccaga tagccagcgg gaattcgtcg tcatgctgtc
    41101 ctgccagtac ttcatccgcc gcctgccgaa tggcgctcgc tttctcttca gacaacaagc
    41161 ctaaatcttc attaactttt gccgctgcgc gcttggttag cgccagcgca tgaatcagtg
    41221 aggtgggcat tttctccgtc gaaatgcgga aatgctccag cgagcgttga gtttgtgcgc
    41281 cccacagctt atctgccggg acatcaatcg cccccatcga atctttttcg ctgcgtactg
    41341 tattcatggc ctgctcctca cctgtttaat tttttctttc tgttttgctt tcgttaagca
    41401 acttttagct cacttattat ttactatttg ataacaaatg tttggtcttt cgtgccatgt
    41461 aaaaaaaccg ccccgaaggg cggctctgtt tatttcacac agcgagtgca ctgtgtgagt
    41521 tgtatctgct ggaagaagtc atttccttta tcatccacaa ggataaacgc cgggaaatct
    41581 tccacttcaa ttttccagat ggcttccatt cccagttccg gatattcaac acattccagg
    41641 ctcttaatac ttccctgcgc caatacagcg gccggaccac cgatactgcc aaggtagaag
    41701 ccgccgtgtt ttttacaggc atccgtcacc tgctggctgc ggttgccttt cgccagcatg
    41761 atcatacttc cgccctgcgc ttgcagttga tcgacataag aatccatccg tccagcggtc
    41821 gttgggccaa gagaaccgga ggcataacct tccggcgttt tggccggacc cgcgtagtaa
    41881 atcggatgat ctttaatgta ctgcggcagc ccttcaccgt tatccatccg ctctttcagt
    41941 ttggcgtgag caatatcacg accgacgata atcgtgccgt taagcgataa gcgtgtagaa
    42001 acgggatact gcgacaactg tgcgaggatc tctttcatcg gacggttaag gtcaacgcgc
    42061 accgcttcgc cttctcccgc tttacgcagc tcttcaggga tatatttacc tggattatgt
    42121 tccagttttt cgatccagat cccctgacgg ttgatcttcg ctttgatatt acggtcagca
    42181 gagcaggaaa cacccatacc aaccggacag gatgcgccgt gacgtggcag gcgaatcacg
    42241 cgtatgtcgt gagcgaagta tttaccacca aactgcgcac ccagaccgag attttgcgct
    42301 tcgatcagca attctttttc cagttccaca tcgcggaatg cctgaccgtg ctcattccct
    42361 tccgttggca gttcgtcata gtatttagcg gaagccagtt tcactgtttt aaggttagtt
    42421 tctgcagaag ttccaccaat tacgaacgca atatgatacg gaggacaggc cgccgtaccc
    42481 agcgtgcgca tcttctcaac caggtaattt ttcagtttcc ccggcgttag taacgctttg
    42541 gtttcctgat agagatacgt cttgtttgcc gaaccaccac ctttggcgat acagaggaat
    42601 ttgtactcgt cgccatcaac ggcataaaga tcgatctgcg ctggcagatt ggtgccggta
    42661 ttcacctctt tatacatatc cagcggcgcg ttttgcgagt agcgcaaatt atcttcgata
    42721 taagtgttat agacaccgcg cgccagcgcc gcttcatcac agccaccggt ccatacacgc
    42781 tgcccttttt taccaacaat aatcgcagtg ccggtatcct gacaggttgg cagaacgcct
    42841 ttcgccgcga tgtcggagtt acgcaggaat tgcaacgcca catatttatc attttcgctg
    42901 gcctccgggt cacgcagaat gtcggccacc tgttgttggt gagccggacg cagcataaac
    42961 gacgcatcat gaaacgcctg acgcgccaac agagttaacg cttcgggtgc gactttcaaa
    43021 atctcctgcc cttcaaattc agatacgcta acgtgttcgc tggttagcag gtaatactca
    43081 gtatcatctt ttttgagtgg aaaaggagcc tgataatgaa agggtttgtt tgacattgtt
    43141 ctctcactta ctgcctggtt tggttatgct ctgggcgggt gttccgttgc cctgttaaaa
    43201 gcgagtaaca atatcctaca cactttttta acaaaaactg agactagtac gactttttgc
    43261 ggctccaggt tacttcccgt aggattcttg ctttaatagt gggattaatt tccacattaa
    43321 aacagggatt gatcatgcaa aaactcatta actcagtgca aaactatgcc tggggcagca
    43381 aaacggcgtt gactgaactt tatggtatgg aaaatccgtc cagccagccg atggccgagc
    43441 tgtggatggg cgcacatccg aaaagcagtt cacgagtgca gaatgccgcc ggagatatcg
    43501 tttcactgcg tgatgtgatt gagagtgata aatcgactct gctcggagag gccgttgcca
    43561 aacgctttgg cgaactgcct ttcctgttca aagtattatg cgcagcacag ccgctctcca
    43621 ttcaggttca tccaaacaaa cgcaattctg aaatcggttt tgccaaagaa aatgccgcag
    43681 gtatcccgat ggatgccgcc gagcgtaact ataaagatcc taaccacaag ccggagctgg
    43741 tttttgcgct gacgcctttc cttgcgatga acgcgtttcg tgagttttcc gagattgtct
    43801 ccctactcca gccagtcgca ggtgcacatc cggcaattgc tcacttttta caacagccta
    43861 atgccgaacg tttaagcgaa ctgttcgcca gcctgttgaa tatgcagggt gaagaaaaat
    43921 cccgcgcgct ggcgatttta aaatcggcgc tcgacagtca gcagggtgaa ccgtggcaaa
    43981 cgattcgttt aatttctgaa ttttacccgg aagacagcgg cctgttctcc ccgctgttac
    44041 tgaatgtggt gaaattgaat cctggcgaag cgatgttcct gttcgctgaa acaccgcacg
    44101 cttacctgca aggcgtggcg ctggaagtga tggcgaactc cgataacgtg ctgcgtgcgg
    44161 gtctgacgcc taagtacatt gatattccgg aactggttgc caatgtgaaa ttcgaagcca
    44221 aaccggctaa ccagttgttg acccagccgg tgaaacaagg tgcagaactg gacttcccga
    44281 ttccagtgga tgattttgcc ttctcgctgc atgaccttag tgataaagaa accaccatta
    44341 gccagcagag tgccgccatt ttgttctgcg tcgaaggcga tgcaacgttg tggaaaggtt
    44401 ctcagcagtt acagcttaaa ccgggtgaat cagcgtttat tgccgccaac gaatcaccgg
    44461 tgactgtcaa aggccacggc cgtttagcgc gtgtttacaa caagctgtaa gagcttactg
    44521 aaaaaattaa catctcttgc taagcttatt aaaggcttat aacaccttca ggcggccagt
    44581 ccgcctgatt tcattttatg gataatcatt atgaataaat cgctggtagc ggtaggcgtc
    44641 attgttgcgc taggcgtagt ctggacaggc ggcgcatggt atacaggcaa gaagattgaa
    44701 acccatctcg aagacatggt cgcgcaggcg aacgcgcaac tcaaactgac agctcctgaa
    44761 tccaacctgg aagtgagtta tcaaaactat catcgcggcg tattcagcag ccagttgcaa
    44821 ctgttggtga aacccattgc cgggaaagaa aatccgtgga ttaaaagcgg tcagagcgtc
    44881 atcttcaacg aatcggttga tcatggtccc ttcccgcttg cccagcttaa aaaactgaac
    44941 ctgatcccgt cgatggcatc aattcaaacc acgctggtta ataacgaagt aagcaaacca
    45001 ctgtttgata tggcaaaagg tgaaacgcct tttgagatta actcgcgcat tggttacagc
    45061 ggtgattcca gttccgatat ttcgctcaag ccactgaatt acgagcaaaa ggatgaaaaa
    45121 gtcgccttta gcggcggcga gttccagtta aatgctgaca gagacggcaa agccatctcc
    45181 ctttccgggg aggcgcaaag tggtcggata gacgcagtta acgaatacaa ccagaaagtg
    45241 cagttgacct ttaataatct gaaaaccgac ggttccagca cgctggcaag ttttggtgag
    45301 cgcgtaggaa accaaaaact gtcactggaa aaaatgacca tttcagtgga aggcaaagaa
    45361 ctggcactgc tggaaggcat ggagatcagc ggtaaatcgg atctggtcaa tgacggtaaa
    45421 acgatcaata gccaactgga ttactcgcta aacagcctga aggtacagaa tcaggatctg
    45481 ggcagcggca agctgacttt aaaagtcggc cagattgatg gtgaagcctg gcatcagttt
    45541 agccagcaat ataacgcgca aactcaggcg ctgctggcac agccagaaat tgccaacaat
    45601 cccgaacttt atcaggagaa agtgacggaa gccttcttta gcgccctgcc gctgatgttg
    45661 aaaggcgatc cggtgattac tatcgcgccg ctaagctgga aaaacagtca gggtgaaagt
    45721 gcgctgaatc tgtcgctgtt cctgaaagat ccggcaacga ctaaagaagc gccgcaaacg
    45781 ctggcgcagg aagtagatcg ttcggttaaa tctctggatg cgaaactgac cattccggtg
    45841 gatatggcaa ctgagtttat gactcaggta gcgaagctgg aaggttatca ggaagatcaa
    45901 gcgaaaaaac tggcgaaaca gcaagttgaa ggtgcatcag caatggggca gatgttccgt
    45961 ctgaccacct tgcaggacaa taccatcacc accagcctgc aatatactaa cggtcagata
    46021 acgttaaacg ggcagaaaat gccactggaa gatttcgttg gtatgtttgc aatgccggca
    46081 ttaaatgttc cggtcgtacc cgctattccg cagcagtaat tcacagacaa aacgccggag
    46141 atttttctct ccggcgttat tttttacttc agcataaagt catactgaat actcattttc
    46201 acatcccagt aattcaggcg atcgcccagc gtactgccat cgcgcttttc ccgcccttta
    46261 tcggcagcaa gaaagaaatt catcccttgt aacggccctt cgctgacccg atagttcata
    46321 ccacctaaga tacccgtgcc ttcaatccgc acatctcgtt tactggattc atagccgcga
    46381 gtcacaatcg gtgcaaatgt cagcgtaaat tgcggcgtta aacgatagtt gacgcccaat
    46441 tgccaggact gcattccttc acggttacga tcaagactta atgaaaaggg atagccaatg
    46501 tcggtatcca ccacggcacc attcattgca tggcgcaaat aaccaccact gccaaatacg
    46561 cttccccacg gcgcattcca ggtcaattgt ccactaacca acgccgtttc attgggctgg
    46621 ctggtattac gacttaaacc ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac
    46681 gtataattcg ggtcgagcgg tatgcttttc tcgagaatac tttgtatctg attggaaaca
    46741 tctttctgta ccgatgcccc cagagaatag cgactggtat catcatgcca actggcggct
    46801 aaaaaagaac gtgcgcgatg tttatcgtat tttcccgtct ggctgttata caccccttca
    46861 tcacgaatac cggttgttga atcagtccat aaaaatactc gtgaacgcca cattccccac
    46921 agatcaatat ttggtgttaa agcccaataa cctaatgctc cttcagaaac tgtcggtaat
    46981 gtacgttgcg aactgccagc cattaacgga atacgataag gtaagctacc ataactataa
    47041 tccgtcccga agcgacctaa tttaagcagg ctattttcac tgggttttat ttttactgcc
    47101 ccagggcaaa gccaaaaatt ttatcaccat caagatacca ccgggtactc atatcagcac
    47161 gagcacctaa tttataaaca taatatgccc cgccttcaac gccaataata ttggaataat
    47221 aaccactatt gaaatcgagc aatcctccct gtacccatgc gtaaatatct cttcccgcgc
    47281 cagcactggg cttatccgcc ctgcgcaatt cgtttttcag tctgatgcgt agtcccgctt
    47341 catcgtcagc cagttgcgcc gcataagcag aggtaaggcc agaggcagcc gtcaggcaaa
    47401 taacggccat ggccactatt tttctcatag ttgcaccttt gatgttctga ttatttttat
    47461 tgaatattaa ttagtgatat cgctgattaa ttgctgctgc acttttttac gattatcaat
    47521 ttcaaccacg atttctttga attttttatc cgtgagcgga taaaaccaga taataacgaa
    47581 tgccagtagc ataaatccgc aaggtactaa ggcaattgat gtgcggatgc ccataataac
    47641 ttccggcgtt tgcacctgat tggcgatata tccgcttaat cccaaaataa aggcaggaat
    47701 tgaacctccg attgcctgac cgcatttacg ggtaaatgag aatagtgaat aggtgagccc
    47761 ttcaattcgc acgccggtca ggtattcacc gtattctacg gtatcagctt ccagtgccca
    47821 catcacagtc atagcaacgc cctgaccaat tgaagcgatg gccaacgcaa ccaacgccac
    47881 cggcagtgac cagacggaaa cccagaagaa tagcagataa ccgcaggttc ccagcaaagc
    47941 gccaatcagg aaggtattct ttttaccgat cctcgcgacc atccccggca ccagcggtgc
    48001 cgatgccaca gtaccaacca ggttttgcac cagtaccagc acagtgaaca gcccggtatc
    48061 atttaacaca tagcgcacgt agaacaacga cgaggcgctg accgcgaagg tcgaaatcag
    48121 cacacacagc gcaccgatgc acaacataaa cagcgggcga ttccgtttca gggtttgcag
    48181 actgatcttc aatgacggct gcgccacgat acgtaccaca ttctcacgcg tcgatttgaa
    48241 gcagatgaag taaagcacca ttccggcaat cgccagcaca atcgtccaga aatggtatac
    48301 cgacaccatc tcttccgggc tggagttctt aatgctcggt cctatcagaa atgccaggca
    48361 gacaaaggtt aatgaagcgg caatcccgcg agccgcgccc agacgggcgc gggattgtgg
    48421 ttgttgggtc atcgcggtag caagtgaacc ataaggaata ttcaccaggc tgtagcaaag
    48481 cccgaggccc atgtaggtca aatatgcata caccactttg ctaccatggc tccagtcggt
    48541 cggcacccag aataccagca cgctgaagat cattaacggc gcagtaccga agagtaaaaa
    48601 cgggcggaat tttccccagc gggtattcac actgtccacc actcgtccgg caaagacgtc
    48661 ggcgaaggca tcgaataccc gcaccagtaa cagcatggtg cccgccgcag cggcaccgac
    48721 gccagcgacg tcggtgtagt aactcaatag gaagagcgcc cccattgcga aggcgaagtt
    48781 attggcgacg tcaccgaggc tgtagccgac gatggtgcgc caggagagtt gttgattcat
    48841 tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc
    48901 agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca
    48961 acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg
    49021 acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct
    49081 tcactccaca tgtcggtgta cattgagtgc agcccggcta atgtatccac gccgtattcg
    49141 gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc
    49201 ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac
    49261 agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg
    49321 caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat
    49381 tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt
    49441 gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt
    49501 tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca
    49561 atgcctaaag agaggttaaa gccgacagca gcagtttcat caatcaccac gatgccatgt
    49621 tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg
    49681 gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg
    49741 ccacgcaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta
    49801 atcaggaact gttcgccctt cactgccact gaccggatgc cgacgcgaag cgggtagata
    49861 tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt
    49921 tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc
    49981 acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag
    50041 tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc
    50101 caggtgttcg gcgtggtgta gagcattacg ctgcgatgga tcccggcata gttaaagaaa
    50161 tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg
    50221 atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca
    50281 ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact
    50341 tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata
    50401 cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca
    50461 taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc
    50521 cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc
    50581 agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga
    50641 cgtaacataa gggactcctc attaagataa aaatactggt caacctttaa tctgattagt
    50701 taaccaattc tgtgttgcac acagttatct gtgagaaacc catggaagag aaatggaaat
    50761 aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca atacccacag cgtgatattg
    50821 atcgcattaa tctttaaaac ggcgttgaca gaagccataa aacaagcaac cgaacgttcg
    50881 ttcgagtata ttgggaacga taaactctca tgacatatgg ctacggtgaa tatttttggg
    50941 ggggaattct ccgatcccgg ctagattgta ttttgtacag ggaaattaaa gtatgaaacg
    51001 cgggcacagt tatcaccagc ccgcgaaatg agaggatcag gatgcggtta agataccgcc
    51061 aatcatcgcg cgtaatccct gagcaaacga cgcttcacgc gccagatctt ccgcttcgat
    51121 atcggccagt acaccgtagg tcagatccag cagtaaacgt gaagtcattg acgtgttgat
    51181 gtctgggctt atctcgcctt tttgctgggc atcacgcatc cgctgggcaa caaattcagt
    51241 gagcgtcata tgcttatttt taagcatggc ggcgacctgt gggttacgca tcccttccgc
    51301 cataatttcg accaccagcg cccgttgccc aaaggcttca tgggtgagag agacaataga
    51361 ctcgaccata tagtcaacaa aatgaatccc ttcaatcggt tcccggaaac gggccagcgc
    51421 cctctcctgg tcctgtaaga taatcgcctg aatcaaggct tctttggaga tgaaatggtg
    51481 atagagcgtc ccgggactaa tggcgcagga tttacagatg gctttcatcg aggcactgtg
    51541 aaatccattt tctgaaaaaa tctctctggc agcattgagg atccgggtcc gtgtcggttg
    51601 tgcttcagtc tgcatgttat ccatcattaa accattatag ttagcgtgaa taactagttt
    51661 accgcgttgt caggtgagcg cctatcattg ctgttgaata gtgcgaaggc acactctatt
    51721 cgtatgcttt tcatccagct attggttaac caatagccgt gatttggctg ccagttgttt
    51781 agcagagacg ttgatgaatt gcacacgcta tcccggtaaa caacacatac ggctgtttac
    51841 cggttcgtta gtgtattaat tgagctcctg taccccacca ccggagacgg tgagaatttg
    51901 tccgcttacc cagctggctg caggcgagca aaggaacagc gccgcgttag caatatcttg
    51961 cggttggccc agacgtctga tcggcgtgtg ctgtaacatt ttttgttcaa tttctggtgt
    52021 aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgccat tcacccgaat
    52081 atttttttca cccaggtcaa atgccatatt tctgaccaga tgactggccg cagctttaga
    52141 tgatgcatag gaagtcatgt ttatattttt attttctgcc gccatagaag tgatggtcag
    52201 aataacgcca ccgccatttt tttccatttc tggcgcaaca agttgtgaca gatggaaaaa
    52261 agaaaacaca ttcagttcat aagcacggcg aaaatctgcc attggcatat caaacggttt
    52321 aggaccaccg ccaccggcgt tgttaaccag gatatcaact ttacccagct tactgacggc
    52381 aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcacaacggc aggcaaatgc
    52441 ctgaccaccc agttgttgaa tttcgtctac aacatggtta gctgcgtcgg cgttaatatc
    52501 actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt taccaatacc
    52561 tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt cagaattaaa
    52621 cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctgtgt gccacatttt
    52681 ttatcattaa ccttgaggaa aggacactac caggtatagc agataaagag aggattattt
    52741 cgccgcaatg agccgggcgg gaataataag attgcgtgaa tgcgtctctt catgggtgat
    52801 tttttgcatc atgcgatccg caagtgtgat accaagttcc cgcgctggcg tgctggccca
    52861 ggtaacaggt atatcatcaa gtgtggttgg tgtcgcatcg gtaaatgccg ccagcgaaac
    52921 ctgttgctca aagtaacgat cgactccgct ttccccgctt tgccgccctg ctttgagcaa
    52981 accaaaccat gcccccatcg caatagtttc gttatagcaa accacggcac tgatggtcgg
    53041 gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc ttctggctgg aagtgcactc
    53101 caacacccaa tcgctatgaa acggcaggcc aaattttagt agagttgcac aatagccccc
    53161 cacccgttct gcacgggtta atgaggaact ttgccctccc agccaggcga tccgctgatg
    53221 cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc tgcatgttgt ccgggcgaac
    53281 cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa atcaccggga tagctttttc
    53341 ttctgccatc cgtcgcaggt catcgctact tcctgcagct ccggcaatta ccacaccatc
    53401 gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc tgctcaccgt ctttaccgcc
    53461 gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct tccgtcaatc cggccgtcaa
    53521 ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa ccaatgacgc cgctttgccc
    53581 gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc agctcttcaa tggcggcgtt
    53641 aacgcgttct cctgtggcgg tagagattcg ccctttgcca ctaagcacca gcgaaacggt
    53701 acttaccgac acgcccgcag ccagcgcaac atcatgaatg gttatttttt tggcggtagc
    53761 catgcagatg acctactccc tgattatgtg acagataaaa cgttttacct tttatttatc
    53821 ttatacccgc tattatcgtt gcgtaatgtg atttatgcct cactaaaatt tgataaaacg
    53881 ttttatcttc tcgcgcaatt tactgaatcc agattgttct ctacgaggag tcgttttatg
    53941 acggcgaaaa cagcaccgaa agtcacgctg tgggagttct tccagcagtt aggcaaaacc
    54001 ttcatgttac ccgtggcatt attgtcgttc tgcggcatta tgctcggcat tggtagttct
    54061 cttagcagcc atgatgtcat aaccctgatc ccggtcctgg gcaaccccgt gttgcaggct
    54121 atctttacct ggatgagtaa gattggctcg tttgctttta gtttcctgcc tgtcatgttc
    54181 tgtatcgcca tcccgctggg cctggcacgc gaaaataaag gcgtagcggc attcgctggc
    54241 ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct ggttgaccaa taaaggcatt
    54301 ctgccaacca cggatgccgc ggttctgaaa gccaataaca tccagagcat tcttgggatc
    54361 cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg gtattatcgt ctggatgctg
    54421 catgagcgtt tccataatat ccgcctgccg gatgcgctgg cattcttcgg cggtacgcgc
    54481 ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg gcctggtgat tccattagtc
    54541 tggccgattt tcgccatggg tattagcggc ttaggccata tgatcaacag cgcgggtgat
    54601 ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt tgccgtttgg tctgcatcac
    54661 attctggtgg cattaattcg ctttaccgac gcaggcggca cgcaggaagt ctgcggtcaa
    54721 accgtcagcg gcgcactgac catcttccag gcgcaattga gttgcccgac cactcacggt
    54781 ttttctgaaa gcgccacgcg tttcctttcg caaggcaaaa tgcctgcgtt tctcggcggt
    54841 ctgccaggtg cagcgttagc tatgtatcac tgcgcgcgcc cggaaaatcg ccataaaatt
    54901 aaagggctgc tgatttctgg cctgatcgcc tgtgtcgttg gcggcacgac cgaaccgctg
    54961 gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca tccacgcgct gttaaccggc
    55021 ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg gtaataccga cggcaatatc
    55081 atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa ccaagtggta catggtgcca
    55141 gtggtggcgg caatctggtt cgtcgtttac tacgtcatct tccgtttcgc tatcacccgc
    55201 ttcaatctga aaaccccggg gcgcgatagc gaagtggcca gttcaatcga aaaagccgtt
    55261 gccggtgcgc cgggtaaatc aggttacaac gttccggcaa tcctcgaagc cttaggcggt
    55321 gccgacaata ttgtcagcct cgataactgc attacccgtc tgcgtttgtc tgtgaaagat
    55381 atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg caattggcgt agtacaactt
    55441 aatcaacata acctgcaggt tgttatcggg ccacaagttc agtcagtaaa agatgaaatg
    55501 gccggtctga tgcatactgt ccaggcataa ggataagata tgttcgattt ttcaaaggtc
    55561 gtggatcgtc atggcacatg gtgtacacag tgggattatg tcgctgaccg tttcggcact
    55621 gctgacctgt taccgttcac gatttcagac atggattttg ccactgcccc ctgcattatc
    55681 gaggcgctga atcagcgcct gatgcacggc gtatttggct acagccgctg gaaaaacgat
    55741 gagtttctcg cggctattgc ccactggttt tccacccagc attacaccgc aattgatacg
    55801 cagacggtgg tgtatggccc ttctgtcatc tatatggttt cagaactgat tcgtcagtgg
    55861 tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct atgacgcatt ttacaaggcc
    55921 attgaaggta accagcgcac agtaatgccc gttgctttag agaagcaggc tgacgactgg
    55981 ttttgcgata tgggcaagct ggaagccgtg ttggcgaaac cagaatgtaa aattatgctc
    56041 ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt gcgatgagct ggagatcatg
    56101 gctgacctat gcgagcgtca tggtgtgcgg gttatttccg atgaaatcca tatggatatg
    56161 gtctggggcg agcagccgca tattccctgg agtaatgtgg cgcggggaga ctgggcgttg
    56221 ctgacgtcgg gctcgaaaag tttcaatatt cccgcgctga ccggtgctta cgggattata
    56281 gaaaatagca gcagccgtga tgcctattta tcggcactga aaggacgtga tgggctttct
    56341 tccccttcgg tactggcgtt aactgcccat atcgccgcct atcagcaagg tgcaccgtgg
    56401 ctggatgcct tacgcgtcta tctgaaagat aacctgacgt atatctcaga taaaatgaac
    56461 gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca cttatctggc ctggcttgat
    56521 ctacgtctgt tgaatattga cgacaacgcg ctgcaaaaag cgcttatcga gcaagaaaaa
    56581 gtcgcgatca tgccggggta tacctatggt gaagaaggtc gtggttttgt ccgtctcaat
    56641 gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg gattaataaa cgccatccgc
    56701 gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt gcgcaatcgt ggatttttac
    56761 cctgctttgt ttttataatg gtgcgcactt ttatattcag aaaaagagtg cgaccatgat
    56821 tgataccacc ctgccattaa ctgatatcca tcgccacctt gatggcaaca ttcgtcccca
    56881 gaccattctt gaacttggcc gccagtataa tatctcgctt cctgcacaat ccctggaaac
    56941 actgattccc cacgttcagg tcattgccaa cgaacccgat ctggtgagct ttctgaccaa
    57001 acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc tgtcgccgcg tggcatttga
    57061 aaacattgaa gatgcagccc gtcacggcct gcactatgtc gagctgcgtt tttcaccagg
    57121 ctacatggca atggcacatc agctgcctgt agcgggtgtt gtcgaagcgg tgatcgatgg
    57181 cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag cttatcggca ttatgagccg
    57241 gaccttcggc gaagccgcct gtcagcaaga gctggaggcc tttttagccc accgtgacca
    57301 gattaccgca cttgatttag ccggtgatga acttggtttc ccgggaagtc tgttcctttc
    57361 tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag gcgaagctgc
    57421 cgggccggaa agcatctggc aggcgattcg tgaactgggt gcggagcgta ttggacatgg
    57481 cgtaaaagcc attgaagatc gggcgctgat ggattttctc gccgagcaac aaattggtat
    57541 tgaatcctgt ctgacctcca atattcagac cagcaccgta gcagagctgg ctgcacatcc
    57601 gctgaaaacg ttccttgagc atggcattcg tgccagcatt aacactgacg atcccggcgt
    57661 acagggagtg gatatcattc acgaatatac cgttgccgcg ccagctgctg ggttatcccg
    57721 cgagcaaatc cgccaggcac agattaatgg tctggaaatg gctttcctca gcgctgagga
    57781 aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa aatggatggt gcaaattgca
    57841 ccatccattt ttcatgcaag gcacaaagtc gcgcgatgtt tggcggattc gatgcccagc
    57901 tcaatcaact ccattgcctg gattgcctgg cttgccggaa ccggattttc accatcgcca
    57961 tttaacgcat cacgaatagc cgcatagtaa gccggataat tcccaggcac cgtcaacagc
    58021 gtttcttcga cacgttcctc accttccacg cgggtaagta cgccatcacg catatcgtag
    58081 ccccagtctt cctgcggtag acgctcgcca tttttcagac gttcttcctg tggatcgagg
    58141 ccatatttca cataactgcc tcgggatcca tgcacgatat accgtgctga ctcagcggct
    58201 gccagcatgg taccgtgtaa aatgactcgc cgctgtggat aggacaagat ggcgtggaaa
    58261 taatcggtcg actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat gctgaccggt
    58321 aaaccaaata gcgtaatggc ctgatcaaga agatgcggtg ctaaatcgta ccagataccg
    58381 ctgcctgggc cgccctgttc acgccaacga tcgcgcacct gcggacggaa gcggtcaaaa
    58441 tgagactcaa agtaagcaac ttcacccagc acgccttccg cgagcaaacc ttttagcgtc
    58501 aagaaatcgc tatcccaacg acggttatgg aatacagaca gcacacgccc caggcttttt
    58561 gccagcgcat ccagctctcg cgcttgtgac agtgtcacgg taaagggttt atcaacgacc
    58621 acatgtttac ccgcctcaag cgccgctttg gctaacggga aatgggtatc gttgggtgta
    58681 ggaatgacaa tcaggtctat gttgggatcg ttaaacagat gcttcggctc agagacaacc
    58741 gtaaccgttg gccagtcggc ttttactttt gtttcatcac tgctggagat taccgccagt
    58801 tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg ttttgctcgc ataaccatac
    58861 ccaatcaacc caacacggat gttgtcgctc atgtcattgc ctctcattga agtatgatgg
    58921 ctatttgaca ctatccttta cccacgctca acagtttaat aacctgccag caataaggga
    58981 tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa cgacaaaagc taaatatcgc
    59041 aaaaacctca gtaaaaatct tgctggagct attattgcta agtaacattt acaccctgaa
    59101 gttaatggat caatcaagag agatgtgggc tgtaatgaat cgtcttattg aattaacagg
    59161 ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca ttgacaacta
    59221 tcagccacct gaacagagtg cttcggtaca acacaagtaa gctctgcgct tgtagagcgc
    59281 catgctgccc gtccgggtgc atgttttcac ttgtcggata ttaaaccagg aatttattat
    59341 cttgttcgat gttgttggtg attgtcaggg atagtaaagc gcgttactct tttcagagca
    59401 gtttttcatc cctgaatatt tgccatcagc gcattcttat tattgtaata acacaagcgt
    59461 tcacttttgg tgaaatttta tcatcactaa taatacctac ccctttggta ttaattattt
    59521 tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat cagttcactc
    59581 gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga tcaggaactg
    59641 atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg cgggcgtttg
    59701 tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag aaagtagcga
    59761 tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt tgcgtcgcta
    59821 ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt tgcttggccc
    59881 gttggcctgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc tgtataccgc
    59941 tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga cgacacaaat
    60001 cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac tgtggttgac
    60061 catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct ggctgcttat
    60121 ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg gcatagccgt
    60181 tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt tcaaacgctc
    60241 gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac ctgttgtcgc
    60301 ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa atgactgact
    60361 acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc aagtttctcg
    60421 gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc atggggctgg
    60481 caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat acgtggattt
    60541 tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg attgctgtgg
    60601 tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac cgtctgctgg
    60661 ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg ttgctgaata
    60721 tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc gctgtcggtt
    60781 tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg gctgatgtcc
    60841 cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg tctctggcct
    60901 ttatgggctt tagtggtttg gtgaagttgt aatgaatgct atctggattg ccgttgccgc
    60961 cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc gccgttttgc
    61021 ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga gccagtgtgg
    61081 tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta acggtgaaaa
    61141 aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg agttgcttaa
    61201 tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc ggatggtggc
    61261 ggttattgat gaaaataact gtattggctg cactaaatgt attcaggcgt gtccggtaga
    61321 cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct gtacgggctg
    61381 caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg tcgcagaaac
    61441 acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc ccgtggaaca
    61501 ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc aacggcggca
    61561 tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag gtaccactgg
    61621 cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag ttgtgcgtta
    61681 gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc aaaatgctgc
    61741 ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct acggctcatc
    61801 cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac tgctggatcc
    61861 cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc gagcgcatac
    61921 atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt aaattgcagg
    61981 gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag ccgtacatta
    62041 ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt attcgcattc
    62101 ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac aaaccgcagg
    62161 cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg cgggtgattc
    62221 caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc gggaagcagg
    62281 ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc ggcactgctt
    62341 atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt gtaaccctga
    62401 ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg ccagtgcgtc
    62461 atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt atgggtggcc
    62521 cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt accaactgtc
    62581 tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc tgcatccggt
    62641 gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg tactggttca
    62701 gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc attgaatgtg
    62761 gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc cgtcaggaaa
    62821 aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc aaagcgcgtt
    62881 tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa cgacataaga
    62941 gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct ctggcgcggg
    63001 tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc gaacgcccgg
    63061 ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga gcgaaacagg
    63121 cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa actgccgttg
    63181 aagcagctat cgcccgcgcc
//
LOCUS       DAZFAH010000007        58640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFAH010000007 DAZFAH010000000
VERSION     DAZFAH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.007"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..524
                     /gene="cmtB"
                     /locus_tag="ACHJWB_000384"
     CDS             81..524
                     /gene="cmtB"
                     /locus_tag="ACHJWB_000384"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4312075.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            552..1940
                     /gene="cmtA"
                     /locus_tag="ACHJWB_000385"
     CDS             552..1940
                     /gene="cmtA"
                     /locus_tag="ACHJWB_000385"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4312076.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1955..3232
                     /gene="yggP"
                     /locus_tag="ACHJWB_000386"
     CDS             1955..3232
                     /gene="yggP"
                     /locus_tag="ACHJWB_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4312077.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3229..4194
                     /gene="glpX"
                     /locus_tag="ACHJWB_000387"
     CDS             3229..4194
                     /gene="glpX"
                     /locus_tag="ACHJWB_000387"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4312078.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4216..4725
                     /gene="fumE"
                     /locus_tag="ACHJWB_000388"
     CDS             4216..4725
                     /gene="fumE"
                     /locus_tag="ACHJWB_000388"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4312079.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4722..5435
                     /locus_tag="ACHJWB_000389"
     CDS             4722..5435
                     /locus_tag="ACHJWB_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4312080.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5407..6084)
                     /locus_tag="ACHJWB_000390"
     CDS             complement(5407..6084)
                     /locus_tag="ACHJWB_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4312081.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6078..6755)
                     /locus_tag="ACHJWB_000391"
     CDS             complement(6078..6755)
                     /locus_tag="ACHJWB_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4312082.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6743..7450)
                     /locus_tag="ACHJWB_000392"
     CDS             complement(6743..7450)
                     /locus_tag="ACHJWB_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4312083.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7451..8029)
                     /locus_tag="ACHJWB_000393"
     CDS             complement(7451..8029)
                     /locus_tag="ACHJWB_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4312084.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8052..8483)
                     /locus_tag="ACHJWB_000394"
     CDS             complement(8052..8483)
                     /locus_tag="ACHJWB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4312085.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8855..9874
                     /gene="epd"
                     /locus_tag="ACHJWB_000395"
     CDS             8855..9874
                     /gene="epd"
                     /locus_tag="ACHJWB_000395"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4312086.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEGGFANRMLDTTLAMA
                     TVAFR"
     gene            9924..11087
                     /gene="pgk"
                     /locus_tag="ACHJWB_000396"
     CDS             9924..11087
                     /gene="pgk"
                     /locus_tag="ACHJWB_000396"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4312087.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11302..12381
                     /gene="fbaA"
                     /locus_tag="ACHJWB_000397"
     CDS             11302..12381
                     /gene="fbaA"
                     /locus_tag="ACHJWB_000397"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4312088.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12445..12543)
                     /locus_tag="ACHJWB_000398"
                     /pseudo
     CDS             complement(12445..12543)
                     /locus_tag="ACHJWB_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12739..13599
                     /gene="mscS"
                     /locus_tag="ACHJWB_000399"
     CDS             12739..13599
                     /gene="mscS"
                     /locus_tag="ACHJWB_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4312089.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13738..14373
                     /gene="argO"
                     /locus_tag="ACHJWB_000400"
     CDS             13738..14373
                     /gene="argO"
                     /locus_tag="ACHJWB_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4312090.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQCIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14466..15206
                     /gene="yggE"
                     /locus_tag="ACHJWB_000401"
     CDS             14466..15206
                     /gene="yggE"
                     /locus_tag="ACHJWB_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4312091.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15373..16269
                     /gene="ygfI"
                     /locus_tag="ACHJWB_000402"
     CDS             15373..16269
                     /gene="ygfI"
                     /locus_tag="ACHJWB_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4312092.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16266..17744)
                     /gene="scpC"
                     /locus_tag="ACHJWB_000403"
     CDS             complement(16266..17744)
                     /gene="scpC"
                     /locus_tag="ACHJWB_000403"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4312093.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17768..18553)
                     /gene="scpB"
                     /locus_tag="ACHJWB_000404"
     CDS             complement(17768..18553)
                     /gene="scpB"
                     /locus_tag="ACHJWB_000404"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4312094.1"
                     /translation="MSYQYVNVVTINKVAVVEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18564..19559)
                     /gene="meaB"
                     /locus_tag="ACHJWB_000405"
     CDS             complement(18564..19559)
                     /gene="meaB"
                     /locus_tag="ACHJWB_000405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4312095.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     DTEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19552..21696)
                     /gene="scpA"
                     /locus_tag="ACHJWB_000406"
     CDS             complement(19552..21696)
                     /gene="scpA"
                     /locus_tag="ACHJWB_000406"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4312096.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDNAAVTAALNVLTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRSRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21900..22793)
                     /gene="argP"
                     /locus_tag="ACHJWB_000407"
     CDS             complement(21900..22793)
                     /gene="argP"
                     /locus_tag="ACHJWB_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4312097.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23176..23835
                     /gene="rpiA"
                     /locus_tag="ACHJWB_000408"
     CDS             23176..23835
                     /gene="rpiA"
                     /locus_tag="ACHJWB_000408"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4312098.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLSVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24091..25323
                     /gene="serA"
                     /locus_tag="ACHJWB_000409"
     CDS             24091..25323
                     /gene="serA"
                     /locus_tag="ACHJWB_000409"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4312099.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25552..25611
                     /gene="ibsC"
                     /locus_tag="ACHJWB_000410"
     CDS             25552..25611
                     /gene="ibsC"
                     /locus_tag="ACHJWB_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4312100.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25944..26003
                     /locus_tag="ACHJWB_000411"
     CDS             25944..26003
                     /locus_tag="ACHJWB_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4312101.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26104..26706)
                     /gene="fau"
                     /locus_tag="ACHJWB_000412"
     CDS             complement(26104..26706)
                     /gene="fau"
                     /locus_tag="ACHJWB_000412"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4312102.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26727..26910)
                     /gene="ssrS"
                     /locus_tag="ACHJWB_000413"
     ncRNA           complement(26727..26910)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJWB_000413"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26952..27281)
                     /gene="zapA"
                     /locus_tag="ACHJWB_000414"
     CDS             complement(26952..27281)
                     /gene="zapA"
                     /locus_tag="ACHJWB_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4312103.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27449..28027
                     /gene="ygfB"
                     /locus_tag="ACHJWB_000415"
     CDS             27449..28027
                     /gene="ygfB"
                     /locus_tag="ACHJWB_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4312104.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28053..29378
                     /gene="pepP"
                     /locus_tag="ACHJWB_000416"
     CDS             28053..29378
                     /gene="pepP"
                     /locus_tag="ACHJWB_000416"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4312105.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29375..30553
                     /gene="ubiH"
                     /locus_tag="ACHJWB_000417"
                     /gene_synonym="visB"
     CDS             29375..30553
                     /gene="ubiH"
                     /locus_tag="ACHJWB_000417"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4312106.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGTLPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30576..31778
                     /gene="ubiI"
                     /locus_tag="ACHJWB_000418"
                     /gene_synonym="visC"
     CDS             30576..31778
                     /gene="ubiI"
                     /locus_tag="ACHJWB_000418"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4312107.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31852..31935)
                     /locus_tag="ACHJWB_000419"
     CDS             complement(31852..31935)
                     /locus_tag="ACHJWB_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312108.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            32225..33319
                     /gene="gcvT"
                     /locus_tag="ACHJWB_000420"
     CDS             32225..33319
                     /gene="gcvT"
                     /locus_tag="ACHJWB_000420"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4312109.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33343..33732
                     /gene="gcvH"
                     /locus_tag="ACHJWB_000421"
     CDS             33343..33732
                     /gene="gcvH"
                     /locus_tag="ACHJWB_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4312110.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33851..36724
                     /gene="gcvP"
                     /locus_tag="ACHJWB_000422"
     CDS             33851..36724
                     /gene="gcvP"
                     /locus_tag="ACHJWB_000422"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4312111.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILSNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36985..37728
                     /gene="ygfF"
                     /locus_tag="ACHJWB_000423"
     CDS             36985..37728
                     /gene="ygfF"
                     /locus_tag="ACHJWB_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4312112.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGVIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37785..39218)
                     /gene="bglA"
                     /locus_tag="ACHJWB_000424"
     CDS             complement(37785..39218)
                     /gene="bglA"
                     /locus_tag="ACHJWB_000424"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4312113.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            39263..39574
                     /gene="yqfB"
                     /locus_tag="ACHJWB_000425"
     CDS             39263..39574
                     /gene="yqfB"
                     /locus_tag="ACHJWB_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4312114.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39738..40397
                     /gene="yqfA"
                     /locus_tag="ACHJWB_000426"
     CDS             39738..40397
                     /gene="yqfA"
                     /locus_tag="ACHJWB_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4312115.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40593..41573)
                     /gene="ygfZ"
                     /locus_tag="ACHJWB_000427"
     CDS             complement(40593..41573)
                     /gene="ygfZ"
                     /locus_tag="ACHJWB_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4312116.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41816..42082
                     /gene="sdhE"
                     /locus_tag="ACHJWB_000428"
     CDS             41816..42082
                     /gene="sdhE"
                     /locus_tag="ACHJWB_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4312117.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42063..42470
                     /gene="ygfX"
                     /locus_tag="ACHJWB_000429"
     CDS             42063..42470
                     /gene="ygfX"
                     /locus_tag="ACHJWB_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4312118.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42510..43031)
                     /gene="fldB"
                     /locus_tag="ACHJWB_000430"
     CDS             complement(42510..43031)
                     /gene="fldB"
                     /locus_tag="ACHJWB_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4312119.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43143..44039
                     /gene="xerD"
                     /locus_tag="ACHJWB_000431"
     CDS             43143..44039
                     /gene="xerD"
                     /locus_tag="ACHJWB_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4312120.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44064..44774
                     /gene="dsbC"
                     /locus_tag="ACHJWB_000432"
     CDS             44064..44774
                     /gene="dsbC"
                     /locus_tag="ACHJWB_000432"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4312121.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44780..46513
                     /gene="recJ"
                     /locus_tag="ACHJWB_000433"
     CDS             44780..46513
                     /gene="recJ"
                     /locus_tag="ACHJWB_000433"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4312122.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     ILNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLARSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46604..47702
                     /gene="prfB"
                     /locus_tag="ACHJWB_000434"
     CDS             join(46604..46678,46680..47702)
                     /gene="prfB"
                     /locus_tag="ACHJWB_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4312123.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDVLEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47712..49229
                     /gene="lysS"
                     /locus_tag="ACHJWB_000435"
     CDS             47712..49229
                     /gene="lysS"
                     /locus_tag="ACHJWB_000435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4312124.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49272..49820)
                     /gene="idi"
                     /locus_tag="ACHJWB_000436"
     CDS             complement(49272..49820)
                     /gene="idi"
                     /locus_tag="ACHJWB_000436"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4312125.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVSRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            49875..49946
                     /locus_tag="ACHJWB_000437"
     CDS             49875..49946
                     /locus_tag="ACHJWB_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312126.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49943..50068)
                     /gene="yqfG"
                     /locus_tag="ACHJWB_000438"
     CDS             complement(49943..50068)
                     /gene="yqfG"
                     /locus_tag="ACHJWB_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4312127.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50070..51518)
                     /gene="uacT"
                     /locus_tag="ACHJWB_000439"
     CDS             complement(50070..51518)
                     /gene="uacT"
                     /locus_tag="ACHJWB_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4312128.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRRLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51954..53873
                     /gene="ygfT"
                     /locus_tag="ACHJWB_000440"
     CDS             51954..53873
                     /gene="ygfT"
                     /locus_tag="ACHJWB_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4312129.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNDIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQNGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53873..54361
                     /gene="ygfS"
                     /locus_tag="ACHJWB_000441"
     CDS             53873..54361
                     /gene="ygfS"
                     /locus_tag="ACHJWB_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4312130.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRLQRIVASGEN
                     PL"
     gene            complement(54397..55764)
                     /gene="ghxQ"
                     /locus_tag="ACHJWB_000442"
                     /gene_synonym="ygfQ"
     CDS             complement(54397..55764)
                     /gene="ghxQ"
                     /locus_tag="ACHJWB_000442"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4312131.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55800..57116)
                     /gene="guaD"
                     /locus_tag="ACHJWB_000443"
     CDS             complement(55800..57116)
                     /gene="guaD"
                     /locus_tag="ACHJWB_000443"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4312132.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKTWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57134..58533)
                     /gene="xanQ"
                     /locus_tag="ACHJWB_000444"
                     /pseudo
     CDS             complement(57134..58533)
                     /gene="xanQ"
                     /locus_tag="ACHJWB_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
BASE COUNT        14304 a        14611 c        15702 g        14023 t
ORIGIN      
        1 ctcaattgct ttcattaaac cgcacgagta ttttattttt accgccggac ggtaccctaa
       61 aaataaaata aggataattt atgcggctta gtgattattt tccagaatca tcaatctcag
      121 ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta tcattgctgg
      181 ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc attaacaatg
      241 gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg gaatgtgggg
      301 cgcttaaaac cgggatgtct ttgacattac ttgaacaagg tgtttatttt ccggggaatg
      361 acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg cacattggcg
      421 ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa ctcttaacag
      481 catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc tctcctctac
      541 aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt gggggctttt
      601 tgactgcaat ggtcatcccc aatattggtg cttttattgc ctggggtttt attactgcgt
      661 tatttattcc caccggttgg ctgcctaacg aacatttcgc caaaattgtc ggcccgatga
      721 ttacctattt attgcccgtg atgattggtt ctacaggtgg tcatctggtc ggcggtaaac
      781 gcggggcggt catgggcgga ataggtacta ttggtgtgat cgttggcgca gagatcccga
      841 tgttccttgg ctcaatgatc atggggccgc tcggtgggtt ggtcataaaa tatgtcgata
      901 aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac ttctcattag
      961 gtatcgcggg gatgctcctt tgtctgctgg gttttgaagt gatcggcccg gcggtgttaa
     1021 ttgccaatac tttcgtcaaa gagtgtatcg aggcgctggt acatgcgggt tatctgcctc
     1081 tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg atcgatcagg
     1141 gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc atcttcttta
     1201 tggtggcctc taacccaggt ccgggcctgg ggctgctgct ggcgtttacc ttgtttggta
     1261 aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc ggtgggatcc
     1321 acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc atgattgcgg
     1381 gcggtatgtc tggtacctgg atgtttaact tactggacgg tggtctggtg gctggcccaa
     1441 gtccggggtc tatctttgct tacctggcac tgacgccgaa aggctcgttc ctggcgacaa
     1501 ttgccggtgt tacggtaggt accctggtgt cctttgctat tacttcgctg atactgaaga
     1561 tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc aatgcggtta
     1621 aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc gcctttgttt
     1681 gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa cgcctggaaa
     1741 aagcggggct ggcaatagaa gtaaaacatt acgcgataga aaacgtgcct gcggatgccg
     1801 atatcgtcgt cactcacgca agtctggaag ggcgcgtgaa acgtgtgacg gataaaccgc
     1861 tgatattgat taataactat attggcgatc caaaactcga cactttattt aatcaattaa
     1921 ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg ctgctattta
     1981 tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg ataatgaact
     2041 gttagtgagt gtaatttctg acagcgtctg tttatcgacc tggaaagcgg cgttactcgg
     2101 tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta ccgggcatga
     2161 atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata aaaaaggcca
     2221 gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcag gctacagcta
     2281 cgaatatttt ggcggtaacg ccacctatat gattattccc gaaatcgcca ttaatttggg
     2341 ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag agcctatgtg
     2401 ctgcattatt ggtgcttatc atgccaatta tcacaccacg caatatgttt atgagcatcg
     2461 catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcgggtc cgatgggcat
     2521 tggcgctatc gattacgcca ttaacggtgg catacaaccg tcacgggtgg tggtggtcga
     2581 tatcgacgac aaacgcctgg cgcaggtgca gaagctgctg ccggtggatc tggcggccag
     2641 taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatccgg tccagacgct
     2701 gcgggcgctg acaggagatg tcgggttcga tgacattttt gtttatgcgg cggtgcctgc
     2761 tgtcgttgag atggctgacg aattactggc ggaagatggc tgtctgaact tctttgccgg
     2821 gccgacggat aaaaacttca aagtgccgtt taatttctac aacgtccatt acaacagcac
     2881 gcacgtagtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga ttgcgttaag
     2941 cgccacgggg cagttgcagc cgtcctttat ggtcacgcat atcggtgggc tggatgcggt
     3001 gccagatacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca tttataacgg
     3061 cgtgaccatg ccgctcaccg ccattgccga ttttgccgaa aaaggcaaaa ccgatccgct
     3121 gtttaaagag ctggcgcggc tggttgagga aacgcacggc atctggaatg agcaggccga
     3181 gaaatatctg ctggcgcaat ttggcgttga tattggggag gccgcgcaat gatgtccctg
     3241 gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc gcaaaccgga
     3301 tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca ggcattaaac
     3361 gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca tgcgccgatg
     3421 ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat cgcggttgac
     3481 ccgattgaag gcacgcggat ggtagcgatg gggcagagca atgcgctggc ggtcatggct
     3541 ttcgcccctc gagatagcct gttacatgcg cccgatatgt atatgaaaaa gctggttgtt
     3601 aatcgactgg cggcgggcgc gattgatctg tctttgcctc tggcggacaa cttgcgcaac
     3661 gtggcgcggg cgttaggcaa accgctggac aagctgcgca tggttacgct ggataaaccg
     3721 cgcctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt cgctctgccg
     3781 gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta cgacgtgatg
     3841 tacaccatcg gcggtgcgcc agaaggcgtg atttctgcct gtgccgttaa agcattaggt
     3901 ggggatatgc aggccgagct gattgacttt tgtcaggcta aaggggatta cacggaaaat
     3961 cggcagattg cagagcagga gcgcaagcgt tgtaaggcaa tgggcgttga cgttaaccgt
     4021 gtgtactcgc tcgatgaact ggtgaggggg aacgatatcc tctttagcgc tacgggcgtg
     4081 acgggcggcg agttggtgaa cggtatccaa cagacggcga atggggtgcg gacgcagaca
     4141 ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgca ttgatggtga
     4201 tctatcggaa gaccaatggc gacgctgaca gaagatgatg tgcttgagca actggatgca
     4261 caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca ggggatacgc
     4321 cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga tcgtcgaata tgcagtgaag
     4381 ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcactgcg tttgatttat
     4441 gcgctgggga aaatggataa atggctgtac gccgatatca cgcatttttc ccagttctgg
     4501 cattacctga acgaacagga tgaaacgccc ggatttgccg atgacatgac ctgggatttt
     4561 atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt gaaagcgatg
     4621 aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt caaaacggcg
     4681 ctgacgctgg cagtaacgac aactttaaag gaattaacgc cgtgaaaatc gaactgacgg
     4741 tgaatggact aaaggtacag gctcagtact ctgatgatga aattgagaat gttcataaac
     4801 cgctactgcg tatgctggcg gccttacagg cggtaagttc gcagcgacgt acggtggttt
     4861 ttctttgcgc tccgccgggg acgggtaaat ctaccttaac taccttctgg gaatatctcg
     4921 cgcaacaaga cccggaactg cccgctattc agacgctccc gatggatggt tttcaccatt
     4981 acaatagctg gctggacgcg catcaattgc gccccttcaa aggcgcacca gagacatttg
     5041 acgttgtgaa actggcggaa aatctgcgcc aggtcgtgga aggagattgt acgtggccgc
     5101 agtacgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc gcaccactcg
     5161 tcatcgtcga aggaaactgg ttgttgctgg acgacgagaa gtggtgccag cttgcgcaat
     5221 tttgtgattt ctcgatcttt atcaacgcgc cagctactgc gttgcgggaa cgactggtag
     5281 ggcgcaagct ggcgggaggg gtatcgctgg cagacgcaga agctttttat gaccgtaccg
     5341 acgggccgaa tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg accttaatga
     5401 tgaccgctac gggtgaatat cgtctggtgg attaacgttc ctgattagac catcttccag
     5461 tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga ttgctacgac
     5521 gctcgtgcct cgctggcggc acttctccag ccagctttca aacacgctca accaattttc
     5581 gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg cttccaggca
     5641 ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg cgttggcagt
     5701 gtctgctaac tgacaacatt gcagggcagc atgggtacgt tgagtgattt catccgccgg
     5761 gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg acggtgctgt gaaaaagttg
     5821 gttttccgct tcctgaaaca gtacgccaat ctttgccgcg cgctggcggt ttttcaggtt
     5881 tttcatcgcc tgttgttgca gcataaccgt accggctgtg ggcgtaagaa gacctgccat
     5941 tactcgtaac agcgtcgatt tccctgcgcc attgtcgccg gtcagcgcca accattcacc
     6001 ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag gccagcgata
     6061 agatatctga tttaacgtta acatagtggc tggagtgctc cgttttgcaa ttgccacgct
     6121 tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa aaagaataat cagcgaacca
     6181 cgttcaaacg cccagtgttg taagcgctgc aacagcattt cgctggcctg cggcgtcagg
     6241 cggctaaagg cttcatcaag aattaatagc tttggctgca tcgcgatggc gcaggcgatg
     6301 accacgcgct gtgtctctcc accggagagt gttgccgggt gacgatggcg caacggctgg
     6361 cactcggtca gagtcagggc ggcatcaatc cgcgccataa tctctgcttc agcaagacac
     6421 agattttctg gcccaaaggc aacttcttct tccacgctaa aggtacagcc ggagagctgc
     6481 aaatagggcg attgctggac gagctggatg gtggcggatt gttcattaag cgggagatgc
     6541 ccaatgggcg taccgaggag cgtccccgtg cctgttatct ctcctggcag aaagtccgga
     6601 taccagcccg ccatcagctg tgccagcgta cttttgccac tgccgttgtc accaaagatg
     6661 gcgaccatcc ccggtttgac atagtgaaag tcataacaga acggaggatg cgttgagtgt
     6721 gtcgggcaat aacggaactg ttctaacgta accatatcca cgctccgaat tctgtcagca
     6781 tcagcaagat catggtgtaa cgcgctacgc gttgtagggt gctatcggct ggtgcccaca
     6841 acgttgttcg tcggttgtgg atccgaaatg cccgcatatc cagcgccgca ccacggacag
     6901 caagattgtt tagcgcattg tgtgttagcg gaataattaa cgcgggcatc gcacgtaaac
     6961 gctgatacca gccctcatcc agcggcacgc cgcgggcacg ctgcgcttca tgaataatcg
     7021 ctaattgccg ttttaactgc tcaacgacca ataacggccc ggcaaacaga taagcgacgc
     7081 ctggcggcag gcgagaagca aacagagcgc gaataaaaca ttgcacgggg acgtactgca
     7141 tccacaactg tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta atagcatgcg
     7201 accaccgttc tggcgagcgt ggcgtgccgc ttagccattc tggtagccag ccaccgtgta
     7261 ccagccacag cccggctccc agcgagaaca tcagccagac gacatatttt gcccgccgcc
     7321 gcgtggcctt tagcgcaata aggcagaaaa aggtcgccgc gctatagatc ggcaaaatgg
     7381 tctgcgcggg caggataagc gtggtgcagg ccgccaatgc ccacagtgtt aatgaggtaa
     7441 atggatgcat ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca
     7501 gctggcgcag taaaagccag acaatcaccg ctgtaagaat tttatcgacc aggttcgcgc
     7561 ctatcaccgt aatcgccacc gactccacca ggttttgccc catcgagtgc atccaggcaa
     7621 cgaacaaatc ggctccgcta ccggtgacac cgccgaacag cgcagtacgc agcggtacag
     7681 cgacgaccgt taccgcaagc gtaataatca cgccgctgac aacgactttt ggcagggtgc
     7741 gaaaccaacc cgctcgcgcc aggcaaccgg cgaccaggcc gatgaccatc gcgacgggtg
     7801 caaatgcggc ggcgatcggg tcggtcagca atccccagag taaattggtc agtaagccgg
     7861 tcagcattcc gatcaccgga ccaagcagta ccgcgctaat aagtgtgccg atggagtcga
     7921 gaaatatagg taatttgacc atgctggcca gttgcccgcc gatcatattg atggcgatag
     7981 atataacgat caacaccagt gcctgactgg aaaagtggct acgcgccata agaaaacctc
     8041 tttgaaagta gttatttaac gggggaaata tgcaactcgt cgtaaccaga aagcgcgcaa
     8101 ggttggcggc taaacgccag ttcctcaagg ctaccgatca ccagttcaag cgtggtccgc
     8161 acggtacagc cgggcatcat atgaccgcca agcatcgtgc cgtgtggatc ggaaacgcag
     8221 agatgaagat gctcaccact ctgttccagc gtgccgttta gtgagatcac ttcgaatgta
     8281 ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca aggcgacgtc agtcaggcta
     8341 ccggtacagc ctgcaatcca ggcggcatga agttgatgct gctgtgcaaa agcgtgtagc
     8401 tgggaaaaaa cttcctgccc ggggaggagg cgcagtgcgt aaaagcgtgc ggaggaagaa
     8461 tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt gcggataata
     8521 tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca caacgaaaag
     8581 attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg ggcggggctg
     8641 aaaacgtgac gtaagtcaga taattgcttt ctttgactgg acaatcattc cttttattcc
     8701 acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca attaaccaat
     8761 cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta ctctgtctgg
     8821 caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag cgataaatgg
     8881 cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatccggac gccgggcgga
     8941 aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc atttgttgaa
     9001 atatgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg atcaactttt
     9061 tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc tcccctggcg
     9121 tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc gcgagcatgg
     9181 cgaagcgcat attgccgccg gggccaaaaa agtgctcttt tcacatcctg gcagtaacga
     9241 tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat
     9301 cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat tgttagatga
     9361 tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc acgatcaaca
     9421 ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc agtcgatcat
     9481 tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat ttaacgatcg
     9541 ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg atttaagcgt
     9601 gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa aagcagcaca
     9661 aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg tagattttaa
     9721 ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg gcgcacacct
     9781 gatcaaaacg ttggtctggt gcgataacga agggggcttt gctaaccgaa tgctcgacac
     9841 gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct gcaaaacttt
     9901 tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg gatcttgctg
     9961 ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg aaagtaacca
    10021 gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa caaggcgcaa
    10081 aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct
    10141 ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt cgtctggtta
    10201 aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg gaaaacgttc
    10261 gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc caaaaaatac gctgcactgt
    10321 gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct tctactcacg
    10381 gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct gaactggacg
    10441 cgctgggtaa agcactgaaa gaacctgctc gcccgatggt ggctatcgtt ggtggttcta
    10501 aagtatctac caaactgacc gttctggact ccctgtctaa aatcgctgac cagctgattg
    10561 ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg ggtaaatccc
    10621 tgtacgaagc tgacctggtt gacgaagcta aacgtctgct gaccacctgc aacatcccgg
    10681 ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac tgcaccggct accctgaaat
    10741 ctgttaacga tgtgaaagct gacgagcaga tcctggatat cggtgatgct tccgctcagg
    10801 aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt
    10861 tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc gcagacagcg
    10921 aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg ttcggcattg
    10981 ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc gtggaaggta
    11041 aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc
    11101 agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca tactctaaat
    11161 aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca taagtaagtg
    11221 actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgatga gtataaggcc
    11281 cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc tggcgtaatc
    11341 actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt cgcactgcca
    11401 gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaactgc tgctaaagtt
    11461 aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc tggtaaaggc
    11521 gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg tgcgcatcac
    11581 gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga ccactgcgcg
    11641 aagaaactgc tgccgtggat cgacggtctg ttggacgcgg gtgaaaaaca cttcgcagct
    11701 accggtaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc tctgcaagag
    11761 aacatcgaaa tctgctctaa atacctggag cgtatgtcca aaatcggcat gactctggaa
    11821 atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca catggacgct
    11881 tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgaact gagcaaaatc
    11941 agcccgcgtt tcaccatcgc tgcgtccttc ggtaacgtac acggtgttta caaaccaggt
    12001 aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc taagaaacac
    12061 aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc tactgctcag
    12121 gaaatcaaag actccgtaag ctacggcgta gtaaaaatga acatcgatac cgatacccaa
    12181 tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta tctgcagggg
    12241 cagttgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga tccgcgcgta
    12301 tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg
    12361 aacgcgatcg acgttctgta agatattcct ttctgcttat ctcaaggccc gctctgcggg
    12421 tctttttttc gccaaaagaa aaattgcctg atgcgctacg cttatcaggc ctacacgtat
    12481 cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca cgccgcatcc
    12541 ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga ttttttcgcc
    12601 aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta tgctttattg
    12661 tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca ttttgagtca
    12721 gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga
    12781 agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca
    12841 ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc
    12901 ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt
    12961 tacggtatta tcgcctttac gctaatcgct gccctgggac gcgtgggtgt acaaacagcg
    13021 tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca
    13081 ctttctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggagaa
    13141 tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt ctccaccacc
    13201 atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat tgccggaaat
    13261 attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg cgtggcgtat
    13321 gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc tgaagatcgc
    13381 attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc gtcgattaat
    13441 ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg
    13501 gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc gcaaatggat
    13561 gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag gccagataat
    13621 actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat caatttccgc
    13681 taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat atccaacgtg
    13741 ttttcttatt actttcaagg tcttgctctt ggggcagcta tgattctgcc gctcggccca
    13801 caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat gattgcctta
    13861 ctttgcgcta tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg tagcgcgtta
    13921 ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagc cttcttgctg
    13981 tggtatggtt ttggcgcttt taaaacagcg atgagtagta atattgagtt agctagtgcc
    14041 gaagtcctga agcaaggcag atggaaaatt atcgccacca tgttggcagt gacctggctg
    14101 aatccgcatg tttacctgga tacttttgtt gtactgggca gccttggcgg gcagcttgac
    14161 gtcgaaccaa aacgttggtt tgcgctcggg acaattagcg cctctttcct gtggttcttt
    14221 ggtctggcta ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa atcacagtgc
    14281 attatcaatc tggtagtggg atgtgtcatg tggtttatag ccttgcagct ggcgagagac
    14341 ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa aataaaacag
    14401 aggcgctaag cttgcctcca gaggtcctga attttgggca agtcaattaa cacggagaga
    14461 ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc gggatggcag
    14521 cgcaggctaa cgaattgccg gatggaccgc atattgtcac ctccggtacg gcaagtgtgg
    14581 atgcggtgcc agacattgcc actcttgcga ttgaagttaa cgtggccgcg aaggatgccg
    14641 ctacagccaa gaaacaggca gatgagcgcg tcgcacaata catttccttc cttgaactca
    14701 atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca gattatgatt
    14761 atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg gaagtcacgc
    14821 tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg ggtcttaacg
    14881 aaattcgttc tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac aaagcgcgta
    14941 aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc tttcatcgta
    15001 aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc agcccaatgg
    15061 tgcggatgat gaaagccgat gccgcgccgg tgtctgccca ggaaacttac gagcaggccg
    15121 ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg gatcaacaac
    15181 ccgctaaaac acctgccgca caataatact tgtagcccga cgtattcgtc gggctaatgc
    15241 ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac gtaatcacct
    15301 atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg gacaaggatg
    15361 cgggggaggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat tttattagcg
    15421 caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc gccatttggc
    15481 aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg caaagataat
    15541 aatatcagcc tcaataaagc cggacaggaa ctgtatcaaa aactgttccc ggtttatcaa
    15601 cggctttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg agaaattgtg
    15661 attggcatag ataatactta tccgacaatt attttcgatc agttgattag tctcggagat
    15721 aagtatgaag gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt tatcgataat
    15781 ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc acgagttcag
    15841 gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgggctt tctcgtttct
    15901 cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgccctg gctacaaatg
    15961 cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg accttgtggg
    16021 atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag cgccgtggag
    16081 cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa tccggcaacg
    16141 ctgaaatatt ttgatgcgcc gcacaggccg atgtatatgc aggaatatct ttattcaatc
    16201 agaaatcatc gttataccgc cacgatgctt cagcatattg ctgaagatcg tgacgggaca
    16261 aaccattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc gaagacgtgg
    16321 ctaagatcgt ggtgaatatg tccgccgggc gcattttcca gatagcgatg cagataatcc
    16381 cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag cggggaaaga
    16441 ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg ttcgctgtga
    16501 tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc aatcgacgga
    16561 gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc gatgccgttc
    16621 atcagatcga ccccggctac gtgcgttgaa tttgcatgcc cgtaaatatc aaactccagg
    16681 ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt ggaaatctcc
    16741 tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat cttgcgcagg
    16801 gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc ggtttccagt
    16861 aaatgcacca ccgattcctg tagcacttcc gaatacatca tgaacggagg aatttccggg
    16921 ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact ttgcagcggc
    16981 agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt gaccacgtta
    17041 tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc gtcgggcaag
    17101 ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg ggtaccgacg
    17161 cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc aggaatcaca
    17221 atatctgcca gttctgcaac gcgcggatcg tgatagtgat taagttcaat gatcactttc
    17281 ttcgctcgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca gactcgacca
    17341 tccggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa gaaaccgtaa
    17401 ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc gccctgattg
    17461 atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac agcatcggcg
    17521 tcagaaagta catcgtcagc ggcggcgctg attgacgccc ccgtcagcag gcgaatttga
    17581 tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt gggtagggct
    17641 ttcggcgaac ccgctggggt aaagccgcta aatgccacca tgtcgttatg ctggataatt
    17701 tctgccgctt catcggcggt catccttgtc cactgagttt ccatttttac tccttcgttc
    17761 gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg ttcatccctt
    17821 cctggtaatc ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt tcaaattcat
    17881 cggagttcat ggtgtgtgct tcgcccagta cacgcagctc ttctttgata acggcaatgg
    17941 ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct tccagttctt
    18001 ccacttccac aacatggttg aggatgccga cagccagcgc gcgctgggcg gtgattggcg
    18061 aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc aggttgtgaa
    18121 tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag gttgaggtac
    18181 tggcggcgat gatcagatcg gaactcatga tcatttcaaa tgcgccaccc caaacactac
    18241 cttccaccat cgaaatgatc ggtttcggga atttttggat catgcgggtg atttgacgca
    18301 atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga atatcgtgac
    18361 ctgcggagaa gactttggat ccactcggcg cgcgcaaaat gatacagcga atttccggcc
    18421 ggttgagatc gcttaacgcc tgcataagat catcaataaa gactttactt aaggcattaa
    18481 gtttgcggcc atagttaaac tcaacgaccg ccactttgtt gatagtgaca acgttaacat
    18541 actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa ttcactgagc
    18601 tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag cgtctggcgg
    18661 taatagcgat cgaaatcttc attcgcgaac aggtgattca gtacttcttc ttcggtctgc
    18721 ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc actggcagtt
    18781 agcgcggttt tgaagtcgat gatggcgtgc cagatctcat cgattccacg tttttccagt
    18841 gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag aatatgcagg
    18901 gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc atcgtctttg
    18961 ttgataacga tcagatcagc cacttccatc agcccttttt taatgccctg cagatcatcg
    19021 ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac ttctgtgtcc
    19081 gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgctgc ttcgcataac
    19141 agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga tggtaccggg
    19201 cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc cccgagaatg
    19261 ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt taatccctct
    19321 cgaatcaaca acatgccaaa ggcctcaaga aaggtacttt tccccgcgcc gggggtgccg
    19381 gtaacgccca gtcgcagggt gttaccgcag tacggcataa tggcatcaag cagttgcgta
    19441 cttagcgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg ggcgagtgtg
    19501 gcacgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc attaatcatg
    19561 atgctggctt atcagattca gtacgtcgcg cacactgtcg agcataggtg tacctggacc
    19621 ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggtg gaatgacgcc
    19681 accggcgact acgcagatat cttcgcgtcc ccattttttc agcgcttcga ccagttccgg
    19741 gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa cgtcattttc
    19801 tactgccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat ctacgtcgaa
    19861 accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgcc catcctggcc
    19921 cattttggcg atcagaatgc gcgagcgacg accattgtcg gcaaggaact gctccgtttg
    19981 cgcaacaatg gcatcgaact cggaggccga tttctcagat tgatgatagc tttgcgcaat
    20041 cacgccggta acacactggc ttggcaccag ataacggtcg aaagcggctt ccagcgcatc
    20101 ggaaatttca cccagggtgg cgcgaacgcg agcggcatta acagcggcag ccagcaggtt
    20161 ttcgttatgc tgtgcggcgt gagtcaggac gttcaacgcg gcggttacgg cggcattatc
    20221 acgggtggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca tcacgttgtc
    20281 gatctcaagt acatcggttt cgtcttcgtg atccagtttg tacttgttga caccaacgat
    20341 gacacgcttg ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt cgatcattcg
    20401 ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga tctgttggat
    20461 aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat aggatccggc
    20521 cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct gggtgttgcg
    20581 ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg cgttggtatg
    20641 cagtgactga gtaccgccca gcgtcgcagc cagtgcttca atggtggtgc ggataacgtt
    20701 gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg tacgcagcgc
    20761 cagtgatttc ggatcctgtg cgccaaatcc actgaccgct tcgctccata aataacgtgc
    20821 cgcacgcaac atggcgacgt tcataaacag atccatgccg ataccgaaga agaacgacag
    20881 gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga tgtactcaat
    20941 cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt cccccatgtg
    21001 gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc aggcgatgat
    21061 gtcggcgata atgcgcattg acggttttgg tgggtaaata taggtgttgc ggcagaggta
    21121 ctctttgaga atatcgtttt gaatggtgcc ggtcagttta tcaggtgtaa caccttgctc
    21181 ttctgcggcg acgatataaa acgccagtac tggtagcact gcgccattca tggtcatcga
    21241 aaccgacatt ttatccagcg ggatctggtc gaacaggact ttcatatctt ccacggtgtc
    21301 gatagcgacg cccgctttgc cgacgtcacc cgccacgcgt gggttatcgg agtcgtagcc
    21361 acggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg ccaggttacg
    21421 gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac ggatggtcca
    21481 cggttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca aaccaggaag
    21541 ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct tgatggcgat
    21601 cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc tcaattcctt
    21661 gttggcaagc tgttgccact cctgctcgtt agacattatt tgctccgtaa aacgcaatca
    21721 aaatgagggt atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa
    21781 atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat gtgattcaag
    21841 gaatacaaaa agaagtgagg ataacgcctg atatgtgcta tcaggcatta tttgatggat
    21901 taatcctgac gaaggacttt gtgaccataa tcgagcaacg catcagtgac tttacgcatc
    21961 atgcggcttt caggagcaaa gcggtgccag tagagcatcc tgcgttgaaa tagccctggc
    22021 gttaagtcaa tcagttcacc gctggctagt tctttctcga tttgcaggtg cgggatcata
    22081 cagcaggtgg tgccctggcg agcaagttgc acgaacgctt ctgaagaatt aacgatatgg
    22141 cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg gtgcatatcg
    22201 tcaagatggt caaaggcgac cacaggtgct ttcaataatg ccgaacgcgt tacgccgtta
    22261 gggaaatatt tttcggcaaa cggttttgag ctgacgaaca gatagtcaag cgcaccgagc
    22321 ttatcgacaa ggcaactcgg cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg
    22381 ccgcggcgca gacgttcctg agtacgggtt tcatcttcta cctgcaagtt gaggcggata
    22441 ggcgaatcag ccaacacagg agccagtgcc ggaagcaacc acgtcgccag actgtcggcg
    22501 ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc cagccactct
    22561 tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg ttccgtcggg
    22621 cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag ttgcttaatg
    22681 cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt
    22741 tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt
    22801 gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc aggttgcata
    22861 taccgcagcc gtcggcgggt aacatttgta tcaatggtta aacgcgcagc ttaaaagatc
    22921 agggatttgc gctattgcat ttcgcttagc gacttcgcgc cctggttatg ttgaatggcg
    22981 tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg tatgctttat
    23041 ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag ttaaccacgc
    23101 ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa atttcatacc
    23161 acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg ggcggcactt
    23221 cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc cgcacacttt
    23281 attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag ttcagatgct
    23341 tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga agtcgacagc
    23401 cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat gatcaaaggc
    23461 ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa atttatctgt
    23521 attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgtc agtagaagtt
    23581 atcccgatgg cacgtagtgc agtggcgcgt cagctggtga aactgggcgg tcgtccggaa
    23641 taccgtcagg gcgtggtgac cgataatggc aacgtgatcc tcgacgtcca cggcatggaa
    23701 atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt ggtgactgtt
    23761 ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga cggtgtcaaa
    23821 accattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct cttcattaaa
    23881 tttggtgaca tgtgtcacgc ttttaccagg caattgtcga ttgctctaaa taaatcctct
    23941 aaaccagcat attcatccaa gaattacctt tgcgtgatat ttcctcaaca tcgcgacgca
    24001 aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggatg caaatccgca
    24061 cacaacattt caaaagacag gattgggtaa atggcaaagg tatcgctgga gaaagacaag
    24121 attaagtttc tgctggtaga aggcgtgcac caaaaggcgc tggaaagcct tcgtgcagct
    24181 ggttacacca acatcgaatt tcacaaaggc gcgctggatg atgaacaatt aaaagaatcc
    24241 atccgcgatg cccacttcat cggcctgcga tcccgtaccc atctgactga agacgtgatc
    24301 aacgccgcag aaaaactggt cgctattggc tgtttctgta tcggaacaaa ccaggttgat
    24361 ctggatgcgg cggcaaagcg cgggatcccg gtatttaacg caccgttctc aaatacgcgc
    24421 tctgttgcgg agctggtgat tggcgaactg ctgctgctat tgcgcggcgt gccggaagcc
    24481 aatgctaaag cgcaccgtgg cgtgtggaac aaactggcgg cgggttcttt tgaagcgcgc
    24541 ggcaaaaagc tgggtatcat cggctacggt catattggta cgcaattggg cattctggct
    24601 gaatcgctgg gaatgtatgt ttacttttat gatattgaaa acaaactgcc gctgggcaac
    24661 gccactcagg tacagcatct ttctgacctg ctgaatatga gcgatgtggt gagtctgcat
    24721 gtaccagaga atccgtccac caaaaatatg atgggcgcga aagagatttc gctaatgaag
    24781 cccggctcgc tgctgattaa tgcctcgcgc ggtactgtgg tggatattcc ggcgctgtgt
    24841 gatgcgctgg cgagcaaaca tctggcgggg gcggcaatcg acgtattccc gacggaaccg
    24901 gcgaccaata gcgatccatt tacctctcca ctgtgtgaat tcgacaacgt ccttctgacg
    24961 ccacacattg gcggttcgac tcaggaagcg caggagaata tcggcctgga agttgcgggt
    25021 aaattgatca agtattctga caatggctca acgctctctg cggtgaactt cccggaagta
    25081 tcgctgccac tgcatggtgg tcgtcgtctg atgcacatcc acgaaaaccg tccgggcgtg
    25141 ctaactgcgc tgaacaaaat cttcgccgag cagggcgtca acatcgccgc gcaatatcta
    25201 caaacttccg cccagatggg ttatgtagtt attgatattg aagctgacga agacgttgcc
    25261 gaaaaagcgc tgcaggcaat gaaagctatt ccgggtacca ttcgcgcccg tctgctgtac
    25321 taattcccct tctctgaaaa ttaacgggca ggtcactgac ttgcccgttt ttttatccct
    25381 tctccacacc gttgccgcgt tgcgcattct actttgcgag tctgctccag aaaataaatc
    25441 aaagtcagat caatgcgttg tgtttattta atggggattt acgatggcgg gacagcatgg
    25501 ggctgtaacg gtaaagccct caccgaagcg agggcttaaa ggagaagggt tatgatgcga
    25561 cttgtcatca tactgattgt actgttactg ataagtttcg gagcttatta acagtcagtc
    25621 tcaggggagg agtcatcctc ccttaccctt actcactaaa ttaggtcaaa gaatcaacga
    25681 tgtcaatcag ggcgatgcgg ggtgcatcgc cctgaaccga actttttgat agccacttca
    25741 tcgcccacgt ttactgccgt aatagatcct ttttcccaaa aattatctct gttttgcatg
    25801 gggtttttcg agcacgctcg aagaaaagat ttaaaggtag atcaatgcgt tgtggttatt
    25861 taaagagagc ttacgatagc tgggcagcat ggggctgtaa cggtaaagcc ctcaccgaag
    25921 cgagggcttg aaggagaagg gttatgatgc gacttgccat catactgatt gtactgttac
    25981 tcataagttt cccagcttat taacagtcag tcacagggga ggagcaatcc tcccttaccc
    26041 ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg
    26101 cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga tatcccactc
    26161 ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg gttgcgtttt
    26221 atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc ccaggcgctg
    26281 accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg aaaggggtag
    26341 cacgtcacgc acatccagtt ttggctcatg gatcttcaac ctgttcatca ccagttcgct
    26401 ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca aaactggaag
    26461 atatacgcgc ttaccggcgc gccagagttg ttctattagc ggctgggtgt cgagttcacc
    26521 atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat aagtcatcat
    26581 ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg cacgacgacg
    26641 ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt ctggtagttg
    26701 cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag gctgtaaccc
    26761 ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc ggacggaccg
    26821 agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg gctcagggga
    26881 ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct accacagtaa
    26941 accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc ttgttcaagt
    27001 aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact tgccgcgtag
    27061 tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc ggcaatgaag
    27121 accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa ccgttggttc
    27181 agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt cacacgcagt
    27241 gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc ctgctgattg
    27301 actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta tagctaccct
    27361 gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag catatcatga
    27421 atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc ctggttacaa
    27481 cgaaatgaac cagtatctga accaacaagg gacgggtctg acccctgctg agatgcatgg
    27541 tttaatcagt gggatgatat gtggcggtaa cgatgacagc tcatggctac cgctacttca
    27601 cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat
    27661 gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc tttatctgcc
    27721 tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt gggtcaatca
    27781 cttcctgctt ggtcttggcg ttacgcaacc gaagctggat aaagtgaccg gcgaaaccgg
    27841 tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag acgaagatca
    27901 ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt
    27961 atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa aaccgactct
    28021 acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg caagagtttc
    28081 agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg
    28141 ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag aacagtgact
    28201 tctggtactt caccggcttt aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg
    28261 acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg gagatctggt
    28321 ttggccgtcg cttaggccag gatgccgcgc cagagaaact gggcgttgac cgcgcactgg
    28381 cattcagcga aatcaatcag caactttatc aactacttaa cggcctggat gtggtttacc
    28441 atgcccaggg cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc
    28501 gtaaaggttc gcggcaaaat ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg
    28561 ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtactccgc cgcgcgggag
    28621 aaatcaccgc catggcacat acccgggcga tggaaaaatg ccgtccggga atgttcgagt
    28681 accatctgga aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgc tatccgtcct
    28741 ataacaccat tgtcggcagc ggtgaaaacg gctgcattct gcactacacc gaaaacgagt
    28801 gtgaaatgcg cgacggcgac ctggtgttga ttgacgcggg ttgcgaatac aaaggttacg
    28861 ctggcgatat tacccgcacc ttcccggtca acggcaaatt cactcaggcc cagcgtgaaa
    28921 tctacgacat tgtgctggag tctctcgaaa ccagcttgcg cctgtatcgt ccgggaactt
    28981 ccattctgga agtcactggt gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg
    29041 gcattctgaa aggtgatgtt gatgaactga tcgctcagaa cgcccatcgt cctttcttta
    29101 tgcatggcct tagccactgg ttagggctag atgtccatga cgttggcgtt tatggtcagg
    29161 atcgctcgcg cattctggaa ccggggatgg tactgaccgt agagcctggt ctgtatatcg
    29221 cgccggatgc ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt gaagacgaca
    29281 ttgtgattac cgaaaccggc aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag
    29341 aaatcgaagc gttgatggca gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg
    29401 catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacggga cgctgccggt
    29461 acatttgatt gaagcgaccg cgccagagtc acatgctcat ccgggctttg atggacgagc
    29521 tattgcgctg gctgcgggga cctgtcagca actggcgcgc atcggcgtct ggcaatctct
    29581 ggcggattac gcaaccgcca tcactaccgt gcatgtcagc gatcgtggtc acgctggatt
    29641 tgtcaccctc gccgcagaag attaccaact ggcggcgctg ggacaggttg tcgaattgca
    29701 caatgtcggg caacggctgt ttgcattgct gcgtaaagca cctggcgtaa cgctgcattg
    29761 ccctgatcgc gtggctaacg ttgcccgtac tcagagtcac gttgaagtga cgctggagag
    29821 tggcgagacg ctgacgggcc gcgtgctggt agcagctgat ggcacccatt cagcgttagc
    29881 caccgcgtgc ggcgttgact ggcagcagga gccttacgaa caactggccg tgattgccaa
    29941 cgttgcgact tccgttgcgc acgaaggccg cgcttttgaa cgcttcacgc aacatggccc
    30001 gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc atccactgga
    30061 acggcgcgaa gaggtgctgt cgtggagcga cgagaagttt tgccgtgaac tccagtcggc
    30121 ctttggctgg cgacttggaa aaattaccca cgccggtaaa cgcagtgctt atccgctggc
    30181 gttaacccgc gccgccagag ctattaccca tcgtaccgtg ctggtgggca atgcggcgca
    30241 aactctgcac ccgattgccg ggcaagggtt taacctcggt atgcgagatg tcatgagcct
    30301 tgcggaaacc ctgactcagg cgcatgagcg cggagaagac atcggtgatt acggcatatt
    30361 gtgccgctat cagcagcgtc gacagagtga tcgcgaagca accatcggcg tcacggacag
    30421 cctggtacat ctttttgcca accgttgggc accgctggtt gtcgggcgca acatcggact
    30481 gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg
    30541 ggtggcgcgt tgaaggcggt tttaaggaat aaacaatgca aagtgttgat gtagccattg
    30601 ttggtggcgg catggtgggg ctggcggttg cctgcggctt acaggggagc ggcttacgcg
    30661 ttgccgtact ggagcagcgc gtaccggaac ctctggcggc ggatgcacca ccacaactgc
    30721 gcgtttcggc tatcaatgcc gccagcgaaa aattactcac ccgtcttggc gtctggcagg
    30781 acattctctc tcgtagggcc agttgttatc acggtatgga agtgtgggac aaagacagtt
    30841 ttggtcacat ttcgtttgac gatcaaagca tgggctatag ccatcttggg catatcgttg
    30901 aaaactcagt gattcactac gcgctgtgga acaaagcgca gcagtcgtca gatatcactc
    30961 tgttggcccc cgcagaatta cagcaggtcg cctggggaga aaatgaaact ttcctgacgc
    31021 tgaaagatgg cagcatgtta acggcgcgtc tggtgattgg cgcggacggt gctaattcct
    31081 ggttgcgcaa caaagcagat attccgctga ctttctggga ttatcagcat cacgcgctgg
    31141 tagcgaccat tcgcacggaa gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg
    31201 aaggcattct ggccttttta ccgcttagcg atccgcatct ttgctcaatt gtctggtcac
    31261 tgtcgccaga ggaagcgcag cggatgcagc aggcaagtga agatgaattt aatcgcgcgt
    31321 taaatatcgc ttttgataat cgcctgggct tatgcaaggt tgagagcgcg cgtcaggtgt
    31381 tcccactgac ggggcgttat gcgcgccagt ttgccgcgca ccgtctggtg ctggtgggcg
    31441 acgccgcgca taccattcac ccgctggcgg ggcagggagt aaatctcggc tttatggatg
    31501 ctgcagagct gattgctgaa ctgaaacggt tgcatcgtca ggggaaagac atcgggcagt
    31561 acatttacct gcgccggtat gagcgtagcc gcaagcacag tgcggcgctg atgctggctg
    31621 gtatgcaggg attccgcgat ctgttttccg gtaccaatcc ggcgaaaaaa ctgctgcgtg
    31681 atattggttt gaaactggcc gacacgcttc ctggcgttaa accacaactt atccgccagg
    31741 caatgggatt aaacgatttg cctgaatggc tgcgttaaaa atttctcttc tgctgtttat
    31801 ttgataccca tcacactttc atctcccggt tttttcgccg ggagattttc ctcatttgaa
    31861 ataaactaat ttcacctccg ttttcgcatt atattttcta atgtcattat tttttgattt
    31921 agtgtttttt gacatttttt tagctcttaa tattgcctta ttcaaattag ctttctaatg
    31981 gcatcatctt tatatagaaa ctggtgtgtt ttttggtttt ttattctgtc gcgattttgg
    32041 cgttttttaa ccataagcta atgtgatgat caattttacc ttatggttaa cagtctgttt
    32101 cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg accgggagtg gctccgatgc
    32161 tgggtttcgt ggtgataatt tcaccatgaa aagttgtcag ccccgcttat tcaatgagga
    32221 caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt
    32281 ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc
    32341 ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg accatcgtcg atctccgcgg
    32401 cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa
    32461 aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgacct
    32521 catcgtctac tactttactg aagatttctt ccgcctcgtt gttaactccg ccacccgcga
    32581 aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg
    32641 tgatgacctt tccatgattg ccgtgcaagg gccgaatgcg caggcaaaag ctgccacact
    32701 gtttaatgac gcccagcgtc aggcggtaga agggatgaaa ccgttctttg gcgtgcaggc
    32761 gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg aaattgcgct
    32821 gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagcgggcg ttaagccatg
    32881 cggtctgggc gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt atggtcagga
    32941 gatggacgaa accatctctc cgttagccgc caacatgggc tggactatcg cctgggaacc
    33001 ggcagatcgt gacttcatcg gtcgtgaagc cctggaagtg cagcgtgagc atggcacaga
    33061 aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg
    33121 ttttaccgat gcgcagggca accagcatga aggcattatc accagcggga ctttctcccc
    33181 gacgctgggt tacagcattg cgctggcacg cgtgccggaa ggtattggcg aaacggcgat
    33241 tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa
    33301 cggtaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca
    33361 gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttacacc
    33421 gttggtataa ccgaacatgc ccaggagctg ttaggcgata tggtgtttgt tgacctgccg
    33481 gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc agtaaaagcg
    33541 gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc
    33601 gattccccgg aactggtgaa cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa
    33661 gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta
    33721 gaagacgagt aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaaattta
    33781 tgcctcaccc tcactctctt cgtaaggaga gaggttcaca attcactgca cgtttcagga
    33841 accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt ttattgaacg
    33901 ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg gtgcacaatc
    33961 gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga ccccgccgca
    34021 ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta ttgccagtcg
    34081 caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc taccgccggt
    34141 tatcctgagt aacatgctgg aaaatccggg ctggtatacc gcgtacactc cgtatcaacc
    34201 tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa cgctggattt
    34261 gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg ccgccgaagc
    34321 aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc
    34381 cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga
    34441 agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt
    34501 acagcaggta ggcactaccg gtgaaattca cgactacact gcgcttatta gcgaactgaa
    34561 atcacgcaaa attgtggtca gcgttgccgc cgatattatg gcgctggtgc tgttaactgc
    34621 gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg gcgtgccgat
    34681 gggctacggt ggcccacacg cggcattctt tgcggcgaaa gatgaataca aacgctcaat
    34741 gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc
    34801 gatgcagact cgcgagcaac atatccgccg tgagaaagcg aactccaaca tttgtacttc
    34861 ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc cggttggcct
    34921 gaaacgtatc gctaaccgca ttcaccgttt gaccgatatc ctggcggcag gcctgcaaca
    34981 aaaaggtctg aagctgcgcc atgcgcacta tttcgacact ctgtgtgtgg aagtggccga
    35041 caaggcgggc gtactggcgc gtgccgaagc ggctgaaatc aacctgcgta gcgatatcct
    35101 gaacgcggtt gggatcaccc ttgatgaaac caccacgcgt gaaaacgtaa tgcagctttt
    35161 cagcgtgctg ctgggcgata accacgggct ggagatcgac acgctggaca aagacgtggc
    35221 tcacgacagc cgctctattc agcctgcgat gctgcgcgac gacgaaatcc tcactcatcc
    35281 ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg
    35341 taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaactgaa
    35401 cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc acccgttttg
    35461 cccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg actggctggt
    35521 gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac agggcgaata
    35581 cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat
    35641 ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata tggcaggaat
    35701 gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg atctgcgcgc
    35761 gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc cttctaccca
    35821 cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca
    35881 ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat
    35941 tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc acggcggtgg
    36001 tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca
    36061 tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt
    36121 cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg
    36181 gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca
    36241 ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct
    36301 cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct
    36361 gatcgactac ggtttccacg cgccaacgat gtcgttcccg gtggcgggta cgctgatggt
    36421 tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc
    36481 tatccgcgca gaaattgacc aggtgaaagc cggtgtctgg ccgctggaag ataacccgct
    36541 ggtgaacgcg ccgcacattc agagcgaact ggtcgccgag tgggcgcatc cgtacagccg
    36601 tgaagttgcg gtattcccgg caggcgtggc agacaaatac tggccgacgg tgaaacgtct
    36661 ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca
    36721 gtaattcact gattcgacta ttttctaaag gcgcttcggc gcctttttag tcagatgaca
    36781 aagtacaaaa gtgctcagac agtcccctcg cccctttggg gagagggtga ggggaacagg
    36841 ccggcactgg cgcgaatatt taccctcacc ccggccctct ccctgaaagg gcgaggggga
    36901 aaagcgtgcc aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg
    36961 aatgaatttt tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc
    37021 atcgggcggg caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat
    37081 caacaaaacc tccacgcggc gcaggaagtg atgaacttaa taacgcaagc cggtggcaaa
    37141 gcattcgtgc tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca
    37201 atcgatcagc acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc
    37261 cagtgcaccg ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg
    37321 acgggatatt ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc
    37381 agtggcggcg ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa
    37441 tatgttgatt acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg
    37501 gaagtcgccg cgcaggggat ccgggttaac tgcgtgcggc caggggttat ttataccgaa
    37561 atgcacgcca gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg
    37621 cagcgtggtg gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa
    37681 gcctcttacg tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt
    37741 gtctgaccgg atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg
    37801 ttgctggcaa tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg
    37861 ccagtaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg
    37921 gtagtgaatg acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca
    37981 tcgtaagtca ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag
    38041 tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca
    38101 ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact
    38161 ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca
    38221 aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt catgtaatag
    38281 ctgaaaccaa gataatcgca ggtgccttca cgcagtacat cgagatcgcc gtcttccatt
    38341 ttgatgttaa atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt
    38401 agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg
    38461 tccgggttac agcagtaggg atagagcggc accatcgcca gcatacagcc gactttcatc
    38521 tccgggttaa tgcgacgcgc agctttcacc gccagggcgc tggcgacaaa ctggtgatgc
    38581 agcacctgat acatcgtctc ttccgggttc tcatgctcgg tataaaccac gccggagcag
    38641 cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt gaaggtcatc
    38701 cagtatttga ctttatgctt atagcgttca aacaccactt ccgcgaaacg gacaaagaaa
    38761 tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag cggcatttca
    38821 aagtgggaga gggtgatcac cggttcgatg ttgtatttca gcagttcatc gaacatatca
    38881 tcgtagaact tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc
    38941 gtccaggcga tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct
    39001 tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt gcctggcaag
    39061 acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg
    39121 ctcggccctt tgccgccttt gttccagccg ccttcgacct gatgagcggc aactgcgccg
    39181 ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc
    39241 aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc aacgtttcca
    39301 ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt
    39361 caaaacgggt gatgtgcttc gtgtcgggcg ttttgaagat gacggttatt tttgcacgat
    39421 tgaagtcacc gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca
    39481 ggaaaatatg accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac
    39541 acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa
    39601 tttcgcattt aatttcatga tttttaggag attagtctca aatcagaatt tattttagct
    39661 aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg
    39721 tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag
    39781 aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg
    39841 tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca
    39901 gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc
    39961 ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga
    40021 ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt
    40081 tgatgattgt tatctggagc ctggcattgc tgggtattct gtttaaactg accatcgcgc
    40141 accgattcaa aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg
    40201 taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg
    40261 gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg
    40321 ccatctggca cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt
    40381 atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca
    40441 tcaggcatcg gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct
    40501 cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat
    40561 caggcgataa atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc
    40621 tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg
    40681 ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc
    40741 gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc
    40801 agtcggctgg cgctacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt
    40861 gctcgcgcca ccatctcttg tccggtatag cagccttttt taaagctgat gccgcccagc
    40921 gcctggaggt tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg
    40981 aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg
    41041 cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct
    41101 gccgggtgtt caaaccatag caaagtggtc gcgccttctt taactacctg tttttctttc
    41161 gaaggcagtt cactaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca
    41221 agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc
    41281 agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg
    41341 tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc
    41401 aggtgctgat cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt
    41461 tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc
    41521 ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag
    41581 tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa
    41641 gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagccatt
    41701 gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa
    41761 ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga agaagatgga
    41821 cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat
    41881 ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat
    41941 ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg
    42001 taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga
    42061 acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc
    42121 tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc agttacaccc
    42181 cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc cagcggcgta
    42241 ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc
    42301 aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac
    42361 gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg
    42421 aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc
    42481 aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg
    42541 atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc
    42601 agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca
    42661 tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc
    42721 agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac
    42781 agcgcaacaa ttttaccttc gaggttcagg tcgtcgagct gatcccagac ggcttcccag
    42841 tcttcctgga tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc
    42901 tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata
    42961 atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga
    43021 cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga
    43081 atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc
    43141 aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa
    43201 atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt
    43261 tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg
    43321 cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc
    43381 gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc
    43441 tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggttgaac
    43501 gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg
    43561 aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata
    43621 tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtac
    43681 cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc
    43741 tgctgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc
    43801 gacagacctt ctggcaccgc attaaacatt atgctgtgct ggcgggtatc gacagcgaaa
    43861 agctgtcacc gcatgtgttg cgtcacgctt ttgccaccca tttattaaat catggtgcgg
    43921 atttacgcgt ggtgcagatg ctactgggcc acagcgacct ctccaccacg caaatttata
    43981 cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat
    44041 gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg
    44101 cgttttcagg ttttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg
    44161 gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta
    44221 acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg
    44281 acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg
    44341 cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt
    44401 ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg
    44461 cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag
    44521 agaaagaaat gaaagctatc tggtgtgcaa aagataaaaa caaagcgttt gatgatgtga
    44581 tggcaggtaa aagcgccgca ccagccagct gcgacgtgga tattgccgac cattacgcac
    44641 ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg
    44701 ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga
    44761 ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga
    44821 aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg
    44881 cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact
    44941 gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat
    45001 tattgtggtc ggcgattttg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc
    45061 gatgcgctcg cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg
    45121 ttacggctta agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt
    45181 cacggtggat aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat
    45241 tccggttatt gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat
    45301 cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt
    45361 ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga
    45421 gcgtggcatc gcaattctta acctggcaga actgctggat ctggtagcgc tggggacagt
    45481 agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg
    45541 catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg caaatcgcga
    45601 tgcgcaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc
    45661 cgggcgactg gacgatatgt ccgtcggtgt ggcgctcttg ctgtgcgaca acatcggcga
    45721 agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca
    45781 aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gcgcgaagtc gcgacacgct
    45841 acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc
    45901 ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg
    45961 tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg cactggagcg
    46021 attagacaca ctctaccctg gcatgatgct gaagtttggc ggtcatgcga tggcggcggg
    46081 tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac
    46141 tgagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc
    46201 ggccgaaatg accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat
    46261 gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga
    46321 acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc
    46381 ttttaatgtc gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta
    46441 caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat
    46501 ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct
    46561 cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc
    46621 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg
    46681 actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg
    46741 tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg
    46801 ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac
    46861 tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgtcc
    46921 tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg
    46981 ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga
    47041 gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca
    47101 tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg
    47161 attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc
    47221 cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt tacccggaag
    47281 ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg
    47341 cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca
    47401 tcccgaccgg gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagacc
    47461 aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg
    47521 agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt
    47581 atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc
    47641 aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat
    47701 gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca
    47761 atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg attgccttcc
    47821 cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag
    47881 agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc
    47941 gtcgtattat gggtaaagcg tctttcgtta ccctgcagga tgtggggggc cgcattcagc
    48001 tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg
    48061 acctcggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt
    48121 ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat
    48181 tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatttggat ctcatctcta
    48241 acgatgaatc ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt
    48301 tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atccctggcg
    48361 gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc
    48421 gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg
    48481 aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca
    48541 tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc
    48601 tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga
    48661 cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc
    48721 gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat
    48781 ctatcggtat ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg
    48841 aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag
    48901 tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct
    48961 tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg
    49021 cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct
    49081 acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga
    49141 ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc
    49201 tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac cccggcaaat
    49261 gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc ttcactattg
    49321 gctgcctgca tcaccatcca cggactgaac gcccacggtg tggcatcaat accgtgtaat
    49381 acatctgcta aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca
    49441 ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg
    49501 gtggcgcgat agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc
    49561 tcataacggc aacggcggct caccgcgtct tcgttgcttt ctcccagttg tgggtgccca
    49621 caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact
    49681 aataattgtc ctttggcatt aaacagccaa ctggagaacg cgagatgtaa gcgggtgtct
    49741 gccgtgtgtg cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa
    49801 atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat
    49861 aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg
    49921 gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa
    49981 tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac
    50041 tgaatatagc gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg
    50101 cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc
    50161 ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta
    50221 agggttgcga gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa
    50281 aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag
    50341 aggttgtaac ggttggtggt gtagttacag cgcgacaaaa ttcgaatccc tgtcgccagt
    50401 accatgccga acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc
    50461 agcaccgcca tttttggcac catgccaaac aggattaata taattcccga agaaatacac
    50521 acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga aaatgacgtg
    50581 tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac acgcagcctg
    50641 cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc
    50701 cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga
    50761 tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga agcatcatgt
    50821 agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc
    50881 agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa
    50941 attaagacag caaaggagat acctaaataa acgggattac catattgcgg attcccttta
    51001 cctccggcgg cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc
    51061 acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat
    51121 gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg
    51181 ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc
    51241 aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag
    51301 caaaagagat ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca
    51361 ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg
    51421 agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc
    51481 ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc
    51541 cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc
    51601 cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgcg
    51661 cttaaaccac gaaaatgaca agagaacctg acaatggaat tatctgtaat tttctcatat
    51721 tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga
    51781 tagcgctctt ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata
    51841 atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
    51901 tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata
    51961 agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa attgcctgtg
    52021 cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga ccgcgtatcc
    52081 acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc aacaatgccc
    52141 cttgtgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc gtacaactgg
    52201 acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg
    52261 agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt tccggcacgc
    52321 aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat aaagggttac
    52381 agtggataaa ggtggctcgc cagcgcaaaa cggcagcagg aaaagcgtca tcagacgctc
    52441 agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca gataaaattt
    52501 cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat ccacaacaag
    52561 cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc aactggcatt
    52621 gcccgctgca taacgatatt ccggattaca tccgtctggt acaggaagga aagattattg
    52681 aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc agggtatgtc
    52741 cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc gcagtctcta
    52801 tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg cgtcccgatg
    52861 tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctggg ccagcagggt
    52921 tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt gatcgccatc
    52981 cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat aaaacggtat
    53041 taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt aactgtgaaa
    53101 ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt ttcatcggcg
    53161 tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc ggtgtcattc
    53221 aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg gagtctgaag
    53281 agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt ggcgatacga
    53341 caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc tgcgcgtatc
    53401 gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg cgcgaggaag
    53461 gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa gatgggcgtt
    53521 taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat ggtcgccgtc
    53581 gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg attatggcct
    53641 ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa ctcgataaat
    53701 ggggcctgat tcaaaacggt gacgtcgggt atttacctac ccagacgcat ctgaaaaaag
    53761 tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca atggccgcag
    53821 gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg tgatgaaatc
    53881 gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag tagcctgtgt
    53941 ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc ggctgaaggt
    54001 acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg
    54061 tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc agaccaattc
    54121 tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat
    54181 tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct gtgaacagcg
    54241 ggaagagggg ccagcctgtg ttgaatcttg cccgacacag gcgctgcagt tgcttactga
    54301 aagagaactc aggcgagtcc gcctgcagcg tattgttgcc agcggtgaga atcctctgtg
    54361 aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac cacccgcgta
    54421 aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcaactttt gccattcgcg
    54481 agcaaagaca cggcctacga ccagtgtcac aaagcccagc ataataccgg taacgatatt
    54541 acaagtcaga acgatgaaca cggcacacac caggccagcc attgcgtcaa taaaatcgtt
    54601 gaaatccagc ttcgagacgt tacttaacat cagcaaaccc acgtacatca gagcgggtgc
    54661 agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca gcaggaataa
    54721 cgcccccact acggttgcgg tcaaccctgt tttacccccg gcggcggttc ctgccgctga
    54781 ttcgatataa accgctgcgg gcgctgcgcc caccagaccg gagaatattg aacttactga
    54841 gtcactggtc agggctttgc cgccattgat gatctggttg tctttatcca acaaattcgc
    54901 ctgaccggcg acggcacgga tggtgccagt ggcgtcgaac actgcggtca tcactaatgc
    54961 cagtacactc ggaagtacgg ttggctggag tgcgcccata atatcgaggc tgaaaatcag
    55021 agacttacca tcttcgccag tcagacttgg catcgccacc agaccgtggt atttcaccgc
    55081 tgggtcaaag attaagccga taatcgaaat tgcgataatc accaacaaga tcccgccggg
    55141 tacgcggcac ttctccagac cgaagatgac cgccagcccc agcaagctca tcatcaccgg
    55201 gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt tgataaccat
    55261 acccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac ctgtaccgtg
    55321 agcgataccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta cggaaatagc
    55381 ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc taatttgttg
    55441 cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac caatcgccat
    55501 tggcaaattg gcccacaacc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc
    55561 aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac
    55621 ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact
    55681 gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg
    55741 tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat
    55801 tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg
    55861 cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc
    55921 tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt gccaggtaaa
    55981 aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa
    56041 aacgcttcat atgccgagag gcgatagcct tgtaattgca atactttgta ggcttcgttc
    56101 agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat gcccacttta
    56161 actttcttct gccatgtttt tttcaagttg aataagccgc tgccgaggta aaggttggag
    56221 gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc tttttcttcg
    56281 agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg atggtaaaca
    56341 tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc atctttgttt
    56401 tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg ttgcgccatc
    56461 gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata tagcagacga
    56521 ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct ttcggcagtg
    56581 tcgagcagat aatccggtgc gttgcggtcc atcatcacct taccggcaat catacgcata
    56641 ttgatatgac tggcggcttc aaacagcgca tcaacagatt gcggatgaac agtgccaaac
    56701 accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaatgc cgacatttcg
    56761 cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt attcaaccac
    56821 tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg gatatgtgta
    56881 tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat agtgtcagga
    56941 atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg tttaatgagt
    57001 aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt atcgaccgta
    57061 cgggtgacat caataaaact gcctcgtacc gctttcaacg tgtgttctcc tgacatcatt
    57121 gactccttta ctgttaatcc atctcttccg ctgaggtaat acaggcagaa cgttttcctg
    57181 tcggtagcca ccagggagga taatatttaa taaaatcgca gttaacccgc cagcacaaat
    57241 agggttttca actagtacat aaatagaggc tggcaatatt ttaaaaattt cgggatcgta
    57301 ggagacgcca agtcctaaac ctaaagaagt ggcgacaata agtgtttctc ggcgctttaa
    57361 accgttggtg atgattatgc gaatcccagc gatggcaatc atggaaaaca tcaacgtcat
    57421 tgcgcctccc agaactgccg agggaatggt cgtgaagaag ccgccaatca tcggaaacaa
    57481 accgaggata accagcatta ccgcgatggt tcgcccgaca taacgtgaag cgacgccagt
    57541 catctgaata accccattat tttgcgcaaa cgtggttaag ggtaatgacc cgacagcgga
    57601 ggcgataaca gaaaccagac cgtctgccag cacgccgcct ttcagtcggg actgatactc
    57661 ttctccctga atgggtcggc gggaaaccat tgccgtggcg gtgatatcgc cgacagcttc
    57721 cagcacgcta agcagataaa tcgtgccaac caccaggaac tgatggaagc taaaactaaa
    57781 gccgtatttg aacggatgcg ggatggtgat taacggcaaa ttgcgcatac tgctgaaatc
    57841 caccatgccc aggcataacg atgcgatata gccgacacat agcccaatgg cgatccctcc
    57901 catgcgtagc aacggactgc gacagcagtt aaagccgatc accacaatta aaaccaataa
    57961 accaacgccg agatgttcgt aattgccgaa cgtaccgctg cttttggctg caaatcctcc
    58021 accaaaatcg ataatgccga ctttaatcag gcttaagccg atcatcagta ccacaatacc
    58081 gctgacggta ggcgtaataa cccgacgtaa atagggcaag ataaatgaag atccgacaac
    58141 cagaaatgcg ccaacgaagg agacgccgag aagcgacgac atgattaact cttcgtgaaa
    58201 accgtcgctt ttcatgctgc tgcctagcgc aatcatgacc gtaacaaatg aaaaattgac
    58261 tgactgaatt gagagtaggc cagaaccgac gatgccgtag cggtttactt gtaaccaggt
    58321 accaatacca gaggcgatca tcgccataga aacaagatag gcagttgttt cagcggaaag
    58381 ctgtaaggcc gcccccacga ttaacgcggg ggttaccatc ggaacgaaaa ttgccaacag
    58441 atgggtaatg gcaccaacga gagcctgatg aaagggaggg cgatcctcca gttcaaatat
    58501 aaggtcagaa cctgcattgt ttatatcaga catcccttca ctccttttaa agtaaatttg
    58561 atcatctgaa ttagtaaaat aaattcccac gaatttgggg cgcaggacgc cttgagaacg
    58621 gcttatctca ttttcgcagt
//
LOCUS       DAZFAH010000008        57190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFAH010000008 DAZFAH010000000
VERSION     DAZFAH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.008"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(98..1048)
                     /gene="corA"
                     /locus_tag="ACHJWB_000445"
     CDS             complement(98..1048)
                     /gene="corA"
                     /locus_tag="ACHJWB_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4312133.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1200..1256)
                     /gene="ysgD"
                     /locus_tag="ACHJWB_000446"
     CDS             complement(1200..1256)
                     /gene="ysgD"
                     /locus_tag="ACHJWB_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4312134.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1418..2182
                     /gene="yigE"
                     /locus_tag="ACHJWB_000447"
     CDS             1418..2182
                     /gene="yigE"
                     /locus_tag="ACHJWB_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO4312135.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGL
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(2329..4491)
                     /gene="uvrD"
                     /locus_tag="ACHJWB_000448"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2329..4491)
                     /gene="uvrD"
                     /locus_tag="ACHJWB_000448"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4312136.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(4575..5291)
                     /gene="yigB"
                     /locus_tag="ACHJWB_000449"
     CDS             complement(4575..5291)
                     /gene="yigB"
                     /locus_tag="ACHJWB_000449"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4312137.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5291..6187)
                     /gene="xerC"
                     /locus_tag="ACHJWB_000450"
     CDS             complement(5291..6187)
                     /gene="xerC"
                     /locus_tag="ACHJWB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4312138.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6184..6891)
                     /gene="yigA"
                     /locus_tag="ACHJWB_000451"
     CDS             complement(6184..6891)
                     /gene="yigA"
                     /locus_tag="ACHJWB_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO4312139.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6888..7712)
                     /gene="dapF"
                     /locus_tag="ACHJWB_000452"
     CDS             complement(6888..7712)
                     /gene="dapF"
                     /locus_tag="ACHJWB_000452"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4312140.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(7749..7952)
                     /gene="yifL"
                     /locus_tag="ACHJWB_000453"
     CDS             complement(7749..7952)
                     /gene="yifL"
                     /locus_tag="ACHJWB_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4312141.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPSADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8302..8856
                     /locus_tag="ACHJWB_000454"
     CDS             8302..8856
                     /locus_tag="ACHJWB_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO4312142.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LDMTHEQYLKGMRLEKRNKTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            9228..10670
                     /locus_tag="ACHJWB_000455"
     CDS             9228..10670
                     /locus_tag="ACHJWB_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4312143.1"
                     /translation="MHFRNVVCLLSCSLFLSPAWGCKLSSVDPNEYQQQGKGVVYLRP
                     NQNTNLSLPNVNYKRLRRLANLLIDPATLEDWEEIPPLTDLSTDYDVRGANAWYPHYS
                     WHSDGRYIIYAGKIVQNPAGTPPVDVTSFKAWGDFAADKNSLYFRGKRTDDNGGDKPL
                     DSKTLHQRNLPPPWEPDVLGLILRDANFLYINGHRIAETNSFRVLAQKSWDKPGKFSM
                     KFSCIRKPFGPWDTLARTQTKIMLNGEQLDADPDTFTVVRWIPEALISWRDKNGFHRK
                     VLDQYALDSNEEYSCGSFNVKEHRVTWRSSFPGKGAECQQEEIPGLDPEQFHPISDAV
                     AQYQDKLYVIETTPFDELKLNIVTLDDPKLIINKRFNAGKRHGYLLTRKGDEFGDSGL
                     QVFESAGPLILFDNHIPSEREAHQGSSNPYIQKWLARDDKYVYRFDGMQLWRYHTADP
                     RAVRVVNDQLDGKINGDGEFIPTPRDEAKK"
     gene            10722..11978
                     /locus_tag="ACHJWB_000456"
     CDS             10722..11978
                     /locus_tag="ACHJWB_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4312144.1"
                     /translation="MAIPAVQAEGYEYYSNQGALVYGSNFGGQKKIIAKFTGINSRDL
                     YPVENISGEQERYIIEDEVTTIKEEYHWITDGHVILWRGKIVSNPPGTPTVDVASFKA
                     LGRFAVDKYSLYFDGQRTESNSGASKVDLATLKAIEDNSSTLVDRHNLYLLGRRQASS
                     DDFSVLQSKLWGDIERFRPRNEGATSRDLLIRHRQDVFLNGQRIADVDADTFHIIRWI
                     PNSLLVYRDKHGEHRYAYGNPAGKMATTDDANSFEIGEKTVRWRRQLARDDVWHTQLS
                     WDVIWGAWQDIPNVDPEQFHLITDRIAQYQDRLYIIKLSPVGEDQLNVITLDTPDLVV
                     DHVFNGGKKHIYIIKDRTWVQDVHVIATHGPLTMAEGFAWDDRYVYTWWGQRPSRTES
                     PCPEQTVEQDDGIVIKTEASECHRSP"
     gene            12009..13517
                     /locus_tag="ACHJWB_000457"
     CDS             12009..13517
                     /locus_tag="ACHJWB_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4312145.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADLESFRVLAQK
                     SWDQRGKFSTTFNPCIAVPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            13514..14827
                     /locus_tag="ACHJWB_000458"
     CDS             13514..14827
                     /locus_tag="ACHJWB_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4312146.1"
                     /translation="MKGQIITVALILLEHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGPPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIPLDTP
                     DLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            14875..15195
                     /gene="cyaY"
                     /locus_tag="ACHJWB_000459"
     CDS             14875..15195
                     /gene="cyaY"
                     /locus_tag="ACHJWB_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4312147.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(15235..17781)
                     /gene="cyaA"
                     /locus_tag="ACHJWB_000460"
     CDS             complement(15235..17781)
                     /gene="cyaA"
                     /locus_tag="ACHJWB_000460"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4312148.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            18168..19109
                     /gene="hemC"
                     /locus_tag="ACHJWB_000461"
     CDS             18168..19109
                     /gene="hemC"
                     /locus_tag="ACHJWB_000461"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4312149.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRKLLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPT"
     gene            19106..19846
                     /gene="hemD"
                     /locus_tag="ACHJWB_000462"
     CDS             19106..19846
                     /gene="hemD"
                     /locus_tag="ACHJWB_000462"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4312150.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            19868..21103
                     /gene="hemX"
                     /locus_tag="ACHJWB_000463"
     CDS             19868..21103
                     /gene="hemX"
                     /locus_tag="ACHJWB_000463"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4312151.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            21106..22302
                     /gene="hemY"
                     /locus_tag="ACHJWB_000464"
     CDS             21106..22302
                     /gene="hemY"
                     /locus_tag="ACHJWB_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4312152.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            22981..23118
                     /locus_tag="ACHJWB_000465"
                     /pseudo
     CDS             22981..23118
                     /locus_tag="ACHJWB_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(23226..24461)
                     /gene="aslB"
                     /locus_tag="ACHJWB_000466"
     CDS             complement(23226..24461)
                     /gene="aslB"
                     /locus_tag="ACHJWB_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4312153.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(24608..24684)
                     /locus_tag="ACHJWB_000467"
     tRNA            complement(24608..24684)
                     /locus_tag="ACHJWB_000467"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24648..24650),aa:Pro,seq:tgg)
     gene            complement(24727..24813)
                     /locus_tag="ACHJWB_000468"
     tRNA            complement(24727..24813)
                     /locus_tag="ACHJWB_000468"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24777..24779),aa:Leu,seq:cag)
     gene            complement(24834..24909)
                     /locus_tag="ACHJWB_000469"
     tRNA            complement(24834..24909)
                     /locus_tag="ACHJWB_000469"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24874..24876),aa:His,seq:gtg)
     gene            complement(24968..25044)
                     /locus_tag="ACHJWB_000470"
     tRNA            complement(24968..25044)
                     /locus_tag="ACHJWB_000470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25008..25010),aa:Arg,seq:ccg)
     gene            complement(25147..26532)
                     /gene="yifK"
                     /locus_tag="ACHJWB_000471"
     CDS             complement(25147..26532)
                     /gene="yifK"
                     /locus_tag="ACHJWB_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4312154.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(26723..27463)
                     /gene="wecG"
                     /locus_tag="ACHJWB_000472"
     CDS             complement(26723..27463)
                     /gene="wecG"
                     /locus_tag="ACHJWB_000472"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4312155.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYEDGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(27466..28818)
                     /gene="wzyE"
                     /locus_tag="ACHJWB_000473"
     CDS             complement(27466..28818)
                     /gene="wzyE"
                     /locus_tag="ACHJWB_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4312156.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(28815..29894)
                     /gene="rffT"
                     /locus_tag="ACHJWB_000474"
                     /gene_synonym="wecF"
     CDS             complement(28815..29894)
                     /gene="rffT"
                     /locus_tag="ACHJWB_000474"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4312157.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGILCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(29891..31141)
                     /gene="wzxE"
                     /locus_tag="ACHJWB_000475"
     CDS             complement(29891..31141)
                     /gene="wzxE"
                     /locus_tag="ACHJWB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4312158.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(31143..32273)
                     /gene="rffA"
                     /locus_tag="ACHJWB_000476"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(31143..32273)
                     /gene="rffA"
                     /locus_tag="ACHJWB_000476"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4312159.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(32278..32952)
                     /gene="rffC"
                     /locus_tag="ACHJWB_000477"
                     /gene_synonym="wecD"
     CDS             complement(32278..32952)
                     /gene="rffC"
                     /locus_tag="ACHJWB_000477"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4312160.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(32930..33811)
                     /gene="rfbA"
                     /locus_tag="ACHJWB_000478"
     CDS             complement(32930..33811)
                     /gene="rfbA"
                     /locus_tag="ACHJWB_000478"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4312161.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(33830..34897)
                     /gene="rffG"
                     /locus_tag="ACHJWB_000479"
     CDS             complement(33830..34897)
                     /gene="rffG"
                     /locus_tag="ACHJWB_000479"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4312162.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(34897..36159)
                     /gene="wecC"
                     /locus_tag="ACHJWB_000480"
     CDS             complement(34897..36159)
                     /gene="wecC"
                     /locus_tag="ACHJWB_000480"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4312163.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(36156..37286)
                     /gene="wecB"
                     /locus_tag="ACHJWB_000481"
     CDS             complement(36156..37286)
                     /gene="wecB"
                     /locus_tag="ACHJWB_000481"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4312164.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWGRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(37342..38388)
                     /gene="wzzE"
                     /locus_tag="ACHJWB_000482"
     CDS             complement(37342..38388)
                     /gene="wzzE"
                     /locus_tag="ACHJWB_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4312165.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(38400..39503)
                     /gene="wecA"
                     /locus_tag="ACHJWB_000483"
     CDS             complement(38400..39503)
                     /gene="wecA"
                     /locus_tag="ACHJWB_000483"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4312166.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRSLRRNRGGSPNLTK"
     gene            complement(39743..41002)
                     /gene="rho"
                     /locus_tag="ACHJWB_000484"
     CDS             complement(39743..41002)
                     /gene="rho"
                     /locus_tag="ACHJWB_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4312167.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(41087..41188)
                     /gene="rhoL"
                     /locus_tag="ACHJWB_000485"
     CDS             complement(41087..41188)
                     /gene="rhoL"
                     /locus_tag="ACHJWB_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4312168.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(41329..41658)
                     /gene="trxA"
                     /locus_tag="ACHJWB_000486"
     CDS             complement(41329..41658)
                     /gene="trxA"
                     /locus_tag="ACHJWB_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4312169.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            41789..43054
                     /gene="rhlB"
                     /locus_tag="ACHJWB_000487"
     CDS             41789..43054
                     /gene="rhlB"
                     /locus_tag="ACHJWB_000487"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4312170.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            43188..44672
                     /gene="gppA"
                     /locus_tag="ACHJWB_000488"
     CDS             43188..44672
                     /gene="gppA"
                     /locus_tag="ACHJWB_000488"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4312171.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(44719..46740)
                     /gene="rep"
                     /locus_tag="ACHJWB_000489"
     CDS             complement(44719..46740)
                     /gene="rep"
                     /locus_tag="ACHJWB_000489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4312172.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            46957..47406
                     /locus_tag="ACHJWB_000490"
     CDS             46957..47406
                     /locus_tag="ACHJWB_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4312173.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            47605..47886
                     /gene="ppiC"
                     /locus_tag="ACHJWB_000491"
     CDS             47605..47886
                     /gene="ppiC"
                     /locus_tag="ACHJWB_000491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4312174.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(47973..49448)
                     /gene="ilvC"
                     /locus_tag="ACHJWB_000492"
     CDS             complement(47973..49448)
                     /gene="ilvC"
                     /locus_tag="ACHJWB_000492"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4312175.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            49598..50491
                     /gene="ilvY"
                     /locus_tag="ACHJWB_000493"
     CDS             49598..50491
                     /gene="ilvY"
                     /locus_tag="ACHJWB_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4312176.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(50543..52087)
                     /gene="ilvA"
                     /locus_tag="ACHJWB_000494"
     CDS             complement(50543..52087)
                     /gene="ilvA"
                     /locus_tag="ACHJWB_000494"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4312177.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(52090..53940)
                     /gene="ilvD"
                     /locus_tag="ACHJWB_000495"
     CDS             complement(52090..53940)
                     /gene="ilvD"
                     /locus_tag="ACHJWB_000495"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4312178.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(54005..54934)
                     /gene="ilvE"
                     /locus_tag="ACHJWB_000496"
     CDS             complement(54005..54934)
                     /gene="ilvE"
                     /locus_tag="ACHJWB_000496"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4312179.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(54954..55217)
                     /gene="ilvM"
                     /locus_tag="ACHJWB_000497"
     CDS             complement(54954..55217)
                     /gene="ilvM"
                     /locus_tag="ACHJWB_000497"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4312180.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(55214..56860)
                     /gene="ilvG"
                     /locus_tag="ACHJWB_000498"
     CDS             complement(55214..56860)
                     /gene="ilvG"
                     /locus_tag="ACHJWB_000498"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4312181.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(56863..56913)
                     /gene="ilvX"
                     /locus_tag="ACHJWB_000499"
     CDS             complement(56863..56913)
                     /gene="ilvX"
                     /locus_tag="ACHJWB_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4312182.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(57000..57098)
                     /gene="ilvL"
                     /locus_tag="ACHJWB_000500"
     CDS             complement(57000..57098)
                     /gene="ilvL"
                     /locus_tag="ACHJWB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4312183.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT        14009 a        15359 c        14583 g        13239 t
ORIGIN      
        1 aaaataaaat agagcgagtt ttcaatccgc atgactttaa ataagacaat ttttagaaat
       61 aagccggata aaaaaatagc tttatccggc actcatatta cagccagttc ttgcgcttaa
      121 agtacagata cggtgccagg cccgcgagga tcataaagat aatcgcgcct gggtagccga
      181 aactccactt cagttctggc ataaactcaa agttcatccc atagctggaa gcaacgagcg
      241 tcggcggcag gaataccacg gataccaccg agaaaatttt gatgatgcgg ttctgctcga
      301 tgttgataaa acccattgcc gcctgcatca ggaagttcac cttctggaac agggattcgt
      361 tatgcggcag cagggattcg atatctcgca ggatttcacg cgcctgctcc agttgcccac
      421 ccggtaaacg cgctttacgc accaggaagt tgagcgcgcg ctgggtatcc atcagacaca
      481 ggcgaacttt ccagccgata tcttccagtt ccgccagcgt ggagagcgcc tcgtcgtact
      541 catcgccctg atgcccttcc ataatcaccc ggctcaactg ctccaggtcg ctatagatat
      601 tttcaatttc atctgccaac tgttcgattt tggtttcgaa cagatccagc agcaactcgt
      661 aggcgttacc gtctaccatc gactggctac gggcacgcat acgatacaga cgaaaagcgg
      721 gcagttcacg ctcacgcaga gtaaacagac gaccatcacg gatggtaaat gccacagtgg
      781 agttaccggc gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata tgcaggccgt
      841 catcgtcttc aaagaaacgt gccgatgctt cgatgtcttc cagttccggg cgggttgcca
      901 ggctctggcc aagttcagat tgtacgcgca gtcgctcgtc gtcgtccggt tcgacaagat
      961 caatccatac tgcatttaca aggggttgtg actcttcgac ttccagccgg gtcagtcggt
     1021 tattttccag ttgaaatgcg ctcagcatga ccgggactcc caatgcttaa aatatcggac
     1081 agttcggtgg gcacacagaa acaaattggg tttcagacca ttaaacagcc tgactcagcg
     1141 cgacgggaaa aattgaggtc gctgacaacc gctaaggcta tcagcaaaaa gggatagcct
     1201 taggagttga tcctggatga caggatagtg agccagtatc tactgggtgt gtccaaggcg
     1261 aatgtcctct tagagtgatc gtgcgcgcat gttacgccag caaaattttg ccgtcaacac
     1321 gcaacagaac accacagaac aatagttgaa cttcgcgctt tttggtactc gtcaaggtcg
     1381 ctaacaggat aaaaaaatcc aacatccagg acctataatg gcgcataggc tactcattgg
     1441 taaaggaatg atcaccttga atctcaaacg gatttttctc accctcacct tacttccctt
     1501 gtttgctgtt gccgctgatg attgcgcact ctcagatccg acgctgaccg tacaggcgta
     1561 taccgttaat cctcaaacag agcgggtgaa aatgtactgg caaaaagcca atggcgaagc
     1621 gtggggaacg ttacatgctc tgctggcgga tattaatagt cagggtcagg tgcagatggc
     1681 gatgaacggc ggcatctatg atgaaagcta tgcgccgctc ggtttgtaca tcgaaaacgg
     1741 tcagcagaag gtggcgttaa atctcgcttc aggtgaaggg aatttcttta tccgtcctgg
     1801 cggcgtgttt tatgtcgcgg gagataaagt cggcatcgtt cgtctggatg ccttcaaaac
     1861 cagtaaagag attcagtttg cggtgcagtc agggctaatg ttgatggaaa acggtgtaat
     1921 taatccgcgt attcatccca acgtcgcctc acgtaaaatt cgtaacggtg ttgggattaa
     1981 taaacatggg aatgccgtgt ttttgttgag ccagcaggcg acaaattttt atgattttgc
     2041 ctgttatgcc aaagcgaaac tgaacgttga gcagctgctt tatcttgatg gcacgatttc
     2101 acatatgtat atgaaaggcg gggcaatccc gtggcaacgc tatccttttg tcaccatgat
     2161 ttccgtagag cggaaaggtt aaaacgatag ttttgtgtca gatactgaag atggcgcaga
     2221 tgccggatgc ggcgtaaacg ccctatccgg cctacatgac gttgcaattt attgaatctg
     2281 aatgattttt taggccaaat aaggtgcgca gcaccgcatc cggcaacgtt acaccgactc
     2341 cagccgggcg taagctgcca ccagccattt gattccctgg ccctggaatg ccacctgcaa
     2401 acggctatgt tcaccgctgc cttccatatt gacaatggtg ccttcaccaa acttagcgtg
     2461 gcgtacgcgc tggccgagct tgtagccgct gtcgttctcg accatcggcg tacccatccg
     2521 ctgatggctg accgggcggc ttaccgtggc gcgcaggcgc acctcttcca cacactcttc
     2581 cggcagctcg ccaataaagc gcgacgggcg atggtaaacc tctttgccat acagacggcg
     2641 agtttccgcg taggtcagcg tcagtttctg catcgcgcgg gttacgccaa cgtaggccag
     2701 acggcgttct tcttccagac gcccgccttc atccagcgac atctggcttg ggaacatgcc
     2761 ctcttccata ccgacgataa acacctgcgg gaactccaga cctttcgccg agtgcatggt
     2821 catcagttgt accgcatcct gccaggtatc tgcctgccct tcgcctgctt ccagcgccgc
     2881 atgggatagg aacgcctgca gcggcattaa atcttcgtct tcttcgttgt agctgaactg
     2941 gcgcgttgcc gtcaccagtt cctctaagtt ttcgatacgc gtctgacctt tttcaccctt
     3001 ctcctgctcg tacatggtac gcaggccgga gtctttaatt acccggtcag tctgtacatg
     3061 cagcggcata tcggcagttt cctgcgctaa ggcgtcgatc agttccataa accgctgtaa
     3121 ggcgctggca gcacgtccgg cgagggcttt ttcctgcaac agttcacgac atgcctgcca
     3181 gagtgttaac tggcgatcgc gcgatgtctg acgtaccacg tccagcgtcc ggtcaccaat
     3241 accccgcgtt ggcgtattca ccacacgctc aaaggccgcg tcgtcgttac ggttggcaat
     3301 caggcgcaga tacgagagcg catctttgat ttcctggcgt tcgaagaagc gcatcccgcc
     3361 gtaaatacgg tacggcatac tcgcctgcaa cagtgcttct tccagcacac gcgactgggc
     3421 gttactgcgg tagagaatgg cgcactcggc aagcgccccg ccgttgtcct gccaggtttt
     3481 gatgcggtta accacaaaac gcgcttcatc gagttcgtta aaagcgcaat agagggaaat
     3541 aggctcaccg tccgcgccat cggtccacag ttttttaccc agacgcccgt tattgttttc
     3601 aatcagggcg ttagcggcgc tcagaatatt gctggtagag cggtagtttt gctccagacg
     3661 aatagtttcg gcaccgggga aatcattaag gaaacgctga atattctcca cctgcgcccc
     3721 gcgccagccg tagattgact ggtcgtcatc accgacgatc atcactttgc cggtgtcgcc
     3781 cgccagcagg cggatccacg cgtactgaat gttgttggta tcctggaatt cgtccaccag
     3841 gatattggta aaacgttcgc ggtagtgttg taggatatgc ggcttgttaa gccacagttc
     3901 gtgagcgcgc agcagcagct cggcgaagtc caccaggccc gcgcggtcac acgcttcctg
     3961 atacgcctga tacaccttct gccaggtctg ctccaccgga ttaccgtagc tttgaatatg
     4021 atgcggacgc aggccttcat ctttctggct gttgatgtac cacattgcct ggcgcggcgg
     4081 ccactgcttc tcgtcgaggt tcatggcttt gatcagacgc ttaagcaggc gcagctggtc
     4141 ctcgctatcg agaatctgga aatcctgcgg cagattggcg tccatatggt gcgcacgcag
     4201 cagacggtgc gccagcccgt ggaaggtgcc gacccacatg ccgccctggc tggtgcccat
     4261 cagctgcccg atacgatgac gcatctccgc cgccgctttg ttggtaaacg tcaccgccat
     4321 aatcgagtat ggcgagcagt tttccacgct catcagccag gcgatacgat gcaccagtac
     4381 gcgcgtctta ccactgcccg cgcccgccag caccagaagg ttgctgcgtg gcgcggccac
     4441 cgcttcgcgc tgtttgtcat taaggctgtc gagcaggtaa gaaacgtcca ttggcaccgc
     4501 cgcgtaaaaa taggttgggc ggagaagcgc aacgccctcc gccaaaaagc tgggtatata
     4561 tacagatttg ctgattatat cagcgaggtc agagatgcca accgggaaat ttccagatgc
     4621 ggcagtaaac ggctgtccca ggtttgcatc agatcgccat tttccggtct gatccaacag
     4681 gcctgcattc cgctgcgaat tgccccaccc acgtcagtgg tgagatcgtc cccaacatgt
     4741 aagatctcgc cgatcggcac gttgagtttt tccgcagcca aaaagtacat atcgctgaac
     4801 ggttttgagc gcccgtgcgg gccagcgcgc agcacaaact caaaataatc ccccaaacca
     4861 aacagttccg gctgggcgtt accgttggtg atcgccacca gcggccattt cttcgccagc
     4921 tgttttaagg tgtcgtgagt ttgctgcggg acgtcgattc ggctgcgcca tttggcaaag
     4981 ttgatcattg ctgcgtgtgc gcctgcactg gcttcttcgg cactcagccc ggcgtcgagc
     5041 atcgcttgtt caatcgaacg aaaacgccaa cgcgtcacgt cgtgataaat ctcgggttcc
     5101 gcttcccgta ccgcctggcg caggcgttgc agatcttcat tctggaagct gcgcagcgcc
     5161 ggatgataat tttgcacaaa ggtaagcgcc tctcgctcgg tgcgcaaaat caccggacgg
     5221 ttatcgtaaa gggtatcatc caggtcaaag gtgagcgccg agatgcgccc caaaggccgg
     5281 taaaaacgca ttatttcccc cgtttggcgc gtggatgcgc cgcatcgtac accgaggcaa
     5341 ggtgttgaaa atcaagatga gtatagattt gcgtggtgga gaggttggca tgacccagta
     5401 gctcctgcac accacgaaga tcgccgctcg actccagcat atgcgtagcg aacgagtgac
     5461 gtaatttatg tgggtgaacg tgattattca gcccttgttt tatgccccat tcggcaaagc
     5521 gtttctgcac attacgcgcg gagatacgct tgcccagttt cgacagaaaa agcgcgtcgt
     5581 cttcgctacc aaacaggtcg cgcaaatcaa gccagtgctc aatccacgcc acagcgttgc
     5641 gaccaatcgg caggcggcgc tctttgctgc ctttccccat aacccacact tcaccagact
     5701 ccaggtcgag gtgtttaata tccagcccca ccagctcaga aagacgcaga cccgcgccgt
     5761 acatcacttc cagcattgca cggtcgcgta cagcgagggg atcattgata tcaatatcca
     5821 gcagccgatt catatcgtcg acgtcgatgt ttttcggcag atgacgcggc gctttcggtg
     5881 ccgaaacacc tttcgccggg ttagctttga gttcgttctg gctgaccagc cagtcaaaaa
     5941 agctacgtag cgcagaaagc cgtaacgcca gacttgctgc tcccagccct ttacggcgac
     6001 tgcgtacagc aaaattgcgc accatcgtca catcacattg ctgccagctt tgcaggccgt
     6061 tttcgctggc aaaattgatg atcgcctcaa gctgacgctg gtagttaagc agggttatcg
     6121 ggctaagctg gcgctccacg ctcagataac gtaggtagcg ttctacatcg gtgtgtaaat
     6181 cggtcatacg cgttcaatcc aacgctccag aagctccggc aacatcagcg caatttcatg
     6241 aagtaactgc gttccttgcc cttgttgata gtgactggca tcgcgactgg taaacagcac
     6301 gacacccaaa tcagcatcgc ttcccagcat cgacatcgcc accgatccca ccgctttcgc
     6361 ttccggtagc accaccagca gctctggtcc gttaagcggc ccaagatagt gctgttcctg
     6421 ccccaaacgc tgaatacgca gcggttcgaa agactgacgg cttaatgcca gatgagtgtg
     6481 gttcgacggc gcacctaagc gccagcgatc cggaaacagg cgcagactcg cacctgccag
     6541 accgagatcg cgcgcccagc ggtgaaagcg catcagcata tcgtcgagac tgctggcggc
     6601 ggtgagactg cgctgcaggt agagtaagcg ataaaacagg ccttcgttgg cgatagcctg
     6661 ttccatcaac aacgccatgt tctcttccag aacatgaata tgattacgtg cgcgggccat
     6721 gtgccattcg accaacgaaa cggtgccgcg taccggatgc ggcacacgta tcgcttctac
     6781 tgcgcgcgca ttacggataa aaaactcagg atttttaatc agataatcga caaccgcccg
     6841 gtcatcaagc tccgtgagtg tttcctgcag ttcttcccct ggttgcttca tagatgaata
     6901 aatccgtcgt agacatgtac cgccgggcca gtcatatata acgggtgacc cggacctttc
     6961 caggcgatat caagacgacc gccggggagt tccacgcgta cttcttcggc cagcaaacct
     7021 tgctggatcc ctaccgccac cgccgcacac gcgccgctgc cgcaggcctg ggtttctcct
     7081 gccccacgct cataaacgcg taaacgaata tgctcgcgct taaccacttg cataaaaccg
     7141 atattggctc gctccggaaa acgctcgtgg ctttccagaa caggaccaag cgtttctacc
     7201 gccgcggtat cgacatcatc gacctgaatc acgcaatgcg gatttcccat cgacaccacg
     7261 ccgcataaga ttgtctgctc ggcggcgcgc ataatatagg tcttttccgc tttgttagcg
     7321 cgaaacggca cggcggaagg ttcgaagttg ggttcgccca tatttacgcg gaccagatca
     7381 tcatcggtga cggtcagaac catccgcccg ttggcggtgc tgacgcggat atcgcgctta
     7441 ttggtcagcc ctttcagacg cacaaaacgg gcaaagcagc gcgcaccatt gccgcactgc
     7501 gccacttcac tgccatcagc attgaaaatg cgatagtgaa aatccagttc aggatcatac
     7561 ggcggctcaa ccaccagcag ttggtcaaac cctactccca ggtgccgatc agccaggcga
     7621 cgaatcagct ccggtgaaaa aaagacattc tgcgttaccg cgtcgacgac cataaaatcg
     7681 ttgccaaggc catgcatttt cgagaactgc atcatttact ccaatcacgc gggtacagaa
     7741 actgactttt agtaattcac ctgggatgga ccatcgccag tggcgcgatc gtttttatcc
     7801 ggcaccgtgg attgcgtttg cgtctctacc ggtttggtcg gcggcggtgc gtttttatct
     7861 gcagacggga aatagagcgg acctttcaga ccgcagcccg tcaggctgaa gagagtaatt
     7921 aatacagtga gtgccttaaa cacgtttttc attatggatt gcctgtaaag ttcatcgctt
     7981 tctgctttct atcatcgcag gagaaggcgt aaaagcaagc gtttagcgac tctttatgaa
     8041 gtctgaaaag tgaggaaatg ctgcgagtat aacgcttaca cgtggggatt tatgttttgc
     8101 tggtagtttc gtgatcggca cctcgcgcca ggatggcttt atgcactatc gcaatgtggt
     8161 ttgtttgttg tcttgttcat tgttcttatc gtcggcatgg ggcttttaat agagtggcgc
     8221 accatgcaat ttcgttgtct gaccttagtc gccagtgaca tacaactttg ccaacctggt
     8281 gataagaggg aataaaatct gatggggcta tttacccaac tggaaaaatt tgaccaaaag
     8341 cttacgcgcg gttatgcccg ctgggggcgc tgggtatggc ggacgttaat tgtcgttccg
     8401 gtactggtcg tgttgtggaa tataggacag gcagtatggg gaggaccaag aggtggagtg
     8461 attctggaaa tccacagtga aattgaccgt ccgatactgg gattcagtgt taacggcgtg
     8521 gcgggcgcga atgcttttgc aaatggtggc ggttcaatca cctgttgtgg tgatgtcagt
     8581 ggggatacgg cagaggttat ctggacatta gatatgactc acgagcaata tcttaaaggg
     8641 atgcgtctgg agaaacgtaa caagactttg cctctcccaa aaagagaatg gggagaaaat
     8701 tatctgcatg tgcattttat gcctggcgat aaagtgctgc tggggtggag taaagattct
     8761 ttttctccgt atgaagatct tcacaacggg gggtacaaga cccgcgttcg gcaggatgtg
     8821 aaagataaac tttatggtac agggaaaatg aattgataga tttctataaa aatcacgttc
     8881 ggcagaaagc gtaataatgt ggcgaatgtt agatacccgt ggtggaacct gggtacaact
     8941 cgtgaaagac aataaagaag gcaacggggg gcggaatccc accttgcctc ccttccaggg
     9001 gtcaatccgg aatattttta tgctattagc cgtactatgg ggcagttccg ggattgtatt
     9061 tatatcgtga atgaaaatat cacatttatt ccagcaacta tcagaaaata gcgatgacct
     9121 ttatggattc tgaaaagtga cgatttgctg cgagagttac gcttacttgt gaggatttat
     9181 gttctgctgg tagtttcgtg atcggcacct cacgccagga tggctttatg cactttcgca
     9241 atgtggtttg tttgttgtct tgttcgttgt tcttatcgcc tgcctgggga tgtaagcttt
     9301 catctgtgga tcccaatgag tatcagcaac agggaaaagg cgtggtgtac ctgcgcccta
     9361 atcaaaacac caatctttcg ctaccgaacg tgaattacaa gcgcctgcgt cggttggcga
     9421 atttgttaat tgacccagcg acactggaag attgggagga aataccgccg ttaaccgatc
     9481 tgtctactga ttatgatgtg aggggcgcaa atgcctggta tcctcattac tcctggcata
     9541 gcgatggtcg ctatattatt tacgctggca agatagtgca aaatcctgcg ggtacaccgc
     9601 cggttgacgt cacgtcattc aaggcttggg gcgattttgc cgctgataaa aatagcctct
     9661 atttcagagg caaacgcacg gatgacaatg gtggtgacaa gcctttagat agcaaaacac
     9721 tgcatcagag gaatttaccg ccgccatggg aacctgacgt cctcgggctg atattgcgtg
     9781 atgctaactt cctgtatatc aacgggcatc gtattgctga gaccaatagt ttccgcgtgc
     9841 tggcgcaaaa gtcctgggat aagcccggaa aattttccat gaagtttagc tgcattcgca
     9901 aaccattcgg cccctgggat acgctggctc gtacacagac gaagattatg ctcaacggcg
     9961 aacaactgga tgccgacccg gataccttca ccgttgtgcg ctggatacct gaagcattaa
    10021 taagctggcg agataaaaac ggctttcacc gaaaggtact ggatcaatat gccctggaca
    10081 gcaatgagga atatagctgc ggtagtttca atgtaaagga acaccgcgtc acctggcgta
    10141 gtagcttccc gggtaaggga gcagaatgcc aacaagaaga gattcccggc ctcgatcctg
    10201 aacagtttca cccaattagc gatgctgtcg cgcagtatca ggacaagctg tacgtcatcg
    10261 aaacaacgcc attcgacgag ctcaagctga atattgttac gcttgatgac ccgaaactga
    10321 ttattaataa acgcttcaac gccgggaagc gtcacggtta cttgctcaca agaaagggag
    10381 acgagtttgg ggattctggt ttacaggtgt ttgaatccgc cgggccactc attttatttg
    10441 ataaccacat ccccagcgaa cgggaagcgc atcagggtag cagcaaccct tatatccaaa
    10501 aatggttagc ccgcgacgac aaatatgttt atcgcttcga tggcatgcag ctctggcgct
    10561 atcacaccgc agatccccga gcggtgcgcg tcgtgaacga tcaactcgat ggcaaaatta
    10621 acggtgatgg cgagtttata cccactccgc gcgatgaggc taaaaaatga aagtgataaa
    10681 aaaaaagtat ctcactggcg ctcatcctgt tggggtacac ggtggcaatt ccggccgtcc
    10741 aggctgaagg gtacgaatac tactctaacc agggtgccct ggtttacggt tcaaacttcg
    10801 ggggacaaaa gaaaatcatt gccaagttta cgggaattaa cagccgcgat ttgtaccctg
    10861 tggaaaatat ctctggcgag caggaacgat atattatcga agatgaagtc accacaatta
    10921 aagaggagta ccactggata acggacggtc acgtcattct ctggcgaggg aaaatcgtga
    10981 gtaatcctcc cggtacgcca acggtcgatg ttgccagctt taaggctctg ggccgcttcg
    11041 ccgtcgataa atacagtctc tattttgacg gtcagcgcac cgaaagcaat agcggcgctt
    11101 cgaaggtgga cctggcaacc ctaaaagcta tcgaagataa cagcagcacg ctggttgacc
    11161 gacataacct ttatttattg ggacgcaggc aggccagtag cgacgatttc agcgtactac
    11221 aaagtaaatt gtggggggat atcgagcggt tccgcccacg caatgaaggg gctacctccc
    11281 gcgacctgct tattcgtcat cgccaggatg tgtttctcaa tggtcagcgc attgcagacg
    11341 ttgatgctga cacctttcac atcattcgat ggatccccaa ttcattattg gtctaccgcg
    11401 acaaacatgg tgagcaccgt tacgcctatg gcaacccggc aggaaaaatg gcaacgaccg
    11461 atgatgcgaa ttcgtttgaa atcggtgaaa aaaccgtccg ctggcgcagg caactcgcgc
    11521 gggatgacgt ctggcacacg caactctcgt gggatgtcat atggggggcc tggcaggata
    11581 tccccaatgt tgatccggaa cagttccatt tgatcactga ccgcatcgcg cagtatcagg
    11641 atcgtctgta tatcatcaaa ctgtcgccag ttggcgaaga ccagcttaac gttatcaccc
    11701 tggatacccc tgatttagtg gttgatcacg tcttcaatgg tgggaaaaag cacatttata
    11761 ttatcaagga cagaacgtgg gtgcaggatg ttcatgtgat tgctacccac ggtccactca
    11821 ccatggctga gggtttcgcc tgggacgatc gctatgtata tacctggtgg ggccagcgtc
    11881 cttccagaac tgagtcgcca tgtccggagc aaaccgttga gcaggatgat ggcatagtca
    11941 ttaagaccga agcatcagag tgccaccgct cgccgtaaaa tgggtcatta tttttaacgg
    12001 gtagtttcat gatcaacacc tcgcgccagg atggctttat gcactatcgc aatgtggttt
    12061 gtttgttggc ttgttcattg tttttatcgt cggcatgggg atgtcggctt tccgcacctg
    12121 aacataatat ttaccagaaa cagggaaaag gcgtggtgta cctgcgccct tataaagaaa
    12181 caaatctttc tctacctgac gtgaattaca agagtttgcg tcggttaccc aatttattaa
    12241 tagacccgac aacactagac gaatgggata aagaaccgcc tctgactgat ttgacaaccg
    12301 actacctcta tgagggcgca caggcctggt atcctcatta ctcctggcat agcgatggtc
    12361 gctatatcct ctacgcgggt gaagttgtgc aaaacccgcc agacaaacct cccgtcgatg
    12421 tcgcctcgtt caaggcgtgg ggtgattttg ccgcagataa acacagtctc tatttcgaag
    12481 gcaagcgcac cgataacaat ggcgggggaa atcgcttaga tatcaaaaca ctgcatcagg
    12541 tggaattccg gcctccctgg gatcctgact tactggggct gatattacgt gacgctaatt
    12601 tcttgtatat caatgggcat cgcctcgctg acctcgagag tttccgtgtg ctggcgcaga
    12661 agtcctggga tcagcgagga aagttttcca ccacgtttaa cccctgcatt gccgtaccat
    12721 ttggcccctg ggataccctg gctcgtacac ggaccaaaat cctgatcaac ggcgaacagc
    12781 ttgatgccga cccggacacc ttttccgtcg tgcgctggat gcccggctca ctcttgacct
    12841 ggcgtgataa aaacgggcta cagcgtaaag tcctcgacaa ggaaaatctg gcgtgggatg
    12901 aagatttaac aaagcactgt ctggattttt ctctgctgga aaagaaagtg ttctggcgta
    12961 aaggacctgc ttgtaaacag gaagaattac ccggactcga tccggagcag tttcaaccca
    13021 tcagtgatgc tgtcgcccag tatcaggact cgctttatac catcatcgaa acagagtctg
    13081 gtgaccgcaa gctggaaatc gtgaaacttg atgatcccaa tcttattatc aacaaacgtt
    13141 tcaacgccgg gaaacgccac ggctatttac ttacgcgtgc cgaagggtgg ccataccatt
    13201 ccggtttaca tgtgtttgaa tctgacggac cgctgatctt actggataac cgttctccgg
    13261 atgaacgcga agcccatctt aatgaccatc cctttttgcg cagatggtat gcccgcgata
    13321 accgctacat ttacagcttt gatggcgcgc agctctggcg ataccgcacc gctgatccga
    13381 aacaagttcg cttaatctgg aaggaacaac attcgggata tggctatggc gtaaattaca
    13441 aaacgggata tctggacgga aaaattactg atgacggcga atttattcct gccccgcgca
    13501 atgaggcgac aaaatgaagg gtcaaataat cacagttgca ctcatcctgt tggaacacac
    13561 atttctgacg cctgctgtac aggccatagg gttcgactat tacaatgatc acggtgttat
    13621 gtcttacggt aaaggttatt ggggggataa gaaaatcatt gcccagtttc cggagatgag
    13681 cagggccgat ttgcgccttg tgaaaaatat ctctgggaaa acgggttata taccttccga
    13741 tgaaataagc ataaaaaatg aggaatacca ctgggtgaca gacggccgcg ttattctctg
    13801 gcgtggcaaa atcgttagca atccaccggg accccccact gtcgatattg ccagctttca
    13861 ggctatgggc cgcttcgcgg tcgataaata tagcctctat tttgacggac agcgcaccga
    13921 aagtaatagc ggcgcgtcac gtgttgatct ggcgacacta aaagctatcg aaggtaactc
    13981 taccacgctg gtggatagta aaaatctcta tttgtccggt cgacgtcagg ggagcagcag
    14041 taatgttact gtattagaaa aaagatggtg gggtattaat ccacgtctta tgagtgtaaa
    14101 cagaaatttg tattccaatg atctgcttat ccgcagtggg cagaatattt atttaaatgg
    14161 cgttcacctt acggcgaatg cagactcatt tgagataatt cgctggatac ctcattcact
    14221 gctggttttt cgcgacaata agggtatgca tcgttatccc tttggtcaat tatcaggcaa
    14281 agcgataccc gtagatgatg acgtctcttt tgaagtaggg gaaagtcgcg ttcgctggcg
    14341 taaacagctc acgtccgacc gtcagtggag caagtggata gacctaccag gtattgaacc
    14401 tgaacaattt catctgatta ctggcaatat tgcgcaatat aaagatcggc tgtatgtaac
    14461 aaaattatcg atatttggtg aagaccagct tgagataatc ccgctggata cgccagacct
    14521 ggtcattgat cgctcattta atggcggcaa acaacatgct tactttatcc gccaattacg
    14581 gtcaaagagc ttgcaaatta ttccagttaa cggtccgcta actaaaaacg atcgcttcgc
    14641 ttatgacgat cgcaatgttt atacatggac cgatacagag gtaaggatta cgccctcccc
    14701 ctgcccggcg aaaactcgtg tcagagagga gaacgtacgt gaagttcaaa acagagacat
    14761 cattattccg gtgacggatg aatcatgccg gaacgcagca gcagaggtgc aaactttgaa
    14821 gccctgaccc catgccgcta tactgccgcc atcacttaaa aacaggatac aacaatgaac
    14881 gacagtgaat ttcatcgcct ggctgatcaa ctgtggctga ccattgaaga gcgtctggac
    14941 gactgggatg gcgacagcga tatcgactgc gaaatcaacg gcggcgtgct gaccattacc
    15001 tttgaaaacg gcagcaaaat cattatcaac cgccaagaac cgctgcacca ggtatggctg
    15061 gcaaccaaac aaggcggcta ccattttgat ctgaaaggcg atgagtggat ttgcgatcgc
    15121 agcggcgaaa ccttctggga tttgctggaa caggcggcga cgcagcaggc gggtgaaaca
    15181 gtcagtttcc gctaagattg catgccggat aagcctcgct ttccggcacg ttcatcacga
    15241 aaaatattgc tgtaatagcg gcgtatcgtg atcctgattg gcaggcggca tgttaccgat
    15301 agattttgtg cggaacggaa tcacctgttc acgaccatca accttcacaa tctgatagaa
    15361 ctgcggcagg ttgaagttga tgaagcttga gccgtaggtg aagcggtcgt gcgatgacga
    15421 gtagaagcga ctgacgtcac gcaccagttc ctctttgctg ccttcgcagt ggtgatacac
    15481 ctcaacccgg ttgctttcgt caagaatgta gatattaaag ccattctcgt cttgcgtttc
    15541 ttcgaagaag aactggatga tcccttcact agcaaagccg tccaccaccg ccggtaattt
    15601 gacgtgattg gtttcaacct gcactgacag gccgtgcagt ttgttatgcg aaatagcgcc
    15661 ataaaactcg atggcgtttt ccagtttttg taccgataca ttcaggcgtt cgaagaacaa
    15721 cccccaggtt tgaccagaaa cgcgcagcgc cttgaaacgc ccggtttcct ggcgggtgct
    15781 ggaaaggcgc agttcaatac actcagaaac cagttgctgc acgcgagtac ggattaaccc
    15841 gcgcagatgc tggctataac agaagacttc cacgctatct ggcggtgcgg cgtcctgatg
    15901 cattttgccg agaatagttt tcagggcttc gatcatcgat tgctcgccgt tgaagtgcag
    15961 cgtacgcact tcgttccacg agttgcggta cagcaggtca acgctaccta ccaggcaatt
    16021 ttgattctcg ccaaagctga agacatccag cttacggaaa tcaaaatgca ccacctgatt
    16081 gcggaacgcc gctgtcgggt catattccag gttaacgata atcgccagat ggcggatctc
    16141 acacgggctg tagagcgcct tcggtgtcgg tgcaggtaag cgcagcggga aatggtgcga
    16201 cacgtcggcg accatctcct gcaacttagg caaatcgaca atgccgttac ctttaatata
    16261 caaacgggtg cgcgaggtca gcaggccgtt aaaccatgcc cacgccacca gtttgttcag
    16321 gtaacggtta tattccagcg gctgatggct gatgatcgac tcaatatttg gcgcgcggtt
    16381 atacagatac caacctgaac ggttagcacg gcccggcggc acataaataa aggtcagatt
    16441 cggttccgag agatcgggtg aaatctgcgg gtttaccagc gtcactttac ctggtaatgc
    16501 ttcaaacgcg gcatacagct tacgcgtcag cacgccgata tcctgcggac tggcggagac
    16561 gctaaggtta ttgcgacgcg caaagcggat cagattacgg tagctctgca tcatcgcgtc
    16621 gagcaactcg ttgtgcgcct cacgcacctg atcaatcttc cagttagcgc ggttatcgag
    16681 cattgccaga cgagcttcgt cccaacccca ctcgctcact aactggctca acactgcgcg
    16741 acgccagcct acgcaggcgc gttcacggct gagcttttcg cacactttta aatagaagca
    16801 gcgacgtact aaatccagac gggtaaaatc ttcaatcgcc gtcaggtatt cagtaacacg
    16861 ctccagcatc atgcagtatg gatcgagacc aaacgataca atctcgccgt cgtgcaaacg
    16921 ctgtttgata tctttcgcca gcagacgtgg gttcgggtat tcccaggaat aggcttccag
    16981 cagcagtgtt ttcagtaccg ctttgtatgg ggaatcgata ctcttgtaga gctgccaaag
    17041 gctggcacca aagtactctt cagcagaaag cgagcttaag ccaccgagat ccagccattc
    17101 atttggcgtc agcacgccct gcgcgtaaag cgtcatcaca tagtcgtcgt aatgctcttc
    17161 ttcgtcgcac ggcaccatat tccacagaat acgcttaccg gcgagacgca cggcggtacg
    17221 ataaaattcg tcaagcagca gtatatgctg ggtggagcca caatcttcgc cccccaggct
    17281 gccgctttca ttatgacgga agcggttttc atcaatcagg aagaagctga cttccacacc
    17341 cagcgaggcg gcccagcttt ccagcagact acatttacgt tgtagtaact ggcgctcttc
    17401 gctatcgagc caggattgat gacagaccca gatatccagg tcagaggaac aactttgccc
    17461 tacggacgag gtgctgccca tggtgtatac accagtaatt ggaagctcac ctttcggtgg
    17521 gtcctgtact gacattccac gatacagttc aagctcgttc aggtagtggc gttgagtttc
    17581 atcaggcgtg taaaggcaaa tgcctttggg aacgttacca tcaaggtaac ccggcattag
    17641 cggatggtga tagtgcaaca atgtcggcag tagactgtag acctgttgga atgcaggccc
    17701 catagcagca agcgcgcgat ccacacgcaa ttgatttatg gcatccagtc tctgtttcag
    17761 agtctcaata tagaggtaca agacgtatcg cctgatttgc tacccgtcat gactgtgatt
    17821 ccgccaacat caacggtaac acgcggcatt cgggatattt cgtatgtcaa aggtaaccgt
    17881 taccactttt cgcgcctggt ttttttagtt tcacgacgaa aaaatggtct aaaacgtgat
    17941 caatttaaca ccttgctgat tgaccgtaaa gaaagatgcg ctacatacaa gtgtagcacc
    18001 gtttattctc tgtaaattcc ttattacgac ggcgtgaaac gcctgtcagg atccactgcc
    18061 agacctcatt ttacggtttg cgcaggcgtc tacgtttcac cacaacactg acatcactct
    18121 ggcaaggatg ttaggatgga ccacggatga taatgacggt aacaagcatg ttagacaatg
    18181 ttttaagaat tgccacacgc caaagcccac ttgcactctg gcaggcacac tatgtcaaag
    18241 acaagttgat ggcgagccat ccgggcctgg tggttgaact ggtacctatg gtgacgcgcg
    18301 gcgatgtgat tcttgatacg ccgctggcga aagtaggcgg aaaaggctta tttgtaaaag
    18361 agctggaagt cgcgctcctg gagaatcgcg ccgatatcgc cgtacattca atgaaagatg
    18421 tgccggttga attcccgcaa ggtctggggt tggtcactat ttgtgagcgt gaagatcctc
    18481 gcgatgcctt tgtgtccaat aattatgaca gtctggatgc gttaccggca ggcagtatcg
    18541 tcgggacgtc cagtttacgt cgccagtgcc aactggctga acgccgcccg gatctgatta
    18601 tccgctccct gcgcggcaac gtcggcactc gcctgagcaa actagataac ggcgaatacg
    18661 atgccatcat tcttgccgta gccggactaa aacgtttagg tctggagtca cgtattcgcg
    18721 ccgcgttgcc acccgagatt tctcttccgg cggtagggca aggtgcggtg ggtattgaat
    18781 gccgccttga tgatgcacgc actcgcaagc tgcttgccgc gctgaatcac cacgaaactg
    18841 cactgcgcgt taccgcagaa cgcgccatga atacccgtct cgaaggcgga tgtcaggtgc
    18901 caattggtag ctacgccgag cttattgatg gtgaaatctg gctgcgtgcg ctggtcggcg
    18961 cgccggacgg ttcgcagatt attcgcggtg aacgccgcgg tgcgccgcaa gatgccgaac
    19021 aaatggggat ttcgctggca gaagagctac tgaataacgg cgcgcgcgag atcctcgctg
    19081 aagtctataa cggagacgcc ccgacatgag tatcctggtc acccgcccgt ctcccgctgg
    19141 agaagagtta gtgagccgtc tgcgcacact ggggcaggtg gcctggcatt ttccgctgat
    19201 tgagttttct ccgggtcgac aattaccgca acttgctgat caactggcgg cgctggggga
    19261 gagcgatctg ttgtttgccc tctcgcaaca cgcggttgct tttgcccaat cacagctgca
    19321 tcagcaagat cgtaaatggc cccgactacc tgattatttc gccattggac gcaccaccgc
    19381 actggcgcta cataccgtaa gcggacagaa gattctctac ccgcaggatc gggaaatcag
    19441 cgaagtcttg ctacaattac ctgaattaca aaatattgcg ggcaaacgtg cgctgatatt
    19501 acgtggcaat ggcggtcgtg agctaattgg ggataccctg acagcgcgcg gtgctgaggt
    19561 cactttttgt gaatgttatc aacgatgcgc aatccattac gatggtgcag aagaagcgat
    19621 gcgctggcaa tcccgcgagg tgacgacggt cgttgttacc agcggtgaaa tgttgcagca
    19681 actctggtcg ctgatcccac aatggtatcg tgagcactgg ttactacgct gtcgactatt
    19741 ggtcgtcagt gagcgtttgg cgaaactcgc ccgggaactg ggctggcaag acattaaggt
    19801 cgccgataac gctgacaacg atgcgctttt acgggcatta caataactct cataacagga
    19861 agccataatg acggaacaag aaaaaacctc cgccgtggtt gaagagacca gggaggccgt
    19921 ggacaccacg tcacaacctg tcgcaacaga aaaaaagagt aagaacaata ccgcattgat
    19981 tctcagcgcg gtggctatcg ctattgctct ggctgcgggc gtcggtttgt atggctgggg
    20041 taaacaacag gccgtcaatc agaccgccac cagcgatgcc ctggctaacc aactgacggc
    20101 attgcaaaaa gcccaggaga gccaaaaagc cgagctggaa ggcattatta agcaacaagc
    20161 tgcacagctt aagcaggcga atcgtcagca agaaacgctg gcaaaacagt tggatgaagt
    20221 ccaacaaaag gtcgccacca tttccggcag cgatgctaaa acctggctgc tggctcaggc
    20281 cgattttctg gtgaaactcg ccggacggaa gctgtggagc gatcaggacg tcacgaccgc
    20341 tgcagcgttg ctgaaaagtg cagatgccag cctggcggat atgaatgacc cgagtctgat
    20401 taccgttcgt cgggcaatta ccgatgatat cgccagcctt tctgcagttt cgcaggtgga
    20461 ttatgacggc atcatcctta agcttaatca gctttcaaat caggtagata acctgcgtct
    20521 ggccgataat gacagcgatg gttcgccgat ggattccgac ggtgaagagc tttccagttc
    20581 catcagcgaa tggcgtatca atctgcaaaa aagctggcag aactttatgg acaacttcat
    20641 tacgattcgc cgtcgtgatg acaccgccgt accgctgtta gcgccaaatc aggatatcta
    20701 tctgcgcgaa aatattcgct ctcgcctgct ggtcgcagca caagctgtac cgcgtcacca
    20761 ggaagagact tatcgccagg cgctggagaa cgtctccacc tgggtacgtg cttactacga
    20821 tactgatgat gccaccacca aagcgttcct cgacgaggtg gaccagttaa gccagcaaaa
    20881 tatctcgatg gatcttccgg aaaccctgca aagccaggcg atgctggaaa aactgatgca
    20941 gacccgcgtg cgtaacctgc tggcacaacc ggcagcgggg acaacggaag ctaaacctgc
    21001 acctgcacct gcacctgcac ctgcacctgc acctgcacct gcacctgcac ctgcacctgc
    21061 accgcaaggt gatactccgg cagccgcgcc gcaaggagaa taaccatgct aaaagtgtta
    21121 ttactctttg ttttgctgat tgcggggata gtggttggcc cgatgattgc cggtcatcag
    21181 ggttacgtgc tgatccagac tgacaactac aatatcgaaa ccagcgtcac gggcctggcg
    21241 attattttga ttctggcgat ggtagtgctg tttgccattg agtggctact gcggcggatc
    21301 ttccgcacag gcgcacacac ccgtgggtgg tttgtcggac gtaagcgtcg ccgtgcccgt
    21361 aagcagaccg aacaggcgct gctgaaactg gcggaaggcg attatcagca agttgaaaag
    21421 ctgatggcga aaaatgccga tcacgcggaa caaccggtgg tgaactatct actggctgcc
    21481 gaagccgcgc aacaacgtgg tgatgaagca cgcgccaacc aacatctgga acgcgcagca
    21541 gagctggccg gcaatgacac cattccggta gaaatcaccc gtgtacgtct gcaactggcc
    21601 cgtaatgaaa accatgctgc acgccacggc gtggataagc tgctggaagt tacgccacgc
    21661 catccggaag tattacgtct ggcggaacag gcgtatatcc gcacaggtgc atggagttcg
    21721 ctgctggata tcatcccatc aatggcgaaa gcccatgttg gtgatgaaga acatcgtgca
    21781 atgctggaac aacaggcatg gattggcctg atggatcagg cgcgtgccga taacggtagt
    21841 gaaggtttgc gtaactggtg gaaaaaccaa agccggaaaa cgcgtcatca ggtagcgttg
    21901 caggtggcaa tggcggaaca tcttattgaa tgtgacgatc atgatactgc ccagcaaatt
    21961 atcatcgatg gcctgaaacg ccagtacgac gatcgcctac tgctgccgat tcctcgactg
    22021 aaaaccaaca atccggaaca gctggaaaaa gtgctgcgcc agcagatcaa aaacgtcggc
    22081 gatcgcccgc tgttgtggag cacactgggc cagtcactga tgaagcacgg agaatggcag
    22141 gaagcatcgc tcgccttccg cgcggcgctg aaacaacgtc cggacgccta cgattacgca
    22201 tggcttgccg acgcgctgga cagactgcac aagccggaag aagctgcagc gatgcgtcgc
    22261 gacggtttga tgttaacgtt gcagaacaac ccaccacagt agttccttct cacccggagg
    22321 caagcacctc cgggaccttt ctgatgcata aaaaaacgcc tgctcttatt acggagcagg
    22381 cgttaaaaca ggtctgtatg acaacaagtg ggtgcttcac tcaacgttgt gtccatggtg
    22441 tctgatgagg cataagcgac atctgtcagt ggacgataag caccgtaaac ggctctgcgt
    22501 cattccggag tttatgaggc actaaggcga acataagaga tggaatgagc atctacccgt
    22561 ttattatgcc acagagaatc gggaaataac atcccttaac acttgttatg agataattct
    22621 gtaatcctct ttgcttcctg agtaataact tcctgagtga atatttaacc tgagcttgat
    22681 cctacacata cttattatga atgataaaat tcattcaatt aataacacat atattaattg
    22741 ccgttaaaac taaaaaaagc accaataatc aacgcgatat aataaacctg ccttacatat
    22801 caactgcgcc agaggtagga ttgaaaacgc tctcctgatt ttccaattca ttttctggat
    22861 aaataaataa tttatttttg tcactattat ttatgtaatc atcctgtcag ggagagggat
    22921 ctcaattatc aatgcttaat tacgtcatca ttttgatgac atgaaaccgg agcattttgc
    22981 atggaatttt cgttttcacc caaacttctt gttgttgctg tcgccgccgc tcttcctctc
    23041 atggccagcg cagcagatac cccgtcaact gccaccgcac gcaaaggctt tgccggatac
    23101 gatcacccaa accagtatcc acagaacgct gcaatttatt gaatttgcac gatcatgtag
    23161 gccggataag gcgtttacgc cgcatccggc aatcaaccgc aggcggccac aaaatggccg
    23221 cttttttact tattcaccac cagcaaatgt gcctgcataa tgtcactggc cgggcgaccg
    23281 tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa ataacgctga
    23341 tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat gaagcggtgt
    23401 ttcgggcagc ctccccagca cgcttttaac acgttacaac tgcgacactg cgccggtaat
    23461 tgcttaaatt tatcttcacc aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt
    23521 gtttgctgat gcatattccc cagccgatat tgcggataaa catagtgatc gcaggcgtaa
    23581 acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg atggcaaacc
    23641 gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac gaaaatcttg
    23701 ccgacgtcgc gtttgatcca gtggtcgaat atcgccacca gaaattcacc aaattcatcg
    23761 gggcgcaccg accattccgt cagttccccc tgaatatcac caggcgcatg tagtttcagt
    23821 ccatcgctgg cagctgtttc atcagccagg cgctcgacca ccggaataaa ctggatgaat
    23881 tcgactcccg catcgcacaa aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg
    23941 ttgacgcaga ccagcacgtt atagtcgaca tgatgttttt gcagcagcgt cagggcacgc
    24001 atcaccagct tatgcgttgg tctgccacct ttggtcacgc gatattgatt gtggatctca
    24061 gccggaccgt ccagcgataa cccaacaaga aaatgatttt ctgccagaaa tgcacaccat
    24121 ttatcatcga gcagcacgcc gttagtctgg aagctgttac ttatcttcct gccagcaccg
    24181 tatttcgcct gtaactttac ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg
    24241 ccgccctgcc aggtaaaagc cacttcgttt tgcggttcgc tggcagcgat atagtgacgg
    24301 acatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg gtaaagggat
    24361 tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc actcggtttc
    24421 gccatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcactcc ttgcctgaag
    24481 tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta ttacgggcgt
    24541 ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt
    24601 tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca aaccagttgc
    24661 gctaccaagc tgcgctactc gccgaaatac tgctttttga atttttagtt caattcttta
    24721 aagtcgtggt gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga
    24781 agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa taatggggtg
    24841 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
    24901 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgccgg
    24961 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
    25021 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
    25081 cacccctctg tctagtccct ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt
    25141 tgctgcttat tcctccagtt tatgcgcttt cccgtggcga ttaaggccaa aaactttata
    25201 aatcgccgtc accgccagca taaagatgat gccaacaaac agcgacatac gcgtatcttc
    25261 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
    25321 cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg cacgccgaaa
    25381 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
    25441 gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
    25501 tagcagaata gcaatagata ctgccacacc cgcaacgggt acgccgtgac gggaaacttt
    25561 cgccatcgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
    25621 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
    25681 cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc
    25741 tatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag
    25801 gatccgccac agcaccttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
    25861 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacagcgc
    25921 ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa
    25981 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaggc caatgacaat
    26041 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
    26101 ccgcaccgcc gccagattcg ccaacgccac cagcgccact gcgatcaatg cgggtatcca
    26161 ctgcgccatc tccgggaacc agaactggac ataaacgcca atggcggtga tttctgagat
    26221 ccccacggcc atccacataa accaataaga ccaggcggtg agataaccaa agaacgggct
    26281 catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga acaacatttc
    26341 gcccattgaa cgcatgatga aaaagacgaa tagcccggcg atgatatagg ccaacaatac
    26401 ggatggcccg gcccatttca aggtactggc ggcccccata aacaggccga cgccaatggt
    26461 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
    26521 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
    26581 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
    26641 ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacggagt gaccactccg
    26701 tcgctttaca aagagaggaa aatcagaggt tgccggtgta gtgccagcgt aaataacgca
    26761 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
    26821 gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag
    26881 tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg cgcatgaaga
    26941 tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc
    27001 gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg ccaacgatat
    27061 tcacattcca ctggttgcgc agtttagctt cagtttgcgc cagcacttca ggtttaccgc
    27121 ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct tcccagagat
    27181 cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact gaacgtacaa
    27241 cgctgatgcc atcctcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt
    27301 tatcttcaat ggtcagcatt ttttcagcat taatggcaac cagcgttccc tgcttaagct
    27361 gcccgtcagc aaacagataa tcgagggcgt gctgcatatc acgccaacca atcaactgta
    27421 agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg
    27481 cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta
    27541 caacagtttt gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga
    27601 gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg ccccaaagca
    27661 gaaactgtgc aatatcgcag ccttatagcg attaggctcg cggttgccca gctcatacag
    27721 ccagtcgaac catttgatga tcagaccaac cacgatcgcc ccgagcggga tgaacaacgc
    27781 gccgcccatc accaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt
    27841 attcagcact tcccaggtaa agtagttggc tgagttcagc accatactcg ggcgacccgg
    27901 ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca ggccctggaa
    27961 gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccacggcg agaaggtgtc
    28021 gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca ttccatagcg
    28081 ttttagcgcc agccagaaca tgcccacaat ccccagcacg cccgccgccg ccagcatcca
    28141 caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat
    28201 attggcgcga gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac
    28261 gaggaaaaac agccacgctt tgctatcctg gcgcagaaag tagaccacca gcatcgccgg
    28321 gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg agaagatctg
    28381 actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct
    28441 taccagcgcg atgcccatca ggatcaccca cgtaagattg gtctccacgc ggttcatggt
    28501 aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac
    28561 atagtaaacc gcgtagaagc agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg
    28621 cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa
    28681 ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac ggcgaaactc
    28741 aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagacaacaa acaggccact
    28801 gaattgcagc agactcatgc gacctccccg gcggcaatcg ccaacgcccg ctgccagcct
    28861 tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccaccgacgc caactggcgc
    28921 tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac cggcaaatgc
    28981 tgttccgtca tatcctgcca gaacggattt tcccggttaa gcacacaaag aatgcccgcc
    29041 tgaatcagta agcaaagcgt accaatgccc tgctggcggg caaaaataaa gtaaccaaga
    29101 tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct caaaatttgc
    29161 agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg
    29221 ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga
    29281 acggcgcaca aggcagcaat atggtcattg ctgcggtcac cggagttccc caccagaatg
    29341 gtcattttcc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg gtccatccgc
    29401 gtcgggaagt acagcagttc gccccgcacc tttggatgcg ttttggcaaa aaagctcaaa
    29461 tcgccgcggg tggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa
    29521 aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca gatatgccag
    29581 aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac
    29641 tgaccgtgga agaaaaaacg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc
    29701 gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag
    29761 ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc ggccagcgcg
    29821 tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga
    29881 atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat
    29941 agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta
    30001 gccagtgggc aaataccatc aataaagtga actggctgac ttccgccaga atataaaacc
    30061 gcagtgacgc tttggcgatc accagataac caaagacata agcgcccact tttaacacat
    30121 cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca
    30181 gccagatagc aaaatcacgc agcagccaga cggtaaaact cgccgccgcc actgccggta
    30241 agacgaattt cagcgattta accacttccc gggtgatatc gcgcttttcc gttagccgcg
    30301 acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg
    30361 aaatactgct caccccttgc cagatcccca cttcatccca gctatactgc gccgccagca
    30421 gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg
    30481 taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga tagcttaacg
    30541 ggatgacacc acgtttgatc aacatgatgg cggcaggaat taccaccagc gcgggaatca
    30601 gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcga
    30661 gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca tcgcgaaagc
    30721 ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc agcgccacta
    30781 aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt ggcgcagctg
    30841 ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct gatgaagtgc
    30901 cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat ttggttacac
    30961 cgttaaagat gccagctccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg
    31021 ccgccagccc aagccccgcc ggaccaaatg acacagccag caacttaccg accagtaacc
    31081 cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca
    31141 tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg
    31201 ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta gcgatcttca
    31261 ccgtggaact caccaaagcg ttcccccgca gggcaaccgt gcagcggaat gtaatggaac
    31321 accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc
    31381 cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc
    31441 tcgatacgcc cggctttcgc cagaggcgct aacgcatcgt agtagttttg ccacagcgcc
    31501 agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta cgcagcttgc
    31561 agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc gacctgacca
    31621 cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg ttcgattaac
    31681 gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtatagtt tttggtttca
    31741 tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac
    31801 atcacgccct gagcggcatc ttctaccaca aacagattat gcttttttgc caacgccata
    31861 atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg
    31921 tcggtgatcg ccgcttcaat cagcgtttcg tcgatgttca tggtgtccgg gcgaacatcc
    31981 acaaaaacga tttttgcgcc acgcagcaca aaggcattgg cggtggagac aaaggtgtag
    32041 ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc
    32101 gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac
    32161 tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac
    32221 tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca
    32281 cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag
    32341 cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc
    32401 ccagtttagc gccgtttgca tcagctcagc acctacaccg cgtccagcca gcaggccaat
    32461 tcgcgcatct gtcgcattga gttcccgtaa agagacataa ccgcgaatac cgccggacgc
    32521 cgcacgtaaa atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg
    32581 tgcataaaag cgtctgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc
    32641 aaatgcggcg ctggctaact gacgtaatgc gggaatatcg gtctcttgtg ccactacagc
    32701 accactatca ctgacattgt tcacgggtag tgccaaatca acttcacctt ctaccaggga
    32761 gaatcccagc tgttgcaggg catccagttc acccgtattt gatgccgcaa ttttggcctg
    32821 cacccgtgac cacggcgcta acacgtctgg cgtcaggagc ggtgcttcag acgtaatgcg
    32881 cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc
    32941 gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc gctaatgaac
    33001 tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg atctcttcca
    33061 ggcaggcaat cttaaagccc tggcgttttt ctactgtctg cacaaaggta ctggcttcaa
    33121 tcaggctgtc gtgagtgcca gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg
    33181 tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccacgct
    33241 ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagcccgg
    33301 tcaccgccca gtttgacttc ggctgttttg gcttttcttc cagcgagata gcgcggaaat
    33361 tgtcgtcaaa ctccaccacg ccaaagcgtt ccgggtccat cacctgatag ccaaataccg
    33421 tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga
    33481 agatgttatc gcccagcacc agacaagaag gttcaccgtt gaggaaggtt tcaccgatga
    33541 taaaggcctg cgccagaccg tccgggctgg gctgttcggc gtattccagc tggataccga
    33601 actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc gtagtgatga
    33661 tgagaatttc gcggataccg gccaacatca gcaccgacag cggatagtaa atcattggct
    33721 tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc
    33781 cggaaccgcc cgccaggata atacctttca tgtttgcctc cgctgaaaat tagcctttca
    33841 gacctaaacg ctcgccctga tagctgccgt cctgcacctg cttccaccag ctttcattag
    33901 ccagatacca ctgcaccgtt ttacgcattc cactttcaaa ggtttcctgt ggcagccaac
    33961 caagttcacg ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt
    34021 cagcgacaaa ggtgatcaag tcacgatagt gcaccacgcc gtgcggcttg ttcggagcca
    34081 gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac
    34141 caccaatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg
    34201 cgtgatcttc cacatacagc cagtcacgga tttgctgccc gttgccatat accggcagcg
    34261 gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag
    34321 gaccgtagtt attcgagcag ttggtgatca gcgtcggcag accgtaggtc cgcagccagg
    34381 cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc ggcgcatacg
    34441 gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc accatatact tcgtcggtgg
    34501 agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgtattc cagtaagccc
    34561 gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc
    34621 cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat
    34681 gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa
    34741 agtgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat
    34801 cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa
    34861 taaacccggc accacctgtt atcagaattt ttctcatcag cgccagactc ctttggcatc
    34921 gacgacatac tgctgatgga cattgtcgcc attgataact ttgaactgac tatgatcgac
    34981 cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag
    35041 cccggtcagt tttttcggca actggtggat gttaggctca acaaccagcg tttcgccgct
    35101 gtgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat
    35161 attcggttta aacgccagac caaagcaggc gattttcagt tcactggcgc gtttatcggt
    35221 agcagcaagg cagtcggcaa ccgccgcttt cacctgatcg ataacccaga acggtttgtg
    35281 atcgttaact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct gtgccacgat
    35341 aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct gaagaatatt
    35401 aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc cctgatcggc
    35461 acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt
    35521 gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat
    35581 tttgtacagt tcgctggcgc gggtcgaaca aaccggcgtc ataccaccaa tcacgcgatc
    35641 gtttttaatc agctcgacca taacctggcc cggtaacacg cgttccgggc agtaagcaat
    35701 gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtg agatccggac gcatctctgc
    35761 taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa ggatcaccag
    35821 cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc
    35881 tggctcatga tcgcctttaa acggcgtggg tacagcaatc agccaggcat ccgcttcaac
    35941 tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa
    36001 atcaggctcg acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat
    36061 atcgacacca atcacctgtt tttgccgtga ggcaaacgct gctgctgtag gcagcccgat
    36121 gtaacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt
    36181 aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc
    36241 atagtttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac aattcgctgc
    36301 ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg ctcagtggta
    36361 tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag
    36421 tcggtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga
    36481 tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc tctgacgttc
    36541 gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc
    36601 agcgcgtggc agatttcttc aaagccacga ccgaaacttt cacgcctgtg accggtcacc
    36661 agaatcatct ttttatcggg gtcgataaac gggtaatttg ccgccagttc tgaacgcagc
    36721 gtgtcgctgc tcatcacctg atcacgcccc cataacagtg catcaatgac tgtattacca
    36781 gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg ggaagtttcg
    36841 gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc
    36901 cacggcgaat agagatcgcc cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc
    36961 tgataaaacg ccgccaggct ggttgccagc gtcgttgttg tgtcgccgtg aaccagcacg
    37021 acgtctggtt tgaactcggc aagaataggt tttaaccctt ccagaatccg acaggttatc
    37081 tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag
    37141 agtttcagca cctgatcgag catctcccga tgctgcgcag tgacgcaaac tttagcctca
    37201 aaaaaaggat cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc
    37261 gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt
    37321 gcgctcgccg cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc
    37381 gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg
    37441 ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagagt
    37501 tggaccaaca ttcagggtgt ttaacatggc ccgattctga tcatagtcga gatcaaaggc
    37561 cggaccgacg gcctgtaaat tttccagtcg agcctggagc attggacgcc caagcaggaa
    37621 catttctgaa tcaggtaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg
    37681 ctgctcagca attttcagcg cctgctcaat gctgttcatc cggcggtcgt agatggcttt
    37741 cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc
    37801 gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg
    37861 acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc tgtcattgac
    37921 cgcgcgggta aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc
    37981 cgcatcggct ttgctgttgc ccaccatccg ctgtttgtaa tagtcggttt gcagccagaa
    38041 ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat
    38101 gaccgatggt tgatcggcag aagccatgtt tgaacggaca tccaggttac gcaaaaattg
    38161 ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcga
    38221 gctccactcc tgacgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagccc
    38281 catgccaata atccatagct tcccagccca caaggtacga aacaacccac gaatatccag
    38341 ttcattttca gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca catcctcatt
    38401 tatttggtta aattggggct gccaccacga tttctacgca ggctgcgttt tacgcgctta
    38461 ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc
    38521 aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacgcca
    38581 atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg ggaagtaaac
    38641 ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg gctcatgcct
    38701 ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac
    38761 aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag
    38821 ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga
    38881 cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaagcac
    38941 cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag
    39001 acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg
    39061 gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag
    39121 gagccaaaga tataacccag gctactgaga taaagtttgc cgaacaccat cataacaatg
    39181 ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg gtcatccagc
    39241 gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg cggaatatag
    39301 taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga
    39361 ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc gacttttttt
    39421 gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca
    39481 gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat
    39541 aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag ttctgtgcaa
    39601 aaattaacca tttgatatgc aaattgcata acatgccagc aaattccagg aattttccta
    39661 acatcgccag cgcggcatta agattacaga tataaaagca aaacgccacg taaacacgtg
    39721 gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg
    39781 tcttggtcat ggccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt
    39841 gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg agcagctctt
    39901 ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct
    39961 tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt
    40021 ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg ccgccctctt
    40081 ccacgttacg cgccgcaccg aagaagcgtt tcggacgatg aagggcattg gcgtccacac
    40141 caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc gccagacgag
    40201 tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt ttggccttct
    40261 cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa
    40321 caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca
    40381 gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca
    40441 gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga ccacgaccga
    40501 taggtgatgc cagatccagt acgcgagcgg ttaaatcttc agtagaaccg ttaccacgtt
    40561 ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc
    40621 gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt
    40681 caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac
    40741 ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg
    40801 cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca ccaaagatat
    40861 cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac
    40921 gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat
    40981 tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac
    41041 ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt
    41101 cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg
    41161 acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca
    41221 agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac
    41281 gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt
    41341 agcgtcgagg aactctttca actgaccttt agacagtgca cccactttgg ttgccgccac
    41401 ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt tcggcgcagt
    41461 gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc
    41521 gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc
    41581 gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg
    41641 aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttcgttg
    41701 ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag
    41761 caatagtaag ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt
    41821 ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa
    41881 ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg
    41941 gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct
    42001 tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccacgtgcct taattatggc
    42061 accgacgcgt gaacttgccg tgcagatcca tgccgacgca gaaccgctgg cggaagctac
    42121 cggcctgaag ctgggtctgg cttacggtgg tgatggctac gacaaacagc tgaaagtgct
    42181 ggaaagcggc gttgacattc tgattggcac tacggggcgt ttaattgact acgccaagca
    42241 gaaccacatt aacctcggtg ccattcaggt ggtggtactg gacgaagccg atcgcatgta
    42301 cgatctgggc tttattaaag atatccgctg gctgttccgc cgtatgccgc ctgcaaacca
    42361 gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga
    42421 gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat
    42481 taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat
    42541 cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc gttgtgaaga
    42601 gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc
    42661 gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg atattctggt
    42721 tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg tctttaacta
    42781 cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg
    42841 cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat
    42901 tgagacctat attggtcact caattccggt aagcaaatac aatccggacg cattgatgac
    42961 cgatctgcca aaaccgctgc gcctcacgcg cccacgtaca ggcaatggtc cgcgtcgtac
    43021 tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc
    43081 aaactgtatc aagatggcaa gagaatgaat cccgatgagc atactgtagt aagtgattca
    43141 ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct
    43201 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    43261 aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt ctggctgctg
    43321 gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc
    43381 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    43441 cgacattacg tcttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    43501 gttgtccggt acaggtgatc agcggtgaag aggaagcacg tctgatttat cagggcgttg
    43561 ctcacacaac tggcggtgcc gatcagcgcc tggtggtgga tataggcggt gccagtactg
    43621 aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg
    43681 tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat tttgatgctg
    43741 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    43801 ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    43861 aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag cgagccattc
    43921 attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt gcgttagtgt
    43981 tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt cagtgtatga
    44041 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg
    44101 agcaggatat tcgcagccgt acgctgcgta atattcagcg ccgctttatg atcgatattg
    44161 atcaggcgca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaaggaat
    44221 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    44281 gcctgagcgt tgactttaaa caagcgccgc aacacgctgc ttatctggtg cgtaatctgg
    44341 atcttcccgg ttttaccccc gcacagaaaa aattgctggc gacgctactg ctcaaccaga
    44401 ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccgccg cgcgtcgcag
    44461 aacaactctg ccgtttactc cgcctggcca tcatttttgc cagccgtcgc cgtgacgatc
    44521 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    44581 gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaagc cagtggcaga
    44641 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgacg
    44701 catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    44761 cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc gctcctgttc
    44821 ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg ggcgcaccag
    44881 ctcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    44941 aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga tgctgctctg
    45001 gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact ccagcccttt
    45061 tgacgcgtgc agagtcatca gttgcacctg atccagctct tcttcactct caccacgctc
    45121 catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    45181 ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    45241 catttcggcg gctttcgggc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    45301 gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc
    45361 caaccagtga gtaaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag
    45421 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc actcacccag
    45481 ctttttcagc gtcgccgggc caatctctct cttcggcgtg ttaacgatac gcagaaatgc
    45541 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    45601 acgagagaaa aacgacgtac caccagatat tttgtatggg atgcggtttt gcatcaggaa
    45661 tttttcaaac acccgcgact gatggttacc gcgataaaga atggcgtaat ctttgtactg
    45721 cgttttattg acgaagtgat gggcgatcag ctcgccagta acgcgctcgg cttcatgttc
    45781 ctcgttattc gcgcttaaaa cttttagctc tgtgccgtaa cccagttcgg agaacagacg
    45841 cttttcaaag acgtgcggat tattggcgat caggatgttc gccgctttca aaatacgtcc
    45901 ggaagagcgg tagttctgct caagcttaat caccttcagc gccggaaaat cctgactcag
    45961 cagcaccagg ttttgtggac gtgcgccgcg ccaggagtag atcgactggt cgtcatcgcc
    46021 caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat actggctggt
    46081 gttggtgtcc tgatactcat ccaccagcag atagcgaatt ttgttctgcc agcgctcgcg
    46141 gacttcttca ttgcgttgca gcagcaacgt cggcaataaa atcagatcat cgaagtcgag
    46201 aacattgcac gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacggtc
    46261 ccgctcgcca atcgcactgg ctgccgcctg ggacggtgtt ttgagatcgt tcttccagtt
    46321 agagatggtc gaaatcaact gttgcaggag aactttgtca tcttcaatca gcccttcggt
    46381 caactctttg agcaacgcca gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat
    46441 ccccagcgcc gcatactcac gcttgatgat atccagcccc agcgtgtgga aggttgagat
    46501 catcagccca cgcgcctctt tgcgacccag cgtctgtccg acacgctctt tcatctcgcg
    46561 cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg
    46621 gatcagatgg gcgattttat tggtgatcac acgagtttta ccggaacccg cgcccgccag
    46681 caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat
    46741 aggtgtattg ctcaatcttc gaacgggggg aggattgtag cagaatcgca tttgaatgtg
    46801 accgcgtgta agccgctaac cggttggctg cggatgttta aatcagagag agaagaccga
    46861 aaccggagtt catcgtgaat ccattagact tagaaaatat cgggtcgtaa cgttgtgtta
    46921 ttactttttc agagtttact aaggagaggt gtactcgtgg cgattaattt cagtcctaag
    46981 gttggtgaaa tactggaatg caactttggt aactatcctg ttagccagaa tggacagttc
    47041 agcaccaagt attacgatgg ccgcattcct cctgaaatga taaaaaacag gttagttgtc
    47101 gtgctcaacg gaaaaattaa tggcaacgca tgtatcgtcg ttccattatc aaccactcgc
    47161 gatcatgaca agttaaatcg tggaatgcat gttgaaatag ccagtaatgt tatcaatggc
    47221 ctgcaattct ttgatcagca gattcgttgg gccaaagccg acctggtaca acaagtcagt
    47281 cgaaaccgat taaatagagc aaggacttac agagggtatc ttaatcaatg tttgccacat
    47341 gagttggttg cagacataca gagggcggtc atcaagtcaa tcaatgccat atcgctcata
    47401 aattgacaat aaaataacca tccccgataa tgactttgta gcccatagag gccctaagtt
    47461 ttctcccctt gagggacctg cgaagcccgg attttccggg cttttgccat atatgtacca
    47521 gcccctccct gactgacaat tacgttagca tctgcggatc atgctaaaat cgccgcctga
    47581 caattattca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta
    47641 aaagaagaga aactggctct ggatcttctc gagcagatta agaacggtgc cgatttcggc
    47701 aagctggcga agaaacactc catttgccca tcaggcaaac gcggcggtga tttaggtgaa
    47761 ttccgccagg gtcagatggt tccggcgttc gataaagttg tgttctcttg tccggtactg
    47821 gagccgaccg gcccgctgca cacccagttc ggttaccaca tcattaaggt gctgtaccgc
    47881 aactaatagc aaggccttct ccaggagaag gccttgagtg ttttctccct ctccctgtgg
    47941 gagagggtcg gggtgagggc atcagcgcgc acttaacccg caacagcaat acgtttcata
    48001 tctgtcatat agccgcgcag tttcttacct acctgctcaa tcgcatggct gcgaatcgct
    48061 tcgttcacat cacgcagttg cgcgttatct accgcgcctt ccggaatagc tttacccaag
    48121 tcgcccggtt gcagctctgc cataaacggt ttcagcaacg gcacacaagc gtaagagaac
    48181 agatagttac cgtactcagc ggtatcagag ataaccacgt tcatttcgta cagacgctta
    48241 cgggcgatgg tgttggcaat cagcggcagc tcgtgcagtg attcataata tgcagactct
    48301 tcaatgatgc cggaatcgac catggtttcg aacgccagtt caacgcccgc tttcaccatc
    48361 gcgatcatca gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc
    48421 ggcgcggttt caaacgcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca
    48481 tcgttggccc agtccgccat cataccggaa gagaattcgc cggagatgat gtcgtccata
    48541 tgtttctgga acagcggtgc catgatctct ttcagctgtt cagaaagcgc gtaagcacgc
    48601 agtttcgccg ggttagagag acggtccatc atcagggtga tgccgccctg cttcagcgct
    48661 tcggtgatgg tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc
    48721 tcttccacca gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg
    48781 gtttgctcac ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc
    48841 gcacggtgac caccggttgc tgccgcccat gctttagcga tcgccatacc ttcgcctttc
    48901 ggatcgtttt ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct
    48961 tcacgcactt cggtgccagg gcatttcggc gcaaccatga ctacggtgat atctttacgg
    49021 atctgctcgc ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct
    49081 ttcatcagtg gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta
    49141 accaccagat ccgcctgcgg gatcagttct tcgtaagtac caactttaaa accgttttcg
    49201 gtcgctttac gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaagaa
    49261 atatccagac cagaatcacg catgttcagg ccctggttca gaccctgtgc gccacagccg
    49321 acgatgacta cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc
    49381 ataaagcgac atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag
    49441 ttagccatgg tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga
    49501 ttgaattcac tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat
    49561 tgcaattttt gcaacgtcaa catcgagggc tgcgccattg gatttacgcg atctgaaaac
    49621 cttcctgcat ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag
    49681 cccatccacg ctctcacggc agattcagcg cctggaagaa gatcttggtc agccgctatt
    49741 tgtgcgcgat aaccgcacgg taacgctgac ggaagcgggc gaagagctgc gcgttttcgc
    49801 ccagcaaacg ctgttgcagt atcagcaatt gcgccacacc atcgatcagc aagggccgtc
    49861 gctctctggc gaattacata tcttctgctc ggtgaccgct gcctacagcc atctgccgcc
    49921 gattctggat cgcttccgcg cggaacaccc gtcggtggag atcaaactca ccactggtga
    49981 tgcggcagat gcaatggaaa aagtggtcac gggagaagcg gatctggcga ttgccgggaa
    50041 accggagacg ctacccggcg ctgtggcgtt ttcgatgctg gagaatctgg cagtagtgct
    50101 gattgccccc gcgctgccct gcccggtgcg taatcaggta tctgtcgaaa agcccgactg
    50161 gtcaacggtg ccgtttatta tggccgatca ggggccggta cgccgccgca ttgaactgtg
    50221 gtttcgacgc aataaaatca gcaacccgat gatttacgcc acggttggcg ggcatgaagc
    50281 gatggtatcg atggtggcgc tcggctgtgg tgtggcgctg ttgccggaag tggtgctgga
    50341 aaacagcccg gagcctgtac gcaaccgggt aatgatttta gatcgcagcg atgagaaaac
    50401 gccgtttgaa ctgggcgtgt gtgcacaaaa aaagcggctg catgagccgc tgattgaggc
    50461 gttctggagg attttgccga accacaaatg acgttgtcgc gcgggtaggc ctgataagcg
    50521 aagcgctatc aggcattttt ccctaacccg ccaaaaagaa cctgaacgcc ggattattgg
    50581 tttcgtcgtg gcaatcgtag cccagctcat ttagccgggt ttcgaaatcc ggttcatggt
    50641 cgccgagttc gaacgccgcc agtacgcgcc cgtagtcggt gccatggctg cgatagtgga
    50701 acaaagaaat gttccagtgc gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg
    50761 attccgggaa ttcgaagctg tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga
    50821 ccatatagcg cacatgcaac ttcgccattt cgtcgtcgga gagatccacc acgctgtagc
    50881 cgccgtcgtt gagcatctgc aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca
    50941 cgccgacaaa gatgcaggcg tttttggcat cggcaaaacg gtagttgaac tcggtgaccg
    51001 atcgcccgcc aagcagttgg cagaatttga ggaagctgcc tttttcttcc ggaatggtca
    51061 ccgccaacaa cgcttcacgc tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc
    51121 cgtggaagtt cacgttggca ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt
    51181 gctgggcgat atattttttc attcccgcca gcgccagcgc gccagagggt tccgccaccg
    51241 cgcgcacatc ttcgaataaa tccttcatcg ccgcacagat cgcatcacta tcgacggtga
    51301 tgatgtcgtc gagatactcc tggcataaac ggaaggtttc gtcaccgatg cgttttaccg
    51361 ctacgccttc agcaaatagc cctacgcgcg gcagatcaac cggatgaccc gcatccagcg
    51421 ctgctttcag gcaggcggag tcttccgctt ctacggcgat cactttgatt tgtggcatca
    51481 gttgtttgat cagcaccgca acgcccgccg ccagaccgcc gccgccgact ggcacaaata
    51541 cgcggtcgag atgggcgtcc tgctggagca gttccagcgc cagcgtgcct tgcccggcaa
    51601 tcaccatcgg atggtcgaac ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga
    51661 tcgctttagc tttcgcttcg tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc
    51721 cacgtaccgc atcgactttg atgtcggcgg tggcggttgg cataacgatc agggccttca
    51781 cgcctaaccg cgcagaagaa aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga
    51841 tcacgccgtg cgctttttgt tcttccgtca ggcccgccat catggcgtat gcgccgcgca
    51901 gcttaaagct gtgcactggc tggcgatctt cgcgcttcac cagaatgacg ttatcaagac
    51961 gcgacgacag tttttccatt ttttgtagcg gcgtaacctg cgccgcctcg taaaccggcg
    52021 cgcgcagcac tgctcttaaa tattcggcac cttccggagc accggacagg ggttgcgagt
    52081 cagccattat taacccccca gtttcgattt atcgcgcacc gcacctttgt cggcgctggt
    52141 tgccaggctg gcgtaggcgc acagggcaaa ggaaacctga cgttcacggt ttttcggcgt
    52201 ccaggctttg tcaccccgag cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct
    52261 tacctgtaac tgaatgccac ggttcgggat gtcgatagcg atcaggtcac catcttcaat
    52321 caggccaatg ctgccgccgc ttgccgcttc cggtgagacg tggccgatgg aaagaccaga
    52381 ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag gctttaccga gacccattga
    52441 tttcaggaag ctggttgggt agagcatttc ctgcatgcca gggccgcctt tcgggccttc
    52501 atagcggatc accaccacat cgcccgcgac aactttgcca ccgaggatcg cttctaccgc
    52561 gtcgtcctgg ctttcgtaca ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac
    52621 gcccgcggtt ttaacgatgc agccgttttc tgcgaaatta ccgtagagca ccgccaggcc
    52681 gccgtctttg ctgtaggcgt gttccagcga gcggatacag ccattggcgc gatcgtcatc
    52741 gagagaatcc caacggcaat cctgcgagaa tgcctgtgtg gtacgaatgc ctgcaggacc
    52801 tgcgcggaac atatttttta ccgcgtcatc ttgggtcagc ataacgtcgt attgttccag
    52861 cgtttgcggc aacgtcaggc caagtacgtt tttcacatca cggttcagta accccgcgcg
    52921 atccagttcg ccgagaatac cgataacacc accagcacgg tgaacatctt ccatatggta
    52981 tttctgggtg ctcggcgcaa ctttacacag ctgtggaacc ttgcgggaaa gcttatcgat
    53041 atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc gccagcaggt gaagtacggt
    53101 gttagtcgat ccacccatcg cgatatccag cgtcatggcg ttttcaaacg ccgctttgct
    53161 agcaatattg cgcggcagtg cactttcgtc gttttgctcg tagtaacgtt tggtcaattc
    53221 aacaatgcgt ttaccggcat tgaggaacaa ctgcttacgg tccgcgtggg ttgccagcag
    53281 cgagccgttg cccggctgcg acaggcccag cgcttcggtc aggcagttca ttgagttagc
    53341 ggtaaacatc ccggagcagg agccgcaggt cggacacgcg gaacgttcaa cctgatcgct
    53401 ctgggagtca gaaactttcg ggtctgcgcc ctggatcatc gcatcaacca gatcgagttt
    53461 gattatctga tcggacagtt tggttttccc ggcctccatc gggccgccgg aaacaaagat
    53521 caccggaata ttcaggcgca gggaagccat cagcatcccc ggggtgattt tgtcgcagtt
    53581 agagatgcag accatggcgt cggcgcagtg ggcgttgacc atatactcaa cggaatcagc
    53641 gatcagttcg cgagatggaa gtgaataaag catccccccg tggcccatgg caatcccatc
    53701 atccaccgca atggtgttga actctttggc aacgccgcca gccgcttcaa tttgttcggc
    53761 gaccagttta ccgagatcgc gcagatggac gtgacccggt acaaattggg tgaacgagtt
    53821 cacaaccgcg ataatcggct tgccaaaatc ggcgtcggtc attccggtgg cgcgccacag
    53881 cgcacgagca cccgccatat tacgaccatg agtggtggtg gcggaacggt acttaggcat
    53941 actttattta ctcccagtgt ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt
    54001 gtatttattg attaacttga tctaaccagc cccatttatc ttcggtttcg ccagtgaaga
    54061 ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc acaacggcct tcgccaacct
    54121 gaataccatc tacactgcgc actggcgtga tttctgccgc agtaccggac ataaacactt
    54181 catccgccag gtacagggat tcgcgcgaca gaacctgctc acgcacttca attcccagct
    54241 ctttcgccag tttgatgatg gcatcacggg taatacccgg cagcgcggaa gaggtgaacg
    54301 gtggggtgaa cagcacacca tctttcactt caaacaggtt ttcgcctgcg ccttcagaga
    54361 tataaccgtt cacatccagc gcgatacctt cctgataacc gtggcggcgc gcttcgctac
    54421 ccaccagcag ggaagagaga tagttaccac cggcttttgc cgcggttggg atggtgtttg
    54481 gtgctgcgcg gttccaggag gaaaccatcg catcgatccc ctgctccagc gcttctgcgc
    54541 ccagatacgc tccccacggg aaagcggcga taatcacatc ggttgagtat cccgctggcg
    54601 ggtttacacc catgccaaca tcaccaacga agatcagcgg acggatatag gcgctggtga
    54661 gattgttttt gcggatcacg tcacgacaag cttccatcag ctcatcaatg ctctgcgaaa
    54721 ccgggaagcg atagattttg gcggagtcat gcagacgctg catatgctca cgatggcgga
    54781 atacaaccgg tcctttgtgc gagtcgtagc aacggatgcc ttcaaaaacc gaggtgccat
    54841 agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc ccagcgaacc atctccccat
    54901 tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat attccttttg cgctcaggcg
    54961 cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt
    55021 ttatttaact gactaaacag taagtcgacc gaccgtgggc tggcaacggt caattcgata
    55081 tttatatttt gtgcatcgct ggcggcggcc atattcattg agcagacgtg gaaaccacga
    55141 tgacgcacca cgcgtaaaac acgttctaag gtttctggat tgaagcgagc cgatacattg
    55201 acctgatgtt gcatcatgat aatttctcca acatttctga attactggcg ccaggcggca
    55261 ccagcggcca gacgttctca agttcgtcga ttgagacatg aagcaggtat ggcccatcac
    55321 tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt acgggtgatg tgttggccag
    55381 ggatgccgaa ggcgctggct aacatgagga aatcggggtt atcagtaagg gtggtttcgc
    55441 tgtatcgttc ctgaaaaaac agttgctgcc attgtcgaac catccctaac cgttggttat
    55501 cgagtaagac gattttcaac ggtaactgct tgcgttttac ggtgcccagc tcttgcacat
    55561 tcatcatgaa agagccgtca ccggagatac agacaacggt atcgttcggt cgcgcgactt
    55621 gtgcgccaac cgccgccggt aaaccaaaac ccatggtacc taagccgctg gaggtgatga
    55681 aattttccgg gcgagtgtgg gcgatgtgct gcgcagccca catctggtgc tgccccacat
    55741 ctgtggtcac gacgcaatcc gcaggtttac gatccgacag ttgttttaac aacaacggcg
    55801 cgtagatagc gtcaccggga tggtcgtaac gccaggaatg ttcatcacgc agctgcgcgc
    55861 agtgttgctg ccagtcattg atatttaacg gctgctgtaa tgctggtaac agagcattta
    55921 aatcaccttg taatgccaca tgtgcctgac gcagcttgtt catttctgcc gggtcgatat
    55981 ccatatggat aacactggcg tgtggcgcga aggtgttcag tttgccggtc acccggtcat
    56041 caaaacgtgc acccacggcg atcagcaagt cgcactcctg caccgcgaag ttcgccgctt
    56101 tggtgccgtg cattcccagc atgcccagat agtacggata atctgcttca actgcgccca
    56161 gccctttcag cgtgcaggtg gcaggcattt ttgtggtagc gagaaattct cgtaaagcag
    56221 gaactgcctg cgccataccc acgccaccac caacgtacag catcggtttt tgcgcttttg
    56281 ccagcatctg gcgcgcttgc tcaacttcgg catgggggaa agtcacttcg ttttcaacgg
    56341 tggtgaacca cggttccaga tcgccgctgg ctaactggat atcttttggg atatcgacca
    56401 gaaccggacc aggacgacct gagctggcaa cgtcgaatgc ttcagccatg atgcgcggca
    56461 actcttccag cgactgcacc agaaagctgt gcttggtaca ggctaacgac aatcccagga
    56521 catccacttc ctgaaatgcg tcggtgccga taaacggtgc ggacacctga ccagtgatgg
    56581 caacgacagg gatggaatct aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc
    56641 ccggaccaga cgtggcgata catacgccag ttttaccggt agcacgggca taaccgatag
    56701 ccgccattgc cgcaccctgc tcatgtcggc acagcaagtg ctccacgccg ccgtcataca
    56761 atgcatcgta aaccggcata attgcgccac ccggataacc gaaaacggtg ttcacaccct
    56821 gtgcccgcaa cgcatgtacc acccactgtg cgccattcat agttagttcc ccgtcctgaa
    56881 tcttgagaaa cagaattttg tgctgttatt cattgtctgc tcctcggtta tgtttttaag
    56941 gtcaaaaaaa acccccggac ctttcggtgc gggggtctta gttcgttaag gcttgatctc
    57001 taagcctttc ctcgtccaag tgcagccccg cacggtggga taataatcac caccacgcta
    57061 atcacgacca ggctaatcac tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt
    57121 tcgaaggaat gcctaaagag ttaccatagg ttttgtaaat agtacaagat attttttggc
    57181 cgatttttat
//
LOCUS       DAZFAH010000009        56215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFAH010000009 DAZFAH010000000
VERSION     DAZFAH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.009"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..584
                     /gene="srlA"
                     /locus_tag="ACHJWB_000501"
                     /gene_synonym="gutA"
     CDS             21..584
                     /gene="srlA"
                     /locus_tag="ACHJWB_000501"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO4312184.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            581..1540
                     /gene="srlE"
                     /locus_tag="ACHJWB_000502"
                     /gene_synonym="gutE"
     CDS             581..1540
                     /gene="srlE"
                     /locus_tag="ACHJWB_000502"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO4312185.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1551..1922
                     /gene="srlB"
                     /locus_tag="ACHJWB_000503"
                     /gene_synonym="gutB"
     CDS             1551..1922
                     /gene="srlB"
                     /locus_tag="ACHJWB_000503"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4312186.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1926..2705
                     /gene="srlD"
                     /locus_tag="ACHJWB_000504"
     CDS             1926..2705
                     /gene="srlD"
                     /locus_tag="ACHJWB_000504"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4312187.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2810..3169
                     /gene="gutM"
                     /locus_tag="ACHJWB_000505"
     CDS             2810..3169
                     /gene="gutM"
                     /locus_tag="ACHJWB_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4312188.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3236..4009
                     /gene="srlR"
                     /locus_tag="ACHJWB_000506"
                     /gene_synonym="gutR"
     CDS             3236..4009
                     /gene="srlR"
                     /locus_tag="ACHJWB_000506"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4312189.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4002..4967
                     /gene="gutQ"
                     /locus_tag="ACHJWB_000507"
     CDS             4002..4967
                     /gene="gutQ"
                     /locus_tag="ACHJWB_000507"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4312190.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4964..6478)
                     /gene="norR"
                     /locus_tag="ACHJWB_000508"
     CDS             complement(4964..6478)
                     /gene="norR"
                     /locus_tag="ACHJWB_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4312191.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFNVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6665..8104
                     /gene="norV"
                     /locus_tag="ACHJWB_000509"
     CDS             6665..8104
                     /gene="norV"
                     /locus_tag="ACHJWB_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4312192.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8101..9234
                     /gene="norW"
                     /locus_tag="ACHJWB_000510"
     CDS             8101..9234
                     /gene="norW"
                     /locus_tag="ACHJWB_000510"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4312193.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9362..11614)
                     /gene="hypF"
                     /locus_tag="ACHJWB_000511"
     CDS             complement(9362..11614)
                     /gene="hypF"
                     /locus_tag="ACHJWB_000511"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4312194.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKHVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11767..12294)
                     /gene="hydN"
                     /locus_tag="ACHJWB_000512"
     CDS             complement(11767..12294)
                     /gene="hydN"
                     /locus_tag="ACHJWB_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4312195.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12443..13453)
                     /gene="ascG"
                     /locus_tag="ACHJWB_000513"
     CDS             complement(12443..13453)
                     /gene="ascG"
                     /locus_tag="ACHJWB_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4312196.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            13713..14894
                     /locus_tag="ACHJWB_000514"
     CDS             13713..14894
                     /locus_tag="ACHJWB_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO4312197.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVHSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPVTAVKYTYTVIPALVMTWCLSYIERWVDRITPAVTKNFLKP
                     MLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMH
                     RVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASA
                     IMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFTSVQFFD
                     PANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSL
                     N"
     gene            14903..16327
                     /gene="ascB"
                     /locus_tag="ACHJWB_000515"
     CDS             14903..16327
                     /gene="ascB"
                     /locus_tag="ACHJWB_000515"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4312198.1"
                     /translation="MSVFPQGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARIFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16441..16860)
                     /locus_tag="ACHJWB_000516"
     CDS             complement(16441..16860)
                     /locus_tag="ACHJWB_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4312199.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(16857..17168)
                     /locus_tag="ACHJWB_000517"
                     /pseudo
     CDS             complement(16857..17168)
                     /locus_tag="ACHJWB_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(17341..18102)
                     /locus_tag="ACHJWB_000518"
     CDS             complement(17341..18102)
                     /locus_tag="ACHJWB_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312200.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLNPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(18127..18597)
                     /gene="hycI"
                     /locus_tag="ACHJWB_000519"
     CDS             complement(18127..18597)
                     /gene="hycI"
                     /locus_tag="ACHJWB_000519"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4312201.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18590..19000)
                     /gene="hycH"
                     /locus_tag="ACHJWB_000520"
     CDS             complement(18590..19000)
                     /gene="hycH"
                     /locus_tag="ACHJWB_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4312202.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(18997..19764)
                     /gene="hycG"
                     /locus_tag="ACHJWB_000521"
     CDS             complement(18997..19764)
                     /gene="hycG"
                     /locus_tag="ACHJWB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4312203.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(19764..20306)
                     /gene="hycF"
                     /locus_tag="ACHJWB_000522"
     CDS             complement(19764..20306)
                     /gene="hycF"
                     /locus_tag="ACHJWB_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4312204.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20316..22025)
                     /gene="hycE"
                     /locus_tag="ACHJWB_000523"
     CDS             complement(20316..22025)
                     /gene="hycE"
                     /locus_tag="ACHJWB_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4312205.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22043..22966)
                     /gene="hycD"
                     /locus_tag="ACHJWB_000524"
     CDS             complement(22043..22966)
                     /gene="hycD"
                     /locus_tag="ACHJWB_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4312206.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(22969..24795)
                     /gene="hycC"
                     /locus_tag="ACHJWB_000525"
     CDS             complement(22969..24795)
                     /gene="hycC"
                     /locus_tag="ACHJWB_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4312207.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSTEGKLWFALGRL
                     GTVLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(24792..25403)
                     /gene="hycB"
                     /locus_tag="ACHJWB_000526"
     CDS             complement(24792..25403)
                     /gene="hycB"
                     /locus_tag="ACHJWB_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4312208.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25528..25989)
                     /gene="hycA"
                     /locus_tag="ACHJWB_000527"
     CDS             complement(25528..25989)
                     /gene="hycA"
                     /locus_tag="ACHJWB_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4312209.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26201..26551
                     /gene="hypA"
                     /locus_tag="ACHJWB_000528"
     CDS             26201..26551
                     /gene="hypA"
                     /locus_tag="ACHJWB_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4312210.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26555..27427
                     /gene="hypB"
                     /locus_tag="ACHJWB_000529"
     CDS             26555..27427
                     /gene="hypB"
                     /locus_tag="ACHJWB_000529"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4312211.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27418..27690
                     /gene="hypC"
                     /locus_tag="ACHJWB_000530"
     CDS             27418..27690
                     /gene="hypC"
                     /locus_tag="ACHJWB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4312212.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27690..28811
                     /gene="hypD"
                     /locus_tag="ACHJWB_000531"
     CDS             27690..28811
                     /gene="hypD"
                     /locus_tag="ACHJWB_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4312213.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            28808..29818
                     /gene="hypE"
                     /locus_tag="ACHJWB_000532"
     CDS             28808..29818
                     /gene="hypE"
                     /locus_tag="ACHJWB_000532"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4312214.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFIEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRNIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELTEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            29892..31970
                     /gene="flhA"
                     /locus_tag="ACHJWB_000533"
     CDS             29892..31970
                     /gene="flhA"
                     /locus_tag="ACHJWB_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4312215.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDMKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32007..32360)
                     /gene="ygbA"
                     /locus_tag="ACHJWB_000534"
     CDS             complement(32007..32360)
                     /gene="ygbA"
                     /locus_tag="ACHJWB_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4312216.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32392..32571)
                     /locus_tag="ACHJWB_000535"
     CDS             complement(32392..32571)
                     /locus_tag="ACHJWB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312217.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            32647..35208
                     /gene="mutS"
                     /locus_tag="ACHJWB_000536"
     CDS             32647..35208
                     /gene="mutS"
                     /locus_tag="ACHJWB_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4312218.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35314..35970
                     /gene="pphB"
                     /locus_tag="ACHJWB_000537"
     CDS             35314..35970
                     /gene="pphB"
                     /locus_tag="ACHJWB_000537"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4312219.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(36021..36788)
                     /gene="ygbI"
                     /locus_tag="ACHJWB_000538"
     CDS             complement(36021..36788)
                     /gene="ygbI"
                     /locus_tag="ACHJWB_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO4312220.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            36984..37892
                     /gene="ltnD"
                     /locus_tag="ACHJWB_000539"
     CDS             36984..37892
                     /gene="ltnD"
                     /locus_tag="ACHJWB_000539"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO4312221.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37889..39151
                     /gene="otnK"
                     /locus_tag="ACHJWB_000540"
     CDS             37889..39151
                     /gene="otnK"
                     /locus_tag="ACHJWB_000540"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO4312222.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSAGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39148..39786
                     /gene="ygbL"
                     /locus_tag="ACHJWB_000541"
     CDS             39148..39786
                     /gene="ygbL"
                     /locus_tag="ACHJWB_000541"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO4312223.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39791..40567
                     /gene="ygbM"
                     /locus_tag="ACHJWB_000542"
     CDS             39791..40567
                     /gene="ygbM"
                     /locus_tag="ACHJWB_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4312224.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            40656..42020
                     /gene="ygbN"
                     /locus_tag="ACHJWB_000543"
     CDS             40656..42020
                     /gene="ygbN"
                     /locus_tag="ACHJWB_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO4312225.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(42060..42296)
                     /locus_tag="ACHJWB_000544"
     CDS             complement(42060..42296)
                     /locus_tag="ACHJWB_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4312226.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(42307..43734)
                     /locus_tag="ACHJWB_000545"
     CDS             complement(42307..43734)
                     /locus_tag="ACHJWB_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4312227.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            complement(43734..44327)
                     /locus_tag="ACHJWB_000546"
     CDS             complement(43734..44327)
                     /locus_tag="ACHJWB_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4312228.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALRDMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44474..44881
                     /locus_tag="ACHJWB_000547"
     CDS             44474..44881
                     /locus_tag="ACHJWB_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4312229.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(45002..45994)
                     /gene="rpoS"
                     /locus_tag="ACHJWB_000548"
     CDS             complement(45002..45994)
                     /gene="rpoS"
                     /locus_tag="ACHJWB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4312230.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46057..47151)
                     /gene="nlpD"
                     /locus_tag="ACHJWB_000549"
     CDS             complement(46057..47151)
                     /gene="nlpD"
                     /locus_tag="ACHJWB_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4312231.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47336..47962)
                     /gene="pcm"
                     /locus_tag="ACHJWB_000550"
     CDS             complement(47336..47962)
                     /gene="pcm"
                     /locus_tag="ACHJWB_000550"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4312232.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(47956..48717)
                     /gene="surE"
                     /locus_tag="ACHJWB_000551"
     CDS             complement(47956..48717)
                     /gene="surE"
                     /locus_tag="ACHJWB_000551"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4312233.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48698..49747)
                     /gene="truD"
                     /locus_tag="ACHJWB_000552"
     CDS             complement(48698..49747)
                     /gene="truD"
                     /locus_tag="ACHJWB_000552"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4312234.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49744..50223)
                     /gene="ispF"
                     /locus_tag="ACHJWB_000553"
     CDS             complement(49744..50223)
                     /gene="ispF"
                     /locus_tag="ACHJWB_000553"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4312235.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50223..50933)
                     /gene="ispD"
                     /locus_tag="ACHJWB_000554"
     CDS             complement(50223..50933)
                     /gene="ispD"
                     /locus_tag="ACHJWB_000554"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4312236.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(50952..51263)
                     /gene="ftsB"
                     /locus_tag="ACHJWB_000555"
     CDS             complement(50952..51263)
                     /gene="ftsB"
                     /locus_tag="ACHJWB_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4312237.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51457..51780)
                     /gene="ygbE"
                     /locus_tag="ACHJWB_000556"
     CDS             complement(51457..51780)
                     /gene="ygbE"
                     /locus_tag="ACHJWB_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4312238.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(51830..52435)
                     /gene="cysC"
                     /locus_tag="ACHJWB_000557"
     CDS             complement(51830..52435)
                     /gene="cysC"
                     /locus_tag="ACHJWB_000557"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4312239.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52435..53862)
                     /gene="cysN"
                     /locus_tag="ACHJWB_000558"
     CDS             complement(52435..53862)
                     /gene="cysN"
                     /locus_tag="ACHJWB_000558"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4312240.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53864..54772)
                     /gene="cysD"
                     /locus_tag="ACHJWB_000559"
     CDS             complement(53864..54772)
                     /gene="cysD"
                     /locus_tag="ACHJWB_000559"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4312241.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55024..56061
                     /gene="iap"
                     /locus_tag="ACHJWB_000560"
     CDS             55024..56061
                     /gene="iap"
                     /locus_tag="ACHJWB_000560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4312242.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLVVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        13263 a        15325 c        14705 g        12922 t
ORIGIN      
        1 aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg
       61 ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt
      121 tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg
      181 aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctactg ttaccgtgca
      241 ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa
      301 agtacaaacc cagctactac gcggcggcct cttatagctg ccactcaatg aatggcctct
      361 tcccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc ggtctgacaa
      421 cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca
      481 atttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca
      541 ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcata ttcggatcga
      601 aaaaggaacg ggtggctggg gcggcccgct tgagctgaaa gccacgccgg gaaaaaaaat
      661 cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac agcttactgg
      721 ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc
      781 ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac gtattcccac
      841 cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg tggaagatat
      901 ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc
      961 ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa gcgatggttt
     1021 actggcgaag gtgggaatgg gcatggggtc cgccgttgcg gtgctgtttc aatctggtcg
     1081 tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg tctcggcgct
     1141 cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct
     1201 ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cactgctttc
     1261 acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca
     1321 gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg ccatcaacgc
     1381 gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac
     1441 ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg cgccgactgt
     1501 actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta
     1561 tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga
     1621 tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt
     1681 gccacggcga gttgaaaggt gaactccatc ctggtttgca atttacgctc gggcagcatc
     1741 gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg
     1801 tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag
     1861 gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt
     1921 aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aacattaggc gcgttcctgt
     1981 gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag
     2041 ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aagtatggcg tacggttttg
     2101 gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct
     2161 ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc tttatcagcg
     2221 acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt
     2281 gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga
     2341 tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat
     2401 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
     2461 tgcattcact gatgctcggt aacctgctga aatcgccgat gttccagtca ctgttgccac
     2521 aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
     2581 taccgctcaa acgcggctgc gattatcagg acgtgctgaa tatgctgctg ttctacgcca
     2641 gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt
     2701 tctgattaac agcggagatc cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc
     2761 tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta tggtatccgc
     2821 actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat
     2881 ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtgggccg
     2941 ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg atcagcagcg
     3001 catcgtcgac accttgttta tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc
     3061 cgcaattacc ggtatgcatg cgggtgattt acagcccgat gtgatctttc cccatgatcc
     3121 actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga
     3181 atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa
     3241 acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt
     3301 tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat
     3361 tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga
     3421 atccgatccg cctatcgatc ataaaacgct catcaacacc cacaagaaag agctgattgc
     3481 agaagctgcc gttagtttta tccatgatgg cgattcgatc attcttgatg ctggcagtac
     3541 cgttttgcag atggttcccc tgctctcgcg ctttaataac atcacggtga tgaccaacag
     3601 cctgcatatc gtcaatgcgc tatccgaact ggataacgaa caaactatcc tgatgccagg
     3661 cggaacgttt cgtaaaaaat cggcctcatt tcacggacag ctggcagaga atgccttcga
     3721 acatttcagt ttcgataaat tgtttatggg caccgacggc atcgatctca atgcgggcgt
     3781 aaccaccttc aacgaggttt ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt
     3841 gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct
     3901 tgaaagcgtc gataagctga ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct
     3961 ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga
     4021 acgcgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc
     4081 tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg
     4141 tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca cttgccagta
     4201 ccggcacacc ggcttttttt gtccatccgg cagaagcgct gcacggcgat ctggggatga
     4261 ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc
     4321 tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga
     4381 cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct
     4441 gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc
     4501 tggcgatggc ggtcatgcag gcgcgcggat ttaatgaaga agattttgcc cgctcccacc
     4561 cagccggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg cgccgtgacg
     4621 atgccatccc acaggtggcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc
     4681 gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta
     4741 ccgacggcga tttacgtcgc tggctggttg gcggcggcgc actcaccacg ccagttaatg
     4801 aagcgatgac gacaggcggc accacgttgc aatcgcaaag tcgcgccatc gacgccaaag
     4861 agatcctgat gaagcgcaaa atcactgccg caccggtggt agatgaaaac ggcaaactca
     4921 ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc ttcattccca
     4981 gacgtttcgc cagccgatgc aggttggcga cgtcggtttc cagcatccgc gcgcaggcag
     5041 cccagttgtg atgattttgt gccagcgcct gacgaatagt ttcacgctgg aacgcttctg
     5101 tcgcttcacg caggttttgc ttaacaacgg gcaccgccgc cacttctggc gtcggcaacg
     5161 tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg
     5221 ctctcgacag cactaccgcc cgatgaatag catgttccag ttcgcgcacg ttccccggaa
     5281 aacgatagtg ttgcagtaaa tttcgcgctc cggcacttaa taccacgcgg gagagcccca
     5341 gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct
     5401 cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaag tcagcgcgaa
     5461 atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat
     5521 cgacccgcaa actgcggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca
     5581 gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atccagaaac agagtgccgt
     5641 tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca
     5701 catgcccgaa caactcactt tccgccacac tttccggcag tgcggcacag ttgagataga
     5761 ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac
     5821 cggttccagt ctcaccgctg atcaggacgt tgagatcgga cgccgccaca atctcaatct
     5881 cttttttcaa ttgcgtcatg cctggcgaca agccaatcat ctgcgtctgt ttcaccgctt
     5941 caaacggcgc ggcatcgccc ggcagcatat tctggctttc cagttgttca atcagcaacg
     6001 cattgcttaa cgctcccgcc gccagcgcgg caatcagccg taactcttcg tcgctgaaaa
     6061 cattgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg
     6121 caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac
     6181 cgtcataggg atcgggcaat tcgctgtctg caggaaagcg caccacgtcc ccggcgcggg
     6241 caatcgcttc aagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg
     6301 ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg
     6361 catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac
     6421 caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct
     6481 ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat
     6541 tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc agataaaaaa
     6601 ctggcacgta atctgcaatt agcaagacat ctttttagaa cacgctgaat aaattgaggt
     6661 tgctatgtct attgtggtga aaaataacat tcattgggtt ggtcaacgtg actgggaagt
     6721 gcgtgatttt cacggtacgg aatataaaac gctgcgcggc agcagttaca acagctacct
     6781 catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca gccgcgaatt
     6841 tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg tgattaacca
     6901 tgcagaagag gaccacgctg gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc
     6961 gatttactgt actgccaacg ctatcgactc gataaatggt catcaccatc atccggagtg
     7021 gaattttaat gtggtgaaaa ctggtgacac gctggatatc ggcaacggca aacagctcat
     7081 ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg atgacttacc tgacaggcga
     7141 cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc atctgttcaa
     7201 cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg ccaatatcct
     7261 gacgccgttc agccgcctgg taacaccgaa aattaccgag atcctgggct ttaacttacc
     7321 agtcgatatg atagcgacct ctcacggcgt ggtatggcgc gataacccga cgcaaattgt
     7381 cgagctgtac ctgaagtggg ccgctgatta tcaggaagac agaatcacca ttttctacga
     7441 caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggaa ttgcggaaac
     7501 cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa acgaaatcct
     7561 gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga ataacgtgat
     7621 gatgccgaaa atcgccgggc tggtggagga gatgaccgga ttacgcttcc gtaacaaacg
     7681 cgccagtgct ttcggatctc acggctggag cggcggtgcg gtggatcgcc tttccacgcg
     7741 cctgcaggat gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc gaccagacca
     7801 ggacgctctg gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc
     7861 gccgctgccg cagagcacgg tgaatacggt agttaaagaa gaaacctctg ccacgacgac
     7921 ggctgacctc ggcccacgaa tgcagtgcag cgtctgccag tggatttacg atccggcaaa
     7981 aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc cggataactt
     8041 cctctgcccg gaatgctccc tcggcaaaga cgtctttgac gaactggcat cggaggcaaa
     8101 atgagtaacg gcattgtgat catcggttcg ggcttcgccg cccgccaact ggtgaaaaat
     8161 attcgcaaac aggacgccac tattccatta accctgattg ccgccgacag catggatgag
     8221 tacaacaaac ctgacctcag ccatgttatc agtcaggggc aacgtgccga tgaccttacc
     8281 cgccagacgg cgggtgaatt tgccgagcag tttaatctgc acctgtttcc acaaacctgg
     8341 gtgacggata tcgatgccga agcccgtgtg gtgaaaagcc agaataatca gtggcaatac
     8401 gacaagctag tactggcaac cggtgccagt gcctttgtcc cgcctgtgcc tgggcgtgag
     8461 ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat
     8521 gcccgacgcg tgttgattgt tggcggtggt ttgattggta gcgaactggc gatggatttt
     8581 tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcgtta
     8641 atgccaccgg aagtaagcag ccgcttgcag catcggttga cggagatggg cgttcatctg
     8701 ctgttgaaat ctcagttaca ggggctggaa aaaacggatt ctggcattct ggcaacgctg
     8761 gaccgccagc gtagcatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa
     8821 accgccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat
     8881 ctgcaaacca gtaatgacga tatttatgcg ctgggcgatt gcgcggaaat caacggtcag
     8941 gtactgccat tcctgcagcc gattcaactt agcgcgatgg tgctggcaaa aaatcttctc
     9001 ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac gccggaatta
     9061 ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcaaat taataccgaa
     9121 cgccagggaa tggtggctcg cggtgttgac gatgctgacc agcttcgcgc ctttgtggtc
     9181 agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa cattgccgat gtaggtgggc
     9241 tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca
     9301 tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt
     9361 tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca
     9421 gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga
     9481 gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca
     9541 gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca
     9601 gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc agccactgct
     9661 gccagaaagc ggcgagatcc agttgattgt ccaccagcgg catcgtcacc ggatgcgtca
     9721 ctccgtggca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct tcataactta
     9781 acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc ccacacgatg
     9841 acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc cagttttgct
     9901 gttgcacact tgctgtttca gagtaattct gccactccgg cacaaagcgc aggcactgcg
     9961 ccagcaggtt tcgccacggc tgcttcgctg ccaaatcgcc acccggaagc gccaccgcag
    10021 gcaagccgcc caggtgctgg cattcgcgat agttcacccg caggcactcg ccgccccaca
    10081 aagcgccgtt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat
    10141 ccagcggcca ctgatgctct gccagacacg ccgctgcgtg ggcatgatga tgcagcaccg
    10201 tttgcgtcgg cagattcatt tcgcgcgccc actggctaga gacatagccc ggatgcgcgt
    10261 catgcacaac gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc
    10321 gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt
    10381 gttcaccgcg caccaggcag aaggtgtttt tcagatccgc gccgagacac agcacaggcg
    10441 gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc cgcgaacggc
    10501 gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg atgtcgcggt
    10561 tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga
    10621 tagccggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct
    10681 gtaacagcaa atgctggagc gggttcgcag gcaacatcac tccgacttcg ttaaggccag
    10741 gggcgatatc atcacaaagc tcaggtacgt gttttttatc caccagcaca atcggcgcag
    10801 cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaact ttctgctact ggcaacatga
    10861 ctgccagcgg tttcgccggg cggtgtttgc gctcccgcag tgtcgccacc gcgttactgt
    10921 tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca attttgccca
    10981 tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttccgcatgt tcaccatgac
    11041 ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac
    11101 gatcgagcgg gtcgcggtac tctttgtcac aagccggaca caacggaaac gccgccatca
    11161 cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg ccgcagtggg
    11221 tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt tcggcaaggc
    11281 aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct cccgcgctct
    11341 ggcggatggt gaactcagtg ggcagttgtg accagatgta cggctcacgc tcgacgctat
    11401 caatacgcgc cagcggcggg cagtgctgat gcaattgaac aagaaacgtt tccgggtctt
    11461 cccgcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga agatttaatt
    11521 gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac
    11581 gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg
    11641 cgtaacccgc ccagccggac acgacagcgt cgcatccggc agtcacaggt cggcgatacc
    11701 gcccgctccg tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta
    11761 agatttttag aacatcagcg ccgtacggcg gcgtttttct gcactcagtt gttcaagttt
    11821 attacgatcg acacaaatca gcgcatgagt cgggcaagcc gccatacacg ccgggccgtc
    11881 ttcacgatgg ttgcacaggt cacatttatt ggcttcggct ttgtcagccc gtacattcag
    11941 ccccgcgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc catacgggca
    12001 agccacaacg caggttttgc aaccaatgca acgttcctgc atcacatgaa caaacccttt
    12061 atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg
    12121 gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt
    12181 ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc
    12241 acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt
    12301 ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt tttaattcac
    12361 tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga
    12421 cgatgacatg tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggtggatca
    12481 gttttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
    12541 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
    12601 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
    12661 ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa
    12721 ctaacgcgct aaatttagcc ccacgttcga gcaacgtttc taccccttcg gccccgctgg
    12781 cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgcca
    12841 gcgcgtcttt atacccagca agacgttcaa tgctggtggg ggaatccatc gagccggtaa
    12901 ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcatta aagctggtct
    12961 gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca
    13021 ttatcggctg actgtgcgcg tcaatgatgt catcaatctc atccacgctt aaaaaacgcg
    13081 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
    13141 cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc
    13201 gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta
    13261 ccagccccag cgtctgagta ctcttcgccg acagattgcg ggccagcaaa tttgggcggt
    13321 aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
    13381 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
    13441 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
    13501 gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata
    13561 aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat tttttgaaac
    13621 cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta
    13681 catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac
    13741 gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga
    13801 cgcgtttgcg ctttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca
    13861 tccccggcgt gctcggcgtg gtacatagtg acaaccagtg tcaggtgatt atcggcaata
    13921 ccgtttcaca agcctttcag gaagtcgtca gcctgctgcc gggagatatg cagcccgcac
    13981 agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcggggatc ctcgatgcgc
    14041 tgatcagcac catgtcaccg ctgatcccgg tcaccgcggt gaaatacacc tacacggtga
    14101 tcccggcgct ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta
    14161 caccggcggt gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc
    14221 tggcaatcct gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg
    14281 tttacaccat tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc
    14341 ctctgctggt aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg
    14401 ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg
    14461 gcggttcatc actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc
    14521 tggcagcggc ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg
    14581 cgatccgcct gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg
    14641 ttgccggtat ggcggggctt gccagccact caatggcagc gccggggtta tttaccagcg
    14701 tgcaattctt cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc
    14761 tggcggtggt gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg
    14821 aggaagcggc tgccgaggcg cgaaagcatc agagcgcaca accgaccgtc gccaaagaag
    14881 taagtcttaa ttgaggatga aaatgtcagt atttccgcaa ggttttttat ggggcggcgc
    14941 gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaaggac tgaccacggt
    15001 cgatatgatc ccgcacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt
    15061 tcagttgcgc gatgacgagt tttatcccag ccatgaggca acggattttt atcatcgtta
    15121 taaagaagat atcgccctga tggcagagat gggattcaaa gtgttccgta cctcaattgc
    15181 ctggagccgc ctcttcccgc agggcgatga actgacgccc aaccagcagg gcattgcttt
    15241 ttaccgttct gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg
    15301 ccacttcgat gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct
    15361 ggtggagttt ttcagccgct acgccagaac ctgctttgaa gcatttgatg gtctggtgaa
    15421 atactggcta accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg
    15481 tctggtgttt gaagaaggtg aaaatcaaga tcaggtgaaa tatcaggccg cgcatcacca
    15541 gctggttgcc agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt
    15601 gggctgtatg ctggcgggcg gcaacttcta cccttacagt tgcaagccgg aagatgtctg
    15661 ggcggcgctg gagaaagatc gggaaaacct gttttttatc gatgtgcagg cgcggggcgc
    15721 gtatccggct tactctgccc gcatatttcg cgaaaaaggg gtaaccatag ataaagcgct
    15781 gggcgatgat gagatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc
    15841 gcgctgcgcc tcggcggaga tgaacgccaa caacagcagt gcggcgaacg tggtgaaatc
    15901 gctgcgtaac ccgtatctgc aagtgagcga ctggggctgg ggaattgatc cactcggtct
    15961 gcgtatcacc atgaacatga tgtacgaccg ctatcagaag ccgctgtttc tggtggaaaa
    16021 cggcctgggc gcaaaagatg aacttgctgc caatggcgag attaacgacg actatcgcat
    16081 cagctattta cgcgaacata tccgcgcaat gggcgaagcg attgcagacg gcattccgct
    16141 gatgggctac accacctggg gctgtattga tttggtttcc gcctcaacgg gtgaaatgag
    16201 caaacgctac ggttttgtct ttgttgaccg tgacgacgca ggcaacggca cgctgacgcg
    16261 cacacgtaag aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gtgaagattt
    16321 agagtaagca acagtgccgg atgcggcgtg aacgccttat ccggcctaca tgtcgcacga
    16381 accaggaagg cccgataagc gcagcgcatc gggcaatctg gggtttggca tcagtcaaaa
    16441 tcaaagaaac cattccggct taacgccaaa tcgggctgaa agtgccttaa tgtgggatat
    16501 cgtcagggat cgttgtccag acaaaatctg actgaccagg gatttagagc cgatttcgtt
    16561 cttcaaatcc gcataggtca gattatgttg atcaatcagc gtgcgaagta acgccacgcc
    16621 aacaggcatt gccgcaacgg ctttgtcgaa ctctgcaaac tcttggttgt tgttttcata
    16681 ttctgcaatg cggctcgcca gaaagtcgat aagtggattg gtgtcatctt cttcaatcaa
    16741 atattcgacc agttccagtg cttcacgata atcatcttct gagtcactac ctcccagaaa
    16801 ggggactgca ttgaccagtt ctcttgttgc tttaacagcg cgtgcagcat tagcagtcat
    16861 tctttgttct ctctgtagta acgtgtcagt ttgtcatact cggcgtgatt cgttatgtgc
    16921 ttcacaaaga accgcttatt aacaaagttt atgtaggcaa tcacccttag gttgttccca
    16981 cctacatcca gaacgtacca tttgttgcga tatttaaaat tgtcgaggtt gggaaacgcc
    17041 gttcgcatct cttctggcgt agaaaaatcc gtttccttaa taacccgcta caatgcctga
    17101 agagccagag cgtcgttagg atatttacgc gcagactctt caaatggcgc tttagaaatg
    17161 atgtgcattt ttaagtttta acttccttat agtaagtaaa cagccacatc tgcgagttct
    17221 atccttatgt gttcggaaac tatagctcat caaatccaag ttcacaatat gtaaacactt
    17281 gtgcgcgata taattacccc caaaatcctc atagccactc tgcaaaaagt cagttctggt
    17341 tcacccctca agcacatccc cctttatcaa gcaacgctgc gtctgcgcca gatcttccgt
    17401 ttgcgggtcg cgcccggcga tggggttgag gacgagttta cggttggggg tataagtaaa
    17461 acgcgtgagc gcgggatagc ttaacttgcc aaaccagccg tgaacacgct gccatttggc
    17521 gtcttttacc tgatacagac tggttatcca gtaaccagaa ttaaactgca tatccagcaa
    17581 ttgcgtgtga ccattgcctt gcaaatccag caaatcatct acgccagttt tgcttgcggt
    17641 gtagaatccc ctgggttcaa agacacaggg acggccttcg cgcgtgaacg tcataagaat
    17701 taaatgtgca taaggagcca gaccattacc ggtacttggt aaccagataa ccagatcctg
    17761 aatgccgtta cgatcaagat cggcgcgata gagttgccct ttgcccgcta tgtttgccag
    17821 catacccaca ggcacagacc aggatttacc ggcgcgatct ttaccgttca attgccattc
    17881 actatcatca tttggcatct tttcaagcgt cacgccagca acgcccactt ttctaaaatg
    17941 ccaggtttgc tcggcatcat aaggcatggt caggagaggt ttcagcgccc gcttttgcgc
    18001 gtattcgctg tcactcattg gcagatattc ggtaaagctg gtggcctgtg caggcagcac
    18061 taagctcaat aaaaaagcgc acatccctgt tctgatattc atttcgctga attccctgat
    18121 ggagaattat tcttcttcca ccgctaactg cgcgaagccg ccattccctt cccagccttc
    18181 cagtcgttga taaacggttt ctaccgcatc tttaatcggc tgagtcatcg ggtagtaaaa
    18241 gccgacgata tccggctgga tgccgaggaa aatcacttcg ccaatgtctt ctttcaactg
    18301 gtcgataaga tagttcagcg gcatgttatg ggtagtcatc ataaacatct cggcgatatc
    18361 atccgggtcg atgatgcgga tttcgccggg gtttagcccc atatcagtag cgtcaacaat
    18421 cagtaaccgc gtagggcgca gttcacggat ggcgacgatg tcgttttccg gtgcactacc
    18481 gccatcaatc accacccagt tacctttcgg cgcggcggcg cacttttccg ccagcagcgg
    18541 gccagcgcca tcatcgccca tcatgctatt gccaacacag agtaaaacgt cagtcacgca
    18601 gtctcctcac catcaaatag atggcgtttt cctgatgaat atcatgcagc atacttaaca
    18661 gcattgtact ccattccttc tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt
    18721 tggccagcat cggcacatgg ttaatgtcga tgacgatctc gccatatttc ggtacgcctt
    18781 ccattttgcg gcgggcttcg ctgcccgcct ccagagtggc aatccatgcc agatattcat
    18841 cccatgggca ggtgagcgcc gcttccaggc aatcgataac cccaaggtgg tgaccaatcg
    18901 ccaggctgta atagaccacc tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa
    18961 atttacggct cagttgactg aacaccacct tttcactcat cggatacgcg cctcttcaac
    19021 aacatgattc agatggctga caatctcgtt cagacgcgga tcgttttccg cttccagcca
    19081 gcgtgcaacc tgttcttcgc cctgccctaa ctgtgtaagg taatcatcgg caatctgacg
    19141 accgtaacga taacccgcca ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg
    19201 caccatatcg ccatgcagaa tctcagccgg ttgttcatcc tgctcacccg ggccacgggc
    19261 gtgaattttc tgctcaagca ggccgagtgc cattgcaaag ccgtacagcg tggcggcagg
    19321 cgttggcggg caacccggaa tgtagacatc taccgggacg attttatccg taccgcccca
    19381 cacgcagtag agatcgtgga aaataccacc gctgttaccg caggcaccgt aggagataca
    19441 aattttcggg tccggcgcgg actgccacgc acgcagcgca ggggatcgca ttgcacgggt
    19501 gaccgcgccg gtaaacagta aaatatccgc atgacgcggt gaaggaacga ctttaatgcc
    19561 gaagcgttct gcatcaaaca gcggcgataa agtggcgaaa atttcgattt cgcagccgtt
    19621 gcagccgccg cagtccacgc ggtaaacata ggcagaacgt ttgatttttt tcagtaacga
    19681 cgccttcatg ctggcgatgg attcatccac cgtcatcggg accggaatgc cgttggcgtc
    19741 acgggggcct aataaattgc tcatcagatg gcctctttca tatggcgagt cagttcaata
    19801 cggtcggacg gcaccaggca tttctggcgc ttacattccg ggcaagtctc aaagctttcg
    19861 cggtggtttt ccgcgcggct gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc
    19921 tctttctgga cggcgaaagg acgattgcag acgcgacagc tgcacagcac aaaacgggac
    19981 tgttgcagga aatcttcttt cttccacacc gccagttcgt actcttgcga cagtttgatc
    20041 gctgccgtcg ggcagacttc ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac
    20101 tcccacgcca gctcaccggt ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag
    20161 gcattgacgc aggccgcaca gccgatgcac tgctgcgggt tctgctccgg cttaccacgg
    20221 aagtttttat caaccgcaat cggctccagc ggataagacg aggtcgccgt gccggttttg
    20281 atgacttttt tgataaaggt aaacatggcg attccttatt tcagcggcga gtttttacgc
    20341 tcaatgctgt aacgctcgag ttctttgtac ggcaccactt tgctcttttt cttacgcaca
    20401 tcgaccacgg tcatgcggtc ggtacaggag tagcaagggt ccaggctacc gataatcagc
    20461 ggcgcatcgg aaacggtgtt accgcgcagc atgtagcgca gggtcggcca gttcgcgtag
    20521 gtcgcggcac ggcagcgcca gcggtacagc ttctggttgt cgccggtcat gctccagtgg
    20581 atatcgtcgc cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta
    20641 aagccttcca ccatcagcgg accgcccggc aggttatcca gaccgtagtc gatcatgttc
    20701 agcgcggtat aaacttcgtt gatacgcact ttcagacggg aaataacgtc gcagccctgc
    20761 tcgctgtgga cttccattgg cagcaggcca taaccgacaa acgggtgatc ggcgcgagta
    20821 tcgcgggcgt gaccgctggc gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt
    20881 tccgggtcca gacgaccaat gccgacggtg cgctgttcca tgttcggcgt actcagcagc
    20941 acatccacca gctcctgcac ttcacgacgc atctgttgcg ccagctggcg ggtctggatc
    21001 atgtcatctt tcaacagatc gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta
    21061 cgcgcaccgg tgaggatctc tgccattttc atggaggttt cacgcacgcg gaagaactgc
    21121 ataaagccgg agtcaaagcc ggtaaagtgg caggcgagac cgaggttgag cagatgcgag
    21181 tgcaggcgtt ccacctccag cagaatggcg cggatcatct gcgcacgttc tggcacctga
    21241 atacccatcg cgttttctac cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc
    21301 ccgcacacac ggtcagagag gaaggtcact tcgttgtaac ccatacgggt ttccgccagt
    21361 ttttccatgc cgcgatgaac gtagaacaga cggtagtcgg cgtcgataat gttttcgcca
    21421 tcgacgaaca gacggaagtg gcccggttcg tcagaagtga cgtgcagcgg accaatcggc
    21481 acgacgttgt ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc agcatcggtg
    21541 gtcggcgccg gacgctgacg ataatccatg ctgtctttac gcagcggata aagttcatcc
    21601 ggccagtcat ccggcagcac cagacggcgt tcatccggca gaccaaccgg aatcaaaccg
    21661 tacatatcgc gcacttcgcg ctcgccccac acagccgccg gaacgcgcgg cgtcacggac
    21721 ggatactccg gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca cttagtgccc
    21781 ttctccatcg acagcacgta ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta
    21841 ccaaacagca ccgacagcca gccaccctgt ttgtagtaaa gaaactccac cacttccggc
    21901 aggtagttca cctttacggt gacagtcagc tgatctttgg tctgccaggc gtggtccagc
    21961 acgacgcccg gaaatgcctc attcagcgcg gcgagataat gttgacctaa tttttcttca
    22021 gacatgctca aactctcttt aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa
    22081 agccaaaccc agcccaggta atgcgcggag taatatcaag acgcagacgg gccatgctgt
    22141 tttcgaacag cgcgataacc aggacgccga ccaccagttt gacgatggca atcaccagcg
    22201 ccagcagcag tccaccggcg gtgaaggttt ccatttgtcc ccacggaata aacaccccga
    22261 cgaacatctg caacaccacc agctgtttca ggctgatacc ccatttcatg acgccaaagc
    22321 cgctaccgct gtattcggag agcggacctt cctgcaactc ctgctcggct tccgccaggt
    22381 cgaacggcag tttgcccatt tcgataaaag tggcgaacgc acaggcacaa agcgccagca
    22441 ccagcgggat gctctggctc agcggccagt gataaacggt gtcggtgatg ttgctgatgt
    22501 tggtggaacc ggcaacctgt gcggcaaccc acagaccaag cagcagcatc ggttcgacca
    22561 gcacgccaag catcgcttca cggctcgcgc cgatagcagt aaacgggcta ccggtatcca
    22621 gaccggaaat ggcaaagaag aaacgcgcga tggcaaagag atacagtaag gtgatcaaat
    22681 cgcccagttg cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca
    22741 gcatgacgcc caccatcaca tacggcgtca ggcggaacac ccagccggag gcatccgggc
    22801 caacgctctg gcggcccagc agtttgataa tgtcacgata ctcctgcaac acacccggcc
    22861 cacgacggtt atgtaagcgg gctcgcgcca cgcgggtaat accggagagc agcggcgcaa
    22921 cggcaaatag caccagcgcc tgaattaacg gatataaaac actcattctc aggctcctcg
    22981 tgaaacaata atcaccacca gcaccgccag ttcaaccagc gccatccggc ggaacagcaa
    23041 cgcactcccc tcgcactgcc agcccggcac cagagacacc ggattcagcc atttgcgtag
    23101 tttcagcacc ggcgcaaacg cctgtttcac cggcatggca aaaccgtgag cggtaatcac
    23161 cattgatttt tcgtggtcgt agccgcacac ccaggccgag ccgcgggaac gcgacggcaa
    23221 acgatcgcct ttgcaaatcg ccataatgat gaatggcagc agcgggcagg caatcagcag
    23281 caatgtgatc atgggttgag aaacggtggt gttagcaggc tccagaggca gaggtacagc
    23341 agcagagagc atcggcagca gccacggcgc agcaacaccg ccaattacgc agcaaatcgc
    23401 cagtgccact acgcttacgc tcatcaggag cggcgcacag gtggcgtttt cggcttcttt
    23461 ggtacgcggc gcgccgagga acgtgacgcc atagactttc gccatacaca tcaccgccag
    23521 cgcaccggta attgccagac ccacagcgag cagcggcccc agcagacggg caacaaacgc
    23581 gccactattg ctcagtttga agaaggattg atagataacc cattccccgg caaacccgtt
    23641 cagcggcggc agcgcagcca ttgccattaa cccgactaac atggcgatgg agataaccgg
    23701 cattttcttg ccaataccac cgagtttctc gatatcgcga tgaccggtac ggaaccagac
    23761 gctccccgcc ccgaggaaca gtacgctttt gaacaggcta tggttaagca gatggtacag
    23821 accaccgacc aggccaagag caatcagcgc cggttgttcg agcgcgatac ccgttacgcc
    23881 agcgcccagc cccagcagga taatgccgat attttccagg gtgtggtaag ccagcaggcg
    23941 ctggatgttg tgttccatca acgcatacag accaccgacg aacgcggtga tcatgccgag
    24001 caccagcagc gcgatccccc accacagcgg cgcgttacca cccagcagcg acagggttaa
    24061 aatgcccagc aggccaattt tcatgactac cgtagaaaac aacgcggcag ctggcgcaga
    24121 ggcgttagca tgtgcctgcg gcacccagcc gtgcagcgga ataatcccgg ccagcaggcc
    24181 aaagccaatc acaccgagca gccagatatc ggaaccgagc ggcagctgtt gcatacgcat
    24241 atccagcagg cgcagatcca gcgtgccata acgctgccac agcagccagc aagcaatcgc
    24301 cagcagcaca gtgccaagac gccccagcgc aaaccacagt ttgccctctg tgctgttgct
    24361 ggtaaggaac accgcacaca aggccatgac ttcagccatt accacgaaca tgccgaggtt
    24421 gctggcgatg acggcgcaga cggcggcagc catcaacata ttgatctgca agccgttgca
    24481 cttcacctgc gcgtggcgat gccagtcaat gttgtagagg ctgacaaaca gaccgcacag
    24541 accgagcgta atcagccaaa tcgcgttaag cggagagatt tgcacatcgt agcttaccag
    24601 cgacagcgca ccgctcacgc caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt
    24661 atacagacta ccaaccgcgc cgccaattcc ggctatccag ccacttagcg tcttttgaaa
    24721 agaaaagaga aatgccagaa cagcggcggc gacaaaccat gccacgccgc tattgatcag
    24781 ggaaattgcg ctcatttagc ctctccactt tgagcctgct gaaacaaggt gagatcgcca
    24841 aagtccgtgt taaaggtcag ctcacgctta cgtttgctga cgcgggcgat atcggtgtta
    24901 tccaccagat gcagggcttt agtcgggcac atccgcacgc aggccggacc ttgttcatca
    24961 aaactgcaaa ggtcacattt gacggcgatc gcgcgaatac ccggcaccca gtcaagcagc
    25021 gtactgatac gcgccggagc aggcggtgcc ggtggcgctt tcggggtatt ggcatttgca
    25081 ggaatatcca gcggacggct gccggaaaat tcaattgcgc caaacgggca ggcgatgccg
    25141 cacagcttgc agcttacgca caggctttca ttcaactgca cggccccatc aacgcgggtg
    25201 atggcgttaa ccgggcagac caccgcgcag ggtgcatctt cacagtggtg acagagctgc
    25261 ggcgcagatt ctttttcatt tagcatcact ctcaggcgcg gcattgattg caggccgtgc
    25321 tggcgatgcg tctctgaaca ggcggcctca caagtgtggc agccgataca gagcgtggag
    25381 tcagcaatta caaaacgatt caccaggcat tcctcaggtg attgtcattt ttgacgaaaa
    25441 catgccgatg aaatgtcatt ttcgacactc atcgacacgc ccatccccaa acaggcgtaa
    25501 cgcctgcaaa acgggcaaag cctcagctca tgctgccggg ctttgtccct ttaccagttg
    25561 gcttaaattc accggcacat tgttttcaat ggcggtgtat aagctgtcgt aaacgccagc
    25621 gattttttcg agatagtgtt ccagaacctg ttcgcgatcg cggatgttga ccttgccatc
    25681 aatcatgccg tcaatgctca gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt
    25741 tgtttcgacg taatactcga tggtgtggct gttaaagcca tccgccaggg tgacgtaaat
    25801 acgaaaatgt tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg
    25861 atgcaggcgc gctttcgata acgtgatccc ctgaaccagc gaattgcagt agatgtgcca
    25921 ctggtcctgt aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc ttatttccca
    25981 aatagtcatt gtcaggttac ccgtttaaca gagatgccag ctttaagcat tttttgtgcc
    26041 aacttttaat ttattgttat taaagagatt tttaagataa agatgaattt cgtcgccgtg
    26101 tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca acactggcac
    26161 aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg
    26221 ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac
    26281 tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg
    26341 ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca
    26401 agaggccgaa tgctggtgtg aaacatgcca acagtatgtg acgctactga cccagcgcgt
    26461 ccgccgctgt ccacagtgtc atggtgacat gctacagatt gtggcagacg acggtttaca
    26521 gattcggcgg atagaaatag accaggagtg agcgatgtgt acaacatgcg gttgcggtga
    26581 aggcaacctg tatatcgagg gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc
    26641 atttgccccg gcggcacgcc cgaagatgaa aatcaccggc attaaagcgc ctgaatttac
    26701 ccccagccag actgaagaag gcgacctgca ttacggtcat ggcgaagcgg gcactcacgc
    26761 accgggcatg agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg acaaaaataa
    26821 ccgtctggct gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct
    26881 ggtttccagc cctggttccg gtaaaaccac cctgctgacg gaaaccctaa tgcgcctgaa
    26941 agacagcgtt ccgtgcgcgg ttattgaagg cgaccagcaa accgtgaacg atgccgcacg
    27001 cattcgcgct accggtacac cagcgattca ggtgaacacc ggtaaaggct gccatcttga
    27061 cgcacagatg attgccgacg ccgcaccgcg tctgccactg gacgataacg gtattctgtt
    27121 tatcgaaaac gttggcaacc tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa
    27181 agtggcggtg ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt
    27241 tgccgccgcc tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc tcaactttga
    27301 cgttgagaag tgcctcgcct gcgcccgcga agtcaatcca gaaattgaaa tcatccttat
    27361 ttccgccacc agcggtgaag ggatggacca gtggctgaac tggctggaga cacagcgatg
    27421 tgcataggcg ttcccggcca gatccgcacc attgacggca accaggcaaa agtcgacgtc
    27481 tgcggcattc agcgcgatgt agatttaacg ttagtcggca gctgcgatga aaacggtcag
    27541 ccgcgcgtgg gccagtgggt actggtacac gttggctttg ccatgagcgt aattaatgaa
    27601 gccgaagcac gcgacactct cgacgcctta caaaacatgt ttgacgttga gccggatgtc
    27661 ggcgcgctgt tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg
    27721 aacaggtgat gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg
    27781 ccgaacgccc tctgcggatt atggaagtgt gcggtggtca tacccacgct atttttaaat
    27841 tcggcctcga ccagttactg cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg
    27901 tgtgcgtact gccgatgggt agaatcgaca cctgcgtgga gattgccagc catccggaag
    27961 tcatcttctg tacctttggc gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc
    28021 aggcaaaagc acgcggtgcc gatgtgcgca tcgtttactc gccgatggat gcgttgaaac
    28081 tggcgcagga gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca
    28141 tgccgaccac cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact
    28201 tcttctgcca gcacattacg cttatcccga cgctgcgcag tttgctggaa cagccggata
    28261 acggtatcga cgcgttcctc gcgccgggcc acgttagtat ggttatcggc actgatgcct
    28321 ataattttat cgccagcgat tttcatcgtc cgctggtggt cgctggtttc gaaccccttg
    28381 atctactgca aggcgtggtc atgctggtgg agcagaaaat agcggcccac agcaaggtag
    28441 agaatcagta tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa caggcgattg
    28501 ccgatgtgtt ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt
    28561 ctggcgtgca cctgacgccg gattatcaac gattcgatgc cgaagcacat ttccgcccgg
    28621 caccgcagca ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat
    28681 gtaagccgca tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc gcatttggtg
    28741 cactgatggt ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga
    28801 gtgaagcgtg aataatatcc aactcgccca cggcagcggc ggccaggcga tgcagcaatt
    28861 aatcaacagc ctgtttatag aagcctttgc caacccgtgg ctggcagagc aggaagacca
    28921 ggcacgtctt gatctggcgc agctggtagc agaaggcgat cgtctggcgt tctccaccga
    28981 cagttacgtc attgacccgc tgttctttcc tggcggtaat atcggcaagc tggcgatttg
    29041 cggcaccgcg aatgacgttg cggtcagtgg cgctattccg cgctatctct cctgtggctt
    29101 tatcctcgaa gaaggattgc cgatggagac actgaaagcc gtagtgacca gcatggcaga
    29161 aaccgcccgc acggcaggca ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg
    29221 cgcggcagat aaactgttta tcaacaccgc tggcatgggc gcaattccgg cgaatattca
    29281 ctggggcgca cagacgctaa ccgcaggcga tgtattgctg gttagcggta cactcggcga
    29341 ccacggggcg actatcctta acctgcgtga gcagctgggg ctggatggcg aactggtcag
    29401 cgactgcgcg gtgctgacgc cgcttattca gacgctgcgt aacattcccg gcgtgaaagc
    29461 gctgcgtgat gccacccgtg gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg
    29521 cggttgcggt attgaactca ctgaagcggc actgccggtt aaacctgccg tgcgcggcgt
    29581 ttgcgaactg ctgggactgg acgcccttaa ctttgccaat gaaggcaaac tggtgatcgc
    29641 cgttgaacgc aacgcggcag agcaagtgct ggcagcgtta cattcccatc cactggggaa
    29701 agacgcggcg ctgattggtg aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta
    29761 tggcgtgaaa cgaaccctcg atttaccaca cgccgaaccg cttccgcgta tatgctaata
    29821 aaattctaaa tctcctatag ttagtcaatg accttttgca ccgctttgcg gtgctttcct
    29881 ggaagaacaa aatgtcatat acaccgatga gtgatctcgg acagcaaggg ttgttcgaca
    29941 tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac
    30001 tggtaaagcg ttctgcgctc gccgacaatg cggctattgt gttgtggcaa gcgcagactc
    30061 aacgtgcgtc ttattacgcg tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa
    30121 ctgttctggc acacggtccg gtacgcagca ttttgtcgcg ccctgatacg ctgcattgca
    30181 gttacgaaga attttgtgaa acctggccgc agctggccgc aggtgggcta taccccaaat
    30241 ttggtcacta ttgcctgatg ccactggcgg cggaagggca tatttttggt ggctgtgaat
    30301 ttattcgtta tgacgatcgt ccctggagcg aaaaagagtt caatcgtctg caaacattta
    30361 cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgttaac aatgtcgact
    30421 atgagttgtt atgccgggaa cgcgataact tccgcatcct ggtcgccatc accaacgcgg
    30481 tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa atccattact
    30541 atttcgacat tgacgatatc agcattgtct tacgcagcca ccgtaaaaac aaactcaaca
    30601 tctactccac tcactatctt gataaacaac atcccgccca cgaacagagc gaagtcgatg
    30661 aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg atcaatctcc
    30721 acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga
    30781 ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc gtgctgaaac
    30841 tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttgctg cgccagattg
    30901 ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc catcgtctga
    30961 aagaacggct ggttgatgaa aacctcgccc tgaccgagca gctcaacaat gttgatagtg
    31021 aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa
    31081 tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggcacg ggtaaagagc
    31141 tgattgcccg cgcgatccat aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga
    31201 actgcgcggc gatgcctgcc ggattactgg aaagcgatct gtttggtcat gagcgtgggg
    31261 cttttaccgg tgccagcgcc cagcgcattg gtcgttttga actggcggat aaaagctccc
    31321 tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg ctgcgtgtat
    31381 tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg gacgtacgtt
    31441 taattgccgc gactaaccgc gatatgaaaa aaatggtcgc cgaccgagag ttccgtagcg
    31501 atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc gagcgtccgg
    31561 aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca
    31621 atatcgacag cattcctgcc gagacgctgc gcaccttgag caacatggag tggccgggta
    31681 acgtacgcga actggaaaac gtcattgagc gcgcagtatt gctaacacgc ggcaacgtgc
    31741 tgcagctgtc attgccagat attgctttac cggagcctga aacgccgcct gccgcaacgg
    31801 ttgtcgccca ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg aaagaaacca
    31861 acggcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc
    31921 tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa ctgcaaattg
    31981 ccggacagat ctgcctgtcc ggcatactat tcacgaggtt ttttcggacg atatttttcc
    32041 ggcagttctg gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga
    32101 cgccacagca ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca
    32161 ggctggtaac agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat
    32221 ttatccagcc gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac
    32281 gcctgcgggc atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga
    32341 gagatacgtt tgccggacat gctgcctcca cctcattaag atgtatttat attacatctt
    32401 aatcttaaag ggcactatga ctctaaagaa gaaaggttcg ccaaccgacg caattttgcg
    32461 tacttgcttc ataagcatca cgcaaaagct gcaaaacagc atctttcccg gaaccagcat
    32521 caagaactca ccgttcgctt cttcccctga aatgattaac tccggtatca tatgcgcctt
    32581 atgtgattac aacgaaaata aaaaccatca caccccattt aatatcaggg aaccggacat
    32641 aaccccatga gtgcaataga aaatttcgac gcccatacgc ccatgatgca gcagtatctc
    32701 aggctgaaag cccagcatcc cgagatcctg ctgttttacc ggatgggtga tttttatgaa
    32761 ctgttttatg acgacgcaaa acgcgcgtcg caactgctgg atatttcact gaccaaacgc
    32821 ggtgcttcgg cgggagagcc gatcccgatg gcggggattc cctaccatgc ggtggaaaac
    32881 tatctcgcca aactggtgaa tcagggagag tccgttgcca tctgcgaaca aattggcgat
    32941 ccggcgacca gcaaaggtcc ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc
    33001 atcagcgatg aagccctgtt acaggagcgt caggacaacc tgctggcggc tatctggcag
    33061 gacagcaaag gtttcggcta cgcgacgctg gatatcagct ccgggcgttt tcgcctgagc
    33121 gaaccggccg accgcgaaac gatggcggca gagctgcaac gcactaatcc ggcggaactg
    33181 ctgtatgcag aagatttcgc cgagatgtcg ctgattgaag gccgtcgcgg cctgcgccgt
    33241 cgcccgctgt gggagtttga aatcgacacc gcgcgccagc agttgaatct gcaatttggc
    33301 acccgcgatc tggtcggttt tggcgtcgag aacgcgccgc gcggactttg tgctgccggt
    33361 tgtctgttgc agtatgcgaa agatacccaa cgcacgaccc tgccgcatat tcgttctatc
    33421 accatggaac gtgagcagga cagcatcatt atggatgccg cgacacgtcg taacctggaa
    33481 attactcaga acctggcggg cggtgcggaa aatacgctgg cttctgtgct cgactgcacc
    33541 gtcacgccga tgggtagtcg tatgctgaaa cgctggctgc atatgccagt gcgcgatacc
    33601 cgcgtgttgc ttgagcgcca gcaaactatt ggcgcattgc aggatttcac cgccgagttg
    33661 cagccggtac tgcgtcaggt cggcgacctg gaacgtattc tggcacgtct ggctttacga
    33721 actgctcgcc cacgcgatct ggcccgtatg cgccacgctt tccagcaact gccggagctg
    33781 cgtgcgcagt tagaaactgt cgatagtgca ccggtacagg cgctacgtga gaagatgggc
    33841 gagtttgccg agctgcgcga tctgctggag cgagcaatca tcgacacacc gccggtgctg
    33901 gtacgcgacg gtggtgttat cgcatcgggc tataacgaag agctggatga gtggcgcgcg
    33961 ctggctgacg gcgcgaccga ttatctggag cgtctggaag tccgcgagcg tgaacgtacc
    34021 ggcctggaca cgctgaaagt tggctttaat gcggtgcacg gctactacat tcaaatcagc
    34081 cgtgggcaaa gccatctggc acccatcaac tacatgcgtc gccagacgct gaaaaacgcc
    34141 gagcgctaca tcattccaga gctaaaagag tacgaagata aagttctcac ctcaaaaggc
    34201 aaagcactgg cactggaaaa acagctttat gaagagctgt tcgacctgct gttgccgcat
    34261 ctggaagcgt tgcaacagag cgcgagcgcg ctggcggaac tcgacgtgct ggttaacctg
    34321 gcggaacggg cctataccct gaactacacc tgcccgacct tcattgataa accgggcatt
    34381 cgcattaccg aaggtcgcca tccggtagtt gaacaagtac tgaatgagcc atttatcgct
    34441 aacccgctga atctgtcgcc gcagcgccgt atgttgatta ttaccggccc gaacatgggc
    34501 ggtaaaagta cctatatgcg ccagaccgca ctgattgcgc tgatggccta catcggcagc
    34561 tatgtaccgg cacaaaaagt cgagattgga cctatcgatc gcatctttac ccgcgtaggc
    34621 gcggcagatg acctggcttc cgggcgctca acctttatgg tggagatgac tgaaaccgcc
    34681 aatattttac ataacgccac cgaatacagt ctggtgttaa tggatgagat cgggcgtgga
    34741 acgtccacct acgatggtct gtcgctggcg tgggcgtgcg cagaaaatct ggcgaataag
    34801 attaaggcat tgacgctgtt tgccacccac tatttcgagc tgacccagct accggagaaa
    34861 atggaaggcg tcgccaacgt gcatctcgat gcgctggagc acggagacac cattgccttt
    34921 atgcatagcg tgcaggatgg cgcggcgagc aaaagctacg gcctggcggt tgcagctctg
    34981 gcaggcgtgc caaaagaggt gattaagcgc gcacggcaaa aactgcgtga gctggaaagc
    35041 atttcgccga acgccgccgc tacgcaagtg gatggtacgc aaatgtcttt gctgtcagtg
    35101 ccagaagaaa cttcgcctgc ggtcgaagca ctggaaaatc tcgacccgga ttcactgact
    35161 ccgcgtcagg cgctggagtg gatttatcgc ttgaagagtc tggtgtaata acaattcccg
    35221 atagtctttt gctatcggga atattaacga caactgacga atcaaataaa aataccctgt
    35281 ataataggaa agcttatttt acagggtaaa accatgccat ctacacgcta tcaaaaaatc
    35341 aatacccatc actatcgcca tatatgggtc gttggtgata ttcatggtga atatcagtta
    35401 ttacaatccc gcttacatca actctctttt ttccccgaaa ccgacttact tatttctgtc
    35461 ggcgataata ttgatcgtgg gccggagagt cttgacgtcc tgcgcctgct aaaccagccc
    35521 tggtttatct ccgttaaagg caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc
    35581 gatggcaata tgtggcttgc cagcggtggt gactggtttt tcgatttaaa tgattcagag
    35641 caaaaagaag ctacagatct gttgctgaaa ttccatcacc ttccacatat tattgaaatc
    35701 actaacgaca acataaaata tgtcatcgca catgcagatt atccggggag tgaatatctc
    35761 tttggtaaag aaatagcgga gagcgaatta ctctggcctg ttgatcgtgt gcagaaatcg
    35821 cttaatggcg agttacaaca aataaacggc gctgattatt ttatatttgg acatatgatg
    35881 tttgataaca ttcagacgtt cgctaaccag atttatattg ataccggcac gccgaaaagc
    35941 gggcggctgt cattttataa aataaagtag tctcatgctt cttctgtgaa gcatgagtaa
    36001 ccaaatgtta ttgcaggcca ttattcattt ttcgctacca gcaaagagag atcctgcttc
    36061 gccagcgcgc gactggcact ctccggcaaa ccgtcgtctg taataatctg atcaaactcg
    36121 cttaatggta acgccagcca tgtcgccacc tgaccatatt tcgtcgcatc acagaccaaa
    36181 actcgctggc ggctggcact ggcaatcgcc cgtttcaccg tgactttatc ttccgctggc
    36241 gtagaaatcc cccgcacact ccatgacgat gcagaaataa aagcctgatc aatcatcagg
    36301 ctgcgcagca tggtcgcagc ggcttccccg acacaggaac ggttttcccg acacactgca
    36361 ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagttcgc aataacgaaa
    36421 tcgtttgtga ccacagtcag tgactccatg tgaatcagat gctgtgctat cgctaacgtg
    36481 gtcgttcccg catccagata gatacaactt cccggctgaa caagacttgc cgccagcttg
    36541 ccaatagccg ctttttgcgt cattgccagc gcagttttta cctgatgaga aggttcatgc
    36601 gccacgcgtc ccggagactg gacgcctccg gacaccagca caacggctcc ctgctgctcc
    36661 agtttttgta aatcccgacg aatggtcata tgtgacacat tcattctgtc cgttagttca
    36721 gcaatactga caatgccttt ttcagctacc atctcaagga tgatttggcg acgctctacg
    36781 ggtatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt gcctgcgaaa
    36841 cgtgcgcggc gcaactaacg cttagttcac ataaaataac acacaatgtt aatttatgtg
    36901 aataagctca ccatatcgtt atcttccagc gcttatattc acaatatcaa acaaaatatc
    36961 acttaaatta acaaggaggg cagatgaaaa cgggatctga gtttcatgtc ggtatcgttg
    37021 gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt ctttctacct
    37081 ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt gcttgcgggg
    37141 tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg ctggtggtca
    37201 atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa catctgaaac
    37261 ccggtacggc agtaatggtt tcttccacta tcgctagtgc tgatgcgcaa gaaattgcta
    37321 ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt ggtgcagtaa
    37381 aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc tttgaacgac
    37441 tggcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca gaaccgggac
    37501 taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt gctgccggag
    37561 ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg
    37621 tgaccaatgc cgccggaaat tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg
    37681 gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt ctggttgccg
    37741 atacagccaa agccctgcac ttcccgctgc cattggcctc aacagcattg aatatgttca
    37801 ccagcgccag taacgcaggt tacgggaaag aagacgatag cgcagttatc aagattttct
    37861 ctggcatcac tctaccggga gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt
    37921 taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa cggtacaaat
    37981 taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga tcagcctgaa
    38041 aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc tgagctggtt
    38101 gcaacagcaa ggttgcaaac agatctattt caaatactgc tctactttcg acagtacggc
    38161 gaaaggtaat atcggcccgg ttaccgatgc attaatggat gctctcgaca cgccgtttac
    38221 ggtcttctct ccggccctgc cggtcaacgg acgtacggtt tatcaggggt atttgttcgt
    38281 aatgaatcaa ctgctggccg aatccgggat gcgccatcac ccggtaaatc ccatgaccga
    38341 cagctatctt ccccgtctgg ttgaagcgca atccgcaggg cgctgtggcg tcgtttcggc
    38401 acatgttttc gaacaaggtg tggatgccgt tcgtcaggag ctggctcgct tacagcaaga
    38461 gggctatcgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa ttcagggaga
    38521 agccttgcgc gatgccccac tggtaacggg cggttctggt ctggcgattg gcctggcccg
    38581 gcagtggacg caagaaaacg gtaaccaggc tcgcaaagca gggcatccgc tcgctgggcg
    38641 cggcgtagtg ctctccggtt catgctctca aatgaccaac cgccaggtag cacattaccg
    38701 tcaaattgca ccagcccgtg aagttgatgt ggcacgctgc ctctcaactg aaactctggc
    38761 tacttatgca cacgaactgg cagagtgggt tctgggccag gaaagtgtac ttgctccact
    38821 ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat acggtgcaca
    38881 aaaagccagt caggcagtag aaacactgtt ttctcaacta gcggcgcggt tagcggcgga
    38941 aggcgtggca cgctttattg tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct
    39001 gggcataaaa gggtttcata ttggcccaac catttccccc ggcgtgccgt gggtaaacgc
    39061 actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg aagccttttt
    39121 ttcacgagcc caaagagagt ttttatcatg agcgatttcg caaaagtaga gcagtctttg
    39181 cgagaggaga tgacgcggat tgccagttca ttctttcagc gcggctatgc aaccggttcg
    39241 gctggcaatc tgtcgctgct tttacctgac gggaatttac tggcgacacc gacaggttca
    39301 tgcctgggca atctcgatcc gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta
    39361 agtggtgaca aaccctcgaa agaggtgctc tttcatctgg cgctgtatcg caacaatccg
    39421 cgctgtaaag cggtggtgca tttgcacagc acatggtcga cggcgctttc ctgcctgcaa
    39481 gggctggaca gcagcaacgt tattcgtccg ttcacaccat acgtggtgat gcggatggga
    39541 aatgtcccgc tggtgcctta ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg
    39601 gaactggcag cagacaatca ggctttttta ctggcaaatc atggcccagt ggtttgcggt
    39661 gaaagcctgc aagaagccgc caacaatatg gaagagctgg aggaaacggc aaagctgatt
    39721 tttattctcg gtgaccgccc gatccgttat ctgaccgcag gtgaaattgc ggaattaagg
    39781 agttaatgca atgcctcgtt ttgcagctaa tttatccatg atgttcaccg aagtgccttt
    39841 tattgaacgc ttcgccgcag cgcgaaaagc cgggttcgat gctgtggaat ttctgtttcc
    39901 ctatgactac tccaccctgc aaatccaaaa gcaactggag caaaaccatc tgacactggc
    39961 gctgttcaat accgctcccg gagatattaa tgcgggggaa tggggattat ccgcccttcc
    40021 cggacgtgag cacgaagcac acgcagacat cgacctggca ctggaatatg cattggcgct
    40081 caattgtgaa caagtccatg tgatggcagg cgtcgtgccc gctggcgaag acgctgaacg
    40141 gtaccgggca gtatttatcg ataatctccg ctacgctgcc gaccgctttg cgccacacgg
    40201 caagcgaata ttagttgaag cactcagccc cggcgttaag cctcattatc tcttctccag
    40261 ccagtatcag gcactggcta ttgttgaaga ggttgcgcga gataacgtgt ttattcaact
    40321 agatactttt catgcgcaaa aagtagatgg caacctgacg catttaatcc gcgactacgc
    40381 cgggaaatat gcgcacgtac aaattgccgg actacctgat cgacatgaac cggacgatgg
    40441 agaaatcaac tatccgtggc tgttccgcct gttcgatgag gtgggatatc agggctggat
    40501 cggttgtgaa tataaacctc gtggcctcac cgaagaaggg cttggctggt ttgacgcctg
    40561 gcgctaattc gttaaatccc taattacaac gtacccatac atccccccat ctacggatgg
    40621 ggtaggttaa ctataacttt cagacagggt ttcccatgtc cacaattaca ttgttatgca
    40681 ttgcgttagc tggcgtaatc atgctgttgc tgctggtcat caaggcaaag gtacaaccat
    40741 tcgttgctct gctcctcgtc agcctgttag tcgcacttgc ggcgggtata ccggcgggcg
    40801 aagtgggtaa agtgatgatc gccgggatgg gcggtgtgct tggttccgtc actattatta
    40861 ttggtctggg cgctatgctg gggcgtatga tcgaacactc tggtggtgca gagtcactgg
    40921 ctaattattt cagtcgcaag ttaggtgaca aacgaactat cgctgcgctg actctggcag
    40981 cgttcttcct cggtattccc gtcttctttg atgtcggctt tattattctt gcgccaatca
    41041 tttacggttt tgccaaggtt gccaaaatat cgccactcaa atttggcctg cctgtcgctg
    41101 ggatcatgct aactgtacat gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg
    41161 ggttactcca cgcagacatc ggctggctaa ccatcatcgg tattgcgatt tctattcccg
    41221 tagggattgt tggctacttt gcagcgaaaa taatcaataa gcaccaatat gcgatgtcgg
    41281 tggaagtact ggaacagatg caactggctc cggccagtga ggaaggcgcg acaaaattaa
    41341 gcgataaaat aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg
    41401 cgattatcat ggcgggtacg gtttccgcaa cactgatgcc gccttcgcat cccctgcttg
    41461 gtacgctaca gctgatcggc tcaccaatgg tagctctaat gattgcgctg gtgctggcat
    41521 tctggttatt ggctttacgt cgcggctgga gcttacaaca caccagcgac attatgggct
    41581 cagcccttcc cactgcggcg gtagtgattt tggttactgg tgctggaggg gtatttggca
    41641 aagtgctggt ggaatcgggc gtcggcaaag cccttgccaa catgctacaa atgattgacc
    41701 tgcctctgtt accagccgca tttattattt cactggcgct gcgtgcatcg caggggtcag
    41761 caaccgtagc aatcctgaca accggcgggt tactctcaga agcggtgatg ggattgaatc
    41821 cgattcagtg cgtattggtg acgctggcag cctgctttgg tgggcttggc gcgtcacata
    41881 ttaatgactc agggttctgg attgtgacca aatatctggg gttgtcggta gcagacggtc
    41941 tgaaaacctg gacagtgtta acgaccattc tcggttttac cggattctta attacctggt
    42001 gcgtatgggc ggtaatttga ccttaataaa aaggtccgat gggcatcgga cctttttatt
    42061 tagcgcttac cttccgccag cagcggcggg atgctcggca ccattggcgc atcatcaata
    42121 tctttctgcg tcatgcggaa cgcttccggg taatgttcgc gactggtgcg gcgtagcggt
    42181 tcggtatcgc gccaggtata aaggcaatgc tggcactgat ataccgtcca gacatctttc
    42241 accggcgatt tcaccatcac ttcaatctgt tcatcggcac aacgtggaca aatcatcttg
    42301 ttctccttat ttacgtgcag ccagcatagt ggtcagtttt tcagcccagg ctttggtttc
    42361 cggcaaatcc accaccggct ggctgtagtg accacggttg tccggggcga caggcgtggt
    42421 agcgtcgata atcagcttgt cggtgatccc cgccgggctt gagccagggt cgagttccag
    42481 cacggacata ttcggcaact gcaccaaatc ccctgccggg tttactttcg aggagagcgc
    42541 ccacatcacc tgcggcaggt tgaacgggtc aacgtcttca tcgaccataa tcaccatctt
    42601 tacgtagccc agaccgtgcg gcgtggtcat cgcacgcagg cccaccgcgc gggcaaagcc
    42661 gccgtagcgt tttttggtgg agataatcgc cagcaggccg tgggtgtaca tggcatttac
    42721 cgcctgcact tccgggaact cggctttcag ttgttgatac agcggtacgc aggtggctgg
    42781 ccccatcagg tagtcgattt cggtccacgg cataccgaga tagagtgatt cgaaaatcgg
    42841 tttgctgcga taagagactt tatcgatgcg caccacggtc atgttgcgcc caccggagta
    42901 gtgcccggta aactcaccga acggcccttc gatttcgcgt ttacggcttt cgataacccc
    42961 ttcgaggatc acttctgaac cccacggcac atcaaaaccg gtcagcgggg cggtggcgat
    43021 cgggtacggg ctttcacgca gcgcgcctgc catttcatac tcagactgat cgtatttcag
    43081 cggcgtggcc cccatcagcg tgatgatcgg atcgttaccg agcgtgatcg caatcggcag
    43141 atcttcaccg cgctcttctg ctttatgcag atgcagggcg atatcgtgca tcggcaccgg
    43201 ttgcaggccg agcttacgct tgcccttcac ttccatgcgg tagatgccga cgttctgctt
    43261 gccgaagtta tctgggtcga gcggatcgcg ggaaaccacg caggctttgt cgagatagaa
    43321 accgccatcg ccatcgttta aacgaaacag cggcaggata tcgaacaggt taatctcttc
    43381 gccatcgacg gtgttctgcg cccaggctgg attggcgcgg cgctccgggg caatcgggaa
    43441 gttatcccag cggcggataa actcatcaat ctgctttttg accggggtgt ttggtggcag
    43501 gccaagggaa atcgcgtggt tctgccagga accgatggta ttcatcgcca cgcgggcatc
    43561 ggtaaagccg cgaatattat caaaccacag cgcgggcgcg ccgtcgccga tacgcccggt
    43621 ggcgttagct gctgctgcca gatccggctc agcgttcacc tcttcgctga ttttcagtaa
    43681 ctggccgtgg tcatcaagcg cctgtaaaaa gctgcgtaaa tcatcaaatg ccattattca
    43741 ttctcctgag aaaaattccg ggcctgcggc aatccttgcc agcgcctggc atggggatgt
    43801 tcgaggccaa attgatccag cacgcgggct accacatggt ggacaatgtc atctaccgtt
    43861 tcgggatggt tataaaaggc aggcatcggc ggcaccatcg ccacgcccat gcgtgaaagt
    43921 gcgagcatat tttcgagatg gatggtgcta agcggcattt cacgcggcac cagcaccagt
    43981 ttgcggcctt ctttgagcac gacgtccgcc gcgcgcccta ccaggccatc ggcgtaacca
    44041 gcgcggatac cggcgagcgt tttcatactg cacggaataa cgatcatgcc gtcggtacga
    44101 aaagaaccgg atgagatgat cgccgcctga tccgccgggt tatggctgaa gtctgcgagg
    44161 gcagcaacat cgcgagcgct gtaaggcgtt tccagttcaa tggtggtttt cgcccacttc
    44221 gacatcacca gatgagtctc gacattcggc atatcccgca gcgcttgcag taatgccaca
    44281 ccaagaggcg caccggtagc ccctgtcatc ccgacgatca gtttcattgc ttatcctctt
    44341 aattgttcgt atacgaacgt tattattaac atactccttc atttccacag cggcaaggat
    44401 tgaaaagtaa caacatcttc ttcgtcacta aaaaaccgct atgatagcga cagagaatta
    44461 gcccggagtt tacatggcgt tacgaaataa agcgttccat cagttacgac agctttttca
    44521 gcagcacacg gctcgctggc agcacgagtt acctgacctg accaaaccac agtacgcggt
    44581 gatgcgcgcc attgctgata aacctggtat cgaacaggtg gcactgatag aagcagctgt
    44641 cagcaccaaa gcaacgttag cagaaatgtt ggcaagaatg gaaaatcgtg gcttagtcag
    44701 acgtgaacat gaccccgctg ataagcggcg acgctttgtc tggttgactg ccgaaggcga
    44761 aaagatactt gctgcggcga tacccattgg tgacagcgtg gatgaggaat ttttggggcg
    44821 tttgagtgga gaagagcagg agctgtttat gcagctggtg cgcaagatga tgaacgcata
    44881 gggggcaaat ttccggatgc gacgctatcg cgtcttatcc agactacaaa tacaccgact
    44941 caccaaccga aagaaaaaag gccagcctcg cttgagactg gcctttctga cagatgctta
    45001 cttactcgcg gaacagcgct tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac
    45061 ggcgcaggcc ttcaacctga atctggcgaa cacgttcacg ggtgaggcca atttcacgac
    45121 ctacatcttc cagtgttgcc gcttcgtacc ccagcaaacc gaatcgacgt gccagcactt
    45181 cacgctgttt ggcgttcagc tcgaacagcc atttgacgat gctctgcttc atatcgtcat
    45241 cttgcgtggt atcttccgga ccgttctctt tttcatcggc caggatgtcc agcaacgctt
    45301 tttcggaatc accacccagc ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca
    45361 tacggctgac gtcatcaact ggcttatcca gttgctctgc gatctcttcc gcacttggtt
    45421 catggtccag cttatgggac aactcacgtg cggttcgcag gtaaacgttc agctccttta
    45481 cgatgtgaat cggcaaacga atagtacggg tttggttcat aatcgcccgt tcaatcgtct
    45541 ggcgaatcca ccaggttgcg tatgttgaga agcggaaacc acgttccggg tcaaacttct
    45601 ctaccgcgcg gatcagcccc aggttgccct cttcgataag gtccagcaac gccagaccac
    45661 gattgccata acggcgggca atttttacca ccagacgcaa gttactctcg atcatccggc
    45721 ggcgagaggc gacatctcca cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg
    45781 ttaacagtgg tgaataacca atctcaccaa ggtaaagctg agtcgcgtcc aacacacgct
    45841 gtgtggctcc ctgcgataac agttcctctt cggccaaatc gttatcactg ggttcctctt
    45901 ctactaaggc cttttcgtca aaaacctcaa ctccgttctc atcaaattcc gcatcttcat
    45961 ttaaatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc gtgatccctt
    46021 gacggaacat tcaagcaaaa gcctggttcc gccgatttat cgctgcggca aataacgcag
    46081 cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc
    46141 ggtgctaccc atggttgcta ttttttgccc cgccttcact tcttgttgtt cccggaccag
    46201 cattgtgtcg ttatgggcgt aggcactcag gtaatcatca ttatgtttga tgataatcag
    46261 attaccgtag ccgcgcagcg cgttaccggc ataaacaacg cggccatctg cggtcgcgat
    46321 aattgcctgt cctttgctgc ctgcgatatc aatccccttg ttgcccccct cagaagcgcc
    46381 aaaggtttcg atcactttgc cctcagtcgg ccagcgccag gtggagatag gcgtactggt
    46441 tgatgtactg ctgacagttg gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt
    46501 gaccgtggtc gcagctggct tgttgttcgg caacattttg ttagcactct gttcacccga
    46561 agattcagaa tacgtaattg tcggttgcga cgcaacagca acggtggaat tttgtgcagg
    46621 cttgatcaca actccttgct ctgctgcgtc ggcctgggta atggcatttc cgccagtgat
    46681 tggcgtaccg gaagcattac ccacctgcaa ggtctgacca acgttcagcg cgtatggtgc
    46741 ctgaatattg ttgcgctgag caaggtcacg gaaatcgttg ccagtaatcc aggcgatata
    46801 gaaaagtgtg tcgccttttt tcacggtata ggtactgccg ctataactgc ctttcggaat
    46861 gttcccatac tgacggttat agacgatgcg tccgttttcc atctgtaccg gctgctgagc
    46921 tactggctgc actggctgga tttgcggttg ttgagtagcc tgaatttgtg gctgctgtac
    46981 cggctgaatt tgcggttgct gggctgtaga cgtcgtcccc attttcggcg gcggcgtaat
    47041 caacatacca gaattggtat ttgcaggcgc attgccatta acggagctga ccggtgccgg
    47101 tggatttgaa gtgtcagaac agcctgccag ccatagcgaa accagtgaca aagccgcaat
    47161 gcggcgaacg gtgaattttg ggcttcccgc gctcatttat cccccaggaa aaattggtta
    47221 ataaccagtg acataattac cgtgcaaggc accctactga acactggaaa agatgttcac
    47281 gatacgctga cctgcggcaa aataaccagg aaaaatccag gtatttcctc acgttttaag
    47341 ccagctcacc cttcactaaa gggacaaagc gcacggcctc cacggtatcg ataataaatt
    47401 cgcctccccg acgacgcacc cgtttcaaat actggtgctc ctcccctacg ggtaagacga
    47461 gaatcccgcc ttcgtccagc tgcgtcatta gcgcagttgg aatttccggc ggtgccgccg
    47521 taacaatgat agcgtcaaac ggcgcgcgtg cctgccaacc ttgccatcca tcgccatgac
    47581 gggttgaaac attatgtaaa tcaagatttt tcaggcggcg acgcgcctgc cactgcaaac
    47641 ctttaatccg ttcgaccgag caaacatgct ggacaagatg cgccaggatt gccgtttgat
    47701 atcccgaacc ggtgccaatt tccagcaccc gcgactgcgg cgtcagctcg agtaattcgg
    47761 tcattcgcgc caccatatac ggctgcgaaa ttgtctgccc ctgacctatc ggcaaggcga
    47821 tattgtccca ggctttttgt tcaaacgctt catcaacgaa tttttcacgc ggcacggcag
    47881 caagtgcatt cagcacctgc tcatcctgaa taccttgcgc acgtaattga tccagaagtg
    47941 cttgtacgcg tctgcttacc attgcgtgcc aactcccacg ctgtttaacc agtctgaaac
    48001 cacatcttgc gcgctatgcg cagttaaatc cacatgcagc ggcgtgatgg agacatagcc
    48061 ctcatctacc gcagcaaaat cggtccccgg accggcatca catttaccgc ccggcgggcc
    48121 aatccagtac agcgtattgc cgcgcggatc ttgctgcggg atcacctgat ctgccggatg
    48181 tcgtgtaccg cagcgcgtca cgcgaatacc tttgatttga tccaagggta aatccggaac
    48241 gttaatatta agaatacgcc cggtgcgcaa cggctcttta cacagtgcgc gcaaaattga
    48301 acaggttacc gccgcggcag tgtcgtaatg tttatgcccg tcaagcgaga cggcaagcgc
    48361 cggaaaacct aaatgacggc cttccatcgc ggcggctacc gtaccggaat aaataacatc
    48421 atcccccaga ttcggcccgg cgttaattcc ggacacaaca atgtccgggc gcggacgcat
    48481 cagagcattc acgccaagat agacgcaatc ggtcggggtt cccatttgca cagcaatatc
    48541 accattttca aaggtaaacg tgcgcaggga ggattccagt gtcagagaat ttgaagcgcc
    48601 gctgcggtta cgatcggggg cgaccacctg aacgtcagca aactcacgca aggctttcgc
    48661 cagcgtttgt ataccgggtg catgtacccc gtcatcatta ctcagcaata tgcgcataat
    48721 cacctgttgt gttgataagt tccctgacaa cgctggttgc aaaactaccc gccggaagcc
    48781 agaaacggat ctctacggtg acgtcatccc accaattcca gcttaattgt tgcggataca
    48841 gcagcatcgc tctgcgcgcg gcttcaactt tttcgcgcac cagtaaagct tgtaattcag
    48901 tttctgcggc gacagctgct tgttcgaatg ccagcgcttc acgctgagtt ccccattcgc
    48961 cactgcccgg caatgcggcg gttatcatca gctctttatc gttaacgcga cgctgtaatt
    49021 ccgccagttc ttcggtggtt gcgacaaacc agctaccacg tccggctaat tgtagcgcat
    49081 cgccgtcaac aacttgatta acgtctgctt ttttgaggcg ctcagcaaca atctgattaa
    49141 acaacgcact gcgggctgcc gacaaccaaa aactccgttt attgcgatcg cgcaccggag
    49201 tattggtttg cgcccagcgc agcgcgccct gcaagttgct accgccaatc ccaaaacgtt
    49261 gggcaccgaa gtagttcggt acacctttta cgcaaatatc gatcagacgt tgttcaacgt
    49321 catcgcgatt gctcacttcg cgcagaacca gggtaaaggc gttacctttc agcgccccta
    49381 aacgcagctt gcgcttgtgc cgcgcatact ccagcacctg gcagccttcc agttgaaagg
    49441 cgctcagatc gggcatttcc ttgcccggca cgcgagcaca taaccactgt tccgtaacag
    49501 catgtttatc tttttgccca gcgaagctga cttcacgggc atgaattttc aggaatttcg
    49561 ccagtgcatc cgccacaaaa cgggtattgc agccgttttt gaggattcta accagaatat
    49621 gctcaccttc accatcaggc tcaaagccca aatcttccac caccacaaag tcttccggat
    49681 tggctttcag cagcccggtg ccttgcggtt taccgtggag gtaagtgaga ttatcaaact
    49741 caatcatttt gttgccttaa tgagtagcgc caccgcttca caggcaatcc cttccccacg
    49801 tccggtaaat cccagttttt ccgtagtagt ggctttcacg ttaacatcat ccatatggca
    49861 gccgagatct tcggcaataa acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc
    49921 ctgagcgatg atagtgacat cgacgttgcc aagggtataa cccttcgcct gaatacgacg
    49981 ccaggcttcg cgtagcagct cgcggctatc ggcaccttta aatgccggat cggtatccgg
    50041 gaacagtttg ccgatatccc ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg
    50101 gagcgccacg tcgccatcag aatgcgccag caatcctttt tcgtaaggaa tgcgtacgcc
    50161 accaatgata attgggcctt caccgccaaa ggcgtgtacg tcaaaaccgt gtccaattcg
    50221 cattatgtat tctcctgatg gatggttcgg gtgaggtaaa actcggccag tgccaaatct
    50281 tccgggcgcg tgactttaat gttatccgcg cggccttcga ccaactgcgg atgaaatccg
    50341 caatattcca gcgccgaggc ttcgtcagta atagtcgcgc cttcatttag agcgcgcgtc
    50401 agacagtcat gtaacagctc acgagggaaa aattgcggcg tcagcgcgtg ccataagccg
    50461 ttgcgatcaa cggtatgagc aatggcattt ttgcccggtt cggcacgttt catcgtatcg
    50521 cgcactggtg cggctaggat ccctcccgtg cggctggttt cgctcaacgc caacaatcgc
    50581 gcgaggtcat cctgatgcag acaaggacga gcggcgtcat gcaccaatac ccactgcgcg
    50641 tcgccagcgg ctttcaaacc tgccagcacg gaatcggcac gctcatcacc gccatctaca
    50701 acggtgattt gcggatgatt cgccagagga agttgtgcaa aacggctatc gccaggactt
    50761 atggcaatga cgacacgttt cacccgggga tgcgccagca gcgcatgcac cgagtgttca
    50821 agaatggttt gattaccgat tgagagatat tgcttaggac attccgtttg cattcgacgg
    50881 ccaaatccgg ccgccggaac cacggcgcaa acatccaaat gagtggttgc catgttaatt
    50941 cccgggctga tttatcgatt gttttgcccc gcagactgtg cgcgcttcga cgcgtcaggc
    51001 accagacgat aaaaagtttc gcccggcctg gtcatgctga gttcattacg cgcacgctct
    51061 tcgagcgcct cctggccgcc attgagatcg tcaatttcgg caaaaagttg atcgtttcgc
    51121 gctttaagtt tcgcgtttgt agcttgctgt gccgccacat catcattgac gcgggtatag
    51181 tcatgtatac cgttcttacc gaaccacagc gaatactgta gccagaccag aatagccagc
    51241 aacagcagcg ttagtttacc catcctgccc cctgaaaaac ggcatcatca tcccatgcat
    51301 ccgaagacga ctctacatcc tctgttgggg ataccgcgac aacgcgggca aatgtaccac
    51361 atttgtccat tgttacgtat acccagggcg tgcagaacat aatctcatta ttagttacgg
    51421 tttgaattat gaacagagga gacaagaaag tacaaattag cccagtagcc acataaacag
    51481 tgcgccaaac ataatgccta ctgtcaccag ggtgaaaaca atactgtagc gtagctttcc
    51541 gtccatcaat gaatgcagcg caatccccac cactaccgcg acaggcatca gtgccagaaa
    51601 gaaaggccag gtgtagataa agaagaacag cgtgttagag ccataaatca acatcggcat
    51661 cgccagcgca aataaccagg agataaaacc gaccacggca ccaggcagtg accatgtggt
    51721 ttcttcatcc tcagtaaggc tgtcgttatt tgttagtgta atgttatggc tattacgcat
    51781 atttgatcct gttactttga cgaaccgggc atggaaaccc ggtggtgtct caggatctga
    51841 taatatcgtt ctgtctcaac agatctaata attgctgtac caaatttgtt actaattgtt
    51901 caccattgag atgaatttct gccgattcag gcgcttcgta aacggaatct attcccgtaa
    51961 agttgcgcag ttcaccggca cgcgctttct tatataagcc tttgggatcg cgggcttcgc
    52021 aaatcgccag cggcgtatcg acaaacactt cgataaagcg cccttctcct acgcgttcgc
    52081 gaaccatctg gcgttcggcg cggtgtggcg agataaatgc ggtcagcacc accagtccgg
    52141 cttcaaccat caaattcgcc acttcaccga cgcgacggat attctcttta cgatcggcat
    52201 cgctaaaacc gagatcgctg cataatccgt ggcgaacatt gtcgccatcc agcagatacg
    52261 tactgacgcc gagtttatgt aacgcctcct ccagcgcccc ggcgaccgtt gatttaccgg
    52321 acccagagag gccggtaaac cacagcacta caccacgatg accgtggtgt agctcgcgtt
    52381 gttgcacagt gaccggatgg ctatgccaga cgacgttttc gtcatgcagc gccattattt
    52441 atcccccagc aaatcgcgcg cgccccagtg cggaaagtgg cgacgaacca gggcattcaa
    52501 ttccagttcg aatgcactga attcagatga cgcaacggcg gcctcgtttt tcggctcatg
    52561 caccataccg gcaccaacag tcacgttgct caggcgatcg ataaaaatca gcccacccgt
    52621 caccggattt tgttgataac gatctaacac cagcggctcg tcaaaagtga gatccacgag
    52681 gccgatccca ttcagtggca ggttttcaac ttcacgctgg gtaaggttat taatatcaac
    52741 ctgatagcga atgccatcaa tacgcgcgcg cgtcttctta ccggcaattt tgatgtcgta
    52801 actctggccc ggggaaagcg gctgttccgc catccatacc acatccaccg acgcgctctg
    52861 caccgccggt aacgcttcgt ctgccgccag cagcagatcg ccacggctga tgtcgatctc
    52921 atccgtcagc accagggtga tcgcttctcc ggcaaaggct tcttcgcgat caccatcaaa
    52981 agtcacgatc cgcgcgacgt ttgattccac accagagggc agcactttga cacgttgccc
    53041 gacttccacg cgaccggatg ccagcgttcc ggcgtagcca cgaaaatcga gattcgggcg
    53101 attaacgtac tgcaccggga agcgcattgg ctgagcatcc accactcgct ggatctccac
    53161 ggtttccagc acttcgagca gtgtcggacc gctgtaccac ggcatacttt cactttgcga
    53221 agccacgttg tcgccttcca gcgcagagag cggcacaaag cgaatatcca gattgccagg
    53281 taactgttcg gcgaaagtca ggtagtcttc acgaatgcgg gtgaatgtct cttcactgta
    53341 atccaccaga tccattttgt tgatcgctac gaccagatgt ttgatcccca gcagcgtgga
    53401 gataaaactg tgacgacggg tttgatcgag cacgccttta cgggcatcga tcagtaagat
    53461 cgccagctca cacgttgagg cgccggtcgc catattgcgg gtatactgct cgtgcccggg
    53521 ggtgtcggcg ataataaatt tacgcttctc ggtagagaaa tagcggtagg ccacatcaat
    53581 agtgatgccc tgttcgcgct cagcttgcag gccgtccacc agcagagcca gatccagttt
    53641 ttcgccctgg gtgccgtgac gcttactgtc gttatgcagc gatgagagct gatcttcgta
    53701 gatttggcgg gtatcgtgca gcaggcgacc aatcagggta cttttgccgt catcgacgct
    53761 accacaggtc agaaaacgca gcaggctttt atgttgttgc gcaatcatcc aggcttcgac
    53821 gccgccttca ttagcgattt gttgtgcaag tgcggtgttc atcttaaaaa tacccctgac
    53881 gttttttcag ctccatagac cccgcctggt cgcggtcaat cacgcggccc tgacgttcac
    53941 tggtggtgga aaccagcatc tcttcgatga tctccggcag tgtttgtgca tttgactcca
    54001 ccgcaccggt cagcggccag cagcccagcg tacggaaacg caccatccgt tttttaatca
    54061 cttcgcccgg ttgcaggtcg atacggttgt catcaatcat catcaacata ccgtcgcgtt
    54121 ccagaaccgg acgttccgca gcgagataca gcggcacaat gtcgatattt tccagccaga
    54181 tgtattgcca gatatcctgc tcggtccagt tagagagcgg gaaaacgcgg atgctttcgc
    54241 ctttgttaat ctgcccgtta tagttgtgcc acagctccgg gcgctgattt ttcgggtccc
    54301 agcgatggaa gcgatcacgg aaagagtaga tacgctcttt agcacgggat ttctcttcgt
    54361 cgcggcgcgc accaccgaag gcggcatcaa aaccgtattt attcagcgcc tgcttcaggc
    54421 cttcggtctt cataatatcg gtatgtttcg cgctgccgtg cacgaatgga ttaatcccca
    54481 tcgccacgcc ttccgggttt ttatgcacca gcagctcgca gccgtaggct ttcgccgtac
    54541 gatcgcggaa ctcatacatc tcgcggaatt tccagccggt atcgacatgc agcaacggga
    54601 aaggcagcgt acctggataa aacgccttgc gcgccaaatg cagcatgacg ctggaatctt
    54661 taccgataga gtagagcatc accggatttg agaattctgc cgccacctcg cgaataatgt
    54721 ggatgctttc cgcctccagt tgccgcaggt gagtaagtcg tttttgatcc ataaccgttc
    54781 ctttgcaata ccgctatttt cttgccatca gatgtttcga ctatagggag cgtaagagaa
    54841 cgaatgaaat taccaattag aatgagtagt tccttaacgg aataacgatt tggcaaagct
    54901 aatatcaaaa agtgcttaag gcaccggatt tcgggcgttt aggaagattt gaaattgttt
    54961 tagcgcagcg gcagtttcat actatggcgg taaaaaaatt tgcatggtat ttaaggactc
    55021 actatgtttt ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt atgctttatt
    55081 ctccccgtac acgcctcgtc acctaaacct ggcgattttg ctaataccca ggcacgacat
    55141 attgctactt tctttccggg acgcatgacc ggaactccag cagaaatgtt atctgccgat
    55201 tatattcgcc aacagtttca gcaaatgggt tatcgcagtg atattcggac atttaatagt
    55261 cggtatattt ataccgcccg cgataatcgc aagaactggc ataacgtgac aggaagtacg
    55321 gtgattgccg ctcatgaagg caaagcgccg cagcagatca tcattatggc gcatctggat
    55381 acttacgccc cgctgagcga tgctgacgcc gatgccaatc tcggcgggct gacgttacaa
    55441 ggaatggatg ataacgccgc aggtttaggt gtcatgctgg aattggcaga acgcctgaaa
    55501 aatacgccta ccgagtatgg tattcgattt gtggcgacca gcggcgaaga ggaagggaaa
    55561 ttaggcgctg agaatttact caagcggatg agtgacaccg aaaagaaaaa tacgctggtg
    55621 gtgattaatc tcgataactt aattgttggc gataaattgt atttcaacag cggtgtaaaa
    55681 actcctgaag cagtaaggaa attaacgcgc gacagggcgc tggcaattgc gcgcagtcac
    55741 ggaatagccg caacgaccaa tccgggtttg aataaaaatt atccgaaagg cactgggtgt
    55801 tgtaatgacg cagaaatatt cgacaaagcg ggcattgctg tactttcggt ggaagcgact
    55861 aactggaatc ttgggaataa ggatggttat cagcaacgcg caaaaacagc cgcattccct
    55921 gcgggaaata gctggcatga cgtaagactg gataatcaac agcatattga taaggcactt
    55981 cctggaagaa tagaacgtcg ctgccgtgac gttatgcgga taatgctacc gttggtgaag
    56041 gagctggcga aggcgtcttg atgggtttga aaatgggagc tgggagttct accgcagggg
    56101 cggggaattc taagtgatat ccatcatcgc atccagtgcg cccggtttat ccccgctggc
    56161 gcggggaaca ccagattatc ttctttttca atccagccac cgacggttta tcccc
//
LOCUS       DAZFAH010000010        53283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFAH010000010 DAZFAH010000000
VERSION     DAZFAH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.010"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHJWB_000561"
     CDS             complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHJWB_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4312243.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            546..2807
                     /gene="katE"
                     /locus_tag="ACHJWB_000562"
     CDS             546..2807
                     /gene="katE"
                     /locus_tag="ACHJWB_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4312244.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3066..3824)
                     /gene="chbG"
                     /locus_tag="ACHJWB_000563"
     CDS             complement(3066..3824)
                     /gene="chbG"
                     /locus_tag="ACHJWB_000563"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4312245.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3837..5189)
                     /gene="celF"
                     /locus_tag="ACHJWB_000564"
                     /gene_synonym="chbF"
     CDS             complement(3837..5189)
                     /gene="celF"
                     /locus_tag="ACHJWB_000564"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4312246.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5294..6136)
                     /gene="chbR"
                     /locus_tag="ACHJWB_000565"
     CDS             complement(5294..6136)
                     /gene="chbR"
                     /locus_tag="ACHJWB_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4312247.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6144..6494)
                     /gene="chbA"
                     /locus_tag="ACHJWB_000566"
     CDS             complement(6144..6494)
                     /gene="chbA"
                     /locus_tag="ACHJWB_000566"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4312248.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMYQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6545..7903)
                     /gene="chbC"
                     /locus_tag="ACHJWB_000567"
     CDS             complement(6545..7903)
                     /gene="chbC"
                     /locus_tag="ACHJWB_000567"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4312249.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDTSTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGVNIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7988..8308)
                     /gene="chbB"
                     /locus_tag="ACHJWB_000568"
     CDS             complement(7988..8308)
                     /gene="chbB"
                     /locus_tag="ACHJWB_000568"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4312250.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8607..8945)
                     /gene="osmE"
                     /locus_tag="ACHJWB_000569"
     CDS             complement(8607..8945)
                     /gene="osmE"
                     /locus_tag="ACHJWB_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4312251.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQLVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9147..9974
                     /gene="nadE"
                     /locus_tag="ACHJWB_000570"
     CDS             9147..9974
                     /gene="nadE"
                     /locus_tag="ACHJWB_000570"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4312252.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10204..11091
                     /gene="cho"
                     /locus_tag="ACHJWB_000571"
     CDS             10204..11091
                     /gene="cho"
                     /locus_tag="ACHJWB_000571"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4312253.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11051..11626)
                     /gene="ves"
                     /locus_tag="ACHJWB_000572"
     CDS             complement(11051..11626)
                     /gene="ves"
                     /locus_tag="ACHJWB_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4312254.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11829..12314)
                     /gene="spy"
                     /locus_tag="ACHJWB_000573"
     CDS             complement(11829..12314)
                     /gene="spy"
                     /locus_tag="ACHJWB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4312255.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12644..13612)
                     /gene="astE"
                     /locus_tag="ACHJWB_000574"
     CDS             complement(12644..13612)
                     /gene="astE"
                     /locus_tag="ACHJWB_000574"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4312256.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13605..14948)
                     /gene="astB"
                     /locus_tag="ACHJWB_000575"
     CDS             complement(13605..14948)
                     /gene="astB"
                     /locus_tag="ACHJWB_000575"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4312257.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14945..16423)
                     /gene="astD"
                     /locus_tag="ACHJWB_000576"
     CDS             complement(14945..16423)
                     /gene="astD"
                     /locus_tag="ACHJWB_000576"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4312258.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16420..17454)
                     /gene="astA"
                     /locus_tag="ACHJWB_000577"
     CDS             complement(16420..17454)
                     /gene="astA"
                     /locus_tag="ACHJWB_000577"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4312259.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17451..18671)
                     /gene="astC"
                     /locus_tag="ACHJWB_000578"
     CDS             complement(17451..18671)
                     /gene="astC"
                     /locus_tag="ACHJWB_000578"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4312260.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19117..19923
                     /gene="xthA"
                     /locus_tag="ACHJWB_000579"
     CDS             19117..19923
                     /gene="xthA"
                     /locus_tag="ACHJWB_000579"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4312261.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20090..20800
                     /gene="ydjX"
                     /locus_tag="ACHJWB_000580"
     CDS             20090..20800
                     /gene="ydjX"
                     /locus_tag="ACHJWB_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4312262.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20805..21482
                     /gene="ydjY"
                     /locus_tag="ACHJWB_000581"
     CDS             20805..21482
                     /gene="ydjY"
                     /locus_tag="ACHJWB_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4312263.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21497..22204
                     /gene="ydjZ"
                     /locus_tag="ACHJWB_000582"
     CDS             21497..22204
                     /gene="ydjZ"
                     /locus_tag="ACHJWB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4312264.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22204..22752
                     /gene="ynjA"
                     /locus_tag="ACHJWB_000583"
     CDS             22204..22752
                     /gene="ynjA"
                     /locus_tag="ACHJWB_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4312265.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22762..23928
                     /gene="ynjB"
                     /locus_tag="ACHJWB_000584"
     CDS             22762..23928
                     /gene="ynjB"
                     /locus_tag="ACHJWB_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4312266.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLCLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            23901..25436
                     /gene="ynjC"
                     /locus_tag="ACHJWB_000585"
     CDS             23901..25436
                     /gene="ynjC"
                     /locus_tag="ACHJWB_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO4312267.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQHIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25436..26089
                     /gene="ynjD"
                     /locus_tag="ACHJWB_000586"
     CDS             25436..26089
                     /gene="ynjD"
                     /locus_tag="ACHJWB_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4312268.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26156..27463
                     /gene="ynjE"
                     /locus_tag="ACHJWB_000587"
     CDS             26156..27463
                     /gene="ynjE"
                     /locus_tag="ACHJWB_000587"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4312269.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27472..28092)
                     /gene="ynjF"
                     /locus_tag="ACHJWB_000588"
     CDS             complement(27472..28092)
                     /gene="ynjF"
                     /locus_tag="ACHJWB_000588"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4312270.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28179..28586
                     /gene="nudG"
                     /locus_tag="ACHJWB_000589"
     CDS             28179..28586
                     /gene="nudG"
                     /locus_tag="ACHJWB_000589"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4312271.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFTGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28552..28824)
                     /gene="ynjH"
                     /locus_tag="ACHJWB_000590"
     CDS             complement(28552..28824)
                     /gene="ynjH"
                     /locus_tag="ACHJWB_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4312272.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29060..30403
                     /gene="gdhA"
                     /locus_tag="ACHJWB_000591"
     CDS             29060..30403
                     /gene="gdhA"
                     /locus_tag="ACHJWB_000591"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4312273.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30520..31559)
                     /gene="ynjI"
                     /locus_tag="ACHJWB_000592"
                     /pseudo
     CDS             complement(30520..31559)
                     /gene="ynjI"
                     /locus_tag="ACHJWB_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(31687..33648)
                     /gene="topB"
                     /locus_tag="ACHJWB_000593"
     CDS             complement(31687..33648)
                     /gene="topB"
                     /locus_tag="ACHJWB_000593"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4312274.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQVQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33653..34696)
                     /gene="selD"
                     /locus_tag="ACHJWB_000594"
     CDS             complement(33653..34696)
                     /gene="selD"
                     /locus_tag="ACHJWB_000594"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4312275.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34813..35364)
                     /gene="ydjA"
                     /locus_tag="ACHJWB_000595"
     CDS             complement(34813..35364)
                     /gene="ydjA"
                     /locus_tag="ACHJWB_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4312276.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35525..37381
                     /gene="sppA"
                     /locus_tag="ACHJWB_000596"
     CDS             35525..37381
                     /gene="sppA"
                     /locus_tag="ACHJWB_000596"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4312277.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAEFNDPQNRYAFCLTCANVL"
     gene            37548..38564
                     /gene="ansA"
                     /locus_tag="ACHJWB_000597"
     CDS             37548..38564
                     /gene="ansA"
                     /locus_tag="ACHJWB_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4312278.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38575..39216
                     /gene="pncA"
                     /locus_tag="ACHJWB_000598"
     CDS             38575..39216
                     /gene="pncA"
                     /locus_tag="ACHJWB_000598"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4312279.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASLDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39309..40667)
                     /gene="ydjE"
                     /locus_tag="ACHJWB_000599"
     CDS             complement(39309..40667)
                     /gene="ydjE"
                     /locus_tag="ACHJWB_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312280.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(40784..41542)
                     /gene="ydjF"
                     /locus_tag="ACHJWB_000600"
     CDS             complement(40784..41542)
                     /gene="ydjF"
                     /locus_tag="ACHJWB_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4312281.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKIMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(41679..42659)
                     /gene="ydjG"
                     /locus_tag="ACHJWB_000601"
     CDS             complement(41679..42659)
                     /gene="ydjG"
                     /locus_tag="ACHJWB_000601"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO4312282.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKKLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(42669..43616)
                     /gene="ydjH"
                     /locus_tag="ACHJWB_000602"
     CDS             complement(42669..43616)
                     /gene="ydjH"
                     /locus_tag="ACHJWB_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4312283.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(43621..44457)
                     /gene="ydjI"
                     /locus_tag="ACHJWB_000603"
     CDS             complement(43621..44457)
                     /gene="ydjI"
                     /locus_tag="ACHJWB_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4312284.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(44478..45521)
                     /gene="ydjJ"
                     /locus_tag="ACHJWB_000604"
     CDS             complement(44478..45521)
                     /gene="ydjJ"
                     /locus_tag="ACHJWB_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4312285.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(45538..46917)
                     /gene="ydjK"
                     /locus_tag="ACHJWB_000605"
     CDS             complement(45538..46917)
                     /gene="ydjK"
                     /locus_tag="ACHJWB_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312286.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(46944..48020)
                     /gene="ydjL"
                     /locus_tag="ACHJWB_000606"
     CDS             complement(46944..48020)
                     /gene="ydjL"
                     /locus_tag="ACHJWB_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4312287.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(48390..48662)
                     /gene="yeaC"
                     /locus_tag="ACHJWB_000607"
     CDS             complement(48390..48662)
                     /gene="yeaC"
                     /locus_tag="ACHJWB_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4312288.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(48705..49118)
                     /gene="msrB"
                     /locus_tag="ACHJWB_000608"
     CDS             complement(48705..49118)
                     /gene="msrB"
                     /locus_tag="ACHJWB_000608"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4312289.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            49460..50455
                     /gene="gapA"
                     /locus_tag="ACHJWB_000609"
     CDS             49460..50455
                     /gene="gapA"
                     /locus_tag="ACHJWB_000609"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4312290.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            50539..51423
                     /gene="yeaD"
                     /locus_tag="ACHJWB_000610"
     CDS             50539..51423
                     /gene="yeaD"
                     /locus_tag="ACHJWB_000610"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4312291.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFMDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(51474..52328)
                     /gene="yeaE"
                     /locus_tag="ACHJWB_000611"
     CDS             complement(51474..52328)
                     /gene="yeaE"
                     /locus_tag="ACHJWB_000611"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4312292.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(52418..53164)
                     /gene="mipA"
                     /locus_tag="ACHJWB_000612"
     CDS             complement(52418..53164)
                     /gene="mipA"
                     /locus_tag="ACHJWB_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4312293.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTILAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
BASE COUNT        13327 a        13994 c        12789 g        13173 t
ORIGIN      
        1 aaggcagctt tttgttacag gtggtttgaa tgaatgtagc aacgaaatgc agaatttcag
       61 gtcatgtaac tcccggcaaa accgggaggt atataatcct tactcagtca cttccccttc
      121 ctggcggatc tgatttgccc aacgttgggc agattcaggc acagtaaacg ccggtgagcg
      181 cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca tccagacatc
      241 acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc gtgggacata
      301 gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct tcactaaacg
      361 catattaaaa attagaaaaa ctgtagttta gccgatttag cccctgtacg tcccgctttg
      421 cgtgtatttc ataacaccgt ttccagaata gtctccgaag cgggatctgg ctggtggtct
      481 atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt aaggagacga
      541 gttcaatgtc gcaacataac gaaaagaacc cacatcagca ccagtcacca ctacacgatt
      601 ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggatct catcgtccag
      661 cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc tgggagcctg aaagcccctg
      721 atacacgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt gaaaattatg
      781 cgctgaccac taatcagggc gtgcgcatcg ccgatgatca aaactcactg cgtgccggta
      841 accgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc cactttgacc
      901 atgagcgcat cccggaacgt attgttcatg cacgcggatc agcagctcac ggttatttcc
      961 agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat ccgaacaaaa
     1021 tcaccccagt atttgttagg ttctctaccg ttcagggtgg cgctggctct gccgataccg
     1081 tgcgtgatat ccgtggtttt gccaccaagt tctatactga agagggtatt tttgacctcg
     1141 ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc gattttgttc
     1201 atgcggtaaa accagaaccg cactgggcaa ttccacaagg gcaaagtgcc cacgatactt
     1261 tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg gcgatgtcgg
     1321 atcgcggcat cccccgcagt taccgcacca tggaaggctt cggtattcac accttccgcc
     1381 tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca ctggcaggta
     1441 aagcctcact cgtttgggat gaagcacaaa aactcaccgg acgtgacccg gacttccacc
     1501 gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa ctgggcttcc
     1561 agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat ccaaccaaac
     1621 tgatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc aatcgcaatc
     1681 cggataactt ctttgccgaa aacgaacaag cagcattcca tccaggtcat attgttcctg
     1741 gtctggactt caccaacgat ccgctgttgc agggacgttt gttctcctat accgatacac
     1801 aaattagtcg tcttggtggg ccgaatttcc atgagattcc gattaaccgt ccgacctgcc
     1861 cttaccacaa cttccagcgt gatggcatgc atcgtatggg gatcgacact aacccggcga
     1921 attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg ggaccgaaac
     1981 gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgc gagcgcagcc
     2041 catcgtttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag acgccattcg
     2101 agcagcgcca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt cgtccgtata
     2161 ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc caggcggtgg
     2221 cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca cctccggacg
     2281 tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac ggtgatgtga
     2341 aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag atcggcagac cttctggcca
     2401 ttctcaaggc cctgaaggcc aaaggcgttc atgccaaact gctctactcc cgaatgggtg
     2461 aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc ggtgcacctt
     2521 cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc gctgacaacg
     2581 gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt gcgctggcgg
     2641 gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa gaagggattg
     2701 tggaagctga cagcgctgac ggtagtttta tggatgaact gctaacgctg atggcagcac
     2761 accgcgtgtg gtcacgcatt cctaagattg acaaaattcc tgcctgatgg gaggcggcgc
     2821 aattgcgccg cctcaaatga tttacatagt gcgctttgtt tatgccggat gcggcgtgaa
     2881 cgccttatcc ggcctacaaa actgtgcaaa ttcaatatat tgcaggaaac acgtagatac
     2941 tgataagcga agcacatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta
     3001 cgccgccttt gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc
     3061 ctcacttaca catcccgata actccccaac cgataaccac gctgagcgat agcacctttc
     3121 aacgacgctg atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga
     3181 cggatagtat tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga
     3241 tggcttgcgt catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac
     3301 gcgctactga atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc
     3361 ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg
     3421 aaaatctgcg ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca
     3481 aatagctcaa tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt
     3541 aaagcatctt cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac
     3601 cccggcatag ctgtcagtgg cttgcccata gtaaggacaa agtgcatccc tatggccaga
     3661 ctcggttcat cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc
     3721 gtcgtcgatg tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg
     3781 cttaagccaa aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa
     3841 tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc
     3901 agaatcatct cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg
     3961 tttagcgcca gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc
     4021 tcgaagcctt taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc
     4081 ggatgtggcg tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc
     4141 ggaatattat caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg
     4201 ttgtagatag cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt
     4261 tccagttctt tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc
     4321 tcgactttct gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata
     4381 gccagcatct ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac
     4441 gaacgaatta agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt
     4501 aactgccctg acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc
     4561 acatccttaa tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg
     4621 tctttcagca tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg
     4681 ccgataaagc gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg
     4741 ttagtgaagt taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca
     4801 aaaatcaccg gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga
     4861 ccaagataac catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct
     4921 acgcgcagtt gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt
     4981 ttataaagct tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg
     5041 atatccagtt tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat
     5101 tcgtgataac gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca
     5161 atagtgacga cttttaattt ctggctcata atttctccct tcagtacaga atactgatat
     5221 ctggcatatc tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt
     5281 caggtataac gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa
     5341 aggacgttaa tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa
     5401 acgcaatatc cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac
     5461 ggatctcatt aataatctgc attggtgttt tgccataata tcgttgagtc gctcgcgtca
     5521 aatattcctg tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact
     5581 gctctttatc atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca
     5641 cctgttcttc gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa
     5701 actcttccag ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga
     5761 gaaaagcgtt attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca
     5821 ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa
     5881 actcataaaa actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca
     5941 gtaacacgcg cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt
     6001 cataatagtc gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat
     6061 ggaagttttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa
     6121 tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca
     6181 gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc
     6241 agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga
     6301 tgcgcttcat tcaatgccat ccgtgactga tacatcatgg cttttgctgc agcaaaatcg
     6361 ccctgcttcg cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg
     6421 ataagcccca tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta
     6481 tcgagatcca tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca
     6541 tcaattaaaa tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat
     6601 tttgtgcttt gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt
     6661 tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac
     6721 cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg
     6781 ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg
     6841 ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg
     6901 gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca
     6961 ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct
     7021 tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct
     7081 gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca
     7141 gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc
     7201 agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat
     7261 ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa
     7321 taaagccggg aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca
     7381 gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa
     7441 taataatccc ggagatgata ttcaccccac ctaaccagtt tgcgccaacc gcataggcct
     7501 caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac ccagccgcca
     7561 gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt gccattaaag
     7621 acattatccc taatgttccg ttatatacgt tgccgccaat acctttcaga ccattaagtg
     7681 tttcaatggt tgaggtgtcg aggcgaatac ctaaggaata aaaaaacgac ccctccccaa
     7741 agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt aacggcatta
     7801 agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt ttaactgcaa
     7861 aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa tacccttaaa
     7921 aaccgcaatt taaatattgc ggtattgatt tatgaaataa ctctttgacg ggaaaattta
     7981 aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta agcacgccta
     8041 aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca
     8101 aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat
     8161 tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat
     8221 atttttctgc ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg cccgcagaac
     8281 aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc
     8341 cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga aaccaacatg
     8401 actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta tttctttgac
     8461 cttataaatt gtctctcctt gacctgcggt tatgaccctc ttcacatttc gggcaaataa
     8521 ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cacaatggcc ggtttccgtt
     8581 gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt gtcgtattca
     8641 gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc gacaaagtag
     8701 gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc acgagcatgg
     8761 atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc ccggctcatg
     8821 ccttttttga cgtctttcac cacaggctgt acaagctggt ctttagtacg atcataagcc
     8881 gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc catattcttg
     8941 ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac attgggcgct
     9001 ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg acgttttaac
     9061 caccattcag cccgctgtcg cttgtcgttt cagtggcaac gggttagctt taaggaagtt
     9121 ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg
     9181 gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtatcgat tttctgaaaa
     9241 gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg
     9301 actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc ctggaaaccg
     9361 gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag gccgacgaac
     9421 aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca
     9481 agggcgcggt attggccagc gaacaggcat tgcgggaagc aggcattgaa ctgagcgatt
     9541 ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta
     9601 tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca
     9661 ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg
     9721 gtaaacagtt gctgacggca ttaggttgcc cggaacacct ttataagaaa gccccaacgg
     9781 ccgatctgga agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtgacctatg
     9841 acaatatcga cgactatctg gaagggaaaa acgtacctga acaggtcgcc agaacaatag
     9901 agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaccgtt ttcgatgatt
     9961 tctggaaaaa gtaataattt gcacataatg gtttgtgcga aaaagagtaa tttgttcacg
    10021 ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atatattgca
    10081 ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcgaccc
    10141 gcctcttttt taatcgcttc ccgcctgtta cactggatag ataaccagca ttcggagtca
    10201 gcagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa
    10261 tatcccgaac atttacgttc attccttaat gacttaccca cccgaccagg ggtgtatctg
    10321 tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcattaa catccgcagc
    10381 cgcgtccttt ctcatttacg taccccggat gaagccgcca tgctacggca atcccgacgc
    10441 atcagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc
    10501 aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgcgccctg
    10561 caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc
    10621 cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc
    10681 gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt
    10741 gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat
    10801 gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt gtgttggcct
    10861 tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt
    10921 caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg
    10981 acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt
    11041 aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga
    11101 acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg
    11161 cgccctggtc ggtagtgagc aatttatcgc ccagttgcca cgccccgtta atcacaaaca
    11221 ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct
    11281 tacagacatc caggcgtgtc atgatgttga aatccatcga catctgccct gccgtcagtt
    11341 tcgcttttac cacctggtcc gctgcaaagg caaaaggctg taacggtttt aaggtatggt
    11401 taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt
    11461 ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg ctggcacgcc
    11521 agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca ccggcagcat
    11581 ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc
    11641 cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt
    11701 tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg
    11761 atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg
    11821 ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga
    11881 cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat gttgtaaatt
    11941 ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg
    12001 atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg
    12061 cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgcgg
    12121 atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga
    12181 ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg
    12241 tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac aaacagtgca
    12301 gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta
    12361 gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg
    12421 taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt
    12481 gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta
    12541 taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa
    12601 gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga
    12661 gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac acatactcta
    12721 catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa
    12781 acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc gagtggcgag
    12841 taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg acactctcac
    12901 cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc
    12961 caaagggcaa cgctttgcca agttccagcg tacaggccag cgcgccaaaa tgtctggcgc
    13021 tgaaatgggt aaacgtacca ccaggttcct gatggaacac cagcgcctcc agccccgccg
    13081 cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt aatacaccga
    13141 actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccag cgcacagatt
    13201 ctttgcccag gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc cgacaggttt
    13261 ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcgattcata tcgctatggc
    13321 aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc aaccgccaac
    13381 gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatc tccacaggtg
    13441 ccgtctcatt accgtgtatt cccgctgaaa tcaccagtgc gccttgcggt ggcgttaatg
    13501 gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg atttctcgct
    13561 cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc attgccccct
    13621 ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa tacatccagt
    13681 gcttcccgcc ctccgcgcag caattgcggg tcggccagat cggcagcagt aaggcgatcg
    13741 cggtagtaac gatccaccca gtcattgagc gcattaaaca gcgtatcgtt catcatcacc
    13801 gccggattca ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg caggcacgcc
    13861 gggccgccgc cattcgccat gctttcacgt aaatcaaaga cttttagttc gctaatcggg
    13921 ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgttc ccgacactcc
    13981 tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct gttaaacaga
    14041 taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat cgccataaaa
    14101 ccgttcaccc gcgcacgcag gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg
    14161 tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgccctgg
    14221 tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattcagc
    14281 cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta
    14341 tcattgcctt cttctcgccc gtagacaaaa agttgcatac ccggctcacc gtaatgaccg
    14401 ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc
    14461 gaatgaacgc taaatttctc ttcgtcgtta aaaatcgctt ttaaaagtga ttcggtgacg
    14521 tgcgcttcca gcgagcggtg aaatttattg ttcaggttgg caacggtgag atgcactttg
    14581 ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa
    14641 gcggaactga cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc
    14701 tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc
    14761 gggatcacgg cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt
    14821 tttgccgcct gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca
    14881 aacgacaggc cagcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag
    14941 gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag
    15001 ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg
    15061 cgctggggcg atggttaccg gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac
    15121 cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga
    15181 atttttcccg ctcgggggaa accagaccgc aagagagtcc gaagcgagtg ttattcgcca
    15241 ttagaatcgc ttcctcgaaa gaatcataac gccagacgcg caataacggt ccgaacacct
    15301 cttcatctgg tacgccagca acgcctgtca tttcaatgat ccccggcgtc agcaacgatg
    15361 tctctgattg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct
    15421 gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct
    15481 gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc agacgagcaa
    15541 gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt
    15601 gcccggctgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca
    15661 cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct
    15721 gaccggagag ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac
    15781 cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt
    15841 tcagtacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc
    15901 acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga
    15961 tatgtccgtt cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt
    16021 gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac
    16081 gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg
    16141 ccgcttccca gcgcggctta cccgtttctc tggcaataat cgcggttaat tcgcctttat
    16201 tcctttccag caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc
    16261 gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc tgagcggcat
    16321 cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg
    16381 cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc
    16441 gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc
    16501 ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg
    16561 gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc
    16621 tgccacttcc accagccgac ttttacgaat ggcgcgcacg cggtcgatgt cacactcaag
    16681 cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag
    16741 caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg
    16801 ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg
    16861 cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag
    16921 gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac
    16981 tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa
    17041 atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct
    17101 gccggtgtga tcgttactga gaaacagcgt cggcaatgcg ttatagacat tcagctcttt
    17161 tgaggcgtga accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc
    17221 cacctcaatg gcacaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac
    17281 atagccctgc tcacttttgg gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg
    17341 cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc
    17401 aagctgcatc agcgccgaga catctgatcg ctcaacggga cggatgacca tcatgatgaa
    17461 cctcggctaa caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct
    17521 tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc
    17581 atcacgcctg ctttcgccgc ttcctgagag atctgtttcg cttgcccggc gtaatcggca
    17641 ttcagtacac agccaatcag caaacctaag ccgcgaactt cactgaacaa accatagcgg
    17701 tgattaatag tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc
    17761 atctctggtg tgttgatgag ctccagcact ttgcctgcca ccgccgaggc cagcggatta
    17821 ccgccatagg tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc
    17881 aacaacgcac cgaccgggaa accgccgccc agcgctttgg cggtagttaa cagatcaggc
    17941 gtcacgccgt agtgcatata ggcatacagt tccccggtgc gcccgacgcc ggtttgtact
    18001 tcatcaaaaa tcaacagcgc attgtggcgg ttacacaatt cacgcagacc ttgtaaaaac
    18061 gcgttgctgg ctggcaccac accgccttcc ccctggatgg gttcgacaat caccgcacag
    18121 gtagagtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata
    18181 tccgccggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta
    18241 aacagcgtgc gaccatgaaa cgcatttttg aacgccacga tgccgctctt atggctgccg
    18301 tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg
    18361 gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc
    18421 agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc
    18481 agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg
    18541 aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt
    18601 ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt
    18661 ggctgagaca tagcgacctc tacagcaaat aattcgttat ttatatgtta acaataagta
    18721 atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa
    18781 acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca
    18841 tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg
    18901 cagaagtaaa atataattct ggaattgtga tcattgacga aatttactgg aaattactgc
    18961 gccattctga cgcagcgcgc accaaaagcg ggcatttttt gcgccatcgt tgacatcatt
    19021 aacaaccatc gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca
    19081 tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc
    19141 aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat
    19201 gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg
    19261 aagctcggct acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg
    19321 accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag
    19381 cggcggatta ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt
    19441 tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt
    19501 tatcagaatc tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt
    19561 atgggcgata tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt
    19621 aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac
    19681 aggctgatga gctgggggtt ggtcgatact ttccgccatg ctaatccgca aacagcagat
    19741 cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc
    19801 gacctgctgc tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat
    19861 gaaattcgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc
    19921 taatttagca gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa
    19981 caaactgtca gtaatgattt gttgcctgcc gtcgtttgtt ataccgtcac tgcgttttta
    20041 gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga
    20101 gcgtaacttt ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt
    20161 cggtttattc gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg
    20221 attgttcggt tatagcctct atattctgtt attcatcatt gccaccctct ttctgttacc
    20281 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag ggacactact
    20341 ctcattaatt gccgcaacgc tggcctcctc gagctcattc ctgctggcgc gctggctggg
    20401 acgtgattta ctgctgaaat acgtgggcca tagccatacc ttccaggcca ttgaaaaagg
    20461 cattgcgcgt aacggtattg attttcttat tctgacccgc ttaatcccgt tgtttcctta
    20521 caatattcaa aattacgctt acggattaac caccatcgcc ttctggcctt atacccttat
    20581 ttcggcactc acgaccctgc ccggtattgt tatttatacc gtgatggcaa gcgatctcgc
    20641 caatgaaggc attacgctgc gctttatttt acaactctgt ctggcgggcc tggcgctgtt
    20701 tattctcgtc cagctcgcaa aactctacgc ccgacacaaa catgtggatc tgtctgcttc
    20761 gcgccgcagc ccacttactc acccaaaaaa tgaaggatag aacgatgtcg caacattatt
    20821 cagtgtcatg gaaaaaagga ctggctgcac tctgcttact ggctgttgcc gggcttagcg
    20881 gctgcgatca aaaagagaat gcggcgggaa aagtggaata cgacggactt tcgaacagcc
    20941 aaccgctgcg cgtcgatgcc aataaccata cggtaaccat gctggtgcaa attaatggtc
    21001 gtttcctcac cgacgacact cgtcacggta ttgtgtttaa agatggctcc aacggacata
    21061 aatcgctgtt tatggcttat gcgaccccga aagcatttta tgaagccctg aaagaggcag
    21121 gtggtacgcc gggcgaaaac atgacgatgg ataataaaga aacgactctt gtcacaggca
    21181 gcaaactgga tatttcggtc aactggcaag gggcggcaaa agcgtattcc ttcgatgaag
    21241 tgattgttga cagtaatggc aagaaactgg acatgcgctt tggcggtaat ttaacggcag
    21301 cagaagagaa gaaaacgggt tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca
    21361 gcaatgcaac atacacttat ggtgcggtgg aaaaacgcgg cgaagtgaaa ttcaaaggca
    21421 atgcctcagt tctcccggcg gataacacgc tggcaacggt tacctttaaa atcaccgaat
    21481 aaagccagga taaaggatga tgaagataca atcgcgtaaa atctggtact accgtataac
    21541 cctcatcatc ctgttgttcg ctatgctgct cgcatgggcg ctgcttcccg gcgtccatga
    21601 gtttatcaat cgcagcgttg cggcgtttgc cgccgtggac caacagggta tagaacgctt
    21661 tattcagtct tacggcgcac tggcggcagt tgtctcgttc ttgttgatga ttttacaggc
    21721 cattgctgca ccgctacctg cgtttttgat cacctttgcc aatgcgtcgc tgtttggcgc
    21781 gttctggggc ggcttgctgt cgtggaccag ttcgatggcc ggcgcggcgc tgtgcttttt
    21841 tatcgccaga gtgatgggcc gcgaagtggt ggaaaaatta accggcaaaa ccgtgcttga
    21901 cagtatggac ggctttttca ctcgctacgg caaacacacc atcctggtct gtcggttatt
    21961 gccttttgtc cctttcgatc caatcagcta tgctgccggt ctgacttcaa tacgttttcg
    22021 ctcgtttttt atcgccaccg ggcttggtca attaccggcg actattgttt attcctgggc
    22081 gggcagcatg ttaacaggcg gtactttctg gtttgtcacc ggactgttta ttctgtttgc
    22141 cctgaccgtg gtgattttta tggcgaagaa aatatggctt gagcgccaga agaggaatgc
    22201 ctgatgggtt taccgccgct tagcaaaatt cctttcattt tacgtccgca ggcgtggctg
    22261 catcgtcgcc attacggcga ggtgctaagc ccgattcgct ggtgggggcg gatcccgttt
    22321 atcttttatt tggtgtcaat gtttgttggg tggctggagc gcaaacgctc accgctcgat
    22381 ccggtagtgc gatcgcttgt cagcgcgcgc attgcgcaaa tgtgcctgtg tgagttttgc
    22441 gtggatatca ccagtatgaa agtcgccgag cgtaccggca gcaccgataa actgctggca
    22501 gtggctgact ggcggcaaag cccgctcttt agcgatgaag aacggctggc gctggagtac
    22561 gccgaagccg caagcgtcac accgccaaca gtcgatgatg ccctgcgtac ccgactggct
    22621 gcgcattttg acgctcaggc gctcaccgaa ctgacggcat tgatcggcct gcaaaacctg
    22681 tcggctcgtt ttaattctgc catggacatt cccgctcagg ggctgtgccg tattcctgaa
    22741 aaacgttctt aaggagagat gatgcgccat tgtgggtggt tgctgggatt gttatcgctg
    22801 ttttctttgg caacacatgc cagtgactgg caggaaatta aaaatgaggc caaagggcaa
    22861 accgtctggt ttaacgcctg gggcggcgat accgcaatta accgctatct cgactgggtt
    22921 agcggcgaga tgaaaaccca ttacgctata aacctgaaga ttgttcgtct cgcggatgcc
    22981 gcagatgcgg tgaagcgcat tcagaccgaa gctgctgccg gtcgtaaaac gggcggctcg
    23041 gtggatctgc tctgggtgaa cggcgaaaac ttccgcacct taaaagaggc caatttatta
    23101 caaacgggct gggcggagac tctgcccaac tggcgctatg tcgacacaca gctgccggtg
    23161 cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc cctggggcgg cgcacaactg
    23221 acgtttatcg cccgccgcga tgttacgcca cagccaccac aaacgccgca agccttactg
    23281 gagtttgcta aagccaatcc cggcacggtt acttatccgc gcccaccgga ctttaccggc
    23341 acggcgtttc ttgaacagtt gctgattatg ctgacgccag atcccgccgc attaaaagaa
    23401 gcgccggacg atgcgacttt cgcccgtgtc actgcgccct tgtggcaata tcttgatgca
    23461 ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc cttcgcccgc gcggatggat
    23521 gctctgctga aagccggcac attgtgcctg tcgctgacct ttagccccgc gcatgcgcag
    23581 caaaaaatcg ccagcggcga tttgccggca agcagttaca gttttggctt tcgcgagggg
    23641 atgataggca acgtgcattt cgtcaccatt ccagccaacg cgaatgccag tgctgcggcg
    23701 aaggtagttg ccaatttcct gctctcaccc gatgcgcaac tgcgtaaagc agatcccgtt
    23761 gtctggggcg atccttctgt tctcgatccg caaaaactgc ctgacgggca gcgcgaaata
    23821 ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg ctgaaccgca cgcaggatgg
    23881 gtgaatgcac tggaacaaga atggttacgc cgttacggta cgcattaatc tttttgctgt
    23941 gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc agctctcacg ctgatatcgc
    24001 ctgcgttgtc gttgacacac tggcaggcgt tatttgccga tccacagtta ccgcatgcat
    24061 tactggcaac gctggtgtcg acaaccatcg cggcggtcgg ggcattgttg attgccctgc
    24121 tggtgattgt ggcgctatgg cctgggccga aatggcagcg tatgtgcgcc cgtctgccgt
    24181 ggctgctcgc cattccccat gtggcttttg ccaccagcgc cctgctgctc tttgctgacg
    24241 gagggctgct atatgactat ttcccgtatt tcactccgcc aatggaccga tttggcatcg
    24301 gtctgggcct cacccttgca gtgaaagaaa gcgcatttct gctgtggatc ttagcggcag
    24361 tattgagcga aaaacggctg ttgcagcagg tcattgtgct ggattcgctg ggctacagcc
    24421 gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc gccttcgctg gcaatggcga
    24481 tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt ggcgattatt cttgggccag
    24541 gtaatccacc gacgctggca gtaattagct ggcagtggtt aactcagggc gacgcggatc
    24601 aacaaacgaa aggcgcactc gccagtctgc tgctgatgct gttactcgcc gcctacgttt
    24661 tgctgagcta tctgctatgg cgcagctggc ggcgcactat tccccgcgta gatggcattc
    24721 gcaagcctgc cacaccttta ttaccaggca ctacgctggc gagtttttta cccttaaccg
    24781 gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca gtcgacgatc aatagtgaag
    24841 cactcatcaa cagtctgaca atggggctgg tagcaacatt catcgctttg ctcctgttac
    24901 tgctgtggct ggaatggggg ccacagcgtc gccagttgtg gctatggttg cccattctat
    24961 tgcccgctct gccactggtg gcgggccagt acacgctggc gctatggctg aacctggatg
    25021 gaagctggac ggcggtggtc tgggggcatc tgctgtgggt aatgccgtgg atgctgttta
    25081 tcctgcaacc cgcctggcag cacattgatt cacggttaat tttgattgcg caaacactgg
    25141 gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc actcatgttg cgccctgtgc
    25201 tgattgcctt cgcggtggga ttttcagtca gtattgcgca gtatatgccg acgctgtggc
    25261 tgggcgcggg gcgttttccg acgctcacca ctgaggcggt ggcattaagc agcggcggca
    25321 gcaacggtat tctcgccgcc caggctttat ggcaactgtt gttaccgctt attatttttg
    25381 ccctgaccgc gttagtcgca aaatgggtag gttatgtcag acaaggactc cgctaatgct
    25441 ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc ttgctgacaa acgttaactt
    25501 tacggttgat aaaggtgaca tcgtcacgtt aatggggccg tctggctgtg gaaaatccac
    25561 tctgttttca tggatgattg gtgcactggc tggacagttt tcttgtacag gtgagctatg
    25621 gctcaatgag caacggattg acatgctacc caccgcacag cgtcagattg gcattctttt
    25681 ccaggatgca ctgttatttg accagttcag cgtcgggcaa aatttactgc tggcgctacc
    25741 gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa gacgcgcttg atcgagcggg
    25801 actggctgaa acttatcacc aggaccctgc gacattatca ggcggtcagc gagcgcgggt
    25861 ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta ctcctggatg agccattcag
    25921 ccgtcttgat gtggctctgc gcgataattt tcgccagtgg gtgttcagcg aagttcgcga
    25981 actggcgatc cccgtcgttc aggtaacgca cgatctccag gatgttcctg ctgatagttc
    26041 tgttctggat atggagcagt ggtcagaaaa ttacaacaaa ctgcgataac gcaaagtttt
    26101 tctcaatgcg tcagttcaga atggcgcact caaaactaca atgtcgggat tttcgatgaa
    26161 acgtgtttct caaatgaccg cgctggcaat ggctttaggg ctggcttgcg cttcttcgtg
    26221 ggccgctgaa ctggcgaagc ctcttacact tgaccagctt caacaacaaa atggcaaagc
    26281 gatagatact cgccccagcg cgttttataa cggctggcca caaaccttaa atggcccttc
    26341 tggtcatgaa cctgccgcct taaacctctc tgccagctgg ctcgacaaaa tgagcaccga
    26401 acagctcaac gcgtggatca agcaacataa cctgaaagcc gatgccccgg tggcgctgta
    26461 cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg caaaaagcag gttttacgca
    26521 tatctccatt ctgagtgacg cgctaagcga accttcccgt ctacaaaaac tgccgcactt
    26581 tgagcagctg gtttatccgc aatggctgca cgacctgcaa caaggtaaag aggttacggc
    26641 gaaacctgcc ggtgactgga aagtcattga agcggcctgg ggcgctccta agctttacct
    26701 tatcagccat attcccggtg ctgactacat cgataccaac gaagtggaaa gcgaaccgct
    26761 gtggaacaaa gtttctgatg aacaactaaa agcgatgctg gcaaaacacg gcattcgcca
    26821 tgacaccacg gtcattctgt acgggcgtga cgtatacgct ggagcgcgtg tggcgcaaat
    26881 tatgctctac gcaggcgtga aagatgtgcg tctgctggat ggcggctggc aaacctggtc
    26941 cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa gtgaaagcgg aaccggattt
    27001 cggcgtgaag atcccggcac aaccgcagtt gatgctcgat atggaacagg cccgtggact
    27061 gctgcatcgc caggatgcat cgctggtgag cattcgttcg tggccagaat ttatcggtac
    27121 gaccagcggt tacagctata ttaaaccaaa aggtgaaata gccggagcac gttggggaca
    27181 cgctggtagc gactcgacgc atatggaaga ttttcataac ccggatggca ccatgcgcag
    27241 cgccgatgat attaccgcta tgtggaaagc gtggaatatc aaaccagagc agcaagtttc
    27301 attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt atgtacgcac gcgccatggg
    27361 ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa tggagcagcg atccaaaaaa
    27421 tccggtagca accggtgaac gcggcccgga cagtagcaaa taacattgca attactgacg
    27481 ctggagcgac ttcagcgtca gatacccact ccacacccgc gtaaaggtcg tcatccagca
    27541 caacgcaccg aagatccacg caaaccacgc aaaccatgca gggaataaac atcccagcac
    27601 aaacagtaag atcgtttctg tgccttcggt taacccgccc agataataaa acgatttatg
    27661 cgcataaccg ggattatcaa tctgatgttt cgccgccagc gcagcaaaag cgagaaaact
    27721 gctgcctgtg ccgataaacg caaacagcag ccagccgcct gccagggcgt tttgctgcgg
    27781 tgcggcgagg ataaacccaa aaggtaccag cgcgtaaaaa agaaaatcga gagaaatatc
    27841 aagaaagccg cccgcgtcgg taagccctct gcgccgcgcc agcgcaccgt ccagaccatc
    27901 aagcagcctg ttcaacaaaa tgacgacaag cgccgccaga taccagccca gcgccagaaa
    27961 aggcagtgcc agcacgccga tggcaaatcc cactaatgtt aaaccatctg gcgtaatgcc
    28021 cggtttatca agaacccgca cgcactgatg caacaacggt ttaatccggg gatgaagatg
    28081 gcggtctagc acaggcactc cttaaatata aagcctttct gattgagcaa cagtgcggat
    28141 attatggcat ttttcgctta tctgcccgtg tgtaatttat gaaaatgatt gaagttgttg
    28201 ccgccatcat tgaacgtgat ggcaaaattt tactcgcgca acgccccgcc cagagcgatc
    28261 aggcgggatt atgggagttt accggtggta aagtcgagcc ggatgaaagc cagcggcagg
    28321 cgctggtgcg tgagttacgc gaagaactgg gcatcgaagc aactgtgggt gagtatgtta
    28381 ccagccatca gcgagaagtt tcgggccgga ttatccatct tcatgcctgg cacgtacccg
    28441 acttccacgg gacgttacag gcacatgaac atcaggcgct ggtctggtgc tcacctgaag
    28501 aggcgctgca atatccgctg gcccctgctg acattccatt attagaggcg tttatggctt
    28561 tacgcgccgc cagaccagcg gattagtgct aagggttttg tcatcccgct ggcattgcag
    28621 cagtattccc tcggctttaa ttaccgcgcc ttcagaataa tttttatcct gataaacgca
    28681 gcactgagta cagggctgcg ctgactgccc gcctgaactg aacacttctg gcggtacgtt
    28741 tacctccacg tccggacgat aatgcgggtt agccagtgca attaatggaa atgctaatac
    28801 tacggcgaac aatgctcgac tcacagggaa ctccttaacg ttattgtctc tgctactgat
    28861 aacggtagcc gggtggcaaa actttagcgt ctgagttatc gcatttggtt atgagattac
    28921 tctcgttatt aatttgcttt cctgggtcat ttttttcttg cttaccgtca cattcttgat
    28981 ggtatagtcg aaaactgcaa aagcacatca cataaacaac ataagcacaa tcgtattaat
    29041 atataagggt tttatatcta tggatcagac atattctctg gagtcattcc tcaaccatgt
    29101 ccaaaagcgc gacccgaatc aaaccgagtt cgcgcaagcc gttcgtgaag taatgaccac
    29161 actctggcct tttcttgaac aaaatccaaa atatcgccag atgtcattac tggagcgtct
    29221 ggttgaaccg gagcgcgtga tccagtttcg cgtggtatgg gttgatgatc gcaatcaggt
    29281 acaggtcaac cgtgcatggc gtgtgcagtt cagctctgcc atcggcccgt acaaaggcgg
    29341 tatgcgcttc catccgtcag ttaacctctc cattcttaaa ttcctcggct tcgaacaaac
    29401 cttcaaaaat gccctgacta ccctgccgat gggcggtggt aaaggcggca gcgatttcga
    29461 tccgaaagga aaaagcgaag gtgaagtgat gcgtttttgc caggcgctga tgactgaact
    29521 gtatcgccac ctgggcgcgg ataccgacgt tccggcaggt gatatcgggg ttggtggtcg
    29581 tgaagtcggc tttatggcgg ggatgatgaa aaagctctcc aacaataccg cctgcgtctt
    29641 caccggtaag ggcctttcat ttggcggcag tcttattcgc ccggaagcta ccggctacgg
    29701 tctggtttat ttcacagaag caatgctaaa acgccacggt atgggttttg aagggatgcg
    29761 cgtttccgtt tctggctccg gcaacgtcgc ccagtacgct atcgaaaaag cgatggaatt
    29821 tggtgctcgt gtgatcactg cgtcagactc cagcggcact gtagttgatg aaagcggatt
    29881 cacgaaagag aaactggcac gtcttatcga aatcaaagcc agccgcgatg gtcgagtggc
    29941 agattatgcc aaagaatttg gtctggtcta tctcgaaggc caacagccgt ggtctgtacc
    30001 ggttgatatc gccctgcctt gcgccaccca gaacgaactg gatgttgacg ccgcgcatca
    30061 gcttatcgcc aatggcgtta aagccgtcgc cgaaggggca aatatgccga ccaccatcga
    30121 agcgactgaa ctgttccagc aggcaggcgt actgtttgca ccgggtaaag cggctaatgc
    30181 tggcggtgta gcaacgtcag gccttgaaat ggcgcaaaac gctgcgcgcc tgggctggaa
    30241 agccgagaaa gttgacgcac gtttgcatca catcatgctg gatatccacc atgcctgtgt
    30301 tgagcatggt ggtgaaggtg agcaaaccaa ctacgtgcag ggcgcgaaca ttgccggttt
    30361 tgtgaaggtt gccgatgcga tgctggcgca gggtgtgatt taagttgtaa atgcctgatg
    30421 gcgctacgct tatcaggcct acaaatgggc acaattcatt gcagttacgc tctaatgtag
    30481 gccgggcaag cgcagcgccc ccggcaaaat ttcaggcgtt tatgagtatt taacggatga
    30541 tgctccccac ggaacatttc ttatgggcca acggcatttc ttactgtagt gctcccaaaa
    30601 ctgcttatcg taacgataac acgcttcaag ttcagcatcc gttaactttc tgcgatagca
    30661 gcagatatgc cagtaaagaa atcccattag actatttttt tgataatctt cttcgctttc
    30721 gaacaactcg tgcgcctttc gagaggcaag cattatataa tgccaggcca gttcttcttc
    30781 aattgtcccg ttttgaaaag ctgtgctcga tatcgagatc atccatgata attccgccgc
    30841 ccatattagc ttcgccgagg atttaccgga gctatgatta gcgcaatcag agatatagtc
    30901 tgagggaaaa acagcaaatt tattcaacaa ggcgataacc tgctctgggg cttcctccat
    30961 gttcgcttta aaggtattgg ctccatggtc gccagaaaga aaatgctcca ttaaggcaca
    31021 ataactttcg ctatcttcga taccccattg atcctctaaa gactcgcgtc ttttacttat
    31081 gatatcgatc gagtcaaagg gaagcacatg atattggaag gtatctttgc caggttcagg
    31141 ctttcgcggc cagaactcca gcgtttcaga ccattgctta tgatagaacc gataaggtgc
    31201 gatcaattgt agcgcctgta acttctcgat actgagcggc tcaatacctt tagcctgata
    31261 ataatgcagt tgttcttttt ttgctttaaa accggcccga acaataagcc ccatcataat
    31321 taatagataa agaaaagagc atcccgcagt aatcaggcct ctttcattca aaccgttgga
    31381 tgttatcgct gcgaatacaa acattacagc gacaacacat gttaaatacc cccccactta
    31441 caaagcagca tggccttatt ttctttaatc atccgttcaa aattactatt aaatatttcc
    31501 cagccattaa aagaatactt ctcgctccca ggatggtttt gtaataaaac ttttttcatc
    31561 acattccctg ttattacata ctttagaggc gataatttta tccatgcaaa aaaaatatcc
    31621 aacgaatttt cttgatccgc tggatatttc agaatatgac tcgaatagca cgaaagattc
    31681 gctcgcttac gctatcgccc cgcttccggc ttcatctgct ggcggacttt ttttcgcact
    31741 acgtttacgc ggtgcagcct ttttcttatc agcactgcca ccactgcccg gagccacaat
    31801 gccgcgaaac tgccgcaccg gagtacgttt ggcttgatca ataagctgat atagcgtccc
    31861 caccagcggc tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac
    31921 cgattcccag tgcgcggtca tgtccggtcg cgtagccatt tctggcagcg aatggaataa
    31981 cgcttttccg gcgtcggtgg agtggatata gcgccctttt ttggtgagga aaccacgctt
    32041 gaacaacagt tcaataatcc cggcacgcgt tgcttctgtc cccagaccat cggtcgcacg
    32101 aaggatcttt ttcagatctt tgtcctgcac aaagcgcgca atcccggtca tcgccgaaag
    32161 cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt
    32221 ttcacacagt aactcatcgc ctttcgccac cacaggcaat ggcgtgccgt cgttttcttc
    32281 atcgcgctct ttgctgccta acagcgcgcg ccaacctgct tcagcaagaa aacgcgcttt
    32341 agcgacaaat ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc
    32401 cgggcagaac tgcatcagat actgacgggc aatcaggtta tagaccttcg cttcgttctc
    32461 cgtcaggttg atcgcagaac tccgtgcggt cggaatgatg gcgtggtgcg catcgacctt
    32521 tttgtcatcc caacagcggt tgcgtatatc tggatctacc actggctgcg gcaacagatc
    32581 tggtgcatga acgctgatgg cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg
    32641 caaatagcga caatcggaac gcggataagt gattaacttg tgtgtttcgt acagcttctg
    32701 gcagatatca agcacgttct gcgcgctcag accaaagcgt tttgccgctt caatctgcaa
    32761 cgccgaaagc gaaaacggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt
    32821 gacaatagcc ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa
    32881 caagcgccct tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt
    32941 aaaccgctca tcggcaggtg tcacgatatg cgctttgact tcaaagaagt ctttcgccac
    33001 gaagttttca atctcttcat cgcggcgcac caccagcccg agcacgggtg tctgcacgcg
    33061 tcccacggaa agtacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt
    33121 catattgatg ccgtacagcc agtcggcacg cgctcgcgcc agcgcagaaa cgcacaatgg
    33181 cacaaactca ctgttggaac gaagacggtc gatcgcccgc tcaaccgcct gcgggttcag
    33241 gtcgtttatc aagcaacgct gcacctgctg gcgcttttcc ggtgccagtt gcagatagtc
    33301 cagcacttca tccaccagca attgcccttc acgatccggg tccccggcgt gaacgatttc
    33361 gctggcttca tgcaggaagc gtttgatgac gttaagttgt ttggtcacgg agggtcgggg
    33421 ctgtaattgc cacttttccg ggacaatcgg caaatccgca agattccagc gcgcatagcg
    33481 gctgtcgtag gcgtctggct gcacctgctc aagcaggtga ccgatacacc aggtcaccac
    33541 ctgaccatta ccgcactcga taaagccatc gcctttacgg tgcggtttgg gcaggacatc
    33601 agcaatggcg cgcgccagac tcggtttttc ggcaataaac aaccgcattg aattaacgga
    33661 tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc gccaatcgcc gtcagttcaa
    33721 tgccaaactc ggcggctgta gttttaacct cattttctgc ttccggcatg accgccagca
    33781 gcaaaccgcc agaagtttgc ggatcgcaca gcagatcgcg cacttcacgc ggcatttcac
    33841 ccatcagatg accgtagctg gcaaagttac gttcggtgcc gccaggcact gcgcccaact
    33901 taatgtactc ttcaaccccc ggcaattttg ggatcgcgtc atagtcaacg cgcgcctgca
    33961 cacctgcgcc ctgacacatt tcgctcaaat ggcccagcag accaaaacca gtaacgtcgg
    34021 tcatcgcttt tacgccttcg atgttggcaa aggacgcacc tgcgatgttc atccggcaca
    34081 tcacttccgt cgccagtccc tgatgttctg gtttcaacag tgattttttc tcagccgtgg
    34141 taagaacgcc gatccccagc ggtttcgtca ggaacagttt gcatccggct tgtgcggtac
    34201 tgtttttctt cacccgctcg gtcggtacga tccccgttac cgccagacca aaaatcggct
    34261 ccggcgcatc gatggagtga ccgccagcca gcgcaatacc cgcctgacgg catgcatagc
    34321 gtccaccttc ggttacttcg cgggcaattt ctggggaaag tttgttaatc ggccagccga
    34381 ggatcgcaat cgccataatc ggtttgcccc ccatcgcgaa gatatcgctg atggcgttag
    34441 tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat cggcataaag aagtcggtgg
    34501 tactgataac gctggtgcca ttgcccagat cgtacaccgc cgcatcgtcg cgggtctcat
    34561 tacccacaag caaattcgga tcaacaaact tcgcctgctc actgtgcagg atggtttcca
    34621 acacttttgg ggaaatttta cagccgcaac cagctccgtg gctgtattgg gtcaaacgaa
    34681 tcgagttctc gctcatggac atctcctgtc aatgcaatcc gggtatggta accctcattc
    34741 cgtgaagtga taagtgagag tgtctgaatt cctgcgcctt tgctcacaat ccagacagtt
    34801 tcgcgacaat tatcagaaat aagttacaaa cggcgtcggg tccgggacgt taatcgacgt
    34861 agatgctttc aactgcggcg tgccgaggta gagaaaaccg acaattttat cctgctcacg
    34921 gcaaccgaat gcttcacgca ctaccggact ttcagttaat gcgccactgc gccagatgcc
    34981 gccaaatccc tgggcaacgg ctgccatttg catcgccatg accgcgcatc cggcagacat
    35041 ttcctgttcc catcgcggga ctttatgatt ttcttcgcat ttcgccacca ccgtgatgat
    35101 gagcggtgcg cggaacggcg cattacgggc tttgtcgata gctttgtcat cactaccggc
    35161 agcaatcgcc ccctgttcca gtacggcgct gaaacgctcg cgcccttccc cttcaatcac
    35221 aaaaaaatgc cacggttgca tggacttatg gtccggcgca cgcatacccg cacgcaggat
    35281 gttttgcagt tgttcacccg ttggcgcggg ttcagccagg cgggaggcgc tacggcgatt
    35341 gatcaatagt tcgagtgcat ccatttgatt aactcctgtc gtgatattta ttcacaaaat
    35401 taacacgaga gtggattttg ttacagcaca gtccgcaatt cctgctgaca agtgccggtt
    35461 gggtcattac gataaccaca tctattgcgc ctgtgacagg tgtgacctta agttgggaga
    35521 atacatgcga accctttggc gatttattgc cggatttttt aaatggacgt ggcgtctgct
    35581 gaatttcgtc cgtgaaatgg tacttaacct gttctttatt ttcctcgtac tggttggtgt
    35641 ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa acggccagtc gtggcgcact
    35701 gctgctggac atttctggtg tgatcgtcga taaacccgac agttctcagc ggtttagtaa
    35761 attaagccgc cagctgcttg gtgccagttc cgatcgtctg caggaaaact cactgtttga
    35821 tatcgtcaac accattcgcc aggcgaagga cgaccgcaat atcaccggta tcgtgatgga
    35881 tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag tacatcggca aagccctgaa
    35941 agagtttcgt gacagcggga aaccggttta tgccgttggc gagaactaca gccaggggca
    36001 atattatctc gccagtttcg ccaataaaat ttggctgtca ccgcaaggcg tggtggattt
    36061 gcacggtttt gctaccaacg gtctgtacta caaatcgttg ctggataagc tgaaagtttc
    36121 cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt gaaccgttta ttcgtgatga
    36181 catgtcaccg gcagcccgcg aagctgacag ccgctggatt ggtgagctgt ggcaaaacta
    36241 tctgaatact gttgccgcta accggcagat ccctgctcag caggtattcc ctggcgcgca
    36301 agggttgctt gagggtttaa ccaaaaccgg tggcgatacc gcgaaatatg cactggaaaa
    36361 caagctggtc gatgcactgg catcgagtgc cgaaatcgaa aaagctctga ccaaagagtt
    36421 cggctggagt aagactgata aaaattatcg cgccatcagt tattacgatt acgcattgaa
    36481 aacgccggca gataccggtg acagcatcgg tgtcgtcttc gctaatggcg caattatgga
    36541 tggcgaggaa actcagggga atgttggcgg tgataccact gcggcacaaa tccgcgacgc
    36601 tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt aatagcccag gcggcagcgt
    36661 taccgcgtct gaagtgattc gcgctgaact ggcagcagcc cgggcagcgg gtaagcctgt
    36721 ggttgtatcg atgggcggca tggcggcatc tggtggttac tggatttcca cgccagctaa
    36781 ttacattgtg gctaacccca gcaccctgac cggttctatc ggtatcttcg gcgtgatcac
    36841 caccgtagaa aatagtctgg attcgattgg tgttcatact gatggtgtct caacttcacc
    36901 gctggcggat gtttctatca ccagggcact gccgccggaa gcgcagcaga tgatgcaatt
    36961 aagcattgag aatggctata aacgctttat cacgctggtt gctgatgcgc gtcattcgac
    37021 gccggagcaa attgataaaa tcgcccaggg ccacgtctgg accggtcagg atgcaaaagc
    37081 taacgggctg gtcgatagtc tcggggattt cgatgatgcg gttgccaaag cagcagagct
    37141 cgcaaaagtg aaacagtggc atctggaata ctacgttgat gaaccgacct tcttcgacaa
    37201 agtgatggac aacatgtctg gttctgtccg ggcaatgttg ccagatgcgt tccaggccat
    37261 gttacctgca ccgcttgcct cggtagcctc taccgttaaa agtgaaagcg acaagctggc
    37321 cgagtttaac gacccacaaa accgttatgc gttttgcctg acctgcgcca acgtgcttta
    37381 agtcttgtac tgagtggccg acagatcgtc ggccacatta ttttttacct cgacgaatcc
    37441 tcttcccgct gtctcgcccc atatttcctt atccgcatag tatcaggtgc gctcctcctg
    37501 cctcacgtat atacttttgc tctttcgata tcattcatat caatatcatg caaaagaaat
    37561 caatttacgt cgcctacacg ggcgggacca tcgggatgca gcgttccgag cagggttata
    37621 ttccggtgtc aggtcatcta caacgccaac tggcgctgat gccggaattc catcgcccgg
    37681 agatgccaga tttcaccatt catgaatata cgccgttgat ggattcttct gatatgacgc
    37741 cggatgactg gcagcatatt gctgaagata ttaaagcgca ctatgacgac tatgatggtt
    37801 ttgtcattct acacggtacc gacacgatgg cgtataccgc ctctgcgctg tcgttcatgc
    37861 tcgagaatct cggtaaaccg gtcattgtga cagggtcaca aatcccgcta gctgagttac
    37921 gctctgacgg acaaattaat ctgctgaatg cgttgtacgt tgcggcgaat tatccgatca
    37981 acgaagtaac gctctttttc aataaccgat tgtatcgcgg caaccgcact accaaagccc
    38041 atgccgatgg ttttgatgcg tttgcctctc caaaccttcc tccgttactg gaagcaggta
    38101 tccatattcg tcgtttgaat acgccacccg ccccgcacgg tgaaggggaa ttaatcgttc
    38161 atccaatcac gccacaacca attggcgtag tgacgattta tccggggatt tctgctgatg
    38221 tcgtgcgcaa ttttctgcgc caaccggtga aagcattgat tctgcgctcc tatggcgtgg
    38281 gtaatgcgcc acaaaacaaa gccttcctgc aggaattaca agaagccagc gatcgcggta
    38341 ttgtggtggt caacctgaca caatgtatgt ccggtaaagt gaatatgggt ggttatgcca
    38401 ccggtaacgc cctcgcccat gccggcgtta ttggcggtgc agatatgact gtagaagcca
    38461 cgctaaccaa actgcattac ctgctgagcc aggaactgga tactgaaacc attcgcaagg
    38521 ccatgagcca aaacctgcgc ggcgaactga cgccggatga ttaaggagac tgcaatgccc
    38581 catcgcgccc tgttactggt cgatttacaa aatgatttct gtgctggtgg cgcgctcgcc
    38641 gtaccggaag gtgacagtac ggtggaggtt gctaaccgcc ttattgactg gtgccagtcg
    38701 cgcggagaag cggttatcgc cagcctggac tggcatccgg cgaatcacgg cagttttgcc
    38761 agccagcacg gtgtagagcc ttatacgcca ggccaactcg acggtttgcc acaaaccttc
    38821 tggccagatc actgtgtgca gaacagtgaa ggcgcacaat tacatccgtt actgaaccaa
    38881 aaagcgatcg cagcggtgtt ccataaaggc gaaaatcctt tagttgacag ttacagtgcc
    38941 ttttttgata acggccgtcg gcagaaaacc tctctcgatg actggttacg cgatcatgaa
    39001 atcgatgaat tgatcgttat gggcctggct actgactatt gcgtgaagtt taccgtgctg
    39061 gacgcgttac agttaggtta taaggtgaac gtgattaccg atggttgtcg tggcgtgaat
    39121 atccagcccc aggacagtgc acacgcgttt atggagatgt cagcagctgg ggcaacgcta
    39181 tatacgctgg cagactggga agagacacag gggtaatttt acgctggcct acaattctgt
    39241 actggcattg taggccaaat aaaacacgtc agtggtacat ctggcaattg atgccatcaa
    39301 cgaaagattt aattcacctc agaaatctct tccagcgaca ccttccgcgt ttcgatgcca
    39361 aaaatggaga gaaccagcgc acagagcact aacatgacac caagcaccat aaacaccgtg
    39421 atcgacccat aatgtgttaa taatgccgca acgccatagg gcgtgaaaac tgcgacgatc
    39481 cgtccgacgg cattaacgaa acccgaaccg cgcaggcgta aatgcgttgg ccaaagctcc
    39541 gggatataaa ccgctgacgc gaagcaaaca tacatgtata aaaagaagat catcaccaga
    39601 ccatagatta aaatcgccca ctctgtagtc tgaattgaat agatatagcc taacacggca
    39661 ataataataa gtaaggcgga gccaaataat cgacgaggaa aatgatcaat aattaatgcc
    39721 gcaataaata ttcctaccgg agcaccaatc ataataacag cggtcattaa tattgattta
    39781 tcgacatcaa tcccggagtt aacaaatatg gtcggtatcc atacggtaat ggtataaagc
    39841 gaaatgttca ttgcaattaa aacagttatc gcaactaatg tacgtcgtaa catttcccct
    39901 ttaaacagga gccagaaggt acccttgatt accttaacct tgctgttgct ctggttcaaa
    39961 gtaagctggg gtaaacgaat actcttctcc ctttcaattt gctgctctac ttcacgaagt
    40021 tggctttctg caccggcgat ttgccctttc cctgccagcc atcgtggcga ctcgataaag
    40081 tatttacctg agagaaacca ggctaacagt atgccaatac cacccagaag aaacattatt
    40141 cgccaactaa aaaaagcgat aaccaccacg cctatcgccg cagacagcat gggcgaccag
    40201 ttaccaacaa atgagagccg cgcggaccat tttccacgca ccgtcgcggg gataaactcc
    40261 gtaaatgagg catagccaac cataatcagc gcccccattc ctgttcccat caggaagcga
    40321 aagaaaatga gccagtacat atcagggaca aaagcggccc ctgttgcagc aataccgacg
    40381 atgagaagat ttatgcgaaa cgccctgcgc cgcccaaagt agtcaccaat aaacccacca
    40441 gtaagtgagc cgatgaaata accaaacatt aatgccgagg taaaggcggc attgaggaaa
    40501 ttatttgacc atccattgct taccagcttt gctaagacga cattacctga gtaactcaaa
    40561 aaccccgtta ataacagact aaagcttatt ataccaaata tacgataatg aaaccgggcc
    40621 aaaggcaagc ggtccagtct tgcgccaatt tgatcatatt gttccattag gtgcctcgga
    40681 tttgctaaaa aggtagcaat tgcggcaaat tcacttgccg catctttatc atcattttat
    40741 ttcaaggcgc aggaacaaat aatattgaca acgtgtgctt aaattaccac accagctgga
    40801 tattattgtc tttgcaaaat gctatccact ctgcacccgg tgatttatca gttattatgt
    40861 aattaatatg actaaaatca gctaactgga caaaagcttt gcgatcaaac ttagagtgat
    40921 caaccaataa cgcaacttct gtcgcctgac ggatcattat ctttttgatt tcagcttccg
    40981 cttcattaga gtccagcgcg ccgctgttaa tatcaagacc tttacaactc attaccatga
    41041 tatcgacatg gtagcggctg atgatctctt tagtaattct tccttgcagg gaaagtgtat
    41101 ttttgtttag ttcccccccc gttgaaacga ctttaatttc tgactgagcc agaacatgaa
    41161 tcgcttctgc cgagttggtt agcaacgtca ggtcactacg gtcctgtaac aatttgagca
    41221 attccattac ggtactactg gaatcggctg ccatggtggt tttattgtcg ataaagggta
    41281 atgccttgcg tgcaataagc tgcttctctt cataaaacga tgaagcgcgc ttataaaaat
    41341 ggatattctc cgtcaacatc gctgtattta aaacagcacc accataggtt ctggtcaaaa
    41401 agccttcatc ttccagcttc tcaagatcgc ggcgaatggt ttcttcggtt acctgaaaaa
    41461 tcccactcaa atttgagact gtcacctttt tatcgttggc aaccatttgc ttaattgcct
    41521 gaatcctgtc ttttgccgcc acgattacac ccctgtatct ttttacatca cattagcgcg
    41581 attatcgcat aaccgatgtt tactttcaaa ataacctgtt tgaatcacag attttcatca
    41641 cagctttcac agaaacagag gtgaatcgtg ttgagtattt aacgctccag ggcctctgcc
    41701 atttccctca tcaatgttgc gtctgcatcc gataagttga tattcagtgc cgcgacattt
    41761 tcgcgtacct gttccggtgc agtagcccca ctaagaatgg agattaaatc actctgtttt
    41821 aatatccacg ccagtgccag agtgggaatt gtgcactgat aacgagcaca aagtggctgc
    41881 cactgttcaa gcatatcaat cactttcagc atgttttcac gctggaacca gactttattt
    41941 gcccgagcgc cgcccggaac gtaatcacga gtgatggtgc cggtcaacaa tccctgctct
    42001 agcggggaat aaacctgaac cacaatgcca ttatcacgac atagtggcag cagttcgttt
    42061 tccattgccc ggtcgaggat actgtatttc gcctgaataa tatccagttc accatattgc
    42121 agatactcgc ggatatggtc agcatcgacg ttagcagcgc ctatagcgcg aattttccct
    42181 tcagacttta actcattaag cactgcgaca gtttcagcga tcggcgtaaa aaatggcggc
    42241 accgactgcc agtgcgtcat gtagatatcg atgtaatcaa tacccagacg ttgcaagctc
    42301 gctgctacct cttcgcggat agattccggg gaaagttttt tatacaactg ccgatcgcca
    42361 actttgttga ataaacttcc ttttcgttcc cagacaatgc cgcatttggt ttctactaca
    42421 acctgttcac ggggcagttt ttttaacgcc tgaccgacga taacttcact attgccaaag
    42481 ttatatcctg gcgcagtatc aatcagatta atgccacaac gatgggcttc aagaatcgta
    42541 tcaatacata tttgccgatc gagatcgcca ttccatgcag gaccgccgcc aatggcccat
    42601 gtccccaacc ccattcgcga aagcgtaata tccgttgtgc ctaaaggtat ctttttcatt
    42661 tgccttcatt atccttcgta ttcttccagc aattgttcga ccagctttct gttttttacg
    42721 ccggtggtgg caccgacgct tagaaccgag atagccgccg ttgcattggc aaagcgtgcg
    42781 cattcacgca gatttttgcc ttctaacagt gccgcaataa aacctgaagc aaagttatcg
    42841 cccgcgccaa tggtgtcgat ggcggttatt cctgcgaccg ccggcacctt catcgtcatg
    42901 tcaccacgct tgataaagca gccgtcttta cccgttttaa tcaccaccgt ttttacgccg
    42961 cacgcaagaa agcagtcagc aatttcatcc agtgtctctt tcccggtgag taattttgcc
    43021 tcggcaaaat taggaaacag ataatcgaca taactcaatg cttcgcaaat atcatccagc
    43081 gtttcattca accgcggttt gatcatatcg gcacagataa tcatctgccg ggctttagct
    43141 tgcgtaaaaa tttctgttag cgctttacca tccaatagtg gactgttgaa aatactggcc
    43201 agtgataata atttcgcctg agaaaaccgc gcaaaatcaa catcgtcaat attcaatttc
    43261 cacagactgc cattacggtt tgtgacaaac gtccgctcgc catcctccgt caccagtcca
    43321 acgttaatag aggtatctat gctgacgtcc tgcttcaggc tttgaatatc aatattctct
    43381 ttgcggcaat ggtcgagaat aaattgccct gcggcatctt taccaatacg actcattaat
    43441 gctgtgcgat ggcccagacg agaaataatt gttgcttcat ttattgcatc acccccggtg
    43501 gtcattgcga ttctttcaag aggataagaa tccacatcaa agatattttt actgaccggt
    43561 tgcaatggaa tatcaacaat agcggcacct atacaaataa cgtcgagatt atccatatca
    43621 ttattccgct ttgccatcag aaccgaacag tttaattttt tccaatgccc gttcttttac
    43681 agctttacgt acttcacgtt ctaaatgcag gaaaggctga tcctggtttt ctttcactgc
    43741 aaccattgcg gcctgacaga gctccgtatg aatgttgatt ttcgcgatac ccaacgaaat
    43801 tgcagtttta atgtcggcat cactgatccc cgatgcaccg tgcaaaacca gcggaacaga
    43861 aacggcatcg cgtacgcgtt tgacgacctc aaagttcaat tgtggctctg acgtataaac
    43921 cccatgctgg ttgccgatgg cgacagccaa tgaatcacag cccgtacgtt caacaaattc
    43981 agccgcctgg tcaggatcgg tatagtgata gcccgccagc gcttcctcat aaacggtttc
    44041 attaccgaca tgccctaatt ccgcctctac cggaataccc agcggatgga agaaatcgac
    44101 agcctctttg gttaaacgaa tattttcttc gaaatcaaac gcggaagcat cacgcattaa
    44161 tgaattcata ccatgagtcc aggcgttatg aataatctcc atactccgac catgatccca
    44221 atgagttatt accggcaccg ttgctttttg tgccattgat accatcatgt gagagaaatc
    44281 ttcaaatgag gtgttaccga caaaacctgt accaaaagaa ataataaccg gggatttcgc
    44341 ttcttcggct gcgtcgataa cgcccatcaa catttctgca ttccatacgt taaaatgggc
    44401 aattgcataa tgtttatttg tggcatcgtt ttcccaatat ctgatatctg cgagcatgtt
    44461 ctgttctccc gtattaatta atcgctaatt ttaataacgc ctttaataat gtcgcgtttg
    44521 ttgttaactg actcttcaaa tgcctgttgt acatcccgat aatcgtaaat atgcgtcacc
    44581 atcgatttca catcgaatcg ccctgaagaa atagcttcaa tcgtgaccgg ataacgattg
    44641 gcatagcgga ataccgtctg gatagtgact tcgcgattga ttttgaggaa attgattgcc
    44701 gaatcgccgg gtacagtacc aacaatcata attttaccgc cgcgcattac cagataaggt
    44761 gcctgtttaa cggtgaccgc agaacccgct gtttcgaaaa caatatctgc gcccatgtct
    44821 tcggtaaatt gctgacagcg tgcaatagtg tcttcttttg tgccgttaat aaccactgtc
    44881 gcaccaagtt gttccgccat tatcagacgt ttttccagca catcaacgac ggcaatttcc
    44941 gttgcgccca ggcatttaca cgcttgcaac gtcatcaaac caatacaacc tgctcccaga
    45001 ataattatct tcttacccgg tttaacatct gccagcatcg cggcatgcat cccgactgcg
    45061 gcaggctcca ccagcgcccc ttccatcgtg tccatattgt ctggcagttt gtaagtaaag
    45121 ctctccggat gacacagata gtgcgttaat gcgccgcggt agttgggttg tgtcgccata
    45181 aaatcaacgt ccgggcagat gttatatttg ccttccagac agtaacgaca gtgaccgcaa
    45241 ggaacgccag gttcgatatt tacccgatcc cccggtttaa atttgcgcac gcggcttccc
    45301 acagccacaa ccgtcccggc gcattcatga cccaggccaa tttcttgatt tgggtcttta
    45361 ggcggaataa acgggcctga ttcaaaacca tgtacatctg aaccacaaat accgacatat
    45421 tctactttaa tcaaaacttc atcttcttta ggcactggta tttctgctga aataattttc
    45481 attgtgcccg gcacctgcaa tattgctttt gaatttttca ttttgaatcc acccgtatta
    45541 tttattggct actgcatcaa tacttaaact ttcaacggag acacctttgg tttcaattcc
    45601 aatggtggcg atagcaattg cgacaacaat tgaaacagcc cccagaagaa taaagactcc
    45661 cgttacgcca taactactga gcagcactgc aacggcataa ggtgcggcaa taccactgat
    45721 tcgccctacc gcattcgcca gaccggagcc acggagtttg gcctctgtcg gccagatttc
    45781 agggacatac actgccgagg catagcaaac gtacatatag acgaaagtaa tcaggaagaa
    45841 accaatcaac gttatgagca acatactggt ttgcagtgaa tagatatatc cgagcaccgc
    45901 aatcaggatt aatagcccca cacccattgt tttacgcgga attttatcca tcaccagcat
    45961 ggcaataaaa ataccaaatg gcgcgccaaa catactcatg gtatttaaaa caatcgagtc
    46021 ttttaaatta atcccctggg tcatgaatat tgttggcaac cagttaatta gtgtgtactg
    46081 cacaacgttc atggcaatca gcacacaaga gcctaatatc acgcgtttca gtaatactcc
    46141 tgtcagtaag gctgaatacg gcaccgcttg tggcgcttta ccgtcatcag caataaccac
    46201 aggcggcaaa ggtttacccg tctggcgtat gacgccttct tctatactcc gcatgacttt
    46261 ctcggcttct tgataccgtc cgcgcgattc cagccagcgc ggggattcag gaaagtagcg
    46321 ccaggccagc gccgtagcga taagcgacaa tattgcaggg ataagcagtt gtacccgcca
    46381 gttccactct gcactaatca gcggcgtgag tcccatcgca atcaatgaac agagcggata
    46441 tgaccagttg ccaataaagg aaacccgact ggaccacgtt ccgcgatttc taccgggcat
    46501 atattcggtg aaaccagcaa acagtgtaac cagtaaagct cccagcccaa cgcccatcac
    46561 aaaacggcag gcaataagaa aatccatatt cggtgagaaa gcaccgacaa ccatcgaggc
    46621 aatatgaatg gcctcataga ggatgaacgc atttctgcga ccggttttgt cgccaatgat
    46681 gccgcccacc agcgcaccga gaaacattcc ggcggtcgtg attgctgaga atgtggctgt
    46741 ggtggaatta tctgtccagc ccaacgcttt cagctgcgcg aggatcaagc caccaacggc
    46801 attactccag cagacaagca agccaaacgc gacgatggca aacattgacg aatgccagcg
    46861 gcaatccggt aagcgatcca gccgtgcacc acaatgcggt tttgttatct gttccatgcg
    46921 ataacgttcc ttcaggagag tagttattca tcaaagtcgt aagtcatgat cactttgatt
    46981 gcggttttat cgaccatcgc atcaaaccct tcgcgccatt gcgacaggcc gatacgatgc
    47041 gtgatcatcg gtttgacttt gatagcgccg ctggctaata gccggatagc gttacgccat
    47101 gaggtggagt cataggccat atgcccaatg atgcttttat tccaggcggt aatgtcatta
    47161 atcgagaaat caagaggttt gaagcccatt ccaacgcgta ccacttcccc attcgggcgg
    47221 agcatttcga tggcttgttt cagtgcgata ttggcaccgg agcattcaat caccagtccc
    47281 agattatctt tgccacaaat ttgctggcag cgcgccacca catcttcggt agaaccattt
    47341 actactgccg tcgcccccag ttcttttgca accgggaagc ggaccgccac atcttcttgc
    47401 agaccaacga cgacgatatt taccgccccc ataatccgcg ccatttgtac ggaaaacagc
    47461 ccgagtgggc cagtgccgat gacgacaaca tcctgaccag gaaggaattt cgattgctgc
    47521 gcgatggatt tataggcatt acagataggg tcaagtacgg ctgcgtcctc ataatcaaca
    47581 ccatcaggga tttcccacaa cgcatgacga tgaattttga gaatttcacc aggaaccaga
    47641 caatatttgg aaaaaccacc gccccaggta ttgttatcca gaccaaggtt tactttttct
    47701 gtacaacaca gaaaatcacc ctgttcacag gccggacaaa cgccgcaaac gtgaccgctg
    47761 ttatccgaca cgacgcgttg ccccactttc cagtctttga ctttttcacc aacctgcgca
    47821 atacaacctg cgaactcatg gccgcggata gagttaaact catcagaacc gctatcgaca
    47881 ttgtagtgct tcatgtctgc gccgcagatt gccgcggctt taatttcaat cactacgtct
    47941 tccgggccac aaatgggttg tgggacatca atcatcttgt agccgccaaa ggccttgcca
    48001 aaccgagcca gtgctttcat tgcgtcatcc tctgtgtttt gttttgctta gatggccatc
    48061 atacttaccc acatgatttt tgaggtcaac attaaatctg taaaacacag ataatgatct
    48121 gcgttttaca acttagatca caattacgca gaaaatggct gggtctgtta caggttggtg
    48181 gaaggcggga cgcaaaaaga gcaaattcga gagggattac cggccaaata ggtctgagac
    48241 ttaagttcag gagaagtcat tcaggcgtga acgcgactgc aagagatcgg actgggactt
    48301 agccaggtcg ggtcgagata attgagacga gtcgtggggt cattgttggc tatccccttc
    48361 atagccgcgg caaaaagggg ataaaccact tacttaaaca tagcaatagg ctttgctgag
    48421 ataccaaaat cttcttttaa ctgctgtttg ctcttcatga ccatctgacc atttgtgtca
    48481 attgtcatat gctgtgcttc ggtattatgg cgggcttgcc acagcatcac cagttgcagg
    48541 caattctctt tttgttcctc ggtcaacgca acgccatcag gccatttccc cagttcaacg
    48601 gcggtcgaca aacgctggta tacctcaggc gtcatgctgt tgataatgtc atcaagattc
    48661 ataatttttc cgctcctgtg gaataagttg ctgaatcgtt tttttcaacc gttgatttct
    48721 tcgccgtttt cgccatcggt aaagcgtaaa gaggcagagt taacacaata acgttcgccc
    48781 gttggctgcg gcccgtcagg gaagacatgc cccagatggg catcacagtt accgcaacga
    48841 atttctatgc gctgcattcc atgtgacaag tctttgatat aacgaatgga ttcttcactc
    48901 accggttcgt agaaactggg ccaaccacag ccggaatcat acttggtttg ggaatgaaac
    48961 agcggggcat cgcagatcaa acagtgatat acgccgtcac gcttgttatg cagtaaacga
    49021 cccgtaaatg gcggctctgt cccatgattc tgcgtcacgt aaaactgcat ctcggacaaa
    49081 ttttttttca gttcttctgc cgaaggttta ttagccattt gctcacatct cactttaatc
    49141 gtgctcacat tacgtgactg attctaacaa aacattaaca ctaactggca aaattttgtc
    49201 ctaaacttga tctcgacgaa atggctgcac ctaaatcgtg atgaaaatca catttttatc
    49261 gtaattgccc tttaaaattc ggggcgccga ccccatgtgg tttcaagccc aaaggaagag
    49321 tgaggcgagt cagtcgcgta atgcttaggc acaggattga tttgtcgcaa tgattgacac
    49381 gattccgctt gacgctgcgt aaggtttttg taattttaca ggcaaccttt tattcactaa
    49441 caaatagctg gtggaatata tgactatcaa agtaggtatc aacggttttg gccgtatcgg
    49501 tcgcattgtt ttccgtgctg ctcagaaacg ttctgacatc gagatcgttg caatcaacga
    49561 cctgttagac gctgattaca tggcatacat gctgaaatat gactccactc acggccgttt
    49621 cgacggtacc gttgaagtga aagacggtca tctgatcgtt aacggtaaaa aaatccgtgt
    49681 taccgctgaa cgtgatccgg ctaacctgaa atgggacgaa gttggtgttg acgttgtcgc
    49741 tgaagcaact ggtctgttcc tgactgacga aactgctcgt aaacacatca ccgctggtgc
    49801 gaagaaagtg gttatgactg gtccgtctaa agacaacact ccgatgttcg ttaaaggcgc
    49861 taacttcgac aaatatgctg gccaggacat cgtttccaac gcttcctgca ccaccaactg
    49921 cctggctccg ctggctaaag ttatcaacga taacttcggc atcatcgaag gtctgatgac
    49981 caccgttcac gctactaccg ctactcagaa aaccgttgat ggcccgtctc acaaagactg
    50041 gcgcggcggc cgcggcgctt cccagaacat catcccgtcc tctaccggtg ctgctaaagc
    50101 tgtaggtaaa gtactgccag aactgaatgg caaactgact ggtatggcgt tccgcgttcc
    50161 gaccccgaac gtatccgtag ttgacctgac cgttcgtctg gaaaaagctg caacttacga
    50221 gcagatcaaa gctgccgtta aagctgctgc tgaaggcgaa atgaaaggcg ttctgggcta
    50281 caccgaagat gacgtagtat ctaccgattt caacggcgaa gtttgcactt ccgtgttcga
    50341 tgctaaagct ggtatcgctc tgaacgacaa cttcgtgaaa ctggtatcct ggtacgacaa
    50401 cgaaaccggt tactccaaca aagttctgga cctgatcgct cacatctcca aataagttga
    50461 gatgacactg tgatctaaaa agagcgactt cggtcgctct tttttttacc tgataaaatg
    50521 aagttaaagg actgcgtcat gattaagaaa atttttgccc ttccggtcat cgaacaaatc
    50581 tcccctgtcc tctcccgtcg taaactggat gaactggacc tcattgtggt cgatcatccc
    50641 caggtaaaag cctcttttgc attacagggc gcacaccttc tctcgtggaa acctgcgggt
    50701 gaagaagaag ttctgtggtt gagcaacaac acaccgttca aaaatggcgt cgctattcgc
    50761 ggtggcgtac cggtttgctg gccgtggttt ggtccggcgg cacaacaagg tctgcctgcg
    50821 cacggttttg cccgcaacct gccgtggacg ctgaaatcac atcgtgaaga tgctgatggc
    50881 gtagcgctga cttttgaatt gacacaaagc gaagagacga aaaaattctg gccgcacgac
    50941 tttacgctgt tagcgcattt ccgcgtgggt aaaacctgtg aaatcgatct tgaatcacat
    51001 ggcgaatttg aaaccacctc tgccctgcat acctacttta acgtgggtga tatcgctaag
    51061 gtaagcgtca gtgggctggg cgatcgcttc atggataaag taaatgacgc gaaagaagat
    51121 gtactgaccg acggtattca gaccttccct gaccgtaccg atcgcgtgta tctgaatccg
    51181 caagattgca gcgtgattaa tgatgaagcg ctgaatcgta ttatcgccgt aggccaccag
    51241 catcatctga acgttgtcgg ctggaacccg ggaccggcgc tttcagttag catgggcgac
    51301 atgccggatg atggctacaa aacatttgtt tgtgtagaaa cggcttacgc ttcagaaacg
    51361 caaaaagtga ccaaagagaa acctgcacat ctggcgcaat ccattcgcgt tgcgaaacgt
    51421 taatttgcgt taatgttgtg tgccgggtgc gatgcatccg gcacgtatca acatcacacc
    51481 atatccagcg cagtttttcc ttttggtgcc ggatacgctt tatccagcat ggttaattcc
    51541 gctgacgaaa gttcgacctc aagcacagcc gcattctgtt ggacatgggc aatcgtggcc
    51601 gcttttggaa tcgccatcac accctgatgg ctgattaccc acgccaacaa tacttgtgcc
    51661 gcgctgatat tgtgagcatg tgcaatttcg ttgactaccg cgttttttaa cagtccattg
    51721 cgcaaccgcc cggcctgggc taatggactg taagccatca ccggcatctg ctgttgctgg
    51781 caccagggga gtagatcgta ctcaatccct cgtgaaccga gatggtaaag cacctgatta
    51841 gtggcacact gatttccccc cggcagctgc cagagttcct gcatatcagc atgatcaagg
    51901 ttagaaacgc cccagcggcg gatttttccc tgggcgatca atttttccat cgctgccaca
    51961 gtctcttcaa aagcgaaact gccagaccag tgtaataagt agagatcgag ataatcagta
    52021 ttgagacggc gtaaactggc ttcgcatgca tttatcgctt tttgcccgcc agcattccac
    52081 ggatagactt tagagacgag aaagacgtta tcgcgcagac cggttaatgc ttccccaacc
    52141 accttttcgg caccgccatc ggcatacatt tcggcggtat caatgagggt taaaccgagc
    52201 tcaatgcccg cgcgtagtgc agcaacttct gttttgcgct gactggcatc ttcgcccata
    52261 taccatgttc cctgccctat ggctggcagt gagacatcgc cactaaattg aatcattttt
    52321 tgttgcattg tttcctccca ggtattgcac caccgtaatg caaaacaggg cgtgacgccc
    52381 tgtttttatg cacaaaatgc cctggaaaga tgcattatca gaatttgtag gtgatcccgg
    52441 tagaaatcag gccagtccag gatttatcca ccatcgggct gtcagtaact tcatcagaca
    52501 gacgggtata gcgcgcggta ccgtaaacac tccagtcgcc gaggaagttg tagctggcgc
    52561 tcagctccag gtaagggctc cagctgtcgt tcgggttata gccacgcaga ccgctgcgag
    52621 cggactcttt gcgcgatacg ccataatagt attcgttctg gttttcgctg ttccactgca
    52681 caccgatacc cggggtcacg gtcaggccgc cgttggtgta acgatacaac caggccatat
    52741 cccagacgat gccgttgctg ttatccaggg tatcgccagc caggatggta cgcaggtaac
    52801 cgtactgggt aaagtgagca taagacagac cagccatcat ggtgctctta cggtcatcca
    52861 ggtgacgcat ttggtgatcg ccactgtctt tcgctttgaa gtaaagcggc gaccaataag
    52921 cggtaattga aagtttgtcc gttgcgtcat tccacaggta gtaaccacca cctaagccac
    52981 ggaaccagaa gttatcgcct tcatagttga ttaccggtac tgggtaaaca tcggtatcgt
    53041 aatctttata tgggtgttca acgacaccta cgcctgcgcc cagggaaaat ttaccttcag
    53101 cgtgcgctac gcctgcagac gttgcgataa gcactccaag tgccagaagt ttgagtttgg
    53161 tcacaattaa tcattcctta aacaaatgtt tagcgggcga caaagtttac ccgtcaatac
    53221 atcgaaaccc aaccttttta cgttttcatt ttttaaagta actgtttaat tttcctgacg
    53281 cgg
//
LOCUS       DAZFAH010000011        50558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFAH010000011 DAZFAH010000000
VERSION     DAZFAH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.011"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            118..2652
                     /gene="mrcB"
                     /locus_tag="ACHJWB_000613"
     CDS             118..2652
                     /gene="mrcB"
                     /locus_tag="ACHJWB_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4312294.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRSKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPALQEQKDSDGVAGWIKDMFGSN"
     gene            2872..5115
                     /gene="fhuA"
                     /locus_tag="ACHJWB_000614"
     CDS             2872..5115
                     /gene="fhuA"
                     /locus_tag="ACHJWB_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4312295.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5166..5963
                     /gene="fhuC"
                     /locus_tag="ACHJWB_000615"
     CDS             5166..5963
                     /gene="fhuC"
                     /locus_tag="ACHJWB_000615"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4312296.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5963..6853
                     /gene="fhuD"
                     /locus_tag="ACHJWB_000616"
     CDS             5963..6853
                     /gene="fhuD"
                     /locus_tag="ACHJWB_000616"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4312297.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAIPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6850..8832
                     /gene="fhuB"
                     /locus_tag="ACHJWB_000617"
     CDS             6850..8832
                     /gene="fhuB"
                     /locus_tag="ACHJWB_000617"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4312298.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGMLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8867..10147)
                     /gene="hemL"
                     /locus_tag="ACHJWB_000618"
     CDS             complement(8867..10147)
                     /gene="hemL"
                     /locus_tag="ACHJWB_000618"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4312299.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10313..10360
                     /gene="yadX"
                     /locus_tag="ACHJWB_000619"
     CDS             10313..10360
                     /gene="yadX"
                     /locus_tag="ACHJWB_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4312300.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10372..11793
                     /gene="clcA"
                     /locus_tag="ACHJWB_000620"
     CDS             10372..11793
                     /gene="clcA"
                     /locus_tag="ACHJWB_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4312301.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11817..11882
                     /gene="yadW"
                     /locus_tag="ACHJWB_000621"
     CDS             11817..11882
                     /gene="yadW"
                     /locus_tag="ACHJWB_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4312302.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11875..12219
                     /gene="erpA"
                     /locus_tag="ACHJWB_000622"
     CDS             11875..12219
                     /gene="erpA"
                     /locus_tag="ACHJWB_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4312303.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12266..12889)
                     /gene="yadS"
                     /locus_tag="ACHJWB_000623"
     CDS             complement(12266..12889)
                     /gene="yadS"
                     /locus_tag="ACHJWB_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4312304.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12927..13727)
                     /gene="btuF"
                     /locus_tag="ACHJWB_000624"
     CDS             complement(12927..13727)
                     /gene="btuF"
                     /locus_tag="ACHJWB_000624"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4312305.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13720..14418)
                     /gene="mtnN"
                     /locus_tag="ACHJWB_000625"
     CDS             complement(13720..14418)
                     /gene="mtnN"
                     /locus_tag="ACHJWB_000625"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4312306.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELKLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14502..16019
                     /gene="dgt"
                     /locus_tag="ACHJWB_000626"
     CDS             14502..16019
                     /gene="dgt"
                     /locus_tag="ACHJWB_000626"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4312307.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFI
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFAELVEKERVKRFPIEMRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16149..17573
                     /gene="degP"
                     /locus_tag="ACHJWB_000627"
     CDS             16149..17573
                     /gene="degP"
                     /locus_tag="ACHJWB_000627"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4312308.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17728..18885
                     /gene="cdaR"
                     /locus_tag="ACHJWB_000628"
     CDS             17728..18885
                     /gene="cdaR"
                     /locus_tag="ACHJWB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4312309.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18974..19360)
                     /gene="yaeH"
                     /locus_tag="ACHJWB_000629"
     CDS             complement(18974..19360)
                     /gene="yaeH"
                     /locus_tag="ACHJWB_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4312310.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="ACHJWB_000630"
     CDS             complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="ACHJWB_000630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4312311.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="ACHJWB_000631"
     CDS             complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="ACHJWB_000631"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4312312.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23264..24058)
                     /gene="map"
                     /locus_tag="ACHJWB_000632"
     CDS             complement(23264..24058)
                     /gene="map"
                     /locus_tag="ACHJWB_000632"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4312313.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24426..25151
                     /gene="rpsB"
                     /locus_tag="ACHJWB_000633"
     CDS             24426..25151
                     /gene="rpsB"
                     /locus_tag="ACHJWB_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4312314.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25409..26260
                     /gene="tsf"
                     /locus_tag="ACHJWB_000634"
     CDS             25409..26260
                     /gene="tsf"
                     /locus_tag="ACHJWB_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4312315.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26407..27132
                     /gene="pyrH"
                     /locus_tag="ACHJWB_000635"
     CDS             26407..27132
                     /gene="pyrH"
                     /locus_tag="ACHJWB_000635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4312316.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27424..27981
                     /gene="frr"
                     /locus_tag="ACHJWB_000636"
     CDS             27424..27981
                     /gene="frr"
                     /locus_tag="ACHJWB_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4312317.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28073..29269
                     /gene="ispC"
                     /locus_tag="ACHJWB_000637"
     CDS             28073..29269
                     /gene="ispC"
                     /locus_tag="ACHJWB_000637"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4312318.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPENFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29458..30216
                     /gene="ispU"
                     /locus_tag="ACHJWB_000638"
                     /gene_synonym="uppS"
     CDS             29458..30216
                     /gene="ispU"
                     /locus_tag="ACHJWB_000638"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4312319.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30229..31086
                     /gene="cdsA"
                     /locus_tag="ACHJWB_000639"
     CDS             30229..31086
                     /gene="cdsA"
                     /locus_tag="ACHJWB_000639"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4312320.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31098..32450
                     /gene="rseP"
                     /locus_tag="ACHJWB_000640"
     CDS             31098..32450
                     /gene="rseP"
                     /locus_tag="ACHJWB_000640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4312321.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32480..34912
                     /gene="bamA"
                     /locus_tag="ACHJWB_000641"
     CDS             32480..34912
                     /gene="bamA"
                     /locus_tag="ACHJWB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4312322.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIELGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35034..35519
                     /gene="skp"
                     /locus_tag="ACHJWB_000642"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35034..35519
                     /gene="skp"
                     /locus_tag="ACHJWB_000642"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4312323.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35523..36548
                     /gene="lpxD"
                     /locus_tag="ACHJWB_000643"
     CDS             35523..36548
                     /gene="lpxD"
                     /locus_tag="ACHJWB_000643"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4312324.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36653..37108
                     /gene="fabZ"
                     /locus_tag="ACHJWB_000644"
     CDS             36653..37108
                     /gene="fabZ"
                     /locus_tag="ACHJWB_000644"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4312325.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37112..37900
                     /gene="lpxA"
                     /locus_tag="ACHJWB_000645"
     CDS             37112..37900
                     /gene="lpxA"
                     /locus_tag="ACHJWB_000645"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4312326.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37900..39048
                     /gene="lpxB"
                     /locus_tag="ACHJWB_000646"
     CDS             37900..39048
                     /gene="lpxB"
                     /locus_tag="ACHJWB_000646"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4312327.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39045..39641
                     /gene="rnhB"
                     /locus_tag="ACHJWB_000647"
     CDS             39045..39641
                     /gene="rnhB"
                     /locus_tag="ACHJWB_000647"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4312328.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39678..43160
                     /gene="dnaE"
                     /locus_tag="ACHJWB_000648"
     CDS             39678..43160
                     /gene="dnaE"
                     /locus_tag="ACHJWB_000648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4312329.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43173..44132
                     /gene="accA"
                     /locus_tag="ACHJWB_000649"
     CDS             43173..44132
                     /gene="accA"
                     /locus_tag="ACHJWB_000649"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4312330.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44231..46372
                     /gene="ldcC"
                     /locus_tag="ACHJWB_000650"
     CDS             44231..46372
                     /gene="ldcC"
                     /locus_tag="ACHJWB_000650"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4312331.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46429..46818
                     /gene="yaeR"
                     /locus_tag="ACHJWB_000651"
     CDS             46429..46818
                     /gene="yaeR"
                     /locus_tag="ACHJWB_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4312332.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46883..48181
                     /gene="tilS"
                     /locus_tag="ACHJWB_000652"
     CDS             46883..48181
                     /gene="tilS"
                     /locus_tag="ACHJWB_000652"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4312333.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENLGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGALQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGNIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48230..48490)
                     /gene="rof"
                     /locus_tag="ACHJWB_000653"
     CDS             complement(48230..48490)
                     /gene="rof"
                     /locus_tag="ACHJWB_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4312334.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48477..48677)
                     /gene="yaeP"
                     /locus_tag="ACHJWB_000654"
     CDS             complement(48477..48677)
                     /gene="yaeP"
                     /locus_tag="ACHJWB_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4312335.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48843..49388
                     /gene="yaeQ"
                     /locus_tag="ACHJWB_000655"
     CDS             48843..49388
                     /gene="yaeQ"
                     /locus_tag="ACHJWB_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4312336.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTCNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49385..49807
                     /gene="arfB"
                     /locus_tag="ACHJWB_000656"
     CDS             49385..49807
                     /gene="arfB"
                     /locus_tag="ACHJWB_000656"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4312337.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49821..50531
                     /gene="nlpE"
                     /locus_tag="ACHJWB_000657"
                     /gene_synonym="cutF"
     CDS             49821..50531
                     /gene="nlpE"
                     /locus_tag="ACHJWB_000657"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4312338.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADVA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        11924 a        12438 c        14067 g        12129 t
ORIGIN      
        1 tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc ttcttctgtc
       61 ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg
      121 gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa
      181 aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga tgactatgag
      241 gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa gcctcgtagc
      301 aaacgcggct ggctatggct actgctaaaa ctggctatcg tttttgccgt gctgatcgcc
      361 atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa
      421 ctgcctgcgg cagtttatgg ccgaatggtc aatcttgagc cagatatgac catcagcaag
      481 aacgagatgg tgaagctact ggaggcgact cagtatcgtc aggtgtcgaa aatgacccgt
      541 cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc gtttgatttc
      601 ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggtga tcatctggcg
      661 acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga tccgcgcctg
      721 atcaccatga tctcctcgcc aaacggtgag cagcgtttgt ttgtgccgcg cagtggtttc
      781 cctgatttgc tggtggatac tttgctggcg acagaagacc gtcattttta cgagcatgac
      841 ggcatcagcc tctactccat cggacgtgcg gtgctggcaa acctgaccgc cggacgcacg
      901 gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa acctgttcct ctccagtgag
      961 cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga cgcgcgttac
     1021 agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca gagcggcgac
     1081 aacgaaatcc gcggcttccc gctggcgagc ttgtattact ttggtcgccc ggtagaagag
     1141 ctaagcctcg accagcaggc gctgttagtc ggcatggtga aaggggcgtc catctacaac
     1201 ccgtggcgta acccgaaact ggcgctggag cgacgtaatc tggtgctgcg tctgctgcaa
     1261 cagcaacaga ttattgatca agaactctat gacatgttga gtgcacgtcc gctgggggtt
     1321 cagccgcgcg gtggggtaat ctctccacag ccagccttta tgcaactggt gcgtcaggag
     1381 ctgcaggcaa aactgggcga taaggtaaaa gatctctccg gcgtgaagat cttcactacc
     1441 tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat tccggcactg
     1501 aagaaacagc gtaagctgag cgatctggaa accgcgattg tggtcgtcga ccgctttagt
     1561 ggtgaagttc gtgcgatggt cggaggttct gagccgcagt ttgcgggcta caaccgtgcg
     1621 atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct gactgcctta
     1681 agccagccga aaatctatcg cctgaacacg tggattgcgg atgcgccaat cgcgctgcgt
     1741 cagccgaatg gccaggtctg gtcaccgcag aacgatgatc gtcgttatag cgaaagtggc
     1801 agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt aaatctgggg
     1861 atggcactgg ggctgcctgc ggttacggag acctggatca aactgggcgt gccgaaagat
     1921 cagctgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc aatcgaagtg
     1981 gcgcaggcat tccagaccat cgccagcggc ggtaaccgtg caccgctttc tgcgctgcgt
     2041 tcggtaatcg cggaagatgg caaagtgctg tatcagagct tcccgcaggc ggaacgcgct
     2101 gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt acaacgcggt
     2161 acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa aacagggact
     2221 actaacaata acgtagatac ctggtttgcg ggcattgacg gcagcacggt gaccatcacc
     2281 tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg ggcaatgtcg
     2341 atttatcagc gttatctggc taaccagacg ccaacaccgc tgaatcttgt tccgccagaa
     2401 gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg tggcatgcgt
     2461 gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagtga gatgcagcag
     2521 cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc gcaacagcaa
     2581 cctgctctgc aagagcagaa agacagcgac ggtgtagccg gttggattaa agatatgttt
     2641 ggtagtaatt aacgtctaag cgtgaaatac cggatggcga gttgccatcc ggtaaaataa
     2701 catcccaccc aaagtattaa ccctttcttt tcatctggtt gtttattaac ccttcaggaa
     2761 cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc ttatctttat
     2821 aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc aatggcgcgt
     2881 tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt agccacagcg
     2941 gttagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga cactatcacc
     3001 gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg
     3061 cgacagtcag ctaccggcac taaaaccgat acgccgattc aaaaagtgcc acagtctatt
     3121 tctgttgtga ccgccgaaga gatggcgctg catcagccga agtcggtaaa agaagcgctt
     3181 agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta tgaccacctg
     3241 atcattcgcg gttttgcggc agaaggccaa agccagaata actatctgaa tggcctgaag
     3301 ttgcagggca acttctataa cgatgcggtc attgatccgt atatgctgga acgcgctgaa
     3361 attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg tctgttgaat
     3421 atggtcagca agcgtccgac caccgaaccg ctcaaagaag ttcagtttaa agccggtact
     3481 gacagcctgt tccagactgg ttttgacttt agcgatgcgc tggatgatga cggcgtttac
     3541 tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg gtcagaagag
     3601 cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac caatttcacc
     3661 ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt gccgaaagag
     3721 ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa tgaaggggcg
     3781 aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga tcacgaattt
     3841 aacgacacct ttactgtgcg tcagaacctg cgctttgctg aaaacaaaac ctcgcaaaac
     3901 agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa acagtgtgcg
     3961 gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt tgatgatgag
     4021 aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac tggcgatatc
     4081 gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat caacgcctgg
     4141 tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa taccgatttc
     4201 gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa taaacagaaa
     4261 caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt caccctgggc
     4321 ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgccgggac gaccgataaa
     4381 cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga taatggcgta
     4441 acaccttact ttagctatag cgaatcgttt gaaccttctt cgcaagttgg gaaggatggt
     4501 aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata tgtaccggaa
     4561 gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa caacctgatg
     4621 gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc acgtggcgta
     4681 gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc ttatacttac
     4741 accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc acaggtgcca
     4801 aaacacatgg cttcactgtg ggctgactat accttctttg acggtccgct ttcaggtctg
     4861 acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc taactccttt
     4921 aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc gcgagtcggc
     4981 atggctggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga atacgtcgcc
     5041 agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca
     5101 accttccgtt tctaatttct cttttggggc acggatttcc gtgcccattt cacaagttgg
     5161 ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt aatatctcct
     5221 ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct gccgggaaag
     5281 tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa atgcttggcc
     5341 gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc ccagccgctg gaaagctgga
     5401 gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct ccggcagaag
     5461 ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc gcgttggggc
     5521 gctttggggc ggcagatcgc gaaaaagtcg aggaagctat ctcgctggtt ggcttaaaac
     5581 cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcaacgg gcgtggatcg
     5641 ccatgctggt ggcgcaggat agctgttgtc tgttgctcga cgaaccgact tcggcgctgg
     5701 atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag gagcgtggcc
     5761 tgacggtcat tgccgtgttg cacgatatca atatggcggc acgctactgt gattatctgg
     5821 tcgccctgcg cggtggtgaa atgattgctc agggaacgcc tgcggaaatt atgcgcggcg
     5881 aaactctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg gcgggtgctg
     5941 cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt
     6001 aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg cggcggctat
     6061 tgaccccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc tggcgctcgg
     6121 catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca gcgaaccacc
     6181 attgccggac tcagtgattg acgtcggttt gcgtacagaa cctaaccttg aactgctgac
     6241 cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcac cagaaatgct
     6301 ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac ggcaaacagc cgttggcgat
     6361 ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac ctgcaaagcg cagcagaaac
     6421 gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg tgaagcgtgg
     6481 tgcacgcccg ttattgctga cgacgcttat cgatccgcgc catatgctgg tcttcggtcc
     6541 aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct ggcaagggga
     6601 aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcggcgt ataaagacgt
     6661 tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa tggcaacccc
     6721 gctgtggcag gccataccgt ttgtccgcgc cggacgcttt cagcgcgtac ctgcagtctg
     6781 gttttatggt gcgacgctct cggcaatgca ctttgtgcgc gttctggata acgccatcgg
     6841 aggtaaagcg tgagtaaacg aattgcgctt tttccggcgt tattgctggc gctgttagtg
     6901 attgtcgcta cggcgctcac ctggatgaac ttctcgcagg cgctgccgcg tagccagtgg
     6961 gcgcaggctg cctggtcgcc ggatattgac gtcatcgagc agatgatttt tcactacagc
     7021 ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcgg gcctggggct ggtgggcgtg
     7081 ctgtttcagc aagtgctgcg taacccgctg gcggaaccga cgacgttggg cgttgcaaca
     7141 ggcgcgcaac tggggattac cgtcactacg ctctgggcga tcccaggtgc tatggcgagc
     7201 cagtttgctg cgctggcagg ggcttgcgtt gttggcttaa ttgtctttgg cgtcgcgtgg
     7261 gggaaacggc tttcgccggt aacgcttatt ctcgcggggc tggtagtgag cctttattgt
     7321 ggggcaatca atcagttact ggttatcttc catcatgacc aactgcaaag catgttcctg
     7381 tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt atggccgcag
     7441 ttgctgggcg gcgtgatgct gacgttattg ctacttcgcc cgttaaccct gatggggctt
     7501 gatgatggcg tggcgcgcaa tctcgggctg gccttatcgc ttgcgcgtct ggctgcgctg
     7561 tcgctggcga tagtcatcag tgcgctgctg gtgaacgcag tggggattat cggctttatc
     7621 gggttgttcg cgccactgct ggcaaaaatg ctgggggcgc ggcgtttgtt gccacggttg
     7681 atgctggcgt cgctgattgg tgcgttaatt ctgtggcttt ctgatcaaat catcctctgg
     7741 ctgactcgcg tgtggatgga agtttccacc ggttcggtca ctgcgttgat cggtgcgccg
     7801 ctgctactgt ggctgctgcc gcgtttacgc agcattagcg cgccggatat gaaggttaac
     7861 gatcgtgtcg cgactgaacg ccaacatgtg ctggcgtttg ccctcgcggg cggcatgctg
     7921 ttgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg
     7981 gcgagcgggg cgttgctcga ggatttaatg ccctggcgct ggccgcgaat tatggcggcg
     8041 ctgtttgcgg gcgtcatgct ggcggtagcg ggctgtatta ttcagcgact gaccggcaac
     8101 ccgatggcaa gcccggaagt gctggggatt agctccggcg cggcgtttgg tgtggtgtta
     8161 atgctgtttc tggtaccggg taatgccttt ggctggctgt tacccgcagg cagtctcggg
     8221 gcggcggtga cgctgttgat cattatgatc gccgccggac gaggtggatt ttccccacac
     8281 cgcatgttac tggcggggat ggcgttaagt accgcgttca ccatgctttt gatgatgttg
     8341 caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct ggatttccgg ttcgacctac
     8401 aacgcgaccg atgcgcaggt ctggcgcacc ggaattgtga tggtgatttt gctggcgatt
     8461 accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac cgcccgagcc
     8521 gtaggaatgg cgctaacgcc gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg
     8581 gcgaccgcga cgatgactat tggaccgttg agttttgttg gtttaatggc accgcatatt
     8641 gcgcggatga tgggctttcg acggacgatg ccgcacatcg taatttcggc gctggtgggt
     8701 ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tgctgtttcc attccagatc
     8761 ccggcggggc tgctgtcaac ctttatcggc gcgccatatt ttatctattt gttgagaaag
     8821 cagagccgtt aattgaatgc ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa
     8881 acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc gccacggaca
     8941 taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcatccagc atcatatgga
     9001 agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac gtcacggact
     9061 cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc agcggaattc
     9121 cggcttcttc tgccgcttcc agcagacctt ctgccagacg tgatgtcagc tcatccaacg
     9181 tttcgtgaac gcccggctgc gcgacttcat tcagacaggc gaaaccggct gccatcgcaa
     9241 ttgggttacc ggaaagcgta cccgcctgat agaccggacc cgtcggggcc agcgcatcca
     9301 ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg atttttccga
     9361 ggcaggttaa atctggcact acgccgtaat aatcctgtgc accagccagt gccacgcgga
     9421 agccagtcat tacttcatcg atgatcagca atgcgccaaa ttcgtcgcac agcgcgcgca
     9481 gacctggcag gaactctggc agcggcggaa cacagttcat attgcctgcc accggctcaa
     9541 cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca gaagccagat
     9601 cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc ggcgagtttg
     9661 gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt
     9721 ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg gccaggcgga
     9781 tggcgctcat ggtcgcttca gtgccggagt tcaccatgcg caccatatcc atggtcggga
     9841 ccagttcggt caccagttgc gccattttca cttccatttc ggttggtgca ccaaagctta
     9901 aaccacgctc ggcggcttca atcacggcat tgcggattgc cggatggtta tggcccagca
     9961 ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca tcgtacagat
    10021 aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa
    10081 caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga ttttcagact
    10141 tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct atgttattca
    10201 taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt
    10261 agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt tcatggaacg
    10321 tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg gatgaaaact
    10381 gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca actgattcgc
    10441 caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc agtcgtcggc
    10501 acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa
    10561 cgtatggggg tgctggtaca tactgctgat aattatccgc tactgttaac cgtcgctttt
    10621 ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata cgcgcccgaa
    10681 gcagggggat cggggatccc ggaaattgaa ggggcgctgg aagatcaacg tcctgttcgc
    10741 tggtggcgtg tattgccggt gaaattcttt ggcgggctgg ggacactcgg cggaggcatg
    10801 gtgctagggc gcgaaggacc aaccgtgcag atcggcggca atatcggtcg catggtgctt
    10861 gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac cggtgctgct
    10921 gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat tatcgaagag
    10981 atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat tggtgtcatt
    11041 atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga cgtcggtaaa
    11101 ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat tatttttggc
    11161 attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct gcaccgtgtg
    11221 cacggcggca atattaccaa atgggtactg atgggtggtg cgattggcgg tctgtgtgga
    11281 ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct
    11341 accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc
    11401 accttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat gctggcgctg
    11461 ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agctgtttcc gcaatatcac
    11521 ctggaggcgg ggacgtttgc tattgccgga atgggggcat tactggcggc atctattcgc
    11581 gcgccgttaa cggggatcat tctggttctg gagatgaccg ataactacca gctcattttg
    11641 ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg
    11701 ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca actggcgcga
    11761 agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat tagataatgg
    11821 cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca aaatatgagt
    11881 gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt taaaagcctg
    11941 atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc
    12001 agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga tatgaccatc
    12061 gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt
    12121 tccgttgatt ataccgaagg tctggaaggt tctcgtttca tcgtgaccaa cccgaacgcg
    12181 aaaagcacct gcggttgcgg ttcttccttt agtatctaat ctgttgttca tgtgccggat
    12241 aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca aacgtcggta
    12301 gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc atgcccatca
    12361 tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg acaataccgc
    12421 cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt tcgcgggcca
    12481 gtacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa accgcgatta
    12541 acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac accgccagac
    12601 caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg cgcaccagca
    12661 cgatggtcag catgctggtg accattgcaa cgaccagatc ggtgggatct ttcacccaaa
    12721 ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gccgcctact gcggtaacca
    12781 cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccgg
    12841 agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc
    12901 cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt acagagctgt
    12961 tgtgcagcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata
    13021 acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg
    13081 cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct aacttgcggc
    13141 cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttc
    13201 tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt
    13261 tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag cagggattgc
    13321 gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg taacgcattg
    13381 gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc
    13441 tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc
    13501 gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct
    13561 tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca
    13621 agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc
    13681 gcaagaaaag acagggcgac cagcgccctg aacagtgact tagccatgtg caagtttctg
    13741 caccagtgac tcaaccatca ggctagactg tttagcggca acagccagga actcatcgaa
    13801 gctaagatga gactgttgat cggccacgtc ggagatggcg cgtacgacaa caaacgggac
    13861 gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg
    13921 tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct
    13981 aacaatcagg ccacgtacag cgttaagttt cagttcggca atgcaggcct cagcggcagc
    14041 gatcagttta tcgtcagctt taaagcctgc cggacagcct ggtaactgac cgtattcata
    14101 accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc
    14161 cactttcaac gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt
    14221 gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga tgcccgattt
    14281 cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac cgccgagact
    14341 gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc
    14401 aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc atagtttacc
    14461 atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc
    14521 gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg
    14581 agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc
    14641 gtctgcaaca aaagacccag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc
    14701 ttacccactc gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa attttaagcc
    14761 gtctgaaaga gcttaaatta ctggaagcat acggcctgga tgaactgacc ggcccctttg
    14821 aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg tttggtcatt
    14881 ttggcgaagc ggcgataaat gattggtttc gccagcgttt gcacccggaa gatgccgaaa
    14941 gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg gacggggaag
    15001 aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt atgtcatttt gaggggaatg
    15061 cacaaggcat tcgtctggtg catacattga tgaggatgaa tctcacctgg gcacaggttg
    15121 gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc
    15181 actatttaat gaaaaagccg ggttattatc tttctgaaga agcctatatt gcccggttgc
    15241 gtaaagaact taatttggcg ctttacagtc gttttccatt aacgtggatt atggaagctg
    15301 ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa agaatattta
    15361 ccgttgagca gctttatcat catttgcacg aggcgtgggg tcagcatgag aaaggttcgc
    15421 tcttttcgct agtggttgaa aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca
    15481 gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa ctggtaccat
    15541 acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg tttaatcatg
    15601 cattattgga agatgccagc gaatgcagcg atcttcttaa actatataaa aatgtcgctg
    15661 taaaacatgt gtttatccat ccagatgtcg agcagcttga attgcagggc tatcgggtca
    15721 ttagcggatt attagagatt tatcgtcctt tattaagcct gtcgttatcc gactttgctg
    15781 aacttgtaga aaaagaacgg gtgaaacgtt tccctattga aatgcgctta ttccacaaac
    15841 tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg tcagattctc
    15901 ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat tatatcagcg
    15961 gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta gaacaataac
    16021 caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt agttcggaac
    16081 ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt taatcgagac
    16141 tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt ttaggtttgg
    16201 cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca gcccagcaga
    16261 tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc attaacgtag
    16321 aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg
    16381 atgattctcc gttctgccag gaaggttcac cgttccagag ctctccgttc tgccaggggg
    16441 gccagggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt tccggcgtca
    16501 tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg
    16561 tgattaaagt ccagctgagc gatggtcgta agttcgacgc gaagatggtt ggcaaagatc
    16621 cgcgctctga tatcgcgctg attcagatcc agaacccgaa aaacctgacc gcaattaaga
    16681 tggcggattc tgatgcactg cgcgtgggtg attacaccgt agcgattggt aacccgtttg
    16741 gcctgggaga gacggtaact tccgggattg tctctgcgct ggggcgtagc ggcctgaatg
    16801 ccgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccgtggt aactccggtg
    16861 gtgcgctggt taacctgaac ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg
    16921 acggcggcaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa aacctgacct
    16981 cgcagatggt ggaatacggc caggtgaaac gcggtgagct gggtattatg gggactgagc
    17041 tgaattccga actggcgaaa gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc
    17101 aggttctgcc taattcctcc gctgcaaaag cgggcattaa agcgggtgat gtgatcacct
    17161 cactgaacgg taagccaatc agcagctttg ccgcactgcg tgctcaggtg ggtactatgc
    17221 cggtgggcag caaactgacc ctgggcttgc tgcgcgacgg taagcaggtc aacgtgaatc
    17281 tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc aacggcattg
    17341 aaggtgctga gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa
    17401 cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt ggcgcgaacc
    17461 agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc
    17521 tggcactgaa cattcagcgc ggcgacagca ccatctacct gttaatgcag taatctccct
    17581 caaccccttc ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt caccacaact
    17641 ccatacatct tcatcatcct ttgggcattt gcacaatgcc gtacgttacg tacttcctta
    17701 tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac caaaatggcg
    17761 caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt aatggatgcc
    17821 cgcgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca
    17881 ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtggc acgccatctg
    17941 cacggtgtgc ggcaggggat taatctaccg ttacggctgg aaggtgaaat tgtcggcgta
    18001 attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt ctgcatgacg
    18061 gctgaaatga tgctggaaca gtcgcggttg atgcacctgt tggcgcagga tagccgtttg
    18121 cgggaagaac tggtgatgaa cctgattcag gctgaggaga atactcccgc acttactgaa
    18181 tgggcgcaac ggctggggat cgatctcaat caaccgcgag tagtggctat tgttgaggtc
    18241 gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact gcaaaatgcg
    18301 ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctgaccga aatggtggtg
    18361 ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg taagcgagtt
    18421 gaacaactga ttacccgcat gaaagagtat ggccagctgc gttttcgcgt ttcgctgggc
    18481 aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa aacgacgatg
    18541 gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga tctgatgtta
    18601 cctgtgttac tcgacagttt gcgtggcgac tggcaggcca atgaactggc gcgaccgctg
    18661 gcgcggctga aagcgatgga caataatggc ttgctgcgac gaacgctggc ggcgtggttt
    18721 cgccacaatg tgcaaccgct ggctacgtca aaggcgctgt ttattcatcg taatactctg
    18781 gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggtaa ttttgatgac
    18841 aggttgctgc tgtatgtggc gttgcagctg gatgaagagc ggtaggttat gcgttaaggt
    18901 cgaggcggcg ctggctcatt cccctcagcc cgaagggtag aggggaatgt ccgagttaac
    18961 cgtcagttcg gcattattta cgcgttagtt tttccagatc ggcttcaatc tcgctgatct
    19021 tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa
    19081 cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac cgtagattcg
    19141 ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg accgttttac
    19201 gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga
    19261 aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga tcaatttctt
    19321 caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct caaaatgagt
    19381 cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact gtgatcgcag
    19441 aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta cctgctttat
    19501 tgtggcgata ttccatggga tggattaaca catttatctt cgttttgcga attacatgac
    19561 agtccgaaac aagatgaaaa agtatttaaa aaagggggga agaagaatga gtaggacgaa
    19621 ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt actcttaatc
    19681 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    19741 aacgataacc gcacagtaga ggctgtattt gccatctttc gacggcagat tacctgaaac
    19801 aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    19861 acgggtgctc tgaccaatgt atacgcccat ggaaatgacg gaaccttctt cgacaatcac
    19921 cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaatga tggttgggtt
    19981 agcctgcagc ggttccagca cgccgccgat gcccacgcca ccggaaaggt ggacgttttt
    20041 accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc cttcatcaac
    20101 atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa taaacgcacc
    20161 ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc
    20221 gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac
    20281 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    20341 ccactgaccg tcaatttttt ccgctacacg cagtgcaccg gaatccagca gggcgatcac
    20401 ctgatttacc gcttcgcggg taacggtgtc tgcgttggct ggcgtgatct cggcacggcg
    20461 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    20521 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    20581 ctgctgcaac tcgttattaa gtgcacgccg gtcagcggtg gcaattatga ataaatcttc
    20641 tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca gatcggcaaa
    20701 aattttcccg actctcgcca gcagtcctgg ctggtcgagc gcgataagct caaggaacga
    20761 tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa
    20821 tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca gtacttgctc
    20881 cagaccaaac cgaataacct catgacgatc tgcggacagc gggctgccat cgggttccag
    20941 cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac
    21001 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    21061 gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca ataccagcgg
    21121 tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat ttgggctatg
    21181 gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    21241 gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat
    21301 atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg
    21361 caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa tgtcagccac
    21421 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    21481 gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg
    21541 gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa
    21601 atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    21661 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    21721 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    21781 gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac
    21841 gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca ttggcaatag
    21901 cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg
    21961 aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc gcagcgtggt
    22021 ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    22081 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg
    22141 aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    22201 gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc
    22261 cttcatcatc cgctcgaccg gctcgttgcc ttcaccactg taattcagac gctgagcgac
    22321 gctaagttgg cgatcgaata acaggcgatt atcgtaacgg ctgacgacca gatgcagggc
    22381 aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt
    22441 taagaagcca aacccaacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac
    22501 ccattgcaga gtatggatat cgcgcaagcc gccagggcta cttttgatgt ctggttcaag
    22561 attgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc
    22621 gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca gttcgaggaa
    22681 gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    22741 cgataaccct tccagcatgc actcttcaag cgtgcgcaca ctatgaccga cttccagctt
    22801 tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt
    22861 tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta
    22921 gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa
    22981 tcgttgcagg agctggtcga taaactcggt gcgcgcctca atcaactgtt ctgcagagat
    23041 cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc
    23101 accgacggtt aattcatcac ggggccagac gcatggattt tgcggttgac cgggcagggt
    23161 ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga
    23221 ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg tcgtgcgaga
    23281 ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca
    23341 ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc
    23401 catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg atggtgaacg
    23461 tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc acctgcggtt
    23521 cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg gagaagcctt
    23581 ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg cctggtttga
    23641 ccatgcgcag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt tcgcccatga
    23701 tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta
    23761 cggtgacatc aatgttaacg atatcgccat ctttcagcag cttcgcatcg tccgggatac
    23821 cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg tgatagccga
    23881 ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct
    23941 cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca
    24001 gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta
    24061 attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc
    24121 acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aacgttttct
    24181 ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg gtgtccatga
    24241 cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg
    24301 gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc
    24361 ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt
    24421 taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc
    24481 agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc
    24541 acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca
    24601 agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag
    24661 cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg
    24721 gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa
    24781 ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc
    24841 gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg
    24901 acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa gcaaacaacc
    24961 tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg
    25021 ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg
    25081 caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag
    25141 aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag aaaggcggca
    25201 agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga gcgaagacaa
    25261 cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta gtacacgttt
    25321 ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg tttaatgccg
    25381 ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc tggtaaaaga
    25441 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
    25501 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
    25561 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
    25621 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
    25681 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
    25741 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg agaacatcaa
    25801 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
    25861 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
    25921 gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg ctgaagtggt
    25981 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
    26041 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
    26101 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
    26161 tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag ttgagactga
    26221 ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcgaaaa ggagccgcct
    26281 gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg gggctgcgct
    26341 gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca gtctcaggaa
    26401 agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa gttgagtggc
    26461 gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga tcgtatggct
    26521 caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat tggtgggggt
    26581 aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt gggcgaccac
    26641 atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc
    26701 tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga cagctacagc
    26761 tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca
    26821 ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga aattgaagcc
    26881 gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat
    26941 ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa agagctgaaa
    27001 gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat tcgtgttttc
    27061 aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta
    27121 atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa gtatcgcggg
    27181 ggcgttagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca
    27241 gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc caaaatggca
    27301 aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag ggctatactt
    27361 agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc aaggattcgt
    27421 aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg cgtagaagcg
    27481 ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct gctggatggc
    27541 attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag cgtaacggta
    27601 gaagattccc gtacactgaa aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa
    27661 aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag cgacatccgt
    27721 gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt
    27781 gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg
    27841 aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc tcaggacgat
    27901 gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc agacaaagaa
    27961 gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt agatccttgc
    28021 ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt tcatgaagca
    28081 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca
    28141 taatcccgaa aacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt
    28201 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa
    28261 acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca
    28321 agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag ccattgttgg
    28381 cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc
    28441 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa
    28501 agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt taccgcaacc
    28561 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact
    28621 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc
    28681 ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg tcgattcggc
    28741 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc
    28801 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca
    28861 ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac
    28921 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag
    28981 tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac tggcgatgga
    29041 ggcgttcgaa caaggtcagg cagcgacgac agcattgaat gccgcaaacg aaatcaccgt
    29101 tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt
    29161 actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc
    29221 gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag gataatccgg
    29281 ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct gcgccatctg
    29341 atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg cttttttttg
    29401 tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg
    29461 ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg
    29521 atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg tgcctttggg
    29581 cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa caacggtatt
    29641 gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc gcaggaagtc
    29701 agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga
    29761 cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg tttgcaagaa
    29821 cgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct gaatattgcg
    29881 gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc tgaaaaggtg
    29941 cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca gcatgtctgt
    30001 atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca tcgcattagt
    30061 aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt tctctggccc
    30121 gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt
    30181 ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat
    30241 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
    30301 ccggtggggt tcgccatcgt aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga
    30361 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
    30421 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
    30481 gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt gctggtgctg
    30541 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct tatttttggc
    30601 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactacgac
    30661 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
    30721 gactctggcg catatatgtt tggcaaattg tttggtaaac ataagctggc accgaaggtt
    30781 tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc ggtaatctca
    30841 tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact catttgctct
    30901 attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt taagcgtgaa
    30961 gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
    31021 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
    31081 ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc atcgttgcac
    31141 tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg cgttgtggtg
    31201 ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga actgataagc
    31261 tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa atgctggatg
    31321 agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat aaatctgtcg
    31381 gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt gctatctttg
    31441 cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag
    31501 cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta aaagccgtag
    31561 atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat aaaattggcg
    31621 atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc
    31681 tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct tcgctgggga
    31741 ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcaacca aactcggcgg
    31801 caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa
    31861 cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc ttagcgttag
    31921 aaatcgaaag gcagggaagt cccttgtctt tgacattaat cccggagagt aaaccgggta
    31981 atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg ccagatgagt
    32041 ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg gacaaaacgt
    32101 ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac
    32161 tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca gcggaactcg
    32221 gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg ataattaacc
    32281 tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc gaaaagatca
    32341 agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc tcgattctgc
    32401 tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga gagttagtta
    32461 ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag
    32521 cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct
    32581 tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag gcgacacggt
    32641 taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca actttgagga
    32701 tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac gtccgaccat
    32761 tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga agcaaaacct
    32821 cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg ccgatatcga
    32881 gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg taaaagctgt
    32941 cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg aaggtgtgtc
    33001 agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg acgaactgat
    33061 ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata
    33121 ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agttactatc tggatcgcgg
    33181 ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata aaaaaggtat
    33241 ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag
    33301 cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg agctgggtga
    33361 gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc ttctcggtcg
    33421 ctatggttat gcctatccgc gcgtacagtc gatgcccgaa attaacgatg ccgacaaaac
    33481 cgttaaatta cgtgtgaacg ttgatgcggg taaccgtttc tacgtgcgta agatccgttt
    33541 tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg
    33601 tgcatggctg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc gtctgggctt
    33661 ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc aggttgatgt
    33721 agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcggaattg gttacggtac
    33781 tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag gtacaggtta
    33841 tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac
    33901 caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct ataatgactt
    33961 ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta cagacgtgac
    34021 gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt atgtacataa
    34081 ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact ctatgggtga
    34141 acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca cgttcaacta
    34201 tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa
    34261 cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag tgacgttaga
    34321 cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg ggcgtacccg
    34381 ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga acttctatgc
    34441 cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga aagcagttta
    34501 cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg cgactcagga
    34561 cggcgcaaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca tggcggttgc
    34621 cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta actcggttcg
    34681 tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt ccagccaata
    34741 ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg gtatcgcatt
    34801 acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt tcaaaaagta
    34861 cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt aagtgttctc
    34921 cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata agatcgccgg
    34981 gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt attgtgaaaa
    35041 agtggttatt agcggcaggt ctcggtttag cactggcaac ttctgctcag gcggctgaca
    35101 aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa accggtgttt
    35161 ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg
    35221 atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat cgcactaagc
    35281 tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag gcttttgagc
    35341 aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt atccagactg
    35401 ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca aacgccgttg
    35461 cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag gttaaataag
    35521 taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa ctacacggtg
    35581 atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta
    35641 cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg tccgcggttg
    35701 tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg aagaatccct
    35761 acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc gcgcagaaca
    35821 ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta tcgattggcg
    35881 ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt
    35941 gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg aacgtaacca
    36001 tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg
    36061 cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca cagattggtc
    36121 gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc
    36181 tggatgacac tattattggc aatggcgtga tcattgataa ccagtgccag attgcacata
    36241 acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg ggcagcctga
    36301 aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat atggaaatat
    36361 gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact gaaccaggcg
    36421 tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg
    36481 tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac
    36541 aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt cttacgattg
    36601 cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta tcttgactac
    36661 taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc gtttcccgtt
    36721 cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa
    36781 tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga ttttcccggg
    36841 tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta aaagcgtagg
    36901 aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc gcttcaagcg
    36961 cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg
    37021 cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat
    37081 gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct ttgtgcatcc
    37141 aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg
    37201 tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc acgttgtcgt
    37261 gaatggtcat actaaaattg gccgcgataa tgagatttat cagttcgcct ccatcggcga
    37321 agttaaccag gatctgaaat atgctggcga accgacccgt gtggaaatcg gcgatcgtaa
    37381 ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg gattgacgaa
    37441 ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg
    37501 taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg ttgacgactt
    37561 cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg cgcacgtgat
    37621 ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg cgcagggtaa
    37681 ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat tcagccgtga
    37741 ggcgattacc gctatccgca atgcgtataa gctgatttat cgtagcggta aaacgctcga
    37801 tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga aagcctttac
    37861 cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca gcgtccatta
    37921 acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg tttaatccgc
    37981 gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc acgaatgcag
    38041 gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg cattgttgaa
    38101 gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa gcgttttggc
    38161 gaactgaagc cagatatttt tgttggtatt gatgcgcctg acttcaatat tactcttgaa
    38221 ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc cgtctgggcg
    38281 tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct cgcatttctg
    38341 cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat cggtcatacc
    38401 atggctgatg ccatgccgtt agatccagat aaaaatgccg cccgtgatgt gctggggatc
    38461 cctcacgatg cccactgcct ggcgttgcta ccagggagcc gtggtgcaga agttgaaatg
    38521 cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc ggatctcgaa
    38581 atcgtggtgc cactggtgaa tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa
    38641 gtcgcgccag acctttcagt tcatttgctg gatgggatgg gccgtgaggc gatggtcgcc
    38701 agcgatgcgg cgctactggc gtcgggtacg gcagccctgg agtgtatgct ggcgaaatgc
    38761 ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa gcggctggtg
    38821 aaaactgatt atgtctcgct gccaaatctg ctggcgggta gagagttagt caaagaatta
    38881 ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac
    38941 gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca gatccgctgc
    39001 aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc gaatttgttt
    39061 atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg
    39121 cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg ctgaatgatt
    39181 ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa gagaaagcgt
    39241 tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac attcttcatg
    39301 cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt
    39361 tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg gtgaaaggcg
    39421 atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg
    39481 aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac aaagggtacc
    39541 caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac catcggcgca
    39601 gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt cgagattaag
    39661 taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg cacagcgact
    39721 actcgatgat cgatggcctg gccaaaaccg caccgttggt aaaaaaggcg gcggcgttgg
    39781 gtatgccagc actggcgatc accgatttca ccaacctttg tggtctggtg aagttctacg
    39841 gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaac gtccagtgcg
    39901 acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat accggctatc
    39961 agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga
    40021 tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt tccggcggac
    40081 gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta gatgagtgtg
    40141 tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc cgcaccggca
    40201 ggccggacga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc
    40261 ccgtcgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac gcacacgaaa
    40321 tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg cgtaactatt
    40381 cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc gacatccccg
    40441 aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaaccgtg cgtcttggtg
    40501 aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat ctggtcaagc
    40561 gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt ccctgatgag gaagaacgtc
    40621 ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt atcaaccaga
    40681 tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg aaagataacg
    40741 gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc tacgcgctga
    40801 aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc cttaacccgg
    40861 aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc gatcaggtta
    40921 tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagatcatc accttcggta
    40981 caatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat ccgtacggct
    41041 ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt
    41101 ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt aaggcgctga
    41161 tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac gccggtgggg
    41221 tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat gaagagggca
    41281 aacatccggt cacccagttt gataaaagcg acgttgaata cgccgggctg gtgaagttcg
    41341 acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg atcaacaagc
    41401 ggcgggcgaa gaatggcgag ccaccgctgg atatcgccgc gatcccgctg gatgacaaga
    41461 aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt gaatcgcgcg
    41521 gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg atcgcactgg
    41581 tggcactgtt ccgccccggt ccgttgcaat cagggatggt ggataacttt atcgaccgta
    41641 aacatggtcg tgaagagatc tcctatccgg acgtacagtg gcagcatgaa agcctgaaac
    41701 cggtactgga gccaacctac ggcattatcc tgtatcagga acaggtcatg cagattgcgc
    41761 aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga
    41821 aaaagccgga agagatggct aagcaacgtt ctgtatttgc tgaaggtgca gaaaagaacg
    41881 gaatcaacgc tgaactggcg atgaaaatct tcgacctggt ggagaaattc gctggttacg
    41941 gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg ttatggctga
    42001 aagcgcacta tcctgcggag tttatggcgg cggtaatgac cgccgatatg gacaacaccg
    42061 agaaggtggt gggtctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
    42121 atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc gtgtatggta
    42181 ttggcgcgat caaaggggtc ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
    42241 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttga
    42301 accgtcgcgt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacatc
    42361 gcgcagcgct gatgaactcg ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag
    42421 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
    42481 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
    42541 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
    42601 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
    42661 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
    42721 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
    42781 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
    42841 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
    42901 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
    42961 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
    43021 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
    43081 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
    43141 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
    43201 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
    43261 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
    43321 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
    43381 cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
    43441 ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
    43501 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
    43561 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
    43621 ctgaacgctt taagatgcca atcatcacct ttatcgacac cccgggggct tatccgggcg
    43681 tgggcgcaga agagcgcggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
    43741 gccttagcgt accgactatt tgtaccgtta tcggtgaagg tggttctggc ggcgcgctgg
    43801 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
    43861 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag
    43921 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
    43981 aaccgctggg tggtgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
    44041 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
    44101 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
    44161 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
    44221 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt ataaagatga
    44281 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
    44341 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
    44401 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
    44461 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
    44521 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
    44581 gcgtcagtac accgacgaat atcttgataa cattacaccg ccgttcacga aagccttgtt
    44641 tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
    44701 atatcaaaaa agcccggttg gctgcctgtt ttatgatttt ttcggcggga ataccctcaa
    44761 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg ctcgaccaca ccgggccaca
    44821 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
    44881 caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
    44941 gctgttgatc gaccgcaatt gtcataaatc gctggcgcat ctgttgatga tgaacgatgt
    45001 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attcttggtg ggatcccgcg
    45061 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccaccacgc aggcacaatg
    45121 gccggttcat gcggtgatta ccaactccac ctatgatggc ttgctctaca acaccgactg
    45181 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtacac
    45241 ccattttcat ccgatctacc aggggaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
    45301 agtgatcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcgct
    45361 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
    45421 caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg cgatgctgcg
    45481 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
    45541 agaggtccag cggttgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
    45601 gcaggtggat gaagccgaat gctggcccgt tgcgccaggc gaacagtggc acggctttaa
    45661 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
    45721 gatggacgaa cagggcaata tgagcgagga ggggatcccg gcggcgctgg tggcaaaatt
    45781 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
    45841 tagtattggt atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
    45901 acgctcttac gatctcaacc tgcggatcaa aaatatgcta cccgatctct atgctgaaga
    45961 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
    46021 tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
    46081 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
    46141 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
    46201 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
    46261 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
    46321 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
    46381 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
    46441 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
    46501 tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
    46561 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
    46621 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
    46681 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
    46741 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
    46801 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
    46861 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
    46921 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
    46981 gcagtggcgg acggaaaatc tgggcgtcac tctgcgcgct attcatgtgc atcacggttt
    47041 aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
    47101 gccgctggtg gtcgaacgcg tgcaacttgc gcaagaagga ctgggcattg aggcccaggc
    47161 gcggcaggcg cgttatcagg catttgcccg caccttgttg cccggtgaag tgctggtcac
    47221 cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg gcgctaaaac gcggtagcgg
    47281 ccctgccggg ctttcggcta tggcggaagt ttcggagttt gccggaacac ggcttatccg
    47341 cccgttgctc gcccgcacgc ggggagaact ggagcagtgg gcattggcgc atggtttacg
    47401 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
    47461 cgtagtgccg ttattgcagc agcgttggcc gcattttgcc gaagcaacgg cccgcagcgc
    47521 cgcactttgc gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
    47581 ctgccaaacg tcgcaggggg cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg
    47641 tcgcgcggcg attatccgcc gctggctggc agggaagaat gcaccgatgc cttcccgcga
    47701 cgcgctggtg cggatctggc aggaagtggc gctggcgcgg gaagatgcct ccccctgttt
    47761 acgcttgggc gcgtttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt
    47821 caccgggcaa agtgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc tggaattacc
    47881 ggcggggctg ggaagcgtac agcttactgc gggaggcaat attcgccctc cgcgtgcaga
    47941 cgaagcggtc agcgtgcgtt tcaaagcggc aggattgctg catattgtcg ggcgcaacgg
    48001 cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac
    48061 cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca
    48121 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
    48181 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggactcgc
    48241 tcaccaccac cgtaccgatt tccgggtggc taaagctggt aattttatcc agacgtagct
    48301 cacgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
    48361 cgctggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
    48421 gctggcaggc gagttcaaga ttatcgtaat catcacaatt gattggttga tacgtatcat
    48481 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
    48541 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
    48601 gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac
    48661 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
    48721 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
    48781 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
    48841 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
    48901 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
    48961 tgatgctgcg cctgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
    49021 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
    49081 tgtggattga gctggggctg ccggatgagc ggcggattaa gaaagcctgc acccaggccg
    49141 cagaagtggc gctgtttacc tgtaatagtc gggcggcgca aatctggtgg cagcaaaatc
    49201 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
    49261 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggcg
    49321 tgatctggtt atcggacgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
    49381 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
    49441 cattcgtgcg cagggcgcgg gcgggcagca tgttaataag acctcaacgg ccattcatct
    49501 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc
    49561 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
    49621 tcaggaactg aaccgcgaag ccgcgctggc ccggctggtg gcagtgatta aagatttaac
    49681 aacagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
    49741 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtgc gcagcggtcg
    49801 ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
    49861 gcctctttac tctgatggga tgtaataacc gggccgaagt cgatacgctt tctccagcac
    49921 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg tgtgctgatt
    49981 gcgaaggaat cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
    50041 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
    50101 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggccaaag
    50161 gcgatgcgct ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag ttcaactata
    50221 cgctggaacc ggcacaatcc agtttacccc tgacgccgat gaccctgcgg ggcatgtatt
    50281 tttatatggc tgatgtggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
    50341 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
    50401 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
    50461 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
    50521 tggggcagta aacctgctta aggcagaaac aaacgcaa
//
LOCUS       DAZFAH010000012        48340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFAH010000012 DAZFAH010000000
VERSION     DAZFAH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.012"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(288..881)
                     /gene="tehB"
                     /locus_tag="ACHJWB_000658"
     CDS             complement(288..881)
                     /gene="tehB"
                     /locus_tag="ACHJWB_000658"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4312339.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVSFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(878..1870)
                     /gene="tehA"
                     /locus_tag="ACHJWB_000659"
     CDS             complement(878..1870)
                     /gene="tehA"
                     /locus_tag="ACHJWB_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4312340.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1994..2974
                     /gene="ydcK"
                     /locus_tag="ACHJWB_000660"
     CDS             1994..2974
                     /gene="ydcK"
                     /locus_tag="ACHJWB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4312341.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2969..3505)
                     /gene="rimL"
                     /locus_tag="ACHJWB_000661"
     CDS             complement(2969..3505)
                     /gene="rimL"
                     /locus_tag="ACHJWB_000661"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO4312342.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3568..3792)
                     /gene="ydcH"
                     /locus_tag="ACHJWB_000662"
     CDS             complement(3568..3792)
                     /gene="ydcH"
                     /locus_tag="ACHJWB_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4312343.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3932..5587)
                     /gene="opgD"
                     /locus_tag="ACHJWB_000663"
                     /gene_synonym="mdoD"
     CDS             complement(3932..5587)
                     /gene="opgD"
                     /locus_tag="ACHJWB_000663"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4312344.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5812..7155)
                     /gene="ydcJ"
                     /locus_tag="ACHJWB_000664"
     CDS             complement(5812..7155)
                     /gene="ydcJ"
                     /locus_tag="ACHJWB_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4312345.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7372..8295
                     /gene="ydcI"
                     /locus_tag="ACHJWB_000665"
     CDS             7372..8295
                     /gene="ydcI"
                     /locus_tag="ACHJWB_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4312346.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8333..9973)
                     /gene="trg"
                     /locus_tag="ACHJWB_000666"
     CDS             complement(8333..9973)
                     /gene="trg"
                     /locus_tag="ACHJWB_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4312347.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFTVVASEVRILASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            10372..10521
                     /gene="hokB"
                     /locus_tag="ACHJWB_000667"
     CDS             10372..10521
                     /gene="hokB"
                     /locus_tag="ACHJWB_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4312348.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(10499..10567)
                     /gene="yncP"
                     /locus_tag="ACHJWB_000668"
     CDS             complement(10499..10567)
                     /gene="yncP"
                     /locus_tag="ACHJWB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4312349.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10593..10766)
                     /locus_tag="ACHJWB_000669"
     CDS             complement(10593..10766)
                     /locus_tag="ACHJWB_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312350.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11011..11541)
                     /gene="cybB"
                     /locus_tag="ACHJWB_000670"
     CDS             complement(11011..11541)
                     /gene="cybB"
                     /locus_tag="ACHJWB_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4312351.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALVHHY
                     FWKDNTLLRMMPRKRS"
     gene            11730..12731
                     /gene="gap"
                     /locus_tag="ACHJWB_000671"
     CDS             11730..12731
                     /gene="gap"
                     /locus_tag="ACHJWB_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4312352.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12773..14212)
                     /gene="aldA"
                     /locus_tag="ACHJWB_000672"
     CDS             complement(12773..14212)
                     /gene="aldA"
                     /locus_tag="ACHJWB_000672"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4312353.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPTIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGETMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14410..15210)
                     /gene="ydcF"
                     /locus_tag="ACHJWB_000673"
     CDS             complement(14410..15210)
                     /gene="ydcF"
                     /locus_tag="ACHJWB_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4312354.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15482..19384)
                     /gene="hrpA"
                     /locus_tag="ACHJWB_000674"
     CDS             complement(15482..19384)
                     /gene="hrpA"
                     /locus_tag="ACHJWB_000674"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4312355.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMLEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19585..20190
                     /gene="azoR"
                     /locus_tag="ACHJWB_000675"
     CDS             19585..20190
                     /gene="azoR"
                     /locus_tag="ACHJWB_000675"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4312356.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20244..21536)
                     /gene="ynbD"
                     /locus_tag="ACHJWB_000676"
                     /pseudo
     CDS             complement(20244..21536)
                     /gene="ynbD"
                     /locus_tag="ACHJWB_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(21550..23307)
                     /gene="ynbC"
                     /locus_tag="ACHJWB_000677"
     CDS             complement(21550..23307)
                     /gene="ynbC"
                     /locus_tag="ACHJWB_000677"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4312357.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(23323..24219)
                     /gene="ynbB"
                     /locus_tag="ACHJWB_000678"
     CDS             complement(23323..24219)
                     /gene="ynbB"
                     /locus_tag="ACHJWB_000678"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4312358.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24219..24824)
                     /gene="ynbA"
                     /locus_tag="ACHJWB_000679"
     CDS             complement(24219..24824)
                     /gene="ynbA"
                     /locus_tag="ACHJWB_000679"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4312359.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(25123..25983)
                     /gene="pdxI"
                     /locus_tag="ACHJWB_000680"
     CDS             complement(25123..25983)
                     /gene="pdxI"
                     /locus_tag="ACHJWB_000680"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4312360.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVTHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     WSTLDGISRE"
     gene            complement(26213..26803)
                     /gene="paaY"
                     /locus_tag="ACHJWB_000681"
     CDS             complement(26213..26803)
                     /gene="paaY"
                     /locus_tag="ACHJWB_000681"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO4312361.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(26785..27735)
                     /gene="paaX"
                     /locus_tag="ACHJWB_000682"
     CDS             complement(26785..27735)
                     /gene="paaX"
                     /locus_tag="ACHJWB_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO4312362.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(27836..29149)
                     /gene="paaK"
                     /locus_tag="ACHJWB_000683"
     CDS             complement(27836..29149)
                     /gene="paaK"
                     /locus_tag="ACHJWB_000683"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO4312363.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVERSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(29176..30381)
                     /gene="paaJ"
                     /locus_tag="ACHJWB_000684"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(29176..30381)
                     /gene="paaJ"
                     /locus_tag="ACHJWB_000684"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO4312364.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRSAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(30381..30803)
                     /gene="paaI"
                     /locus_tag="ACHJWB_000685"
     CDS             complement(30381..30803)
                     /gene="paaI"
                     /locus_tag="ACHJWB_000685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO4312365.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(30793..32220)
                     /gene="paaH"
                     /locus_tag="ACHJWB_000686"
     CDS             complement(30793..32220)
                     /gene="paaH"
                     /locus_tag="ACHJWB_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO4312366.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVKRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAVPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPVMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGTQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(32222..33010)
                     /gene="paaG"
                     /locus_tag="ACHJWB_000687"
     CDS             complement(32222..33010)
                     /gene="paaG"
                     /locus_tag="ACHJWB_000687"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO4312367.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLIRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLAWHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(33010..33777)
                     /gene="paaF"
                     /locus_tag="ACHJWB_000688"
     CDS             complement(33010..33777)
                     /gene="paaF"
                     /locus_tag="ACHJWB_000688"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO4312368.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(33774..34844)
                     /gene="paaE"
                     /locus_tag="ACHJWB_000689"
     CDS             complement(33774..34844)
                     /gene="paaE"
                     /locus_tag="ACHJWB_000689"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO4312369.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(34852..35349)
                     /gene="paaD"
                     /locus_tag="ACHJWB_000690"
     CDS             complement(34852..35349)
                     /gene="paaD"
                     /locus_tag="ACHJWB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO4312370.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(35364..36110)
                     /gene="paaC"
                     /locus_tag="ACHJWB_000691"
     CDS             complement(35364..36110)
                     /gene="paaC"
                     /locus_tag="ACHJWB_000691"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO4312371.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(36119..36406)
                     /gene="paaB"
                     /locus_tag="ACHJWB_000692"
     CDS             complement(36119..36406)
                     /gene="paaB"
                     /locus_tag="ACHJWB_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO4312372.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(36418..37380)
                     /gene="paaA"
                     /locus_tag="ACHJWB_000693"
     CDS             complement(36418..37380)
                     /gene="paaA"
                     /locus_tag="ACHJWB_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO4312373.1"
                     /translation="MHENWNRKGDHVTQEERFEQRIAQETAIEPQDWMPDAYRKTLIR
                     QIGQHAHSEIVGMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCARE
                     DIYQKMLDGRMKYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMV
                     KICKEESFHQRQGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSAR
                     SLAWKIKRFTNDELRQRFVDNTVPQVEMLGMAVPDPDLHFDTESGHYRFGEIDWQEFN
                     EVINGRGICNQERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            37632..39677
                     /gene="paaZ"
                     /locus_tag="ACHJWB_000694"
     CDS             37632..39677
                     /gene="paaZ"
                     /locus_tag="ACHJWB_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO4312374.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            39925..42198
                     /gene="tynA"
                     /locus_tag="ACHJWB_000695"
     CDS             39925..42198
                     /gene="tynA"
                     /locus_tag="ACHJWB_000695"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO4312375.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQDMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMVNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(42255..43754)
                     /gene="feaB"
                     /locus_tag="ACHJWB_000696"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(42255..43754)
                     /gene="feaB"
                     /locus_tag="ACHJWB_000696"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO4312376.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHLHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            43990..44895
                     /gene="feaR"
                     /locus_tag="ACHJWB_000697"
     CDS             43990..44895
                     /gene="feaR"
                     /locus_tag="ACHJWB_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO4312377.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTNGVNLSRTWQEIKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYGRKFR"
     gene            complement(45067..45396)
                     /gene="ydbL"
                     /locus_tag="ACHJWB_000698"
     CDS             complement(45067..45396)
                     /gene="ydbL"
                     /locus_tag="ACHJWB_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4312378.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(45401..45586)
                     /gene="ynbE"
                     /locus_tag="ACHJWB_000699"
     CDS             complement(45401..45586)
                     /gene="ynbE"
                     /locus_tag="ACHJWB_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4312379.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(45583..48222)
                     /gene="ydbH"
                     /locus_tag="ACHJWB_000700"
     CDS             complement(45583..48222)
                     /gene="ydbH"
                     /locus_tag="ACHJWB_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4312380.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT        12150 a        12856 c        11496 g        11838 t
ORIGIN      
        1 gatgattata ttactttcta agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa
       61 agtgttggtg aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa
      121 ggggctaagt caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaacttcgac
      181 tactgtgatt aatttcacat cccttcgata aaaaatatgc ttaccgaaaa gaagaacagt
      241 aaatcatctc agcgtgggga attttcacgc gttttgtgct taccgggtca ttttttacgt
      301 gccagcatcg tggcgaaacg cagtttaata cgattaccgt tggcgtcggt gcggtgcagc
      361 tcgccgacgt cttcattgta tttcaccctc tcccagcctt cgtaatatcg acgtaattct
      421 ccctctttga aggcaaacgg gaagctgacg gtacatggat aatcagcggt atccatcgcc
      481 gccacaatca ggttgtaacc accaggttta gtgcaacgtt gcatattggc aatcagccct
      541 gggatggttt tagcctcaag gaacatcagt accacagtcg aaagaataaa atcgtactgt
      601 ctatcaaatg tgaggttatt cagatcaacg actcgggtgt gtaaattatc cagattttca
      661 atggatttaa tgcgctctac attggcgata ctcatggcat ttttatccca tgcgtcaaca
      721 tcataaccat tggctgccag gtaaagactg ttacgaccat tgccacagcc cagatccagc
      781 gttttacccg gtttaaccac tttcaccgct tccagtactt cagagtgtgt gcgggttaat
      841 tcatatttat cagtaaaata gttttcgtca cgaatgatca ttctttgtcc tctgctttca
      901 ttaaaacggc gcgctcggtt ctgactaaca attttccctg catcagaagc gcaaaagtac
      961 ggatgagcag tattgcaata ataaaattgg taaagataaa cagcggtacc gccagcgtat
     1021 ggaaaaatcc attttcgctg ccactcccca gatgcaaacc ggtggttgcc agtgcagata
     1081 cgccgaacga gaaactccag aatgaagcat taaatggctg ggagagatac catggcatca
     1141 gacgtagcat aaacagcagt tgcagcagtc cataaccaaa aagcattttc gccagcgtat
     1201 caccctcgcc gccgttgacg ctcaaccagg cactacaggc caccagcgca ggagcgagct
     1261 gaatgccgag tgatgtccgc agtgccgtgg gtaattctcc cgaactgcgc agacgctgca
     1321 agatcacagg ttcaaggctt agccatgaga aaacgcctgc gcctaaaaac accagaccgg
     1381 cgtcggtgta gcccaacgca ccacaggcca ttgcgctgat aaagttgttg gcaactgtcg
     1441 gcagatacag tccaggcgtg gtagcttctt cagggtgaga tccgcgccat aatcccgcag
     1501 tttgccaggc ggcataagcc aactgaacca cgacaccaaa actgaacagg cacaccgcca
     1561 gtgggcgaaa ccacggaaca aaaccaatcg ccaccagcat cgtcgttgcc ggaaacaaac
     1621 tcacaaaact gctcatcact ggatggcgca cttctgccag cacgctatgc ggaaagcgta
     1681 taagtcgggt aataaatgcg ctagttaaca atccccagat gatcatcgcc agaatcacca
     1741 gcccatcccc taaccagtgg ctgacctgcc aaacctggct ggcatagcgc caggcaaatc
     1801 ccatccctat cgtccccaac acaataccaa agtagcctgc cggcaaattg agcactttat
     1861 cgctctgcat cttcttactc attttattta atttataaag tatatttgaa atgcatttta
     1921 tggaattttc tcgagcatag ccagagccgc agaatttgct acggttatag taagtcacct
     1981 gtgggaaaac gctatgcgca aatatcgtct aagtgaagaa cagcgagcct ttagttatca
     2041 ggaagatggc actaaaaaaa atgtgttatt acggcagatt atcgccatta gcgattttaa
     2101 cgatgtgata gcaggaaccg ctggcggctg gatcgatcgc gaaacggtgt tagcacagga
     2161 aggaaattgc tggatttacg accagaacgc tattgcgttc ggcggagcgg tgatttccgg
     2221 taatacgcgc atcaccggca ccagcgtgct gtggggagag gtttacgcaa cggataatgt
     2281 ctggatcgat aatagtgaaa tcagccaggg tgcatatata agtgacagcg tcaccattca
     2341 tgactcactg gtttgcgggc aatgtagaat atttggtcac gccttaattg atcaacattc
     2401 tatgatcgtc gccgcacaag gcttaacgcc cgaccatcaa ctcctgctcc aaatttatga
     2461 tcgcgcaagg gttagcgcct cgcgtattgt ccaccaggca caaatttatg gcgatgccgt
     2521 cgtccggtat gcttttattg aacatcgcgc cgaagttttt gattttgcca gtgttgaagg
     2581 taatgaagag aacaacgtct ggctatgcga ttgcgctaaa gtctacggtc acgcgcaggt
     2641 gaaagctggc atagaagaag atgccattcc gacgattcat tacagttcgc aggtggcgga
     2701 atatgccatt gtggaaggta attgtgtgtt aaaacatcat gtcttgattg gcggaaatgc
     2761 cgtggtacgt ggtggaccga ttctgctcga cgagcacgtt gtaatacaag gtgagagccg
     2821 tatttcaggt gcggtgataa ttgaaaacca tgtggagtta accgaccacg ccgtcgttga
     2881 agcctttgat ggcgacacgg ttcatgtccg tggtccaaaa gtcattaacg gcgaggagcg
     2941 cattacgcga acgccgctgg cagggttatt atgaatcgat aatacgcgcg tataagttca
     3001 catcatcata ggcatcattc aggaactcag cctgtttcag gcaaccttca aggataaaac
     3061 cattgcgcaa agcgacctgg ttgctttgcg gattgtccac ccgacatttg atcacgaagc
     3121 gtctaagttc accagactga gcgtaatgat gaatcaatgc ctgcagcgcc tgagaaatga
     3181 tcccctgccc ctgatgagat tcgtccagcc agtagccgat ttcagcagtt ttactcagtg
     3241 gttcaatacg attaaacgag ataacgccga taagttcatc ttctttgaaa atcatgaaca
     3301 ttttggcata gccgcgttga tgcaacatca cattaccctg caccgttttt cgcgtgtcct
     3361 cttcactctg aacgaactgc ggccagttta gcgactgctg taaccaggtt ttatttttac
     3421 agattaactg ataaagaggt gtgacgtgac tttccgcaac agcatgtaat tcaagtgatt
     3481 cgcttacttt tatcgtttca gtcatcttat ctccagtcaa tccatgctgt tgttaaaaaa
     3541 gccgcctggc tggcggctta ggaaagttta cacctctttg acgctctcct gctgcaggat
     3601 tttgagcatc tcatctttta actgtagctt ttgttttttc atgcggacca cttccgcatt
     3661 gtaccctcga ccgtcggaac cttcctttct ggcaatttca tgatcaagtt tattgtgttt
     3721 atcgaacaag gacataaagc gaggattttc gtttttcaga cgggatatta aatctcggta
     3781 ttctggaaac atacgcactc cctgttagtg gctgacaagg tgtatctgaa atacacacca
     3841 tcagattact catttaactt agggaaaaat gcgagcggga tcgtgtttaa ccagacggca
     3901 cctcccgaag aaggtgccga agaaaaaacg attaactcat cacgcggtcg tcaacatact
     3961 gacgtttatc cggcgctggc gggaaatact gatacagcca tgtttcactg atagcgtccc
     4021 cctgacaacg tagatacatc cgcatatcga ccggatcagt ggagtccgaa gtcggatacc
     4081 agtcaaactg aatacgataa ccatcgatgg gttcaatata gagaatttcg atttgcttcg
     4141 cttccccact ggaaagcgta atcaccggct caatgccttt tggcgcggca gctttcagat
     4201 caccaccaac gaaatcgacg gcaaaacgac gcgcccattt ttcgggatag tgttcacctg
     4261 gcgcccaacc ttccgggaaa ccgcccatgc cggtacgcgt cgccataacg cgcgctaatg
     4321 ggcaatgaac aggcggttgc gcactccagt acagacgata ctggaatgca aactcatcac
     4381 ctgcttttac agctttttct ggctgccaga agcagacaat gttatccagc gtttcgcccg
     4441 ttgttgggat ttccatcagg ccgatggtgc ccttacccca cttgttacgc ggctccaccc
     4501 acagacttgg gcgtttgtta taccagccca taatgtcctg ataatgggag aagtcacgat
     4561 ccagttgcaa taaaccaaac cctttcgggt tgttgtcggt gtaagcattg aactgcaatt
     4621 tttgcggatt attcagcgga cggcaaatcc actcgccgtt gccccgccac atggacagac
     4681 gatcagagtc atgaatttgc ggatgaattg tatcgcacat ccgacgttca ttagtaccgc
     4741 agctgaacat actggtcatc ggcgcaatgc ccagctgttt aatgtctttg cgcgcataca
     4801 ggtgattttc cacatccata atcacctgac ttttctcaca atggatagtg aacttatagg
     4861 caccagtaat gctggcgcta tcgagcaacg cataaacggt aaaggtagtt gcccccggtt
     4921 ttaccgtatc aaaccagaag gcggtaaagt cggggaactc ttctttactg tcggtgtaag
     4981 tgtcgatcgc caggccgcgg gccgacaaac cgtattgata tgtatcatca acggcgcgga
     5041 aataactcgc gccgagaaat gatactacat cacggcgcgc cagttcgggg gctttaaaca
     5101 cgcgaaaacc ggcaaagccg agatcgcttt gcccttctaa ttgttttgta tcaacacccg
     5161 catcgttgta tttgaacaac tccgggcgaa agtgaatttc acgcgccaga tgtgttgctg
     5221 gatctacaga aaacatacga acgcggcgac ggaatcccat tcccatatgg aagaactgag
     5281 cgtccagttg acggttctca acgttatgcc agagcgattt ttcggcgtcg tattgaatac
     5341 tgttataagc ctgcggtgtc attgtcgcca gcgtgtcagg taacggacga ggcgcaccac
     5401 gccacgctgt ttgcgctaag tcgtgcgcca ttgactgtag aatggagaag tcaaaacgct
     5461 gggtttgccc gtcggcaata tcagaatctg ccgcgaatgc cgcctgagaa aaaagagaag
     5521 caatgccgct ggtaccgcac acggcggcca tagccattga acctttaata aatcgtctac
     5581 gatccatacc tgaaagtgag tccttctggt gttgtgaatg gtcccgcgct ggtcgttttc
     5641 agcggcaaga aagtgcttat ttatgaccgc tcactctaga caatatttat taataatcca
     5701 attgttgaac tgagagaata tgcataaatc ggcatttttt ttatgtcgat gggatgagcc
     5761 tgattcgttt gtaaaatcag gcaaattttt gcaaaggcgg atggtcagaa tttaaagcaa
     5821 accacagcga cgtttactgc gttcttccgc ttcctggtaa agctggaact catccaaaac
     5881 cggacaaccc aacgcctgct caaatgcttc gcgactggca ttaccgtgac tgcgtgtctg
     5941 cgtttcatta cccagatttg actggaagat ccccgccgcg ctaacgggca agaaatcttc
     6001 ataggtgatg ggttgcgcca ctacccaacc acgttcaatt aagggctgtg gatcgtctcc
     6061 aggatgaatc gcctgacgat gcgcctcacc cgaaggcgtc agacggtacc ggaaccatgc
     6121 caacccttgc tgacgcatta aaaactcact gtcaggaaaa gtgcggaagg tttcctgtaa
     6181 atgcatttgg tgagtgagat tatcctgccc ggttccagcg ttacgcagaa gatcatcata
     6241 cagttgtcgc cctttcggcg ttaatgccac gccacgctgc tcaatttcac caaagcgcgc
     6301 ggtatgcgtg ccctgtttct gccccgcaaa caacaccgtc tcttccagtg ctttaaagct
     6361 ggtctggcgt agtaaaatcg gtacctcgcg gcgcggcggc ccctcgatga gaattttggg
     6421 ttcaattccg cattcaggca tcatcgactg cacccggtca atatccagcg tacgtggcgt
     6481 caggtggttg atatggcatc caggaaaaca gaccacatca gcaatcaacc gatgttcgtt
     6541 gtgcaaggcg cgataggttt cttcatctac cgttgctgac tggtgccagc gaaacgtttc
     6601 cagggcttcc tgcacaaact cctgtgcctg tgtttcgtta aaaccgccct gctgctcata
     6661 ttcctctaac agttgtcgac aacgtggggt gaagatatcg cgctgacgca gaatctccgc
     6721 cgctttctgg cgcaaaattt cgttctcgat aagctcaagg cggagtaagg aggtaaaaac
     6781 gcggaaggga ttacgcgcca gagaagcatc atcaatgggc cgaaatgctg tcgaatgtac
     6841 cggcacccct gcctgcgaga gatcgtaata gctcaccggg tacatcccca taatggcaaa
     6901 catccgccga agagtagcaa gctcttgtgc agtcccaacg cgaatcgccc catgacgttc
     6961 aacattcagt cgcgccagct cgtctgcatt taccattttt tcgtgcagtt gaggattgtt
     7021 ttccagcaca gccagattca catcagctac cagttccagc agcgtgccat attgcggaac
     7081 ttcttgctgg tacatggctg acattgcctg cgaaaactgt tcccgaatct catccgccgt
     7141 gatgctgttc gccatagtgt ctgcctccag tggtcagtaa tctggagtgt aggtaaccgc
     7201 attcactctg gcgggaagaa tttacaaact gtgatctcgc cgcgaaaaca tcaatattat
     7261 ccattttgct gtaacataat tgctttaatt gttaataata ttttgcaatc aagttatcat
     7321 aatcaaacaa cttcacttgt cagcgacacc gcttcgtttt taacatcgct tatggaaaaa
     7381 aatagtctgt ttagtcagcg catccgtttg cgccaccttc atacattcgt agctgtcgca
     7441 caacaaggaa ctttggggcg cgcggctgaa acccttaatt tgagtcaacc tgcgctctct
     7501 aagacattga atgaactgga gcagctgact ggcgctcgct tgtttgagcg tggtcgtcag
     7561 ggggcgcaac ttaccttacc cggcgaacaa tttttaacgc atgcagtcag agttcttgac
     7621 gccatcaaca ctgccggaca gtcgcttcat cgtaaagaag atcttaataa tgatgtcgtc
     7681 agggttggtg cactacctac tgcggcactg gggatattac cttcggttat aggtcagttt
     7741 catcagcaac aaaaagagac gaccttgcaa gttgcgacaa tgagtaaccc tatgattctg
     7801 gcgggtttaa aaaccgggga aatcgatatc ggcattggtc ggatgtcaga tcctgaactg
     7861 atgaccgggc ttaattacga actgctgttt cttgaatcgc tgaagctggt tgtccgccct
     7921 aatcacccgc tacttcagga gaacgtaacg ctaagccggg tgctggaatg gccggtcgtt
     7981 gtatcaccag aaggcactgc gccacgccag cattcagatg cattagtgca gagccagggc
     8041 tgtaaaattc cttcgggttg tatcgaaacg ctgtctgctt cgctatctcg tcaacttacg
     8101 gttgaatacg actacgtgtg gtttgtccct tctggcgcgg taaaagacga cctgcgtcat
     8161 gccacgctgg tggccctgcc tgttccggga catggtgcag gcgaaccgat tggaatactg
     8221 acccgcgtag atgcgacgtt ctcttctggt tgccagttga tgattaacgc tattcgaaaa
     8281 tcaatgccgt tctgaaaggt gaagggatct gtcgatccct ccttgaacat tttcacaccg
     8341 tagcgaaact aactggttca cccgctccgc gaggttctgc cgacacagaa tgtttgttca
     8401 gacggaatac gtccaccgcc tcagttaatc gtgccgcctg ttcttcaagg gacaccgccg
     8461 ctgctgaggc ctcttctacc agagaagcat tctgttgcgt caccttatcc atttcagaaa
     8521 tcgcctggct aacctgagtt atgcctctac tttgttcatc cgaggcggcg gcgatttcct
     8581 gcatgatatg tgtgacactc gcgacggcat caacaatagt gctcatggtt ttccctgccg
     8641 ttgccacctc atccgacccc aggtcaatta acctgactga ttcactgatc aagccttcaa
     8701 tctctttcgc cgcttgggcg ctgcggcttg cgagtatccg tacttcgctg gcgacaacgg
     8761 taaatccacg gccttgctca cccgctcgcg cggcttcaac ggcagcattc agtgccagaa
     8821 tattcgtctg gaaagcaata ctgttgatga cggcggtgat ctcggaaatt ttcttcgaac
     8881 ttgtagagat agcgcccatc gtttttacta caccggaaac cgtctgcccg ccatcgctgg
     8941 ctttaataga agcctcttgc gccagtttgc tggcatgatg cgcgttatcc gcattctgtt
     9001 tcaccgtcgc agtgagttgc tccatgctag cggcagtttg ttcgatagcc gccgcttgtt
     9061 cttcggtgcg agatgacaag tccgcattgc cagctgaaat ttcgctggtg ccacgataaa
     9121 tctcttccgc accctgtcga acagtcccta ctgtcatccc cagtgaatgc tgcatttgct
     9181 gtaaatgacg acttaagcga ccgatttcat tacgacccgc cggttcatcc ttcatcgtca
     9241 gatcgccact agcgattttt tcaatccgtt gtgcggcatg ttgcagtgga cgaatgacga
     9301 tccgacgtag caccataaat gttatcagcg tcatgaccag ggcaagcaca aacgcgccaa
     9361 tcatgaacat cccacccagg cgggtgcgct gatgggccag ttccgccagt tggttggctc
     9421 tggtactacg tatcttaacg gctttgttca aaatatcggt ataagcatta tccagcggtc
     9481 ggatctgctc actttcatga ttgataatcg cttcaaacat gccatttttg gcatatttca
     9541 acataggttg catacccgtg atataagcct gaaagcgttg atttaattca gtgtcgaggg
     9601 cttcatcagc aggtgttttc accggtcgat tctgataagc acgataacct tgctgcgact
     9661 gtttaatctc cgattcggct tgcgcaatat ttcgcttcat tgcttccatt tccgcaatac
     9721 gactcgcagc cccggcctga atcatattga tccgggctga acgcaaaaaa tctgaactgt
     9781 tcgctaaccc ggtccgaatc tcaatctccg cagtaacatc acgctgatcg cgatcggcct
     9841 gccagaggaa atagccagcc agagcagaac ttaatgcgaa tagaactaag ataccgccta
     9901 gaatgcaggc aaataacgga accagcctga tgtgatgcaa aaaacctaat cgatgtgagg
     9961 gagttgtatt cattaccgtt gtctctcgtc caggtttact cctgatagaa agtcatcggc
    10021 acttgacggt aattacttat gaccaaaaac ctctttgcgt cacattttta caacaattat
    10081 tgcagagaaa attaatgcta ttggaatgaa gagagtaacg aatggctaaa ctggctaacc
    10141 cgaatgacga aaaattctgg aaaacagcac gcaatttcgt gaagtgatta ttttcgtccc
    10201 taaggaaacc tcataggatg cctgcgggtg cctgagactt tctgttttca ggctaaccgg
    10261 gaatcagaaa gacgaaggcc ccgaccgata tttctatcaa ccgaggccaa cgaatgaacc
    10321 tagcaattca gatcttagcc tcttacccgc catccggcaa ggagaaaggc tatgaagcac
    10381 aaccctctgg tggtgtgtct gctcattatc tgcattacga ttctgacatt cacactcctg
    10441 acccgacaaa cgctctacga actgcggttc cgggacggtg ataaggaggt tgctgcgctc
    10501 atggcctgca cgtccaggta agggcaagcg cggggatttt ccccgcgcat ctgactagca
    10561 gattcatttt ctcatggcac cccttttaca cttcagttag tgcatgtttt tttcgaggca
    10621 ctaatagaac catcattaca gacaaatttg ccgcctgccg tacagtgtga gatcccccct
    10681 ttcgaaccag aacagggttt gcgcccggca tcggcataaa aggaaacaag cgttcccata
    10741 aacaaaataa gtgccagttt tttcatataa cctccatggt ttatcgaatc ttatgttcga
    10801 tttgaacgtt atacgctggt ttattttgat accgttgatg agatcaaaga tcgggtgaat
    10861 tgattaaaaa taagccagat ggacgtatgg gcaaggatta tggttttatt tgtcatacaa
    10921 ataaatataa taggcgcttc cgatgtagac ccgtattctt cgcctgtacc acgggtcggt
    10981 tttagtacag gcgttttctt taaatatcct tcaggaacgt ttacgcggca tcatgcgtag
    11041 aagtgtgttg tccttccaga aatagtggtg taccagtgca gccgcagcgt gcaatccgat
    11101 gacaaaatat cccagattcg ccagcgtttc atgccacgac tttaagctat caacccgttc
    11161 gaaattggcc tctgaagcgt aaggcatcgt caaaccaaac gcaaaccacg ggttgccccg
    11221 gttatacatc atcaccaaac caatcactgg cagcgcaata aacaggagat aaatcaccaa
    11281 atgtcccaaa tgcgccagtc ccgtcatcat cggttttggc ttaggtataa tcggcggggt
    11341 tgggtatttc agccttaaca aaagacgaac gaccatcagc acgagaattg agatgccgca
    11401 ggaaacatga atcatgttga tgagtggccg atcgctacgt gggaagaaac cccgaaactc
    11461 cattgcgcaa tacgcggcga taaccagcaa aaagaccagc cagtgaatgc tgatttgtaa
    11521 ccttgagtat ttattttcca taacatttcc tgctttaaca taattttccg ttaacataac
    11581 gggcttttct caaaatttca ttaaatattg ttcacccgtt ttcagtacgt tacgtttcac
    11641 tgtaagaatg gagattgccc atcccacaat cctggtctaa gcctggaaag gatcaatttt
    11701 catccgaatg ttcctgacag gagaaaacca tgagtaaagt tggtattaac ggttttggtc
    11761 gtatcggtcg actggtgttg cgtcgattac ttgaagtcaa aagcaacata gacgttgtcg
    11821 ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat gattcaaact
    11881 acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt gatgggaaaa
    11941 gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg aaaggtgcag
    12001 aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag gcgcatcttg
    12061 atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa actatcgttt
    12121 ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg gcgtcatgca
    12181 ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg atagaagtcg
    12241 gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat ggcccacgtg
    12301 gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac actacggggg
    12361 cggcaaaagc cattggtctg gtgatcccgg aactgagcgg caaactgaaa ggtcatgcgc
    12421 aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc ggaaaaaaag
    12481 tgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac gagtcatttg
    12541 gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc ggttcggtgt
    12601 ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg aaaacggtcg
    12661 cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc gaaaaattcg
    12721 ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact cattacgact
    12781 gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca tctgcgccgc
    12841 caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag ttttcacggt
    12901 tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg ttcagatttt
    12961 gggtatagat tgatgaggtc aggccgtaat cactgtcatt agccattgag atagcctctt
    13021 ccagcgtgtc aaatgcgacc accggcagaa ccgggccaaa ggtttcctca tgcataatcg
    13081 acatttcctg gcgaacatcc agcagcaatg tcggcggata ataatatcct ttcccctcta
    13141 ccgctttgcc acccaacgcc actctcgccc cttcttctac tgcgcgcgcc actttttgct
    13201 caaccctttc cagcgccgcg gcgttaatca acggccccat cgcaatgtcg ttgcgttcag
    13261 cggggttacc aaattgaacc gcctgcatcg tttcacccag ccgattgacg aactgatcat
    13321 aaatgccttt ctgtacataa atacgttctg cacagttaca cacttgccca ctattaatga
    13381 cgcgtgaatc aacgatggct ttgactgcca gttcaagatc ggcatcgtcc attacgatag
    13441 ctggtgcttt accccccaat tccagacaca ctttggtgat gtttttcgcc gcagtcgcca
    13501 tgatcttctc acctgcagag acgctgcctg tcatactgac cattgcgacc tttgggttac
    13561 ccgccagttc ttgcccaacg gtttcaccac gccccagtac aaggttaaac acgccgcgcg
    13621 gaaggcctat ttcatcgacg attttggcga atgcaatcgc attgtttggc gtaaattcac
    13681 taggtttaat gacgatggta ttaccggtca aaagagcggg agccattttg cgggcaatga
    13741 ggaagaacgg gaagttccac ggcagaatgc cggtagtcac accaagcgca cgtttaaaca
    13801 aaagaatatt ttctcctgga cgatcgcttt gaataatctc gccctcgtaa cgccgtgccc
    13861 actccgccat gtaatcgata tagtcggcag taaaagccac ttcgacttca gccagctgct
    13921 ggatcttgcc cccttcttca acaatcagcg cactgatttc actggcgcgt tcgcggatcc
    13981 cggcggagat tttgcgcaac caactggcgc gttcaatagt aggcaacgct tcccattctg
    14041 gttgtgcacg ttctgctgca tcgattgcct tacgggcatc ctcggcctga ccatcgggta
    14101 tgcgggaaat gacagcctct gtagcagggt ttaccacatc aatccatgcg tctccacgcc
    14161 aggtaacaaa ctgtccatcg atatacatag gatgttgaac gggtactgac atgggcgact
    14221 cctgtgattt atatgtttgt tttcggtgaa tgcattgtta attaacatca atcatcagag
    14281 atgacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
    14341 ataaaaggca gaaataaaag tcaggaatca tgcccgtttg caaacaagtg gttcacaaac
    14401 gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt
    14461 cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
    14521 cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc cagtgagtag
    14581 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
    14641 tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
    14701 attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg tggggtcctg
    14761 tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgcga tgctaaagcg
    14821 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
    14881 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
    14941 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
    15001 accaccacta atcagtaaag gaatttgctg atcgcgggca atcttacatg ccgcgtcgat
    15061 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
    15121 ggagaaatca tcctgcgcca gccactgtcc gataacattt atagcatcta tggttgccgg
    15181 cgaaagcgtc ggaaacgggg taatgttcat ggctcctcct tatcgttccc ttaagaatac
    15241 tgcgtgagat aatagtgaga agcggagcac tctgaaagga ggaagtcgta tttccttaac
    15301 cgccactccg caagcctgac atatcgttta aggaaatgct gtagataaac attattcatc
    15361 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt ttcgcttacc gttttagaat
    15421 taataaaaaa cccggcgcta aatgcttacc gggttttaga tttatctggc aaatagctgc
    15481 gttaaccgct aatctgttcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
    15541 taccaagttg ttgagcgaag taactaacgc gcaactcttc taacatccaa cggatctctt
    15601 tcacgtcttc atcctcacga cgtgcgggcg gcagtttgtt gatccattgc tgccacgcct
    15661 gctggacgtt ttcgactttc agcatctgag cacggtcgcg atgcggatca accgccagtt
    15721 tttccagccg tttttcaatc gcctgcaaat aacgcagcgt gtcgcccagc cgtttgaagc
    15781 cgttaccagt gacaaaaccg cgatacacca acccgcccat ctgcgcctta atgtcagaaa
    15841 gccccagcgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacaccg
    15901 cagtgaggat ttgctcgacc tgcttagcaa tatccaccac cgtgtcattc agttcggcgc
    15961 gcactttttc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
    16021 cgatcaattt atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
    16081 acgggttaaa gtacagtcca agcttggctt tgtttggtaa cttctcatgt aaatacttga
    16141 tcggcgaggg aatatttagc agcagtagac ggcgaagacc gttccacatt gcctgttttt
    16201 gctccagcgg gttatcaaac agtttgatcg ccacactgtc acgttcatcc accagcgccg
    16261 gccacgcctt cactttatag ttgccgcgct tctgttcgta gctttccggc agttgcccaa
    16321 agctccagat atgcagcccg ctctgctcga taccgtcatc cgccaccgcg gacagcgttt
    16381 cctgcacttt gcctttcagc gcatctttca gatcctgtaa tgagcgccct tcttttagct
    16441 tcttattttt gtcgtccacc acgcggaagg tgattttcag gtgatcgggc acctgatccc
    16501 agtgccagtc ttcgcggtca acggtaacac cggtcatccg ccgtaactcg cgctcaaggc
    16561 tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg
    16621 gtgcgggcac aaaattacgg cgtaccggct ttggcagtga tttaatcagg gcgatgatca
    16681 gctcacgacg caggccgggg atctgccact caaacccgct ctcctcaacc tggttaagta
    16741 acggcagcgg gatatgcacc gtcacaccgt cagcatccgc gccgggctca aactgatagc
    16801 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
    16861 tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc
    16921 ggctgacttt tttccaccag ctatcgaagt ggcgtgcaga gatcacatcg tggctgatgc
    16981 gctggtcgta gaactcaaac aacgtttcgt catcaaccag aatatcgcgg cgacgtgatt
    17041 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
    17101 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacgg cataacgccg
    17161 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
    17221 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgaggttcg ctgtaggtgc
    17281 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
    17341 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
    17401 tgaataaacc agaaccggga aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
    17461 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
    17521 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctggc
    17581 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac
    17641 gacacaggcg acggaaggcg ttggaagaaa gcgccttttg ctgctcgcca agataattcc
    17701 acagattcac aaacgcgagg aagtcagact ctttgtcgtg gaagcgacga tgtttttcgt
    17761 ccgatgcctg ctgtttatcc atcggacgct cgcgcggatc ctgaatggaa agcgcggacg
    17821 tgataatcat cgcctcacgc acgcagccat gtttttgcgc ttccagcacc atacgcgcca
    17881 gacgtgggtc gacaggcaac tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
    17941 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
    18001 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
    18061 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
    18121 ccgggcgcga aaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
    18181 gcccacagcg gcctttacgc tgattggcag acgcctgcga aatgggttca atcggcaaac
    18241 gctgcacttt ggtgcgatag ctgtagcggc tgatacgtgc tgtaccgggg tcgataacgt
    18301 atttaatccc cggtacggtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc
    18361 gtccgctgtg cgactggaac acgcggttct gttcgctgtt cgaaagccgc gcataaagcg
    18421 gcaagatttc ggtatgacgc agatttagct tgttcagcgc atcggcggta tcgcgaattt
    18481 cccgctcacc actcataaag atcagaatgt cgccagggct ttcctgactc agttcgtcta
    18541 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc gtcggcttct tcaacaatcg
    18601 gtcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
    18661 taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt
    18721 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
    18781 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
    18841 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca ctaacgtgat
    18901 cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa
    18961 tacggttcgc caccgttctt gccgccaggc gacgcggctg ggtatggccg atcagtcctt
    19021 taatcccgcg ccccagctcc atacagattt tcggcaactg ggtggttttg cctgaaccgg
    19081 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagtatg tcctgttttt
    19141 tctgactaac cggtaaattg tcaggataag tgatttccgg tcgtgccgct tcacgcagca
    19201 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
    19261 catcaggatt tttaaccttc ttaacaccgt gcagacgccg agaaaaacgc agtctgtcgc
    19321 gcagcatcag cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt tgttgttctg
    19381 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
    19441 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
    19501 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccca
    19561 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
    19621 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
    19681 cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat
    19741 ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
    19801 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
    19861 gcggcaccga tgtataactt caacatctcg actcagttga aaaactattt tgacctggtt
    19921 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
    19981 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg
    20041 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
    20101 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
    20161 gcagccatcg acagcattgt tgctgcataa ccggacacgg tcttgatgtg gactgtagat
    20221 attcagtcca catctcaatc cacttacctg ttttcccata atttcagcat cgctttgtgc
    20281 tcttccttaa gcacaatctg cgggcgtctg gctcgaatat agctaatcgc ttcattaacg
    20341 gttttacagt gtccgtaaca taacagccat gccgccacta ccagcgcact gcgcgataat
    20401 cccaacgcgc aatggaccag aacgctgcct tgctcttcgc gtaatgtttc cagcatcgcc
    20461 acggcctgtc ggagctcccc ctcttccgga accaccagat ccagcatcgg tacacagaaa
    20521 tagagtcgat cttttgtcgc tcgtccgcga gggaattcaa aggtgacgtc cagaaccgca
    20581 ttctgtgccg gaatatgtcg tggaaacgcc cctaaataaa caccagcagt cattttgctc
    20641 accggctcca ggcgacgaca aaacaaacgc atagacagtc acatcccgat gcgccagggc
    20701 aatgtcagcc agtaaacggc gggcgggagt ttcccctgac tatctttccc tgttgttatc
    20761 gcgccaagcc caccgtaccc acggccaata atgagtagcg ataatactgg ccaacataac
    20821 cagactgacc accataactg aatcatcacc attagctcca tcaacacaat gcacgagccc
    20881 gcgcctacga catatggcag tgctattttg attcgacgtt gatcaggttt ctgataattc
    20941 caacgacgat cgacgggcac catccagtca atcaacatac ctaccgccag ccctgtgatg
    21001 acatcaataa aatgatgctg ccaggtcgtc agagtagaaa tagcgatgag taaaaaccat
    21061 ccgccacaga ctttacgcca cctctcagcc agatgctgac gaaagtgacg ccaaagtagc
    21121 cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa cagttcaagt
    21181 tgcgaaaata gccatcccgt caccccactc acttcaggac ggataaaact aaacttcagc
    21241 ggatagagca aaaaaccgca gcaggccatt accgttgcca gaataagccg gtggacaagt
    21301 cggcgctgtt cgaatgtcgt gctacaaacg aacagcgaaa atccatataa aagatccaga
    21361 ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca accgaatacc
    21421 tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca
    21481 taggtgaaga agaaaaacgg ggccagcaac aataaccagc cagcgccttg tagcaatacg
    21541 ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatac cccactcatc
    21601 aatccgttgt gtgcatttat caaatccggc atcacgcacg agtgaatcca tctccccttg
    21661 cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca ccccggcaat
    21721 catctccagt tgagggtgcc acggctgccc ggtgtagatg agaatgccgc ccggttcgat
    21781 ggcattggca agacctgcga gtgagttttt tacctgctca ttttcgggaa acagctcata
    21841 cagtccagag acaatcgcca gcgtaggccg cggagttaac gcgctgagtt cctccgggtt
    21901 aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg caatcatctc
    21961 ttgcccctgt gcaacattta actcactgta atcacgtaat aaaatatcgc ttacggcagg
    22021 ctcgtttgcc agtgcatcca gtacatagcg cccatgccct gcggcaatgt caaccacgcg
    22081 gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcagta tttgtaaatg
    22141 ggttttgcgc tggcgaatac cgcgccagcc aacactgttc aggtagattt tgtcgattaa
    22201 gcgcccgaat gcgttactgc cttgcggttg attttgatag acataatcca gcgaactgcc
    22261 tgaatcaaag ccagtgctca ttccgagatg caggcccgca gagtgcgtcc cgaacaattt
    22321 catcgcttta cgcataaagc gatacgccaa atcaaccggc gataatggca cgggtccacc
    22381 tgaaaggagg cgccagcgat ccgctgatgg tccagtgcgg tcttcatgct gataatcaaa
    22441 tttttgtgac ttgttagcat ataaacgact aataaagctt tgcatttttt caaatgcctg
    22501 cgccctgttc tcttcaccca acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt
    22561 cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgta ccacatagtc
    22621 atcgcctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatctc taataatacg
    22681 ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg tgatcagcgg
    22741 atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgtc ctttcacata
    22801 ggaattaata aaaaacagac cacgcagacg atgccatagc gtcagcgcag gacgtgccag
    22861 cggcacatac aatttaacct taaaggccgg agaagccagc accagcccgc gaattgcagg
    22921 cgcataatca tgaacccatg tggcaaccag cactgcgccg acgctttgcg cgatcacaac
    22981 cacctcttcc agtccaacct ggctgtcgct ggcagcaaaa cggacaaatt catcgacatc
    23041 ccgcactgaa cgcgcaagag atgggctata accacgcggc cccgaacttt ttccatgccc
    23101 tcgggcatcc caggcataaa aagcagtatc tggcatcgcc agttcatcaa cgagatgttg
    23161 tagacgacca gaatgttcat gcccgcgatg aaataagacg atgacctttt tcgccccggg
    23221 ctgtaaagcg ggccagtgcc gataaaacaa cgctgtatta tcactggtgg taaaaaaatg
    23281 ttccccaggg atgcgtgaat tttccatttt ctctattttc cttcagtaac agcagtagcg
    23341 tataaaataa aaaaataccg gagcggtgaa aattaatgag tcaatcctgt caaggagtcc
    23401 gccatgtcct ggtaatagtt tcccactatc tttaacacca atatctcgtt tgatggctga
    23461 catcactaca tcgccgcaaa aaccactaat accaattaac aaacccgcta ataatgcctg
    23521 taatgtattt agcggtgtca gcaacggtcc aataatcagt gacgcaatca tggtggtgat
    23581 gacgcccccc accagacctt ccagcgtttt tcccgggctg actttgggga ccacttttct
    23641 tctgccgcag gatttccccc ataaatactg tgcaatatcg tttgactcgg ttaacgccaa
    23701 aagaaataac accagtaacg ccccgccctg gatattcatg gatggcaaca ttaataacca
    23761 ggcggcatga ctcaaggcga aaacggtcat tatccagccc caaaaaatag cgctcacggt
    23821 atgcagaaaa ccggagggtt cacccacaaa aactcgccag gtggctaata tcagaaaccc
    23881 agacaaaggt ataaataaca gaaacagctc aaagcaatta aaaccgataa gcaaatagtt
    23941 aagaggaata acccaatata accaccgcgg aaaatgtatg gatataagcg tacaatattc
    24001 tttcaatgcc agaaaactta tcagcgcaaa aaacgtcaac gtcatccagc gtggacctga
    24061 aatcaccagt gaaaaacaaa tgacgatgcc ccaccaggta cgaatacgta atgtaacctc
    24121 accccctttt ctctccggta accgccatag cagaaaacgg tttatcagcg ttgctaaaat
    24181 taacaacgcg aaaagtgttg ccagagattt ttccagcatt atctttcagc actcataaaa
    24241 agcacactcc gacaacgatt aatcgcagtc cagagaagca gaattgaagc aatgctccat
    24301 aaaagattat tccattgcat ccactgtgga taaatggcaa cggccagccc ccacaaccca
    24361 aatattaacg cacgatcgct tttgccgaat ggaccggcat aactgcgaat accgttaatt
    24421 gtctgcgcga gtaaaccaca aaactcagtc aatatggtgc aaaataacat gagtattacg
    24481 agtgatgcgt tactttccgg taaaaataaa aacggtaaat agagcgcaat atcggaaata
    24541 acatcgccag tttcattcaa aatcgccccc agacgtgttt gctgattgca ctcacgcgcc
    24601 aacatgccat ccagcgcatt gagcgccata cggataaaaa gcacgatggg caatagcaaa
    24661 aagaggatgg gttgtgccac caacaccaac agcaatccgg taaaaagaga aagcgccagt
    24721 gccgtaagag tgatgtgatt cgctgtaacg tggtgcttat aaagccaaaa catcgtcggc
    24781 cttaacagcg actgaaagag cggttttatc tggtagagcg tcatttatca ttccgtgata
    24841 ttcagtttgc tctatggtgc atcaattgtg atggcggaga tatcacaacg cggagcatca
    24901 acgtaaattc ggaatatatc aagatgtggt ggatggtttt cctacacatc tgtttgatta
    24961 ccgactggat gtcagtgaga taccatcgat cactgtacga atcaatgaat ccattgcctt
    25021 ttccctgatt atccagccgc aaatcctggc cttaaacata tgcactccat gccattaaaa
    25081 aagaaacaaa agggaaatgc atctttccct tttgtatatt cgttattctc gcgaaatacc
    25141 atccaacgta gaccacactt cctcagaaag atgcaatttt tcagcagcca tattctcccg
    25201 taaatgcgca acagaagacg tccctgggat cagcaaaata ttcggtgaac gttgtaacag
    25261 ccacgccagc gccacctgca tgggtgttgc acccaggctc gcagcaacat cggaaagtgt
    25321 ggacgattgc agcggtgtaa agcccccgag cgggaagaac ggcacgtagg caatgccatc
    25381 gtgggccaaa gcatcaatca ttgcatcatc agcacggtgc gcgatgttgt attcgttttg
    25441 cacacagaca atttcggcaa tcttgcgcgc ctctgcaacc tgcgtcggtg tgacattgct
    25501 caggccaata tgttttacca ggccttgttg ttgcatctct gccagcacgg tcaggctggc
    25561 ctcaatcgat ccttccgctg ggccatgacc atcccccatc ataacgcgca ggttaaccac
    25621 atccagcacg tccagcccga gattacgtag attatcgtgc accgcttttt gcagctctgc
    25681 cggagaaaat gcgggcaacc aggaagcgtc ctctccacgc cgcgcaccaa ttttagtgac
    25741 aattgtcagg tcgtcagagt aaggataaag cgcttcgcgg ataatctgat tggtgacgtg
    25801 cggaccataa aagtcgctgg tatcaatgtg agtgacgccc aatgccagcg cctcacgcag
    25861 cacagttata gcgacgtggc gatctcttgg gggaccaaaa accccaggac ctgccagttg
    25921 catcgcgcca taaccaagac ggttaacgga ttttgtaccg agagtaaatg tattgctgct
    25981 catcgcaccc tgccctgtaa taaaacggat tgagaaaaat cagtatagaa acgaaaagag
    26041 ataaataatg cgaaggcgta aacaagatta ctggatgagt tgctctattg tcctgaaaaa
    26101 cacaaaacaa aacgcgtcaa gtaccgcatc cggcaataaa aaaccgcaac ctgtatcagc
    26161 gttgcggttt aggtatcaca tgctaaaaat tagaatgttc agtatctgga cgttactgtt
    26221 tcggtcgcag attctcatca aataccagac gtttcctgtt cggttcaact tcccgcaatg
    26281 gctcaacttg atgcaacgtc tgcttacaac gagtcaccag cacctgatac tcatgcgtac
    26341 cctgcttttt ccatgccagc tcctgctcac tgagttcacg aatcgctttc gccgggctgc
    26401 cgacaatcag gtaattggca ggcatttctg ctttggcttt cacaaatgcg gatgcgccaa
    26461 caatgctgtt ctcgccaatc accgcgccgt ccatcactac cgcgttcatt cccactaatg
    26521 cattgcggcg gataatgcaa ccatgaagga tagcgctatg accaatatgc ccatcttctt
    26581 ccacgacagt atcctgttcg ggaaaaccgt gcataacgca attatcctga atgttcgcgc
    26641 catctttcac tacgatacga ccaaaatcgc cacgcagact ggcatttggc ccaacgtaaa
    26701 cgcccttgcc gagaataaca tcgccgatca ataccgctgt cggatggaca aaactctctt
    26761 ctggcacaac cggagtcaga ccatctatct ggtaaattgg cataacgcct cctgttcaat
    26821 attcaaaccg ccaaaacgtt gaaaatacag gcttcccggc gcaggcagtt caccgaccga
    26881 ggtttcacct ttttcactaa cgaacgctaa agcagcaggc gctacgcgct gataaatgtt
    26941 gatacacagc tgacgcgccg tatgccctgc ccagtgcgcc ggaagcaact cctctggcaa
    27001 caatgggtct ttaaggacga cacggcgata aaaatggatc agtaaaagct gaatatgaaa
    27061 tgcacgctcc ggggttaact cgtctgctgc ctcttttaac agcggcacca gcgggcggaa
    27121 tgactgaata aaggtttcgt acatggcatt ttgttcagtt aaatgccagc actcttccac
    27181 tctggcacgc agtgctgcac gagaaagcgc cagtggtatt tgcgcttcaa aacaaatcac
    27241 gttatccgcc acccccgctt catgcaaaag tgtctgtaca tccgccagtt tttgcgacgg
    27301 cgatgccatc aggctgggtg ccagtgcgcc aaaaccttgc cagatcaact gctttttgac
    27361 atcagccagc gttgatttat ctaaaccttc cgagagcaac aggagccatt taccatccca
    27421 tgcaggttgc tctgcgcgat aaattttact ttctgcccgt cgcgtcaggc gtaagccttt
    27481 atcactgagg ctatagaaac tgcgtcgccc gatgcgggaa acatccagcc agccttcttt
    27541 attaagacga aacaaagcgg tacggacgaa acgctcacca aatcccagcc cttccagcaa
    27601 agcagccaga ctacccagcc agatttcacc accacggtgg gaaagcgaat caccatacag
    27661 agaggagatc aaagatgtgc cactgacagg aacagcgttt acagcatgtt ggataaaagt
    27721 atcaagttta ctcattcgat tcagtctgtt gttattgttg tgatttatga atcatagcac
    27781 aggtcgtatc gacttctggc cctggcgctt taccccacca gggccagacg acacgtcagg
    27841 caccaacaat attgcgcaga tcaaacaccc gacacgcctt gccttctgaa cgcgggatac
    27901 tgccacagtt aacgatcatc acatcggtag agatccccac catcgactta atccgatggc
    27961 gcagctggtg gcatacctgg caacgctgct catgcgtcaa tgtcaggcta ctttctttca
    28021 actccacttt cacagaaagt gaatcaagat gcccccggcg gttcacctcc agttggtaat
    28081 gcggcgataa atgttcgaat ttgacaatct cttcttccag ttgtgacgga aagacattta
    28141 caccgcgaat aatcaacatg tcatcgcttc gtccgctgat gcgatccatg cggcgcatag
    28201 tccgcgccgt tcctggtaac agtcgggtta aatcacgcgt gcggtaacga atgaccggca
    28261 atgcttcttt ggtcagcgtg gtgaataaca gttcgccatg ttcaccatcg gcaagcggtg
    28321 tgccgtcatg aggattaaca atttcaggat agaaatgatc ttcccaaata gttggaccgt
    28381 cggtagtttc cagacactcc atcgccaccc ccggccccat cacttctgac aggccataaa
    28441 tatccagtgc ggtgatcccc agacgccgtt caatctcttt acgcatggcc tgtgtccacg
    28501 gctcagcacc aaacacgcca acccgcagcg agcaaccgct ggcatcaccg cccaactgcc
    28561 gctccagctc ttcaatcagg ttaaggcaat aagatggcgt aaccatgatc atatctggtt
    28621 gaaaatcgcg gatcagttgc gcttgttttt ccgtctgccc gccagacatc gggatcaccg
    28681 tagcgcccag acgttcggca ccatagtgcg cgcccagccc accagtaaac aggccgtaac
    28741 cataggcaac atgaattttg tctttgggcg agccccctgc cgcacgcaaa gaacgctcta
    28801 caatattggc ccagttatca atatcgtttt gcgtataacc gacaactgtc ggtttacctg
    28861 tggttcccga agaagcatga atgcgcacca cttgttccat cggcacggca aaggtgtcaa
    28921 agggatagtt gtcgcgcaga tcctgtttgg tagtacaggg aaatttacgc aggtctgaaa
    28981 gttccctgaa atcatcagga tgtacgcctg ctgcgtcgaa tttgcgccga tacatcggga
    29041 cattttcata cgcgtgtttg agcgtccatt tcagacgctg tgtttgcaac gcctgtaact
    29101 catcaacaga cgcggtttca atcgggtcaa gctttgtatt ggttatcatt gtagggtact
    29161 cgcaggttga tatgctcaaa cacgctccag aatcatggcg atgccctgac cgacaccgat
    29221 gcacatggtg cataatgcgt aacgaccgtt acgccgatgc agctcatggc tggcagccag
    29281 tgccaggcgg gcaccactca ttcccaacgg atggcctaag gcgatagcgc ctccgttggg
    29341 gttaacatgt ggggcatcat caggcagccc caattcgcgt agtacaccca acgcctgggc
    29401 cgcgaacgct tcgttcaatt caatcacgtc catatcgtga atactcagcc ccgcgcgttc
    29461 cagcacccgg cgagttgcag gcaccggacc aagccccatc aggcgcggtt ccaccccggc
    29521 ggttgccatg gctacgatac gcgctcgcgg tgtcagtcct tgcgctgctg ccatctgttc
    29581 actggcaata atcaacgcag cggctccgtc attcaccccg gaggcattgc ctgcggtgat
    29641 cactccattg gcacgaaatg gtgcttttag tccacgtaac tgttccagcg tcgtttccgg
    29701 gcgcaaatgc tcgtcatgtt gtatttctgt tacaacacct ttcttatttt tcaacacaac
    29761 cggaacaatc tcttcagcta aaatgcctga agattgcgct tttgccgaac gttgctgact
    29821 gcgtagcgca aaactatctt gatcttctcg cgagattttt aacagttcag ctacattctc
    29881 tgccgtttcc ggcatgctgt cagttccaaa ttgttgagcc atgagcgggt tcacaaatcg
    29941 ccagccaata gtggtatcaa acatctcagc ctgacgagaa aatgcactgg ttgccttgcc
    30001 cataacaaac ggtgcccgag acattgactc cacgccaccg gcgatcagca aatcgccatc
    30061 gcccgcttta atcgcccgtg cggcgaaccc cagtgcgtcc agcccggaac cacacaagcg
    30121 gttaatggtt gtgccggaaa cactctgcgg cagccccgcc agtaaagtcg ccatccgggc
    30181 tacgttacgg ttatcttctc ccgcctgatt agcacagccg aggatcacat catcgataca
    30241 ctccgcatcg agacgcgggt ttcgcaccag cagctcccgc aaagggatag cagccagatc
    30301 gtcagcccga acacctgata atgccccacc atagcggcca attggcgtac gaattccgtc
    30361 acagataaag gcttcacgca tcaggcttct cctgtaatgg tgccgccgat gcggtgagat
    30421 ttaccgcgaa acagcgcaac cgttttttgt tgttggttaa caatttcgat gtcgtaaaca
    30481 ccggtttgct tgccctgatg acgtacctgc gcagtagcag ttaaggtgtc tccggcaaag
    30541 cctggacgca aaaaatcaat cgtgcaggca gaagcgacgg ctgccagccc ctggctattg
    30601 caggcgtagg caaaggcagt atcagccagt gaaaatagct gcccgccgtg gcaactttga
    30661 tgaccattaa gcatttgtgc agtgacggtc atggtcacta cagcaaagcc ttcatccatt
    30721 gagataatgt cgatgccaag cgctttagcg caggcatcgt tctcatacat tgcatgggca
    30781 ttttgccagg ccttatgact cataaccgct ctccagaagc gctcgttggc gcagcaatga
    30841 acatgggcga tagcgttctt cgccatagtg atgttgtaga ttttcaagga gccttaatat
    30901 tcgctgccag ccaagttgcg ttccccaggc aagcgggcca tatggataat tcaccccaag
    30961 acgcatggcg gtatcgatat cctgttcaga ggccacgcct ttttgcagcg catcaagggc
    31021 ttcattgatg atcattgcta ccgttcgcca aatcagcatt actgggtaat ctgcaatttg
    31081 cagcactgtt ttgccctgct gttgcaggta ataaatggcc ttgcgggtcg ctgagtccgg
    31141 gttcaccgct gccgcagcaa tggtcaccac cttgcccgcc attttgtcga ccactaccac
    31201 cgggcgtgct agtcgtattg ccagcgcctg tgccgtctcg ccttgtgtct caatcaataa
    31261 aacatcgtca atttccgtga caccgtcact tttcttttct actttcattg ggctaaaact
    31321 gtcgcttacc gcttccaggc caacaactgc ctcacgttcc gcgcgccagt cgtacacgcc
    31381 cagcccactt ttcttgccca accgtccacc aatcaccagt tcctgttgca ccagcgaagg
    31441 taaaaaacga cgctcctgcc agaaagcgtt aaacaccgaa caggtgacag caaaattgac
    31501 gtcctgacca atcagatcgg ttaattccag cggccccatc gggaaaccag cgccatcgcg
    31561 aagtgcagcg tcaatcactt ctggtacagc aacctgctct tccagtgccc gccaggcctc
    31621 ggaataataa ggacgcgcaa cacggttaac gataaatcca ggagtcgaat gacagcgcac
    31681 aggctgctta ccccaactca acgttagttc acacaactgc tcaacaactt ccgccgccgt
    31741 tgccagccca ctgaccacct ccaccaactt cattaccggt gccgggttaa aaaaatgcag
    31801 ccccgcaaca cgttcaggat ttttcacctc cgcagcaatc gcggtaatag agattgacga
    31861 tgtgttagtg gtcaatagcg tttgtggcgg gcaaacttcc gccagctgtg caaagagcgc
    31921 ttttttgact tccagacgtt cagacgccgc ttcaataacc agattcgctg ctgccagcgc
    31981 atgaatatcg gtcaccggga tcaggcgttt caatgtgcgt tcacaagttt cagcggtcag
    32041 ttttccccgc ttaacgcgtg aatttagccg cgcgtgtatc ccgtcgattg cgcgggtcag
    32101 cgcttcagca gaaatgtcat acagtaaaac ctgatgtcca tgactggcgg caacttcagc
    32161 aatgcctgcc cccatggtgc cgctcccaat cactgccaca gtttgcacat ttatcatcat
    32221 gctatttccc cgtgaactgc ggtgagcgtt tagccaggaa cgcactgaca ccttcacgat
    32281 aatctgcgct ccgtccggca agtcgctgat agtcgcgttc cagatccagt tgcgtatcga
    32341 gcgtattggt ttcagcgcta tttatcgctt gcttgataag tccaagacca aatgtcggtt
    32401 gtgtcgccag atgccatgcc agctgttgcg cggtatctgc cagcgtttca tcatcaacaa
    32461 cctgccagat catcccccat tcgtgcgcct gttcagcact cagttgattc cccagcagtg
    32521 ccagccccat ggcgcgcgct cgtccggcaa cgcgtggcag taaccaggtt ccaccgcaat
    32581 cgggtactaa gcctaactta ctaaacgcca tgacgaattt tgctgaacgg gcagcaataa
    32641 cgatgtcgca tcccagtgcc agtgttgcgc cagcccctgc cgccacgcca ttgactgcac
    32701 agatcaccgg ttttggcagt tttgccaggc gacgtatcag tgggttatag aaacgttcaa
    32761 ctgacatccc taaatccggt gcggggcctg tgggatcaac gttacgatcg ttaagatcct
    32821 gaccagcaca aaatccgcgc ccggcacctg taagtaacag gcaacggata gtgtcgtcgc
    32881 gctcgacctg tttcagacac tctgccagtt gtgcgtgcat ctcatcatta aaactgttca
    32941 ggcgttccgg gcggttgagc gttagtgtca tcacgccctt ttctacatga ctgaggatga
    33001 attccatcat tagcgtcctt taaagtcggg cgtgcgtttt tgtaagaaag cggagatgcc
    33061 ttcatgacga tcttctgttg ccgccagcaa ggtgaataac tgtcgctcct gggcaagtcc
    33121 ggcttgcaaa gccacttcct gcgactggcg cagcgcttgc tttgccgctt gtaaggccag
    33181 cggcgagtga cgtgccattt tcgacgccag ctgtaaggcg tattcgaggg ttaaatcgct
    33241 ggggaagacg tcgctaacca gcccggcctg ctgtgcttgc tgagcggtga tactttctcc
    33301 gctcagcacc attttgctgg ctaacgattt accgacacta cggattaaac gttgcgttcc
    33361 tcctgcgcct ggcatgatgc cgagagtgat ttccggcaaa ccaaaacgcg cgttctctcc
    33421 ggcaaccacc acatcgcaca acaatgccag ttcgcaaccc gcaccaagcg cgtaaccgtt
    33481 gacggctgcg atgagaggtt tgttgaaggc ctgcaatcgc gcccatagct gcgggcgtgt
    33541 atcgtttaag gtggccgcga gatctttttc tgccatttcg ttgagatcgg ccccagcggc
    33601 aaaaaagcgt gcattaccgg taatcacaca gaccgaaatg ctgctatcgg tagccgcagc
    33661 ttccagttca tttaccagtt gcgtcagaag ggcattattt agcgcattac gtgcggcggg
    33721 acggttaagg gtcagcaaca atacttgttg ctgacggctg acgatcagtt cgctcatgcc
    33781 atccccttcg cgtcaaagtc aaccaccaca tcgctggtca gcggcagtgc ctggcaactc
    33841 aacacatagc ctgcggccag ttcatccggt tccagactgt aattggtttc catcgccact
    33901 ttgccacgca gcactttgca tttgcaggtc gcacagacgc cgcctttgca ggcatagggc
    33961 agatcagccc cctggcgcaa tgccgcatcg agaatgcttt catcgtcggc attaagcacg
    34021 atttcccgat cccgcccatc ctgacgtaca gtcacttttt gtccgtcact ttgcacgtta
    34081 acgctacgtt tgacgcgcgt gccaggcgta ttaaaccgct ccagatgaat cgctttttct
    34141 ggcattccca gtgcttttaa ggcggtttcc gcgtcatcca tcatcgccgc cggcccacaa
    34201 ataaatgcct catcataaag acgaaaatta atgagcgagg ccccaagtga ctgtaatttt
    34261 tcaccgtcaa tacgcccgtg aagcagatcg ctgtcgaggg tttcctgact gaaaatgcac
    34321 aacaactgta aacgctgagg atatttgtct ttcaggtctg ccagtgcctg gcgaaacatc
    34381 atgctctggc tggtacggtt accgtagatt agggtgaact gactttcagg ctcggtttgt
    34441 aaagtggtgg cgataatcgc cagcattggc gtaatacctg atcctgctgc aatagccaga
    34501 tagcgcccct ggcgttcggc ctgcggctga tagccgaaat ggccctgcgg caccatgact
    34561 tccagcgtca tcccctgacg gatgtgatcg cgggcatagc gggaaaaacg tccgccttca
    34621 atggctttta ccgccacact aatttcgcca ggcaggtagc tgcggcaaat ggagtaacaa
    34681 cggcgtaatt cttcaccatc aaggctggct tttaaggtca agtgttgacc ggggcgaaag
    34741 cgatacgcct cctgcaaagg ctggggcacc gcaaaggtaa tggtcaccgc atcacgggtt
    34801 tccggctcca cttttgccac cgttaaggaa tgaaacgttg tcatggcatc ctcaaataca
    34861 tttgaaataa tcgaaaggtt cgcggcaact atcgcagcga tacaatgctt tgcaggccgt
    34921 ggaaccaaat tcactgataa gtgtggtatg gacgctggca cagcgcgggc aacatacttc
    34981 tggcggcaaa tgggcatggc aactgtgtcc ggcgggcggg ctaatgccat actgtcgcag
    35041 acgctcacgg gcatcggggg tcatccagtc ggtggtccat gccgggtcaa gttgcagcac
    35101 aacctgaacg ggggtaaacc cgtgggttga cattgcctca cgtatcgcgc caatcaaatg
    35161 ttccgttgcc ggacaaccgg aatatgtcgg cgtaaagccg atcacccatc cttctcccat
    35221 ctgtgtcaca ttacgcacca tgcctaaatc agtaatggtc agcaccggga tctccgggtc
    35281 cgggatctgg ctgagtagcg cccatatctc atgaacctgt ggcggtgcaa tggtggccag
    35341 acgttgcata cccatctcct ctgttaccat tgctgaccgg gcaagacacg ctggagatac
    35401 tgcatttctg ccagcatggg tccaaggtgt tcggtatgca gtcctttttt accgccagtg
    35461 cgatacgcct gctcttgcgg tacgttcaat gtggcttcgt tgatcccggc aaaaacttcg
    35521 gcttcccacg ctgcgcgtaa agtgcgtgga tcaaccgcaa taccctcttc actcagtgca
    35581 atatcaatct cgtcggcatc gaacagttcg gcggtaaaac gccacaactt gttgatggcc
    35641 tgctgcatct tttgccctga tacgtcagta ccattgccca gtcgctccag ccagccacga
    35701 ctaaaacgca ggtgataacg cgcttcttta attgctttgg cagaaatcgc cgccagttgc
    35761 ggatcacggc tctccatcag acgggtaaag agcgccacat gccatgcatc gatgaaatac
    35821 tggcgtgcaa tggtgtcggc aaaattgccg tttggctgtt caaccaacaa taagttgctg
    35881 aactggcgct cgtctcgggt gaaggccagg gtatcttcat cgccttctcc cgctaattca
    35941 gcggcatacg ataagaagtt gcgtgcctgg cctaataaat cgagtccaat atttgccagt
    36001 gcgagatcga tttccagttc cggtgcatga ccgcaccatt cacccaaccg ctgggagagc
    36061 accaggcagt tatcgcccag gcgcaaggtg taagccgtta actgattcat ttccgacctc
    36121 acatgtgctc aatgccatca gggatggtgt aaaacgttgg atggcgatag accttgcttt
    36181 cagccggatc aaaaaattca ccgcgttctt ccggttgcga ggcaacaatt tcactcgcct
    36241 tcaccaccca aattgaacat ccttcgctac gacgggtgta agcatcacgg gcattttcca
    36301 gtgccatccg ctcatcggca gcatgtaaac tgccaacatg gcggtgtgat aagccctgtt
    36361 tgccacgcac gaaaacttcg tataacggcc agtaaacatt actcattttg aatctcctta
    36421 agcgacctta cgggcaagtt gtttttgtgc atgggccagc gctgcttccc gtacccaggt
    36481 accttcttcc caggctttac gtttggcgtc gagcctctcc tgattacaaa ttccgcgacc
    36541 gttaattact tcgttaaact cctgccagtc gatctcacca aagcggtagt gaccgctttc
    36601 agtgtcaaaa tgcagatccg ggtcaggaac ggccataccg agcatttcaa cctgtggaac
    36661 ggtgttgtcc acgaaacgct ggcggagttc gtcgttggtg aaacgtttga ttttccaggc
    36721 gagacttctg gcgctgtttg gcgagttatc atcgtttggc ccgaacatca ttaaggctgg
    36781 ccaccagaaa cggttaatgg cgtcttgcaa catctgcttt tgagcttcgc tgccctgcgc
    36841 cagtgccatg caggcttcaa acccctgacg ctggtgaaaa ctctcttctt tgcagatttt
    36901 caccattgct ctggcatacg ggccataaga agtgcggcat aacgccacct gattgacgat
    36961 cgctgcgcca tccaccagcc agccgataac gccgatatca gcccaactca atgtcggata
    37021 attaaagatg gaggaatatt tcatccgtcc gtcgagcatt ttttggtaga tgtcttctct
    37081 ggcgcagccc agtgtttcgg cggcgctata gagatacaga ccatgaccgg cttcatcctg
    37141 cactttggcc aacagaatgg ctttacgccg caaggttggt gcgcgagtga tccagttacc
    37201 ttcaggcaac ataccaacaa tctcggagtg cgcatgctgc ccaatctggc ggatcagtgt
    37261 ctttcggtaa gcatcgggca tccagtcctg tggctcgata gccgtttcct gggctatccg
    37321 ttgctcaaag cgttcttctt gggttacgtg atcaccttta cgattccagt tttcgtgaat
    37381 taattaaaaa cacgaatcac attgttttat aaaagttaca caatggttaa ctaaaatcac
    37441 aaattttgta tgcttgaact gtgacggatt ttgcacccta tttttataaa ccattattta
    37501 tcagtgcatt aatgttttcc gcagttgcta tacagatcgc atagttaaca tttcgttaaa
    37561 agaccccttg ctttttatga ttcgcgattt aactattagc aacagaaatg tgaaacatct
    37621 ggagagtagc gatgcagcag ttagccagtt tcttatccgg tacctggcag tctggccggg
    37681 gccgtagccg tttgattcac cacgctatta gcggcgaggc gttatgggaa gtgaccagtg
    37741 aaggtcttga tatggcggct gcccgccagt ttgccattga aaaaggtgcc cccgccctcc
    37801 gcgcgatgac ctttatcgaa cgtgcggcga tgcttaaagc ggtcgctaaa catctgctga
    37861 gtgaaaaaga gcgcttctat gctctttctg cgcaaacagg cgcaacgcgg gcagacagtt
    37921 gggttgatat tgaaggtggc attgggacgt tatttactta cgccagcctc ggtagccggg
    37981 agctgcctga cgatacgctg tggccggaag atgaattgat ccccttatcg aaagaaggtg
    38041 gatttgccgc gcgccatgta ctgacctcaa agtcaggcgt ggcagtgcat attaacgcct
    38101 ttaacttccc ctgctgggga atgctggaaa agctggcacc aacgtggctg ggcggaatgc
    38161 cagccatcat caaaccagct accgcgacgg cccaactgac tcaggcgatg gtgaaatcaa
    38221 ttgtcgatag tggtcttgtt cccgaaggcg caattagtct gatctgcggt agtgctggcg
    38281 acttgttgga tcatctggac agccaggatg tggtgacttt cacggggtca gcggcgaccg
    38341 gacagatgct gcgagttcag ccaaatatcg tcgccaaatc tatccccttc actatggaag
    38401 ctgattccct gaactgctgc gtactgggcg aagatgtcac cccggatcaa ccggagtttg
    38461 cgctgtttat tcgtgaagtt gtgcgtgaga tgaccacaaa agccgggcaa aaatgtacgg
    38521 caatccggcg gattattgtg ccgcaggcat tggttaatgc tgtcagtgat gctctggttg
    38581 cgcgattaca gaaagtcgtg gtcggtgatc ctgctcagga aggcgtgaaa atgggcgcac
    38641 tggtaaatgc tgagcagcgt gccgatgtgc aggaaaaagt gaacatattg ctggctgcag
    38701 gatgcgagat tcgcctcggt ggtcaggcgg atttatctgc tgcgggtgcc ttcttcccgc
    38761 caaccttatt gtactgtccg cagccggatg aaacaccggc ggtacatgca acagaagcct
    38821 ttggccctgt cgcaacgcta atgccagcac aaaaccagca acatgctctg caactggctt
    38881 gtgcaggcgg cggtagcctt gcgggaacgc tggtgacggc tgatccgcaa attgcgcgtc
    38941 agtttattgc cgacgcggca cgtacgcatg ggcgaattca gatcctcaat gaagaatcgg
    39001 caaaagaatc caccgggcat ggctccccac tgccacaact gttacatggt gggcctggtc
    39061 gcgcaggagg cggtgaagaa ttaggtggtt tacgagcggt gaaacattac atgcagcgaa
    39121 ccgctgttca gggtagtccg acgatgcttg ccgctatcag taaacagtgg gtgcgcggtg
    39181 cgaaagtcga agaagatcgt attcatccgt tccgcaaata ttttgaggag ctgcaaccag
    39241 gcgacagcct gttgactccc cgccgcacaa tgacagaggc cgatattgtt aactttgctt
    39301 gcctcagcgg cgatcatttc tatgcacata tggataaaat tgctgctgcc gaatctattt
    39361 ttggtgagcg tgtggtacat ggatattttg tgctttctgc ggctgcgggt ctgtttgtcg
    39421 atgccggtgt cggtccggtc atagctaact acgggctgga aaacttgcgt tttatcgaac
    39481 ccgtaaaacc aggcgatacc atccaggtgc gtctcacctg taagcgcaag acgctgaaaa
    39541 aacagcgtag cgcagaagaa aaaccaacag gtgtggtgga atgggcggta gaggtattca
    39601 atcagcatca aaccccggtg gcgctgtatt caattctgac gctggtggcc aggcagcacg
    39661 gtgattttgt cgattaatcg gtgaatgaag ggcaacggcg aatagttgcc cttttatttc
    39721 actaaatttt gtgacgttgt cacattatgc atgatgtgta catctatttt cagggcatcc
    39781 actgtatgaa aagctggcac acctgccaaa caacctggca ggtgcaggca atcccctttg
    39841 catcagtact gataatgtga acctgactaa accgcccaca gagcgcggtt gctaacaaga
    39901 acacaacatc tgacgaggtt aataatggga agcccctctc tgtattctgc ccgtaaaaca
    39961 accctggcgt tggcagtcgc cttaagtttc gcctggcaag cgccggtatt tgcccacggt
    40021 ggtgaagcgc atatggtgcc aatggataaa acgcttaaag aatttggtgc cgatgtgcag
    40081 tgggacgact acgcccagct ctttaccctg attaaagatg gcgcgtacgt gaaagtgaag
    40141 cctggtgcgc aaacagcaat tgttaatggt cagcctctgg cactgcaagt accggtagtg
    40201 atgaaagaca ataaagcctg ggtttctgac acctttatta acgatgtttt ccagtccggg
    40261 ctggatcaaa cctttcaggt agaaaagcgc cctcacccac ttaatgcgct aactgcggac
    40321 gaaattaaac aggccgttga aattgttaaa gcttccgcgg acttcaaacc caatacccgt
    40381 tttactgaga tctccctgct accgccagat aaagaagctg tctgggcgtt tgcgctggaa
    40441 aacaaaccgg ttgaccagcc gcgcaaagcc gacgtcatta tgctcgacgg caaacatatc
    40501 atcgaagcgg tggtggatct gcaaaacaac aaactgctct cctggcaacc cattaaagac
    40561 gcccacggta tggtgttgct ggatgatttc gccagtgtgc agaacattat taacaacagt
    40621 gaagaatttg ccgctgccgt gaagaaacgc ggtattactg atgcgaaaaa agtgattacc
    40681 acgccgctga ccgtaggtta tttcgatggt aaagatggcc tgaaacaaga tgcccggttg
    40741 ctcaaagtca tcagctatct tgatgtcggt gatggcaact actgggcaca tcccatcgaa
    40801 aacctggtgg cggtcgttga tttagaacag aaaaaaatcg ttaagattga agaaggtccg
    40861 gtagttccgg tgccaatgac cgcacgccca tttgatggcc gtgaccgcgt tgctccggca
    40921 gttaagccta tgcaaatcat tgagcctgaa ggtaaaaatt acaccattac tggcgatatg
    40981 attcactggc ggaactggga ttttcacctc agcatgaact ctcgcgtcgg gccgatgatc
    41041 tccaccgtga cttataacga caatggcacc aaacgcaaag tcatgtacga aggttctctc
    41101 ggcggcatga ttgtgcctta cggtgatcct gatattggct ggtactttaa agcgtatctg
    41161 gactctggtg actacggtat gggcacgcta acctcaccaa ttgctcgtgg taaagatgcc
    41221 ccgtctaacg cagtgctcct taatgaaacc atcgccgact acactggcgt gccgatggag
    41281 atccctcgcg ctatcgcggt atttgaacgt tatgccgggc cggagtataa gcatcaggat
    41341 atgggccagc ccaacgtcag taccgaacgc cgggagttag tggtgcgctg gatcagtaca
    41401 gtgggtaact atgactacat ttttgactgg atcttccatg aaaacggcac tattggcatt
    41461 gatgccggtg ctacgggcat cgaagcggtg aaaggtgtta aagcgaaaac catgcacgat
    41521 gagacggcga aagatgacac gcgctacggc acgcttatcg atcacaatat cgtgggtact
    41581 acacaccaac atatttataa tttccgcctc gatctggatg tagatggcga gaataacagc
    41641 ctggtggcga tggacccagt ggtaaaaccg aatactgccg gtggcccacg caccagtacc
    41701 atgcaagtta atcagtacaa catcggcaat gaacaggatg ccgcgcagaa gtttgatccg
    41761 ggcacgattc gtctgttgag taacccgaac aaagagaacc gcatggtcaa tccagtttcc
    41821 tatcaaatta ttccttatgc aggtggtact cacccggtag caaaaggtgc ccagttcgcg
    41881 ccggacgagt ggatctatca tcgtttaagc tttatggaca agcagctttg ggtaacgcgt
    41941 tatcatcctg gcgagcgttt cccggaaggc aaatatccga accgttctac tcatgacacc
    42001 ggtcttggac aatacagtaa agataacgag tcgctggaca acaccgacgc cgttgtctgg
    42061 atgaccaccg gcaccacaca tgtggcccgc gccgaagagt ggccgattat gccgaccgaa
    42121 tgggtacata ctctgcttaa accatggaac ttctttgacg aaacgccaac gctaggggcg
    42181 ctgaagaaag ataagtgatt gtttcagaaa aaaaacggca ccaggtgccg ttttttactt
    42241 atgagcgaac cagattaata ccgtacacac accgacttag tttcacacca accgtccagc
    42301 cagtcggggc caaaatcacg gcccgttcct gactgcttca tcccaccaaa cggtaagtta
    42361 gcgtcaatta aggtatggct gtttacccac accgtccctg cctgtaagcg atcgctatat
    42421 tccagagcct gggagagatt ttgcgtccag acactggcag ttaagccata ttccgtgtcg
    42481 tttgccagtt gtaacgcctc ttctccatcc gctactcgca ccaggtttac caccggacca
    42541 aacacctctt cacgagttaa gcgcaattta gcatcgggat ttaccaccag cgttggcgca
    42601 acataatacc cctctccggc tggtccattc gacccgcgaa tcagctctgc ttgctgtgcc
    42661 tgcgcatcgt cgaggaatga acacactttg tcgcagtgcg cacgagaaac caaagggtta
    42721 atctgtgcaa caggtgacat ccccggtccc acttgcaacg attttaccgc ctgctcaaat
    42781 ccactaacca gcgtgtcaaa caacggcgct tcaatataaa ttcgcgaact ggcggcgcat
    42841 acttgccctt gattcaggaa gcttccggtc atcaagcctt caataaccca ttgcggatca
    42901 gcatctttta atacaattgc cgggttttta ccgcccagtt ccagcgttac acgcgttaag
    42961 tgatcagcag cagttctggc aatacctttt cccgtcgcgg ttgaaccggt aaaactgatt
    43021 ttcgcaacat gaagatgtga cgtcagggcc gcgccgcata cagcacctga cccggtgacc
    43081 acattaaaaa cgccatcagg gataccagcc tcgctggcca gttccgccac gcgcaacatc
    43141 gtcagtggcg tggtttccga aggcttaatc acgattgaac agcctgctgc cagtgctggc
    43201 atcaccttcc acatgccaat catcaacgga aagttccatg gcacaattcc cgctaccacg
    43261 ccaaccggct ctttacgcgt ccaggcctga taacgcgccc cctggggtaa gggaatcgac
    43321 aagtccagcg ttttacccgc gattttggtc gttaacccgg cggtataacg catccagttc
    43381 agcgtacagc ccacttcaaa agcacgggaa atggcaattg acttgccttg ctccagggtt
    43441 tccagttgcg ccagctcctc actgtgctgc tccaccagat cagcaaaacg tagcaggata
    43501 cgttcacgct ctgcgggtaa tcgcccggcc cagcgacgcg agacaaaggc ccgccatgca
    43561 gacatgactg cgttatctac atccgcttcg ttggcatcag cagttgatgc aatttcttgc
    43621 ccggttgccg gatcaaagat cgccaaccgt ttttcacttt gtgcggggcc aggacgacca
    43681 tcaatataaa gaccgtgttg acgatcgaga aactgttgga cctggcttaa tactgctaca
    43741 tgcggctctg tcatcacttt tccttattat ttacccagtg tgatttcagt gtacgcagcg
    43801 acaagataag tcactttttt cgctgtgaca ttcgctttgt cagctgtgac aagctccgca
    43861 aatcgtgaca ataaccgctt atgtctctat gcctgacaag acttatttcc tatagtcagg
    43921 ctcattagct tcgattatcg caacacaaat gcaacaataa aaacacattt cacagagcga
    43981 aaacgtgcca tgaaccccgc agtggataat gagtttcagc aatggctttc ccaaatcaat
    44041 caggtatgcg gaaattttac cggacgcctg ctgactgagc gttacacggg tgtactggac
    44101 acgcattttg ccaaaggact aaagctgagt accgtgacaa ccaacggggt gaatttatcc
    44161 cgcacctggc aggaaataaa aggcagcgac gatgcctggt tttacaccgt ttttcagctt
    44221 agtggtcagg caataatgga gcaggatgag cgtcaggtgc agattggcgc tggcgatatt
    44281 acgttactcg atgcctcacg cccctgttcg ctttactggc aggagtcttc taaacagatt
    44341 tcattacttt tgccacgcac tctgctggaa caatattttc cccatcaaaa acctgtctgc
    44401 gcagaaagac tggacgctga cttacccatg gtgcaactca gtcatcgcct gttacaggag
    44461 agcatgaata atccggcact ttctgaaaca gaaagtgaag ctgcgctaca ggcgatggtg
    44521 tgtctgcttc gcccggtact tcatcagcgg gaatctgtcc aacctcgccg tgaacgtcag
    44581 tttcaaaaag tggttacgtt gatagacgat aatattcgcg aagagatatt acgcccggag
    44641 tggatagccg gagagacagg tatgtcagta cgtagtttgt accgaatgtt tgccgataaa
    44701 ggtttggtag tcgcgcaata tattcgtaac cgtcgtctcg atttttgtgc agatgcgatt
    44761 cgccatgccg cagatgatga aaaactggca ggcatcggct ttcattgggg attttctgac
    44821 cagagccatt tttcaacggt atttaagcaa cgctttggga tgacgccagg cgagtatgga
    44881 cgtaaattcc gctaactaat tcgcgttgtg tgtactgcaa cgccaattag ttaaaacaga
    44941 tagttgacta tgcatttttc agggcgaaag gcaatttgac aggagttttt gaatattcac
    45001 cttgtttcag gaaaatagat caatcgcttt ctcctgtagc gttatcccga tattgcggtt
    45061 ttgctattaa aattttcgca cccatttacc gtttatccct tgtacatatt cccccggttt
    45121 ggcccgagct acgagctttt gcccggcgag tttcgcgata tcatctaccg acacattatt
    45181 ttgctttgcc agttgttggt aactcgcttt acgttcggca ttaatttcgg ttactaattt
    45241 ctctgtttca gcatccgttt tcagcgcaac cagataacca taaaatgttt cacctacccg
    45301 cccctgagtt ctggcttcat ccagagtcaa tgccattaca ttgctgctta ccagcccaac
    45361 aagaaacgca caaagaagta atgttttctt catcatcgcc tcagaaaaga tcgctacgag
    45421 tttcaagcag ctcttcgaca tctttgtctg ccttgatgat gatctcatgc tcaattttaa
    45481 cgttcatatt gatagtgatc ggttccttag gtgcagcgac ttcaatgcga ggcgtacagc
    45541 caaccagcat aaaagatgac gtcaacgcag ccagtaaaat tttcattgtt tttcctcaca
    45601 ctcttttcct tgcggacagt catttcccgg cagacgtgcg ttctgctcca accatgcctg
    45661 gagattatcg ccaaagcgta aactgcgcca cagcgtaaaa atattctctt catgatagta
    45721 attaaggttt accgtaccgc ttttaccatc aacgcgactg gtacctgtaa tgttggcctg
    45781 catggttaat aaaccgagat tatctaagtt aatttttgtc gatgaccggc taatttccat
    45841 atagcgcaac cagttaattg ctgaacccgc agtcatattg tctttcacca ccgcatctgc
    45901 ggtgtcttta tccagccgca aggtcattgg cccgctattt gccagccaac catctttcac
    45961 tatccatttt tcgttgttta gccataacgg caatgcacca ctaaatgccc cggacatggc
    46021 gaattgtttc ggattgacgg cgctaaccag ttcgctggat gagagattat tcagccgcaa
    46081 cagcgccggg tcatgctgcg gcatacgtaa ttgtttcatc agtacgttac cgcctaacac
    46141 atcgacgcta acatcagtca acagcaatgg ttcttcttca gtccacggat aacgcccttg
    46201 caaatcagcc gtaatatttt tcgctgtcac cagattaatc acttcggcaa ttcgcaacgt
    46261 aacggggccg cgagtcccca gatgccaggc tccatcggca aaacggaaag gcaggacaaa
    46321 atcgacaccg ttaacctgat tatctggcat ccaggcacta ccgcctttca acacgccgtg
    46381 ccctcccgcg cggaatcctt gttcaggcgc agcagaaaat gcaacctgag catacagttc
    46441 accatcgcgt aagttcatct tccagtcggg tggcaccagc ggctggaata cagtcagtga
    46501 ttgtttaggc caccaggcgt taccgcgcag acgaataccg tcccagcgac catttacccg
    46561 aaccgggcca atctcaccag catgtaaatc gcctttaaag aggaaatacg tcggatcgcg
    46621 cccatcgacg ctaaatttta aggttgatgg cggtaacaca ctgccgccag tgaacagcgt
    46681 ttgcccggcg tccagtgaca gcgcgccgct gaaggaggga tgctgtgcgt cacgtaccca
    46741 gacgacgggc ttgtcgagaa ttaatcgcgg cttttctacc gtcatcgtac cgtattgtaa
    46801 ctggtcgaaa ccggtggaga gatcggtcag cgtaatcgtg ctgtcatgcc actcaccttt
    46861 tcctgcgaca tcccaggtgg cattcatcgg tgtaaaactc ccttttcccc agtagcgcca
    46921 ctgccagcgg ccagcgtcag ggagaaaatc attcgccagc ccatccatat gcagcacgaa
    46981 atcgcccagt tcattttcat gcgcctgcaa gatggcctgc aaacgtccgt caacaccacg
    47041 ttgggtgact tttacacccg ctaaaggcca gcggatttca tcgatgtcca gcgaatcgat
    47101 gactcttccc ttcgaacgta ataacgcgcc tggctcaaag gccaacgttg ggtcagtcag
    47161 acttccgctt agctgcgcag gtaaacgggc atataaaatg aggtccgcct gtttcgcttc
    47221 accggtcagt tgcagaggca gctgactgtt atccatgctt aattttcctg ggccaaaatt
    47281 aagcaccgcg ttgcctttac ccgcttgccc ctgggtcagc acgctcagtc gtccgctgac
    47341 cagagcgttc tcgagccctg cctgccagtt gtcgacttta acacccagtc ggccactcaa
    47401 aggaaaacct gcatacggcc agctccagcg accatcgctt acggtcaatt gttgacgagt
    47461 aatttgccac ggcaaatcga gcaacggatc gccgttatcc cgtgccaaca cgatcaattg
    47521 cccgctattt tcctgccagt ccagctcggc atccaccagt gaaggctcct gcggcaagtt
    47581 taacgtcgca gtagcatgac cacttacagg aagtccatcc ggcactagcg gcatagtaaa
    47641 ttcccccacc agtttaaccg gcggctgatt ttcaaacgcg acgacatcca gttcgctgac
    47701 tgtaagttgt tgccctttca gctgaccttg aaatttaact ttttcgccct gataacgcag
    47761 ttgctggata tcagaggtta atgcgagaga gagttttccc tgccattcct gccagggaga
    47821 aaaaatcagt ttatcgatat tgatccaggt gttaggcagc atggactgcc attgcgcgag
    47881 ggtttttgga gcggctggcg attgctccgt ctgcggcaat ttcgccagac aagcagaatc
    47941 aagttctacc gtgccgacgt tcaataacca gcggctagga tgtgaaaggc tggcgttggt
    48001 gatatgcgca agctgacaat ctcccaccag ataacggaga tcgggaatga ttaaaccttt
    48061 acgcgtaatg cgtgggcttt catctaatgc aatacgtgtc ccgagcggta gccagatgcc
    48121 cgccagcgtg ggaacccaca gcccgagcgt catcagcagc gtcaacggca caagaataat
    48181 cagtaataac agcgcgagaa cggctttata tttacccagc atgggtagtt aatatcctga
    48241 tttagcgaaa aattaagcat tcaatacggg tattgtggca tgtttaaccg ttcagttgaa
    48301 ggttgcgcct acactaagca tagttgttga tgaatttttc
//
LOCUS       DAZFAH010000013        47723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFAH010000013 DAZFAH010000000
VERSION     DAZFAH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.013"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..687
                     /gene="comR"
                     /locus_tag="ACHJWB_000701"
     CDS             55..687
                     /gene="comR"
                     /locus_tag="ACHJWB_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4312381.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLHLWPELVK"
     gene            complement(749..1288)
                     /locus_tag="ACHJWB_000702"
     CDS             complement(749..1288)
                     /locus_tag="ACHJWB_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4312382.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1515..2819)
                     /gene="ndh"
                     /locus_tag="ACHJWB_000703"
     CDS             complement(1515..2819)
                     /gene="ndh"
                     /locus_tag="ACHJWB_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4312383.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3219..3761)
                     /gene="ycfP"
                     /locus_tag="ACHJWB_000704"
     CDS             complement(3219..3761)
                     /gene="ycfP"
                     /locus_tag="ACHJWB_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4312384.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3784..4809)
                     /gene="nagZ"
                     /locus_tag="ACHJWB_000705"
     CDS             complement(3784..4809)
                     /gene="nagZ"
                     /locus_tag="ACHJWB_000705"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4312385.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKTISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4820..5644)
                     /gene="thiK"
                     /locus_tag="ACHJWB_000706"
     CDS             complement(4820..5644)
                     /gene="thiK"
                     /locus_tag="ACHJWB_000706"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4312386.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5625..6266)
                     /gene="lpoB"
                     /locus_tag="ACHJWB_000707"
     CDS             complement(5625..6266)
                     /gene="lpoB"
                     /locus_tag="ACHJWB_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4312387.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6280..6657)
                     /locus_tag="ACHJWB_000708"
     CDS             complement(6280..6657)
                     /locus_tag="ACHJWB_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4312388.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6660..7019)
                     /gene="hinT"
                     /locus_tag="ACHJWB_000709"
     CDS             complement(6660..7019)
                     /gene="hinT"
                     /locus_tag="ACHJWB_000709"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4312389.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7353..9542
                     /gene="fhuE"
                     /locus_tag="ACHJWB_000710"
     CDS             7353..9542
                     /gene="fhuE"
                     /locus_tag="ACHJWB_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4312390.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(9602..11035)
                     /gene="ptsG"
                     /locus_tag="ACHJWB_000711"
     CDS             complement(9602..11035)
                     /gene="ptsG"
                     /locus_tag="ACHJWB_000711"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4312391.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11330..12127)
                     /locus_tag="ACHJWB_000712"
     CDS             complement(11330..12127)
                     /locus_tag="ACHJWB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4312392.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12138..13142)
                     /gene="holB"
                     /locus_tag="ACHJWB_000713"
     CDS             complement(12138..13142)
                     /gene="holB"
                     /locus_tag="ACHJWB_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4312393.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13139..13780)
                     /gene="tmk"
                     /locus_tag="ACHJWB_000714"
     CDS             complement(13139..13780)
                     /gene="tmk"
                     /locus_tag="ACHJWB_000714"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4312394.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13770..14792)
                     /gene="yceG"
                     /locus_tag="ACHJWB_000715"
     CDS             complement(13770..14792)
                     /gene="yceG"
                     /locus_tag="ACHJWB_000715"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4312395.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14795..15604)
                     /gene="pabC"
                     /locus_tag="ACHJWB_000716"
     CDS             complement(14795..15604)
                     /gene="pabC"
                     /locus_tag="ACHJWB_000716"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4312396.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPAKR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15724..16965)
                     /gene="fabF"
                     /locus_tag="ACHJWB_000717"
     CDS             complement(15724..16965)
                     /gene="fabF"
                     /locus_tag="ACHJWB_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4312397.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17053..17289)
                     /gene="acpP"
                     /locus_tag="ACHJWB_000718"
     CDS             complement(17053..17289)
                     /gene="acpP"
                     /locus_tag="ACHJWB_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4312398.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17500..18234)
                     /gene="fabG"
                     /locus_tag="ACHJWB_000719"
     CDS             complement(17500..18234)
                     /gene="fabG"
                     /locus_tag="ACHJWB_000719"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4312399.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQS
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18247..19176)
                     /gene="fabD"
                     /locus_tag="ACHJWB_000720"
     CDS             complement(18247..19176)
                     /gene="fabD"
                     /locus_tag="ACHJWB_000720"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4312400.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19192..20145)
                     /gene="fabH"
                     /locus_tag="ACHJWB_000721"
     CDS             complement(19192..20145)
                     /gene="fabH"
                     /locus_tag="ACHJWB_000721"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4312401.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20213..21283)
                     /gene="plsX"
                     /locus_tag="ACHJWB_000722"
     CDS             complement(20213..21283)
                     /gene="plsX"
                     /locus_tag="ACHJWB_000722"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4312402.1"
                     /translation="MTCLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSHAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21364..21537)
                     /gene="rpmF"
                     /locus_tag="ACHJWB_000723"
     CDS             complement(21364..21537)
                     /gene="rpmF"
                     /locus_tag="ACHJWB_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4312403.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21589..22110)
                     /gene="yceD"
                     /locus_tag="ACHJWB_000724"
     CDS             complement(21589..22110)
                     /gene="yceD"
                     /locus_tag="ACHJWB_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4312404.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22309..22893
                     /gene="yceF"
                     /locus_tag="ACHJWB_000725"
     CDS             22309..22893
                     /gene="yceF"
                     /locus_tag="ACHJWB_000725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4312405.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJWB_000726"
     CDS             complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJWB_000726"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4312406.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24537..27722
                     /gene="rne"
                     /locus_tag="ACHJWB_000727"
     CDS             24537..27722
                     /gene="rne"
                     /locus_tag="ACHJWB_000727"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4312407.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHJWB_000728"
     CDS             complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHJWB_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4312408.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHJWB_000729"
     CDS             complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHJWB_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4312409.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHJWB_000730"
     CDS             complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHJWB_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4312410.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHJWB_000731"
     CDS             complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHJWB_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4312411.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHJWB_000732"
     CDS             complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHJWB_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4312412.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33394..34176)
                     /gene="flgG"
                     /locus_tag="ACHJWB_000733"
     CDS             complement(33394..34176)
                     /gene="flgG"
                     /locus_tag="ACHJWB_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4312413.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34314..35069)
                     /gene="flgF"
                     /locus_tag="ACHJWB_000734"
     CDS             complement(34314..35069)
                     /gene="flgF"
                     /locus_tag="ACHJWB_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4312414.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35089..36294)
                     /gene="flgE"
                     /locus_tag="ACHJWB_000735"
     CDS             complement(35089..36294)
                     /gene="flgE"
                     /locus_tag="ACHJWB_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4312415.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKTGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36319..37014)
                     /gene="flgD"
                     /locus_tag="ACHJWB_000736"
     CDS             complement(36319..37014)
                     /gene="flgD"
                     /locus_tag="ACHJWB_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4312416.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37026..37430)
                     /gene="flgC"
                     /locus_tag="ACHJWB_000737"
     CDS             complement(37026..37430)
                     /gene="flgC"
                     /locus_tag="ACHJWB_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4312417.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37434..37850)
                     /gene="flgB"
                     /locus_tag="ACHJWB_000738"
     CDS             complement(37434..37850)
                     /gene="flgB"
                     /locus_tag="ACHJWB_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4312418.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38006..38665
                     /gene="flgA"
                     /locus_tag="ACHJWB_000739"
     CDS             38006..38665
                     /gene="flgA"
                     /locus_tag="ACHJWB_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4312419.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38741..39034
                     /gene="flgM"
                     /locus_tag="ACHJWB_000740"
     CDS             38741..39034
                     /gene="flgM"
                     /locus_tag="ACHJWB_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4312420.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39039..39455
                     /gene="flgN"
                     /locus_tag="ACHJWB_000741"
     CDS             39039..39455
                     /gene="flgN"
                     /locus_tag="ACHJWB_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4312421.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKSQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39495..41030)
                     /gene="murJ"
                     /locus_tag="ACHJWB_000742"
     CDS             complement(39495..41030)
                     /gene="murJ"
                     /locus_tag="ACHJWB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4312422.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41140..42063)
                     /locus_tag="ACHJWB_000743"
     CDS             complement(41140..42063)
                     /locus_tag="ACHJWB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4312423.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFARLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42065..42712)
                     /locus_tag="ACHJWB_000744"
     CDS             complement(42065..42712)
                     /locus_tag="ACHJWB_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4312424.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42723..43307)
                     /gene="rimJ"
                     /locus_tag="ACHJWB_000745"
     CDS             complement(42723..43307)
                     /gene="rimJ"
                     /locus_tag="ACHJWB_000745"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4312425.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43543..44751
                     /gene="mdtH"
                     /locus_tag="ACHJWB_000746"
     CDS             43543..44751
                     /gene="mdtH"
                     /locus_tag="ACHJWB_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4312426.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44815..45462
                     /gene="grxB"
                     /locus_tag="ACHJWB_000747"
     CDS             44815..45462
                     /gene="grxB"
                     /locus_tag="ACHJWB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4312427.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45596..46156
                     /locus_tag="ACHJWB_000748"
     CDS             45596..46156
                     /locus_tag="ACHJWB_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4312428.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46262..47308
                     /gene="pyrC"
                     /locus_tag="ACHJWB_000749"
     CDS             46262..47308
                     /gene="pyrC"
                     /locus_tag="ACHJWB_000749"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4312429.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47382..47627
                     /gene="dinI"
                     /locus_tag="ACHJWB_000750"
     CDS             47382..47627
                     /gene="dinI"
                     /locus_tag="ACHJWB_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4312430.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11541 a        12921 c        11847 g        11414 t
ORIGIN      
        1 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca
       61 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
      121 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
      181 gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
      241 aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat
      301 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
      361 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
      421 tgcaccaccc tctccccaga ttcaggagat atcgccaata cgttgaaatc acgccatgcg
      481 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
      541 acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg
      601 atcagcgcac gcgaaggtgc atcgctggaa aaactaatgc agattgccgg aacgactttg
      661 catttatggc ccgaactggt gaaataagtc gcaaaaaaag cccctcagcc tgcgcgaatg
      721 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccat
      781 tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat
      841 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
      901 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
      961 taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta ccgcgaccac
     1021 cgccaaactg atgccctatc acgccgccag caacagcgcc gagcaccgac ccggtaatgc
     1081 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
     1141 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggccac
     1201 gttcaaacac gttcagactg gccactgccg ctacgcccag cgcagctgcg acaccaatcc
     1261 cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat
     1321 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatggctg
     1381 gaaatactta atgattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
     1441 tatcaggcct accgctcgta ccatttgtcc agggcggatg aggcgtttat gccacatccg
     1501 ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc
     1561 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
     1621 agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
     1681 ttacccatca ggctaccaac ggtggagaag ttcgacagcg ataccagcga accatgatct
     1741 ttatactgat aatttttcag cggcttacca ttcatctgcg ccagaatgtt gttcattgcg
     1801 caagtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc
     1861 gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgggtggt ttgcagcgtc
     1921 ggttccacca ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag
     1981 tctggcgctt tgatcccggc tgcccacacc atcagatcag cctcaatata ttcgccatct
     2041 ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg
     2101 ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa
     2161 atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa
     2221 ccgtagctgt gcagttgctt gaccgcgttg tgcaattcag cggagagttc tacacccgtc
     2281 gcgccgccgc cgacaatcgc aatgttcact ttgccatttg cgcccaggtt ggcagagtat
     2341 ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
     2401 aaaatgcagt tctctttgac gcctggcgta ttgaaatcgt tagaggtgct acccagcgcc
     2461 atgaccaggg tgtcataggc gattttacgt tccggaacca gcagttcacc tttctcgtcg
     2521 cgtagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccaac
     2581 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
     2641 tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc acaggtggct gtggttacga
     2701 tcgaccagcg taattttggc ttttttctta cgtcccagct tatgccccaa ctgtgttgcc
     2761 atttccagcc caccagcacc gccgccgaca atcacaatct ttttcaatgg cgtagtcaac
     2821 gtgaccccct taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat
     2881 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
     2941 ttggtcatac caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac
     3001 cgggtgagat caaaaaatag agtttattgt ttattatttg agcaaattcg gaagagtaat
     3061 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagaggcgc atcaggcatt
     3121 gatgctcgac tgtcggatgc ggcgtaaacg ccttatccga cccacagaat ccggagcgca
     3181 tcaggtaccg gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct
     3241 ttaatgcgct gtaaatgcgg ggagatattc ttaaatttgt gcgtctgctc ttcgtcccag
     3301 acaatctcgt aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca
     3361 ttacgcgaca aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac
     3421 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
     3481 gggaacaaat tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc
     3541 gcccagtatc cacccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc
     3601 aacattttgt ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg
     3661 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
     3721 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
     3781 gggttagtga cctgctttct cttcctgcca gcgttcgtgt aactgattca gacgggtgct
     3841 gatcgttttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
     3901 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
     3961 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
     4021 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
     4081 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
     4141 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
     4201 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
     4261 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
     4321 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
     4381 cagggctttt tgtggatcgg catgatatga acgctcgcca atcgccgcgc tgatatgccc
     4441 gacatccagc actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
     4501 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa
     4561 ggattgtgct gccggtaagc gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc
     4621 ctgatctacc gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc
     4681 acgtaactgg gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg
     4741 atgcgccagt atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac
     4801 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
     4861 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga
     4921 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
     4981 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca
     5041 ccgccgccag ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta
     5101 accctgacgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
     5161 gtaaaggccg tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg
     5221 tccgccgcgc cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc
     5281 gccagccaaa acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
     5341 ccggcaaata cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat
     5401 ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca
     5461 agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct
     5521 gatgttcaat gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa
     5581 actgcgggaa aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg
     5641 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
     5701 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
     5761 cgggcaatgc ctatggcttt actacgggta cctaaactgt cctgcggcga caaacctaac
     5821 tgttgcttag ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc
     5881 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
     5941 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
     6001 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
     6061 gccgtttgat cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc
     6121 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
     6181 accggtgcag gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc
     6241 aaggcgtagc gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct
     6301 gactttgtgc gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg
     6361 aatgctaacg ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca
     6421 gtaaaaacga taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc
     6481 tgaaggttga atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa
     6541 taaagatgac tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg
     6601 acagcccacc agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt
     6661 tacagacctt tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg
     6721 atgtggtaaa cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct
     6781 tcggcaatac cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc
     6841 tgctcatgct cagctgagac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga
     6901 atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag
     6961 acgatatcgg aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg
     7021 acattttcct ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag
     7081 tatagagtat ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa
     7141 aaaatcgttt tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg
     7201 aaaagacatt ctctgtttgg tttacacgac aaatatgaat gcgtatattt ctcatttgca
     7261 tttacaaaca aaattattcg cacactaaat ataatcgctc atcaccctcc tccggcatga
     7321 gcctgacgac aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata
     7381 atcaatatca agccatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat
     7441 taccttctgc cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag
     7501 ccacagctcc agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca
     7561 aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga
     7621 tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca
     7681 aaagtcaggc ggattccgat cgtgctcttt attattcccg cggattccag atcgataact
     7741 atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt
     7801 ctgatatggc actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg
     7861 ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat
     7921 ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt
     7981 tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga
     8041 ataacgactc atggctggac cgctacaaca gtgaaaagac cttcttctcg ggcattgtcg
     8101 atgctgattt aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg
     8161 ttaatagccc tacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt
     8221 acgatcgtgc acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg
     8281 tctttatgac cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc
     8341 actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg
     8401 atggtatgct ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca
     8461 ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtattt
     8521 atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca
     8581 atcgttactt cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact
     8641 ttaatggcaa tttcccacaa accgactggt caccacagag cctggcacag gacgatacca
     8701 cacatatgaa atcgttatat gctgccactc gagtcaccct tgccgatccg ctgcatctga
     8761 tcctcggcgc acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa
     8821 accacaccac gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg
     8881 ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg
     8941 ctccaatcac cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc
     9001 tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta
     9061 cacctatccc cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta
     9121 aaggggtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg
     9181 caacgcgcta tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca
     9241 ccacggttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg
     9301 gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc
     9361 gcgccgagca aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa
     9421 acttctcgtt acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg
     9481 aaggttctat cgtctacggc acaccgcgta atttcagcat taccggcacg tatcaattct
     9541 gaataacacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg
     9601 attagtggtt acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga
     9661 aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc
     9721 cggcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt
     9781 cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc
     9841 tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca
     9901 gtgctttaat cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga
     9961 tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt
    10021 gcgagaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg
    10081 ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg
    10141 gctcggtgat accggtcagg aacgaggtca gcgccgcgga aatcataata ccgcccactt
    10201 tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt
    10261 acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac
    10321 gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct
    10381 ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac
    10441 cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga
    10501 ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca
    10561 ggccagaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct
    10621 taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga
    10681 gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca
    10741 gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg
    10801 cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca
    10861 tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc
    10921 agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg
    10981 gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg
    11041 agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc
    11101 ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag
    11161 tgccaatttg tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt
    11221 ttgacaaaac atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg
    11281 tgaactttac tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg
    11341 attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg
    11401 ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca
    11461 tcgcaggttg attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt
    11521 ccaccagtaa tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac
    11581 ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg
    11641 tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc
    11701 gcagaattgc cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca
    11761 gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg
    11821 gtgtgtaata ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca
    11881 gacggcgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag
    11941 aaaatacgac gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg
    12001 ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat
    12061 ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta
    12121 aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa
    12181 tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac
    12241 attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa
    12301 agatggtttg ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg
    12361 ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga
    12421 gcttgttcat gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat
    12481 gccaacgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt
    12541 gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt
    12601 gtcacttcgc gtgaaagcca ggtcacggcg tactgttctg gaggcggcgc aaggtaatgt
    12661 aaccgacaac gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa
    12721 aaccaggttt ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg
    12781 gtcagtaagg cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca
    12841 ttcagttttt cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg
    12901 ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag
    12961 tgaccgcaac ttttgtgtcc ctgcggttgt tggcagagta aataacggct cagggcgtag
    13021 attaaagcat catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt
    13081 ccggcctgat agctggctac cagtttttcg aagtcaggtc gtaaccatgg ataccatctc
    13141 atgcgtccaa ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct
    13201 ccagcggctg ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat
    13261 agcgggcgcg ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc
    13321 cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt
    13381 cggggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac
    13441 cacgtcctcc gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg
    13501 tgccgttagc cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa
    13561 acatcagaac ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca
    13621 ccaggcttct taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt
    13681 cgcggatacc cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg
    13741 cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc
    13801 agataatcct gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct
    13861 ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc
    13921 gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta
    13981 tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc
    14041 ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat
    14101 accgaggcaa cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc
    14161 atcgtcacca gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg
    14221 ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta
    14281 ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc
    14341 agttcaagtg cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac
    14401 ggagcctcac gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc
    14461 gggaactgtg cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc
    14521 ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcaac
    14581 cattgaaaaa cccgtgggcg attgatgatc ttatcggcat aaagctgttc accaagcgcc
    14641 agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg
    14701 tcggcaagat ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc
    14761 aataacaaga taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg
    14821 ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca
    14881 tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag
    14941 aggcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc
    15001 gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc
    15061 gccagaacaa attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg
    15121 cctcatcagc gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa
    15181 gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg
    15241 tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata aaccgtaacg gagagaatcc
    15301 gctttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca
    15361 cgactttcag cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct
    15421 gaggccagaa gtcacagaaa atcatcaacc gctgacaagc atcctgtagt cgctggatat
    15481 gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac
    15541 caaactgcgt tgcccgatcg cttactgcca gcgattcctg cttatgaccg ttaattaaga
    15601 acataccggc tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt
    15661 cggaataaaa gctaagaaaa aaggcccgca agcggacctt ttatatgggt gggaaatgac
    15721 aacttagatc tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca
    15781 cagcgtgtat tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca
    15841 accttcatcc gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag
    15901 gatggagtag atagactcta ctgcccccgc cgcacctaac aggtgaccgg tcatagattt
    15961 cgtggagctt accaacacac ggcttgcagc ttcaccgaag atggctttca ccgcctgcgc
    16021 ttcagcttta tcgccagccg gcgtagaagt accgtgcgca ttaacgtagc caatctgact
    16081 cgcttcaatg cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt
    16141 ttctggcggt gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc
    16201 gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct tctagtacca gcataccggc
    16261 accatcgccc agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg
    16321 gttatcattg cgggtagata atgcacgtgc cgcgccaaaa ccaccaacac ccagcggcgt
    16381 actggctttc tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg
    16441 cgcagcatgg ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg
    16501 gccacgcagg ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg
    16561 aacgaagaat gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc
    16621 gatcagtccg aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc
    16681 ttcagttatt tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg
    16741 aatgaaggca tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc
    16801 ctttactaag ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct
    16861 gatgccactc tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg
    16921 agacaacatg cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg
    16981 agggaaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct
    17041 ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt
    17101 gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac
    17161 cagctcaacg gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt
    17221 aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat
    17281 agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta
    17341 taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt
    17401 ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact cataaccacg
    17461 cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc
    17521 gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac
    17581 cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc
    17641 cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac
    17701 aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat
    17761 caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac
    17821 agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt
    17881 tgacagacgg aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt
    17941 cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc
    18001 accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt
    18061 caacatcaga cctttgccgt tagcacctaa ataatcactg atcgactgag caccattttc
    18121 actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg
    18181 gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt
    18241 cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt
    18301 cagggtgtcg acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata
    18361 gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt
    18421 atacaactga cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc
    18481 aacgttattc acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc
    18541 cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg
    18601 cagcgcgcgt tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcctcttt
    18661 atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc
    18721 ttctgcagct tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc
    18781 catagcgccc gtgccttctg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag
    18841 acgcaccgca tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag
    18901 gctgtgaccg gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag
    18961 cgcaacagat gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc
    19021 tggcccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa
    19081 cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga
    19141 accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg
    19201 aaccagcgcg gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc
    19261 cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga
    19321 ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atcgacatac cgagtttttt
    19381 cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga
    19441 acggtcaaga ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc
    19501 aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg
    19561 gtcggcgttt ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga
    19621 gatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat
    19681 aataatagtc ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac
    19741 cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt
    19801 gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct
    19861 ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat
    19921 caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa
    19981 gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg
    20041 agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac
    20101 ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact
    20161 tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgttc tgctaccgca
    20221 gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc
    20281 gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga
    20341 ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta
    20401 acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct
    20461 tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag
    20521 atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc
    20581 ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa
    20641 gatagccgat atagttgata gaagggattg ttttaagcac cgctgaggca tcccgaatac
    20701 tgtcgagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa
    20761 tttccaccac ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc
    20821 tgtcacaatc gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg
    20881 gtaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg
    20941 ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt
    21001 tcaccagctc cagggccata cgcattgaac tcccacgact ggcgcggata gcgtgcgaag
    21061 gccgggcatc actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt
    21121 caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag
    21181 tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa
    21241 aatcccctcc catgacatct aacgccaggg ttagacatgt caaggtatcg tcgccgcccg
    21301 gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg
    21361 tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg
    21421 caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc
    21481 atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga
    21541 ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt
    21601 taagctggct aatacggcaa atgggtttgg cttttgcact tcttcaggca gttcaccaaa
    21661 gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctactggcaa
    21721 ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac
    21781 ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact
    21841 aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag
    21901 cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa
    21961 cgacatggag cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac
    22021 ctgatcaggg gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc
    22081 gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat
    22141 agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg
    22201 gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca
    22261 tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt
    22321 attttagcct ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt
    22381 gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg
    22441 gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta
    22501 attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg
    22561 gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact
    22621 ggactggcgt tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac
    22681 gtccatttcc gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc
    22741 ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta
    22801 gagggacgtg accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta
    22861 cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg
    22921 catccggcaa tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc
    22981 cattatcagg cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt
    23041 caaaccttca tccatcggcg cttcgatacg catcacttca ccggtccccg gatgggtaaa
    23101 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    23161 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg
    23221 tgtatgcaca cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc
    23281 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac
    23341 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt
    23401 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc
    23461 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    23521 tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    23581 ccgcaaacct tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    23641 aggtttattc agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc
    23701 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    23761 gatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    23821 ccgcacttca cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    23881 ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt
    23941 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    24001 actcattttt ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt
    24061 tattgatggt tattaaacca atcaccaaca agaagtgaaa aaactgtgag taagcgggtg
    24121 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    24181 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    24241 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    24301 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    24361 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    24421 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    24481 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    24541 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    24601 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    24661 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    24721 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    24781 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    24841 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    24901 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    24961 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcgagcctt gaactgccgg
    25021 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    25081 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    25141 cggctccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    25201 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    25261 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    25321 agatcccgct gttcagccac taccagatcg agtcgcagat cgaatccgcc ttccagcgtg
    25381 aagtccgtct gccgtccggt ggttccattg ttatcgacag caccgaagcg ttaaccgcca
    25441 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca
    25501 ccaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    25561 tgattgttat cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc
    25621 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    25681 ttggcctgct ggaaatgtcc cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc
    25741 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    25801 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    25861 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    25921 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    25981 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    26041 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    26101 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    26161 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    26221 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    26281 tcagcggtag tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    26341 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    26401 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    26461 atcgtcgcca ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg
    26521 taacggaaaa agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    26581 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    26641 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    26701 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg
    26761 caccggtggt tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac
    26821 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    26881 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    26941 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    27001 cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    27061 tctggatccg ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac
    27121 aggaagaagt acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    27181 ctgttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    27241 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    27301 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag
    27361 aacacgcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg
    27421 aaactgctga ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg
    27481 tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt tgaaccagag atcaccgttg
    27541 agcataacca cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg
    27601 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    27661 gtggtcatac ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    27721 aataattagc tcaaagtaat caagccctgg taactgccag ggctttttta tttcatcttt
    27781 gaatcaacat attaaaattc atcaaatggc atccttgcta accaacaatg caaaataggc
    27841 aaaaaaaatg cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg
    27901 atatgtttta aagcgagctt atttgttgag ctggaagagc gacaatccct gcatatcggt
    27961 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    28021 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    28081 acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacggt
    28141 aaggacatta ttcagcgagt ttttcagtcc acggttggtt ttatccaatg ccgcagcggc
    28201 gatttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc
    28261 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact
    28321 ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc
    28381 gacctgttgc ctaatacttt ctgctccacc gacgtatttc cctttctctt cgctaaacgg
    28441 cgcagtctct gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc
    28501 cagattcagc aactggtcac gaagcccctg gatatccgta gccagcgagg cccggtcatc
    28561 gtcactcaaa gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    28621 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc
    28681 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgctgc
    28741 aatgggatcg tcagaagggt taacgactcg cttacctgtc gacatctgct cgccgtactt
    28801 catccattcc gcctgagaat tggtgatgcc acgcatgttt tgctggtaca tcatctgggt
    28861 actgaaacgc atcttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    28921 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    28981 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    29041 acattacctt gcgtggcgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    29101 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt
    29161 tgcagatcca gcagggcctg accgttgcgg ttatcgctat cacccgcatc ttcttcgctc
    29221 gccatcgcta ttttcgcttc atcggtgatt aatacatcca tgttgacgat agcgtcactt
    29281 actggtttca gcgtgaagct gtcgttaaca gcaggcgttc ctgtaaacgt caactccaga
    29341 ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attactggca
    29401 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatcagt cgccagtacc
    29461 gcggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctgtgttttt cgtgttttgc
    29521 agaaccgcgg gcttaccgat agcaaagaaa tcttcaccag catcgccgtt ggcatcaaat
    29581 ccggctttgt gttggctgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    29641 ttacgcgtct ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    29701 ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc
    29761 gtacgagaag ggtcggcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga
    29821 accagtgagt aaccattggc catcgtgatg ttataagtac cgccatcctg aacgctgact
    29881 tctacgccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    29941 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    30001 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    30061 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    30121 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    30181 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    30241 tcgattttcg acatctgctc atagcgggca gtcaggccgc tactttgcgt ctgcgccgca
    30301 cgtaactggt tggtaataaa agcatcatac tcacgctgca caccagaaac gtagacacca
    30361 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    30421 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    30481 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    30541 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    30601 gattatcaat gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    30661 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatacccc gcgtcctgta
    30721 gggcctgcgc cccctgctcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac
    30781 gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact
    30841 tcgcttttac tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtaactg
    30901 gccctttcca gttgccagag gctttgacac caaacaggtt atagctcggc tcgccgtttt
    30961 cgcggcggat ttgccgttgc ccccaaccag attccagtgc cgcctgagcg aggatcaaat
    31021 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    31081 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    31141 gctgcgaaag cgcctgattt tgataacgca ccacggtttc gagcgggaat ttcatcggtg
    31201 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    31261 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac
    31321 tggtatacag acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    31381 tgcttttcaa catcatctgc acgaacatcc cttccacctg gcgggccacc ggacggatat
    31441 ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    31501 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    31561 acatcccgca ctttgcattg attgcagtat ggacatcaga tccatcggcg tagcgcccag
    31621 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    31681 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    31741 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    31801 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    31861 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    31921 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    31981 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    32041 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    32101 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    32161 cacctggaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    32221 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    32281 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    32341 tccaaacgga ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    32401 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    32461 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    32521 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    32581 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    32641 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    32701 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    32761 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    32821 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    32881 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    32941 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    33001 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    33061 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    33121 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    33181 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    33241 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    33301 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    33361 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat
    33421 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    33481 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    33541 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca ccagaggatt gcgtttcggt
    33601 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    33661 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    33721 accaatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    33781 accagccgtc accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc
    33841 tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt
    33901 gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg
    33961 tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc
    34021 cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc
    34081 gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc
    34141 aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattgctg
    34201 acaagcagag cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa
    34261 aggggcgagg gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc
    34321 atcgacagca gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc
    34381 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    34441 ttactgcctt ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtatcggc
    34501 ccacgcgtgg cctggctttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgttgcact
    34561 tcgctgcccg tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga
    34621 tcgcccggat tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc
    34681 ggcacagcaa ttggccccgc ctcgcctatc accggatgcc cctgaattgt cagttgcccg
    34741 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggtctgc
    34801 acggccagcc agccatcctg ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc
    34861 ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtaggcagt
    34921 gaaagccctt ccaccggcac tgcacgcaag gcgtttaact gcgcgcgaaa accgggcgtt
    34981 gaggcattgg ccagattact ggcggttacc gcctgttgat tcagtgtctg gctggctgct
    35041 cccatcgcag tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    35101 aaccagcgtg ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt
    35161 acgctgggca acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccag
    35221 cgcaccgttg gtcagggtgc caaagtttcc cgtcccggct gtccccaaca gcgccacgcc
    35281 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    35341 aaagttcgcc agtacaatct gccccagcag ttgggtttgt tcgttggaat agttgccgac
    35401 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccgttctg
    35461 ggtggttgcc acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag
    35521 gctaaacgta gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt
    35581 tgaggtcaga acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt
    35641 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    35701 cacgctcatg tcatgagcat taccctgact atcgaaaaca gtcaccgaac cttttttgtt
    35761 atagctatcc gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cactggaatt
    35821 caggttgatc tgcatcgacg ccgtggtggt agttttcgct gccatcaggg tattcgggat
    35881 cgaaatattg gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    35941 acccgtcagc tgtaaacctt gcatattcac caggttacga ttttcatcca gcttaaattg
    36001 tccgttacgg ctgtaaaaca ccgaaccgtt gctgtctacc agacggaaaa aaccattctg
    36061 gctgatagca acgtccagac cacgcccggt gttggtggtc gtgccatcgg taaagtcctg
    36121 agtgataccg gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    36181 agaagccgtg cctgttttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac
    36241 atcgaggttg gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    36301 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    36361 agtgccgaga tccagcgtat taccgttgtt gccgcggatc acaccctgca ccagagcaaa
    36421 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacattgta
    36481 agaaccgttc ggcgcagtgc tgccgtcagt caacgtaccg tcccaggtga aactgtgaac
    36541 tccggcggtc agttcaccaa tatcaatggt gcgcacaacc gcaccatttt tatcggtgat
    36601 ggtggccgta actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    36661 cacagctcct tcttcactgc cggttcccgc aagaacagtg gtgccgggga tcatcacgcc
    36721 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    36781 tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga
    36841 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccag
    36901 caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactgct
    36961 ggtggtactg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt
    37021 ctcctttatt gaccgagcgt aagggtttta agcatcatac ttttcaccgt gttgagcact
    37081 tcaacattgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca
    37141 tcaacgttcg gcatttttac gtagcccttt gcatccgcca gcggattacc cggttcataa
    37201 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    37261 gcacctggtg cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatcg
    37321 ggaccggtca cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac
    37381 tgggcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    37441 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    37501 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta
    37561 ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    37621 gcctgcgccg gaatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc
    37681 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    37741 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    37801 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    37861 caggtatcaa aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    37921 aacgcaaaat gggtcgctat ttatgccgtt gatgaccatt gcggacaggt acaattcacg
    37981 ttgtagaaat ggctgggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcattgc
    38041 gatactgttc agtccgttaa gtgcggcgag caatctcaca tcgcaattgc aaaccttttt
    38101 tagcgcccag ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa
    38161 tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg
    38221 caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtaca
    38281 ggccacagga aattatgtgg ttgccgcgat gcccattgcg cggggaggaa agctggaagc
    38341 tggcaatgtc aaactgaaac gcggacggct ggataccctg ccaccgcgta cggtgctgga
    38401 tatcaatcaa cttgttgatg ccattagcct gcgcgatcta tcacccgatc aacctatcca
    38461 gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca atgtgatcgc
    38521 cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc
    38581 acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg ttgatgcaga
    38641 tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata
    38701 agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc
    38761 gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa
    38821 cagccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca
    38881 agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa
    38941 actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat
    39001 caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt gcagagatcc
    39061 tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac
    39121 atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct
    39181 cactgctggc gacgctggat tacctcgaac agttacgcaa gaaagaaccc aatacagcaa
    39241 atagcgttga tattagtcaa cgctggcagg aaattactgt gaaatcgcag caactacgcc
    39301 aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc
    39361 tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt cagacctcaa
    39421 caacccatcg cggcggtaaa aagatttcaa tctgaatatt atctccggcc tgcactgcag
    39481 gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtacc
    39541 gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaag
    39601 cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg
    39661 ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc
    39721 ggagtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc
    39781 gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa cgccaggttc
    39841 atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg
    39901 cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag
    39961 taggcaatta acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt accgtactgg
    40021 aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact cggcagcgcc
    40081 aacaggaaac aaagacgcaa cccccagtcc atcaaacggt tgtattcatc atgattgcca
    40141 ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac gcccagcaca
    40201 ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga accggaagca
    40261 agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg
    40321 atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa gttaatgcgc
    40381 ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag ctgcaggatg
    40441 ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca
    40501 aacagcgcga aaccaatcat gctgatgtta agcagtgttg gagcaaacgc cggaatcgag
    40561 aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata
    40621 tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcggc agtgtcagcg
    40681 aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc gaccgtcaca
    40741 accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct
    40801 tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga aaatgcccct
    40861 tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt
    40921 gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc
    40981 gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta
    41041 atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc
    41101 tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg
    41161 ccagatcttg tcaacgatac gttgcgccag cactgcctgt tcgccggcgg tttgcggaac
    41221 tgtctgattt tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc
    41281 aagagtgctc tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg
    41341 ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc
    41401 acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac cagccgaaaa
    41461 atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag
    41521 agaggctttg ccacccgaca accacagcgc ggtatccacc acatgcagat aatcatccag
    41581 caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat ccattcttaa
    41641 cgaggctgcg gtggcgagtt gcgtttttaa ctctccgtag agtcgtgcga aacgacggtt
    41701 aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc
    41761 gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt
    41821 actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc
    41881 ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc
    41941 tttcgcgcgc gtaggcgacc aggctccttg taacgtccag tcagacgctg ccgccagcac
    42001 cggtaaccac gctttttgcg caatgccacc taatcccact acgccgatac gtaatttttt
    42061 cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt
    42121 cgatttccag ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc gacatatccg
    42181 tcaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt
    42241 tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt gccagttgtt
    42301 ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc
    42361 gcagttcacc tggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttccg
    42421 ctgcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga
    42481 cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga ttatccagct
    42541 gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt ttctgattac
    42601 aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt
    42661 ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc atgagattct
    42721 ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat
    42781 tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc
    42841 agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt
    42901 tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc
    42961 tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc acgaacaaca
    43021 ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag
    43081 aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg ccagcctgat
    43141 ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct
    43201 gcgtaataat ccgcaagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcga
    43261 tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc
    43321 cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt
    43381 gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa
    43441 agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat
    43501 gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtatcgcag
    43561 gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc
    43621 tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc
    43681 atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg tattttcggc
    43741 ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgc
    43801 gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc
    43861 ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa
    43921 ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc
    43981 ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc
    44041 tgcgccacag gggccgtttt gtttgtgctg tgtgcggcgt tcaatgcgtg gttgttaccg
    44101 gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgt
    44161 gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa
    44221 gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcaccctc tgccgttaaa
    44281 tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg
    44341 agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat aatgtcatta
    44401 agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg
    44461 ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg
    44521 gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc
    44581 gctattggtt atatcggtgg tggctggctg tttgacctgg gcaaatcggc gcaccagcca
    44641 gagcttccgt ggatgatgct gggaattatt ggcatcttca ctttccttgc gctgggttgg
    44701 cagtttagtc agaaacgcgc cgcgcgtcgt ttgctggaac gcgacgcctg atttgatact
    44761 cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag
    44821 ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa
    44881 aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg
    44941 gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc
    45001 atggatatcg ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt
    45061 tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg
    45121 ccgcgttttg ccaaatcagc atttgatgag ttttctactc ccgccgcgcg caaatatttc
    45181 gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt
    45241 ctgattaaga atatcagcga tgatttgcgt gcgctggaca aactgatcgt caaaccgaac
    45301 gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctact gcgtaatctg
    45361 acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    45421 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca
    45481 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt
    45541 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    45601 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    45661 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    45721 ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga caaacttgac
    45781 cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga
    45841 catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    45901 cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg tcgatgcgac
    45961 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt tgaaccaggc
    46021 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagtcaggg
    46081 cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    46141 tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    46201 acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt
    46261 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    46321 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    46381 tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg tggcgtatcg
    46441 ccaacgtatt cttgatgccg tacctgccgg gcacaatttc accccattga tgacctgtta
    46501 tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac
    46561 cgctgcaaaa ctttatccgg caaacgcaac cactaactcc agccacggcg tgacgtcaat
    46621 tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca
    46681 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag
    46741 cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg agcacatcac
    46801 caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac
    46861 tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    46921 gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactggtcgc
    46981 cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa
    47041 agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc
    47101 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    47161 cccgcagttc tatggcttgc cggtcaacga cacattcatc gaactggtac gtgaagagca
    47221 acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc tcgccgggga
    47281 aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    47341 ttacctgtat aaataaccag catattcaac agggggctat tatgcgaatt gaagtcacca
    47401 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    47461 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag
    47521 cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt agcgaaattc
    47581 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    47641 ttgtttttgc cgggtcgccc cggctttttt attttactta caaattttga gtacaaaatt
    47701 tctacactcc ctcaaaataa ttg
//
LOCUS       DAZFAH010000014        46329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFAH010000014 DAZFAH010000000
VERSION     DAZFAH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.014"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHJWB_000751"
     CDS             <1..330
                     /locus_tag="ACHJWB_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4312431.1"
                     /translation="IVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFK
                     PDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVD
                     PSSTAHE"
     gene            complement(314..952)
                     /gene="ytfB"
                     /locus_tag="ACHJWB_000752"
     CDS             complement(314..952)
                     /gene="ytfB"
                     /locus_tag="ACHJWB_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4312432.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1171..1791
                     /gene="fklB"
                     /locus_tag="ACHJWB_000753"
     CDS             1171..1791
                     /gene="fklB"
                     /locus_tag="ACHJWB_000753"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4312433.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2100..3512
                     /gene="cycA"
                     /locus_tag="ACHJWB_000754"
                     /gene_synonym="dagA"
     CDS             2100..3512
                     /gene="cycA"
                     /locus_tag="ACHJWB_000754"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4312434.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3557..4219)
                     /gene="ytfE"
                     /locus_tag="ACHJWB_000755"
     CDS             complement(3557..4219)
                     /gene="ytfE"
                     /locus_tag="ACHJWB_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4312435.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4327..5292)
                     /gene="ytfF"
                     /locus_tag="ACHJWB_000756"
     CDS             complement(4327..5292)
                     /gene="ytfF"
                     /locus_tag="ACHJWB_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4312436.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(5401..6261)
                     /gene="qorB"
                     /locus_tag="ACHJWB_000757"
     CDS             complement(5401..6261)
                     /gene="qorB"
                     /locus_tag="ACHJWB_000757"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4312437.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHTDTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            6350..6730
                     /gene="ytfH"
                     /locus_tag="ACHJWB_000758"
     CDS             6350..6730
                     /gene="ytfH"
                     /locus_tag="ACHJWB_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4312438.1"
                     /translation="MSQVSLSQQMKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6848..8791)
                     /gene="cpdB"
                     /locus_tag="ACHJWB_000759"
     CDS             complement(6848..8791)
                     /gene="cpdB"
                     /locus_tag="ACHJWB_000759"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4312439.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8981..9721
                     /gene="cysQ"
                     /locus_tag="ACHJWB_000760"
     CDS             8981..9721
                     /gene="cysQ"
                     /locus_tag="ACHJWB_000760"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4312440.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9711..10268)
                     /gene="ytfJ"
                     /locus_tag="ACHJWB_000761"
     CDS             complement(9711..10268)
                     /gene="ytfJ"
                     /locus_tag="ACHJWB_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4312441.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10593..10799
                     /gene="ytfK"
                     /locus_tag="ACHJWB_000762"
     CDS             10593..10799
                     /gene="ytfK"
                     /locus_tag="ACHJWB_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4312442.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10861..12204)
                     /gene="paeA"
                     /locus_tag="ACHJWB_000763"
     CDS             complement(10861..12204)
                     /gene="paeA"
                     /locus_tag="ACHJWB_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4312443.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12527..13165)
                     /gene="msrA"
                     /locus_tag="ACHJWB_000764"
     CDS             complement(12527..13165)
                     /gene="msrA"
                     /locus_tag="ACHJWB_000764"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4312444.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13371..15104
                     /gene="tamA"
                     /locus_tag="ACHJWB_000765"
     CDS             13371..15104
                     /gene="tamA"
                     /locus_tag="ACHJWB_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4312445.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15101..18880
                     /gene="tamB"
                     /locus_tag="ACHJWB_000766"
     CDS             15101..18880
                     /gene="tamB"
                     /locus_tag="ACHJWB_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4312446.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPTLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPQGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLEVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            18883..19224
                     /gene="ytfP"
                     /locus_tag="ACHJWB_000767"
     CDS             18883..19224
                     /gene="ytfP"
                     /locus_tag="ACHJWB_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4312447.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            19436..19687
                     /gene="chpS"
                     /locus_tag="ACHJWB_000768"
     CDS             19436..19687
                     /gene="chpS"
                     /locus_tag="ACHJWB_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO4312448.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            19681..20031
                     /gene="chpB"
                     /locus_tag="ACHJWB_000769"
     CDS             19681..20031
                     /gene="chpB"
                     /locus_tag="ACHJWB_000769"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO4312449.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(20111..20641)
                     /gene="ppa"
                     /locus_tag="ACHJWB_000770"
     CDS             complement(20111..20641)
                     /gene="ppa"
                     /locus_tag="ACHJWB_000770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4312450.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            20951..21907
                     /gene="ytfQ"
                     /locus_tag="ACHJWB_000771"
     CDS             20951..21907
                     /gene="ytfQ"
                     /locus_tag="ACHJWB_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4312451.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22047..23549
                     /gene="ytfR"
                     /locus_tag="ACHJWB_000772"
     CDS             22047..23549
                     /gene="ytfR"
                     /locus_tag="ACHJWB_000772"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4312452.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            23563..24585
                     /gene="ytfT"
                     /locus_tag="ACHJWB_000773"
     CDS             23563..24585
                     /gene="ytfT"
                     /locus_tag="ACHJWB_000773"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4312453.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            24572..25567
                     /gene="yjfF"
                     /locus_tag="ACHJWB_000774"
     CDS             24572..25567
                     /gene="yjfF"
                     /locus_tag="ACHJWB_000774"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4312454.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(25600..26598)
                     /gene="fbp"
                     /locus_tag="ACHJWB_000775"
     CDS             complement(25600..26598)
                     /gene="fbp"
                     /locus_tag="ACHJWB_000775"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4312455.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            26774..28147
                     /gene="mpl"
                     /locus_tag="ACHJWB_000776"
     CDS             26774..28147
                     /gene="mpl"
                     /locus_tag="ACHJWB_000776"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4312456.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAVARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(28230..28781)
                     /gene="yjgA"
                     /locus_tag="ACHJWB_000777"
     CDS             complement(28230..28781)
                     /gene="yjgA"
                     /locus_tag="ACHJWB_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4312457.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDALAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            28875..30227
                     /gene="pmbA"
                     /locus_tag="ACHJWB_000778"
     CDS             28875..30227
                     /gene="pmbA"
                     /locus_tag="ACHJWB_000778"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4312458.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            30410..30796
                     /gene="cybC"
                     /locus_tag="ACHJWB_000779"
     CDS             30410..30796
                     /gene="cybC"
                     /locus_tag="ACHJWB_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4312459.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMEILNDNLKVVEKADN
                     ASQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(30841..31305)
                     /gene="nrdG"
                     /locus_tag="ACHJWB_000780"
     CDS             complement(30841..31305)
                     /gene="nrdG"
                     /locus_tag="ACHJWB_000780"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4312460.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(31463..33601)
                     /gene="nrdD"
                     /locus_tag="ACHJWB_000781"
     CDS             complement(31463..33601)
                     /gene="nrdD"
                     /locus_tag="ACHJWB_000781"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4312461.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(33995..35650)
                     /gene="treC"
                     /locus_tag="ACHJWB_000782"
     CDS             complement(33995..35650)
                     /gene="treC"
                     /locus_tag="ACHJWB_000782"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4312462.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(35700..37121)
                     /gene="treB"
                     /locus_tag="ACHJWB_000783"
     CDS             complement(35700..37121)
                     /gene="treB"
                     /locus_tag="ACHJWB_000783"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4312463.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(37240..38187)
                     /gene="treR"
                     /locus_tag="ACHJWB_000784"
     CDS             complement(37240..38187)
                     /gene="treR"
                     /locus_tag="ACHJWB_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO4312464.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHLQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            38372..38425
                     /gene="mgtL"
                     /locus_tag="ACHJWB_000785"
     CDS             38372..38425
                     /gene="mgtL"
                     /locus_tag="ACHJWB_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4312465.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            38566..41262
                     /gene="mgtA"
                     /locus_tag="ACHJWB_000786"
     CDS             38566..41262
                     /gene="mgtA"
                     /locus_tag="ACHJWB_000786"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4312466.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGRLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(41468..41854)
                     /gene="ridA"
                     /locus_tag="ACHJWB_000787"
     CDS             complement(41468..41854)
                     /gene="ridA"
                     /locus_tag="ACHJWB_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4312467.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGSMIITSGQIPVNPKTGEVPT
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(41927..42388)
                     /gene="pyrI"
                     /locus_tag="ACHJWB_000788"
     CDS             complement(41927..42388)
                     /gene="pyrI"
                     /locus_tag="ACHJWB_000788"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4312468.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(42401..43336)
                     /gene="pyrB"
                     /locus_tag="ACHJWB_000789"
     CDS             complement(42401..43336)
                     /gene="pyrB"
                     /locus_tag="ACHJWB_000789"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4312469.1"
                     /translation="MANPLYQKHIISINNLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLIL"
     gene            complement(43340..43474)
                     /gene="pyrL"
                     /locus_tag="ACHJWB_000790"
     CDS             complement(43340..43474)
                     /gene="pyrL"
                     /locus_tag="ACHJWB_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4312470.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(43755..44150)
                     /gene="yjgH"
                     /locus_tag="ACHJWB_000791"
     CDS             complement(43755..44150)
                     /gene="yjgH"
                     /locus_tag="ACHJWB_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4312471.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(44281..44994)
                     /gene="bdcA"
                     /locus_tag="ACHJWB_000792"
     CDS             complement(44281..44994)
                     /gene="bdcA"
                     /locus_tag="ACHJWB_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4312472.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            45065..45658
                     /gene="bdcR"
                     /locus_tag="ACHJWB_000793"
     CDS             45065..45658
                     /gene="bdcR"
                     /locus_tag="ACHJWB_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4312473.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            45803..46255
                     /gene="tabA"
                     /locus_tag="ACHJWB_000794"
     CDS             45803..46255
                     /gene="tabA"
                     /locus_tag="ACHJWB_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4312474.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT        10942 a        11660 c        12442 g        11285 t
ORIGIN      
        1 attgtgcata gtgaaaatca aagtaaaggt gtgcattatg caaaatcaca gcgtctctta
       61 gaaattaacc atgcgcactt gcagttaatg gaatccttat tggatgaagg gaagaaacac
      121 aatattttta agccagatat cgacccattg caggtgaata ttaatattgc tgcgcttggc
      181 ggatattatt tgatcaacca gcatacgctt ggcctggttt atcatatcag tatggtttcc
      241 ccccaggcac tggaagccag acgtaaggtc atcaaagaga caatccttag ctggcttttg
      301 gttgaccctt catctaccgc gcacgaataa aactgccatc cggctggcgg gtgaacagga
      361 cctgttgatt attccccgta tcaatggtta agcccgtcac cacgccgctg gcgttctggc
      421 ggattttcac catttgcccg ttttgcaaat tactcagcgg cttacccgcg ccttctacct
      481 gcgccattgc atagacatcg gtagcgggca gtccgtggtc gcggaacagc tgcgccatcg
      541 ttttaccggg ttcgacacgg taagagcgcc attgattatc aattccgcta tctggctgga
      601 atggttgtgt ttgggtagtt tgcggctgtt cttctggctg gccttcctgt ataggttctg
      661 gagcgaccgg agccacctga tcaggatcat tttgtggtgt gactaattgc gcccgcagct
      721 gttcttctgt cggcggttgt gattgcgact gaatatctaa ctgcgcttca cgcgtcacca
      781 ccggcgcgtt gggcgtatca tcagatggaa gcagaaatcc gaccaccaat acaatggcgg
      841 caataatgat cccccgacga tgcatcggcg gcagcgggtc catgaagcga aaattgtccg
      901 gcgcgtgcca gactttctcc agggttggtt ttagttcaaa gcgcccgggc atggtttcct
      961 cctgctccgc gtcttgttcc tcaatcatag cctatgaata agctaacgct atgatgtccg
     1021 tggtaaaccc gcctttatta tattcatacg ggatattgct attgtttctt tttccccggg
     1081 atttgtcatc attcctgcga caaagtttta cccaaagaag tgtggctgat atgctgcccg
     1141 ctacttttat accctaagaa aggaaatacg atgaccaccc caacttttga caccatcgaa
     1201 gcgcaagcaa gctacggcat tggtttgcag gtagggcaac aactgagtga atctggcttg
     1261 gaagggctgc tgccagaagc actggttgca ggtattgccg atgcgctgga aggcaaacat
     1321 ccggctgttc cggttgatgt ggtgcatcgc gcgctgcgtg aaatccacga gcgcgccgat
     1381 gccgttcgtc gtcagcgttt ccaggcgatg gctgctgaag gtgtgaaata cctggaagaa
     1441 aacgccaaaa aagaaggtgt gaatagcacc gaatctggcc tgcaattccg cgtgatcaac
     1501 cagggtgaag gcgcaattcc ggcacgtacc gaccgcgttc gtgttcatta caccggtaaa
     1561 ctgatcgacg gcaccgtgtt tgacagctcc gttgctcgtg gtgaacccgc tgaatttccg
     1621 gttaatggcg tgatccctgg ctggattgaa gcactgactc tgatgccggt aggttctaaa
     1681 tgggaactga ctatcccgca ggaactggca tatggcgagc gcggcgcagg cgcatccatc
     1741 cctccgttca gcaccctggt gtttgaagtc gaactgctgg aaatcctcta agcagcgcat
     1801 tctgttcccc tcgaaccaga ggggagcagg cattcagcaa taaacccttc agtttgccaa
     1861 acggcgctat tttgtgttgc aaagaccccg taagcgtgta tttttgtgag ctgtttcgcg
     1921 ttatcaccgt gatatgacac tcactttaaa cataaaatta acattagatc taaatcttag
     1981 tattcatccc gcgtattgtt acctaatatc gatgagtccc aatacagatt cgtcgtatca
     2041 tagactgact aaaggccgta gagcctgaac aacacagaca ggtacaggaa gaaaaaaaca
     2101 tggtagatca ggtaaaagtc gttgccgatg atcaggctcc ggctgaacag tcgctacggc
     2161 gcaatctcac aaaccgacat attcagctta ttgccattgg cggtgccatt ggtacggggt
     2221 tgtttatggg gtctggcaaa acgattagcc ttgccgggcc gtcgatcatt ttcgtttata
     2281 tgatcatcgg ttttatgctc tttttcgtga tgcgggcaat gggggaattg ctgctttcga
     2341 atctggaata caaatctttt agtgacttcg cttccgattt actcgggccg tgggcaggat
     2401 atttcaccgg ctggacttac tggttctgct gggttgtaac cggtatggca gacgtggtgg
     2461 cgatcactgc ttatgctcag ttctggttcc ccgatctctc cgactgggtc gcctcgctgg
     2521 cggtgatagt gctgctgctg acgctcaatc tcgccaccgt gaaaatgttc ggtgagatgg
     2581 agttctggtt tgcgatgatc aaaatcgtcg ccattgtgtc gctgattgtc gtcggcctgg
     2641 ttatggtggc gatgcacttt cagtcaccga ctggtgtgga agcgtcattc gcgcatttgt
     2701 ggaatgatgg cggctggttc ccgaaaggtt taagtggctt ctttgccgga ttccagatag
     2761 cggttttcgc tttcgtaggg attgagctgg taggtacaac cgctgcggaa accaaagatc
     2821 cggagaaatc actgccgcgt gcaattaact ctattccgat ccgtatcatt atgttctacg
     2881 tcttcgcgct gattgtaatt atgtccgtga cgccgtggag ttcggtagtc ccggagaaaa
     2941 gcccgtttgt tgaactgttc gtgttggtgg gtttgcctgc ggctgccagc gtgatcaact
     3001 ttgtggtgct gacctctgcg gcgtcttccg ctaacagcgg cgtcttctct accagccgta
     3061 tgctgtttgg cctggcccag gaaggtgttg caccgaaagc gttcgctaaa ctctctaagc
     3121 gcgcagtacc cgcgaaaggg ctgaccttct cctgcatctg tctgctcggc ggcgtggtga
     3181 tgctgtatgt gaatcccagc gtgattggcg cgttcacgat gatcacgacg gtttccgcga
     3241 ttctgtttat gttcgtctgg acgattatcc tttgctcgta cctggtgtat cgcaaacagc
     3301 gtcctcatct gcatgagaag tcgatctaca agatgccgct cggcaagctg atgtgctggg
     3361 tatgtatggc gttctttgtg ttcgtgatcg tgctgctgac actggaagat gacactcgcc
     3421 aggcgctgct ggttaccccg ctgtggttta tcgcgctggg gttgggctgg ctgtttattg
     3481 gtaagaagcg ggctgctgaa ctgcggaaat aaccgcatta tcatgctgga tggcgcaatg
     3541 ccatccagct tttagatcac tcacccgcta gggcgcgtgg gaacagtaca ttgttttcca
     3601 gactgatgtg atccatcagg tcatcaatca gttcattaat gccgttatac atcgctttcc
     3661 aggtggtgca ggcttctggc ggcggtgtga cgttattggt agtgtgtttg atcacttcca
     3721 gcagttcgcc cgcttcatcg tgctcgcttt ccattacgct gattggcccc attgcctggc
     3781 tgcccatgcc ttgtttgatc atcgggaaga ggatctgctc ttctttcatc atgtggctgg
     3841 aaagctcttc atgcagcatg gtcaggtatt ttgtcagccc ttttggcacg ctcggtttgt
     3901 cggcgtgaac gcgctcgact ttagtcgctt gcaggatcag ctccggcagt tgctcgcggt
     3961 gacgatcgtg gtagcgcacg atgatatggt caatgatttc tgccagcggg gcgctacgcc
     4021 agtctttctc aatcggttgt tcagcaagct ttgccagttc agcttcaatg acctcaacat
     4081 ccagttcttt acgtgccgcc gcgcgcgcca gcgtctgctt accgccacag cagtaatcca
     4141 tatcatattt acgaaacaga gccgaagcgc gagggataga gagcgccagt tcacctaaag
     4201 gttggtcgcg ataagccata gctgatacct cattcttaat aagataagat gtattttaaa
     4261 tgcatcttta aggtgaatgc tataaccctt acgtagcaga ggcttattta actcacagcc
     4321 agtatgtcaa ctttctgaga cagattcagt taaaggcttt tccggttttg ctctgactgc
     4381 aatgaccacg ccaaccacta acagcgcgat accgctcagc gtcatcagcg gcggcatttg
     4441 ctggcggagt aaaaaggtgt acagcaaacc tgccagcgtt tcgaaaacaa tcagcggccc
     4501 gagaatcact gtcggtaatc gctggctggc gacgttccag cagagtgcgc caacccatga
     4561 gcaaagcacg gctatcgcaa ccatcagact aataaacacc agcggacggg ggccaaaagg
     4621 taaggagaag tccggcgttt gtatattcag ccagtaacag gcgacgagat agccgatgag
     4681 agaaaccggc agcgtgacca gcgcctgcgc cgtcgcccac atcatcggat gtttgtcggg
     4741 attttcccgc agccagcggg cgttgcgcag ggcataccat gcccagcaga ccacggaaac
     4801 taacgctagc acgatgccag aggtataacg tgcccagtca aaatcgggga gtccgtggtt
     4861 taactcagca atattcacac tcgccaggcc gatgccaata caaatcagtg ccggggcgag
     4921 ttttccccac gcgagtttgc cgtcgcgctg gctataaagc agattggcaa agacaggaat
     4981 gaccaccggc agggtgccga taatcatcgt ggaaacaggc gcgccagtac gttgaatggc
     5041 actggcaagg cagaagtaat aaatgaggtt gcccatcata gtgagcatca aggcggtaag
     5101 ccagtcccga cgcgccaact gacgcagacg cacgcgtccc agccaggcaa tgggcagcgc
     5161 aattaaccct aacgccagat aacgccccat cgactgcaac atcgccgggt attccggcac
     5221 gatcaacggc ccgacaaaaa taagccccca catcaaccct gctaacaggg cgtacagcac
     5281 gccgctaatc attactggca tccattggtc tgtcagaaga aacgttcagg aagcagacat
     5341 gattgctccc ggcatcccgt cattatcaga gaggatgcgg gaggatgcca ctttaattaa
     5401 ctatttatta acattaaaaa gatggcttac gctttcggct aacgttgtcg ttgggcggcc
     5461 aatcaatttg ctcagcgttt tgctgtcatc aaacagaccg cctttcgatg cgccaacgtc
     5521 agaatccgcc agcatatccg ccagtccgtc aggcaggcca acgcttttca gcgccgcagc
     5581 gaaatcggtt tcgctcagat tttgataggt aacctgtttg ccgctctgtt tggtcagctc
     5641 tgccgctaac tgtgtcaacg tccaggcact atcgcccgcc agctcgtaaa ccttgccttc
     5701 gtgaccggct tcgctaatca cgcgtgccgc agctgccgca taatctgccc gcgttgccga
     5761 ggcaattttg ccatcgcccg cggcaccgat aaatacgccg tgctccagtg ctgccggggc
     5821 gctggcgagg tagttttcgc tgtaccagcc gttacgcagc agggtgtaaa cgatgccaga
     5881 atcagccaac attttctccg tctcaatgtg ctcatcggcg aggccgagcg gggaggtatc
     5941 tgtatgtagc aggctggtat aagcgataaa tttcacgcca gccgcctttg cggcattaat
     6001 aacattacga tgctgcgggg cacgttgacc cacttcgctg gaagagatca gcagtagttt
     6061 ttccactccc tgaagtgcag atgtcagtgc ggcttcatcg ccgtagtcag cctgacgcac
     6121 ggtaatgcct tgtgctgcca gagcctgggc ttttgccgga ttacgaacga tagccactat
     6181 ttggctggca ggaaccgttt tcatcaagga ttcaataata tagtgaccaa gttggccagt
     6241 ggcaccagta atagcgatca tgggaagtct ccatcgtttt gtcttgtgtt taaatacgct
     6301 aacacctaaa cttactttta gtaagtacgt acaaaaaggt aagtatgaaa tgagtcaggt
     6361 tagcctgtcg caacaaatga aagagggcaa tctctttgcg gaacagtgcc cgtcgcgcga
     6421 ggtgttgaaa cacgtcacca gccgttgggg ggtgttgatt ctggtggcgc tacgcgaagg
     6481 tactcatcgc tttagcgacc tgcggcgcaa aatcggtggg gtgagtgaaa agatgcttgc
     6541 gcagtcgtta caggcgttag aacaggatgg ttttcttaac cgtatcgcgt atccggtggt
     6601 gccgccgcat gtggaatata gcctcacgcc gctgggcgag caggtgagcg aaaaggttgc
     6661 tgcactggcg gactggattg agttgaattt gcccgaggtg ttggcggtgc gggatgaacg
     6721 agcggcataa ctgccggatg cgcggcctac ggtgtatgac ttgtagggcg gataagatgc
     6781 gccagcatcg catccgccaa taaatgccgg atgcgattga tgatttatag gccgaaaaag
     6841 aagtgtttta cttactcaaa tccacctgat aaatcgcaaa cccgatatca tcggtcgcga
     6901 ctttattcat cggatactgc cctttctctt taataaacgc tgcggcttta tctgacgggg
     6961 aagtttcgaa acggatatcc agtttcttat cggcagctat cggtgctaaa cgccagttgt
     7021 tatctgccgc cggatgaatt tcccccgcac gtttcgactc atcagcaatc cacgctgcca
     7081 gcaccgagcg gttctcatcc ggtgaagcaa aagcgatatg gctgtcgccc gtaccggcaa
     7141 atttgccgcc gtaagcgcga tagttattgg tggcaacgag gaacatggcg ttcggatcaa
     7201 tcggcttgcc gttaaaggtc aggttcttaa tcctttccgc attggcatta atcatctggc
     7261 actcgccgtc ataacgggcg ggttgggtaa catcaatctg ataattcaca ccatcaatca
     7321 catcaaagtt ataagtgcgg aaaccatccc agttaatgag tgactgtggt ttcgtgctgt
     7381 taggatcaat ctggttaaac tgtccggcag agcattccag ccactctttc acctctttac
     7441 cgctggcttt caccacaatc agcgtattgg ggtagagata aagatcggcg gcattacgga
     7501 aggtcaactg gcctttttcc acctccacat agcttgccgg gtcatttttg cgaccaccaa
     7561 ctttaaacgg tgcggcagct gaaagcactg gcagttttgc cagatccgga tcgccctgaa
     7621 tgtaatgctc gacatacgct ttttgcgcgt tgttcaccac ctgcacggtc ggatcgtcct
     7681 gcactagtgc cagatagcta tacatattgt cggcagattt accgattggc ttgctgacga
     7741 actggcgtgt ggcatcgtga tcggctttga gtgtttctac cagcttgctg tcttccgccg
     7801 cgagggattt tttattagcg atgtcgtaaa tcggtcgtgc ttccgctttc gcctgcgtca
     7861 cctgccattt accgctgtca ttactgagtt gtaagtcgac cacaccaaga tgatcgcccc
     7921 acatgcctgg cattaccgcc ggaacaccat tcagcgtgcc tttcgcgata tcagctcctt
     7981 cgatatcagc aaaatcttta cttgggaaaa cggcgtgagc atggccaaac ataatggcgt
     8041 taacgcccgg aatttcactg aggtaataaa ctgagttttc cgccatcact ttatacggat
     8101 cggcagacag cccggaatgc gccagaacga caacgacatc ggcacctttc tcgcgcattt
     8161 caggcacgta tttgcgcacg gtttcggtaa tatcattcac cgtcactttc ccggataaat
     8221 tagctttatc ccagcccatg atttgcggcg gtacgacgcc aatatagcca atcttcagcg
     8281 tctgtttttt tccgtcttta tcgaccactt cggtgtcttt aattaaatac ggtgtaaaca
     8341 ttggctgttt ggttctggcg tcaatgacgt tggcatttac ataagggaat ttcgctcccg
     8401 ccagcgcatt tttcaggtaa tccagaccgt agttaaactc atggttgcca agcgtgccga
     8461 cggtatagtc cagcgtattt aatgccttat acactgggtg aatatcacct gcttttaatc
     8521 ctttcgccga catgtaatct gccagcggac tcccctgaat caaatcgccg ttatcgacca
     8581 gtacgctgtt tttcacttca ttgcgggcat cgttaatcag gcttgccgta cgtaccagtc
     8641 cgaatttttc cgtggcggtg tctttgtaat aatcgaaatc catcatgttg ctatgcagat
     8701 cagtggtttc catgatacgc agatcgaccg tcgctgcatt cacactggcg gcaatcagcg
     8761 tggccaggag cgttgcgcta aacttaatca tcagggacat ccttttatca tcgggaatac
     8821 gaaagaaaag agagaataaa cgtcttactt atagaacagt gaagaatgcc acaattttac
     8881 gctttgaaaa tgatgacact atcacagttg gcgcattcat taacgatagg gtataagtaa
     8941 aacaataagt taacaccgct cacagagacg aggtggagaa atgttagatc aagtatgcca
     9001 gcttgcacgg aatgcaggcg atgccattat gcaggtctac gacgggacga aaccgatgga
     9061 cgtcgtcagc aaagcggaca attctccggt aacggcagcg gatattgccg ctcacaccgt
     9121 tatcatggac ggtttacgta cgctgacacc ggatattccg gtcctttctg aagaagatcc
     9181 tcccggttgg gaagtccgtc agcactggca gcgttactgg ctggtagacc cgctggatgg
     9241 tactaaagag tttattaaac gtaatggcga atttaccgtt aacattgcgc tcatcgacca
     9301 cggcaaaccg attttaggtg tggtgtatgc gccggtaatg aacgtaatgt acagcgcggc
     9361 agaaggcaaa gcgtggaaag aagagtgcgg tgtgcgcaag cagattcagg tccgcgatgc
     9421 gcgcccgccg ctggtggtga tcagccgttc ccatgcagat gcggagctga aagagtatct
     9481 gcaacagctt ggcgaacatc agaccacgtc catcggctct tcgctgaaat tctgcctggt
     9541 ggcggaagga caggcgcagc tgtacccgcg tttcgggcca acgaatattt gggataccgc
     9601 cgctggtcat gctgtggctg cggctgccgg agcgcacgtt cacgactggc agggtaaacc
     9661 gctggattac actccgcgcg agtcgttcct gaatccgggg ttcagagtgt ctatttatta
     9721 attaatttat gcagcaggtc catcacttgc tgcacctctt cctgagtaag cgccccgtct
     9781 ttggcccatt gcacgcgacc gtctttatcc agcaccacta cagcggaact tttttcatcc
     9841 agctgccagg caccgcgtgc gacgccattg ctatcaacaa taaactgcga ccagggataa
     9901 agctttttat tactctccag actgctgcgc acaaacatgc cggaacccgg aattgcgtcg
     9961 tcggtgttaa caatggtggt ggtctggtaa cgatcgtgcg gtaacttcgc tgatttaatc
    10021 gcttcaatca gcgtcgcgtt tttctctttt gcagaggtgc gaccagcaat atgttgcagt
    10081 actcgcactt ttcccactaa ctgcgcgctg ttccaggttt tgtagctaaa ctgatcttta
    10141 tcaagcacca actcgccacg atcggtgatg ccaatcggcg gcactcgctg accggtttcg
    10201 aactgatgtg cggaagccat catcggcaac agcaggcagg tgagtgccag aatcttgcgt
    10261 agggtcatgg tgtttccttc ttatgatatg caggtgatcc gaccacttgg gtctgagttt
    10321 taatcatatg tgctatttat cgaatttccc gcaagtgtga tgccagtttg cggtcaagcg
    10381 cacaaatcat atgaaaaatg aatgcttata ctgaagaccg cgcttcggta aaaagataat
    10441 tctgaataat tgtaaccttt aggtaaaaaa agttatacgc ggtggaaaca ttgcccggat
    10501 agtctatagt cactaggcat taaaatttgc gcctcataat agttgggccg attgtggcac
    10561 cgcacaggcg taatactcag caggagataa caatgaaaat tttccaacgg tacaacccac
    10621 ttcaggtggc gaagtacgta aagatcctgt tccgtggacg gttatacatc aaggacgttg
    10681 gcgcttttga atttgataag ggtaagattc ttatcccaaa agtgaaggat aagctgcatt
    10741 tgtcagtgat gtccgaagtt aaccgtcagg ttatgcgtct gcaaacagag atggcttaac
    10801 caaagtgcta tgcagtaata agacggctcc tgattcagga gccgttgatg tttctggggg
    10861 ttacgcgacg ctttcttctt tatctttagc gtcaggcagt tttggcgaaa gggcggtggc
    10921 cttgctgtcg atccgggtca ccagcagttg gtcgatacgg tagttatcga tatccaccac
    10981 ttcaaatttg tagccggcga atttcaccga atcggtgcgt ttcgggatct tacgcagcat
    11041 aaacatcata aagccgccga tggtttcgta gttgcccgac tgcgggaact cgtcaatatc
    11101 cagcacgcgc atgacgtcgt caatcggggt gccgccgtca atcagccatg agttctcatc
    11161 acgggcgaca atctgctctt ccagcccctg accgaccaga tcgcccatca gcgtggtcat
    11221 cacgtcgttg agggtgatga tccccactac cagcgcgtac tcgttcatga tcaccgcgaa
    11281 gtcttcacct gcggttttaa aactttccaa cgcttctgaa agggttaacg tatccggcac
    11341 aatcagcgtg ttgcgaattt gtacgccgct gttcagtgcc aggctttggt tagccagcac
    11401 gcggttcagc aggtctttag aatcgacata tccgatgatg tggtcaatat cttcattaca
    11461 gacgagaaac ttagagtgcg gatgttccgc caccttattc ttcagacttt gctcatcttc
    11521 gtggagatca aaccaaatca cgttttcacg cggtgtcatt gaagacggaa cggtacggga
    11581 ttccagctca aagacgtttt caatcagctc gtgttcctgt ttacgtaaca cgcctgccag
    11641 cgcaccggct tccactaccg cgtagatgtc atcagaagtg atgtcatctt tacgtaccat
    11701 tggcagtttg aaaatacgga agattatgtt cgccagtccg ttgaagaacc acaccagcgg
    11761 ggtgcaaacg tacaggcaga agcgcatcgg gttgatgata cgcaaagcca cagcttctgg
    11821 cgcaatcata ccgatgcgtt tcggggttaa atccgcaaac aggataaaca tgccagtcac
    11881 taacgagaaa gagagaataa agctcagttg ctcagagagc tctgccgaca tatagcggga
    11941 gaacaggctg tgaaaagctg gagaaaatgc cgcatcaccg acgataccgc cgagaatcgc
    12001 cactgcgttc agaccgattt ggaccacggt aaagaacatg ccgggatttt cctgcatatt
    12061 cagaacgcgt tgggcattta tattgccttc atcagccagc agtttaagtt tgattttgcg
    12121 tgaggcggca agcgagatct cggacatcga gaagaacgca cttacagcga tcaagcagag
    12181 tatgactaaa atactgttta acatatctta tccgaccttt caggccagat cctcggaagg
    12241 ggaagttgat taatttgtgt gaaatacaca ttgaaagccg attcgaagaa agtgaaccgg
    12301 cattttcagt ggctagtata gcgtaaggta ctgtaaagtc gccagagggt taaaattcag
    12361 actgctgata aatgcaatac tgcctgatgc gctatgccga tcaggcctac gcagtccttg
    12421 caatatattg aatttggacg attttgtagg cctggtaagt cgtttaagcc gcatcaggca
    12481 tagacaaaga gcactttatc aacaaactac atttgtaccc gtaacgctat gcttccggcg
    12541 gcagacagac gccaattccg ccaattccac agtaaccata tgggttttta tgcagatatt
    12601 gctggtggtc atcttcggca taataaaacg gtgtggcgtt agcgatttcc gtggtgatgt
    12661 gacgatcgtc atcggcggca agcatcgccg cctgaaaacg ttccagactg gcgcgagctg
    12721 cggcatcctg ttctggggtc agcggataaa tcgctgaacg atactgcgtg ccgtggtcat
    12781 tgccctgacg cattccctgg gcgggatcgt gattctccca aaatacctgt agcaactgct
    12841 catagctgat gacggaagga tcgtaaacaa tgcgtaccgc ttcggcatga cccgtatcac
    12901 cggagcacac ttcccgataa gtcggatttg gcgtatagcc tccggtatag cctgcggcgg
    12961 tgctgtaaac gccgggtaac tgccagaaca gacgctccac accccagaaa caacccatcg
    13021 caaaaatggc aatctccatt ccgtcaggta cattggtcat tgagtgaccg ttgaccgcat
    13081 gcagcgtggc tacgggcatc ggggtgttac gtccaggcag ggcatcggcg ggggaaacca
    13141 gatgcttttt atcaaataaa ctcatggtgt cgctctcccg aaaatcggtc attggggtta
    13201 aggttgtaac aagaggcgta tttgcacaca ataaccaccg tgaataggtc taaagtaaaa
    13261 cataagaaat atttggggtt tagtctgctt tttaatccat attactggat ttttgttaag
    13321 ccgtttaacg gcgttccagg ggcaggaaaa aaggatattc aggagaaaat gtgcgctata
    13381 tccgacagtt atgctgtgta agcttactct gcttaagcgg atctgccgtc gccgcgaacg
    13441 tccgtctaca ggtcgagggg ttatcgggac agctggaaaa gaacgttcgt gcgcagcttt
    13501 ctacgattga aagtgatgaa gtgacgccag accgtcgctt tcgcgcacgc gtcgatgatg
    13561 ccatccgcga aggtctgaaa gcgctgggtt attaccagcc gaccattgaa tttgatctcc
    13621 gtccaccgcc aaagaaaggg cggcaggtat tgatcgccaa agtcacgcca ggtgtgccgg
    13681 tgttaattgg cggcaccgat gtggtattgc gcggcggcgc gcggaccgat aaagactatt
    13741 tgaaattgct cgatactcgc ccggctattg gtacggtact gaaccagggc gattatgaaa
    13801 atttcaaaaa gtccttaacc agtattgcgt tgcgtaaagg ttatttcgat agcgaattta
    13861 ccaaagcgca gctgggcatt gcgctcggcc tgcataaagc cttctgggat attgattata
    13921 acagtggcga acgttaccgc tttgggcatg tgacctttga aggatcacaa atccgcgatg
    13981 aatacctgca aaatctggtg ccgtttaaag agggcgatga gtacgaatcg aaagatctgg
    14041 cggaacttaa ccgtcgactt tctgctaccg gctggtttaa ctcggtcgta gtggctccac
    14101 aatttgataa agcgcgcgaa acgaaagtat tacccttgac gggcgtggtt tcgccgcgaa
    14161 ctgaaaacac catcgaaacc ggggtcggtt actctacgga cgtgggaccg cgcgtgaaag
    14221 cgacgtggaa aaagccgtgg atgaactctt atggtcacag tctgaccacc agtaccagta
    14281 tttccgcgcc ggaacagacc ctcgacttca gctataaaat gccgctgctg aagaatccac
    14341 tggaacaata ttatttggtg cagggcggtt ttaagcgcac tgacctgaac gataccgaat
    14401 ctgactccac tacgctggtg gcttctcgct actgggatct ctccagcggc tggcagcgtg
    14461 ccattaacct gcgctggagc ctcgaccact ttacccaggg tgaaattacc aacaccacga
    14521 tgctgtttta tcctggggtg atgattagcc gcacgcgttc tcgtggtggc ctgatgccaa
    14581 cctggggcga ctcgcaacgc tactctatcg actactccaa cacggcctgg ggctcagatg
    14641 tcgatttctc cgttttccag gcgcagaacg tctggatccg cacactgtac gatcgccatc
    14701 gttttgtgac acgcggcacg ctgggctgga ttgaaacggg tgatttcgac aaagtaccgc
    14761 cggatctgcg tttcttcgcc gggggcgatc gcagtattcg cggctacaaa tacaaatcta
    14821 tcgccccgaa atacgccaac ggtgacctga aaggggcctc gaagctgata accggatcgc
    14881 tggaatacca gtacaacgtg accggaaaat ggtggggcgc ggtgtttgtc gatagtggcg
    14941 aagcggtaag cgatattcgc cgcagcgact ttaaaaccgg taccggggtc ggcgtacgct
    15001 gggaatcgcc ggtcgggcca atcaaactcg attttgccgt accggtcgcg gataaagacg
    15061 agcacgggtt acagttttac atcggtctgg ggccagaatt atgagtttat ggaaaaaaat
    15121 cagcctcggc gtggttatcg ttatcttact gttgctggga tcggtggcgt ttctggtggg
    15181 caccaccagc ggcctgcatc tggtatttaa agcggcggat cgctgggtgc caggactgga
    15241 tattggcaag gtcaccggcg gctggcgcga tctcaccttg tctgacgttc gttatgagca
    15301 gccaggcgtg gcggtaaaag cgggcaatct gcatctggcg gtcgggcttg agtgcctgtg
    15361 gaacagtagc gtttgtatta atgacctggc gctgaaagac attcaggtca acatcgacag
    15421 taaaaaaatg cctccttctg aacaggttga agaagaggaa gatagcggtc cgctggatct
    15481 ctccacgccg tatcccatca ccctgacacg ggtggcgctg gacaacgtca acatcaagat
    15541 tgatgacacc acggtgtcgg tgatggactt cacctccggc ctgaactggc aggagaaaac
    15601 cctgaccctg aaaccgacgt cgctgaaagg cctgctgatt gctctgccga aagtggcgga
    15661 agtggcgcag gaagaagtgg tcgaaccgaa aattgaaaat ccgcagccgg aggaaaagcc
    15721 gctcggcgaa acgctgaaag atctcttttc tcgcccggta ttgccggaaa tgaccgactt
    15781 gcatttgccg cttaacctga acattgaaga gtttaaaggc gagcagttac gcgtgacggg
    15841 cgacacggac atcaccgtgc gcaccatgct gctgaaagtg agcagcattg acggcaatac
    15901 taaactggac gccctggata tcgattccaa ccaggggatc gtcaacgcca gcggcacggc
    15961 gcagctgtca gacaactggc cggtggatat caccctcaac agcacactga acgtggagcc
    16021 gttgaaaggt gaaaaagtga agctaaaagt gggcggcgcg ctgcgcgaac agctggagat
    16081 tggcgttaat ctttccggtc cggtggatat ggatttacgc gcccagacgc gactggcgga
    16141 agccggattg ccgctcaacg tggaagtgaa cagcaaacag ctttactggc cgttcactgg
    16201 tgagaagcag tatcaggcgg atgatctgaa actgaaactt accggcaaaa tgaccgatta
    16261 cacgctctct atgcgtacgg cagtgaaggg acaggagatc ccgccagcca ccattaccct
    16321 tgatgccaaa ggtaatgaac agcaggtcaa tctcgacaaa ctcaccgtcg cggcgctgga
    16381 agggaaaact gaactcaagg cgttgctcga ctggcagcag gccattagtt ggcgcggtga
    16441 gctaacgctt aatggcatta acaccgccaa agagttcccg gagtggccgt cgaaactcaa
    16501 tggcttgatt aaaacccgcg gtagcctgta cggcggcacc tggcagatgg aggtgccaga
    16561 actgaagctg accggtaacg tcaaacagaa caaagtgaac gttgacggca cgctgaaagg
    16621 caacagttat atgcagtgga tgatccctgg gcttcatctg gaactcgggc caaacagtgc
    16681 cgaagtgaaa ggcgagctgg gggtaaaaga tctcaatctt gatgccacca tcaacgcgcc
    16741 ggggctggat aacgcgctgc cggggcttgg cggtacagcg aaagggctgg tgaaagtacg
    16801 cggcacggtg gaagcgccac aactactggc ggacatcacc gcgcgcggcc tgcgctggca
    16861 ggaactttcc gtggcgcagg ttcgcgtgga aggcgacatt aaatccaccg atcagatcgc
    16921 cgggaaactc gacgtacgcg ttgagcaaat ttcgcagccg gatgtaaata tcaacctcgt
    16981 caccctgaat gccaaaggca gcgaaaagca gcacgagcta cagttgcgga ttcagggcga
    17041 gcctgtctcc gggcagctta atctggcagg aagttttgat cgcaaagaag aacgctggaa
    17101 gggaattctt agcaataccc gcttccagac gccggtcggc ccgtggtcgc tgacccgcga
    17161 tattgctctg gattaccgca ataaggagca aaaaatcagc atcgggccac actgttggct
    17221 taacccgaat gcggagctgt gcgtgccgca aactatcgat gcgggggccg aagggcgtgc
    17281 ggtggtgaat ctcaaccgct tcgacctcgc catgctgaaa ccgtttatgc cgcaaaccac
    17341 tcaggccagc ggtatcttca cgggtaaagc ggacgttgcc tgggacacca cgaaagaggg
    17401 gctgccgcag ggcagtatca ccctttcggg gcgtaacgtg caggtaacgc aaaccgtcaa
    17461 cgatgcggcg ctgcccgtgg cgtttcagac gctgaatctg acggcggaat tgcgtaacaa
    17521 ccgtgccgaa ttgggctgga ccatccgcct gaccaataac ggccagtttg atggtcaggt
    17581 gcaggtgacc gatccgcaag gccgccgtaa tcttggtggc aacgtcaata tccgtaactt
    17641 caaccttgcg atgataaacc ccatctttac ccgtggggaa aaagcagcgg ggatggtgag
    17701 cgccaacttg cgtctgggtg gtgatgtgca aagcccgcag ttgtttggtc agcttcaggt
    17761 tacgggtgtg gatatcgacg gcaactttat gccgtttgat atgcagccga gccagcttgc
    17821 ggtcaacttt aacggtatgc gctcgacgct tgccggtaca gtacggaccc agcagggtga
    17881 aatctacctg aacggtgatg ccgactggag ccaaattgaa aactggcggg cacgagtaac
    17941 ggcgaaaggc agtaaagtgc ggatcaccgt gccgccgatg gtacgaatgg atgtatcgcc
    18001 agatgttgta ttcgaggcta caccaaccct gtttaccctc gatggtcgcg tggatgtccc
    18061 gtgggcgcgc atcgtggtgc acgatctgcc ggaaagcgca gtaggcgtct ccagcgatgt
    18121 ggtgatgctt aacgataacc tgcaaccgga agagccgaaa acggcgtcga ttccgattaa
    18181 cagtaacctg attgtccacg ttggcaacaa tgtgcgcatt gacgcctttg gcctgaaagc
    18241 gcggctgacg ggcgatctta acgtcgttca ggacaaacaa gggctgggcc tgaacgggca
    18301 gatcaacatc cctcaagggc gcttccatgc ctatggtcag gatctgattg tgcgtaaggg
    18361 tgagctactg ttctctggtc cgccagatca accgtatctt aatattgaag ctattcgtaa
    18421 cccggatgct acagaagacg acgtaatcgc cggagttcgc gtcactggtc tggcggacga
    18481 accgaaagcg gagatcttct ctgacccggc gatgtcgcaa caagctgcat tgtcttattt
    18541 gctacgtgga caagggctgg agagcgatca gagcgacagt gcggcgatga cctcgatgct
    18601 gattggcctg gaggttgcac aaagtggtca gattgtgggt aaaatcggcg agacgtttgg
    18661 cgtaagcaat ttagcgctcg acaccgaggg agttggcgac tcctcccagg tggtggtcag
    18721 cggctatgta ttgccaggtc tgcaagtgaa atacggcgtg ggtatatttg actctatagc
    18781 aacactcacg ttacgttatc gcctgatgcc taagctatat ctggaagccg tgtctggtgt
    18841 agaccaggca ctggatttgc tctatcagtt cgagttttag caatgcgaat atttgtctac
    18901 ggcagtttac gccacaaaca aggcaacagt cactggatga ccaatgccca gttactgggc
    18961 gatttcagta tcgataacta ccagttgtat agcctgggcc actatccagg cgcagttccg
    19021 gggaacggaa cagtacacgg tgaagtttat cgtattgaca acgccacgct ggccgaactt
    19081 gatgccttgc gcaccagggg cggtgaatac gcgcgccagt tgattcagac gccgtacggg
    19141 agtgcatgga tgtacgttta tcaacgaccc gtcgatggat taaagctaat tgaaagcggc
    19201 gactggttag acagggataa gtaaccatat gcatacgcca ccttcgggtg gcgttgtttt
    19261 ttgcgagacg actcgcattc tgttttgtaa ttccctcacc ctttgctttt ctctccgagc
    19321 cgctttccat atctgttaac gcataaaaaa ctctgctggc gttcacaaat gcgcaggggt
    19381 aaaacgtttc ctgtagcacc gtgagttata ctttgtataa cttaaggagg tgcagatgcg
    19441 tattaccata aaaagatggg ggaacagttc aggtatggtc attcctaatg ttgtgatgaa
    19501 agaacttaac ttacggccgg ggcagagcgt ggaggcgcaa gtgagcaaca atcaactgat
    19561 tctgacaccc atctccaggc gctactcgct tgatgaactg ctggcacagt gtgacatgaa
    19621 cgccgcggaa cttagcgagc aggatgtctg gggtaaatcc acccctgcgg gtgacgaaat
    19681 atggtaaaga aaagtgaatt tgaacgggga gacattgtgc tggttggctt tgatccagca
    19741 agcggccatg aacagcaagg tgctggtcga cctgcgcttg tgctctccgt tcaagccttt
    19801 aatcaactgg gaatgacgct ggtggccccc attacgcagg gcggaaattt tgcccgttat
    19861 gccggattta gcgttccttt acattgcgaa gaaggcgatg tgcacggcgt ggtgcttgtg
    19921 aatcaggtgc ggatgatgga tctacgcgcc cggctggcaa agcgtattgg tctggctgcg
    19981 gatgaggtgg tggaagaggc gttattacgc ttgcaggcgg tggtggaata aggtgtgttt
    20041 atttatcgcg ggcataaaaa aacccttact aaccgaagcc cggtgttcag ggttattgcg
    20101 ccagaagaac ttatttattc tttgcgcgct cgaaggaggc aacgatttca gctttagcgg
    20161 cttctgcgtt ttcccaacct tcaactttca cccacttgcc tttttcgagg tctttgtagt
    20221 gctcgaagaa gtgagcgatc tgcgctttca gcagctctgg cagatcgtta acgtctttaa
    20281 tgtgatcgta ttctttgctc agcttggtgt gcggaacagc aatcagtttc gcatcttcac
    20341 cggcttcgtc ggtcattttc agaacgccaa ccggacggca acggatcaca gaacccggct
    20401 gcagcgggta cggagtcggg accagtacgt caaccgggtc accgtccaga gacagggtgt
    20461 ggttgatgta accgtagttg cacggataga acatcgcggt ggacatgaag cggtcaacga
    20521 acagtgcgcc gctctctttg tcgatttcgt atttgatcgg atctgcgtta gccgggatct
    20581 caataaccac gtagatgtct tccggcagat ctttacccgc agggacgttg agtaagctca
    20641 tgtctgtttc ctttaaaaat atgtggcaaa caagtgccga gtattatagc caactcgcgc
    20701 cgaatgtctt cgcttgtttt cgtcttcatt ttcctttttc gccagtttta agacggtatg
    20761 cagagcagga aaatccataa ccgtagccgc atttttcata gtgagatgaa agcgattaca
    20821 aacttgtgat taacgtttta tttacttttt tgaagtgtga tgtaacgcaa tccgttacat
    20881 aacgaattgt ctatagtttt ttcgcgaaca tcttttcacc aataataatt accccgacga
    20941 ggacaaccct atgtggaaac gcttacttgt agtctctgca gtctcggcag ccatgtcgtc
    21001 tatggcgttg gccgctccat taaccgtagg attttcgcag gtcggatcgg aatccggctg
    21061 gcgtgccgca gaaaccaatg tggcgaaaag tgaggccgaa aaacgcggaa ttacgttgaa
    21121 aattgccgat ggtcagcaaa agcaggaaaa ccagattaaa gcggtacgtt ccttcgttgc
    21181 acaaggggtg gatgcgatct ttatcgctcc agtggttgcg acgggttggg aaccggtatt
    21241 aaaagaggcg aaagatgccg aaatcccggt cttcttgctc gaccgttcca tcgatgtgaa
    21301 agacaaatct ctctatatga ccaccgttac tgccgacaac atcctcgaag gtaagttgat
    21361 cggtgactgg ctggtaaaag aagtgaatgg caaaccatgc aacgtggtgg agctgcaggg
    21421 caccgttggg gccagcgtcg ccattgaccg caagaaaggc tttgccgaag ccattaagaa
    21481 tgcgccaaat atcaaaatta tccgctcgca gtcaggtgac tttacccgca gtaaaggcaa
    21541 agaagtcatg gaaagcttta tcaaagcgga aaacaacggc aaaaacatct gcatggttta
    21601 cgcccataac gatgacatgg tgattggcgc aattcaggca attaaagaag cgggcctgaa
    21661 accgggcaaa gatatcctca cgggttccat tgacggtgta ccggacatct acaaagcgat
    21721 gattgatggc gaagcgaacg ccagtgttga actgacgccg aatatggcgg gacccgcttt
    21781 tgacgcgctg gagaaataca aaaaagacgg caccatgcct gaaaagctga cgttaaccaa
    21841 atccaccctt tacctgcctg ataccgcaaa agaagagtta gagaagaaga aaaatatggg
    21901 gtattgaggg ttgctatgcc tgatgccgat tcgtaggccg gataaggcgc tcgcgccgca
    21961 tccggcggtg gtgcgctgaa gcctgatgcg acgcttaccg cgtcttatca tgcctactgg
    22021 gagcacgctt tacaccgggg gaaaccatga cgactaacca acaccaggag atcctccgca
    22081 ccgaaggatt aagtaaattt ttccccggcg tcaaagcgtt agacaacgtt gatttcagcc
    22141 tgcgccgtgg cgaaatcatg gcgctgctcg gtgaaaacgg ggcgggaaaa tcgacgctaa
    22201 tcaaagcatt aaccggtgta taccacgccg atcgcggcac catctggctg gaaggccagg
    22261 ctatctcacc gaaaaatacc gcccatgcac aacaactcgg catcggaacc gtttatcagg
    22321 aagtcaacct gctacccaat atgtcggtcg ctgataatct attcataggt cgcgaaccca
    22381 aacgtttcgg ctttctacgc cgcaaagaga tggaaaagcg tgccaccgaa ctgatgacat
    22441 cttacggttt ctccctcgac gtgcgtgaac cgcttaaccg tttttcggtg gcgatgcagc
    22501 aaatcgtcgc catttgccgg gctatcgatc tctccgccaa agtgctgatc ctcgatgaac
    22561 ccaccgccag cctcgacacc caggaagtgg agctattatt tggcctgatg cgtcagttgc
    22621 gcgatcgcgg cgtcagcctg atctttgtca ctcactttct cgatcaggtc tatcaggtca
    22681 gcgatcggat cactgtctta cgcaacggca gtttcgtagg ctgtcgggaa acgcgcgagc
    22741 taccgcagat cgaactggta aaaatgatgc tggggcgcga gctggatacc cacgcgctac
    22801 agcgtgccgg gcgaacattg ttgagcgaca aacccgttgc cgcgttcaaa aattacggca
    22861 aaaaaggaac gatagcaccg tttgatctcg aagtgcgccc cggcgagatc gtcggtctgg
    22921 ctggcttgct gggatccgga cgtaccgaaa ccgccgaagt gatcttcggt atcaaacctg
    22981 ctgacagcgg cacggcgttg atcaaaggca aaccgcaaac cctgcgatcg ccacatcagg
    23041 cttcagtact gggcatcgga ttctgcccgg aagacaggaa aaccgatggc atcatcgccg
    23101 ccgcctcggt gcgggaaaat atcatcctcg ctctccaggc ccagcgcggc tggctacgac
    23161 ccatttcccg caaagagcag caagagattg ccgaacgctt tatccgccag cttggcattc
    23221 gcacaccttc aactgaacaa ccgattgaat ttctctccgg tggcaatcag caaaaagtgc
    23281 tgctttcacg ttggctactg acccgaccgc aatttctgat ccttgatgag ccaacccgtg
    23341 gcattgatgt tggtgcccac gccgagatca tccgcctgat tgaaacgcta tgcgccgatg
    23401 gtctggcgct gctggtgatc tcctccgaac tggaagaact ggtgggctat gccgaccggg
    23461 tgatcatcat gcgcgatcgc aaacaggtgg cggagatccc gctggcagag ctttccgttc
    23521 cggcgatcat gaacgccatt gcggcgtaag gagaacagtg tgatgcctca atctcttccg
    23581 gacaccacta cgccaaaaag gcgctttcgc tggccgacgg ggatgccgca gctggtagca
    23641 ctattgctgg tgctgctggt cgacagcctg gtagccccgc atttctggca ggtagtgctt
    23701 caggatgggc gtttgttcgg tagccccata gacattctta accgtgcagc ccccgttgcg
    23761 ttactggcga ttggtatgac gctggtgatc gccaccggtg ggattgatct ctccgtaggg
    23821 gcggtgatgg ctatcgccgg agccacaacg gctgcgatga cggtcgcggg agtcagcctg
    23881 ccgattgttt tgttaagcgc cctgggcacc ggcatcctgg cgggattgtg gaacggcata
    23941 ctggtagcga tcctcaaaat tcagccattt gtcgccaccc taatcctgat ggtcgccggg
    24001 cgcggcgtgg cgcaactgat cacctccggc cagatcgtca cgtttaactc gccggatctc
    24061 tcatggtttg gcagcggatc gctgttgttc ctgccaacgc cggtcattat cgcggtgctg
    24121 acgcttctcc tgttctggct gttgacccgc aaaacggcgc tggggatgtt tatcgaagcc
    24181 gttggtatca acattcgggc ggcaaaaaat gccggggtaa acacgcgaat catcgtcatg
    24241 ctcacctacg tgttgagcgg gctatgtgcg gcgattgcgg gcattatcgt ggcggcggat
    24301 attcgcggtg ccgatgccaa caacgccgga ttatggctgg agctggacgc cattctcgcg
    24361 gtggtgattg gcggcggatc gctgatgggc gggcgcttta acctgctgct ctcggtagtg
    24421 ggggcgctga ttattcaggg gatgaacacc ggaattttgc tttcgggctt tccaccggag
    24481 atgaaccagg tggtgaaagc ggtggtggtg ctttgcgtgc tgattgtcca gtcgcaacgc
    24541 tttatcagtc tgattaaagg agtacgtagc cgtgataaaa cgtaatttgc cactgatgat
    24601 caccatcggc gtctttgtgc tgggctattt ctactgcctg acgcagtttc ccggttttgc
    24661 ttccacaaga gtgatctgca atatcctgac cgataacgct tttctgggga ttattgccgt
    24721 tggtatgacc tttgtgatcc tctctggcgg tatcgatctc tccgtcggtt cggtgatcgc
    24781 ttttaccggc gtgtttctgg caaaagtgat tggcgacttc ggcctctcgc cgttgctggc
    24841 atttccgctg gtgctggtaa tgggctgtgc ctttggcgca tttatggggc ttctgatcga
    24901 cgccctgaag atcccggcat ttatcattac gcttgcgggg atgttctttt tgcgcggcgt
    24961 cagctatctc gtttcggaag agtcgattcc gataaaccat cccatttatg acacgctctc
    25021 aagccttgcg tggaaaatcc ctggcggcgg tcgcttaagt gcgatggggc tgctgatgct
    25081 ggcggtggtg gttatcggca tattcctcgc gcatcgtacc cgttttggta atcaggtata
    25141 cgccattggc ggcaacgcaa cgtcggcaaa cctgatgggg atttctaccc gcagcaccac
    25201 tattcgcatt tatatgctct ccaccggact ggcaacgctg gcggggattg tcttctcgat
    25261 ttatacccag gccggatatg cgctggcggg cgtaggtgtg gaactggacg ctatcgcctc
    25321 agtggtgatt ggcggtacgc ttttgagcgg tggcgttgga acggtattag ggacgctttt
    25381 tggcgtggcg attcagggac tgattcagac ttacataaac tttgatggca cgctgagttc
    25441 ctggtggacg aaaatcgcca tcggtatttt gttgtttatt tttatagcat tacagcgtgg
    25501 attaacggtg ctgtgggaga atcgtcagag ttcgccagtg acaagagtca acattgcgca
    25561 gcaataaaaa cgcctctccg tgtggagagg cgcaggagat tacgcgtccg ggaactcacg
    25621 gataaagcgt tcgacatctt caaccatatg gtcgttgccg acaaagaacg aacggcgctg
    25681 gtgcagggtt tccgggataa tatccagaat acgctctttg ccatcgctcg ctttaccgcc
    25741 cgcttgttcc gccaggaatg ccatcgggtt gcactcatac agtaaacgca gtttaccgtc
    25801 cgggtggctg gcggtgcttg ggtagagata aataccgcct ttcagcaggt tacggtggaa
    25861 atccgcaacc agtgaaccga tataacgtga ggtatacggg cggttggtgg atttatcttc
    25921 ttcctggcag aatttaatgt acttcttcac cccgttcgga aacttaatgt agtttccttc
    25981 gttgatggag taggttttgc ctttctccgg gaagcgcatc cgttcctggc acaggcagaa
    26041 aacgcccagc gaaggatcgt aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag
    26101 catagtagag gagccgtata ccacgtaacc tgccgcaacc tgtttgttac caggctggag
    26161 gaaatcttct tccgttaccg gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat
    26221 ggtaccgaca gagacgttaa catcgatgtt ggacgagcca tccagggggt ccatcagcac
    26281 cacgtatttt gcgtgttcac agccttcaaa gacgacaatc tcatcttctt cttcagaggc
    26341 aatgcccgca acgatatcgc gtgctttcag tgcggctttc agtttttcat tagcgaacaa
    26401 gtcgagtttc tgctgaacct cgccctgcac gttctcagca ccgctggcac ccaggatatc
    26461 aaccagtcct gctttgttga tatcgcgatg gataatcttg gcgcccagtt ttattgccga
    26521 cagcaaagca gtgagctcac cggtagcatg agaaaactcg tgctgctttt cgacaataaa
    26581 ttcacctaac gttttcataa aactttccct gcaatgttta tggagtaaag cgaccgcaac
    26641 aatcttaaca aataatctca atgttgcgct caggtgaatc gcgccagcaa attgcggatt
    26701 atcctgaaat gcgtttctca cttgcccgac atatgcgtaa aatgagcggc agattaaaaa
    26761 aggatagtga cgtatgcgca ttcatatttt aggaatttgt ggcacgttta tgggcggtct
    26821 ggcgatgctg gcgcgccagt taggccatga agtaacgggt tcggacgcca atgtgtatcc
    26881 gccgatgagc accttacttg agaagcaagg cattgagctg attcagggtt acgatgccag
    26941 ccagctcgat ccgcagccgg atctggtgat tattggcaac gccatgaccc gtggaaatcc
    27001 gtgtgtggaa gcggtactgg aaaaaaacat cccttatatg tcaggtccac agtggctgca
    27061 cgattttgtg ctgcgcgacc gctgggtgct ggccgttgca ggtacacacg gcaaaaccac
    27121 caccgcggga atggcgacct ggattctgga acagtgtggt tacaaaccgg gctttgtaat
    27181 cggcggtgtg ccggggaact ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt
    27241 tatcgaagcg gatgagtacg actgcgcctt cttcgacaaa cgctctaaat ttgtccatta
    27301 ctgcccgcgt acgctgatcc tcaacaacct tgagttcgat cacgccgata tctttgacga
    27361 cctgaaagcg atccagaaac agttccacca tctggtgcgt atcgttccgg ggcagggccg
    27421 tattatctgg ccggaaaacg acatcaacct gaaacagacc atggcgatgg gctgctggag
    27481 cgagcaggag ctggtgggcg agcaaggtca ctggcaggcg aaaaagctga ccactgacgc
    27541 ttctgaatgg gaagtcttgc tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt
    27601 gggcgaacat aatatgcaca atggcctgat ggcgattgcg gtggctcgcc atgttggtgt
    27661 agcgcctgca gatgccgcta acgcgctggg ttcgtttatt aacgctcgtc gccgtctgga
    27721 gttgcgtggt gaagcgaatg gcgtgacggt atatgacgat tttgcccatc acccgacggc
    27781 aattctggca acgcttgcgg cgctgcgtgg caaagttggt ggtacggcgc gcattattgc
    27841 tgtgctggag ccgcgctcga ataccatgaa aatggggatc tgcaaagacg atctggcacc
    27901 ttcattaggt cgtgccgatg aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt
    27961 ggcagaagtg gcagaagcct gcgttcagcc tgcacactgg agtggcgatg tggatacgct
    28021 ggcagatatg gtggtgaaaa ccgctcagcc tggcgaccat attctggtca tgagcaacgg
    28081 cggttttggt gggatccatc agaaactgct ggatggtctg gcgaagaagg cggaagctgc
    28141 gcagtaattc ggcctcagcc tgagatagca ttgccggata aggcgtttac gccgcatccg
    28201 gcaaaccatt ctcacatcaa caacgaatgt tacccttcgt tttccgctaa ctcgcgcaga
    28261 tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc tttcttcgca
    28321 ttgcggatca gagtacgcag ctgctgacga tcggcatcgg gccacagatt caatacttca
    28381 gcgagagcgt catctccctg gtcgatcaga cgatcgcgca ggttttccag tttatggaac
    28441 agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg gatcggttca
    28501 acatcgcgct ggcgcagcat cttaccaatg agctgcaact ggcggcggcg accttccatc
    28561 ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg gatcttatcc
    28621 agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc ctcggcatcg
    28681 cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc gatgtcatca
    28741 ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa
    28801 gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta gacttcagag
    28861 aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca
    28921 agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa tcggacggtg
    28981 cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat ggtgaagtgg
    29041 agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac cagaaccgca
    29101 aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact gtacaggcgg
    29161 cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca gacaaagagc
    29221 tgctggcctt tgacgcacca gatctcgact tgttccaccc tgcggatgtt tccccggatg
    29281 aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac aaacgcatca
    29341 ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt ggcaacagcc
    29401 acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt gtaattgccg
    29461 aagaaaatgg cgatatggag cgtgattacg cctacaccat tggccgcgcg atgagcgatc
    29521 tgcaaacgcc agagtgggtc ggggccgact gtgctcgccg tactttatcg cgcctgtcac
    29581 cgcgtaaact ctccaccatg aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc
    29641 tttttggcca tctggtgggg gcgatagcgg gtggatcggt ttaccgtaaa tctaccttcc
    29701 tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa gagcatccgc
    29761 atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc
    29821 gcgatattat taaagatggc atcctgactc agtggctgct gaccagctat tcggcgcgga
    29881 aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg attgccggac
    29941 aagggctaag cttcgaacaa atgctcaaag agatgggcac cgggctggtg gtgacggagc
    30001 tgatgggcca gggcgtgagt gccattactg gtgattattc ccgtggtgct gcgggcttct
    30061 gggtagagaa tggcgaaatt cagtatccgg ttagtgaaat caccatcgca ggtaatttaa
    30121 aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac
    30181 agtgtggttc tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa actggcgcga
    30241 cctgccgcgc tataaactaa aaaattacac aaaatcattc gcactgcatc gagacggaaa
    30301 gtgaatgaga gccggggagc gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt
    30361 cgatgaggca gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag
    30421 cctgttagct attcttgcag tctcctcgtt ggtattcagt tcggcgtcat ttgctgctga
    30481 tctggaagac aatatggaaa tcctcaacga caatttaaaa gtggtcgaaa aagcggataa
    30541 cgcgtcgcaa gtcaaagacg cgttaactaa aatgcgcgct gcggcgctgg atgcgcaaaa
    30601 agcaacgccg ccgaagctgg aaggcaaatc accggacagc ccggagatga aagacttccg
    30661 ccatggtttc gatattctgg tcggtcaaat tgacgacgcg ctgaagctgg caaatgaagg
    30721 taaagtaaaa gaagcgcagg ccgccgcaga gcaactgaaa acgacccgca acgcctatca
    30781 ccagaagtat cgttaattcc tcatttccct gttgcctgca ctcaggtaac agggaactta
    30841 tcatcgcaaa tgatgcacca cctgattgct gctgccgcgc cagatcaggg aagggtcttt
    30901 taaatcctgc acaaacttgc cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg
    30961 cgcagcgttg agttcgtcga gtttatagcc tgtccacacc cagatgtctt tacccggaca
    31021 ctcggcgcgg atgcgtttta ccagtttcag aatatccggc acgttttgcg gatgcagcgg
    31081 atcgccgccg gagagggaaa tcccctggcg tttgatacga gtgtcattca gatctttaat
    31141 gatcttgtct tccattgctt tggtaaatgg ctgaccagaa tttacccgcc atgtgctttt
    31201 gttatagcaa ccggggcatt catgaacaca cccggagaca aacagggtgc agcgagtgcc
    31261 ggggccgttg acgatgtcga caggatagta ctgatgataa ttcattattt cgcctggctt
    31321 atcagtccgc gtgtaggctg catccggtaa tcaatgcctg atgcgacgct gtcgcgtctt
    31381 atcaggccta cagccgttgc caaatgtagg ccggataagg cgtttacgcc gcatccggca
    31441 tttgcttagc gcagagtgaa gattaaccta tctgcccatt ccccaaatgt ttaacgcggc
    31501 gcttaacttc ttcctgctta ccagcgttaa acggacgtgc atccgggcta cctaaatatc
    31561 cgcacacgcg gcgagttacc gacacacggg aggcgtcatg gttaccacat ttcgggcaag
    31621 tgaagccttt gctggtgcac tcgaactcac cggtaaagcc acactcgtag cactcatcaa
    31681 tcggtgtatt ggtgccgtaa tacggtacat gctgatagct gtaatcccag acatcttcca
    31741 gcgctttcag gttgtgctga atgtttggat actcgccgta gcaaatgaaa ccaccgttcg
    31801 ccagcggcgg gtaaggcgct tcaaagtcga tcttgtcgta cgggttcacc ttcttctcca
    31861 catcaaggtg gaaactgttg gtgtagtaac ctttatcggt cacgcccggc accacgccaa
    31921 actcagcagt atcgagacgg cagaagcggt cgcacaggtt ttcactcggc gtgctgtaga
    31981 gactgaaacc ataacccgtt tcttctttcc actgatccac tgcctgacgc agacgttcaa
    32041 caatggcgat acctttcgcg cgaagctgct cgttgtcgta aacatgctcg ccgccgaaca
    32101 gcgcgttaat ggtttcgtgg atgccgatgt aacccagcga aatagacgca cgaccgtttt
    32161 tgaagatttc agaaacatca tcgtcagcat tcagacgtac gccacaagca ccttccatat
    32221 agaggatcgg ggcgacgcgc gctttcacgc cttcgagacg agcgatacgg gtcatcagtg
    32281 ccttacgtgc cagcaccaga cgttcatcca gcagcttcca gaaggtggct tcatcgcctt
    32341 ttgcttccag agcaatacgc ggcaggttca ggctgatcac gccgaggttg ttacgaccat
    32401 cgtggatctg ctcgccgttt tcattttccc acacgccgag gaagctgcgg cagcccatcg
    32461 gggttttaaa cgaaccggtg actttcacta cctgatcgta gttcaggata tccggataca
    32521 tgcgcttgct tgcgcactcc agcgccagct gtttgatgtc gtagttcgga tcgccttttt
    32581 tatggttcag accatcgcga atcgcaaaca ccagtttcgg gaacaccgca gttttacggt
    32641 ttttaccgag gcctgcgata cggttacgca ggatcgattc ctgaatcagg cgcgattccc
    32701 agctggtgcc cagaccaaaa ccaaaggtta caaacggcgt ctgaccgttg gcggtgtgca
    32761 gggtgtttac ttcgtactcc agcgactgga aggcatcgta gcactctttg atggtacgag
    32821 agttagcgta gccttcggcg tccgggatgt tccactcttc tgcggtcttg cgatgtttgt
    32881 tgtagctggc ggtaacaaac ggcgccagca cttcatcgat acggttaatg gtggtgccgc
    32941 cataaatatg gctggcgacc tgagcaataa tctgcgcagt taccgcggtt gccgtagaga
    33001 tcgacttcgg cggttcaatc tcggcgttcc ccattttaaa gccctgggtc agcatgcctt
    33061 tcaggtcgat caacatgcag ttgaacatcg ggaagaacgg cgagtaatcg agatcgtgat
    33121 agtgaatatc gccacgctca tgtgcctgca ccacgtcacg cggcagcagg tgctggcgtg
    33181 catagtgttt agccacgatc ccggccagca ggtcgcgctg ggttggaatc accttgctgt
    33241 ctttgttggc gttttcgttg agtaacgagg cgttggtctg ctcgaccaga ccacggatct
    33301 cctggttcag gcgaccgcgt ttttcacgtt caatgtcgcg atcgtgacgg tactcgatgt
    33361 aagcacgagc cagttgtttg tatggacccg acatcagctg gttttcaact gcggtctgga
    33421 tctcgttgat atccacctgg ttgcggccct gcatctgctc gctgacaacc gcggcaacag
    33481 tggcgcaata atcggcatca tcgacttccg ctgctttagc tgcacgcaga atcgcttctt
    33541 tgatgcgctc tgatttaaac ggcactttgc agccgtctcg tttcatcaca tgcggtgtca
    33601 tgatcgctcc atatttttaa gaacaggtta tccacagaaa ttgggaaagg cgttttccgg
    33661 ttttttcgtt tcgttttccg gtgctttccg caatcgccat ccgcttatcc acaacgccgc
    33721 acctttttcg tggggagctg tagtagcaat tatagttgat taatacaaca tattgggttg
    33781 ggacgcattt taaagtctat atatagtgct ttgcatcaag gatgtttgaa ctttttttga
    33841 tgtagctcaa agtaaaaagc agagcgtacg gatgacgggc gctacagcgt tatgtaaatt
    33901 ttttaatgaa tttgctggtt gaaaaatcaa caaaaacaac atgctgacag acaaaacccc
    33961 ggaatgacag gcattcccgg ggcttagcgg atacttactt ctgtaaccac cagacagcct
    34021 caaaaggccg taaagtcatg gcacagggtt gtggtgaggc ttcttcgtag ttatgcatca
    34081 caagctgcca gttgccgcgc atttgccctg gctgccaggg ttggatctca cggctaaggt
    34141 tggcaatgac cagcaaggtt tgccccttcc attcacggcg atagcaccac aatacagggc
    34201 tgtttggcag cagatcctgg taattgcccc atgtcaggat ggcttcttgc ttacgcagtg
    34261 cgattaactt ttggtaggtg taaaacaccg aggagtcatc ggccagcgcg gcttctacgt
    34321 tgatttgttg atagttatcg cccaggccaa tccacggttc gccagccgta aacccggcat
    34381 tatcgccgtt gctccattgc atgggcgtgc ggctgttgtc acgggattta ctggcgagga
    34441 ttgccaataa ctcgtcggca tcacgaccat cgttgcgcag ctcggcaaac atattgaggc
    34501 tctccacgtc gcgatagtca gtaatgcgcg tgaaatgcgg gttggtcatg ccaatctctt
    34561 cgccctggta gatatacggc gttccctgca tgccatgcag caccatcgcc agcatttttg
    34621 ccgcaggcac gcggtattca ccttcatcac caaagcgaga aacaatgcgc ggctgatcgt
    34681 ggttacacca gaacaaggca ttccatgcca cgttgtgcat tccttgttgc cagtggcgga
    34741 acaatgtttt caacgccaca aagtcaggtt tagccagcgt ccatttttca ccaccgggat
    34801 aatcgacctt caggtgatga aaattaaagg tcatcgacaa ttcactgcct gtcagtgccg
    34861 cgtatcgctg gcaatgctca aggctggtgg aggacatttc acctacggtc attaacccgc
    34921 gtggcgtaaa cacatcgcgg ttcatctcgt gcaaaaactc gtgtgctcgt ggcccgtcgg
    34981 tgtagaagcg acgcccgtcg ccatccaggt cttcagggaa acgcgggtct ttggagatca
    35041 gattcaccac atccaggcgc aacccgtcga ccccacgatc ggcccagaat tcacagactt
    35101 ttttcagctc tgcgcgtacc gctggattct cccagttgag atccgcctgt tctggtgcaa
    35161 agagatgcaa atagtactgt tcgctttccg catgccagcg ccacgcacta ccgccaaatt
    35221 ttgaacgcca gttgttcggt ggcgtttctg gttcgccatc gcgccagata taaaactggc
    35281 ggtaagggct ttctttgttc agcgcctcgc gaaaccaggc atgttgggta gaggtatggt
    35341 taaacaccat atcgagaatg atacgaatcc cgcgcgattt tgcctgcgtc accagttcgt
    35401 caaaatcgtc cagcgtgccg tagatgggat caatcgccgt atagttcgct acgtcgtaac
    35461 cgttatcgac ctggggagag atataaaagg gggttagcca gatggcatca acgcccagtt
    35521 tatgcagata gtccaggcgt tggataacgc cacgtaaatc gccggtgccg ctacccgtgg
    35581 tgtcctgaaa actctttgga taaatctggt agataacgcc gttttgccac cagtggggaa
    35641 gattagtcat tacgttattc ctgcaaatgc gagggggcgc aattgcgccc cgaagaaaat
    35701 taaacaatat ccagcgtgcc caggcggtat ttccgctgat agataaacga ggtgagtaca
    35761 atcgggatga tgatggcgat aaccattgcc agcgcaaata cctgccagta gctcggttga
    35821 atcgagagaa ttcccggcag gccgcctacg ccgataccat tcgccataac gccgttcagg
    35881 ccgcatagta atcctgccag accagaacca atcatcgcgc acagcatcgg gaagcgatat
    35941 ttcaggttga tgccgtacat tgcaggctca gtgaccccaa gccaggcgga gatagcggca
    36001 ggcacggaga tctcgcgttc attgtgcttg cggctggaaa tgataatgcc tatcacggcg
    36061 gagccctgag cgatattcga cagcgcaatc agcggccaca ctggcgtgcc acccatgctt
    36121 tgaatcatct gcaaatcaat agcaagggtg gtctggtgta cgccggtgat caccagcggc
    36181 gcgtacagga agccaaacaa tgccgcacca atcggagcaa agctgccggt catcaggtga
    36241 cgtaccgcaa aggcaacgcc atcgccaatc atgcgaccaa acggaccaat cagcgcatgg
    36301 gcgaggaaca ccgcgaggat cagcgaacag acgggcacca ccaccagata gaggtaatcc
    36361 ggtacgatgc gtttaaggcg agtttcaata acgcccagtg ccagcccggc taacagtgcc
    36421 gggatcacct gtgcctgata gcccactttg gcgatgctga acatgccaaa gtcccacact
    36481 tccggcagct gctgcccgag cagataagcg ttcatcagct ggggagaaac cagtgtcacg
    36541 ccaagcacga taccaaggat cggcgtgccg cccatttttt tcaccgctga ccagcaaata
    36601 ccgaccggca ggtagaagaa gatcgcttca ccgatcaacc acagaaaatc gtagatcgtt
    36661 tgcagggaag ggtacatttg cgccagcgtc tgaccgttgc tcatgggcaa atcgccgatc
    36721 acattgcgaa aaccgaggat caaaccgccg ctaatcaacg cgggcagcaa cgggaagaag
    36781 atctccgcga aatgagagat caactgctca tgccatttca tattctgccg ggcggctttt
    36841 tttacctgct ctttatcaac ctgcgcctgt ccggttgacg ctattagtgc ttgatagtaa
    36901 tcacccacgt tggtgccaat caccacctga aattgcccgg cattggtgaa acagcctttc
    36961 accatgggga gttgctcaat ttctttcggt ctggcattgg ccggttggtt gaggacaaag
    37021 cgcaggcgag taatacagtg gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca
    37081 atcaaccgat cgatatccgt ttggtttatt ttgctcatca taaagcccca tggcagatga
    37141 catttttggt tggctgcaga atatattgcg cggaaaaatt taaaaacggg aacgttcccg
    37201 aaacgcagcg aagatcacaa tttatcgttc aggaaacgat caggacaggg tggcggggat
    37261 gatgatttgt tgcggttcgc tgcgcccggt tacctgtgcg atcaattggc aggccgcctg
    37321 gcgtccagct tcggcgtaac caggatctac ggtgacgatc tccggatgaa ggaatttcat
    37381 taatggcgta ttaccgacgc tcgccagttg caaggtgtcg atgcgttgct cttgcaggta
    37441 tttacttgcg ccaagtgcca gcgtgtcggt tgcgcacagt aaggcggtag tttcaggcgt
    37501 aatcactttt gctacgttct catagccttg cttcatagca aggcctggca gagcggcaac
    37561 gggatgcagt ttatgcgctt tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt
    37621 cgtcacgtca ctatgcggca cgccgagata actgatatta cgatgcccct ggtcatacag
    37681 ccgttgcatc aggatcttga ttgccccttc gtcgtcataa cagaccgaag caaaaccttt
    37741 tgcgtcacgc gccagcagaa ccagcgatga ctgcaagtgg gctaacattt cttctgttat
    37801 gccagtaaaa ccgaacagca ctacgccgtc gatattacgc cgtttcagca cccccaaatg
    37861 ttcggcaact aattgcgggg aaaactgact ttccatcatg attgggtcgt aaccttgttc
    37921 atagaacgct ggcagcatgg tttgaacggc gagattttct gacaacgaat ccaggcgggt
    37981 aacaatgatg gcgaccactt tatcgctttg cccacgcata gcgcgcgcag agcgggaagg
    38041 ggaaaatcca tgctgattca tcactgcttc aacacgctcg cgggtgcgct ggctcacgcc
    38101 gctttcgtta ttcagcaccc gggaaactgt agatttcccc acgccgctta agcgtgcgat
    38161 gtctttgatg gtcagccgat tttgcatcct gttgtcctgt aacgtgttgt ttaattattt
    38221 gagcctaact ttacccgtgc attcagcaat gggtaaagtc tggtttatcg ttggtttagt
    38281 tgccagcagg tattatatcg ccatagatgc tacgaatatt attggattct ccttattatt
    38341 tgcggcgctt tattcactta ccggaggtta tatggaacct gatcccacgc ctctccctcg
    38401 acggagatta aaacttttcc ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg
    38461 ccgtgacgtt ttaacgtccc tgctcagctt tattaccttc aggtaaggct tcgccacgcc
    38521 tgaagacatt tctgtactgt ttcagacagt gcggagggac tccttatgtt taaagaaatc
    38581 tttacccggc tcattcgcca tttaccttcc cgtctggttc atcgtgatcc attgcctggc
    38641 gcgcagcaga cagtgaatac ggtggtcccg ccgtccttaa gtgcgcattg cctgaaaatg
    38701 gcggtgatgc ccgaagaaga attgtggaaa acgttcgaca cccatccgga agggttaaat
    38761 caggcggaag tggaatctgc ccgcgaacaa catggtgaaa ataaattacc cgcacaacaa
    38821 ccgtcgccgt ggtgggtaca tttatgggtc tgctatcgca acccatttaa tattttactc
    38881 accattctcg gcgctatttc ttacgccacg gaagatttat ttgccgcggg cgttatcgcg
    38941 ctaatggtcg ctatttctac gttgctgaac tttattcagg aagcacgttc cactaaagcg
    39001 gcagatgccc tgaaagcgat ggtcagcaat accgcgacgg tgctgcgcgt aattaacgac
    39061 aaaggcgaaa atggcaggct ggagatcccg atcgaccagc tggtgcccgg cgatattata
    39121 aaactggcgg cgggggatat gatcccggca gatttacgta tcttgcaggc gcgggatctg
    39181 ttcgtcgctc aggcgtcgtt aaccggtgag tctctgcccg tggaaaaagc cgctaccact
    39241 cgccagccgg agcacagcaa tccgctggag tgtgacaccc tgtgttttat gggcaccacc
    39301 gtggtgagcg gcacggcaca agcaatggtg attgctacag gagccaatac ctggtttggt
    39361 caactggcgg ggcgtgttag tgagcaggaa agcgagccga atgcctttca gcaggggatc
    39421 agccgcgtca gtatgctgct gattcgcttt atgctggtga tggctccggt ggtgttgctt
    39481 atcaacggtt acaccaaagg cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg
    39541 gtaggcttaa cgccggaaat gttgccgatg attgttacct cgacgctggc gcgcggcgca
    39601 gtaaagctgt cgaaacagaa agtgatcgtc aaacatctgg atgctattca gaactttggc
    39661 gcaatggata ttctgtgcac tgataaaact ggcaccctga cacaggacaa aattgtgctg
    39721 gagaatcata ccgatatctc cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg
    39781 aacagtcatt atcagaccgg acttaaaaac ctgctcgata cagcggtgct cgaaggtacg
    39841 gatgaagagt cagcgcgctc gctggccagt cgttggcaaa aaattgatga gattccgttt
    39901 gatttcgagc gtcgtcggat gtcggtggta gtggcagaaa ataccgagca ccatcagctg
    39961 gtttgcaaag gtgcattgca ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc
    40021 gagattgtgc cgctcgatga cattatgctg cgtaagatta agcgggttac tgatacgctg
    40081 aatcgtcagg ggctgcgcgt ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga
    40141 gattaccagc gggcggatga atccgacctg atcctcgaag gatatattgc tttccttgat
    40201 ccgccaaaag agacaaccgc tccggcactg aaggcattaa aagcgagtgg aatcaccgta
    40261 aaaatcctta ctggcgacag tgagttagtt gcagcgaaag tgtgccatga agtggggctg
    40321 gacgcgggag aggtggtcat tggtagtgat attgaaacgc tatctgacga cgaactggca
    40381 aatctcgcac agcgcaccac gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt
    40441 gtgaccttac tgaagcgcga agggcatgtg gttggcttta tgggcgatgg tattaatgat
    40501 gcgcccgcct tacgcgctgc ggatatcggc atttctgtgg acggcgcggt agatattgcc
    40561 cgtgaagcgg ctgatatcat cctgctggaa aaaagcctga tggtgctgga agagggggtt
    40621 attgaaggac gtcgcacttt cgccaacatg ctgaaataca tcaaaatgac ggcgagctcc
    40681 aacttcggta atgtgttcag cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg
    40741 ttgccgttac acttgcttat tcagaacctg ctgtatgatg tgtcacaggt ggcgatcccg
    40801 tttgataacg tcgacgacga gcaaattcaa aagccgcagc gttggaatcc ggcggatctg
    40861 gggcgcttta tgatcttctt cggaccgatc agctcgatct tcgatattct gacgttttgc
    40921 ctgatgtggt gggtattcca tgccaacacg ccggaaacgc aaacgctgtt ccagtcggga
    40981 tggtttgtgg tgggcttact gtcgcaaacg ctgattgtgc atatgatccg cacccgccgt
    41041 gtgccgttta ttcagagctg tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg
    41101 attgtcggga tcgcattgcc gttttcaccg ctggccagtt atctgcagct gcaggcgctg
    41161 ccgttaagct atttcccgtg gctggttgcg attctggcag ggtatatgac attaactcag
    41221 ttggtgaaag gattttatag ccgtcgttac ggctggcagt aaagaataaa ctgggcacga
    41281 tagccccgat tcagattgca gacaaagtaa gctttgttca tgccggatgc ggcgtaaatg
    41341 ccttatccgg ccaacaaatt caataaattg caggaatcac gtaggcctga taagcgtagc
    41401 gcatcaggcg attttgcgtt tgtcatcaga cttaatccgg gcatgatagc ccggatttcc
    41461 atcaagatta gcgacgaaca gcgatcgctt cgatctcaat cttcacgtct ttcggcagac
    41521 gggcaacttc aacgcaagaa cgtgccggga aggtggcgtt gtgttcggtg aagaaggctt
    41581 cgtaagtggc gtttacggtt gcgaagtcgt tcagatcttt cacaaacacg gtggttttaa
    41641 cgatgtcgcc cactttcagg ccagcggctt cgacgattgc ttttacgtta tccagcgact
    41701 gacgtgcctg tgctgcgacg tctgtcggta cttcgcccgt tttcgggttt accgggatct
    41761 gcccggaagt gatgatcatg ctgcccagat caacgccctg aacgtaagga ccgatagctg
    41821 ccggtgcatt ttccgtcgcg atagttttgc tcatgatttc tcctttatta cagcggtaaa
    41881 aagtctggct cattataggg agccagactt ttattaccaa ccgcaattaa ttggccagca
    41941 ccacattatg ggaaaactct ttttcacagt atttgcattt gagcgcgatg tcattggcgc
    42001 gttttcgcac ggcaaagctg gaagaaaccg gttcggcatg gctgatacag ttgctgttcg
    42061 ggcagaccag cacattgtcg atgcgctctg gcagactggg gcgcgattta cccaccactt
    42121 catagttgtc gatacggtta accgtggctt gcggcgcata caatgccagt tgatctacct
    42181 gatcttcact caaaaaggta ttttcgattt tgatcagatc tttgcggccc atctcgccag
    42241 aaggcaagtt cagaccgatg gtgatgcgct gatccgtttc ggtcagcttg aacagactca
    42301 acagcttaaa accgatctgg gcggggatat ggtcaattac cgtgccgcgt ttaatagctt
    42361 caacctgcaa tttattatcg tgtgtcatct ctatttcccc ttacagtatc agatcgcgat
    42421 tcagaaccag tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc tgctggaagt
    42481 accaggcgtg tggcgtttta tcaacatccg tcgcaatctc atcaacacgc ggcagcggat
    42541 gcagcacttt catattggct ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg
    42601 ctttcacgtt ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca
    42661 ggatgtctac ttccgccatc acttcttcaa tagagctgtg cagactccat gcgatccctt
    42721 tttcatcgag catatccaga atgtattgcg gcattgccag cgcgtccggc gcgatgaagt
    42781 aaaaacggtt gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg gtgcggccat
    42841 atttcaggtc accaaccatt gcgacgtgga gattgtccag acgcccctgg gtttcctgaa
    42901 tagtgaataa gtccagcaag gtttgcgtcg gatgttggtt ggagccatca ccggcattca
    42961 gtaccggtac attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc tgcggatgac
    43021 gcatcactat cgcatcgacg taagtgctga taaccgaaat ggtatcggcc agcgtttcgc
    43081 cctttttacc cagtgatgta ttggcgctgt cggagaagcc caccacgctg gcccccaggc
    43141 ggtgcataga tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag aaacagctgg
    43201 caatgacttt gtgcttcaac agctctggtt gcgggtttgc tttcagtttc gccgctgtcg
    43261 ccagcaccag attaaggtca tcgcgactaa ggttgtttat ggaaatgata tgtttctgat
    43321 atagcggatt agccatcttt tatctcctga cgcctgggca aaaaaaagcc cctcgattga
    43381 ggggctggga atgggtgatc aacgggaaga aaaacggcag gccagcgtct tttttcagac
    43441 gcggtaagac aaaatgtcga acacactgaa ccatacatcc tcccggcaaa ttgtccggca
    43501 ttatactcat cgtcagaagc ggcgcaagca tttgatgcaa tattttgtca gcgcaaacgg
    43561 tttgtttgaa ttaaaagtca aggtatatgc atttttattt gatgtgattc tgcaggggaa
    43621 ctgtccttcg gtatcaataa ttgcagacat tccctgcttt ttcctttttc actttcacgc
    43681 aatcagatat gcattttatt ccattcatcc gacttatagg gcggagtgtg aaagcgaacg
    43741 gctaacacta ttgcttactg ctcagggatg cgcgctatca ctttaatttc aaaatcaaag
    43801 cctgccagcc atgtaacacc caccgccgtc cagtttggat aaggtggggc gctaaatatt
    43861 tcatttttca ccgtcatgat gtcttcaaat tggttttctg gatcggtatg gaagctcgta
    43921 acatcaatga tatcgtcaaa agtgcatccc gcagctgcca gggtcgcatg caaattatca
    43981 aatgccagtc tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct
    44041 acttgcccgg aaacaaacag caaatcgccg gaacgaatag ccgcagaata acgatgctca
    44101 gcatatagtg aatgtcggcc agcagggaaa acagcggttc tttctaccat ttggttatcc
    44161 tcaagattta cgacatgaac agaagatttc tctttaccgg gagccgcttt tagcggacga
    44221 cgtgagtaaa caaaacccag acatcatgga taatggctgg gcttaattga gcgtagtcgg
    44281 ttatgcgcca aacgcgccat caatggtatg catcgcgccg gtaacaaaac tggcttctgg
    44341 ccctgctaac catgcgacca taccagcgac ctcttccggt tgcccatgtc ttttgatagc
    44401 catcaaacta tgcaacatat cgcgcattgg cccgttggcg ggattagcgt cggtatcaat
    44461 tggccctggc tggacgacgt taatggtgat cccacgcggt ccaaaatcac gggccagccc
    44521 gcgcgccatg ccttgcaggg cagatttgct ggcggcataa gcagccatgc ctgcaacagg
    44581 catacgatcg ccattcacgg agccgatgat taagatgcgc ccgccttcgg gcatctgccg
    44641 ggcggcttca acagaggcat gataaggagc atgaatattg attttgaaaa ggcgatcaat
    44701 atcgtcggca tttaattcca gggcctcgcc aaagacgcca atacctgcat ttaccaccag
    44761 gatatccaat gcgccgctct tacgaacgac atcaatgaca gcgtctctgt cagcactatc
    44821 tgtgaatact gctgtcgctc cagtctcttg tgccaggcgt ttagcggcat ctttcgaccc
    44881 cgcataggtg aatcgtacat tggccccatc ggtgacgaaa cgacgtacga tagcggcacc
    44941 gataccacga ctgccaccga ggatgagaac tgtcttacct gtaaaagcgc ccataaggac
    45001 tccttgattt attatgtaac atgcattaca aaactgtttt aactttctgt caacaggttt
    45061 tgtaatggtc actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga
    45121 gcaggcaatc tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg ctgtcagtgt
    45181 tgctgaagtt actgattatc ttggtattaa ccccccgagc ctctacgcgg cttttggcag
    45241 taaagctggg ttatttagcc gtgtactcaa tgaatacgtc ggtacggaag ctattccgct
    45301 tgccgatatt cttcgtgatg atcgtccagt aggcgagtgc ctggttgagg tattaaaaga
    45361 agcggcgcgc agatatagcc aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat
    45421 tcatagtcat gatccactag cgcgtgatat tgccgttcaa tattatcacg ccgcagaaac
    45481 gaccatttat gactatatcg ccaggcggca tccacaaagc gcacaatgtg tgactgattt
    45541 tatgagtacc gtgatgtcag ggctttctgc gaaggcacgg gaggggcact caatcgaaca
    45601 actctgtgca acagctgcac tggcggggga agcgataaaa actcttctca aggagtgatg
    45661 ctggccttga tccgaaaggc gggaacgcgc ctgccgataa gttgtgataa gacaataatt
    45721 cacgcgttaa ggctagcgga attgatcatc ttttcgtata acgatagaaa tgaaacgttg
    45781 ttttaattaa ggagtggaaa agatgatcat cggaaatatt cataatcttc agccgtggct
    45841 accccaggag ttacgccagg cgattgagca tatcaaagca cacgttacgg cagaaacgcc
    45901 aaagggcaag cacgatatcg aaggcaatcg tctgttttat cttatctcgg aagatatgac
    45961 cgagccgtac gaagcccgcc gtgcggagta ccatgcccgc tatctcgaca ttcagattgt
    46021 gttaaaaggt caggaaggca tgaccttcag cacgcaacct gcaggcacgc cggataccga
    46081 ctggttagct gataaagaca tcgcattttt gccggaaggc gttgatgaga aaacagttat
    46141 ccttaatgaa ggtgattttg ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc
    46201 agtgggtgca ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact
    46261 tttcgccgta aataactcca ggtttacggc gagtttgtga aaagagcgtt ttttgatatt
    46321 tttttgtga
//
LOCUS       DAZFAH010000015        45666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFAH010000015 DAZFAH010000000
VERSION     DAZFAH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.015"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(215..1219)
                     /gene="trpS"
                     /locus_tag="ACHJWB_000795"
     CDS             complement(215..1219)
                     /gene="trpS"
                     /locus_tag="ACHJWB_000795"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4312475.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1212..1970)
                     /gene="gph"
                     /locus_tag="ACHJWB_000796"
     CDS             complement(1212..1970)
                     /gene="gph"
                     /locus_tag="ACHJWB_000796"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4312476.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1963..2640)
                     /gene="rpe"
                     /locus_tag="ACHJWB_000797"
     CDS             complement(1963..2640)
                     /gene="rpe"
                     /locus_tag="ACHJWB_000797"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4312477.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2658..3494)
                     /gene="dam"
                     /locus_tag="ACHJWB_000798"
     CDS             complement(2658..3494)
                     /gene="dam"
                     /locus_tag="ACHJWB_000798"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4312478.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3601..4887)
                     /gene="damX"
                     /locus_tag="ACHJWB_000799"
     CDS             complement(3601..4887)
                     /gene="damX"
                     /locus_tag="ACHJWB_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4312479.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4979..6067)
                     /gene="aroB"
                     /locus_tag="ACHJWB_000800"
     CDS             complement(4979..6067)
                     /gene="aroB"
                     /locus_tag="ACHJWB_000800"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4312480.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6124..6645)
                     /gene="aroK"
                     /locus_tag="ACHJWB_000801"
     CDS             complement(6124..6645)
                     /gene="aroK"
                     /locus_tag="ACHJWB_000801"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4312481.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7046..8284)
                     /gene="hofQ"
                     /locus_tag="ACHJWB_000802"
     CDS             complement(7046..8284)
                     /gene="hofQ"
                     /locus_tag="ACHJWB_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4312482.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSDQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8196..8600)
                     /gene="hofP"
                     /locus_tag="ACHJWB_000803"
     CDS             complement(8196..8600)
                     /gene="hofP"
                     /locus_tag="ACHJWB_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4312483.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8590..9030)
                     /gene="hofO"
                     /locus_tag="ACHJWB_000804"
     CDS             complement(8590..9030)
                     /gene="hofO"
                     /locus_tag="ACHJWB_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4312484.1"
                     /translation="MNMFFDWWFAISPRLRQFCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9014..9553)
                     /gene="hofN"
                     /locus_tag="ACHJWB_000805"
     CDS             complement(9014..9553)
                     /gene="hofN"
                     /locus_tag="ACHJWB_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4312485.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9553..10332)
                     /gene="hofM"
                     /locus_tag="ACHJWB_000806"
     CDS             complement(9553..10332)
                     /gene="hofM"
                     /locus_tag="ACHJWB_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4312486.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10452..13004
                     /gene="mrcA"
                     /locus_tag="ACHJWB_000807"
     CDS             10452..13004
                     /gene="mrcA"
                     /locus_tag="ACHJWB_000807"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4312487.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13169..13729)
                     /gene="nudE"
                     /locus_tag="ACHJWB_000808"
     CDS             complement(13169..13729)
                     /gene="nudE"
                     /locus_tag="ACHJWB_000808"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4312488.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14049..16184
                     /gene="igaA"
                     /locus_tag="ACHJWB_000809"
     CDS             14049..16184
                     /gene="igaA"
                     /locus_tag="ACHJWB_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4312489.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            16249..16917
                     /gene="yrfG"
                     /locus_tag="ACHJWB_000810"
     CDS             16249..16917
                     /gene="yrfG"
                     /locus_tag="ACHJWB_000810"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4312490.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16928..17329
                     /gene="hslR"
                     /locus_tag="ACHJWB_000811"
     CDS             16928..17329
                     /gene="hslR"
                     /locus_tag="ACHJWB_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4312491.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17354..18232
                     /gene="hslO"
                     /locus_tag="ACHJWB_000812"
     CDS             17354..18232
                     /gene="hslO"
                     /locus_tag="ACHJWB_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4312492.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18367..20091)
                     /gene="yhgE"
                     /locus_tag="ACHJWB_000813"
     CDS             complement(18367..20091)
                     /gene="yhgE"
                     /locus_tag="ACHJWB_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4312493.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMVIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQRGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            20470..22092
                     /gene="pckA"
                     /locus_tag="ACHJWB_000814"
     CDS             20470..22092
                     /gene="pckA"
                     /locus_tag="ACHJWB_000814"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4312494.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22168..23520)
                     /gene="envZ"
                     /locus_tag="ACHJWB_000815"
     CDS             complement(22168..23520)
                     /gene="envZ"
                     /locus_tag="ACHJWB_000815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4312495.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23517..24236)
                     /gene="ompR"
                     /locus_tag="ACHJWB_000816"
     CDS             complement(23517..24236)
                     /gene="ompR"
                     /locus_tag="ACHJWB_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4312496.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24464..24940
                     /gene="greB"
                     /locus_tag="ACHJWB_000817"
     CDS             24464..24940
                     /gene="greB"
                     /locus_tag="ACHJWB_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4312497.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            25037..27358
                     /gene="yhgF"
                     /locus_tag="ACHJWB_000818"
     CDS             25037..27358
                     /gene="yhgF"
                     /locus_tag="ACHJWB_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4312498.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVKDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27796..28023
                     /gene="feoA"
                     /locus_tag="ACHJWB_000819"
     CDS             27796..28023
                     /gene="feoA"
                     /locus_tag="ACHJWB_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4312499.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28040..30361
                     /gene="feoB"
                     /locus_tag="ACHJWB_000820"
     CDS             28040..30361
                     /gene="feoB"
                     /locus_tag="ACHJWB_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4312500.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30361..30597
                     /gene="feoC"
                     /locus_tag="ACHJWB_000821"
     CDS             30361..30597
                     /gene="feoC"
                     /locus_tag="ACHJWB_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4312501.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30800..31678
                     /gene="rpnA"
                     /locus_tag="ACHJWB_000822"
     CDS             30800..31678
                     /gene="rpnA"
                     /locus_tag="ACHJWB_000822"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4312502.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(31707..32477)
                     /gene="bioH"
                     /locus_tag="ACHJWB_000823"
     CDS             complement(31707..32477)
                     /gene="bioH"
                     /locus_tag="ACHJWB_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4312503.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            32515..33198
                     /gene="gntX"
                     /locus_tag="ACHJWB_000824"
     CDS             32515..33198
                     /gene="gntX"
                     /locus_tag="ACHJWB_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4312504.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            33257..33832
                     /gene="nfuA"
                     /locus_tag="ACHJWB_000825"
     CDS             33257..33832
                     /gene="nfuA"
                     /locus_tag="ACHJWB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4312505.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34193..35509
                     /gene="gntT"
                     /locus_tag="ACHJWB_000826"
     CDS             34193..35509
                     /gene="gntT"
                     /locus_tag="ACHJWB_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4312506.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(35554..37638)
                     /gene="malQ"
                     /locus_tag="ACHJWB_000827"
     CDS             complement(35554..37638)
                     /gene="malQ"
                     /locus_tag="ACHJWB_000827"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4312507.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(37648..40041)
                     /gene="malP"
                     /locus_tag="ACHJWB_000828"
     CDS             complement(37648..40041)
                     /gene="malP"
                     /locus_tag="ACHJWB_000828"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4312508.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            40653..43358
                     /gene="malT"
                     /locus_tag="ACHJWB_000829"
     CDS             40653..43358
                     /gene="malT"
                     /locus_tag="ACHJWB_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4312509.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(43401..44417)
                     /gene="rtcA"
                     /locus_tag="ACHJWB_000830"
     CDS             complement(43401..44417)
                     /gene="rtcA"
                     /locus_tag="ACHJWB_000830"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4312510.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(44421..45647)
                     /gene="rtcB"
                     /locus_tag="ACHJWB_000831"
     CDS             complement(44421..45647)
                     /gene="rtcB"
                     /locus_tag="ACHJWB_000831"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4312511.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTTLQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        10821 a        12496 c        11584 g        10765 t
ORIGIN      
        1 gcaactgtga gatgtggaaa cggcgaggcg cttcacattt tttcggatta ttgcaaatga
       61 ggataaaaaa accgggtttc cccggtttca gagtgatgac gaagtgattt attcgacaat
      121 gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc
      181 aaaggggcga ggggaccgtc cgagcgtttt gatattaagg cttcgccaca aaaccaatcg
      241 cttcgtacac cgctttcagc gtacgggaag cgtgtacact ggctttttct gcgccgtctt
      301 tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt
      361 cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc
      421 cttcgaactg tttttccagt tctgggatgc tctggcccgt taccgcagaa aggatatcca
      481 gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg
      541 agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga
      601 taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca
      661 ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca
      721 gcgcgttgaa acgctgggcg atatcgcgac tcagttccag gtgctgtttc tggtcttcac
      781 ccaccggtac cagattagtt tgatacagca ggatgtccgc agccatcagc accggatagt
      841 caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgggtca
      901 tgcggctcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt
      961 ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat
     1021 acaaggccag cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga
     1081 tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact
     1141 gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa
     1201 aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg
     1261 ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg
     1321 ccgtagttat atccgtaggt taagccaact gatgggcaac ctgccgcttt tgccgcctga
     1381 atatcattgc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca
     1441 gccaccagta acagcgggtc cggatgcggt tttttgtttt gcacatcatc gccgccgata
     1501 accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc
     1561 ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg
     1621 gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgctatagta gcgatcgaac
     1681 agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggttta
     1741 cccattgttt tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc
     1801 agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc gatgggcaac
     1861 tccagcgcat acagcgccat atctaccgca gcagcaagac cgggagcact gtcgaccagc
     1921 gtaccatcaa gatcaaaagc gacgccgcga atatcttcaa acttattcat gacttacctt
     1981 tgccagttca ctgcgcattt catcaatgac ttttttgtag tctggctggt cgaagattgc
     2041 cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt
     2101 cacgccaccg tccacttcca gacgaatgtc aaagccagac tcgtcgatac ggcgacgtac
     2161 ttcgcgcagt ttatccagtg tttgaggaat gaaagactga ccgccgaaac caggattgac
     2221 ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagatagc tcagaggtgt
     2281 cgccgggtta aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg
     2341 gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa
     2401 atcaggcaca atgcgatcga cgggtttcac catcagatgt acgtcgatag gggcggtaat
     2461 gccatagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg
     2521 gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc
     2581 ttcacctagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat
     2641 ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc
     2701 agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg
     2761 acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag
     2821 atcagcactg gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt
     2881 tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt
     2941 gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgcgccat gctatcggcg
     3001 taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct
     3061 tccgggaaat agggtttttt gtagcggcca aacggcacat taaactcacc gcgcagattg
     3121 taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg
     3181 aacggatcct ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt
     3241 gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca
     3301 atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg
     3361 gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccaaacattc gcccttgggc
     3421 aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg
     3481 cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga
     3541 cagcatccag cgcttcaggc aactcccgca acgataaacc attgcgggag ataaccttga
     3601 ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctggac
     3661 atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga
     3721 aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag
     3781 attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag
     3841 agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc
     3901 agcggctggt gttgccgtgg tttgcgccgg ggatgccgtt tgtactggag ccgtagtcgc
     3961 cgtctctttt ggtgctggcg tagaagtcgc agccggtgcc ttcgtgctcg ccacaggagc
     4021 agctggttca gtacgcttcg gcgtctgtgc taccggcttc ggctccgttt tcacggttgc
     4081 ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct ggacgcgtag tggccggacg
     4141 ttcagcagtt tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgta ctggcgcgac
     4201 agttgctggt tcagtcggca atgtagaatt gaccgccaca ttgttcaact gttgctgatt
     4261 ttgtggttgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc
     4321 cgttgccgcc ggggtttgcc cttgagtcgg cgtagaagag atcggcggca gagaaacatc
     4381 ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat tggcctgatc
     4441 ggtcgcattg ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaaga
     4501 ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat
     4561 gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt
     4621 acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc
     4681 ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc
     4741 agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc
     4801 agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag
     4861 ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac
     4921 gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt
     4981 acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa
     5041 cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct
     5101 tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg
     5161 gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc
     5221 cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta
     5281 accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca
     5341 gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac
     5401 aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat
     5461 tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg
     5521 ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca
     5581 ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag
     5641 ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac
     5701 gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc
     5761 caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg
     5821 tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt
     5881 taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt
     5941 cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat
     6001 taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc
     6061 tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca
     6121 gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc
     6181 atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa
     6241 cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg
     6301 ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc
     6361 ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat
     6421 accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc
     6481 gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc
     6541 ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc
     6601 ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact
     6661 aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga
     6721 aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg
     6781 gcaactgaat aaatcaccca tccccctgca tatctggtcg ctgcaagcgc gctgccttgc
     6841 taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc
     6901 cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat
     6961 cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcgta
     7021 aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca
     7081 ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg
     7141 gaatgtcgcc aagcaacggc acgctatcct gatccgattt atttttacgg gtaaaaatgc
     7201 cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcaatc tcctgcttat
     7261 caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga
     7321 tgtgtaattt cagccgaatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca
     7381 ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg
     7441 gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac
     7501 tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaaac
     7561 gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt
     7621 cgctgccaag cgtggtgact tgcccaacgc caccagcttg ttgcgcatcg gccagcgtcc
     7681 atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct
     7741 caacctgccc gaccggaaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg
     7801 ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg
     7861 cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta
     7921 tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg
     7981 cgatattgtt attctgccag gcaatggaat gcactgagag aatgttgtct tcctgccgcg
     8041 ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta
     8101 gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt
     8161 cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt
     8221 gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct
     8281 tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggtt
     8341 cccagcgtta gtgcgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg
     8401 ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgtgctcg
     8461 cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct
     8521 tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac
     8581 aaccagcgtt taaccctcat gcggtgtctc cagttgtagc gtgaacaaaa gatcatcacc
     8641 ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc
     8701 cgacggcact gcttcccaca gcgttttcaa cgccaactca cctccctgcg cggatggatg
     8761 ccaggaaacc agttgcgcgc cggataactg aaaatccaga ggcgaaaagg gcagcgtttt
     8821 ttcctcgctg aagggagtgg tgtctaccag gcgatacagt gtggcccact gctggtgatg
     8881 acttgcccgt agtcgaatta atgcgtcgct ctcttcatgg tgtgtcgatg acagaaaaat
     8941 gagcgtaact aacatcagca accagactgc ccagcaaaac tggcggaggc ggggtgatat
     9001 ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa
     9061 attgccagcg tccctgcgca tcctgctgcg tggccccccg ctgattgaga tgaaaagaag
     9121 catcctggcg aagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtaagcccct
     9181 tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcgtgt tcaggtaaaa
     9241 gagccgccag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc
     9301 tttgcaaacg ctgctcacga atttgttggc gctccagcaa acgtggtttc gttatctgta
     9361 agctgtgggc gagttgttgt tccccctgaa gcaaaacggc gtctacgcga gcttcggcgc
     9421 tgcttgtcag acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca
     9481 tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa
     9541 ttggcaggtt catcagtacg ccttcccaag cgccagcccc agcgcgatgg caaagtctcc
     9601 acctggcggc ggtagcggcg gctgacgaac agaaacggcc tcccagggat caaatccgcc
     9661 ttcgccacat atcgcgacgc tgtcgggatc aacggataac gctgccgcca gctccttcgc
     9721 gctagtcatc cctaccgcca gtttgcgccc ccagcgatag cgtgtcgccc acagccactg
     9781 ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaattgctg
     9841 taatgcactg gcatccgggg tgatcgcgct cacatgaaca cgcaaccttt ctgccagagt
     9901 aagtagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataggcgg ggctgagtga
     9961 gtcttcgctg taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt
    10021 ccctgacagc caggccgttt gctcccgctc accaagggac atcgacgggc gcggaaatga
    10081 ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc
    10141 gcgactccac ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc gcccatcttt
    10201 gataatgtcg ttctccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgctcc
    10261 ccgcacgatc gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat
    10321 cttaaatgcc attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg
    10381 tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg
    10441 ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc
    10501 tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg
    10561 cgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc
    10621 tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccgccgg
    10681 agatggtgaa agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg
    10741 acccggtggg gatcttccgt gcggcaagtg tggcgctgtt ctccggccac gcgtcacaag
    10801 gggcgagtac cattacccag cagctggcga gaaacttctt cctcagtcca gaacgcacgc
    10861 tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag
    10921 acgagatcct cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg
    10981 gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca actgacgctg aacgaaatgg
    11041 ctgtgatagc cgggctgccg aaagcgcctt ccacctttaa tccgctctac tcaatggatc
    11101 gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct ggatgaaggg tatatcaccc
    11161 aacaacagtt cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga
    11221 ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg
    11281 gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc
    11341 aggccgcaca gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc
    11401 gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac aacaagatta
    11461 ccgatacgct gaaggcgctg ccaacctatg gtccgctgct gcctgccgca gtcaccagcg
    11521 ccaatcctca gcaagcgacg gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg
    11581 aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca gcaaggaccg acgccgcgta
    11641 aagtgaccga tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat
    11701 ggtggctggc acaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg
    11761 gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca
    11821 cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg
    11881 ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttcccgc tgggatgcag
    11941 gtgccggttc tgactggcag ccgaaaaact cgccgccaca atatgccggt ccaattcgct
    12001 tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg
    12061 gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcgcaa aacattgtcc
    12121 acaccgaatc gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct
    12181 acgcggtcat ggcgaacggc ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa
    12241 acgatcaggg tggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata
    12301 ttccagtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag
    12361 atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg
    12421 caaatcaggc gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca
    12481 ctccgctggc attcctgatt aagagtgctt tgaacaccaa tatctttggt gagccaggct
    12541 ggcagggtac tggctggcgt gcaggtcgtg atttacagcg tcgcgatatc ggcgggaaaa
    12601 ccggtaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga
    12661 cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg
    12721 gagcgattaa agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat
    12781 gggacgctta tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgcctc
    12841 cgggtattgt gacggtgaat atcgatcgca gcaccgggca gttagctaat ggtggcaaca
    12901 gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaggcggtg cacgaggtgg
    12961 gaacgaccat tatcgataat ggtgaggcac aggaattgtt ctgattaaaa aggcgcttcg
    13021 gcgccttttc agttggctga caaaatgcat ttgtttatgc cggatgcggg tgaacgcgtt
    13081 atccggctaa caaaatcgtg aaaaatcaat atattgcagg aaccccataa gcctgataag
    13141 cgttgtgcat caggcaaact tcacgcattt acactcgccc ctgccctttc aaccattcgc
    13201 gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa gtcagggtct tccagcaaat
    13261 ccatcatatg cgccagcggc cagcgcacct gtggtagcgg ctctggctca tcgccttcca
    13321 gcgattccgg gtagagatct tgcgcgacca cgatattcat tttgctggaa aagtaagacg
    13381 gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc accaaatcca acctcttctt
    13441 ttagctcgcg gttagcggct tcgtagacgc tttcacccgg atcaattaat cctttcgaaa
    13501 aacctaattc gtaggattca gttcccactg cgtattcgcg gatcaggatc aggtgatcgt
    13561 ccacaatcgg cacaatcatc actgcttccc ggttggttgg acgcattcgt tcataaacac
    13621 gccgcacgcc attgctgaac tccagatcca cgctctcgac ggtaaacagt cgggaacggg
    13681 ctacagtttc aacattcaga atggtgggtt tttgtaatga tttgctcatc gtgggatcta
    13741 tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc tttcggcaat gtgaattgca
    13801 tgttatttac atttatgtaa cttaataaat aatcgtcctc aaatcaaatt aaaagtcaat
    13861 aggttgaaat aactccagga atttgctgat attccgcctt cagagggttt gctatgatca
    13921 gcggttactg tgatgtgctt aatgatgctc aagttaaact ccacgcttgc cgatagccaa
    13981 ccgcagaatc atgtattgtg tctggtgcga ctgaccacgc ctgacagact aagtaagatg
    14041 gggaaagcat gagcaccatt gtgatttttt tagctgcttt gctggcctgc tcactacttg
    14101 cgggatggct gataaaagtg cgatccagac ggcgtcagct gccctggacc aacgccttcg
    14161 cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag cgccgttgaa aattatcttg
    14221 agagcctgac gcaggtatta caggtgcctg gcccaacggg agctagcgcg gcaccgatct
    14281 ctctggcgct gaatgccgaa agcaacaacg tcatgatgct gacacacgct atcacacgtt
    14341 acggcatctc taccgacgat ccgaataaat ggcgttacta cctcgattcg gtagaagtcc
    14401 acctgccccc cttctgggaa cagtacatca acgatgagaa taccgttgaa ctgattcata
    14461 ccgattcgct gccgttggtt atttcactca acggtcatac gctgcaggag tacatgcagg
    14521 aaacgcgcgg ctatgccttg caacctgttc cgtccacgca ggcgtcgatt cgcggggaag
    14581 agagtgagca aatcgagcta ctcaatattc gcaaagaaac gcatgaagaa tatgcgctga
    14641 gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc ctccttcctg atgttctttt
    14701 tctgcctgat taccccggat gtatttgttc cgtggctggc aggcggcgca ttactgctgc
    14761 tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg cgggaaattc
    14821 attgtctgcg cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat caggaacaga
    14881 tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg cagccataca
    14941 ttgctcagga tctcggtcaa caaaccgata ttgatatcta tctcgaccgc cacgtagtgc
    15001 gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta cagcactggc
    15061 tgcgcagtac gattatcgct gcgggctcgc tgctggtgct gtttatgctg ttattctgga
    15121 tcccgctgga tatgccgctg aaattcactc tctcatggat gaaaggcgca cagaccattg
    15181 aagccaccag cgtaaaacaa ctggctgacg ctggcgtccg ggtaggcgat acattgcgta
    15241 tcagcggtac gggaatgtgt aatattcgca cttccggaac ctggagcgcg aaaaccaatt
    15301 cacctttttt accgtttgac tgctcgcaga tcatctggaa tgacgcccgc tcattgccat
    15361 taccagaatc tgaactggtc aacaaagcga cggcattgac tgaagcagtt aatcgccagc
    15421 tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attacgttca gcgattcaaa
    15481 aatccggcat ggtattgctt gatgattttg gcgacattgt actgaagaca gcggatttat
    15541 gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc ggcaacagta
    15601 aagactggga cgcgctggta aaacgcgcca acgccgggaa gctcgatggc gtgaatgtgt
    15661 tattacgccc ggtgagtgcg gaatcgctgg ataacctggt ggcaacctcc accgcgccgt
    15721 ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc cccggcggat
    15781 tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct tcggcatcac
    15841 tttacgacta tccgccgcaa gaacagtgga acgctttcca gaaactggca caaatgctga
    15901 tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac gccaatggta
    15961 cgcagcatat tggccttcat ccaatcccgg atcgttccgg cctgtggcgc tatctcagca
    16021 ccacattgct gctactgacg atgctgggta gcgccattta caatggcgta caggcctggc
    16081 gtcgttacca gcgtcatcgc actcgcatga tgaagattca ggcctattat gaaagctgcc
    16141 tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt tgcgcggcag
    16201 gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat gcatatcaac
    16261 attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacgtt gctcgacctc
    16321 gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc gaaaaacggg
    16381 gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt acagcatacg
    16441 ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat ctgtgcgatg
    16501 accaccgaga tgggaccgcg cgccgtactg cgtgaagata ccattccgtt tcttgaggca
    16561 ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg cgcatccgca caacctggcg
    16621 gtaaaacttg agcataccgg tctggacgca caccttgatt tattactttc cacccacaca
    16681 tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc tacgggtctg
    16741 aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc tgccgcgcaa
    16801 tttggtattc gttactgcct cggcgtgact aatcctgatt ccgggattgc cgagaaacag
    16861 tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct aatgtgaagg
    16921 agacgccatg aaagaaaaac ctgctgttga ggttcgactg gataaatggc tatgggctgc
    16981 ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta aggtgcatta
    17041 caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc tcactctgcg
    17101 ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc gtcgccccgc
    17161 cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac gcgaaaaaat
    17221 ggcgatggca cgtaaactta atgccttaac catgccgcac ccggaccgac gcccggacaa
    17281 aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac tgtcacctgc
    17341 aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg aaaactttgc
    17401 cgtgcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg agaaccacga
    17461 ttatccgcag cccgttaaaa acgtgctggc agaactgctg gttgcgacca gcctgttaac
    17521 cgctacgctg aagtttgatg gtgatatcac cgtacagctg cagggcgacg gtccgatgaa
    17581 tctggcggtt attaacggta acaataacca gcaaatgcgc ggtgtggcgc gcgtgcaggg
    17641 cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg tggtgatcac
    17701 cattaccccg agcgaaggcg aacgctatca gggcgtagtt ggtctggaag gtgataccct
    17761 ggcggcctgc ctggaagatt actttatgcg ttctgaacag ctgccgacgc gcctgtttat
    17821 tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc aggtaatgcc
    17881 tgcgcaaaat gcccagcagg acgactttga ccacctggca acgctaaccg aaaccatcaa
    17941 aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt atcacgaaga
    18001 agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct cgcgtgaacg
    18061 ttgcgccgat gcactgaaaa cgctgcctga tgaagaagtt gatagcatcc tggcggaaga
    18121 tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca atgcgatgga
    18181 tattgctgaa atccgcaaca acgcgtctcc ggcagatccg caagttcatt aatgagtatg
    18241 tccggcagag agggctctgc cggattttac aacttaatgg cctgatgtgc tgcgcttacc
    18301 agcgcctatg aaataaatcg caaattattt tggtcagata cagcgtcgta tccgataaaa
    18361 gcgttgtcac ttacttaagt ttatttccag cgtttcacca tctaccgtca gatcgcgcca
    18421 cgctttcggc ctcgtcccca gttttccatc cttcgcagct gtgagtaatt tttctttcgt
    18481 tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt ctgagtcaaa
    18541 gccatagaca atgactctgt catcattaaa agcaaacagg atccgctccg cctgaccatc
    18601 actattcaga tcttgctcga ccagtacgca ggcgtctttt tcaatacagg tcatcacgtt
    18661 atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag aaccaggggc
    18721 aattaacaca ttttcggcta atactttttc cgatacctgt tcctgaagat gttgctctcc
    18781 atctaacgcc atcagcagat cccgcgcgcg tttcgggtct ttcataaacc cggcatcgct
    18841 tttcagcgac tcaagcgccg cacgtccata gcgacctctc tgttcgagca tgtagatagt
    18901 tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt tcacgctaat
    18961 gcgcatactg tccagcaccg gcgaattaag aagcaccagt atcaccaaca ccaataacga
    19021 aaccgcaagg ttcactttgc cctgaagaac aaggggattt tgcccattac gccagacgat
    19081 actgacaaaa taccccagcg accacaccag taacaccagc accgccagcg cgccctgcaa
    19141 gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacgcccagg cggcgacgaa
    19201 cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg gccccgtcca
    19261 gggaagtgac gctttttgcg gatcgcggac aataaccatt aagatcaatt gcaaaaaggc
    19321 cagcgttaac agcaacccgg cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa
    19381 cggcagggtg atgataaaca tcagggttag caatgatact aacggcagca accccgtggc
    19441 gattagcgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac gcgcgaggat
    19501 caccgccagt gcggtaacca gacctaatgt aagatacata aaccagtcgg ttgcaaaaaa
    19561 gagcgtattg aaaaacgtga tgccaacaag tttaaacaac tcaccccaca gtaaaagcac
    19621 cagccacgtc aggccattgg cgagaaaaat caccagtaat ataagtacgt tatgccatac
    19681 tgactggtaa aaatagcgat agcggctact gtcattgcgg atgcgaaggc tttgttgtat
    19741 ccacggtagc aacaacattg ccatcagcag cagatagcaa ccaaaatccc aaagcgcctt
    19801 ttcagctctc caggggttca tgccatcagt ttgccacttc aaccagccgc tcatccccag
    19861 cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac
    19921 ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa caatccaact
    19981 gtgatttttt ccggcaagcc aggcgataag caggtagcaa accagcccct gaagtaatcc
    20041 ggtagcaatc atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat
    20101 ccagaatcaa aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga
    20161 aaataatcaa aaaaagttag cgtggtgaat cgatacttta ccggttgaat ttgcatcaat
    20221 ttcattcagg aatgcgattc cactcacaat attcccgcca tataaaccag gatttaacct
    20281 tttgagaaca ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga
    20341 caggaatcac ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc
    20401 ttgtcgcggt taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg
    20461 agcagtgaaa tgcgcgttaa caatggtttg accccgcaag aactcgaggc ttatggtatc
    20521 agtgacgtac atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc
    20581 gatccgagcc tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc
    20641 gataccggga tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc
    20701 actcgcgata ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc
    20761 tctccggaaa cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt
    20821 ctgttcgttg tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc
    20881 atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat
    20941 gaagagctgg caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac
    21001 ccgcagtgga aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag
    21061 cgcatgcagc tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg
    21121 atgatgaact acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt
    21181 ggcgagaaag gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt
    21241 tccaccgacc cgaaacgccg cctgattggc gatgacgaac acggctggga cgatgacggc
    21301 gtgtttaatt tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa
    21361 cctgaaatct acaacgctat ccgtcgcgat gcgctgctgg aaaacgtcac cgtgcgtgaa
    21421 gatggcacta tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatccg
    21481 atctatcaca tcgataacat tgttaagccg gtttccaaag cgggccacgc gactaaggtt
    21541 atcttcctga ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat
    21601 caaacccagt atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc
    21661 atcaccgaac cgacgccaac cttctccgct tgcttcggcg cggcgttcct gtcgctgcac
    21721 ccgactcagt acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat
    21781 ctggttaaca ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgcc
    21841 attatcgacg ccatcctcaa cggttcgctg gataatgcag aaaccttcac tctgccgatg
    21901 tttaacctgg cgatcccaac cgaactgccg ggcgtagaca cgaagattct cgatccgcgt
    21961 aacacctacg cttctccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt
    22021 atcgacaact tcgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt
    22081 ccgaaactgt aatgatttga agctggagaa tatctatcca gtatcttata gaaagcaaaa
    22141 cgggaggcac cttcgcctcc cgtttattta cccttctttt gtcgtgccct gcgcccgcgt
    22201 taccggcact ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc
    22261 cagcatcccg ttatggttat ccacgatacg ctgcacaatt gccagcccta atcccgtgcc
    22321 gctaatggtg cgcgcactgt cgccgcggac aaacggctgg aacaggtgct tacgttgttc
    22381 cggcgcaatt cccggaccgt catcttccac ctggaaccag gcgcgattcg gctccgttcc
    22441 gctgctgact ttgatccagc cattgccata acgggcggcg ttgaccacca tattcgccac
    22501 cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt
    22561 ttcaatttcc cgctcatagc cactttcggc agcaatcacc tcaccgagta ctgcattaag
    22621 atccgccatt tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat
    22681 gatggcgttg cactcttcga tatctttatt gatcgattct gccagatagc catcctgctc
    22741 gctcatcatc tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt cgtgacttac
    22801 ccccgccatc agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatggtt
    22861 aaaggcacgg gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat
    22921 aatcccttta ccaacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat
    22981 acgaataaac agccacgccc cgcctatcgc caatagcata atcgccagcg tatagcggaa
    23041 cagcggagag aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc agatattggg
    23101 cgacagccag gttttcagcc agacgacagg cgaacttttg ttgacctcaa cgcgcacttc
    23161 cgtcgggccg cccagttgct gtgccatctg atggcttaag aattcatagt gttgcgccca
    23221 acgcagacct gcctcttcgg cagcctcgtt ggagtagaga gagatcccca gctcacggta
    23281 gatctcccga cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt
    23341 gtcggtcatc aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg
    23401 caaaatcgcg aagttcagca ccaccagata agtcgtcacc aggctggcga acagcaaggt
    23461 gacgatgagc aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat
    23521 gctttagagc cgtccggtac aaagacgtag cccaggcccc aaacggtctg aatgtaacgc
    23581 ggatgcgctg gatcttcttc taccatgcgg cgcagacgcg aaatctgcac gtcgatggag
    23641 cgttccattg cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc
    23701 ggctcacgcg gatggctgac cagtgccttc agtaccgcaa actcaccgct ggtgagcggc
    23761 atcggctcat cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa
    23821 gcaattaccg cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc
    23881 accgcacgaa tacgggccag cagttcacgc gggttaaacg gttttggaat gtagtcgtca
    23941 gcgccaatct ccaggcctac gatacggtcc acttcttccc ctttcgccgt caccataatg
    24001 atcggcatcg ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc atcttcacca
    24061 ggtaacatta aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc
    24121 tgttctgcat tagcgacgct tcgaacctgg aagccttgtt cggtgagata acgttccagc
    24181 agcgcacgca ggcgcatgtc gtcatcgacc accagaatct tgtagttctc ttgcattgtt
    24241 tgtactccca aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg
    24301 tcaccagcta aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca
    24361 gcttaagtat acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat
    24421 acccaatgtg cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt
    24481 tacccgggaa gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg
    24541 cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc
    24601 tgactatcag tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac
    24661 taaatgcatg gaaaatctca aaatcgtcga ttactcccct cagcaggaag gcaaagtctt
    24721 ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt
    24781 cggctacgat gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg
    24841 cgcattgctg aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc
    24901 gagctggtat gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg
    24961 ccgatggctg gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa
    25021 aagatgaact caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca
    25081 ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc
    25141 gtttatcgca cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa
    25201 tctggaaacg cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa
    25261 atccatttcc gagcaaggca aactcaccga tgatctggcg aaggccatca acgccaccct
    25321 aagcaaaacc gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgcgg
    25381 gcaaatcgcc attgaagcag ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc
    25441 acacacgcca gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg tggcagatac
    25501 caaagccgcg ctggatggcg cgcgctatat cctgatggaa cggtttgccg aagatgccgc
    25561 gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg catttggttt ctacggtggt
    25621 gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt
    25681 gtccacggtg ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact
    25741 ccagctttcg ctgaatgccg atccgcagtt cgatgagccg cccaaagaga gctattgcga
    25801 gcaaatcatc atggatcacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg
    25861 caaaggcgta gtgagctgga cctggcgcat caaggtgctg atgcatctgg aaaccgaact
    25921 gatgggtacc gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct
    25981 gcacgatctg ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg
    26041 tctgcgtacc ggggtaaaag tggcggtggt cgatgccact ggcaaactgg tggcgaccga
    26101 taccatttac ccgcacaccg gacaagccgc aaaagcagcg atgactgttg ctgcgctgtg
    26161 tgaaaagcat aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaaccga
    26221 acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga aagtgatcgt
    26281 cagcgaagct ggcgcgtcgg tttactcggc ttccgagctg gcagcgcagg agtttccgga
    26341 tctcgacgtt tcgctgcgtg gcgcggtgtc tatcgcccgc cgtttgcagg atccgctggc
    26401 ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag
    26461 ccagacgcaa ctggcccgca aactggacgc agtagtagaa gactgcgtaa acgccgttgg
    26521 cgtcgatctc aacactgctt ctgttccgct gttaacccgc gtggcgggcc tgacgcgcat
    26581 gatggcgcaa aacatcgtgg cctggcgcga tgagaacggt cagttccaga accgtcagca
    26641 actgctgaaa gtgagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcttgcg
    26701 cattaaccat ggtgataacc cgctggacgc gtctaccgtc cacccggaag cctatccggt
    26761 ggtggaacgc attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag
    26821 cgaactgcgt aacctgaaag cgtctgactt tactgatgaa aaattcggtg tgccgacagt
    26881 aactgacatc atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac
    26941 cgctcagttt gccgatggcg tcgagacaat gaacgacctg caaccgggta tgatcctcga
    27001 aggtgcggtg accaacgtca ccaactttgg cgcgtttgtc gatatcggcg tgcatcagga
    27061 cggcctggtt cacatctctt cattgtcgaa caagtttgta aaagatccgc ataccgtggt
    27121 gaaagcgggc gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat
    27181 cgccctgacc atgcgtctgg acgagcagcc tggcgaaacc aacgcccgtc gcggcggcgg
    27241 taatgaacgc ccgcaaaaca accgcccggc agccaaaccg cgcggacgtg aagcgcagcc
    27301 tgccggtaac agcgcgatga tggacgcgct ggcggcagca atgggcaaaa aacgttaaac
    27361 gccctgattg acccctaaac ctgcgcgata aggtgtccac gccgcatctg gcaacgaacg
    27421 ccttgtctga cataaaagtg ccggagaata tctccggcat ttttattcca cagccaaact
    27481 cacaatatat tccggcaaca tttatcactt cattaacaac tgaaacctta attaaacatt
    27541 agccagtcct ggttattcgc tattcgaatt atattttcgc tgcgatataa ccttgagcca
    27601 catcaacatt gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct
    27661 gatagaaacc attctcatta tcattatgct gttgattatt taatctctcc ttcgttggca
    27721 aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg tagttatcca gttaatgaga
    27781 aacaagtagg cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc
    27841 cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc taggcatgtt acctggctcc
    27901 tcttttaatg tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt
    27961 gtgagcctgg tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt
    28021 taatacggtg ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc
    28081 tggcaagaca accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc
    28141 tggcgttacc gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct
    28201 ggtggacctg cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga
    28261 gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga
    28321 tgcgtctaac cttgagcgta acctgtacct gacgctacaa ctgctggaac tcggcattcc
    28381 ctgcatcgtg gcactgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat
    28441 tgatgctctg tcggcgcgtc tgggctgccc agtgatcccg ctggtttcaa cccgtggtcg
    28501 cggtattgaa gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact
    28561 ggtgcattac gcacagccgc tactcaacga agcagattca ctggcaaaag tgatgccttc
    28621 cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta
    28681 cagccgcgcc tacgccggtg aagcgtcgca gcatctggat gccgccctcg cccgtctgcg
    28741 taatgagatg gacgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc
    28801 catctgtgat gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt
    28861 agataaaatc gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct
    28921 gatgttcctg ctggctatca acatcggcgg ggcgttacag ccgctgtttg acgtcggctc
    28981 cgtggcgcta tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg
    29041 gctgactatc ttcctcgccc agggcctggg tggcggcatt aacaccgtgc tgccactggt
    29101 gccgcagatt ggcatgatgt acctgttcct ctccttcctt gaggactccg ggtatatggc
    29161 gcgtgcggcg tttgtgatgg accgtctgat gcaggcgctg ggcttgccgg ggaaatcctt
    29221 tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggtg cacgtacgct
    29281 tgatgcaccg cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc
    29341 gcgtctggct atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgcgctggc
    29401 ggtcttctcg ctgtatatgc tgggtattgt gatggcggtg ctgactggcc tgatgctcaa
    29461 gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg gagttgccgg tctatcatgt
    29521 accacacgtt aaaagcctga ttatccagac ctggcagcgt ctgaaaggct tcgttctgcg
    29581 tgctggtaaa gtgatcatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgct
    29641 gagcgggaaa atcgtcgata acatcaacga ctcggcgctg gcgtccgtca gccgggtgat
    29701 caccccggtc ttcaagccaa ttggcgtgca tgaagataac tggcaggcaa cggttggcct
    29761 gtttacaggt gccatggcga aagaagtggt agtgggtacg ctcaacaccc tctacaccgc
    29821 agaaaatatt caggacgaag agttcaatcc ggcagagttt aacctcggtg aagagctgtt
    29881 cagtgcgata gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat
    29941 gaaccccatt gaagccagca aaggcgacgg cgaaatgggt accggggcga tgggcgtgat
    30001 ggatcagaaa ttcggtagcg cagcagcagc ttacagctac ctgattttcg tcctgctgta
    30061 tgtaccatgt atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg
    30121 cttctccatc ctgtgggggc tgaatatcgc ttactcgctg gcaacattgt tctatcaggt
    30181 cgccagctac agtcagcacc caacttacag cctggtgtgc attctggcgg ttatcctgtt
    30241 taacatcgtg gttatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct
    30301 ggcaacccgc aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta
    30361 atggcttcac ttattcaggt gcgcgatttg ctggcgttac ggggccgtat ggaagcggcc
    30421 cagataagcc agacattgaa cactccacag ccaatgatta acgccatgct gcaacaactg
    30481 gaaagtatgg gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt
    30541 tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt ggtgggcgct gcgttaacct
    30601 tactccatcg cctgttttgg aaagcccggt atgcgtctgc atccgggctt ttttgcgtgc
    30661 ggctttccat aaaaatgcaa ctcttgcagc acggcgtaag ttcctttgaa agcatctcgc
    30721 agggatgaaa actcgctaat acacaggtgt ggagtggcgc gtagagtcgc ggcattcaaa
    30781 caacaggtga aggaacgcca tgagcaaaaa gcagagttcc accccacacg atgcactgtt
    30841 caaactcttt ttacgccaac cggacacggc tcgtgatttt cttgcgtttc atttaccggc
    30901 acccattcac gcgctttgtg atatgaaaac cctcaagctg gagtcgagca gctttattga
    30961 tgacgatctg cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc
    31021 aggatacatc tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg
    31081 catgatgcgt tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt
    31141 gccgatggtg gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct
    31201 gtgttggctg gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt
    31261 tccgctgatt gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc
    31321 gctgctggag ttaattcaaa aacatattcg tcaacgcgat ctgatggggc tggtagagca
    31381 aatggcctgc ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa
    31441 ttacatactg caaaccggcg acgcggtacg ttttaacgat tttatcgacg gcgttgccga
    31501 acgttcaccg aaacacaagg agagtttaat gacaattgcg gaaagattgc ggcaggaggg
    31561 ggaacaatcc aaagccctgc atatagccaa aataatgctt gaatccggag tccctcttac
    31621 agacatcatg cgctttaccg gggtgtcaga agaagagttg gctgcggcga gccagtaaat
    31681 ttctgtctcg ccatttcaaa agttgcctac accctctgct tcaacgccac cagcaggtga
    31741 caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa gatatatgat
    31801 tcgctgtgag gccaaagttt atccagcatc ggcaccactt tgcgcggcac cagaccgtcg
    31861 agatagccat acaatcgcaa aaacggcatg gacacgtttt gcagcggctg acggagatcg
    31921 accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat cggtaacgcc
    31981 agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg tttcagtccc catggtttgt
    32041 aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg ctgaaatccc
    32101 gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca aggtgacgac
    32161 gccacggtga ccagcgcctg aacacgctcg ggatgggtta aaacaatttg gcttgccacc
    32221 agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgttg cagcacggct
    32281 tcggccatat cagcaagtga cagcgcacca aatccccggc tacgcccgaa gccgggcagg
    32341 tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg ccacacttcg
    32401 gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc tttggtctgc
    32461 caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga acatatgcta
    32521 acagtaccgg gattatgctg gctatgccga atgccactgg cgttaggtca ttgggggatt
    32581 tgttcggtct gttcacgcgc cacccgcaca gataaaacgt tatgcccaca atgtggatta
    32641 cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccgcc gccctggcaa
    32701 agactggtca cggttgccga ctatgcgccg ccgttaagtc cgcttatcca ccagcttaaa
    32761 ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta
    32821 cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt tccgttatgg
    32881 cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca gccgttatca
    32941 cgctggttgc actgccaatg ggatagcgaa gccgtcacac gtacacgggc cactgcgacc
    33001 cagcattttc tcagtgcccg gctgcgcaag cgcaacctga aaaatgcctt tcgtcttgaa
    33061 ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac cggaagtacc
    33121 gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc
    33181 ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta aaatagtcaa
    33241 ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact ttgccaaact
    33301 gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc
    33361 taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca ccgacactgc
    33421 cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat acctggaaga
    33481 tgcagagatc gactttgtta ccgaccagtt gggttctcag ttaacgctga aagccccgaa
    33541 cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg gagcgcgtgg agtatatgct
    33601 gcagtcgcag atcaacccac agcttgctgg tcacggtggt cgcgtttcgc tgatggaaat
    33661 caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt gttccatggt
    33721 cgatgtgacg ctgaaagaag ggatcgagaa gcagctgctg aacgaattcc cggagctgaa
    33781 aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact aagttatccc
    33841 ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg
    33901 tgccagacgt tggcacagca ttgctttggc aaatccctcc cttcgtttcc ccgcgataat
    33961 atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa cgccagatgt
    34021 tacccgtatc attcacatgg gtaccaaaca tactcctgac atctgactac aataattagt
    34081 tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga taattagagt
    34141 ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa tcatgccatt
    34201 agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgct tcaaaatgaa
    34261 cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag gaatgccgct
    34321 ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta gccttgccct
    34381 gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg gcgcacaacg
    34441 tatcgccacc acgctgattg ccaaatttgg taaaaaacac atccagtggg cggtggtact
    34501 gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc tgatgctgcc
    34561 gctggtgttt accatcgcgg cttctgcgaa tattccactg ttgtatgttg gtgtaccaat
    34621 ggcagctgca ctgtctgtga cccacggctt cctgccaccg catccgggtc cgactgcgat
    34681 tgccaccatt ttcaatgccg atatgggtaa aaccctgctg tacggtacta ttctggcaat
    34741 cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc
    34801 aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg
    34861 cgtcagcgtc tggacttctc tggtgccggt tgtgctgatg gcgatgcgtg cgattgccga
    34921 aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg gtgacccggt
    34981 aatggcaacg ctgattgccg tgctgattgc gatgttcacc tttggtctga accgtggtcg
    35041 ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg cgatgatgct
    35101 gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa
    35161 atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg cctggtcgat
    35221 tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg
    35281 tatcgcggca ccgctgattg caacgacggg tgttagccca gagctgatgg ttattgcggt
    35341 gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc tgttcaaaga
    35401 gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg aaaccattat
    35461 ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat aacactgact
    35521 gccggatgca tcgcatccgg catcaaccgc actctacttc ttcttcgctg cagctctgcg
    35581 ccgtctgtcc aaatccttca gcaacttgtt cacgccatca tcggcaaaca tcgactcaag
    35641 cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag gaatattcac
    35701 cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac tgttactgtc
    35761 ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc atcgacatca acgatgcctt
    35821 atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca gcccttgctt
    35881 cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat acagccccag
    35941 ggttttgccc agcgttagat ccccgctctc ccagtaaccg cgtagcgtag gcaggtcatg
    36001 tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga acgtcttctc
    36061 gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc gcagcttacc
    36121 gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc gatgacgttt
    36181 actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat acgcgccctg
    36241 atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca catggtcaat
    36301 tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg gttcataggc
    36361 acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc ccaacgggcc
    36421 gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tatagttcac ggtcacacca
    36481 ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc caatcggcat
    36541 ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa
    36601 ccagagataa aaatcgacgt cattacgatg ttcttcgcag aactgacgca cttctggtga
    36661 atccacgttc tgatacatct ctggccatgc aggccagccc cagcgcattt cgtcctcttt
    36721 cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt cgccctgctc
    36781 tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga aacctttcca
    36841 cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga cccagtcggc
    36901 atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc
    36961 ttcgctaaga tggaaatctt cgacggcgtt aacgtcgata taaatcacat tcagccaacg
    37021 gcgagaagac gggctgtatg ggctggcact ctccggattt gccggataga gcgcatgaat
    37081 cgggttcagg ccgatgaacg acccgccacg ttttgccaca tccaccagca tcgctttgag
    37141 atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa gctgaacgca
    37201 ggcaccccac agcttttgtt tattcagcaa ggcctgcggt tcgtaacagc gtttcggggc
    37261 gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgtcagcg tgtgataacc
    37321 ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc ctttgtactg
    37381 tgttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca ccaccatcgg
    37441 catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg ccactttcgt
    37501 ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg
    37561 cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg cggcattatc
    37621 cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac gagcctgata
    37681 atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga ggatcgccgc
    37741 gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg cctcaacata
    37801 ggctgcgaaa tccgccatca ccagatacgg atcgccgcct tgtttgccga tactgtgcag
    37861 catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca gctctttcaa
    37921 tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc ctttggccag
    37981 aatagccttc acttgttcca cggtatgacc aaaaataaag atattttctt caccgacttt
    38041 ctcggcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac cattgagcgc
    38101 cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt gttcggagat
    38161 atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca ggaacaccac
    38221 cttcaactta tcgccaacca gtggatcgtt gttgatcacg tcagccactt tgttgatcgc
    38281 aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga agaggaagac
    38341 gcgcggtacg cgatcagcct gcgggttttc acgaatttct ttgtacaacg ccagaatatg
    38401 cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat
    38461 cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca gacggacttt
    38521 attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag cgaatttttc
    38581 cagattgatc agctgatcga gatcgttagc ccactctttt tgcagtgatt tatccaacag
    38641 agccgccagt gcagggttgc actgtttgat ccagcgacgt ggggtaatac cgttggtgac
    38701 gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt tcaccaccag
    38761 atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac acaggttcgc
    38821 catatgcact tgtttatcgt gcaccaccgc cagtttggcc cacacttttt catcgcccgg
    38881 ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa tctgcatatg
    38941 gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag
    39001 ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat cccagctcat
    39061 ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgatagttg ggtgggtatc
    39121 gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg ccagatgatg
    39181 gcgacgcaaa atatccgcta ccgaacaggc acactggaag tactgctgca tcaagcgcag
    39241 ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt tttccgcatt
    39301 gatgccctgc tgttcggcac gcaggaaatc accgtcgtta aatttagtca gatcaaacgg
    39361 atgcgcgtgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac gatagccgac
    39421 aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt
    39481 cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc ggaaccacgg
    39541 gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcga caaaagactg
    39601 gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac cgacagttgc
    39661 cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca gcgccgggtc
    39721 gatctcttct tccagcaggt ccgtcagatt gatgtcataa gccttcaacg aatcctgtac
    39781 atcctgatac cagccgagat tcaacaggtt gttgcccgtc aggcgaccaa tcaaaaactc
    39841 cattgagatg tagttaacat gtcgctgatt cgccaccggc ttggcgaatg gctgagcacg
    39901 cagcatttcg gccagtgctt cactcactgc tagccaccac tggcgaggag tcatttcagc
    39961 cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa attgcttatc
    40021 gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac acaacgttat
    40081 cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta ggcagggagg
    40141 agttgcgggg atgagcaagg aaatgtgatc tcaaccactt aaagctagtg caaaccacag
    40201 gattagcagc aaatcaatgc aatacagcgc agaaaatctg tatctaagtg caaaaaatgg
    40261 ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag gagtgaaaca
    40321 gtttcataag taaaatatcc agtgtgctcc atctcattct taatagattt attaagatca
    40381 tctttttaga tagcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga
    40441 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
    40501 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
    40561 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
    40621 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc
    40681 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
    40741 acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt
    40801 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc
    40861 agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc aacggtcact
    40921 gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg
    40981 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
    41041 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
    41101 cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc
    41161 tgcgtgttcg tgaccaactg ctggaaattg gcagtcagca actggcattt acccatcagg
    41221 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca gaaagcagtc
    41281 ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc ctctccgccc
    41341 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
    41401 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
    41461 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg
    41521 gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc
    41581 ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc
    41641 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
    41701 gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
    41761 cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac
    41821 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
    41881 agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca
    41941 ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag
    42001 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac
    42061 ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc cgcattgtgg
    42121 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
    42181 taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga
    42241 gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
    42301 cacaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg ccgatgcatg
    42361 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
    42421 aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc
    42481 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
    42541 gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
    42601 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
    42661 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
    42721 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
    42781 ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
    42841 acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
    42901 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagccat tttgtcatcg
    42961 aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg ctgccggaac
    43021 tggaacagca tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat cggcataaat
    43081 tcgcccattt cgatgagaat ttcgttgaac gtctgctgaa tcatccggaa gtgccagaac
    43141 tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atttactccg
    43201 gttacagcaa tgagcaaata gcgggtgaac tggaagtggc ggcaaccacc atcaagacgc
    43261 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg
    43321 cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg
    43381 atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc
    43441 aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat
    43501 gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag taccagctgg
    43561 tcggcgagat attcccccac tgccgccggg cttgccaggt agcgtttcac ctctttcact
    43621 aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacaaa aaagcgttcg
    43681 gtgatatttt cactttcgac ttcaagcgag acggtattac ccggcccctg gtcacgcggc
    43741 aggttatgaa tattctgctc atgcagggaa aaactacccg ccagcgtagc gatttcacgc
    43801 tcagcgacat ggcgcggcac gccagctaat aacacctctc cacgcatctg cacaatgttc
    43861 ccgcgctcgc caagttgcaa ggtgttaaac gatgccaccg gcgagacttc cgttgccacc
    43921 acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
    43981 attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg ggccgacggg
    44041 ttatcggtgc ctccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
    44101 agcaccgttt gcagcaccag cgtacagctt ccggcactac cgatagcaaa gcggtactcg
    44161 ccgccgcgca cggtgccagg ccggaagacc agacgctgcg accccagctc cgcgccttcc
    44221 accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg gcgcaacagc
    44281 cccggtttcg cccgtccggc tcgaatgcca gtgatggtaa atggctggcc ggttatcatc
    44341 gacaggctca gcgccgagcg caggatctgt ccgccgcctt cgccctgtgc gccatccagc
    44401 gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag ggtgtagata
    44461 acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata cgccatcggg
    44521 atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc acgaatttga
    44581 tcttccacgc tgaacagttt tttcgcttta gtacggctca ttacccgccc ggcaccgtgg
    44641 ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct ttttgctccc
    44701 atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc tttacgcgtc
    44761 acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg acagttgatc
    44821 tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaacgtc
    44881 gttaccacat tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg cgcccaggcc
    44941 acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc cagatcacgc
    45001 gacggcaacg tctcaagttg gtcctgcatc tctttttgcg ccagatcgat aaagtaagtc
    45061 ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac ctgctccgac
    45121 tcatcaaggc agatttcaat aaagtggtta ccggttccca gcgttcccag gtgtttatag
    45181 ttattggtat tcaggaaacg gggatatttt tgcgttaacc actgataacc ggcttccagc
    45241 tcagcccatt tcgcatcgac gttaacaggc gggttttccc aggcaccttt atcacgttta
    45301 caacggccag tggtacgccc gtgcggcacg gccgtttcaa tcgcctgacg cagctctgcc
    45361 aggttttcag gcaggtcttc cgccgttaac gcggtacgca gcgcgttcat tccacagcca
    45421 atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg
    45481 gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc
    45541 atcttcgccg tattaataag ttgctgacgc gcatcggcct ctaccggcac gcctttggtc
    45601 cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct
    45661 tttcgt
//
LOCUS       DAZFAH010000016        44934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFAH010000016 DAZFAH010000000
VERSION     DAZFAH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.016"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHJWB_000832"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHJWB_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4312512.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHJWB_000833"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHJWB_000833"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4312513.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHJWB_000834"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHJWB_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4312514.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHJWB_000835"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHJWB_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4312515.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQPPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHJWB_000836"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHJWB_000836"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4312516.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHJWB_000837"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHJWB_000837"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4312517.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHJWB_000838"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHJWB_000838"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4312518.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHJWB_000839"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHJWB_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4312519.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="ACHJWB_000840"
     CDS             complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="ACHJWB_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4312520.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9293..10138
                     /gene="glpF"
                     /locus_tag="ACHJWB_000841"
     CDS             9293..10138
                     /gene="glpF"
                     /locus_tag="ACHJWB_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4312521.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10161..11669
                     /gene="glpK"
                     /locus_tag="ACHJWB_000842"
     CDS             10161..11669
                     /gene="glpK"
                     /locus_tag="ACHJWB_000842"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4312522.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="ACHJWB_000843"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="ACHJWB_000843"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4312523.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="ACHJWB_000844"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="ACHJWB_000844"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4312524.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHJWB_000845"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHJWB_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4312525.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /gene="yiiS"
                     /locus_tag="ACHJWB_000846"
     CDS             complement(14117..14416)
                     /gene="yiiS"
                     /locus_tag="ACHJWB_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4312526.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /gene="yiiR"
                     /locus_tag="ACHJWB_000847"
     CDS             complement(14628..15068)
                     /gene="yiiR"
                     /locus_tag="ACHJWB_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4312527.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /gene="yiiQ"
                     /locus_tag="ACHJWB_000848"
     CDS             15169..15768
                     /gene="yiiQ"
                     /locus_tag="ACHJWB_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4312528.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHJWB_000849"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHJWB_000849"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4312529.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHJWB_000850"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHJWB_000850"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4312530.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHJWB_000851"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHJWB_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4312531.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18870..19832)
                     /gene="pfkA"
                     /locus_tag="ACHJWB_000852"
     CDS             complement(18870..19832)
                     /gene="pfkA"
                     /locus_tag="ACHJWB_000852"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4312532.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20013..20915)
                     /gene="fieF"
                     /locus_tag="ACHJWB_000853"
     CDS             complement(20013..20915)
                     /gene="fieF"
                     /locus_tag="ACHJWB_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4312533.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21152..21370)
                     /gene="ogrK"
                     /locus_tag="ACHJWB_000854"
     CDS             complement(21152..21370)
                     /gene="ogrK"
                     /locus_tag="ACHJWB_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO4312534.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21452..22615)
                     /locus_tag="ACHJWB_000855"
     CDS             complement(21452..22615)
                     /locus_tag="ACHJWB_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO4312535.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIATRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGRGVTASGKKIPQVIITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDEE"
     gene            complement(22615..23094)
                     /locus_tag="ACHJWB_000856"
     CDS             complement(22615..23094)
                     /locus_tag="ACHJWB_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4312536.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23109..25556)
                     /locus_tag="ACHJWB_000857"
     CDS             complement(23109..25556)
                     /locus_tag="ACHJWB_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4312537.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRDTQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGQELKKARQEAAALAVQFKNTERPTNAQAKAMEAARK
                     NASELQAKYNSLRLSVQRQRQELSQAGINTRNLAHDERGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVAKTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALTGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25549..25704)
                     /locus_tag="ACHJWB_000858"
     CDS             complement(25549..25704)
                     /locus_tag="ACHJWB_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4312538.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25701..25976)
                     /locus_tag="ACHJWB_000859"
     CDS             complement(25701..25976)
                     /locus_tag="ACHJWB_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4312539.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26033..26551)
                     /locus_tag="ACHJWB_000860"
     CDS             complement(26033..26551)
                     /locus_tag="ACHJWB_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4312540.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26564..27754)
                     /locus_tag="ACHJWB_000861"
     CDS             complement(26564..27754)
                     /locus_tag="ACHJWB_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO4312541.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(27814..28407)
                     /locus_tag="ACHJWB_000862"
     CDS             complement(27814..28407)
                     /locus_tag="ACHJWB_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4312542.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTASEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
     gene            28438..28848
                     /locus_tag="ACHJWB_000863"
     CDS             28438..28848
                     /locus_tag="ACHJWB_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4312543.1"
                     /translation="MPVTRYLCIFINVGLGEAAKRNVGTGENQIPDMASFASGDGWMK
                     LPNGKILQYGRGEAMPKLSTQTMRITFPIPFPKKADIAILTHSGDGGAPFGAGRGFVM
                     SVEGPTLTGFNSAYRTASTSDKVSMTYSWWAVGE"
     gene            28869..29312
                     /locus_tag="ACHJWB_000864"
     CDS             28869..29312
                     /locus_tag="ACHJWB_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4312544.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVSQNIISEFM
                     AEPPQGKIRIAGGDGLPAWADIPAPTHEELIEITESERQLLINQANDYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIKWPTPPAVQAR"
     gene            complement(29284..29886)
                     /gene="tfaE"
                     /locus_tag="ACHJWB_000865"
     CDS             complement(29284..29886)
                     /gene="tfaE"
                     /locus_tag="ACHJWB_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4312545.1"
                     /translation="MDNAVLNSEFIATKAGNITVYNYDSETREYISTSTEYLAVGVGI
                     PACSCLDAPGIHKVGYAICRSVNLNSWEYVPDHRGEIVYSTETGESKEITAPGDYPEN
                     TTTIAPLSPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYTDAVTATDTSTAPDVIWPELPEA"
     gene            complement(29886..31385)
                     /locus_tag="ACHJWB_000866"
     CDS             complement(29886..31385)
                     /locus_tag="ACHJWB_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4312546.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDEVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPDAGNNTTRIATTAFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFSTTVLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFDKVKYPRLA
                     TAYPSGKLPDLRGEFIRGWDDGRGIDTGRALLSIQSDEVRKLALKYWGPASNSSPSKT
                     FALSDSAGGGLYTDGISQASGGIINAFQLPGGNETRPRNVAFNYIVRAA"
     gene            complement(31382..31993)
                     /locus_tag="ACHJWB_000867"
     CDS             complement(31382..31993)
                     /locus_tag="ACHJWB_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO4312547.1"
                     /translation="MSDSRLLPTGSSPFEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDNWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(31986..32894)
                     /locus_tag="ACHJWB_000868"
     CDS             complement(31986..32894)
                     /locus_tag="ACHJWB_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO4312548.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAAVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNNTQASFCTEYSVVTGGSDE"
     gene            complement(32899..33246)
                     /locus_tag="ACHJWB_000869"
     CDS             complement(32899..33246)
                     /locus_tag="ACHJWB_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO4312549.1"
                     /translation="MTARYLGMNRSDGQTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(33243..33878)
                     /locus_tag="ACHJWB_000870"
     CDS             complement(33243..33878)
                     /locus_tag="ACHJWB_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4312550.1"
                     /translation="MNTLANIQELARALRNMIRTGVIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(33945..34397)
                     /locus_tag="ACHJWB_000871"
     CDS             complement(33945..34397)
                     /locus_tag="ACHJWB_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4312551.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQAAMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(34390..34857)
                     /locus_tag="ACHJWB_000872"
     CDS             complement(34390..34857)
                     /locus_tag="ACHJWB_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4312552.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPVTRPMELYINGELVSKWDE"
     gene            complement(34820..35065)
                     /gene="lysC"
                     /locus_tag="ACHJWB_000873"
     CDS             complement(34820..35065)
                     /gene="lysC"
                     /locus_tag="ACHJWB_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO4312553.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(34965..35390)
                     /gene="lysB"
                     /locus_tag="ACHJWB_000874"
     CDS             complement(34965..35390)
                     /gene="lysB"
                     /locus_tag="ACHJWB_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO4312554.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     ATITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(35378..35803)
                     /locus_tag="ACHJWB_000875"
     CDS             complement(35378..35803)
                     /locus_tag="ACHJWB_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO4312555.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTALFLQPVPWRRRSVALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(35818..36315)
                     /locus_tag="ACHJWB_000876"
     CDS             complement(35818..36315)
                     /locus_tag="ACHJWB_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO4312556.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     QEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(36315..36596)
                     /locus_tag="ACHJWB_000877"
     CDS             complement(36315..36596)
                     /locus_tag="ACHJWB_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4312557.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKSRGKP"
     gene            complement(36600..36803)
                     /locus_tag="ACHJWB_000878"
     CDS             complement(36600..36803)
                     /locus_tag="ACHJWB_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO4312558.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(36803..37312)
                     /locus_tag="ACHJWB_000879"
     CDS             complement(36803..37312)
                     /locus_tag="ACHJWB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4312559.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(37412..38155)
                     /locus_tag="ACHJWB_000880"
     CDS             complement(37412..38155)
                     /locus_tag="ACHJWB_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO4312560.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(38159..39232)
                     /locus_tag="ACHJWB_000881"
     CDS             complement(38159..39232)
                     /locus_tag="ACHJWB_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO4312561.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(39287..40141)
                     /locus_tag="ACHJWB_000882"
     CDS             complement(39287..40141)
                     /locus_tag="ACHJWB_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO4312562.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMENAFSALKQDVTDRADETSQAFTCLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            40315..42087
                     /locus_tag="ACHJWB_000883"
     CDS             40315..42087
                     /locus_tag="ACHJWB_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO4312563.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            42087..43121
                     /locus_tag="ACHJWB_000884"
     CDS             42087..43121
                     /locus_tag="ACHJWB_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4312564.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFATGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDALQDR
                     DYIDSIRENMANSKGRNNFKNLFLYAPQGKPDGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            43334..43603
                     /locus_tag="ACHJWB_000885"
     CDS             43334..43603
                     /locus_tag="ACHJWB_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312565.1"
                     /translation="MQMHEHAEGAPVPAALTRDWEGKRMRFDAVMHAELKMAEHTEKT
                     HKKTGIQVAGLSRLHCGYWPRNAPIILVRQQVATISKKTVVHGFS"
     gene            complement(43634..43828)
                     /locus_tag="ACHJWB_000886"
     CDS             complement(43634..43828)
                     /locus_tag="ACHJWB_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312566.1"
                     /translation="MHNSLSSIKDPYADWAKRLTVMASNNDLSSREVESYTEKMVEQA
                     SKDELTVVIKHLLNHIRMNK"
     gene            43827..44264
                     /locus_tag="ACHJWB_000887"
     CDS             43827..44264
                     /locus_tag="ACHJWB_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312567.1"
                     /translation="MSKNLELFNQQTAEIFAVLWDNFPVPQVITYKKFNAALPDDYFD
                     QLNSPEMKALNQLRSVVDGTFTFLSENGYILYGTDHQTGFHDVRLTEKALAVLNKKPE
                     ALGGNETMGDKIISAVKDGTPGVIAGAVTNLLTLGVNFVTSAS"
     gene            complement(44389..>44934)
                     /locus_tag="ACHJWB_000888"
     CDS             complement(44389..>44934)
                     /locus_tag="ACHJWB_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFO4312568.1"
                     /translation="SFFFSVMKMSSDVADVLSHICTCHDRLPTGSRISMPLAYFANSR
                     MFGEIYQLCQKLRVNMTVYVDDLTFSGSNVNRLFCAVIRKIVNKHGHVIHPTKTKLYA
                     RDKPKLVTGVIVLGNALKVRNEQHFLMAREIDYWKIIKDAENARETIIAKKLFGRLHS
                     MGVIEQRYKSKVLTLKANTAN"
BASE COUNT        10488 a        12222 c        11626 g        10598 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagccgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta
      721 gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt agttgaagtg ctggacgttg
      781 agccggtatt tactcactcc gtctggcggt tactgctctg ggcggcagat tactatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggtcaggcg gtggatctta
      961 acagcctgaa acgttcgcca aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa
     1021 tctggcgcga ccaggtcgcg gaacttgaat ttaatgatgc cgcgttgcag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca agccaccgcc gttggtgcaa
     1201 ttcatagcgc ggcagatact ttttctgcct ggctgctggc gggcgttacc ggttccggta
     1261 aaacggaggt ttatctcagt gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt ggaagttctg cattccggcc tgaacgacag cgagcggctt tcggcgtggt
     1441 tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggcgtg attgtcattg atgaagagca cgacagctcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacgcccg cgctggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacccgtc gggcagggaa tgcgcgtccg gcaattcaac
     1741 atgtgctgga tttaaaaggt cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccaacaatc aggttatcct ctttcttaat cgtcggggtt
     1861 ttgcgcctgc gctgctgtgc cacgactgcg gctggatagc cgaatgccca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagtcagc
     1981 gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtcccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgcttgcgc cgatgttccc cggcgtgccc atttcacgta
     2101 tcgaccgcga taccaccagc cgcaaagggg cgctggaaca gcaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatcttg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagaacg tttcgcccag ctttacaccc aggtcgccgg tcgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcacc atccggaaca tcctctgttg caaacgttgc
     2401 tctataaagg ctacgacgcc tttgccgaac aggcgctggc tgagcgacgc atgatgcagc
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctttccag cccgctggca gacgacaaac
     2581 tgtgggttct cggcccggtt ccggctctgg cacctaagcg tggtggccgc tggcgctggc
     2641 agatcttgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg
     2701 cgcttattaa tacaataccg gattcccgca aggtgaaatg ggtgttggat gtcgatccca
     2761 tcgaaggcta atgcgcaaac gttgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 tgtctcccga gcattaatga atcccgataa agtctcccag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt cgcaccggaa ctggagctac ctacagttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ctatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggta
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat taaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacaacgcca gcgtcaggcg cagcaattag cggagcagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaac
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggat
     4861 ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc tggcgaaaga
     5341 ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag aaaatactga
     5521 actgagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcgt ctgccaatcc gcgttgaact gcaggcgctg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaagcgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaactatca tcattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacag cgctggcatg
     7141 gtggcaaggt cacttcgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac tggttgcagt
     7381 ggcatgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttgag tgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaatatt aataacctgc gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 gctggtgtgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccattac tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccaatgc tggaacgtac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgtttta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag cccacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttctgaagat
     8521 ccgctggata ttgaatgata gaaaaggcac cgcaaggtgc cttttttctg cgttacctgt
     8581 tggcctacac agtaaagaaa ttacgcggaa gatgaagcgt aatcagacct cttccatgcg
     8641 acccagcagg gcctgcagac gttcctgcca gccgttctgc tgttctttca gatggttgtt
     8701 ctcacgctcc agctcttcgc gctgatgctg ggcattttga acttcctgcg acagtgagtt
     8761 gtttttttct ttcagctctt cgatttccat ctgcaacaga gtgatggtat caatcgcctg
     8821 ctgtactttt gcttccagtt tctcaaacac ttctaatgac attgtcatac ctctcctgaa
     8881 ttgcaaggcg ttgatggata aaaatcctcg tcccgattac cggtgacgcc ttaataaata
     8941 cgagcgcact ttagttagct ccgattgtat gaagccgcgc catcgctgtc cagcggcacg
     9001 ccttgcagat tacggtttgc cacacttttc atccttctcc tggtgacata atccacatca
     9061 atcgaaaatg ttaataaatt tgttgcgcga atgatctaac aaacatgcat catgtacaat
     9121 cagatggaat aaatggcgcg ataacgctca ttttatgacg aagcacacac attttaagtt
     9181 cgatatttct cgtttttgct cgttaacgat aagtttacag catgcctaca agcatcgtgg
     9241 aggtccgtga ctttcacgca tacaacaaac attaactctt caggatccga ttatgagtca
     9301 aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc ggtaccgggt tgttgatttt
     9361 cttcggtgtg ggttgcgttg cagcactaaa agtcgctggt gcgtcttttg gtcagtggga
     9421 aatcagtgtc atttggggac tgggggtggc aatggccatc tacctgaccg caggggtttc
     9481 cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa
     9541 gcgcaaagtt attcctttta tcgtttcaca agttgccggc gctttctgtg ctgcggcttt
     9601 agtttacggg ctttactaca atttattttt cgacttcgag cagactcatc acattgttcg
     9661 cggcagcgtt gaaagtgttg atctggctgg cactttctct acttacccta atcctcatat
     9721 caattttgtg caggctttcg cagttgagat ggtgattacc gctattctga tggggctgat
     9781 cctggcgtta acggacgatg gcaacggtgt accacgcggc cctttggctc ccttgctgat
     9841 tggtctactg attgcggtca ttggcgcatc tatgggccca ttgacaggtt ttgccatgaa
     9901 cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc
     9961 ctttaccggc ggcagagaca ttccttactt cctagtgccg cttttcggcc ctatcgttgg
    10021 cgcgattgta ggtgcatttg cctaccgcaa actgattggt cgccatttgc cttgcgatat
    10081 ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa caaaaagctt cgctgtaata
    10141 tgactacggg acaattaaac atgactgaaa aaaaatatat cgttgcgctc gaccagggca
    10201 ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa tatcattagc gtgtcgcagc
    10261 gcgaatttga gcaaatctac ccaaaaccag gttgggtaga acacgaccca atggaaatct
    10321 gggccaccca aagctccacg ctggtagaag tgctggcgaa agccgatatc agttccgatc
    10381 aaattgcagc tatcggtatt acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa
    10441 ccggcaagcc tatctataac gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg
    10501 agcatttaaa acgtgacggt ttagaagatt atatccgcag caataccggt ctggtgattg
    10561 acccgtactt ttctggcacc aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg
    10621 agcgtgcacg tcgtggtgaa ttgctgtttg gtacggttga tacgtggctt atctggaaaa
    10681 tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc ctctcgtacc atgttgttca
    10741 acatccatac cctggactgg gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga
    10801 tgctgccaga agtgcgtcgt tcttccgaag tatacggtca gactaacatt ggcggcaaag
    10861 gcggcacgcg tattccaatc tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc
    10921 agttgtgcgt gaaagaaggg atggcgaaga acacctatgg cactggctgc tttatgctga
    10981 tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg
    11041 gcccgactgg cgaagtgaac tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca
    11101 ttcagtggct gcgcgatgag atgaagttga ttaacgacgc ctacgattcc gaatatttcg
    11161 ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc ggcatttacc gggctgggtg
    11221 cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg
    11281 ctaaccacat tatacgcgcg acgctggagt ctattgctta tcagacgcgt gacgtgctgg
    11341 aagcgatgca ggccgactct ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag
    11401 tagcaaacaa tttcctgatg cagttccagt ccgatattct cggcacccgc gttgagcgcc
    11461 cggaagtgcg cgaagtcacc gcattgggtg cggcctatct cgcaggcctg gcggttggct
    11521 tctggcagaa tctcgacgag ctgcaagaga aagcggtgat tgagcgcgag ttccgtccgg
    11581 gcatcgaaac cactgagcgt aattaccgtt acgcaggctg gaaaaaagcg gtcaaacgcg
    11641 cgatggcgtg ggaagaacac gacgagtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ctgaaacgtg cgggggcaac cccgcacaca tcaataatcc cacccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg
    12181 gccgggagcc aaaggcacca ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 agcggcggcg ctcggcaaac cgttgagcga actgacggta acgattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcagca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgtctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gtattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tccatccact atctggatcg
    12781 caaagacccg gaaatgcagg tgcacattct ctgagtgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgatgat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgattggcg gcactgaaac cgggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gatgaagtac cggactgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccgggc ggataccggc gttaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtgatgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gagctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tgtaggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctgg gtatctttac catatttatt ttcgccttgt
    14701 gtgccaatga acgctccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acggttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ccagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctgct aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccgcgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ccacctcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcca ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccgcg ttccgcaatt gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaaagatt ggctataact tgcgggtata tgttgaggga
    18661 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
    18721 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat
    18781 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
    18841 gtaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
    18901 tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc
    18961 tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg
    19021 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
    19081 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
    19141 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
    19201 gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaacc
    19261 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
    19321 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
    19381 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
    19441 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
    19501 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt
    19561 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaagcgcgca
    19621 gaaccgagga acgtaccgcc acggttgatc atgtcagaaa cgctgtaacg gtctagctgt
    19681 accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac ttccagacct
    19741 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
    19801 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
    19861 taaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag
    19921 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
    19981 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
    20041 ctaggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg aaaacgccgt
    20101 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
    20161 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
    20221 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
    20281 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
    20341 tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac
    20401 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
    20461 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaacg ctgaaacgag
    20521 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
    20581 gtcatcggtg ttggagatat cagatgttga atacccgtca aaaacaggaa tagtgccgaa
    20641 ccggagataa acatactttg cgccagcgcc gcgagggact ctgctttacc gtgaccaaac
    20701 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
    20761 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
    20821 caccatgcaa aaattttaat cagcaatagc aacgaagcca tcgccgtcgc agcaatcgcc
    20881 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgc
    20941 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
    21001 aaaaacccca catcatgtgg gggaagacag ggatggtgtc acaaaaaagc acctaacctg
    21061 atgatataaa tggatttata tcaagcactg tccacatagc gtccacatcg accataaata
    21121 aagcccctca actgaggggc tatttttgtg atcacatcca cataatttgc tgccctgacg
    21181 gcaacgggtg cggcctcacg gcgtggactt ctcccggctt cacgatgtat cgctgtaccg
    21241 actcataagt gatgaacgtg gcgctgcaat tcacgttctg acactggtga taacgctctt
    21301 ttgtcgtgtc agtgatatag cgacttgtac gcgcatgtgc ggcatgctgg cataaaggac
    21361 aatgaaacat cgcgagcacc tcttccggtt ttgttgatgg tgccatttta gttaatttat
    21421 tcttacaaaa caaacagata aataaaacac atcattcttc atcttctgtt tcgtactcca
    21481 catcagaaag cctgacctca agctccaagg acgtcgtgaa gccgctatta ttcagaaaat
    21541 gtgtcacctt agtgattgtc cagtcctgct cgtctatgac gcgcttaaag cctgacactt
    21601 tgaccggcgt ttccgtgtaa atatctgcac gaccggtagc caggctgatg gagaactccg
    21661 caacgccccg ttgcagctta tcccacttcg cctgagcggc gcgcatggcc tgcgctttcg
    21721 tggcataaac cgtggtcagg gcaaaaacgt tgtcagcctc accggccatg tattcacctt
    21781 cgcgcgcttc cggtactttt ggcgctttct tctgcgtgac cggtttcgct ttggggtgct
    21841 ccagtgcgcg caggtgtttt tctttctttt tgcgtttcag ttttaccttc tgcttttgag
    21901 gcttcgggtc tttagtgtgt agccattttg ccgttacacc ggtgtaggct ccacggtcag
    21961 caatcgcaaa atgatgacgg tcgccgtcgc tgcgggtgat gatgacctgc gggattttct
    22021 taccgctggc cgtcaccccc cgccccgcct taagaaataa cagttttccc atttttaccg
    22081 acacctcacc gccgttgcgt tcagcaaggc gggtcaggaa ttttgcatca gactcctgcg
    22141 actggtcgat gtgcgggatt ttaattccgg ccagcgacgg agcgacactg gcttccagcc
    22201 tgttacgggt ggctatcgcc tcaacaatcg caccaagcgt ggtgtcatgc caggagcctt
    22261 cccggcggga attgagcgtc ccgcgaaaat ctgcactccg ggcgcggatg gtgaccacat
    22321 ccggcgcgcc ccggtgttca acctcatcaa cggtaaattt ccctttgcat accagggcaa
    22381 aacctttcca gccgatatac accgtcagga cagcgccacg aaccggcagt ccgacctgcc
    22441 cgtcggcatc gttcagttca atatcaagct ggtcagcctc aaagccccgg ttatccgtca
    22501 gagtcatgct catcagacgg tcgctgatat tgccggtaat atccctgctg tcgagcatca
    22561 gcatgtaatc cggcgtcagc gtactgcctg catcaaatgt cagcgcatcc agcattatcc
    22621 cgcccccgtc atacccgtga atttagtcgc catactgcca gccttaccga tgagcgattc
    22681 cgcctgttta ccgatatcgc cataaagcgc ggccagtgat tcatcaacgc gggtgagcga
    22741 cagcgtaaaa tcaatttttc ggggtgtgcc gtctgcaaag aaaatactcc ctgtttcact
    22801 caccttgctg atgacataca tgccgtaaat catgccggta ccatccagca acggccacgc
    22861 ccggccttcc tctgccatca gtctgagcgt ggtcatcgtc agcttgccgc cggtcagttc
    22921 gggataaagc acaccggcaa gcgtcatgtt ttcctcacca acgccgagaa actggtaggc
    22981 atcccgttta ccgatacggg aatttgacgg ccagcgataa tctgattcac gctgcatggt
    23041 ctggtgtggc agcgtctggc gcataaaaac aaacatacct aacgcgagca tcatttttcg
    23101 tcacctcctt aaccgtcatg catcatgctg gcacgggcgc gcgcacgttt atcccgctcg
    23161 tatttttcga gcgcatcctg taactggcgg tcaagctgtg tccccggtgc agtaccaccc
    23221 gtcaggctga tgtgatattc gtttttactc tggtccacat aagagcggcc agccggtgcc
    23281 gtgaccggct gataagcctg atagcctgca taagagctgg tcgccggaat ataaccaccg
    23341 gtgccatacg tggcggcatg agtccttgcg gcggtctggt caagtgtgtc tgactctttg
    23401 ttgataaccc cgagtttttc cagtacccag tctataccgc tgcgcagttt gttgaacgca
    23461 ttaagcggca gcatcagcgc gtcagccagt gcctgcccga acatgacgcc cgtgtcacgg
    23521 caacggttca gggtgtcctg ggtggctttg accggggcaa tcaggttttt aaaccactgc
    23581 cacgcggcct gtaacttttc gcccagccag tcaaacaccg gcttaagtgg cgtgaacagt
    23641 tctcccaccg gcgcaaatgc cgctttcagc ccttccacca caccgccaaa gaatgcgctg
    23701 acaggctccc agtatttacg gataagcaac gccccggcga caattgcagc caccacggcc
    23761 acaaccggcc agctaatcgc cccgatggcc gtcataacgg cactaccaac cgtcgtgaag
    23821 attgccccca ttgcgcctgc tgccgcgatg atggcattga tgccggtgat aaccggccag
    23881 gctacgaggc caatggcacc gatgacacca gtcagcgcca gtgcaccacc gacaatgagg
    23941 ccgatggttg acgccagtga tttgtttttc tggatccagc cgtcgagttt taacacatac
    24001 tttgtggccg tctgcgtgag attacgcagt gcgccttcct gctggtcaaa caggtcagtc
    24061 cccaccgcct cataagcgga ctgaaactcc ttaaagtcac cgccgaggtt atcctgcatg
    24121 attttaacca gctcttccgt tttaccgtcc gaggctttca gcgtggcggt cagcttatcc
    24181 agttttccgc ttgctgccgc tgccagtaaa acgttcgctg atttcagggc ttcctcacca
    24241 aaaatggttt taaggtattc ccccttctga gacgttccca gcttgtgttt atcaaagctg
    24301 gcctgaatct ctttcagaat ggtgaacaac ggacgcatat ttcccttttt gtccgaggtt
    24361 ttaacgccaa gctctttgag tgcatcccat gcttttccag tcggtgcctg taatcgggtg
    24421 acaacggcgc tgctacccgt ccccgccatt gaccccctga tgttattgtc atgcagcaca
    24481 cctgtcatgg ctgctgcctg ttcaagactt acacctgccg tcttcgcaac cgggccgagg
    24541 taagtcagtg catcactgag tccctgaaaa tcagccgccg acttattcat cgttgccgac
    24601 aacacgtcgc ccacatggct gacatcatca tttgacagtt gaaacgatgc cttagtcccc
    24661 agcaacagtt gcgcgttttc ttccatcgac cgctgattcg ccagtgccat attcagcgtg
    24721 accggcgttg ccgcctgaat agccgcagca tctccacccg ctttcgcaat aataatctgc
    24781 gcaccggccg catcatccgc cgaggcggcg gtattgtcgc cgagctggcg cgcctgtttg
    24841 cgtaatgcgg ccatttcggt ggagtctttt gccacaccta gcacggcctg taattctgag
    24901 tttttctgcg caaactcata accgggcatc agcagcttaa ctccggccat cgttcccgcc
    24961 gccgcaatcc ccacaccggc agcgcctact gaggccatat ttccggccag ttcctttcct
    25021 gcctgataac gctgtttgac tgcgttaagt tttgcctgtt gtgcactgac acgcgccagc
    25081 gcgtcacgct gacggttaag ctgggcggtg gtttcactga tacggttttt cagccctcgt
    25141 tcatcatgcg ccagattacg ggtattaata cccgcctgac tcaattcctg acgctggcgc
    25201 tgtaccgaca atctcaggct gttatatttc gcctgtaact cagacgcatt tttacgcgcg
    25261 gcttccattg cctttgcctg tgcatttgtc ggtcgttcag tatttttaaa ctggacagcc
    25321 agagctgcgg cttcctgtct ggcttttttc agttcctgac cagtcacggc gagctgtgca
    25381 ctggtcttgc gaaatccctc aatacgggat gcctgaccgt tcagctcgcg cagtgatttt
    25441 tgtgtgtccc ggatatcccc cgacagcgac ttgctcgctg tacggatgga tttaaacggg
    25501 cgggatgcct ggtcaacagc cctgagcaat acctgtaatt ttacattgtt actcattcgt
    25561 gtttccgctt cgccggagcg ccttttcgcg ccatgtgatg agttcggtca ggctcatggg
    25621 atacagttct gatggcggcc agtgaaatat cactgccaca tccgccatca ggtcatcgac
    25681 cgacagattt ttcggaaacg tcactgcacc gagttcggcg acaaaaaacc gaccacctta
    25741 ccggccagcg ccacaaggtc aggcagttcc agcgcggcga cttcctgctc ggtcagcatc
    25801 ggtgccgtca tgcgcggcag caccttaatc agtgcatcga cttcggagtt cgcgaccgca
    25861 gccagactga caccgcgcag cgtcccggca ttaggtttca tcagcgtgac ctgttcgata
    25921 acctgctcac cacgtttaac cggattgtcc agggtaatga cattttcttt gttcatggtt
    25981 ttctcacttc tgaatcaggg ttaaccggtc agccaggctg accggatgaa aatcacaggc
    26041 cgatattgcg gcggtgttgc tccagccggt cgacgccgtt caccttctca atcatgttga
    26101 tggtgtcgat ttcgaccagc tccttaccgt ccatcgtcag ccggaaatag gtgcagacca
    26161 cggagatttt cgactcggtg tcttctccct gtttaccctc gccggtgtcg atttctttct
    26221 gacgtccacg catgaccacc tcgacggcca ccgtttcgcc ggtatcgtcg cgctggtaag
    26281 agcctgcaaa acgaatcggt acggcatcca caccggttgc ggcgtaaagc tcccagataa
    26341 ccgaatcagg gaagccaccg agcgaccact ccattgacag cgcatcgtca tcaaggccga
    26401 ggtctaccgg tgcgctgcca ttcatccccg caccgcgata gttttcgagc ttacgggtca
    26461 gttttggcag cgtgacggac tttgcaacgc cctgatagct gtagccgttc agaaagacgt
    26521 tcattaactt gagtttgcgc ggcattgcca tcggtcaggc tccttaattg ctgttaaccg
    26581 aggtgaccag atttgccagg tatttatcgg tgatacgctg gcgcagggtc aggttttcaa
    26641 gaggaggcac cggtgtatag tcgtagtcga tatacagttt tccggccttg agggtttccg
    26701 catcgttgga ttcttcgctg aaccagcagg tcgcatccac gatatagccg tttgttttca
    26761 gctcacggaa tttggcattg atgccgtcaa cgatgtcacg aatcagcgtt gcggtgatgg
    26821 gcttgtccac cgcccacatg tgcgcctcag ccatcgtgtc ggccagcacc tgcgcggtgc
    26881 gggtgtagtt ttcaaagagg aacagcgggt catcagagca ggtacggtta ccccagaagc
    26941 ggaaaccgtc gcggcgaatc agcgtagtga cgcctgactc gttaagcagg tcagcatcgg
    27001 tgccggactc ctgcaaatcc cagaagacgg atgcgctgat gccggtaaca ccgttcaccc
    27061 cgacattgga cagcgtttta tgccagccct gctcctggtc gattttagcg cgcagaccca
    27121 gcgcacgggc ggtggcatac gcggtggcgg tggtactggc gaccgtatcc catgcgagga
    27181 aatccggcca gatgaccatc agctcacgct ggctgaaatt ctggcggtag gctttcacct
    27241 cggaaatggt cttacatccc catgcgctga tatacccgaa agcacgcagc ttctgacaga
    27301 ctgatgccag tgcaacagcc acctctttgg tgtccagtcc cggcacgccg agaatacgcg
    27361 gtttaacacc agttaccgac tccgccgcca gcagggcttt cagtccggtg tactgaccgt
    27421 tttcgtcggt ggtgccgatg atattggaaa cggtctgcgc gagttttgtt tcctcgtcgt
    27481 cgccggtgcc gtcttccaca cgcacgacaa cggtgaccgg ttttgactgg tcagcgatgg
    27541 cctgcaacga cgccgccagc gtgccttttt taccggcctt tgcaattgcg ctttgcacat
    27601 tggtaatcag caccggttta ttgaggggga aggtttccgc atccgcatcg ctggccgtgc
    27661 agaccatgcc gacaatggca gtggatacgg tggaaatgac gcgggtgccg tcgttaatct
    27721 ccagcacctg cacgccgtga tgatagtcac tcatccgttt aactccgtgg ttaatgggtg
    27781 agtggtattt tcagttgtgc cggagatgtc aggctatttg tcccggttgg ctaagggatg
    27841 acacaattta ttctttgtcg ctgatgaggg gaatttttta tagagcgtgg acaggccaat
    27901 atcaaaaatc agcgccacac gttgacgaga ttcccccgca gccagcaacc gtccgacctg
    27961 ctcccactca ctcgcggtga gtttcggtcg tctgccacca atacgacctt tggctctggc
    28021 cgcttccagc ccggcccgag tccgctcgac aatgagttct cgttccattt cagccagggc
    28081 acccatcaca tgaaagaaaa aacgccccat cggcgtgctg gtatcaatag catctgtcag
    28141 gctgcgaaaa ttaacgccgc gttcgcgcag ttcctcaacc agaatgacca gatgccgcat
    28201 actacgcccc agcctgtcca gcttccagac taccagagtg tcacctgctg ataatgtcct
    28261 gagcagtttt ttcagtcccg gcctgtcgga cttcgtgccg ctgattttgt cctcaaaaat
    28321 cagctcacat cctgcgcact ccagcgcatt acgctgcaat tccgtgttct ggtcatttgt
    28381 tgacacgcgt acatagccaa taagcatgag catccccctg aataaaaacc ggagatgatg
    28441 ccagttaccc gttatctctg cattttcata aacgttggtt tgggagaagc ggcaaaacgg
    28501 aatgtgggga caggggaaaa tcagataccg gacatggcct cgtttgccag tggtgatgga
    28561 tggatgaaat tacccaacgg gaaaatcctg caatatggtc gaggtgaggc tatgccgaaa
    28621 ttatcgacgc aaacaatgag gattacattt cctatccctt tccctaaaaa agcggatatc
    28681 gccattctta cccattctgg cgatggcggt gcgccttttg gtgcagggcg tggattcgtt
    28741 atgtccgtag aaggcccaac attaaccggc tttaattctg cttacagaac tgcatcaacc
    28801 agcgacaagg tgtcaatgac ttacagttgg tgggctgttg gtgaataatt ttattcagga
    28861 tgatttatat gaacgaatat gtttatagcg caaggcataa tgcttttttc cctgtggata
    28921 tgattgataa atataaatca gagggatggg atttatcaga tgctaaggag gtgagtcaaa
    28981 atattatcag tgaatttatg gctgaaccgc cacaaggaaa aatccgtatt gccggaggtg
    29041 atgggctgcc cgcgtgggcg gatattcctg cccctacgca tgaagaactt attgaaatta
    29101 ctgaatcaga aagacagcta ctaattaatc aggccaacga ttatatgaac agtaaacaat
    29161 ggcctggtaa agcggctatt ggtcgtctga aaggcgagga actggcgcaa tataatctgt
    29221 ggctggatta tctggacgca ctggaactgg tcgatacttc cggtgcgcca gatattaaat
    29281 ggcctacgcc tccggcagtt caggccagat gacatccggc gcggtgctgg tatctgttgc
    29341 cgtcaccgcg tcagtgtaat ccagcacggc gttaagtcgg gttgtttctg cctgcgtcag
    29401 tttccgtccg gcctgcaatt tcagttgaat cagactgatg gaagccatag cagcatcaat
    29461 cagtgactgg cgctgtgctt ctgccgcttc gactgcggcg ctatgctgtg cctcagtatc
    29521 ggtcacccat ttttcaccat cccatttatc gtatggagat aaaggggcga tagtggttgt
    29581 attttcaggg taatcacccg gagctgtgat ttcttttgat tctcctgttt ctgtgctata
    29641 gacgatttca ccgcgatggt ctggcacata ttcccatgaa tttaaattca cagaacgaca
    29701 gattgcataa ccaaccttat gtatgccagg tgcatcaaga caggaacatg ctggaatacc
    29761 gacccccaca gcaagatatt cagttgatgt ggaaatatat tcccgtgttt cactatcata
    29821 gttatagacg gtaatattcc ccgccttcgt ggcaataaac tcgctattta atacagcatt
    29881 atccattatg cagccctcac gatatagtta aatgcaacat ttcgtgggcg ggtttcgtta
    29941 ccgccaggga gctgaaaagc gttgataatc ccaccgcttg cctgacttat tccatccgtg
    30001 tacaacccgc caccggcact gtcgctgagt gcaaatgttt ttgatggtga actgtttgaa
    30061 gctggtcccc agtattttaa tgcgagcttt ctgacttcat cactctgaat actcaataaa
    30121 gcacgccctg tatcaatgcc gcgcccatca tcccagccac gaataaactc accgcggaga
    30181 tcaggtagtt tccctgatgg atatgctgta gcaagacggg gatatttaac cttatcaaag
    30241 gcagcgccat tgcatttcag ccagcctgtt ggcggcgtag ctgaaggcca cggaacaggg
    30301 acaccaacag gtaatgcaga gccttctccc aaaccaaggt atgcgagaag accagccaca
    30361 tcctttccac tcaaattagt aagcgtattg tccagcggtt gtttgcctgc cagtgcatta
    30421 agcactgttg ttgaaaagtt agggtcattc cccagcgccg ccgccagttc attcagtgta
    30481 tccagtgccg caggggcaga acccaccatt gccgcaattg ctgatttcac aaaagccgtg
    30541 gtggcaatct gtgtattgtt gaccgactgt gccgcagtag gtgctgttgg cgttccggta
    30601 agtgcgggac tcgacagcgg cgctttcagt gccagcgcat tgttaatggt ggtgctgaat
    30661 ttcgggtcat tgttaatggc tgcggcaatt tctttcagtg tgtccagcgt ggctggcgcg
    30721 ccgttaatca gagcggtaat agcagcctgt acaaacgcag tggtcgcaat ccgcgtggtg
    30781 ttatttcctg catcaggcgt cggcgctttt ggttctccgg taaatgtcgg attatgtttc
    30841 tgtgcatact gggtatgagg atcctgtgcg gcaatgtggt ttctcatctg gtcatccaca
    30901 tacagcctta attccaggac ttcatcatcc acgtatttac gggtcgccag taccaccgac
    30961 gggtcgattt tcagcgtgat ggcttcggta ttcgtgacaa ccagaatcat gcggatagtc
    31021 tgggtacgac cactgccttc ctgtaactgc ggtttgtacg tttccgggca gttcgccacc
    31081 gcaatgagta cgccttcatc atcataaagc ccaatctcac ggatccagaa tccgccctcg
    31141 ttctcaggga tgatttgctc cgcaataatc tggctctggt tattcgggtc aacactcaga
    31201 agattcagcg gcgcgatgcg tttctggtta atcagttttg tctgtgccgg gtctggtgtc
    31261 ggcaagacac cattcgcatc accaacggcc atttgcgtca gattcagctt actgccgagc
    31321 atcgtcgcgt tagccagccg tgctgcgccc tgattagtca gaatggcgta gtatttcact
    31381 gtcatgcgtt tactctcagg ttatcaatta aatgaatggc cgaggccggg aaataatccc
    31441 ctccgacaat aatggcctcc ggggtgtagg gataaaccgt cagggcgtca ccgtgatagc
    31501 atcccgcacc ggcaaaaatg ttgccggttg tacttaaact gatagccagt cccgtcagat
    31561 ggcggcttgc cggttttgca tcagcaacga gacgctccag ctcctgatac atttcttcgg
    31621 taataccctg ctcaagcacg ccaacaacga tacggaacgt ccccggctcc tcgttgagct
    31681 gccaccactc cctcacctca atcagatagc cgagcggctc caccacacgg cgaatcgcac
    31741 caatagtgcc cttatgacag tggatgaaat acgcatcgcg gataacagcg cgttttgtcg
    31801 cttccggcca attatcatcc cagcggtcaa ccgaaaatga ccacgccagc cacggcagca
    31861 gatttgccgg gcaggtgtcc gggttccaca gctcacgaat actgaccggc gttttttcaa
    31921 tttccgcaca ggcttttgcg gcggcaactt caaacggtga tgagccggtc ggcagcagtc
    31981 gcgaatcact catccgagcc tccggtcaca acgctgtatt cggtacagaa agacgcctgc
    32041 gtattgttga gcacgatgtc ggccagtggt gcagccagtt cgacacgctg cacgccttcc
    32101 acatgcaaag cggcataaat ggcagacaga cggatgtcgc gccccagccg gtgctgtgcc
    32161 gtgatgtacg cttccagttt tttcacggca gcagcgcgga tgggttcgct ttcgggacca
    32221 gggtaaaggt aaagcgtggc gtttatctgg tattcaacga tggcggcaga ctgcacggtc
    32281 acgcggtcgg ccaccggcct gacgtcctcg ccattaaggg cgttacgcac cgccgccagc
    32341 aggtcttcgg atgccacacc gttattttcg cgagacagca cggagatggt gacgcaggcc
    32401 ggagaagggc tggtgacaga gatatctgcg acacgcccgt cggcactgcg accatgatac
    32461 tgataggcac ccaccgaccc ggcgacgctt aaaccttcaa acgcctgctg aatacgcaga
    32521 cgataatcgg tgtcagattc catcactgcc ggtgtcggcg ggatggtcga atcatctgcc
    32581 ggggtgatag tcaggcgcgt ggtgttgtaa ttggcaccaa tcacatcaag gtcattaccg
    32641 gcggcacagg ccagcatcac cgcccgtgcg gcctcattca cacgctgacg ccagataagc
    32701 tcacgataag cattttcctc cagcagtttg acgagaggct cagattccag cgtcagggta
    32761 cgggcgaccg cctcctgctg gtcttccggg taaagggaaa tcagtgtcgc cttgcgttcg
    32821 gcaagaatgg tttcaaagtc cagctcctcg accacatccg gtgcgggtag ctggttcagg
    32881 tcgataatcg gcatggtttc aactcacagg gatggttaac gaaagtggct ggccggtgtc
    32941 gttgtgctgg ccggttaatg taactgtcat tcgcccgtca aagctgcgcg ccgtggtgac
    33001 ggatgacagg gtgacgcggg gttcccattt cagcaccgcc atgtaacagg cgaccttaat
    33061 ctgcaactca agtgccgggg tctgcggctg gtcaatcatt gacgccagca acgagccgta
    33121 atcacgacgc atcacccgtg agccgaccgg tgtgcgcagg atatcgccga tactctggct
    33181 gatatgctca aggtcagtga cagtctggcc atcactgcga ttcattccga gataacgcgc
    33241 tgtcatagag gactcccggt tgtgccgccg ctgtcgccgg ggtgtttatg ggtatgcagt
    33301 accttaccgt ttgatgagag ttcaccgccg gtgtgttcaa tgttgccgcg catcgtcccg
    33361 cccttctgca cttccagcgt gccggtaatc agcttgtttg tgcagaccac ctccggtgtg
    33421 tccagggtga cgcgggttga tgctttcacc atgaccaccg gcaccgtggc agtaacagaa
    33481 tcagaagccg tcacgctggc cgttttaatt ccgcttgccg tgagtgcact ggtttcaggt
    33541 tcatactcaa tcaccgcccc gtcaggaaaa cggatatgca gggcatccgc cgacgcagac
    33601 ggcgcggggt tatcaccgga ataaatcccc ggcagaacga acgccgtgtc gagttcaccg
    33661 cccacggcca gaatcagcac ctgttccccc acggaaggtg cccaccatgt gcgcgaacga
    33721 ccggcgcgat gggtcagcca ctgcagccag tcggtacaca tgccgccggt ctgcacacgg
    33781 cagcgaccgg cgttaaggtc ggtttcgacg ataacgccgg tgcgaatcat gttgcgcagt
    33841 gcgcgcgcga gttcctgaat atttgcgaga gtgttcataa cgggaaggat gccgccgggt
    33901 cataccggcg gcaatgtgac gatgaggtgt cgggaatggc acaactaacg gtcgaggtgc
    33961 gccagaataa tctcttcaat catctgcaca tcctcaccgg tgaagccgag cagagggcgc
    34021 gccggataat caattttctt accgtctttc cggttttctt ccgacagacc aaactgatgc
    34081 acactggcga ttttcggtga cttcccgccg taaaattcca ttgctgcctg ttccgggctg
    34141 gcgcggatat gcaaaaaacg actggtgata agtttcgcaa acatttttcg cttaacgcga
    34201 ccggtctttt ttctggcgct ctgctgctgg cgtggcgcgt agggtgtgcc gtccggggct
    34261 ttctgtgcca tcacccgacg ctgctgactc tgccgcagac gtttcgccag ttcggcactc
    34321 agtcgccgac gccctgacgg tgacagcgat tcaatcagtc cggtcagccg gtcttcaaaa
    34381 cgcttaaact cattcatccc acttgctcac cagttcgcca ttgatataaa gctccatcgg
    34441 gcgggtgacc ggctccggcg gcgtgggttc cgggatattc ttcacatgca gcgcgccgtc
    34501 cacctcactg accagcgtgc gctcggtcag catcaggctg atgctgatat caaagctgct
    34561 gtcattgttg atgtctgcat aaaacgtgaa accttttttc tggccttcgt cggtggtcat
    34621 gatgtcgggc tgattttccc gcagccacgc cagcaccggc acgatgagca ggtcaaaatc
    34681 accggtaaag tcggtcacaa tgacattgag cgtgtaacgt ttttcgaatg acaacgacgc
    34741 cgccagcgtg gaggcaatac tcccgttatc cacgaatatc cgaagcatct ctggactggt
    34801 tttcagcacc gtgacggcat cagtcagcgc cctgcgcagg ctgtcgggtt tgagcatcgt
    34861 tttcgtcctg acagtgttta atcattttta cctggctggc acagcgtgcc agcgcgttct
    34921 caagctgccg gatatcggca cttaaatcgc cgttcgtctg cgggtcactg cccggcatcg
    34981 ggcaaaggct cactttcggg caggcgttgg cgacaatcac tggcgtctgc gcaggcgggg
    35041 cgctggtgca accggcgcac agcatcaggc aggtcagcgc cgtaccagcg gcgaaaatct
    35101 tcgttttcat taagtaacct cgtgatggtt ttctcgcgct gtgcttcacg cttcgcggcg
    35161 ttctccagtt cctgacgcag tgccacctgc gccagctcgt ttttgtctgc cctggtgagg
    35221 gcaacatgaa gctgattttt cagcatggtg atggtcgcct gctgttcact ggcgacgttg
    35281 ttcgccctgt ccagcgaggc gcgcaggctg gcatttttgt gtttcaccag aaacagaccg
    35341 gccaccgcca gcgataacaa cacgaccagc acaatcatca gctttgacat ggttcccgcc
    35401 cctcaaaacg ctgacggcag gccgtacgta tcagccggaa gaacaccgat gccacgagat
    35461 aaatcagcgc ggtaaaaatc cacccggcag caaccagcga gataaacgtc gccaccatca
    35521 ctaccagagc cactgaccgc ctgcgccacg gcaccggctg caaaaacagc gccgtgacaa
    35581 ttttcacggc cagcgattcc ggcggcagct cccgcccgta gcgttccagc acatactcag
    35641 tggcatacac cccgacacca ccggcaacca cacagataac cgtcgccaga atcgcccagg
    35701 cagcgacaaa actgacggcc acgctctgcg gataaatcag ggacagtgcc agcatcagcg
    35761 ccagcgacac gttcagcatc agtgaaaggg ataatttctt catggtgttt actccgttta
    35821 agccggtacg ccgccagcgg tacgccagac ggtgaccagt ttttccagtg aatgctcacg
    35881 ctgaccgtaa ccggcacccg gcagggacgc ccagatattg cgacagcgtg aaatggcgcg
    35941 ctcaatgcgt cccgcccgga tgtcatccag tgcaccgcgt tcgcggatca actgaatggc
    36001 gagtctgtcc tgtgacaacg gactgaaatc cggcagggca agctgtttgc ggtaatgcgg
    36061 ccagaacagg taaagctgct gatagcgacc ggaggccgtg gatttttcac cgcgacggtt
    36121 aaacaccttc gccggtcgac catgtgcgaa cgggtggtca ctgtagtcgg tgaaaatttc
    36181 ctgctttccg tccagtccgg tgactatcac gtcatagccc cggtttttcg tcagcggatg
    36241 gttcgccgtc ccttcggaca cggccagcat gtcgagaaag gcggcgatat tctgatgcgt
    36301 gttaattacc ggcattacgg tttccccctg ctcttaaagc ggcgctgaat ggcaatctca
    36361 atcacctgat aaccggcgat acccagcatg gagccgatgc cgcacaccgc aggcagtgac
    36421 aggtcaggaa actgcaccag aacaacaccg gcaaccatcg agacaaaacc accgagcaac
    36481 atgcgcccga taaacagacg cggggtgatg ggttcaccac cggcaagcac cttgccgaca
    36541 acaatcagca ccccaatcat gaaaagcgac aggacgcttt tttcttctgc tgtcatgcgt
    36601 tactcccaca gattgacagt ttcagccacg ggcgcggtct gaacgtcggg cagttcgacg
    36661 gcggtgccat gtggcagcac cgcccccagt tcagccagtc ccggatttgc ggcgagcacg
    36721 gcttcgacca cgccctcagt gcgcccgtaa taccggacac aaatggcgtc gagcgtgtcg
    36781 ccctgtagcg caaaggtctt catcagattt gactcacgat gcagcgcggc ttgtcctgga
    36841 tacgcgccac cgcccagcgc atatcccgcc acagctcatc aatggtgctg tcaatgctgt
    36901 cggccttctt gtcgcctttc gcactggcat ccacgccgcg ataacgctca taaagcgatg
    36961 cggtcgccat cgcacacacg gcgcgctcgt agtaaaaaac tttgatgctt tcaccgtcga
    37021 tgtcgtccgc cgggacgtcc gccagacgcg taaaaccggc ggcaattttc tgttcgcggt
    37081 actcgtacag ctccgcattc gtttcagcca tgcctgactt gatggcctca cgcagacggg
    37141 cgggggcgac ggtctgctca aggcgcatac gttcccggac gcgcttcggg tcgatatcgg
    37201 gaaaaaagaa cgtgttttta atcaccggct cgtcgcctgc cggttgcggg atgaccaccg
    37261 taccctcacc ggacacggga gcctcctttc gcggaataat cagcgtcatc atgactacct
    37321 ctgaaaagtc gggcggtgga cgccggtgca gtgtcaggtg attcaccctc actgaccggc
    37381 gtgccgccct ggcgcggggc gcattcggtt gttaactggc tttctttttc gggcgtccac
    37441 gttttgccgg tgtcacgctc cgggtcttac gcggggcacg ggtggccgct tttggctgcg
    37501 gctccggctt cggtttcagc tcccgctcca gtcgttcaat ctcttttttg acgcctgcct
    37561 gacagtcgag ctgtgtcgca cgttgcaggt gagccagcgc accggcggca tcaccagcgt
    37621 cacgcagaaa cagaccggtg attttgtgca gctttgcgcg cacttcatca ggcatgtcag
    37681 ccgtggcggt cagttcaagg gtgtccgtca gcaggcgggt atccacagac tcaccggcag
    37741 cgtgggcacg catggccgca agcgcgacct cctcggtgaa catgtacggc ggggtgcggc
    37801 ggtgttttcc cggcatggtc agaccgtact tcagggcata acgggcaatc tccagcgcac
    37861 cggcaatatc gccggtatcc agacgccaca gcatgaccgt catcagaatg tcatcctgtg
    37921 cacctttgcc ctgctccaga acgccgttca cccacggcaa ccagaacggc agcagttcgc
    37981 gttttttcgc ggccttcagc tcttttgaat aaatcgcttt cagtgtgcgc tggtctgcgg
    38041 cgagcttaac cagcatctgc tcatagacag ttgcatgtcg cagcggggcg gcttcccgct
    38101 gcgcggtcat cgctgccgag acccgcatca tgtggcgctg tgcgggactc gtcatcggtt
    38161 acgctcccgg ctctgcggtc gccttagccg gtgtggagaa atcaccgacc ttaatttttt
    38221 ccaccagaca accggcggcg tagtcttcca ccacgtaatc aatgttcatt gactcgtagt
    38281 tctccacgcg gtcgagtttc gggttttcct caatcacgcg gcgatggctg tcatccatgt
    38341 agtagatgga caggttttcc agcttcgtga tgagcatcgc atccgccggg aagtacggga
    38401 cgcgtaccgc tggcaggtta ccgatgcgtt tctggctgat gatgacgtca gcggccagca
    38461 tttcgctgtt gtcctgctcc ttgttaacga tggggaaata cttgtccgcc agtagctgac
    38521 gccccacaat caccacaagg tcagggtctt cctgatacca cggctcaatc aggttgttgg
    38581 tcgcatccat caccagtgcg tcgaggctgg cataatcacc gcccttaccc acgcggatga
    38641 cctcagaggt ggtgcgacct tcctcgtcag tgaccttgct catcacgcgc gccggggctt
    38701 cattgcggta tttctgcagc cagccgaccg ccacatcctg caacatcgga ttgctgctgc
    38761 ggtcagaggt ttcggcacgc ttcacgccgt taaaaccggc catgatgaaa tcaagggact
    38821 ggcgtttgat aatggcgtta cggacacgga gctggaaatc ctgataacgc gcccacaggt
    38881 caagcgtttt gtagcggata taaaaatcga agttaatctg gtcgcattcg tacttgtttg
    38941 acgccagctt cgagaagtcc ttcggctgac gctcggtgcc accggcggtg tcggtggtgc
    39001 tggcgatgga gccggtgaca ccaataccaa ttttttcccc tttcatttcg ctgaccggca
    39061 caatgttgat gcgggtcaga aagtcagagg actcctgcat ggtgttcatc agggtctggg
    39121 tgaccgaagg ttcaacggtg aattttttcg acacatcacc ggcgtcgatg ccgttcagtt
    39181 cggcaacacg ggacaggtag gcattaaatt taaagcgggt ttcctggcgc atagttattc
    39241 ctgaaattaa gggttaatcg tgaaggtttt cccggactga cgccggtcag cagttcgtca
    39301 tcagggcgtc accgccaccg ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    39361 tgtggtcgag actgtttttc aggcaggtga atgcctggct ggtttcatcc gccctgtcag
    39421 tcacatcctg cttaagtgcg gaaaaggcgt tttccatctc agcgaggcgc tgctcagtgg
    39481 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    39541 gacgggcgtc atcgctggcc tgtttgcggc caaaaatgga cttcaccttt tcggtcaggg
    39601 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    39661 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    39721 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    39781 aacatttgcc ggtattggca aagttcggct gaatttccat tgaggtatag accttctgcg
    39841 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    39901 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcgacc acatcgccat
    39961 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    40021 catagactcg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    40081 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    40141 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    40201 tgcgcatcct ccgccatcag tcccggatgg cttatcactg acacaacagc accttagcga
    40261 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    40321 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    40381 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacagtgcag
    40441 agctggaaac agcgcgacgg ctgggacagc gttgccccca tcagccgtgt cgaaatgagt
    40501 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    40561 gaaattgacc tgctgggacg ccagattgaa cgactggcac gggtaaaccg ttacagccag
    40621 accggcaacg aggcagacct taatccgaac gtcgctaacc gcaacaaagg cgggcgtcgc
    40681 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    40741 gagcagtctt tcgactatca gttgcactgg tatcgcgccg ggcttgagca ccgcatccgc
    40801 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    40861 cgtgccctga aaaccggtca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    40921 gtgttccgcg aatacatcat cgcctttgcc cggctggttg acgttgacct gaccggtgac
    40981 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    41041 gcacaaagcc ataacggcga cctgtacgtc gacgagattt tctggatacc gaattttcag
    41101 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    41161 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtccggtga actgtttaac
    41221 cggggacgcg ccagcgccgc tgaacgcgtg gaaatcgacg tcagtcataa cgcccttgcc
    41281 ggtgggcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgcgctg
    41341 aaaggcggct gcacgctgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    41401 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg cgtcggtgtt cccgttcgag
    41461 gagctgcaac gctgcatggt cgacacactg gaagaatggg aagactatgc accctttgcc
    41521 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    41581 agcgccggat gcgtggtact ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    41641 gagcgtcacc agtggaaagg catggacttt gccactcagg cggaatccat ccgcaaactc
    41701 accgaaaaat acaacgtcga atacatcgga attgatgcca ccggcctcgg tgtcggcgtg
    41761 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    41821 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    41881 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    41941 ggacgcagcg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    42001 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    42061 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    42121 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    42181 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    42241 ggtatgagcc accggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    42301 acagctcacc gatttacgtc aaacgtaata ttctggcctc gacatttatc ccgcatccgt
    42361 ggctttccca gcaggatttc agccgctttg tgctggattt tctggtcttc ggtaatgcgt
    42421 ttctggaaaa gcgatacagc accaccggta aggtcatcag actggaaacc tcaccggcaa
    42481 aatatacccg ccgtggtgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    42541 cgacagcctt cgcgaccggc tccgtgtttc acctgctgga gccggatatt aatcaggagc
    42601 tgtacggcct gccggaatat ctcagcgccc ttaactctgc ctggctgaat gagtcggcca
    42661 cgctgttccg ccgcaaatat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    42721 ccgacgcatt gcaggatcgc gattacattg actcaatccg cgaaaacatg gctaattcga
    42781 aaggccgcaa taactttaaa aatctgtttc tctatgcacc gcagggaaag ccagacggca
    42841 tcaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    42901 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg ggcggcaagc
    42961 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    43021 agcttatccc gttacaggac aggattcggg aaataaacgg ctggctcggc caggaggtca
    43081 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    43141 gcttttttac accccgtcat cacgccctca cacgctcacc accgcacaaa acaccccgca
    43201 gacacaccaa cgccccggcg cacactctaa acgccatcac gacgcgctca gacgctgaaa
    43261 aaataaaatc agcaccaccg ccagcgcgca gtgctttccc cgcctcgccc gcccgcttca
    43321 tggggcggtt ttaatgcaga tgcacgaaca cgccgaaggc gcgccagtac cggcggcgct
    43381 cacacgtgac tgggaaggaa aacgcatgcg attcgatgca gtaatgcatg cagagctaaa
    43441 aatggcagaa catactgaaa aaacgcataa aaaaaccggc attcaggttg ccggtttgag
    43501 tcgacttcat tgtggttact ggccgcgcaa tgcgccaata atactggtaa ggcaacaagt
    43561 ggcaacaatc agcaaaaaaa ccgtcgtcca cggattttca taaatgagag ataacatatt
    43621 gataaagatc cttttatttg ttcattctga tgtggtttaa aaggtgtttg ataacgaccg
    43681 taagctcatc tttactggct tgctcgacca ttttttcggt gtagctttcc acttcgcggg
    43741 aactcaggtc gttatttgag gccattacag tgagtctctt tgcccagtcg gcataagggt
    43801 ctttaattga tgaaagggaa ttatgcatgt ccaaaaacct cgaattattt aaccagcaga
    43861 cggctgaaat ctttgcggtg ctatgggata actttcctgt accacaagtc atcacctaca
    43921 aaaaatttaa cgccgcgtta cctgatgact actttgacca acttaactca ccagaaatga
    43981 aagcactgaa tcagttgcgt agtgtggttg atggcacatt cactttccta agcgaaaacg
    44041 gctatattct gtacggaaca gaccatcaga ccggttttca tgatgtgcgc ctgaccgaga
    44101 aagcactcgc ggtgctcaac aaaaaacccg aagcacttgg cggtaatgaa acgatgggag
    44161 ataaaattat cagtgcagta aaagacggga ctccaggtgt cattgctgga gcagtgacaa
    44221 acctgttgac tcttggtgtt aattttgtga cgtcagcttc atagctaact actttctgag
    44281 gtttctaatc aacgccgcct gcaccgaaag taaattcctg cactggcggc gttactaacg
    44341 ccaaagttat tttaattatt gatgctatta tgcaatacat tcaatatgtt aatttgcagt
    44401 attagccttt aaagtaagca ccttagattt gtaacgttgc tcaatcactc ccattgaatg
    44461 caaacgacca aagagctttt tggcaatgat tgtttctctc gcattttcgg cgtctttaat
    44521 tattttccaa taatctattt ccctagccat caaaaaatgc tgctcattcc taacttttaa
    44581 tgcattgcct aatacaataa ctccagttac caacttaggc ttatctctgg catatagttt
    44641 tgttttcgtt gggtgtataa catgcccatg cttattaacg atcttacgaa ttacagcgca
    44701 gaacaatcga tttacgttac tacctgaaaa agtgagatca tcaacataaa cagtcatgtt
    44761 aactcgtaac ttttggcaaa gttgatatat ttcaccaaac attcttgaat ttgcaaaata
    44821 tgccaacggc atactgatgc gactacctgt tggcaatcgg tcatgacaag tacatatgtg
    44881 agacaaaaca tcagcaacat cagatgacat tttcatcacc gaaaagaaaa aaga
//
LOCUS       DAZFAH010000017        42704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFAH010000017 DAZFAH010000000
VERSION     DAZFAH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.017"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1396)
                     /locus_tag="ACHJWB_000889"
     CDS             complement(<1..1396)
                     /locus_tag="ACHJWB_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFO4312569.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYVPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1801..3450
                     /locus_tag="ACHJWB_000890"
     CDS             1801..3450
                     /locus_tag="ACHJWB_000890"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4312570.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3501..4103)
                     /locus_tag="ACHJWB_000891"
     CDS             complement(3501..4103)
                     /locus_tag="ACHJWB_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4312571.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4622..5521
                     /locus_tag="ACHJWB_000892"
     CDS             4622..5521
                     /locus_tag="ACHJWB_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4312572.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDTPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5570..6583
                     /gene="yhjD"
                     /locus_tag="ACHJWB_000893"
     CDS             5570..6583
                     /gene="yhjD"
                     /locus_tag="ACHJWB_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4312573.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            6994..8316
                     /locus_tag="ACHJWB_000894"
     CDS             6994..8316
                     /locus_tag="ACHJWB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312574.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8550..10610)
                     /locus_tag="ACHJWB_000895"
     CDS             complement(8550..10610)
                     /locus_tag="ACHJWB_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4312575.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10680..11447)
                     /gene="pdeH"
                     /locus_tag="ACHJWB_000896"
     CDS             complement(10680..11447)
                     /gene="pdeH"
                     /locus_tag="ACHJWB_000896"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4312576.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11679..12608
                     /gene="kdgK"
                     /locus_tag="ACHJWB_000897"
     CDS             11679..12608
                     /gene="kdgK"
                     /locus_tag="ACHJWB_000897"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4312577.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12704..14200)
                     /locus_tag="ACHJWB_000898"
     CDS             complement(12704..14200)
                     /locus_tag="ACHJWB_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4312578.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLTESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINRAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14421..15707)
                     /gene="dctA"
                     /locus_tag="ACHJWB_000899"
     CDS             complement(14421..15707)
                     /gene="dctA"
                     /locus_tag="ACHJWB_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4312579.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15890..17878)
                     /gene="hmsP"
                     /locus_tag="ACHJWB_000900"
     CDS             complement(15890..17878)
                     /gene="hmsP"
                     /locus_tag="ACHJWB_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4312580.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17960..21427)
                     /gene="bcsC"
                     /locus_tag="ACHJWB_000901"
     CDS             complement(17960..21427)
                     /gene="bcsC"
                     /locus_tag="ACHJWB_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO4312581.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQL
                     GDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVASG
                     VTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGTGG
                     YSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDCSG
                     NRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAHRR
                     PISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTGKN
                     VEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEYLS
                     FAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDGGS
                     SQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDLPP
                     QPLIPYADW"
     gene            complement(21409..22515)
                     /gene="bcsZ"
                     /locus_tag="ACHJWB_000902"
     CDS             complement(21409..22515)
                     /gene="bcsZ"
                     /locus_tag="ACHJWB_000902"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4312582.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22522..24861)
                     /gene="bcsB"
                     /locus_tag="ACHJWB_000903"
     CDS             complement(22522..24861)
                     /gene="bcsB"
                     /locus_tag="ACHJWB_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4312583.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24872..27490)
                     /gene="bcsA"
                     /locus_tag="ACHJWB_000904"
     CDS             complement(24872..27490)
                     /gene="bcsA"
                     /locus_tag="ACHJWB_000904"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4312584.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27487..28239)
                     /gene="bcsQ"
                     /locus_tag="ACHJWB_000905"
     CDS             complement(27487..28239)
                     /gene="bcsQ"
                     /locus_tag="ACHJWB_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO4312585.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28251..28439)
                     /gene="bcsR"
                     /locus_tag="ACHJWB_000906"
     CDS             complement(28251..28439)
                     /gene="bcsR"
                     /locus_tag="ACHJWB_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4312586.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28712..30283
                     /gene="bcsE"
                     /locus_tag="ACHJWB_000907"
     CDS             28712..30283
                     /gene="bcsE"
                     /locus_tag="ACHJWB_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4312587.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLHYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30280..30471
                     /gene="bcsF"
                     /locus_tag="ACHJWB_000908"
     CDS             30280..30471
                     /gene="bcsF"
                     /locus_tag="ACHJWB_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4312588.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30468..32147
                     /gene="bcsG"
                     /locus_tag="ACHJWB_000909"
     CDS             30468..32147
                     /gene="bcsG"
                     /locus_tag="ACHJWB_000909"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4312589.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32234..32341)
                     /locus_tag="ACHJWB_000910"
     CDS             complement(32234..32341)
                     /locus_tag="ACHJWB_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4312590.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32717..32824)
                     /locus_tag="ACHJWB_000911"
     CDS             complement(32717..32824)
                     /locus_tag="ACHJWB_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4312591.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            33300..34571
                     /locus_tag="ACHJWB_000912"
     CDS             33300..34571
                     /locus_tag="ACHJWB_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4312592.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(34601..35605)
                     /gene="dppF"
                     /locus_tag="ACHJWB_000913"
     CDS             complement(34601..35605)
                     /gene="dppF"
                     /locus_tag="ACHJWB_000913"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4312593.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(35602..36585)
                     /gene="dppD"
                     /locus_tag="ACHJWB_000914"
     CDS             complement(35602..36585)
                     /gene="dppD"
                     /locus_tag="ACHJWB_000914"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4312594.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(36596..37498)
                     /gene="dppC"
                     /locus_tag="ACHJWB_000915"
     CDS             complement(36596..37498)
                     /gene="dppC"
                     /locus_tag="ACHJWB_000915"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4312595.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(37508..38527)
                     /gene="dppB"
                     /locus_tag="ACHJWB_000916"
     CDS             complement(37508..38527)
                     /gene="dppB"
                     /locus_tag="ACHJWB_000916"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4312596.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(38835..40442)
                     /gene="dppA"
                     /locus_tag="ACHJWB_000917"
     CDS             complement(38835..40442)
                     /gene="dppA"
                     /locus_tag="ACHJWB_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4312597.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            41223..41831
                     /locus_tag="ACHJWB_000918"
     CDS             41223..41831
                     /locus_tag="ACHJWB_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4312598.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            41976..42704
                     /locus_tag="ACHJWB_000919"
                     /pseudo
     CDS             41976..42704
                     /locus_tag="ACHJWB_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT        10031 a        11665 c        10758 g        10250 t
ORIGIN      
        1 attgtttatc ttgcatatac ggcgtgtaca ccccgttcag actgtgcagg aaagctacga
       61 tatcatccac atcctcctgc ggcagctctt tgcctacctg atagcgcagc atcagtttca
      121 ccgccccgtc cagcgtcggc acgtcaccgc gatggaagta cggtgccgtt aaagcaacgt
      181 tacgtaaacc gggtactttc tgacgcaatt tatcacgctc ttctttagtc acattcatac
      241 gaccaatatc cgccgccgta atttccccaa agttaaagtc ttttttcagc cccaacggtt
      301 caaaggaacg tccgccgaga ataataccac catgacaagt tgcacattta ttatctttaa
      361 ataattgata gccttttttc tgttgcgccg tcagcgcatt ttcatctcca cgcaaccatt
      421 tatcaaatgg ggaatccggc gtaattaatg ttttctcaaa ttcagcaatg gcatcagtaa
      481 tattttcgcc actgaaacct tgcggataaa cttcgaggaa ctgcgcttta agctgcggat
      541 ctttttccag cttagcaata atttcgtccc aggatttcga cgccatttca atcgggttca
      601 acggcggtcc acctgcctga tcctgcaatg ttgccgcacg accatcccag aactgctcaa
      661 cgttaaatac tgagttaaat accgtcggcg cgttaatcgg cccaactgcg ccaccaacac
      721 caatcgatgt ttttctgcca tcgacgcccc ccgcattcaa cgcatggcaa tgagcgcatg
      781 aaatggtgct atcagccgat aaacggggat cgtgatacag cgcaaagccc aacgccactt
      841 tttgcgcatc ggtaggcagt ttttgcggga tgggctgcac cggttcattg cgatgctccg
      901 gagcagtatc attgctggcg taatattccg cgcgctgttt tgcaatccag gccagtattt
      961 ccgcccgctc ttcatcactc accttacccg cccagtgtag cgcggtataa cgcgttggtg
     1021 gcatagtttc atactgcatc acccattcaa tcttattcag atcgctttgc gaaacgggtt
     1081 tatcagccag cagtgccgca cgcacggcct cgaggttaaa agatttatat ccaagcttaa
     1141 tgtcgtaatc catcaactgt ttcgcgccag gaacataata ataggcgggt aattctgccg
     1201 aaggcgtgtg gcaatagtcg catccttttt cgcggagaaa gcctaaaacc ttattatttt
     1261 cactgacagc agatgcctga acatcggcct gtttactgcg tttattatcg tggtaccaca
     1321 cataaccaga taaccctaaa tagcaaatcg cgacgccagc caggccgatc gcggtaatac
     1381 gtgagaccat tttcattatt cttccctcac ggtggtgagt tattgtgacc cagaaaattt
     1441 tccagaatgc tgcaaacgtc tctgcgagac aaagccatca taaaaaacag ggagatacgc
     1501 tttttgatag gaatcaatca attacatgaa tataaactat caataagata gcctgtgcct
     1561 attacccaga tcaggaatgg catatcatga ttgcggcgag agcaggattg aatgttctga
     1621 aaatgaaaaa caacacgaaa tccgtatttt tataaattcc atggatatta gtgcgttata
     1681 ttttttgaca ggttaataaa cgtgatccac cgcacgcttt gtcgcccacc aggcggagcg
     1741 aatgactacc cttaaagaaa agcccgataa ttagcgacga atttcggagg ttggatcctt
     1801 atgctcaatc agaaaattca aaaccctaat ccagacgaac tgatgatcga agtcgatctc
     1861 tgctatgagc tggacccgta tgaattaaaa ctggatgaga tgatcgaggc agaaccggaa
     1921 cccgagatga ttgaagggct gcccgcctct gatgcgctga cgcctgccga tcgctatctc
     1981 gaactgttcg agcatgttca gtcggcgaaa attttccccg acagtaaaac ctttcccgac
     2041 tgcgcaccca aaatggaccc gctggatatt ttaatccgct accgtaaagt gcgccgtcat
     2101 cgtgattttg acttgcgcaa gtttgttgaa aatcacttct ggctgccgga ggtctactcc
     2161 agcgagtatg tatcggaccc gcaaaattcc ctgaaagagc atatcgacca gctgtggccg
     2221 gtgctaaccc gcgaaccaca ggatcacatt ccgtggtctt ctctgctggc gctaccgcag
     2281 tcatatattg tcccgggcgg ccgttttagc gaaacctact attgggattc ctatttcacc
     2341 atgctggggc tggcggaaag tggtcgggaa gatttgctga aatgcatggc cgataacttc
     2401 gcctggatga tcgaaaacta cggtcacatc cccaacggca accgcaccta ttatttgagc
     2461 cgctcgcaac caccggtttt tgcgctgatg gtggagttgt ttgaagaaga tggtgtacgc
     2521 ggtgcgcgcc gctatctcga ccaccttaaa atggaatatg ccttctggat ggacggtgca
     2581 gaatcgttga tccctaatca ggcctatcgc catgttgtgc ggatgccgga cggatcgctg
     2641 ctcaaccgtt actgggacga tcgcgacacg ccgcgtgacg aatcctggct tgaggacgtt
     2701 gaaaccgcga aacattctgg tcgcccgccc aacgaggtat accgcgattt acgcgcgggg
     2761 gcggcctccg gttgggatta ctcttcccgt tggctgcgtg atactggtcg tctggcgagc
     2821 attcgtacca cccagttcat ccccatcgat ctgaatgcct tcctgtttaa actggagagc
     2881 gccatcgcca acatctcggc tctgaaaggt gagaaagaga cagaagcact gttccgccag
     2941 aaggccagtg cccgtcgcga tgcggtaaac cgttacctct gggatgatga aaacggcatc
     3001 taccgcgatt acgactggcg acgcgaacaa ctggcgctgt tttccgctgc cgccattgtg
     3061 ccactctatg tcggtatggc gaaccatgaa caggccgatc gtctggcaaa cgccgtgcgc
     3121 agtcggttac tgacacctgg cgggattctg gcaagcgagt acgaaaccgg tgaacagtgg
     3181 gataaaccca acggctgggc accgttacag tggatggcaa ttcagggatt taaaatgtat
     3241 ggcgatgacc ttctgggtga tgaaatcgcg cgaagctggc tgaagacggt gaatcagttc
     3301 tatctggaac agcacaaact gatcgaaaaa taccatattg ccgatggtgt tccccgcgaa
     3361 ggcggcggtg gcgagtatcc gttgcaggat gggtttggct ggactaacgg tgtggtacgc
     3421 cgtttaattg gtttgtacgg cgaaccataa tatttttaca gccagccgct aacttcctgc
     3481 tggccgtaaa attatcctct tcaggaggag atatttaaca tcattgccgc ctgggtgcga
     3541 tttttcactt ccagacggcg atacagggat tccagatgcg cttttaccgt tccggtactg
     3601 atattcagcg ctctgccgat ctccttattt gattcgcccg ccgctaacat ggttaaaatc
     3661 tcccgctggc gggcgcttaa cgatttgaga tctttaatgt ccttttccgg cgtcgtccgc
     3721 cagtctccag gcagaaacat catccccatc gccgcactat ttaccgccaa cgcaaatgtc
     3781 tcgacggttg aatcacgagg cacaatggcc agcacattaa aatgaataac ttcctgtaac
     3841 caccgtttat tgcaatccgt cgccgtgatt aacaccttaa cctcaggaaa ctgcaccacg
     3901 gttttttgca gcaaccagta gcaaaactca ccatcctgat cgccatcgag cataactaag
     3961 gcttcagggt aactttccag cttttgccat aactcgtctg cctgactggc cccctgaata
     4021 ctcactcctg gaatacgctg ctgtaaactg attttcattc catgaataaa tattgactgc
     4081 ctgtcaaaca tgactatttg cataactgaa tctccacctg aatacgttaa aaagacttaa
     4141 gtagtggaag ggtattaccc gcgagaaaaa ataagaattc gccatttggc ggtggccatt
     4201 ctacagagat gacgcgtaga aaatagttac cgatataaat agttacagct aaacgcctga
     4261 aattacatgt cgagggcact atttaaaaca attttgagga tttccttata ttgatggtta
     4321 gtacgcatgc aattaaaaat gaaattccgc gaccacaagc caaaataaca aacggcaagg
     4381 agacaaaaat aagcacaaat agccaataca tcctctattc actttaaagg gaatcgctga
     4441 aaaatacgct ctgtttaagg ggattcacct tctcataaag ctattccgcc cttttcctgc
     4501 tgagaaatcg ccacattcgt cataacaaca ttgtgaaacc cggcattaga tgttagaaaa
     4561 aacaataaac aatgcgatat gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga
     4621 aatggataaa attcacgcaa tgcagttgtt catcaaagtc gcggagctgg aaagtttttc
     4681 ccgcgcggcg gatttctttg ctttgccaaa aggaagtgtt tcgcgccaga tacaggcact
     4741 ggaacatcaa cttggcaccc agcttctcca gcgcaccacg cgacgggtca aactcacgcc
     4801 agaaggcatg acctattatc aacgagcaaa agatgtgttg agtaatctca acgaactgga
     4861 cggtctgttt cagcaggatg ccaccagtat cagcggtaaa ttacgcatcg acaccccgcc
     4921 aggaatcgcg aaaagcctgt tactgccgcg cctgtcggaa tttctctatc tgcatccggg
     4981 gattgagctg gaactgagta gccatgaccg tccggtagat attcttcatg atggttttga
     5041 ttgcgtgata cgcactggcg cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa
     5101 actgaccatg gtcaactgtg ccagtccgca ctatctgacg cgctttggtt atccgcaaag
     5161 ccccgatgat ctgacttcac acgcaatagt gcgttataca ccgcacctgg gtgtacatcc
     5221 gttaggtttt gaggttgcca gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt
     5281 gacggtaaac agtagcgaaa actatctcgc cgccggtctt gccggtctgg ggattattca
     5341 gatcccgcgc attgccgtgc gcgaagccct gcgtgccggg cgccttattg aagtgttgcc
     5401 aggctaccgt gccgagccgc tctccctttc gctggtttat ccgcagcgtc gggagctttc
     5461 ccggcgtgta aacctgttta tgcagtggct ggctggcgta atgaaagagt acctggactg
     5521 accgactata ctttttaaga atgaccacga cagaaggaca aaagagcgga tgacgcagga
     5581 aaacgagatc aaacgtccca cccaggatct ggagcacgag ccgattaagc agttggataa
     5641 tagcgagaaa ggcggcaaag ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa
     5701 agtccaacgc caaccggtta ttgcgcacct gattcgcgcg acagaacgct ttaacgatcg
     5761 gctgggtaac cagtttggtg cggctatcac ctatttctca tttttatcga tgatcccgat
     5821 tttgatggtg tcgtttgccg cagggggctt tgtgctggcc tcccatccga tgttgctaca
     5881 ggatatcttc gacaaaattc tgcaaaacat cagcgatccg acgctagccg ccacgttgaa
     5941 aaacaccatc aacaccgccg ttcagcagcg tacgactgta gggcttgtcg gcctggcggt
     6001 ggcgctttat tctggcatca actggatggg taacctgcgt gaagcgattc gtgcccagtc
     6061 gcgcgatgtc tgggaacgct cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg
     6121 tgattttatt tcactgattg gtttgttaat tgcgctgatt gtgacgctgt cgatcacctc
     6181 ggttgccggt tcggcgcagc aaatgattat tagcgccctg cacctgaaca gcattgagtg
     6241 gctgaaaccg acgtggcggt tgattggcct ggcgatttcc atcttcgcca actatctgct
     6301 tttcttctgg atcttctggc ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg
     6361 cggaacattt ctggctgcta ttggttttga agtgattaaa atcgtgatga cctacacctt
     6421 gccatcgctg atgaaatccc cctctggcgc agcatttggt tccgtgctgg ggctgatggc
     6481 atttttctac ttcttcgccc gtttgacgct gttttgcgcg gcgtggattg ccaccgccga
     6541 atataaagac gacccgcgaa tgccagggaa aacgcagcct taaaataggc cggatgcggc
     6601 gtaaacgcct tatccggcat acgattttat tcggcctggc tccccgtagg ccggataaga
     6661 tgcgccagca tcgcatccgg ctataatgcg cacataacct cttgaaactc ttccccagag
     6721 cctcttcagc catctatttg ggagcaaaca atttcattcc aactcataac cccagcatat
     6781 aaatccagtt ggtaactttt atttaacctg aaaccagttt tatccactat ttataaaatt
     6841 atgtgaagca tttcatagaa gaaaaatcac tggcctaaac attatcccct ttttgcctgg
     6901 tttttgacca tttccgcgat ttgttacaca ttgaaatatc acttttgctg tgcgtaatat
     6961 ggctattcgt tagccaaaaa ataagaaaag attatgcaag caacagccac aacactcgac
     7021 cacgagcaag aatacacgcc gatcaactcg cgtaataaag tccttgtcgc ctctctcatt
     7081 ggcacggcca ttgagttctt cgacttttac atttacgcca ctgcggccgt tattgtgttt
     7141 ccgcatatct tcttcccgca gggcgatcca acggcagcaa cgctacagtc gctcgccacc
     7201 ttcgccatcg ccttcgtcgc gcgccccatt ggctctgccg tttttggtca ttttggcgat
     7261 cgcgttgggc gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg
     7321 gtgattggtc tgctgccggg ctatgccacg attggtattt tcgccccgct gctgctggcg
     7381 ctggctcgat ttggtcaggg tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg
     7441 gcgactgaaa acgccccacc gcgcaaacgt gcactgtatg gctccttccc acagctgggc
     7501 gcaccgattg gcttcttctt tgccaacggc actttcttgc tgctttcctg gctactgacc
     7561 gacgagcagt ttatgagctg gggctggcgt gtgccattta tcttctcggc ggtgctggtc
     7621 attatcggcc tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct
     7681 aaagcgaaaa aacaggtgaa gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta
     7741 accgtactgg gtacgttcat tatgctggca acctatacgc tgttttacat catgacggtc
     7801 tactctatga cctttagtac cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa
     7861 gtgttgtgga tgctgatgat ggcagttatt ggttttggcg tgatggtgcc agtcgccgga
     7921 ttactggcag atgcctttgg tcgccgtaaa agcatggtaa ttatcaccac gctgatcatc
     7981 ctgttcgcgc tgttcgcctt taacccgctg cttggttctg gcaacccgat tctggttttt
     8041 gccttcctgc tgctggggtt aagtctgatg gggctgacct tcgggccgat gggcgcgctg
     8101 ttaccagagc tgttcccaac agaagtgcgt tacaccggag catcgttctc ttacaacgta
     8161 gcgtcgattc tcggggcttc cgttgcacca tatatcgcag cctggttgca ggctaactac
     8221 gggttaggtg cggtggggtt atatctggcg gcgatggctg gcttgacgtt aatcgccctg
     8281 ctgctgaccc atgagacgcg acatcagtcg ttgtaagtat cgtgtgggca gccaccctca
     8341 ccccagccct ctccctgaaa gggagaggga gaaaagcgga gcgagatgtt atatcgcgaa
     8401 cataacagcc ctcccccaga aagtgaacgg aaaagcggta ctcgttgttt caacacgcac
     8461 aaaacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacac gctgctcccg
     8521 aactcaccag caaacacaat ccaacctcat cacttcttca tctgcgacaa tatcgtccga
     8581 cactgattag cctccccttc ggaaggtgag atcagtgcca gtaacgccgc cgccggtgtt
     8641 accagcgttg ccagtgccgc agcaacagca ccacgggcaa tcaacggccc ggctttcacc
     8701 ccagcctgcg gatttttaaa cgtcccacgc acatacagcg gcgaacgcag tgtgataatc
     8761 cgaatccctt tactctccgg atcaatagtc aaatccagct gttccgaagc aaaacttgcc
     8821 gtgccggtaa cattaatcaa cgcgttctca gtatcgaaag caaaaatctg cgggcgcgcc
     8881 acgccgttgg caatattcag attcgccgcc gcgcagttca cccgcacctc atcgtcacca
     8941 aatatcgcac cgacaatgta gttgccgaca ttcagcccaa caatctccat caggttgcgg
     9001 ctcaccagcc cgtcattcat caacagtttc aggttgccgt tactgttgcc taaaagtgct
     9061 gccaccgagt taccgctacc gcgtagttcc gcgtcaccgt tcatttcccc cagcgtcttc
     9121 tgcatcagtt ccacatcggg catcagttct ttcagtttca gtcgacgagc ctgaatatct
     9181 gcccgcccct gcatcggctt tttatcgcct tccagatgaa tattcgccgc aatgctgccg
     9241 cccgccatgc caaatttcag cggttgcagg cgcaggtcag catttttgag gatgatatga
     9301 gtagaaagat cgctaatcgg caggctactg ccatgctcaa tgcgccgccc tttgaagcga
     9361 acatcggcat ccataacgtc ccatttatcg gtttcgaagc ggtcataagg cagcaccttg
     9421 cccgcaggct gaacgctttt ttcgcccttc ttctgttcag accgtttcga cttttctgcc
     9481 cctttcccgg agtcaacgcc aattaacggt cccaggtccg ccagccgcaa ttgccgcgac
     9541 tcgacatcac cttccagttt tggtcgtggc tttccggtgg tgtagaccag agaaccgtgg
     9601 atatcgctat cgccaattcg cccattaaaa ccgcgataat caaagaccga cgatttttca
     9661 gtgtcgattt tcgctaccag ccgaccatcc gtttcaaacg gcggggtatc gggcagcaga
     9721 acgcccgtca gttcatagag atcacccagt gaatcgccag aaaatttaag ccgtaaatcg
     9781 acaccgccca tcttcattgg gtcattcacg acgccatcaa aagcaacacg ggtgttacca
     9841 gagcggaaat cagcctgtac cggaaacggc gtcccttcgc cacgcagcgc cagcataccg
     9901 cctatttttc ccgtacccgt gagcggttca ccgttataac gtccctgcgc cttcaggcca
     9961 aaaacgtaat cgcccacctt ttctttatcc gctttacctt tcgatccagt aacttcgctg
    10021 aacggcagcg gcttgcctaa cggatcaaca aaaatctcca gatccgcttt gcttactttg
    10081 tcatcaatgg cgatccgccc ttgatcgaaa agaatattat ccagccgaaa cgaccatgcc
    10141 gacggctttg cattcgcgtc tttgttatca tcgttggcga gattaaacgt ccagttattg
    10201 ttcttttcgg agaggcgaat caggcgcgcg tcgggctttt cgagcttgat ccacggcagc
    10261 cagaccgttt tggtcagcag cgccagcggg gccagcgttg cctctacgcg tggcaaatgc
    10321 accatcgtga cttcgggaat atccggtgga ttgccaagaa tgatgtcttc cgcgtgtaca
    10381 tggggccacg gcacccagct gcgccagcca gtttcttgtt tttgccgctc ccacaccacg
    10441 cccagatcgc cacggatagc gaacggacga ttcaactccg cagagacttt ctggttgatg
    10501 gtcggtttga gtcgattcca gtcaaaagtt gcaatcaaaa tgatcgccac gacaatcaac
    10561 aacaagaaag ccactgaaat cgcagcggtt attttgcctg ccttgctcat cgttcgcttc
    10621 ctcatattct tcctgtgcca gtcctaaaga tagtccagcc aggcggaaaa tgaggcagct
    10681 tatagcgcca gaaccgccgt attcagcgtt tctatcggtg ccgggcgtga aagaaaccag
    10741 ccttgtgcgg cgaatgcggg cgagttctga acatcacgcc actcttccgg cgtttctacg
    10801 ccctcgacaa tcaccccgcg acaatagcga ttcattagat gtaaaagctg agaaaagagt
    10861 gtgcgtcctt ccggcgactg acgcagcatc acaaacagtt ctcgcgcgat tttgatgtag
    10921 tcataacgca cttcacttag tgcagagaaa tttgccatcc cggtaccaaa atcatccagc
    10981 cacagcgggc caaattcaca catcgaggca aaggttgaat ctttcggcag acggatatgc
    11041 tccaccagtt cgaaacgcag ccagggaaga cgctcaatct ggcgcaggat ttttggttgc
    11101 tgacgcaggg cgatgagcgt agggccatca atattgaccg atgccagcag gccgtgctct
    11161 ataaagaagt cggctttttg cgccagcaaa tcaatctgct ctttcacaac ctccatccga
    11221 tggctgacgg tgatttcagt aaaatagcga tccggcggca ggcgttgcga agggttcaag
    11281 ggatgcgtga ccaccgttaa tagctccacg gccattaacc gtccgcatgt ttgatagatc
    11341 ggctgccagg tgtaagcacg ctcacactgc aaccaaaaac gccgttcctg caagctctcg
    11401 atgcttgctt cagggttgct tattcgctgg ataacctgcc ttatcatcaa agatgtcctg
    11461 tgtttaaaag tgatgctgcc cggactcgac aaagattatc ggcgcgcaag ggcagaactt
    11521 taccgcctgg ccggggtaaa tttcctcact ccacaacgga atctttcagg aatacgttct
    11581 ggctcaaaaa aataatggaa cactgtttta atatggttga ccagcaaacc accacagcgc
    11641 aaactaacgc taatttttta cagatcaggt tcacgactat gtccaaaaag attgccgtga
    11701 ttggcgaatg catgattgag ctttccgaga aaggcgcgga cgttaagcgc ggtttcggcg
    11761 gcgataccct gaacacttcc gtctatatcg cccgtcaggt cgatcctgcg gcattaaccg
    11821 ttcattacgt aacggcgctg ggaacggaca gttttagcca gcaaatgctg gacgcctggc
    11881 acggcgagaa cgttgatact tccctgaccc aacggatgga aaaccgtctg ccgggccttt
    11941 actacattga aaccgacagc accggcgagc gtacgttcta ctactggcgg aacgaagccg
    12001 ccgccaaatt ctggctggag agtgagcagt ctgcggcgat ttgcgaagag ctggcgaatt
    12061 tcgattatct ctacctgagc gggattagcc tggcgatctt aagcccgacc agccgcgaaa
    12121 agctgctttc cctgctgcgc gaatgccgcg ccaacggcgg aaaagtgatt ttcgacaata
    12181 actatcgtcc gcgcctgtgg gccagcaaag aagagacaca gcaggtgtac caacaaatgc
    12241 tggaatgcac ggatatcgcc ttcctgacgc tggacgatga agacgcgctg tggggtcaac
    12301 agccggtgga agacgtcatt gcgcgcaccc ataacgcggg cgtgaaagaa gtggtggtga
    12361 aacgcggggc ggattcttgc ctggtgtcca ttgctggcga agggttagtg gatgttccgg
    12421 cggtgaaact gccgaaagaa aaagtgatcg ataccaccgc agctggcgac tctttcagcg
    12481 ccggttatct ggcggtacgt ttgacgggcg gcagtgcgga aaacgcggcg aaacgcgggc
    12541 acctgaccgc aagtaccgtt attcagtatc gcggcgcgat tatcccgcgt gaggcgatgc
    12601 cagcgtaaac gcagcatatg caaaaggctg gataaagtgt tacccctcta tccagctcat
    12661 ccataaatac ctgatggttg tgctaactca tcaccatcag gcattactgt gcaggtggaa
    12721 tatccgtcac ttcaggatgt acgtcatccg tggcgacaga ggtggcggca gcagcagtcg
    12781 atggggccat gatttgatcc caggccgcct gcaacgcttt catgttaaat tccggctcgc
    12841 ctttcggctg cagcagtatt aacgccatat cattcgacaa ctgctgacgc agatcctgat
    12901 ttaacatctc caccgtcagg ctgttaagga aatcctgccg cagtttctga tactgctccg
    12961 gcgcgatatc gacgacctga ttttgcaacg aacgcatccg ctgacccatc agaatatcgg
    13021 tatcagcccg cgcataggcg gcaaacagtt tctgcagctc cagtttcttt tgcgccacta
    13081 acgcattgaa ctcttcttcc ggcagacctt tatcgcgaac cttcgccagt tcacgcgcca
    13141 ccagattaag gttgctgttc agcttgtcgt ttggtgattc gatgttgatg gcacactgcg
    13201 cacgcagata cagcacacgg cagtcaaatc caagaccgat gtctttgctg ttactggcgc
    13261 ttaacgcttg ctgaacatgc cagaacagcg cctcgcgggc caggtccgca cgccagtagc
    13321 gcagcagtgc ggctgattca cgaatcggct gccacggcgt atcccacatg atagataacc
    13381 ggtcctgacg caccgcgtca gtcataatac tcaccgcttc cgcacgcagc ggagaaagcg
    13441 tcggcaccgg agctggcgtt tcacgtttgc ctttcagttc gccaaacgtt ttgtttattt
    13501 ggtcgacaac cgagcgcgca tccacgtttc ccaccaccag tagcgtcatt gcatccgggg
    13561 tgtaccattt ctgatagaaa tctttaattt tttccgcttc aacgggttgt ttcagcggat
    13621 cggcaggatc gtgacctaac aaggttgacc ctttcagacg atagcgccac cagccctctt
    13681 tagtatcggc aggccaggtt gccaccatgt cctgactttg cagcgcgcgg ttgatggttt
    13741 ctggtgtgat ggtcaatttg ccagtggcat ttgccagata agagagcgct tctttcagca
    13801 aatcgttacg gttattgggc aaactcagat taaacagcgt ggtgtcataa gagacaatta
    13861 ccggcggcat cgggcgttta gggtcgatcc cctgctgcca caatgaacgc gcctgtgctg
    13921 cgtcaaggcc accgctttgc gttagcgcaa tacgagggat ggcgtgactg taaccgctct
    13981 gttgtgtact ttcggtgagc gaaccggtat taaccagcag gcgaatttca acacgatcgc
    14041 tgggacgctg gggggtggtc agcacttgcc actgtaaacc gttggaaagc gtcccctgtt
    14101 gccatgctgg atcaggctgg agcgcatctg cctgcacata gccagcagtg gccatcatca
    14161 gcaaaccgcc cgctaaaagt cgaatttttg tgccctgcat gtgaacccct gatcaactat
    14221 cctggtaata aaaaaactgc ccgttgtcgg tcagtctaaa tgacgttaaa aacacttcgt
    14281 gttagaccgc aagaacatga aaatgtcacg gaagaagtga aataaacctg aactcaccca
    14341 ggtggtaagt tcaggcacag gggtcaatta tgcgcaaaca cccgcactcg gggaagggag
    14401 tgcgggcata agtggtgaga ttaagaggat aattcgtgcg ttttgccatc cggcgcacga
    14461 ttattcagca catcgtccag ttttttgtgg tccagttctt tcacccactt agcaacgaca
    14521 atggtcgcta cgccgttacc gaccaggtta gtcagcgcac gagcttctga cataaagcgg
    14581 tcgataccga ggatcagcgc cagacccgct accggcaaat ggcccaccgc agagagcgtc
    14641 gccgccagca cgataaagcc actacccgtt acccctgccg cccctttaga agaaagcagc
    14701 aacacgatta acagcgtgat ttggtggacg atatccatct gactgttagt ggcctgggcg
    14761 ataaacaccg ccgccattgt caggtatatc gatgtgccat caaggttaaa cgagtagcct
    14821 gtcgggatga ccagccccac caccgattta cggcagccga gtttctccat cttgtcgagc
    14881 atacgcggca gcgccgactc ggaagatgaa gtccccagta caatcagcag ttcttcacgg
    14941 atgtagcgga taaatttgaa gatactgaaa ccagtcgctt tagcgattga acccaatacc
    15001 agcaccacaa acaggataca ggtaatgtag aaacagataa tcagctgccc cagttgcacc
    15061 agtgtgccga cgccgtattt accgatggta aacgccattg ccccgaacgc accaataggt
    15121 gccagacgca tgatcatatt gatgatgccg aagatgacct gcgagaaact ttcgatcacg
    15181 ttaaaaatca gttggccttt gctgcccaga cggtggagcg caaaaccaaa cagtacggca
    15241 aacagcagca cctgcagaat gttaccgctg gcaaatgcgc caatgacgct cgccgggatg
    15301 acatccataa tgaaggcgac aatgccctgg tctttcgcct gatcggcgta aaccgctacc
    15361 gctttcgcat caagcgttgc cggatcgacg ttcattccgg caccaggctg cacgacgtta
    15421 acgatgataa gaccaataat cagcgcgatg gtactgacaa tttcaaagta aagcagtgcg
    15481 actgcgccgg tacgaccgac cgccttcatg ctttccatgc ccgcaatgcc cgttacgacg
    15541 gtacaaaaga tgacaggagc gatgatcatc ttaatgagct taacgaaccc gtcgccaagc
    15601 ggtttcattt gctcgcctat ttcaggatag aaatggccaa ggagaatacc aatggctatc
    15661 gctgtcagga cctgaaagta aaggctttta aacagagagg ttttcatagg gtgtccttta
    15721 gtaaaaccac aggtcttgta aggttatggg gtacctgcgg ccttaaaata acacccagac
    15781 aacatcacag aaatgtacct ggatcataat tgaaacaaaa aggttaaaaa gtttgagctg
    15841 gctcgcacaa accagcactt tttaaagttt tgtaatcagt ttggggtagc tacttttctt
    15901 ccaggtaact ctcttcgaag atttcaatag ggagtgggcg agcaaaaagg aagccctggg
    15961 caataccaac gcccgctttt gccagccagt cgcgttgtgc ttcagtctcc acgccttcgg
    16021 caatcatttg taagttcagg ctctgcgcca gcatgataat tgcagcaatc atgctgctat
    16081 cttccggcaa gccttcaaca aacattttgt cgattttcag tacgtcgatt ggcaacgatt
    16141 tcatatgctg cagctgacgc agccctgcgt agcccatgcc gaaatcatcc agcgccaccc
    16201 gaacgccggc attgcgcagc ggacggagga ttgccaccgc agcatgaggg tcgtcaatac
    16261 gtcggctttc tgtcacttcc agaatcagtg ttcccggctg aatgcgatag cgggttaaca
    16321 gttccagcat atccgccacc atattcgggt gcatcagttg cagcgcagag aggtttaccg
    16381 acaagggcag cataatgccg cgctcttgcc aggctgcaag caatcgacag gactcttcca
    16441 gcacccagtg accgacggta accatcagcc cacagcactc aatgcgatcg attaagccat
    16501 ccggcaggtc ccaactgcca tccggttgct ggatacgcag taacacttcc gcactgacca
    16561 gttttccgct ggtcatctcg acctgtggct gtaaccaaat agcaaactga tgattttcca
    16621 gtgcattaag gatatcgctc tcttccgtca accgcttctg ggcggcttcc atctgctgcg
    16681 gatcaaagaa ctgaatctga ttcttgcctt tatggcgagc ggtaaatgcc gcagaaatag
    16741 cgcgactgta aagctgttcg gcggtgagat cgccgtagaa catcgccacg ccaatgctac
    16801 agtgcggacg gagttgaata cgttcaatcg gcaggcgctc gctcatgata gtgagcactt
    16861 gctgacctaa ggtgattgcg tgccacggtt cctgtacacc gttggcaatg acagcaaagt
    16921 cataaccgct aatctgcgcg aggatcatac gtggcgacag taccgatttg agtttttcca
    16981 ccagcgtcag cagcagaatt tctcgttgcg cctctttcag cacgcccgca gtatcacgca
    17041 gggtttcaca ggtgatgatc atcagcgcgg tggtttgttt acgcgcgaca acttgctcca
    17101 gcatctccat cagcaaggct ttgttcggca aatccgacac cgggaagcgc atcgcatttt
    17161 cgttctgttc ttcataatgg cgctgcagca attgctggtt gaggttgtaa ctgcgcacca
    17221 acataccgat ttcatcgtcc tgatgcagac gcggtaatgc cagttggtga ccaacaagct
    17281 cctgggctgg gatggcgtta agttcgcgag caatattgcg taacggatgc aaaatcaggc
    17341 ggttaatgca ccagctgatg gcgacggtca ggataagcga caaaagtaag taaatggtca
    17401 ctaacgttga gagggtgctc atcacgaact tatacatacg gaaggaatcc gcctgtagta
    17461 ccagataggc aattggctgc gggtttgccg gacgttcgag cgagtaaacg cccagcgaga
    17521 tttgaaccgg tagctcaaac aggcgagtaa ccattaccgg caccgggcgc tctggaataa
    17581 aacttttacg cagcgcctgg aactggttag gcagcactac atcggcacgg ctgaccacgc
    17641 ctgccggttt aatgctggca agaatagctt ccgcttcggg aatatcgcct ttcaaaatag
    17701 ctgaagataa gggttcgcgg acagagcgag caatgctttc cagttgcgta gccgtgttat
    17761 agcgattctg ctggaccaga tggaacagca aaacggtgca aaaaataaaa acgaacacca
    17821 ggacaacggc tgccaccatt gccatctgct tgattgttaa cgagcgactt acgcgcaaat
    17881 tgtctccccc cggaaatccc agattgccgc ccgagtatac ccgatcgcag cggcattaag
    17941 agaggcgcta tctgaaaact taccagtcgg cgtaaggtat cagcggctgc ggcggtaaat
    18001 ccatgtcacc ctgccatccg gcggcggaat aacgtacgta gagcaggaaa tggctgggtg
    18061 cgtaatcttt cgcctgctgg atatcaattg ccgtgccaac aaaccagttg gaagtaacac
    18121 gtcgttcaag taatgcccgc gccgtgtagc cgaagccctg actgctgccg ccatcgttgg
    18181 attgccgcgc agcttcttcc tgccagtcgg tcgggatcag attcatcagc ggataacgcg
    18241 gcatggtttt ggtgcgtgaa tgcgaccacg agccagacgc ccccagctcc cacgaccagt
    18301 tttccgtgcg ctcccgccac atcaccggta tggcaaacga caggtattcc tgcggactgt
    18361 agtaaccgcc ctgaccgagt gagtagccac tcagatcttt gtcgtaatgc cagatcatgt
    18421 tgttcaggcc gattgtgacg cggcgattgt tctggttaat gaccttatag taatagcccg
    18481 tcatccagcg cacgcgccag ttatcttcga catttttacc ggttaactgg tcgccactaa
    18541 gcgatgccca gacgccgttt gcttcacctt tatcgtagct cagacttagc cccacaccgt
    18601 cggcacgtac gccaccccat tttttcccgg tattgctcgg ggagtctttt tgcccaccaa
    18661 aggccagcaa agaactggag atgggccgac ggtgggcgtt aacggtgtaa cccagcggcc
    18721 cgatatcatc gctgtaactg atgccgccga ccacatccac cacgttgaag cccatcggcg
    18781 tggtaccgat atcccagctc cagacgtcat ttcgccagcc gaccgccacg ctggcaccgg
    18841 aatccgactg gctgcggttg ccgctacagt cctgtaatgt acaggtgccc cagttatcat
    18901 cccatttacc atcggcatta gtggagaaac tgccgacgtt catattgacg aaatcactgc
    18961 gaaagaacat ccgcccgtca gaatacggcg catccacctg caacatggta gtgtgcgctt
    19021 tcagatcgga gtaaccaccg gtgccgctcg aaccccagta atcatgctcg agggtgacgt
    19081 taagatcctg ctggcgatag aggtccgccg catcgctgcg cacgccgcgt ttcagccagt
    19141 catctttctc gtcgttacgg gtcagtcggg taaaggtgtc gttatcctgc ggacgcgtcg
    19201 tggtcacacc ggatgcgacc atggcgtctt tgtaggtttc cagcgcctgc gttggatcgc
    19261 ccgcctgcgc ttcaaacttc gcaccgtcgc gcagcaccat cgcgctttcc atcgacggtg
    19321 gctgagattt tgcctgcggg atcaacttat taaacgtctg ctgcgccgct gcggtatctc
    19381 caagctgcgc cagcgccacg cgccgctgtg tgttcagcga ggcgttatcg gtagcgggca
    19441 gtttcgccag ctggctacgt gccgccgctg tgtcaccggc agcaatatcc acttccgtca
    19501 gccccagaat ggcatcggcg ttagttggct ccagcgtcag gacattctga tatgcagcgc
    19561 gggcggcggt gtaatcacgt cgctgctgcg cccagtcagc cagcgtgagg tcaatacgcg
    19621 tggaaggtgg ttgctgacgc agcatcgctt ccgcttcagc ttctttgccg ctttctcgca
    19681 ggcgattagc ggtttccagc acctgatcgc tttgcagtcg attaaccagc tcctgaatat
    19741 tgctgttcca ctgcgcgcgc ggcaggctgt tgatatgcgc cagcgccgct ctgtcctggt
    19801 catgaccaga gaggtacagc ccgtaagcgt aaacctgctc cgggtcgttc ggcttctgct
    19861 gcgccagatt gcgcattaac gtatcggcct ggctgcgttg tccggcctgc cagagatcct
    19921 gcgaaagtcg gtaagtaatc catacgctgc cggggtccag cgccagtcgt tgccgctgaa
    19981 gtgctgccgc ctgcgcccat ttgccctggt tttccagtgc ctctgcctgc tgtgccagac
    20041 ggtcgttttg caggctgcgt tcgatatcat caatgctacg ccgctgactg gcagagagcg
    20101 aggcgataaa cgcttcagct ttttctggcg attgctggcg gtaaatattt gccagcccgc
    20161 gcacggcgtt agtgttgccg ctgtccatac gcaaggtctg ctgataataa cgttcggcgg
    20221 cgggataatc ttttcgcgcc atcgccacat cgcccagccc cagcactgca taactgtcgg
    20281 tgttatcgac attacgcgcc tgctggaaca ggcgttctgc ccggtcagga ttattggctt
    20341 tcagcgcagc atcgccctgc tggatcgcca gccagtagcg gtttactttc agcagactgt
    20401 tccatttgtc gttgttgctg ctgtgcgggt ccagtgcgag ggctttttcc agattcgcca
    20461 ctgcattggc gcgatcgcct ttctgagaat acgcctggcc cagcgccccc agagcttcac
    20521 tgtctttcgg gttcgcccgc accgcctgtt gtagttcggg aatggcttta cccgccatac
    20581 cagagtccac cgccgctaaa ccttgcgcac gagcgcggaa agcaggatcg gccagctgtt
    20641 tttgctgttc tgccagttgc gattgcgcag ccgccacgct atcgccatca ctaaagatcg
    20701 agagatattt tttcagcgcc gacacactgg catcactgac gggcatgtct ttaatctgcc
    20761 cgtaccagat tttagaggcc ccttcgcgcc cggcgttcga tttcgccatc tgttccagga
    20821 cggcaaaacc ttcgtcacgg cgatcgctac taaacagcaa tagcgccaga ttgttttgca
    20881 ggcccgtatt gcccggtgca tccgcattga tgcgttttaa ctgattaatc gcttcgccac
    20941 ggcgagccgg aattttcgcc accgtactcc agtactcgac agcaatgtca ccttccggcg
    21001 gcgcaccgtt gaacagtttg ttgtaactcg ccacagcttc ttccgcatga ccggtagtcg
    21061 cctgcaatct tgcctgttgc agtgcctgac gaccatccgg cgtggaaagt agcatcgtag
    21121 tccgcgacga tttatacgca tttgaactcg gcgctaactg cgacagccga tcgagctgtt
    21181 tttgcgcgcc atcaatatcg ccctgacgta acaaagaacg gaaacgggcg gcaacgacgt
    21241 ccgggttatt cggatcaata agttccagcc gatataacga ctgttgcacc agatcttcac
    21301 gatgggtcgc ttcgcctaac cgaacttgct ccagcaactg ttgctgagcg gttggtgctg
    21361 cctcgaccat cggcatgacg gccagaccga gggaaagcgt gaatatgttt agtgtgaatt
    21421 tgcgcattcc tggccccagt caggtaataa ctcacctttt gtcgagaagc ggaaacggtg
    21481 ttgatcccag ccttgtccaa acagggtcag cacatagttg taataggcat cgctgccggg
    21541 aaagttatcg gccacgcgct ggcgctgaac ggcctgcgca tcgcggtttt gtaaaaaagg
    21601 cagcatggcg gcagaaaaac cgaccggtcc tttaccctgc gctttccccg tagccacatc
    21661 cactttttcc ggcggataac cgtttttctc ggtgaatgtc gccatcggtt taaaccggtt
    21721 gagcatccgc gctttttgcg gatcgctgtc aggcatcatg cctacccaca tgtaaacgcg
    21781 gatagcgtcg tagctgctga tcaatgtttt ttcggctttt agctgccagc ctttgtcttt
    21841 ctcatagcgc acccagtctg gcgaaaagcc tttcggggcg gtttccagca ataaacgttg
    21901 attggtttcg cgcagcgtgg tccacggcgc gccaaagcgg gtgaaatact gcgccagcgt
    21961 cggcggcagg taactggggt taaaacgcca gctgttatcc tcagcaaatc ccactttgcc
    22021 cggcaacaac atggaaccca gcccaggcac cgtcaccact tcctcccgcg caatacgttt
    22081 taacaacgcg ctgccgatgt cggtataacg ctgctctttc cacaaacgcc ccgcctccag
    22141 caacgaccag gccatccaga catcaccatc ggaggccgaa ttgctgtcca gcacttccca
    22201 cttactgttc tctttcttgc cccacagcca ggcgggcaaa cgttctttta aagaaccctg
    22261 agcgagattg ttctgcgtcc agtcgagaat attatcgaaa gctgcacggt cgttagccgc
    22321 cagggcaaag aacatgccgt aactttgccc ttcggaggtg gtgattttgc gcgcgtcgct
    22381 ggggtcgatg acgcgccctt cctgactgat gtaatccttt ttaaactgct cccaggcagg
    22441 ccaggtacag gctgcctgaa cactaaaggc agccagcagc agcatcgtca cgattccact
    22501 acgcaacaca ttcatcttca attactcgtt atccgggtta agacgacgac gactaataat
    22561 tcgcagcaga cgccacagta cccatgccag caatatcaca ctgatagccg ccagcaccgc
    22621 cagcagaatc ggatggtttg ccagcgcata ccacacgcgc tcgaaccacg gcagatgacc
    22681 tacgtaataa acgtcgccaa cacgtaggct gttgataccg gactcgcgga tcaccgcgac
    22741 cgaaccgaac atggtggcgc gtttgccgct atcgttcacc gcatcgttaa gcatttcata
    22801 accgcgtggg ctatctgcca acagcgcaat cacgctgcgc tggtcgttat acggcgactg
    22861 gaagccaatc accgccgcca tcgcaccgga agaggtcagc gttgaccggg tttctgccgc
    22921 gcgatcgctc tcgtccggca caatgccggg gaacggggtc tggcgcatcg gtgttttcac
    22981 ccagctttcg gtcgcctgca ccaataggtc gatctgctta tcgtctttca gtttgtccgg
    23041 gataccaccg atgatcatga tgtcggcatc tttgccctga atggtgctgc catcatcggt
    23101 caccgtcaga ttaatcgccg ggaagcccgt ctgtgcgccg ataaaaccaa cagtattcag
    23161 caacgtttcc atctgtgctt cgttaggcgc tttcggcatc acggtgatgg tttgcgacag
    23221 atccgccatc cggctgaatg ggaagcccgc gttagcaaag gcgcgtagat ccggcatcgg
    23281 gatgaagtgg taatacttcg agaagtcgat ggtggagtcg tcaccaatca ccacatgatt
    23341 ctgcaccggc tggaaggtaa tacagttatc caccgaaccg cccggcatcg ggttcatata
    23401 ctcaaagtcg aagcgcagct ggttggtcgc gcccagtttc agcgccggaa tagagacatc
    23461 tgttttgcca tccagcaaac cttgtaatac cggaatccgc agcagcaggc ggttcgcctc
    23521 ctgtttgctg ctcaggttga aggattgcag gaactggtta ttcaggctga tatccatccg
    23581 cgaactgtct ttcaccggcg gcatggtgta gcggtaatta atatccatat caatgccggt
    23641 actgcgcatc aggtagagat ccggcggcag gtttagcgaa acgttaatcg ctgctggctc
    23701 aagaccgctg gattgtaact gttcttcata ggttttcagt tcgccaaatg tgaccggacg
    23761 atcggtacgt acccagttcg gcgcatcgta cggcttacgc ggtagcagcg gtttcacttc
    23821 attcactacc acgctttcac cacggaacag aatgttaccc tgagcgatac ctttcgctgc
    23881 ctgcaacagg tctttgtcgt cacgaccaaa caccaccagc agtttgacgt aaggattctg
    23941 cggatggtta atcatctcaa tcacaggggc ttttaccgcc ggatgatcgc gcaggaagtc
    24001 cggccgtttg tcgttggttg caaagacaat ggcattacga tccggcagtt ggttatagag
    24061 caccgggaag ttctgcccac gccagccaga acgcgaacca aaccacgagg cgacaatggc
    24121 agaggcttgt tgcagcccaa catccggcgc acccgcaaag accatcggca aggtgttggt
    24181 gcggttatca cgcggatcaa agaatggcac cgggaagtgt gacaggtcat tcttcacatt
    24241 cagggtctga taggtcagat ccagtccact gctccgccca acatccagcc aaagcgtggt
    24301 gctggccggg ttttcgcaca cgtcctgata atggccgaca aactccagcc gcacacggtt
    24361 gaagtcggta ataaacagtg ggttaatggg catttgcgcc agcgtttttt tacccaactg
    24421 ttctttggtc actggcagca cgcccatcag ctcatcattg agataaacct ttaactgcga
    24481 ctggacaggc agtaacgatg gcgatggcgt gtattcgagg ttgagcatcg ccttcgtcac
    24541 cacttcatcg ctgcgcatac caaactcaat gctgccgttc gggttaatgc cacgtagcac
    24601 catgctgccc ggcggcggcg caatttgcgc aaaggtcagc ttcacatcac gcgaaggacc
    24661 gttctgcgcc acgactggcg catcagcgcc ctgcacgccc ggcatcactt gccccacctg
    24721 cggattttgt tccgtctggg cggctacagc tggctcagca ttgatcagtg gttgcgttgc
    24781 tggcgtcgcc tgcgtcatga aagaggggaa cgcactcatc cccatagcca ctgcacaaat
    24841 ccagaatagt tttcttttca tcgcgttatc atcattgttg agccaaagcc tgatccgatg
    24901 gttgtgccgt ttcgctccgc tccgggcggc gcggaataaa cgatacaacc caggaaacca
    24961 gagaagtcag cacacggaat atgcccttca ccgaagaagg cgcaaactcc gccagatggc
    25021 ggtagccacg gaagccgagc ttcagaatat ccagcagact ttccagcggc ttatcttccg
    25081 ggtagctgtc ctgccagagc gcccatgtat ccgcacgggc aaacgtacac tgcacaaaat
    25141 cgatatgttg ctgggtggtg agcggcatta atttcagccc aacttcatta cccatcacgc
    25201 gcgccacctg ggtcgggaag acgtattcct gctgaccgcg tttaagcaac agattcactt
    25261 tctgcccttc cagaatctgc gcctgaccgt tgatcttgat ccccaaacca ccgtcggaga
    25321 aatcctgaac ggtacacgag aagaggtgac catcttcgcg ggcaattgcc gcgggcatcg
    25381 tcatctccac gcggtgcgat cggcgtacct gtttgctttc taccgatacc gcaactgcgc
    25441 cgccaagaac aatcaggttg tagaacaccc acaccatact gacgaccacg gtgagcatct
    25501 cggttggcgg gccatagaag tagcgccaga tgcctaccgc aacgcccacc aggttgagta
    25561 gaacaaggaa gatgtagggc cgcgagatca cccagtcgac gtactcttct tccaccagtc
    25621 cacctttggc ggtgacgtta aatttgcctt tgtgcgggtt aatcagcgcc accagcgtcg
    25681 gtggtgcgat ataccacgcc agcaccgttt cgtagatttc actccagaaa gagtggcgat
    25741 atttgccctg gatcttggag ttggtcaggc tggcatggat catatgcggc agcacgaata
    25801 gggcgatcat caacgctggc gcatagatga tgtaggcatg aagcagcagg aacgccagcg
    25861 gcgcagtcag gaagatcagt cgtggaatgc ccgacaagaa gtggaacatg gcgttgacgt
    25921 agcacagccg ctgggcgaat ttcagccctt taccggtaag cgggttatcg agacggaaga
    25981 tttgcaccat cccgcgtgcc cagcgaatac gctgaccgat atgcgccgac agactttcgg
    26041 tcgccagccc cgccgcctgc ggaatacgca tatacgcgga ggtatagcca cgacggtgca
    26101 aacgcagaga agtatgcgca tcttcagtca cggtttcgac agcaatgccg ccaatttcat
    26161 ccagcggctt acgacgaatc accgcacagg aaccgcagaa gaaagtggcg tcccacatat
    26221 cgttgccatc ctgcaccaga ccatagaaca gcgtgccttc gttcggtgtt ttacgaaaac
    26281 gccccaggtt gcgttcaaac gggtccggcg agaagaagtg gtgtggagtc tgcatcatcg
    26341 ccagctgttt ctctttcagg aaccagccca tggtcatttg caggaacgat cgcgttggta
    26401 cgtggtcgca gtcgaaaatc gacacgaact cgcctttggc atatttcagc gcattgttga
    26461 tgttgcccgc tttcgcatgt tcatgagtgg tacgggcgat atatttcacc cccacgtttt
    26521 gcgcaaactg gcgaaactct tccctaccgc cgtcatcgag gatccagatg ttcagcttgt
    26581 ctttcggcca gtcgataccc agcgaggcgt aaatagtatt tttcaccacg ttgagatctt
    26641 cgttgtaagt cgggacaaag atatccaccg acggccacag cgacatatct ttcggcaatg
    26701 gcaccggctg acgattcagc ggccatacta cctggaagta gccgagcacc agcacaatcc
    26761 acgcgtacgt ttcagcgaag agcagaataa gcccgcacac caggctgacc ggatcgtccc
    26821 agttcagcgt agaggtgtaa cgccaccaga tataacggca agaaacggtc agcgacagca
    26881 caatcaacat tagcgccgag aagcgccccg gcatccgccg tacgatcagc gctacacccc
    26941 acagcagcat caggaagata aactgcgcca gcgggttaaa cggttgagta acgcagatta
    27001 acgccagaat cagcgagaag gtgacgatga taccgaggat caaccgccgc gccccggcac
    27061 tcaaatgacc gagctctttt ttctcatcaa gatgctgtgt tttatggcta acgcgctcag
    27121 gcagctcgtt catccattga tggtaacgtc cacgaatatt ctgcagacct gaaaatgccc
    27181 gcctgcgcgg tttcggcgtt tctttgcgcg atgtaccgat cagtaaccag catgtttgaa
    27241 tgagataacg gaccgggtcc agcggacgcg gacgcgaggc gttgatatgc ggatacaggt
    27301 ttttatgttc tgcgcgaata cgctgccagc gcgggtgctc cagcggaata aaaatccagg
    27361 ccaggatcat ccagaaacag ccgagcgtcg cgctgaaagc cgacgcaccg tgacgacgat
    27421 aatcgcgata acgcccgata agccgcgcgt tgaccggcgg gataagcaac caccgggtca
    27481 ggatactcat gatgcactcc cgactggcgt tttcagcccg gagtagttca acaggcacca
    27541 gttcgccagc gtcagtatct cttcagccgc cagcgcatca ctgcgatatt cacctactgg
    27601 ttgcttagcc gccaggcatt cagccatcgc ttcatcacga tgaatgagca tcggcagtaa
    27661 tcggcgctgg ctttgcaacc aaagctggta aatatcgtcc tgaacctgac tgccaatacg
    27721 gaagtcatta atcaaaatat gtgcgccatc cggcagcgct tgctgatgca gtcggatatg
    27781 gcagttggca tccacattga cgattgccag cgagtgatcg cacaaactca gcagctggtg
    27841 ggttatctgc gaggcatcac gcggtaagtc gattaaaatc cactggtaac gcccgctggc
    27901 ttttagttgc tgtaagccgg agcaaatatc gctcagccgg gtttgccagt gctgtggatt
    27961 ttcttgttct tcaatggata actgaccaaa aggcagcaaa tcgagctgcg aggtgtagcg
    28021 caacccagcg tcacgccagt cctggccatc cagcatcgct ctggcccagc cctgacggtg
    28081 ggtaaaatca acattaaatg acaggcgcaa caagttgtcc gggcaggcat cgaccaccag
    28141 gacattttct cccaacattt gtaatgacca ggctaatgcg gcggtgatgg ttgttgtccc
    28201 cacgcctccc cgcaccccct gcaatcccag tacggccatt caccaatccc ctacttttgt
    28261 tgcgcaaact ctgccagcaa cggccagcgt tttaatgccg cggccaactg ttcgcgttgg
    28321 gaaatatcgg cataatcaat atcaggcagt gaaaatgcct gctttaacgc cacaatatca
    28381 ttctggaaga tatagcctat cgcgggatca ggcagagtat ctggttcatt gttattcatt
    28441 ttgcttgatc cctatgaaat aaccgtaccg ttcatgagac aattctaacc gtgagcattt
    28501 cgccggttta ttgtttacgg cgagacggga atttgctcac tttttgactt tccgttacgc
    28561 aacagaaaaa gttaaaaacg aaaagactaa aatcacacgt cttgtgacct tctaaattga
    28621 ttcacatgct aaatctgata agttttaatt tcaatggtag gtttatttct tagctttcgc
    28681 tagtaaactg ataatcagac aaaatggtga catgagggac attgtggacc ctgtattctc
    28741 tatcggtatc tcatcattat gggatgagct gcgacatatg ccagcaggcg gcgtctggtg
    28801 gtttaacgtc gatcgccatg aagatgctat cagtctggcg aatcaaacaa ttgcatccca
    28861 ggctgcaacc gcacacgtcg cggtcattag catggacagc gatccggcga aaatctttca
    28921 attagatgat tctcaagggc cggaaaaaat aaaattattt tcaatgctaa atcatgaaaa
    28981 aggtctacac tatttgaccc gtgatttgca gtgttctatt gatccccata attacctttt
    29041 tattcttgtt tgcgcaaata acgcatggca aaacattcct gccgagcggc ttcgctcatg
    29101 gttggataaa atgaataaat ggagcaggtt aaaccattgt tcgcttttgg taattaatcc
    29161 cggaaataat aacgataaac aattttcatt gttgcttgag gaataccgtt cactttttgg
    29221 tcttgccagt ttgcgttttc agggtgacca acatttgctg gatattgcct tctggtgcaa
    29281 cgaaaaaggg gtcagcgccc gtcagcagct tagcgttcag caacaaaatg gtatctggac
    29341 attagttcaa agcgaagagg cggagatcca accacgcagc gacgaaaaac gcattctgag
    29401 taatgttgct gtactggaag gtgcgccgcc gctatcggaa cactggcaac tgttcaacaa
    29461 taacgaagtc ctgttcaatg aagcccgtac cgctcaggcg gcgacggtgg tcttttcttt
    29521 acagcaaaat gcgcaaatcg agccactggc ccgcagcatt cataccctgc gtcgccagcg
    29581 cggtagtgcg atgaaaatcc tcgtgcggga aaataccgct agcctgcgcg ccaccgatga
    29641 acgtttgtta ttggcctgcg gtgcaaatat ggttattccg tggaatgcgc cactctcccg
    29701 ttgtctgacg atgatcgaaa gcgtgcaagg gcagaagttt agtcgctatg tgccggaaga
    29761 tatcactacc ttgctgtcaa tgacccagcc gctcaaactg cgtggtttcc agaagtggga
    29821 tgtgttctgt aatgccgtca acaacatgat gaataaccct ctattacctg cccacggtaa
    29881 aggcgttctg gttgccctac gtccggtacc gggtatccgc gttgaacaag ccctgacgct
    29941 gtgtcgccct aaccgtaccg gcgatatcat gaccattggc ggtaatcggc tggtgctgtt
    30001 tctctcattc tgtcggatta acgatctgga taccgcgttg aatcatattt tcccattgcc
    30061 tactggcgac attttctcaa accgtatggt ctggtttgaa gatgatcaaa tcagtgccga
    30121 gctggtgcag atgcgcttgc ttgccccaga acaatggggc atgccgctgc ctttaacgca
    30181 aagttctaaa ccggtcatca atgccgagca cgatggtcgc cactggcgac gaataccaga
    30241 acccatgcga ctgttagatg atgctgtgga gcgctcatca tgatgaccat cagcgatatc
    30301 attgaaatta ttgtcgtttg cgcactgata tttttcccgc tgggctatct ggcgcggcac
    30361 tctttgcgac gcattcgcga caccttacgt ttgttctttg ctaaacctcg ttatgttaaa
    30421 ccggccggga cgttacgccg cacggaaaaa gccagggcaa ccaaaaaatg actcaattta
    30481 cgcaaaatac cgccatgcct tcttccctct ggcaatactg gcgcggcctt tccggctgga
    30541 acttctattt tctggttaag ttcggcctgt tgtgggcggg atatcttaac ttccatccgc
    30601 tcctcaattt agtgtttgcc gcgtttctgc tgatgcccat tccgcgctac agcctgcatc
    30661 gcttgcgcca ctggattgcc ctgccgatcg gctttgcttt gttctggcat gacacctggt
    30721 tgcctggccc ggaaagcata atgagccagg gttcgcaggt ggcggggttc agtaccgatt
    30781 atttaatcga ccttgtcaca cgctttatta actggcagat gattggggcc atttttgttt
    30841 tattagtggc ctggttattc ctgtcacaat ggattcgcat taccgttttt gtggttgcca
    30901 tactgctatg gctgaacgta cttaccctgg cgggaccaag tttctccttg tggccagccg
    30961 gacaaccgac gaccactgta acaacgacgg gtggtaacgc agcggcaacc gttgcggcga
    31021 cgggtggcgc accggtagtg ggtgatatgc ccgcacaaac tgcaccgcca acaacggcga
    31081 accttaacgc ctggctgaat aatttctata acgcggaggc aaaacgtaaa tcgaccttcc
    31141 cgtcttcgct gcccgctgat gctcagccat ttgaactact ggtgattaac atctgttcgc
    31201 tttcctggtc ggatatagaa gccgccgggt tgatgtcgca tccactgtgg tcgcatttcg
    31261 atattgagtt caagaacttt aactccgcca cctcctacag tggcccggcg gcgatccgtt
    31321 tactgcgcgc cagctgcggg cagacttcgc acactaatct gtatcaaccg gcaaataacg
    31381 actgctatct gtttgataac ctttcgaaac tgggctttac ccagcacctg atgatggggc
    31441 ataacggcca gttcggcggt tttttgaaag aagttcgcga aaatggcggc atgcagactg
    31501 aattgatgga tcaaacaaat ctgccggtta ttttgctggg ctttgatggt tcgccggttt
    31561 atgacgatac cgccgtgctt aaccgctggc tggacgttac cgaaaaagat aaaaatagcc
    31621 gtagtgccac gttctacaac acgcttccac tgcatgacgg caaccattat ccgggggtca
    31681 gcaaaacagc ggattacaaa gcgcgggcgc agaaattctt tgatgaactg gacgccttct
    31741 ttactgaact ggagaaatcg ggtcgtaaag tgatggtggt cgtggtgccg gaacacggcg
    31801 gcgcgctgaa gggcgacaga atgcaggtat ctggcctacg tgatatccct agcccgtcga
    31861 tcacagacgt ccccgttggg gtgaaattct tcggcatgaa ggcaccacat cagggggcac
    31921 cgattgtcat tgaccaaccg agcagcttcc tggctatctc cgatctggtg gttcgcgttc
    31981 ttgatggcaa gattttcacc gaagacaatg ttgactggaa aaaactcacc agtgggttgc
    32041 cacaaacagc accggtctcc gagaactcaa atgcagtagt tattcaatac caggataaac
    32101 cgtacgttcg cctgaacggc ggcgactggg tgccttaccc gcagtaagca cgaaaaaggc
    32161 cgcagagttt aacctctgcg gcctgatccg ggcgcaaatt gccattacgg cagcctgacg
    32221 cccgcatgac acgttacttc cgcttgttca gccagttcac gatcatactg gcaagaatac
    32281 cagcaatgac cggagccgct aaatcatgcc agaaggccat gcccagttct gcgagcgtca
    32341 tatagccgcc tgtattgtaa tgacaacgtt tcgcggctat tcttgtattg tttggatgca
    32401 agaatggccc ccgtggtgtt gtcaggtgca tacctgcaac gtgcgggggt tttctctctc
    32461 cagcaaccaa tgccaccagg gattaagccc ccgcaacatt gcgcctcacc ggaatccttc
    32521 cggcttgacg ctgattctac gactgttttt ctgttccgga atccgtctca tcttcactct
    32581 gtgctgcgca ttccgcagaa tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa
    32641 ggccgcagag tttcactcct gcggcctggt ccgggcgcaa attgccatta cggcagcctg
    32701 acgcccacat gacacgctac ttccgcttgt tcagccagtt cacgatcata ctggcaagaa
    32761 tgccagcaat gaccggagcc gctaaatcat gccagaaggc catgcccagc tctgcgagcg
    32821 tcatatagcc gcctgtgttg taatgacaac gtttcgcggc tattcttgag tggtctaggt
    32881 tcaagattag cccccgttat gttgtcaggt atatacctgc aacgtgcggg ggttttctct
    32941 ctccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggaaccc
    33001 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
    33061 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
    33121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
    33181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
    33241 gcaagagaaa ctgggtttat tcatttttgc gaggccgact tctttctgga caggacttta
    33301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
    33361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
    33421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
    33481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
    33541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
    33601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
    33661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
    33721 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
    33781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
    33841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
    33901 gcgatgtcct gcttaccatt ccattttgct tcttttctgc ggtatttatt caggtactta
    33961 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
    34021 cgctgcgtac ccatcgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
    34081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
    34141 cagcactggc gctggccgcg caggtgatcc ctgggcatat cattcatatc acctctacgg
    34201 tgcttaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
    34261 ccattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
    34321 cacgcgtgct gactctggcg atctgcgctt ttatcgtcat tacgttgacg atttgggttt
    34381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
    34441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
    34501 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
    34561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
    34621 cctgatcaac agcaaaacaa gctaccagtt gaccgccgta gtcttttagc tgcggctgca
    34681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
    34741 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
    34801 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
    34861 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
    34921 caatgtgctc cacaaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
    34981 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
    35041 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
    35101 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
    35161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
    35221 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
    35281 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
    35341 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
    35401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
    35461 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
    35521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
    35581 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
    35641 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
    35701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
    35761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
    35821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
    35881 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
    35941 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
    36001 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
    36061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
    36121 ggaaagctga tgcgggtaaa catccagacg cgatgccgga tcgggaatac cgacctgatt
    36181 cagcaggtca atcgctcgct gacggcgggt acttttgttg ccgccctgat gcaccttaat
    36241 cgcttccata atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
    36301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg agatacgctg
    36361 caaatcctgg ccgttaaact ccagtttttc tgccattacg cggcccggat aatcaatcag
    36421 ccccataatc gccagtgaac tgaccgactt accggagccg gactcaccca caatcccgac
    36481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gcgcgctttc
    36541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
    36601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
    36661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
    36721 gcaacacgtc ggagagcatg gtgccccact caggcgttgg cggctgtgcc cccatgccga
    36781 ggaaaccaag agcagccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
    36841 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
    36901 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
    36961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
    37021 acgggccgaa aattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
    37081 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
    37141 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
    37201 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
    37261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
    37321 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
    37381 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
    37441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
    37501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
    37561 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
    37621 agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag accgggccac gagaagatgg
    37681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacca
    37741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
    37801 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
    37861 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
    37921 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
    37981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
    38041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
    38101 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
    38161 ccgcagccag cacaccaacc ggaataccga ctgccgtagc aaaaatcatc gcgcagacgc
    38221 caagttccag cgtggcctga aagcgcggca cgaactcttc ccatactgga attcggcttt
    38281 tcattgaaat gcctagatcg ccgtgcatta cgccccaaat gtaatggaga tactgctgcc
    38341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctca ggggagatcc
    38401 cacgttcgcc tgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
    38461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
    38521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
    38581 cacaaatcaa ttcccgccct tatcggacag ggaatgaagt attgctgccg gatggcgctg
    38641 cacgcagagg cacagcgcca ccgggtatac agattgttga caaagtgcgc tttgtttatg
    38701 ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg tgcaaattca atacattgca
    38761 gaattttcgt aggcctgata agcggagcac atcaggcagt tttgcctttg ccatcagtct
    38821 tgtatggctt ttaattattc gatagagaca ttttcgaagt gatgtttgcc taatggatca
    38881 accacatagc ctttgacttc tttgcgtacc ggttcaaaca cggtggagtg agcgatgatc
    38941 agtgccggag cctgatcgtg catcaccacc tgcgcttgct tgtacagttc aacgcgtttg
    39001 ttatggtcgt cggtagcacg cgccggttga atcagatctt caaacggttt gtagcaccat
    39061 tttgagtagt tagaaccttg ttcagaggcg gcgcagctga acagggtggc gaagaagtta
    39121 tccgggtccc cgttatcgcc agtccagccc atcattaccg tctgatgttc gccatctttc
    39181 gcacgcttga ggtactcacc ccattcgtag gtgacgattt tggcctgcac gcccactttc
    39241 gcccagtctg cctgaatcat ctccgccatg cggcgagcgt tcgggttata cggacgttgt
    39301 accggcatcg cccacaggtc gatggagaaa cctttttcca gacccgcttc tttcagcaag
    39361 gctttcgctt tttcaggatc gtaggtgtag tcctgaacgt cgtcgttata gccccacatg
    39421 gttggcggga tcaggttttt cgctgataca cccgcgccct gataaaccgc tttgatgatc
    39481 gcgtctttgt tcaccgcgta ggtcagagcc tggcgaactt ttacgtcatc gagtggtttt
    39541 ttctgcacgt tatatgaaag atagccgacg ttcagccccg gcatttccat caggttgatg
    39601 gatttatcct gcttcatgcg agcgatatct gccgggttcg ggtacggcat cacctggcat
    39661 tcattcttct gcaatttcgc gtaacgcacg gaagcatcag gggtaataga gaagaccagc
    39721 gtatcgatct gcggtttggt gccccagtag ccatcaaacg ctttgtagcg gatacgggaa
    39781 tctttttggt actgctgtaa ctggaacgga ccggttccga ttgggttgag gtccagtttt
    39841 tccggtgtac cggctttcat catcgcatca gcatattctt ttgacagaat agaggcgaag
    39901 tccattgcca gatcagcgag gaacggcgct tccgggcgag tcagtacgaa ctgaactgtg
    39961 ttgtcgtcca cctttttcac ttcactgatc agctccggca agcccatgcc ttcgaagtat
    40021 tcgtagctgc cgccagagac tttatggtac gggttttgcg cgtttttctg acggtcgaac
    40081 gagaacacca cgtcatcggc gttcagttca cgcgtcggtt tgaattcttt attatcgtgc
    40141 cacttcacac ctttacgcag atggaaggta taggttttac cgtcttcgct gacttcccac
    40201 ttttcagcga ggcccgggat cacttcagtg gtgccgattt taaattcaac caggcggtta
    40261 taaagcggta cggaagaggc gtcataggtg gtgccggagg taaacagctg cgggttaaac
    40321 ccttccggag atccttctga gcaataaacc agagttttag cctgaacact tgctgcgacg
    40381 gtcatagcca ccaggctgag accaagcttc agcatccctg actttttcaa ggaaatacgc
    40441 attattctgc tccaattgtg atgtttgttg ttttaaccct ttgcagtggg ttgtcgctgc
    40501 ctgacctttt ttgttttttg cccggtcggg tcaacgttat gaggtgggga tgccgtacta
    40561 attaacatca gtgtaacagt gcggatcctc cataaaatgc ccctcgtgac ctacaatctg
    40621 tcaacagaat gtgaaaacgt caatacagcc aaccgggatt tacaccaacg gtgagaatcc
    40681 acaaacaaag attaaaaaaa cttcaaacag ctatttgcag cagagaaatt tgtgctactc
    40741 caacatgacc agaacaatca gcttaatatt tagcaacatt tggtgattaa gttttatgca
    40801 tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc gattaatacc atgaacgcta
    40861 aaacgcacag cggaaaatgc cagggtcagc cataagtaac gcaaaaaaag atttaagcaa
    40921 atataaaaaa agacaatggt ttctgtcaca aatccgttaa tggatagtga gatatggggc
    40981 gcaaatttgg cagggaaatg tgtggtgtgt aggtcgaata aggcggtcgc gccgcatccg
    41041 acaataagtg cggtgccgtt gcctgatgcg acgctggcgc gtcttatcag tatctacgtc
    41101 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
    41161 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
    41221 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
    41281 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
    41341 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatgcgt aacaaaaagc
    41401 cctacatttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggattgccc
    41461 atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac
    41521 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
    41581 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
    41641 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
    41701 tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa acggtagttt
    41761 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
    41821 tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta
    41881 gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc aattagttga
    41941 attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac cttcattgcc
    42001 cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga catgatcgat
    42061 tccgcggccg tgactgtcac cgggtatttc tgctcagtta ctgttacgag ccccgtaaac
    42121 cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt cgcgatactg
    42181 cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac tctcgccacg
    42241 aagaataacg tcatccccgg tcgctcatgc tgatgtggca ctcatctgca aacttagtca
    42301 gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg tgtacaacac
    42361 gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa cctgtcgtga
    42421 attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga atggggtagt
    42481 tacgctagag agttaccgaa ggagaagcat ctgactggta agatttttac tcagcacatt
    42541 gagcgtaaca atctgaccct gagaacccga atcaagcgtc tggcccgcag aaccatctgt
    42601 ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt
    42661 tctactaatt ggagtcatca cccttctgat aagctttcct tgga
//
LOCUS       DAZFAH010000018        42192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFAH010000018 DAZFAH010000000
VERSION     DAZFAH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.018"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1816
                     /gene="adeD"
                     /locus_tag="ACHJWB_000920"
     CDS             50..1816
                     /gene="adeD"
                     /locus_tag="ACHJWB_000920"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4312599.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1861..3252)
                     /gene="uhpT"
                     /locus_tag="ACHJWB_000921"
     CDS             complement(1861..3252)
                     /gene="uhpT"
                     /locus_tag="ACHJWB_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4312600.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIRGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3390..4709)
                     /gene="uhpC"
                     /locus_tag="ACHJWB_000922"
     CDS             complement(3390..4709)
                     /gene="uhpC"
                     /locus_tag="ACHJWB_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4312601.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4719..6221)
                     /gene="uhpB"
                     /locus_tag="ACHJWB_000923"
     CDS             complement(4719..6221)
                     /gene="uhpB"
                     /locus_tag="ACHJWB_000923"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4312602.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6221..6811)
                     /gene="uhpA"
                     /locus_tag="ACHJWB_000924"
     CDS             complement(6221..6811)
                     /gene="uhpA"
                     /locus_tag="ACHJWB_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4312603.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6886..7176)
                     /gene="ilvN"
                     /locus_tag="ACHJWB_000925"
     CDS             complement(6886..7176)
                     /gene="ilvN"
                     /locus_tag="ACHJWB_000925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4312604.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7180..8868)
                     /gene="ilvB"
                     /locus_tag="ACHJWB_000926"
     CDS             complement(7180..8868)
                     /gene="ilvB"
                     /locus_tag="ACHJWB_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4312605.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8974..9072)
                     /gene="ivbL"
                     /locus_tag="ACHJWB_000927"
     CDS             complement(8974..9072)
                     /gene="ivbL"
                     /locus_tag="ACHJWB_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4312606.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9637..9726
                     /gene="tisB"
                     /locus_tag="ACHJWB_000928"
     CDS             9637..9726
                     /gene="tisB"
                     /locus_tag="ACHJWB_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4312607.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9989..10063)
                     /gene="ysdE"
                     /locus_tag="ACHJWB_000929"
     CDS             complement(9989..10063)
                     /gene="ysdE"
                     /locus_tag="ACHJWB_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4312608.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10145..11329
                     /gene="emrD"
                     /locus_tag="ACHJWB_000930"
     CDS             10145..11329
                     /gene="emrD"
                     /locus_tag="ACHJWB_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4312609.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLCHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11337..11834)
                     /gene="yidF"
                     /locus_tag="ACHJWB_000931"
     CDS             complement(11337..11834)
                     /gene="yidF"
                     /locus_tag="ACHJWB_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4312610.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDSTLQKWCGSENP
                     QSRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11831..12193)
                     /gene="yidG"
                     /locus_tag="ACHJWB_000932"
     CDS             complement(11831..12193)
                     /gene="yidG"
                     /locus_tag="ACHJWB_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4312611.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12183..12530)
                     /gene="yidH"
                     /locus_tag="ACHJWB_000933"
     CDS             complement(12183..12530)
                     /gene="yidH"
                     /locus_tag="ACHJWB_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4312612.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12701..13915)
                     /locus_tag="ACHJWB_000934"
     CDS             complement(12701..13915)
                     /locus_tag="ACHJWB_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4312613.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSSGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSLQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKCKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILGQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNVNGARNILAAGHAVLACGEMVQSGRPL
                     KQEPTEMIQATA"
     gene            13923..14348
                     /gene="tnpA"
                     /locus_tag="ACHJWB_000935"
                     /pseudo
     CDS             13923..14348
                     /gene="tnpA"
                     /locus_tag="ACHJWB_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            14414..14488
                     /locus_tag="ACHJWB_000936"
                     /pseudo
     CDS             14414..14488
                     /locus_tag="ACHJWB_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14485..15807)
                     /locus_tag="ACHJWB_000937"
     CDS             complement(14485..15807)
                     /locus_tag="ACHJWB_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO4312614.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPLQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(15807..17423)
                     /locus_tag="ACHJWB_000938"
     CDS             complement(15807..17423)
                     /locus_tag="ACHJWB_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4312615.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            17719..18435
                     /gene="yidP"
                     /locus_tag="ACHJWB_000939"
                     /pseudo
     CDS             17719..18435
                     /gene="yidP"
                     /locus_tag="ACHJWB_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(18432..20093)
                     /gene="yidE"
                     /locus_tag="ACHJWB_000940"
     CDS             complement(18432..20093)
                     /gene="yidE"
                     /locus_tag="ACHJWB_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4312616.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(20289..20717)
                     /gene="ibpB"
                     /locus_tag="ACHJWB_000941"
     CDS             complement(20289..20717)
                     /gene="ibpB"
                     /locus_tag="ACHJWB_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4312617.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(20829..21242)
                     /gene="ibpA"
                     /locus_tag="ACHJWB_000942"
     CDS             complement(20829..21242)
                     /gene="ibpA"
                     /locus_tag="ACHJWB_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4312618.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(21236..21308)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            21548..21880
                     /gene="yidQ"
                     /locus_tag="ACHJWB_000943"
     CDS             21548..21880
                     /gene="yidQ"
                     /locus_tag="ACHJWB_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4312619.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(21882..23096)
                     /gene="yidR"
                     /locus_tag="ACHJWB_000944"
     CDS             complement(21882..23096)
                     /gene="yidR"
                     /locus_tag="ACHJWB_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4312620.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            23197..24261
                     /gene="cbrA"
                     /locus_tag="ACHJWB_000945"
     CDS             23197..24261
                     /gene="cbrA"
                     /locus_tag="ACHJWB_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4312621.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(24258..25595)
                     /gene="dgoT"
                     /locus_tag="ACHJWB_000946"
     CDS             complement(24258..25595)
                     /gene="dgoT"
                     /locus_tag="ACHJWB_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312622.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(25670..26818)
                     /gene="dgoD"
                     /locus_tag="ACHJWB_000947"
     CDS             complement(25670..26818)
                     /gene="dgoD"
                     /locus_tag="ACHJWB_000947"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO4312623.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(26815..27432)
                     /gene="dgoA"
                     /locus_tag="ACHJWB_000948"
     CDS             complement(26815..27432)
                     /gene="dgoA"
                     /locus_tag="ACHJWB_000948"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO4312624.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(27416..28294)
                     /gene="dgoK"
                     /locus_tag="ACHJWB_000949"
     CDS             complement(27416..28294)
                     /gene="dgoK"
                     /locus_tag="ACHJWB_000949"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO4312625.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(28291..28980)
                     /gene="dgoR"
                     /locus_tag="ACHJWB_000950"
     CDS             complement(28291..28980)
                     /gene="dgoR"
                     /locus_tag="ACHJWB_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO4312626.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            29258..29914
                     /gene="yidX"
                     /locus_tag="ACHJWB_000951"
     CDS             29258..29914
                     /gene="yidX"
                     /locus_tag="ACHJWB_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4312627.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPSGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(29960..30772)
                     /gene="yidA"
                     /locus_tag="ACHJWB_000952"
     CDS             complement(29960..30772)
                     /gene="yidA"
                     /locus_tag="ACHJWB_000952"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4312628.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(30887..31285)
                     /gene="yidB"
                     /locus_tag="ACHJWB_000953"
     CDS             complement(30887..31285)
                     /gene="yidB"
                     /locus_tag="ACHJWB_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4312629.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(31525..33939)
                     /gene="gyrB"
                     /locus_tag="ACHJWB_000954"
     CDS             complement(31525..33939)
                     /gene="gyrB"
                     /locus_tag="ACHJWB_000954"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4312630.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(33968..35041)
                     /gene="recF"
                     /locus_tag="ACHJWB_000955"
     CDS             complement(33968..35041)
                     /gene="recF"
                     /locus_tag="ACHJWB_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4312631.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(35041..36141)
                     /gene="dnaN"
                     /locus_tag="ACHJWB_000956"
     CDS             complement(35041..36141)
                     /gene="dnaN"
                     /locus_tag="ACHJWB_000956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4312632.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLHVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(36146..37549)
                     /gene="dnaA"
                     /locus_tag="ACHJWB_000957"
     CDS             complement(36146..37549)
                     /gene="dnaA"
                     /locus_tag="ACHJWB_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4312633.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            37846..37926
                     /locus_tag="ACHJWB_000958"
     CDS             37846..37926
                     /locus_tag="ACHJWB_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312634.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            38156..38296
                     /gene="rpmH"
                     /locus_tag="ACHJWB_000959"
     CDS             38156..38296
                     /gene="rpmH"
                     /locus_tag="ACHJWB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4312635.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            38313..38672
                     /gene="rnpA"
                     /locus_tag="ACHJWB_000960"
     CDS             38313..38672
                     /gene="rnpA"
                     /locus_tag="ACHJWB_000960"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4312636.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            38636..38893
                     /gene="yidD"
                     /locus_tag="ACHJWB_000961"
     CDS             38636..38893
                     /gene="yidD"
                     /locus_tag="ACHJWB_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4312637.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            38896..40542
                     /gene="yidC"
                     /locus_tag="ACHJWB_000962"
     CDS             38896..40542
                     /gene="yidC"
                     /locus_tag="ACHJWB_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4312638.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            40648..42012
                     /gene="mnmE"
                     /locus_tag="ACHJWB_000963"
     CDS             40648..42012
                     /gene="mnmE"
                     /locus_tag="ACHJWB_000963"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4312639.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10091 a        11506 c        10825 g         9770 t
ORIGIN      
        1 tctttatgat aaataataat caaattgata aaatcaaaat gagaaaaata tgaataattc
       61 tattaaccat aaatttcatc acattagccg ggctgaatac caggaattgt tagccgtttc
      121 ccgtggcgac gctgttgccg attatattat tgataatgtc tctattctcg acctgatcaa
      181 tggcggagaa atttccggcc caattgtgat taaaggacgt tacattgccg gtgttggcgc
      241 agaatacgct gatgctccgg ctttgcagcg gattgatgct cgcggcgcaa cggcggtgcc
      301 agggtttatt gatgctcacc tgcatattga atccagcatg atgacgccgg tcacttttga
      361 aaccgctacc ctgccgcgcg gcctgacgac cgttatttgc gaccctcatg aaatcgtcaa
      421 cgtgatggga gaagccggat tcgcctggtt tgcccgctgt gccgaacagg caaggcaaaa
      481 ccagtactta caggtcagct cttgcgtacc cgccctggaa ggctgcgatg ttaacggtgc
      541 cagttttacc cttgaacaga tgctcgcctg gcgggaccat ccgcaggtta ccggccttgc
      601 agaaatgatg gactaccctg gcgtaattag cgggcagaat gcgctgctcg ataaactgga
      661 tgcatttcgc cacctgacgc tggacggtca ctgcccgggt ttgggtggta aagaacttaa
      721 cgcctatatt actgcgggta ttgaaaactg ccacgaaagt tatcagctgg aagaaggacg
      781 ccggaaatta caactcggca tgtcgttgat gatccgcgaa gggtccgctg cccgcaatct
      841 caacgcgctg gcaccgttga tcaacgaatt taacagcccg caatgcatgc tctgtaccga
      901 tgaccgtaac ccgtgggaga tcgcccatga aggacacatc gatgccttaa ttcgccgcct
      961 gatcgaacaa cacaatgtgc cgctgcatgt ggcatatcgc gtcgccagct ggtcgacggc
     1021 gcgccacttt ggtctgaatc acctcggctt actggcaccc ggcaagcagg ccgatatcgt
     1081 cctgttgagc gatgcgcgta aggtcacggt gcagcaggta ctggtgaaag gcgagccgat
     1141 tgatgcgcaa accttacagg cggaagagtc ggcgagactg gcacaatccg ctccgccata
     1201 tggcaacacc attgcccgcc agccagtttc cgccagcgac tttgccctgc aatttacgcc
     1261 cggaaaacgc tatcgggtca ttgacgtcat ccataacgaa ttgattacgc actcccactc
     1321 cagcgtctac agcgaaaatg gttttgatcg cgatgatgtg agctttattg ccgtacttga
     1381 gcgttacggg caacggctgg ctccggcttg tggtttgctt ggcggctttg gactgaatga
     1441 aggtgcgctg gctgcgacgg tcagccatga cagccataat attgtggtga tcggtcgcag
     1501 tgccgaagag atggcgctgg cggtcaatca ggtgattcag gatggcggcg ggctgtgcgt
     1561 ggtacgtaac ggccaggtcc aaagtcatct gccgttgccc attgccggcc tgatgagcac
     1621 cgacacggcg cagttgctgg cggaacaaat tgacgccttg aaagccgccg cccgtgaatg
     1681 cggtccgtta cccgatgagc cgtttattca gatggcgttt ctttctctgc cagtgatccc
     1741 cgcgctaaaa ctaaccagtc aggggctatt tgatggcgag aagtttgcct tcactacgct
     1801 ggaagtcacg gaataataaa aaagcccggc gtcatgccgg gcaaaagtca ccagttacgt
     1861 ttatgccact gtcaactgct gaattttttt ctcgcggcgg attttgcgtt cttccataac
     1921 cgccactatc gccatcaggc agatacaacc aatcgcggcg atatcaagtg cggcgaaggt
     1981 gcctgcccag ccggtaaggc cgaataccgg cgtcccatcg gcaatcattc ccagacctaa
     2041 cttggcaaag ctgtcaccaa tcaggtaagc aaaggtgcct ttaataccat cggcagcgcc
     2101 aatcgctttt ttaggtacaa agccaacagc agccacacca atcaacaatt gcgggccaaa
     2161 gaccaggaaa cccaacgcaa agagagaagc cagatagata tattcgttgc tggcatgttg
     2221 atacacaccg agcgtggcga taatcagcgc cagcgcgatg caggccacca ggccacggcg
     2281 accgttcgcc aggtcagaga gccagcccca cagcagcgta ccgaccagcg caccagcttc
     2341 aaacagcgta aagccccgaa tcgccaccgc tttagagagt ttcagttcct ggaacgcgta
     2401 tacggttgac cactggtcga taccaatacg taccacatag aggaaaatgt tggcgaagca
     2461 cagcagccag atcactttgt ttttcagcac atactcaaca aagatctgcc acttggtcat
     2521 atcggtagat tctgtctctt tgtcctcttc gctgatctcc tcgccgaaca gttcttcagc
     2581 tttgccgagg ccataagatt ccggggagtc gctgccgtaa cgcaggccga taaaaccgac
     2641 aatcagcgcg ataatcgacg ggaagataaa catgccgatg acatggccat cgaacaggta
     2701 atttgccccg aacagcgcca cacctgctgc gcctgcaccg ccaaggttgt gagaaatatt
     2761 ccagaaaccg aggaatgtcc cgcgtttacg acgcggcgtc catttggtga tggtggagta
     2821 actgcacgaa ccgccggtac tctggaaaaa gccgcttaag gcgtagaagg caatcatcag
     2881 gaacaggcta accgagccgc tgcccatact ggcactgaag cccagcatac aaatggcaga
     2941 caggatcagc atgaacggca ggaattgttt ggtgtttttg ccgtcggcgt agtaggaaac
     3001 cagcgtttta cccacgccat aagtgatgga gaaacccagg ccgatcatcc ccagctgcgt
     3061 catgctcaac ccgtaggtcg agatcatgtc gttctgcgcg atgttaaagt tcttgcgaat
     3121 caggtacatc gtcaggtagc cgataaagac caccaggtag gattgcatga acggtttgaa
     3181 ccacattttg cgccgcactt cgagcggaag gtccagggtc ggcttgcgaa cctggtttaa
     3241 gaaagccagc atgggttact cctgaaatga atacctgcgt gaggcatgca ttgtaaaaat
     3301 cagcagcaaa tcaggcctga gatagcgtcc aggcaaaacc tggaaaattt cttagttttg
     3361 ccggatatga agtgaaaagg tgagatgcat cacgcttcgc gcggtgcctg ggcgttcaaa
     3421 aagggcagta acagcagtgc ggaaatcccg gcggcgatag agataaccac aaaaaatccg
     3481 ctccagtgcc aggtatcgag tactttcgcc agcggccaac cagcaagcga cgcccccaga
     3541 taagcaaaca agccgacaaa ccccgtcgcc gcccctgccg cctctttgtg ggaacactct
     3601 gccgccgcca tgccgattaa catctgcggg ccaaagacaa aaaaaccaat ggtgaagaag
     3661 caggttgcct gcatcacgta gctggcaaat ggcatcagcc acagcgagcc gactgaaagc
     3721 aaaattccgg cggcgaaaat caaattcatc ggcccacggt tgccgttaaa caatttgtcc
     3781 gagccccaac cggctaccag cgcaccgata aatccgccca gttcaaacat cgtcactgcc
     3841 gtattcgccg tgaccagatc gacgcccagt gtctcggaca tatacaaatt gccccagtcg
     3901 ttgatcgccg cccggaccac atagaccagc acatagcaaa acgaaagcag ccagatatac
     3961 ggattcagca acacatattt ggtgaggatc tctttacgcg tcaaccctgc cccttcttgt
     4021 tgttgagcaa tttccagcgc gtcgtgtcgc cattcaccga ccgccggtaa acctaacgcc
     4081 tgcgggcgat cgcgtagccg ccagcagaga aaaatcccca cgactatcgc catacaacca
     4141 gcaatcatca tcccggcacg ccagccgtaa tgcagcgcag ccgctgccat cacaatggga
     4201 atgagtgcgc cgccgacgtt atgcgccgtg ttccataatg cccaccaacc gccgcgctcg
     4261 gtacgtgaat accaggccgt taacagacgc gcacacaccg gtgaacccca gccctggaaa
     4321 aaggcgttca gcacccagag cacggcaaac gcccatagcg acgtcgagaa gccaaacaga
     4381 atgttgatga tgcccgtggc gataagccct atccccataa aataacgggc atttgagcga
     4441 tcgctgacaa tgccggagac aaacttcgac acgccgtagg taatgtaaaa cagggtcgct
     4501 aacaggccga tatcgctacg gctgagcacg ccgttagcaa ggatttctgg tacggcggcg
     4561 ttaaaacttt tacgcgtgaa gtaaaacagc gcgtaaccca gccagatggt cagcagaata
     4621 tgccgacgcc agtagcgata gcgggcatca atttcatgtt tgtcagtcat taatggcgca
     4681 tcggcaggcg ctttcagaaa cggcaacatc atcgaacctt agacatagcg ttgaggtaga
     4741 gaaacgctga cacgcgtgcc gtgcagacag gaaatgtgta atgtgccgcc cagcgccgtt
     4801 acgcgctcgc gcattccggt gaggccaaaa ccttgttgcc cggaacccgg cggcaaaccg
     4861 ctgccatcgt cttcaatcac cagcatcaac cgttcatcct gctgccagcc ttgcagcgtg
     4921 accgcgctgg catcagcatg tttcacaatg ttgttcagcc cttcctggca gacacgaaac
     4981 agcgtcacgc gctggttttc acttaatgct gattcatcga ttcgccattc gagatggctg
     5041 acaatgccgc gcccttccag ctccatttcc cgcatcagtg agcggatggc ctgctccagg
     5101 gagagatcat ccaactggcg cggacgtaac cgtcctaata aacggcgcac cgcgtcgtac
     5161 acgcccagcg acagttgttc gatgagctgc ccgctctgct tcacgctggc gttatctgcc
     5221 gccagccgct gaacaatgcc cgcctgagta cgaatggctg tgatggtctg accgatatca
     5281 tcatgcagct cacgcgccac atcacggcgc acgctctctt cggtttccag caaccgttca
     5341 gccagatgct gattgcgcgc cagttccttt tgcagcgact ggttaagttc acgcaaccgc
     5401 tggatgccag cgccaagcaa caaccctgtc agactttgca ccagcagcga gagcaataaa
     5461 tccaccggat gatcgcgcca ggtttgactg gcgatcagcg cgatggcgtt catcaacgtc
     5521 gcaatcagcg ccccttgcca accatagtgc caggccagcg cgataatcgg cagcgccaga
     5581 caaaatggcg taaagcgcga cagttcgtcc ggcaatccca actggagcca gagactgata
     5641 acaaacagca gcaagtacca gaccagatgt cgcccgcgcc agttgatcgg ctgagaaacc
     5701 agtgacgggc cgagcggcag ccaggtgtta ttggcgagat agtgccagaa caccagacat
     5761 atcggggcca gcgtcaggcc gccagttaaa gtcagcaaca gcgcattcca cgactctttg
     5821 ccgtgccaaa gccagggcag cgactgcaac aacgccgccg ccgttaacgc cgcaccctgt
     5881 aacagcaagg tgcgccagtc acgctgatgg cgatagcgcg agatcagcgc cacgggcagt
     5941 aacgtcagta aactaccgat catcaataac ggaaaatggg ttaaaccgac cgcctgcgtt
     6001 agccagtaaa tcagcagcca ctccgcgccc agcagcaccg gccagtatcc gcgcgggcat
     6061 tgcagcatta gccccagacg cagaccaaac ggaaataaca gcaccgccat atcagggcgc
     6121 tcaaccaggt gcaggctgat actccacagg caaaaccatg cggcagagaa gataaaaaag
     6181 caggcaataa cggtaattaa gcgggagaac aacgtcttca tcaccagcca tcaaacatgc
     6241 ggcgcgccag ctctacgtcg ttactgacac ccagtttttc catcaggttg gcgcgatgga
     6301 cgtgcaccgt tttcggcgac aagcccagtt cggcagcaat ctctttcacc gccattcctt
     6361 gcgccagttt ttccgccacc tgccgttcgc gtttggttag cgggtcctga cgaccggatg
     6421 ccagtttaat ggcaatatcc ggcgtcagat aacagccgcc cgtggcaacc gtatgcaccg
     6481 ccgcaatcag ttcgtcaggg ctacagcgtt tggagagaaa gccgcgtgcc cccgcgttaa
     6541 gcgcctgctc aaccagcgcc ggactgtcat gaacagagag cattattgtc gccatacctt
     6601 tcggcagctg gcttagcagt tccagaccgg agatatcggg catggagata tcgcaaatac
     6661 acacctgcac accgcgcccc ggcagccccg ccagcgcctc gcgccccgaa ccaaactcgg
     6721 caactacctg caaatcaggt tccagcccca gcagctgcgc aaagccggag cggacgatga
     6781 ggtgatcgtc tataagggca acggtgatca tggtcttgtc ctggcgggta aaaaaaaacg
     6841 cgcttacctt aacgataagc gcgatgttgt tcaagccttg agcggttact gaaaaaacac
     6901 cgcaatcttg ttaaacatag tcggatcgga ctgattacgc tgcactttca ccacatcttc
     6961 cagcttatcg atttggctta tcatctgctc cagacgctgg tcgtcattga ccagtagcca
     7021 gatatggctt ttgtcgctgt cctgaatcgg cagacaaaga atgccttcaa cgttaaaagc
     7081 gcggcgggca aaaaggccac aaacgtgggt cattacgccc ggatggttgc gaacggtgag
     7141 ctccagaatc acgttgtcat gagttgtgtt ttgcatggct tattccccca ccatttcagt
     7201 attcgccgca cctggcggca ccatcgggta aactttttct tcggcatcaa tgcgcacatg
     7261 gatcagcgcc gggccagggc gattgatgat ttcctgcaat gcagcctgcg gatcggcttc
     7321 gttattcaaa tcacaggttt cgaggccgaa tccggcggca atctgcataa agttgatttt
     7381 tcccggatag gtggcggcaa aaacgccttg ctcgtagaac agactctgtt gctgatgcac
     7441 cagccccagc gcttcgttgt tcatcagaat gattttgaca tccagctgat tttcactggc
     7501 agtcgccatc tcctgaatat tcatcatcag gctgccgtcg ccggagaaac acaacacttt
     7561 gcgatccggg ttcgccagcg ccgcgccaat cgccgcaggc aggccaaaac ccatcgtgcc
     7621 cagcccaccg gaggtcagcc actggcgtgg gcgattgagc ggataagctt gcgcggtcca
     7681 catctgatgc tgacccacat cggtggtgat aatcgcattg tcatcgacac aggcggcaac
     7741 ggcgttgatc aggccgtaat ggcttaatgg atcgcacgct ttcgggattg gacacgggaa
     7801 ttcgcgctgc aaatccgcta ccaactggtg ccactctgca cgcggttgcg cttccacctg
     7861 cgggatcaat tgcgccagca cgtcatcaac atccgcctga atcgccacat gcggctgctt
     7921 gattttaccc agctctgcgc gatcgatatc gacatgaata attttggcat tcggacagaa
     7981 ctgctcggtt ttgccaatcg cccggtcatc aaaacgcgca ccgagcacta tcaacaaatc
     8041 cgcctcctgc aaaatatagt tggtgctgcg cacgccgtgc atccccagca tacccagcga
     8101 caacggatgc gcttttggca acatgcccag cgccattaaa gtcatggtgg taggcagttg
     8161 cgctttctcc gccagttcac gcacccgtgc gggcgcattg atcacaccgc cgcccagata
     8221 aagcaccggg cgtttggcag cgttaatcat cgccgctgcg tcacgaatgc tttcttcgct
     8281 aaaggcgggg gcggcggctt tttctgccat agcgggctgt gtttcaatct caaaaactgc
     8341 cgtttgcaca tccttaggaa tgtctatcca caccgggcct gggcggcctg attgcgcaat
     8401 gcggaaggca tcgctcatga cctgcgggag ttcttcgata tgtctgacca gatagttgtg
     8461 tttggtgatg gggatagaga tgccgtaggt gtccacttcc tggaaggcgt cggtgccgat
     8521 catcgaggcg ggaacctgac cggtgatgca aatcagcggg atggagtcca gccgcgcatc
     8581 ggcaatggcg gtcaccaggt tagtcgcacc cggtccgcta caggccatac agaccgccgg
     8641 tttaccgtcg gtgcgcgcca tcccctgagc gataaatccc gcgccctgtt catgacgggc
     8701 cagaatatgg cggatttgcg tgctttggct taaggcatcg taaacaggca ggatagaacc
     8761 gcccggaatg cccgtcacaa tcttaatgcc ctgctgttcc aggaaatgaa cgataaattc
     8821 tgcgccggta aagcgcttac gcgtcgatgt tgtgcccgaa cttgccatgc tccagtcctt
     8881 ttcttctggg ccgactttcc gggaggtgct taaacgaaaa accccgcccg gtttgcgccg
     8941 gcggggtttt ggaatcgtgt gttgttccag tccctacggc gcattgccga cgaccaccac
     9001 cacacgcacg acgaccactg cggcggatag cgcagttggt agtagttttg cgttgaacat
     9061 ggaagtagtc attggggacc ttgttggttt tgtgtttaac aatatttata caagcacagc
     9121 tttacagggg agacaatgga aaatttttca gcaagggaaa attgaggggc tgatcacgtt
     9181 ttgtactgaa ttgcaggtaa caaaaaaccc cgccggagcg aggtttcatc agccgcctgc
     9241 ggctggtaac cgcaaagcac actgtattat gtcaacactg aaagtatacg tgttccgcgc
     9301 agaacgcgca atttcggcac gaattttgac gtatttagtg catagttgag tatcgatcac
     9361 agtttgcatt ttgtccaaat attactgttt atttatacag taaacttcta taatatcact
     9421 gtacgcaatg tgttatgcgg gggccgcatc gttacccggc gcactaagtc ctggctgaaa
     9481 cgggtggtgc cgtcagcgcc ttaaccccgc gtgagcacac tgtgttatgt caacaagcac
     9541 aacgtttctc cttgagatac cgcgtgcaca acagctggca acaggcagcg gaaaggtacg
     9601 tcagctggca gtgctcctga accacaggag acgcgtatga gcctagtgga tatcgccatt
     9661 cttatcctca aactcattgt tgcagcactg caactgcttg atgctgttct gaaatacctg
     9721 aagtaattca gattcaagtc gcaccaaagg ggagcgggta accgctcccc ttttatattt
     9781 agcgtgcggg ttggagttgg gggcttcttc taccagcaga atgtttgcac ctgcgctgcc
     9841 tgtgcccctc accctagccc tctccccaaa ggggcgaggg gaccgtcccg tgcaatgttt
     9901 agctcatttt ctgaaaggga acggtggttt ccccctcgcc cctttgggga gagggttaca
     9961 gttcgcacgt aatccctccc ggcaacattt attgccgctt ttgtttacat attctgccgc
    10021 taaacaattc cccattcctg gcgtatatct ggctaacatt catcaatgtg atagattcct
    10081 ctcccgcatt tatgggaatg cgtagtgact tattctaatt atttttataa aagcatccgt
    10141 gataatgaaa aggcaaagaa acgtcaattt gttattgatg ttggtattac tcgtggccgt
    10201 cggtcagatg gcgcaaacca tttatattcc agctattgcc gatatggcgc gcgatctcaa
    10261 cgtccgtgaa ggggcggtgc agagcgtaat gggcgcttat ctgctgactt acggtgtctc
    10321 acagctgttt tatggcccga tttccgaccg tgtgggtcgc cgaccggtga tcctcgtcgg
    10381 aatgtccatt tttatgctgg caacgctggt cgcggtcacg acctccagtt tgacggtgtt
    10441 gattgccgcc agcgcgatgc aggggatggg caccggcgtt ggcggcgtaa tggcgcgtac
    10501 tttgccgcga gatttatatg aacggacaca gttgtgccat gctaacagcc tgttaaacat
    10561 ggggattctc gtcagtccgt tgctcgcacc gctaatcggc ggtctgctgg atacgatgtg
    10621 gaactggcgc gcctgttatc tctttttgtt ggttctttgt gctggtgtga ccttcagtat
    10681 ggcccgctgg atgccggaaa cgcgtccggt cgatgcaccg cgcacgcgcc tgcttaccag
    10741 ttataaaacg cttttcggta acagcggttt taactgttat ttgctgatgc tgattggcgg
    10801 tctggccggg attgccgcct ttgaagcctg ctccggcgtg ctgatgggcg cggtgttagg
    10861 gctgagcagt atgacggtca gtattttgtt tattctgccg attccggcag cgttttttgg
    10921 cgcatggttt gccggacgtc ccaataaacg cttctccacg ttaatgtggc agtcggttat
    10981 ctgctgcctg ctggctggct tgctgatgtg gatccccgac tggtttggcg tgatgaatgt
    11041 ctggacgctg ctcgttcccg ccgcgctgtt ctttttcggt gccgggatgc tgtttccgct
    11101 ggcgaccagc ggcgcgatgg agccgttccc cttcctggcg ggcacggctg gcgcgctggt
    11161 cggcggtctg caaaacattg gttccggcgt gctggcgtcg ctctctgcga tgttgccgca
    11221 aaccggtcag ggcagcctgg ggttgttgat gaccttaatg ggattgttga tcgtgctgtg
    11281 ctggctgccg ctggcgacgc ggatgtcgca tcaggggcag cccgtttaag cgcacgtcac
    11341 cgcagcatcg tcatcagctc catgggagaa cgatgctgct ttatcagatc acgcatcacc
    11401 cgcatatgcg gtgcggagta agaataaaac gcctgatagc ccgcacaaag cacgctgtct
    11461 ggcgtctcct gccgagcgtg cgggcacagg cgatgccagt cgcacgcctg gcagtcacgg
    11521 cgcgattgcg ggttttcgct gccacaccat ttttgtaggg tggagtcaaa aagctcaatc
    11581 gagatacgac tgatatctgc ccgcacccaa cactcgaaca gggcaattaa aaatttcccc
    11641 cacagctctg ctgtcaccga agcggccgtc atatcgcccc ggtcatcaaa ttcaagatgg
    11701 ggaatgagct gtacggagcg gatgccccgt tgtttgaaat tgtgataaaa atcggcaggt
    11761 tgcagagcat ccttgtactc tacaaccagt ttatgcctgt cctcttcagc atcaatgacc
    11821 tgacttcctg tcatgcgact ctctcaataa atacgataag ttgatgtata tgcgttacag
    11881 caaacagtat tgcgagagat aacaccgcga gggagatcaa aaatttgtca cggactacgt
    11941 gaaattggga aaaatcgcta ttcgtgacat ccattaaatt gcgattacgg gtgtagtgcc
    12001 agaggatcag cgccacgatg gcgaggatgc caatcgaaat ccagaataac atgcccgcct
    12061 ggtgccagtt gtgtttgata gccaacgcca tcaacgcgcc gtagcccagc atggtacgaa
    12121 accacgccag tgatgtgcgc tccggctgca gtcctgggtc ggcaatgcgt ctggctttgc
    12181 ggctatccgg catacaacac cagtcccata acgatcaccg ccacgaccat caatattaag
    12241 ctaatgatta ataagctgtt ggtgtagggc aggtcctctt tcagacgcat cgctttttca
    12301 ttgcgtaacc agcgcaggta gccatacatt gctaacccgc cggaaaacag acacaacaac
    12361 aacgccagca gttcacgaat gacgggcgtg gcgaaatctg gcgcaagttg atcaagccca
    12421 acccccgccg ccagaaatcc tagtgcggta cggatccacg ccagaaaagt acgctcatta
    12481 gccagcgaga agcggtaatc gggcgcttct ccgaggcggg aaattttcat tgttgctcct
    12541 ttgcaatttc actaacgctc tatctttagc ggtttttttt aagaatttcg tccgcccgtt
    12601 tcctgatatg cttttgccat ccgctacaag gaggtcagat gggtatcata gcgcaaattg
    12661 acatcctcca cgccctgaag gacgtggatc cctgctacgt tcaggctgtc gcctgaatca
    12721 tttcggtggg ttcctgcttc aacgggcggc ctgactgcac catctctcca caggcaagaa
    12781 cggcgtgccc cgccgctaaa atgttacgag cgccatttac atttacatcg gcgttcgctg
    12841 tatatccaca tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat
    12901 gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg aacagcaagc acctgaccgc
    12961 cactccagag ctgcttgtac tcaagctggc ggcgcatttc ataccagccc tgacccagta
    13021 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
    13081 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
    13141 tgctgacggt cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacgggaat
    13201 gcagtcgctg tattttgcat ttctgcttct gccagttgtt gctgaatttg accttgcggc
    13261 ttaactgccg ctgaagtctc gccagcttct tctggttttt ctgcaaactg tttacaggct
    13321 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
    13381 ttgatgctga agggtgaacc ggagtggata cttcactttc tgtctgaata ctgatgtacc
    13441 acttaccgct ggactggctg accgtgacat ttttcacaac tcccgtgacc tgacggctat
    13501 tccggtagcg catccagcca agtttcggca gaaaaatacg gctgttttcc tgatcgagct
    13561 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
    13621 cagcccgatt ctggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
    13681 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
    13741 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
    13801 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
    13861 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggcc
    13921 aaatgatact ttggtctatg aataaagaaa ccgatatccg acggggaagg cattgtgttt
    13981 tcctgatgca tgttcacctg gtctttgtca ccagataccg acgtcagatt tttgaccatg
    14041 acgcgacaga aaaactacgc gcttactttt caaatgtatg tgctgatttt gaagctgaac
    14101 tggttgaaat ggatggcgaa ccagatcacg tccatttgtt aattaactat cctcccaaac
    14161 tggcgatatc cagtctggta aacagcctca aaggcgtatc gggtaggtga ctgcgacgag
    14221 atcgaccaga tattgcagtc aggtattact acaaaggcgt tttgtggagt cctggctatt
    14281 ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacgtt gaacaacagc
    14341 aaacacctgg tcaggtggaa aaccgcgcct tatatccccg ccctgaagga cggggtttta
    14401 cggcgcaccg gataaatttg gttatgcgcc gtcagccgtg ctaaagaata cggaccagca
    14461 cccaacggat gccagtccac acaattaacg taactctggc cagtacgctt tgttggcctc
    14521 cagcaactca tccagaatag ctttggcgac cgaggcgctc ggtaccgttt tcgataacgt
    14581 aatcgcctgc cacaggtgct gatatgaacg ctgttcccag gcgtccacga ccagtttttc
    14641 caccgccact tgctgactca ttaacccttt ctgaaactgc gggatatcgc cgaccaccag
    14701 tggttctggt ccgttgtgcc cgaccagaca cgggatttcg accatcgctt catcatcaaa
    14761 gttatgaatt gccccgttgt taggcacaat cagcaacatc cgctcctgag tgttgaaggc
    14821 aattgccgcc gccagatcga cgatgtatga cgcatgttcg tcaatttcca gcttgcccgc
    14881 cgctgaattt cctgccgccg taatggcgcg gcaagcatcg aaaacctgtt tctcgcgatg
    14941 ttccatcacc tcattcgcgc gggtatgttc agggttggaa tgctgaacga cataatccgg
    15001 gaagagataa tatttcagat aggtgtttgg taatgtatcc gggtccagtg cctggacatc
    15061 ccgcgctttt gcataggtgt cgttccagct cgcttcagta tgtgtatctt gcagcggtgg
    15121 aacataaccg tgcttagaga catattgccg cagctggggc atcaggtcgt tgccctgcaa
    15181 atcttcaatt gatgtccacc agccaaagtg gttcaggccg tagtagcgca cgcgcatctg
    15241 tttgcgatct tgcagcccaa caatttgcgc catccggctt tcaataccga ttggcatgtc
    15301 acagatgttg aggattttcg cattcgggcg cagacgacgc gtggcttctg cgacaatggc
    15361 tgccgggttg gagtagttga gcatccaggc atttggtgaa tatttttcca tataatccac
    15421 cagttccagg acgccgccaa tggaacgcat gccgtacgct attccgccgg gtccgcaagt
    15481 ttcctgacca acaacaccgt ggcgcagtgg gattttttca tccagttcgc gcatcgggta
    15541 cttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg ctgaatgcca cttcaggatc
    15601 ggtggtgtaa ctaaacgcaa tgtccggcgc tttctcttta aggatgactt tacaggcttc
    15661 ggcaatcact tcctgccgcg caccatcgtt atcataaaat ttcagtgcac gaagcgggaa
    15721 acggtcctga ttcgccagga gcatcaacac gatgcctggc gtaaaggtgc ttccaccgcc
    15781 tgcgacaacc actgagaatt tggtcattat actgcctccg taatggcaac attttctgct
    15841 gattgatgag aattaattaa gctatcgagc tgttcacgca gctgggatac atgcagacca
    15901 atgatcacct gaatggcatc gccactacgg aagacgccgt gcgctcccag ctttttaaag
    15961 acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa tacgtaaacg cgtcgcgcaa
    16021 ttgttaatgc tggagatatt gccgaccccg cccagggctt gcaggatacc ggcagcctga
    16081 tccagctctt tttttggctc tgccgcggtg gtttggcctc gcgaggcttt gtattcggct
    16141 tttgagtaga gtttcacttc cgcatcttca cgtcccggcg tgcacatgtt gaactgcaga
    16201 atcagtgtgc ggaaaaccac gaagtacagc agggtaaagc acaacccaat ggcgatttgg
    16261 gtcagcatca tatccgcgtg gttgctgaac atcgggatcc agttttgcgg taaaacctgg
    16321 tcaatcagac ctccgcccat gttgcccacc acaccaaaga gatacattac ggtcgacatt
    16381 gaggccgcca gcacggcgtg taccgcaaac agcaacggtg aaatgaacag gaaggtaaat
    16441 tccagcggtt cggtaattcc caccagcatg gcggttaagg ttgcgggaat cagcaagccc
    16501 gccactttta cccgattttc cggtgcggca gtgaagtaca tcgctaaaga aatgcccacg
    16561 gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc cttccgggaa caacgatttc
    16621 agcggctcgg cgctcaaact gaactcttgc agatgctgcg cccagtacat ctgaatgccg
    16681 ccttcaacag ctgccggacc aaagataaac tgtccgtaga tgaagtggtg taaaccggtt
    16741 gggatcagaa tacgttcgag gaaggtgtaa acccagaccc caagtgcacc cgccgaacgc
    16801 aggaacgctt gcagagattc aatccccatt tgtacttttg gccagccgag caacgtcagc
    16861 caggcacagg ggatcatcac caggaaggcg ataatcacca cataagacgt cccctggaaa
    16921 atgccgagaa aaaccggcag ttttttatcg aacagacggt tatgcagcgc cgtcacaatg
    16981 ccggaaatga taattgcgcc gataatgctg gtatcgaggg ttttaatccc ggccatcatt
    17041 gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga cgccgaagta gcttccccag
    17101 gtcattccca tcgcgttgat gaaatagttc caggtcagga aactcaccat caccgccaga
    17161 caagcacgcc cctgcgcttg cttagcaagg ccaatgggta aaccgacagc aaaaatcagc
    17221 ggcatattac ggaataccgt ccaaccgccc tcttcaataa tgtgtacgat ttgcgcgaat
    17281 aaactgttcg gatcggtcag tgattccccg acaaacatcg ggttttgcag caagatggca
    17341 agacccacca caatcccggc gaagggaaac agcagcactg gcgtgaacat cgcgccgcca
    17401 aagcgttgaa tttgactgag catttccaca tcctcttttc tcaattctgt aagtaggaag
    17461 cctgattggt tatgcggcag gcctggagcg aggataggaa gatgcgatcg gcagaacatg
    17521 acgacccaat gggattcgtg atcgctttca tgcttttcgt tgggcggtag agatctacgt
    17581 tttcgcgaaa aagtagacat ctgtgagcac tggtacacag catcgaacac atctttaaaa
    17641 aaaagatgtt ttttcaatcg attaagcaga acttgtgggc gcattacccg ggcttgcagg
    17701 caaaaaagag atctagagat gatctacaaa agcattgcgg agcggttaag aattcgactt
    17761 aattccgcgg atttcacgct aaacagcctt cttcccggtg aaaaaaagtt ggcggaagaa
    17821 tttgcggtat cgcggatgac catccgtaaa gccattgacc tgctggtagc gtagggattg
    17881 gtggtccgcc gccacggcag cggcacttac ctggtgcgca aagatgtact gcatcaaacc
    17941 gccagcctga ccgggctggt ggaggtgtta aaacggcagg gaaaaacggt caccagccag
    18001 gtgctgattc ttgaaatcat gcctgcgcct ccggccattg ccagccagtt acggattcaa
    18061 atcaacgagc agatctactt ctcccgtcgc gttcgttttg tggaagggaa accgctgatg
    18121 ctggaagaca gctatatgcc ggtaaaactg ttccgtaacc tttcgctgca acatctggaa
    18181 gggtcgaagt ttgaatatat tgaacaagag tgtgggattt tgattggcgg taattatgaa
    18241 agcctgacgc cggtgctcgc cgatagactg ctggcgcggc aaatgaaggt agcggaacac
    18301 acgccactgc tgcggatcac ctcgttgtca tatagtgaga gcggggagtt tttgaattat
    18361 tcagtgatgt tcagaaatgc cagcgaatac caggtggagt accatttacg gcgactccac
    18421 ccggaaaaga gttaaccgat actccagaag agcaccgcca gtaattgggg ggtgataatg
    18481 cgcaggaaca tcactaacgg atagacagtg gcgtaagaga gcgccgccgc accgctggtt
    18541 ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg tcatggaacc tgccagcatc
    18601 ccgcacatgg tcaggtaatt cattttggct aacatccgcg ccagaatgcc aacagtaatc
    18661 agcggaacgg cggtgatcag ggctccataa ccaatccagc ttagcccttc gccattgacc
    18721 agggtattta caaaatcccc accagatttc agaccaacga ccgagaggaa cagcacgatc
    18781 cccagctccc gcagcgcgag gttggcactt ggcggcataa accagtacag cttgccgata
    18841 ctgccgatac gcccgaggat caacgccata atcagcggac cgcccgccag ccccagtttc
    18901 aacgcggccg ggaatcctgg cacaaagacg ggaatagaac ctaacaatac gccaagcccg
    18961 atgccaataa acactggcag catctgaacc tgttgcagtt tttgctgcgc attccccagc
    19021 acattggcaa cggcatcaat tgcggacgga cgccccacca ggttgaggat atcgccgaac
    19081 tgcaggctga tatcgccact ggcgaccagt tcgaccccgg cacggttcag gcgcgagata
    19141 acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac gttttccgag cacgttttca
    19201 ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt tcgtggacag cgaagtatcg
    19261 acctcctgac caatcaccag ttgcgcatta tgtaaatccg ctggctgccc caccagatgc
    19321 agcaaatcgc ccagttggat aatggtatct ggcgaaggaa cttttagggt ttcttcgcgt
    19381 ttcagacgcg agcagataat tttgtcgccg ttgagaatcg gtacatcttt aatggctaaa
    19441 tcatgcaggt tagggttctc aacgcgaata ttgatagtct tgatcagcgc gccgccattg
    19501 gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat tgacgcggaa aataacccgc
    19561 aacatccaca tggtgaacaa aatcccgcaa atgccgaatg gatacgccat cgcgtaactc
    19621 atccccatct gatcgaccat ttccattggt gtacccaggt cgcgcaaaat ctgctgccct
    19681 gcccccagcg ctggcgtatt ggtaaccgca ccggagaaaa tccccagcac taccggcagt
    19741 ggaatatcaa acagtttatg caggatggcg gtaaccagac caccgatgat gacgatcaga
    19801 acagcaaaca ggttgaggcg taatccggag acacgcaatg aggcaaagaa gcccggccct
    19861 acctgaatcc cgatggtata aacgaacagg atcaggccaa attcctgaat aacatgcagc
    19921 atatcgctac ttaatgtcat ccccgcttga gaaacaaaat ggccgacgat gatcccacca
    19981 aacagcacgc cgccaatacc taatcctatg ccgcgaaatt tgacgttgcc gataaacaaa
    20041 ccgacgactg ccaccaaagc cagaatactg accgttaatg ctatatcact catcgtccat
    20101 ttctcttgag ataacatttt aattgctacg gattatggct gaatcggtga agcagatttg
    20161 ccttatagcg cacattatca cgggaaagaa cgtgccgaaa tatcttaaac agtcgaaatt
    20221 taacgcattg ttgagtaagt aagacaaaaa aagccccacc agaatggcgg ggcaaagaga
    20281 atagctagtt agctatttaa cgcgggacgt tcgctgatag cgatacgctg cgctgcgatg
    20341 ggttcaggct cattacgaat taaatcaata tgcagtaaac cgtttacgaa ggttgcgcca
    20401 gagacttcca tattttcagc cagcgtaaag ctcaggctaa atggctgatt cataagccct
    20461 tgatgcagcc attttttctc ttcttttggc tgctccggcg tgccttttac gctcaggcgc
    20521 gtaccttcca gttgaatctc taaatcttcc tgacggaaac ctgccagcgc aagggtaatg
    20581 cggtagtggt tatcgtcgct tttctcaatg ttgtacggcg ggaagctctg gctttcaccg
    20641 gcgttttgca gtgcgttggc cagtttgtca aaaccgatcc attgacgcat cagtggggat
    20701 aaatcgaagt tacgcatagt catttctcct tctaagaagc gagtaagtac ctgcaaatcc
    20761 gaagattcgc atatgctccc tgacggcgag catggagatg tcaggccgcg ccaggcggcc
    20821 ttagggaatt agttgatttc gatacggcgc ggttttttcg cttccggaat cacgcgttcg
    20881 agatcgatat acagcaaacc atttaccagg ttagcgccac gaacatgaat gttctcagct
    20941 aactggaatt tgcgttcaaa gttgcgttca gcgatgccct ggtacagata ggtgcgctct
    21001 ttttgttcgt cggcgtgagc acctttcacc accagcagat tatcctgggc ggtaatttcc
    21061 agttcgctct cagcaaaacc agccacagcg atagcaatgc ggtaatggtt ttcgtctacc
    21121 agttcaacgt tatacggagg gtagccgcca ttactctggc tctggttgtt ttctaagtgg
    21181 ttaaacaaac ggtcaaatcc aatagcagaa cggtaaagcg gggataaatc aaagttacgc
    21241 ataatcaata gctcctgaaa tcagcgagaa tgtaagacct tccacaatgg acaggtcagg
    21301 tagccagaac acccatcagg cgcgtcctca tcggctacga tgtaaaaatg ggtctggaaa
    21361 tgaacttttc aagccttatc agcggacttt tttttgcagt ttatggtcta ttgcatagac
    21421 tgagggggca gcacgaaacg ttaaagtgcg atagccgcca cagtgcgacg taatgatgat
    21481 gttattttca gcaagggatc gctataactc atcaggccaa cacccacgat caacagatga
    21541 atttattatg ataagaaatg tgttgttagc gttcatgata tgcagcggaa tgacattact
    21601 cggaggatgc tccagcgtga tgtcccatac cggagggaaa gaaggaacgt atcccggcac
    21661 ccgcgccagc gcaaccatga ttggcgacga tgaaaccaac tggggcacta agtcgctggc
    21721 gattctcgat atgccgttta ccgctgtgat ggacacgctt ttactgccgt gggatgtgtt
    21781 ccgcaaagac agctcggtgc gctcacgcgt tgaaaaaagc gaagccaacg cgcaagccac
    21841 caatgcggtt attccaccgg ccagaatgcc tgataactga tttagcgatc agtttcggtg
    21901 atccacagct ggccaccttc catccacgcc agccattgac catccggcga gaagaccacg
    21961 gcgtccgcag aaggtggatt ggcgtggttt tcggttaaat actcaacctc gccgctttgc
    22021 gcatgggcac aagcaattcg attatccagc acaaagccca accattctcc tgacggatgc
    22081 cagttaaatg cagactgaat atccgttttg ttatgggtta actggcgcgg ctcgccgccc
    22141 tgtggcgaga taagccacag ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt
    22201 tgcgtaccct gcggattaca gcgcacccag tggcgcggga cgttgactaa ccccggataa
    22261 gcccgatggt gggtaaaggt taaacgtcgc tgcacgacgc cgcgcggtgg cgcgggcagg
    22321 gtggtttccg ttccacttaa cggcgcatca cctgccgctt tccagccagc ttcatcttgc
    22381 ggtaactcaa cgataaacag ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca
    22441 ataaacgcca gcgcgtgatt tcctacccat ccttcttcat aagcacgatt gatttcatcg
    22501 ctgccaggct gcggcgtggg cgtggtttta ctcaccagca cgcaccagtg gctaccgctg
    22561 tattcacgcg gatgctgctt ttgtacgttg accgggccaa acggcgcagc aacaccgacg
    22621 tttcgcaaat ccagcgccgg atcgagttca tgcattacat ggtcgttata ggtaaagctc
    22681 accctttcac cgttcgggct aaagacatgc acatggctgc cgccgcgcag cgctcctggc
    22741 gtatacggcg cggtaatatc cattgcatcg agattgctca ccttgccgcc ttcagcaatg
    22801 actccgcgtc gatgatggaa gtcgtaatgc catgtttcat caggattttc agggccgtga
    22861 ataaagacat atttctctga cttcggatga acggtcacca cgccgacgtg tgcgccctgt
    22921 gacgcgcgat agataacctc gacctcgccg gtatggatat tcacacgctc aatggtctcg
    22981 ccggtaaacg acgccccaga aggacgcacg tcaaatacca gccactggct gtcgggcgtc
    23041 caagtattgg tattggtgag caggtgatta cggggagcaa aggttatctg tttcatggcg
    23101 cggtcctgat acaaaacggg tcccaccatc atacttcaca aggacttcac tttcagacaa
    23161 tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt
    23221 attggcctcg gcccggcagg atcggcgttg gcgcgaaaat tagcaggcaa aatgcaggtg
    23281 atcgcgctgg ataaaaagca ccagtgtggt actgaaggtt tcagcaaacc ttgcggcggt
    23341 ctgctggcac cggacgcgca gcgatctttt attcgcgatg gactgacgct tcctgtcgat
    23401 gtgatcgcca atccacagat tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg
    23461 cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt gtggatgaaa
    23521 tcgctgatcc ccaccagcgt tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt
    23581 gaggatgata aatggcatgt catttttcgt gcagacggct gggagcaaca tattaccgcc
    23641 cgctatctgg tcggtgccga tggcgcaaac tcgatggtgc ggcgacatct ctacccggat
    23701 catcaaatcc gtaaatatgt cgctatccag cagtggttcg cggagaaaca tccggtgccg
    23761 ttctactcct gcatctttga taattcgata actaactgtt attcatggag tatcagcaaa
    23821 gacggttatt ttatctttgg cggtgcttat ccaatgaaag acggtcagac gcgtttcacg
    23881 acgctgaaag agaaaatgag cgcctttcag ttccagtttg gtaaggcggt gaaaagcgaa
    23941 aaatgcacgg tgctgtttcc ctcacgctgg caggattttg tctgcggtaa ggacaacgcc
    24001 tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg gattagctat
    24061 gcgctggata gcgcagacat tctgcgttcg gtgttactga agcagccaga gaagctcaat
    24121 accgcttact ggcgcgccac ccgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa
    24181 agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg tgtggcacat
    24241 attccacagt tgaaggatta gccaacgcgc ttcacatcgc ccaccagcag gatataagag
    24301 agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggtgcgaa accgtaaccc
    24361 tgcgccaggt accccaccac cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta
    24421 aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagaaac cagcgaccag
    24481 gtaatcgaag caaaaccgtt accgaagaat gccagcgcca tcaggcacat aatcatcatc
    24541 ggatcgttag tgtagttagc gcccataatg caggtggaga tcagcaagcc gcagataatc
    24601 ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc cgctacccag
    24661 ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat aaagcctgct
    24721 ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac
    24781 cacagtgtag aagccaccgc aaattgccca agataaacgc cgatcagttt acgatggaac
    24841 accagtttcc agtctttggc tgttaacggc tgacgcgcct ctttcttcac cggcgcatca
    24901 ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct gatacctttg
    24961 gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat gccgatacca
    25021 ccagtgacaa tgaacaccca gtgccagctc aacatctcct gaatccagat cagcagcgga
    25081 gtcaaaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc ggaagcgcgt
    25141 tcatgttccg ggaaccagct ggtcaccatc cggttattgg ttgggaaagc gggcgcttcg
    25201 aaaataccgg ttatcgcacg cagaccaatt aatgacatta atcccgtggc aaagccctgg
    25261 aataaagtcg ccactgacca accaaatatc gcaataaaat aagtcacgcg agaacctacg
    25321 cgatctaaaa accaaccgcc ggggatctgg catagcgtat aaagccaggc gaaggccgaa
    25381 aatacatagc ccatttccgc tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc
    25441 acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat catcaccagc
    25501 gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat agcgagcttt
    25561 ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca gagggttata
    25621 tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat taccactctg
    25681 ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca tttttactga
    25741 actcaataac tttagcttcg tcgatttcca cacctaagcc cggtttcgtt aacggtttaa
    25801 agaagccgcc aaccatactg aagtcttctt tgtttttcac aaagtcgagt aactccgcgc
    25861 ctttgttgta atgaatgccc atactttgtt cctgaagtac cgcgttatag gaaacaaagt
    25921 cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca
    25981 catcataggc ttctgccatt ccagcaattt tgtagcattc ggtaataccg cctgcatggg
    26041 agagatccgg ttgcagaatc gaaataccgc ctgcctccag cacgcgttta aaatcgaaac
    26101 gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt
    26161 agtattcggc ttgttccgcc agcaccggct cttcaataaa cagcgggcga tacggctcca
    26221 gttctttaat cagcactttc gccattggcg cgctgacgcg accgtggaaa tcaagaccaa
    26281 actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact gccgcatcta
    26341 ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg aaggtatcga
    26401 agccgatttc gcgtagcgtt ttaatgccgt cgataacatc cgccggacga tcgccgccaa
    26461 cccaactgta ggctttaatt ttgtctcgaa ccaggccgcc catcagttgc cagaccggcg
    26521 cattcagcac tttgcctttg atatcccata acgcctggtc gatcccggca atggcgctca
    26581 tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat aagtcattga
    26641 tgcgcgaagg atcctgacca atcaaatagt cacccagctc gtgaactgcc gcttccaccg
    26701 tacgggcgcg gccttcaatc acgggctcgc cccaaccgac cacgccttca tcggtttcaa
    26761 ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg attttcattg
    26821 cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga
    26881 ctgcccggcg cgatagagat cgctgcctaa gcccgcccca gcacaacctg cgtctatcca
    26941 ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt ccgatggcaa
    27001 taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa atattttcag
    27061 cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca
    27121 gacggtcatg ccgtagccca ccgcacggcg gatcacttca ctatggatat tgggcgtaac
    27181 gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc
    27241 tgcgccaatc aacgccttgt cgccatacgc atcaacgatg gcgggaatgc tttgctccca
    27301 ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac
    27361 atgcgccagc gcctcgtcgg gcttaatgcc gcgcaaaatg gcgatcagcg gaagtttagt
    27421 ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg
    27481 ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc
    27541 cggcgacaag ggtgatggcg tgttgatggg tcacatagtc gcgcatactg gcaacctctg
    27601 cgccaatcaa caaaccagag agaaattcgc tgacctgttc gcggggaagt gttcccagca
    27661 catgcgaggc gcgaacttca aaaagctgcg gcaatatggc gggcgcatta aggccgcgct
    27721 caaggccagc cgcgaaggca tcggcagagt tttcctgcgg cggcaaacct gcgccaatca
    27781 atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga aaatcgttga
    27841 tttgctggct atcggcctgc acccatttgc aatgcgtacc gggcataaca taaagagagg
    27901 aaggagccag agctcgcgcg ccgattaatt gtgtttcttc gccgcgcatc acattgtggt
    27961 tatcatcatg agagacgcat aatccgggaa taatccagat attgtcgcca acagacgtta
    28021 attgttcgcc aatagacgaa aaacgggcag gaacggataa atacggtgca actttccagc
    28081 cgacattgct gccgaccatt cctgccatca ccactggcgt attctcttca cgccagtcgg
    28141 tcgtgacttc tgctaacacc gcagccggag attttccgtt caggcgcgtg acgcctgctt
    28201 ctgattgcct gctctccagg cagtggtcgc cctgataaag ccaggcgcgc agattggtcg
    28261 atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga
    28321 gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat gccgtatcgc
    28381 atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat cgcccatcca
    28441 ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt gctgtaacac
    28501 cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt cgttaaacgc
    28561 ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct gcgccagatc
    28621 gctggaagtc gcgcgctctg ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac
    28681 ttcgctcatg gcactgataa gccgtgggtc gtagtcgttt tccaacaccc attgcagtac
    28741 gtcagtgtcg aggtaattcc actggttacg cggtgccaca aacgccccgc gataacgttt
    28801 catttcaatc agccgcttcg ccatcagcga acggaacacc tcacggatga tgttgcgcga
    28861 ggttgcaaac tcttcacaga gctccgcctc agccggaagt ggcgagccag gtacgtattt
    28921 gccgtgaacg atctgtttac ccagcgtaat gacaatgcga tcggttttat tgagagtcat
    28981 ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg cataacgcgg
    29041 caactttgta gtaccagcgt gatgacgttc gcgtttgccg tgtgcgtaat gtagtacaaa
    29101 cttatattgt tgtactacaa tttagatcac aaaaagaaca atgcataaaa aaatgacatg
    29161 cgtcgggcag aaatctgaaa agggatatca ggcgctaaac aggagggaaa gaagagtatg
    29221 ctttcaacgg cttagctact cgtttaaagg attaatcatg aagttgaatt ttaagggatt
    29281 ttttaaggct gccggtttat tcccactggc gctgatgctt tcaggctgta tctcgtatgc
    29341 tctggtttcc cataccgcaa agggtagttc aggaaagtat caatcgcagt cagacaccat
    29401 cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa ggctatgttt ttgtagggga
    29461 atcgctggat taccttatca ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc
    29521 agcacttaac cggcacaata ttcaggttgc cgatgacgca agatttgttt taaatgcggg
    29581 gaaaaagaaa tttaccggca caatatcgct ttactactac tggaataacg aagaagaaaa
    29641 ggcactggca acgcattatg gttttgcctg tggtgttcaa cactgtacca ggtcactgga
    29701 aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg gattactcaa aggtgatggc
    29761 gttctaccat ccatttaaag tgcgatttta tgaatactat tcacccagtg gcattccgga
    29821 tggtgtttcc gcagcattac tgccagtgac tgttacgctg gacatcatta ctgcaccgct
    29881 gcaatttctg gttgtatatg cagtaaacca ataatcagta agcgggcaaa cgcattcatg
    29941 ctgtttgccc gcccacagat taattcagca catacttctc aatagcaaac gccacgccat
    30001 cttcaaggtt agatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    30061 ccatcgccac accgacgcct gcatattcaa tcattgcgat atcgttttcc tgatcgccaa
    30121 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acccccgtac
    30181 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtat
    30241 atttctcttt cacttcctgc ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat
    30301 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    30361 ccagcggaat ggtggcaacg aaggattcat gcaccgtgta gtagctgata tcacggttgg
    30421 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    30481 tttccaggaa acgatagtcg tcatagctga gagcagtttg cgccacggtg ctaccatcag
    30541 cggccttctg taccagcgca ccgttataag taatgcagta gtcgcccggc tgttccatat
    30601 gcagctcttt caggtagttg tgcacacctg catacgggcg acccgtcgtt agcacgacat
    30661 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    30721 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    30781 caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    30841 cgagggaaaa gggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt
    30901 tccctttcag cagttccata cctgcggaaa gcagatcgtt attggcttgt ggtgacactt
    30961 caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt aaactggaag
    31021 ctgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    31081 ccgattccag ttgctcgcca ctaaccgatt gattgccctg ttgattactc agccaggttg
    31141 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccgccct
    31201 gctcctcaac ccaacttaaa atagcctgat atttccccgc atcgcctttc agaaaggcac
    31261 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
    31321 aaagtataga tttgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
    31381 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat ccggcctaca
    31441 aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg tagcgcatca
    31501 ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc
    31561 ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaactggtc
    31621 agcggcaatc gcatccttaa cggtaacgcg cagcatacga cgactttccg ggtccatggt
    31681 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    31741 accgcgacga gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    31801 acgacgctcg ccacgttcga taaatgcatc ttcttccagc aagccacgca gtttctcacc
    31861 cagcgtgcag atacgacgat attcgccacc agtgataaat tcgtgatcca gcggatagtc
    31921 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    31981 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    32041 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
    32101 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    32161 cattttctgc gtcgcgttgt actcagacac caatttctct aacgcttcgc cagccaatgc
    32221 cggtgcgctg gcgttggtgt gcagcgttgc gccatccagc gcgatagaga tctggtactg
    32281 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    32341 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    32401 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    32461 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
    32521 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    32581 aacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    32641 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    32701 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    32761 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    32821 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
    32881 catctgctgt tcaaccgctg atttcacctc agaagaaacc agtttgtctt tggtctggga
    32941 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    33001 gtcaccggtg gcgctaactt tggctttttt gctgtagcct tctttgtcca tgtaggcgtt
    33061 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    33121 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    33181 ttcgacgcca ataccgtctt tttcagtgga gaagtagaag atattcgggt ggatcggcgt
    33241 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
    33301 ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    33361 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    33421 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccaa
    33481 cggtgcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    33541 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    33601 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    33661 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    33721 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    33781 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    33841 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    33901 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    33961 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    34021 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    34081 ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    34141 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    34201 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    34261 cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat
    34321 cgcgttcaaa attacgttcc agcacctcag catattctgt ctctttctcc cagccgcgct
    34381 ggaaagagaa agtcagagag aactcaggga gaaattgctt acaagtatcg gccatatcag
    34441 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca
    34501 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
    34561 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    34621 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    34681 cttcaggcgt tatcagctgc atcggcatca ggtgcgccaa ctccgcaacc ttatgcccgt
    34741 cagtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    34801 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc tcatggcgaa
    34861 tgacgcgacc aatctgcaaa ctacgaaacg cccgaccatg gccgagcgta tagatggctt
    34921 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    34981 aggcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
    35041 ttacagtctc attggcatga caacataagc cgcgctctgg ctggccgcat cttcaatctg
    35101 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
    35161 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    35221 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcggtgattt tcagctggtt
    35281 ttcgctgaca taaagacgta cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
    35341 aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccgggt tcttcggtag
    35401 aacgcggcgg taatccggga agcgaccatc caccagtttg gaggtgaaga taaagtcgcc
    35461 aacgtgggca cgaatgttat tgctgccaat ctgcacgtgc agcggattgt cgccgccgtc
    35521 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
    35581 agattgacca attggcattg aacagactgc cagacggtga ccatcggtcg ctacggtgcg
    35641 cagttcttcg ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg
    35701 cgccatcgaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    35761 ttcgacttca ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
    35821 cgaaaaacgg ctacgcccgg agcgtaccag catccgttca ccttccagct gcacggcaat
    35881 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    35941 cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca tctcgagatc
    36001 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    36061 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    36121 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
    36181 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
    36241 ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg tggttagtca
    36301 gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    36361 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    36421 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    36481 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcccctt
    36541 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcgcccggca
    36601 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
    36661 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    36721 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    36781 gggcgttgaa ggtatggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    36841 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    36901 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    36961 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    37021 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
    37081 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    37141 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
    37201 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    37261 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    37321 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    37381 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    37441 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    37501 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    37561 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    37621 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    37681 tcctgcaaaa cgatcgggac cgtggatcat agcctaaact gcgcaagaga tcttctgttt
    37741 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    37801 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    37861 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    37921 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc
    37981 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    38041 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    38101 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    38161 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    38221 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    38281 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    38341 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    38401 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    38461 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    38521 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    38581 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    38641 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    38701 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    38761 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    38821 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    38881 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    38941 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    39001 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    39061 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    39121 ggtggtgatg ttgagcaagc tctgctgccg gcttacccga aagagctgaa ctctacccag
    39181 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    39241 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    39301 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    39361 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    39421 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac ctttggtcag
    39481 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    39541 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc
    39601 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    39661 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    39721 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    39781 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    39841 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    39901 atctctcagc cgctgttcaa actgctgaaa tggatccaca gctttgtggg taactggggc
    39961 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    40021 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    40081 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
    40141 aaggttaacc cactgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    40201 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    40261 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    40321 ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca gaagatcatg
    40381 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    40441 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    40501 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    40561 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    40621 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    40681 acgcctccgg gacgtggcgg tgttggcatc ctgcgcatct ccggcctcaa agcccgtgaa
    40741 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    40801 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    40861 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    40921 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    40981 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgccgat
    41041 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    41101 ttctccgcac gggtcaacca tctggtggaa gccctcaccc acttgcgcat ttacgtcgaa
    41161 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    41221 cagcttaatg acgtgattgc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    41281 ttgttgcgcg aagggatgaa ggtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    41341 ctgttaaacg cgctggcggg ccgtgaagca gcaatcgtaa ctgatatcgc cggaaccaca
    41401 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgatacc
    41461 gctgggctac gtgaagccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    41521 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac agacgccgtg
    41581 gatccggcag agatctggcc ggaatttatt gcccgtctgc cagcgaaact gccgatcacc
    41641 gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    41701 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    41761 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgc
    41821 cacctacagg cgctggaaca ggcagcggaa catctacaac agggcaaagc gcaactgttg
    41881 ggagcctggg caggtgaact gctggcggaa gagttgcgtc tggcacagca gaacttaagc
    41941 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    42001 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    42061 ttgttagcac tcctgttatt cctcaaccct ttttttaaac attaaaattc ttacgtaatt
    42121 tataatctta aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    42181 ttaaacaatt tt
//
LOCUS       DAZFAH010000019        41870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFAH010000019 DAZFAH010000000
VERSION     DAZFAH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.019"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            175..1392
                     /gene="alaA"
                     /locus_tag="ACHJWB_000964"
     CDS             175..1392
                     /gene="alaA"
                     /locus_tag="ACHJWB_000964"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4312640.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHJWB_000965"
     CDS             1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHJWB_000965"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4312641.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFQLSLDEISQDSPL"
     gene            complement(2134..3966)
                     /locus_tag="ACHJWB_000966"
     CDS             complement(2134..3966)
                     /locus_tag="ACHJWB_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4312642.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4053..4703)
                     /gene="hxpA"
                     /locus_tag="ACHJWB_000967"
     CDS             complement(4053..4703)
                     /gene="hxpA"
                     /locus_tag="ACHJWB_000967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4312643.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGNVIIQ"
     gene            complement(4714..5208)
                     /locus_tag="ACHJWB_000968"
     CDS             complement(4714..5208)
                     /locus_tag="ACHJWB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4312644.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5291..5746)
                     /gene="yfbV"
                     /locus_tag="ACHJWB_000969"
     CDS             complement(5291..5746)
                     /gene="yfbV"
                     /locus_tag="ACHJWB_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4312645.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6084..7286
                     /gene="ackA"
                     /locus_tag="ACHJWB_000970"
     CDS             6084..7286
                     /gene="ackA"
                     /locus_tag="ACHJWB_000970"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4312646.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7361..9505
                     /gene="pta"
                     /locus_tag="ACHJWB_000971"
     CDS             7361..9505
                     /gene="pta"
                     /locus_tag="ACHJWB_000971"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4312647.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9695..11215
                     /gene="yfcC"
                     /locus_tag="ACHJWB_000972"
     CDS             9695..11215
                     /gene="yfcC"
                     /locus_tag="ACHJWB_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4312648.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11248..11790)
                     /gene="yfcD"
                     /locus_tag="ACHJWB_000973"
     CDS             complement(11248..11790)
                     /gene="yfcD"
                     /locus_tag="ACHJWB_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4312649.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11848..12399)
                     /gene="yfcE"
                     /locus_tag="ACHJWB_000974"
     CDS             complement(11848..12399)
                     /gene="yfcE"
                     /locus_tag="ACHJWB_000974"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4312650.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12455..13099)
                     /gene="yfcF"
                     /locus_tag="ACHJWB_000975"
     CDS             complement(12455..13099)
                     /gene="yfcF"
                     /locus_tag="ACHJWB_000975"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4312651.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLAIMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13235..13882
                     /gene="yfcG"
                     /locus_tag="ACHJWB_000976"
     CDS             13235..13882
                     /gene="yfcG"
                     /locus_tag="ACHJWB_000976"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4312652.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13939..14301
                     /gene="folX"
                     /locus_tag="ACHJWB_000977"
     CDS             13939..14301
                     /gene="folX"
                     /locus_tag="ACHJWB_000977"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4312653.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14322..15215
                     /locus_tag="ACHJWB_000978"
     CDS             14322..15215
                     /locus_tag="ACHJWB_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4312654.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15263..16153)
                     /gene="rpnB"
                     /locus_tag="ACHJWB_000979"
                     /pseudo
     CDS             complement(15263..16153)
                     /gene="rpnB"
                     /locus_tag="ACHJWB_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
     gene            complement(16350..17123)
                     /gene="hisP"
                     /locus_tag="ACHJWB_000980"
     CDS             complement(16350..17123)
                     /gene="hisP"
                     /locus_tag="ACHJWB_000980"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4312655.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17131..17847)
                     /locus_tag="ACHJWB_000981"
     CDS             complement(17131..17847)
                     /locus_tag="ACHJWB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4312656.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17844..18530)
                     /gene="hisQ"
                     /locus_tag="ACHJWB_000982"
     CDS             complement(17844..18530)
                     /gene="hisQ"
                     /locus_tag="ACHJWB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4312657.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18620..19402)
                     /gene="hisJ"
                     /locus_tag="ACHJWB_000983"
     CDS             complement(18620..19402)
                     /gene="hisJ"
                     /locus_tag="ACHJWB_000983"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4312658.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19623..20405)
                     /gene="argT"
                     /locus_tag="ACHJWB_000984"
     CDS             complement(19623..20405)
                     /gene="argT"
                     /locus_tag="ACHJWB_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4312659.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20671..21240)
                     /gene="ubiX"
                     /locus_tag="ACHJWB_000985"
     CDS             complement(20671..21240)
                     /gene="ubiX"
                     /locus_tag="ACHJWB_000985"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4312660.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21335..22852)
                     /gene="purF"
                     /locus_tag="ACHJWB_000986"
     CDS             complement(21335..22852)
                     /gene="purF"
                     /locus_tag="ACHJWB_000986"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4312661.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22889..23377)
                     /gene="cvpA"
                     /locus_tag="ACHJWB_000987"
     CDS             complement(22889..23377)
                     /gene="cvpA"
                     /locus_tag="ACHJWB_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4312662.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23636..24298)
                     /gene="dedD"
                     /locus_tag="ACHJWB_000988"
     CDS             complement(23636..24298)
                     /gene="dedD"
                     /locus_tag="ACHJWB_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4312663.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24288..25556)
                     /gene="folC"
                     /locus_tag="ACHJWB_000989"
     CDS             complement(24288..25556)
                     /gene="folC"
                     /locus_tag="ACHJWB_000989"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4312664.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLKSILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTTSFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25626..26540)
                     /gene="accD"
                     /locus_tag="ACHJWB_000990"
     CDS             complement(25626..26540)
                     /gene="accD"
                     /locus_tag="ACHJWB_000990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4312665.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26696..27355)
                     /locus_tag="ACHJWB_000991"
     CDS             complement(26696..27355)
                     /locus_tag="ACHJWB_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4312666.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27438..28250)
                     /gene="truA"
                     /locus_tag="ACHJWB_000992"
     CDS             complement(27438..28250)
                     /gene="truA"
                     /locus_tag="ACHJWB_000992"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4312667.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28250..29263)
                     /locus_tag="ACHJWB_000993"
     CDS             complement(28250..29263)
                     /locus_tag="ACHJWB_000993"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4312668.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29329..30465)
                     /gene="pdxB"
                     /locus_tag="ACHJWB_000994"
     CDS             complement(29329..30465)
                     /gene="pdxB"
                     /locus_tag="ACHJWB_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4312669.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30564..31559
                     /gene="flk"
                     /locus_tag="ACHJWB_000995"
     CDS             30564..31559
                     /gene="flk"
                     /locus_tag="ACHJWB_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4312670.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31556..32734)
                     /locus_tag="ACHJWB_000996"
     CDS             complement(31556..32734)
                     /locus_tag="ACHJWB_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312671.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRV
                     "
     gene            complement(33018..34238)
                     /gene="fabB"
                     /locus_tag="ACHJWB_000997"
     CDS             complement(33018..34238)
                     /gene="fabB"
                     /locus_tag="ACHJWB_000997"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4312672.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34397..36403
                     /gene="mnmC"
                     /locus_tag="ACHJWB_000998"
     CDS             34397..36403
                     /gene="mnmC"
                     /locus_tag="ACHJWB_000998"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4312673.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36524..36802)
                     /locus_tag="ACHJWB_000999"
     CDS             complement(36524..36802)
                     /locus_tag="ACHJWB_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4312674.1"
                     /translation="MIAEFESRILALIDGMVDHASDDVLFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36836..37384)
                     /gene="epmC"
                     /locus_tag="ACHJWB_001000"
     CDS             complement(36836..37384)
                     /gene="epmC"
                     /locus_tag="ACHJWB_001000"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4312675.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37384..38193)
                     /locus_tag="ACHJWB_001001"
     CDS             complement(37384..38193)
                     /locus_tag="ACHJWB_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4312676.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38193..39017)
                     /gene="mepA"
                     /locus_tag="ACHJWB_001002"
     CDS             complement(38193..39017)
                     /gene="mepA"
                     /locus_tag="ACHJWB_001002"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4312677.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPKLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39021..40106)
                     /gene="aroC"
                     /locus_tag="ACHJWB_001003"
     CDS             complement(39021..40106)
                     /gene="aroC"
                     /locus_tag="ACHJWB_001003"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4312678.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40141..41073)
                     /gene="prmB"
                     /locus_tag="ACHJWB_001004"
     CDS             complement(40141..41073)
                     /gene="prmB"
                     /locus_tag="ACHJWB_001004"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4312679.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41239..41790
                     /gene="smrB"
                     /locus_tag="ACHJWB_001005"
     CDS             41239..41790
                     /gene="smrB"
                     /locus_tag="ACHJWB_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4312680.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
BASE COUNT        10291 a        11373 c        10503 g         9703 t
ORIGIN      
        1 gcatttacac ctaattattt tgtcattatt aagttattaa cagcgcaatc gagccttccc
       61 ctctggcaaa atcttattct gcagaccttc aaaacaccct cctgggggag tacattgttc
      121 taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc
      181 cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg
      241 aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac
      301 ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg
      361 cctacagctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg
      421 cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat
      481 ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg
      541 ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa
      601 gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc
      661 gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc
      721 gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc
      781 attatcttcg ccgatgaaat ttatgacaaa attctctacg acgatgctga gcatcactca
      841 attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac
      901 cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa
      961 ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg
     1021 caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct
     1081 ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc
     1141 gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc
     1201 tttaacattc atgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg
     1261 ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta
     1321 ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat
     1381 catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagtgtt atcccgccac
     1441 aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc
     1501 atctctcccg cctgaaactc attaaccgct ggccgctaat gcgcaacgtg cggacggaaa
     1561 atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa
     1621 atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg
     1681 atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa
     1741 tcgctcagga gtacaaggct attgaaaaaa tcgctcagca gaaactggtc gatatggttc
     1801 cggaagaact gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag
     1861 aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac
     1921 tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag
     1981 cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc ttccaacttt
     2041 cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag
     2101 gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat
     2161 caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact
     2221 gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat
     2281 aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat
     2341 cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat
     2401 aaatagccca ataaccgccg acaagacaaa caaacacccc agcatcatat gtggcccgta
     2461 accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa
     2521 tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt
     2581 ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc
     2641 ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa
     2701 aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt
     2761 cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac
     2821 aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac
     2881 gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg
     2941 caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc
     3001 aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact
     3061 acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc
     3121 gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc
     3181 aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc
     3241 aatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc
     3301 acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc
     3361 ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag
     3421 gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact
     3481 gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctta tcagcccggc
     3541 aaaactcaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa
     3601 caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc
     3661 gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag
     3721 ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa
     3781 ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg ggactgtcag
     3841 cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct
     3901 gcccgtcgca aacaagacaa tggcaacggc cagtaatgaa agaacccaaa tcaattcacc
     3961 gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc
     4021 gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatt gccatttggc
     4081 tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc
     4141 ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc
     4201 acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc
     4261 gcacctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca
     4321 aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag
     4381 ccagaagtca caatggccca cggaatacct gctttattca aatgattgag taaggcgatt
     4441 gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatgtgctc cagacgcgta
     4501 aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcaa agaggtgatc
     4561 gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc
     4621 cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca
     4681 aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc
     4741 gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata
     4801 tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata
     4861 gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc
     4921 gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga
     4981 ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc
     5041 ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc
     5101 aataattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat
     5161 cttgtactga ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg
     5221 tatcactata ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat
     5281 caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa
     5341 gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag
     5401 actcctgcaa tttaccgcga acttcataaa accagttaag gattgcaggg ggtaatggcg
     5461 ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg
     5521 cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata
     5581 cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga
     5641 caaagaccgg ggcaaggcgt ttttccagtg gccacgtctt tgagtaatgc tgtccgcggc
     5701 gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact
     5761 tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa
     5821 cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta
     5881 catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa
     5941 tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta
     6001 atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc
     6061 acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta
     6121 gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt
     6181 tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac
     6241 aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata
     6301 ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg
     6361 tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta
     6421 tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag
     6481 aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt
     6541 tccaccagac tatgccggaa gagtcttacc tctacgccct gccttacaac ctgtacaaag
     6601 agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag
     6661 cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca
     6721 acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga
     6781 ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct
     6841 tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag
     6901 agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact
     6961 acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat
     7021 acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcactggtg
     7081 gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg
     7141 gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca
     7201 acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc
     7261 aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt
     7321 tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct
     7381 gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat
     7441 ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg
     7501 cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc
     7561 tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct
     7621 gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt
     7681 tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc
     7741 taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca
     7801 gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat
     7861 caccggcgtt atcgttaaca aactgaacgc accggttgat gaacagggtc gtactcgccc
     7921 ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc
     7981 gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct
     8041 gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg
     8101 cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat
     8161 gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct
     8221 ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg
     8281 cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct
     8341 gccggtattt atggtgaaca ccaacacctg gcagacctct ctgagcctgc agagcttcaa
     8401 cctggaagtt ccggttgacg atcacgaacg tatcgagaaa gttcaggaat acgttgctaa
     8461 ctacatcaac gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct
     8521 gtctccgcct gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat
     8581 cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg
     8641 tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc
     8701 tcagggtgta gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag
     8761 ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg
     8821 cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga
     8881 tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct
     8941 gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga
     9001 acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc
     9061 agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc
     9121 tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga
     9181 agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta
     9241 cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg
     9301 tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt
     9361 acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt
     9421 taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc
     9481 gattcagtct gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat
     9541 ctgaaccgga aataatcact atttccggtt ttttattctc ttaatctgca ttaatccttt
     9601 ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatctcact ttgcatactt
     9661 accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg aatctaaacc
     9721 aaccagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac
     9781 cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca
     9841 ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac
     9901 taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg
     9961 cccgggcctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat acgggacagc
    10021 tgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac
    10081 aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct
    10141 ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga
    10201 agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag
    10261 tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat
    10321 gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc
    10381 cgggttgcgc atcgtggtgt gggttatcgc cactatgatt ggcctgatct ttaccatggt
    10441 gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt
    10501 ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt acctttggtg actggctggt
    10561 attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg
    10621 gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg
    10681 cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg
    10741 ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct
    10801 ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat accatcctca acagcattgc
    10861 caatgccatt agcggtctgg acaacgcggt cgcggcctgg tttatgttgc tcttccaggc
    10921 ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca tgccgttact
    10981 ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cattccagtt
    11041 tggtgatggc ttcagtcaca tcatttaccc aacctcagct tcgttaatgg caactctcgg
    11101 tgtgtgcagg gtggacttcc gtaactggct gaaggtgggc gcgaccctac ttggactgct
    11161 gtttattatg tccagcgtcg tagtgatcgg cgctcagttg atgggctacc actaaaaatg
    11221 ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt
    11281 tttggcattg cgcttcatcc acaacgccag cgctttcagc gagtctggag tgaactcatc
    11341 gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg
    11401 tagggcgaag ggaccgtgag agacgcagct gaacaacgcg ccccagacac ggcaattttt
    11461 atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc
    11521 ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctgaacca caccgcctgc
    11581 ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat
    11641 tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg
    11701 catttgttcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca
    11761 ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct
    11821 tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact
    11881 ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg
    11941 attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg
    12001 ttctgccact ggcagatggg tatgaccgta caccagcaca tcgttctggt ttaaagcagg
    12061 tagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa
    12121 tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc
    12181 gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt
    12241 ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag
    12301 gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg
    12361 taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa
    12421 acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg
    12481 caataaaacg ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcgtt
    12541 ctggcacctc atcgccatgt agtaccaggc ggttaatcat tatcgccaga tcagtatcag
    12601 caatgcacca ttcaccaaat aaattcggct gacccagtgc taacaaatgt tctgccatcg
    12661 cgaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg
    12721 caaagacaac atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcagccagg
    12781 cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg
    12841 tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa
    12901 aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct
    12961 gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct
    13021 gcaacgccac ccaggcggat aacacataag gggagaaaaa gtgggcatct gaccaaagcg
    13081 tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag
    13141 agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt
    13201 gtggtgttca gatgattaaa caaccggagc tgcaatgatc gatctctatt tcgccccgac
    13261 acccaatggt cacaaaatta cgctgtttct cgaagaagca gagcttgatt atcgcttgat
    13321 taaggtagac ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca tttcgcccaa
    13381 caacaaaatt ccggcaattg ttgatcattc tcctgccgat ggcggcgaac cgttaagcct
    13441 ctttgagtct ggtgccattt tgttgtatct ggctgagaaa acaggactct ttttgagtca
    13501 tgaaacgcgt gagcgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg
    13561 gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc
    13621 tattgaacgt tatcaggttg aaactcagcg tctttaccat gtactgaaca agcggctgga
    13681 aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg
    13741 ggttaatgcc tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg
    13801 gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg
    13861 tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa
    13921 aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt
    13981 ttgcgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc
    14041 aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg
    14101 ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct
    14161 ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg
    14221 tatgctgaag tggagatcga taaactacac gcgctgcgct acgccgattc ggtatccatg
    14281 accttgagct ggcagcgtta accgccatat cgggaggctg catgaatata gtgatcaccg
    14341 gagggacagg attaattggt cgccatttaa tcccgcgttt gctggagttg ggccatcaaa
    14401 tcacggtagt gacgcgtaac ccgcagaaag ccagttccgt tctcggccct cgggtgacac
    14461 tatggcaagg gcttgccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg
    14521 ccggagaacc gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa
    14581 gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccaccgt
    14641 cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta
    14701 ccgaagagga accgccgcat aacgaattta cccataaact ctgcgcccgc tgggaagaaa
    14761 ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgtacc ggtgtagtgc
    14821 tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg
    14881 ggccgattgg ctctggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg
    14941 gtattctctg gctgctggat aacgacctgc gcgggccatt taatatggtt tcgccctacc
    15001 cggtacgcaa tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc ccggccattt
    15061 tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcaata ctggtattag
    15121 gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg
    15181 atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg cttacagcaa acatccgcca
    15241 gttaactccc ggtgttacag gattagtggc tttgcgcgat aagatcgtct ggtgaaagtc
    15301 gggtcaccat cataactaac tctctgtcta aacctctatc cagcatctcc tgagcaatac
    15361 gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg
    15421 tcatcagttt ctccttttct tgtggtgcgc gttccgctat ctcaccaata aatgcacgaa
    15481 aacgctgggc atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat
    15541 tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct
    15601 gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt
    15661 catccggcac cacggtaata tccaccaacg gaaacgctga tgaatatatt ttgcgggcta
    15721 tagcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac
    15781 catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt
    15841 tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac
    15901 tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca
    15961 agaggtcgga ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa
    16021 gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc
    16081 gcgcggtgtc tggatggcgt caaaaagatt taaataccgc atcatgcggc gtggaagttg
    16141 tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg
    16201 attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt
    16261 agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc atgaaaatac
    16321 tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc
    16381 agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttc
    16441 ccctgatgga ggaaaatgac atgagtagaa acatggcgag caaagcccat ttcgtgagtc
    16501 actaccacca tggttttccc ctcttctgcc agttgctgca taatacgcaa cacttcgcct
    16561 accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc
    16621 gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat
    16681 ttcccctgcg cacgttcgtc tatcccgact tttgccagat acttcaccgc ccgctcgcgc
    16741 gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc
    16801 gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag
    16861 cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc
    16921 gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc
    16981 agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc
    17041 gcttgcagtg agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata
    17101 acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg
    17161 caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata
    17221 gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg
    17281 ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat
    17341 caggatcact tcgttgctgt atgccggtaa cgcaatacgt agcgcagaag gcaaaataat
    17401 gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg
    17461 cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt
    17521 cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc
    17581 cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg
    17641 cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga
    17701 gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag
    17761 cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt
    17821 ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg
    17881 cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca
    17941 cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg
    18001 accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag
    18061 ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac
    18121 acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc
    18181 ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata
    18241 ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg
    18301 tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct
    18361 cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta
    18421 ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc
    18481 agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc
    18541 ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc
    18601 tttctttcac cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta
    18661 atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca
    18721 gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt
    18781 taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc
    18841 tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa
    18901 tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac
    18961 cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga
    19021 ctgtcggctg aatgtcagaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt
    19081 tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg
    19141 cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg
    19201 tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca
    19261 gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga
    19321 tgttttgcgg aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga
    19381 gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata gggaatattt
    19441 acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc
    19501 gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag
    19561 tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg
    19621 tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg
    19681 tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta
    19741 cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca
    19801 aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct
    19861 tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca
    19921 taggccacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt
    19981 gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt
    20041 gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc
    20101 tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct
    20161 ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc
    20221 cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct
    20281 ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc
    20341 gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc
    20401 ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt
    20461 gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa
    20521 atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg
    20581 aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca
    20641 aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc
    20701 ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc
    20761 atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat
    20821 ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca
    20881 gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc
    20941 agtatagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc
    21001 cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac
    21061 atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg
    21121 cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa
    21181 taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat
    21241 aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag
    21301 tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat
    21361 tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga
    21421 aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt
    21481 caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct
    21541 ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag
    21601 cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg
    21661 gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct
    21721 gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga
    21781 actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg
    21841 gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc
    21901 ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca
    21961 cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat
    22021 tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata
    22081 catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact
    22141 gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc
    22201 ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa
    22261 tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac
    22321 cgtggccgat aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag
    22381 caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc
    22441 tagcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt
    22501 cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg
    22561 taatgccata cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag
    22621 ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag
    22681 cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat
    22741 ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat
    22801 aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt
    22861 ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt
    22921 gcagataatc aaaaaaccat ctgatgataa aactgaattg cgggatcagc tgtgatttgc
    22981 tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcggcaa
    23041 caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca
    23101 accccgtttt ctccaccaac tggcctatca cgaagttcac gatagcacca acgatcaggg
    23161 tagcgataaa cagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg
    23221 taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg
    23281 tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag
    23341 caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc
    23401 tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct
    23461 tcccacgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc
    23521 tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt
    23581 ctaacactgc ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga ctgtattaat
    23641 tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg
    23701 aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct
    23761 gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa
    23821 tctcattcac tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg
    23881 tcggtgcagc tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt
    23941 caaccttcgg tttaggcggc tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt
    24001 ccggttcaaa ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac ggtgctgcgg
    24061 catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg
    24121 tggcggctgg catcatatca ggctcatcgc gatcgcccgc ttttggaacc agcgggatag
    24181 ccgcaaactc atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca
    24241 cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg
    24301 cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg tgtggaaaga accacacacc
    24361 agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta
    24421 tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc
    24481 ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg
    24541 gcaatatctt tatcatgtag cataccgata accgccaaca cgcgcccgtt tttcggtagc
    24601 gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata
    24661 acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg
    24721 tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgctgtt
    24781 gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg
    24841 ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtagc
    24901 agtgcacctt tttcctgcgc cacatcagca atggtagaag gcatttccgg ctcaccgaca
    24961 attgctggtt tttcgctgcg gaagatgcct gctttctcgc gaccaatact ttcacgatct
    25021 ggccccagcc agtcggtatg atccagcgca atactggtta ctaccgcgac atcggcatcg
    25081 acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt
    25141 tgtgcctgtt tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa
    25201 atatcaccgc gtgccgattc aatctccgca aaagaggtgg tgtgggccga ttccggcaat
    25261 tcctgaccct gcacacgtac gcgctcggta taacgcacca gatgaggcga actgtagacg
    25321 cccactttgt accctgccgc catcagaatc gacttcagcg tacggcaggt ggtgcctttg
    25381 ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcaggacgcc aagacgcgcc
    25441 gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga
    25501 taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta
    25561 tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc
    25621 agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc
    25681 gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg
    25741 cgcatttccg gacgacggac gatcatgtcg atggcgcctt tctcgatcag gaattcactg
    25801 cgctggaatc caggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca
    25861 aagccgatta acgctttagg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca
    25921 gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc
    25981 atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc
    26041 atacgtgcgc caccagaggc ggagaagcag atcagcgggc agttatcttc cagcgcctgc
    26101 tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg
    26161 aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc
    26221 gcatcttttt cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag
    26281 tcacgaaact tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct
    26341 tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc
    26401 ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca
    26461 cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta
    26521 attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt
    26581 atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa
    26641 aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt
    26701 ttgcggcgcg tgccgcagcg cgtttgtgac ggattatttc gatgacaccc ggcaatatag
    26761 aaaccacaat aatcccgacg atcagcagct taaggttatc ctgaaccatc gggattgtac
    26821 cgaagaaata gcctgcgtag gtaaaaagca gtacccacaa taatgcgccg atcacgttat
    26881 aagcggcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggg gcgaacgttc
    26941 tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc gccatgtttc tcataaaact
    27001 gatgggtttt gtcgagataa ctgcgacgga aaattttcga attgggatta ctgaacaact
    27061 tttcaccgaa caaccgcccg atagtgtaat tgaccgcgtc acccacaatc gcggcgatca
    27121 gcatcagcac caccatcata tggacgttga gatcgttggt ttccagtgat gccaacgctc
    27181 cggcaacaaa cagcaatgaa tcacccggta aaaacggcgt taccaccaga ccggtttcac
    27241 agaacaaaat taagaacaaa atggcataaa cccagacgcc gtactccgcg accagctctg
    27301 ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg
    27361 cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt
    27421 tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga
    27481 tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct
    27541 gccgccagcg ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg
    27601 tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaagca
    27661 ttcgctttaa tatctaccac cacataagga ccgtgacgcg tgacattaat atgcataacg
    27721 ttgcgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg
    27781 cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg
    27841 gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa
    27901 cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc
    27961 acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc
    28021 tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt gtacccctgc gtcagtacgc
    28081 ccggcgcaga agacggtgat gggttcgttc gccacctggg agagcgcctt ttccagcttc
    28141 tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac
    28201 tcaatgccca gcgcaatttt ataaactggc agttgttgct ggtcggacat tagtacagat
    28261 actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt
    28321 tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc
    28381 caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt
    28441 cagagagcac aatatcttcg ccctgagcaa acgcatcacg cgcttcttct gccgccagcg
    28501 gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga
    28561 cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac
    28621 gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca
    28681 gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct
    28741 gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa
    28801 cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc
    28861 tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg
    28921 ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt
    28981 cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc
    29041 ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg
    29101 tgattgtttt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat
    29161 aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag
    29221 cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa
    29281 atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg
    29341 atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca
    29401 aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa
    29461 ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac
    29521 caaatgcacc agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc
    29581 aaactctggc gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt
    29641 gctataggct tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tatagcctgc
    29701 gatatgcggc gtgccgatat ccacttttgt cagcaattcc acgttaagtt ccggttcgcc
    29761 ttcccagaca tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc
    29821 agtattatcg acgactgcgc cacggcatgc gttaatcaga atcgctcccg gcttcaggct
    29881 acgaatcagt ttttcatccg ccagatgcag cgttttgtac ggcccgtctt tgaagagtgg
    29941 cgtatggaaa gtcagaatat ccgcatgctg aactaactca tccagcgagc ggaaatcacc
    30001 ctcatcccca cggtcagcgc gaggcggatc gcaaagcaag gttttaatcc ctaacgcttc
    30061 cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc
    30121 gtgcagtgaa aatccatcgc gttcggcaag catcagtagt gaggagaaaa catattccac
    30181 caccgcaatc gcattacagc caggtgccgc ggaaaaacca attcccgcct gctttaacca
    30241 tgcttcatcg acatggtcgg tccccgctgt ggcagtacca acaaatttaa tgggttttcc
    30301 tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc
    30361 cagttgagcg acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa
    30421 taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt
    30481 atgagacgag agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa
    30541 gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcagc
    30601 caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc
    30661 aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa cagcaaagag
    30721 cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt
    30781 cgcgtcactt ccctgccgct gaacacaacc tggcgcaacg tctgctggcc gcgcaaaaaa
    30841 gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc
    30901 gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg aatcagctct
    30961 caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc
    31021 aaccgcagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctta
    31081 aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat
    31141 cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc
    31201 atctggtgac ctggctgcag gcgcgtcaga cgctaagcca gcaaaatacg ccgacgctgg
    31261 aatcactaca gatggcgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg
    31321 catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg
    31381 aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc
    31441 aaccgctgct taatcctttt ccaccaatga tggacacgtt gcaaaatatg gcaacgcgtc
    31501 ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttaaa
    31561 cccgacgaaa tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa
    31621 gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc
    31681 cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg
    31741 ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg
    31801 ttaacgccgc gcccgctaat gcgacccacg cacctggggc ttgccagagc aacaacaagc
    31861 ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca
    31921 tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc
    31981 ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa
    32041 aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc
    32101 acagcgatgg acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc
    32161 acgccagtaa gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat
    32221 gaatcaacag gccaagcggc gcaccaacag cgagggcacc gtaaatcgcc attccattcc
    32281 atgacatcac tttgccagag tgttttgccc ctacgatgcc taacccccag gtcagagcgc
    32341 ctgtcagtaa ctggctttca ccaaagccaa gaatcaaacg cccgatgacc aacagggcaa
    32401 atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac
    32461 cacaagctaa catcccctga agcgccgaac gttttgcgcc atattgatcg gccagtcgcc
    32521 cggcatagcc acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca
    32581 tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc
    32641 ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag
    32701 aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg
    32761 cgaggagtat ccccgtctca tctctctggt ttcagggtta cagtgcgttg gcaggattta
    32821 acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aactggcggc
    32881 gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc
    32941 cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta
    33001 ctccgaccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    33061 ccgaagccga agctgttaga cataacggtg gtcagttcgc gatcggtcgt ttcggtcacg
    33121 atgttcagac ctgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    33181 ccgtgttcca gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag
    33241 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    33301 atagctgcca gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtaa
    33361 tcgattgggg tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc gccttcgcca
    33421 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    33481 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    33541 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggtgtg
    33601 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    33661 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    33721 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    33781 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    33841 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaatacc
    33901 tggaaacgtg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    33961 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    34021 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    34081 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    34141 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    34201 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    34261 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    34321 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    34381 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    34441 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    34501 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    34561 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    34621 gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    34681 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    34741 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    34801 gcccggttgc catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg
    34861 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    34921 gtttctggac ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa acctgtttaa
    34981 cgccatggca aagttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt
    35041 tgtccgccgc ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg
    35101 caaacgggaa atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc
    35161 gtggtttaac cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc
    35221 cagcgcgttg ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc
    35281 ggatgaggcc cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    35341 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    35401 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    35461 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    35521 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    35581 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    35641 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    35701 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    35761 cgtagtggta ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactctccc
    35821 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    35881 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    35941 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    36001 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    36061 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    36121 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    36181 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    36241 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    36301 cgacgaaccg attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg
    36361 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    36421 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    36481 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    36541 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    36601 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    36661 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    36721 tggcaaacaa cacatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc
    36781 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt
    36841 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    36901 gtagtgcttt aatgaagcgt gccggacgct cgggaatgcc atttgtcaga taatccatca
    36961 cctgtgcatg tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat
    37021 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct
    37081 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcacgc ccatccgggc
    37141 aataccagta gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg
    37201 aaatctcatg aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta
    37261 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    37321 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    37381 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt
    37441 ggcactcatc accgccgaga cgataacgat catcgggcgg atcagttttt gtcctttgct
    37501 cagcaccagt cttgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc
    37561 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    37621 tgcgttgagt aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac
    37681 gaaggccagc gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac
    37741 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc
    37801 acccagcttt ggcatcagca agaaatagag gccgatacaa attaccagaa tgggcaaaat
    37861 ctgccgtaag acatcagcct gaacgtattg caccagcagt gcgccgctca ttgagccgac
    37921 aaaggtcatg gcgatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    37981 gtagatagta gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcatt
    38041 agcgggagac atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    38101 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    38161 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    38221 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    38281 ggaggtgcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt
    38341 tgatcttcac actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga
    38401 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    38461 agttgttgtt taatcgccgg attaacaaaa atgcgtgtga cgtctttgtc ctgggcggcg
    38521 agtttgatca agctgaaaat ttctggcttc cacagcttcg gaacaacgtg tttaccgtcg
    38581 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    38641 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    38701 aaacgcccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    38761 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    38821 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcgccg
    38881 acgatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    38941 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    39001 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    39061 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc
    39121 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    39181 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    39241 tgagcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    39301 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    39361 aagccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    39421 tcggcatcca ggcggtcaaa taccggctcg ccaagtccgg cggggacgcc actggcaaca
    39481 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    39541 aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    39601 tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca
    39661 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    39721 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    39781 taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg
    39841 tcggtatttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    39901 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    39961 cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    40021 ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag
    40081 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    40141 ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat
    40201 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    40261 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag
    40321 gtaatctgcc gcgtttccaa gaatgcgacg cgtcagttta aggccgtcgg tgccagatgc
    40381 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    40441 gacatacggc gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag
    40501 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    40561 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    40621 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    40681 cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac
    40741 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    40801 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    40861 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    40921 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    40981 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    41041 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    41101 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    41161 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    41221 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    41281 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgatcgt ccaccgaccg
    41341 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    41401 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    41461 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    41521 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    41581 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    41641 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    41701 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    41761 gaagagtggt tgccgccgga gttgccctga atgctggcag caaatgccgg agcagcgtaa
    41821 acgccctatc cggcctacaa atgtgtgata attcaataaa ttgtaattca
//
LOCUS       DAZFAH010000020        41594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFAH010000020 DAZFAH010000000
VERSION     DAZFAH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.020"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            289..375
                     /gene="leuL"
                     /locus_tag="ACHJWB_001006"
     CDS             289..375
                     /gene="leuL"
                     /locus_tag="ACHJWB_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4312681.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            468..2039
                     /gene="leuA"
                     /locus_tag="ACHJWB_001007"
     CDS             468..2039
                     /gene="leuA"
                     /locus_tag="ACHJWB_001007"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4312682.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEIKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2039..3130
                     /gene="leuB"
                     /locus_tag="ACHJWB_001008"
     CDS             2039..3130
                     /gene="leuB"
                     /locus_tag="ACHJWB_001008"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4312683.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3133..4533
                     /gene="leuC"
                     /locus_tag="ACHJWB_001009"
     CDS             3133..4533
                     /gene="leuC"
                     /locus_tag="ACHJWB_001009"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4312684.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEDATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4544..5149
                     /gene="leuD"
                     /locus_tag="ACHJWB_001010"
     CDS             4544..5149
                     /gene="leuD"
                     /locus_tag="ACHJWB_001010"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4312685.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5198..6376)
                     /gene="setA"
                     /locus_tag="ACHJWB_001011"
     CDS             complement(5198..6376)
                     /gene="setA"
                     /locus_tag="ACHJWB_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO4312686.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6478..6609)
                     /gene="sgrT"
                     /locus_tag="ACHJWB_001012"
     CDS             complement(6478..6609)
                     /gene="sgrT"
                     /locus_tag="ACHJWB_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4312687.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6698..8353
                     /gene="sgrR"
                     /locus_tag="ACHJWB_001013"
     CDS             6698..8353
                     /gene="sgrR"
                     /locus_tag="ACHJWB_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4312688.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEIDYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8387..8486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8517..9500
                     /gene="thiB"
                     /locus_tag="ACHJWB_001014"
     CDS             8517..9500
                     /gene="thiB"
                     /locus_tag="ACHJWB_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4312689.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9476..11086
                     /gene="thiP"
                     /locus_tag="ACHJWB_001015"
     CDS             9476..11086
                     /gene="thiP"
                     /locus_tag="ACHJWB_001015"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4312690.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11070..11768
                     /gene="thiQ"
                     /locus_tag="ACHJWB_001016"
     CDS             11070..11768
                     /gene="thiQ"
                     /locus_tag="ACHJWB_001016"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4312691.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11882..12646)
                     /gene="yabI"
                     /locus_tag="ACHJWB_001017"
     CDS             complement(11882..12646)
                     /gene="yabI"
                     /locus_tag="ACHJWB_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4312692.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(12732..13610)
                     /gene="araC"
                     /locus_tag="ACHJWB_001018"
     CDS             complement(12732..13610)
                     /gene="araC"
                     /locus_tag="ACHJWB_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4312693.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13949..15649
                     /gene="araB"
                     /locus_tag="ACHJWB_001019"
     CDS             13949..15649
                     /gene="araB"
                     /locus_tag="ACHJWB_001019"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4312694.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15660..17162
                     /gene="araA"
                     /locus_tag="ACHJWB_001020"
     CDS             15660..17162
                     /gene="araA"
                     /locus_tag="ACHJWB_001020"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4312695.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17331..18026
                     /gene="araD"
                     /locus_tag="ACHJWB_001021"
     CDS             17331..18026
                     /gene="araD"
                     /locus_tag="ACHJWB_001021"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4312696.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWALSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18101..20452
                     /gene="polB"
                     /locus_tag="ACHJWB_001022"
     CDS             18101..20452
                     /gene="polB"
                     /locus_tag="ACHJWB_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4312697.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLHLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20617..23523
                     /gene="rapA"
                     /locus_tag="ACHJWB_001023"
     CDS             20617..23523
                     /gene="rapA"
                     /locus_tag="ACHJWB_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4312698.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRKQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23535..24194
                     /gene="rluA"
                     /locus_tag="ACHJWB_001024"
     CDS             23535..24194
                     /gene="rluA"
                     /locus_tag="ACHJWB_001024"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4312699.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24311..25126)
                     /gene="djlA"
                     /locus_tag="ACHJWB_001025"
     CDS             complement(24311..25126)
                     /gene="djlA"
                     /locus_tag="ACHJWB_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4312700.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25381..27735
                     /gene="lptD"
                     /locus_tag="ACHJWB_001026"
     CDS             25381..27735
                     /gene="lptD"
                     /locus_tag="ACHJWB_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4312701.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWKGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27788..29074
                     /gene="surA"
                     /locus_tag="ACHJWB_001027"
     CDS             27788..29074
                     /gene="surA"
                     /locus_tag="ACHJWB_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4312702.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29074..30063
                     /gene="pdxA"
                     /locus_tag="ACHJWB_001028"
     CDS             29074..30063
                     /gene="pdxA"
                     /locus_tag="ACHJWB_001028"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4312703.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30060..30881
                     /gene="rsmA"
                     /locus_tag="ACHJWB_001029"
     CDS             30060..30881
                     /gene="rsmA"
                     /locus_tag="ACHJWB_001029"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4312704.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30884..31261
                     /gene="apaG"
                     /locus_tag="ACHJWB_001030"
     CDS             30884..31261
                     /gene="apaG"
                     /locus_tag="ACHJWB_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4312705.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31268..32116
                     /gene="apaH"
                     /locus_tag="ACHJWB_001031"
     CDS             31268..32116
                     /gene="apaH"
                     /locus_tag="ACHJWB_001031"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4312706.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32141..32740
                     /locus_tag="ACHJWB_001032"
     CDS             32141..32740
                     /locus_tag="ACHJWB_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO4312707.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32776..33243
                     /locus_tag="ACHJWB_001033"
     CDS             32776..33243
                     /locus_tag="ACHJWB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312708.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33342..33521)
                     /locus_tag="ACHJWB_001034"
     CDS             complement(33342..33521)
                     /locus_tag="ACHJWB_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4312709.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33542..34021)
                     /gene="folA"
                     /locus_tag="ACHJWB_001035"
     CDS             complement(33542..34021)
                     /gene="folA"
                     /locus_tag="ACHJWB_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4312710.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34213..36075)
                     /gene="kefC"
                     /locus_tag="ACHJWB_001036"
     CDS             complement(34213..36075)
                     /gene="kefC"
                     /locus_tag="ACHJWB_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4312711.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPLS"
     gene            complement(36068..36598)
                     /gene="kefF"
                     /locus_tag="ACHJWB_001037"
     CDS             complement(36068..36598)
                     /gene="kefF"
                     /locus_tag="ACHJWB_001037"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4312712.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36706..38037)
                     /gene="yaaU"
                     /locus_tag="ACHJWB_001038"
     CDS             complement(36706..38037)
                     /gene="yaaU"
                     /locus_tag="ACHJWB_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312713.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPLLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38096..38383)
                     /gene="fixX"
                     /locus_tag="ACHJWB_001039"
     CDS             complement(38096..38383)
                     /gene="fixX"
                     /locus_tag="ACHJWB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4312714.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKVCP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYSRGTFGVEFRYG"
     gene            complement(38380..39666)
                     /gene="fixC"
                     /locus_tag="ACHJWB_001040"
     CDS             complement(38380..39666)
                     /gene="fixC"
                     /locus_tag="ACHJWB_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4312715.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39717..40658)
                     /gene="fixB"
                     /locus_tag="ACHJWB_001041"
     CDS             complement(39717..40658)
                     /gene="fixB"
                     /locus_tag="ACHJWB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4312716.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40673..41443)
                     /gene="fixA"
                     /locus_tag="ACHJWB_001042"
     CDS             complement(40673..41443)
                     /gene="fixA"
                     /locus_tag="ACHJWB_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4312717.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10060 a        11404 c        10883 g         9247 t
ORIGIN      
        1 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
       61 tcctcgtgga ttagatgcaa aaatttattc tgaaatatgt caaccgatga aaagcgtcgg
      121 tagttaagca gaaattaata tcgcttactt taaccaccgc agcacaatta gctaatttta
      181 cggatgcaga actcatgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
      241 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
      301 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
      361 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
      421 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
      481 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
      541 tgaaagaaaa actgcaaatt gcgctggcgc ttgagcgtat gggtgttgac gtgatggaag
      601 tcgggttccc cgtctcttcg ccgggtgatt ttgaatcagt gcaaaccatc gctcgccagg
      661 ttaaaaacag ccgcgtatgc gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
      721 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgcc acttcgccaa
      781 tgcacatcgc caccaagctg cgcagcacgc tggacgaggt gatcgaacgc gctatctata
      841 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
      901 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc aatcaatgcc ggtgccacca
      961 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     1021 gcggcctgta tgaacgcgtg cctaacatcg acaaagccat tatttccgta catacccacg
     1081 acgatttggg cctggcggtc ggaaactcac tggcggcggt acatgccggt gcacgccagg
     1141 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttccctg gaagaagtca
     1201 tcatggcgat caaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     1261 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     1321 aagccattgt tggcagcggc gcattcgcac actcctccgg tatccaccag gatggcgtgc
     1381 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     1441 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gacgagatgg
     1501 ggtataaaga aagtgaatat aatttagaca atttgtacga cgctttcctg aagctggcgg
     1561 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     1621 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     1681 ttgccaccgc cgccgtcaaa ctggcctgtg gcgaagaaat caaagcagaa gccgccaacg
     1741 gtaacggtcc ggtcgatgcc gtctatcagg cgattaaccg catcactgac tataacgtcg
     1801 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     1861 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     1921 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgc gccgcagaag
     1981 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     2041 gtcgaagaat taccatattg ccgtattgcc gggggacggt attggtccgg aagtgatgac
     2101 ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     2161 ccattacgat gtaggcggcg ctgccattga taaccacggg cagccgctgc cgcctgcgac
     2221 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     2281 ggaacattta ccaccggacc agcaaccaga acgcggcgca ctgttgcctt tgcgtaagca
     2341 cttcaaatta ttcagcaacc tgcgtccggc aaaactgtat caggggctgg aagcattctg
     2401 tccgctgcgt gctgacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     2461 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     2521 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     2581 atctgcccgc aagcgtcgcc acaaagtcac ctcaatcgac aaagccaacg tgctgcaatc
     2641 ctctatttta tggcgggaga tcgttaacga gatcgccacg gaatacccgg atgtcgaact
     2701 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     2761 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     2821 tggctcgatg ggcatgttgc cttccgccag cctgaacgaa caaggttttg gtctgtatga
     2881 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcgca
     2941 aattctgtcg ctggcattgc tgctgcgcta cagcctggat gccgatgatg cggcttccgc
     3001 cattgaacgc gccattaacc gcgcattaga agaaggcatt cgcaccgggg atttagcccg
     3061 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     3121 aggggtgtaa tcatggctaa gacgttatac gaaaaattgt tcgacgcgca cgtagtgtat
     3181 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     3241 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     3301 accttcgcca ccatggatca caacgtctct acccagacga aagacattaa tgcctgtggt
     3361 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgaa
     3421 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     3481 gtgaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac tcacggcgcg
     3541 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacgcaa
     3601 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     3661 ggcattaccg caaaagatat cgtgctggca attatcggta aaaccggtag cgcaggcggc
     3721 accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg atttaagcat ggaaggtcgg
     3781 atgaccctgt gcaatatggc aatcgaaatg ggcgcgaaag ccggtctggt tgcgccggac
     3841 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     3901 gacgccgttg cctactggaa aaccctgcaa accgacgaag acgcaacgtt cgataccgtt
     3961 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tccaggccag
     4021 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgc
     4081 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     4141 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     4201 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     4261 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggacaa aatctttatt
     4321 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     4381 cggctgaatc cgggcgaacg ttgtgcctcc accagcaacc gtaactttga aggccgccag
     4441 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgccgc ggcggtgacc
     4501 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     4561 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     4621 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     4681 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     4741 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     4801 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     4861 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
     4921 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
     4981 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
     5041 cttccgccgc cactgcatga tgaacggtct ggacagtatt gggcttacct tgcagcacga
     5101 cgacgccatt gccgcttatg aagcaaaaca acctgcgttt atgaattaat ccccttgccc
     5161 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
     5221 ttaaaaataa tgcgacaaca gaaataaccg caattaccca gtagacagca aagtgccccc
     5281 aactttgtgc aattgctccc tgaataacgc cagccagaat taccccggta gaaatactgt
     5341 tagtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
     5401 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
     5461 ccatacggct atgaaagaaa atcaatccgg tgtaaaacag tactcctgcc gccactgcta
     5521 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
     5581 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
     5641 actcgctact gatccacaac ggcatatcaa taatgtacat ggtgttgcag gtccacatta
     5701 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagccacct tgcattgata
     5761 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
     5821 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat caccgtaaag ccgtaattca
     5881 acgccagcat aaaggccaac ggtggaccga taacccatgc cagagaaagc tgcgcacgca
     5941 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcatattcc cgcgccagag
     6001 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
     6061 gcgtcagata atgacgatta aatgcaaaca atagcgcatt gccgatagcc atcaaacagc
     6121 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
     6181 ttacgccgat cccagcaata gcattcaccg tataaaagag gccgatccag aaaggttgcg
     6241 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
     6301 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
     6361 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
     6421 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
     6481 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
     6541 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
     6601 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
     6661 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
     6721 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
     6781 actggcagcg ttattgagct gctcgcgtcg tcatatgcgc accctgctca acaccatgca
     6841 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgt ggtaaacgct cgcgtctgac
     6901 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
     6961 tcgtatcgac caactagtgc agttagttgg cgacaaagcg actgtgcggc aaatgctggt
     7021 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
     7081 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
     7141 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
     7201 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
     7261 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
     7321 caatacgctg ccgctctatt cgcatattgc ggacattgtg tcgccgacgc cctggacgct
     7381 ggatatccat ctcacgcaac cggaccgctg gttaccgtta ctgctggggc aagttccggc
     7441 gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt gccagccatc ccatcggcac
     7501 cggtccgtat gcggtgatcc gcaacaccac caatcaactg aaaattcagg cattcgatga
     7561 cttcttcggt taccgggcat taatcgatga agttaacgtc tgggttctgc cggaaattgc
     7621 cgacgagcca gctggaggtc tgatgctaaa aggtccacag ggcgaggaaa aagagattga
     7681 aagccgcctg gaggaaggtt gctactattt actattcgac agccgcaccc atcgcggggc
     7741 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
     7801 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
     7861 ccatgcccgc accataaaga gcgaaaaacc ggctggtctg gaaagcctca ccctgacctt
     7921 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
     7981 ccaccaggtc acgctggaaa tcaaagagat cgactacgat cagtggcatg aaggagagat
     8041 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
     8101 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
     8161 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
     8221 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
     8281 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
     8341 gccggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
     8401 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
     8461 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
     8521 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
     8581 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
     8641 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
     8701 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
     8761 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
     8821 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
     8881 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
     8941 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgcaccagta
     9001 cgccggggct gggcctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
     9061 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
     9121 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
     9181 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
     9241 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
     9301 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
     9361 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
     9421 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
     9481 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
     9541 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
     9601 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
     9661 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
     9721 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
     9781 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
     9841 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggtat
     9901 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
     9961 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    10021 ttttttccgc ttcgtcgaat ggccgtggtt acggcgacaa atcccgccgg ttgctgcact
    10081 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcactgggcg gcggtccgca
    10141 ggcgaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    10201 cgcggcgatg ctggcgctga tccagatggt gtgttgcctt gggctggtac tgttgagtca
    10261 gcgattgagt aaggccattg cgccaggcac cacgctgctg caaggctggc gcgacccgga
    10321 cgatcgtctg catagccgca tttgcgacac ggtgttaatt gtgctggcgc tgctgctgtt
    10381 gctgccaccg ttactggcgg tgatcgtcga tggcgtaaat cgccagttgc cggaagtgct
    10441 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    10501 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    10561 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    10621 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacaccattg gcctgccgca
    10681 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    10741 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    10801 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    10861 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    10921 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    10981 tcgcagccag gacggtgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    11041 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    11101 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    11161 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gatcgccggt tttctgacgc
    11221 cagccagcgg ttcgctgact atcgacggcg ttgatcacac cacgacgccg ccgtcacgcc
    11281 gtccggtgtc gatgctgttt caggaaaaca acctgttcag tcacctgacg gtcgcgcaga
    11341 acatcgggct ggggctaaat ccgggattga aactgaacgc ggcacagcag gagaaaatgc
    11401 acgctatcgc ccgccagatg gggattgata atttaatggc gcggttacct ggcgagcttt
    11461 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    11521 tattgctcga tgaaccgttc tctgcgctcg atccggcgct acgtcaggag atgctgacgc
    11581 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    11641 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    11701 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    11761 cgggttagta tgcactgtcg gatgcggcgt gaacgcctta tccgacctac gggtagtgcg
    11821 ttttgtaggc ctgataagac gcggtcagcg tcgcatcagg caacccgcac aagactatct
    11881 cctaaccccc aaccacttta cgcaaaatat cgatatacat cggcatcaac gggtggcgaa
    11941 ttaacaccac cagcgccacc acgccgatgg cagaaattaa cggcgtcagc cacaacaaac
    12001 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    12061 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    12121 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    12181 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    12241 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    12301 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    12361 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    12421 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccag gcgtgccaga
    12481 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    12541 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    12601 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    12661 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    12721 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    12781 cggaactcgc tcgggctggc cccggtgcat tttttaaata ctcgcgagaa atagagttga
    12841 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    12901 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    12961 tggcggaaaa gatgtgacag acgcgacggt gacaagcaaa catgctgtgc gacgctggcg
    13021 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
    13081 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    13141 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    13201 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaaaccc
    13261 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaagcgcg cggacgaaag
    13321 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
    13381 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
    13441 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    13501 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    13561 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    13621 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    13681 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    13741 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    13801 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    13861 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    13921 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    13981 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    14041 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    14101 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    14161 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacacaa ccggctcgac
    14221 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    14281 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    14341 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctacattg gcggtattta
    14401 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca ccgccgtggc
    14461 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    14521 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataagt cgctatggca
    14581 cgaaagctgg ggtggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    14641 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    14701 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    14761 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    14821 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    14881 ggcagtgaaa ggtatttgcg gtcaggttga tggcagcgtg gtacctggat ttatcggtct
    14941 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    15001 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    15061 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    15121 gccggtggtg ctcgactggt ttaacggccg ccgcacaccg aacgctaacc aacgcctgaa
    15181 aggggtgatt accgatctta acctcgctac cgacgctccg ctgctgttcg gcggtttgat
    15241 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    15301 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    15361 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    15421 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    15481 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    15541 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    15601 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    15661 tgacgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    15721 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    15781 aagcgaaact gccctgcaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    15841 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    15901 acaccttctc cccagccaaa atgtggatca acggccttac catgctcaac aaaccgttgc
    15961 tgcaattcca cacccagttt aacgcggcgc tgccgtggga cagtatcgat atggacttta
    16021 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    16081 gtcagcaaca tgccgtggtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    16141 gctcctggat gcgccaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgtt
    16201 ttggcgataa catgcgtgaa gtagcggtca ccgatggtga taaagttgcc gcacagatca
    16261 agttcggttt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca
    16321 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    16381 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    16441 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    16501 atttgcacgg tctgaaacag cttccaggtc tggccgtaca gcgtctgatg cagcagggtt
    16561 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    16621 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    16681 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    16741 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    16801 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    16861 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    16921 aactgccggt ggcgaatgcg ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    16981 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    17041 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    17101 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    17161 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    17221 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattgtttt ccccctcgcc
    17281 cctttgggga gagggccggg gtgaggggaa acgacctctc aggagtcaac atgttagaag
    17341 atctcaaacg ccaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc
    17401 tcacatgggg caacgtcagc gccgttgatc gcgagcgcgg cgtctttgtg atcaaacctt
    17461 ccggcgtcga ttacagcgtc atgaccgctg acgatatggt cgtggttagc atcgaaaccg
    17521 gtgaagtggt tgaaggtacg aaaaagccct cctccgacac gccaactcac cggctgctct
    17581 atcaggcatt cccctccatt ggcggcattg tgcatacgca ctcgcgccac gccaccatct
    17641 gggcgctgtc gggtcagtcg attccagcaa tcggcaccac ccacgccgac tatttctacg
    17701 gcaccattcc ctgcacccgc aaaatgaccg acgcagaaat caacggcgaa tatgagtggg
    17761 aaaccggtaa cgtcatcgta gaaacctttg aaaaacaggg tatcgatgca gcgcaaatgc
    17821 ccggcgttct ggtccattcc cacggcccgt ttgcatgggg caaaaatgcc gaagatgcgg
    17881 tgcataacgc catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag
    17941 cgccgcagtt accggatatg cagcaaacgc tgctggataa acactatctg cgtaagcatg
    18001 gcgcgaaggc atattacggg cagtaatgac tgtataaaac cacagccaat caaacgaaac
    18061 caggctatac tcaagcctgg ttttttgatg gattttcagc gtggcgcagg caggttttat
    18121 cttaacccga cactggcggg acaccccgca agggacagaa gtctccttct ggctggcgac
    18181 ggacaacggg ccgttgcagg ttacgcttgc accgcaagag tccgtggcgt ttattcccgc
    18241 cgatcaggtt ccccgcgctc agcatatttt gcagggtgaa caaggctttc gcctgacacc
    18301 gctggcgtta aaggattttc accgccagcc ggtgtatggc ctttactgtc gcgcccatcg
    18361 ccaattgatg aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga
    18421 tgtgcgcccg ccagaacgct atctgatgga gcggtttatc acctcaccgg tgtgggtcga
    18481 gggtgatatg cacaatggca ctatcgttaa tgcccgtctg aaaccgcatc ccgactatcg
    18541 tccgccgctc aagtgggttt ctatagatat tgaaaccacc cgccacggtg agctgtactg
    18601 catcggcctg gaaggctgcg ggcagcgcat cgtttatatg ctggggccgg agaatggcga
    18661 cgcctcctcg cttgatttcg aactggaata cgtcgccagc cgcccgctgc tgctggaaaa
    18721 actcaacgcc tggtttgcaa accacgatcc cgatgtgatc atcggctgga acgtggtgca
    18781 gttcgatctg cgaatgctgc aaaaacatgc cgagcgttac cgtcttccgc tgcatcttgg
    18841 gcgcgataat agcgagctgg agtggcgcga gcacggcttt aaaaacggcg tcttttttgc
    18901 ccaggctaaa ggtcggctaa ttatcgacgg tatcgaggcg ctgaaatccg cgttctggaa
    18961 tttctcttca ttctcgctgg aaactgtcgc tcaggagcta ttaggcgaag gaaaatctat
    19021 cgataacccg tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataaacctgc
    19081 gctggcaact tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga
    19141 aatcatgcca tttttactcg aacgagcaac ggtgaacggc ctgccggtgg atcggcacgg
    19201 cggttcggtg gcagcgtttg gtcatctcta ttttccgcga atgcatcgcg ctggttatgt
    19261 cgcgcctaat ctcggcgaag tgccaccgca cgccagccct ggcggctacg tgatggattc
    19321 acggccaggg ctttatgatt cagtgctggt gctggactat aaaagcctgt acccgtcgat
    19381 catccgcacc tttctgattg atcccgtcgg gctggttgaa ggcatggcgc aacctgatcc
    19441 agagcacagt accgaaggtt ttctcgatgc ctggttctca cgggagaaac attgcctgcc
    19501 ggagattgtg actaacatct ggcacgggcg cgatgaagcc aaacgtcagg gtaataagcc
    19561 gctttcgcag gcgctgaaga tcattatgaa tgccttttat ggcgtgctcg gcaccaccgc
    19621 ctgccgcttc ttcgatccgc gcctggcatc gtcgatcacc atgcgtggtc atcagatcat
    19681 gcggcaaacc aaagcattga ttgaagcgca gggctacgac gttatctacg gagataccga
    19741 ctcgactttt gtctggctaa aaggtgcaca ttcggaagaa gaggcggcga aaatcggtcg
    19801 tgcactggtg cagcacgtta acgcctggtg ggcggaaacg ctgcaaaaac aacggctgac
    19861 cagcgcatta gaactggagt atgaaactca tttctgccgt tttcttatgc caaccattcg
    19921 cggagctgat accggcagta aaaagcgcta cgccgggctg attcaggagg gcgacaagca
    19981 gcggatggtg tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca
    20041 gtttcagcag gagctgtacc tgcgtatctt ccgcaacgag ccatatcagg agtacgtccg
    20101 ccaaaccatc gacaaactga tggcgggtga actggatgcg cggctggttt accgtaaacg
    20161 ccttcgccgt ccgctgagcg aatatcagcg taatgttccg cctcatgtac gcgccgctcg
    20221 ccttgccgat gaagaaaatc aaaagcgcgg tcgccccttg caatatcaga atcgcggcac
    20281 cattaagtac gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccact
    20341 ggattacgaa cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt
    20401 tattgaggat aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa
    20461 aagagttcgc cagataccat tttgatgcgt gacgaatgct ttgccatcca gtaccatagc
    20521 gccctttcca ttcctggacc tgaataacac cactacctca taagcacggt agcgggtggt
    20581 tattgcctgc aattaaagat atagagccga acacatatgc cttttacact tggtcaacgc
    20641 tggatcagcg atacagaaag cgaattggga cttggaaccg ttgtcgcggt ggatgcgcga
    20701 actgtcactt tacttttccc atctactggt gaaaaccgtc tgtacgcacg cagtgattcc
    20761 cccgtgaccc gcgtgatgtt caaccctggt gataccatta ccagccatga cggctggcag
    20821 atgcaagttg aagaagtaaa agaagaaaat ggcttgctga cctatatcgg tactcgcctg
    20881 gatactgaag agtccggcgt agccctgcgt gaagttttcc ttgatagcaa actggtgttc
    20941 agcaaaccgc aggaccgtct gtttgccggg cagattgacc gtatggaccg ctttgcgctg
    21001 cgttatcgcg cgcgtaagta ttccagcgaa cagttccgta tgccgtacag cggcctgcgc
    21061 ggtcagcgta ccagcctgat tccgcatcag ctcaacatcg ctcatgatgt tggtcgccgc
    21121 cacgcgccgc gcgtcctgct ggctgacgaa gtgggtttag ggaaaaccat tgaagccggg
    21181 atgatcctgc atcagcaact gctctctggc gctgctgaac gtgtgctaat tatcgtcccg
    21241 gaaaccttac agcatcagtg gctggtagaa atgctgcgcc gtttcaacct gcgctttgcg
    21301 ctatttgatg atgagcgtta tgccgaagct cagcacgatg cttacaaccc gttcgacacc
    21361 gaacagctgg tgatttgctc gctggatttt gcccgtcgta gcaaacagcg cctggaacat
    21421 ctctgtgaag ccgaatggga cctgctggtg gtcgatgaag cgcatcacct ggtgtggagc
    21481 gaagatgcgc caagccgtga atatcaggcc attgaacaac tggcagagca cgtgccgggc
    21541 gttctgctgc tgaccgcgac cccggaacag ctggggatgg aaagccactt cgcccgtctg
    21601 cgtctgctgg acccgaaccg tttccacgat tttgcgcagt tcgttgaaga gcagaaaaat
    21661 tatcgtccgg ttgcggacgc cgttgccatg ctgctggcag gtaacaaact gagcaatgac
    21721 gaactgaaca tgctcggcga gatgatcggc gagcaggata tcgagccgct gttgcaggca
    21781 gcaaacagcg acagcgaaga tgcccagagc gcccgtcagg agctggtttc gatgctgatg
    21841 gatcgccacg gcaccagccg cgtgctgttc cgtaacaccc gtaacggtgt gaaaggcttc
    21901 ccgaaacgcg agctgcacac cattaagctg ccgctgccga cgcagtatca gacggctatt
    21961 aaagtctccg gcattatggg cgcacgcaaa agtgcggaag atcgcgctcg cgatatgctc
    22021 tacccggagc gtatttatca ggaatttgaa ggtgataacg ccacctggtg gaacttcgat
    22081 ccgcgcgttg agtggctgat gggctacctg accagccatc gctctcagaa agtgctggtg
    22141 atctgcgcta aagccgctac cgcgctgcaa ctggagcagg tactgcgcga acgtgaaggt
    22201 atccgcgccg cggtgttcca cgaaggcatg tcgattattg agcgtgaccg cgctgccgcc
    22261 tggtttgccg aagaagacac cggcgcacag gtactgctgt gctcggaaat cggttctgaa
    22321 ggacgtaact tccagttcgc cagccacatg gtaatgtttg acctgccatt caacccggat
    22381 ctgctggagc agcgtattgg tcgtctggat cgtatcggtc aggcgcacga tattcagatc
    22441 catgtgcctt atctggaaaa aactgctcag tcggtgctgg tgcgctggta tcacgaaggt
    22501 ctggatgcat tcgagcacac ctgcccgacc ggacgcacta tttacgatag cgtatacaac
    22561 gatctgatta actatctggc ttcaccggat caaaccgaag gctttgacga tctgatcaaa
    22621 aactgccgcg agcagcatga agcgctgaaa gcacagctgg aacagggtcg tgaccgcctg
    22681 ctggaaatcc actccaacgg tggcgaaaaa gcccaggcgc tggcagaaag cattgaagag
    22741 caggatgacg ataccaacct gatcgccttc gccatgaacc tgttcgatat tatcggtatc
    22801 aatcaggacg atcgcggcga caacatgatc gtgctgacgc cgtccgatca tatgctggtg
    22861 ccggacttcc ctggcctgtc ggaagatggc atcaccatca cctttgatcg tgaagtggcg
    22921 ctggcgcgtg aagatgcgca gtttattacc tgggagcatc cgctgatccg caacggtctg
    22981 gatctgatcc tttctggcga tactggtagc agcacgattt cactgttaaa aaacaaagcg
    23041 ttgccggtag gtacgctgtt ggtggaactg atttatgtcg ttgaagccca ggctccgaag
    23101 cagttgcagc tcaaccgctt cctgccaccg acgccggtac gtatgctgct ggataaaaac
    23161 ggcaacaacc tggcggcgca ggtagaattt gaaaccttta accgtcagct taacgcggtt
    23221 aaccgtcaca ccggcagcaa actggttaac gccgtgcagc aggatgttca cgctatcctt
    23281 cagctgggtg aagcgcagat cgagaaatct gcccgtgcat tgattgatgc cgcacgtaac
    23341 gaagccgacg aaaaactgtc tgccgagctg tctcgtctgg aagcgctgcg cgcagtgaac
    23401 ccgaacattc gtgacgacga actgaccgcc attgagagca accgtaagca ggtaatggaa
    23461 agcctggatc aggcaggctg gcgtctggat gccctgcgtt tgatcgttgt gacgcatcag
    23521 taacggagcc gaaaatgggg atggaaaact acaatccacc gcaggaaccc tggttggtta
    23581 tcctgtatca ggatgaccac attatggtgg tcaacaagcc gagcggtttg ttgtcagtgc
    23641 cgggtcgtct ggaagagcac aaagatagcg tgatgacgcg cattcagcgt gattatccgc
    23701 aggcagaatc ggtgcatcgt ctggatatgg ctaccagcgg cgtgattgta gtggcgctga
    23761 ccaaagccgc ggagcgggag ttaaaacgcc agttccgcga gcgcgagccg aaaaagcaat
    23821 atgtggcccg cgtctggggg catccatccc ctgcggaagg tctggtggat ctgccgctga
    23881 tttgcgactg gccaaaccgc ccgaaacaga aagtctgtta cgaaacgggc aagcctgcgc
    23941 agacggaata tgaagtggtg gagtatgcgg cggataacac ggcaagagtg gtgttaaaac
    24001 cgattaccgg gcgttcgcat caattgcgtg tgcatatgct ggcgctgggt catccgattc
    24061 tcggcgatcg tttttatgca tcaccagaag cgagagcgat ggcaccgcgt ttgttgctac
    24121 atgcagagat gttgacgatt acccatccgg cgtatggcaa tagtatgacg tttaaagcgc
    24181 cagcggattt ttaatcgtca cgctggcgca gttcggtagg cggataaggc gttcacgccg
    24241 catccggcaa ttgtgcgccg attgcctgat gcgacgcggg ccgcgtctta tcaggcctac
    24301 agtttaaaac ttatttaaac cctttctgct gctttatcag ctcatatgcc tgctgaattt
    24361 cctgcgcttt ctgcttcgcc atctccatca tctcaggcgg caaacctttc gccaccagct
    24421 tatcgggatg gtgttcactc atcagcttac ggtaggcacg tttgatggtg gtcgcatcat
    24481 ccgtcggctt cacgcccagc acattacagg catcttccag cgttgggcca cgctgcgctt
    24541 gctgccagtt accaccgcca gtttgctgct gataaccgcc gccaaactgt gcaccgccct
    24601 gcatcatgcg caaaaactgg tcaaactgag cgcgggagat ccctaattct tctgcaatga
    24661 catacagcac cgcccgttca ttcgggtgca gtgaaccatc agcaaacgcc gcctgaatct
    24721 ggatctccag aaacatacga attaagtcaa aacgaccaaa gcagacactg cgaaactggc
    24781 gcatcttttc gcgcagcggg taattgtctg attttcccac ccggaacgcg ttttgcgccg
    24841 cagtacggga agcgccatga aggttcattc ggtccatcag ctggctggcg atatgaatat
    24901 cagcctccgt gacgcgacct ttggatttgg ttaaatgccc cataacttca aaagtggtgg
    24961 caaaaaacag cgcctgacgc tcacgctggt tggcgaacca cgccatttta cggctacggg
    25021 ctttatcaaa catatggcca attaacaggc ctaacactac gccccaaaag ccgccgccca
    25081 tcagtaaggc cacggccacg ccaatgattt ttccccaata ctgcatatat tccccaaatc
    25141 gacacacgga tatcagggct atctcccaca atataaaggt gcttttaccg ttttccggct
    25201 gcggtcaatt gtgggacatc gcctataatt tgcattatca tacctgtcat tcaatgccgt
    25261 gcctaacacc actgacgcta ttcggacagg attaacacta gcgtagagat gacgagtacg
    25321 ttagtctctg accgtttgtc acgcgcaacg ttaccgatga tggaacaata aaatcaacgt
    25381 atgaaaaaac gtatccccac tctcctggcc accatgattg ccaccgccct ttatagtcaa
    25441 cagggactgg cagccgatct cgcctcacag tgcatgttgg gcgtgccaag ctatgaccgt
    25501 cctctggtac agggcgatac caatgactta cccgtgacta tcaatgctga ccacgcgaaa
    25561 ggggactacc cggatgacgc cgtgtttact ggcagcgtgg atatcatgca gggtaacagc
    25621 cgtctgcagg ccgacgaagt gcagctccat caaaaagagg caccaggaca accggagccg
    25681 gtacgtaccg ttgatgcgct cggtaatgtc cattacgacg ataaccaggt gatcctcaaa
    25741 gggccgaaag gctgggcgaa tctgaacacc aaagatacca acgtctggaa aggtgattac
    25801 cagatggtgg gtcgccaggg ccgcggtaaa gcggacctga tgaaacaacg tggtgaaaac
    25861 cgctatacca ttctggataa cggtagcttt acctcctgtc tgccgggttc tgacacctgg
    25921 agcgtggtag gtagcgaaat tattcatgac cgcgaagaac aagttgcgga gatctggaac
    25981 gcccgcttta aggtgggtcc ggtaccgatc ttttatagcc cctatttgca gttgccggtg
    26041 ggtgacaaac gtcgctctgg tttcttgatc ccgaacgcca agtacaccac caccaactac
    26101 tttgagttct acctgccata ttactggaac atcgcgccaa atatggatgc caccatcacg
    26161 ccgcattata tgcatcgtcg tggcaacatc atgtgggaga acgaattccg ctacctctcc
    26221 caggcgggcg ctggcttgat ggaactggac tatctgcctt cagataaagt ctatgaagat
    26281 gaacacccga acgatgacag ttcacgtcgt tggttattct actggaacca ctccggggtc
    26341 atggatcaag tgtggcgttt caacgtcgac tacaccaagg tcagcgatcc tagctacttc
    26401 aatgatttcg ataacaagta cggttccagt actgacggct acgcaacgca aaaattcagc
    26461 gttggctatg cggtgcaaaa cttcaatgcc accgtttcaa ccaagcagtt ccaggttttc
    26521 agcgaacaga acaccagtag ctactcggca gagccgcagt tagacgttaa ttactaccag
    26581 aatgatgttg gtccgtttga tacgcgtatt tacggccagg cagtgcactt tgttaacacc
    26641 agagacgaca tgcctgaagc aacccgtgtt cacctggaac cgaccatcaa tttgccgctc
    26701 tctaataact ggggcagcat caataccgaa gcgaagttgc tggcaaccca ttatcagcaa
    26761 accaatcttg actggtataa ctccagaaac acgaccaagc tggacgaatc cgttaaccgc
    26821 gtaatgccgc aattcaaagt tgacggcaaa atggtctttg aacgcgatat ggaaatgctg
    26881 gctccgggtt atacccaaac gctggaaccg cgcgcgcagt atttgtacgt gccgtatcgc
    26941 gatcagagcg acatctataa ctacgactcg tctctgctgc aatctgacta ctctggcctg
    27001 ttccgggacc ggacttacgg cggtcttgac cgtattgcct ccgctaacca ggtgacgacc
    27061 ggtgtcacat ctcgcatata tgatgatgct gccgttgaac gttttaatat ttccgttggt
    27121 caaatctact atttcacgga gtctcgcact ggcgatgaca acataacatg ggagaatgac
    27181 gacaaaacgg gttcactggt gtgggcaggc gatacttact ggcgtatctc cgagcgttgg
    27241 ggattgcgtg gcgggattca gtacgataca cgtctggata acgtagcgac cagtaactcc
    27301 agcattgaat accgtcggga tgaagaccgt ctggtacagc tgaattaccg ttacgccagc
    27361 ccggaatata ttcaggctac gctgcctaag tactattcca ctgctgagca atataagaat
    27421 ggtatttcgc aggtaggtgc tgtcgccagc tggccaattg ccgatcgttg gtccattgtt
    27481 ggggcctact actacgacac caatgcgaac aagcaagccg actctatgtt aggtgtgcaa
    27541 tacagctcct gctgctatgc aattcgcgtc ggttacgagc ggaagctgaa cggttgggat
    27601 aacgataaac aacatgcggt atatgacaac gcaatcggct ttaacatcga acttcgcggc
    27661 ctgagctcca actacggtct gggtacgcaa gagatgctgc gttcgaacat tctgccgtat
    27721 caaaacactt tgtgatttgt tgatttacca cgtaatccgc agtgcggtta attgaaatgg
    27781 aaaaagtatg aagaactgga aaacgctgct tctcggtatc gccatgatcg cgaataccag
    27841 tttcgctgcc ccccaggtag tcgataaagt cgcagccgtc gtcaataacg gcgtcgtgct
    27901 ggaaagcgac gttgatggat taatgcagtc ggtaaaactg aacgctgctc aggcaaggca
    27961 gcaacttcct gatgacgcga cgctgcgcca ccaaatcatg gaacgtttga tcatggatca
    28021 aatcatcctg cagatggggc agaaaatggg agtgaaaatc tccgatgagc agctggatca
    28081 ggcgattgct aacatcgcga aacagaacaa catgacgctg gatcagatgc gcagccgtct
    28141 ggcttacgat ggactgaact acaacaccta tcgtaaccag atccgcaaag agatgattat
    28201 ctctgaagtg cgtaacaacg aggtgcgtcg tcgcatcacc atcctgccgc aggaagtcga
    28261 atccctggcg cagcaggtgg gtaaccaaaa cgacgccagc actgagctga acctgagcca
    28321 catcctgatc ccgctgccgg aaaacccgac ctctgatcag gtgaacgaag cggaaagcca
    28381 ggcgcgcgcc attgtcgatc aggcgcgtaa cggcgctgat ttcggtaagc tggcgattgc
    28441 tcattctgcc gaccagcagg cgctgaacgg cggccagatg ggctggggcc gtattcagga
    28501 gttgcccggg atcttcgccc aggcattaag caccgcgaag aaaggcgaca ttgttggccc
    28561 gattcgttcc ggcgttggct tccatattct gaaagttaac gacctgcgcg gcgaaagcaa
    28621 aaatatctcg gtgaccgaag ttcatgctcg ccatattctg ctgaaaccgt cgccgatcat
    28681 gactgacgaa caggcccgtg tgaaactgga acagattgct gctgatatca agagtggtaa
    28741 aacgactttt gctgccgcag cgaaagagtt ctctcaggat ccaggctctg ctaaccaggg
    28801 cggtgatctc ggctgggcta caccagatat tttcgatccg gccttccgtg acgccctgac
    28861 tcgcctgaac aaaggtcaaa tgagtgcacc ggttcactct tcattcggct ggcatttaat
    28921 cgaactgctg gatacccgta atgtcgataa aaccgacgct gcgcagaaag atcgtgcata
    28981 ccgcatgctg atgaaccgta agttctcgga agaagcagca agctggatgc aggaacaacg
    29041 tgccagcgcc tacgttaaaa tcctgagcaa ctaatggtta aaacccaacg tgttgtgatc
    29101 actcccggcg agcccgccgg gattggcccg gacttagttg tccagcttgc acagcgtgag
    29161 tggccggtcg aactggttgt ttgtgccgat gccactctcc ttaccgaccg ggcagcgatg
    29221 ctcggtttgc cgctcaccct ccgcccttat tcccccaact cccctgcaca accgcaaact
    29281 acgggcacat taacgctact tcctgtcgcg ctacgtgaat ctgtcactgc ggggcagtta
    29341 gcgattgaaa atggacatta cgtggtggag acgctggcgc gagcgtgtga tggctgtctg
    29401 aacggtgaat ttgctgcgct gatcacaggc cccgtgcata aaggcgtcat taacgacgca
    29461 ggcattccgt ttaccggtca taccgagttt ttcgaagagc gttcgcaggc gaaaaaagtg
    29521 gtgatgatgc tggcgaccga agaacttcgc gtggcgctgg caacgacgca tttaccgctg
    29581 cgcgatatcg cagatgctat cacccctgca cttttgcacg aagtgattgc tattttgcat
    29641 cacgatttgc gtacaaaatt tggtattgcc gaaccgcgca ttctggtctg cgggctgaat
    29701 ccgcacgcgg gcgaaggcgg tcatatgggt acggaagaga tagacaccat tattccggtg
    29761 ctcgacgagc tgcgggcgca ggggatgaaa ctcaacgggc cgctgcctgc cgataccctg
    29821 tttcagccga aatatctcga taacgccgac gccgtgctgg cgatgtacca cgatcagggt
    29881 cttcccgtgc taaaatacca gggcttcggg cgcggtgtga acattacgct gggcctgccc
    29941 tttattcgca catcagtgga ccacggcacc gcgcttgaac tggcgggacg tggcgaagcc
    30001 gatgtcggca gttttattac ggcgcttaat ctcgccatca aaatgattgt taacacccaa
    30061 tgaataatcg agtccaccag ggccacttag cccgtaaacg cttcgggcaa aactttctca
    30121 acgatcagtt cgtgatcgac agcattgttt ctgccattaa cccgcaaaag ggccaggcaa
    30181 tggtcgaaat cggccccggt ctggcggcat tgaccgagcc ggtcggcgaa cgtctggacc
    30241 agctgacggt catcgaactt gaccgcgatc tggcggcacg tctgcaaacg catccattct
    30301 taggcccgaa actgacgatt tatcagcagg atgcgatgac ctttaacttt ggcgaactgg
    30361 ccgagaaaat gggtcagccg ctgcgtgttt tcggcaacct gccttataac atctccacgc
    30421 cgttgatgtt ccatctgttt agctatactg atgccattgc cgacatgcac tttatgttgc
    30481 aaaaagaggt ggtgaatcgt ctggttgcag gaccgaacag caaagcgtat ggtcgattaa
    30541 gcgtcatggc gcaatactat tgcaacgtga tcccggtact ggaagttccg ccgtcagcct
    30601 ttacaccacc acccaaagtg gattccgccg tcgtgcgcct ggttcctcat gcaacgatgc
    30661 ctcacccggt taaagatgtt cgtgtgttga gccgcatcac caccgaagcc tttaaccagc
    30721 gtcgtaaaac cattcgtaac agcctcggca acctgtttag cgtcgaggtg ttaacgggaa
    30781 tggggatcga cccggcgatg cgagcggaaa atatctctgt cgcgcaatat tgccagatgg
    30841 cgaactatct ggcggagaac gcgcctttgc aggagagtta acgatgatca attcgccccg
    30901 agtgtgtatt caggttcaaa gcgtctacat tgaggcacaa tcttcacctg ataatgaacg
    30961 ttacgttttt gcttataccg taaccatacg caatctgggg cgagcgccag tgcagttgtt
    31021 ggggcgttac tggctgatca ccaatggcaa tggccgtgaa accgaagtcc agggcgaagg
    31081 agtggttggc gtccagccac ttatcgcgcc tggcgaagag taccagtaca ccagcggtgc
    31141 aatcattgaa accccgctgg gcaccatgca gggtcactac gaaatgatcg atgaaaatgg
    31201 cgtccctttc agcatcgaca ttcccgtatt ccgactcgcc gttcccacac tcattcatta
    31261 aaagaatatg gcgacatacc ttattggcga cgttcatggt tgttacgatg aactgatcgc
    31321 attgctgcat aaagtagaat ttacccctgg gaaagatacc ctctggctga cgggcgatct
    31381 ggtcgcgcgc gggccgggtt cgctggatgt tctgcgctat gtgaaatcct taggcgacag
    31441 cgtacgtctg gtgctgggca atcacgatct gcatctgctg gcggtatttg ccgggatcag
    31501 ccgcaataaa ccgaaagatc gcctgacacc gctgctggaa gcgccggatg ccgacgagct
    31561 gcttaactgg ctgcgtcgcc agcctttgct gcaaatcgac gaagagaaaa agttggtgat
    31621 ggcccacgca gggatcacgc cgcagtggga tctacagacc gccaaagagt gcgcgcgcga
    31681 tgtagaagcg gtgctatcga gtgactccta tcccttcttt ctcgacgcta tgtacggcga
    31741 tatgccaaat aactggtcac cggaattgcg ggggctggga agactgcgtt ttatcaccaa
    31801 cgcctttacc cgtatgcgtt tttgctttcc aaacggtcaa ctggatatgt acagcaaaga
    31861 atcgccggaa gaagcccctg ccccactgaa accgtggttt gcgattcctg gccctgtcgc
    31921 tgaagaatac agcatcgcct ttggtcactg ggcatcgctg gaaggcaaag gtacgccgga
    31981 aggtatttac gcgctggata ccggctgctg ctggggtggg tcattaacct gcctgcgctg
    32041 ggaagataag cagtattttg tccagccgtc gaaccggcat aaggatttgg gtgagggaga
    32101 ggcggtcgcg tcttaacgaa ttaccattca gggaggaata atggctgact attttgaaat
    32161 acgtgctgat tacaatcagc acaccatcac gatttatcag gcttacaatg atgcaatcgc
    32221 tgacgtcgcc gtacgtgacg ggcgatttgg cgcacctttt tcattcaacc gcatgacctg
    32281 gattaaaccc tcttttatgt ggatgatgga gcgtagcaac tggggactga aaaaagatca
    32341 acaacacatt ctcgcaatcc gaatcaaacg caccttcttt gatacattgc tggaacaagc
    32401 ggtgctaacc acgccggaag cccatgtgta tccacatgca ggaatttggg aaacgttatt
    32461 tgcacaagca aatgtgtatg ttcagtggga cccagaacgc agcataaatg gtaaaaaact
    32521 ggagcaccga tcattacagc ttggtatttc tcgtaacttg atcagtcaat ttaacgaaga
    32581 cgcgattgtt gctattgatg atctgacgcc gctggtgcga aagtgtcata accttttgat
    32641 aaacggcaaa acaacacagg cgaaatcctt cctgccgcca gaaaaaattt acccagttag
    32701 tgcagcagcg agaaaggctt tgggaatgaa aaattcctga gacaaatacg cacctattaa
    32761 gaggaaaata tctaagtgcg acaaatcaac accgggctta ataccagaga aattattctg
    32821 gaaatgattg acgaagcaga tgcccccccg tttgctgagc agcggacctg gcatcgcagg
    32881 cgaatcgctt ttctatgtga ttgcatcagc gttgctgtcg actacggctt cttctgttct
    32941 gtctctcagc agtcaataat gaacataacc aagatggctg aagatgtatg gataacagaa
    33001 agtaaaacga cccaactcat tgatatttgg caacaactta aacagcttta tccgccagaa
    33061 actattccct acgcccagaa gttacccact aaggaattgc ttgaactgct actttctagt
    33121 cctgacgatc ttcgaataat tgaaattcct gacatcatcg gtgatgtttt tgatgaccaa
    33181 acctggattc gattattcaa aaaacacttt gctgatgtta ttcagctacg atgtgatgtg
    33241 taacaatcca aaatatatcg ccagacgata gaccaaaaag aaagtattta gcagatactc
    33301 aacattctga cttctgtaca gaaatagcag agtttaaaaa cttaaaactt ccggtactca
    33361 tcagagaaac atccagtttc ttctgcaaat tcatttaatg ccgagatggc atcctcgttt
    33421 acttcattcc actgtagagt ggcttgaagg tttagaccta cgcaatgagc gcgactcaca
    33481 aaatcgcgct cactggtaaa atcaaacgtc ttatcgttca atttgtagtc ctggcaaaaa
    33541 attaccgccg ctccagaatc tcaaagcaat agctgtgaga gttctgcgca tcggcgtcgt
    33601 ggaattcgct gaataccgat tcccagtcat ccggctcgta atccgggaaa tgggtgtcgc
    33661 cttccacttc tgcgtcgata tgcgtcagat acagtttttg cgcttttggc aagaactgtt
    33721 cataaacgcg accgccgcca atcaccatga tttctggtac gtcaccacac gccgcgatgg
    33781 cttcatcaac cgatttcacc cacgttacgc gatcgtccgt acccggttga ctgctgagga
    33841 taatattttt gcgtcctggc agcggacgac cgatggattc ccaggtatgg cggcccataa
    33901 taacgggttt atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccacg
    33961 gcatggcgtt ttccatgccg ataacgcgat ctaccgctaa cgccgcaatc agactgatca
    34021 ttgagatttc ccgataaaaa aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga
    34081 ctggtgcgag gatgatgttg aggaaaattt tatattctgc tggcgagtcc acgctctctc
    34141 cctggactcg ccacattaca atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta
    34201 cgtcactctt tattaggata agggtttcgt ttccggttca tccgccatgt tgccggtatg
    34261 tttaccttct tctgttccct gccagccatg tcgctgaatt aatgacagat gttcgcggtc
    34321 ctcggtaatg atctcactta acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt
    34381 ggtgtcattc tcaaccattg ccatctcttc caccatctga atattaaagc ggcggaacac
    34441 atcggcacgt tctcgcgctt catacggccc cagacctaaa ctttccagcg ccagacgccc
    34501 ggttttcagc gcaccttcaa aggtttcacg ctccggcttt tcaacgcctg cctgacgcaa
    34561 acgaatgtag tggtcgacat cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc
    34621 tttcaccatc tctgtcagtt gcaggttggt ttgcggatcg tcgatggcgt taatcagcac
    34681 ttccgctttc gccgctccgg cagattccag taaatccatc cgcgtggcat cgccataaaa
    34741 cactttcata ccaaatttac gcaaggtttc gatatggtcc ggatcgtgat cgagtaccac
    34801 cattttcacc ccgctggaga gcagtaaacg tccggtaatc tgcccaaaac gaccgaatcc
    34861 ggcgataatc acgcgcggct gttcttcgtc gatctcatcg gcttcacgcg cttcctcagt
    34921 agaagattgc tcaaggcgat tgaggatcac cagcagaatc ggcgttgctg ccatcgacag
    34981 cgccaccgcc agggtcagcg atttcgccca ctccggctcc agcacattcg ccatctgcgc
    35041 cgcgccaaat accacaaagg caaactcact gccctgccct aacaacaccg caaaccaacg
    35101 acgctgttta tttggcactt gcaacggtcg ggcaatcagc cacagcatgg cgattttgat
    35161 gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg ttttcaagca gcgtgccaaa
    35221 gtctatcgac atgccaacac cgatgaaaaa cagccccaac agcaaacctt taaatggttc
    35281 gatatcgctc tccagcgcat gacggtattc cgagcttgcc agcagtacgc ccgccagaaa
    35341 cgcgcccatc gccatcgaca agccgacctc ttccagcagc aaaccaaagc caaacacgag
    35401 gaataacgcc acggcactaa acacttcccg caagccagag cgggctacaa aacgcagcgc
    35461 cggacgcgtg acatagcgcc ccagcaatac caccagcacc agcgcacccg ccacttttaa
    35521 cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg ctcgttgcca gtagcggaat
    35581 catcgccacc agcgggatcg ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg
    35641 gcccatttgc gtcaccatca gattgcgttc attcatcgcc tgcatggcaa tcgccgttga
    35701 tgagagcgcc agcgtcatgc cgatcagttc cgcgacctgc cagcgcagcc caagtaacat
    35761 gcagaacagc cccagcaggc caccgcaaat caccatctgc aatgcgccgc cgccgaacac
    35821 tgccgcacgc agcttccata gcctttgtgg atcgagttcg aggccgataa taaacagcat
    35881 cagcaccacg ccaatctcgg caaagtgcag gatagattcg gcatcggtca ccagtcgcag
    35941 cccccacggg ccaataatgc agccggcaat caggtagcca agtaccgatc ccagaccaag
    36001 acgtaccgca atgggtacaa tcagcgccgc cgatccgagg tagatcagcg cctgaaccag
    36061 cgtatggcta tccatgatgg gcctcctgcc attccagcag acgttgctta tagtgacgcg
    36121 cctgcccttc gagggtttcg tcgtcacaaa taaaggtgca gtgcatggca aacggtggca
    36181 gccagttcag cccgcagtag attgccgtcg cctgtagcgg ctgcgacagc acatcaaagc
    36241 ccggatgcgc accaatttca aaatggcttt ccccgccgcc ggtcgtcacc gcccacagca
    36301 aatgtttgcc atgcagcgcc gtgccgccgt gaccgtaagc ccagccgtgc gagaaaactt
    36361 tatcgatcca aagtttgagg agcggtggaa tgctgtacca ctgcatcggg tgctgccaga
    36421 cgatcagatc ggcgcgagac agcgcctcct gctcggcggc aatatcgata ttgaagtcag
    36481 gatagagttg ataaagagag cgaatttcga cgccttccag cgtccttgcc tgttcaagca
    36541 tccgtttatt cgcatgggaa tgatgcggat acggatgcgc ataaattata agaatcatag
    36601 gtagcccgcg tgattacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct
    36661 tatagtcaat aatattgatt cagaaaagag agaaatctga acaaattacc ccattctctg
    36721 cccgcggatc gtcatattgc tggtctgcgc cagtgacatc cctcgagtct ccggggcaaa
    36781 cgctacggaa atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact
    36841 gataccgtaa ttattgataa agatcggcag tgcccaagtc gaaacaatgg tgccaatacg
    36901 acttaaggac ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt
    36961 aggatagagc cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc
    37021 catcactacc agccagatcc ccatatccgg gatcagcccc aaaaccgcca gcgccagcgt
    37081 catcatggca aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat
    37141 cggcggaata cagccgagca taaagaacag gctaatcacc acattcccca gtgccgcgtt
    37201 tttgccaacc cccaatccca acaaaccaac gatttgcggg ccaaaggtgt agatggcgaa
    37261 catcgggatc acctggcaag tccagatggc ggcaacaaac agaacaaaag gaaaatggcg
    37321 gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc tcttcctcga aagccaccgg
    37381 ttcgccaaac agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa
    37441 ccagcgggga gattcaggca gttcgaatcg accaatcaaa atcaacaaac aggggatcgc
    37501 cgcgctaccc agcatccagc gccagccgcc ttccacatca taaagccagt agccgaccag
    37561 atcggcgcag gtcgcgccta cataccacat ggcggcgata aagctgatgg aaaatgcccg
    37621 ctgacgggta ctggagaact cggtgatcat tgaggtggcg atgggataat ctgcaccgat
    37681 gacaatgccg ataagtaccc gcatcaccaa cagttcgacg ggggatgaaa caaacatcgt
    37741 cgccaccgat atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac
    37801 tttatcggaa atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc
    37861 gcccaacaag ccaatccagt cagcgtccag ttttagcgcc ggagtcagtt gctccagcgc
    37921 cacgccaatc attaccagta cataaccatc cagcaacgga ccaccgcttc cccacagcaa
    37981 aatgcggcgg tgaattgagg agaatttgag atcgtcaaag tttctggacg gttgcatggg
    38041 ttattcctgt ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc
    38101 cgtaacggaa ctccacacca aaggtgccgc gcgagtattc ccactgttcc agcgccgacc
    38161 ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac
    38221 tgccgtcatc ctgcttcttg tacagacctg cggggcacac tttcaccagc agctccaggg
    38281 cctgtttatc ggcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt
    38341 tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt
    38401 catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt
    38461 gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc
    38521 cagttccggg tagccgctaa acatgcgtgg gttatcaagg aaggccggta gtttctggta
    38581 catacgcata tcgcgcagcg ggccactctc aagatgctga cgatattccg cgagtttttg
    38641 cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc
    38701 ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc
    38761 atcaccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc
    38821 cggcactacg tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgccactgc
    38881 cggatgctgt ttgaaatctt ccagcatttg cggcaccgat ttttttgcgt catgcagatg
    38941 atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc
    39001 gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg
    39061 caactgaaaa cggtcttcga ttaccgactt cggtaactcg atcagttcct tcacgccaac
    39121 cgccacatcc gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt
    39181 caccccatca gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg cttctacacc
    39241 gacgactttg ccatcgcgct gtacgaggtt atcgacacga atcccggtaa ttaactgcgc
    39301 gcccgcttct tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta
    39361 agaacgctgg gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt
    39421 ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cagattcggc
    39481 gaaaccagga ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg tgacgttctt
    39541 ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac
    39601 cagtgcggca accgaaccgg caagcccagc accgacgatg atggcgtcaa agatatcttc
    39661 ggacataaga gctccctgtc agcggcaggg ccaaaatgca gccctgccag agtggatcaa
    39721 cgcgctaaag ctgcggtcag cgccggaagg atcttcacgg cgtcgccaac aatgccgtaa
    39781 tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac
    39841 gcgttagcgc caaccatgtg ctggatctgc ccggagatcc ccaccgccag gtacagttca
    39901 ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt
    39961 tctgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc
    40021 agcgcaatgt tctctttgct gccaataccg cgaccgacgc tgaccaccag gcgggctttg
    40081 tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc
    40141 ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca
    40201 tcgaacgtgc cgctgctgat ggtcagtacc gcataaggcg tggcaatgcg ttcttcgcca
    40261 atcgccagac caccgtaaac catgtgtttc actgtcgctt taccgtcctg tacgctgacg
    40321 gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat
    40381 ttgccgcgac gggtgtttgg cagcagcacc aggccgtctg cgccgtgctg gcgaatagtg
    40441 tcagccatga caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc
    40501 cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag
    40561 gtgttgattt gattagctaa agcctgcgca ccgttcatca gttccggcag acgagaaggg
    40621 gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga
    40681 ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga
    40741 tgcgctgacg ttcgcgctgt ttcggcgcgg caacctgttg ttctgaccag gctgcctctg
    40801 cgttaaaacc aatatccgcc gccgaccata cctggacggg ctttttcgcc gcgccgagaa
    40861 tggctttcat cgaaggaatt tgtggggaat tgatatcagt ggaaacagca acaaccgcag
    40921 gcagcggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat
    40981 ctgcagtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc
    41041 ccaccagcag accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca
    41101 aatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg
    41161 cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat
    41221 ctttacgccc tttggcgttg gtcagggctt taccgcccac acttaaggcc gtcacctgcg
    41281 cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttcaga tcgtattggc
    41341 ttattttggc atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat
    41401 cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca
    41461 tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcacgccc tttaatttgg
    41521 aaataaaact ttaatcacca atattgaaaa tgtcacacgc agataaatcc aactttcaat
    41581 attgttaagt tcct
//
LOCUS       DAZFAH010000021        39438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFAH010000021 DAZFAH010000000
VERSION     DAZFAH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.021"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..760)
                     /gene="aqpZ"
                     /locus_tag="ACHJWB_001043"
     CDS             complement(65..760)
                     /gene="aqpZ"
                     /locus_tag="ACHJWB_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4312718.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKYAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1187..2845
                     /gene="ybjD"
                     /locus_tag="ACHJWB_001044"
     CDS             1187..2845
                     /gene="ybjD"
                     /locus_tag="ACHJWB_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4312719.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTLRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2842..3798)
                     /gene="ybjX"
                     /locus_tag="ACHJWB_001045"
     CDS             complement(2842..3798)
                     /gene="ybjX"
                     /locus_tag="ACHJWB_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4312720.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3949..5064
                     /gene="macA"
                     /locus_tag="ACHJWB_001046"
     CDS             3949..5064
                     /gene="macA"
                     /locus_tag="ACHJWB_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4312721.1"
                     /translation="MKKRKTVKKRYVIALVIVIAVLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5061..7007
                     /gene="macB"
                     /locus_tag="ACHJWB_001047"
     CDS             5061..7007
                     /gene="macB"
                     /locus_tag="ACHJWB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4312722.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAS
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7080..7304)
                     /gene="cspD"
                     /locus_tag="ACHJWB_001048"
     CDS             complement(7080..7304)
                     /gene="cspD"
                     /locus_tag="ACHJWB_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4312723.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7454..7528
                     /gene="yljB"
                     /locus_tag="ACHJWB_001049"
     CDS             7454..7528
                     /gene="yljB"
                     /locus_tag="ACHJWB_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4312724.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7627..7947
                     /gene="clpS"
                     /locus_tag="ACHJWB_001050"
     CDS             7627..7947
                     /gene="clpS"
                     /locus_tag="ACHJWB_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4312725.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7978..10254
                     /gene="clpA"
                     /locus_tag="ACHJWB_001051"
     CDS             7978..10254
                     /gene="clpA"
                     /locus_tag="ACHJWB_001051"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4312726.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10658..10745)
                     /locus_tag="ACHJWB_001052"
     tRNA            complement(10658..10745)
                     /locus_tag="ACHJWB_001052"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10709..10711),aa:Ser,seq:gga)
     gene            complement(10999..11217)
                     /gene="infA"
                     /locus_tag="ACHJWB_001053"
     CDS             complement(10999..11217)
                     /gene="infA"
                     /locus_tag="ACHJWB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4312727.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11502..12206)
                     /gene="aat"
                     /locus_tag="ACHJWB_001054"
     CDS             complement(11502..12206)
                     /gene="aat"
                     /locus_tag="ACHJWB_001054"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4312728.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12248..13969)
                     /gene="cydC"
                     /locus_tag="ACHJWB_001055"
     CDS             complement(12248..13969)
                     /gene="cydC"
                     /locus_tag="ACHJWB_001055"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4312729.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13970..15736)
                     /gene="cydD"
                     /locus_tag="ACHJWB_001056"
     CDS             complement(13970..15736)
                     /gene="cydD"
                     /locus_tag="ACHJWB_001056"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4312730.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15859..16824)
                     /gene="trxB"
                     /locus_tag="ACHJWB_001057"
     CDS             complement(15859..16824)
                     /gene="trxB"
                     /locus_tag="ACHJWB_001057"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4312731.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17369..17863
                     /gene="lrp"
                     /locus_tag="ACHJWB_001058"
     CDS             17369..17863
                     /gene="lrp"
                     /locus_tag="ACHJWB_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4312732.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17998..22143
                     /gene="ftsK"
                     /locus_tag="ACHJWB_001059"
     CDS             17998..22143
                     /gene="ftsK"
                     /locus_tag="ACHJWB_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4312733.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQY
                     QQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMALLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            22298..22909
                     /gene="lolA"
                     /locus_tag="ACHJWB_001060"
     CDS             22298..22909
                     /gene="lolA"
                     /locus_tag="ACHJWB_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4312734.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22920..24263
                     /gene="rarA"
                     /locus_tag="ACHJWB_001061"
                     /gene_synonym="mgsA"
     CDS             22920..24263
                     /gene="rarA"
                     /locus_tag="ACHJWB_001061"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4312735.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24354..25646
                     /gene="serS"
                     /locus_tag="ACHJWB_001062"
     CDS             24354..25646
                     /gene="serS"
                     /locus_tag="ACHJWB_001062"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4312736.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25885..28329
                     /gene="dmsA"
                     /locus_tag="ACHJWB_001063"
     CDS             25885..28329
                     /gene="dmsA"
                     /locus_tag="ACHJWB_001063"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4312737.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28340..28957
                     /gene="dmsB"
                     /locus_tag="ACHJWB_001064"
     CDS             28340..28957
                     /gene="dmsB"
                     /locus_tag="ACHJWB_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4312738.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28959..29822
                     /gene="dmsC"
                     /locus_tag="ACHJWB_001065"
     CDS             28959..29822
                     /gene="dmsC"
                     /locus_tag="ACHJWB_001065"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4312739.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29857..30483)
                     /gene="ycaC"
                     /locus_tag="ACHJWB_001066"
     CDS             complement(29857..30483)
                     /gene="ycaC"
                     /locus_tag="ACHJWB_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4312740.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30797..31945
                     /gene="ycaD"
                     /locus_tag="ACHJWB_001067"
     CDS             30797..31945
                     /gene="ycaD"
                     /locus_tag="ACHJWB_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312741.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32155..33585
                     /gene="ycaM"
                     /locus_tag="ACHJWB_001068"
     CDS             32155..33585
                     /gene="ycaM"
                     /locus_tag="ACHJWB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4312742.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33586..34494)
                     /gene="ycaN"
                     /locus_tag="ACHJWB_001069"
     CDS             complement(33586..34494)
                     /gene="ycaN"
                     /locus_tag="ACHJWB_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4312743.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34594..35184
                     /gene="ycaK"
                     /locus_tag="ACHJWB_001070"
     CDS             34594..35184
                     /gene="ycaK"
                     /locus_tag="ACHJWB_001070"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO4312744.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(35266..36006)
                     /gene="pflA"
                     /locus_tag="ACHJWB_001071"
     CDS             complement(35266..36006)
                     /gene="pflA"
                     /locus_tag="ACHJWB_001071"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4312745.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36198..38480)
                     /gene="pflB"
                     /locus_tag="ACHJWB_001072"
     CDS             complement(36198..38480)
                     /gene="pflB"
                     /locus_tag="ACHJWB_001072"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4312746.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMSLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38535..39392)
                     /gene="focA"
                     /locus_tag="ACHJWB_001073"
     CDS             complement(38535..39392)
                     /gene="focA"
                     /locus_tag="ACHJWB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4312747.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFFHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9809 a         9624 c        10410 g         9595 t
ORIGIN      
        1 tgaatcaaca tataacaaca ttttaaaaaa aaagcctgac attgcgccag gccttctgcg
       61 ttaattaatt acgtttttcc agtagagtcc ggtaaatcag accaccgata ataccgccga
      121 caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc tggaagatag
      181 caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata ctgattaagt
      241 gaattagcgt taaggccaga ccaatggcaa ttggtgcaaa accagcagga gcgtatttgt
      301 cagttgcacc gtggatcact aacaggaagc ccgcactcaa taccagttca actaccagcg
      361 cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa gcaaaaccgc
      421 tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc gccgctgcaa
      481 caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc
      541 cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa atgtgaccaa
      601 cagcaaaggc catcgtcaga acggtcagac cgaacgccag cgccacgccg gcaaaaccga
      661 tgcctaattc cgggaagcct gcggccagaa cagcactacc acagccacca aaaacaagcc
      721 agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca attaaaaatt
      781 gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaaatgac gacggaaata
      841 atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa tccagtaaat
      901 ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga cagtgtagtg
      961 caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc taattatttg
     1021 attttttttg tttttgcaga cttatcagca agagggagta taacgcgatt attcgctcat
     1081 ttttcagaca tttgccatgc ttaaatgtga tgtcatcacg tattagcaag gcctttcccg
     1141 ttatactgcc agcgtaaagg ataagtcaca tatttctgga ggggatatga ttcttgagcg
     1201 cgttgaaatt gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa
     1261 cgtcctgatt ggggagaacg cgtggggtaa atccagcttg ctggacgcct taaccctgct
     1321 gctatcgcca gaatcagatc tctaccattt tgagcgcgac gatttctggt tcccgccggg
     1381 agatatcaac gggcgagaac atcatctgca tattattttg accttacgcg aatcgctgcc
     1441 aggccgacat cgggttcgcc gttatcggcc gctggaagcg tgctggacgc catgcaccga
     1501 tggctatcac cgtatttttt atcgtctgga aggggagagt gcggaagacg gcagcgtgat
     1561 gacactgcgc agttttctcg ataaagacgg acatccgatt gatgtcgagg atattaacga
     1621 tcaggcacgc catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat
     1681 gcgccgtatt cgtaacggca cggtgccaaa tgttcccaat gtggaagtca ccgcgcgcca
     1741 gctcgatttc ctcgcccgtg agttgtcctc acatccgcaa aatctctctg atggacagat
     1801 tcgtcaggga ctttccgcaa tggtgcagtt gcttgagcat tatttctctg agcagggggc
     1861 tggacaggcg cgatatcgtt taatgcggcg gcgggccagc aatgagcaac gaagctggcg
     1921 ctatctggac atcatcaacc ggatgattga ccgacctgga gggcgctcgt atcgggttat
     1981 tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc acattgagac tggataaaga
     2041 cgcgcgtccg ttattgctaa ttgaagatcc agaaacccgc ctgcatccga ttatgctttc
     2101 cgttgcctgg catctgttga atcttttgcc tttacagcgt attgctacca ccaactctgg
     2161 cgagctgctt tcgttaactc cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg
     2221 tgttgccgcc tggcgtctgg ggccaagtgg cttgagcacc gaagacagcc gccgcatctc
     2281 ttttcacatt cgttttaatc gtccgtcatc gctgtttgct cgctgctggt tgctggtgga
     2341 aggggaaacg gaaacctggg ttatcaatga actggcgcgc cagtgtggac accatttcga
     2401 tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt
     2461 tgcccgccga atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa
     2521 atatgccgct acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt
     2581 aacggcgtta ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt
     2641 gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg aatctgcgca aaattatctc
     2701 gaaagcgatc catcgctctt ccaaacccga tcttgccatt gaagtggcaa tggaggccgg
     2761 acgtcggggt gtggactccg taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg
     2821 gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc
     2881 gtcgagcatc tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat
     2941 ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc
     3001 aaccgattcc cagaaagcgt tgtaatcggc atggatcttg ccttctttat cacgataacg
     3061 caggctgcgg taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg
     3121 ttgggcaaac agacaggccg cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc
     3181 tttcgtcgca ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc
     3241 aataaacatc gttcttttcc cctgatattc acacagggta aacgtgatct ctgccagagg
     3301 aataccttcg ctgttgcgaa acaggattgt gctgtcacct tctttatcca ttgagatcat
     3361 catggtcagc tcaagcgtga actgctcgcc gtttttgcct tccagcttcg ccagttgcag
     3421 cccgggggta ttcaaatata agctgaattc ttccgccgac atacatccgc ggagtaacgc
     3481 ataatggtaa cgtaacgcct ccagcaattg cttacggcta agattcgccg caaggtaagg
     3541 gcgatgcaga cgcacaggca gtcgcggttg gcgcgttaac aacacattga gattaggcca
     3601 gtgggaaagt tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa
     3661 aaatttctgg cgaaaactac ggcgatgcca gaatttaccc ggccgacact gtccacgtgc
     3721 cagactaaga aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt
     3781 tagctgcgac atattcatga aatcaatggt tatacatgac gtcgatttca ccattgcgta
     3841 tcttaaccaa acatcaatag tgtgattact aacgtaaatt ttatggtttt gttgatattt
     3901 cgttgaagtt aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg
     3961 aaaaccgtga agaagcgtta cgttattgcg ctggtgatag tcatcgccgt actgattacg
     4021 ttatggagaa ttcttaacgc acccgtgccg acttatcaga cactgattgt gcgccccggt
     4081 gatttacagc aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg
     4141 ggcgcgcagg tcagcggtca gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa
     4201 aaagaccagc ttttaggggt cattgatcct gaacaggctg aaaaccagat caaggaggtc
     4261 gaagcaacgc tgatggagct acgcgcgcag cggcagcagg cggaagcgga gctgaaactg
     4321 gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag
     4381 gatctcgaca ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac
     4441 gcgcaaatca agcgcaatca ggcttctctc gatacggcta aaaccaatct cgattacact
     4501 cgcatcgttg ccccgatggc cggggaagtc acgcaaatca ccactctgca aggccagacg
     4561 gtgattgccg cacaacaagc accgaacatt ctgacgctgg cagatatgag caccatgctg
     4621 gtaaaagcgc aggtttctga agcggatgta atccacctga agccggggca aaaagcctgg
     4681 tttacggtac ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtactaccg
     4741 acgccggaaa aggttaacga cgctattttc tattacgccc gttttgaagt ccccaacccc
     4801 aatggtttgc tgcggctgga tatgactgcg caagtgcata ttcagctcac cgatgtgaaa
     4861 aatgtgctga cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa
     4921 gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac
     4981 gataccgatg ttgagattgt caaagggctg gaagcgggcg atgaagtggt gattggtgag
     5041 gccaaaccag gagctgcaca atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct
     5101 atcctgccgg tgatgagcag gttgaggtgc tgaagggcat caccctcgat atttatgccg
     5161 gtgagatggt agcgattgtt ggcgcttcag gttccggtaa atcgaccctg atgaatattc
     5221 tcggctgtct ggataaggcc accagcggca cctatcgcgt cgccggtcag gatgttgcca
     5281 cactggacgc cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt attttccagc
     5341 gttaccattt actttcgcat ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg
     5401 ccggtcttga gcggaaacag cggctgcttc gcgcccagga gttactgcaa cgactggggc
     5461 tggaagaccg tacagagtat tatccggcac agctttcggg tggtcagcaa cagcgcgtca
     5521 gcatcgcgcg ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccaaccggcg
     5581 cactggacag ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg
     5641 ggcatacggt gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga
     5701 tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg
     5761 ctggcgggac ggagcctgtt gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt
     5821 ttaacgaggc gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac
     5881 tgaccatgct ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg
     5941 acgccgccaa acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg
     6001 tctatcccgg gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg
     6061 acgacttaat cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc
     6121 aaaacctgcg cctgcgttat aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg
     6181 gcgattattt taatgtctat ggcatgacct tcagtgaagg aaacaccttt aatcaggagc
     6241 agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc
     6301 cccataaagc agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca
     6361 ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc
     6421 taccttacag cacgatgtcc gggcgagtta tgggccagtc gtggcttaac tccattactg
     6481 tcagggtgaa agaaggattt gacagcgccg aggcggaaca gcaactcacg cgtttacttt
     6541 cactgcgcca cggtaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaactg
     6601 ttgaaaagac cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg
     6661 tggtgggtgg tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg
     6721 aaattggcat tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga
     6781 tcgaagccgt actggtttgc ctggtcggtg gcgcgttggg aataacactg tcactgttaa
     6841 ttgctttcac cttgcagctt ttcttacccg gctgggagat tggtttttca ccgttggcac
     6901 tgctgctggc gtttctctgc tcgacggcca gcgggatttt atttggatgg ttacccgcac
     6961 gaaatgcggc acgactggat ccagtagatg ctctggcacg agagtaattt ttgagataaa
     7021 aatgccagcc gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc
     7081 tatgcgactg ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc
     7141 ccctggtgga catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc
     7201 tgaatggtgg aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca
     7261 aaccctttgg cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg
     7321 caaatcttat acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct
     7381 cgccaactct agaaatacaa tttagagaat tagggcgagc cgtcaagcgt ttgacagggg
     7441 ccagggggca ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac
     7501 agatgtcgct gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag
     7561 gcaaataagc tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa
     7621 ctgacaatgg gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt
     7681 cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact
     7741 ccgatggagt ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca
     7801 acgcaattga tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc
     7861 gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca
     7921 ttgctgtgta cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg
     7981 ctcaatcaag aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt
     8041 catgagttta tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg
     8101 gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt
     8161 attgaacaaa ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg
     8221 ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat
     8281 gaggttaccg gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca
     8341 tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg tgaattttat ctctcatggc
     8401 acgcgtaaag acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa
     8461 caagctggtg gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcgc
     8521 gtgggcggaa tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt
     8581 ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc
     8641 gcgattgcag aaggtcttgc ctggcgaatt gttcagggcg atgtgccgga agtgatggct
     8701 gactgtacga tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc
     8761 gactttgaaa aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc
     8821 ctgtttattg atgagatcca caccattatc ggtgcggggg cagcgtctgg tggtcaggtc
     8881 gatgcggcta acctaatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg
     8941 acaacctatc aggagttcag caacattttc gagaaagacc gtgctctggc acgtcgcttc
     9001 cagaaaattg atattactga accgtcaatc gaagaaactg ttcaaatcat caatggcctg
     9061 aaaccgaagt atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg
     9121 gtagagctgg cggtgaaata catcaatgat cgtcatctgc cggataaagc gattgatgtt
     9181 atcgacgaag cgggcgctcg cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt
     9241 aatgtggcgg atattgagtc cgtggtggcc cgtattgcac gcattccaga gaagagtgtt
     9301 tctcagagtg accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc
     9361 ggtcaggata aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta
     9421 ggtcacgaac ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa
     9481 acagaggtga cggtacagct ttcgaaagct ttgggcattg agcttctgcg ctttgatatg
     9541 tccgagtata tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt
     9601 ggttttgatc agggcggttt gctgactgat gcggtcatca agcatccaca tgcggttctg
     9661 ctgctggacg aaatcgagaa agcgcaccct gacgtgttca atattctgtt gcaggtgatg
     9721 gacaacggta cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg
     9781 gtgatgacca ctaacgccgg ggtacgggaa actgagcgca aatccattgg tcttatccac
     9841 caggataaca gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt
     9901 aaccgtctcg acaacattat ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg
     9961 gtggataaat tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa
    10021 gtgagccagg aagcgcgtaa ctggctggcc gaaaaaggtt acgaccgggc aatgggcgca
    10081 cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg
    10141 tttggttcgc tggtggacgg cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag
    10201 ttgacttacg gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat
    10261 tgttaggtag gttggtcaag tccgtaatct cgaaagaggt tacggacttt ttgtttatgg
    10321 ggtggaggag gttcagaccc tttttttaat gatgatggta agttgttgat aattagtgct
    10381 gcgggaaggt aaggataaaa aagggtgctg caggagaatg ggatggtttt gctttattaa
    10441 caacgggcta aacgtgtagt atttgagttc actgccgtac aggcagctta gaaatcgacg
    10501 gggtgcggta aaacctttgc gaacgcgttc actgccgtac aggcagctta gaaattcaca
    10561 ggtaacatac tccacccgcc caccatgttc actgccgtac agacagataa aatgcgaaaa
    10621 aaaagctcgc actttcgtac gagctcttct ttaaatatgg cggtgagggg gggattcgaa
    10681 cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc
    10741 tcaccaaatt gttttgctac caaacctcat gggtggcaac ggggcgctac tatagggagt
    10801 tggagtaaaa cggtcaagaa gaattttaat gataattatt gtttgctcat actgtaaaca
    10861 agttgtgcag tatatctaca tcgagacaag ttacggactt atacttccaa agtacttcat
    10921 acatatcaca aaataaaaag gccggttaaa ccgacctttt actcgttctt tctcttcgcc
    10981 catcaggcgg taaaacaatc agcgactacg gaagacaatg cggcctttgc tcaggtcgta
    11041 cggggtcagt tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat
    11101 tttaccggag atgtgtgcag taaccacgtg accgttttct aactctacgc ggaacatggt
    11161 attaggcaac gtttcaagaa cggtaccttg catttcaata ttgtcttctt tggccatcta
    11221 atcctctggg gtatcactac cgtaatttga accggcaaga taatgccgaa gttctgtaaa
    11281 taagtaaaga tttgcgcgct aaatcgcaac aaacaggttc ggcacattac tccgaaaaca
    11341 cacggctaag ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa acgatgggcg
    11401 ttacctgacg cgaaaaattc cttatcggca gcggggtaat gagcgtaacc aactctgcga
    11461 ccgcaattat aacactctgg ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac
    11521 aagcatcgtg gtacccagaa attattcggc aatcgtccga ggcgcatttg attgagataa
    11581 ttaaggtaat cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg
    11641 acctggcagt cgataagctt accgccatga ccgataaatt cctcacagaa taccagaagc
    11701 gccgttttag acgcattttc catccggctg aacatggact cgccacaaaa tagcgttccc
    11761 tgggccacgc cgtacatacc gccgacaagc tcatcttcac gccagacttc aatggagtgg
    11821 gcatggccga gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt gatccaggtt
    11881 ccttcttcgc gatcgctggc acagccttca atgacctgac caaaagcgta attcatcgtg
    11941 acacgatagg gcgagcgttt atgaaatcgc ttcatactac ggctgatatg cagtgattct
    12001 ggccatagca ccgcgcgggg atcgggcgac caccagagga tggggtcgcc tggagaaaac
    12061 cacggaaaaa taccacgctg gtaagccatt aacaggcgcg cagggctaag atcgccccca
    12121 agtgccagca ggccgttagg ctcacgtaat gcgccttccg gggaagggaa ggctattgaa
    12181 tggcgagaaa gctgaaccag gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa
    12241 taatagctta caaaccctgc ttgaactggt aataacgccc ctgtctggca agcagctctg
    12301 cgtgagtacc ttgctcaata atttgcccgt tgtccatcac tattatttgt tggaaacgag
    12361 agagtccgcg aagtcgatgg gtaaccatta acaccgtttt ctcacgcatc atttctgcaa
    12421 gcaattcaag gatctggctt tcggttgtgg catctaagcc ttcggtaggt tcatccagca
    12481 acaccagtgg cgcatcatgt aacagcgcac gggcgatagc cagacggcgc agttcaccac
    12541 cggagagctg gcgtccgcct tcacctaacc aactgttgag acctgcatcc tcgagcagct
    12601 tctccagacc aacgcgacgc aagctctccg ccagagcctc atcactactg ccaggcgagg
    12661 cgagtaacag attatcacgc agcgtggcgc taaacagatg cactcgctga ggaacaacgc
    12721 tgatggtctg tcgtagagcc gcttcattca ggctggctat ggggctatcg ttaagcaaaa
    12781 tctctccctg ttgcgggtcc caagcgcggg tcagcagttg taacagtgtt gatttgccgc
    12841 atccggttcg cccgagaatc gcaatatgtt ccccggcgtt tacctgaaga gaaatccctt
    12901 taagtgcctg ttgggattgc tccggataag tgaactgaac atcccgtaat gtcagcgaaa
    12961 cgcgatcggc aacacgagtt tgggtatcag ggaaggtgac ttccggtttt tgatccgtta
    13021 aatcagtgat ccgaacggca gaggcaatga cttgccccag atgctgaaat gcacccgtta
    13081 ctggtgccag tgcttcaaac gcggctaacg cgcagaagac aaacagggca attaacgcgc
    13141 cgggttgagc attgccgcca acgccgccag acgccatcca cagcatcagg atcaccgcta
    13201 acgcgccaat gagcagcatt atcgcttgcg acaatgcggt cagttcagat tgacggcgtt
    13261 gcgcttccag ccattgaatt tctgtattct ctagttgcgt gcgataacga tcgctggcac
    13321 caaaaatggt cagctcagct tgcccttgca gccaggccgt cagttgttgg cgatactgtc
    13381 cgcgaagatg agtcagattt tgcccggtgc tttttcccgc acgataaaac agcggtggca
    13441 tcaggaaaag cgtcagtaac ataatgccgc ccagcgtaaa ggcgagggtg aaatcaagga
    13501 aacttaaccc gattgtcacc accataatca ccacaaaagc gcccaccagc ggtgagataa
    13561 cgcgcagata aagatgatcg agcgtatcaa catccgccac cacgcgattg agcaattcgc
    13621 cctgacgata gcgcgccagt ccggcagggg agaggggcag caatttgctg aaggtgtaaa
    13681 tgcgcagatg ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt tcaaaataac
    13741 gcccggcagt acgggtgatt gctgcgccac gcacgcccgc agcgggtagc atatagttga
    13801 agctgtacag tccggcaacc cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg
    13861 tcaacagacc gatactggcg agcagcgtga caattgccag cacgatacca agacttaaca
    13921 tccatttatg acgtttatac agtgccagat agggtagcaa agcgcgcatt taaatctcct
    13981 cctgacgatg ggccagtaat gtggcgaatg ggccaccagc cacacttaat tccgcgtaac
    14041 gtccttgctc aataatccgg ccatcctgca taacccaaat gacatcccag tcagcaagat
    14101 cttctaactg gtgggtgacc attaacgttg tctggcgcag agaggcggca ttcagcgcct
    14161 ccattacgcg ctgttcactg tgagcatcaa ggctggcagc gggttcatcc aacagtaata
    14221 gcgaacaggg atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa
    14281 ggcgggcagc ctggtcgcca acaggtgtat caacgccttg tggcaggagc ggtagaaact
    14341 cgctgaccca ggcgttatcc agcgctgctt gtaattcttg ttcgctggca tcaggtcgcg
    14401 ccagtagtac gttatcccgc aatgttgctg ccggtaattg tgggttttgc ccaacccagg
    14461 agagatgttt acgccatgat tctggtgata aatcgcgtaa ttctatcccg ttgattcgta
    14521 atgatccctg atatgagaga aaaccagaaa gcgcgttcag cagtgagctt ttacctgaac
    14581 cgctgcgacc aaccaacact gcacgttggc ctgctggcaa agtaaagttc agcggtccgg
    14641 ccagcgtttt accttccggc gacgtgataa acagatcctc ggcctcaatg gtcaccggat
    14701 cggtcgatgc taattccacc tcgccacgct gcggatgggc gagcggggtt tccataaacg
    14761 ttttcagact gtcagctgca ccaacagcct gggctttagc atgataaaac gtaccgagat
    14821 cgcgtaatgg ctggaaaaac tctggcgcaa ggatcagggc cagaaaaccc gcagccagcg
    14881 tcacgccagt atcgtagtga ccaaaatcca gctcgccgag ataggaaaaa ccaaagtaga
    14941 ccgccaccag agcaattgac agcgaggtaa aaaattcgag aatgccggag gataaaaatg
    15001 ccagccgtag cacttccatt gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt
    15061 caatttcagc ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga
    15121 ggaaatgccc acttaagcga gcaagagcga gaaagttacg tcggttagca tcggcagccc
    15181 ccattccaac cagcgccata aacaacggaa ttaacggtgc agtgcccagc agaatgagcg
    15241 ccgcagccca gtttgagggg aagattgcca ccacaatcag caacggcacc gacactgcca
    15301 gcgccatttg cggcaggtag cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta
    15361 ccagcgtcgc ccagctcccc gcaggtttac cctgaatcca cgctggccct gcttgttgca
    15421 gacggtcgag aacctgccgg cggatggcaa agcggatatg ctgcccggcg tgataaccca
    15481 cccgttcgcg taaccagacc acccatgcgc gcagtacaaa ggtcagaacc agtaacgtaa
    15541 agggaagcag cagggcttca cggggaatat tctccataat catatgttgc agaattcgcg
    15601 ccatgaacca ggcctgggca atgatcaata tgccgctcac aaagcccagc agacgagaaa
    15661 tattcagcca acgttgggag atgacgcttt gctgttttaa ccagcgggtt aactcttttt
    15721 gacgagattt attcattgca cgcttagcag gtgagttatc agaattattt gcagagcaat
    15781 gttacaacgg ggaaaaaata aaggcgaccc atagtcgcat ggtgtcgcct tctttacttt
    15841 tgttactgat ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc gttccgcatc
    15901 aagtgctgcc atgcagcctg taccggccga agtaatggcc tgacgataaa tatgatccat
    15961 cacgtcaccc gcggcaaaga cgccagggat gctggtctgg gtggcattac catgaatacc
    16021 cgactgtact ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa tggcagtatt
    16081 cgggctgtga ccgatagcaa caaacagacc ggcaacgtcg agtgactcga tgttatcgct
    16141 gttttgcgta tcgcgcagac gaacgccagt gacacccatt tgatcgccgg tcacttcttc
    16201 cagcgtacgg ttggtgtgca gaatgatgtt gccgttctcc actttatcca tcagacgctt
    16261 aataaggatt ttttccgcgc ggaaaccgtc acggcggtga atcagatgca cttccgaagc
    16321 gatgttagac agatacagcg cctcttcaac cgcggtattg ccgccgccga tgaccgcaac
    16381 tttctggttg cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt
    16441 aaaggcttct tcagagggca ggccgagata gcgtgcagaa gctccggtgg caataatcag
    16501 cgcgtcgcaa gtgtattcgc cgttatcgcc attcagacgg aacggacggt tttgcagatc
    16561 caccttgttg atatgatcaa aaatgatctc agtttcaaac ttggtggcat gttcgtgcat
    16621 gcgctccatt aataacggac cggtcagatc gtttggatcg ccaggccagt tttccacttc
    16681 cgtggtggtg gtcagttggc cgcctttttc catgccggta atcagcacag gttgcaggtt
    16741 ggcgcgcgcc gcgtagacag cagcggtgta tcccgccggg cctgaaccca ggataagcag
    16801 tttactgtgt ttggtcgtgc ccatgagatc cccatagttg ttggcagaca atgggcagga
    16861 ttgtagggaa tttacagacg taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa
    16921 tcggcagcat cgataagcag gtcaaattct cccgtcatta tcacctctgc tacttaaatt
    16981 tcccgcttta taagccgatt aaatgatgaa taaacgcccc tgttaatgaa tatctggcat
    17041 gttgtactaa aaatcgatgt tttgctttga caatcccctg gtgttttgcg aaaacattcg
    17101 aggaagaaaa aaaacagtat tcttatatgc gcataaccat gcatgtaaat accatgttta
    17161 ccgtgctagt gaaatctacg tatggcgtgg acagacgcta ttcgtgatgt cgatagctgc
    17221 cacaaggcaa cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat
    17281 ttcggtctat cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt
    17341 agggaaggaa tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga
    17401 tctcgaccgt atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa
    17461 cgtcgagctt tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg
    17521 gctggaaaga caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga
    17581 tgcatcactt ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga
    17641 acaattcaat accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg
    17701 tgatttcgac tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct
    17761 gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga
    17821 agaagtcaag cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa
    17881 atcggcgtat tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg
    17941 gtactgttgt ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg
    18001 agccaggaat acactgaaga caaagaagtc acattgacaa agttaagtag cggccgtcgc
    18061 cttctggaag cgttgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta
    18121 ctaagcttta acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat
    18181 aatttaggtg ggatgcccgg tgcgtggctg gcagatacgc tgttctttat ttttggcgtg
    18241 atggcttaca ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag
    18301 tccagtgacg aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg
    18361 ctcatcctta cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc
    18421 tccggtggcg tcattggcag cttactaagc actacgctac aaccactgct acacagtagc
    18481 gggggaacta ttgcgctgct ctgcgtttgg gctgcgggcc tgacgctgtt caccggttgg
    18541 tcatgggtga ccattgctga aaaactcggc ggctggattt taaacattct caccttcgct
    18601 agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag atgaatatga agatgacgaa
    18661 gagtatgaag atgaaaacca cggcaaacag catgaatcac gccgtgcccg tattcttcgc
    18721 ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa
    18781 acagacgctg cgttgttctc cggtaagcgg atggatgatg acgaagagat tacctacact
    18841 gcacgcggtg tggctgccga ccctgacgac gtcctatttt cgggcaatcg tgcaacgcag
    18901 ccagaatatg acgaatacga tccattatta aacggtgcgc caattaccga acctgtcgct
    18961 gtagcagctg ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact
    19021 cagacgccgc ctgttgcctc tgttgatgtt ccaccttcgc aacctacagt agcctggcag
    19081 cctgtaccgg gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca
    19141 cagcagccac aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta
    19201 cagccacagc agccgtatta tgcacctgca gctgaacaac ctgcgcaaca accttactac
    19261 gctcctgcgg cggaacaacc tgttcagcaa ccgtattatg caactgcgcc ggaacagcct
    19321 gcgcaacagc cgtattatgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa
    19381 gccgaagagc agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat
    19441 cagcagccag ccgctcagga gccgttgtac caacagccgc aacccgttga acagcagcct
    19501 gttgtggagc ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactat
    19561 tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa
    19621 ccgattccag aaccggttaa agaaccagaa ccgatcaaat cttcgttgaa agcgccttct
    19681 gttgcagcag tacctccagt agaagccgct gccgctgttt ctccgctggc atctggcgtg
    19741 aaaaaagcga cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg
    19801 gcaaatagcg gtggaccgcg tccgcaagtc aaagagggga ttggcccgca gttgccacga
    19861 ccgaaacgta tccgcgtgcc aactcgtcgt gaactggcgt cttacggtat taagctgccc
    19921 tcacagcgtg cggcggaaga gaaagcccgt gaagcccagc gcaatcagta tgattctggc
    19981 gatcagtata acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc
    20041 gcccagacac agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca
    20101 gaagatgcag atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa
    20161 caacgttatt ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa
    20221 ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt
    20281 gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc agcagcagta tcagcagcca
    20341 caacagccgg ttgcgccaca gcagcaatat cagcagccgc aacagccggt tgcgccgcag
    20401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    20461 caaccggttg cgccacagcc gcaatatcag cagccgcaac aaccagttgc gccgcagcag
    20521 cagtatcagc agccgcaaca accggttgca ccgcagccgc agtatcaaca gccgcaacaa
    20581 ccggttgcac cgcagcagca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    20641 agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    20701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctct cctggtggaa
    20761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtca attactctcc ggggccggtt
    20821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    20881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    20941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    21001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    21061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    21121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    21181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    21241 tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat gaaagatgcc
    21301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact gatgtctgcg
    21361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    21421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    21481 ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct gatgatgacg
    21541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    21601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    21661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    21721 cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta ctctgggccg
    21781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    21841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacttccgac
    21901 agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga tccgttgttc
    21961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    22021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    22081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    22141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    22201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    22261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg
    22321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    22381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    22441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    22501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    22561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    22621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    22681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    22741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    22801 tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    22861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    22921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    22981 ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg gggaagccgt
    23041 tgccgcgcgc tatcgaagcc gggcatttac attctatgat cctctggggg ccaccgggta
    23101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    23161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    23221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga ttcaacaaaa
    23281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    23341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtctatc
    23401 tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    23461 agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga cgcgccattg
    23521 ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    23581 tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg accgaaatcg
    23641 ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat ctcatttccg
    23701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg tatgcgcgaa
    23761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca attgcttccg
    23821 aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    23881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    23941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg gcggatgctc
    24001 gcgaacgtcc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    24061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    24121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    24181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    24241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    24301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    24361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    24421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    24481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    24541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    24601 atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    24661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    24721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    24781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    24841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    24901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    24961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    25021 tggaagaaga agcagatacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    25081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    25141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    25201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    25261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    25321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    25381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    25441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    25501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    25561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    25621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgcctgcg
    25681 ggcgcttttt ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata
    25741 ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag
    25801 cctatatagc gagctgctat agaaataatt acacaatacg gtttgttact ggaatcaatc
    25861 gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct
    25921 gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc
    25981 agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca
    26041 aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg
    26101 ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat
    26161 gacaattacg acggcctgca ccaggttcgc gcctgtctgc gtggacgttc catgcgtcgc
    26221 cgtgtctata atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa
    26281 ggcaaatttg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag
    26341 cgcctgatca aagagtacgg caacgaatcc atctatctga actatggcac cggtacgctg
    26401 ggcggcacca tgacccgctc ctggcccccg ggaaataccc tggtcgcgcg cctgatgaac
    26461 tgctgcggcg gttatctgaa ccattacggt gactactctt ccgcgcaaat tgcggaaggt
    26521 ttgaactata cctacggcgg ctgggcagat ggtaacagcc cgtcggatat cgaaaacagt
    26581 aaactggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg cggtggggtg
    26641 acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat catcgatccg
    26701 cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccgggaaca
    26761 gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct ggtggatcag
    26821 gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc cagtgcgccg
    26881 aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaaca
    26941 ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa actggctcgt
    27001 gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct
    27061 aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt
    27121 attaacggag gcaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg
    27181 ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt
    27241 gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga taagctggat
    27301 gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa
    27361 atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat tgtggttatc
    27421 gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct
    27481 tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta cgtgattttc
    27541 aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa
    27601 ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca ggaagagtgg
    27661 atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag
    27721 ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa
    27781 gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa aattgagatt
    27841 tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc
    27901 gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgtt gaacaaacag
    27961 tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac ttacggcaac
    28021 gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct tgatgcgcaa
    28081 aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat
    28141 attgaggcga aagtgacgcc acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc
    28201 tggtatgacc cggatacaaa acgtgtcgat aagggcggtt gtatcaacgt actgaccact
    28261 caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa
    28321 aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg
    28381 caccggttgc aaaacctgcg agctggcctg taaagactac aaagatttga cgccagaagt
    28441 cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg
    28501 gcaccagaac gtgtttgcct actatctgtc gatttcatgt aaccactgcg aagatccggc
    28561 ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt
    28621 cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt atggcgcacc
    28681 gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc
    28741 ggaggggaaa aagccgatct gtgttgaatc ttgcccgctg cgggcgctgg atttcggccc
    28801 tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc
    28861 tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga
    28921 taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat
    28981 ggccgctgat gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg
    29041 ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca
    29101 tgtttggttt atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt
    29161 caccaatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa
    29221 tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga
    29281 aaaagctgtc accggcattg cgtacgctgt ggctgatagt gacgatggtt cttggcgtca
    29341 tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca
    29401 gcatctggac gccgatgggc ttcttcctga cgatgtttat gggcggcccg ttactgggtt
    29461 acctgttgtt gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagcaattt
    29521 ctgtactggc actggtagtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga
    29581 ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt
    29641 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
    29701 gttatcagcc tgcggttccg ctactttctg tatcattcat tctgcttctg gcaggggaat
    29761 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
    29821 aatcataaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
    29881 ataactggtc atcaaattac gataatcagg aatgtggtta gagaacaacg tcgccagtcc
    29941 ttcaatatca ttacgccagt cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa
    30001 ttgcgcacca gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt
    30061 ccctgacgcg tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg
    30121 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
    30181 tttgacagct tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggcgagcaat
    30241 gtaaggtgta tcggggaatt gtgctttcag ttctggaact aatgggccgt taggaccggt
    30301 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc
    30361 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
    30421 ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt
    30481 catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcgatcagac gcgatgcatt
    30541 gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa
    30601 cgaagcgtat gaaatttgtg ttagttcaat aaaaacaatc agatgagact aatcatctct
    30661 tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg ggatatcgcg
    30721 tattttcacc cattgacaat gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc
    30781 ctcacggttt taattcatgt ccacgtatac ccggcctgtc atgcttttgc tgtctggcct
    30841 gcttttgttg actctggcga ttgcggtgtt aaatacactc gtgccgcttt ggctcgccca
    30901 ggaacacatg tccacatggc aggtaggcgt tgtcagctca tcctatttta ccggcaacct
    30961 tgtcggtaca ttgctgacag ggtatgtcat taagcgcatt ggctttaacc gcagctatta
    31021 tctggcctcc ttcatttttg ccgctggctg tgccggcctt ggcctgatga ttggattctg
    31081 gagctggttg gcttggcgtt ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt
    31141 tgagagcgcg ctgatgtgca gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta
    31201 tatgatggtt tattacgtgg gaacgttttt aggccagtta ctggtcagca aagtttcaac
    31261 cgagctgatg tccgtattgc cgtgggttac aggtttgacg ttggcaggga tcttaccgct
    31321 gttgtttacg cgtgtgctga atcagcaggc tgaaaaccat gattcgacgt caattacgtc
    31381 aatgctaaaa ctccgtcagg cgcggcttgg cgtgaatggc tgcattatct caggaatcgt
    31441 tctgggatct ctatatggcc tgatgccgct gtacctcaat cacaaagggg tgagcaatgc
    31501 cagcattggt ttctggatgg cggtactggt cagtgcgggt atccttggac aatggccgat
    31561 tggacgtctg gcggataagt ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt
    31621 cattctcggc agtatcgcga tgcttagcca ggcggcgatg gccccagcgt tattcatcct
    31681 cggtgccgct ggctttacgc tatatccggt ggcgatggca tgggcttgcg agaaagttga
    31741 acatcatcaa ctggtggcga tgaaccaggc cttactgttg agctatactg tgggaagtct
    31801 gcttggcccg tcatttaccg ctatgctaat gcagaatttc tccgataatt tattgtttat
    31861 catgatcgcc agcgtatcgt ttatctattt gctgatgctg ctgcgcaacg ccggtcatac
    31921 gccgaaaccc gttgctcacg tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac
    31981 actctgcttt tttgtttctt ctatctgact tgctttattc caaattttat tcgtttaaaa
    32041 ataaaatgtg cagcaggtta taattttgca tttcgctatt tccgcacttc ttatttgccg
    32101 cgcataatcc ctcgttttac agatgcccct ttaattttgg cgaaggattt gtctatggct
    32161 gggaatgttc aggaaaaaca gttgcgatgg tacaacattg cgctgatgtc ttttatcact
    32221 gtctggggtt ttggcaacgt tgttaataac tatgccaacc aggggctggt ggttgttttt
    32281 tcatgggtgt ttatctttgc actctatttc acaccttatg cgctaattgt tggtcagtta
    32341 ggctcgacct tcaaagatgg gaagggcggg gtcagtacct ggattaaaca cacgatggga
    32401 cccggactgg cttatctcgc cgcgtggacc tactgggtgg tgcatattcc ctatctggca
    32461 caaaaacccc aggcaattct gattgcgctc ggttgggcga tgaaaggcga cggttcgcta
    32521 atcaaagaat attcagtcgt agcgttacag gggttaacgc tggtgctgtt tatcttcttt
    32581 atgtgggttg cttcacgcgg tatgaaatcg ctgaaaatcg tcggttctgt ggcagggatt
    32641 gcgatgtttg ttatgtcgct cctgtatgtg gcgatggcgg taaccgcgcc tgcgattact
    32701 gaagtgcata ttgcgaccac aaacattacc tgggaaacgt tcattcctca tatcgacttt
    32761 acctacatta ccactatttc aatgctggtt ttcgcggttg gcggagcaga gaagatttct
    32821 ccttacgtta atcaaacgcg caacccagga aaagaatttc caaaagggat gttatgcctg
    32881 gcggtgatgg ttgcggtttg tgccattctg ggctcactgg cgatggggat gatgtttgac
    32941 tcgcgtaata tcccggatga cttaatgact aacggtcaat attacgcctt tcagaagctg
    33001 ggcgagtatt acaacatggg taatacttta atggtgattt acgccattgc gaataccctg
    33061 ggacaagtag ctgcgctggt attctcgatt gatgccccgc ttaaagtgct attaggtgat
    33121 gctgacagca aatatattcc agccagttta tgtcgtacca acgcttctgg tacgcccgtt
    33181 aatggctatt ttctgaccct ggtgctggtg gcaattctga ttatgctgcc gactctcggc
    33241 atcggtgata tgaacaatct ctataaatgg ctgttgaatc ttaactcggt agtgatgccg
    33301 ctgcgttatt tatgggtatt tgttgcattt attgcagtcg ttcgcctggc gcagaaatat
    33361 aaaccagaat atgtctttat tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt
    33421 tttgccttta ccgcctttgc ctgcttgacg gggatcttcc cgaaaatgga agccttcact
    33481 gcagagtgga ccttccagtt ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta
    33541 ggactgatat tcccgctgct ggcgcgtaaa gcgaatagta aataattatt gtggtcggtg
    33601 acgctgactc tggcagatct gacccgtttt tacataatcg agaaatgcgc gcaagccgca
    33661 ggacatatac tggcggtttg gataatagat ctggaagcct gaacgctctg tgctccagtc
    33721 ttccagtaca cattctaaac gaccagtatc aaggtactcc ttgatctgtt catagagcag
    33781 atacccgata ccagcaccca tcaaaacggc ctccagttca gcatcaacat catccagaat
    33841 gatatttcct gcgacagcaa tttccagctc ctttgcaaat tgccagtgga aaggtttgcc
    33901 gctgggataa cgaaaaacaa cacattgatg attaagcaga tcatgaggat ggcggggttt
    33961 gccatatctt gcaaagtact ctggcgttgc tgctacacat aatttcactg gtgggccgat
    34021 cgccacgctg atcatatctt tttcgacgat gccactaagt cgaacgccag cgtcaaagcc
    34081 ctgttggacg atatcgacaa gagaatcgtc agttgtgagc tcgactttaa tatcaggata
    34141 ttcacgtgtg aaaccaacca gtaaaggcat taaaaatata cgtgcagcta cccgggcggc
    34201 attgattttg agcgttcctg tcggtgtcag acgaaaatca ttcatctcat ctaacatgat
    34261 ctgaatttcg tcgaaagctg ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa
    34321 cgatacgctt cgggttgtcc gattgaataa gcgaatttta agacgttgtt ccagtgtttt
    34381 aatactatgg ctaatggcgg acgaggataa gcctaactca tcgcccgctg cacgaaagct
    34441 ttgattacgg gcgacggcaa agaaagtggc aaagtcagac atattcatcc gcattagtga
    34501 aaatccttca ttaactcatc ctgaatcatc acgtttacag gattatgccg cctgcgtaaa
    34561 gtgcgctcca gaacttaacg tggaggtaaa attatgcagt ctgaacgtat ttatttggta
    34621 tgggcccatc ctcgtcatga ttcattgacc gcaaatattg ctgatgcgat ccatcagcgg
    34681 gcaatggagc ggaaaataca ggtgacggaa ctcgatttat atcggcgtaa tttcaaccca
    34741 gtgatgacgc cggaagatga accagactgg aagaatatgg ataaacgtta ttctccagag
    34801 gttcatcagc tttattcaga gctgcttgaa catgacacgt tagtggtggt ttttcctctc
    34861 tggtggtaca gcttcccggc aatgctaaaa ggatatattg acagagtatg gaataatggg
    34921 ctggcttatg gagatgggca caaattacca ttcaataaag ttcgttgggt ggcgctggtt
    34981 ggaggagaca aagaatcatt tgtccagatg ggctgggaaa aaaatataag cgattatttg
    35041 aaaaatatgt gcagttatct tggtattgaa gatgccgatg tcactttctt gtgtaataca
    35101 gtggtattcg atgggcaaga acttcatgcg agctattatc agtcgttatt atctcaggta
    35161 cgggatatgg tagatgcact ataaaatgtg ttaaaaacgc tgtagcagaa tgaagcgcgg
    35221 aataaaaaag cggcaactca ataaagttgc cgctttacgg ggaaattaga acattacctt
    35281 atgaccgtac tgctcaagaa tgcctttcac gcgttccatg gtctctttct taggtggttt
    35341 aacaccgtcg agtttgtact cttcacccat tgccacccat ttgtgtttgc ccagctcgtg
    35401 gtaggggagg agttcgattt tctcaacgtt gcccatatca cgggtaaatt caccgaggcg
    35461 atgcgctgaa tcgtcatcgt cagaccagcc agggacaaca acgtagcgga tccacacctt
    35521 cacatttttg ttcgccagat atttggcgaa ctccagcgtg cggtggttgg aaactccaac
    35581 cagattttgg tggatctcgt cgttcatctg tttgagatcg agcattacca ggtcggttac
    35641 ttccagcagt tcatcaatca ccggatcgta acgacgaaca aaaccgttgg tgtccagaca
    35701 ggtatgaatg ccttcttttt tgcaggcgcg gaaccagtca cgaacaaact cagcttgtag
    35761 gattgcctca ccgccggatg cggtaacgcc gccgccggaa gcgttcataa agtggcgata
    35821 ggtcaccacc tctttcatta aatcttcaac ggtaacttct ttaccgccat gcgtatccca
    35881 ggtgtcgcgg ttatgacaat acaggcagcg catcaggcag ccctggaaaa aggtgataaa
    35941 gcgaatacct gggccgtcta cggttccaca ggattcaaag gagtgaatgc gaccaataac
    36001 tgacattgcg gtgtttctcc agatgtggcc catctgaggc cgtgttggtg cgcagctcga
    36061 aggctacgtc gagtctgttt tggcagtcac cttaaagtat agatagctga caaaaaaggc
    36121 tctcgtgcta aaaaaggccc cactttcgtg gagcctttat tgtacgcttt ttactgtacg
    36181 atttcagtca aatctaatta catagattga gtgaaggtac gagtaataac gtcctgctgc
    36241 tgttctttag tcagcgagtt gaaacgtact gcgtagccag atacacggat ggtcagctgc
    36301 ggatattttt ccgggttttc catcgcgtcg agcagcattt cacggttcat cacgttaacg
    36361 ttcaggtgct gaccaccttc gatggatgct tcgtggtgga agtaaccatc catcagacca
    36421 gccaggttgg tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac gatagagaag
    36481 gtgtaggaga taccatcttt agcgtaagca aacggcagtt tagcaacgga agtcagagac
    36541 gctacagcac ctttctggtc acgaccgtgc atcgggttag cacccggtcc gaacggcgcg
    36601 ccagcacgac gaccgtctgg ggtgttacca gttttcttac catacacaac gttagaagtg
    36661 atggtcagaa cagactgagt cgggatagcg tcacggtagg tgtgcagttt ctgaattttc
    36721 ttcatgaaac gttctaccag gtcaacagcc aggtcatcta cacgcggatc attgttacca
    36781 aactgcgggt attcgccttc gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc
    36841 ggtttaactt tcgcatattt gattgcagac agggagtcag cagcaacgga cagaccagcg
    36901 ataccacacg ccatggtgcg gataacgtca cggtcgtgca gcgccatcag agaggcttcg
    36961 tagctgtact tgtcgtgcat gtagtggatg atgttcagtg cagtgatgta ctgtttagcc
    37021 agccagtcca tgaagtgatc catgcgctcc atcacttcat catagttcag gacatcgcct
    37081 ttgatcggtt cagacttcgg accaacctgc attttcagtt tttcgtcaac gccgccgttg
    37141 attgcgtaca gcatggtttt cgccaggttt gcacgcgcac cgaagaactg catttgttta
    37201 ccaacgatca tcgggcttac gcagcaagca atagcgtagt catcgttgtt gaagtccgga
    37261 cgcatcaggt catcgttctc atactgcaga gaagaggtgt cgatggacac tttagcggcg
    37321 aatttcttga agttcagcgg cagtttttca gaccacagaa tggtcatgtt cggttccgga
    37381 gacggaccca tggtgtacag ggtgttcagg aaacggaagc tgtttttggt aaccagggta
    37441 cgaccgtcga ggctcatacc accgatagat tcggttgccc agatcgggtc gccagagaac
    37501 agttcatcgt attccggagt acgcaggaag cgaaccatac gcagtttcat gaccaggtgg
    37561 tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc acgttcgatg
    37621 tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt ctgagactta
    37681 acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt ggtagccgga
    37741 ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag agcgcggtgc
    37801 tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc gttttccaga
    37861 tcagcctgca gagaagtgaa ctgtgccagt ttgtctttca tcaggtagtc gataccgtac
    37921 agcgcaacgc gacggtagtc accgatgata cggccacggc catatgcatc tggcagaccg
    37981 gtcagaacac cagatttacg gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc
    38041 tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcggatc cagttcgcgg
    38101 ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat aagagcacgt
    38161 ttcagcggag cttcagtctg cagaccaacg attttctcaa gctgcttgtt gatgtagcca
    38221 gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg
    38281 cggttttcca gtttaacgcc ttccattact ttgtcccaca gggtggtggt cgcttcagta
    38341 gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg aatgaagtca
    38401 cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc tgtggctaac
    38461 ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt atttactgcg
    38521 tacttcgaca accattaatg gtggtcgttt tcacgcaggt aaatgaccca gtatgtcaac
    38581 ccaaccaaca aaccaccacc gataatgttg ccgatcgtaa ccggaatcag gttatcagtg
    38641 atgaaattca tcacggtcag gtgaaaaaaa ttttccggtg cagaaccgac tgcggtccaa
    38701 aattccgggg atgcgaagtc gcggattaca atacccatcg ggatcataaa catgtttgcg
    38761 atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat aatgaacgct
    38821 ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac catcaggttt
    38881 gccaggatac caagacagac ggcctcaata aaagtatggt gcactttgtg gtcggcggtt
    38941 tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga aagccacatt
    39001 aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag ccagtttttc
    39061 gccaactgac cccaggtgat gcgcccactc gccttagcaa caacaatcaa cacggtggaa
    39121 gtaaagagat cggctccgca gacaacacaa agaatcagcc ccagagagaa gcaaatgccg
    39181 ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag
    39241 aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc
    39301 ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat tgcagcagga
    39361 agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt aagtcggcga
    39421 cgagatacta acaaagca
//
LOCUS       DAZFAH010000022        39169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFAH010000022 DAZFAH010000000
VERSION     DAZFAH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.022"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(120..311)
                     /gene="yfgG"
                     /locus_tag="ACHJWB_001074"
     CDS             complement(120..311)
                     /gene="yfgG"
                     /locus_tag="ACHJWB_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4312748.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            329..481
                     /locus_tag="ACHJWB_001075"
     CDS             329..481
                     /locus_tag="ACHJWB_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312749.1"
                     /translation="MSQAVDGTCIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            663..2906
                     /gene="pdeF"
                     /locus_tag="ACHJWB_001076"
     CDS             663..2906
                     /gene="pdeF"
                     /locus_tag="ACHJWB_001076"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4312750.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2945..4486)
                     /gene="ppx"
                     /locus_tag="ACHJWB_001077"
     CDS             complement(2945..4486)
                     /gene="ppx"
                     /locus_tag="ACHJWB_001077"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4312751.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4491..6557)
                     /gene="ppk1"
                     /locus_tag="ACHJWB_001078"
     CDS             complement(4491..6557)
                     /gene="ppk1"
                     /locus_tag="ACHJWB_001078"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4312752.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6728..7366)
                     /gene="purN"
                     /locus_tag="ACHJWB_001079"
     CDS             complement(6728..7366)
                     /gene="purN"
                     /locus_tag="ACHJWB_001079"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4312753.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7366..8403)
                     /gene="purM"
                     /locus_tag="ACHJWB_001080"
     CDS             complement(7366..8403)
                     /gene="purM"
                     /locus_tag="ACHJWB_001080"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4312754.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8728..9354
                     /gene="upp"
                     /locus_tag="ACHJWB_001081"
     CDS             8728..9354
                     /gene="upp"
                     /locus_tag="ACHJWB_001081"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4312755.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9440..10729
                     /gene="uraA"
                     /locus_tag="ACHJWB_001082"
     CDS             9440..10729
                     /gene="uraA"
                     /locus_tag="ACHJWB_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4312756.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10824..11525
                     /gene="hda"
                     /locus_tag="ACHJWB_001083"
     CDS             10824..11525
                     /gene="hda"
                     /locus_tag="ACHJWB_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4312757.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11663..12022)
                     /gene="arsC"
                     /locus_tag="ACHJWB_001084"
     CDS             complement(11663..12022)
                     /gene="arsC"
                     /locus_tag="ACHJWB_001084"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4312758.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12043..13506)
                     /gene="bepA"
                     /locus_tag="ACHJWB_001085"
     CDS             complement(12043..13506)
                     /gene="bepA"
                     /locus_tag="ACHJWB_001085"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4312759.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13719..14780
                     /gene="yfgO"
                     /locus_tag="ACHJWB_001086"
     CDS             13719..14780
                     /gene="yfgO"
                     /locus_tag="ACHJWB_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4312760.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14818..15666)
                     /gene="focB"
                     /locus_tag="ACHJWB_001087"
     CDS             complement(14818..15666)
                     /gene="focB"
                     /locus_tag="ACHJWB_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4312761.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLTGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASDFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15688..17700)
                     /gene="hyfR"
                     /locus_tag="ACHJWB_001088"
     CDS             complement(15688..17700)
                     /gene="hyfR"
                     /locus_tag="ACHJWB_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4312762.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNVYYRYHDDGSDVTATAEYQGEMIDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17730..18143)
                     /gene="hyfJ"
                     /locus_tag="ACHJWB_001089"
     CDS             complement(17730..18143)
                     /gene="hyfJ"
                     /locus_tag="ACHJWB_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4312763.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18136..18893)
                     /gene="hyfI"
                     /locus_tag="ACHJWB_001090"
                     /pseudo
     CDS             complement(18136..18893)
                     /gene="hyfI"
                     /locus_tag="ACHJWB_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18890..19435)
                     /gene="hyfH"
                     /locus_tag="ACHJWB_001091"
     CDS             complement(18890..19435)
                     /gene="hyfH"
                     /locus_tag="ACHJWB_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4312764.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KAALYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19445..21160)
                     /gene="hyfG"
                     /locus_tag="ACHJWB_001092"
     CDS             complement(19445..21160)
                     /gene="hyfG"
                     /locus_tag="ACHJWB_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4312765.1"
                     /translation="MNVNSSSNRGEAILAALKTQSPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWRPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLLYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVTVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21150..22730)
                     /gene="hyfF"
                     /locus_tag="ACHJWB_001093"
     CDS             complement(21150..22730)
                     /gene="hyfF"
                     /locus_tag="ACHJWB_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO4312766.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLEPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22735..23385)
                     /gene="hyfE"
                     /locus_tag="ACHJWB_001094"
     CDS             complement(22735..23385)
                     /gene="hyfE"
                     /locus_tag="ACHJWB_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4312767.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23397..24834)
                     /gene="hyfD"
                     /locus_tag="ACHJWB_001095"
                     /pseudo
     CDS             complement(23397..24834)
                     /gene="hyfD"
                     /locus_tag="ACHJWB_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24851..25798)
                     /gene="hyfC"
                     /locus_tag="ACHJWB_001096"
     CDS             complement(24851..25798)
                     /gene="hyfC"
                     /locus_tag="ACHJWB_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4312768.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25809..27826)
                     /gene="hyfB"
                     /locus_tag="ACHJWB_001097"
                     /pseudo
     CDS             complement(25809..27826)
                     /gene="hyfB"
                     /locus_tag="ACHJWB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27826..28443)
                     /gene="hyfA"
                     /locus_tag="ACHJWB_001098"
     CDS             complement(27826..28443)
                     /gene="hyfA"
                     /locus_tag="ACHJWB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4312769.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDGAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEDPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28696..29166)
                     /gene="bcp"
                     /locus_tag="ACHJWB_001099"
     CDS             complement(28696..29166)
                     /gene="bcp"
                     /locus_tag="ACHJWB_001099"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4312770.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29166..29738)
                     /gene="gcvR"
                     /locus_tag="ACHJWB_001100"
     CDS             complement(29166..29738)
                     /gene="gcvR"
                     /locus_tag="ACHJWB_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4312771.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29884..30762
                     /gene="dapA"
                     /locus_tag="ACHJWB_001101"
     CDS             29884..30762
                     /gene="dapA"
                     /locus_tag="ACHJWB_001101"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4312772.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30779..31813
                     /gene="bamC"
                     /locus_tag="ACHJWB_001102"
     CDS             30779..31813
                     /gene="bamC"
                     /locus_tag="ACHJWB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4312773.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32026..32739
                     /gene="purC"
                     /locus_tag="ACHJWB_001103"
     CDS             32026..32739
                     /gene="purC"
                     /locus_tag="ACHJWB_001103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4312774.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32893..33120
                     /gene="vapB"
                     /locus_tag="ACHJWB_001104"
     CDS             32893..33120
                     /gene="vapB"
                     /locus_tag="ACHJWB_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO4312775.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33120..33518
                     /locus_tag="ACHJWB_001105"
     CDS             33120..33518
                     /locus_tag="ACHJWB_001105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO4312776.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33665..34528
                     /gene="ypfJ"
                     /locus_tag="ACHJWB_001106"
     CDS             33665..34528
                     /gene="ypfJ"
                     /locus_tag="ACHJWB_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4312777.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34543..36558
                     /gene="tmcA"
                     /locus_tag="ACHJWB_001107"
     CDS             34543..36558
                     /gene="tmcA"
                     /locus_tag="ACHJWB_001107"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4312778.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36632..37330
                     /gene="ypfH"
                     /locus_tag="ACHJWB_001108"
     CDS             36632..37330
                     /gene="ypfH"
                     /locus_tag="ACHJWB_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4312779.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37440..37640)
                     /gene="ypfN"
                     /locus_tag="ACHJWB_001109"
     CDS             complement(37440..37640)
                     /gene="ypfN"
                     /locus_tag="ACHJWB_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4312780.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37668..38795)
                     /gene="dapE"
                     /locus_tag="ACHJWB_001110"
     CDS             complement(37668..38795)
                     /gene="dapE"
                     /locus_tag="ACHJWB_001110"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4312781.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38799..39155)
                     /gene="yffB"
                     /locus_tag="ACHJWB_001111"
     CDS             complement(38799..39155)
                     /gene="yffB"
                     /locus_tag="ACHJWB_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4312782.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9583 a        10050 c        10293 g         9243 t
ORIGIN      
        1 tcaggacaaa gacgtaaaat ttaagatgaa agaatatctg ccgctttgat aattccaaag
       61 agcaaaagca tccgacagcg aagtgccgtt atgttctgtg aaagtggaga aggtaaccgc
      121 taacgttgta ccggtgattc gacggagagt gtggattgct gagcttcttt tttgctctga
      181 tggtgctgcc aggcaccgac ggacgagtag ataaaacggc caaagaagaa gataaagctg
      241 atgagcagta cgatacgggt catgcgactg ttaaatcggt gtcgttttcg catactggtt
      301 gcctgactca caaaaggttc cttgaagtat gtcccaagct gtggacggta cttgcattaa
      361 ggcacaacag gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag tggatacata
      421 ttttaatgtt atggaagtta atttaattat ttacaatgat gatgtaataa tgatgaatta
      481 acatgataaa cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga
      541 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
      601 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      661 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      721 tcttgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      781 ccggtgaggt ttttctcttt tatctgcctc tggcactgat gatcagcatg atgatgtttt
      841 tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc
      901 tgggttttta cgaaacgcta tcattaacgg ctaattttat tatcattatc attctctgtt
      961 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
     1021 taatttccca gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt
     1081 tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc
     1141 cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg
     1201 tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt
     1261 attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc
     1321 tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
     1381 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc
     1441 gctatggtta taagctgatt tctctgctct gggcggtcgt gttgatgatc agcatccaca
     1501 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1561 ccagttatct ggtattctct tttattgtca attatatggc tgtactggca acccgtcaga
     1621 gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa
     1681 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1741 gcatccctgg catggaaatg ctggttaaga actatggcat catgctgcgg attcaataca
     1801 agcaaaaact ttctcactgg ttgtcaccct tgctggaacc gggtgaagat gtttatcagc
     1861 tttcgggtaa cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattaccg
     1921 cactggatag ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc
     1981 agattggcgt cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg
     2041 gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc
     2101 gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac
     2161 tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc
     2221 gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatca
     2281 gccccgatag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt
     2341 gggtcattga gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc
     2401 gttttgctat taatctgtct ccaacctcgg tatgtcaggc tcgttttcct tttgaagtca
     2461 gtcagttgct ggctaaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa
     2521 gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag
     2581 gctgccagat tgcgattgat gatttcggca ccggctacgc cagctatgca cggcttaaaa
     2641 atgtgaatgc cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata
     2701 gtctggatta tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg
     2761 tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg
     2821 attatatgca gggttatctt attggtaagc cgcagccgtt aattgatacg ttgaatgaaa
     2881 tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa
     2941 agcattaagc ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca
     3001 cgccttccca gtattcttgc tccttttcca gatcaagcag taccaacgca ttctgactaa
     3061 accagtcatg cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg
     3121 gcggtgtggt ggttgcctga cgttgattgt tgagtaatac gccaaggcgc aatagctgaa
     3181 tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagtttaa
     3241 tcgctttacg gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac
     3301 ccggcaagtc actgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga
     3361 tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat
     3421 gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca
     3481 cccgtcgggc ctgctcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac
     3541 tacgcacatc ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg
     3601 ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg
     3661 ccagtccagg aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg
     3721 cgaaattacg gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta
     3781 tcccgtcttt ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag
     3841 cgcccattgc cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt
     3901 gtgctgccgc catacgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaagt
     3961 aaagctgggc aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat
     4021 tttcaccaat caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt
     4081 tctccggttg ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa
     4141 taatttcaat cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca
     4201 gcgcctgacg cagcgtatgg gtgccgacta tacagacgct ggcaggagaa aacccttgta
     4261 gccgttcggc aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca
     4321 tattatctgg ccccaggccg tccgccagat gcactcgctg tttcaggcgg ccaataatct
     4381 gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga
     4441 ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt
     4501 tgttcgagtg atttgatgta gtcgtaaatc gccaactgcg cccgtacttt gcggcgattg
     4561 ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg
     4621 ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggt
     4681 gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag
     4741 acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca
     4801 acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac
     4861 attccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca
     4921 accaggcctt tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc
     4981 tgcgcgttgg cgatctcgcg gtccaccatt tcatacaaca gacggcggga gttttgcggc
     5041 gacaccatta aataatcaaa tgtcaccgga cggtatgggt tttcaataaa gttgaacacc
     5101 cgccgtactt cgttagtgat gcgcgcatct gcggtcagca acgaatagtc agtataaaga
     5161 cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cataacgcac cacttcaccg
     5221 ttttctttac gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag
     5281 ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtcg
     5341 aaacgcgcct gtaactcaac caccacggtg actttcttac cgttatgtgc ggcgtggatc
     5401 atcgagtcga tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt
     5461 acgctcgggt cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtgtgataa
     5521 ggataataga gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct
     5581 ttatcaaacc aaatgtggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg
     5641 acattgggga aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag
     5701 tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg
     5761 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc
     5821 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
     5881 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg
     5941 cagtaacgca gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg tgcttctggc
     6001 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg
     6061 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
     6121 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
     6181 taatgacgaa gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
     6241 tggttacgcg ccatctccag cagcagctcg ttgtagaggc cgtcgaattc ctgatcggct
     6301 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt
     6361 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
     6421 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
     6481 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
     6541 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
     6601 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
     6661 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     6721 cggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg
     6781 cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa
     6841 tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata
     6901 ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca
     6961 ccgatgtacc gtgctcttca tcaccatttt ccagcgcttg acgatgggtg tgtaatccgg
     7021 gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa
     7081 acgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt
     7141 cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga
     7201 gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat
     7261 tgctgaaaac tgcccgtacg gtgcctttaa ttttgttggt tttacaggcg tcaataattg
     7321 cctgtaaatt acttccgtta ccggaaataa gcaccacaat attcattatt cgataaccac
     7381 gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc
     7441 attgagcagg gcgagggctt tgtccacttc cggagcaggc agggcaataa tcatcccgac
     7501 gccgcagttg aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca
     7561 gttgaacact tccggccact gccaggaaga ttcatcaatc actgcctgag tattatctgg
     7621 caatacgcgt ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac
     7681 atcgaccttt tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag
     7741 ataatcggct aatggcttac catcaagttg ggtggtttgc ggatcacaac cgctgacttc
     7801 aagaattttg cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat
     7861 cagcacatcg ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc
     7921 aacgcagaaa cccgcgacat cgtaatcctc accgtgatac atccccggca tttctgccgt
     7981 ttcgccaccc accagtgaac agcccgattg cagacatcct tccgcgatac cgctgatcac
     8041 cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg
     8101 ctctgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt
     8161 gtcgtgacgt tttaagtcca ttgccagacg cagcttggta cctacgccgt cagtgccgga
     8221 aaccagcacg ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag
     8281 accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag
     8341 agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt
     8401 cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa
     8461 cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttgccca
     8521 ggcgcgcggc gaaagaagac ttgtaccagg gtaaaggtta gttttcggat ggaataatct
     8581 tctttcataa ccatttgaat ataaaataac tttatctcaa gccgttatca ttttgactaa
     8641 agtcaacgaa aagaatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt
     8701 gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc
     8761 aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc
     8821 gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa
     8881 gtaactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt
     8941 accgttgtgc caattctgcg tgccggtctg gggatgatgg acggtgtgct ggaaaatgtt
     9001 ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta
     9061 ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca
     9121 atgctggcaa ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc
     9181 agcagcatca aagttctggt gctggtagct gcgccagaag gtatcgctgc gctggaaaaa
     9241 gcgcacccgg acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga
     9301 tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata
     9361 aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca
     9421 taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact
     9481 tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct
     9541 ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac
     9601 gctgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc
     9661 ctttatttca ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt
     9721 tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg
     9781 ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct
     9841 ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc
     9901 agactccaaa accatcatca tctctattac caccctagcg gtcacggttt taggttccgt
     9961 gctgtttcgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg tgggctacgc
    10021 gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc
    10081 gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc
    10141 ggcgttagtg gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa
    10201 aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcctgtcgac
    10261 cgtgatctcc ggcttctttg gctctacgcc aaataccact tacggagaaa acattggcgt
    10321 gatggcgatc acccgtgttt acagtacctg ggttatcggt ggggcggcga ttttcgctat
    10381 cctgctttcc tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat
    10441 gggcggcgtt tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat
    10501 cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat
    10561 catcggcgtc agtggcgcga aggtaaacat cggcgcggcg gagttaaaag gtatggcgct
    10621 ggcgaccatc gtcggtatcg gcttaagttt gatcttcaaa ctgattagtg tgctgcgccc
    10681 tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg
    10741 ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt
    10801 gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc
    10861 ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggctg
    10921 cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag
    10981 gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg
    11041 cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta
    11101 tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt
    11161 gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa acacgattgt
    11221 tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc
    11281 gactcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg
    11341 cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct
    11401 tgctgaagcg gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc
    11461 gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt
    11521 tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt
    11581 taatccatag tattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct
    11641 tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac
    11701 caatccgcgc tttgccattc gcgaccacaa tcgggcgctc catcagcttc ggattatcca
    11761 ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat
    11821 aaagatcctc tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca
    11881 aatcgcgcag cgttgccgca tcggcgggtg tctcaaggta gagtaccact tctggctcca
    11941 cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt
    12001 agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg
    12061 cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg
    12121 caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag
    12181 tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag
    12241 cgcggcttcc gcctgtgcca gtaaatccca gccgttgctg tcatctttat tattaaaggt
    12301 gtagcgattg agaatattgg ccgcttcttg tggttgaccg ccttgcagat aagcgtttgc
    12361 caggttgagc tgcaacacag gattggtgcg caaatcacgg gcatttttca ggcgattgat
    12421 tgcctcattg gctttgtttt gcccaagatc gatatcggta gccagatcga gataccatgc
    12481 gttgccaggt tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt
    12541 ggcttccatc gcctgtaaag cacgaccata ttgtgctgct cgttgctgac gaacgtttcc
    12601 tttcgcccat tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc
    12661 cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcgggcgcat
    12721 ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcaataa
    12781 aatttccggc ggacgcgagg agtaacgcgc ctgatcgagc aatttttcga ggaaggtcgg
    12841 catcgcctgc ggatcgaatc ctgagcgttg cagcacctga ataccaatgc ggtccgcttc
    12901 ctgttcattt tgctgggtga aactgatcat cccctgacgc gttcccgcca gtgtaccggt
    12961 cagcgccgcc atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc
    13021 gacccaggtc agcggcgcgc tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg
    13081 ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata
    13141 acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc
    13201 gtcgttgttg atcagaaaaa aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag
    13261 acgcatcccc agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct
    13321 gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt
    13381 gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa aagccggggc
    13441 tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt tcaactgcct
    13501 gaacataacc tctatcctgt atttctgaag atagtcattt gaccgatact gttaaatatc
    13561 gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc
    13621 agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg
    13681 gatcgcaatc ccgctcttga ggttcaggga aggtttttat gctcgaaatg ttgatgcaat
    13741 ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg
    13801 gatttggcat tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc
    13861 tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct
    13921 gggcgacgtc gattgtattg gtggtttttg tcggtatatt gctactgatg gcgttcgtgg
    13981 tactgcccat cgcctggcaa cagggcatct acttaatccg cgatatgccg gggatgctca
    14041 ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggacgcgg
    14101 gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg
    14161 tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc
    14221 tggtgccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg aacgccgttc
    14281 gtcgggtgct gccgcgtaac cgtgggctgg caggacaggt gtggaaggag atgaatcaac
    14341 aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtggtaggg atcgccacct
    14401 ggctggggtt cttgctcttc gggctgaact attcgctgct gctggcggtg ctggtcggct
    14461 tctcggttct tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg
    14521 cgctattcca gtttggcgca ggcacggaat tctggagctg cttcgcggtg tatctgatta
    14581 ttcaggcgct ggacggcaac ctgctagtac cggtgttgtt ctccgaagcg gttaacctgc
    14641 atccgctggt gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg
    14701 tattcttcgc cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg
    14761 ggcaaatcgc gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca
    14821 ggattcctga cgtaaataaa tagcccgata acaaatactc accagcactg caccgccgat
    14881 aatattaccc agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc
    14941 cggaaaattg tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg
    15001 aatggcaatg gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agtcactggc
    15061 gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca
    15121 ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga
    15181 ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat
    15241 cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc
    15301 tgtcagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aactaataac
    15361 gccccgactt tttgccatta ccgtcattac cgacgaggtg aacaggttgg tgccgcaaac
    15421 cgccagcaaa ataaagccaa gtgtaaagca aaggccgccc accagatggg ttaatgcctg
    15481 tgaagacggg gcatcggcga taactgaaag gtaaaaagta aatccgatcg ccataaacac
    15541 gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc
    15601 aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag agagtttgtt
    15661 tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga ttcccagacg
    15721 ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg cgccacgggg
    15781 cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac gttcttcttc
    15841 gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc
    15901 ttcatttagc gtcggcagta aacggctttg tcggacatta agatgtaaat tcagactgtt
    15961 accgcgagtc agcagtaccg ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg
    16021 ccagtcccac gacatcaact ggcgtagcgc ctcggtcggg atggcgtcaa ttgaacgatt
    16081 catatggcgc gccatttttt gcgtgaaatg ctttgctaaa agagggatat cttccggacg
    16141 gtcgcgtagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga gatcgctgcg
    16201 aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa tgactctgac
    16261 atttaccggg atcgttctac tcccgccgag acgctcaatc tcccgttcct gcaatacgcg
    16321 cagcagttta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa acaacgtgcc
    16381 gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa acgccccttt
    16441 gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc agttgatttt
    16501 gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga tcacctcttt
    16561 gccggttccg gtttcaccgc aaatcaacac cgtactgtcg ctcttcgcca caatatctac
    16621 ctgctggagt aggtcttcca ttgcctggct ttgatagata atgtcaccga cgcccagatt
    16681 cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct gcaaatcggt
    16741 cattctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg
    16801 tagtagctgg cagttttcct cactaaagag agtggaagag gtatgcgcca gcaacaatgc
    16861 acccggtgta tggttgccaa aggtaagcgg tataaggagc gcagattcgc agccgttaga
    16921 tgcaagtaag agtaataacg ggtcgcgttg ccagagaaac agatcatctg cacggtgcgt
    16981 caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt
    17041 gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc tgaacttctc
    17101 gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat
    17161 ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt
    17221 gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgccacctg
    17281 ctgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg ccagccagtt
    17341 aaacagtgtg caatcctggg gagtaaaacg gcagccgtgg gtgcgaataa agcgtagctc
    17401 cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgatca tctcgccctg
    17461 atactctgct gttgccgtca catcgctgcc atcatcatgg tagcggtaat agacgttgct
    17521 tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca gcgggatgaa
    17581 ggcgcgtaat aaagacgcct taccgtgttt ctgcgctaac cgctccgcga gcattccgtt
    17641 taccgcttca attgttattc cttgcggcgg ggcaaacatc gcctcgtctg acatagccat
    17701 caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat cagatagatg
    17761 gccggttcct gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcttgccac
    17821 acagacgtcg cgtcatctgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg
    17881 gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg ggcttgctcc
    17941 tcttcgacca gtgcaagcca atcttcgtat tccgtcagtg ggcagcggaa ggcgacattc
    18001 agacaatcaa tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgagag
    18061 ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca
    18121 atttccccgc actcttcagt catgtaaccc cctgagtacg cgaaagagtt gctgaacgat
    18181 actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg
    18241 atcgtcgctt aaatggcgca ggagccgatc gcaaatttct cgcccctgac gatagcccgc
    18301 cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct gtggccacaa
    18361 cggttgcata gtcaccccgg tgggatcgcg ataatccaca gcgtgaatct tctgttgcag
    18421 caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg
    18481 gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg
    18541 gaaaataccg ccgccgacac cgcacgcgcc gtaggaaaca cagatttatg atcgggggca
    18601 gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc agtaaataac
    18661 aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc caaaacgttc tgcgtcgaat
    18721 acgggggtga tggcagcaaa aatttcgatt tcacaggcgt tgcagccacc gcaatcgacg
    18781 cgataaacgt aggctgagcg acggatatcc tgtagcaaat gccgcttcat attttgcgtt
    18841 tgctcgtcca gcgtgatggg ctggctgaca tattgcgtaa gcactggact catagctgct
    18901 ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt tgtttgcact
    18961 ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca ttttgttgct
    19021 gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg cgttcgcaac
    19081 ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag agcggcttta ttggtgacgg
    19141 tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct tcacaacgtc
    19201 cgcagtaaat acaacgcccc agatagagct gccaggtgcg cgaattttgc tggtcgtcgg
    19261 tctggatagt cagcgcattt gccggacaag cacaggcgca ggcaccgcag gcaatacatt
    19321 ggctgggcat caggtccggt ttcccgcgaa agcctgggct gacctccagt ggcgcgaagg
    19381 gatatttcac cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc agcatcttct
    19441 gtccttactt cagcggcgaa cggttacgat caatgccgta gcgttcgatc tctttatacg
    19501 gcacggtttt tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg gtacaggagt
    19561 agcagggatc aaggctaccg ataatcagcg gtgcgtcaga aacggtattg ccgcgcagca
    19621 tgtaacgcaa caccggccag ttggcgtagg tggcggcacg gcagcgccag cggaacaatt
    19681 tttggttatc accgagcatg ctccagtgca cgtcttcacc gcgtggcgct tcaacaaagc
    19741 ccagcgcgaa tgcgtgaggt ttatagctaa agccttcggt cagcagtggg gtatccggca
    19801 tgttgtcgag agcaaattcc agcattgcca gcgaatcaaa cgtctcttta acacggaccg
    19861 tcacgcggga gaaaacatcg ccgccggtaa aggtaaacag tgtttttgga atattgccgt
    19921 agtcggcgta ggggtgatca aagcgcaaat cacgggcaaa accactgccg cgaatcagcg
    19981 gccctacagg gctataatca cgggcgattt gtcggtcgag aatgccaatg ccctgagtgc
    20041 gttgttccat attcggcgta gcaagcagca tctctaccag ctccgacacg tcggcgcgca
    20101 tctcgcgcac cagtttcagc gtttgcagac gttgctcttt gagaatatcg cggcgaacac
    20161 caccaatcag attcagaccg taggttttac gcgacccgat cagcaattcc gccatcgtca
    20221 tcgacttttc ccgcacgcgg aaaaattgca taaagccggt atcaaaacca acgaaatggc
    20281 aggagaggcc aaggttaagc aaatgactgt gtagccgttc gacttccagc agaatcgagc
    20341 gaatagtatg tgctcgttgc ggcacctcaa tccccagtgc attttcaacc gagttggtat
    20401 aggccacact gtgggcaaaa ccgcaaatcc cacacacgcg gtccgataag aaggtcactt
    20461 cgttgtagcc catccgcgtt tctgccagtt tctccatgcc gcgatggaca tataacaggc
    20521 ggtaatcagc atcgacaatt tgctcgccat ccacaaacaa gcggaagtga cccggttcat
    20581 cggaggtgat atgcagcggg ccgacaggga tcacccgcgc atcgctgttg ccctcattga
    20641 taaacgcata cgtttcggaa tctgtcgtcg gttcagggcg cagtcgataa tccatcgcat
    20701 ctttgcgcag cggatgcata tcttccggcc agtcatcggg caacaccagg cgacgctgat
    20761 ccggcaggcc aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg ccccagaccg
    20821 cggcagggac gcgcggtgtg actgacggaa actcccgact gtcggcatcg accagtgcct
    20881 tcaccacgat ccagcatttt tcggcccctt ccattgaaag ggcataataa accgcgtaat
    20941 gaccgttaag tgtccgctcg tcgttgccaa acaggactgg acgccagcca tcatgttgat
    21001 aataaagata atgtacaacg tcaggcagca gattgatttt caccgtaatg gtgacctgtt
    21061 caggcgtttg tcgctcttca tccagcaccg cgccggggga ctgcgttttc agggcggcga
    21121 gaatcgcttc gccacgattt gacgatgaat taacgttcac tgtgtttctc cgaaacagat
    21181 gcggtgcctg aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag gtcgtgcgtc
    21241 cctgagagga ctatagtgga agcgcccgcc aggatcctga tgacaggttg tggaatatgc
    21301 gttcccatcg ccagcatcat gaccagcaga atcaccattg gcgaggtggt caaccagccg
    21361 agatcacccc ggttaacggc ctgcggcggt ttcgccatta acacccgcgc tgccatccgt
    21421 accaggcccg ccagcaccag cgttaacagc aataacagca ggacgataat cagcaggtga
    21481 ttacgtgcca gtccggcggt aatggtcata aattcgctaa gaaaaatgtt gaagggcggc
    21541 atccctgcca gcgccagcgc accgccgcca aacagcacgg cggtaaatgg catgattttg
    21601 agcatcccac agacgacgtt gagatcgcgc gtgccgtact tgagcagtac attgccggaa
    21661 ccgcagaaca gcagcgtttt tgccagactg tggtttaaga tgtgcagcag cgcggcaaaa
    21721 attcccagcg gcccgccaat gcctagcgcc accgcgacca gccccatgtt ctccacgctg
    21781 gagtacgcca gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc cgccacggca
    21841 accgacaaca tgccgaagat gagcaacaac cggttgggga aatcgctgcc gatggcctgg
    21901 cagataatga tgtaatagcg aatcagcacc aacagcgcgc agttcagcaa tacggcggag
    21961 agcagggcgc tgaccggact cggtgcttca ctgtgagcat ccggcagcca ggcgtgcatg
    22021 ggaaatagcc cggttttggt accaaagcca attagcacaa acacaaaggc caacagcatt
    22081 aatgttggtt caagcaagga cgattgctta agaacctcgc tccagaatat cgccatttct
    22141 gcctgcggca taacgctggc ggcgttggcg tataccagca cggtaccgaa cagaccaaaa
    22201 gcgacaccaa cagtacaaat aatgatgtac ttccatgctg attccagcga tgaacgctga
    22261 ccgtaaatgc ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc agcccacatc
    22321 acaatcaggt tattgctggt aacaaccagc agcatggtga acaaaaacag atggaagaaa
    22381 ccgtagtaat cgcacagcgt aacgggtgaa agctcgccgt gtgccacttc atgacgcatg
    22441 tagccaatcg agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa
    22501 ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg
    22561 gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat
    22621 acagtcaccg tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa
    22681 aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc
    22741 cttcagcgcg gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc
    22801 cagtaacacc atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag
    22861 ttccggtgct cgccaggcaa gaagcgccag caccagatgg gagccgtttt ccatcaggca
    22921 gtaaccaaaa atttgccgca ggatattgcg ctggctgaca atgcacagca ggccaagcag
    22981 aaaatgacct aacgctaccg ccagcgccgg tttcagcccg gtagccatcg gtagcttcac
    23041 gggctgaacg acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg ccatcattgc
    23101 cggaccgaat aacgcttttt ccgggatgtt ctggggaata tttcgtgcag cgtaagtcat
    23161 gattaacggt accagcagca ctttggtgat aaaggcgctg gcggaccaga tcagcagttg
    23221 ctctgcggcg aacaggcacg agagagtggc gaaaatagac accagcacca gcgactggca
    23281 ggcgtaaaat ccgcatgaca ggcgatagct tttgacgcta atcacaaaca gcgatgtcag
    23341 catcatcagt cccgccagat tatttacgat catagaaccg gtcattcatc atctccttac
    23401 tgcaaccagg tcgcggcgat tacgctggaa atcagcgaca tcacaatcag tacaatcaac
    23461 accaggcgca ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct cggtttgcca
    23521 ggcacaacgc gaccaaacca gcgaataaac caggcgaaac tggcgaccga ttcaatcatc
    23581 agcagaatca tggcgggcag caggatccag tactccactg acaacgcaaa accggcagca
    23641 aacagcggga atttactgaa gaagccgttg aacggcggca cgccggtaat cgccagcgct
    23701 gccacgcaga aacccacgcc tggcaacggc agggtgtgca atacgccacg cagacgcggc
    23761 aacaagcgcg tgccgcagct gtaactcagc gcacctgcta caaggaaaaa caggctttta
    23821 gcgaacgcgt ggttgacgat gtaggcgatg ctaccctcca gcgccagccg cgagccgaag
    23881 atggagagcg acaagccgaa gaacatccag ccaagttgag tgatggtcga ccaggccagc
    23941 aaccgcttca tatcctgctg tggcaaatac atcagaaagc cataaagaat ggtgaccagt
    24001 gccatgacca tgccaacgcc gccaatcaca tgcgggatat tgccgccgtc gataattgcg
    24061 cgggcaaaaa tgtaaacgcc cactttcacc atcgatgcgg cgtggagata ggcgctgatc
    24121 ggtgttggcg cttccattgc atccggtagc cacgcttgca tcggtagctg ggccgatttc
    24181 ccccacgcgg caaacaggat cccgccataa accagataac gtgcgtcgcc gtgtaactcg
    24241 ctcattgcgc taagcgcaaa cgttccagtc tgcaaaaaca gcgtggcggc ggcaagatac
    24301 aaccccaacg aaccgatatg ggtgataagc aacgctttta gtgctgaacg ctgcgcttta
    24361 tcgctctggt aataactgat caacgcccag gagcagccgc cctaatttca aaaaacaaca
    24421 actgaccgag cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca
    24481 aaaatgcgta ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat
    24541 aacccgtgga gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg
    24601 tactcacgcg atcaatgacc aggccaaaca atgccacatc ccccacgttg accaacgtca
    24661 acgtgacggc aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg
    24721 tggtcagtgc ggcgaacaaa acccccattc ggcggcccga cgttgtggcg aaaacgaaac
    24781 gaccagtgcg ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg
    24841 tcagtgctcc ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc
    24901 caagccaggt cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga
    24961 ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca
    25021 ggcaggcgag agaaagttct tgcgcgcgcc caaagggcag aaacagggcc acaaacagtg
    25081 ctgccatcac gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg
    25141 aatattcggt caacgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa
    25201 tttttcccat ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta
    25261 gcggcgagtt ccagcccgtt gccagcgtat tgctgatcat ctcgatatgc gtggaacctg
    25321 ctatcagcgc caataccagc agtgagagaa tgagcatggg ttcgaccaga atgccgagcg
    25381 tcaactcgcg actggcaccg actcccgcaa acgggcttcc ggtatccagc ccggaaagag
    25441 caaagaaaaa acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg
    25501 caaaagggga aacggtaata aaaagtggta gggccatcgc cagcaccagc atgctgctga
    25561 ttaataccca cggcatcagg cggaacatca gacctgaaga tataggcgca acttcctggc
    25621 gtttaaacag tttgtggata tcgcgataat cctgccagat ccccggcccg cggcgggagt
    25681 gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag cggggttagc agcagcagaa
    25741 tcacggcctg tgctaacgca aaaatgacca gatatccgtc gcaaagagtt tgtctcatgg
    25801 tgatttcctt agacggcaat agcgattagc agcacaacca gtgcggcgac cacatacagg
    25861 caatagagac gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg
    25921 gcgctcacga tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg
    25981 gcggtgacgt gcgcaagatc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga
    26041 cgaaataacg cagagaagac cacacgcagc ggctgcatca cgccattgcc tgacggagcc
    26101 atcgcgtttt cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg
    26161 cggcgcgaac gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac
    26221 agcaacagta atgaaggagt gacccgtgta tgaaacgtgc cgggtacaag tgctattccg
    26281 ctggcgacag tgacgggagg ggtattggta aacgcatggg caatatgcat tatcttcggt
    26341 gccagccaac tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg
    26401 acgatcatcg tatttggcac ttcctgtgcc tcttcagcgt gtgtactgcg cggcgcacca
    26461 cagaaagtaa taccgtacat tttgacgaag cacattactg ccagcccacc agtgactgcc
    26521 agcattacca tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt
    26581 gagaacagcg actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct
    26641 gatatcgcga ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt
    26701 gcccccattt tttccatgtc gtgggtatgc aaacgcgaaa taatcgcgcc cgcgccgaga
    26761 aatagcagcc ctttgaacag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca
    26821 accacggtga gcagcgggtc gtgcagtgac agaccgtcca tcgccacacc gactgccagc
    26881 aaaataatgc cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc
    26941 gccagcgcat atagcacgcc caggagcgcg gagattgcgc cgatcgccat caccagaatg
    27001 ccccaccaca gaggcaaacc cgtttgcgcc agcagatcca tcgctacttt caggatgccg
    27061 aaaataccga ttttgaccat tacgccagac atcaacgccg aagcgtgcga tggtgcggca
    27121 gggtgagcgc gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg
    27181 aaaaaggcca gcagaaacac cgccgacgcc agccccggag aaagtgaaag cgtgcgaaac
    27241 tggcaaaatc gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc ataatcagca
    27301 ccgatccggc gtgggcgata aaaaagtaga gcatgccagc gttgatcgac gttttatcct
    27361 gcctggcaat gaccagaaac caggaagaca gcgacatcat ttcaaacagc acgatgaacc
    27421 aaaaagcgtt gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga ttcataaaga
    27481 agcccatcgc cgccgcgcct ttgccctcgt attcgcgcat ataagtcaat gaatagagcg
    27541 aacaaaccac caccagcaag gagataacca gcaccataaa tgccgagagg ctatccattc
    27601 gcagggttaa atcggcaaag tcaaaagggg tggcaaaacg ggcgactaaa gttacaccag
    27661 catgtaattg cgtaattccg ctgatgatgc caatcacgcc gcccaccagc gatgtgatgc
    27721 cggaaagctt aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc agactggcaa
    27781 acagatagag gatcagcgac caggttaata attgcagggc atccattagt gttgctcctg
    27841 agtgagggaa aggggatcct ccccgccatt ggcaaaccag cttgcggcaa ggcgctgttt
    27901 ttctttcatc tgacgttgca ggctatcgtc ggtgatcagc cgtaacgcct gattcgggca
    27961 agcgcgaacg caggccggat cttctggcaa gaaatcacac aggtcgcatt tcaccgcgac
    28021 ggtctggaca cccggttccc agcgcagcaa cgcatgttgg ggcagcacgt tttcttcgcc
    28081 gtctttgagt gagccttcag cctgaaaaac atattgcgca tgggcattca ccggacggct
    28141 tcctgaagcg ctgattgcgc caaatgggca gaccacggcg caaagcttgc agccaataca
    28201 gaggctttcg ttgagttgga tcgcaccatc cctctgagag atggcattga ccgggcagac
    28261 ctgcaggcaa ggggcttcct cacagtgatg acacacgaca ggggcagtga ttgttgacgt
    28321 cttcgccagg gccaggcgcg ggtgttgctg taaaccttgc gttttatgca cgtccgaaca
    28381 ggcagcgaga caggtattac atcctgtaca ccacagtggt tcggccacca caaagcggtt
    28441 catgtcggct ccttgaaaat gatatgaaac attgcatttc atattgcata aaggatgcca
    28501 ttctgattaa ctcaatatat tcaataagtt atgtattgac tggattctga acggtacaaa
    28561 aatgatggtc attaacgttc agcaaaaaat ggattcgaca cccgtgtcgc aggggaaagc
    28621 gagaatgtga tcttgatcac gagaagtgag gtatgctggc cgcaagcgca gccagcacgg
    28681 aatggagcaa agtaatcagg cgtgttcttt cagccagttc agcacaacgt cgtggtgatt
    28741 gctggttttg aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa tcaggaagct
    28801 gatgcgatga atgccatcgt aggttttgcc catgaaggac ttttcacccc agacgccgaa
    28861 ttgttcgcac acctggtggt cctcatcaga caggagcgta aagttaagca gctctttttc
    28921 cgcaaaacgg gagagttttt cgggtttatc ggtgctgata cccagcacat caacgcccgc
    28981 ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac agccgggggt
    29041 catggctttc gggtagaaat aaaccagaac acgctgtccc tggaagtcgg tcaaattaac
    29101 ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg ctttcagtgg
    29161 attcattact taactccatc ctgttcatca tgttgggaat aattgacgac gttaatactg
    29221 ccttgtgcat tgagttctgt acatagggct ttgaacgctt gctcaatatt tgctgcgtcc
    29281 gcagatgcgg ggctgtgggc ggttatctga atatgcaact gcgcagcccg ttcattttca
    29341 gcaggttgcg tgcgcgacac cagctccgca atgttcatat gatgcgcgtc gaaaagtgct
    29401 gtgaagcgtt caattaaatg cggggagtct gccacatcga cctgaaccca gacagatgct
    29461 ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag atccagttcg
    29521 gcacctttca acggtaacgt tgattcaatc agagtaatgg cattccatga accggaaagc
    29581 agcataataa acgtgaactc ttctcccagc atcgccaggc gactgtcttc aatattacag
    29641 ccgcaactac tgacatgacg ggtgatggtg ttcacaattc cagggcgatc ggcacccaac
    29701 gcagtgatca ccagataatg ttgcgatgac agtgtcaaac tggttattcc tttaaggggt
    29761 gagttgttct taaggaaagc ataaaaaaaa catgcataca acaatcagaa cggttctgtc
    29821 tgcttgcttt taatgccata ccaaacgtac cattgagaca cttgtttgca cagaggatgg
    29881 cccatgttca cgggaagtat tgtcgcgatt gttactccga tggatgaaaa aggtaatgtc
    29941 tgtcgggcta gcttgaaaaa actgattgat tatcatgtcg ccagcggtac ttcggcgatc
    30001 gtttctgttg gcaccactgg cgagtccgct accttaaatc atgacgaaca tgctgatgtg
    30061 gtgatgatga cgctggagct ggctgatggg cgcattccgg taattgccgg gaccggcgct
    30121 aacgctactg cggaagccat tagcctgacg cagcgcttca atgacagtgg tatcgtcggc
    30181 tgcctgacgg taacccctta ctacaatcgt ccgtcgcaag aaggtttgta tcagcatttc
    30241 aaagccatcg ctgagcatac tgacctgccg caaattctgt ataatgtgcc gtcccgtact
    30301 ggctgcgatc tgctcccgga aacggtgggc cgtctggcga aagtaaaaaa tattatcgga
    30361 atcaaagagg caacagggaa cttaacgcgt gtaaaccaga tcaaagagct ggtttcagat
    30421 gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg atttcatgca attgggcggt
    30481 catggggtta tttccgttac ggctaacgtc gcagcgcgtg atatggccca gatgtgcaaa
    30541 ctggcagcag aagggcattt tgccgaggca cgcgttatta atcagcgtct gatgccatta
    30601 cacaacaaac tatttgtcga acccaatcca atcccggtga aatgggcatg taaggaactg
    30661 ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac caatcaccga cagtggtcgt
    30721 gagacggtca gagcggcgct taagcatgcc ggtttgctgt aaagtttagg gagatttgat
    30781 ggcttactct gttcaaaagt cgcgcctggc aaaggttgcg ggtgtttcgc ttgttttatt
    30841 actcgctgcc tgtagttctg actcacgcta taagcgtcag gtcagtggtg atgaagccta
    30901 cctggaagcg gcaccgcttg cggagcttca tgccccggct ggaatgattt tgccggtgac
    30961 ctccggtgat tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta aggcgctgga
    31021 cattcgtcca ccagcccagc cgctggcact ggtttctggc gcgcgtaccc agttcacggg
    31081 cgataccgct tcattgctgg tggaaaatgg tcgtggcaat actctgtggc cgcaggtggt
    31141 tagcgtgctg caggcgaaaa actacaccat cacccaacgt gatgatgctg gtcagacact
    31201 gaccaccgat tgggtacaat ggaaccgtct ggacgaagac gagcagtatc gtggtcgtta
    31261 tcaaatctct gttaagccgc agggttatca gcaggcggtt acggttaaac tgctgaacct
    31321 ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca gcacggagat
    31381 gatgaacgtt atttccgccg gtctggataa atctgccact gacgccgcga acgctgcgca
    31441 aaatcgtgcc tccaccacta tggacgtaca aagtgcagct gatgacaccg gtttaccaat
    31501 gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt ctgccagcgg cgctggaaaa
    31561 agtgggcatg aaagtgaccg acagcacccg ttcgcagggc aacatggccg taacttataa
    31621 gccgctgtct gacagcgact ggcaggaact gggcgcgagc gatccaggcc tggcatccgg
    31681 tgactataaa ctgcaggttg gcgatttaga taaccgcagc agcctacagt tcatcgatcc
    31741 gaaaggtcat actctgactc agagtcagaa cgacgcgctg gtagctgtct tccaggctgc
    31801 gtttagcaag taaaaataca gggctggaat catccggccc ttttttctga tatgatacgc
    31861 aaacgtgtgc gtcggcagga aaacgcgatt ttagcggtaa ttcgcacgaa atttgtttgt
    31921 cggacgtagt tcggataagg cgttcacgcc gcatccgaca aaacatccgg cacaccagac
    31981 agcaaaagat tttaaaacgt taattcacac ccaggagtga taaagatgca aaagcaagct
    32041 gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga cctgttggtg
    32101 ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc gcattgagca gtttgatcgc
    32161 aaaggtatgg tgaacaacaa gttcaactac ttcattatga gcaaactggc tgaagcgggt
    32221 atcccgactc aaatggagcg tctgctctcc gataccgaat gtctggtgaa aaagctggat
    32281 atggtgccgg ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt gaaacgtctt
    32341 ggaatcgaag aaggtattga gctgaacccg ccgctgttcg atctgttcct gaaaaacgat
    32401 gccatgcacg atccgatggt caacgaatct tactgcgaaa cctttggctg ggtgagcaaa
    32461 gagaacctgg cgcgtatgaa agagctgacc tacaaagcga acgacgtgct gaaaaaactg
    32521 ttcgatgatg ctggtctgat tctggtcgac ttcaagctgg agtttggtct gtacaaaggt
    32581 gaagtggtac tgggcgatga gttctccccg gacggcagcc gcctgtggga caaagaaaca
    32641 ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg gtggcctgat cgaagcctat
    32701 gaagccgtcg cccgccgcct gggtgtacag ctggactgat ttttctggtt gcttgtttct
    32761 ttagccggag ggtactccgg ctaaatgtct ttgatacccc cgctacaata cttctcatta
    32821 tctccctttg acaggaaagt cataccaggc caatatgtat atacataacg tatatcccaa
    32881 aaaggggggg ctatggaaac tactgtattt ttaagtaatc gcagtcaggc cgtcagatta
    32941 ccgaaagcgg tcgctctgcc agaagacgtt aagaaagtag aaattattgc gattggtcgt
    33001 acaagaatca tcactcccgc cggtgagtcc tgggacagtt ggtttgatgg cgaaaatgtg
    33061 agtgcggatt ttatggatat ccgtgatcaa cccgcaatgc aggaaaggga atcattttga
    33121 tgctgaaatt catgctggat accaatatct gtattttcac tattaagaat aaaccagtac
    33181 atgtcagaga gcggtttaac ctgaattctg gccggatgtg tataagttct gtaacattga
    33241 tggagcttat ctatggggca gaaaaaagtc agatgccaga acgcaatttg gcggttattg
    33301 aaggtttcgt atctcgtttg gtagtgcttg attatgatag tgctgctgcg acgcataccg
    33361 ggcaaatcag ggccgagctt gcccggcagg gaaggcctgt tggacctttt gaccagatga
    33421 tagcgggaca tgctcgcagt cgcggtttga ttgtggttac caacaatacc cgagagtttg
    33481 agcgagtggc gggaatcaga ctagaagact ggagctaaca ttctctttta cttctaacat
    33541 tcgtaaatta tataacgtgg catgaagcct taattcctcc gccatttcat cccgctttcc
    33601 tgtggtaatc ttcctctgaa ccacctacga tcatttctat aatgaatata ttgttgaggt
    33661 atctatgcgt tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc gcaacagctc
    33721 cggtggtccg tctatgggcg gtcccggttt tcgcctgcca agcggtaaag gcgggctgat
    33781 tttactgata gtcgtgctgg ttgcaggcta ctatggtgtt gatttaaccg ggttaatgac
    33841 tggacagccg gtttcccaac aacaatcaac tcggtccatt agccctaatg aagacgaagc
    33901 tgcaaaattc acctcggtga ttctggcaac cacggaagac acctggggac aacagttcga
    33961 gaagatgggt aagacctatc agcaaccgaa gctggtcatg taccgtggaa tgacgcgcac
    34021 cggctgcggg gctggtcagt ccattatggg gccgttctat tgcccggcgg atggcacagt
    34081 ttatatcgat ctctccttct atgatgacat gaaagacaaa cttggcgcgg atggcgattt
    34141 tgcccagggg tacgttatcg cccatgaagt cggtcatcat gtgcagaaac tgttaggcat
    34201 cgagccgaaa gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtga accgcttatc
    34261 tgtgcgtatg gaactccagg ccgactgttt tgccggtgtc tgggggcata acatgcagca
    34321 gcaaggcgtt ctggaaaccg gcgatctgga agaggcgctg aatgcggcgc aggccatcgg
    34381 cgatgaccgt ttacaacagc aaagtcaggg gcgagtagta ccagacagtt tcacacatgg
    34441 cacttctcag caacgctaca gctggtttaa acgtggtttc gacagcggcg atccggcaca
    34501 atgcaatact tttggtaaaa gcatttaatt tccggggcag ggatggctga actgactgcg
    34561 ctttacacat taacagcgca aatgaaacgt gaagggatcc gccgcttgct ggtgttgagc
    34621 ggggaagagg gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt acctggcgac
    34681 tggctgtgga tttcgccgca gccagatgct gaaaaccact gttcaccctc ggcgctacaa
    34741 actttacttg ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg ctttgatgcc
    34801 gctgcctttg cagcacttag cggaacgttg aaagcgggaa gctggctggt tttgttactc
    34861 cctgtatggg aagagtggga aaaccaacct gatgccgact cgctgcgctg gagtgattgc
    34921 cctgacccta ttgcgacgcc gcattttgtc cagcatctca aacgcgtact tacggcggat
    34981 aacgacgcta tcctctggcg gcaaaaccag ccgttctcgt tggcgcattt tactccccgt
    35041 actgactggc accccgcgac cggcgcacca caaccagaac aacagcaact cttacagcag
    35101 ctactgacca tgcctccggg cgtggcagcg gtaacggctg cgcgtgggcg cggtaagtcg
    35161 gcgttggcag ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac cgcgcccgca
    35221 aaagcggcaa cggatgtact ggcacaattt gcgggcgaga agtttcgctt tattgcaccg
    35281 gatgccttgt tagccagcga tgagcaagcc gactggctgg tggtcgacga agccgcagcc
    35341 atacctgcgc cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt gttaaccact
    35401 acggtgcagg gctacgaagg caccggacgt ggttttttgc tgaaattttg cgctcgcttt
    35461 ccgcatttac accgttttga actgcaacag ccgatccgct gggcacaggg atgcccgctg
    35521 gaaaaaatgg ttagtgaggc actggttttt gacgatgaaa acttcaccca tacaccacaa
    35581 ggcaatatcg tcatttccgc atttgaacaa acgttatggc gaagcgagcc agaaacgccg
    35641 ttaaaggttt atcagttatt gtctggtgcg cactaccgga cttcgccgct ggatttacgc
    35701 cggatgatgg atgcaccagg gcaacatttt ttacaggcgg ctggcgaaaa cgagattgcc
    35761 ggagcgctgt ggctggtgga tgagggggga ttatctcaac aactcagtca ggcggtatgg
    35821 gcaggttttc gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc gcacggtagc
    35881 aatccactgg cagcgacatt gcgtggacgg cgggtcagcc ggatagcagt tcatccggcg
    35941 cgtcagcggg aaggcacagg gcagcaactt attgctggtg ctttgcaata tacgcgtgac
    36001 ctcgactatc tttcggtgag ttttggttac accggggagt tatggcgttt ctggcaccgc
    36061 tgcggttttg tgctggtgcg gatgggtaat catcgtgaag ccagcagcgg ttgctatacg
    36121 gcgatggcgc tgttaccgat gagtaatgcg ggtaaacaac tggctgaacg tgagcattac
    36181 cgtttacgtc gcgatgcgca agctctcgca aagtggaatg gcgaaacgct tcctgttgat
    36241 ccactaaacg atgccgtcct ttctgacgac gactggcttg aactggccgg ttttgctttc
    36301 gctcatcgtc cgctattaac atcgttaggt tgcttattgc gtctgctaca aaccagtgaa
    36361 ctggcattac cggcgttgcg tgggcgttta cagaaaaacg tcagcgacgc gcagttatgt
    36421 accacactta aactttcagg ccgcaagatg ttactggtcc gtcagcggga agaggccgcg
    36481 caggcgctgt tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg cataacgcaa
    36541 tggcaatttt ttcactgact ccttcaattt tctcccatgg tcattgtgag tcagcggcgt
    36601 aaaattaatg acatcagtta aggagttcgc catgaaacat gaccattttg ttgttcaaag
    36661 cccggataaa cctgcgcaac agttgttact gcttttccac ggtgtcgggg ataacccggt
    36721 ggcaatgggg gaaataggta gctggtttgc accgctgttt cctgatgcgc tggtggtgag
    36781 tgtcggtggc gcggagccga gcggtaatcc ggcggggcgt cagtggtttt cggtgcaggg
    36841 tattacggaa gataatcgcc aggcgcgtgt ggatgcgatc atgccgacgt ttattgaaac
    36901 ggtgcgctac tggcagaaac agagcggagt aggggctaac gccacggcgc tcatcggttt
    36961 ctctcaaggc gcgattatgg tgctggagag cattaaagcc gaaccgggcc ttgcttcacg
    37021 cgtcattgct tttaacggac ggtatgcgag cctgccggaa actgcgtcta cagccacgac
    37081 aattcacctg attcatggcg gtgaagatcc ggttatcgat ctggcgcatg cggttgcggc
    37141 gcaggaagcg ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg aggatttagg
    37201 ccatgcgatc gacaatcgca gtatgcaatt tgccctggat cacttgcgtt ataccattcc
    37261 gaaacattat ttcgacgaag cgctaagtgg tggtaagcct ggcgatgatg atgtgattga
    37321 aatgatgtaa aagacagatt acctggcctg ataagacgcg tgagcgtcgc atcaggcatc
    37381 gttcaccatt gccggatgcg gcgttaacgc cttatccggc ctacggttga gcactcagct
    37441 tacttcttcg gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa
    37501 tcacgatgcg ggggaagctc cggcttgttg gcgagaaatt tcttatggtc gacgcgcttg
    37561 agatctttaa tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa
    37621 tatttagcca gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc
    37681 tgttccatga tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat
    37741 tcattaattt tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg
    37801 cgagcaataa agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca
    37861 ttatagtgct caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat
    37921 ggctgcccgg aaagccacca gtccaccgta taacgcagtt gatgtttttc aagcagggca
    37981 agcacctgtg ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc
    38041 acaaacagtt cacccggaat aacgttgtta ctgcccgttc ccgcctgaat attagcaatc
    38101 tgcatactgg tcgccgggaa aaattcattg ccctgatccc actcaatagc cactaattca
    38161 ttaaggaaag gggctgcgcg atgtactgga ttgtcagcca gatgcgggta ggccacatgc
    38221 ccctgaacgc catgaatggt aaggttgcag gttaacgatc cgcgacgacc atttttcacc
    38281 acatcaccaa ccacttcgat actcgacggt tcgccgacca ggcagtaatc gagacgctca
    38341 ttacgcgcca ttaacgcttc gacgactttt accgttccgt tgtgggcact agcttcttca
    38401 tcagaggtga tcagaaatgc cagtcgcccc gtatggttgg gatgttgtgc gacaaaacgt
    38461 tctgccgcta ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat
    38521 aacatgccgt cacgaatggt tggttcaaac ggcggattga tccaacgatc ggcgtcgcca
    38581 ggcggcacca cgtcggtatg cccggcaaag gccaatgttt caccttgccc acgccatgcc
    38641 caaaaattct gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa
    38701 cgttcaatca acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata
    38761 agctgttgtg tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc
    38821 tgataactgg aatcactgaa acccagcagc ataggcttac cgggcgcgca gagcaatgga
    38881 cgtttgataa ttgcaggcat ttcagtcatt aatgccgccg cagaggccgc atcggtgatt
    38941 ttattgcggg tggtttcgtc cagtttacgc caggttgtac cacgggtgtt gagtaacgct
    39001 tcccagccta attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg
    39061 taatcatgaa aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg
    39121 gtgtcacaat ttttgatgcc gtaaagtgta accataatga atccttttt
//
LOCUS       DAZFAH010000023        38102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFAH010000023 DAZFAH010000000
VERSION     DAZFAH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.023"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHJWB_001112"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHJWB_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4312783.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="ACHJWB_001113"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="ACHJWB_001113"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4312784.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="ACHJWB_001114"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="ACHJWB_001114"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4312785.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(4357..4491)
                     /locus_tag="ACHJWB_001115"
     CDS             complement(4357..4491)
                     /locus_tag="ACHJWB_001115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312786.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="ACHJWB_001116"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="ACHJWB_001116"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4312787.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="ACHJWB_001117"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="ACHJWB_001117"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4312788.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGSEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="ACHJWB_001118"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="ACHJWB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4312789.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /gene="yijF"
                     /locus_tag="ACHJWB_001119"
     CDS             complement(8992..9609)
                     /gene="yijF"
                     /locus_tag="ACHJWB_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4312790.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="ACHJWB_001120"
     CDS             complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="ACHJWB_001120"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4312791.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="ACHJWB_001121"
     CDS             complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="ACHJWB_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4312792.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="ACHJWB_001122"
     CDS             complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="ACHJWB_001122"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4312793.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            14482..15561
                     /gene="frwC"
                     /locus_tag="ACHJWB_001123"
     CDS             14482..15561
                     /gene="frwC"
                     /locus_tag="ACHJWB_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4312794.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15576..15896
                     /gene="frwB"
                     /locus_tag="ACHJWB_001124"
     CDS             15576..15896
                     /gene="frwB"
                     /locus_tag="ACHJWB_001124"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4312795.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            15947..18244
                     /gene="pflD"
                     /locus_tag="ACHJWB_001125"
     CDS             15947..18244
                     /gene="pflD"
                     /locus_tag="ACHJWB_001125"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4312796.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            18210..19088
                     /gene="pflC"
                     /locus_tag="ACHJWB_001126"
     CDS             18210..19088
                     /gene="pflC"
                     /locus_tag="ACHJWB_001126"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO4312797.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            19090..19431
                     /gene="frwD"
                     /locus_tag="ACHJWB_001127"
     CDS             19090..19431
                     /gene="frwD"
                     /locus_tag="ACHJWB_001127"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4312798.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19418..20269)
                     /gene="yijO"
                     /locus_tag="ACHJWB_001128"
     CDS             complement(19418..20269)
                     /gene="yijO"
                     /locus_tag="ACHJWB_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4312799.1"
                     /translation="MYHDVSYLLSRLITGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLISHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(20418..22151)
                     /gene="eptC"
                     /locus_tag="ACHJWB_001129"
     CDS             complement(20418..22151)
                     /gene="eptC"
                     /locus_tag="ACHJWB_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4312800.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(22334..24985)
                     /gene="ppc"
                     /locus_tag="ACHJWB_001130"
     CDS             complement(22334..24985)
                     /gene="ppc"
                     /locus_tag="ACHJWB_001130"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4312801.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(25337..26488)
                     /gene="argE"
                     /locus_tag="ACHJWB_001131"
     CDS             complement(25337..26488)
                     /gene="argE"
                     /locus_tag="ACHJWB_001131"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4312802.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            26642..27646
                     /gene="argC"
                     /locus_tag="ACHJWB_001132"
     CDS             26642..27646
                     /gene="argC"
                     /locus_tag="ACHJWB_001132"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4312803.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            27654..28430
                     /gene="argB"
                     /locus_tag="ACHJWB_001133"
     CDS             27654..28430
                     /gene="argB"
                     /locus_tag="ACHJWB_001133"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4312804.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            28491..29864
                     /gene="argH"
                     /locus_tag="ACHJWB_001134"
     CDS             28491..29864
                     /gene="argH"
                     /locus_tag="ACHJWB_001134"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4312805.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            30131..31048
                     /gene="oxyR"
                     /locus_tag="ACHJWB_001135"
     CDS             30131..31048
                     /gene="oxyR"
                     /locus_tag="ACHJWB_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4312806.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(31031..32431)
                     /gene="sthA"
                     /locus_tag="ACHJWB_001136"
     CDS             complement(31031..32431)
                     /gene="sthA"
                     /locus_tag="ACHJWB_001136"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4312807.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            32762..33412
                     /gene="fabR"
                     /locus_tag="ACHJWB_001137"
     CDS             32762..33412
                     /gene="fabR"
                     /locus_tag="ACHJWB_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4312808.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            33412..33771
                     /gene="yijD"
                     /locus_tag="ACHJWB_001138"
     CDS             33412..33771
                     /gene="yijD"
                     /locus_tag="ACHJWB_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4312809.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(33811..34911)
                     /gene="trmA"
                     /locus_tag="ACHJWB_001139"
     CDS             complement(33811..34911)
                     /gene="trmA"
                     /locus_tag="ACHJWB_001139"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4312810.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      35025..35215
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            35280..37124
                     /gene="btuB"
                     /locus_tag="ACHJWB_001140"
     CDS             35280..37124
                     /gene="btuB"
                     /locus_tag="ACHJWB_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4312811.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTHDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AIIDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            37069..37926
                     /gene="murI"
                     /locus_tag="ACHJWB_001141"
     CDS             37069..37926
                     /gene="murI"
                     /locus_tag="ACHJWB_001141"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4312812.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRIKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         8651 a         9616 c        10556 g         9279 t
ORIGIN      
        1 attccactcc gcgccgctca tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca caatttatgt gggtactgac cgtaaacccg
      421 catagtttac cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg gcgtgataat
      481 gcatataatt ttaacggcta tttaggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaacacaat gtttactctg gtgcctgaca tttcaccgac
      601 aaagcccagg gaacttcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccg atccatcttt ccagcacata
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg gcaacccaac
      781 gcgcgatgtg gttcagcgtg cgctggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aatgaccgtc tttttgaaac ctggcgatct
      901 gctggttgcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 actggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttgggtgccg atctggtgtt
     1201 gcattcatgt acgaaatatc tgaacggtca ctctgacgtg gtggctggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgtatgga
     1381 gctggcccag cgcaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgccagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgggc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatttcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtgggccacg gggaagtatg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgtg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gccagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaac
     4021 ggcaaagcgc gtgtgggcgt ggaggcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccgtgcgata acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc
     4141 cgtgggcctg gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtt
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatta agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aacggataga tgtgcacagc acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ttgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagcca gaaatgtatg cttctgacct ggtgacgcta
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgcttaat ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt cttcgatgtc gaaagctacc tgcgttttcg tgaccgctgt
     5161 gtatcggcgg gcattgatgt ggaaattatt ccgggaattt tgccggtatc taactttaaa
     5221 caggcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcgcaaatg
     5281 ttcgacggcc tggatgatga tgccgaaacc cgcaaactgg tgggcgcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acacagtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgggaa aataaggttg tcagccttgt tttctccctc attacttgaa
     5581 ggatatgaag ctaaaaccct tttttataaa gcatttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgacctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcag gtacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcaggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaacgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggctgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 tctgttcaag tatgagtggg tacagacccg cagtccggct ggagcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac
     6901 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcttttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggtcg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct
     7621 ggtgggcggg atgcgcgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg accgacgaat cgaaagagct gtttgaaggc cgtgaccgcg aaaccggcga
     7801 agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtggctgaa gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     8041 actacacact ggatctctca tgtctgtcgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgtct
     8581 gtatgcccgt catccgcgcg tggatttatt gtcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccaa tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt agagaaaatg tccagaaggg gataaaaaac tcctgaacaa cgtaggagtt
     9721 tcagattact ggcacagagt gccggatgcg gcgtgaacgc cttatctggc ctaaaaagca
     9781 cgcaaattca atatattgca gagattgcgt agatactgat aagcgtagcg catcaggcaa
     9841 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     9901 ggaaacgctg accgtactgg tcggctacca gcagagcggc gtaaacctga tctggcgtcg
     9961 cgccgccagg catgttgtgg atggtttcac cttctgcaca tgccgcttct gccacaattc
    10021 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
    10081 ccgcatggct aagtgcagct acggtttcga tttcctcaac cggcgcgttt tccagtacca
    10141 gctgcgtcag cgtaccgaat gccacttttt caccgtgata atagtgatgc gcgtccggga
    10201 tagcggtcag gccgttatgc accgcatgag ccgccgccag tccgccgctt tcaaaaccaa
    10261 cgccgcttaa ataggtgttc gcttcaatca cgcgttccag cgcaggggtt actacatgct
    10321 gttcggcggc aagcatcgct ttttcgcctt cttccagcag agtgttgtag cacagttctg
    10381 ccagtgccag cgccgcctgg gtgcacttgc cgcccgccat agtggtagcg ccgctacggg
    10441 aacaggcgcg tgcttcaaac caggttgcca gcgcatcgcc gatacccgcc gctaacagac
    10501 gtgcaggagc gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca
    10561 acagtagata gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg
    10621 gtgcatcggt agaggcgata gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca
    10681 gtgctttggc agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg
    10741 cagtctccgc gatgccacgc agacgatcga tctcattttg cgaacattca ccgccaaacg
    10801 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa
    10861 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
    10921 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc
    10981 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtgttcg
    11041 aacttctcta tagctgactc taccgcaggg gtgttgagca tttgttgcgc tacatctaag
    11101 ggcagggtga tggattcaca tcctgccagt aaacagtcca gcgcctgacg cggcgtttta
    11161 aagctggccg ccagcaccat gctttctggt gcgtgcattt ctaacagcgc ttgcagctcc
    11221 tgaaccgtac gaatgccgtc cccgccctgg gcatctacgc ggttaacata cggagcaacg
    11281 tattttgccc ctgccagcgc ggccagtaac ccttgtgcag cactgtacac tgcggtgcca
    11341 agcgtagtaa taccctcttt tttcagcatc ttaatggcgg ccagaccttc ggaagtcacc
    11401 gggattttca ccacaatgcc cggaatggcg tcgcgcaggc gcttcgcttc ttccaccata
    11461 ccctgcgcgt cacgactcat agtctgagca aacagaatgc cctcatcacc aatcgctttt
    11521 tgcagacgcg gcagaacttc ccatatggac tccttgctgg cagcgataat gctcgggtta
    11581 gttgtcaccc cggcaatggg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    11641 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    11701 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    11761 tgcggcgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca
    11821 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac
    11881 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac
    11941 taaagcccag accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac
    12001 gcggatagcg gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    12061 ctttgctgcg ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct
    12121 gttgggtcat gcggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    12181 ttgcctggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    12241 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg
    12301 gcagcacgga acctttcgct cccagctcac cgcacagacc aatccatttg ccctggcgat
    12361 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt
    12421 gacgagtaac cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac
    12481 taccaatact aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg
    12541 gcacttccag cataatgccg agctggatct tctcatcaaa cggaatgtgt tcgttacgta
    12601 gttgctgttt ggcttctgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    12661 tcatcgggat catgattttc aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt
    12721 gtgtggtaaa taacgacgcg tattcttcat aaatacgcac ggcgcgataa ccgaggaacg
    12781 ggtttgcctc tgcgggaatg tttagataat caacgggttt gtcgccgcca atgtccatag
    12841 tgcgcacaat aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca
    12901 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac
    12961 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc tacggagtga gcgatgttag
    13021 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    13081 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt
    13141 ctcctggctc aaccacaatc gccccagcgt tgccgtcgat ataaatcgtt tgatgctgcc
    13201 acggagtaag ggcatcaata tctacaccaa ccagtgtcgg aatgttgaac gaacgggcaa
    13261 gaattacggt atgtgaggtg gtgccgccac ttttcagtaa cagacctttg aggtgatttt
    13321 tatccagttc gaggaactgg ctgggagtca gttcatcagc catacaaatg gcgggctgcg
    13381 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    13441 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    13501 cttcgcaaaa gtgattcgca ctggtgacaa tcgcttcggc gcagcttaat ccggcgctga
    13561 cgcctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgcgcttcca
    13621 gaatcgcgct ggtggcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca
    13681 gcgtcaggcc gttttccagt gcggattgct cggcgtcaac acctttggct gcgggaagat
    13741 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc
    13801 tgcacacggt gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    13861 gttccagttc gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc
    13921 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    13981 ggcagttatc gcccgccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    14041 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    14101 tttcaacgtg gcttgccgga cgcgcatgta cgccgttagg tagctcacaa ataaattcca
    14161 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    14221 gcaacggcct gccatgtgac aaatctgcca aaagctggac aaatgtaatg taaccgtcaa
    14281 tttgcgacgc gtctcacaag acgccgtttt gcggcatgct tccggattag cgcaagttat
    14341 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    14401 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    14461 ttgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    14521 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    14581 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccgaat
    14641 ttgaaaaaac tgtttgatat cggcgttgca ggtttgacgc tgatggtgcc tttcctcgcc
    14701 gcttacattg gttactccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    14761 tgggttggta acagctttgg tgcgggcttc tttggtgcgc tgatcgccgg gattatcggc
    14821 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    14881 cccatcttca ttattcctat catcggcaca ctgattaccg caggcatcat gatgtggggc
    14941 ttgggcgagc ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag
    15001 cagggcagca ttgttatgct ggcggtgatc atgggtctga tgctggcgtt cgatatgggc
    15061 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    15121 accgtggtgg ccatcgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg
    15181 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg
    15241 gtgatgggct gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    15301 cgtgtcattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    15361 ggtgcgcagt gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag
    15421 ctgggttata tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    15481 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    15541 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    15601 catgtccttc aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga
    15661 aagcgaaagg ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac
    15721 tgactgcgga agatgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca
    15781 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa
    15841 aacgtgctga cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc
    15901 ttctcccgct cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    15961 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgtt
    16021 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    16081 agcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    16141 cggtaaccgc accgtaaaac cgcgcgctgg gattatgtcg ccggaaatgg acccttactg
    16201 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagtga
    16261 agaagataaa cgtatctacc gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    16321 agatttcatc aacgggcaga tgacagatga agtaaaagcc gcgaccagca cgcagatttt
    16381 cagcatcaac cagacagata aaggccaggg gcacattatt attgattacc cacgcctgct
    16441 gaatcacggg ctgggggagc tggtggcgca gatgcagcaa cattgtcagc aacagccgga
    16501 gaatcacttt tatcaggccg cgctgttact gctggaagcc tcgcagaaac acattctgcg
    16561 ttacgctgaa ctggcggaaa caatggcggc aaactgcaca gatgcccagc gtcgcgaaga
    16621 actgctgact attgcggaga tctcccgcca taacgccgaa cataagccgc agacgttctg
    16681 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    16741 ttcgctatcg ttggggcgct tcgaccagta tatgttgccg ttctatcagg catctttaac
    16801 ccagggcgaa gatccggcgt tcctgaaaga actactcgaa tctttatggg tgaaatgcaa
    16861 cgacatcgtg ctgttgcgct ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg
    16921 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    16981 gttcctttgc cttgacgctt atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    17041 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg
    17101 caccggtatt ccgcaaatct ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg
    17161 cgtttcgctg gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    17221 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    17281 ctgcctgcat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    17341 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat
    17401 cggccatcgc gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga
    17461 aaaaggtcgc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    17521 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    17581 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg
    17641 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    17701 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    17761 gaacccgcgc ggcggttact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    17821 gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg
    17881 cggcttgtca cctatgctgg gccaggacgc acaagggcca acagcggtac tgaagtcagt
    17941 cagtaagctc gataatacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    18001 ggcgaccctg gaaggtgaag cgggattacg caaactggcc gacttcttac gggcgtttac
    18061 ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    18121 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    18181 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    18241 gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt
    18301 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    18361 gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg
    18421 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggggcgt
    18481 ttgaacggat tggccgcgat atcagccttg acgcgctgga acgggaagtg atgaaagatg
    18541 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    18601 aggcggagtt tgctacccgt tttttacagc ggctgcggtt gtggggcgtg tcatgcgcca
    18661 ttgaaactgc cggagacgcg ccagccagca agctattacc actggcgaaa ttgtgcgatg
    18721 aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    18781 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc
    18841 cgcgtttacc gttgatccct ggtttcacgc tcagccggga gaatatgcag caggcgctgg
    18901 atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg
    18961 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    19021 cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    19081 gaggttaaaa tggcatactt ggtggcagta accgcctgcg taagcggcgt ggcgcatact
    19141 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    19201 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg
    19261 gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc
    19321 cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg
    19381 gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    19441 ctgtcagttg gctgtggtac tgccggcgat attccgacgg cgagcgttcg gtgtttttac
    19501 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    19561 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    19621 attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagagatag tttggtgaaa
    19681 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    19741 aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt tggctgccga
    19801 gcaaatcgcg gcagtggcta atcaggctgg ctacgataag ccttgccgtt tgctgctcct
    19861 gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctata cgtgggccgc
    19921 gtcgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    19981 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt ggtagcgggt gttttccatt
    20041 gtgggaaatt ccagccacca gcggcaacgt acagcacatc gccgggaact aacgttgctc
    20101 cagcgccggt atcaacaaac tcaccttcca gcacaatttc cagccgtgga aaatcgacct
    20161 gatacgcaag atcgggcacc gggccgttac tgctggcaaa gtaaatctgg cgcagggaca
    20221 gcgggccggt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    20281 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    20341 gcctgagggg agttcgatgt aaacattgcg gcagtatcgc atccggcaaa tagcgccaga
    20401 cgcgatacaa aggtcattta ctgattaccc acctgatcgc catacggcag agtgtcgtaa
    20461 tcgatcagcg cgtttttctt gtacgggtta ccaatccagc gggtagtttc tttgaactgc
    20521 ggattcacca ctgaacgggt tggatcgtaa ccgtcgtaag ataagcccgc caaatctgac
    20581 caggtgtgga tcagttccgc caggctgtat ttacgatcga catcctgtga gaaatcacgg
    20641 ggatgagtcg cttgccattt ttccgacgtc cacagcagga acggaatggt gtacatgtgg
    20701 cgcgtcgggt tgtcttcgtt acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct
    20761 tcaccgtggt cagagaagta aaccaggaat ccgttcgggt ctgccgcttt aaagtcttta
    20821 atcaggctgg caaccacatg atcgttatac aggttagcgt tgtcataatc gttatatgat
    20881 tccagctctt ctgcgcttaa tcctggcgga acatgatcgg tattgccatc aaacttgccc
    20941 tgattttccg ggtagcggta tttgtatttg atatgcgtac ccagcagatg aacgatgatc
    21001 agtttcttcg gcgcagggtc attcagcaca tcctggaacg gcttcagcac gttggtgtcg
    21061 tattcacgcg cactctgcgt acgttgctgg ttcatgtagt actgcttgtc ggtctggcgc
    21121 gaaaataccg tcagcatggt attgcgggcg gtcatcgtct gctggttggt gatccagaag
    21181 gttttataac ccgcctgttt catcatgttc atcagcgacg gctgcgtcag atacagatcc
    21241 gggttctttt cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt gtacggacga
    21301 gacgtaacta cgttattaaa cacggtcaga ttcggatcgg ttttatgcag cgcatccagc
    21361 tccggcgtgg tttcacgcgg ataaccgtac agactcatgc gtccgcgctg ggtcgactcg
    21421 ccaatcacca gcactaaagt gcgcggttcg ttacccgatt catctttgaa attagccagt
    21481 ggcggcaagg cattattttc attcagtaac tttgttagcg agtttagttg ctgacgatac
    21541 tgataatagc cggtcaggaa ttgccacggt gcggcaggct ccatacgcga ggccaggtta
    21601 tccaacgttt tctcaaacgg cttgtttttg ataaacgtat tcatggcgat cggatgcaga
    21661 atcaagccat aaagcagggc aaaagagaca acataacgcc acggctttgg aatatagacc
    21721 gggcgcaggc gtgtccacag cagaactgcc accgccgtat aggccagcgc gataagcaca
    21781 attttcaggc tgaaatactg gcttaaatac tcgctggctt cgttggtgtt ggtttcgaac
    21841 atcacaaaca gaacgctctg cgagaactcc tgcccgtaga tgacgtagta gcatagcgcc
    21901 gccagagagg ccgcccatag caccacgccg attactgcgg caataatttt aatccgcttc
    21961 ggaaagagga ataccgggat caaccacagc gagctgaata acagcgagtc gcgaatgccg
    22021 ttggtgccac tataaccact gatgtaaata atggcctgta gcagagtaga gaaaaaccaa
    22081 aagtagagca gtgcccaacc cagggctttc cagctaaaaa ggggtttagc ctggacttct
    22141 gtggaatgca tagtaagaac ctgtcttgaa aaaaatatcg ccgaatgtaa cgacaattcc
    22201 ttaaggatat ctgaaggtat attcagaatt tgaataaaat gcagacagaa atatattgaa
    22261 aacgagggtg ttagaacaga agtatttcag aaaaccctcg cgcaaaagca cgagggtttg
    22321 cagaagagga agattagccg gtattacgca tacctgccgc aatcccggca atagtgacca
    22381 ttaacgcttg ttcgacgcga ggatccggtt cctggccttc tttttctgcc tggcgggagc
    22441 ggtgcagcaa ctcggcctgc aatacgttca gcgggtcggt gtaaatattc cgtagctgaa
    22501 tagactctgc aatccacggc agatcggcca tcagatggga atcgttggca atcgccagca
    22561 ccactttgat gtcttcttct tgcaggttgc gtaactcttt acctaacggc cacagtgctt
    22621 tgtctaccag acgttgatca tagtattccg ccagccacag gtctgctttg gcgaagacca
    22681 tctccagcat gccgagacgc gtcgagaaga atggccaatc gcggcacatg gcttccagct
    22741 cgctctgttt gccatcttcg accacttttt gcagcgccgt acctgcaccc agccaggcgg
    22801 ggagcatcag gcggttttgc gtccaggcaa aaatccacgg tatggcgcgc agcgactcga
    22861 cgccgccggt tgggcgacgt ttcgccggac gtgaacccaa cggcagtttg cccaattctt
    22921 gttccggcgt agcggagcgg aagtaaggca caaaatcttt gttttcacgt acgtagccac
    22981 ggtagagatc gcaggagatg actgacagtt catccataat gcgacgccag ctctctttcg
    23041 gctcaggtgg tggcagcagg ttggcttcca gaatcgcccc ggtgtacagc gacaggctgc
    23101 tgacggtgat ttctggcaga ccatatttaa agcggatcat ctcgccctgt tcagtcacgc
    23161 gcaggccacc tttcaggctt cctggcggtt gtgacagcag cgccgcatga gcaggtgcgc
    23221 cgccgcgacc aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata cccgcttttt
    23281 cgcaggtttt gattaatgca tcctgtgcct gatattgcgc ccaggaagct gccatcactc
    23341 ccgcatcttt tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc
    23401 cacgatacca gtcaatattg agcagctggg tcatgacatc gttggcgttg ttcagatcat
    23461 cgagggtttc aaacagcgga gcaaccggca tcgcaaaccc gatacccgct tctttcagca
    23521 gcaggtggac agccagtacg tcggacggcg ttttcgccat cgagatcacg taggcggcaa
    23581 tggagccttg cggcgcttcg gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg
    23641 cgcttggttg ccagttgcgc ggcagaagcg gacgtttgga gttcagttcg cggatcagga
    23701 acgcctgttt gtcggcctct gaccagcttt cgtagtcgcc gataccgagg tagcgggtca
    23761 gctcgcccag cgcttcggta tggcgggtgc tctcctgacg gatatcaata cggaccagcg
    23821 gtacgccgaa acatttcacg cggcgcaggg tgtcgagcag atcgccgttg gcgataatac
    23881 ccatgccaca cgcctgaagt gactggtagc aagcgtagag cggttcccac agttcttcgt
    23941 tttgtgtcag cagcccttct ggttttggca gttcttcgcc tttcaggcgc gcttccagcc
    24001 atgcctgtgt cgccatcagg cgagaacgca ggtttttcat cagatagcga tacggttctg
    24061 cggcaccttc ttcgccaacc agcgccagca gttcaggggt cgcttcaacc atcgacagtt
    24121 cagaaaccag cacctgaata tctttcagga acaaatcggt ggctttccag cggctgagta
    24181 gcaggacgtg gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg tcgccgccca
    24241 tccacgaagt aaaacggacc ggaacaaatt cgacgggcag tttgtagccg aggttctctt
    24301 ccagttgttc gttcagttcg cgcaggtaat ttggtacgcc ttgccacagg ctgttttcca
    24361 ctacggcaaa gccccatttg gcttcatcta ccgggcttgg acgcagctta cggatttcat
    24421 cggtatgcca tgactgggcg attaactggc gcaggcgacg catcagctgg ttgtgttcgt
    24481 agtcagcgat atctttgtta tcgagctgtt ttaagcaggc gttcacttcc accattttgt
    24541 ggatcagtgt acgacgggta atttcggtag ggtgagccgt gaggaccagt tccagcgaca
    24601 gcgattccac tgcttttttg atggtgtctt cgctcagttc cggctggttt ttcagtttac
    24661 gcagggtgcg ggcgatcact tccgggttgc tggcagcttc gcctttcggc gaaatgctgt
    24721 ggtattgctc ggcggtgttg gccaggttca ggaactgact aaacgcacgc gcaacgggca
    24781 gcagctcgtc gttcgacaaa ttttgtaagg tggtgagtaa ctcctggcgg ttagcatcat
    24841 tgccagcgcg tgaagatttc gacaacttac ggatagtttc tacgcgttca agaatgtgtt
    24901 ctcccaacgc atccttgatg gtttctccca gcactttgcc gagcatactg acattactac
    24961 gcaatgcgga atattgttcg ttcatattac cccagacacc ccatcttatc gtttgatagc
    25021 cctgtatcct tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt
    25081 tataaagcca cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata
    25141 aatagcagga atttacgtca ttaaattcac gacgctttaa ataagcgtaa cttatggaaa
    25201 tgttaaaaaa tcgccccaag taacaccaaa ggtgtaggtc ggataagatg cgcaagtatc
    25261 gcatccgaca ttattgcggc actggagttt gacaacagtg ccggatgcgg cgcgagcgcc
    25321 ttatccggcc tacgttttaa tgccagcaaa aatggtgaat tacctggata atcaactcac
    25381 gggtgggttt aataaaccgc gtctccagat attcatcagg ctgatgagct tgattgatgg
    25441 aacccggacc gagcaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt
    25501 aattcaccac ttcggttttt gctccgagca atttctcaac cacttcaacc agttgatgat
    25561 tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg
    25621 gccagcgttc gctcaccgga gccaatgcat cgttgagcaa accattaagt tcattgagtg
    25681 tcatgccagg cagcggacga atatccatat gcaactcaca gcaagcgcaa atacggttag
    25741 aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg gtatggcacg gtaaatgctt
    25801 cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt
    25861 gcattagttc gatagcgtta actccgcgtg ctggatcgct ggagtgcccc gactggccct
    25921 gaatacggat ggcgttagag atatgacctt tatgtgcgcg taccggttgt agtgacgtcg
    25981 gttcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc
    26041 cggccatact ggtttcttca tcagcagtcg ccagaatgta gagcggtttt ttcagtttcg
    26101 tgacgtcgac atcgcgtagc gtatcaagga taaacgcaaa aaagcctttc atgtcggcgg
    26161 tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatca cgcgtccagc
    26221 gaccgtcatc aaatggtacc gtatcggtat gccccgccag caacaagccg ccagccccct
    26281 gtccgcaact ggccagcata ttaaatttgt tgcgagttcc tggcacaggc tgcacttcca
    26341 cattgaagcc taaatcttta aaccagtccg ccagcagagt gattaaatct gcattgcttt
    26401 gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct
    26461 cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa
    26521 tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag
    26581 ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc
    26641 gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta
    26701 tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga
    26761 tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg agctgccgtt
    26821 gcagccgatg tcggatatca gcgagtttag cccaggggtg gacgtggtgt ttctcgccac
    26881 cgcccatgaa gttagccacg atttagcgcc gcagtttctt gaagcgggct gcgtggtgtt
    26941 cgacctttcc ggcgcgtttc gtgttaacga cgccaccttc tatgaaaaat attacggctt
    27001 tacccatcaa tacccggaac tgttggaaca ggcagcctac ggtctggcgg agtggtgcgg
    27061 taataaatta aaagaagcga atttgattgc ggtgccgggc tgttatccga cggcggcaca
    27121 gctggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat
    27181 caacgccacc agcggcgtga gcggtgcagg gcgtaaagcg gccatttcaa acagcttttg
    27241 tgaagttagc ctgcaaccgt atggcgtctt tactcatcgc catcaaccag agatcgccac
    27301 acacctcggt gctgacgtta tcttcacccc acatctgggc aatttcccgc gcggcattct
    27361 cgaaaccatt acctgccgcc tgaaatcggg tgtgacccag gcgcaagtcg cgcaagtgtt
    27421 acagcaggcg tatgcccata aaccgctggt gcggctgtat gacaaaggcg ttccggcgct
    27481 gaaaaatgtc gttgggctgc cattttgcga tatcgggttt gccgttcagg gcgagcatct
    27541 gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtacagtg
    27601 cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga
    27661 atccattaat tatcaaactg ggcggcgtac tgctggatag tgaagaggcg ctggaacgtc
    27721 tgtttagcgc actggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtgcacg
    27781 gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa
    27841 acggcctgcg ggtgacgcct gctgatcaga tagacattat caccggagca ctggcgggaa
    27901 cggcaaataa aaccctgttg gcatgggcga agaaacatca gattgcggcc gtaggtttgt
    27961 ttctcggtga cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggtcatgttg
    28021 gactggcgca gccaggttcg cctaagctta tcaactcctt gctggagaac ggttatctgc
    28081 cggtggtcag ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgacc
    28141 aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca
    28201 gcggcattct cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg aaagcagaac
    28261 aactgattga gcagggcatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg
    28321 atgcggcccg cacgctgggc cgtccggtag atatcgcctc ctggcgtcat gcggagcagc
    28381 ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg
    28441 ccggaggcgc agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt
    28501 ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc
    28561 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc
    28621 tggtcacggt aggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg
    28681 tgctgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata
    28741 tccatagctg ggtggaaggc aaactgatcg acaaagtggg ccagttaggc aaaaagctgc
    28801 ataccgggcg tagtcgtaac gatcaggtag cgactgacct gaaactgtgg tgcaaagata
    28861 ccgttagcga gttactgacg gctaaccgac agctgcaatc ggcgctggtg gaaaccgcac
    28921 aaaacaatca ggacgcggtc atgccaggtt acactcacct gcaacgcgcc cagccggtga
    28981 cgttcgcgca ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa agccgtttgc
    29041 aggatgcgct taagcgtctg gatgtcagcc cattaggctg tggcgcgctg gcgggaacgg
    29101 cctatgaaat cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta
    29161 acagtctcga cagcgtttct gaccgtgacc atgtgttgga actgctttct gctgccgcta
    29221 tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag
    29281 cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg ccgcagaaga
    29341 aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggggcgttaa
    29401 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag
    29461 acaaagaagg tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc
    29521 tggtgctgga cggcattcag gtgaaacgtc cacgttgcca ggaagcggct cagcagggtt
    29581 acgccaacgc caccgaactg gcggattatc tggtggcgaa aggcgtaccg ttccgcgagg
    29641 cgcaccatat tgttggtgaa gcggtggtgg aagccattcg tcagggcaaa ccgctggaag
    29701 atctgccgct cgacgagttg cagaaattca gcccggtgat tgacgaagat gtctatccga
    29761 ttctgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc tcaccgcagc
    29821 aggtggcgca ggcaattgct tttgcgcagg cgcggttggg gtaagaacat ttatatgtat
    29881 aaatttgagc ctggcttatc gccgggcttt tttatggcaa aaaaaagcgg atcctggaga
    29941 tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga aacgggcagt
    30001 gacttcaagg gttaaaagag gtgccgctcc gtttctgtga gcaattatca gtcagaatgc
    30061 ttgataggga taatcgttca ttgctattct acctatcgcc atgaactatc gtggcaatgg
    30121 aggatggata atgaatattc gtgatcttga gtacctggtg gcattggccg aacaccgcca
    30181 ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg ggcaaattcg
    30241 taagctggaa gatgagttgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt
    30301 cacccaggcg ggaatgctgc tggtagatca ggcgcgtacc gtactgcgtg aggtgaaagt
    30361 ccttaaagag atggcaagcc agcagggcga gacgatgtcc gggccgctgc acattggttt
    30421 gattcccaca gttggaccgt acctgctacc gcatattatc cctatgctgc accagacctt
    30481 tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga
    30541 cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga
    30601 agtgccgttg tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa
    30661 ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg
    30721 tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg atgaagatac
    30781 acacttccgc gcgaccagcc tggagacgct gcgcaacatg gtggcggcag gtagcgggat
    30841 cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct
    30901 gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc
    30961 gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcacgaatgg atggccattt
    31021 cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc agctacccga taggcttccg
    31081 ccatggtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg ccgccacctt
    31141 tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt
    31201 gaatacccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg cccacgttca
    31261 tgccgacgat ttgtgcgcgt gccagatgtt taaactgggc gcggcccact tcatatggca
    31321 ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc gggatggtgt
    31381 aaatgccggt agggatatct tcaatcagat gcgcggtggc ttcgcctttt accagcgcct
    31441 gcgcggcaat gcgcccctgg tcataggccg ctgatgccag gctcggataa ccaatcacgt
    31501 cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc
    31561 cgcgactgtc agtttccagc ccaatgttct gtaacgccag cgaatcggta ttaccggtgc
    31621 gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc aaatgcatga
    31681 tcacaccatc gtcacagcct tcgatcttct cgtactcttc gttgtgacga atcactacgc
    31741 cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga aatgccagca
    31801 ggcgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag atcgacgcat
    31861 attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg tggtgcatgc
    31921 taagaattga gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct gttggatgat
    31981 atggacgaga gccgcaggca ataacaaatt tttcagcggt tagtgtttcg acgctgccgt
    32041 ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg agcgttcccc tgcaatattt
    32101 cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta atcacgttat
    32161 cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgggaatgg tcgctgtata
    32221 gtgggttttg attgaattct ataatgcggc tgacggcgtg acggagagct ttcgacggga
    32281 tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata actgcgacgc
    32341 gcgcaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg gaacctatta
    32401 ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct tatacataaa
    32461 agcaacagaa tggtaacatt ttatcgcggg taagccaatt gatccccgtc atttatctgg
    32521 ctatatcctg agcggccttt gctttgtctg tttcttactt ttaccctgac gttttattgg
    32581 atttttatcg acgatactct ccgtttaagc ggcaggtttc cgctgtacgt aaaagaaccg
    32641 gccaaagaat tgcagtaaat atgttttatt gcgttaccgt tcattcacaa tactggagca
    32701 atccagtatg ttcattctct ggtatagtgc cagcagtact tttggcaagg attcagacat
    32761 cgtgatgggc gtaagagcgc aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc
    32821 atttagccaa ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg
    32881 tgaagcgggc attgctccca cctcttttta tcggcatttc cgcgacgtag acgaactggg
    32941 tctgaccatg gttgatgaga gcggtttaat gctacgccaa ctcatgcgcc aggcgcgtca
    33001 gcgtatcgcc aaaggcggga gtgtgatccg cacctcggtc tccacattta tggagttcat
    33061 cggtaataat cctaacgcct tccggttatt attgcgggaa cgctccggca cctccgctgc
    33121 gtttcgtgcc gccgttgcgc gtgaaattca gcacttcatt gcggaacttg cggactatct
    33181 ggaactcgaa aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag caatggtgac
    33241 aattgtcttc agtgcgggtg ccgaggcgtt ggacgtcggc gtcgaacaac gtcggcaatt
    33301 agaagagcga ctggtactgc aactgcgaat gatttcgaaa ggggcttatt actggtatcg
    33361 ccgtgaacaa gagaaaaccg caattattcc gggaaatgtg aaggacgagt aatgaaacaa
    33421 gcaaatcaag atagaggtac gctgctgctg gcgttagttg ctggcttatc gattaatggt
    33481 actttcgcag cgctgtttag ctccattgtg ccattttctg tattcccgat tatttccctg
    33541 gtgctgacgg tttactgcct gcatcaacgt tatcttaatc gcactatgcc ggtaggcttg
    33601 ccgggtctgg cagctgcctg ttttattctc ggcgtactgc tgtacagcac ggtagttcgt
    33661 gcggaatatc cggatatcgg ctctaacttc ttcccggcag tactctccgt cattatggtg
    33721 ttctggattg gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta atcggtatta
    33781 tgccggatgc tggcgcatcc ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc
    33841 catatggtgc gtgtagggga actgatcaaa cagagccaga cgttcgacct tgtgcgtctg
    33901 gcttaatgtt tccagattct tgcataacgt ttccgggtta caggagatgt acaaaatacg
    33961 cggatacgcc tgcaccattt tctcggtttc actgtccaga ccgctgcgcg gtgggtcgac
    34021 aaaaatggtt tcgcactgat aactctttaa gtcgatccct tgcaggcggt taaactcgcg
    34081 cacaccattc atcgcctgag taaattcttc tgccgccata cgaataattt gtacgttatc
    34141 aatatggtta gctgcgatgt tgtattgcgc agcagcaacc gacggcttag cgatttcggt
    34201 ggctaatacc cgatcaaaat tgcgcgccag cgctaatgaa aagttaccgt tgccgcagta
    34261 cagctccagt aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt ccagcatctg
    34321 aatattcatc gccgcgttcg gctgggtaaa gctgttttct acctgacggt agatcatctc
    34381 tttccctgcg accggcagac gctcgtcgat gtaatcttga tccagctcga ttttggtttt
    34441 cgttgcccga ccaatcagat gcacattcag attctgcgcg cgcagtgcat cgcgcagggc
    34501 ctccgcttcc tgacgccact catcatccag cttcttatgg tatagcaggg aaaccaccgc
    34561 ctgattactc agcgtggtga ggtaatcaat ctggaacaac ttgtggcgca gaacgggatt
    34621 attacgcaca cccgcaatca tcgccgtcat caactggttg ataagttcac tggcggcggg
    34681 gaagctatcc acgcggatac ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc
    34741 atcgccatcg tgccagatgc ggaactccgc acgcatccgg taatgactga ccggcgagcg
    34801 aaacacttcc ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac gtaccacttt
    34861 ttcggctaac tgcgcttcat actgttctgt tggaaggtgt tcgggggtca tgatgtatcc
    34921 tgaaaaatta aagtacgcgg cgattgtagg gattgctcat cagatgtcca gatcttgatg
    34981 aattcctatt tgtgagctac gtctggacag taacttgtta caacctgtag catccacttg
    35041 ccggtcctgt gagttaatag ggaatccagt gcgaatctgg agctgacgcg cagcggtaag
    35101 gaaaggtgcg atgattgcgt tatgcggaca ctgccatccg gtgggaagtc atcatctctt
    35161 agtatcttag atacccctcc aagcccgaag acctgccggc caacgtcgca tctggttctc
    35221 atcatcgcgt aatattgatg aaacctgcgg catccttctt ctattgtgga tgctttacaa
    35281 tgattaaaaa agcttcgctg ctgacggcgt gttctgtcac agccttttcc gcttgggcac
    35341 aggataccag cccggatact ctcgtcgtta ctgctaaccg ttttgaacag ccgcgcagca
    35401 ctgtgcttgc accaaccacc gttgtgaccc gtcaggatat cgaccgctgg cagtcgacct
    35461 cggtcaatga tgtgctgcgc cgtcttccgg gcgtcgatat cacccaaaac ggcggttcag
    35521 gtcagctctc atctattttt attcgcggta caaatgccag tcatgtgttg gtgttaattg
    35581 atggcgtacg cctgaatctg gcgggggtga gtggttctgc cgaccttagc cagttcccta
    35641 ttgcgcttgt ccagcgtgtt gaatatatcc gtgggccacg ctccgccgtt tatggttccg
    35701 atgcaatagg tggggtggtg aatatcatca cgacgcacga tgaacccgga acggaaattt
    35761 cagcagggtg gggaagcaat agttatcaga actatgatgt ctctacgcag caacaactgg
    35821 gggataagac acgagtaacg ttgttgggcg attatgccca tactcatggt tatgatgttg
    35881 ttgcctatgg taataccgga acgcaagcgc agacagataa cgatggtttt ttaagtaaaa
    35941 cgctttatgg cgcgctggag cataacttta ctgatgcctg gagcggcttt gtgcgcggct
    36001 atggctatga taaccgtacc aattatgacg cgtattactc tccgggttca ccattggtcg
    36061 atacccgtaa actctatagt caaagttggg acgccgggct gcgatataac ggcgaactga
    36121 ttaaatcaca actcattacc agctatagcc atagcaaaga ttacaactac gatccccatt
    36181 atggtcgtta tgattcgtcg gcgacgctcg atgagatgaa gcaatacacc gtccagtggg
    36241 caaacaatgt catcgttggt cacggtagta ttggtgcggg tgtcgactgg cagaaacaga
    36301 ctacgacgcc gggtacaggt tatgttgagg atggatatga tcaacgtaat accggcatct
    36361 atctgaccgg gctgcaacaa gtcggcgatt ttacctttga aggcgcagca cgcagcgacg
    36421 ataactcaca gtttggtcgt catggaacct ggcaaaccag cgccggttgg gaattcatcg
    36481 aaggttatcg cttcattgct tcctacggga catcttataa ggcaccaaat ctggggcaac
    36541 tgtatggctt ctacggaaat ccgaatctgg acccggagaa aagcaaacag tgggaaggcg
    36601 cgtttgaagg cttaaccgct ggggtgaact ggcgtatttc cggatatcgt aacgatgtca
    36661 gtgacttgat cgattatgat gatcacaccc tgaaatatta caacgaaggg aaagcgcgga
    36721 ttaagggcgt cgaggcgacc gccaattttg ataccggacc actgacgcat actgtgagtt
    36781 atgattatgt cgatgcgcgc aatgcaatta tcgacacgcc gttgttacgc cgtgctaaac
    36841 agcaggtgaa ataccagctc gactggcagt tgtatgactt cgactggggt attacttatc
    36901 agtatttagg cactcgctat gataaggatt actcatctta tccttatcaa accgttaaaa
    36961 tgggcggtgt gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct catctgacag
    37021 ttcgtggtaa aatagccaac ctgttcgaca aagattatga gacagtctat ggctaccaaa
    37081 ctgcaggacg ggaatacacc ttgtctggca gctacacctt ctgaaccacg tcccaccgtg
    37141 ctggtgtttg actccggcgt cggtgggttg tcggtctatg acgagatccg gcatctctta
    37201 ccggatctcc attacattta tgctttcgat aacgtcgctt tcccgtatgg cgaaaaaagc
    37261 gaagcgttta ttgttgagcg agtggtggca attgtcaccg cggtgcaaga acgttatccc
    37321 cttgcgctgg ctgtggtcgc ttgcaacact gccagtaccg tttcacttcc tgcattacgc
    37381 gaaaagttcg acttcccggt tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg
    37441 acggcaaatg gcattgtcgg attactggca acccgcggaa cagttaaacg ttcttatact
    37501 catgagctga tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg ctcggcagag
    37561 atggttgagt tggctgaagc gaagctacat ggcgaagatg tttctctgga tgcactaaaa
    37621 cgtatcctac gcccgtggtt aagaataaaa gagccgccag ataccgttgt attgggttgc
    37681 acccatttcc ctctactaca agaagaactg ttacaagtgc tgccagaggg aacccggctg
    37741 gtggattctg gcgcagcgat tgctcgccga acggcctggt tgttagaaca tgaagccccg
    37801 gatgcaaaat ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc agaagctgaa
    37861 caattattgc ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta
    37921 ggctgatttg gttgaatgtt gcgcggtcag aaaattattt taaatttcct cttgtcaggc
    37981 cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
    38041 gtcagcgggg ttctcctgag aatctcaaca gagaaaagca aaaaaatgct tgactctgta
    38101 gc
//
LOCUS       DAZFAH010000024        37404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFAH010000024 DAZFAH010000000
VERSION     DAZFAH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.024"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHJWB_001142"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHJWB_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4312813.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHJWB_001143"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHJWB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4312814.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVNKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHJWB_001144"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHJWB_001144"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4312815.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHJWB_001145"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHJWB_001145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4312816.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHJWB_001146"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHJWB_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4312817.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVTKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHJWB_001147"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHJWB_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4312818.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHIAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHJWB_001148"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHJWB_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4312819.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTEKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALTDNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHJWB_001149"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHJWB_001149"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4312820.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHJWB_001150"
     CDS             complement(8482..8526)
                     /locus_tag="ACHJWB_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312821.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHJWB_001151"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHJWB_001151"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4312822.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHJWB_001152"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHJWB_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4312823.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHJWB_001153"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHJWB_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4312824.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10220..10609)
                     /gene="mutT"
                     /locus_tag="ACHJWB_001154"
     CDS             complement(10220..10609)
                     /gene="mutT"
                     /locus_tag="ACHJWB_001154"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4312825.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10669..13374)
                     /gene="secA"
                     /locus_tag="ACHJWB_001155"
     CDS             complement(10669..13374)
                     /gene="secA"
                     /locus_tag="ACHJWB_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4312826.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13436..13948)
                     /gene="secM"
                     /locus_tag="ACHJWB_001156"
     CDS             complement(13436..13948)
                     /gene="secM"
                     /locus_tag="ACHJWB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4312827.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="ACHJWB_001157"
     CDS             complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="ACHJWB_001157"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4312828.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="ACHJWB_001158"
     CDS             complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="ACHJWB_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4312829.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="ACHJWB_001159"
     CDS             complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="ACHJWB_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4312830.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="ACHJWB_001160"
     CDS             complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="ACHJWB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4312831.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPIL
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="ACHJWB_001161"
     CDS             complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="ACHJWB_001161"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4312832.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19413..20888)
                     /gene="murC"
                     /locus_tag="ACHJWB_001162"
     CDS             complement(19413..20888)
                     /gene="murC"
                     /locus_tag="ACHJWB_001162"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4312833.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLESVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20942..22009)
                     /gene="murG"
                     /locus_tag="ACHJWB_001163"
     CDS             complement(20942..22009)
                     /gene="murG"
                     /locus_tag="ACHJWB_001163"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4312834.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYTWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="ACHJWB_001164"
     CDS             complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="ACHJWB_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4312835.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23250..24566)
                     /gene="murD"
                     /locus_tag="ACHJWB_001165"
     CDS             complement(23250..24566)
                     /gene="murD"
                     /locus_tag="ACHJWB_001165"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4312836.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="ACHJWB_001166"
     CDS             complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="ACHJWB_001166"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4312837.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25645..27003)
                     /gene="murF"
                     /locus_tag="ACHJWB_001167"
     CDS             complement(25645..27003)
                     /gene="murF"
                     /locus_tag="ACHJWB_001167"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4312838.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27000..28487)
                     /gene="murE"
                     /locus_tag="ACHJWB_001168"
     CDS             complement(27000..28487)
                     /gene="murE"
                     /locus_tag="ACHJWB_001168"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4312839.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="ACHJWB_001169"
     CDS             complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="ACHJWB_001169"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4312840.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERIFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="ACHJWB_001170"
     CDS             complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="ACHJWB_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4312841.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="ACHJWB_001171"
     CDS             complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="ACHJWB_001171"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4312842.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="ACHJWB_001172"
     CDS             complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="ACHJWB_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4312843.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32621..33625)
                     /gene="cra"
                     /locus_tag="ACHJWB_001173"
     CDS             complement(32621..33625)
                     /gene="cra"
                     /locus_tag="ACHJWB_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4312844.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYERKAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33707..33793)
                     /locus_tag="ACHJWB_001174"
     CDS             complement(33707..33793)
                     /locus_tag="ACHJWB_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4312845.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33805..34296)
                     /gene="ilvN"
                     /locus_tag="ACHJWB_001175"
     CDS             complement(33805..34296)
                     /gene="ilvN"
                     /locus_tag="ACHJWB_001175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4312846.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34299..36023)
                     /gene="ilvI"
                     /locus_tag="ACHJWB_001176"
     CDS             complement(34299..36023)
                     /gene="ilvI"
                     /locus_tag="ACHJWB_001176"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4312847.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36142..36186
                     /locus_tag="ACHJWB_001177"
     CDS             36142..36186
                     /locus_tag="ACHJWB_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312848.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36341..37285)
                     /gene="leuO"
                     /locus_tag="ACHJWB_001178"
     CDS             complement(36341..37285)
                     /gene="leuO"
                     /locus_tag="ACHJWB_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4312849.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9015 a        10428 c         9138 g         8823 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgc aacaattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gtaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca accaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac cgggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa atccccacct
      601 gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag
      661 tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga
      721 tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg agcgttagca
      781 acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg gcgttgtgcg
     1261 tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg ctggtggtat
     1321 ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgccc tcggcgtctc caccttattg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa
     1801 gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagtagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc acacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattactg gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacccggcct
     3601 gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaata
     4021 gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaagctgct
     4141 ttcccgtttc gttttttgcc cctcgcttgt gctgatggcg tttgccaaag tagcgccaac
     4201 caaatcaatg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc tgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt gacaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacgccgggt tgggataacg
     4561 caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac tgccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagagg
     4741 tggttcatat tgcggtagtc gatgcacctt cgcatgagct actggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctgactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgggtcag gcactggatg tcaacacgct tggaatgcag ccgttacaac
     5281 tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 ccggcagcgg caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacagcagag atcgctatta
     5581 aagcggcgca aactggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgacgg cagcaagggg
     5761 agcccatcca cattccagac aatgtatggc catcgccgct gccccactgg caggcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
     5881 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc gtagagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgta ctttactgct tatggcacta cagcaacaga tggttacccc actaagcctg
     6181 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctactcaa agcagggtta acgctttctg aagggctggc tctgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
     6361 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaaaagccca gcgtcagttg accgaaaaag tgaaatcagc gttgcgctat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcttt
     6721 ctgctggcga tagccaataa gttgctgatg cgccgcccta cctggcttat cgcgcggcag
     6781 aaattgttgt tacgaatccc gattatgggt tcactgatga ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgctaaaaa atgccggtga gtttagcccg
     7021 ctctgtttgc aattagtgag aacgggagag gcatccggct cgctggatct catgttagac
     7081 aacctcgccc atcatcatcg ggataacaca atggcgctga cggataacct cgcagcctta
     7141 ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacgcc agcgttggga gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cccgaataaa cgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caagccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc accgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccgcc aaaggctttc gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga
     7681 tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa agctgcggat
     7741 aaccgacgcc tgttttgaca cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcagc accggagagg ataagctcct cacacatttc accagtcact acgttgcccg
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccaca tctatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtacctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgacct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcaa cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgaa
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagtg aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgtgcgaatg tcggatgcgg
    10201 cgtgaacgcc ttatccgacc tacagacgct taagcttcgc aattaccggt tcattggctg
    10261 gcggaaaatc atcggcatta agaccgacca gcgacatcca ctcaccgggt tgcccttctt
    10321 taccccacgg caccccttcc caactttcga ccagccagaa ccacagagtt atatgcctgt
    10381 ccgggaattc atatgccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgacct
    10441 cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc gcccatttca attttaccgc
    10501 cgggaaactc cagtttattc gccatgtgcg catctgctgc gcgacgcgtt ataaagattt
    10561 cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt gttttaaacc
    10621 tataaaaaag gcgcagaatc ctgcgccttt tgcttcaaca gttagctttt attgcaggcg
    10681 gccatgacac tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac
    10741 tttgcgctca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    10801 gctaagctgc tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    10861 ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa cttcatattt
    10921 caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    10981 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgccgccag
    11041 gtgctctttc cacagagagt cgagagtttg cagcatgacg cctttttcga agtgacgcat
    11101 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    11161 tcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cggcaattgg
    11221 caaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    11281 ttccagcgac tgtggcggaa tgtaggcatc aatggtggct ttgaacacat cttcacgaat
    11341 gctgttgatg gtttcgctca catcgctgac atccagcagt tcgttacgct gggagtaaat
    11401 ggcgcggcgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    11461 gttacgactt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc
    11521 aatggcttcg cctggcttca tacccagttt acgcatcatt ccggatactc ggtcagaagc
    11581 aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc
    11641 ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt
    11701 accgatgata tgcaggccac ctgcttccag taccgcgtcg tgacgtacct gccagtcggc
    11761 tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca
    11821 gctaccaccg agcacgatat ctgtaccacg acccgccatg ttggtcgcga tagtcaccgc
    11881 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag
    11941 gacattgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat
    12001 ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    12061 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    12121 cattggacgg ttggtcggaa caacgacggt atccagtttg tagatggagc taaattcgaa
    12181 agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt
    12241 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    12301 ttccacagcc tggtgcagac catcggacca gcgacggccc tgcatggtac gaccggtgtg
    12361 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac gggtaaacag
    12421 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    12481 cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag
    12541 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    12601 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    12661 gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc
    12721 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    12781 acgttcttca gggctgaacg ccatgttgtc gcgcaggtag tcaaagccgt attcgttgtt
    12841 cgtaccgtaa gtgatgtcag ctgcataagc ttcacgcttt gccggtgctg gcatgcccgg
    12901 caggttaata ccaacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    12961 acgttgcgcc aggtagtcgt tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt
    13021 caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat
    13081 gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa
    13141 gacgcgctta cttgcctcac gtaccacggc gaaagcttcc gggatcagat tttccagcac
    13201 ttcgcctttt tccagacgcg cacgaaactc tgcggttttc cctttcagtt cttcgtcgga
    13261 gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    13321 cagggtgcga tcgttacgac taccgaaaac tttagttaat aatttgatta gcataataaa
    13381 atctcaaacg ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagc
    13441 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    13501 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    13561 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    13621 gcaaagattc ttcagcaacg ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga
    13681 ttaccgtgcg aatagcatgt tgatgccagt aatcaacgga atagttcgaa ttagggcggc
    13741 gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    13801 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac
    13861 ctaaactcgc cgcgaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    13921 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgaat tcaaacgtat
    13981 ttagcgcaca aatcttatca ttaaagcctg aactccacag cacgaatgaa gataagttgt
    14041 gcgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagctctt ataaaattca
    14101 aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt cgaattttat
    14161 acgacagtat aaatgtcgtt atgccagtac agctgaaggc gctttgaagg ccaacggcag
    14221 ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgtttcgcca ggacagcctg
    14281 cagcagtttg ttattcagtg catgaccgga tttataagcg gtaaatgcac caataatatt
    14341 gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtggcgca caaattcgtc
    14401 ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc
    14461 gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac gcatgaaacc
    14521 gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt tcatcgcata
    14581 gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa
    14641 accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa cagtctcttt
    14701 gatgcgaaca aatttcttgg cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta
    14761 tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa cttcgataac
    14821 aatgttatcg atgcccaggc ccgcgagagc agcattgagg tgctctacgg ttgaaatccg
    14881 tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag atttggcatc
    14941 ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtgttggc
    15001 cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga cccccgtcgc
    15061 ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc aaattaccta
    15121 tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc ctcgaaaccc
    15181 aaattccagt caattcttaa tcagcttgct tacgcaggaa tgctgggata tccagataat
    15241 ccggctcttt cgcagtttgc ggcgcattgt cattcacgac tttagcaacc ggcttctgct
    15301 cctgggtcag cggagccatc ccatgctgct ggtagcgatc catcactggc tgctgaacct
    15361 gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct gtcgcaacaa
    15421 cggttacgcg cagctcgtca ttcatatccg ggtcaagaga agtaccgata accacagtcg
    15481 cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca tccagacgca
    15541 ggtcgaagcc cgccgtgatg ttaaccagca cgccgcgcgc gccagacagg tcgatatctt
    15601 ccagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct tcaccgctcg
    15661 ccacgccaga acccatcatt gcgtagccca tttcagacat tacggtgcgt acgtctgcaa
    15721 agtccacgtt catcaaaccc ggacgagtaa tcagttcagc gataccttgc acagcgcctt
    15781 tcagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg cccagaactt
    15841 tcagcagttt gtcgttcggg atagtgatca gagagtccac atgcttggac agttcagtga
    15901 tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa gttgaaaggc ttagtgacga
    15961 cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactggt gctgcacctg
    16021 taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct tccagcgccg
    16081 cacgcagtgc atcgcgatcc tcatcagccg cattgcggcc aacttctgga ttagcgccag
    16141 cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc gctgttttac
    16201 gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaatg cgctcgcgca
    16261 ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt
    16321 cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct gtcgcctgag
    16381 gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa ctattgagtc
    16441 gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct tcaccgttaa
    16501 gatgtgactc tttcccatag tgaagcaatc ccaccgccgt cgaataatac ggctcctgag
    16561 cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca
    16621 cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccggtt aatacaatgc
    16681 ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac tgcaatatct
    16741 cttcgttgac caggttgagc agctcggtat agcgcggctc gatcacctct gccagggtct
    16801 gacgttgcag actccgcggc ggacgaccgc ctacgctcgg cacttccacg ctctcatctt
    16861 ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg gcgtcacttg
    16921 gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca taaggaatta
    16981 ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat atccattgta ccaccaccga
    17041 tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa taacttgatg
    17101 ccagcccagc aaatatcagt tggtcaactt tcagcccaca acgttcaacc gctttgacga
    17161 tgtttttcgc catatcgttg tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc
    17221 ccgaaagtcc taccggattc ttgatccctt cctgatagtc aatcgcatac tcttgcggga
    17281 tcacatgcag cacacgatgc tcatcgcgca cacgcaccga tttcgcggta tggacgacgt
    17341 tttccacatc ttcttgcgtc acttcttctt cagaaatagg caccatacca atttcattct
    17401 ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga caatctgcca
    17461 tcaattctgc ctggtcaatg gcgcgttgta cgcacttgac cacggattcg aggtcgttca
    17521 ccccgccttt atccatacca cgtgacgggc agctgcccac gccaatgata ttgaccatac
    17581 cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatc tccagtccta
    17641 ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct gattttgttg
    17701 ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag agtcataacg
    17761 caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta aaaccggata
    17821 aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat tgagcttaat
    17881 atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt
    17941 aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca cttcattggc
    18001 gctgccttcc gggccataca gtattggaag cacctgcttg ctggtgcgat ctggcggcac
    18061 gctgaaggtg tttccttccg cgtctaccat gtgttgatca ttccaccgcg caatcggcac
    18121 atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc tcacctgctt
    18181 aatccacggc aggcgttgtt ctatttgcgt ctggatgatg ttgacatcct gggtcataaa
    18241 ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac gcgtgtaatg
    18301 gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt ccatccagcc
    18361 caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga aaaggatccc
    18421 cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt
    18481 cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc aactgcgaga
    18541 agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc atacccggtg
    18601 aggtattggc ttccagcaga taaaactgtc catcgctgtc cagcataacg tcaatacgtc
    18661 cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc aaattggcct
    18721 cttgtgacgc ttccagacct gcggggcaga aatactgtgt ctcatcagag agatacttcg
    18781 cctcataatc atagaaggtt ccggacggtt gaatacgtat tgacggtaaa atttcttcac
    18841 cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat acttcttcat
    18901 cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact acttttgaca
    18961 ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaaccc agagcagaaa
    19021 tttctgctaa ctgcttatca ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc
    19081 acggagcgac cggtaaaccg gcaccttgcc acagaagttt gctgcgtagt ttatccattg
    19141 aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc
    19201 cctgcagcgt gccatcttca ccgccgcgac cgtgtagcgc gataaacact ttctgaaagc
    19261 ccatcgactt cagttgcgtc acgtcgactt ctttcgggtc gacaggatac gcgtcaatac
    19321 cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact tcccgctcag
    19381 cggaggtccc gcccaacagg accgcgattt tatcagtcat gttgttcttc ctccggagtt
    19441 tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccgatatt accagccccc
    19501 tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc tacctgcgcc
    19561 ggatccggca ccagaatggg atctattttc ccacgtccac gaattgtgcg acacagcgaa
    19621 cggctgtccg ctcccggaat tggcgcttcg ccagccggat acacttccag catcaacagg
    19681 gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa
    19741 cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcacg cgctgcttta
    19801 atagtggcgt ccacttccgt cgggtggtgg ccgtagtcat cgaccagcat cgccgtaccg
    19861 cttttaccat tcactgactc cagcgggaat tcaccgagga aatcaaaacg gcgaccagtc
    19921 ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt caatgccctc ttccgtagca
    19981 accgcaaccg cagctgcggc gttcagcgcg ttatgacgac ctggcgcatt cagggtgaca
    20041 cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga
    20101 taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc
    20161 acacgcggta acaattcgcg gatcacagga tcatcaacac acatcaccgc acgaccgtaa
    20221 aacggcaggt tgtgcagaaa attaataaaa gtctgtttta aattctcaaa gtcgccctgg
    20281 taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc
    20341 aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg ccccaaacgc
    20401 gcatgaaccc ccgccgcttt taccagcccg ccgttaacga aggttgggtc gagcccagct
    20461 tctgcgtaga tgctggaaac catcgcggtg gttgtcgttt tgccgtgcgt tccggcaatg
    20521 gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg gatcaccgga
    20581 atacgcgctt catgagctgc gacaatttcc gggttatcgg cagaaatcgc gctggaaaca
    20641 acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat cgtcgctccc
    20701 agattcatta actgttgcgt gaccggattt ggcgctaaat cggaaccact gatctgataa
    20761 ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccaat gccgacaaaa
    20821 tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc caattgttgt
    20881 gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg catcgctaca
    20941 attacgcccg ggcagcccgg ctcacttcat ttgccactcg ctcggtggca tccggaatgg
    21001 atgcggcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac ccggccaggg
    21061 tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct gcgcccgctt
    21121 tttccagcgg tagcgcattc cagtattgct ggcggtcttt atgttgaaac ggcacaaaca
    21181 acgccggtag tcctgccgcg gcgatttcac tcaccgttaa cgcaccggag cggcaaacga
    21241 cgacatccgc ccacgtatac gccgccgcca tatcatcaat aaattccgtc actttatgct
    21301 gcggttgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct ttgccgctct
    21361 gatgccagat agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa
    21421 gaatgcgtgc gccctgggaa ccacccacta ccagcacacg aaccggacct tcacgtccag
    21481 ccaaacgttg ctgcggcaac ggtagcgcca acacatcggt acgcaccggg ttacccacca
    21541 cttccgcatt agggaaagca cctggaaacg cctgcatcac tttggtggca atcttcgcca
    21601 gccatttatt ggttaagccc gcaataccgt tttgttcatg aagcacaacc ggaatgccta
    21661 acgaccacgc ggccagacca cctggacctg atacgtagcc gcccataccg agcaccacgt
    21721 caggtttgta cgctttcata atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg
    21781 gggcagctat cagtgctttt ataccttttc cacgcagacc agagatacga atgaaatcaa
    21841 tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc cccagccagc
    21901 gaacttgcca accctgagcc attagatggt gcgcaaccgc cagtcccggg aatacatgtc
    21961 caccggttcc gcctgccatc accattaatc gctttccttg accactcatc gtgaacctcg
    22021 tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca gcatcatgat
    22081 ggctgtcgac ataatcagta agctcgaacc accgtaactg attagcggca atgtcagacc
    22141 tttagtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga agctaaacca
    22201 gatgccaata gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta atgctttacg
    22261 gccaatcgac atcgcgcgaa aagcgacgaa gaataccatt aaaagtgcca gcaccacacc
    22321 gacatacccc agttcttcgc cgataatggc gaaaataaag tcagtgtgcg cttccggcag
    22381 atactccagt ttttgtaccg agttacctaa accttgcccc caaagttcgc cgcgaccaaa
    22441 cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc acgggttcca
    22501 gaatgcggta acacggcgga tacggtacgg ttcggcgagt atcagcaaca caaccgctga
    22561 aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca ggaacaacat
    22621 cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg ccagcagtaa
    22681 cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac
    22741 ttcgtcgcct ttacgcacca gatagttggc gatatagcaa aacagcgaca gttttgtcag
    22801 ctccgcaggc tggatacgca gcaaaccgag atcgatccaa cgcgatgccc ctttaacaga
    22861 gctacccact accagcacga tcatcagcag gatgatagat ccgagcagca tcgtggcact
    22921 gtagcgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa tgaatgccag
    22981 gatcagatag acgccatcac gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc
    23041 tatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcgaggc cgaaggtcag
    23101 ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc gcgagcccat
    23161 cacccagccc tttagcgccg tggagatcca gaccaggata ctgaatcctg gcaggcgcgg
    23221 cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag acgggcaaac
    23281 tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc cggggagagc
    23341 agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatcgcctg ttccatagtt
    23401 tcggtttgtt ctgccacttc cgggcgtagc gccgccaact gcgcgccgtc acgaccgaaa
    23461 caatacagac gtacgttatc gccattcagg taacgcgcca gtgggctaaa gtccgccgat
    23521 ttaccatcgc cacccagcaa caaatgcagc gtgccgtcta cgtgcaggcc attcagcgcc
    23581 gcttccgtac tgccgacgtt ggtcgctttt gaatcgttaa tccaacgtac gccgttatgc
    23641 tccagcacaa cttcaaagcg atgcggcaga ccggtgaatg tggttaacgc tttcaggctg
    23701 ctggcgcgcg gcaaaccagc tgcgtctgcc agtgccagtg ctgcgagcgc attggtgtag
    23761 ttatgctgcc cggaaagttt catctctttc acgttcagca ctttctcgcc tttcacccgc
    23821 agccaggttt cgccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg
    23881 acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc attaaccacg
    23941 caaactttcg cgttttcgta aatgcgcagt tttgctgcac gatactgttg taaaccaaac
    24001 ggatagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaag
    24061 ctggaggtgg tttccagctg gaagctcgac agttccagca cgtacagttc acactcatca
    24121 tccagtagca tcaacgcagg caggccaata ttgccaccca caccaacgtt aacccccgcc
    24181 gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc
    24241 gccacaatcg gtgcttgtgc ttcgcgacag aacagctcga tatcgccaac gatttcgatt
    24301 ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca
    24361 atcagatctg ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct
    24421 tcaggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca
    24481 ccgcgagcga ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgaca
    24541 acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc agaccaatca
    24601 ggaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt
    24661 tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa
    24721 aggagccgac ctgcaggatg acagaaagcg tttctaccac gaacacgccc cccataatca
    24781 ccagcaggaa ttcctgacgt agcagtacgg cgataatgcc taacgcacca cctaacgcca
    24841 gcgaaccgac atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca
    24901 gtcctgcccc gactatcgcg gtacagacaa taaccagttc cccggcgtgt cgcagatacg
    24961 gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac
    25021 caccggcgac aaatacggtc ggcataattg ccagaccgtc aagaccatcg gtcaggttta
    25081 ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt
    25141 gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccaa
    25201 caaggtacag cgcgaaggcg acacccagcg caatgaccga catccagaaa tacttccaac
    25261 gagcgatcaa ccctttggtg tctttacgca ctactttgcg ataatcatca acaaagccaa
    25321 taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag
    25381 cccacagcag tacggagatc acaatcgccg tcaggatcat aatcccgccc atggtcggcg
    25441 taccgcgctt gctgaagtgt gattcaggac cgtcgttacg caccacctga ccaaaggaaa
    25501 gtttttgcaa atgagcaatc atacgcgggc ccatccacaa tgagatgaac agcgcggtca
    25561 gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt
    25621 tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaatg cgcgtactac
    25681 ctcttccatg gcggcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc
    25741 aatcagtgat ttaagacgcg taattaacgc agttttatca gcaaaatgtt cgccaacgcc
    25801 gctggcagtg ctgatagcat ggctttgttt acccacgctt aacacgcggt caataccagc
    25861 agcttttgcc gcttcgccca cttgtacatg gcaggcttcg ctttcagcgc ccagttccgc
    25921 catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct ggactgctgc
    25981 ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag
    26041 ttggatgggg aacagacggc ctggaacagc tttcagattt gccagccccg ctttgatagc
    26101 atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt
    26161 gtgacgcccc ggcaacggca gcagaacatc cacgctaccg gttggggttt gcagggtaaa
    26221 ttccgtaccg tgcgaagtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt
    26281 cggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt
    26341 gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt
    26401 cgctttcgcg acccccgcaa gcgagccaaa accttccaga tgtgccgctg ccaggttgtt
    26461 gaccagcgcc gcttccgggc gagtcagact cacagtccag gctatttcgc cctgatggtt
    26521 cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg
    26581 tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa
    26641 aatcgccgcc gtcatctctt taacggaggt tttgccggag gaacctgtca gagcaaccac
    26701 gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc
    26761 cttgacgatt aactgcggca ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc
    26821 gccgccagct ttcgcctggt cggcaaaatc atgagcatcg aaacgttcgc ctttcagggc
    26881 aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat
    26941 atctgcacct tgcagttcac cgttgagaat gtcggtaagt tggctaaggg ttacgctaat
    27001 catgcaatca cccccagcag acgcgccact gtgacgcgat cggagtagtc cagacgctga
    27061 ttgccaacaa tctggtaatc ttcatggcct ttgcccgcga ccagcaccac atcgttctct
    27121 ttagcctgca taacggcgca agtcaccgct tcagcacggc cttccatcac tttggcatgt
    27181 ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggctc ttcggtacgc
    27241 gggttatcgt ccgtcaccac cgccacgtca gcaaactctt cggcaattgc gcccatcagt
    27301 ggacgcttac ctttatcgcg atcgccacca cagccaaaga cacaccacag cttgcccgca
    27361 cagtgcagac gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc
    27421 accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc
    27481 agacgcgcgg cggttttcaa cagatcagcc aatggatagc cgagtgccaa cagtgtcgcc
    27541 agcgcgagca gcaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca
    27601 tcgccccaac ttgagctaaa gcgaatcgtc gcaccgctgt cgtgatagtt cacgtcgatc
    27661 gcttttaacc agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaacc
    27721 gcgtccggca gttttgccag ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc
    27781 tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca
    27841 ccatgataat caaggtgatc gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat
    27901 gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaaagtcgcg
    27961 ccctgatcca ccagccccgc cagctcatgc tgaacatcga ctgcggaacc ggtagtattt
    28021 tctgtcggga tcactttccc aagcagaccg ttaccaacgg tgcccattac cgcgctggtt
    28081 tcgccaagca gttggctcca ctgcgccagc agctgggtag tcgtggtttt gccgttggtg
    28141 cccgttacgc ctacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt
    28201 gcagataaac gctcattgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg
    28261 atttcaccgt cggtcgcctc atctttcgcc tctgcaataa tggcagccac accttgcgct
    28321 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    28381 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    28441 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    28501 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    28561 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    28621 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    28681 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    28741 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    28801 gccgccgccc ggtagcgcca cgctttccat catatgcacc acggtgcgga caatggattc
    28861 cgggaagata cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    28921 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    28981 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    29041 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    29101 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    29161 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    29221 ttcgtggccg ttaattcgat aaggaacggt attgagtacc gagttttccc gcaccacgcc
    29281 acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    29341 ggtgatggta cggttacgca tcgcctcttt cggcgtgcca ctcagattgt tagggttgta
    29401 tgatgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    29461 accagattca gccttgttaa aggccaccgc attgttcagt tcgcgataaa ccagcgcctg
    29521 caggcgttca tcaatactca gcgccaggtt gtgcgcagcc tggctgtcag tagaagaaat
    29581 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    29641 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    29701 gccaatgagg tgagcagtca cttcgccgga tggataataa cggcgagatt cttcacgcag
    29761 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt ttacctgacg
    29821 cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag
    29881 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    29941 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    30001 gcgaccagaa cgatcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    30061 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    30121 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    30181 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    30241 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    30301 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    30361 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    30421 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa
    30481 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    30541 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    30601 ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg
    30661 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    30721 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    30781 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    30841 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    30901 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    30961 ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg
    31021 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    31081 aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt
    31141 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    31201 cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac accaagatcg
    31261 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    31321 gaaaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    31381 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    31441 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    31501 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    31561 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    31621 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    31681 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    31741 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    31801 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    31861 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    31921 ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg ataagcgccc
    31981 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    32041 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    32101 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    32161 ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa gaaaaaaaac
    32221 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    32281 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    32341 ctgtgtggcg cttgatgcca gcgggtttgc gccaaaacaa agaaaagtgt ttgttaaatc
    32401 ggcaattcat cgccgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    32461 tattgtgaat tttagctggt gagttaatta acgggcgggt ttaaaagcat ttatcaataa
    32521 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccagtcccgt actctacgcg
    32581 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
    32641 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
    32701 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
    32761 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
    32821 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
    32881 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
    32941 ataactgggc agccgccttc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
    33001 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
    33061 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
    33121 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa atgttcacga tcgagggcgc
    33181 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
    33241 gtggcaacga cgtcgaaaca ataatggcat caacctgacg ctgtaaaagg tgctcaatgc
    33301 agcgcatttc gttgtctggc tgatcttctg agcaggcaat cagcagttga taaccccgtt
    33361 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
    33421 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
    33481 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
    33541 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
    33601 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
    33661 attgtactgg tatgggttaa attcattaac atctaagcgc agaaaattaa ctgcggcaac
    33721 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
    33781 gcaaattgcg cactgagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
    33841 cacaccagag cgagcaactt ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc
    33901 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
    33961 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
    34021 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
    34081 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
    34141 gcccacggtc tggatggtca tacgcgataa tgtcggatcg tcggttggcg caacggtcag
    34201 gctttcaatg ttgtagcctc gctgggaaaa aaggccaatc acgcgggata acgcgcctga
    34261 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
    34321 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcactgcca
    34381 tcgacggtaa catcaacaaa caccaggcga ttattgcgca cctgttccag cgcctcgcta
    34441 agtttgcttt ccagctcatg cggatgagag atctggatcc cgacgtgacc ataggcttcc
    34501 gccagacgga cgaaatcggg tagtgattgc atataagatt gtgaatgacg gccggaatag
    34561 atcatgtcct gccactgttt caccatcccc agatagcggt tattgagatt caccaccagt
    34621 acgggcaact cgtattgcaa ggcggtagac agttcctgga tgttcatctg aatactgccg
    34681 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
    34741 ggtaaaccaa aacccatcgt gccgaggccg ccggaattga tccagcgacg cggtttgtcg
    34801 aatggataat aaagcgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
    34861 tccccattag tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
    34921 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatctgctg ccaccagtcg
    34981 cggatctcat ccagcggttg atgggcggat tcttgcgaca ggagttcaag catttgttcg
    35041 aggacctggc gagcatcccc cacaatcggg atatcagcag tcacggtttt agaaatggaa
    35101 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattattc
    35161 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
    35221 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
    35281 ggaaacgccc ccagccccat caatgagcaa acaacgggca gattcaacgc ctccaccgtt
    35341 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaactggt
    35401 ttttttgccg ctaccagcgt ttgcagagca cgcttaattt gccctttatg cccggtagta
    35461 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
    35521 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgctgcc
    35581 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
    35641 tgtttaacca ccggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
    35701 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
    35761 gtggcgatgc cggtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
    35821 acttccccgg tcgcacgcgc caggccatcg gccatatgca ccgccgcctg ctcatgacga
    35881 actaatacat gatcaatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
    35941 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
    36001 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
    36061 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
    36121 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
    36181 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
    36241 atcctaaaat agagagccca cacattcagg ataaaattct acattcaata aaaaatgcag
    36301 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
    36361 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
    36421 gataacaggt tctgctgttt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
    36481 cagcgaactc ttcagccaac caacgcggcg caatagcgac cagatgcgtt tgcgacacca
    36541 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
    36601 tgtcatacca aggttgacta aatgatgcga aacgatcaag cgaaaccgcc gcatgttgtt
    36661 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
    36721 ccagcaccat ttcatcttta aataatggta cgctggtaaa ttcaggacga tggaagtctt
    36781 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
    36841 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
    36901 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
    36961 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctggcgaa
    37021 ctgaaccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
    37081 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
    37141 acattcccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
    37201 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
    37261 gatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
    37321 ttattggaat gcagagaaat aatcctggag aatatgattt atcatgcata taaaataaga
    37381 aaaaggaaaa tgagtaaaat tagg
//
LOCUS       DAZFAH010000025        35596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFAH010000025 DAZFAH010000000
VERSION     DAZFAH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.025"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(186..917)
                     /gene="rlmB"
                     /locus_tag="ACHJWB_001179"
     CDS             complement(186..917)
                     /gene="rlmB"
                     /locus_tag="ACHJWB_001179"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4312850.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1097..3538)
                     /gene="rnr"
                     /locus_tag="ACHJWB_001180"
     CDS             complement(1097..3538)
                     /gene="rnr"
                     /locus_tag="ACHJWB_001180"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4312851.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3577..4002)
                     /gene="nsrR"
                     /locus_tag="ACHJWB_001181"
     CDS             complement(3577..4002)
                     /gene="nsrR"
                     /locus_tag="ACHJWB_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4312852.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4207..5505)
                     /gene="purA"
                     /locus_tag="ACHJWB_001182"
     CDS             complement(4207..5505)
                     /gene="purA"
                     /locus_tag="ACHJWB_001182"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4312853.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTEWAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5609..5807)
                     /gene="yjeT"
                     /locus_tag="ACHJWB_001183"
                     /pseudo
     CDS             complement(5609..5807)
                     /gene="yjeT"
                     /locus_tag="ACHJWB_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
     gene            complement(5889..6893)
                     /gene="hflC"
                     /locus_tag="ACHJWB_001184"
     CDS             complement(5889..6893)
                     /gene="hflC"
                     /locus_tag="ACHJWB_001184"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4312854.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6896..8155)
                     /gene="hflK"
                     /locus_tag="ACHJWB_001185"
     CDS             complement(6896..8155)
                     /gene="hflK"
                     /locus_tag="ACHJWB_001185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4312855.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8241..9521)
                     /gene="hflX"
                     /locus_tag="ACHJWB_001186"
     CDS             complement(8241..9521)
                     /gene="hflX"
                     /locus_tag="ACHJWB_001186"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4312856.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCDCRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9597..9905)
                     /gene="hfq"
                     /locus_tag="ACHJWB_001187"
     CDS             complement(9597..9905)
                     /gene="hfq"
                     /locus_tag="ACHJWB_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4312857.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9991..10941)
                     /gene="miaA"
                     /locus_tag="ACHJWB_001188"
     CDS             complement(9991..10941)
                     /gene="miaA"
                     /locus_tag="ACHJWB_001188"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4312858.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10934..12781)
                     /gene="mutL"
                     /locus_tag="ACHJWB_001189"
     CDS             complement(10934..12781)
                     /gene="mutL"
                     /locus_tag="ACHJWB_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4312859.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12791..14128)
                     /gene="amiB"
                     /locus_tag="ACHJWB_001190"
     CDS             complement(12791..14128)
                     /gene="amiB"
                     /locus_tag="ACHJWB_001190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4312860.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14147..14608)
                     /gene="tsaE"
                     /locus_tag="ACHJWB_001191"
     CDS             complement(14147..14608)
                     /gene="tsaE"
                     /locus_tag="ACHJWB_001191"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4312861.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14580..16127)
                     /gene="nnr"
                     /locus_tag="ACHJWB_001192"
     CDS             complement(14580..16127)
                     /gene="nnr"
                     /locus_tag="ACHJWB_001192"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4312862.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16126..17265
                     /gene="queG"
                     /locus_tag="ACHJWB_001193"
     CDS             16126..17265
                     /gene="queG"
                     /locus_tag="ACHJWB_001193"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4312863.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17248..17301)
                     /locus_tag="ACHJWB_001194"
     CDS             complement(17248..17301)
                     /locus_tag="ACHJWB_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4312864.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17535..17610)
                     /locus_tag="ACHJWB_001195"
     tRNA            complement(17535..17610)
                     /locus_tag="ACHJWB_001195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17575..17577),aa:Gly,seq:gcc)
     gene            complement(17646..17721)
                     /locus_tag="ACHJWB_001196"
     tRNA            complement(17646..17721)
                     /locus_tag="ACHJWB_001196"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17686..17688),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHJWB_001197"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHJWB_001197"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHJWB_001198"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHJWB_001198"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4312865.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHJWB_001199"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHJWB_001199"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4312866.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHJWB_001200"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHJWB_001200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4312867.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHJWB_001201"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHJWB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4312868.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLHGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24175..24489)
                     /gene="yjeO"
                     /locus_tag="ACHJWB_001202"
     CDS             complement(24175..24489)
                     /gene="yjeO"
                     /locus_tag="ACHJWB_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO4312869.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24486..24800)
                     /gene="yjeN"
                     /locus_tag="ACHJWB_001203"
     CDS             complement(24486..24800)
                     /gene="yjeN"
                     /locus_tag="ACHJWB_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO4312870.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLSDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24852..26354)
                     /gene="yjeM"
                     /locus_tag="ACHJWB_001204"
     CDS             complement(24852..26354)
                     /gene="yjeM"
                     /locus_tag="ACHJWB_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4312871.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26573..27550)
                     /gene="epmA"
                     /locus_tag="ACHJWB_001205"
     CDS             complement(26573..27550)
                     /gene="epmA"
                     /locus_tag="ACHJWB_001205"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4312872.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQMLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27875..29683
                     /gene="frdA"
                     /locus_tag="ACHJWB_001206"
     CDS             27875..29683
                     /gene="frdA"
                     /locus_tag="ACHJWB_001206"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4312873.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29676..30410
                     /gene="frdB"
                     /locus_tag="ACHJWB_001207"
     CDS             29676..30410
                     /gene="frdB"
                     /locus_tag="ACHJWB_001207"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4312874.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30421..30816
                     /gene="frdC"
                     /locus_tag="ACHJWB_001208"
     CDS             30421..30816
                     /gene="frdC"
                     /locus_tag="ACHJWB_001208"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4312875.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30827..31186
                     /gene="frdD"
                     /locus_tag="ACHJWB_001209"
     CDS             30827..31186
                     /gene="frdD"
                     /locus_tag="ACHJWB_001209"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4312876.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31249..32382
                     /gene="ampC"
                     /locus_tag="ACHJWB_001210"
     CDS             31249..32382
                     /gene="ampC"
                     /locus_tag="ACHJWB_001210"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4312877.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWKPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32471..33004
                     /gene="blc"
                     /locus_tag="ACHJWB_001211"
     CDS             32471..33004
                     /gene="blc"
                     /locus_tag="ACHJWB_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4312878.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVANNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(33001..33318)
                     /gene="sugE"
                     /locus_tag="ACHJWB_001212"
     CDS             complement(33001..33318)
                     /gene="sugE"
                     /locus_tag="ACHJWB_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4312879.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33500..33646)
                     /gene="ecnB"
                     /locus_tag="ACHJWB_001213"
     CDS             complement(33500..33646)
                     /gene="ecnB"
                     /locus_tag="ACHJWB_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4312880.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33757..33882)
                     /gene="ecnA"
                     /locus_tag="ACHJWB_001214"
     CDS             complement(33757..33882)
                     /gene="ecnA"
                     /locus_tag="ACHJWB_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4312881.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33934..34500)
                     /gene="efp"
                     /locus_tag="ACHJWB_001215"
     CDS             complement(33934..34500)
                     /gene="efp"
                     /locus_tag="ACHJWB_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4312882.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34542..35570
                     /gene="epmB"
                     /locus_tag="ACHJWB_001216"
     CDS             34542..35570
                     /gene="epmB"
                     /locus_tag="ACHJWB_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4312883.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8486 a         9767 c         9046 g         8297 t
ORIGIN      
        1 aatattttgt tcaggtacag tttgcagcgc cgtcgccagc gccaacggat tccatgtcat
       61 atattattcc atatagattt agtttaaata ttaataaaat gaatatttgc agtacgtaat
      121 tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt cataattcag
      181 acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc aaccgaaacg
      241 ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca atgttcacgg
      301 gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaggcg tccggtcatt
      361 ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca gatattctct
      421 tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg aacgctttct
      481 gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg atctttcggc
      541 acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc gaggttgtgc
      601 ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc gatcagatcc
      661 ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat gccctgatgc
      721 acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat aaccacgccc
      781 tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc ttttaaaata
      841 aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc gtggatgccg
      901 taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc actacgttta
      961 tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg ccggataagg
     1021 cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa ccctcttctt
     1081 ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc gctttggtcg
     1141 ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg tctttcttcg
     1201 ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca aagtttacct
     1261 ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt ttcgctttct
     1321 cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga ctaaagtcga
     1381 ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg cccaggcgat
     1441 aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag cgatagtagt
     1501 cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc agacggacga
     1561 agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct acctggtcga
     1621 gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg gcacgacgtt
     1681 ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa tggtagccgc
     1741 cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatat ttaatggcgc
     1801 ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca taggactgca
     1861 atgccagacc gaagtgacca cggttttctg gatcgtaaat cgcctgtttc atcgagcgta
     1921 gcagcatggt ttgcagcatt tctgcatcag gacggtcagc aaccgactcc agcagttccg
     1981 cgtagtcacg cggttccggc ttattaccac ccggcagctc cagccccagc tccgccagca
     2041 ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg
     2101 gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc atgcactctt
     2161 caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata cggcgttcag
     2221 cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt tcttcacggg
     2281 ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc agcggggcgt
     2341 actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg taggtcagac
     2401 gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg cctttcgacg
     2461 aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc gagcacaggc
     2521 cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac accgacgtac
     2581 cacgattacg cgcttctctg tccagcggcg ttggcggacg cacatagtag ctgacgtcgg
     2641 caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa actgcatcgt
     2701 caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc aaatcgacac
     2761 ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc ggcaacctgt tgctcaacag
     2821 cctgcggcca gatgtacgga atttcatggg tacgcagagc gatatcaacc gccatgccgg
     2881 tgcccatatt atcgccaagg acttcgacga ttttacccac cgctttggta cggcgagtcg
     2941 ggcgttgggt cagttcaaca accaccacaa agcccatccg cgcgcccatg atctgatcgg
     3001 gcgggattaa gatatcgaag ctcagacggc tgtcgtcagg aaccacaaag ccgacgcccg
     3061 cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg cggacaatac
     3121 gcgcttcacg acgaccttta cggtcagcgc ccagcggctg agccagcacc tgatcgccat
     3181 gaatgcaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc ccttcaaccc
     3241 gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg tcgaggcgtt
     3301 ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc tccatcgcgc
     3361 gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt tctaccgcca
     3421 gctcatcacg gctggccggt ttttcacgtt tagttaaatg ttcgaggata aattcccgac
     3481 tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct tgtgacatct
     3541 cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca ataatttata
     3601 aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa
     3661 actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa tgtggcaaaa
     3721 ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat caccaatacg
     3781 tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa cagcagtcac
     3841 gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg agacgccgta
     3901 gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg ccatgtagat
     3961 cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct caaaagggaa
     4021 atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt cgctaattta
     4081 tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag cgtaatggct
     4141 taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct gccaggcgta
     4201 ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat ccggaccggt
     4261 agagatgata tcgatcggca caccagtcag ctcttcaata cgcttgatgt agttcagcgc
     4321 cgcctgcggc agaccgctac gatctttcac gccgaaggtg gattcagacc agcccggcat
     4381 ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag tggtagtcac
     4441 ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca ggccatccag
     4501 aacgtccagt ttagtcaggc agaagccaga cagggagttc agctgtaccg cacgacgaac
     4561 ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc cgaattcgtt
     4621 accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga aaggacctgc
     4681 acctacacga gttgagtaag ctttgaggat acccagaacg taatcaacat aacgcgggcc
     4741 caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta cgtacggata
     4801 agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga cgaaatcgcc
     4861 acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag tcaggatgtc
     4921 ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag ctttgtagta
     4981 gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag cgaaggtttc
     5041 tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt cataagcagg
     5101 cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt
     5161 atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca gcagacgctc
     5221 acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg cggccggaga
     5281 cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac ctgatggaat
     5341 aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt tgtgaccgcc
     5401 ctggtagcgt acaacatatt tagcccattc agtcagaaga tcgacgatct tacctttacc
     5461 ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc aaaatcaccg
     5521 tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt ttgcactcaa
     5581 aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac atgtagtaga
     5641 ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta tcgggcaaat
     5701 tggtcatcgc agatgatcat cttcttccat gccttcgggt aaagcatcgg ccctaaacct
     5761 tccagtacca aaaccagggc aagcgccagc cagattgtcg aattcattca ttatccttat
     5821 agaaaaagaa aaccaccgac atccctgagg atgcggtggc tttattgacc tgtaccgcag
     5881 tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc
     5941 gggctcatga ccatcacgtc ctgattgcca gagaagctgt tctcataagc acgcaggcta
     6001 cggatgaatg cgtagaagtc cggatcttta ctgaatgcat cagcaaacag tttggctgct
     6061 tcggcatcgc cttcaccacg catgatgcgg ccctgacgct cagcttctgc cagcgttctg
     6121 gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg
     6181 tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt tgtagatcgc ttcagacact
     6241 tcggtcggca ggttgatctg cttgatacgc acatcgacaa cttcaatacc cagcgccgcc
     6301 atactgttcg ggttgatgac cggaactttg cccttcgtct cagccgttac gcgctctgcc
     6361 gcttcggcaa tggcgttatc tgctgccggg gtagtaactt catcttctgt acccgcagaa
     6421 ccggagttca gcgcgtcacg tacttcgagg gtcagacgac cacgggaatc agtgacgata
     6481 tctttcacgt ccaggcgacc aatttcagaa cgcagacggt cagagaactt acgtttcaac
     6541 agcacttccg cttgcgaaat gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg
     6601 ctgatgcgcc atttgatgta agaatcgacg atcaggtctt tcttctcttt ggtcacaaag
     6661 cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg
     6721 aacggtatct tgaaatgcag acccggctca taaaccagag gtttgttgtc atcgtcacgc
     6781 agtaccttac caaaacgcag cgtaataccg cgctcacctt ctttgacgac aaagacagac
     6841 atgtaaagca ctaccagcac gatgatgata atcgcgataa ctgacttacg catcgttatt
     6901 ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc
     6961 cctgactggt ggacgacgtg ttgcttgctc cactggttgt ggaggaagag gctggcggca
     7021 gacgcagcag attgctggca ccgttatcgc tcttcgccgc aggggcgtta ccacctttca
     7081 gcatctggtc taacggcaga accatcaggt tgccaccttt gtcgttaacc agcactttgc
     7141 gggtgttgcc caacactttt tccatcgtct cgatatacag acgctcgcga gtaatttccg
     7201 gcgcggcttt atattccggc agaagtttag caaagcgcgc cacttcaccc tgagcttcca
     7261 ggatggtctg ggccttgtac gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg
     7321 cacgcggctg aacttcgttg gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt
     7381 cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc ttccggcgga cgagcagcct
     7441 ggaagttgac gtccagcagc gtgataccca tgtcatacgg acgaatcgtc tcttccagtt
     7501 cgcgctgagt atcgctacga atcacggtac gaccttccgt cagaatgcgg tccatggtgt
     7561 atttaccgat aactccacgc agggcgctgt cggtagcctg acgcaggctg tcatccgggc
     7621 tggtcacgct atacagatat ttttccggat tggtgacgcg gtactgcacg ttcatctcaa
     7681 cgcgcactac gttctcgtcc gacgtcagca tcacaccaga agcggccagt tcacgcacgg
     7741 cttccacgtt caccggtttg acttcgtcga taaacgtcgg tttccagttc agacccggct
     7801 caaccagatg gctgaattta ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg
     7861 tatagaaacc actggccgcc cagataatga caatcgctgc cgctgcgatg gtaacgacac
     7921 gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc accgccagat ccggtgcctt
     7981 taccgccgcc cagaccaccg agctttttgc tcagtttgcg gaagatatca tctaaatcag
     8041 gtggcccttg atcgcgaccg cctttgtttc catttccctc agagttgccg ccaggtttgc
     8101 tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg ctgattccac gccatgtttg
     8161 tgctccatat ttgttatgcg gtgatccctg ttgtctttcg acgccagagg atatgactcc
     8221 acgcttcaga cgctacgccg ttagatcagg taatcgatca acgccggttc ttgtttacag
     8281 aggcgacgcc agtcaacgat cggcatacga acttgcagac ttacgctgcc gtcctcctcc
     8341 atccactctt tttctattgc ctgaagctga taaaaacgac ttctcagacg tccttcctgc
     8401 ggcggcaaac gcaatgtatg ctgcgccacc tcgccggaaa gccgctccgt caaagcctga
     8461 aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc agacacggat cggtttgttc
     8521 tcttcgtccc gatcaatacg cggttcgaaa tcttccagca tatcgatctt gttcatcacc
     8581 agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg
     8641 ttttcttgta cacgcacatc cgccgcatca atgacgtgca gcagtaatgt tgcttgccgc
     8701 gtctcttgta acgtggcttt aaatgccgcc accagatcgt gcggcaggtg gcgaataaac
     8761 cctacggtat ctgcaagtac ggtttcaccg acatctgcaa cgtcaatacg ccgcaacgtc
     8821 gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg
     8881 aaaagggtag atttaccggc gttggtatat cccaccagcg aaacagtagg aacgtcggct
     8941 ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa ctctttccag gcgcgactgt
     9001 atctgcacga tgcgattacg caacaaacga cggtcggttt cgagctgggt ttcacccgga
     9061 ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg tccagccacg caccaggcgc
     9121 gtagccagat ggcgcagctg cgccagctca acctgcaact taccctcatg ggtacgcgca
     9181 cgttgggcga aaatatctaa aataaggccg gtgcggtcga taacacgaca gtcgcacaaa
     9241 cgctccaggt tacgctcttg cgccgggctc agggcatggt caaaaagaac gaccgaagca
     9301 cccgtcgctt tgacagcttc cgcaatttca actgctttac cttcacctac aaaatacttt
     9361 gggtgcggcg ctttacggct accggtaatc acctgcaatg cttcgacacc ggcggaagag
     9421 accagagatt caaactcctg gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt
     9481 accagtaccg cctgctcacc agcatcataa cggtcaaaca agcgtataac cctctaaata
     9541 gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa aaaacagccc gaaaccttat
     9601 tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc atgatggtag
     9661 ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccggacg agacgggaca
     9721 acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa caggatcacg
     9781 aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa ataaatagaa
     9841 actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga ttgcccctta
     9901 gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat tctgaaaaaa
     9961 ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa caacctgtaa
    10021 tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc cttcccaacc
    10081 acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac
    10141 catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat aacccacgca
    10201 acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc
    10261 ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat ggagcagttc
    10321 acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt ctcctgacgt
    10381 ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg cccgggaaag
    10441 cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct cctgaagttg
    10501 acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg cccgcacttc
    10561 cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat acaacatcgt
    10621 accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt ccgccagcgc
    10681 atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg
    10741 gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat ccatcccttt
    10801 gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac gcagctcaat
    10861 ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct
    10921 cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa tcaacagatt
    10981 gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc acgtccgcca
    11041 gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga tttcgtgcaa
    11101 tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag cctatcagtt
    11161 caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc ctgatggtca
    11221 catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac tgcgcttttt
    11281 ctaatgccga tttttcttcg gcagaaactt ttagccgcaa cggaatcagc agcggctggg
    11341 cgcaaacggg cgcttcaccc ggcgtcaatt gtgcctgacg tagccaacgt tctgccactg
    11401 gcaaggataa aagtgaaatg ttgccatcgc gctccagcaa cgcacagtcg gaatggacga
    11461 tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg
    11521 gcgcttttgg tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt
    11581 gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga ctgcctgatg
    11641 ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct gcaaagtgat
    11701 tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg ggttcatcgt
    11761 ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc tgatagataa
    11821 aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg tgcacgttga
    11881 cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc tgatcggccc
    11941 ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg cgcatcatgc
    12001 gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga tttggatcgg
    12061 ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc gcttgttcaa
    12121 gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct tccggcactg
    12181 cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg tcgaaacgcg
    12241 ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc tcggtgcgca
    12301 ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc gtcgtcccca
    12361 ccggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca taggcctgcc
    12421 aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac
    12481 tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg tccagagagg
    12541 cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt ttgataccgc
    12601 agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca atatcgatac
    12661 gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac gcaggtcgct
    12721 cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc tgaattggca
    12781 tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg gcagtttgtg
    12841 ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg cgcaggcctt
    12901 tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc
    12961 tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc gaacgcagaa
    13021 cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt tgcaactgac
    13081 tgatcatact ggtcgctaca tcatacccta cccgttggga atgaccgaac tgtaaatcca
    13141 gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatca cccgccccgc
    13201 ccagcagctc cgactgtttc tcatgctgtt ccagccagct ggccatttcg ctgttggcgc
    13261 gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt gcggcatcag
    13321 cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcactg cgccccatca
    13381 ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga tcgtcattga
    13441 gcaaagtacg caatttacgc gcgatggcga tggtgacatt tttctcccgc gtaccaccgg
    13501 ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg ataattttat
    13561 cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg ctgataacac
    13621 ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg
    13681 cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggcggagga ggcggtggcg
    13741 gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc cgcttcaccg
    13801 cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt gcatctttag
    13861 gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac ggcagtccct
    13921 gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga tggctaaacg
    13981 cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa
    14041 cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc agcgtcgcta
    14101 ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa ccggctaaac
    14161 gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca cggccttgtg
    14221 cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta ccttgttgtg
    14281 gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc ataaactcca
    14341 gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa ttgtcgagcg
    14401 tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga cccagagctt
    14461 gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa atcgccatac agatagatta
    14521 cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt gcctgctcat
    14581 cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg gttaacaata
    14641 cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa acgcgccgcc
    14701 agtacgtcag ctgccgcacc gtgcgcgaca cagcctgcac aggctgcatc atacggcgac
    14761 agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat gccgccgctc
    14821 gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc ggcgaccacg
    14881 gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg tttggcgcaa
    14941 tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc ggcctcgcca
    15001 ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag caggttcaat
    15061 gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag ggcttttttc
    15121 ccccactctt gctggcccag accgggacca atcaccacca catcggccca ttccaggctt
    15181 tcggtaagag agtccatagt cagttcatgc accatcaatt ctggtcgtgc agtcagcagc
    15241 ggcgcaatgt tctcactgcg ggtcagtact cggactaaac cagcaccagc acgtagcgcc
    15301 gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat aattaccagc
    15361 cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt ttagccagtg agaaagttgt
    15421 tctgctgaaa accgctgaat tttcgtctcc tgacctgcca gccaactatc cagccccagt
    15481 gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc cagtgagcaa gcctggtttc
    15541 agcgcaataa aagtgatggt gtgatcggcg ttgatcaccg cgcctggcgt agcgccagtt
    15601 tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg ggaattagcg
    15661 tggtcgatta actggctaat ggattcgcgg ggcgcttgcc gcaaaccggt accgagcagc
    15721 gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg gatctcgcca
    15781 cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg tttgtcgctc
    15841 tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc gaccacgtag
    15901 ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc gtcaggatac
    15961 gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc atagagtgtg
    16021 agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc gtaccagacg
    16081 gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc agagcccctc
    16141 gatctcaatc agttagcgca aaaaattaaa cagtggggac tggaactggg ctttcagcag
    16201 gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc atggctggac
    16261 aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg cgctcgccct
    16321 catgagttat tgcccggtac gctgcgcgtg atcagcgtgc ggatgaatta ccttcctgct
    16381 aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag ccgttatgcg
    16441 ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg cgagatgatt
    16501 cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc tattctcgag
    16561 cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact tatcctcaat
    16621 cgcgaggccg gttcgttctt ctttttaggc gaattgctgg tcgatattcc gctgcccgtg
    16681 gatcaaccag tcgaggaagg atgcggcaaa tgcgtggcct gtatgacgat ttgcccgacc
    16741 ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta tctcaccatc
    16801 gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg tatttacggt
    16861 tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cacaactcac cacagaagag
    16921 gatttcagcc cgcgtaagcc gctacacgca ccggaactca ttgagttatt cgcctggagc
    16981 gaagagaagt ttttaaaagt cacggaagga tcggcgattc gtcgtattgg tcacctgcgt
    17041 tggctgcgta atatcgccgt agcattaggc aatgcccctt gggatgaaac gattttgaca
    17101 gcgctggaaa gtcgtaaagg tgagcaccca cttctcgatg agcacatagc gtgggcgatt
    17161 gcgcagcaaa tagagagacg aaatgcgtgc atagtcgaag tgcaattgcc gaaaaaacag
    17221 cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat ccacagcttg
    17281 tgaataaaaa taaaaacgca ttgcaattca agacgaataa aatcgtcaag tgatcgactc
    17341 gacaaaacgg aatcttaatt tttaacttat ctatcaatag gttaaaaaaa tactattcac
    17401 cgtgcgaagt ttctttgaaa ttaagattac agcctgaggc tgtggataac tctgtttaca
    17461 agagtttgtc agagacaacg aaaaccagca ataacgcatc gcatcgctgt ggatatttta
    17521 ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
    17581 gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac agcactttca
    17641 aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
    17701 taccaactga gctattcccg cttgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatc gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctga ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taaaaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcagac tggtgtactg caatggtcgg cccttctgcc agaatattca
    18481 ggttggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtctgta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaatgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggctcgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt tggccggatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggct gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccatcgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg taccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattg gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cccgccccac ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gataaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgctcc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacga gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac tggcaatacc ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ccgcacgcct gaaagcgctc gttgatgaac tggaactggc gcagctgtct gccaataacc
    21421 gtcaggaatt agcgcgctta cgctcagagc tggcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga acagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaattg ctggcagaaa acagcgccga tttgccgaaa gacatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cggcactgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgccaggcca ccagccagac gttacaggtt cggcaggcgc
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caacctgcac ggcgaagcgt
    21781 tgcgggcgca ggtggcacgg ctgccggaaa tgccgaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat tcatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacagct acgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactgctgga actgaccaag ctgaaagtct ccaacgggca acttgaggat gcgctgaaag
    22081 aggtgaatga agcgacgcat cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc actggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacgatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gtcggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgctgcc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggcgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgtgt ttcccgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gacggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat cccactgtat ctcaacaaag
    22801 agggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgt cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgcgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gggcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc ggtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttctct gtgggatgtc acttccactg
    23281 tacagggcgt agaaagcctg gagccgatta ccctcggtgc agtgctaatt gccattctgg
    23341 tatttatcat caccacgcag ctggtgcgca acctgccagc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gttacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcaatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggttgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtggtgtt gacgataccg gcccctgccg
    23821 atgccaatag cgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacagggg attcagattt
    23941 tcgagctgcg tatttacgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggcaggc ttccacgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaagttt gtgtgccgga tgaaccaatc cggctcacaa
    24181 acaaaactga tagcgcagaa aaaactgcca cagccagtag ccgaaaatag ccagcgcggt
    24241 ggcgtacata aaccagccgc gtttttttcg ccacaaagcg aaccagagaa aagggaaaaa
    24301 gagcggtaac gtcattatca caccaacatc acgggtatcg tcttcttcga gatacatcaa
    24361 cacatcgcag acgttgttaa tcccgcgtcc atcaatcatc cactctttgt ccagcgcgga
    24421 agtgatggtt atccagagga acgccacaat aaaccagatg cagcacaaga caaacatgcg
    24481 cgcactcata aggtaaccgg tcgcttaatt tcgctatagc gcatacgaaa caaatcgccg
    24541 tatcccacta attccccgcg aaaaaccaga catcggataa gataagcgca ataaatatcc
    24601 gacaggtttc cccaccgttt accatcgcga tctttgaggg tgttaatggt gctacccacc
    24661 accatcgcca ctttacgggt gtggcaggca tcgacaaaag agagaatcgt ctgctctatt
    24721 tccgctacat ccccggcaga aaattgcagc gccctgattt catcttcggt tattgccggg
    24781 tcccggctgg tgtcatccat aacatttcct gtctttacgc cctatcgtca ggcagacgat
    24841 agggcatcgg gttattccac cgcccactgc gggttttttg ccactctgct gcaatagttc
    24901 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
    24961 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
    25021 accaccagta cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
    25081 aatggtctgt cgagatcctg acgtgcttta aagaatggga aagccagcgc gaggaacagg
    25141 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgcggatgcg
    25201 gtaccgccac caaacgaaac cagcaggatg aagacagtaa ccaacccgca ctgcatccac
    25261 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
    25321 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
    25381 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
    25441 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
    25501 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
    25561 ctgacacccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
    25621 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
    25681 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
    25741 gactgataac ctgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
    25801 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
    25861 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
    25921 accagaatca tccatgccac tgccagcaga ccaaccacct gcgtaggctc cagtccagca
    25981 atacgccagt gctgggtcat gtcgctacca tagaggaatg ttgagaacgg tacccaaact
    26041 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
    26101 aaggcaaaac gtggtccgac actattattc atccaggaat agataccgcc ttcttctttg
    26161 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
    26221 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
    26281 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
    26341 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
    26401 ctgcactgca caacattgag caggcagcgt aaaaaccaat acagaattga tacctggatg
    26461 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
    26521 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
    26581 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
    26641 gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa
    26701 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
    26761 gttcaaagcg ttgttgctgc tcacgggcat ccgtcaattc atggaaacca ttcgccagct
    26821 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
    26881 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa
    26941 tatttggctc tacgccaaag gtaaacagca attgtagcag cgtgtcgcgg tcttcttcgg
    27001 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
    27061 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
    27121 ctgccgggca gtccagcatc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
    27181 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
    27241 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg gcaaccagca
    27301 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
    27361 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
    27421 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
    27481 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
    27541 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
    27601 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
    27661 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
    27721 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
    27781 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
    27841 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
    27901 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
    27961 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
    28021 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
    28081 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
    28141 ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc gtcgcccgga
    28201 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
    28261 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
    28321 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    28381 cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg ctaacgcggt
    28441 cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    28501 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    28561 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    28621 cggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    28681 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    28741 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg
    28801 tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc atgaacgtct
    28861 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    28921 tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    28981 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    29041 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    29101 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    29161 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    29221 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    29281 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    29341 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    29401 gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    29461 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct
    29521 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    29581 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    29641 taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    29701 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    29761 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    29821 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    29881 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta
    29941 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    30001 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    30061 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    30121 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    30181 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    30241 agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg
    30301 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    30361 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    30421 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    30481 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    30541 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    30601 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagct
    30661 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    30721 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    30781 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    30841 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    30901 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    30961 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    31021 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    31081 cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc
    31141 tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta tcgccaatgt
    31201 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    31261 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    31321 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    31381 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    31441 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    31501 tttacgggcg tgcttggtgg cgaggctatt gctcgagggg aaatcaagtt aagcgatccc
    31561 acaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    31621 ctcgcaacct acactgctgg cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca
    31681 agcgacttgc tgcgcttcta tcaaaactgg aagcctgcat gggcgccagg aacacaacgt
    31741 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    31801 agttttgagc aggcgatgca aacacgtgtc ttccagccac tcaaactcaa ccatacgtgg
    31861 attaatgtac cgcccgcaga agaaaagaat tacgcctggg gatatcgcga aggcaaggca
    31921 gttcatgttt cgccaggggc gttagatgct gaaacttatg gtgtgaagtc gaccattgaa
    31981 gatatggcct gctgggtacg aagcaatatg aatccccgtg atatcaacga caaaacactt
    32041 cagcaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    32101 ctgggctggg aaatgctgga ctggccggta aatcctgaca gcatcattaa cggcagtggc
    32161 aataaaattg cactggcagc acaccccgta aaagcgatta cgcccccaac tcctgcagta
    32221 cgcgcatcat gggtacataa aacaggggca accggcggat ttggtagcta tgtcgcgttt
    32281 attccagaaa aagagctggg tatagtgatg ctggctaaca aaaactatcc caatccagcg
    32341 agagtcgccg ccgcctggca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    32401 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    32461 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    32521 tgcctgcagt tctcctacgc cgccgcgtgg cgtaacagta gcgaataatt tcgacgccaa
    32581 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    32641 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    32701 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    32761 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggctataa
    32821 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    32881 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    32941 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    33001 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    33061 gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatagccg cgccgactgc
    33121 gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca tcgcccaagc
    33181 aagtagcgcc atactgacaa tcatcgccgt cacggtaata acactcggcg tcaaacgact
    33241 aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac
    33301 taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg tgaaaagagg
    33361 cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg
    33421 aatttcaact attaccttag tgactatcgc agaaaaaaca aacggcacga catacctgtc
    33481 gtaccgttat tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac
    33541 cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg
    33601 aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt
    33661 aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg
    33721 acggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga
    33781 gttgccgaga tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag
    33841 cgtgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg
    33901 atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc
    33961 agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa ccttaaccac
    34021 agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc
    34081 cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg
    34141 accattccac agagttacga tacactctgc ctgatccagc agccatttag cgttgtcgcc
    34201 aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa
    34261 ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga
    34321 atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac
    34381 gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg
    34441 ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat
    34501 aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc
    34561 taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg
    34621 atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg
    34681 ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca
    34741 atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt
    34801 ccggattctc caccgacccg ctggaagaac agcacagcgt agtgcctggt ttgttgcata
    34861 aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt
    34921 gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg
    34981 cacttgagta tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc
    35041 cgctgatggc gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc
    35101 atatcaaacg tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg
    35161 aggcgctggt tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca
    35221 atcatgccaa tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg
    35281 gtgtcaccct gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgt
    35341 tggcaaacct gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc
    35401 tagataaagt acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta
    35461 tgcgtgagtt gctgacattg gtgtcgggat atctggtgcc gaaactggcg cgagaaattg
    35521 gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac
    35581 agggaagtta aacatt
//
LOCUS       DAZFAH010000026        34975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFAH010000026 DAZFAH010000000
VERSION     DAZFAH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.026"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            209..1525
                     /gene="fucP"
                     /locus_tag="ACHJWB_001217"
     CDS             209..1525
                     /gene="fucP"
                     /locus_tag="ACHJWB_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4312884.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYATIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1558..3333
                     /gene="fucI"
                     /locus_tag="ACHJWB_001218"
     CDS             1558..3333
                     /gene="fucI"
                     /locus_tag="ACHJWB_001218"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4312885.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3442..4859
                     /gene="fucK"
                     /locus_tag="ACHJWB_001219"
                     /pseudo
     CDS             3442..4859
                     /gene="fucK"
                     /locus_tag="ACHJWB_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            4861..5283
                     /gene="fucU"
                     /locus_tag="ACHJWB_001220"
     CDS             4861..5283
                     /gene="fucU"
                     /locus_tag="ACHJWB_001220"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4312886.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5341..6072
                     /gene="fucR"
                     /locus_tag="ACHJWB_001221"
     CDS             5341..6072
                     /gene="fucR"
                     /locus_tag="ACHJWB_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4312887.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETMCRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6116..7216)
                     /gene="rlmM"
                     /locus_tag="ACHJWB_001222"
     CDS             complement(6116..7216)
                     /gene="rlmM"
                     /locus_tag="ACHJWB_001222"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4312888.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7209..7604)
                     /locus_tag="ACHJWB_001223"
     CDS             complement(7209..7604)
                     /locus_tag="ACHJWB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4312889.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7623..8540)
                     /gene="gcvA"
                     /locus_tag="ACHJWB_001224"
     CDS             complement(7623..8540)
                     /gene="gcvA"
                     /locus_tag="ACHJWB_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4312890.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8891..9118)
                     /locus_tag="ACHJWB_001225"
     CDS             complement(8891..9118)
                     /locus_tag="ACHJWB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4312891.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9310..10515
                     /gene="csdA"
                     /locus_tag="ACHJWB_001226"
     CDS             9310..10515
                     /gene="csdA"
                     /locus_tag="ACHJWB_001226"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4312892.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSKLLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10515..10958
                     /gene="csdE"
                     /locus_tag="ACHJWB_001227"
     CDS             10515..10958
                     /gene="csdE"
                     /locus_tag="ACHJWB_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4312893.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11009..11815)
                     /gene="tcdA"
                     /locus_tag="ACHJWB_001228"
     CDS             complement(11009..11815)
                     /gene="tcdA"
                     /locus_tag="ACHJWB_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4312894.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12054..13151)
                     /gene="mltA"
                     /locus_tag="ACHJWB_001229"
     CDS             complement(12054..13151)
                     /gene="mltA"
                     /locus_tag="ACHJWB_001229"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4312895.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13360..13436
                     /locus_tag="ACHJWB_001230"
     tRNA            13360..13436
                     /locus_tag="ACHJWB_001230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13394..13396,aa:Met,seq:cat)
     gene            complement(13511..14764)
                     /gene="amiC"
                     /locus_tag="ACHJWB_001231"
     CDS             complement(13511..14764)
                     /gene="amiC"
                     /locus_tag="ACHJWB_001231"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4312896.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKSQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            14996..16327
                     /gene="argA"
                     /locus_tag="ACHJWB_001232"
     CDS             14996..16327
                     /gene="argA"
                     /locus_tag="ACHJWB_001232"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4312897.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16389..18215)
                     /gene="recD"
                     /locus_tag="ACHJWB_001233"
     CDS             complement(16389..18215)
                     /gene="recD"
                     /locus_tag="ACHJWB_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4312898.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18215..21757)
                     /gene="recB"
                     /locus_tag="ACHJWB_001234"
     CDS             complement(18215..21757)
                     /gene="recB"
                     /locus_tag="ACHJWB_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4312899.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRDTVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARKIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWELVLTAWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21750..24638)
                     /gene="ptrA"
                     /locus_tag="ACHJWB_001235"
     CDS             complement(21750..24638)
                     /gene="ptrA"
                     /locus_tag="ACHJWB_001235"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4312900.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIVLSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYSSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24814..28182)
                     /gene="recC"
                     /locus_tag="ACHJWB_001236"
     CDS             complement(24814..28182)
                     /gene="recC"
                     /locus_tag="ACHJWB_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4312901.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKIYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMGAIVEQSQRFLLPLFRFNQS"
     gene            complement(28195..28518)
                     /locus_tag="ACHJWB_001237"
     CDS             complement(28195..28518)
                     /locus_tag="ACHJWB_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4312902.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPTNWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28503..28910)
                     /locus_tag="ACHJWB_001238"
     CDS             complement(28503..28910)
                     /locus_tag="ACHJWB_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4312903.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVTMESQS
                     LRRQAIVQSALEWGKMHAWQVQQAVQCSQYAATGARACLRRLADNKTLLIAGYEDVSL
                     WRMGEVIDGNIVFSPRGWIDFCPLKEGALCQLP"
     gene            complement(28907..29470)
                     /locus_tag="ACHJWB_001239"
     CDS             complement(28907..29470)
                     /locus_tag="ACHJWB_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4312904.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(29461..29931)
                     /locus_tag="ACHJWB_001240"
     CDS             complement(29461..29931)
                     /locus_tag="ACHJWB_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4312905.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30116..30910)
                     /gene="thyA"
                     /locus_tag="ACHJWB_001241"
     CDS             complement(30116..30910)
                     /gene="thyA"
                     /locus_tag="ACHJWB_001241"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4312906.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVSDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30917..31792)
                     /gene="lgt"
                     /locus_tag="ACHJWB_001242"
     CDS             complement(30917..31792)
                     /gene="lgt"
                     /locus_tag="ACHJWB_001242"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4312907.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(31943..34189)
                     /gene="ptsP"
                     /locus_tag="ACHJWB_001243"
     CDS             complement(31943..34189)
                     /gene="ptsP"
                     /locus_tag="ACHJWB_001243"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4312908.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34202..34732)
                     /gene="rppH"
                     /locus_tag="ACHJWB_001244"
     CDS             complement(34202..34732)
                     /gene="rppH"
                     /locus_tag="ACHJWB_001244"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4312909.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         8443 a         9543 c         8712 g         8277 t
ORIGIN      
        1 ttaaagtgat ggtagtcaca taaagtcacc ttccagctaa taagtgtgac cgccgtcata
       61 ttacagagcg ttttttattt gaaaatgaat ccatgagttc atttcagaca ggcaaatatt
      121 cactgatatg aagcccgaac tcgctggttt tgcacttttg aaaacataac cgattacgtg
      181 cttaagcttc tgaacctaag aggatgctat gggaaacaca tcaatacaaa cgcagagtta
      241 ccgtgcggta gataaagatg cagggcaaag cagaagttac attattccat tcgcgctgct
      301 gtgctcactg ttttttcttt gggcggtagc caataacctt aacgacattt tattacctca
      361 attccagcag gcttttacgc tgacaaattt ccaggctggc ctgatccaat cggcctttta
      421 ctttggttat ttcattatcc caatccctgc tgggatattg atgaaaaaac tcagttataa
      481 agcagggatt attaccggat tatttttata tgccttgggc gctgcattat tctggcccgc
      541 tgcagaaata atgaactaca ccttattttt agttggccta tttattattg cagccggatt
      601 aggttgtctg gaaactgccg caaacccttt tgttacggta ttagggccgg aaagcagtgg
      661 tcacttccgc ttaaatcttg cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt
      721 ctttgggcaa agtcttattt tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa
      781 aatgtctcca gagcaattga gtgcgtataa acacagcctg gtattatcgg tacagacacc
      841 ttatatgatc atcgtggcta tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt
      901 cccggcattg cagagtgata atcacagtga cgccaaacaa ggatcgttct ccgcatcgct
      961 ttctcgcctg gcgcgtattc gccactggcg ctgggcagta ttagcgcaat tctgctacgt
     1021 tggcgcacaa acggcctgct ggagctattt gattcgctac gctgtagaag aaattccagg
     1081 tatgactgca ggctttgccg ctaactattt aaccggaacc atggtgtgct tctttattgg
     1141 tcgtttcacc ggtacctggc tcatcagtcg cttcgcacca cacaaagtcc tggccgccta
     1201 cgcattaatc gctatggcac tgtgcctgat ctcagccttc gctggcggtc atgtgggctt
     1261 aatagccctg actttatgca gcgcctttat gtcgattcag tacgcaacaa tcttctcgct
     1321 gggcattaag aatctcggcc aggacaccaa atacggttcg tccttcatcg ttatgaccat
     1381 cattggcggc ggtattgtca ctccggtcat gggttttgtc agtgacgcgg cgggcaacat
     1441 ccccactgct gaactgatcc ccgcactctg cttcgcggtc atctttatct ttgcccgttt
     1501 ccgctctcaa acggcaacta actgaacata ttttccgaat aaagtgagga atctgtaatg
     1561 aaaaaaatca gcttaccgaa aattggtatc cgcccggtta ttgacggtcg tcgcatgggt
     1621 gttcgtgagt cgcttgaaga acaaacaatg aatatggcga aagctacggc cgcactgctg
     1681 accgagaaac tgcgccatgc ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt
     1741 atcgcgggta tggctgaagc cgctgcttgc gaagaaaaat tcagcagtca gaatgtaggc
     1801 ctcaccatta cggtaacgcc ttgctggtgc tatggcagtg aaaccatcga catggatcca
     1861 acccgcccga aagccatttg gggctttaac ggcactgaac gtcccggcgc tgtttacctg
     1921 gcagcggctc tggcagctca cagccagaaa ggcatcccgg cattctccat ttacggtcat
     1981 gacgttcagg atgccgatga cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc
     2041 tttgcccgcg ccggtttggc cgtcgccagc atgaaaggta aaagctatct gtcgctgggc
     2101 ggcgtttcga tgggtatcgc cggttctatt gttgatcaca acttctttga atcctggctg
     2161 ggaatgaaag tccaggcggt ggatatgacc gaactgcgtc gccgtatcga tcagaagatt
     2221 tacgacgaag ccgaactgga aatggcactg acctgggctg ataaaaactt ccgctatggc
     2281 gaagatgaaa ataacaaaca gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc
     2341 gaaagtttac tgatggcgat gtgtatccgc gacatgatgc aaggcaacag caaactggcc
     2401 gatattggtc gcgtggaaga atcacttggc tacaacgcca tcgctgcggg cttccagggg
     2461 caacgtcact ggaccgatca atatcccaat ggtgacaccg ccgaagcgat cctcaacagt
     2521 tcatttgact ggaatggcgt gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt
     2581 aacggcgtgg caatgctaat gggtcaccag ctcaccggca ccgctcaggt atttgccgat
     2641 gtgcgtacct actggtcacc agaagcaatt gagcgtgtaa cggggcataa actcgatgga
     2701 ctggcagaac acggcatcat ccatttgatc aactcaggtt ctgctgcgct ggacggttcc
     2761 tgtaaacaac gcgacagcga aggtaacccg acgatgaagc cgcactggga aatctctcag
     2821 caagaggctg acgcttgcct cgccgctacc gaatggtgcc cggcgatcca cgaatacttc
     2881 cgtggcggcg gttactcttc ccgcttcctt accgaaggcg gcgtcccgtt caccatgact
     2941 cgtgtcaaca tcatcaaagg cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg
     3001 gaattgccga aggatgtgca tgacatcctc aacaaacgca ccaactcaac ctggccaacc
     3061 acctggtttg caccgcgcct caccggtaaa gggccgttta cggatgtgta ctcggtaatg
     3121 gcgaactggg gcgctaacca tggggttctg accatcggcc acgttggcgc tgactttatc
     3181 actctcgcct ccatgctgcg tatcccggta tgtatgcaca acgttgaaga gaccaaagtg
     3241 tatcgtcctt ctgcctgggc tgcgcacggc atggatattg aaggccagga ttaccgcgct
     3301 tgccagaact acggcccgtt gtacaagcgt taatacatta ctccctgatg tgatgcccgg
     3361 tcgctccggc taccgggcct gaacaagcaa gagtggttag ccggataagc aatgttatcc
     3421 ggctatattg caggagcgat tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg
     3481 accaatgtca gggccatcgc ggttaatcgg cagggaaaat tgttgcccgc gcctcaacgc
     3541 ctaatgccag cgatatcgcg atggaaaaca acacctggca ccagtggtct ttagacgcca
     3601 ttttgcaacg ctttgctgat tgctgtcggc aaatcaatag tgaactgact gaatgccaca
     3661 tccgcggtat cgccgtcacc acctttggtg tggatggcgc tctggtagat aagcaaggca
     3721 atctgctcta tccgatcatt agctggaaat gtccgcgaac agcagcggtt atggacaata
     3781 ttgaacggtt aatctccgca cagcggttgc aggctatttc tggcgtcgga gcctttagtt
     3841 tcaatacgtt atataagttg gtgtggttga aagaaaatca tccacaactg ctggaacgcg
     3901 cgcacgcctg gctctttatt tcgtcgctga ttaaccaccg tttaaccggc gaattcacta
     3961 ctgatatcac gatggccgga accagccaga tgctggatat ccagcaacgc gatttcagtc
     4021 cgcaaatttt acaagccacc ggtattccac gccgactctt ccctcgtctg gtggaagcgg
     4081 gtgaacagat tggtacgcta cagaacagcg ccgcagcaat gctcggctta cccgttggca
     4141 taccggtgat ttccgcaggt cacgataccc agttcgccct ttttggcgct ggtgctgaac
     4201 agaatgaacc cgtgctctct tccggtacat gggaaatttt aatggttcgc agcgcccagg
     4261 ttgatacttc gctgttaagt cagtacgccg gttccacctg cgaactggat agccaggcag
     4321 agttgtataa cccaggtatg caatggctgg catccggcgt gctggaatgg gtgagaaaac
     4381 tgttctggac ggctgaaaca ccctggcaaa tgttgattga agaggctcgt ctgatcgcgc
     4441 ctggcgcgga tggcgtgaaa atgcagtgtg atttattgtc gtgtcagaac gctggctggc
     4501 aaggagtgac gcttaatacc acgcgggggc atttctatcg cgcggcgctg gaagggttaa
     4561 ctgcgcaatt acagcgcaat ctacagatgc tggaaaaaat cgggcacttt aaggcctctg
     4621 aattattgtt agtcggtgga ggaagtcgca acacattgtg gaatcagatt aaagccaata
     4681 tgcttgatat tccggtaaaa gttctcgacg atgccgaaac gaccgtcgca ggagctgcgc
     4741 tgttcggttg gtatggcgta ggggaattta acagcccgga agcagcccgt gcacagattc
     4801 attatcagta ccgttatttc tacccgcaaa ctgaacctga atttatagag gaagtgtgaa
     4861 atgctgaaaa caatttcgcc gttaatttct cccgaactat tgaaagtgct ggcagagatg
     4921 ggacatggag atgaaattat tttttccgat gctcactttc ccgcccattc gatgggaccg
     4981 caggtgatcc gcgctgatgg cctgttggtg agcgacttgc tccaggcgat tatcccgtta
     5041 tttgaactgg acagttatgc accgccgctg gtgatgatgg cggcggtaga aggtgacact
     5101 ctcgatcctg aagtagaacg acgttaccgt aatgcgcttt cactacaagc cccgtgtcct
     5161 gacatcatcc gcatcaatcg ttttgcgttt tatgaacggg cgcaaaaagc ctttgcgatc
     5221 gttatcacag gcgaacgagc gaagtacggg aatattcttt taaaaaaagg ggtaacaccg
     5281 taatctcata ccggtacgcc cgcatgacgc gggcggttat cgaatgatgg ggtgaaaaat
     5341 atgaaagcgg cacgccagca agcgatagtc gacctgctgc tgaaccatac cagcctgacc
     5401 acggaagctc tctctgaaca gctaaaggtc agtaaagaaa ccatgtgtcg cgatctcaat
     5461 gaattacaga cgcagggtaa aattctgcgc aatcatggac gcgctaaata tatccaccgt
     5521 caaaatcaag acagtggcga tccctttcac atcaggctga aaagccatta tgcgcataaa
     5581 gcagatatcg cgcgcgaggc gctcgcgtgg attgaagaag ggatggtgat agccttagac
     5641 gccagttcaa cttgctggta tctggcacgc cagttgcctg acatcaacat tcaggtcttc
     5701 accaatagcc atccgatttg ccatgaactc ggtaaacgcg aacgcattca actgatcagt
     5761 tcaggcggca cacttgagcg caaatatggc tgttacgtca atccctcgct gatttcccaa
     5821 cttaaatcgc tggaaatcga tctgtttatt ttttcttgtg aagggatcga tagcagcggc
     5881 gcactgtggg actccaatgc gatcaacgct gattacaaat cgatgctatt aaaacgtgcc
     5941 gcgcaatcgt tgttattgat tgataaaagt aaatttaatc gttcagggga agcccgcatc
     6001 gggcatctgg atgaggtaac gcacattatt tctgatgagc gccaggttgc aacttctttg
     6061 gtaacagcct gacggaaagg gtagcaggcc ggagacgacg ccccggcctt gcctgttatc
     6121 gctcgtcgcg acgaccaccc accgcagccc agatgcggcg gacgtgcacc gtcacttctt
     6181 cgcgatcgtg atacaactgc cgcgcctgaa tctgagcatt tatgccatgt tcatcaagct
     6241 gtgcctgaat atacgccaga ttgtgtgaca cttcttcgta gcgttttttc atcggcagtt
     6301 tgaggttgaa aatggtttca cggcaccagc cattaaccag ccactgcgcc atcaatgccg
     6361 caactttcgc cggtttttca accatatcgc ataccatcca ggagatattg ctgcgcgtcg
     6421 gacggaattt gaaaccgtct tcccgcagcc acgtcacctg tccggtatcc atcagacttt
     6481 gcgccatcgg gccgttgtcg acggaataaa cccacatgtt gcgcttcacc agttggtagg
     6541 tccagccgcc agggcaagcg cctaaatcca ccgcccacat cccgttcgcc aggcgttcat
     6601 cccactcatc cgcaggaata aacacatgaa atgcctcttc cagtttgagc gtggaacgac
     6661 tcggcgcatc tgccggaaat ttcaggcgcg gaatgcccat atagaacggc gaattattgt
     6721 tgctgtatga gtaaccggta tagcagcagc ctggtgcaat aaagaataca tgcacaaccg
     6781 gacgcttcgg cgtttcatag ttcgccagca ccccggcatc gcgcaaggca gcgcgtagcg
     6841 gcacggtgaa tttacggcag aatttcagta actctttgct ttcgttggta tcggcaactt
     6901 caacacgcag ttcaccgccc ttctctacta cgccctgtaa catgccgaca atgggggtaa
     6961 tacgatcttc tggcggcaaa tgctgcagga gttcccccac cacaaaccac tggcgggcaa
     7021 aaattaatga actgaacggc agctcacgga ttaacttatc gccatcatca ggttgataac
     7081 attcataaat gacataaccc gcattctctt tcacgcgggc aaaaccgaaa atttcccgct
     7141 ggccggcttt atcggtaatt tctgcggcgc actctttttc aaagcccgga cggcacagca
     7201 atacaacctt attcatgact tacgcccttg cgctttaaac ggatagcacc aactaacatt
     7261 aacgcccagc ccgcgaggaa gctcacgccg ccaaccggag tgacaaacgc ccacaaacgc
     7321 agatgggaca gcgccaggca ataaaggctg ccgctgaaca acaccgtgcc taacgcgagg
     7381 aaaacgctac tccagtaaaa ccagatactg atgcgacgct gcattgccac cgccagacct
     7441 aagatcgcca gcgtatgaaa cgcctggtat tcgaggccgg tctggatcca gcccatctca
     7501 acggccccca tggttttact taacacatgc gcgccaaaag cgcccagagc cacaaaaatg
     7561 aagccgctaa tggcggcgaa aatcagcata aaacggctgg tcatggtcgt accctacgta
     7621 aattattgtt cataacgaaa gcggaatttt tcttgttcag cagcggcttt cgccaggatc
     7681 cattggcgaa aggcggctat tttacccagt tctgcctggc tgtcatgaca aaccagataa
     7741 aaagcgtttt tactgaccag aacatcatta aacgggcaaa caagacgtcc ggcctcgatt
     7801 tcagattgcg ccatcacgtt atttgccagc gccactccct gcccgtgaat agccgcttgc
     7861 agcaccatgg cgctatgact aaaaattggc ccttgctgaa cgttgatatg atttaacccc
     7921 aactgtcggg tatatgtctg ccagtcacgg cgtgacgcat catgtaataa cgtatgttta
     7981 gccagatctt ccggggtctt caagggtttt tcgcctgtca gcagtagcgg cgaacacacc
     8041 ggcaataaat attcggcgta cagtttttcc acccgtagcc ccggccagtt gccccgacca
     8101 taaaatatcg ccacatcaac atcatccgcc agcttatctt cctgacgatc aaccgcctgg
     8161 attcgaacgt caattcccgg ataagctgaa ttaaagctcg aaagtcgcgg aaccaaccaa
     8221 tgaatggcga aactggggag taaactgacc gtcaacgccc ccttggcgct acgggcctgg
     8281 agtttacgcg tcgcttcggt taattgcgaa aatatctctt tgatatcgag gaaatagctt
     8341 tgcccttcct cggtcagcag gagtgaacga ttacggcggc ggaacagttt tagccccaaa
     8401 aaatcctcaa gagacttgat ttgatgactt actgcggcct gggtcacaaa aagctcttct
     8461 gctgcgcgag tgaaacttaa atggcgtgct gcggcatcaa aaactcgtaa ggcatttagc
     8521 ggtggtaatc gtttagacat ggcttttaaa ctttgatgtt aaatgaattt aacaattaga
     8581 tcacactgtg taacctatta gtttttttaa tctgagccat tataaattgt ccgttgagct
     8641 tctaccagca aatacctata gtggcggcac ttcctgagcc ggaacgaaaa gttttatcgg
     8701 aatgcgtgtt ctggtgaact tttggcttac ggttgtgatg ttgtgttgtt gtgtttgcaa
     8761 ttggtctgcg attcagacca tggtagcaaa gctacctttt ttcacttcct gtacatttac
     8821 cctgtctgtc catagtgatt aatgtagcac cgcctaattg cggtgctttt ttttaccttg
     8881 cgatcgcgaa ttactgatcc agttcgacca tctctttcac gtcagtacgg ttgatctgct
     8941 gtttgttgcc attagcgtct ttatacgaaa tcataccggt atcgttatca gtctgtggtt
     9001 tgccgtcaga gacgatggta cgtccgtcat tggtgtgcat cacatagttc gaaccggaac
     9061 aggcgctcag ggcaaaagtc agcatacagg cagaaataat tgcggcagtc tttttcatca
     9121 tcttctcctt aaagcgatta ataaatctta atagtattga ttaacaggct aaaattaacg
     9181 cctaacacta ttcagcatat gttacttacg cggcttcgcc aggatatcca gataattctg
     9241 atggttagca ctctccttgt atcaaagtga attttgcgtc acgatcggtg catcaagccg
     9301 aggagtacca tgaacgtttt taatcccgcg cagtttcgcg cccagtttcc cgcactacag
     9361 gatgcgggcg tctatctcga tagcgccgcg accgcgctta aacctgaagc cgtggttgaa
     9421 gccactcgac agttttacag cctgagcgcc ggaaacgtcc atcgcagcca gtttgccgaa
     9481 gcccaacgcc tgaccgcgcg ttatgaagct gcacgagaga aagtggcgca attactgaat
     9541 gcaccggatg ataaaactat cgtctggacg cgcggcacca ctgaatccat caacatggtg
     9601 gcacaatgct atgcgcgtcc gcgtctgcaa ccgggcgatg agattattgt cagcgtggca
     9661 gaacaccacg ctaacctcgt cccctggctg atggtcgccc aacaaactgg agccaaagtg
     9721 gtgaaattgc cgcttaatgc gcagcgactg ccggatgtcg atttgttgcc agaactgatt
     9781 actccccgta gtcggattct ggcgttgggt cagatgtcga acgttactgg cggttgcccg
     9841 gatctggcgc gagcgattac ctttgctcat tcagccggga tggtggtgat ggttgatggt
     9901 gctcaggggg cggtgcattt ccccgcagat gttcagcaac tggatatcga tttctatgct
     9961 ttctctggtc acaaactgta tggcccgacg ggtatcggcg tgctgtatgg caaatcaaaa
    10021 ctgctggaag cgatgtcgcc ctggctgggt ggcggcaaaa tgattcacga agtgagtttt
    10081 gacggcttca cgactcaatc tgcgccgtgg aaactggaag caggaacgcc aaatgtggct
    10141 ggtgtcatag gattaagcgc ggcgctggaa tggctggcag attacgatat caaccaggcc
    10201 gaaagctgga gccgtagttt agcaacgctg gcggaagatg cgctggcgaa acgtccaggc
    10261 tttcgttcat tccgctgcca ggattccagc ctgctggcct ttgattttgc tggcgttcat
    10321 cacagcgata tggtgacgct gctggcggag tacggtattg ccttgcgggc cgggcaacat
    10381 tgcgctcagc cgctactggc agaattaggc gtaaccggca cactgcgcgc ctcttttgcg
    10441 ccatataata caaagagtga tgtggatgcg ctggtgaatg ccgttgaccg cgcgctggaa
    10501 ttattggtgg attaatgacg aacccgcaat tcgccggaca tccgttcggc acaaccgtaa
    10561 ccgcagaaac gttacgcaat accttcgcac cgttgacgca atgggaagat aaatatcgcc
    10621 agttgatcat gctggggaaa cagcttccgg ctttgccaga cgagttaaaa gcgcaggcta
    10681 aagagattgc cggatgcgaa aaccgcgtct ggctgggata taccgtggct gaaaacggta
    10741 aaatgcactt ctttggcgac agcgaaggac gtatagtgcg cggcctgctg gcggtgctgt
    10801 tgactgccgt tgaggggaaa accgccgccg aattgcaggc acagtcgccg ctggcattgt
    10861 ttgatgagct gggattacgt gcgcagctta gcgcctcacg cagccagggg ttaaatgcgt
    10921 taagcgaggc gattatcgct gcgacgaagc aggtttaagc acagaaccgg gtcggataag
    10981 acgttcgcgt cgcatccgac ctgattgttt aaccctgacg cgccgctttc gccatcatct
    11041 tcttcagcgc atgagaaacc gcaacaaaac caaaggtggc ggtcaccatc gttgccgcgc
    11101 caaatccaga tgcacaatcc atccgctttg gcccttccgc cgtggctttc attgcacaca
    11161 ccgtaccgtc tgactgcggg tacaccagcg cttcagtaga aaacacgcaa tccacgccga
    11221 gcttaccttt actgtttttc actacgccaa aatcgctttt caggcgctcg cgcaacttcg
    11281 ccgccagcgg gtcctgaatc gttttcgcca gatcggtaac ctgaatctgc gtcgggtcaa
    11341 tttgcccacc cgcgccaccg gtcgtgacca gcgggatttt attgcgccga caataagcaa
    11401 tcagcgccgc tttgggccgt acactatcaa tggcatcaat cacgtacgaa taaccggcac
    11461 tcatatattg cgctacgtta tccggcgtca cgaaatcatc caccaccgtt acacggcact
    11521 ccgggttaat ctggcgaata cgttccgcca tgacttccgc ttttgccagc ccgacgttat
    11581 cacgcagagc gtgaatttgg cgattggtat tggtgacgca cacatcatcc atatcgataa
    11641 gcgtgattgc gccaatcccc gtgcgcgcca gcgcttccgc cgcccaggaa ccgacaccgc
    11701 caataccgac cacacaaata tgcgcgtcag caaacagttg caacgctttt tcaccataca
    11761 gacgcgctgt gccaccaaaa cgctgacgcc atgcatcact aattaccaca gacatagcac
    11821 ctcagaatta aaaagggcga ggttatccct caccctaaac gcataaatgc ctgatgcgct
    11881 acgcttatca ggcctacata actcctgcaa tattttggat ttgcatgatt tgtaggccgg
    11941 ataaggcgtt cacgccgcat ccggcatgaa caacgcgcac ttagtcaata atcaggatgt
    12001 gaggttaccc ctcaccctgt catatccgta aaaacggcat acagaatatc acatcagccg
    12061 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggtta
    12121 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
    12181 cctttgattg cgccaccgac atccagcgcc accatcagac gcaattcgta ctgaccgtta
    12241 aatttgccgt tattatccag cagcggcact tctgccagca aggttgtacc tggcggaata
    12301 attgaacgat cggaggcaac agaagcacgg ccaaccagcg gcaccgcact tgcccctttc
    12361 accggtgcaa aagattgcgg tttaaagaaa acgaaagacg ggttctgttc cagcagctcg
    12421 cgaacctcgg cttcactgtg tgtttcgccc cagtgacgaa tcgcctgcat cgacatatct
    12481 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
    12541 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
    12601 ctaccctgca catccataat gaagttatcc atcagggagt tactgtaagc gagaatatat
    12661 ttatcactca atgcccccgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
    12721 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
    12781 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
    12841 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
    12901 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
    12961 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctgattc
    13021 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
    13081 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
    13141 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
    13201 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgtacaa aaaacaggct
    13261 ttagtgattt gtttttgttc aaaatcatgc cagatccgtg atcggggtaa aaaaaaggtt
    13321 gcatgaaaac gcgagcggag tatagtgcgc ttccacggac gcggggtgga gcagcctggt
    13381 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta
    13441 ttgaacaccc taacgggtgt ttttttgttt ctggtctccc ataaaaaaag cgccattcag
    13501 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
    13561 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
    13621 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatca ggcgctttca
    13681 gcacggcaaa cccggcctgt tcaacctgat ttttatgcag cttgttgatt ttaccgagct
    13741 tattcagcac cgctttacca aacttcaggc tgtcggcaat ggtcagcgac tgcaccatat
    13801 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtcgg
    13861 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga
    13921 gcgcaaacac agaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
    13981 caaacaggtc agcacgctgt ttctgggatt ttgctacgcg cacttgcaac ggaatgaaga
    14041 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
    14101 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
    14161 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
    14221 ctttacctgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
    14281 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
    14341 ggtccataac cagacgctct ttaaacccgg cgaccggcgc aagtgcaaac agctgcggtt
    14401 ttacgttttg ctttaattca aaaaccatgc gtacggtttg cgggtcaaat tgcccgacgc
    14461 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
    14521 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
    14581 gcttatattt cagctgacga tttgattcca ccgtcacgcg ggtgtagctg gacgcaggcc
    14641 agacgcgcac cgccacgacc tggctgaccg cagccagact gacctgactt acgctcaata
    14701 gccacatggc acccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
    14761 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
    14821 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
    14881 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
    14941 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaagaggt gtgccgtggt
    15001 aaaggaacgt aaaaccgaac tggtcgaggg attccgccat tcggttccct atatcaatac
    15061 ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
    15121 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
    15181 ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaacacg aaccgctgta
    15241 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
    15301 aacattgcaa ctggatatta ccgctcgcct gtcgatgagt ctcaataaca cgccgctgca
    15361 gggcgcgcat atcaacgtcg tcagtggcaa ttttattatt gcccagccgc tgggcgtcga
    15421 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatcca
    15481 tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
    15541 cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc
    15601 tgaaaagatg attggttttt gctcttccca gggcgtcact aatgacgacg gtgatattgt
    15661 ctccgaactt ttccctaacg aagcgcaagc gcgggtagaa gcccaggaag agaaaggcga
    15721 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
    15781 gcgtcgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc
    15841 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
    15901 aatcaacgat atcggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
    15961 ggtacgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg
    16021 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
    16081 aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct
    16141 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac
    16201 gcgcagtatt cactggttcc aggaacgtgg atttacccct gtggatattg atttactgcc
    16261 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
    16321 agggtaagcg atgaaaatcg ccggatgcgg caggcgtaac actcgtgcgt cgcatccggc
    16381 aattacgttt attcccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga
    16441 gtggcgattg ccgcacttaa tatacgctca tcggcataca gcgacagacg gcgacgcgcg
    16501 cgggtcaccg cggtgtatac cagctctcgc gttactaccg gcgtgcgttg gctcggtaaa
    16561 atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta
    16621 gtttcgtgct ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca
    16681 aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac
    16741 ccaagcgcgc tgtcattacg ggcaatcatc accggtcgac cttcgtacca acgagagtgc
    16801 ggatgacgat gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca
    16861 gccacgccaa acggcccttc ccgcagggcg cacaaaagct ggtactcatt gaacgcctga
    16921 atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc ataacccgca
    16981 agagcttcct caagcatcgc aatataatct tcgccgctct gtaaaagccg tttttcgata
    17041 tcagtaaaat cctgctgaaa aacggttttc actgccgttt tatcaccacg gttaatcgcc
    17101 gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag
    17161 agactgtcgc gcaaagatgc cgcttctgtg ccagttcctg ccggaacgtg agttcccgtc
    17221 aggcggctta gctgcccggc acgctcggcg gtaaagccgg cattggcata agcgcagata
    17281 tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc
    17341 actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt
    17401 gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa
    17461 cgctggctac ccggctgcgc acccagcaat cggtgcaaag tactggcatc ttccggaatg
    17521 cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt
    17581 aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg
    17641 gccatttgaa ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg
    17701 ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg ccgctgcaac tttttgccag
    17761 ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca
    17821 acctcaatgg catgattcac ttcgttgaaa aagcgtgcca ctgtgcgttc gttacaccac
    17881 atgcgattca aataaagacg atcgccacag aggatcatcg gcgtgggttc atctccccgg
    17941 ctgaccgctt gtgaagccag caagcattct tcccaatttt gtagctcacc gatttcactg
    18001 acacaggtcg ccaacagcgg atgcgacgcc tcattatttt ccagtcgtga aagcggcaaa
    18061 cagacgtgtc cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga
    18121 tgttcatctc ccgccacagt cagggcaaac tgcacgtcca gcgggcgtag ctgtttgtgc
    18181 tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccaag gtcataccgg
    18241 caaacatctc atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatcccct
    18301 gttgtggatg ttctttatca acgccacgca ggaacagata aataacgccg ccaaagtggc
    18361 gctcatagtc gtaatcagca atgcgatgac gcagataacg atgcagcgcc agggtataaa
    18421 gctgatattg cagatcatag cggtgtgcct gcatcgccgc tgccatagcc tgttgggtgt
    18481 aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt
    18541 cgtggcggaa caccaggtcg ataaagcctt ttaacatgcc acgtacctgc atgaactcca
    18601 gcggcgggca gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg
    18661 cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc tacctgttta tcgcggtcgg
    18721 aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc
    18781 acgcggtcaa caccagttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta
    18841 cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg
    18901 tccccggtga cgcaccgcgc gggaactgat gaggtgttaa cgtcggttct tcaacgacgc
    18961 tggcaacgcc tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac
    19021 cacgctgctg caaaccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta
    19081 gcgtcctcgc attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac
    19141 cagtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa
    19201 gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga
    19261 cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc
    19321 aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt
    19381 cgacctccgc aagctcgacg ctttctggcg cagcgttaag atccagaaca gcctcaaacg
    19441 agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtgata aacggcagcc
    19501 agaccagcgg atattccagc ccttttgatt tatggatcgt gacaatctgt accagatgtt
    19561 tatcactttc gaggcgcatt tgttggctag aggcattact gtctggctcg aggatatgtt
    19621 gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgtagca
    19681 gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt
    19741 tttctgcaat cttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc
    19801 gccagatttg ccgataacca tcgaactctt cgaccaccgc gtcccacgca tgttcgtcat
    19861 tattcagcgt ttcgatatcc agcgcgttca gccccatcat tgacgttgcc agcgcactac
    19921 gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa caaccaaagc atttcctgcg
    19981 cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg atttccagca
    20041 acgttaaggc atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acactgatgt
    20101 ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc
    20161 cggcttgtag ccagtcgcgg atttgcgcag cacatacctg tgccatggta ctttgataat
    20221 cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac
    20281 ctttaaatac aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta
    20341 tttcgcgaaa catgaacgca tcatcagtct ggctgaaaag cttattcacg ctgttcacca
    20401 ttcctggtgc ggaacgccag ttggtgtcta aagtgtagtg ggcgtgaact tcgctacgcg
    20461 ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc
    20521 caattagcaa caatgcggtt tccggctgat ggtgccagat acggcgaaaa attcggtact
    20581 gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg
    20641 ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt
    20701 catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtatcg cggatctcag
    20761 ccaatgcgcg ggtgatcacc agatcgcgga tcgacaatgg ttctgcaagc agttgatcga
    20821 tcgcctcaaa cagtggatgt cgcggggttt cccccccggc cttcgtgcga tcttctaaga
    20881 aacgctggga gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg
    20941 cccaggcgct gatcttttcg atccatttag cctgattgct acggttaaac ttgcgtcgat
    21001 caataccaga agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt
    21061 ttaccgtatc aatacgcgcc acaatttgcg cgtggcggga agccagcgtt tcatcatcgg
    21121 gcggcggtgc tttgataacc ggcgcttcgc cttgcagata acgattaata tcgcgcagca
    21181 acgcctgcgg ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt
    21241 agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa
    21301 tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc
    21361 aaaagccgtg aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc
    21421 actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gcgttcgtac agtggattgt
    21481 cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccgc
    21541 gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acggtcagcg
    21601 ggcgaggaaa ggcggcggaa ccgcctagtc caagtaacag gcgcaaatag agcgccgcaa
    21661 tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca
    21721 agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt
    21781 gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg
    21841 gctttcccgt tttggctgcc ggaaatctgc gacagaatag ccatgccttg cggctcgacc
    21901 accgcctgat ggaagaaatc agcaagtttt tgcggcgtca gcagttttat ctgggccacg
    21961 attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat
    22021 gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt
    22081 tgcgcaaact cttctggctt catcgcccgc aattttgcct ctgcggttgg gaaaaacgcc
    22141 ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg
    22201 ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa ctgttcttcg
    22261 gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct
    22321 gagctgctgt attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg
    22381 ctgttaccgg ctttttcaaa gatgacggat tgttttttat cgaccacgac atctttgtta
    22441 cgacaccatt ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt
    22501 gcctgggcct cagtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag
    22561 gcgtcgcgat aggccagcac ctctttcaac gtaatggagg gcaaaatttt gcgccgttca
    22621 tctcgcgaga agtatggtac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac
    22681 gctttaccct tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc
    22741 tgatcttccg tagcggtata gctaaagtag ccctcgagca atgcctggaa cagctgcggc
    22801 aggcgctggg tgtaaccatt ggcattaacc ataaggccgt tgttagcatt ggtggaaaaa
    22861 cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa
    22921 tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa
    22981 atcaggctga catcggcttt gggctcgctg gcaaaataac ggcttggcgc atacaccacg
    23041 cgcagattcg actcatcaac aatcagctct ggatggtcgt atttcttctc tgacttaatc
    23101 agcgagaaat cgtcaggaat ataagggtta agctctggca aagagagcac aatgtcggcg
    23161 gcttttttct gccagtcggc gaaagtttgt gcgctgattt tatcgacctg atacggcgca
    23221 tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc tgatatacca aatacgcgca
    23281 ttctgcggcg tcatcatcgc aagacgttct tttactgctt tagcatcgta ccgatcagca
    23341 atattgactg catccaacgt atgctcaaca ggaacgcgaa tcatggtatc tgccagccat
    23401 tcgacgtaat ccatatcacg ggtgattgac ggataacgga agtcgatatc cagcacattc
    23461 gccagttcat cgaagtattg tttatcgatc cctttttcac gtaacagatt gaggtagcta
    23521 aaaatggccg ccacaacctg atcgcgattc gccagacctt tatcggttaa agacgcagag
    23581 atcgctaata caccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca
    23641 actaatccct gcttttgcag ccagtcagaa agtgtacctg ggctgcgatt accaatcaga
    23701 taggtaatca attcatccgt tttactacgg aacttcgctg agttgttatc gatgcgaaac
    23761 tcaacgcgca gaactttacg cggcagtgcc gggacgtaat gaatgataat gcccttttgc
    23821 gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga
    23881 ccaaaagtgt ccgccgccat ttttgccaac tccggcagcg gtttattgct gtaaataacc
    23941 gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagtgc ctgctgcacc
    24001 ggattaccag gtttgtcgct taaagtttcg aggttaccgc ctgaaaactt tgaaccgggg
    24061 tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc
    24121 gccatggtta attcagcgtt cactgcatta cgctcacgtt cggcgtattt cttgtcgagc
    24181 aagggttctg caatagcatc ggccaggcgg tctaccgcac cgggcaaggc gtcgttctca
    24241 acttccagat agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc
    24301 attttgagat attcggccag actgtcagcc tgtgggtact ttttcgaccc catcagactc
    24361 atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg
    24421 ggcaccacca gcgccgatag cgatttaact gcctgcggat cagaaaccag caagaccacc
    24481 ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg
    24541 gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaact
    24601 aacaacaata atgctttgaa ccaggtgctg cggggcattc acggacctca taatcaactt
    24661 tattttctgg ccagattcaa caggttaacg caaatcatct gccagaattt aatcttgtgc
    24721 tgcacgagtc agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt
    24781 aagcggatag attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg
    24841 taacaggaaa cgttgcgact gttcaacgat ggcccccatt gtctctggtg ttaattgccg
    24901 ccagagcctt tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta
    24961 agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc
    25021 gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa
    25081 tggcgcggac atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg
    25141 ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc
    25201 accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc
    25261 cacacttaat aaagagggac gccagcgcaa caagccatcc ggctgcacct gcggcaacca
    25321 gccagttatc tgcacaccgt tgcaagcgag atcaatttcc atactctgcc ccggctggcg
    25381 acaggcaatg actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat
    25441 ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc
    25501 ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag
    25561 tccttccaga ataaatggct cggtgtcggg gatttcgctg tcttcagtac ggaagttcac
    25621 ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc
    25681 cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt
    25741 accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg
    25801 gtagttttgc ggatcaaatg gcatccgggt atggaggcaa gtaagatgcg cttttaccct
    25861 tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat
    25921 gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga
    25981 acgcccgata tagctgatat agattttttg ctgcgcggaa attaacgctt ccaggaacag
    26041 atagcggtca tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc
    26101 aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac
    26161 cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg gcccggcgag
    26221 aaaacgctgg ctgatacgtt cttgatccag gcgctgtgcc agttcatcac gcaatagtga
    26281 cagcggcacc gcatcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg
    26341 ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag
    26401 catatcgcga caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg
    26461 ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc
    26521 gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta
    26581 gcccaacaac atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc
    26641 cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggactcgt tgacccactg
    26701 gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc
    26761 cagcagcgcc agcacatcct ctgacacaaa gcgactgtca ggcagtgata acaggctgat
    26821 aaacgcttcc agtaccggat gcgactgccg cgcccgacgg tcggaaatgg cgtaaggcag
    26881 gtaacgatcc gcaggtgcgc taccaaacac agcctgaata aacggactgt agctgtcgat
    26941 atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc gggtcttcct ccagcatcgc
    27001 cagcaggcga tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt
    27061 gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat
    27121 gttcacacca gcaacggcgc ggttttccag ttccagaatg tcagactgaa tgttatgcag
    27181 caggttatct ggcgtgacat cgacaaaggc gtccagctcc tgactgctct ccagatcgga
    27241 aaggagatag atatagtcgc gccccaactt gccccaagag gccagtagcg ggttgccgac
    27301 atcctgttca ccgtcgctgt taaagagctg tccggcattt tcgctatcgc gaaatagcgg
    27361 caattcgcga tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg ccagataagc
    27421 aggatcttta atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc
    27481 aatatgttta cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc
    27541 gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc
    27601 gataaagcgc tgatagagat tggcgcggtg ccagcgcggt tgtccgagtt catgggtata
    27661 ttccaccagc gccttccaca acggcgcttg ccaggcctgt gcttctccca gcccttcaac
    27721 caaatgtcct gtttcccatt gtgccagcca ctccgggcga tagaccagat actggtcaaa
    27781 caggtccgcc gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag
    27841 ataatgccgc agcagggtaa agtcttcgcg atccagcaat tgcggcagca gagtcatcag
    27901 tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatctccg gtaacacccg
    27961 gacgaacata tcccagataa agctcgcagg cagcggaaaa tcaatgtttg ccgcaatacc
    28021 aaacttttgc gacagggtca tttgcagcca ctgtgccata ccggtacttt gcaccagaat
    28081 catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc
    28141 ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg
    28201 acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt
    28261 aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttagt
    28321 aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta
    28381 ctggtttctg ctggcaagac tgttcattaa tgttcgctgg aaacccgata acgcagtgac
    28441 aatcatcacc atcaacacca tcgccaacat tacctccggc aggctaaagc cttgttgatt
    28501 cttcagggaa gctgacataa cgccccctct ttcagcggac aaaaatcgat ccagccgcgt
    28561 ggcgaaaata cgatattccc atcgatgact tcccccattc gccataatga aacgtcttca
    28621 taaccggcta ttagcaaggt tttattatcc gcaagtcttc gcaggcacgc tcgagcaccc
    28681 gttgcggcat attgcgaaca ctgaacagct tgctgtacct gccaggcatg cattttcccc
    28741 cactcaagcg ccgactgcac aatggcctga cggcgtaatg actggctttc catagtcacg
    28801 cgagaggcaa aactgcgatc ctgctggctc attccttgta acagcaagct gcccaaaacc
    28861 agcagcatca ggaccagtgc cagcgatgaa acaccctttt cgcggttcac aggttgaatc
    28921 ctgtcacgct atagctggca ttcaccacgg tttgcggttc agacttactg gcagcacgca
    28981 tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt
    29041 cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg
    29101 caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcggactctt
    29161 ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt
    29221 caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc
    29281 tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct
    29341 tacgggtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgct gcccccagca
    29401 acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt
    29461 ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac
    29521 caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt
    29581 gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg
    29641 tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac
    29701 aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt
    29761 ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccaaagccg
    29821 ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca tgaccagaat
    29881 cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt
    29941 atggagcgag gagaaaaaaa gacgagagcc agtttctatt tcttcggcgc atcttccgga
    30001 ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaacg
    30061 gagggtaaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    30121 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    30181 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    30241 gttcgcggct taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtgtcgc
    30301 cccccgtcca gacaaaatcg ctcacttcca gatcacactg ctgcgccatc atatgcacca
    30361 gtaacgcgta gctggcaatg ttaaacggca ggccgaggaa aacgtcacag gagcgctgat
    30421 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaacgca tggcacggtg
    30481 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat
    30541 ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatccg
    30601 gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgccg ttttcatcgg
    30661 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct
    30721 gcagaaacca cagcagttca tggatgatgg aacgcaggtg gcaacgttta gttgtcacca
    30781 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    30841 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    30901 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    30961 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgtcccatg ctgatgtact
    31021 gcacccaggc accagtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    31081 acgcgccgta accaatcagg aacaaacctg agacagctcc cattgggcgc ggtttacgaa
    31141 tatacaggtt gaggataata aacagcacta caccttccag caacagctcg taaagctgtg
    31201 atgggtggcg cggcagcaca ccgtaagtat cgaaaatgga ttgccactgc gggttggttt
    31261 gcagcagcaa aatatcttct gtacgggagc cagggaacag catggcaaac gggaagttcg
    31321 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    31381 caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc
    31441 gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    31501 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    31561 gaacataacc aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    31621 caacttcatt tttggtccag ccactgcccg gacgattcgc ccgtcgtgtt gccagccaca
    31681 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    31741 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    31801 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    31861 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    31921 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    31981 gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac
    32041 gctgcgcaag attttctgcg tcggcataat caatgcgccg cagcaggtat tttacccgcg
    32101 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    32161 acatgggatc gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt
    32221 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg
    32281 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    32341 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    32401 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    32461 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctt gtgaccatcg
    32521 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    32581 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    32641 tctcttcgct aatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    32701 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    32761 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    32821 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    32881 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    32941 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    33001 gatcgaccag caattcaccg cgataaccat cgacgatcag cgtccgacga tgcagcaccg
    33061 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    33121 catgggagtt ggctgcgcca tcgcgcacga caacaccgac taagcgatcc tggggcaact
    33181 cagcaagcgt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    33241 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    33301 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    33361 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    33421 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    33481 ctttttgtgc accggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    33541 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    33601 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    33661 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg
    33721 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    33781 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    33841 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    33901 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    33961 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    34021 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    34081 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    34141 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    34201 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    34261 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    34321 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    34381 tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt
    34441 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    34501 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    34561 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    34621 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    34681 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    34741 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    34801 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    34861 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    34921 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atata
//
LOCUS       DAZFAH010000027        34179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFAH010000027 DAZFAH010000000
VERSION     DAZFAH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.027"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            313..1881
                     /gene="cydA"
                     /locus_tag="ACHJWB_001245"
     CDS             313..1881
                     /gene="cydA"
                     /locus_tag="ACHJWB_001245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4312910.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1897..3036
                     /gene="cydB"
                     /locus_tag="ACHJWB_001246"
     CDS             1897..3036
                     /gene="cydB"
                     /locus_tag="ACHJWB_001246"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4312911.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3051..3164
                     /gene="cydX"
                     /locus_tag="ACHJWB_001247"
     CDS             3051..3164
                     /gene="cydX"
                     /locus_tag="ACHJWB_001247"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4312912.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3164..3457
                     /gene="ybgE"
                     /locus_tag="ACHJWB_001248"
     CDS             3164..3457
                     /gene="ybgE"
                     /locus_tag="ACHJWB_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4312913.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3607..4011
                     /gene="ybgC"
                     /locus_tag="ACHJWB_001249"
     CDS             3607..4011
                     /gene="ybgC"
                     /locus_tag="ACHJWB_001249"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4312914.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERVRTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4008..4700
                     /gene="tolQ"
                     /locus_tag="ACHJWB_001250"
     CDS             4008..4700
                     /gene="tolQ"
                     /locus_tag="ACHJWB_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4312915.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4704..5132
                     /gene="tolR"
                     /locus_tag="ACHJWB_001251"
     CDS             4704..5132
                     /gene="tolR"
                     /locus_tag="ACHJWB_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4312916.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5197..6447
                     /gene="tolA"
                     /locus_tag="ACHJWB_001252"
     CDS             5197..6447
                     /gene="tolA"
                     /locus_tag="ACHJWB_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4312917.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAA
                     AEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINN
                     YAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKL
                     AKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6580..7872
                     /gene="tolB"
                     /locus_tag="ACHJWB_001253"
     CDS             6580..7872
                     /gene="tolB"
                     /locus_tag="ACHJWB_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4312918.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7907..8428
                     /gene="pal"
                     /locus_tag="ACHJWB_001254"
     CDS             7907..8428
                     /gene="pal"
                     /locus_tag="ACHJWB_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4312919.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8438..9229
                     /gene="cpoB"
                     /locus_tag="ACHJWB_001255"
     CDS             8438..9229
                     /gene="cpoB"
                     /locus_tag="ACHJWB_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4312920.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9394..9469
                     /locus_tag="ACHJWB_001256"
     tRNA            9394..9469
                     /locus_tag="ACHJWB_001256"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9427..9429,aa:Lys,seq:ttt)
     gene            9619..9694
                     /locus_tag="ACHJWB_001257"
     tRNA            9619..9694
                     /locus_tag="ACHJWB_001257"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9652..9654,aa:Val,seq:tac)
     gene            9698..9773
                     /locus_tag="ACHJWB_001258"
     tRNA            9698..9773
                     /locus_tag="ACHJWB_001258"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9731..9733,aa:Lys,seq:ttt)
     gene            9920..9995
                     /locus_tag="ACHJWB_001259"
     tRNA            9920..9995
                     /locus_tag="ACHJWB_001259"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9953..9955,aa:Lys,seq:ttt)
     gene            10029..10104
                     /locus_tag="ACHJWB_001260"
     tRNA            10029..10104
                     /locus_tag="ACHJWB_001260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10062..10064,aa:Lys,seq:ttt)
     gene            10135..10260
                     /locus_tag="ACHJWB_001261"
     ncRNA           10135..10260
                     /ncRNA_class="other"
                     /locus_tag="ACHJWB_001261"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10509..11552
                     /gene="nadA"
                     /locus_tag="ACHJWB_001262"
     CDS             10509..11552
                     /gene="nadA"
                     /locus_tag="ACHJWB_001262"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4312921.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11590..12309
                     /gene="pnuC"
                     /locus_tag="ACHJWB_001263"
     CDS             11590..12309
                     /gene="pnuC"
                     /locus_tag="ACHJWB_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4312922.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12306..13247)
                     /gene="zitB"
                     /locus_tag="ACHJWB_001264"
     CDS             complement(12306..13247)
                     /gene="zitB"
                     /locus_tag="ACHJWB_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4312923.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13361..13741)
                     /gene="ybgS"
                     /locus_tag="ACHJWB_001265"
     CDS             complement(13361..13741)
                     /gene="ybgS"
                     /locus_tag="ACHJWB_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4312924.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDSTTQ"
     gene            14057..15109
                     /gene="aroG"
                     /locus_tag="ACHJWB_001266"
     CDS             14057..15109
                     /gene="aroG"
                     /locus_tag="ACHJWB_001266"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4312925.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15276..16028)
                     /gene="gpmA"
                     /locus_tag="ACHJWB_001267"
     CDS             complement(15276..16028)
                     /gene="gpmA"
                     /locus_tag="ACHJWB_001267"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4312926.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16231..17271)
                     /gene="galM"
                     /locus_tag="ACHJWB_001268"
     CDS             complement(16231..17271)
                     /gene="galM"
                     /locus_tag="ACHJWB_001268"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4312927.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17265..18413)
                     /gene="galK"
                     /locus_tag="ACHJWB_001269"
     CDS             complement(17265..18413)
                     /gene="galK"
                     /locus_tag="ACHJWB_001269"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4312928.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMEESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18417..19463)
                     /gene="galT"
                     /locus_tag="ACHJWB_001270"
     CDS             complement(18417..19463)
                     /gene="galT"
                     /locus_tag="ACHJWB_001270"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4312929.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19473..20489)
                     /gene="galE"
                     /locus_tag="ACHJWB_001271"
     CDS             complement(19473..20489)
                     /gene="galE"
                     /locus_tag="ACHJWB_001271"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4312930.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20750..22222)
                     /gene="modF"
                     /locus_tag="ACHJWB_001272"
     CDS             complement(20750..22222)
                     /gene="modF"
                     /locus_tag="ACHJWB_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4312931.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLTDCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22290..23078)
                     /gene="modE"
                     /locus_tag="ACHJWB_001273"
     CDS             complement(22290..23078)
                     /gene="modE"
                     /locus_tag="ACHJWB_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4312932.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23207..23356
                     /gene="acrZ"
                     /locus_tag="ACHJWB_001274"
     CDS             23207..23356
                     /gene="acrZ"
                     /locus_tag="ACHJWB_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4312933.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23522..24295
                     /gene="modA"
                     /locus_tag="ACHJWB_001275"
     CDS             23522..24295
                     /gene="modA"
                     /locus_tag="ACHJWB_001275"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4312934.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24295..24984
                     /gene="modB"
                     /locus_tag="ACHJWB_001276"
     CDS             24295..24984
                     /gene="modB"
                     /locus_tag="ACHJWB_001276"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4312935.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLELEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24987..26045
                     /gene="modC"
                     /locus_tag="ACHJWB_001277"
     CDS             24987..26045
                     /gene="modC"
                     /locus_tag="ACHJWB_001277"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4312936.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26046..26864)
                     /gene="ybhA"
                     /locus_tag="ACHJWB_001278"
     CDS             complement(26046..26864)
                     /gene="ybhA"
                     /locus_tag="ACHJWB_001278"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4312937.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27019..28014
                     /gene="pgl"
                     /locus_tag="ACHJWB_001279"
     CDS             27019..28014
                     /gene="pgl"
                     /locus_tag="ACHJWB_001279"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4312938.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28055..29008)
                     /gene="ybhD"
                     /locus_tag="ACHJWB_001280"
     CDS             complement(28055..29008)
                     /gene="ybhD"
                     /locus_tag="ACHJWB_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4312939.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            29192..30244
                     /gene="ybhH"
                     /locus_tag="ACHJWB_001281"
     CDS             29192..30244
                     /gene="ybhH"
                     /locus_tag="ACHJWB_001281"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4312940.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQCDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30320..31753
                     /gene="ybhI"
                     /locus_tag="ACHJWB_001282"
     CDS             30320..31753
                     /gene="ybhI"
                     /locus_tag="ACHJWB_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4312941.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLMVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVYITLGVWWWNMLIGWNML"
     gene            31936..34152
                     /gene="ybhJ"
                     /locus_tag="ACHJWB_001283"
     CDS             31936..34152
                     /gene="ybhJ"
                     /locus_tag="ACHJWB_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4312942.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMD
                     ARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPN
                     IKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDP
                     GYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGD
                     GSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIP
                     GIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         8219 a         8317 c         9047 g         8596 t
ORIGIN      
        1 atcaaaaaga gataaattgt tctcgatcaa attggctgaa aggcggtaat ttagctataa
       61 attgatcacc gtcgaaaaat gcaaatttgc ttcaacaaaa acctgtttat tgtaaggatt
      121 ttgcggcgta atatatacgt gggatcaatt tgagttttta ttaacatgtt tgcaaccttt
      181 ctttgcgccg tttttgtgtg cattcacatg gtatgatgaa agtgttcaaa caaatttcta
      241 ttggggcatg cgtgtgaccc tttctaacgg ggttcactct cggagtcttc atgcgatgag
      301 caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct tacagtttgc cttgaccgcg
      361 atgtaccact tcctttttgt gccactgacg ctcggtatgg cgttcctgct ggccattatg
      421 gaaacggtct acgtcctctc cggcaaacag atttataaag atatgaccaa gttctggggc
      481 aagttgtttg gtatcaactt cgctctgggt gtggctaccg gtctgaccat ggagttccag
      541 ttcgggacta actggtctta ctattcccac tatgtagggg atatcttcgg tgcgccgctg
      601 gcaatcgaag gtctgatggc cttcttcctc gaatccacct ttgtaggtct gttcttcttc
      661 ggttgggatc gtctgggtaa agttcagcat atgtgtgtca cctggctggt ggcgctcggt
      721 tcaaacctgt ccgcactgtg gattctggtt gcgaacggct ggatgcaaaa cccaatcgcg
      781 tccgatttca actttgaaac tatgcgtatg gagatggtga gcttctccga gctggtgctt
      841 aacccggttg ctcaggtgaa attcgttcac actgtagcgt ctggttatgt gactggcgcg
      901 atgttcatcc tcggtatcag cgcatggtat atgctgaaag gtcgtgactt cgccttcgct
      961 aaacgctcct ttgctatcgc tgccagcttc ggtatggctg ctgttctgtc tgttattgtt
     1021 ctgggtgatg aatccggcta cgaaatgggc gacgtgcaga aaaccaaact ggctgctatt
     1081 gaagccgagt gggaaacgca acctgcgcct gctgccttta ctctgttcgg cattcctgat
     1141 caggaagagg agacgaacaa atttgcgatt cagatccctt atgcactggg catcattgca
     1201 acgcgttccg tggatacccc ggttatcggc ctgaaagagc tgatggtgca gcatgaagaa
     1261 cgcattcgta acgggatgaa ggcgtactct ctgctcgaac aactgcgttc tggttctacc
     1321 gaccaggcgg ttcgtgacca gttcaatagc atgaagaaag acctcggtta cggtctgctg
     1381 ctgaaacgct atacgccaaa cgtggctgat gcgactgaag cgcagattca acaggcaacc
     1441 aaagactcca tcccgcgtgt agcgccgctg tactttgcgt tccgtatcat ggtggcgtgt
     1501 ggcttcctgc tgctggcaat catcgcgctc tctttctgga gtgtcatccg caaccgcatt
     1561 ggcgagaaaa aatggcttct gcgcgccgcg ctgtacggta ttccgctgcc gtggattgct
     1621 gtagaagcgg gctggttcgt ggctgaatat ggccgccaac cgtgggctat cggtgaagtg
     1681 ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg atctcatctt ctcaatggtg
     1741 ctgatttgcg gcctgtatac cctgttcctg gtggcagaat tgttcttaat gttcaaattt
     1801 gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc actttgagca gtcttccacg
     1861 actactcagc cggcacgcta agacaggagt cgtcaaatga tcgattatga agtattgcgt
     1921 tttatctggt ggctgctggt tggcgttctg ctgattggtt ttgcagtcac tgacggtttc
     1981 gacatggggg tgggcatgct cacccgtttc ctcggtcgta acgacaccga gcgtcgaatt
     2041 atgattaact ccattgcacc acactgggac ggtaaccagg tttggctgat caccgcgggc
     2101 ggcgcactct ttgctgcctg gccgatggtc tatgccgctg cgttctccgg cttctatgtg
     2161 gcgatgatcc tcgtgctggc gtctttgttc ttccgtccgg tcggttttga ctaccgctcc
     2221 aagattgaag aaacccgctg gcgtaacatg tgggactggg gcatcttcat tggtagcttc
     2281 gttccgccgc tggtaattgg tgtagcgttc ggtaacctgt tgcagggcgt accgttcaac
     2341 gttgatgaat atctgcgtct gtactacacc ggtaacttct tccagttgct taacccgttc
     2401 ggcctgctgg caggcgtggt gagcgtaggg atgatcatta ctcagggcgc aacctatctg
     2461 caaatgcgta ccgtgggcga actgcacctg cgtacccgtg caacggctca ggtggctgcg
     2521 ctggtgacac tggtctgttt cgcactggct ggcgtatggg tgatgtacgg tatcgatggt
     2581 tatgtcgtga aatcgacaat ggaccattac gcagcctcta acccactgaa taaagaagtg
     2641 gttcgtgaag ctggcgcatg gctggttaac ttcaacaaca cgccaattct gtgggctatt
     2701 ccggcactgg gtgtggttct gccgctgctg accatcctga ctgcacgtat ggataaagcc
     2761 gcgtgggcgt ttgtgttctc ctccctgacg ctggcctgca tcatcctgac agccggtatc
     2821 gcaatgttcc cgtttgtgat gccgtccagc accatgatga acgcaagtct gacaatgtgg
     2881 gatgcaactt ccagccagct gacgcttaac gtcatgacct gggttgcggt ggttctggta
     2941 ccgatcattc tgctctacac cgcctggtgt tactggaaaa tgtttggtcg tatcaccaaa
     3001 gaagatattg aacgtaacac ccactctctg tactaagtaa ggagctaaaa atgtggtatt
     3061 tcgcatggat tctgggaacg cttcttgcct gttcgtttgg ggtaatcacc gcgctggcgc
     3121 ttgaacacgt cgaatcaggc aaagccggtc aagaagacat ctgatgagta agattatcgc
     3181 gactttgtat gcggtaatgg acaagcgccc cctgcgggcg ctttccttcg tgatggcgct
     3241 tctgttagca ggatgtatgt tttgggaccc atctcgtttt gccgcgaaga ccagtgaact
     3301 ggaaatctgg catggtttat tgctgatgtg ggccgtctgt gctggtgtga ttcacggcgt
     3361 gggctttcgt ccgcagaagg ttctttggca agggattttt tgcccattgc ttgccgatat
     3421 tgttctcatt gtcgggctga ttttcttctt cttttaaatc agaattctct ttaaaaaatt
     3481 atgggccgct ccaggcccat aaatttttac gctcccttaa cttgccctca ttcccaaacc
     3541 tcaatcgcgc gcgtatagta gcagcgttta aaagctctaa cttttgttgc attaccggga
     3601 tgtaaagtga atacaacgct gtttcgatgg ccggttcgcg tctactatga agataccgat
     3661 gccggtggtg tggtgtacca cgccagttac gtcgcttttt atgaaagagt acgcacagag
     3721 atgctgcgtc atcatcactt cagtcagcag gcgctgatgg ctgaacgcgt tgcctttgtg
     3781 gtacgtaaaa tgacggtgga atattacgca cctgcgcggc tcgacgatat gctcgaaata
     3841 cagactgaaa taacatcaat gcgtggcacc tctttggttt tcacgcaacg tattgtcaac
     3901 gccgagaata ctttgctgaa tgaagcagag gttctggttg tttgcgttga cccactcaaa
     3961 atgaagcctc gtgcgcttcc caagtctatt gtcgcggagt ttaagcagtg actgacatga
     4021 atatccttga tttgttcctg aaggctagcc ttctggttaa acttatcatg ttgattttga
     4081 ttggtttttc aatcgcatct tgggccatta ttatccagcg gacccgtatt cttaacgcag
     4141 cggcgcgcga agccgaagcg tttgaagata aattctggtc tggaatcgaa ctctctcgcc
     4201 tctatcaaga gagccagggg aaacgggata atctgactgg ttcggaacaa atcttttaca
     4261 gcgggttcaa agagtttgtg cgcctgcatc gtgccaatag ccatgcgccg gaagccgtag
     4321 tggaaggggc gtcgcgtgct atgcgtatct ccatgaaccg tgaacttgaa aatctggaaa
     4381 cgcacattcc gttcctcggt acggttggct ccatcagccc gtatattggt ctgtttggta
     4441 cggtctgggg gatcatgcac gcctttatcg ccctcggggc ggtaaaacaa gcaacactgc
     4501 aaatggttgc gcccggtatc gcagaagcgt tgattgcgac tgcaattggt ctgttcgccg
     4561 caattccggc ggtaatggct tataaccgcc tcaaccagcg cgtaaacaaa ctggaactga
     4621 attacgacaa ctttatggaa gagtttaccg cgattctgca ccgccaggcg tttaccgtta
     4681 gcgagagcaa caaggggtaa gccatggcca gagcgcgtgg acgaggtcgt cgcgaactta
     4741 agtccgaaat caacattgta ccgttgctgg acgtactgct ggtgctgttg ctgatcttca
     4801 tggcgacagc gcccatcatc acccagagcg tggaggtcga tctgccagac gctactgaat
     4861 cacaggcggt gagcagtaac gataatccgc cagtgattgt tgaagtgtct ggtattggtc
     4921 agtacaccgt ggtggttgag aaagatcgcc tggagcgttt accaccagag caggtggtgg
     4981 cggaagtgtc cagccgtttc aaggccaacc cgaaaacggt ctttctgatc ggtggcgcaa
     5041 aagatgtgcc ttacgatgaa ataattaaag cactgaactt gttacatagt gcgggtgtga
     5101 aatcggttgg tttaatgacg cagcctatct aaacatctgc gtttcccttg cttgaaagag
     5161 agcgggtaac aggcgaacag tttttggaaa ccgagagtgt caaaggcaac cgaacaaaac
     5221 gacaagctca agcgggcgat aattatttca gcagtgctgc atgtcatctt atttgcggcg
     5281 ctgatctgga gttcgttcga tgagaatata gaagcttcag ccggaggcgg cggtggttcg
     5341 tccatcgacg ctgtcatggt tgattcaggt gcggtagttg agcagtacaa acgcatgcaa
     5401 agccaggaat caagcgcgaa gcgttctgat gaacagcgca agatgaagga acagcaggct
     5461 gctgaagaac tgcgtgagaa acaagcggct gaacaggaac gcctgaagca acttgagaaa
     5521 gagcggttag cggctcagga gcagaaaaag caggctgaag aagccgcaaa acaggccgag
     5581 ttaaagcaga agcaagctga agaggcggca gcgaaagcgg cggcagatgc taaagcgaag
     5641 gccgaagcag atgctaaagc tgcggaagaa gcagcgaaga aagcggctgc agacgcaaag
     5701 aaaaaagcag aagcagaagc cgccaaagcc gcagccgaag cgcagaaaaa agccgaggca
     5761 gccgctgcgg cactgaagaa gaaagcggaa gcggcagaag cagctgcagc tgaagcaaga
     5821 aagaaagcgg caactgaagc tgctgaaaaa gccaaagcag aagctgagaa gaaagcggct
     5881 gctgaaaagg ctgcagctga taagaaagcg gctgctgaaa aggctgcagc tgataagaaa
     5941 gcggcagcag agaaagctgc agccgacaaa aaagcagcag aaaaagcggc tgctgaaaag
     6001 gcagcagctg ataagaaagc agcggcagaa aaagccgctg cagcaaaagc ggccgcagag
     6061 gcagatgata ttttcggtga gctaagctct ggtaagaatg caccgaaaac ggggggaggg
     6121 gcgaaaggga acaatgcttc gcctgccggg agtggtaata ctaaaaacaa tggcgcatca
     6181 ggggccgata tcaataacta tgccgggcag attaaatctg ctatcgaaag taagttctat
     6241 gacgcatcgt cctatgcagg caaaacctgt acgctgcgca taaaactggc acccgatggt
     6301 atgttactgg atatcaaacc tgaaggtggc gatcccgcac tttgtcaggc tgcgttggca
     6361 gcagctaaac ttgcgaagat cccgaaacca ccaagccagg cagtatatga agtgttcaaa
     6421 aacgcgccat tggacttcaa accgtaatcg cgatgttgac tgttcggacg gtcaacatca
     6481 ggcaccggtt gccacggggt tctggtagtt ttgtgtattt tagtttgtta acattctgct
     6541 aaattatcgt gggccatcgg tccagataag ggagatatga tgaagcaggc attacgagta
     6601 gcatttggtt ttctcatact gtgggcatca gttctgcatg ctgaagtccg cattgtgatc
     6661 gacagcggtg tagattccgg tcgtcctatt ggtgttgttc ctttccagtg ggcggggcct
     6721 ggtgcggcac ctgaagatat tggcggcatc gttgctgctg acttgcgtaa cagcggtaaa
     6781 tttaatccgt tagatcgcgc tcgtctgcca cagcagccgg gtagtgcgca ggaagtacaa
     6841 ccagctgcat ggtccgcact gggcattgac gctgtagttg tcggtcaggt cactccgaat
     6901 ccggatggtt cttacaatgt tgcttatcaa cttgttgaca ctggcggcgc accgggtact
     6961 gtacttgctc agaactcgta caaagtgaac aagcagtggc tgcgttatgc tggtcatacc
     7021 gccagtgatg aagtgtttga aaaactgacc ggcattaaag gtgcgttccg tacccgtatt
     7081 gcctacgttg ttcagaccaa cggcggtcag ttcccgtatg aactgcgcgt atctgactat
     7141 gacggttaca accagtttgt cgttcaccgt tcaccgcagc cgctgatgtc tccggcgtgg
     7201 tcaccagacg gttctaaact ggcttatgtg accttcgaaa gcggtcgttc cgcgctggtt
     7261 attcagacgc tggcaaatgg cgctgtacgt caggtggctt cattcccgcg tcacaacggt
     7321 gcacctgcat tctcgccaga cggcagcaaa ctggcattcg ccttgtcgaa aaccggtagt
     7381 ctgaacctgt acgtaatgga tttggcttct ggtcagatcc gccaggtgac tgatggtcgc
     7441 agtaacaata ccgaaccgac ctggttcccg gatagccaga acctggcatt tacttctgac
     7501 caggccggtc gtccgcaggt ttataaagtg aatatcaacg gcggtgcgcc acaacgtatt
     7561 acctgggaag gttcgcagaa ccaggatgcg gatgtcagca gcgacggtaa atttatggta
     7621 atggtcagct ccaatggtgg gcagcagcac attgccaaac aagatctggc aacgggaggc
     7681 gtacaagttc tgtcgtccac gttcctggat gaaacgccaa gtctggcacc taacggcact
     7741 atggtaatct acagctcttc tcaggggatg ggatccgtgc tgaatttggt ttctacagat
     7801 gggcgtttca aagcgcgtct tccggcaact gatggacagg tcaaattccc tgcctggtcg
     7861 ccgtatctgt gataataatt aattgaatag taaaggaatc attgaaatgc aactgaacaa
     7921 agtgctgaaa gggctgatga ttgctctgcc tgttatggca attgcggcat gttcttccaa
     7981 caagaacgcc agcaatgacg gcagcgaagg catgctgggt gccggcactg gtatggatgc
     8041 gaacggcggc aacggcaaca tgtcttccga agagcaggct cgtctgcaaa tgcaacagct
     8101 gcagcagaac aacatcgttt acttcgatct ggacaagtac gatatccgtt ctgacttcgc
     8161 tcaaatgctg gatgcacatg caaacttcct gcgtagcaac ccgtcttaca aagtcaccgt
     8221 agaaggtcac gcggacgaac gtggtactcc ggaatacaac atctccctgg gtgaacgtcg
     8281 tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt tctgcagacc agatctccat
     8341 cgtttcttac ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg catactccaa
     8401 aaaccgtcgt gcggtactgg tttactaaga gaattgcatg agcagtaact tcagacatca
     8461 actattgagt ctgtcgttac tggttggtat agcggccccc tgggccgctt ttgctcaggc
     8521 accaatcagt agtgtcggct caggctcggt cgaagaccgc gtcactcaac ttgagcgtat
     8581 ttctaatgct cacagccagc ttttaaccca actccagcaa caactttctg ataatcaatc
     8641 cgatattgat tccctgcgtg gtcagattca ggaaaatcag tatcaactga atcaggtcgt
     8701 ggagcggcaa aagcagatcc tgttgcagat cgacagcctc agcagcggtg gtgcagcggc
     8761 gcaatcaacc agcagcgatc aaagcggtgc ggcggcatca acgacgccga cagctgatgc
     8821 tggtactgcg aatgctggcg cgccggtgaa aagcggtgat gcaaacacgg attacaatgc
     8881 agctattgcg ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg catttcagaa
     8941 tttcatcaaa aattaccctg attcaactta cctgccaaac gccaattatt ggctgggtca
     9001 gttaaactac aacaagggta aaaaagatga tgcggcgtac tattttgctt cggtagtgaa
     9061 aaactatccg aagtcaccaa aggctgctga tgcgatgttt aaagtcggcg tcatcatgca
     9121 ggacaaaggt gacaccgcaa aagcgaaagc cgtataccag caggttatca gtaaataccc
     9181 tggtactgat ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat gcataacaca
     9241 cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt tgagtgatct
     9301 gcatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc cgttgccacg
     9361 ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt ggtagagcag
     9421 ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccag ttttagcatc
     9481 gaagacagat gttaagcgtg taggataacg ttgcgtcagc aacggcccgt agggcgagcg
     9541 aagcgagtca tcctgcacga cccaccacta attacggtgg gttcggtgga agtagtttgt
     9601 agtatccagc gcagtatcgg gtgattagct cagctgggag agcacctccc ttacaaggag
     9661 ggggtcggcg gttcgatccc gtcatcaccc accactcggg tcgttagctc agttggtaga
     9721 gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaattttaa
     9781 catcaaactc agatgttaag cgtgaaggat aacgttgcgc cagcaacggc ccgtagggca
     9841 agcgaagcga gtcatcctgc acgacacacc aatcttaaag attggccccg agtagaaatc
     9901 tttcaggtaa cacccgtatg ggtcgttagc tcagttggta gagcagttga cttttaatca
     9961 attggtcgca ggttcgaatc ctgcacgacc caccatcctg aatgattaaa gcagcataat
    10021 cccgcaaggg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
    10081 gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat
    10141 ctgcgatact caaagattcg aacctgcagc aggtttgagt tgagcgcagc gaaacaacgg
    10201 agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca cgacccacca
    10261 atgtaaaaaa gcgccctaaa ggcgcttttt tgctattcag gcatcctcaa tttcactttg
    10321 taaacctgat gacatcgtca gagcttactg tgcaagcaac tctacgtcgg tggaattagg
    10381 cgtaattcga gtgacttttc cccaccattc gactatcttg tttagcatat aaaacaaatt
    10441 acaccgataa cagcgaatat tacgctaatg tcggttttaa cgttaagcct gtaaaacgag
    10501 atggtaagat gagcgtaatg tttgatccag acacggcgat ttatcctttc cccccgaagc
    10561 cgacgccgtt aagcattgat gaaaaagcgt attaccgcga gaagataaaa cgtctgctaa
    10621 aagaacgtaa tgcggtgatg gttgcccact actataccga tcccgaaatt caacaactgg
    10681 cagaagaaac cggtggctgt atttctgatt ctctggaaat ggcgcgcttc ggtgcaaagc
    10741 atcccgcttc tactttgtta gtcgctgggg tgagatttat gggagaaacc gccaaaattc
    10801 tcagtccgga aaaaacaatt ctgatgccga cacttcaggc tgaatgttca ctggatctcg
    10861 gctgccctgt tgaagaattt aacgcatttt gcgatgccca tcccgatcgt actgtcgtcg
    10921 tctacgccaa cacttctgct gcggtaaaag cgcgcgcaga ttgggtggta acttcaagca
    10981 tttccgtcga acttattgat catcttgata gtttgggtga aaaaatcatc tgggcacccg
    11041 acaaacatct ggggcgttac gtgcaaaaac agacgggtgg agacattcta tgctggcagg
    11101 gtgcctgtat tgtgcatgat gaatttaaga ctcaggcgtt aacccgcttg caagaagaat
    11161 acccggatgc tgccatactg gtgcatccag aatcaccaca agctattgtc gatatggcgg
    11221 atgcggtcgg ttccaccagt caactgatcg ctgctgcgaa aacattgcca catcagcggc
    11281 ttatcgtggc aaccgatcgg ggcattttct acaaaatgca gcaggcagtg ccagataaag
    11341 agttactgga agcaccaacc gcaggtgagg gtgcaacctg ccgcagctgc gcgcattgtc
    11401 cgtggatggc catgaatggc cttcagtcca tcgcagaggc attagaacag gaaggaagca
    11461 atcacgaggt tcatgttgat gaaaggctgc gagagagggc gctggtgccg ctcaatcgta
    11521 tgctggattt tgcggctaca ctacgtggat aacgaataat aaggcgtaac gttacgcttt
    11581 gggggaaaga tggatttttt tagtgtgcag aatatcctgg tacatatacc aataggggca
    11641 ggcggttatg atctctcatg gatcgaagcg gtaggcacga tcgccgggtt gctgtgtatt
    11701 ggccttgcca gtctggagaa gatcagcaac tacttctttg gcctgatcaa cgtcaccttg
    11761 tttggcatta ttttctttca gattcagctg tatgccagcc tgctattaca ggtgtttttc
    11821 tttgccgcga atatttacgg ttggtatgcg tggtcgcgac aaaccagtca gaacgaggcg
    11881 gagttgaaaa ttcgctggtt gccattgccg aaggcactca gctggttggc ggtttgcgtt
    11941 gtttcgattg gtctgatgac ggtatttatc aatccggtgt ttgcattttt gacccgcgtg
    12001 gcagtcatga tcatgcaagc attaggatta caggttgtga tgcctgaact gcaaccggac
    12061 gctttcccgt tctgggattc atgcatgatg gtgttatcta tcgtggcaat gattctgatg
    12121 acgcgtaagt atgtggaaaa ctggctgttg tgggtgatta ttaacgtgat tagcgtcgtt
    12181 atttttgcac ttcagggcgt ttacgccatg tctctggagt acatcatcct gacctttatt
    12241 gcgctcaacg gcagccggat gtggatcaac agcgcacgtg aaagaggctc acgcgcgctg
    12301 tcccattaat ggtgatgatg tgaatggcca gacactcctt cattcaggtg gcagtctggc
    12361 ccatgacaag gttgatattc catctgaatg gtggcgtgct caatctgata gtgatccatc
    12421 aggtagtgtt ggatctgatc caacaaggca tcgtgatcgt gtggcgggat cacctgcaca
    12481 tgcaacgtca tcaccggctt ctcgcctacc atccatacat gtacatggtg cacattgcgg
    12541 acttccggga tttcccggca catacgacgc ttcagttcag cgatatccag cgataccggt
    12601 gcaccttcaa gtaattcatt cacgctatct ttcaacagtc gccacgcgct acgcagaacc
    12661 agaagcgaca ccagtatcga gagaatgggg tcagcaggtg tccagccagt ccagataata
    12721 atcaacgcgg caataatagc cccaaccgaa cccagcaggt ccccgagcac atgcagtgcc
    12781 gctgctcgta cgttgaggtt tttctcttcg ctgccgtgat gaagtaacca gaaagaaagt
    12841 atatttgcca gcaacccagc cacggcaatt gccatcatca tgccgccctc gaccggacgc
    12901 ggcgtgcgga agcgttctat cgcctcccag acaatcaaaa tggtaattac cacgagggct
    12961 atggcattca caaacgccgc gagcgtggtc agtcttagcc aaccgaaagt gtggcgaatg
    13021 gtgggaggac gacgtgaaaa ttgcacggcg agtagggcga aaagcagggc ggcagtatcg
    13081 gtcaacatat gacccgcatc ggccagcaat gccagagaac cagaaagaaa accaccaacg
    13141 acttctacca gcataaaccc ggcagtcacg ccgaaagcat acaacaagcg acgagcatta
    13201 ttatcttctg gcaggtgtga ggacgtgtgt gagtgtgagt gcgccatgac gagtcatccc
    13261 ttaattattc tcacttaact ttatgacatc atactgcttt tagaagtgaa aaattaaaag
    13321 ggagagactc cgctctccca ttattggcta ttttgcaggg ttactgcgtg gtactgtcgg
    13381 ttttggtatc gacatcatta ttgatgccat caccggtttg taccttttta ttgatatccg
    13441 gacagcgacc atctttgcac atggtgttct tgtgctcttc atctttggtc attccgtcat
    13501 tgttcattga agaaccatcc gaatgcagca ttgtgccacc agaaccggta tttaccccgt
    13561 tattgtcgac gttatttggc gcgacatttt cacgggcgtc aggggctacc tggcccgcat
    13621 cagctgcggc gtttgcctgg ccgttattag tttgcgctcc gctatcggca gccagtgcgg
    13681 caccgctggc aaggcttaga gtggcagtca gaaataatgt ggccagtttt gtcattttca
    13741 taggatgctc ctgttatggt cgttatgtcg gataacctct tccaacagtg catttgcagg
    13801 tgaatataag gcattggttt aagatttcag ccaggttatg aaacgcagca gagaatcttg
    13861 aaataattaa caaacaaagg agttacagtt agaaattgta ggagagatct cgtttttcgc
    13921 gacaatctgg cgtttttctt gctaattcca ggattaatcc gttcatagtg taaaaccccg
    13981 tttacacatt ctgacggaag atatagattg gaagtattgc attcactaag ataagtatgg
    14041 caacactgga acagacatga attatcagaa cgacgattta cgcatcaaag aaatcaaaga
    14101 gttacttcct cctgtcgcat tgctggaaaa attccccgct actgaaaatg ccgcgaatac
    14161 ggttgcccat gcccgaaaag cgatccataa gatcctgaaa ggtaatgatg atcgcctgtt
    14221 ggtggtgatt ggcccatgct caattcatga tcctgtcgca gcaaaagagt atgccactcg
    14281 cttgctggcg ctgcgtgaag agctgaaaga tgagctggaa atcgtaatgc gcgtctattt
    14341 tgaaaagccg cgtaccacgg tgggctggaa agggctgatt aacgatccgc atatggataa
    14401 cagcttccag atcaacgacg gtctgcgtat agcccgtaaa ttgctgcttg atattaacga
    14461 cagcggtctg ccagcggcgg gtgagtttct cgatatgatc accccacaat atctcgctga
    14521 cctgatgagc tggggcgcaa ttggcgcacg taccaccgaa tcgcaggtgc accgcgaact
    14581 ggcatcaggg ctttcttgtc cggtcggctt caaaaatggc accgacggta cgattaaagt
    14641 ggctatcgat gctattaatg ccgccggtgc gccgcactgc ttcctgtccg taacgaaatg
    14701 ggggcattcg gcgattgtga ataccagcgg taacggtgat tgccatatca ttctgcgcgg
    14761 cggtaaagag cctaactaca gcgcgaagca cgttgctgaa gtgaaagaag ggctgaacaa
    14821 agcaggcctg ccagcacagg tgatgatcga tttcagccat gctaactcgt ccaaacaatt
    14881 caaaaagcag atggatgttt gtgctgacgt ttgccagcag attgccggtg gcgaaaaggc
    14941 cattattggc gtgatggtgg aaagccatct ggtggaaggc aatcagagcc tcgagagcgg
    15001 ggagccgctg gcctacggta agagcatcac cgatgcctgc attggctggg aagataccga
    15061 tgctctgtta cgtcaactgg cgaatgcagt aaaagcgcgt cgcgggtaag gtttaattat
    15121 cggatgcgcc gtcagagtgg cgtatccgat gaatcaccac aggcctgata agacgcgcag
    15181 cgtcgcatca ggcaatgtgc tccattgtta gcaacgacaa acaaaaaagc cgactcactt
    15241 gcagtcggct ttctcatttt ttaaacgaat gacgtttact tcgctttacc ctggttcgca
    15301 acggctgctg ctttcgccgc gatctcgtca gcattaccca gataatagcg tttcagcggt
    15361 ttgaaattct cgtcgaactc atacaccagc ggcacgccag tcgggatatt cagctcaaga
    15421 atctcttctt cgctcatgtt atcaagatat ttcaccagcg cacgcaggga gttaccgtgt
    15481 gcggcgatga ttacgcgctc accgctcttc atacgcggca gaatggtttc attccagtaa
    15541 gggatcacgc ggtcaatggt cagcgccagg ctttccgtca gcggcaattc tttctcgctc
    15601 agtttcgcgt aacgcggatc gtgacccgga taacgctcat catctttagt cagttccggc
    15661 ggagtcactg caaaaccacg acgccactgt ttcacctgct cgtcgccata cttttcagca
    15721 gtttccgctt tgttcagacc ctgcaacgca ccgtagtgac gttcgttcag tttccaggat
    15781 ttctcaacgg gcagccatgc ctgatccagt tcgtccagca cattccacag ggtatggata
    15841 gcgcgtttca gcacagaagt gtaagcaaag tcaaagctgt aaccttcctc tttcagcagc
    15901 ttacctgctg cttttgcttc gcttacgcct ttctcagaca gatccacgtc gtaccaaccg
    15961 gtgaaacggt tttctttgtt ccactgactt tcgccatgac gaaccagaac cagcttagtt
    16021 acagccatat acttactcct caaatcatct tttaatgata ataattctca ttatattgcc
    16081 gtgatgaagc aacagcaatg cttacgcata accatagcga aaatagtggc gcagtgtaag
    16141 gttgttgtga atattgagtt gcaaatatgt cggtgtttgc tggtgatttg aacaatatga
    16201 agataaagcc ctcatgacga gggcttaaca ttactcagca ataaactgat attccgtcag
    16261 gctggaatac tcttcgccag gacgcaggaa gcagtccggt tgcggccatt cagggtggtt
    16321 cgggctgtcc ggcagaaact cgctttccaa cgccagccct tgccagtcgg cgtaaggttc
    16381 ggttccccgc gacggtgtgc cgccgaggaa gttgccggag tagaattgaa gagccggagc
    16441 ggtggtgtag accttcagct gcaatttttc atctgctgac cagacatgcg ccgccacttt
    16501 cttgccatcg cctttggcct gtaacaagaa tgcgtgatcg taacctttca ctttgcgctg
    16561 atcgtcgtcg gcaagaaact cactggcgat gattttggcg ctgcggaaat caaaagacgt
    16621 tccggcgaca gatttcaagc cgtcgtgcgg aatgccgcct tcatcaaccg gaagatattc
    16681 gtccgccaga atctgcaact tgtgattgcg cacgtcagac tgctcgccgt caagattgaa
    16741 atagacgtga ttagtcatat tcaccgggca aggtttatca actgtggcgc gataagtaat
    16801 ggagatacgg ttatcgtcgg tcagacgata ttgcaccgtt gcgccgagat tacccgggaa
    16861 gccctgatcg ccatcatctg aactcagggc aaacagcacc tgacgatcgt tctggttcac
    16921 aatctgccag cgacgtttgt cgaacccttc cgggccgccg tgcagctggt taacgccctg
    16981 acttggcgaa agggtcacgg tttcaccgtc aaaggtataa cggctattgg cgatgcggtt
    17041 ggcataacga ccaatagagg cccccagaaa cgcggcctga tcctgatagc attccgggct
    17101 ggcacagccg agcagcgcct cgcggacgct gccatcggaa agcggaatac gggctgaaag
    17161 taaagtcgca ccccagtcca tcagcgtgac taccatccct gcgttgttac gcaaagttaa
    17221 cagtcggtac ggctgaccat cgggtgccag tgcgggagtt tcgttcagca ctgtcctgct
    17281 ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac ctgtttttgc ttcatattgt
    17341 tcagcgacag cttgctgtac ggcaggcacc agctcttccg ggatcaacgc gacgatacag
    17401 ccgccaaatc cgccgccggt catgcgtacg ccacctttgt cgccaatcac agctttgacg
    17461 atttctacca gagtgtcaat ttgcggcacg gtgatttcga aatcatcgcg catagaggca
    17521 tgagactcct ccatcaactc gcccatacgt ttcaggtcgc cctgctccag cgcgctggca
    17581 gcttcaacgg tgcgggcgtt ttcagtcagg atatgccgca cgcgtttcgc cacgattggg
    17641 tccagctcat gtgcaacagc gttgaactct tcaatggtga catcgcgcag ggctggctgc
    17701 tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg tgttgtattc gctgccaacc
    17761 agggtacgtt tgaagttact gttgatgatg acgacagcca cacctttcgg catggaaact
    17821 gctttggtcc ccagtgagcg acagtcaatc agcaaggcat gatctttctt gccgagtgcg
    17881 gaaattagct gatccatgat cccgcagtta cagccaacaa actggttttc tgcttcctga
    17941 ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat aaagctgctg caatacggtt
    18001 ccgaccgcga cttccagtga agcggaagaa cttaacccgg caccctgcgg cacattgccg
    18061 ctgatcacca tgtccacacc gccgaagctg ttgttacgca gttgcagatg tttcaccacg
    18121 ccacgaacgt agttcgccca ttgatagttt tcatgcgcga caatgggcgc attgagggaa
    18181 aactcgtcga gctgattttc ataatcggct gccatcacgc gaactttacg gtcatcgcgt
    18241 ggtgcacagc tgatcacggt ttgataatca atcgcgcagg gcagaacgaa accgtcgttg
    18301 tagtccgtgt gttcaccaat caaattcacg cggccaggcg cctgaatggt gtgagtggca
    18361 gggtagccaa atgcgttggc aaacagagat tgtgtttttt ctttcagact catttcttac
    18421 actccggatt cgcgaaaatg gatatcgctg actgcgcgca aacgctctgc tgcctgttct
    18481 gcggtcaggt ctcgctgggt ctctgccagc atttcataac caaccataaa tttacgtacg
    18541 gtggcggagc gcagcagagg cggataaaag tgcgcgtgca gctgccagtg ttgattctct
    18601 tcgccattaa atggtgcgcc gtgccagccc atagagtagg ggaaggagca ctggaagagg
    18661 ttgtcataac gactggtcag ctttttcaac gccagcgcca gatcgctgcg ctgggcgtcg
    18721 gtcaaatcgg tgatccgtaa aacgtgggct ttgggcagca gtagcgtttc gaacggccag
    18781 gcagcccagt aaggcacaac ggctaaccag tgttcggttt cgacaacggt acggctaccg
    18841 tctgccagct cgcgctgaac ataatccacc agcattggtg atttctgttc ggcaaaatat
    18901 tctttttgca ggcggtcttc gcgctcagct tcgttaggca ggaagctatt tgcccaaatc
    18961 tgtccgtgcg gatgcgggtt agagcagccc atcgccgcac ctttgttttc aaagacctgc
    19021 acccacgggt acgtttttcc cagctctgcg gtttgctcct gccaggtttt gacgatttcc
    19081 gtcaatgccg caacgctcag ttctggcaac gttttactgt gatccggtga aaagcagatc
    19141 acccggctgg taccgcgcgc gctctggcaa cgcatcaaag gatcgttgct ttctggcgca
    19201 tctggcgtgt cagacatcaa ggccgcaaag tcattagtga aaacgtaagt cccggtgtaa
    19261 tcggggtttt tatcgcctgt cacccgcaca ttacctgcgc agaggaagca atctggatcg
    19321 tgcgcaggta acacctgttt ggctggcgtt tcctgcgccc cctgccaggg gcgcttagcg
    19381 cggtgcggtg aaaccagaat ccattgcccg gtgagcgggt tgtagcggcg atgtggatga
    19441 tcaacgggat taaattgcgt catggtcgtt ccttaatcgg gatatccctg tggatggcgt
    19501 gactgccagt gccaggtgtc ctgcgccatt tcatcgagtg tgcgcgttac gcgccagttc
    19561 agttcacggt cggctttgct ggcgtccgcc cagtaggccg gaaggtcgcc ctcgcgacgc
    19621 ggtgcaaaat gataattaac cggtttgccg caggctttgc tgaaggcatt aaccacgtcc
    19681 agcacgctgt tgcctacgcc agcgccgagg ttgtagatgt gtacgcctgg cttgttcgcc
    19741 agtttttcca tcgccacgac gtgaccgtcc gccagatcca ttacgtggat gtaatcgcgt
    19801 acgccagtac catcttcggt cggataatcg ttaccaaaaa tcgccagcga gtcgcgacgg
    19861 cctacagcaa cctgggcgat gtatggcatc aggttattcg gaatgccttg cggatcttcg
    19921 cccatatcgc ccgacggatg cgcgccaacc gggttgaagt agcgcagcag ggcaatgctc
    19981 cagtccggct gggctttttg cagatcggtg aggatctgtt ccaccatcag cttgcttttg
    20041 ccgtaagggc tttgcggtgt gccggtcggg aagctttcaa cgtatggaat tttgggctga
    20101 tcgccataaa cggtggcgga ggagctaaaa ataaagtttt tgacgttagc ggcgcgcatg
    20161 gcgctaatca ggcgcagagt gccgttgaca ttgttgtcgt aatattccag cggtttttgt
    20221 accgattcgc caacggcttt cagcccggcg aagtggatca cggtgtcgat agcgtgatcg
    20281 tgcagtatct cggtcatcaa cgcttcgtta cgaatatcgc cttcaacaaa cgttggatgt
    20341 ttgccgccta aacgctcgat aacaggcagt acgctgcgct tactgttaca gaggttatca
    20401 agaatgatga catcatgacc gttttgcagt aattgcacgc aggtatgact tccaatgtaa
    20461 ccgctaccac cggtaaccag aactctcata attcgctcca ttaggcttat ggtatgaaat
    20521 aaccatagca taacaaagat gcgaaaagtg tgacatggaa taaattagtg gaatcgttta
    20581 cacaagaatt tagccgtttt ttatgcgcga ttaagtgatt ataaaacaga gggtttatga
    20641 atgattgcgc tttttatctg aaaaaagaag cggtttcatg cctgcatgcg tcgaaccgtt
    20701 ggccggagag ggcgcttagg ctgcctccgg caaggtcagc actaccgact caatatattt
    20761 ttgtcagcac atagcgatag agtccaccgt ccggcacgaa ctcaaggcga tgggtaatac
    20821 aggcaggcgc atcttcagcg tggtgcgaaa caaacaacaa ttgcgtttca ccttcgctaa
    20881 tcagcacatc aacaaaacgg cggataagct ggcgattgag cggatcaagc ccctgtagtg
    20941 gttcatcgag aataagcaac gtcggatgtt tcaccagcgc gcggacgatc agcgccagac
    21001 gctgctgtcc ccaggaaaga ctatggaacg gagcgtcagc cgtgcgttta tcaatgccga
    21061 gaatatccag ccactgctgc accagttttt gctggcgatc cgaaacggcc tgataaatgc
    21121 caatcgaatc aaaatagcca gaaagaatca cattacgcac ggtagtgctg acccggtaat
    21181 ccagatgcaa actactgctg acgtaaccga tatgcttttt gatatcccag atggtttcgc
    21241 cgctgccgcg acgtcgtccg aaaagcgtca aatcgttgct gtaaccttgc ggatgatcgc
    21301 cagtaaccag gcttaataac gtcgattttc ctgcaccatt tggcccgaca atttgccagt
    21361 gttcacctgg attcacctgc cagctaaggt tattaagaat ggggcgatcg ttataagaaa
    21421 ccacgccatt gttcagcaca atgcgcggtt cgttggcggg taaggcgtga cgtgctgaag
    21481 gttcatccgg ctccggcagt tgcacacctt caagctgttc actatgcgcc agttgcgcga
    21541 cgagtgcttg ttggagcagt tcctctttag cgccagtttc cgctaacgtg caatccgtca
    21601 gcacgccagc aaactggaca aactccggga tctcatcgaa gcgattgagc accagtacca
    21661 gagtaatacc ggactgatgt aacgaggcga gtcgctcagc cagctgctga cgtgaggcaa
    21721 catccaggcc atcgaacggc tcatcaagaa tcaacaagtc aggctccgac atcagcgcct
    21781 gacacagcag ggtttttcgc gtctcgccag tggaaaggta tttaaagcgt cggtcgagga
    21841 gggcggtaat accgaactgc tgcgccagtt gcatgcaacg cggtgcatcc tttacttcat
    21901 cctgaatgat ctcagccgta gtgcgtccgg tgtcatcttc gccagggccg agcatatcgg
    21961 tgttattccg ctgccattcg tcgctgacga gtttttgcaa ttgctcgaag gagagacgag
    22021 tgatgtggga aaactggctt tgccgttcac ctttcaaaag cggaagttcc cccgccagcg
    22081 cgcgggccag ggccgatttc ccgcttccat tcgaaccgac aaacgcccaa ctatcacccg
    22141 cgtttaacgt tagctgaggc aattgcagcg tttttgtgtc gctaagacga aacgtgcctt
    22201 gcaaaatttg caacgatgac attttatatc ccattttgtg cagcgattac tgacagggat
    22261 acgtgtttca taacaaattg tcaacacgct tagcacagcg tggcgataat cacgctgtcg
    22321 gcattaaagt aggccgtgac attctgccct tgctgaagag aagtcgcttc atttaccggc
    22381 actgtggcgc acagtgtttg cccgtcgggt agcgccatta atacttcgca ctgctctgcg
    22441 ccgcgctcaa tatgactaat aatacccggt aattggttgt cggcgttttg tgcgaccgct
    22501 tcgtcctgag taatgcctac ccacggcgct tttagcaata tcaacacttc tttgccttca
    22561 tccagcccca gacgtgcgcc gctttgtgcg gtaattgcga ctttcaggcg tgtttttccg
    22621 tcagccagta agacatcaac atgctgttga acgtcatcat gatcgcgggc ggtgatggta
    22681 ccgaaccact ggttacgggc gctggtttgc agtgaaaaac gtgagatcgc ggccagcagg
    22741 ctgttcagcg gcagggcgtc atcgtcactt aacacatcaa aggctttttg ctggatttgc
    22801 gccagtaagt catagagctg aatcagtcgc tgaccatagc gggtcagtac tgcgccgccg
    22861 ccacctttac cgcctgttgc gcgctcgacc agaatatgct cacttaactg attcatctcg
    22921 ttaatggcat cccaggcgct tttatagctg ataccggcat ctttcgctcc ctggctaatg
    22981 gaaccggaaa gcgcaatgtg ttttagtagc gaaatccggc gcgggtcggc gaataatttt
    23041 tgttggagct taagggtgag aaggatttcg gcctgcataa caatgtcctg gcaaaagtct
    23101 tattgtgacg gaaaacgaac gccacgcaaa gctgaccgca caaaagggga gtgcttttct
    23161 gtgcttagcg gttagaatag tctcatgact atatctggag ttgaccatgt tagagttatt
    23221 aaaaagtctg gtattcgccg taatcatggt acctgtcgtg atggccatca tcctgggtct
    23281 gatttacggt cttggtgaag tattcaacat cttttctggt gttggtaaaa aagaccagcc
    23341 cggacaaaat cattgattcc ctgaatgccc gcttagtcgg gcatttcttt ttctcaactt
    23401 cctgcttttc cagccgatat tttttcttat ctacctcaca aaggttagca ataactgctg
    23461 ggaaaattct gagctagccg ttatattgtc acctatataa cgttacatta aggggttacc
    23521 aatggctcgt aaatggttga acttgtttgc cggggcggca ctctctttcg ctgttgctgg
    23581 caatacactg gcagatgaag ggaaaatcac ggtgttcgcc gccgcatcac tgactaacgc
    23641 aatgcaggac attgctacgc agtataaaaa agaaaaaggc gtggatgtgg tttcttcttt
    23701 cgcttcgtca tctactttgg cccgtcagat tgaagcgggt gcgcctgcgg atctgtttat
    23761 ttctgccgat cagaaatgga tggattatgc ggttgataaa aaagcgatcg atacggcttc
    23821 gcgccagaca ctgctcggca atagcctggt cgttgtagca ccgaaagcca gcgagcagaa
    23881 agatttcacc atcgacagca aaaccaactg gacttcgctg ctgaatggcg gtcgcctcgc
    23941 ggttggcgat ccggaacatg tccctgctgg catttatgcg aaagaagcac tgcaaaaact
    24001 gggcgcatgg gatacgctct ctccgaaact ggctccggcg gaagatgttc gtggcgcgct
    24061 ggcgttggtc gaacgcaatg aagcgccgtt gggcattgtt tacggttctg acgcagttgc
    24121 cagcaaaggg gtaaaagtgg ttgccacctt cccggaagat tcacataaaa aagtggaata
    24181 tccggttgct gttgtggaag ggcataacaa tgcgacagtg aaagcatttt atgattattt
    24241 gaaagggccg caggctgcgg aaatctttaa acgttacgga tttacaacca agtaatgata
    24301 ctgaccgatc cagaatggca ggcagtttta ttaagcctga aagtttcttc cctggctgtg
    24361 ctgtttagcc tgccgtttgg gatctttttt gcctggttac tggtgcgttg cacgtttccg
    24421 ggcaaagctc tgctcgacag cgtactgcat ctaccgctgg tgttaccgcc cgtggtcgtc
    24481 ggttacttat tattagtttc gatgggacgg cgcggattta tcggtgaacg tctgtatgac
    24541 tggtttggta ttaccttcgc ctttagctgg cgcggcgcgg ttctcgctgc cgccgtcatg
    24601 tcgtttccgc tgatggtgcg ggcaattcgt ctggagctgg aaggggttga tgtcaaactc
    24661 gaacaggccg caagaacact gggggccggg cgctggcgcg ttttctttac tatcacgtta
    24721 ccgctgacct taccgggaat tattgttggt acggtactgg cttttgctcg ttctctcggt
    24781 gagtttggtg caaccatcac ctttgtgtcg aacattcctg gtgaaacgcg aaccattcct
    24841 tctgccatgt ataccctgat ccagaccccc ggcggggaaa gtggcgcggc gagactgtgc
    24901 attatttcta ttgcgctggc gatgatctcc ctgttgattt cagaatggct ggccagaatc
    24961 agccgtgaac gggcggggcg ctaatcatgc tggaactgaa tttttcccag accttgggca
    25021 accattgcct gaccattaat gaaacgctgc ccgccaatgg catcactgct atctttggcg
    25081 tctccggtgc cggaaaaact tcgctgatta acgccatcag tggactgacg cgcccgcaaa
    25141 aagggaggat tgtcctcaat ggtcgggtac taaatgatgc cgaaaaaggt atctgcctga
    25201 cgccagaaaa gcgtcgcgtt ggctatgttt ttcaggatgc gcggctgttt ccgcattaca
    25261 aagtgcgtgg caatctgcgc tacggcatgg cgaaaagcat ggtcgatcag ttcgataagc
    25321 tggtggcgct tttaggcatt gaaccgttgc ttgaccgttt accaggcagc ctgtccggag
    25381 gcgaaaaaca gcgcgtagcg attgggcggg ctttgctgac ggcaccagaa ttgctgttgc
    25441 tggatgaacc gctggcgtca ctggatattc cgcgtaaacg cgaactgttg ccttatctgc
    25501 aacgtctgac gcgggaaatc aacatcccga tgttgtatgt cagccattcg ctggatgaga
    25561 tcctccatct ggcagacaga gtgatggtac tggaaaacgg tcaggtgaaa gcctttggcg
    25621 cactggaaga agtctggggt agtagcgtga tgaatccgtg gctgccgaaa gagcaacaaa
    25681 gtagcattct gaaagtgacg gtacttgagc atcatccgca ttacgcgatg actgcgctgg
    25741 cgctgggcga tcagcatttg tgggtcaata agctggacga accgctgcaa gctgcgctac
    25801 gtatccgcat tcaggcttcc gatgtttctc tggttttaca accgccgcag caaaccagca
    25861 ttcgtaacgt attgcgggca aaagttgtta atagttatga cgacaacggc caggtggaag
    25921 tggaactgga agtcggaggt aaaacgctgt gggcgcgtat cagcccgtgg gccagggatg
    25981 aactggtgat caaacctggc ctgtggctgt acgcgcaaat taaaagtgtg tcgataaccg
    26041 cctgattaaa tcaggtggct ataaatgaac tgggcaatgc tgtcggtggt gttatcacca
    26101 atcacaatgt tggcgcgcgc ttttaccgcg tcatcggcgt tgcccatcgc cacgcctgta
    26161 ccagcggcct ccagcatact gatatcatta aagttatcgc cgaacgccac gacgttttcc
    26221 atcgaccagc cttgcgcctc aacccatttc gtcaaacgtt tacctttgct gttgccgccg
    26281 cgtgcaatat caacctgatc gtgccaggac cattcacact ccagtcccag ttcatgttcg
    26341 acatgcttac caaaatgctg caattgcggc aggtcatcgt gcgtcagggc gaacttccat
    26401 acggcgttaa cttgttgcgc cgtttcagcc agagaagcga cttgtgtgaa agtcggacgc
    26461 tgttccggcg gcagggtttg cgcccagtta gacgtgcgaa tgacatgccc ggtcgggtgc
    26521 tcatagacca ttgcatcatc cacatacatc agaccgtgaa tgtggtgttc attcagcatc
    26581 tcaatgagtt gcagggcttt attaacgggc attgggtccg cttccagcac ggtttttgca
    26641 tgataatcat acaaataggt gccattacag caaatagcag gtgtatccag cgccagcgcc
    26701 tgataaaaag gatgaatagc gacgtgatgg cgacctgtga cgatgattaa ttgatagcct
    26761 gcttcgcgag cgcgggccag ggcttctatc gatgaaggaa gcagggtctt tttcggggtc
    26821 aataaggtgc cgtctaagtc gagagcaatc acgcgtgtgg tcatggcgta ttccagatta
    26881 aggttaagaa ttttcgtctg cgcgaatggt ataccgatac cactatcgga caaaattctg
    26941 cgttttaatt cagcattcac cgccaaaagc gactaatttt agctgttaca gtcagttgct
    27001 aaatgcaaag gagcattcat gaagcaaaca gtttatatcg ccagccctga gagccagcaa
    27061 attcacgtct ggaacctgaa tcatgaaggc gcactgacgc tgacacaggt tgtcgatgtg
    27121 ccggggcagg tgcagccgat ggtggtcagc ccggacaaac gttatctcta tgttggtgtt
    27181 cgccctgagt ttcgcgtcct ggcgtatcgt attgccccgg acgatggcgc actgaccttt
    27241 gccgcagagt ctgcgctgcc gggtagtccg acgcatattt ccaccgatca ccaggggcag
    27301 tttgtctttg taggttctta caatgcgggt aacgtgagcg taacgcgtct ggaagatggc
    27361 ctgccagtgg gcgtcgtcga tgtggtcgag gggctggacg gttgccattc cgccaatatc
    27421 tcaccggaca accgtacgct gtgggttccg gcattaaagc aggatcgcat ttgcctgttt
    27481 acggtcagcg atgatggtca tctcgtggcg caggaccctg cggaagtgac caccgttgaa
    27541 ggggccggcc cgcgtcatat ggtattccat ccaaacgaac aatatgcgta ttgcgtcaat
    27601 gagttaaaca gctcagtgga tgtctgggaa ctgaaagatc cgcacggtaa tatcgaatgt
    27661 gtccagacgc tggatatgat gccggaaaac ttctccgaca cccgttgggc ggctgatatt
    27721 catatcaccc cggatggtcg ccatttatac gcctgcgacc gtaccgccag cctgattacc
    27781 gttttcagcg tttcggaaga tggcagcgtg ttgagtaaag aaggcttcca gccaacggaa
    27841 acccagccgc gcggcttcaa tgttgatcac agcggcaagt atctgattgc cgccgggcaa
    27901 aaatctcacc acatctcggt atacgaaatt gttggcgagc agggactact gcatgaaaaa
    27961 ggccgttatg cggtcgggca gggaccaatg tgggtggtgg ttaacgcaca ctaaccgctg
    28021 atttaaccgg cgcagtctct cctgcgccgg tgtattaact tatctcctgt aacgcatgtc
    28081 tctggcgttc gacgatattg gtccacaaat tgtctttatc gtcagtccac aaattaatca
    28141 gcaaggcaaa aaagcgttca gctgccggag aaagcacggc atctttgcgg cgaataatcc
    28201 ccaatgtccg acgtataacg ggttccacca gtgggatacc aataatcgaa gaatagggcg
    28261 catggggcat tgccagacca ggaagcgccg aaatccccag tcctgcctcc actagtccta
    28321 atgacgtcga aagatgacgc acttcgtaaa accagtccag cttccagggc ttgtcggcta
    28381 gctgttgttc tatcaacaat cggttgccgc tggaggaacg tacgccaatc attttgtaac
    28441 ccactaactc ctgccattct acaagctgct ttttggccaa tggatggtca cgccgacagg
    28501 ccagcacgaa tggctcgttg accagtgggg taaaatcaat ggatgaattt gtgacgttgt
    28561 tcatgtttat gccaaagtct gattcgttac acagcaccga ttccatgcaa ttattcgtgc
    28621 cttgttccag aatccgcact ttaatattgg gatacagctc attaaattta ccgattgcca
    28681 gcggtaaaaa ataaaatact gcggtcggaa tacacgctaa cgtcaccata ccacgatgat
    28741 aagcattcat atcacgaata ttaaaaagcg tttcatcgaa tttctttatt aattcccgcg
    28801 cctccgggag caaccttttt cctgcttttg ttaacgtaac cttgcgagtt gtacgctcaa
    28861 atagttgaat atgtaaatct tcctccatct tttttattct tcgcgttaat gcaggttgcg
    28921 taatattgag taatttagcg gcattgttaa atgaactgga ctctgccagt atgacaaatg
    28981 ccttcatact tgataattca tgcttcatta ttactccgga aaatggaagc gacgattttg
    29041 ggtggctggt cgttaaaaat tttaactgca tttagccaac ttaaattaat gaaaaaatgt
    29101 tattgatcgt tgagctaaag tcattagaga tgctttgtcc ttaatgtaac catatcgcaa
    29161 taagttatgt ttttaaattg agggcattat tatgaaaaaa ataccctgcg tgatgatgcg
    29221 aggtggaacc tcgaggggcg cgttcctgtt agcggaacat ttacccgaag atcaaacgca
    29281 gtgcgataaa atattgatgg caattatggg ttccggtaac gatctggaaa ttgacggtat
    29341 tggcggcggt aatccgctga ccagtaaagt cgccattatt agccgttcca gcgatccgcg
    29401 tgctgatgtc gattatctgt ttgctcaagt tattgtccat gagcaacgtg tcgataccac
    29461 gcctaattgt ggcaatatgc tgtctggtgt tggggcattc gccattgaaa atggtttgat
    29521 tgcagcgact tcaccagtta cccgcgtacg tatccgcaac gtcaatactg gtacgttcat
    29581 cgaagctgat gtgcaaacgc caaatggtgt tgtcgagtac gagggtagcg ccagaattga
    29641 cggcgtaccg ggtactgccg caccggttgc gctcactttc ctgaatgccg ccggaaccaa
    29701 aaccggaaaa gtcttcccga ctgataatca gattgattat tttgacgatg tcccggtgac
    29761 ctgtatcgat atggcgatgc cagtcgtcat tattccggct gaatatctgg gaaaaacagg
    29821 ttatgaatta ccggcggaac tggatgccga caaagcatta ttagcacgca ttgaatctat
    29881 ccgtctacaa gcgggtaaag caatgggctt aggtgatgtc agtaatatgg ttatccctaa
    29941 acctgtgctt atttctccag cgcagaaagg cggggcaatt aatgtacgtt attttatgcc
    30001 gcattcttgc catcgcgcgc tggcgataac cggtgctatt gctatttcca gtagttgtgc
    30061 attggaaggc accgtcaccc gacaaatcgt cccttctgta ggatacggca atatcaatat
    30121 tgaacacccc agtggtgcgc tcgacgttca tttaagtaat gaaggtcagg atgccacgac
    30181 gttacgcgca tctgttattc ggacgaccag aaaaatattt tccggtgaag tttatcttcc
    30241 ctgaaaaaat tcgttgtcag gataaggaca atcaataaag gacttctgta tgagtcatac
    30301 agaaagaaca ggattttaaa tgaataagaa atcgttatgg aagctaattc tgatattagc
    30361 gatcccatgt attattggtt ttatgccagc tccggcagga ttaagcgaac tggcgtgggt
    30421 gctttttggt atttacctgg cggccattgt ggggctggtt atcaagcctt tcccggaacc
    30481 tgtcgtactg ttaattgccg ttgctgcctc catggtggtg gtcggtaact tatccgacgg
    30541 tgcgtttaag accaccgccg tattaagcgg ttactcttca ggtactacct ggctggtgtt
    30601 ctcggcgttt accttaagcg ccgcatttgt gaccaccggt ttaggtaaac gtattgccta
    30661 tctgctgatt ggtaaaatcg gtaacaccac gctgggtctg ggttacgtta cggtattcct
    30721 cgatctggta ctggctccgg caacaccgtc taacaccgcg cgtgcgggcg gtattgtgtt
    30781 accgatcatc aacagcgtgg cggtggcttt ggggtccgaa ccggaaaaaa gtccgcgtcg
    30841 tgtcggacat tacctgatga tgtccattta catggtcacc aaaaccacca gctatatgtt
    30901 ctttaccgca atggcgggga acattctggc gctgaaaatg atcaacgaca ttctgcacct
    30961 gcaaattagc tggggtggat gggcactggc cgccggattg ccgggcatca ttatgctgat
    31021 ggtcaccccg ctggtgattt ataccatgta tccgccagaa attaagaagg tggataataa
    31081 aaccatcgct aaagcgggcc ttgccgaact gggaccgatg aaaatccgcg aaaaaatgct
    31141 gctcggtgtc tttgtgctgg cgctgctggg ctggattttc agtaagtctc tgggggttga
    31201 tgaatccact gtggcaatcg ttgttatggc gaccatgctg ctgctgggta tcgttacctg
    31261 ggaagacgtg gttaaaaata aaggcggctg gaatacctta atctggtacg gcggtattat
    31321 cggattaagc tccttattat cgaaagttaa attcttcgaa tggttagctg aagtctttaa
    31381 aaataacctg gcatttgatg gtcacggtaa cgttgctttc ttcgttatta ttttcctcag
    31441 cattatcgtg cgttatttct tcgcttccgg tagtgcctat atcgttgcta tgttaccggt
    31501 atttgccatg ctggcgaacg tctccggcgc accgttaatg ttaaccgcgc tggcactgtt
    31561 gttctccaac tcctatggcg gcatggttac tcactatggc ggcgcggcag gtccggtcat
    31621 ctttggcgtg ggttataacg atattaaatc ctggtggttg gtcggtgcgg tactgacgat
    31681 attaaccttc ctggtgtata tcaccctcgg cgtgtggtgg tggaatatgc tgatcggctg
    31741 gaacatgctg taaatattct cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct
    31801 caccccagtc acttacttat gtaagctcct ggggattcac tcgcttgtcg ccttcctgca
    31861 actcgaatta tttagagtat atccatttat tatctttctg cgcacttcac ggtgcgcaga
    31921 tatctggagc atttgatgat caagttatct gaaaaaggcg tgtttctcgc cagtaataac
    31981 gaaataattg ccgaagaaca tttcaccggc gaaattaaaa aagaagaagc caaaaaaggc
    32041 actattgcct ggtctattct ctcttcgcat aatacgtccg gaaatatgga taaacttaaa
    32101 attaagtttg attcattagc ctctcacgat attacctttg ttggtattgt acagaccgct
    32161 aaagcgtccg gtatggaacg tttcccgctg ccgtatgtgc tgaccaactg ccataactca
    32221 ctctgcgccg tcggcggcac tattaacggt gatgaccatg tttttggttt atcggcggcc
    32281 cagcgttatg gcggtatttt tgtgcctccg cacattgcgg tcatccatca atatatgcgt
    32341 gagatgatgg caggcggcgg caaaatgatc ctcgggtcag acagccacac ccgttacggt
    32401 gcattaggga caatggcagt cggtgagggc ggcggtgagt tggtaaaaca gctgcttaat
    32461 gacacctggg atatcgacta tccgggcgtg gttgcggtgc atctgaccgg aaaacctgcg
    32521 ccgtatgtgg ggccacagga tgtggcgctg gctatcattg gcgcggtgtt caaaaacggt
    32581 tacgtcaaaa acaaagtcat ggagttcgtt ggaccgggcg ttagcgcgct ctctaccgat
    32641 ttccgtaaca gcgttgacgt gatgaccacg gaaaccacct gtttaagctc cgtctggcaa
    32701 actgatgaag aagtgcataa ctggctggcg ctgcacggtc gcggccagga ttactgccag
    32761 cttaaccctc aaccgatggc gtactacgat ggctgcatca gcgtcgattt aagcgccatc
    32821 aaaccaatga ttgcgctgcc gttccacccg agcaacgtgt atgaaatcga cacactgaac
    32881 cagaacctga ccgacattct gcgtgagatt gaaattgagt ccgaacgcgt ggcgcacggt
    32941 aaagccaaac tctcgctgct ggataaagtg gaaaatggtc gcctgaaagt gcagcagggg
    33001 attatcgcgg gctgttctgg cggtaactac gaaaacgtca tcgcggcggc gaatgcactg
    33061 cgcggtcaat cctgtggcaa tgacaccttc tcgctggcag tttacccgtc atcacagccg
    33121 gtgtttatgg atctcgccaa aaaaggtgtg gtagcagatt tgattggcgc aggcgatacg
    33181 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
    33241 tctaagccag ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
    33301 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
    33361 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
    33421 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
    33481 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc
    33541 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
    33601 ctggcggagt ttaccctgtc acgccgcgat cccggttatg ttggcagaag taaagcgact
    33661 gctgagctgg aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg
    33721 cgcattaagc agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc
    33781 atggtatatg cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag
    33841 cgtgtgattg gcggtctggc gaatattgcc gaagagtacg cgactaaacg ctaccgttct
    33901 aacgtcatca actgggggat gttaccgctg cagatggcgg aagtgccaac ctttgaagtg
    33961 ggggattaca tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt
    34021 aaaggttatg tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt
    34081 ctgactgctg aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac
    34141 cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcat
//
LOCUS       DAZFAH010000028        34123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFAH010000028 DAZFAH010000000
VERSION     DAZFAH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.028"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..736
                     /locus_tag="ACHJWB_001284"
     CDS             <1..736
                     /locus_tag="ACHJWB_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4312943.1"
                     /translation="FNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVR
                     LENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSL
                     PKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLAL
                     KWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLN
                     GKASSAKVHFYAINDFGGAIEGNASL"
     gene            771..3371
                     /gene="htrE"
                     /locus_tag="ACHJWB_001285"
     CDS             771..3371
                     /gene="htrE"
                     /locus_tag="ACHJWB_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO4312944.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3388..3957
                     /locus_tag="ACHJWB_001286"
     CDS             3388..3957
                     /locus_tag="ACHJWB_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4312945.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRITKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3972..4574
                     /gene="yadL"
                     /locus_tag="ACHJWB_001287"
     CDS             3972..4574
                     /gene="yadL"
                     /locus_tag="ACHJWB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO4312946.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4601..5197
                     /locus_tag="ACHJWB_001288"
     CDS             4601..5197
                     /locus_tag="ACHJWB_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4312947.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAASRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDILSGQVMSHILVDVYYE"
     gene            5247..6503
                     /locus_tag="ACHJWB_001289"
     CDS             5247..6503
                     /locus_tag="ACHJWB_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO4312948.1"
                     /translation="MMKTFFKYFLFLVLSCSCFTAIGNPTSFLVGNNAGVGDGNFKGP
                     STAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDG
                     LMDIENAMVDSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYVGDTT
                     PQKFQWIITDPNLQHDGCDNAIWYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQQVSLS
                     SSSTYLYAFKAYSPGTRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKM
                     GEYAPGQIKNGATPLPFDISLENCVRVGDIETKLSSGKLGTENKQLLGNTITGSDAAK
                     GVGVLIEGLANSKSALMILKPNDSASVYKDNTGQTQNNDSDAIYPEDQGITYPLHFQA
                     TLKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6616..7410
                     /gene="panB"
                     /locus_tag="ACHJWB_001290"
     CDS             6616..7410
                     /gene="panB"
                     /locus_tag="ACHJWB_001290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4312949.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVEQAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7422..8273
                     /gene="panC"
                     /locus_tag="ACHJWB_001291"
     CDS             7422..8273
                     /gene="panC"
                     /locus_tag="ACHJWB_001291"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4312950.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(8551..9453)
                     /gene="rpnC"
                     /locus_tag="ACHJWB_001292"
     CDS             complement(8551..9453)
                     /gene="rpnC"
                     /locus_tag="ACHJWB_001292"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4312951.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGMSMMTLAQWFEEKGIEKGIQQGRQEVSQEFAQRLLSKG
                     MSREDVAEMTNLSLAEIDKLINSN"
     gene            9727..10107
                     /gene="panD"
                     /locus_tag="ACHJWB_001293"
     CDS             9727..10107
                     /gene="panD"
                     /locus_tag="ACHJWB_001293"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4312952.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIANFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10111..11340)
                     /locus_tag="ACHJWB_001294"
     CDS             complement(10111..11340)
                     /locus_tag="ACHJWB_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4312953.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDCQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11404..11844)
                     /locus_tag="ACHJWB_001295"
     CDS             complement(11404..11844)
                     /locus_tag="ACHJWB_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4312954.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGELLQCWAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11949..12719)
                     /locus_tag="ACHJWB_001296"
     CDS             complement(11949..12719)
                     /locus_tag="ACHJWB_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4312955.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12716..13642)
                     /locus_tag="ACHJWB_001297"
     CDS             complement(12716..13642)
                     /locus_tag="ACHJWB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4312956.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFSQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13751..14413
                     /gene="can"
                     /locus_tag="ACHJWB_001298"
     CDS             13751..14413
                     /gene="can"
                     /locus_tag="ACHJWB_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4312957.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14454..14990)
                     /gene="hpt"
                     /locus_tag="ACHJWB_001299"
     CDS             complement(14454..14990)
                     /gene="hpt"
                     /locus_tag="ACHJWB_001299"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4312958.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15196..17586
                     /gene="gcd"
                     /locus_tag="ACHJWB_001300"
     CDS             15196..17586
                     /gene="gcd"
                     /locus_tag="ACHJWB_001300"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4312959.1"
                     /translation="MAINNTGSRRLLITLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVGEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17633..19183)
                     /gene="cueO"
                     /locus_tag="ACHJWB_001301"
     CDS             complement(17633..19183)
                     /gene="cueO"
                     /locus_tag="ACHJWB_001301"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4312960.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWGISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19349..19696
                     /locus_tag="ACHJWB_001302"
     CDS             19349..19696
                     /locus_tag="ACHJWB_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4312961.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19802..20668
                     /gene="speE"
                     /locus_tag="ACHJWB_001303"
     CDS             19802..20668
                     /gene="speE"
                     /locus_tag="ACHJWB_001303"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4312962.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTYVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20684..21478
                     /gene="speD"
                     /locus_tag="ACHJWB_001304"
     CDS             20684..21478
                     /gene="speD"
                     /locus_tag="ACHJWB_001304"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4312963.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21515..21877)
                     /gene="yacL"
                     /locus_tag="ACHJWB_001305"
     CDS             complement(21515..21877)
                     /gene="yacL"
                     /locus_tag="ACHJWB_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4312964.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22052..24649)
                     /gene="acnB"
                     /locus_tag="ACHJWB_001306"
     CDS             complement(22052..24649)
                     /gene="acnB"
                     /locus_tag="ACHJWB_001306"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4312965.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLVNPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25004..26761
                     /locus_tag="ACHJWB_001307"
     CDS             25004..26761
                     /locus_tag="ACHJWB_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4312966.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGCHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRHPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(26832..28256)
                     /gene="lpdA"
                     /locus_tag="ACHJWB_001308"
     CDS             complement(26832..28256)
                     /gene="lpdA"
                     /locus_tag="ACHJWB_001308"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4312967.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28464..30356)
                     /gene="aceF"
                     /locus_tag="ACHJWB_001309"
     CDS             complement(28464..30356)
                     /gene="aceF"
                     /locus_tag="ACHJWB_001309"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4312968.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30371..33034)
                     /gene="aceE"
                     /locus_tag="ACHJWB_001310"
     CDS             complement(30371..33034)
                     /gene="aceE"
                     /locus_tag="ACHJWB_001310"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4312969.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33195..33959)
                     /gene="pdhR"
                     /locus_tag="ACHJWB_001311"
     CDS             complement(33195..33959)
                     /gene="pdhR"
                     /locus_tag="ACHJWB_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4312970.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8538 a         8911 c         8221 g         8453 t
ORIGIN      
        1 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
       61 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
      121 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
      181 tactggcgat gacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
      241 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
      301 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
      361 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
      421 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
      481 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
      541 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
      601 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
      661 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
      721 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
      781 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
      841 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
      901 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
      961 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
     1021 ttgaaattga aggaaaaaaa aacgcacaag cctgtatcac acttaagaat ttattgcagt
     1081 ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc agggatgaaa
     1141 cactcggcaa ttgccttaat ttgacggaaa ttatccctca ggcttctgtt cgttatgacg
     1201 ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa aattaccaaa
     1261 actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg tcatacaacc
     1321 tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat gctgctttta
     1381 acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac aactggatga
     1441 ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat atcgcctcgc
     1501 tgcgttctca actcattctt ggtgagtctt atacaacggg cgaaaccttt gattctgtca
     1561 gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca ttagcgagtt
     1621 ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcactatt acgcaaggtg
     1681 gctataagat ttatgaaacg actgtgccgc caggcgcttt cgtcattgat gatctgagtc
     1741 cgtcaggtta cggcagcgat cttattgtta ctgtcgaaga atccgatggc tcaaagcgga
     1801 cattctcgca acctttttca tcggtcgttc aaatgttacg ccctggcgtt ggacgttggg
     1861 atattagcgg cggccaggtc ttaaaagatg atattcagga tgagcctaat ttatttcagg
     1921 ccagctacta ctacggtctg aataactatc taacgggtta tactggtatt cagattaccg
     1981 ataataacta taccgctggt ttgttaggtc ttggtctgaa tacttcagtc ggtgcatttt
     2041 ctttcgatgt gactcactcc aatgttcgta taccggatga taaaacatat cagggacaaa
     2101 gttatcgtgt ttcctggaac aagttattcg aagaaacaag tacttcactg aatatcgcgg
     2161 cctatcgcta ttcgacacag aattaccttg gtcttaatga tgcactaact ctaattgatg
     2221 aagtgaaaca tcccgaacaa gatcttgaac cgaaatccat gcgtaattac tcgcgcatga
     2281 aaaaccaggt tacggtcagt attaaccaac cgttgaaatt tgagaaaaaa gattacggtt
     2341 cattttatct ttccggaagt tggtccgatt actgggcttc agggcaaaat cgtagcaatt
     2401 attctattgg ctacagtaac agtgcatcct ggggcagcta cagtgtcagt gcacagcgtt
     2461 catggaatga agacggcgac actgacgata gcgtttatct cagtttcacc attcctattg
     2521 aaaaattact tggcactgaa caacggactt caggtttcca gagtattgat actcaaatga
     2581 gcagtgactt taagggtaat aaccaactca acgtcagtag cagtggctat agtgataacg
     2641 ctcgcgtcag ttatagcgta aatactggct atacgatgaa taaagccagc aaagatttga
     2701 gttatgttgg gggttatgcc agctatgaat caccgtgggg aagcctggca ggttcagttt
     2761 ctgcaaatag cgataacagt cgtcaagtgt ctctcagcac cgacggtggt tttgtattgc
     2821 atagcggcgg cctgaccttc agtaatgaca gttttagcga ctccgataca ctggcggtag
     2881 ttcaggctcc aggtgctcaa ggagcgcgaa taaattatgg caacagtact atcgatcgat
     2941 ggggttatgg tgtcaccagc gctctttctc cttatcatga aaaccgtatc gcgctggata
     3001 tcaacgatct tgagaacgat gttgaattaa aaagtaccag tgcagtagct gtaccgcgtc
     3061 agggttcagt cgtctttgct gattttgaaa ccgtgcaagg gcaatcagcc attatgaaca
     3121 tcacacgaag tgatggtaaa aatattccat ttgctgcaga tatttatgat gagcaaggca
     3181 atgtcattgg taatgttgga cagggtggac aagcatttgt tcgtggcatt gagcagcagg
     3241 gaaatatcag cattaaatgg ctcgaagaaa gtaaacccgt aagttgtctt gcgcattatc
     3301 aacaaagttc agaagcagaa aaaatagcgc aatctattat tctgaatgga attcggtgtc
     3361 agattcagta actacaaggg acgtcaaatg ataaaaataa cgccacataa aatagcgata
     3421 ctgatgggaa tattattatc accatcagta tttgcaacgg atattaatgt agagtttaca
     3481 gccactgtca aagcgacaac ctgtaacatc acacttactg gtaataacgt cacgaatgat
     3541 ggcaataata actacacatt gagaatcact aagatgggtc tggataagat cgcgaataaa
     3601 acgacagaat ctcaggctga ttttaaactg gttgctagtg ggtgcagcag tggcatcagt
     3661 tggattgata ccactctgac cggaaatgca tcatcaagct cacctaagct tattataccg
     3721 cagtctggtg attcatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact
     3781 actgatgatg ccactttcct taaacctaac agtgcggaaa agatacgctg gagcacagac
     3841 gagatgcagc ccgataaggg tcttgaaatg accgttgcgc tacgtgaaac agatgcaggg
     3901 caaggcgtac cggggaattt tcgtgcactg gcaacgttta atttcatcta tcaataagaa
     3961 ggataattaa gatgacattt aaaaatttac gttatggatt atccagcagt gtcgttttgg
     4021 ctgcctcatt gttcagcgta ccctcttatg cggcaacaga cagcattggg ctgaccgtga
     4081 ttactaccgt agaaataggt acttgtaccg ctacattagt aaatgactct gatcaggaca
     4141 tttctgttgt tgactttggc gatgtatata tttctgaaat caatgccaag accaaagtta
     4201 aaacattcaa actcaaattc aaagactgtg cgggtatccc caataaaaaa gcacaaataa
     4261 aattaaccaa gcgagccaca tgcgaaggta ctgctaatga cggtgcgggg ttcgcaaatg
     4321 gatccacagc cgcagataaa gcaagtgccg tcgccgttga agtctggagc accgaaactc
     4381 cggcaacagg tagcgcaaca caatttagtt gtgtgacacc agcatctcaa gaggtcacta
     4441 tctccaatgc agccaacgcg gttgtttatt atccgatgag tgctcgactg gtcgtcgaaa
     4501 aaaataaaac cgtaagcaat gtcactgcgg gtaaattttc tgcacctgcc acatttacag
     4561 taacctataa ctaacagact gacagatcca ggaactatat atgcatccca ctcaacgtaa
     4621 gctgatgaag agaataattc tgtttctgtc actacagttt cccattacct atccagccat
     4681 cgctgggcag gatattgacc ttgttgccaa tgtaaaaaac agcacttgca aaagcggaat
     4741 cagtaaccag ggtaatattg atcttggcgt cgttggggtg ggatattttt cagataatgt
     4801 tactcctgaa agctatcaac caggtggaaa agagttcact gtcacagtat cagactgtgc
     4861 attacaggga actggcgatg tgcttaatca gttacatatt gattttagag cccttagcgg
     4921 tgtcatggct gcagcttcaa ggcaaatatt tgccaatgaa gtcgcaaccg gggctaaaaa
     4981 tgtcggtgtc gttctctttt ccattcagga cccgaccaat atattcaatg ttattagttc
     5041 agcaggaaat tctcgttccg tgtatccagt aatgacttct gccctgcata attcttcatg
     5101 gaaattttat gcccgaatgc aaaaaatcga tcccgcacta gatatcctat ctggtcaggt
     5161 aatgagtcat atattagttg atgtgtacta cgaataatag atcaaaacaa acgactgcta
     5221 ttatgacagc agcgaatggt ggggtaatga tgaagacatt cttcaaatac tttctttttt
     5281 tggtgctatc ctgttcttgc tttacagcga ttggcaatcc tacaagtttt cttgttggta
     5341 acaatgccgg ggtaggcgat ggtaatttca aaggaccgag cactgcggct caggtcactt
     5401 ttagatatgc atcaacaacc aataatttag ttttttataa acctacgcaa cttggcccga
     5461 caggtgttaa attgcgctgg gaacaattag atacagccag tggtggcggt tttctttact
     5521 gtaatgcctc tgacagggca aatccagatg gactaatgga tattgaaaat gccatggttg
     5581 attctggtaa aacctatggc gggcataagt tatttaaaac ctctgtccct ggcttgtatt
     5641 acaccctatt tattgcaaaa ctttggtctg catattcaac aaaaacgact attagcgact
     5701 ctggtttata tgttggagat accacacccc aaaaattcca gtggatcatc acagacccta
     5761 atctacaaca tgacggctgc gataacgcga tatggtacga tagattctgg gccatcggcg
     5821 gtgttgtaca cgaccttacg attgaatttt atactgatac cgattttaac cctacaacaa
     5881 accagcaagt atccttatcc agctcatcga catatcttta tgcgtttaag gcctatagtc
     5941 ccggtactcg tgttgttgat cacagtcatc acctttatat cgactttgat ctactcaatg
     6001 ttaaattaac caaccccacc tgctttactg ctgtacttac cggaaaatca gtaagtggtt
     6061 cgacggtaaa aatgggagaa tatgcaccag ggcagataaa aaatggcgcg actccgcttc
     6121 cttttgatat ttctctcgaa aactgtgttc gcgttggtga tatagagaca aagctatctt
     6181 cagggaagct gggtacagaa aataagcaac tccttggtaa cacaatcact ggaagcgacg
     6241 cagcaaaagg cgttggggta ttgattgaag gattagcaaa cagtaaaagt gccctaatga
     6301 tactgaagcc gaatgattca gcttctgttt ataaagataa tacgggccag acgcagaata
     6361 acgattcaga tgcaatttac ccagaagatc aaggtataac gtatccactt catttccagg
     6421 cgacgttaaa gcaagacggg aatattgcta tcgaacccgg cgaatttaaa gccaccagta
     6481 ctttccaggt aacctatccc tgataacatt gtggcaccgt ctggtaatgg cggtgtcaca
     6541 acagagaaat cattgccccc accaatgtga ctgataaaat gccagacagc acaacatcat
     6601 ttatcaggat accttatgaa accgaccacc atctccttac tgcagaagta caaacaggaa
     6661 aaaaaacgtt tcgcaaccat caccgcttac gactacagct tcgcgaaact gtttgctgat
     6721 gaaggactta acgtcatgct ggtaggcgat tcgctgggca tgacggttca gggccacgac
     6781 tctacccttc ccgttaccgt cgaagatatc gcctatcata ccgccgccgt acgtcgcggc
     6841 gcgccaaact gcctgctgct ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
     6901 gccttcgaaa acgccgcaac ggttatgcgt gccggtgcta acatggtcaa aattgaaggc
     6961 ggtgagtggc tggtagaaac cgtacaaatg ctgaccgaac gcgcggttcc tgtatgtggt
     7021 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt tcaggggcgc
     7081 ggcgatgaag cgggcgatca actgctcagc gatgcattag ccttagaagc tgctggggca
     7141 cagctgctgg tgctggaatg cgtgccggtt gaacaggcaa aacgtattac cgaagcactg
     7201 gcgatcccgg ttattggcat tggcgcaggc aacgtcactg acgggcagat cctggtgatg
     7261 cacgacgcct ttggtattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     7321 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     7381 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     7441 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgt gtggcgctgg
     7501 tgcctaccat gggtaacctg cacgatggcc atatgaagct ggtcgacgaa gccaaagccc
     7561 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     7621 atctggcacg ttatccacgg accttgcagg aggactgcga aaagctaaac aaacgtaaag
     7681 tagatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     7741 cttacgttga cgttcctggt ctttccacca tgctggaagg tgccagccgt cctggccatt
     7801 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagccg gacatcgcct
     7861 gcttcggtga aaaagatttt cagcaactgg cgctgatccg caaaatggtt gccgatatgg
     7921 gcttcgatat tgagatagtc ggtgtgccaa ttatgcgcgc caaagatggt ctggcgctga
     7981 gttcccgtaa cggttatctg acggcggaac aacgtaaaat tgcgcccggt ctgtacaaag
     8041 ttttaagttc gattgccgac aaattgcagg ctggcgaacg ggatctcgat gaaattattg
     8101 ctattgccgg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     8161 atgccgacac gctgctggaa gtctctgaaa ccagcaaacg agctgtgatt ctggtagccg
     8221 cctggcttgg cgatgcccgc ctgatcgaca ataaaatcgt cgaactggcg taatgctttt
     8281 actctaaata attcaagttg caggaaggcg gcaagcgagt gacaaattcg tcaggaacga
     8341 atttgcccag ccgcaggctg gccttcggtg agagacatgg atgtctctca ttaatcccca
     8401 ggagcttaca taagtaagtg actggggtga gcgaacgcag gcgcagcaca tgcaacttga
     8461 agtatgacga gtataactgg cgctacggct gatggcgcca attattaatt gccacgttat
     8521 ctgtaagcaa ttattcataa cgcagacaac ttagttagag ttaattagct tatcaatctc
     8581 agcaagagat aaatttgtca tctctgcaac gtcttcccga gacatccctt tactcagaag
     8641 acgctgggcg aattcctgac ttacttcctg tcttccctgc tgaatcccct tctcaatccc
     8701 tttctcttca aaccactgcg ccagcgtcat catagacatc cctcccgttt ccctgtctct
     8761 caccacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg
     8821 catggcaact aactgacttc cgctagtgta cccttcgtcg atcagcgtga ccagttgctc
     8881 cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat
     8941 ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg
     9001 actgttatag acgcgtcgcg ccagctccgg cgagtaaaac atatcaaacc agcacattga
     9061 tagcggataa ggtgtggcct cgccctgata aaacagtatc ggcaccacca gcggcagctt
     9121 atcgtgatcc gcctccagat gccggtgcat ggcagcaata gaataacgca tcatgcgaaa
     9181 ggccattttc ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt
     9241 gccctgcatt tgtacggaat agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat
     9301 gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttcgcgca attccactgg
     9361 caaatgtatc tccagaaagt cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac
     9421 cgcgtcatgc ggtgtggtac tcggtgcatc catcttccct gtcttccgtg ttgtcttgcg
     9481 tgaaggcagt atccagtcaa ggcgtgctat cgtcagccac ctctttattg tttaccgagc
     9541 cgcgttcaga gaaatatcaa ttcgttacag gcgatacata gcacgcttcg gcgcggtacg
     9601 caaactgctg atgcaaatgc cgtcagatga gtagacacta aacaaaaatc gggcaatact
     9661 gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt aacgacaggg tagaaaggta
     9721 gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg
     9781 gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt
     9841 ctcgaaaacg aagccattga tatctggaat gtcaccaacg gcaagcgttt ctccacttat
     9901 gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc
     9961 gccagcgtcg gcgatattgt catcatcgcc aacttcgtta ccatgcctga tgaagaagct
    10021 cgcacctggc gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg
    10081 aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac tcaccagccg
    10141 cgacatcgtc tcgagcgaat ccgttcttaa aatataaagt cgttttagta acaacggatt
    10201 atcccccggt tttactttgc ctttcatagt tgtcaccgcc aggtgaaatc cggcatcgtt
    10261 tgctgccttc acggctttgt cattaaagcc gccaaacgga tacgaaagat accagacatg
    10321 tggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc
    10381 actacggctc agtaatatgg gtcggcgata accatctacc cgatgcaaaa aatgggtatg
    10441 tgactggcaa tcaaaaacat cgcgaatttc gttaagctca gaaacgctca taaattgcag
    10501 cgattttggg tcccatttct gcgggtgacg tttgatgcgt gaggtaacaa taaacgccgt
    10561 cgccttcatg ccatattgtt tcaacacagg atacgcatag cggctcaccg acttgaggcc
    10621 atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc
    10681 ttccagctgc gccatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatctggtt
    10741 attgaaagcg cgtactgagg tggtcgtcga agtatgacga aaacgggtgt tttcttcgtc
    10801 gcgcagaata tggtgatagg ttagcaccgg caggccatta tcgggttggg catccagcgc
    10861 gctgatatag gccagtcgat cgccaatacg aacctgatac caggtttgat ttaacctgtc
    10921 tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact
    10981 gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaactaagt tctgattact
    11041 cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc
    11101 gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatacgt aataacttgc
    11161 ggcagtgggc tccacggcaa tgatttgtcc ggcccgaata ttccccacgg tcaccatctt
    11221 gtcaccaatt tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa
    11281 cgcggcactg acacttgcgg taaacagcat cagcaggaga ataacagctt gtttatacat
    11341 gaaaaaataa ccgagggtga aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc
    11401 gggttaatac tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgttggtg
    11461 ccaaagacta ctcacctccg gcccacccag ttcgacaata cgctcacgaa actctgaact
    11521 ggtcatggtt tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat
    11581 cacttcgcaa cgggagtgtt tatggcttaa taatgaagcc acgcggtacg gtggcgctcc
    11641 ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca
    11701 catcatgcgg ctgagcatat tagagcttaa tccgcgccag aaattcacgg cccagcactg
    11761 aagaagttcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg
    11821 acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga
    11881 ccattgttga taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg
    11941 aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa
    12001 aacgccacaa taaagaccac cagtacgcca aaggtagtga ccagcggcac atcattgata
    12061 ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc
    12121 ccttgccaga acggcggcaa caaagtcagg gagtaaaaca ccccgcccaa atacgtgagt
    12181 ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacgccg
    12241 ttcagcaaac ccgcaaggga gaatagcacc gccgtgagca ccagcgttaa ggcaacgaat
    12301 acccacgaat gcacctgaaa tggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca
    12361 acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga
    12421 accggcgcta ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac
    12481 gcgacgttgg cgtaggcatt ggtgatcacc gacatcatga tcagccccgg tacgatgaac
    12541 tgcatgtagc tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaaaata
    12601 ataaagtaaa gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata
    12661 aagcgatgga tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg
    12721 cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt
    12781 tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgaaaat acgctgttga
    12841 tcccctgctc acgcagcact tcaacttcca gcgtcgcagt atcgaccagt cgatactgat
    12901 agccatcgag cttcggtaac gggcttttcg gtgcgagatc gagaataaag gtttccgatt
    12961 tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa
    13021 tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg
    13081 tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat
    13141 ccacgcctgc agtcggctcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg
    13201 caatcattaa acggcgcttc atcccgccag ataacatgcg cgcacgttcg ttgcgttttc
    13261 cccataaatc gagctgctta agatattttt cgctgcggat gtacgcctct ttgcgctcca
    13321 caccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt
    13381 taaattcctg cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat
    13441 cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga
    13501 tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga
    13561 cctgcaaatc tattccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt
    13621 gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt
    13681 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc
    13741 gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat
    13801 gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt
    13861 tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc
    13921 gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacactg acctgaactg
    13981 cctttccgtg gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg
    14041 ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa
    14101 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat
    14161 gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa
    14221 cctgggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca
    14281 cggctgggcc tatggcattc acgacggctt gctgcgtgat ctggatgtta ctgccaccaa
    14341 ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc
    14401 caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg
    14461 tccagcagaa tcactttgcc gatatacggc agatgacgat aacgctgggc gtaatcaatg
    14521 ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc
    14581 acttcacgac gggacggttt atccagcagc gtacaaatcg ccagcgactt cggttcgcgc
    14641 aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca
    14701 atcagcacgt ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg
    14761 gtggtggaca tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc
    14821 tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag acccaccagc
    14881 accatatcgc tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata
    14941 cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc
    15001 atatgattta aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa
    15061 gacacgcagt ataccagtaa aactattcat tcgcggaatc tgttaataaa gctcgaattg
    15121 tgatgacgat cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg
    15181 aaatggtgtc tctttatggc aattaacaat acaggctcgc gacgattact catcacgcta
    15241 acagcccttt ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc
    15301 gcgattggcg gctcctggta ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg
    15361 atgctgtggc gcagtaaacg cgccgcgctt tggctgtacg cagccctgct gctcggcacc
    15421 atgatttggg gcgtcgggga agttggtttc gacttctggg cgctgactcc gcgcagcgac
    15481 attctggtct tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt
    15541 cctgccagcg gcgcagttgc cgcactggtg gtcgcactgc tgattagcgg tggtatcctg
    15601 acctgggccg gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca
    15661 cctgccgaag ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag
    15721 gaaggtcaac gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa
    15781 gaagcctggg tgttccgtac tggcgatgtg aagcagccta acgatccggg tgaaatcacc
    15841 aatgaagtga cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc
    15901 ctgtttgcac tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa
    15961 accaacgagt ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca
    16021 gaaacggctt cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat
    16081 gatggtcgcc tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat
    16141 aaaggcgtgc tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgagccg
    16201 acttcgccgc cgattatcac cgataaaacc atcgtgatgg ccggttcagt taccgataac
    16261 ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg
    16321 ctgtgggctt ttgatcccgg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc
    16381 tttactttta actcgccaaa ctcgtgggca ccagcggcct atgacgcgaa gctggatctg
    16441 gtctatctgc cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa
    16501 caggaacgtt atgccagctc gattctggcg ctgaatgcca caaccgggaa attggcgtgg
    16561 agctaccaga ccgttcacca cgacctgtgg gatatggatc ttccggcaca gccgacgctg
    16621 gcggacatca ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc
    16681 aacatttttg tgctcgatcg tcgtaatggc gaactggtgg ttccggcacc ggaaaaaccg
    16741 gttccccaag gtgctgccaa aggcgattac gttaccccta ctcaaccatt ctcggaactg
    16801 agcttccgtc cgacgaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac
    16861 caactggtgt gccgcgtgat gttccaccag atgcgctatg aaggcatttt caccccgcca
    16921 tctgaacagg gtacgctggt cttcccgggt aacctgggaa tgttcgaatg gggcgggatt
    16981 tccgttgatc caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg
    17041 aaactgatcc cacgcggtcc gggtaacccg atggaacagc cgaaagatgc caaaggcacg
    17101 ggtacggaat ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg
    17161 ttcctctcac catttggtct gccatgtaaa cagccagcat ggggttatat ctcggcgctg
    17221 gatctgaaaa ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca ggacagtatg
    17281 ccgttcccga tgccggttcc ggtgccgttc aatatgggta tgccgatgct gggcgggcca
    17341 atctccacgg cgggtaacgt gctgtttatc gccgctacgg cagataacta cctgcgcgct
    17401 tacaacatga gcaacggtga aaaactgtgg cagggtcgtt taccagcggg tggtcaggcg
    17461 acgccaatga cctatgaagt gaatggtaag cagtatgtgg tgatctccgc aggcggtcac
    17521 ggttcatttg gtacgaagat gggcgactat attgtggctt atgccctgcc ggatgatgtg
    17581 aaataaccat aatgcccgga gagatccggg catatttccg aatacggtct ttttataccg
    17641 taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag
    17701 catgttcttt cggtgcgtcg tgattaaact tcaccagcac ttcactgaca ttgccttcta
    17761 ccttaacggt atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca
    17821 agatacggaa ctgcgtgccg tgaatatgga acggatgcag catcatgtcg cccacgccag
    17881 agatacccca acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa
    17941 acgcctgacc gttgatttta ttggcatggt ggaaatcgaa cttcccgccg tggttcatat
    18001 gattcatatt gccgtgcccc atatggccca tcatctggct atgatccatc ccggccatcg
    18061 cctgatcgcc atatttctcc attagcatct gcatccccat catatcgagc atcgggtcca
    18121 tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac
    18181 ttaatgtgtc tggcaaagca ccggaggcac taatagcaat cggctgaatc cgcattaccg
    18241 gatgaggctt atcaaacggc gcaatcgcca tccccatctg gctgaccggc agcgtcacca
    18301 ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca
    18361 ccggcagctc gttcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat
    18421 aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattgagca
    18481 aacgcaggcg cagccaacca cgcggggcag cgtgttgcgg gtagattgca ccgttggtca
    18541 gcaacgtatc gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct
    18601 gcccgtcggc gctaaatttc ttatcctgaa cgatcaccgg aacatcatcg ataccccact
    18661 gttttggcag cattaatttc aggatctcgt catcttcaat caccaccaga ccagccagcc
    18721 ccatcgccac ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caggtagcgg
    18781 caggttgatc aacgttcaac gtcaccgagc gcttgccacc tggcggaata attccctgtg
    18841 ggccaccgtc gacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg
    18901 tcagttggtt atagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc
    18961 ccagcagatt gccgttatag ccccaggtag ttgcagtttt ctcaccaaag gtagactgac
    19021 ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg
    19081 gtaacgttgg gcgttccgcc gcaaagactg cacggctcca cagtggcaag gctgaagcca
    19141 cacccagcgc gacggaatat ttcaagaaat cacgacgttg catagttatt tccttattct
    19201 taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag
    19261 ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt
    19321 tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg
    19381 cagcctgatg gccgtttgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat
    19441 ggccgattta acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc
    19501 taacgggcaa attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag
    19561 taattacgac accttcgaca tgaaagctct cggtgaagac agctaccgcg atctcagcgg
    19621 cattggcatt cctgtcgcta aaaaatgcaa agccctggcc cgcgattcct taagcctgct
    19681 tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt
    19741 caatgttagc tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc
    19801 catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc
    19861 ggtagataac gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa
    19921 cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga
    19981 gtttatctat catgagatga tgacctatgt tccgctactg gcccacggtc acgcgaaaca
    20041 tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa
    20101 cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc gtatcgttct gccgtcagta
    20161 tctacccaac cataacgccg gtagctacga cgatccgcgc tttaagttgg tgatcgatga
    20221 tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct ccgactgcac
    20281 cgatcctatc ggtcccggcg aaagcctttt cacttcggca ttttatgaag gctgcaaacg
    20341 ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga
    20401 agaagccatc gacagccatc gcaaactcag ccattacttc agcgacgttg gcttttatca
    20461 ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga
    20521 cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa
    20581 atgccgttat tacaatccag cagtccatac ggcagctttt gccttacctc agtatctgca
    20641 agacgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact
    20701 gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc
    20761 caaaactgcc gaagaacgcg acggttatat tgcttatatc gatgaactct ataatgccaa
    20821 ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat
    20881 cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc
    20941 ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt
    21001 tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg
    21061 cggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct
    21121 gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg
    21181 cgtgcgcggt tttactcgcg acattaacgg tatgaagcac tttatcgacc atgagattaa
    21241 ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtaaa
    21301 cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca
    21361 ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc
    21421 tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg
    21481 gctctggcgg agctccaggc tccgccagat ttatttactt ctgctgcacg aaattgcggt
    21541 aagccgccac gacctgcaga aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt
    21601 agtagttcat cccctcttcc atttcatcgc cagcgaattc cagttgattg gcgcgaacca
    21661 tcacctcttc accgtccatc cacagggtgt attcatgccc tgcccgttgc caggaccgtt
    21721 cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc aggttttctt
    21781 tcacctcttc attaaaccag tgcccgacca cttcatgccc catggacata cgcaccttta
    21841 ccactccggt aatatcgcgc agaaattcgt aatccatagt gtgttcctct gctcccggca
    21901 atgcgccgaa atcgcattgc gccattgatg taattatcgc agccttaccg gagaaaaaca
    21961 gcggaacggc gatggtttag aaataaaaaa tgcccgggaa tctcattcac cgggcattgt
    22021 gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc agtctggaaa atcaccccat
    22081 cggctttttc ggtgtactga gaaagctggt tgaagttcag ataacgataa gtatcaacgg
    22141 cagttttatc tacttgcgca acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
    22201 tcagcgccgc aacagccgcc agttctgcag aagccaggaa gacattcgcg ccagtcccca
    22261 gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg
    22321 cctggttacc catacacagg gaacagccag ggatctcgat acgcgcaccg ctcttaccga
    22381 agacgctata gtagccttct tcggtcaact gcgcggcatc catacgggtt ggcggtgcca
    22441 cccacaggcg cgtcggcaac tggcctttgt gcgcatccag cagtttaccc gcagcacgga
    22501 agtgaccgat gttggtcatg caggaaccga taaacacttc gtcgatcttc tcgccctgta
    22561 ccgcagacag cggacgtgcg tcgtccgggt cgttcggtgc acagaggatt ggctctttaa
    22621 tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    22681 gctcaggatt caccagccat ttttccatgc cctgaatacg acgttccagg gtacgacgat
    22741 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttagagttc aggtattcga
    22801 tgatcggttc tttgttcagc ttgatggtac aaccagcggc agaacgctcg gcggacgcat
    22861 cggtcagttc aaatgcctgc tcaactttca gatccggcag accttcaatt tccagaatgc
    22921 gaccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    22981 catacagcgg gatcgcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    23041 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa
    23101 atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    23161 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tggattacgc
    23221 cgtcacccgg acgcagcgac acaccaccac ggttcataat gaagtccggc agcgtgtggt
    23281 gcgtgttcac gtcaactggc ttcggatacg ccgcggtatg gcagaaagac tgcatcacca
    23341 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggccgg
    23401 tggtgtcctg tgaacctaca gaagtcattt tcggttcgca gtacgcgccc ggacgaatac
    23461 ctttcacacc gcaggcacga cctaccattt tctgcgccag cgagaagccg cgatcgctct
    23521 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    23581 ctttggtggt caggccacgc ccgataatca gcgggatacg gccgccagca cgcacttcat
    23641 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac
    23701 gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt
    23761 cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt
    23821 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    23881 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    23941 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    24001 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    24061 gcagtgggat atccgggcgc gaccacgcat ccggtgccgg agaaaggtca tcagtgttgg
    24121 tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    24181 ggaaccattc ggcatccgcc caggactgca ttacctgctt cgcatattcg ttgcctgctt
    24241 tggctttctc ttctacgtca tagaagttat cgaacatcag cagtgtgtga gaaagtgctt
    24301 tggcagcgat cggtgccagt ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt
    24361 aaccaccctg catggtaccc agcagttcga tggctttttc cggagtcagc agaggggatt
    24421 tggcttcgcc tttagcgata gcagccagga agcctgcttt gacataggcg gcttcatcga
    24481 cgcctggggg aacacggttg gttaatagat ctaacaggaa ttcttcttcg cccgcgggcg
    24541 ggtttttcag cagctctaca agtgcggcca tttggtttgc atccaggggt ttgggcgcaa
    24601 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    24661 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    24721 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gatgtgttaa
    24781 tctgcttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    24841 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    24901 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    24961 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    25021 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctaccgg
    25081 gctatatata aaaagccgca cagtggaagc gcctgtggaa ccgcaatcga cacaacagac
    25141 tgcgcctgac atcaccgcag ttacgcttcc tgcaacggtt tccgcaccgc ccgtaacgcc
    25201 cgccgtcgtc aaatccgcat tcagcactgc acaaatcgat caatgggttg cgcctgtcgc
    25261 gctgtacccc gattctctgc tttcacaagt gttaatggca tcaacctatc cggcaaacgt
    25321 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    25381 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    25441 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    25501 gcagccgcag gacgtgatgg actcggtaca gcgattgcgg caactggcgc aacaaaccgg
    25561 ttcgctgaag tcatcaaccg aacaaaaagt tattaccaca acgaagaaag ctgtaccggt
    25621 aaaacagaca gtcacggcac ccgtcatacc atccaatacc gttttaactg ccagccccgt
    25681 cattacagag ccggcaacaa ccgtcatttc cattgagccc gccaatcctg atgtggtcta
    25741 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    25801 ggtttatctg ccaccaccag ccggagaacc gtttattgac agctttgtgc gcggattcgg
    25861 ctatagcatg ggtgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga
    25921 cgatcatgac catcatcatc atgacgatga tgattatcat caccacgatg gctgtcatcg
    25981 tgacagtaat ggctggcaac acaacggcga caacatcaat atcgacgtca acaatttcaa
    26041 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    26101 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaaccgatgt
    26161 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc
    26221 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    26281 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    26341 ccgcgacttc agccgtcgcc accccctgac ccagcaacaa aaggacgccg ctcgtcagcg
    26401 ttatcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc
    26461 acagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca
    26521 gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa
    26581 cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggaaaaagt
    26641 tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga
    26701 gcaacggtca gcagtacgcg aacgtctctc tgagcgcgga gcaaggcgac aggaaaggta
    26761 aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg
    26821 caaacgaaca attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg
    26881 aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt
    26941 gcgatgtctt cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg
    27001 ccgccgttgg taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc
    27061 agcttggtca taccgtctgc gcagtcggaa gcgatagcac gaccagaagc agcccacggg
    27121 aaggtggcgg tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc
    27181 catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt
    27241 ttcttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc
    27301 atcggctgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt
    27361 ttgtcaacgc ggatgaaacc acggtcgtcc acttccacgc cagctttgcc tgcgtcgagg
    27421 tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca
    27481 gcgggtgctt ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg
    27541 gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta
    27601 acgatgtctt tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt
    27661 gaacccagcg cgtggtatac ggtgcccatt tccagaccga tgataccgcc acccattacc
    27721 agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca aatacgcgga
    27781 tcttcatgcg gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcattg
    27841 tcgaagttga tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg
    27901 aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca
    27961 ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc
    28021 ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct
    28081 acgtgcagca gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg
    28141 ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag
    28201 taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg
    28261 acctctatat atttatctcc ggcgggtctg acgttttttt tatgacgccc attatccacc
    28321 gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc
    28381 aaccgatttg tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc
    28441 ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg
    28501 ttaatgatgg taatgaaacg agcaccatca gcaccgtcga tcacgcggtg gtcgaaggag
    28561 agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc
    28621 gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg
    28681 gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca
    28741 gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg
    28801 atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc
    28861 gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacaacgaa
    28921 ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc
    28981 ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac
    29041 gcttccagct cggtgatatc ggttttgtcg aagtgagtaa catgcgggat catcacccag
    29101 ttacggctca ggttcgcacc agaaattttc tggatacggc ccagttccac ttcttcgatt
    29161 tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcctgggat accgccgcca
    29221 gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct
    29281 tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaac
    29341 tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt ttctgcaaat
    29401 tcagatttgc cttccgcttt cgcagccggt gctgctgccg gagcttcagc ttttgctgcc
    29461 ggagccggcg ctgccgcttc ctgtttcgcc ggagccgccg caggcgctgc gccttcaact
    29521 tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc
    29581 ttcacgacgc ctgcaaacgg agccggaact tccatagaag ctttgtcgcc ttctacggta
    29641 atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca
    29701 acttcgtcac cgccgatatc cggaacgtta acttctttca cgccagccgc tggtgcaggg
    29761 gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg
    29821 acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc
    29881 tctttcacgg tgccagcaaa cggagccgga acttccatag acgctttatc gccttctacg
    29941 gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact
    30001 tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc agccgctggt
    30061 gctgctgccg gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca
    30121 ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag
    30181 actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg
    30241 ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc
    30301 tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt
    30361 cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga
    30421 ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg
    30481 ctacaaccac gtaggaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg
    30541 aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga
    30601 cctgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca
    30661 cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc cagcgttcac
    30721 aatcctgacc atctcgcgcc agttcggtga aggaggtcac gctataaacg tcagaaccta
    30781 cgccgtaatc tttcgccagg atctctgccg cttcacggac gtgacgcagg atggaaccgg
    30841 agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt
    30901 tacggatacc ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg
    30961 tagtgatgta gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat
    31021 gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag
    31081 tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcaccg ttcagggtgg
    31141 tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc
    31201 acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaac ggaatcatcg
    31261 gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccaact
    31321 cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct
    31381 cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca
    31441 gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga
    31501 tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct
    31561 ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct
    31621 cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgtgcgtgca
    31681 gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg
    31741 catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt
    31801 tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa
    31861 tggtatgagc aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag
    31921 cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgt tcgtcagtcc
    31981 agtctgcaac cagtgctgcg gtttccggat atttaccgaa gaagtgttca cgaacgtacg
    32041 caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga
    32101 tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt
    32161 tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt
    32221 taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaag accaggttat
    32281 ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac
    32341 cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca
    32401 gatatttcag gaatttagcc tggtagatag caccaatcgg acccagaccc atagatacgg
    32461 tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc
    32521 cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag
    32581 cacgagcgta aacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct
    32641 gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact
    32701 ggaaggacgc catatggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca
    32761 tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat
    32821 actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgatacct gtgcctgcgg
    32881 ctacgtttac accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac
    32941 gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga
    33001 tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac
    33061 gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt
    33121 ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt
    33181 taccagaaaa atcactaatt ttttcgttgc tccagacgac gcagagaacg ctcacggcgg
    33241 ctctcttcac gacttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat
    33301 gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga
    33361 ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca
    33421 ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc
    33481 gcttcggtga cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca
    33541 gactgctgcg ccagctctat ggcgtgatgg agttcacgga tgcgttcctt gtcttcatcg
    33601 gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc
    33661 aagtcatact gtgactcagg atggtcggag agcagctcca ccagcggatc gctgaagctt
    33721 tgccataggc tgctctggac aaaagtaccg ccaccctggc gacgaagcaa caagcccttc
    33781 gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa ctgttttgcc
    33841 agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac
    33901 tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg
    33961 agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac
    34021 gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact
    34081 gtgcacattt taacaacttg acatatataa cgtttcaaag ttg
//
LOCUS       DAZFAH010000029        33661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFAH010000029 DAZFAH010000000
VERSION     DAZFAH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.029"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..247
                     /gene="ydhI"
                     /locus_tag="ACHJWB_001312"
     CDS             11..247
                     /gene="ydhI"
                     /locus_tag="ACHJWB_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4312971.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            250..1107
                     /gene="ydhJ"
                     /locus_tag="ACHJWB_001313"
     CDS             250..1107
                     /gene="ydhJ"
                     /locus_tag="ACHJWB_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4312972.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1107..3119
                     /gene="ydhK"
                     /locus_tag="ACHJWB_001314"
     CDS             1107..3119
                     /gene="ydhK"
                     /locus_tag="ACHJWB_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4312973.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGGIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLTRTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3120..3641)
                     /gene="sodC"
                     /locus_tag="ACHJWB_001315"
     CDS             complement(3120..3641)
                     /gene="sodC"
                     /locus_tag="ACHJWB_001315"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4312974.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3722..4618)
                     /gene="ydhF"
                     /locus_tag="ACHJWB_001316"
     CDS             complement(3722..4618)
                     /gene="ydhF"
                     /locus_tag="ACHJWB_001316"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO4312975.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4667..4906)
                     /gene="ydhL"
                     /locus_tag="ACHJWB_001317"
     CDS             complement(4667..4906)
                     /gene="ydhL"
                     /locus_tag="ACHJWB_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4312976.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5009..5608
                     /gene="nemR"
                     /locus_tag="ACHJWB_001318"
     CDS             5009..5608
                     /gene="nemR"
                     /locus_tag="ACHJWB_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4312977.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5645..6742
                     /gene="nemA"
                     /locus_tag="ACHJWB_001319"
     CDS             5645..6742
                     /gene="nemA"
                     /locus_tag="ACHJWB_001319"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4312978.1"
                     /translation="MSSEKLYSPLKVGTITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6823..7230
                     /gene="gloA"
                     /locus_tag="ACHJWB_001320"
     CDS             6823..7230
                     /gene="gloA"
                     /locus_tag="ACHJWB_001320"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4312979.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7333..7980
                     /gene="rnt"
                     /locus_tag="ACHJWB_001321"
     CDS             7333..7980
                     /gene="rnt"
                     /locus_tag="ACHJWB_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4312980.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYETLHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8073..12689
                     /gene="lhr"
                     /locus_tag="ACHJWB_001322"
                     /pseudo
     CDS             8073..12689
                     /gene="lhr"
                     /locus_tag="ACHJWB_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(12740..13087)
                     /gene="grxD"
                     /locus_tag="ACHJWB_001323"
     CDS             complement(12740..13087)
                     /gene="grxD"
                     /locus_tag="ACHJWB_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4312981.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13421..14236
                     /gene="mepH"
                     /locus_tag="ACHJWB_001324"
     CDS             13421..14236
                     /gene="mepH"
                     /locus_tag="ACHJWB_001324"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4312982.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14364..14945
                     /gene="sodB"
                     /locus_tag="ACHJWB_001325"
     CDS             14364..14945
                     /gene="sodB"
                     /locus_tag="ACHJWB_001325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4312983.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRCSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15091..16260)
                     /gene="ydhP"
                     /locus_tag="ACHJWB_001326"
     CDS             complement(15091..16260)
                     /gene="ydhP"
                     /locus_tag="ACHJWB_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4312984.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16426..16515)
                     /gene="cydH"
                     /locus_tag="ACHJWB_001327"
                     /gene_synonym="ynhF"
     CDS             complement(16426..16515)
                     /gene="cydH"
                     /locus_tag="ACHJWB_001327"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4312985.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16814..17839
                     /gene="purR"
                     /locus_tag="ACHJWB_001328"
     CDS             16814..17839
                     /gene="purR"
                     /locus_tag="ACHJWB_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4312986.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17836..18768)
                     /gene="punR"
                     /locus_tag="ACHJWB_001329"
     CDS             complement(17836..18768)
                     /gene="punR"
                     /locus_tag="ACHJWB_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4312987.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18881..20092
                     /gene="punC"
                     /locus_tag="ACHJWB_001330"
     CDS             18881..20092
                     /gene="punC"
                     /locus_tag="ACHJWB_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4312988.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPATKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20383..21531
                     /gene="cfa"
                     /locus_tag="ACHJWB_001331"
     CDS             20383..21531
                     /gene="cfa"
                     /locus_tag="ACHJWB_001331"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4312989.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21571..22212)
                     /gene="ribE"
                     /locus_tag="ACHJWB_001332"
     CDS             complement(21571..22212)
                     /gene="ribE"
                     /locus_tag="ACHJWB_001332"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4312990.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22427..23800
                     /gene="mdtK"
                     /locus_tag="ACHJWB_001333"
     CDS             22427..23800
                     /gene="mdtK"
                     /locus_tag="ACHJWB_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4312991.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVKPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(23841..25097)
                     /gene="ydhQ"
                     /locus_tag="ACHJWB_001334"
     CDS             complement(23841..25097)
                     /gene="ydhQ"
                     /locus_tag="ACHJWB_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4312992.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25405..25481
                     /locus_tag="ACHJWB_001335"
     tRNA            25405..25481
                     /locus_tag="ACHJWB_001335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25439..25441,aa:Val,seq:gac)
     gene            25486..25562
                     /locus_tag="ACHJWB_001336"
     tRNA            25486..25562
                     /locus_tag="ACHJWB_001336"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25520..25522,aa:Val,seq:gac)
     gene            25670..25975
                     /gene="ydhR"
                     /locus_tag="ACHJWB_001337"
     CDS             25670..25975
                     /gene="ydhR"
                     /locus_tag="ACHJWB_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4312993.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26101..27705
                     /gene="ydhS"
                     /locus_tag="ACHJWB_001338"
     CDS             26101..27705
                     /gene="ydhS"
                     /locus_tag="ACHJWB_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4312994.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLNTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27717..28529)
                     /gene="ydhT"
                     /locus_tag="ACHJWB_001339"
     CDS             complement(27717..28529)
                     /gene="ydhT"
                     /locus_tag="ACHJWB_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4312995.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28533..29318)
                     /gene="phsC"
                     /locus_tag="ACHJWB_001340"
     CDS             complement(28533..29318)
                     /gene="phsC"
                     /locus_tag="ACHJWB_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4312996.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29315..29983)
                     /gene="ydhX"
                     /locus_tag="ACHJWB_001341"
     CDS             complement(29315..29983)
                     /gene="ydhX"
                     /locus_tag="ACHJWB_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4312997.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30047..30685)
                     /gene="ydhW"
                     /locus_tag="ACHJWB_001342"
     CDS             complement(30047..30685)
                     /gene="ydhW"
                     /locus_tag="ACHJWB_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4312998.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVACTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30698..32800)
                     /gene="ydhV"
                     /locus_tag="ACHJWB_001343"
     CDS             complement(30698..32800)
                     /gene="ydhV"
                     /locus_tag="ACHJWB_001343"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4312999.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVQTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKRRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLISSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32821..33447)
                     /gene="ydhY"
                     /locus_tag="ACHJWB_001344"
     CDS             complement(32821..33447)
                     /gene="ydhY"
                     /locus_tag="ACHJWB_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4313000.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8072 a         8881 c         8294 g         8414 t
ORIGIN      
        1 aatctacttt gtgaagttta tgctcaatgc aacaggattg cccttacaag atctggtgtt
       61 cggtgcgtcc gtctattttc ctccgttttt caaagccttc gcgtttggat tcgtcatctg
      121 gcttgtcgta caccgcctgc ttcgtggctg gatctacgcc ggtgacatct ggcatccctt
      181 gttaatggat ttatcgctgt ttgcgatttg cgtttgcctt gctctggcaa tactgattgc
      241 gtggtaacta tgtcaattaa aacaattaag tatttctcaa caatcattgt agcgatagtt
      301 gcggttcttg ccggatggtg gctgtggaat tattacatgc aatctccgtg gacacgcgac
      361 gggaaaatac gcgcagaaaa ggtttctatc accccacagg tgtcgggacg tattgttgag
      421 ctgaatataa aagacaacca actggttaac gcgggggatc ttctgctcac gatcgataaa
      481 acgccctttc agatcgccga gctgaacgca caggctcagc tggcgaaagc gcaatctgac
      541 ctcgcaaaag ccaataacga agcgaatcgt cgtcgtcatc tctcacaaaa ttttatttct
      601 gccgaagaac tcgataccgc taacctcaat gttaaagcga tgcaggccag tgttaatgcc
      661 gcacaggcca cgctgaaaca gacacaatgg caactagcgc aaacggaaat tcgcgccccg
      721 gtgagtggtt gggtgactaa cctcactacc cgcatcggcg attacgctga caccgggaaa
      781 ccgctgtttg cccttgtcga cagccactcg ttttacgtca ttggttattt tgaagaaacc
      841 aagttgcgcc atatccgcga aggtgcacca gcacaaatta ccctctatag tgataacaaa
      901 acgttacggg gtcacgtttc cagtatcggt cgggcgattt atgatcaaag cgttgaaagt
      961 gactcaagcc ttattccgga tgtgaaacct aacgtcccct gggttcgtct cgcccaacgc
     1021 gttcccgtcc gctttgcgct ggataaagtt ccaggcgatg tcacgctggt gtcgggcacc
     1081 acctgtagca ttgccgtagg tcaataatga acgcatcgtc atggtccttg cgcaatttgc
     1141 cctggttcaa ggccacgctg gcgcaatggc gttatgcgtt acgcaatacc attgccatgt
     1201 gtctggcgct gacggttgcc tattatttga atctggatga accctattgg gcgatgacct
     1261 cggctgcagt ggttagcttt cccaccgttg gcggtgtcat cagcaaaagc ctcggacgca
     1321 tcgctggcag tttactggga gccattgcgg cactgcttct tgccgggcat acgctcaatg
     1381 agccgtggtt ttttctattg agcatgtcgg cgtggcttgg cttttgtacc tgggcctgtg
     1441 cgcacttcac gaataacgtc gcgtatgcat ttcaactggc gggctacacg gctgccatca
     1501 tcgcctttcc gatggttaat attactgagg ccagccagct gtgggatatc gctcaggcgc
     1561 gcgtttgcga ggtgatagtc ggtattttgt gcggcggaat gatgatgatg atcctgccta
     1621 gcagttccga tgctacagcc cttttaaccg cattgaaaaa catgcacgcc cgattactgg
     1681 aacatgccag tttactctgg cagcctgaaa caaacgatgc cattcgtgca gcacatgagg
     1741 gggggattgg gcagatactg accatgaatt tgctgcgtat ccaggctttc tggagccact
     1801 atcgttttcg ccagcaaaac gcgcgcctta atgcgctact ccaccagcaa ttacgtatga
     1861 ccagtgtcat ctccagcctg cgacgtatgt tgctcaactg gccctcaccg ccaggtgcca
     1921 cgcgagaaat tctcgaacaa ttgctgacgg cgctcgccag ttcgcaaaca gatgcttaca
     1981 ccgtcgcacg tattatcgcc ccgctacgcc cgaccaacgt cgccgactac cggcacgtcg
     2041 ccttctggca gcgactacgt tatttttgcc gcctttatct gcaaagtagt caggaattac
     2101 atcgtctgca aagcggtgta gatgatcatg ccagactcac acggacatcc gctctggctc
     2161 gtcataccga caacgccgaa gctatgtgga gcgggctgcg tacattttgt acgttgatga
     2221 tgattggcgc atggagtatt gcatcgcaat gggatgccgg tgccaatgca ttaacgctgg
     2281 cagcaattag ctgcgtactc tactccgccg tcgcagcacc gtttaagtcg ttgtcactgc
     2341 tgatgcgcac gctggtgtta ctttcgctat tcagctttgt ggtcaaattt ggcctgatgg
     2401 tccagattag cgatctgtgg caatttttac tgtttctctt tccactgctg gcgacaatgc
     2461 agcttcttaa attgcagatg cccaaatttg ccgcactgtg ggggcaactg attgttttta
     2521 tgggttcgtt tatcgctgtc actaatcccc cggtgtatga ttttgctgat tttcttaacg
     2581 ataatctggc aaaaatcgtt ggcgttgcgc tggcttggct agcgttcgcc attctgcgtc
     2641 caggatcgga tgctcgtaaa agccgccgcc atattcgcgc gctgcgccgg gattttgtcg
     2701 atcagctaag ccgccatcca acactgagtg aaagcgaatt tgaatcgctc acttatcatc
     2761 acgtcagtca gttgagtaac agccaggatg cgctggctcg ccgttggtta ttacgctggg
     2821 gtgtagtgct gctgaactgt tctcatgttg tctggcaatt gcgcgactgg gaatcgcgtt
     2881 ccgatccgtt atcgcgagta cgggataact gtatttcact gttgcgggga gtgatgagtg
     2941 agcgtggcgt tcagcaaaaa tcactggcgg ccacacttga agaattacag cggatttgcg
     3001 acagccttgc ccgtcatcat caacctgccg cccgtgagct ggcggcaatt gtctggcggc
     3061 tgtactgctc gctttcgcaa cttgagcaag caccaccgca aggtacgctg gcctcttaat
     3121 tacttaatta caccacaggc atagcgttca ccgccaccgc ccagcggttt aggttgatcg
     3181 gacatattat cgccgccaac gtggaccatc agcgctttgt ctttgatttc atccagtgat
     3241 ttcagacgag gcgcgatgac ggcatcggta gctttgccgt cattattgac gaccagtgca
     3301 ggcagatcgc ctaaatgccc ggcaccttct ggcccttcat gtttaccggt attttgtgga
     3361 tcaagatgcc cgcctgcgga ttccgcggcg ctggctttgc catctttggt ggctggctgg
     3421 cagcttcctt tggcatgaat atggaagcca tgttcaccgg ggggtaatgc tttcagatcg
     3481 ggcgaaaact ccagaccttt atcggtttca gtaatggtga cgctaccaat tgactgccct
     3541 accccttgcg acgtgacgag gttcatctcg actttttcac tggcagcttg tgcgccggtt
     3601 gcaacaacca gcgccagaat agccagacta aaacgtttca taggacctcc gttcattgtg
     3661 cattcctgaa taagtgtaaa ccagtgacac atttttgaac ggcggctatt cctaaaagtg
     3721 cttacggtac gtcgtacccc agtgccgctt tacggatacg aaaccattgt tgacgggtca
     3781 ttttcagtgt ttctgcttcg acagctgccc gtacgcgctc aattttacct gaaccgataa
     3841 ttggcagcgg ctgcgatggt aaacgtaata cccaggcgta aaccacctgt tcaatcgagc
     3901 ccgcgtttaa ctcctctgcc accacagcca gttcatcacg cagcggctgg aaataatcat
     3961 cattaaacag acgaccacca ccaaggcagg accacgccat cggacgaaca cgcagttgtt
     4021 gtagttggtc gagcgtgcca tccagcagta acggctgatg caccggggat atttccacct
     4081 gattagtggc aagggtaaac ggcagacgtg attgcaacag ggcaaattgc gcaggcgtaa
     4141 agttcgatac gccaaaatga cgcactttgc cgctctgatg cagatgtttg aacgcgtccg
     4201 ccacttcatc ggcatccatt aacgggtctg gtcggtggat taacagcaga tccagatgat
     4261 cggtcgcgag attaattagc gactgttcgg cgctcttaat gatgtgatcg cggtcagtga
     4321 tgtaatgacc aatgacgttt tcttcacgcg cggtcgtcgc gataccgcat ttactgacga
     4381 tttccatccg ttcacgcagg tgaggtgcca gtttcagtgc ctcgccaaac gccgcttcgc
     4441 actgatagcc accataaata tcagcatggt ccacggtggt cacgccgaga tccagatgct
     4501 cttcaataaa actgaccagc tggcgggcgg acatattcca gtccatcaat cgccagtagc
     4561 ccatcacaaa acgggaaaac tccgggcctt gcggcgcaat agtaatacgc tgaaccataa
     4621 tcgcttcctc ttatcagata tgagaggagt atacgcaaga ttaggttcaa aagagtgatg
     4681 gttgctccgg ttcgtctgat gacgctggct tatttgcgcg taatttgcgc attaatcgct
     4741 gccgacaaag gcgcagcacc tcttgttttt cgccatcgct cattttattc cagttaaaac
     4801 gctcatcacg actacgaaaa cagccgcgac aaaacccgcg ttcgtcagac tggcaaattc
     4861 cccggcacgg gctctggacg ggaaagaact ctaattgctc cgccacttcg ccctcctcag
     4921 ataagattat taccattatt gaagctgtta atgtccaaag tagcaacttt gcttgcacta
     4981 gaccgactgg tctactacac tccaacgcat gaacaaacac accgaacatg atactcgcga
     5041 acatctcctg gcgacgggcg agcaactttg cctgcaacgt ggattcaccg ggatggggct
     5101 aagcgaatta ctaaaaaccg ctgaagtgcc gaaagggtcc ttctatcact actttcgctc
     5161 taaagaagcg tttggcgttg ccatgcttga gcgtcattac gccgcatatc accagcgact
     5221 gactgagttg ctgcaatccg gcgaaggtaa ctaccgcgac cgcatactgg cttattacca
     5281 gcaaacactg aaccagtttt gccaacatgg aaccatcagt ggttgcctga cagtaaaact
     5341 ctctgccgaa gtgtgcgatc tgtcagaaga tatgcgcagc gcgatggata aaggtgctcg
     5401 cggcgtgatc gccctgctct ctcaggcgct ggaaaatggc cgtgagaacc attgtttaac
     5461 cttttgtggc gaaccgctac aacaggcaca agtgctttac gcactatggc tgggtgcgaa
     5521 tctgcaggcc aaaatttcgc gcagtttcga gccactggaa aacgcgctgg cccatgtaaa
     5581 aaacattatt gcgacgcctg ccgtttagca ggcatttttt atcaccagac gaccgggagc
     5641 ctttatgtca tctgaaaaac tgtattcccc actgaaagtg ggcacgatca cggcggcaaa
     5701 ccgtattttt atggcaccgc tgacgcgtct gcgcagtatt gaaccgggtg acattcctac
     5761 cccgttgatg gcggaatact atcgccaacg tgccagtgcc ggtttgatta ttagtgaagc
     5821 cacgcaaatt tctgcccagg caaaaggata tgcaggtgcg cctggcatcc atagtccgga
     5881 gcaaattgcc gcatggaaaa aaatcaccgc tggcgttcat gctgaaaatg gtcatatggc
     5941 cgtgcagctg tggcacaccg gacgcatttc tcacgccagc ctgcaacctg gcggtcaggc
     6001 accggtagcg ccttcagcac ttagcgcggg aacacgtact tctctgcgcg atgaaaatgg
     6061 tcaggcgatc cgtgttgaaa catccatgcc gcgtgcgctt gaactggaag agattccagg
     6121 tatcgtcaat gatttccgtc aggccattgc taacgcgcgt gaagccggtt ttgatctggt
     6181 agagctccac tctgctcacg gttatttgct gcatcagttc ctttctcctt cttcaaacca
     6241 tcgtaccgat cagtacggcg gcagcgtgga aaatcgcgca cgtctggtac tggaagtggt
     6301 cgatgccggg attgaagaat ggggtgccga tcgcattggc attcgcgttt cgccaatcgg
     6361 tactttccag aacacggata acggcccgaa tgaagaagcc gatgcactgt atctgattga
     6421 acaactgggt aaacgcggca ttgcttatct gcatatgtca gaaccagatt gggcgggggg
     6481 tgaaccgtat actgatgcgt tccgcgaaaa agtacgcgcc cgtttccacg gtccgattat
     6541 cggcgcaggt gcatacacag tagaaaaagc tgaaacgctg atcggcaaag ggttaattga
     6601 tgcggtggca tttggtcgtg actggattgc gaacccggat ctggtcgccc gcttgcagcg
     6661 caaagctgag cttaacccac agcgtgccga aagtttctac ggtggcggcg cggaaggcta
     6721 taccgattac ccgacgttgt aatccaacat tgcgagcggc gtaaagccgc cgctatacta
     6781 aaacaacatt ttgaatctgt tagccatttt gaggataaaa agatgcgtct tcttcatacc
     6841 atgctgcgcg ttggcgatct gcaacgctcc atcgattttt ataccaaagt gctgggcatg
     6901 aaactgctgc gtaccagcga aaacccggaa tacaaatact cactggcgtt tgttggctac
     6961 ggcccggaaa ccgaagaagc ggtgattgaa ctgacctaca actggggcgt ggataaatac
     7021 gaactcggca ctgcttatgg tcacatcgcg cttagcgtag ataacgccgc tgaagcgtgc
     7081 gaaaaaatcc gtcaaaacgg gggtaacgtg acccgtgaag cgggtccggt aaaaggcggt
     7141 actacggtta tcgcgtttgt ggaagatccg gacggttaca aaattgagtt aatcgaagag
     7201 aaagacgccg gtcgcggtct gggcaactaa tctcctgccg ggcgtgaact catcgcgccc
     7261 gcatctttac tgcatcgaca agtaatattt gtcataatgc gcgctgcaat ttatccgtat
     7321 taagagaatc agatgtccga taacgctcaa cttaccggtc tgtgcgaccg ttttcgtggt
     7381 ttttatcctg ttgtgatcga tgttgaaaca gccggattta acgccaaaac cgatgcgctg
     7441 cttgagattg ccgccatcac cctgaaaatg gatgaacaag gctggctgat gccggacacc
     7501 acattacatt tccacgtcga accatttgtc ggcgcaaatt tgcaaccaga agccctcgcc
     7561 ttcaacggca ttgacccgaa cgatcccgat cgcggcgcgg tcagcgaata cgagacgctg
     7621 cacgaaattt ttaaagttgt acgtaaaggt attaaagcga gcggctgtaa ccgcgccatt
     7681 atggtggcgc acaatgccaa ttttgatcac agctttatga tggccgccgc agaacgcgcc
     7741 tcactgaaac gtaacccgtt ccaccctttc gccacttttg acactgctgc actggccggg
     7801 ctggcactcg gacaaaccgt attgtcaaag gcttgccaga ccgctggcat ggacttcgac
     7861 agcacccagg cgcactccgc gctgtacgac accgaacgca ctgctgtgct gttttgtgaa
     7921 atcgtcaacc gctggaaacg tctgggaggc tggccgctac ctgccgccga agaggtgtaa
     7981 tcgagtcgat gcctgatgac atgcaatgat tcaggcatct atagtgaggc tattccacgc
     8041 atcctgcatg atattcacgg ggaatagcgt taatggcaga taatccagac ccttcatcgc
     8101 tcctgccgga cgtgttttca ccggcgaccc gcgactggtt tcttcgcgcc tttaaacagc
     8161 cgaccgctgt ccagtcgcaa acctggcatg tggcggcgcg aagcgaacat gcgctggtga
     8221 ttgcaccgac cggctccggg aaaacgctgg cagcatttct ctacgccctc gatcggctct
     8281 tccgcgaagg cggcgaagat acccgcgagg cgcataagcg taaaacctca cgcatcctct
     8341 atatttcacc gataaaagct ctgggcaccg acgttcagcg caacttgcag ctcccgttga
     8401 agggtattgc cgatgaacgg cggcggcgcg gcgaaacgga agtcaatctt cgcgtaggga
     8461 tccgtactgg cgatacgcct gcacaggaac gcagcaaact cacccgtaat ccgccggata
     8521 ttctgatcac cacacccgaa tcactctatc tgatgctgac ctcccgcgcg cgcgaaacgc
     8581 tacgcggcgt cgaaacggta attattgatg aagtccacgc ggtagcgggc agtaaacgtg
     8641 gtgcgcatct ggcgttaagt ctggagcggc tcgatgcgct gctccacacc tcagcacagc
     8701 gaattggcct ttctgccact gtgcgctcag ccagcgatgt ggcagcattt cttggtggcg
     8761 atcgcccggt tacggtagtc aacccgcccg caatgcgcca tccgcagata cgaattgtcg
     8821 taccggtcgc caatatggat gatgtctcat cggtcgccag cagcaccggc gaagacagcc
     8881 atgccggccg ggaaggctcc atctggccat atattgaaac gggtatcctt gatgaagtgt
     8941 tgcgccatcg ctcgaccatt gtctttacta attcacgtgg gctggcggaa aaactgacgg
     9001 cacgattaaa tgagctttac gccgcacgct tacagcgttc cccgtctatc gccgttgatg
     9061 cggcccattt cgagtcgacc tccggcgcaa cctctaaccg tgtacaaagt agcgacgttt
     9121 ttattgcccg ctcacaccac ggctccgtct ctaaagaaca acgagcaatc accgagcagg
     9181 cgctgaaatc gggtgagtta cgctgcgtgg tcgcaacctc cagtctcgaa ctggggattg
     9241 atatgggcgc agtggatctg gtgattcagg tggcaacgcc gctttctgtt gccagtgggt
     9301 tacaacgcat tggtcgcgcc ggacatcagg ttggcggtgt atctaaaggg ctgtttttcc
     9361 ctcgtacccg gcgtgactta gtcgattccg cagtcattgt agagtgtatg ttcgcaggca
     9421 ggctggaaaa cctgacaccg ccgcataatc cactcgacgt ccttgcgcag caaaccgttg
     9481 ccgccgcggc gatggaggca ttacaggtag acgaatggta ctcccgcgta caccgtgccg
     9541 caccgtggaa agatctgcca agacgtgttt ttgacgccac gctggatatg ctttccgggc
     9601 gctatccctc tggcgatttt tctgcttttc gccccaaact ggtctggaac agggagaccg
     9661 ggatattgac tgcccgacct ggcgctcaat tgttggcggt taccagcggc ggcaccattc
     9721 cggatcgtgg catgtatagc gtgttattac ccgaaggtga agaacaggcc ggttcgcggc
     9781 gggtgggtga actggatgag gagatggtat atgagtcgcg ggtgaacgac attatcactc
     9841 tcggcgctac ctcatggcgg atccagcaaa tcacccgcga tcaggtgatt gtgactcctg
     9901 ctccgggtcg ttctgcccgg ctccccttct ggcgtggtga aggtaacgga cgtccggctg
     9961 aattaggcga gatgatcggc gattttcttc atttgctggc agatggcgcg ttcttttccg
    10021 ggactattcc cccgtggctg gcagaagaaa atacgatcgc caatattcag gggttgattg
    10081 acgagcaacg caacgcgacg ggcatcgttc cggggagtcg ccatctggtc ctcgaacggt
    10141 gccgtgatga aattggcgac tggcgtatta ttttgcactc tccctatgga agacgggtgc
    10201 atgaaccctg ggcgctggcg attgccgggc gaatacatgc cctatggggc gctgacgcgt
    10261 ctgtggtcgc cagtgatgac ggcattgttg cgcgcattcc tgacacggat ggtaaattgc
    10321 ccgacgccgc gatttttttg tttgaaccag aaaagttgct gcaaattgtc cgcgaggcgg
    10381 taggcagctc ggcacttttc gccgcccgtt ttcgcgaatg cgccgcgcgg gcattattaa
    10441 tgccggggcg cactccgggc catcgcaccc cgctttggca acaacggctg cgcgccagtc
    10501 agttgctgga aatcgctcag ggatatccgg attttccggt cattctcgaa accctacgcg
    10561 aatgtctgca agatgtttat gatcttcccg cactggaacg tttgatgcgt cgcctgaacg
    10621 gtggcgaaat tcaaatatcc gatgtaacga ccactacgcc ctcgcctttc gccacaagtt
    10681 tattgttcgg ctatgtcgcg gaatttatgt accagagcga cgccccgctg gcagagcgcc
    10741 gggcatccgt actgtcgctg gacagcgagt tactgcgcaa tctactcgga caggtcgatc
    10801 cgggggaatt actcgacccg caggtcattc gccaggtgga agaagagttg caacgactgg
    10861 ctcctggcag aagagcgaaa ggtgaagaag gattgttcga cctgctgcgc gaactggggc
    10921 caatgaccgt tgaagacctg gcgcaacggc atacaggcag cagtgaagag gttgcgtcgt
    10981 atctggaaaa tcttcttgca gtaaaacgaa tcttcccagc gatgattagc ggacaggagc
    11041 gtcttgcctg tatggatgat gccgccaggc tgcgtgatgc cctcggcgta cgactaccag
    11101 agtcattgcc agagatttat ttacatagag tcagttaccc gcttcgcgat ctctttctgc
    11161 gctatctccg ggctcatgct ctggtcacgg ctgaacaact ggctcatgag tttagtctcg
    11221 gtattgccat tgtcgaagag cagcttcagc aactgcgtga acagggtctg gtgatgaatc
    11281 tgcaacaaga catctgagtg agcgatgaag tatttcgtcg tctgcgtttg cgctcgctgc
    11341 aagccgccag agaagcgacg cgtcccgttg cagccacgac ctatgcgcga ttgctgctgg
    11401 aacgtcaggg cgtattaccc gccaccgatg gtagcccggc gctctttgcc tcaacatcgc
    11461 caggcgttta tgagggcgta gatggcgtga tgcgggtgat cgaacagctt gccggagtcg
    11521 gtttacccgc ctcactctgg gaaagccaga tcctgcctgc ccgcgtacgc gactattcgc
    11581 cagaaatgct cgatgaatta ctggcaaccg gagcggttat ctggtcggga caaaaaaagc
    11641 tgggtgaaga tgacggcctg gtggcactgc atctacagga atatgccgca gaatcgttca
    11701 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    11761 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    11821 gcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    11881 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    11941 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    12001 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    12061 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    12121 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    12181 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    12241 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    12301 cgacacaagc cacgcaaacg cgccactata caccagtggc gctctctgcc aacgatccgg
    12361 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gttccaacgc
    12421 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactttat ctggcgcaag
    12481 gtggcaaaaa aatgctggtc tggcaggaaa aagaggaatt actcgcccca gaggttttcc
    12541 acgcgctgac taccgcactg cgtcgcgaac cacggctgcg ctttacgcta acagaagtga
    12601 atgatctacc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    12661 gttcgccaca agggctggat tggggataga gaaaggactg acggatgcct gttcgcatcc
    12721 gtcagtattg caggacggat tattccgcgt ccggctcttc agacttgtat ttagcggcag
    12781 tttctttgat cagctgctgc agttcgccac gctgatacat ttcgatcacg atatcacaac
    12841 cgcctaccag ctcgccgtca acccacagtt gcgggaaggt cggccagtta gcatatttcg
    12901 gcagttccgc acgaatgtcc gggttctgca gaatatcaac ataggcaaaa cgttcgccac
    12961 atgcggcaag cgcctggact gcctgggcag agaaaccgca gctcggcagt ttcggtgaac
    13021 ctttcatgta cagcaggatc gggttttcag caatctggcg ttggattttt tcgatagtgg
    13081 tgctcattgt cttgcttcct caaacttctt tacggcagta atctgacatt gtagcgggtc
    13141 agtgcggcat cggaaaataa cattttcatc acgcttttgc tattttatcc ctttgctcta
    13201 tttgttgcat ttcaaatatt cgtttcttct ctgatgccgg caaactggcg tatttataac
    13261 catttttagc tttcactgct gcattttttt gcgctcgcca acgaaacgta ttttttaaca
    13321 ataaaagcta ttaactttct cttcttctat gcattagaat catcaagttt tgtaaatcag
    13381 acgcaggcat gatagacctg ccttaacaga gggacgctca gtggcgcgga taaaccgtat
    13441 ttcgatcacg ctctgtgctt tactttttac caccctgcct ttaacgccta tggcccatgc
    13501 ttcaaagcaa gccagggaga gttctgctac cactcatatc accaaaaaag cagataaaaa
    13561 gaaaagcacg gcaaccacca aaaagaccca gaaaacagcg aaaaaagccg ccagtaaaag
    13621 tacgaccaaa agcaaaaccg cttcttccgt taaaaaatct tccattaccg cttctaaaaa
    13681 cgccaaaact cgcagcaaac acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa
    13741 gtgtaccaaa cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg
    13801 aactcttgcc gacgcgcaca aagcgaaggt gcaaaaagct acgaaagtgg caatgaataa
    13861 actgatgcag caaattggta agccatatcg ttggggtggc agctcaccgc gtaccggttt
    13921 tgattgcagc ggcctggttt attacgctta taaagatttg gtgaaaattc gtattccgcg
    13981 tacggcgaat gaaatgtatc acctgcgtga tgcagcgcca atcgaacgta gtgaactgaa
    14041 aaacggcgac ctggtctttt tccgtactca gggacgcggc acagccgatc atgtcggcgt
    14101 gtatgtcggc aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa ttcaaatcac
    14161 ttctctcagt gaagactact ggcagcgcca ctatgttggc gctcgtcggg taatgacccc
    14221 aaaaacactt cgctaaaact ttaccctgtt gttacggcaa cagggtaagt tcatcttttg
    14281 tctcaccttt taatttgcta ccctatccat acgcacaata aggctattgt acgtatgcaa
    14341 attaataata aaggagagta gcaatgtcat tcgaattacc tgcactacca tatgctaaag
    14401 atgctctggc accgcacatt tctgcggaaa ctatcgagta tcactacggc aagcatcatc
    14461 agacttatgt cactaacctg aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac
    14521 tggaagagat tattcgctgc tctgaaggtg gcgtattcaa caacgcagct caggtctgga
    14581 accatacttt ctactggaac tgcctggcac cgaacgccgg tggcgaaccg actggaaaag
    14641 tcgctgaagc tatcgccgca tcttttggca gctttgccga tttcaaagcg cagtttactg
    14701 atgcagcgat caaaaacttt ggttctggct ggacctggct ggtgaaaaac agcgatggta
    14761 aactggctat cgtttcaacc tctaacgcgg gtactccgct gaccaccgat gcgactccgc
    14821 tgctgaccgt tgatgtctgg gaacacgctt attacatcga ctatcgcaat gcacgtcctg
    14881 gctatctgga gcacttctgg gcgctggtga actgggaatt cgtagcgaaa aatctcgctg
    14941 cataataact gatggcaaat gcagcattgc ctgatgcgct tcgcttacca tgccaacgcg
    15001 gatgaggcgt taacagcgca ttcggcaaga taaatcgcac gttgtcagca actgtaacgc
    15061 agaaggttat ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa ctgtttcagg
    15121 ttgttttctg gctgacataa acaccagcaa taatgccagt cccgcgacaa tcgctcccat
    15181 caccggcaca aagctgtatc ccagcccagc ggaaattacc gcaccaccag cagctgctcc
    15241 cagcgcattt ccaagattaa aggcaccaat attaactgat gaagacagac ccggcgcttc
    15301 actggcgaca cgcatcacgc gcatctgtaa cggcggtacg accgcaaagg ttgctgcgcc
    15361 ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg ggattgccag
    15421 cataatcacc atcaacaaca acaaaaagcc tttcaacgtg ccgttaactg aacgatctgc
    15481 cagtttgccg ccgagatagt taccgataga gaatccgaca ccaatcagca ccagcattgc
    15541 cgtgacgaac actggtgttg cgtgggtaat actttgtagt accggagaga tataggtgta
    15601 gagagtaaac attgcaccag ctcccagtac cgtcgtcagc aatgcagaca gcacctgcgg
    15661 acgcattaat accgccagct cttttttcac ttcaggtcgt gcccctgcac cacctttagg
    15721 taatgagaag aacagactta ccattgaaat cactcccagc cccgccgttg ccagaaatga
    15781 catccgccag ccgatggttt cacccaacca ggtcgccgcc ggcacgccac cgatatttgc
    15841 cagggttaac cccataaaca tagtggcaac tgcgctggcc tgtttatgtt ttggcaccac
    15901 gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat
    15961 gcgtgaaagc atcagggtca tataatccgg cgcgatggcg gaaagtacgt tgccgagcgt
    16021 gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa gtagaagcgt
    16081 catcagcggc gcgccaacca ttacgccaac tgcataggca ctgattaaca ttccggcagc
    16141 gggaatcgag acatccacac cgcgcgcaat gacgggcaac aagcccattg gcgagaactc
    16201 cgttgtcccg ataccaaacg cgccaatcgc cagcgccagc aacggatagt taattttcat
    16261 gccttatctc cacctcttcg cgtcattacg cgatattcat taaagtggcg aaagcatgac
    16321 agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca cttttgccat tttgctaaca
    16381 aacaggaagg agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc
    16441 agtcaaaccc acggccacga tcaaaccgag gacgataatc gttgttacca gtgaaaattt
    16501 aaggtcggtg ctcatcaagt tttctccttt tttattacca cacaaatagt gatattacgc
    16561 atttttacac actgtgatga aaaaatctcc cgtcatttat aatgataagt gtttttacca
    16621 cttccccttt tcgtcaagat cggccaaaat tccacgctta cactatttgc gtactggcca
    16681 ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta ccgcaacact tttgttgtgc
    16741 gtaaggtgtg taaaggcaaa cgtttacctt gcgattttgc aggagctgaa gttagggtct
    16801 ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa cgagcaaacg tttccactac
    16861 aactgtgtca cacgtaatca acaaaacacg tttcgtcgct gaagaaacgc gcaacgccgt
    16921 gtgggcagcg attaaagaat tacactactc ccctagcgcg gtggcgcgta gcctgaaggt
    16981 taaccacacc aagtctatcg gtttgctggc gaccagcagc gaagcggcct attttgccga
    17041 gatcattgaa gcggttgaaa aaaattgctt ccagaaaggt tacaccctga ttctgggtaa
    17101 tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg atgatggcgc aaaaacgcgt
    17161 cgatggtctg ctggtgatgt gttctgagta cccagagccg ttgctggcga tgctggaaga
    17221 gtatcgccat atcccaatgg tggtgatgga ctggggtgaa gcaaaagctg acttcaccga
    17281 tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc gggcgttatc tgattgaacg
    17341 cggtcaccgc gaaatcggcg tcatccccgg cccgctggaa cgtaacaccg gcgcaggccg
    17401 ccttgccggt tttatgaagg cgatggaaga agcgatgatc aaggtgccgg aaagctggat
    17461 tgttcagggt gactttgaac ccgaatctgg ttatcgcgcc atgcagcaaa tactgtcgca
    17521 gccgcatcgc cctactgccg tcttctgtgg tggcgatatc atggcaatgg gcgcactttg
    17581 tgctgctgat gaaatgggtc tgcgcgtccc gcaggatgtt tcgctgatcg gttatgataa
    17641 cgtgcgcaac gcccgctatt ttacgccggc gctaaccacg atccaccagc caaaagattc
    17701 gctgggtgaa acagcgttca acatgctgtt ggatcgtatc gtcaacaaac gtgaagaacc
    17761 gcagtccatt gaagtgcatc cgcgcttgat tgaacgccgc tccgtggctg acggcccgtt
    17821 ccgcgactat cgtcgttaat cacccgttgc gggagtctct tccggctccc gcagccactc
    17881 cttattcagc gtctcactat cgcctagata ctcaagcagc caggttaacg caggcgacat
    17941 atcattttgc tgccatgtca gacaacatgc cgaatccgga aaggggtttt ccagttctaa
    18001 tgccacccac ttcccctcat taagccacgg tttggcgaaa tgtgttggca ccatccctat
    18061 gcataatcct gccgagatac aggttgccga tgattcccag tcaggcacga caactctttt
    18121 ttggttatcc agcaaccaag taatacgttt aggtagcgtt cgcgaggtgt cttcgcgcac
    18181 caacgacggc cagttgcgca acgtatcatc gctgaacggg ccatccatca acgccagcgg
    18241 gtggtggctg gcaacaacgc aactccagct tagcatcccc atatcccgga aggcataacg
    18301 accgcctacc ggaatcgcgc gtgttgcgcc aatcgccagt tccacgcgcc catcggaaag
    18361 cgcatcccag acaccgttaa acacttcctg aaagaccaga agttcgacgt cgtcaaaatg
    18421 gcgataaaaa tcaacgatca tctgccgtgt acgttctggc ctgacaatat tatccactgc
    18481 gatagctaac tgaccgcgcc agccgttcgc tatctgctga cattgctggc gggtgatctg
    18541 catttttttg acaacagagc gcccttcttt gagaaaccac gctccggcag cggtcagttc
    18601 cacatcacgg tgccgtcgtt caaagagcgg caccgccagc cactcttcca gctgacgcac
    18661 ggtatagctg accgcagaag gaacgcgatg cagctcctgt gccgcagcgc taaaactacc
    18721 attacgcgct accgcatcaa caacttcgag tgaatattct gaccacatag tctgcctgca
    18781 aaatttttga aatcagtcat caaatattac cgtttcacaa cactaatttc actccctaca
    18841 ctttgcggcg gtgtttaatt gagagattta gagaatatac atgcaacctg ggaaaagatt
    18901 tttagtctgg ctggcgggtt tgagcgtact cggttttctg gcaaccgata tgtatctgcc
    18961 tgctttcgcc gccatacagg ccgacctgca aacgcctgcg tctgctgtca gtgccagcct
    19021 tagtctgttc cttgccggtt ttgccgcagc ccagcttctg tgggggccac tctccgaccg
    19081 ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt tttgcgttag gtagtctggg
    19141 gatgctgtgg gtagaaaacg ccgctacgct gctggtattg cgttttgtac aggctgtggg
    19201 tgtctgcgcc gcggcggtta tctggcaagc gttagtgacg gattattatc cttcacagaa
    19261 agttaaccga atttttgcga ccatcatgcc gctggtgggt ctatctccgg ccctggctcc
    19321 tctgttagga agctggctgc tggtccattt ttcctggcag gcgattttcg ccaccctgtt
    19381 tgccattacc gtggtgctga ttctgcctat tttctggctc aaacccgcga cgaaggcccg
    19441 taacaatagt caggatggtc tgacctttac cgacctgcta cgttctaaaa cctatcgcgg
    19501 caacgtgctg atatacgcgg cctgttcagc cagttttttt gcatggctga ccggttcacc
    19561 gttcatcctt agtgaaatgg gctacagccc ggcagttatt ggtttaagtt atgtcccgca
    19621 aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc gcgctgcaga aatggcaagg
    19681 caagcagtta ttaccgtggt tgctggtgct gtttgcagtc agcgtcattg cgacctgggc
    19741 tgcaggcttc attagccatg tgtcgctggt cgaaatcctg atcccattct gtgtgatggc
    19801 gattgccaat ggcgcgatct acccgattgt tgtcgcccag gcgctgcgtc ccttcccaca
    19861 cgcaactggt cgcgccgcag cgttgcagaa cactctacaa ctgggtctgt gcttcctcgc
    19921 aagtctggta gtttcctggc tgatcagtat cagcacgcca ttgctcacca ccaccagcgt
    19981 gatgttatca acagtagtgc tggtcgctct gggttacatg atgcaacgtt gtgaagaagt
    20041 tggctgccag aatcatggca atgccgaagt cgctcatagc gaatcacact gatctatatc
    20101 gatatactta tacttaggct gctaacaaaa ttttgttgta tgattgaaat tagcggccta
    20161 tactaatttc gagttgttaa agctacgata aatattatgt ttttacgggg acaggatcgt
    20221 tcccgactca ctatggatgg tcatttcggc aaaggttcct cctttccctc tgttctacgt
    20281 cggattatag actcgcggtt ttttctgcga gatttctcac aaagcccaaa aagcgtctac
    20341 gctgttttaa ggttctgatc accgaccagt gatggagaaa ctatgagttc atcgtgtata
    20401 gaagaagtca gtgtaccgga tgacaactgg taccgtatcg ccaacgaatt acttagccgt
    20461 gccggtatag ccattaacgg ttctgccccg gcggatattc gtgtgaaaaa ccccgatttt
    20521 tttaaacgcg ttctgcaaga aggctctttg gggttaggcg aaagttatat ggatggctgg
    20581 tgggaatgtg accgactgga tatgtttttt agcaaagtct tacgcgcagg tctcgagaac
    20641 caactccccc atcatttcaa agacacgctg cgtattgcca gcgctcgtct cttcaatctg
    20701 cagagtaaaa aacgtgcctg gatagtcggc aaggagcatt acgatttggg taatgacttg
    20761 ttcagccgca tgcttgatcc cttcatgcaa tattcctgcg cttactggaa agatgccgat
    20821 aatctggaat ctgcccagca ggcgaagctc aaaatgattt gtgaaaaatt gcagttaaaa
    20881 ccagggatgc gcgtactgga tattggctgc ggctggggcg gactggcgca ctacatggca
    20941 tctaactatg acgtaagcgt ggtcggcgtc accatttctg ccgaacagca aaaaatggct
    21001 caggaacgct gtgaaggcct ggatgtcacc attttgctgc aagattatcg tgacctgaac
    21061 gaccagtttg atcgtattgt ttctgtgggg atgttcgagc acgtcggacc gaaaaattac
    21121 gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg aaggtatatt cctgctccat
    21181 actatcggtt cgaaaaaaac cgatctgaat gttgatccct ggattaataa atatattttt
    21241 ccgaacggtt gcctgccctc tgtacgccag attgctcaat ccagcgaacc ccacttcgtg
    21301 atggaagact ggcataactt cggtgctgat tacgatacta cgttgatggc gtggtatgaa
    21361 cgattcctcg ccgcatggcc agaaattgcg gataactaca gtgaacgctt taaacgaatg
    21421 tttacctatt atctgaatgc ctgtgcaggt gctttccgcg cccgtgatat tcagctctgg
    21481 caggtcgtgt tctcacgcgg tgttgaaaac ggccttcgag tcgctcgcta aaggctattc
    21541 tatcgccccc tctccggggg cgatttcaga tcaggcttct gtacctggtt gattcatggc
    21601 attttctcgt gccgccagca cacgttctac cgtatctacc actgcctgag tttgtggatc
    21661 aatttcaatg ttgacgcgtg cgccgagttt tttcttccca agagtcgtgc gttccagagt
    21721 ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact tcgccgacgg tcaggctaat
    21781 accgtcgatg ccaataaatc ctttgtacag gatatatttc atcaactgac tatcctggac
    21841 tttaaaccag atctggcgat tattttccga ggttaatatt ttcgccactt cagcggtggt
    21901 cataatatga cctgacatta agtgtccgcc aatttcatca ctgaatttcg ccgcacgctc
    21961 aacgtttacc caatccccca cttttaaatc gccaagattg gtaatgcgta acgtttcttt
    22021 catcaggtca aaactgacat ggttgccgtt aatttccgtc acggtcaggc agcaaccgtt
    22081 atgcgccacg gaagcacctg tttccaggcc gtccagcatg tggtcgggta actccaccac
    22141 atgcgtacga aaatttggtt tctcgtcaat cgacaccagt tttgcggtgc cctgtacaat
    22201 ccccgtaaac atacttacaa ctcctgaaat cagttaagac attctgttca gcacaatagc
    22261 aggtggcaaa cgcccttacc agtgaagggg taagaatgac tattttttca ctggagaatt
    22321 aataaatcct cgctacaata gactgaattt cccctgcttc ttctttttgc tgcccattca
    22381 ggcggctttt tagtctctca tataactaca aataaaaggt gttcacgtgc agaagtatat
    22441 cagtgaagcg cgtctgttat tagcattagc aatcccggtg attctcgcgc aaatcgccca
    22501 aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc tatagtgcca ccgacatggc
    22561 ggcggtcgct atcggtactt ctatctggct tccggcgatc ctctttggtc acggactgct
    22621 gctggcatta acgccggtta tcgcgcaatt aaatggttcc ggtcgacgtg agcgcattgc
    22681 gcatcaggtg cgacagggct tctggctggc aggttttgtt tccgtcctca ttatgctggt
    22741 gctgtggaat gcaggttaca ttatccgctc aatggaaaac atcgatccgg ctctggcgga
    22801 caaagccgtg ggttatctgc gtgcgttgct gtggggcgcg ccaggctatc ttttcttcca
    22861 ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag ccgggtatgg taatgggctt
    22921 tatcggcctg ctggtgaaca tcccggtgaa ctatatcttt atttatggtc atttcggtat
    22981 gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg gcggtgtatt gggtcatgtt
    23041 ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg cgcgatattc gtaacgaaaa
    23101 aggcaccgcg aaacccgatc ctgcggttat gaaacgactg attcaactcg gtttgccgat
    23161 tgcgctggca ctattctttg aagtgacact gtttgccgtc gtggctctgt tagtgtctcc
    23221 gctcggtatt gttgatgtcg caggacacca gattgccctg aactttagtt cactgatgtt
    23281 tgtgcttcca atgtcgctgg cggcagcggt aactatccgc gtaggttatc gtctgggtca
    23341 gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg cttatggtgg gtgtctgtat
    23401 ggcaaccctg acggccatat tcacggtttc actgcgggag caaatcgccc tgttgtacaa
    23461 cgacaatccc gaggttgtaa cgctggctgc gcatttgatg ttgctggcgg cggtatatca
    23521 gatttctgac tcaatccagg tgattggcag tgggattttg cgtggttata aagatacgcg
    23581 ttccattttc tatatcacct ttacggctta ctgggtgctg ggcttgccaa gcggctatat
    23641 tctggcactg accgatctgg tcgttaaacc tatggggcca gcaggcttct ggataggctt
    23701 tattattggc ctgacgtcgg cagccattat gatgatgctg cgtatgcggt tcctgcaacg
    23761 tctgccgtca gttatcattc tgcaacgagc atcccgctaa taaagacaag gcgcaacctt
    23821 cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg cgccaatccc gtcttcgtct
    23881 ggctgtaatt tcagagcgtt gcacagagtt tgccgactgg catctgttat ctcaacgtgt
    23941 atatccccgg taacttcccc tttcacattg atgaagtcag gtgagatttc tttcgcactg
    24001 gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca ctttcagttg cacatgcgtt
    24061 gccgcaccag tgatcagcca cagttttccg ccgttcagat tgatgttacc aacactaaaa
    24121 gtgacactgt caccttttac cgtactgccg tcgagaatca acacaccgcc ctgctgtaca
    24181 gtaatcgttg attgttgcag atctgcgcca tagccaataa tcattgaagc gccgtccagt
    24241 aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt cctctacgac aaaattttcg
    24301 tccgcgctgg tgggtgacaa caggatcact gttccacctt gtattaccgc attttccagc
    24361 gtaccggcat gcacttcagc ccgaccaccc acattcacgg acaggttttg cgtcttaccg
    24421 gaactgtaga gctgaaggcc atccgtcccc agacgataaa tggatccatt ctcaacaata
    24481 gtgtcagagt cttcaccacc gagattattc atggtgccac cagcaagcaa cgtatcactg
    24541 gctttcccgg ccagcataaa aacgccgccc tgattaatct cacaattgct ggcttcccca
    24601 cctggataca ccaacaattc accaccttca tctaccacga ccgctgaggt tgtcccatta
    24661 accaacaggc cgccctcttg atcgagaatg attttttccg cgcgatgtcc ttccagtaca
    24721 cgcaggttac cgccattttc cagcaacaaa ccgcaggcat aaccctgatc aacgctgaat
    24781 tcaccttcgg gatggcggcc attaaccgta gcgagcgtag agagactaat tgcgcctccc
    24841 tcggactgaa caatatctgt ggcaatggca ccgtcgctga ccgatagcgt gccattgtta
    24901 ccgtactgtg tgcatttggc gatcccaccg gttgccactt tcaaccagcc agcccccgtg
    24961 accacggtat tttcggctct tccgccagct tcaacaatca actcgccttc agtaagttgc
    25021 gtcgcgccaa tgacctcgcc ggtcttaaca atttgcacat tcccgtcgct catcaagttt
    25081 ttcgcatcag atcccatgat ttattccttt gctgcatctg tgtgccttta ttgctaccta
    25141 agtgtaaagg ctacggagga tttatccacg acagatttga gatggtggca aacaactctg
    25201 tttaaactct gatacacgaa ttattgggtt gtatcagatg taaatgcgat cctgaataaa
    25261 aatcgccctt gcaaatcaac aaaatatagc caatttgctt aaaagctctg caaacggtcg
    25321 aaatagcgca gaaaattacg ttttgcctct tgccaccttc ccactctgcc gctaatattc
    25381 gtccccgttg tcacctacaa cgttgcgttc atagctcagt tggttagagc accaccttga
    25441 catggtgggg gtcgttggtt cgagtccaat tgaacgcacc atcctgcgtc cgtagctcag
    25501 ttggttagag caccaccttg acatggtggg ggtcggtggt tcgagtccac tcggacgcac
    25561 cagattttct taatctggtc ttctcctttt tccctctgtt tcttctctgt atccaatacg
    25621 ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg agaatcgcta tggcaacttt
    25681 gttacaactt cattttgctt ttaatggccc atttggcgac gcaatggctg agcagcttaa
    25741 accgcttgct gagtcgatta atcaggaacc tggttttctg tggaaggtat ggacagaaag
    25801 tgaaaagaac cacgaagccg gtgggatcta tctttttact gatgaaaaaa gcgcccttgc
    25861 ctatctggaa aaacatactg cccgacttaa aaatctcggc gttgaggaag ttgtcgccaa
    25921 agttttcgat gtcaatgagc cactgagtca aatcaatcag gcaaaactcg cctgacagaa
    25981 tttaatcaag ggcggttagc gcccttttca tccctgtctg aaatttctca aattctaaaa
    26041 atctcaacca aacttatctg ataacactaa attcgaaaga atgcgtacag gtaagtaaca
    26101 atgaaaaaaa ttgctattgt gggtgccggg cctacgggga tctacacctt attctcgctt
    26161 ctacagcaac aaactccact ttctatttct atcttcgagc aggctgacga ggccggtgtc
    26221 gggatgccat acagtgatga ggaaaactca aaaatgatgc tggcaaatat tgccagtatt
    26281 gaaataccgc cgattaattg tacgtatctc gaatggctac aaaagcaaga agccagccat
    26341 ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc gtcagttttt accgcgaatt
    26401 ctgctgggcg aatatttccg cgatcaattt ttacgattag tagaccaggc acgacagcaa
    26461 aaatttgcag tggctgttta tgaatcatgc caggttaccg atctgcaaat tacaaatgct
    26521 ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga cgtttgattt agcggtgatt
    26581 gccacgggtc acgtctggcc tgatgaagaa gaagcaacca gaacgtattt tcccagtccg
    26641 tggtcaggcc tgatggaagc aaaggtcgat gcgtgtaacg tgggtattat gggaacatcc
    26701 ttgagcggac tggatgcggc aatggcagtg gctattcagc atggttcgtt cattgaagat
    26761 gataaacaac acgtcgtttt taaccgcgat aacgcaagtg aaaagctaaa tattacgttg
    26821 atgtcgcgca cgggtatttt acccgaagcc gatttctatt gccctattcc ctacgagccc
    26881 ttacacatcg tcaccgatca ggcattaaat gctgagattc aaaaaggtga agagggcctt
    26941 ttggatcggg tatttagatt gatagtagag gaaatcaagt ttgctgatcc agactggagc
    27001 caacgcatag ccttagagag cctgaatgtc gattcctttg ctcaagcctg gtttgccgag
    27061 cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc tccaggaagt cgaacgcaat
    27121 aaacgagaaa aacatactgt tccctggcgt tatgtcattc tgcgcctgca tgaagccgta
    27181 caggaaattg ttcctcatct gaatgaacac gaccataaac ggttcagtaa aggccttgcc
    27241 agggttttca tcgataatta tgcggcaatc ccttcagagt ctattcgtcg cctacttgcc
    27301 ttacgtgaag cgggaatcat tcatattctc gccctcggtg aagactacaa aatggaaatt
    27361 aatgagtcgc gcaccgtcct gaatacggaa gacaacagct actcgtttga cgtttttatt
    27421 gatgcccgcg gacagcgtcc gcttaaagtg aaagatattc ctttccctgg gctacgcgag
    27481 caattacaga aaacagggga tgaaatccct gatgttggcg aagattatac gttacagcaa
    27541 cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc cctggttgat gcacgaccag
    27601 cctttcgttc agggacttac ggcatgtgca gaaattggtg aggcgatggc tcgggcggtc
    27661 gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg attaaaggta aagctatcag
    27721 cactcaacaa actgatgctg ttcattaaga cgatagcgga ctccggttcg aatattgttt
    27781 tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt catgatatgc cagtgcggcg
    27841 cttccccctg gcccgagaat aattgagtcg accacgcccg tgctcactac cgttgaattt
    27901 tcaccgctgg caatgatgtg tacattgtcg ccgctgttgg caattctggc gtttgcgcca
    27961 aagctggcga tttgcaccag gtcaccatta ctggcgacat gacaccgttc acccaatgta
    28021 caaacgcgta ctcgcattcc ggtattcgca atccgactac tatctccagc gctgcttatc
    28081 cgcgaagaat taccggttac agcaacccgt gcgcgttcac cggagctgcc aatgtgactg
    28141 ttaaagccga cacttccaat ccgcacgcta taacccatac tggcaatttg cgcggcatac
    28201 ccagcggttg cgattcgggc gttatcctca caacagccga tgcgagcatg atcgtcgctg
    28261 cgggcaaact gactgcaaaa tggtatctct ccggggtctg tggccagttt ttgcatagag
    28321 gaaacttcct gatgcgcgaa gttttcatcc gccagccatt ttttccacgc atactcaacc
    28381 agactctccg cccagtccgt atatccttcc tcaatcagtg catgatgaat atcggcataa
    28441 ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca tcaccgctcc gatacgccat
    28501 tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc ggtgatagcc atcgaccatg
    28561 cttttaaagg tttcgtgtgg cgtacgcccc gtggtgcaaa gataaagatg accgaagata
    28621 aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca ataaccagta tcttacgcca
    28681 ggaaacacat ctcccacggc ttgcggatag agacacaaca gccccgtcaa tagtaacaac
    28741 ggcagcaatc catacatgac accaacatag gcgacctgct gtaagggatt aaatttagac
    28801 tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa tgccaaacaa ataaaatcgc
    28861 gtttgttttg ccgctcgttc cagccacccc tgacgacgaa tgcgatagtg gtgaccatta
    28921 tccccaacgg cattgatcag cacaaagccg agccagcatg ccagtaacaa aaatccgcaa
    28981 acttcatgca ccgcaaccag acttttaacc gcagttgcgc ccaccatcgc aaagtgattt
    29041 atcagcccac ttgccagcaa caatacaaag agtaacgcat tcgaccagtg ccataatctg
    29101 accgctttgc tgtaaagata aaccttctca ccatgatcgg tggctgattt cttcacgcca
    29161 cgagcacgaa gcaaggcgtg cagccccagc accaaccaca tcccaaccag caatactccg
    29221 gcaatgatca accacaccgg ccaaaattcg ggagtgaact gtggcacata gttcgctaac
    29281 tggctctgaa actgttcagc atgttgcgac gggttcatac attttccttt ttaatcaaat
    29341 gttgaccgaa ccggcgatac agatgcggtt tcccggcacc aggtagctga tattgatagt
    29401 atttattatc ctgcaaccac gcctgaattt ctgggctatc ttcgcgacca aagatcaatg
    29461 cgtgttccgg acaggcgctg acgcaaatgg gcggaaagcc ttttgccagt cgggactcag
    29521 cgcagaaatc acatttatct gccactttag tgacagggtt aaggtagcga acctggtacg
    29581 gacaagcgcc aatgcaataa ctgcagccga tgcactgcga tttttctacc cgcacgatcc
    29641 cctgctcatc acgccacgac gcccctgtcg ggcatacgtc gatgcacgga gcatcttcgc
    29701 agtgctgaca tgactggcga aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg
    29761 gaatgtgtgc tatcgataaa cggcttccct gagcggggac gtggttagtt ttacggcatg
    29821 cgcgggcgca aatattacag ccattgcacc gtgactcgtc atgaatcatg gcataccgaa
    29881 cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc agaaccagta aaaaagatta
    29941 ctgttcccat ccccagaaca aatttgcgtc gagtgaagga catctgatta tcccctaatg
    30001 cagtggctgc aactgtccac cctatccatg acatctccct gaaatttcag gggttctgaa
    30061 actgctctac ctcgaaccat tcgcataagc cataagcctt gccataggtc ataaatcgct
    30121 cgctaaacaa gtacaacacc gccgtcgatg actccacctg ccgtatatcg gcatattcag
    30181 gtgcaacatc catcgcggcg atcgcggctt caatctgcgc ttcctggaag taatatggcg
    30241 cttgcatcag catggcgact ttgtaaggac gaggatatgt ctggcactca aaacgaacgg
    30301 cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg caaagacatc gccgcgtagt
    30361 tttcactcat ggcctgcgtt gagtaatagt aatcatcctg acttcccgtc atacaagcga
    30421 tatctgcgta gtctccgttc tgcttaatgc tttccagcaa aactgacagt tcctcttctg
    30481 caaccgaata gggtggcgag agaaacacgg tgcacgccac cagctgccca gccgcagaat
    30541 gctggcgaat gaactcagcc aggatttcag caggttctgg ttcggttacc gtatcaacgg
    30601 ggtgacgcat cccctgcaac cattgccgct tcgcctcgtc aacttcgctg actttcgcta
    30661 acggcaattc atcctgatgg ttcatcttac ccattcctta cgcaggcagt agattgtgtg
    30721 ctgccagatc ggcggcaata tcttccagcc ccagacgctg caatgtttcg cgggttggac
    30781 aaccaagctg tgggtcccag cccatttctt tgtagaacat cgtgagcgaa gcatgcatat
    30841 cgtcacgatc cattttgtca gtaccttcag taaagaccgg gatctgcgga tccttgtcga
    30901 atacccagga actgataaga tcgtgttcat tacgcatatc tttggtttgc atcagtttta
    30961 ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc taaatctaat ttttcctgag
    31021 tcatctcttc gccggtgatc gctttgaaga atttggcttc aagcgccaga tcgccccggt
    31081 aattacgtct tttcagcggc gaaacggtca ttggccagac ccagttacac agggtgacgg
    31141 cgttatgcaa acagacccgc aacagcgacc acttagcata tttaattttt gcgtcattga
    31201 ttggcgtgta gtttttggtt tcatcgtagg catcttcaga accaaaaagc tctttcgcca
    31261 cttcacgttg cagtttcaat ggcaagccgg aaccaataaa gttgatatgg gtatgcgtca
    31321 tgcagtcacg gttgaacata cagttaacaa tggaaccgac ctgcgctgac gcttcattgg
    31381 catggtgaac cggatagcca aacggcgacc agagtttatt tttcgcgtag ccccagtact
    31441 cttcacccaa attccagcgt tctgcgatgg catatgaacc atcagccagg tggctcagct
    31501 cacccacacg atgcgccaga cggtagtaaa aatctttaat gaagttaacg tcacccgctt
    31561 ccagttgatc ccagcgaatt tccgcatact cttcagcagg cagaacacgc ttgaacacac
    31621 ctttgctgta acaataggta aagtcgcgat gcaactgccc gtagttacac cataggccgt
    31681 agtcatcgaa cagattcaga cccaccaggt taccaatcac acgaccatcg tctttatctt
    31741 caaaatcttt cggaccgttc gggaagatgg tggtctggac aaagttggct acacaagtgt
    31801 taccacctgt gctgggcacg ccaaactctt tcacccgagg aatattcatt tgggtcatac
    31861 aacggatcgg gcaagagtgg cagccactca tttttactgt gtatttctct gccgccggac
    31921 ctaagtcaaa aacggattta taggtacgaa agccgaccgt gttctgattg cctggcggaa
    31981 tttcccccgt ttcaatcgga ccaccttcag ccgcgcccca aaacagccct ttacgtgctg
    32041 tccaacgtga cttgggatct gaatactctg cccacgattg cggcgtactt ggcacgacat
    32101 ggttgttatt cgcaccaata agttcagtca tcatgtaatc gttgagacgc ttcatctcct
    32161 gacgatcggc aatgttgacc cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg
    32221 aacccataat tgcgccagtt cccgcaccgc cgctgtggtt acggctattc aacatgccag
    32281 agagaggaac aaggttttcc ccagcctgac caatagccgc cacacaggtt tccggactgg
    32341 tcaatcgaca aatttcttcc gtcgttgcgc gcgtcccttt tccccataag aaatcggctt
    32401 tttccaggct aactttgtca tctttaatct tcagccatac cggtgatttc gccttccctt
    32461 cgataataat gacgtcgtat ccagcgaatt tcatttgcgc tgcaaaaaag ccacccatat
    32521 gggcatcgac aactaaattt cctttggtaa aagtagaaag tgaggtgata tttacgcgag
    32581 aactacaggg ggcaccagat ccagttaatg ggccggtagc aaagactaat ttattcgctt
    32641 catcgaaagg tttcgtgcct ggcggtactt cgtcatacat aattttgtag ccgaagccca
    32701 tgccaccgac aaaactttta aacttactgg aatcttcgag ggtaatattt cctgtcgtga
    32761 gattgactct taatatatta cctgtccaac cgttagccat gattttttcc tttgcaagat
    32821 tcacacagta atatctttcc actcgataat ttttaacgcc cctgttgggc aggcgtttgc
    32881 gcattcaccg cataacacac atttcgagga ttttttactt tcggtattta cggtggccat
    32941 catccacggg catgccgtag tacaggcgct acagccaata cagcgtttat gatcgacggt
    33001 aatacagcct tctttctgct gccaggtaat cgcaccaatc gggcagacgt tcatgcattg
    33061 cggctctttg cattgacggc aggtgtccgc ggtatagttg agatcgccat acaggccgcc
    33121 gccagagcca accccgttgt cgccaaagaa ataattgcga tggattttga tacgggagaa
    33181 gaatgtccct actgagccat cgttgaagtt ggtacaagag atttcacagc ggtggcaacc
    33241 cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt gtatttatca accctactgt
    33301 gccgctatca atatcttcct gtttgcaacc caaaagtgac agtaacgcag gggcaattgt
    33361 tagaccagca aggccttttc ctgatatacg caagaattct aatcgcgtta agccaatatc
    33421 taatagtgga cgatcaaccg ggttcattta tttcgcatcc tcttcgcaaa cacagtgctg
    33481 acaattcagc agcctgatat taagcattca actaatacgc tgctgactct gtccccaaca
    33541 ccagatattt tccctaaccc gatagtgtat gagaaaagat ttaacgccga taaactaatc
    33601 ctttatggtt aggagaaaaa ataatgtgat tattctgagc ccttaacatt gatcgttatc
    33661 a
//
LOCUS       DAZFAH010000030        33235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFAH010000030 DAZFAH010000000
VERSION     DAZFAH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.030"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..770
                     /gene="elfD"
                     /locus_tag="ACHJWB_001345"
     CDS             69..770
                     /gene="elfD"
                     /locus_tag="ACHJWB_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4313001.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            795..3395
                     /gene="elfC"
                     /locus_tag="ACHJWB_001346"
     CDS             795..3395
                     /gene="elfC"
                     /locus_tag="ACHJWB_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4313002.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3386..4456
                     /gene="elfG"
                     /locus_tag="ACHJWB_001347"
     CDS             3386..4456
                     /gene="elfG"
                     /locus_tag="ACHJWB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4313003.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4468..5010
                     /gene="ycbU"
                     /locus_tag="ACHJWB_001348"
     CDS             4468..5010
                     /gene="ycbU"
                     /locus_tag="ACHJWB_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4313004.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5018..5533
                     /gene="ycbV"
                     /locus_tag="ACHJWB_001349"
     CDS             5018..5533
                     /gene="ycbV"
                     /locus_tag="ACHJWB_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4313005.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5499..6236
                     /gene="ycbF"
                     /locus_tag="ACHJWB_001350"
     CDS             5499..6236
                     /gene="ycbF"
                     /locus_tag="ACHJWB_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4313006.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6347..7357
                     /gene="pyrD"
                     /locus_tag="ACHJWB_001351"
     CDS             6347..7357
                     /gene="pyrD"
                     /locus_tag="ACHJWB_001351"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4313007.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7531..8073
                     /gene="zapC"
                     /locus_tag="ACHJWB_001352"
     CDS             7531..8073
                     /gene="zapC"
                     /locus_tag="ACHJWB_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4313008.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8070..9179)
                     /gene="ycbX"
                     /locus_tag="ACHJWB_001353"
     CDS             complement(8070..9179)
                     /gene="ycbX"
                     /locus_tag="ACHJWB_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4313009.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9423..11531
                     /gene="rlmKL"
                     /locus_tag="ACHJWB_001354"
     CDS             9423..11531
                     /gene="rlmKL"
                     /locus_tag="ACHJWB_001354"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4313010.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11543..13450
                     /gene="uup"
                     /locus_tag="ACHJWB_001355"
     CDS             11543..13450
                     /gene="uup"
                     /locus_tag="ACHJWB_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4313011.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13580..14833
                     /gene="pqiA"
                     /locus_tag="ACHJWB_001356"
     CDS             13580..14833
                     /gene="pqiA"
                     /locus_tag="ACHJWB_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4313012.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14838..16478
                     /gene="pqiB"
                     /locus_tag="ACHJWB_001357"
     CDS             14838..16478
                     /gene="pqiB"
                     /locus_tag="ACHJWB_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4313013.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16475..17038
                     /gene="pqiC"
                     /locus_tag="ACHJWB_001358"
     CDS             16475..17038
                     /gene="pqiC"
                     /locus_tag="ACHJWB_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4313014.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17294..17461
                     /gene="rmf"
                     /locus_tag="ACHJWB_001359"
     CDS             17294..17461
                     /gene="rmf"
                     /locus_tag="ACHJWB_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4313015.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17531..18049)
                     /gene="fabA"
                     /locus_tag="ACHJWB_001360"
     CDS             complement(17531..18049)
                     /gene="fabA"
                     /locus_tag="ACHJWB_001360"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4313016.1"
                     /translation="MVDKRESYTKEDLLASGRSELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18118..19878)
                     /gene="ycbZ"
                     /locus_tag="ACHJWB_001361"
     CDS             complement(18118..19878)
                     /gene="ycbZ"
                     /locus_tag="ACHJWB_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4313017.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20064..20516
                     /gene="matP"
                     /locus_tag="ACHJWB_001362"
     CDS             20064..20516
                     /gene="matP"
                     /locus_tag="ACHJWB_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4313018.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20592..21632)
                     /gene="ompA"
                     /locus_tag="ACHJWB_001363"
     CDS             complement(20592..21632)
                     /gene="ompA"
                     /locus_tag="ACHJWB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4313019.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21989..22498)
                     /gene="sulA"
                     /locus_tag="ACHJWB_001364"
     CDS             complement(21989..22498)
                     /gene="sulA"
                     /locus_tag="ACHJWB_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4313020.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22717..23346
                     /gene="sxy"
                     /locus_tag="ACHJWB_001365"
     CDS             22717..23346
                     /gene="sxy"
                     /locus_tag="ACHJWB_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4313021.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRDRLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23309..25471)
                     /gene="yccS"
                     /locus_tag="ACHJWB_001366"
     CDS             complement(23309..25471)
                     /gene="yccS"
                     /locus_tag="ACHJWB_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4313022.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLLCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRHNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25481..25927)
                     /gene="yccF"
                     /locus_tag="ACHJWB_001367"
     CDS             complement(25481..25927)
                     /gene="yccF"
                     /locus_tag="ACHJWB_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4313023.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26050..28104
                     /gene="helD"
                     /locus_tag="ACHJWB_001368"
     CDS             26050..28104
                     /gene="helD"
                     /locus_tag="ACHJWB_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4313024.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAEKAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28136..28594)
                     /gene="mgsA"
                     /locus_tag="ACHJWB_001369"
     CDS             complement(28136..28594)
                     /gene="mgsA"
                     /locus_tag="ACHJWB_001369"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4313025.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28690..29352)
                     /gene="yccT"
                     /locus_tag="ACHJWB_001370"
     CDS             complement(28690..29352)
                     /gene="yccT"
                     /locus_tag="ACHJWB_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4313026.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSLVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29525..29938
                     /gene="yccU"
                     /locus_tag="ACHJWB_001371"
     CDS             29525..29938
                     /gene="yccU"
                     /locus_tag="ACHJWB_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4313027.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29983..30300)
                     /gene="hspQ"
                     /locus_tag="ACHJWB_001372"
     CDS             complement(29983..30300)
                     /gene="hspQ"
                     /locus_tag="ACHJWB_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4313028.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30358..31548)
                     /gene="rlmI"
                     /locus_tag="ACHJWB_001373"
     CDS             complement(30358..31548)
                     /gene="rlmI"
                     /locus_tag="ACHJWB_001373"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4313029.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31643..31921
                     /gene="yccX"
                     /locus_tag="ACHJWB_001374"
     CDS             31643..31921
                     /gene="yccX"
                     /locus_tag="ACHJWB_001374"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4313030.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIH"
     gene            complement(31918..32247)
                     /gene="tusE"
                     /locus_tag="ACHJWB_001375"
     CDS             complement(31918..32247)
                     /gene="tusE"
                     /locus_tag="ACHJWB_001375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4313031.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32338..32997)
                     /gene="yccA"
                     /locus_tag="ACHJWB_001376"
     CDS             complement(32338..32997)
                     /gene="yccA"
                     /locus_tag="ACHJWB_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4313032.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT         8563 a         7940 c         8706 g         8026 t
ORIGIN      
        1 tgttttatta attagtaccc ctctagtgtt ctggagggga tattcatatt ttttaagagt
       61 gactatttat gaaaacttgc ataacaaagg gaattgtgac cgtaagttta acggcaatat
      121 tactcagctg ctcatccgca tgggcggcag gtaaaggcgg gattggactt gcagcaacac
      181 gtcttgttta ttcagaaggt gaagagcaaa tttcactggg tgtacgtaac accagtccgg
      241 atgttcctta tcttattcag tcatgggtga tgaccccaga taataaaaaa tcagcagatt
      301 ttattattac accaccgcta tttgtgctga atccggctaa tgagaatctg ttacgcatta
      361 tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct tttcttcact agcgtacggg
      421 cagtcccttc aacaacgaag cgggaagagg gaaataccct gaagattgcc acacaaagcg
      481 tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc cttaggcgag gctccggcga
      541 aactgcgttg cacttcgtca gctgacatgg ttacggtcag taacccaaca ccttatttca
      601 ttaccctgac agacctgaaa ataggtggaa aagtagttaa aaatcaaatg atttccccct
      661 ttgataaata ccaattttct ctgccaaagg gggccaaaaa tagcagcgta acgtatcgaa
      721 ccatcaatga ctacggggcg gaaacgccgc aactcaactg taaatcgtaa gccgtcttca
      781 gttaagagag cgagatgtat agaactcatc gacaacacag cctgttaagc tctggtggag
      841 tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat gcacaagctg
      901 aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc gatttgtctc
      961 gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt gttctgaatc
     1021 agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag aaggggattg
     1081 ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat gcgtttccgg
     1141 cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt ccggatgcca
     1201 gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa atcgccatca
     1261 aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc aacgcgctat
     1321 tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac agtgattctg
     1381 gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg agattgcgca
     1441 acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat ctcagcagct
     1501 atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat gattatactg
     1561 caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca gatgacaaca
     1621 tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc aaaagcaatg
     1681 cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta tcgcctggtg
     1741 cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta gttgaaatca
     1801 aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg ccattactcc
     1861 agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc aatagtaatg
     1921 aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg tggggaacga
     1981 catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt ggtctgggat
     2041 ttaaccttgg cgatttcggc gcaatttcgt tcgatgcgac ccaggcgaag agtacgctgg
     2101 cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa acgctcaacc
     2161 aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt ttctacaccc
     2221 tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt gatgatgaag
     2281 atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc aaactgcagg
     2341 tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt agccagcaaa
     2401 cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac acgcaaatta
     2461 aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa cctgatgctg
     2521 atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc agaagtaatg
     2581 atagctatac caggaaaaaa aattacgcct ggatgacctc taacaccagt atcgataacg
     2641 aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac ggtaacctga
     2701 gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt agcgccagca
     2761 tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc gataacggca
     2821 gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca cagggcatta
     2881 ccctggggca atcgctgggg gaaactaacg tgctgattgc agcaccaggc gcagagaata
     2941 ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc gttgtcccct
     3001 atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta aaacgtaacg
     3061 tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg gttctggcgg
     3121 agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag ggtataccgc
     3181 tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc attattgatg
     3241 atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact gtacgctggg
     3301 gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa caaattaact
     3361 ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat gcgtgttatt
     3421 actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg ctgcttatag
     3481 cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg gcgttaaaac
     3541 cctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc agtggaacga
     3601 atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa gtggccgatg
     3661 ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca aaattaatga
     3721 ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg cggttccttt
     3781 cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg gcggtcaacg
     3841 tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa tattgcagcc
     3901 gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg cgattatttg aatgctacaa
     3961 catacccgca ggtgattcct gcacgactac aggtacaccg gttttagtgt attacctgtc
     4021 tggtactatc aattcacttg gctcatgttc cgtcaatgcc ggagaaacaa tcgaggtcga
     4081 tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataagcctc ttggggccag
     4141 aacggcagaa cttgcaattc cagtcaggtg taacacggga aacgcggggt tagttaacgt
     4201 caacctgagt ctgacggcaa ccacagaccc cagctatccc caggcgatta agacgtcacg
     4261 tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc ctgctggtgg
     4321 aacattaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga atgtctatcc
     4381 agtcagcacg acaggtgtac caccagaaac cgggcgattt gaagccacgg caacggtgag
     4441 aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatttca gtgcgttatc
     4501 ttgttcttta gcattcttaa catccatgtc gggatggctg ggcctgaaca agttagtatg
     4561 catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag tgcattacaa
     4621 aatattcata ttggtgattt taatatcagt gattttcagg ccgcgaatac cgtaagcact
     4681 gctgctgatt tgaatatcga tatcaccggt tgtgccgctg gtattactgg cgcggacgtc
     4741 ctttttagcg gcgaggctga cacccttgcg ccgacactgc tcaaactaac tgacacaggc
     4801 ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca aagtcagcaa
     4861 gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga taacacactc
     4921 aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa tgcgacggcg
     4981 gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga taatctggat
     5041 tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg gcacagtcaa
     5101 cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca agcaaatcaa
     5161 tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg gcccggagaa
     5221 atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg acgtcacctt
     5281 ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg gtacggatgc
     5341 agccagcggg ctggcaattg cgattctgga tgatgcgaag atattgattc cgcttaacca
     5401 ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt atgcgcaatt
     5461 gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga catttgtact
     5521 tcattatgac taatacgtgg aacagattgg cgctcttgat tttcgccgtt ttatcgctgc
     5581 tggtggcggg tgaattgcgg gcaggagtgg tggtcggcgg aacgcgattt atctttccgg
     5641 cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc tggcttatta
     5701 acagtaaaat caaccgccca acgcgttggg ccggggggga agcgtcgaca gtgccagcac
     5761 cattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc acgttgcgct
     5821 tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt gagttaagca
     5881 ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg atgcgctcgg
     5941 tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa gcttatcaac
     6001 aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg ccttactaca
     6061 ttaacctgat tcagttgagc gtaaatggta aagcgttaag taatgcggga gttgtgccgc
     6121 ctaaaagcca gcgtcagaca agctggtgtc aggcaatcgc accgtgtcat gtcgcctggc
     6181 gggcgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg ccctgaaaca
     6241 ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg tccgaactcg
     6301 cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt actacccctt
     6361 cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta cttttcagca
     6421 attacgccgt attacaggaa cgccgtttga agcactggtg cggcagaaag tgcctgcgaa
     6481 acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag ccggtcttga
     6541 taaagacggg gagtgcattg acgcgttagg cgcgatggga tttggatcga tcgagatcgg
     6601 taccgtcacg ccacgtccac agccaggtaa tgacaagccg cgtctctttc gtctggtaga
     6661 tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata acctcgtaga
     6721 gaacgtaaaa aaggcccatt atgacggcgt cctgggtatt aacatcggca aaaataaaga
     6781 tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa tctatgccta
     6841 tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac gcacgctgca
     6901 atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa atgatttgca
     6961 agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc tttctgaaga
     7021 agaattgatc caggttgccg atagtttagt tcgccataat attgatggcg ttattgcaac
     7081 caataccaca ctcgatcgtt ctcttgttca gggaatgaaa aattgcgatc aaaccggtgg
     7141 cttaagtggt cgtccgcttc agttaaaaag caccgaaatt attcgccgct tgtcactgga
     7201 attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta tcgctgcgcg
     7261 tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta tttttaaagg
     7321 tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa ttcctcttta
     7381 taagataatc agggctttat tttcagccct ggttgtttta tattcatcgc tgttgcttat
     7441 ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg gattgtgttg
     7501 ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga taactggcgt
     7561 tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg tatgctattt
     7621 cgctcacgtt ttgcgcgcaa gatgttgact ccagatgcct tttctcccgc aggcttttgc
     7681 gttgacgacg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt taatttatct
     7741 aaagaacaaa aagccgaact ggtgctgaat gcactggtag caattcgcta tctcaaaccg
     7801 caaatgccga aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc catgccgggc
     7861 gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct ggtcgttgaa
     7921 tctggcgaaa atgccgcact atgcctgctg gcacaaccct gcgttgttat tgcgggtcgc
     7981 gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc gcaggttaat
     8041 gttgacagct tcagcctcga acaggcagtc taacgcgcca acttaagtgc agtcttcggt
     8101 acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt cagcggcgtg
     8161 acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa
     8221 gggatacgaa ttccctgatt ttctaattgc tccagcaaca cctgttggtt atttccacga
     8281 aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt gatgttggcg
     8341 gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag aatttccacc
     8401 tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc aaaatcgaca
     8461 tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc gcctgccgga
     8521 tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca aggtttaacc
     8581 acatcaaaca ccacatcacc aatgcgaatc actttccagc gatcttcttc ccaggctgac
     8641 gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc cggacaacgt
     8701 tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc atcagcaaat
     8761 gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc cacccagcgt
     8821 aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc tggcgcaatt
     8881 cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca
     8941 aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc atgcacaggt
     9001 gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt accgtcaggt
     9061 tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag agcatgtgta
     9121 agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa tgtcgccacg
     9181 gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct tattagctat
     9241 aatgcgcaac aattttctta gcgcctgtcc cattaggcta ttttatttgt cagtttggcc
     9301 ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc gcgctgtcca
     9361 tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt aaaagtgacg
     9421 atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta aaaactgaac
     9481 tggaaaacct gggggccgtt gaatgccagg tggttcaggg tggggtccat ttcaagggcg
     9541 acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt attatgttgc
     9601 cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt caggcgatca
     9661 actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt ggtttgaatg
     9721 acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc gtcgatgctt
     9781 tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta
     9841 acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt gatggtttac
     9901 atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc ctggcagccg
     9961 ctattgtgat gcgatccggc tggcagccag gaacaccgct gctcgatccg atgtgtggtt
    10021 ccggtacgtt gctgattgaa gcggcgatgc tagcgaccga tcgcgcacca ggcttgcacc
    10081 gtgggcgttg gggctttagc ggctgggtgc agcatgatga agatatctgg caggaagtga
    10141 aagcggaagc gcaaactcgt gcccgtaaag gcctggctga gtatagctct catttttacg
    10201 gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt cttgcgggga
    10261 ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat ccgctgccga
    10321 aagggccgta cggtacagtg ttgagcaacc cgccatacgg ggaacgtctg gacagcgaac
    10381 cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag ttcggtggct
    10441 ggaatctctc tttgtttagc gcctcgccgg atctgctcag ctgcctgcag ctgcgtgcag
    10501 acaaacagta caaggcgaaa aacggcccgc tggactgcgt gcagaaaaac taccacgttg
    10561 ccgaaagcac tccagacagc aaaccggcaa tggtggcgga agactacgcc aaccgcctgc
    10621 gtaagaacct caaaaaattc gagaagtggg ctcgtcagga agggattgaa tgttaccgcc
    10681 tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttatgcc gactgggtgg
    10741 tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt cagcgtctgt
    10801 tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa ctggtactga
    10861 aaacccgtga acgccagaag ggcaaaaacc agtaccagaa actgggcgag aagggcgagt
    10921 ttctggaagt taccgaatat aacgctcact tgtgggtgaa tctgacggat tacctcgata
    10981 ccggtctgtt cctcgatcac cgtatcgccc gtcgtatgct cggccagatg agcaaaggca
    11041 aagatttcct caacctgttc tcttataccg gcagcgccac cgtgcatgcg ggattaggcg
    11101 gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggagtgg gcagaacgca
    11161 acctgcgtct gaatggcctg accgggcgtg cgcatcgcct gattcaggcc gattgcctgg
    11221 cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg ccaaccttct
    11281 ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg gcgctgatga
    11341 aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac aacaaacgtg
    11401 gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa gaaattacgc
    11461 aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc tggctgatta
    11521 ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcct ggctgtcgtt
    11581 cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataatg aacgtgtttg
    11641 tctggtaggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca accgtgaaca
    11701 agggctggat gacggtcgca ttatttacga gcaagatttg attgtagctc gtctgcaaca
    11761 ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag gcattgaaga
    11821 acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga acgacccgag
    11881 cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc acaacttgtg
    11941 gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc ctaatgttgc
    12001 gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg cgctggtgag
    12061 caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg aaaccatcga
    12121 ctggctggaa gggttcctta aaaccttcaa tgggacgatt attttcatct cccacgaccg
    12181 ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca agctggtgac
    12241 ctatccgggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc gcgtggaaga
    12301 gctacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaagtgtgga ttcgccaggg
    12361 gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag caatgcgtcg
    12421 cgagcgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg aagaggccag
    12481 ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggttg acggtaagca
    12541 actggtgaaa gatttttcgg cccaggttct acgtggcgac aaaattgccc tgattggtcc
    12601 gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc aagcggacag
    12661 cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc accgcgcgga
    12721 actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag aggtgatggt
    12781 taacggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc atccgaaacg
    12841 ggcgatgacg ccggtacgtg cgctttctgg cggtgagcgg aaccgcttgc tgctggcgcg
    12901 tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg atcttgatgt
    12961 cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg tattgctggt
    13021 tagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct tcgaaggcgg
    13081 cggtaaaatt ggtcgttatg ttggcggtta tcatgatgcc cgtggtcagc aagaacaata
    13141 tgtggcactc aaacagcctg cggtgaaaaa aagcgaagaa gccgccgcgg caaaagcaga
    13201 aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac tggagcagct
    13261 accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc aagtggcgga
    13321 tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgcttgctg atatggctgc
    13381 tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag cgttaaaaaa
    13441 tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg taaaattgcc
    13501 tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct gtaaaatagc
    13561 gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc
    13621 gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc
    13681 ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc ccaccgccta
    13741 tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg tgaatatgaa
    13801 tgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc ttttttctga
    13861 ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc ccgcgttttg
    13921 tctgataacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt taaaagagca
    13981 actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt tcctcgctgg
    14041 tgtgctggtc agtttcgtta aactgatggc ttacggcagc attggggtag gcagcagctt
    14101 tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg ttgatcgtcg
    14161 ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa aaccaggcgt
    14221 cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc ttcctgctga
    14281 tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa acagcctgca
    14341 gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta atattttgcc
    14401 catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg ctggggtcat
    14461 tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg ccagtattat
    14521 ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca aagggcatgg
    14581 caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg taggccgctg
    14641 gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt
    14701 aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca taatgacaat
    14761 gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg aatcagagca
    14821 tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag aaagtgaaga
    14881 actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc tgggttcttt
    14941 tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat gcggaaggaa
    15001 ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg
    15061 ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat tccggtatgg
    15121 aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt ggtcgcgaag
    15181 ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag ccaggcgcga
    15241 aaggcagcaa aatggataaa tacgatttgc tggactcgcc accgttggcc ccgcctgatg
    15301 cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg ccaggagatc
    15361 cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc gatacacaaa
    15421 aacgcaatat cagctatcaa ctgttcatca atgcacctta tgaccgactg gtgaccaaca
    15481 atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagca gggatgcgtg
    15541 tggagatggg ctcattgaca acgctgctga gtggcggtgt cagctttgat gtgccggaag
    15601 gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat gatgatcaga
    15661 agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc tttaaagatt
    15721 cggtacgcgg tctgcaaccg ggagctccgg tagagttccg gggtattcgc ctgggtaccg
    15781 taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat gattaccgta
    15841 ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc gaaaatgcgg
    15901 atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg ctgaaaaccg
    15961 gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat acgcctgcaa
    16021 taaccggtat tcgtgaattt aatggttatc agattatccc gaccgttagc ggcggcctgg
    16081 cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg ccattgaatc
    16141 cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc
    16201 aaacgacgct ggatagcatg aacaagatcc tcgctagcca gtcgatgcag cagttgccga
    16261 cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc cagcctggct
    16321 ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg ttgcgagaac
    16381 tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt gaagcgaagg
    16441 acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag tgacgattgc
    16501 agcactgtgg ctggtcggat gcagctccgg cgaaattaat aaaaactatt accagttacc
    16561 tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt tatgggtaga
    16621 gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa ccagtgatgt
    16681 gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac agttgcgcaa
    16741 caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct cccagcctct
    16801 gggaagcgcc caggacacgc tcaatgttac cgtaacggag tttaacggtc gctatgatgg
    16861 caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga tcaaacgtcc
    16921 gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta aagtgctggc
    16981 cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc taccttaatt
    17041 ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt ttttgtttct
    17101 aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg cccgtgtagc
    17161 tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat tcgcggtatt
    17221 gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga aaccagtatg
    17281 agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg
    17341 ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca
    17401 aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag taatggcctg
    17461 attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt gatactctgt
    17521 ctgattataa tcagaaggca gacgtatcct ggaacagacc gactttcagg tcgctggcgg
    17581 tatagatcag acgaccatca accagcactt cgccatccgc caggcccata atcagacgac
    17641 ggttaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc ggcagtacct
    17701 gaccagtgaa tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc
    17761 agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat cccggcataa
    17821 ccggatcgcc aataaagtgg catccgaaga accacagatc cggattgata tccagttctg
    17881 cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc acacggtcca
    17941 tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt tcactgcgac
    18001 cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg ttctctgtaa
    18061 gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca agtccgatca
    18121 gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac gtccttcctg
    18181 ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc
    18241 atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta ctgcccagat
    18301 ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta aatggcgaac
    18361 gttagctgtg gggataatga caccttgttt cccggttaac tcacgttgct ggcaaatagc
    18421 aaagaagcct tcgattttct catttaaacc accgaccggc tgggcgcgac cgaactgatc
    18481 gactgaacct gtgatagcga tactctgatt caccggcaca tcggcgaggg cgcttatcag
    18541 ggcgcagagt tcagccatcg aggcactatc tccatcaact tcactgtatg actgctcaaa
    18601 tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca tcaggaacgc
    18661 ttgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt tgcgttcgat
    18721 gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag gttcgccaaa
    18781 agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc
    18841 gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac gttcagcgag
    18901 gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc cggagaaggt
    18961 gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact gcgggctaag
    19021 cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta ccggccaggc
    19081 atccgctccc ggtgcaggta agtgattatg tctggcggta aatgtcaccc agcgacacca
    19141 ctgggttact gactccgcat cgacaatctg cagagtatct tcaaattcgc tataaatagc
    19201 ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc gttcgcctac
    19261 cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag ggcgcgactc
    19321 atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc gcatccacag
    19381 cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat ttgcctgatg
    19441 caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc caaagagttg
    19501 ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc
    19561 actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgac cgcccaccag
    19621 ttggcctgca tcgctttgta gcgtacgcgc ggcattggca ataagattca gatactcaga
    19681 ctcttccggg gccttcgcca gcataaaaga ggaggatgct cgcgtatgca gcaactgctc
    19741 cagtgcgaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa
    19801 atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt cacgccatgc
    19861 aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc
    19921 gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcctggcat gggcgttaaa
    19981 gctcacagga tggagattct tttcttcact ggcctaaaaa gctgatattc tgtaaagagt
    20041 tacacggtaa cattgagatc gctatgaaat atcaacaact tgaaaatctt gaaagcggtt
    20101 ggaaatggaa gtatctggtt aagaagcatc gcgaagggga gttaatcacc cgttacatag
    20161 aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc
    20221 tggtaaatgg ctggattgac aagcatatga atccggaact ggtcaatcgc atgaagcaaa
    20281 ccattcgggc aagacgaaag cgccatttta atgcggaaca ccagcatacg cgcaaaaaat
    20341 cgatcgatct ggaatttatt gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta
    20401 aaacgctttc tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg
    20461 cgaataaaat gtcttctctg aagcaggatc tgcaggcatt gctgggtaag gaataactga
    20521 cgaaagtcag ttcaatttac taaaggcaaa aaaaaccccg cagcagcggg gtttttctac
    20581 cagacgagaa cttaagcctg cggctgagtt acaacgtctt tgataccttt aacttcgatc
    20641 tctacgcgac gatccggagc caggcagtcg atcagtgcag cacgctgttt cacgttgtca
    20701 caggtgttgc cagtaaccgg gttggattcg cccataccac gtgcggagat cttgtctgcc
    20761 gggatacctt tggagatcag gtaatcaaca acagactgag cacggcgctc ggacagaccc
    20821 tggttgtaag cgtcagaacc gatgcggtcg gtgtaaccca gaacaactac ggaaccgtct
    20881 ttcggatcca ggttgctcag ctggctgtac agctgatcca gagcagcctg accttccggt
    20941 ttcagggttg ctttgttgaa gttgaacaga acgtcagact tcagagtgaa gtgcttggtc
    21001 tgtacttccg gtgccggagc tggagccgga gcaactactg gagctgcttc gccctgaccg
    21061 aaacggtagg aaacacccag gctcagcatg ccgttgtccg gacgagtgcc gatggtgtgt
    21121 gcgtcaccga tgttgttggt ccactggtat tccagacggg tagcgatttc aggtgtgatc
    21181 gcgtactcaa caccgccagc gaagaccgga gaaacgccgg tgtcgtggtt tttaccataa
    21241 acgttggatt tagtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccagg
    21301 tcgtcagtga ttgggtaacc cagtttagcg gtcagttgaa cgccctgagc tttgtatgca
    21361 ccgttttcaa cgctgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaag
    21421 ccaacatacg ggttaacctg gtaaccacca aaagcaccag cgcccagttg gttttcatgg
    21481 gtcgggccat tgttgttgat gaaaccagtg tcatggtact gggaccagcc cagtttagca
    21541 ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt
    21601 gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata
    21661 cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa
    21721 tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    21781 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    21841 aaggtatgtt taatcttttt tgtcagcgac aatttacaga agagaatcgc ggaaaccgct
    21901 tcagacaagc ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa
    21961 aaagttccaa gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc
    22021 cggaaagttg tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag
    22081 cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg
    22141 ccaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca
    22201 cagtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg
    22261 cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt
    22321 gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt
    22381 cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa
    22441 ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa
    22501 tcaatccagc ccctgtgagt tactgtatgg atgtacagta catccagtga caacaaagat
    22561 caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat
    22621 taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga
    22681 atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg
    22741 atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt
    22801 ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg
    22861 tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg
    22921 acatataaaa agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta
    22981 tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa
    23041 gagaaaagca cgcgcaatac ccgggataga ctgaaagatt tgcccaatat gtcttttcat
    23101 ctggaagcga ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg
    23161 gcaaaaatgt gttggttgcg actgcgtcag caaaacagtc tggtgacaga aaagattctg
    23221 tttatgcttg aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc
    23281 caggagcttg cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt
    23341 gagtaatctg gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt
    23401 gttgtacgac cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt
    23461 tcaggctagc taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt
    23521 catcaacata gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct
    23581 cccggtgagc accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc
    23641 gaaacgcggc ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca
    23701 ccgacgccag ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac
    23761 ggttatcacg cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct
    23821 ctgtggcgcg ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc
    23881 tcactgccgc ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta
    23941 cttcaaaacc ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg
    24001 ttgcatgagc gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca
    24061 gcaccagctg cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac
    24121 ctaccagcgt accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct
    24181 ggcagacaaa caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatttgaa
    24241 tgatggcgta gccgaagcac aacaccagcg acattcttat cgcatgacgg aagagggcgg
    24301 attccggcgt gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt
    24361 catcagcgag ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa
    24421 ttgttgccag ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga
    24481 gtaaatcggc gggtgcgccg ttatggcgca tccgctccag cgcagcatca atatgcgtaa
    24541 aagcgcgctc aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca
    24601 gttgctggca cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc
    24661 tgtggcgaaa atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt
    24721 gaatatcctg tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat
    24781 cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac
    24841 cgttggcgag agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa
    24901 acatgcgcga cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt
    24961 cctgcagcgg gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga
    25021 cggcaccggc cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca
    25081 acatagtgta aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca
    25141 gaccgccgag caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg
    25201 gccagggaaa cagcaattct actgaggccg aggcgataaa aaagcataac agcgtaatga
    25261 tgaggttacg caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca
    25321 tccccagcgt tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg
    25381 ttccacaaag cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc
    25441 gtttgagcaa aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg
    25501 cattggcttc acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc
    25561 atagtgcaat agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa
    25621 tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca
    25681 gccagaaaat attcaatacc gtaccgccag tattcagcag tacatttttg ccagccgggt
    25741 tcagttcatc gacatgaata gcttcattgc cataaggcac cagagacagt ttagtgatct
    25801 cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca
    25861 acagccagcc cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacag
    25921 tacgcataaa acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt
    25981 ttgggctgga gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc
    26041 tacagagtca tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac
    26101 gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt
    26161 attcctttca atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg
    26221 gaatttgtac tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg
    26281 cagcgttttt accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa
    26341 attgcgtctg gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat
    26401 aaatggctga cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg
    26461 gcgttgccgt tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt
    26521 aaatgtcagg cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat
    26581 accgaagcca tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg
    26641 aatccggcgc aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt
    26701 gcaggaagcg gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt
    26761 gaagcgtccc ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg
    26821 gacgagcgga ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg
    26881 ctggcgctgc atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa
    26941 aatgataccg ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa
    27001 aagaaagcgc aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg
    27061 ccagaaggta acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat
    27121 cgctgggtaa gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca
    27181 cccgaagaga ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa
    27241 gcctggaaag gtgcgctgaa ggcagaaaag gctgtcgatt tttcgggcct tattcaccag
    27301 gcgattgtga ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat
    27361 gaatttcagg atatctcgcc gcagcgcgca gcgttgttag cggcattacg caagcaaaac
    27421 agtcagacga cgttgttcgc cgttggtgac gactggcagg cgatttaccg attcagcggt
    27481 gcgcaaatgt cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat
    27541 ttagacacga cttaccgttt taacagtcgt atcggtgagg tggcaaaccg gtttattcag
    27601 cagaacccag gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc
    27661 gtcacgttat tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc
    27721 aaaccggaag agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg
    27781 gaaaaagcgg caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc
    27841 aaagggcaac aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt
    27901 ccggctgcgg cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc
    27961 ccggacgctg aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta
    28021 tgggcactgt ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat
    28081 gtgccggtgg cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact
    28141 tcagacggtc cgcgagataa cgctgataat cggggatcag aatatcgacc gcgtcgttga
    28201 aatgcggcga ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc
    28261 ataccgtcgc cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg
    28321 gatcccagaa gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct
    28381 ggtcaccccc cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa
    28441 ttaagttacc ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc
    28501 gttccaccca gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt
    28561 tccgcgcagg taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct
    28621 acagattact gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc
    28681 acaaaaatct caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg
    28741 tgggtcagcc agtttaaacc aatacttcag ccgttgttct gtgagcacca gagactgcgg
    28801 tggaatagca tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt
    28861 ggcaaattgc ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc
    28921 ataatcaatc gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg
    28981 cgtcgcatcg ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg
    29041 ctcattctcc aggcgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact
    29101 gaccaccagc ggtggggaga tatacagccg ttcttcacta ttggaaagat gaatcgtctt
    29161 ctcaacgcga aacactaact gatgcggtcc attatccagt tcaatgctgt ccgcaccgcg
    29221 cagcagagag ctggaaacct tcttgccgtc gagtaccaga agatcgacat cggttgaaag
    29281 ccgcagcgtg gtggcaaaaa cggataccgg caaacataat gcaatcaagg tggtcacgat
    29341 gccggttttc ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt
    29401 ttctttaatc atacttacta acatatagac atatttcccg cttttgctct cattcattcg
    29461 tattagctgc atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga
    29521 aataatgaaa gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt
    29581 gggggcaagc gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca
    29641 ggggtatcac gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca
    29701 ggggtatggc acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa
    29761 ttcagaagcg gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg
    29821 gatgcaactg ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa
    29881 tgtggtgatg gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa
    29941 aatccccgga aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg
    30001 ggcctggagt tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg
    30061 ttcatcctgc aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc
    30121 gttatcgtcc tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc
    30181 cagctcatca ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc
    30241 gagataacct aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat
    30301 agtcacctcc cgggaaattt ttacctttga tataagggta aaattccaca tttcaagtta
    30361 catgacgcga caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg
    30421 atgatcggct gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc
    30481 ggcatccgcg atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt
    30541 caggagaata ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc
    30601 acgacacgcg cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat
    30661 aacgtcaaat ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg
    30721 gacaaactca gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc
    30781 cagcgcttcc tgggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga
    30841 tacggcgaaa ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg
    30901 gcgggtagcc aggcggctat cacgctggtc caggtagtag cccgttttgt gtccgtgctg
    30961 aatatccacc agcagtttca tgccgtgttc ttcaatcggc agcagggcag gtggcaactc
    31021 gccggtgacg aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct
    31081 gcgatcgtaa atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg
    31141 ctgatattct gcgccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat
    31201 agtaataccc ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc
    31261 tttttgcgcc agccagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc
    31321 aatgtcgata gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg
    31381 cgaataagcg ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc
    31441 gaggctggct ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg
    31501 aagtaatgat ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc
    31561 tgtcgatgca aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc
    31621 attaaggata aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg
    31681 agttcagggc gtaggatttc gctacaccac gcagtacgaa gcgaaaaaac tgggcttaac
    31741 cgggtacgcc aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg
    31801 gcaggtggaa aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtgga
    31861 acgggtgctt agcgagccgc atcatccctc gggggaatta gccgattttc gtatccatta
    31921 aatacatttt accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt
    31981 cggaaacagt cgatataaat agcggctgtt acctttttct tcgccaaatt tattcgccat
    32041 cgcttttacc agcatacgaa tcgccggaga agtattgaat tccagataga aattacgcac
    32101 aaaacgcacc acttcccagt gttctggcga cagcgaaatc ccttcgttct ctgcaatcac
    32161 cacagccagc ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc
    32221 tatctcttta ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt
    32281 gtaacaaaaa acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta
    32341 atcgcggctg gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac
    32401 atacaggcta accgtggcac gaatatagtt cgtttcaccg ccatgaatga tgttgctggt
    32461 ttcaaacaaa atagcgccag aggagatcaa aatgaagacc gcgctgatcg ccagatgcag
    32521 agcaggcagc tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc
    32581 catcagcata ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca
    32641 gcagaagaac actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc
    32701 agacagatag gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa
    32761 tgcggagata atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata
    32821 catacccacc agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt
    32881 tgcggtaatc gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac
    32941 cttatgggtg ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga
    33001 gtcactctct atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc
    33061 cagaagtttt acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag
    33121 gtcattaagc aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca
    33181 tttagctaac cccttatctt atttggcgga agcgcagaga ttcgaactct ggaac
//
LOCUS       DAZFAH010000031        32901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFAH010000031 DAZFAH010000000
VERSION     DAZFAH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.031"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHJWB_001377"
     CDS             complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHJWB_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4313033.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            369..1085
                     /gene="arcA"
                     /locus_tag="ACHJWB_001378"
     CDS             369..1085
                     /gene="arcA"
                     /locus_tag="ACHJWB_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4313034.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACHJWB_001379"
     CDS             complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACHJWB_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4313035.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACHJWB_001380"
     CDS             complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACHJWB_001380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4313036.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACHJWB_001381"
     CDS             complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACHJWB_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4313037.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACHJWB_001382"
     CDS             complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACHJWB_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4313038.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5365..6234
                     /gene="robA"
                     /locus_tag="ACHJWB_001383"
     CDS             5365..6234
                     /gene="robA"
                     /locus_tag="ACHJWB_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4313039.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACHJWB_001384"
     CDS             complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACHJWB_001384"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4313040.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEES
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6930..7442
                     /gene="yjjX"
                     /locus_tag="ACHJWB_001385"
     CDS             6930..7442
                     /gene="yjjX"
                     /locus_tag="ACHJWB_001385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4313041.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7484..7810)
                     /gene="trpR"
                     /locus_tag="ACHJWB_001386"
     CDS             complement(7484..7810)
                     /gene="trpR"
                     /locus_tag="ACHJWB_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4313042.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7900..9837)
                     /gene="sltY"
                     /locus_tag="ACHJWB_001387"
     CDS             complement(7900..9837)
                     /gene="sltY"
                     /locus_tag="ACHJWB_001387"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4313043.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10048..11715
                     /gene="ettA"
                     /locus_tag="ACHJWB_001388"
     CDS             10048..11715
                     /gene="ettA"
                     /locus_tag="ACHJWB_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4313044.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12022..13254)
                     /gene="nadR"
                     /locus_tag="ACHJWB_001389"
     CDS             complement(12022..13254)
                     /gene="nadR"
                     /locus_tag="ACHJWB_001389"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4313045.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13275..14657)
                     /gene="radA"
                     /locus_tag="ACHJWB_001390"
     CDS             complement(13275..14657)
                     /gene="radA"
                     /locus_tag="ACHJWB_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4313046.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14706..15674)
                     /gene="serB"
                     /locus_tag="ACHJWB_001391"
     CDS             complement(14706..15674)
                     /gene="serB"
                     /locus_tag="ACHJWB_001391"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4313047.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15780..16424
                     /locus_tag="ACHJWB_001392"
     CDS             15780..16424
                     /locus_tag="ACHJWB_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO4313048.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16452..17468
                     /gene="lplA"
                     /locus_tag="ACHJWB_001393"
     CDS             16452..17468
                     /gene="lplA"
                     /locus_tag="ACHJWB_001393"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4313049.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17500..17763
                     /locus_tag="ACHJWB_001394"
                     /pseudo
     CDS             17500..17763
                     /locus_tag="ACHJWB_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17924..18643)
                     /gene="deoD"
                     /locus_tag="ACHJWB_001395"
     CDS             complement(17924..18643)
                     /gene="deoD"
                     /locus_tag="ACHJWB_001395"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4313050.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18723..19946)
                     /gene="deoB"
                     /locus_tag="ACHJWB_001396"
     CDS             complement(18723..19946)
                     /gene="deoB"
                     /locus_tag="ACHJWB_001396"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4313051.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGCYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19998..21320)
                     /gene="deoA"
                     /locus_tag="ACHJWB_001397"
     CDS             complement(19998..21320)
                     /gene="deoA"
                     /locus_tag="ACHJWB_001397"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4313052.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21447..22226)
                     /gene="deoC"
                     /locus_tag="ACHJWB_001398"
     CDS             complement(21447..22226)
                     /gene="deoC"
                     /locus_tag="ACHJWB_001398"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4313053.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22484..24034
                     /locus_tag="ACHJWB_001399"
     CDS             22484..24034
                     /locus_tag="ACHJWB_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO4313054.1"
                     /translation="MPTTHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24006..24869
                     /locus_tag="ACHJWB_001400"
     CDS             24006..24869
                     /locus_tag="ACHJWB_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4313055.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(24982..25764)
                     /locus_tag="ACHJWB_001401"
     CDS             complement(24982..25764)
                     /locus_tag="ACHJWB_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4313056.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(25761..26834)
                     /locus_tag="ACHJWB_001402"
     CDS             complement(25761..26834)
                     /locus_tag="ACHJWB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4313057.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGLYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(26956..27117)
                     /locus_tag="ACHJWB_001403"
     CDS             complement(26956..27117)
                     /locus_tag="ACHJWB_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4313058.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27244..27849)
                     /gene="osmY"
                     /locus_tag="ACHJWB_001404"
     CDS             complement(27244..27849)
                     /gene="osmY"
                     /locus_tag="ACHJWB_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4313059.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28242..29831)
                     /gene="prfC"
                     /locus_tag="ACHJWB_001405"
     CDS             complement(28242..29831)
                     /gene="prfC"
                     /locus_tag="ACHJWB_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4313060.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(29922..30599)
                     /gene="yjjG"
                     /locus_tag="ACHJWB_001406"
     CDS             complement(29922..30599)
                     /gene="yjjG"
                     /locus_tag="ACHJWB_001406"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4313061.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30614..31060)
                     /gene="rimI"
                     /locus_tag="ACHJWB_001407"
     CDS             complement(30614..31060)
                     /gene="rimI"
                     /locus_tag="ACHJWB_001407"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4313062.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31029..31442)
                     /gene="holD"
                     /locus_tag="ACHJWB_001408"
     CDS             complement(31029..31442)
                     /gene="holD"
                     /locus_tag="ACHJWB_001408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4313063.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31545..32576
                     /gene="rsmC"
                     /locus_tag="ACHJWB_001409"
     CDS             31545..32576
                     /gene="rsmC"
                     /locus_tag="ACHJWB_001409"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4313064.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7746 a         9032 c         8412 g         7711 t
ORIGIN      
        1 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
       61 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
      121 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
      181 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
      241 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
      301 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
      361 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
      421 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
      481 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
      541 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
      601 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
      661 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
      721 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
      781 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggagagc
      841 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
      901 aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
      961 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     1021 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
     1081 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
     1141 aaccttattt ccagatacgt aattgatcgt ccattgtaac ttctttactg gctttcattt
     1201 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
     1261 ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg agcagtcccc
     1321 acatcacacc atccagcaac aacagcgcga gggtaaacaa catgctgttg cgccagcctt
     1381 tcaataccgc ctgcagataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
     1441 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
     1501 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
     1561 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
     1621 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt
     1681 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
     1741 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
     1801 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
     1861 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
     1921 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
     1981 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
     2041 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
     2101 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
     2161 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
     2221 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
     2281 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt
     2341 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
     2401 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
     2461 gggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag
     2521 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
     2581 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
     2641 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
     2701 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
     2761 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
     2821 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
     2881 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
     2941 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
     3001 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
     3061 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
     3121 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
     3181 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
     3241 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
     3301 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
     3361 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
     3421 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
     3481 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
     3541 ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccacgca
     3601 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
     3661 cgaacaatac tttaccctgc gaatcggtca tatagacatg atattcattg cgcactttgt
     3721 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca
     3781 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
     3841 ttgcggtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
     3901 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
     3961 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
     4021 aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
     4081 atcaacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca tcagttgctg
     4141 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
     4201 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
     4261 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
     4321 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
     4381 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
     4441 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccactga tatcaggcaa
     4501 accaacgtca agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
     4561 acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
     4621 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
     4681 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
     4741 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
     4801 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
     4861 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatggc
     4921 cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg ttttcgcccg
     4981 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
     5041 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
     5101 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
     5161 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
     5221 aacaaaatct caattctttt atttccgtca agccctaaaa catactctac taagaaaaaa
     5281 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
     5341 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
     5401 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
     5461 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
     5521 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
     5581 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
     5641 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
     5701 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
     5761 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
     5821 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
     5881 accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
     5941 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
     6001 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
     6061 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaat
     6121 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga tgccaaagcg
     6181 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc cgatccgtcg ttaacgctgc
     6241 aactcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacaacccag
     6301 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
     6361 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
     6421 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
     6481 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
     6541 tcaacggtgc cattgaccag ctgccgacgc cagctctctt cttcttcggt cagagaatcg
     6601 atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
     6661 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
     6721 ctgatgatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
     6781 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
     6841 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
     6901 taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc
     6961 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
     7021 atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc agtgaggaaa
     7081 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
     7141 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
     7201 aaaacgccag ccagcgcggc gaggcgcgtt cggctacttt gccgttgcct gcggtgattc
     7261 tggaaaaagt gcgtgaaggt gaggcacttg gtcccgtaat gtcgcgttat accggtattg
     7321 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
     7381 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat gcggtgtatt
     7441 aagcgcctga tgcgacgctg acgcgtctta ccatgctaca aaatcaatcg cttttcagca
     7501 acacctcttc cagccactgg cgcagctcga caggcgcggc tttcaggctg ttagatccac
     7561 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctgg ctcatttcgc
     7621 ctcgcagcag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
     7681 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
     7741 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
     7801 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
     7861 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
     7921 cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag cgtcataagc
     7981 cagcacgttc ttcacataac cacgcgtctc ggagaacgga atactctcga caaatgccac
     8041 tgcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgccctg gtccggcgtt
     8101 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcagata
     8161 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tactataacc
     8221 gggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
     8281 gctggcccct accggtgatt tcacttttgg attccaggcg ctctcctgac gggcaatcgc
     8341 catcgcatag ctttgcggga tctccttacc gctggtgtag cgtttgaaaa gatcgttgta
     8401 agccagcggg aatcgctctt ccagatgatc ccatagcttc ccggcgatcg ttgcctgaac
     8461 gctaagatcc caccattggt tgttgaaagc ataccgcgcc agttgagcct gctctgtttt
     8521 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
     8581 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
     8641 cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg
     8701 gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
     8761 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
     8821 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg
     8881 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
     8941 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
     9001 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
     9061 attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa
     9121 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
     9181 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
     9241 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
     9301 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
     9361 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
     9421 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
     9481 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
     9541 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
     9601 attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
     9661 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
     9721 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
     9781 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
     9841 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
     9901 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
     9961 tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    10021 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    10081 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    10141 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt
    10201 atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    10261 ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    10321 gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    10381 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    10441 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg
    10501 cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg
    10561 gcgttgtgcc gcctgcttct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    10621 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgaaggcacc
    10681 gttgtggcga ttactcacga ccgttacttc ctcgataacg ttgctggctg gatcctcgaa
    10741 cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa
    10801 gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    10861 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    10921 gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    10981 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    11041 tcctacggtg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc
    11101 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt
    11161 caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcatcggtt
    11221 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    11281 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    11341 aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt
    11401 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    11461 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    11521 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    11581 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    11641 gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag
    11701 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca
    11761 aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc
    11821 atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag
    11881 ccttagtatc cgcaaattcg tagcgggtca cccgtgctga ttcttttaaa ccttgtttaa
    11941 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc
    12001 ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac
    12061 gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    12121 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    12181 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    12241 acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca
    12301 gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac
    12361 tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa
    12421 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    12481 ggtggtattg aagatattag caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    12541 accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca
    12601 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    12661 atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    12721 cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt
    12781 gctccacaca tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat
    12841 gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt
    12901 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    12961 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    13021 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    13081 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    13141 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    13201 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    13261 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    13321 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    13381 cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg
    13441 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    13501 cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc
    13561 acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg
    13621 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    13681 ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    13741 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    13801 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    13861 tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca
    13921 tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    13981 gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag
    14041 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    14101 tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg
    14161 gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    14221 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac
    14281 agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc
    14341 gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc gcctagtacg
    14401 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    14461 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    14521 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    14581 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    14641 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg
    14701 agcaattact tctgattcag gctgcctgag aggatgcaga atacccccat caggtcagcg
    14761 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    14821 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    14881 gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg
    14941 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    15001 gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg
    15061 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag
    15121 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    15181 agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    15241 tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    15301 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    15361 gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt
    15421 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    15481 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    15541 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    15601 ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag gtcgcaccag
    15661 gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    15721 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    15781 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    15841 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacaac
    15901 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg ctgaacgttg
    15961 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    16021 taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    16081 cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcgg
    16141 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    16201 atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    16261 ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta gtgctgaccc
    16321 gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg ttaaccgcca
    16381 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    16441 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    16501 acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc
    16561 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    16621 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg
    16681 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    16741 ataaaaccat ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
    16801 aagcgtccgg acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag
    16861 gctcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg
    16921 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    16981 gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc
    17041 atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag
    17101 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    17161 cccgtcagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    17221 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    17281 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    17341 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    17401 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    17461 taaggtaact cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag
    17521 gagcgggaga ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt
    17581 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    17641 ctaaaataga tcgaaaagac ggaacggttt agctcaaaca tcaaaagcaa aatggcagaa
    17701 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    17761 tgaatagatt cgtgaataga tacttgaata gattttattt tctatccata tgaaaaataa
    17821 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtctc
    17881 ccactccggc ttcacacaac aatcgccttt cagcgaaaca caattactct ttatcgccca
    17941 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    18001 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt acagatggtc agggctttcg
    18061 cgccaaattc tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    18121 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    18181 acaggttacc aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca
    18241 ccatgtcaaa gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt
    18301 tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    18361 ctgcgccaca ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    18421 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    18481 ctttgtaagt accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    18541 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    18601 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa
    18661 catgttttca ccctctccct gtgggagagg gccggggtga gggcatcaag ccgcacccat
    18721 tatcagaaca tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc
    18781 tggccgatat ccgcgaaggt ttcacggtga cccagtgaac ccggttttac tttcgggccg
    18841 taaaccagta ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa
    18901 ccgtggtcag cagtgaggat caggatgtcg tcatcgcgca gcaaagacat cagctccggc
    18961 agacggcggt cgaacagctc cagacccgca gcataaccgg cgacgtcgcg acggtggccc
    19021 caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc
    19081 atctctttga tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg
    19141 ataccgcagt tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt
    19201 tcatcaacca gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg
    19261 ttaccggtac gctggaagtt accggctttg tcgccaataa acggacgagc gataacacga
    19321 ccgatgttgt agcagccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt
    19381 ttatccaagc caaaggtttc ttcgtggcag gcaatctgga acacggagtc agcggaggta
    19441 tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta
    19501 ccggaagagt ggcagttacc gagataacca ggcaggttag cgcgttcaac cagtttatcc
    19561 agcagctctt gcgggaagct gttttcgtga tcggagaagt atccccactc aaacagaacc
    19621 gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc
    19681 gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcctgccgg aatgaaaccg
    19741 gtagaacctt catgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggg
    19801 cctttacgac cgttatcagc ttcgcctttg gcacaggctt ctgcgatatg acccagggtg
    19861 tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc
    19921 agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg
    19981 atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt
    20041 gctttatcgg caagtttaat tgccgctttc accgctttcg ccgcgtcctg ccagctgttt
    20101 tcgtctttcg cgtggataac agccagcgga cgctgaccgt ctacctggtc gcccagacgc
    20161 gccatatcag taaagccgac gctgtaatcg atggtgtcag atgcctgacg gcgtccgccg
    20221 cccattgcaa ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg
    20281 gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca
    20341 acgaagtcgg tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta
    20401 ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg
    20461 ccggagatca gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga
    20521 tattcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag
    20581 gccagtacct gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc
    20641 acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg
    20701 ctacccactt tcacgtccat caccagcgca tccagacctt ccgccagttt cttcgccagg
    20761 atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcgcg ggtcgcgtag
    20821 aaacgtttat ctgccggagc cagtgagctg gtctggccga taatcgccac gccgacgtct
    20881 ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc
    20941 agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag
    21001 ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccgccg
    21061 gtggagtgtt tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt
    21121 cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag
    21181 aaaatggtca tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg
    21241 ttgataaaga aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata
    21301 atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc
    21361 ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc
    21421 agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg
    21481 cccagcgctt tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca
    21541 tctgcccagt cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc
    21601 gcagtacgca cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg
    21661 atcacttcca tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta
    21721 gaggttttga tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc
    21781 gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg
    21841 caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc
    21901 gcgcggtacg ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt
    21961 tctgccagcg cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt
    22021 cggatttccg gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga
    22081 tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt
    22141 actttctcgt cggtgtcgtc gtcattcaga gtggtcaggt ccatcaattt cagtgcacgc
    22201 aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac
    22261 cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa
    22321 ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac
    22381 tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac
    22441 ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca ctactcatga
    22501 aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca
    22561 gaagcgccat tttctggctc tggaagcaga aaacaatctg ccttatccgc agcttcctgc
    22621 cgaagcccgc cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc
    22681 gtacaaaccg cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg
    22741 gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta
    22801 ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt
    22861 gcaaccatat gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg
    22921 gcgttacctc gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga
    22981 ttcgcccatc acccgcgcga tcttacgtgc agacgcagat ttgaagcagg tttcaccgaa
    23041 cctgaccttt atctacgatc ctgaaatcac ccctgatgac ctgctgctga aagtggcgaa
    23101 gaacatctgt gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt
    23161 cacaaaaggg ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg
    23221 cagcacgctg gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga
    23281 cttctttacg cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg
    23341 gtgtgaattc ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg
    23401 gctgattaac cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt
    23461 taacttgctg tgtgagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa
    23521 tgaagtgggt tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa
    23581 atatggctgg caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg
    23641 caccacgccg ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca cccatctgca
    23701 aactgtcgcc ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga
    23761 cgaaaccatc aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg cctttaaagc
    23821 cggaatgcgt gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta
    23881 tatggtgcgt ttgtcggact tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac
    23941 ctggctgggc gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat
    24001 aagccatgaa cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg
    24061 ggccaggcag tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc
    24121 acaatccgtg gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc
    24181 atcaggcgtt gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt
    24241 gtgatacctg cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg
    24301 tggatgaggt gcttagccat atccgcaaag cggtgctgtt tattgaaggg ataacggtaa
    24361 gcggcggcga agccacgacc caactgccgt ttgtagtggc gctatttaca gccatcaaaa
    24421 acgatccgca actgcgccac ctcacctgcc tcgtggacag taacggtatg ttgagcgaaa
    24481 ccggctggga aaaattactc ccggtgtgcg acggcgcgat gctcgatctc aaagcgtggg
    24541 ggagcgaatg tcatcagcaa ttaaccggac gcgataatca gcagattaag cgcagcatct
    24601 gtttgctggc agagcgcggt aagctggcgg aactgcgttt gctggtaatt ccagaccagg
    24661 tggattattt gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc
    24721 cggttcgcct gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga
    24781 gcgccacgcc ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc
    24841 ggttgatatt tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacaa
    24901 acctgtaggc atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg
    24961 atgctgccta cgaagggtaa actacccacg aacgctaaac accgcatacg tattattaag
    25021 caacacttcg gcaatctcat cttctggttc cggacgtaac tcgcaaagca cgtcaaacac
    25081 ccgcgcagcc tgttccgggc ggttaggctt cccctgaaag ccgttgagcg gcatatccgg
    25141 cgcgtcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat cgcgggtttt
    25201 actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca gctgtacaaa
    25261 ccgctcggcc tgttgcaggc tgccggaaaa accgtgaacc acgccagtgc gcgataaatc
    25321 gtggcgttta agatgcatcg ccagtttgtc gtgtgtgcgc cgtgaatgca ggatcaccgg
    25381 cagatcgtag cgtttcgcca gtttcagctg ttcgtcgagt aaccactgtt gcctctcaaa
    25441 ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca ccttcgccgg
    25501 acgcctttcc agcgcctgct gtagctgatc aagagacaca tcgctatgtt tttccaacat
    25561 ccccggatgc aaccccaatg cggcataaag cggttgatat ttttccgcta acgcctgcac
    25621 gcgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc ccgcttgtgc
    25681 cgcgcgttgc aggctggcct cttcatcgcc actaaacggc gggaaatcaa aatggcagtg
    25741 ggtatcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg gtcgttcgcc
    25801 tgcggagcat cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc aggcggaata
    25861 acgatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc tttttcagtt
    25921 aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag tttatagtct
    25981 tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt cggtggataa
    26041 atttcgaata tccgcagctt gcccggtggt ttctcaataa attgctgaat gtcacgatag
    26101 ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc acccagccag
    26161 cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac agtgcggatg
    26221 acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc atcactgatc
    26281 ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaag gccaggtatc
    26341 gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa gtaattaggc
    26401 gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc aaacaaccgc
    26461 gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag ccagtcgaga
    26521 tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg ttttgtggta
    26581 tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc cgagaggttc
    26641 tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc gcgcataaac
    26701 tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca caccagagct
    26761 attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag cgttacaggt
    26821 attcgctgcc ccacctctgc cttcctgttt ttgttgtgaa agaatgacaa agtaacgcaa
    26881 tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga taaaattttc
    26941 ttaatggtct gaaatctagg gtcgttttcg gcccatgaac aaactcacca ggaacagaat
    27001 aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca gaccaccaaa
    27061 cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac gaaacataag
    27121 cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc actcaatgcg
    27181 atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac gaattacgac
    27241 gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
    27301 actttcagca cggtcgctct gtgcctggga atcgacggta ccggagagct gaaccacgcc
    27361 gtcggtggtt tcaactttca catgacggga agggacgata tcgtccgcca gcagtttggc
    27421 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcacagagc cttctttagc
    27481 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacccct tcaacgcctt tcgccacttt
    27541 cactgcctct tcggcctgag cctggctttc aacgaaaccg ctcagggtca cgactttttg
    27601 atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca gcgccgcctt
    27661 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
    27721 gactttttgc cctgcgcttt cattggtagt ctgtgcgttg ttttccgcgt aggcagagcc
    27781 ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct tcagtcttgt
    27841 catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctggca acacgaggtt
    27901 gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc catcacaaaa
    27961 gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat atcggtagaa
    28021 tgacaatcga cggctttaga tactgatatc tacgcattga acggtattta acgccgtcag
    28081 aaatgtcatt actttgttaa atatagatca caattttgaa accgctcggg atatcagcga
    28141 aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata aggaagggaa
    28201 attgacaggg cgaggcggtt gccgcgccct ggaggcaaga attaatgctc gcgggtctgg
    28261 tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttaaccat gctggtagcg
    28321 atgtaagcga gattatcgcc gccatcaagc gccagttggc tttcgttctt acgcttgaac
    28381 tcttcaaatt tcttcgcgtc cgcacattct acccagcggg cagtggcaac gttgactgac
    28441 tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ataccaccac atcaaactgc
    28501 agcacaccaa cagcaccaac gatcaaatcg ttgttggaga tcggacggaa cacctgcacc
    28561 gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt cagcggatct
    28621 ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt gaacttcatc
    28681 atctcaccct gggtaaaggt gtcgccgatc tgaatagtgc cgtggttgtg caggccgagg
    28741 atatcgcccg gatacgcttc ttcaacgtgc gaacggtcgc ccgccataaa ggtcagcgcg
    28801 tcggagataa ccacatcttt cgcagtacgc acctggcgca gcttcatgcc tttttcatat
    28861 ttaccggata ctacgcgcat aaacgccacg cggtcgcggt gtttcgggtc catgttggcc
    28921 tgaattttaa atacgaagcc ggtaaatttg tcttcgctcg cctctacggt acgggtatca
    28981 gtctgacgcg gcatcggcgc aggggcccac tccaccaggc catccagcat atgatcaacg
    29041 ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc aaggaacaat
    29101 tctttgtcga actcgttaga cgcgcctttc accagttcca gttcgtcacg tagctgctgt
    29161 gccagatctt caccaaccgc agcgtcgaga tccgggttat tcagcccttt aacaatacgg
    29221 acttcctgaa tcgtgtggcc tttaccgctc tgatagagat aggtttcgtc tttataaagg
    29281 tggtaaacgc ctttaaacag cttgccgcag ccaatcggcc aggtgatcgg tgcgcagccg
    29341 attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat atcacggtca
    29401 agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc catcagctta
    29461 cgggtacgat cttcaacacc ttttgcagcg tcgataacca tcaggcagca gtccaccgcc
    29521 gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta
    29581 accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat cccacgctgc
    29641 ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc ttttactgta
    29701 ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt agtcttaccg
    29761 gcatccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttg caaataagga
    29821 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
    29881 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
    29941 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
    30001 cgatggtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
    30061 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
    30121 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
    30181 agcaaatcga agtaatcacg caggcccgtg cgttccagac gcacttgctg caaggcgcta
    30241 aaaccgttgg tgatgatgcc gattttagcg ttgccacgaa tggcgttaag cagagaaacc
    30301 gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc gttaagcaag
    30361 cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat
    30421 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
    30481 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
    30541 aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
    30601 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
    30661 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
    30721 gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt
    30781 tttccagttc gtcgatcaga tgttccagca gcacccttcc caatccctga cgctgatagt
    30841 caggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
    30901 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
    30961 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
    31021 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
    31081 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
    31141 gcgccttcca gtgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
    31201 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
    31261 aatgcgcgca gaacatcgct cactaaagga tcggtcaggg cgggaagatc gtttgccacc
    31321 atcaccagac ggacgtgtgc cgggatcgca atggcaatct ccccctgcaa cgcgccaggg
    31381 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
    31441 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
    31501 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
    31561 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
    31621 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
    31681 cccagcaatt ccaccactgg caggtattaa gccgccagat gggggataac gcccgtttca
    31741 gtctggtcgc cacggcggat gacgtcgcag attgcgatac gctgatttac tactggccga
    31801 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccggtgggga
    31861 cggatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgccgag cagatgctgg
    31921 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc
    31981 gtctggaaaa acagccggta tttgatgccg ataaattctg gggcgaatac agcgtcgatg
    32041 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
    32101 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
    32161 gcgcgggggt gctttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
    32221 gcgatgtctc tgcgccagcg gtagaagcca gccgcgcaac acttgcggcc aactgtgttg
    32281 aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggtcgtttt gatatgatca
    32341 tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
    32401 ttcgcggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
    32461 tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtg attgcgcaaa
    32521 ccgggcgttt caaggtgtat cgcgccatta tgacccgcca ggcgaagaaa ggttaattat
    32581 ctcgccgaat accgtgtcgg atgcggcgtg aacgccttat ccgacctaca aaacctgcac
    32641 gttagccctt cgtaggccag ataagacgcg ccagcgtcgc atctggcatc tgcccccgca
    32701 tgcctaccaa acctgcacgc cagtttcctg atggacattt ttccagcaat tacacctccg
    32761 gcgataatta actattgacg aaaagctgaa aaccactaga atgcgcctcc gtggtagcaa
    32821 ttctttttaa gaattgatgg tatgcgaagg tggcggaatt ggtagacgcg ctagcttcag
    32881 gtgttagtgt ccttacggac g
//
LOCUS       DAZFAH010000032        32404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFAH010000032 DAZFAH010000000
VERSION     DAZFAH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.032"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..1836)
                     /gene="ycaO"
                     /locus_tag="ACHJWB_001410"
     CDS             complement(76..1836)
                     /gene="ycaO"
                     /locus_tag="ACHJWB_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4313065.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1966..2658
                     /locus_tag="ACHJWB_001411"
     CDS             1966..2658
                     /locus_tag="ACHJWB_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4313066.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2857..3945
                     /gene="serC"
                     /locus_tag="ACHJWB_001412"
     CDS             2857..3945
                     /gene="serC"
                     /locus_tag="ACHJWB_001412"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4313067.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCMPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4016..5299
                     /gene="aroA"
                     /locus_tag="ACHJWB_001413"
     CDS             4016..5299
                     /gene="aroA"
                     /locus_tag="ACHJWB_001413"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4313068.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5468..6232
                     /gene="ycaL"
                     /locus_tag="ACHJWB_001414"
     CDS             5468..6232
                     /gene="ycaL"
                     /locus_tag="ACHJWB_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4313069.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6405..7088
                     /gene="cmk"
                     /locus_tag="ACHJWB_001415"
     CDS             6405..7088
                     /gene="cmk"
                     /locus_tag="ACHJWB_001415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4313070.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7199..8872
                     /gene="rpsA"
                     /locus_tag="ACHJWB_001416"
     CDS             7199..8872
                     /gene="rpsA"
                     /locus_tag="ACHJWB_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4313071.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9032..9316
                     /gene="ihfB"
                     /locus_tag="ACHJWB_001417"
     CDS             9032..9316
                     /gene="ihfB"
                     /locus_tag="ACHJWB_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4313072.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9523..11786
                     /locus_tag="ACHJWB_001418"
                     /pseudo
     CDS             9523..11786
                     /locus_tag="ACHJWB_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            11823..13571
                     /gene="msbA"
                     /locus_tag="ACHJWB_001419"
     CDS             11823..13571
                     /gene="msbA"
                     /locus_tag="ACHJWB_001419"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4313073.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13568..14554
                     /gene="lpxK"
                     /locus_tag="ACHJWB_001420"
     CDS             13568..14554
                     /gene="lpxK"
                     /locus_tag="ACHJWB_001420"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4313074.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADFSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14591..15823
                     /gene="ycaQ"
                     /locus_tag="ACHJWB_001421"
     CDS             14591..15823
                     /gene="ycaQ"
                     /locus_tag="ACHJWB_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO4313075.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPIRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15875..16057
                     /gene="ycaR"
                     /locus_tag="ACHJWB_001422"
     CDS             15875..16057
                     /gene="ycaR"
                     /locus_tag="ACHJWB_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4313076.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16054..16800
                     /gene="kdsB"
                     /locus_tag="ACHJWB_001423"
     CDS             16054..16800
                     /gene="kdsB"
                     /locus_tag="ACHJWB_001423"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4313077.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16954..17847
                     /locus_tag="ACHJWB_001424"
     CDS             16954..17847
                     /locus_tag="ACHJWB_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4313078.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17824..18603)
                     /gene="elyC"
                     /locus_tag="ACHJWB_001425"
     CDS             complement(17824..18603)
                     /gene="elyC"
                     /locus_tag="ACHJWB_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4313079.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18739..19524
                     /gene="cmoM"
                     /locus_tag="ACHJWB_001426"
     CDS             18739..19524
                     /gene="cmoM"
                     /locus_tag="ACHJWB_001426"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4313080.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19521..20843
                     /gene="mukF"
                     /locus_tag="ACHJWB_001427"
     CDS             19521..20843
                     /gene="mukF"
                     /locus_tag="ACHJWB_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4313081.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20824..21528
                     /gene="mukE"
                     /locus_tag="ACHJWB_001428"
     CDS             20824..21528
                     /gene="mukE"
                     /locus_tag="ACHJWB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4313082.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21528..25988
                     /gene="mukB"
                     /locus_tag="ACHJWB_001429"
     CDS             21528..25988
                     /gene="mukB"
                     /locus_tag="ACHJWB_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4313083.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIAELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLERAREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLVVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            26249..28096
                     /gene="ldtD"
                     /locus_tag="ACHJWB_001430"
     CDS             26249..28096
                     /gene="ldtD"
                     /locus_tag="ACHJWB_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4313084.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28277..28825
                     /locus_tag="ACHJWB_001431"
     CDS             28277..28825
                     /locus_tag="ACHJWB_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4313085.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28852..29499
                     /gene="gloC"
                     /locus_tag="ACHJWB_001432"
     CDS             28852..29499
                     /gene="gloC"
                     /locus_tag="ACHJWB_001432"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4313086.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLTALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVLGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29721..30911)
                     /gene="aspC"
                     /locus_tag="ACHJWB_001433"
     CDS             complement(29721..30911)
                     /gene="aspC"
                     /locus_tag="ACHJWB_001433"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4313087.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31096..32184)
                     /gene="ompF"
                     /locus_tag="ACHJWB_001434"
     CDS             complement(31096..32184)
                     /gene="ompF"
                     /locus_tag="ACHJWB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4313088.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7897 a         7868 c         8711 g         7928 t
ORIGIN      
        1 gcggggcatt aaaaaaacgc cccgtaatat aactcagact aatcattaag cctacattgc
       61 gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta
      121 ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag
      181 gccgtaaaac gccgcttcgc cgctcattgc cgcactggcg gcttctacgg catctgcgcc
      241 gtacatgcga acaaacgcat tcagatattg cagcggctgg cgatcttctt cctgtgccag
      301 taataacaac gtttgcaggc agcgataata gttggcgcgt tccggactaa atactgatga
      361 gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc caccagccag
      421 cgccagcatg gcttttaatt caccgatacg cagggtgtac caaccgttat ccgacccggt
      481 cgccagaccc aacagctcac gcacgcgggt aaagtcatca aaaccttctt catccagttg
      541 ctcgatgagg ttcaggtaat cttctttttc ccactcgctg cctggtagcg aaagaatcgt
      601 ttcacgtaaa tggctgccca tactgttatt cgcgagccac agatcttcag ccggataaat
      661 atcggacatg ccaggcacga taatgcggca agcgtagaca cccagatgct cgtaatcggc
      721 aatataaact tctttatctt cctttttgaa gatagccatc agcgtggcga actcttcttc
      781 cgtggtgccg gagaaattcc agtccacaaa cggataatcg gcatcctgct tgaacaggtc
      841 ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat gttcagcgac
      901 ttcttcatca tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc cacgaccttg
      961 cagcagctcg gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga
     1021 ggcaaagcag gtaccgttag caggattgaa cagtaccacg caaatcaccg gatactggcc
     1081 gccaagcgaa ccgtcataag cgaagattgg gaaaccctcc gcttccagcg tttcgatggc
     1141 ttcaactact gctgggtaac gcgccagcac gtctgccggg atctctggca ggctgatgct
     1201 ttcagcaata atgcggtttt tcacgtagcg ttcgaaaact tcagacaatc cctgaacgcg
     1261 tgcttcgttg cgggtattac ctgcggacat accgttggag acgtacaggt taccaatgat
     1321 attcatcgga atataaacgg tctgattgtc ggactgacgc gtaaacggca ggccgcaaat
     1381 accacgatct tcgttaccgg attgtaggtc aatcagcatg ctgccggtca gttcattctc
     1441 cggatcataa aacgcgcgca gacggtcatc gagtagtcct tctggcacat cgtcattttc
     1501 ggtcagtggg aaccattttt cgttgggata atgcacgaac ggaccgttgg cgatggtttc
     1561 gcccagccag aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat attcaccaag
     1621 tgcagaagcc agcgccgctt ttttggttgc gcctttaccg ttggtaaaac acagtgcgca
     1681 ctctttgtcg cgaatatgta cagaccagac gttaggcaca ggattcagcc aggaggcctc
     1741 ttcaatctga aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg aatcttccag
     1801 agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag tcgtacggaa
     1861 agcgcgcaat aatacgggtt ttatctcaaa ggcgctatca ccgccgccat gccgatgaac
     1921 tgttgactat gcttttagcg gataaaccac gataagagca taaaaatgaa ggcgttcgat
     1981 ctccaccgta tggcatttga taaagtgcct tttgatttcc ttggcgaagt tgcactacgt
     2041 agtctttata cctttgtact ggtttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     2101 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     2161 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     2221 ttgttatacc gcctggtaat gtggttgatg gcgcacagtg aaaaactgga agatttactg
     2281 gaaggtaagc cggttgttat tattgaagat ggcgagctgg cctggtcgaa actcaataac
     2341 tccaacatga cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg
     2401 gggcaggtac gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa
     2461 gatgacaagg tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa
     2521 gtggtgccgg agtcggcgga ctatgcctgt attcgttgta gtgaaatcat tcatatgaaa
     2581 gcgggggaaa aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg
     2641 gcaaaacggg tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct
     2701 tttgtgtgat gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca
     2761 ctgaatgata aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga
     2821 ccgcgggtta atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt
     2881 tctggtccgg caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac
     2941 tggaacggtc ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag
     3001 gttgcagagg aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag
     3061 gtattattct gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc
     3121 ggtgataaaa ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa
     3181 gaagcgaaaa aatactgcat gcctaatgtc tttgacgcca aagtgactgt tgatggtctg
     3241 cgcgcggtta agccaatgcg tgaatggcaa ctctctgata atgctgctta tatgcattac
     3301 tgcccgaatg aaaccatcga cggtatcgcc atcgacgaaa cgccagactt cggcaaagat
     3361 gtggtggtcg ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt
     3421 tatggcgtga tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc
     3481 atcgttcgtg aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat
     3541 tccatcctca acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta
     3601 tctggtctgg tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc
     3661 aatcagcaaa aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat
     3721 gacgtggcga aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg
     3781 cttgacaaat tgttccttga agagtctttt gctgctggcc ttcatgcact gaaaggtcac
     3841 cgtgtggtcg gcggaatgcg cgcttctatt tataacgcca tgccgctgga aggcgttaaa
     3901 gcgctgacag acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct
     3961 taatccccac agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga
     4021 atccctgacg ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa
     4081 gagcgtttct aaccgcgctt tattgctggc ggcattagca cacggcaaaa cagtattaac
     4141 caatctgctg gatagcgatg acgtgcgcca tatgctgaat gcattaacag cgttaggggt
     4201 aagctatacg ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt
     4261 acacgcagaa ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct
     4321 ggcggcagct ctttgtctgg gtagcaatga tattgtgctg accggtgagc cgcgtatgaa
     4381 agaacgcccg attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta
     4441 cctggaacaa gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt
     4501 tgacgttgat ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct
     4561 tgcgccggaa gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga
     4621 catcacactc aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca
     4681 atttgtcgta aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga
     4741 tgcatcttcg gcttcttact ttctggcagc agcagcaatc aaaggcggca ctgtaaaagt
     4801 gaccggtatt ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa
     4861 aatgggcgcg accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa
     4921 cgctattgat atggatatga accatattcc cgatgcggcg atgaccattg ccacggcggc
     4981 gttatttgca aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagaaac
     5041 cgatcgcctg tttgcgatgg caacagaact gcgtaaagtc ggtgcggaag tagaagaggg
     5101 gcacgattac attcgtatca ctccaccgga aaaactgaac tttgccgaga tcgcgacata
     5161 caatgatcac cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac
     5221 gattcttgat cccaaatgta cggccaaaac atttccggat tatttcgagc agttggcgcg
     5281 gattagccag gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa
     5341 aacgtcggca cattgtcggc gttttttttc ggaccttgtg agtcattttg attaatggta
     5401 gcgtcgcttg tcaatgtaag ttgttgatac ataatattta tatatgatta atcaacggat
     5461 gattcacatg aagaatacta aattactgct ggcgattgcg acctctgcag cattactgac
     5521 agggtgtcaa aatacccacg gtattgatac caatatggct atcagctccg gtttaaatgc
     5581 ctataaagca gcaacattaa gcgatgccga tgcaaaagcg attgccaatc agggctgtgc
     5641 cgaaatggac agcggcaatc aagtcgcaag taaatccagc aagtacggta aacgtctggc
     5701 aaaaatcgcc aaagcattgg gtaacaatat taatggcacg ccggtcaact ataaggttta
     5761 tatgaccagc gacgtcaacg catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg
     5821 cctgatggac atgatgaatg acaacgaaat tgaaggtgtt ctgggccatg aactgggcca
     5881 cgtcgcgttg ggtcactcgc tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg
     5941 cgatgccatt tcagctacca gcggagtggc ttcccagctt tcccgctcac aattgggtga
     6001 tatcgcagaa ggcgctatta atgctaaata ctcccgtgat aaagagtccg aagcagatga
     6061 tttctccttt gatctgttga agaaacgtgg catcagcacc caggggctgg ttggcagctt
     6121 tgaaaaactg gctagtctgg atggcggtcg cacccagtcc atgtttgact ctcacccacc
     6181 atcaacagag cgtgcgcaac acatccgtga tcgtatcgcc tctggtaagt aaatcattgt
     6241 catctttcgg gctggtcttc tgccagcccg ctataattgc gcaataaatc cccatctgaa
     6301 tacagacaaa actggttttt gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat
     6361 gcgcgcggtt atgttaacgg tacgcctgtt ttaaggagat aaagatgacg gcaattgccc
     6421 cggttattac cattgatggc ccaagcggtg cagggaaagg caccttgtgt aaggctatgg
     6481 cggaagcatt gcaatggcat ctgctggatt cgggtgcaat ttatcgcgta ctggcattgg
     6541 cggcattaca tcaccatgtt gatgttgcgt cagaagatgc gctggtaccg ctggcatccc
     6601 atctggatgt acgttttgtg tcaaccaatg gcaatctgga agtgatcctc gaaggggaag
     6661 atgtcagcgg cgaaattcgt actcaggaag tggcgaatgc agcttcacaa gtcgcggcat
     6721 tcccacgcgt tcgtgaagca ttattgcgtc gccaacgcgc gtttcgcgaa ttaccaggcc
     6781 tgattgccga tggccgcgac atgggaacgg tggtattccc tgatgcgcca gtgaaaattt
     6841 tccttgacgc ctcctcggaa gaacgtgcgc atcgccgcat gctacagttg caggagaagg
     6901 gctttagtgt taactttgag cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc
     6961 gtaaccgagc ggtagcgcca ctggttccgg cagccgatgc tttagtgttg gattccacca
     7021 ccttaagcat tgagcaagtg attgaaaaag cgctacaata cgcgcgccag aaattggctc
     7081 tcgcataagc gaccgaattt gcagtacccc cgttgcaatg gaatgacagc gggtatgtta
     7141 aacaacccca tccggcatgg agccaggtgg acgttaaata taaacctgaa gattaaacat
     7201 gactgaatct tttgctcaac tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg
     7261 ttctatcgtt cgtggcgttg ttgttgctat cgacaaagac gtagtactgg ttgacgctgg
     7321 tctgaaatct gagtccgcca tcccggctga gcagttcaaa aacgcccagg gcgagctgga
     7381 aatccaggta ggtgacgaag ttgacgttgc tctggacgca gtagaagacg gcttcggtga
     7441 aactctgctg tcccgtgaga aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc
     7501 ttacgaagat gctgaaactg ttaccggtgt tatcaacggc aaagttaagg gcggcttcac
     7561 tgttgagctg aacggtattc gtgcgttcct gccaggttct ctggtagacg ttcgtccggt
     7621 gcgtgacact ctgcacctgg aaggcaaaga gcttgaattt aaagtaatca agctggatca
     7681 gaagcgcaac aacgttgttg tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga
     7741 gcgcgatcag ctgctggaaa acctgcagga aggcatggaa gttaaaggta tcgttaagaa
     7801 cctcactgac tacggtgcat tcgttgatct gggcggcgtt gacggcctgc tgcacatcac
     7861 tgacatggcc tggaaacgcg ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat
     7921 cactgttaaa gtgctgaagt tcgaccgcga acgtacccgt gtatccctgg gcctgaaaca
     7981 gttgggcgaa gatccgtggg tagctatcgc taaacgttat ccggaaggta ccaaactgac
     8041 tggtcgcgtg accaacctga ccgactacgg ctgcttcgtt gaaatcgaag aaggcgttga
     8101 aggcctggta cacgtttccg aaatggattg gaccaacaaa aacatccacc cgtccaaagt
     8161 tgttaacgtt ggcgatgtag tggaagttat ggttctggat atcgacgaag aacgtcgtcg
     8221 tatctccctg ggtctgaaac agtgcaaagc taacccgtgg cagcagttcg cggaaaccca
     8281 caacaagggc gaccgtgttg aaggtaaaat caagtctatc actgacttcg gtatcttcat
     8341 cggcctggac ggcggcatcg acggcctggt tcacctgtct gacatctcct ggaacgttgc
     8401 aggcgaagaa gcagttcgtg aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca
     8461 ggttgacgca gaacgtgaac gtatctccct gggcgttaaa cagctcgcag aagatccgtt
     8521 caacaactgg gttgctctga acaagaaagg cgctatcgta accggtaaag taactgcagt
     8581 tgacgctaaa ggcgcaaccg tagaactggc tgacggcgtt gaaggttacc tgcgtgcttc
     8641 tgaagcatcc cgtgaccgcg ttgaagacgc taccctggtt ctgagcgttg gcgacgaagt
     8701 tgaagctaaa ttcaccggcg ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc
     8761 gaaagacgaa gctgacgaga aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa
     8821 cttctccaac aacgcaatgg ctgaagcttt caaagcagct aaaggcgagt aattctctga
     8881 ctcttcggga tttttatttc gaagtttgtt gagtttactt gacagattgc aggtttcgtc
     8941 ctgtaatcaa gcactaaggg cggctacggc cgcccttaat caatgcagca acagcagccg
     9001 cttaatttgc ctttaaggaa ccggaggaat catgaccaag tcagaattga tagaaagact
     9061 tgccacccag caatcgcaca ttcccgccaa gacggttgaa gatgcagtaa aagagatgct
     9121 ggagcatatg gcctcgactc ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag
     9181 tttctctttg cactaccgcg caccacgtac cggacgtaat ccgaagactg gcgataaagt
     9241 agaactggaa ggaaaatacg ttcctcactt taaacctggt aaagaactgc gcgatcgcgc
     9301 caatatttac ggttaagttt ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc
     9361 ttttttcatt tctctaatca ggaactggaa gctgcctcgc agagttttga gcagttttca
     9421 ccctttcgtt aaattcttct gaatgtgcct cggggaacgc aaaattccca ccagacaacc
     9481 gctcaacaaa gttgcacact ttccataaac agggaggggt gcatgaaaat aacgacagtc
     9541 ggcgtatgca taatttgcgg aatttttccg ttgctgattt tgccccaatt gcctgggaca
     9601 ttaacccttg cgtttctgac tctcttcgcc tgcgtactgg catttatccc tgttaaaacc
     9661 gtccgttata tcgcgctgac gttgctgttt ttcgtttggg gcatattatc agcaaagcaa
     9721 attttgtggg caggagaaac cttaactggc gcgacgcagg atgcaattgt tgagatcact
     9781 gcaactgacg gcatgaccac tcattacggt caaattactc atctacaagg tcgacgtata
     9841 ttccctgcgc caggccttgt gctgtatggc gaatatcttc cgcaagcggt ttgtgctgga
     9901 caacaatggt caatgaaact caaagttcgt gcggttcatg gccaacttaa tgatggcggc
     9961 tttgatagcc agcgttatgc cattgcccag catcaaccgc tcaccggccg ctttctgcag
    10021 gcaagtgtta ttgaaccgaa ttgtagcctg cgtgcacagt atctggcgtc accacaaaca
    10081 acgctgcaac cctatccgtg gaatgcggtt attcttggtt taggtatggg ggacggttat
    10141 ccgtccccaa agaaatcaaa aatatcatgc gcgatactgg aacggcgcat ttaatggcga
    10201 tatcgggatt gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac
    10261 aaatttttct gcctgggcgc tggatccact ggcaaatacc attaattggt gggatctgct
    10321 gtgctgcttt ttatgcctgg ttgacgggaa tgcaacctcc tgcattgcgt accgtggtgg
    10381 cgcttgctac gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat
    10441 ggatatgttg tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt
    10501 tatggctctc tgccgctgcg gtcgcggcac tgattttttg gtatcagtgg tttccctgtc
    10561 ctgagtggca actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg
    10621 gtatcacact tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct
    10681 cgtttattgc aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc
    10741 tcgccgcgat ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc
    10801 ttgccgaccg gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga
    10861 tcaacattgc tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat
    10921 ggcgattaaa cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt
    10981 gctggccgct atggcaaaaa cctcgacctg acgagtggca ggtgtacatg cttgatgtcg
    11041 ggcaagggct ggcaatggtg atagccagaa acggcaaagc gattctctat gacacaggac
    11101 tggcctggcc cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata
    11161 atcttgaacc ggaaggcgtt attctgagtc atgaacatct ggatcaccgg ggagggctgg
    11221 actcaatatt gcacacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc
    11281 atcagccctg tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgtgcact
    11341 ggcctttaca agctagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg
    11401 acgggacgaa tagcattctt ctaaccggtg atattgaagt ccccgctgaa caaaagatgc
    11461 taagccgtta ctggcagcaa gtgcagacaa cattgcttca ggtacctcac catggcagta
    11521 atacctcatc atcgttgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg
    11581 catcgcgcta taacgcatgg cgattgccct ctaataaagt taagcatcgc tatcaacagc
    11641 aaggatatca atggcttgat actccacatc agggtcaagt gacggtcaat ttttcagcgc
    11701 aaggctggcg gattagcagc ctcagagagc aaattttacc tcgttggtat catcagtggt
    11761 ttggcgtgcc agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt
    11821 gaatgcataa cgacaaagat ctctctacgt ggcagacgtt ccgccgactg tggccaacca
    11881 ttgcgccttt taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca
    11941 gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag
    12001 atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta
    12061 tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca
    12121 tgcgtcgccg cctgtttggt cacatgatgg gaatgccggt ttcatttttt gacaaacagt
    12181 caacggggac gctgttgtca cgtatcacct acgattccga acaggttgct tcttcttctt
    12241 ccggcgcact gataacggtt gtgcgtgaag gtgcgtcaat catcggcctg ttcatcatga
    12301 tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga
    12361 ttgcgattcg cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca
    12421 tggggcaggt gaccaccagc gccgaacaaa tgctgaaggg ccacaaagaa gtattgattt
    12481 tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc
    12541 aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct
    12601 ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga
    12661 ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat
    12721 cactgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta
    12781 ccattctgga cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg
    12841 gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc
    12901 gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt
    12961 caggtaaatc aaccatcgcc agcctgatca ctcgttttta cgatattgat gaaggcgaaa
    13021 tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg
    13081 ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg
    13141 cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca
    13201 tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc
    13261 tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgctg cgtgatagcc
    13321 cgattctgat tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc
    13381 aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt
    13441 ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg
    13501 gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt
    13561 ttggccaatg atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc
    13621 actctcttgg ttgtatggcc tggtaagtgg cgcgatccgt ctttgctata aactaaaact
    13681 gaagcgcgcc tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg
    13741 caacggaaaa accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg
    13801 cgtgggggtc gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc
    13861 ggcagatacc acaacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga
    13921 tgcgcctgtt gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca
    13981 ccctgatgtg cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga
    14041 tgtggaaatt gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc
    14101 ggggccaatg cgtgagcgag cggggcgctt aaagtcggtt gatgcggtaa tcgtcaacgg
    14161 cggtgtccct cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt
    14221 acgtaccggt acgcgttgtg acgttgctca gcttgaacat gtagtggcga tggcggggat
    14281 tgggcatccg ccgcgctttt ttgccacgct gaagatgtgt ggcgtacaac cggaaaaatg
    14341 tgtaccgctg gccgatcatc agtctttgaa ccatgcggat ttcagtgcgt tggtaagcgc
    14401 cgggcaaacg ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga
    14461 aaattggtgg tatttgcctg tagacgcaca gctttcaggt gatgaaccag cgaaactgct
    14521 tacgcaacta acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat
    14581 ggagtcatga atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc
    14641 cgcacaaggc ctgttaaaca aaccccgccg tcaagcgtcg ttggaggata ttccggcaac
    14701 gatctcccgc atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagtccata
    14761 tctggtgctt ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc
    14821 gcgtggcgaa ttaatggaat actgggcgca tgaagcctgc tttatgccgc gtagcgactt
    14881 tcgtcttatt cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc
    14941 ctggatgcag gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg
    15001 gccgatacgt tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg
    15061 gaagccgcat aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga
    15121 acggcgcaac ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga
    15181 tgagcgcgat ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg
    15241 tagcctggga atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc
    15301 actggcggcg tggcgcgaag cgagggctga acagcagcaa atcattgctg tgcatgttga
    15361 aaaattgggc aatctttggc tgcatgctga tttgctgccg ctactcgagc gtgcgctggc
    15421 cggaaagctc actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga
    15481 tcgcaaacgc gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc
    15541 gccgaaacgc cagtatggct attttgttct gccattatta catcgtgggc aattagttgg
    15601 gcgaatggat gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt
    15661 acaggaaggc attaaaccaa cgacaatgct gcaaaaaggg ttacgtcagg cgattactga
    15721 tttcgcgagc tggcagcagg caacgcgggt gacattagga cgctgcccgc aaggtctctt
    15781 tactgattgc cgcactggct gggaaataga ccccgtcgca taaatgatta tgataagatt
    15841 tatgcattca taacccgatc catctggagg aactatggat catcgtctgc ttgaaatcat
    15901 tgcctgcccg gtttgcaacg gaaaactttg gtataaccaa gaaaaacaag aacttatttg
    15961 caaactggac aacctggctt tcccgctgcg tgatggcatt ccagtgttgc tggaaacgga
    16021 agcccgcgtg ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg
    16081 cgctacgcgt caacgcgtct gcccggtaaa ccattggttg atattaatgg caaacccatg
    16141 attgttcatg ttcttgaacg tgctcgcgaa tcaggtgccg atcgcatcat cgtggcaacc
    16201 gatcatgagg atgtcgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc
    16261 gccgatcacc agtcagggac agaacgcctg gcggaagttg tcgaaaaatg cgcattcagc
    16321 gacgacacgg tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt
    16381 cgtcaggttg ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca
    16441 atccacaatg cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa
    16501 gggtatgcac tgtacttctc tcgcgccacc attccttggg atcgtgatcg ttttgcagaa
    16561 ggccttgaaa ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca
    16621 ggctttatcc gtcgttacgt caactggcag ccaagtccgt tagaacacat cgaaatgtta
    16681 gagcagcttc gtgttctgtg gtacggcgaa aaaatccatg ttgctgttgc tcaggaagtt
    16741 cctggcacag gtgtggatac ccctgaagat cttgagcgcg ttcgcgctga aatgcgctaa
    16801 tttcacttca cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct
    16861 caatttttct tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt
    16921 atgaaagcag tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc
    16981 catttactgg gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg
    17041 ttgtgggctt tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt
    17101 acgcccggaa aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact
    17161 gtccgtatgc cgactattta tggtgtgatg acgcatgaag aacaccccgg ccccgatgtc
    17221 ctgctactgg agcggatgcg tggtgtttcg gtggaggcac cagcccgaac accagaacgc
    17281 tgggaacaac tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt
    17341 cgcggttgcg tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg
    17401 caacatgttg aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg
    17461 caggataagc ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt
    17521 aacgacaatt gtgtgctgat tcacggtaac ttctgtttac gcagcatgtt gaaagattcg
    17581 cgcagcgatc agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa
    17641 tacgaactgt tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg
    17701 caacgcgcgc cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat
    17761 gaagttgcgc aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca
    17821 aaatcactct tgccgtggct cgcctgacga accttttagc cactgccaga tacggccaag
    17881 cgtttcatag ccaacacgat cgctatgcat caaccagacc ggagaaggga ttgcccgttc
    17941 ccacgggtta agcggcgagt cgatggccaa ctgattcgcc ggggctggta gcggatttaa
    18001 cccttcctgc tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag
    18061 gaaaggggcg tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg
    18121 caaatccagg gtgataattt gctcgtgcgg cacgcccagc gattgcgcaa ctctggcacc
    18181 tacttccgct gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga
    18241 tcccggattt tcgcgccata agcgaatacc ttcattcagg cgtggcagac tgttattgat
    18301 taaattagag ctcggtgccc actgtgggtt ccaggtataa ccacctccga gcaccacgat
    18361 atagtccact ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag
    18421 gcggtcggct accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa
    18481 aatcttcccg gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat
    18541 taacaacatc agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag
    18601 catcctttct ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag
    18661 acgggttcat tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc
    18721 caaccggtgg agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc
    18781 cgtaacattt acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc
    18841 gatcacgtgc tggcggaaat gggcccgcaa aaactgcgtg tgctggatgc tggcggtggt
    18901 gaagggcaga ccgcaatcaa aatggccgag cgtgggcatc aggtcatttt atgcgatctt
    18961 tctgcgcaga tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac
    19021 atgcaattta tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat
    19081 ctgatattgt tccatgcggt gctcgagtgg gtggccgatc cccgcagtgt attgcagacc
    19141 ctgtggtcag tgttgcgtcc aggcggagtg ttgtcgttaa tgttctacaa tgcgcatggt
    19201 ttgttgatgc ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa
    19261 aagaaaaaac ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg
    19321 ctggaagaag ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat
    19381 ctgcgcgaga agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt
    19441 tattgccgtc aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa
    19501 ccgcagagca aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct
    19561 gggccagaaa aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg
    19621 cggttgccac gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg
    19681 atgcattccg ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg
    19741 ccaacaacgc gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc
    19801 aggcggaagg gaacgcaatt taccgtctga cgccgctcgg catcggcatc actgactatt
    19861 acattcgtca gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg
    19921 gtgagctcaa acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc
    19981 gtaatgttta tgctccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga
    20041 cgcaacgtct gatggatgaa cagcagcagc aggtgaagga tgatatcgcc cagttgctga
    20101 acaaagactg gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa
    20161 cgctgcgtga attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt
    20221 tgcgcattca ggatgcgacg atgacccatg acgatctgca tttcgtcgat cgtctggtgt
    20281 tcgatctgca gagcaaactc gatcgtatta tcagttgggg ccagcaatcc atcgacttgt
    20341 ggattggcta cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa
    20401 accgcgtctt tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg
    20461 cgctaactta tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc
    20521 gcgatgaaga agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga
    20581 tccgcgaaca gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag
    20641 tgccgctgga tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc
    20701 actttgacgt tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag
    20761 atttcaccgg actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg
    20821 cgcatgtcat cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg
    20881 aatccgttat ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac
    20941 gaactggata atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg
    21001 cgttataacg ttgagcttat tcgcgcaccg gaagggttct tctatttacg cccacgttcc
    21061 accactctta tcccgcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc
    21121 tgttatctct atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa
    21181 ctgtacgacg aactgctcac cctggccgat gaagcaaaac tgctgaaact ggtgaacaac
    21241 cgttcaaccg gttcagacgt tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc
    21301 aaccgtctgc gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagtttcgc
    21361 attaccgaat cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa
    21421 gcacagcgtc gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc
    21481 aacgatgaaa ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg
    21541 gtaaatttcg ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc
    21601 ttgacgagct ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg
    21661 cggcgttcgt tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg
    21721 aagccggggc caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg
    21781 tctgttattc gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc
    21841 gtctgcaaca ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg
    21901 gactgccgat gtcggtgcag ccgacacagc tggtgaccga aaccctgaac gaacgccagg
    21961 cgcgcgtgct gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt
    22021 ttaaacagtt caactccatt accgattacc actcgctgat gttcgatctg ggcatcatcg
    22081 cgcgtcgtct gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc
    22141 tgtatggcgg gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa
    22201 acagcggcgt gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga
    22261 cgctggaagc gattcgtgtc acccagtcgg accgtgacct gtttaagcat ctgatcagcg
    22321 aagctaccaa ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc
    22381 tcgacaaagc cctggagttt cgtcgcgagt tacatacttc gcgtcagcaa ctggcggctg
    22441 agcagtacaa acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg
    22501 atctggaagc ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc
    22561 gtcagcagga gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg
    22621 aagagcaaaa cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgcg
    22681 cggaagctgc cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg
    22741 cgctggacgt ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc
    22801 gcgccaaaga actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg
    22861 aaaccttcca ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa
    22921 tgagcatggc gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa
    22981 tcaacggccc gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag
    23041 gggtcgatca gcgtcacctg gcagagcagg ttcaaccgtt gcggatgcgc ttaagtgaac
    23101 tggaacagcg tctgcgcgag cagcaagaag ccgaacgtct gctggcagat ttctgcaaac
    23161 gtcagggcaa gaattttgat atcgccgaac tggaagccct gcatcaggaa ctggaagcac
    23221 gcattgcctc tctttccgat agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc
    23281 aggagcagga acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc
    23341 tggcagcgca aaacagtctc aaccagttga gcgaacagtg cggcgaagag tttacctcca
    23401 gccaggacgt caccgaatat ctgcaacagt tgctggagcg tgcgcgagag gcgattgttg
    23461 aacgcgatga agtgggcgcg cgcaaaaacg ccgtcgatga agagatcgaa cgtttaagcc
    23521 agcctggcgg ctctgaagat cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc
    23581 tgctgtcaga aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt
    23641 atggcccgtc acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg
    23701 aaggcttgac cgattgcccg gaagatctct atctgatcga aggagatccg cagtcattcg
    23761 atgacagcgt gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc
    23821 gtcagtggcg ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa
    23881 gccgtattga aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct
    23941 cctttgatgt acagaaaact cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc
    24001 atctggtggt tgcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc
    24061 gcgtcgaact ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc
    24121 agtttgagca ggcgaaagaa ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc
    24181 tgttggctga tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag
    24241 cccaggaagc cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga
    24301 tcgtttcggt attgcagagc gacccggaac agttcgaaca gctaaaagaa gattacgcgt
    24361 actctcagca gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc
    24421 agcgtcgtgc gcactttagc tattctgact cggcagaaat gctgagcggt aatagcgatc
    24481 tcaacgaaaa gctgcgtgaa cgtctggaac aggcggaagc ggagcgtact cgcgctcgtg
    24541 aagcgttgcg tggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga
    24601 aaagttctta cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata
    24661 tcggcgtgcg tgctgatagc ggggcagaag agcgggcgcg tattcgccgt gacgagctgc
    24721 atgcgcaact gagcaataac cgttcacgcc gcaatcaact ggaaaaagcg cttaccttct
    24781 gcgaagcgga gatggacaac ctgacccgca aactgcgcaa gctggagcgg gattactttg
    24841 agatgcgcga gcaggtagtg accgccaaag cgggctggtg tgcggtgatg cgcatggtga
    24901 aagataacgg cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg
    24961 atttgcgctc tatgtcggat aaggcattgg gtgcgctgcg tctggcggtg gcggataacg
    25021 aacatctgcg cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc
    25081 agttcttcgt ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta
    25141 ccgatgatcc ggtggaagct atcgaacaga tggagatcga acttagccgt ctgaccgaag
    25201 aattaacctc ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc
    25261 gcaaaaccat tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg
    25321 tatcgtttgg tcaggtgaac agcgtgcgcc tcaacgtgaa cgtgcgtgaa acgcacgcca
    25381 tgctgctgga tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc
    25441 gtctgacctt ctcggaagcg ttggcgaaat tgtatcagcg tcttaatccg cagattgata
    25501 tggggcagcg cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg
    25561 aaatggaagt tgaggttaac cgtggttccg atggttggtt gcgcgcagag tctggtgcat
    25621 tgtcgaccgg tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct
    25681 gggaagatga atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc
    25741 tcgatgaagc agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgagc
    25801 gtttgcaaat gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca
    25861 cctataaact ggtgcgtaaa gtcttccaga ataccgaaca tgttcatgtc gtcggcctgc
    25921 gaggatttgc gccgcaactc cctgaaacgc ttccaggaac tgacgaagcg ccttctcagg
    25981 cgagttaaaa ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg
    26041 cactaaaaaa gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg
    26101 taaaaacgta tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt
    26161 catactttcg tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc
    26221 agagtatgat aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct
    26281 gtcggcaatc agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga
    26341 tgagcctgaa gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact
    26401 gccgcaggcg caagccacgg caataatggc ggggatccag ccattgcctg aaggtgcggc
    26461 agaaaaagcc cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct
    26521 taaccagctt caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga
    26581 tgctgttaaa gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca
    26641 gtttaataaa tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt
    26701 agtgctctct gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg
    26761 cactcgctgg ctatatagca gtaaacctta tgcacttgca acgccgccgc tctcggtgat
    26821 taaccaatgg cagctggcgc tggataaagg tcaattgcct acgtttgttg caggactggc
    26881 accgcagcat ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac
    26941 caaaccgtgg ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga
    27001 cgtaccggcg ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat
    27061 tactctacct ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt
    27121 tgaaacagca gaaactaagc cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc
    27181 tgccgttcgt gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg
    27241 gcaaggattg ggggcagatg gtgctattgg cccggcaaca cgtgactggt taaacgtaac
    27301 gcccgcccag cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac
    27361 agagctttct accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa
    27421 cggcaatcag gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat
    27481 gatgagcagt gcccttaaca acgtagtggt aaacccgccg tggaacgtac ctccaactct
    27541 ggcacgcaaa gatattctgc caaaagtgcg caacgatccg ggatatctcg aaagccatgg
    27601 ctatacggtg atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg
    27661 gtctacaatc acggcctcga atttaccgtt ccgcttccag caggctccag gcccacggaa
    27721 ctcgctgggg cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac
    27781 gccgaaccac aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt
    27841 gaataaagct tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg
    27901 tatttctgat gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc
    27961 ggtgaatctc tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac
    28021 agatatttac aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga
    28081 acaattaatc aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga
    28141 ttgggggcga ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct
    28201 ttacgggcgg ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc
    28261 tgtagacttg attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg
    28321 tggcgttgca ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc
    28381 acgcccgcgc attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt
    28441 tttcgatggc agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga
    28501 ttaccgcgcg aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct
    28561 gcaagggttg ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttctattga
    28621 taccaacaat gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa
    28681 aggccaggcg atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc
    28741 gttatctatg cgcgcaggtg gtgtaggata ttatccacgt agtaactttg tgcatattga
    28801 taccgggcca gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat
    28861 cgtattattc cggtcaccgc attctcccag aactgttcat taatctggtg tgaacaaacc
    28921 cgtctgaccg cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat
    28981 gacagcggcc tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc
    29041 gcagcggcgg aactggcgca acattacggc gtgccggttt tgggcccgga aaaagaagat
    29101 gagttctggc tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag
    29161 ccgctgacgc cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact
    29221 ttacaggtgt tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg
    29281 gcaaagctgc tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc
    29341 ccgcgtggcg atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg
    29401 gatgacgtga catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg
    29461 cataatccct tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa aagcccgctt
    29521 ttaatgcggg cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc
    29581 gccttatccg gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcatg
    29641 ataagcgtag cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg
    29701 ggcttcattg tttttaatgc ttacagcact gccacaatcg cttcgcacag cggagccatg
    29761 ttatctggtg tcatcccggc cacatttacg cgaccagaag caaccgcata tacgccaaac
    29821 tcttcgcgca gacgcagcac ttgttctttt gtcaggccac tgaaggagaa catgccgttc
    29881 tgtttgatga taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac
    29941 aactgacgca tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca
    30001 cgtaacgcat cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta
    30061 gagtagttag cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg
    30121 tcggcagcaa ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag
    30181 taggaactgg caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca
    30241 tcttcttcca gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct
    30301 ttctcaactg agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt
    30361 gggttatggc agcagccatg gaacagcact acgtcgccag cctgagcttc gttcaggctg
    30421 ttaatcagtg catcgaagtc aagggtgtgg ttttctgcat cataataagc gtattcacga
    30481 acttccagac ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc
    30541 cacactcgct taacgctggt attttttgcc aggaaatcgg cagctacgcg tagtgcgcca
    30601 gtgccccccg gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta
    30661 ccaaacagca gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa
    30721 tttttggtgg tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcaatacc
    30781 ggggttttgc ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt
    30841 tcatcggcac gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg
    30901 ttctcaaaca tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac
    30961 gggagattta caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt
    31021 gagattgctc tggaaggcat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa
    31081 caaagaggtg tgctattaga actggtaaac gatacccaca gcaacggtgt cgtcagaacc
    31141 tacgcccagt ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat
    31201 gtttttgttg aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc
    31261 gataccttct acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa
    31321 atcgaactgg tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctgat
    31381 gtttgtaaat ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag
    31441 gtagatgttg ttcgcgtcat acttcagacc agtagcccac tgttcagctt ttttaccgtt
    31501 gccaagaagt tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat
    31561 accaaagcct tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagagcggcg
    31621 tgcagtgtca cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag
    31681 accaaagaag ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc
    31741 atcactgtat gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc
    31801 ataaaccaca ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc
    31861 cgcgaatgcc agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc
    31921 ctggaagtta tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc
    31981 tttaaaacca agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt
    32041 acccttggag aaataatgca gaccgacagc tttaccgtac agatctactt tgttgccatc
    32101 tttgttatag atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac
    32161 tgccagaata ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg
    32221 ccactgccgt caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt
    32281 gtctttatgt gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg
    32341 ctacaaagat aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa
    32401 ggaa
//
LOCUS       DAZFAH010000033        31222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFAH010000033 DAZFAH010000000
VERSION     DAZFAH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.033"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(152..439)
                     /gene="yebO"
                     /locus_tag="ACHJWB_001435"
     CDS             complement(152..439)
                     /gene="yebO"
                     /locus_tag="ACHJWB_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4313089.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(514..657)
                     /gene="mgrB"
                     /locus_tag="ACHJWB_001436"
     CDS             complement(514..657)
                     /gene="mgrB"
                     /locus_tag="ACHJWB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4313090.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            816..1055
                     /gene="yobH"
                     /locus_tag="ACHJWB_001437"
     CDS             816..1055
                     /gene="yobH"
                     /locus_tag="ACHJWB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4313091.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1198..1989)
                     /gene="kdgR"
                     /locus_tag="ACHJWB_001438"
     CDS             complement(1198..1989)
                     /gene="kdgR"
                     /locus_tag="ACHJWB_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4313092.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2166..3539
                     /gene="yebQ"
                     /locus_tag="ACHJWB_001439"
     CDS             2166..3539
                     /gene="yebQ"
                     /locus_tag="ACHJWB_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313093.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3585..4466)
                     /gene="htpX"
                     /locus_tag="ACHJWB_001440"
     CDS             complement(3585..4466)
                     /gene="htpX"
                     /locus_tag="ACHJWB_001440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4313094.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4658..6706)
                     /gene="prc"
                     /locus_tag="ACHJWB_001441"
     CDS             complement(4658..6706)
                     /gene="prc"
                     /locus_tag="ACHJWB_001441"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4313095.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYVLSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6726..7424)
                     /gene="proQ"
                     /locus_tag="ACHJWB_001442"
     CDS             complement(6726..7424)
                     /gene="proQ"
                     /locus_tag="ACHJWB_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4313096.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7521..8018)
                     /gene="msrC"
                     /locus_tag="ACHJWB_001443"
     CDS             complement(7521..8018)
                     /gene="msrC"
                     /locus_tag="ACHJWB_001443"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4313097.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8148..9431
                     /gene="yebS"
                     /locus_tag="ACHJWB_001444"
     CDS             8148..9431
                     /gene="yebS"
                     /locus_tag="ACHJWB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4313098.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLHRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9400..12033
                     /gene="yebT"
                     /locus_tag="ACHJWB_001445"
     CDS             9400..12033
                     /gene="yebT"
                     /locus_tag="ACHJWB_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4313099.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12113..13552
                     /gene="rsmF"
                     /locus_tag="ACHJWB_001446"
     CDS             12113..13552
                     /gene="rsmF"
                     /locus_tag="ACHJWB_001446"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4313100.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALNGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13670..13906
                     /gene="yebV"
                     /locus_tag="ACHJWB_001447"
     CDS             13670..13906
                     /gene="yebV"
                     /locus_tag="ACHJWB_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4313101.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            14011..14202
                     /gene="yebW"
                     /locus_tag="ACHJWB_001448"
     CDS             14011..14202
                     /gene="yebW"
                     /locus_tag="ACHJWB_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4313102.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14203..14859)
                     /gene="pphA"
                     /locus_tag="ACHJWB_001449"
     CDS             complement(14203..14859)
                     /gene="pphA"
                     /locus_tag="ACHJWB_001449"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4313103.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15255..15596)
                     /gene="yebY"
                     /locus_tag="ACHJWB_001450"
     CDS             complement(15255..15596)
                     /gene="yebY"
                     /locus_tag="ACHJWB_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4313104.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15609..16481)
                     /gene="copD"
                     /locus_tag="ACHJWB_001451"
     CDS             complement(15609..16481)
                     /gene="copD"
                     /locus_tag="ACHJWB_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4313105.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16485..16859)
                     /gene="yobA"
                     /locus_tag="ACHJWB_001452"
     CDS             complement(16485..16859)
                     /gene="yobA"
                     /locus_tag="ACHJWB_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4313106.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16998..17228
                     /gene="holE"
                     /locus_tag="ACHJWB_001453"
     CDS             16998..17228
                     /gene="holE"
                     /locus_tag="ACHJWB_001453"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4313107.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17330..17986
                     /gene="yobB"
                     /locus_tag="ACHJWB_001454"
     CDS             17330..17986
                     /gene="yobB"
                     /locus_tag="ACHJWB_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4313108.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            18010..18672
                     /gene="exoX"
                     /locus_tag="ACHJWB_001455"
     CDS             18010..18672
                     /gene="exoX"
                     /locus_tag="ACHJWB_001455"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4313109.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18669..20729)
                     /gene="ptrB"
                     /locus_tag="ACHJWB_001456"
     CDS             complement(18669..20729)
                     /gene="ptrB"
                     /locus_tag="ACHJWB_001456"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4313110.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20938..21597)
                     /gene="yebE"
                     /locus_tag="ACHJWB_001457"
     CDS             complement(20938..21597)
                     /gene="yebE"
                     /locus_tag="ACHJWB_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4313111.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSTDQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEGQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21924..22280)
                     /gene="yebF"
                     /locus_tag="ACHJWB_001458"
     CDS             complement(21924..22280)
                     /gene="yebF"
                     /locus_tag="ACHJWB_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4313112.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCEAGMAEYQRR"
     gene            complement(22347..22637)
                     /gene="yebG"
                     /locus_tag="ACHJWB_001459"
     CDS             complement(22347..22637)
                     /gene="yebG"
                     /locus_tag="ACHJWB_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4313113.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22771..23949
                     /gene="purT"
                     /locus_tag="ACHJWB_001460"
     CDS             22771..23949
                     /gene="purT"
                     /locus_tag="ACHJWB_001460"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4313114.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(24005..24646)
                     /gene="kdgA"
                     /locus_tag="ACHJWB_001461"
                     /gene_synonym="eda"
     CDS             complement(24005..24646)
                     /gene="kdgA"
                     /locus_tag="ACHJWB_001461"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4313115.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24683..26494)
                     /gene="edd"
                     /locus_tag="ACHJWB_001462"
     CDS             complement(24683..26494)
                     /gene="edd"
                     /locus_tag="ACHJWB_001462"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4313116.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26729..28204)
                     /gene="zwf"
                     /locus_tag="ACHJWB_001463"
     CDS             complement(26729..28204)
                     /gene="zwf"
                     /locus_tag="ACHJWB_001463"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4313117.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28541..29410
                     /gene="hexR"
                     /locus_tag="ACHJWB_001464"
                     /gene_synonym="yebK"
     CDS             28541..29410
                     /gene="hexR"
                     /locus_tag="ACHJWB_001464"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4313118.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29538..30980
                     /gene="pyk"
                     /locus_tag="ACHJWB_001465"
     CDS             29538..30980
                     /gene="pyk"
                     /locus_tag="ACHJWB_001465"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4313119.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31040..>31222)
                     /locus_tag="ACHJWB_001466"
     CDS             complement(31040..>31222)
                     /locus_tag="ACHJWB_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFO4313120.1"
                     /translation="DSIKTWVQTQYSKDSGLPMAVIGYSVAQEAGMRRLASYLDLHSG
                     YPCIHFTGGCDYDWIE"
BASE COUNT         7558 a         8033 c         7664 g         7967 t
ORIGIN      
        1 tctgatatcc cgttacgcaa ataaataata tgataattat ttgaagtcag accagagttt
       61 taatttgaag cagttgcacc atggcagtgc gtataatcag gatgaattga gtaacccttc
      121 aacgacgggg ttactcaaga ttgaggaagg attatcgttc ggcgaccaga cgaataatat
      181 cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc tcaggttcgt
      241 ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc agcaacgcct
      301 caagcagttc aatctgttcg ttagtccgag aactggcacg attgataaaa aaccacaaga
      361 tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
      421 aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg
      481 agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
      541 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
      601 aagcagcaag caagccaaca ccacgacaac cagaacgacc catcgaaact ttttcactcc
      661 aatctccgct tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
      721 actatgtcga tattcgtgct ttttcggaat gagtagcttg cttcagcgca tattgccgct
      781 acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta
      841 tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
      901 gcaaagagaa tgccgcagga ttaggtttgc aatgtaccta tctgactgcc agaggcacca
      961 gcacggtaca atacttgcac actaaaagtg gtttcttggg gataacagat tgcccactgt
     1021 tacgtaaaag caatatcgtg gtcgataatg gttaagggta ataaataacc agattctcac
     1081 tcatgagtaa aatattctta tagcaagcgc gtttaatata acgcaccttc aactttattt
     1141 ttctcatgct aaataaagaa cctgtctgtt tgatattccg acaggttctt actctcatca
     1201 gaacggatag tcgtgataac ccatttgggc agaaattttg cgcgctgcgg tatgcaacat
     1261 tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg agatgctcaa
     1321 accggcaatg accacgccaa agcgatcaaa taccggtacc gcaatgcatc gcagcccttc
     1381 ttcctgctct tcgttatctt cgccataccc ctgctcgcgc acctggtcca gaacgggtaa
     1441 taacgcttct gtactggtga tggtccgctc ggtactgcgt ttatactcca cgccctcaag
     1501 aatttgcttc acttcatcgc gatcgcgcca tgccagcagt accttaccaa tcgcggtgct
     1561 gtacagcgga ttacgacgcc caatccgtga atacatgcgc aaattgtaca tagagtcaat
     1621 tttgtgaatg taaacaatac tgtcttcgtc cagtgcgccg aggtggatag tttctttggt
     1681 caggcgggag atctcacgca tctggatatc tgcgctacga attaaatcga cgttttgtaa
     1741 cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa tatttctccg actccccttc
     1801 ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag cgataaacgg tgctttttga
     1861 catcatgacg cgctgcgaca gctcggttat ccctatttcg cgctcttcac ccagcgcctg
     1921 caaaatgcca aaaactttta gcacggaaga tacagaatca ggctgtttat ccagatctgc
     1981 gttagccatt tatcacctca ttgcaagtgt tttataaaaa tcagaactgt tttttattat
     2041 aatttcgcac cagggtggtc gcaatccatc ttttgccggt tagttacaat tctgcgacat
     2101 ccaccgtgaa tatcagtgct agaatcatac ccctgttgat tattcaccaa agatataaaa
     2161 ttcctatgcc aaaagttcag gccgacggcc tgccattgcc ccagcgatac ggtgcgatat
     2221 taaccattgt gattggcatt tcgatggctg tccttgacgg cgcaatcgcc aacgtcgccc
     2281 tgccaacaat cgccacggac cttcatgcca cgccagccag ttccatctgg gtagtgaacg
     2341 cctatcaaat cgccattgtc atctccctgc tctcgttttc gtttctgggc gatatgtttg
     2401 gctatcgacg tatttataaa tgcggtctgg tcgtttttct gttgtcttca ctgttctgcg
     2461 ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt ttcggcggtg
     2521 cagcgttgat gagcgttaat accgcactta tccgcctgat ctatccacaa cgttttctgg
     2581 ggagagggat gggcataaac tcgtttattg ttgccgtctc ttctgctgcc gggccgacaa
     2641 ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt atttttaatc aacgtaccgt
     2701 taggtattat cgccctgctt ctggcgatgc gttttctgcc acccaatggt tctcgcgcca
     2761 gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc gttaaccttc ggcctgctta
     2821 tcactgcgtt gagtggtttc gctcaggggc aatcgctgac gttaattgct gcggaactgg
     2881 tggtaatggt tgttgttggt attttcttta ttcgccgcca gctttctctt cccgtaccgc
     2941 tgctaccggt ggatttactg cgtatcccgc tgttttcact ttctatttgc acatctgttt
     3001 gctctttctg cgcacaaatg ctggcaatgg tttccctgcc cttttacctg caaaccgtgc
     3061 tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc gtggccgtta gcaacgatgg
     3121 tgatggctcc gctggcaggc tatttgattg aacgcgtaca tgcaggattg ctgggtgctt
     3181 tagggttgtt catcatggct gcggggcttt tttccctggt tctgctgccc gcgtcacctg
     3241 cggatatcaa tattatctgg ccgatgatct tatgtggcgc tggatttggc ttattccagt
     3301 cacccaataa ccacaccatt attacctccg ctccgcgcga acgtagcggt ggggccagtg
     3361 gcatgttagg aacggctcgt ctactgggtc agagtagtgg cgcggcgctg gtggcgctga
     3421 tgctaaatca gttcggtgat aatggtacgc acgtttcgct gatggctgcg gctattctgg
     3481 cagtgattgc tgcctgtgtg agtggtttac gtatcactca gccacgatcc agggcataaa
     3541 aaaagcgcgt cgatcaggac gcgcttttta gtatttactt catattactt caggtattca
     3601 cccgtacgca gagcttcaat tcgtttatcc agcggcgggt gagtcatgaa caactcactg
     3661 agcgatttcg acttaccgtt aatgcagaaa gccatcatgc tggttgcttc ttgcggttca
     3721 tagctggttt tcaggcgctg cagcgcggca atcattttct cgcgaccaac cagttttgcc
     3781 gaaccggcat cagcatggaa ttcacgatga cgcgagaacc acatggtgat aatgctcgcc
     3841 agaataccaa acaccagttc cagaaccgtt gcaaccgcaa agtagatcag cgggttgccg
     3901 ttgctctctt caccttcatc acgatttccg cccataaaac ccgcggcaag ctgcgccaga
     3961 atacgggaaa taaagataac gaaggtgttc accacgccct gaatcagcgt catggtaacc
     4021 atatcaccat tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc ttcatccggg
     4081 ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg gcgcgcaccg
     4141 gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca cttgcggcat agcgatccct
     4201 gcctgacgag cctgggttgc tacagtatta accagccaac gttccctttc gttacgcggt
     4261 tgctcgatca cttccccgcc aacagatcgt aatgccatcc atttggacat cagaagcgaa
     4321 acgaaggaac caccaaaacc gaacagcaag gccatgatca tcagcccctg aacgctgctc
     4381 gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga ccattacggc caggttcgtt
     4441 agcaggaaga gcgcgattcg catcataatt ttctttttac ctcagtttaa caaaacgcaa
     4501 tatgcgatac ccacatcgta tgggttacgc gactattttc aagtctggat agtgcgtaag
     4561 tcaccagaaa gacacaactt tacattttgt agcatctgat ttacggcatc ttgccgctgt
     4621 taaaaaatca ggcacaattt cttgtgcctg attgatatta cttgacggga gcgggttgtt
     4681 ccgcgggtct ggctttttca agcttcgcca gatcgagtgc gatattcacc gtctcatcca
     4741 gataaggatc cggctcctgg taatcttttg gtagatcatc cagcttcttc aactccggtt
     4801 taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca tcttcattat
     4861 tctctttctc acgcacagcg taattcagag aaacgatatt gcgcttgtcc ttcatagcgt
     4921 tgaagcgcgc gatatccttc atgatgttct ggaactcagg atctttcgcg atacgcgcat
     4981 tatgttcctt cagtagctcc ggttcaaagg ccgttaaatc gcctgatttc acataagtcg
     5041 cagcatcaat gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc gtttccgttt
     5101 cttcattacc cgtcggcatg atgatgtctg gcgttacgcc tttacgttgc gtactgccgc
     5161 cgttaacgcg atagaatttc tggatcgtgt actgcacaga acccagcgct ggccattcag
     5221 gacgtaacat ctgatcgtaa atacggttca atgaacggta ttgctgaacg gtgcctttac
     5281 caaacgtcgg ttcacccaca accagcgcac gaccgtaatc ctgcattgcc gcggcaaaga
     5341 tttctgaagc cgaagcactg aaacggtcaa ccagtaccac cagcgggcct ttatagaaca
     5401 cctgcccgtc ggtatcgctg tcttcacgaa ccttgccgtt gttatcgcgg acctgaacaa
     5461 tgggacccgc aggaataaac agacctgaga gcgataccgc ttcggtcaac gccccaccgc
     5521 cattgctacg caggtcgatg atcacactgc tgacattctg tttttccagt ttctgcagtt
     5581 gaactttgac atcgtctgtc aaacccacat agaagcccgg aatatccagc acgccgactt
     5641 tctctttacc gacggtcttc accgacattt taaccgcgcg gtcttcgaga cgaatacgtt
     5701 cacgggtcaa tgttacagtg cgggtcttgg tccctttacc agcaggtaaa atttccagac
     5761 gaactttact gcccttcggc cctttaatta aggcaaccac atcatcaaga cgccagccaa
     5821 tcacgtcaac catcggcttg cctgtttgac caacaccgac aattttgtca ccaacgctga
     5881 tagctttact cttcgctgcc ggaccacctg ccaccatcga attgataacg gtgtagtcat
     5941 catccatttg cagcactgcg ccaatacctt ccagcgacaa gctcatttca gtgttgaact
     6001 gttcggtatt acgcggggaa agatagttgg tatgcgggtc gatttcacgc gcaaacgccg
     6061 tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga atggcaaatt
     6121 tgtagcggcg tgtcagggtt tcacgaattt ctttatccgt ttttcctgcc agcttcaggc
     6181 ttaactcgtc gaatttgact ttactgtccc acagcgcgtt caactcagcc tcgtttttcg
     6241 gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa tccatcggct
     6301 tttccagtac cgacaaaacg tactggtaac gctcaaaacg gcgcttttgc gccagattgt
     6361 agagatcgta gaaaacgtcg agtttgcctg aacgcagttc atcgcctaac tcggttttct
     6421 ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacgtt gtggctgtaa tcgagcagat
     6481 tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac tggcgataat
     6541 gagaacgggt gaagcgcgac gttacgcgct cacttaccgt cgcatgctgc gtctcttcct
     6601 ttaataccgg aatttgatca gcacgcgtga tatcttctac agcgaaggtc tggcctgcta
     6661 ttgcaagcag gccagctaac gcggtaagcc taaaaaacat gttcatgcct ggcccggcct
     6721 ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag acatacccga attcagctgg
     6781 acgcggacac cgtctttggt gatttctaat acggtggcat ccatcgcgtt ttgacccgct
     6841 ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt cagaaaccgg ggtgtgctgt
     6901 tcttcgcgag gtgcttttac tgtttttggc gctttctcta ccggcttttg cgcacgaggt
     6961 ttacgttcag cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg ttcgcggcgc
     7021 ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc gttttttcgc ttgctgttca
     7081 gcacgctgtg cctgaacacg cgctttcgct tcttcaagct gcttgcgagc atgctctaca
     7141 tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat cgacacgcgt tgcgccgggt
     7201 ttaacaccgt aaagataacg ccagctcgaa gtgtagagac gtaaagcgga tcgtaattgc
     7261 gttttgctca ggttcatttc cccggcaaca cgatcgacca aatcctgaaa aataccgatt
     7321 ttcagcggac gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc ggccagaaac
     7381 gcgattactt ctttactgct attcaactta ggttgatttt ccatgaaatt tcctgattac
     7441 aacggacgta gccaacaagc gcaggcatga acaggcgtca ttataatgac gctaccagta
     7501 aatgctacgt tatccgttga ttatcctgcg acgctcgcaa agaatttttt gtaatccgtc
     7561 gttgcaagca ctttttcaag ctgtgccaca agctgacgta agccttgctc gtcctcgtct
     7621 gtaaagcgag cgaagacggt actatcgatg tcaagaacac caataatctg atttttcacc
     7681 accagcggca gaacaatttc agaattactc gccgcatcac aggcaatatg cccgtcaaac
     7741 gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa ccgcagtgcc gcacaccccg
     7801 cgcccgacgg gtatccggac acaggcaatt ttgccctgaa atggtccgag taccagtgta
     7861 tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag tgagacgctc gtataacaac
     7921 gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc ccgccatcag cgcgttaaag
     7981 tcgcgattta aatccgcgta aaattctgtt ttgttcatta tataatcact tggttgtctt
     8041 acctggaact gccagcctat taaaataagc attaaatgcg ttaatgctca agatcattcc
     8101 caacatgggt taatattaat gttaattctt attacatttg gcacgtcatg gctcttaaca
     8161 caccacaaat tacgccgaca aaaaagataa cagtgaggtc aatcggcgag gaactgccgc
     8221 gtggtgatta ccaacgttgc ccgcaatgcg acatgctgtt tagcctgccc gagataaatt
     8281 ctcatcagag tgcctattgt ccgcgctgtc aggcaaaaat tcgtgacggg cgcgactggt
     8341 cgctaacgcg cctggcggca atggctttca ccatgctgtt gctgatgccg tttgcctggg
     8401 gagaaccgct gttgcatatc tggctgttag gtattcgtat cgacgccaac gtcatgcaag
     8461 gcatctggca aatgaccaaa cagggcgata cgataacggg ggcgatggtc tttttctgcg
     8521 ttattggtgc ccccctcatt ctggtgtcct ccatagcata tttatggttt ggtaaccgac
     8581 tgggaatgaa tttacgtccg gtactgctga tgcttgagcg tcttaaagag tgggtaatgc
     8641 tggatatcta cctggtcggc attggcgttg cttctataaa ggtacaggat tatgcccata
     8701 tccaggctgg tgtcggcttg ttctcttttg tggcgttggt gattttaacg acggtgacgt
     8761 tgtcacatct taatgttgag gagctgtggg agcgatatta tccgcagcgc cccgctacgc
     8821 gtagggacga gaaacttcgt gtctgtcttg ggtgccattt taccggctat cctgatcagc
     8881 gtggtcgctg cccgcgttgc catatcccgc tacgcctgca tcgccgtcat agtctgcaaa
     8941 aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt accagccaac ctattgccta
     9001 tttctatcat ttatctgaac ggaggacggc aggaagatac aattctttcc ggaattatgt
     9061 cgctggcaag tagcaacatt gcggttgcgg gaatcgtgtt tatcgccagt attctggtac
     9121 cgtttactaa agtgatcgtc atgttcactt tactgttgag cattcatttt aaatgccagc
     9181 aaggtttacg cacacgcatt ctgttactgc ggatggtgac ctggattgga cgctggtcga
     9241 tgctcgacct gtttgtcata tctttaacca tgtcgctgat taatcgcgat cagatcctcg
     9301 cttttactat gggaccggct gcgttttatt ttggcgcagc cgtaattttg actattcttg
     9361 ctgtggaatg gctggacagc cgcttacttt gggatgcaca tgagtcagga aacgcccgct
     9421 tcgacgactg aagcgcagat taaaaataaa cgccgtatct cacctttctg gctgctgcct
     9481 ttcatcgcgc taatgattgc cggttggctg atttgggaca gttatcagga ccggggtaat
     9541 accgtcacca tcgactttat gtcggcggat ggtattgttc cgggccgtac gcctgttcgt
     9601 tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc tcagcgacga tcttcgtaag
     9661 attgaagtca aggtcagcat caagtccgat atgaaagatg cgctgcgcga agagactcaa
     9721 ttctggctgg tgacgccaaa agcatcgttg gcaggtgtct ccgggctgga cgccctcgtc
     9781 ggtgggaact atatcggcat gatgccgggt aaaggtaaag agcaggatca ctttgtcgca
     9841 ctcgataccc aaccgaagta tcggctggat aacggcgatc tgatgatcca cctgcaagct
     9901 cccgatctcg gttcgctgaa cagcggttca ttggtctatt tccgcaagat cccggtggga
     9961 aaagtctacg actatgccat caatcccaac aagcaaggcg tggtgattga tgtcctgatc
    10021 gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt tctggaacgt ttccggcgtt
    10081 gatgccaacg tcagtgtcag tggcgcgaag gtgaaactgg aaagtctggc ggcactggtt
    10141 aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac ctgccgaggc ggaagatacc
    10201 tttggtctgt atgaagatct ggcccacagc cagcgtggcg taataataaa actggaactg
    10261 ccgagtgggg ccggattgac cgccgactcg acgccgttaa tgtatcaggg gctggaagtc
    10321 ggacagctaa ctaaactgga tttaaatcct ggtggtaaag tcactgggga aatgacagtt
    10381 gatcccagcg tcgttaccct gcttcgtgaa aatacccgca tcgaattacg taatccgaaa
    10441 ttatccctta gcgatgccaa tctcagcgcc ctgctgactg gcaaaacctt cgagttggta
    10501 cccggcgatg gcgagccacg caaagagttc gttgttgtgc caggcgaaaa agcactgctg
    10561 catgaacctg atgttctgac gctgaccctg accgcgccgg aaagttacgg tattgatgct
    10621 ggtcagccgc tcattcttca cggcgtgcag gtaggccagg ttatcgatcg taaactcacc
    10681 agcaaaggcg tcacctttac cgtcgccatc gagcctcagc atcgagaact ggtaaaaggc
    10741 gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg tggggctgga tggcgttgag
    10801 tttctcggtg ccagcgcctc agaatggatt aacggcggga tacgtattct gccgggcgat
    10861 aaaggcgaga tgaaagccag ctatccactg tatgccaatc tggaaaaagc gctggagaac
    10921 agccttagcg atttacccac cacaaccgtg agtttgagtg cagagacgct gccggatgtg
    10981 caggcaggat cggtagtgct gtaccgtaaa tttgaagttg gtgaagttat taccgtgcgt
    11041 ccgcgagcta acgcgtttga tatcgatctg catattaagc cggagtatcg caaccttctg
    11101 accagcaata gcgtgttctg ggcagaaggc ggggcgaaag ttcagctgaa tggtagtggc
    11161 ctgaccgtac aggcatcccc gctctccaga gcattaaaag gggctattag cttcgataac
    11221 ctcagtggtg ccagcgccag tcagcgtaaa ggcgacaaac gtattctgta tgcttccgaa
    11281 acagcggccc gtgcggttgg tgggcagatt acgcttcacg ctttcgatgc cggaaaactg
    11341 gcggtcggga tgccaattcg ctatctcggt attgatatcg ggcaaatcca gacgctggat
    11401 ctgattaccg cgcgcaatga agtacaggca aaggcggtgc tctatccgga atatgtccag
    11461 acctttgctc gcggtggtac gcgcttctca gtggtcacac cgcaaatttc ggcagctggc
    11521 gttgagcatc ttgatactat cctccagccg tatatcaacg tcgaaccagg ccggggcaat
    11581 cctcgccgcg actttgaatt acaagaggcc accattactg attcgcgtta cctggatggc
    11641 ttaagcatta ttgttgaagc gccggaagcc ggttcgttag gtattggtac gcctgtgctg
    11701 ttccgtggtc tggaagtcgg tacggttaca ggaatgacgc tggggacatt gtcagatcgc
    11761 gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa caattccgtc
    11821 ttctggttgg catcgggtta cagtctggac tttggtctga cgggcggcgt agtgaaaacc
    11881 ggcaccttta accagtttat ccgtggcggc atcgccttcg ccacgcctcc gggtacgcca
    11941 ctggcaccga aagcccagga aggcaaacac ttcctgttgc aggaaagtga accgaaagag
    12001 tggcgtgaat ggggaactgc gcttcccaaa taatgcccac tgctccggcg tgcctgcgcc
    12061 ggagcgttta tgctaaactg cgcacctgtt tttttgccag tggtacatgc tcgtggccca
    12121 acacaccgtt tatttcccgg acgcctttct gacacaaatg cgcgaagcga tgccttcgac
    12181 gctctcattt gatgattttc ttgccgcctg tcagcgcccg ttgcgccgca gcattcgcgt
    12241 taatacgctg aaaatctccg ttgctgattt cctgcaatta accgctcctt atggctggac
    12301 gcttacgcca attccgtggt gtgaagaagg tttctggatt gaacgcgaca atgaagatgc
    12361 attgccattg ggtagtaccg ccgagcattt aagcggcctg ttttatattc aggaagccag
    12421 ttcaatgttg cccgttgccg ccttgtttgc tgacgataat gcaccacagc gggtgatgga
    12481 tgtcgcagct gcgccaggct ccaaaacgac gcaaattgcc gcgcggatga ataacgaagg
    12541 ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa gtgttacatg ccaatatcag
    12601 ccgctgtggc atcagtaatg ttgcgctcac acattttgat ggccgcgtgt ttggtgcggc
    12661 agtgccagaa atgttcgatg ccattttgct ggacgctccc tgctctggcg aaggcgtggt
    12721 gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa agcaatcagg aaatcgcagc
    12781 tacacaacgg gagcttatcg acagcgcctt tcatgcatta cgtcctggtg gtacgctggt
    12841 ttactcgacc tgtaccttaa accaggaaga aaacgaagcc gtttgcctgt ggctgaaaga
    12901 gacttacccc gacgcagtag agtttttacc acttggcgat ctcttccctg gtgcaaacaa
    12961 agcgctgacc gaagaaggct ttttgcatgt tttcccacaa atttacgact gcgaaggctt
    13021 cttcgttgct cgtctgcgta aaactcaggc gatccccgcc ttacccgccc ccaaatacaa
    13081 agtgggcaat ttcccgttca gcccggtgaa agatcgcgaa gccggacaaa ttcgtcaggc
    13141 ggctgcaagt gttggcttaa actgggatgg aaacctgcga ctctggcaac gcgacaaaga
    13201 actgtggttg ttcccggtag gcattgaagc cctgaacggt aaagtccgat tttctcggtt
    13261 ggggattaaa cttgccgaaa cgcataacaa aggttatcgc tggcagcatg aagcggttat
    13321 tgcccttgcc tcccccgaca atgtgaacgc ttttgaacta acaccgcagg aagcggaaga
    13381 gtggtatcgc gggcgcgatg tttacccgca agccgcgcca gtagcggatg atgtattggt
    13441 tacttttcag catcagccga ttggtttagc caaacgaatt ggttctcgac tgaaaaacag
    13501 ctacccgcgt gaactggtgc gcgatgggaa actttttacc ggtaacgcct gacagcgcac
    13561 aaaaaaagcg cactttttga ctggcacatt cggctgcctc aactaggctg aaaaatggtg
    13621 cgatcgaact ggtcgtacca caaccggcag ctaaatggag agcacgaaga tgaaaaccag
    13681 tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta cacggtgaat cgccgggtga
    13741 tcgaacgtta accattcctt gtaaatctga ccccgattta tgtatgcaac tggatgcctg
    13801 ggatgctgaa accagtatcc ctgccctgct aaatggcgaa cactctgtcc tttaccgtac
    13861 ccgttacgat caacaatctg atgcctggat tatgcgtctt gcctgatcca aaaagaaccc
    13921 gtcggcatgg cgggttattt gtcctggtta ttcccccgtt gtaaaatctc tcctaaactt
    13981 aacggtacgg caccacactt cggggatgaa atgttcgcgc tggtactttt tgtttgctac
    14041 ctggatggcg gttgtgaaga tattgttgtg gatgtctaca acacggaaca gcagtgtctt
    14101 tattctatga gcgatcaacg gatccgccat ggcggttgtt ttccgattga ggattttata
    14161 gatggtttct ggcgaccagc acaggagtac ggtgattttt aattattgca gttgcacaag
    14221 agtcagctcg cccccaaaga cagcgccggt atcaatataa tgcaggttgc caatatccac
    14281 gcgatgtcgc aacggtgtat gaccaaacca gaaatgatca gcacctttaa ttccctgccc
    14341 ttttgggcgt tcacctaatc gcgagcggct ccacaagacc tgatgcaaat caacgtcctt
    14401 ttgccattca taaacatcat ctggataatc ggcatgagca ataacatgct tgccggtgcg
    14461 actgtgtact tcaagaataa agggcaaatg ctgacatttt tccagcgccg ttttcgcttg
    14521 tttctgttga ttatctgcca gcgcaataaa ccagtcgccg ccattcatca accacaaaga
    14581 catctgctgg gatgccagcg catccatcgc catctgttca tgattgcctc ttaccgcaca
    14641 aacccaatgt tgttccagta actgcagaca acgtaaactt tgcggcccac gatcgataac
    14701 gtctcccact gagataagta aatctcgcca cggatcaaaa cgacaatgcc ataatttgcg
    14761 gcgcaactgc tcaagacaac cgtgtatatc gccagaaagc cagatatgtc gccattgatg
    14821 acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc aatattttcc tcccgcgcta
    14881 aagatcacat aatcttaaca agaatgttaa aaaacgctgg actcagacag tagagtgtgt
    14941 gttatggttg actataaagt cagcgaagga aatgcttctg gcttttaaca gataaaaaga
    15001 gaccgaacac gattcctgta ttcggtccag ggaaatggct cttgggagag agccgtgcgc
    15061 taaaagttgg cattaatgca ggcttagttg ccttgccctt taagaataga tgacgacgcc
    15121 aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg tggtggtcat cagctgaaat
    15181 gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt tttttattgg aaatcaaaag
    15241 gctattttag gtaattaaca gagtttttca gctcgttcta taaacggtgc cagactcatt
    15301 ttttcgccgg gattgttagg atcatcaatc tgaatcaccg aaatgggttg ggcattggtc
    15361 ttcccactgg caacttcctt ttgtgcgata tcgtttaaag gatactgcac gagggtactc
    15421 ggattaataa catacaaagc attacccggt cggcaagtca gcatcacctc ttcgcgatta
    15481 aacgcccatt tgtctttacc cacttcaaaa cgactgacgg taatcacctg cggtgcagcc
    15541 agcgccgctg cagaactggt gagtaacaga aacgccagaa tacttttttt catcattttc
    15601 gtaatccatc aaaaaggttc gagagtcgca agcaggctga tgatcaccag cactaccgca
    15661 cctatcgccc attcgagttt agtcatccac acaaaccagg gtgccgcacg atcttcatcc
    15721 tgcctcatgc gcggtacaag aacataacga ttagccagcg caatgaccac cataatcatc
    15781 accaggatgg ctttgagcaa taagagttgg ccccagtaag tcgtaagcgt ggggggaaat
    15841 ccggtaatca acaatgcatt aagcacgccg cttgccagta cgccgatcac cgcaaaatgc
    15901 ccgcaccagg aaaaacgcat cagcgcctga atggcctggt gtcgccaacg gcctttgatg
    15961 agttgcatac accagagcac cggcagtaat cccccaaacc acgccgccgc acaaatcagg
    16021 tgaatcgcgt gattagtctg atggattttc gccgttaccc cttcattcag cgtcgcatgc
    16081 cccactcccg ccagcagaat aaattgcgcg gtagtgagca taaacagcaa acgtggcata
    16141 ttccgcggtt gcataagggc gacgatcaac gttacgaggg cgagaacaat ttgccataac
    16201 cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa tgtttggcga aaatacatct
    16261 gtccatcccg ttcccatcaa cccgccctga acggcgagca ttgcagtggc actgatcaaa
    16321 ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct tcgccagcaa gcgacgaatc
    16381 gtcaaggggg ccagccaggc accgtacatt gcgaagccaa aaaccagcat cagcgaggtg
    16441 aaatggataa atcgcagcgc gatccaggta aacgccagca tgatttattt cacgctaaag
    16501 gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa catgccagtc aacggtgtag
    16561 gttccgggtt tcagcgaatc ggccagtgga acaatcagtt gcttttggtc ctgctcattt
    16621 cgcttcgccg gtaatgtttt aatattttcg ttttttggcc ccgtgatttt tgcaccactg
    16681 aatcctgttt caacaccttc cgagaagttt aaagtgattg cctgcggtgc agctgtcact
    16741 tgcgcgtttg ccgcaggata ctgatgcgtt aaatgcgcat gtgcccagac ggaaggtgtc
    16801 accaacgaag tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt tgaagccata
    16861 ttatctattc ctttttgtaa taactttttt acagagcata accttgtcta atgtctgagt
    16921 cgaggatcat caattccggc ttgccatcct ggctcactct tagtaacttt tgcccgcgaa
    16981 tgatgaggag attaagaatg ctgaagaatc tggctaaact ggatcaaaca gaaatggata
    17041 aagtgaatgt cgatttggcg gcggccgggg tggcatttaa agaacgctac aatatgccgg
    17101 tgatcgctga agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg tttcgcgagc
    17161 ggcttattgc ccaccgtttg gcttcggtca atctgtcacg tttaccttac gagcccaaac
    17221 ttaaataaaa cttatacaga gttacacttt cttacataac gcctgctaaa ttatgagtat
    17281 tttctaaacc gcacccataa tttgcagtca ttttgaaaag gaagtcatta tgtcgttctg
    17341 gaaagttgca gcagcgcaat atgagcctcg taaaacctca ctcaccgaac aggtagctca
    17401 tcatctggag tttgtgaggg ccgccgccag gcaacaatgt cagctgttag tttttccatc
    17461 gctttcttta ctggggtgtg attattcgcg acgtgccctt cctgccccac ccgatctctc
    17521 actgttagac ccgctttgtt atgccgcaac gacctggcga atgaccatca ttgccggcct
    17581 tcccgttgaa tataacgatc gctttattcg tggaattgcg gtgttcgcac cgtggcgaaa
    17641 aacgcctggg atctaccatc agagtcatgg tgcatgtctg ggccgtcgtt ccagaaccat
    17701 cacggtggtc gatgaacaac cggaaggtat ggatatggac ccgacctgtt cactgtttac
    17761 gacagggcaa tgtctgggag aacccgacct gctggcgtcg gcccgtcgct tacaattttt
    17821 ttcacatcag tactcgattg ctgtgctgat ggcaaatgcc cgtggtaaca gtgcactatg
    17881 ggatgaatat ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt tagtcggtca
    17941 gcgttcatca cagggttggc aaggcgatat cattccatta cgctaggctt tttcggcctg
    18001 gagcatgcca tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg agggatcgtt
    18061 gagattgcct ctgttgatgt cattgacgga aaaatcgtca accccatgag ccacctggtg
    18121 cgccccgatc gtcctattag tccacaagcg atggcgattc atcgcatcac cgaagccatg
    18181 gtcgccgata aaccgtggat tgaagatgtg atcccacact attacggcag tgaatggtat
    18241 gtcgcgcaca acgccagctt tgaccgccgc gtactgcctg agatgcccgg tgagtggatt
    18301 tgcaccatga aactggcccg tcgcttgtgg ccaggaatca agtacagcaa tatggcgtta
    18361 tataaaacac ggaagctcaa tgtacagacg ccgccgggcc tgcatcatca ccgcgcgttg
    18421 tatgactgtt atatcaccgc cgcgttgctt atcgatatta tgaacacctc cggctggacg
    18481 gcagaacaga tggccgatat caccggacgt ccgtcgttga tgacgacctt cacctttggc
    18541 aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta tctgcgctgg
    18601 ttatttaaca acctggacag catgagcccg gagctgcgtt taacactgaa acattatctg
    18661 gaaaatactt aatccgcagg cgtagcgggt aatgttccct gcgccagcgc gaccagaaaa
    18721 gcatattcca tcgctacgcc ttcgtacgat ttaaagcggc cagatttacc gccatggcct
    18781 gagtccatgt cggtacagag caataaaaga tggttatcgg ttttcagctc gcgcaattta
    18841 gcgacccatt ttgccggttc ccaatattgc acctgagaat cgtgtaaacc ggtcgttacc
    18901 agtaaatgcg gataagcctg tgcggtgacg ttgtcatacg ggctgtagct tttcatgtac
    18961 tcgtaatatt gcggatcctg cgggttaccc cactcttcaa actcaccagt ggtaagagga
    19021 attgattcat caagcatcgt tgttacaaca tcaacaaacg gtacctgggc gataacgccg
    19081 tggaataact ccgggcgttg attaattgca acgcccatca acatgccccc cgcactcccg
    19141 cccatcgcat aacaaagcga aggagagcca tagcccagtt ttaacaatgc atcgcaggca
    19201 tcaagataat cattaaacgt atttttcttc ttcagaaatt ttccgtcttc gtaccattgt
    19261 tgccccagct caccaccgcc gcgaacatgg acaatggcgt agacaaagcc acgatctaac
    19321 aaactcaagc ggctaaaact gaaatcggca tcaatacttg cgccgtaaga accatagcca
    19381 tacaccagca acgggttgtg tcctttgcga aaatgtttgc gatggtagac caacgaaacc
    19441 ggaacttcga cgccatcacg ggcgactatc cacagatgtt cactgcggta attcgccgca
    19501 tcaaaaccag gaacttccgt ttgttttaat acacgacgct caccggtatc catatccagt
    19561 tcaaacaaag tgtctggtgt ggtcatggaa gaataaccat aacgcaatcg cgcggtttca
    19621 ggttctggat tgtaggcaat ccaggtcaca taggctggat catcaaaggc aataccaatg
    19681 acttcccggg tcttgcggtt aatttggcgc aaactggtta acccgcgctg acgctcttca
    19741 accaccagcc agtcggtaaa cagcgtaaac ccttccagca tgatgttttc gcgtggcgga
    19801 attaactctt cccactgttg ctcatcacgc atacgggtac ggtataagcc aaagtttttg
    19861 ccgtggcggt tggaacgcag ataaaaacga tgctggtagt gatcaaggct gtattcgtga
    19921 tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg ccatttccgc gtccagcagg
    19981 cgaacttcac tggtggtggc gctggccaaa tgaataacta catagtgctt cgaggtcgtt
    20041 ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt aaatcagttt atcttgcgat
    20101 gctggcgtac cgatggcgtg acgccagacc tgataaggca gcagcgtcac cggatgcttg
    20161 cgaacatagt agaaagtcca ggagtcattt gcccagacaa agctgggttc aacgttatcc
    20221 agcagttccg ggtaccagtt acctgtttcc agattacgaa aacgaatgcc gtactggcgt
    20281 cgggaaagaa aatcttctgc cagcgccatg atggtgttat cgggcgtaat cgccattccg
    20341 cccatcgaat aaaactcact atgagctgcg cgcttattgg catcgagcaa tgtttcccac
    20401 tcatcccact cttcactgaa tgccgattga cgctggtaga tagcatattc acagcctggt
    20461 tcataaatat gccgatagcg gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc
    20521 ggaatgcggt cgatgatttc ctttaagatg cgatcctgca aggcttgttg tgaggccatc
    20581 acccgatgac cgtaactatt ttcttgttgc aggtagtcca ggacttctgg ctgagaacgc
    20641 gtatcgtccc gcagccagta gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg
    20701 tggggaatgc gggcggcttt tggtagcatg ttattgttct ttctggttga aacatcttat
    20761 aagggtggca aaactcaccg gggatgcaag cgaaacaggg tcgtcattgc ttagatgatg
    20821 acaggtaatg gcgcggatat cgaatgttat gcagacagag aaaatcagcc tgttcaaatg
    20881 gctgtgcggt tctggatagc ccgaaatagt caacttcagg ctatccagag agcggaatta
    20941 ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca atgccttcac gaacatcctg
    21001 ggggattttc agcgcgtcac ccagtgcatt caggtaactg cgttccataa aatggtcaat
    21061 atcaatagcc gcgcaactca ggaaatagat ttccagcgcc tcttcttcat tgcggacccc
    21121 ggtagccagg cgttgtggat ccagcggttg ttcgattgcc tgctcaatga gtacacgccc
    21181 ctgcccttcc acgccagctt cacgcaattg ctggtcgatt gccgcacgtt ctttggcatc
    21241 aatatgacca tcacttttag cggcaaagac cagcgcaagg atcaaacgtt ctgtacgctc
    21301 atccagcggc gtactttgcg cgccaaactg cggttcgtcc tgatgcgccg cgcgaatttt
    21361 atctttgtat ttattccaca gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc
    21421 gttggtgcca tatttcgtaa gaagtttacg tgctgattta tttgcgacca gcagcccagc
    21481 cagtccgcct aatgcgcctg gcactaacag tttgcccaat ccctgatccg tagacgagga
    21541 ggtagaagaa ctgctttgcc caagcaggga ttgcagttga tttaaccagt tagccatatt
    21601 tgctcctcag taaccatttt tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt
    21661 atatcgagac aaaagatgcg caaataagcg gggcggtagg ccgttattca aagaaattcg
    21721 cgccaggtaa ggtatttact gtatatcagg tgataagggt attttaggtg aaagtagttt
    21781 gtcgttttga gtggcattgt ttgatgctga ttgcccgatg cgacgctgtt gcgtcttctc
    21841 aggcctacga ctgtggtgcg ttgtggggcg gacaaggcac tcgcgccgca tccgccaacg
    21901 gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt ccgccattcc cgcttcgcag
    21961 tccacgctga cctggtaatg aatatcggca cttttaccac gcacggttag cggtactgac
    22021 catttatcat ctttaccctg aatgtcctgc aaactgaccc aagccacggg atcggcctga
    22081 ccgacaattt tttgatcatc tgcccaacgc gccacgcgat tttgttgata atcacgtttt
    22141 acgctcgcgg caattccggc agcatccaga ccttcacact ttgggaaagt gaccgacttg
    22201 ctggtttcat tattggcagc gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa
    22261 aacgcccctc tttttttcat gtttttctcc atagcacaat gattcaggag aaagcatggt
    22321 acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat
    22381 tcagcaccga cgacctgtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca
    22441 tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact
    22501 ggagaatttg tcagccaggt gtcaagcaga tccgccgtat caagcatttt gtcataagca
    22561 tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat
    22621 ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac agtatatttc
    22681 ttttcggttg agaaatcaac atcagcaata aagacacacg caaacgtttt cgtttatact
    22741 gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg
    22801 gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag
    22861 tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg
    22921 catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg
    22981 gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg
    23041 ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta
    23101 acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag agctgcagct gcccacttcc
    23161 acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgccgc cattggctat
    23221 ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt tattcgctct
    23281 gcagagcaac ttgctcaggc atgggaatac gctcagcaag gcggtcgcgc cggagcgggc
    23341 cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc
    23401 gcggtggatg gcgtccattt ctgtgcacca gtaggtcatc gccaggaaga tggcgactac
    23461 cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt
    23521 gcccgtaaag tggtgctggc actgggcggt tatggtctgt ttggtgtcga gctgtttgtc
    23581 tgtggtgatg aggtgatttt cagtgaggtc tcccctcgtc cacatgatac cgggatggtg
    23641 acgttaattt ctcaagatct ctcagagttt gccctgcatg tacgtgcctt cctcggactt
    23701 cctgttggcg ggatccgtca gtatggtcct gcagcttctg ccgttattct gccacaactg
    23761 accagtcaga atgtcacgtt tgataatgtg cagaatgccg taggcgcaga tttgcagatt
    23821 cgtttatttg gtaagccgga aattgatggc agccgtcgtc tgggtgttgc gctggcgacc
    23881 gctgagagtg ttgttgacgc cattgaacgc gcgaagcacg ccgccggaca ggtaaaagta
    23941 cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atcgtagatc gggcgttttg
    24001 acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat
    24061 cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt
    24121 tcagcgccag gtagtcacgg tagttagccg gagaaatacc acccgtcggg cagaaacgga
    24181 cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta gcttcagccg
    24241 ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc
    24301 tgatccccgg aattagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca
    24361 gacccgggct aattgcgaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat
    24421 tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag
    24481 cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca
    24541 acgcttttgc catcggcact gcgtgttcca gtttttttac cacgataacc ggtacaaccg
    24601 ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc
    24661 gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg
    24721 acagtttttc acgcaaggcg ctgaataatt cacgtcctgt ccccacgcgt gacgcgctca
    24781 ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt
    24841 cgcctgtctg tccattcaca cgaatgatgt ctccgtcgcg cacttttgcc agcagcccgc
    24901 catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga
    24961 gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg
    25021 gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa
    25081 cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt
    25141 caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca gaggttttca
    25201 taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt
    25261 gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca
    25321 gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg
    25381 tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc
    25441 cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca
    25501 gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata ccggccgcgc
    25561 gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa
    25621 cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac
    25681 cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg
    25741 gagaatccgg atgaacaaaa gaagagcctg gcaactgcat ccccataaat tccaccacca
    25801 tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg
    25861 cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac
    25921 gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag
    25981 gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac
    26041 cgaggaacag agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcta
    26101 tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg acaccatcac
    26161 acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc agggctttac
    26221 gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg
    26281 tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc
    26341 aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgacg
    26401 ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga
    26461 ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag
    26521 agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg cctgtaaccg
    26581 gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc
    26641 gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta
    26701 aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa cgaccatcac
    26761 gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg
    26821 catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca
    26881 cttcatcacg acgtacaaac agtgcctgaa taccacgcat ggtttccagc agcagacgtt
    26941 cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga tccagcttgg
    27001 tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc tggatatcca
    27061 cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgatt
    27121 ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg
    27181 gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt
    27241 cgacgcggat cgccacgaag gtttctgtat tgctgctctt gttcgcgccc tcttcttcca
    27301 gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgcccg cgtacggttt
    27361 tttcgcgtac gttggagcgg tcgatgcggc gcagagactt cagtactttc actttttcat
    27421 cgcggatgct gtctgcgctc aggtcagacg gcggagacat cgcaatcatg cagagaattt
    27481 gcagcaggtg gttttggatc atatcgcgca tctgaccggc tttatcaaaa tagccccagc
    27541 gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt
    27601 cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt
    27661 ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct
    27721 gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc
    27781 gtgccggttt agcattcagt ttagcctcgc caagcccttt gcaaattgcg ccaaaagtgc
    27841 tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga
    27901 gacggctgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca
    27961 gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga
    28021 caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt
    28081 ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg
    28141 caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg
    28201 ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata cttgtaattt
    28261 tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc
    28321 gtgcgattac ggtaaaagcg cgcaaagtgc ggcaaaactg ataaaaaatc atcgttttcc
    28381 attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc
    28441 ttttgtcatt aacacagcac acaggcgtaa tatctgacaa aactacattt cgatttcttt
    28501 cagtgcggaa atcgtcatta cccgtgagtt tctttacatc atgaatatgc tggaaaaaat
    28561 ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc
    28621 ttcgcccgat aacgcgatcc attcgagtat tgctgctctg gcgctggaag ccaatgttag
    28681 cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa
    28741 acttcatctg gcacaaagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga
    28801 agatgacagc gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga
    28861 tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca
    28921 ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat
    28981 gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg
    29041 catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag
    29101 aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa tggtgattgc
    29161 cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt
    29221 accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat
    29281 agatgtgctg gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa
    29341 gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga
    29401 cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt
    29461 tatcgccatc gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc
    29521 agtcaacgga gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag
    29581 gcccagcaac agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg
    29641 tacgtatgaa cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc
    29701 gtgagattgc cgcaagactg gggcgtcatg tggctattct gggtgacctc caggggccca
    29761 aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc
    29821 tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca
    29881 aaggcctgcc tgctgacgtt gtgcctggcg acatcctgct gctggacgat ggtcgcgttc
    29941 agttaaaagt actggaagtt cagggcatga aagtgttcac cgaagtgacc gtcggtggtc
    30001 ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga
    30061 ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg
    30121 tctccttccc acgctgcggc gaagatctga actatgcccg tcgcctggca cgcgatgcag
    30181 gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg
    30241 caatggatga cattattctc gcctctgacg tagtaatggt tgcacgcggc gacctcggtg
    30301 tggaaattgg cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc
    30361 agctaaaccg agcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga
    30421 tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg
    30481 tgatgctgtc tgcagaaact gccgctgggc agtatccgtc agaaactgtt gcagccatgg
    30541 cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg
    30601 acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc
    30661 acctgaaagg cgttacggcg atcattacca tgaccgaatc gggtcgtacc gcgctgatga
    30721 cctcccgtat cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga
    30781 acctgactgc tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg
    30841 tagcagctgc cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg
    30901 acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc
    30961 gtattttaac ggtagagtaa tctcacctgc cccggccgct gatgaccggg gtatttagca
    31021 acacataaat taggactact cattcaatcc aatcataatc acagccccct gtaaaatgaa
    31081 tacagggata gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct
    31141 gtgcaacact atatccaata actgccatcg gtaagccact atctttggag tattgtgtct
    31201 gcacccatgt tttaattgaa tc
//
LOCUS       DAZFAH010000034        30813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFAH010000034 DAZFAH010000000
VERSION     DAZFAH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.034"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHJWB_001467"
     CDS             complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHJWB_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO4313121.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(581..799)
                     /gene="yiiE"
                     /locus_tag="ACHJWB_001468"
     CDS             complement(581..799)
                     /gene="yiiE"
                     /locus_tag="ACHJWB_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4313122.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1404..1694)
                     /gene="nadS"
                     /locus_tag="ACHJWB_001469"
     CDS             complement(1404..1694)
                     /gene="nadS"
                     /locus_tag="ACHJWB_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4313123.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1695..2006)
                     /locus_tag="ACHJWB_001470"
     CDS             complement(1695..2006)
                     /locus_tag="ACHJWB_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4313124.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2235..3143
                     /locus_tag="ACHJWB_001471"
     CDS             2235..3143
                     /locus_tag="ACHJWB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4313125.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3207..4148)
                     /gene="fabY"
                     /locus_tag="ACHJWB_001472"
                     /gene_synonym="yiiD"
     CDS             complement(3207..4148)
                     /gene="fabY"
                     /locus_tag="ACHJWB_001472"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4313126.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4193..4630)
                     /gene="dtd"
                     /locus_tag="ACHJWB_001473"
     CDS             complement(4193..4630)
                     /gene="dtd"
                     /locus_tag="ACHJWB_001473"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4313127.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4627..5499)
                     /gene="yihY"
                     /locus_tag="ACHJWB_001474"
     CDS             complement(4627..5499)
                     /gene="yihY"
                     /locus_tag="ACHJWB_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4313128.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5493..6092)
                     /gene="yihX"
                     /locus_tag="ACHJWB_001475"
     CDS             complement(5493..6092)
                     /gene="yihX"
                     /locus_tag="ACHJWB_001475"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4313129.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6233..7120)
                     /locus_tag="ACHJWB_001476"
     CDS             complement(6233..7120)
                     /locus_tag="ACHJWB_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4313130.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(7110..8459)
                     /locus_tag="ACHJWB_001477"
     CDS             complement(7110..8459)
                     /locus_tag="ACHJWB_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313131.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(8558..9991)
                     /locus_tag="ACHJWB_001478"
     CDS             complement(8558..9991)
                     /locus_tag="ACHJWB_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313132.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(10061..11071)
                     /locus_tag="ACHJWB_001479"
     CDS             complement(10061..11071)
                     /locus_tag="ACHJWB_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO4313133.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQSGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(11104..11991)
                     /locus_tag="ACHJWB_001480"
     CDS             complement(11104..11991)
                     /locus_tag="ACHJWB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO4313134.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(12016..12894)
                     /gene="yihU"
                     /locus_tag="ACHJWB_001481"
     CDS             complement(12016..12894)
                     /gene="yihU"
                     /locus_tag="ACHJWB_001481"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4313135.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            13067..13963
                     /locus_tag="ACHJWB_001482"
     CDS             13067..13963
                     /locus_tag="ACHJWB_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4313136.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            14003..14791
                     /locus_tag="ACHJWB_001483"
     CDS             14003..14791
                     /locus_tag="ACHJWB_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4313137.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14897..16720)
                     /gene="typA"
                     /locus_tag="ACHJWB_001484"
     CDS             complement(14897..16720)
                     /gene="typA"
                     /locus_tag="ACHJWB_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4313138.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            17093..18502
                     /gene="glnA"
                     /locus_tag="ACHJWB_001485"
     CDS             17093..18502
                     /gene="glnA"
                     /locus_tag="ACHJWB_001485"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4313139.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18676..19725
                     /gene="glnL"
                     /locus_tag="ACHJWB_001486"
     CDS             18676..19725
                     /gene="glnL"
                     /locus_tag="ACHJWB_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4313140.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19737..21146
                     /gene="glnG"
                     /locus_tag="ACHJWB_001487"
     CDS             19737..21146
                     /gene="glnG"
                     /locus_tag="ACHJWB_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4313141.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            21258..21368
                     /gene="yshB"
                     /locus_tag="ACHJWB_001488"
     CDS             21258..21368
                     /gene="yshB"
                     /locus_tag="ACHJWB_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4313142.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(21558..22931)
                     /gene="hemN"
                     /locus_tag="ACHJWB_001489"
     CDS             complement(21558..22931)
                     /gene="hemN"
                     /locus_tag="ACHJWB_001489"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4313143.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(23120..23629)
                     /gene="yihI"
                     /locus_tag="ACHJWB_001490"
     CDS             complement(23120..23629)
                     /gene="yihI"
                     /locus_tag="ACHJWB_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4313144.1"
                     /translation="MKPSSSNSRSKGHAKVRRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(23748..23914)
                     /locus_tag="ACHJWB_001491"
                     /pseudo
     CDS             complement(23748..23914)
                     /locus_tag="ACHJWB_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24211..24843
                     /gene="yihA"
                     /locus_tag="ACHJWB_001492"
     CDS             24211..24843
                     /gene="yihA"
                     /locus_tag="ACHJWB_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4313145.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(25225..28011)
                     /gene="polA"
                     /locus_tag="ACHJWB_001493"
     CDS             complement(25225..28011)
                     /gene="polA"
                     /locus_tag="ACHJWB_001493"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4313146.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            28375..29307
                     /gene="yihG"
                     /locus_tag="ACHJWB_001494"
     CDS             28375..29307
                     /gene="yihG"
                     /locus_tag="ACHJWB_001494"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO4313147.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(29348..30778)
                     /gene="yihF"
                     /locus_tag="ACHJWB_001495"
     CDS             complement(29348..30778)
                     /gene="yihF"
                     /locus_tag="ACHJWB_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4313148.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         7702 a         7928 c         7384 g         7799 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaatcg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg agggcgtttt ccagatacga
      481 tgtaacagtt gcagttatta taagttcaag taaaacagcc ttgtattaat accagaataa
      541 gggcatctgt ttttttatat tcaagaatga aaaatttttt tcattcctta tgctccttac
      601 actgatcatc aacaccaact acctccccct tttgcctgcc tccctttgct ttacgcaccg
      661 gaaattgctg ccgctccaga tattgctcaa ccgcttcgcg taaaattgca gatcgtgaaa
      721 ccttacgcca gccccgaagt ttgttcagcc gtttaattgc ctcttctgat aaatgaagaa
      781 aaaccgtatt catcgccatt accaccacct tacctatatt actcattaat gtatgtgcga
      841 aaattacgcc ggaaaatcat ttaaaaaaag cggatttttg catgttgttt tgtttttgcg
      901 aggcgttttc cagaaataat aagagtgttg cagttgtttc atttattact caaaaatggt
      961 ttagttatcg cccaggatga gttatggatg ctatgaatat attatattca gggatgttta
     1021 ttttttgatt ggttctttat ttgcataatc tgaatggtgt acccgaaaaa tgtcagcatc
     1081 ctaacagcac ccggatttaa ggtgaaatta atcatccatc acagaccggt cagtactgcc
     1141 accaaccggt ctttacttgt tcatcgttat aattttgttg gtaacagttc gggaaacaag
     1201 gaattccttc cggaaccccc cggtatcatc cgatagcatt gacgtcaatc atcagcgcga
     1261 cgatgagttt ttttaatcgc atcatagggt ggcgacgttc agcacgatta cgggtcatag
     1321 caaaactcct gggcaggcga ccactatgcc gctttcacag gagttctgaa tgctagtgct
     1381 ttctgtatgg cataaccatg cctctaacgg ttaagtgctt gcagcacatg ttgcggatca
     1441 ttggcaatcg ctcgcagcaa agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc
     1501 cagttacgca acgtgtctac gcttacagaa ataagatcgg caaatttaga ttgtgacaac
     1561 cctgaagcgc ggcgtatttc tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc
     1621 tgtcgcgttc cttcaataat ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa
     1681 agtgccttat ccatttacca cctcgtattt aacaaccgga gcattgcttt ttcctgggga
     1741 gacagatcat ctttaatacc cttacgataa ataagcagta atctgatctc ataatgttta
     1801 acctgatgaa agtaaataac cctgacacca gcacgtttcc cctttcctcc tgacacccat
     1861 cgaacctttc ttaacccacc agtttcaggt attacctcac catagtcagg attcaaggcc
     1921 aggaaaaact gaaaacgact gaattcatcg tcggtcagca gtttttgaac atcttcagta
     1981 aaaatttccg tttcgatgaa gagcataatt atacgccatt ggcttagagt aacaatcctt
     2041 tttgtacgtc attgacgtac ccttgaaacc actagcttac tgatagtgct ccagcaaaac
     2101 cacgtctctg ccgggtttgt ctttagattt gcgaggcgct ttccagattt ttcacgcccg
     2161 tgaatttccc tgtatccatc cagcaaaaac gggtgtataa gactgtacat tcatacagca
     2221 aaataagttc tactatggca ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg
     2281 ctgcgggtcg cccctcatcg cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg
     2341 ccagcgctgt ccttgcgggt gagacagaaa cgcgggtaga tattcagact atcgaacttg
     2401 aagggatgac cttacgaata gtgtcgccgc tgaatgcccc gacattactc ccgacgataa
     2461 tttattacta cggtggctgt tttgttagcg gcgggttcgc aactcacgac aaccagctgc
     2521 gccagttagc ctattacggg caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg
     2581 aacatacttt ccccgccgcc catgatgatg cgcaacgagg tgcagaaata gtctggcaac
     2641 acgccgaacg actcggcgtt gataaacaac gtataactct tgccggtgac agtgcgggcg
     2701 gacatctggc gctggtaact gctttgcgat taaagcgtgc tggcgagtgg caaccggcac
     2761 aactcatctt aatttatcct atgcttgatg cgactgcgca tttcgaaagc tacatacgca
     2821 atggtcacga ttacatcatc acacgcgaca ctttactgag cggctttgag atgtatctgc
     2881 caggcattga acgcaggcat cctgaagcca gccctctttg gcgcgatgat ttcaatggct
     2941 tgccacctgt acatatcatt accgctgaat acgacccact ttgcgatgaa ggtgaagcgt
     3001 tgtatcaccg catgacaggg caaggagtgc aatgcacctg ccaacgctac cttggcgtta
     3061 tccacggatt ttttcagtta ggtggtatca gcgaggccgc gcgatcggca ctacgggatg
     3121 ttgcctggag agcgggaagg taacttcgga cctgggccct gaaaaggttt tcagattgaa
     3181 aaccttttca gtcaagtaaa acgcccctac tcttcttcgt tcccgccctc ttcatacggg
     3241 ccaaatggct tcgcgggcag aacgatatac gtgccttcaa acactgcacc cggcgtctcg
     3301 tcgccaaaga tttcaacttg catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg
     3361 agatcgccgc ttaaggcacc aaggtcggct accgcatgag gtttaccgct aatcggcttg
     3421 ctgtagcgga tatgcgcatc cgccagaata atcgttccgc cgaggtggcg ttcacgcagc
     3481 atcagccaga taagtcccca accggtgagc gtcgccagcg agaataaact cccggcaaac
     3541 aacgtatggt gcggattctg attaccggtt tctggcatgg tggtgataaa tttttgcccg
     3601 gtatattgtt gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc
     3661 tgttgcagct gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcaacgggt
     3721 ttaatcatca aaaaatggcg aatcggcgtg gtagttgggg tggtgatctc cccctgatta
     3781 atgaatccca gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc
     3841 ttaacgcctt cctgacgcgc caccgactcc agggtcatcg ccatcagcgt gcctaaccct
     3901 ttgtcctgca cgtccgggtg aacggccata aagcgaatgg acgcttcatt gtcggcatta
     3961 atatacagtc ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc
     4021 gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac
     4081 atttcccagc gaaactgata gtaacgctct aactcttctt ctgtttgtgg aacccgaagg
     4141 tgatacatag cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct
     4201 gcaaccagaa tgttacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc
     4261 gtcctgtttg cgtgttcatc tcttgctgac ggcagcgttc gacgaaatag tcatataacg
     4321 cctctgcgcg atccggtgat gcgcctttgg agaaacttgg gcgcatcccc cgttcggtat
     4381 ctgcggcgag ggtaaactgg gaaaccacca gcacactgcc gcccgcctgt tgcacgttga
     4441 ggttcatctt gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac
     4501 ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc
     4561 caatttcgcc cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta
     4621 atgcaatcat ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc
     4681 cccgagagtg acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca
     4741 aacaaagaga atggggatca ccgccaacac gccgtaaatg agctgatatg acgggaacat
     4801 ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac aggagcgcgg cgacaaacgc
     4861 gccgacaatc gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca
     4921 gaaggagatc cacgacaaca gcagcggaaa aatacgcagc acgttatcga tgacagtatt
     4981 gagatcgctc gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc
     5041 cagcagcggc cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg
     5101 cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcaacaataa
     5161 cgcggtgacg atcagcccgc acgccccaac ggcggtcatc ttgttggaat tggcaacaaa
     5221 ttgttcgata taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa
     5281 gtgacgtaac tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac
     5341 aacggcaacc agcggcacta atgagagcaa agacacatag gcaaggttac ctgccagggt
     5401 tgtcatgttg tcctcatcaa tacgttgcca gagtagtttt agccaggccc atagtggacg
     5461 ggtacggtgc ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc
     5521 gggatggtgg ttttatcttt caccagaata ctggtaatgc ccagctgatt ggctccttct
     5581 atattatcgg cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc
     5641 aaaacatgct ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag
     5701 atatggtcag cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg
     5761 cggttggtat tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg
     5821 gcgatcactt ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg
     5881 tagcttagcg gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt
     5941 tccccacgct catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc
     6001 agcggaatac gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca
     6061 atcacattac ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat
     6121 taactgtagc gggaaaggat gggtttggct atgtggcaat aagggtaatc ttgtgatgct
     6181 gattgcgaaa aagcagagta atttcactgg ctttttcgca atagatgaat ggttaagatt
     6241 caggtgtaaa ttgccaacga gtttcgatga ccagcggctt atctggtgtc acaaattccg
     6301 gcgttagatt tattgcgtta ggtgcctgag tcagcgggtt aatacaggtg gcatcgggct
     6361 gtttatcaaa taccaccaga ctgtttgcag aagagatcat ggtcatcgcg agtttaccag
     6421 gccacagtaa cttaaccttc accccgttat aaaaaccaaa acagtcatcc cagggaccgg
     6481 cttgaggaga gatacgattt ccagttggca gttcatcact acccgcttct tcttgccagt
     6541 ccgcatcaaa aagcacctgc aacgattctg tattttgcgg agtcagcttt ttcgcaaacc
     6601 acggatgcca gcctgcggag gccgggaatg tatcggcata tgaatggact tccagttgca
     6661 aaaccagcgc atcgttctcc agcaaaaaag tctgaatcac ttccccttgc cagggccacg
     6721 gtgaagccag gggcatacgt aaagtcaggg aatctgctgt tttgtcgatg atttcccacg
     6781 ttgagtaaca agccatacca tgcaacgcat gtggtggttt attggcgggt aaagaataac
     6841 attgcccacc agcattcagc gtcgcattcc ccagtcgccc tgcccacggc accattggga
     6901 aacagccata ctgaaaagct ctgcgctgcg gctgccattg gcgtaatact tcagaaccaa
     6961 atgcctttaa cgaggttatc ctagcaccat catgcggata aatctgcagg tgaatattgc
     7021 ctttttccag cgtcactaac tctcctacag gcccaggcca tggcgcgtca aacgcctgtt
     7081 gtagcgccag gtaatgctcg ttttttcgtt cagttttcat gagattgttc cctgatgtag
     7141 cggtaagaag cggtttcttg ttcatcggtc ggctcaatca gccagacgca aacaaaggaa
     7201 agaactgaga cagcaaataa aaacagagcg acataccacg gttttcctcc ccctagcgcc
     7261 agcaaacttg aggcgataag ggggcttagt ccgccaagaa tcgcggaaaa ttgataagca
     7321 aaagaaaggc cggtatatcg tactttggta ccgaacatgc gggtgaaaag tgttggctgc
     7381 acggcaaaca tcatcgtcgg acctaagttg tagccgagga tcatactgca ccagatgatg
     7441 agcgtacttt tgctatccag taataagaag aaaggaaacg cgaacaggac acaaaatcct
     7501 gctcccgaca aatacagact tttttgacct atacgatctg ataaccatcc aaccaatgga
     7561 caactgaaaa taccgatcgc cgaggcaatc aacatacccg tcaggggaat atctcgcgac
     7621 aatgctaatt ggctggaaat atagacaata ccaaaggcgt taataatatt agaggagaca
     7681 ctttccgcca gcctcgcacc cagtgccaat aaaatatttc gtggatgttt tttgaataat
     7741 tcaatcaacg gaggaacgga cttttcttta ttattcgtcg ttttttgctt ttgaaaatcc
     7801 agtgtttctt cggtatgcag gcggataaac agcccaacga tcaacatcac cgcagaaagc
     7861 caaaacggaa tgcgccatcc ccaggagaga aactgctccg aagtaagaaa atggttacag
     7921 agagcaaaaa taccggttgc cagcataata cccacagacg cagccgtttg cgggagtgaa
     7981 cctaaaaatc cgcgtcggga ttcgggcgct gattctatgg tcatcaatgc cgctccgccg
     8041 tactcgccac caagaccaaa gccctgaatc aaacgcagca ccattaatac aagcggtgcc
     8101 caaatgccaa tttcctgata cgtcggaata aaaccaatca aaaatgtgga gcaaccgaca
     8161 atagccagcg tccagataag tgttattttt cgcccgattt tatcgccaaa atgaccaaag
     8221 acgatgccac ctaaaggacg agtcagaaaa ccgacaccaa aggtcgcaaa ggcggctata
     8281 agacctataa aaggatcgct gtctggataa aacagcggtg caaataccag tcctgctgcc
     8341 gtgccaaaaa taaagaagtc ataccattcc attaacgcgc ctgcgaaact gcccataacg
     8401 acacgtctca tggctttacc ggaaagccct tcacgcggtg cgggtaactt ttcagacata
     8461 attttcctct cctagtaggc gagaaatctg ggatacgaac cactaaacgt gggcagtttg
     8521 aattaatgcg catcggttta agccgatgcg cattaattta tttcttcaat tcagccagaa
     8581 cctctgcatc ctcagcatca acatttgcac ccagtggttc ccaattaatc aatacgcagg
     8641 tcaggaaccc caggaatggc acgattgcca accagtaaaa cgcttcagtt ttcaacgcac
     8701 cccacagcat cggccagaaa atgagtcctg caattgcccc tgtgcgcata atagcgcggg
     8761 caaatccgac cccagtaggg cgaatagcgg gaggataaga gagtgtggca atggtcatgc
     8821 ccagcccacc aggacccgct gaatggaaga aaataattga tcccaataac cctaaagaca
     8881 gccagggatt agtcgttgcc agtgcgccta acgacagcaa tgctaaggtc acaacagcaa
     8941 atccatacat cgactgtcgg cgagtaccca gtcgttggag aataaacgag ccgatcatcc
     9001 cgcctgtcac cccacaaagg gcattcacga cggcagaacc ggtcaacgcg ccagtaagcc
     9061 cgccacttat tattcccgcc aacgtaagag gaagataaac acctacggcg ttatattgcc
     9121 acgcctgcat ggttgccacg acacagccaa gaatggtgcg tttgcggtaa cgatcattaa
     9181 ataagatgcc atatccacca ctgtatttat tctctgcttt ttccgagaat ttcgcttctg
     9241 ttgtacccga tgccgcaacg cgagcctgaa caccataacg ttttcccaga atgtcgcacg
     9301 cttcctggta acgcccgacg cgtgcggccc acattgcgga ttcaccaata aagaagtaac
     9361 gcaaaatcat gaagatgact gcaaaaatag ctccgatgaa tattccgtaa cgccacaatt
     9421 gttcttcagc gatacccgac agaagcagcg gcaaaagtac caaataaaca acggttgtcg
     9481 aaacatacca ggccatttgc cagaggttga ccgacgttcc ggttttcttc gacgttgaac
     9541 cacgaagctc tgccacggca ttggttgcca aagggaagtc gatgcctaaa ccaaagccca
     9601 tgatgacgcg gcacaccagc acccaccaga tactgggagc aatagctacc gctgcagcac
     9661 cgatggtgca cagccccatg ccaataataa atgcccgctt ttgcccaaag cggtttatta
     9721 ctcggttcgc taataaaccg ccaattaacg cggcgattaa tactgatgca ttgacggttg
     9781 cagcaagacc cggcgaaatg ccgaattgag cggcaatgta tttattacca aaccccacca
     9841 tcgccgcctg gtaggcatca atcaatatgc cgcccaaagc aataaaaata acgccgatac
     9901 tggtgttgat tgcagagttc ttattaacaa tatcaattac gtcctgtggc cgactaattg
     9961 aataagttgc aggactggtg atttgactca tcgcagtgtc ctttttaatt gttgtagggt
    10021 ggcgctgacg tgacgcagcg ccagacagag atttagaaaa tcattccggt gcaaattgca
    10081 gcataacctt tatggagtca ttttgctgcg cgcgttcaaa tgccgccgct gcatcctcaa
    10141 tggcaaataa atcggtaacc agttcactgg tatcaaaccg cccatccgcc agccattcga
    10201 taacagcatc gaaatcttca gggatgtaca tgccagagtt gagcaagtca cgctcgaaac
    10261 gttgcatcat cggtaatggt atttcacgcg gcccacttgg tacgcccatg cacacgacgg
    10321 tgcctccggc atatacacgg gtacatgcat cgttaagcgt ggcctgcgct gcaacgacat
    10381 caatcgcccc cgtaaatcgc acatctgcgg ccagctcacc tggtgcccaa acttctgctg
    10441 cgcccagctt taacgccagc gcgcgtttgg cagcgtcagg ttcaatcacg gtaatcttac
    10501 ctgcccccat aatgcgcagt gcctgcacga tggataagcc tatcgttccg gcaccaatta
    10561 ccagtacgct ttccagagaa gctttcggca ttcggttaac gcagtgacgg gcacaagccg
    10621 ccggttcggc aaaggccaac acttttagcg gtaaagaggc tggtgcgaca tggcaattac
    10681 gcgcaggaac aatgtgttgt gagcgagcaa agcccggtgc gcgaaaacca gcaacctgcg
    10741 gtggttcaca aagattaaaa cgtcctgctt tgcaggctcg gcattccatg caagccatga
    10801 tgggatcgac cacaacatga tcgcccgact gtacattttt aacgtcgctg ccaacttccg
    10861 taacgcgcgc tgcaatttca tgaccgggga cgacaggtgg tttggcaaac ggatgaccgc
    10921 ctttcagaac atgcagatcc gaaccgcata tcccatagaa cacaggcgca atccgcactt
    10981 cgtgcggttt aagttcctgg tgtggtactt caacatattg agtggtgact ttcccaatat
    11041 ctgaaaataa aacctgggta attttatcca ttgttattct ccagaatcga tatccatagg
    11101 ggattagcgt tgcgcgatac cttcatcgac aatttctgcc aaccgttgta aacgaggaac
    11161 agagacatcg cgcagcatag tttgtggatc ttgtgcacca acaaccgacg cccaaaccgc
    11221 tcgtccagcc aggaagcccg acgcaccacc tttcatggca atacttactg cacggccaaa
    11281 aatatcggca tcaacgccag aagaaagaat tacccacggc atcttcatct gatcgttaag
    11341 ttgctgacag gcggcaagta aggttttttc atcgcctttg ccccctaatg gcatctcggc
    11401 tttgtaaaga tcagcctctg ttccaccaag ctctgcggcg gcggcaacaa tggcgctttc
    11461 acgatcaaag tcccaaccgc gacgaggtgg acgcaccaca ggttcaataa tgctcaccag
    11521 cccggcacta cggcagcgac gaacaaattt atcaaccatt gccagacgtt cctctgcggg
    11581 ttcatcttca cgccagagta caagtaattt cattgcctta gcccccattt cacgggcttt
    11641 atgcgggtcg acggacatat ctatttcaac gctatcaacc gggatgccat tacctgggat
    11701 aaaaagatcg gcagcgacaa tcaggccgca agaattagca acagcgccct gctcgactat
    11761 ttgctcaaga caaaattgtt tatccgccag aacggcagaa gcataaggag aaaggattcg
    11821 ggtcgcggca actttaaaat cggtgagtac actgtcggcg atgggttttg gctgccctgc
    11881 tgccgcaaac atcaagcgca tggcctcacg ctgatcgaca gctaacatgg caaatccacc
    11941 acctgggcgg gagatatctt tcagcgtaaa tggggtgaag tgggatgaca tagaaactcc
    12001 tgttaataga taaaattaaa gaccagcctg ctggcgaacc tgatttagca gggcactcca
    12061 gtcctgtcgc cctttgccgt cttgacttgc tgcagcgtaa taactttctg ctgcaatccc
    12121 catattgagc ggtgcccccg tctcccgagc gacgtcaact gcaatacgca gatctttcaa
    12181 cgccagatcc accatgaagg cgggtgaaag atcgcctttc agcactttgc caggccaggt
    12241 ggtcgtaaaa tgtcctttcc ctgctgccgt accactcatc accttaattg cgacatcaag
    12301 atttaatccc agtgactcac ataacacagc ggcctcggag gacagcgcat taagcgcaat
    12361 gctcatatag ttgttaatta gcttcacgcg gatccccatt cctgggccgc cagcctcgac
    12421 cagctcatta cccatgcaca tgagaatcgg gcgtgcacgt gcaatctgct catcagtgcc
    12481 acccgctaaa atcagcaatg tgccatcaat cgcattcacg gacgttctgc ccacgggagc
    12541 gtccatcata ctgatgcctt tttcttgtaa ctcccgtatt aatgcatcgg tctgtaaggg
    12601 atgaatagtc gacatatcaa tcaataacgc gtcagatgaa acagcctcac agacgccttt
    12661 ttcccccaac agaacctgtc gaacaatgtc accattggga agcatagtga tgataaattc
    12721 agcgcctgct gctgcttccg ctggggtttg tgccgcagtg gccccctgcg taactagcgc
    12781 atcgacggcc tgtgcattga catcaaatac ctgcaatgaa tgcccttttt gcaatagatt
    12841 gttagccatt gggctaccca tctgccccag cccgagaaat gcaatggttg ccatgaatac
    12901 tctcctgtct gacattgaca aaataatgac aattgaaaat tcgttttcgt ctgtttttga
    12961 gcatatttgt aatttatgtt caaaaacacg acacgcatca ctaatttgat catttgcgtc
    13021 atttaaattg accaaagacg acaaaattaa acaggacaag acaaatatga ccagaattgc
    13081 atgcgtgggt atcacagttc aggacagaat ttattctttg cccactctgc cggagggcgg
    13141 cgggaaatat caggcaaatc attatcttga gattggtggc ggcccggcag caacagccgc
    13201 tgttgcaatc gcaaagcttg gcgtggaggt tgatttcatc ggtcgcgtcg gcgatgacag
    13261 ttgtggaaat accttacttg cagaacttga gggttggggc gtcaataccg cattttgccg
    13321 ccgatatcct aacgcccgtt cgtcgcaatc agcgatcctc gttgatcagc atggcgaacg
    13381 gattatcgtt aattatccaa gtcctgatct cggtactgat gccgaatggt tagaagcgat
    13441 cgatttctct cgttatgatc tgattctggc cgatgttcgc tggcatagcg gtactgaaaa
    13501 agcattctcg ctagcgcgcc ttgcgggtgt gacaacgctt ttagacgccg atatgacacc
    13561 gcaggatatt tctccacttg tcgcgcttgc cgatcacgcc gtgttctcaa caccagggct
    13621 gaaacgcatg actggcctgc agtcacctga agaaggtttg ttccaggcaa cgacacaaac
    13681 agcaggtaaa gtgtatgtta cgctgggttc tgaaggatcg ttgtggattg aagatggtca
    13741 tttgtgtcaa caggaggcat tttctgtcaa tgtggttgat accactggag caggcgatgt
    13801 ttttcacggc gcactcgcgg tagcgttggc agaaaaaatg ccaacaaaag aggctattcg
    13861 ttttgccagt gcggttgccg cgatgaaatg tacccagcct ggcggtcggg ccggcattcc
    13921 taatcgtgag caaaccgaat catttttgtc actttatgcg taaaatggta aacatctacc
    13981 gaggaggggc aagggagtat tcatgagtat catcgaagtg acaggtaatc cacgccatga
    14041 ccaactcgtt catctaatcg cagaacgcgg ttatatgaat attgaagaac tggcacaact
    14101 gctggatgtt tcaacgcaaa ccgtgcgccg ggatatccga aaactcagtg agcagggctt
    14161 gatcacccgt catcatggag gggccgggcg cgtctccagc gtcatgaata ccgcttttga
    14221 gcaacgggaa ctttcgctca ccgccgagaa acgggcgatc gctgaggcag tcgccgatta
    14281 ccttcccgaa cgctgtaccg tctttatcac catcggtaca accgtagaag ccgttgccag
    14341 ggcattactc aaccggcgtg atttacgcat tatcactaac agcctacgtg tggcacagat
    14401 tctttataag aatcaggata ttgaagtgat ggtgccggga ggcactttac gcgctcataa
    14461 cggcgggatt atcggcccag gagccgtgga ttttattgaa ggcttccgcg cagattattt
    14521 aatcaccagt ataggtgcca ttgaacatga cggcacccta ctggaatttg atgtaaatga
    14581 agcgttagtc gcgagaacga tgattaaaca tgcgcggaat acattgttag tggccgatca
    14641 tacgaagttt gccgcgtctg ctgccgtttc aattggcaat gcacggaatg tcagggcttt
    14701 ttttactgat gccccgcctc ccaattcttt ctgccagttg ttaagtgaag agaatgttga
    14761 actggtggtt gccgagcaag aagtatcctg aaattgaaat ttcggaaaat ccgctgggaa
    14821 tttcagcgga ttttgttttc cataagcaaa aaaaacctgc catcgctggc aggtttttta
    14881 tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcgacga cgatcgtttt
    14941 ccgtcaggtg acgtttacga atacggatag aggtcggggt cacttctacc agttcgtcat
    15001 catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc agaacaacgg
    15061 cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg cagtttacag
    15121 tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact tctgcaccgt
    15181 gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg accgctttac
    15241 cctgaccgtt agagatcagt acgccgttct gacgctgacc cacttcaccc ggacgtacgt
    15301 cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg gtcatgaact
    15361 cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt acgcggcctt
    15421 taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc tgcattacag
    15481 aaccctgatg ctgttcttca acgtccagag tcacgttttc atatggctct tgtttacgac
    15541 cgtcgatttc acggaagata actttcggac gggataccgc cagttcgaaa ccttcacgac
    15601 gcatgttttc gatcagaaca gacaggtgta gttcgccacg accagaaacg cggaacgcat
    15661 cagcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct ttgttcagac
    15721 gatccaggat ctgacgagac gttacgaact taccttcttt accgcagaac ggcgaggtgt
    15781 taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc agcgcttcaa
    15841 cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gccaaggccc gtgatcgcaa
    15901 cgatatcgcc agcttccgcc aggtcggttt cgatacgttc cagaccgagg tggcccagca
    15961 ctttaccgac tttcgcgttg cgggttttgc cttcgctatc gatgatagtg acctgctggt
    16021 tcggcttcac tttaccgcgc ttgatgcggc caatgccgat aacgccaaca tagctgttgt
    16081 aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc ggcgcaggaa
    16141 cgtggtcaac aatcgcctgg tacagcgggg tcatgtcttc cgccatatct tcgtggtcca
    16201 gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc tgctcgtcgg
    16261 tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca ggacgcgcgc
    16321 cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca aacgcttttt
    16381 tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc agcagcactg
    16441 agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc cccggggtat
    16501 caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg aggatggtaa
    16561 tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt tcggcacgag
    16621 agtcgaacgt accggattgt tggagcagct tgtctaccag ggtggtttta ccatggtcta
    16681 cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct caggcattag
    16741 aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac aaacatcctc
    16801 cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa aaagggttat
    16861 ccaaaggtca ttgcaccaac atggtgctta atgtttccat tgaagcacta tattggtgca
    16921 acattcacat cgtggtgcag cccttttgca cgatggtgcg catgataacg ccttttaggg
    16981 gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac ggccaaaata
    17041 attgcagatt tcgttaccac gacgaccatg accaatccag gagagttaaa gtatgtccgc
    17101 tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt tgcgcttcac
    17161 cgatactaaa ggtaaagaac agcacgtcac tatccctgct catcaggtga atgctgaatt
    17221 cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag gcattaacga
    17281 gtccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt tcttcgccga
    17341 ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag gctatgaccg
    17401 tgacccgcgc tccattgcga agcgcgccga agattacctg cgttccactg gcattgccga
    17461 caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc gtttcggatc
    17521 atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga actcctccac
    17581 ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt acttcccggt
    17641 tccaccggta gactcggctc aggatattcg ttctgaaatg tgtctggtga tggaacagat
    17701 gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga acgaagtggc
    17761 tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca aatatgttgt
    17821 gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac cgatgttcgg
    17881 tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg ttaacctgtt
    17941 cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg gcggcgtaat
    18001 caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt ataagcgtct
    18061 ggtcccgggc tatgaagcac cggtaatgct ggcttactct gcgcgtaacc gttctgcgtc
    18121 tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac gtttcccgga
    18181 tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc ttgatggtat
    18241 caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc tgccgccaga
    18301 agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga acgaactgga
    18361 tctggaccgc gagttcctga aagccggtgg cgtgttcact gacgaagcaa ttgatgcgta
    18421 catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc cggtagagtt
    18481 tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaac aacgccggga
    18541 tttagttgcc gtggaaactt tcagcccatc tctgcatggg cttttttctc cgtcaattct
    18601 ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt
    18661 tcaggagact gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg
    18721 ctgattaaca gtattttgtt aattgatgac aatctggcga tccattacgc caaccccgcc
    18781 gcgcaacaac tgctcgccca aagctcccgc aaattgtttg gtacaccgtt accggaactg
    18841 ttgagctact tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggt
    18901 tttaccgata acgaagtgac gctggtcatc gacgggcgct cgcatatcct ttctgtgacg
    18961 gcccagcgta tgccggacgg catgatcctg ctggagatgg ctccgatgga taaccagcgc
    19021 cgcttaagtc aggaacagct acagcacgcc cagcaggttg ctgcccgtga tttagtgcgc
    19081 ggcctggcac atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagctactc
    19141 agcaaagcgt tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg
    19201 gaccggctgc gaaatctggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt
    19261 accgaaagta ttcacaaagt agctgaacgc gtggtaacgc tggtgtcgat ggaactgccg
    19321 gacaacgtgc ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg
    19381 gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg
    19441 gagggtggtg aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag
    19501 cgctaccggc tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat
    19561 ttgcaggata cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc
    19621 ttatccatcg ctcgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg
    19681 ccagggcata ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc
    19741 aacgagggat agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg
    19801 cgctcgctgg ggcaggttta acctgtacga cgtttgagaa cggcgcagaa gtgctggagg
    19861 cgctggcgag caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg
    19921 ggctggcgct gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga
    19981 ccgcacattc cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc
    20041 tgcccaaacc gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt
    20101 accaggaaca gcagcagccg cgtaatattc agcttaacgg cccaacgacc gatatcatcg
    20161 gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta
    20221 ttagcgtgct gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc
    20281 atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct aaatatggca gctatcccaa
    20341 aagatttgat cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata
    20401 ccattcgtca ggggcgtttt gaacaggccg atggcggtac attattcctc gacgaaattg
    20461 gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt
    20521 accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc
    20581 agaatctcga acagcgagtg caggaaggta agttccgtga ggatctgttc caccgcctga
    20641 acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg
    20701 cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttactgcatc
    20761 cggaaaccga agctgctctg acgcgtctgg cgtggccagg caacgtgcgc cagctggaaa
    20821 acacctgccg ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc
    20881 ccggcgaact gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagct
    20941 gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc
    21001 tttccgaagc gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc
    21061 aggggcataa acaggaagcg gcgcggctac tcggctgggg ccgcaacacc ctgacgcgta
    21121 agttaaaaga gctggggatg gagtgattca cagcttgtgt gtaaagattg attattgagc
    21181 gcaaattgct ggtattttac gctttactgt tccgataagt tcagtatgat cttgcccgga
    21241 aaatggggag agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg
    21301 acagccacac accgcaaact gccgttgctg ccgtgctgtg tgccgcaatg attgggctgt
    21361 ttagttgagt ttcagattgc tgatgaagtg ctctttgttc atgccggatg cggcgtgaac
    21421 gccttatccg gcctacaaaa gcaatatttt caacaggttt tgtaggcctg ataagcgtag
    21481 cgcatcaggc aatcttgttc tcagcacatt gatggcgctt tcgttttcta ctttgtaaac
    21541 gaagcgccat tcactattta aatcacccgt gagaactgct gcatccgcgc tttctggcgc
    21601 agataggtat caaagcacat gcaaatgttg cggatcagca agcgaccttt cgccgtcacc
    21661 tgtattccct tctcatccac atccaccagc ccatcttttg ctaacggggc gagcagcttg
    21721 agatcttccg caaagtaatc agcgaagtgc aaatcccact gtttctcaat aggggcgtaa
    21781 tccagacgga agttgcagat gagcgactta atcacatcgc ggcgaataca gtcatcacgc
    21841 gttagcgcaa taccacgcca cagcgcattg ccttgttcat ccacttgctg atagtactgc
    21901 ttcaactctt tctggttctg cgcgtagcag tcgccaatca tgctgatggc ggaaacgccc
    21961 atccccagca gatcggtatc gccctgagtg gtgtagccct ggaagttacg atgcagcacg
    22021 ccttcacgct gggccaccgc cagctcgtca tccggacggg caaagtgatc cataccgata
    22081 aactgatagc ccgattgcgt caggaaggcg atggtttcct gcaggatatc gagtttttgc
    22141 tgcggactcg gcaggtcagc atctttgatt ttgcgctgag cagcaaaaat ggtcggcaga
    22201 tgcgcgtagt taaagacact cagacgatcg gggttcagct ccgccacacg tttcagggta
    22261 aaggcgaaac tctccggcgt ctgtttcggc aggccgtaaa tcaggtcgat gttggtggag
    22321 gtaaaaccaa tctcacgcgc atggttaagc agtgcaaaga tgaactcttc atcctgctcg
    22381 cggttaacca gacgttgcac ttctttgttg aagtcctgca cgcccatgct caggcgatta
    22441 aagccttcgg cgcgtaaatg atcgagtaca tccagttcga tttcccgcgg atcgacttcg
    22501 atcgaaatct ccgcatcggc attgaactgg aagttttcgc gcagcagctt catcaggcgg
    22561 ctgatttgcg ctttattcag atacgtcggc gttccgccgc cccagtgcaa ttggctgacg
    22621 tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt gctccagcgc gtccagatac
    22681 tgatcggcct tgtgctgctg gcgagtaaca atcttattgc aaccgcagaa gtaacaaagc
    22741 ttatggcaga acgggatatg tacgtagaga gataatggac gctcaggata gcgcgccacg
    22801 gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact ccagcgcggt cgggtacgag
    22861 gtgtatcgtg gcccggaata gttatatttc tggatcaggg ccagatccca gtcgatttgc
    22921 tgtacagaca tgctcactcc ttccgatggc gtctctggcg gctacggcgt accggcggat
    22981 gcccgtttcg ggtcatcagc cggttgcgca gccacttttg tcgccgcgac aaccgctgta
    23041 gtttaacgaa taaccacacc agataacata taaccggaag ggttatcagc agaacgggaa
    23101 ggcccacggc ggcaatccgt tagttgcccc gcagcagacg catcatgtct tcttgcttct
    23161 cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc gagtttctgc atcaactcat
    23221 caatacgatc cagtttagca tccacccaag attgctcttc ggcactcagg gtttcgcctg
    23281 cttccagacg ttccagcagc gcatccagac gctcatccgt ttccagtaat tccaactccg
    23341 cctgcggtga aagcataggt ttctcactct tcggtttgtg ctgtttggtg actttttcag
    23401 tcacgcccaa tggaataggg gttttactgc caatacgtgg atcttttggt gcgttctggc
    23461 ctttgctgcc tgacgtggtg tttccgcccg ctgcgcggct gcccggcgca tgaccacgac
    23521 gttttttctg acgcttgcga tcacgagctt cctgatccag ctcctcgcgt gtttttcgac
    23581 gcacttttgc atgacctttg ctgcgtgagt ttgaagatga tggtttcata atggttcgtc
    23641 ttttagccgt tttattcagt atagatttgc ggcggaatct aacagaaagc aagcaaagaa
    23701 aaaaggcgac agattactct gtcgcctttt ttcctgactc ataaccctta acgggtctta
    23761 caatccttgc aggccgccaa cacaccctgt ttttccttta gccagaacaa tccgtgttga
    23821 ttccatttcc gtttaattac gtctccggac gtttgtcttc ctgacaatcc tctgtcttcg
    23881 cctcctggcg ctcctgaccc tcatcctgag tcattgttcc tgttagcgtc ctcgctctat
    23941 gggtgctact ttacgccttt gctttaaaca aacaatcaac cgccatcagt atttgcacat
    24001 tttcagagtt ttatataaaa gcaattcact gatttatata gcttatttga catgcatcgc
    24061 agaattttct ctgctatttc gcataaagac tatcggcaat gtctcacaaa gcgtatggct
    24121 tttacttaca acgctcacgc cgaaaaatcc gccttttatt atggttcagg tataatcgcc
    24181 acaaagcttt gactgacgga gacgaccgct ttgactaatt tgaattatca acagacgcat
    24241 tttgtgatga gtgcgcctga tattcgccac ctaccttccg ataccggaat tgaagtggct
    24301 tttgcaggcc gttccaacgc aggtaaatcc agcgcgctga acacgctgac taaccagaaa
    24361 agcctggctc gtacctcaaa aaccccaggg cgcacccagc ttatcaacct gtttgaagtg
    24421 gctgacggca agcgtctggt tgacttgcct gggtacggtt atgcggaagt cccggaagag
    24481 atgaagcgca aatggcagcg tgcgctcggc gaatacctcg aaaaacgtca gagcctgcaa
    24541 ggtctggtgg tgctaatgga tattcgccat ccgctgaaag atttggatca gcagatgatt
    24601 gagtgggcgg tagacagcaa tatcgccgtt ctggtgctgc tgaccaaagc ggacaaactg
    24661 gcaagcggcg cacgtaaagc gcaattgaat atggtgcgtg aagctgtact ggcgtttaac
    24721 ggtgatgtgc aggttgaaac gttttcttcg ttgaagaaac aaggcgtgga caagctgcgg
    24781 cagaaactgg atacctggtt tagcgagatg cagcctgtag aagaaacgca ggacggcgaa
    24841 taattttctt gccttaatgc ttgtgccgga tgtggcgtat ccggcccgta aattcaatag
    24901 attacaattc taccgttggc ctgataaaca tagcgcatca ggcattacgg atcttttctt
    24961 tcgcccaata aaaaacgccc cagtcattac tgactggggc ggctaaaata ttcagccaaa
    25021 tccgattacg tgaagtaaaa ggtctgaaag atagaacatc ttacctctgt accctacgcg
    25081 actaacttta ctcttttttg ttcagttcag aaagcacttt ttgtaatttt ttttcagttg
    25141 ttgcatagga atttctaata gccatcacaa aacgtgacag cttatgttgc ttacttacga
    25201 aaaaagggca tgttcaggcg aatcttagtg cgcctgatcc cagttttcgc cactccccac
    25261 ttccaccagc aacggcacat ccagacgggt acagttttcc atcagttgat gaatctgctt
    25321 cgcgacggca tcaacatcat ctttatgaac ttcaaatacc agttcatcgt gtacctgcat
    25381 gatcatacgt acacgcggtt gctcagcctg taaccacgca tcaacggcaa tcatcgcccg
    25441 tttgataatg tcggcggcgg ttccctgcat tggcgcgtta atggctgcac gttcagccgc
    25501 tgcacgacga gcaccattgc tggatttgat atccggcaga tacagacggc gtccgtccag
    25561 cgtttcaacg tagccctgct ctttcgcctg agcacgggtg cgttccatat actccagcac
    25621 gccagggtag cgttcgaagt aaaggtccat gtacttctgc gcttctttac gtggaatgtt
    25681 caattgccgc gccagaccga aagcactcat gccataaatc agaccaaagt tgatcgcttt
    25741 cgcgctacgg cgttgctcgc tggtgacggt ttccagtggc aaaccaaaca cttctgccgc
    25801 cgttgcccgg tggatatctt ttccttccgc gaatgcggtc agcaagcctt tgtcacgcga
    25861 aagatgcgcc ataatgcgca gttcaatctg cgagtagtcc gctgagacaa tcacataatc
    25921 ctctggcgca ataaacgcct ggcggatacg acgaccttct tcgttacgca ccggaatgtt
    25981 ttgcaggtta ggatcggttg acgataaacg tcccgttgca gttactgcct ggtgataaga
    26041 ggtatgcaca cgcccggttt tcgggttgat catcagcggc agcttgtcgg tgtaggtcga
    26101 tttcagcttc gccagaccac gatactccag aatcactttt ggcaacggat agtccagcgc
    26161 cagttcttcc agtacctctt ccgacgttga cggcgcgcca cccggcgttt tcttcagcgg
    26221 tttaatgccc tgtttttcaa agagaatggt ttgtaactgc ttggtggaag aaaggttaaa
    26281 ttcctcacct gcaatttcat gcgctttctt ttccagctca gccagacgaa gggtgagctc
    26341 ttcagaatga ttgtgcagca ctttcggatc gatcttcaca ccgttacgtt caatgcgtga
    26401 aagcaccggc accagcggca tttcgatatt ctcgaagacg ttcaacggcc ctttgtgttt
    26461 ttgcagatcc ggccacattt tcagatgcaa ctgcaaggtg acatctgcat cttcggcggc
    26521 gtaacgtccg gcttcttcga gggcaatctg gttaaaggtc agttgatttt tgcctttacc
    26581 agcaatctct tcaaaagtga tggttttgtg cttcaaccaa cgttccgcga ggctgtccat
    26641 atcgtgacgc ccggcaacgc tattgagaat gtaggactcc agcatggtat caaacgcaat
    26701 cccacgcagt tcaatgccgt agttcgccag aataccgcga tcgtatttca ggttttgccc
    26761 gaccttcagc gccttttcat cttccagcag cggttttagc aactcgagtg cacgctcgcg
    26821 agagatttga tcgggcgcat caagataatc atgagcaacc ggaatatatg ccgctacgcc
    26881 tggctcgata gcaaaagaaa gcccgaccag gttagcagag atgttatcaa ggctgtcggt
    26941 ttcggtatca aatgcaaata ccggcgcttt ttccagcttc gcaatccacg ctttcagtgt
    27001 ttcttcatca aggatggtga cgtagttgtc ataagaaatc accgttgccg tcacttctgg
    27061 tgcttcgtct gcaacactgg tttcctgtgg cttcgcggct ggttttgccc ctttggcctg
    27121 taaccatttg cccgcttcga catcagcagt ccagcgtttg aactcatact ttttgaacag
    27181 ccccaacaac tcttccgctg ccggttgctg cacttccagt tgttcacagg tcagctccag
    27241 ttcaacgtcg gttttaatcg tcgccagctg gtatgagaga taagcaactt ctttgttttg
    27301 ctcgagcttc gctgccattg ttttcgcgcc acggaagctc aacccagcaa ttttttctgg
    27361 ctcggcatac agcgtatcca gcccgcccaa accttgcagc aatgcctgcg cggttttttc
    27421 accgacgccc ggtacgccag ggatgttatc ggaggagtca cccatcagcg ccaggaaatc
    27481 gatgatcagt tctggcggca cgccgtactt attcaccacc tcttccggtc cgaggatggt
    27541 attcgtcatg gtattgataa gcgtaatatt tggcgtcacc agctgcgcca tatctttatc
    27601 gccagtgctg atcagcaccg gacgcccggc tttttcggct tcgcgcgcca gagtaccgat
    27661 aacgtcgtcc gcttctacgc cagaaaccgc cagcagcggc agtcccatcg ctttaaccat
    27721 cgcgtgcaga ggttcgattt gtgctcgcag atcgtccggc attggcgggc gatgtgattt
    27781 gtaatgttca aacagttcat cacgaaaggt ttttcccttg gcgtcaaaga ccaccgctgc
    27841 atgcgtcggt ttatattgca tgatcagact gcgcagcatg ttgaggacac catacatcgc
    27901 accggtcggc tcgcctgcgc tgttagtcag cgggggaaac gcgtgatatg cgcgataaag
    27961 ataagatgaa ccatctacaa ggataagtgg attttggggg atctgaacca taatgtccgt
    28021 gcctgtttat cagattatgg gtaaaggatg ccacagacag gatgaaaaca tgagtttttc
    28081 accgcttttc gtgaaaatct tttgtagatc ttctggatcg ctcgcaaatc gtcatgtgga
    28141 taactttgtg cataaattat ttacatcgct tttaatttat tatcatgctt tttacagcat
    28201 gatgtttgtc tttgtttttc atgcagttaa aaataaagcc atccctaata attgagataa
    28261 tgggattttt tctaatgtgg atagatatga attatttttc tccttaagga tcatccgtta
    28321 tttgggtcgt tttctgctaa aatctgcgcc ttgcgcttct ttagcgcact gtctatggct
    28381 aaccttttga ataaattcat tatgacgaga atactcgctg cgataaccct tttgctgagt
    28441 atcgtattga ccattttggt cactattttt tgttccgtcc cgatcatcat tgccgggatt
    28501 gtaaaactat tgctacctgt gccagtcatt tggcgaaagg tttcgcgttt ttgtgatttt
    28561 atgatgtatt gctggtgtga aggtctggcg gtattactgc atctgaaccc acacctgcaa
    28621 tgggaagttc acgggctgga agggttaagt aagaagaact ggtatctgct tatttgtaac
    28681 caccgtagtt gggcagatat tgtcgtactg tgcgtgctgt ttcgtaagca cattccgatg
    28741 aataaatatt tcctcaagca acagctggcc tgggtgccat ttcttggcct ggcgtgctgg
    28801 gcgctggata tgccgtttat gaagcgttat tcccgcgctt atttattacg tcatccggag
    28861 cgacgtggta aagatgttga aaccactcgg cgttcttgtg aaaagtttcg cctgcatccc
    28921 accaccattg tcaatttcgt tgaaggctcc cgtttcacgc aagaaaaaca tcagcaaacc
    28981 cactccactt ttcaaaactt gttgccacca aaggcggcag gcattgcgat ggcgctaaat
    29041 gtactgggta aacaattcga taaactgctt aatgttacgc tgtgttatcc ggacaataac
    29101 cgtcagccgt tcttcgatat gttaagcggt aaactgacgc gaattgtcgt tcatgtggat
    29161 ttacagccta ttgccgatga gttacatggc gactacatca atgacaaaag ctttaagcgc
    29221 cattttcagc aatggctgaa ctcactgtgg caggaaaaag accgacttct gacgtcgttg
    29281 atgtcatcac agcggcagga caagtaaatt ttgctcgcct ctgaatgagc agaggcgagt
    29341 gagtatttta atgaacaaaa cgtccggcac gagacataaa ttcttcttcg ctcatctcct
    29401 gtccgttaaa aacaacttta cccggtgtat aacgcagttg cagcgaggcg gtattgttgt
    29461 caatcgtgat taactgaaac atctgaccta aggtcatcat cccgctgatt tgtttatcag
    29521 cccgcttttg cgctttttcc gcatccattc cttcagataa attaagctgt tttgcggttt
    29581 ctgtgacgac attaagcggt aacttcacat caaaattgag cgatttaata tctttgtttg
    29641 tggatgatga agatttggct gggtcggcaa tactgatatc cagattggca tttagttcgc
    29701 cgagtgcgtt cttccatctt accggttgtt taatggtcgg ctcacttttt tgtaataacg
    29761 gtaaatactc tttgaacaat gcagcattca cttcttgcag agcaacttca tcgtttgcta
    29821 actcagggtg tacagcaagc tgcttctgca tagcaatgtt atactggatg ataaattggc
    29881 gcactgctga gggatcaata gattcagcaa tcaatgacca ctcaccacta ccgagttgct
    29941 gattcccttt tgttagctta tcaagttcgt aggtcagatt gacattgata taatctttgt
    30001 ctttaacgcg atccagcatg gtttttgcaa cgaaagcatc gatttgggca acgtcttccc
    30061 cgtgattgat aatgttaatc tgattaaatt tcgactcgct ttcaccaacc ggaaatttct
    30121 tttcttctaa tcgcgccaga gaatcaatgg taaaactttt tgccgttact ttgttcattg
    30181 gtgataattg cagaacaata tcttcgactt tgccttcaat atccaggtta gacaatgaac
    30241 taccatcacc attaaaagta aactggccct caccaaaaga aaactttcca tactctgcag
    30301 gggcaacatc taatgtcgta gagaatgagc caccaaacgc gaatcgcaat gtcgcttcca
    30361 cgggcgattt atttttcgcg gcgataaata gcggttgtgt cagttcatta ttcactaagt
    30421 tcacttttgc cgctgccagt gctgggatga cattaccatg cattaacatt gtgatgggca
    30481 acggaccgtg ctcaacatcc acatcaaata caactttctg gcctggcttg atattgagat
    30541 cgggtgcacc gttatcgaat gtcatttgca tttgaaaacg agaattaaaa aaacctttgt
    30601 caaaattttt ataacttaac gtcatatcgt aggcatgttc acctttcttt ttagcatcgt
    30661 taaaatcttt aataaacttt tcgacaccag gctgaatttg cgtacctgtg taccatgtgc
    30721 caccacccca gattacggct aacgcaacaa taacacctgt agctgactta cgtatcatat
    30781 aaatattccc tgataaaaca gcaaaacttt aaa
//
LOCUS       DAZFAH010000035        30472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFAH010000035 DAZFAH010000000
VERSION     DAZFAH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.035"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..911
                     /locus_tag="ACHJWB_001496"
     CDS             <1..911
                     /locus_tag="ACHJWB_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFO4313149.1"
                     /translation="NKVTGADRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLF
                     LPTLQAATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKR
                     TVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFA
                     GKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQ
                     AEYQNRGTENDMIVFSPTTSDHPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENF
                     GRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(989..1945)
                     /gene="yjiA"
                     /locus_tag="ACHJWB_001497"
     CDS             complement(989..1945)
                     /gene="yjiA"
                     /locus_tag="ACHJWB_001497"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4313150.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(1956..2159)
                     /gene="yjiX"
                     /locus_tag="ACHJWB_001498"
     CDS             complement(1956..2159)
                     /gene="yjiX"
                     /locus_tag="ACHJWB_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4313151.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2209..4359)
                     /gene="btsT"
                     /locus_tag="ACHJWB_001499"
     CDS             complement(2209..4359)
                     /gene="btsT"
                     /locus_tag="ACHJWB_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4313152.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4752..5264)
                     /locus_tag="ACHJWB_001500"
     CDS             complement(4752..5264)
                     /locus_tag="ACHJWB_001500"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO4313153.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5282..6844)
                     /gene="hpaB"
                     /locus_tag="ACHJWB_001501"
     CDS             complement(5282..6844)
                     /gene="hpaB"
                     /locus_tag="ACHJWB_001501"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO4313154.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            complement(7095..7985)
                     /gene="hpaA"
                     /locus_tag="ACHJWB_001502"
     CDS             complement(7095..7985)
                     /gene="hpaA"
                     /locus_tag="ACHJWB_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO4313155.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHPL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            complement(7995..9371)
                     /gene="hpaX"
                     /locus_tag="ACHJWB_001503"
     CDS             complement(7995..9371)
                     /gene="hpaX"
                     /locus_tag="ACHJWB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO4313156.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            complement(9546..10334)
                     /gene="hpaI"
                     /locus_tag="ACHJWB_001504"
     CDS             complement(9546..10334)
                     /gene="hpaI"
                     /locus_tag="ACHJWB_001504"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO4313157.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            complement(10345..11148)
                     /gene="hpaH"
                     /locus_tag="ACHJWB_001505"
     CDS             complement(10345..11148)
                     /gene="hpaH"
                     /locus_tag="ACHJWB_001505"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO4313158.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11216..11596)
                     /locus_tag="ACHJWB_001506"
     CDS             complement(11216..11596)
                     /locus_tag="ACHJWB_001506"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO4313159.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(11606..12313)
                     /gene="hpaD"
                     /locus_tag="ACHJWB_001507"
                     /pseudo
     CDS             complement(11606..12313)
                     /gene="hpaD"
                     /locus_tag="ACHJWB_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
     gene            complement(12564..13094)
                     /locus_tag="ACHJWB_001508"
     CDS             complement(12564..13094)
                     /locus_tag="ACHJWB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313160.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            13285..13533
                     /locus_tag="ACHJWB_001509"
     CDS             13285..13533
                     /locus_tag="ACHJWB_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4313161.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            13535..15625
                     /locus_tag="ACHJWB_001510"
     CDS             13535..15625
                     /locus_tag="ACHJWB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4313162.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            15696..16628
                     /locus_tag="ACHJWB_001511"
     CDS             15696..16628
                     /locus_tag="ACHJWB_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4313163.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            16631..16852
                     /locus_tag="ACHJWB_001512"
     CDS             16631..16852
                     /locus_tag="ACHJWB_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313164.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            16865..17119
                     /locus_tag="ACHJWB_001513"
     CDS             16865..17119
                     /locus_tag="ACHJWB_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313165.1"
                     /translation="MNIQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            17121..17402
                     /locus_tag="ACHJWB_001514"
     CDS             17121..17402
                     /locus_tag="ACHJWB_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO4313166.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            17399..17671
                     /locus_tag="ACHJWB_001515"
     CDS             17399..17671
                     /locus_tag="ACHJWB_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313167.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFTELVAMKASALMKDMDMSSGVIKGNEFNQQG"
     gene            17676..17969
                     /locus_tag="ACHJWB_001516"
     CDS             17676..17969
                     /locus_tag="ACHJWB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313168.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            17981..18511
                     /locus_tag="ACHJWB_001517"
     CDS             17981..18511
                     /locus_tag="ACHJWB_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4313169.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            18609..19151
                     /locus_tag="ACHJWB_001518"
     CDS             18609..19151
                     /locus_tag="ACHJWB_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313170.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            19155..19688
                     /locus_tag="ACHJWB_001519"
     CDS             19155..19688
                     /locus_tag="ACHJWB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313171.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            19688..20203
                     /locus_tag="ACHJWB_001520"
     CDS             19688..20203
                     /locus_tag="ACHJWB_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313172.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            20207..20758
                     /locus_tag="ACHJWB_001521"
     CDS             20207..20758
                     /locus_tag="ACHJWB_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO4313173.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            20755..20940
                     /locus_tag="ACHJWB_001522"
     CDS             20755..20940
                     /locus_tag="ACHJWB_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313174.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            20979..21311
                     /locus_tag="ACHJWB_001523"
     CDS             20979..21311
                     /locus_tag="ACHJWB_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313175.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            21304..21501
                     /locus_tag="ACHJWB_001524"
     CDS             21304..21501
                     /locus_tag="ACHJWB_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313176.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            21491..21787
                     /locus_tag="ACHJWB_001525"
     CDS             21491..21787
                     /locus_tag="ACHJWB_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313177.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHVRWDEAEKAFHVRKKVNTV"
     gene            21784..22293
                     /locus_tag="ACHJWB_001526"
     CDS             21784..22293
                     /locus_tag="ACHJWB_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4313178.1"
                     /translation="MRGKLISAIHVAKRELALDDEAYTFALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            22363..22788
                     /locus_tag="ACHJWB_001527"
     CDS             22363..22788
                     /locus_tag="ACHJWB_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4313179.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            22860..23360
                     /locus_tag="ACHJWB_001528"
     CDS             22860..23360
                     /locus_tag="ACHJWB_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4313180.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            23395..23823
                     /locus_tag="ACHJWB_001529"
     CDS             23395..23823
                     /locus_tag="ACHJWB_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313181.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASVLVPDDVADRVRRAVCEIRACEAGADPR"
     gene            23807..24025
                     /locus_tag="ACHJWB_001530"
     CDS             23807..24025
                     /locus_tag="ACHJWB_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313182.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            24036..24263
                     /locus_tag="ACHJWB_001531"
     CDS             24036..24263
                     /locus_tag="ACHJWB_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4313183.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            24244..24552
                     /locus_tag="ACHJWB_001532"
     CDS             24244..24552
                     /locus_tag="ACHJWB_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4313184.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            24549..24839
                     /locus_tag="ACHJWB_001533"
     CDS             24549..24839
                     /locus_tag="ACHJWB_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4313185.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            24842..25423
                     /locus_tag="ACHJWB_001534"
     CDS             24842..25423
                     /locus_tag="ACHJWB_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO4313186.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            25423..27087
                     /locus_tag="ACHJWB_001535"
     CDS             25423..27087
                     /locus_tag="ACHJWB_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313187.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            27087..28676
                     /locus_tag="ACHJWB_001536"
     CDS             27087..28676
                     /locus_tag="ACHJWB_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO4313188.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLSIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVRDQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPGKQEKTEQTALAARLPEAKS
                     TPRDELDDMGDAVPARLLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            28660..29985
                     /locus_tag="ACHJWB_001537"
     CDS             28660..29985
                     /locus_tag="ACHJWB_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO4313189.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKAARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGNPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
     gene            complement(29985..30072)
                     /locus_tag="ACHJWB_001538"
     tRNA            complement(29985..30072)
                     /locus_tag="ACHJWB_001538"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            30111..>30472
                     /locus_tag="ACHJWB_001539"
     CDS             30111..>30472
                     /locus_tag="ACHJWB_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4313190.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPP"
BASE COUNT         7266 a         7725 c         8550 g         6931 t
ORIGIN      
        1 cgaataaagt aacgggggca gatcgcgtta cggagatcga ccgactgctt gagcaaaagc
       61 cacgcttaac tgctgatcag gcatgggatg ttattcgcca aaccagtcgt caggatctta
      121 acctgaggct ttttttacct actctgcaag cagcgacatc tggtttgaca cagagcgatc
      181 cgcgccgtca gttggtggat acattaacac gttgggatgg catcaatttg cttaatgatg
      241 atggtaaaac ctggcagcag ccaggctctg ccatcctgaa cgtttggctg accagtatgt
      301 tgaagcgtac cgtagtggct gccgtaccta tgccatttga taagtggtac agcgccagtg
      361 gctacgaaac aacccaggac ggcccaactg gttcgctgaa tataagtgtt ggagcaaaaa
      421 ttttgtatga ggcggtgcag ggagacaaat caccaatccc acaggcggtt gatctgtttg
      481 ctgggaaacc acagcaggag gttgtgttgg ctgcgctgga agatacctgg gagactcttt
      541 ccaaacgcta tggcaataat gtgagtaact ggaaaacacc tgcaatggcc ttaacgttcc
      601 gggcaaataa tttctttggt gtaccgcagg ccgcagcgga agaaacgcgt catcaggcgg
      661 agtatcaaaa ccgtggaaca gaaaacgata tgattgtttt ctcaccaacg acaagcgatc
      721 atccggtgct tgcctgggat gtggtcgcac ccggtcagag tgggtttatt gctcccgatg
      781 gaacagttga tcagcactat gaagatcagc tgaaaatgta cgaaaatttt ggtcgtaagt
      841 cgctctggtt aacgaagcag gatgtggagg cgcataagga gtcgcaggaa gtgttgcacg
      901 ttcagagata attaagcccg aatgccctca tattctggag gaatatgagg gtgataaagt
      961 ctcaggtatt gtgatgttgg agggctggtt actttttgag tcccgcaaac gccgcccgaa
     1021 tctcatcctc cggcaactga atcccgataa acaccatcgt gctatgcggt ttttcatcgc
     1081 cccacgggcg gtcccagtcg gcgctgtaga ggcgctggac gccctggaac agcaggcggt
     1141 taggttcgcc gtcaatccac agcatccctt tgtaacgcag cagtttatcc gccgattcca
     1201 gcagcaggtt ttccatcacc cgggaaactt cgctgatatc taccgggtaa tccagttcca
     1261 ccacaatcga cgaaatatcg ttttgtttgt ccgcgataaa gtggaaacgc ggtttggtgc
     1321 tgacgacgtt ttcttccagc ataaaaccgt tggtgttgaa cagcaggccc aggtcgatgt
     1381 cgccgtgggt aacggtgtag accggtgcac gggcgttgat gcgcgccagg cgttcacgca
     1441 gtttttctgc ttcgcctgcg acgtcggttt tagtcagcag aatacggtcg gcgtagccca
     1501 cctgggactg ggcgatggtg aactggttca tctgctcatc ggcatgtacc gcatccacca
     1561 gcgcaatcac gccgtccagc aggtagcgct ggcataaaat ctcatgggag aaaaaggttt
     1621 gaataatcgg gccaggatcg gccatgccgg tgcattcaat gaccagacga tcgaactgaa
     1681 tattgccctt gtcgagatta tccagcaggt cgagtagcgc gtcctccagc tcgttggagc
     1741 gcgaacagca gatgcagccg ttggtcagcg ttttgatctg cgttgcgcga tcaccaatca
     1801 attgatcatc aacggagact tcgccgaatt cgttttcaat cacggcaatc ttgtagccgt
     1861 gttgttcatt aagaatatgg cgcagcaggg tggttttgcc cgcgcctaaa aaaccggtga
     1921 gtagggtaac tgcaatcggg ttcatgctct ctccattaac aacagcgcat accgccttta
     1981 ccatcgccgc cgtagcgcgc gttctggcgt tcgcggaaga attcttcata gctcatgtac
     2041 ggcttgtcgg gatggttggt cttcatatgc tcgacgtagt tgtcgtagtc tggaatgcca
     2101 atcaacatct tcgccgcctg gccgagatat ttttttgcct gtcctaagtt accaaacata
     2161 gttcactctg ataagaacaa agccccgccg aagcggggct aaatacggtt agtggtgcga
     2221 agagatcttc acgccgcctt ccgggattgg aacgtacggt gtttctttgt cagtacgttt
     2281 gtcgctgtta cgcaccgcaa gccaggtttt gaaaccgtag aagatgatgc tgtacaccac
     2341 aatcaggaac agaatactca ggcccgcgtt ggtgtagttg ttcacaacga tgtggttcat
     2401 gttggcaatc tgctgcgccg tcaggtcagt accgttagca atcttctctt tgtactggct
     2461 tgccatgtag aagaagcctt ccatctgcgg gttggtgctg aacagtttca gacccagcgc
     2521 ccaggtggtg cagataagca gccatacagc cggaacaaca gttacccaga tgtattgggt
     2581 gcgcttcatc ttaatcagca caacggtgcc cagtaccagc gctacggctg ccagcatctg
     2641 gttggaaata ccgaacagcg gccacaggct cttaacgccg cccagcggat cgaccacgcc
     2701 ctgatacagc aggtagcccc acagacccac acagcccgca gtaccgatga taccggcaac
     2761 cagagagtcg gtttttttca ggaacgggat gaagttaccc agcaggtctt gcagcataaa
     2821 gcggccagaa cgagtacccg catccagcgc ggtcaggatg aacagggctt cgaacagaat
     2881 accgaagtga taccagaagc ccatgtcagc catcggcagc actttgtgga acacgtgagc
     2941 gatacctacc gccagcgtcg gcgcaccacc tgcacggttc aggacagaag gctcaccaat
     3001 gtctttcgcg gtttgcagga tctgctctgg cgaaatcacg aagccccagg agctgactgt
     3061 cgctgccgcg tgtgcggtaa cgtctttcag ctgcgccatg atgatcggcg cgttctcgcc
     3121 acccatttca tgcaggttag gcatggtgat gccaaggcca gcaggcgggg tattcatcgc
     3181 gaagtaaaga cccggttcga tgatggatgc agcaaccagc gccataatcg ccacgaagga
     3241 ctccatcagc attgcgccgt agccaatgaa acgcgcgtcg gtttcattag ccagcagttt
     3301 cggcgtcgta ccggaagaga tcagcgcgtg gaagccagat accgcaccac aggcgatggt
     3361 gatgaacagg aacgggaaca gagcgccttt ccatagcggg ccagtaccgt caatgtactg
     3421 ggtcatcgca ggcattttca gttccgggtt cagcaccacg atacccagcg ccaggccgac
     3481 gataacgccg attttcagga aggttgccag ataatcgcgc ggtgcgagga tcagccacac
     3541 cggcagcagt gcggaaacaa acgcatagcc aatcagcgcg aaggtaatgg tggtgtcttt
     3601 aaaggtcagt gccggacccc agtacggatc gtgagcaatc acgccaccga agtagataga
     3661 ggcaaccagc agcacgatac caatgacaga gacttcaccc acacgccccg gacggataaa
     3721 gcgcatgtag atacccataa acagcgcaat cggtacggtt gagcaaacgg tgaagacacc
     3781 ccacggactt tcggccaggg ctttaaccac aatcagcgcc aggacggcga ggatgatgat
     3841 catgattaag aaacagccaa acagcgcgat agtccccggt actggtccca tctcttcttt
     3901 gatcatctca ccgagagatg cgccgttacg gcgagaggag ataaacagca ccataaagtc
     3961 ctgaaccgca ccggccagta ctacccccgc cagcagccac agcgtgccag gcaggtagcc
     4021 catctgtgcg gcgagaaccg gaccgaccag cggaccagca ccggcgatag cggcgaagtg
     4081 gtgaccaaac aacacgtaac ggttggtcgg aacgtagttc agaccgtcgt tgttaataac
     4141 cgcaggcgtc gcgcgcgtgg ggtcgagttt catcaccttc tgggcgatgt acagactgta
     4201 gtagcgatac gccaccagat acaccgacac agaggcgacc acgatccaca gggcgctgac
     4261 gtgctccccc cgacgtaatg caactaccgc gaggcagaat gcaccgatga ttccgagaat
     4321 cacccagggt atgtgcttga atagtttttt cgtatccata gtaaaacctg gcattttact
     4381 cgtcgtcttt tggcttacag gtgcggcagt tgcgttcgtt cacccctgtc acttactgtt
     4441 gtaagctccc ggggattcac tcacttgctg ccttcctgca aaccaaaatt catagagtaa
     4501 tttgttaatc ataggccgaa gccgttctag gtttgcgttg cgtttgagga ggtaaattga
     4561 ccgctatcct gcagtgatgt tgccagagtt acgcgcgcgt aaagtaaggt aaataactga
     4621 gtggttattt tagggatgta agcggtcagg aatggcggtg agcggttggg caagaattat
     4681 tcatcgggtg gatatgtgat tgagatcaca ataaaaatgc cccttcgacg aatgcgtgaa
     4741 ggggctggag cttaaatcgc agcttccatt tccagcatca ccggatggaa acggcgttta
     4801 aagtagataa gtccgtgacc ttctgcactg aggatgatgt ttttaatctc caccagatac
     4861 accagatgtg tgccaattgc ctgcacatcg cggatctcac cttcaagact ggccagcgaa
     4921 ccttttagca ccggctgcgc cagcggacct ttttgccagc atgagaggct aaaacgctct
     4981 tccatcgcca tgcccgtcat gcccgcgaag tggcgtgcca tcagttcctg ctcatggttg
     5041 aggacgttga cgcacaactt gccgttgccc tgaaaaaccg ggttcatcgc actgttggcg
     5101 ttaatgcaca ccatcagcga tggtggtgta tccgtgaccg agcagacggc cgttgccgta
     5161 atcccgcatt gtccggcgtc gccctcggtg gtgataatat ttaccgctgc cgacaggctg
     5221 gccatcgcgt cacgaaagcg caggcgttgt tcatctaatt gcatcttaac ctcctgctgc
     5281 gttatttcag cagcttatcc agcatgttga tatcgtcgtt gttgtgcagg tgaggcacag
     5341 tccagccgtt ctggtcgtat tccgacaggc agcgatcaac catcgccatc atcttgtcca
     5401 tattgccgga gctttgtgcc tggcgcagac actgcaggcg aatctcatcc tggctaccgg
     5461 agtagttgat ttcatacagt tcgtgacgac caccaaactc gctgccaatg gcatcccaca
     5521 tcagtttgag gatcttgatg cgctggacgt gatccatacc gttcgaaccg cgcacatact
     5581 tcgccagata ctggtcgatc tgcggattgt tcaggtcacg ggcactggaa gggagataga
     5641 tcaggccact ggtaacgttg cgttcgataa tgtttttgat cttcgcgtag gccattggtg
     5701 ccagtacgcg ataggtttgc agtgcggcat gatccggtaa ataagccccg ttgacccacg
     5761 gcgtcgcttc agaacacatc gagtcactca atgcccagaa ggtgttgcgc cacgccacca
     5821 cttcaccgag atcggcctgc acaccacgga actccagggt gccggtacat tcgagtgatt
     5881 ttttcagcag tgccgtaatg aagtcgagtt tcactgccag gcgcacacag gcttgcagcg
     5941 gatacatacg ggcgaaaccg ccttccatcg tccagcgacg gcagcgatca aaatcgcggt
     6001 agatcagcac gttttcccat gggatcagca cgttatccat caccagaatc gcatcgttct
     6061 catcgaagcg gctggagagc gggtagtcat acggtgagcc ggtagcaccc gcgaccatct
     6121 cataagaggc gcgggagatt aatttgacgc catcggcatc cattggcgca acgaacatca
     6181 gtgcgaagtc cgggttttcg cccattactt gtgccgagcc gaagccaatc atgttgtagt
     6241 gagtcagcgc cgagttggtg gcaaccactt tcgcaccgct gacgataatc ccggcgtcag
     6301 tctctttttc cagcttgatg taaacgtctt ttactttatc ggtcggcaaa tgacgatcga
     6361 tcggtgggtt aacaatcgcg tggttaaagt agaggccagt ttcctgaata cgggtgtacc
     6421 agttacgggc gttctgctcg aactgaccgt aaaagcccgg attcgcgccc agtgcgcaac
     6481 cgaaagcagc tttgtagtct ggggtacggc ccatccagcc atagctcagg cgtgaccact
     6541 cagcgatggc gtcgcgttgc tggcgcaggt cgtcggcact tttcgccacg cggaagaatt
     6601 tatgggtata gccgccgctg ccggtgtcgg tgttccagca cagagagtcc tgcatctccg
     6661 gtttgtgcag tgcgtcgtac agctgggcaa cagacgctgc cgcattacga aatgccggat
     6721 gagtggtgac gtctttcact cgctcgccat agatatagat ctcgcgacca tcctgcaggc
     6781 ttttcagata ctcttccccg gtgaacggac gttgggtact ggcgcggaaa tcttctggtt
     6841 tcatgtcgac ctctacagta ttaatcggaa tacgattgtt aattttgtgt gttgtttttg
     6901 ttattcaatt gaagcttgca ggggcgatta cgggaagaca cttttgggac aatggctggt
     6961 acttttcagg tcgggtacgg ttttgtgatc gaggtcgctt ttagcgattt ttgacgttac
     7021 taaatcccct tgctccaggc ggagaaaggg gaatagcagg tggtgcctgg tttcccgtta
     7081 agactaaggg attttcacgt cacaggtact tttttggcac gataagcact gggcgagcaa
     7141 ccgactaagc gattaaaaaa gcgcgcaaaa taagctggat ccttaaaacc gagttgccag
     7201 gcaatattgt tcacggcgtt atcagaaaac agcagcagcc gcttggcttc tcgcagctgc
     7261 ctgtcgaaaa tcaaccgttt tggcggacgg ttggcaaagc ggcggcagat gtccgtgagg
     7321 cgtgattcgg tgatatgcag ttcgttagcg taatccggta ctgtccagtg ctgatgaaaa
     7381 tggctttcaa tcagcatatt aaaacgctgg aacagtttta attctccgcg cattccgctg
     7441 gcggcatggt cgtcgagttt tgcgttacgc agcagtaggg tgaacactgc ctgtgccagt
     7501 aacgtcaggg ggtgttcccg tccaggcagt tgttctaccg attcccgctc tatcagttgc
     7561 caatagtgtt ccagcgccgc cagttcgtcg ggtttatctg ccagtgacag gcaaatcccc
     7621 ggcaggccga aggtttcccg agtgcccgga taaagaactt ccagcagggg ccagatcaga
     7681 tcttcccgta ccgtcaacac atgaccgtcg gcgtcagact ccgtaataaa cgcatgaggt
     7741 actgacggcg gcgtcaggac aaacaggggc gcttccaccg agtagcgatg atcgtcaagc
     7801 tgtagctcaa tctgtccgct attgaggaaa tgcatctgaa agtactgttc gtggcgatgt
     7861 ggcagcatat ggcggccaaa aaatgccgcc atgcgggcga acgactgata atgcacatcg
     7921 tccgtgccca ggctttcatc gtactctttg ctgatatcaa tattggcaat ctgacggtca
     7981 cacatcgtcg ttccttacgg ggtcgctcgc ggacgggagg actgcattgg aatcgcccag
     8041 ataatccccg caccaatcac cagcagcgcg gcaacaaacc acaatccact gttaaagctg
     8101 ccggtcaaat ctttcaacca gccgatcata aacggactta acgctgaacc aatgttgcca
     8161 gtggcgttga tcaccgcaat accgatcgct cgtgcccgca ggctgatgga ctgatccggt
     8221 gttgtccaga aaatcgccat tgcgctgaat gatccggtcg aagccataat gatccccagc
     8281 atctggatca tgttgtgatc agttgccgaa gccagtaacc aaccagcggc agcgaacaaa
     8341 taaggaaggg cggtgtgatg ccttcgttcc tggcggcgat ctgagtgacg gctccagtag
     8401 accatcccga gaatggtaca aatctgcggt acggcggcca gcaggccgat ggtgatatta
     8461 ctgctgccct gattaaagct ttgtaggatc tgcggtgtcc agatgctgat cgcactaagt
     8521 gtgttggtca ggcagaaata cgccagggta tacatcatca ccaccggagt gaagatctcc
     8581 cgccacatgc tgcgttgttg catggcgtgg tggctgatgg ctccctctgg ctgaaccagc
     8641 gtcagacgat cgttatccat catctcttgc aggcattttt tgtcttcttt cgtcagccac
     8701 ttagctttgt ccggtgagtc atcaagccag aaccacacca tgacgccgag taataccgac
     8761 gggaagcctt ccagcaaaaa cagccactgc cagcctttta atgccattac gccatccagc
     8821 gacaaaatgt agccggaaac gatcgatccc aacgctgtcg ttaccggcat tgctaccata
     8881 aacaaggcgt tggcacgggc gcggaaatag gccggaaacc agaaggttaa atacagcaga
     8941 atgccgggca gaaagccggc ttcggtaatg ccaaccagta tacgcagtac gtacaagctg
     9001 gtggggccag tggcaaacat ggtggcagta gaggcgatgc cccaaagcac catgatggtg
     9061 gcgatccagc gccgcgcacc gacaatactc agcataatgt tgctgggaat gccgaagatg
     9121 acataagcgg catagaacag ggtggtagcg aggccaaaca tggtggcgct cagaccgagg
     9181 tcgcgtccca tcgtgagtcc ggcaaagccg atgttgatgc gatcaaggaa cgagaagata
     9241 aacagcacga acagaaagac gatcaggcgg cgaaacagct tcttaataac cgcctgttgt
     9301 ccggcagtca gcgctttatg ctgattcgcc ggatcgatac tctccggtat ggcaggtgag
     9361 gtgtcgctca tttttcttcc tctaatggga tttttgtagg gtacgacaag gtttatggcg
     9421 gatgcgcaca aacgccttat ccggtccgcg atttggtacg gctttttgcc ggatgcggcg
     9481 taaacgcctt atccggccta cggtttggtg cgagtgtagg ccggataagt gcagcgcatc
     9541 cggcattaat acacgccggg cttaacggtc gtggcctgtg cgccaaaccg tgctgccagc
     9601 gcttcagcgg cgcgggcgag cagggtggtg tcaactccga cggcgacaaa cagcgcgccc
     9661 agttccagat agcgttttgc cagttgctca ttggcgatca ggatccccgg cgctttgccc
     9721 gcttcgcgga tctgcacgat cgcctgctca atcgcggcct gtacttccgg gtgctgtgga
     9781 ttaccggcat aacccatatc ggcgctcaga tccgccgggc cgataaacac gccgtcgacc
     9841 ccttccacgt ccagaatctg cggtaagttc ttcattgcct cacgcgtttc gatctgcacc
     9901 agcacgcaca tttgatcgtt ggctttttgc aggtaatcag gaatacgatt ccagcgcgag
     9961 gctcgggcca gcgcactgcc cacaccgcga ataccggcgg ggggataacg ggtggcgcgt
    10021 accgcttcac gggcttcgtc agcgttttgt accatcggca ccagcaaggt ttgtgtgccg
    10081 acgtccagca gttgtttgat ttgcaccgga tcgttccacg acggacgtac caccggctgg
    10141 ctggggtagg gcgcaatcgc ctgtagctgg gtgagcacgg tttgcacgtt attcggcgcg
    10201 tgctcaccgt cgatcaataa ccagtcgaat cctgctccgg ccagtaactc tgcgctgtag
    10261 ctgctactca gccccagcca taatccgatc tgcgggcggc ctgctttcag cgccgcttta
    10321 aaactgtttt ccatcgttct ctccttaaac aaagcggcag ctaatggagc ccatgttgcc
    10381 gtaatcgacg tggaaggtgt cgcccttacg cgccggaacc gggcgggtga acgaaccgcc
    10441 gagaatgatt tgcccggctt ccagttgtac gtcatacggt gcgagtttgt tcgccagcca
    10501 ggccacgccg tttgccggat gattcagcac accagcggcg acgccggttt cttcaatcac
    10561 gccattgcga tacatcaggg cggagatcca acgtagatcc aactcatcgg gcttaatggg
    10621 acgaccaccg aggatcaccc cggcattggc ggcgttatca gaaatggtgt cgaacacttt
    10681 acgcgggcgc tgggtttccg gatcgatgtt gtggcagcga gcgtcgatca gctccagcgc
    10741 cgggatcaca tagtccgtgg cgttgtagac gtcgaacagc gtgcagtttg gcccacgcag
    10801 cggttttgcc agcacgaaag ccagctccac ttcaatgcgc ggcacgataa agcgatcggt
    10861 cgggatatcg ctgccatcgt ggaagaacat gtcatccagc agggcaccgt aatccggctc
    10921 gctgatttgt gagctggcct gcatcgcttt cgaggtcagg ccgattttgt ggcctttcag
    10981 cgtgcgacct tcggcgattt tcagtcgaac ccattcacgc tgcaccgcgt aagcgtcttc
    11041 gatggtgatc tccgggtaat ccagcgagat cgcgcggatc tgttcgcgct gtttttctgc
    11101 ctgatccaga cgctgggcga tcagggtgtg ggtgtgttta tcgaacatag cgatatcctg
    11161 tggcattgcc ggatgcggct gcgccttatc atccggcctg gaagctggtg caaatttatt
    11221 taaacaacgc gtgcacgttg ttttgtttaa aattcagcgt cgggtgcagc tctgcaatct
    11281 caaatgaaag cgccaggtaa cggctctcca tcagtgaggc gaagtgcgtt ttaatcagat
    11341 caaacagcat ttcgcccact tcctgacggc tttccaggct gcgcccgctg ccgattttta
    11401 gcgtcatatg cacaaaggcg taatcgtgct tgccatcagc catttgccag gtatcgatcc
    11461 agtgcgcccg gctacgtatt cccccaagcg gaaaaatccc cgttgccgcc agtgcagtgt
    11521 taacactggc aaacagctca ggtaagcggg cttcttcacg aatattttca gtacattcca
    11581 cgataaagtg cggcatggtg gttccttacg cgggaagcgg gaaaacagcg ttaacctgac
    11641 cggtgccaga gcttgggaat agctcggtga taaactccac cttgccgtcg tatttatccc
    11701 agccgagcat ccccagcagc atcaccgtgt cgtgcatatt gccttcgccg tagcagtagt
    11761 cggcgtactc cggcagcata ttgcagaact ctttgaactg gccttcgcgc cacaacttca
    11821 ccacgcgctc gtccatctgg cggtcgaact cgcgggtgta gctgttcatc ccttcttctg
    11881 cacgctgatc gtcaataaag cggtgcgata acgaaccgct ggcaaggacc gccacggtgc
    11941 cgtcgtactg ttcgatcgct ttcagaatcg cttcgcccag cttgcggctg tcagcaaaat
    12001 cgtgaaccgt gcagaaagcc gaaatggaga ccactttgaa gtgcttgtct tcattcatgt
    12061 agcgcatcgg taccagcgtg ccgtattcca gtttcaggct gggaatgttg tgcgcttttg
    12121 cccgcacgcc gagctttaag gcttcatcgg caataagctg ccccaactcc gggttgccct
    12181 cgtagttgta ggtcatatcg cggataaaat gcggcagctc gttgctggtg tagatgcctt
    12241 caaaatggtc tgcacagttg atgtgataag cactgttgac cagccagtgg gtatcgaaaa
    12301 cgataatggt atctgtaacc aggtgaatca tttgatacca acgagggttt tagtgaaagg
    12361 aggtgaaatc acctgtaaga tactgaaata aaacaggtga tgtctggatg agcgcttaaa
    12421 gtctatttga taccgattgt gcaataatta atcgtttatt ctaaaatcgg caaaactttt
    12481 gatactgcga tctcaacttt ttaaagcgtt taaaacttca ttaaatctat atggcaacta
    12541 acatacgggc cagcctcgcc aaactaaccc gctttagatt ctaccggatt tgattgccta
    12601 cttgtctcgg ttttttccaa aatctctctg acgcaatcgt ctggcatatc tacaatcatc
    12661 aacgcagcca ggcgcttcct gccttctagt tgtaataaat cctggttttc tttatccagc
    12721 agtgtcagaa ccgtctctac acctttgcga gcaaggagat tggttaactg ttctcgttcg
    12781 tctttcgtaa ggaatgaaag catctgtgta agttgatctc ccttatcacc agaacaacca
    12841 aaattaccac aaataccaac atcgtttggt gattcaccct tgccctcttt aagccactca
    12901 agactaactc cttcactttc agaaattcgt ataaggatac tttctctggg ggctggttgt
    12961 gtttgtcctc ccttgaaata gctgtttaat gtgttgatat tgattcccca cgctctggca
    13021 gctgcacttc ttgaagggta gcgctttgta agcattccaa tacgttcaat aacaggttct
    13081 tttccctgcg acataaagat acctcttggt gcttttctaa ggaaaggttt ctttctattt
    13141 atccaattga aaaataagga taaaagcaaa aattagccaa aataccaaaa ataccttgat
    13201 ttggtatttt tagtgatgca ttatttaccc gtgcagatat ccgcacgaat atccgcgcgg
    13261 gtaacttttt agaatagagg aagtatgagt agaagagaag tttcagatca cgattggccg
    13321 cctgaaatca tcaaggcgcg cttacacatg gctggcttat cactgcgctc tttatcttta
    13381 aaggctggtt acagcaggga ttcgctgaaa agcgtattac gtactccttg ccgaccgtat
    13441 cagcaaataa ttgctgatgc tcttggggta tcgcctgaag aaatctggcc cagtagatac
    13501 caggttaaga gctatatgag aaaggcgtca tgatatgtat gtcattgcaa aagaactgat
    13561 tggcgcgccc ggaatgcctg ctacaacaaa aggtattcgc caggcattac aacgttacgt
    13621 acaagggaaa agctgttgtt cccgtcgtcg ctcaggctct aaagcaactg aatacagcat
    13681 cgactgttta cctgaagtga cgcagcaggc attacgtgaa cgttatgccc tgcaactgat
    13741 gacgcaaaaa gccgatgaat caccggctcc ggtagtgaca aaggccaggc gctcacctgc
    13801 cgtggttgat gcggtggagg catatcgcgg atcaccacaa ctgatggtcg aacgccttaa
    13861 tgccctgact gaaaaccagc gccaggtggc tgatgcacga atcgcgatcg tcagcgaagt
    13921 tctgaaagtc gcgcaacaac ctggtttcag ctgtgcgaag gctatccggt ttatcgttga
    13981 caacctggca cgttcgcagc tggacgagcg cattgtggca atggttgaga cggcgaacgc
    14041 caaaaaggga aacagccgcg cgttaagtga aatcacgctg aaacgctgga ttgcggcctt
    14101 taacaaggcg cagaacgccg ctgaacgcct gcttttactg gcaccgggta agcgccagga
    14161 aataaaagcc gaagatatta actggctgcc cgaatttctg gcgcagtatc gccagtcaaa
    14221 cggccgacca atgaccgagg cttacgagga ttttgtcgct gaatggcagc accggcacgc
    14281 tgatgagcct tatatgctcg atatcatgcc gtcttatgac accattcgcc gcgcaatgaa
    14341 gaaactgccg gaagtggtga aacaaaaagg ccgggtgacc ggcagtgaat accgccagct
    14401 tgagggattc acgcgccgcg actggtcaaa aatgccggtg aattatgtct ggattggtga
    14461 cggtcacggc atgaagctga aatgcgcaca cccggttcac ggtcggccat ttgcaccgga
    14521 agtgaccttt gttatcgacg gtggcacgcg ctttgtggtg ggctggagcc ttgacctggc
    14581 tgaaaatgtt ttcgccgtag ccggtgccat acagcacggc attcgccatc acggcaaacc
    14641 gtttctgtat tactcggata acggctccgg ggaaaccgcc gacatcctgg ataaggaggt
    14701 tgtggggata ctgccgcgac tggggattaa tcacccgacc gggattgccg gtaatccgca
    14761 gggacggggc attatcgaac ggcttaaccg cacattaccg atgcgcatag cccgtaaata
    14821 ccgcacctat ttcgggaaag gtgcagatcg cgagacgtta cgcaaaacca accgcgattt
    14881 acgctcggca tttactgccc tgcaacaggg caaacggctg aacgcccggc agcagtcagc
    14941 gatgcgtgat ttaccgtcct ggtctgaact gattgatgcc attcgtgatg gtgttgagtg
    15001 gtacaacaac cggccgcacg atgaattacc ggtgaagccg aacggcaagc attacagccc
    15061 ggcggagttc agaaaaaaac gcctggcgga agaggacacg gaaattgaat ggctgtccga
    15121 tgtggaattg cgggacatgt tccggccgat ggtggaacgc cctgtaagac gctgtgaaat
    15181 acgctggctg aataatattt actacgcgcc cgagctgcgt gatgagcatg gccgcaaggt
    15241 gcttatcagc tatgacattc atgatgccga acgaattacc gtacgtcgcc tggatggcag
    15301 cgtgatttgc gaggcggtat gggacggtaa taaacgcgaa gccttcccgg ttagcgcgga
    15361 atactacaaa cagcagcaac gccttaaagg catgcgtaaa cgcgcagagg aaaaaatccg
    15421 tgatgccgag gatgaggttg tcaacgtgct ggagcacaag ccgcaggagc cctggctgga
    15481 aaacatctat cgcccggtag gtaatacggt ggccgttcag caacctgccg ttgatgatga
    15541 gcctgatgaa gaatacgagc gcaatttcca gcggggattg cagttgctcg aagcgaaatt
    15601 aaaagaaaat gacccgctgg cctgaaataa aaaataaccc gagcggcgac tcaggttatt
    15661 tgattaaaca aggtatcaaa tgagaggtta ataatatgac tgatattaac gatgtaatca
    15721 agaccattga tgaacttatt gatggcggcg tactgacgca gtatgccatc gccagagagg
    15781 cgggaatttc cgacggcaca ttatcggctt tccgcaaggg gaaatataaa ggcgataacg
    15841 ccgctgtggc tgcttccctg cgttcctggt atgagaactg gaataaacaa agcgcactgc
    15901 cggaaccgcc gcagtttgtg gaaacgcaga cagttcagga gctgcgcgca ctgtttcagg
    15961 cggttcgtct gatgggctgt attaacgtta ttgtgggcgt accgggtgtg ggtaaaacgg
    16021 ccactgcccg taattactgc caggagcaac caaacacctg gatgatcacc ctgtcacccg
    16081 cgcactccag cgtcacggag tgtctgctgg agctggccga tgcgctgggg attgattaca
    16141 cccgcgcgaa caaaggggca ttatcccgcg ccatccgccg tcgcctgatg ggaacgcgtg
    16201 gactggtgat tgtggatgag gcggatcatc tgggtattga cggtctggag caactccggg
    16261 caattcagga cgccacgggg atcgggatgg tgcttattgg taacccgcgc ggattgttta
    16321 aaggtggacg ccgcgccttt gatgatttat cgcgcctgtt cagccgtctt gcccgtacaa
    16381 aacaacttcg caaggccaaa aaggcggatg tgctggccat tgccagggcg tgggggatca
    16441 gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga gcgttacgcg
    16501 ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggc gcggcgctga
    16561 cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa ttactctcac
    16621 aggtgtgatt atggcggtat ttaatattcc tgatatttac ggacgctttt acctggttaa
    16681 tttcgataac gtgaaggtga tttcactggc cgaaaataaa gaatgtggcg atttactttt
    16741 tgaatttaat gaccgcacac gaatggtgat atctgccgga cttgatcgcg aaggtgcgac
    16801 agacgtttac agcggaatat gccgttctgt tggtgcgaaa caagtcagct aaatgaggtg
    16861 ttatatgaat attcaatcct gcggtaacaa aatgaattta ttcgactccc tgaacagcgc
    16921 gcgccgtctg accgaacttg ccggtgcggt actggaacgc agtaagcgct atccgcaacg
    16981 ttttgcactg aaaaccacgc cgccggtagg caacgtgcag ggaaccggtg aaattgaaat
    17041 caccatacag accaacggcc tgcgtcgccg tgtgaaggcc acccgcatca gcggctgcac
    17101 ggtttactgg gaggtatgag gtgaaaaaaa atctcattgc atgggcgtgg tcgagtggtc
    17161 ttattgagtt tggttacgtc ctgccggaag gtgcattacc gatagttgcc ggaaagcctg
    17221 ccacggtacg gcatgtgatt gaggttatgg cgcgtcatgg acgtgatgaa caggagcagt
    17281 tactggttcc ggggataccg gaagcggtga cggaggaaga agcctttaat gccatgattc
    17341 ggttctgccg tgaggtcaga cgccgggtca gttatccaaa cagaacgagg accagaggat
    17401 gagtaaagtt gtacgcatta ttttcgaata caaggagcac gttatccata aaaacgcgga
    17461 tggaacagtg cgcatggggg taagtctgga catacgttca accgggataa agcagaaagg
    17521 tgatggacct gccatgattt ttggggtggt tatgctcgcg gaaagcagag actttactga
    17581 acttgtggca atgaaagcca gtgcgctcat gaaagatatg gacatgagtt ccggggttat
    17641 taaaggtaat gaatttaatc agcaggggta attccatgag caaagtacgc gttatttttg
    17701 aatttgacca cgtaatgcat gaagtaaagc cagccggtaa tgacagtgag gaaatcacgg
    17761 agggggttac tgccacggta aaaatcgaac gtgatacaga gaacagaccg gcaggaccct
    17821 gtgatgttta tgcacaaatt cttaaatatc acagccccac aatcattcag tttctgacag
    17881 atgaactaca gggttccatg caggccatgg gggtaagcag ctccgttgaa cgccgttctg
    17941 ttcagaacgg accagataca ttgcaataag gaaaaacaaa atggcaaaac gcgttacaaa
    18001 attaaaggcc gcagcagagg cggcaccgca gacccgcgaa gaagtcagcc gcgatatccg
    18061 caccctgggc gatattcagc gagaggcgct gcgcctggaa acagcgatga atgacgaggt
    18121 ggcagaaatc accgcccgtt atacgccgca gattgaaaac cttaaaaaag aaatcaaagt
    18181 gctttttaag gggattcagg actggtgcaa aaccaaccgc gatgagctga cgaacggcgg
    18241 caaaacaaaa actgccaatc tgaccaccgg aacggtgtca tggcggctgg gaacgccatc
    18301 atgcagcgtc agccgtgatg tggaaggtgt gattgaaatg ctgcgccgta tgggtcttga
    18361 gcgattcatc cgcacgaaag aggaagtgaa caaggaagcc gtcctggcgg agccggatgc
    18421 ggtgaaaggg attgccggta ttaaggtaaa taaaggcgct gaaagttttt atgtcgagcc
    18481 ttttgaacag gacgccggac tgaacaaata acatcgcatt aaatctttaa atatcacatc
    18541 gttttaagta tggcgctcgc gtcaggggac tgcctgcgcc tgtaaactga aaataaggaa
    18601 caggatatat ggcatatttt tatttcaaat tagaccgtgt gcagacaaat aaatatttca
    18661 ccaaatatca acagactgtt ttaccgctac gcaacagtat tcttcgggca ttactgaaaa
    18721 atacaggcgc tgccggattg cgcttaaagc cgtttgccat ggacgtaatc agtgagtttt
    18781 atttttctgg tgctctgcct gcgggctggc gtaagcgcga tgatgtggct tttatcgggg
    18841 acggaccgtg ctttattgcc agacctgatg agtcatgccc tgaaggaccg gcgattgccg
    18901 caatgattga agccgctgaa cgcgagttaa gaaagcgtcc tgatttcctt gtctggctct
    18961 gtgaaaagct gggggtaatg agaatcccct ccatgtttaa catggactcc tggtggaccc
    19021 cgtcgctctc ccgtgatgcc ctgtgcgtgg tgtttaaagt aggcgcttat ggcagggaaa
    19081 taaaaggggg tattcctgaa gaatgccagg agattaaaca ttctgaatat gtggcgctga
    19141 cggaggaata attcatgatt gatgcaaaag tgcttgaagg ggttaaaaac tggctgagta
    19201 tttacgggcg tctgacctgc ggcattctgg ctgaaaaaat gaatatgccg ccatcctcga
    19261 tggtttattt tctgcgtgat gcggtcgatg ccggggtgct gacggaatgt aacggttttt
    19321 atgatattcc gcgtccccgc ccggtgcagc cggttcgtcg caaatgcagc caggaaggtg
    19381 cggctgatga tgttcagtgg tgcagtttca gaaaatccct gccgtggatt gaggggcatg
    19441 atattccgtc aatggcgtgg gaatttgctc agggcgttct gacctgtgaa accgtttatg
    19501 tggtggctga agttgatgag caggccatga aagaaggcgt gccccagttt gtgatggcgt
    19561 atatcgacat acgcctgggt gtcattatct gcggtttaag cggctggaat atcacctcgc
    19621 gcgttctgcg ttacctgatt gttgaccgga cggccgcgcc tgctgggata tctgcggagg
    19681 tggcgtaatg ttctttaaaa catcaaaccc ttccgcgctg gccgcgtggc aaaaatatca
    19741 gcaggactgc cagaaagtta aggatgaggc aaaacgcctt gaggccgtgc tgaatgttgc
    19801 gtgccggtcg gtatttgtat ccggtatcag tggcttttgt tttaaagggc tgcgctttat
    19861 ggatgacaaa tatccttttc atcgcgactt atggcgaaaa ccgactgcgt cgaatggctg
    19921 gagctgcaca ccgcgcacat cacgtattcc caaagccctg cgcgttgcct ctgacgaact
    19981 taacagtctg tggcgtgaat attcgcccgt cacgtatgcc agaaccgatg cactgttgtt
    20041 ctggctgggt attgatttct cggcaatcat gtttggccct gtgaagtggt tctgcgttga
    20101 cgatgtgatt taccttgagt gcggcgtaaa acccgcaaat cagaaaatga ccgaaattct
    20161 gtctgatgag ttttatgctg ctgaaaagcg agtcaggggg tgatgtatga tgattttaca
    20221 acccatgggg cgaaaaggtc aggcacctgc tcatgtccgt gcgtggacac ctgaagaaga
    20281 tgcgctgctg attgcgcttt atccatccac cccggttaag gatattgctg tcagggtaaa
    20341 aagaagtttc tggggtgtac ataaccggat tgttttatta cgcggtactt acccggagtt
    20401 gctcaaatgc aaacgtccaa gatttaaaca tgatgaagat aaatttatcc gaaaaaatgc
    20461 cagaacgatg accggtaagc aaatcggaga atatctgggg cgagaccggg attctgtcca
    20521 caatcgggcg cgatatattg gcgtaagcat gaaaaaatac ggagaattgc tgcccttcac
    20581 ccgtatacct gacgatgatg ttcatcttat tcgtgaatta cgggatgctg aatcaccacg
    20641 acgtcttacc ttccgggaaa taggcgagaa atttgaatta tccgagagta cggtgaattt
    20701 tatttatcac catcgccgga ctgccgagga cgctgtatta cgggagttaa cgccatgata
    20761 aagagacttg tatttttcgc aattttcgtt ccggtgttgg gggttggttt tatatttgcg
    20821 attacaggaa acctgtccat gatgccagat atttggttct ttatcaggat gtcgctcttc
    20881 ctgtttatta tgaatcttct tatcgacata tatatccgta ttactggaaa acataaatga
    20941 gaaaaatcac gtccttgtca gagttacagg agatgaatat gagtattgaa ttaagatcgt
    21001 cttatgagta tcgcaaattt cttatcgccg gaggcatgaa accggaagat gtggaaaaaa
    21061 tcgtttcttt tatggataag gaatgtgaca agcgggatgt gtcagaaatt attatggatg
    21121 acatgattct ggattcagtc gtggcgttaa gtccgttatg gattgtgcat tctcttgcgg
    21181 aaattgccaa aggcaccgat aaacaggcag ctgttgctgc ccttcagacg cttaatgaaa
    21241 tgcgtatatc tccacgcccg acattaatac atatgattct gtccagcatg gaggataaag
    21301 caaatgaata gtctacccgc tggatgggcg aggccactaa tggccaggaa gcatcatttt
    21361 tttaaaacag gcgaaaatat cagtatatgt ggacggtggt tatatctggc tcataatcgc
    21421 gagccggata catttgaaag ccctgatgac tgtgccgaat gccgcagaag agtgaataag
    21481 gagaaagata atggacaata gttctctgtt gttctggtgc ttatatatca catccttttt
    21541 cggtgcattt gttattaccc ggtggttatg ccggaaaatt atctgctttt ttgataaaag
    21601 acatccggtt gaacgggcgg ctgatgcgct cattcagcag gccattgtgt tatatagcgg
    21661 tgaatttttc tgccggataa caaccagaga tggctggcac ataatgatta ttccgccaac
    21721 acaccatgtc cggtgggatg aggcagaaaa ggctttccat gtccgtaaaa aggtaaatac
    21781 ggtatgagag gaaaactgat atccgccatt catgtggcaa agcgcgagct tgccctggat
    21841 gatgaggctt atacattcgc gctgctggcc gccaccggca aaaccagctg ccgggatatg
    21901 tcaccggatg agttatcccg cgtgctggat gttttcaaaa aacgcggttt taaagtgcgc
    21961 cagaagccgg ttaaccgggc cttaaaaccg ggtacggtga ccgccaaaat tcgtgccatc
    22021 tggaaggtga tgcaccggca gggctttatc tctgatggtg cggaaaccgc ccttaaccgc
    22081 tgggtgaaat cgcagacggc cgcgcagaac ggcggcgaag gtgtggcaaa ctggcagtgg
    22141 ctggagcaac accccgccct ggcctcagat gtgctggagc gtctcaagcg atggcaccgc
    22201 cgcaaaatgc tggccgcgat gggaatgccc gaacgcacgc tgatggggta tgacgccgtt
    22261 tgcaggcagt atgaaaaatc acttccccgt taaccccaaa tcccgccaca acgcgggatt
    22321 tttattttaa acttaccggg aacgcgagaa ccggaggctg atatggcaga aactcagatg
    22381 agcatgtttg gtggtgacag tgagcaactg cacgccctta tcgaccgcct tgatgacatc
    22441 cctgatgatg ttctcaaaaa gaactggccg cggaccctgt cagaactggt tgaagtcacc
    22501 ggcgcagaac ttcagcgtca ggggattgag ccggtactgg ccggtaaact ggcacgcaag
    22561 gtggctgcgg ctcaggcagc ctatatgggc ggacggggtt attacctgcc ggtcggggaa
    22621 tctctctttg ccgagctgcg aaacaatgag atattttcgc gctgggaccg gggcgagaaa
    22681 attgaatccc ttcgccgcca ttaccggatg tcagaaaccc agatttatac cgtcatacgc
    22741 gaacagcgcc gtctgcatct ggcaagaacg caaccgccac ttttctgata ttccgcaaag
    22801 cctgccgcct ctccttcacc gttacgctga ctcagagaac atcatgagac agcgtaacaa
    22861 tgccaaaact ccccgcacca ctgcgtaaaa agctgattgc ccttgttctg gccggtgccg
    22921 ggacgttcac gattgccacg cattacaccg gttactggga agggaaagaa aactccacgt
    22981 atatcgatcc caccggtaca cccaccatct gttacggcca taccggcccg gatgtgaaac
    23041 cgggtatgac cctgacagat gaagaatgcc tggaactact ggaaaaggac atgaaatggg
    23101 cctttgcggc cattgatcgg cgtgttcagg tgccgcttac ccgtggtcag acggtggcgc
    23161 tggcttcgtg gatcttctgg gccggtgaaa cgaactttcg caactccacg cttctgcgcc
    23221 tgatcaatgc cgggcagatg cccgcgtcct gtaagcagta tatccgctgg atttattcaa
    23281 agggggtgaa actccccggc cttgaggccc gccgttcggc ggatgaatgg ttatgtcgct
    23341 acgacttgcc gaaagtctga accgcttctg gcgaccgctc atgatagcgc tgctgtgtgc
    23401 ggtactgctg ttacggggtg tcctgtggct gcggtgaata atgccttacc ggccctgatt
    23461 gccggggcgt gcatggcggc gctggggatt atcgctgtac tggttgccgc tatatgggga
    23521 atgcaccaga agacgcagcg ccttgaagac aataatcagg tgcttgtgcg tgagcgggat
    23581 gaggcgcgtc aggtgctggc aaatcagcag cacaccctgc aactcatttc acaaatcagt
    23641 gaggcggcca cgaatgaaaa acaacagaac attcagcaca gcgaggggca gcagagcgtt
    23701 gtccgccggt cgctggcggc agtgcctgcg gcctctgtcc ttgttcctga tgatgtggct
    23761 gatcgggtgc gccgggccgt ctgtgaaata cgtgcctgtg aagccggtgc cgatccccgc
    23821 tgaatggctg gctgactgcc tggtccctcc tgcgccggag ccgttcacgt ttggggcatc
    23881 ggtcacttac aacctgcaac tgctggcggt gatcaagaac tgcaacgtgg acaaggccag
    23941 tattcgtcgt ctggaggcgc ggcgacaaca tgaatttact gatatggccg gaacgcctgc
    24001 tgttccggca ggaaagacga agtaaggaaa aaggtatgga tgattcagat cgtgctcagg
    24061 ctgtgatgga gcggggatca gaacgcgccc tttgtaaccg gctgacacgt aagcgccggg
    24121 caatggatgc tccgggcaga cgtgtctgcg cggactgtgg cggagagatc cccgccgcac
    24181 gtcttgttgc tgtgccggat gccatccgtt gtgtgaactg ccagaacatc atggaggccc
    24241 gtcatgtggg tcagcatcgt taaagattat gttgtgccga tcctttcggc gacggcaacc
    24301 gctggcggga tattcatggc gctgatgcgc aaaacgtttg tcccccgcga ggcctttgaa
    24361 aaactctccg accgcgttga gaaggtggaa acccgcctgt cgtcactgcc gacagaagcc
    24421 gaggtcaacc gtctgaatgt ggaaatcgtg accctgcggg gtgaactgaa aaccacgaac
    24481 gccacgctcc gctctgtctc ctatcagaac gaactgctgc tggagcaggc tgtaaggaaa
    24541 aaaacgcaat gagtgatttc attactgaag atcagcgtct tgtcattctg cggtcgctgg
    24601 cggattacaa cggcgaactg ggtgaatccg tgctccagga ctgtctggat gattacggtc
    24661 accgcgtgtc ccgcgacacc gttcacaccc acattgcctg gcttgccgag cagggactgg
    24721 tgcgtaagcg cacgctcgtt aacggttatt tcatcgccga actgaccggc cggggtcagg
    24781 atgtggcgga aggtcgcgcc agcgttccgg gtgtgaagaa accacgcgca agggggtaac
    24841 gatggacaag ccgacgcgtg gacgcgtgcg caaggtggat ttgctccccg acagcatccg
    24901 taagccactg ctggaaatgc tgcgtgaaaa acgtctgacg caggtccaga tacgcgagga
    24961 aatcaaccgt ctgatccgtg aggcgggcct gccggaagag cagcagcttt caccggcggc
    25021 catcagccgg gaagcctccc gtaatgagct gattgcccgt aacctgcgtg atttacgcga
    25081 gcagacaaaa gccatgatgg ccgaactggg agacaagccg accggtgaaa ccacagcgct
    25141 gattctggag atgtcccgcg ccctgatgta ccgccgtctg cgtgccgcga ccgaatccct
    25201 gaacagtgac agcgacgtgg atatgcgtct tattaaggac atcctcctgt cagcacagcg
    25261 tgcggaaagt gccgccgagc gcagcattaa gcgtgaaaaa gaaatccggg ccgcatttgc
    25321 tgaagagatg gcaaacgccg tcaccgacga gctgcgcggc gtggacggga tgagtgaaca
    25381 gcttgagtcc cgtattaagg ggattctgtt gggtaaagcc tgatgaataa cgccgaagaa
    25441 ctctccccct tactgacgaa taccgtctcc acccgcaaaa ttgatctggc cggtgaaaaa
    25501 gccctgctgg gcgtggatgt gccggacagc cttgatttgc ccggtgatat gccggtattt
    25561 ctggattatc aggcccgctg gtttgaggat gaaagcgagg tctgcatcgc ggaaaaatcc
    25621 cgccgcaccg ggcttacctg ggcggaagcc gggcgtaacg tcattaccgc cgcaaagcct
    25681 aagcggcgtg gtggccgcaa tgtgttttat gtggggtcaa agcaggagat ggcgctggaa
    25741 tatatttctg cctgcgcact gttctcccgt gcctttaacc agctggcaga tgctgatgtg
    25801 tatgagcaga ccttctggga tcgggataaa aaagaagaaa ttctgaccta catgatccgc
    25861 tttccgaaca gtggattcaa aattcaggca ctgtcttccc gtccgtcaaa cctgcgcggc
    25921 cttcaggggg atgtggtgat tgatgaagcc gcgttccacg agtcactgga tgagcttctc
    25981 aaggcggcaa tggcgctcac catgtggggc gcgcgcgtgc gtattatctc cacgcataac
    26041 ggcgtcgata atctgtttaa tcagtacatt caggaagccc gcgaggggcg caaggattac
    26101 agcgtccacc gcattaccct ggatgatgcc attgcggacg ggctgtaccg tcgtatctgt
    26161 tatgtcaccg gccgcgaatg gtcaccggaa agcgagcaga agtggcgtga tgatctctac
    26221 aaaaacgccc cgacccgtga ggatgccgac gaggaatacg gctgtatccc gaaaaaatcc
    26281 ggcggtgcct atattcctca cgcgcttatt gaaatggcga tgctccgcga catcccgatt
    26341 ctgacgtttg aagccccgga cgacttcatc agccgtgccg catggctgcg tgaatcggag
    26401 gttttaacct ggtgtgaaga acatttaaaa ccgcttttag aggcgttaaa tccccgttcc
    26461 cgcttcagct tcggggaaga ctttgcccgc accggtgacc tctcctgctt tgtgctgctg
    26521 gaaatcactg aatccctggc aaaacgcgag gtgtttcgcg tggagctgcg taacctgcca
    26581 tatgcccagc aggagcaggt gatgatgtac atcctgaccc gcgttccggc gctggtcggt
    26641 gcggcgttcg acgccaccgg taacggcggc tatctggccg aagccgcgct gctggccttt
    26701 ggcccggaca tcatcgactg cgtgatgtta tcgccgaagt ggtacggcga gtggatgcca
    26761 aaactgaagg ccgagtttga ggatcagaat atcctcgtcg cccgccatca gaccacgctt
    26821 gatgatttgc gccatgtgaa ggtggtgaac ggtatcccgc aaatcgacaa ggggcgtacg
    26881 aaggaccaga acgcaacggc tgtgaacgcc cgccgccatg gtgattttgc cgtggcgctc
    26941 tgtatggcaa accgggcgtc atacatggag ggcttcatcc tggatgaatc agcctgtcag
    27001 gcgctgccgg agcggtcgcg ggcaatggag ggcggttatc gtgatgacga tgaggcatat
    27061 catgaatttg atcgggggtg ctggtagtgg gacgcataat tgatcttgac ggaaaacctt
    27121 tctcctttga cccagagatg cagagtgccg tgctggatat tccgcagatt gccagccgtt
    27181 atattgaaca tccggcctcg ggtatcaccc cgaaccgggc ggcacagtgc cttcgcgggg
    27241 ctgaacgtgg cgatctgatt gcccagtccg atctggccgc tgacattgaa gaaaaggata
    27301 cccacctttt tgcagagctg ggcaagcgac gtctttccat tcagggcgtg ccctggagta
    27361 ttgagccgcc accgaacgcc agcgcgaatg agaaaaagga cgcggaaatg ctcgacgaat
    27421 atctgcattc cgccgactgg tttgatgcca tgctgtttga tgccaccgat gccatcctga
    27481 agggctattc ctgcatggag attgagcacg ggatgctcgg taaaatgcac atcatccgcg
    27541 ccatccgctg gcgtgacagc gggcatttct gcctaaaccc ggatgatttg agcgaactgc
    27601 ggctgcgtga cggcagccat gccggggtgg cgtttcagcc ctttggctgg atagtgcatc
    27661 agtcacgttc acgcaccggc tacggtggtg cgacggggct tgtcagaacg cttatctggc
    27721 cgttcatttt caaaaactat tccgtgcgcg atctggctga atttctggag gtgtacggcc
    27781 tgccgatgaa ggtcggtaaa tacccgtccg gggcaacgcc ggagcagaaa agcgccctga
    27841 tgcgggcggt gatggatatc gggcgacgta caggcgggat catcccggcc gggatgtctc
    27901 tggagtttca ggcagccgcg aacggtcagg ccgatccgtt tgaaaccatg atttcgtggg
    27961 gggagcgttc catctccaaa gctatcctcg gcggcacgct gaccacggaa gccggagaca
    28021 agggcgcgcg ctcgctgggt gaagtgcata acgaggtgcg ccgggaaatc agggattctg
    28081 atttacgtca gctggccgcc acgctgaacc gcgatctggt gtatccgctg tatgccctga
    28141 acaccgccca cgccatagat atccgtcgcc tgccgcgtat ctgtttccag acaaaagagc
    28201 cgggggatat caccaaaatc accagtgcgg tgatgcagct cagtacgggg atggatattc
    28261 ctgatccctg ggtacgggac cagaccggta ttccacagcc tgcccccggt gaagccatct
    28321 tccgtgtccg gcagagtggc aatgaacctg ctcagacaga caaagagatg ccaccgggaa
    28381 aacaggagaa gacagagcag acagcgctgg ctgccagact gccggaagca aaaagcacgc
    28441 cccgtgatga actggacgac atgggggatg cggtgcctgc ccgcctgtta caggaggcca
    28501 tagacccggt gcttgagccg gtcattgatg ccatcagaac gcgggggctg gcggaggcac
    28561 tggcagacct gcctgccctt taccgtgaaa tggatgattc ccgcctgatg acgctgctca
    28621 gtgatgccat gtttgctgcg gaaatgaagg ggatgctgga tggcacaggg gattgattta
    28681 ggttatgccg ccacacttgc ctcaaaagag gcggtggcat acttccgcgc caaaggggcg
    28741 catatcagct ggaactggtt cgaaacagac gcggatgttc atgcccgttc attcacggcg
    28801 gcaaaagcgg cacgcctgga cgtactgact acactacagg cggaagtgca acgggctatt
    28861 gatgagggga tttcacagaa agcatttatc cgcacactga ccccccgcct gcaaaagctg
    28921 ggatggtggg gaaagcagat tgtggtggac agcgctggta acgcggaaga agtgcagctg
    28981 ggtaatcccc gccgtctggc gctgatttac aacgtgaaca cccgcgtggc ttacaatgcc
    29041 gggcgttaca cgcagatgat gaacaacacg gacacgcatc cgttctggca gtatgtggcg
    29101 gtgatggaca gccgcacccg cccggcgcat tccgccctta acgggctggt attccgctat
    29161 gacgatccgt tctggaatac acactacccg cccaatggct ggaactgtcg ctgtcgtgtc
    29221 cggccattgt ctcaggcccg tctggatgcg atggggctgt ctgtttcatc cggtcaggat
    29281 catctctcca cccgcaatgt tgaggctggc gtggataagc agaccggaga agtcagagaa
    29341 atgccggtga ccacatattc agatggcacc agaaccatga caccggatgt gggctggtca
    29401 tataaccccg gttcggcggc gttcggcaca gaccaggcgc tgatccgtaa actgatcgag
    29461 gtgaaaagcc cggcgctgcg ggaaatggtg gttcaggaga tgaacaacag cccggagcgg
    29521 caactggcgt tccgcatctg ggcaaaaaac atcatgaaaa cccggcgagg cggtaacgat
    29581 atccgcacgc tgggctttat gaccgaaagt attgcgcagg cggtggaaag ccggacggga
    29641 acgccaccgg cccgcctgct ggcgatgagc ggtaaaaatg tgctccatgc ggacagcatg
    29701 aaacatcaga atgacggtat cgccctgacg ccggaggatt tcgcgcagct tcccgccatg
    29761 ctggctgccc ccgatgccgt gctgtgggat catgtccatc agaacttgct ttacatcact
    29821 gaaacccgtg acggaacggc aaagattgcc gtcaatgcgc cttatagtgt gaaacgtcag
    29881 ccggataagc tggatgtggt gattaatgcc tatagagtga gcaggaatac tctgattgat
    29941 gctgttcagg gaggtaagat ggagatcctc gaagggaaat tgtaagtcgg tggtgggggt
    30001 cgaacccaca taaacatcct tcgggtgaag atatccctca ggtgccgctt taccagttaa
    30061 gagtacaccg acctgctttt attttaacca ggtagtttca cggaggcaat atgtcatcca
    30121 ttgatgccgc tgtcgttgtt gacgttacgc gtctccagcg ggtctttgcc cggcttcagt
    30181 ttgtgggtgg tggaaaagac ctggcccgca gtgtggcggc gagtcttctg tcatcgtcag
    30241 aaatggcgtt tgaacaggaa aaagagccgg acggcgaacg ctggcatgac tggtcagatc
    30301 cttaccgcaa gtggcgtacc cgtaagggat acatgcccgg caaaatcctg acgctgaacg
    30361 gcgatctggc ccgacgcctg accacggatt atggcgacac ctgggcgctg attggatcaa
    30421 atgagcctta tgcggccatt catcagtggg gtggcctgcc cggtatgccg cc
//
LOCUS       DAZFAH010000036        30204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFAH010000036 DAZFAH010000000
VERSION     DAZFAH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.036"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHJWB_001540"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHJWB_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4313191.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYGITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHJWB_001541"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHJWB_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4313192.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHJWB_001542"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHJWB_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4313193.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPEQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHJWB_001543"
     CDS             complement(1087..1581)
                     /locus_tag="ACHJWB_001543"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4313194.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGELPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHJWB_001544"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHJWB_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4313195.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTCG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHJWB_001545"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHJWB_001545"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4313196.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHJWB_001546"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHJWB_001546"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4313197.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHJWB_001547"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHJWB_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4313198.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHJWB_001548"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHJWB_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4313199.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHJWB_001549"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHJWB_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4313200.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAVLKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHJWB_001550"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHJWB_001550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4313201.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHJWB_001551"
     CDS             complement(9275..9769)
                     /locus_tag="ACHJWB_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4313202.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHJWB_001552"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHJWB_001552"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4313203.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHJWB_001553"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHJWB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4313204.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHJWB_001554"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHJWB_001554"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4313205.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIDGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHJWB_001555"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHJWB_001555"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4313206.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHJWB_001556"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHJWB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4313207.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHJWB_001557"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHJWB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4313208.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHJWB_001558"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHJWB_001558"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4313209.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHJWB_001559"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHJWB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4313210.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKERLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHJWB_001560"
     CDS             17948..18874
                     /locus_tag="ACHJWB_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO4313211.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHJWB_001561"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHJWB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4313212.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHJWB_001562"
     CDS             complement(19225..21444)
                     /locus_tag="ACHJWB_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4313213.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHJWB_001563"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHJWB_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4313214.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHJWB_001564"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHJWB_001564"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4313215.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHJWB_001565"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHJWB_001565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4313216.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLMLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHJWB_001566"
     CDS             complement(26816..27298)
                     /locus_tag="ACHJWB_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4313217.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHJWB_001567"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHJWB_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4313218.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHJWB_001568"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHJWB_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4313219.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHJWB_001569"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHJWB_001569"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4313220.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..>30204)
                     /locus_tag="ACHJWB_001570"
     CDS             complement(29946..>30204)
                     /locus_tag="ACHJWB_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO4313221.1"
                     /translation="FFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTPLI
                     ILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7448 a         8077 c         7637 g         7042 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgccgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgc
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccgattt tttcactcac tttgcgcagc ggccagagtt gctccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aagtcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gtaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cacgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa caccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc accaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtaa cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctctcacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgtcaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcctt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccaaggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 attgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagagaga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaagta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg actaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaaaggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacatta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggc
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagccact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgggcgggt
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atatggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catgggtacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtgg tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct
    29761 tctatcgccc acccggacaa accgctaccg gcagcgggct tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg aatttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaa
//
LOCUS       DAZFAH010000037        29650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFAH010000037 DAZFAH010000000
VERSION     DAZFAH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.037"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..1880)
                     /gene="glmS"
                     /locus_tag="ACHJWB_001571"
     CDS             complement(51..1880)
                     /gene="glmS"
                     /locus_tag="ACHJWB_001571"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4313222.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2042..3412)
                     /gene="glmU"
                     /locus_tag="ACHJWB_001572"
     CDS             complement(2042..3412)
                     /gene="glmU"
                     /locus_tag="ACHJWB_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4313223.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3763..4182)
                     /gene="atpC"
                     /locus_tag="ACHJWB_001573"
     CDS             complement(3763..4182)
                     /gene="atpC"
                     /locus_tag="ACHJWB_001573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4313224.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4203..5585)
                     /gene="atpD"
                     /locus_tag="ACHJWB_001574"
     CDS             complement(4203..5585)
                     /gene="atpD"
                     /locus_tag="ACHJWB_001574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4313225.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5612..6475)
                     /gene="atpG"
                     /locus_tag="ACHJWB_001575"
     CDS             complement(5612..6475)
                     /gene="atpG"
                     /locus_tag="ACHJWB_001575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4313226.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6526..8067)
                     /gene="atpA"
                     /locus_tag="ACHJWB_001576"
     CDS             complement(6526..8067)
                     /gene="atpA"
                     /locus_tag="ACHJWB_001576"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4313227.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8080..8613)
                     /gene="atpH"
                     /locus_tag="ACHJWB_001577"
     CDS             complement(8080..8613)
                     /gene="atpH"
                     /locus_tag="ACHJWB_001577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4313228.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8628..9098)
                     /gene="atpF"
                     /locus_tag="ACHJWB_001578"
     CDS             complement(8628..9098)
                     /gene="atpF"
                     /locus_tag="ACHJWB_001578"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4313229.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9160..9399)
                     /gene="atpE"
                     /locus_tag="ACHJWB_001579"
     CDS             complement(9160..9399)
                     /gene="atpE"
                     /locus_tag="ACHJWB_001579"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4313230.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9446..10261)
                     /gene="atpB"
                     /locus_tag="ACHJWB_001580"
     CDS             complement(9446..10261)
                     /gene="atpB"
                     /locus_tag="ACHJWB_001580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4313231.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10270..10650)
                     /gene="atpI"
                     /locus_tag="ACHJWB_001581"
     CDS             complement(10270..10650)
                     /gene="atpI"
                     /locus_tag="ACHJWB_001581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4313232.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11267..11890)
                     /gene="rsmG"
                     /locus_tag="ACHJWB_001582"
     CDS             complement(11267..11890)
                     /gene="rsmG"
                     /locus_tag="ACHJWB_001582"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4313233.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11954..13843)
                     /gene="mnmG"
                     /locus_tag="ACHJWB_001583"
     CDS             complement(11954..13843)
                     /gene="mnmG"
                     /locus_tag="ACHJWB_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4313234.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14225..14665)
                     /gene="mioC"
                     /locus_tag="ACHJWB_001584"
     CDS             complement(14225..14665)
                     /gene="mioC"
                     /locus_tag="ACHJWB_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4313235.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLL"
     gene            complement(14755..15213)
                     /gene="asnC"
                     /locus_tag="ACHJWB_001585"
     CDS             complement(14755..15213)
                     /gene="asnC"
                     /locus_tag="ACHJWB_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4313236.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15365..16357
                     /gene="asnA"
                     /locus_tag="ACHJWB_001586"
     CDS             15365..16357
                     /gene="asnA"
                     /locus_tag="ACHJWB_001586"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4313237.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16362..17813)
                     /gene="viaA"
                     /locus_tag="ACHJWB_001587"
     CDS             complement(16362..17813)
                     /gene="viaA"
                     /locus_tag="ACHJWB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4313238.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17807..19303)
                     /gene="ravA"
                     /locus_tag="ACHJWB_001588"
     CDS             complement(17807..19303)
                     /gene="ravA"
                     /locus_tag="ACHJWB_001588"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4313239.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNEQPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19526..21394
                     /gene="kup"
                     /locus_tag="ACHJWB_001589"
     CDS             19526..21394
                     /gene="kup"
                     /locus_tag="ACHJWB_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4313240.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21561..21980
                     /gene="rbsD"
                     /locus_tag="ACHJWB_001590"
     CDS             21561..21980
                     /gene="rbsD"
                     /locus_tag="ACHJWB_001590"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4313241.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21988..23493
                     /gene="rbsA"
                     /locus_tag="ACHJWB_001591"
     CDS             21988..23493
                     /gene="rbsA"
                     /locus_tag="ACHJWB_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4313242.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23498..24463
                     /gene="rbsC"
                     /locus_tag="ACHJWB_001592"
     CDS             23498..24463
                     /gene="rbsC"
                     /locus_tag="ACHJWB_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4313243.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24488..25378
                     /gene="rbsB"
                     /locus_tag="ACHJWB_001593"
     CDS             24488..25378
                     /gene="rbsB"
                     /locus_tag="ACHJWB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4313244.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25504..26433
                     /gene="rbsK"
                     /locus_tag="ACHJWB_001594"
     CDS             25504..26433
                     /gene="rbsK"
                     /locus_tag="ACHJWB_001594"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4313245.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26437..27429
                     /gene="rbsR"
                     /locus_tag="ACHJWB_001595"
     CDS             26437..27429
                     /gene="rbsR"
                     /locus_tag="ACHJWB_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4313246.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27395..28822)
                     /gene="mdtD"
                     /locus_tag="ACHJWB_001596"
     CDS             complement(27395..28822)
                     /gene="mdtD"
                     /locus_tag="ACHJWB_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4313247.1"
                     /translation="MSDKKKRSMAGLPRIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28845..29537)
                     /gene="yieP"
                     /locus_tag="ACHJWB_001597"
     CDS             complement(28845..29537)
                     /gene="yieP"
                     /locus_tag="ACHJWB_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4313248.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VINHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7126 a         7599 c         7576 g         7349 t
ORIGIN      
        1 ttataaaaaa gggccaccgg tcggcagccc ttttttatct atgatagtga ttactcaacc
       61 gtaaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca
      121 tggtaagcca gcagctgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
      181 tgcggcatct cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgatcggcg
      241 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
      301 aacagttcgt tgttcggtgc aacgacgata accggcatat ccgcgtcaat cagcgccagc
      361 gggccgtgtt tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
      421 agcttcaatg cgccttccag cgcgattggg tactgatcgc cacggcccaa gaacagcgcg
      481 tgatgtttgt cagagaaatc ttctgccaga gcttcaatgc gtttgtcctg agacagcatc
      541 tgctcaatac ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca
      601 tccagacctt tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg
      661 gtgaatgctt tagttgatgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga
      721 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
      781 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
      841 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
      901 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtaacggga aaccataccg
      961 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg
     1021 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     1081 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata
     1141 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     1201 ccagttttat cgaagatgtt taccgaacgg cgagtaattt ccgcaatatc gccctcttca
     1261 aggaagataa agcgacgggt caccggcagc agcgccagct ggtcagaagc gataaagttt
     1321 tcgcccatac ccagaccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
     1381 gggtgacggg agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc
     1441 agaacagcct cacgcagagt accgccctgt ttcagctccc agttcaccag atgggcaatt
     1501 acttcggtgt ctgtttcaga aacgaaggta tagccacgcg cttttaactc ttcgcgcagc
     1561 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
     1621 tgcgcattca cttctgaagg ttcaccgtgg gtcgcccagc gagtatgagc aatacccgta
     1681 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga
     1741 cgcaggcggg tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg
     1801 cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
     1861 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     1921 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     1981 gcttgtggga gggagattat gttatctcct catcccagat gaccggatta cccggccaga
     2041 attacttttt ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga
     2101 tagctaatgc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
     2161 ctttgcctac tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc
     2221 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
     2281 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
     2341 ctttacccag acgcgctttt ttcatctcaa caaagttacc gacgtgtgca ccttccagca
     2401 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg
     2461 cgtcttccac aacggtatac ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
     2521 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     2581 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg
     2641 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     2701 cgcgctccag acgggagagt tgcaggcggt tattcacacc ttctacttcg cttaaacgtt
     2761 gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga
     2821 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     2881 cgccgttagc gatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     2941 catctttgtg ctcaacaatg ccggtaactt tgccattttc acgggtgata cgtccataac
     3001 cggtcggatc atccagtttc accgtcagca gaccaatgcc accctgcggt ttagcatcac
     3061 gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     3121 cttcatcatc ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta cccagctgct
     3181 ctgcctgaag cacccagtta aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc
     3241 cgtgaccgta caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     3301 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     3361 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     3421 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     3481 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagtgc
     3541 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     3601 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     3661 ttttatatca gcaggatcta tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc
     3721 cagcctgttt ccagactggc ttttgtgctt ttcaagccgg tgttacatcg cttttttggt
     3781 caactcgata acgcgcagct gcgcgatcgc tttggccagt tccgcagacg cctgagcgta
     3841 atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc
     3901 tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc
     3961 aggctgcact tcaagaatgc cgccagacag atagataaac tcttcgtgac cgtgctgttt
     4021 cacgatgcga atcataccag gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat
     4081 ccccagttca ccttcgctac ccgttacctg gattttctcg accagaccag agaacatttg
     4141 ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc atatcaccct ccgattaagg
     4201 cgttaaagtt ttttggcttt ttccacagct tcttcgatgg aaccgaccat gtagaacgcc
     4261 tgctccggca ggtgatcgta ttcgccttcc atgatgcctt taaagccacg gatggtgtct
     4321 ttcagggaga cgtatttacc cggagaaccg gtgaatactt ctgccacgaa gaacggctgg
     4381 gacaggaagc gctggatctt acgagcacgc gctaccacca gtttgtcttc ttcagacagt
     4441 tcatccatac ccaggatggc gatgatgtct ttcagttcct gataacgttg caggatggac
     4501 tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg
     4561 ctggtggagt ccagcgggtc aacggccggg tagataccca gagacgcgat ctgacggctc
     4621 agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca
     4681 tccgcaggta cgtataccgc ctgtacggag gtgatagaac cagttttggt ggaggtgata
     4741 cgttcctgca gaacgcccat ctcttccgcc agggtcggct gataacctac cgctgaaggc
     4801 atacggccca gcagtgcgga tacttccgta ccggccaggg tgtaacgata gatgttgtca
     4861 acgaacagca gaacgtcacg accttcgtca cggaatttct cagccatggt cagaccggtc
     4921 agagcaacgc gcagacggtt tcccggcggc tcgttcatct ggccatacac cagggatact
     4981 ttgtcgataa cgttggagtc ggtcatttcg tggtagaagt cgttaccctc acgagtacgt
     5041 tcacctacgc ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc
     5101 tccatcatgt ttacggtttt acctacaccc gcaccaccga acagaccaac tttaccgccc
     5161 ttagcgaacg gacacatcag gtcgataact ttgataccgg tttccagcag ttcctgagag
     5221 tttgacagct cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg
     5281 atctcgcctt tcatgtcgac cggttcaccc agtacgttca tgatacggcc cagagtcgct
     5341 ttacctaccg ggacttcaat cgggtgttcg aggtctttta catccagacc gcgacgcaga
     5401 ccgtcggagg aacccattgc gatggtacgt acgataccgc cgccgagctg ctgctgaact
     5461 tccagcacca gacgctcatt accattttgc acctcaagag catcgtacac gcgcggtacg
     5521 gcatcctgag ggaattcgac gtcaactacg gcgccgatta cctggacaat ctttccagta
     5581 gccatcttaa atcctctacg aaataacctg tttaaaccgc ggcggccccc gagacgatct
     5641 cggtgagttc ctgagtaatg ctggcctgac gagctttgtt gtataccaac tgcagctctt
     5701 taatcaggct gccgccattg tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct
     5761 cgctggccag gttttcaacc acgccctgat aaacctgaga ttcgacataa cgacgcagca
     5821 gggtatccag caacgccttc ggatcgggtt cgtacaggta atcccaggat ttatgtttca
     5881 gatcatcatc atctgatgcc ggtaacggca gcagctggct gatggtcgga acctgagaca
     5941 tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg
     6001 cctgcaacat cacttttacc ggaccgatca gttcggacag ggaagggtta tcccccatgc
     6061 cggtgacctg ggcaacaaca ttgccgccca cggagttgaa gaacgacacg cctttcgagc
     6121 cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca
     6181 gttttttgaa caggttaatg ttcaaaccac cgcacaaacc acggtcggtc gacaccacca
     6241 ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg gtgcttatat tccagattac
     6301 cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc ataaggacgg ctggccgcca
     6361 tgcgatcctg cgatttacgc attttggaag cggcgaccat ctccatcgct ttagtgatct
     6421 tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc ttttgcgccg gccatgagct
     6481 tctcctcaat gccttgcggc ctgccctaag gcaagccgcc agacgttacc aggattgggt
     6541 tgctttgaag gaatcgagga tgcctttcag cttgccttcg atttcgtcgt tgtagccacc
     6601 ggtctggttg atctcttgca tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc
     6661 ggcttcgaag ctgccaattt tcgacagttc aacatccgcc aggtaaccac gttctgctgc
     6721 gaacagaacc agagactgct gcgcaacgga catcggcgca tactgtttct gtttcagcag
     6781 ttcggtcact ttctgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc
     6841 aaactgagag aacgctgcca gttcacgata ctgtgccaga gcggtacgga taccaccgga
     6901 cagttttttc atgatcttgg tctgtgctgc accaccaaca cgggatacgg aaatacccgg
     6961 gttaaccgca ggacgaatac cggcgttgaa caggttggtt tccaggaaga tctgaccatc
     7021 ggtaatggag attacgttgg tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat
     7081 aatcggcagt gcggtcagag aaccggtttt ccctttcact tcacctttgg tgaaggcttc
     7141 aacgtattcg gcgttaacac gtgcagcacg ctccagcaga cgagagtgga gatagaaaac
     7201 gtcgcccggg aatgcttcac gtcctggcgg acgacggagc agcagggaga tctgacggta
     7261 agcaacagcc tgtttagaca ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg
     7321 gaagtattcg cccattgcgc aaccggcata cggtgccagg tattgcagtg cagcggattc
     7381 agacgcggtt gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg
     7441 taccacgtta gaaatggtgg acgctttctg gccgatagcg acatagatac atttgatacc
     7501 ggaatcgcgc tggttgatga tggcatcgat agccagtgcg gttttacctg tctgacggtc
     7561 accgatgatc aattcacgct gaccacgacc gattgggatc atggagtcaa cggctttata
     7621 accggtctgt accggctgat ctacggactg acgttcgata acgcccggag cgattgcttc
     7681 tacagcagag aagccgtcgt gatccagcgg acctttaccg tcgattggtg cacccagagt
     7741 gttaaccaca cggcccagca ggccacggcc aaccggaact tccaggatac ggccagtaca
     7801 cttaactttc atgccttcgg caaggtcagc gtacggaccc ataacaaccg cacctacaga
     7861 gtcgcgctcg aggttcagtg cgatagcgta acggttaccc ggcagggaga tcatttcacc
     7921 ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg tcacttacag aaacaatagt
     7981 accttcgttg tgagcttcac tcacaacatt gaactgagca atgcgctgct tgatcagttc
     8041 gctgatttcg gtggaattca gttgcatgct ccagtcccct taagactgca agacgtctgc
     8101 aaggcgctca agacgaccgc gtacgctgcc atcaatgacc atatcacccg ctcggatgat
     8161 aacgcctgcc attacagact tatcgatttt gcaattcagc ttaactttgc gtgacagacg
     8221 tttttccatc gcagcagaaa ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat
     8281 gacgtctacc tcagcggtag cctcactcac ggcacgaagg tgaataaact gctccagaac
     8341 atccgggagc gcgttaagac gaccattttc agccattacc cgaatcaggt tctgaccgtt
     8401 ttcgtccagt tgctcaccac aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag
     8461 cgcgccagag agaagctctg ccatttgttc gtttttggtt acctcggcgg caaacgccag
     8521 catgtcctgc cagcgttcta cactttggtg ttcgacggca aagtcaaaag ctgctttggc
     8581 gtaggggcga gctaccgtaa taaattcaga catcagcccc tccctcctta cagttcagcg
     8641 acaagtttat ccacgatgtc gctgttagca gcttcatcca cggaacgttc gatgatcttc
     8701 tcggcgccag caacagccag gatagcaact tgcttacgca gctcttcacg ggcacgttta
     8761 cgctcggctt caatttctgc ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc
     8821 tcagctttcg cttcgtccag aatctgcgag cggcgtttgt tcgcctgctc gatgattacc
     8881 tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg
     8941 tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg
     9001 atggctgcca ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa caggacaaac
     9061 gcgatggcct ggccgaggat tgttgcgtta agattcacag cacaatgcct ctatttagtt
     9121 aacgttctga tattgctctt taaataaaag caacgcttac tacgcgacag cgaacatcac
     9181 gtacagaccc agacctacag cgatcatcgg gatagcatcc accagaccca taacgataaa
     9241 gaactgagta cgcagcagag gaatcagatc aggttgacgc gctgcgcctt ccaggaattt
     9301 acccccgagg atgccgatac cgatcgcagc accgattgcc gccagaccca tcatcacagc
     9361 ggcagccatg tacagcagat ccatattcag gttttccatg acagtctcca gtttgtttca
     9421 gttaaaacgt agtagtgttg gtaaattaat gttcttcaga cgccatcgac agatagacga
     9481 tcgtcagaac catgaagatg aaggcttgca gcgtaatgat caggatgtgg aaaatggccc
     9541 acggcacatt caggatccac tgtgaccacc acggcaacag accagcaatc agaatgaaaa
     9601 tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc aagtgaaact ggtttggaca
     9661 gcaggcttac cccttcaagg attaagttga caggaatgaa cgcccagtga ttgaacggct
     9721 gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat tttgatgctg tagaacagaa
     9781 tcaggataaa tacgcccaat gccatagaca gcgttacgtt cacgtccgca gacggaacca
     9841 cacgcagtgc aggcagaccc agtacatgtt cagcaatgta cggcagcagg tcgataggca
     9901 gtaaatccat caggttcatc aggaataccc agacgaagat cgtcagggcc agcggagcaa
     9961 tcagcttgct tttgccatgg tacatgtctt tcacgctacc attaacaaag ccgatcacca
    10021 gctcaatcgc ggtctgaaac ttacctggca caccgctggt cgcctttttg gctacgctac
    10081 ggaataaaac caggaacaac agacccagca ccaccgagaa gaacatggag tcaatattga
    10141 ttgtccagaa ggtggctggg gggttttgtg gatccaccag cgagaatgta cgcaggtcca
    10201 gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca
    10261 tgatgccttt taccctttgt tgttaattac agccggtgcc agtatctgaa ccaccagcac
    10321 caaaacccac gtaacgatca gcggcaagaa taccgccttt aaaaccgcca acgccaccac
    10381 cagtaacacc aacatcgcca gaactttgaa agcttcgcca aatgcgaatg tccaggccac
    10441 ccggcctttc gctggtgtat gcgcctggtg acgccaggca aatatcataa acaaaacgtt
    10501 aggcagaaag actgccaggc ccccgcttat tgcagagacg ccccagaagg ggtctttgag
    10561 gctgaacagc aatccacttg ctatcaccac cagtaactga acgagcagaa gcttccgagc
    10621 aacgtttcga ctcacgagcg acacagacat cacgtttttc actcctgctc ccttcgaggt
    10681 atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt caagcatcaa aaagcggtca
    10741 aattatacgg tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg aataaatgtt
    10801 taaatatttt tccagtgcat acaattgcga cttttctgct aaccctgttc gatcatgaaa
    10861 aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca tattgcgcat
    10921 gttcgcgcac agcatattta tttacttggc aaatgatgcc tttgcaagtt tatgatattt
    10981 cagtctaaaa acagatactg ttttaataaa tgacatttac acaacaaaaa ccacccattg
    11041 acatttttaa taatgtttta acagccaatg atggttctta gcgccgattt ttagcagact
    11101 gatattttca ctaatgactt attttctgct taccaaaaaa agccacgtta tcttgttgat
    11161 gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt
    11221 gttaacagtc taaccggtca attttttatg atttttttga taaaaattaa attttatttg
    11281 ctttaatcac caccagatga cgttcgccat ccagggctgg aacctgaagt ttaaccactg
    11341 attcgacctg atattcttcg ggcaacaaag cgatttcatc ttccggcatt tgccctttca
    11401 gcgcgtaaaa acggccttgc tcaccaggaa gatggtggca ccagctcacc atatcgttca
    11461 gagaggcaaa agcgcggcta attacgccat caaatggcgg ctctgaagga aactcttcta
    11521 ccctgctctg tactggttca atattctcca gtttaagctc atgttgcacc tgacgaagga
    11581 aacgcacgcg tttaccaagg ctatccaaca gagtgaaatg ggcttcagga cgcacgatag
    11641 agagtggaat gcctggcagt cctggtccgg tgccgacatc gataaaccgt tcaccttgca
    11701 gatacggtgc caccacaatg ctatcgagaa tatggcgtac cagcatctca ttaggatcgc
    11761 ggaccgaagt caggttgtac gctttgttcc atttatgcag catattcacg taggcaataa
    11821 gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt
    11881 tgttgagcac ggtgattacc tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg
    11941 catttttaat gcgttatgcg ctacgacgca gcataccctg ttttttcagc cacaccagca
    12001 gaatggagat ggccgcaggc gtgacgccag aaatacgcga agcttggccg atagaggctg
    12061 gtttgtgatc gttaagtttg gcgatcactt cgttagaaag accggatacc tggcggtaat
    12121 ccagtgtcgc gggtagcagg gtgttctcgt tacgcagctg cttttcgatc tcatcttgct
    12181 ggcgcgcgat ataaccttcg tatttaacct gaatctcaac ctgttccgcc gcctgttcgt
    12241 ctgtcaacgc aggggcaaac ggcgtcagcg tggttaattt ttcataagtc atttccggac
    12301 gacgcagcag atcttcacca ctggcttcac gggaaagcgg cgcagtcagg tgagcattca
    12361 cttcggctgc agcttccgcc gacggggtta cccaggtcga tttcagacgc tgacgctcac
    12421 gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca
    12481 gttcacgacc gatttcagtc aaacgcagat ccgcattatc ttcgcgtagc atcagacgat
    12541 attctgcgcg cgaagtaaac atacgatacg gttctttggt tcctaaagtg cacaggtcat
    12601 caactagtac gccgagatac gcctgagaac gtgccggagc ccaaccttct ttgtcagcag
    12661 acagacgggc agcgttaaga ccggccagca aaccttgcgc agcggcttct tcgtaaccgg
    12721 tagtgccgtt aatctgacca gcaaagaaca gcccctggat aaacttgctc tccagcgtcg
    12781 gtttcaggtc gcgaggatcg aagaagtcat actcaatggc ataacccgga cgcacgatct
    12841 tcgcgttttc catcccctgc atagagcgga cgatttgcat ctgcacatcg aacggcaggc
    12901 tggtggagat accgttcgga taaatttcat tagaggtcag tccttccggt tcaaggaaga
    12961 tctgatgctg atttctgtcg gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc
    13021 gtgggccgac accttcgatc acccctgcat acattgggct acgatcgagg ttactgcgga
    13081 tcacatcatg ggttttctcg ttggtatgag tgatataaca cggcacctgc tggggatgct
    13141 gggacgcatt gcccataaac gagaataccg gcattgggtt atcgccatgc tgttgcgcca
    13201 gcacactaaa gtcgatagtt cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac
    13261 caacgcgcag cggcagttca cgcaaacggc gagaaagcgg aatggacggc ggatcaccag
    13321 cacggccacc gctgtaatta tccagaccga tatgaatttt accgtcgagg aacgtcccaa
    13381 cggtgagcac gacggctttg gcacggaact tcagtcccat ttgggtaaca gcaccgacca
    13441 cgcgatcgtt ttcgacaata agatcttcaa ccgcctgctg gaagatcatc aggttcggtt
    13501 ggttctccag cgccgtacgt accgcctgac ggtagagcac acgatccgcc tgagctcggg
    13561 tagcgcgaac cgccggtcct ttgcttgcgt ttagtatcct aaactggata cccgcttgat
    13621 cgatcgcttt cgccatcaga ccgccgagtg catccacttc ttttaccaga tgtcccttcc
    13681 caataccgcc gatcgccggg ttgcagctca tctgccccag agtgtcgata ttgtgtgtca
    13741 aaagcagagt ctgttgaccc atacgcgccg cggccatcgc ggcctcggtg cctgcatgac
    13801 ccccgccaat gatgatgacg tcaaaaggat ccggataaaa catggtgatt gcctcgcata
    13861 acgcggtatg aaaatggatt gaagcccggg ccgtggattc tactcaactt tgtcggcttg
    13921 agaaagacct gggatcctgg gtattaaaaa gaagatctat ttatttagag atctgttcta
    13981 ttgtgatctc ttattaggat cgcactgccc tgtggataac aaggatccgg cttttaagat
    14041 caacaacctg gaaaggatca ttaactgtga atgatcggtg atcctggacc gtataagctg
    14101 ggatcagaat gaggggttat acacaactca aaaactgaac aaccgttgtt ccttggataa
    14161 ctaccggttg atccaagctt cctgacagag ttatccacag tagatcgcac gatctgtata
    14221 cttattagag taaattaatc cacgatccca gccattcttc tgccggatct tccggaatgt
    14281 cgtgatcaag aatgttgatc ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga
    14341 gttctgcctc gagtttatcg atagccccac aaaaggtgtc atattcacga ctaccaatac
    14401 cgattgcgcc aaagcggacc gcagaaagat cgggcttctg ttcctgcaat gcttcataga
    14461 aaggagaaag gttgtccgga atatctccgg caccgtgggt ggagctgata atcagccaga
    14521 tccccgaggc agataaatct tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg
    14581 cctcttccag cttttcagcc aggtgttctg ctacatattc ggcaccgccg agggtgctgc
    14641 cgctgataag agtgatatct gccataaacc gccaccttta ttaagagtgg cgtattgtac
    14701 gctgtgaacg cgttgggatc tacctgtgga aaagtatggg attaaaaaag ccgatcaggg
    14761 cttgatagta cgcatgatcg ggttttgcag gacgatcaat gtctcggtgg actgaatttc
    14821 atcaattgtt tggatcttgt tgataagtac atgctggaga gcgtcgatcg aacggcacat
    14881 tacttttata aagatgctgt agtggcctgt tgtgtaatag gcttcagtga cttcatcaag
    14941 gctttccagc tttgccagcg cggaagggta gtctttggcg ctctttaata taatgccgat
    15001 aaagcagcct acgtcataac cgagctgctt cgggctgaca tcaatacgcg ccccggtaat
    15061 gatccccgcc tgcttcattt tctctactcg aacgtgaatc gtccccggac tgacgccaaa
    15121 ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc attaatgctt ccaggatgcc
    15181 acggtccaga ttgtcgatca gataattttc cataggattt tcttatgcag attgatgatt
    15241 cattctattt tagccttctt ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg
    15301 attattgcat gtgtgtcggt ttttgttgct taatcataag caacaggacg caggagtata
    15361 aaaaatgaaa accgcttaca ttgccaaaca acgccaaatt agcttcgtga aatctcactt
    15421 ttctcgccaa ctggaagaac gtctggggct gatcgaagtc caggcgccga ttcttagccg
    15481 tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt
    15541 gaaagctctg cctgatgccc agttcgaagt ggttcattca ctggcgaagt ggaaacgtca
    15601 gaccttaggg caacacgact tcagcgcggg cgaagggctg tacacgcaca tgaaagccct
    15661 tcgccccgat gaagagcgtc tttctccgtt gcactcggtc tatgttgacc agtgggactg
    15721 ggaacgcgta atgggcgacg gtgagcgtca attctcgact ctgaaaagca cggtagaggc
    15781 gatctgggcg ggaattaaag caaccgaagc tgcagttagc gaagagtttg gtttggcacc
    15841 gttcctgccg gatcagatcc acttcgtaca cagccaggag ttactgtctc gttatccgga
    15901 tctcgatgcc aaagggcgtg agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt
    15961 cgggattggc ggcaagctga gtgatggtca tcgtcacgac gtgcgcgcac cggattatga
    16021 tgactggagc accccgtcag agctgggcca tgcgggtctg aacggcgata ttctggtatg
    16081 gaaccctgta ctggaagatg cgtttgagct ttcttccatg gggatccgcg tggatgccga
    16141 cacgttgaag catcagttgg cgctgaccgg tgatgaagat cgtctgcagc tggaatggca
    16201 tcaggcgctg ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg gccagtctcg
    16261 tttgaccatg ctgctgctgc aactgccgca tatcggccag gttcagtgtg gagtatggcc
    16321 agctgctgtt cgcgagagcg tcccttctct gctgtaataa tttatcgccg ccagcgtctg
    16381 agcaggcggc ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg
    16441 ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc gatgctgatg tacccgctgc
    16501 agctctttca ctttactcgt cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc
    16561 accaccgcat cggcatcaaa ccattccctg ctttgcaagc gttccataat ggcgcgaaaa
    16621 caactggcaa gatcggtgcc gccacgaaac tgctggctta aaaaacggat tgcttgttcg
    16681 atgccttgtg ggcctgaaag ctcataacgg acgatctcgg tggaaaatag cataatatag
    16741 cagcgtcggt tttctgcgag agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac
    16801 tgttcattaa agccgcccat tgagccggaa gtatccacac agacaataaa cggcccgcgc
    16861 ggctgttcat cgtaatcttt atgtaccacc ggacgttcga tcactttttc acgccacgac
    16921 tcaccgtgca ggcgataggt gagcaactgt ttttccacca gccgacggta aaactcatac
    16981 tccagttccg ttatccctag tgtcgccagt tctggcggca ggagacgtaa aatatcatcg
    17041 ctttgttgca gaccatcaac ctgctcagga accgtcgccg gttcgcgcac catggtgcgg
    17101 aaggtttcca tctgcgcatc gttgcgcggt attgatttgg cttcccgaga acgccccagc
    17161 tgctctgcca ggcgtttcag ttccggctgt tcgttaagaa attcaccgta tttcacaatc
    17221 aactgatagt cgccacgttt aagctggccg gcgctcatat cccacagacg accagctgcg
    17281 gtattgttat ctgcgagaat cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact
    17341 tcacttaaca gttgttcgcg ttcttcttct aatagctgtt gatttaacgt cgttgcttgc
    17401 acgatcagac ttaaacgcca gcgctggaga aaaagcgtgt gtaacgctga agtgatcgtg
    17461 ctgttagcat caaccaactg tcgggcttgt tctgcccatg gagaattcag acgatgcagt
    17521 aagtccagga tctgtggtag ctgcacaata aactgcggcg tggagaggag ctggctttgc
    17581 tgatagcaca tcacctcttc ggtgagttcc ggcgggactc gggcatcttt cagccgactg
    17641 cgcagtgcct cacgccagcg gggaacatca tcagtgattg ccgccttcag tcgtgggaat
    17701 ttctcaaaga agactgccag ctgcggtgag gccagcagcg cgatgatcat ctcttcgatc
    17761 aatccctctt cgctgacggc cagcatcaca ttaagcgtat ccagcgttag cattgttgtg
    17821 cctggcgaat ctgtgcaccg acatcctgta ggctggcttc aatgcgacct aaccagtcgc
    17881 cagggataaa cagacatttt tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc
    17941 tttcaagctc ttccagttgt tgttttattt caccaggcag accttcagcc gatgaaccgg
    18001 gaagtgccag cgtactgcct tgtaaactta catcgcgaac gacaagatgt tgggcgctat
    18061 caacttccag attcagtttc tgggcaaagc cgataccgtt cagtttaccg cgaatttcac
    18121 cacctttgct cagccactgt tccagcgcgc tacgctcaaa ggagatatga accacttcca
    18181 tatcatgcag ttttaacggt ttttgcagca gtagagtcag agtggaagca gtaacgttaa
    18241 caggaagttg atactgctga cgacggctga aaatgccgcc cagacgaatt accgttaagg
    18301 ctgttttatc gctttgttgc tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc
    18361 gagtcaacat cccttgctgt tgccaggcgt gaccggtcat caatacatca atttgttgtt
    18421 gtatcaaatt caggctttgc gcgtcatacc acaggcaatc tttcagcaaa atgagatcta
    18481 ccggggcaac agcactgcga ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt
    18541 ttttccagcg acgatccgag acataaggcg catccggtaa tttatccagt tgctggcgca
    18601 gcataaaaat cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc
    18661 aacgttcata ttcttcatct gtgacctgca aggcatcagg aaccggattg tcgttttcat
    18721 cctgttgact ggtcagcatg gagcggaaat tcgctttatc ctgcacttta tctaaccaca
    18781 gacgaatcag catgcggtca tataacgctt ccagactgct gtctgcttcc ggctgctcgt
    18841 tggaggccgc caccagcaga cgcatcggga ttttttctac gtgtgcaccg ttgcggaact
    18901 ggcgctcgtt aatggcggtg agcaaggtat taagaattgc cgggcccgct ttccagatct
    18961 catccagaaa gacaatttct gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc
    19021 cttcatcttt tagcgcctga atagaaaggg gaccaaaaac ttcttccggc gtggagaagc
    19081 gggtcatcag atattcaaac gcgcgggcat tctgaaaggc gaattttaag cgccgggcga
    19141 tcaaactttt ggcaatacct ggcgggccaa ggaggaacac actttcacca cttaatgccg
    19201 ctaataaaca caagcggatg gcgtggctac gttcataaag ccccttttcc aacgaactgc
    19261 tcaggcggga aattctttcc gctaataaat gagggtgagc cataatgaag tggcgtcctt
    19321 tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg ctaaaatagt
    19381 ctcaaagggg gggtattttt ctttgagcca ggttaatgtg gccgcattta ggagtacgat
    19441 tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc caagggacta agcacacatt
    19501 tcatatttca acgaaagact agtctatgag cactgataat aagcaatcat tgcccgcgat
    19561 taccctcgcg gcgattggag ttgtctacgg cgatattggt accagcccgt tatatacact
    19621 tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa cgcgatgccg tgtttggctt
    19681 tttatcgctg atcttctggc tgctaatctt tgtggtttcc attaaatatc tcaccttcgt
    19741 gatgcgggca gataacgccg gtgagggggg gatcctgacg ttgatgtcgc ttgccgggcg
    19801 taatacgtcg gcgcgaacca catcaatgct ggtgattatg gggctaatcg gcggcagctt
    19861 tttctatggt gaagtcgtca taacacccgc tatttcggtg atgtcagcca ttgaaggtct
    19921 ggaaatcgtc gccccgcagc tggatacctg gatagttccc ctctcaatta tcgttctcac
    19981 tttattattt atgattcaaa aacatggcac ggctatggtc ggtaagctgt ttgcgccgat
    20041 catgctgacc tggtttttga ttctggcagg actggggtta cgtagcatta ttgctaaccc
    20101 ggaagtgctg catgcgctga atccaatgtg ggcggtgcat ttcttccttg aatacaaaac
    20161 ggtttctttt attgcattag gggcagtggt gttgtcgatt acgggggtcg aggcgctgta
    20221 tgctgatatg gggcactttg gtaagttccc tattcgcctg gcgtggttca ccgtcgtatt
    20281 accttccttg acccttaatt acttcggtca gggagcgctg ttgttaaaga acccggaagc
    20341 gattaagaac ccgttcttcc tgttggcacc ggactgggcg ctgatcccgc tgctgatcat
    20401 cgccgcactg gcgacggtaa ttgcctcgca ggcggttatc tctggcgtct tctcattgac
    20461 gcgtcaggcg gtacgtctgg gatatttatc gccgatgcgc attattcaca cctccgaaat
    20521 ggagtcaggg caaatctata ttccgtttgt gaactggatg ctctatgtcg cggtcgtgat
    20581 tgtgattgtc agctttgagc actccagcaa cctggcggcg gcgtacggga ttgcggtgac
    20641 cggaaccatg gtgctgacgt ctattctctc gactaccgtg gcacgtcaga actggcactg
    20701 gaataagtat tttgttgcgc tgatcctgat tgctttcctt tgtgtcgata ttccattgtt
    20761 caccgctaac ctcgataaac tgctctccgg cggctggttg ccattgagcc tcggtacggt
    20821 gatgtttatc gtgatgacca cctggaagag cgagcgtttc cgcttgctgc ggcggatgca
    20881 tgaacatggt aactctctgg aagcgatgat tgcttcgctg gagaaatcac cgcctgttcg
    20941 cgtgcccggg accgcggtgt atatgtcgcg tgcaatcaac gtcattccct ttgcgctgat
    21001 gcataacctt aaacataaca aggtattgca tgagcgggtg attctgttaa ctctgcgtac
    21061 cgaagacgcg ccatatgtcc ataatgtccg tcgggtacag attgaacaac tgtcgcccac
    21121 tttctggcgc gtggtggcaa gttatggttg gcgagaaacg ccaaacgtgg aagaagtttt
    21181 ccaccgctgc ggtctggaag gattaagttg ccggatgatg gaaacctcct tctttatgtc
    21241 gcatgagtcg ttgatcctcg gcaaacgccc gtggtatttg cgtctgcgcg gcaagctgta
    21301 cttgctgctg caacgtaatg cgctgcgtgc accagatcaa tttgaaatcc cgccaaacag
    21361 ggttatcgaa ctgggtactc aggtcgaaat ctaacgccag acgcctcctt tctccataag
    21421 ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg tttcgaggtt gatcacattt
    21481 ccgtaacgtc acgatggttt tcccaactca gtcaggatta aactgtgggt cagcgaaacg
    21541 tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct taattctgat atttcatcgg
    21601 tgatctcccg tctgggacat accgatacgc tggtggtgtg tgatgctggt ttacccatct
    21661 ccaaaagtac aacgcgtatc gatatggcat taacccaggg tgtaccttct tttatgcagg
    21721 tgctgggcgt cgttacaaat gaaatgcagg tcgaggctgc cattatcgcg gaagagatca
    21781 aacaacataa tccgcaactc cacgaaacgt tgctcactca ccttgagcag ctgcaaaaac
    21841 accagggaaa taccattgaa attcgttaca ccacgcatga acaattcaaa caacaaaccg
    21901 cagaaagtca ggcggtaatt cgcagcggag aatgttctcc gtatgcgaat atcattctct
    21961 gtgctggcgt gacgttctga ggccgtcatg gaagcattac ttcagcttaa aggcatcgat
    22021 aaagccttcc cgggcgtaaa agccctctcg ggcgcagcgt taaatgtcta tccgggccgc
    22081 gtgatggcgc tggtgggcga aaacggcgcg ggtaaatcca ccatgatgaa agtgcttact
    22141 ggtatctatg ctcgcgatgc cggcacgctt ttatggctgg ggaaagaaac gacatttacc
    22201 gggccgaaat cttcccagga agccgggatt gggattatcc atcaggaact gaacctgatc
    22261 ccgcagttga ccattgccga aaacattttc ctcggtcgtg agtttgttaa tcgctttggc
    22321 aaaattgact ggaaaaccat gtatgccgaa gcggataaat tgctggctaa acttaacctg
    22381 cgctttaaaa gcgacaagct ggtgggcgat ctttccatcg gtgaccagca aatggttgaa
    22441 atcgccaaag tgctgagctt tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg
    22501 ctgaccgata ccgaaaccga atccctgttc cgcgtcatcc gcgaactaaa atcgcaaggc
    22561 cgcggtattg tctatatctc ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt
    22621 accgtttttc gtgacgggca atttattgct gagcgcgaag tggcatcact gaccgaagat
    22681 tcgctgattg agatgatggt gggtcgcaag ctggaagatc aatatccgca cctggacaaa
    22741 gcgccgggag atatccgcct gaaagtcgat aatctctgcg gacttggcgt taacgatgtc
    22801 tcttttactt tacgcaaagg cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt
    22861 accgaactga tgaaagtgct ctacggcgca ctaccgcgca ccagcggtta cgtcaccctg
    22921 gatgggcatg aagtcgttac ccgttcaccg caggatggcc tggcaaacgg cattgtgtat
    22981 atctccgaag accgtaaacg tgacggttta gtgttgggca tgtcagtaaa agagaacatg
    23041 tcgctgacag cgctgcgcta cttcagccgc gctggcggca gtttgaagca tgccgatgaa
    23101 cagcaggctg tgagtgattt cattcgtctg tttaatgtga aaacaccgtc gatggaacag
    23161 gcaattggtc tgctttccgg tggcaatcag caaaaagtgg cgattgcccg cggtctgatg
    23221 acacgcccca aagtgttgat ccttgatgaa cctacccgtg gcgtagatgt cggcgcgaaa
    23281 aaagagatct atcaactgat taaccagttc aaagccgatg gcttgagcat cattctggtg
    23341 tcatcggaga tgccagaagt attaggcatg agcgatcgca tcatcgtcat gcatgaaggg
    23401 catctcagcg gggaatttac tcgtgagcag gccacccagg aagtgttaat ggctgccgct
    23461 gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg acaacccaga ctgtctctgg
    23521 tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt
    23581 gctgatcgcg attgtctcga cgttaagccc gaactttttc accatcaata acttattcaa
    23641 tattcttcag caaacctcgg tgaacgccat tatggcggtc gggatgacgc tggtgatcct
    23701 gacgtcgggt atcgacttgt cggtaggttc tctgttggcg ctgaccggcg cagttgctgc
    23761 atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc gctgccgctc tcgcgttagg
    23821 tgccgcaatt ggtgcggtaa ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat
    23881 cgctacgctg gttatgatgc ttttactgcg cggcgtgacc atggtttata ccaacggtag
    23941 cccagtgaat accggcttta ctgagaacgc cgatctgttt ggctggtttg gtattggtcg
    24001 tccgctgggc gtaccgacgc cagtctggat catggggatt gtcttcctcg cggcctggta
    24061 catgctgcat cacacgcgtc tggggcgtta catctacgcg ctgggcggca acgaagcggc
    24121 aacgcgtctt tctggtatca acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg
    24181 tctgctggca tcgctggccg ggatcattga agtggcgcgt ctctcctccg cacaacccac
    24241 ggcggggact ggctatgagc tggatgctat tgctgcggtg gttctgggcg gtacgagtct
    24301 ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc gcattaattc ttggcttcct
    24361 taataatgga ttgaatttgt taggtgtttc ctcctattac cagatgatcg tcaaagcggt
    24421 ggtgattttg ctggcggtgc tggtagacaa caaaaagcag taataacgac tacaggacat
    24481 cttgaatatg aacatgaaaa aactggctac cctggtttcc gctgttgcgc taagcgccac
    24541 cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg gtggtctcca cgcttaacaa
    24601 cccgttcttt gtatcgctga aagatggcgc gcagaaagag gcggataagc ttggctataa
    24661 cctggtggtg ctggactccc agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt
    24721 aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc gactccgacg cagtaggtaa
    24781 tgctgtgaag atggctaacc aggcgaacat cccggttatc actcttgacc gccaggcaac
    24841 gaaaggtgaa gtggtgagcc acattgcttc tgataacgta ctgggtggca aaatcgccgg
    24901 tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc
    24961 tggtacatcc gcagcccgtg aacgtggcga aggcttccag caggccgttg ctgctcacaa
    25021 gtttaatgtt cttgccagcc agccagcaga ttttgatcgc actaaaggtt tgaacgtaat
    25081 gcagaacctg ttgaccgctc atccggatgt tcaggctgta ttcgcgcaga atgatgaaat
    25141 ggcgctgggc gcgctgcgcg cactgcaaac tgccggtaaa tcggatgtga tggtcgtcgg
    25201 atttgacggt acaccggatg gcgaaaaagc ggtgaatgat ggcaaactag cagcgactat
    25261 cgctcagcta cccgatcaga ttggcgcgaa aggcgtcgaa accgcagata aagtgctgaa
    25321 aggcgagaaa gttcaggcta agtatccggt tgatctgaaa ctggttgtta agcagtagtt
    25381 ttaatcaggt tgtatgacct gatggtgaca taaatacgtc atcgacagat gaacgtgtaa
    25441 tataaagaaa agcagggcac gcgccaccct aacacggtgg cgcattttat ggacatcccg
    25501 aatatgcaaa acgcaggcag cctcgttgtt cttggcagca ttaatgctga ccacattctt
    25561 aatcttcaat cttttcctac tccaggcgaa accgtaaccg gtaaccacta tcaggttgca
    25621 tttggcggca aaggcgcgaa tcaggctgtg gctgctgggc gtagcggtgc gaatatcgcg
    25681 tttattgcct gtacgggtga tgacagcatt ggtgagagcg ttcgccagca gctcgccact
    25741 gataacattg atattactcc ggtcagcgtg atcaaaggcg aatcaacagg tgtggcgctg
    25801 atttttgtta atggcgaagg tgagaatgtc atcggtattc atgccggcgc taatgctgcc
    25861 ctttccccgg cgctggtgga agcgcaacgt gagcgtattg ccaacgcgtc agcattatta
    25921 atgcagctgg aatcaccact cgaaagtgtg atggcagcgg cgaaaatcgc ccatcaaaat
    25981 aagactatcg ttgcgcttaa cccggctccg gctcgcgaac ttcctgacga actgctggcg
    26041 ctggtggaca ttattacgcc aaacgaaacg gaagcagaaa agctcaccgg tattcgtgtt
    26101 gaaaatgatg aagatgcagc gaaggcggcg caggtactgc atgaaaaagg tatccgtact
    26161 gtactgatta ctttaggaag tcgtggtgta tgggctagcg tgaatggtga aggtcagcgc
    26221 gttcctggat tccgggtgca ggctgtcgat accattgctg ccggagatac ctttaacggc
    26281 gcgttaatca cggcgttgct ggaagaaaaa ccattgccag aggcgattcg gtttgcccat
    26341 gctgccgctg cgattgccgt aacacgtaaa ggcgcacaac cttccgtacc gtggcgtgaa
    26401 gagatcgacg catttttaga caggcagagg tgacgcttgg ctacaatgaa agatgttgcc
    26461 cgcctggcgg gcgtttctac ctcaacagtt tctcacgtta tcaataaaga tcgcttcgtc
    26521 agtgaagcga ttaccgccaa agttgaagcg gcgattaaag aactcaatta cgcgccatca
    26581 gccctggcgc gtagcctcaa actcaatcaa acacatacca ttggcatgtt gatcactgcc
    26641 agtaccaatc ctttctattc agaactggtg cgtggcgttg aacgcagctg cttcgaacgc
    26701 ggttatagtc tcgtcctttg caataccgaa ggcgatgaac agcggatgaa tcgcaatctg
    26761 gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac tgtgcaccga aacgcatcaa
    26821 ccttcgcgtg aaatcatgca acgttatccg acagtgccta ctgtgatgat ggactgggct
    26881 ccgttcgatg gcgacagcga tcttattcag gataactcgt tgctgggcgg agacttagca
    26941 acgcaatatc tgatcgataa aggtcatacc cgtatcgcct gtattaccgg tccgctggat
    27001 aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg cgatgaaacg tgcgggtctc
    27061 aacattcctg atggctatga agtcactggt gattttgaat ttaacggcgg gtttgacgcc
    27121 atgcgccaac tgttatcaca tccgctgcgt cctcaggccg tctttaccgg aaatgacgct
    27181 atggctgttg gcgtttacca ggcgttatat caggcagagt tacaggttcc gcaggatatc
    27241 gcggtgattg gctatgacga tatcgaactg gcaagcttta tgacgccacc attaaccact
    27301 atccaccaac cgaaagatga actgggggag ctggcgattg atgtactcat ccatcggata
    27361 acccagccga cccttcagca acaacgatta caacttactc cgattctgat ggaacgcggt
    27421 tcggcttaga tttacgctgt cttttgatca aattattacc atcggttgtt ttcagaagca
    27481 tgaacattgc tgctgaagca acagtaataa tgcccatcgt gataaacgta tagtggaatt
    27541 gttcgacagt cgttgtgcct tccatccctt cataaacgcg aaggacggcc gcacttacag
    27601 caacgcctaa actaatcgac agttgctgcg tgaccgccag aacactgtta ccgctgctgg
    27661 cgttgtcatc ggtcagatcg gcaagtgtga tggtattcat cgcggtaaat tgcgtcgaca
    27721 tagccatccc taatataaac aacggcaaga tcagcatcca tatcgccatt gccggtgatt
    27781 gcaaagagaa ctgagcgatc attagcccaa taatcaccgt gatccccact aacgtatggc
    27841 gatagcccag acgacgtaag acttgggtaa ccatcgattt tgcaataatg gaacctaacg
    27901 ctgtcggtgc catcatacag ccggcaataa acgcctgata accaaatcct acctgtaaca
    27961 tcaatggcat aaggaacggt acacaaccgg tccccagacg ggttgcaata ttgcctacga
    28021 taccgattga gaaagtgcgg gttttaaata aatctaatga aattaatggg tttggcgtgt
    28081 gtcgcgcatg gagaatatag agaagcagta acccgatgct ggtgacaatt accgtcaagg
    28141 caatccagct ggcgacaatc ttttccccga atagctctat tccgcttgag aagagaacaa
    28201 ggctgaggcc aaacagcaaa aagccagtga tatcgaatct gcgtcgtgcg gtggtgaaat
    28261 tgggcatatg tttgcgcgcg taaagaaggc ccgcaatacc tatggggata ttgattaaaa
    28321 atatccagtg ccaggttgcc caggtgacca gcacgccgcc aagaacgggg cctaaaattg
    28381 gccccaccag acccggcatg gcgacaaaat tcaatactgg aagaagttca ttacgaggat
    28441 aagcgcgcag taaggccagc cgagcaacag gcatcatcat tgcgccgcct atcccctgaa
    28501 taacccggaa gacaaccagc tgtggtagcg aattagaaag tgcgcaggcc agagaaccca
    28561 atgtgaacag actcacggca agggtaaaaa tgcgacgcgt accgaagcga tcggctagcc
    28621 atccgcttac cggaataagc atcgccaccg tcagcgtata actgatgatg gctgattgca
    28681 tcgcgagagg agaacgatta aggctatgag cgattgcggg taaggcggta ttcagaatag
    28741 tggcatcaag tgcctgcatg aagaaggcca tcgccgcgat ccgcggcaaa cccgccatac
    28801 tgcgcttctt tttatcgctc attcaatgtc ctgttatcgg gttgtcactt atcaggtgag
    28861 cgtagcagcg cctgacaagc tttaaatgcc gcgtcgccat cgctttggac aatcgcatcg
    28921 acaatcgctt ggtgcagatc cagctttatc actgtgtcgc tggtaattga cgtgaagtaa
    28981 gtgtgataaa ccgaatggaa tagcgaggcg aatgaggtca aaaacggatt ggcgctcatt
    29041 tcatagatat gctcatgcca ggccatatcg acttcgatcc agcgttcacg gcgaaagttc
    29101 tcttttaatg ccgccatttc ggccattaac gtattgagat gcgccttctg ttccgcggtg
    29161 ccaaccgttg cagccagtag gcaagcttgc ggctccagac agatacgcat aaccagaaag
    29221 tgattgatga cctgatgaaa gttctcttct gtcatccacc aggtaagcaa ttcctgatca
    29281 agaaaattcc agtttgattg tggcatgacc cgggtaccaa ttcgcggtcg cggtaaaacc
    29341 atcccttttg ccgttaacgt tttgaccgct tcgcgtaccg ctgtacgact cactccaaat
    29401 tgctcgccca gctcaatctc accaggcaaa atggtgccgg gttcatattc accttttaag
    29461 atgcgttgcg ccagcttctc agccagaaca tacgaaaggt ttttctgtgc agctaactgt
    29521 tgtgcgctta aaggcattac taatcttcct ttttcttttt attcctcctt agtatgccac
    29581 caggaagtgt gattacggtt gcaaaaacgg caaattgctt gttttatggc acattaacgg
    29641 gggttttgct
//
LOCUS       DAZFAH010000038        28639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFAH010000038 DAZFAH010000000
VERSION     DAZFAH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.038"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHJWB_001598"
     CDS             complement(<1..596)
                     /locus_tag="ACHJWB_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4313249.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHJWB_001599"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHJWB_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4313250.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHJWB_001600"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHJWB_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4313251.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHJWB_001601"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHJWB_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4313252.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHJWB_001602"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHJWB_001602"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4313253.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHJWB_001603"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHJWB_001603"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4313254.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHJWB_001604"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHJWB_001604"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4313255.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHJWB_001605"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHJWB_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4313256.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHJWB_001606"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHJWB_001606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4313257.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHJWB_001607"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHJWB_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4313258.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHJWB_001608"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHJWB_001608"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4313259.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHJWB_001609"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHJWB_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4313260.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHJWB_001610"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHJWB_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4313261.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHJWB_001611"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHJWB_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4313262.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHJWB_001612"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHJWB_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4313263.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHJWB_001613"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHJWB_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4313264.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLTQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHJWB_001614"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHJWB_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4313265.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHJWB_001615"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHJWB_001615"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4313266.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHJWB_001616"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHJWB_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4313267.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHJWB_001617"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHJWB_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4313268.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHJWB_001618"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHJWB_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4313269.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHJWB_001619"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHJWB_001619"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4313270.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHJWB_001620"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHJWB_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4313271.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAEKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHJWB_001621"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHJWB_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4313272.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHJWB_001622"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHJWB_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4313273.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHJWB_001623"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHJWB_001623"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4313274.1"
                     /translation="MFKSTLAAMAAVFALSALSTAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHJWB_001624"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHJWB_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4313275.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHJWB_001625"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHJWB_001625"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4313276.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHJWB_001626"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHJWB_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4313277.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26271..27077
                     /gene="nirC"
                     /locus_tag="ACHJWB_001627"
     CDS             26271..27077
                     /gene="nirC"
                     /locus_tag="ACHJWB_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4313278.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27096..28469
                     /gene="cysG"
                     /locus_tag="ACHJWB_001628"
     CDS             27096..28469
                     /gene="cysG"
                     /locus_tag="ACHJWB_001628"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4313279.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6921 a         7706 c         7229 g         6783 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt cttttagaga gttttccatg taacgcccca gcgaggcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaaaca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga ggaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcaacctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcagtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctgg tgtccgcact taacgcattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgacc
     9361 ccgccaacaa cggcactaac gccagtgctg ggatcaccgc caaatcctga aacagcaaca
     9421 ccgagaagcc caactgcccg gattcgctgc gattcatccc tttttcgcgc atcagttgta
     9481 gtgccattgc ggtcgaagac atcgcaagac cgatgccgcc caccaccgcc gcctgccatg
     9541 agaaatgggt tagcatcaat agtcccgcca gtaatgccgc gcttaacagc acctgggctg
     9601 cgccaacgcc aaaaatagag cgccgcaaat gccagagttt ggcagggttt aactccagcc
     9661 cgatgataaa catcaggaac accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa tcgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat acccagccgc gatgccagcg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg actcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacatcgc tcatcgggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtaa tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc gccttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctgc acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt atgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctga ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc aggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag ttcgaaggcg cgctggttgt
    12001 cgtctcgcac gaccgtcatt tgctgcgttc caccactgac gacctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgacgc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg cgtggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga gtttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaagaaggca atgatctccc gattgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcacgcaag ctggcagcct accccggaac actgccgatt
    13141 aatctcacgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatatcatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aagcgaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accctggaat caggtaccgg gaacattcac
    14101 cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tggtgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accacaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaacctgg
    15841 caaaacaacc agatgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ccctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggtaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagct
    16381 ggggccattg cacgcaaaat tgcttcctgc atcgctgctc gccgccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc attgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgcg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagccc
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgaa ctgtggctta agctccgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgcacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgcag
    18841 ccgtcacgcc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcactgcttt cactgcgtgg agatcgttaa agggaacgtg gatgatgtct gccggtttcg
    18961 gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacacagg
    19081 cgtaatgacg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtt gcctcaatca gtttacgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc ataatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcactaaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcac
    19681 gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgcta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 accttttcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgag atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca acgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat caataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgtt ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ttgttgctgg cggatgttca gccggttacg catgatatca aggcctggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctctaca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc cacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcggtaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcgacggcg
    22141 atgttcattc tcggggtggt cagcggcatt actatgtcga ttggtacatt cctgataaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttactgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcactggg caaacatgca ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc taatgccgga cggcactatc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt
    23581 tatgacaagc tgatcatggc aaccggttcc tacccgtgga ttccaccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgttc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtattgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac caggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggt
    24421 aacctgctgc aactggtgct gaacgctatc gaactgccgg aaaacccgga ttccctgatc
    24481 ctgccagcac actctggtag cggcaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagcc gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggtcactca ggtactgaac gcggaactgg cgaaacaggg gatcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaagatcac tggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagccggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattatctga aagcggtgat cattgacgac aaactggggc tgaacgcaca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtacag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtatattgc aggacatttt
    26221 ttaaactttt tgttttattt ttttgttttt attttttaaa ggataatcaa atgtttacag
    26281 acactattaa taagtgtgcg gctaacgctg cgcgcattgc acgcctgtcg gcaaataacc
    26341 cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta tgtgggtctt gggatcatct
    26401 tgattttcac gctcggtaat ttgctcgacc cgtccgtacg tccgctggtg atgggcgcga
    26461 cctttggtat cgccttaacg ctggtgatta tcgccggttc tgaactgttc accggacaca
    26521 ccatgttcct cacctttggg gtaaaagcgg gcagcatcag ccacgggcaa atgtgggcaa
    26581 tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc atgctctata
    26641 gctggggcgg cggtagcctg ctgccggtag ataccagcat cgttcactcc gtcgcgctgg
    26701 ctaaaaccac tgcaccggca atggtactct tcttcaaagg tgcattgtgt aactggctgg
    26761 tttgcctggc aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt atcgctatct
    26821 ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctctatcgct aacatgacgc
    26881 tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta cacgctggcg ggtattggtc
    26941 ataacctgct gtgggtgacg ctgggtaata ctttatcagg tgccgtattc atgggattgg
    27001 gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt aatcaaactg
    27061 aaacggctgc cggttaatta ctaaggggtt tttacgtgga tcatttgcct atattttgcc
    27121 aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa cgcaaagcaa
    27181 ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt attccacagt
    27241 tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt gatgaaagcc
    27301 ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacgcgctt aaccagcgcg
    27361 tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt ggtcgatgcg ccgaaagccg
    27421 ccagctttat tatgccgtcg attattgacc gctcaccgct catggtagcg gtctcctctg
    27481 gcggcacctc tccggttctg gcacgcctgt tgcgcgaaaa acttgaatca ctgctgccgt
    27541 tgcatctggg ccaggtagcg aaatacgccg ggcaattacg cgggcgagtg aaacaacagt
    27601 tcgccacgat gggtgagcgt cgccgtttct gggagaaatt gttcgttaac gatcgcctgg
    27661 cgcagtcgct ggcaaacaac gatcagaaag ccattactga aaccaccgaa cagttaatca
    27721 acgaaccgct tgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg ggcgatgccg
    27781 ggctgctgac gctgaaagga ctgcaacaaa ttcagcaggc agatgtggtg gtctacgacc
    27841 gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgctgatcgc gttttcgtcg
    27901 gcaaacgcgc gggataccac tgtgtaccgc aggaagagat taaccagatc ctgctgcggg
    27961 aagcgcaaaa aggcaaacgc gtggtgcggc tgaaaggtgg cgatccgttt atttttggcc
    28021 gtggtggcga agagctggaa acactgtgca acgcgggtat tccgttctcg gtggttccgg
    28081 gtattaccgc agcttctggt tgctctgcct attcgggtat tccactcacg catcgcgatt
    28141 atgcccagag cgtacgctta attaccggac acttaaaaac cggtggcgag ctggactggg
    28201 aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggttgaat caggccgcga
    28261 ctattcagca aaagctgatt gaacacggta tgcctggcga aatgccggtg gcaattgtcg
    28321 aaaacggaac ggcagtcacg cagcgcgtga ttgacggtac gctcacgcaa ctcggtgaac
    28381 ttgctcagca aatgaacagt ccatcgctaa ttattattgg tcgggttgtt ggcctgcgcg
    28441 ataaactgaa ctggttctcc aaccattaat ttaacccggg ccagagaatt ctggccttct
    28501 taatgactct ttttatatat tcagcaaata aaacatagcc ccttaataaa tattatctgc
    28561 taatgggtta tattcaccgt tacagcgact tacaaaatta agtcattcgc tcactgacgc
    28621 caattaaata ttcctgtat
//
LOCUS       DAZFAH010000039        28506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFAH010000039 DAZFAH010000000
VERSION     DAZFAH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.039"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..713
                     /gene="acrS"
                     /locus_tag="ACHJWB_001629"
     CDS             51..713
                     /gene="acrS"
                     /locus_tag="ACHJWB_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4313280.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(716..895)
                     /gene="yhdU"
                     /locus_tag="ACHJWB_001630"
     CDS             complement(716..895)
                     /gene="yhdU"
                     /locus_tag="ACHJWB_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4313281.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(979..1863)
                     /gene="yhdJ"
                     /locus_tag="ACHJWB_001631"
     CDS             complement(979..1863)
                     /gene="yhdJ"
                     /locus_tag="ACHJWB_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4313282.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            complement(1949..2245)
                     /gene="fis"
                     /locus_tag="ACHJWB_001632"
     CDS             complement(1949..2245)
                     /gene="fis"
                     /locus_tag="ACHJWB_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4313283.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2271..3236)
                     /gene="dusB"
                     /locus_tag="ACHJWB_001633"
     CDS             complement(2271..3236)
                     /gene="dusB"
                     /locus_tag="ACHJWB_001633"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4313284.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSHLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3565..4446)
                     /gene="prmA"
                     /locus_tag="ACHJWB_001634"
     CDS             complement(3565..4446)
                     /gene="prmA"
                     /locus_tag="ACHJWB_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4313285.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4458..5909)
                     /gene="panF"
                     /locus_tag="ACHJWB_001635"
     CDS             complement(4458..5909)
                     /gene="panF"
                     /locus_tag="ACHJWB_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4313286.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETHLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5899..6141)
                     /gene="yhdT"
                     /locus_tag="ACHJWB_001636"
     CDS             complement(5899..6141)
                     /gene="yhdT"
                     /locus_tag="ACHJWB_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4313287.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6250..7599)
                     /gene="accC"
                     /locus_tag="ACHJWB_001637"
     CDS             complement(6250..7599)
                     /gene="accC"
                     /locus_tag="ACHJWB_001637"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4313288.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7610..8080)
                     /gene="accB"
                     /locus_tag="ACHJWB_001638"
     CDS             complement(7610..8080)
                     /gene="accB"
                     /locus_tag="ACHJWB_001638"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4313289.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8946..9776)
                     /locus_tag="ACHJWB_001639"
     CDS             complement(8946..9776)
                     /locus_tag="ACHJWB_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313290.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AITNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(9813..10787)
                     /gene="yhdH"
                     /locus_tag="ACHJWB_001640"
     CDS             complement(9813..10787)
                     /gene="yhdH"
                     /locus_tag="ACHJWB_001640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4313291.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10939..12879
                     /gene="csrD"
                     /locus_tag="ACHJWB_001641"
     CDS             10939..12879
                     /gene="csrD"
                     /locus_tag="ACHJWB_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4313292.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWCSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13184..14227
                     /gene="mreB"
                     /locus_tag="ACHJWB_001642"
     CDS             13184..14227
                     /gene="mreB"
                     /locus_tag="ACHJWB_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4313293.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14293..15396
                     /gene="mreC"
                     /locus_tag="ACHJWB_001643"
     CDS             14293..15396
                     /gene="mreC"
                     /locus_tag="ACHJWB_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4313294.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15396..15884
                     /gene="mreD"
                     /locus_tag="ACHJWB_001644"
     CDS             15396..15884
                     /gene="mreD"
                     /locus_tag="ACHJWB_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4313295.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15893..16486
                     /gene="yhdE"
                     /locus_tag="ACHJWB_001645"
     CDS             15893..16486
                     /gene="yhdE"
                     /locus_tag="ACHJWB_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4313296.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVATDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16476..17945
                     /gene="rng"
                     /locus_tag="ACHJWB_001646"
     CDS             16476..17945
                     /gene="rng"
                     /locus_tag="ACHJWB_001646"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4313297.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18013..21813
                     /gene="yhdP"
                     /locus_tag="ACHJWB_001647"
     CDS             18013..21813
                     /gene="yhdP"
                     /locus_tag="ACHJWB_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4313298.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDRLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGVTLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21969..23414
                     /gene="tldD"
                     /locus_tag="ACHJWB_001648"
     CDS             21969..23414
                     /gene="tldD"
                     /locus_tag="ACHJWB_001648"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4313299.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23542..24471)
                     /gene="aaeR"
                     /locus_tag="ACHJWB_001649"
     CDS             complement(23542..24471)
                     /gene="aaeR"
                     /locus_tag="ACHJWB_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4313300.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24654..24857
                     /gene="aaeX"
                     /locus_tag="ACHJWB_001650"
     CDS             24654..24857
                     /gene="aaeX"
                     /locus_tag="ACHJWB_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4313301.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24865..25797
                     /gene="aaeA"
                     /locus_tag="ACHJWB_001651"
     CDS             24865..25797
                     /gene="aaeA"
                     /locus_tag="ACHJWB_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4313302.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25803..27770
                     /gene="aaeB"
                     /locus_tag="ACHJWB_001652"
     CDS             25803..27770
                     /gene="aaeB"
                     /locus_tag="ACHJWB_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4313303.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFNT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27862..28134
                     /gene="yhcO"
                     /locus_tag="ACHJWB_001653"
     CDS             27862..28134
                     /gene="yhcO"
                     /locus_tag="ACHJWB_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4313304.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28189..28452)
                     /gene="yhcN"
                     /locus_tag="ACHJWB_001654"
     CDS             complement(28189..28452)
                     /gene="yhcN"
                     /locus_tag="ACHJWB_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4313305.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6818 a         7131 c         7579 g         6978 t
ORIGIN      
        1 gctattttct cactctgtgt cgaatatatt tatttcctga ataattaatc atggcaaaaa
       61 gaaccaaagc cgaagctctg aagacccggc aagaactgat tgaaactgcc atcgcccagt
      121 ttgcgcagca tggcgtaagc aagacgacac tcaacgacat tgccgacgct gctaacgtta
      181 cgcgtggcgc tatctactgg cacttcgaaa acaagactca actgtttaat gagatgtggt
      241 tgcaacagcc ttcattgcga gagttaatcc aggatcactt gacggctgga ttagagcatg
      301 acccgtttca acaattgcgt gaaaaattga ttgtcggctt gcaatatatt gccaaaattc
      361 cccgccagca ggcgttgctg aaaatcttat atcacaaatg tgaatttaat gatgagatgc
      421 tggccgaggg agtgatacgc gaaaagatgg gcttcaatcc gcagactctc cgcgaagtat
      481 tgcaggcgtg tcagcaacaa ggttgtgtag caaataacct cgatttagat gttgtgatga
      541 ttattattaa tggtgccttc agcggaattg ttcaaaactg gttaatgaat atggcgggtt
      601 atgatcttta taaacaagcc cccgctctgg tcgataacgt attaagaatg ttcatgccag
      661 atgaaaacat aacgaaatta attcatcaaa cgaatgaatt aagtgtcatg taaattcaca
      721 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
      781 tattgcggca tttgtctgtt tctgttgcca gcagttcaat ccactgcatc agtaaagtat
      841 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      901 gagcctgtgt atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      961 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc taacccgaca
     1021 tcgttttgac cggttgcccg tctttctttt tttcactttc gccagttcct ccgcggagta
     1081 atgtgacgcg acatccagtc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
     1141 accaatgaat tttcgcccgc tggcgacggc tacggcaccg gtagtaaagc taccggcaaa
     1201 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
     1261 ttccggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
     1321 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
     1381 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
     1441 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
     1501 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
     1561 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
     1621 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
     1681 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1741 tgggtcggca aagatcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1801 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1861 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1921 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1981 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     2041 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     2101 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     2161 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     2221 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     2281 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     2341 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     2401 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     2461 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     2521 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     2581 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     2641 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     2701 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2761 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2821 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2881 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agctgggcaa
     2941 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     3001 atttctttcg gatcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     3061 accatccgta aatgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     3121 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     3181 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     3241 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     3301 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     3361 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     3421 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     3481 tttgcgtaaa taaccggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     3541 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     3601 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     3661 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3721 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3781 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3841 ttcggcgttg tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3901 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3961 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     4021 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     4081 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     4141 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     4201 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     4261 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     4321 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     4381 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     4441 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     4501 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     4561 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     4621 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     4681 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4741 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4801 gacatccgct tcagatgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4861 tctttaatga tcgtagcgga actttgcagc aactgggcat tgatcgtcga catgatcgca
     4921 gccataggtg cagccaggaa gattccggca gcaaacggtg gcagcacttt taccattaac
     4981 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     5041 gccaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     5101 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     5161 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     5221 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     5281 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     5341 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     5401 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     5461 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     5521 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     5581 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     5641 gaaagccaga ctgcaggaag ctgaatcatc gccagcaata cccagcccag gccgtattta
     5701 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5761 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5821 ccggtgctcc gtttacgcat cgcataaaca gagataccga acaccaccac cagataggcg
     5881 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5941 aaatttcacc atcgcccagc acagtccaat aaacagcagc ggcgtcagga tgcaggccat
     6001 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcggctac
     6061 taaccatacc gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     6121 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     6181 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     6241 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     6301 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     6361 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     6421 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     6481 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     6541 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     6601 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     6661 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6721 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6781 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6841 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6901 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6961 ctgaatctcg acgtggcgag gattttccag gtatttctcc atgtaaacca tatcgttgct
     7021 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     7081 gcgcactacg cgcataccgc gaccaccgcc gccgccggag gctttgataa tcaccggata
     7141 accaatgcgt ttagcaatgg cacggttttt atccatatcg tcgcccaacg ggccgtcaga
     7201 acccggtacg caagggacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     7261 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     7321 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     7381 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     7441 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     7501 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     7561 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     7621 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     7681 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7741 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7801 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7861 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7921 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7981 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     8041 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     8101 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     8161 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc caagagtgtc
     8221 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     8281 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     8341 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     8401 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     8461 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     8521 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     8581 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     8641 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     8701 ccgactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaaaaaaat
     8761 cagattattc accccatttt gcagaaggta aactgttttg tcaggcggca gtgcggctat
     8821 ctgatgcttt cagaatttgc cacctgacaa taaaaaagcc gatttggtag caaaatcggc
     8881 ttcggtgagt ttgcagcatt catcacatac tccgacaagc cttttcaagc atgacggagg
     8941 ttgcgttaat gcaaggtttc tacccaggga taaaagtgac cgttgcggcg gatgaatatc
     9001 tgtgttgtca gaggatggtc atcctcacgg aagcattgtt ctgttagctg tcgtaatgcg
     9061 ctgagatttg ctggctgatc attgtgggtg aaaaacagtt ttcctctgga gggaacggtc
     9121 actaacaggt cgccgcattc aggatcgggt gattgcaccc aaagatcgtc aatgagcatc
     9181 agactgggct cataagtacc ccctgcggag ataacggcaa cttttccatc gtttgcgcct
     9241 gtatcaacga tttcagccgt aacttccggc aatatttgct tcaaattagt gatggcttgt
     9301 tcgcggtcaa tggtcacttc gtagcgcatc agatagcgca tggtttcgct accatcttca
     9361 gcaaacaaga taattaactc atcgttaaag tcttcccagg caaaatccag cgaggcgttg
     9421 aggacctctg gatcagactc atactggcta agctgttggc gaacggtatc cagccaggct
     9481 ttttctttaa tcaccggaac aatacagcgc ccggtcggca gcatttctgc aagctgtttc
     9541 agcatatctg cagccacttc ttgcagcggg tctggtgcgg cttgataacg tttgtaggca
     9601 tcatcaaggg tgatttccac cgtttggtga cccgtatcaa tgcgcatcaa tttgtggtca
     9661 gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcctgacc aaggtgttcg
     9721 acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga tgtcaaaatg
     9781 agttccctct cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc
     9841 tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct
     9901 tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt
     9961 gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga
    10021 ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg
    10081 cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca
    10141 attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg
    10201 agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg
    10261 gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca
    10321 ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc
    10381 atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc
    10441 agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgttc cgccagccca
    10501 ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa
    10561 cgcggatctt cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa
    10621 ttacggatga tttttccctt accggtaatc gccagcgcat ctttgtagtt caggctcgac
    10681 cagtgaacat caaccgtgac atcgccctcc ggcaggcgac tttcgtccag agtctgtacc
    10741 gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat
    10801 gtgcatgatg gattggaaaa tcatttctga agactatact cgctaatgga aaagcaaagc
    10861 cgatgaagcg caataaattg cgagataatt ctgatttgct agtatgcccg cttcctcact
    10921 atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc
    10981 accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta caacgccatt
    11041 cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccacctagtg
    11101 tcgaatgact tcagcacatt aaggccacaa attaccgaat taatgatgtc ggcagatatc
    11161 gttcgtgtag acctgctgca tggtgataaa caggtttata ccctggccag aaatggtagt
    11221 tatcgtccgg ttggcaccag cgatctgttt cgtgaactga gcgttccgtt gataaagcat
    11281 ccggggatgt cgctgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg
    11341 atgaccaccg cgccgctcac gggggcgatt agctttatca ttgttatgct cttcctggcg
    11401 gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt
    11461 atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga
    11521 accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc
    11581 cgtcttgata cgctgatccg ctcttatgcc gcccaggacg tgaaaaccgg cctcaataac
    11641 cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc
    11701 cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacttggggg
    11761 cacagccagg ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac atttatgatg
    11821 cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg
    11881 caccggacgt taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc
    11941 ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg
    12001 tgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg
    12061 ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc
    12121 ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt
    12181 tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc
    12241 atcttcgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt
    12301 ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac
    12361 tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt
    12421 cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt
    12481 attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt
    12541 ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc
    12601 accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga
    12661 aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctcc
    12721 gggaccagca cccaagttta cgccaccggt gtgcgttcgc gaagcgagtg gcagaccctg
    12781 attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat
    12841 actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta atctgttttc
    12901 acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg
    12961 cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat
    13021 gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat
    13081 tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag
    13141 cggattttct tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg
    13201 tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt
    13261 aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc
    13321 cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac
    13381 gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt
    13441 gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc
    13501 aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat
    13561 tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc
    13621 tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat
    13681 cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc
    13741 cgtgcgcatt ggtggtgatc gtttcgacga agctatcatc aactatgtgc gtcgtaatta
    13801 cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta
    13861 cccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc
    13921 acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg
    13981 tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat
    14041 ctccgagcgc ggcatggtgc tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt
    14101 gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc
    14161 gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga
    14221 agagtaatcg gatgcaggca ggggaaatgt ctgtttaccc tgcctggtct gatacgagaa
    14281 tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt
    14341 ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt
    14401 caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct
    14461 cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa
    14521 aaccgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa
    14581 tacaaacagg agaatgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag
    14641 cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt
    14701 atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt
    14761 gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat
    14821 gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt
    14881 aacggttgta cggatgattt gcagcttgag catctaccag cgaatacgga tattcgtgtt
    14941 ggtgatgtgc tggtgacttc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg
    15001 gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg
    15061 acagcagggc tgcaacgttt gcgttatctg ctgctgctgt ggggggcaga tcgtaacggc
    15121 gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg
    15181 atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg
    15241 gggatcgctc agccgactcc gcagcaaccg acgacaggaa atgcagctac tgcgcctgct
    15301 gcgccgacac agcctgctgc taaccgctct ccacaaaggg ctacgccgcc gcaaagtggt
    15361 gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac
    15421 gttgggtaat ctggctctct ttcctcattg cgctgttgct gcaaatcatg ccctggccgg
    15481 ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat tggatcctgg
    15541 ccttgcctca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga
    15601 tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg
    15661 cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt
    15721 tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct
    15781 ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt
    15841 tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc
    15901 tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggcgtgac
    15961 ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca
    16021 gtatgttgtg cgtctggcgc gcgagaaagc acaggcgggt gtcgcgcaaa cggcgcagga
    16081 tctcccggtg ctgggtgcgg atactatcgt tatcctgaat ggagaagtgc tggagaaacc
    16141 gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt
    16201 gatgacggca gtggcgttgg ccgacagcca gcacattctc gattgcctgg tggccaccga
    16261 tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc
    16321 gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat
    16381 aaatggcagc tatcacgccg tagtcggctt accgctggtt gaaacgtatg aattattaag
    16441 taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg
    16501 taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata
    16561 ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg
    16621 tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc
    16681 atgcatccga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa aagcaattca
    16741 cggtgcgcga catttcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga
    16801 aagatccgct tggcactaag ggggcgcgcc tgaccaccga tatcacgctg ccttctcgct
    16861 atctggtctt tatgccaggg gcttctcacg ttggtgtttc ccaacgtatt gaaagcgaat
    16921 cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta
    16981 tcatccgtac cgcagcggaa ggggttggcg aggctgaact ggcctccgat gccgcttatc
    17041 tgaaacgcgt ctggaccaaa gttatggagc gtaaaaaacg cccgcagacc cgttatcagc
    17101 tgtacggcga actggcgctg gcgcagcgtg ttctgcgtga tttcgccgac gccgaactgg
    17161 accgcattcg cgttgactca cgcctgactt acgaagcatt gctggagttt acctcggagt
    17221 acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg attttcgatc
    17281 tctttgatgt cgaaaacgaa atccagcgag cgctggaacg caaagtagaa ctgaaatccg
    17341 gtggttatct cattatcgac cagaccgaag cgatgaccac cgtggacatc aataccggag
    17401 cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa taccaatatt gaagcgacgc
    17461 aggctatcgc tcgccagtta cggttgcgta atctgggtgg gattatcatt attgatttca
    17521 tcgatatgaa taatgaagat caccgccgcc gcgtgctgca ctcgctggag caggcgttga
    17581 gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga
    17641 cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc
    17701 acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg
    17761 ttcgtgtcca ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag
    17821 ctgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggca
    17881 aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga
    17941 tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt tttagcagac
    18001 aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt
    18061 gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct tgacgcctgg
    18121 cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag
    18181 ctctcagcca gctggcagaa ttttggcccg acgcttgaag cacacgacat ccgtgcagaa
    18241 ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag
    18301 agcttgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct gcgctttcgc
    18361 accaacactc ctatcaccag cggtggtggt aatgatagac tggaagccag tcacatcagc
    18421 gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagttttctg
    18481 acgccatccg gtcagcgcgc cgagctggcg atcccacaac tcacctggct gaacgatcca
    18541 cgtcgacacc gtgcggaagg cctggtaagc ctctctagcc ttaccggaca gcacggcgtg
    18601 atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg
    18661 ctacaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt
    18721 gctctggaaa cggcacagtt ctcccttgaa ggctggatga cgatcgacaa aggcgatgta
    18781 accggcggtg acgtctggct gaaacagggc ggtgccagct ggttgggcga gaagcaaacg
    18841 catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggctggcag
    18901 ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg
    18961 acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa
    19021 ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc
    19081 gcgaaacttt cacctgcact gggtgatgtt tggcgctcca cacaaccgag cggcaagatt
    19141 aacactctgg cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg
    19201 tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg
    19261 acgctttccg gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg
    19321 ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc
    19381 tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa
    19441 gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg
    19501 ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta tttcccggaa
    19561 aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg cggtgaagcg
    19621 gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa
    19681 ggtcagtttg aagtgctggt gccgctgcgt aacgcgaagt ttgccttcca gccggactgg
    19741 cctgcattaa ctaaccttga tattgaactg gactttatta acgacggttt atggatgaaa
    19801 accgatgacg ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac
    19861 tactcgaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc
    19921 ccttactttg atgagacacc gctgaaagat tctctgggtg tgaccctgca agaactccag
    19981 ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgaactggta
    20041 accgcgaaag gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc
    20101 accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca
    20161 ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca
    20221 aaagcctacc aggttgcggt aaacctcaac ggtaactggc aaccggcgaa aaccggcgtt
    20281 ctgcctgaag cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc
    20341 attgagctgc cttatcatgc tggcgcgacc tataacgtag agctgaacgg cgatctgaag
    20401 aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actagcggta
    20461 aacgttaagg ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat
    20521 aaccatttca atagccgctg gttgcttggt caaaagctga cgctcgatcg tgctatttgg
    20581 gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga actcaatatg
    20641 ccgccgatga atggtgccga gtggctggcc ctgttccaga aaggcgctgc ggagagtgtc
    20701 ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt gtcactggga
    20761 aatcagcaat ggaataacct gagtattgtt tcgcaaccga cggcaaatgg cacccaggtt
    20821 gaggcgcaag ggcgtgaaat caacgccacg ctagcgatgc gtaataacgc gccgtggctg
    20881 gcgaatatca aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaacg
    20941 ccgtcatcac ctttcccgac aacggagcgc attaacttcc gtggctggcc ggacgcacaa
    21001 atacgatgca cagagtgctg gttctggggg caaaaattcg gtcgcattga cagtgatctc
    21061 accatttctg gcgatacgtt aacgctgacc aatggactga ttgatactgg tttctcgcgg
    21121 cttactgccg atggtgaatg ggttaataat ccggggaatg aacgtacctc gctgaaagga
    21181 aaactgcgcg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata
    21241 cgccagtcgt catttaatgt ggattacgat ttacactggc gcaaagcacc ctggcagcca
    21301 gatgaagcga cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa
    21361 atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt
    21421 aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc
    21481 agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt ggatggcctg
    21541 gaggcggata tcgccatgaa agggtcggta aatctggtac gtcgcgacct gaatatggaa
    21601 gcggttgtcg caccagagat ttctgcgacg gtgggcgtgg ctgcggcttt tgcggttaac
    21661 cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct gtggagcaaa
    21721 gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg
    21781 ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa
    21841 tctcatatga taatcgttgc caaaggccaa cgagccggaa cataactgta ggccggatag
    21901 ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag
    21961 caaaaacgat gagtctgaac ctggtaagtg aacaattgct agcggcgaac ggcctgaaac
    22021 atcaggactt gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc
    22081 tctattttca gtcgagctat cacgaatcct gggttttaga agaccgcatt attaaagatg
    22141 gttcttacaa catcgatcag ggcgttggtg tgcgtgcaat cagtggtgaa aaaaccggat
    22201 ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca
    22261 ccatcgtccg tgatagtggt gatggcaaag tacagacgct gggcgcggta gagcatagcc
    22321 cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc
    22381 tgcgtcgcgt cgataaggtt gctcgcgaag cggacaagcg cgtacaggaa gtgactgcca
    22441 gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg
    22501 atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg
    22561 aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatcttg
    22621 acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc
    22681 tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg
    22741 gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgcggca
    22801 cttcagtatt tagtgggcag gtcggggagc tggtggcttc agaactgtgt accgtggttg
    22861 atgacggcac gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag
    22921 gccagtacaa cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca
    22981 acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc
    23041 tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc ccgcaggaaa
    23101 ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata
    23161 tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag
    23221 taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga
    23281 tttcgatggt tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag
    23341 ggcaaagttt gccggttggc gtgggccagc caacgctgaa agttgataac ctgactgttg
    23401 gcggtactgc gtaataattt cattattttc agaaggataa ttaatctttc tacatgcacg
    23461 aacggtcccc tcgctcctct ggggttaggg tgaggggaac ccgttggcac aggtttgtag
    23521 aacgtaacag tacaatatga attacttctc tttcccgcgc ccgtgcatct cctgaaacaa
    23581 tttaccgacc tcaacaaaat aatccgtcag cgagttgatc acgacctgta ccttcagcgg
    23641 cagcttatct ttttcggtat ataacgcata aaccgggcgt ggatctgact gataacgcgg
    23701 cagcaggatc tccagctcac cgcgattgat ctcgttgatc acccacatca gcggcacgta
    23761 ggcgatcccg gcacccgccg ttagccagcg caccagcgtc atcggatcat tagtcacaaa
    23821 tctcccttgc gggatcaggc gagtcgagat cccttccggt gcaatcagtt caaattcatt
    23881 gtcgggccgc acgctgtatt caagccatga atggctactt aaatcggcgg gtttttccgg
    23941 tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc accattggca tcgcgccaag
    24001 acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg cggatcacca catccagacc
    24061 gtcggcaatc aggtcggggg ctggaattcc ggtaaccaga ttgacgctca aacctgggta
    24121 ttctttcagc atttttgctg tcagcccggc gagaacattt tgtgccatag ttgaagaaca
    24181 gccaatgcgc agcgtcccga tgggggtgtt attgaaggca tacagttgct catgaacatc
    24241 ctgcacttca tgaagcatcc gacggcagcc ctggtagtaa attctgccgg cttcggtcag
    24301 gccaatgctg cgtgtgctac ggtttaacag ctttacctgc aactcatctt ccagttttga
    24361 caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga
    24421 gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca ttattcactc
    24481 tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt acgctaatat
    24541 attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg
    24601 cgacagatgc tgaaaataat aacgcctgct ctctctttac aaccaaggtc aacatgagtc
    24661 tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt
    24721 tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact
    24781 ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat
    24841 cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc
    24901 atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac
    24961 accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg
    25021 gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga
    25081 cagatactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc
    25141 gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac
    25201 cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggctaacaa cgtactacaa
    25261 acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt
    25321 gaacgcacgg tgatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc
    25381 ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa ctccttctat
    25441 gtactggcct atatggaaga aactaagctg gaaggggtgc gtccggggta tcgtgcagag
    25501 atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgcaggg
    25561 gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgactataga ctctaacctt
    25621 gaatgggtgc gtcttgcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag
    25681 aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac
    25741 gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca
    25801 cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg
    25861 ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg
    25921 tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt
    25981 ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg
    26041 ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct
    26101 gtatctgggc tggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtacg
    26161 cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat
    26221 tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg
    26281 cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc
    26341 tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg
    26401 gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg caccacggcg ctacaaggca
    26461 tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag
    26521 cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga
    26581 atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttaacacg ccggtagaaa
    26641 ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc tggaccgggg
    26701 aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc
    26761 tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag
    26821 gcgaaccgga agtcaaagta gagtcagccg aacgtcatca tgcgatggtt aacttctggc
    26881 gaaccacact ttcctgcatt ctgggcacgc ttttctggct gtggacgggc tggacttccg
    26941 gcagtggtgc aatggtgatg attgcggtag tgacgtcact ggcaatgcgt ttgccgaatc
    27001 cacgcatggt ggcgatcgac tttatctacg ggacgctggc cgcgctgccg ttagggctgc
    27061 tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc
    27121 tggcagtgct gggatttttc ctcggtatag aagtacagaa acggcgactg ggctcgatgg
    27181 gggcgctggc cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca
    27241 gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta
    27301 ttttgcttgt gcgggataaa tcgcgcgaca ggaccggacg tgtactgctt aatcagtttg
    27361 tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc
    27421 cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat
    27481 ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg
    27541 ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat
    27601 ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaactggaaa
    27661 tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc
    27721 ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg
    27781 acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc
    27841 gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt
    27901 caggaggatt tttatcgtga ctttagccaa acctttggtc tggctaaaga taaggtacgc
    27961 gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt
    28021 gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt
    28081 gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa
    28141 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    28201 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    28261 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    28321 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
    28381 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    28441 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc
    28501 catatc
//
LOCUS       DAZFAH010000040        28018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFAH010000040 DAZFAH010000000
VERSION     DAZFAH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.040"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..813
                     /locus_tag="ACHJWB_001655"
     CDS             <1..813
                     /locus_tag="ACHJWB_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4313306.1"
                     /translation="VLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLK
                     RIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMA
                     NHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAI
                     SCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNAR
                     DSKHAGRMFSMHNCQINPGAGPEITTPWRSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1003..1116
                     /gene="yhiY"
                     /locus_tag="ACHJWB_001656"
     CDS             1003..1116
                     /gene="yhiY"
                     /locus_tag="ACHJWB_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4313307.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1179..2246
                     /gene="yhiI"
                     /locus_tag="ACHJWB_001657"
     CDS             1179..2246
                     /gene="yhiI"
                     /locus_tag="ACHJWB_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4313308.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2243..4978
                     /gene="rbbA"
                     /locus_tag="ACHJWB_001658"
     CDS             2243..4978
                     /gene="rbbA"
                     /locus_tag="ACHJWB_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4313309.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4978..6102
                     /gene="yhhJ"
                     /locus_tag="ACHJWB_001659"
     CDS             4978..6102
                     /gene="yhhJ"
                     /locus_tag="ACHJWB_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4313310.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6227..6502
                     /locus_tag="ACHJWB_001660"
     CDS             6227..6502
                     /locus_tag="ACHJWB_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4313311.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6499..6858
                     /locus_tag="ACHJWB_001661"
     CDS             6499..6858
                     /locus_tag="ACHJWB_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4313312.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLSLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6978..7379)
                     /gene="nikR"
                     /locus_tag="ACHJWB_001662"
     CDS             complement(6978..7379)
                     /gene="nikR"
                     /locus_tag="ACHJWB_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4313313.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7385..8191)
                     /gene="nikE"
                     /locus_tag="ACHJWB_001663"
     CDS             complement(7385..8191)
                     /gene="nikE"
                     /locus_tag="ACHJWB_001663"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4313314.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVTLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLNAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8188..8952)
                     /gene="nikD"
                     /locus_tag="ACHJWB_001664"
     CDS             complement(8188..8952)
                     /gene="nikD"
                     /locus_tag="ACHJWB_001664"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4313315.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(8952..9785)
                     /gene="nikC"
                     /locus_tag="ACHJWB_001665"
     CDS             complement(8952..9785)
                     /gene="nikC"
                     /locus_tag="ACHJWB_001665"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4313316.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYAPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9782..10726)
                     /gene="nikB"
                     /locus_tag="ACHJWB_001666"
     CDS             complement(9782..10726)
                     /gene="nikB"
                     /locus_tag="ACHJWB_001666"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4313317.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10726..12300)
                     /gene="nikA"
                     /locus_tag="ACHJWB_001667"
     CDS             complement(10726..12300)
                     /gene="nikA"
                     /locus_tag="ACHJWB_001667"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4313318.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12411..12998)
                     /gene="acpT"
                     /locus_tag="ACHJWB_001668"
     CDS             complement(12411..12998)
                     /gene="acpT"
                     /locus_tag="ACHJWB_001668"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4313319.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13053..14102)
                     /gene="yhhT"
                     /locus_tag="ACHJWB_001669"
     CDS             complement(13053..14102)
                     /gene="yhhT"
                     /locus_tag="ACHJWB_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4313320.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14234..15451
                     /gene="yhhS"
                     /locus_tag="ACHJWB_001670"
     CDS             14234..15451
                     /gene="yhhS"
                     /locus_tag="ACHJWB_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313321.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15455..16012)
                     /gene="dcrB"
                     /locus_tag="ACHJWB_001671"
     CDS             complement(15455..16012)
                     /gene="dcrB"
                     /locus_tag="ACHJWB_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4313322.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16085..16750)
                     /gene="yhhQ"
                     /locus_tag="ACHJWB_001672"
     CDS             complement(16085..16750)
                     /gene="yhhQ"
                     /locus_tag="ACHJWB_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4313323.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16971..17216
                     /gene="tusA"
                     /locus_tag="ACHJWB_001673"
     CDS             16971..17216
                     /gene="tusA"
                     /locus_tag="ACHJWB_001673"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4313324.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17318..19516)
                     /gene="zntA"
                     /locus_tag="ACHJWB_001674"
     CDS             complement(17318..19516)
                     /gene="zntA"
                     /locus_tag="ACHJWB_001674"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4313325.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTAVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKATAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19590..20216)
                     /gene="yhhN"
                     /locus_tag="ACHJWB_001675"
     CDS             complement(19590..20216)
                     /gene="yhhN"
                     /locus_tag="ACHJWB_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4313326.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20357..20716
                     /gene="yhhM"
                     /locus_tag="ACHJWB_001676"
     CDS             20357..20716
                     /gene="yhhM"
                     /locus_tag="ACHJWB_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4313327.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLEAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20719..20988)
                     /gene="yhhL"
                     /locus_tag="ACHJWB_001677"
     CDS             complement(20719..20988)
                     /gene="yhhL"
                     /locus_tag="ACHJWB_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4313328.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20978..21574)
                     /gene="rsmD"
                     /locus_tag="ACHJWB_001678"
     CDS             complement(20978..21574)
                     /gene="rsmD"
                     /locus_tag="ACHJWB_001678"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4313329.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21724..23220
                     /gene="ftsY"
                     /locus_tag="ACHJWB_001679"
     CDS             21724..23220
                     /gene="ftsY"
                     /locus_tag="ACHJWB_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4313330.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23223..23891
                     /gene="ftsE"
                     /locus_tag="ACHJWB_001680"
     CDS             23223..23891
                     /gene="ftsE"
                     /locus_tag="ACHJWB_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4313331.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23884..24942
                     /gene="ftsX"
                     /locus_tag="ACHJWB_001681"
     CDS             23884..24942
                     /gene="ftsX"
                     /locus_tag="ACHJWB_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4313332.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKLFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25187..26041
                     /gene="rpoH"
                     /locus_tag="ACHJWB_001682"
     CDS             25187..26041
                     /gene="rpoH"
                     /locus_tag="ACHJWB_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4313333.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26312..27415
                     /gene="livJ"
                     /locus_tag="ACHJWB_001683"
     CDS             26312..27415
                     /gene="livJ"
                     /locus_tag="ACHJWB_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4313334.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27614..27997)
                     /gene="panM"
                     /locus_tag="ACHJWB_001684"
     CDS             complement(27614..27997)
                     /gene="panM"
                     /locus_tag="ACHJWB_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4313335.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6654 a         7287 c         7774 g         6303 t
ORIGIN      
        1 gttttagaaa gtcttcagcc ttatttggtt attaataaag acgcagtcac gccatgtacg
       61 tttctgggcg accagactgg cgatcgattt agtaatatct gtggcgataa atttatcatc
      121 gatttattaa aacgcataat gagtatcaat gaaaacgttc atgtattagc tggaaatcat
      181 gaaacgaatt gcaatggtaa ctatatgcag aacttcacgc gcatgaaacc acttgatgag
      241 gatacctact ctgggataaa agattatcct gtatgtttct atgaccccaa atacaaaata
      301 atggcaaatc atcatgggat aacttttgat gatcagcgaa aacgttacat tataggaccg
      361 ataactgtct ctattgatga aatgaccaat gctctcgatc cggtcgaatt agcggaaatt
      421 attaataaaa agcatcatgc tataattaat ggtaaaaagt ttaaaactag ccgggctatc
      481 tcttgtcgat cctttaatcg ttatttttct gtttcgacag attacagacc taaacttgaa
      541 gcactattag catgctcaca aatgttaggt ataaatcaag ttgttgcaca taacggaaat
      601 ggtgggagag aacgcatagg tgaaacgggg acagttttag gtcttaacgc ccgtgacagt
      661 aaacatgctg gaagaatgtt cagcatgcat aattgccaaa ttaaccctgg tgcagggcct
      721 gagataacca ctccctggag gtcttaccag catgaaaaaa acagaaatgg acttatgcct
      781 ttgatcagaa ggcgcacaat gttacagctc tgaaccatag acaacatgaa tattaatgtt
      841 actatctggt tgagactgtt gataaaacgt aaaaaggaat gctctgttcc tgcaactgaa
      901 caggcgtgaa acgcgatcca acaggatgga taccgttttg tcagccagtt ttatagatca
      961 cacttatgag tatgcgtgtt tcatttacgg ccaggaatgc tgatgatgac aacattacta
     1021 cccgttttca ctaagccttc cccgttggcg ctcaatgctc tgcgcgctgg tcgtatttgt
     1081 cgtttccttc ttatcccgga tggaagaatc cgctgaacgt cgttcggcgg cagttgtaac
     1141 acgcgtcgtt tgcttacagg gattatggcg ggtcgattat ggataagagt aagcgccatc
     1201 tggcgtggtg ggttgtcggg gcactggcgg tggctgccgt cgtggcgtgg tggctgttgc
     1261 gcccggcggg cgtgccggaa ggctttgctg tcagcaatgg gcgcattgaa gcgacggaag
     1321 tggatatcgc cagcaaaatt gccgggcgta tcgacaccat tctggtgaaa gaaggccagt
     1381 ttgttcgcga aggcgaagtg ctggcgaaga tggatactcg cgtgttgcag gaacagcgac
     1441 tggaagccat cgcgcaaatc aaagaggcac aaagcgccgt tgccgccgcg caggctttgc
     1501 tggagcaacg acaaagtgaa actcgtgccg cacagtcgct ggtcaatcaa cgccaggccg
     1561 aactggactc cgttgccaaa cgtcatacgc gttcccgctc gctcgcccaa cgaggggcta
     1621 tttctgcgca acagctggat gacgatcgcg ccgccgctga gagcgcccga gcggcgctgg
     1681 aatcggcaaa agctcaggtt tctgcttcta aagccgctat agaggcggca cgcaccaata
     1741 tcattcaggc gcaaacccgc gtcgaagcgg cacaagccac tgaacggcgc attgccgcag
     1801 atatcgatga cagcgaactg aaagccccgc gtgacggacg cgtgcagtat cgggttgccg
     1861 agccagggga agtgctggcg gcaggcggtc gggtgctgaa tatggtcgat ctcagcgacg
     1921 tctatatgac tttcttcctg ccaaccgaac aggcgggcac gctaaaactg ggcggtgaag
     1981 cacggctgat cctcgatgcc gcgccagatc tgcgtattcc ggcgaccatc agttttgtcg
     2041 ccagcgtcgc ccagttcacg ccaaaaaccg tcgaaaccag cgatgaacgg ctgaaactga
     2101 tgttccgcgt caaagcgcgt atcccaccgg aattactcca gcagcatctg gaatatgtca
     2161 aaacgggctt gccgggcgta gcgtgggtgc gggtgaatga agaacttccg tggcctgacg
     2221 acctggtggt gaggttgccg caatgacgca tctggaactg gttcccgtcc cgcctgtcgc
     2281 gcaactggcg ggcgtgagcc agcattatgg aaaaaccgtt gcgctgaaca atatcactct
     2341 cgatattccg gcccgctgta tggtcgggct gattggcccg gacggcgtcg ggaagtcgag
     2401 cttgttgtcg ttgatttccg gtgcccgcgt cattgagcag ggcaatgtga tggtgctggg
     2461 cggcgatatg cgcgacccga agcatcgccg cgacgtctgc ccgcgcattg cctggatgcc
     2521 gcaggggctg ggcaaaaatc tctaccacac cttgtcggtg tatgaaaacg tcgatttttt
     2581 cgcccgcctg ttcggtcacg acaaagcgga gcgggaagtg cgaattaatg agttgctgac
     2641 cagcaccggg ttagcaccgt ttcgcgatcg tccggcaggt aaactctccg gcgggatgaa
     2701 gcaaaaactt gggctgtgct gcgcgttaat ccacgacccg gaactgttga tccttgatga
     2761 gccaacaacg ggggttgacc cgctctcccg cgcccagttc tgggatctga tcgacagtat
     2821 tcgccagcgg cagagcaata tgagcgtgct ggtcgccacc gcctatatgg aagaggccga
     2881 acgcttcgac tggctggtag cgatgaatgc cggagaagtg ctggcaactg gcagcgccga
     2941 agagctgcgg cagcaaacgc aaagcgccac gctggaagag gcgtttataa atctgttacc
     3001 gcaagcgcaa cgccaggcgc atcaagcggt agtgatccca ccgtatcaac ctgaaaatgc
     3061 agagattgcc atcgaagcgc gcgatctgac catgcgtttt ggttccttcg ttgccgttga
     3121 tcacgtcaat ttccgcattc cacgcgggga gatttttggt tttcttggat cgaacggctg
     3181 cggtaaatcc accaccatga aaatgctcac tggactgctg cccgccagcg aaggtgaggc
     3241 gtggctgttt gggcaaccgg ttgatccaaa agatatcgat acccgccgtc gggtgggcta
     3301 tatgtcgcag gcgttttcgc tctataacga actcaccgtg cggcaaaacc ttgagttaca
     3361 tgcccgtttg tttcacatcc cggaagcgga aattcccgca agagtggctg aaatgagcga
     3421 gcgttttaag ctcaacgacg ttgaagatat tctgccggag tcattgccgc tcggcattcg
     3481 ccagcggctt tcgctggcgg tggcggtgat tcatcgcccg gagatgttaa tcctcgatga
     3541 gcctacttct ggtgtcgatc cggtggcgag ggatatgttc tggcagttga tggtcgatct
     3601 ctcgcgccag gacaaagtga ctatcttcat ctccacccac ttcatgaacg aagcggaacg
     3661 ttgcgaccgc atctcactga tgcacgccgg aaaagtgctt gccagcggta caccgcagga
     3721 actggttgag aaacgcggag ccgccagtct ggaagaggca tttatcgcct atttgcagga
     3781 agcggcaggg cagagcaacg aagccgaagc gccgcccgtg gtacacgaca ccacccacgc
     3841 gccgcgtcag ggatttagcc tgcgccgtct gtttagctac agccgccgcg aagcgctgga
     3901 actgcgacgc gatccagtac gttcgacgct ggcgctgatg ggaacggtga tcctgatgct
     3961 gataatgggt tacggcatca gtatggatgt ggaaaacctg cgctttgcgg tgctcgaccg
     4021 cgaccagacc gtcagtagcc aggcgtggac actcaacctc tccggttccc gttactttat
     4081 cgaacagccg ccgctcacca gttatgacga gcttgatcgt cggatgcgtg cgggcgatat
     4141 cacggtggcg attgagatcc cgcccaattt cgggcgcgat atcgcgcgtg gtacgcctgt
     4201 ggaactcggc gtctggatcg acggagcgat gcccagtcgt gccgaaacgg taaaaggtta
     4261 cgtgcaggca atgcaccaga gttggcttca ggatgtggcg agccgacaat cgacacccgc
     4321 cagccaaagc gggctgatga acattgagac gcgttatcgc tataacccgg acgtaaaaag
     4381 cctgccagcg attgttccgg cggtgatccc gcttctgctg atgatgatcc cgtcaatgct
     4441 aagcgccctt agcgtggtgc gggaaaaaga gcttgggtcg attatcaacc tttacgtgac
     4501 ccccaccacg cgtagtgaat ttttgcttgg taaacagttg ccatacatcg cgctggggat
     4561 gctgaacttt ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg
     4621 cagtttcctg acgctcaccc tggcggcgct gctgtatatc atcattgcca ccggaatggg
     4681 gctgctgatc tccaccttta tgaaaagcca gattgccgcc attttcggaa cggcgattat
     4741 cacattgatc ccagcgacac agttttccgg gatgatcgat ccggtagctt cgctggaagg
     4801 gcctggacgt tggatcggcg aggtttaccc gaccagtcat tttctgacta tcgcccgcgg
     4861 aacgttctcg aaagcgctgg atctgactga tttgtggcaa ctttttatcc cgttgctgat
     4921 agccatcccg ctggtgatgg gcttaagtat cctgctgctg aaaaaacagg agggatgatg
     4981 cgccatttac gcaatatttt taatctgggt atcaaagagt tgcgcagtct gctcggtgat
     5041 aaagcgatgc tgacgctgat tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc
     5101 gttacgccag gatcgttgaa cctcgcgccg atcgccattg ccgatatgga tcaatcgcag
     5161 ttatcgaacc ggatcgttaa cagcttctat cgcccgtggt ttttgccacc ggagatgatc
     5221 accgccgatg agatggatgc cggactggac gccggacgct ataccttcgc gataaatatt
     5281 ccgcctaatt ttcagcgtga tgtcctcgcc ggacgccagc cagatattca ggtgaacgtc
     5341 gatgccacgc gcatgagcca ggcatttacc ggcaatgggt atatccagaa tattatcaac
     5401 ggtgaagtga acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg
     5461 gaaacccgga tgcgctttaa cccgaacctc gatcccgcgt ggtttggcgg ggtgatggcg
     5521 atcatcaaca acattaccat gctggcgatt gtattgaccg gatcggcgct gatccgcgag
     5581 cgtgaacacg gcacggtgga acacttactg gtgatgccga taacgccgtt tgagatcatg
     5641 atggcgaaga tctggtcgat ggggctggtg gtgctggtgg tatcgggatt atcgctggtg
     5701 ctgatggtga aaggtgtact gggcgtaccg attgaaggct cgatcccgct gtttatgctg
     5761 ggcgtggcgc tcagtctgtt tgccaccacg tcaatcggca tttttatggg gacgatagcg
     5821 cgttcaatgc cgcaactggg gctgctggtg attctggtgc tgctgccgct gcaaatgctt
     5881 tccggtggat ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc
     5941 atgccgacga cacactttgt tagcctcgcg caggccattc tctaccgggg tgccggattc
     6001 gaaatcgtct ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt tttcaccatt
     6061 gcgctgctgc gattcaggaa gacgattggg acaatggcgt aaggaaggga aacttgactt
     6121 catactgtga tgtcatacta ttgatgtcat aaagtgaagt cataagaaca tgccatgaga
     6181 acatgccatg agaacatgcc atgagaacat gccatgagaa catgccatga gaaccaaggt
     6241 acaggcttta cggaagaaac aaaaaaatac attggatcaa atatttaaaa ctcctgttcc
     6301 tcaagggatt aagtggtctg atatagagtc actggttaaa gcattaggcg gagaaattaa
     6361 ggaaggaaga ggttcgcgtt gtaagttcat actaaatatg agcgttgcgt gtttccatcg
     6421 gcctcatccg tcgccagata ccgataaagg cgctgtagaa agcgtgcgtg actggttact
     6481 aagtataggg gtaaaaccat gatcaaactg aagacgccaa actctatgga gattgccggg
     6541 caacctgcag ttatcactta cgtgccagaa ctgaatgcat ttcgtggcaa attcctgagc
     6601 ttgtcaggtt attgtgattt tgtctctgac agcattcagg ggctgcaaaa agaaggggaa
     6661 ctttccctgc gagagtatct tgaggattgt aaagctgcgg gtattgagcc gtatgcgcgt
     6721 acagaaaaga ttaagacctt tacgttacgc tatccagaat cgctgagtga acgccttaat
     6781 aatgcggctg cgcagcaaca agtctctgtt aatacctata ttattgaaac actgaatgag
     6841 cgtttgaatc atttataaat tttagcggcg tacattacgc agaatatctg cctttacagg
     6901 atctatctat gcaactgaac ttaattttat aaattaagtt cagttaatat cttttctttc
     6961 ctcgtttcag aaataactca atcttccttc ggcaggcact gcaaatgccc gtgccgcacg
     7021 ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt cgcccatatc acctttcagc
     7081 acggcgattt ccagacagtc gtcgtgattg atatgcacat gcagcgtggc gacggagaga
     7141 tcgtggtgat ggtgctgggt ggagacaatg cggctggcta agtcgcgttt ttcgtgttca
     7201 tacacatagg agagcaccgc gaaaccttgc gtgccgtgct gctgagtggc ctcctgggcc
     7261 agggcgctac gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg
     7321 ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt
     7381 tgcgttaaac cttttctgtg gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt
     7441 gtagcacacg tccggcgtca gaggaaaaag ttaatttctc tcccaccacc tgggtttcga
     7501 cgatttgtcc gttgtccatc accatcaccc gctgacagaa gcgttccacc aggcgtaagt
     7561 cgtgggtgat gaacaggcag gcggtgccaa actgttgttg tagctttttc agcaggcgaa
     7621 tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagtagtt
     7681 tcggttcgac cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact
     7741 gcggtgggcg cttgtcgagc acgctgtcgt cgagatcgac cgcattcagc atttcgctgg
     7801 cgcgcgccag ttgttcggat tttttcagcg atagcaggtg tcgcatcggt tcacgcagga
     7861 tctcgcgcac ggttttgcgc ggattcacgg cactgatgga gtcctgaaac accatctgaa
     7921 tatcacggcg gaaggcttta cgttgggcgc gattaagttt cgccagcgat tcgccgcgcc
     7981 agctaatatt cccttgtgag ggcgattcta agcctaccag cagccgcgcg agagtgcttt
     8041 ttccgcagcc gctgcgcccc agcagggcga cggtttcacc gcttttcagg gtcagcgtaa
     8101 cgttattaag caccgcctga tgctggtgct taccgctaaa tccaccgtgc gcatagtgat
     8161 gggaaaggcc ggagacgtta agtaaagtca tgatgccagc tccataccgt agagggcgag
     8221 atgagcggaa acaaggctgc gcgtcaccgc atgtttgggg gcgttaaaca gcgtttcgac
     8281 atcgccctgt tcgacaatct taccgtgcga catcaccgcc acgtcatccg ccagacgcgc
     8341 cactacgccc atatcatggg tgaccagcag catccccggt gcctgttttt gcataatgct
     8401 ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg ttggttcatc
     8461 ggcgatgata aacggtgatt cgcaaagcac cgccatcgca atcatcatgc gctgcaacat
     8521 gccgccgctc atctcgaacg ggtacagctt cagcacgcgt gcggcgtttt ccagccccac
     8581 cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt ttccccagcg ccaggcaggt
     8641 ttcacgcgcg tgggtgtgca tggtgcgtag cggattaaag gcgctgcgcg ggttctgcat
     8701 gatggtggca attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa
     8761 aatttccccc gccgtctggc gaacgccagc gggcagaatg cccagcgtcg cggcgcaggt
     8821 cagtgatttc ccgctgccgc taccgccgac taacgccagc acacgcccgc gtttaagggt
     8881 taacgatacg ccgtgcacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat
     8941 ctgttgcggc attagtgtgc gtgctccgtc accagatgag ggtccagatg atcgcgcagt
     9001 gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac
     9061 atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt taatcatcac cccccattcg
     9121 gcggtcggtg cggtcacgcc gaggccgagg aaggacatcc ctgcgacgtg cagcatcata
     9181 tggccgatat ccagcgttgc cagcaccagc agcgagggga tcaccgcacc tgccagatga
     9241 tctacaaaca ctcgcacatg gcccgcgcca gaaagccgtg atgccagcac aaactcgcgc
     9301 tggcgcagtg aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg
     9361 gcgatggcga taattacatt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac
     9421 agaatcgagg tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg
     9481 cgcccgccaa tcaacccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc
     9541 aggcaggcca ttaccgaacc gagcgacacg cgggtcgctg ccatcagccg cgagaaaata
     9601 tcgcgaccta agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcgcgac
     9661 ggcaaatcaa tcgcctgggg ggcatacggc agccaccact ggctggtgag cgcaatcagc
     9721 gccagcaggg cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag
     9781 ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat
     9841 cgacaatcaa attacagacc acaaaaacca ccaccatcat cagcgtaaag cactgaatca
     9901 ccggatagtc acggttaaaa atcgccgaca ccgcatagcg cccgacgccc ggccaggcaa
     9961 agatgttttc gataatcatc gtcccgccga tcagttcgcc gatgtgcatc cccacggcgg
    10021 tgatcatcgg cagcgaggca ttacgcagaa tatggcggcg ttcggtctgt ttgtcgttca
    10081 ggccgcgcag acgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc
    10141 gcagtaaacg cgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga
    10201 tgtgctgcca gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca
    10261 tcaccagtaa aaacgccagc cagaagttag gcatcgacac gccaagaaac gcgataaagc
    10321 gcacggcgaa atccggcaaa cgatcgcggt ggcgcgccgc ccagataccg agcggtacgg
    10381 aagtgagcag aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt
    10441 tcagcatatc gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca
    10501 gcgccttcca caaccaggta ccgtactgga cgtacagcgg ctgatccagc ccgagcatgg
    10561 tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt agataaacgc agataatcga
    10621 gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca
    10681 ccatcgggat cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg
    10741 tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg taggggatat tacccagctc
    10801 cggttttgat accaccatca tcgagatgta gctgataggc agataaaccg cctcgtcatg
    10861 cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag
    10921 cacttcgccg atctctttat caatcagcgg cttatcggct aatccttgct gtgcctggaa
    10981 gtcagcgtgc gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc
    11041 ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct
    11101 ctcttcttcg ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat
    11161 ttccgccatc gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg
    11221 ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag
    11281 caacgctttc gctttctgcg gatcgtactg gcgcggtttc aggccgagat tagcgtaagg
    11341 cacagaaggg gcaaacaggg tgtcggcgac ctgctgggtg ccatataacg cgttatcaat
    11401 cagcgatttt ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg
    11461 ggctttggcg gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg
    11521 gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc
    11581 gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat
    11641 gacgttaaag gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac
    11701 gaagacatcg tactgattca gtttcgattc ctgcaaaatc catggtccgg tgccaatcgg
    11761 cgctttaatt ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa
    11821 aggacggggc agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg
    11881 cagctctgtt ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc
    11941 gtgacgttga cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa
    12001 cggttcaccg ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc
    12061 ttctgaatga gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt
    12121 caccaatggt tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg
    12181 gtttagtggc ccgacattca ccggccaggc ggtagtgatt tcatctggtg cagcggcatg
    12241 gacgataaaa gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat
    12301 ggtaacccca atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt
    12361 catactgatt acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact
    12421 gaatcgatcc attgcacact gtcggcggtg agcgtaaagg gagtaggggt gcagatcgcc
    12481 aggcttaaat tttcaagctg acaatggctg actgacagcg aggagtgata ggtgctgtct
    12541 acgctgacga tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc
    12601 cagatgcgcc agaacatttc cagttgctga tcaggatgca cggcgtccat ctcagcgtgt
    12661 tccccgaggc tgaatacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact
    12721 tcgatatcgc agccgacttc gccttcatca ctcaacagca gggcgatatc gtcaccgcta
    12781 tggcttaagt tgaaccatag cggcatttcc ggcgcaaatg caggtttgcc ttgttcgcca
    12841 tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag
    12901 cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg
    12961 cttaaggtcg aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga
    13021 aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg
    13081 cccggcccca gtaaaatcgc cagtttgctg ccacctttcg tcgtttccat ccagatttta
    13141 cacacgctgg ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccaa
    13201 attaacaatg aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt
    13261 ggttctaaaa tattgccgat gaccatatgg accactaaaa acaatgcgcc gaccagaata
    13321 cattcgtaaa caccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc
    13381 gcgccgatat tgggcacgta gttgagcaaa aacgccagta ctgcccacat cagcgcaaac
    13441 tgcaccccca tcagctccag ccccagccag acgatgacac ctgtccataa actgagtagc
    13501 gtcttcaatg caagatagtg cgaaacgcct ttaagtgcgc ggtgtaatcc tgcgatgtga
    13561 atctgtgggt tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc
    13621 ataaaaacta cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc
    13681 attagcgctg tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc
    13741 ggcgacatat gcaaattaag aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc
    13801 aactccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
    13861 ccgaccagcg cagttagtgc gatcagcatc accaccacca caatcgtaat ggcaatgggg
    13921 cgttgtactc cccgacgaat aaaccaggtg acgagcgggt tgaggacgat ggcaaaaaac
    13981 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
    14041 accagcgagg ccagcttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
    14101 attggggctt ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc
    14161 tgattcttac tgctgatttt cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa
    14221 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
    14281 tttgcgcatt gtctctatag tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
    14341 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgcct tctgggcagg
    14401 attggttatc agcctgcaat atttcgccac cttgctgagc cgccctcatg ccggacgtta
    14461 cgccgatttg ctgggaccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
    14521 cggtctgggg tatctgacgg caggattaac cgccagtctg cctgtcatca gcctgttatt
    14581 actttgcctg gggcgcgtca tccttgggat tgggcaaagt tttgccggaa cgggatcgac
    14641 cctatggggc gttggcgtgg ttggctcgct gcatatcggg cgggtgattt cgtggaacgg
    14701 cattgtcact tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg
    14761 gggcggcttg caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt
    14821 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
    14881 gcttgggcgc gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt
    14941 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
    15001 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
    15061 ccgtatcggt ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
    15121 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
    15181 cgggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
    15241 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
    15301 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
    15361 ggcgggactg gtcacaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
    15421 acacgtccct gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga
    15481 tgttttctgc ggtggtctgc gctttttgct gatcgtcagc tggcagagta atttgcatgg
    15541 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
    15601 cggagataat actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg
    15661 ttaccacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca
    15721 ccgccagatc ttcttttgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
    15781 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
    15841 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
    15901 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
    15961 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
    16021 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
    16081 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca
    16141 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
    16201 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
    16261 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
    16321 tcggtgccag ccaccagcgg cgactttgac gcaggcggtt aaaaacgtgc acgtcgagga
    16381 tttgccccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
    16441 agtgggcgag tgcgccgaat ccctgccatg aacccatata gaacagcgac gagatgacat
    16501 aggagattaa taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc
    16561 caaaaatacg cacggtcagg tcggtcgcga gaaagataaa cggaaaacta aacgcgcccc
    16621 aggtggtatg aaaaccaaaa atggagaccg gaagctggac cagatagtta ctggaggtga
    16681 tcaccagcag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
    16741 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
    16801 cacgtctgtt atcttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
    16861 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac
    16921 ggactcacag ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc
    16981 tcttttccag ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga
    17041 tgatggtgcg caaaaccgtg cgcaatatgc agcctggcga aactttactg attatcgccg
    17101 acgatccggc gacgacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
    17161 ttgcgaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg
    17221 gaaagattgg cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga
    17281 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta
    17341 acgcattcgc tgttaccagc acggtcgcac cggtatctgc cagcaccgcc agccatagtc
    17401 cggtcatccc taacagcgtg gtgacgagga agatcccttt cagccccagc gcaatagtga
    17461 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc
    17521 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccactgc
    17581 ccattgcaat cccaatggcg gtagctttca tcgctggcgc gtcgttaata ccatcaccga
    17641 ccatcgccag gggcgcatgt tgattcagct tggtcaccgc tttgacttta tcttccggca
    17701 ataggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
    17761 tatcgccggt gaggatcact cctttaaccc ccagcgcgtt cagttcactg atggcagttg
    17821 ctgcgtcggc gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta
    17881 ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat
    17941 cagcgggatg tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa
    18001 tgccagaccc gaccagcgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct
    18061 gtgcttcgcg tacgatggcc tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
    18121 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    18181 gtttaccgac ggtcagcgta ccggttttat caaacgccac ctgagtaaca cgacccagct
    18241 gttccagcgc cgctccgcct ttaatcaacg ccccacgacg cgctgccgcc gccagcccgg
    18301 aggtaatcgc cgcaggcgtt gagataacta acgcacacgg gcagccaatc agcagcagcg
    18361 tcagcccttt ataaatccac tcctgccagc tggcggcaaa cagcagcggt ggcaccagcg
    18421 tcaccagcag agcgacggcc ataatcgcgg gcgtatagat acggctgaaa cggtcgataa
    18481 accgctcaat gggtgcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    18541 tggcgctggc acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg
    18601 ctcctgcagg aactttatcg cccgttgcgc gctccaccgg aatagattcg ccggtcaggg
    18661 cgctttcatc aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgtccac
    18721 ctgcggcgac ttcaatcaca tcgccagggc gcaggctgtt aatcgccacc tcttcccgct
    18781 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctgacccctt
    18841 gacgtgcgcg gctggcggcc cagccttcca gtcgttcgcc aatcaaaaac agcagcaaca
    18901 ccatcgcagc ttcagccgtt gcgccaataa acagcgcacc aatagcggct acgctcatta
    18961 acgtttcaat ggcgaagtag ctgccggatt tgatcaaccg taatgcctga cgagcaatcg
    19021 ggtacagccc aaccagcgtg gtcgcgataa acgccagttg cccgaacgga tgattgaact
    19081 gctccagacc ccagctgatt gccatcatca cgattagcgt aatcagcgac agattctctt
    19141 tcaggcgtga tgcttgcggt tcttcggcgg cctgttcatc gcgcagggaa tagcctgctt
    19201 tttgcaccgc agattcaact tgtgcacgaa tgtcattgtc ggcatcgacc accagttttt
    19261 cggtggcgaa caacacctgc acctgattca cgcctgcaag ctggcgcaca gcattttcta
    19321 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    19381 agacgttttc agagagcgtt ggcgagctgg aacatgcgcc gtcgcagcaa cagtcgttgg
    19441 cgttctgtac cgcagttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
    19501 tgtcaggagt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    19561 tctggagtcg actccagagt caagttttat cagagataca gcgagcggac gatgaggaag
    19621 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
    19681 tggctcccca gccagacaaa gttactgata aacagcaacg aagcgccaac aaacgcagag
    19741 agcgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    19801 gtcatgccga taaacgtgca gataggccaa cggtactctt ccagccgcgt ccagataatc
    19861 gccagtagca gcgcacccag aaccagcaac accagtggca gaggccagaa gaaagagagc
    19921 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    19981 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    20041 agccctgcca gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac
    20101 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
    20161 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    20221 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    20281 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttatt tccgcatttt
    20341 ccgatgaggg ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat
    20401 tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
    20461 ggctccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
    20521 tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
    20581 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgaggccc agcagtacca
    20641 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgtcagctt
    20701 tgtaggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    20761 aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt
    20821 cggtaaagtg gatttcaata gcgccagttg cataccatgc atcagcacgg taaaaatcag
    20881 cgccacgtta acgaaaatat tcagcgggcg cgggaaggga tgcaccagat tgaggattaa
    20941 aaatccccaa acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    21001 gtgcttcgcg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    21061 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    21121 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    21181 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg
    21241 acattgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    21301 ttaactgctg agaaactgcg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg
    21361 ataacgcttc cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa
    21421 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    21481 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    21541 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    21601 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    21661 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aaccgcgagg agtgtagtcg
    21721 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    21781 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    21841 caggcgcaag agcctgcgaa ggcttctgaa caagccgtcg aagagcaacc gcaggcgcat
    21901 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct
    21961 gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac agccggaacc ggtcgtagaa
    22021 gaaacggcgg agccagtggc tatcgaacgt gaagagctgc cgttgccaga agacgtcaac
    22081 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    22141 gcggcagaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    22201 ctggctgccg aagcggcaga agaggcggtg atggtagttc ctccggcaga agaagagcag
    22261 ccggtggaag aaattgccca ggagcaagaa aaaccgacca aagaaggttt cttcgcgcgc
    22321 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
    22381 cgcggtaaaa aaatcgacga tgatctgttt gaggagctgg aagaacagct gttgatcgcc
    22441 gatgtgggcg tggaaaccac acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc
    22501 aagcagcttc gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt
    22561 ctggcgaaag tcgatgagcc gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg
    22621 gtgggcgtca acggtgtggg taaaactacg acgattggta agctggcacg tcagtttgag
    22681 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc agcggttgaa
    22741 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
    22801 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
    22861 ctgattgccg ataccgccgg acgtctgcag aacaaatcgc atctgatgga agagttgaag
    22921 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
    22981 attgatgcca gcaccgggca gaacgcggta agccaggcca aactgttcca cgaagctgtt
    23041 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
    23101 tcggtggctg accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag
    23161 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
    23221 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
    23281 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
    23341 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
    23401 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc
    23461 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
    23521 tggcgatccc gctgattatc gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
    23581 cggcactgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttccg
    23641 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc
    23701 tggcggacga accgaccggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
    23761 ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc aacgcacgac atcaacctga
    23821 tttcacggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    23881 gccatgaata agcgcgatgc aatcaatcat attcggcagt ttggtgggcg tctggatcgc
    23941 ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta acgcaccgaa acgcgcgaaa
    24001 tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc
    24061 ttccacggcg cattgcagga tctgaaaagc aaactgttcg ccacgttttt aacggtgatg
    24121 gttatcgcca tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaat
    24181 caggcggcga cgcagtatta tccgtcaccg caaatcactg tttacttgca aaaaacgctg
    24241 gacgatgacg ccgctgcggg cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa
    24301 gtgaactatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    24361 ggtgcgctgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    24421 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    24481 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    24541 ctggtcgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    24601 atcggcaaca gcgtacgtct gagcatcttt gctcgccgtg actccatcaa cgtgcagaaa
    24661 ctgattggtg cgacggatgg tttcatcctg cgcccgttcc tgtacggtgg cgcattgctg
    24721 gggttttctg gcgcattgtt gtcactaatt ttgtcagaaa ttctggtact gcgattgtca
    24781 tcggcggttg cggaagtggc acaggttttc ggaacgaagt ttgatatcaa tggtttatcg
    24841 ttcgatgaat gcctgctatt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    24901 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    24961 tttccctgca atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc
    25021 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    25081 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    25141 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    25201 gcaaagttta gctttagccc cagttggcaa cctagattcc tacatccggg cagctaacgc
    25261 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    25321 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    25381 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    25441 cctgatgaaa gcagtgcgcc gtttcaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    25501 cgttcactgg atcaaagcag agatccacga atacgttctg cgtaactggc gtatcgtcaa
    25561 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    25621 tctgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    25681 caaagacgtt cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    25741 ttccgacgac gattccgaca gccagccgat ggcaccggtg ctctatctgc aggataaatc
    25801 atctaacttt gccgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct
    25861 gaccgacgcg atgcaaggtc tggacgagcg tagccaggac atcatccgcg cgcgctggct
    25921 ggacgaagac aacaagtcca cgttgcagga actggctgat cgttacggcg tttccgctga
    25981 gcgtgtgcgc cagctggaaa agaacgcgat gaaaaaattg cgtgctgcta tagaagcgta
    26041 atttccgcta ttaagcagag aaccctggat gcgagtccgg ggtttttgtt ttttgggcct
    26101 ctgtaataat caatttcccc tccggcaaaa cgccaatccc cacgcagatt gttaataaac
    26161 tgtcaaaata gctattccaa tatcataaaa atcgggtatg ttttagcaga gtatgctgct
    26221 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aacaacaaca tcacaacaca
    26281 cgtaataacc agaagaatgg ggattctcag gatgaacata aagggtaaag cgttactggc
    26341 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    26401 cgtgggcgca atgtccggtc cggtggccca gtacggtgac caggagttta ccggcgcaga
    26461 gcaggcggtt gcggatatca acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt
    26521 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    26581 cgacggcatt aaatacgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    26641 catctacgaa gacgaaggca ttttaatgat cacccctgcg gcaaccgcgc cggagttgac
    26701 cgcgcgcggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    26761 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    26821 acagcaatac ggcgaaggtc tggcgcgagc ggtgcaggac ggcctgaaga aaggcaatgc
    26881 taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa gatttctcaa cgctggtggc
    26941 gcgtctgaaa aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg
    27001 gcaaatcctg cgtcaggcac gcgcggcagg gctgaaaact cagtttatgg ggccggaagg
    27061 tgtggctaac gtttcgctgt ctaacattgc gggcgaatca gcggaagggc tgctggtgac
    27121 caagccgaag aactatgatc aggttccggc gaacaaaccc attgttgacg ccatcaaagc
    27181 gaaaaaacag gacccaagcg gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt
    27241 gcaggcgggc ctgaatcagt ctgacgatcc ggctgaaatc gccaaatacc tgaaagcgaa
    27301 ctccgtggat accgtaatgg gaccgctgac gtgggatgag aaaggcgatc tgaaaggctt
    27361 tgagttcggc gtatttgact ggcacgccaa cggcacggcg accgatgcga agtaatcatt
    27421 aatcggcaac tttgggttgc cgccaaattg ctaatatcga gtacgttgct tcatgccgga
    27481 tgcggcgtaa acgccttatc cggcctacaa gatccaaaga aatcagtaaa ttgcaacaca
    27541 cattgtaggc ctgataagcg tagcgcatca ggcaatttct cgttggtcat cacttttgaa
    27601 atcaacttga cgattaacgc ttctcccagc cgccctgttg cgccgtaaat cccagtgcct
    27661 gcataaacgc cgtcatcaca ccgcgatctt ccacgcctgc atccgccatc caccagcatg
    27721 aaacgccagg attgttacgc aaaacctctt ccagcagata ttgccccaca ccgcgacgtc
    27781 gggtgacttc ccgcacacgc agggaatcca gtgctccctc ggtgccgctt aaggttaccc
    27841 gtacggcagc gagcaggcgc tcgttaaaac gcgcggcgta gatacggtgg ttatcgtcaa
    27901 cctgtaaaga ggaaggggaa tactccggcc agatcttttg caggtcaatc cggtcctggt
    27961 cgctaaagtt ttctaatcga atgatggtca gcttcatggg taacccgtgt aaatcaca
//
LOCUS       DAZFAH010000041        27777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFAH010000041 DAZFAH010000000
VERSION     DAZFAH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.041"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1638
                     /gene="yqiK"
                     /locus_tag="ACHJWB_001685"
     CDS             <1..1638
                     /gene="yqiK"
                     /locus_tag="ACHJWB_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO4313336.1"
                     /translation="PSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKV
                     VMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVE
                     GIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTV
                     AEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQD
                     VEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQ
                     TRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSE
                     QQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRA
                     KAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQA
                     LPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASAGSVSGGNLAEQALSAALSYRT
                     QAPLIDSLLNEIGVSGGSLAALTSPLTSSTPVEEKAE"
     gene            1876..1935
                     /locus_tag="ACHJWB_001686"
     CDS             1876..1935
                     /locus_tag="ACHJWB_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4313337.1"
                     /translation="MMKLVIILVVLLLVSFAAY"
     gene            complement(2055..3488)
                     /gene="hldE"
                     /locus_tag="ACHJWB_001687"
     CDS             complement(2055..3488)
                     /gene="hldE"
                     /locus_tag="ACHJWB_001687"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4313338.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIVSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3536..6376)
                     /gene="glnE"
                     /locus_tag="ACHJWB_001688"
     CDS             complement(3536..6376)
                     /gene="glnE"
                     /locus_tag="ACHJWB_001688"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4313339.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(6399..7700)
                     /gene="ygiF"
                     /locus_tag="ACHJWB_001689"
     CDS             complement(6399..7700)
                     /gene="ygiF"
                     /locus_tag="ACHJWB_001689"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4313340.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSKTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7942..8562
                     /gene="ygiM"
                     /locus_tag="ACHJWB_001690"
     CDS             7942..8562
                     /gene="ygiM"
                     /locus_tag="ACHJWB_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4313341.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            8626..9864
                     /gene="cca"
                     /locus_tag="ACHJWB_001691"
     CDS             8626..9864
                     /gene="cca"
                     /locus_tag="ACHJWB_001691"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4313342.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10027..10848)
                     /gene="bacA"
                     /locus_tag="ACHJWB_001692"
     CDS             complement(10027..10848)
                     /gene="bacA"
                     /locus_tag="ACHJWB_001692"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4313343.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10939..11307)
                     /gene="folB"
                     /locus_tag="ACHJWB_001693"
     CDS             complement(10939..11307)
                     /gene="folB"
                     /locus_tag="ACHJWB_001693"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4313344.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     VRAANVGVIIERGNNLKENN"
     gene            11412..12029
                     /gene="plsY"
                     /locus_tag="ACHJWB_001694"
     CDS             11412..12029
                     /gene="plsY"
                     /locus_tag="ACHJWB_001694"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4313345.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12042..12974)
                     /gene="ttdR"
                     /locus_tag="ACHJWB_001695"
                     /gene_synonym="dan"
     CDS             complement(12042..12974)
                     /gene="ttdR"
                     /locus_tag="ACHJWB_001695"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4313346.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     GPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13181..14092
                     /gene="ttdA"
                     /locus_tag="ACHJWB_001696"
     CDS             13181..14092
                     /gene="ttdA"
                     /locus_tag="ACHJWB_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4313347.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTSNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14089..14694
                     /gene="ttdB"
                     /locus_tag="ACHJWB_001697"
     CDS             14089..14694
                     /gene="ttdB"
                     /locus_tag="ACHJWB_001697"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4313348.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14743..16206
                     /gene="ttdT"
                     /locus_tag="ACHJWB_001698"
     CDS             14743..16206
                     /gene="ttdT"
                     /locus_tag="ACHJWB_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4313349.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYMLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16249..17262)
                     /gene="tsaD"
                     /locus_tag="ACHJWB_001699"
     CDS             complement(16249..17262)
                     /gene="tsaD"
                     /locus_tag="ACHJWB_001699"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4313350.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17499..17714
                     /gene="rpsU"
                     /locus_tag="ACHJWB_001700"
     CDS             17499..17714
                     /gene="rpsU"
                     /locus_tag="ACHJWB_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4313351.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17825..19570
                     /gene="dnaG"
                     /locus_tag="ACHJWB_001701"
     CDS             17825..19570
                     /gene="dnaG"
                     /locus_tag="ACHJWB_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4313352.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19765..21606
                     /gene="rpoD"
                     /locus_tag="ACHJWB_001702"
     CDS             19765..21606
                     /gene="rpoD"
                     /locus_tag="ACHJWB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4313353.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21684..22190)
                     /gene="mug"
                     /locus_tag="ACHJWB_001703"
     CDS             complement(21684..22190)
                     /gene="mug"
                     /locus_tag="ACHJWB_001703"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4313354.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKRAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22315..22390
                     /locus_tag="ACHJWB_001704"
     tRNA            22315..22390
                     /locus_tag="ACHJWB_001704"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22348..22350,aa:Ile,seq:cat)
     gene            complement(22489..23334)
                     /locus_tag="ACHJWB_001705"
     CDS             complement(22489..23334)
                     /locus_tag="ACHJWB_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4313355.1"
                     /translation="MSEITVSRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWATKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARETWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKRYPG
                     QLSAI"
     gene            complement(23431..23628)
                     /locus_tag="ACHJWB_001706"
     CDS             complement(23431..23628)
                     /locus_tag="ACHJWB_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4313356.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(23640..24128)
                     /locus_tag="ACHJWB_001707"
     CDS             complement(23640..24128)
                     /locus_tag="ACHJWB_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4313357.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(24125..24499)
                     /locus_tag="ACHJWB_001708"
     CDS             complement(24125..24499)
                     /locus_tag="ACHJWB_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4313358.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(24589..24957)
                     /gene="cbeA"
                     /locus_tag="ACHJWB_001709"
     CDS             complement(24589..24957)
                     /gene="cbeA"
                     /locus_tag="ACHJWB_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4313359.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(25120..25341)
                     /locus_tag="ACHJWB_001710"
     CDS             complement(25120..25341)
                     /locus_tag="ACHJWB_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4313360.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCNGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(25404..25880)
                     /locus_tag="ACHJWB_001711"
     CDS             complement(25404..25880)
                     /locus_tag="ACHJWB_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4313361.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(25896..26381)
                     /locus_tag="ACHJWB_001712"
     CDS             complement(25896..26381)
                     /locus_tag="ACHJWB_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4313362.1"
                     /translation="MKTISHNSSTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(26436..27254)
                     /locus_tag="ACHJWB_001713"
     CDS             complement(26436..27254)
                     /locus_tag="ACHJWB_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4313363.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYSDEHQPVTTADILTPRRWEDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(27354..27587)
                     /locus_tag="ACHJWB_001714"
     CDS             complement(27354..27587)
                     /locus_tag="ACHJWB_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4313364.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(27666..>27777)
                     /locus_tag="ACHJWB_001715"
     CDS             complement(27666..>27777)
                     /locus_tag="ACHJWB_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313365.1"
                     /translation="KATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT         6639 a         7553 c         7198 g         6387 t
ORIGIN      
        1 ccctcctgga tgtttaccgc gattattgcc gtatgcattc tgtttattat tggaattatt
       61 ttcgccaggc tctatcgtcg cgcttcggca gagcaagcat ttgtccgtac cggcttaggt
      121 gggcaaaaag tggtgatgag cggcggcgca atcgtgatgc caatcttcca tgaaattatc
      181 cctatcaata tgaataccct gaaactggaa gtcagtcgct cgacgataga cagcctcata
      241 accaaagatc gtatgcgtgt tgatgtagtc gttgctttct ttgtgcgggt aaaaccttca
      301 gtagaaggga ttgccaccgc tgcccagacg ctggggcaac gcaccctgtc gcctgaagac
      361 ttacgtatgt tggttgaaga taaatttgtc gatgccctgc gtgcaacagc tgcgcaaatg
      421 accatgcatg agttacagga tacccgcgag aacttcgtgc agggggtaca aaataccgta
      481 gctgaagatc tgtcgaaaaa tggcctggaa ctggaaagcg tttcacttac caactttaac
      541 cagacctcga aagaacattt caatccgaac aatgcctttg acgccgaagg tttaaccaaa
      601 ctgactcagg agacggagcg ccgtcgtcgc gaacgtaacg aagttgaaca ggatgtagaa
      661 gttgcggtgc gtgagaaaaa ccgtgatgca ctttcgcgca agttggagat tgaacaacaa
      721 gaagcgttta tgacgcttga gcaggagcag caggttaaaa cccgtactgc tgaacagaat
      781 gctcgtattg cggcttttga agctgaacgt cgccgcgaag cagagcagac gcgaattctg
      841 gctgaacgac agattcagga aacagaaatc gaccgcgaac aagccgtccg ctcaagaaag
      901 gttgaagccg aacgtgaagt tcgcattaaa gagatcgaac aacagcaggt caccgaaatc
      961 gccaaccaga cgaaatcgat cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa
     1021 gcgcgtgcta accttgcact cgcagaagca gtaagcgccc aacaaaacgt tgaaaccact
     1081 cgccagaccg cagaagccga tcgagctaaa caagttgccc taatcgctgc cgcgcaggat
     1141 gcagaaacca aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagag
     1201 atgcaggcgg cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa
     1261 gcagaagcac aacgtgcact gaacgatgct atcaacgtac tttctgatga acaaaccagc
     1321 cttaaattca aactggcatt attgcaggcg ctgcctgcgg taattgagaa atccgtggaa
     1381 ccaatgaagt ctatcgacgg catcaaaatt attcaggttg atgggctgaa tcgtggcggc
     1441 gctgcgggtg atgcgagcgc aggtagcgtt agtggaggaa accttgcaga gcaggcattg
     1501 tctgccgcac tttcttaccg cacacaggca ccgctgattg actccttgct caatgaaatt
     1561 ggcgtttcag gcggctcact ggcggcattg acttcaccct taacctcatc aactcccgtc
     1621 gaagaaaaag cagagtaaac cctcttctat gaataatccc tggcccaaaa gccggggatt
     1681 atttatatct gcgttccgct aaaaggtgca aatgatcagg ctattgcaga attttgcgtg
     1741 acggctcggg gaaggaaaaa ctggctctgg gaaagcattg cgcggggtct gacgctcatc
     1801 aacaatcggg gagtcagcag gggctgaaac gggaaaggcc ctcccgaaga aggggcctta
     1861 tagaaggaaa gggttatgat gaagctcgtc atcatactgg ttgtgttgtt actcgtaagt
     1921 ttcgcagctt attaacagcc aatcagaggg gggagaaatc ctccctcacc cttgttcctt
     1981 tactctaggt tgaaaaaaca acagcgtcaa taggcctgcc atgtacgaag cgagatctgt
     2041 gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgataat gttagtggtc
     2101 gagcaaccgt cttcaaagtt gagcaccaac acttcgccgc cgttggccca gacttcttta
     2161 ctcccggcaa tctcttctgg tttataatca ccgcctttca ccagcagatc tggcaggatc
     2221 ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc gaccgcttcc
     2281 agtgcgccca gcacaatcat acgctgttcg agcgggttta cggggcggga atcccctttc
     2341 agccgtttgg tggaggcatc gctgttcaca gcgacaatca gacggtcacc cagtttgcga
     2401 gcatttgcca gataggagac gtgcccggcg tgcaggatgt caaagacacc gttggtcatc
     2461 accacttttt caccgcgttt acgcgccgcc gctacagcaa gcttcagttc ctcttcggtc
     2521 atcacaccaa agccggtatc tgcgcgccca cgcactgcat tttccagctc gatcggcgaa
     2581 accgtggagg ttcccagttt gccgaccacc acgcctgccg ccgcattggc aaagaagcag
     2641 gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat caccgtgtcg
     2701 cccgcgccgg taacgtcata cacttcctgc gcctgggttg gcatatgcag cggcgcttta
     2761 cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc cgagagttcg
     2821 taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt acatttaccg
     2881 acaacagctt caaattccga gagattcggc gttaacagcg tagcgccgcg gtagcgctca
     2941 aaatcggtac cttttggatc aatcagcacc ggaacgcccg ctttacgcgc cagttggatc
     3001 atctgctgta cgcttgccag agcacctttg gcgtagtcag aaagcaccag cgcgccaatc
     3061 gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc ttcgaaacct
     3121 tcttcaaaat ccaggcggat cagctgttgg ttgcgggaaa gtacccgtaa tttggtgata
     3181 gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc cagagattta
     3241 ctcagcgcac gcgccgcatc gtcaatgccc gtcagcccga ccaggcgagc attggcaccg
     3301 agagaaacga tgttcattgc cacgttagcc gcgccgcccg gacgttcttc gatggtattg
     3361 actttaacca cgggcaccgg cgcttccggc gagatacggc tggtgggacc gtaccagtaa
     3421 cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc tggcagcgtt
     3481 actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc atacttcatt
     3541 cttccaccag ccacttctgc cagcttgccc gcaccagttc acgctctgcg gtgaagcaat
     3601 cctccggcac atggcccggc aattcctgta atgccagatg atgaagttca tcgcgcagcg
     3661 tagtataagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg ttttgcgcca
     3721 gtagttccag aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt
     3781 agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccgcct tcatcagctt
     3841 tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc gcgcattttc tcgcgcattt
     3901 cccgcacttc cgtttgcaga gttttacctt cacgcggcag cgtcataatc tcgcgacgca
     3961 ccgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac tacacgcgca cgcaccagcg
     4021 cctgatgctc ccacgtccag gcctcgtttt tctgataatc agcaaatgct tctgcggatg
     4081 tcaccagcat tcccgccgcc ccggacggac gcagtcgtgc atccacttca tacaaaatgc
     4141 cggaagaggt tcgcgtactg aacagatgca taatgcgttg cgccaaacgc agataaaact
     4201 gtcgcccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa tcatggagga
     4261 agatcaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg ccgtagccga
     4321 ccaccgcaaa accacggcct tcgcgatcgt tgaggtgatt tggcttaccg tagcgggcca
     4381 ccatttgaac ccacgcctgc tgaacgacgg catctatcat agcctctgcc agccaggtta
     4441 agtgatcgct cactttcatc accggtagcg taccagcgat atccgctgcg gcgatgcgta
     4501 acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg tcatcttccg
     4561 gcacgcgcag caaatactgg cgcaactcat cgcggtaggc atcggtcgcc gtcggctggt
     4621 aaagagtgtt tggatcgagc aattcatcca gcaataatgg ataacgcgcc agctggctgg
     4681 caatcatcgg cgatgcggca cacagagaaa tcaaatgttt aagcgccgca gggaattcac
     4741 tgagcaattc taaataggtg gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg
     4801 acagcgtaac ggcagcgtct tcacgcgcac agacatcact tagcagatgt ggcatcagat
     4861 ggtcgagcac ctgacgtcct cgcggcccga tagtacgctt atccagctct ttgcggaaat
     4921 cagcaatcag cgttaggacc tgtttgcgat catcctcgct aagatgcgcc agcactggcg
     4981 tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc gacagcgact
     5041 cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg cgcacattgg
     5101 tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc atcgcccacg
     5161 ccagccgcgc acggttaagc tcatcagaag gaagcgtttg ggtttgttcg tcgttaatgc
     5221 tttgcagcag gttttccaga cgccgcagga acagatatgc cactcgcaat tgttcagcat
     5281 cgttttcaga aagcagatgc agcgcggcaa tgacgctgag cgttggcagt aaagagcgcg
     5341 actgcagcga cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg ataaattcaa
     5401 tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt cgacgcactt
     5461 cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac
     5521 gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg ccttcgctat
     5581 cgcccataat ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc tgctcctggt
     5641 aataatcctc caacgcggca aagctcagca ccagcgggcc actttcgcca aacggacgca
     5701 gccgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccaac actttaatca
     5761 gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccggcgt ccgccctgcg
     5821 tacaaccatg ttccggccag gcaaaaatca gatcgatatc tgaggagaaa ttcagttccc
     5881 caccgcccag cttacccatg cctaaaatca gcagcggttg cgcttcgccc tgcgcattgc
     5941 acggcgttcc ccactcgcgg cagcaagcgt catacagcca gtcacgcgcg gcgacaatca
     6001 gcgtctccgc cagatggctg agctgctgca atatgctctc ttcagtaacc aaagccagcg
     6061 tttgcgccca ggcgatgcgc accataatgc gccgccggaa tagccgcagc tcgcgcatta
     6121 acccggcttc gtcactcaca ttgctgagcg cctcctgcaa ccatgccgcg taatgctgcc
     6181 attcatcagc ctgcggcgat tggctttcca gttccgtcag ccactctgga tgcgcaatca
     6241 cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgcg ctaagtgatt
     6301 tctcggctaa aggctctggc aggcgctcaa caacggtctg ccagtactgc tgtaacggtg
     6361 aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc gctgtgcaac
     6421 cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcaat gcgttgtccg
     6481 gtcgcaatag cgtgacgcag cccctgccag ttctccagcc aggcttgcgc gacgacagga
     6541 tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcgtcagacg tggcaactgg
     6601 tcacggtagc gatcgcccaa cggctggcag aaaacgcttt tcagttcagc ggcatggcgg
     6661 gaaagatgga tatcggcaaa gcgtttgaag gaatcgctca ttttactctg cgctttggca
     6721 tctaaaaacg gctgccaggc tttgcttacc agccattcgg tcaacgccag cttcgccatt
     6781 gccgttttgg tagagtagac cgccgtcacg gcagaaaccg cagaagcgat ggtcgcttcg
     6841 cattgagtca gcaaatcacg taagtgagtg ctcgctttgc gcggcacaat accaccgaac
     6901 agcatcagtg tatgacggac cagaccaatg gctgccagca cctgttcttt cgccgcatcg
     6961 ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacgccag ctcgagcgcc
     7021 gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt ggtcggtttg
     7081 atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt gctcaggctg
     7141 ccctggcgta atccggtttt cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg
     7201 tcgccgctaa gcagttccag ttccagctca cagataggtt cagcaaattc ccccgctttc
     7261 acctccccct gatcgagagc gatttcaatt cggctgtcat cgaccgccac cagccatttt
     7321 tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg aagcgagatc ggcgggcaat
     7381 tcgccgttcg gccagacttc cgtcggtaac tgcgccaggt cgagcgtcgg ttcgctcaac
     7441 gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg
     7501 gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg cccacgcagc
     7561 cagttatccg gcgtttcgta gtaaatattc agcaactgca cagggtcatg gtgctcgccg
     7621 cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac aataaacttt
     7681 aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag tgacgatgaa
     7741 cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa attgccatgc
     7801 aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac acagaggatt
     7861 ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg ttccactttc
     7921 catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac tttactcgca
     7981 cttagcgcca ctgccgtctc acacgctgaa gaaacgcgct atgtttctga tgaactgaat
     8041 acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt taacgccggc
     8101 gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt gaaagacagc
     8161 tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag cctgcgctcc
     8221 cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac caatatcgat
     8281 aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg
     8341 atcaacggat taaaagaaga aaatcagaaa ttgaaaaacg agctgattgt cgcgcagaaa
     8401 aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat catgcaatgg
     8461 tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt actgccacac
     8521 ctgatcccaa cgcgcaaacg caaagatcgc tggatgaact aaatcgcctt cttcgttgca
     8581 ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa gatttatctg
     8641 gtcggtggtg ctgttcggga tgcattgtta gggctaccgg tcaaagacag agattgggtg
     8701 gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt aggccgcgat
     8761 tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg taccgaacgg
     8821 aaatccggtt ccggttacac cggttttact tgctatgccg caccggatgt cacgctggaa
     8881 gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga taacggcgag
     8941 attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc
     9001 cccgcttttg gcgaagatcc gttacgcgtt ctgcgcgtgg cgcgttttgc tgcgcgttat
     9061 gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga gatgacccat
     9121 gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacgga aagcgccctc
     9181 accacccgca atccacaggt gttcttccag gtactgcgcg attgtggcgc actgcgcgtt
     9241 ttattcccgg aaattgacgc actgtttggc gttccggccc ctgccaggtg gcatccggaa
     9301 atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct gagtccgcag
     9361 gtcgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac gccgccagaa
     9421 ctttggccgc gtcatcatgg tcacggtccg gcgggtgtga aattagtgga acaactctgc
     9481 cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc tgagtttcac
     9541 gatctcatcc acaccttccc aatgctgaac ccaaaaacta tcgtcaaatt gtttgattcc
     9601 atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac
     9661 gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa
     9721 gcctgggaag tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg atttaaaggt
     9781 gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag ctggaaggaa
     9841 caacgttgcc caaagcctga ctgagtataa aactaaacgt tagcacgaac cgtcacctcg
     9901 ccccttcggg gagagggtta gggtgagggg aacagaccag caccggtgcg aacaatcacc
     9961 cccaccccgg ccctctcctt acagaccgag ggagaaaacc cggcctgctc ccgaaccttt
    10021 tctcaatcaa aagaacacaa cgtacaccgc agccgccaca ataaagcgat aaatggcgaa
    10081 cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac
    10141 cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg tcaggaagcc
    10201 ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg ccagcaggaa
    10261 cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg aaatggtggc
    10321 cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaacgc
    10381 ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc
    10441 ggcggcaatc agcaacaaac cgccaacgac cagtgcatac atcacattta tcgggttaaa
    10501 caatgactta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa tcatccccag
    10561 caaaatgtgg atcagcgtta aacgaccttt gctttctcct tcgtgctgca acgggcggcc
    10621 aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta ccgccagaat
    10681 tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcaa accccaacaa
    10741 gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc
    10801 gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct
    10861 aaattataaa aatgtaacgc ttgtccgaac tactgcagta tgaccaggtt ataaccgttt
    10921 ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct caatgattac
    10981 gccaacattc gccgcccgca ccactgcccc tggcttgctg agtttgatac gcacccacgg
    11041 cgagttgaag cgtgctaaca gcagctccgc cacctcttca gcaacgcgtt ccaccagcgc
    11101 aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt aactgaggca
    11161 atccgccaca tcatcacttt tcgccgcttt acggttatcc cacgccattt cgatatcgaa
    11221 cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg tgattaccga
    11281 aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct aaccggatac
    11341 cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga attttcaaaa
    11401 cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct
    11461 ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg cgaaccagcg
    11521 gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga gcagccgtag
    11581 cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag
    11641 gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc
    11701 ccgttttctt cggttttaaa ggaggaaagg gcgttgctac cgcttttggt gccatcgcac
    11761 ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga
    11821 gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt tatgtctggt
    11881 ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgtc
    11941 atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca
    12001 aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac ctgatagcct
    12061 tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt
    12121 aatttcatgc tgcgatagag cggcccccgg taaacagccc agatattggc gctttgtgca
    12181 tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac atcccactcg
    12241 gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc gccgctattg
    12301 gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc caactcccat
    12361 attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact taattcctgt
    12421 aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta
    12481 tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat acgaatatcc
    12541 agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa atgcacctgt
    12601 aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct gcgcccaaaa
    12661 ccaaaactac agccaatacg aatcatcccc tccgggcgcg ttttgatttg cgtgacgtca
    12721 tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca
    12781 ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc cagcgtattt
    12841 tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt cgccgctgcg
    12901 gcgctaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt ggctaaaggc
    12961 cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat ttcgcaaaac
    13021 attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat tcacgaactg
    13081 ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg
    13141 cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg aaagtaataa
    13201 gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca tgatttctac
    13261 ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacgtcgtc
    13321 gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga ttgacctgaa
    13381 tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg gttcccgctt
    13441 cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg caaccgtcaa
    13501 agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac
    13561 cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg atgcggaaat
    13621 cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag tgttaatgcc
    13681 gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt
    13741 aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc
    13801 cgtactctcg cgtaaagcca ttttgcgtcc gattggcagc cgccatccca atccaaaagc
    13861 ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg gtccacaagg
    13921 gctgaccagc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc gccatccgtc
    13981 aaccatcggc gttgctgtct ctaccggctg ctgggcgcat cgtcgcggca cgctgctggt
    14041 tcatgccgat ctcacctttg aaaatctgtc tcacaccagg agcgcgttat gaaaaagatc
    14101 ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga tgtgatctac
    14161 ctgaccggta cgctggtgac ctgccgcgac gtttgtcacc gccgtttgat cgaactgaaa
    14221 cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc
    14281 aaaaacggcg acaaatggga gatggtctcc gtcggcccga caaccagtat gcgtatggaa
    14341 agttttgaac gtgaatttat tgagcagacc ggcgtgaaac tggtggtcgg caaaggcgga
    14401 atggggccgc tgaccgaaga aggctgtcag aaattcaagg cgctacatgt gattttccct
    14461 gcaggctgcg cggtgctggc ggcaacccag gtggaagaga ttgaagaagt gcactggaca
    14521 gagctcggaa tgccggagtc gctgtgggtt tgccgggtca aagagttcgg cccgctgatt
    14581 gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa
    14641 cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc
    14701 cggagaggct cgccccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    14761 tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat tccggttccc
    14821 gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
    14881 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc
    14941 atactctctc cctggctgct gttcagcccc gaacagctcg cccagccagg ctttaaattc
    15001 actgcaaaat ccctctcgtg ggcggtttcc ggtttttcta attcggttat ctggctgatt
    15061 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg
    15121 ctgtttctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc ggtgatgttc
    15181 tccgagctga tcctcgcacc tgtaacaccg tccaactcgg cgcgtggtgc ggggattatc
    15241 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
    15301 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    15361 attttcttga cggcgatggc accaaacttg ctgttaattg gactgatgaa aagcgcatct
    15421 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag tattttgctg
    15481 gtgctgctgg ttccctggct ggcttatatg ctgtacccgc cggtactgaa gtctggcgat
    15541 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    15601 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
    15661 atcgatgccg cgatggtcgg ttacagcgtg gtggcactga tgctgcttct gcgcattatc
    15721 agttgggacg acattgtcag taataaagcg gcgtggaacg ttttcttctg gctggcctcg
    15781 cttatcaccc tcgctaccgg actcaacaac actggtttta ttagctggtt tggcaaactg
    15841 ttagcaggca gcttaagcgg ttattcgcca acgatggtga tggtggcgtt gattgtggtg
    15901 ttttatctgc tgcgctactt tttcgccagc gccacggcgt atacctccgc cctcgcaccg
    15961 atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    16021 gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg acccagtccg
    16081 atttactacg gtagtggtta tctgccaacg gtggattact ggcgactggg ggcgattttt
    16141 ggactgatat tcctcgtgtt gctggtgatt accggcttgc tgtggatgcc cgtggtgttg
    16201 ctttaagtga ggacggtgcc ggaaattgct ctccggcacc ggatagtttt acgcagccgg
    16261 taactctgcc agtggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
    16321 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    16381 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tccgccagct tcgcccgtaa
    16441 cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa
    16501 cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    16561 atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt
    16621 cagaccagaa aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa
    16681 gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag gataatccag
    16741 ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc
    16801 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggacac
    16861 cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
    16921 cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca gagaacgccc
    16981 cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    17041 atcaatgtct tttgccgtta agccagactc ctttagcgcc gcctggatca acggtacggt
    17101 tttgcgcacg tgatcgcggg aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa
    17161 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    17221 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    17281 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    17341 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaggc cgcggtgctt
    17401 tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc tggcgttgat
    17461 gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt aaagtacgtg
    17521 aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg
    17581 ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg
    17641 ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca
    17701 ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt gtagttgtaa
    17761 ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct aaaaatcggg
    17821 gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac gcactgacat
    17881 cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg
    17941 ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga aacagtttta
    18001 ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga actacgacaa
    18061 gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg aagtgccatt
    18121 tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc tttatcagtt
    18181 gatggacggt ctgaatacgt tttaccaaca atctttacag caacctgttg ccacgtctgc
    18241 gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct ttgcgattgg
    18301 ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg
    18361 ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca gttacgatcg
    18421 tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga ttggttttgg
    18481 cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa cagacatttt
    18541 ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg ctgaacccaa
    18601 tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat acggcattaa
    18661 ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac tgttgttccg
    18721 cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg atgccgcatg
    18781 gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac gctttatgtt
    18841 tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag aagcgtttga
    18901 agcgcggatg gagcaggcga tgccgctttc cgcatttctg tttaacagcc tgatgccgca
    18961 agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac tgccattgat
    19021 atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag gcaacaaatt
    19081 aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag agagcggcgt
    19141 ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag ggttgctggt
    19201 gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg aaaataagct
    19261 ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc caggtctgac
    19321 caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc ttgaaaaact
    19381 gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca ccgactcact
    19441 caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg
    19501 cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc aggagctggc
    19561 gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc gacagccgca
    19621 ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc ggtaaacaac
    19681 cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac accaacctca
    19741 tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct gaaacttctt
    19801 gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga ccatctgccg
    19861 gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc
    19921 attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga aaacaccgcg
    19981 gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg
    20041 cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga actgttgacc
    20101 cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca ggttcaatgc
    20161 tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga tcgtgttgaa
    20221 gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa cgcagaagaa
    20281 gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga tctggacgat
    20341 gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga caacagcatc
    20401 gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt tgtaacgcgt
    20461 gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat cctgaaactg
    20521 tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc
    20581 atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt
    20641 gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc
    20701 gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa actgcacgat
    20761 gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga agaaaccggc
    20821 ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc
    20881 cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc tatcgccaag
    20941 aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa catcggtctg
    21001 atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac ctacgcaacc
    21061 tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac catccgtatt
    21121 ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa
    21181 gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat gccggaagac
    21241 aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac gccgatcggt
    21301 gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga gctgccgctg
    21361 gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc
    21421 gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg
    21481 ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg
    21541 aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag cttcctggac
    21601 gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc ctctgcacaa
    21661 acgccacctt ttcggtggcg tttttatcgc ccacgcacta ccagcgcctg gtccagctcg
    21721 cgatacgctt caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc
    21781 acccaaatct gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg
    21841 ctgaatccct gttcatatgc tcgtttgccc agaatcgcca acgcctgcgg ctgataatct
    21901 tcaatttttt caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg
    21961 gcttgcaccg ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc
    22021 tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag
    22081 aagcgatttg ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg
    22141 ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct
    22201 ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag
    22261 gcgcacggta atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc
    22321 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgttggttc aaacccaaca
    22381 ggggacacca tacacaacaa agggttagct aataaaccag ctaacccttt ttcttttgcc
    22441 tttgaattgg gtcaggtaat gggtcaggta agcttttttc catctgactc agatggctga
    22501 taattgtccc ggataacgct ttgcaatgat gtcatttagc cgatcaacca gttctggttt
    22561 tctgaatgtg atatgcgcgg agcccttctt aaagtatctg atgctgaaca tctcatcttc
    22621 atagctctct ttaccctgaa cagactgaat gtgatcatcc agacgaatag tgatgttttc
    22681 ccgattgtcg gggattggtt taccgctgaa cagatgcaac attctttcca gatcggcgag
    22741 tcggtcagcc tgttgtccgg tgataagatg aaatccccac cggtcccacc tcactaggtt
    22801 gttgacgata attttactgc caaatttaca ggggcaattg gttttgtaat tccagctcag
    22861 ccctctgaag aggttgatca cacctcgctc aaacacctca tccttattct ggtgcagttg
    22921 ttcaaatgtg ctcaggatgt tcgcttcgct gatttccgga aaattatcgt actccagtga
    22981 ccggtaccat gtctcacggg cctgtgcatc cattaaagaa agcatccctg aacgttgcat
    23041 caggtcacgc cagatttcgc ggtcaagatt ccgggtaatg gctttcattg ctgtttcggg
    23101 tttctccatt agccagcagc cacagcgaaa atcctgtttc gtggcccagt ccagagcagt
    23161 tttacctcca atactttctg tcattgctga tatttcagcc agttgccgga taagtgtgtc
    23221 gatttgcagc agtgtactct ttcgtacagc cagaatgtgg ctgactgagg tggagcagat
    23281 aacgtccgta tgcccgttga ccacttccgg acgggagact gtgatttcag acataatcga
    23341 tattcctttt tgcagataaa tgaaaacgcc agccgtgttc aggctggcgt cagtggaatg
    23401 aagtctggtg agtgagatga tgagtgagac tcaccggttc tggtgctgaa gctgcagttg
    23461 gcgcagggta cggatatcct ctctggcctg ttcgatacag ggcaacagtg ctgctgaatc
    23521 cgttttatcc tcattgttgt caaagataat tcccgattcg cagtcgatat tgtcctgcag
    23581 ccacgcaatc agaatatcca gtgcggtttc cgtggttaat gatttcatgt tgttcctggt
    23641 taccagttga aagtgggtaa acccggcagg cattcagcgc tggcatccag atgaatgaga
    23701 ctgacgccat aacgtcgggt aagtgtgata accagacgac ggaacgtttt tgacagcccc
    23761 aggcgtttaa gccgcagaac cggatatgac cacgcatcgg tacgtaacag atatccgcta
    23821 cctgtgaaat gaatccattc tgattcacca acgtcactgg tctgatgcga cagcgagtac
    23881 agccaggcgt tatccttttc cgtgatatgt gcggtactgc agcgtatgcc ggcaagggtt
    23941 gcaaacggtg gtagtgtgca ggttgactgt tggtcggatt cctccaccac gcggagtgaa
    24001 taaccgtttt cagcgacctt gttaatcagt tcagtgagat taacaccatc gacgtcaacg
    24061 acaatttgcc ccatgttcag tgcctgcaca ttaattctgt cggcttccag catcagggaa
    24121 agtttcatcg tttctccgga tgcttaccca gggtaatgtt atttaccgtt ctgtaattgt
    24181 cgcgggtcat caggccggtc gcccggcgag cccggaggat atcaatactg tttattaact
    24241 gagagcgggg acaagtgctg aatcccggct ggtcggtacg caccagcgcg tatttttcca
    24301 cgagaaaatt caccgcatca cacagtgaaa tgccagcttc aatatgctgc tcaatcacac
    24361 gttcatcggc gaacggtgtg tcattcagtg taaggccgta atgctggccc agtagtcggg
    24421 agagcagagt ctgccagatg gtgacgggag acgggcagct cgatgcctcc cgtacgtgtg
    24481 tatcgggtaa tgttttcata ctgaaggttt tcctgatatg cagagatgaa aaatggaaaa
    24541 gtggcgtggt gaaagcacca ggccggagca gaaggttatt ccggtggttt actttttcgt
    24601 ttcgggcgtc ggataaacag ccagataaac gtaaccaccg ctaccgagag tgtcagcttt
    24661 gcaggtcaga ccttttgcat acagcgtgac ggtatgctga tggcggggat tcagttcacc
    24721 gctggtgagc atgagttcca gttgtttcat cagcagcgga aaggcctggt ccaggtggta
    24781 cgcatctgca tcgctgaaca ggcctctgat accggcgcgg tcggcaaggt aatgcaggcg
    24841 gttaccctcc tgcaccagac gtgccccgaa acagggcgtc acggtgcagg gcagccccca
    24901 ccaggggcgg tcgtgattgt cgtcgggaag cattgtcccg gagagtgcgt ctgacacgat
    24961 aaaatcctca cagaaaatcg gttaagaatg gtttaacgat gacaggtcag ccggcgcgca
    25021 gaccgccttc ggtaaaggtg tattcgttta tgagcagggc ctcatccaca gcggcgtctg
    25081 agctcagcca gtcgtactcg ttttccgggt gacggaacat cagttcaggg tgacgctcat
    25141 cagccggaca tacgggccaa aactgtcctt acgacgttca gcaaacacgg ccagcacacc
    25201 gggaatatcc tgcacttcac gaccggtata cgcttcagtg ctgccgtgcc agcggtattt
    25261 gccgttacag aacgggaaaa gacgggatgt cggatgttgt tggtgaatac gcatggcttc
    25321 accacgagtg ataattttca taatgggata cctctgaaga cagaagataa aagtgaaaac
    25381 aggtgtgatg tggttgtgac gggttaaagc agaccatgtt cggcaaagga gaaaacctgg
    25441 ttgccaccga ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc
    25501 agacgttcgg tgataagccg gtctgcctta ctgggtgtga cttcaccgga cgggtgattg
    25561 tgcgccagca ccacggcagc ggcattgtgg tacagggcgc gtttaatcac ttcccgggga
    25621 tggacttccg tgcggttgat ggtgccggtg aagagggttt caccggcaat cagctgattc
    25681 tggttattca gatacagcac ccggaactct tcacgctcca gtcccgccat gttcagaatc
    25741 agccattccc gtgccgcacg ggtggaggtg aaggccacac cgggttcatg aagatggcgg
    25801 tccagggttt tcagagcccg cagaatgaga ctgcgctcgc cgggcgtcat ttctccgggc
    25861 agaaaggaaa gctgctgcat tgtccctgcc tccattcagt cgatgatgcg cataatggcg
    25921 ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt aataatggac cgtcatggca
    25981 taacactccg tacgacaggc gtgatgactg tacgtcatca gacaggcagc aatgccagcg
    26041 gcttccgggc tcatttcagc gcggttgccg ttcatggcat tgaacagtac ccatgtttcg
    26101 tcatcatcgt catccggttc gggggccata aatgccccgc cgttgttcag agtgtacaga
    26161 ttccagatac caccgcagta gtcttcgcac agacggtcca tccagccgaa aacacggggc
    26221 tccagggtca cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt
    26281 tcatcaggga ctatggtggc aaccaactga ggctggtcat tccctgatgc agcggttacg
    26341 gaaacagaag atgtggagga attatgtgaa atggttttca tgaaatgact ccttaaagga
    26401 caaataaatg cgtggttttc agggtaatgg ctgaatcagc gcaggctctc cagcagggtt
    26461 tccgccatca cccacaatgc gcggttgagc ttaatgtcag tgtcaatgct gtgaatggca
    26521 cgggtatgga tacgttttcc tttagcgctg cgacctgaaa ttccgccttt cagcatattc
    26581 tcctgaatgg tctgataagc actccacagg tccttaccgt aatcctccca gcgtcgtggc
    26641 gtcagaatgt cggcggtggt gacgggctga tgttcgtcac tataacggta agtcagtgcc
    26701 gcctgtgcca gcgcctggcg tgccggtggc ggcagaatca gcgactgcat ggcatcacgc
    26761 ttttcctcaa tccggtcaaa cacgcccacc acctcgtaag ccccttcaat gactctgtcc
    26821 accacatttc cccggtgcgg aacacgcact tcccccagag actgaccaca gacgcatccg
    26881 ttctggcaga cgaacctgaa gtaacccggc agcatctggt agctggaggt accgtcatga
    26941 gagttgagca gaataatttc agggacatgt tctccgttta tctctccggc ccgccgcaga
    27001 cgcagcatgt gttttgtgta tccccggcgg cccgggtcgc gcacacgggt ctggcaggcg
    27061 aagaatggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt ggggatgtac
    27121 gtatagttct ggcttcggga cgtgtgttta tcttccccga aaatccccgg cacatagtgc
    27181 atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggttcgcata accaaaacga
    27241 ctggctagtc gcataatttg ctccttatcg gtggttaaga tttactggtg taataaatga
    27301 aaaagccacg tctcccggag aagacgcggc ctgatgggtg aaatgaatga cgtttattgt
    27361 ctgagaagac cgtcactggc gagccgcgta ttaagcacct ttccggcatc gggttcagaa
    27421 ttccggcagc gccagcgtcg ctgaccttcc gcgttgcgga taacccgacg gaaatgcatt
    27481 cccgggtcat cctggataaa cacattgcgg taagcggtta tgacagcaac cgcctgcccg
    27541 tatgagaaag atccttcagc caggacatac tctgtgtaac ccggcatatc tgttttctcc
    27601 tgataaataa aaagcctctg ccacgcgttg tgacagaggc tgaagcatga aactgacttc
    27661 agggatcagt taacgttttt tccggaaacg gtaatcagca gtggatggta gtcctgggga
    27721 ttgaaaaccg ataacggcag actgacacga tggccgttac tttcttcagt tgcttta
//
LOCUS       DAZFAH010000042        27081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFAH010000042 DAZFAH010000000
VERSION     DAZFAH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.042"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..195
                     /locus_tag="ACHJWB_001716"
     CDS             <1..195
                     /locus_tag="ACHJWB_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313366.1"
                     /translation="PHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLITDYEVQK
                     YLSFQLPVIRYRERFWQYME"
     gene            202..2625
                     /gene="pgaA"
                     /locus_tag="ACHJWB_001717"
     CDS             202..2625
                     /gene="pgaA"
                     /locus_tag="ACHJWB_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO4313367.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2634..4651
                     /gene="pgaB"
                     /locus_tag="ACHJWB_001718"
                     /pseudo
     CDS             2634..4651
                     /gene="pgaB"
                     /locus_tag="ACHJWB_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            4644..5969
                     /gene="pgaC"
                     /locus_tag="ACHJWB_001719"
     CDS             4644..5969
                     /gene="pgaC"
                     /locus_tag="ACHJWB_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO4313368.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5971..6384
                     /gene="pgaD"
                     /locus_tag="ACHJWB_001720"
     CDS             5971..6384
                     /gene="pgaD"
                     /locus_tag="ACHJWB_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO4313369.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6434..7222)
                     /gene="phoH"
                     /locus_tag="ACHJWB_001721"
     CDS             complement(6434..7222)
                     /gene="phoH"
                     /locus_tag="ACHJWB_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4313370.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7842..9113)
                     /gene="efeB"
                     /locus_tag="ACHJWB_001722"
     CDS             complement(7842..9113)
                     /gene="efeB"
                     /locus_tag="ACHJWB_001722"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4313371.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVFASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9119..10246)
                     /gene="efeO"
                     /locus_tag="ACHJWB_001723"
     CDS             complement(9119..10246)
                     /gene="efeO"
                     /locus_tag="ACHJWB_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4313372.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDACEDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10304..11134)
                     /gene="efeU"
                     /locus_tag="ACHJWB_001724"
     CDS             complement(10304..11134)
                     /gene="efeU"
                     /locus_tag="ACHJWB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4313373.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11800..13308)
                     /gene="putP"
                     /locus_tag="ACHJWB_001725"
     CDS             complement(11800..13308)
                     /gene="putP"
                     /locus_tag="ACHJWB_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4313374.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13467..13676)
                     /locus_tag="ACHJWB_001726"
     CDS             complement(13467..13676)
                     /locus_tag="ACHJWB_001726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313375.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13731..17693
                     /gene="putA"
                     /locus_tag="ACHJWB_001727"
     CDS             13731..17693
                     /gene="putA"
                     /locus_tag="ACHJWB_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4313376.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDIAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17733..18371)
                     /gene="rutR"
                     /locus_tag="ACHJWB_001728"
     CDS             complement(17733..18371)
                     /gene="rutR"
                     /locus_tag="ACHJWB_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4313377.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18659..19750
                     /gene="rutA"
                     /locus_tag="ACHJWB_001729"
     CDS             18659..19750
                     /gene="rutA"
                     /locus_tag="ACHJWB_001729"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4313378.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19750..20442
                     /gene="rutB"
                     /locus_tag="ACHJWB_001730"
     CDS             19750..20442
                     /gene="rutB"
                     /locus_tag="ACHJWB_001730"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4313379.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAELFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20454..20840
                     /gene="rutC"
                     /locus_tag="ACHJWB_001731"
     CDS             20454..20840
                     /gene="rutC"
                     /locus_tag="ACHJWB_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4313380.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVDIATIAHIAK"
     gene            20848..21648
                     /gene="rutD"
                     /locus_tag="ACHJWB_001732"
     CDS             20848..21648
                     /gene="rutD"
                     /locus_tag="ACHJWB_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4313381.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21658..22248
                     /gene="rutE"
                     /locus_tag="ACHJWB_001733"
     CDS             21658..22248
                     /gene="rutE"
                     /locus_tag="ACHJWB_001733"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4313382.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22259..22753
                     /gene="rutF"
                     /locus_tag="ACHJWB_001734"
     CDS             22259..22753
                     /gene="rutF"
                     /locus_tag="ACHJWB_001734"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4313383.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22774..24102
                     /gene="rutG"
                     /locus_tag="ACHJWB_001735"
     CDS             22774..24102
                     /gene="rutG"
                     /locus_tag="ACHJWB_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4313384.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMAGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24185..24358)
                     /gene="ymdF"
                     /locus_tag="ACHJWB_001736"
     CDS             complement(24185..24358)
                     /gene="ymdF"
                     /locus_tag="ACHJWB_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4313385.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24731..25327
                     /gene="wrbA"
                     /locus_tag="ACHJWB_001737"
     CDS             24731..25327
                     /gene="wrbA"
                     /locus_tag="ACHJWB_001737"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4313386.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25348..25575
                     /gene="yccJ"
                     /locus_tag="ACHJWB_001738"
     CDS             25348..25575
                     /gene="yccJ"
                     /locus_tag="ACHJWB_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4313387.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25613..26854)
                     /gene="agp"
                     /locus_tag="ACHJWB_001739"
     CDS             complement(25613..26854)
                     /gene="agp"
                     /locus_tag="ACHJWB_001739"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4313388.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTRAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         6563 a         7055 c         6903 g         6560 t
ORIGIN      
        1 cctcatcatt ggaatggatt ttcgggcgag aaaaggattt tatatggaca ctctgctcat
       61 catttcttct tctcatcatc aacaattcac gtctctcttc cgcgtttaat aacggattat
      121 gaggtgcaaa aatatctttc ttttcagtta cctgtaatta gatacagaga gagattttgg
      181 caatacatgg agtaatacag gatgtattca agtagcagaa aaaggtgccc gaaaaccaaa
      241 tgggctttga aacttcttac tgccgcattt ttagcagcga gtcccgcggc gaagagtgct
      301 gttaataacg cctatgatgc attgattatt gaagctcgca agggtaatac tcagccagct
      361 ttgtcatggt ttgcactaaa atcagcactc agcaataacc aaattgctga ctggttacag
      421 attgccttat gggccgggca agataaacag gttattaccg tttacaaccg ctaccgtcat
      481 cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa
      541 tggcaaaact cgcttacact gtggcaaaag gcgctctctc tggagccgca aaataaggat
      601 tatcaacggg gacaaatttt aaccctggca gatgctggtc actatgatac tgcgctggtt
      661 aaacttaagc agcttaactc tggagcaccg gacaaagcca atttactcgc agaagcctat
      721 atctataaac tggcggggcg tcatcaggat gaattacggg cgatgacaga gtcattacct
      781 gaaaatgcat ctacgcaaca atatcccaca gaatacgtgc aggcattacg taataatcaa
      841 cttgctgccg cgattgacga tgccaattta acgccagata ttcgcgctga tattcatgcc
      901 gaactggtca gactgtcgtt tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc
      961 gatcgcgccc tcgcccaata cgctgcatta gaaattctgt ggcacgataa cccagaccgt
     1021 actgcccagt accagcgtat tcaggttgat catcttggcg cgttattaac tcgcgatcgt
     1081 tataaagacg ttatttctca ctatcagcga ttaaaaaaga cggggcaaat tattccgccc
     1141 tgggggcaat attgggttgc atcggcttat ctcaaagatc atcagccgaa aaaagcacag
     1201 tcaataatga ccgagctctt ttatcacaag gagaccattg ccccggattt atccgatgaa
     1261 gaacttgcgg atctctttta cagccacctg gagagtgaaa attatccggg cgcgctaact
     1321 gtcacccaac ataccattaa tacttcgccg cctttccttc ggttaatggg cacgcctacg
     1381 agcatcccga atgatacctg gttacagggg cattcgtttc tctcaaccgt agcaaaatat
     1441 agtaatgatc ttcctcaggc tgaaatgaca gccagagagc ttgcttataa cgcaccagga
     1501 aatcagggac tgcgcattga ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca
     1561 gcagaaaatg aattaaaaaa agcagaagtg atcgagccac gtaatattaa tctggaggtt
     1621 gaacaagcct ggacagcatt aacgttacaa gaatggcagc aggcagctgt cttaacgcac
     1681 gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac gattaaaacg tgcggttgat
     1741 gtacataatc ttgcagagct tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg
     1801 gatagtggta aacatgatgt cgacttaacc accatcgttt attcaccacc gctgaaggat
     1861 aactggcgcg gttttgctgg attcggttat gccgatggac aatttagcga aggaaaaggg
     1921 attgttcgcg actggcttgc gggtgttgag tggcggtcac gtaatatctg gctcgaggca
     1981 gagtacgctg aacgcgtttt caatcatgag cataaacccg gcgcgcgcct gtctggctgg
     2041 tatgatttta atgataactg gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt
     2101 ccattacggg caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg
     2161 tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt tcactgattt ttccgacagt
     2221 aaccagcgtc atgaagtctc acttgagggt caggaacgca tctggtcttc accatatttg
     2281 attgtcgatt tcctacccag tctgtattac gaacaaaata cagaacacga taccccatac
     2341 tacaacccta taaaaacgtt cgatattgtt ccggcatttg aggcaagcca tttgttatgg
     2401 cgaagctatg aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa
     2461 aaacattatg gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat tagctggaat
     2521 gacgtgattg atgctggcgc aacgctacgc tgggaaaaac gaccttatga cggtgacaga
     2581 gaacacaact tatacgttga attcgatatg acattcagat tttaaggata aatatgttac
     2641 gtaatggaaa taaatatctc ctgatgctgg tgagtataat tatgctcacc gcgtgcatta
     2701 gccagtcaag aacatcattt ataccgccac aggatcgcga atctttactc gccgagcaac
     2761 cgtggccgca taatggtttt gtagcgattt catggcataa cgttgaagac gaagctgccg
     2821 accagcgttt tatgtcagtg cggacatcag cactgcgtga acaatttgcc tggctgcgcg
     2881 agaacggtta tcaaccggtc agtattgctc aaattcgtga agcacatcga ggaggaaaac
     2941 cgctaccgga aaaagctgta gtgctgactt ttgatgacgg ctaccagagt ttttataccc
     3001 gcgtcttccc aattcttcag gccttccagt ggcctgctgt atgggccccc gtcggcagtt
     3061 gggtcgatac gccagcggat aaacaagtaa aatttggcga tgagttggtc gatcgagaat
     3121 attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc ccggctcgtt gagctcgctt
     3181 ctcatacatg gaattctcac tacggtattc aggctaatgc caccggcagc ttattgcctg
     3241 tatatgtaaa tcgtgcatat tttactgacc acgcacggta tgaaaccgca gcagaatacc
     3301 gggaaagaat tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg
     3361 taaatccaca cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg atagaggaat
     3421 taaaaaactc ggttatgaca tgttcttcac ccttgaatca ggtttggcaa atgcgtcgca
     3481 attggattcc attccgcggg tattaatcgc caataatccc tcattaaaag agtttgccca
     3541 gcaaattatt accgtacagg aaaaatcacc acaacggata atgcatatcg atcttgatta
     3601 cgtttatgac gaaaacctcc agcaaatgga tcgcaatatt gatgtgctaa ttcagcgggt
     3661 gaaagatatg caaatatcaa ccgtgtattt gcaggcattt gctgatcccg atggtgatgg
     3721 gctggtcaaa gaggtctggt ttccaaatcg tttgctacca atgaaagcag atatttttag
     3781 tcgggttgcc tggcaattac gtacccgctc aggtgtaaac atctatgcgt ggatgccagt
     3841 attaagctgg gatttagatc ccacattaac gcgagtaaaa tacttaccaa caggggagaa
     3901 aaaagcacaa attcatcctg aacaatatca ccgtctctct cctttcgatg acagagtcag
     3961 agcacaagtt ggcatgttat atgaagatct tgccggacat gctgcttttg atggcatatt
     4021 gttccacgat gatgctttgc tttcagatta tgaagatgcc agtgcaccgg ctatcacggc
     4081 ttatcagcaa gcaggcttta gcgggagtct gagcgaaatt cgacaaaacc cggagcaatt
     4141 taaacagtgg gcccgcttta aaagtcgtgc gttaactgac ttcactttag aacttagtgc
     4201 gcgcgtaaaa gccattcgcg gtccacatat taaaactgca cgaaatattt ttgcacttcc
     4261 ggtaatacaa cctgaaagtg aagcctggtt tgcacagaat tatgctgatt tcctaaaaag
     4321 ctatgactgg accgctatta tggctatgcc ttatctggaa ggtgtcgcag aaaaatcggc
     4381 tgaccaatgg ttaatacaat tgaccaatca aattaaaaac atccctcagg ctaaagacaa
     4441 atctatttta gaattacagg cacaaaactg gcagaaaaat ggtcagcatc aggctatttc
     4501 ttcgcaacaa ctcgctcact ggatgagcct attacaactg aatggagtga aaaactatgg
     4561 ttattatccc gacaattttc tgcataacca acctgaaata gaccttattc gtcctgagtt
     4621 ttcaacagcc tggtatccga aaaatgatta atcgcatcgt atcgtttttt atattatgtc
     4681 tggtgttatg cataccccta tgcgtagcgt actttcactc tggtgaactg atgatgaggt
     4741 tcgttttctt ctggccgttt tttatgtcca ttatgtggat tgttggcggc gtctatttct
     4801 gggtctatcg tgaacgccac tggccgtggg gagaaaacgc accagctccc cagttgaaag
     4861 ataatccgtc tatctccatt atcattccct gttttaatga ggagaaaaac gttgaggaaa
     4921 ccatacacgc cgctttagca cagcgttatg agaacattga agttattgcc gtaaatgacg
     4981 gttcaacaga taaaacccgt gccatcctgg atcgcatggc tgcacaaatt ccccatttgc
     5041 gggtcattca tctggcgcaa aaccagggga aagccattgc gcttaaaacc ggagctgccg
     5101 cggcgaaaag tgaatatctg gtgtgcattg atggcgatgc gttattagac cgcgatgcgg
     5161 cggcatatat tgtggaaccg atgttgtaca acccgcgtgt gggtgccgta accggtaatc
     5221 ctcgtattcg aacacgttct accctggtgg gtaaaattca ggttggcgag tattcctcaa
     5281 ttattggttt gatcaagcga acccagcgta tctatggaaa cgtatttacc gtttccggtg
     5341 ttattgccgc atttcgtcgc agcgccctgg cagaagtggg ttactggagt gacgatatga
     5401 tcaccgaaga tattgatatt agctggaagc tgcagttgaa tcagtggacg attttttacg
     5461 agccacgggc gctgtgctgg atattaatgc ctgaaacgtt aaaagggctg tggaaacagc
     5521 gcctgcgctg ggctcagggc ggtgcagaag tattcctcaa aaatatgaca aggttgtggc
     5581 gcaaagaaaa ctttcgaatg tggccgctgt tttttgaata ctgcctgacg acaatatggg
     5641 ccttcacctg cctggtcggt ttcattattt acgcagtcca acttgccggt gtaccgttaa
     5701 atattgaatt gacacatatc gctgcgacac atactgccgg aatattactg tgtacgttat
     5761 gtttactgca atttattgtc agcctgatga tcgagaatcg ctatgagcat aatctgactt
     5821 catcgctttt ctggattatt tggttcccgg ttattttctg gatgctgagc ctggcaacga
     5881 cattggtatc atttacacga gtcatgttga tgcctaaaaa gcaacgcgcc cgttgggtaa
     5941 gtcccgatcg cgggattctg agaggttaat atgaacaatt taattattac gacccgacaa
     6001 tcaccagtac gtttactggt tgattatgtt gccacaacca tcttgtggac attatttgcg
     6061 ttgttcatat tcttattcgc catggatctg ctgacgggtt attactggca aagcgaggcc
     6121 agaagccgac ttcagttcta ttttttgctg gcagtggcga atgccgtcgt gttaattgtc
     6181 tgggcgctgt acaataagct gcgttttcaa aaacagcagc atcatgcagc ctaccaatat
     6241 acgccgcaag aatatgcaga gagcttagca atacctgatg agctctatca gcaactacaa
     6301 aaaagccaca ggatgagcgt acacttcacc agccaggggc aaataaaaat ggttgtttca
     6361 gaaaaagcgc tagtccgggc ataaacaccc aaaacaaagc ccggttcgcc cgggctctgc
     6421 accgataata cacttaactg tatgcatgca gcgtacgttg gcaaagtgcc gaacgtacgc
     6481 agtcctcttt accgaaccgg acgatcccaa ccatttcatc ttcttcgaaa cgttccagcg
     6541 cgtcacttaa tccggagcac acgccgcgag gcaaatcgca ttgcgtgata tcaccgttga
     6601 cgataaccgt cacgttctcc ccgaggcggg ttaaaaacat tttcatttgc gcggcagtca
     6661 cattctgcgc ctcgtcaaga atgacgactg cattttcaaa ggtacgtcca cgcatatagg
     6721 cgaacggcgc aatttccacc ttccctattt ccggtcgcag gcagtactgc ataaaggaag
     6781 cccctaagcg ccggaccagc acgtcgtaga ccgggcgaaa atagggagca aacttttctg
     6841 cgatatctcc aggtaagaag ccaagatctt catcggcttg cagaactgga cgggtgacga
     6901 taatcctgtc gacatcctta tgtatcaggg cttctgccgc ttttgctgcg ctgatccagg
     6961 tttttccgca cccggcttcg cccgtggcga atatcagctg cttactctca atagccttca
     7021 gatagtgcaa ttgcgcttca tttcgcgcga ggatgggcga agtatcgcga ctgtcgcggg
     7081 ccataccaat ggcttctacg ccgcccatct gcacaagcga ggtgaccgat tcttcttcac
     7141 gctgcttatg gctgcgcgaa tcccgtctca gcacacgttt tgcctcgcga cgagctttga
     7201 tcactgcttt ttgtcttccc atggagagca ccttgagttg tttgtattca tcacacgcgc
     7261 cgttggcagc gcgattatgc gcacgaacat cagagggttg gcttccttgt aagccatagt
     7321 ttgcttttgg ataaaatgcc gaaaaacggc tacgcgcacc gtttacggcg tcggtaacac
     7381 atgaaaagaa aggatgaggt tgaaaatgca aagtgacgag atgactaccg gaggagaaaa
     7441 ctccgcgagt ggtggcgcgt tgattatcta aaacatgtcc agtacaggac gttaccatcc
     7501 gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact taataagtac
     7561 aacagatctc gcatttattg caacaatata tttacttata tttaactata aaacaccatt
     7621 tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg aaaaaagctg
     7681 tgtgctttca gcaggatttg catcaggcat cagtgcacga tcgccgtatg cggcgtgaac
     7741 gccttatccg cctaccgaac ccgcaccgat tgtaggcata agaagacgcg caagcatcgc
     7801 atcaggcatc tgcgcaagaa cgccttatcc gtctaaaaac attaaacccg caataacgcg
     7861 cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata accgccgccg
     7921 ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac tgtcaggaag
     7981 cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag ttgcccggag
     8041 ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga ctcactctcc
     8101 gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccggg
     8161 tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc accggtttgc
     8221 ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccact
     8281 cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc aggctcctgc
     8341 tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct atcgggattg
     8401 gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc
     8461 gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc cggcgtgtgt
     8521 ttgatgatat cgcgcagcgc atggataacc gtgtcctggg tgttggcgca aatctgtagc
     8581 aacacatcac catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc
     8641 tgcagctttt ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc
     8701 accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     8761 agtggtggca ggcgcggatt tggcgtttct ggtgctgctc cgccctgagt cagaaaagca
     8821 aaacgctgag tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct ggcaaacaca
     8881 tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca
     8941 ccataaaacg gctgtttctc attgcgagca tccggtgaaa gcgtgcccgg tgcactttgc
     9001 gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc aatccctttc
     9061 agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat ctttatactg cataacgctt
     9121 aatccagtcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggcc
     9181 ctttcagcgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag
     9241 tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccaaca
     9301 gttccgggtt ggctttttgc agttgtggac gcagcagatc gacaattttc tgcgagcctt
     9361 caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctga
     9421 ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc gccgaccact tttgaaggtg
     9481 ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa agctgctcag
     9541 cgtactgatc catccctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga
     9601 aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc atcttcacag gcgtcaatgc
     9661 tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg
     9721 tcggcgcata cagtgctttc gctttttcga tatcgccagc tttaatcgcg tcggtaaagg
     9781 ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa
     9841 ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt
     9901 tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag
     9961 gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca
    10021 ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg ttctgaataa
    10081 tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca
    10141 cggtcacttt gacctgcgtc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg
    10201 cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc
    10261 tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg
    10321 ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa
    10381 accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaatc ccttccatca
    10441 gcgtgccaaa cagcgagtga gttgagagca ccgcactcat atcgaaggcg atttcctgaa
    10501 agtggttcca caatccggct tcatgaaatg cgcgaatggc accagctgcc agccctgcgg
    10561 cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac
    10621 cgccccagta gagcaggaag cctagcacca cggcagtagc aagaccgagc attgcaccca
    10681 gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca
    10741 gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc
    10801 cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt
    10861 tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt
    10921 caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg aagatcccca
    10981 agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca atccatcggc
    11041 ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc
    11101 cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg
    11161 agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggtaaagaa
    11221 aaatacaagc ggaatgttta taaaatgatg ttgaaatgta aacaaagaga ggtaatgcca
    11281 ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt
    11341 ctcgccggat gccgcgtaaa cgccgtatcc agccgacaaa accgtcctga ctcaatacat
    11401 caaaacttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atcctcgatt
    11461 atcaaatcta actttttgcc cggaacataa gaacagcgcg ggtgttggtg gcgtcaaaat
    11521 cgctgaagcg ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgcgtct
    11581 tacaggacgt tacctcgcgc ccgacccggt agccgaaggg gataagctga aggtaccgcg
    11641 cagcggcgat tttgttcgcc agagcccggg ggtgcagggg gccgcggcga ttggccgccc
    11701 cctgcgcgct ccctgcacca ttcaatttat atggcgaggc acattaaggg agcgcaaccc
    11761 gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagctttcct gcaaccgtga
    11821 cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga
    11881 cggcgcttta cccagcaaac taaacactac aatcccgata ctgccgaaga tgaagcccgg
    11941 aataatttcg tagagtccca gccagccgaa ctgcttccag acgataaccg tcagcgcacc
    12001 gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaacag
    12061 caccaccgga ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac
    12121 gcggttttcc gggtttgccg ccagcgcaat cgccaccagc gccaccacca gcaccatcac
    12181 acgtcctacc cacaccagct ctttctggct ggcatgttta cgcagaaacg ctttgtacaa
    12241 atcttcggta atcgcactgg agcacaccag cagctggcag cttaacgttg acattaccgc
    12301 cgccagaatt gccgacagca aaatcccggc aatccacggg ttaaacagaa tttgcgccag
    12361 ttcgataaac acacgttcag cattctggtt taccgcacct gctaccgccg ggtgctcatt
    12421 aaagtaagca ataccaaaga agccgacagc caccgcccct gccaggcaga ggatcatcca
    12481 ggtcatactg atacgacgcg catggacaat gctgtggtga gaatccgccg ccataaaacg
    12541 cgccaggatg tgcggctgcc cgaagtaacc cagcccccag cccatcagcg agataatggc
    12601 aacaaagttc agacctttga gcatatcaac gttttcgatg ctcttttgtt tgatcacttc
    12661 cagcgagtca ccaaagccac cgacactgat aatgacgata accggcgtca ggatcagggc
    12721 aaaaatcatc aggctggcct gtacagtatc agtccagctc accgcgagga aaccgccaat
    12781 aaaggtgtaa aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc
    12841 aaaggtactt tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa
    12901 gaacagcaaa ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaagcg
    12961 cccggtgaaa taatccggca gggttaaggc gttattgttg tattcggtat gcacacgcaa
    13021 ccgcccggcc accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca
    13081 gctttcggaa atcccggaaa gaaaaaccgc gcccggcaac cccattaaca gccagccgct
    13141 catatccgac gcacccgccg ataatgccgt cacgaatggc ccaagactac gaccgcccag
    13201 aatatagtcg tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc
    13261 aaagatatag acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca
    13321 aaaaattatt atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac
    13381 agagtttatg ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg
    13441 atttaacaca ccatttacat taaattttag tgctcagcga cactattttt catcaggttg
    13501 cactctctca cattttttgc ggttgcacct ttcaaaaagg ttaactgccg cagagaaaaa
    13561 gtctgagtta tttttttaat ccctgtcata tcgatttctt ttattaacat ttcattcatt
    13621 tttaagcttg ctacgcaggt cacatttaac atggttgcac aaagttgcaa catcatggat
    13681 atttcacgat aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca
    13741 ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac
    13801 gtatcgatcg cacgccacac tggttaatta agcaggcgat tttttcttat ctcgaacaac
    13861 tggaaaacag cgatactctg ccagagctac ctgcgctgct ttctggcgct gccaatgaga
    13921 gcgatgaagc accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc
    13981 aaattttgcc ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgt cgcccggaaa
    14041 ccgaagcggt ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg
    14101 cgcacaaact ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggtc
    14161 gcgcaggtat ggtccagggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg
    14221 cgctgatgtg tctggcggaa gcgttgttgc gtattcccga caaagccacc cgcgacgcgt
    14281 taattcgcga caaaatcagc aacggtaact ggcagtcaca cattggtcgt agcccgtccc
    14341 tgtttgttaa tgccgctacc tgggggctgc tgtttactgg caaactggtt tccacccata
    14401 acgaagccag cctctcccgc tcgctgaacc gcattatcgg taaaagcggt gaaccgctga
    14461 tccgcaaagg tgtggatata gcgatgcgcc tgatgggcga gcagttcgtc actggcgaaa
    14521 ccatcgcgga agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt
    14581 acgatatgct gggcgaagcc gcgctgaccg ccgcagatgc acaggcgtat atggtttcct
    14641 atcagcaggc gattcacgcc attggtaaag catctaacgg tcgtggcatc tatgaaggtc
    14701 cgggcatttc aatcaagctg tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc
    14761 gggtaatgga agagctttac ccacgtctga aatcactcac cctgctggcg cgtcagtatg
    14821 atattggtat taacatcgat gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc
    14881 tggaaaaact ctgtttcgag ccggaactgg caggctggaa cggcatcggt tttgttattc
    14941 aggcttatca aaaacgctgc ccgttggtga ttgattacct gattgatctc gccacccgta
    15001 gccgtcgccg tctgatgatt cgtctggtga aaggcgcgta ctgggatagt gaaattaagc
    15061 gtgcgcagat ggacggcctt gaaggttatc cggtttatac ccgcaaggtg tataccgacg
    15121 tttcttatct cgcctgtgcg aaaaaactgc tggcggtgcc gaatctaatc tacccgcagt
    15181 tcgcgacgca caacgcccat acgctggcag cgatttatca actggcaggt cagaactact
    15241 atccgggtca gtacgagttc cagtgcctgc atggtatggg cgagccactg tacgagcagg
    15301 tcaccgggaa agttgccgac ggtaaactta accgaccgtg tcgtatttat gccccggttg
    15361 gcacacatga aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca
    15421 cctcgtttgt taaccgtatt gccgacacct ctttgccact ggatgaactg gtcgccgatc
    15481 cggtcactgc tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga
    15541 aaattcccct gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc
    15601 tcgctaacga acaccgtctg gcctcgctct cctctgccct gctcaatagt gcactgcaaa
    15661 aatggcaggc cttgccaatg ctggaacaac cggtagcggc aggtgagatg tcgcccgtta
    15721 ttaaccctgc ggaaccgaaa gatattgtgg gctatgtgcg tgaagccacg ccgcgtgaag
    15781 tagaacaggc gctggaaagt gcggttaata acgcgccaat ctggtttgcc actcctccgg
    15841 ctgaacgcgc ggcgattttg catcgcgctg ccgtgctaat ggaaagccag atgcagcaac
    15901 tgattggtat tctggtgcgt gaagccggaa aaaccttcag taacgccatt gccgaagtgc
    15961 gcgaagcggt cgattttctc cactactacg ccggacaggt gcgggatgat ttcgctaacg
    16021 aaacccaccg tccattaggg cctgtggtgt gtatcagccc gtggaatttc ccgctggcga
    16081 ttttcaccgg gcagatcgct gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg
    16141 cagaacaaac gccgctgatt gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac
    16201 cccctggcgt ggtgcaattg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg
    16261 gtgatgatcg cgtgcgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc
    16321 agcgcaatat cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa
    16381 ccggcggcat gaacgcgatg attgtcgatt cttcagcact gaccgaacaa gtcgtcgtgg
    16441 atgtgctggc ctcggcgttc gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt
    16501 gcctgcaaga tgagattgcc gaccacacgt tgaaaatgct gcgcggtgca atggccgaat
    16561 gccggatggg taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag
    16621 cgaaagccaa tattgagcgc catattcaga ccatgcgtag caaaggccgt ccggtgttcc
    16681 aggcggtgcg ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc
    16741 cgacgctgat cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc
    16801 tgcatgtggt gcgttacaac cgtaaccagc taccagagct gatcgagcag attaacgctt
    16861 ccggctatgg tctgacgctt ggtgtccata cgcgtattga tgaaaccatc gcccaggtca
    16921 ctggctcggc ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggtgg
    16981 gtgtgcagcc attcggcggc gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc
    17041 tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc
    17101 gtcaggatgc agagtatccg gtcgatgcac agttgaaagc cgcattgact cagccgctaa
    17161 atgcactgcg ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg
    17221 gcgagctggc gcaggcagga acacaacgat tgctgccggg gccgacgggt gaacgcaaca
    17281 cctggacgct gctgccgcgt gagcgcgtgt tgtgtattgc cgatgatgag caggatgcgc
    17341 tgactcagct cgccgccgtg ctggcggtgg gcagccaggt actgtggccg gatgacgcgc
    17401 tgcatcgtca gctagtaaag gcattgccat cggcagtcag cgaacgtatt caactggcga
    17461 aagcggaaaa tataaccgct caaccctttg atgcggtgat cttccacggt gattcggatc
    17521 agcttcgcgc attgtgtgaa gccgttgctg cgcgggatgg cgcaattgtt tcggtgcagg
    17581 gttttgcccg tggcgaaagc aatatccttc tggaacggct gtatatcgaa cgttcgctga
    17641 gtgtgaatac cgctgccgct ggcggtaacg ccagtttaat gacgatcggt taaacaggta
    17701 gccggaggat gttacaacct cctccggcat ctttaacgtg gtcgaatccc ctcaataata
    17761 atccgctgca cgttttcaac cgtttgattg aaaaatatct catcgcgcaa cgtcgcgcct
    17821 gtcaccgcct ccacctgagg ggcgaaatcg gcgtaatgtt gagtggaagc ccaaatcatg
    17881 aaaatcaaat gctgcggatc aatcggcgcg agtttgccgc ttttgaccca accggcaatc
    17941 agcgccgatt tctcatcaat taatgccttc aaatcgcccg ttagttcatc cattaacagc
    18001 ggcgcgcctg ccagcatctc catacagaac aggcgcgaag cctgcggata atcgcgggag
    18061 acttccagct tcagacggat gtactctttt atcgccgcca gcggagcgaa atcttcacga
    18121 aacgctttta acggtgccag ccagatatcg agaatctgcc gcagcacggc aatatacagc
    18181 gcctcttttg acggaaagta atacagcaga ttggtttttg aaacacccgc caactctgcg
    18241 atctgctcca gccttgtgcc gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta
    18301 agaatcgctt ttttcttcgc gcttactgcg cgcgaacgtt tacccgttgt tttcactgcg
    18361 ccttgcgtca tgcgctctcc cctcttgtag attggcaaca ggatagcaaa acccccgccc
    18421 ggcctcgacc ttctgcactc tcatcgcgca cagatgcatg ttttatgtgc aactgttttg
    18481 accgtttagt ccacttttta ccagatattt tagataacgc attgttaaca tattaacaac
    18541 attaactttt taaaaactgg catccgcttt gcaaacaagc cattacgcgg ctgaaaagga
    18601 tatgcaggat gcagcgcccc gtctgacttt cactttacga gacgaagaga ggttgatgat
    18661 gaaaattggc gtattcgtac ctattggcaa caacggctgg ctcatttcga cccacgcgcc
    18721 gcagtacatg ccgacctttg aactgaataa agccattgtg caaaaagcgg agcactacca
    18781 tttcgatttc gccctgtcga tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg
    18841 ggatcacaac cttgagtcgt tcaccttgat ggcggggctg gcggccgtca cctcgcgcat
    18901 tcagatttac gccactgctg ccaccttaac gttacctccg gcaatcgtcg cccgtatggc
    18961 cgcaaccatc gactccatct ctggcgggcg ttttggcgtc aacctcgtga ctggctggca
    19021 aaagcccgag tatgagcaga tgggtatctg gcctggcgat gactatttct cccgtcgtta
    19081 cgactatctc accgaatatg ttcaggtgct gcgcgacctg tggggcacgg ggaaaagcga
    19141 ttttaaaggc gattttttca ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtccc
    19201 catgaaagtg atctgcgccg ggcaaagcga cgctggcatg gcgttctccg cccagtatgc
    19261 cgatttcaac ttctgtttcg gcaaaggcgt aaatacgccc acggctttcg ccccgaccgc
    19321 tgcgcggatg aaacaggccg cagagcaaac cgggcgcgac gttggttctt atgtgttgtt
    19381 tatggtgatt gccgatgaaa ccgacgatgc cgctcgcgcc aaatgggaac actacaaagc
    19441 gggcgcggat gaagaggcgt taagctggct aaccgaacaa agtcagaaag atacccgctc
    19501 cggtactgat accaacgtcc gtcagatggc cgatcccact tcggcggtaa acatcaatat
    19561 ggggacgtta gtcggttctt acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt
    19621 gcctggtgcc gaaggcgtgc tgttgacctt cgatgatttt ctgtcgggaa tcgaaacctt
    19681 cggcgagcgc attcaaccac tgatgcagtg ccgcgcccat ctccctgcgc tgactcagga
    19741 ggtggcatga tgacaacctt aaccgctcga ccggaagcca ttaccttcga tccgcagcaa
    19801 agtgcgctga tcgtggtgga tatgcaaaac gcttatgcca cgccaggcgg ctacttagat
    19861 ctcgccgggt ttgatgtctc aaccactcgc ccggtcattg ccaacattca aaccgccgtg
    19921 accgcagcgc gagcggcagg gatgctgatc atctggtttc aaaatggctg ggatgaacag
    19981 tatgtcgaag ctggcggacc cggctcaccg aattttcata aatcgaacgc cctgaaaacc
    20041 atgcgtaagc agccgcagct gcaggggaaa ttgctggcga aaggctcctg ggattatcaa
    20101 ctggtggatg aactggtgcc gcagcctggc gatattgtgc tgccgaagcc gcgctacagc
    20161 ggtttcttca atacaccgct ggacagcatt ttgcgcagcc gtggaatacg ccatctggtt
    20221 ttcaccggta tcgctaccaa cgtctgcgtc gaatcgacgc tacgcgatgg cttttttctg
    20281 gagtatttcg gcgtggtgct ggaagacgca acacaccagg cggggccgga atttgcacag
    20341 aaagccgagt tgttcaatat cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc
    20401 tgcgacgcgc tttctcccac gtcctttgct cgtatcgctt aaggagttta acgatgccaa
    20461 aatccgtaat tattcccgct ggcagtagcg caccgctggc ccccttcgtt cccggcacgc
    20521 tggctgatgg cgtggtgtat gtctccggta cgctggcttt tgatcaacat aataacgtgc
    20581 tgtttgccga tgacccaaag gcgcaaaccc gccacgttct ggaaactatc cgcaaggtga
    20641 tcgagacggc gggtggcacg atggcggatg tgaccttcaa cagcatcttt attaccgact
    20701 ggaaaaatta cgccgcgatt aacgaaatct acgccgagtt ttttccgggt gataaaccgg
    20761 cgcgattctg cattcagtgc ggactggtaa aacctgacgc gctggtggac atcgccacaa
    20821 ttgcgcatat cgccaagtga ggccgcgatg aaactttcac tctcacctcc cccttatgct
    20881 gatgcgcccg tagtggtgtt gatttcgggt cttggcggta gcggcagtta ctggttaccg
    20941 caactggcgg tgctggagca ggagtatcag gtagtctgtt acgaccagcg cggcaccggc
    21001 aataatcccg acacactggc agaagattac agtatcgccc agatggcagc ggaactgcat
    21061 caggcgctgg tagccgcagg gattgagcat tacgcggtgg tcggccatgc gctcggtgcg
    21121 ctggtgggaa tgcagctggc gctggattat cccgcgtcgg taactgtgct gatcagcgtt
    21181 aacggctggc tacgaataaa cgcccatacg cgccgctgtt ttcaggttcg cgaacgatta
    21241 ctgtatagcg gcggcgcgca ggcatgggtg gaagcgcagc cgttgttcct ctatcccgcc
    21301 gactggatgg cggcccgcgc acctcgcctg gaggcagaag acgcgctggc actggcgcat
    21361 tttcagggca aaaataattt actgcgtcga cttaacgccc tcaaacgcgc tgactttagt
    21421 caccatgcgg atcgcatccg ctgcccggtg caaatcatct gcgccagtga tgatctgctg
    21481 gtgccaacag catgttccag tgaacttcat gccgccctgc ccgatagcca gaaaatggtg
    21541 atgccctatg gcggacacgc ctgcaacgtg accgatcccg aaacgtttaa tgctctgtta
    21601 ctcaacgggc ttgccagcct gttacatcac cgtgaagccg ccctgtaagg aattgctatg
    21661 aacgaagccg ttagcccagg tgcgcttagc accttgttca ccgatgcccg cactcacaac
    21721 ggctggcggg agacacccgt cagcgatgag acgttacggg agatttatgc cctgatgaaa
    21781 tgggggccga catcagctaa ctgttctccg gcacggatcg tgtttatccg cacggcagaa
    21841 ggaaaagaac gtctgcgccc ggcactttcc agcggcaatc tgcaaaaaac cctgaccgcg
    21901 cccgtcaccg ctatcgtcgc ctgggacagt gaattttatg aacggttacc gcaactgttt
    21961 cctcacggcg atgcccgcag ttggtttacc tccagcccac aacttgccga agaaacagcg
    22021 tttcgcaaca gttccatgca ggcggcctat ctaatcgtcg cctgccgtgc gctgggactg
    22081 gataccggcc cgatgtcggg ttttgaccgt caacacgttg acgacgcctt ttttgcgggc
    22141 agcacgctga agagcaatct gctgattaat atcggctatg gcgatagcag caaacttttt
    22201 gcgcgcctgc cacgtctgtc ctttgaagaa gcctgcgggc tgttgtaagg agcaaaccat
    22261 gaacattgtc gatcaacaaa cctttcgcga tgcgatgtcc tgtatgggcg cggcagtcaa
    22321 tatcatcacc acggatggtc cagccgggcg cgccgggttc accgccagcg cggtctgcag
    22381 cgtgaccgac acgccgccta cattactggt gtgcctgaat cgtggggcgt ccgtctggcc
    22441 ggtattcaat gaaaatcgaa cgctgtgtgt aaatacactg agcgccgggc aggagccgct
    22501 ttcaaatctt tttggcggca aaacgcccat ggaacaccgc tttgccgccg cccgctggca
    22561 gacgggtgtg accggatgcc cgcaactgga agaggctctg gtttcgtttg actgccgtat
    22621 cagccaggtg gtgagcgtcg gcacccacga cattctgttt tgcgccatcg aagcgattca
    22681 tcgtcacgcc acaccctacg ggctggtgtg gttcgatcgc agttatcacg cgctgatgcg
    22741 ccctgcttgt taacctcctt aaggagacag ctgatggcaa tgttcggttt tcctcactgg
    22801 cagttaaaat cgacctctac agagagcggc gtggtcgcgc cgtatgaacg attgccgttt
    22861 gctcagacgg cagtaatggg cgttcaacac gcggtggcga tgtttggcgc gacggtgtta
    22921 atgccgattc tgatggggct ggatcccaat ctttccattt taatgtcggg gatcggcacg
    22981 ttactgtttt tctttattac cggcgggcgc gtccccagtt atctcggttc cagcgcggct
    23041 tttgtcggcg tggtgattgc cgcgacgggc tttaacggtc agggcattaa cccgaatatc
    23101 agcattgcgc tgggcgggat tatcgcctgt gggctggttt ataccgtcat tggcctggtg
    23161 gtgatgaaaa ttggtacacg ctggattgaa cgactaatgc cgccggtggt gacgggtgcg
    23221 gtggtgatgg cgattggcct gaacttagcg cctatcgcag tgaaaagtgt atcggcttcg
    23281 gcctttgata gctggatggc ggtaatgacg gtgctgtgta ttgggctggt ggcggtgttt
    23341 acccgtggca tgatccagcg gttattgatt ctcgtaggtt taattgtcgc ctgtctgctg
    23401 tatggcgtga tgaccaacgt tttagggctg ggaaaagccg tcgattttac tctcgtcagc
    23461 catgctgcct ggttcggttt gccacatttt tccactcctg cttttaatag tcaggcaatg
    23521 atgctgattg cgcccgtggc ggtgattctg gtggcggaaa acttaggtca tctcaaggcg
    23581 gtcgccggga tggccggacg caatatggac ccgtatatgg ggcgggcatt cgtcggtgat
    23641 gggctggcga cgatgctttc tggttctgtc ggtggcagcg gcgtaaccac ctacgccgag
    23701 aatatcggtg taatggccgt gacaaaagtc tactcgacgc tggtgtttgt cgctgctgcc
    23761 gtcatcgcca tgctgttggg cttttcaccg aaatttggcg cgctgattca taccattccg
    23821 gccgcggtga ttggtggtgc atcaattgtt gtgttcgggc tgattgccgt cgcaggcgca
    23881 aggatatggg tacaaaaccg tgtcgattta agccagaacg gtaatttgat tatggtcgca
    23941 gtaaccttag tgctgggcgc gggtgatttt gctctcacgc tgggcggttt tacgttggga
    24001 gggattggta cagcaacctt tggcgcgatt ttactcaatg cgttgttaag cagaaagttg
    24061 gttgacgttc caccgccaga agttgttcat caggagccat aaacagaaaa ccgccgggat
    24121 taccggcggt gatgactttt tgacgagcct aagaaaaaaa tgcattcgtc attgattagc
    24181 ccggctagtt gtcgcttttg ccgtgactgc tcttgccacc ttttttacct gcttcagatg
    24241 cgcgctgcgg gtcattcttg aaattacccc cgctgtgctg tccacctttt ttacctgctt
    24301 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
    24361 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
    24421 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcgggcaga cccgcgtttg
    24481 gtaatatcct gcaacagctt cttagcgaga atagatatca tctcaaataa ttatccataa
    24541 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
    24601 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
    24661 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
    24721 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
    24781 gatggcacgc gcagtcgctg agggtgcaag caaagtcgat ggcgcagaag ttgtcgttaa
    24841 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
    24901 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
    24961 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
    25021 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
    25081 cggcggtcag gaacaaacta ttacttcaac ctggacgacc cttgcgcatc acggcatggt
    25141 aattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
    25201 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
    25261 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
    25321 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
    25381 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
    25441 gccgggtacg acgaaaaaat ggcggaaaag atttgggaag aaggtagcga tgaagtgtta
    25501 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
    25561 cgtaaaaacg tttaaccagc gactccccca cttctcgcgg gggagttttc tgttatttca
    25621 ccgcctcatt caacacgcta tcaaacttat ccattgggca gaaaccgttg gcatctatcg
    25681 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
    25741 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
    25801 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
    25861 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
    25921 cgtgcccaac taacaccgtg attttcggtg cacgggtgcg atcggtgaca agggctttgt
    25981 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc
    26041 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
    26101 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
    26161 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttcgcgc
    26221 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
    26281 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
    26341 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
    26401 ccgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
    26461 accccgggaa tgcgccggta ataaagaact gtgcggtcgc aacggtacgt tgcagactat
    26521 tggcataggc ataaacggtg tacggcggcg ggcattcccc cgatttcacc atcccctgct
    26581 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
    26641 tgagttgccc accggggacg tcccattctg gccatttatt cggcgtcgac tgctccagca
    26701 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
    26761 gtagctgata gccttccggt acggtttgtg cctgagcgtt tgaagcgagt aaaactatcc
    26821 ctgccacagc tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
    26881 actaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
    26941 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
    27001 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
    27061 ctgcggcgtt accaatacag a
//
LOCUS       DAZFAH010000043        27055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFAH010000043 DAZFAH010000000
VERSION     DAZFAH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.043"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHJWB_001740"
     CDS             complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHJWB_001740"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4313389.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NICSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1632..2303
                     /gene="cecR"
                     /locus_tag="ACHJWB_001741"
     CDS             1632..2303
                     /gene="cecR"
                     /locus_tag="ACHJWB_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4313390.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHJWB_001742"
     CDS             2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHJWB_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4313391.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3294..5030
                     /locus_tag="ACHJWB_001743"
     CDS             3294..5030
                     /locus_tag="ACHJWB_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4313392.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5023..6156
                     /locus_tag="ACHJWB_001744"
     CDS             5023..6156
                     /locus_tag="ACHJWB_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4313393.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6167..7273
                     /locus_tag="ACHJWB_001745"
     CDS             6167..7273
                     /locus_tag="ACHJWB_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4313394.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7235..7645)
                     /locus_tag="ACHJWB_001746"
     CDS             complement(7235..7645)
                     /locus_tag="ACHJWB_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4313395.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7778..8539
                     /locus_tag="ACHJWB_001747"
     CDS             7778..8539
                     /locus_tag="ACHJWB_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4313396.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8536..9777
                     /gene="clsB"
                     /locus_tag="ACHJWB_001748"
     CDS             8536..9777
                     /gene="clsB"
                     /locus_tag="ACHJWB_001748"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4313397.1"
                     /translation="MKCSWCEGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9777..10733
                     /locus_tag="ACHJWB_001749"
     CDS             9777..10733
                     /locus_tag="ACHJWB_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4313398.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10769..11482)
                     /locus_tag="ACHJWB_001750"
     CDS             complement(10769..11482)
                     /locus_tag="ACHJWB_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4313399.1"
                     /translation="MNSRSANNIINDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11552..12256)
                     /locus_tag="ACHJWB_001751"
     CDS             complement(11552..12256)
                     /locus_tag="ACHJWB_001751"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO4313400.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            complement(12401..13105)
                     /locus_tag="ACHJWB_001752"
     CDS             complement(12401..13105)
                     /locus_tag="ACHJWB_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4313401.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13242..13694)
                     /gene="moaE"
                     /locus_tag="ACHJWB_001753"
     CDS             complement(13242..13694)
                     /gene="moaE"
                     /locus_tag="ACHJWB_001753"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4313402.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13696..13941)
                     /gene="moaD"
                     /locus_tag="ACHJWB_001754"
     CDS             complement(13696..13941)
                     /gene="moaD"
                     /locus_tag="ACHJWB_001754"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4313403.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13934..14419)
                     /gene="moaC"
                     /locus_tag="ACHJWB_001755"
     CDS             complement(13934..14419)
                     /gene="moaC"
                     /locus_tag="ACHJWB_001755"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4313404.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14422..14934)
                     /gene="moaB"
                     /locus_tag="ACHJWB_001756"
     CDS             complement(14422..14934)
                     /gene="moaB"
                     /locus_tag="ACHJWB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4313405.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14956..15945)
                     /gene="moaA"
                     /locus_tag="ACHJWB_001757"
     CDS             complement(14956..15945)
                     /gene="moaA"
                     /locus_tag="ACHJWB_001757"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4313406.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15933..16077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16342..17250
                     /gene="yvcK"
                     /locus_tag="ACHJWB_001758"
     CDS             16342..17250
                     /gene="yvcK"
                     /locus_tag="ACHJWB_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4313407.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSIASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(17442..19463)
                     /gene="uvrB"
                     /locus_tag="ACHJWB_001759"
     CDS             complement(17442..19463)
                     /gene="uvrB"
                     /locus_tag="ACHJWB_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4313408.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20042..20719)
                     /gene="bioD"
                     /locus_tag="ACHJWB_001760"
     CDS             complement(20042..20719)
                     /gene="bioD"
                     /locus_tag="ACHJWB_001760"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4313409.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20712..21467)
                     /gene="bioC"
                     /locus_tag="ACHJWB_001761"
     CDS             complement(20712..21467)
                     /gene="bioC"
                     /locus_tag="ACHJWB_001761"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4313410.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHALVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     SRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(21454..22608)
                     /gene="bioF"
                     /locus_tag="ACHJWB_001762"
     CDS             complement(21454..22608)
                     /gene="bioF"
                     /locus_tag="ACHJWB_001762"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4313411.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22605..23645)
                     /gene="bioB"
                     /locus_tag="ACHJWB_001763"
     CDS             complement(22605..23645)
                     /gene="bioB"
                     /locus_tag="ACHJWB_001763"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4313412.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSKRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23732..25021
                     /gene="bioA"
                     /locus_tag="ACHJWB_001764"
     CDS             23732..25021
                     /gene="bioA"
                     /locus_tag="ACHJWB_001764"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4313413.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQK
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25080..25556
                     /locus_tag="ACHJWB_001765"
     CDS             25080..25556
                     /locus_tag="ACHJWB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4313414.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25708..26991
                     /locus_tag="ACHJWB_001766"
     CDS             25708..26991
                     /locus_tag="ACHJWB_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4313415.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVN
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
BASE COUNT         6339 a         7237 c         7075 g         6404 t
ORIGIN      
        1 tcatcagcct gatgccgggc atagcccggc attaaagatt actgcgcagc agcaggttta
       61 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac
      121 ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg gcgtggttgc
      181 tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccgttc
      241 tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg
      301 atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc
      361 agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg gtggacataa
      421 tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga
      481 ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cacccgattt aaaatcagcc
      541 agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact acggatgcca
      601 tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt aaacaccagc
      661 acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt gcgtttctta
      721 tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg
      781 atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc
      841 gcagagaaca gcaggttctg gcgtttcgct ggcagttttg ttaacacgcg acggatatcg
      901 tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga
      961 tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt tgccaccaga
     1021 acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc gccaaacacc
     1081 accagcgaac aaatgttcaa atatttgctg taatcacgga cgttttcgcc aatctgcgcc
     1141 gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc
     1201 ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttaccggta
     1261 ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc
     1321 tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt
     1381 aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt
     1441 tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt
     1501 gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct
     1561 tagccatact ggacaactcc catatggagt cataatctta atcaatcatt tgattaagaa
     1621 tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc
     1681 tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga
     1741 tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag
     1801 atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc
     1861 atgccgagga agccgaacgc ttgttcgccc aaccacagcc tgatcgggct gccatccgtg
     1921 aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca
     1981 acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctat cacctggtgc
     2041 acgaacaggt gattagcccg ctacacagcc acctgacacg cctgattgcc gcctggaccg
     2101 gctgcgacgc caacgatacc cgcatgatcc tccatactca tgcgttgatt ggtgagattc
     2161 tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg
     2221 aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag
     2281 gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggat tggcggtagt
     2341 ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg
     2401 cctgacgctg tatggcaacg tggatattcg tacggtaaat cttagtttcc gtgttggggg
     2461 gcgcgttgaa tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc aggtgctggg
     2521 cgaactggat cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gcgtctcggt
     2581 ggcacaggcg cagtatgatc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc
     2641 cgccgcagcg gtgaaacagg ctcaagccgc ctatgactat gcgcagaact tctataaccg
     2701 ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc
     2761 ctcgcgcgac caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg
     2821 ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc agcctcgaac aggcgcaggc
     2881 gcaactggcg caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg
     2941 cacgctgtta acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt
     3001 taccgtttca ctaacgcgtc cggtgtgggt acgtgcctat gttgatgaac ggaatcttga
     3061 ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta
     3121 tcacggacag attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac
     3181 accggatctg cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga
     3241 tgcgttacgc cagggaatgc cagtgacggt acaattcggt gacgaggcag gacatgaatg
     3301 atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg
     3361 tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggttggtg gggccggacg
     3421 gtgcaggtaa aaccacgctg atgcggatgt tggcgggatt actgaaaccc gacagcggca
     3481 gtgccacggt gattggcttt gatccgatca aaaacgacgg cgcgctgcac gccgtgctcg
     3541 gctatatgcc gcagaaattt ggtctgtatg aagatctgac ggtgatggag aacctcaatc
     3601 tttacgcaga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc
     3661 tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc tccggtggga
     3721 tgaaacaaaa actcggcctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg
     3781 atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg
     3841 agctggcagg cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc
     3901 agtgccgtga cgtgttactg atgaacgaag gcgagttgct gtatcagggg gaaccaaaag
     3961 ccctgacaca aaccatggcc ggacgcagtt ttctgatgac cagtccacac gagggcaacc
     4021 gcaaactatt gcaacgcgcc ttgaaactgc cacaggtcag cgacggcatg attcagggga
     4081 aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg
     4141 ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgca tttattgatt
     4201 tgctgggtgg tgccggaacc tcagaatcgc cgctgggcgc aatattacat acggtagaag
     4261 gcacacccgg cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg
     4321 ccgccaccga tcacgtcaac tttgccgtta aacgtgggga gatttttggt ttgctggggc
     4381 caaacggcgc gggtaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt
     4441 ccggccaggc gctggtgctg gggatggatc tgaaagagag ttctggtaaa gcgcgccagc
     4501 atctcggcta tatggcgcaa aagttttcgc tctacggtaa cctgacggtc gaacagaatt
     4561 tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc
     4621 gtatgagcga ggcgttcggc ctgaaaagta tcgcctccca cgccaccgat gaactgccat
     4681 taggttttaa acagcggctg gcactggcct gctcgctgat gcatgaaccg gacattctgt
     4741 ttctcgacga accgacgtcc ggcgttgatc cgattactcg ccgcgaattc tggctacata
     4801 tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag
     4861 cggaatattg cgaccgcatc ggcctggtgt accgcgggaa attaatcgcc agcggcacgc
     4921 ctgacgattt gaaagcacag tcggctaacg atgagcaacc cgatcccacc atggagcagg
     4981 cctttattca gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt
     5041 tcctggcgtc gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg
     5101 agtagctggc tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc
     5161 attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg
     5221 gcgctggatt tcacccacac tatgaccggt tcgccctaca tcgacgccac catcagcgat
     5281 aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt
     5341 cccgtggatt tcgccgaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc
     5401 accgacggca gtgagccgaa taccgctaac tttgtgcagg ggtatgtcga agggatctgg
     5461 cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat
     5521 gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt
     5581 gcggtgacca tcatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga
     5641 gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg
     5701 ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg
     5761 ctggtgtcag tgtttattct cggcgtgccg tatcgcggtt cgctgctgat tctgtttttt
     5821 atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc
     5881 cgcaaccagt tcaatgccgc tcaggtcgct ctgaacgcgg cttttctgcc gtcgattatg
     5941 ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac
     6001 attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt
     6061 ccggtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc
     6121 ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt
     6181 atggacgtta atccgcaaag agttgcagtc gttgctgcgc gaaccgcaaa cccgcgcgat
     6241 tctgatttta cctgtgctta ttcaggtgat cctgttcccg ttcgccgcca cgctggaagt
     6301 gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac
     6361 ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga
     6421 gatccgccca accatcgaca cacaaaaggc gttactcctg gtgcgtttcc cggctgactt
     6481 ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa
     6541 ctccaacagt gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca
     6601 ggagctgctg gaaggaaaac cgaaacctaa taacagcgag ctggtggtac gcaactggta
     6661 taacccgaat ctcgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac
     6721 tattggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct
     6781 cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc
     6841 ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta
     6901 tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga tttatggttt
     6961 atcgctggtg ggattcggtc tgttgatttc atcactctgt tcaacacaac agcaggcgtt
     7021 tatcggcgtg tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt
     7081 ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac
     7141 ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt
     7201 gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg
     7261 taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga
     7321 ttgccagccc tgcaattgcc agcaaaaagt tgttttccag tacacgggac aacagccaca
     7381 gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca
     7441 cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact accgtgacac
     7501 aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca
     7561 ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga ccagttgcga
     7621 cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc
     7681 agagtaccga actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca
     7741 ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt
     7801 ttcgttcaag gtgctcacca tcaacattca caaaggtttt accgcgttta accgacgctt
     7861 cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg tttgcctgca
     7921 ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc
     7981 gcactacgag tttctcgccg acacgatgtg gagcgatttt gcctacggtc gcaatgccgt
     8041 atacccggaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga
     8101 gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt
     8161 gccaccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc
     8221 gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga
     8281 accggtattg gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa
     8341 agtgcaggcc ggactggatg agatttttac ccgcgcccac ggacgcccgg cgcgcacgtt
     8401 tccggtgcaa tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag
     8461 cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag
     8521 tgcggagatt catttatgaa atgtagctgg tgcgaaggca ataagatcca gttgctggaa
     8581 aacggcgagc aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc
     8641 attcttgaaa cgtttatctg gtttgaggat gacgtcggca aacagctgca tgcggcacta
     8701 ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg
     8761 gatctcagcg atgagtttgt caatgaactg acggcagctg gcgtagtgtt ccgctactac
     8821 gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa
     8881 attgtggtga tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat
     8941 atgtccagct acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt
     9001 gtcgaagata ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc
     9061 tggtggcgac gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg
     9121 ctggtctggc gcgataacga agaacatcgc gatgatattg aacgtcacta tctgaaaatg
     9181 ctcactcagg cgcggcgaga agtcattatc gccaacgcct acttcttccc cggctatcga
     9241 tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag
     9301 ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt
     9361 aaaggcggcg ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg
     9421 atggacgatc actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg
     9481 aatctcgaag caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat
     9541 ctgaacggca ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc
     9601 acctggtgga acctgaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg
     9661 ctggttggct ggcttccggc acacacacca cgtctggcgc aggttgatcc ccccgcacaa
     9721 ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga
     9781 gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct cacctggcta ttttttatcg
     9841 cggtgattgt gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg
     9901 tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct
     9961 atctgattta cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga
    10021 agcgccaggt gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct
    10081 gggtcggcgg cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca
    10141 ctattacgcg gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg
    10201 cagggattat ctttaccgca ggcgtggtgg agttgccgga tcactggtat gtcgatcaaa
    10261 ctacgctgcg tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt
    10321 gcgctttcgc gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcat
    10381 ggaaattcgc cctcgctcag atgctgattt ccagcgttaa ctggatggta atgggagcga
    10441 taatctggct gttacttggt caaagcgtga actatttctt tgtactgggc gtgttgctgg
    10501 ttagtagtat tgctggtgtc atcgtgcata ttccggcggg aattggtgtg ctggaagcgg
    10561 tgtttatcgc gctactggct ggggagcata catccaaagg cacaattatc gccgccctac
    10621 tcgcttatcg cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc
    10681 tactggaaag ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaagtttag
    10741 ttaagttctt tagtatctgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga
    10801 caagcgttat agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg
    10861 ttaatgcacc tgtaacagcg caacgaaccg gactccacaa ctcttcacta tggcatttcc
    10921 ccgccaattc aaggagctta tttcgtccat gcgagataat gcctgaccat aacacaatca
    10981 ttaagcaact tattacccaa acgggtcgtt cactcattaa tgcggcattc atgatgatca
    11041 ccagggccaa ccctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat
    11101 taaataataa accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga
    11161 cgataatggc aggtacagga gctaaactag caacgacaat cgccaccaaa gagagtaaaa
    11221 atagatatat tacgcctgcg atgacggcat ttatcttgtt gatatcctta cttaagccat
    11281 acatacaaag taataataat cctatggcga catacatttg cggtgaatca tattttgttg
    11341 ccgtaatttc gagatacttc gctacccagg ccagtcctgc ggttgtcacc aaacctaaag
    11401 cggtaatgag ccatatgctg tttaatatcc tggcttcgtg ctgaaaatca ttaattatgt
    11461 tatttgctga gcgcgaattc attattttta tatccttaag ttaaatatca ttatctattc
    11521 caattaacgc aataaaactc gtcaattcgc actaccacac taaaccatta ataaattcaa
    11581 gaactgttac agtaatccta aagaggacgc ttaaaatact aaaatagagt gttattgccc
    11641 caggaacgat acagcgaaga gaacctgaaa tttcagaggt gtcggcaaca cggatcaaat
    11701 cttccagtgt tgttgcttca caggcagccg taatgcccca gataaagaca gtcaaaatac
    11761 tcgctaacca cacggaaaaa ctgctggtaa ggatcaggtt aacaacaaaa gcaatactca
    11821 aaccacagaa aatatatgaa tataactgtc tgactggtgg gataacccta ttactacaag
    11881 agcaaatcag cgccattgcg gcaaacatcc ctcccgtaat tcccattacc gtcgctatgc
    11941 tcgaaaaggt ataaaggctg gcgaagagaa agccaacaag aaggattatt agcagataga
    12001 ccagacatgc gcctgataca ttcagtcttt tcagggcgtt gtttaaccag agaccaccta
    12061 ttatcagcgc agccaaagcg aagacaaacg cacctggaga cttttgtgac gtaacccaat
    12121 gcaaatgctg aaatatccag gcacagcctg ccgtagccca tactccagct gctgttaacc
    12181 accaaatgag aagcagcatt ttgctgagcg aagcactttt ttgctgtaat tgagtagcag
    12241 tggattgtct gttcaaatgc gttatctgtc cctgaaatta cgtagtggac atagtctggc
    12301 atttgccgcg attcagttct catgtaaatg agcggaatag tctttataaa atccgaaaaa
    12361 aatcccggct ccaggccggg atcagcagga agaagagaat ttaacggcgg ttgccgaaga
    12421 tccgcaacaa catcaggaac aggttgatga agtccagata caaggttaac gcgccaagaa
    12481 tggaatattt gcgcaggttc gacgtgtcgc gggtatcaat ctgctcaccc atatttttca
    12541 gtttctgcgt gtcatacgcc gtcaatccga caaagacaat cacgccgatg taggtaactg
    12601 cccacatcaa tgcttcgctt ttcaaccaga agttgaccag cgatgccagc acaatgccga
    12661 ttaacgccat aaacagcata ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt
    12721 acaggctcat tgcgccgaac atcccggcag taacgacgaa agtactggcg atagaagcag
    12781 cggtatagac aatgaatata ctggaaagcg taagacccgt cagcgccgaa taaagcataa
    12841 agagcatcgt cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca
    12901 atgctaattg cgcgatgatc agaccgatta aaaagacacg gttagtgaac aacagctcca
    12961 tcacggccgc ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg
    13021 tcatccagcc atagacttgc gccatataag tttgcaagcc agcccggggt tgtacgattg
    13081 aatcagaacg tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg
    13141 atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa acgtatgtac tgagcggtga
    13201 aattgccgga cgcagcggtg ccttatccgg ctaacaaaaa actaccagcg ttttgccgcc
    13261 tgctgatcgc tctcccgagc ttcaacccag cggtcgcctt ccggcgtggc ttcgcgcttc
    13321 cagaacggtg cgcgggtttt gagataatcc ataataaact gcccggcttc aaacgcactg
    13381 ctgcgatgcg cactggtgac accgacaaaa acgatttcat cgcccggcca taattccccg
    13441 atgcggtgaa tcacagtgac gcgccccagc ggccagcggt tacgcgcttc atcaacaatt
    13501 tctgccagtg ctttttcagt catccccgga tagtgttcga gggttaatgc tttgacgctg
    13561 tcgcccaggt tatggttgcg caccttacca gtaaaggtga ctaccgcacc gtcttcgtca
    13621 cgctccgcca gccacgggta ctcttctcct acgctgaacg gctgcggacc aacaacaatt
    13681 ttggtttctg ccatcttaac ctccggttac cggcgggaag aaagctactt cgtcgccgtc
    13741 agtcagcgga tggtcaaaac tcaccagcgt ctggttgacg gcagccagta atttgccatc
    13801 ttccagcgcc agcgcccagc gatcgctctg cgcagccatg tgctggcgta acgcttcaac
    13861 agttgggaaa tccgcagcca cttcggttgc atctgttccc accaactcgc gcacctgggc
    13921 gaaaaaaaga actttaatca tccgcttcca ccttaaagtc acccgacttg ccgccgctct
    13981 tcgccagcaa acgtaccgga ccaatcacca tatctttttg caccgctttg cacatgtcat
    14041 aaatggtcag cgccgccacg gaggccgcgg ttaatgcttc catttcgaca ccggttttcc
    14101 cggtcaggcg gcataaggtt tctatacgca cccgattgtg ctccggctcg gcctgtaaat
    14161 tgacttcaac tttgctgagc atcagcggat gacagagcgg gatcagatcc caggtgcgtt
    14221 ttgccgcctg aataccggca atacgcgcag tggcaaatac gtcgcctttg tggtggcgac
    14281 catcaataat catcgccagc gtctcgctgc gcatggtgac aaaggcttcc gcccgcgctt
    14341 cacgcacggt ttccgctttg gcggagacat ccaccatgtg cgcttcgcca gcggcgttga
    14401 tatgggtcag ttgcgacata cttatttctt caaatgtgga tggaaattac acggacgcgt
    14461 acgggcatcc agctgcggcg cgatgatatt ttcccatgcg gtacggcacg ctttggtcga
    14521 acccggcatg gcgaaaatca gcgttttgtt ggcgacgccc gctaccgcac gagattgcaa
    14581 cgtggaagtg ccaatctctt caaaagacaa catccggaac acttcaccaa aaccttcaac
    14641 ttcacggtcg aacaacggca gcaatgcttc gggagcctga tcaccttccg tcaggccagt
    14701 accacccgta atcaatacca cttgtacatc gtcgctggcg atccacgcag atacctgagc
    14761 gcgaatagcg tagcggtttt ctttcacaat ggctttatcg acaacgtgat ggcccgcttc
    14821 ttgcgccgaa tcgcgcagat agtgaccgga ggtatcgtct tcttcaccgc gacgattaga
    14881 aaccgtaaga atagcaatac gggtcgggat aaattcagtg cttacctgac tcatctgatc
    14941 tctccttttg acgttttagc cgccaatgta cgataagttt tgcgtaatac cggtgttgtt
    15001 ttgatgcagg aaatgggtct gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag
    15061 cgcctgttgc tgggtatcgt cttccagcag atcgcgcagg ttaacgccgc cttcaccaaa
    15121 caggcagaga tggagtttac caatggagga aacgcgcagg cggttgcaag tggcgcagaa
    15181 gtctttttca tacggcatga taaggccaat ctctccggcg taatctggat ggcaaaagac
    15241 ttgcgcggga ccgtcgctgc gttgacgtaa ttggtggatc cagccgcgac gcagtagctc
    15301 gtcacgcaga acctgaccag agatgtgatg cttacggaag agctcgctgc cctcgcccgt
    15361 ttccatcagt tcgatgaaac gcagctggat agggcgatgc tggatccagt tcagaaaggt
    15421 gtcgagctgg tgatgattaa catcacgcat cagcacggta ttgactttga ccttctcaaa
    15481 accggcctca aatgcagcat caatccccgc catgacctgg ttgaatttat cctgcccggt
    15541 aatagcgtga aactggcggg cgtccagact gtcgacacta acgttaatgc cagtaagtcc
    15601 cgcatcgcgc cagttcgcca catcgcgttc cagacggtaa ccattggtgg tgaccgcaat
    15661 ctggcggata gcgtcgtttt cccgcacagc ggcgatgata tcggtaaagt cgcggcgtaa
    15721 agacggctca ccgcctgtca ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt
    15781 aacccggcga atttcatcga cggtaagaaa gcctttattg gtgacgccgc tcggtttgta
    15841 gccatccggc aggcagtagg tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa
    15901 gtagtaaaac ttacgcgcaa atgcatcagt cagttgtgaa gccatgtaca cctttccaga
    15961 tacgggaggc gaagtcattt cttccttcgc cctggtggca atctttacga gcattgtcac
    16021 ggccaaagca ccgtatcagt tgacccaggt gcagaggcta gagtgtttag tggttatgcc
    16081 gatactagcg tgtaaatgag atttttacca tccacacttt cactatgtaa tcatgtatat
    16141 agcgtcatga tcgtgctatt tcgctacaga ataagggaaa atggcggttt tactttgata
    16201 tacatcatca gggaggcata gcgtgcatcg tcctatagag gtagaagaaa atagtttgta
    16261 tcctcaatat tggcaggtta attgctgttt ccccgcaatt tgcgctactg tatcgcggat
    16321 aatctgactc caggaaacta tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt
    16381 gctctcggcg gagggcatgg actgggacgc gttctctcat cactttcgtc tttgggttct
    16441 cgtttaacgg gtatcgtcac caccacggat aatggtggct cgacggggcg tattcgccgt
    16501 tcagaaggcg gcattgcctg gggcgatatg cgcaactgcc tcaaccaact gataacggaa
    16561 ccgagcatcg cctccgcgat gtttgaatac cgttttggtg gcaatggcga actttccggt
    16621 cataatctcg gaaacttgat gttaaaggcg ctggatcacc ttagcgtgcg gcctctggaa
    16681 gccatcaatt taattcgtaa tctgctgaaa gtggatacgc atttgattcc aatgtcagag
    16741 catcccgtcg atctgatggc gattgacgat caggggcatg aagtttacgg cgaggtcaat
    16801 atcgaccagt taactacgcc gattcaagag ttattattaa cgccgaatgt acccgcaacg
    16861 cgtgaggcgg ttcacgctat caatgaagcg gatctcatca ttattgggcc tggcagtttt
    16921 tataccagcc tgatgccaat tctgctgctg aaggaaatcg cccaggcatt acgccgcacg
    16981 ccagcgccga tggtctatat cggcaatctg gggcgtgagt tgagtttacc tgcggctaat
    17041 ttgaagctgg aaagcaagct ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat
    17101 gcggtcatcg tcgggccaaa agtggatgtc tcggcggtga aagagcggat tgtgattcag
    17161 gaggtactgg aggccagcga tattccgtat cgtcatgacc gccagttgtt acataacgcg
    17221 ctggaaaagg cgttacaggc tttaggttaa cggaaatccg aagaaaaatt ccggcttcct
    17281 attgaagaca aagtgcgcgt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
    17341 caaaccgcgc taattcaatg tattgcagag atcatgtagg cctgataagc gtagcacatc
    17401 aggctgtttt ccgtttgtca tcagtcttct tcgctatcct gttacgatgc ggcaataaac
    17461 aactcacgca actgatgcaa ctggtcacga atttgtgccg cttcttcgaa ctccagattc
    17521 tgcgcgtgtt gcatcatcag cccttccagt tcatggattt tctgctgcaa cgctttcggc
    17581 gacatatcca tcggcacatt atccggctca acaatcgggc gcgattttcc tctgcccttc
    17641 gctttggttt tggcaatgtt ctgccccagc gccaggatat cgaccacttt cttgttcaag
    17701 ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcacg gcgacgttcg
    17761 gtttcgccaa tcgctttcgc cattgatggg gtgatcttat cgccgtagag aatcgcttta
    17821 ccgttaacgt tacgtgccgc acgaccaatg gtctggatca acgaacgttc ggaacgcagg
    17881 aagccttctt tgtcagcgtc gaggatcgcc accagcgaca cttccggcat atccagacct
    17941 tcgcgcagta agttgatccc taccagcacg tcgaactcac ccagacgcaa gtcgcggata
    18001 atctccatac gttcgacggt gtcgatatct gagtgaagat aacgcacgcg ctcgccatgt
    18061 tcttcgagat attcggtaag atcttccgcc atccgcttgg tcagtgtggt gaccagtacg
    18121 cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc
    18181 accggccgca cttcgataat cgggtcaagc aatccggttg gacgcaccac ctgatccacc
    18241 acatcgccgc cggatttttc cagctcgtaa ttacccggcg tcgccgaaac atagatggtt
    18301 tgcggcgcta atgcttcgaa ctcttcaaac ttaagcggac ggttatccag cgctgatggc
    18361 aggcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg
    18421 ccaatttgtg gaatggtgac atgagattca tcgaccacca gcagcccatc ggcagggagg
    18481 taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag
    18541 ttttcaatcc ccgaacagta gcccagctcg ttcatcatct ccagatcaaa ctgggtacgc
    18601 tgggtcagcc gctgctcttc cagcagtttg ttgttttcca atagcacttt gcgtctggcg
    18661 gccagctctt ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag
    18721 tgcgttttcg ggtagatggt aaaacgtgga atggtggaaa caatctgccc ggttagcggg
    18781 tcaaataacg acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca
    18841 tccgattctg ccgggaagat atcaatcacc tcgccacgaa cgcggaaagt accacgctgg
    18901 aatgcttgat cattacgagc gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga
    18961 tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc
    19021 agaccataaa tcgcggaaac agaggccact acaaccacat cacgccgctc cagcatcgct
    19081 ttggtggcgg ataaacgcat ctgctcaata tgttcgttaa ccgaggcatc tttctcaatg
    19141 aaagtgtcgg aactcggtac ataggcttcc ggctgatagt agtcgtagta ggagacgaaa
    19201 tactccaccg cgttttccgg gaagaactct ttcatttcgc catacagctg ggccgccagc
    19261 gttttgttgg gcgcaagtac catggttggg cgctgaaggt cagcaatgac attggcaatg
    19321 gtgaatgttt tcccggagcc agtcacgcca agtaacgtct ggtgcgccag gccatcttcc
    19381 agcccctctt cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa
    19441 ttcaatttga acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt
    19501 aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa aacagttcat
    19561 caccataata tttctgttac agcgtaaact ccgctgtcaa tcatgagcaa gatttactct
    19621 gtggcgagat aaaactccgg ccttaccggg ttatccccaa agcaacggct tttttaacat
    19681 ttgtcaagat gagtgatgac agttttatgg caagagatgc ctgttcagtg actgccattg
    19741 caattatcta accaattaaa aaataaaaga tatttctttg agccgtcttt aacgccaatt
    19801 cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt ctaactctaa tacacatagt
    19861 tatccacagg aatagtggat aactgtctgc agcccctatc ccccgccgcc tgggcaattc
    19921 ccacattcac cgcatcatgt ggaggtaaaa tttttttcgt gattatttta gaatttaatt
    19981 ggttaaattg cagtcaatcg aagactcgat ctcgctcgca atttaaccaa atacaggatg
    20041 gctacaacaa ggcaaggttt atgtactttc cggttgccgc attttctgga ttttctgcaa
    20101 gccaggggat ctctcccagc agcggcgcgg gaatcatgcg ggtgagcgtg gtcatatatt
    20161 cagcgtgacg ttttcccgga ggcgtaacat cgttcgccac ccaacccgcc agagtcagtc
    20221 cggcgtgttg tattgcctgc gcagtcaaca tcgcgtgatt aatacagccg agtttcacac
    20281 caactaccag tatcaccggc agttgttcct gtgttaccca atctgcaaaa gtgaaagtgt
    20341 cagaaagcgg cgtaaaccag ccgccagcac cttccactaa cacccagtca gcctgttgtt
    20401 caagcgcgcg taatccggcg ctcattacca atgattctat cggcctgccc tcttgcgcgc
    20461 tgatgatgtg cggcgaggtg ggttctgcga aggtgtaagg atttaccgtt gcgtaatcca
    20521 gctgcaggct gctgttgtgc tgtaacgcca gcgcgtcact attacgtaag ccttccgggg
    20581 tcttttcgct gccagaggcg accggtttat aacctgccgt ccgataacct actgccttcg
    20641 cggcttgtaa aagtgcacaa ctggcgacgg ttttccccac ttcggtatcc gttccggtga
    20701 caaaataacg tttactcacg agcaatcact cccaaaaaaa gatgatacgt cagaggatat
    20761 cgcccctgtt gttgcggcca ggccagttgc aatcgctgca attgcgaacg cgataatatt
    20821 cgcgggtcgc gcccttcatg aagatgcgta gcaccgatgc ctttcagcga acgcatggca
    20881 ctgagcgcat catcaaacca tagtgtgatg ggctgaatat gatgttgata atgcacgccg
    20941 tttagcgact gttcgatttc atctggcggt aaaaagcgat tagcatgcgg acgctcgtcc
    21001 accgcctgcc acgcctgatg cagttcgggt aacgatccct gcaccagcgt ggtaaacgcc
    21061 accacgcctc cggggcgcac cacccgatac agctcgcgga gtgccgtgga taaattaccg
    21121 caccactgca ctgcgagatt gctccatgca agatcgaacg tcgcagtcgc taacggcagg
    21181 gattcgatat ctcccgccag ataatggtct gcagcatctt tctggcgggc ttgcacaagc
    21241 attggcggtg agagatctaa cgccgtcacc agtgcgtgac gctctcgcca gtggcggctc
    21301 atccagcctg gcccacaccc cgcgtccagt acgtgggtgt atttacgctg tgggagcatt
    21361 gccagtaaga cgtcagcact ccggcgctgt aactctgcgt gttgctcata gtgcgcggct
    21421 gcccgaccaa atgccgctgc aatggcttgt ttattaaccg ttgccatgca gcacctccag
    21481 cagacggtcg atatcctgca tttcatgcgc agcggttagc gttaagcgca gtcgcgcagt
    21541 accagcgggt acggttggcg ggcgaatcgc cgtgacccag cagccttgct ggcgcagttt
    21601 ttctgccagt tgtaacgcac ggctgttatc accgacgatc aatggctgga tggcgctgca
    21661 tgaatcagcc agcgtaaacg gcaaatcctg tactccggca cgaaaacgcg caatgagtga
    21721 caccagtttt tcgcgccgtg catccccctc atcacggcga atgaccgcca gcgacgcacg
    21781 taatgcctgc gcctgagcgg gcggcatact ggtgctgtag ataaggtggc gggcgaattg
    21841 cagcagataa tccgccaccg tactggagca aagcaccgca gccccactga cgccaaatcc
    21901 tttgccaaaa gtcaccacca gcaattctgg ttttaccttt tgcagccagc agctgccgcg
    21961 cccctgctcc ccgataacgc ccgtgccgtg ggcatcatcg accatcaacc agccattgtg
    22021 ctgttgcgtt acctgctgga tttccgccag tggcgcaata tcgccgtcca tgctgaacac
    22081 gccttctgtc actaccagtt gctgccccgg acagggggaa gcaagcagtc gcgccagatg
    22141 agtgacatcg ttatgagtaa aacggcgaag ctgcgacggg cttaaactgg cagcctccag
    22201 caatgaggca tggctaagcc ggtcagcgac aatgcggtcc tcttttgcca tcatcgcggc
    22261 aatgaccgcc tgattagcgg caaaaccaga gataaatagc agtgcccgcg aatagcccag
    22321 ccactcggcc agttcttctt ccagcgcctg atgcgcaacg ctataaccgc tgacgtgacc
    22381 ggaaccgccg ctaccgacgc caaattgctc cgcaccctgc tgccaggcac ggataatttg
    22441 cggatgatgg cttaaaccta aataatcgtt actggaaaag ttcagatact ggcgatcatc
    22501 cgccaccagc cagcgtccgg ctccttgcgc cactggataa cggcgacgca gggcatcggc
    22561 agcacgccgc gcatcgagcg ccgcgttgat tttctcctgc cagctcatag tgctgccgcg
    22621 ttgtaatatt cgtcagtgtc cggggtcatc agtgcctgct ccagacgctg ctgttgttcg
    22681 ttatcccctg ccagcacggc agtttgctgc ggatttagcc ccagtttgcg aaacagttgc
    22741 aggtctttat cttcttccgg attcggcgtg gttagcagtt tgcaaccgta gaaaatcgag
    22801 tttgcgcctg ccataaagca catcgcctga gtctgttcgt tcatctgctc gcgtccggca
    22861 gaaaggcgca cgtaagaggt tggcatcatg atccgcgcga ccgcaatggt gcgaataaaa
    22921 tcaaaggcat cgacatcatc gttatcggca agcggcgtgc ctttcacctt caccagcatg
    22981 ttgattggta cgctttccgg cggcgtcggc aggtttgcca gttgcagcaa taatccggcg
    23041 cgatctttta ccgtttcgcc taagcccaca atgccgccag aacagacttt gatcccggca
    23101 tcgcgcactt tttccagcgt atcgaggcgt tcctgataag tgcgggtggt gatgatattg
    23161 ccgtaaaact ccggcgaggt gtccaggttg tggttgtagt aatccagccc ggcgttcgcg
    23221 aggcgctgcg cctgagattc actcaacgtg cccagcgtca tacacgcctc cagccccatc
    23281 gcttttaccc cctgcaccat ttgttctagg tacggcatat cgcgttcgtg gggattcttc
    23341 cacgccgcgc ccatacagaa gcgcttcgat cctgccgctt tcgctttgcg cgccgactcc
    23401 agcacctgtt caacttccat caaccgctcg gcttccagcc cggttttgta gcgcgaactt
    23461 tgcgggcagt atttgcaatc ttccggacaa gctccggttt taatcgacag caacgtgctg
    23521 acctgtacct gacgaggatc gaaatgctga cgatgcacct gctgcgcttc aaacagcaga
    23581 tccagcaacg gtttttcaaa taattctgtg acttgcgaca atgtccagcg tgggcggtga
    23641 gccatggggc ttctccaaaa cgtgtttttt gttgttaatt cggtgtagac ttgtaaacct
    23701 aaatcttttc aatttggttt acaagtcgat tatgacaacg gacgatcttg cctttgacca
    23761 acgccatatc tggcacccat acacatccat gacctccccg ctgccggttt atccggtggt
    23821 gagcgccgaa ggttgcgagc tgattttgtc tgacggcaga cgcctggttg acggtatgtc
    23881 gtcctggtgg gcggcgatcc acggctacaa tcacccgcag cttaatgcgg cgatgaagtc
    23941 gcaaattgat gccatgtcgc atgtgatgtt tggcggtatc acccatgcgc cagccattga
    24001 gctgtgccgc aaactggtgg cgatgacgcc gcaaccgctg gagtgcgttt ttctcgcgga
    24061 ctccggttcc gtagcggtgg aagtggcgat gaaaatggcg ttgcagtact ggcaagccaa
    24121 aggcgaagcg cgccagcgtt ttctgacctt ccgcaatggt tatcatggcg atacctttgg
    24181 cgcgatgtcg gtgtgcgatc cggacaactc aatgcacagc ctgtggaaag gctatctgcc
    24241 agaaaacttg tttgctcccg ccccacaaag ccgtatggat ggcgaatggg atgagcgcga
    24301 tatggtgggc tttgcccgcc tgatggcggc gcatcgtcat gaaatcgcgg cggtgatcat
    24361 tgagccgatt gtccagggcg caggcgggat gcgcatgtac catccggaat ggttaaaacg
    24421 aatccgcaaa atatgcgatc gcgaaggtat cttgctgatt gccgacgaga tcgccactgg
    24481 atttggtcgt accgggaaac tgtttgcctg tgaacatgca gaaatcgcgc cggacatttt
    24541 gtgcctcggt aaagccttaa ccggcggcac aatgaccctt tccgccacac tcaccacgcg
    24601 cgaggttgca gaaaccatca gtaacggtga agccggttgc tttatgcacg ggccaacttt
    24661 tatgggcaat ccgctggcct gcgcagcagc aaacgccagc ctggcgattc tcgaatctgg
    24721 cgactggcag aagcaggtgg cagctattga agtacagcta cgcgagcaac tggcacccgc
    24781 ccgtgatgcc gaaatggttg ccgatgtgcg cgtactgggg gccattggcg tggtcgaaac
    24841 cactcatccg gtgaatatgg cggcgctgca aaaattcttt gtcgaacagg gtgtctggat
    24901 ccggcctttt ggcaaactga tttacctgat gccgccctat attattctcc cgcaacagtt
    24961 gcagcgtctg accgcagcgg ttaaccgcgc ggtacaggat gaaacatttt tttgccaata
    25021 acgagaagtc cgcgtgaggg tttctggcta cactttctgc aaacaagaaa ggagggttca
    25081 tgaaactcat cagtaacgat ctgcgcgatg gcgataaatt gccgcatcgt catgtcttta
    25141 acggcatggg ttacgatggc gataatattt caccgcatct ggcgtgggat gatgttcctg
    25201 cgggaacgaa aagttttgtt gtcacctgct acgacccgga tgcgccaacc ggctccggct
    25261 ggtggcactg ggtagttgtt aacttacccg ctgatacccg cgtattaccg caagggtttg
    25321 gctctggtct ggtagcaatg ccagacggcg ttttgcagac gcgtaccgac tttggtaaaa
    25381 ccgggtacga tggcgcagca ccgccgaaag gcgaaactca tcgctacatt tttaccgttc
    25441 acgcgctgga tatagaacgt attgatgtcg atgaaggtgc cagcggcgcg atggtcgggt
    25501 ttaacgttca tttccactct ctggcaagcg cctcgattac tgcgatgttt agttaatcac
    25561 tctgccagat ggcgcaatgc catctggtat cacttaaagg tattaaaaac aactttttgt
    25621 ctttttacct tcccgtttcg ctcaagttag tataaaaaag caggcttcaa cggattcatt
    25681 tttctatttc atagcccgga gcaacctgtg aacacatttt cagtttcccg tctggcgctg
    25741 gcattggctt ttggcgtgac gctgaccgcc tgtagctcaa ccccgcccga tcaacgtcct
    25801 tctgatcaaa ccgcgcctgg tacctcttct cgcccgattc tgtcggcaaa agaagcgcag
    25861 aatttcgatg ctcaacacta ttttgcatcc ctgacaccag gtgctgcagc gtggaatcct
    25921 tccccgatta ccctgcctgc gcaacctgac tttgttgtcg gcccggcggg cactcaaggt
    25981 gtaacgcata ccacgattca ggcggcggta gatgcggcaa ttatcaagcg taccaacaag
    26041 cgccagtata ttgccgtgat gcctggtgag tatcagggaa cggtatatgt ccctgccgct
    26101 ccgggtggaa ttactctgta cggtacaggt gaaaaaccga ttgatgtgaa gattgggctt
    26161 tcccttgatg gtggcatgag ccctgccgac tggcgtcacg acgtcaaccc gcgcggcaaa
    26221 tatatgccag gtaaaccagc gtggtatatg tacgatagct gccagagcaa acgcagcgac
    26281 agtatcggtg ttctctgctc tgcggtcttc tggtcacaaa acaatggcct gcaactgcaa
    26341 aatctgacca tcgaaaacac gctgggcgat agcgtagatg caggtaacca tccggcggtg
    26401 gcactgcgta ctgatggtga ccaggtacag attaacaacg ttaacattct cggtcgtcag
    26461 aacaccttct ttgtcaccaa cagcggtgtg cagaaccgtc tggaaacgaa tcgtcagccg
    26521 cgtacgctgg tgaccaacag ctacattgaa ggggatgtgg atatcgtttc tggtcgcggc
    26581 gcagtggtgt tcgataacac cgaattccgc gtggtgaact cacgtactca gcaagaagcg
    26641 tatgtgtttg caccggctac gctgtccaac atttactacg gtttcctcgc cgtaaacagc
    26701 cgtttcaatg ctttcggtga tggtgtggcg caactgggcc gctcgctgga tgttgatgcc
    26761 aataccaacg gtcaggtggt gatccgtgat agcgccatca acgaaggttt taacacggct
    26821 aaaccgtggg ccgatgcggt gatctctaat cgtccgtttg cgggtaatac cggcagcgta
    26881 aatgataacg acgaaataca gcgcaatctg aatgacacta actacaaccg catgtgggaa
    26941 tacaataacc gcggcgtggg tagtaaagtg gttgcagagg cgaagaagta agagcaatta
    27001 actatttgcc ggatgcggcg taaacgcctt atccggccta cggttcgatg cgatt
//
LOCUS       DAZFAH010000044        26886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFAH010000044 DAZFAH010000000
VERSION     DAZFAH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.044"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..824
                     /gene="artJ"
                     /locus_tag="ACHJWB_001767"
     CDS             93..824
                     /gene="artJ"
                     /locus_tag="ACHJWB_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4313416.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(999..2126)
                     /gene="rlmC"
                     /locus_tag="ACHJWB_001768"
     CDS             complement(999..2126)
                     /gene="rlmC"
                     /locus_tag="ACHJWB_001768"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4313417.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVTHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2167..2655)
                     /locus_tag="ACHJWB_001769"
     CDS             complement(2167..2655)
                     /locus_tag="ACHJWB_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4313418.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2715..3560)
                     /gene="potI"
                     /locus_tag="ACHJWB_001770"
     CDS             complement(2715..3560)
                     /gene="potI"
                     /locus_tag="ACHJWB_001770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4313419.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGTVGIVGFIAWYLMARAEKQRIRDIQ
                     RVRRG"
     gene            complement(3557..4510)
                     /gene="potH"
                     /locus_tag="ACHJWB_001771"
     CDS             complement(3557..4510)
                     /gene="potH"
                     /locus_tag="ACHJWB_001771"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4313420.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4520..5653)
                     /gene="potG"
                     /locus_tag="ACHJWB_001772"
     CDS             complement(4520..5653)
                     /gene="potG"
                     /locus_tag="ACHJWB_001772"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4313421.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKESQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5748..6860)
                     /gene="potF"
                     /locus_tag="ACHJWB_001773"
     CDS             complement(5748..6860)
                     /gene="potF"
                     /locus_tag="ACHJWB_001773"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4313422.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7211..7687)
                     /locus_tag="ACHJWB_001774"
     CDS             complement(7211..7687)
                     /locus_tag="ACHJWB_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4313423.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7775..8677)
                     /gene="rimK"
                     /locus_tag="ACHJWB_001775"
     CDS             complement(7775..8677)
                     /gene="rimK"
                     /locus_tag="ACHJWB_001775"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4313424.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8738..9460)
                     /gene="nfsA"
                     /locus_tag="ACHJWB_001776"
     CDS             complement(8738..9460)
                     /gene="nfsA"
                     /locus_tag="ACHJWB_001776"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4313425.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9444..9731)
                     /locus_tag="ACHJWB_001777"
     CDS             complement(9444..9731)
                     /locus_tag="ACHJWB_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4313426.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9891..10148
                     /gene="grxA"
                     /locus_tag="ACHJWB_001778"
     CDS             9891..10148
                     /gene="grxA"
                     /locus_tag="ACHJWB_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4313427.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10178..10555)
                     /locus_tag="ACHJWB_001779"
     CDS             complement(10178..10555)
                     /locus_tag="ACHJWB_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4313428.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGYPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10825..12510
                     /locus_tag="ACHJWB_001780"
     CDS             10825..12510
                     /locus_tag="ACHJWB_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4313429.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12685..13221)
                     /gene="rcdA"
                     /locus_tag="ACHJWB_001781"
     CDS             complement(12685..13221)
                     /gene="rcdA"
                     /locus_tag="ACHJWB_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4313430.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13305..14513
                     /locus_tag="ACHJWB_001782"
     CDS             13305..14513
                     /locus_tag="ACHJWB_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313431.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14513..15328
                     /gene="ybjI"
                     /locus_tag="ACHJWB_001783"
     CDS             14513..15328
                     /gene="ybjI"
                     /locus_tag="ACHJWB_001783"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4313432.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15414..15698
                     /gene="ybjH"
                     /locus_tag="ACHJWB_001784"
     CDS             15414..15698
                     /gene="ybjH"
                     /locus_tag="ACHJWB_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4313433.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15739..16971)
                     /gene="mdfA"
                     /locus_tag="ACHJWB_001785"
     CDS             complement(15739..16971)
                     /gene="mdfA"
                     /locus_tag="ACHJWB_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO4313434.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GTAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17256..17852
                     /gene="ybjG"
                     /locus_tag="ACHJWB_001786"
     CDS             17256..17852
                     /gene="ybjG"
                     /locus_tag="ACHJWB_001786"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4313435.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17910..18668
                     /gene="deoR"
                     /locus_tag="ACHJWB_001787"
     CDS             17910..18668
                     /gene="deoR"
                     /locus_tag="ACHJWB_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4313436.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18715..19917)
                     /gene="dacC"
                     /locus_tag="ACHJWB_001788"
     CDS             complement(18715..19917)
                     /gene="dacC"
                     /locus_tag="ACHJWB_001788"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4313437.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20164..20790
                     /gene="gstB"
                     /locus_tag="ACHJWB_001789"
     CDS             20164..20790
                     /gene="gstB"
                     /locus_tag="ACHJWB_001789"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4313438.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20787..21902)
                     /gene="yliI"
                     /locus_tag="ACHJWB_001790"
     CDS             complement(20787..21902)
                     /gene="yliI"
                     /locus_tag="ACHJWB_001790"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4313439.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22013..22396)
                     /gene="bssR"
                     /locus_tag="ACHJWB_001791"
     CDS             complement(22013..22396)
                     /gene="bssR"
                     /locus_tag="ACHJWB_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4313440.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22609..23934
                     /gene="rimO"
                     /locus_tag="ACHJWB_001792"
     CDS             22609..23934
                     /gene="rimO"
                     /locus_tag="ACHJWB_001792"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4313441.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23981..25309)
                     /locus_tag="ACHJWB_001793"
     CDS             complement(23981..25309)
                     /locus_tag="ACHJWB_001793"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4313442.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25317..>26886)
                     /locus_tag="ACHJWB_001794"
     CDS             complement(25317..>26886)
                     /locus_tag="ACHJWB_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO4313443.1"
                     /translation="AVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFM
                     TTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIE
                     RSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIV
                     RKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIH
                     KSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRH
                     AMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELG
                     YQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSL
                     CVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQ
                     FTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPM
                     PFSAWDKSGKLVKE"
BASE COUNT         6848 a         6971 c         6458 g         6609 t
ORIGIN      
        1 tatttattgc atataaattc acttgatggc attgttatcc catgccgcag acacggccaa
       61 aaatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt gccgctttac
      121 ttgcttcctt tactttcggt gcttctgccg cagagaaaat caattttggc gtttcagcca
      181 cctatccccc ctttgaatct ataggtgcta ataatgagat tgtcggcttt gatatcgatc
      241 tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac ttttactaat cacgcgttcg
      301 acagcctgat cccgtcactg aaattcagaa aatatgacgc cgtaatctcc ggtatggata
      361 tcacgcccga gcgtagcaag caggtatcgt ttaccacgcc ctactatgaa aactcagccg
      421 tcgtgattgc caaaaaagat acctacaaaa cgtttgccga tctgaaaggc aaacgtattg
      481 ggatggaaaa cggcaccacg caccagaaat atattcagga tcagcacccc gaagtgaaaa
      541 ctgtctccta tgacagttat cagaatgcct ttatcgatct gaaaaatggt cgtattgatg
      601 gggtatttgg tgacacagcg gtggtaaacg aatggctgaa aaccaatccg caactgggcg
      661 ttgctactga aaaagtgacc gatccgcaat acttcggcac cggtctgggc atcgctgtac
      721 gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca attaaagctg
      781 acggtacata tcagaaaatc aatgaccagt ggttcccgca gtaattgaga ctgatgacaa
      841 aagtaagatt gcctgatgcg ctgcgcttat ctggcccaca tcaactctgc aatgtattga
      901 tttagcatga ttttgtaggc cggataaggc gtacacgccg catccggcat gaacaaagcg
      961 cacctgatca gcaatctgaa gccgcacctg cggctttttt attgcttcac cagcagcgtc
     1021 atcacttcat agtgcgcggt gtgcgggaac atatcaaaaa gctgtacccg ttcgatgcgg
     1081 taacctggca gttcgcggat atctttcgcc atggtttggg cgttacagct ggagtagata
     1141 ataaaacgcg gtgccatcgt tgagagataa tcacacagcg gtttaccaat gccgcggcgc
     1201 ggcgggttaa ccagcaccag ctccggcaca tccccctgag cggtggcaaa ctgagtggag
     1261 tccagcgcct gaaattgcaa acgcgttaag cccagttcag cggctgactg ctttgcacag
     1321 gcaatggcct ctgatgcaat ttcgatcccg gttaactgca tgtcaggcgt cgcgcagtgt
     1381 aaaccaaagc cacccacacc gcagaacaga tcccacatat gtgtaactgg cagctgccgt
     1441 acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg ccggattagt ctggaagaaa
     1501 ctttgcggac ggatccacag cggtacgtca ttaaaacgct ctgccagcgc ctgttgttca
     1561 gtcaggtaga tctccgtctc cccttccata atcgccatat gtaccggctg aatattgacg
     1621 gtaataactt tcagttgcgg tagttgttcc tgtaaccacg gcagcgcctt acgcagttgt
     1681 gccagtttgg tttcagaacg cagtacaaag cgcagcatca tgccgccatc gctctggctt
     1741 tcagtcagca gaatgtattt cagttcgcca cgcttacgcg ccacgttgta gggcgttaac
     1801 cccgcacggg cgataaacgg ttttagcgcc gcaaaaacgg gcgcaaaaga ggcaggataa
     1861 agcgggcagt cacaaaggtc ttccggcgtg ccatcgcgat gcagcatacc gagcagtggt
     1921 ttttcaacgc taccactcac caccattttg gctttattac gaaacccttg ttccgggcct
     1981 gacaccggcg cgcaccattc ctcaaccgga aaatcggcga gcagattttt aagatcggcg
     2041 gttttagcgg agagttgctc tggaatcggc tgcgttatcc actgacagga acgacagcga
     2101 cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac atcatcaggg gcgacgatta
     2161 tacacattat tgcaactgga agaaccgccg actggtcgag ggaacgaaca gcagcatcag
     2221 gatgagcata tccggcagct tctgcagcat caggctatgg aagatttcac gttttgattc
     2281 accgggaatg ctgaacagct ccggataacc ataccccagc gaagccgccc acaagtaact
     2341 tgcggcggtg atttgtgtca gcagatatag ccagcgcgcc cagcgacgcc ctttcaccag
     2401 tgaaaacgca caccagatct caatgaaaac cagcaccaga ctgcttaaaa agaccagcgt
     2461 taaactccag gtttgtacgc tgcgatgaat gaactcgcca ataccgcgca cgcccagcgt
     2521 attgaaaatc atcagcacgt cgaggccacg gatcataata atggcgagcg ccgccacctg
     2581 caccagcgca ggcacattca ggcgagcatg agatgaagat gttttcttaa aaaatcccaa
     2641 cgtttcgtct tccatgaaaa caatgccgcg acatgcgcgg cattatgtag ccaggttggc
     2701 aaattttagt gtcttcagcc acgtcttaca cgctggatat cgcgtatccg ctgtttttct
     2761 gcgcgagcca tcagatacca ggcgataaat ccgacaattc cgaccgtacc gagaattaac
     2821 gttgccaggg cgttgatttc cggattcacc cccatccgca cgctggaaaa gaccagcatc
     2881 ggtaacgtgg tggctcccgg cccggaaaca aagctggcga tcaccagatc atcaagcgac
     2941 aaagtaaaag ccagtaacca gccagaaatg atcgcgggca tgatcatcgg tagcgtaatg
     3001 acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg ctgcttcttc tatcgagcga
     3061 tccagttccc gcagacgcga cgaaataacg actgccacat aagccgtaca gaaagtgaca
     3121 tgcgccagcc agatggtaag cataccgcgg tccgcaggcc agccaatggc atgagcaagc
     3181 gcgacgaata ataacaacag cgacaagccc gtgatgacat ctggcatcac cagcggcgcg
     3241 gtgatcataa aggcaaaacc atttgatccg cgaaacctgc caaaacgcac cagcaccacc
     3301 gccgcaatag tcccgaggat cgccgccgcc gttgccgcac aggcagcaat tgtcaggctt
     3361 aaaccaaccg cactcatcat cgcgtcatcg cgcaataact caccatacca gcgcgttgac
     3421 cagccggccc acaccgtcac cagcttcgag ctgttaaacg aatagataac cagcatcagc
     3481 attggcgcgt agagaaaggt aaagcccagc agcaaaatca caatccgcca gggcgaacga
     3541 actaccggta aattattcat ccgtgttctc ccacgctttt ttgctggtgt ttgtggaacc
     3601 acattatcgg cacaattagc agcagcaaca tgatgatcgc taccgccgag gccaccggcc
     3661 agtcgcggtt attgaaaaac tcttgccata gcacgcgccc gatcatgatg ctgtccgggc
     3721 caccgagcag ttccgggatc acaaactcgc ccacagccgg gataaacacc agcatcgatc
     3781 cggcaataat cccaccttta gtcagcggca cgatcacagt aaagaacgtt ttcagcggtc
     3841 gtgcaccgag atccagcgct gcttccacca gcgaataatc aatacgaatc aacgcggtat
     3901 aaatcggcag taccataaac ggcacgtagg cgtaaacaat gccaatataa actgccagat
     3961 tggtatgaag aatggtcagc ggttgatcga taacccccag ccacagcaga aaattattca
     4021 gcacaccgtt gttttttaat attcccatcc aggcataaac gcggatcaga aacgaggtcc
     4081 acgacggcag gatcaccagt agtaataaaa tattacgggt cgaaggctta ctgtgcgcca
     4141 ccgcccacgc cagcggatag ccgatcagta aacagcaaaa tgtcgaaatc gcagccacct
     4201 gtagcgactg gagataagca tcgaaataga gcggatcgtc ggtcagttgc agaaaattac
     4261 cgagattaag agtgatggac agttgcccgt cagcccactc catcagttcg gtataaggtg
     4321 gaatagcgcg cgccatctcc gccaggctta ttttaaagac aatcagaaat ggcagcagaa
     4381 acagcaagat caaccagata tatggcaacg caatgaccag tttgcgccca tgcttcattt
     4441 gcagctgcga caaccacagc ttaaatccgc ccggcggttt cgactgggca gcaggttcaa
     4501 gtgtattcat ctttgctcct taaaccgtca gcaccacaca gctgtccact tcccagcaca
     4561 aacgcacttc gtcgccccag gtcggtaacc ctttacgatg acgatgggcg ttttgtagct
     4621 gggcgctaat catctgccca cttttcagac gaacgtgata caccgaaaga tcgccgagat
     4681 aggcaatgtg tatcacctcc cccaccgcga agttacaacc attggcgggc ggctcttcgc
     4741 aaagcatgat tttttccggg cgcagcgcta cgtgtaccgg cacgttatcg accactgaag
     4801 catccgcatc gactttcagt ggatgcacca gccccggcga atcaagcacc aggccatctt
     4861 cctgactctc tttgagtaca ccttcaaaga cattaaccga accgataaat tcagcgctgt
     4921 agcgggtagt tggatgctcg tagatctctt ccggctcgcc aatctggaca aatttcccgc
     4981 gattcataat ggcgatacgc cccgccatgg tcatcgcctc ttcctgatcg tgggtgacca
     5041 tcacacaagt cacaccgacg cgctccagaa tatccaccac ttcaagctgc atccggtcac
     5101 gcagcttttt atccagcgcg cccatcggct catcgagcag taatagtttc gggcgtttgg
     5161 caaggcttcg ggccagggcc acacgctgtc gctgaccacc ggaaagctga tgaggtttgc
     5221 gtttggcgaa ctcctgcatg tgcaccaggc cgagcatctc atggacccga ctggcaattt
     5281 ccgctttcgg tagtttgtcc tgtttcaggc caaaagcgat gttctgttcc acggtcatat
     5341 gcggaaacag cgcgtaagac tgaaacatca tattgatggg gcgcaggtaa ggcggcacct
     5401 gtgacaaatc gacgccatca agcattatct gtccggcaga aggttgttcg aaacctgcca
     5461 gcatacgcag cagcgtggac ttgccacagc cggatgcgcc cagcagcgcg aagatttcac
     5521 ctttgtagat ggtcaggctg acatcatcca ccgcatgttg accatcgtag gatttggtga
     5581 ggttgcggat ttctaatagc ggcgtcagcg ccttacgggt tttcgcctgc gggcgaggga
     5641 tagcgtcatt cacggctgct ctccggcatt gaatgtgtgc aagcgcacca tgatggtgcg
     5701 aattgccggc gggtgtggtg cgcccgcccg gcatctaagg ctgcggatta ttttccgctc
     5761 ttcactttgg tccacgcgcg ggtgcgcaca cggtcgattt tcgggtcctg cactttcaga
     5821 gtgaacagct tcgcacgaac atccgcaggc ggataaatac ccgggttctc acggacttcc
     5881 gcactcacca gcggcgtggc tgctttattg gcgttggcat agaacacatg gtcggaaata
     5941 tgcgctacta catccgggcg cagcaggtaa ttcaggaact gataggcttc gtctttgttt
     6001 ttggcatccg caggcatggc gaatacatca aagaacgcca tcgccccttc ttttggaatc
     6061 gagaacgaga cattcactcc attcttcgct tccttcgcgc ggtttgacgc ctgccagaca
     6121 tcacctgccc agccgatagc gacgcaaata tcgccgtttg ccaggtcgtt aatgtattga
     6181 gatgaatgga aataacgaat gttcgggcgc agctttaaca gcagatcggt tgccggtccg
     6241 gtgtaatcat ccgctttagt gctgttggga tctttgccga gataattcaa cacggtagca
     6301 aaaacttctt ctggcgcatc caggaaagag acaccgcagc ttttcagttt ttccagattt
     6361 tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt tttcgcccag cacagcttta
     6421 actttatcaa cgttatagcc aatcccggtc gtcgcccaca tatagggcat agcaaattta
     6481 ttgtcgggat cgtgtttggc gaccagcttc agcagctccg ggtcgagatt cttccactcc
     6541 ggcaatttgc ttttgtccag cggctggaaa actcccgcag tcaactggcg ttcaagaaag
     6601 ctggcagatg gaaccaccaa gtcaaagccg gtgctacccg ccattaattt gccttccagt
     6661 acttcgttag agtcgaaaac atcgtagacg actttaatac cggtttcttt ttcaaaattg
     6721 gccaccgtgt ccggggcgat ataatcagac cagttataaa cgtggagtgt tttttgttca
     6781 gccgcgagcg tgccgacaga gacggccatc agagcacccg cagccagacc cgaaagccat
     6841 tttttattta aggcggtcat atctctttcc ttctgaaagt tcgttaacaa acgaacaaaa
     6901 gttgtcacac taaaagatat gcaataaacg tgcatatttt ttatcggcgg ggtgtaagaa
     6961 aagagaactc tctcccggcc agcactgctc gctatataaa agcatcgcaa gaataacttt
     7021 agccagggtt tgagactata gctcggatta aaaaacggta tttaacaaat ttttatgcgt
     7081 atttgctcta tgtgataaag ccaaattgac acaagagaag tgaaataatc tttaagaaaa
     7141 ggttaaaaac agcagataat tttgcagcgc gcagccactg caccagcgac tgcgtgctcg
     7201 aaaggagtta tcagtggagg aaatagtttt cggtaacgtt gttttgccct tcatcgtcag
     7261 tcggtaacag cagttgatgg gcattagctt caagaatgac catcgaaatc tgctcttcgc
     7321 tctgacgcac gaaccatgca aactgctcat aggttacgcc ctgcataacg gataaagact
     7381 ggcaaaccac cagctttggc agattatcat cctgcatatc aagaaatgct ttcacggtca
     7441 gcgaactggc attgatggca gataaatccg ccgccagcgg caatacagct gaaggtcgga
     7501 cttccgccat ggcagaaaac aggatcgtgt tatcgatcag atcgattttg gcatcaaaga
     7561 caccgtcgaa attctgcata tggggcagat gcagagcctg acagttatca cattcaaaaa
     7621 aactcatccc caggtcatcg agccattgac gcagcgtatc cagaccagga acgaccagcg
     7681 atgtcataat tttgtacaac ctctttcgat aaaaagaccg gcacagctta cgcaaaaagc
     7741 gcaggcaaaa ccatgatcag taatgtgatt gcgactaacc acccgttttc aggcaatatt
     7801 cggtcgtagc gtggcgttcg atccagcgga tcattttacc cgcgatgtcg ataccggtgg
     7861 ttttttctat tccttccagc cccggcgacg cattcacctc catcaccaac ggcccgcgat
     7921 tagcacgcag aatatccaca ccagcaacgt ccagcgccat cgttcgcgcg gcttttatcg
     7981 cgatttcacg ctcctgtggt gtgatactgg cgacgcttgc cgcgccgcca cgatgcaaat
     8041 tggaacgaaa atcgccctct ttcgcccgcc gttcaatcgc agcgaccact tcatcgccaa
     8101 caaccagaca gcggatatcg cacccttgcg cctctttgat atattcctgc accagaatat
     8161 gcgcgttcag accgcggaaa gcgtcaatca cgctttccgc cgcctgacgc gtctccgcca
     8221 gcacgacgcc aattccctgc gtgccttcaa ccaacttgac caccagcggc gcaccaccga
     8281 ccatgtcgat taaatcgctg gtatcatccg gcgaatgcgc aatgcccgtg acaggcaggt
     8341 cgatgccctg acgcgccagc agttgcatgg aacgcaattt gtcacgcgcc cgggcaatgg
     8401 cgaccgactc attgagcgga tagctcccca gcatctcgaa ctggcgcagt gccgccgtcc
     8461 cataaaaggt aatggcggtg ccaatacgcg ggatcactgc gtcaaaatgg ggtaacttgc
     8521 ggcctttgta gtgaatagaa gacgccgcag gatttatgtt catgtagcaa gaaagcggat
     8581 caagaatttc aaccaggtga ccgcgctgta tagcggcttc acgcagccgc ttacacgaat
     8641 agagcgttcc atcccgggac aatatggcaa ttttcaccct gcacctctct gtcagacctg
     8701 gtcaaaagcc cgcgtatcat acatcgacgt ggcggtttta gcgcgtcgcc caaccctgtt
     8761 tgtgcaaata atccagaata aatgggcggc tttctttaat gattgttcgg cggatatgat
     8821 cgctccaggt atcccggcga ttattgctgc cacgggtgag gtaatattcc gccagttgct
     8881 cgtcatactg cgccagtgcg cctttatcca gcggttgata gctgttttca tgcaccaaaa
     8941 tggaggccgg taaacgcggc ttaagatccg gattatccgc aggccagcca aggcacagcc
     9001 caaacagcgg cagaacatgc tgcggtaatt taagcagttt cgtcaccgct tcaatattgt
     9061 tgcgcaggcc gccgatatat accccgccca atcccagcga ttccgctgcg attaatgcat
     9121 tctgcgccat cattgccgta tcaacgacac cgagcaacag ttgttccgcc aggccgagct
     9181 gagcatccgg acagatctgt aaatggcggt taaagtcggc acagaacacc cagaactccg
     9241 ccgcttgcgc tacgtgtttt tgcccgccgg tcagcgtcac cagttcttca cgtaacgctt
     9301 tgtcggtaat gcgaataatg ctactgcact gcaaaaaact ggaactggac gtcgcacggg
     9361 cgctgttaat aatcgcctca cgctgcgctt cggaaatggg ttcatcagtg aaatggcgaa
     9421 tggagcgatg gccacaaata agttcaatgg ttggcgtcat tatctttttc tctttctgaa
     9481 cgtgaatatt gcggtggacg gttcatcagc tgtggggcaa gacgttttgc cacctgaaga
     9541 ataaccacca ccgcagcggg aagcatgagc aaaacaccga gaaaaatcat cagaatctgc
     9601 acttctggcc gagaaaatgg ctcaggcagc gacagggagt cgcttaccga cagcagcgcc
     9661 accgccagta gcatcattcc gataaattcc agtatcaaca cgcctttagg caatttaccg
     9721 atcgcgcgca tacgcttccc tctgcaaagt gagccttcag tctaaaactt ttcactgtat
     9781 tgtgtttaac agttatagct tttagcaatt aatgcaacag gttaaaccta ctttcagcga
     9841 atacatttta gcgtgatcat tacaggcata aatctatgag gagagaaata atgcaaaccg
     9901 ttatttttgg tcgttcgggt tgcccttact gtgtgcgtgc aaaagatctg gctgagaaat
     9961 tgagcaatga acgcgatgat tttcagtatc agtatgtaga tattcgtgcg gaagggatca
    10021 ctaaagaaga tctacaacaa aaggcaggta aacccgtaga aaccgtgccg cagatttttg
    10081 tcgatcagca acatatcggc ggctataccg attttgctgc atgggtgaaa gaaaatctgg
    10141 acgcctgatc gtctgacaag ccctcgcgtt gagggcttta ctgatttttt ctgtgctgtg
    10201 gtttaaacaa actactgata aataagaaac acagtgcccc cagcgcacac cagaacaccg
    10261 cgcttagtaa ccatgccagc tcttgccaga atgagcgggt cggtgaaaaa aacagccgca
    10321 taataagcat tgaacagggt gccgccagca ttgcgccaaa tagaggtttc aggacttctc
    10381 tacgctgtga aaagaaactg gcaactgctc caggaagaat gaaaaacagc aagccgattt
    10441 caggataccc ggcagcccga aaagcgcctt tcatgtgcgt cgccagaaaa aggcacacca
    10501 caatgaagag gacaaaacag cagattgccc ccgcccaacg ttgtttatgt ttcactcgtt
    10561 cctcctgaca ctgcgtctat cgaacacatt tttcgccagt gtggcgttca gtaagataaa
    10621 gccgcttcgc attccatgct aatataggcc aacgcaattc atatagccgt tgatacctaa
    10681 tgtgattaca ctagtaaaat atattgttac tttactatcg tttaggtgcg ctgaatgaat
    10741 ctgcgccctg aattctggta aaaaacatta tcgtaaatta ccatttcttt caacagctta
    10801 ctagtaaaca agaagttagc ctccgtgaat ataaacgtcg ccgaattgtt aaatgggaat
    10861 tacattctgt tattatttgt ggtcctcgcg cttgggctat gtctcggaaa gttacgactt
    10921 ggttcgatcc aactgggtaa ttccattggc gttttggtcg tatcactgtt attaggccaa
    10981 caacatttca gcattaacac cgacgcgctt aatcttggct ttatgctgtt tattttctgc
    11041 gtcggggtcg aagccggacc gaactttttt tccatttttt ttcgcgatgg gaaaaattac
    11101 ctaatgttag cactggtgat ggttggcagt gcgctggtga tcgccttagg gttaggtaag
    11161 ctgtttggct gggatattgg cctgacggcc ggtatgttag caggctctat gacgtcgaca
    11221 ccggttctgg tcggtgctgg cgatacactg cgtcattccg gcatggaaag caggcagctc
    11281 tcactggcac tggataatct gagcctcggg tatgccttaa cctatttaat cggtctggtg
    11341 agtttgattg ttggtgcgcg ttacttgccg aaattgcagc atcaggactt acagaccagc
    11401 gcccagcaaa tcgcccgcga acgtggcctg gacactgatg ccaaccgtaa ggtttattta
    11461 ccggtgatcc gcgcctatcg cgtcggcccg gagctggtgg cctggaccga cggcaaaaat
    11521 ctgcgtgaac tgggtattta tcgacaaacc ggctgctaca ttgaacgtat tcgacgtaac
    11581 gggattctgg caaatccaga cggtgatgcc gtgctacaaa tgggcgatga aatagcgttg
    11641 gtaggctatc ccgacgccca tgcccgactc gatcccagct tccgtaacgg taaagaagtt
    11701 ttcgatcgtg accttctcga catgcgtatc gtcactgaag aagtggtcgt taaaaaccat
    11761 aacgctgtag gtaaacgtct cgcacaactg aagttgaccg atcacggttg cttccttaac
    11821 cgcgtcattc gtagccagat tgagatgccg atagatgaca acgtcgtgct taacaaaggt
    11881 gacgttttac aagtcagcgg tgatgcccgt cgcgtaaaaa ccatcgccga tcgcatcggc
    11941 tttatctcga ttcacagcca ggtcactgac ctgctggcat tttgcgcctt ctttgttatt
    12001 gggctgatga tcgggatgat caccttccag ttcagcacat tcagtttcgg catggggaac
    12061 gctgccgggt tgttattcgc cggaattatg ctgggcttta tgcgtgctaa ccacccgacc
    12121 ttcggttaca ttccgcaagg tgcattaagc atggtgaaag agttcggctt gatggtgttt
    12181 atggcaggcg ttggtctgag cgccggtagc ggtattaata acggcctggg cgcgattggc
    12241 ggtcagatgt tgattgccgg attaattgtc agtcttgtgc ccgtggttat ctgtttcttg
    12301 ttcggtgctt atgtattgcg aatgaaccgc gcactgttgt tcggcgcaat gatgggcgca
    12361 cgcacctgcg cgccggcaat ggagatcatc agtgatacag ctcgcagtaa catccctgcg
    12421 ctgggctatg cgggcaccta cgcaatcgcc aacgtcctgc tgacgctggc agggacaatc
    12481 atcgtcatgg tatggccagg attaggataa aactgaagtt gccctgaaaa tgaaattttt
    12541 ttgcacaacc gcagaacttt tccgcagagc atcagtctta attagtgcca ctgcttttct
    12601 ttgatgtccc cattttgtgg agcccatcaa ccccgccatt tcggttcaag gttgatgggt
    12661 tttttgttat ctaaaattta tctactaccc tgcaaccctc tcaaccatcc tcaaaatctc
    12721 ctcgcgcgat agcggcttac ggtcggtgac aaaatgcagc gtcatcccct caataaacgc
    12781 atcaagcgcg cgggcggttc cgggttcaaa ccattgttcg agcgtttgct gactgcgctg
    12841 catccagttt tgcattaccg tttttaatag cggttttcgg ctagccagcg cgtagagctg
    12901 gtacatcagc tccatgttat ccggcgttgc aacctgtgag ctgtagatca tatcggtgat
    12961 agcctggcat gcgccttgag catcactaac atcgctaaaa aatgcctgat attgccggga
    13021 cataatctca gtaaaactgc tgaacgcctc cagcaacaac tcatcaattc ctgaaaaata
    13081 gtaggtcatc gatcccaacg gtaccccggc aagggtagca attttgcggt gcgtaacagc
    13141 atgtattccg taaagtttca ccgcctccag cgtggcctgg ataatttttt ctcgccgttg
    13201 cggatcgtta gcgcgacgca tgtcatttcc tctgtctgca attgtgtaca aatgtacaca
    13261 accttgctaa tgttgtcttc cctcttctta ttttgacgtg atctatgacc gtaaattctt
    13321 cacgtaatgc attgaaacgc cgaacctggg cgctgtttat gttcttcttt ttgccaggcc
    13381 tgttaatggc gtcctgggca acccgtacgc ctgctatccg cgatattctc tctgtctcga
    13441 tcgctgaaat gggcggtgtt ctctttggtc tgtcgatcgg ttcgatgagc ggtattctct
    13501 gctcggcgtg gttagtgaaa cgctttggga cacgtaatgt catcctggtc acgatgtcct
    13561 gcgcattgat cgggatgatg atattaagtc tggcactctg gctgacatcg cccctgctct
    13621 ttgccgttgg tctcggcgtc tttggggcaa gttttggttc tgcggaagtg gcgataaacg
    13681 ttgaaggtgc cgccgttgag cgagaaatga ataaaacggt tttgccgatg atgcacggtt
    13741 tttatagcct gggcacgctg gcaggcgctg gtgtcgggat ggcactgacg gcctttggcg
    13801 ttccggcaac ggtgcacatt tcattggcgg cgctggtagg tatcgcgcct atttatatcg
    13861 ccattcaggc aatccctgac ggtacgggca aaaatgctgc cgatggcacc cagcatggcg
    13921 aaaaaggcat tcctttttat cgcgatatcc agttgctgtt gattggtgtt gtagtgctgg
    13981 cgatggcctt tgccgaaggt tctgccaacg actggttacc cttattaatg gttgacggtc
    14041 acggttttag ccctacttcc ggctcgctga tttatgccgg ttttaccctg gggatgaccg
    14101 ttggacgctt caccggcggt tggttcatcg accgttacag tcgcgttgcc gtggttcggg
    14161 ccagtgcact aatgggggcg ttgggtattg ggctgattat ttttgtcgat agcgcctggg
    14221 tcgcaggagt gtctgttgta ctttggggac tgggtgcctc gctgggcttc ccgctgacca
    14281 tttctgccgc cagcgatacc ggccccgatg caccaacccg cgtcagtgtg gtagcaacga
    14341 ccggttatct ggctttcctc gtcgggccgc cgctgctggg ctatctcggc gaacattatg
    14401 gattacgtag tgcaatgctg gttgtactgg cgctggttat tctcgcggct attgtcgcga
    14461 aagccgtcgc caaacccgat accaaaacgc agacggcgat ggagaatagt tgatgagcat
    14521 taaattaatt gcggtagaca tggatggtac tttcttaagc gatcaaaaaa cctataaccg
    14581 tgagcggttt atggctcagt atcagcaaat gaaagcacaa ggcattcgct ttgtggtcgc
    14641 cagcgggaat caatattatc agttgatctc tttcttccct gaaattgcga atgaaattgc
    14701 ctttgtggct gaaaacggcg gctgggtagt gagtgaaggc aaagatgttt ttaatggcga
    14761 gctgtcgaag gatgccttta ctactgtcgt ggaacatttg ctgacgcgcc cggaagtgga
    14821 aattattgcc tgcggaaaaa atagcgccta tacgctcaaa aaatatgacg atgccatgaa
    14881 aacggtggcg gaaatgtatt atcaccgtct ggaatacgtc gataactttg acaacttaga
    14941 agatattttc tttaagtttg gcctgaatct ttccgatgaa ctgattccac aagtacaaaa
    15001 agcattacat gaggccatcg gcgatattat ggtgccagtc cacaccggca acggcagcat
    15061 cgatctgatt atccccggcg tacataaagc caatggcctt cgccaactgc agaaattatg
    15121 gggaatagac gacagcgaag tggtggtctt tggcgatggc ggtaacgata ttgagatgct
    15181 gcgtcaggca ggctttagtt ttgcaatgga aaatgccggc agcgcggtcg tcgcagcggc
    15241 aaaataccgg gcaggctcca ataaccgtga aggcgtactg gatgtgatcg ataaagtgct
    15301 taaacacgaa gcgccatttg accaataaaa acggcagcac gcccggcaac acaatacctg
    15361 aatgaaggct gcataattcg cggaaattaa cccgctgcat tatttcaggc gaaatgatta
    15421 tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg gcgatggcgc
    15481 aaagtgtcac cgtcgatgtg ccgagtggat acaaagtggt tgtagttcct gattcagtca
    15541 gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg gctcctgcgc
    15601 cagctccggc ttaccgcccg catccttatg ctcgccatct tgccagcgta ggtgaaggga
    15661 tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc tgatcgcaca
    15721 aagcagtcag gcattttttt acccttcgtg agaatttccc atctgtttat cttttaaaaa
    15781 gataaccatc agcgacagcc acaaaattcc gttgacaagg ttgaagagat taaactgtcc
    15841 gttgcccccg ttcagccagg catgtttgct gatttcaata ccaacggtaa agatcagcat
    15901 ttgcagcatt cccatcgccg cagaaaccgt acctttactc atatcgctgg caaacagggt
    15961 taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac ttaacccggc
    16021 agtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga ccaacagacc
    16081 aatcataatc ggccagccgc ccataattat cagcgaacgt acggtgcggc gcgaggtcaa
    16141 acgcgctaac aacaagttac ccgcaattaa cgccccgaaa ataggcactt gcagcaagcc
    16201 atattcatag ctgctcaact gctcgccggt aatgatgata atcggcgact gggcgatcca
    16261 cgccagcaat ggcaggctaa cgaatcccag cgccagcgcc cccgccacaa agcggccgtt
    16321 cttcagcacc agcttatagt cacgaccgag ttctttcagt gacagtttct cgcctatacg
    16381 cgtggcggtt tcaggcatgg ctcgttgcag accgaaaaag gagatcgctg ccaatgcggc
    16441 aaacaaaaca aacatcccct cccagggcag cacatggatc cacgccgtgc ccaccagcgg
    16501 accaagtagc ggagcaatca gcgccacgtt cgccatcagc gcggtgatct tgatacaaac
    16561 cgcctcttcg aaggattcct gaattgcggc gtatcccaca gcgccaatga aacagaggct
    16621 tatgccctgc aagaagcgca acagggtgaa ttgttcaatg ttttgcgcca gcaatattgc
    16681 cagacaggtg atgataaacc acaccacccc cgccagcatc accggacggc gaccaatacg
    16741 atccgacagc ggccccagga gccattgtaa aaacatcccg cccgccagat atgcggtcat
    16801 tgaagtagga acccaatcaa tgcccgcctg atattgttcc accacggcca acataccggg
    16861 ttgaatcata tcgttgccga tataggttga aaattcgtaa agcaccagac agagagggaa
    16921 aagtaacgcc tgacgtccaa gcctggcacc ggaagctaat ttattttgca tgcaatttct
    16981 tcgccaataa taatcgcgca gagtttaata aaagcgcagc taacgagaaa gcgaattttg
    17041 tagctgaaac cacggttaag cacattctta cattattgcg agtatagcta cgctttcttt
    17101 aagttttatt taacctatgc ccgttacaat cacccaccgt aaacaggccg cttgagggaa
    17161 ataagacgat gccgctttac ccagtttaac ctgcacttta ttctcaacga cttgcctgta
    17221 ttggctccct tttaatcact ttgcgtcggg aagttatgct ggaaaatttg aatctctctc
    17281 tattctctct tattaacgcg acgccagact cggctccgtg gatgatctcg ttggcgattt
    17341 ttattgctaa agatttgatt accgtggtac cgctgctggc cgcggtgctt tggttgtggg
    17401 ggcttacagc acaacggcaa ctggtgataa aaatcgctat cgcgctggcg gtcagcctgt
    17461 ttgtgtcctg gacgatggga catctttttc cgcacgaccg accctttgtc gaaaatatcg
    17521 gctataactt cctgcatcat gcggcggatg actcattccc aagcgaccac ggtacggtga
    17581 ttttcacctt tgcactggca tttttatgct ggcatcgcct gtggtccggc tcacttttaa
    17641 tggtgctggc cgtcgtcatt gcctggtcgc gcgtttatct tggcgtccac tggccgctgg
    17701 atatgctcgg tggattgctg gcaggtatga ttggctgcct tagtgcccag attatctggc
    17761 aagcgatggg gcataaactc tatcaacgtc tgcaatcgtg gtatcgcttc tgttttgcat
    17821 taccgatccg caaaggctgg gtgcgtgact gatctctgct aaaaagtgta gtattgagcg
    17881 gctcgcttca ataactattc agagggatta tggaaacacg tcgcgaagag cgtatcgggc
    17941 agctgctgca agaattaaaa cgcagcgata agttacatct taaagacgcc gccgccctgc
    18001 ttggggtttc ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg cccgtcgttt
    18061 tgctcggcgg ctatattgtt ctggaaccgc gcagtgccag ccattacctg ttaagcgatc
    18121 aaaaatcccg cctggtggaa gaaaaacgcc gggcggcaaa actggctgcg acgctggtag
    18181 aacccgatca gaccctcttt tttgactgtg gcaccaccac gccgtggatt attgaagcga
    18241 ttgataatga aatccctttt accgccgttt gttattcgct aaataccttt ctggcgctga
    18301 aagagaaacc ccattgccgc gcgtttcttt gcggtggtga atttcacgcc agcaacgcca
    18361 ttttcaaacc catcgatttt cagcaaacgc tgaataattt ttgcccggat atcgcttttt
    18421 attctgcggc gggcgtgcat gtcagtaaag gcgctacctg ttttaatctt gaagagttgc
    18481 cggtaaaaca ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc gaccacagta
    18541 aatttggcaa ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat attgtggtga
    18601 gcgattgttg cccggaagat gagtatgtga agtacgcgca gacgcagcgc attaagttga
    18661 tgtattaatg acgtataacc ggatgacgtt tcgcgccatc cggttatcag aagattaaga
    18721 gaaccagctg ccgaaccact gatggaattt catcatcacg aaatcccaca tccgaccaaa
    18781 gaatccgccc tcttccacat tttccatcac gatcagcgga cgctgctcaa tggatttacc
    18841 gttaagctgg aaatcaatgg tcccgacaac ctgacctttt ttcagcggtg cggtaagctg
    18901 cggttccgtt aacgtataac tcgctttcag gtttttcagc tgcccacgcg gtatggtcac
    18961 tgagcccgct tcacctgccc cgagattcac ttcgctctta tcaccaaacc agacgcgctg
    19021 agtcacaaag gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc ggaaacccca
    19081 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
    19141 ggagattaaa cgcatatcac cctgggtagc cgaagcaacc agattatatc ccgcacctgc
    19201 cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg
    19261 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
    19321 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
    19381 ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt
    19441 ggcataacca ttcatcagac caataaagga ctcctggctc ccggcaacgt aatcggccag
    19501 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
    19561 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
    19621 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
    19681 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcgggat ccagtttctc
    19741 atccgcgttg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
    19801 cgcatccacg ctcggcgctt caacggtttg ttccgccgcg aatgccgttg gggcaaaaag
    19861 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
    19921 tgccacccga gtatccattc aaaaaaaata cgttatatcg ccgtatgata agttcggtga
    19981 ttaataacgc acacacaaac ttaaagaaac cgtaagttgg taaagttttt aaagtttatg
    20041 caatatcctg ccgcttcgct gcttctggtg tcattttttt gccagctgtt gcttaaaagt
    20101 taactttact tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg aaaaggggtg
    20161 agtatgatta cgctgtgggg tcggaataat tcaactaacg taaaaaaagt attgctgacg
    20221 ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt tgggataaat
    20281 cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt gcgtgacgac
    20341 gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc ggcacagtac
    20401 gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcgga aaaatggatg
    20461 gactgggcaa accagacact cagtaatgct catcgcggga tcctgatggg attagtcaga
    20521 acaccacccg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga gtgcgacgcc
    20581 ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg cgacgagttt
    20641 ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt tggcctgacc
    20701 tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg ccccgcagtg
    20761 cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag taattcccca
    20821 ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac atcacgaatt
    20881 cgctgtcctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc gttgacgctc
    20941 atcacaatga catctttatc tttcagcgcg cctataaata atttttgctg ccactggggg
    21001 aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt ccagtaaaaa
    21061 acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt aaagcctgaa
    21121 tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg cgggatatta
    21181 atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact ccacggattc
    21241 atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg cgcaccggat
    21301 tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg caccagtttg
    21361 ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc cagagcaata
    21421 aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc ggtagacagt
    21481 tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc atcacttaag
    21541 cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga ataacttaac
    21601 cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag gccgccctgc
    21661 ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt tcctgcttgc
    21721 cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc gggtaaaaag
    21781 gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac cgttgcagga
    21841 gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagaa ttgtcgatgc
    21901 ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat aacaaattca
    21961 tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt tttcaggcct
    22021 tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc atcagacaat
    22081 tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc ccactcatca
    22141 aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc cactcttcct
    22201 gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc agtttaaaaa
    22261 agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc ttcctcagtt
    22321 gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga tcgattcgct
    22381 gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat taaatagtat
    22441 ggctactttt tgagcaaaat gctgtccagg atcaattctt cacgccaaat taacaaaatt
    22501 tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat ccagaactct
    22561 gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat gagcaaagta
    22621 actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct tgtcgattca
    22681 gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag ctatgacgat
    22741 gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca agaatcactg
    22801 gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg ttgtctgggg
    22861 gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac cgggcctcat
    22921 agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa acacaaccca
    22981 ttcctgagcc tggtgccaga acaaggtgtg aaactgacac cgcgtcatta tgcctatctg
    23041 aaaatttcag aaggctgtaa tcaccgctgc accttctgca ttattccgtc tatgcgcggc
    23101 gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct ggtagatgcg
    23161 ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt tgatgttaaa
    23221 catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag cctgtgcgaa
    23281 cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta tccgcatgtg
    23341 gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga cattccgttg
    23401 cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt agatcgccaa
    23461 ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg ctcaaccttt
    23521 attgtcggct tccctggcga gacagaagaa gatttccaga tgctactcga cttcctgaaa
    23581 gaagcgcgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg tgcagacgcc
    23641 aatgccctgc ctgaccaggt tccggaagaa gttaaagaag aacgctggaa ccgtttcatg
    23701 cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg tgaaattctg
    23761 gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc agatgcaccg
    23821 gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg ttaagccggg tgatatcctg
    23881 cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt ttaattaatt
    23941 caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag ctaatctgag
    24001 ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc aagcgttcga
    24061 cggtggtaca ttcatttgaa tgcgctacac cgatactagt acttaaatta atgcttccca
    24121 cctggaaatg aacgatttct tttagaatcg atagcaactc ttccagcgtt ccacccgtat
    24181 aaatcaccgc aaattcgtca ccgccgaagc ggtagatttt acctttatct accgggatct
    24241 gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa tgaccgaagg
    24301 tgtcgttaat gtttttgaat ttatcgacgt caaacaggca caaataaccg cctttttccg
    24361 gcagcgcttt taaatcttcg tcaaaggatt ttctgcccat tgctttggtc aggtcgtcca
    24421 ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa aagaaaagcc
    24481 cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc acaataccac
    24541 gattaatcac tctgcgtacc agatagaggc aactggtacc cgatatcact gccaccaatg
    24601 cggcaaagaa aataatcaat gtacagaccc gcaaataaac atctttttta tacgcaaata
    24661 cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg gtgaagatgg
    24721 cgtttttagc attatattca tcaccggtca ccggtttacc gttcttaaat agagagaaca
    24781 tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc tccagaccaa
    24841 gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg aaggttttac
    24901 cgtcaaaaga gataaaccct gtatttttga catgtcgata cttcacaacc tgcataatat
    24961 ctgcacaata atttttctcg aactccttta cctgaataca ttcgtcactg gaaatcagat
    25021 aaggaccgac atcgtacaaa taagaggggg aaaccgagtc ggcgtaagct gtgcgcaaac
    25081 caaccaacag ctgttcctcc gtgggattta gcgtgttgat aagatcaatt gccacgctac
    25141 gccgatattc agcaatattt gccaccgcaa aagcagtatc aataacggac tgtttcaggc
    25201 tatacacccg ttcctttacc atttgagtgt agatcccgca ggcaactgct gaaatcatga
    25261 taaaaatcag cagactgact gtaagtacga acttattgat tctggacata cgtaaactac
    25321 tcttttacta attttccact tttatcccaa gcggagaatg gcatgggttt ctggtacaga
    25381 tacccctgag cctgaacaca tcctaattta attaattcgc gcgcaacgtc ttcgctctct
    25441 acgccttccg caatcaccgg gatccggtta tagcgtgcca gatcgaaaat aatgcgcaca
    25501 atttttcccg cagtaccgaa aatatcgccg gtaaattgcg agtccacctt tatgctgtct
    25561 ggattaattt cataaaaacg tttcaggttt gacaaaccgg tgccaaagtc atcaatcgat
    25621 atccgtaccc cttgtttacg cagttgttca atattgagat aaaaatgttc attaattcgc
    25681 tcgatcacgg tttcagtgat ctcaacacag aggctgttcg ccttcaggcc attttctttc
    25741 atgatggtgg taaaacgctc atgaaagtgc ggttcgttta actgccaggg cgaaacatta
    25801 atatgcagta agaaatcatc cttcagcgac gcacgctgac taaaatgtct gaattcaccc
    25861 attgccgttt taataatctg ataacccagc tcattgataa acccgatatc ttcagcaatg
    25921 gtaatgaact tcagcggtga aatgatcccc cgctccgcag aaacccaacg gcacaacgcc
    25981 tccgcgccca gaatggcgcg ggtgttaata tcaacgatcg gttgatagac gaggaatagt
    26041 tcgccaattt ccattgcatg gcgaatatta cgcgcaatcc agatattctc ttctatttca
    26101 ttgagtttcg cgtcatcaca gaaaataaac tcggagaaca tatggtcacg gatactggaa
    26161 atcgccagtt tcagtttttc tgcgtaactc caggcgtttt ccccctgcaa aggttcacaa
    26221 atggcagact tatggatgcg cagattgcgg tagccaaagc tgctgtcaaa atcgtttacc
    26281 atcgctattt tacgggtaaa tgtctgtaca ttttcccgac acagaagtaa atagagatcg
    26341 gcattgaatg tcacgatatc gcagcattgc gcatatttct cggcaattcg ctgacacaat
    26401 ttcctgacga ttttattgcg ttcatcaaca ccaaacaccg cttccacggc ttccagacca
    26461 cacagtttga tgatccccac agccagatac tgattattgt tggactcaat aatcgccata
    26521 tttttaataa aaccgctttc ctgatcgtaa gagatcagtg agagcaattt atttgaccgt
    26581 tcaatttcgg catctttcat ttccgcgatc gaatttttga tgctgataat ctctttcgca
    26641 tcgccttcgc gaattttcag agcctcgatc tgatccgttg agtcgggcaa aaacgccagc
    26701 gtattaacca gctcggtcat ttcagaaaat tgccgacgga acacacgagt ggtcatgaaa
    26761 tataccacca gcaacacgat aataatgaac gcaaacggta gataaaaaag tttatctgat
    26821 gcgttcaata aattaccgcc aggataataa aaatagattt cgaacaatgc attatttggc
    26881 actgct
//
LOCUS       DAZFAH010000045        26734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFAH010000045 DAZFAH010000000
VERSION     DAZFAH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.045"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..1732
                     /gene="rtcR"
                     /locus_tag="ACHJWB_001795"
     CDS             134..1732
                     /gene="rtcR"
                     /locus_tag="ACHJWB_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4313444.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1714..2472)
                     /locus_tag="ACHJWB_001796"
     CDS             complement(1714..2472)
                     /locus_tag="ACHJWB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4313445.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2489..3319)
                     /gene="glpG"
                     /locus_tag="ACHJWB_001797"
     CDS             complement(2489..3319)
                     /gene="glpG"
                     /locus_tag="ACHJWB_001797"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4313446.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3364..3690)
                     /gene="glpE"
                     /locus_tag="ACHJWB_001798"
     CDS             complement(3364..3690)
                     /gene="glpE"
                     /locus_tag="ACHJWB_001798"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4313447.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3880..5385
                     /gene="glpD"
                     /locus_tag="ACHJWB_001799"
     CDS             3880..5385
                     /gene="glpD"
                     /locus_tag="ACHJWB_001799"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4313448.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5439..6044)
                     /locus_tag="ACHJWB_001800"
     CDS             complement(5439..6044)
                     /locus_tag="ACHJWB_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313449.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISALLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6047..6814)
                     /locus_tag="ACHJWB_001801"
     CDS             complement(6047..6814)
                     /locus_tag="ACHJWB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313450.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYQVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6817..7527)
                     /locus_tag="ACHJWB_001802"
     CDS             complement(6817..7527)
                     /locus_tag="ACHJWB_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313451.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7543..9051)
                     /locus_tag="ACHJWB_001803"
     CDS             complement(7543..9051)
                     /locus_tag="ACHJWB_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4313452.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWPTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9177..11624)
                     /gene="glgP"
                     /locus_tag="ACHJWB_001804"
     CDS             complement(9177..11624)
                     /gene="glgP"
                     /locus_tag="ACHJWB_001804"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4313453.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11643..13076)
                     /gene="glgA"
                     /locus_tag="ACHJWB_001805"
     CDS             complement(11643..13076)
                     /gene="glgA"
                     /locus_tag="ACHJWB_001805"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4313454.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13076..14371)
                     /gene="glgC"
                     /locus_tag="ACHJWB_001806"
     CDS             complement(13076..14371)
                     /gene="glgC"
                     /locus_tag="ACHJWB_001806"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4313455.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14389..16362)
                     /gene="glgX"
                     /locus_tag="ACHJWB_001807"
     CDS             complement(14389..16362)
                     /gene="glgX"
                     /locus_tag="ACHJWB_001807"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4313456.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16359..18545)
                     /gene="glgB"
                     /locus_tag="ACHJWB_001808"
     CDS             complement(16359..18545)
                     /gene="glgB"
                     /locus_tag="ACHJWB_001808"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4313457.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18818..19921)
                     /gene="asd"
                     /locus_tag="ACHJWB_001809"
     CDS             complement(18818..19921)
                     /gene="asd"
                     /locus_tag="ACHJWB_001809"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4313458.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20113..20706
                     /gene="yhgN"
                     /locus_tag="ACHJWB_001810"
     CDS             20113..20706
                     /gene="yhgN"
                     /locus_tag="ACHJWB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4313459.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20763..22103)
                     /gene="gntU"
                     /locus_tag="ACHJWB_001811"
     CDS             complement(20763..22103)
                     /gene="gntU"
                     /locus_tag="ACHJWB_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4313460.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22107..22634)
                     /gene="gntK"
                     /locus_tag="ACHJWB_001812"
     CDS             complement(22107..22634)
                     /gene="gntK"
                     /locus_tag="ACHJWB_001812"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4313461.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLHDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22773..23768)
                     /gene="gntR"
                     /locus_tag="ACHJWB_001813"
     CDS             complement(22773..23768)
                     /gene="gntR"
                     /locus_tag="ACHJWB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4313462.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23992..24687)
                     /gene="yhhW"
                     /locus_tag="ACHJWB_001814"
     CDS             complement(23992..24687)
                     /gene="yhhW"
                     /locus_tag="ACHJWB_001814"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4313463.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24810..25847)
                     /gene="yhhX"
                     /locus_tag="ACHJWB_001815"
     CDS             complement(24810..25847)
                     /gene="yhhX"
                     /locus_tag="ACHJWB_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4313464.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26180..26668
                     /gene="yhhY"
                     /locus_tag="ACHJWB_001816"
     CDS             26180..26668
                     /gene="yhhY"
                     /locus_tag="ACHJWB_001816"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4313465.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6681 a         7149 c         6654 g         6250 t
ORIGIN      
        1 aaaattaaat aagcagctta attttttaat tcattgtttt aaaaaagatt atatctttac
       61 gtccgtaacc ggagatttcc cgcaaagcca atttaccgat aatgaaatat cgtcttttat
      121 aaggatatct aagatgcgta aaacagtggc ttttggcttt gtcggtaccg tactggatta
      181 tgccgggcgc ggcagtcagc gctggtcaaa atggcgtccg acactctgtt tatgccagca
      241 agaatcgttg gtcatcgatc gactggaatt gttgcacgac gcccgctcgc gctcgctatt
      301 tgaaacgctt aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg tgagcgttga
      361 gattgaactg cataacccgt gggatttcga agaggtctac gcctgcctgc atgatttcgc
      421 ccgtggttac gagtttcagc cagaaaaaga agactattta attcacatca ccaccggtac
      481 ccacgtcgcg cagatttgct ggtttctgct ggcagaagcg cgttacctgc ccgcccggct
      541 gatacaatct tcacctccgc gcaaaaaaga acagccgcgc ggcccaggtg aagtaacgat
      601 tatcgatctc gatttaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca
      661 gcaaacgctt gattttctta agtccggcat tgccacgcgt aacccccact tcaaccgcat
      721 gattgagcag atcgaaaaag tggcgatcaa atcccgcgcg ccgattctgc ttaacggtcc
      781 aaccggcgcg ggcaagtcat ttctggcgcg acgcatcttc gagttaaaac aggcgcggca
      841 tcagtttagc ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg ataccgccat
      901 gtcgacgctg tttggtcatg taaaaggcgc gtttaccggg gcgcgggaat ctcgtgaagg
      961 tttattacgc agcgccaacg gcggaatgtt gtttcttgat gagattggcg aactgggcgc
     1021 agacgaacag gcaatgctgc tgaaagccat tgaagagaaa accttttacc cgtttggcag
     1081 cgatcgccaa gtgagcagtg attttcagct tatcgccgga acggtgcgcg atttgcgcca
     1141 gctggttgcc gaaggcaaat ttcgcgaaga tctgtacgcg cggatcaatc tctggacctt
     1201 caccctgccg ggtctacgcc agcgccagga agatattgaa ccgaacctgg attatgaagt
     1261 ggagcgccac gcctcactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc
     1321 ctggttggct tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc
     1381 tgccagcgtc acgcggatgg ccacctttgc cactagcgga cgcatcactc tggacgtggt
     1441 tgaagatgag ataaaccgtc tgcgctataa ctggcaggag agccgcccct ccgcgcttac
     1501 ggcgttgctg ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca
     1561 cgttatcgct atctgccgcc aggcaaagtc gctttccgca gccggacgcc agctttttga
     1621 cgtttcgcgc cagggcaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc
     1681 gcgttttggt ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatggtg
     1741 gtccgtcagc acctgcatca cgcttactgg cggcggggcg tcggtgtaga cggcatctac
     1801 catgctgatg ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac
     1861 cagcataacg tggcgcgagt tctcaataat ggcgcgtttg gtgcgaactt cgtggtaatc
     1921 gaactccagc agcgagccgt cgctatcgat gccgcttatc cccagaatgc cgaaatcaag
     1981 gcggaactgg gagataaaat cgagcgtcgc ttcgccaatg atcccgccat cgcggctgcg
     2041 taattcgcca ccggcgagaa taatgcggaa atcttctttt accatcaacg tgttagcaac
     2101 attgagattg ttggtgacaa tacgcaaatt gctgtgatta agcagcgcgt gcgccaccgc
     2161 ttccggcgtg gtgccgatat cgataaacag tgtcgagcca ttggggattt gctccgccac
     2221 tttgcgggca atcctctctt tttcttcggt ctgggtggct ttacgatcgt gccacggcgt
     2281 gttaaccgaa ctggaaggca gcgccgcacc gccatggtga cgcaggatca ggttttgctc
     2341 cgccagctca ttgaggtcgc ggcgaatagt ctgcgggctg acggagaaat gctctaccag
     2401 ctcttcagta ctgacataac cctgctgttt aaccagttcg ataataccgt tgtgacgttg
     2461 tgtttgtttc atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa
     2521 caaaagccat cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca
     2581 tcccaaacaa atcaaaccat ccggcgacaa tccagatcag cgcaaagata attaacccac
     2641 gttgcaggta aatgccactt tgcggatcgc gttcgccacg tagccagacg tagcccatca
     2701 gcgcatacac cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat
     2761 agccgcttaa cagggcgcta atgagagtaa taacaattag cttaccgcta ccgaggcgtt
     2821 tttccaccgc accgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg
     2881 agaagtgcat taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gttggatcga
     2941 atggccaggc cagccataac atcacttcct gatcgccgag aatttgcatg gcgataaaca
     3001 ccaccacgca ggcgatcatc accacccagg ttaccggacc tgcgcgttca cgcaaggcag
     3061 caaagaaagg ataacggcga taatgcagac cactgccggt atggcccgcc tgccagctgg
     3121 ccgccagata acgcggatct gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc
     3181 gctcggcctg ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtga
     3241 ggataacacc ctgcgtcgcc atataatcaa caaacgcctg cgccacgcgg gggttagcaa
     3301 aagaggtaat catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa
     3361 gcgttacgcg ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaaaccgcc
     3421 gtcaatgcta tagaccacat cgtagccctg ttgcagcaga tactgcgccg cgcctttgct
     3481 gctattgccg tgataacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa
     3541 cgcgcccagc gtgtcgttgg ttaaatggaa agcctgcacc gcatgcccca tagcgaaact
     3601 ctgtggatcg cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc
     3661 ggcaacgtta atacattcga actgatccat gcgtctctct ttctttacaa acaagtgggc
     3721 aaatttaccg cacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca
     3781 ctctaaaatg ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac
     3841 gaacatttat gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt
     3901 gtgataggtg gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta
     3961 tccgtgctga tgctggaggc gcaggatctc gcttgcgcga cctcttccgc cagttcaaaa
     4021 ctcattcacg gtggcctgcg ctaccttgag cactatgaat tccgcctggt cagcgaggcg
     4081 ctggctgaac gtgaagtgct gctgaaaatg gctccgcata tcgccttccc gatgcgtttt
     4141 cgcctgccac atcgtccgca tctgcgcccg gcgtggatga ttcgcattgg tctgtttatg
     4201 tacgatcatc tgggtaaacg caccagcctg ccgggatcaa ctggtttgcg ttttggcgca
     4261 aattcagtgt taaaaccgga aattaagcgc ggattcgaat attctgactg ttgggtagac
     4321 gacgcccgtc tggtactcgc caacgcccag atggtggtgc gtaaaggcgg cgaagtgctt
     4381 actcggactc gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa
     4441 gatatcgata ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa cgccaccggc
     4501 ccgtgggtga aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc
     4561 ctgatcaaag gcagccatat tgtggtgccg cgtgtgcata cccagaagca ggcctacatt
     4621 ctgcaaaacg aagataaacg tattgtgttc gtgatcccgt ggatggacga gttttccatc
     4681 atcggcacca ccgatgtgga atacaaaggt gatccgaaag cagtgaagat tgaagagagt
     4741 gaaatcaatt acctgctgaa agtgtataac acgcacttta aaaagcagtt aagccgtgac
     4801 gatatcgtct ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg
     4861 caggctatta cccgtgatta cacccttgat atccatgatg aaaacggcaa agcaccgctg
     4921 ctgtcggtat tcggcggtaa gttgaccacc taccgcaaac tggcggaaca tgcgctggaa
     4981 aaactgacgc cgtattatca gggcattggc ccggcgtgga cgaaagagag cgtgctgccg
     5041 ggtggtgcca ttgaaggcga ccgcgacgat tatgccgctc gcctgcgccg ccgctatccg
     5101 ttcctgactg aatcgctggc gcgtcattac gctcgcactt acggcagcaa cagcgagctg
     5161 ctgctcggca atgcgggaac ggtaagcgat ctcggggaag atttcggtca tgagttctac
     5221 gaagcggagc tgaaatacct ggtggatcac gaatgggtcc gccgcgccga cgacgccctg
     5281 tggcgtcgca caaaacaagg catgtggcta aatgcggatc aacaatctcg tgtgagtcag
     5341 tggctggtgg agtatacgca gcagaagttg tcgctggcgt cgtaaggatg atgcccggtt
     5401 gtgaagtgca accgggctat ttggattcct tagactattc attgtaaata attgaataac
     5461 ggcccctgag ctaaaatcgc caaacttaag cacgttaccc aatcgcccca cttcttcctc
     5521 tggtttctat tttttctgca ccaatggcaa taatatccca atgcaaaacc aagcagtgca
     5581 ctaatcgggc cgacaaaatc agtccaaggc tgcggccaaa aaggggaaaa attcagggga
     5641 agccagacaa acgtcagaaa gaaatttcgg atattttcca gcaacgctga tttctctgga
     5701 gaaatataaa gaatgaaatt cgagagaatt aaccagaaat agaccggcga aaaaaccagc
     5761 cagcaatgca gtctgttttt taattcagga ggcgttctgc gggagaattg ccaaccgatg
     5821 ccagccatta cactgaaaaa aagtgcatat tcaaccatta actcaagttg tagttcctga
     5881 agagaaatac gcacaaccag gagcggaaag gagaatatta tttctgccag aaaagttaac
     5941 gtgaatataa gtacggctaa gacaataacc gtcggccata ccaataatgg cacgataaca
     6001 aaaccgagaa ttacaggggg aatataaagc cggaatttaa tcatttttat tttcctggtg
     6061 attcggatga tgcgtcatac atcgcgcata aacactcatc aattatccag tcatcttccc
     6121 agacctgata ttctgtcatt ggttcgggct gttttagcgg gttctcaatc gcttgccgcg
     6181 ctctttcttt cagtaaagga tgattatttc tagcccaggc attgatttgg gtggtaatga
     6241 cgttatcgat atcgtcctgg ttatctctga tataatcctc acaatatctg ttcaataaac
     6301 aggcaagaga aacagtcgac caaaagccgg gaactgaatt aatggcatcc agcaatggcg
     6361 caagagcgat atcggcacgg aaaaacattt ccagcatata ctgtaattcg acagcttcat
     6421 cgccggatac ccaatcagtc atatactggc aggcaaatcg ctgtaaaatt gcccgttcat
     6481 ccgcacgcca gttttctttg ttggcaaggt tgatacggcg cagagaaaaa actacgtgaa
     6541 tattgtcaaa atgcccctgt gcaacaaatt ccagtaaacg cggcagaaaa tagcggacct
     6601 catcgctatt ttgcggatca ggccctgggc tactattcca ggcattgatt aaggaatacg
     6661 ggatcgctcg taacgatgta ctccgtaatg ccttttgttc ctgctgactc aggcaatatt
     6721 cacaacaaac cacaaatttc tgcggcacct gataacgacg aaaggtgtga taaatctgct
     6781 caattaatgc ctgcatggcg gggctgggtt gcatagttac cccctcgccc gctgatcgcg
     6841 tgtaaacggt tgatagacaa aacgagcatt cgccgcgttc aggcgaataa gggcgctacg
     6901 cgaaagggaa aagctggggc gaatgcaact attgcagtct cgacagcttt cctctgcgca
     6961 attttccact gagtaatgac gcgtgtagag tttcagttcg gcctgcttat cgcgaaatcc
     7021 gcgtaaactg ttattgttat gaaatttcca ggcatcttta aagacctgaa agatatagac
     7081 atcctcaatc caggagtcac attttttaag cgggcgcttt gctattatca cccgctcatc
     7141 cggatttagc atcgccgtaa attgtacggt gacgtcattt gtcgtgttca ggtcacattg
     7201 aacatctggt acatcactga tataggggtc aaatccaaaa tcagcaccac aggcacaaag
     7261 acggctaatg gtggcggtat cgagggaaaa tcccggccat accttgctgg aggttaagcc
     7321 aatatttaat gtgacatcat atcgaagcaa tccctcaaga ccgtcagcac cgacgaactt
     7381 ttccccttca tccagggcct ggttaataag ttgctgaaca atgccttcat cccagtatgc
     7441 cgatttgcag ttggcaatcg taatatccac gcctgtgaag ggatcagtac cggcaaggct
     7501 tgcaaataat gtattaacta attccatttt tattatccag ctttaaggta gcgtcttata
     7561 catcgggaca taacctacat cttccaccaa actctgtccc tgcggcgtta aaaaccactc
     7621 caacaatttt tgtgtctctg acgtcgtatt ttcccgcgtt accataaagg catcgacgat
     7681 atagggatat ttgccgttgc gaatgttttc ggcagtcggt gcaataccgt taatcgctag
     7741 cagttttata tttttatcgg cgttcatttg tgttgcgtaa taacggaagg tgtaacctat
     7801 tgcgttgttt gtattacggt attcggcaac gactttaatc attccctcca tcatacttgc
     7861 cacttctgtt tcctgcggcg agatcatgcg gacatttttc atgacctggg attgcatcac
     7921 cgtctggcta ccagagtctt ccgggcgttg ccaggtctgg atctcctgat cgttaccgcc
     7981 aacagtaggc cagttggtaa ttgcaccact gaagatgtca cgcacctgtt gttcagtcag
     8041 ggaattaacc ggattatccg cattgacgat gaaaacaaac gcttcacggg caaaaggcgt
     8101 gtacagcaaa gtaacgcccg attcctcagc gcgttttttc tgcccaccgg aaggttgcgc
     8161 cacaaaaata atatcagcat cacctttaac aatcctgtta tacgcatcgg gggtacgaga
     8221 gctctccaga tattccctgg tgtgaaaatc ttccggtata acgcttaaag cataaaatgc
     8281 agaggcataa attgggtacg ccgccgtagc accatcgagt cgcggccagt tttgcgtaaa
     8341 ctgaatttgc ggtttaccac gcagtggtgt cagttgatta ttgagtttat cgggtcgcca
     8401 ggcccaggta tcaatatttt cgcgcacacc gacgccggga ttgagctgtt tatataaata
     8461 accctgataa ccagcgacaa aaccaagcat gagaaggata aacaggaggg aattacggca
     8521 acgtagcccg gatgtaccgg ttaaaggatg tctggttggc cggccgtacc ccagcgaaaa
     8581 actgatatgc gcgccaacgg gtataatgac tatccaccag tactcacctc cgactactga
     8641 gactaacaaa acgcccagcc aggggagcat aagatcagcc aaccaacccg atcccatctg
     8701 gtgaaattga taatctgcca gatgcagcgc gattatccag ccaaccagga ttaataaacc
     8761 gggtactaaa aaaagacatt gtcgccagac ggctatttct cttttttctg gtgcactatc
     8821 gcctgcccat actcgccccg agaaaaatag cattaatgcg cccagcgcca gcataaaaat
     8881 gttgagcaga aggtaacttg ttggcgtgat aaggatatta caaacttcaa taataccggc
     8941 aagtccgaca cccagcatag caacaaccgc cgccaaaaat tgcgtcagta tggggatgcc
     9001 gaaaaaagtc attaccagcg aggacaaaat ccattttctg ttttgcatta ttctttccat
     9061 tctttttgaa tggtgaaatt atactccccg agtccccttg ccccttctgg acacttttcc
     9121 gaaatgatgg cggaaaaaaa cgggaccctt tggccccgtt ctatttattg gtgaacttac
     9181 aatctcaccg gatcgatatg ccagatatga tcggcgtact ctttgatagt acggtcagaa
     9241 gagaagtagc ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc
     9301 tcgtagagtt cgtcgacttt atcctgacaa tcgacatagc tgcgataatc cgccagaacc
     9361 tgatagtgat cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct
     9421 tccggactga atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc
     9481 tcgtagtatt cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgcagtg
     9541 ttaccaaaga taaagatatt gtcagcaccg acatgatcca gcatctcgac gttcgcaccg
     9601 tccagcgtac cgatagtcag cgcgccgtta agcgcaaact tcatgttact ggtgccggaa
     9661 gcttccgtcc ctgccagcga aatctgttca gacagatctg ccgccggaat gatcaactgc
     9721 gccaggctaa cgctgtagtt cgggatgaac acgactttca gcttatcgcc aatctgcgga
     9781 tcgttgttga tcactttcgc tacgtcattg atcaaatgaa taatgtgctt cgccatgtaa
     9841 taggccgaag ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccggg
     9901 tcggccttga tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg
     9961 tattcgtgaa tacgtttgat ttgtacatcg aacaacgcct ttggattcac caccacattc
    10021 agctgctggg cgatatactc tgccagacgc tttttgttct ccagcttcgc ctgatgcaca
    10081 gcgtgattaa ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg
    10141 gtgcgccagt tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc
    10201 gctagccagc gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt
    10261 ttcgcaaagt cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg
    10321 ttaactttgt ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat
    10381 tcatcaatga tcgacgcccg tcccagcaga tcggtatcgt tcggatactg ttcctgcaag
    10441 gttttcagga aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg cagaatttta
    10501 cccagcatat caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag
    10561 gagaagacct gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc
    10621 agcagacgca tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg
    10681 attttatccg ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc
    10741 tgaatggtcg aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag
    10801 gtggagtcat ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact
    10861 gccgcgaagt agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgcgca
    10921 ctccacaaac gcaacgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa
    10981 gcgactccca gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg
    11041 cgaccaccaa aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttaccg
    11101 tattccagcc agtagtctgg cgactctttc tggctaccgt taacgatgtt ctgcttgaac
    11161 ataccgtagt catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa
    11221 tcaaggaagc aagccgccag acgtcccagg ccaccgttac cgaggcctgg gtcattttct
    11281 tcatcaatca gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg
    11341 taaattccta gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac
    11401 aggtagtaaa cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc
    11461 tccacgagac gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg
    11521 acgaccgggt cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct
    11581 tctacgctaa gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa
    11641 aactatttca agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa
    11701 tccattgcca tagcctgacg ttgcacaaac cgccacagtg aaggacggga ccacagtaca
    11761 aaagcacgtc gaatagcccg taacagcgac caggcattac tatcttcaaa gacaaaccca
    11821 ctggcgacgc catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca
    11881 ccggtgcgcc gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg
    11941 cacggttcaa aacggctggg caccagaatg acgtccgcgc cgcccataat gcgatgcgaa
    12001 aatgcttcgt gatagccaat ctgaacgccc acctggccgg ggtattccgc tgccgccgca
    12061 aggaaacctt cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc
    12121 tccagaagac ccggtaaggc ttccagcacc aggtcgagac ctttctggct ggtcagacgg
    12181 ctcaccactg caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac
    12241 tggcgcttat tttccgcttt atcttccaac gtatcgcggg tgtagcgcga ggccaacagt
    12301 aagtccgtct ctggactcca gattttctcg tccacgccgt tcagtacgcc ggaaagacgc
    12361 ccttcacggt gacgctgttg caacagacct tccataccgt aggcaaactg cggttcggtg
    12421 atctcgcgag cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc
    12481 ttcaggaaag agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc
    12541 aattggatgt cattcatatg atgtgcataa aacatgcctt gataggccag gttgtgcaca
    12601 gtaaacaccg acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca
    12661 tgccagtcgt gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt
    12721 tctgccccaa cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag
    12781 ttggtatcgt gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat
    12841 atgccaaccc cgttgtaatg accgaacaac agcgtgatat gtccggcgaa ggtatcacga
    12901 cgggatacta cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt
    12961 acgcgagcgt caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc
    13021 agaccgccgg ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc
    13081 gctcctgttt atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt
    13141 ctgaacgata gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg
    13201 gaataacaca agcacgatcg atgacgcagc ggcgcagacg gcacgagcga cctacccata
    13261 cttccggtaa caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga
    13321 acagaacgga ctgcaccacc accgaaccgg agatcacaca gccgccggat accagtgagt
    13381 taagggtcat cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt
    13441 cattgtaggt gcgaattggc caattgcgat cgtacatatc cagttccggc accacagagg
    13501 ccagatcgag gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct
    13561 cggcatccgg gtcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg
    13621 cttcggtgat cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcacgat
    13681 cgtcttcttc cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg
    13741 ccagagattt gctcggatcg ttcggcattg acggtgggtt agcaggtttt tcaacgaatt
    13801 cgataatttt atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg
    13861 gtactggcat acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac
    13921 gcgagtagtc ttgcttgtag atatggtcgc ccgccaggat caccacgtat tccgctttat
    13981 aacggcggat aatgtcgagg ttttgggtga ccgcatctgc ggtgccgcga taccagtttt
    14041 cccctttcat tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga
    14101 agaatgacca gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca
    14161 cgcccatacg acggatccca gagttgatgc agttagacag cgcaaagtct ataatgcgga
    14221 acttaccgcc gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg
    14281 taccacgtcc tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca
    14341 ttaagtgatc gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa
    14401 cacacacaat ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc
    14461 agcgaatggg ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag
    14521 cgtggcgtta attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc
    14581 gttttgccac tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc
    14641 gtcgccttct tcccaccagc gattctccac caaagcggga atacgcttgc gcagatggat
    14701 taacgcggcg gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa
    14761 ttggttatcc tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc
    14821 ggccagtaac atcggcgtac cctgggagag caacaacgtt gttaacaggg cgtgaatact
    14881 gtcgcgccgc cgttcaacca ggtcaagaga accgcctaac ccttctttac catgattgtt
    14941 actgtaattg ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg
    15001 gttgaagcaa acgcagtcgc gaagcgtaaa accgtcatgc gcggtgacga gattaatcgc
    15061 ggcactcggc agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc
    15121 aaacgccccc agaggcaaat cataatgtag ccagaaacga cgggcagcat cgcggaaatg
    15181 atcgttccac tcggcaaaca gcggcgggaa atttcccacc tgataaccac caggagcgat
    15241 atcccacggt tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt
    15301 aaacaacggc gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa
    15361 gcggaaacca tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc
    15421 caccaccgcc ggatgactca aattgagcgt gttgccgcaa ccggtccagt tgtgataatc
    15481 gccgtcttct cttatccaat aatagctacg gttatcgatc ccacgcagcg agaataacgg
    15541 gccgtcgagg tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc
    15601 cgctttatgc agtgctttga ttgcatcgcg aaactcatcc agcgccgttt ctggcgagca
    15661 ggcatacgcc ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag
    15721 ccccatgcgt tgcagacgtg gttcactggc aaactgcgcc actggcagca gttccagcgc
    15781 ggtaatgccc aattgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt
    15841 gccacggatc tcgaccggga tctccgggtg caagtacgtt aatcctttga catgggcttc
    15901 ataaatgatg gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg
    15961 atcaaccacc actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt
    16021 atgaccggcg tgcagcagcg ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg
    16081 atcaatcaac aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg
    16141 aacgcgataa ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc
    16201 gccactgtgc cctggcaagt catagcgatg ttcctggcca ttggcgtcaa agacacacag
    16261 ttctacccgc tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg
    16321 cgcgccgagg ggagcgggtt tgccaatggc gagttgtgtc attctgcctc ccgaaccagc
    16381 cagatagtgg ccagcggtgg tagtgtcagg cttagtgaat gctgacgacc gtggctggca
    16441 atctcatcgc tgtgtaccgt gccgccattg cctgcattac tgccgtgata gtgcatggaa
    16501 tcggtattga ggatttcacg ccatttgcct ggctgattaa tgccgaagcg ataatcatga
    16561 cgcggcaccg gcgtaaagtt actggcaacg ataatttcgt taccctcttt atcgcgacgc
    16621 acaaagatca gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg
    16681 tcaaaatcca gttcatgcat tgctttatgg tggcggtagg tgaggttcag atcgcgcacc
    16741 agacgctgga caccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg
    16801 gcgtcatggt tccactcacg gccttgggca aattcgttcc ccatgaacag cagtttcttg
    16861 cccgggaatg cccacatcca gccatagtag gcgcgcaggt tagcgaattt ctgccatgcg
    16921 tcgcccggca tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac
    16981 ggcaggacga agttttcagt gtagttgtag agaatcccga aggtcagttt atcgtgatga
    17041 tactgacgat aaaccgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg
    17101 ttccacttgt accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa
    17161 tcggtagact cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg
    17221 gtattacgca agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc
    17281 cactccccct ctttacggct gtagtcgcga taaatcattg acgccaccgc atcgacgcgc
    17341 agcgcatcaa taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg
    17401 acttcacggc gaccatagtt gtagatcagc gtgttccagt cctgatgata gccttcacgc
    17461 ggatcgctgt gttcatacaa gttcgtgcca tcaaattcgg caagcgcaaa gtcatcagtc
    17521 gggaagtggc ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcggcatca
    17581 atgaaataac ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt
    17641 ggctgataac cccaactgcc atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg
    17701 gtaaagccca tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac
    17761 cagaaattgt tgtcggtgtg gcgacgccag gaacccaggt gaacttcata aatagagatt
    17821 ggcgcatcaa actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc
    17881 ccgcaaataa gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac
    17941 ttcagacgca agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc
    18001 gccccaggga taaacagttc ccagatgccg ctctctttac gcaggcgcat cgggtgacgg
    18061 cgaccgtccc agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccag
    18121 acagagaaac gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaggtttca
    18181 tacgggcgca ggtgagtacc ttcagataat agccaagcat ccatttcctg gattagcgga
    18241 ccaaaacggt aaggatcatc aatcaggttt tgctgaccat gccagacaac agccaactga
    18301 tagcggaaaa aattcttacg tcgcggaatg acgccgctaa agaatccccg tgagtcgaga
    18361 cactccagtt ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg
    18421 tcgggtaaaa gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa
    18481 aaaggatccg caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg
    18541 gacatgcttg tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg
    18601 ttgtgacatt tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct
    18661 cacttcaggt aaggctgtga atactcatgt attcagccac ccttaaagaa tagccaatgc
    18721 tctatttaac tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac
    18781 aggggcggca tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc
    18841 gacgcagcgg ctccgcggcc ccccacagca gctggtcgcc cactgtaaag gctgacagga
    18901 actctggtcc catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa
    18961 cggcagctgg ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc
    19021 acggattgtg cgcagccagc agttcttcca cggtcggaat agacacatct tttttcaatt
    19081 taatagtgaa tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta
    19141 ccggaattac ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt
    19201 cgcggctctg accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccagcg
    19261 gcacgccaaa gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt
    19321 cgatatcgag aatagcagag gacggggtcg cgagttcatc tgccacatgg ccatacagat
    19381 ggcccatctg ggttaataac tcacgcatat gtcgcgcacc accgccggaa gcggcctggt
    19441 aggttgcaac ggacacccaa tcaacaagat cattggcgaa taaaccaccc aacgacatca
    19501 acatcaggct tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt
    19561 cggtaatgac gtcctgattg acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca
    19621 gagacgatgc tgcgtcaatc cagtaacctt gccatccgct ttcacgaagc tttggataga
    19681 tttcgttggt ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct
    19741 ccagatcaaa ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctggc
    19801 caagctgaga agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca
    19861 tgcgttgcat gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca
    19921 taagcgtttt ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc tcttggcaca
    19981 tctttcacca tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc
    20041 catcagtaat gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg caattatcag
    20101 ggaatttgag ttatgaatga aatcatttct gcagcagttt tattgatcct gattatggat
    20161 ccgctcggaa acctgcctat tttcatgtcc gtactgaaac atactgaacc gaaaagacgg
    20221 cgggcaatca tggtgcgaga gttgcttatt gctctcctgg tgatgctggt gttcctgttt
    20281 gcgggtgaga aaattctggc atttctcagc ctgcgagcag aaaccgtctc catttctggc
    20341 ggcatcattc tgtttctaat cgccattaag atgattttcc ccagcgcttc aggaaatagc
    20401 agcgggcttc cggcaggtga agagccattt attgtgccgt tggcaattcc gttagtcgcc
    20461 gggccgacta ttctcgccac gctgatgttg ttgtctcatc agtacccgaa tcagatggga
    20521 catctggtga ttgctctgct gctggcctgg ggcggcacct ttgtcatcct gctacagtct
    20581 tcgctatttt tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg cctgatggga
    20641 ttgattctgg tgatgatggc aacccagatg ttcctcgacg gcattcgaat gtggatgaag
    20701 gggtaacgag gagagttttt cgaaaagaac ccggtagtgt cacaactacc gggcgaacaa
    20761 acttaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt gccgaggatg
    20821 gtttccatca tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt aaatttaccg
    20881 aacaaccaga aaccggcgtc gttaacgtgg ctgacaacaa tcgaaccacc agcgatacaa
    20941 atcgacagcg ccgccatttg cgcaccggag tagttcagtt gttcaataac cggcatcacc
    21001 agtcctaccg ccgttaaaca ggctacggtg gcagaaccct gaatgatgcg cactgcagct
    21061 gccagcacga agcaggtgat agcaatcggc aggcccatgc cagttaacgc ttcgcccagt
    21121 gccggaccta cgccagagtc aaccagcacc tgtttgaaca cgccgcctgc accaatcacc
    21181 agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac tttgtctttt
    21241 ggcatgccct gacgcattgc caggccataa atcgctacca gacaagcaac cagaatcgcg
    21301 gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc tggcacaaaa
    21361 cgcgcggcaa tggttttcag ccccaccagc accagcggca acaggatcag cgacaggctg
    21421 aatccgaaag atggcatttt gccttcgccg agatgcggtt cgctgatgtc gtcaggaata
    21481 tgcaactcca cgtagcggct gatgaaatta ccccacagcg gcccggcaat aatcattccc
    21541 ggaattgccg cacacaggcc aatcaggatc atccagccaa aatcggcgtt catctgcgat
    21601 gccagcagca ttggcgctgg ccctggcacc aggaacgccg ccgctgccgc cacgcctgcg
    21661 aataatggga ttaccagctt caccaggttc gtaccggtgt ggcgcgccat tgagaaagca
    21721 acgctaatca gcagaactat cgccacttca aagaacagcg gtagcgcaca gaccaacccc
    21781 gcaaggccga tggcataatg cgcgcggctg tgaccgaagg atttgagcat tttgacggca
    21841 atctgatcga ctgcgccggt ttcatgtaag atcttgccaa acatagctcc cagggcgaca
    21901 accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc tgcgatttta
    21961 tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccatcaa agccaggaaa
    22021 gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc tactgctgtt
    22081 aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc aatggtgctt
    22141 gccacaacac cttccagcgg ttgatcgata tccaccacca gtacatcggt ttcgtccgca
    22201 cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa gaaatggcct
    22261 ttgcgcgctt tcaagcgact ttcaatcaca tcaaaatcgc ctttcaggta gatgaaagag
    22321 agattcggat taccttcacg cagcaagtcg cgatagtgct ttttcagtgc agaacagacg
    22381 atcagcgaca ctttattggt gcgctgcata gcaaacgcgg cgtcgttcag cgcctgcagc
    22441 cacggtttgc gatcgtcatc atgcagcggt tcgccagacg ccattttttc gatattgcgc
    22501 cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc cacttcactg
    22561 gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg gtgatcatgg
    22621 ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta cgggtaactg
    22681 ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg tgccttaagt
    22741 gtataagtgt gagctacttc aaatttgtag gcttaaatag atccgcccgg tgacaaggtg
    22801 aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc cagcaggcgt
    22861 tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca cgctcgctaa ccgcggctcc
    22921 atcacctggc caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc aggaaccttc
    22981 aatcccagac gctgacattc aaacgccgcg ccgaccgcca ggtcatcgtt ggtacagaac
    23041 acgccatcca gctgcggata ttcccgccgc gcctggcgaa tcagttcaat accggaagag
    23101 taagaagaag attgctcaac catcacgcta tacggcacca gacctgcatc cagcatcgcc
    23161 tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgcgcgcc gagataggca
    23221 atgtggcaat gcccgcgagc aataatggcg gtggtcatct ggcgtgccgc ttcaaagtta
    23281 tcaaaaccga cggcgatatc gaggcacggc gactggctgt ccatgagttc caccaccggg
    23341 atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc ggtgaggatc
    23401 agtccatcga tattccatga gagcatggat tcgaggcgtt cttgctccat ttccggttta
    23461 taaccgtagt gcgccagcat ggtttgataa ccgtgcgcgt cggtgacgct ttcgattccg
    23521 cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc aatcgcccgg
    23581 ctggtagcgt tagagaggat atcgggagca cgattgggaa tatagcccag ttcatcaaga
    23641 gcggcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg taaaaaacgg
    23701 ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac gggtcttttc
    23761 tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca cggacgcatc
    23821 agaaccttcc cggggaaaac agtgcttgtt tacaattatt agatatacac taaatcaatt
    23881 gcatcgcatt gtgctaacga cgcggcaaaa ccccggaagc gtgcatcagc acgcgaccgg
    23941 agcgagcgag gaagcctgca aaaaggcagg cttcaaaaat gccgtggagc tttaaaccgg
    24001 cggcagatcg aacagtaaca cttcgctgtc gctatccgca tggatggaaa ttgcctgctc
    24061 atcccagatt gccagaccat cgctggtcga ggctttcacg ccgttaatgg tgacattgcc
    24121 tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg actgctcatc
    24181 tttcagcaac gcccaacgat acagttccat atcctgatgc actttcagcg aaccatctcg
    24241 cgcatccggc gagagcacca gctgtttgcc ctgcacggca tcgaagcgac gctgttcata
    24301 acgcggcgta atgccatttt cttcaggcat gatccagatc tgatacagat gcagacgctc
    24361 ggtgctgttt gggttgtact ctgagtgacg aatacccgta cccgcactca taatctggaa
    24421 ctcacccgcc ggaacctgct ctttattgcc catgctgtcc tgatgctcaa cagtaccttc
    24481 cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc cctgccctgc
    24541 ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt tcggatcgta
    24601 atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg catgaccacg
    24661 ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt tcgatgggct
    24721 tagtgtggac ccgatctgcc tgggatgata gagggtgaaa attgacccct ctgttcaaaa
    24781 aaattgaact attcgagggg ccatcaaact tacttagcga gagttactgt ggagggagag
    24841 gcttgctcaa atccgcgttc aaggatttcc agattggtaa gaacttcaga ttccttgacg
    24901 taatttggcg caccgtgggt gatggtttga tacaacgcat cataaacgcg cccgtaatcg
    24961 cccatctccg gcttcatctc ttctctgacc gtcacgccct cgtcattgac atactccagc
    25021 acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg gcataatatt agccttcagg
    25081 ctggtttcct gctggtcgat accgtattta ataaacgaac ctttcttacc gtgaacgata
    25141 aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt caggtcgcca
    25201 taaaacagtt gcgcttcaaa ggtgtcgtca ggattggctt tattacgcag gctgcggatg
    25261 tcataagcga cgtgatccgg gcgaccgaac agagaaataa tctggtccat cgtatgcaca
    25321 ccaaggccat aaaacgcgcc atcctgcggc agcccaggtt tggtttctgc caccgggcgg
    25381 taatagtcaa aatggctttc cacttcaaca atctctccca acttgccact ttcaatcgct
    25441 tttttcgctg tcaggaagca ggagtcaaag cgacgattct gatacggcgt gacggtcagc
    25501 cctttgcttt tcgccaacgc aaacagctcc ttcgcctggg caagcgtcgg ggtgaacggt
    25561 ttttcgacca gcacattttt cccggcttcc agcgcgcgtt tcgcgtactc gaaatggctg
    25621 tccgcgtggg tgcagacaac aaccagctta acatcgggat cgtttagtac ttcgtcgaga
    25681 tcgctggtga aatggatatg ggaataaatg ggagcctgtt cttccggctt cgcatggcga
    25741 cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg cagatggtaa
    25801 cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat ttcgtccttt
    25861 ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg agtgcgcata
    25921 acgaacacaa gcactcccgt ggataaattg agaacgaaag atcaaaaaaa aagccagcac
    25981 ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc
    26041 gagggtcttc ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt
    26101 tttgcaaaaa aatgcatttg actcgcattt gaaagtcaat gatgttgaaa gggacattta
    26161 ccccaaagag gacaaaggaa tgagtgagat agtaatacgc cacgcagaaa cacgggatta
    26221 cgaggccatc aggcagattc acgcccagcc ggaggtgtat tgcaacacac tacaggtgcc
    26281 tcatccttcc gatcatatgt ggcaggagcg actcgccgat cgtcccggca tcaagcaact
    26341 cgtcgcctgt attgatggag acgtcgtggg ccatctcacc attgacgtgc aacagcgccc
    26401 acgccgcagt catgttgccg attttggtat ctgtgtcgac tctcgttgga agaaccgcgg
    26461 cgtcgccagc gccctgatgc gagagatgat tgaaatgtgc gacaactggt tgcgggtaga
    26521 tcgcattgaa ctaaccgtgt ttgtcgataa cgccccggca attaaggtct ataaaaaata
    26581 cggctttgaa attgaaggga ctggtaagaa gtacgcattg cgtaatggtg aatatgtcga
    26641 tgcatattat atggcgcggg tgaagtaaga tagtgccctt tttctgagat ggataaaggg
    26701 tgtcattcaa aatcgacata ccttccttta aggt
//
LOCUS       DAZFAH010000046        26266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFAH010000046 DAZFAH010000000
VERSION     DAZFAH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.046"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHJWB_001817"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHJWB_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4313466.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHJWB_001818"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHJWB_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4313467.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHJWB_001819"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHJWB_001819"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4313468.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHJWB_001820"
     CDS             complement(1154..1621)
                     /locus_tag="ACHJWB_001820"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4313469.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHJWB_001821"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHJWB_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4313470.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHJWB_001822"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHJWB_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4313471.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHJWB_001823"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHJWB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4313472.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHJWB_001824"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHJWB_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4313473.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHJWB_001825"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHJWB_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4313474.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHJWB_001826"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHJWB_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4313475.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHJWB_001827"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHJWB_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4313476.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHJWB_001828"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHJWB_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4313477.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHJWB_001829"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHJWB_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4313478.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHJWB_001830"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHJWB_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4313479.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHJWB_001831"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHJWB_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4313480.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHJWB_001832"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHJWB_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4313481.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHJWB_001833"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHJWB_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4313482.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAVTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHJWB_001834"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHJWB_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4313483.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHJWB_001835"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHJWB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4313484.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHJWB_001836"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHJWB_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4313485.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHJWB_001837"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHJWB_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4313486.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHJWB_001838"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHJWB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4313487.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHJWB_001839"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHJWB_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4313488.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHJWB_001840"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHJWB_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4313489.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHJWB_001841"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHJWB_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4313490.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEVAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHJWB_001842"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHJWB_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4313491.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     TEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHJWB_001843"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHJWB_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4313492.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHJWB_001844"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHJWB_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4313493.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHJWB_001845"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHJWB_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4313494.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHJWB_001846"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHJWB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4313495.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHJWB_001847"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHJWB_001847"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4313496.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHJWB_001848"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHJWB_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4313497.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHJWB_001849"
     CDS             15761..16129
                     /locus_tag="ACHJWB_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4313498.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHJWB_001850"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHJWB_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4313499.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHJWB_001851"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHJWB_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4313500.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHJWB_001852"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHJWB_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4313501.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHJWB_001853"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHJWB_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4313502.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHJWB_001854"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHJWB_001854"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4313503.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPETQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHJWB_001855"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHJWB_001855"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4313504.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHJWB_001856"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHJWB_001856"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4313505.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHJWB_001857"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHJWB_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4313506.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRLISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHJWB_001858"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHJWB_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4313507.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHJWB_001859"
     CDS             23308..23850
                     /locus_tag="ACHJWB_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4313508.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHJWB_001860"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHJWB_001860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4313509.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHJWB_001861"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHJWB_001861"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4313510.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHJWB_001862"
     CDS             25247..25504
                     /locus_tag="ACHJWB_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4313511.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHJWB_001863"
     CDS             complement(25480..26034)
                     /locus_tag="ACHJWB_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4313512.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6737 a         6241 c         6806 g         6482 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gcaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg gtagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg caaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgctttgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggta accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggta atgtgattaa cctgtctctg gggttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaagtct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagt tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt tcgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtggttcacc caacgttgac agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgctacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccggg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagatcactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaatat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagtatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgc catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcacgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcaacgga gagaagcaat
    15961 tcagcttctg gatattctca aaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactggca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagtcgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgcgtagag aaaaataaga aggggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcagga gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgcagg tgcggctgct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca
    17101 attaataagc ccagaggtgg catgatgata tcggcaacca gtgaagatac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacatgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaacgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttca
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gcggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcattac caatcagcgg gccgccataa taggctttaa ccacagccag gctgacttta
    18301 ccctcagcga agtttaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagcg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgacccgcag gtcaaattta tcctgtaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggtag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcacaa agttctgcat cagcggtacg ttgcaaaaaa
    18901 gctttaatct gcaggctatt ttcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgccttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgtttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caaaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagacgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg acgcttaatt
    19741 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gcaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgccagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagaccat ctgcctccag ttcaaatggt ttaccgtcgc
    21241 ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc atacatcgtc tcgaacatat
    21481 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agatatttgg ctgcgtttaa tgagcatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gtgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agctgcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcct tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcagatt caggtgctca
    23101 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
    23161 aactggatga tctgaaatgg gttatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgtcggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa ccaatagctg gagaatgccc tgagtgtcat
    23761 tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gttgggtgcg atcctgatag cgaaacagca gtgatgcgac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tctaatcgcc gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcaacaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttagtgct ggtggtaaag
    24601 gtgcgaatgt gacggtacct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcttgc
    24721 agggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtccgggttt acgtattctg cttatcggcg ctggtggagc atctcgcggc gtactactac
    24841 cactcctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatctctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aaatacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aggatggtcg gaacaa
//
LOCUS       DAZFAH010000047        25418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFAH010000047 DAZFAH010000000
VERSION     DAZFAH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.047"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..616
                     /locus_tag="ACHJWB_001864"
     CDS             197..616
                     /locus_tag="ACHJWB_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4313513.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            616..1884
                     /gene="umuC"
                     /locus_tag="ACHJWB_001865"
     CDS             616..1884
                     /gene="umuC"
                     /locus_tag="ACHJWB_001865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO4313514.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1930..2460)
                     /gene="dsbB"
                     /locus_tag="ACHJWB_001866"
     CDS             complement(1930..2460)
                     /gene="dsbB"
                     /locus_tag="ACHJWB_001866"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4313515.1"
                     /translation="MLRFLNQCSQGRDAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2606..4147)
                     /gene="nhaB"
                     /locus_tag="ACHJWB_001867"
     CDS             complement(2606..4147)
                     /gene="nhaB"
                     /locus_tag="ACHJWB_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4313516.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4368..5087
                     /gene="fadR"
                     /locus_tag="ACHJWB_001868"
     CDS             4368..5087
                     /gene="fadR"
                     /locus_tag="ACHJWB_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4313517.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5139..6671)
                     /locus_tag="ACHJWB_001869"
     CDS             complement(5139..6671)
                     /locus_tag="ACHJWB_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4313518.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7001..8299
                     /gene="dadA"
                     /locus_tag="ACHJWB_001870"
     CDS             7001..8299
                     /gene="dadA"
                     /locus_tag="ACHJWB_001870"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4313519.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8309..9379
                     /gene="dadX"
                     /locus_tag="ACHJWB_001871"
                     /pseudo
     CDS             8309..9379
                     /gene="dadX"
                     /locus_tag="ACHJWB_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(9766..11502)
                     /locus_tag="ACHJWB_001872"
     CDS             complement(9766..11502)
                     /locus_tag="ACHJWB_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4313520.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11597..12511)
                     /gene="ldcA"
                     /locus_tag="ACHJWB_001873"
     CDS             complement(11597..12511)
                     /gene="ldcA"
                     /locus_tag="ACHJWB_001873"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4313521.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTINGHPVLKM"
     gene            12611..13222
                     /gene="emtA"
                     /locus_tag="ACHJWB_001874"
     CDS             12611..13222
                     /gene="emtA"
                     /locus_tag="ACHJWB_001874"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4313522.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13224..13958)
                     /locus_tag="ACHJWB_001875"
     CDS             complement(13224..13958)
                     /locus_tag="ACHJWB_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO4313523.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14159..14413
                     /locus_tag="ACHJWB_001876"
     CDS             14159..14413
                     /locus_tag="ACHJWB_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4313524.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14591..15031
                     /gene="ycgY"
                     /locus_tag="ACHJWB_001877"
     CDS             14591..15031
                     /gene="ycgY"
                     /locus_tag="ACHJWB_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO4313525.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15110..16807)
                     /gene="treA"
                     /locus_tag="ACHJWB_001878"
     CDS             complement(15110..16807)
                     /gene="treA"
                     /locus_tag="ACHJWB_001878"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4313526.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17127..18545)
                     /gene="dhaM"
                     /locus_tag="ACHJWB_001879"
     CDS             complement(17127..18545)
                     /gene="dhaM"
                     /locus_tag="ACHJWB_001879"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO4313527.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18556..19188)
                     /gene="dhaL"
                     /locus_tag="ACHJWB_001880"
     CDS             complement(18556..19188)
                     /gene="dhaL"
                     /locus_tag="ACHJWB_001880"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO4313528.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVCGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRTSYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19199..20269)
                     /gene="dhaK"
                     /locus_tag="ACHJWB_001881"
     CDS             complement(19199..20269)
                     /gene="dhaK"
                     /locus_tag="ACHJWB_001881"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO4313529.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20497..22416
                     /gene="dhaR"
                     /locus_tag="ACHJWB_001882"
     CDS             20497..22416
                     /gene="dhaR"
                     /locus_tag="ACHJWB_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO4313530.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVISTTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22516..25383)
                     /locus_tag="ACHJWB_001883"
     CDS             complement(22516..25383)
                     /locus_tag="ACHJWB_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4313531.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSYISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTAAAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDEMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         6281 a         6334 c         6563 g         6240 t
ORIGIN      
        1 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
       61 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac aaatattgat
      121 agcctgaatc agtattgatc tgctggcaag aacagactac tgtatataaa aacagtataa
      181 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      241 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgttga
      301 acagcgcatc gatctgaatc aactgttgat ccagcatcct agcgcgactt acttcgtcaa
      361 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      421 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      481 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
      541 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
      601 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
      661 acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa taatgacggt
      721 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
      781 ttcaaacaaa aagatctgtt tcgtcgctgt ggcgtggttt gctttagcag caattatgag
      841 ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc gccccgcgtc
      901 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
      961 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1021 ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc ggcaaaaaaa
     1081 tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta
     1141 atgtctgctc tccccgtgga tgaagtctgg gggattggac ggcggatcag caaaaaactg
     1201 gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg gtttatccgt
     1261 aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggcga accctgtttg
     1321 caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc gtttggtgaa
     1381 cgcatcacgg attatccatc gatgcggcag gccatttgta gttacgctgc ccgggcggcg
     1441 gaaaaacttc gcagcgagca tcaatattgt cggtttatct ccacatttat taagacgtca
     1501 ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact gctgacgccc
     1561 actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc catctggcaa
     1621 gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag tcagggagtc
     1681 gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca attgatgacg
     1741 gtaatggata cactgaatgc taaagagggc agaggaacac tctattttgc cgggcagggg
     1801 atccagcaac aatggcagat gaagcgtgcc atgctttcac cacgttatac aacgcgaagt
     1861 tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgctcc cgcaggagcg
     1921 ctgaagggat tagcgaccga acaggtcacg ttttttcgct ttaaacggct gggaaatcac
     1981 caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact gcggcatttc
     2041 cagacctaaa aaatcccact gacgctcggc acaatcgcca gaggcgacaa acacttgcgg
     2101 cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac aggtggcaaa
     2161 cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc
     2221 actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg cgatcgcgcc
     2281 aatcagcgca gcacccagaa cgccgaataa cgcacaacgt tcataaatac agagcacgca
     2341 aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg ccagagcagt
     2401 aaacgccatc aacagccacg cgtcccggcc ttgtgaacat tggttcaaaa atcgcaacat
     2461 aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca
     2521 ccaggaggca gcaaaaatag gaagcaattg gacaattatc gggcgaaaag gcaaaccggg
     2581 catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc
     2641 attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt gtcagtacga
     2701 gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc aatggcgcga
     2761 gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acggagggca
     2821 gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc
     2881 cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg
     2941 aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca tgttccgatg
     3001 cctgcaacac aaactgaata attggcgaaa acagttgttg gtcgataatc accgcgacta
     3061 ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga
     3121 tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgact gacaaaccaa
     3181 tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg ccaataatcg
     3241 cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa
     3301 actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca aaccaacgca
     3361 gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg gtcaccggcg
     3421 acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg atcaggttct
     3481 gtggttcgcc caccatggtc atcacgccgc ctaatgcagt gccgacaccg gcatgcatca
     3541 tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat
     3601 gactatcgtc ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg tgataaatac
     3661 cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg
     3721 cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg gagcgaatgc
     3781 ttaacagcaa acgggtaaat atgaacagca acagctgttt cataaaatag atacccgcca
     3841 ccataaacat caataacagc aagacttcaa gatttgccgc cacctcttca cggatgtgtt
     3901 ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg gggagtagcg
     3961 ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc aaccagcccg
     4021 cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg atgagggcga
     4081 gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg ccccaggaga
     4141 tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg tttaaacgca
     4201 aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa agagatgcgg
     4261 aaaatgaagc cttgatccct ttttcttctt tttgtctgct atcagcgtag ttagccctct
     4321 ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg
     4381 cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct
     4441 tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc
     4501 gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac
     4561 atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat atccttgaaa
     4621 cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc
     4681 gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc
     4741 aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg
     4801 attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgattc
     4861 ttaacgggat gaaagggctg tatacgcgta ttggtcgtca ctatttcgcc aatccggaag
     4921 cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc
     4981 acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc
     5041 ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta
     5101 aagagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc
     5161 gggcaacgtt ccagcaactc gatgctgccg tcttcgtttt gctgttcgag catcacatca
     5221 aatccccaca ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg
     5281 cgattatgtg gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag
     5341 atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc
     5401 tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcatca
     5461 tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata
     5521 aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg
     5581 gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata
     5641 tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta
     5701 taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc
     5761 gttactttcc cttcatcata cagatggtta aggatggtat agtgccagaa ggtcgcccag
     5821 ccttcgttca tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca
     5881 atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag
     5941 agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc
     6001 tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga
     6061 cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc
     6121 acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc atcaacgcca
     6181 taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa
     6241 ctggcgtcgg tccaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag
     6301 caggcgtgtg ccatcaccag cgcctgcatg gtaatggtgt tctcttccat caggtaagcg
     6361 atacacgggt tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac
     6421 agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg
     6481 ccgacgctgg agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg
     6541 taggtatcca gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc
     6601 aggtcgaacg tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt catagaatcg
     6661 atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc
     6721 aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt
     6781 attatcagag tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt
     6841 tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc
     6901 accataaaaa agccgtatat tgaataatat tttcaactga gttatcaaga tgtaattaga
     6961 ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg
     7021 aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac
     7081 cgtcattgat cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat
     7141 ctcccccgga tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg
     7201 gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa
     7261 atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg
     7321 gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa
     7381 tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta
     7441 tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct
     7501 ggaatccagc cgcctggcgg aagtggagcc cgcgctggca gaagtagcgc acaaactgac
     7561 gggtggtctg caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct
     7621 ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact
     7681 gctttgcgac ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga
     7741 tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca ttgttgatat
     7801 tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc
     7861 gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg
     7921 cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg
     7981 tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca
     8041 ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg
     8101 tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc
     8161 ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata
     8221 tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt
     8281 acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct
     8341 gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgcgcgtctg
     8401 gtcggtggta aaagcgaacg cttacgggca tggtattgag cgtatctaga gcgcgctcgg
     8461 ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac gtgagcgcgg
     8521 ctggaagggg ccgatcctga tgctggaagg atttttccat gcacaggatc tggagattta
     8581 tgagcagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa
     8641 tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga tgaatcggtt
     8701 gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt
     8761 tggcgagatg accctgatgt cgcattttgc cgaagcggaa catcctgatg gaatttccag
     8821 cgcgatggcg cgtattgagc aggcggcgga agggctggag tgtcggcgtt cgttgtccaa
     8881 ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc ctggcattat
     8941 tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg gattacgtcc
     9001 ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg gtgagcgtgt
     9061 gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg
     9121 gtacgccgac ggttatccgc gccacgcgcc taccggtacc cctgttttag tggacggcgt
     9181 gcgcaccatg acggtgggga ccgtctcgat ggatatgcta gcggtcgatt taacgccttg
     9241 cccgcaggcg ggtattggta cgccggttga gctgtggggc aaggagatca aaattgatga
     9301 tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg cgctacgcgt
     9361 cccggttgtg acggtgtaac ttgttgtaag ccggatcgga ggcaacgtct tctgggtgca
     9421 aaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
     9481 ccatgatagt gtccattaaa aggatggaca ctatttcccc ggaacctgaa ctcaccgcac
     9541 aggcgttcta cataaaacgc ttacgcttca ttgttgactc gaactcgact tcagataaat
     9601 cacgctttac ccttgatgga gcctgtacat agatttgtgt aattgcctga ttttgatatg
     9661 ttcaattcaa catcaaatga aggttaaatt atggacgaca aacaattgca ggctcaggct
     9721 gcgttcagca aagcatcgca accggcgata gatgcttcat taaatttaag attcagcttc
     9781 ctcttcagcc acccgtacgc caatcttcaa cacttcatta tcttctttct cggccaccgt
     9841 ccagatcatc ccggcaaact ctacctggtc accgacaacc ggtgccgcgc ctaacaactg
     9901 ctggacaatt tcacccagcg tttgctgctt atcacgatat tctcgcccgt cttccaggcc
     9961 atatatcagc gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa
    10021 gcgttgatcc agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg ccggaagatc
    10081 gcgttcccga ccaattacac acaacacatc gccttcgcgc agtcgggtgc tgccggtagg
    10141 atgaagcaac tggttatcac gaaacagtgc cgcaatacgc gtctcttttg gcatatgcaa
    10201 atcgcgcagt gccgcgccca cgcaccattt atcggcactc aattgataaa caaactgctc
    10261 ccacggattt tccggatgaa tatccaggcc aacgcgtgac accggacgtc ccactggcgg
    10321 aaccaccact ttggcttttt tagccgccca cgagagtgat gttccctgca agagcagtga
    10381 aaccagaacc acaaagaagg cgacattaaa gaacagacgt gcattctcca gccccgccat
    10441 catcgggaac acggccagga tgattggcac cgcgccgcgt aatccaaccc agctgataaa
    10501 cacgcgctca cgcagattga aaccgcggaa ggggagcaat ccagcaaata ccgaaagggg
    10561 acgggcgaag aatatcatcc atgccgacaa aatgagcgcc ggaatggcaa taggcagcag
    10621 atcgcttggg ttaaccaata gccccagcac caggaacatg gcgatttgcg ccagccaggc
    10681 gaggccgtcg aaattttgca ggatgccgta gcggttgcga atcggccgat tacccagcag
    10741 aaaaccgcac agataaacag ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa
    10801 aattaaaata ccgccgctta atgccagcaa tggatataat ccggcaggca gggcgatgcg
    10861 attaatcatt tgcagcagta aataaccacc gccaagtccg atgacaatcc cgaggccaaa
    10921 ttgctgcaga atatcgacca cgaacatcca actgacactg ctttcatgtt gctggatcat
    10981 cgcaatcagg gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc
    11041 cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc
    11101 agcatcggta gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa
    11161 cagccacgcc gccatcatgc cggttaaacc agaggtgata agcacgccca gcgtcgccag
    11221 cgacagtgcc ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc
    11281 gagcaaaata atcgccagtg ccaggttact taccatgtag gcgaaggggt aattatcaaa
    11341 cgggataccg ccgacgccat cgactcctgc cagcatgccg atcgccagaa aaataaccag
    11401 aataggaatg ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat
    11461 ggatcctaag atgaaaaggc taattattgt tgtggcatcc aacggtcggt tactcctgat
    11521 tacgctgtct cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc
    11581 ctgaagcgtg atttttttac attttaagaa caggatgacc gttaatcgtc aactgagtgc
    11641 cttcccgggt gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt
    11701 catgaccaaa atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt
    11761 acactgactc caggttgtaa cctgcgtcat aatcattggg cgtgctgccg ctaaagctac
    11821 cgagaataat cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac
    11881 gttcgacgcg gaaagggtgc tcgttaatat cttccagcac cagaataccg ttctcaattt
    11941 ttggcatcca cggtgtacca atcagtgaaa tcagcatcgc aagattgcct ccccacaacg
    12001 tgccttcggt ctgacatgtc gggccttccc cttgccattc aatggtgaag gtttcattgc
    12061 gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag ttcatccgcg ccaaagtttg
    12121 ctaccagcat ggggccgcta aaggtgatga cattgccttg cgccagaaga ccgcactgaa
    12181 tggcggtaaa atcgctatgc ccgcatatga gcaacgggtc atattgttgg cgagcaacca
    12241 gtgcctgcca gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca
    12301 gcacgatggt gttgggggtg gtcagtctgg caagggaatt gagatcttcc aggcgctccg
    12361 tttccgtacc cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacctgat
    12421 gcccctcgtc ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgcagtaac
    12481 ccgatggggc aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa
    12541 atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag
    12601 gatagaagaa gtgaaattga gatggtttgc ctttttgatt gtgttattag cgggttgttc
    12661 atcaaagcat gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat
    12721 gcagtggatg ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat
    12781 cacggcgatt atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc
    12841 cattggtttg atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg
    12901 ctggagtggt gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg
    12961 ggcggcttac ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt
    13021 actgcaatat gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt
    13081 ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct
    13141 cgatcacgta gcgcgaaatc atcctgcgcc gcaggctccg cgctatatct acaaacttga
    13201 gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc tcgggcttct
    13261 cgctcgagag agaaaataat ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga
    13321 aaacggaagc tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc
    13381 tcgctgatgg agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact
    13441 tcaatctgag cgaagcgcat gccttcttgt aattcggcag gctttgctgt ttccagtaat
    13501 gcgcccatgc cgcctaacga caaatcatac aggcggaaac gtaacgtact gttatccgcc
    13561 agtttggtct ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt
    13621 cgttgtacaa accataaggt gggaggcggt acggtgataa atgccggaag ctgcaagtat
    13681 tcactctgct gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta
    13741 atggtaatgt gctgtgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc
    13801 agcaccagtt tatccggggt tattgccagt attttgctga tcagctgccc gccattccaa
    13861 ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac
    13921 ggattttgtt tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca
    13981 aactgtcttt ggtttatcgg tcacagttaa caaaacttaa tacaaatgtg tgaatatttt
    14041 ttacatgctg ttcttaaatc agccgacata cgcccagcat cgatcccgcc cttgcctata
    14101 cttagagcgt tgacgtaagc atttcttgcg tccggttcat cgaaacgagg gcctgaacat
    14161 gggaattatt gcctggatta tttttggcct gatagccggc attatcgcca agctaatcat
    14221 gccggggcgt gatggtggtg gatttttcct gacctgtatt ctcgggatag tcggtgcggt
    14281 ggtcggcggc tggctggcga ccatgtttgg cattggcggc tccatcagtg gttttaatct
    14341 gcacagcttc ctggtggcgg tagtgggcgc tatcctggtg ctgggcgtat tccgcctcct
    14401 gcgaagagaa taagattttc ataaggcgga tagcgataca gatgccgcta tccgctttca
    14461 catcagaacg tatactcgac acctgcttta cgggtggaac aaatcaatat agcacttaga
    14521 tgcccgataa agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt
    14581 tgtttttggt gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa
    14641 tggggcggat agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc
    14701 ttctggcaga ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg
    14761 gtctgaattc tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa
    14821 tttaactcca gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc
    14881 aattgatgct ttatatagcc aacgtcggcg taaatcgccg ggtgatgaaa gtgaaacact
    14941 tacccgtaga aatgacgcta tcttcggcaa ccacgtatgg cagacatttg cgcaatactt
    15001 cccgccagga cttgaaaaac cgtctgtgta gaaaaaacca tagatatggt gttatctgcc
    15061 gacaacgcca tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg
    15121 gttgtgcctc tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg
    15181 gaacattgtc acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc
    15241 cattggtcca gccaaagcca tcctgtaatg gatattcgcc accgccgccc cccgttccgg
    15301 tggtgctgac atcatatttt tccaccagct ttttctcccg gtcataggtg tgctgaacat
    15361 tggtcaggaa gtgccagcta atgtccatcg ccacctcttt ttgcccgtag ttttgtaatc
    15421 cttctgtcgc gacccactgt aacggtgccc agccatttgg cgcatcccat tgttgcccac
    15481 ttttcaccga cgtggtgttc aggccgccgg gttgcagcag atgtgttttc gtcgccgtcg
    15541 ccattttgtt ggcgcgatct ttcgctgccg cattgacgta cagcgggaac agggcggccg
    15601 cggttaactg attgcgcact ttatgacttt tcaggtcgta atcggcatac cagccttgtt
    15661 gatcgttcca catgtatttt tcgatccctt tttgacgggc atttgccagc gtttcgtact
    15721 ggtttgccat cgcgttatct ccggcagctt tgctggcgcg ggcgaggatt ttttccattt
    15781 taaacatcag gctgttcaga tcgaccggta cgatgctggt ggtgcgtaag gtatttaact
    15841 gctgcgggtt gtccatccag cgcgagctga aatcccagcc agacgcagcg gcagagcgca
    15901 ggtcgcggta aatttcagtg gcaggtcgat tcggattgct tttggcggtg gcaatatctt
    15961 ccacccatga ctctggtcgt ggcgtatcgc gatcgtccca gtagcggttg agaagggtac
    16021 catcctgaag ttttacaacg cgtttttcct gttgtccggc ttgcaggttt tcaacaccgt
    16081 ccatccagta agcatattct ttttgcattt gcggcaggta ttgcttcaac gcggcatcgc
    16141 cttcatgctg cgccagtaac tctaccatca gggcaaagaa gggcggttgc gagcggctta
    16201 aatagtaact gcggttgccg ttgggaatat gaccgtaagt gtctatttca tgagcaaaat
    16261 tggccaccat atccgcgact ttatcccagt gaccgctttc ggcaagtcct aacatggtga
    16321 agtaactgtc ccagtaatat acctcgcgaa aacgtccgcc cggcacgaca taaggttccg
    16381 gcagcggtaa cagagaatcc catttttcgg tgttttcggt agaacgcgtt aataccggcc
    16441 aaagtccgtc aatatgttcg cgcagtgact gtccctctgg cggaacatat ttctcgccct
    16501 ctttcggcag ggtgaaattg acgttaacga aatggcgcag atcaaatccg ctctggtttt
    16561 gctgcatccg ataatcagca aggatcatca gcggatcgct gttcggcacg gcatcggcaa
    16621 aggttttttg gtccggaaaa agtttggcgt tttgcacatc attaaacagc ggccctaata
    16681 aaatatcagg cggctgtggt gttaccggtg tttcttctgc ctgcaccgat agcgcagcga
    16741 aacacaaaaa gatgcaggct ggaattaacg ccattttttg cgggcgagaa ggtgcggggg
    16801 atttcatcaa tcattctcct ttggcgaaac cgaataaaac ggtattcaag tatcagaaaa
    16861 ccttagttca ggatcgcact agctgcatga tcgaaatctc attctgcgaa ctgtcaggca
    16921 ttttagaaaa gggaattaaa gggataaata acggcgaaat agagtgatca aataacgtct
    16981 gtttattagc cagccagcga catttggttc gcgattagcg aagaaaaatc atcataaatt
    17041 ggtaaaaaat ataacaggta gttaaaaaac atcagtgctg ggtaaattgc cggatgacat
    17101 cagaacgatg ccatccgaac ggtggcttaa ccctgacggt tgaaacgttg cgttttaacg
    17161 tccagcgtta gcgtttcttc tggttgtatc gcatacagtt tctcaccctg ctggcaaatc
    17221 cagccaatcc ccagttcacg ggcgattagg gcgctgtggg ataccggact tccggcgcta
    17281 aggcagatac cttttacaac cgccggatcc agttgcagta ctgtggaagg ataaatgttc
    17341 tccgccagta gaatagttgg cgagttaaac tgcgggagtt cttctttcgt ttgggtcagg
    17401 tggaccaggg tgcgatgcag aagatcgtcc acatcaatat agcgagcttg tagatattca
    17461 tcatccagtt gctggtattg ctggctaagt tctttaagaa cttgctgcca ggcatattct
    17521 gccgtgcaat gttcatgctg aaggagttcg cttgccgccg ccagcagttc cggatcatct
    17581 aacagtgtat ggtgaccaga aaagattgcg gcaatatcgt caagcccgct ggcttctgct
    17641 ttcgctgtta acgtcatcag atctaataac gtgaagtcaa tagcctggcg taatcgatct
    17701 tgttcttctt ccacggtcag ggttgatttt gcctgtaccg tacataaaac tggttgataa
    17761 taaaaggctt tacccgaaac aggcggaacg ggacgcagag taggtggagc gacttcctcc
    17821 gtttcaccaa agttatcttc agccagctga cggaaagcga tcagtgcctc ttcagcttct
    17881 ggccctttcg caatcaggcg cagcgtatcg ttatagcgaa cttgtagtaa cgcaatctgg
    17941 ttaatactct ctggtgtgac gcatttgccg tttttttcca gcaacatatc ggcattaaat
    18001 gtcgataagg tataaaccag ccgggaggcc ggacgtacat gcaggccgtt acggtttttt
    18061 atgaccaccg ccagagaacg ggcttcttca tcgtacgcag gacatgtgtc agagatttca
    18121 gtgtcggagg acggtaaacc cagttgttca cgtttggctt ccagcgcatg catagcgtca
    18181 aagataactt tgtcgatatc cgctcccgag gccgcgctga ccgttgctgc cagtgtacct
    18241 tcgaccaacg gcgcagcaca caaacgtact tttgcggcga tcttgggagc cagcaattcc
    18301 agcgcagttt cagcactcaa taatgcgcta cccatatcca tcatgaccag cacatggtcg
    18361 gcatcagcaa cagattcgat ggcctccatc actttgacgg catcggtacc aataggattt
    18421 tgtggatcgt caattcccgc ggcaatggcg attttacaac tatcactcat taacatctga
    18481 cgggctaatt caccgacacc ttctcccagt cggctgctat gtgaaactat gaccaggttt
    18541 accatcacca attccttact cttttgcggc taacgccaac atttgcatca taaacatcac
    18601 cgaggtcgcg ccgggatcct ggtgaccaat actgcgttca ccgagataac tggtgcggcc
    18661 tttgcgggct tgcatcgtaa tcgtactttg tgcagcggat tcggcgattc tactggcagc
    18721 ttcgagcgcc accggaacag agagattttg ctcgctggac tgacgtaacg attccaccac
    18781 cggcacccac acatcacaca tggttttatc gccaggttcg gctttcccgc gactgattac
    18841 gccgtccgcg ccatcgcgga acatctgata aagctcttcc agtgtcaggc tttgccgtgc
    18901 ctgggtcgcc tgtgcggcgc ggataaagaa ggtaccgaac agcggaccac tggcaccaca
    18961 gacgctggaa agcagcgtca taccggtatt cttgagaatg aaaccgatat ctttatctgc
    19021 gatagcaggg agtttttcca ccactttgct aaagcctcgg ttcatattta gcccgtggtc
    19081 agcgtcgcca atttcgcgat ccagtccggt aagatactcg ctctcggtgc tgaaaatatc
    19141 gccacaacga gtgagccagt taacaatttg agttctgctc agtgacattg ctttctcctt
    19201 atttacccca attaagggcc ggggtgtgga ccggggcgtc ccagagtgcc agcgtttcgt
    19261 catcaacttt cagtaaggtg attgagaaac cggtcatatc cagtgaggtg cagtacgcgc
    19321 caattaaatt acgttcgata gtcaatcccg cttgctggca acgtgtggtc aggcggttat
    19381 agacgccgta cagctcagaa agcggagttg cgccaagatt gttaaccagc gcaatcaccc
    19441 gatcgccaga ctggagcggt tgtttggttt gttgttcttc ctgccaactg ccttgttgat
    19501 aatcccagaa acgcaaagtg cgatggtatg agccattttc cagcagggtg tcgaacattt
    19561 catcgacggt ttgatcaagg gaagagaagg agcggcggtc aatacccggc tcaccatgaa
    19621 tgccgacgcc aaactccatc tcattatccg ccagggtaaa agaaggtttg cccgcggcag
    19681 gaacggtaca ggcaccgaga gcgataccta ttgagtggcc ttgattattc aacttacgcc
    19741 ccagttccgc acaggcgtcc agtgagtcgc cacgctccgc cgctgcgcct acgagttttt
    19801 caattaatac ggtgttggca acgccgcgtc gcccggcggt ataaagactg tcttttaccg
    19861 caacgtcgtc atcaatgacc acagtggtca cttttacgcc gctatcgtgc agtaactcgg
    19921 tcgctgtttc aaagttaaga atatcgccgg tgtaattttt gataatcaac agtacacctt
    19981 cgccgccatc aatttgcatg gagcattcaa agattttatc gggcgtcggt gaggtgaaaa
    20041 tttcgcccgg acaggccccc gaaagcatcc cctgaccgat ataaccacag tgcatcggct
    20101 cgtgtccgct gccgccaccc gacagcaggg cgacttttcc tgcaacaggg gcatcagctc
    20161 gggtgacata caccggatcc tgatgcagtg tcagcgatgg atgcgctttc gccagtcctg
    20221 ccagttgttc gtccagtacg tcttgcacat cattgatcaa ttttttcatt attttgctcc
    20281 agcaattacg gtagggcatg gatgatgttc aacgacacgg cgaccactga ctgccgatga
    20341 atccattgtg catcaggcaa gggaaaagaa aattagccca aattatgttt catagtgaaa
    20401 catatgcttt aatgaatgtt ccatattgaa acttttacgt gtattaatac ttaaaattgc
    20461 gagccggaac accttttgtc ataagggatg cgggatatga gtggcgcttt taacaacgat
    20521 ggtcggggca tatctccctt aattgcaacc tcctgggagc gatgcaataa gctgatgaaa
    20581 cgggagacat ggaacgtacc acatcaggcc cagggcgtga catttgcttc tatttatcgg
    20641 cgtaagaaag cgatgctgac gctcgggcag gctgcgctgg aagatgcctg ggaatatatg
    20701 gcaccgcgag agtgtgcgct gtttatcctc gatgaaaccg cctgcattct cagccgtaat
    20761 ggcgatccgc aaaccttgca gcagctaagt gcactgggat tcaatgacgg cacgtattgc
    20821 gccgagggaa ttattggtac ttgtgcgcta tcgttagcgg ctatctctgg tcaggccgtg
    20881 aaaacgatgg ccgatcaaca tttcaaacag gcactctgga actgggcctt ttgtgcaacg
    20941 ccgttgtttg acagcaaggg ccgattgacg ggaacaatag cgctggcgtg tccggttgag
    21001 caaactaccg cagctgattt gccgttgacg ttggcaatcg cccgcgaggt cggaaattta
    21061 ctgctgacgg acagtttgct cgctgaaact aaccgtcatt taaatcaact taatgccctg
    21121 ttagaaagta tggatgatgg cgtgattagc tgggacgagc agggtaattt gcaatttatt
    21181 aatgcccagg cggcgcgggt cttgcgcctt gacgcgacgg caagtcaggg acgggcaatc
    21241 actgaactct taacgttacc cgccgtattg caacaagcaa taaaacaggc acatccgctc
    21301 aaacacgtag aagcaacctt tgaaagccag caccagttta ttgatgcggt gataaccctt
    21361 aaaccgataa tagaaacgca gggaaccagc tttattttgt tgctccatcc tgtggaacag
    21421 atgcggcagt tgatgaccag tcaattagga aaagtcagcc ataccttcgc tcatatgcca
    21481 caggacgatc cgcaaacccg ccgcttgatt cattttggtc gccaggcggc gcgcagtagc
    21541 tttcctgtcc tgctttgtgg agaagagggc gtgggcaagg cactgctaag tcaggcaatt
    21601 cataatgaaa gcgagcgtgc tgcaggtcct tatatcgccg tcaattgtga gttatatggt
    21661 gatgcggcac tggcggaaga atttattggt ggcgatcgca cggacaatga aaatggccgt
    21721 ctgagtcggc tggaactggc gcacggcggc acgctgtttc ttgaaaagat tgaatatctg
    21781 gcggtggagt tacagtctgc tttgcttcag gttatcaagc agggggttat cacgcgactg
    21841 gatgcgcggc gtttaatacc aattgatgtc aaagtgattt caacaacgac cgcggacctc
    21901 gcaatgctgg tggaacaaaa tcgttttagt cgccagctgt attacgcgct gcatgcattt
    21961 gaaattacca tcccgcctct gcgtatgcgg cgtggcagca ttccggcgct ggtgaataac
    22021 aaattacgca gtcttgaaaa acgcttctct acgcggctga aaattgatga cgatgccctc
    22081 gctcgcctgg tttcttgtgc atggccaggc aacgattttg aactttacag cgtcatcgag
    22141 aatcttgctc tgagtagtga taatgggcgc attcgcgtca gtgatttgcc ggaacatctg
    22201 tttaccgagc aggcaacaga tgatgtcagc gccacccgcc tttccaccag tctgtcattt
    22261 gcggaagttg aaaaagaggc aattattaac gcagcccagg tcacaggcgg tcgcattcag
    22321 gaaatgtcgg ctttacttgg aatcggccgc actacgctgt ggcggaaaat gaagcaacat
    22381 ggcattgatg cagggcagtt taagcgccgg gtatgaaaga cagaaacgat ttctaataca
    22441 tcagagtgat ctatatttca ttccggcgca cgctaacatt tttcagcatc gtttaagggc
    22501 ttgtctatca cgcacttaaa agctgaagcg atatcctccg ttgacttgct tctgatcaaa
    22561 gaggttaccc tgcgtgtaat ccgcttcgag gtagaatgtg tgctgtttgt tatactgtgc
    22621 actgactccc acgccgttat tccagccatt acctttgaaa ctgtacttct cccgggagtt
    22681 gttcaacaga tattcggtat cccctgaaaa ctcgcggata gcgccagtct tcacatagac
    22741 attcagctgg ctgttgcctg cggtgatgtc ataccccagt atcatgctgg cacgccccag
    22801 cagcgattcg tagtgattca gatgtatatt gaggccatta ctcgccttca tagccatctc
    22861 attctggtgg ctgtatgtaa gctgggtttg cggctctata tagaacccat aaccagtagg
    22921 ggacaggttg aacctctgcc cggcttccag ggagatgctc attccattcg cagtgccgtt
    22981 ggcgttaacg ccgttgttct gactgtccag tacgtggaaa ctatttttct ggcgcgatgc
    23041 ttttataacg agatcgctgt aaaaaccgtt ttgtgccatg taactggcgt acattcccat
    23101 gtagtctgaa cgtgcggtac cgtcgcctcc gctatagtcc ggcgatgcat gtgttgagcc
    23161 aatatacaga ccgacataca acggcatttc atcagagaga cgtttatccc caccaaactg
    23221 gataccgctg taacccatgt caaagccgct cagtttgcca ctggcaaagg agtccaggct
    23281 tcccccataa ctgcgcaacc agatattacc gtctttactc tgatttcgca ggtcacccat
    23341 ccgttgcatc agcgtacggt tttcaacata gttcaataag taaccgacat tgagataatt
    23401 gccgccagca gctgcggtcg tggtgggttt aggcgtggga gcgggttcag gcgtaggatc
    23461 ggggttgact atgggaggct gagcgggagc tggtgtgggt tcaggattag gagtcggttc
    23521 cggaactgtc ccggaagcgt aaagctccca gttagtgcca tttttacgca catcgtacag
    23581 atatcccccc aactcaacct gagaagacgc gctgaacgag gccgcgccgt cagtggtttt
    23641 taccactgtc agaacttcat ttcccgttgt ggcctcgctg ccctggttgc ggatagccaa
    23701 tacgtgatta ccagcactac tcccgctgat attcaataaa tcccctttat tattaacgcc
    23761 attaccctcg ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc
    23821 tacgtttaat gtggcaaatg tgccggcagt tgacccgtgg ctggcaaaat cgacagttga
    23881 atggctcagc gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt
    23941 cattgcaacg tccagttttc cgccattgac attatcgctg agggaggaac ctgtccaaac
    24001 cgacccaggg tgcatatcca gattgattag cccacctttg gaaagaacgc taccgttgat
    24061 aagcatacga cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc
    24121 aatggccatc tggtcgggtg tactcatatc aatgaccaga tcgctcgtga ggtcgatctg
    24181 gctgttggtc atggcataaa ttcccctggc tcctgccgcg ccggtgatag ccaaactgtc
    24241 tccggttatt ctaccgccgc tgagcgccca caaacccagc gccagactgc cattacgatc
    24301 aactgtaata tcggtatttt gcatgttgac aacagccgtt gccgtctggg ccgaggcacc
    24361 ataagaaccg ccggaaaaga tgctgtttcg ttgcgctgcc gtgccagaaa aattgattgt
    24421 cgcgtctgaa ccactggtga cgaggccacc gccctgcgcg gaagaaatat agctatcagc
    24481 accgatagtg gttgtaccac cacgaacttc gacgccattg gccgcggctc cagttacatc
    24541 aacagtgagt gcattcgcgc taacctggcc aaagctccag aggccgtgtg cattatcgcc
    24601 attggtttta atggaactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat
    24661 ccccatggcg ctgtagccct gaacatcgat tgtcaggtct gtagccgtaa aacgcgcagc
    24721 accattggcg ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtcgt
    24781 gattttactt ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat
    24841 accgttcgag ttttcaatgg tgaattgagt ggctgttagt gttgagctat gcccaataat
    24901 gatgccgtca tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc
    24961 accaattaag ttaatgccga tggcagaggt ttgaccaaca acatctactg tgagtcggtt
    25021 agctgtcagg gagctgtttt tgccctgaat aatgattcct tttgcatatc cagaggcgtc
    25081 gttgacattg acggtgacat cattacccag gttgatattg ccaggcgtgt ttcctgctgg
    25141 ggtcattaca ccatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc
    25201 atcattaata gtaatattgg tgccatattg acttcctgta tcagcagcta tcagcagggg
    25261 ggaaaagata attgcattca aaccaatagc gccaaatttt atcagttgaa ttgagggcga
    25321 acggattttt aattcagcga gacgatctgc tttggtattc ccattgtgtt gtttgatgcc
    25381 cataccactc ctatatagta cccagactat gggtctat
//
LOCUS       DAZFAH010000048        25271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFAH010000048 DAZFAH010000000
VERSION     DAZFAH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.048"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1048
                     /locus_tag="ACHJWB_001884"
     CDS             <1..1048
                     /locus_tag="ACHJWB_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4313532.1"
                     /translation="DTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQGKQG
                     APLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRVNMDF
                     TQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHYYSDG
                     AFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKENIAN
                     TGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKPGAKL
                     CIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVTERHD
                     NLGVGHTLLICQKK"
     gene            1089..1811
                     /locus_tag="ACHJWB_001885"
     CDS             1089..1811
                     /locus_tag="ACHJWB_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO4313533.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            1808..2629
                     /locus_tag="ACHJWB_001886"
     CDS             1808..2629
                     /locus_tag="ACHJWB_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO4313534.1"
                     /translation="MSKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            2604..2861
                     /locus_tag="ACHJWB_001887"
     CDS             2604..2861
                     /locus_tag="ACHJWB_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO4313535.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            2873..3124
                     /locus_tag="ACHJWB_001888"
     CDS             2873..3124
                     /locus_tag="ACHJWB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4313536.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3129..3710
                     /locus_tag="ACHJWB_001889"
     CDS             3129..3710
                     /locus_tag="ACHJWB_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313537.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            3707..5065
                     /locus_tag="ACHJWB_001890"
     CDS             3707..5065
                     /locus_tag="ACHJWB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO4313538.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            5052..5405
                     /locus_tag="ACHJWB_001891"
     CDS             5052..5405
                     /locus_tag="ACHJWB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO4313539.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5396..7072
                     /locus_tag="ACHJWB_001892"
     CDS             5396..7072
                     /locus_tag="ACHJWB_001892"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4313540.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLTRLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIEGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7076..7498
                     /locus_tag="ACHJWB_001893"
     CDS             7076..7498
                     /locus_tag="ACHJWB_001893"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO4313541.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7495..8100
                     /locus_tag="ACHJWB_001894"
     CDS             7495..8100
                     /locus_tag="ACHJWB_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO4313542.1"
                     /translation="MKFLPLLALLISPFVSALTRDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8069..10387
                     /locus_tag="ACHJWB_001895"
     CDS             8069..10387
                     /locus_tag="ACHJWB_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO4313543.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIASYYPCGIAWVDRKSSF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10384..10968
                     /locus_tag="ACHJWB_001896"
     CDS             10384..10968
                     /locus_tag="ACHJWB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO4313544.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            10970..12139
                     /locus_tag="ACHJWB_001897"
     CDS             10970..12139
                     /locus_tag="ACHJWB_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO4313545.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFSRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12136..12600
                     /locus_tag="ACHJWB_001898"
     CDS             12136..12600
                     /locus_tag="ACHJWB_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO4313546.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            12600..13331
                     /locus_tag="ACHJWB_001899"
     CDS             12600..13331
                     /locus_tag="ACHJWB_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO4313547.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13328..14557
                     /locus_tag="ACHJWB_001900"
     CDS             13328..14557
                     /locus_tag="ACHJWB_001900"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO4313548.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(14731..15000)
                     /locus_tag="ACHJWB_001901"
                     /pseudo
     CDS             complement(14731..15000)
                     /locus_tag="ACHJWB_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15397..16428)
                     /locus_tag="ACHJWB_001902"
     CDS             complement(15397..16428)
                     /locus_tag="ACHJWB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4313549.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            16699..17142
                     /locus_tag="ACHJWB_001903"
     CDS             16699..17142
                     /locus_tag="ACHJWB_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4313550.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17158..17445
                     /locus_tag="ACHJWB_001904"
     CDS             17158..17445
                     /locus_tag="ACHJWB_001904"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4313551.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17458..18714
                     /locus_tag="ACHJWB_001905"
     CDS             17458..18714
                     /locus_tag="ACHJWB_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4313552.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(18930..19214)
                     /locus_tag="ACHJWB_001906"
     CDS             complement(18930..19214)
                     /locus_tag="ACHJWB_001906"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4313553.1"
                     /translation="MEQKTLSAEPRRLFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(19635..20647)
                     /locus_tag="ACHJWB_001907"
                     /pseudo
     CDS             complement(19635..20647)
                     /locus_tag="ACHJWB_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(20659..21975)
                     /gene="fucP"
                     /locus_tag="ACHJWB_001908"
     CDS             complement(20659..21975)
                     /gene="fucP"
                     /locus_tag="ACHJWB_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4313554.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22003..22923)
                     /gene="rbsK"
                     /locus_tag="ACHJWB_001909"
     CDS             complement(22003..22923)
                     /gene="rbsK"
                     /locus_tag="ACHJWB_001909"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4313555.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            23226..24008
                     /locus_tag="ACHJWB_001910"
     CDS             23226..24008
                     /locus_tag="ACHJWB_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO4313556.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(24010..24236)
                     /locus_tag="ACHJWB_001911"
                     /pseudo
     CDS             complement(24010..24236)
                     /locus_tag="ACHJWB_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(25011..>25271)
                     /locus_tag="ACHJWB_001912"
     CDS             complement(25011..>25271)
                     /locus_tag="ACHJWB_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4313557.1"
                     /translation="TVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT         6151 a         6490 c         6350 g         6280 t
ORIGIN      
        1 ggacacgctc agcgcactgg atgccatcac cgaagcacag cggattgcct ttgctcctat
       61 gcttttccag gcagctttgt gcctgcgcaa tgcaggaata ttagattatt tggatcaaca
      121 gggtaaacag ggagctcccc ttaatgcgat tactgagcac acagtcctga atgaatatgc
      181 cgtgggtgtg ttactggata tgggcttaag cgggcgaatt attacctgta aagagggaat
      241 ttattacctg gcgaaaatcg gtcattacct cctgcatgac actatgactc gcgtgaatat
      301 ggatttcacc caggatgtct gttaccaggg gctcttcttc cttgccgatt cactcaatga
      361 aggaaaacct tccgggttaa aagtatttgg cgactggccg acgatatatc cggcactttc
      421 gcaattacca gacgccgcac gtgatagctg gtttgcgttt gatcattact attcagatgg
      481 cgcatttaac gccgcactgc cttatgtatt cgccaacaac cccacaacct tgtacgatgt
      541 gggtggcaat acgggtaagt gggctttgcg ctgctgcaaa ttcaatgaaa acatcgccgt
      601 tacgttatta gatctgccac aacaaatcgt actggcgaaa gaaaatatcg cgaatacagg
      661 tttttctgat cgcatagatt tccatgcggt ggatatgcta agcgatgccc cattaccagg
      721 cgaggctgat atctggtgga tgagccaatt tttagactgt ttttcacccg aacaaataat
      781 tagcatactc agcaaaattg ccagcgtaat gaagcctggc gcaaaacttt gcatcatgga
      841 gttgttctgg gatgcacagc ggtttgaagc gacatcgttt agcctgaatg catcctcact
      901 ttattttacc tgcatggcaa acggtaacag ccgtttctat agtgcagaga aattttacga
      961 ctatctgaat aaagcaggat tccaggtaac agagcgtcac gataatttag gcgttgggca
     1021 tacacttttg atatgccaaa agaaataatc gtcacttaat aattttggtt taccgcgggc
     1081 gcgcgttgat gaaatttgca ttaaatatta ccaactggca ggcactggcg cctggactta
     1141 gcgatgtgca gcaatggcaa gcatggtcgc gccagccgtg ggcgattgac cctgccgcgc
     1201 cgttggcaaa attaagtgaa ctgccaatga tgaccgctcg tcgtctcagt tcaggcagca
     1261 aactggctgt tgaatgcggt ctgacaatgc tacgccgcca tcagccagac gctgttttgt
     1321 ataccagtcg tcatggcgaa ctggagcgca attaccgcat tgttcatgca ctggcaaccg
     1381 aacaggcgct ttctccgacg gacttcgcgc tttccgtaca caactcttcc gtgggtaacc
     1441 tgaccattgt ggccaaacag ccgatcgttt cgtcatcgct ttcggctggt cgcgacagtt
     1501 tccagcaagg tttgtgtgaa gtgcttagcc tgctacaagc gggataccag cgcgtgttga
     1561 tggtcgattt tgatggcttt ctacctgagt tttatcaccc gcaactgccc gcagaaatgc
     1621 ctacctggcc gtacgccgtc gcgctggtga ttgaagctgg cgatgactgg caatgtgaaa
     1681 cgcagccagc cattgcggta aatgaaacaa cactgccgca aagcatcttg tttttacagc
     1741 actatttaca aaatgcagat gctttttcac tgcctggcga gcgcgtgcaa tggcgctgga
     1801 gccgcagatg agtaagctga tgtcgcgcct gggatgggca tggcgactgg tcatgaccgg
     1861 gttatgtttt gtcctgtttg gtctcggcgg cctgctgctt tcagtcgtgt ggtttaacat
     1921 attgttagtt ttggtgtggg atacttcccg ccgccgtcgt ttggcacgtc gcagcattgc
     1981 cgccagtttt cgcctgtttt taactgtcgc gaaagggctt ggcgtgctgg attatcgtat
     2041 tgatggcgct gaaattttac gtcaggaacg cggttgcctc gtcgtagcca atcatcccac
     2101 gctgatcgat tacgtgctgc tggcatcagt catgccagaa actgactgcc tggtgaaaag
     2161 cgcgctgtta aaaaatcctt ttcttggcgg cgtcgtgcga gcggcagatt atttaatcaa
     2221 cagcgaagca gaaaccctgt taccccgctg ccaacagcga ctggcacagg gtgacactat
     2281 cctgattttc cccgaaggca cacgaacgaa gcccggtgaa aaaatgacgt tacagcgcgg
     2341 tgcagcgaat attgccgtgc gctgcaccag cgatttacgc atcgtgacga tcggctgtag
     2401 cgagcatctg ctggataaac aaagtaagtg gtatgacgtc ccgcctgcca ggccgttttt
     2461 tacggttgaa gtccgtgggc gggtagaaat caataaattt tacgatgcaa cttcacaaga
     2521 gccgacgctg gctgcaagac agttaaaccg gcatcttttg cttcaattac agcaaggttg
     2581 tttacctcta tcaggaataa ataatgcaag cgctttatct ggaaattaaa aacctcatca
     2641 tcagcaccct caatctggac gacttgactc ctgatgatat tgatatcaat gcgccactgt
     2701 ttggcgacgg tttagggctg gactctatcg atgcactgga actgggactg gcagtaaaaa
     2761 atgagtacgg catcgtgctc tctgcggaaa gcgaagaaat gcgtcagcac tttttctccg
     2821 tcgccactct ggcctctttc attgctgcgc aacgtgcctg aggaaaacca tcatgacaga
     2881 ccaacaaacc atttatcagg aagtctcagc cctgctggtt aagctgtttg aaatcgatcc
     2941 gcaggacatc aaacctgaag cgcgactgta cgaagatctg gaactggaca gcatcgacgc
     3001 cgttgacatg attgtgcacc tgcaaaagaa aaccggtaag aaaatcaagc cggaagagtt
     3061 taaagcagtg cgtacagtcc aggatgttgt cgaggctgta gaacgcctgc tacaagaagg
     3121 ctaatttgat gtcgggcatt cgctcgctac cgatgattaa gttgctgacc ggattattgc
     3181 tgctcgtctg gcctttttta atctggctag gtttggtgca taacagcctg cactggctgt
     3241 tgccgctgat ggcgctgttg cttttattgc gcctgcgcca gccccgtcgc cagacggggc
     3301 cgctccaggt cgtcacaaaa atcgttgctg ttgtgggcat tgtattgtgt gtctccagtt
     3361 ttctgttgaa aacgtaccag ttactgctgt tttacccggt agtggttaac gcagtcatgc
     3421 tggcggtttt tggcggttcg ctgcggtcga caatgccgat agttgagcgg ctggcacgtt
     3481 tgcaggagcc agacctcccg gaaaaggccg tgcgatatac ccgccgcgta acgcaggtct
     3541 ggtgcctgtt ttttattttc aatggcagta ttgcgttatt taccaccttg tatggcaaca
     3601 tgcccttgtg gacagcctgg aacggtatca ttgcttacct tctgataggt ttcctgactg
     3661 caggggaatg gctgatacgt cgtaagatga tcaaacggaa aacaccatga atcagactct
     3721 ctcactttca cagtggttga cagcctctcg ccctgtaaca actcctgttg cctggcttgg
     3781 ggaatatacc tggacactgg ggcatctgcg gcatgatgta gcgcttttga tcgaccattt
     3841 acgcgaccag ccaggcaatc gctgggcact ttgttttgaa aacagctatc tttttattgt
     3901 agcgttgttg gccacgctac actcaggtaa aatcccggta atcccgggcc ataatcgcgc
     3961 cgctttgtta aatgtacaac gctccttgtt tgattgcgtc ctgagtgata aaaggctggg
     4021 ctggaacggt ccacagtttg ttgtaagcac atctcaccag atgacgacca gcacgatagc
     4081 ctttgatgat attgcttctg acgcgtttat tgaacttttc acatcgggtt caaccggaca
     4141 accaaaacaa attgcaaaac ctgttatcag ccttgatcaa gaagcaaaca tgctggcaac
     4201 tcactttgca gaccgactac tgggctgtcg ttttgtagca tccgtgatgc ctcagcacct
     4261 gtatgggcta actttccgta tttttttacc catggcactt ggcctgccat tgcatgccgc
     4321 aatgctctgg tattctgagc aacttgccgc attaagccat acctaccgct atgcctttgt
     4381 cagcagcccg gcgttcctta agcgactcga ccttcatctg acgcctcccc cagtggaaat
     4441 gattctctct gccggcggta tgctaccatg gcaggatgta gagaaaactt ccacctggct
     4501 gaatatatgg cccgatgaaa tctatggtag taccgaaaca ggtattctcg catggcgtta
     4561 ccgtcaacag gataatatcc cctggtttac tttttctgat gttcatcttt cacaggaaag
     4621 tgagggggtt cgtgtctttt cgccactaat ccctgctgaa ggattggtgc ttgacgatat
     4681 gttgcaattc aatgaaaacg gtcagttcca cctgatcgga cgacgtggtc gagtggtaaa
     4741 aatagaggag aagcggatat cactggttga aatcgaacaa cgacttctgg cacttgacgg
     4801 tattatggat gtcgctgcaa taccattaat acgcaatggc cgccaggcta tcggtgtcgt
     4861 ggttgtacca aataaagagg cccgtcaaat atggcaatgc tccggaggga aaactctgga
     4921 gttgtcctgg cgcaaagcac tattaccatg gcttgaacct gttgcagtac ctcgttactg
     4981 gcgcattatt gatgaaatac cagtaaacag tatgaacaag cgtgtctatg cgcaattaca
     5041 ggagttattt catgatacgc catgaaattg agcgccatct ggttcaaccg caggagatgg
     5101 aaatcgttct ataccttgac ccgatgctct actggtttaa tggtcatttt gccgtacagc
     5161 ctctgttacc aggcgtggcc cagttggact gggtaatgca ttatgctacc acgctgcttg
     5221 ctccgggctg gcgttttcgt agtatccaga atgttaaatt tctggcgcca cttatacctg
     5281 agacgactgt taccctgcaa ttaacatggc aggaaacaag tcaggtactc actttctgct
     5341 atcagcgtca cgacggtgac gcccgccaca ccgccagtag cgggaagatc cgtctatgtc
     5401 ggtaaacttt tctccctgcg tgttgatccc ctgctacaac cacggcgcga tgatgccggg
     5461 cgtgctgacg cgtcttaagc catttaatct gccctgtatt gtggtggatg acggcagcga
     5521 tgccgccacg caacagcaac tggataatct ggttagcgaa cagcctggcg tgaccttaat
     5581 tcgcctggca gaaaacgcag gtaaaggcgc agcagtgatg cgtggcttgc aggcagcggc
     5641 agacgcaggg ttcagccatg cggtgcaggt ggatgctgac ggtcaacatg cgattgaaga
     5701 tatccccaaa ctgctggcac tcgctgaaca acaccctgtg gcgctgatct ccggtcagcc
     5761 aatttacgat gactccattc cccgctcacg gctgtacggg cgctgggtca cccacgtctg
     5821 ggtatggatc gaaacgctct ccctgcaact gaaagacagc atgtgcggtt ttcgcgttta
     5881 tccggttgcg ccgacgctgc aactggcaaa acgcgccacc atcggcaagc ggatggattt
     5941 cgacaccgaa gtgatggtgc gcctctactg gcagggaaat accagctatt tcgtgccgac
     6001 ccgcgtcacc tatccactgg acgggctttc gcattttgat gccctgaaag ataacgtccg
     6061 catctcgctc atgcacacgc gtctgttttt cggcatgttg ccgcgtattc cttcactgct
     6121 gatgtgccac tcttccagcc actgggcgcg gcagagtgaa gttaaagggt tatggggaat
     6181 gcgcctgatg ctgctggtct ggcgtctgct gggaagaacg gcgtttaccg cgctgcttta
     6241 cccggtggtt ggcgtctact ggctcaccgc tgctcgtgcg cgtaaagcgt cgcaagactg
     6301 gctcgcccgt gtgcgccaac aacaaccaca ggcagcaaaa atcaacagct atcagcactt
     6361 tctacgtttc ggcaacgcca tgctcgacaa aatcgccagc tggcgcggcg agctgcaact
     6421 cgggcgtgat gtgctgtttg cgccaggcgc agaagcggcg cttgacgtcc gcgatccgcg
     6481 cggcaaattg ctgctggcct cacatcttgg cgatgtggaa gtgtgccggg cgctggcaaa
     6541 aattgagggc tacaaaacca ttaacgcgct ggtgtttagc gaaaacgccc aacgctttaa
     6601 acagataatg caggagatgg ctccccaggc aggcattaac ctgatgccgg taacagatat
     6661 cggcccggaa accgccatcc tgctgaaaga gaagctggat aacggcgaat gggtggcgat
     6721 tgtcggtgac cgcatcgccg tcaccccgca acgcggcggc gactggcgcg tctgctggag
     6781 tccgtttatg ggccagcctg cgcccttccc acaggggccg tttattctcg cctctatttt
     6841 gcgctgcccg gtgaatctga ttttcgccct acgccagcac ggcaagctgc atattcactg
     6901 cgaaaccttt gccgacccgc tgctgttgcc gcgtggcgaa cgccaacagg cgctgcaaaa
     6961 cgctatcgac cactacgccg cgcgtctgga acattacgcg ctccagtcgc ccctcgactg
     7021 gtttaatttt ttcgatttct ggcaactgcc ggaaattcag gacaaggagt aaagggtgct
     7081 taacgatccc cgatttaccg ctgaagtcga gctgaccatt ccctttcacg acgtcgatat
     7141 gatgggcgtg gcctggcacg gtaactattt tcgctacttt gaagtggccc gcgaggcgct
     7201 gctcaaccag ttcaattacg gctatcggca gatgaaagag tccggttatt tgtggccagt
     7261 ggtcgatgcg cgggtgaaat atcgccacgc cctcaccttt gagcaacgca tccgcgtgcg
     7321 tgcgcatatc gaagagttcg aaaaccgtct gcggattggc tatcagattt tcgatgccga
     7381 aacgggcaaa cgcgccacca ccggatacac cattcaggtc gcagtcgacg agcaaagccg
     7441 cgaactgtgc tttgtcagcc ccgatattct gtttgaacgc atgggagtca aaccatgaaa
     7501 tttttaccgc tgctggcgct gctgattagc ccgtttgtga gcgccctgac ccgggacgat
     7561 cttcagcaac gctttaccga acaaccggtg atccgcgccc atttcgatca aacccggacg
     7621 attaaagatc tgccgcagcc gctgcgatct cagggtcaga tgttgatcgc ccgcgaccaa
     7681 gggttattgt gggatcaaac ctcaccgttc cccatgcaac tattactgga cgataaacgc
     7741 atggtgcagg tgatcaacgg tcagccgccg caaatcatca cggcagaaaa caacccgcag
     7801 atgttccagt ttaaccacct gctgcgcgcg ctgttccagg ccgatcgcaa agtgctggaa
     7861 caaaacttcc gcgtcgaatt tgccgacaaa ggcgaaggcc gctggacgct gcgcctgacg
     7921 ccgaccacca cgccgctgga caaaattttc aacaccatcg atctcgccgg gcaaacctat
     7981 ctggagagca ttcagcttaa tgataaacag ggcgatcgca ccgatatcgc tcttacccaa
     8041 catcaactga caccagcgca actgaccgat gacgaacgcc aacgctttgc cacccagtaa
     8101 acgccccgcg ctgttatggg ggctagtctg cctggtcatg gcggcggcgt tgctgatcct
     8161 gctgccacaa tcacgactga acagtagcgt gctggctatg ttacccaaac aggcgatggg
     8221 cgatattcct ccggcgctga atgacggctt tatgcagcgt ctggaccgcc aactggtgtg
     8281 gctggtcagc cccggtaaag aggctaatcc cagggtggct caggagtggc tgacgctgct
     8341 gcaaaaatcc gctgcgctcg gcgacattaa agggccaatg gatgccgcca gccagcaggc
     8401 gtggggagcg tttttctggc agcaccgcaa cggcctgatt gaccccgaca cccgcgcccg
     8461 cctgcaaaat ggcggcgaag cgcaggcgaa gtggatcctc tcccagcttt attctgcgtt
     8521 ctccggggta agcggcaagg agctgcaaaa cgatccgctg atgttaatgc gcggctcgca
     8581 gctggcgatg gcgaaaaatg gccagcgttt acgactaatg gacggctggc tggtgacaca
     8641 ggatccccag ggcaattact ggtatctgct gcacggcgaa ctggcgggat cgtcgtttga
     8701 tatgcagcaa acccaccagc tcatcacgac cctgaatacg ctggaaaagg ctctgaaaac
     8761 gcgttacccg caggcgcagt tgctctcacg cggcacggtg ttttacagcg attacgccag
     8821 ccaacaggcg aagcaggata tctccaccct gggcgtggca acgctgctgg gggtgatatt
     8881 gctgattgtg gcggtgttcc gctctttgcg cccgttgctg ctttgcgtga tttccatcgg
     8941 catcggcgcg ctggcgggaa cggccgccac attgttgatt ttcggtgaat tacacctgat
     9001 gacgctggtg atgagcatga gcgttatcgg catttccgct gactacacgc tctattatct
     9061 caccgagcgg atggtgcacg gcaacgacgt ttcgccgtgg caaagcctgg cgaaagtacg
     9121 caatgccctg ctgctggcgc tgctcacaac cgtggcggca tatctgatta tgatgctcgc
     9181 ccccttcccc ggcattcgcc agatggcgat ttttgccgcc gtcgggttga gcgcctcctg
     9241 tctgaccgtc ctgttctggc atccgtggct gtgccgtggt ctgccggtgc gcccggttcc
     9301 ggcgatggcg ctgatgctac gctggctggc agcgtggcgg cgcaataaaa aactgtcgcg
     9361 gggtctgccc gttgcgctgg cgctgttttc gctggcgggg atgtcaatgc tgcgcgtcga
     9421 tgacgatatc tcgcagttac aggcgctgcc gcagcacatt ctggcgcagg agaaagccat
     9481 taccgccctg accggacaaa gcgtcgatca aaaatggttt gtagtttacg gcgactcccc
     9541 acagcaaaca ttgcggcgac tggagaaata taccgcctca cttgagtatg cgaaaaaaga
     9601 ggggcttatc agcaactacc gcactattcc gctgaactcc ctggcgcggc aggaggaaga
     9661 cttagacctg ctgaaaacgg ctgccccgac ggtaacaaaa gcactgcaaa atgcagggct
     9721 gacggcggtg aaaccagatc tcaacgccat gccggtgaag gtcgatgaat ggctggcaag
     9781 ccccgccagt gaaggctggc gtctgctgtg gctgacgctg gaaaacggcg aaagcggtgt
     9841 actggtgccg gttgaagggg ttaaaagtag cgcattgttg caggaaatcg ccagctatta
     9901 cccttgcggc attgcctggg ttgatcgcaa aagctccttt gatgaattgt tcgcacttta
     9961 ccgctacgtc ttaaccggct tgttgctggt ggcgctggca gtgattgcct gcggcgcagt
    10021 ggcccgtctc ggctggcgca aagggcttat cagcctggtg ccttcggtgc tttcgctggg
    10081 ctgtggtctg gcggtgctgg cgatgagcgg gcaggcggtg aatctctttt cgctgctggc
    10141 gctggtgctg gtgcttggca tcggtatcaa ctacacgctg tttttcagta atccgcgcgg
    10201 tacaccgtta acttcgctac tggcgatcgc gctggcaatg ctcaccacct tgctgacgct
    10261 gggaatgttg gtattcagcg ccacccaggc catcagcagt tttggcattg tgctggtgag
    10321 cggtattttc accgccttcc tgctttcgcc gctggctatg cccgataaaa agagaacaaa
    10381 aaaatgatca aatccacatt ctggcgagcg ttcgccctga ccgctacgct tatcctcacc
    10441 ggctgtagcc actcgcaacc agaacaggaa ggccgcccgc aggcgtggct gcaacctggc
    10501 acgcgcatca cgctgccggc accagggatt tcacccgcag tcaattcgca gcaactgttg
    10561 accggcagct tcaacggcaa aacccagtcg ctgttagtga tgcttaacgc cgacgatcag
    10621 aaaatcaccc ttgccgggct gtcgtcggtc ggcattcgcc tgtttctggt gacctacgat
    10681 gcgcaagggc tacgcgccga gcaatccatc gtcgtcccac agttaccgcc cgcaagtcag
    10741 gtactggctg acgtgatgct cagccactgg ccgattagcg cctggcaacc gcaacttccc
    10801 gcaggatgga cacttcgcga caacggtgac aaacgcgagc tgcgtaacgc cagcggcaaa
    10861 ctggtcacgg aaatcaccta tctgaatcgc cagggaaaac gcgtgccaat cagaattgag
    10921 cagcatgtct ttaaatacca catcaccatt caatacttag gtgactgata tgatttatat
    10981 ttccgccgtc ggcatgatca acgccctggg aaataacctt gacgaaatcg cagccaacct
    11041 ggctcgcggt gtcgcccccg gcatgcgccc acgcgcaggc tggttgcagg ggcatccgca
    11101 ggcggtcctg gctggcgtgg atggcgagct tccgcttatc ccggaaaaat tcgctgcgca
    11161 tcgctcgcgc aacaatcaga tcctgctggc ggcgctggcg cagctccagc cgcaggttga
    11221 tgacgctatc gcgaaatatg gctgcgagcg catcgcgatt gtgcttggca ccagcacctc
    11281 cggcctgcat gaaggcgaca cgcacgttaa cctgcacacc cacgggcagc cgagcaccac
    11341 gtggcactat gcacaacaag aactcggcga tccgtcgcgt tttctcagcc actggctggc
    11401 gctcgacggc ccggcttata ccctttcaac cgcctgctcc tccagtgcca gagcaatgat
    11461 cagcgggcgc aggcttatcg aagcggggct ggtcgatgcc gccattgtgg gtggcgcaga
    11521 caccctgagc cgaatgccga ttaacggttt tcacagcctc gaatcgctgt cgccaacctt
    11581 atgtcagccg tttagtcgtg accgcgcagg tatcaccatt ggtgaagggg cggggctaat
    11641 gctgctgacc cgggaaccgc agcccatcgc gttgctgggc gttggtgaat cgagcgacgc
    11701 ttaccatatt tcagccccgc atccgcaggg cgaaggagcg atccgcgcta ttaatcaggc
    11761 gctgaccgat gcgcagctta cgccggatga cgtcggctat atcaacctgc acggcaccgc
    11821 cacacagctc aacgaccaga ttgaatcaat ggtggttaac gccctgtttg gcgaacgcgt
    11881 accgtgcagc tccaccaaac atttgaccgg acacacgctc ggcgcggctg gcattaccga
    11941 ggcggcaatt agtatgttga ttttacagcg tgatttaccg ctgcccgctc aggacttcag
    12001 cctgtcgccg cgcgatccca cgctgccacc gtgtggcatt atcgaaaaac cgcagccgct
    12061 ggcacgtccg gtgatcctgt ccaattcctt cgcctttggc ggcaataacg ccagcattct
    12121 gctcgggagg gtttcgtgag ccactattta tcccccggcg cttatctgcc ccacgacgcg
    12181 cccatgttac tgctggaaga cgtggtgagc gtcagcgatg acagcgcggt gtgccgcgtg
    12241 acggtttcgc ccagtggcgt actggcaccg tttctcgacc cggacggcaa tctccctggc
    12301 tggtttgccc ttgaactgat ggcgcaaacc gtcggcgtct ggtctggctg gcatcgccac
    12361 cagcagggca aaaacagtat tgagttaggg atgatactgg gcgcgcgtga attgctttgc
    12421 accactggca ttctgcccgc aggccagacg ctgaccatta ccgtcaaact gctgatgcag
    12481 gacgatcgct tcggcagctt tgagtgcagc atcaacgacg gcgaggcgac gggccgcatt
    12541 aacaccttcc agccgaccgc ggaagaactt accaccctgt ttcaacaagg agcgtcctga
    12601 tgagtcgttc agttctggtt actggtgcca gcaaaggcat tggtcgcgcc atcgcctgca
    12661 agcttgcggc agacggcttt aacattggcg ttcactatca ccgtgacgcc acaggcgctc
    12721 aggaaacgct gaatgccatt gtcgccaacg ggggtaacgg gcgtttgctc agtttcgacg
    12781 tcgcaaaccg cgaacagtgt cgggaagtgc tggagcatga gatcgcgcaa cacggtgcct
    12841 ggtacggcgt ggtcagtaac gccgggatcg cccgcgatgc cgcttttccg gctttaagcg
    12901 atgacgactg ggatgcggtg atccacacca acctcgacag tttctacaac gtcattcagc
    12961 cgtgcattat gccaatgatc ggcgcgcgcc agggtgggcg catcatcacg ctgtcctcgg
    13021 tttccggcgt aatgggcaat cgcggccagg tgaattacag cgcagccaaa gccggaatta
    13081 tcggcgcaac aaaagcactg gctatcgaac tcgcaaagcg caaaatcacc gtcaattgca
    13141 tcgcgccagg gcttattgat accgggatga tcgaaatgga agaaagcgcc ctgaaagagg
    13201 caatgtcgat gatccccatg aaacgtatgg gccaggcgga agaagttgcc gggcttgcca
    13261 gttatttaat gtctgatatt gcgggttacg tcacccgcca ggttatttcc atcaacggag
    13321 ggatgttatg acacgtcgcg tagtgattac aggaatgggc ggcgtgactg cctttggtga
    13381 aaactggcag gacgtttctg cacgcttact ggcgtatgaa aatgcggtgc gcaaaatgcc
    13441 ggagtggcag gtctacgatg ggctgcacac cctgcttggc gcgcctgttg atgatttcac
    13501 gctaccggag cactacaccc gcaagcgtat ccgtgcgatg ggccgcgtgt cgcaaatgtc
    13561 gacccgtgcc agcgagctgg cgttagagca ggcagggttg attggcgatc cgatattaac
    13621 cagcggcgaa acgggcattg cgtacggttc atcgaccggc agtaccggtc cggtgagcga
    13681 atttgccacc atgctgactg aaaagcacac gaacaacatc accggcacca cctatgtgca
    13741 gatgatgccg cacaccaccg ctgttaatac tggcctcttt ttcggcctgc gcgggcgggt
    13801 gatcccaacc tccagcgcct gtacctccgg cagccaggcg atcggttatg cgtgggaagc
    13861 cattcgccac ggttatcaaa cggtgatggt tgccggtggt gccgaagagt tatgcccttc
    13921 agaagcagcg gtgtttgata ccctttttgc caccagccag cacaatgatg cgccgaaaac
    13981 cacaccttca ccgttcgatg aaaaccgcga cggactggtg attggtgaag gcgcaggtac
    14041 gctcattctc gaagaactgg agcatgccaa agcgcgcggc gcgaccattt acggtgaaat
    14101 cgtcggcttt gcgactaact gcgacgcggc acatattacc cagccgcagc gcgaaaccat
    14161 gcagtattgt atggagcaat cgctgaaaat cgcagggtta agcgcacaag atatcggcta
    14221 tatttcagcg catggtacgg cgaccgatcg cggtgatatg gcagaaagtc tggcgacagc
    14281 aaccatttac ggtgataatg tgccgctctc ctcgctgaaa agttattttg gtcatacgct
    14341 tggtgcctgt ggcgcgctcg aagcctggat gagtctgcaa atgatgcgcg aaggttggtt
    14401 tgcgcctacg ctaaacctga ataaaccaga cccgaattgt ggcgcgctgg attacatcat
    14461 gcatgaagcc cgcaaggttg attgtgagtt tttgcagagt aacaactttg cctttggcgg
    14521 cattaataca tccatcatta ttaagcgctg gccctgagtt atacaaccac atggacatcc
    14581 catgtggtga ttcttcaaaa aatctgaatc aatgaatatg aacctcaaat tctcttacgc
    14641 atactgaaga gcattccgta taacgattca gcagacaaaa ctttatcagt ccacacgaat
    14701 caacggatgc acagatgaca cgcccggtta tcaccgggca atggttgcca taacgggtaa
    14761 cataagggac tgaacagttg tctttagcgc aacgaactga tctaacgtac ctgaacggac
    14821 aacctgaaaa cagccttcca tcctgcattg tccgccagtg cttattacca accacgtctg
    14881 ttgcagtaaa gtaatcagtt gcgaacatta tttatcaatc gtgacgctgg ctcgcgcatg
    14941 gcagaaaagg tcctgactga gcaacaacag gcatcgtact gttgctgctc catgttgttt
    15001 atatgaacgc atcccgtttg caaggcttcc gtgtttttga cgttgacggt agattgctgc
    15061 tctatatcct tattgcagcc ggtgctgcgg gcctcaatga gattcgctga ctctcgcctt
    15121 attcacgcaa cgagctttaa gctcagaaaa acatccaagt ttggtgagtc acagtcttac
    15181 ctgtcgttgt catcaatata ggaatggata atctggagag cgatactgca ggctgatatt
    15241 gcggttttgc gacaagtttt tggcatccgc ttctacgcca aatacctgaa gtaggaatgg
    15301 atgcgttagc agcatacatc cggaactgcg cggggaatag ccggaaacgg ctatccctac
    15361 cacaatagct aactttgtaa tagcgctctt ttaggctcat gtactctgac gtaattctat
    15421 ctggtagttg aattgcacga tacgcgactc ttctgattca tcatgattat tattcaatag
    15481 tccaatcagt aacttacctg tctctatccc catttttttg tagtcaatac ggatggatgt
    15541 caaagttggg tgtgtttgat cgcaattgtc tgaaccgtca agacaagcta cagcgatatt
    15601 tccagggatt tttaacaaac gccgctggca ctcaaacatt atacccagcg caatctcttc
    15661 gtggctgcaa atcacagcat ccagctctgg ttgtcgcagt aatatctcgt ttaatgcgta
    15721 acgccccaat tgcagacttg cggcatccgg catggtgata acaagatcag tgttctggta
    15781 atggctcagc attgctttat gccagccgtt cagttgctga cgttgcaggc ggttgtccat
    15841 atgcgcaccg ataaatccga tattcttata accctgctcc agcagatgtt cggtcagttt
    15901 atgtgatgct tcaaaaaatg ctgtttcgat ggtaatttga gccggactga aaggggaacc
    15961 gataacgtta atggtaggta tatttgtttt ctcgagtatc tggtgagttt tatcagccaa
    16021 ctgagaacca aaaataacta acgccgccgg gttgccttga agaagcgtca tcagaatttc
    16081 agcttctttg agctgattat actcatgaca cccaagcagt agctggaatt catttttatt
    16141 aagcacctgc tgtaatgact gcataaagcg ggagctggct ttatctgtca gggaaggaac
    16201 cagtacggcg actgtatgac tgtgaccaga tgcaagggcg ccagcagttc tgttgggaat
    16261 ataacccaac tcttctacgg cctgttctat cttttccctc agcttgtcag aaacctgttc
    16321 cggtgttctc agcgcgcggg aaacggtcat ggttccgaca cctgcaaagt tagcgacctc
    16381 ctgcagcgtc acccgtccgg taccacggcg cttacgtgtt ttttccattt cgactccatg
    16441 gataattaca accagctgtt ttcattactt ttatttcacc attactcttt ccagtttttg
    16501 cccacggtag caaatatcct gttgcattgt atatgcagga accgaatata gccaaagcat
    16561 ggcggcagag aaatgatagc gctatcacaa agattggtag cgctatcaga tagcgctatc
    16621 aaatgcgaca atcatcacat taattaatgc gtaaaatatc tactctcctg ttaattatca
    16681 tcgggagtta tcagtagcat gttaaaaaat ctattaaata cagaggtcgt tcaggtcgtg
    16741 gaacaggtca aagactggcg ggaagccgtg gcgatttcat gccgaccgtt gattgaaaac
    16801 ggctctatcg aaccgcgtta tgttgatgcg atctatcact cgcacgacac tatcggtccg
    16861 tactatgtgg ttggccctgg tattgcgatg cctcatgcgc gcccggaaga gggggctaac
    16921 aagctgtcat tagcactgac cctgatccct tcaggtgtga attttgatgc cgatgaaaac
    16981 gatccagtga aattactgat tgtgctggct gcaaccgaca gtaccagcca cattgaagcg
    17041 atttctcaac tcgccaagtt attcgataac gaaaaagaca tacaggccat tttgacggcc
    17101 aaaacgacgc aggacatttt gtctgttatt gcgcgctatt aatccagagg aacatttatg
    17161 aaaattacag tggtttgcgg taacggctta ggcaccagcc tgatgatgga aatgagcatt
    17221 aaaagcattt tgaaggattt gtccgtcagc gctgatgttg atcacgtcga cctgggctcc
    17281 gcgaaaggga cgccgagtga tatctatgtc ggcaccaaag acattgccga gcagttagcc
    17341 gcgcagtctg tcgcaggcaa aatcgtcgca ctggaaaata tgattgataa gaaagccatg
    17401 agagaacgtt tgtccgtggt attaaccgaa ttaggcgcac tgtaagctgg gggcgatatg
    17461 gatttctttc gttttttgat gagcgatgta ctttccgaac ccgcagtgct ggtcggttta
    17521 atcgccctga ttggcctgat tgcacagaaa aaaccggtaa ccgaatgcat taaaggtacc
    17581 gtcaagacca ttatggggtt tgtgatttta ggcgcagggg cgggtttagt tgtgtcgtca
    17641 ctgggtgact tcgcaaacat tttccagcat gcctttggta ttcagggtgt agtgccgaac
    17701 aatgaagcca ttgtttccgt agcgcagaaa agcttcggga aagaaatggc gatgattatg
    17761 ttctttgcga tggttatcaa cattatgatt gcccgtttca caccgtggaa atttatcttc
    17821 ctgaccggtc atcacacgtt gtttatgtcg atgatggtag cggtaattct ggcaacagca
    17881 ggcatgaccg gcattacgct tatagccgtt ggctctttgg ttgttggcgt ggcaatggtc
    17941 tttttcccgg ctattgcgca tccgtacatg aagaaagtga ccggttctga tgacgttgcc
    18001 atcggccact tctcaacatt atcttatgta ttggccggtt tcatcggcag caagtttggt
    18061 aataaagagc actcgactga agacatgaat gtgccgaaaa gtctgctgtt cttgcgcgac
    18121 acgccggttg ctatctcttt taccatgagc ataattttcc tggtgacgtg cctgtttgcg
    18181 ggtgcagatg cggtgaaaga gcttagcggt ggtaaaaact ggttcatgtt ctctatcatg
    18241 caatccatta ccttcgcagc tggcgtgtac atcattctgc agggtgtgcg catggtgatt
    18301 gcggagattg tcccggcatt caaaggtatt tcagacaagc tggttccgaa tgccagacct
    18361 gctctcgatt gcccggtcgt cttcccttac gcacctaacg ctgtactggt tggtttcctg
    18421 agtagctttg ctgcaggttt aatcggtatg ttcacgttgt atctgctgaa catgatcgtg
    18481 attattcctg gtgtggtgcc gcacttcttc gtaggtgcag ctgcaggggt attcggtaat
    18541 gcaacgggtg gacgtcgtgg tgcgattttg ggtgccttcg ctcagggctt gcttattacc
    18601 ttcctgccag tattcttatt gccggtgctg ggtgatattg gttttgctaa caccaccttc
    18661 agtgatgctg actttggcgc actgggtatt ttgttaggga ttatcgttcg ctaatatttt
    18721 gatgacatta atatcctcca ggatttcagg aacgtggagg atattaattc ctcttatttt
    18781 ctccattttt ctttcttcca gtcaggactg catccgatta atcgtaagcg ctccgagaag
    18841 tacgtagcgt aaggattatt ttacagctga gaagtttcag tgcagcagtt catgcacacg
    18901 gttcgacggc tagtcattga tcttcataat tacctcgcgt aaaaaacggg gtaaggccgc
    18961 tcatccagcg tcatctccac aggtaataat tcaacgccaa tgccagaaga gttcgttacc
    19021 ggaagacgcc gtgatatacg ccctttgatc agcgagagcc tgagccattt gaacagaaga
    19081 ttatcattga tatcatgctc ccgggcaata cgggcaacag aaccccttgg ttgtaaagcc
    19141 agtttaacca tttgaagttt aaactcattt gaaaataacc tgcggggttc agctgataat
    19201 gttttttgtt ccataacagg tgtccactag ttgaaaaggt gggtacctat gttaccaata
    19261 ctggcttaat ggctacatac agcagtcggt ttacgcatac acttttatag tgcactccgg
    19321 cctcatgaat ataacggtgg attaccgccg aacgaatcgg aaaatagata ctggaaaaaa
    19381 actgaaattg tgaccagttt ttgttgacca ctacaaataa ataaaagagt tgcagtgctt
    19441 gctcggtaag aaaacaatgg aggttaagct cctcaaagaa gctgtggagt acaccactct
    19501 acagtagttg cgcaaatttg gagtggacga taatgtgatc gtctataagg gcaacgctat
    19561 catagtcttg tcctggcggg taaaaaacgc gcttacctta acgataagcg cgccgctgtt
    19621 caggccttga gtggttattc aattcctgtg gtgactgtaa aagtgcgcgt ttgctgcggt
    19681 gcaacctgaa tcagcgtgcc attacgttgc gcggcaagat acccctcagg ccgacaggtt
    19741 gcaggtaatg caaaggcggc tacctgttgc tctccgttat aaaggatcca gcgtgtcaca
    19801 taatttagtt cagcactgta gaaacgagta acaaacgtag tgccatcggg agagatcatg
    19861 cgaaactctg gctgatctgt ataagcgtcc agtttgtctg caaagaagac aatttctgga
    19921 tcataaaatt ccggttgact agcgtcgaca gagaggcttc tccctgcata atccgttgat
    19981 taaacgccag ccactgagcg gtgggattaa catgcgaagg cactgattca cgcaatctta
    20041 atatttcgtc cgggatattc tggctgaatg tagcatttgg tatatatgca taattcatgt
    20101 ggcacatata ttgtagtggc atatctacag aagccagatt ggttacggcc atcttaatat
    20161 cgaacagtgt agaggatttg tgaaggacca ctgttggctg agccagataa tgatgaccga
    20221 aacccattac atactcgtaa cgcccgttaa ggcgtaacat atctccgtct aattccagcc
    20281 atgcttcatc catcgcggca caggccattt caccgtgtag cagatgagta tcttccgcag
    20341 atgggcagcc attagccagc aaacctgaat gaaaagcaaa acagccatag gtctctatca
    20401 cctctgtcgc cggtttaggc tggcgaaaca tattgcacat ggtgaggccg tgtccatcaa
    20461 attgcgcatc ccaaatcatc agccccatcc agggaagaat aatcaaatgt ccacgactgt
    20521 ttgcaatttt aagcccctcg acaccgctgt catatcgaaa agacgtgaca gtaaaatcac
    20581 tattttccag caagatacga ggtttctcgc caaaaagcgc ccgccacaaa ttaatacgcg
    20641 tactcataac ggttctcctc aggatgctgt gacttcagcc agtgcggtac gtactttgct
    20701 ttcacgccag aagtagactc cgacatagac aaagcagagc atagaaacca ggaatgaaag
    20761 ctgtagtgag tggaacatat ctgcaatata tccctgaatt gccggaacca ccgcggcacc
    20821 gacaatagcc ataacaatga ctgctcctgc catttctgta tgttcgttat caactgtatc
    20881 cagtgttcct gcatagatcg tcgcccagca agggccaaac aaaacactta ccaggacggc
    20941 gacatagacc gcgctgaaac ttggagccag tgcaacatat gccaggaaca gcgcccctat
    21001 aacggaatag agaatcaata ctttttccgg attaaaacgc gtcataagga tattggctat
    21061 aaacttgcca ataaagaagc aggcaaagct atagaccatg aagtttgaag catcacgttc
    21121 gttgatatcg cccaactcca gcgccagacg gatggtaaat gaccatactg cgacctgcat
    21181 acccacataa aggaactgcg ccacaatacc gcgacgaaag cgcggatttc tagccagata
    21241 gcgcagcgta tccattgctg acgggcgttt atggtgactt gtctgtgcca ctttacaggt
    21301 tgggaagcgg gttaaaagga acaacaccat gaccacaacc agaatcataa tcatatactt
    21361 atacggttca agggtgttct ctaacatcag caccttaaag ttgtgaattt gctcggcgtt
    21421 cattcctgac atctgctttt caaggctttc cccctcggag aaaaccagat atttgcccaa
    21481 taaaatacca gacgcagcac caatcggata aaaggtctgg ctgatattga gccgcaatgt
    21541 ggcataggct tttggaccga tcattgaact gtatgtgttc gctgcagttt caaggaaact
    21601 caggccaatc gcaatcgcaa aaatagctgc aaggaacata gtgtaggttg ccatatgcga
    21661 ggcagggaaa aaaagtgtac aaccaccaat atacagcgtc aggccaatta aaattgccac
    21721 cttataactg gtctttttaa tcacaaggga tgctggtatt gcaattaaaa aataacctcc
    21781 ataaaatgcg ctctgcacca atgctgaagc aaagttactt agcgaaaata cacttttgaa
    21841 ttgagtgatt aatatgtcat ttaatgcagc tgcgcatccc catagcggga ataaacacga
    21901 taacaaaata aactggaaca agggagtctt attcagatac ccatcaggca tctgaatgat
    21961 gtttttatcg ttcatagtgc tacctttaac tgtgcaggat gattattcgt ttaaggttaa
    22021 aaattcatta aattgttcaa tactcggata agatgattgc gtacctttcc ctgtgacgct
    22081 gaaagcggca aagagagcgg cttttttcaa agcggcttca acatcaccgc tttgaacata
    22141 ataatgggaa aagcaaccaa taaatgcgtc accagcgcca ctagtatcaa cagcatttac
    22201 tttgaatgca ggaacatgga cttcctgatc gcgggtcatc cataatgcgc ctttttcgct
    22261 catggtaaca ataatattgt tcagcccttt atcaactaac gaacgtgcgg ccaaacgaat
    22321 atgatcataa gtatcaaccg acataccggt taatatttcc agttctgttt catttgggat
    22381 aaagaaatca catttgcagg cataagacat atctaactca cgcaatgccg gagccggatt
    22441 taataacact tcaataccat ttttcttacc aaactcaatc gcgtggtaaa ctgtttccag
    22501 ttgaacttcc agttgtaaaa cgatcaattt gcattttttc agatcttctg cagctcgatc
    22561 gatatcttcc ggggaaagaa atttattcgc tcccttaatt attaatatac tattgctcga
    22621 gttggcatta acaaagatcg gtgcaacacc actgctggta caggggactt tctcaacata
    22681 agtggtatta attccccatg attcgagatt acgaatagta ttatccgcaa aaatatcatc
    22741 acctacttta gtcagcatca ggacttttga attcaattta gccgccgcca ccgcttgatt
    22801 agcacctttc ccaccacatc cgattttgaa ggcaggtgct tccagagttt ctccttcttt
    22861 aggcatctga ttagtgtaag taatgagatc caccatattg gaaccaataa ctgcaatgtc
    22921 catttcacta cctcttataa actttcgcat aacaatggta tttaaataac attagcatgt
    22981 tacttttgca tcatttgtga ctgagatcgc gattagcaca tcaacccgat gtttatttaa
    23041 tagacttcca gtcttatcac tcaggctaac actatctaat cataagcaac ctaacaggat
    23101 taataccgaa aattcagcag tctataccct tttcatttca aagggtcggt cgtatagtat
    23161 ggtaactaaa acaatgttta ctaatgccat aatgttattt ttataacatt ttacggagag
    23221 agttgatgga aacgaagcaa aaagagcgta tccgacgttt gattgaaata cttaagaaaa
    23281 ccgacagaat ccatttgaaa gacgcggcac gaatgctgga agtttctgta atgactattc
    23341 gtcgcgatct ccatcaggaa gatgaacctc tgccactgac cctactgggt ggctatattg
    23401 taatggtgca taaacccgca ccatccatgc cagtaatcca ggacgttccg agaaatcatc
    23461 gtgatgactt acctattgca attctggccg ccggaatggt taatgaaaat gatctgatct
    23521 tctttgataa tggccaggag ataccgctcg ttataagcat gatcccggat gcaatcacct
    23581 tcactggcat ctgttactca catcgtgtct ttgttgcgtt gaatgaaaaa cctaatgtga
    23641 cagcaatact ttgtggtggt acgtatcgtg ccagaagtga tgctttttac gatgccagta
    23701 actcttcgcc attagactct ctcaatccgc gaaaaatatt tatttccgcc agcggtgtac
    23761 atgatcactt tggcgtcagc tggtttaatc ccgaagatct tgccactaag cgtaaagcga
    23821 tggcccgtgg actaaggaaa attttgctcg cccgccacgc cttgttcgat gaagtagcct
    23881 ctgccagcct cgcaccgctc tctgcatttg atgttctgat tagcgagcgt ccgttaccgg
    23941 cagattatgt tacgcactgc cggaatgctt ctgtaaagat cattacacct gattcagaag
    24001 acgaatgact tactgaaaaa acaccgtact cttgttaaac atcgtcggat tggactgatt
    24061 acgttgcact ttcatcacat attccagttt atcaatttgg cttatcatct actccagacg
    24121 ctggtcatca ttgaccaata gtctgatatg gcctttgtcg ctgtcctgaa tcggcaggca
    24181 gaaaatgcct tcaacgttaa aagcacgacg ggaaaaagcc cacaaaggtt agtcattacg
    24241 cccggataat aaacaggctc ctccctgtta tggaagccgt tgtattgtcc ataatgtctg
    24301 ggtttttaat gcacactgga taacaaccgg acgaatcttc aaatattact ggcgctgaca
    24361 gccggtttga attcatctca caaccctgca tagagcgaat ctcctgcctg cacgtcactc
    24421 cactccatgg tatcaacttc acactcttta tctgcggcta gtttcagtca ccagataagc
    24481 atcgactatg aaaggtgagc catgacaaca taacgttggt aacgctctga cgccttaacg
    24541 gaagatgcct gccaccacag ggaatgtaaa cgactgaagt gtggccttta atgccgtgaa
    24601 cggctcatgg tctcctggca cggttgccgc cccaacctgt aacaacattc cacagtacaa
    24661 tgtctgtcag agccggagcc tcccatgcct gttgaataaa accccggatg cttcccccga
    24721 ttctgcaagt aatacttcgg ttatttacac tattttatcc ctgttgaatt tggttaagcc
    24781 ggtgcagacg cgccgggaaa cgcacgcctg aaccggcaca ttaaaataca atacaacccc
    24841 ctgttactgg agatacgaat ggaatgacga ggcagcctgt cagtcacagg agggctgttg
    24901 gctcaaaatc aacggaaagg ccaccaacac cggaattaag ctccacagtc cccgcactgg
    24961 caatccattc ccgggaacgg cattaccgca aactgctccg gaggcaacca ttacagcctc
    25021 tgtaacggat tatcagccct ggcatcttcc gacggcacct cctgccgccc ccacgccctg
    25081 ctgtataccc cggtaccgca gcgtgggcag cacttttccc cctcataatc ttcgtcacac
    25141 atcacacaaa accactttct caccttcacc gtataatccg gaaccgtcat gccctgcaga
    25201 tgcaaacgac gggcctcttt ctctgccggg tcaagccagg tacatcgcag caggattttt
    25261 tcagcgacag t
//
LOCUS       DAZFAH010000049        24801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFAH010000049 DAZFAH010000000
VERSION     DAZFAH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.049"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..741
                     /locus_tag="ACHJWB_001913"
     CDS             <1..741
                     /locus_tag="ACHJWB_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO4313558.1"
                     /translation="QPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRL
                     PRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAG
                     QVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCF
                     KLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            897..2432
                     /gene="gadC"
                     /locus_tag="ACHJWB_001914"
     CDS             897..2432
                     /gene="gadC"
                     /locus_tag="ACHJWB_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4313559.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2563..3882
                     /locus_tag="ACHJWB_001915"
     CDS             2563..3882
                     /locus_tag="ACHJWB_001915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4313560.1"
                     /translation="MDICSRNEKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQRAVSYLQSRWGS"
     gene            4254..5636
                     /gene="dosC"
                     /locus_tag="ACHJWB_001916"
     CDS             4254..5636
                     /gene="dosC"
                     /locus_tag="ACHJWB_001916"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4313561.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5661..8060
                     /gene="dosP"
                     /locus_tag="ACHJWB_001917"
                     /gene_synonym="pdeO"
     CDS             5661..8060
                     /gene="dosP"
                     /locus_tag="ACHJWB_001917"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO4313562.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTTRDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICCNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLIDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8318..8899
                     /gene="ddpX"
                     /locus_tag="ACHJWB_001918"
     CDS             8318..8899
                     /gene="ddpX"
                     /locus_tag="ACHJWB_001918"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4313563.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8913..10463
                     /locus_tag="ACHJWB_001919"
     CDS             8913..10463
                     /locus_tag="ACHJWB_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4313564.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10465..11487
                     /locus_tag="ACHJWB_001920"
     CDS             10465..11487
                     /locus_tag="ACHJWB_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4313565.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLNDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11484..12380
                     /gene="ddpC"
                     /locus_tag="ACHJWB_001921"
     CDS             11484..12380
                     /gene="ddpC"
                     /locus_tag="ACHJWB_001921"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4313566.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12377..13363
                     /locus_tag="ACHJWB_001922"
     CDS             12377..13363
                     /locus_tag="ACHJWB_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4313567.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13356..14282
                     /locus_tag="ACHJWB_001923"
     CDS             13356..14282
                     /locus_tag="ACHJWB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4313568.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14338..14769)
                     /gene="osmC"
                     /locus_tag="ACHJWB_001924"
     CDS             complement(14338..14769)
                     /gene="osmC"
                     /locus_tag="ACHJWB_001924"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4313569.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15114..15329
                     /gene="bdm"
                     /locus_tag="ACHJWB_001925"
     CDS             15114..15329
                     /gene="bdm"
                     /locus_tag="ACHJWB_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4313570.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15431..15568
                     /gene="sra"
                     /locus_tag="ACHJWB_001926"
     CDS             15431..15568
                     /gene="sra"
                     /locus_tag="ACHJWB_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4313571.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15725..17422
                     /gene="maeA"
                     /locus_tag="ACHJWB_001927"
     CDS             15725..17422
                     /gene="maeA"
                     /locus_tag="ACHJWB_001927"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4313572.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17556..18566
                     /gene="adhP"
                     /locus_tag="ACHJWB_001928"
     CDS             17556..18566
                     /gene="adhP"
                     /locus_tag="ACHJWB_001928"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4313573.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMVCCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHSAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18712..18996
                     /locus_tag="ACHJWB_001929"
     CDS             18712..18996
                     /locus_tag="ACHJWB_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4313574.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19047..19700)
                     /gene="fdnI"
                     /locus_tag="ACHJWB_001930"
     CDS             complement(19047..19700)
                     /gene="fdnI"
                     /locus_tag="ACHJWB_001930"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4313575.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19693..20577)
                     /gene="fdnH"
                     /locus_tag="ACHJWB_001931"
     CDS             complement(19693..20577)
                     /gene="fdnH"
                     /locus_tag="ACHJWB_001931"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4313576.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20590..23637)
                     /gene="fdnG"
                     /locus_tag="ACHJWB_001932"
     CDS             complement(20590..23637)
                     /gene="fdnG"
                     /locus_tag="ACHJWB_001932"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23050..23052),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4313577.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVESLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23869..24750
                     /gene="yddG"
                     /locus_tag="ACHJWB_001933"
     CDS             23869..24750
                     /gene="yddG"
                     /locus_tag="ACHJWB_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4313578.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6035 a         6319 c         6299 g         6148 t
ORIGIN      
        1 caaccgctgc acgatgcgct ggataaattc caggccgaca ccggtatcga catcgacatg
       61 cacatcgacg ccgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      121 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      181 ccgctgggct gcggctgggt tatctggcgt gatgaagaag cgctgccgca ggaactggtg
      241 ttcaacgttg actacctcgg tggtcagatt gggactttcg ccatcaactt ctcccgcccg
      301 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctatacc
      361 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      421 gggccgtatg agttcatctg taccggtcgc ccggacgaag gcatcccggc ggtttgcttc
      481 aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga acgtctgcgt
      541 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      601 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
      661 tacaaagcct ccctgaaata tctcagcgac cacccgaaac tgcaaggtat tgcccagcag
      721 aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt
      781 tcgggacaat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt gttctgaata
      841 cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg gataatatgg
      901 ctacatcagt acagacaggt aaagctaagc agctcacatt actcggattc tttgccataa
      961 cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg ggcttttcat
     1021 tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga ctttgtgctg
     1081 cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata
     1141 ctctggggcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa atcgccattg
     1201 gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg aaatggccag
     1261 cgctgaatga agaccctatt accaaaacta ttgcagcact catcattctt tgggcgctgg
     1321 cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg
     1381 ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttat ctgcactccg
     1441 gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc tctaaagtgg
     1501 gcaccctggt tgtatttgtt gccttcattt tgagttatat gggcgttgaa gcttccgcaa
     1561 cccacgtcaa tgaaatgagc aatccagggc gcgactatcc actggctatg ttactgctga
     1621 tggtggcggc aatctgctta agctctgttg gcggtttgtc tattgcgatg gtcattccgg
     1681 gtaatgaaat caacctctcc gcaggtgtaa tgcaaacctt taccgttctg atgtcccatg
     1741 tggcaccgga aattgagtgg acggttcgcg tgatctccgc actgctgtta ctgggtgttc
     1801 tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc acagcgcaga
     1861 aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg
     1921 tcatttcgca gctggtgatt acttctatcg cgttgatcat cctcaccaat accggtggcg
     1981 gtaacaacat gtccttcctg atcgcactgg cgctgacggt ggtgatttat ctgtgtgctt
     2041 atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac ttaaaacgca
     2101 catttaatat cccaggtggt aaaggggtga aactggtcgt ggctgttgtc ggtctgctga
     2161 cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag ggtgactcta
     2221 ccgatatgta tgttgaatta ctggtcgtta gtttcctggt ggtacttgcc ctgcccttta
     2281 ttctctatgc tgttcatgat cgtaaaggca aagcgaatac cggcgtcact ctggagccaa
     2341 tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca cgttcaccac
     2401 actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat aagataaggg
     2461 agcgataatt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa ccgggataag
     2521 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
     2581 aacgagaaat taacgattag aagaccagcg atactggttg cactggcact tttactgtgt
     2641 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
     2701 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtttctcgc
     2761 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
     2821 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggtat aaacacggtc
     2881 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
     2941 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
     3001 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
     3061 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
     3121 agcgtctatg tgcaacaccg tgactggatc agaacgtctg gcgatcgctt tgtcctcgac
     3181 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgcagaagt ggtttcccgc
     3241 tatccggtag atggcgtgca gtttgacgac tatttctata cggagtcacc gggttcacgg
     3301 ctaaatgata acgaaacgta ccgcaaatac ggaggcgcat ttgcgtcaaa agcagactgg
     3361 cggcgcaaca atactcagca gttaattgca aaagtatcgc acaccattaa aagcattaag
     3421 ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
     3481 ctcggttccg atacccgagg tgcggcagcc tatgacgaat cctacgctga cacccgtcga
     3541 tgggtggaac aaggattgct ggattatatt gctccccaaa tttactggcc gttctcacgg
     3601 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     3661 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa gatagagcca
     3721 gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg
     3781 gtacccgaaa ttagcggcac aatcttgttc cgtgaggact atctgaataa accgcagact
     3841 caacgagcgg tcagctatct gcaaagtcgt tggggcagtt aaactcccat tcttactttg
     3901 taacaagccg gtacattacc caccggcttg ttaccctctt gaactatccc actactaatc
     3961 atgcttacta aagtcaaatt aaccatgcaa ttaaccacac ttagttaaac agccataaat
     4021 cccctaataa aaatgtatga atatgaaaaa acatacgata ttcgctgtca ccgaaaaata
     4081 attacgatgg gctatactct tctgaagaac agtagcgtag attgaaactg tcctaagtcg
     4141 cttatttctt ttcagtatat cttcatattt caggagaata tggtgcagtt agtcgatctc
     4201 gcacgttgcg ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga
     4261 tgtattttaa aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatccgccca
     4321 ttcgtgctaa agccgcggaa attgccgttg cgcatgctca ttatctgagt attgagtttt
     4381 atcgaattgt ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc
     4441 ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg
     4501 atgtcgaaag gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa
     4561 ttccggtaga aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa
     4621 tcttctcttc ggattattcc gccgcagaaa aacttcaggt ctaccatttt tcgattaaca
     4681 gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct
     4741 caaaggaaga tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag
     4801 aacggcaaat agcctcaata ctttcatggg aaatagatat tatctataaa atcctgctgg
     4861 attctgattt aggcagtagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc
     4921 ataaaggtcg acattatttt agtggcattg ccgaagtagg ccatatctcc cgtctgattc
     4981 aggatttcga cggtattttc aatcaaacca tgcgtaacac caggaatttg aataacagaa
     5041 gtctgcgggt gaaattttta ttacagataa gaaataccgt atcgcaaatt attaccttgc
     5101 tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat
     5161 tacttaaccg ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga
     5221 ccggtacacc gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata
     5281 cgtggggcca taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca
     5341 acgtccgcag tagtgattat gttttccgct acgggggcga tgaatttatc attgttttga
     5401 ctgaagcttc tgaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga
     5461 aaaccaaact gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg
     5521 ccatgtttaa tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt
     5581 atatcgccaa aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc
     5641 gccaggatgc agaggtaatc atgaagctaa ccgatgcgga taatgccgcc gatggcattt
     5701 ttttccccgc ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aatgatgaag
     5761 tgatgttttt caaccccgcc gcagagaagc tatgggggta caaacgtgaa gaagtcattg
     5821 gcaataacat tgatatgctg attccgcgag atttgcgtcc tgcgcatcct gaatacattc
     5881 gtcataaccg tgaaggcggt aaagcgcgcg tcgaggggat gagtcgggag ctgcagctgg
     5941 agaaaaaaga cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg
     6001 ggaaagttta ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac
     6061 agacccgaca attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc
     6121 cggaacgcca tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta
     6181 gcgaagccag cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata
     6241 accgcattcg tttacaacag ttgctatgga aaaccacccg cgatcaggac gaatttctgc
     6301 tgttgacgcg caccggtgaa aaaatctgga ttaaagcttc tatcagcccg gtttatgacg
     6361 tgctcgcgca tctgcagaac ctggtaatga ccttctcgga tatcaccgaa gaacggcaaa
     6421 tccgccagct tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa
     6481 tgggggaaat catttgttgt aacatcgaat ctgttctcaa cgaatcgcat gtttcgctgt
     6541 tcgcactgcg caacgggatg ccgatacact gggcgtcatc ttcgcacggt gcagaagttc
     6601 aaaatgcgca aagctggtca gcgaccattc gtcagcgtga tggtgcgcct gcggggatcc
     6661 tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata
     6721 tcagccagca tatggccgcg ctggcgctgg aacaggaaaa aagccgtcag catattgaac
     6781 aactcatcca atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc
     6841 tcgatgacct tatcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata
     6901 ttcaggatgt gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg
     6961 tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc
     7021 agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat tacccaaatc gccgatgagc
     7081 tacggaatgt ggtcagcaag ccgataatga ttgacgataa acccttcccg cttaccttga
     7141 gtattggcat cagctacgac gtgggtaaaa accgcgatta cttgctctcc actgctcaca
     7201 atgcaatgga ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga
     7261 tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca
     7321 ataaccaact gaaactggtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg
     7381 gcatcgaagc ccttgctcgc tggcacgatc ccctgcatgg tcatgtgccc ccttcacggt
     7441 ttattcctct cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg
     7501 aagcttgccg tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg
     7561 tgaacttgtc ggcgctgcac tttcgcagta atcaactgcc taatcaggtg tctgatgcaa
     7621 tgcacgcctg gggtattgac ggccaccagc tgacggtaga aatcacggaa agcatgatga
     7681 tggaacacga taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggct
     7741 tatcggtaga tgattttggt acgggctttt ccggattatc ccgcttagtc agtcttccgg
     7801 taacggaaat caaaattgac aaaagttttg tcgatcgttg tctgaccgaa aaacgcatcc
     7861 ttgccttact tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg
     7921 aaggcgtcga aaccaaagag caatttgaga tgctacgcaa gatccactgt cgcgttattc
     7981 agggatattt cttttcccgc cccctacccg ccgaagaaat tccaggctgg atgagcagcg
     8041 tgttaccgct gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcatttaacg
     8101 tgacactgtc acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac
     8161 ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat
     8221 ataactttca gccagcattc tggcgcttat cccggcatgg catgagatct gcataagcgg
     8281 aaagcgcagc aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt
     8341 tgatttagcc gtaatcttcc ctgatctgga gatcgaattg aaatacgcct gcgctgataa
     8401 catcacaggt aaagctattt atcagcaagc gcgttgtctg ttacacaagg atgcgattac
     8461 cgcgctggcg aaaagtatca gcatcgccca gctgtcaggc ttacaactgg tgatttacga
     8521 tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata
     8581 tgttgttgat gtgacggtcg gttctaatca cagccgtggc acggcgatcg acctgacgct
     8641 tcgtgatgag cacgggaaca tcctcgatat gggcgcaggg ttcgatgaaa tgcacgagcg
     8701 ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag cgcaatcggc tgttgctgaa
     8761 tgcgataatg actggtggcg gttttgtcgg tatctccagc gaatggtggc acttcgaatt
     8821 accccaggca gcgagttacc ctctgctcgc tgatcaattc tcttgtttta tatcccctgg
     8881 cacacagcac gtcagttaat caggaaactg tcatgaagag atcgatatcg tttcgtccca
     8941 cattgctcgc gctcgtcctt gccacaaatt tcccggttgc gcacgccgcc gtaccgaaag
     9001 atatgctggt gattggtaag gccgccgatc cacaaaccct cgacccggcg gtaacaatag
     9061 ataataacga ctggacagtg acctacccgt cttatcagcg gctggttcag tacaaaacgg
     9121 acggtgataa aggctcaacc gacgttgaag gcgatctggc aagtagctgg aaagcgtctg
     9181 acgatcaaaa agagtggacg ttcaccctga aagataatgc taaatttgcc gatggcacac
     9241 ctgtcactgc cgaagcagta aaactttctt ttgagcggct actaaaaatc ggccaggggc
     9301 cagcagaagc atttcccaaa gatttaaaga ttgatgctcc cgacgaacat acggtgaagt
     9361 ttacccttag ccaaccattc gcaccgttcc tctacacgct ggcgaatgac ggtgcatcca
     9421 ttatcaatcc ggcggtctta aaggaacatg cagcggatga tgctcgcggc ttcctcgcgc
     9481 aaaataccgc cggttccgga ccatttatgc tgaaaagctg gcaaaaaggt cagcaattag
     9541 ttctggtgcc aaatccgcat tacccaggca ataaaccgaa cttcaaacgg gtatcggtaa
     9601 aaattattgg tgaaagtgcc tcccgtcgcc tgcagctctc ccgtggcgac attgacattg
     9661 ccgatgcgct gccggtggat caactcaacg ccctgaagca ggaaaataaa gtcaatgtgg
     9721 cagagtatcc gtcactgcgc gttacctatc tgtatctgaa taacagcaaa gcgcctctta
     9781 atcaggcgga tctgcgtcgg gccatttcct ggtctaccga ttatcagggc atggttaacg
     9841 gcattctgag tggtaacgga aaacagatgc gcggcccgat tccggaaggc atgtggggct
     9901 acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa agccaaagcc gaatgggata
     9961 aagtgacgag caaacccacc agcctgacgt ttctctactc cgataacgat ccgaactggg
    10021 agcctattgc tctggcgaca caatccagtc tcaacaagct gggcatcaat gtgaagctgg
    10081 aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa aggtgattac gacattgcga
    10141 ttggcaactg gagtccggat tttgccgacc cgtatatgtt tatgaattac tggtttgagt
    10201 cagacaaaaa aggtctgccg ggtaaccgct cgttctatga aaacagtgag gtcgataagt
    10261 tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg tacccgggac taccagcagg
    10321 cacagaaaat cgtcattgat gacgctgctt atgtgtacct gttccagaaa aactaccaac
    10381 tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc catgctggaa caggtcttca
    10441 atatcaatac catgagtaaa taacgtgacc ttctggagta ttttacgcca acgctgctgg
    10501 gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt ttattatctc gcatctgatc
    10561 cctggtgatc cggcgcggtt actggcgggt gaccgcgcca gcgatgctat cgtggaaaat
    10621 attcgccagc aactgggact ggaccagcca ctgtatgtac agttttaccg ctacgtcagc
    10681 gacctgtttc atggtgacct gggaacatcc attcgtaccg ggcgtccggt gctggaagag
    10741 ttgcgtatat ttttcccggc gacgctggaa ctggcttttt gtgccctgct gctggcactc
    10801 ctgattggca tcccgctggg catactctct gcagtctggc gaaatcgctg gctggatcat
    10861 ctggtgcgaa taatggccat taccggaatc tccacacctg cgttctggct tggactgggc
    10921 gtcattgtgc tgttttatgg tcatctgcaa attcttcccg gcggcggaag gcttaatgac
    10981 tggctggatc caccaacgca cgttaccggc ttttatctgc tcgatgcgct gcttgaaggc
    11041 aacggtgaag tcttcttcaa tgcgttgcaa catctcatct taccggcatt aacgctggcg
    11101 ttcgttcacc tgggaattgt cgctcgccag atccgctcag cgatgctgga acaattgagt
    11161 gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg gctggtatat cgttttatgt
    11221 tatgcgctac ccaatgcgtt gatcccatcg attaccgtat tgggtttggc gctgggcgat
    11281 ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct ggcccggaat gggtgcatgg
    11341 gtagtaacat caatacaggc gctcgacttc ccggcagtga tgggctttgc cgtcgtggtt
    11401 tcatttgctt atgtgctggt caacctggtg gtggatttgc tctatttgtg gattgatccg
    11461 cgtatcggac gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac
    11521 aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca
    11581 ccgtgaccgg cgcagtcatc attgtattaa tgctattgat gatgattttt tcaccgtggc
    11641 ttgcgacgca tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg
    11701 cgcactggtt tggcaccgat gaagtgggac gcgatctgtt cagccgcgta ctggtcggca
    11761 gtcagcaatc aattctcgcc ggattagtcg tggttgccat tgcgggtatg attggttcgc
    11821 tactcggatg tctatccggt gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca
    11881 tggacattat gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg
    11941 ggccgagttt atttaacgcc atgctggcga ttgctattgt gcgaattccc ttttatgtgc
    12001 gcctggcgcg ggggcaagca ttagttgtac gccagtatac ctatgttcag gcggcgaaaa
    12061 cctttggtgc gtcgcgttgg catctgatca gctggcatat tttacgtaac tccctgccgc
    12121 cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg
    12181 gatttattgg cctgggtgct caacaaccga gtgctgaatg gggcgcgatg gtagcaaatg
    12241 gtcgcaacta tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc
    12301 ttaccgccgt tgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag
    12361 caggaggaaa gcagtcatga cccaacccgt tctggacatt caacagctgc atttgagttt
    12421 ccccggtttt aacggtgatg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg
    12481 tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat
    12541 tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg gggcagattt cactgctggg
    12601 agacgatgtt cttaacgctc gggaaaaaca gcttcgtcaa tggcgcggcg cacgagtggc
    12661 gatgatcttt caggaaccga tgaccgccct caatccgaca cttcgaatag gtcttcagat
    12721 gatggacgtg atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat
    12781 tgccctgctg gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt
    12841 tgagctttca ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc
    12901 gcaattgatt attgccgacg aaccgactac ggcgctggac gtcacggtgc agttgcaggt
    12961 actgcgtttg cttaaacata aagcccgcgc cagtggaact gcggttctgt tcatcagcca
    13021 tgatatggcc gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt
    13081 aattgaaagc ggcgtgacgg cagatgttat ccatcatccc cggcatccgt ataccattgg
    13141 tttgctccaa tgcgcaccag aacatggagt accacgccag ctattacccg ccattccagg
    13201 gacggtacca aacctcaccc atttgcctga aggctgcgct tttcgcgatc gttgctatgc
    13261 ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagcg
    13321 ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc tgacacgtta ttaacgttac
    13381 gcgacgtcca tatcaatttc ccggcccgta aaaactggct tggtaaaact acggaacatg
    13441 ttcatgccat taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg
    13501 gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt aatgggcatg ctgcaaccga
    13561 gccacgggca gtacatccgt tctggctcac aacgcattat gcagatggtg tttcaggacc
    13621 cgctctcttc gctcaatccg cgcttaccgg tgtggcgcat catcacagaa ccgctctgga
    13681 tagctaagcg tagtagtgaa caacagcggc gagcgttggc agaggagctg gctgtgcagg
    13741 tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc
    13801 aacgtatcgc cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc
    13861 caacctctgc gctggatatc tccgtgcagg cgcagatcct caatttactg gtaacgctac
    13921 aggaaaatca cgggctgacc tatgtgctga tttcacacaa tgtctcggtg atacgtcata
    13981 tgagcgatcg ggtggcggtg atgtatctcg ggcagattgt agaactgggc gacgcgcaac
    14041 aggtgctcac ggctccggca catccataca cccgattatt gctggattcc ctccccgcca
    14101 ttgataaacc gctggaggaa gaatgggcat tacgtaaaac ggatctgccg ggaaaccgca
    14161 cgttgccgca aggctgtttt ttctacgaac gttgccccct tgcaaccagc ggatgtgaag
    14221 tccggcaatc attagcaata agggaggacg gacgtgagct ccggtgctgg cgggcgctgt
    14281 agagatgcta acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta
    14341 cgatttcaac tggtaatcca gcgtaatttc cgctttcagt acctgagaga ccgggcatcc
    14401 tgcttttgct ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac
    14461 ttcactcttc agtgcgattt tcgtaattgc aaaaccggca tccactttat ccagcgacac
    14521 atcggcggtg gtatcaatcg atgttggcgt gaatcccgct tcccccagca ttaatgaaag
    14581 cgccattgag aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tggttccttt
    14641 ttcgccttca aaacgcgtgt taaatccata cggctgttgg ttcagcacgc cactctcggt
    14701 ggatactgtt cccttcccgc gtttaatatc gccttcccag tgtgcctgac ctttcttatg
    14761 gattgtcatt gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca
    14821 gcacgaggat aagcaggata ttccgaataa aatacggcga aaatattact tctgcactta
    14881 aattaaattc ccacaataga taaccgggaa tatatatctc aagagatatt gcatcaatat
    14941 caatcaactc accacaataa taatacatat caatatcatg gagttaaaat ataaaaatca
    15001 tcaaccagga ctaatcttaa caacgaagcg gcaaatattt gccatgctga atgtgcttta
    15061 taaatctgcg atccgtagca gacaccataa atacacagac acggagaatc actatgttta
    15121 cttattatca ggcagaaaat tcaacagcag aacccgctct ggttaatgcc attgagcaag
    15181 gccttcgggc agagcaaggt gttgtcactg aagatgacat tctcatggag ctgaccaaat
    15241 gggtggaagc ttctgataac gacatcctca gtgatatcta ccagcaaacg atcaattatg
    15301 tggtcagtgg ccagcaccct acgctttaag gtgctatgct tgatcggcaa cctaatttag
    15361 gggtttagca cgtgtttctt cgctacggcg atgttgtcct taaaactagc aacaggattg
    15421 aggagttaaa atgaaatcga accgtcaggc acgtcatatt cttggactgg accataaaat
    15481 ttcgaaccag cgcaaaatag ttaccgaagg tgacaaatcc agcgtagtaa ataacccaac
    15541 cggcagaaaa cgccccgctg aaaagtaatt cataaccatc agtcctcaat gacgattaaa
    15601 caccattgcc tgcgcaatgg tgtttttgtt tttatctgct ttatacttgg ggccgacgcc
    15661 ctggcggtaa agcaaagacg ataaaagccc cccagggatg gatattcaaa aaagagtgag
    15721 tgacatggaa ccaaaaacaa aaaaacagcg ttcgctttat atcccttacg ctggccctgt
    15781 actgctggaa tttccgttgt tgaataaagg cagtgccttc agcatggaag aacgccgtaa
    15841 cttcaatctg ctggggttac tgccggaagt ggtcgaaacc atcgaagaac aagcggaacg
    15901 agcatggatc cagtatcagg gattcaaaac cgaaatcgac aaacacatct acctgcgtaa
    15961 catccaggac accaacgaaa ccctcttcta ccgtctggta aacaatcatc ttgatgagat
    16021 gatgcctgtt atttataccc caaccgtcgg cgcagcctgt gagcgttttt ctgagatcta
    16081 ccgccgttca cgcggcgtgt ttatctctta ccagaaccgg cacaatatgg acgatattct
    16141 gcaaaacgtg ccgaaccata atattaaagt gattgtggtg actgacggtg aacgtattct
    16201 ggggcttggt gaccagggca tcggcgggat gggcattccg atcggtaaac tgtcgctcta
    16261 taccgcctgt ggcggcatca gcccggcgta tacccttccg gtggtgctgg atgtcggaac
    16321 gaacaaccaa cagctgctta acgatccgct gtatatgggc tggcgtaatc cgcgtatcac
    16381 tgacgatgaa tactatgaat tcgttgatga atttatccag gctgtgaaac aacgctggcc
    16441 ggacgtgctg ttgcagtttg aagactttgc tcaaaaaaat gcgatgccgt tacttaaccg
    16501 ctatcgcaat gaaatttgtt cttttaacga tgacattcag ggcactgcgg cagtaacagt
    16561 cggcacactg atcgcagcca gccgcgcggc aggtggtcag ttaagcgaga aaaaaatcgt
    16621 cttccttggc gcaggttcag cgggatgcgg cattgccgaa atgatcatcg cccagaccca
    16681 gcgcgaagga ttaagcgagg aagcagcgcg gcagaaagtc tttatggtcg atcgctttgg
    16741 cctgctgacg gacaagatgc cgaacctgct acctttccag accaaactgg tgcagaagcg
    16801 cgaaaacctc agtgactggg ataccgacag cgatgtgttg tcactgctgg atgtggtgcg
    16861 caatgtaaaa ccagatattc tgattggcgt ctcaggacag accgggctgt ttacggaaga
    16921 gatcattcgt gagatgcata aacactgtcc gcgtccgatc gtgatgccgc tgtctaaccc
    16981 gacgtcacgc gtggaagcca caccgcagga cattatcgcc tggaccgaag gtaacgcgct
    17041 ggtcgccact ggcagcccgt ttaatccagt ggtatggaaa gataaaatct accctatcgc
    17101 ccagtgtaac aacgccttta ttttcccggg catcggcctg ggggttattg cttccggcgc
    17161 gtcacgtatc accgatgaga tgctgatgtc ggccagtgaa acgcttgctc agtattcgcc
    17221 gctggtcctg aacggcgaag gtctggtact accggaactg aaagatattc agaaagtctc
    17281 ccgcgcaatt gcgtttgcgg ttggcaaaat ggcgcagcag caaggcgtgg cggtgaaaac
    17341 gtctgccgaa gctttgcaac aagccattga cgataatttc tggcaagccg aataccgcga
    17401 ctaccgccgt acctccatct aagcctgcac ccggtagtga aggctaccgg gcaattttcc
    17461 tctccctttt tcagaactca tccatactgg gtagtggcga ataaatctca tttgcctcac
    17521 ctgctatgca gaacatcatc cgaaaaggag gaactatgaa ggctgcagtt gttacgaagg
    17581 atcatcatgt tgacgttacg gataaaacac tgcgctcact gaaacatggc gaagccctgc
    17641 tgaaaatggt gtgttgtggt gtatgtcata ccgatcttca tgtcaagaat ggcgattttg
    17701 gtgacaaaac tggggtaatt ctgggccatg aaggcatcgg tgtggtggca gaagtgggtc
    17761 caggtgtcac ctcattaaaa ccaggcgatc gtgccagcgt ggcgtggttc tacgaaggat
    17821 gcggtcattg cgaatactgt aacagtggta acgaaacgct ctgccgttca gttaaaaatg
    17881 ccggatacag cgttgatggc gggatggcgg aagagtgcat cgtggtcgcc gattacgcgg
    17941 taaaagtgcc agatggtctg gactcggcgg cggccagcag cattacctgt gcgggggtca
    18001 ccacctacaa agccgttaag ctgtcaaaaa ttcgtcccgg gcagtggatt gctatctacg
    18061 gtcttggcgg tctgggtaac ctcgccctgc aatacgcaaa gaatgtcttt aacgccaaag
    18121 tgatcgccat tgatgtcaat gatgagcagt taaaactggc aaccgaaatg ggtgcagatt
    18181 tagcgattaa ctcacgcacc gaagacgccg ccaaaattgt gcaggagaaa accggtggcg
    18241 ctcactctgc ggtggtaaca gcagtagcta aagctgcgtt taactcggca gttgatgctg
    18301 tccgtgcagg cggtcgtgtt gtggctgtcg gtctgccgcc ggagtctatg agcctggata
    18361 tcccacgtct tgtgctggat ggcattgaag tggtcggttc gctggtcggc acgcgccagg
    18421 atctaaccga agccttccag tttgccgccg aaggcaaagt ggtgccgaaa gtcgccctgc
    18481 gtccgttagc ggacatcaac accatcttta ccgagatgga agaaggcaaa atccgtggcc
    18541 gcatggtgat tgatttccgt cactaagagg cctttgctgc gactgccatg ttcgggtcgc
    18601 agcatcgcac actctccaac atgaaatggc taaatggatt attgttgcgt aggaaggaac
    18661 aataatgata gtgttcccag tgaaggaacg ctactgagag taaagacact gatgaaaatg
    18721 gccaatcatc cccgcccggg ggacattatt caggaatcac tggacgaact taatgtcagc
    18781 ctgcgcgagt ttgccagagc aatggaaatt gcgccctcaa cggcaagccg attactgacc
    18841 ggaaaagcag ctttgacacc agaaatggcg attaaacttt ccgtggtgat cggcagttcg
    18901 ccgcaaatgt ggctgaatct gcaaaacgcc tggagtctgg cagaggctga aaaaacggtg
    18961 gatgtgtcgc gactgcgccg tctggttacg caataaaaaa atggcgcttc ttaaagcgcc
    19021 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
    19081 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
    19141 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
    19201 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
    19261 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
    19321 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
    19381 ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt caacagccac
    19441 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
    19501 gcgacgaaaa tcgcaatgcc gaaaaacggg tgcaaaatgc gtcccatctg cggcgtaccg
    19561 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
    19621 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
    19681 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
    19741 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
    19801 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
    19861 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
    19921 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
    19981 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
    20041 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
    20101 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
    20161 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
    20221 aatgattgca ccagcagacg ggcacgcctt caggcagccg ggatcttcac agtgcataca
    20281 gccgtcttta cggatcagcc actccagctt gccgttctgt tcggtttcgc taaagcgcat
    20341 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
    20401 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
    20461 acaggtggaa acgtcgataa gttttgcgac ttctgctttg taatcacgca cctgagaagg
    20521 cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg
    20581 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
    20641 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
    20701 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccat ttacattcag
    20761 cggtttcaga cgacgcgtta ccacagccac cgcgcggata aagccacgct tgcttgagac
    20821 agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac
    20881 aaactgttcc ggctgagcaa ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg
    20941 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
    21001 tgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
    21061 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
    21121 gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt
    21181 acccggtgcg gcattgccga agtcaggaat atcgttaccc gtccacttgc tgccgttcca
    21241 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
    21301 gtacagcacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
    21361 ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt
    21421 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
    21481 caactgacct ttcttcgcaa taagcacgcc attagcgtca tagagatctt ccagcgcgta
    21541 gccgttgttc tctttagcca cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
    21601 gctcatcttc atcagcgatt ctacgccttt accaccttcg gcctggtaca gctcgcgcag
    21661 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
    21721 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cttcttcggc
    21781 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
    21841 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
    21901 catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac
    21961 cgggttaaag ccctggcaga aataaccggt gactttgcct tcgtccatca tgttgaaata
    22021 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
    22081 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
    22141 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
    22201 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
    22261 cagacctaag tcagtcagac cctgaatgtt ggagtgacca cgcaatgcat tcacgccgcc
    22321 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
    22381 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
    22441 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
    22501 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
    22561 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
    22621 gttccaggac gatttatcgt attgccgttt ttcagcgtcg tagccgctga acagaccgtc
    22681 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc
    22741 ggcgttgatt ttgtcgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
    22801 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgagg
    22861 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
    22921 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
    22981 atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
    23041 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
    23101 tttctgtgtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
    23161 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
    23221 agctttcatc agctttgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
    23281 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc
    23341 cagcaaaccg gccccttttg ggcacagcgc accacggctt accggatgat ccgggtcacc
    23401 ttcaatgtga taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa
    23461 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
    23521 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
    23581 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
    23641 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
    23701 tttgcgcgaa atcaaaaaga acatctgttt gccacaaaat aatccgcctg cgcaaattga
    23761 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
    23821 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
    23881 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
    23941 cgcggtgtca gcgaggggct cggcccggtt ggcggcgcag ctgctatcta ttcattaagc
    24001 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
    24061 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
    24121 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
    24181 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
    24241 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
    24301 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
    24361 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
    24421 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
    24481 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
    24541 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
    24601 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
    24661 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
    24721 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
    24781 aatcagataa attccgacaa t
//
LOCUS       DAZFAH010000050        24632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFAH010000050 DAZFAH010000000
VERSION     DAZFAH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.050"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            344..1483
                     /gene="wza"
                     /locus_tag="ACHJWB_001934"
     CDS             344..1483
                     /gene="wza"
                     /locus_tag="ACHJWB_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4313579.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1489..1932
                     /gene="wzb"
                     /locus_tag="ACHJWB_001935"
     CDS             1489..1932
                     /gene="wzb"
                     /locus_tag="ACHJWB_001935"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4313580.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1935..4097
                     /gene="wzc"
                     /locus_tag="ACHJWB_001936"
     CDS             1935..4097
                     /gene="wzc"
                     /locus_tag="ACHJWB_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4313581.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLTKEGVTLMVEAIHASRGSEFTITKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4275..5114
                     /gene="wcaA"
                     /locus_tag="ACHJWB_001937"
     CDS             4275..5114
                     /gene="wcaA"
                     /locus_tag="ACHJWB_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4313582.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5117..5605
                     /gene="wcaB"
                     /locus_tag="ACHJWB_001938"
     CDS             5117..5605
                     /gene="wcaB"
                     /locus_tag="ACHJWB_001938"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4313583.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     AENMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5602..6819
                     /gene="wcaC"
                     /locus_tag="ACHJWB_001939"
     CDS             5602..6819
                     /gene="wcaC"
                     /locus_tag="ACHJWB_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4313584.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLINYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6794..8011
                     /gene="wcaD"
                     /locus_tag="ACHJWB_001940"
     CDS             6794..8011
                     /gene="wcaD"
                     /locus_tag="ACHJWB_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4313585.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8023..8769
                     /gene="wcaE"
                     /locus_tag="ACHJWB_001941"
     CDS             8023..8769
                     /gene="wcaE"
                     /locus_tag="ACHJWB_001941"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4313586.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFISEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8785..9333
                     /gene="wcaF"
                     /locus_tag="ACHJWB_001942"
     CDS             8785..9333
                     /gene="wcaF"
                     /locus_tag="ACHJWB_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4313587.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETK"
     gene            9360..10481
                     /gene="gmd"
                     /locus_tag="ACHJWB_001943"
     CDS             9360..10481
                     /gene="gmd"
                     /locus_tag="ACHJWB_001943"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4313588.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10484..11449
                     /gene="fcl"
                     /locus_tag="ACHJWB_001944"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10484..11449
                     /gene="fcl"
                     /locus_tag="ACHJWB_001944"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4313589.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNESYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11452..11931
                     /gene="gmm"
                     /locus_tag="ACHJWB_001945"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11452..11931
                     /gene="gmm"
                     /locus_tag="ACHJWB_001945"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4313590.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11928..13151
                     /gene="wcaI"
                     /locus_tag="ACHJWB_001946"
     CDS             11928..13151
                     /gene="wcaI"
                     /locus_tag="ACHJWB_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4313591.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13154..14590
                     /gene="cpsB"
                     /locus_tag="ACHJWB_001947"
     CDS             13154..14590
                     /gene="cpsB"
                     /locus_tag="ACHJWB_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4313592.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14783..16153
                     /gene="cpsG"
                     /locus_tag="ACHJWB_001948"
                     /gene_synonym="manB"
     CDS             14783..16153
                     /gene="cpsG"
                     /locus_tag="ACHJWB_001948"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4313593.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16208..17602
                     /gene="wcaJ"
                     /locus_tag="ACHJWB_001949"
     CDS             16208..17602
                     /gene="wcaJ"
                     /locus_tag="ACHJWB_001949"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4313594.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17604..19082
                     /gene="wzxC"
                     /locus_tag="ACHJWB_001950"
     CDS             17604..19082
                     /gene="wzxC"
                     /locus_tag="ACHJWB_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4313595.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19358..20638
                     /gene="wcaK"
                     /locus_tag="ACHJWB_001951"
     CDS             19358..20638
                     /gene="wcaK"
                     /locus_tag="ACHJWB_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4313596.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCNALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20635..21855
                     /gene="wcaL"
                     /locus_tag="ACHJWB_001952"
     CDS             20635..21855
                     /gene="wcaL"
                     /locus_tag="ACHJWB_001952"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4313597.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21866..23260
                     /gene="wcaM"
                     /locus_tag="ACHJWB_001953"
     CDS             21866..23260
                     /gene="wcaM"
                     /locus_tag="ACHJWB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4313598.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHVV
                     NVEKINFRLPERRE"
     gene            23435..24328
                     /gene="galF"
                     /locus_tag="ACHJWB_001954"
     CDS             23435..24328
                     /gene="galF"
                     /locus_tag="ACHJWB_001954"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4313599.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELEHTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6028 a         6162 c         6720 g         5722 t
ORIGIN      
        1 cctgcattat tggagagcca atattcaatt accataatgt caccttatat tcggaagtta
       61 atttccgcaa taaccctttc tcatcaacga ttgtatgcag agagggtttg aaaaattaag
      121 ctaaatcagt gtattggtag ctaaaaagcc aggggcggta gcgtgtctgg atgcctgaaa
      181 gaccagtcta aattattctg tcaatagcct gcggatgaaa tcaatttttt taggactgat
      241 gccagttaaa tttgtattca attaactgca aggtaaatgt gcatggcgac attgctatta
      301 atagtgcaca ggataattac tctgccaaag tgataaataa acaatgatga aatccaaaat
      361 gaaattgatg ccattattgg tgtcagtaac cttgataagc ggttgcacag tacttccggg
      421 cagcaatatg tcgacgatgg gcaaagacgt catcaaacag caggacgctg atttcgatct
      481 cgacaaaatg gtgaatgttt atccgctgac cccgcgcctg attgaccaat tacgcccacg
      541 cccgaatgta gcgcgcccca atatgacgct ggaaagtgag atcgcgaatt accagtatcg
      601 cgtcgggccg ggcgacgttc tgaatgtcac cgtctgggat cacccggaac tcaccacgcc
      661 agccggtcag taccgcagct ccagcgacac cggcaactgg gtacagcctg acggcactat
      721 gttttacccg tatatcggca aggtccacgt agtcgggaaa acgctcgctg aaatccgcag
      781 tgatattacc gggcgcttag cgacgtacat cgctgacccg caggtggatg ttaatatcgc
      841 cgccttccgc tcgcaaaagg cctatatctc aggtcaggtg aataaatccg gtcaacaggc
      901 gatcaccaac gtaccactga ccattctcga cgccatcaac gccgcaggtg gcctgaccga
      961 caccgctgac tggcgcaacg tggtgctaac acacaatggt cgtgaagaac gcatttcttt
     1021 gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga ctgctttacc ccggcgatat
     1081 tctctacgtg ccgcgtaatg atgatctgaa agtgtttgtg atgggtgaag tgaagaaaca
     1141 gagcaccctg aaaatggact ttagcggcat gaccctgact gaagccctgg gcaatgccga
     1201 aggtatcgac atgaccacct ccaacgccag cggcatcttt gtcattcgtc cgctgaaagg
     1261 cgagggcggg cgtaacggca agattgccaa tatctaccag ctggatatgt ccgatgcgac
     1321 gtcgctggtg atggcgacag aattccgcct gcaaccttat gacgtggtgt atgtcaccac
     1381 cgccccggtt tcccgctgga accgtctgat caatcagttg ctgccaacta ttagcggtgt
     1441 ccgttacatg acggatacag ccagcgacat tcataactgg taatcgtcat gtttaacaac
     1501 atattagttg tctgtgtcgg caatatttgc cgttccccga cggcggaacg cttactgcaa
     1561 cgttatcacc cggagctgaa agtggagtcc gctggactcg gcgcgctggt cggtaagggc
     1621 gctgatccta ccgctatcag cgtcgccgca gaacatcaac tgtctctgga aggtcactgt
     1681 gcacggcaaa tcagccgccg cctgtgtcgc aactatgacc tgattttgac catggaaaag
     1741 cgccatatcg aacgcttatg cgagatggca cctgagatgc gcggcaaagt gatgctgttt
     1801 ggtcactggg ataacgaatg tgaaatcccc gatccgtatc gcaaaagccg ggaaacgttt
     1861 gcagcggtgt acacattact tgaacggtct gcccgccagt gggcgcaggc attgaacgca
     1921 gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc cgctccggta acgggcagtg
     1981 atgaaatcga tattggtcgc ctggtcggca ccgtcattga agcgcgctgg tgggtgattg
     2041 gcatcaccgc tgtatttgcc ctctgtgctg tggtttacac tttcttcgcc acgccgattt
     2101 atagtgccga cgcactggta caaatcgaac aaaacagcgg caattcgtta gtgcaggaca
     2161 ttggctcggc attagccaac aaaccgcctg catcggacgc cgagatccag ttgattcgtt
     2221 cgcgtctggt gcttggtaaa acggtggacg atctagacct cgatattgcg gtgagcaaaa
     2281 acacgttccc gattttcggt gcgggctggg atcgcctgat gggacgtcag aacgaaacgg
     2341 tgaaagtgac cacctttaac cgtccgaaag agatggcgga tcaggtgttt acgcttaatg
     2401 tgctggacga taaaaactac actctgagca gcgatggcgg ctttagcgcc cgtgggcaag
     2461 cgggccagat gctgacaaaa gaaggcgtca cgctgatggt tgaagctatt cacgctagcc
     2521 ggggcagtga gtttaccatc accaaatact ccacgctggg gatgatcaac caactgcaaa
     2581 acagcctgac ggtaacggag aacggcaaag acgcaggcgt actgagcctg acttataccg
     2641 gtgaagatcg cgaacagatc cgcgacattc ttaacagcat cgcccgtaac tatcaggaac
     2701 aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct cgctttcctc gcgcaacagt
     2761 taccggaagt acgtagccgc ctcgatgttg ccgaaaacaa actgaatgcc ttccgtcagg
     2821 ataaagattc tgttgatctg ccgcttgaag cgaaagcggt gctcgattcg atggtgaaca
     2881 tcgacgcgca gttgaacgaa ctgaccttta aagaggcgga aatctccaaa ctgtacacca
     2941 aagttcaccc ggcgtaccgc acgctgctgg agaaacgtca ggcgctggaa gaagagaaag
     3001 ccaaacttaa cggtcgcgta acggcgatgc cgaaaaccca gcaggaaatt gtccgtctga
     3061 cccgcgatgt cgagtctggt cagcaggtct atatgcaact gctgaataaa gagcaggagc
     3121 tgaaaatcac cgaagccagc accgtcggcg atgtgcgcat tgttgacccg gcaatcactc
     3181 agcctggtgt gctaaaaccg aagaaagggc tgattatcct cggcgcgatt atccttggcc
     3241 tgatgctctc tatcgtgggt gtgctgctgc gctcgttgtt taatcgcggc atcgaaagcc
     3301 cgcaagtgct ggaagaacac ggaatcagcg tctatgccag catcccgctg tcggagtggc
     3361 agaaagcgcg cgatagcgtc aaaaccatca aaggggttaa acgctataaa cagagccagc
     3421 tattggcagt ggggaatcca accgatctgg cgattgaagc catccgtagt ctgcgtacca
     3481 gtctgcactt cgcgatgatg caggcgcaga acaatgtgtt gatgatgacc ggggttagcc
     3541 cgtcaattgg taaaaccttt gtctgcgcca acctggcggc ggtgatcagc cagaccaata
     3601 aacgggtgtt gttgatcgac tgtgatatgc gcaaaggcta cacccacgag ctgttgggca
     3661 ccaataacgt caatggtctg tcggaaattc tgattggtca gggcgatatt actaccgctg
     3721 ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg cggtcaggtg ccgccaaatc
     3781 cttctgagct gttgatgagc gaacgctttg ccgaactggt gaactgggcg agtaaaaact
     3841 atgacctggt gttgattgat acgccgccga ttctggcagt gaccgatgcc gcaattgttg
     3901 gtcgtcatgt cggaaccacg ttaatggtgg cgcgttatgc ggtcaacaca ttgaaagaag
     3961 tggaaaccag tctgagccgc tttgagcaaa acggtattcc ggtgaaaggg gtgattctga
     4021 actccatctt ccgccgcgcc agcgcgtatc aggattatgg ctattacgaa tacgaatata
     4081 agtcggatgc gaaataacga catccgctgt cggatgcgat gctgacgcat cttatccgac
     4141 ctaccttcac gccgcatccg gcaagcaaac cagctccgta ggccggataa ggcgctcacg
     4201 ccgcatccgg caacagaacc cgtaaacaag cttataaacc ggcagaacat ctcgacactc
     4261 cgggagaaca acctatgaaa gacaatccgc tgatttcaat ctatatgccg acctggaacc
     4321 gccaacaact ggcgattcgg gcgataaaat cggtcctgcg ccaggactac agcaactggg
     4381 agatgatcat cgtggatgat tgctccacct cctgggaaca actgcaacag tacgtcactg
     4441 ccctcaacga tccgcgtatt acctacattc acaacgacat taacagcggg gcgtgcgcgg
     4501 tacgtaacca ggcgattatg ctggcgcaag gggaatacat taccgggatc gatgacgatg
     4561 atgaatggac gcccaaccgc ctgagcgtct tcctcgccca taaacagcaa ctggtcactc
     4621 acgccttttt gtacgctaac gactacgtat gccagggaga agtttattcc caaccggcaa
     4681 gcttaccact gtatcccaaa tcgccgtact cacgccgcct gttctataaa cgcaatatca
     4741 ttggcaatca ggtttttacc tgggcgtggc gtttcaaaga gtgcttgttc gataccgaac
     4801 tgaaagcagc acaggattac gacatcttcc tgcggatggt ggtggagtat ggcgaaccgt
     4861 ggaaagtgga agaggcgacg cagatcctgc atatcaatca cggtgaaatg cagatcacct
     4921 cgtcaccgaa aaaattctcc gggtacttcc acttttaccg caagcacaaa gataaatttg
     4981 accgtgcaag caaaaaatat cagctgttta ccctctacca gatccgcaat aaacgtatga
     5041 cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg caaacgtctg gctgatggta
     5101 ttcgggggcg ctaaagatgc tggaagatct gcgtgccaac agctggagtt tacgcccgtg
     5161 ctgcatggtt cttgcctatc gtgttgctca tttttgttcg gtgtggcgca aaaagaacgt
     5221 cctcaacaat ctgtgggcgg ccccgctgct ggtgctgtat cgcattatca ccgaatgctt
     5281 tttcggttat gaaatccagg ccgccgcgac cattggccgc cgctttacta tccatcacgg
     5341 ttacgccgta gtgatcaata aaaacgtagt agcgggggat gattttacca ttcgccacgg
     5401 cgtcactatc ggcaatcgtg gtgccgaaaa catggcatgt ccacacattg gcaacggcgt
     5461 cgaactcggt gccaacgtta ttattcttgg tgatatcacg attggtaaca acgtcaccgt
     5521 gggcgcgggc agcgtggtgc ttgactctgt cccggacaac gcgctggtgg tgggagaaaa
     5581 agcgcgagtg aaggtaatca aatgaatatt ctgcaattta atgtgcgact ggcggaaggc
     5641 ggggcagcag gtgtggcgtt agatctccac cagcgtgcgc tgcaacaggg gctggcgtca
     5701 cattttgtct acggctacgg caaaggcggc aaagagagcg tcagccatca gaactatccg
     5761 caggtcatca aacatacgcc gcggatgacc gcgatggcga acattgccct gtttcgtctg
     5821 tttaatcgcg atctgtttgg caatttcaat gagttatatc gcaccattac tcgtacaccg
     5881 ggtccggtgg tcctgcattt tcatgtgctg cacagctact ggctaaatct taagagcgtg
     5941 gtgcgctttt gcgaaaaggt gaaaaaccac aaaccggatg tcactctggt ctggacgctg
     6001 cacgaccact ggagcgttac cgggcgctgc gcctttaccg acggttgtga aggctggaaa
     6061 acgggctgcc agaaatgccc gaccttaatt aattatccgc cggtgaagat tgatcgcgca
     6121 caccagctgg tggcgggcaa acgccagtta ttccgtgaga tgctggcgct gggctgtcag
     6181 tttatttccc ccagccagca tgtggctgac gctttcaata gcctgtacgg tccagggcgt
     6241 tgccggatta tcaataatgg cattgatatg gcaaccgaag cgattctggc ggacttgcct
     6301 ccggtgcgcg aaacccaggg taagccgaaa atcgcggtgg tggcgcatga cctgcgttac
     6361 gacggcaaaa ctaaccagca actggtgcgc gagatgatgg cgctgggcga caaaatcgaa
     6421 ctgcatacct ttggtaagtt ctcgccgttc accgctggca acgtggttaa tcacggcttt
     6481 gaaactgaca agcgcaagtt gatgagcgcg ctcaatcaga tggatgcgct ggtgttcagt
     6541 tctcgcgtcg ataactaccc gctgattttg tgtgaggcgc tatcgattgg cgtgccggtg
     6601 attgccaccc atagcgatgc ggcgcgggaa gtgttgcaaa aatccggcgg taaaaccgtc
     6661 agcgaagaag aggtgctgca actggtgcag ttaagcaaac cggaaatcgc gcaggcgata
     6721 tttggtacca cgctggctga gttcagccaa cgcagccgcg ccgcctacag tggacaacag
     6781 atgctggagg agtatgtcaa cttctatcag aatctgtagc tacctgctgc tgccgctgat
     6841 ctacctgctg gttaacgtca aaatcgccca gcttggcgaa agtttcccca tcactatcgt
     6901 cactttttta cctgtcttgt tactgctgtt tttagaacgc atcagcgtta aaaaattgat
     6961 gattgccttg gggattggtg cgggactcac ggcgtttaac tacctgtttg gtcagtcgct
     7021 ggatgccagt aaatacgtca cttcaaccat gctgtttgtc tatattgtga tcattattgg
     7081 catggtgtgg agtattcgtt ttaaaacaat ttcgccacac aaccatcgca agatattacg
     7141 tttcttttat ctggtggtcg ggctggtggt ggcgctggcg gcggtggaga tggcgcaaat
     7201 tatcctcacc ggcggcagca gtattatgga gtcgatttcg aaatatctga tttacagcaa
     7261 cagctatgta ctgaatttca ttaaattcgg cggtaagcgt acgacagcac tttatttcga
     7321 accggcattt ttcgctctgg cattaatctc aatttggctc agcatcaaac agtttggtat
     7381 caaaacgcct aaaaccgatg ctatgattct cgcaggaata atattatccg gatcgttttc
     7441 aggggttatg acctttatcc tgttttattt gctggagtgg gcattccaat atctgaataa
     7501 agaggcgatt aagaaaaagt tgccgttagc attgatttct ctggctgtat tcctggttgg
     7561 tgtggtaatc gcgtttcctt atatttccac ccgtctgggc gatttaggta cggaaggatc
     7621 gtcatcgtat tatcgtattg tcggtccgct ggtaatggtc ggttattctt tgacccatat
     7681 tgacggtgta gtcagatttg gctcacttta tgaatatgtc gcatcattcg gaatatttaa
     7741 cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta tttatttttc
     7801 ctggttcgca gtgtttttat cgctgtggta catggggaaa gtgataaaaa tgatgatcaa
     7861 cgcttttggt gataaccgca attttcgcgt gcaattatat cttttcactc cggtatcgct
     7921 gttttttacc ggttcgatat ttagcccgga atatgcattt ttaatcgtct gtccgtttat
     7981 tttgcgaaaa gcgttaaata ttacgaggta agaataagga acatgttgct tagcataatc
     8041 actgtcgcgt ttcgtaacct cgaagggata gtcaaaacac atgcctcgct ggcgcatctg
     8101 gcgcaggcgg aagatatcag cttcgaatgg attgttgtcg atggcggttc caacgacggc
     8161 acccgtgagt atctggaaaa tctcaatggt atctttaacc tacgctttat cagcgagcca
     8221 gataacggta tctacgacgc catgaacaaa ggcattgcga tggcacaagg caagttcgcg
     8281 ttgtttctca actcgggcga tatttttcat caggatgccg caaattttgt ccgtaagtta
     8341 aaaatgcaaa aagataacgt gatgatcacc ggcgatgcgc tgctggattt tggcgacggg
     8401 cataaaatta agcgtagtgc taaaccgggt tggtatattt atcacagcct gcccgccagc
     8461 catcaggcga tatttttccc ggtatccggc ttgaaaaaat ggcgttatga cctggaatat
     8521 aaagtatctt ccgattacgc gctggcggct aaaatgtata aagccggtta tgcatttaaa
     8581 aaactcaatg gcctggtgtc tgaattttcc atgggtgggg tatcaaccac caataatatg
     8641 gaattgtgtg ctgacgccaa aaaagtccaa cggcaaatat tacatgtgcc tggcttctgg
     8701 gcagaattat cctggcattt acgccaacgt actacctcaa agacgaaagc cttatataac
     8761 aaaagctgaa tataagggaa aacgatgcaa gatttaagcg gtttctcggt gccgaaaggg
     8821 ttccggggcg gcaacgctat taaagtgcaa ttatggtggg cagtacaggc aacaatattt
     8881 gcctggtcgc cacaagtatt gtatcgctgg cgggcttttt tattacgttt attcggcgca
     8941 aaaataggaa aaaacgtagt tattcgtccg tcagtaaaaa ttacctatcc gtggaaatta
     9001 accttaggtg attacgcgtg ggtcggcgat gacgtcaatt tatataccct cggtgaaata
     9061 accattggcg cacattcggt gatatcgcaa aaaagttatt tatgcaccgg tagccacgac
     9121 catgcaagtc aacatttcac cattaatgcc acgcctattg tgattggcga gaaatgctgg
     9181 ctggcaaccg atgtttttgt tgcccctggc gtcacgattg gcgacggcac cgtcgtgggt
     9241 gcacgaagca gtgtttttaa atcgcttccg gcaaatgtgg tttgccgggg gaatcccgca
     9301 gtggtgatac gcgaacgcgt tgaaactaaa taaattcaaa aaatacagag gaataagaca
     9361 tgtcaaaagt cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt
     9421 ttctgctgga aaaaggttac gaggtgcatg gtattaagcg tcgtgcatcg tcattcaaca
     9481 ccgagcgcgt ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc
     9541 attatggcga cctgagtgat acctccaacc tgacgcgcat tttgcgtgaa gtgcagccgg
     9601 atgaagtgta caacctgggc gcaatgagcc acgttgcggt ttcttttgag tcaccggaat
     9661 ataccgctga cgtcgacgcg atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg
     9721 gtctggaaaa gaaaacccgt ttctatcagg cttccacctc tgaactgtac ggtctggtgc
     9781 aggaaattcc gcagaaagaa accacgccgt tctacccgcg atctccgtat gcggtcgcca
     9841 aactgtatgc ctactggatc accgttaact accgcgaatc ctacggcatg tacgcctgta
     9901 acggtattct cttcaaccat gaatccccgc gccgcggcga aaccttcgtt acccgcaaaa
     9961 tcacccgcgc aatcgccaac atcgcccagg ggctggagtc gtgcctgtac ctcggcaata
    10021 tggattccct gcgtgactgg ggccacgcca aagactacgt aaaaatgcag tggatgatgc
    10081 tgcaacagga acagccagaa gatttcgtta tcgcgaccgg cgttcagtac tccgtacgtc
    10141 agttcgtgga aatggcggca gcacagctgg gcatcaaact gcgctttgaa ggcacgggcg
    10201 ttgaagagaa gggcattgtg gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg
    10261 gcgatgtgat tatcgccgtt gacccgcgtt acttccgtcc ggcggaagtt gaaacgctgc
    10321 tcggcgaccc gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag
    10381 agatggtgtc tgaaatggtg gctaatgacc tcgaagcggc gaaaaaacac tctctgctga
    10441 aatctcacgg ctacgacgtg gcgatcgcgc tggagtcata agcatgagta gacaacgcat
    10501 ttttatcgct ggccatcgcg gaatggtcgg ttccgccatc acgcggcagc tcgaacagcg
    10561 cggtgatgtg gaactggtat tacgcacccg cgacgagctg aacctgctgg acagccgcgc
    10621 ggtgcatgat ttctttgcca gcgaacgcat tgaccaggtc tatctggcgg cggcgaaagt
    10681 gggcggcatt gttgccaaca acacctatcc ggcggatttc atctaccaga acatgatgat
    10741 tgagagcaac atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttcttgg
    10801 atcgtcctgt atctacccga aactggcaaa acagccgatg gcagaaagcg agttattgca
    10861 gggcacgctg gagccgacca acgagtctta cgccattgcc aaaatcgccg ggatcaaact
    10921 gtgcgaatca tacaaccgcc agtacggacg cgattaccgc tcagtcatgc cgaccaacct
    10981 gtatggcccg catgacaact tccacccgag taattcgcat gtgatccctg cattgctgcg
    11041 tcgcttccac gaggcgacgg cacagaatgc accggacgtg gtggtatggg gcagcggtac
    11101 accgatgcgt gaattcctgc acgtcgatga tatggcggcg gcgagcattc atgtcatgga
    11161 gctggcgcac gaagtctggc tggagaacac ccagccgatg ctgtcgcaca ttaacgtcgg
    11221 cacgggcgtt gactgcacta tccgcgagct ggcgcaaacc atcgccaaag tggtgggtta
    11281 caaaggtcgg gtggtttttg atgccagcaa accggatggt acgccgcgca aactgcttga
    11341 tgtgacgcgc ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc
    11401 cagcacttac cagtggttcc ttgagaatca agaccgcttt cgggggtaat gatgttttta
    11461 cgtcaggaag actttgccac ggtagtgcgc tccaccccgc ttgtctctct cgactttatt
    11521 gtcgagaaca gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt
    11581 tactggtttg tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag
    11641 cggctgacga tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt
    11701 gtctggcagc acttttatga cgataacttc tccggcacgg atttcaccac tcactatgtg
    11761 gtgctcggtt ttcgcttcag agtagcggaa gaagaactgt tactgccgga tgagcagcat
    11821 gacgattacc gctggctgac gccggacgca ctgctcgcca gtgacaatgt tcacgctaac
    11881 agccgcgcct attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg
    11941 tctacggcat taactactcg ccggagttaa ccggcatcgg caaatacacc ggagaaatgg
    12001 tggaatggct ggcggcgcaa ggtcatgagg tgcgggttat taccgcaccg ccttactacc
    12061 cgcagtggca ggtgggcgag aactattccg cctggcgcta caaacgagaa gagggggccg
    12121 ccacggtgtg gcgctgcccg ctgtacgtgc caaaacagcc gagcaccctg aaacgcctgt
    12181 tgcatctcgg cagttttgcc gtcagcagtt ttttcccgtt gatggcgcaa cgtcgctgga
    12241 agccggatcg cattatcggc gtggtgccaa cgctgttttg cacgccggga atgcgcctgc
    12301 tggcgaaact ctctggtgcg cgtaccgtgc tgcatattca ggattacgaa gtggacgcca
    12361 tgctggggct gggccttgcc ggaaaaggca aaggcggcaa agtggcacag ctggcaacgg
    12421 cgttcgaacg tagcggactg cataacgtcg ataacgtctc cacgatttcg cgttcgatga
    12481 tgaataaagc catcgaaaaa ggcgtggcgg cggataacat cattttcttc cccaactggt
    12541 cggaaatcgc ccgttttcag catgttgcag acgccgatgt tgatgccctt cgtaaccagc
    12601 ttggcctgcc ggataacaaa aaaatcattc tttactccgg caatattggt gaaaagcagg
    12661 gactggaaaa cgttattgaa gctgccgatc gcctgcgcga tgaaccgctg atttttgcca
    12721 ttgtcgggca gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc
    12781 gcaacatgca atttttcccg ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg
    12841 gcgattgcca tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac
    12901 tgaccaatat tctggcagta ggcggtaacg cggtgattac cgcagaggcc cacacagaac
    12961 tgggacagct ttgcgaaacc tttccgggca ttgcggtttg cgtggaaccg gaatcggtcg
    13021 aggcgctggt ggcggggatt cgtcaggcgc tcctgctgcc caagtacaac acggtggcac
    13081 gtgaatatgc cgaacgcacg ctcgataaag agaacgtgtt acgtcaattt ataaatgata
    13141 ttcggggata attatggcgc agtcgaaact ctatccagtt gtgatggcag gtggctccgg
    13201 tagccgctta tggccgcttt cccgcgtact ttaccccaag cagtttttat gcctgaaagg
    13261 cgatctcacc atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc
    13321 ggtggtgatt tgcaatgagc agcaccgctt tattgtcgcg gaacagctgc gtcaactgaa
    13381 caaactcacc gagaacatta ttctcgaacc ggcagggcgt aacactgcac ctgccattgc
    13441 actggcggcg ctggcggcaa aacgtcatag cccggagagc gacccgttaa tgctggtatt
    13501 ggcggcggat catgtgattg ccgatgaaga cgcgttccgt gccgccgtgc gtaatgccat
    13561 gccgtatgcc gaagcgggca agctggtgac cttcggcatt gtgccggatc tacctgaaac
    13621 cggatatggc tatattcgtc gcggtgaagt atctgcgggt gagcaggata cggtggcctt
    13681 tgaagtggcg cagtttgtcg aaaaaccgaa tctggaaacc gcccaggcct atgtggcaag
    13741 cggcgaatat tactggaaca gcggcatgtt cctgttccgc gccggacgct atctcgaaga
    13801 actgaaaaaa tatcgcccgg atattctcga cgcctgtgaa caagcgatga gcgccgtcga
    13861 tccggatctc gattttattc gcgtggatga agaagcgttt ctggcctgcc cggaagagtc
    13921 ggtggattac gcggtcatgg aacgtacggc agatgccgtt gtggtgccga tggatgcggg
    13981 ctggagcgat gtcggttcct ggtcttcatt atgggagatc agcgcccaca ccgccgaggg
    14041 caacgtttgc cacggtgatg tgattaatca caaaactgaa aacagctatg tgtacgccga
    14101 atctggcctg gtcaccaccg tcggggtgaa agatctggta gtggtgcaga ccaaagatgc
    14161 ggtgctgatt gccgaccgta acgcggtgca ggatgtgaaa aaagtggtcg agcagatcaa
    14221 agccgatggt cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata
    14281 tgactctatc gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga
    14341 gggcttgtcg gtacagatgc accatcaccg cgcggaacac tgggtagtgg tcgcgggaac
    14401 ggcaaaagtc accattgatg gtgatatcaa actgcttggt gaaaacgagt ccatttatat
    14461 tccgctgggg gcgacgcact gcctggaaaa cccggggaaa attccgctcg atttaattga
    14521 agtgcgctcc ggctcttatc tcgaagagga tgatgtggtg cgcttcgcgg atcgctacgg
    14581 acgggtgtaa acgtcgcatc aggagctgtt tgtcggatgc ggcgtgaacg ccttatccga
    14641 cctacggttc gggtttgtag tcctgataag acgcggcagc gtcgcatcag gcaatgaatg
    14701 cgaaaccgcg gtgtaaataa cgacaaaaat aaaattggcc gcttcggtca gggccaacta
    14761 ttgcctgaaa aagggtaacg atatgaaaaa attaacctgc tttaaagcct atgatattcg
    14821 cggaaaacta ggcgaagaac tgaatgaaga tatcgcctgg cgcattggtc gcgcctatgg
    14881 cgaatttctc aaaccgaaaa ccattgtgtt aggcggtgat gtccgcctca ccagcgaaac
    14941 tttaaaactg gcgctggcga gaggtttaca ggatgcgggc gtcgatgtgc tggatattgg
    15001 catgtccggc accgaagaga tctatttcgc cacgttccat ctcggcgtgg atggcggcat
    15061 tgaagttacc gccagccata acccgatgga ttataacggc atgaaactgg tgcgcgaagg
    15121 ggctcgcccg atcagcggtg ataccggact gcgcgacgtc cagcgtctgg ctgaagccaa
    15181 cgactttcct cccgtcgatg aaaccaaacg cggccgctat cagcaaatca acctgcgtga
    15241 cgcttacgtt gatcacctgt ttggttatat caatgtcaaa aacctcacgc cgctcaagct
    15301 ggtgattaac tccgggaatg gcgcagcggg tccggtggtg gacgccattg aagcccgctt
    15361 taaagccctc ggcgcgcccg tggaattaat caaagtacac aacacaccgg acggcaattt
    15421 ccccaacggt attcctaacc cgctgctgcc ggaatgtcgc gacgacaccc gcaatgcggt
    15481 catcaaacac ggcgcggata tgggcattgc ctttgatggc gattttgacc gctgttttct
    15541 gtttgacgaa aaagggcagt ttatcgaggg ctactacatt gtcggcctgc tggcagaagc
    15601 gttcctcgaa aaaaatcccg gcgcgaagat catccatgat ccgcgtctct cctggaacac
    15661 tattgatgtg gtgactgccg caggcggcac cccggtaatg tcgaaaaccg gacacgcctt
    15721 tattaaagaa cggatgcgca aggaagacgc catctacggt ggcgaaatga gcgctcacca
    15781 ttacttccgt gatttcgctt actgcgacag cggcatgatc ccgtggctgc tggtagccga
    15841 actggtgtgc ctgaaaggaa aaacgctggg cgaactggtg cgcgaccgga tggcggcgtt
    15901 tccggcaagc ggtgagatca acagcaaact ggcgcaaccc gttgaggcga ttaatcgcgt
    15961 cgaacagcat tttagccgtg aggcgctggc ggtggatcgt accgatggca tcagcatgac
    16021 ctttgccgac tggcgcttta acctgcgcac ctccaacacc gaaccggtgg tgaggttgaa
    16081 tgtggaatcg cgcggtgatg tgccgctgat ggaagcgcga acgcgaactc ttctgacgtt
    16141 gctgaatgag taatgtcgga tcttccctta ccccactgcg ggtaaggggc taataacagg
    16201 aacaacgatg acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc
    16261 tatggtgcaa cgcttttcag atatcaccat catgtttgcc ggactatggc tggtttgcga
    16321 agtgagcgga ctgtcattcc tctacatgca cctgttggtg gcgttgatta cgctggtggt
    16381 gttccagatg ctgggcggca tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc
    16441 gacagaattt gccctgttgc tacaaaactg gaccttaagc gtgattttca gcgccggact
    16501 ggtggcgttc aacaatgatt tcgacacgca actgaaaatc tggctggcgt ggtatgggct
    16561 gaccagtatc ggactggtgg tttgccgttc atgtattcgc attggggcgg gctggctgcg
    16621 taatcatggc tataacaagc gtatggtcgc cgtagcggga gatttagccg ccggacaaat
    16681 gctgatggag agcttccgta atcagccgtg gttagggttt gaagtggtgg gggtttacca
    16741 cgacccgaaa ccgggcggcg tttctaacga ctgggcgggc aatctgcaac agctggtcga
    16801 ggatgcgaaa gcgggcaaaa ttcataacgt ctatatcgcg atgcagatgt gcgacggcgc
    16861 gcgagtgaaa aaactggtcc atcaactggc ggataccacc tgttcggtgc tgctgatccc
    16921 cgatgtcttt accttcaaca ttctccattc acgtctcgaa gagatgaacg gcgttccggt
    16981 ggtgccgctt tacgacacgc cgctttccgg ggttaaccgc ctgctcaaac gtgcggaaga
    17041 cattgtgctg gcgacgctta ttctgttgct gatctccccg gtgctgtgct gtattgcgct
    17101 ggcggtgaaa ctcagttcac cagggccggt tattttccgc cagactcgct atggcatgga
    17161 tggcaagccg atcaaagtgt ggaagtttcg ttccatgaaa gtgatggaga acgacaaagt
    17221 tgtgacccag gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg
    17281 cacctcgctg gatgaattgc cgcagtttat caatgtgctg accgggggga tgtcgattgt
    17341 cggtccacgt ccacacgcgg tggcgcataa cgaacagtat cgccagctca ttgaaggcta
    17401 catgctgcgc cataaggtga aaccgggcat taccggctgg gcgcagatta atggctggcg
    17461 tggcgaaacc gacacgctgg agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat
    17521 ccgcgaatgg agcgtctggt tcgatatcaa aatcgttttc ctgacggtat tcaagggctt
    17581 cgttaacaaa gcggcatatt gatatgagct tacgtgaaaa gaccatcagc ggcgcgaagt
    17641 ggtcggcgat tgccacggtg atcatcatcg gcctcgggct ggtgcagatg accgtgctgg
    17701 cgcggattat cgacaaccac cagttcggtt tgcttaccgt gtcgctggtg atcatcgcgc
    17761 tggcagatac gctttctgac ttcggtatcg ctaactcgat tattcagcga aaagaaatca
    17821 gccaccttga actcaccacg ctgtactggc tgaacgtcgg gctggggctt gtggtgtgcg
    17881 tggcggtgtt tttgttgagt gatgccattg gcgacgtgct gaataacccg gacctggcac
    17941 cgttgattaa aacattgtcg ctggcgtttg tggtgatccc ccacgggcaa cagttccgcg
    18001 cgttgatgca gaaggagctg gagttcaaca aaatcggcat gatcgaaacc agcgcggtac
    18061 tggcgggctt cacttttacg gtggttagcg cccatttctg gccgctggcg atgaccgcga
    18121 tcctcggtta tctggtcaat agtgcggtga gaacgctgct gtttggctac tttggccgca
    18181 aaatttatcg ccccggtctg catttctcgc tggcgtcggt ggcaccgaac ttacgctttg
    18241 gtgcctggct gacggcggac agcatcatca actatctcaa taccaacctt tcaacgctgg
    18301 tgctggcgcg tattctcggc gcgggcgtgg cagggggata caacctggcg tacaacgtgg
    18361 ccgttgtgcc accgatgaag ctgaacccaa tcatcacccg cgtgttgttt ccggcattcg
    18421 ccaaaattca ggacgatacc gaaaagctgc gcgttaactt ctacaagctg ctgtcggtag
    18481 tggggattat caactttccg gcgctgctgg ggctgatggt ggtgtcgaat aattttgtac
    18541 cgctggtctt tggtgagaag tggaacagca ttattccggt gctgcaattg ctgtgtgtgg
    18601 tgggtctgct gcgctccgta ggtaacccga ttggttcgct gctgatggcg aaagcgcggg
    18661 tcgatatcag ctttaaattc aacgtattca aaacatttct gtttattccg gcgattgtta
    18721 ttggtgggca gatggcgggc gcaatcggcg tcacgcttgg cttcctgctg gtgcagatta
    18781 tcaacaccat tctgagttac ttcgtgatga ttaaaccggt tcttggttcc agttatcgcc
    18841 agtacatcct gagtttgtgg ctgccgtttt acctctcgct gccgacgctg gtggtcagtt
    18901 atgcgctggg cattgtgctg aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa
    18961 tagccgcagg cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat ccgctagtgg
    19021 tggaagtgaa gcgtcagttt tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt
    19081 gagttgttgt tttcggttcc gacaacggtt gccgggtgca gcgtaaaacg ccttatccat
    19141 cctacggttc tgtgctcggg acctgtaggc ctgataagac gcgtcagcgt cgcatcaggc
    19201 actgaatgcc ggatgcggcg tgaacgcctt atccggccta cggttctgtg ctcgggtctt
    19261 gtaggcctga taagacgcgc cagcgtcgca tcaggcaaaa agcggcaacg aacaaaacaa
    19321 acgcagcctc acctggcatc aaatttatga ggaaaaaatg aaattactta ttctgggcaa
    19381 ccacacttgc ggcaatcgtg gcgacagcgc catcttgcgc ggcttacttg atgccatcaa
    19441 catcctcaat ccacaagccg aagtggacgt gatgagccgc tatccggtca gttcttcctg
    19501 gctgctcaac cgcccggtaa tgggcgatcc gctgttcctg caaatgaaac aacacaacag
    19561 cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt cgccgctatc aacaccaggt
    19621 attgctctca cgcgtcactg acactggcaa gctgcgtaat atcgccatcg cccagggatt
    19681 caccgacttc gtgcgcctgc tgtcaggtta cgacgccatt attcaggtcg gcggatcgtt
    19741 ttttgtcgat ctctacggcg tgccgcagtt tgaacatgcg ctttgcacat ttatggcgaa
    19801 aaagccgctg tttatgattg gtcacagcgt cggtcccttc caggatgagc aatttaacca
    19861 actggcgaac tacgtttttg gtcactgtaa cgcgctgatc ctgcgcgaat cggtcagtct
    19921 cgaactgatg aaacgcagca atatcaccac tgcaaaagtg gaacatggcg tcgatactgc
    19981 gtggctggtc gatcaccaca ccgaagactt cactgccagc tatgccgttc aacactggct
    20041 ggacgttgcc gcacaacaga aaacggtggc aattaccctg cgcgaactgg caccgttcga
    20101 caaacgtctc ggcaccactc agcaagcgta tgaaaaagcc tttgccgggg tggtcaatcg
    20161 cattctcgac gaaggctatc aggtgattgc gctctccacc tgtacgggca tcgacagcta
    20221 taacaaagac gaccgcatgg tggcgctcaa cttgcgtcag cacatcagcg atcctgcccg
    20281 ttatcacgta gtgatggatg aactcaacga tctggaaatg ggcaaaattc tcggtgcctg
    20341 tgaactcacc gtcggtacgc gtctgcactc cgctattatc tcaatgaact ttgctactcc
    20401 ggcgattgcc atcaactatg aacataaatc cgccgggatc atgcagcagc tgggactacc
    20461 ggagatggca attgatatcc gtcatttact ggacggcagc ctgcaagcga tggttgcgga
    20521 taccttaggc cagcttccgg cgctgaacgc acgacttaac gaagctgtca gtcgtgagcg
    20581 tcagacggga atgcagatgg tgcagtctgt gcttgagcgc atcggggagg tgaaatgaag
    20641 gtcggcttct ttttactgaa attcccgctg tcttcggaaa ccttcgttct taaccaaatc
    20701 actgcgttta ttgatatggg atttgaggtg gagattgtcg cgctgcaaaa aggcgacaca
    20761 caaaacaccc acgcggcatg gacgaaatac aaccttgccg ccagaacccg ctggttacag
    20821 gacgaaccta cgggcaaagt ggcgaagctg cgccaccgcg ccagccagac gttacgcggc
    20881 attcatcgta aaaatacctg gcaggcgctc aacctcaaac gctatggcgc agagtcgcgg
    20941 aacctgattt tgtctgccat ttgtggtcag gtcgcaacac cattttatgc cgatgtcttt
    21001 atcgctcatt ttggccctgc gggagtaacc gcagcaaaac tccgcgaact gggtgtcatt
    21061 cgcggcaaaa ttgccaccat cttccacggt attgatatct ccagtcggga agtgctcaac
    21121 cactacactc ccgaatatca acaactgttt cgccgtggcg acctgatgtt accgataagc
    21181 gatctgtggg ccggaaggtt gcaaaaaatg ggctgtccga gggaaaaaat cgccgtatcg
    21241 cgcatgggcg tagatatgac gcgctttagc ccgcgtcccg tgaaagcgcc cgcaacgccg
    21301 ctggaaatca tctccgtcgc acgcttaacc gaaaaaaaag gcctgcatgt ggcgatcgaa
    21361 gcctgccgtc agttgaaaga gcagggcatg gcatttcgct atcgcatcct cggcattggc
    21421 ccgtgggaac gacgcctgcg taccctcatc gaacaatatc aactggaaga tgtggtagag
    21481 atgccgggct ttaaaccgag ccacgaagtg aaagcgatgc tcgacgacgc ggatgtcttc
    21541 ctgttgccat cggtaacggg cgcggatggc gatatggaag gcattccggt agcgctgatg
    21601 gaagcaatgg cggtcggtat tccagtagtt tccactctgc atagtggaat accggaattg
    21661 gtggaggccg ataaatccgg ctggctggtg cctgagaacg atgctcgcgc actggcacaa
    21721 cgtctggcgg catttagcca actggacact gacgaactgg ctccggttat caaacgtgct
    21781 cgcgaaaaag tcgaacacga ttttaaccag caggtgatca atcgagaact cgccagcttg
    21841 ctgcaggctt tatagaggag aacgcatgcc atttaaaaaa ctctcccgac gcaccttcct
    21901 gacggcaagc tccgcgcttg ccttcctcca tacccctttc gcccgcgcac tacccgcccg
    21961 acaaagcgtt aacattaacg actacaaccc tcacgactgg atcgcctcat ttaaacaagc
    22021 cttcagcgaa gggcaaacag tcgtcgtgcc tggcggattg gtttgtgaca atatcaacac
    22081 cggcatcttt atccctcccg gtaaaacgtt acacatcctt ggaagcctgc gcggcaacgg
    22141 cagagggcga tttgtcttac aggacggcag ccaggtgaca ggggaggagg gcggcagtat
    22201 gcataacatc accctggatg tgcgtggttc tgactgcacc atcaaagggc tggcgatgag
    22261 cggctttggt ccggtaacgc agatttatat cggcggcaaa aacaaacggg tcatgcgcaa
    22321 cctgaccatc gataacctca ctgtcagcca cgctaattac gccatcttac gccagggatt
    22381 tcataaccag attatcggtg ccaacatcac caactgtaaa tttagtgatt tacagggcga
    22441 cgccattgaa tggaacgtgg cgattaacga ccgtgatatt ttgatatctg accatgtcat
    22501 cgagcgcatc aactgtacca acggcaaaat caactggggc atcggcatag gtcttgcggg
    22561 aagcacttat gataataatt acccggaaga ccaggcagtg aaaaactttg tcgtggcgaa
    22621 tatcacggga tcggattgtc ggcagttgat ccatgttgaa aatggtaaac attttgttat
    22681 tcgtaatatc aaagcctgca atatcacgcc ggatttcagt aagaaagcag gcattgataa
    22741 cgccacagtc gctatttacg gttgtgacaa tttcgtgatt gataatattg aaatgattaa
    22801 tagcgccggg atgttaatcg gctatggggt aattaaaggc aaatatctct cgataccgca
    22861 aaatttccga gtgaataata ttcaactgga taacactcac cttgcttata aattgcgcgg
    22921 catccaaatc tccgccggga atgccgtctc ctttgtggcg ctgactaaca ttgagatgaa
    22981 gcgcgcgtcg ctggagttac acaacaaacc gcaacatctt tttatgcgaa atatcaaggt
    23041 gatgcaggaa tcctcagttg gaccagcatt gagcatgaac ttcgacatgc gcaaagacgt
    23101 tcgcggcgtc tttatggcga aaaaagaaac actgctgtct cttgcaaatg ttcatgcggt
    23161 gaatgaaaga gggcaaagct ccgtcgatat cgacaggatt aatcaccatg ttgttaatgt
    23221 ggaaaagatt aactttagat tgccggaacg gagggagtag atttgcgacc attcctggaa
    23281 aaatggagcc atacttagga acaatgctac tgcaatccac aacgaagcgg cgtaacatca
    23341 caagtaattc agtaatcaat tcagggtaat tgatgctggc gaaaaaaatc gaacaagcta
    23401 taattcagca accattttac aggtggatga aataatgacg aatttaaaag cagttattcc
    23461 tgtagcgggt cttgggatgc atatgttgcc tgccactaag gcgataccca aagagatgct
    23521 accgatcgtc gacaagccaa tgattcagta cattgttgac gagattgtgg ctgcagggat
    23581 caaagaaatc ctcctggtaa ctcacgcatc caagaacgcg gtcgaaaacc acttcgacac
    23641 ctcttatgaa ttagaatctc tccttgaaca gcgcgtgaag cgtcagctgc tggcggaagt
    23701 gcagtccatc tgtccgccgg gcgtgaccat tatgaacgtg cgtcagggcg aacctttagg
    23761 tttaggccac tccattttat gtgcacgacc tgccattggt gacaatccat ttgtcgtggt
    23821 gctgccagac gttgtgatcg acgacgccag cgccgacccg ctgcgctaca accttgctgc
    23881 catgattgcg cgcttcaacg aaacgggccg cagccaggtg ctggcaaaac gtatgccggg
    23941 tgacctctct gaatactctg tcatccagac caaagagccg ctggaccgcg aaggtaaagt
    24001 cagccgcatt gttgaattca tcgaaaaacc ggatcagccg cagacgctgg actcagacat
    24061 catggccgtt ggtcgctatg tgctttctgc cgatatttgg ccggaacttg aacacactca
    24121 gcccggtgca tgggggcgta ttcagctgac tgatgccatt gctgaactgg cgaaaaaaca
    24181 gtccgttgat gccatgctga tgacaggtga cagctacgac tgcggtaaaa aaatgggtta
    24241 tatgcaggca tttgtgaagt atggactacg caacctgaaa gaaggggcga agttccgtaa
    24301 agggattgag aagctgttaa gcgaataatg aaaatctgac cggatgtaac ggttgataag
    24361 aaaattataa cggcagtgaa gattcgtggc gaaagtaatt tgttgcgaat tttcctgccg
    24421 ttgttttata taaacaatca gaataacaac gagttagcaa taggatttta gtcaaagttt
    24481 tccaggattt tccttgtttc cagagcggat tggtaagaca attagcgttt gaatttttcg
    24541 agtttggcgc gagtgggtaa cgctcgtcac atcgtggaca tgcatgcagt gctctggtag
    24601 ctgtaaagcc aggggcggta gcgtggaaaa at
//
LOCUS       DAZFAH010000051        24486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFAH010000051 DAZFAH010000000
VERSION     DAZFAH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.051"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..565)
                     /locus_tag="ACHJWB_001955"
     CDS             complement(<1..565)
                     /locus_tag="ACHJWB_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFO4313600.1"
                     /translation="MYKIRLQKNITILFIFIVMLSFSYWLANSFGMKWGVLAIIGFFL
                     GFTLSFSRFGIVAGWRDMLTMSNSYYVRIHLITIALEVVLFTLCLSFSHAFFGGKMVG
                     NITAISIPFLVGAFVFGIGMQIAGACASGTLYCCGEGRPAFWFVLCCYGIGTLIGNHY
                     KPVLKPIFPGEIITTKDITGGLWSGMLL"
     gene            complement(1301..1468)
                     /locus_tag="ACHJWB_001956"
     CDS             complement(1301..1468)
                     /locus_tag="ACHJWB_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4313601.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            complement(1470..1772)
                     /locus_tag="ACHJWB_001957"
     CDS             complement(1470..1772)
                     /locus_tag="ACHJWB_001957"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4313602.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            1857..2243
                     /locus_tag="ACHJWB_001958"
     CDS             1857..2243
                     /locus_tag="ACHJWB_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313603.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLHGMD
                     SFNSRYQIESTRRTLEAMVRDGLLERINVYERREGAGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVEGQ"
     gene            complement(2690..2983)
                     /locus_tag="ACHJWB_001959"
     CDS             complement(2690..2983)
                     /locus_tag="ACHJWB_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4313604.1"
                     /translation="MMTDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALP"
     gene            complement(3003..3419)
                     /locus_tag="ACHJWB_001960"
     CDS             complement(3003..3419)
                     /locus_tag="ACHJWB_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO4313605.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKRQRKNRRRINRLAEYWPE
                     LFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHTTRYLKAIVAGGARY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(3436..3885)
                     /locus_tag="ACHJWB_001961"
     CDS             complement(3436..3885)
                     /locus_tag="ACHJWB_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4313606.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(3882..4331)
                     /locus_tag="ACHJWB_001962"
     CDS             complement(3882..4331)
                     /locus_tag="ACHJWB_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313607.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(4619..6445)
                     /locus_tag="ACHJWB_001963"
     CDS             complement(4619..6445)
                     /locus_tag="ACHJWB_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO4313608.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGRLA
                     GDNVPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDA
                     DYYPEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGA
                     SQRGEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQN
                     AIKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPP
                     AEGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSG
                     KSVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAI
                     TGGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENE
                     RDPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCG
                     YLMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMA
                     EYGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(6622..6813)
                     /locus_tag="ACHJWB_001964"
     CDS             complement(6622..6813)
                     /locus_tag="ACHJWB_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313609.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            complement(6858..7067)
                     /locus_tag="ACHJWB_001965"
     CDS             complement(6858..7067)
                     /locus_tag="ACHJWB_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313610.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            complement(7071..7256)
                     /locus_tag="ACHJWB_001966"
     CDS             complement(7071..7256)
                     /locus_tag="ACHJWB_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313611.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(7249..7419)
                     /locus_tag="ACHJWB_001967"
     CDS             complement(7249..7419)
                     /locus_tag="ACHJWB_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4313612.1"
                     /translation="MAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARARLPHAHLI
                     FVARIRQEVTHA"
     gene            complement(7474..7557)
                     /locus_tag="ACHJWB_001968"
                     /pseudo
     CDS             complement(7474..7557)
                     /locus_tag="ACHJWB_001968"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7891..8079
                     /locus_tag="ACHJWB_001969"
     CDS             7891..8079
                     /locus_tag="ACHJWB_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313613.1"
                     /translation="MTNEELQEIALSILERQREKAQRQRRLLLNPLPLTRPTRFALED
                     FVEKYPRRLKATKNRPPG"
     gene            complement(8386..9234)
                     /locus_tag="ACHJWB_001970"
     CDS             complement(8386..9234)
                     /locus_tag="ACHJWB_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO4313614.1"
                     /translation="MKRPNMPVQGQGFVQSEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTTWIKGRIEEYGFKKGVDFEVVENLYFDSPKRGSQSVNIEQFDS
                     GWTTKRGGDRRSEDYLISTNMAKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAA
                     LRRELKARVTVASFFKPMCAALELYRAELGKETHQHHYTTESNMLARIVLGGMTAKQW
                     AQANGIKGKPRDSMSVMQLEHLSYLDQSNITLIELGMTYQQRKAELIRLSQRWLARRM
                     GEHEHA"
     gene            complement(9302..9517)
                     /locus_tag="ACHJWB_001971"
     CDS             complement(9302..9517)
                     /locus_tag="ACHJWB_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4313615.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(9904..10554)
                     /locus_tag="ACHJWB_001972"
     CDS             complement(9904..10554)
                     /locus_tag="ACHJWB_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313616.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDMEALMKEKGISPKGLAKSTFMQKISAALLAVSQAD"
     gene            complement(10551..11813)
                     /locus_tag="ACHJWB_001973"
     CDS             complement(10551..11813)
                     /locus_tag="ACHJWB_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4313617.1"
                     /translation="MPKLTDIQIRAWIKSGERFEGRSDGGGLYLRYRKEDQIPTWRFR
                     YKFAGKARAMVIGSYADYSLSKARETAKELSARVALGYDVAAEKQERKAEALAKIEAE
                     KKAVRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTTLLVSLERGEHYNVTPIRRAKS"
     gene            complement(12003..12866)
                     /gene="yicC"
                     /locus_tag="ACHJWB_001974"
     CDS             complement(12003..12866)
                     /gene="yicC"
                     /locus_tag="ACHJWB_001974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4313618.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            12993..13709
                     /gene="rph"
                     /locus_tag="ACHJWB_001975"
     CDS             12993..13709
                     /gene="rph"
                     /locus_tag="ACHJWB_001975"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4313619.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13774..14415
                     /gene="pyrE"
                     /locus_tag="ACHJWB_001976"
     CDS             13774..14415
                     /gene="pyrE"
                     /locus_tag="ACHJWB_001976"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4313620.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14452..15048)
                     /gene="slmA"
                     /locus_tag="ACHJWB_001977"
     CDS             complement(14452..15048)
                     /gene="slmA"
                     /locus_tag="ACHJWB_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4313621.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15155..15613)
                     /gene="dut"
                     /locus_tag="ACHJWB_001978"
     CDS             complement(15155..15613)
                     /gene="dut"
                     /locus_tag="ACHJWB_001978"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4313622.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15591..16811)
                     /gene="coaBC"
                     /locus_tag="ACHJWB_001979"
     CDS             complement(15591..16811)
                     /gene="coaBC"
                     /locus_tag="ACHJWB_001979"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4313623.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16983..17651
                     /gene="radC"
                     /locus_tag="ACHJWB_001980"
     CDS             16983..17651
                     /gene="radC"
                     /locus_tag="ACHJWB_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4313624.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIDELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17868..18104
                     /gene="rpmB"
                     /locus_tag="ACHJWB_001981"
     CDS             17868..18104
                     /gene="rpmB"
                     /locus_tag="ACHJWB_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4313625.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELCARGEKY"
     gene            18125..18292
                     /gene="rpmG"
                     /locus_tag="ACHJWB_001982"
     CDS             18125..18292
                     /gene="rpmG"
                     /locus_tag="ACHJWB_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4313626.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18390..19199
                     /gene="mutM"
                     /locus_tag="ACHJWB_001983"
     CDS             18390..19199
                     /gene="mutM"
                     /locus_tag="ACHJWB_001983"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4313627.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19238..19717)
                     /gene="coaD"
                     /locus_tag="ACHJWB_001984"
     CDS             complement(19238..19717)
                     /gene="coaD"
                     /locus_tag="ACHJWB_001984"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4313628.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19725..21002)
                     /gene="waaA"
                     /locus_tag="ACHJWB_001985"
     CDS             complement(19725..21002)
                     /gene="waaA"
                     /locus_tag="ACHJWB_001985"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4313629.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21415..22473
                     /gene="rfaQ"
                     /locus_tag="ACHJWB_001986"
     CDS             21415..22473
                     /gene="rfaQ"
                     /locus_tag="ACHJWB_001986"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4313630.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22470..23594
                     /gene="waaG"
                     /locus_tag="ACHJWB_001987"
     CDS             22470..23594
                     /gene="waaG"
                     /locus_tag="ACHJWB_001987"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4313631.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23587..24384
                     /gene="rfaP"
                     /locus_tag="ACHJWB_001988"
     CDS             23587..24384
                     /gene="rfaP"
                     /locus_tag="ACHJWB_001988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4313632.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5760 a         6179 c         6042 g         6505 t
ORIGIN      
        1 ttaataacat gcctgaccac aagccgccag taatatcttt ggtggtaata atttctccgg
       61 gaaaaattgg ttttagcact ggcttatagt gattgccaat taatgtccct ataccatagc
      121 agcacaaaac aaaccaaaat gccggacggc cctcaccaca acaatagagt gttcctgacg
      181 cacatgctcc ggcaatttgc attccaatac caaaaacaaa cgcacccacc agaaaaggga
      241 tacttattgc tgtaatattc ccaaccatct tcccgccaaa aaaagcatgg ctgaatgaca
      301 ggcagagtgt aaacaaaacc acctcaagag cgatggtaat taagtgaatc cttacatagt
      361 atgagttgct catggtcagc atgtctctcc aacctgcaac tatcccaaat cgactaaaag
      421 acaatgtaaa acccaagaaa aaaccaataa ttgcaagtac gccccacttc ataccaaaac
      481 tattagcaag ccagtaacta aatgacagca tgacaataaa aataaataat atagttatgt
      541 ttttctgaag ccttatttta tacattttta atatacctat atgtttccac gtgatgcgct
      601 gatgtacttg ctgaaaagga atcgttccag aaatgtatct acgcaatatc ctgaacacgt
      661 tacccaaaat gcctgttcca gaatgatact cacctataaa taaccgtcaa tatgacgctc
      721 aagtactttc tgtgagataa tgcagcgaat tgatactgac aattcaacgt ccgtcatcgc
      781 tcccccaatg tgttgaggtt aggtagaaat gataacaaat acaccactcc tgcccctgta
      841 acctcccctt ttttatacgc tcatttttgc gcactttttc gatcatcacg tatcggtttc
      901 gttttgcgct tcctgttgcg tttcgttttt gagaaaaatg cgcagttttt tgcgcacttt
      961 ttgaggtgtc tatccccctg caagccgcgt catatctggc tttgctctgt ttgtctgtgt
     1021 gatcgatttt gtgatcagtt ttatgatcga tttgcgcact tttttgcgca ttttttcagg
     1081 gatttttcac cccatcgaag aggcttctaa ggggctgtga agtgggtagc attttgctgt
     1141 gatggaagga ctaccgctac tgtctgataa tggtgacgga ggccgctatg ttcggctctg
     1201 attgcagcct gccgtatatt caccaattta cctggctgtt atttccataa cccgtaatgt
     1261 cgccgatccc gtctgattcc catatcgtca acagtcttat ttacagcatc ctgaagttcg
     1321 caaggcatat ttcccgccgt ctggctatat gttctcgctg cggatcggtg tgggcctctt
     1381 tcattacgtc aagccaacga gcggcggctc ttcgccatag tccctgagat tccagttgtt
     1441 cggctttttt atcagttatt gtgtccatat taacttccgt ctcttcgccc tgtttcaccc
     1501 ctgtacttcg tgccccgtaa aggatcttct tttttctggt caatccccat atctttaagc
     1561 gttctggtcg ctgctgccct gacggcgcat acatgccccc actctgttgt ttttggtctg
     1621 gtggcttttg ccgtgcattg cgcccttcgt tgggtaatat agtgcctctc ttcatcagtt
     1681 tttgcctgtc tgaacaattc tccccagcgt tcagcagcac gccgccagag gccgctattc
     1741 tccagcgctt cagccttgtc atcacgtatc atctgtaccc cgccgattct gtcctttcaa
     1801 aatttgtctt atggcttatt gtatgaggga ttcatcctac aacaagaaag gtcactatga
     1861 gactgacgca atacaaaaaa gagatcctca gttacttcac tcccgacaat ctggcgtggg
     1921 ttactgccga aattggcagt ccgccgtttg atgtctccgg tgtggcttac ctgctgcatg
     1981 gtatggattc attcaacagc cggtatcaga tcgaatcaac aaggcgcacg ctggaagcta
     2041 tggtgcgtga tggtcttctt gagcggatta atgtgtatga gcgtcgtgag ggcgctggtg
     2101 atggtgttcg tcgaaaggtg gcgcgttatg gcctgcctgg tcattctgtt gtggtgcgtg
     2161 acggtgaggg ccggaacggt gcaattgacg gcgaatgtat acgcatcaat gctacagatg
     2221 tggtgctggt ggaggggcaa taaaaaaccc tgaaagattt ctccgggtgc tccagttgca
     2281 cgtggcagaa atccttcagg gtcagccgta acgggcctct gtacagaggg ctccagttgc
     2341 ccagagatga gcacccgcta cagcctgttg tgataagccg ctttacagtg agctccagtt
     2401 gctcaaagag gcgcgttttt atcacaacct gaccgcgata acccgctgta cagagggctt
     2461 caattgccca aagaggcgca ttcttatcgc ggcatgacga aatcaccgtg atgttttcgt
     2521 cgttcactca tcccgatgtg tgggagatgc accggaatga gtgccgaaaa gccgctggct
     2581 ggcttcatgc agtcggactc agttgcccgg ggcgaacacc atcaacggcc ttttggtggt
     2641 gattatagcg aatcgcgata attgagcaaa tacggtgatg agatgcgtat cacggtagcg
     2701 ctaaatcgtc gcctgcatca ctgaagctgt aatttctcca ttcatcctgc ggattaatcc
     2761 ccatctcttt cagcgttctg tctgcggcct ttttcacatc gccaaaggtg acgaactgct
     2821 caggcatacg acgggtttta ctgatgcata tcgcccgacg tctggcagct tcctcgcgtt
     2881 cactgtccgt ttctgcgtga agaagaacct cagcccatcg ggtcgccgca cgtcgccata
     2941 aaccgcgctc ctcaagtttc tgtgccacgc tgtccgtcat cactggcttt acctccggct
     3001 gattactgct gactgttttt ctgcttcatc tgctccagtc tccgcttcag tagttcgctg
     3061 gctactgatt tatcttcttc cgtgacttca ccgcaaggtt gcccgttcag tccataccgt
     3121 gcgccgccag cgacaatggc tttcaggtat cgcgtcgtgt ggatgtatga ggttaatgcg
     3181 ccacgtaata cgccatgacc aatcgccagt tcccgcgcct tcgcgtcctg caacatatcg
     3241 tgtatcacgc cagccttaag gggttttata ttctctgccc caaacagttc aggccagtac
     3301 tcagccagtc ggtttatcct cctgcggttt tttcgctgcc gctttgtcat gtgctgccac
     3361 ggtatgcgcc gtgatggttt acgtgtggtc tggtcctgtt gtgtggtctg ctggttcatg
     3421 ttgtgctcct gtcattcaga acggaatatc gtcagagaac gggtcatcac ctcccgctgg
     3481 tggctgattc ccccgtgttc cggtctgtcg ttttgctctt tccagtgctt cggtcgcctt
     3541 cccctgttgc ccccgcttcc cgccaggacg cgccgaacgg gcactgatta cgctgtcagc
     3601 gataacctgc cagccctgcc gtatttctcc gttctgtccg gtccactgac tgatctgcat
     3661 gttacccgcc acgctgataa ggtcgccttt ttcatgtctc gccagcgcgt cggcctgctt
     3721 accaaatgcc aggactgata gccataacgt cgcctgtccg tcctgtgcct gactgcatgg
     3781 caaagatacc gccatacgcg ccagcgtcat cggtgtgccc ttgctggtct gttttacctg
     3841 cgggtcgtcc accagccgtc cgtaagctgc aatctgtgct gtcatggtct gcgctccttg
     3901 ttccggtact cttggataat acccattgtc atatcgaata aatcacatgc cgctttttct
     3961 gcatcttccg ctttcaggtc atacagcgtc atgaaatggt aagtgatcgc ccgtgctgct
     4021 cttttgctgt ttgctgcata tttctcatag atttttcctg cccgctcttc aatatcccgt
     4081 cggaactctt cacgttcaaa caggtcacgg tgtatcaaca tcagcgaatc attcagccat
     4141 gtcacatcgg cgtataccgt tcgcttccag acaatcagga tcggaagttt atgtttgtcg
     4201 cagtgatgca gatgttcgtc aggtatataa agcgtggctc tgcctcgtac cgatacgaac
     4261 ctcacaacag attccggttc ggttaaatcg atagcgtgtg aatgtgattt tttggcgtgt
     4321 ttctctgtca taaaattttt ctcaaaggaa taaataaaaa tttatgccca aaaggtgttc
     4381 aactgttcac cttttgctat ttcgccttta gattcatgaa gttaaggggt gaacgctttt
     4441 tgtaaaggtg ttcaccaact gttcaccctg ctcacccgtg actgttcaaa aatcatacaa
     4501 aaaggtgaac aggtgaatac tttggtgaat agttttacaa aaagtgttca cccattaaca
     4561 tattgatact aaatacattt attgcagggt gaacagtggt gaacagtttt tctataactt
     4621 aatttttatc accctcattt ttcactggtg cacatgacgg cagccagtcc tcagactcat
     4681 ccgtcagtat cacgtttgat cgcacacctt ctttagttct ctttttcata tactccctgc
     4741 catattccgc cattgcgcct ggcatatctg caccgaagcg cgtcagtgtt acgggcttac
     4801 tgaaaccgtg cgccctcata tacgagagat aggcgtgata aagataacgg cgtggactga
     4861 aaggaataat ctcagcatta ccgactaaca ggccgtcaca cattaccgac gacatgagat
     4921 agccgcagaa gtccaccagc gaatccccct cacgctttat cgccagtgct tcttctgatt
     4981 tctgctgctc atataaaagg cgtctggctt cgtcctgatc agcaaaccgt gtaagcagat
     5041 gacgaatcac tacagcaagc tcaccttcta ttttttccgg cagcatcgga tcgcgttcgt
     5101 tttccggtac aacctccgaa aaattgaata tcacacgacg acgtgagatc cccccgctgc
     5161 ggtcactgaa tgacatggcg ctgttgttaa ccgccagcac aaccgccgga atacgcgttg
     5221 agtatggcgc tttgtgtttc gggtcaattg ccaccttgtc accgcccgta atggccttta
     5281 tccctgcacc atcacccgcg tagcgggtca tatccggcag aataatcagt gaaaagccaa
     5341 ccactaatgc gcgttccctt gcatcctcca gtgccttcat gcttgccgat accgtgttgg
     5401 ccttacctgc cagcatggta caaatctccg ccataacact tttaccgctt ccaccaggac
     5461 ctgttacctc aatgaataac tgccagtcgt accggttcgc cagcaccata aataacgctg
     5521 ccagaacgcg atctgccttg cggtcattct cagccaccga acggcgcaac catttccaga
     5581 aattcggcgc atgtgttgcc agcgtttccc cctctgctgg tgggctgaaa ggtaattcac
     5641 tggcaattaa caaccagtcg tttttgttat gctcccgaaa attacctgtt ctggtatcaa
     5701 ataccccgtt actgaatcca atcaggttac gggctgtatt ccccattaca ggcaaactta
     5761 acttcatggt atcgaccgcc gatttaatgg cgttctgcga atagctgatc tccgcatcaa
     5821 tgaaaatctg tgccatagct cgctgtaatt ctttatcctg tactggctcc catacaacgc
     5881 cgttgtaatg gtgaacagtg tcagagtcag catgaatcgc cagttcaccg ccataatgtg
     5941 ccaggagaac ttcgccgcgt tgacttgctc ccatctggtt aagcgctagt gatgaagcgt
     6001 tatcgtcttt tacccgctct tttttcttta caggcagttc aactaccttt ttcttttccg
     6061 cctgctctgc ccgttcacgt tccagatatt cgcgccagtt ctcccgtttc tggctgtgca
     6121 ttccttcagg gtaataatca gcatccctga cacctgccac tgccagtttc tgcccgatgg
     6181 tattaacaag ccccggacgc aataaccccg cctgatagag acgcacgcga tagcgtccgt
     6241 ccggtacgat ttgcaggttg tccagttcgg caagttgttg ctctccaagc cagacaggtg
     6301 gcacgttatc gccagccagt cgcccgtcct gttcctgcca ctgcttcgca tgtgcccacg
     6361 catcactgcc agcgaaaata atcacttccg tatcggcgtg ttttttccct cgaggctgtt
     6421 tttttaagtt cggtgcgctt ttcatttctt acctctgctg atcatcgtca tgtggctacc
     6481 gtattcattg cggtaaatat ccccgcacat aaattcgggg tgagttttgc tactggcagt
     6541 taagccagta ttttttgatg ccattgaatt aatctcctga aggagtcgtc ttaatcctta
     6601 tttcccgttt atttttagcc gttattgttc gccgcgtttt tcttcctccg tcattaacca
     6661 caccgccaca ttgctggaaa gttgtttgat aagtcctgca ataacagaaa ggtcatggtc
     6721 gtctatttct tcgtggttgc gttcaatcat gcggctgatt acttcgacct gttgcgcttg
     6781 cagtgaggct tctgccagtg ttatttgctg cattactgca cccccttaat ttcatgatat
     6841 tcagaacaac aaaggattca gtcattgacg catattgcat catcacccga tgcagcaatc
     6901 agccagctac ctgccttacc tgtaagctgc ttaatcaggg cgagcaatga ctcaatctca
     6961 ccctcagtca ttccacctgt gttacccatc atcagagaac aaataagttc tgcctgcatt
     7021 gcgctttttg ctgcttcatc aacagtcata tcggaaaaat cacacatgaa ttactcctca
     7081 tcctgaatac atgtaactgc gtggataatt acgccggata aaccggcttt agctgatttg
     7141 ttaattactg tgctgattgc agcctggacg ttttcggcct tcacgttctg tgtgatgccg
     7201 atggtatggc cgcgcttgtt aacagcacgg gaaaagacgc ggaaggtttt aagcatgagt
     7261 cacctcctga cggatacggg cgacaaaaat cagatgtgcg tgcggcagcc gggcgcgtgc
     7321 ttcgcgctca gtggcagcgg taacggtgaa tattgcaatg ctgtctgtca ggcactgcat
     7381 aaagcgccag acaaagtaag ggcgtgcgga tacagccatg tgaaaggctc ctctgacttt
     7441 gggattatac ccaccgccca agaggccaat cttgctggcg gtggactgta cggaattggc
     7501 cttactggtg ttagaggaaa ccagccttcc cgaaggaagt tccatacagc ccaccattgt
     7561 tgaggtgtgc gtgtgcgccg atacaaaaaa aaagacgctg gcgcgtcgtg tatcgcctct
     7621 aactccagcg ggaggccaat cccgatcccc gttttataag ggatagcctc agtatatgcc
     7681 gcttttttgc ttaagtggtc ggatgtgttg cgagagaagt ttgtgcgaaa aatacctgcg
     7741 ttttctttga aagcacataa taacatcacg ttatttatgt ttatgcagtg tgtcgcaaag
     7801 cgacctgaat catcacgcaa attacaacca taaaaaatac ctgcgctgtg atatgaggta
     7861 ctacagcgca ccgctttacg ggtacagaca atgacaaatg aagaattaca ggaaatcgca
     7921 ttatccatac ttgagcgaca gcgcgaaaaa gcacaacggc aaaggaggtt gttacttaat
     7981 ccactgccac tcaccaggcc aacacgattt gcccttgaag attttgtcga gaaataccca
     8041 cggcggttaa aagccactaa aaaccgaccg cctggctgat agccttgtcc accagctaca
     8101 ctcatggcat agttggtgat gcgcttgatt tcctttatct ccattggcga cccttccgtg
     8161 gtcgcctttg ttttgcctgc cattcctgca tcatgcggat attcgcgcca tacagggcca
     8221 ccagcgcgat aatttaataa aacctccttc tgataatgct taacgtgttt catgcgctca
     8281 gcggcgaaat ggctcacgat atggtgcatg taatcagaat ggttgaggct cagttgtgcg
     8341 aacgtatccg ccgtgtcgat gtacagggcg gcagcgttgc ggatgtcagg catgttcatg
     8401 ctctcccatg cgacgggcca gccagcgctg tgaaaggcgg attaattcgg cttttcgctg
     8461 ctggtaagtc atacccaact caatcagcgt gatattgctc tggtcaaggt aggaaaggtg
     8521 ttcaagctgc ataacgctca tgctgtcacg cggttttcct ttgatgccgt tcgcctgcgc
     8581 ccattgtttc gccgtcatgc cgcccagcac gatacgcgcc agcatatttg attctgtggt
     8641 gtagtggtgt tgatgagtct cttttcccaa ctctgcgcgg tacaactcca gcgcagcgca
     8701 cataggctta aaaaaactgg caacggtgac acgcgctttc agttcacggc gcaaagctgc
     8761 ggcacgttcc ggcgctgcct tgtgtagctc ttcttcacat ttgatgaagt actggcgaat
     8821 tgcgcggccc tgttcagtgc gttcgaccat cgccagttct ttagccatgt tcgtggagat
     8881 caaatagtct tcactgcggc gatcaccgcc gcgctttgtt gtccagccag aatcaaactg
     8941 ttcaatattt acactttggc tcccccgttt cggggaatca aagtacaaat tttctacaac
     9001 ctcaaaatcc acaccttttt taaatccgta ctcctcaata cgccctttga tccaggtagt
     9061 gaaatcacgg cctacaccga gcgctttatg cagtgcgcgt gcgctgacaa tgcccgtttc
     9121 atatccgcca ataatgccag gtgataccgg aataattgcg gcgaaatact gttggctcag
     9181 ttcttcggac tgaacgaagc cctgcccctg tacgggcata ttcggacgtt tcattgaaaa
     9241 ctcctgctgt cgaatcaggt ttggtgctga cgggcgcgga actgtacgcc cgtcatgctg
     9301 atcactgctt ggtgttgttg cggcgctcgt tcagccagtg gttgatctcc accgcattaa
     9361 atacggtgtt tttctccccc agcttcgtgg gcttcggtaa tttgccgata cgcacccagc
     9421 ggtcgatagt tgacacgctg gtatcaagca acttagccag gttgcagcgg cggatgtagc
     9481 ccgtggtagg gatagcttct tgatggttag ctgtcatgat tccctcttgg gttaaaagtt
     9541 gatgttttct ctattctatt ggttgtttgt acagcattcc atcaaatctg gaatctatca
     9601 aaatattcac agattctatt gaaataatat atttccagta aaagattgag tgagcgggaa
     9661 gaggtgctaa gactttgaaa gtttttgctt tattggcgat aggtcactga atccgtatac
     9721 atcctgatac cgtgatatac ccaatgcgtc gttatgcgct gttatggttt gccatctttt
     9781 gccagttttt aacgtgttaa tcctgtttat accgatttat aacgattaat aacggtgaat
     9841 aaattttgct ggtgggggat gtatggggat tacagccccg tgaatacagg gcgtgttggc
     9901 tggttaatct gcctggctta ctgccagtaa tgcggctgat attttttgca taaacgtgga
     9961 tttggctagc ccttttggcg atatcccttt ctccttcatc agcgcttcca tgtcgcggta
    10021 aatggctgaa cggtttggtt ctccgtcctg aatgtatgcg gttgtgcctg attttttcac
    10081 gaggagtaaa accagcaacc ccatcatctt catgtttccc tgttcttcgt cgagtttcct
    10141 gtgctgcccc cttccgctgc ggcttgtttc tttgatgtaa cggaccagat cccgcccgcc
    10201 aagattaagg gctttttcct cccagttttt tgtcccacgc aaatcatcga tagtatctgt
    10261 taccgccttg attattgcct ctggcgtgcc tgcctccatg aacgggaagg aagcaaggaa
    10321 tatatccccc gctctgtatg gctcatcctt gcttgttttt actccactat gcagcctgat
    10381 agcacgtgcg atatgagttc tggctgattc gacctgttcg aacttgtcct gcggtacgtc
    10441 gcctatcctc attgacggat taagatcagc taacgcaagg gccatttcgt ggggagtgat
    10501 tgaaacagaa gcgcaaaggc gctctattgg cgatttcttc aggatgttaa tcatgattta
    10561 gcccttctga ttggtgtcac gttgtagtgt tctccgcgtt ccagcgacac taacagagtg
    10621 gtccacttcg ccagtgcgtc ccgtcgttca ttgaaatact gatagcggtt gtatatgcct
    10681 tcgactccct tgatgcggtg attgaggcat ctttcagcga ctaccggatc aacaccaagc
    10741 gcagctagat gtgtgcgtgc tgtacggcgg aagtcgtgaa tagtgaaatt tggaacttcc
    10801 ggcatctctg cgcggacttt tgccagcgca acaggtaggg tgctctcctg aatgtggggg
    10861 atcatcctgt gctgcatttt ccgcgccgga agcacccagg cactattgca tgacaacaca
    10921 cgcaactctc gcagccattc aataacggga tggacgagtg gaatatcgat ggggtcgccg
    10981 tttttgctgc gttcctccgg cagatgccat acagcgttat caaggtcgaa ttcttcccat
    11041 ctggcggcgc acaactccat cttcctgaca caaagcgcca gcaatagctt aaatgtgagt
    11101 tcattctcgc ggctgaaacc ctttgcggtc cgcatagcct gaaagaatcg gattagctct
    11161 tcacgagtca gccaacggtc gcgggaggac tccttgccac cagcatcaga gacttcaaac
    11221 gcagagcatg ggttaatctc cagcacatga cgttttaccc cgtagtcgaa tatgcggcgc
    11281 gtccagcgta gaacgtccgt tgcaatggtt ggcgctccac ggtcaacaat tgcctttagc
    11341 atctcgtcaa tgtgtcgtgg ttttacgtcc tcagccttca tatgaccaat acgcgggttt
    11401 atgtctttgt cgatgcgacg gcgcaggatg tcgggatgtt tccatcgtgg cagtatctga
    11461 cgttcgaaat attcggcagc aagctctgat acgcgcacgg cctttttttc tgcctcaatt
    11521 tttgccaggg cttcggcctt acgctcctgt ttctctgctg ctacgtcgta cccaagtgcg
    11581 acgcgagcgg atagttcctt tgctgtttct ctggcttttg atagcgagta atcagcatag
    11641 gaaccaatga ccattgcccg tgcttttcct gcgaatttgt agcggaatcg ccatgttggt
    11701 atctggtctt ctttccggta acgaaggtat aagccaccgc catctgaccg cccttcgaat
    11761 cgttcacctg attttatcca tgccctgatc tgaatatctg tgagctttgg cattaagaac
    11821 tcctgaacat tgaatagaga ggtgtaccca aacagatgtt ctaaaatgca attgggtaca
    11881 cctttgggta cacgctaatc atgcgctttc ttgcgtcgtt atgatagcca ttaattacaa
    11941 aaaaacccgc attatcgcgg gtttgattga attattgcgc tttattgatt ccctctgata
    12001 gcttactcca cattttgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc
    12061 gcggagtttg tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg
    12121 aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg
    12181 taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt
    12241 gccagcagaa ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc
    12301 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
    12361 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga
    12421 ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt
    12481 tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
    12541 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
    12601 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aacatctggc
    12661 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga
    12721 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
    12781 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
    12841 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
    12901 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
    12961 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
    13021 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
    13081 tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct
    13141 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
    13201 cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa
    13261 tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt
    13321 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga
    13381 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc
    13441 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggtatt gtaaacggtg
    13501 aagcaatttg cgatctggaa tacgttgaag actcagccgc agagaccgac atgaacgtag
    13561 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca
    13621 cccatgaaga gctactcacc ttgttggcgc tggcccgagg gggaatcgaa tccattgtag
    13681 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
    13741 tgtctgtaga aaagtaagat gaggagcgaa ggcatgaaac catatcagcg ccagtttatt
    13801 gaatttgcgc ttagcaagca ggtgttaaag tttggcgagt ttacgctgaa atccgggcgc
    13861 aaaagcccct atttcttcaa cgccgggctg tttaataccg ggcgcgacct ggcactgtta
    13921 ggccgttttt acgctgaagc attggtggat tctggcattg agttcgatct gctgtttggc
    13981 cctgcttaca aagggatccc gattgcgacc actaccgccg tggcgctggc ggagcatcat
    14041 gaccttgacc tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg tgaaggcggc
    14101 aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt gatcaccgcc
    14161 ggaacggcga ttcgcgaatc gatggagatt attcaggcta atggcgcgac gcttgctggc
    14221 gtattgattt cgcttgatcg tcaggaacgg gggcgcggcg agatttcggc gattcaggaa
    14281 gttgagcgtg attacaactg caaagtgatc tctatcatca ccctgaaaga tctgattgcc
    14341 tatctggaag agaagccgga aatggcggaa catctggcgg cggttaaggc ctatcgcgaa
    14401 gagtttggcg tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat attactgcaa
    14461 ctgcgcggca attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt atttaaattc
    14521 gctgcggaca aaacgtgaca gcataccttc acagaaggcc aggatctggc ttgccagcag
    14581 ggtttcatcg gtggtgtaac cttcaccctc acgcattctc ttttcacgca atacctgacg
    14641 cagctgcgct tcaatacgct cgaacagctg gttgatacgc ccttgcaggc gatcctgttc
    14701 aaacatcagc gcatgaccag tgaggatgcg ggtcaggcca ggattacgct caccaaaacc
    14761 gagaagcagc aacacaatca gacgcaggcg cgctgtggtg tctttctcat ctttcagaat
    14821 caggttgatg cgagtaatca ggctatcttc gataaactca atcaggctat cgaacatgcg
    14881 ggtcttactg gggaagtggc gatacagtgc cgcttcggaa acgccgacag aggcggccag
    14941 ttttgccgtc gtgatacgtt ggcttccatc gctggattcc agcatcagcg ccagagactg
    15001 aagtatttcc tcgcgacggt tccttttcgc agtttgtttt tctgccatgt tacaaaaaac
    15061 ccctgaaaat aagcacttgc caggcggcac ccacgctatg accgcaaacg aaatgtttgc
    15121 ggctatgtta tgacgttatt cggatgcgta tgtgttactg acgaccagag tgaccaaagc
    15181 cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag attaaattca gcctgtacta
    15241 ccggaacaaa aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag ctgtcctgac
    15301 cacggttcca cacggaaatc atcaactggc cctgatagtc ggaatcgatc aatcctacca
    15361 ggttaccaag cacgataccg tgcttatgtc ccaatccgga gcgcggcagc atcattgccg
    15421 ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg aaccagcgta gtgtcacccg
    15481 gagccagttc tacggcgtcg tcgagacagg cacgcaggtc aagtccggca gagccagagg
    15541 tggcataagt cgggagcgga aattccttcc caacgcgcgg gtccagaatc ttaacgtcga
    15601 tttttttcat cataacgggt cacgatctcg tcgagtaata attggccaag gagctctttg
    15661 cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc gttgttgtcg
    15721 ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc aaggttttta
    15781 cggatacgtt tttgccgggc gtattcttcc acattatttg tttcggcggc aaatccaacg
    15841 acgtagggtc gatggtcttt tagtgcggca acgcctgcga cgatatcggg gtttttaacc
    15901 atttttattg ttaattcatc accctgcgtg gcctgctttt tgattttctc tggggccacg
    15961 gtagctgcgc gataatccgc cacggcggcg caaccgataa aaatattttg ctgctgtaca
    16021 gaagcattca cggcggcttc catttccagc gcagtcatca catcaacacg tttaacaaac
    16081 ggtggcgtcg gtagtgaaac cggacctgat accagcgtga cgttcgcgcc acgacgggca
    16141 gcggcggcgg cgatagcaaa acccatcttg ccggagctgt gattagagat ataacgcacc
    16201 ggatcgagcg gttcacgcgt cgggccggcg gtaatcataa tgttcagatg tttcaggtcg
    16261 ttgacgggcg aaaaatgcgc caccgccata tccacaatgg ttaacggatc gagcattcgc
    16321 ccaggaccga tatcaccaca agcctgactg ccactgtctg gcccccagat gagcatacca
    16381 cgggaagcaa gcacctctaa attatgctgc gtggcagcgg cacggtacat ctgctggttc
    16441 atggcgggga gcacggctac aggcgcaggt gtagccagac aaatcgtcga taccaggtca
    16501 ttcgccattc cggcagcaat acgggcaatc aaatctgccg tggcaggggc gagaatcact
    16561 aaatcagccc atttacccag ctcaatatgg cccatagcgg cttcggctgc cgggtccagc
    16621 agactgtcgg aaacgggata accagaaacc gcctgcaagc taagtggggt gataaaggct
    16681 tttgccgctt cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa acgacgcacc
    16741 agttcagggg ttttataggc agcaataccg ccgctaacgc cgagaacgat ttttttaccg
    16801 gccaggctca tgatgatttt tcctgtaggg tgacaccaga agttggcgat tttatcacaa
    16861 tacttttgtt gtcgtgcctt cacctgagat tcactttgcg aggcgttttc caggattgaa
    16921 aactggccgt cgatttaacg aaacggctat gacaggatgc gagcaccaca aaggaggtga
    16981 aggtgaaaaa caattcacag ctgttgatgc cgcgcgaaaa aatgctgaag tttggtatta
    17041 gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg taccggaacg cgcggtaaag
    17101 atgtattaac cctggcaaaa gagatgctgg agaatttcgg ctctctttat ggcttgttaa
    17161 cctctgaata tgaacaattt agtggcgttc atggaattgg cgtggcgaaa tttgcccagt
    17221 taaaggggat tgatgaactg gcgcggcgtt actacaacgt gcggatgcgt gaagaaagcc
    17281 ctttactcag cccggagatg acgcgggaat ttttacaaag ccagctcacg ggtgaggagc
    17341 gggagatctt tatggtgatc tttctcgact cccaacaccg ggttataacg catagccgtc
    17401 ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc cgcgaagcga
    17461 taaaaataaa cgcctcggcg ctgatccttg cacataatca cccttcgggt tgtgctgaac
    17521 ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag ttcatggatt
    17581 tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt gccgaacgcg
    17641 gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg gacttgagca
    17701 catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg catttgggcc
    17761 tggcaatcga gagttcacat agaactgcga tgaccgggct gtaaagcctg acgaggcgcc
    17821 aataccccat acgaagctcg agctaatttg atttttggag aatagacatg tcccgagtct
    17881 gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca ctgaacgcga
    17941 ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag agcgagaagc
    18001 gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa aaaggcatcg
    18061 atacagttct ggctgaactg tgtgcccgtg gcgaaaagta ctaagtactt agaggaaata
    18121 aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg gtactggtca
    18181 cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac tgaaaaaatt
    18241 cgatccagtt gttcgccagc acgtgatcta caaagaagcg aaaatcaaat aattctcgct
    18301 ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt tatctgcttg cccccatatt
    18361 gactgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt gaaaccagcc
    18421 gccgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcggtg gtgcgcaacg
    18481 gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa ccagtgctta
    18541 gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc tgagggctgg attatcattc
    18601 atttggggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct gaaaagcatg
    18661 accatgtgga tttggtgatg agcaacggca aagtgctgcg ctacaccgat ccgcgccgct
    18721 ttggtgcctg gctgtggacc aaagagctgg aagggcacaa cgtgctggct catcttggac
    18781 cggagccgct tagcgacgat ttcaatggtg agtatctgca tcagaagtgc gcgaagaaaa
    18841 aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggggta gggaatatct
    18901 atgccagcga atcactgttt gcggcgggga tccatccgga tcggctggcg tcatcactgt
    18961 cgctggcaga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg cgttcgattg
    19021 agcagggtgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg ggctatttcg
    19081 ctcaggaatt gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc ggtacgccga
    19141 ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc cagaagtaat
    19201 tcatgcgcgc cggatggcat accatccggc ataaacgcta cgctaacttc gccatcagcg
    19261 cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgt gccacctctt
    19321 tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac acactttcca
    19381 gttccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca aaatccgcca
    19441 ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag ttcgccatca
    19501 aatcactaaa cccgaccact tccacgttcc ccagatgcgc ggttgcctgc tgtgccagtg
    19561 ccacacgctc ttccagggta aacatcggtt ttttactggg gctggcggca atcgccagaa
    19621 taacgtgatc gaacatctgc gtggcgcgcg tcacgatatc gatatgacca ttggtaatgg
    19681 gatcgaaagt acccggataa atcgcccgtt tttgcataac aacctcaatg cgttttcggt
    19741 ggcaggtaag gttccagcag ttgaagcaga cgctgtagcg cgccctggtt ttgatacagt
    19801 acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag taaagaggaa
    19861 acctcttttg caagcgtagt ggcatcggta acggtaataa gcccgcttgc ctgctccagc
    19921 cgcgcgcaaa tgtctttaaa gttaaaagta tgcggcccca tcaataccgg aatagcgtgt
    19981 gcggcagctt ccagcggatt atgcccacca cgttcaacca gtgaaccgcc aacaaaggcg
    20041 agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac cacaacctgc
    20101 gtgctggtgg aggggacttc ccctgaagag cgtgtgatat agcttagtcc agcctggcgg
    20161 acaaggttaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat gagcaataaa
    20221 ttcgggaatt gctgtaacaa tgcctgatgt gcggcaatga ccacgctctc ttcaccttcg
    20281 tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc attggcggcg tagcgtcacg
    20341 gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc ggtaacggtt
    20401 acctggttat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc attttgcgca
    20461 gcaatcagcg taatacgacg cagcaagcgg cggacgaatt tacccagttt ggcataacct
    20521 gcggccgagc gggcagagag tcgcgcgtta gcgatcacca gcggaatttt acgtttatgt
    20581 agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag tttggggtcg
    20641 actttattca ggaaacggtt gagcgcatcg ggcaggtcat acggcagata aacgtgctga
    20701 acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat ggttgtaacg
    20761 gtaatcggta aatcaggata acgatgacgc agcgcgcgca ccaacgggat cgccgccaga
    20821 gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcgg atggcggtaa
    20881 aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc gcgcacccag
    20941 agccgtatcc agatcagcgg ctgaataagg tagagaaggg cggtgtaaag caattcgagc
    21001 atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct gtggctttac
    21061 cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt gctgaacatc
    21121 tatacttttt ttgtattttt atgcgattca ttgatttctg ctgtacgtgc aggaaggaat
    21181 cgcagagcaa cagtgaaaga atatggttgc taatgatttt atttgattga tttctttatt
    21241 gaaaatcaac aatgtgctta aaaatcattc gggtcggatt gccgcgaaag aagtgataca
    21301 ctagcgcgtc aatatcagtg cggtatcagt attcaggtag ctgttgagcc tggggcggta
    21361 gcgtgctttt tctgcttcac cttaatcgga taatctcaac aaaaagagtt acttgtggat
    21421 aagccatttc gaagaatttt gctcattaag atgcgttttc atggggatat gttattaact
    21481 actcccgtca ttagttcgct gaaaaaaaat taccctgacg caaaaatcga tgtgctgctt
    21541 tatcaggaca ccatcccgat cctgtctgaa aatccagaga ttaacgcgct ctacggcata
    21601 aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact tttttcatct catcaaggta
    21661 ttacgtgcca ataagtatga ccttatcgtc aatctcaccg atcaatggat ggttgctata
    21721 ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg attatcatca tcggcagtct
    21781 gctttttggc gtaaaagttt cacccatttg gtgccgttgc agggtggaaa tgtggtggaa
    21841 agtaacttat ccgtgctgac cccattggga gttgattcgt tggtgaagca gacaaccatg
    21901 agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcacgc tggtgttgga
    21961 caaaattatg tggttatcca acctacggcg cggcaaatct tcaaatgctg ggacaacgcc
    22021 aagttttccg ctgtgattga tgccttacat gctcgtggtt atgaagttgt tctgacgtcc
    22081 ggcccagata aagacgatct ggcctgcgtc aatgaaattg cgcagggatg ccagacgcca
    22141 ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt aatcgatcat
    22201 gcgcagctgt ttattggcgt tgattccgca ccggcgcata ttgccgctgc agttaatacg
    22261 ccgctgatat cgctgtttgg tgcgacagac catattttct ggcgtccctg gtcaaataac
    22321 atgattcaat tctgggcggg agattaccgg gaaatgccaa cgcgcgatca gcgtgaccga
    22381 aatgagatgt atctttcggt tattccggcg gcagatgtca ttgctgctgt cgataaatta
    22441 ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc tttttgttta tataaatatt
    22501 ttccctttgg cggtttgcag cgtgatttta tgcgtattgc tcagacagtc gccgcccgag
    22561 gtcatcatgt tcgggtttat acccagtcgt gggaaggcga atgccctgat gtatttgaac
    22621 tgatcaaagt gccggttaaa tcgcatacca atcacgggcg caatgcggag tattttgcct
    22681 gggtgcaaaa acatttacgc gaacatcccg tcgataaagt cgttggattc aacaaaatgc
    22741 cggggctgga cgtttattat gccgctgatg tttgttatgc cgagaaagta gcgcaggaaa
    22801 aaggcttttt ctatcgcctg acgtcacgtt atcgccatta tgccgccttt gagcgggcaa
    22861 ccttcgaaca gggtaagccg acacagttgc tgatgctgac agataagcaa atcgccgatt
    22921 tccagaaaca ttatcagact gaagcggagc gttttcatat tctgccaccg gggatttatc
    22981 ctgatcgtaa atatagccag cagccagcca atagccgtga aatcttccgt aagaagaatg
    23041 gaataaccga acaacaatat ttattgttgc aggtcggttc agacttcacg cgtaaaggtg
    23101 tcgatcgttc cattgaagca cttgcttcgt taccggattc gctgcgccac aacacattgc
    23161 tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc gctggcagaa aaacgcggcg
    23221 tgcgcagtaa tgttcacttt ttctcggggc gcaatgatgt ctcggaatta atggcggcgg
    23281 cggatttatt actgcatcct gcctaccagg aagcggcggg aattgtgctg ctggaagcga
    23341 ttactgcagg attaccggta ctaacaacag ccgtttgtgg ctatgcgcat tatattgtcg
    23401 acgctaattg cggcgaggct attgctgagc cattccgcca ggaaacattg aatgagattt
    23461 tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat gcgcgacatt
    23521 atgctgatac acaagattta tacagtctgc cagagaaagc ggcggatatc ataacgggtg
    23581 gtctggatgg ttgaacttaa agagccgttt gccacgttat ggcgcggcaa agatcctttt
    23641 gaggaagtta aaaccttgca gggtgaggta tttcgtgaac tggaaactcg ccgtactctg
    23701 cgctttgaaa tggcgggcaa aagctatttt ctcaaatggc atcgcggcac gaccctgaaa
    23761 gagataatca aaaatttact ctcattgcgg atgccagtat taggcgctga ccgcgaatgg
    23821 aatgcgattc atcgactgcg ggatgtcggc gttgatacta tgtatggggt ggcatttggc
    23881 gaaaaaggca tgaatccgct gaccagaact tcatttatta ttaccgaaga tctgacacca
    23941 accataagtc ttgaagatta ctgtgctgac tgggcgacta accctccaga tgttcgcgta
    24001 aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc tgcgggcatt
    24061 aaccaccgtg actgttatat ctgtcatttc ctgctgcact tgcctttttc cggtaaggaa
    24121 gaggagttaa aaatttcggt aattgacctg caccgggcgc agcttcgcac gcgcgttcca
    24181 cgtcgttggc gggataaaga tcttattggg ctttattttt cttcgatgaa tatcggcctg
    24241 actcagcggg atatctggcg gtttatgaaa gtgtattttg ccgccccgct taaagacatt
    24301 ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa aatcagggaa
    24361 agaacgattc gaaaatcgtt gtaacccgta acttattttt gccaaaattt tggatacaga
    24421 ataaatatgt ctcaactcaa tgatagtgac atcatccttt ttgagtataa ttttcattat
    24481 caaaat
//
LOCUS       DAZFAH010000052        24005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFAH010000052 DAZFAH010000000
VERSION     DAZFAH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.052"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..145
                     /locus_tag="ACHJWB_001989"
     CDS             <1..145
                     /locus_tag="ACHJWB_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4313633.1"
                     /translation="IASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQID
                     MQF"
     gene            178..339
                     /locus_tag="ACHJWB_001990"
     CDS             178..339
                     /locus_tag="ACHJWB_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313634.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            326..1147
                     /locus_tag="ACHJWB_001991"
     CDS             326..1147
                     /locus_tag="ACHJWB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4313635.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            1144..2520
                     /locus_tag="ACHJWB_001992"
     CDS             1144..2520
                     /locus_tag="ACHJWB_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4313636.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDVITGGMNSEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVAKGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDESSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTTSNAPAARGR
                     RYAQGADV"
     gene            2593..2799
                     /locus_tag="ACHJWB_001993"
     CDS             2593..2799
                     /locus_tag="ACHJWB_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313637.1"
                     /translation="MAELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVIFG
                     SILLWVIIGVAPVAVAKMAWRFVS"
     gene            2817..3131
                     /locus_tag="ACHJWB_001994"
     CDS             2817..3131
                     /locus_tag="ACHJWB_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313638.1"
                     /translation="MDESRKQFEESWLRRGGESSDLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIEIELQKPKKGPLPGDYHIGYDSGAESQYESDVEAIRAAGIKV
                     KE"
     gene            3331..3741
                     /locus_tag="ACHJWB_001995"
     CDS             3331..3741
                     /locus_tag="ACHJWB_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4313639.1"
                     /translation="MKQTIFLRTKQQQQAAINAILETPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            3738..3914
                     /locus_tag="ACHJWB_001996"
     CDS             3738..3914
                     /locus_tag="ACHJWB_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4313640.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRTRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            3917..4300
                     /locus_tag="ACHJWB_001997"
     CDS             3917..4300
                     /locus_tag="ACHJWB_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HFO4313641.1"
                     /translation="MDYSQLSDFEINVAVFEAIHNGSPDYKEGENGDMVFVSFEGDIV
                     NGDAVEVEVERGSFNPCVNPADAWPIITENNISIILDNPSMPCATDNARDLFDDAGPN
                     VGVAYDNPLRAAMIVFLMLQDANNA"
     gene            4293..4469
                     /locus_tag="ACHJWB_001998"
     CDS             4293..4469
                     /locus_tag="ACHJWB_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4313642.1"
                     /translation="MLSPSQSIQYQKESVERALTCANCGKKLHVLEVHVCSDCCVELM
                     SDSNSSMYEEEDDE"
     gene            4462..5184
                     /locus_tag="ACHJWB_001999"
     CDS             4462..5184
                     /locus_tag="ACHJWB_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4313643.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEYYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAISAPKVEFADRVGEASGILIGNYAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            5184..5474
                     /locus_tag="ACHJWB_002000"
     CDS             5184..5474
                     /locus_tag="ACHJWB_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4313644.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            5471..5833
                     /locus_tag="ACHJWB_002001"
     CDS             5471..5833
                     /locus_tag="ACHJWB_002001"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4313645.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            5830..6018
                     /locus_tag="ACHJWB_002002"
     CDS             5830..6018
                     /locus_tag="ACHJWB_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO4313646.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            6015..6533
                     /locus_tag="ACHJWB_002003"
     CDS             6015..6533
                     /locus_tag="ACHJWB_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4313647.1"
                     /translation="MSIRELNLTKEQHDWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLAI"
     gene            6597..6671
                     /locus_tag="ACHJWB_002004"
     tRNA            6597..6671
                     /locus_tag="ACHJWB_002004"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6630..6632,aa:Asn,seq:gtt)
     gene            6677..6752
                     /locus_tag="ACHJWB_002005"
     tRNA            6677..6752
                     /locus_tag="ACHJWB_002005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6710..6712,aa:Thr,seq:tgt)
     gene            6995..7318
                     /locus_tag="ACHJWB_002006"
     CDS             6995..7318
                     /locus_tag="ACHJWB_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO4313648.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            7302..7778
                     /locus_tag="ACHJWB_002007"
     CDS             7302..7778
                     /locus_tag="ACHJWB_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4313649.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            7775..8212
                     /locus_tag="ACHJWB_002008"
     CDS             7775..8212
                     /locus_tag="ACHJWB_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4313650.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLR"
     gene            8200..8352
                     /locus_tag="ACHJWB_002009"
     CDS             8200..8352
                     /locus_tag="ACHJWB_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313651.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLEVEHLA
                     RGKGLR"
     gene            8555..9073
                     /locus_tag="ACHJWB_002010"
     CDS             8555..9073
                     /locus_tag="ACHJWB_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4313652.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIERLE
                     QQAQISIPGLPK"
     gene            9427..9669
                     /locus_tag="ACHJWB_002011"
     CDS             9427..9669
                     /locus_tag="ACHJWB_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO4313653.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            9672..10112
                     /locus_tag="ACHJWB_002012"
     CDS             9672..10112
                     /locus_tag="ACHJWB_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4313654.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPKFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            10109..11524
                     /locus_tag="ACHJWB_002013"
     CDS             10109..11524
                     /locus_tag="ACHJWB_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO4313655.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADGVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            11526..13724
                     /locus_tag="ACHJWB_002014"
     CDS             11526..13724
                     /locus_tag="ACHJWB_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO4313656.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMRKQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPIVDVDFIPGPLMNHWAERNKKRPAVLPMTSKKD
                     KNGTVISEAQVAGWTPQTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            13815..14708
                     /locus_tag="ACHJWB_002015"
     CDS             13815..14708
                     /locus_tag="ACHJWB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFO4313657.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQEQIRERKREEEAR
                     TATLQQKAANYMHRVKALKVAGYQDAEQAVREDVPVHIQDMILLESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KARALETGDWTPYFAAKKAKK"
     gene            14727..15980
                     /locus_tag="ACHJWB_002016"
     CDS             14727..15980
                     /locus_tag="ACHJWB_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO4313658.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSSKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMSDSTTGTQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RLPKGSAESLTVSGQPEHKVEAKDSNGMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPVENADVASRPYANVDAKPAESAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            16022..16210
                     /locus_tag="ACHJWB_002017"
     CDS             16022..16210
                     /locus_tag="ACHJWB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313659.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            16191..16652
                     /locus_tag="ACHJWB_002018"
     CDS             16191..16652
                     /locus_tag="ACHJWB_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO4313660.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDQST"
     gene            16662..18080
                     /locus_tag="ACHJWB_002019"
     CDS             16662..18080
                     /locus_tag="ACHJWB_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO4313661.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFRLRVITKSPVTLSGCQIRIE"
     gene            18080..18928
                     /locus_tag="ACHJWB_002020"
     CDS             18080..18928
                     /locus_tag="ACHJWB_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO4313662.1"
                     /translation="MADSNLNTPVIMQATRLDTSILPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQDHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            18928..19407
                     /locus_tag="ACHJWB_002021"
     CDS             18928..19407
                     /locus_tag="ACHJWB_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO4313663.1"
                     /translation="MEIKLIDNPVKLAEFLNNPANTGNIVDSGDKYYIKPDAVYLGIY
                     EGLMLVGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRSIIRLLGATRVGHLDDAYTSNGKPVGITIYQLPRSKYEELKNVNFPDCQ"
     gene            19382..20056
                     /locus_tag="ACHJWB_002022"
     CDS             19382..20056
                     /locus_tag="ACHJWB_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4313664.1"
                     /translation="MLIFQIANKHLSKAVYCKGGSDGGSKAQARATEKGIELQREMWQ
                     TNMQNLAPFTPLAQQYVSELQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAAAE
                     ATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYANNV
                     SQLYQQQAAASAANANRPSGLQSALGGAMSGAASGAMIGSVVPGIGTAVGAIGGGIIG
                     GLGSLF"
     gene            20066..20743
                     /locus_tag="ACHJWB_002023"
                     /pseudo
     CDS             20066..20743
                     /locus_tag="ACHJWB_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            21538..23376
                     /locus_tag="ACHJWB_002024"
     CDS             21538..23376
                     /locus_tag="ACHJWB_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4313665.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGIGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDSMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILANDATDR"
     gene            complement(23398..>24005)
                     /locus_tag="ACHJWB_002025"
     CDS             complement(23398..>24005)
                     /locus_tag="ACHJWB_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313666.1"
                     /translation="AYVIGSLGFLIFFVWQYQSLSPGWRFLGMSLISLGAGIATQVLM
                     YLFNGWLSKRVEKKRATSICRSLAIPEDSTDQDDIAKCWRYMIARYSNELLANRLSDL
                     IGIVVTSVGTIISIGISIWYVGMIVYFVWNRDFNEPSLLFIPLFFRVLAFICELLLSF
                     FCNVLFNRYPGEARKFNKNYDELRRTDPFLSSKEFRDSIRN"
BASE COUNT         6817 a         5568 c         6180 g         5440 t
ORIGIN      
        1 tattgcttcg gtcttgtgtg ctttcggaat ggcatcagac atcagtccga ttagcagggc
       61 ttttaagtat gcgcttgatg aaatcacaaa gaaaaaatcc ccggccgcca ccgaggattt
      121 taagcaaatt gatatgcaat tctgagggaa ttactggatc aatccacagg agtaattatg
      181 acaaaacgtc gtaagaaata ccaggaaaaa gaagagattc gacaccctga ttcacctgag
      241 ggattagtgg tagccgcagc aaataacagg gcgttcgcag agcgccttgt tggtgtttac
      301 agactagcca aagcaggagt gaaacatggg cgtcgttaag ttagctgatt acaggcctca
      361 actggaggtc gtggagcatc gcgtggcaga taccgaagat ggtttcatgc gcgttgctaa
      421 cgagattacc gacagtctgc tgatggctga tttaaccgtc cggcagttga aggtgatgct
      481 cgctatcatg cgcaagacat acggattcaa taagccgatg gatcgactca caaacacgca
      541 gatagcagcc atgacaggta ttcatcacac tcatgtttgc gctgccaagc gccagcttat
      601 tgagcgtaaa ttcctcattg ctgatggcgt gaaaatcgga gtgaacaagg tggtttctca
      661 gtggattagc caggacagct taacattagc taaaacagct aataaaacat tagccaagtc
      721 ggctaatggg tataagccaa gtcagctaaa cacaaaagac aatatacaaa agacaataaa
      781 tacaaatacc cccttacccc ctaacggggg cggcgatggg caggttaaac ctgaacgtcg
      841 caaggcagaa cgaatcgact acgaatcctt cctgaacgcc tacaacaccg aagtcggtga
      901 cagactgcca cacgctgttg cggtcaacga gaaacgcaaa cgccgcctga agaaaatcat
      961 cccgcaactg aaaacgccaa acgtggacgg tttcagagcg tatgtcaggg cgtttgtaca
     1021 tcaggccaag ccgttttact tcggagacaa cgacacgggc tggacggctg attttgatta
     1081 cttgctgaga gaagattcgt taacgggagt tcgggaaggg aagtttgcag acagggggat
     1141 tgcatgagac aggatatcga agcgagcgtt atcggtggcc tgctgattgg tggattaaca
     1201 ccaaccgcca gtgacgttct ggcaacgctt gagccggaag cgttttcaat tccgctctac
     1261 cggaaagcct tcgaggttat ccgcaagcag gcgcgaaaca gaaacctaat cgacgcgctg
     1321 atggttgccg aggcgtgcgg agaggagcat ttcacgtcaa tcctgatgac cagcaaaaac
     1381 tgcccgagtg ccgcaaacct gaagggatat gccggaatgg tcgcggataa ctatcaccgc
     1441 cgtctggtgc tggaaatcat ggatgaaatg cgtgaaccaa ttcagagcgg aaccatcgac
     1501 gcatcgagtc aggcgatgga tgaacttgta aagcgtcttt cagccatcag aaagccccgt
     1561 gacgaggtta aacctgtacg gttaggggaa atcatcactg actacactga cacgcttgac
     1621 aggcgtctga ggaacggaga agagtcagat accctgaaga ccggaatcga agaacttgat
     1681 gtcatcaccg gagggatgaa ctcggaagac ctggtgatta tcgctgctcg tccgggtatg
     1741 gggaaaaccg agctggcgct gaagattgcc gaaggcgttg caagccgcgt tattcctggt
     1801 tctgacgtcc ggcgcggagt gttgattttc tcgatggaaa tgagcgcatt gcagatcgca
     1861 gagcgaagca ttgccaacgc cgggaggatg tcggttagcg tactgcgaaa tcctgcatcg
     1921 atggatgacg agggctgggc acgtgttgct aaaggcatga gtcagcttgc agatttggat
     1981 gtatgggtag tcgatgcctc gcggttatcg gtcgaagaaa ttcgctcaat cgcagaacgg
     2041 cacaaacagg aaaatccaaa cctgtcactc atcatggcag attatcttgg cctgattgag
     2101 aagccgaaag cagaccgcaa cgacctcgca attgctcaca tctccggaag cctgaaggcg
     2161 atggcgaaag acctgaaaac gcctgttatc tccctgagtc agctttcgcg cgatgttgag
     2221 aagcgaccaa acaaacgccc gacaaacgca gatttgcgtg attcaggaag cattgagcag
     2281 gacgcagact caatcatcat gctctatcgg gaagcggtat atgacgagag cagtagcgcc
     2341 gcgccatttg ctgaaatcat cgtgacgaaa aaccgttttg gctcacttgg tacggtttac
     2401 cagcggttct gtaacggaca ctttgttgca tgtgaccagg atgaagccag acagatttgc
     2461 acaacatcaa atgcacccgc tgcacgtggc aggcgatatg cacaaggggc tgacgtatga
     2521 ccatctacat cactgagctt gtaacaggcc tgctggtaat cgcaggcctt tttatttggg
     2581 ggagagtaaa tcgtggctga gttaattttc tctgcattga ggattctcgg tgctatgtgg
     2641 atggtggcga cgttcattgt ggttgccagc agttttgtcc ggttggtagg cgaaggtaaa
     2701 gacctggtgg gtgtgatttt cggtagcatt ctcctgtggg tgattatcgg tgttgcgcct
     2761 gtcgctgtag caaaaatggc gtggcgtttt gtgagttgaa ctgagggtaa gtatcgatgg
     2821 acgaatcaag aaagcagttt gaagaaagtt ggttgcgacg tggaggcgaa tcatcagacc
     2881 ttatccgtta ccctgaaaat caccatgaaa ttggcagtgg tgatattggt ggtcaatacg
     2941 tgatggacga tgttcaaggc cactggcaaa cgtggcaggc atcgagagca gctattgaaa
     3001 ttgagctgca aaagccaaag aaaggcccac ttcccggtga ttatcacatt ggctatgact
     3061 caggtgcaga atcgcaatac gaaagcgatg tagaggctat ccgcgccgct ggaatcaaag
     3121 tgaaggagtg agcatgagtc gacgaagtag ctttttgggg tttgtaatat tcctgtcctg
     3181 cactggttac atcgtaatct ggtcaatttc gaacattgac cgtggcgggg aatatctcat
     3241 tgtaatgttc tttcctttgt ttcttgggtg gtacgccgca aggttgctgg aagaatgggg
     3301 ttacaggcat aaaaaataaa ggagtgttca gtgaagcaaa caatattcct ccgaactaag
     3361 caacaacagc aagccgcaat caacgccatc ctcgaaactc ctctcgataa agacaagcca
     3421 gtcaccatcc gcattactga ctacaagcga aatcttgacc agaacgcaaa atttcacgcg
     3481 atgctggcgg atatcgctcg tcaagttcaa tggtgcggca aatggttaaa accggaacaa
     3541 tggaaggttt tgttgatcag cggtcatgca gtggcaacaa aacaggaagc tgatgttttg
     3601 ccaggccttg aaggcgaata cgtcaacatt cgcgaaagca gcgcgcagat gagcgtgaag
     3661 cgtatggcaa gtctgatcga gtacacaaca gcctgggcta ttggtcaggg tgtcagattt
     3721 accgacagga ggtacgaatg agacgacagc gacgaagtat caccgacatc atctgcgaaa
     3781 actgcaaata ccttccaacg aaacgcacca gaaataaacg caagccaatc ccaaaagaat
     3841 ctgacgtaaa aaccttcaac tacacggctc acctgtggga tatccggtgg ctaagacatc
     3901 gtgcgaggaa atgacaatgg attattcaca gttaagtgat tttgaaatta acgtggcggt
     3961 attcgaagcc attcataacg gatcaccgga ttacaaagaa ggtgagaatg gcgatatggt
     4021 gtttgtctca tttgagggag acattgtaaa cggagacgca gttgaagtag aagttgagcg
     4081 cggatcattt aacccatgcg taaacccagc agacgcatgg ccgattatca ctgaaaacaa
     4141 catcagcata attttagaca atccctcaat gccgtgcgcc acagacaacg caagggactt
     4201 gtttgatgat gcaggaccga atgttggtgt cgcatatgac aatccactcc gtgccgccat
     4261 gattgtcttt ctcatgctgc aggacgccaa taatgcttag cccatcccaa tccattcaat
     4321 accagaaaga aagcgtcgag cgagctttaa cgtgcgctaa ctgcggtaag aagctgcatg
     4381 tgctggaagt tcacgtgtgc tccgattgct gtgtagagct gatgagcgat tcgaatagct
     4441 cgatgtacga ggaagaagac gatgaatgag ttaataaatg gcaatgccat caaaatgaca
     4501 agcattgaaa tcgctgagtt ggttggtaag cgtcatgaca atgtgaaacg taccatcgaa
     4561 acgctggcta aaaatggtgt tatccggctt cctcaaattg aggtttccga aagaatcaat
     4621 aacttagggt tcaatgttca gtacgagtat tacgtcttcg aaggcgaaca aggaaagcga
     4681 gacagtattg tcgttgtagc ccagttgtcg ccggaattca ccgctcgtct tgttgaccgc
     4741 tggcgagagc ttgaagaaac tgcggttaat atccccaaaa cgttaccgga agcgttgcgc
     4801 cttgctgctg atcttgctga gcagaaaatg caactggaaa accagctcgc aatttccgca
     4861 cctaaagttg agtttgccga tcgagttggc gaggccagcg gaattttgat tggaaactat
     4921 gcaaaggttg ttggaattgg tccaaacaaa ctgtttgcgt ggatgcgcga tcacaaaatc
     4981 cttattgctt caggttcccg gcgcaatgtg ccaatgcagg aatatatgga tcgcggctat
     5041 ttcacagtga aagaaacagc ggtcaacaca aatcacggaa tacagatatc gttcaccaca
     5101 aaaatcaccg ggcgtggtca acagtggctg accagaaagc tgctcgataa cggaatgctg
     5161 aaagtaacag gtgaggctgc ttaatggcta atctacgcaa agaagcacgc ggcagagaat
     5221 gccaggtacg tatttacggc gtatgcaatg gcaatcctga aactacagtt ctggcacatt
     5281 accggatggc tggaatttgc ggaacgggaa tgaagcctga cgacctgatc ggcgcatggg
     5341 cttgtagcgc gtgtcacgat gagatcgacc gacgcaccca taacctcgac aacaaagacg
     5401 ccagacttta ccacctcgaa ggcgtaatca ggacgcaggc gatactgctg aaggagggga
     5461 agattaagtc atgaacgaat atcagtttgt gcttccatac ccgccgtcgg tgaataccta
     5521 ctggcgaaga cggggaagcc aatactacat cagcgataaa ggccagaaat accgaaaaga
     5581 cgttcagcaa ataatccgcc aactcaagtt agacattttc accaaatcac gactccgcat
     5641 caaagtcatc gcagacgttc cagactcccg ccgccgcgac ctcgataaca tcctgaaagg
     5701 tttactcgac tcccttatcc acgccggatt tgcggaagac gacgagcaat tcgatgacat
     5761 tcgcgtaatt cgtggcgtga aagtaccagg cggaaggctt ggaataaaaa tcaccgaact
     5821 ggagaacgca tgaacgccac aattcaaacg ataccagagc ttcttatcca gacacgaggc
     5881 aatcagaccg aagtggcaag gatgctttcc tgcgcaagag gaacagtgct caagtacaac
     5941 cgagacagca aaggcgagcg tcacgtaata gttaacggcg tcctgatggt caaacagggc
     6001 aagaggggaa gacgatgagc ataagagaac taaacctcac caaagaacag cacgattggc
     6061 tgaatggctg gcttgaactg tggggcgcat gggtttattc aggccgcctg gaaaagcgca
     6121 tgagcagcgt aatagcgaag ttcatggaga gcgtagagcc gggaagagtt atgacaaggc
     6181 caatgtgtaa tgatgatgat ggaatgttga tttctcaggt cgtcgattcc gtcatgtaca
     6241 ttgacaagaa agcttttggc atcctcctca gctactacgc tcatggttca tctaagcgag
     6301 caattgcatc ctactatcac gcgactgcaa agccacgcaa gatgtgtgga cgtggtggcg
     6361 agggatggag aaaaccttca ctggcaacct gtagaaacga aattgacgac atcctgaaag
     6421 cgtcattatt tgttttatac caaccaatgc aaaatgcttt caaaatgcgt aaacgtgttg
     6481 agaaagttaa gcatgttgct gttaaaagtc ttgacatgca attagccatt tagccataat
     6541 tagaaggtaa gctgccgtta gtaactctta agttgcaacg gtggcttttt ttatttgggt
     6601 cagtcgtata aaggtcatta cggaaggctg ttaaccttct tatcgtggtt cgagtccacg
     6661 ctgtcccgcc aaatatgctg gtttagctcc aatggtagag cagtcgcctt gtaagcgaat
     6721 ggatagcggt tcaagtccgt taaccagcac cataactgag ccgtagccac tgactatcct
     6781 gaattcatca gtgatagtta cgctgcggcc ttctacacat gaccttcgtg aaagcgggtg
     6841 acaagaggtt gcgctaacaa cctcctgccg ttttgcccgt gcatatcggt cacgaacaaa
     6901 tctgattact aaacacagta gcctggattt gttctatcag taatcgacct tattcctaat
     6961 taaatagagc aaatcccctt attgggggta agacatgaag atgccagaaa aacatgacct
     7021 gttagccgcc attctcgcgg caaaggaaca aggcatcggg gcaatccttg cgtttgcaat
     7081 ggcgtacctt cgcggcagat ataatggcgg tgcgtttaca aaaacagtaa tcgacgcaac
     7141 gatgtgcgcc attatcgcct ggttcattcg tgaccttctc gacttcgccg gactaagtag
     7201 caatctcgct tatataacga gcgtgtttat cggctacatc ggtactgact cgattggttc
     7261 gcttatcaaa cgcttcgctg ctaaaaaagc cggagtagaa gatggtggaa atcaataatc
     7321 aacgtaaggc gttcctcgat atgctggcgt ggtcagaggg aacagataac ggacgacaga
     7381 aaaccagaaa tcatggttat gacgtcattg taggcggaga gctattcact gattactccg
     7441 atcaccctcg caaacttgtc acgctaaacc ccaaactcaa atcaacagca gccggacgtt
     7501 accagcttct ttcccgttgg tgggatgcct atcgtaagca gcttggactg aaagacttct
     7561 ctccgaaaag ccaggacgct gtggcactgc aacagattaa agagcgtggc gctttaccga
     7621 tgattgatcg cggtgatatt cgtcaggcta tcgaccgttg cagcaatatc tgggcttcac
     7681 tgccgggcgc tggttatggt cagttcgagc ataaggctga cagcctgatt gcaaaattca
     7741 aagaagcagg cggaacggtc agagagattg aggtatgagc agagtaaccg cgattatttc
     7801 cgctctggtt atctgcatca tcgtctgcct gtcatgggct gttaatcatt accgtgataa
     7861 cgccattacc tacaaagccc agcgcgacaa agccgcatcc actatcgctg acatgcagaa
     7921 gcgtcaacgt gatgtagcag aactcgacgc cagatacaca aaggagcttg ctgatgctaa
     7981 cgcgactatc gaaagtctcc gtgctgatgt ttctgctggt cgtaagcgcc tgcaagtcgc
     8041 cgccacctgt gcaaagtcaa cgaccggagc cagcagcatg ggcgatggag aaagcccaag
     8101 acttacagca gatgctgaac tcaattatta ccgtctccga agtggaatcg acaagataac
     8161 cgcgcaggtt aactacctgc aggaatacat caggacgcaa tgccttcgat gatagcgata
     8221 attttactca tcatccttca catctggctc tgtagacagg gtggtgatca cttctggagt
     8281 gaatccagat taaacatctc attgctgatg cttgaagttg agcatctggc gcgcggtaag
     8341 gggctgcgtt gagataagag ccagttcatt acaaatacca ggatttagcc tcgcattcgc
     8401 ggggcttttt atatctgaat ttcacagcgc atctcacgcg catattaacg agagcctttc
     8461 agtaagcgag cctgagaaat gccgttatag gtggcgacct ctctcgggcg gcttttctgt
     8521 gagacaggct cactttctaa aaggtaaaga cgctatgaat catcaattgg ctaatctcga
     8581 tttccgggac atggtggttg tttctggtga tcgcgtgatc acaacctccc gcaaggtagc
     8641 agcttacttc gacaagcagc atcaccacat cattcagaaa atcgaaaagc tagactgttc
     8701 ggatgaattt ctaaccagca acttttcgcg ggttacctat gaacacaagg gtaatcagta
     8761 tgttgaatat gaaatttcca aagacggcgc gatgtacatc atcatgtcgt ttaccggcaa
     8821 aaaagctgcc gccatcaaag aggcgtttat caaagcattt aattggatgc gtgacaggct
     8881 gatggagatg gctcactcat accaaagaga gcacaacgag ttaatgctgg agttcatgaa
     8941 ggaaaaggat gttgccagta tgtcaggacg cttgctgaac cgctggggca ggatcaaaaa
     9001 accgcaactc atagcaagaa tcgaaaggct tgagcagcag gcacaaatat cgatccccgg
     9061 actgccaaag tgaccattcc aaagcccatc tacgggtggg cttgataatg aaaccgtgat
     9121 ttacattccc acaatccggg tatgtaaaag atagttcagg cgagaacaga tttaactaaa
     9181 tctgtgcacc accagttaac ggcagtacag cgaaacaacc caagccagta agtggggaaa
     9241 taacactggc agccactgaa agatgaacct cctgccttat ggcaaaaaag attctttgtg
     9301 gtggcggact gatggaaaga catcggttat tgcagagacc attcaatgag tggtctcgac
     9361 aatggcttat accctacacg ggataactta actgatatcc cttttaacgg ataaacggag
     9421 ccaacaatgg cagagattat tcccatgact gaagaacaga aattccagtt agagatttac
     9481 aaactggtca tgaaccagaa cgcagccgca gaggaagcat ttcaattcat cggcactgac
     9541 gagctgaagc ttgagctatt caaaattcac ttccagtcag gcggcgctaa ttcagatatc
     9601 acgactcgca ctatcgaagc ggtgcgtaaa tcgaaggaag cgttagacct gttcactacc
     9661 ggagcataaa catggcgcgc ccaacaaagt atcaagaggc gtatgccgaa caggcacgca
     9721 aactgtgctt gctgggctac accgatgcag aacttgctga tttctttgaa gtcagtgagt
     9781 caactattaa caagtggaag cttgattatc ctaagttttc ggagtccata aaaaagggta
     9841 aggccgtcgc tgatgcagaa gttagtgacc gtctttatca acgcgctatg ggcttcgtgg
     9901 ctccagacat cgatattcgt gttattgaaa acagaattgt cgaaactccg cttgagaagt
     9961 attacccgcc tgatacaacc gctgccatct tctggcttaa gaatcgacag aaggataaat
    10021 ggcgcgacaa ggttgatcac gagctaacag gcaaagacgg cggcgcaatc cagattgaaa
    10081 catcaccgat gagcactcta ttcggaaaat gacctcgatt aatcctatct ttgaaccgtt
    10141 cattgaggcg catcgctaca aagtcgccaa aggcggtcga ggtagcggta agtcatgggc
    10201 aattgcgagg ctgcttgttg aagcggcgcg tcggcagcca gtgcgtattc tctgcgctcg
    10261 tgaactgcaa aacagtatca gcgattcggt aatccggttg cttgaagata ccatcgagcg
    10321 tgaagggtat tcggctgagt ttgaaattca gcgttccatg attcgtcatc tcggaacgaa
    10381 tgctgagttc atgttctacg gcatcaaaaa caaccctacg aagattaaat cgctcgaagg
    10441 cattgatatc tgctgggtgg aagaagcgga agcggtaaca aaggaatcat gggacatcct
    10501 gataccaacc atccgcaagc cgttttccga aatatgggtg agcttcaacc cgaaaaacat
    10561 cctcgacgat acctatcagc ggttcgtcgt aaatcctcct gatgatattt gcctgctgac
    10621 ggtgaactac accgacaacc cgcattttcc tgaagttctc cgtctggaga tggaagagtg
    10681 taaacgcaga aatccgacac tgtatcgtca catctggctt ggtgagccag tgagcgcaag
    10741 tgatatggca atcatcaaac gtgaatggct tgaagctgca accgatgcgc acaagaaact
    10801 cggatggaaa gcgaaaggtg cggttgtctc tgcgcatgac ccgtcagata cagggccgga
    10861 tgctaaaggt tatgcatcgc gtcacggttc ggtagttaag cgcattgccg aagggctgct
    10921 gatggacatc aacgaggggg ctgactgggc aacttcgctg gcgattgaag acggcgctga
    10981 ccattacctg tgggatggag atggtgtcgg tgcagggcta cgcagacaga caacggaagc
    11041 gttctccggc aagaaaatca ccgccacgat gttcaagggc agcgaatcgc cattcgatga
    11101 agatgcgcca tatcaggccg gagcatgggc tgatggagtc gtacagggcg acaatgttcg
    11161 cactattggc gatgtattcc gcaataagcg agcgcaattc tattacgcgc tggctgacag
    11221 gctttatctg acatatcggg cggttgttca cggtgagtat gcagacccag acgacatgct
    11281 gagtttcgac aaagaagcga taggcgagaa gatgctggag aagctgtttg cagaactgac
    11341 gcagattcag cgcaaattca ataacaacgg gaagctggag cttatgacta aggtcgaaat
    11401 gaagcagaag ctcggtattc catctcctaa cctggctgat gcgctgatga tgtgtatgca
    11461 ttgcccggag tcggctgcgc aacccgacta ttccagttac tcaattcctt gtggtgtagg
    11521 ttgatatggc agaaaaaaag atgactgact ggcatcgcaa ggtgctgtgc aactttgata
    11581 atgcctggtc agcaacgcag gatatgcgta agcagattat tgaggctcaa cgtttcgtcc
    11641 gggtgtccgg cgcacagtgg gaaggcagca caaacgctgg ttactcattt gatgaaggca
    11701 ggtttgagca ttacccgcgc tttgaactga ataagattgc ccgtgaatgt gatcgcatca
    11761 ttggtgagta tcgacagaat cgcatcagcg ttaagttcag gcctaaggat gacaaggcat
    11821 cggaagcgtt agccgaaaaa atgaacggca aattccgcgc tgactatcag gaaacatccg
    11881 gtggcgaagc gtgtgataac gcatttgatg atgctgtaac gggcggattc ggttgtttcc
    11941 gcatgtgtgc cgattacgaa gatgaaatgg atccgagtaa cgagcagcga cgcatcagtc
    12001 ttcttcctgt ttacgaccca gcgacatgcg tcttcttcga tcaggacagc aagcaatatg
    12061 accgctctga tgctatgtgg gctatggaaa tgttctccat gacgcccaaa gcgttcgagg
    12121 ctgaataccc tgattccatc gcagcaagcc tttctcgtga tgacactggt actcagtatg
    12181 actggtcaac gcccgatgcc atctatgttg gacgctacta cgaagttcgc atagagaagg
    12241 tgaagctcac ggcgtggcgc aaccctgtta gcggagaaac ggcaatctat gatgaagagc
    12301 aaatcaaaga tattgtcgac gagctgaccg atggtgcatt cgaactgatt ggcgagcgaa
    12361 cggtgaagaa gcgccgcgtt tattgcggtc ttctgtctgg cgctgaatgg ctggaagaac
    12421 cgaagcgtat tccgggtgaa catattcctc tcatcccggt atatgggcga cgctcatttg
    12481 ttgataatca ggagcgaatc gaaggccacg ctgcaaaagc gatggatgca cagcgtcttg
    12541 agaacctgat ggtttccatg attgcagata acgctactca ggctggcggt gatggcattc
    12601 ctatcgtgga tgttgatttc attcccggtc cattaatgaa tcactgggca gagaggaata
    12661 agaaaagacc tgcagttctt cccatgacca gcaagaagga caaaaacgga acggtcattt
    12721 cagaggctca ggttgctggc tggacacctc agacacaaat gcctccagct cttgccgggc
    12781 tattgcagta caccggaacg gctattcagc aaattacagg tgcgtcgcag cttgagaaca
    12841 tgccgagcaa cgtcgccacc gataccgttg atagcatttt taaccggatg gacacgcagt
    12901 cctatatcta catggacaac atggctaaat ccatgcgccg cgctggcgtt gtgtggcttt
    12961 ctatggcgcg tgaagtctat ggcagcgata cgccaatgcg catcgttaat gaggacggca
    13021 gcgatgacgt ggcgctgatg actggtgaag tggttgaccg tcagacaggg caggttatcg
    13081 cgcttaacga cctttcgcag ggtaactatg aagtgactgt cgatgtcggt cagtcgttcg
    13141 ctactcgccg tgatgcaacg gttaagtcgt tactttccat gctggcactt atcccaccag
    13201 gaacgccgaa gcacgacctt gtatcgtcga tgattctcga caatatggac ggcgaaggga
    13261 tggacgacct gaaagaatac aaccgcaatc agttgcttct gtctggcgtt atcaagccga
    13321 gaacgcctga agaacagcag atggttgaac aggcgaaaca acaacaggcc agtcagccag
    13381 atccggctat ggttgctgcg caaggtcagc ttcttgctgg tcaggctgaa ttgcagaaag
    13441 cgcagaacga acaggcagcc attcaggtta aagcattcca ggcacagact gatgctcagg
    13501 ttgcagcggc aaacgttgtg aaaatcctcg catctgccga tagccagcag aaatctgata
    13561 tccgcgaggc tctgaaactg ctcggacagt tccagcaaca gcaaggagat aatgcccgtg
    13621 ctgatgcaga gcttgtcctg aaaagtcagg ctcagggcca tgcgcagcgc atggacatca
    13681 gcagcatcct gcaaaaatca actcagcaac aaccacagca gtaattaacc cataacgtgc
    13741 aatggctgtc tttatgaggc ctggcaccct attgccttcc gatgggctga acatcgagta
    13801 aacaggggta acaaatggac cagatggcag aaaacacacc agaagttgaa atcgaaaccg
    13861 acgcgtcaga gcagattcct gatgatgtcg aactggctga agaagtcgaa acagaagatg
    13921 gcagtgagtc ctccggcaat gatgcagagg aagctactga aactgatgac gacgaatcag
    13981 agcaggaatt ctactttggt gacgaaaagc tggattcgcc aaccagcgaa gatggcgcag
    14041 agcatggact ggtaaaacac ttgcgcaaga cgattaaaga gaaagaccgt gagctgaaag
    14101 agctgatgcg tcagtctcag aaacccgtcg agcagcagcc ggtaatcact caaccaccgc
    14161 gaatgccaaa actggatgat gaggacatcg gtttcgatga agaaatctat cagcaacgca
    14221 tggctaagtg ggcagaggat aacggcaagt accaggagca aatacgagag cggaaacgag
    14281 aggaagaggc gcgtaccgca acgcttcagc agaaagcagc caattacatg catagagtaa
    14341 aagcactgaa agtggctggc taccaagatg cagagcaggc tgtacgcgaa gatgttcctg
    14401 ttcacattca ggacatgatc cttcttgagt cagagaagcc ggaaatcgtt gttctggcac
    14461 tcggccgcaa cgctgaactg cgcaagcaac tggcagaagc tacaaacccc gtagcaattg
    14521 gtcgtctgct ggaacgtatc gaatcgaagg ccagaatcat gccaaaagca aaaaccacgg
    14581 cagccacaac cccgacagtt aaggggagca acggcgcagt aatcaacaac ctcgacaaat
    14641 tgaaagccag ggcgctggaa actggtgact ggacgccgta tttcgccgct aaaaaggcaa
    14701 aaaaataacc tatcggagca ttaagcatgg ctaaccaatt agcaaaagac cttgaaatca
    14761 tgttcgaaaa ctacgttgaa ggctttgagg ccgcctgcgt agtttcccgt aacgctaaaa
    14821 aattccgtcc cggtgataca gcaatgcagc gagcaggtga tgttctgtat cgtccgcagc
    14881 attaccacat gaacattgag gaaggcctcg acctcagcag caaaacgcca acagcactgg
    14941 ttcagcgcct tgttccttct gtgttcaagg agccgaaaaa cattctgtac actctggatg
    15001 cgcgtgaaat gcgtgaccct gaacataaaa ctgaagctgg tcgcgccgca ggtatgcgcc
    15061 ttgctgcaca gattgactct gacctgattt ccatggttac gcagcgtgct actaacgtga
    15121 tcacaatgtc tgactcaacc acaggtacac agggccgtga tttgtggaac tgtgcggcag
    15181 gtattgatgc caccatgacg gcgattggtg tacctcaggg tatcaaccgt cgctctttct
    15241 ggaacccctt caactacaaa gaccttgctg gcgagcttgg tcaccgtgcc tacgctcagg
    15301 gcgcaaccct gacagcatac gaaaaagcgc agatccctcc ggttgcttcc tttgatagct
    15361 acaagaccga tatttctggt cgattaccga aaggaagcgc tgaatccttg acagtatcag
    15421 gccaacctga acacaaggtt gaagcgaaag attcaaatgg tatgccagtt gataaccgac
    15481 aggggactat tacggtatct gcatctggct tgcaggttgg tgatgcgttc accattgccg
    15541 gtgtgaattc cgtacaccag atcacaaaag ataccaccgg gcaaccgcag gtattccgtg
    15601 ttctggctgt tagcggaact accgtaacaa tctctccaaa gattctccct gttgaaaatg
    15661 ccgatgttgc gagtcgtcca tatgcaaacg tcgatgccaa accggcagaa tcagcagcaa
    15721 tcaccattct caacaagaac gcagcaccgg ctaacctatt ctgggctgat ggttctgttg
    15781 aactgatgta cggcaaactg gcattcccga ctggtcaggg gccacaggta atgacagcaa
    15841 ccaccgagca gggcgctacg ctgatcatgt cttacgcctt cgaccacatc aaaggcgtaa
    15901 ccactgcacg tttcaccact ctgtacggtt gctctgtact ggttcctgaa tatacgggca
    15961 tcgttattgc cgggcagtaa ttttggtggg gcttcggccc catttttatt gggagaagac
    16021 aatggcacga acaatgctct ataagccggg caacatgatc acctgtggtc agtttgctgt
    16081 cgattacatc attgttgatg acgaagaagt taaatctcac ctgaaaaaag gttgggtaaa
    16141 aactcctgaa gaaaccgcaa cgaagcaaaa agtggctaag gcggaagaag atggcgaaaa
    16201 cgaagggtga tctcgttcta aaggctttac gaaaagccgg gctgtattcc aatgccacgt
    16261 tgacagatgc tgaccctcag gcaattgaag atgccattaa tgacctcgaa gacatgatgg
    16321 cagcatggca ggcgaaaggt atcgagcttg ggtatcagtt tgctgataca gaaaacggca
    16381 tcatgccgtt acctgacgat gattcaggta tccccgcatg ggcaaatgat ggtgtcgctt
    16441 tgaaactcgc tgtgcaagtg tgcatggata acgtcattca gccgtcagac gctctcctta
    16501 ccgctgctga cagtgcatat cagacaatct gcatcgcttt aaccaaaata ccaccacttg
    16561 agcggcgaaa tgacatgcct cgcggtagtg gcaacaaaag cgcgtttacg tggaatcggt
    16621 tttacatcga gaaagatgat cagagtacgt gaggtgaata aatgccgatt cagcaacttc
    16681 cgcttatgaa aggtgtcggc aaagatttcc gaaacgccga ctatatcgac tatctgccag
    16741 tgaatatgtt ggctacaccc aaagaaatcc tcaacagcag cggatatctt cgctcattcc
    16801 cgggcattgc caaacgttct gatgtgaacg gtgtatctcg aggcgtcgag tacaacatgg
    16861 cgcagaatgc tgtctatcgt gtgtgtggcg gcaagctcta caaaggagaa agtgaagtcg
    16921 gtgatgttgc cggaagtggt cgcgtatcaa tggcgcatgg tcgtacatct caggcggtag
    16981 gcgttaatgg tcaactggtc gagtatcgct atgatggcac ggttaaaacc gtctcaaact
    17041 ggcctacaga cagcggattc acgcagtatg agttaggctc agtccgcgac attacgcgct
    17101 tacgtgggcg ttatgcgtgg tcaaaagacg gcactgattc atggtttatc actgaccttg
    17161 aagacgaatc gcatcctgac cgctacagcg cacaatatcg cgcagaatct cagccggacg
    17221 gcatcatcgg tatcggaaca tggcgagact tcatcgtctg ctttggttca tcgacgattg
    17281 aatatttctc cctgactggc gcaaccaccg ttggtgctgc tttgtatgtc gcccagccat
    17341 cattgatggt gcaaaaaggc atcgccggga cttactgcaa aacgccgttt gctgattcct
    17401 atgctttcat cagcaatccg gcaacaggtg cgccgtctgt atacatcatc ggctccggtc
    17461 aggtgtcacc aatcgccagc gcgagcattg agaaaatcct ccgctcctac actgctgatg
    17521 aactggctga tggtgtgatg gaatcgttgc ggtttgatgc tcatgagttg ctgattatcc
    17581 atcttccgcg ccatgttctg gtgtacgacg catcttcaag cgccaatggt ccgcaatggt
    17641 gtgtgctgaa gacaggcctg tatgacgatg tgtaccgcgc tatcgacttc atttacgaag
    17701 gcaatcagat aacgtgcggc gataagctgg aatctgtcac cgggaaactg caattcgata
    17761 tcagcagcca gtatgacaag cagcaggaac acctgctgtt tactccactc ttcaaagcag
    17821 ataacgccag atgctttgat ctggaggtgg aatcatcgac gggtgttgct cagtacgctg
    17881 accgcctgtt cctatcggca accactgacg gcatcaatta cggacgtgag cagatgattg
    17941 agcagaatga accgttcgtt tacgacaagc gcgttttgtg gaagcgtgtc gggcgcatca
    18001 ggaaaaatgt cggcttcaga ttgcgcgtta tcacgaagtc acctgtcact ctgtcaggct
    18061 gccagataag gattgagtaa tggctgattc gaatctcaac acccctgtta ttatgcaggc
    18121 gacgcggctc gatacatcaa tccttccacg caatatattc agccagtctt acctgcttta
    18181 cgttatcgca cagggtactg atgttggtaa cgtggcgaac aaggccaacg aggccggaca
    18241 gggcgcttat gacgcacaag tcaggaacga tgagcaggat gtgattctcg ctgaccatga
    18301 gcagcgaatt tctgcagcgg aagcaacgct tgttaatcat gaggagcgaa tcagtcaggc
    18361 agaatcaact cttcaggacc atgaaacacg aatagctcag aatgaaagcg atattgcgtc
    18421 gcttgatacc agagttcagt cgctggagtc gcaggtttca gaccatgaaa cgcgcatcga
    18481 tgctctggag tatgccacta ctcgcaagaa gtcagaggtt gtttactctg gcgtatctgt
    18541 aaccatcccg acagcgccga ccaaccttgt tagcctgctg aaaacgctca cgccgtcatc
    18601 cggcacgttg gcaccattct tcgacaccgt taacaacaag atggttgtgt tcaacgagaa
    18661 caaaaccttg ttcttcaagc tgtcgattgt cgggacgtgg cccagcggaa ccgccaacag
    18721 gtcaatgcag ctaacctttt ccggctctgt ccctgacacg ctggtaagca gtcgtaatgc
    18781 ggcgacaacg accgataaca tcctgttagc tacgttcttc agcgtggata aagacggctt
    18841 tcttgccaca aatggcagca cgttaaccat tcagtctaat ggtgcggcat ttactgccac
    18901 aaccatcaaa atcattgcgg agcagtgatg gaaataaagc tcatcgataa tccggtgaag
    18961 cttgcagaat tcctcaacaa cccggcaaac acggggaata tcgtagacag tggagacaaa
    19021 tactacatca agcctgatgc ggtatacctc ggcatctacg aaggactgat gctggtcgga
    19081 gtgcatgaag tgcgtaactt ctggcatagc gttgttgaat gccatgcggt gtacgacccc
    19141 ggattccgtg gcgaatatgc actgcaaggg catcgattat tctgcaaatg gcttctcgaa
    19201 aactcaccat tccttaacag cattaccatg gttcctgaca ccacgaaata cggacggtca
    19261 attatccgtt tgcttggcgc tacccgtgtt ggtcaccttg atgatgctta taccagcaat
    19321 ggaaagcctg taggcatcac gatttatcag ttaccgcgct caaaatacga ggagctaaag
    19381 aatgttaatt ttccagattg ccaataagca cctcagcaaa gctgtttact gcaaaggcgg
    19441 cagtgatggc ggttcaaaag cccaggcacg cgcaactgaa aagggtatcg aattgcagcg
    19501 tgaaatgtgg caaacgaaca tgcaaaacct cgcaccgttc acgccactcg ctcaacagta
    19561 cgtatcagag ttgcagaatc tttcctctct tcaggggcaa ggtcaggcgc ttaaccagta
    19621 ttacaactct cagcagtata aagaccttgc aggtcaggcg cgttaccaga gtctggcagc
    19681 agcagaggca acgggtggat taggctctac agcaacagga aaccagttag cagcaatcgc
    19741 acctacactc ggtcaaaact ggctgtcagg tcagatgaac aactacaaca atctggcaaa
    19801 tatcggcctt ggtgctctta caggtcaggc aaatgccgga cagaactacg ctaacaacgt
    19861 cagccaactg tatcaacagc aggcggcagc atctgcggcg aatgctaacc gaccatcagg
    19921 actgcaatca gccttgggag gtgccatgag cggtgcggca tcaggggcga tgattggctc
    19981 tgtggtgcca ggaataggta cggctgttgg cgctattggt ggcggcatta tcggtggtct
    20041 tggatcattg ttttaaggtg ggaatatggc tacttggcaa caaggaatca actcaggcgg
    20101 ctttcttgct ggtatcggtg ggcaaaactc aaatgcgcca aaggcaagtg atgtaagtga
    20161 ggcgttggcc tatattcgcc agaacaacga aatggagcgc tcaggtcgca ataacatcgg
    20221 ccttcaggcg ttgcaggggc ttggtagtgt cgctcaaaca tatcaagccg caaagcaaca
    20281 ggaagtggat gctgcattcc aaaaagaata tgcggcagcc atccagtcag gtgatcgaca
    20341 gcaggtgcga gatctgatga ccaaatatcc tggtcaatta gagaagattc agtctggtat
    20401 gaagtgggca gacgaagacc agcgcaattc tatcggcacc ttagcggctg gcgcacgcct
    20461 tgcagcctcg tctccagaag caatgcaatc atggctgcaa aacaacgcca aggaactgac
    20521 tcgcgtcggt gttgacccta acaacgttgc tcagatgtat cagcagaacc cttcaggatt
    20581 tggtgagttt gttgatcatc ttggaatggc cgcgcttggt ccgattgatt acttcaatgt
    20641 tcaggacaag atggcgggtc gtgagattga ccgaggcaga ctggcagaga caatccgcag
    20701 caatcaggct ggtgaagcac ttcaggcgag agggcaggat attagccgag caaatgcgtt
    20761 aacgtcagca tatgcaccaa cagccgcaat gcagaattac aatcagtacg cgcaaatgtt
    20821 aaaggtagat ccagatggtg cagcggcatt tgcggcagcg gcgggaatta atcccaatgc
    20881 taagaaatta cttaaggttg aaaccaatcc tgatggctcg gtaactaagt attacaccga
    20941 tggcagcgag gaagccggaa aactaaacca acctatatct ggtgatggca ttaaaccaat
    21001 tagcttgcca caagcgcaaa gcatcataga taaggctaat gagggttcca agaaggcggc
    21061 gggatttgct ttgcgattaa aagattcaat ggactcaatg aatcagctta gtaaaagcat
    21121 tgaccctaag cgagttgcat taataaatcg ctctcttggt gatgggacta ttgcaaattt
    21181 aagcctatca ccagcggagc agcaatatat ggtaaatgcg agagacgcct tgtatgcaat
    21241 tttgcgccca gaaacaggtg cagcaattac tctgccagag atgcaggagt attccaaaat
    21301 gtacctgcct cagcccggtg attccaaggc tgctactgaa acaaaaatgc gaaaaatgca
    21361 gggccaatat aactcattac gtggtcagtc tggtcgcgtt tatgatgctt tggtggtttc
    21421 aagtgctgca aatagtcaac aacagagcaa tagccaacaa ccgacaaata cccaacagca
    21481 gcagagtcaa tccggatcat atacctcaaa atcaggcatt caatttacgg tggaatgatg
    21541 aaagtaactg caaacggtaa gacatttacc tttcctgatg gtacgagcac cgaagatatt
    21601 ggcaccgcca ttgatgagta ttttgctggt caggctgttc agcaacaaac agttaatcag
    21661 gccaataatg caccaacacg ggaagaacca tcattgatgc aacaagctgg cgattggctc
    21721 actggtggtc aaagtgcagg gcaaattgca gaacaggctg gtcgtggtct ggtaaacata
    21781 ccatttgacg tattgcaggg tggcgcaagt ctgattaatg caatcagtca ggggcttggt
    21841 gggccaaaag ttttggatga tgtttatcgt ccagtagaca gaccgacaga cccctacgct
    21901 caagctggag aaacaattgg cgggtattta gttccaggaa ttggaacggc aggaagcatg
    21961 gctattggat cactggcaga ggccgcaaat cagaaaggcg atttcgcaca aaatgcagct
    22021 aaaaatgccg gagttaacct tgccgctcag ggtgttcttt ccgcagcagc aaagggaata
    22081 gggcgtggaa taacggctat aaaaggtgat attgcgccag aagtggcgaa gaaaattgcc
    22141 acttcagaat cgatgggcgt gacaccaatg acatctgatg ttatcccgcc gaaaaatgct
    22201 ttcactcgcg gccttactca ggatgccgag ggggctttgc tcgggacagg ctcaaagcga
    22261 gcggagcaat atgcaacgcg tagtaagctg gtaagcaatt attttgaccg ttttggtgag
    22321 tacaaccctg atgatgtggt gaaatctctg accaccacgt taagggggcg gaaggatgct
    22381 gctggcgctg ttatcaatga cgtcaccaat aaaatgggta atgccgcagt tgataccaca
    22441 aataccatga atgctctgaa tacagcgatc gcaagacagg aacggcttgg gacttcagcc
    22501 aatcaaagcc tgcttacatc cttgcgtaac ctacgtgaag aattagcaaa ccctgcaact
    22561 gatttggatg ttacgtttga tctcttgcgt cagcacagaa cagcatttag atctaatgtt
    22621 cagggagatt ctatggtctt ccccaaccag gcaaaagcag ctaccaatat ggtagagaat
    22681 gcaatgtcaa aagaccttcg taacgcagtt gctaaaaacc tcggtgcatc agacgcagca
    22741 aaatacctta aagcaaattc agattatgca aacgtttata ataaggtgct taataaaaac
    22801 attgctaaca agctcaacaa ggcaagcagt gaagccagtc ctgaacttat aaataccgtt
    22861 gtattaagca gaaaaccatc tgacgtgaaa cgaatctgga gcgcattgga tgataaaggg
    22921 aaagatgcta tgcgtgcagc ttacgtcagc aaaatagcgg aaaaggccgg tgattctcca
    22981 gccaagttca tcactgaagt taataagctg aaatctcagt caggtggtga aatttacaac
    23041 actatttttt ctggaaagca catgaaagag cttgattctc ttcatgaagt tctacagcaa
    23101 acagcaaggt cagacaccgc aaatgtagta actcagacgg ggcaatcgca agccaaccgg
    23161 ataaggacga ttggcgcaac tgcgactctt ggcgtatcaa tggggcttga ggctggcttt
    23221 ggtgcaatga tgcgtttgta tgagtctaaa gcagcaagga atgctctctt acgtttggca
    23281 aacaccaaag caggaacacc agcctatgaa agagcgctga ataacgccgc aaatgccatc
    23341 agaccaatac ttgccaatga tgcaactgat cgctaagggt tgatgtcatg gatgttgtta
    23401 attgcgaatg gaatcgcgga actctttgct tgatagaaaa ggatctgttc tccttaactc
    23461 atcatagttt ttattaaact ttcttgcttc acctgggtat ctgttgaaca aaacattgca
    23521 gaaaaaagag agcaataact cacatatgaa tgctaatacc ctgaaaaata aaggtataaa
    23581 aagaagggaa ggttcattga agtctctatt ccaaacaaaa tagacaatca tcccgacata
    23641 ccaaattgat atccctatac tgatgattgt tccaacagag gtaactacga tcccgattaa
    23701 gtcggacagt ctgtttgcca gtaactcatt tgagtatctt gcaatcatat accgccaaca
    23761 ttttgcaata tcatcctgat ctgtagagtc ttctggaata gctaggcttc tacatattga
    23821 agtagctctt tttttctcaa ctctttttga gagccatcca ttaaagagat acatcaacac
    23881 ctgcgttgct attcctgcac caagtgatat caaagacatc cccaaaaatc gccatcctgg
    23941 agatagtgac tggtactgcc aaacaaaaaa aattaaaaaa cccaatgagc ctatgacata
    24001 ggctg
//
LOCUS       DAZFAH010000053        23986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFAH010000053 DAZFAH010000000
VERSION     DAZFAH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.053"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..2913
                     /locus_tag="ACHJWB_002026"
                     /pseudo
     CDS             19..2913
                     /locus_tag="ACHJWB_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2952..3809)
                     /gene="alkA"
                     /locus_tag="ACHJWB_002027"
     CDS             complement(2952..3809)
                     /gene="alkA"
                     /locus_tag="ACHJWB_002027"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4313667.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            3943..5295
                     /gene="yegD"
                     /locus_tag="ACHJWB_002028"
     CDS             3943..5295
                     /gene="yegD"
                     /locus_tag="ACHJWB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4313668.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5307..7247)
                     /gene="yegI"
                     /locus_tag="ACHJWB_002029"
     CDS             complement(5307..7247)
                     /gene="yegI"
                     /locus_tag="ACHJWB_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4313669.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(7244..8005)
                     /gene="pphC"
                     /locus_tag="ACHJWB_002030"
     CDS             complement(7244..8005)
                     /gene="pphC"
                     /locus_tag="ACHJWB_002030"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4313670.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8002..8661)
                     /gene="yegL"
                     /locus_tag="ACHJWB_002031"
     CDS             complement(8002..8661)
                     /gene="yegL"
                     /locus_tag="ACHJWB_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4313671.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(8882..8941)
                     /gene="ibsA"
                     /locus_tag="ACHJWB_002032"
     CDS             complement(8882..8941)
                     /gene="ibsA"
                     /locus_tag="ACHJWB_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4313672.1"
                     /translation="MMKLVIILVILLVISFQAY"
     gene            complement(9214..9270)
                     /locus_tag="ACHJWB_002033"
     CDS             complement(9214..9270)
                     /locus_tag="ACHJWB_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4313673.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            9549..10790
                     /gene="mdtA"
                     /locus_tag="ACHJWB_002034"
     CDS             9549..10790
                     /gene="mdtA"
                     /locus_tag="ACHJWB_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4313674.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            10790..13912
                     /gene="mdtB"
                     /locus_tag="ACHJWB_002035"
     CDS             10790..13912
                     /gene="mdtB"
                     /locus_tag="ACHJWB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4313675.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFTLAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            13913..16990
                     /gene="mdtC"
                     /locus_tag="ACHJWB_002036"
     CDS             13913..16990
                     /gene="mdtC"
                     /locus_tag="ACHJWB_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4313676.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSRMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIWAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            16991..18406
                     /gene="mdtD"
                     /locus_tag="ACHJWB_002037"
     CDS             16991..18406
                     /gene="mdtD"
                     /locus_tag="ACHJWB_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313677.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            18403..19806
                     /gene="baeS"
                     /locus_tag="ACHJWB_002038"
     CDS             18403..19806
                     /gene="baeS"
                     /locus_tag="ACHJWB_002038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4313678.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLHNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            19803..20525
                     /gene="baeR"
                     /locus_tag="ACHJWB_002039"
     CDS             19803..20525
                     /gene="baeR"
                     /locus_tag="ACHJWB_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4313679.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPRKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            20716..21048
                     /gene="yegP"
                     /locus_tag="ACHJWB_002040"
     CDS             20716..21048
                     /gene="yegP"
                     /locus_tag="ACHJWB_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4313680.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            21257..21553
                     /locus_tag="ACHJWB_002041"
     CDS             21257..21553
                     /locus_tag="ACHJWB_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4313681.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            21555..21851
                     /locus_tag="ACHJWB_002042"
     CDS             21555..21851
                     /locus_tag="ACHJWB_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4313682.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            21954..23315
                     /gene="yegQ"
                     /locus_tag="ACHJWB_002043"
     CDS             21954..23315
                     /gene="yegQ"
                     /locus_tag="ACHJWB_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4313683.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     VEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23646..23963)
                     /gene="yegR"
                     /locus_tag="ACHJWB_002044"
     CDS             complement(23646..23963)
                     /gene="yegR"
                     /locus_tag="ACHJWB_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO4313684.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         5588 a         6397 c         6366 g         5635 t
ORIGIN      
        1 cgaataaagt aacggggtct ggttgtcctg ctgacgcccg gagacgatcc tctcagggca
       61 tttttgatat gggtactgtc agaatccatc ggcgctctgg cactggtgcc gctgggattg
      121 ttatttaaac cacactatct gctgcgccat cgcaacccac ggttgctttt tgagtcgctg
      181 ctcacgttag ccatcacact gacgttaagc tggctttcga tgctgtatct gccgtggcct
      241 tttactttca ttattgtgct gttgatgtgg agcgccgtgc gtctgccacg aatggaagcc
      301 tttttgatct tccttaccac ggtgatgatg gtgtcgctga tgatggccgc ggatccctcc
      361 ctgcttgcta cgccgcgtac gtacctgatg agccatatgc cgtggctacc gtttttgctg
      421 atcctgctgc ccgccaacat catgaccatg gtgatgtacg catttcgtac tgaacgaaag
      481 catatttccg aaagcgaaac ccgttttcgc aacgcgatgg agtattccgc catcggcatg
      541 gcccttgtgg gaacggaagg acaatggctg caagccaata aagccttatg tcagtttctc
      601 ggttacagtc aggaagagct gcgcggactc acctttcagc aactgacctg gccggaggat
      661 ctcaataaag atctccaaca ggttgaaaag ctgataagcg gtgaaataaa cacctattca
      721 atggaaaaac gctactacaa ccgtaatggc gatgttgtct gggcgttgct tgccgtctca
      781 ctggtgcgcc acacggatgg cacgccgctc tattttatcg ctcagattga agacattaac
      841 gagctaaaac gcaccgaaca ggtgaatcag caactgatgg aacgcatcac gctggctaac
      901 gaagcgggcg ggattggcat ctgggagtgg gagttgaagc cgaatatttt tagctgggat
      961 aagcggatgt tcgagctgta tgaaattcct ccacatatca aaccgaactg gcaggtgtgg
     1021 tacgagtgcg tgctgccgga agatcgccag catgccgaaa aagtgattcg tgattcgttg
     1081 caatcacgct cgccctttaa actggaattt cgcattaccg taaaagacgg tattcgccat
     1141 atccgcgccc tcgccaaccg ggtactgaat aaagaaggcg aagtcgaacg cctgctcggc
     1201 attaatatgg atatgactga ggttaaacag cttaacgagg cattgtttca ggaaaaagag
     1261 cgcctgcaca ttacgctgga ctccatcggc gaagccgtgg tctgtattga tatggcaatg
     1321 aaaattacct ttatgaatcc agtggcggag aagatgagcg gctggacgca ggaagaagca
     1381 ttaggtgttc cgctcctgac ggtgttgcat attacctttg gcgacaatgg tcccctgata
     1441 gagaacattt acagtgccga cacctcacgt tccgcgattg aacaagatgt ggtgttgcac
     1501 tgtcggagcg gcggcagcta cgacgtgcat tacagtatta cgccgttaag tactctggac
     1561 ggcagcaata ttggttcggt tctggtgatt caggacgtta ccgaatcacg caaaatgctg
     1621 cgccagctga gctacagcgc ctcccatgat gcactgacgc atctcgccaa ccgcgccagt
     1681 tttgagaaac aactgcgtat cctgctgcaa acggtaaaca gtacacatca gcgacatgcc
     1741 ctggtgttta tcgatcttga tcgctttaaa gcggtgaatg acagcgccgg gcatgcggcg
     1801 ggtgacgctt tactgcgcga actggcgtcg ttgatgctga gtatgctgcg ctccagcgac
     1861 gtgctggcgc gactcggtgg tgatgaattt ggtctactgt tgccagactg taatatcgaa
     1921 agtgcgcgtt ttatcgctac acgcattatc agtgccgtga atgattatca ctttatatgg
     1981 gaaggccgtg tgcatcgggt aggtgccagt gccgggatta ccttgattga tgacaacaat
     2041 catcaggcgg cagaagtgat gtcgcaggct gatatcgcct gttatgcctc caaaaatggt
     2101 ggacggggcc gggtgacggt ttacgaacca cagcaagctg ccgcacatag cgaacgggcg
     2161 gcgatgtcgc ttgatgaaca gtggcggatg attaaagaga atcagttgat gatgatggcc
     2221 cacggtgtcg cttcgccgcg gatcccggaa gcgcgtaatt tgtggctgat ttcgcttaaa
     2281 ctctggagtt gcgaaggcga gattattgat gaacaaaaat ttcgtcgtag cttcagcgat
     2341 ccggcactta gccatgctct tgaccgacgg gtattccacg aatttttcca gctggccgca
     2401 aaagcggttg ccagtaaagg cataagcatc gccctccccc tttccgttgc cggtttgagt
     2461 agcgccacgc tggtgaatga tctgcttgag cagctggaaa atagccctct accaccacgg
     2521 ttattacatc tgattattcc ggcagacgcg attttagatc acgcagaaag cgtgcaaaaa
     2581 ctgcggctgg cgggatgtcg gatagtgctc agccaggtgg gccgcgatct gcaaatcttc
     2641 aactcgctga aagcgaatat ggcagattac ctgctacttg atggtgagtt atgcgccaac
     2701 gtgcagggta atttgatgga tgagatgctg attacgatta ttcaggggca cgctcagcga
     2761 ctcgggatga aaaccatcgc cgggccagtc gttttaccct tagtgatgga tacgctttct
     2821 ggcatcggcg tcgatctgat ttatggtgat gtgattgccg atgcccaacc gctggatttg
     2881 ctggtgaata gcagttattt cgcgattaac tgagggagcg tttgcccatt gcctgatgcg
     2941 acgtatcagg cctacaattc gtccgttccg tctggttgcc agccttccgt ataccagata
     3001 tgcaacagcg cataagaacg ccagggcttc cagcgctcgg cataacggcg gatttgtgcc
     3061 ggtgtcatcc ccggaaatcg ctgtttaatc agataatcat ccggcagaaa aacatctttc
     3121 gcctgccagc cacgcaaagc gaaataattc gccgtccagc gcccgatacc cggaaaagtt
     3181 tgcagcgttt tcatcgcctg ctccacatcg cccggtattg tcattggtaa ggtgccctcc
     3241 agcgccgcat ttgccagatg aatcagcgcc tctgcccgtt tcaacggcat acctaacgct
     3301 tttaatgcct gcgggtcggc tgctgccagc cgctgaggcg tcgggaagca gatatactcc
     3361 ggaaaatcat ccagccgttc gccataaagc tgtaccactt tggcagtcaa ttttgccgcc
     3421 atcgccacgc tcaccagttg gcctaaaatc gcccgcacac cctgctcaaa agcatcaaca
     3481 cagccgggta aacgcaatcc gggccgggcc gcgcctaact tgcccaacgc accgttaaca
     3541 atctgcgggt tacattgcag atcaagcagg cggctcattt tcgccagaca ctcagcggca
     3601 acaggttcta aacctgcact taaatttatg tgcagagtat gacgggccat atccggaata
     3661 gcagtcacca cgccgcgata ttcgcccacc gccagactac gggcataata atcgtcagcg
     3721 accgtttcca caccactcac tgcacgggcg gcgagaaatc ccaacatcca cgaccagtca
     3781 tacggcggct gccagttcag ggtatacatc gcatctcctt attcatccgc tttcagcata
     3841 aacgttattc agacgctacg ctttgctttc atattccggt tgtcgcgacg gcaacatttc
     3901 gctaaagtca cgccccttct tcaccggcat ggggattaat tcgtgtttat tggttttgat
     3961 tacggtacag caaactgttc agtggcggtc atgcgtgacg gtaaaccgca attactgaaa
     4021 atggaaaacg acagcacgct gctgccttca atgctttgcg cgccaacgcg tgaagcagta
     4081 agtgaatggc tgtaccgcca tcatgatgtt ccggcagacg acgatgaaac gcaggcgctg
     4141 ttacgtcggg cgattcgtta taaccgcgaa gaagatatcg atgttacggc gaaaagcgtg
     4201 cagttcggtc tttcctcact ggcacagtac attgatgatc cagaagaagt gtggtttgtg
     4261 aaatcaccaa aatcgttcct cggtgccagc ggcttaaaac cgcagcaggt agcgctgttt
     4321 gaggatctgg tctgcgcaat gatgttgcac attcgccagc aggcgcaggc gcagctgcca
     4381 gaagcgatta ctcaggcggt gattggtcgc ccgatcaact tccaggggct gggcggcgat
     4441 gaagcaaacg cccaggcgca agggattctg gaacgcgcgg cgaagcgtgc cggattcagg
     4501 gacgtggtat tccagtacga gccggtcgcg gctgggctgg attacgaagc caccttgcag
     4561 gaagaaaaac gggtgctggt ggtggatatc ggcggtggta cgactgactg ttcattgctg
     4621 ctgatggggc cgcagtggcg ttcgcgtctc gatcgtgaag ccagcctgct gggtcacagt
     4681 ggttgccgta ttggcggtaa cgatctggac atcgcactgg cgtttaaaaa cctgatgccg
     4741 ttactgggca tgggtggcga aaccgaaaaa ggcatcgccc tgccgattct gccgtggtgg
     4801 aatgcggttg ccatcaacga cgtacctgcg cagagtgatt tctacagtag tgccaacggt
     4861 cgtctgctta acgatctggt acgcgatgcc cgcgaaccgg aaaaagtggc cctgctacag
     4921 aaagtctggc gtcagcgttt aagctatcgc ctggtgcgta gcgcagaaga gtgcaaaatt
     4981 gctctttcaa gcgtagcgga aacccgcgcc tcactgccgt ttatcagcga tgaactggct
     5041 acgctgatta gccagcaagg gctggaaagc gccctcagcc agccactggc gcggattctg
     5101 gaacaggtgc aactggccct ggataacgcc caggaaaaac cggacgttat ctacctgacc
     5161 ggcggtagcg cccgttcgcc gctgattaaa aaagcgctgg cagaacagtt gccgggaatt
     5221 ccgattgcag gcggcgatga ctttggctcc gtcaccgccg ggctggcacg ctgggcggaa
     5281 gtggtgtttc gttaaaagaa aatggctcag cagcggctta aatctgcctg cgcctgagcc
     5341 agtttttctg ccgcctgacg taaaggttcc atcaacggca tggtccgtgc tggagcatga
     5401 aggcggaatc gctgcaactc tgtcgcgccg acagtcaacg tcgattccag ccgatgtcgt
     5461 ttggcggtca ttttcgccat taccgcttgt ctgtctgctg gcgttatcgc ttcattcggc
     5521 ctgaacacaa aacggcgttc acagctcgct ttccagtcaa taaccgcctg ggtcagatgg
     5581 tcgccaaacc ctttcacttg cttaacgcca cgacgggtaa catctgcggc tgtttcaata
     5641 ccaaaagacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaacatca
     5701 ataaaaaatc cctccagaaa cttctgcttc tgccgttccc ttgcagtgtc atgaagtgct
     5761 gccagagcgc gtttctcttc ttccggtaac ccgagaattt cgtccttcat tttttcgagc
     5821 atcgcccgtt tggcgataaa accttccagc ccgcccaact gttggatctg gctgactaaa
     5881 tgctcatagt ccattttcgc gcgattaaat gcctcacggc gttgctggat ttctgctttg
     5941 tacgctttgc ttgtcagact gccaataatc cagatagccg acagcacagg aaccaaaata
     6001 atataacgcg gttctgcctg aaggccgcaa agcaacagcg ataacgctga cagtgcgatc
     6061 tcaatcagaa tgatgtattc gcgccgtaac aggcctaaag gaagcggcct gccagtcggt
     6121 tggaaatgat cgggtaatgg caattgcaat gctggcggtg ctactgacgc catcaccatc
     6181 gcccagactt tcgccagcac aaaatcaccg ctggtggtaa tgacctcttc gccgagatca
     6241 acaaaataga taacgccttg attatccagc gcacaccacg ggcagtcagc caaatgaccg
     6301 ggataaacat gcattgccga tacggtacat ttctttaact gttggcgtag agaatccagt
     6361 gctgataccc acgctttagc cgtcggacgc ccggttgcca cgccactttc cgtaaacgcc
     6421 tgctgaaaca tggcttcaac atcactcggt aacatcgata gcggaatcga tcgtggcggc
     6481 ggttttaaac cacgtcgctg attatctgac gcgtaggcat aacggaaatg ggtaatatcc
     6541 gtctccagcg cattacccgc atcagagata agcggcacac cagaataagg atgccggcca
     6601 ccaaacaaga cgtgaaaaat cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt
     6661 tcaaagccga caaatgacga caaggtttgt agctctggcg gcgtaaaatg cgacacgccg
     6721 acttcgcata aatgcagtgt gccattggcg ttaatctgaa aggagtcgct atcaatcaac
     6781 accactttgc tgtcgcgacc taccataaaa ctgttctggt ttacgtcacc cacgacgtgc
     6841 ccgtgctcgt gaaccgtagc aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa
     6901 tcccacgcac aatgagggta acgttgacga cgatgtgccg ggctatagat catatgaatc
     6961 ggttctttac cagaaacttt tggcatcata aaaccgataa cttttccgcc tcgtccaccg
     7021 tgaagcgttg cctgcggcca ggcgacataa ttcaacaatt gcgcgtcagc tgtcgcagcc
     7081 ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg
     7141 ctatcgacaa actcattgat atcataaacc gcgccttcac cgcctttgcc cagttcacgg
     7201 cctggcgtaa cgcactcacc agtagcagta tataaagtgg gtttcatggt aaccacagcg
     7261 ccagtgccaa cgttttatca tcatccgtac gctcgtttac cgccgggctg gacaaaaact
     7321 gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc agtccattga
     7381 aaaacggggt aaagaaaggc acatgaggag aattatccag catatttaac gccagtcgct
     7441 ggataccatc cgtaaatgct gcaactttat gcacacgctc agtgctggtg aaagtttcca
     7501 gtctggaaac ggcatcttca tcggtaatga agtgcgtcat attggcatat tcacccacca
     7561 tcggcgtaag cggcaattgc agaccgtgac caaaatccac caccacgccg ccatcgccaa
     7621 tctgcataat caaggtgcca ttagccgatg aaatcaatcc cagaaacgtg caggcaaaat
     7681 cgcgaactgc taattctttg gcttccgcct cggcaaaaag tcgctgacga acggtcagca
     7741 caatatccgt ggcgaggata tcattaagcc ccaattcccc accctgcact ttttgcgaca
     7801 tataagccat cgcttcattg acggcgagcg tcgcgccttc tcctccctgc gaaacgctac
     7861 ctgcgccatc agcaaggaac atcaccagta atggctgctg gtcatttagc caggcaacct
     7921 gcatctggca ggcatcctgg caaggcaagt ccgcgctgat gtgcgatgtt ccaacagcgg
     7981 atgcataaac cagacgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac
     8041 ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag
     8101 ttcacggaat tgtaatcctt gcagaggcaa aggctgacga acgctgattt gtgccagagt
     8161 cttcatatcc gccccctgaa caccgatgga aaagaaagca aaccttttat cttcttcccc
     8221 ccgaaacact ttgttggctg cagcctgcca ctcatcggtt ggtgcaccat cggtaatcag
     8281 gaaaatccac gggcgataat acgaaatacc atttgcacga tactcacgtt tccgctcctc
     8341 taccatatcc agggctttgg taatggccgc ccccattggt gtatcgccct gggcaaacag
     8401 gatgggcgga aaaaaattag cggcgctggt aaagggttgt tccacatgca ccgggccgaa
     8461 cgtcacaatg ccgagttcca ctctttttaa agccagcgaa tcggcaagca gctcatcgcg
     8521 aaaggtaacc aatccggtgt taagttcatt aataggtctg ccactcatcg agccagaaac
     8581 atccagcagc agaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc
     8641 aaacgtgatt tgttcgctca taagaaatcc tttcttagag aattattgag gtccggtatc
     8701 ataccgactg caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccggcg
     8761 gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg acactaaggg
     8821 cggagtgaca taatttcagg agtgagggtt agggagaggt ttccccctcc ccctggtgtt
     8881 cttagtaagc ctggaagcta atcactaaga gtatcaccag tatgatgacg agcttcatca
     8941 taaccctttc cttattaaaa gccctcttct ccgggagagg ctttcccgtt tcagcgtccc
     9001 gctgaaatca tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaat
     9061 aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa agaatatttg ctggcaggat
     9121 cgcagactac aaagcctgcg gattgacaat cttatcgtga aggcatactt tcaggagtga
     9181 gggtagagcg gggtttcccc cgccctggta gtcttagtaa gcggggaagc ttatgactaa
     9241 gagcaccacg atgatgagta gcttcatcat gaccctttcc ttatttaagg ccccttcctc
     9301 gggaggggct ttcccgtttc agcatccggc tgaaatctgc ggcttacctc ctttcgccct
     9361 gtatgcagtc tatcgctaac gcgtagataa aatagtttcc tgtgttatta ctggatgcgt
     9421 gctcgcaaat gtgcccgtca ttcagacgat tccagacagt gtttcataat tcctccattt
     9481 ttctccctta ttggctggct acactagtat cattccgcga aacgtttcag gaagagaaac
     9541 tcttaacgat gaaaggtagt tataaatccc gttgggtaat cgtggtggtt atcgccgcca
     9601 tcgccgcatt ctggttctgg caaggccgca atgactcccg gagtgcagcc ccaggggcga
     9661 cgaaacaagc gcagcaatcg ccagcgggtg gtcgacgtgg tatgcgttcc ggcccattag
     9721 ccccggttca ggcggcgacc gccgtagaac aggcagttcc gcgttacctc accgggcttg
     9781 gcaccattac cgccgctaat accgttacgg tgcgcagccg cgtggacggc caactgatag
     9841 cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca gaaattgacc
     9901 ccagccagtt caaagttgca ttagcacaag cccagggcca actggcaaaa gataaagcca
     9961 cgcttgccaa cgcccgccgt gacctggcgc gttatcaaca actggcaaaa accaatctcg
    10021 tttcccgcca ggaactggat gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta
    10081 aggctgatga agcaagcgtt gccagcgcgc agctgcaact cgactggagc cggattaccg
    10141 caccagtcga tggtcgcgtt ggtctcaagc aggttgatgt tggtaaccaa atctccagtg
    10201 gtgataccac cgggatcgtg gtgatcaccc agacgcatcc tatcgattta gtctttaccc
    10261 tgccggaaag cgatatcgct accgtagtgc aggcgcagaa agccggaaaa ccgctggtgg
    10321 tagaagcctg ggatcgcacc aactcgaaga aattaagtga aggcacgctg ttaagtctag
    10381 ataaccaaat cgatgccact accggtacga ttaaagtgaa agcacgcttt aataatcagg
    10441 atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac accgaacaaa
    10501 acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat tttgtctggg
    10561 tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt caggacagtc
    10621 agaaagtggt gatccgtgca ggtatttctg cgggcgatcg cgtggtgaca gacggcattg
    10681 atcgcctgac cgaaggggcg aaagtggaag tggtggaagc ccagagcgcc accactccag
    10741 aagagaaagc caccagccgc gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt
    10801 acccccgagc agcacaggcg gcccgtcgcg cctgtttatt atgcgtcctg tcgccaccac
    10861 gctgctgatg gtggcgatct tactcgccgg gattatcggt tatcgcgccc tgcccgtttc
    10921 ggcgctgccg gaagtggact atccgaccat tcaggtggtc acgctctacc caggtgccag
    10981 cccggatgtc atgacctctg ccgttaccgc gccgctcgaa cgccagttcg ggcagatgtc
    11041 tggcctgaaa cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt
    11101 ccagctaaca ttaccgctcg atgtcgccga gcaggaagtg caggccgcga ttaacgctgc
    11161 gaccaacttg ttgccgagcg atctgcctaa cccgccggtt tacagcaaag tgaacccggc
    11221 agatccgccg atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga
    11281 agatatggtg gaaacccgcg tcgcgcagaa aatctcgcag atttccggcg tcggcctggt
    11341 gacgctttcc ggcggtcagc gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc
    11401 cgccctcggc ctgaccagcg aaaccgtgcg caccgccatt accggcgcta acgttaactc
    11461 ggcaaaaggt agcctcgacg gcccttcccg tgcggtcacg ctttccgcga acgaccagat
    11521 gcaatccgcc gaagagtatc gccagctaat catcgcctac cagaacggcg cgccaattcg
    11581 tctgggcgat gtcgcaactg tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc
    11641 gaacaaagaa caggccattg tgatgaatgt tcagcgccag cccggtgcta acattatctc
    11701 caccgccgac agcattcggc agatgctgcc acagctcact gagagtctgc cgaaatcggt
    11761 gaaggtgaca gtgctttccg atcgcaccac caatatccgc gcatccgtcg atgatactca
    11821 gtttgaattg atgatggcta tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa
    11881 tattccggcg accatcattc ccggtgttgc tgtaccgctg tcgttaatcg gcactttcgc
    11941 ggttatggtg tttctcgatt tttcaatcaa taacctgaca ctgatggcgt taactatcgc
    12001 caccggattc gtggtcgatg acgccatcgt ggtgatcgaa aacatttccc gctatatcga
    12061 aaaaggcgaa aaaccgttgg cggcggcgct caagggcgca ggtgaaatcg gctttaccat
    12121 tatctcgctg accttctcac tgattgcggt gttgatccca ctgctgttta tgggcgatat
    12181 cgtcgggcga ctgttccgcg aatttgctat tacgctggcg gtagcgattt tgatctcagc
    12241 ggtggtgtcg ctgaccctga caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt
    12301 gcgtaaacag aaccgcttct cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc
    12361 ctatggtcgt ggactggcga aggtgctgaa tcatccgtgg ctgaccttaa gcgtggcgct
    12421 cagcacgctg ctgcttagcg ttctcttatg ggtgttcatt ccgaaaggtt tcttcccggt
    12481 acaggacaac ggcattattc agggcacttt gcaggcaccg caatccagct cctttgccaa
    12541 tatggcccag cgacaacgcc aggtcgcgga cgtgattttg caggatccgg cagtgcaaag
    12601 cctgacctca tttgttggcg ttgatggcac taacccgtcg ctgaatagtg cacgtttaca
    12661 aatcaacctc aaaccgctgg atgaacgtga tgaccgagtg caaaaagtca ttgcccgtct
    12721 gcaaacggcg gtggataaag tgccgggcgt cgatctcttc ctgcaaccaa cgcaggatct
    12781 gactattgat actcaggtca gccgcaccca gtaccagttt accttgcagg ccacctcact
    12841 ggatgcgctc agtacctggg tgccacagtt gatggaaaaa ctccagcaac tgccacagct
    12901 ttctgatgtc tccagcgact ggcaggacaa agggctggtg gcgtatgtca atgttgatcg
    12961 cgacagcgcc agccgtctgg ggatcagcat ggcggatgtc gataacgccc tgtacaacgc
    13021 gtttggtcag cgactgattt ccactattta tactcaggct aaccagtatc gcgtagtgct
    13081 ggagcacaac accgaaatta ccccgggcct cgcggcgctg gataccattc gcctgaccag
    13141 tagcgacggc ggcgtggtgc cgttaagctc aattgccaaa gttgaacagc gttttgcgcc
    13201 gctttccatc aaccatctgg atcagttccc ggtaacgacc atctccttta acgtgccgga
    13261 taactattcg ctgggcgatg cggtgcaggc gattatggac accgaaaaga cgctgaatct
    13321 gccggtggat atcaccacgc aatttcaggg cagcaccctc gccttccagt cggcgctggg
    13381 cagcactgtc tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct
    13441 gtacgagagc tttattcacc cgatcaccat tctctcgacg ctacccaccg caggggtcgg
    13501 cgcgctgctg gcattaatga ttgccggtag cgaactggat gtgattgcga ttatcggcat
    13561 tattttgctg atcggtattg tgaagaagaa cgccatcatg atgatcgact tcacgctggc
    13621 tgctgagcgc gagcaaggca tgtcgccgcg cgatgcaatc taccaggctt gtctgttgcg
    13681 ttttcgtccg atcctgatga ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt
    13741 gagtaccggg gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg tcggcggtct
    13801 gattgtcagc caggtgctga cgctgttcac cacgccggtg atttatctgc tgttcgaccg
    13861 cctggcattg tggaccaaaa gtcgctttgc tcgccatgaa gaggaggcgt aagtgaagtt
    13921 ttttgccctc ttcatttacc gcccggtggc gacgatttta ctgtcggtcg ccattaccct
    13981 gtgcggcata ctgggcttcc gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc
    14041 ggtgattatg gtcagcgcct cgctgcccgg tgcgtcacca gaaacaatgg cgtcttccgt
    14101 tgccacgccg ctggagcgct cacttgggcg cattgccgga gtcagtgaaa tgacctccag
    14161 cagttcgctc ggcagcacgc gtattatttt gcagtttgat tttgaccggg atatcaacgg
    14221 cgcggcgcgc gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc ccagccggat
    14281 gcccagccgt ccgacctatc gcaaagctaa cccgtcggat gcgccaatta tgatcctcac
    14341 gctgacatcc gatacttatt cgcagggtga actgtacgat tttgcctcga cgcagctggc
    14401 tccgacgatt tcgcaaatcg acggtgtcgg tgatgtcgat gttggcggca gctcgctgcc
    14461 cgccgtacgc gtcgggctga atccgcaggc gctgtttaat cagggcgtct cgctggacga
    14521 cgtacgcacc gccatcagca atgccaacgt gcgtaaaccg cagggggcgc tggaagatgg
    14581 cactcaccgc tggcagatcc agaccaatga tgagctaaaa accgccgccg aatatcagcc
    14641 gttgattatt cattacaaca acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac
    14701 cgactcggtg caggatgtgc gcaacgccgg gatgaccaac gccaaaccgg ctattttgct
    14761 gatgatccgc aagctgccgg aagccaatat tatccagacg gtagacagca tctgggcaaa
    14821 attaccggag ttgcaggaaa ccattccggc ggcgattgat ctgcaaattg ctcaggatcg
    14881 ctcccccacc attcgcgcct cgctggaaga agtcgagcaa acgctgatta tctcggtggc
    14941 gctggtgatt ctggtggtgt ttttattcct gcgctcgggt cgcgccacta ttattcccgc
    15001 cgttgcggtg ccggtttcgc tgattggtac gtttgcggcg atgtacctgt gcggattcag
    15061 tctcaataac ctttcgttaa tggcgctcac catcgctact ggtttcgtgg tggatgacgc
    15121 catcgtggtg ctggaaaaca ttgcccgtca tctggaagcg gggatgaaac cgctacaggc
    15181 cgcgctacaa ggtactcgcg aagtcggctt tacggtgctg tcgatgagtc tgtcactggt
    15241 ggcggtgttc ctgccgctgc tgttgatggg cggattgccg ggccgactgt tacgcgaatt
    15301 tgccgtgacg ctttctgtcg ccattgttat atcgttgctg gtttctctga cattaacgcc
    15361 aatgatgtgt ggctggatgc tgaaagccag caagccgcgt gagcaaaagc gactgcgtgg
    15421 ttttggccgc atgttggtag ccctgcaaca aggctacggc aagtcgctga aatgggtgct
    15481 caatcatacc cgtctggtgg gtgtggtgct gcttggcacc attgcgctga atatctggct
    15541 gtatatctcg atcccgaaaa ccttcttccc ggagcaggac accggcgtgc tgatgggcgg
    15601 gatacaggcg gatcagagca tttcgtttca ggcgatgcgc ggcaaattgc aggatttcat
    15661 gaaaattatc cgtgacgatc cggcagtgga taatgtcacc ggctttaccg gcggttcacg
    15721 agttaacagt gggatgatgt ttatcaccct caagccacgc gacgaacgca gcgaaacggc
    15781 gcagcaaatt atcgaccgtc tgcgcgtaaa actggcgaaa gaaccggggg cgaatctgtt
    15841 cctgatggcg gtacaggata ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta
    15901 cacgttgtta tccgacgacc tggcggcact gcgcgaatgg gagccgaaaa tccgcaaaaa
    15961 actggcgacg ttgccggaac tggcggacgt gaactccgat cagcaggata acggcgcgga
    16021 gatgaatctg gtttacgacc gtgacaccat ggcacggctg ggaatcgacg tacaagccgc
    16081 caacagtctg ttaaataacg ccttcggtca gcggcaaatc tcgaccattt accagccgat
    16141 gaaccagtat aaagtggtga tggaagtgga tccgcgctat acccaggaca tcagtgcgct
    16201 ggaaaaaatg ttcgttatca ataacgaagg caaagcgatc ccgctgtcgt atttcgctaa
    16261 atggcaaccg gcgaatgccc cactatcagt gaatcatcag ggattatcgg cggcctcgac
    16321 catttcgttc aacctgccga ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg
    16381 cgcaatgacc cagcttggtg tgccttcgac ggtgcgcggc agttttgccg gaacagcgca
    16441 ggtgttccag gagacgatga actcacaggt gatcctgatt atcgccgcca tcgccacggt
    16501 gtatatcgtg ctgggtatcc tttacgagag ttatgtgcac ccgctgacga ttctctccac
    16561 cctgccctcg gcgggtgttg gagcgctgct ggcgctggag ctgttcaatg ccccgttcag
    16621 cctaatcgcc ctgataggga tcatgctatt aatcggcatc gtgaagaaaa acgccattat
    16681 gatggtcgat tttgcgcttg aagcccaacg gcacggtaac ctgacgccgc aggaagctat
    16741 tttccaggcc tgtctgctgc gttttcgccc gattatgatg actaccctgg cggcgctgtt
    16801 tggcgcgctg ccgctggtgt tgtcgggcgg cgacggctcg gagctgcggc aacccctggg
    16861 gatcaccatt gtcggcggac tggtaatgag ccagctcctt acgctgtata ccacgccggt
    16921 ggtgtatctc tttttcgacc gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt
    16981 aaccgagtaa atgacagatc ttcccgacag cacccgttgg caattgtgga ttgtggcttt
    17041 cggcttcttt atgcagtcgc tggacaccac catcgtaaac accgcccttc cctcaatggc
    17101 gcaaagcctc ggggaaagtc cgttgcatat gcacatggtc attgtctctt atgtgctgac
    17161 cgtggcggtg atgctgcccg ccagcggctg gctggcggac aaagtcggcg tgcgcaatat
    17221 tttctttacc gccatcgtgc tgtttactct cggttcactg ttttgcgcgc tttccggcac
    17281 gctgaacgaa ctgttgctgg cacgcgcgtt acagggcgtt ggcggcgcga tgatggtgcc
    17341 ggtcggcaga ttgacggtga tgaaaatcgt accgcgcgag caatatatgg cggcgatgac
    17401 ctttgtcacg ttacccggtc aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct
    17461 ggtggagtac gcgtcgtggc actggatctt tttgatcaac attccggtgg ggattatcgg
    17521 tgcgatcgcc acattgatgt taatgccgaa ctacaccatg cagacgcggc gctttgatct
    17581 ttccggcttt ttattgctgg cggttggcat ggcggtatta accctggcgc tggacggcag
    17641 taaaggtaca ggtttatcgc cgctggcgat tgctggcctg gtcgcagttg gcgtggtggc
    17701 actggtgctt tatctgctgc acgccagaaa taacaaccgc gccctgttca gtctgaaact
    17761 gttccgcact cgtacctttt cgctgggcct ggcggggagc tttgccggac gtattggcag
    17821 tggcatgttg ccctttatga caccggtttt cctgcaaatt ggcctcggtt tctcgccgtt
    17881 tcatgccgga ctgatgatga tcccgatggt gcttggcagc atgggaatga agcgaattgt
    17941 ggttcaggtg gtgaatcgct ttggttatcg tcgggtactg gtagcgacca cgctgggtct
    18001 gtcgctggtc accctgttgt ttatgactac cgctctgttg ggctggtact acgttttgcc
    18061 gttcgtcctg tttttacaag gaatggtcaa ctcaacgcgt ttctcctcca tgaacaccct
    18121 gacgctgaaa gatctcccgg acaatctggc gagcagcgga aacagcctgc tgtcgatgat
    18181 tatgcaattg tcgatgagta tcggcgtcac catcgccggg ctgttgctgg gactttttgg
    18241 ttcgcagcat gtcagcgtcg acagcggcac cacacaaacc gtctttatgt acacctggct
    18301 tagcatggcg ttgatcatcg cccttccggc gttcatcttt gccagagtgc cgaacgatac
    18361 gcatcaaaat gtagctattt cgcggcgaaa aaggagcgcg caatgaagtt ctggcgaccc
    18421 ggtattaccg gcaaactgtt tctggcgatt ttcgccacct gcattgtctt gctgatcagt
    18481 atgcactggg cggtgcgtat cagttttgag cgtggcttta ttgattacat caagcatggt
    18541 aatgaacaac gattacaact gttaagtgat gcgcttggcg agcagtatgc gcagcatggc
    18601 aactggcgct tcctgcacaa caatgatcgc tttgtctttc agatcctgcg ttcatttgaa
    18661 cacgataatt cggaagataa acccggcccg ggtatgccac cgcacggctg gcgtacccag
    18721 ttctgggtgg ttgatcaaaa caacaaagtg ctggttggtc cgcgagcgcc gattccacct
    18781 gacggtacac ggcgacccat tctggtcaac ggtgcggaag ttggcgcggt gatcgcctcc
    18841 cccgttgagc ggttaacgcg caatactgat atcaatttcg ataaacaaca gcggcaaacc
    18901 agctggctga tcgtcgccct ggcaacgtta ctcgcggcgc tcgccacctt tctgctggcg
    18961 cgcggtttgc tggcaccggt aaaacgactt gtcgatggca cgcacaaact ggcggcgggc
    19021 gatttcacta cccgcgtaac gcccaccagt gaagatgaac tgggcaaact ggcgcaagac
    19081 ttcaaccagc ttgccagcac gctggagaaa aaccagcaaa tgcggcgcga ttttatggcc
    19141 gatatttcac acgaactgcg tacgccatta gcggtgctgc gcggtgaact ggaagccatt
    19201 caagatggcg tgcgcaaatt cacgccggag acggtggcgt ctttacaggc ggaagtcggt
    19261 acactgacta aactggttga cgatcttcat cagttgtcga tgtctgatga aggcgctctc
    19321 gcctatcaaa aagcaccggt agatttgatc ccactgctgg aagtggcggg cggcgcattt
    19381 cgcgaacgat ttgccagccg tggcctgaaa ctgcagtttt ccctgccaga cagtattacc
    19441 gtatttggcg atcgcgaccg tttaatgcag ttattcaata acttactcga aaacagcctg
    19501 cgctacactg atagcggcgg cagcctgaaa atctctgccg agcagcacga caaaacggtg
    19561 cgcctgacct ttgccgacag tgcgccaggt gtcagtgacg agcagctaca aaaattattt
    19621 gaacgttttt atcgcaccga aggttcccgc aaccgcgcca gcggtggttc cgggctgggg
    19681 ctggcgattt gcctgaacat tgttgaagca cataatggcc gcattattgc tgcccattcg
    19741 ccttttggcg gggtaagcat tacagtagag ttaccgctgg aacgggattt acagagagaa
    19801 gtatgaccga gttaccaatc gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac
    19861 cgaagctggg gcagttgctc attgattatc tgcgtgctgc gagctatgcg ccgacgctta
    19921 tcagccacgg cgatcaggta ctgccgtatg tgcgccagac accaccggat ctgatcctgt
    19981 tggatctgat gctccctggc accgatggct tgacgctgtg ccgggaaatt cgtcgttttt
    20041 ctgacattcc tatcgtgatg gtgacggcaa aaatcgaaga gatcgaccgc ctgctcggac
    20101 tggagattgg cgcagatgac tacatctgca agccgtacag cccacgggaa gtggtagcgc
    20161 gcgtcaaaac cattttgcgc cgttgcaaac cgcagcgcga attgcagcaa caggatgccg
    20221 aaagcccgtt gattatcgac gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg
    20281 acctgacacc tgcggaattt cgtctgttga aaacgctctc tcatgaacca agaaaagtgt
    20341 tctcccgcga gcaattgctc aatcatcttt atgacgacta ccgcgtagta accgaccgca
    20401 ccattgacag ccacattaaa aacctgcgcc gcaagctgga atctctcgac gccgaacagt
    20461 catttatccg cgccgtttac ggcgttggtt accgctggga agccgacgcc tgccgcatcg
    20521 tttagtttta gcgacatgac tttgttagcc ggagatggcg ttcagaccag atccggcaac
    20581 attatcccac gcatggtcag caaactgaca ttatctctcc gccctggcag gcatcagaaa
    20641 atcgctcata ctttaatcgg taaacagcac ctttagatgc tgttttcgat acgcaatttc
    20701 aatcaaggag tcattatggc tggttggttt gaactcagta agagcagtga taatcagttc
    20761 cggtttgtgc taaaagcggg caatggtgag actatcctca ccagcgagct gtatacctca
    20821 aaagcctctg cggaaaaggg catcgcgtcg gtgcgtagca acagcccgca agaagaacgc
    20881 tatgagaaaa aaacggcaag taacggcaaa ttctatttca atctgaaagc cgctaatcat
    20941 caaattatcg gctccagcca gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc
    21001 tccgttaaag ccaatggcac aagccagacg gtgaaagaca atacgtaatt acgatgccgg
    21061 gcgtgggttg ttggcgtccg gctttatatc caaataacgc ttagttatta ataattaaga
    21121 gtaaccgccg ccattaaaat agtgggcact gacgagcact cgttgtgctt tctttgttta
    21181 ccatcccata cttatcctcc tgaacatgtt catgtgccaa aatatcttct aatactttcg
    21241 tgacgctatc cggtttatga catacactga tgtaagattt ttacttacag gcgggcgtaa
    21301 aatggccaga acgatgacgg ttgatctcgg cgatgagttg cgtgaattta tcgagtcctt
    21361 aattgaatcc ggcgactatc gcacccaaag cgaagtgatc cgtgagtcac tgcgattact
    21421 tcgagaaaag caagcagagt ctcgcctgca agcgttacgt gacatgctcg cagaagggtt
    21481 gagtagtggt gaagctcagc cgtgggaaaa ggacgcgttc ttacggaagg tgaaagcagg
    21541 gataaggaaa taatgtgcgt attattaaac tcatgccaaa agccaatgag gatttagaag
    21601 gcatctggta ttacagctat caccatttcg gcgaaccaca agccgacaga tatgtggaac
    21661 atctttcaga cgttctccag attctgagta acaataatat aggtacgccc agacctgaat
    21721 taggcgaagg tatttttgtt cttccttttg aacgccacgt catttatttc ctgcaatcac
    21781 caggggaaat tatcgtcatt cgtatcctta accaaaacca ggacgccacc agacatttac
    21841 actggagcta aacccgccga ttgcgctata atgcccgccc ttaaagtggg ggcactcccc
    21901 taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga attatgttta
    21961 aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg
    22021 gcgcagatgc tgtttatgcg ggccagccgc gttattccct gcgtgtgcgc aacaacgaat
    22081 tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg aaaaagtttt
    22141 atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc cgtgacctga
    22201 aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccgggg ctgattatgc
    22261 tggtgcggga gcacttccct gaaatgccga tccatctctc ggtacaggct aacgccgtga
    22321 actgggcgac ggtgaaattc tggcagcaaa tgggactgac ccgcgtgatc ctctctcgcg
    22381 agctgtcgct ggaagagatt gaagagatcc gcaatcaggt gccggatatg gaggtagaga
    22441 tcttcgtgca cggcgcgctg tgcatggcct actccggtcg ctgcctgctc tctggctata
    22501 tcaacaagcg cgacccgaac cagggcacct gcaccaacgc ctgccgctgg gagtacaacg
    22561 tccaggaagg gaaagaagat gatgttggca acatcgtaca caagtacgag ccgattccgg
    22621 tgcaaaatgt tgagccgacg ctgggtatcg gcgcaccaac cgacaaagtg tttatgatcg
    22681 aagaagccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac ggcacttaca
    22741 tcatgaactc gaaagatctg cgcgctatcg cccatgtaga acgcctgacc aaaatgggcg
    22801 tgcattcgct gaaaatcgaa ggccgtacca aatctttcta ctattgcgca cgcaccgcac
    22861 aggtttaccg caaagctatt gatgacgccg ctgcgggcaa accgtttgac accagcctgc
    22921 tggaaaccct ggaaggcctg gcgcatcgtg gctataccga aggtttcctg cgtcgtcata
    22981 ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc cagcagtttg
    23041 ttggtgagtt taccggtgag cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat
    23101 tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaat tttacccttg
    23161 agcacatgga aaacgccaaa ggcgaagcta tgccgatagc accaggcgat ggttatactg
    23221 tgtggctccc ggtcccgcag gatcttgagc tcaattacgc gctgctgatg cgtaatttct
    23281 ccggggaaac cacgcgtaat ccccacggta agtgattaat ttcgattatt tttcccggat
    23341 ggaaaattct tagaaaccga tcacatacag ctgtatttat taaggttatc atccgtttcg
    23401 ctgaaaaaca taacccataa aatgccagct gtaccaggaa ccacctcctt agcctgtgta
    23461 atctccctta cacgggctta tttttttatg cataagccct atctctggta accgtcttcc
    23521 attgaccaca tcgatagaat cctccttcat agcacgatgc ctttcactta tcggcatcgt
    23581 gctcccacag gttccggcta cgcacagcca gaacgcgcat atttgacgct taccaaaaaa
    23641 tattctcact ctccacattt gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct
    23701 gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg ggctgcatca
    23761 ttttttacta tattttctgc accagatatt ctgcccctgg ctaaagaagc ttcggcttcg
    23821 gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag accaaattta
    23881 ccgatacttg tctcatcatt atgcacagca caaccagaca taataaaaat actaattaat
    23941 gatatagcag ctatcttttt catctcacct tcccccatta aatacc
//
LOCUS       DAZFAH010000054        23467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFAH010000054 DAZFAH010000000
VERSION     DAZFAH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.054"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1162)
                     /locus_tag="ACHJWB_002045"
     CDS             complement(<1..1162)
                     /locus_tag="ACHJWB_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4313685.1"
                     /translation="MALTDAKIRAAKPSDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEE"
     gene            complement(1360..1435)
                     /locus_tag="ACHJWB_002046"
     tRNA            complement(1360..1435)
                     /locus_tag="ACHJWB_002046"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1400..1402),aa:Phe,seq:gaa)
     gene            complement(1541..2248)
                     /gene="yqgA"
                     /locus_tag="ACHJWB_002047"
     CDS             complement(1541..2248)
                     /gene="yqgA"
                     /locus_tag="ACHJWB_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4313686.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPAMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2401..2484
                     /gene="yqgH"
                     /locus_tag="ACHJWB_002048"
     CDS             2401..2484
                     /gene="yqgH"
                     /locus_tag="ACHJWB_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO4313687.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2646..4781
                     /gene="speC"
                     /locus_tag="ACHJWB_002049"
     CDS             2646..4781
                     /gene="speC"
                     /locus_tag="ACHJWB_002049"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4313688.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     RAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4831..6087)
                     /gene="nupG"
                     /locus_tag="ACHJWB_002050"
     CDS             complement(4831..6087)
                     /gene="nupG"
                     /locus_tag="ACHJWB_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4313689.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6289..7368)
                     /gene="mltC"
                     /locus_tag="ACHJWB_002051"
     CDS             complement(6289..7368)
                     /gene="mltC"
                     /locus_tag="ACHJWB_002051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4313690.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7433..7708)
                     /gene="yggX"
                     /locus_tag="ACHJWB_002052"
     CDS             complement(7433..7708)
                     /gene="yggX"
                     /locus_tag="ACHJWB_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4313691.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7736..8788)
                     /gene="mutY"
                     /locus_tag="ACHJWB_002053"
     CDS             complement(7736..8788)
                     /gene="mutY"
                     /locus_tag="ACHJWB_002053"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4313692.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHSG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFSDEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8949..9668
                     /gene="trmB"
                     /locus_tag="ACHJWB_002054"
     CDS             8949..9668
                     /gene="trmB"
                     /locus_tag="ACHJWB_002054"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4313693.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9668..9994
                     /gene="yggL"
                     /locus_tag="ACHJWB_002055"
     CDS             9668..9994
                     /gene="yggL"
                     /locus_tag="ACHJWB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4313694.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10178..10897
                     /gene="yggN"
                     /locus_tag="ACHJWB_002056"
     CDS             10178..10897
                     /gene="yggN"
                     /locus_tag="ACHJWB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4313695.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11073..12119
                     /gene="ansB"
                     /locus_tag="ACHJWB_002057"
     CDS             11073..12119
                     /gene="ansB"
                     /locus_tag="ACHJWB_002057"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4313696.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12236..13243
                     /gene="yggM"
                     /locus_tag="ACHJWB_002058"
     CDS             12236..13243
                     /gene="yggM"
                     /locus_tag="ACHJWB_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4313697.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13398..14534)
                     /gene="hemW"
                     /locus_tag="ACHJWB_002059"
     CDS             complement(13398..14534)
                     /gene="hemW"
                     /locus_tag="ACHJWB_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4313698.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14527..15120)
                     /gene="rdgB"
                     /locus_tag="ACHJWB_002060"
     CDS             complement(14527..15120)
                     /gene="rdgB"
                     /locus_tag="ACHJWB_002060"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4313699.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15128..15418)
                     /gene="yggU"
                     /locus_tag="ACHJWB_002061"
     CDS             complement(15128..15418)
                     /gene="yggU"
                     /locus_tag="ACHJWB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4313700.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15415..15981)
                     /gene="yggT"
                     /locus_tag="ACHJWB_002062"
     CDS             complement(15415..15981)
                     /gene="yggT"
                     /locus_tag="ACHJWB_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4313701.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(15999..16703)
                     /gene="yggS"
                     /locus_tag="ACHJWB_002063"
     CDS             complement(15999..16703)
                     /gene="yggS"
                     /locus_tag="ACHJWB_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4313702.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16721..17701
                     /gene="yggR"
                     /locus_tag="ACHJWB_002064"
     CDS             16721..17701
                     /gene="yggR"
                     /locus_tag="ACHJWB_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4313703.1"
                     /translation="MNMEEIVALTVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(17877..18293)
                     /gene="ruvX"
                     /locus_tag="ACHJWB_002065"
     CDS             complement(17877..18293)
                     /gene="ruvX"
                     /locus_tag="ACHJWB_002065"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4313704.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18293..18856)
                     /gene="yqgE"
                     /locus_tag="ACHJWB_002066"
     CDS             complement(18293..18856)
                     /gene="yqgE"
                     /locus_tag="ACHJWB_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4313705.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(18965..19915)
                     /gene="gshB"
                     /locus_tag="ACHJWB_002067"
     CDS             complement(18965..19915)
                     /gene="gshB"
                     /locus_tag="ACHJWB_002067"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4313706.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIGETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(19928..20659)
                     /gene="rsmE"
                     /locus_tag="ACHJWB_002068"
     CDS             complement(19928..20659)
                     /gene="rsmE"
                     /locus_tag="ACHJWB_002068"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4313707.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20739..21446)
                     /gene="endA"
                     /locus_tag="ACHJWB_002069"
     CDS             complement(20739..21446)
                     /gene="endA"
                     /locus_tag="ACHJWB_002069"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4313708.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21541..22038)
                     /gene="yggI"
                     /locus_tag="ACHJWB_002070"
     CDS             complement(21541..22038)
                     /gene="yggI"
                     /locus_tag="ACHJWB_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4313709.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22115..>23467)
                     /gene="galP"
                     /locus_tag="ACHJWB_002071"
     CDS             complement(22115..>23467)
                     /gene="galP"
                     /locus_tag="ACHJWB_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4313710.1"
                     /translation="TFFVCFLAALAGLLFGLDIGVIAGALPFIADEFQITSHTQEWVV
                     SSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAAPNVEVLILSRVLLG
                     LAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLSDTAFSYTGAWRWML
                     GVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTSAEAKRELDEIRESL
                     QVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPKIFELAGYTNTTEQM
                     WGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVLGTMMHIGIHSPSAQ
                     YFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTATNWIANMIVGATFL
                     TMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERNLMKGRKLREIGAHD
                     "
BASE COUNT         5774 a         5925 c         6183 g         5585 t
ORIGIN      
        1 gttcttccag atgttctgct ttatgaatgt aagcagcacg tacaccgtta cgttcctgat
       61 ggctcatctg acgttccaca gcatcgcggg accacaaacc tgattcaatc aatgcactgc
      121 acgccatcgc ccggaatcca tggccacaga cttcctgggt tgtgtcatac cccatcaccc
      181 tcagggcttt atttacagta ttttcactga ttggtttacg cgggtcatgt gcacccgtaa
      241 atatcagacc attttcacct gcccatgtct gaagttctgt cagtatggct acagcctgtt
      301 tgctgagtgg cacaagatgt ggtgttcgca tcttggagcc ccggtgtgag aattttacgc
      361 ctggcagagg ttcgcgttcg gctggaatag tccacatggc attgtcaata tcaatttcag
      421 accagcgggc gtatctgagt tcactggaac gaataaaaac cagtaaattc agcataactg
      481 ccagtcgggt gacaggctgg cctttataac cgtcaatacg cgacagaagt tcaggcaggc
      541 gtgaaagatc cagagcaggg cgatgctggc gttttaccgt ggtcaatgca ccagccatat
      601 ccagagccgg gttatatctg atgatgccag actgtacagc ataacgcatt acagcagata
      661 ttctttgctg cagacgactg gcgatttcat aaatttgttt agcttctgca gcacgaacag
      721 ggataagcag gtccggggta ttgagtgttg tgatatcccg gttgccgata aaggggaaaa
      781 cgtgtgtttc gaggcttttg agtaccttat cgctgtgtga ttgtgcccat tgtttattac
      841 tggcatgcca gcgtcgtgca atatgttcaa aagagagttg taaatcaggg actacttttt
      901 tagctctttt ctgttcgcaa gggtcaatcc cctctgaaat cagttttcgg gcctcatccc
      961 gttttgtacg agcttcggag agagaaactt ctggatacac accaagtgcc agagtcttct
     1021 ccttacccag aatacgataa cggagacgcc agtaacggga accattagga tgaaccagca
     1081 ggaacatgcc agccccgtca gtgagtttat aagccttgtc agagggcttt gcagctcgga
     1141 tttttgcgtc agtcagtgcc atagtacagc cttctgaatg cgttaaagag ggtacaaatt
     1201 ctgtcgaact ggggtatacc cgcaattata cccacacaat ggggttgatg tggggggaat
     1261 ctggttgagt tcggtagaat tgatttgtag gtagaacgct taaatcatgg tgttctgaaa
     1321 cgaaaaacgg acctccgtgg aggtccgttg atatgaattt ggtgcccgga ctcggaatcg
     1381 aaccaaggac acggggattt tcaatcccct gctctaccga ctgagctatc cgggcaacgg
     1441 ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac
     1501 tgcacaatct atcatcactt tgccgctatt gcacgcattg tcaggcaaac caggcggtcc
     1561 aggcggcgga aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca
     1621 ttttaatgcc acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga
     1681 agtctgccat catcgccggt gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg
     1741 ttaactggat gatcagtaat gggatactaa tcaccgatac cgcaataccc agcgagcagg
     1801 cgaagatcat cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc
     1861 cggtcatccc ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa
     1921 taatcgcgac ataattctga ataaaagatt catgcgctgg cttcttacgt gagtgacgaa
     1981 acagattttg tgctttggtg actgctgtat tgacaccttt ctccagcaaa caaatttcgc
     2041 caattagagc gccaagtagc gtagctaaaa ccatcgctgg aaggttggca catttcacca
     2101 ccagtaaaat accaatcccc agcgatgcca gaccaaaaat agatgtcatg gagacgcgga
     2161 tacgttccgg taagcgttgg ctgagcagtg cgccgagaac gccacccagt aatactgcac
     2221 tagcgttgat aaaagggccg atgaccacaa gagttcctgt taactgtatt gtttgatttt
     2281 cttattatga ccgtttggtg ccgtcgatgg cttgtttgtt cgcaaagtcc tggcttgcac
     2341 gctttagcga aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg
     2401 atgcacgcca tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cgtttccctt
     2461 tctatgttac tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat
     2521 cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat
     2581 atctcatggg gcagggtttt ccaccttgtc cggtattctt acttccccga aacgggtttg
     2641 cgcttatgaa atcaatgaat attgccgcca gtagtgaact ggtatcccga ctttctactc
     2701 atcgccgcgt ggtggcgttg ggagatactg attttacgga cgtcgcggca gtcgtcatta
     2761 ccgctgcgga tagtcgcagt ggcattcttg cgttgcttaa gcgcaccggt tttcatctac
     2821 cggtgttttt gtattccgaa catgctgttg aattacctgc gggcgttacg gcggtaatca
     2881 acggcaacga gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt
     2941 tgctgccacc gttttatgac acgctgacgc agtacgttga aatgggcaac agcacctttg
     3001 cttgccctgg acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt
     3061 acgatttctt tggtgagaac gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg
     3121 gcgatttgct tattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct
     3181 ttcatgccga taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga
     3241 cgaatgcgct gttaacgcgt ggcgatctgg tgctcttcga ccgtaacaac cataagtcga
     3301 atcatcacgg cgcgctgatt caggcggggg cgacgccggt ctatctggaa gcttcacgca
     3361 acccgtttgg tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc
     3421 agcaaattcg cgacgttgcg ccagaaaaag ccgagctgcc gcgcccgttt cgcctggcga
     3481 ttattcagct gggaacctat gacggaactg tctataacgc ccgtcaggtg atcgataccg
     3541 ttgggcatct gtgtgattac attctgtttg attccgcgtg ggtcggttac gaacagttta
     3601 tcccgatgat ggcggatagc tcgccgctgc tattagaact taacgaaaac gatccgggga
     3661 tctttgtgac tcagtcggtg cacaaacagc aggcgggatt ctcacagacg tcgcagatcc
     3721 ataaaaaaga taaccatatc cgcggacagg cgcgtttttg cccgcataag cggttgaata
     3781 acgcctttat gctccatgct tctaccagcc ctttctatcc gctgtttgct gcactggatg
     3841 ttaacgccaa aattcatgaa ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt
     3901 tgggtattga gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc
     3961 cgcccgttgt tgatggcaaa ttgtggcagg attatccgac atcagtgtta gccagcgacc
     4021 gccgtttttt cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg
     4081 atcagtattt tgttgatccg tgcaagctgt tactcactac accaggtatc aatgccgaaa
     4141 ccggcgaata tagcgacttt ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga
     4201 acggcattgt gccggagaag tgcgatctca actccattct gttcttatta actccggcgg
     4261 aaagccacga gaagctggca caactggtag agatgttggc gcagtttgaa cagcatattg
     4321 aggatgactc gccgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct
     4381 atcgcgacta cactttgcgc cagttgtgtc aggagatgca cgatctgtat gtcagtttcg
     4441 acgtcaaaga cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga
     4501 acccccagga tgcgcataga gcttatattc gcggtgaagt ggagttggtg cggattcgtg
     4561 atgccgaagg gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg
     4621 tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta tttccttgca ctggaagaag
     4681 gggtgaattt gttgccggga ttttcgccgg agctgcaagg tgtttatagc gaaaccgatg
     4741 cggatggcat gaaacggttg tacggttatg tgttgaagta agaataaaaa aaacgggtca
     4801 ccttctggcg acccgttttt ctttgcgtaa ttagtggcta accgtctgtg tgcctgtcgg
     4861 gacacgaacg tgtttatatt tgaacatcgc catgaacgcg aaggccagaa ccacggagta
     4921 accagcgaaa atcaaccata cggtctgcca gtcggtaatg ccgttttggg tgtacatctc
     4981 aacaacttta ccgctcacga tgccgccgag gatacagccg aagccgttag tcatcatcag
     5041 gaacatccct tgtgcactgg cgcgaattgc cgggctaact tctttttcga caaacaccga
     5101 accagagatg ttgaagaagt cgaatgcgca accgtaaacg atcatcgaca gtaccagcag
     5161 tacagtaccg aacggagtcg ggtcgccgta agcaaacagc gcaaaacgca ggatccacgc
     5221 cacaatactg atcatcatta cgttcttaat accgtagcgg cttaagaaga atgggatggt
     5281 cagaatgaac agggtttcag agatctgcga aatcgacatg atgattgacg catgctgcac
     5341 aataaagctg ctggcaaaca ttggatcttt gtcgaagctg tgcaggaagg tattaccgaa
     5401 catgttggta atctgcagtt ccgcgcccag cagcattgag aagataaaga agattgccat
     5461 acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc agggttgtcc agctctgatt
     5521 cgcttgctgt ttagcaacag gaatatgcgg cagagtcagg gtaaacagaa ccagaatggc
     5581 ggaaagtgct gcgccaatat acagctgcat gtggcttaat tcgaagccag acaggctcac
     5641 cacccacatt gccatgataa agccgatggt gccccagata cggattggcg ggaagtcagt
     5701 aacgatatcc atcccggcat tttgcaggcg atagtaagag atggtgttga ttaacccaag
     5761 cgttggcata taagcaaacg agttaatcaa tatcacaagg aacatcgctt ccggcgtcgt
     5821 gacctgtgcc gccatgaaca gcgtgatagc gccaatggtg tggcaaatgg catataccca
     5881 tttcgcactt aaccatttgt cggccacaat ccccagcagc gcaggcataa ataccgcggc
     5941 gatacccagt gagctataaa ctgcgccaat agaagcaccg tcaaacttca gggtaacaaa
     6001 catataggag ccgagggtcg tcagccaact tccccacaga cagaactgca gaaaagagag
     6061 gattttcagc tgcagcttaa gattcatgtt aatttcctca catcgtgatg cggatgagtg
     6121 tttgaaaaag cacatttgac cgtgcgagat gatgggattc tatcaacttc gcggactggt
     6181 tttttgttac ctgtggcaaa taattgcaaa cattcccaat ttgcaaaacg aaattgtgat
     6241 gtggataaca tttttgcccc tgagcatcgt caggggcggt taatggaatt atcggcggcg
     6301 gtaggatttt tgcgcggtat tcactttata aagataacgg cgagattccg cagagggatg
     6361 gcgggtcgtc agcgtctgat aaacatcgcc cggcgtcatg gtgttaataa tattggcagc
     6421 ctgaatctta tcattcgaaa agactcgcag cacgctgcct gcgccgccgt tataggcggt
     6481 gatgacggca taacgccgcg atgttgggtt atcaattccg ccgagataaa cattgttcag
     6541 catcgccaga tacgcggtgc cggtatcaat attgctggca ggatcaaaca agaaactgcg
     6601 gctcggcgtg ccggatttcc cctgcgagcg gaacacatct ttcccggcag tatgttgtac
     6661 cacctgcatt aatcccagcg catcggaacg gctgaccgca tatgggttaa aggaagattc
     6721 ggtctgcata atcgccagaa tcagcgactc atcaacgcca tatttccgtg acgcctggcg
     6781 gaccatgccg agatatttgt gcgcacgttt atcaaggtgg ttcggcacca tgttaatggt
     6841 aacgctgtag atgatacgca gcccgttgct gcggctcttc agacggtttt tcagcagata
     6901 atccgcgaag ttgctcgcgc gaccttccca gcgaatcggc tgcccggtgt tgtccaccac
     6961 ttgaccgtaa aggaaaggtt ctttcgaaat tgtaatatca tcaacgtcgg aatagagatc
     7021 gaccgaactc ggatcgtcac ccatcagcaa cgttttgata attgcccggc gcaaatgcgc
     7081 ggcaggttct gtcccggcga tggtttcgat agtaatcgta ccgtcatcga agttgatgtg
     7141 gctgcgggtc tgatattgat cggtgtattt cacgtagtcc ttaggaccag cgatcaccac
     7201 ctctttgaag ccccagatgt tctcaatatt gtgggcaaat tgccccatca gaatatcaaa
     7261 accgttggta tctttgaccc aggcttcgtt ataggtatcg ccttttttgg tcgtcgaaca
     7321 ggagatgagc aacggcgcaa tcaaagccag cgcgagatat tttttcatca ttccgggtgc
     7381 gtgttgtgtt tgtttttatg caagctgccg gaggcgtgct ccggcactgt ttttattttt
     7441 tatcttccgg cgtatagccc tcgatatgca cctctttacc ctcgaacagg aagttgacca
     7501 tctcctgctc cagcagcttg cggtgctcgg cattcatcat gttgagtttc ttttcattaa
     7561 tcagcatggt ttgtttgtgc tgccactgcg cccaggcttc tttggagatc tcgttataga
     7621 tgcgttttcc cagctcgccg gggtacagct gaaaatcctg accttctgct tcacgttgca
     7681 ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct ttatcgactc acgcgctaaa
     7741 ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac gggagccgcc aggccaactg
     7801 acggcggttg cgctaagtta taccagagcg cattgccttc atccatgcag ccggtgaatg
     7861 acgacacggg aagccacata ggcacaatat ctaagtggaa atggctgaaa gtatggcgaa
     7921 acgcggtcag ctgcgtcagg ttatcggcag caatctgccg ttgcgccagc caatgccgca
     7981 aactttcttc gtcggaaaac tgcgggaaac agtataaacc gccccacaat ccgctcggcg
     8041 gacgctgcgc cagcaatact tcatcttcgt gctgtaatag caaaaagtag ccggtgcgct
     8101 ccggcagcgt ctgtttcggt tttttgcccg gataaagcga ccagctattg ttggcggcgg
     8161 caatacatcc gttttgtagc ggacagagcg aacatttcgg tttcgagcgc gtacaaatca
     8221 tcgcgcccaa atccatcatc gcctgattaa accgttccac gccgaccgcg ggcgtcacct
     8281 gctcgcttaa actccataat ttattctcga cctctttttt cccaggccag ccgcttacag
     8341 catagcagcg cgccagcacg cgtttgacgt taccgtcgag aatcggaaag tgcttaccca
     8401 gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac gcccggtaac gccgcgactt
     8461 cttcaaaggt ttccgggaat ttaccgctgt gtaaggtcgc cacttgttgt gccgctttat
     8521 gcaggttgcg cgcgcgggcg taatagccaa gcccggtcca caagtggaga acttcgtcga
     8581 gcggcgcatt ggcgagatcg gtcaccgtcg ggaagcgcgc cataaagcgt tcaaaatagg
     8641 ggataacggt cgcaacctga gtttgttgca acatcacttc tgagagccat actttgtagg
     8701 gcgtcttgtc aatttgccag ggcagcgttt tccgcccgta tttatcgtac cagtccagaa
     8761 cctgggctga aaattgcgac gcttgcatgg tcaccgaatt cactgttgtt gggggcaaga
     8821 ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc gcagccacgg ccttatcatg
     8881 cttgcatccg gcaactaact ttggataatg cccgttttca gaacactttc acaagcgact
     8941 aaatctttat gaaaaacgac gtcatttcac cggaatttga tgaaaacggc cgcccactgc
     9001 gccgtatccg tagttttgtg cgccgccagg ggcgactgac caaaggccag gaacatgcgc
     9061 tggaaaacta ctggccggtg atgggcgttg agttcagcga agatatgctg gatttccccg
     9121 cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg ttttggcatg ggggcgtcgc
     9181 tggtggcaat ggctaaagat cgccctgagc aggacttcct cggcattgaa gtgcattcac
     9241 cgggcgttgg tgcgtgcctg gcttctgcgc atgaagaagg tttaagcaac ctgcgcgtga
     9301 tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc tgacaattca ttgcgcatgg
     9361 tgcagctctt tttccctgac ccgtggcaca aagcgcgcca taataaacgc cgtatcgtac
     9421 aggtgccgtt tgccgaactg gtaaaaagca aactgcagct ggggggcgta ttccatatgg
     9481 cgaccgactg ggaaccttat gcggaacata tgcttgaagt gatgtcttct attgacggtt
     9541 ataaaaacct gtcagagagc aatgattacg taccgcgtcc ggcatcacgt ccggtgacga
     9601 aatttgaaca acgtggtcat cgtcttggtc acggagtatg ggacttaatg ttcgagaggg
     9661 tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa aaaatgcaca tcgacgaatt
     9721 ccaggaatta ggattttcgg tggcatggcg attcccggaa ggtacatcgg aagaacagat
     9781 tgataaaacc gttgatgatt ttattaacga ggttatcgaa ccgaacaaac tggcctttga
     9841 cggcagcggt tatctggcct gggaaggtct gatctgcatg caggaaatcg gcaaatgcac
     9901 cgaagaacat caggcgattg tgcgtaagtg gctggaagag cgcaaactgg atgaggtacg
     9961 caccagcgaa cttttcgacg tttggtggga ctaagaaagc atacgggcga tgacaaatgc
    10021 aaaactgcct gatgcgctac gcttatcagg cctggaaaga tgcacgatcg agtaggcggg
    10081 ataaggtgtt tacgccgcat ccggcatgga aaacgcgtac tttgttatca atctggggcc
    10141 agcaaatgct ggcctgattt tttcttgagg gaagactatg atgcgcaaaa tgctgctggc
    10201 ggcagcactt tcagtgacgg caatgaccgc tcacgccgac taccagtgca gcgtcacgcc
    10261 gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg aagggcgaaa acggcaatct
    10321 ggtgatcacg ccagacggca acgtgatgta taacggtaag caatattccc tgaatgccgc
    10381 ccagcgcgag caggcgaagg attatcaggc tgaactacgc agcacgctgc cgtggattga
    10441 tgaaggcgcg aaaagccgcg tcgaaaaagc ccgtattgcg ctggataaaa ttatcgttca
    10501 ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc aaacttgatg cgcagctgaa
    10561 agagcagatg aaccgcatta tcgaaacgcg cagcgatggc ctgacgtttc actataaagc
    10621 cattgatcag gttcgcgccg aaggccagca attagtgaat caggcaatgg gtggaatttt
    10681 acaggacagc attaatgaaa tgggcgcgaa agcggtgttg aaaagcggcg gtaacccatt
    10741 acagaatgtg ctgggaagcc tgggggggct gcaatcctca atccaaaccg agtggaaaaa
    10801 gcaggaaaaa gatttccagc agtttggcaa agatgtttgt agccgcgttg tgactctgga
    10861 agatagccgc aaagccctgg tcgggaattt aaaataatcc tctattttaa gacggcataa
    10921 tactttttta tgccgtttaa ttcttcgttt tgttacctgc ctctaacttt gtagatctcc
    10981 aaaatatatt cacgttgtaa attgtttaac gtcaaatttc ccatacagag ctaagggata
    11041 atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt tttcaaaaag acggcacttg
    11101 ccgcactggt tatgggtttt agtggtgcag cattggcatt acccaatatc accattttag
    11161 caaccggcgg gaccattgcc ggtggtggtg actccgcaac caaatctaac tacacagcgg
    11221 gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca actaaaagac attgcgaacg
    11281 ttaaaggcga gcaggtagtg aatatcggct cccaggacat gaacgataat gtctggctga
    11341 cactggcgaa aaaaattaac accgactgcg ataaaaccga cggcttcgtc attacccacg
    11401 gtaccgacac gatggaagaa accgcttact tcctcgacct gacggtgaaa tgcgacaaac
    11461 cggtggtgat ggtcggcgca atgcgcccgt ccacgtccat gagcgcagac ggtccattca
    11521 acctgtataa cgcggtagtg accgcagctg ataaagcctc cgctaatcgt ggcgtgctgg
    11581 tggtgatgaa cgacaccgta ctggacggtc gcgatgtcac caaaaccaac accaccgacg
    11641 tagcgacctt caagtctgtt aactacggtc ctctgggata cattcacaac ggtaagattg
    11701 actaccaacg taccccggca cgtaagcaca ccagcgatac gccattcgat gtctctaagc
    11761 tgaatgagct gccgaaagtc ggcatcgttt ataactacgc taacgcatcc gatcttccgg
    11821 ctaaagcact ggtagatgcg ggctatgatg gcatcgttag cgctggtgtg ggtaatggta
    11881 acctgtataa atccgtgttc gacaccctgg caaccgccgc gaaaaacggc actgcagtag
    11941 tgcgttcttc ccgcgtaccg acgggtgcta ccactcagga tgctgaagtg gatgatgcga
    12001 aatacggctt cgtcgcctct ggcacgctga acccgcaaaa agcgcgcgtc ctgctgcagc
    12061 tggctctgac gcaaaccaaa gatccgcagc agatccagca gatcttcaat cagtactaat
    12121 cacctcgccc cggtatcgtg ccggggcttt ttcacttcag acttacgtcc attgccaatt
    12181 ttcattaccc taatgataat caccggaata aattattccg cgtgaggttt ttcgggtgaa
    12241 aaagcaatgg attgttggca cggcactgct tatgttgatg actggtaatg cctgggcaga
    12301 tggcgaaccg ccaactgaaa atatcttaaa agatcaattc aaaaagcagt atcacggcat
    12361 tctcaagctt gatgctatca ccttaaaaaa tcttgatgct aagggtaatc aggccacctg
    12421 gtcagcggaa ggcgatgtct cttccagtga cgatctctat acctgggtcg gtcagttggc
    12481 agattacgaa ctgctcgaac agacctggac gaaagataaa ccggtaaaat tctcggcgat
    12541 gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg aacttttact cttttcaggc
    12601 ggcagccagc gatcgtgggc gggtggtcga cgatatcaaa acgaataata aatatctgat
    12661 cgtgaatagc gaagatttca attatcgctt tagtcagctt gaatctgcgt tgaataccca
    12721 gaagaactcg attcctgcgc tggaaaagga agtgaaagcg cttgataagc aaatggtggc
    12781 ggcgcagaaa gcagcggatg catattgggg aaaagatgca aacggtaagc aaatgacccg
    12841 cgaagaagcc tttaaaaaaa ttcaccaaca gcgtgatgag tttaataaac agaacgatag
    12901 cgaggcgttc gcggttaaat atgacaaaga ggtttatcaa ccggcgattg cggcatgtca
    12961 taaacagagt gaagagtgtt atgaagtgcc gattcagcag aaacgagatt tcgatatcaa
    13021 cgaacaacgg cgacagactt ttctgcaatc acaaaaactc agccgtaaat tgcaggatga
    13081 ctgggtaacg ctcgaaaaag ggcaatatcc gttaacgatg aaagtttcag aaataaacag
    13141 taaaaaagtc gcgattttga tgaaaattga cgatatcaac caagctaatg aacgctggaa
    13201 aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag taagtttgat atagtgcacg
    13261 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgctt
    13321 gcgcgtctta tcagacctac aaccgttgcc acatgtaggc tggataaggc gttcacgccg
    13381 catccggcaa tacaagttta ctcagccaga aaaagctcca gcagcgaatt taaaaacagc
    13441 ttcccatgtt ccgttatctg ccagtaatcc gcacattctg tgagataacc ctgggcaatc
    13501 gcctcgtcta actgtgggcg aatcacatct tcgcaaagcc cggtatacgc aataaactcc
    13561 acgcgcggcg cggcctccag cagacggaag cgattcataa agaactcaaa cggcttatct
    13621 gcggcttcga catcacgctg gctttccaga taccttcctt gcataaaacc acgcggatga
    13681 cgcgttttag tggtacgcag aatgcgccca tccgggaagg tcactttgcc gtgcgcgccg
    13741 cagccaatac cgatgtagtc gccaaagcgc cagtagttga gattgtgctg gcactgataa
    13801 ctgggtttgg cgtaagcgga agtttcatat tgctgataac ccgctgcggt taataactga
    13861 tgcccctgtt cgaatatatc ccacagcgcg tcgtcgtccg gcagtaccgg cgggcgtgaa
    13921 ccaaacagcg tattaggttc gatggtcagt tgataccagg aaagatgcgg cggattcagt
    13981 tcaatggcct ggcgtagatc gccaagcgcc tcttccagtg attgatccgg tagtccatgc
    14041 atcaaatcga ggttaaagct acgcagcccc agcccgctcg ccagctttgc cgcgcgtttc
    14101 gcttcttgcg ggccatgaat acgcccaagt cgtttcagct tgtcttcgct aaaactctgc
    14161 acaccaatag agatgcggtt cacaccagca cgctgataat cgacaaagcg atcggcttct
    14221 accgtaccag ggttcgcttc catagtaatt tctgcatccg ctgccagcgg caaacgcgca
    14281 cgcacgccgt ccagcagcgt ttgcatcgcc gggccggaaa gcaggctcgg cgtaccaccg
    14341 ccaataaaga ttgtctttac ttcacggccc tgagcgtaag ccacatcgtt gtccagatcg
    14401 caaagcagat gctgaacgta atcgtcgtgc ggcacttctc ctttcaacgc gtgagagttg
    14461 aaatcgcagt acgggcattt ctgcacgcac cacgggatgt gaatgtagag actcagcggc
    14521 ggtaatttaa ccattacgta aagcgtccaa cagcagtttc aatgcctgac cacggtggga
    14581 aatggcgctc ttttcttcgc gggtcagttc ggcagcggtt ttcccttcgg aaggtacgaa
    14641 gaagattgga tcataaccaa agccaccagt gcccgctggt tcacgagtaa tcacgcccgg
    14701 ccagctaccg tggcacacca gcggagtcgg atcttccgcg tgacgcagat ataccagcac
    14761 gcagtggaaa cgcgcctgac gttggtcgtc cggtacgtct ttcagtgttt ccaacagttt
    14821 ttgcagattc ttttgatcgg tcgcgtcttc accggaataa cgcgcggagt aaatacccgg
    14881 cgcgccaccc agcgcatcta ccgccagacc agagtcgtcg gcaattgccg gtaaaccggt
    14941 cacttttgcc gcatggcgcg ctttcagaat cgcgttttcg ataaaggtca ggccggtttc
    15001 ttcagcggaa tctacgccga ggtctgtttg ggccacgata tcaagaccga agtcgctaag
    15061 cagcgacgcc agctcacgca ctttaccggc attgccggtt gcgaggacaa ctttttgcat
    15121 aggataccta attaattaac gccgcgattt ctggcgggat ttgttgcgga ttaatgattt
    15181 taatttgttt gtggcggcca agttcgcctt tttcaattac cacctggctt ttggctaccc
    15241 ggaattgctt accgagaaac ttcaccagat ggctgttggc ctggccgtca accggcggcg
    15301 cggtaatggc gactttaact tcgtcgccat gtaaaccgac aatagaatca cggctggctt
    15361 tcggctgaat atagagccgt aaaaccagac cgtcatcatt aactgttacg gcactcataa
    15421 cgccatccac agccccggca gcagcatgtt tccggttgcc tgtaatactt ctgcgacacc
    15481 catattgatg acatacagca gcagaacgag gatcatcggc gagaaatcaa tcccacccat
    15541 tgccggtagc aggcggcgaa tcgggcgcag cagcggatcg gccagctgaa tcagcacgta
    15601 ttcaatcgga ctacgaccct ggcttaccca gctcataatc gccatcacca gcaggaccca
    15661 gaaaatcagc aggccgatgg ttttcagcag aatcagtaaa ccggcaatcc agatgattgg
    15721 caggaaggtc accactttaa acagcacgat ggctttgata aaactgagaa tgtacgcaac
    15781 cagcagcgag gcgctatcaa tgggacccat tgccggaata acgcggcgca gtggcccgat
    15841 aattggctgt gttaccttca ctacaaactg tgagaagggg ttgtaaaaat cacaatgagc
    15901 ccactgcatc cagatgcgta ataacagcac catggtatac agctcaatga ccgttgaaag
    15961 caggaaagtc aacgtattca tggcgttcct ttaattcctt attttttaga gtaatcacgc
    16021 gcaccaaaaa ttgcagtgcc gatacgaacc atcgtgctac ctgccgcaat ggcggcttcc
    16081 atatcgtccg acattcccag agagagcgtg tcgatatgcg ggtagcgcgt tttcagtccg
    16141 gcaaatgcta cagccatttg gcgtgcaact tcaaactgcc ttacatattc tgactcaggc
    16201 gcagggattg ccatcagccc gcgcagacgt aaacgcggta gttcagcgac cgccgctgcc
    16261 agctcgtcca gttctgccag ttgaatcccg gacttactgt tttcatcact aatattaatt
    16321 tgaatcagta cgttaagagg gggaagttct gccgggcgct gatcgttgag gcgggtagca
    16381 atgcgcaaac ggtcgatggt atgacaccag tcgaaatgct ctgccaccag gcggctttta
    16441 ttagactgca acgggccaat aaaatgccat tctaatcctg ttacgcccag ttcttgaaag
    16501 tggcgaattt tatctacccc ttcctgaacg tagttttcac caaattgacg ctgcccggca
    16561 tcaatggctt ctgcgatggc gctcgcaggt tttgttttac tgactgcaag cagcgtaatt
    16621 tcttctggag aacggccgca acgcgttgca gccgctgaga ttttgtcccg gacctgtgcc
    16681 aggttatgcg caatatcgtt cattttccga ggatcttagt atgaatatgg aagaaattgt
    16741 ggcccttact gtaaagcata acgtctcgga tctacacctg tgcagcgcct ggcccgcacg
    16801 atggcgcatt cgcgggctaa tggaagctgc gccgtttgat gcgccggacg tcgaagagct
    16861 actgcgggag tggctggatg acgatcagcg ggcaatattg ctggaaaatg gccagctgga
    16921 ttttgccgtg tcgctggcgg aaaaccagcg gttgcgtggc agtgcgttcg cgcaacggca
    16981 aggtatttct ctggcattac ggttgttacc ttcgcactgt ccacagctcg aacagcttgg
    17041 cgcaccaacg gtattgccgg aattactcaa gagcgagaat ggcctgattc tggtgacggg
    17101 ggcgacgggg agcggcaaat ctaccacgct ggcggcgatg gttggctatc tcaatcaaca
    17161 tgccgatgcg catattctga cgctggaaga tcctgttgaa tatatctatg ccagccagcg
    17221 atgtttgatc cagcagcggg aaattggttt gcactgtatg acgttcgcat cgggattgcg
    17281 ggccgcattg cgggaagatc ctgatgtgat tttgctcgga gagctgcgtg acagcgagac
    17341 aatccgtctg gcactgacgg cggcagaaac cgggcatttg gtgctggcaa cattacatac
    17401 gcgtggtgcc gcgcaggcag ttgagcgact ggtggattca tttccggcgc aggaaaaaga
    17461 tcccgtacgt aatcaactgg caggtagttt acgggccgtg ttgtcacaaa agctggaagt
    17521 ggataaacag gaaggacgcg tggcgctgtt tgaattattg attaacactc ccgcggtggg
    17581 gaatttgatt cgcgaaggga aaacccacca gttaccgcat gttattcaaa ccgggcagca
    17641 ggtggggatg ttaacgtttc agcagagtta tcagcagcgg gtgggggaag ggcgtttgtg
    17701 acgttgccat gcccgatgcg caagtttata gggcctataa aggcaataaa tgtctgatgc
    17761 gacgctaacg cgtcttatca gacctacagc cgctgccaga tgtgtgctgg ataaggcgtt
    17821 cacgccgcat ccggcaagat ggcggtaacg actatccgcc aggcttttag atcgccttaa
    17881 tatccctgct cgaaatagct ttcgagaata ataaccgcag aggcagagtc gactttgcct
    17941 ttgttgagcg cccgatagcc gccctgttca aacagaccgg aacgggcttc cacggtgcta
    18001 agacgttcgt catggagctt tacttcaaca ccgaaacgac catgaatacg gttggcaaat
    18061 ttacgcgctc tggcagtcaa tggttgctcg gtgccgtcca tattcagcgg caaaccgacg
    18121 atgatttcgt ccggctgcca ctctttcagt aaacgttcga tgaggttcca gtccggcgta
    18181 ccgtcctgtg ctttaattgc aggcaaaggg cgagcagtgc cggtaatgcg ttggccgacc
    18241 gctacgccaa tgcttttggt gccgaagtcg aaggcgagta aggttccact catcaggcgt
    18301 gccctgccac accaggcatg gtgagaatat ccacaccaat cagttttgcc gcctcgcgcc
    18361 agcggtcggc aatcggcgtt ttgaacagaa tattcagatc cgccggggcc gttagccacg
    18421 cgttatcgag aatttcttgt tccagttgac ctttttccca ggaggcataa cccagagcca
    18481 ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc cagcacatcg cgggaagtgg
    18541 tcatcactgt gttgtctgaa atgcgaatgc tggaagcaaa attggagggc ggagtatgca
    18601 aaataaaccc gcgatcttca gccagcggac cgccgagcat aaccggttta tccagacgga
    18661 ttgattcatc acgcggctcc ggcgtgatct tcagcttttc cagaatccct tcaattttga
    18721 gattttccag cggcttgttg acgatgatcc ccattgcacc attggtatta tgttcgcaaa
    18781 tgtagaccac ggaacgacgg aaaatcggat cctggagagc aggcatggca ataagaaagt
    18841 gatgctgtaa attcattgtc agaggttctg tttcctggtt caaaaaagca acagcgccca
    18901 gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa cgagatcctt ctcgctaagg
    18961 tgggttactg ctgctgtaaa cgtgcttcga tggcatccat taacattccg gtgatcgaca
    19021 ccggaaactc tgcttcaatc tcacgaatac aggttgggct ggtgacgtta atttcagtca
    19081 gacggtcgcc gatgatatcc agaccaacaa aaatcagccc tttttctttc agcgtcggcc
    19141 cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg acgaggttca ccgcgaccac
    19201 cggcagccag attgccacgg gtttcgcccc cctgcggaat acgcgccagg cagtacggta
    19261 ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc tttaatggct ggcaggtaat
    19321 tttgcgccat gcagtagcga gtgccatgct cagtcagggt ttcgccaatc acgccgaggt
    19381 ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc cataccgtcc agcggcttaa
    19441 gaatgatgtc gctgtgtttc tcccagaacg cttttagctg cgctttattg cgcgtaacca
    19501 gcgtttctgg cgttaagtca gagaaccagg cggtaaacag tttctcgtta cagtcgcgca
    19561 ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc ggcacgttcc agaatatagg
    19621 tcgcgtagat aaactcggta tcaaacggcg ggtctttacg catcaggatc acatcgagat
    19681 cggccagcgg cagatcctgt tcaccgacga atgaaaacca ctcttcgtag ttctgcttca
    19741 cgttcagcgt gcgggtatgg gcgcgggctt cgccattgat cagatacaga tcgcccatct
    19801 ccatatagtg aagttcgtaa ccacgacgct gtgcttccag caacatagcg aaactggaat
    19861 ctttcttgat gttgatgttt gcgatggggt ccatcacgat gccgagcttg atcattattc
    19921 ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc ggtaatggcg gtgagcgcag
    19981 ttgtctctgt acgcaaaacg cgaggtccca acaggatatc agtaaattga tagcgggcag
    20041 tcatggcaat ttcatctgcc gataaaccgc cttccgggcc aatcagcagg cggacgcgtt
    20101 caaccggtaa cggcaacgta ttgatgctgt tactggcgcg cgggtgaaga ttcagtttca
    20161 gtccttcatc ctgctctgca caccaggctt ccagatccat cgctgggcgg atttccggca
    20221 cccggttacg accacactgc tcacaggcag caattgcaat cttctgccac tgctggagct
    20281 tcttgttcag acgttcacta tccagtttaa cgccgcagcg ctcagaaaaa agtggcgtaa
    20341 tgaggcttac accgagttcg atcgatttct ggatcgtaaa ttccattttt tcaccacgcg
    20401 acatcacctg accgaggtga atatgcagcg gagattcgcg atcgtcgatc ttgccttcca
    20461 gcaccttcac ttccacgctt tttttgctgg cgctggtaat ttcggcgtca aagacctggt
    20521 tgctaccgtc aaacaattgc aacgcctgcc ccggccccat gcgcagtacg cgcccgatat
    20581 ggttggcggc atcttcgcaa agcgcgatgt gagaatggct ggtcagtggt tcaggatgat
    20641 aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct ccccacgcgg ttgtacgcgt
    20701 ggggtagggg ttaacaaaaa gagtcccgct agtgtaggtt agctctttcg cgcctggcaa
    20761 gcgcgttgca catacgggtt atgattgcct tgcaccttcg cgatgcgttc atcgcgctcg
    20821 cactcccagt cggtaaccgg atacatcttg ttccatgcgt tgaacagctg cgtttgctgg
    20881 cgagagagtg tcaggttgta ttggtcgcgc atatagaagt aggtgcgcgc aatggcaccg
    20941 cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat cgaccttcat ggcgcattga
    21001 ccgtactggc cttcgccgcc attccactgg ctgtacataa agttgccgcg atcgccattc
    21061 acctcaccga ctgacggctg caggttatgc atatcgcttt ccatcttgcg atagaccgga
    21121 tctttagcgc agtttttacg tccaccgtcc tgccagcact ggcgctggtg accgaactgc
    21181 caggcgggaa cgacgtgttc ccactctacg cggctggctc ggttttcatt tttgcgcacc
    21241 tgatagccgc acgattgcag atcaacaacg ccttttttgc cctgccagtt aattttacat
    21301 ccgcaataaa acgtaccggg cgcgtcagcg tggactttta ccgccgcggc tttcgcctga
    21361 gaaaaactat tgataccttc ggccaacgcc gggccggaaa atgctgcgct cagtaccacc
    21421 gcagcaatag acaaataacg gtacatcact tactccgtgt taaaacgagc cagcaacgta
    21481 gcgaaagttg ttttggtatg caatcagcca gatcaggaaa gttcctgata gttcagatgg
    21541 ttatttcgca accagctgtt caccgcagtg aacacagcga tagacggcct cgccacgaac
    21601 tacgcgatta tggcggcgta cggtaagctg atgctcctgg cacttgcagc ggtaggggaa
    21661 ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta cgacgggcgg gaacgcccag
    21721 cacgctttcc atcatccact tccactcttt gccatgtggc gctacgcggc cgaaatgttt
    21781 ccataccagc aaatgtgcca gttcgtgcgg taccacttct tcaataaaag cttcactgtt
    21841 ttccaacagc aaaacgggat tgaggcggat ttcatagctt tccagccagg ctgttccggc
    21901 ggaggttccg cgctgggtgt aagagagttt tggctccggg tagttacgcc ctagcttcag
    21961 gttggcctgg gcgagttttt cccgcaggcg gcgcataacg gcctgctgga tggcgatagg
    22021 gagacgggat gttttcatag cggcagagga tagagcgaag aaaagatgac tgcaagaggt
    22081 ggcttcctcc gcgatgggag gaagcctgag gagattaatc gtgagcgccg atttcgcgca
    22141 gtttacgacc tttcatcaga ttacgttcaa tatgttccag cgaaacgtgt ttggtttccg
    22201 gtaccagcca caatgtcagc aggataaaca gtacgttcag agccgcatac acccagaagg
    22261 tgttggcgtt acccagcgtg ttgagcatgg tcaggaacgt tgcgccaacg atcatgttgg
    22321 caatccagtt ggtggcggtg gagcaggtga tgccaaaatc gcggcctttc agcggctgaa
    22381 tttcggagca cagtacccaa atcagcggac cggcactcat ggcaaaaccg acaataaaca
    22441 tcagcagcat ggcgatggcg aaatactgcg ctgacggaga gtgaataccg atatgcatca
    22501 ttgtaccgag tacgcccatg ccagcagcca tcaccaggaa gcccagcgtt agcgttggtt
    22561 tacgtcccca gcggtcaaca aggccgattg cgataaaggt ggcaagtacg ttggtcaggc
    22621 cgacaatcac ggtcccccac atttgctcgg tagtgttggt ataacccgcc agttcgaaga
    22681 ttttcggcgc gtaatacatg atgacgttca tcccggtgaa ttgctgcatt acctgcaaca
    22741 gtacgccaag gaacaccgcg cggcggaagt tgctgttctc tttaaacagc gcccagccac
    22801 tctgtttaac ctgcaaactt tcacggattt catccagttc gcgtttcgct tccgcgctgg
    22861 tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa acggcgtttg gcggcaaacc
    22921 aacgtgggct gtctggcagg aagaagacac caatcagcag caaaattgcc gggatgataa
    22981 tcacacccag catccagcgc catgcaccgg tgtagctgaa ggcggtatca gaaagataag
    23041 caccgaggat cccgatagtg atcatcaact gatacatcga gatcatactg ccacgaattt
    23101 tttccggcgc aatttcagag aggtacagcg gtgcggtata agaggccaca cccaccgcca
    23161 gccccagtag aacgcgggaa agaatcagta cttcaacgtt tggcgcagcc gcagagaaca
    23221 gcgaaccggc aacaaacaaa attgcgccga tcatcaggct ctttttgcgc ccgagtttaa
    23281 aggagagcca gccgctgccc accgcaccga ctgccgcacc gaacatcatg gagcttacga
    23341 cccattcttg cgtatgcgaa gtaatctgga attcatctgc aataaacggc agtgcgccag
    23401 caattacacc gatatccagg ccaaagagta atcccgccag agcggcaagg aagcagacga
    23461 aaaacgt
//
LOCUS       DAZFAH010000055        23292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFAH010000055 DAZFAH010000000
VERSION     DAZFAH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.055"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..3915)
                     /gene="entF"
                     /locus_tag="ACHJWB_002072"
     CDS             complement(34..3915)
                     /gene="entF"
                     /locus_tag="ACHJWB_002072"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4313711.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHVDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3912..4130)
                     /gene="ybdZ"
                     /locus_tag="ACHJWB_002073"
     CDS             complement(3912..4130)
                     /gene="ybdZ"
                     /locus_tag="ACHJWB_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO4313712.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4133..5335)
                     /gene="fes"
                     /locus_tag="ACHJWB_002074"
     CDS             complement(4133..5335)
                     /gene="fes"
                     /locus_tag="ACHJWB_002074"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO4313713.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5578..7818
                     /gene="fepA"
                     /locus_tag="ACHJWB_002075"
     CDS             5578..7818
                     /gene="fepA"
                     /locus_tag="ACHJWB_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO4313714.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7858..7956
                     /locus_tag="ACHJWB_002076"
                     /pseudo
     CDS             7858..7956
                     /locus_tag="ACHJWB_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7993..8613
                     /gene="entD"
                     /locus_tag="ACHJWB_002077"
     CDS             7993..8613
                     /gene="entD"
                     /locus_tag="ACHJWB_002077"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4313715.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8735..8887)
                     /gene="hokE"
                     /locus_tag="ACHJWB_002078"
     CDS             complement(8735..8887)
                     /gene="hokE"
                     /locus_tag="ACHJWB_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4313716.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8856..9125
                     /locus_tag="ACHJWB_002079"
     CDS             8856..9125
                     /locus_tag="ACHJWB_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313717.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9341..10459
                     /gene="ybdK"
                     /locus_tag="ACHJWB_002080"
     CDS             9341..10459
                     /gene="ybdK"
                     /locus_tag="ACHJWB_002080"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4313718.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10525..10773
                     /gene="ybdJ"
                     /locus_tag="ACHJWB_002081"
     CDS             10525..10773
                     /gene="ybdJ"
                     /locus_tag="ACHJWB_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4313719.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10838..11206
                     /gene="ybdF"
                     /locus_tag="ACHJWB_002082"
     CDS             10838..11206
                     /gene="ybdF"
                     /locus_tag="ACHJWB_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4313720.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11300..11953
                     /gene="nfsB"
                     /locus_tag="ACHJWB_002083"
     CDS             11300..11953
                     /gene="nfsB"
                     /locus_tag="ACHJWB_002083"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4313721.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12061..13308
                     /gene="ybdG"
                     /locus_tag="ACHJWB_002084"
     CDS             12061..13308
                     /gene="ybdG"
                     /locus_tag="ACHJWB_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4313722.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13376..14752)
                     /gene="pheP"
                     /locus_tag="ACHJWB_002085"
     CDS             complement(13376..14752)
                     /gene="pheP"
                     /locus_tag="ACHJWB_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4313723.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14854..17997)
                     /gene="cusA"
                     /locus_tag="ACHJWB_002086"
     CDS             complement(14854..17997)
                     /gene="cusA"
                     /locus_tag="ACHJWB_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4313724.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTFDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18009..19232)
                     /gene="cusB"
                     /locus_tag="ACHJWB_002087"
     CDS             complement(18009..19232)
                     /gene="cusB"
                     /locus_tag="ACHJWB_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4313725.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMALVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19248..19580)
                     /gene="cusF"
                     /locus_tag="ACHJWB_002088"
     CDS             complement(19248..19580)
                     /gene="cusF"
                     /locus_tag="ACHJWB_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4313726.1"
                     /translation="MKKALQVAMFSLLTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19604..20986)
                     /gene="cusC"
                     /locus_tag="ACHJWB_002089"
     CDS             complement(19604..20986)
                     /gene="cusC"
                     /locus_tag="ACHJWB_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4313727.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21143..21826
                     /gene="cusR"
                     /locus_tag="ACHJWB_002090"
     CDS             21143..21826
                     /gene="cusR"
                     /locus_tag="ACHJWB_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4313728.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21816..23264
                     /gene="cusS"
                     /locus_tag="ACHJWB_002091"
     CDS             21816..23264
                     /gene="cusS"
                     /locus_tag="ACHJWB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4313729.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIPPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5587 a         6363 c         5943 g         5399 t
ORIGIN      
        1 ttctgtaatt atgggtttat agatattatt aatttacctg tttagcgttg cgcgaataat
       61 cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg
      121 atagatatcc agctccgcaa tccacggcga ccaggcacgt tcgggactca taccttcctg
      181 aagtgtgcgc tcagcaacaa ataacgttgc ttttccatca aacggaacgc tatgggcggt
      241 ggttaacaag cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
      301 agtacttccc tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct ccgccagcac
      361 ttccgggtcc agaccattag cctctttttc ctgccagttt tgcgtctctg gcggccaggt
      421 atccagcaaa ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatacc
      481 ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg
      541 ctcaagtaac gttgccagat gcgcttcgca gacttcatcc aggtttgtcg ctgtctgcat
      601 gggtccatga gggcgcggcg actgaatgcc aataatcgac cattgtggat cgagataacg
      661 cgagagcacg ctaaactgcc aggcaaaacc ggacgccgga tggaaacaaa acagcgtcgg
      721 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg agctgtcctc
      781 ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg
      841 cgtcacctga cgagcaaact gtcgacttaa ctgcgctgcc agtttcattg ccagtagcga
      901 atgaccgccc agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
      961 cgcgaatgcc gcggcgataa tcgtttcact gcccgctttc ggtgcacgtc ccgacgcttg
     1021 tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagcgg
     1081 caactgcgga agttgcagca gaacgaccgg caccatatgc ggcggcaatg tttcgcgaag
     1141 ctgtgcctgt aatgcgctgg tatccaacgg cagtcccgat tgcgacacca gatagcccac
     1201 caactgacgc gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac
     1261 tgcctgttcg acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg
     1321 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc
     1381 cagccagcgg gcgacgtctc cggtacgata catccgttca cctggggcaa aaggatcggc
     1441 aataaaacgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttggatgcc
     1501 ggtgagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat
     1561 acgcagaccc gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc
     1621 cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata
     1681 caggttatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat cagccggtaa
     1741 ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag cggtttgcgg
     1801 cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc
     1861 atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg gttcagccat
     1921 caccagtttt gcccctgcga taaacggcca gaaaaactcc cacacagaga catcaaaact
     1981 gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat tttgcatcca
     2041 taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
     2101 gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca gcggcgcact
     2161 gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa catcgctaaa
     2221 gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt ccagcatcat
     2281 tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac
     2341 tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg ccacgctgtc
     2401 ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
     2461 ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
     2521 cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt
     2581 gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag
     2641 cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa
     2701 cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat caacgtgttc
     2761 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg
     2821 cgtctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc
     2881 gaacagcggt tcctctcctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta
     2941 acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag
     3001 cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg gtccgctagc
     3061 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcat agtccatgcg
     3121 attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg tacgctgcac
     3181 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
     3241 cagaatatct gccgaagcgc tgcgccccgc taaaggtgcc ggagaaagtg acgcgggcgg
     3301 cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc
     3361 gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt
     3421 tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgagtaa tggccgggaa
     3481 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc
     3541 aacatgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca aatcttgttg
     3601 caaatccgcc agcattaatg cctgcgcagt accgtgcgga tcaatattgg ttcgcaggtc
     3661 gataatttct ggcagttcga atatcagcgc atcatcgacc cactgccaga cttcgccgtt
     3721 atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccggcaa ccaccgcgcg
     3781 ggccagtaat ggcgcatcaa catctccagt taactcaacg taatgcgcca cgctccaggc
     3841 ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
     3901 taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tggccggtgt cagagtacgc
     3961 cagtggactt ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaatgtcc
     4021 cagcctgccg gtaaggcgca ttgttgtggc cacagactga attgcccctg cgcattgcgc
     4081 aaaatgtaaa acgctccctg tggatcatcg aagggattac tgaatgccat attcaactcc
     4141 tgtcatggaa aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac
     4201 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta
     4261 attgcgcata cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca
     4321 gcacaatgcg cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc
     4381 cctcttgctg cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacagc
     4441 caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc
     4501 cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta
     4561 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
     4621 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg
     4681 tcagcgaagt cagcacaggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca
     4741 aaactgccag cggacgttct tctgctgttg catcgccggt ggtaaaaatc catacacgac
     4801 gtgaattttt caaccgttca cttttccaga taatttcttt ggctgctatt tctggcgctt
     4861 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
     4921 gccctcgccc gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata
     4981 gttttcgcca gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat
     5041 cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc
     5101 actgccagac atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat
     5161 gatcggtcac accagtgatg tagatccata cgcgctttat cgtcgagtat tcttcagaac
     5221 cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt
     5281 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
     5341 aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg
     5401 ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acatttatta
     5461 accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
     5521 ttgaggacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
     5581 aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag
     5641 gcacaagagc cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc
     5701 gagcagaact tacaggcgcc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac
     5761 cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt
     5821 aactccacca gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg
     5881 gaaaacacgc tgattttgat tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag
     5941 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt
     6001 gaacgtattg aagttctgcg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc
     6061 ggcgtggtta acatcattac caaaaaaggc agcggtgagt ggcacggctc ctgggacgct
     6121 tatttcaatg caccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagcctg
     6181 accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag
     6241 gctgacgcgt gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg
     6301 acgttaccag ccgggcgcga aggggtgatc aataaagata ttaacggcgt ggtgcgctgg
     6361 gacttcgcac cattgcaatc gctggaactg gaagcgggtt acagccgcca gggtaacctg
     6421 tatgcgggcg atacccagaa caccaactct gacgcttaca ctcgctcgaa atatggcgat
     6481 gaaaccaacc gcctgtatcg ccagaactac gcgctgacct ggaacggtgg ctgggataac
     6541 ggcgtgacca ccagcaactg ggtgcagtat gaacacaccc gtaactcgcg tattccggaa
     6601 ggtctggcgg gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat
     6661 atcgatctcg atgacgtgat gctgcacagc gaagttaacc tgccgattga tttcctcgtt
     6721 aaccagacgc tgacgctggg tacggagtgg aatcagcaac ggatgaagga cttgagttcc
     6781 aatacccagg cgctgaccgg ggcgaacacc ggcggcgcta ttgatggtgt gagtgccacc
     6841 gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caacatggag
     6901 ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc
     6961 aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctcaaa
     7021 atgggcattg cccgtgctta taaagcaccg agcctgtacc agactaaccc gaactacatt
     7081 ctctacagta aaggtcaggg ctgctatgcc agcgcgggcg gctgctatct gcaaggtaac
     7141 gatgacctga aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac
     7201 ggctggctgg cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc
     7261 tatgtggctg tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg
     7321 aaagcagtgg ttgaaggtct ggaaggatcg ttaaacgtac cagttagcga aacggtgatg
     7381 tggaccaata acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg
     7441 tcgatcatcc cggagtacac gttgaactca acgctgagct ggcaggcgcg ggaagatttg
     7501 tcgatgcaaa cgaccttcac ctggtacggt aagcagcagc cgaaaaaata caactacaaa
     7561 ggtcagccag cggttggccc ggaaaccaaa gaaatcagtc cttacagcat tgtgggtctg
     7621 agcgcgacct gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caacctgttc
     7681 gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc aggggccaac
     7741 tatatcgcgg gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtacatgagc
     7801 gtaaacactc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc
     7861 ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag
     7921 gaccatgtag gcctgataag cgtagcgcat caggcagttt agcgtttgtc atcagtctct
     7981 aatatggtcg acatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat
     8041 tttgttgagt tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcattac
     8101 gcgcaactgc aacacgctgg acggaagcgc aaagccgaac atttagccgg gcggatcgct
     8161 gcaatttatg cgctgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc
     8221 caaccggtgt ggcctgcgga ggtatacggc agtattagcc actgcggggc tacggcattg
     8281 gccgtggttt cccgtcaacc gattggcgtt gatatcgaag aaattttttc tgcgcaaaca
     8341 gcgaccgaat tgacagacaa cattattaca ccagcagaac acgagcgact cgcagactgc
     8401 ggtttagcct tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag
     8461 gcaagtgaga tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa
     8521 cagcaggtca tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa
     8581 aaaatagtca taactctgtg ccaacacgat taattgacaa catctggtac gattcgcccg
     8641 cagccatcac tgaccgcggg cgaaagtgta aagcaggtgc cttaccactc tgacctgaca
     8701 accggatatg cggggattgc tccccgcaca acggctactt cttcggttcg taagcgagaa
     8761 cagccttaaa ctcaatatta cgttccttta cggtgaactc acacagcgag tctccgacca
     8821 gaagcgtaaa tcccagtacc gtcaaacaca gcactatgac tgccacaagg gcatatttcg
     8881 tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt gttcaggttt
     8941 gggtaggtag ccccagggtt gatagaaata tcgtcctggg gcttcatctt tccagacctc
     9001 agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc ttgcttagca
     9061 aaatagctgt ttatttatac agcaagtcct gtttcggact tctcactccc catttgatca
     9121 actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt cgccaccagt
     9181 cagcactgcc ataacttgtc acattgcaaa agtagccaaa ttgcttctgt cataatcagt
     9241 tcaccatcac caaaaaaaag aaacattcca gaacttccgc atctcgccca gccgctccca
     9301 cttctatact gaatagaaaa cgccaacata agagaaacct atgccattac ccgattttca
     9361 tgtttctgaa ccttttacac tcggtattga actggaaatg caggtggtta atccgccggg
     9421 ctatgactta agccaggact cttcaatgct gattgacgcg gttaaaaata agatcacggc
     9481 cggagaggta aagcacgata tcaccgaaag tatgctggag ctggcgacgg atgtttgccg
     9541 tgatatcaac caggctgccg ggcaattttc agcgatgcag aaagtcgtat tgcaggcagc
     9601 cgcagaccat catctggaaa tttgcggcgg tggcacacac ccgtttcaga aatggcagcg
     9661 acaggaggta tgcgataacg agcgctatca acgaacgctg gaaaactttg gttatctcat
     9721 tcaacaggcg accgtttttg gtcagcatgt tcatgttggc tgcgccagtg gcgatgacgc
     9781 catttatttg ctgcacggcc tgtcacggtt tgtgccgcac ttcatcgccc tttccgccgc
     9841 gtcgccatat atgcaaggaa cggatacgcg ttttgcctcc tcacgaccga atattttttc
     9901 cgcctttccc gataatggcc cgatgccgtg ggtcagtaac tggcaacaat ttgaagccct
     9961 gtttcgctgt ttgagttaca ccacgatgat cgacagcatt aaagatctgc actgggatat
    10021 tcgccccagt cctcattttg gcacggtgga ggttcgggtg atggataccc cgttaaccct
    10081 tagccacgcg gtaaatatgg cgggattaat tcaggctacc gcccactggt tactgacaga
    10141 acgcccgttc aaacatcagg agaaagatta cctgctgtat aaattcaacc gtttccaagc
    10201 ctgtcgctat gggctggaag gcgtcattac cgatccgcac actggcgatc gtcgaccgct
    10261 aacggaagac accttgcgat tgctggaaaa aatcgcccct tccgcacata aaattggtgc
    10321 atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc ggtctgaatg aagcgcagct
    10381 gatgcgcgat ttcgtcgccg atggtggctc gctgattggg ctggtgaaaa agcattgtga
    10441 gatctgggcc ggtgactaag tagggaattg ccttacagaa gaatatccct gacaatggtg
    10501 ttttaactta catttaacaa tgttatgaaa caccctttag aaaccttgac taccgcagca
    10561 ggcattttgc tgatggcttt cctctcttgc ctgctgctgc ccgcccccgc actggggctg
    10621 acgctggcac aaaaactggt gaccatgttc catctgatgg atcttagcca gctttacact
    10681 ttattgtttt gtctgtggtt tttagtgctg ggcgctattg agtattttgt tctgcgcttt
    10741 atctggcgac gctggttctc gctggcggat taaacgtcga ttatcgccgc acttcgcggc
    10801 atactttgct tattctcctt cgcctaaagg aacgtttatg gataagcaat cactgcacga
    10861 aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag ctttgctggc cttttggccc
    10921 ggagttcgat gtttttaaaa ttggcggcaa aatttttatg ctgtcgtcgg agctgcgcgg
    10981 cgtccccttt atcaatctga agtccgatcc acaaaaatcc ctgttaaatc agcagatata
    11041 cccgagcatt aagccagggt atcacatgaa taaaaagcac tggatttcgg tgtatcccgg
    11101 cgaggaaatc tccgaagcat tacttcgcga tctgatcaac gattcgtgga atctggtggt
    11161 tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca ggctaaagcg gaaatctata
    11221 gcgcattttt ctcgcttacc atttctcgtt gaaccttgta atctgctggc acgcaaaatt
    11281 actttcacat ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc attccactaa
    11341 ggcatttgat gccagcaaaa aacttacccc ggaacaggcc gagcagatca aaactctcct
    11401 gcaatacagc ccatccagca ccaactccca gccgtggcat tttattgttg ccagcacgga
    11461 agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat tatgtgttca acgaacgtaa
    11521 aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg acgatgcctg
    11581 gctgaagctg gttgttgacc aggaagatgc tgatggccgc tttgccacgc cggaagcgaa
    11641 agccgcgaac gataaaggtc gcaagttctt cgctgatatg caccgtaaag atctgcatga
    11701 tgatgcagag tggatggcaa aacaggttta tctcaacgtc ggtaacttcc tgctcggcgt
    11761 ggcggcgctg ggtctggacg cggtacccat cgaaggtttt gacgccgcca tcctcgatgc
    11821 agaatttggt ctgaaagaga aaggctacac cagtctggtg gttgttccgg taggtcatca
    11881 cagcgttgaa gattttaacg ctacgctgcc gaaatcacgt ctgccgcaaa acatcacctt
    11941 aaccgaagtg taattctctc ttgccgggca tctgcccggc tatttcccct cagattctcc
    12001 tgatttgcat aaccctgttt cagccgtcat cataggctgc tgttgtataa aggagacgtt
    12061 atgcaggatt taatatccca ggttgaagat ttagcgggta ttgagatcga tcacaccacc
    12121 tcgatggtga tgattttcgg tattattttt ctgaccgccg tcgtggtgca tattattttg
    12181 cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg gctttggttg
    12241 caaatcatta cccagaataa actcttccac cgtttagctt ttaccctgca ggggattatc
    12301 gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga tattctgact
    12361 acctgcgcgc agttgtggat catgatgtat gcgctgcttt cagtcttctc gttgctggat
    12421 gtgattttga atctggcgca gaaattcccg gcggcatctc agttaccgct gaaagggata
    12481 tttcagggga ttaaactgat cggcgcgatt ctggtcggca ttttgatgat ctcgctgctg
    12541 attggtcagt cgccagcgat tctgatcagc ggtcttggtg caatggctgc cgtgctgatg
    12601 ttggttttta aagatccgat tcttggtctg gtggcaggta ttcagctttc cgcgaacgat
    12661 atgctgaaac tgggcgactg gctggagatg ccgaaatacg gcgcggatgg cgcggtgatc
    12721 gatattgggt taaccaccgt caaagtgcgt aactgggaca ataccattac cactattccc
    12781 acctggtctc tggtttctga ctcctttaaa aactggagcg ggatgtcagc atctggcggg
    12841 cgacgtatta agcgcagtat cagtattgat gtcaccagta ttcgttttct tgatgaagac
    12901 gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt atttaaccag ccgccatcag
    12961 gaaattaatg agtggaatcg ccagcaaggt tctacggagt cgatattaaa cctgcgccga
    13021 atgaccaata ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa ccatccgcgg
    13081 attcgtaaag atatgacttt aatggtacgc caactggctc caggtgataa cggtttaccg
    13141 ctcgagatct atgcgtttac caacaccgtg gtgtggctgg aatatgaaag cattcaggct
    13201 gatatattcg atcacatatt tgcgattgtc gaagagtttg gtctgcgact tcatcagtcg
    13261 ccaaccggca atgatattcg ctctctggcg ggtgcattta agcagtaatt aaaaaaaccg
    13321 ctctcatcga atggatgaga gcggacatcg ttttgtcgga tgtagcgtga atgccttatt
    13381 tccgacgcag cgttttaaat gccacaaaca ggaatacaat ccacaccggc agcaggatcg
    13441 ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatgccg aggaaggcaa
    13501 tgcaaagata gttgccgaac ggatagagca gcgccttaaa ctgtgtttca cgcccctgac
    13561 gtcgcatcgc tgcacgaaaa cgcagatgcg ccaggcagat cataatccag ttcaacagca
    13621 gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc agatagttga
    13681 ttaataccac cagcgaagtg atcgctccgg aaagcatcag cgaattaatc ggcacaccgc
    13741 gacggctgac gcgagtcaaa aacttcggcg cattaccctg cacagaaagg ccaaacagca
    13801 tgcggctgtt agagtaaacc ccgctgttat acactgacag cgatgctacc agaatgacga
    13861 agttcagcgc agaagctacc acgttgctgt cgagattatg gaaaatcatc acaaacgggc
    13921 tactgttgga tttcacttcc acccacggat agagcgccag taaaaccacc agtgagccga
    13981 tgtaaaacag caggatgcga tacaccacct gatttaccgc ttttggaatg cttttttccg
    14041 gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag accgccgaag gagaacataa
    14101 ttaccgccag cgacaaaatc agcccattcc agccggtggc aaagaaacca ccgtagcgcc
    14161 agaggttgtc gatactggct ttctcgccgc cgtgaccaga aaacagcagc cacaggccaa
    14221 agccgatcat accgatgatc gccagcactt taatcagcgc aaaccagaac tcggtttcac
    14281 catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag gcggcagccc
    14341 aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca gcggtcagct
    14401 ctgccattcc caccagcacg aacattaccc agtagttcca gccagagagg aagcccgcaa
    14461 acggtcccca gtatttatag gcaaagtggg caaatgaacc ggataccggc tcttcgacca
    14521 ccatctcgcc aagctggcgc ataatcagga aagcgatgat cccggcgacg ccgtagccca
    14581 gcaatacagc cggacccgcc atctgaatcg ccggaccaat gccaagaaac agaccagtac
    14641 caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg cgatgaagcg
    14701 tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc acgcctttcc
    14761 cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc gtcgcaagac
    14821 acaatccaca cggttaaacg gggtatcctg cttttatttc cgtacccgat gtcggtgcag
    14881 ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggtg cggtgatcat
    14941 gccgccaatc atcggtgcgg caatccggct catcacctct gaaccagccc ccgttcccca
    15001 cagaatcggc agcagaccgg cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg
    15061 cagtaccgcg ccgtgataca acgcctcatc cagcttctgc tcactgaatg tttgcggatt
    15121 attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca ccacgccaaa
    15181 ttcggcggcg accccggcaa gggcaataaa accggtgccc gttgccacag aaagatgaaa
    15241 gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc tgctgataat
    15301 cagcaacgct tcgcccaccc gacggaatgc cagatataac agcacgaaga tgatcatcag
    15361 tgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct cgaactgccc
    15421 ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg ctttttgcaa
    15481 atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg
    15541 gcgcgcattc tcggttttca gcatcgacgg tccggtagag actttaacgt cggccacgtc
    15601 tgccagggtg atttgctgct tcatcggcgt caggatcggc aactggcgca gtgcctgcgg
    15661 actatcgcgc cagctttgcg gataacgcag attaatggga taacgggcaa tcccttccac
    15721 cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca catccgccac
    15781 cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat agcgtccacc
    15841 ttccagacgc tcggcaagcg cagaagccac gcctggtacc gttcgtgcca cctcttcaat
    15901 ttgctccgcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa tgccgatggg
    15961 gcttttaatg ccagtcgaga gcatatcgat acggttacga attggcggca cccacagatt
    16021 cgccagcccc ggcagacgca cggtgttatc cagttcctca atgattttgt ccatcgtcat
    16081 gcctggccgc cactgctcct gcggcttaag ctggatggtc gtttctacca tctccagcgg
    16141 agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg ccacttcagg
    16201 tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg ctgcggaaat
    16261 ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc
    16321 cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgccg ccaccagcag
    16381 cgtggttttc ggccagtgca gtactttcag caacagcgga tgataaacac gaatcaaaaa
    16441 gcgattgagc gggttactgc tttccggcgg aattttgcca cggatccagt agcccatcag
    16501 gatcgggatc actacgatcg ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa
    16561 cgccaacggg ccaaacagac gcccttcctg cccttccagg gtgaagatcg ggataaacga
    16621 caacgtgata atcagcagac tgataaatag cgccggcccc acttcaacag acgcatcggt
    16681 gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt gctgccactc
    16741 ttccagccgt ttatgcgcat tctcgatcat gacgatagca gcatcgacca tcgccccgac
    16801 ggcaatcgca atgccaccca acgacataat attggcattc agtccctgga agtgcatgac
    16861 aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac
    16921 atgccagaga aacagcgcac agactaccgc caccacaata aactcttcca gcaacttgcc
    16981 gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga tcgtatgttg taactatctc
    17041 cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg aggcgatcac
    17101 ttctcgggcg tttttgccgg agcgcaggat caccaccccg cccgccactt caccttcgcc
    17161 attcagttcg gcaatgcccc ggcgcatctc cgggccaatc tggaccttcg caacatcacg
    17221 cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa agtcgtcgaa
    17281 cgtttgcaga tagccgctgg cgcgcaccat atattccgct tccgccagtt cgatcgacga
    17341 accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcta gactgatgcc
    17401 atattgcgcc aggcgctggg gatcgataac cacctgatac tctttcacca cgccgcccac
    17461 cgacgccact tccgcaacgt cagggatggt ttttagctca tatttgagaa accagtcctg
    17521 caatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg catattcata
    17581 gatccagcca acacccgtgg catctggtcc cagctcggcg ctgactcccg caggcagctt
    17641 accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg gatcggtacc
    17701 atcttcgaaa atgacataca cataagagtc tccaaactgc gagaaaccgc gcacagtctt
    17761 cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat tttcaacgat
    17821 ttgcggtgcc tgaccgggat agctggtttt aataatcacc tgcacatcgg agagatccgg
    17881 cagcgcatcc actggcgtgt taatgatggt ccaggtgccc cagatgctca gaaacaacgc
    17941 gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt caatcattgg
    18001 ttattccctc aatgcgcatt ggtagcactt tcagagcgca tccgctccag tgcgccagaa
    18061 atattggctt cggaatcaat caggaacagg ccgctggaaa ccaccttttc accttccgcc
    18121 agaccagagt gtaacgcggt gacgccttgc gatgcctgga aaacggcaac gcgtttcggt
    18181 acaaagcgtc cgtcggcatc aacggtaatc acccgctgtt cgttgccggt atcaatcagc
    18241 gcctgtgacg gaatgagcag catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc
    18301 atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg
    18361 gtcgcggcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt tttatccggt
    18421 cgcgccggaa cggtgagcgt aaactgcgag gcatctttca ccagccaggc gatagactcc
    18481 gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgctac cacgttatct
    18541 ttggcgatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc aatgggtgct
    18601 ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat atccgcctcc
    18661 ggcattcccg ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc cgtaccgccg
    18721 gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcaggaat ggtcaggtcg
    18781 agaagcggtg tgcccttttg tactttatcg cccacggtaa gcggatacac cttgtcgata
    18841 aacccggcag cgcgagcctg cacaatggca tactgatact cgttgtaact gacattcgcc
    18901 gggaaactct gggcaaaagt cagcggcccg cgcgtgacgg tagccgtttt cacccccaga
    18961 ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc atcggcatat
    19021 ttcggcacca gagccatatc cataaacggc gatttacctg gtttatcgaa ccgcgtattg
    19081 ggatacatcg ggtcgtacca gaataagatt ttacgttctg cggtcgacgt tttttctgcg
    19141 ggtggttccg cctttgcaaa ccaggtaaaa cctgccgcag aaataatacc gcccgcgatc
    19201 atgctgccga taataagcgc gatttttttc atctcattaa acctgggtta ctggctgact
    19261 ttaatatcct gtaataaaga aaggttgccc tgctggacaa aattaaacgc cactttgtcg
    19321 ccagttttaa tttcactcat tttcgtctgc ggagtgatgg taaagcgcat ggtcatctcc
    19381 ggccagttca cggcagcaat cggatcgtga tggatggtga tttttttgct ttccagatca
    19441 atacccttta cctcgccagt ggcgctaata acctgtggtt gtgcttcgct catggtttca
    19501 tgatgatgtt cgttagcctg ggcattaaag ccaataacgg ttaacagact gaacattgcg
    19561 acttgcagtg cttttttcat ttattctctc ctggagttaa aagttattgc tgccaaccgc
    19621 caccaagtgc ggtatataaa gaaatttcgt taacctgacg ggcataattc agatcaagta
    19681 aagtttgtcg ggttgcaaat aaagaacgct cggcatccag cacttccaga taacttactg
    19741 cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgac gccagataac
    19801 gttgctgggc gctaatttga tcgttcaggc tttgacgtaa tgccagcgca tctgccactt
    19861 ctttaaaggc gttctggatt ttctgttcat aattcaccac cgactgctgc tggcgaattt
    19921 cggcgatatc cagattggcc tggttgcgtc cggcattaaa aatggggatc tcaattttag
    19981 gaataaaatt ccacatcccg ctgctagcgt taaataatga agacagatcg ctgctggcgg
    20041 ttgatattcc gctggtcagg ctgatagacg ggaaaaatgc cgcacgcgca gcgccaatat
    20101 tggcattagc cgccattaac gcgtgttcag cttccataat atccgggcgc tgcaataaga
    20161 tttgcgacga caatcccgcc ggtaatttaa cgctttgcag gctgtcgctg tttactgtct
    20221 gcgcttgcgg cagcttgccg tagcttccca ataacagttg caatgcatta ttcgcctgcg
    20281 ccagttcccc ctgacgttta gcgatgtcgc tgcgggtact ttcaatcacc ccgcgagcct
    20341 gttccagcgc cagaacatta ctgctacctg tcaacagttg tttttcgaca aacgcatatg
    20401 actgctgata attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac gctagctgct
    20461 gattgaaata actttgcgcg acattagaaa ccagcaaaat atgcaccgcg cgctgggctt
    20521 cctcagtggc taaataattt tggcgctcgg cttcgctcat gttctttaag cgaccgaaaa
    20581 aatcgagatc aaagctggcg ttaaggccgg tcgaaaactc ccgtgtcgtg gcggtattcc
    20641 ctttaagatt accgctccag ctgccgctgc cctcgccatt gagctgtggg tagcggtcgg
    20701 catcggtcag acgatattgc gcccgcgctt cctgcacttt cagcgtcgcc atgcgcaaat
    20761 cccggttatt caccagcgcc tcgctaatca gcgtctttac ctgattatca acaaaaaagg
    20821 tgcgccagcc cacgttctgg tagttatctg ctgcgttaac caagccgttc tggctgagtg
    20881 agaactgctg cggcacgggc attgccggac gctgataatc cggtgccagt gaacaaccgg
    20941 ttagcgcaag ggccacacaa aatggcagaa gtttacaagg agacataggc tcataatttc
    21001 tggtgatttt ataccgccaa ctttactcgc caggctctga ttttccggtg acaggaaaat
    21061 gacaaaattg tcattttgcc actaagcgat tgccatctga tcccgctact ctagaattgc
    21121 ccgggcaaca tgcggaggaa aaatgaaact gttgattgtc gaagatgaaa agaaaaccgg
    21181 agaatacttg accaaagggt taaccgaagc cggttttgtg gtcgatttgg ccgacaacgg
    21241 gttgaatggc taccatctgg cgatgaccgg tgattatgat ctgataatcc tcgatattat
    21301 gctgccggac gtgaacggct gggatatcgt gcgcatgttg cgttccgcca ataaagggat
    21361 gccgattctg ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg ggctggagtt
    21421 gggggccgat gactatctgg tgaagccgtt cgcttttgct gaactgctgg cgcgggtgcg
    21481 cactctcctg cggcgcgggg cggcggtgat tatcgaaagt cagtttcagg ttgccgatct
    21541 gatggtcgat ctcgtcagcc gcaaagtcac ccgcagcggc acgcgcatca ctctgaccag
    21601 taaagagttt actctgctgg agttttttct ccgccatcag ggcgaagtgc tgccccgctc
    21661 gcttatcgcc tcgcaggtgt gggacatgaa tttcgacagc gacactaacg ccattgatgt
    21721 agcggtgaag cggctgcgcg gcaaaatcga caacgacttt gagccgaagc taattcagac
    21781 cgtgcgcggc gtgggctaca tgcttgaggt gccggatggt cagtaagcca tttcagcgcc
    21841 cgttttccct ggcaacccgc ctgacctttt ttatcagcct ggccaccatc gcggcgtttt
    21901 tcgcctttgc atggatcatg atccactcag taaaagtgca ttttgccgag caggatatta
    21961 atgatttaaa agagattagc gccacgcttg aacgggtact taatcaccct gacgaaacgc
    22021 aagcccgacg cttaatgacg ctggaagata tcgtcagtgg ttattccaac gtgttgattt
    22081 ccctggcaga tagccacggc aaaacggtgt atcactcccc cggtgcgcct gatatccgcg
    22141 agtttgcacg tgatgccata cccgataaag acgcgcgggg tggcgaggtg tatctccttt
    22201 ccggcccgac gattatgatg ccaggccacg gtcacgggca tatggaacac agcaactggc
    22261 ggatgattaa cttgtcggtt ggcccgttgg tggacggcaa accgatttat acgctctaca
    22321 tcgcgctttc gatcgatttt catcttcatt acataaatga tttgatgaat aaacttatta
    22381 tgaccgcatc ggtaatcagc atcctgatcg tctttatcgt actgttggcg gtacataaag
    22441 gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa tatcacctcg aaagatctcg
    22501 acgttcgcct tgacccgcag accgtgccca ttgagctgga acagctggta ctgtcgttca
    22561 accatatgat cgagcgtatt gaggatgtct ttacccgcca gtccaatttc tcagcggata
    22621 tcgctcacga aattcgcacg ccgattacca atctcatcac gcaaacggaa atcgccctca
    22681 gccagtctcg cagccagaag gagctggaag atgtgctcta ctctaatctc gaagagctga
    22741 cgcgaatggc gaaaatggtc agcgatatgc tgtttctcgc tcaggccgat aacaaccagc
    22801 taatccccga aaagaaaatg ctcaacctgg cggatgaagt cggcaaagtg ttcgattttt
    22861 tcgaggcgtt agcggaagat cgcggcgtgg agttgcgatt tgttggcgac gaatgtcagg
    22921 ttgcgggcga tccgctgatg ctgcgtcggg cgttaagcaa cctgctttct aatgccctgc
    22981 gttatacgcc gcccggagag gcaattgtag tgcgctgcca gacggtcgat caccaggtgc
    23041 aagttaccgt cgaaaacccc ggtacgccca ttccgcccga gcacttaccg cgattgtttg
    23101 accgtttcta ccgcgttgac ccttcccgcc agcgaaaagg cgaaggtagc ggcattgggc
    23161 tggcgatagt gaaatcgatt gttgtcgcgc ataaaggcac ggttgcagta acgtcagatg
    23221 cgcgggggac aaggtttgtg attatgctgc cggagagaga gtgattatta agttcaaatt
    23281 cagggcgtgg tg
//
LOCUS       DAZFAH010000056        23249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFAH010000056 DAZFAH010000000
VERSION     DAZFAH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.056"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..1036
                     /gene="pspF"
                     /locus_tag="ACHJWB_002092"
     CDS             59..1036
                     /gene="pspF"
                     /locus_tag="ACHJWB_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4313730.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(1156..2421)
                     /gene="puuE"
                     /locus_tag="ACHJWB_002093"
     CDS             complement(1156..2421)
                     /gene="puuE"
                     /locus_tag="ACHJWB_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4313731.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(2459..3739)
                     /gene="puuB"
                     /locus_tag="ACHJWB_002094"
     CDS             complement(2459..3739)
                     /gene="puuB"
                     /locus_tag="ACHJWB_002094"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4313732.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSMRDRLGV"
     gene            complement(3741..5228)
                     /gene="puuC"
                     /locus_tag="ACHJWB_002095"
     CDS             complement(3741..5228)
                     /gene="puuC"
                     /locus_tag="ACHJWB_002095"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4313733.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(5503..6060)
                     /gene="puuR"
                     /locus_tag="ACHJWB_002096"
     CDS             complement(5503..6060)
                     /gene="puuR"
                     /locus_tag="ACHJWB_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4313734.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(6087..6851)
                     /gene="puuD"
                     /locus_tag="ACHJWB_002097"
     CDS             complement(6087..6851)
                     /gene="puuD"
                     /locus_tag="ACHJWB_002097"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4313735.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            7063..8481
                     /gene="puuA"
                     /locus_tag="ACHJWB_002098"
     CDS             7063..8481
                     /gene="puuA"
                     /locus_tag="ACHJWB_002098"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4313736.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            8630..8794
                     /locus_tag="ACHJWB_002099"
     CDS             8630..8794
                     /locus_tag="ACHJWB_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313737.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRRGSNYGY"
     gene            8784..10169
                     /gene="puuP"
                     /locus_tag="ACHJWB_002100"
     CDS             8784..10169
                     /gene="puuP"
                     /locus_tag="ACHJWB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4313738.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            10303..10548
                     /gene="ymjA"
                     /locus_tag="ACHJWB_002101"
     CDS             10303..10548
                     /gene="ymjA"
                     /locus_tag="ACHJWB_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO4313739.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            10861..12504
                     /gene="sapA"
                     /locus_tag="ACHJWB_002102"
     CDS             10861..12504
                     /gene="sapA"
                     /locus_tag="ACHJWB_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4313740.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCVPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            12501..13466
                     /gene="sapB"
                     /locus_tag="ACHJWB_002103"
     CDS             12501..13466
                     /gene="sapB"
                     /locus_tag="ACHJWB_002103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4313741.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            13453..14343
                     /gene="sapC"
                     /locus_tag="ACHJWB_002104"
     CDS             13453..14343
                     /gene="sapC"
                     /locus_tag="ACHJWB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4313742.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            14343..15335
                     /gene="sapD"
                     /locus_tag="ACHJWB_002105"
     CDS             14343..15335
                     /gene="sapD"
                     /locus_tag="ACHJWB_002105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4313743.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            15337..16143
                     /gene="sapF"
                     /locus_tag="ACHJWB_002106"
     CDS             15337..16143
                     /gene="sapF"
                     /locus_tag="ACHJWB_002106"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4313744.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            16211..16564
                     /gene="ycjD"
                     /locus_tag="ACHJWB_002107"
     CDS             16211..16564
                     /gene="ycjD"
                     /locus_tag="ACHJWB_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO4313745.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            16932..17720
                     /gene="fabI"
                     /locus_tag="ACHJWB_002108"
     CDS             16932..17720
                     /gene="fabI"
                     /locus_tag="ACHJWB_002108"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4313746.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            17865..18992
                     /gene="yciW"
                     /locus_tag="ACHJWB_002109"
     CDS             17865..18992
                     /gene="yciW"
                     /locus_tag="ACHJWB_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO4313747.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTSWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            19060..20994
                     /gene="rnb"
                     /locus_tag="ACHJWB_002110"
     CDS             19060..20994
                     /gene="rnb"
                     /locus_tag="ACHJWB_002110"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4313748.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(21072..21176)
                     /locus_tag="ACHJWB_002111"
     CDS             complement(21072..21176)
                     /locus_tag="ACHJWB_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313749.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            21230..23215
                     /gene="pdeR"
                     /locus_tag="ACHJWB_002112"
     CDS             21230..23215
                     /gene="pdeR"
                     /locus_tag="ACHJWB_002112"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4313750.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGNQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSATIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         5254 a         6099 c         6142 g         5754 t
ORIGIN      
        1 caacagcaac atgccaggat gagttagcga attacactaa caagtggcga atttcatcat
       61 ggcagaatac aaagataatt tacttggtga ggcgaacagc tttctcgaag tgctggaaca
      121 ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg
      181 taaagagctg attgccagcc gcctgcatta tctctcctcc cgttggcaag ggccgtttat
      241 ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt ttggtcacga
      301 agcgggggcg tttaccggtg cgcaaaaacg tcatccaggg agatttgaac gtgccgacgg
      361 cggtacgcta tttcttgatg aactcgctac ggcaccgatg atggtgcagg agaaattatt
      421 gcgcgtgatt gagtacggtg aactggagcg cgttggcggc agccagccat tgcaggtgaa
      481 tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg atggtcaatg aaggcacttt
      541 tcgcgctgac ctgctcgacc gactggcttt tgatgttgta caactgccac cactgcgcga
      601 gcgcgaaagc gacataatgt tgatggcaga acactttgcc atccagatgt gtcgggaaat
      661 caagctgcct ctgttcccgg ggtttacgga gcgcgccaga gaaacattgc tgaattatcg
      721 ttggccggga aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg
      781 caccagcgat tatccgcttg atgacatcat tattgatccc tttaaacggc gtccgcctga
      841 agacgctatc gccgtttcag aaaccacctc gcttccaaca ctgccgctgg atttacgtga
      901 gtttcagatg cagcaggaaa aagagttgct gcaactcagt ttgcaacaag ggaaatataa
      961 ccagaagcgc gcggctgaat tactggggtt aacttatcat cagttccgcg cgttgttgaa
     1021 aaagcaccag atttagcgaa gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt
     1081 attcggcata cataatcaat acgtcgtttg caggcctgat aagcgcagcg catcagacat
     1141 tattgcgttg ggctattaat cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa
     1201 ttgcgcatcc gggatggtca gcggatacag gaagcgaatc acgttgccgt atgcgccaca
     1261 ggtcagcagg agcagcccct gcgccagcgc gcgttgctgg attttctgtg caatcgccgc
     1321 tgacggctcg cccgtttgcg gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg
     1381 taccgcagca atggccggaa cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg
     1441 gcccagttga ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga gcaccgcgtg
     1501 cgcggcagcc accgccagcg ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg
     1561 cgcgtccata atattcgcgt taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct
     1621 tttcgccatc gtcattaaat ccggcttatc gacgtaatga tccatggcaa acagtttacc
     1681 ggtacgcgca aagccgcttt gcacttcatc ggcaatcatc acaatgccgt gctcgtcgca
     1741 caggcggcga atggcggcaa ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg
     1801 caccggttcg aaaataatcg ccgccacctg cttcgcttcg atgtctgatt taaacaagcg
     1861 ttcgatggcg tcgagggagt cctgtgttga aatgccgtgt aaatctgacg gataaggtac
     1921 gtgatacacc gaaccaggga acgggccgaa gccgattttg tacggcgcga cctttccagt
     1981 taacgccata gtcatatacg tacgaccgtg gaagccgccg ctaaacgcaa tcacgccagg
     2041 gcgtccggta tgggcgcgag caattttcac cgcgttttcc accgcttccg caccggtagt
     2101 gaagaacgcg gttttggcct gcccgctcac cggggcaagg gcgttgattt tctccgccag
     2161 ggtgacgtag ctttcatacg gcacaatctg atacgcggtg tgggtaaact gttgcagttg
     2221 ctgctccacc gccgcgacca actccgggtg gcgatgcccg gtattcagca ccgcaatgcc
     2281 tgcggcgaaa tcgatgtatt cgttgccctc aacatccttc agcgtggcgt tttcagccga
     2341 ctgggcgaag aagttacaca tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg
     2401 atggaattca ttgttgctca tgtttcacac cttttgactc ttcactgtta atcggaaatt
     2461 aaacgcccag acgatcgcgc atgctgtaat aggctgcgcc catcgcggta aacggtactc
     2521 gcagcgtgcg cccgccgggg aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt
     2581 cggcgtcgcc gcgcagcagt tcggcaatca gacgcccggc aagatgggta caggtgacgc
     2641 cgtggccgct gtagccctgc atgtaataga tgttggtatc gaggcgaccg aactgcggca
     2701 tccgtgaaag ggtcagcagg aagttgcccg tccagcggta gtcgattttg acgcccttca
     2761 gttgcgggaa ggttttcagc agtttcggca ccacaagacg ctcaacgtca tccgggtcgc
     2821 gcgcgccgta gaccacgccg ccgccgtaca gcaaacggtt gtcggcggta agacggtagt
     2881 aatcaagcag ataattacag tcttccacac agtagttttt cgggatcagc gaacgggcta
     2941 aatcttccga cagccgttcg gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg
     3001 ccagttccgg ctccacttta tcgcccaggt acgcattccc ggcgacaatc acatacttcg
     3061 ccgtcacctg acctttggca gtacgcacca cggcgggcgt ggtgtgctgg atctgcgtga
     3121 ccgcagaaag ctcatacacg cgcccgccgt tcaggcggat ggcatcggct tcaccaatgg
     3181 cgaggtttag cgggtgaata tgcccaccgc tgtgatccag cagcgcaccc gtgtagcgat
     3241 cgctggctac ttcacggcga atggcgttag cgtccagcag ctccagctgt ttattgccgt
     3301 agcgttccca gttctctttc tgctcttcga gtgtggcgag ctgtttatca ttcatcgcca
     3361 caaacaggcc gccggggcgg tagtcgcagt caatctgata acgtttgata cgttcgcgga
     3421 tgatctcgcc accctcgaac atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc
     3481 ttttttcgat cacgtcgatg tcgcggctgt aggagttcac aagctgcccg ccattgcgcc
     3541 cgcttgcgcc aaagccgatg cgtgaggctt cgagaaccac tacgtcaaag cccgcttccg
     3601 ccagatgcag cgcggaggag agcccggtat agccgccgcc aaccacgcaa acgtcgcagg
     3661 tgattgactc attcagtgtg tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac
     3721 tactggtatg ttcggtcatt tcaggcctcc aggcttatcc agatggtttt cagttcagtg
     3781 aatttttcaa gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt atagccgcca
     3841 aacggcacgg tcatatcgcc gtcgttgtag ttattgacga agacggaacc ggctttcagg
     3901 cggcggctca tacggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg
     3961 tactggctgt cgttggcaag ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc
     4021 agcaccggac cgaaaatctc ttcgcggctt aaggacgcat tcgggtccac atccacaaag
     4081 atggtcgggc cgatggcggc agccagctcg gcattacggc catccagcag cagttgccct
     4141 ttgctttcgc cttcccgaat aaagctatgg accgagtcgg cgtgggcgca gtcgattaag
     4201 gtgcccatgg tggttgcggg atcaagtgga tggcccggct gccagttttg cgcctgctgt
     4261 tttaacaggg ctaagaattc atcggcgatg ctctcttcca gtaacaaacg cgttccggcg
     4321 atgcacacct gaccctggtt gtagaagatg cctgctgcgg tggagctcgc cgctttttgc
     4381 agatccggac aatcggcgaa aacgatgttg gcgcttttgc cgcccgcttc cagccagacg
     4441 cgtttcatgt tgctgtcgcc cgcatctttc agcagctgtt tcccggtacg ggttgaaccg
     4501 gtaaaggcaa tggcgtcgat atcgttatga cgcgacagcg cctgcccggc ttcatgacca
     4561 aaacccgtca ccacgtttaa cacaccatcc ggcaagcctg cttctttcgc cagccccgcg
     4621 agacgaatcg cactgagcgg tgatttttca gacggtttta gaatcacgct gtttcccgcc
     4681 gccagcgccg ggccgagttt ccagcaagtc agcaacagcg ggaagttcca cggcacgatg
     4741 gcggcaatca cgccgaccgg ttcacgcacg atcatcgcca gctcatggct actggtggtc
     4801 gccacttcgc catacacttt gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg
     4861 ccgggaatat catcacgcag actgtgacga atcggtttgc cggtgtcgag agtttccagc
     4921 agtgccagct cttcggcgtg ggcttccatt aaatcggcga gtttattcag taccgcttta
     4981 cgtttagccg gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc
     5041 atcgcacggt cgatatcgac gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg
     5101 accggatcaa cggtttcaaa ggtttcattt tccgccgcag cagtatattc accgttaata
     5161 aataagcggt tttcaatggc gagacttaac gctttatcct gccagtaagc cagatgatga
     5221 aaattcatta tgactcctgt ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg
     5281 cgcagagatc cccgggccgt atgcccgttt atttctggcg acattcttta tatacacttc
     5341 atccttcaag ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt acctgagtaa
     5401 gctcatcggg attcgctcac ttgccgcctt gatgcaactt gaatgatttt gtgtatagat
     5461 tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt
     5521 atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc
     5581 ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag
     5641 aacaatttca ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc
     5701 agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc
     5761 gttatgaacc agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt
     5821 aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag
     5881 accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc
     5941 tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag
     6001 cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat
     6061 atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga
     6121 caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat
     6181 tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact
     6241 aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgc
     6301 ccatgcagag agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc
     6361 ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga
     6421 tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg
     6481 gttgccacca ccagttcctg taaaccccgg cagatggcga aaatggggat gcgcctttcg
     6541 agtgcggcat taattaacgc catgctcaga agatcacgcc cgggatcggc gtcaggctca
     6601 tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg
     6661 ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc atgtggtagc
     6721 gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg
     6781 gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt
     6841 atattttcca tgctcaatct cacaaagtgg actaaattat cgccattact gcggtttaaa
     6901 tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa
     6961 tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta
     7021 cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt
     7081 gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa
     7141 gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat
     7201 ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa
     7261 gggtgttatt tcccggcctc ggtgtttgca atggatattc tgggcaacgt agtagaagaa
     7321 gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt tctcggttcc
     7381 ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat
     7441 gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag
     7501 ctgcgccagc gcggattgtt cccggtcgta gcggtagagc tggagttcta tttactggat
     7561 cgccagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc
     7621 aatacacaaa gccaagttta ctccgttgat aacctcaacc acttcgccga cgtgctcaat
     7681 gatattgatg agctggcgca gttacagtta attcccgccg acggtgcggt cgccgaagcc
     7741 tcgccgggtc agtttgaaat caacctttac cataccgata acgtgctgga agcctgcgat
     7801 gatgcgctgg cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc
     7861 acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat ccatatcagt
     7921 atgcaaaata atcgtggcga gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg
     7981 ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc gttgctggca
     8041 ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg
     8101 tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg tcataatcac
     8161 cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt
     8221 tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc
     8281 aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag
     8341 tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac
     8401 gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag
     8461 tggatgttga aaaacgcgta attccccatg cgtggttgtt tcatcggcct gagcggagcc
     8521 aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc
     8581 ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat
     8641 taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg actggactcc
     8701 gccccctttg cttcccttgc taaacaggtt tgatttcagg ttgacacgac cgcaaacctt
     8761 attacgcagg agaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac
     8821 ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg
     8881 cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg
     8941 gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct
     9001 acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt
     9061 cgattaatcc gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt
     9121 tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc
     9181 cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg
     9241 tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg
     9301 tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt
     9361 cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga
     9421 ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg
     9481 acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct
     9541 ttatcgcggc gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc
     9601 cggacgccgc actgcctgaa attgcgctgt acgtcggcgg caagctcttt cagtcgattt
     9661 tcctctgcac cacgtttgtg aacacattag cgtctggcct ggcttcacat gccagcgtgt
     9721 cgcgtctgct gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg
     9781 tgcacccaaa atggcggact ccggcactca acgtcattat ggtcgggatt gtcgcgctgt
     9841 cggcgctgtt ctttgattta gtcaccgcga cagcactgat taacttcggt gctctggtgg
     9901 cgtttacctt cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa ggaatgaata
     9961 aaagctggaa ggatcacttc cactatttgc tgatgccgct ggttggcgcg ctgacggtgg
    10021 gtgtgctgtg ggttaaccta gagtcaacgt cactgacact cggtctggta tgggcttcgt
    10081 taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag
    10141 gtgacagaac gccggtgagc gaaacgtaat agccggatgc gcggcgcatt atcctgcctg
    10201 agggtaacgc gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat
    10261 catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg
    10321 ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag
    10381 gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat
    10441 gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
    10501 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc
    10561 agacgatcgg tcagtatcgt tgtccggata aagcgttcac gccgcatccg gctaacaatg
    10621 acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
    10681 agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg
    10741 acgccacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt
    10801 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt
    10861 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
    10921 gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc
    10981 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
    11041 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg
    11101 ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg
    11161 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
    11221 gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc
    11281 aacggcagca acttccccta cttcgacagt ctgcaatttg ctgataacgt gaaaagcgtc
    11341 cgtaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg
    11401 tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag
    11461 aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcaattg
    11521 tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt
    11581 aaaccgttaa tgccgcaggt ggtggtggat ttaggctccg gcggcaccgg acgtctgtcg
    11641 aaactcctga ctggtgaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt
    11701 ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcccg ggatgaacgt cgcctatctg
    11761 gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg
    11821 gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc
    11881 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacgat
    11941 ccggcgaaat cgcgcgaaca gttgaagtcg ttggggctgg aaaatttaac gctgaaactg
    12001 tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt
    12061 caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt
    12121 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac
    12181 agtaacgacc cggacagttt cttccgtcct ttactgagct gcgcggcaat tcattcacag
    12241 accaacctcg cccactggtg cgttccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
    12301 tcgcagcagc tggcagcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag
    12361 gaattgccca ttttgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
    12421 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
    12481 caggatgagg tgaaaaagcc atgattatct tcaccttacg ccgcattttg ttattgattg
    12541 ttaccttgtt cctgctgacc ttcgttggct tcagtttgag ctatttcact ccacacgcgc
    12601 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact
    12661 gggattttgg tgtttccagc attaacggtc aaccaattgc cgaacagtta aaagaggtct
    12721 tcccggcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
    12781 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
    12841 tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt
    12901 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga
    12961 aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg
    13021 aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc
    13081 caacaactga agtgatccgc ctgatgcgta tcagcactat cgaagtgtat gaccagaact
    13141 atgtaaaagc ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
    13201 tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga
    13261 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
    13321 acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
    13381 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
    13441 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
    13501 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
    13561 gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag
    13621 caatttctcg gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc
    13681 ttcctgggga ctgacgacct cggacgcgat gttttaagcc gcttactgag cggagctgcg
    13741 ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg
    13801 ggcaccttcg ccggggcgac acacggcctc cgctcagcgg tgcttaacca tattctggat
    13861 accttactgg cgatcccttc gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg
    13921 agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg
    13981 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
    14041 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg
    14101 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
    14161 ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg
    14221 gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc
    14281 gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa
    14341 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
    14401 ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
    14461 gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata
    14521 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
    14581 cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt
    14641 cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
    14701 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
    14761 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
    14821 ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
    14881 tgattgctga cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
    14941 tgctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
    15001 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa
    15061 ccgcgccgag taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc
    15121 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg
    15181 gcgctatccc actgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt
    15241 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
    15301 cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg
    15361 cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa
    15421 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag
    15481 aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga gcctaccagc
    15541 ggcgagttat tgattgacga tcatccactg cattttggcg attattcctt ccgcagtcag
    15601 cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctcg
    15661 caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa
    15721 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
    15781 catatgttgg cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc
    15841 ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcgat gcgttcgcag
    15901 ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc
    15961 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
    16021 gttgtcgagc gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa
    16081 cggctgattg ccgggcactt tggcgaagca ttgacggcgg atgcgtggcg taaagatcgc
    16141 taattttctg aatcctcctt ccagattatc cacctcattc tgacaagaga ttaaatcttg
    16201 tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagac gcaatctcac
    16261 tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt
    16321 tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgcgt
    16381 agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgata ccaggcgcac
    16441 tagctggctt gagtcgcagg gctggaccgt gctgcgtttc tggcataacg agattgattg
    16501 taatgaggag gcggtgctgg agattattct gcaggaactg aaccgccgat caccctcacc
    16561 ctgaaagggc gagggggccg accgagccga atagctgttg tggtgaaaac atggagacag
    16621 tgctggagaa tattcggcaa ggtctgaacc atcccagcca tcgccataaa agggttaggg
    16681 gctgtatgag cctgtttgtt gctggggtaa caatatttgc acaatatggt cccctcgccc
    16741 ctctggggag agggttaggg taaggggaaa agcgcccacc ctgccgcagc ctgctccggt
    16801 cggacctggc aactatagct actcacagcc aggttgatta taataaccgt ttatctgttc
    16861 gtactgttta ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa
    16921 ggattaaagc tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca
    16981 aactatccat cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat
    17041 tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt
    17101 ctgacatcgt tctgcagtgc gatgttgcag aagatgccag catcgacacc atgttcgctg
    17161 aactggggaa agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg
    17221 gcgatcagct ggatggtgac tatgttaacg ccgttacccg tgaaggcttc aaaattgccc
    17281 acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc
    17341 cgggttctgc cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca
    17401 acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga
    17461 tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg
    17521 cctccggtat caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc
    17581 gccgtaccgt tactattgaa gatgtgggta actccgcggc attcctgtgc tccgatctct
    17641 ctgccggtat ctccggtgaa gtggtccacg ttgacggcgg tttcagcatc gctgcaatga
    17701 acgaactcga attgaaataa tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg
    17761 ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg
    17821 ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca
    17881 tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactgcggag tatgacgttc
    17941 tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac gtgatccttg
    18001 atgaagaaac gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg
    18061 aattgttccc tgaccagctt accgtgaccc gttcacagac cttcaccgct tatgaacgct
    18121 tgtctacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt aactactatt
    18181 cggcgcgact tacgccgctc cccgggcctg attcctccag ggaaagcaat cataggttgg
    18241 cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat
    18301 cgcgccagca tctgaatgac gtcggtctta cttcctggga ctgtgtgatc attaaccaaa
    18361 tcattggttt tattggcttt caggcgcgga ccattgcgac atttcaggct tatctcgggc
    18421 acccggtacg ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg
    18481 ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct gaagagtaca
    18541 caaaaagttc aactgcagaa ctttgccaac tggccgaaac actctctctc caccctattt
    18601 cactttccct tctcgaaagg ttgttaaaca gtacacgggt taatacacag ccggataata
    18661 agcttgcggc gttgttatgc gcgcggataa atggcagtcc tgcttgtttt gccgcctgta
    18721 tggattcatc aaatgaatat aaaaaaatca gcgcccttgt gcgcaagggc gaaaatgaaa
    18781 ttaaccaatg ggctgaccgt cattctgttg agcacgctac cgttcaggcg atacaatggc
    18841 tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa
    18901 aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc
    18961 gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa
    19021 aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg
    19081 ctagcgcagc ttaaacagca actgcattct cagacgccac gcgctgaagg ggtggtaaaa
    19141 gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg
    19201 ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat ccacagtgaa
    19261 aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg
    19321 ggtaaagttc agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa
    19381 gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga aggcgactgg
    19441 gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta tgctgaactg
    19501 acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc
    19561 cataatctgg aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg
    19621 gttcgtgaag atctgaccgc gctggatttt gtcaccatcg acagtgccag cacagaagat
    19681 atggatgacg cccttttcgc taaggcgttg ccggatgaca aacttcagct gattgtggcg
    19741 attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt
    19801 cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct
    19861 gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg
    19921 ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac catcgaatcc
    19981 aaagcgaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg tgactggcag
    20041 cctgaaagtg aagcaattgc cgaacaagtc cgtttgttag cgcaaatttg ccaacgccgc
    20101 ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta ccgctttatt
    20161 ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt
    20221 atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg cccgcgtact gcgcgataag
    20281 ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg
    20341 gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct cacgctggac
    20401 ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct cgacagccgc
    20461 attcgtcgct tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc
    20521 ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac
    20581 caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc
    20641 actgtccaaa tggccgagcg tcgccgtctc aaccggatgg cagaacgtga tgttggcgac
    20701 tggttatacg cacgcttcct gaaagacaaa gccgggaccg acacccgttt cgcggcggaa
    20761 attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc
    20821 tttattccgg cacctttctt acacgctatg cgcgatgaac tggtttgcag ccaggaaaac
    20881 ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc
    20941 attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct
    21001 ttcacggccc attcctcatg gatgggccgt ttatttcccc gctctacctt cgttatatcc
    21061 aggcagattt tttaactgac tttcgtttga aaactggcgt ttttccaaaa ctggattaca
    21121 aataaatata taaaaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg
    21181 ttccttttat ttaaccgcgg actccgctgt taaccggagg atatgcatca tgaaaaccgt
    21241 tagggagtcc acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac
    21301 ggaaagcagc gatgttttgc gcttttctac caccgaaacc acagaacctg atcgtacatt
    21361 gcagttatct gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct
    21421 gatgatgagt ctgaccgtcg atgaaagcga tctttctgtg catctggtag gacgaaaaat
    21481 caataaacgg gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg
    21541 tgatttatca cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat
    21601 agtgattctc gacagccggg ggaatatcca acgcttcaat cggttatgtg aagattacac
    21661 agggttgaaa gaacacgacg tcattgggca aagcgtgttt aaactgttta tgagccgtcg
    21721 tgaagctgcg gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga
    21781 agtcgaactg tggataccaa catgtaaagg ccagcggctg tttctgtttc gcaataaatt
    21841 tgtccacagc ggcagtggca aaaacgagat ttttttaatc tgttccggca ccgacattac
    21901 cgaagagcgc cgcgctcagg agcgactgcg tattctggca aataccgaca gtatcaccgg
    21961 actgccgaat cgtaacgcaa tgcaggattt aatcgatcac gctattaatc atgcagataa
    22021 caataaagtt ggggttgtgt atcttgattt ggataatttc aaaaaggtca acgacgccta
    22081 tgggcatttg tttggtaacc agttattacg cgacgtgtca ttggctattt taagctgtct
    22141 cgaacatgac caggtgttgg cgcgtccagg tggcgatgag tttctggtac tggcatccaa
    22201 tacctcacaa agcgcgctgg aagcaatggc atcacgaatt ttgacccgct tacgcctccc
    22261 ctttcgtatt ggtttaattg aagtttatac cagctgttca gtaggtattt cgctctctcc
    22321 cgaacatggt tcggacagcg cgactattat tcgtcacgcc gacacagcaa tgtacacagc
    22381 gaaggaaggc ggacgaggac aattttgcgt ttttactcca gaaatgaatc agcgggtatt
    22441 tgaatatctc tggctggata ccaacttgcg taaagcactg gaaaacgatc agttggttat
    22501 tcactatcaa ccgaaaatca cctggcgtgg cgaagtgcgc agtctggaag cactagtacg
    22561 ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct acgccgaaga
    22621 gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac gccaggtggc
    22681 aaagtggcgg gataaaggca taaacctgcg agtggcggta aatatttctg cacgtcagct
    22741 cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca attttgaata
    22801 ctgccctata gatgttgaac tgacagagag ttgtctgatt gagaatgatg aactggcact
    22861 gtctgttatt caacaattta gccaactagg tgcgcaagtg catctggatg attttggtac
    22921 cggctactct tcactttcgc aactggcgcg ctttccgatc gatgccatca aacttgacca
    22981 ggtttttgtt cgagatattc acaaacaacc tgtctcgcag tcactggtcc gggcgatcgt
    23041 cgctgtggcc caggcattga atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga
    23101 agatgctttt ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt ttgccaaacc
    23161 gatgcccgcc gtcgccttcg aacgctggta taaacgctat ctgaagcgcg cataagtaga
    23221 tgaatatata tacttagcgc tatatcccg
//
LOCUS       DAZFAH010000057        23187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFAH010000057 DAZFAH010000000
VERSION     DAZFAH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.057"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1050
                     /locus_tag="ACHJWB_002113"
     CDS             <1..1050
                     /locus_tag="ACHJWB_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4313751.1"
                     /translation="HKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
     gene            1231..2721
                     /gene="dgcJ"
                     /locus_tag="ACHJWB_002114"
     CDS             1231..2721
                     /gene="dgcJ"
                     /locus_tag="ACHJWB_002114"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO4313752.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQAGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2764..3267
                     /gene="yeaK"
                     /locus_tag="ACHJWB_002115"
     CDS             2764..3267
                     /gene="yeaK"
                     /locus_tag="ACHJWB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4313753.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFHVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDELAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3268..3372)
                     /gene="yoaI"
                     /locus_tag="ACHJWB_002116"
     CDS             complement(3268..3372)
                     /gene="yoaI"
                     /locus_tag="ACHJWB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4313754.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3542..3988
                     /gene="yeaL"
                     /locus_tag="ACHJWB_002117"
     CDS             3542..3988
                     /gene="yeaL"
                     /locus_tag="ACHJWB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4313755.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3945..4766)
                     /gene="nimR"
                     /locus_tag="ACHJWB_002118"
     CDS             complement(3945..4766)
                     /gene="nimR"
                     /locus_tag="ACHJWB_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4313756.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4863..6044
                     /gene="nimT"
                     /locus_tag="ACHJWB_002119"
     CDS             4863..6044
                     /gene="nimT"
                     /locus_tag="ACHJWB_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4313757.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSKAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6099..6446
                     /gene="yeaO"
                     /locus_tag="ACHJWB_002120"
     CDS             6099..6446
                     /gene="yeaO"
                     /locus_tag="ACHJWB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4313758.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6468..6722)
                     /gene="yoaF"
                     /locus_tag="ACHJWB_002121"
     CDS             complement(6468..6722)
                     /gene="yoaF"
                     /locus_tag="ACHJWB_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4313759.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6905..7930
                     /gene="dgcP"
                     /locus_tag="ACHJWB_002122"
     CDS             6905..7930
                     /gene="dgcP"
                     /locus_tag="ACHJWB_002122"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO4313760.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7963..8061
                     /gene="yoaK"
                     /locus_tag="ACHJWB_002123"
     CDS             7963..8061
                     /gene="yoaK"
                     /locus_tag="ACHJWB_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4313761.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8064..8138
                     /gene="yoaJ"
                     /locus_tag="ACHJWB_002124"
     CDS             8064..8138
                     /gene="yoaJ"
                     /locus_tag="ACHJWB_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4313762.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8197..8445)
                     /gene="yeaQ"
                     /locus_tag="ACHJWB_002125"
     CDS             complement(8197..8445)
                     /gene="yeaQ"
                     /locus_tag="ACHJWB_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4313763.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8593..8775)
                     /gene="yoaG"
                     /locus_tag="ACHJWB_002126"
     CDS             complement(8593..8775)
                     /gene="yoaG"
                     /locus_tag="ACHJWB_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4313764.1"
                     /translation="MGKATYTVTVTNNSNGVSIDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8779..9138)
                     /gene="yeaR"
                     /locus_tag="ACHJWB_002127"
     CDS             complement(8779..9138)
                     /gene="yeaR"
                     /locus_tag="ACHJWB_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4313765.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9311..9949)
                     /gene="leuE"
                     /locus_tag="ACHJWB_002128"
     CDS             complement(9311..9949)
                     /gene="leuE"
                     /locus_tag="ACHJWB_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4313766.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10076..10999)
                     /gene="dmlR"
                     /locus_tag="ACHJWB_002129"
     CDS             complement(10076..10999)
                     /gene="dmlR"
                     /locus_tag="ACHJWB_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4313767.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11102..12187
                     /gene="dmlA"
                     /locus_tag="ACHJWB_002130"
     CDS             11102..12187
                     /gene="dmlA"
                     /locus_tag="ACHJWB_002130"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4313768.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12438..14048
                     /gene="yeaV"
                     /locus_tag="ACHJWB_002131"
     CDS             12438..14048
                     /gene="yeaV"
                     /locus_tag="ACHJWB_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4313769.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14080..15204
                     /gene="yeaW"
                     /locus_tag="ACHJWB_002132"
     CDS             14080..15204
                     /gene="yeaW"
                     /locus_tag="ACHJWB_002132"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4313770.1"
                     /translation="MSNLSPDFVLPENFCTNPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15260..16225
                     /gene="yeaX"
                     /locus_tag="ACHJWB_002133"
     CDS             15260..16225
                     /gene="yeaX"
                     /locus_tag="ACHJWB_002133"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4313771.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFTIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16279..17394)
                     /gene="rnd"
                     /locus_tag="ACHJWB_002134"
     CDS             complement(16279..17394)
                     /gene="rnd"
                     /locus_tag="ACHJWB_002134"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4313772.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17476..19161)
                     /gene="fadD"
                     /locus_tag="ACHJWB_002135"
     CDS             complement(17476..19161)
                     /gene="fadD"
                     /locus_tag="ACHJWB_002135"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4313773.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTCDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19366..19947)
                     /gene="yeaY"
                     /locus_tag="ACHJWB_002136"
     CDS             complement(19366..19947)
                     /gene="yeaY"
                     /locus_tag="ACHJWB_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4313774.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(19987..20682)
                     /gene="tsaB"
                     /locus_tag="ACHJWB_002137"
     CDS             complement(19987..20682)
                     /gene="tsaB"
                     /locus_tag="ACHJWB_002137"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4313775.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20740..22650)
                     /gene="yoaA"
                     /locus_tag="ACHJWB_002138"
     CDS             complement(20740..22650)
                     /gene="yoaA"
                     /locus_tag="ACHJWB_002138"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4313776.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22782..23126
                     /gene="yoaB"
                     /locus_tag="ACHJWB_002139"
     CDS             22782..23126
                     /gene="yoaB"
                     /locus_tag="ACHJWB_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4313777.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5677 a         5920 c         5633 g         5957 t
ORIGIN      
        1 cataaaccaa ttaacggcag tacactgatc cagacaaagt cgaatgatgt ttctattttc
       61 tatattttcc gccaactcag ttttatttgt ttaacctcgc tggcactctt ttgttatgga
      121 aaagacaaca tccttgacaa caataagaaa aaaacgggaa tcctgttgct ggcgctgatt
      181 ccttttttag tttttcccct tctggcacac aatctgagca gttataacgc tgactattct
      241 ttgtatgtcg tcgactactg tccggacaac catactgcga cctggggaat caactataca
      301 aaaatattgg tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta
      361 gccagcgaac tatggccgtt aatagcatta ttatgtctgg catcgctatg ctgcaactta
      421 cttctactga ctctggatga gtataattac accatctggt atatcagtcg cgggattgaa
      481 gtttccagta aactgtttgt tgtgtctttt ctgatttata acatttttca ggaactgcaa
      541 ctctccagca aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt
      601 ttcaacagcg tagagtcatt attgtcgcga cctgttgtta aggacttctg tgtcatgctg
      661 gttgatatta atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa
      721 gtgctggttt caattgtcga tattatccag caaagcatcc gccctgatga tattttagcg
      781 cgactggagg gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa
      841 atcattgcgg aacgtatgcg taaaaatgtc gaactcctga ccggctttag taacagatat
      901 gatgttcctg aacaaatgac catcagtatt ggcacggttt tttcaacggg tgacacgcgt
      961 aatatctcgc ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg
     1021 ggcaacaaag tgattattca tcatatttaa gtgcaaaaat attcagagcc atgctttttg
     1081 cgtggctttt gcatacaatt tattactatt acccctaaat ttcccctccc cagcggcgca
     1141 gagatgagta taattagcgc ccctgtgcca ggccgcaatc gaactttatc tggttttctc
     1201 gtttcactaa ccgaaggagt gccatttatc atgaaattgc accatagaat gctccggcat
     1261 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
     1321 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
     1381 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
     1441 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
     1501 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
     1561 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
     1621 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
     1681 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
     1741 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
     1801 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
     1861 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
     1921 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
     1981 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
     2041 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
     2101 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
     2161 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
     2221 cggatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     2281 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     2341 caagccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     2401 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     2461 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     2521 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     2581 caacatatcg cgccgcagaa agagattggc ttctcttccg gtatttacgc gatgaaagaa
     2641 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     2701 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     2761 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     2821 caagaaggtg ctaacttcca cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     2881 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     2941 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     3001 agccaactcg ccagccatat aggcggatta cgcgcttcgt tggccagccc ggcggaagtt
     3061 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     3121 aaactggttg ccgaccccct actcttcgaa cgatttgatg aactcgcctt caatgcaggc
     3181 atgctggata aatccgttat tttgaaaacc gcggattatt tgcgcattgc gcaaccagaa
     3241 ctggtcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
     3301 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
     3361 ttgatcgttc atagcatgcc cccgggatat agctgttttc acgttattgt tagcgtgcac
     3421 aaatggcagt ttgatgacag ttcgcctgat tttttatgca aaaaacgtaa agattttcta
     3481 cttccttcct gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac
     3541 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
     3601 taacaccact gtcgccgttt caattctggt gttaatcatt gtccgcgtca caccgttaag
     3661 cacttttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
     3721 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccctga ttcactcgtt
     3781 tctgaactgg aaatcactgg tggcaattgc agtaggcgtt attgtctcct ggctgggtgg
     3841 gcgcggcgtg acgttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac
     3901 tgttttaggc gtggcgctgt ttcgcggcgt accggttggc ccgcttattg ccgcgggtct
     3961 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
     4021 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
     4081 gcaactttct gtacagtatc gcctttcact aatccctgaa gcgccataat cagctgtaac
     4141 tgctgacgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg ggcaagattg
     4201 cgttcactca tcgcaaaaaa gccagcccat tgccccaatg acccccactc gacgggccct
     4261 ttcgccatca tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc
     4321 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatt
     4381 ggctcagcac gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag
     4441 ggcgatattt tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcaataaac
     4501 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
     4561 caaaccgcat attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca
     4621 ccatgtagcg ccagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
     4681 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
     4741 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
     4801 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
     4861 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
     4921 tgatgattgc cacaacatta cgcgtcacct ttaccggcgc agcaccgtta ctggatacga
     4981 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat
     5041 tggcctttgc gctaatatca cctttggccg ccccggtagc gcgacgtttt ggtatggaac
     5101 gtagcctgtt tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt
     5161 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
     5221 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
     5281 catattccct gacaatggga gctgcggcgg cacttggatc ggctatggta gtgccgctgg
     5341 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
     5401 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
     5461 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc
     5521 ttgggatcaa ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca
     5581 gtcacggcta tagcaaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
     5641 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
     5701 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
     5761 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
     5821 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
     5881 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg
     5941 atgctaacgg taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg
     6001 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
     6061 gacgtgacaa atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg
     6121 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
     6181 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
     6241 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
     6301 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
     6361 aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
     6421 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
     6481 gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
     6541 caattgttgc tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg
     6601 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtgc ttctggctga
     6661 gaagaggttg aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc
     6721 atttattgat ctcactcatt tatctacgct tagagtatcg cggtatcgtt ctgttttgca
     6781 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttggtga
     6841 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
     6901 ttttgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
     6961 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
     7021 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
     7081 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
     7141 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
     7201 tgcggcacgc aatcttggca tcaccacatt tctgagcacg ccaattcact taccggatgg
     7261 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
     7321 ggaacaggtt ttacagttat tcgccggact tattgcacaa tatattcaaa aagaggcact
     7381 ggttgaacag ctgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
     7441 aaccgggcta ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt tttccctcgc
     7501 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
     7561 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
     7621 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggtggtgatg agtttttggt
     7681 tgtttcactg aacaacgaga atgcggatat ttcgtcgctt cgagaacgta ttcagcagca
     7741 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
     7801 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
     7861 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc atccagcgct
     7921 acattcctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
     7981 tttccggttg taatcttcat tacagcggtc gtatttttag catggttttt tattggcggc
     8041 tatgctgctc cgggggcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
     8101 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
     8161 aggctgcctg tgtgcagcct ttgtgcaatt tacgcgttaa cttttaatct tcctgtagat
     8221 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
     8281 gactttacca aagccaaaca gcgtgctgat ccatccgccg actacagcac cgactatccc
     8341 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
     8401 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
     8461 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
     8521 gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac
     8581 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
     8641 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     8701 cgtctctgtt tcataatcga tagaaacgcc attactgtta ttggtgacgg tcacggtata
     8761 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     8821 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     8881 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
     8941 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     9001 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     9061 tatgccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     9121 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
     9181 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     9241 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     9301 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     9361 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     9421 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     9481 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     9541 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     9601 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     9661 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     9721 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     9781 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     9841 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     9901 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     9961 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
    10021 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaata attaatcagg
    10081 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
    10141 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
    10201 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
    10261 cccaccagga acgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
    10321 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
    10381 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcagaaa
    10441 gatcggttaa atgtttcggc gcaccatgtt gcgcaataaa ttccggcgac gcgcagagaa
    10501 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg tgcgatgtca tcgccaatgc
    10561 gaatatcgag atcgacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
    10621 gtaattccag ttgcggatac gccttcgcca gcgctgataa cgccggagcc accacctgtc
    10681 gtccaaaacc aaagctgctg ataatacgca gcatcccctg gggcacctga cgcacgtcag
    10741 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
    10801 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
    10861 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
    10921 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcgttca
    10981 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
    11041 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
    11101 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
    11161 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg ccttgagttt
    11221 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    11281 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    11341 ggacaccgtt cctgaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    11401 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    11461 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    11521 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    11581 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    11641 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    11701 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagattc gctgggacaa
    11761 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    11821 ggtggcgtcc aatttatttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    11881 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
    11941 gccagtccac ggttccgcgc cggatatcta cgggaaaaac attgctaacc ctatcgccac
    12001 gatttgggcc ggtgcaatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    12061 agcgcataac ggtattctgg cagcaattga agaagtgatt gctcacgggc cgaaaacacc
    12121 tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    12181 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
    12241 tttgcagtaa ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    12301 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
    12361 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    12421 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    12481 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    12541 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
    12601 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
    12661 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    12721 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
    12781 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
    12841 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
    12901 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
    12961 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
    13021 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    13081 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    13141 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
    13201 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggcc
    13261 caaccgaatt tattaccaac agcatcatca atgccatcgg tctgaccacg caaaacttcc
    13321 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    13381 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
    13441 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    13501 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    13561 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
    13621 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    13681 tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt acgcgtaatc
    13741 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    13801 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    13861 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    13921 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    13981 caccggttga agccctggaa aaaacgccag tgctccctgc gggaaccgta ttcaaaggcg
    14041 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    14101 tttgtactac ccgaaaattt ttgcactaac ccgcaagagg cgtggaccat tcctgcccgt
    14161 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    14221 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    14281 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    14341 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    14401 acctgcccgt atcacgcatg ggcattcaaa ctcgatggca acctggccca tgcacgtaac
    14461 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
    14521 gaatatgctg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcaa
    14581 ttacccggcc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    14641 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    14701 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
    14761 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    14821 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    14881 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    14941 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    15001 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    15061 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    15121 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
    15181 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
    15241 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagccaggt
    15301 tgaacccctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccatt
    15361 acctgcgttt accggaggaa gtcacgtcat tgtgcagatg agcgatggtg ataaccagta
    15421 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
    15481 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    15541 caatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    15601 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagct
    15661 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    15721 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gttcacttgc atcactcatc
    15781 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    15841 ttatacctgt ggtcccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
    15901 catcgccgcc gatacgctgc actttgagca atttactatc gaagacaaaa ccggcgatgc
    15961 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    16021 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    16081 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
    16141 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    16201 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
    16261 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    16321 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    16381 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    16441 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    16501 accaggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    16561 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    16621 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
    16681 ctcttcacgc acgacaaagt tcaccgccag atcgcgctct cgcgccttgc gcagtcgcca
    16741 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    16801 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
    16861 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    16921 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
    16981 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    17041 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    17101 gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    17161 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    17221 gtcggtgatc ccgagtggat cgattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
    17281 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
    17341 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    17401 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    17461 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    17521 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    17581 cggtactttg tatcccgtga gctgacggcg gcaaaaagtc accagtgact cttcggtaag
    17641 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    17701 gccaacagcc gcgacctcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    17761 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcaa caatgcgcag
    17821 gaatccttct tcatccatta ccgcgatgtc gcaggtgtgt aaccagccat ttttgatgat
    17881 ttcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    17941 aagctcaccc ggttgacctg gtggtacttc attatcatca tcatccacca gtttggcttc
    18001 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    18061 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
    18121 tttcacccaa cgctctgcca ccacttgctg cactggcatc cctccgcctg cggaaagatg
    18181 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    18241 gttaacgccc gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    18301 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    18361 aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt ctttacctgg
    18421 atgcagcaac ggcccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    18481 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    18541 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    18601 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    18661 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    18721 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    18781 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    18841 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    18901 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    18961 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    19021 ctcccccata ttcacaaacg caggctgatc ggcgtagcgc gcgaccgact gctcaaacat
    19081 atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg cgggataacg
    19141 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    19201 cccaaatgat atacatgccg ttaacataat attaactcat cataccagct tgataattac
    19261 ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt aagaatgcag
    19321 aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt cagttacaac
    19381 tgtttgtact ctcgcgggac cgggattata ccagccccat ccgccgtagc catagggcca
    19441 gccacgaccg ccataaaacc atggatcaat cggctgaggc ggcataatca cctgctgggt
    19501 taaatgccaa cgtttgtaac ccgttacttg catcaccata aatttatagg gcgtattgcc
    19561 gattttgccg tcaaccgcac cagtgattgg cccgactacc gtaaccagtt gtccacggaa
    19621 atccaccggg tccagaaaac cgttcacatc ggcataaatg cgaccgcgag aaggttctcc
    19681 cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    19741 ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac caacgtacag
    19801 ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc tgcctttaat
    19861 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    19921 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    19981 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    20041 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    20101 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    20161 accgagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    20221 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgtgcca
    20281 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    20341 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag
    20401 cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc agcccttgcg caatgccaat
    20461 accaatgcgc acgccggtaa agctaccggg gccgcgcccg taagccagag cgttaatatc
    20521 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    20581 atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc acagggcgac
    20641 agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    20701 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    20761 aaggaaacga accgcacggg caatgtcacg agtgcgtggc gcgggcggca gactggcgag
    20821 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaatcacca gcacgccacg
    20881 atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg agagtaatga cggcatctgg
    20941 tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    21001 taacagtgga tcatccggcg aggtaaacgg caatttgtcg ataattacca atgacaatgt
    21061 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    21121 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    21181 aagcgtcatg gtagcgcgga actgctcggc cagatcacgc atcatggcgt gcgaggtaca
    21241 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgctaactg
    21301 gcgagcagaa cctggctggt tggtttgcgg cagattgcgc ggcacacaga gtaacgcctg
    21361 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    21421 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    21481 accaggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    21541 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    21601 ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc tcaaatgccg catccagcaa
    21661 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    21721 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    21781 acctggctca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca
    21841 cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa gcgatggtga tgtcttttgc
    21901 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    21961 tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcaact cgccaaatcc
    22021 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    22081 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    22141 gccaagacag ttgtcgttgg tgctggtaac cagcggccac gcctgtgaat cttccgccac
    22201 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    22261 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    22321 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    22381 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    22441 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    22501 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    22561 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    22621 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    22681 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    22741 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    22801 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    22861 taccggaaaa cctcgacgcc gatgcttttg agcaaaccgc caacacgctg gcgcagattg
    22921 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    22981 tggctgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtggcgg
    23041 gtcatgcgcc ggtgcgctgc acggtgcaag cggggctgat gaacccgaag tataaagttg
    23101 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    23161 tatcgataat actggtcggt tttacat
//
LOCUS       DAZFAH010000058        22842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFAH010000058 DAZFAH010000000
VERSION     DAZFAH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.058"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            96..1388
                     /locus_tag="ACHJWB_002140"
     CDS             96..1388
                     /locus_tag="ACHJWB_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4313778.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKRDELIEQH"
     gene            1409..2290
                     /locus_tag="ACHJWB_002141"
     CDS             1409..2290
                     /locus_tag="ACHJWB_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4313779.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2277..3119
                     /locus_tag="ACHJWB_002142"
     CDS             2277..3119
                     /locus_tag="ACHJWB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4313780.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3150..4202
                     /locus_tag="ACHJWB_002143"
     CDS             3150..4202
                     /locus_tag="ACHJWB_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4313781.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILSNEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDGNVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4220..5008
                     /locus_tag="ACHJWB_002144"
     CDS             4220..5008
                     /locus_tag="ACHJWB_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4313782.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            5018..6073
                     /locus_tag="ACHJWB_002145"
     CDS             5018..6073
                     /locus_tag="ACHJWB_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4313783.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6070..8337
                     /gene="ycjT"
                     /locus_tag="ACHJWB_002146"
     CDS             6070..8337
                     /gene="ycjT"
                     /locus_tag="ACHJWB_002146"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4313784.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLMETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAQV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVHDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDY
                     ILPFNRTATTHQEDE"
     gene            8334..8993
                     /gene="pgmB"
                     /locus_tag="ACHJWB_002147"
     CDS             8334..8993
                     /gene="pgmB"
                     /locus_tag="ACHJWB_002147"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4313785.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            9007..10089
                     /locus_tag="ACHJWB_002148"
     CDS             9007..10089
                     /locus_tag="ACHJWB_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4313786.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10134..11039
                     /gene="ompG"
                     /locus_tag="ACHJWB_002149"
     CDS             10134..11039
                     /gene="ompG"
                     /locus_tag="ACHJWB_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4313787.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11150..12148)
                     /locus_tag="ACHJWB_002150"
     CDS             complement(11150..12148)
                     /locus_tag="ACHJWB_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4313788.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            12303..13700
                     /locus_tag="ACHJWB_002151"
     CDS             12303..13700
                     /locus_tag="ACHJWB_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4313789.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13697..14758
                     /locus_tag="ACHJWB_002152"
     CDS             13697..14758
                     /locus_tag="ACHJWB_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4313790.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14906..16447
                     /gene="tyrR"
                     /locus_tag="ACHJWB_002153"
     CDS             14906..16447
                     /gene="tyrR"
                     /locus_tag="ACHJWB_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4313791.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16491..16997)
                     /gene="tpx"
                     /locus_tag="ACHJWB_002154"
     CDS             complement(16491..16997)
                     /gene="tpx"
                     /locus_tag="ACHJWB_002154"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4313792.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17116..18081
                     /gene="ycjG"
                     /locus_tag="ACHJWB_002155"
     CDS             17116..18081
                     /gene="ycjG"
                     /locus_tag="ACHJWB_002155"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4313793.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18056..18784)
                     /gene="mpaA"
                     /locus_tag="ACHJWB_002156"
     CDS             complement(18056..18784)
                     /gene="mpaA"
                     /locus_tag="ACHJWB_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4313794.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19094..19732)
                     /locus_tag="ACHJWB_002157"
     CDS             complement(19094..19732)
                     /locus_tag="ACHJWB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4313795.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKCTIVSRKSVAALITDIIDKPEKHIGENIGINQSVTDGDKPLFM"
     gene            complement(19750..20682)
                     /locus_tag="ACHJWB_002158"
     CDS             complement(19750..20682)
                     /locus_tag="ACHJWB_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4313796.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(20682..21602)
                     /locus_tag="ACHJWB_002159"
     CDS             complement(20682..21602)
                     /locus_tag="ACHJWB_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4313797.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            21740..22639
                     /gene="pgrR"
                     /locus_tag="ACHJWB_002160"
     CDS             21740..22639
                     /gene="pgrR"
                     /locus_tag="ACHJWB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4313798.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         5478 a         5548 c         6149 g         5667 t
ORIGIN      
        1 tggtcgggat ctgattagtg aagttgatat attgggtaat gaaataacgc tgcgcccctg
       61 gcaggttatg tggattaaat aaaaaggaac atctcatgat taaatcaaaa atcgtgctgt
      121 tatcagcact ggtttcctgc gccctgattt caggctgtaa agaagaaaat aaaacgaatg
      181 tatccatcga atttatgcat tcttcggtgg agcaggagcg ccaggccgtt atcagtaaat
      241 tgattgcccg ttttgaaaaa gaaaaccctg gcatcaccgt taagcaagtg cccgtggaag
      301 aagatgccta taacactaaa gtcattactc tttcacgtag cggttcgctg ccggaagtga
      361 tagaaaccag ccatgactac gccaaagtga tggacaaaga gcagcttatc gatcgcaaag
      421 cggttgccac agtcatcagc aacgttggtg aaggcgcgtt ttacgatggc gtactgcgta
      481 ttgtgcgtac cgaagatggt agcgcatgga ccggtgttcc tgtcagcgcc tggattggcg
      541 gtatctggta tcgcaaagat gtgctggcaa aagcggggct tgaggagccg aaaaactggc
      601 aacagctgct ggacgttgca cagaaactga atgacccggc gaataaaaaa tacggcattg
      661 cgctgcctac agcagaaagc gtgttgacgg aacaatcctt ctcccagttt gcgttatcca
      721 accaggctaa cgtctttaac gccgaaggca aaatcaccct tgatacacca gagatgatgc
      781 aggcactgac ctattaccgc gaccttgctg ccaacactat gccgggttct aacgacatca
      841 tggaagtgaa agacgccttt atgaacggca ccgcgccgat ggcgatttac tccacctata
      901 tccttccggc tgtgattaaa gaaggcgacc cgaaaaacgt cggtttcgtg gtgccaaccg
      961 agaaaaactc tgcggtctac ggcatgttga cctcgctgac cattaccgcc gggcaaaaga
     1021 ccgaagagac ggaagcagca gaaaaatttg tcacctttat ggagcaggca gacaacattg
     1081 ccgactgggt gatgatgtcg ccaggtgctg cactgccggt gaataaagcg gttgtcacta
     1141 ccgccacctg gaaagacaac gacgttatta aggcgctggg tgaactaccg aatcagctaa
     1201 tcggtgaact gccaaatatt caggtctttg gtgcagtagg ggataaaaac tttacccgca
     1261 tgggtgatgt gacgggttct ggcgtggtga gctccatggt gcataacgtc accgtgggta
     1321 aagccgatct ccctggcacg ctgcaagcga gccagaaaaa gcgggatgaa ctgatcgaac
     1381 agcactaaac ccaggacagg aatccgcaat gaacaggctt ttttcaggtc gttccgatat
     1441 gccctttgcg ctgctgcttc tcgcgcccag cttattactg ctgggtggtc tggtggcctg
     1501 gccaatggtg tcgaatatcg aaatcagttt tttacgtctg ccgctcaatc ccaacatcga
     1561 gtcaaccttt gttggggtga gtaactatgt gcgtatcctc tccgatcccg gcttctggca
     1621 ctcgctgtgg atgacggtct ggtataccgc gctggtggtg gcgggcagca ccgtgctcgg
     1681 gctggcagtg gcgatgtttt tcaaccgcga gttccgcctg cgcaaaaccg cgcgttcgct
     1741 ggtgatcctc tcctacgtaa cgccgtccat ttcgctggtg tttgcctgga aatacatgtt
     1801 caacaacggc tacggcattg tgaactacct cggcgtcgat cttctgcatc tctatgagca
     1861 ggcaccactg tggttcgata atccgggcag tagctttgtg ctggtggtgc tgttcgccat
     1921 ctggcgctac ttcccgtatg cctttatctc gtttctggcg attttgcaga ccatcgacaa
     1981 atcgctgtac gaagcggcag agatggatgg cgctaatgcc tggcaacggt ttcgtatcgt
     2041 cacgctgccc gcgattatgc cggtcctggc gacggtggtg accctgcgca ccatctggat
     2101 gttctacatg ttcgcggatg tttatttgct gaccaccaag gtcgatattc tcggtgtata
     2161 tctctacaaa accgcctttg cctttaatga tttaggaaaa gcggcggcga tctcggtggt
     2221 gctcttcatc attattttcg ctgtcattct gctgaccagg aaacgggtga acctcaatgg
     2281 caacaaataa acgcacactc agtcgcatcg gtttttactg cgggctggcg ctgtttctca
     2341 tcatcacgct gtttccattt tttgtgatgc tgatgacctc gttcaagggc gcgaaagagg
     2401 cgatctcact gcatcctacg ctgctgccgc agcagtggac gctggagcat tacgtcgaca
     2461 tttttaaccc gatgattttc ccgtttgtcg actacttccg taacagtctg gtggtgtcgg
     2521 tggtttcatc cgtggtggcg gtattcctcg gcattcttgg ggcttacgcg ctttcccgcc
     2581 tgcgctttaa aggtcggatg accatcaatg ccagctttta cacggtgtat atgttctctg
     2641 gcattttgct ggtggtgccg ctgttcaaaa tcatcaccgc gctgggcatt tacgacaccg
     2701 agatggcgct gatcatcacc atggtgacgc aaactctgcc caccgccgtg tttatgctga
     2761 aaagctactt cgacaccatc cccgatgaga tcgaagaagc ggcgatgatg gatggcctca
     2821 accgtctgca aattatcttc cgcatcaccg tgccgttggc gatgtccggg ctgatatccg
     2881 tgttcgtcta ctgctttatg gtggcgtgga acgactatct gtttgcgtcg attttcctct
     2941 ccagcgccag caatttcacc ttaccggtgg gcctgaacgc gctgttcagt acgccagatt
     3001 acatctgggg acgcatgatg gcggcctcgc tggtgaccgc attgccggtg gtgattatgt
     3061 atgcgctttc ggaacgtttt attaagagtg gtttgaccgc cggtggcgtg aagggctaaa
     3121 gcggccagtt tctttacaag gagttttaca tgaaaaagtt agtagccaca gcaccgcgtg
     3181 ttgctgcgct ggttgagtat gaagatcggg cgattttatc caatgaagtg aagatccgcg
     3241 tgcgtttcgg cgcaccgaaa cacggaacgg aagtggtcga cttccgcgcc gccagcccgt
     3301 ttatcgatga agactttaac ggcgagtggc agatgttcac cccgcgtccg gcagatgcgc
     3361 cgcgcggcat tgagtttggc aaattccagc ttggaaacat ggtggttggc gacattatcg
     3421 agtgcggcag cgacgttacc gactacgcgg tgggcgacag cgtatgcggc tacggcccgc
     3481 tctccgagac ggtcatcatt aacgcggtga ataactacaa gctgcgcaaa atgccggaag
     3541 gcagctcgtg gaaaaacgcc gtttgttacg acccggcgca gtttgccatg agcggcgtgc
     3601 gcgatggcaa cgtgcgcgtg ggtgattttg ttgtggtggt agggcttggc gcgattggtc
     3661 aaattgccat tcaactggcg aaacgcgctg gcgcgtcggt cgtgattggc gtcgatccta
     3721 tcgcccatcg ctgtgatatt gcccgtcgcc acggcgcgga tttctgcctt aaccccattg
     3781 gtactgacgt aggcaaagag atcaaaacgc tgaccggcaa gcagggtgcc gatgtgatta
     3841 tcgaaaccag cggctatgcc gacgcgctgc aatcggcgtt ccgcggtctg gcctacggtg
     3901 gcaccatctc ctatgtcgcg tttgccaaac catttgctga aggttttaac ctcggacgcg
     3961 aagcgcattt caataacgcc aaaattgttt tctcccgcgc gtgcagcgaa ccgaacccgg
     4021 attatccgcg ctggagccgt aagcgtattg aagaaacctg ctgggaactg ctgatgaacg
     4081 gttatctcaa ttgcgaagat ttaatcgacc cggtagtgac ctttgccaag agcccggaaa
     4141 gctacatgca gtatgtcgat cagcatccgg aacagagcat caaaatgggc gttacgtttt
     4201 aattcaaagg aatgaaaaaa tgaaaatagg cacacagaat caggcattct ttccggaaaa
     4261 cattctggag aaatttcgtt atatcaaaga gatgggcttc gatggttttg agattgacgg
     4321 caaactgctg gtcaacaatc tcgaagaagt caaagcagca atcaaagaaa ccggtttacc
     4381 ggtgaccacc gcctgcggtg gctatgacgg ttggattggc gactttatcg aagagcgtcg
     4441 tcttaatggc ttaaagcaga tcgaacgcat tctcgaagcg ctggcagaag tggggggtaa
     4501 aggtatcgtc gttccggcag cgtggggcat gtttaccttc cgcttaccgc cgatgacctc
     4561 gccgcgtagc ctggacggcg accgcaaaat ggtgagtgat tccctgcacg tactggaaca
     4621 ggtcgccgcg cgtaccggaa ccgtggtgta tctcgaaccg ttaaaccgct atcaggatca
     4681 tatgatcaac accctcgccg atgcccgccg ttacatcgtc gaaaacgatc ttaaacatgt
     4741 acagattatc ggcgatttct atcacatgaa tatcgaagaa gataacctgg cgcaggcgct
     4801 gcatgacaac cgcgacctgc tcggtcatgt gcatattgcg gataaccatc gctaccagcc
     4861 gggcagcggc accctggatt tccacgcgct gtttgaacag ctgcgcgcgg ataactatca
     4921 gggctatgta gtgtacgaag ggcgtatccg ggcggaagat cctgcccagg cgtaccgtga
     4981 ttcgttggcc tggttgcgta cctgctaaga ggtctttgtg aaaagtgcaa tgacaagctc
     5041 tccgctgcgg gtcgcgataa taggcgcagg ccaggtggcg gataaggttc atgcttcgta
     5101 ctactgcacc cgcaacgatc tggaactggt ggctgtctgt gacagccgcc tttcccaggc
     5161 gcaggcgctg gcagataaat acggtaatgc atccgtgtgg gacgatccgc aggccatgct
     5221 gctggcggtg aaacctgatg tggttagcgt ctgctcacct aaccgttttc attacgaaca
     5281 taccctgatg gcactggaag cgggctgcca tgtgatgtgc gaaaaaccgc ccgccatgac
     5341 gccagaacag gcgcgggaaa tgtgcgatac cgcgcgcaaa ctgggcaagg tgctggccta
     5401 cgactttcac catcgttttg cgctcgatac gcaacagctg cgtgaacagg tgaccaacgg
     5461 cgttttggga gagatttacg ttaccaccgc ccgcgccctg cgtcgctgcg gcgttcccgg
     5521 ctggggcgtc tttaccaata aagaactgca gggtggtggc ccgctgatcg acatcggcat
     5581 tcatatgctg gatgctgcga tgtatgtgct gggctttccg gcggtgaaaa gcgtgaatgc
     5641 gcatagcttt caaaagatcg gcacgcaaaa gagctgtggt caatttggcg agtgggatcc
     5701 ggcaacttat agcgtcgaag attcgctgtt tggcaccatt gaatttcata acggcggcat
     5761 tctgtggctg gaaacgtcat ttgcactcaa catccgcgaa cagtcgatta tgaacgtcag
     5821 cttttgtggt gataaagccg gtgcgacgct gtttccagca catatctaca ccgataacaa
     5881 cggtgaatta atgacgctga tgcaacggga actggcagac gacaaccgcc atttgcgcag
     5941 catggaagcc tttatcaatc acgtacaggg caagcccgtg atgatagccg acgccgagca
     6001 ggggtacatc atccagcaac tggtggcggc gttgtatcaa tccgcagaaa cagggacgcg
     6061 tgtggaatta tgaccaggcc agtaacgtta tcagaacccc atttcagcca gcataccctg
     6121 aacaagtatg catcgctgat ggcgcagggg aacggctatc ttgggcttcg cgccagccat
     6181 gaagaagatt acacgcgcca gacgcgaggg atgtatctgg cggggctgta tcatcgggcg
     6241 ggaaaaggtg aaatcaacga actggtgaac ctgcctgata tcttggggat ggagattgcc
     6301 ataaatggtg aggttttctc gttatcccgc gaagcctggc agcgtgaact tgactttgcc
     6361 agtggagaat tacgccgtag cgttgtctgg cgtaccagca acggcacagg ttacaccatc
     6421 gccagccgtc gctttgtttc ggcagaccaa ctgccgctca ttgtgctgga aatcactatt
     6481 acgccactgg acgccgacgc gtcagtgctg atttcaacag gtatcgacgc cacgcaaacc
     6541 aaccacggtc gccaacatct cgacgaaacc caggtgcggg tgtttggtca gcatctgatg
     6601 caggggatct acaccaccca ggatggacgc agtgatgtgg ccatcagctg ttgctgtatg
     6661 gtgagcggtg atgtgcagca atgctatacc gccaaagagc gccgtttgca gcaacatacc
     6721 agtgcgcagc ttcatgcagg cgagacagtg acgttgcaaa aactggtgtg gatcgactgg
     6781 cgggatgaca ggcaagccgt tttagacgag tggggcagcg cgtcgcttcg ccagcttgaa
     6841 atgtgcgcgc agcagagtta cgaccaactt cttgcagtat caacagaaaa ctggcgtcaa
     6901 tggtggcaga aacgtcgtat cacggtaaat ggcggcgaag cgcacgatca gcaagcgtta
     6961 gattatgcgc tttatcatct gcgcatcatg acgcctgctc acgacgagcg cagcagtatt
     7021 gcggcaaaag gcttaaccgg cgaaggctac aaaggccacg ttttctggga tacagaagta
     7081 tttttgctgc cgttccatct gtttagcgat ccgacggttg cccgaagttt actgcggtat
     7141 cgctggcaca acttgccagg cgcgcaggag aaagcgcgac gcaacggctg gcagggcgct
     7201 ctgtttccgt gggaaagcgc gcgcagcggc gaagaagaga cgccggaatt tgccgccatt
     7261 aacattcgca ccgggctgcg gcaaaaagtg gcctcggcgc aggcggaaca tcatcttgtg
     7321 gccgatatcg cctgggcggt tattcaatac tggcagacca cgggggatga aagtttcatt
     7381 gctcatgaag gcatggcgct acttatggaa actgcaaagt tctggattag ccgcgcggtg
     7441 agggttaacg accgtctgga aattcatgat gttattgggc cagacgaata taccgaacat
     7501 gtcaataata acgccttcac cagctatatg gtgtattaca acgtccagca ggcgctgagt
     7561 attgcccgcc agtttggctg tagtgacgac gcgtttatcc atcgtgccga aatgttcctt
     7621 aaagaactgc ggctgccaga aattcagccc gacggtgttt tgccgcagga tgattcgttt
     7681 atgactaagc cggcgattaa tctggcgaaa tacaaagcgg cggcggggaa gcaaaccatt
     7741 ctgctggatt attcacgcgc acaagtgaac gagatgcaga tcctcaaaca agctgatgtg
     7801 gtgatgctca attacatgct gccagagcag ttctcagcgg catcgtgtct tgccaatctg
     7861 caattttatg agccgcgcac tattcacgac tcgtcattaa gtaaagcaat ccacggcatt
     7921 gttgccgcac gctgtggcct gttggcgcaa agttatcagt tctggcgcga ggggactgaa
     7981 atcgatcttg gtgctgatcc gcatagctgt gatgacggta tccacgcagc cgcaactggc
     8041 gctatctggc tgggggcgat tcaggggttt gccggggtga gcgtgcatga cggtgaattg
     8101 catctcaatc cggcgttacc tgagcagtgg caacagttgt ctttccctct gttctggcag
     8161 ggctgcgaat tacaggtcac tctcgacgcg cagcgtattg cgattcgaac ttctgcgccc
     8221 gtttcactgc gtttgaacgg tcagcttata tccgtggctg aagaatctgt tttctgtttg
     8281 ggtgattata ttttgccctt caataggacc gctaccacgc atcaggagga tgaatgaaac
     8341 tgcaaggggt aattttcgat ctggatggtg taatcaccga taccgcgcat ctgcatttcc
     8401 aggcgtggca gcagattgcc gctgaaattg gcatcagcat tgatgcgcag tttaacgagt
     8461 ccctaaaagg gattagccgc gatgagtctc tgcggcgcat tctgcaacac gggggcaaag
     8521 agggcgactt taacccgcag gagcgggcgc aactggcgta tcgcaaaaat ctgctttacg
     8581 tccactcact gcgcgagctg acggttaatg ctgtgctgcc cggtattcgc aatttgctgg
     8641 cagagctccg tgcacagcag attccggtag ggctggcttc cgtctccctg aatgcgccga
     8701 cgattttagc ggcgctggag ctgcgcgagt ttttcacctt ctgcgcggat gcttcccagc
     8761 ttaaaaactc gaaaccggat ccggaaatct ttctcgctgc ctgtgccggg ctgggcgtgc
     8821 cgccgcaggc atgtatcggc attgaagatg cgcaggcggg cattgacgcc attaacgcca
     8881 gcggtatgcg ctcggtgggg atcggcgcgg gcttaaccgg ggcgcaatta ctgttgcctt
     8941 caacggactc tctgacctgg tcgcggttat cggccttctg gcaaaacgta tagcaaagga
     9001 atcaacatgg ctcagctttc gttacaacat attcaaaaaa tctacgataa ccaggtgcat
     9061 gtggtgaagg acttcaacct ggaaattgcc gataaagagt tcatcgtgtt tgtcggcccg
     9121 tcgggctgcg gtaagtcgac caccctgcgc atgattgccg ggcttgagga gatcagcggc
     9181 ggcgatctgt tgatcgacgg caaacgaatg aatgacgttc cagccaaagc acgcaatata
     9241 gcgatggtgt tccagaacta cgcgttgtat ccgcatatga cggtttacga caacatggcg
     9301 tttggcctga agatgcaaaa aatcgccaaa gaggtgattg atgagcgggt gaactgggcg
     9361 gcgcaaattc tcggcctgcg tgagtacctg aaacgcaagc cgggcgcgct ttccggcggg
     9421 caacgccagc gtgtggcgct cgggcgggcg attgtgcgcg aagcgggcgt gtttttaatg
     9481 gatgaaccgc tctctaacct tgatgccaag ctgcgcgtgc aaatgcgcgc agagatcagc
     9541 aagctgcacc agaagctgaa taccaccatg atctacgtga cccacgatca gaccgaagcg
     9601 atgaccatgg cgacgcggat tgtgattatg aaggatggga ttgttcagca agttggtgcg
     9661 ccgaaaacgg tttataacca accagcgaat atgtttgttg ccggatttat tggatcaccg
     9721 gcgatgaatt ttattcgtgg cacgatcgat ggcgataaat tcgttacgga aacgcttaaa
     9781 ttaaccattc ccgaagagaa attagcggtt ctgaaaacac agggaagttt gcataagccc
     9841 atcgtgatgg gaatacgtcc ggaagatatt catccggacg cgcaagagga aaataacatt
     9901 tccgccaaaa ttagcgtggc agaattaacc ggtgcggaat ttatgctcta caccacggtt
     9961 ggggggcacg agttagtggt ccgtgctggt gcgttaaatg attatcatgc aggagaaaat
    10021 atcactattc attttgatat gacgaaatgt catttctttg atgcagaaac ggaaatagca
    10081 attcgctaaa tacaggggga aggcattccc ccagaataat acaaggaaca ataatgaaaa
    10141 agttattacc ctgtaccgca ctggtgatgt gtgcgggaat ggcctgcgca caggccgagg
    10201 aaaggaacga ctggcacttt aatatcggcg cgatgtacga aatagaaaat gtcgagggtt
    10261 atggcgaaga tatggatggg ctggcggagc cttcagtcta ttttaatgcc gccaacgggc
    10321 cgtggagaat tgctctggcc tattatcagg aagggccggt agattatagc gcgggtaaac
    10381 gtggaacgtg gtttgatcgc ccggagctgg aggtgcatta tcagttcctc gaaaacgatg
    10441 atttcagttt cggcctgacc ggcggtttcc gtaattatgg ttatcactac gttgatgaac
    10501 cgggtaaaga cacggcgaat atgcagcgct ggaaaatcgc gccagactgg gatgtgaaac
    10561 tgactgacga tttacgtttc aacggttggt tgtcgatgta taaatttgcc aacgatctga
    10621 acacgaccgg ttacgctgat acccgagtcg aaacggaaac aggcctgcaa tataccttca
    10681 acgaaacggt tgccttgcga gtgaactatt atctcgagcg cggctttaat atggacgaca
    10741 gccgcaataa cggtgagttt tctacgcaag aaattcgcgc ctatttgccg ctgacgctcg
    10801 gcaaccactc ggtgacgccg tatacgcgca ttgggctgga tcgctggagt aactgggact
    10861 ggcaggatga tattgaacgt gaaggccatg attttaaccg tgtaggttta ttttacggtt
    10921 atgatttcca gaacgggctt tccgtttcgc tggaatacgc gtttgagtgg caggatcacg
    10981 acgaaggcga cagcgataaa ttccattatg caggtgtcgg cgtaaattac tcgttctgat
    11041 aatgggctaa attgccggat gcggcacgag tactttatcc gaactataaa tgtaggccgg
    11101 ataagatgcg ctagcatcgc atccggcatt caggcaaggt agctggtatt tatttcagcg
    11161 tcatatgcgt ggcaacggta atattctgtg gtgacggttt tccagaaatt aagcggaata
    11221 ataactcaca gctttgttga cctaactcct gtgttgggat atcaataccg cctggcgcag
    11281 gcgttaaaat aaacgacagc gtttcattac tataacccac caccgctaac tgctgcggaa
    11341 tagcaatatt tttctctgct gccgcacgat aaatactcat taatttcagg ctgtcagtag
    11401 caaacactgc ctcaggcaac ggtgactggc ttaataattc ccgcgcggct tgcagtgctg
    11461 tttcatgggt ataaccgccg tcaacaatcc attcatcacg cactgcaata ttatgcgcag
    11521 ccaggctctg cttataacca tttacccgat ccactgaaac atggacatca agcggagcat
    11581 gcaggcaggc aatattttga tgcccacttt caattaatgc atcggtcaac gcaatgctgt
    11641 cgccaaaatt atcggtatcg acagaataaa catgggcata ttgaccttca actttgccaa
    11701 tcaccacgac aggaatatcg tatttatcaa gttgggcaaa aaatgactca tccgctggcg
    11761 aacttagcat aataatgcct ttaatcattt tctgcttaat tttgctttca catttttgta
    11821 agtcttctgc cggattgtgc gacgtctgca atatcacgtc gaaaccttcc tcttcagctt
    11881 tggcggtgat ggcatgcaaa acttcagaga aaaacggatt acccgccgta gttttggtcg
    11941 aacgggtaga aatcaccata atggcatcaa aacccgaaga ggtcagcgcg cgggccagct
    12001 tgtttggctg atactgtaat tcttcaatgg cccgtaacac tttttcgcgc gcttccgggg
    12061 agatattagt ttgcttattc agcacgcgtg atacggtgga ttttgatacg cctgcaaccc
    12121 tggcaatatc ataaatagta ggggacatag gtcaggaact ccgtccgaac gtgaatgctg
    12181 cacatcttat ggagttcgcc cgtcgatgac aacacgggca gttgataatc aatggcctgg
    12241 cccccacatt catatcctta cgaatgattt ttttctggtc ttcagagcag cacaggacag
    12301 caatgaagcg acttaaaaat gaacttaatg cgctggtgaa tcggggtgtc gacagacatc
    12361 tgcgcctcgc cgtaaccgga ctaagccgca gcggcaaaac agcgtttatc actgcgatgg
    12421 tcaatcagtt gctcaatatt cacgccggag cacgcttgcc gctgttaagc gcggtgcgtg
    12481 aagagcgcct gctgggcgta aaacgcattc ctcagcgtga ctttggcatt ccgcgcttca
    12541 catatgatga aggactggcg cagttatatg gcgatcctcc cgcctggccg acgccaacgc
    12601 gcggcgtcag tgaaatccgc ctggcgctac gttttaaatc gaacgattcg ctgctacgcc
    12661 acttcaagga cacctccacg ctgtatctgg agattgtgga ttatcccggc gaatggttgc
    12721 tcgacctgcc gatgctggcg caggactatt taagctggtc acgccagatg acgggcttac
    12781 tcaatggtca gcgcggcgaa tggtcggtca aatggcgaat gatgtgcgaa gggctggacc
    12841 cgctagcgcc tgccgacgaa aaccggctgg cagacattgc cgccgcgtgg accgattatc
    12901 tccaccactg taaacagcag gggctgcact ttattcagcc tgggcgcttt gtcttgccgg
    12961 gagatatggc aggtgcgccc gcgctgcaat tcttcccgtg gccggatgtc gatgcctggg
    13021 gcgagtccaa actggcgcag gccgataagc acaccaatgc cggaatgctg cgcgagcggt
    13081 ttaattatta ctgcgagaag gtggtgaagg ggttctataa gaatcatttt ctgcgctttg
    13141 accgccagat tgtgctggtt gattgcctgc aacctctcaa cagtgggcca caggcattta
    13201 atgatatgcg tctggcactg acgcagctga tgcaaagttt ccactacggg cagcgtacct
    13261 tgttccggcg tttgttttcg ccggttatcg ataagctatt gtttgctgcc actaaagcgg
    13321 accatgtgac catcgatcag cacgccaata tggtttcatt actgcaacaa ctgattcagg
    13381 atgcctggca aaatgcggcg tttgaaggga ttagtatgga ttgcctgggg ctggcctcgg
    13441 tgcaggcgac caccagcggc attattgacg tcaacggcga gaaaattccg gcattgcgcg
    13501 gtaaccggct cagcgatggt gcgccactca ccgtttatcc cggtgaagtt cctgcgcgtt
    13561 tgcccggtca ggcgttctgg gacaagcaag ggttccagtt tgaagcgttt cgcccgcagg
    13621 tgatggatgt cgacaaaccg ctgccgcata ttcgtcttga tgccgcgctt gaatttttaa
    13681 taggagataa attgcgatga ccgaaccgtt aaaaccacgt attgatttcg acggtccgct
    13741 ggaggtcgat cagaatccaa aattcagggc gcagcagacc tttgacgaaa atcaggcgca
    13801 aaattttgcc ccggccacgc tcgatgaagc gcctgaagaa gaggggcaag ttgaagcggt
    13861 aatggatgca gcgttacgtc cgaaacgcag cctgtggcgc aaaatggtga tgggcgggct
    13921 ggctctgttt ggcgcaagcg ttgtcgggca gggtgtacag tggacaatga atgcctggca
    13981 aactcaggac tgggtggcgc tgggtggatg tgccgctggg gcattgatta tcggcgctgg
    14041 cgtaggttct gtggtaacag agtggcggcg cttatggcgc ttgcgacagc gcgcccatga
    14101 acgcgacgaa gcgcgcgatt tgttgcacag ccacggcacg ggcaaaggcc gcgcattttg
    14161 cgaaaaactg gcgcagcagg cgggtattga tcagtctcat ccagcgctgc aacgctggta
    14221 tgcctcaatc catgaaacgc aaaacgaccg tgaagtggtc agtttgtatg cgcatttggt
    14281 ccagccagtt ttagatgccc aggcgcggcg cgaaatcagc cgttcggcgg cggaatcaac
    14341 gttgatgatt gcggtcagcc cgctggcgtt ggtcgatatg gcgtttatcg cctggcgcaa
    14401 tctgcgttta attaatcgca tcgccacgct gtatggcatt gaactggggt attacagccg
    14461 tttgcgtctg tttaagctgg tattgctgaa tatcgctttt gccggagcca gcgaactggt
    14521 gcgcgaagtg gggatggact ggatgtcgca agatctcgct gctcgtttgt ctacccgcgc
    14581 agctcaggga attggtgctg gacttctgac ggcacgactc gggattaaag ctatggagct
    14641 ttgccgcccg ctgccgtgga ttgacgatga caaacctcgc ctcggggatt tccgtcgtca
    14701 gcttatcggt caggtgaaag aaacgctgca aaaaggcaaa acacccagcg aaaaataatg
    14761 caatatcggg tgctgaccgg atatctttac gccgaagtgc ccgtttttcc gtctttgtgt
    14821 caatgattgt tgacagaaac cttcctgcta tccaaatagt gtcatatcat catattaatt
    14881 gttctttttt caggtgaagg ttcccatgcg tctggaagtc ttttgtgaag accgactcgg
    14941 tctgacccgc gaattactcg atctactcgt gctaagaggc attgatttac gcggtattga
    15001 gattgatccc attgggcgaa tctacctcaa ttttgctgaa ctggagtttg agagtttcag
    15061 cagtctgatg gccgaaatac gccgtattgc aggtgttacc gatgtgcgta ctgtcccgtg
    15121 gatgccttcc gaacgtgagc atctggcgtt gagcgcgtta ctggaggcgt tgcccgaacc
    15181 tgtgctctct gtcgatatga aaagcaaagt ggatatggcg aacccggcga gctgtcagct
    15241 ttttgcgcaa aaattggatc gcctgcgcaa ccataccgcc gcacaattga ttaacggctt
    15301 taatttttta cgttggctgg aaagcgaacc gcaagattcc cataacgagc atgtcgttat
    15361 taatgggcag aatttcctga tggagattac gcctgtttat cttcaggatg aaaatgatca
    15421 acacgtcctg accggtgcgg tggtgatgtt gcgatcaacg attcgtatgg gccgccagtt
    15481 gcaaaatgtc gccgcccagg acgtcagcgc cttcagtcaa attgtcgccg tcagcccgaa
    15541 aatgaagcat gttgtcgaac aggcgcagaa actggcgatg ctaagcgcgc cgctgctgat
    15601 tacgggtgac acaggtacag gtaaagatct ctttgcctac gcctgccatc aggcaagccc
    15661 cagagcgggc aaaccttacc tggcgctgaa ctgtgcgtct ataccggaag atgcggtcga
    15721 gagtgaactg tttggtcatg ctccggaagg gaagaaagga ttctttgagc aggcgaacgg
    15781 tggttcggtg ctgttggatg aaatagggga aatgtcacca cggatgcagg cgaaattact
    15841 gcgtttcctt aatgatggca ctttccgtcg ggttggcgaa gaccatgagg tgcatgtcga
    15901 tgtgcgggtg atttgcgcta cgcagaagaa tctggtcgaa ctggtgcaaa aaggcatgtt
    15961 ccgtgaagat ctctattatc gtctgaacgt gttgacgctc aatctgccgc cgctacgtga
    16021 ctgtccgcag gacatcatgc cgttaactga gctgttcgtc gcccgctttg ccgacgagca
    16081 gggcgtgccg cgtccgaaac tggccgctga cctgaatact gtacttacgc gttatgcgtg
    16141 gccgggaaat gtgcggcagt taaagaacgc tatctatcgc gcactgacac aactggacgg
    16201 ttatgagctg cgtccacagg atattttgtt gccggattat gacgccgcaa cggtagccgt
    16261 gggcgaagat gcgatggaag gttcgctgga cgaaatcacc agccgttttg aacgctcggt
    16321 attaacccag ctttatcgca attatcccag cacgcgcaaa ctggcaaaac gtctcggcgt
    16381 ttcacatacc gcgattgcca ataagttgcg ggaatatggt ctgagtcaga agaagaacga
    16441 agagtaagcg cgaatatgcc tgatggtgca acaccatcag gcatattaaa ttatgctttc
    16501 agtacagcca gagctgcttc gtaatccggc tcggtggtga tttcatccac cagctggctg
    16561 aaaatcacat tgtcattttc gtcaataacc acaacggcac gcgctgccag acctttcagt
    16621 gggccatcag caattgccac accgtaggct tgcaggaatt cagcgttacg gaaagtggag
    16681 agggtgataa cgttgttcag accttctgcg ccgcagaaac gagactgggc gaacggcaga
    16741 tcggcagaaa tacacagcac aacggtgttg tcgatctcgg ttgccagttg gttaaactta
    16801 cgtactgatg cggcgcaaac tccggtatca atactcggga aaatgttcag cactttgcgt
    16861 ttacccgcaa actgaccgag ggtgacgtca gacagatctt ttgctacgag agtaaaagtc
    16921 tgcgctttgc tacccgcctg cgggatggaa ttggcgactg taaccgggtt gccctggaaa
    16981 tgaacggttt gtgacatgat tatctttcct gtttacatat agttaacgtc acacctagtt
    17041 tatgctaact gtcaataaca cagcaaacgc tatttgcgct taatccgcag accaccgcga
    17101 caacaaggag taaagatgag aaccgttaag gtattcgagg aagcctggcc cttacatacc
    17161 ccgtttgtga ttgcccgggg aagtcgcagt gaagcgcgcg tggtggtggt tgaactggaa
    17221 gaagagggta ttaaaggcac cggcgaatgc acgccgtatc cgcgttatgg ggaaagtgat
    17281 gcctcggtaa tggcgcaaat tatgagcgtc gtaccgcaat tagagaaagg gctgacacgg
    17341 gaggagttgc aaaaaattct ccctgccggc gcagcacgta atgcgctgga ttgtgcattg
    17401 tgggatctgg cggcgcgaaa acagcagcaa tcgctggctg atttgatcgg cataacgctt
    17461 cctgggacag ttatcactgc acagacggtt gtcatcggta cgcctgatca gatggccaat
    17521 agtgcatcaa cactctggca ggcaggcgcg aaattactga aagtgaagct ggataaccat
    17581 cttatcagtg agcggatggt ggcaattcgc accgctgtgc ccgatgcgac gctgatcgtt
    17641 gatgcaaatg aatcctggcg tgcagaaggg ttggcggcgc gttgccagct attggcggat
    17701 ttaggcgttg cgatgcttga acaaccgctt cctgcgcagg acgatgcggc actggagaat
    17761 tttattcatc cgttgccgat ttgtgctgat gaaagttgtc atactcgtag caatctgaag
    17821 gcgctgaaag ggcgctatga gatggttaac attaagctcg ataaaaccgg gggtctgacg
    17881 gaagcgctgg cgctggcgac tgaagcacgt gcacaaggtt ttcgtctgat gctgggctgc
    17941 atgttgtgta cctctcgtgc cattagcgcc gctttaccgc tggtgccgca ggtcagtttc
    18001 gccgatcttg acggaccgac ctggctggcg gtagatgtgg aaccggcgct tcagttcacg
    18061 acgggcgaat tgcatcttta ggatgccagc gcagcaagtt tgccatcgca aagagatatt
    18121 tttcgctggc ttcgtcggag gagatgggcg gaaactctgc ggtgatgcaa tgcaagttga
    18181 gatctgcaca ccaactgccg aaagagccag gcgtttcata accgacgctg gttaccagag
    18241 gcaattcaaa cgcctgggcc agccattcgc ctaattcgct gtgtctggga tcttcaatac
    18301 aggccagtgg atcgtggaat gaaaccaccc aggcaggttg aatgcggtgt ataagctggc
    18361 acagcgcctg ggtttcaggt tctgagccag gtttgtcacc tgtcagtaat acgacatcac
    18421 gttcttcagc ggcgctgttc cagcgataaa ccgtttcacc ttccttccag ttcgccgccg
    18481 gaaagtttct gtttaaatcc acaccattag cattggcccg taaccccagc tggcagccgt
    18541 caggattcac acacagcacc acatgatggc ggcgcaaaga aggtgtcaac gtccgcagag
    18601 cacaggagag cgtgacgacc gaagagtttt catcaccgtg agtgccagcc agaatcaaac
    18661 cactttcgcg gctggctgca ggggcaggaa accagattaa cggcgcgccc aataatgaac
    18721 gtccgtaatg ttctgttccg ggcggaaatg cgccgcgttc ggcgcgtggg cgggttacgg
    18781 tcatgatcgt ctctggttca ggaagtatta caggcagtgt tgtgcaaatt ttgcggatta
    18841 tcaaatcctt ttcttcaacg ctatgtcatc gatcttgtat cacttttttt gccggaagtt
    18901 gtttgcattt ttattgatgt gcagatatta gaattatgct gacttagatg taagtcgtaa
    18961 tgttatatac atggcgtatt ttattcttcc tggttctaca catgttaatc gcatcggatt
    19021 gatcagttca ccttaataag tttaataaat cgtactgcac atagtacatt acgatggtaa
    19081 ctatgtaatt gatttacatg aataatggct tgtcaccatc agtcacggat tgattgatac
    19141 ctatattctc accaatgtgt ttctcaggtt tatcgatgat atctgtgatt aatgctgcga
    19201 cactcttacg tgatacgatc gtacatttga atggttcatt gcgtgaggta agctcataat
    19261 caataatatc ttcatcagtc agccaggcag ggcgaagaat tgtatattcc agtccagaag
    19321 cctcaattgc atccgcagcc ctgcggaatg gttttagggg ctcaccaata actgcattat
    19381 tccattcgcc aaattttcct ggaacttcat cataaatacc gagggaaaga acaaagatca
    19441 gacgtttaac atcacaggct ttcatcgcag cgatcactga atttgcctga atatctaaat
    19501 cttcccccgt aaggttggca tacacaacgt cctgtccctg catggcctgc ttcagtgctg
    19561 catgattcag tacatctccc ataataatct tgctattagt tggatagggt ttgtggattt
    19621 ttgctggctg acgggcaaat aaagtttgtt taatggtttg tttatctgcc agttgattaa
    19681 tcacatggcg ggctatctga ccgccagcac caagaatcag tacatttttc ataatatttc
    19741 tcatgaaact tatcccagat gggtgttgaa aaaaggaata actttttcca gcgcctcctg
    19801 aactgcttcc ggtttgtcat acagttcata atgtgagaaa tcgggtaatg aaactaactg
    19861 acgatcctgc gaaaccatcg cccggccata gacttccagt ccatcacgat aagcaccgaa
    19921 ggcaccgact ttttcaccaa ctactaccat caccggctga gtcagtaaca cttcagtaaa
    19981 tgcgaaggca tcccaggcca gcgttttctg tgcatgtgag aacaacattt ttgtcgcacc
    20041 tcccggttgt tgcccgcgtg gtgttttata gtagtcagtt gcttcataaa catcgcgttc
    20101 ggtcagtcca tgggcttttg ctgcatccag gctggcggga agtaattcgt tgatctgtaa
    20161 ttctccacca cgagcctctt tggttcgttg cgacgccatt gcattcaatg cgccaatcgg
    20221 atcgtaattg ctgaaacctt cccgaaaaag acgcccgata ttaacacccg taatactcac
    20281 tactgcttta attcgttttt ctgtcagcgc ggcattaatg gcatagccac cacccccaca
    20341 cactcccagc acgccaatac gttcagcatt aacatatggc agagttactg cataatcgat
    20401 aacacggcta atatcctcta cgcgctgtgt cggatcttct atccagcgag gctccccacc
    20461 agattctccc tggaaactgg cgtcataagc gagaacgaga tagccttttt cagccaatgc
    20521 tttgccatag atattgccag acgtctgctc cttacaactg ccaaaaggat ggacgctgat
    20581 aatcgccgga tactgttttg actcatcaaa atttggtgga aactgaatat tggcagcaat
    20641 atgccaggcc atatcactat tgcggatttg aatatgtttc attacagagt ttcctcaaag
    20701 aacggagcta atactgaaat tgcttcagcc acataggctt tgccgtcata aagatccata
    20761 tggtttgcgc cttcaacaat gtgatagcgt ttatcttggc ttgatgcccg atcgtacagg
    20821 tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgcat cggctgagtc
    20881 aggtacactt ccgccatatg gtaagcatca taggtaataa tctggttaag gctgcgcaaa
    20941 gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tgtggtaata ctcccaggcc
    21001 tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg cataacggca
    21061 tattctccgc tgctaatatc gctggttcgg gcgtttgaac ccgcttcaac gtatggcagg
    21121 gcatcaatag atttcacatt gttttcccaa ccattacgga acatcgaacc aatattgacc
    21181 gcactaacgg taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt ggctgtatat
    21241 cctgcaccgg cacaaattcc catcgcacca attcgggtat tgtcgacata agaaagcgtt
    21301 gtcaggtaat caatcacggc actgatgtct tcagtacgaa tgtatgggtt ttctaactga
    21361 cgcggctcgc cgccactttc accttgataa gatgcgtcat aagcaatagt gacaaatccc
    21421 ttttccgcca gttttttggc ataggttccg gccgtttgtt ctttaacgcc cccacctggg
    21481 tgagatacca caattgcctg atactgacgg gtttcatcaa attttggagg gaaatagatc
    21541 actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt attattcatc
    21601 attccgttcc tcatggagtt gtcggttcgt tttaacggtt ggtgatatca ctatagatat
    21661 tgatcattaa gttgattaga cccaaaatca tgattagacc tatcatttaa atgattaata
    21721 gattgcatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg cgtttgtcgt
    21781 cgttgcagag gagcgtagct tcactcgtgc agcagcccgc ctgagcatgg cgcagtcagc
    21841 tttaagccag atagtgcgtc gtatagaaga acgattggga ttgcggcttc tgacgcgaac
    21901 cacgcgcagc gttgttccaa ctgaagcggg cgagcatctt ttgtctgttc ttggccctat
    21961 gttgcatgac atagattcag ccctggcatc cctgagcgag ctgcagaacc gcccatccgg
    22021 gacaatacgt attactactg tagaacatgc agcaaaaacg atattgttac cagcaatgcg
    22081 cacattcctg aaatcgcatc ctgaaattga tattcagctc accattgatt atggtttgac
    22141 cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga tggataaaga
    22201 tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct caccggatta
    22261 tttttctcgc cgaagtgttc caacgtcagt gtcacaatta atagatcatc aggcaattaa
    22321 tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg gtggacgtga
    22381 agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga tcattgatgc
    22441 tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc gggctattaa
    22501 agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg gttatcacct
    22561 gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag ataggctgaa
    22621 gtataaaggt gctgtttagc actacttgct gatacattaa tttaatttct ctcttaacgt
    22681 attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg gcgacagatg
    22741 ctgattatga taccgaaaac tggtttgaac gtgcgaagcc tgaacgtagt gttcggtgtt
    22801 ctatgtgctt taccgcattt tggcgtcgat tatttacact aa
//
LOCUS       DAZFAH010000059        22536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFAH010000059 DAZFAH010000000
VERSION     DAZFAH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.059"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            292..1191
                     /gene="yegS"
                     /locus_tag="ACHJWB_002161"
     CDS             292..1191
                     /gene="yegS"
                     /locus_tag="ACHJWB_002161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4313799.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1273..2046)
                     /locus_tag="ACHJWB_002162"
     CDS             complement(1273..2046)
                     /locus_tag="ACHJWB_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4313800.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2152..3192)
                     /gene="gatD"
                     /locus_tag="ACHJWB_002163"
     CDS             complement(2152..3192)
                     /gene="gatD"
                     /locus_tag="ACHJWB_002163"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4313801.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3240..4595)
                     /locus_tag="ACHJWB_002164"
     CDS             complement(3240..4595)
                     /locus_tag="ACHJWB_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4313802.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4599..4883)
                     /gene="gatB"
                     /locus_tag="ACHJWB_002165"
     CDS             complement(4599..4883)
                     /gene="gatB"
                     /locus_tag="ACHJWB_002165"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4313803.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4914..5366)
                     /gene="gatA"
                     /locus_tag="ACHJWB_002166"
     CDS             complement(4914..5366)
                     /gene="gatA"
                     /locus_tag="ACHJWB_002166"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4313804.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5376..6638)
                     /gene="gatZ"
                     /locus_tag="ACHJWB_002167"
     CDS             complement(5376..6638)
                     /gene="gatZ"
                     /locus_tag="ACHJWB_002167"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO4313805.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6667..7521)
                     /gene="gatY"
                     /locus_tag="ACHJWB_002168"
     CDS             complement(6667..7521)
                     /gene="gatY"
                     /locus_tag="ACHJWB_002168"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4313806.1"
                     /translation="MCVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7829..8881)
                     /gene="fbaB"
                     /locus_tag="ACHJWB_002169"
     CDS             complement(7829..8881)
                     /gene="fbaB"
                     /locus_tag="ACHJWB_002169"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4313807.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9138..10415
                     /locus_tag="ACHJWB_002170"
     CDS             9138..10415
                     /locus_tag="ACHJWB_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4313808.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVECDFPRIRVMGTIGWIASGPACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10412..11416
                     /locus_tag="ACHJWB_002171"
     CDS             10412..11416
                     /locus_tag="ACHJWB_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4313809.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCTIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11413..12378
                     /locus_tag="ACHJWB_002172"
     CDS             11413..12378
                     /locus_tag="ACHJWB_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4313810.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGAL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12352..13098)
                     /locus_tag="ACHJWB_002173"
     CDS             complement(12352..13098)
                     /locus_tag="ACHJWB_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4313811.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13150..13968)
                     /locus_tag="ACHJWB_002174"
     CDS             complement(13150..13968)
                     /locus_tag="ACHJWB_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4313812.1"
                     /translation="MQLRITSRKKFTALLCVLGLISIIAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGMLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(14033..14833)
                     /gene="thiD"
                     /locus_tag="ACHJWB_002175"
     CDS             complement(14033..14833)
                     /gene="thiD"
                     /locus_tag="ACHJWB_002175"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4313813.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14830..15618)
                     /gene="thiM"
                     /locus_tag="ACHJWB_002176"
     CDS             complement(14830..15618)
                     /gene="thiM"
                     /locus_tag="ACHJWB_002176"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4313814.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15653..15749)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15841..16113)
                     /gene="rcnR"
                     /locus_tag="ACHJWB_002177"
     CDS             complement(15841..16113)
                     /gene="rcnR"
                     /locus_tag="ACHJWB_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4313815.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQEDELKREEDLDVVLKVLDSYIK"
     gene            16234..17082
                     /gene="rcnA"
                     /locus_tag="ACHJWB_002178"
     CDS             16234..17082
                     /gene="rcnA"
                     /locus_tag="ACHJWB_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4313816.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDHGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            17301..17639
                     /gene="rcnB"
                     /locus_tag="ACHJWB_002179"
     CDS             17301..17639
                     /gene="rcnB"
                     /locus_tag="ACHJWB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4313817.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17721..18755)
                     /locus_tag="ACHJWB_002180"
     CDS             complement(17721..18755)
                     /locus_tag="ACHJWB_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO4313818.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(18771..21251)
                     /locus_tag="ACHJWB_002181"
     CDS             complement(18771..21251)
                     /locus_tag="ACHJWB_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4313819.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSNYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSYSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGANLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21267..21941)
                     /locus_tag="ACHJWB_002182"
     CDS             complement(21267..21941)
                     /locus_tag="ACHJWB_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4313820.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(22022..>22536)
                     /locus_tag="ACHJWB_002183"
     CDS             complement(22022..>22536)
                     /locus_tag="ACHJWB_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4313821.1"
                     /translation="FSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINM
                     PTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCQKEVAGVKLSLITNDKITGNDKAIAS
                     SNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVL
                     SSLVMRVAQD"
BASE COUNT         5716 a         5730 c         5172 g         5918 t
ORIGIN      
        1 taagtaggaa ttacggacat tgaggccctt cagggtaact ccatgggggc tttaatatat
       61 tatattgaag atgccactgt ttagttgaat attaggtata tgctcttttt tgaaatttat
      121 cggtggcagc cgttagtatt cgctgtcccc attgcaagct cctggtggta accactgaat
      181 cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga ctccaccttt
      241 tgttttctgc tctacactat ctacagacca atcataaagg cacatacgat catggcagaa
      301 tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc cttgcgcgaa
      361 gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa
      421 ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc
      481 ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg tgagggggat
      541 gacatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc caccagtgta
      601 gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa cgccattgcg
      661 atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac aggcggattt
      721 gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg tggcgtctct
      781 tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg tgaaatccgc
      841 ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag
      901 gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt gctgcaactg
      961 cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa atctgacgaa
     1021 gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc cccacacgaa
     1081 atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat tgaaatactt
     1141 ccggcggcgt tgcgttgtcg attaccacca gattgtccat tattgcgtta atcaaactat
     1201 tctctcttga accagggaga atccgtaatg ccggatgcgg tgtaaatacc ttatccggca
     1261 tacagaacaa tactacaccg taattaattt cacccgtgtc ttctgcaatg ccagtttatc
     1321 cacctcagac acagcatcat cggtaataat aatgtcaatt ttttcgattg gcagtacctg
     1381 attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt tattagcggc
     1441 tgtgaccatc gcaccgctaa cggaataacc ctcattaaag gtcgtaatac cattaaccgc
     1501 atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca atgaacgctc
     1561 ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac agacgaccag
     1621 agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg taatcaccgt
     1681 gatatttttg gcggtcatta atccttcggc gataagcatt gtggtacttc cgctgtcgag
     1741 aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc tgtttttggg
     1801 cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg tttcactatt
     1861 accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt tttgttcgag
     1921 aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg ccagatcctg
     1981 aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc gctcgaatga
     2041 attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg gaccattggt
     2101 gaatgatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt ttcatggaat
     2161 gagcaacact ttgcccggca tggcattacg agcgatgtca cgcaccgcct gggtgaagct
     2221 ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa
     2281 ccggctcgcc gtttcccact cctgccccgg ccaagggctg gaatagttca tccaactgcc
     2341 gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta aatgcagatc
     2401 ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct ctaccgccag
     2461 ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaaac gcagttcgcg
     2521 taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc
     2581 gaaagatttt gccagtgcca gtttttctga gctaatgtcg atagccgtca cactctttgc
     2641 tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac caataataat
     2701 aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcagaccaa cggtaatcgg
     2761 ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga cattttttcg
     2821 cttaacgaca atatattcag caaatccacc atcacgccgc gagccaataa aatcatattt
     2881 tgcgcactgg gaataaaacc ctttcagaca ctctggacaa gtaaaacagg gtaataacgg
     2941 cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca ccgcatcaat
     3001 atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat ttttaaatat
     3061 cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca cctcatcctg
     3121 atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag tatcattcac
     3181 cactgatttc ataaaaactc ctgattgtta agggggataa cctccccctc aggtaaaaat
     3241 tattctgcga gaacgacttt ctcttgttta ataaagccac gcgctctacg ccaggtcatg
     3301 aaaataccgg tcagataaat tgcaccgata ataatgaaac cgggaatatt ttgcggggag
     3361 aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga agccaccata
     3421 cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc aatagtttgc
     3481 gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt gcggaacaga
     3541 tttccacgat gcacggcgac cgccatcgcc acgaagaagc cgatggtggc aagatcgcca
     3601 aacggcagca cctgattacc cggcacacaa acagcaatta aaatggtgag tgggataaaa
     3661 atcaggcttg ccgataccac cgccgtatgt cccagcagca acgccggatc aaggccaatc
     3721 aggaactcct gaccgccgaa cttcgcctgt aaacgactac gagcctgctt agcgatgggc
     3781 gttaaaccat ccatgatggg tttaatcacc cgtggcatta gcagcatcac tgccgccgtt
     3841 tttaccgcca gctgcaatac acctttgaca tcgtaacccg cgaggatgcc gataatcagc
     3901 cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg ctgaatatcg
     3961 tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc atcgaccagc
     4021 accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc ttccagctca
     4081 aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga
     4141 attaccacac cagccatccc tatcatccat gaaccggttg ccagatgcag caacgcgccg
     4201 gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt catacgggtc
     4261 agtaacatcg ccacgttaac caatatcgca atcggaatcg ccaccagcgc aatttgcgac
     4321 gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag attcaggtcg
     4381 aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa gccaatcaca
     4441 aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca atcgcctgcc
     4501 ttcatgccta atattttaga aaaaataatg atgacaatcg gaagcatcac cgtagggccg
     4561 aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa gatagtcaga
     4621 attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc gtgaactaac
     4681 ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg cacaccatcc
     4741 atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat attatgattc
     4801 tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa
     4861 gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg gatttattcg
     4921 tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg aagagtgatt
     4981 agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat
     5041 tgctgctgcg gattttccac aatcaacgca ataaccaacg atacctccac gtcgttatca
     5101 tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat ggctgacgac
     5161 ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag cattatcccg
     5221 gtagggaatt ctgcttctct ggcaattaat gcctgtggcc aggtatcatg aaccacacct
     5281 ttggcgagca tctcattacc gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt
     5341 ccgctacgaa caaacaggtt agtcatatac cgtccttatt ccgcacagcc gtagcgatag
     5401 gcgcgcaaaa catcataaat tttatccata atcagctgat gcggtattgc tgataattcc
     5461 ccggactgaa tgcgttcaaa ttgtttggga agatactgac taatcatgcc cagtgggatg
     5521 tccacgcctt caagattcac catcatcgtt tcgacgctat ttttaatccg actatgcggc
     5581 cagtaataac gaatacgatc cgacaggctg taacgaatat ccagtaatga atcgttaaaa
     5641 cccgtacgat aatatttttt ccagtattgc ggttcgtcga gcatcacttc ttcaattacc
     5701 gccaggcaac cgctgcgatt ttcgggggcg ataagttcct gctcaatttg tgccagtgca
     5761 aatatcgcct cgcgtaaagc aaaggttaat gcgggaccga ctttcaatat tgcaaagtga
     5821 tcgcggacta attcccaata agccgtccgg gtctggtaat cggtagaatg tgcttcataa
     5881 accattcggg tgttttctat ccattgcgcc agcgcctgcg cttcctgcgg ctgataatgg
     5941 ataatattgc tgtgatcaaa ttccacaccc ggctgcacca cgatggcaat cacacgtgtt
     6001 aacgcctctg tcagcccacg ggcaataaag gccttttgat gcgtacgtaa agtattggcg
     6061 gcatcttcaa catgggtgat gtgtactgac tgaatggcgc tggcctcacc gcccggaacc
     6121 ggaacttcgg tgccaatgac atagctcagt tgctcacgct ggcaatctgt cgccacactt
     6181 tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg tttctggtgc taacggtatg
     6241 ggatcccccg cgcaggacat tgacgcatca agatgaattt tactgaagcc agcacgaaca
     6301 tatgctttta ccagctcgac ggatttttcc atcgccgcat ccgcattttc ttgctgccag
     6361 cagtttggcc ccagatgatc gccgccgaga ataatgcgtt cgcgtgcaaa cccaacttta
     6421 tcggcaatcg taaaaacaaa ttcgcgaaag tctgccggtg tcattccggt ataaccgcca
     6481 aattgattga cctggtttga cgttgcttca atcagcactt tgcgcgtgct gttgcgatca
     6541 aatgccagcg ccgcttcgat aaccaacgga tgggcagaac agactgaaca tatgccgata
     6601 tgttcaccag ctttatgccg ggcaattaac gttttcatgt tttttcctta ttaaatggca
     6661 ggtgcgttat gccctgccct cgcagccaca atcggcaatc actttgctca ccacatcgcg
     6721 cattgcggat ttagccgact gcaaataatc ccggggatcg gtcgcttcag ggtgctcggt
     6781 caggtaattt tttaacgcct gcgagaaggc atttttcagc tccgttgcaa cgttgatttt
     6841 gcatatcccc agtttgatgg tttgctgaat atccttagtc gataaccctg acgcgccatg
     6901 cagcaccagc ggtaagttca cccactggcg aatgttctcc agtctagaaa aatcaagcgc
     6961 cggtgcgctg gcatacatcc catgagccgt gccgatggcg accgccaggg aatcaattcc
     7021 ggttgcctcg gcaaattcac gcgcctgagc ggggttggta taaaaagcat cggcttcatt
     7081 gacttgcaca tcatcttcct ggccgccaag ttgccccagc tccgcttcga cgctgacatc
     7141 aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa atattttgcg caaaaggcaa
     7201 atgcgaggcg tcaatcatga ctgagcgcac gccagaacga accttctgag cgatatcgtc
     7261 aaatttcgtg tgatggtcga gatgaattgc cagtggatgg tgatattgct tcgccatcgc
     7321 gctgaccagc gccaacagat tttctgtacc agcatgagta aatgtgccag gcgttccggc
     7381 gatgatgacc ggcgcatgca ggttggcagc ggtttccacc accacttgca tcgtttcgag
     7441 attgtgaata ttgaatgccg gaaccgcata accgccgcgc tgtgcgttgt tcagcatctg
     7501 ctttgtcgat accacgcaca ttttcatatc ctgtcgtttg ttttcgattt caaaacataa
     7561 tgaaattatt tgtttttaaa tatcgagata acgatcacaa aaacgacaat atgaaaattg
     7621 ttcgaggagt gaaaggcaaa aaaacggcct cccgataggg aagccgtagc aaagtgcgcg
     7681 tgtttttatg ccggatgcgg tgtaaacata cgtggcagtg ctatgtaggc atgataagac
     7741 gcttccgcgt cgcatcaggc ataggttgcc ggatgcagtg taaacgcctt atccgtccta
     7801 cggggtggtg ttgttcgtat aaggcgtatc aggcaatagt aattttgcta tcgagataaa
     7861 cgtcctgcac ggcgttaatc agtttcacgc cgtcagccat cgatttcttg aacgccttac
     7921 gtccaagaat cagccccatt ccgccagcgc gtttgttgat aaccgcagta cgcactgcat
     7981 cactgaggtc agtttcaccg cctgcagcac cgccggagtt tatcaaccca gcccggccca
     8041 tatagcagtt agctaactga taacgcacca gatcaatcgg gttttcgctg gttaacttgc
     8101 tgtacacgcg atcgtcggta taaccgtaat taatcgcttt atagccgccg ttattttccg
     8161 ccattttttg tttgacgata tctgcgccga tggttgccgc cagatggttt gcctgaccgg
     8221 tcaggtcggc ggaaacatgg taatcaacgc catctttctt aaaggcggag ttacgcaaat
     8281 aggcccacag cacggtcacc atgcccagct catgcgcacg ttcaaaagcc gcagaaattt
     8341 cttcaatctg gcgacgtgac tcttccgagc caaaatagat agtcgcacca accgccaccg
     8401 cgcccatgtt gaaagcctgc tccacgctgg catacagcgt ttgatcgtag gtattcgggt
     8461 aacttagcgt ctcgttgtga ttaagtttga cgagaaatgg aatgcgatgc gcatagcgcc
     8521 gcgataccga cgccaacacg ccgtaagttg atgccacaca gttacagccc gcttcgatcg
     8581 ccagttcaac aatgtttttc gggtcaaagt agagcgggtt agcagcaaat gaagctccgg
     8641 cagagtgctc aacgccctgg tcaaccggca gaatagaaag atatcctgtg ccagccagac
     8701 gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc tggcgggcga ttattgtcaa
     8761 tcattacgcg gtctacgtag tcatgtccgg ggagataaag ctggtcagaa ggaattgtca
     8821 tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca atatctgtca
     8881 ttactatgct cccgtaaatt ccgattggat atcggctatg gattgtcctg gcccgccttt
     8941 tgcgggcaat cataatcctg gtcgttacgg gcgagttttt ccatcttttg gatgtttttt
     9001 cagcgttttc tgttggctcg attcatcaga aaaaatgtta gcgcggtcaa gctttcaccg
     9061 caaaggtact taaaaggtat tattaagtgg tattgtcatc gcgtacctta cattacctgt
     9121 catgaaggaa ttaaaagatg aaaacaacag caaagctgtc gttcatgatg tttgttgaat
     9181 ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa agcggtttta
     9241 gtgccggaga aattggctgg tcgtatgcct gtaccgccat tgcggcgatc ctgtcgccaa
     9301 ttctggttgg ctccatcact gaccgctttt tctcggcgca aaaagtgctg gcggtattga
     9361 tgttcgcagg cgcgctgctg atgtatttcg ctgcgcaaca gaccactttt gccgggttct
     9421 ttccgttact gctggcttac tctctaacct atatgccgac cattgcgctg actaacagca
     9481 tcgcttttgc caacgtgccg gatgtggagt gtgatttccc gcgcattcgt gtgatgggca
     9541 ctatcggctg gattgcctcc ggtccggcat gtggtttctt gccgcaaata ctggggtatg
     9601 ccgatatctc accgactaac atcccgctgc tgattaccgc cggaagttct gctctgctcg
     9661 gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag caccggcaaa atggatatta
     9721 aagtcatgct cggcctggat gcgctgatcc tgctgcgcga taaaaacttc ctcgtctttt
     9781 tcttctgttc attcctgttt gcgatgccac tggcgttcta ttacatcttt gccaacggtt
     9841 atctgaccga agttggcatg aaaaacgcca ccggctggat gacgctcggc cagttctctg
     9901 aaatattctt tatgctggca ttaccgtttt tcactaaacg ctttggtatc aaaaaggtat
     9961 tattgcttgg tctggtcacc gctgcgatcc gctatggctt ctttatttac ggtagtgcgg
    10021 atgaatattt cacctacgcg ttactgttcc tcggtatttt gcttcacggc gtaagttacg
    10081 atttttacta cgttaccgct tacatctatg tcgataaaaa agcccccgtg catatgcgta
    10141 ccgccgcgca ggggctgatc acgctctgct gccagggctt cggcagtttg ctcggctatc
    10201 gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatca ggaaccggta aacggactga
    10261 ctttcaactg gtccgggatg tggacttttg gcgcggtgat gattgccatt atcgccgtgc
    10321 tgttcatgat ttttttccgc gaatccgaca acgaaattac ggctatcaag gtcgatgatc
    10381 gcgatattgc gttgacacaa ggggaagtta aatgaaaaca gaacgtattc tcggtgctct
    10441 ttatgggcag gcgttagggg atgcgatggg gatgccctcc gagctttggc cacgcagccg
    10501 cgttaaagca cactttggct ggattgaccg ttttcttcct ggaccaaagg agaataacgc
    10561 ggcctgttat tttaaccgcg ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc
    10621 ggatgcgtta ctggaacgtg aaggcaagat cgatccgaat ctgattgggc gtaatattct
    10681 cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta cttggtccga cctcgaagat
    10741 tgcgcttaac gccattcgcg acggtaaacc cattgctgaa ctggaaaaca acggcgtgac
    10801 caacggcgca gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc acgatgttga
    10861 ttcctttatt gatgatgtgg cgctggcgtc cagcccgacc cataaatccg atctggcggt
    10921 tgcaggcgcg gtagtcatcg catgggcgat ttctcgtgcc attgacggag aaagctggtc
    10981 agcgattgta gattcactgc cttcaattgc gcgacatgcc cagcaaaaac gcatcactac
    11041 cttcagcgcc tcactggcag cacgtctgga gattgcgctg aaaattgtgc gcaatgccga
    11101 cggcaccgaa tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta ccagcactat
    11161 tgagtccgtt ccgtgcacca ttgcgctggt tgaactggca caaaccgacc cgaatcgctg
    11221 cgccgtcctg tgcgctaacc ttggcggcga cacagacacc atcggcgcta tggcgacggc
    11281 gatttgcggc gcgttgcatg gcgttaacgc tatcgatcct gcattaaagg cggaactgga
    11341 tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca
    11401 acgggaggcg gtatgagcgg cgctcgatta cacacgctgc tgcctgaatt aacctcgcgt
    11461 cagccggtga tggtcgtcgg cgcggcggtc attgatgtga tcgccgacgc ttatgccctc
    11521 ccctggcgtg ggtgcgatat cgaactgaag cagcagagcg ttaacgttgg cggctgcgca
    11581 ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc cttgccgctc
    11641 ggtcagggcg tgtgggcgga gattattcgc aaccggatgg cgaaagaggg gttaatcagc
    11701 ctgatcgata acgccgaagg cgataacggc tggtgtctgg cgctggttga gccggatggc
    11761 gaacgcactt ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg
    11821 cgattaaccg ttgcgcctgg cagcctgctc tatttttccg gttatcaact ggcctcgccc
    11881 tgcggcgaat tgttagtgga atggctggaa aagctgcaag acgtgacgcc gtttatcgat
    11941 tttggcccac gtatcggcga tatcccggac gcattactgg cgcggatcat ggcctgtcga
    12001 cctttagtat cactcaatcg tcaagaggct gagattgccg ccgaacgttt tgctttatcc
    12061 gcagagataa caacacttgg caagcaatgg caggagaaat ttgccgcgcc gttgatcgtt
    12121 cgtctcgata aagagggtgc atggtatttc agcaacgacg cttctggctg cattccggca
    12181 tttccaacgc aagttgtaga caccattggg gcgggcgaca gtcatgcagg tggcgcactt
    12241 gccgggctgg cctctggtct gccactggcg gatgccgtat tactgggcaa tgcagtggcg
    12301 tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga actactcctc
    12361 gcacacaaaa acgtatagat cgctgcgaca gtggctaatg ctgtactcaa taggccgttg
    12421 ctgttggtca agcgccactt gcttgatcac cagcaccggt attttgctat ctaactgaat
    12481 atgtgactga aactcggcat ccggcatccg ggcgctaacg cgggaacgtg tacgttgtgg
    12541 gtaaatgtgc tggctgcgga aatagtcata aagtgaaatc ccgatggcat caacatcatg
    12601 aattaaatgc gccggaaccc acgattcctc aatcgatacc gcttcttcat cgacatagcg
    12661 aatacgctta agcaaaaaga catcacttcc tgcttcgacc gccagctgct gcgcgacttc
    12721 ttcgggacat ttcacgacac gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa
    12781 taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat
    12841 ctgcgtgccg taaccgcgcg aacgcgtcac cacaccctct tcttccagcg cctgcatcgc
    12901 cttgcgcacc gtaatgcgcg aaacgccggt taactgactt aaatcacgct caccgggcaa
    12961 aatattgcca tgctccagca ccccgctgcg cacggcattt tttaccgttt cggcaaactt
    13021 aatataaagc ggcgtgttat ccgcagctaa aatacgctcg ttcagttggg caatcagctg
    13081 ggtatgcgct tgttccattt gtctttttcc tgccagggag tctggtggga gtatatcact
    13141 gcttcttatt taaggcgttt ctttaatccc atcaacgaat ccctgcaact cctccaccgt
    13201 gccattaaac acattaaaat ccaccggacc attgatgcca tctacctgtc cacggtcgga
    13261 atgctgccag aagcgccagg ccattccgtc attgtccgga cgacgttgat aatagtgagc
    13321 cacccaccac ggatattcat taaaatagcc cgccagattc gtgtgataaa aaacggctcc
    13381 tgagtaaata atcggctttt ttcccgtgcg tttttctacc atttttagcc actgacttac
    13441 ccgcttgcgt aattcttttg ccgataattt tccccgttct tctacgtcca gcacggcagg
    13501 gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgcgcctgaa ctgaagcgga
    13561 taccgacggg gagaaataat gatacgcccc acgcagcatg ccattttcgc ggcttagttg
    13621 ccagttacgc gaaaaatagg ggtccaccag cttttcgcct tccgtcgcct taataaaagc
    13681 aaactgtaag cggataccgt tgtcgcgcat ttttgccacc cgctgccagt cgatccgctc
    13741 ctgccagcgc gaaacatcta ttccgtgaat ggtataactg gcaggaatgc gaattgcgaa
    13801 agatttaacc gggcgataac cgtagaagtg gatgtagtct ttaatctgaa ctgctgtcga
    13861 atagaaaaaa ttcaccgtct gacgcggata aattgcaatg atggaaatta gcccaagaac
    13921 gcataataaa gcggtgaatt tttttcgact ggtgattctc agctgcattg gtttcatccc
    13981 tgaatgtcag tgccaaagac tgacaataac caaagcagct atagtacggt gctcaccacc
    14041 aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg
    14101 ctaacgccga tgagagccag ctttttgcct cctgtacggt gtcagcccag tttgtatggc
    14161 gcgggcgtag tgcagccaac gccgcagaga gtgtacaacc agtgccgtgg gtgtttttgg
    14221 tcataatgcg cggtgcggta aaccgttgtt caccctcgcg agtaaacagc cagtccgggc
    14281 tttgctcatc atccagatga ccacctttca ttagcactgc gccacagccc atcgccaaca
    14341 gcgatcgccc ttgttccagc atttcctgtt cgttgcgcgc gtgtggcgcg tcgagcaagg
    14401 cggcagcttc gggcaagttt ggcgttatta atgaaacctg tggcaataat cgactgcgca
    14461 gcgtagcaac cgccgaaggt gaaagcagcg gatcgccgct ttttgccagc ataacagtgt
    14521 cgagtaccac gttttggatc tgataacgtt gcaaccgttc tgccaccgct tcaacaatat
    14581 cggtttccgc caacataccg attttagtgg tatcgattcg cacatcgctg aacaccgaat
    14641 cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac cgactgtacg ccacgggtat
    14701 tttgcgccac cagtgcagta ataactgagc aaccataagc gccaagtgcc gagaaggttt
    14761 taagatcggc ctgaatcccc gcaccaccac tcggatcagt accggcaatc gtcagagcgt
    14821 taattcgttt catgcctgca cctcctgcgt caattgccag agcgcatcaa ggaaatgtgg
    14881 aacaaaactg cctggcccct cgcttctggc gacagcgcgt tctccggctt gtttcatcca
    14941 gtgacaggca gatgcgacat tttccagcat atcgcctggt aaggcacaac aggcagcgac
    15001 aaccgccgat aatgcacagc cagttcctac cactttggtc attaacggat cgccaccgtg
    15061 aataccaatg atacgatgtc catcggtaac ataatccacc tcgccagtga ccacgacgat
    15121 tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc gcgttagctg cggcgtcagt
    15181 ggtatccact ccccgtccgc cattagcaac gccagctaat gccatgattt ccgaagcatt
    15241 accacgtatc gctgccggtt taaaagataa aagttcatga caaaaatggc ggcgataatc
    15301 gagcgcacca accgctactg gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc
    15361 aacggcagca cgcatcgcct gtgcgcgtgg ctgcgtcagt gtgccaacgt taatcaacaa
    15421 ggcactggcg atagccgcaa actgactggc ctcttcggtt tcgataacca tcgctggcga
    15481 tgcaccgagc accagcaagg tattggcagt aaaggtttgc accacatcgt tggtcatgca
    15541 gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgcg tgcgcagatt gcgctgaact
    15601 cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat gacctggtgg
    15661 tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt tctcagcctt
    15721 cacgcagaag ggcaccccga gtcgtttggt tgcgatgata aatcgcagag gaggatggta
    15781 atgtccagcg cacccgttgt aaacgaggaa aaacggcagt tttacaatag cgttaataaa
    15841 ttatttgata tatgaatcca gcaccttcag aacgacatcc agatcttctt cacgttttag
    15901 ctcatcctcc tggtgaacga tgtgttccgt cagatgacct ttaatcactt cccgcatcag
    15961 accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgcagcgc attcgtgcgg
    16021 ctcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg cacgcgcttt
    16081 cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac aagaatatga
    16141 aaaatcatag cactattaat ctactggggg gtagtatcag gtactggggg ggagtagaat
    16201 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag
    16261 caaggaaacg cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg
    16321 gaaccagggc actcaaaaac gatgatggca gcgtttatca tcgccatcaa aggcaccatt
    16381 aaacaggcgg tgatgctcgg actggcagca accatttcgc ataccgcagt ggtctggttg
    16441 attgcctttg gcgggatggt gatcagcaag cgctttactg ctcaatcagc agaaccgtgg
    16501 ctccagctga tttccgcagt gatcattatt ggcaccgcat tctggatgtt ctggcgtacc
    16561 tggcgcggcg aacgcaactg gctggagaat atgcacgggc atgattatga gcatcatcac
    16621 catcatcacg atcacgaaca tcatcaagac cacgaacatc accacgacca tggacatcat
    16681 caccatcacg aacatggcga gtatcaggat gcccatgcac gagcccatgc caatgatatt
    16741 aaacgacgct ttgatggtag agaggtcacc aactggcaaa ttttgttatt tggcttaaca
    16801 ggtggtctta tcccctgtcc ggcagcaatt accgtgctgt tgatttgcat tcagttgaaa
    16861 gccctgacac tgggcgcaac actggtcgtc agtttcagca ttggcctggc gttaacgctg
    16921 gtcaccgtag gcgttggcgc agcaatcagc gttcagcagg tcgcaaaacg ctggagcgga
    16981 tttaacactc tcgctaaacg cgccccctat ttttccagtc tgttgattgg cttagttggt
    17041 gtgtatatgg gcgtacatgg cttcatgggc ataatgcgat aactcaatcc tttaggcttg
    17101 catgttatct tcagcccagg atgattctta aatcagctat ttccgctgac tctctgctcg
    17161 ctaaggatgc ttttagggca ttctttcatt tacacttttt acgaaatcat gggatcacta
    17221 acaaaatatc gcttgtcagt tatattgtat ggcaggaaag atatgcgact gatattacag
    17281 atccccaaag tggagagttt atgaccatta aaaataagat gttgctgggt gcgcttttgc
    17341 tggttaccag tgccgcctgg gccgcaccag ccaccgcggg ttcgaccaat acctcgggaa
    17401 tttctaagta tgagttaagc agtttcattg ctgactttaa gcatttcaaa ccaggcgata
    17461 ccgtcccaga gatgtaccgc accgatgagt acaacattaa gcagtggcag ttacgtaacc
    17521 tgcccgcgcc tgatgccggg acgcactgga cctatatggg tggcgcgtac gtgttgatca
    17581 gcgacaccga cggtaaaatc attaaagcct acgacggtga gattttttat catcgctaaa
    17641 aaaagccccc tcatcatgag ggggaaatgc agacaccttg ttatttttta ttattagcca
    17701 cttgctcgtc ttgcttgtta ttagtcgtat ttcacgttga ttaatgcggt tgcctccagt
    17761 gcgccagatt taattttatt tgtatcgtag acgtagtaac tggcagttat cggaattgaa
    17821 accgttgtat ctgtaagttt tgacttcgcc gcaacttcct ttaaatttgt atatccttta
    17881 ctgctgttag tgatatttaa aagtgtgact ggcgaaccat tactttcaag aataaatcct
    17941 acgccattat cttccgcacc tttcagtatt gtgttgctgt tggttaacaa gtcagaactt
    18001 gataaataca cctgcatttt ccgtgatgtt tgattttgtt gacctaataa atttttacaa
    18061 cgcagaataa tatccccttg tttactattt attgttgctt tgttacctgt ggctggtaat
    18121 tgtgaaacgg gaatactgtc taaagtaatt aataaatcgt ccggtttgca ggttgtggtt
    18181 atcgggttgt atgacaatgt cagattaaag gaatagacac ctgcattttg tgccaatgca
    18241 tttctgtagc tatttacaca tgcatcccat gacttgccac ctaataaaca cagtagaaat
    18301 tgtactgcgg cactcccgcg tgtcgatgat gaagcactgg ataatgtcgc aacaccatta
    18361 atcatcactg aaccgccgcc ccccgcagaa acattcactt ttgcattact ggccgcaggt
    18421 gtcaccgtaa ctgaataacc actggtgaaa ctggtctgat tatttgacaa cgtgatgttg
    18481 tttttattgg tccattcaaa atgtaaattt aattttatgg tatcgttata aagtccaaca
    18541 ataatatccg acgggctcat aggggtgata gtatttgtac ttacgcatga cgaatcatta
    18601 aaattatgct catatgtcgc catttgtgtt tgagtaagtg caaaatcccc ctgaacaacc
    18661 aagttgtgct gaacagataa ctcactataa catgccgcat agctcacagg cataaccatc
    18721 atcattaata taaatagcat tatgcgaatt tccatagaac cacctgacta ttactggcaa
    18781 atataatttc tactttcatc aatctcttta ccgaaggtga ttgtgcatga aagtccttgt
    18841 tgcttatcaa ttgccacatt aaccgatggc ggtacttcat tagtgcgaat aaataactgg
    18901 cttccctggc cgacaacgcc aatattatga ccatggatat cattgacttc ataaccaaac
    18961 attaatggtt gaccatccgc tcttaacgct ttaataaacc agggcttgcg ctgatcggta
    19021 tcaaaattaa ccagtacaac cgcgccgcga taaggggcgg caattttccg gttgccacgt
    19081 aattctgctt cgctatcgct ttgcgacaca tccagcatca ggtgattttc ccgataaggt
    19141 gtcattccgt cgtatatcac cactccatta cggtttgttg tgcgatattt ttgcccattg
    19201 acataagcat ctttaattcc tggcgcattc atcacagcaa acgtttcgga aagacggttc
    19261 gccagattaa cgccacccga ccaggcgaca atgccccctg aaacactggc tccagcctgt
    19321 cgataagtac tcgactgact ataactgcca ttcactgtcg caaccggcgc gttccaggtc
    19381 aaattcgccc cagctgtcgt ttcatttccc tgatgctgat aactcaggtt ggccccatag
    19441 ttaaactgat cccggcttcc aacggttcct gataatcccg tattatttga ggcaaacccc
    19501 tgatcatcaa acgtcgttga gttagacata tatatttgcc gacgaggcgt cgtaacgtca
    19561 tcaccccaat caaagggaat cgatataaaa atattaaaac gtttctcttc atgatgattc
    19621 tcgtcatacg cctggcttgc cgcgagggta tagcttatcc gccgccagtt attggaataa
    19681 ctcaactgat aatccttact gctgccgcta cgcccccagt aatctcgcca taacgtactt
    19741 aaggacacag acccccagcc ttctggcaat gactggctca tattggcaga aaagctattt
    19801 ttacggccaa aatcgttctg gtaataatcg gcaatgtcat agatatcgtt ttcatcacgg
    19861 cgataattat ctttattgtt tgcccaaacg tgatcattaa atgtccggta atcacgcgat
    19921 gaataacgcc aggccgcaag accaaaacgc gtcgacgttt ggctcacaaa tttgttgtag
    19981 gcaatttgat aactttgccc gtcaaacaca tcgccgttgt cttgtttact atacgacttc
    20041 gtggcatcga cggaaatggc accaatgcgt gtattccagc cagtccccaa agtaaacgcg
    20101 taataattat tcgcgaccat cgagccacca tacagcgtca ataaattatt aaaaccatac
    20161 tgataacccg cctggacaaa atcactttgt ttgctcgccc cttcaatatg gctacgaccc
    20221 gccgcaaaat cataattcga cacgccgggt tgcagcatat ttggcaccgc tgcataaggc
    20281 accagatagg tggttaccga gccgtccgcc tctttcacgc tgacatcaag atctgctcca
    20341 ccaccggcca actgcaaatc tgtaatcgca aacgggccag gaggaacctc tttctgataa
    20401 accacaaaac cattctgttc aatagttacc agcgcgttac tctgagcaat cccctgcacc
    20461 cgtggcgtaa aattttgttt cgagttaggc aacatctgca tatcacgaaa caaccgcaca
    20521 ccgctgaagc gaacagaatc aaaaatatcg cttgatgtgt acatatcacc cacgcgaagc
    20581 gtgccgagaa gttgggcaaa tccacgttcc agatacaggg tattgctttt ccacacccct
    20641 ggattgttat ttgttttact gaaactggca tcagaatgca gttgccactc cagtaaattt
    20701 aacccgctgt taaaacgtac atatgtactc ttgttgttac ccgacgcttt atagtcgctg
    20761 taatactgac tcacataata agaggtataa aacgcattaa taccccgctc ccagttttcc
    20821 ggtggaacat agccactttc cagttcttcc acccaagcct gcgggacact gaaatcgaga
    20881 cgaaaaaccc cgatatccca gctatagctc ccaccctgaa caagttgctc aaatgttaaa
    20941 cattgcttac cgctggcgaa gttatcgcta ttaatgccta accgcttgat aacttctctt
    21001 gataaacatg tttcttgcgg gttgtcttta acaataatct catatttccc gcgccattgc
    21061 ttattgacat aaatatcgat gtcatactgc cccggtaagg gttgattatc atcaagacga
    21121 atatttgcaa cctgctggtc tttcattcca cctatcataa aatgggtatc aaaggtttct
    21181 tcagctgcat aagcttcaat gccgagcaat aacgctacga ttgctgatgc aagtggggtc
    21241 attctcaaca taggcagctc ctgcaattaa attttgtcac taatatagtt gccgtggtca
    21301 tcgataatgg tcagatacca gttatttgca ttattacttt tgacattaac actctgactt
    21361 tctaaggggg caatcattat agtttcataa ttgactttga cattattagc tttgacatcc
    21421 gaaatcgtca cccaattagc tgagtcattt tttatcacca atccattgcc actgcgattt
    21481 accagtaact ttttaaaggt cgctttattt accggagcaa tacccgccgg ccggtaaaac
    21541 aatttgattc tgttttgcat cgcaaacttc agtgcattct taccttcttg ttctggacta
    21601 tttggtggaa tatccagaac attcagataa aaaatgcttt ctttattagt gggcagttta
    21661 ttcggcataa ttttgatttt tacttgctgc ccggaatttg cccctatttt tgccactggt
    21721 ggcgttaaca tgaaaggaac ctgaattttt tctggtggta acgacgtatc gccatcatca
    21781 atccacgcct gcagcagcga agaacgattt ccctggttca tcaactgcac cataacttct
    21841 ttattttctg ccgggtaaat aatgcgcgtc ccgtagataa caattccggc atgtgcagga
    21901 aggaccatag aaaaaatgag taaagataat aatcctttca tcttaatgcc tcttaaatta
    21961 aaaagccgcc atggggatag cggccatgtt tattacctgg agaaacttgc aattttattt
    22021 attaatcctg cgccacacgc ataaccagag aagataacac atcacctgat tgcactgagt
    22081 tatctgtcag tttcaggtat tttgctttaa acggaatagc atattgacct tccgctgttt
    22141 tataactctc aatgttaaaa ggtgcagaaa catccaggac atcgctgtta tcacctaaat
    22201 agagatagta acccacggta tcgttgctac tggctatcgc cttatcgtta ccggttattt
    22261 tatcgttggt aatcaacgag agttttacac cagcaacttc tttctggcag ctaattttta
    22321 acatgctgct ggttacaccg actgcatcat cataggtaga atatttagct ttaccttcaa
    22381 agacagtggt tggtacagtc ggcatattaa tacttactga atcaccaccc gcttcgaatt
    22441 tacacggaga ttctgcaata ttccccttaa tagttaatgt tccggtatca acgtctgcag
    22501 caaatactcc agcgctcata aaaaaagaag aaaaga
//
LOCUS       DAZFAH010000060        22434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFAH010000060 DAZFAH010000000
VERSION     DAZFAH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.060"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(380..1471)
                     /gene="ychF"
                     /locus_tag="ACHJWB_002184"
     CDS             complement(380..1471)
                     /gene="ychF"
                     /locus_tag="ACHJWB_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4313822.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1588..2172)
                     /gene="pth"
                     /locus_tag="ACHJWB_002185"
     CDS             complement(1588..2172)
                     /gene="pth"
                     /locus_tag="ACHJWB_002185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4313823.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPCDKNKVVSFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2450..2728
                     /gene="ychH"
                     /locus_tag="ACHJWB_002186"
     CDS             2450..2728
                     /gene="ychH"
                     /locus_tag="ACHJWB_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4313824.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2783..4462)
                     /gene="dauA"
                     /locus_tag="ACHJWB_002187"
     CDS             complement(2783..4462)
                     /gene="dauA"
                     /locus_tag="ACHJWB_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4313825.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4587..5534)
                     /gene="prs"
                     /locus_tag="ACHJWB_002188"
     CDS             complement(4587..5534)
                     /gene="prs"
                     /locus_tag="ACHJWB_002188"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4313826.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5685..6536)
                     /gene="ispE"
                     /locus_tag="ACHJWB_002189"
     CDS             complement(5685..6536)
                     /gene="ispE"
                     /locus_tag="ACHJWB_002189"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4313827.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6536..7159)
                     /gene="lolB"
                     /locus_tag="ACHJWB_002190"
     CDS             complement(6536..7159)
                     /gene="lolB"
                     /locus_tag="ACHJWB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4313828.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7373..8629
                     /gene="hemA"
                     /locus_tag="ACHJWB_002191"
     CDS             7373..8629
                     /gene="hemA"
                     /locus_tag="ACHJWB_002191"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4313829.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8671..9753
                     /gene="prfA"
                     /locus_tag="ACHJWB_002192"
     CDS             8671..9753
                     /gene="prfA"
                     /locus_tag="ACHJWB_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4313830.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9753..10586
                     /gene="prmC"
                     /locus_tag="ACHJWB_002193"
     CDS             9753..10586
                     /gene="prmC"
                     /locus_tag="ACHJWB_002193"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4313831.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLLLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10583..10975
                     /gene="sirB2"
                     /locus_tag="ACHJWB_002194"
     CDS             10583..10975
                     /gene="sirB2"
                     /locus_tag="ACHJWB_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4313832.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10979..11788
                     /gene="sirB1"
                     /locus_tag="ACHJWB_002195"
     CDS             10979..11788
                     /gene="sirB1"
                     /locus_tag="ACHJWB_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4313833.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11824..12678
                     /gene="kdsA"
                     /locus_tag="ACHJWB_002196"
     CDS             11824..12678
                     /gene="kdsA"
                     /locus_tag="ACHJWB_002196"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4313834.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12827..12934)
                     /locus_tag="ACHJWB_002197"
     CDS             complement(12827..12934)
                     /locus_tag="ACHJWB_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4313835.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13362..13469)
                     /locus_tag="ACHJWB_002198"
     CDS             complement(13362..13469)
                     /locus_tag="ACHJWB_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4313836.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13874..14974)
                     /gene="chaA"
                     /locus_tag="ACHJWB_002199"
     CDS             complement(13874..14974)
                     /gene="chaA"
                     /locus_tag="ACHJWB_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4313837.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15244..15474
                     /gene="chaB"
                     /locus_tag="ACHJWB_002200"
     CDS             15244..15474
                     /gene="chaB"
                     /locus_tag="ACHJWB_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4313838.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15632..16327
                     /gene="chaC"
                     /locus_tag="ACHJWB_002201"
     CDS             15632..16327
                     /gene="chaC"
                     /locus_tag="ACHJWB_002201"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4313839.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(16371..16724)
                     /gene="ychN"
                     /locus_tag="ACHJWB_002202"
     CDS             complement(16371..16724)
                     /gene="ychN"
                     /locus_tag="ACHJWB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4313840.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16909..18303
                     /gene="ychO"
                     /locus_tag="ACHJWB_002203"
                     /gene_synonym="iatA"
     CDS             16909..18303
                     /gene="ychO"
                     /locus_tag="ACHJWB_002203"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4313841.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSHGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18304..18954)
                     /gene="narL"
                     /locus_tag="ACHJWB_002204"
     CDS             complement(18304..18954)
                     /gene="narL"
                     /locus_tag="ACHJWB_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4313842.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18947..20743)
                     /gene="narX"
                     /locus_tag="ACHJWB_002205"
     CDS             complement(18947..20743)
                     /gene="narX"
                     /locus_tag="ACHJWB_002205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4313843.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21082..>22434
                     /gene="narK"
                     /locus_tag="ACHJWB_002206"
     CDS             21082..>22434
                     /gene="narK"
                     /locus_tag="ACHJWB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4313844.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         5533 a         5597 c         5791 g         5513 t
ORIGIN      
        1 aataagaaaa agcaaaaaac atcgatattc atttttggtc aatgggtttt ctggcctatc
       61 gttgttattg tgctactggt tatcattttc atcttgccag catattggag cgtgatcaat
      121 tttgatcagc tgtgaaggtg gctgatgtgt agtgatacaa aaacttacgt tatatcactg
      181 ctaatgagat atccgtcatc tgaacactta tgtctttaat tattctcgtg gttcactata
      241 ggcaataagc acaaaagtgt aggatgttac aagaatgatt aggactcggt gaaatgaaaa
      301 atccacgcaa ttgcgtggat tttatatact tttgcgctct tcatgagatt tagcgaaacc
      361 tcatgagaca ataaattaat tagacgttga aaaggaagtt catcacatcg ccatctttca
      421 cgatgtaatc tttaccttct gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt
      481 tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg gataaagcct ttttcaaaat
      541 cagtatggat tttgcccgct gcctgcggcg cggttgctcc aaccggaatg gtccatgcac
      601 gcacttcttt caccccagcg gtgaagtaag tttgcaggtt cagcagctta taaccggcac
      661 ggatcacacg gttcaggccc ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt
      721 cttcgtcgtc cagttcggca atgtctgctt caacagcagc acaaaccgga accacaacag
      781 aaccttcttt cgccgcgatt tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt
      841 cttcgttgac gttggcgatg tacattgttg gtttcagcgt caggaagctc aggtaacgaa
      901 tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag catacctgca ttttccaact
      961 ggggcaggca tttttccagg accgccagct cagctttcgc gtctttatcg ccacctttgg
     1021 ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt
     1081 cggtgttgat aacttcaata tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt
     1141 tgtcattttc aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg atgttggtca
     1201 ggaactggtt acccagacct tcgcctttcg atgcgccttt taccagaccg gcgatatcga
     1261 caaattccat ggtcgtggga agcgtacgct gcggttttac gatttcagcc agttgatcca
     1321 ggcgaggatc aggcattggt acgacgcctg tgttcggctc aatggtgcag aatggaaagt
     1381 tggccgcttc aataccggct ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg
     1441 gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata tcttaataat
     1501 caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt atacacggca
     1561 ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca atcggttcgt
     1621 tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt cgtcaatggc
     1681 ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac tgacaacttt
     1741 atttttatcg cacggatgac cgattccgat gcgtaaacgg tgaaagttag ggttattacc
     1801 caatttactg atgatgtctt tcagtccatt gtgaccacca tggccaccgc ccaatttaaa
     1861 tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt cgtccggatt
     1921 aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca taaatgtagt
     1981 cgggactaac aggcggacat cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa
     2041 tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc
     2101 accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa
     2161 tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt
     2221 gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcctgagt
     2281 ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg
     2341 gtgtaattgt gatcacgccc gcacataacc cacagggtgt tgtctatact ttacacataa
     2401 ggaagagggg gattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa
     2461 aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg
     2521 cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca
     2581 tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg
     2641 cgggcatgaa caggtgtgcg accgttactg gtgggttcgt cactatgaca aacgttgccg
     2701 ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg
     2761 cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga ttcgggaaga
     2821 acgcgaggcg tcccgggatc ggttgaatgc ctgcgcgagc catagtgcgc agtggctgga
     2881 attccacgtt gcacacgcgc agttcacatc cttcgggcag acgcttcaca aaacgctgga
     2941 acgcatcaag accaccagca tcaagtaccg gaacggcatc ccacttcaga atcacaatcc
     3001 gtttgccttc aagacgtgac tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca
     3061 gcgggccaat aacgcgcagt accagaacat cgtctggaac atctacgact accggtgcca
     3121 ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc
     3181 tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc atgacaatga
     3241 tgtcatcttt cggtgcatga cgcagcaagt cgaccacttt atgcgcttca ctcatgttcc
     3301 acgccaccat caacagcagg gctgccatag cggaaagcgg cagccaggag agcagcggtg
     3361 ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgctgag ataggggaag
     3421 ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg gtaataccac
     3481 caaagaacgg agcgataata ttccccagtc cctgtccaac cagttcgctg ttcgccttgt
     3541 gtttcgtccc ggtcatacca tccagtacca cggcgcagag cagagattcg attgcgccga
     3601 gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag gttagcgtga
     3661 attctgaatt aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt
     3721 taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc
     3781 cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac
     3841 cgggtaaacg aatgcccaga cgcggccaaa aaacaagaat acctagcgtc acaatgccaa
     3901 tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta
     3961 gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac
     4021 cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa
     4081 tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa gatccccgac agcaaggtcg
     4141 caaccagcag tcctgccagt ccaaactgtt gcgacacggg atagagaatt accacaaatg
     4201 ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg acaatccccg
     4261 caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata gccaacgcca
     4321 tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc
     4381 gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat
     4441 gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta
     4501 aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc
     4561 gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagagat cgattcttcg
     4621 ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt acgcacgttc
     4681 ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac ttcatcaatt
     4741 acagagttac gcaggttgtt cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca
     4801 aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag cgtaccgcca
     4861 gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc gatgatatgc
     4921 atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat atcggtatcg
     4981 ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca
     5041 attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct accaaatacg
     5101 ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc cactgtcagc
     5161 acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt gattggtaca
     5221 cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat aacagcggtg
     5281 atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc catcaggttg
     5341 tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg tacattttca
     5401 ttaatttgta cgctgacttc gccatcgcta aagcgaccta cagcggcgtc gccgagtgaa
     5461 gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc agcaaaaagc
     5521 ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag aatctgccga
     5581 aaactgtgcg ggccaggcat gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg
     5641 tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag catggctctg
     5701 tgcaatgggg aaagattaac gcctttcgcc acaaagccat tgagccattc cggggcttgc
     5761 tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca ggcccctgtc
     5821 ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc aacctcgcga
     5881 aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag caacgtttct
     5941 attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac cggagtcgga
     6001 atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac cggcgttagt
     6061 atttcaccaa cgccttcggc aaacgcggca tgcccccgaa caaagacagg aacatctgcg
     6121 cccagcgtca gccccatttc cgccagctca tccatgctta gcccgcattg ccagagatga
     6181 tttaatgcca ccaggaccgt cgcggcattg gatgaaccac cgccgagacc gccgcccatc
     6241 ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcggaagacg cccgctgtct
     6301 gccgcagttt tcatcaacaa ccgcgctgcg cgaacgatca ggttatcttc atgttccacg
     6361 ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac gaagctcaat gctgatggtg
     6421 tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga
     6481 ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt ccgcattatt
     6541 tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg agttccatat
     6601 tggctggcat cgcaggttgc gttttggtgt cataaccacc ataaacaacc ttccagtttt
     6661 tgccattctg gctgtaggta atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg
     6721 ttgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga attggcattc
     6781 cggtcaattt gccaatcatc tcttcggcgt catcggcggt ataacgctga cctttattgt
     6841 cgactaactg cacgttaccc ggttgagcat tcagctccag ttccgtgctg cccaatgggt
     6901 tagtgagcag cagacggtag cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca
     6961 ctttttgttg gtcagaaata taagcgaacg cgccgcgagt ctgatactga ttaagattgc
     7021 gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg gcttttgcca ggacctttgg
     7081 gcgtggtaac ggaacaggca gtaagcacaa gagcagccag cggtagcagg cggataagac
     7141 gaaaatcggg caggggcata gtgatgacaa gtccttgaga tacgttgcag ttataaccct
     7201 taatgctagc gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt gccagaatct
     7261 aacggctttc ggcaattact ccaaaagggg gcgctctctt ttattgatct tacgcatcct
     7321 gtatgatgca agcagactaa ccctatcaac gttggtatta tttcccgcag acatgaccct
     7381 tttagcactc ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt
     7441 ttcgccggat aagctcgatc aggcgcttga cagcctgctt gcgcagccga tggtgcaggg
     7501 cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg aagagcagga
     7561 taacctgcaa gaggcgttaa tccgctggct ttgcgattat cacaatctta atgaagaaga
     7621 tctgcgtaaa agcctctact ggcatcagga taacgacgcg gttagccatt taatgcgtgt
     7681 tgccagcggc ctggattcac tggttcttgg ggagccgcag atcctcggtc aggttaaaaa
     7741 agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc gaactggaac gcatgttcca
     7801 gaaatctttc tctgtagcga aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt
     7861 gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc tctctacggt
     7921 cacagtgttg ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga
     7981 acataaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc
     8041 agatgaagtt ggcgcggaag tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc
     8101 cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag gcatggtgga
     8161 gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg ccgttccgcg
     8221 cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagtg tggacgatct
     8281 gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc gcagcggttg aggcggaaac
     8341 tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga
     8401 aaccattcgc gagtatcgca gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc
     8461 gttagcggcc cttgagcagg gcggcgacgc gcaagccatt atgcaggatc tggcatggaa
     8521 actgactaac cgcttgatcc atgcgccaac gaaatcactt caacaggccg cccgtgacgg
     8581 ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc agtacatcat
     8641 tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc caaactggaa
     8701 gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca aactatcgcc
     8761 gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga tgtttcgcgc
     8821 tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat gatgctcgat
     8881 gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag
     8941 caactggaac agcaattaca ggttctgtta ctgccaaaag atcctgatga cgaacgtaac
     9001 gccttcctcg aagtccgagc cggaaccggc ggcgacgaag cggcgctgtt cgcaggcgat
     9061 ctgttccgta tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc
     9121 gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag cggtgatggt
     9181 gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg tgcagcgtgt tcctgctacg
     9241 gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc agaactgcct
     9301 gacgcagaac tgccggacat caacccagca gatttacgca ttgatacttt ccgctcgtca
     9361 ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac tcacttgccg
     9421 accgggattg ttgttgaatg tcaggatgaa cgttcacaac ataaaaacaa agcgaaggcg
     9481 ctttcagtgc tcggtgctcg catccacgct gctgaaatgg caaaacgcca acaggccgaa
     9541 gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac
     9601 aatttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta ccgcctggat
     9661 gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga acatcaggcc
     9721 gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg ttacgtgaag
     9781 caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc
     9841 atgttaccgg cagagggcgt acttttattc tcgcctttgg tgaaacgcag ctgactgacg
     9901 aacaatgtca gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa cccattgctc
     9961 atttaaccgg ggtgcgagaa ttctggtcgt tgctgttatt tgtttcgcca gcgaccttaa
    10021 ttccgcgccc ggatacggag tgtctggtgg agcaggcact ggcgcggttg cctgaacaac
    10081 cttgccgtat tcttgatctc gggacgggta ccggggcgat tgcgcttgcg ctggctagcg
    10141 agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc tgatgctgtc tccctggcac
    10201 aacgtaatgc ccagcatctg gcgatcaaaa atatccacat tctgcaaagc gactggttta
    10261 gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat attgacgagc
    10321 aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg
    10381 cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat
    10441 ccggcggctt tctgcttctg gaacatggct ggcagcaggg cgaagcggtg cgacaagcat
    10501 ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg
    10561 taacgctcgg ccgctattat caatgacaag tttttctaca ctgcttagtg ttcatcttat
    10621 tagtatcgcg ctttctgttg ggctattaac cttacgtttc tggctacgtt atcagaagca
    10681 tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt
    10741 gttaagcggc attgcgttga tggctaaagc gcacatcctg ccattttccg ggcaggcaca
    10801 gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt ttattgcact
    10861 cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc tggcgttggt
    10921 ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg ggtaagtcat
    10981 gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca tgatcctggc
    11041 ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac tggagcgtct
    11101 cgttagtctg gcgaaggaag aaatcagcca gcttctgcct ttagaagagc agctggaaaa
    11161 actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg tttatcgtct
    11221 ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg cggtatcatt
    11281 aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt
    11341 ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc
    11401 ttttaacggt gaatcgttaa gcgaacatat gctggacgta tggttaaagg gaaatatcag
    11461 cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg
    11521 caaattgctg gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt
    11581 acgcaccagc gaagctttat tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg
    11641 cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt
    11701 cgttgaacag tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat
    11761 cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat
    11821 cctatgaaac aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc
    11881 gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag
    11941 cactacgtaa ctgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac
    12001 aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa
    12061 atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca
    12121 agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct
    12181 cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa
    12241 ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc
    12301 aacgaaaaag tgattctttg cgatcgtggt gctaacttcg gctatgacaa cctggttgtc
    12361 gatatgctgg gcttcagcat tatgaagaaa gtgtctggta actcgccggt gattttcgac
    12421 gtgacccacg cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct
    12481 caggtggctg agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa
    12541 gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa
    12601 ctggaaccgt tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa
    12661 ctggatacca gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa
    12721 gacttttttc atgggtgcca gatacaaaaa aggccgcagg ctgttacccc tgcggccggt
    12781 ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac
    12841 gccaccagcc gacaatcgct gcggtaataa ttcccgccag gatcggtgct gccaggtcgt
    12901 gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
    12961 ttttgcggct attcttgaat tgttctggtt caatattagc ccccgttctg ttgtcaggtt
    13021 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
    13081 ccccgcaaca ttgcgcctca ccggataacg ccggcttgat gtggatacta cgtctcaatt
    13141 catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt tttcaacagc
    13201 tgttacagtc gtttcatgag ggctctggat gatgcttcca gctcgggttg ccaatattta
    13261 cttgtggaag agataaagac aaaaatggcc gcaggctgtt acccctgcgg ccggtttcgg
    13321 gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg gttacgccac
    13381 cagccgacaa tcgctgcggt aataatcccc gccaggatcg gtgctgccag gtcgtgccag
    13441 aaagtcatgg caaactgcgc gagcgtcata tagccgcctt gttgtaatga caacattttg
    13501 cggctattct tgaagtgtct ggtttcaaga ttagcccccg ttttgttgtc aggttttacc
    13561 tctcaacgtg cgggggtttt ctctttccag caaccaatgc caccagggat aaagcccccg
    13621 caacattgcg cctcaccgga taattccggc ttggtgtgga tactacgact caattcatct
    13681 tcacttcatc cctgaaatgt ttgcaataaa gagtacattc cggcttttca acagctgttg
    13741 cagtggtttc gtgcaggttg tggtacaggc tcgcaattct gattaacgag ctgtccaggt
    13801 agtatgaaga aatacaataa gcaggaattt atcttatctc ccccgatttc cgtcatcgga
    13861 ggagatataa ctgtcaggca aatatcgtca tcaaataggc ggcaaacagt gccagatgcg
    13921 ctgcgccatt gagcacgtta gtacgtccgg tggagaagga gatatggcac agcactaaag
    13981 aggccaccat caccaccatt tctggcgcac caagtgcaaa ctgtaattcg ttacccgtca
    14041 taaaggcaat tagcgtgacg acaggtacgg taagcgaaat ggttgctaac actgaaccaa
    14101 agaacagatt catcgcgcgc tggacctggt tgttcaacac tgcttttaac gcacctaaac
    14161 cttccggcga cagaatcaac agagccacca ggaagccagt aaaggcgaca ggggcattca
    14221 tgctgtcgag caatgtctcc agcgggctgg cattcatttt ggtgactgca ataacggcaa
    14281 tcagatggat aatcaaccag atagcatgcc acacgctgct atgggcagac ggtttaccgt
    14341 gatgcgggtc atcgtcatca ctgtcatctt cgtgctcgta gacaaacaaa ctttgatgcg
    14401 ttttggtctg gatcagcaaa aatacgccat acattgccgc agaaattaat gctaccagta
    14461 acgcctgacc ggttgaaaaa ttcgccgcag gcagagccat tggaaatacc agtacgatta
    14521 tcgccagggg gaacagcgca attaaatact gcttgatacc aaacagattc atatattggg
    14581 tggcaaactt acgaccgccc aacaataatg aaaagccaac cagcccaccg gtaacaatca
    14641 taatgattga atagagcgta tcacgcatta gcgttggcgc ggcgtcgccg gttgccatta
    14701 aagctgaaat caaactgact tcaagaataa ccactgaaag gctaagaata agcgaaccgt
    14761 aaggttctcc caggcgatgg gctaatacgt ccgcatgacg gacaacacta aaggcgctac
    14821 ttaaaatacc aataagcgca agaagattga tggcaatgac cactggtagt gtctggctac
    14881 ttccccacag gaacagcact accagcgcca gaaccgggaa aataagcgaa gtctccttgt
    14941 ggcgggtttt taccgcctct tgagcatttg acattatggt tatccctttg cagatgaatt
    15001 tatcgaaaat gtaaaaaata ggtaggaaaa ataacagaaa gtgtctggat atcggtaaca
    15061 ttttacgaat ttttaccccg ctgtcgattt tttactcatt ggggcataaa aataagtatt
    15121 acgtttagac aatgtttgtt ttagcgtttc ttaagaagag tctgacatga aaattcttat
    15181 gttttggcaa gagtagatat tgttgaccac acttaatgtt caactttgta aaaggagtca
    15241 acgatgccgt ataaaacgaa aagcgatctg ccggaaagcg taaagcacgt tctaccgtct
    15301 catgcccagg atatctataa agaagcgttc aacagcgcat gggatcaata taaagataaa
    15361 gacgatcggc gtgatgacgc cagtcgcgaa gaaaccgcgc ataaagtggc ctgggctgct
    15421 gtgaagcatg aatatgccaa aggggatgat gataaatggc ataaaaaatc gtaaaaccgg
    15481 tagattagtt aaagctattc gtgcggtgtt gccttgcaag tggtccgtgg attgcatatt
    15541 gtcccgttag tggtttcaaa atgagcagta aaaatgtccg gaagacacca aaaagttgtc
    15601 gcagggaagt atgcagtggc ggaggtgtaa ggtgataacg cgtgatttct tgatgaatgc
    15661 cgattgtaaa acggcatttg gtgccattga agaatcactc ttatggtcag cagaacaacg
    15721 ggcggcttcg ctggcggcga cgctggcttg tcgacctgat gaggaaccgg tgtggatctt
    15781 cggctatgga tcgttgatgt ggaatccggc actggagttt accgaatcgt gcaccggtac
    15841 actggttgga tggcatcgcg cattttgcct gcgcctgacc gccgggcgcg ggactgcgca
    15901 ccagccggga cggatgcttg cactgaaaga gggcggacgc accacaggcg tcgcctatcg
    15961 actgccagaa gagacgctgg agcaggaact aaccttgttg tggaagcgag agatgattac
    16021 cggctgttat ctgccaacct ggtgtcagct tgatcttgat gatggacgca cagtaaacgc
    16081 cattgtgttt attatggatc cgcgacatcc agaatatgaa tctgatactc gcgctcaggt
    16141 catcgcgccg ttgattgcgg cggcgagcgg tccgctggga accaacgcac aatacctgtt
    16201 ttcactggaa caggagctta tcaaactggg aatgcaggac gatgggctga atgatttgct
    16261 ggtatcggta aaaaaactgc tggcggagaa ttttccggat ggtgtgttac gtccgggatt
    16321 cgcctgagta aacttcccgc atagtggggc gtcagacgcc ccctcaaaca ttaaaatgtg
    16381 agcactttat cggctgacag cgtccattgc gccagttcca caagagtacc gatttccacc
    16441 ccatcaatca gaggaagtgt actaatcccg cgcccgtcgg tacaggtttt gcacaatttc
    16501 accggtacat tctgagcggt aaggatctcc agcatttgct gaatgttgta gccttcccct
    16561 ggtttttgcc cgcgcaaccc ggctgtgacc gcatcagaca tgaggaacag acgcagatcc
    16621 agattgctct cctgctctcg taacgcaatg gccagccgca agctgttaaa caaggattcg
    16681 ctcccgtaag gtgcgccatt ggcaacgatc acgatttttt gcattattta ctcctgtatt
    16741 cagggaatta gacactcatc ttctatctta ctgcttctgc agcgtctgac caatcggtca
    16801 catttttaag gattttcctg aaagcgcgag aaaatacgac aaaagttgcc agtaatcgtt
    16861 attctttaag gctatggttt ttcattatta ccggaagtta ccgacgtttt gagccgtttc
    16921 gttcctcgca ttatcccgtt ttatttactc ttgcttgtag caggcggtac agctaacgca
    16981 caatctacct tcgagcaaaa agcggcaaat ccctttgata ataacaatga tggtctgccg
    17041 gatttaggca tggcacctga aaatcatgat ggggaaaaac actttgctga aattgtgaaa
    17101 gatttcggcg aaaccagtat gaatgataac gggctggata ctggcgagca ggcaaaagct
    17161 ttcgcattgg gaaaagtccg cgacgcgctt agtcaacagg ttaatcagca cgtagagtcc
    17221 tggctatcac cgtggggaaa tgccagtgtt gacgtcaaag tggataacga ggggcatttt
    17281 accggcagtc atggaagctg gtttgtgccg ttacaagata atgatcgtta tctcacctgg
    17341 agccagcttg gtcttactca gcaggatgat gggttggtga gcaatgtggg cgttgggcaa
    17401 cgctgggcgc gcggcaactg gctggtgggt tataacactt tttatgacaa cttgctggac
    17461 gaaaatcttc agcgagcggg ctttggtgcc gaagcgtggg gcgaatattt gcgattatcg
    17521 gcaaactttt atcagccatt tgctgcatgg catgaacaga cagccacgca ggaacaacgg
    17581 atggcgcgcg ggtacgacct gacagcccgg atgcgcatgc cgttctatca acacctcaat
    17641 accagtgtca gcgtagaaca gtattttggt gatcgtgttg atttgtttaa ctctggtacg
    17701 ggttatcaca atcccgtcgc gttgagtctg ggattaaatt acacccctgt gccattagtc
    17761 actgtgacgg cccagcataa acagggtgaa agtggcgaga atcaaaataa cctcgggctg
    17821 aatcttaatt accgctttgg tgtaccgctc aaaaaacaac tttctgcggg cgaggttgcc
    17881 gaaagtcagt cgttacgtgg tagtcgctat gacaatccgc agcgaaataa tctaccgact
    17941 cttgagtacc gacagcgaaa aacgttaacg gtgtttctgg cgacaccgcc gtgggatcta
    18001 aaacctggcg aaacagtgcc gctgaaatta caaatccgca gtcgttacgg tattcggcaa
    18061 ctgatttggc agggcgatac gcagatatta agtttgacgc cgggcgcaca agccaacagc
    18121 gcggagggct ggacgctgat catgcctgac tggcagaacg gggaaggggc aagcaatcac
    18181 tggcgattgt ctgtggtggt ggaagataac caggggcagc gtgtctcctc caatgagatc
    18241 acgctaacgc ttgtcgaacc gttcgatgca ttgtcaaacg acgaactgcg ctgggaaccg
    18301 taatcagaaa atgcgctcct gatgcaccca taccgctgct tccacgcgag acttgagctt
    18361 cattttcttc agcatgtgct tgacgtgcac ttttactgtg ctttcggtga tgtccaggcg
    18421 gcgggcaatc atcttgttcg gcaaaccctg ggcaatcagc ttgagaatat cgcgctcgcg
    18481 tggggttaac tggttaacat cgcgctcagt agtggcacgg ttagcacgca agctggcggc
    18541 cagaacaggc gttaatgctt cgcttaatac catttcgcca gcagctgcct gatgcaatgc
    18601 tttcagcaga tcttccggtt ccatatcttt taacagatag ccatccgcgc cgcgtttcag
    18661 tgcggtgacc acatcttctt catggttaga gacgctgaat accacaatgc gccctgagag
    18721 ggacttttcg cgcagtttat ccagcgtttc cagaccgttc atgccgggca tattgagatc
    18781 taacaggatc agatcgggat caagagactc cgccagttca ataccctgtt cgccattact
    18841 cgcttcgcca accacggtga tatctggtgc catactgata agctgtttta cgccagttcg
    18901 cagcatcggg tgatcgtcaa tcagcaggat agtagccggt tcctgattac tcatgggtat
    18961 ctccttggac gtctgtgaaa gttttttcgg gaataaaggt gaccaccact tcggtgccac
    19021 ctgattcacg acggcggacg cggcaatcgc ctcgtaaact ttgcgcacgg tcgcgcatta
    19081 ttatcatgcc gtagtgattg ctgcggatgg cattttcagg cacgccgcag ccgttatcct
    19141 gtacggtcag tttgacctga ttatcgtttt gcgccaccgt caccacgacc tcactcgctt
    19201 gcgaatgttt gagggcgtta cttaatgcct cacgggcaat ttgcaacaag tggattgcct
    19261 gatgcgaagg caccagacga ggcggcaatt gataatccag cttcaccggg aagccaaatt
    19321 tggcgctgta ctcttcgcaa ctcgcctcca gcgccggacg taatccaggc tcggtgagct
    19381 gcaagcggaa tgtggtgagc aattcacgca actgcgccca ggatgcattc agttcgttac
    19441 ggatctgact taacagttcg cggctgcttt ctggcagcgc atcgccctgc atctgtaaac
    19501 aactcacctg catcttcata caagagagag attgggcaat agaatcatgc agttcgcgcg
    19561 caatggtggc acgctcttcc atcacgatca actgttgctg acgttcctga tggcgatcca
    19621 gcgccagcgt ggcggtgagt tgttcaacca gggtatccac cagttgttgt tgatcatggc
    19681 taagatgacg cccctgcggc agggtcgcca gcaaaatacc gtactgcgta tgagagtcag
    19741 ccagccgcca cttcagggtc gtgccgcgat caccaacggg taatacgccg cgcgggcaga
    19801 gctggcagcc tttatcatca caagtcatat ctggctggca ggtaaactcc tgatgattct
    19861 cttcatcatc agtgtcatac acccgcaatt cgatatcacg tagcagggtt aaattctgta
    19921 agccgttgag tacaggtgac aggcgttcac acagcggggc gcgggaatgc aaacggcggt
    19981 tagcctgcca taaaaaagag aggatctgat ttttatgctc cagcccggcg gttttctcct
    20041 gaacccgctg ctcaagtacg gcataacttt cggccagttc tgcagacata ttgttcaacg
    20101 cagttccaag catcgccatt tcgttgcgcc cgctgatgtt tgcgcgttgg gtaaaatcgc
    20161 gatgactgac ggcactcgcc attgccagca gttgccgcca cggttgtagc agtcgcgccc
    20221 gcaaccagat aatagtgaac accagtaaaa gtgccataaa taccgccatt acccgatgga
    20281 ccagtaccac tgtctcgatg cgcatttccg tggtgcggtc aaaaccagat accagttgat
    20341 caagcccggc aacaaactgg ctgacatccg ctgacaccgt ttctcggttt tgtgcacgca
    20401 tcaacgcagg gatcagttca ttacgccagt aatcttgtaa accctgtaat tgcgccagtt
    20461 gtccgtctcg ttctgctgct cgagtcaact cggcgctaaa tgccgtttgt tccatctctt
    20521 taattaaggg cttgtctttc tcgcttaatg gcactgctgc caacagacgg taactttgca
    20581 tgcgcagcga tcccgctttg ttgatcgcat gggcgctgcc ctgaacgcct tgcaccagcc
    20641 agccagaaac cgccatccct gccagtccaa tagcagtaga aagcaacaca ataagcgcaa
    20701 cctgattaac cagggtgagc ggagagagac aacgtttaag catgtaaacc tcttccttca
    20761 ggctttaaat gagcaataac cttaatgaat gtgacgatac attctggaat ggcagtattc
    20821 tcggctattg gctgaagtat acccataccc ggaaagagtt actccttatt tgccgtgtgg
    20881 ttagtcgctt tacatcggta agggtaggga ttctacagca ccgtgaaaaa tctcataatt
    20941 tttatgaaat cactgtactc actatgggta atgataaata tcaatgatag ataaagttat
    21001 cttatcgttt gatttacatc aaattgcctt tagctccaga cactaaggtg gcagacatcg
    21061 aaacgagtat cagaggtgtc tatgagtcac tcatctgccc ccgaaagggc tactggagct
    21121 gtcataacag attggcgacc ggaagatcct gcgttctggc aacaacgcgg tcaacgtatt
    21181 gccagccgca acctgtggat ttccgttccc tgtctgctgc tggcgttttg cgtatggatg
    21241 ttgttcagcg ctgttgcggt gaacttaccg aaagtcggtt ttaattttac gaccgatcag
    21301 ctatttatgt tgactgcgct gccttcggtt tctggcgcgt tattacgtgt tccatactcc
    21361 tttatggttc ctatcttcgg tggtcgtcgc tggacggcgt tcagcaccgg tattctgatt
    21421 attccttgcg tctggctggg ttttgccgtg caggatactt ccacgcctta tagcgtcttc
    21481 atcatcatct ctctgctatg cggctttgct ggcgcgaact tcgcatccag tatggcaaac
    21541 atcagcttct tctttccgaa acagaagcag ggtggcgcgc tggggctgaa tggtggtctg
    21601 ggaaacatgg gcgtcagcgt catgcagttg gttgctccgc tggtggtatc actgtcgatt
    21661 ttcgcagtat ttggtagcca gggcgtcaaa cagccggatg ggactgagct gtatctggcg
    21721 aatgcgtcct ggatatgggt gccgttcctt gccatcttca ctattgcggc gtggtttggc
    21781 atgaacgatc ttgctacctc gaaagcctcc atcaaggagc agttgccggt actcaaacgg
    21841 ggtcatctgt ggattatgag cctgctgtat ctggcaacct tcggctcctt catcggcttc
    21901 tccgcgggct ttgcaatgct gtcaaaaacg cagttcccgg atgttcagat tctgcaatac
    21961 gctttcttcg ggccgtttat tggtgcgctg gcgcgttctg caggtggtgc attatctgac
    22021 cgtctgggcg gaactcgtgt cacgctggtg aactttattc tgatggcgat tttcagcggc
    22081 ctgctgttcc tgaccttacc gactgacggg cagggcggaa gcttcatggc gttcttcgcg
    22141 gtcttcctgg cgctgttcct gacagctggg ctgggtagtg gttccacttt ccagatgatt
    22201 tcagtgatct tccgtaaact gacaatggat cgcgtgaaag cagaaggggg ttctgacgaa
    22261 cgtgcgatgc gtgaagcggc aaccgacacg gcagcggcgc tgggtttcat ctctgcgatt
    22321 ggcgctattg gtggcttctt tatcccgaaa gcgtttggta gctcgctggc attaacgggt
    22381 tcgccagtcg gcgcaatgaa ggtatttttg attttctata tcgcctgcgt agtg
//
LOCUS       DAZFAH010000061        22120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFAH010000061 DAZFAH010000000
VERSION     DAZFAH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.061"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..805
                     /locus_tag="ACHJWB_002207"
     CDS             <1..805
                     /locus_tag="ACHJWB_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4313845.1"
                     /translation="QLQQQVTDLTSSLNSQEKKELTHKLESIFNNTQVQIAVLIVPTT
                     KDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEEKVTDALAGDI
                     IRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESESASSSDHYYFAIFWV
                     FAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIFSIAVFSFFFTFTIFM
                     VFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(865..2163)
                     /gene="eno"
                     /locus_tag="ACHJWB_002208"
     CDS             complement(865..2163)
                     /gene="eno"
                     /locus_tag="ACHJWB_002208"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4313846.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2251..3888)
                     /gene="pyrG"
                     /locus_tag="ACHJWB_002209"
     CDS             complement(2251..3888)
                     /gene="pyrG"
                     /locus_tag="ACHJWB_002209"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4313847.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4116..4907)
                     /gene="mazG"
                     /locus_tag="ACHJWB_002210"
     CDS             complement(4116..4907)
                     /gene="mazG"
                     /locus_tag="ACHJWB_002210"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4313848.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4978..5313)
                     /gene="mazF"
                     /locus_tag="ACHJWB_002211"
     CDS             complement(4978..5313)
                     /gene="mazF"
                     /locus_tag="ACHJWB_002211"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4313849.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5313..5561)
                     /gene="mazE"
                     /locus_tag="ACHJWB_002212"
     CDS             complement(5313..5561)
                     /gene="mazE"
                     /locus_tag="ACHJWB_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4313850.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5639..7873)
                     /gene="relA"
                     /locus_tag="ACHJWB_002213"
     CDS             complement(5639..7873)
                     /gene="relA"
                     /locus_tag="ACHJWB_002213"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4313851.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7921..9222)
                     /gene="rlmD"
                     /locus_tag="ACHJWB_002214"
     CDS             complement(7921..9222)
                     /gene="rlmD"
                     /locus_tag="ACHJWB_002214"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4313852.1"
                     /translation="MAQFYSAKRRSTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9279..12035
                     /gene="barA"
                     /locus_tag="ACHJWB_002215"
     CDS             9279..12035
                     /gene="barA"
                     /locus_tag="ACHJWB_002215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4313853.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12267..13607)
                     /gene="gudD"
                     /locus_tag="ACHJWB_002216"
     CDS             complement(12267..13607)
                     /gene="gudD"
                     /locus_tag="ACHJWB_002216"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4313854.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTHNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13628..14968)
                     /locus_tag="ACHJWB_002217"
     CDS             complement(13628..14968)
                     /locus_tag="ACHJWB_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO4313855.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14970..16322)
                     /gene="gudP"
                     /locus_tag="ACHJWB_002218"
     CDS             complement(14970..16322)
                     /gene="gudP"
                     /locus_tag="ACHJWB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4313856.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16756..17205)
                     /locus_tag="ACHJWB_002219"
     CDS             complement(16756..17205)
                     /locus_tag="ACHJWB_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4313857.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17223..18005)
                     /gene="truC"
                     /locus_tag="ACHJWB_002220"
     CDS             complement(17223..18005)
                     /gene="truC"
                     /locus_tag="ACHJWB_002220"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4313858.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18005..18334)
                     /locus_tag="ACHJWB_002221"
     CDS             complement(18005..18334)
                     /locus_tag="ACHJWB_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4313859.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18956..19501)
                     /gene="syd"
                     /locus_tag="ACHJWB_002222"
     CDS             complement(18956..19501)
                     /gene="syd"
                     /locus_tag="ACHJWB_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4313860.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19569..20417
                     /gene="queF"
                     /locus_tag="ACHJWB_002223"
     CDS             19569..20417
                     /gene="queF"
                     /locus_tag="ACHJWB_002223"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4313861.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20529..21893
                     /gene="ppnN"
                     /locus_tag="ACHJWB_002224"
     CDS             20529..21893
                     /gene="ppnN"
                     /locus_tag="ACHJWB_002224"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4313862.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSIP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5445 a         5892 c         5243 g         5540 t
ORIGIN      
        1 acaattacag caacaagtta cggatcttac gagtagctta aactcacaag aaaagaaaga
       61 actgacgcac aagttagaat ctattttcaa taatacccaa gtgcaaattg ctgtattaat
      121 tgttcccaca accaaagacg agacaattga acaatatgct acaagagttt ttgacaattg
      181 gcgtttagga gatgccaaac gtaatgatgg gatactgatc attgttgcct ggtcagatcg
      241 tactgtccgc atccaggtag gttatgggct ggaagaaaag gtaaccgatg ctctggctgg
      301 agatatcatc cgtagcaaca tgatacccgc atttaaacaa caaaaatttg ctcagggatt
      361 agagctagct ataaacgctt tgaataacca actcacttca caacaccaat atccgactaa
      421 tccttcagaa agtgaatcag cgtcttccag tgatcattat tactttgcta ttttttgggt
      481 atttgcagtg atgttcttcc ctttctggtt ttttcatcaa ggcagtaatt tttgtcgcgc
      541 atgtaaaagt ggagtctgta tttcagcaat ctatctttta gatttattcc tgttctccga
      601 taaaattttt tccattgctg tattttcctt cttttttact ttcaccatat ttatggtctt
      661 tacctgttta tgtgtacttc agaaaagagc atcaggtaaa agttatcatt cagacaacag
      721 cggttccggt ggaggctcaa gtggcggcgg atttagcggc ggtggcggtt cttctggagg
      781 tggcggcgca tccggccgct ggtaactcaa ccataaaaaa tgccagcccg gaggctggca
      841 tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac gaccgttgta
      901 cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt tagcaacacg
      961 gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa cagccaggtc
     1021 agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag tgtagccagc
     1081 atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct ggttgaattt
     1141 gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct tggtgttggt
     1201 tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg tctggtatgc
     1261 gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gaaacgatcg ggtactgttt
     1321 ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aacgctttgt tgccttcgcc
     1381 agccagaacg tatttaccat ctttgtagaa ttcagaagct gcgcagtcca tcgccagagt
     1441 gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa cagccagagc
     1501 ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaacag cagtgttcat
     1561 gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca tgcggatggc
     1621 ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat caacgttgtt
     1681 gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatag agtatttgcc
     1741 cggagtaccg ttcagttcag cgatgtgctc gtacagcggc atacctttag cagctgcagc
     1801 agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt tggatttgtt
     1861 ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt tagcatcttt
     1921 gccaatcagc gcctgagcga tcgggccgtt taccgcagca acagctttgg ttacgccttt
     1981 acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg aaccagtaga
     2041 agcacctgac ggagcagctg ccataccgac gaacccaccc tccagatgta cttcggcttc
     2101 aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta cgattttgga
     2161 cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa cgcgtaccca
     2221 gggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct ggaactcgct
     2281 ggcggctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag aagtaaactc
     2341 cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga tctcgaccaa
     2401 ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct gtttcaacag
     2461 catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg cattatacag
     2521 ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac gcatggtacc
     2581 gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc gccactcggt
     2641 aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt tggcgttctc
     2701 catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca ggcaaatgcc
     2761 cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc cttctacgcc
     2821 acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga tttcaacgcc
     2881 gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac gattcttcag
     2941 cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt caatgtactt
     3001 gccgaccata ccgatggtga cttcacttac cgggttcgct tcttcgaaga taacctgttc
     3061 ccattcggac agattcgctt ccgggcagtt taagctgaat cgtttacaaa tataatcgtc
     3121 cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt ctttcagaga
     3181 aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt cgttcgccgg
     3241 aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg agagcagctc
     3301 ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca tgtacggcac
     3361 cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca tctggcgaat
     3421 cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt ctaccagtac
     3481 tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag tgatgtgcgg
     3541 aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca gaacgtcaga
     3601 gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa tgaaacgctc
     3661 gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca cttccccgtg
     3721 ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt tcatgatggt
     3781 cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa tgcctttacc
     3841 cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc tgaacctgag
     3901 aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac ccgaacatga
     3961 cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga aatcaggagt
     4021 gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt gtcatttaaa
     4081 aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc ctgccgtttt
     4141 acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc cagtccacgc
     4201 gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt cgctttttgc
     4261 aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc aaacagcagg
     4321 tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc ctcgtacatc
     4381 acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt ccagtcgaag
     4441 ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg taaactacga
     4501 ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt tttgatttgc
     4561 tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa aacatgcgga
     4621 tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc aaagcgccct
     4681 tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc cagttcaccg
     4741 cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt ttcttcaagg
     4801 gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc gttttccgga
     4861 tcgcgcaggc gctgcataat agtgagcaaa cgatcgattt gattcattga attgtcctga
     4921 aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt agtaacacta
     4981 cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg caactgttcc
     5041 tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag ctaacgctac
     5101 gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg attgcgttgt
     5161 acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac tcaggacaac
     5221 agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa cccaaatcag
     5281 atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc tttcggctct
     5341 ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag ttcagcaagc
     5401 gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac caggtcaatc
     5461 ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg gatccgcacc
     5521 gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc ctcaaaccgc
     5581 tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa atttctgcct
     5641 aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt ttacccagca
     5701 cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt gccagttgct
     5761 gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg gcgagaatgg
     5821 tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc accagcgaat
     5881 atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc gcatgggagc
     5941 gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc ccctgggtaa
     6001 tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg tggtgcatta
     6061 ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt tgcggcgtgt
     6121 agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca ctcggcttat
     6181 taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg ccaccaatcg
     6241 ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt ttttctgctt
     6301 ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc ccagccagaa
     6361 tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg
     6421 ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc tgtttctggg
     6481 tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca atgcgcccgc
     6541 caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa gcgaagtcca
     6601 gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag acatacaccc
     6661 ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca gccatctctt
     6721 cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc gaacgtgcgc
     6781 cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac tctgcatctt
     6841 catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc cccagaacca
     6901 cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg tcaaactcat
     6961 ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa tcctgtaaac
     7021 gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg aggttctttt
     7081 tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact tcagctttaa
     7141 cgccttcagc tttcatctca gcgcgcagat gaccgacaaa ctcttcgata tagtgttcgc
     7201 ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc gggtgcagat
     7261 aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga cggttcgcca
     7321 acggcgcgta gatattggtg cactcttttg ccgccagtac gcgttcatct tccggcgcat
     7381 cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgaccacg cagcgaaaat
     7441 catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag gaaacagaat
     7501 cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg tgaataaggt
     7561 taacaaccga cttaccgacg ctctcacgca gcacatcttc gctaactacg ttggcatcag
     7621 ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat gtcgagagga
     7681 tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc ccctgcgtct
     7741 gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg ctggtaatac
     7801 ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata tgtgcacttc
     7861 ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg caaatcgtaa
     7921 ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc
     7981 cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc tatcccgagc
     8041 cagcgttgca ggattacagg atacataaac tatacgaata ggttccagtt ttataatttg
     8101 ctgcataaca cctgcggcac ctgctcgcgc ggggtccagc aacactttat cgaagccgtt
     8161 tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcatgataaa acgtcacatt
     8221 ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg gaacaccttc
     8281 tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc ccataccgca
     8341 gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg cacgcgctac
     8401 cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa aagttaagcg
     8461 caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta tctcactatc
     8521 gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt tttcgcgatc
     8581 agccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg ttgcctgtac
     8641 cagttcaaca tgaccaagat ggcgtatagc ttgtaagctg cccaggcatg ccctgacttt
     8701 gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt cgacaatgtc
     8761 actggagccc gctttgcgaa agcccatctg aagttgctgt gttttcggta agtagttcaa
     8821 acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt cagagacttc
     8881 gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat ccacgctggc
     8941 gtgttgttgc tgacagccac cgcatacgcc aaaatgaggg cagcgtggcg tttcgcgttc
     9001 cgggctatcg cttaaccggc gcacgacttt agcgcgggcg tactgttttt tatcttcagt
     9061 aacagtaact tccgcgtttt cctgtggcag taatccagga ataaataacg ctttgccgtt
     9121 atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg ttatgatctg
     9181 acgcgtcgtc gagcgtcgtt ttgcagagta gaattgcgcc attggcgaga ctttctcaat
     9241 ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac agcctgcgcg
     9301 cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg ctgagtatct
     9361 ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc ggtgccagca
     9421 ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat cgcgaatcta
     9481 tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc gcgatttcgg
     9541 tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat ccctcatcaa
     9601 tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt gacggcgata
     9661 ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa tcgcccagta
     9721 gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg gatcttaaat
     9781 cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg ctgttttgta
     9841 tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt ccgattcgca
     9901 acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg gtggaaggat
     9961 ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg
    10021 ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat ctgcgtgaaa
    10081 cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag cgcgcccagg
    10141 aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg cgtacaccac
    10201 tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca ccaacgcagc
    10261 gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt attaatgatg
    10321 ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt ccattcccac
    10381 tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac gataaagggt
    10441 tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc gacccactgc
    10501 gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc gagaatggca
    10561 acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag attgaagtgc
    10621 agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta ttccaggcct
    10681 tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg gggctggtga
    10741 ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc cagccgaatc
    10801 gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt attatcgaag
    10861 ggccatccac ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca aactccgcag
    10921 cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt tatagcccaa
    10981 cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc gcggtgacct
    11041 tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg atgaccgatt
    11101 tcctgatgct ggcacttcct tgccatgcac aagtcaatgc tgaaaaactc aagcaagatg
    11161 gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct gccctgacgg
    11221 aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc aagctggcaa
    11281 tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc gcattgctgg
    11341 aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt gaacgggcga
    11401 aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg gatggcattc
    11461 gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt atcgcggtaa
    11521 cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg agcgattatc
    11581 tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac aagcctggca
    11641 gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg aacccgaatg
    11701 cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg
    11761 atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt gaggaacagc
    11821 tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat ggcagttgcg
    11881 gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa cagctacgta
    11941 gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac gagatggata
    12001 atgtagcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga gtagcaatcg
    12061 gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg ccggatgcgg
    12121 cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa aaatgatgta
    12181 gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa gccggctcca
    12241 gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt gttatcgaac
    12301 gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc aagcccgtgt
    12361 ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac acccagcccc
    12421 ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt ggtcaagcgc
    12481 tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc cggtgcagcg
    12541 gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga gtgtgaaccc
    12601 caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg catcgtccag
    12661 aaatgcggat ccgccagcgg gatatcaacg gattgcaggg agagcgtatg gcccatctgc
    12721 cgccagtcgg tggcgatcat attggttgcg gtcggcaggc cagttgcgcg acggaactct
    12781 gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc tgcataagcc
    12841 agcgaccctt tcaggtattt accaatttta atcgcttcgt tcagcgacca ggcaccgtta
    12901 ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat agactcggcc
    12961 tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc atatttttca
    13021 tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc atgacgcagg
    13081 cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagcggtgt ggctttgcga
    13141 tcaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg accatcgccc
    13201 agcagggatg ccacgtttac ccccagatgc tgccccagca gatccagcat tgccgcttct
    13261 atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc gcgcccacca
    13321 gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta ttcacccagc
    13381 gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt ctcgccgccg
    13441 ggaatttccc ctacgccagt gtgaccagaa ttatctttga taatcacaat attatgcgta
    13501 aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc cgccaccggg
    13561 atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt tatgtccttt
    13621 ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag ccggggatca
    13681 ggtactgcat cggacctgcg tcgttacgcg caccgacagg cagacgttta taggcctcat
    13741 gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca tcaggaacgg
    13801 caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg ccctcctgcc
    13861 aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc acatgggtaa
    13921 acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc agcccccagt
    13981 cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc ggatcggcaa
    14041 gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag ttggtggcga
    14101 tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata acttcacgac
    14161 cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca tcattcagcc
    14221 ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca acggtaatcc
    14281 gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc tcgccaggta
    14341 acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag gcttccgcca
    14401 gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac cactcatggt
    14461 tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg atataaaaca
    14521 gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt tcgcagaccg
    14581 gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc gccacggcat
    14641 taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg gctgcctgat
    14701 tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg cccagaacca
    14761 tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct tcaccaatgc
    14821 cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat gcgttatgtg
    14881 ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact ttcatatcag
    14941 taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt cagctcgata
    15001 cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc atgaacacca
    15061 acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc aattgcgatt
    15121 ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga aagaccgctg
    15181 atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat gcccttaccg
    15241 aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta gttgcagaac
    15301 accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc aatgttcagc
    15361 gatcccgtgc ggcgcatcag ccagtcggaa ataatcccac ccagcacacc gccgataaaa
    15421 ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat cccgcgtgcc
    15481 tgcaccagat aaaccgggaa ccaggtaata aagaagtaag tcagggcgtt gatacagtac
    15541 tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat ctgcccccac
    15601 ttcacgctga acggcacttt aactttagtg ttttgctgat ccatattgat cagcgcgcca
    15661 cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt tggctcgtgg
    15721 atgactttca accagataaa gctgatgaca atccccagac cgcccataaa gaagaagacg
    15781 tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat caccgttgcg
    15841 aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc cggaaaccag
    15901 gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc gaccaggaag
    15961 cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac gaagccttgc
    16021 agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt tgaaccaaaa
    16081 cggtccagca accagccacc agggatctgc ccgataacat aagcccatga gaaggcagag
    16141 aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc cgaaccggca
    16201 atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa catcaccact
    16261 atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact taaagaactc
    16321 attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg acatagccag
    16381 gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg ccgaatacgg
    16441 cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct caccgtgcaa
    16501 caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg gacggtggaa
    16561 tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga agaaataaag
    16621 aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc gcgctatgaa
    16681 gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa acgacattta
    16741 ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg gatttgattc
    16801 cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa cccgctgagc
    16861 gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcagaaat ttacataact
    16921 gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca gactatcttt
    16981 gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg tcgtggacgt
    17041 aaccaccaga acatacttat cctgataagg cagccagtcg cttaattcag gatcttcaaa
    17101 taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg ccactaacag
    17161 cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt tttctccctg
    17221 gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac cctttcattt
    17281 tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt gtcgtccagg
    17341 cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa ctgactggca
    17401 tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg gcgtaaatcg
    17461 ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg gcgaagctga
    17521 tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt cgggtaacgt
    17581 ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac tgctggctgc
    17641 gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc caccagtgga
    17701 taatccagca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta acgtttctgg
    17761 atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga taatcccatc
    17821 aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg ctgtcctatc
    17881 tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca gctacggtga
    17941 accagccagc cggagggttt atttaccgca accagccatt catcctgata gagtatttcc
    18001 agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca gccagaataa
    18061 gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca acagcaaaag
    18121 cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc agcacccact
    18181 gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta ttaaattgat
    18241 gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct tcaagcgcct
    18301 gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa gaaaaactgc
    18361 cgcgaagaat agcaggaata aaaaaaggga gcactgtatt cacagcgctc ccggttcgtt
    18421 tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt ttcctctggc
    18481 cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc cgatgtgata
    18541 cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac cggttctcat
    18601 tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct tcatccagaa
    18661 gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc ctgatgttca
    18721 cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga agtcttacaa
    18781 ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat taacaatcaa
    18841 cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc ttatttcctg
    18901 gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat ttggattaca
    18961 gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc gtccgtttac
    19021 gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag atgacctcta
    19081 actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt ttctgagtca
    19141 ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc caggtttgca
    19201 gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac tgagtcgtat
    19261 aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc gcgtttacat
    19321 tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta gttgaaataa
    19381 tgcacggtga aggaacaccg tacagttcct cacttaacgg ccagctttta tgctcttcat
    19441 gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc aaatcgtcca
    19501 ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct ggtttatgac
    19561 ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact cttggaaaat
    19621 caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca cgcagcctga
    19681 atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg gatatctgga
    19741 cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggttgcc gtcggtcacg
    19801 ttgaacttga ttacaccagc gcaaatctga ttgagtcgaa gagttttaag ctctatctca
    19861 acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg ctggagcgcg
    19921 acttaagcac ttgcgcccag ggtaaggtta gcgtggcgtt atatcgtctt gatgaactgg
    19981 aaggccagcc gataggtcat tttaatggca cttgcattga tgaccaggat atcactatcg
    20041 ataactatga attcactact gactatctgg agaatgccac ctgtggtgaa aaagtagtgg
    20101 aagagacgct tgtcagccac ctgctgaaat caaactgcct gatcacccat caaccagatt
    20161 ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa ctgctgcgtt
    20221 acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa cgcatcttta
    20281 atgacctgtt acgcttctgc cagccagaaa aattgagcgt ttacgcacgt tatacccgtc
    20341 gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca tcgaccacaa
    20401 gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc agaaggttgt
    20461 gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg tcccataagg
    20521 agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg tcgcagctgg
    20581 aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt cgcaactgtt
    20641 cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg ctgtctcgtt
    20701 ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg gaactgatta
    20761 atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag gccaacttgt
    20821 tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataacacc gttcgttttc
    20881 ccaacctgaa tctcgacaac tccgtacaca tcactaacct ggtcttttcc atcttgcgta
    20941 acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg ggcggtcact
    21001 caattaacga aaatgagtat ttgtatgccc gtcgcgtcgg aaaccagctg ggcctgcgtg
    21061 agctgaatat ctgcaccggc tgtggtccgg gagcgatgga agcgccgatg aaaggtgctg
    21121 cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg acagagccgt
    21181 cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc atgccagata
    21241 tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg tatcattatc ttccctggcg
    21301 gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac ccggccaaca
    21361 aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac tacttccgcg
    21421 tactggacga gtttgtcgtg catacgctgg gtgaaaacgc gcgccgccat taccgcatca
    21481 tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgataccg ctggtgaaag
    21541 aaaatcgccg cgacacaggc gatgcctaca gctttaactg gtcaatgcgc attgcgccag
    21601 atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag ctttacccgg
    21661 atcaacccgt tgaagtgcta gctgccgacc tgcgccgtgc gttctccggt attgtggcgg
    21721 gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac aaaatcaacg
    21781 gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt gcccagcatc
    21841 gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg taatctcccc
    21901 tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacttactc atcaactcat
    21961 ttcatttgtt atatgaatgt ttcttaccac cctcacgcga caaatatcat cacagttaat
    22021 atgtcataca atttatgttg caacgcaaac gtttccctat tttcataaac cgtattttat
    22081 cgctatgaaa aagaaattat cgccatgatt aactaaaagt
//
LOCUS       DAZFAH010000062        22075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFAH010000062 DAZFAH010000000
VERSION     DAZFAH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.062"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1013)
                     /gene="malZ"
                     /locus_tag="ACHJWB_002225"
     CDS             complement(<1..1013)
                     /gene="malZ"
                     /locus_tag="ACHJWB_002225"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4313863.1"
                     /translation="MMLNAWHLPVPSFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDV"
     gene            complement(1169..2542)
                     /gene="proY"
                     /locus_tag="ACHJWB_002226"
     CDS             complement(1169..2542)
                     /gene="proY"
                     /locus_tag="ACHJWB_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO4313864.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2618..3937)
                     /gene="brnQ"
                     /locus_tag="ACHJWB_002227"
     CDS             complement(2618..3937)
                     /gene="brnQ"
                     /locus_tag="ACHJWB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4313865.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4344..5639)
                     /gene="phoR"
                     /locus_tag="ACHJWB_002228"
     CDS             complement(4344..5639)
                     /gene="phoR"
                     /locus_tag="ACHJWB_002228"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4313866.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5697..6386)
                     /gene="phoB"
                     /locus_tag="ACHJWB_002229"
     CDS             complement(5697..6386)
                     /gene="phoB"
                     /locus_tag="ACHJWB_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4313867.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6576..7778
                     /gene="sbcD"
                     /locus_tag="ACHJWB_002230"
     CDS             6576..7778
                     /gene="sbcD"
                     /locus_tag="ACHJWB_002230"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4313868.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7775..10918
                     /gene="sbcC"
                     /locus_tag="ACHJWB_002231"
     CDS             7775..10918
                     /gene="sbcC"
                     /locus_tag="ACHJWB_002231"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4313869.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11044..12228
                     /gene="araJ"
                     /locus_tag="ACHJWB_002232"
     CDS             11044..12228
                     /gene="araJ"
                     /locus_tag="ACHJWB_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4313870.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12371..13279)
                     /gene="mak"
                     /locus_tag="ACHJWB_002233"
     CDS             complement(12371..13279)
                     /gene="mak"
                     /locus_tag="ACHJWB_002233"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4313871.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTRVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13404..14315
                     /gene="rdgC"
                     /locus_tag="ACHJWB_002234"
     CDS             13404..14315
                     /gene="rdgC"
                     /locus_tag="ACHJWB_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4313872.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14393..14476)
                     /locus_tag="ACHJWB_002235"
     CDS             complement(14393..14476)
                     /locus_tag="ACHJWB_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313873.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRRE"
     gene            complement(14473..14814)
                     /locus_tag="ACHJWB_002236"
                     /pseudo
     CDS             complement(14473..14814)
                     /locus_tag="ACHJWB_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14962..15246)
                     /gene="ppnP"
                     /locus_tag="ACHJWB_002237"
     CDS             complement(14962..15246)
                     /gene="ppnP"
                     /locus_tag="ACHJWB_002237"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4313874.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15318..15995)
                     /gene="aroM"
                     /locus_tag="ACHJWB_002238"
     CDS             complement(15318..15995)
                     /gene="aroM"
                     /locus_tag="ACHJWB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4313875.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16253..16444)
                     /gene="yaiA"
                     /locus_tag="ACHJWB_002239"
     CDS             complement(16253..16444)
                     /gene="yaiA"
                     /locus_tag="ACHJWB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4313876.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTVQEAMDAKKRYEDPDKE"
     gene            complement(16494..17018)
                     /gene="aroL"
                     /locus_tag="ACHJWB_002240"
     CDS             complement(16494..17018)
                     /gene="aroL"
                     /locus_tag="ACHJWB_002240"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4313877.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17201..17659)
                     /gene="yaiI"
                     /locus_tag="ACHJWB_002241"
     CDS             complement(17201..17659)
                     /gene="yaiI"
                     /locus_tag="ACHJWB_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4313878.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQNGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17779..18588
                     /gene="proC"
                     /locus_tag="ACHJWB_002242"
     CDS             17779..18588
                     /gene="proC"
                     /locus_tag="ACHJWB_002242"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4313879.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18605..19720)
                     /gene="adrA"
                     /locus_tag="ACHJWB_002243"
                     /gene_synonym="dgcC"
     CDS             complement(18605..19720)
                     /gene="adrA"
                     /locus_tag="ACHJWB_002243"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4313880.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19822..20142)
                     /gene="psiF"
                     /locus_tag="ACHJWB_002244"
     CDS             complement(19822..20142)
                     /gene="psiF"
                     /locus_tag="ACHJWB_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4313881.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20261..21676)
                     /gene="phoA"
                     /locus_tag="ACHJWB_002245"
     CDS             complement(20261..21676)
                     /gene="phoA"
                     /locus_tag="ACHJWB_002245"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO4313882.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21777..22037)
                     /gene="iraP"
                     /locus_tag="ACHJWB_002246"
     CDS             complement(21777..22037)
                     /gene="iraP"
                     /locus_tag="ACHJWB_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4313883.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         5542 a         5934 c         5512 g         5087 t
ORIGIN      
        1 acatccagcc gccagccgtc catactccac ggcgctttca gccaatggcg gacaatactg
       61 tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag cttcggcaag
      121 ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc
      181 cagggcgatt cagggttgtg acaagctccc cccgtgccac gattgtgcct gtcaaaccag
      241 gcatgggaat cgccactgtg gttaaatacg ccgtccagca ccagccgcat tcccagctgc
      301 tgcgtattgt gtcgcaaacg cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg
      361 cgataatcct cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac
      421 agcgccgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg
      481 ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag
      541 atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga
      601 agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt
      661 gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg
      721 aagccctgcg gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc
      781 cgccggggtt gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gcctggctgc
      841 ggctgactgc gctgcttatg cattggtact gacatttctt cgttatcgtg ttctgtacgc
      901 agcataatgc gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct
      961 ttgctttgtt taacaaatga gggcaccggc aggtgccatg catttaacat cataattccc
     1021 ctgtgatgaa aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag
     1081 gatatttggg ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc
     1141 tcctgaaccg gcttacagaa cgcaagtatt attggttttc agccagctga cgatcgtggc
     1201 gacgcttaaa catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg
     1261 agatacgcgt atccgggtga taaccaatca acccgataat aaagagcagg aaaatcagcc
     1321 cgccgatggt cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg
     1381 gcggcaaacg gcgacggaag gcaatttgcg acagcaggat cataatccac acccacaccg
     1441 tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca
     1501 gatacaccgc aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc
     1561 gacgcgacgt tttgctgaaa attttcggcg cgctgccctg ctctgccata ccgtggagca
     1621 tacggcctac gccaaataca tcactgttaa ttgccgacag cgaagcagtc agcacaacaa
     1681 agttaagaat gctggcggca aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc
     1741 taccggcagt gccaacctga ttccacgggt agatagacat aatgacgaac agcgtaccga
     1801 cgtagaacac cagaatacgc atcggcacgg agttaatcgc acgcggtatc gatttctcag
     1861 gatctttcgc ttcaccggcg gtaatcccga taatttcgat cccaccgtaa gcaaacatca
     1921 ccatttgcaa cgacattacc atgccaagcc agccgttact gaagaagccg ccgttgctcc
     1981 acaggttatg aataccggtc ggttgcccgc cgttgccaat cccccagatg atgatgccga
     2041 aaccggcgac aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca
     2101 gctcaccgaa taccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca
     2161 gtacccaaat ccagtgcggc accgtcggga accagacacc catatagata ccaaaagcgg
     2221 tcacatcggc gatggcgaca ataagaattt caaagcagta ggtccagccg gtaatgtaac
     2281 ctgccagcgg gccgaggttt tcctgcgcat aacgcgagaa agagctggcg gccgggttat
     2341 gtaccgacat ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat
     2401 aggccaacaa cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc
     2461 cggtgccaat tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct
     2521 ttagcttgtt cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg
     2581 catcgcagcc ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct
     2641 gacgacctgc cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta
     2701 accacgccag accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc
     2761 tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg
     2821 gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg
     2881 cgatacacgg cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta
     2941 agccgaggtt agaaaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag
     3001 agagcggtac gtactgggca aagaattctg cacaagcaca ggtcaggcca actgccgtta
     3061 ccaggcaggc gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt
     3121 gctgaacgta agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc
     3181 tgtctgaacc cagacggaac agcgccaggt acagcagagt cagaccaaca cccgccatca
     3241 ggccagccca gacggtataa cgggtcagta ggcgcgcttc ggtaacgcca cgagaacgcg
     3301 ccgcgttaac aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc
     3361 cgttgacgaa gccgttagaa aacgcagcgt tttgataagc ctcagtcgcc gtgctgatag
     3421 aacccgccgg ccagacaatc gcggcaacag acaggatgac cagcgcgata attttcagcg
     3481 gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa
     3541 cgatagcgaa atagaccagg ctgtagataa acagcggcag cgcggaatca cccgtcagcg
     3601 gcgcaatccc cacttcaaaa gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca
     3661 ccgccaggta acaaaccgtt gccagcagta cgccagcgac tttaccgatt ggcgtgctga
     3721 ggctgtcaac accgccgcca acttttgcca gcgccactac cgttaatacc ggcaggccaa
     3781 cggcagtaat gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga
     3841 ccattggagg gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca
     3901 gagcgatgat atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg
     3961 gtgttgtaac gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac
     4021 aattttagcg ggaaatactc ccaaccaggc gcggcgaaga atagtttttc gatttaccgc
     4081 gttaatgcag tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa
     4141 gcaaggaggg atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa
     4201 acactatttc cgtaaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt
     4261 aaaatctaac gcccgtattt tgactgcaaa gaaaataagc cagcatcggc tggcttatgg
     4321 caataaaaga tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata
     4381 acaaaactga aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga
     4441 tgattcaccg catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa
     4501 cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg
     4561 cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg
     4621 gtgatatgcg tgccttccgg cgtatgattc acggcgttat agaccaggtt cgaaatcgca
     4681 ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt tatctatctc aaaggtaaat
     4741 gtctgttttt tctgactcag agtctgagcc tcgcgctcaa caacgcgcag catcatcggc
     4801 acatcaacct tttcattgag caattgcgtc ggtgcggctt caattttcga cagcgttagc
     4861 aactgcttca ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt
     4921 tcgcgtaccg cgccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg
     4981 gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc
     5041 agttgatgca tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc
     5101 atgacgcgaa tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa
     5161 tcacgcgttt tcaaatattg cgtaaactcc gggtaacgca gtaggttaag gatgttctgc
     5221 ccgttatctt ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa
     5281 ataccgccct cttccgtggt cagcaccacc gcgtcgggca gcgactccgc gccgctacga
     5341 aagcgtttaa tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg
     5401 tgtaagccgt atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactgcga
     5461 tccacccaca gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtcct
     5521 gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc
     5581 gggaggcagc aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg
     5641 taagatactc cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa
     5701 aagcgggttg aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg
     5761 ccgggctcca gtgctttacg caggcgacga atgtggacat cgaccgtgcg gtcttccaca
     5821 tacacgttag ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga
     5881 tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg
     5941 cccgccatca ctcggtgaga tgtcgggtcg agacttaatc cctgcatctc aatcacctct
     6001 tccaccgcca ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc
     6061 ggcgaaaacg gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct
     6121 tcttcttccc ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc
     6181 ttgaggtgtt tgatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt
     6241 aaatccggcc agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc
     6301 ggctgaaagc cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct
     6361 tctacgacca gaatacgtct cgccatgatt tgccctgttg taataaatag gttgcgatca
     6421 ttaatgcgac gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat
     6481 atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta
     6541 taatcgcgcc catgcttttt cgccagggaa ccgttatgcg catccttcac acctcagact
     6601 ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg
     6661 actggctgct ggagacagca caaacccatc aggtggatgc gattattgtt gctggtgatg
     6721 ttttcgatac cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca
     6781 atttacagca aactggctgt cacctggtgg tactggcagg aaaccatgac tcggtcgcca
     6841 cgctgaatga atcgcgcgat atcatggcgt tcctcaatac taccgtggtc gccagcgccg
     6901 gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca
     6961 ttccgttttt acgtccgcgt gacattatta ccagccaggc ggggcttaac ggtattgaaa
     7021 aacagcagca tttactggca gcgattaccg attattacca acaacactat gccgatgcct
     7081 gcaaactgcg cggcgatcag cctctgccca tcatcgccac gggacattta acgaccgtgg
     7141 gggccagtaa aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccgg
     7201 cacaaaactt tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta
     7261 ttggcggcat ggaacatgtt cgctattgcg gctcccccat tccactgagt tttgatgaat
     7321 gcggtaagag taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg
     7381 aaaacctgaa cgtaccggta acgcaaccca tggcagtgct gaaaggcgat ctggcgtcga
     7441 ttaccgcaca gctggaacag tggcgcgatg tatcgcagga gccacctgtc tggctggata
     7501 tcgaaatcac tactgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg
     7561 aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt
     7621 tagccagcca acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc
     7681 gtctggcact ggaagaactg gatgaatcgc agcagcaacg tctgcagcat cttttcacca
     7741 cgacgttgca taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa
     7801 aaacctgaac tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag
     7861 caacgggctg tttgctatta ccggcccaac aggtgcgggg aaaaccaccc tgctggacgc
     7921 catttgtctg gcgctgtatc acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa
     7981 tgatctcatg acccgcgata ccgccgaatg tctggcggag gtggagtttg aagtgaaagg
     8041 tgaagcgtac cgtgcattct ggagccagaa tcgggcgcgt aaccaacccg acggtaattt
     8101 gcaggtgcca cgcgtagagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt
     8161 gaaagataag ctggaactga cagcgacgtt aaccgggctg gattacgggc gcttcacccg
     8221 ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg
     8281 cgcggaattg ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt
     8341 ttttgagcag cacaaatcgg cccgcacaga gctggagaag ctgcaagcgc aggccagcgg
     8401 cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac
     8461 tgacgaagaa aaacagttac ttaccgcgca gcagcaagaa caacaatcac taaactggtt
     8521 aacgcgtctg gacgaattgc agcaagaagc cagccgccgt cagcaggcct tgcaacaggc
     8581 gttagccgaa gaagaaaaag cgcaacctca actggcggcg ctcagtctgg cacaaccggc
     8641 acgaaatctt cgtccgcact gggaacgcat cgcagaacac agcgcggcgc tggcgcatac
     8701 tcgccagcag attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag
     8761 cattcgccac cacgcggcga atcagtcagc agaattacag cagcagcaac aaagcctgaa
     8821 tacctggtta caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg
     8881 tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt
     8941 aacccatgct gagcaaaaac ttaatacgct tgcggcgatc acgttgacgt taaccgccga
     9001 tgaagttgct agcgccctgg cgcaacatgc cgagcaacgc ccactgcgtc agcgcctggt
     9061 cgcgctgcat gggcagattg ttccccaaca aaaacgtctg gcgcagttac aggtcgctat
     9121 ccagaatgtc acgcaagaac agacgcaacg taacgccgca cttaacgaaa tgcgccagcg
     9181 ttataaagaa aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg
     9241 catcaaaacg ctggaagctc aacgtgcaca gttacaggcg ggtcagcctt gtccactttg
     9301 tggttccacc agccacccgg cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca
     9361 gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct
     9421 gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag
     9481 cctccgacaa gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa
     9541 tatcaccttg cagccacagg acgatattca accgtggctg gatgcacaag atgagcacga
     9601 acgccagctg cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa
     9661 tcagcaaatt atccagtatc aacagcaaat tgaacaacgc cagcagcagc tcttaacggc
     9721 attggcgggt tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac
     9781 acgtcagcaa gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa
     9841 ccgtattcag cagctgacgc cgattctgga aacgttgccg caaagtgatg atctcccgca
     9901 cagcgaagaa actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt
     9961 acacagccag cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa
    10021 agcccaggcg cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt
    10081 ccttgcggcg ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct
    10141 ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca
    10201 gcatcaacaa caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca
    10261 agagttagcg caaactcacc aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg
    10321 ccagcagctg aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat
    10381 tgctcaaatg acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa
    10441 agagggcgat aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct
    10501 cgctaatcag caacttaccc ggctgcacgg gcgctatctg ttacagcgca aagccagcga
    10561 ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac
    10621 cctttccggc ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct
    10681 ggtcagccat aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga
    10741 tagcgaaacg ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac
    10801 catcggtgtg attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt
    10861 gaaaaagatc aacggcctgg gctacagcaa actggaaagt acgtttgcag tgaaataact
    10921 attcagcagg ataatgaata cagaggggcg aattatctct tggccttgct ggtcgttatc
    10981 ctgcaagcta tcactttatt ggctacggtg attggtagcc gttctggtgg ttgtgatggt
    11041 ggtatgaaaa aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa
    11101 tttggcatta tgggcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct
    11161 gccgggcata tgatctcgta ttatgcactg ggggtggtgg tcggtgcgcc aatcatcgca
    11221 ctcttttcca gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc
    11281 attggcaacg ccatgttcac gctctcttcg tcttacctga tgctcgccat tggtcggctg
    11341 gtatccggct ttccgcatgg cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt
    11401 atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc
    11461 aatttgctgg gcattccgct gggaacgtat ttaagtcagg aatttagctg gcgttacacc
    11521 tttttattga tcgctgtttt taatattgcg gtgatggcat cggtctattt ttgggtgcca
    11581 gatattcgcg acgaggcgaa aggaaagctg cgcgaacaat ttcacttttt gcgcagcccg
    11641 gccccgtggt taattttcgc cgccacgatg tttggcaacg caggtgtgtt tgcctggttc
    11701 agctacgtaa agccatacat gatgtttatt tccggttttt cggaaacggc gatgaccttt
    11761 attatgatgt tagttgggct agggatggtg ctgggaaata tgctaagtgg caggatttca
    11821 ggacgttatt caccactgcg cattgcagca gtgactgact ttataattgt actggcactg
    11881 ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc ttatttttgc ttttatttgt
    11941 tgcgcgggat tatttgccct ttcagcacct ctgcaaatat tgttactgca aaatgccaaa
    12001 ggcggagagt tattaggtgc cgcaggtggg caaatagcgt ttaacctcgg tagcgccgtc
    12061 ggcgcatatt gcggaggtat gatgctgacg ctggggctgg catataatta cgtggcgctg
    12121 cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc tgctgtatgg tcgctataag
    12181 cgccagcaag cggcggatag tccggtgctg gcgaaaccac tggggtagat tactgtcacg
    12241 gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta tccgccctac gcggttctgg
    12301 cacattttgt aggcctgata agacgcggca agcgtcgcat caggcatgat gcgccaattg
    12361 cctacgtttt ttactcttgt ggccataacc acgcagcgcc gcgtacgccg ctggaatcac
    12421 cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc gaagacaaat tgtttaatca
    12481 actgcccaac cgtttgatat aaacggtcta cattgctcat cccgcccccc aggacaatca
    12541 catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc agctcgtagc
    12601 gacgcaatgc cagttccgct accggatcgc tttcttcaac caggcggata atttcactgc
    12661 ctttcagcgc atgtccgctc aaacgacgat aatccatcgc gaatcccgtg cccgaaataa
    12721 aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga taacgcagtt
    12781 cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg ccattgccgc
    12841 cgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg ataatcacgg
    12901 caaataccgt ctgcgctccc gctgccgcgc catctactgc ttctgaaacc gccagacagt
    12961 tagcgtcatt tgccagccgc acttcccgct gcaacctcgc gcttaagtct ttatcgaatg
    13021 gctgaccgtt gagccaggtt gaattggcat tcttcaccac acgggtgtaa ggcgaaattg
    13081 agccaggaat gcccatacct accgttccgc gctgccctgt cgcctgctcc gccatatcaa
    13141 ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg ggcagacgat
    13201 ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg gtgccgccta
    13261 aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata gcgtagagac
    13321 ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc gctaaattta
    13381 acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt taccgtctta
    13441 gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg atggcattta
    13501 ccccatgcgg cagccaggac atggcgaaga tgggctgggt tcctccgatg ggatcgcaca
    13561 gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc aaagaagaaa
    13621 aaatcctccc gtctccggtg attaaacagg cgctggaagc gaaaatcgcc aaactggaag
    13681 cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac gaagtgctgc
    13741 actctctgtt gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg atcgacacgg
    13801 ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat acgctggcac
    13861 tgctgcgtaa aagcctgggg tcgttacctg tggtaccatt gagcatggaa aacccgattg
    13921 aactgacgct gaccgaatgg gttcgctccg gtagcgcggc acagggcttc cagctgcttg
    13981 atgaagccga gctgaaatcg ttgctggaag atggcggcgt gatccgcgcg aagaaacaag
    14041 atctgaccag cgaagagatc accaatcaca tcgaagccgg aaaagtggtg actaaactgg
    14101 cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg ctcaagcgtc
    14161 tgaagttttg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa gatttcgccc
    14221 agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta attcaaaacc
    14281 tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt taatgccgga
    14341 tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccagggatat aattattctc
    14401 tacggcgggc aataattttt agcgcagcaa tattatgcgt tttatgctgt aacttgctcc
    14461 atggacgttg tgtcattgtt tttcctcaag ccagtgacgc gcttcactgc gaattttccg
    14521 cattgccccg gttttcacta aatcccgtaa cagcgaaaga tcgggttgtt cgacaaataa
    14581 gagtaatgat ggatcctctt ttaaccacgt cgttttcaca tcggcagcga gctgtggatt
    14641 attcatagct agcgaccgat gccgagactc ggtcaatgtc gttaacaacg acgccggggt
    14701 atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg
    14761 ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaacaa tatctggaga
    14821 tcttcactga tatttatatc aaccttgata aattatgaat atatcggaaa ttgaaaaaat
    14881 cgcagcaaag gaaatattat tatgggttaa agaaaggata tttcagaaaa atgccctccc
    14941 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
    15001 ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacctg
    15061 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
    15121 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
    15181 agtgctgctg ctggaaaagc cgattgattt cactttgccg gaaaagtact cattactttg
    15241 aagcataaac tggccctgtt tggtagttga acatctactt cactataggg gccagaggcg
    15301 ctgactgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
    15361 gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
    15421 cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccggcatca
    15481 atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg cgatttctgc
    15541 aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac gataaccccc
    15601 acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga cggctcaaga
    15661 aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa taaaatgacg
    15721 tcataaccct gattatcgag cacttcaacc acaccttgca ggtctcgctc cactttacga
    15781 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
    15841 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag gctatgatgg
    15901 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
    15961 atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta cttttctttc
    16021 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
    16081 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
    16141 atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg aaaaatgtga
    16201 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggttactctt
    16261 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgaaccgtc ggatgttcac
    16321 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
    16381 ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
    16441 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcta aaatcaacaa
    16501 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
    16561 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
    16621 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
    16681 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
    16741 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
    16801 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
    16861 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
    16921 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
    16981 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
    17041 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat accatcaaac tagtacaatt
    17101 tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc ttactatacc
    17161 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
    17221 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
    17281 tatctggtcc gccgttctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
    17341 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcggatta agcgccgctg
    17401 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
    17461 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
    17521 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
    17581 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
    17641 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
    17701 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
    17761 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
    17821 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
    17881 tataccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
    17941 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgccgccgt taaacctggc
    18001 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
    18061 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
    18121 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
    18181 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
    18241 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagtggttct
    18301 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
    18361 atgccacgcg cccaggcgta taaatttgcc gctcaggcag taatgggttc cgcaaaaatg
    18421 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
    18481 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
    18541 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
    18601 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
    18661 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
    18721 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg tttagccctt
    18781 cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattactg
    18841 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
    18901 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta tcgttgatgc
    18961 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
    19021 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
    19081 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
    19141 ggtagctgac ccagccaaac agcagaggat aaacgacaat aatgggaagg gagagccacc
    19201 attccagcgg cgcactattg aacgacacgg taatgcccgt aagctcgagg gtgacaaggc
    19261 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
    19321 aattcagaca cataatcatc aacatagcgg tggaaggcag cacgtttacg cccattacgc
    19381 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
    19441 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
    19501 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
    19561 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
    19621 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
    19681 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
    19741 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact ttcgtagctc
    19801 acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt
    19861 acgatcatca cctttcagag attgttgcgt tgcttgatta ttacattcgc gcatcttttg
    19921 ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact
    19981 catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga
    20041 ggtcatacgc tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa
    20101 aaccagacca aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat
    20161 gttttaacca tgagcgtttg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa
    20221 aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc cccagagcgg
    20281 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg
    20341 ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcagagt
    20401 tcccgtaact catcaccatc actgcgccat ctttagtatt tagcgcctga gtgaggcccg
    20461 gcgctttggt atccggcgca acaacctggc tggcgtgggc gtgatcagcg gtgactatga
    20521 ccagcgtgtt accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat
    20581 cgaccgtctc gccaatttgc ccacaaggat tcgcagcgtg atcctgttta tcgattgacg
    20641 caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt
    20701 cggtcatctg tgccagggtt ggtacactgt cattacgttg cggattaggc gtacaggtga
    20761 ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg ccctagccag cgcactggca
    20821 tattgccgtc agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat
    20881 tcagtgaggc agcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
    20941 tttttccctg ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa
    21001 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
    21061 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgaag
    21121 tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
    21181 cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
    21241 tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg
    21301 cagccgagtc ggtgacgtag tccggtttgc cagttttttt attcagcgca tagtgagtgt
    21361 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
    21421 aattacgtgc ggctgtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
    21481 tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca cccgttaaac
    21541 ggcgagcacc gccggatgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
    21601 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cgataagagt gccagtgcaa
    21661 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
    21721 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
    21781 tgacgaggat gcttcaataa cttctttacg taatcgcgca gcagctccgt atcgtcgtca
    21841 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaagcgc
    21901 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
    21961 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
    22021 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taaca
//
LOCUS       DAZFAH010000063        22034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFAH010000063 DAZFAH010000000
VERSION     DAZFAH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.063"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..838
                     /locus_tag="ACHJWB_002247"
     CDS             155..838
                     /locus_tag="ACHJWB_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4313884.1"
                     /translation="MNLKKIFFSAVTVSALCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            882..5732
                     /gene="ehaG"
                     /locus_tag="ACHJWB_002248"
     CDS             882..5732
                     /gene="ehaG"
                     /locus_tag="ACHJWB_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4313885.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVELDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6100..7755
                     /gene="lldP"
                     /locus_tag="ACHJWB_002249"
     CDS             6100..7755
                     /gene="lldP"
                     /locus_tag="ACHJWB_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4313886.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7755..8531
                     /gene="lldR"
                     /locus_tag="ACHJWB_002250"
     CDS             7755..8531
                     /gene="lldR"
                     /locus_tag="ACHJWB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4313887.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            8528..9718
                     /gene="lldD"
                     /locus_tag="ACHJWB_002251"
     CDS             8528..9718
                     /gene="lldD"
                     /locus_tag="ACHJWB_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4313888.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9904..10377
                     /gene="trmL"
                     /locus_tag="ACHJWB_002252"
     CDS             9904..10377
                     /gene="trmL"
                     /locus_tag="ACHJWB_002252"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4313889.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(10439..11260)
                     /gene="cysE"
                     /locus_tag="ACHJWB_002253"
     CDS             complement(10439..11260)
                     /gene="cysE"
                     /locus_tag="ACHJWB_002253"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4313890.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11340..12359)
                     /gene="gpsA"
                     /locus_tag="ACHJWB_002254"
     CDS             complement(11340..12359)
                     /gene="gpsA"
                     /locus_tag="ACHJWB_002254"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4313891.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12359..12826)
                     /gene="secB"
                     /locus_tag="ACHJWB_002255"
     CDS             complement(12359..12826)
                     /gene="secB"
                     /locus_tag="ACHJWB_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4313892.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12889..13140)
                     /gene="grxC"
                     /locus_tag="ACHJWB_002256"
     CDS             complement(12889..13140)
                     /gene="grxC"
                     /locus_tag="ACHJWB_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4313893.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13282..13713)
                     /locus_tag="ACHJWB_002257"
     CDS             complement(13282..13713)
                     /locus_tag="ACHJWB_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4313894.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINRLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13958..15502
                     /gene="gpmM"
                     /locus_tag="ACHJWB_002258"
                     /gene_synonym="pgmI"
     CDS             13958..15502
                     /gene="gpmM"
                     /locus_tag="ACHJWB_002258"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4313895.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15536..16795
                     /gene="envC"
                     /locus_tag="ACHJWB_002259"
     CDS             15536..16795
                     /gene="envC"
                     /locus_tag="ACHJWB_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4313896.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16799..17758
                     /locus_tag="ACHJWB_002260"
     CDS             16799..17758
                     /locus_tag="ACHJWB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4313897.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17745..18779)
                     /gene="waaH"
                     /locus_tag="ACHJWB_002261"
     CDS             complement(17745..18779)
                     /gene="waaH"
                     /locus_tag="ACHJWB_002261"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO4313898.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19018..20043)
                     /gene="tdh"
                     /locus_tag="ACHJWB_002262"
     CDS             complement(19018..20043)
                     /gene="tdh"
                     /locus_tag="ACHJWB_002262"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4313899.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFMIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20053..21249)
                     /gene="kbl"
                     /locus_tag="ACHJWB_002263"
     CDS             complement(20053..21249)
                     /gene="kbl"
                     /locus_tag="ACHJWB_002263"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4313900.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21524..>22034)
                     /locus_tag="ACHJWB_002264"
     CDS             complement(21524..>22034)
                     /locus_tag="ACHJWB_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO4313901.1"
                     /translation="YVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMYLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5610 a         5719 c         5585 g         5120 t
ORIGIN      
        1 tgaaacgaat gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc
       61 actgtcatta atgtaaagca acatagctca ccaataatac aatggacaac tctaccggca
      121 ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag
      181 tgctgtcact gtttcagctc tctgcgcatt aacgggatgt gactacattg aagaaggcaa
      241 gccagaaagt agtttactga aacaacaaga agaacataat aataaaattg tgctacttga
      301 aaaacaacaa gcacagctaa agagtcaact cgaaactatt caaaaacaac aaactggcat
      361 aataaacagt acaaagactt taactcacgt aattaaatcc gttaaagacc aacaaaatac
      421 ttttattttt accgagttta acccagcaaa aacgaagtat ttcatcctga acaacggttc
      481 ggtggcttta gcgggtcgag tgttatccat tgacgccaca gagaatggta gtgttattca
      541 tatctcactg gtcaacttat taagtacacc tatctcaaac attggtttta atgcgacatg
      601 gggtggtgaa aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa
      661 cacatcaatg aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac
      721 cctgaagggt gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa
      781 tattcagttc gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat
      841 gcagttagta cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag
      901 ttatctggaa ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg
      961 gcaagaaatc tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt
     1021 cgtcgtttgg ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta
     1081 gtggatcagc tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta
     1141 tgaacaccag tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata
     1201 gctctgccat cgggtcaaaa acccatgcga ttggcggtgc atcaatggcc tttggggtta
     1261 gtgcaatatc agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc
     1321 aatactcaat ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg
     1381 gggactcttc caaagcggaa ggagcaaacg ccattgccct gggaaatgcc actaaagcta
     1441 ctgagattat gagtattgct cttggcgaca ccgccaatgc gtcaaaagcg tattcaatgg
     1501 cgctgggagc aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg cgtagcagtg
     1561 tagctagcgg tactgacagc ctcgcatttg gcagacaatc acttgccagc gcagcgaacg
     1621 ctattgcgat aggtgctgag accgaagccg ctgaaaatgc aactgctatt ggcaataatg
     1681 cgaaggcaaa agggactaat agcatggcaa tggggttcgg aagccttgcc gataaagtca
     1741 atactatcgc attaggaaat ggcagccagg ctctggcaga taatgcaatc gccataggcc
     1801 agggcaacaa agctgatggc gtggatgcca tcgctctggg taatggtagc cagtcgagag
     1861 gcttaaacac cattgcctta ggcacagcca gtaatgcaac tggtgataag agtcttgcgc
     1921 ttggtagtaa tagcagtgcc aacggtatta actctgtcgc gctgggcgca gattccattg
     1981 cggatttaga caataccgtc tctgtcggca atagttcatt aaaacgcaag atcgttaatg
     2041 tgaaaaatgg cgcgatcaag tctgacagtt acgatgccat taatggttca cagctttatg
     2101 ccattagcga ctcggtagca aaaaggcttg gaggaggggc tgcagtagat gttgatgacg
     2161 gtactgttac agcaccaacc tacaatttaa aaaatggtag caaaaataac gtaggggctg
     2221 cgctcgctgt acttgatgaa aacaccctgc aatgggacca aaccaaaggc aaatacagcg
     2281 ctgctcatgg tactagtagc ccaactgcca gcgtaatcac cgatgttgcg gatggcacga
     2341 tttcagcctc cagtaaggat gcggttaacg gttcccaact gaaagctacc aatgacgatg
     2401 tcgaagccaa caccgccaat atcgctacta ataccagcaa cattgccacg aatacagcaa
     2461 gtattgccac caataccacc aatatcacca acctgacgga ttccgttggt gaccttcagg
     2521 ctgatgccct gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata
     2581 ccaccagcaa aatcaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg
     2641 ttaacggctc tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg
     2701 ccaataacac ttccaatatt gccactaaca ccaccaacat ctctaatctg actgagacgg
     2761 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgta ttcacggcag
     2821 ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac ctgacgactg
     2881 gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga
     2941 ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta
     3001 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg
     3061 gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt
     3121 ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg
     3181 gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac aaaatcggtg
     3241 aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt
     3301 ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta
     3361 ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt
     3421 ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg
     3481 ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta
     3541 atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact cttgacgacg
     3601 ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag
     3661 ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg
     3721 acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg
     3781 gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc attgcgaacg
     3841 ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg
     3901 atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca
     3961 tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt
     4021 ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg
     4081 ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac tatgtgcgta
     4141 ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc gctacagcta
     4201 taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag ggcagctaca
     4261 gcgacgttga tacgggtatc gccctgggta gcagctctgt ttccagccga gtgattgcca
     4321 aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac gacaccacgg
     4381 atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt caaatcatca
     4441 acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg cagaatgcga
     4501 ttggtgcggt cgcaaccacg ccgactaaat acttccacgc taattcaacg gaagaagatt
     4561 cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg aatggcgata
     4621 aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac ggcattgcca
     4681 ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat ggttctacga
     4741 ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg cagaactctg
     4801 tcggtgaatt ctcagtcggt agtgcggatg gtcaacgtca gatcactaac gtcgcagcag
     4861 gttcggctga taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat gcgcaggttt
     4921 cccagaatac ccagagcatt actaacctgg ataatcgggt aacgaatctt gattcacgcg
     4981 tcaccaatat cgaaaacggt attggcgata tcgtcaccac cggtagcacc aagtacttca
     5041 agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtc gcgattggtt
     5101 ccggctccat tgctgccgct gacaacagcg tcgctctggg tacagggtct gtggcaaccg
     5161 aagaaaatac gatctctgta ggttcctcta ctaatcaacg tcgtatcacc aacgtagctg
     5221 caggtaaaaa tgctaccgat gctgttaacg tggcacagtt gaagtcttcc gaagctggcg
     5281 gtgtacgtta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg
     5341 gcggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg
     5401 cggtgaatta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc
     5461 gaatggttga gttggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg
     5521 cttctgcaat ggcaatgacc ggtctgccgc aggcttacac tccaggtgcc agcatggcct
     5581 ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga
     5641 gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact
     5701 ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg
     5761 caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa
     5821 caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca
     5881 caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta
     5941 aaactcacat caatattgcc aacacataac atttagttaa ccattcattg tcattatccc
     6001 tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag
     6061 actcattaca cgatgtgcgt ggactccagg agacctgcaa tgaatctctg gcaacaaaac
     6121 tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt
     6181 ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta
     6241 gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc
     6301 tcggtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca
     6361 gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt
     6421 ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct
     6481 ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc
     6541 ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca
     6601 gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac
     6661 agttttgaga ttggccagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg
     6721 ttctggatca tggcgattat ggacggctgg cgcggtatca aagagacgtg gcctgcggtc
     6781 gtggttgcgg gcggctcgtt tgccatcgct cagtacctta gctccaactt cattgggccg
     6841 gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa
     6901 cgctggcaac cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca ggttgatatg
     6961 acgctggccc acaccggtta caccgcgggt caggtgttac gtgcctggac accgttcctg
     7021 ttcctgaccg ctaccgtaac actgtggagc atcccgccgt ttaaagccct gttcgcatcg
     7081 ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt
     7141 gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac
     7201 tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg
     7261 aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta
     7321 cccatctact ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg
     7381 tcatcaacac tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg
     7441 ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc
     7501 gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc
     7561 gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc
     7621 tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac
     7681 agcctgatct tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg
     7741 tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg
     7801 tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc
     7861 aactggcgat gcaactcggc gtatcacgta attcactacg cgaggcgctg gcaaaactgg
     7921 tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg
     7981 acacatggtc ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg
     8041 attacagttt cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg
     8101 cggcaatgcg cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc
     8161 taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg
     8221 aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat
     8281 cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga
     8341 ccgaacaaca tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta
     8401 aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc
     8461 aggctcgcca cgcacggatt acccgcctgc ccggtgagca ttatgagcat tcgagggaga
     8521 aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc
     8581 gccgttcctg ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa
     8641 cgtggaagat ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca tgtctgactt
     8701 aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtagcac tggcaccggt
     8761 gggtttgtgt ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag cggcggacgc
     8821 gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc
     8881 agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg
     8941 taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat
     9001 gccgacaccg ggcgcacgct accgtgatgc gcattcaggt atgagcggcc caaatgcggc
     9061 aatgcgccgc tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa
     9121 cggtcgtcca catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga
     9181 agattacatc ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga
     9241 gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga
     9301 tgcgcgcgat gcagtacgtt ttggtgctga tggaattgtg gtttctaacc acggtggccg
     9361 ccagctggac ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa
     9421 aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat
     9481 gattgcgctc ggtgccgaca ccgtacttct gggtcgtgct ttcctgtatg cgctggcaac
     9541 agcgggccag gcgggtgtag ctaacctgct aaatctgatc gagaaagaga tgaaagtggc
     9601 aatgacgctg actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg
     9661 gctgggtaaa gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc
     9721 gtttgagccc cctcacccta accctctccc tcagggagtg gggaccgttc ggcgctgtat
     9781 gtactccctc actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggtg
     9841 aggggaaaag gccgcactgc tcaccaattt tctgctatcc tgcccccgca ctaagggggc
     9901 agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg caatatcatc
     9961 cgtctttgcg ctaataccgg ctttcgtctg catatcatcg aaccgatggg atttgcctgg
    10021 gacgataagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt
    10081 catcatgact ttcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc
    10141 acgaaaggta cgcctgctca cagcgccgta aactatcagg atggcgatta tctgatgttc
    10201 ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa
    10261 attcgcattc cgatggtgcc ggacagccgc agcatgaacc tgtccaatgc ggtgtcagtt
    10321 gtggtgtatg aagcctggcg gcagttgggg tatccgggag cggtgttgag agattagtta
    10381 cgtcatgccg gataagatgc gttatgaacc gcatcacatc cggcacgacc acaggacatc
    10441 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    10501 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
    10561 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    10621 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    10681 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    10741 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    10801 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    10861 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    10921 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    10981 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    11041 cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg
    11101 acagcttgtt cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg
    11161 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    11221 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    11281 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctgggtt
    11341 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata aagtcaatgc tgcctcgcgc
    11401 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    11461 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    11521 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    11581 ttacgcgact ggttgtcggt acaggtaagc accagatcgc caagccccgc catgcccata
    11641 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    11701 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    11761 atggcaatga cgtttttcac cgcaccgcca agctgcacac cgatgaaatc cgggttgctg
    11821 taaacgcgga aacttttgcc acagtgcagc aattgttgga gatcatcggc aaaggtctgg
    11881 tcggtagagg ccaacgaaat agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    11941 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    12001 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    12061 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    12121 agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    12181 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    12241 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtgatggca
    12301 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    12361 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    12421 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    12481 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    12541 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    12601 acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt
    12661 aaacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggctgcc
    12721 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agaaatatcc ttggtataaa
    12781 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    12841 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    12901 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    12961 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    13021 cttggcggca ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag
    13081 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    13141 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    13201 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    13261 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaaa ggcaagtttt cgccagccca
    13321 gccagcgacg ccttctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagtgcgtt
    13381 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg
    13441 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaaccgat tgatagaacc
    13501 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    13561 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    13621 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    13681 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    13741 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    13801 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    13861 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    13921 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    13981 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    14041 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    14101 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    14161 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    14221 caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa
    14281 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    14341 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    14401 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    14461 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    14521 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    14581 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    14641 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    14701 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    14761 cgaaatcact cgtgcattcg taaacgcaga tttcgacggc ttcgcgcgta agaaagtggt
    14821 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    14881 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    14941 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    15001 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    15061 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    15121 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    15181 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    15241 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    15301 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    15361 tccgctgatt tatgttggtg ataagaacgt gaaagcggtt gaaggcggca aactttctga
    15421 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    15481 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    15541 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    15601 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    15661 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    15721 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    15781 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    15841 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cgcaactgga
    15901 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    15961 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    16021 cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga
    16081 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    16141 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    16201 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    16261 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    16321 cgaccgccag aaagaagcga cgcgcaaagg caccacctat aaaccgaccg aaagcgaaaa
    16381 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    16441 tcgcgggccg acgctgcatc gctatggtga acagctacag ggcgaactac gctggaaagg
    16501 aatggttatc ggtgcctctg aaggtactga agttaaagcg attgccgacg gtcgggtgat
    16561 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    16621 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    16681 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    16741 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    16801 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    16861 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    16921 ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    16981 cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc atctcccgat
    17041 ggcaccactg agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga
    17101 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    17161 caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    17221 actggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    17281 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    17341 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    17401 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    17461 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    17521 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    17581 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    17641 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    17701 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    17761 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    17821 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    17881 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
    17941 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
    18001 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
    18061 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
    18121 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
    18181 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
    18241 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
    18301 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacgccggt tgagcgaagg
    18361 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaacacca gtcagcgttg
    18421 cactgcgcca cgtcgaggtc atcctctaac gccatggtca tcagcgtttc gtacatggtg
    18481 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
    18541 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
    18601 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
    18661 ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa
    18721 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
    18781 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
    18841 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
    18901 agagcagcta tataagagat cagcgcaatt acgatatctt tattacactg acaggatttt
    18961 acatatttaa cggattatcc tcaggcaaaa gggctggaat accagccctt gttcgtgtta
    19021 atcccaactc agaattactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
    19081 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatccagac ctgactgaat
    19141 cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatac ctttaatgaa
    19201 caagccttta aagattactt tggtccagtc gatagacata tcggacggtg gaatacccag
    19261 catcgcaatg cgaccgccgt ggttcatggt gtcgagcatg gtacgaaacg ctggcggcgc
    19321 accggacatt tccagaccga cgtcaaagcc ttcggtcatg cccagttctg ccatcacatc
    19381 attaaggttt tctttagcga cgttgaccgc acgggtgata cccattttgc gcgccagttc
    19441 gagacgatat tcgttaacat cagtgatcac cacattgcgt gcaccaacgt gtttcgccac
    19501 cgccgctgcc ataataccaa tcgggcctgc accagagacc agcacatcct cgccaaccag
    19561 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
    19621 gtcggagata ttgtcgggaa ttttgaaggc gttgaacgcc ggaatcacca gatattcggc
    19681 gaagcagccc gggcggttaa cgccgacgcc gatcgtgttg cggcacagat gggtacgacc
    19741 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    19801 aatcatgaag cccttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    19861 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    19921 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    19981 cggtacagga acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    20041 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    20101 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    20161 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    20221 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    20281 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    20341 ccacagacgg tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt tgatggacgc
    20401 ggcaacaatg gccggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    20461 ccactcaacc acctctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    20521 accaagtgtg ccggtgataa tatcgacccg gcccatcaca tcgcagtatt catgggaacc
    20581 acgaccgttt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
    20641 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
    20701 cacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    20761 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
    20821 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
    20881 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    20941 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    21001 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    21061 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    21121 gtggcttcca tcagccacag tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    21181 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    21241 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatac
    21301 gtcgcgacga gatgacgcgt taacacttct gcaaaattca ggataaataa cgtgtaactt
    21361 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg ggcataatga
    21421 tgattgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
    21481 atctcagttg gtatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
    21541 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
    21601 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggggcgt ttgtaatagc
    21661 acatgacaaa aatgccctgg tcatcatcca caatgttatt atttagtgtt attttttgac
    21721 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
    21781 caataatata tacatgattg ccaatcatga aatcaaaaac ctgttgttga gatgtgacag
    21841 taagcccttt ttttatagtg aaaagataca tcttactttc atcaaatggg aaatcccaaa
    21901 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
    21961 gaggcgtttt cactaagccc atgttgatag ctttatttac aaaataggcc ttaaggttac
    22021 ataccaaaac atac
//
LOCUS       DAZFAH010000064        21890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFAH010000064 DAZFAH010000000
VERSION     DAZFAH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.064"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            131..423
                     /locus_tag="ACHJWB_002265"
                     /pseudo
     CDS             131..423
                     /locus_tag="ACHJWB_002265"
                     /inference="COORDINATES: protein motif:HMM:NF022257.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
     gene            496..660
                     /locus_tag="ACHJWB_002266"
     CDS             496..660
                     /locus_tag="ACHJWB_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO4313902.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            629..772
                     /gene="kil"
                     /locus_tag="ACHJWB_002267"
     CDS             629..772
                     /gene="kil"
                     /locus_tag="ACHJWB_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4313903.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRSSK
                     SIH"
     gene            848..1144
                     /locus_tag="ACHJWB_002268"
     CDS             848..1144
                     /locus_tag="ACHJWB_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4313904.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1150..1935
                     /gene="bet"
                     /locus_tag="ACHJWB_002269"
     CDS             1150..1935
                     /gene="bet"
                     /locus_tag="ACHJWB_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4313905.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNNETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1932..2612
                     /locus_tag="ACHJWB_002270"
     CDS             1932..2612
                     /locus_tag="ACHJWB_002270"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4313906.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALARGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2609..2791
                     /locus_tag="ACHJWB_002271"
     CDS             2609..2791
                     /locus_tag="ACHJWB_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4313907.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            2764..2955
                     /locus_tag="ACHJWB_002272"
     CDS             2764..2955
                     /locus_tag="ACHJWB_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4313908.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            3032..3247
                     /locus_tag="ACHJWB_002273"
     CDS             3032..3247
                     /locus_tag="ACHJWB_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4313909.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3346..3567
                     /locus_tag="ACHJWB_002274"
     CDS             3346..3567
                     /locus_tag="ACHJWB_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4313910.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            3564..4202
                     /locus_tag="ACHJWB_002275"
     CDS             3564..4202
                     /locus_tag="ACHJWB_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4313911.1"
                     /translation="MSEINSQALREAAEQAMHDDWGFDADLFHELVTPSIVLALLDER
                     ERNQQYIKRRDQENEDIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEA
                     SEKRNAKLQSENAYIRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLF
                     GPGELPDESEKDAQAYFNRKYAPIDEKLMELHKWFWEQSEAERAAGIRIKGE"
     gene            4204..4959
                     /locus_tag="ACHJWB_002276"
     CDS             4204..4959
                     /locus_tag="ACHJWB_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313912.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            4970..5095
                     /locus_tag="ACHJWB_002277"
                     /pseudo
     CDS             4970..5095
                     /locus_tag="ACHJWB_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            5093..5800
                     /locus_tag="ACHJWB_002278"
     CDS             5093..5800
                     /locus_tag="ACHJWB_002278"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO4313913.1"
                     /translation="MRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWAD
                     MHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEPGNSPVIPD
                     GLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSLPATPDGWI
                     SCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGKPTWDDGDGEDLYL
                     KEVTHWMQLPEPPQEVK"
     gene            5888..6130
                     /locus_tag="ACHJWB_002279"
     CDS             5888..6130
                     /locus_tag="ACHJWB_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4313914.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            6134..6268
                     /locus_tag="ACHJWB_002280"
     CDS             6134..6268
                     /locus_tag="ACHJWB_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313915.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWFHCRG
                     "
     gene            6287..6541
                     /gene="xisR"
                     /locus_tag="ACHJWB_002281"
     CDS             6287..6541
                     /gene="xisR"
                     /locus_tag="ACHJWB_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4313916.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            6575..7861
                     /locus_tag="ACHJWB_002282"
     CDS             6575..7861
                     /locus_tag="ACHJWB_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4313917.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSKAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            7882..8583
                     /gene="mlrA"
                     /locus_tag="ACHJWB_002283"
     CDS             7882..8583
                     /gene="mlrA"
                     /locus_tag="ACHJWB_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4313918.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            8643..8750
                     /gene="yohO"
                     /locus_tag="ACHJWB_002284"
     CDS             8643..8750
                     /gene="yohO"
                     /locus_tag="ACHJWB_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4313919.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(8731..9462)
                     /gene="yehW"
                     /locus_tag="ACHJWB_002285"
     CDS             complement(8731..9462)
                     /gene="yehW"
                     /locus_tag="ACHJWB_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4313920.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(9467..10393)
                     /gene="yehX"
                     /locus_tag="ACHJWB_002286"
     CDS             complement(9467..10393)
                     /gene="yehX"
                     /locus_tag="ACHJWB_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4313921.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(10386..11543)
                     /gene="yehY"
                     /locus_tag="ACHJWB_002287"
     CDS             complement(10386..11543)
                     /gene="yehY"
                     /locus_tag="ACHJWB_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4313922.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGALLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(11550..12467)
                     /gene="osmF"
                     /locus_tag="ACHJWB_002288"
     CDS             complement(11550..12467)
                     /gene="osmF"
                     /locus_tag="ACHJWB_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4313923.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(12678..14975)
                     /gene="bglX"
                     /locus_tag="ACHJWB_002289"
     CDS             complement(12678..14975)
                     /gene="bglX"
                     /locus_tag="ACHJWB_002289"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4313924.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVNELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRD
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            15171..16886
                     /gene="dld"
                     /locus_tag="ACHJWB_002290"
     CDS             15171..16886
                     /gene="dld"
                     /locus_tag="ACHJWB_002290"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4313925.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(16924..17856)
                     /gene="pbpG"
                     /locus_tag="ACHJWB_002291"
     CDS             complement(16924..17856)
                     /gene="pbpG"
                     /locus_tag="ACHJWB_002291"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4313926.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(18030..18617)
                     /gene="yohC"
                     /locus_tag="ACHJWB_002292"
     CDS             complement(18030..18617)
                     /gene="yohC"
                     /locus_tag="ACHJWB_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4313927.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            18787..19365
                     /gene="yohD"
                     /locus_tag="ACHJWB_002293"
     CDS             18787..19365
                     /gene="yohD"
                     /locus_tag="ACHJWB_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4313928.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(19495..20256)
                     /gene="yohF"
                     /locus_tag="ACHJWB_002294"
     CDS             complement(19495..20256)
                     /gene="yohF"
                     /locus_tag="ACHJWB_002294"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4313929.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(20309..21745)
                     /gene="mdtQ"
                     /locus_tag="ACHJWB_002295"
     CDS             complement(20309..21745)
                     /gene="mdtQ"
                     /locus_tag="ACHJWB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4313930.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALDGGYKR"
BASE COUNT         5592 a         5712 c         5534 g         5052 t
ORIGIN      
        1 atcttctttg ccctccaatg tgagggcgat tttttatctg tgaggatatg aacagatgtc
       61 aaacatcaaa aaatacatca ttgattacga ctggaaagca tcaatagaaa ttgaaatcga
      121 ccatgacgta atgacagagg aaaaacttca ccagattaat aatttctggt cagactctga
      181 ataccgactc aataaacacg gctctgtatt aaatgctgta ttaatcatgc tggcgcaaca
      241 tgctctgctt atagcaattt caagcgacta aatgcatatg gtgttgtgtg tgagttcgac
      301 tggaatgatg gaaatggtca ggaaggatgg ccttcaatgg atggtagcga aggaataaga
      361 attaccgata tcgatacatc aggaatattt gattcagatg atatgactat caaggccgcc
      421 tgagtgcggc tttaccgcat accaataacg cttcactcga ggcgtttttc gttatgtata
      481 aataaggagc acaccatgca atatgccatt gcagggtggc ctgttgctgg ctgcccttcc
      541 gaatctttac ttgaacgaat cacccgtaaa ttacgtgacg gatggaaacg ccttatcgac
      601 atacttaatc agccaggagt cccaaagaat ggatcaaaca cttatggcta tccagactaa
      661 attcactatc gccactttta ttggcgatga aaagatgttt cgtgaagccg tcgacgctta
      721 taaaaaatgg atattaatgc tgaaactgag atcaagcaaa agcattcact aaccccattt
      781 cctgttttcc taatcagcct ggcatttcgc gggcgatatt ttcacagcca ttttcaggag
      841 ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca
      901 ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
      961 cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag
     1021 caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
     1081 acacatccgg tacatggttg aaaccattgc tcaccatcag gttgatattg attcagaggt
     1141 ataaaacgga tgagtacagc actcgcaacg ctggctggga agctggctga acgtgtcggc
     1201 atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggc
     1261 gatgccagcg atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta cggccttaat
     1321 ccgtggacga aagaaattta cgccttccct gacaagcaga acggcatcgt tccggtggtg
     1381 ggcgttgatg gctggtcccg tatcatcaat gaaaaccagc agtttgatgg catggacttt
     1441 gagcaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc
     1501 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaacccg cgaaggcaga
     1561 gaaatcaccg ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt
     1621 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     1681 attgtcgaaa ataccgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt
     1741 aacaatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat
     1801 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgcgc atcgtcagaa
     1861 ctgacacagg ccgaagcagt gaaagctctt ggattcctga aacagaaagc cactgagcag
     1921 aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgat gtgagagctg
     1981 tcgaacaggg ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     2041 ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct
     2101 acttccacac cctgcttgct gaggtttgca ccggtgtggc tccggaagtt aacgctaagg
     2161 ctctggccag gggaaaacag tacgagaacg acgccagaac cctctttgag ttcacttccg
     2221 gcgttaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgc accgcctgct
     2281 ctcccgatgg tttatgcagt gacggtaacg gccttgagct gaaatgcccg tttacctccc
     2341 gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaaatcggct tacatggccc
     2401 aggtgcagta cagcatgtgg gtgacgcgaa aagatgcctg gtactttgcc aactatgacc
     2461 cacgaatgaa gcgtgaaggc ctgcattatg tcgtggttga gcgggatgaa aattacatgg
     2521 cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa
     2581 ttggttttgt atttggggag caatggcgat gaagcatcct cacgataata tccgcgtagg
     2641 cacgatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt
     2701 tatccaaaat ccactgaaag cccagcggct ggctgaggag ataaataata aacgaggggc
     2761 tgtatgcaca aagcatctcc tgttgagtta agaacgagca ttgagatggc acatagcctt
     2821 gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga atttcatacg
     2881 ttagccgcat ccctttcaca aaagctggaa atgatggtgg cgaaagcaga agcagatgag
     2941 agagaccagg tatgacaacc actgaatgca tttttctggc agcgggcttc atattctgtg
     3001 tgcttatgct tgccgacatg ggacttgttc aatgacacct cagcaagaaa acgcccttcg
     3061 cagtattgcc cgtcaggcta attctgaaat caaaaaagcc agacagcagt ttccggataa
     3121 aaacgtcgat gacatttgcc gtagcgtact gaagaagcac cgcgaaacgg taacgctgat
     3181 gggattcaca ccgactcatt taagtctggc gatcggcatg ttaaacggcg tctttaagga
     3241 gcgatgaaca tgaaaagcaa aatcatcagg gagctacagg ctcctttttt attattcgca
     3301 ttcaccctca agcgtattaa ccaacaattc agggattaat gaaagatggc ggacatcatt
     3361 gattcagcat cagaaattga agaattacag cgcaatacag caataaaaat gcgtcgtctg
     3421 aactaccaga ctatatccgc cactcattgt tgtgagtgtg gcgatcccat agatgaacga
     3481 agacgtctgg ccgttcaggg ttgtcggact tgtgcaagtt gccaggaaga tctggagctt
     3541 atcagtaaac agagaggttc gaagtgagcg aaattaactc tcaggcactg cgtgaagcgg
     3601 cagagcaggc aatgcatgac gactggggat ttgatgcgga ccttttccat gagctggtaa
     3661 caccatcgat tgtgctggcg ctgctggatg aacgggaaag aaaccagcaa tacatcaaac
     3721 gccgcgacca ggagaacgag gatattgctc ttacggtagg gaagctgcgt gttgagctgg
     3781 aagcagcaga gaacaacctt attgatagtg aatgccatgt tgctgaactg gaagaagcgc
     3841 tacgcgataa gcaggcgtta cttgaagcct cagaaaagcg caacgcaaaa ttacaaagcg
     3901 agaatgcata catccgcaac cggtacaaag aactggacct gttaatcggg aaaaacattc
     3961 ttgtcatgca ggctgcgatt atcgaatggc aggcaactgg cgacgctaaa agcggactgg
     4021 catggattta taacacactg tttggcccag gcgaattgcc ggacgaatct gagaaagatg
     4081 ctcaggccta ctttaatcgc aaatatgcac cgattgacga aaagcttatg gagcttcaca
     4141 agtggttttg ggaacaaagt gaagccgagc gcgccgctgg cattcgcatc aaaggagagt
     4201 gagatgattc actaccacgg tgggcctatt actcctgata cgtgcgcaat gagagcatgg
     4261 aaagggcgac atgcgtttat cagttttgcg cattcaggcc agatcaatct cgcggctgaa
     4321 tactgtcagt cgttcgcgct ggacaacggt gcattcaccg cctggaaagc agctggcaaa
     4381 aacaaaatcg actggagcga ttactacgag tttgttgctc gctggaagaa tcacccagga
     4441 ttcgattttg ccattatccc ggatgttatt gatggcggag aggaggaaaa tgatgcgctt
     4501 ctgaatgagt ggcctcacgg aaaactagct ggcgttccag tgtggcacat gaatgaaagt
     4561 gacgagcgat ttattcattt gtgcaatgag tttccgcgag tggctatcgg tagttgtggc
     4621 gactatgacg taaagcgccc aactcttgcg gtagccagaa tgaaagacct gattcgtcac
     4681 attgttgatg ggcatggtca gccggttacg aaactacatg gattgcgcat gttaaatccg
     4741 ctgatattca caaaattacc cttagccagc gcagatagta cgaacgtcgc tcgaaacatc
     4801 ggtattgata aagcctggtc tggggcttat gcacctgcaa gtaaagagac acgcgcagca
     4861 ttaatggtag aacggattga ggcacacaat agccctggtt ctcttgcgta ttgtgaacaa
     4921 cgcgaccgct ttgaaatgca attgcaacta gcactttaag gactaacaaa tgaccactat
     4981 taccaaagaa cgtattgaat tgttcattaa aaatccgctt gaaaacgggc ttactcgtgg
     5041 cgaacaaatg gaactggcac gaattgcact ggcatcactg gaacgcgaac tgattcgcca
     5101 cgagcatgcc aaatggtctg actccacatt tggctgcgtt ggccccattg gtccactgaa
     5161 acacctctca aaagaggctc tggaagccgc agccgaacca gacgatctca gcgagtgggc
     5221 tgatatgcat ttcctgttgt gggatgcaca gcgccgtgct ggcatcagcg atgctgaaat
     5281 taccgctgct atggaagata aattgaagat caacatggag cgccagtggc ctgaaccaaa
     5341 agatggtgag cctcgcttgc acattaaaga acccggcaac tctccggtaa ttccggatgg
     5401 tttatccacg gtatgcgctg aggcttatca ggttgtagga gttatggcag atgcgcttgg
     5461 tgtattcggt gatgcagcag tacagaaagt tctggataac ctgtcacagc aaaaacttgt
     5521 tcacagagat gtgctgccgt tctcgcttcc ggcaactccg gatggttgga taagctgtag
     5581 tgagcgaatg cctgaaatgg gagagcgaca atgctatgtg ttagcagctg actttaaaaa
     5641 caactaccca ccaaacatcc ccaacactca ggtcggcgta tatggcgact ggtttaatga
     5701 tggcaagccc acttgggatg acggtgatgg cgaagacctg tatctcaaag aggtaaccca
     5761 ctggatgcag ctaccagaac cgccgcagga ggtgaaatga tgaattggcc tgaagcattc
     5821 accgcagttg gagttgcaat agcggtggca tttattctgt attcgctttt ccgctgggga
     5881 taaaggaatg ttcgctctga ttcaacgtgg tcagatatac acggacagag ccggataccc
     5941 tgtggtgatt actcgcagta ctcagcactc agtgttcttt cgacgcatgg acgggcgctc
     6001 cggacgggta cgcattggtg agttcaacaa cctgttcgaa catattgacc aacaggagta
     6061 ccgcaaaatt ctggcgggca ctgagcagga aatgcgcctg aaaaaattac gcgcaatgca
     6121 acggaggtga tacatgcata cggcttttga gttctgggtt cgcaagacat tcggcaatcg
     6181 ctacgacctg acccgtgatg tcgacggctt ctactgccgt gaagttgtga aacgaatgtt
     6241 tgacgtgtgg ttccactgcc gtggatgaaa gttttatgag gttggcatgc agacaatcat
     6301 ctatcagata acccccagca aatggtgtac ggagagagtc cttattgcat caacagggct
     6361 aaagcccggc accatcgagc gggccagaag aaagtcatgg atgcagggaa aagaataccg
     6421 ccattacgct gtagaaggtg atcctgggca ttacagtgaa tgcctgtaca acatcgaaga
     6481 aattatgcga tggatcgaaa accagaaaca accaggtgcc aaaaatgcaa gttccggtta
     6541 acctgttaat gctcctggac gtctgggagg tttaatgagt aacgcatcat acccgacagg
     6601 cgttgaaaac catggaggat cactccgtat atggtttcac tataatggca aacgtgtcag
     6661 agaaaacctc ggtgttcctg acacagccaa aaaccggaag atcgctggtg aacttcgcac
     6721 ttccgtttgt tttgcaatca gaatggggag tttcgactac gccgcgcagt tccctaattc
     6781 ccctaacctg aaacactttg gtctgggaaa aagagagata accgttaagg cactttcgga
     6841 aaaatggttg gaccttaaga aaattgagat ttgtgcgaat gcacttaacc gttaccagtc
     6901 agtaattaaa aacatgttac caatgttagg tgaaaaaaaa ctggtttcat ccataacaaa
     6961 agaggattta cttttcgtaa ggagagattt gttgaccggt taccaaaagc tttctaatgg
     7021 aaagacttct tccataaaag ggcgctcagt ggtcacggta aactactata tgacaaccat
     7081 agctggaatg tttcaatttg caacagataa tggttatacc tcaggaaacc catttaacgg
     7141 tctggctccc ttaaaaaagt ccaaggtaaa accagatcct ctcacccgtg acgaatttat
     7201 tcgttttatt gaggcttgcc gtcatcaaca aacaaaaaac ctgtggattc tcgctgtata
     7261 cacgggtatt cgtcacgggg agttggtatc gctggcatgg gaagatatag accttaaagc
     7321 aaggactata accatccgta gaaattatac aaaacttggc gaattcactc caccaaaaac
     7381 cgatgcaggc accggaagga caattcatct ggttcaacca gctattgatg ctcttaaaag
     7441 caaggcggaa atgaccatgc ttggaaagca acattctgta gaggtgaagc agagggaata
     7501 tgggagaact gctgtgcata aatgcacttt tgtttttagt cctcaggtaa caaaacagca
     7561 gcagttgtcc ggacctcact acaaggttga ctccatcagg gagtcatgga caagtatctt
     7621 aaaacgcgca ggtctgagac acagaaaatc gtaccaatcc aggcatactt atgcatgctg
     7681 gtcacttgcc gcaggagcta atcctagttt tatcgcaagc cagatgggcc acacaaacgc
     7741 acaaatggta ttcaatgttt acggagcatg gatgaaagac aacaatcacg aacagataga
     7801 actccttaac aaaagactat ctgaaagtgt cccatgtatg ccccataaga aagcggggta
     7861 aaataaaaac ttgcaaaatc aattggttta cctttaatcc ctgtcacgtt acgcgcgtgg
     7921 cagaggcgtt acggattgct gaaaccgcaa cggacagacg gcggtcatcg actgttcaac
     7981 gatgccgata ttgaccgtat ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc
     8041 agcaaagtta aaatgctgct cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat
     8101 cagcaagaaa cattactgac ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg
     8161 atcaaagagc gcggtcagga ttaccccgcc cagacactca ccacacatct gtttattcct
     8221 ctgcgccgac ggcttcagtg ccaacaaccg actctccagg cgctgctggc gatcctcgac
     8281 ggcgtactga tcaactacat cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa
     8341 gatgcgctgg tggttggctg gaatattcag gataccaccc gtctgtggct ggagggctgg
     8401 attgccagtc aacaaggatg gcgcattgat gtcctcgccc actcgctcaa tcaactacgc
     8461 cctgaactat tcgaaggccg tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc
     8521 caacagcagc aactcaccag ttggcaagaa caaggccatg atattttccc actcggcatt
     8581 taatgattcg ttaacaaatg cgctttactg tacaatcctt tcgttaacat aaggagtgca
     8641 atatgcgcat agctaaaatt ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa
     8701 ttggtggcgt catgctcgca ggttatacct ttattttgcg tgctggctaa gcgcctgcac
     8761 cagcctttca aacagtcggt ctgcgatgat cgccgccagt gccaccagta acgccccctg
     8821 gatcacatac gcggtattaa atccgctaag cccgatgatg atgggcgtac ccagcgtgct
     8881 ggcccctacc gttgaggcga tcgtcgccgt accaatgttg ataatcaccg aagttcgcac
     8941 gcccgccaga atcaccggag ccgccagcgg tagctcgacc ttacgcagtc gctgacctcg
     9001 actcattccc atacctttcg caacttctgt cacgctggca tcaatcgctc ccagcccggc
     9061 aagtgtcgcc tgcaggacgg gtagcacacc ataaaggatc aaggcgataa tcgctggttg
     9121 cagaccaaac ccaatcaccg gaacggcaat cgccagcacc gcgacaggcg gaaaagtctg
     9181 cccaacggcg gcaatagttt ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac
     9241 agcaattccg gcaccagtac caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca
     9301 gaaatgagcc agtgccagag ctgcaaaact ttcttgctga taaacgggtc gtggcagttg
     9361 tgggaacaag gcagcaaaca gcggctggct gtaaggcagc cagaaaatca gcgccacaaa
     9421 cagagcaatg agccagaaca gcggatcgcg caacatcttc atacgcttac gcctcctcca
     9481 gcagatcctg aaaatgcagc gtgccgcaag gctggccctg cgtgttcacc accggcagca
     9541 cctcgcatcc ccgcgcgaca aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca
     9601 gtgcctcacc ttctgcccgt tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa
     9661 gcaggcgcac acccagttca ctacgtccaa aaaactggcg gacaaaatca ttcgccggac
     9721 gagtcagcat cgtcagcgga ttcccctgct gcaccacttc accgtgatcc atcaatacca
     9781 gatgttctgc cagccttagc gcctcatcaa tatcatgagt gaccagcaca atagtgcgcc
     9841 ccagcaaacg gtgaatgcgc gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca
     9901 gtgcgccaaa aggttcatcc atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca
     9961 ctcccacacg ttgctgctga ccaccggaaa gctgatgcgg ataacgctca cgcaaatttg
    10021 actccagccc cagtagcgcc attaattcgt cgatacgatc gtcaatccgc gcccgcgacc
    10081 atttttgcaa ttgcggcacg gtagcgatgt tttgcgccac gctccagtgg gggaacaggc
    10141 caatagattg aatggcatag cccatccggc ggcgcaactc cagtactggc agcgagcgaa
    10201 tttcttctcc ggcaaagcgg atcacgccgc tgtcatgttc caccaggcgg ttaatcattt
    10261 tcagggtggt ggatttgccg gagccagatg tgccaatcag caccgaaaaa ctcccttcct
    10321 gaaaattgag attgagatcg ttaacggctt tttgtgcgcc aaacagtttg ctgacatggc
    10381 taaattcaat cattacgttt caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg
    10441 gtaagcaccg ccagaacaat taccgggatc acccccagca acactaaatc aatggcgctg
    10501 cttagcagcc cctggaaaac cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc
    10561 accgccatac ctacagtttg caccatcacc acccgcaggc tgcgcagaaa taccggtaac
    10621 gccagtggta actgaacatg caggaatcgc tgcgccccgc tcatgcccat cgctctggcg
    10681 ctctccagca catcgcgcgg gatctggttc aaaccgacta ccacgccgcg caccagcggc
    10741 agcaaggcat agagcaccag cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg
    10801 agcttcccca gccacggaaa ggccgtcacc agcgcggcaa gcggcgcaat caacaggcca
    10861 aagagcgcca ccgaaggcac ggtctgaatg acattgagca gagaaaaaat tgccccctgc
    10921 cgagcagtgg aaaagtagca ccagatgccc aacggcacac caatcactaa cgcaggcagc
    10981 agcgcaccaa acagcaacgt cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg
    11041 ttggcgtatt ctttcattag tgagagatcg ttaagcgtgc cggagtacag caaccacagc
    11101 ggaataatgg caatctgcat atgcaacaac cagcgccaca gcggatgcgt ggagattcgg
    11161 cggatggcat cgctacaggc cagcaatgcc agcgccgcag ccagccagaa accactgccg
    11221 aggctggtac gcgccagcgc actgccattt tgcgccagtt gtgtcgccgc ctttcccgcg
    11281 ccccacacca gcaatacgaa gacgaattgc gccagaatga gtgcacaaat gctgcctttt
    11341 ttaccgggaa taaaacaggc cgtcagccag gcgcaaccaa cgcccaccag catccagatc
    11401 gtttgcggcc acagctgcca gagatgacgc ccctcacccg aaactaaacg attaggcgcg
    11461 taactgataa acggcagcgc cgctgcgatt gccgtcagca acagcagcag cgccagaaca
    11521 ggattaatac ggaaataagt cacgggaaat tacttcgtcc accctttttg tttcaggtag
    11581 tcggcagcca cttttttggc atccagtcct tccacagcaa tgctggcatt cagttgctgc
    11641 aatgtttttg catcgaggct ggcgaagact ggctgtagcc actgtgccat ttgcggatac
    11701 tccttcaaca ccgactcacg caccactggt gcaggcgcgt agataggttg cacaccttgc
    11761 ggatcgctta aggtttgcag ccccagcgcc gctaccgggc cgtcagtgcc gtaagccatt
    11821 gcagcattaa cgccagaggt ttgctgggca gcggctttga tcgtcaccgc cgtgtcgcca
    11881 ccagccagtg acagcaactg atcctgaccg agcttaaagc cataggcttt ttcaaacgcg
    11941 ggtaacgcat cggcgcgttc gataaactct gccgaggctg ccagtttgaa ggtgccgccc
    12001 tctttcagat aacgactcag gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc
    12061 tgacgcacgg cgatagtcca ggtgttattt gcaggcgcgg gggtcagcca gattaacttg
    12121 ttgtgctccg aatcgagctt tttgactttc tcgtaacctt gctgcgcgtt tttccacgct
    12181 gcatcgtttt catctttaaa gaagaaagcg ccattgccgg tatattccgg atagatatcc
    12241 agttcaccgg aagtaatcgc cccgcgcacc acaggagtcg ttccgagttg cactttattc
    12301 accgtcggta cgccgtggct ttccagcacc tgcaaaatga tattgccgag tagcgcacct
    12361 tcggtatcga tttttgaacc gactttaacg ggggaagccg cctgtagcgg caggctcacg
    12421 gctgccaaca taaccagtga acctgcccag agctttaaga gtggcatgat gctttcctca
    12481 ttcttttact gttgttttca gcgaattaag agaaaagcat agttgataat ggcggggtta
    12541 gcttgagcgg aaggattcag ttgcagaatc agataaatac ttaagaggca tattcggtcc
    12601 ggcgtttcct gccggatgcg gcgcgagcgc cttatccggc ctacaaaggg cgcaaacgtc
    12661 gcgcccttac taaagcatta cagcaactca aactcgcctt tcttaacgcg tgcggaatca
    12721 gtgccgataa atacattgaa cttgccaggc tcggcgtcat atttcatctg ttgattccag
    12781 aacttcagcg cctcaatatc gatcgggaag ctgacagtct gagtttcgcc cggtttcagg
    12841 gtgattttct caaagccttt cagctgtttc acagggcgac tcatggaagc cgtcacatcc
    12901 tgcaagtaca tctgcactac cgtggcccca tcgcgcttac cggtgttcgt cacctgcacg
    12961 ctggcagtca ctttgccgtc acgcttcatg gtcggcgcag aaagtttcac atcagagacg
    13021 gtgaaagtgg tgtagctcag cccatagccg aacggataca acgccccgtt agcttcatca
    13081 aaataacgcg aagtgtattt gttcggcttg tcggcattat acgggcgacc ggtattcaga
    13141 tggctgtagt acaccgggat ctgcccgaca gaacgcggga aagacattgg cagcttgccg
    13201 gacgggttgt aatcgccaaa caatacatcg gcaattgcat taccgccttc agtccccgca
    13261 aaccaggttt ccagaatcgc atcagcctgc tgatcttctt tcaccagcgc cagcggacgc
    13321 ccgttcatca gcaccagcac cagcggttta ccggtggctt tcagcgccgc aatcaagtca
    13381 cgttggcttt gcggaatagt gatatcggtc cggctggagg cttcgtgcgc catcccctgt
    13441 gcttcaccga ctacagccac caccacatca gattgtttcg ccgtctgcac cgcttcatca
    13501 atcatctctt gcggcgaacg cggatcgact ttgaccgctt cttcatactg attcaggaaa
    13561 tcgataatgc ctttgtcact ggtaacgttc gcccctttgg catacagcac tttaccgttt
    13621 tcaccgacgg catttttaat cccggtcagt acggtcacgg attgatcggc aacaccggct
    13681 gcggaccagc tgcccatcac gtcacgttta ctgtccgcca gtggcccaac caccgcaatg
    13741 gtggccgatt ttttcagcgg taacgtttcg agacggtttt tcagcaacac caggctttcg
    13801 cgcgccactt cacgcgcttc tttacggtgc aggcggcttt cagcattggt atccaccggg
    13861 tcagactctt tcggccccaa atggctgtat gggtcgttaa acaatcccat atcatattta
    13921 acgttcagta catggcgggc agcatcgtcc agctcttcca tcgtcacttt gccggatttg
    13981 atcaacccag gcagatactt cgagtaatac tcgtcgctca tactcatgtt gattccggat
    14041 ttcagcgcca cgcgcaccgc atcttccggg tctgccgccg tgccatgttt aatcagctct
    14101 ttgattgcac cgtgatcgga aacggtgatg cctttaaagc cccactggtc gcgcagaaca
    14161 tctttcagca gccaggaatc ggaggtggct ggcgtgccgt tcagcgagtt cagcgccacc
    14221 atcaccgcgc cgctgcctgc gtccagcccc gctttgtacg gcggcatata atcattaaac
    14281 aggcgctgcg gactcatatc gacggtgttg tactctttac cgccttctac cgcgccgtat
    14341 gcggcaaagt gtttgacgct ggtcatcacc gagtagcgat ctgccgggct tttaccctgc
    14401 atcgcttcca ccatggtttt acccattgtt gaggtgagat acgtatcttc gccaaaacct
    14461 tcggaagcac gtccccagcg cggatcgcgc gagacatcga ccatcggtgc ccaggtcata
    14521 ttcaggccat catctgccgc ttcataagca gagacacgtc cgaccgtttt cactgcatcg
    14581 aggttaaaag acgaggccag accgaggcta atcgggaaca ccgtgcgctg accgtgcagc
    14641 acgtcgtaag caaagaaaag aggaattttc aggcggctta attccatcac ctgatcctgc
    14701 atggcgcgga tatcctgacg ggttacggtg ttgaaaatcg ccccaacctg accgtctttg
    14761 atcatctcgc ggatcgcctc tttcgggtta tccgggccga cgctgattaa gcgcagctga
    14821 ccaattttct catcaactgt cattttctta agcagttcgt tgacgaacgc atcccgcgct
    14881 tcgggcgtta atggatggtt gccgaataaa tcatccgcca gtgctggctg cagggccaga
    14941 ctcaccgcga ttcctactga acatagccat ttcatatgga tttttcttcc tctgttgccc
    15001 gacgaggcag cgaaaatggt gcaaaaaccg tagtttgcca taagcatgat ggagagagaa
    15061 aaagaatgct cagtttattg tctgaatttt caaaatattc actcgctgaa ttgtcataca
    15121 aggcgctatt ctagtttgtg atattttttc gccaccacaa ggagtggaaa atgtcttcca
    15181 tgacaacaac tgataataaa gcctttttga atgaacttgc ccgtctggtc ggtcattcac
    15241 acctgctcac cgatcccgca aaaacggccc gctatcgcaa gggcttccgt tctggtcagg
    15301 gcgacgcgct ggctgtcgtt ttccctggct cactactgga attgtggcgg gtgctgaaag
    15361 cctgcgtcac cgccgacaaa attattctga tgcaggctgc caatacaggc ctgaccgaag
    15421 gatcgacgcc aaacggtaac gactatgatc gagatgtcgt tatcatcagc accctgcgtc
    15481 tcgacaagct gcacgttctt ggcaagggcg aacaggtgct agcctatccg ggcaccacgc
    15541 tctattcgct ggaaaaagcc ctcaaaccgc tgggacgcga accgcactcg gtgattggat
    15601 cgtcgtgtat aggcgcatcg gttatcggcg gtatttgtaa caactccggc ggctcgctgg
    15661 tgcaacgtgg cccggcgtat accgaaatgt cgttattcgc gcgtataaat gaagacggca
    15721 aactgacgct ggtgaaccat ctggggattg atctgggcga aacgccggag cagatcctta
    15781 gcaagctgga tgatgatcgc atcaaagatg acgatgtgcg tcacgatggt cgtcacgccc
    15841 acgattatga ctatgtccac cgcgttcgtg atattgaagc cgacacgccc gcacgttata
    15901 acgccgatcc ggatcggtta tttgaatctt ctggttgcgc cgggaagctg gccgtctttg
    15961 cggtacgtct agataccttc gaagcggaaa aaaatcagca ggtgttttat atcggcacca
    16021 accagccgga agtgctgacc gaaatccgcc gccatattct ggctaacttt gaaaatctgc
    16081 cggttgccgg ggaatatatg caccgggata tctacgatat tgcggaaaaa tacggcaaag
    16141 acaccttcct gatgattgat aagttaggca ccgacaagat gccgttcttc tttaatctca
    16201 agggacgcac cgatgcgatg ctggagaaag tgaaattctt ccgtccgcat tttactgacc
    16261 gtgcgatgca aaaattcggt cacctgttcc ccagccattt accgccgcgc atgaaaaact
    16321 ggcgcgataa atacgagcat catctgctgt taaaaatggc gggcgatggc gtcggtgaag
    16381 ccaaatcgtg gctggtagat tatttcaaac aggccgaagg cgatttcttt gtctgtacgc
    16441 cggaggaagg cagcaaagcg tttttacacc gtttcgccgc tgcgggcgca gcaattcgtt
    16501 atcaggcggt gcattccgat gaagtcgaag acattctggc gttggatatc gctctgcggc
    16561 gtaacgacac cgagtggtat gagcatttac cgccggagat cgacagccag ctggtgcaca
    16621 agctctatta cggccatttt atgtgctatg tcttccatca ggattacata gtgaaaaaag
    16681 gcgtggatgt gcatgcgtta aaagaacaga tgctggaact gctacagcag cgcggcgcgc
    16741 agtaccctgc cgagcataac gtcggtcatt tgtataaagc accggagacg ttgcagaagt
    16801 tctatcgcga gaacgatccg accaacagca tgaatccggg gatcggtaaa accagtaaac
    16861 ggaaaaactg gcaggaagtg gagtaaaaat tacggatggc agagtatcgc catccgaatt
    16921 cacttaatcg ttctgtgccg tctgccccgc cgccgccatt tgggcggctt tttgtttttt
    16981 atagctcaac gctgctgccg gcacaggcat cactttaccg gtttcaatcc aggtacgcag
    17041 gcggctggca tcggcaaaat gggtatattt gccaaacgcg tccatcacta ccagcgccac
    17101 cggtttatta ttgataaccg tacgcatcac cagacaatgg cccgccgcat tggtaaagcc
    17161 ggttttggtt aactgaatat tccagttatc gcgatacacc agatgattag tattgcggaa
    17221 cggcagcgta tacgtcgggt tagagaaggt tgccatatct tcccgggtag tacttaactg
    17281 cccgatcaac ggatattgtt tgctggcaat gagcagtttg gttaagtcac gggcagttga
    17341 aacgttatgc accgacaatc cggtaggttc aacaaagcgc gtgttgttca ttccgagcga
    17401 tttcgctttc gcattcattg ccttaataaa ggctttgtaa ccaccgggat aatggtgcgc
    17461 aaggcttgcc gccgcgcggt tttctgaaga catcagcgcc agcaacagca tatctttacg
    17521 gctgatttcg ctattcagtc gtacgcgcga atagacccct ttcatctccg gcgtctggct
    17581 gatatccact tttagttttt catccagcgg cagtcgtgca tccagcacaa ccatcgcggt
    17641 cattaatttg ctgatagacg caatcggacg caccagatcc gggtggttcg aatagatcac
    17701 tttgttggta ttcagatcaa caatcatcgc gctaccggag gcaatttccg gttgtgaagc
    17761 ggtggtagcg gctgccgttt tcgcaacggc ctgcggtgcc aaaggcacag ccagcatcag
    17821 ggccaggcta aataaagaaa ctcgaaattt cggcatgatg agcattcaga tagtggttca
    17881 cgcgcacggg ttgcgcaccg ccggagtaag gatttactga ggctagcgac gccatcataa
    17941 cgagcaaaaa gtgcgatcgt caaaggagaa tcgtgaggaa atgctgcatt gctgacattt
    18001 acgccagcaa tgcaacgtca aagaactttc tagaacaaac gataaccgta gccccatagt
    18061 ataacggtta gggcgagcag cacttccagt accagcacgc caatcgccag cgtcgaacta
    18121 gagaagctaa ggccttcctc tttgttgata ttcaggaagc tcggaatacc aaggtaaagc
    18181 aggtagccgg tgtaaaacag cgccaccgtg ccgaccagcg cacacaacca gaccagtgga
    18241 taaagcgcca ccagaccgct taaaaacagc ggagttgcaa cgtagcccgc gaagaccatg
    18301 cagtgcgcaa gtgacggacg ctgcggataa ttacgcgcca tccaccagat gacccgcccc
    18361 attaccgcaa ccccagccag cataacgcca taaaacagga cagccagcgc cagtccggta
    18421 aaccaggata acttcaggat agtgccatcg ccaaaattcc agccaatctg tgtagtgcca
    18481 atgaaggcgc aaatcaccgg aatcgccgcc atcagcaaaa cgtggtgggt gtaatggtga
    18541 gaaatcgttt cgttttcgcg attaatcacc tgcatttcac gatcgggatg ggaaaacagt
    18601 ccccagacat ggctcatacc gcccccttgt tgtgagttca tgaacctgac agttcaagta
    18661 taagtcagct tgtgattatt ttttgttcgc tcatgtaatt tgcaccgtct gatgtacgtc
    18721 ctttttcagc gtgaatgatt tccagggtgt atgcttaaag gcaaccaaag ggagacagac
    18781 tggcctatgg atctcaatac actcatctca caatatggtt atgccgcgct ggtgatcggt
    18841 agcctggcgg aaggtgaaac cgtgactttg ctgggaggcg ttgcggcaca tcaggggcta
    18901 ttaaagttcc cgctggtggt actttctgtg gcgcttggcg gcatgattgg cgaccaggtg
    18961 ctttatctgt gcgggcggcg gtttggcggc aagctgttac gccgtttctc gaaacatcag
    19021 gataaaattg agcgggcgca gaaacttatc caacgccatc cgtatctgtt tgtcattggt
    19081 acgcgcttta tgtatggctt tcgggtgatt ggcccgacgc tgattggtgc cagccagctg
    19141 ccgccgaaaa tctttctgcc gctgaatatt ctcggcgcat ttgcctgggc gttgattttt
    19201 accactattg gttacgctgg tggtcaggtg attgcgccgt ggttgcacaa tctcgaccag
    19261 catttgaagc actgggtctg gttgattctg gtcgtggttc tggtggtggg cgtgcgctgg
    19321 tggctgaaac ggcgcgggaa gaaaaagccg gatcatcagg cgtaaaacca ttgccctggg
    19381 taaggcgttc gcgccgcatc cgacatccgg tgcaccatgc ctgatgcgac gcttgtgcgt
    19441 cttatcatgc ctaccaaaat tttacccatt gaaataacca cgcacctcac tgcgctattc
    19501 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgcccggt
    19561 ggtgtaattt gcgccctctg agcacagcca cgctaccagg ctggcaatct catgcgttgc
    19621 gccaaaacgc cgcagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tgtcatccat
    19681 gccattcatt ggcgtggcga tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    19741 gaccagctcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    19801 agcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    19861 gccctgccct tgtttcacca tctggcgggc cgcaatttgt gagcataaaa atgcaccatc
    19921 gacatcaacg gtaaaaatct tgcgccactc atcgaaagcc atatcaagaa acggcgcttt
    19981 ggtcattgcg cccgcattat tcaccagcac atcaatacgc cccagccgtt gaatgagttt
    20041 ctccagcgcc tgcgcacctt ctggcagttt accgagatcc agctgcacga tctccgcacg
    20101 tacgccgtgg ctaactacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    20161 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    20221 cgaatcggag gcggtaataa tcgcaacctg tgccatcgag ttctccactt aacgctgaat
    20281 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgtcc aacgcaccag
    20341 tgagttgaat ggaagcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    20401 gcagcgcggg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    20461 gcgcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    20521 ttttctccgc aagtgtctga acctgactgg ctgcccgcgc cacgtcattc accgcttcaa
    20581 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcaagat
    20641 tggcgttaag acgaccacta tcgaaaatgg gtagcgtcag gcctgccgta acgcccattt
    20701 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    20761 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatggtgctt agcgatgact
    20821 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    20881 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgactttg
    20941 tctggttatt tgtaagtgcg cttaaccgtg cctcaataat tttcattttc cccgcgacat
    21001 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacacct tcaactgaag
    21061 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    21121 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    21181 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    21241 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    21301 ccgttaagcc aaaagtaccg ttggtgtacc acgggccggt cgtacctgcg gccggatcgt
    21361 tcagagcaaa cggccccatt aagccttctg ccgacatttt ttgccgctcc atatccgccg
    21421 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    21481 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    21541 aagtgagttg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    21601 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    21661 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    21721 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaaagg tgcgcggaga
    21781 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    21841 cgacatatcc agaaaaatat agactaagta aatgatatct tccgatttat
//
LOCUS       DAZFAH010000065        21692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFAH010000065 DAZFAH010000000
VERSION     DAZFAH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.065"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            329..3001
                     /gene="rcsD"
                     /locus_tag="ACHJWB_002296"
     CDS             329..3001
                     /gene="rcsD"
                     /locus_tag="ACHJWB_002296"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4313931.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            3018..3668
                     /gene="rcsB"
                     /locus_tag="ACHJWB_002297"
     CDS             3018..3668
                     /gene="rcsB"
                     /locus_tag="ACHJWB_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4313932.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3868..6717)
                     /gene="rcsC"
                     /locus_tag="ACHJWB_002298"
     CDS             complement(3868..6717)
                     /gene="rcsC"
                     /locus_tag="ACHJWB_002298"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4313933.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTHLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6992..7768)
                     /gene="yfaP"
                     /locus_tag="ACHJWB_002299"
     CDS             complement(6992..7768)
                     /gene="yfaP"
                     /locus_tag="ACHJWB_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4313934.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7773..9422)
                     /gene="yfaQ"
                     /locus_tag="ACHJWB_002300"
     CDS             complement(7773..9422)
                     /gene="yfaQ"
                     /locus_tag="ACHJWB_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO4313935.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(9423..13937)
                     /locus_tag="ACHJWB_002301"
     CDS             complement(9423..13937)
                     /locus_tag="ACHJWB_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO4313936.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSYAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDISDINDSLILDTPESPLADA
                     LANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13961..14584)
                     /gene="yfaT"
                     /locus_tag="ACHJWB_002302"
     CDS             complement(13961..14584)
                     /gene="yfaT"
                     /locus_tag="ACHJWB_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO4313937.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(14581..16269)
                     /gene="yfaA"
                     /locus_tag="ACHJWB_002303"
     CDS             complement(14581..16269)
                     /gene="yfaA"
                     /locus_tag="ACHJWB_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4313938.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(16418..19045)
                     /gene="gyrA"
                     /locus_tag="ACHJWB_002304"
     CDS             complement(16418..19045)
                     /gene="gyrA"
                     /locus_tag="ACHJWB_002304"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4313939.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            complement(19271..20473)
                     /locus_tag="ACHJWB_002305"
     CDS             complement(19271..20473)
                     /locus_tag="ACHJWB_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFO4313940.1"
                     /translation="MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDTGISGVGEVGL
                     AYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDI
                     ALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
                     AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDAD
                     IIVEIHSLPGTNSAIQFAKAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERS
                     YTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTTVQVHVCGGPVSTVA
                     ALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQ
                     QYLAYVIK"
     gene            complement(20486..>21692)
                     /locus_tag="ACHJWB_002306"
     CDS             complement(20486..>21692)
                     /locus_tag="ACHJWB_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4313941.1"
                     /translation="LRMNESLGITPEDFANISSIFFVSYLIFQIPSSIGLQKLGARKW
                     ISAIIVGWGTVTTLIFFAKDTQHILLARVFLGIFEAGFFPGMVYYLACWFPARERGKV
                     NSFFMLSIAVASVLAAPMSGWIIEHMNTGNYEGWRWLFAIEGIPTVLLGALTFYLLPD
                     RPEKARWLTPSQVTALVDKLNSDNEKAAALNKNTNASFLSIIKNPVLLQLSFAYMFIQ
                     AAALAANYWLPGLVKGFSSDFTDTDVGLIMSIPFIFAMFSMPLWGWHSDKKNERKWHA
                     ALPMLVAGCGFLMIALVPSMTLRMMGLTLYGVGILSYYGPYWALPSALLSPSGLAISI
                     AFINSCSSLGGFLINKSLGYVSTHYGATGIFIVEAILCFIAVAILLSMKIDDKKDNAQ
                     KENIISHTK"
BASE COUNT         5379 a         5795 c         5168 g         5350 t
ORIGIN      
        1 tcgcatccgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
       61 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
      121 ctttgtttat ttgggattat ttttgttggg ctttttgtag gttatttgta cagcaaaatg
      181 gcgcttgtac atctatttcc cccaatgcag gatgataaat atcacgggag aatagagaat
      241 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
      301 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
      361 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
      421 ggtgacgatg ggggtaatgg tacaaagcgc cgttaacgcc tggctgaaag ataaaagtta
      481 ccagattgtc gacattaccc acgctatcca aaagcgcgtc gatacctggc gttacgtgac
      541 ctggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
      601 agagacgcgc ctgaaacagg atgtctacta tctggaaaaa ccgcgccgca aaacggaagc
      661 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcaacgga tgtccaccta
      721 tctggacaca ttgtggggcg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
      781 ggataacagt ctggtgctga tctcaaccct gcccctcaaa gatctcacct ccggatttaa
      841 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
      901 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
      961 ttactttacc ttgcgtacta cctttaacca gccaggacat ctggcaacgg tcgtggcttt
     1021 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
     1081 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
     1141 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
     1201 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
     1261 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
     1321 ccatttctcc agccgcagta cagaaaacgt ccccagcacg gcggtcaata acgaattgcg
     1381 cattttacgg gcaatcaatg aagagatagt ctcactgctg ccgctcggcc tgctggttca
     1441 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
     1501 tttgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
     1561 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
     1621 aattttcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
     1681 gcagcgtctg tatgagaaaa accagcaggg ccggatgacc tttatgaaaa acattggcga
     1741 tgcgctgaaa gaacctgcac agtccctggc ggagagcgcg gctaaactca acgccccgga
     1801 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgatg aaatacagtt
     1861 agcgaacatg cttgcggacg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
     1921 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
     1981 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
     2041 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
     2101 ccttgaggtt gatcaggatg agtcctccga agaccgcctg acgttccgca ttctggacac
     2161 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
     2221 ccaaaacgat cgctatggca aggcggaccc gctggcattc tggctgagcg atcaactggc
     2281 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
     2341 tgtgcatatc aaaatgctcg cagctgaccc ggaagttgaa gaggaagaag agcgtttact
     2401 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
     2461 ccagttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
     2521 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
     2581 cgatgatgag tctggcgtac gggaaattgg gcctggtcaa ttgtgcgtca acttcaatat
     2641 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
     2701 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
     2761 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
     2821 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
     2881 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
     2941 tgttccagga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
     3001 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
     3061 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
     3121 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
     3181 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
     3241 agcgccattt cccaagcctg tcgatcattg ttctgaccat gaacaacaac ccggcgattc
     3301 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
     3361 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
     3421 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
     3481 agagtgaagt tctgcgcctg tttgcggaag gcttcctggt gaccgagatc gctaaaaagc
     3541 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggtg
     3601 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
     3661 aagactaatc acctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
     3721 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
     3781 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
     3841 agatgcgaca taaacacctt atccgtccta cgattcccgc gatttcctga ccctctcggc
     3901 atataccgtc agcgtctgtt ttatcacatc cagcgttacc ggcttcgaca ggcagctgtc
     3961 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
     4021 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
     4081 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttgctaagta cattaagcgc
     4141 atcgacacca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
     4201 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cattatcgct
     4261 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
     4321 ataaatacgc gccaacaatg ccggtagctc atgcggggca gccacactgt gtacccactc
     4381 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
     4441 accctgccat tttttactca ctacctcgtc agtaattaac acatcttcgg gagtcggttc
     4501 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
     4561 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
     4621 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
     4681 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
     4741 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccga cctggaagaa
     4801 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
     4861 gatagagaga taatcgccat cagcgcggac atgcaaaact atacagccgg tatcggtgaa
     4921 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
     4981 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
     5041 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
     5101 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
     5161 caacaacagg ctggaagagt tgttcattgc cgtcaccagc cgatcgacgc ctttcggtaa
     5221 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
     5281 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
     5341 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
     5401 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat gggtattgtt
     5461 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
     5521 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
     5581 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
     5641 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
     5701 tcgctcgtac atccgtgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
     5761 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
     5821 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
     5881 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
     5941 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
     6001 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
     6061 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
     6121 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
     6181 attgatgcgt tcatgcaaag ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
     6241 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
     6301 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
     6361 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
     6421 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
     6481 attttcggcg atgtacttca gctctttcat cacatcagag gtgcgttgaa taaagcgctg
     6541 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
     6601 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
     6661 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
     6721 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
     6781 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
     6841 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
     6901 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
     6961 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
     7021 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
     7081 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
     7141 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
     7201 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
     7261 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccgtat
     7321 cccacgagag aaccagccgt aaacgtgcac gaatcgttcc tgtacccggc gttgagtaaa
     7381 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
     7441 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
     7501 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
     7561 tgccgcggat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
     7621 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
     7681 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
     7741 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
     7801 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccag gtgcagatac
     7861 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
     7921 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
     7981 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
     8041 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
     8101 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctctccctg ccccgcctgg
     8161 gccgtggctt gccgccagga aagtgtccct tcggtagctc gactgccatg ataatgaacc
     8221 ggatcgcctg catagatgag atcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
     8281 gctggcgaag cagaaacacg ctgcgttgcg ctgctgtcgg ggatggtcag gcaatcgcct
     8341 tcccgattgg cgttttgctg taaaaaagtg cgaatcgcca cggtcatggc tttcgctgcc
     8401 tcagggggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
     8461 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
     8521 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgccg
     8581 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
     8641 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
     8701 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
     8761 ggtgtaccat cggttaacca gccggcaaaa ccaccctgac ggatttcctg tttatcatcg
     8821 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatgc gacaccgatt
     8881 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
     8941 gtcgctaatg aatcgagcag cgatttaacc gttaccgagg tttctggctt cagcgtagta
     9001 agagacgcta accacgcagg cgcctgacgc tgttgccagt attgccccca cacatctgcg
     9061 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
     9121 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
     9181 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
     9241 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
     9301 gacgacagct gatacagttg atcgtgctgc gcaccacgca gtgccagttg taacggcgtc
     9361 tcctccgcaa gcgttggctg agtaaccaac gccaatagcc agacgattct gcgccagttc
     9421 acttatttca cctgcatccc ggtccattca ctccctgccg caacactttg ctgtgcaggt
     9481 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
     9541 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
     9601 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgtttcgc ggcgttgggt
     9661 ttattgaccg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggtgg cagcggtact
     9721 tccacctgac cgtagcgcag aactgcatcc tgctcgctgg taagcgtgat ttcatcgaga
     9781 tacagcgcat cgctgtcaat ctcattgctg gtcaccggtt gcagaataaa gctcatctct
     9841 tcttcaccag ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
     9901 tgagccattt ttgccggctc acgccagcgg acctgcacat tttgcggcga taattcgtca
     9961 ccaaaagaga gaatgtccgg cacgccctga ccaacccaac gccagtattc gccccctcca
    10021 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    10081 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    10141 gtcaaaccac ttaaaatagc ggccgtctgg gtagcgtcac caccgctatt gagcagcaca
    10201 accgtgtgcg ccagcggctg attgctgttt gccgctttat cccacgcata ttgctgaacc
    10261 tgtggcatca ccgtggactt caactgcgct tttttcagca acgtcatggt taagacgttt
    10321 gccagcgcat ctgccagtgg agattccggt gtatcgagga tcaggctgtc attgatatcg
    10381 ctgatgtctt cctcagagaa atcttcatct ttagttccgc gccgggcgat agcttcatcc
    10441 aaccctttca acaacgtttt gcacggcaga ttcatctcct gtgcccacgc cagcaccagc
    10501 gcccgatgca ataacggcat gttatccgcc tgctcggcat aactgtcgag catatgctgc
    10561 cagtattccg gttgttgcgt tacgccgagc gcctggctgg cctgccagtc ggcgtaccat
    10621 gcccatgccg taaggaaggc gtcaccattg ccatcttcac cccaccaggt aaagcgcgct
    10681 ccgggccccg ccagttgcat cagccgcaga cggttatcct gaatcatctg acgaatgtcg
    10741 ttagcggcgg cactttgatg atcggcaagc gaacgccagg cgagactgag tgggatcaga
    10801 cggctaccgg tgttgatcac cccaccccac ggttcatcga ctaacgcatc aagattgttg
    10861 cggaaaatct cctgcggcgt ttcactactt tgtagccgga tattactcgc ctgctcgggc
    10921 aacatcagcg cgttctcgcc accgccgagc atgacatttt tctgctgttc aacgggccag
    10981 ctgttgtcca caaaagacag ttttgtgcta atgctgtcct gcacttgccc attttgttgc
    11041 agttctgcac ttaacaagcc agattgctga atattctgcg tcagcgaaat ataattcgcc
    11101 cctttgtgca gcgtcagcgt ctggcgcatc tcagcgcctg caaatttagt caccagcgcc
    11161 accggttcgt tatcctgctg actgaagata aacagtcctg ccgccggttt gtcgcccacg
    11221 cgatacaccg ttggcatact ccacttcatg tagagatttt tttccgaacg cagataagca
    11281 cgcccctgcc cgaccagccc gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt
    11341 aacgaatcag gcatcaggaa cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac
    11401 ggcatccatg ccgcggtatc cacctcttca cgccgtggac gttcaagcat ttttactcgc
    11461 cgctcgctgc ggttagttgc gccaggcgca accggctcgc tggagagtgc ctggtcgtag
    11521 ctgataaacg acagactgga gctggtgcgt acattgttac gccccagcgg atagaaaaat
    11581 ttgccgatat tcggcgcgat ttccggttgc agcgcgtaga tcatttcatc gaccacgcca
    11641 accgttagct gcgcagaaac aggtttacct ttcagcgacg aggttaactc gacattgacc
    11701 agttcaccag gctggtaatg ggttttgtcc gttttcaccc ggatatcaag ctgaggaacg
    11761 gcaactttga tcccggcgtt ctgaaaactg tactgaccat tacgggtata cagcaccgaa
    11821 aaagtgatgt taggcgcaaa ggaattgctc actggaaccc gggcttcata ctgggtatcg
    11881 tttaaacgtt gtagcgttag ccagtttgcc ggatgcgaaa gcagcgactg ctgttccacg
    11941 cgatcgcgtt ccagcgtcaa taatgcttca tcaattggct ccggaaaagt aatcagcatc
    12001 ttcgcggttt cgcctggctg gtacagcgtt ttatccgcca cgatatctac cgtacccgta
    12061 tgcgccgtgc tgcccttacc gctgacggca taacttaacc cagcgagaat taagccgtct
    12121 ttatcgcgta atgtcagatt gtagttgcca ggtttagcga aattgacggt aaaggatttg
    12181 ccgcctgacg gtagctctcc gctatggctc gtgcggtctt cgagacgcaa ccattcatac
    12241 gtaacaggaa cctgttttga agattccagc gcggcataac ggaacacaac cgactcgccg
    12301 ctattactgt attgtgcggc agtacttaat gagtaatgcg ccagaccgcg ttcaatgagg
    12361 atctctttgg tggtggtgac acgatacgcc gcgccgtcac tggcggagac ggttaacaaa
    12421 taacggctcg gtttatcggc ggcggggaga tttaacgcca catgaccgct ggcgtcggac
    12481 accgtttcgc tgccttccag cgacacgggg aaacgtccgg cataacgcaa atcgttaccg
    12541 accattgata attgctgagc gcgcaaactt aactgcacgc gggcattttt taccggctcg
    12601 ccatccgggt agagcagttg cagtttgccg ctgaccgctt cgccagtttt gaactctttt
    12661 ttgtcgagag ctaaaccaat ctcgaaatgt ggcttgatgt agtttgccac gcgaaaactg
    12721 ctgctataga cctgattgcg gtaagcaaga cgtaactcat aacctccggc aacggcattt
    12781 tctggcaggc ggaaacttcc ctgcccgcca ttgcgcgcat ccagcgtgac attgacggtt
    12841 tgcaacagac tgccgttggc gtccagcacc gaaagcttcg ccggggcgct gacgatgggg
    12901 gatgaatgca acggatcgtg gaactcgcgg cccatcactt taacatcgac acgatcgcct
    12961 gcgcgatata gcgggcgatc ggtaaagata tacaagcggg tgttgtagat ttcactttcg
    13021 taaaagaagt tctcggagac aaaaacgccg ccttcagcat ccttacccag aatgtatgag
    13081 cgttctggcg atatatgttg taactgcaag gtaccgctgt catcggtcac accgcgggtc
    13141 atcacgccaa gaccgtcagt ccacaagatc tctgagccgg gcttcgcttc accctgtttt
    13201 ttacccgcgg tccacaccag aagctcgttg cctgacactt tgctaagcgc cacggtatcg
    13261 gaaacaaaca ccaccgtcgt cgcccgatac ccaccaacca tcgcctcgac gaggtacagt
    13321 cccggctctt gttggccgag aggaatataa atgttacccg gctgcggcga gatgaaattg
    13381 ctggatgcgc cttccagttt taccccttgc tgcggctcga ccggtttagc ctgccatagt
    13441 ggataacgga actgttccac caagggatat tttttcagcg gggaaaactg gttgttctgt
    13501 acataacggg aaggtttaat aatggcattg ccgagatgta attcgggtaa tgcctgagtc
    13561 acattctgcc gtgactgaga agagaaagta cgctgcatta cgcggcgaga tttgccgtac
    13621 cagttatccc acagccaggt cagcgtattg ttcagcccgt cgcccagata ttgcggttgc
    13681 accacaatgc gatgcaggtt tttctgctgg cgcaaaaatg ccatcgggtc aggaatacga
    13741 tacaggcgaa cgtccacgcc gccgtactct tccatctgat agcgccgata atcacgcccc
    13801 ggcgcttcca gtcgcacttt cgcctcttca ctgctgctaa aactgctgtc agcaagcaaa
    13861 aagaatgttc ccccggcggg cggcgcatag ttactggaag gaagcgaatc atcagcatta
    13921 gcaagccctg ttcccacaag agacagggac aagcacgctg ctatcgcgcc agaaaattta
    13981 aacgataaat gccaatgaaa ttgggattgg aatcgttggg tatccatcgg gtgtccttcc
    14041 atgtcataag ttgttgcaga ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc
    14101 cggtgtggta gatgacgtaa cgccccatcc agaccattaa gtgctgggca tcgccctgat
    14161 cgaaaaaaat catatcgcca ggcagcgcct ggtttatgtc ctggccaata aactggctgt
    14221 tgtactgaat caaattaatc gcggtcacgt aggggccggt tttcccgttc ccctgattcc
    14281 agttttgcgc cagttgacgc tgtccaggtg ttagcgtcat ctctggcggc aaatactggc
    14341 tagagaaacc gttacttttt agccatttac tatcgtgaac tttcagcgtc tcgttcgccg
    14401 caaatcgcac caggcccgca caatcctgct gataccagcg tggacttggc ccctggcgga
    14461 gctgttcttg tgcaatgcgt acaaaccagg cgcgaaacag cccggattgt tcgacattca
    14521 gcatttcgct atgggcaaca acacaacaca gccagcaaat cagcgccagc agcccgtgcc
    14581 tcatagcggc tgccaggtta tgggtagcca ctgccaggcg gcaccgggtt ccatctgggc
    14641 cagcttcatg acataacgcg gttgttgaga taaagcgtcc agcttcggca ttaataaagt
    14701 ttgtgcggcg ttataaaaaa ccggttcgag attcttcggc agactggtca acgtttcgtt
    14761 acgcagcagt ttcgccacgc cttgtggatt gatatagagc ggaacgatgc catcagtggg
    14821 tattacatcc accattgccg ggcggttttt attcagtgtt tgcagggcgt tattaaccag
    14881 cgtgtcatcg agggagaaaa gcagcgtttt gttttgcatc gccagcgaca cgcggaaaaa
    14941 ataatccgac attaattgat cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg
    15001 ggaactcact tcgcgacgcc agatttgcgc ttcgccctgc tgagtctggc ttatcggcaa
    15061 aacaccttct ggcgctttgc tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg
    15121 caattgcgcc tgttcggcag tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt
    15181 tgagtctgca taccagcaca gccccgcggc accgtctaac gccccattca acttgtcgtt
    15241 ttcctgactg atgtgcgaaa gcatctcttc ggcaataccg tgtgaatacg gcaccaccac
    15301 acagaagctg gccccggcag gcatactgtt ccagaccggc gtaaaatcga aactggtatc
    15361 tacgctggcg gattcatcat ttaacgccac aaaactgtgc cagccgtcgt tacccatttc
    15421 gaagcgtacg ccagcaaaag aaggcattaa ccgctggtag ccaaaaccca gcaacctggc
    15481 gctgaccacg atgcgctggc gtactggcgt tttttcagca gcacgctctt ccaggccaaa
    15541 gcttgcttgc cagcgttttt tgccgctcaa caaatcacct gcgatcgccg tggcttcggt
    15601 atcctgctga tcatctttaa acaacatatc cgtgctggaa aacaccagca tcttgtcctg
    15661 ataagtcgcg aacatcaggg cgttattgcc gttatagcgc aactgataaa caggaatagt
    15721 ttcactattt atcttgatgc tactgatttc cgttttgctt aactggctgt cgctggtagc
    15781 ggcaaacaac aatggctcca gcagtttgct taaaccactg cgctggatca gcaccatgta
    15841 atgtgaaaga tggccttgtt tatcgtgcca tagcgccgcc tgcgcgggct gatctaagag
    15901 tgacgaaaag agtttatctt tcagcgtgag atcgtgttca tagacaatac gacgaatgct
    15961 gccttcaatg cccagacgat cggcatgatt ctgataatag aaaacgaaat cttcgctcag
    16021 aacatcgtgg agaaacggaa tggtgaggag atctttggga agctggctca gagagtcgct
    16081 gtcgagaaag aggtccggct cattgagatc gatttgcaga ttgttgtgca ccaccagcgg
    16141 cgacaacgtt ttttctggcc cactgccagc atattgcaac gcccagacgc cagcggaaag
    16201 cagtgctatt gcgccaaaac ctacaagacc atagaaccgc cagcctttcg cctttttttc
    16261 accactcatt gccacattcc ttgtgtatag ccagccattt tttacgggca cagccaaact
    16321 ttaccgtgcc ctaatacgac aaaagcccag actttgcagc ctggactttt caattcaaac
    16381 aagggagata gctccctttt ggcatgaaga agtaaaatta ttcttcttct ggctcgtcgt
    16441 caacgtccac ttccggagcg atttcatcgt ccccttccgc ggcactgcca tcgatggtat
    16501 ccagatcttc ctcgtcaacc ggttcagcaa cacgttgcag acccactacg ttttcatctt
    16561 ccgcagtacg gatgaggatc acgccctggg tgttacggcc tacgatgctg atttccgaaa
    16621 cgcgagtacg taccagcgta ccggcatcgg tgatcatcat gatctggtcg cagtcatcta
    16681 cctgtaccgc gccaaccact aaaccattac gttcagtaac cttaatggag ataacccctt
    16741 tcgtcgcacg cgacttggtt gggtattccg ccactgcggt acgtttaccg taaccgtttt
    16801 gcgttgcggt gaggattgcg ccatcgccac gaggcacgat cagagagacg actttatcgc
    16861 cttcacctaa gcgaataccg cgaacaccgg tggtgttgca gcccatcgca cggacagaag
    16921 actctttaaa gcgcaccact ttaccttcag cggagaacag cattacttcg tcttcgccgc
    16981 tggtcaggtc aacgccgatc agctcatcgc cgtcaaccag tttgatcgcc actttaccgg
    17041 cggtacgcag acggttgaac tcggtgagga cagttttctt cacggtaccg ttagcggtcg
    17101 ccatgaagac tttcacgcct tcttcaaact cggtcactgg caggatcgca gtgatacgtt
    17161 cgtcctgctc cagcggcagc aggttgacga tcggacgacc gcgcgcgcca cgagtggctt
    17221 ccggcaactg ataaactttc atcgaataga cgcgaccacg gctggagaag cacagaatat
    17281 ggtcgtgagt gttcgccacc agcagtcggt cgataaagtc ttcttcttta atacgtgcgg
    17341 cagatttacc tttcccgcca cgacgctgcg cttcgtattc agaaagcggc tgatacttaa
    17401 cgtagccctg gtgagagagc gtcactacca catcttcctg agtgatcaga tcttccaggt
    17461 tgatgtctgc gctgttggcg gtgatttcag tacgacgttt gtcaccgaac tgttcacgaa
    17521 ccagctccag ctcttcacgg atcacttcca tcaaacgatc ggcgctacca agaatacgca
    17581 acagttccgc gatctgatcc agcagctctt tgtattcgtc gagcagtttt tcgtgctcaa
    17641 ggccggtcag tttctgcaaa cgcagatcca gaatcgcctg agcttgctgt tcggtcaggt
    17701 agtacagacc atcacgcacg ccgaactctg gctccagcca ttccggacgc gcagcatcgt
    17761 cgccagcacg ttcgagcatc gcggcaacgt tgcccagctg ccacggatta gcaaccagcg
    17821 cagttttcgc ttctgcaggc gtcggcgcat gacggatcag ttcgatgatc gggtcgatgt
    17881 tcgccagcgc cacggctaat gcttcaagga tatgagcacg atcgcgagct ttacgcagtt
    17941 cgaaaatagt acgacgggtc accacttcac ggcggtgacg aacaaacgcc gcgatgatgt
    18001 ctttcaggtt catgatcttc ggctgaccat ggtgcaatgc caccatgttg ataccgaaag
    18061 aaacctgcaa ctgggtctgg gagtagaggt tgttgagcac aacttcaccg accgcatcgc
    18121 gtttcacttc aatcacgatg cgcataccgt ctttgtcaga ctcgtcacgc agcgcgctga
    18181 tgccttccac gcgtttttct tttaccagtt ccgcaatctt ctcgatcagg cgcgctttgt
    18241 ttacctgata cggaatttcg tggacgataa tggtttcacg accggttttg gcgtcaactt
    18301 ccacttctgc gcgagcgcgg atatacacct tgccgcgacc ggtacggtaa gcttcttcaa
    18361 taccgcgacg accgttaatg attgccgccg tcgggaagtc cggccccggg atgtgttcca
    18421 tcagcccttc aatgctgatg tcttcatcat cgatatacgc cagacaaccg ttgatgactt
    18481 ccgtcaggtt gtgcggcggg atgttggttg ccatacctac ggcgataccg gaagaaccgt
    18541 tcaccagcag gttaggaatt ttggttggca tgacgtcagg aattttttcc gtgccgtcat
    18601 agttatcaac gaaatcgacc gtctcttttt cgagatcagc catcagttca tgggcaattt
    18661 tcgccagacg gatttccgta taacgcattg ccgccgcaga gtcgccgtcg atggaaccga
    18721 agttaccctg gccgtctacc agcatgtaac gcagcgagaa tggctgcgcc atacggacga
    18781 tcgtgtcata aaccgccgag tcaccatggg gatggtattt accgattacg tcaccaacga
    18841 cacgggcaga ttttttatag gctttgttcc agtcattgcc tagtacgttc atggcgtaaa
    18901 gtacgcgacg gtgtaccggc ttcaggccat ctcggacatc tggcagtgca cggccaacaa
    18961 tgaccgacat cgcataatcc agataggagc tcttcagctc ttcctcaatg ttgaccggtg
    19021 taatttctct cgcaaggtcg ctcatctaac cgctatccct ctactgtatc ccggattcaa
    19081 aggtcggaga ttataacaca atcgcttgcg gacgggtaaa cgtaatgcag gcttccgggc
    19141 gctgtagatc tactgcgttt taccccaccg ccagcacaca tcgcgctggc ggtgaacggc
    19201 aaaggaacaa agaccgcaac acgcttcctg tgcctcaata aacaccagaa ataatggcct
    19261 taaatcgctg ttatttgatc acataggcaa gatattgttg tacgatttca tcgtttaatt
    19321 cctgaccaag cccaggcaac tctggcgcga cataatagcc attctcgggt tgataatcgt
    19381 aagtacaaag ttcccgaata catgctttca tcgcattggt atgatgttca tgaataataa
    19441 agttaggtat tgcagtttcc atatgcagcg ccgctactgt tgaaacaggc ccgccacaaa
    19501 catgtacctg aacggttgtg tcatatatat tggcataatc acaaattttc tttccctctg
    19561 taatgccacc acaaagacac aaatcaggtt gagctaccgc aattgattgt ttttccagta
    19621 aatcacgata tccccagcga gtataagaac gctcacctgt tgctatcgga atcgatgttg
    19681 aacgggcgac tttctgcata ttatccgaat ttaatggatg aattggctct tcatagagaa
    19741 aaatacgata tttctctatt gctttggcaa actggatcgc tgaatttgtt cctggaagag
    19801 aatgaatctc tacaataata tcagcatcgt cgcccattgc ttcacgcata gcggcaattc
    19861 ttgcctcgcc catttttaat tgttcggcca gtaataagcc agagtaattt ctatttttgt
    19921 tctgaaagac acaatcgccg ccatgcagat cgatttcaag cggatcaact tttatcgcat
    19981 tataaccttc tgcaatagcg gcacgcgctg cctctgcata ctcttcaggc gttactaata
    20041 tttgcctttt ttcgccccaa ccaaattgta actgactggc gtaagttctc aatttatcgt
    20101 tagtctttcc ccccagtagt tgataaaccg gcacgtttaa atatttacct ttaatatccc
    20161 ataaggcaat atcaatagcg ctcatacctg cgtaaaaaac attaccaccg cccattcccc
    20221 aaaatgtttt cctgaagagg aattcccaaa tcttctcaat gttcaacgga tcttcaccaa
    20281 tgatgagagg ggctaaatct ctgataatac ccacaccaga ttttgcacct gcaccatagg
    20341 ctaatcctac ttcgccaaca ccacttattc cagtatcggt attaacccga ataagtactg
    20401 gattataaga ggccatagtt tgatctcttt ttttgagttc gcagtcaaaa atttctacag
    20461 atgtaatctt cataaatagc ctcatttatt tagtatgtga aataatattt tctttctggg
    20521 cgttatcttt tttgtcatca attttcattg ataacaaaat agctacggcg ataaagcaaa
    20581 gaatcgcctc aacaataaat atccccgtgg caccataatg tgtagaaaca tagccaagtg
    20641 atttattgat cagaaaacca cccaaacttg aacaagagtt aataaacgca atactgattg
    20701 ccaggcctga tggtgaaagt aaagctgagg gtaaagccca gtacggaccg taataactga
    20761 gaatacctac accgtataat gttaaaccca tcatgcgcag cgtcatcgag ggcacaagtg
    20821 cgatcattaa aaaaccgcaa cctgcaacca acatcggtag tgcggcatgc catttccttt
    20881 cattcttttt atcggaatgc catccccata atggcatact gaacatcgcg aaaataaacg
    20941 gaatactcat aatcaaaccg acatcggtat ctgtgaaatc cgatgaaaat cctttaacca
    21001 accccggtaa ccagtaattt gccgccaacg ccgcagcctg aataaacata taagcaaatg
    21061 agagttggag taatactgga tttttaatga ttgacagaaa agatgcgttt gtatttttat
    21121 ttaacgctgc ggctttttcg ttatcagaat taagtttatc gaccaatgcg gtgacttgcg
    21181 atggtgtaag ccagcgggct ttttccggac gatctggcag taaataaaag gtcagcgccc
    21241 ccaataacac cgtaggaatg ccttcaatag caaaaagcca gcgccatcct tcatagttac
    21301 cggtattcat atgttcaata atccaaccag acattggtgc cgccagcaca gatgcaaccg
    21361 cgattgataa cataaaaaag ctattcactt ttccacgttc acgcgccggg aaccaacatg
    21421 ccaggtagta aaccatgcca gggaaaaaac ctgcttcaaa tattcccaaa aagacccgag
    21481 cgagcaatat atgctgggtg tctttggcaa agaaaattaa cgttgttact gttccccaac
    21541 caacaataat ggcgctaatc cattttcttg cacctaactt ttgcaatcca atactgctcg
    21601 gaatttgaaa aattaaatag gacacaaaaa atattgaaga tatattagca aaatcctctg
    21661 gtgtaatacc aagggattca ttcatgcgta aa
//
LOCUS       DAZFAH010000066        20993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFAH010000066 DAZFAH010000000
VERSION     DAZFAH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.066"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHJWB_002307"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHJWB_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4313942.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            753..977
                     /locus_tag="ACHJWB_002308"
     CDS             753..977
                     /locus_tag="ACHJWB_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313943.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHJWB_002309"
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHJWB_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4313944.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHJWB_002310"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHJWB_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4313945.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHJWB_002311"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHJWB_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4313946.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHJWB_002312"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHJWB_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4313947.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHJWB_002313"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHJWB_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO4313948.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAKMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHJWB_002314"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHJWB_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4313949.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..8983
                     /gene="tssK"
                     /locus_tag="ACHJWB_002315"
     CDS             7652..8983
                     /gene="tssK"
                     /locus_tag="ACHJWB_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4313950.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIC
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8988..9749
                     /gene="icmH"
                     /locus_tag="ACHJWB_002316"
     CDS             8988..9749
                     /gene="icmH"
                     /locus_tag="ACHJWB_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4313951.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9758..12535
                     /gene="tssH"
                     /locus_tag="ACHJWB_002317"
                     /gene_synonym="clpV"
     CDS             9758..12535
                     /gene="tssH"
                     /locus_tag="ACHJWB_002317"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4313952.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTAEARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNLVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAINVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHAYDPETMQEVLYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSR
                     VTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            12532..13275
                     /gene="vasI"
                     /locus_tag="ACHJWB_002318"
     CDS             12532..13275
                     /gene="vasI"
                     /locus_tag="ACHJWB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4313953.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13280..14692
                     /gene="tssA"
                     /locus_tag="ACHJWB_002319"
     CDS             13280..14692
                     /gene="tssA"
                     /locus_tag="ACHJWB_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO4313954.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14801..18235
                     /gene="tssM"
                     /locus_tag="ACHJWB_002320"
     CDS             14801..18235
                     /gene="tssM"
                     /locus_tag="ACHJWB_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4313955.1"
                     /translation="MLILAWIFLLVWIWWKSPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVDQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLLRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNKQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18246..19695
                     /locus_tag="ACHJWB_002321"
                     /pseudo
     CDS             18246..19695
                     /locus_tag="ACHJWB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19622..20104
                     /locus_tag="ACHJWB_002322"
     CDS             19622..20104
                     /locus_tag="ACHJWB_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4313956.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20148..>20993
                     /locus_tag="ACHJWB_002323"
     CDS             20148..>20993
                     /locus_tag="ACHJWB_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313957.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQE"
BASE COUNT         5015 a         5675 c         5754 g         4549 t
ORIGIN      
        1 gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaaa
       61 agtccttttc ttatttattt cgaagcgtaa aatgcggggt gatgaaggag tggtgatttt
      121 attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgcct cgcgagcgta ttaatatatc gtatgtccca aaaacagatg gccagacggc
      301 tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac
      361 atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat
      421 ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg gaagcacaac
      481 agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt caccagatag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca ataaataacc
      721 accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat atctccggga
      781 cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt
      841 ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc
      961 agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca
     1081 ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg
     1141 ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat
     1441 tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg gcggtgaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc
     1801 tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg
     1921 tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgaggcct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgcg gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta
     3841 ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc
     3961 cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagatgga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga
     4201 atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc
     4261 gtcacaaatt cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc
     4321 atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca
     4381 ctggtcccta ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcacggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt
     4561 tacgggtatt cccgtccggg gactggcctc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgtgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaataatc tgtggcctgg tgtcccactg ctttgatttg
     5401 tcagaagtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca gctcagtatt
     5581 accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc ggacgggcgg cgcgctgtct gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg tcagcagagc
     6001 attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag
     6061 acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct aatacagttg
     6121 cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga
     6181 gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat
     6241 atgctcacgg ataccctgct atcaacggag ggtacgccac actatccggg aatgactcac
     6301 cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact ccaggcactt
     6361 cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc aaaaatggcg
     6541 caacgccacc ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccatgagc
     6601 aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtaatgttt
     6781 gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg
     6901 ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa
     7261 taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg
     7381 accagatagc cagtacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaact acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 tctcaacaat gaactgctgg cacagggtaa gatcatgatt gacagcgcgt caggcacact
     7861 gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaacatttt ccggacgaga gaagccgcaa tatctgcctc gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ttgcattcag aagaaggtga cacgcgaaca ctgacgctgg gacaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg cattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca
     8401 tcacgcccgt cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgac ctgaccaaaa catttaaaac gctgttaccg gaagtcaaac gggcgctgca
     8581 taccgtactg tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca
     8641 ggccgatatc catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc
     8701 aaacatgccg gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatccccggc attccattgc gcgcactgat
     8821 ggtggctccc cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag
     8881 cggacaggcc tggacagaaa tggctgccgc cggggccgtt gcactgcatg tatccggcag
     8941 tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt
     9001 cactgtctct gccaccattt accggatacg acgaaaaaag ccagcgcaac taccatctcg
     9061 cgcttcgtgg caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg
     9121 taatgcgcct ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg
     9181 tgacggatgt tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga
     9241 ttatctcgtt tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct
     9301 ggtcaaacaa gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct
     9361 ggggtggaga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc
     9421 aggatctgct ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag
     9481 tggcagcaca ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc
     9541 ataaactgcg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg
     9601 ggcgttatca gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg
     9661 tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc
     9721 tgcaccagct aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac
     9781 gctggtaaaa cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg
     9841 tatgagtcag caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcgccat
     9901 gccacgcagt gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg
     9961 ccaggcgctg acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt
    10021 ttccccgatg ctggttgagt ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca
    10081 gcacagcgaa ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta
    10141 tataccgcct gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga
    10201 ctttgtgcag tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg
    10261 cgcaggcaca ctgacggacg ctgctgacac cctgcttgcc cgctacgcca aaaacatgac
    10321 cgcagaggcc cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct
    10381 gatgatcgac attctctgcc gccgccgcaa gaacaacctg gtggtggtgg gcgaagcagg
    10441 cgtgggtaaa agcgccttaa tcgaagggct ggcgctgcgc atcgtggcag gccaggtgcc
    10501 ggacaagctg aaaaacaccg atatcatgac ccttgacctg ggcgcattgc aggccggagc
    10561 gtcggtgaag ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc
    10621 cccggtgccg gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca
    10681 gcagggcggg ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa
    10741 aaccatcgcc gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct
    10801 gtcgcgccgc ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat
    10861 tctgcgcggt ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc
    10921 cttgcaggcc gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa
    10981 agcgattaat gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc
    11041 gaagcaaatc tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct
    11101 tgagcgcgag cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt
    11161 gcagtatgat gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca
    11221 gacgctggtc cgggagatta ttgcgctgcg ccagcagtta ctgggcgtgg cagaggacga
    11281 tgcggcgccg ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga
    11341 gtcagagtca gaacaggata ataccggtgc cgtaccggct gatgaggccg acagagaaca
    11401 accggaagag accgctgaaa cagtttcccc ggtacagcgg ctggcccagc tcactgccga
    11461 actggacgcc ctgcataacg accggttgct ggtctccccg cacgtcgata aaaaacagat
    11521 tgcggcggtg attgccgaat ggaccggcgt gccgctcaac cgcctgtcgc agaatgaaat
    11581 gtcggtcatc accgacctga cgaaatggct gggtgacacc atcaaaggcc aggacctggc
    11641 gattgccagc ctgcataagc atctactgac cgcacgcgcc gacctgcgtc gtccgggacg
    11701 cccgctcggt gcgttcctgc tggctggccc cagcggtgtg ggtaaaaccg aaaccgtcct
    11761 gcaactggca gagctgctct acggcggtcg ccagtacctg accaccatca atatgtccga
    11821 attccaggag aaacacaccg tctcgcggct gattggttcg cctccgggct acgttggcta
    11881 cggtgaaggc ggcgtactaa ccgaagcgat tcgccagaaa ccctactcgg tagtactgct
    11941 cgatgaagtg gaaaaagcgc acccggatgt gctcaacctg ttctaccagg cgttcgataa
    12001 gggcgaaatg gcagacggtg aaggccgcct gattgactgc aaaaatatcg tcttcttcct
    12061 gacgtccaac ctcggctacc aggtaatagt cgagcatgcc tatgacccgg aaaccatgca
    12121 ggaagtactg tatccggtgc tggccgactt cttcaaacct gccctgctgg cgcgtatgga
    12181 agtggtgccg tacctgccgc tgtcgaaaga gacgctcgcc accatcattg ccgggaaact
    12241 ggcccgcctg gataacgtgc tgcgcagccg ctttggcgcg gaggtgatta tagaaccgga
    12301 agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa aacggcgcga ggatgctgga
    12361 atcggtcatc gacggcaata tgctgccgcc gctctccctg ctgctgttgc agaaaatggc
    12421 ggcgaatacg gcgattgccc ggattcggtt gtcggcagtg gacggcgcat ttacggcaga
    12481 cgtggaagat gctcagaacg acgagtccgt cacaaaggat gaaacggttt tatgagcagg
    12541 ttgcgcgttt tccggcagac aggatggttt atcgcgggcc tgatgacggg cttacccgcc
    12601 actgccgcgc ccgcagaagc atcgtcaatg gccggtgtgg cggtcgccgt cgccaccacg
    12661 accccgccgg atgcgacagc cacgttacag gccatgcagt cctgtcggcg ggagtccgcg
    12721 gcgctggagc ggcttgactg ctatgaccgc ctgctggcac cgctgtcccc gtcagggttt
    12781 gacggggcgc tggtcaaagc cggttttgtg ggcgaggcct ggacccgcgc cacagagcag
    12841 gaaaaacgcc gcgagggcaa caccacagaa ctgctggtga cgcaggtgcc gggggagcgc
    12901 ccgacggtgg tgataaccac cccggctatc ggtcatgttc cgccacgccc ggtgctgatg
    12961 ttcagttgcg tggacaatat cacccggatg caggtggcgc tgatgcaccc tctggatgtc
    13021 catgatatcg ccgtcactct gaatgccgac aaccgggcat tgcgcagcca ctggtttgtg
    13081 cgcgagaacg gcaccctgct ggagtccagc cgggggttat ccgggattga tgaaattaag
    13141 caactgttcg gcgcaaaaac gctgacggtg gataccggcg cagacaatgc cgccggaaag
    13201 ctgaccttta acattgacgg gctggcaagg gcgattgctc ccctgcgcga tgcctgccac
    13261 tgggcgggag aataaaaata tggcaatgga cttacgcgat ccgaatgtct ggatatcgca
    13321 cctgctggaa aacctgccgg aagagaaact ggcatcggcg ctgaaagatg acaacccgaa
    13381 ctgggagtat atcgacggcg aaatcgtcaa gctgggatcg ctggcccaca gtcaactgga
    13441 tatcccggaa ctccagcgcc gggggctggt cattctggct tcggagagta aggattttcg
    13501 tctgctggcg catctgctga gaaccctgca acacgccggt gatcctcatc tcgcattgcg
    13561 cctgctggcg ctgtatgtgg agcattactg ggcggtggct gcgccgcaga atatggcgca
    13621 taaaaaacgc tttgccagcc aggtcattaa acgctttgaa acaggtattg aagttttttc
    13681 acagaacgcc gccacagcgc agcgcgatgc gctgtcaggg gaactggcaa aactggcgca
    13741 atgctggcag tcacataacg ccccggaact ggcacaggct actgatgatc tttttgccct
    13801 gtaccagcgt gcgtttaatc gtgcggcacc ggctcctgtc cccactccgg cggcctccgg
    13861 tagttcacca cagaccaccg ccacgtctga aagcggcgtg acgcaaccca gtgctccggc
    13921 tccccaaatc gtcatcgaca gtcacgacga caaagcctgg cgcgacacgc tgttaaaagt
    13981 ggcggccatt ttatgtgaac gccagccgga ttcaccgcag ggctaccgtc tgcgccgcca
    14041 tgcgctttgg cagaacatca ccagtacgcc ccaggcggaa agcgatggcc gtactccact
    14101 ggctgcggtc tccgccgata tggtggcaga ttaccatgct cagctcggta gtgccgatat
    14161 ggcgctgtgg cagcaggtgg agaaaagcgt actgctggct ccttactggc tggatggtca
    14221 ctgcctttct gcacagacgg cgctgcgtct gggttacaaa caggtggcag acgccatccg
    14281 cgatgaggtc atccgcttcc ttgagcgtct gccccagctt accgggctgc tgtttaatga
    14341 ccacacaccg tttatcagtg agcagacgaa acaatggctg gcagcttcgc ccgacgccaa
    14401 agttgcaccg gttgcgcaaa tcggtgagga atcgaaggca gccagagcct gttttgctga
    14461 gcagggtctg gaggcggcgc tgcgatatct ggacatgcta cccgaaggcg atccccgcga
    14521 tcagtttcac cgccagtacc ttgccgcaca gttgacggaa gaggcggggc tggtacagct
    14581 tgcgcagcag cagtaccgga tgttgttcag gatgggattg cagatgatgg tggcagactg
    14641 ggagccgtcc ctgcttgagc agctagaaca aaaattcacg gcagaacaat aaaagagaac
    14701 aggagttttt gtgttcagat taccaacacc ccgactactc agcggactca aatcagccct
    14761 gcgaccggcg atgccccggt ttaaagtctc tgctttctgg ctgctgatac tggcgtggat
    14821 ttttctgctg gtgtggatct ggtggaaaag cccgatgtgg acgctgtatg aagaacagtg
    14881 gctcaaacca ctggcgaacc gctggctggc aacggcggcg tgggggatta ttgccctggt
    14941 gtggcttacc gtccgggtga tgaagcgcct gcaacagctt gaaaaaatgc aaaagcaaca
    15001 gcgcgaggaa gccgttgatc cgctcagcgt ggaactgaac gcccagcagc gttatcttga
    15061 ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt ggcagttgcc
    15121 gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgc gcgaggggtt
    15181 tccgtccgac attatttatg ccccggaggg cgcacgcggc gcagaacaac gcctgtacct
    15241 cacgccccat gtcggtaaac aggcggtgat ctttgatatc gacggcacac tctgcgctcc
    15301 cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct ggctgaaaga
    15361 aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac ccgatctgct
    15421 taccgcagac aaacgccgcc gcgagcatct gttacagacg ctgcgcagcc gcttacagga
    15481 tatacgccag catcttcact gccagttacc ggtttacgtg gtactgaccc ggcttgattt
    15541 attacagggt tttgccgccc tgttccagtc actcaacaga caggatcgcg atgcgattct
    15601 gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac tggcgaacag agttgaatgc
    15661 tttctggcag acatgggtgg atcgaatgaa tctggcgttg ccggatctga tggtcgatca
    15721 gactcacacc cgcgcgtctt tattcagttt ttcccgccag atgcagggaa gccgtgaacc
    15781 gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat atgaacgtga tgctgcgtgg
    15841 tgtctatctc acctcttcgc ttcagcgtgg acagatggat gatatattca cccagtctgc
    15901 cgcccgccag taccggctgg gcaataaccc actggcgtcc tggcccctgg tggacaccgc
    15961 gccttatttc acccgcagcc tgttcccgca ggcattactc gcagagccta atctggcaac
    16021 agagagccgc gcctggctga tacgttcccg tcgccgcctg acggttttct ctgccacagg
    16081 cggcgtggca gcactgctgc tcatcaccgg ctggcatcac tattacaacg gtaactatca
    16141 gtccggcatc accgtgctta agcaggccaa agcctttatg gacgtgccgc ctccgcaggg
    16201 ggaagatgac tttggcaacc tgcaactgcc gctcctgaac ccggtacgcg atgccacact
    16261 ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt accagggacg
    16321 acgtatcggg ccttatgtgg aacagaccta tctgcaactg ctggagcaac gttacctgcc
    16381 ctcgctgttt aacgggctgg tcaaagcgat gaacgccgcg ccgccggaga gtgaagaaaa
    16441 actcgcggtg ctgcgcgtaa tgcgaatgct ggaggacaaa agcggacgta acaatgaggt
    16501 ggtgaagcag tatatggcaa aacgctggag cgaaaaattc cacggccagc gcgatatcca
    16561 ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc atgcagagcg
    16621 tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc ccgtggtatc
    16681 agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga gcctgaaaac
    16741 gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag ggccgacctt
    16801 tgaccaggtg tttacatctg ccgatgacaa caaactggtt gttccacagt ttcttacccg
    16861 ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac tgacggcgat
    16921 ggattcctgg gtacttaacc ttacccgcag cgtgaaatac agtgacgccg accgcgcgga
    16981 aatccagcgc cagttgaccg agcagtatat cagcgactac accgccacct ggcgggccgg
    17041 gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg gggcgctgga
    17101 gcaggttatc agcggcgacc agcctttgct gcgggcgctg accgtgcttc gtgacaacac
    17161 acagccaggc gtcttttctg aaaaactctc tgccaaagaa cgggatgagg ccctggcaga
    17221 accagactac cagttgctca cccgtctcgg gcatgaattc gccccggaaa acagtaccct
    17281 ggcagtacag aaagacaaag aaagcacgat gcaggccgtg tatcagcaac tcaccgagtt
    17341 gcaccgctat ctgctggcaa tccagaacgc gcctgtacca gggaaatcgg cgctgaaagc
    17401 cgtgcagtta aggcttgatc agaacagcag cgatccgata ttcgccaccc gccagatggc
    17461 aaaaacactg cccgctccgc tcaaccgctg ggtcggcagg ctggctgacc aggcctggca
    17521 tgtggtgatg gtggaggccg ttcactatat ggaagtggac tggcgcgaca gcgtggtgaa
    17581 accgtttaac aagcaactgg caaataacta tccgtttaat ccgcgttctg cacaggatgc
    17641 ctcactggat gccttcgaac gcttctttaa accggatggc atactggata ccttctacca
    17701 gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg ataacaatgt
    17761 cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc gtgacatctt
    17821 cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat cgctttccgg
    17881 caataaacgc cgcagtgtac tgaaccttga cggtcagtta gtcgattaca gccagggccg
    17941 taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg aaagtaagct
    18001 gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg ggccgtgggc
    18061 gcagttccgc ctgttcgggg ccggacaact gaccggagta caggatggca actttaccgt
    18121 gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca cggaagataa
    18181 cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt actgaggagg
    18241 cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac ccgcgcgggc
    18301 tgccggaatt cagtgccatc cgcgaggaaa taaacaaagc cagccacccg tcacagcctg
    18361 agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc aacggtgtgg
    18421 atttacacac cgccacctac tatacgcttg cccggacacg gacacaggga ctggcgggat
    18481 tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat aagttctggc
    18541 cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga accggcaata
    18601 ttctgcgtca gcaaatctcc tttgcggaat ccgacctacc actgatctac cgcacagagc
    18661 gggcattgca gcttatctgc gataagctcc agcaggtgga actgaagcgc gttccgcgcg
    18721 tggagaatct gctctatttt atgcagaaca cgcgtaaacg gcttgacccc cagctgaaga
    18781 gtaacactga gaacgccgca cagaccacgg tcagaacgct gatttatgtc ccggaaacac
    18841 aggcatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctacct gagatgaaag
    18901 tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag caaggcagta
    18961 cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca gcattgtggt
    19021 ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc gctcagggcg
    19081 cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg ctgcaacaac
    19141 ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg cgtgttgccg
    19201 acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg aatgaggcac
    19261 tcaaaacccg cgcacagagt agcccgcagt tgcgtggctg gttgcagact cgccaggact
    19321 tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactaacc ctttcctata
    19381 tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaacaccc gttgagtctc
    19441 tgttgacgca gtaccatgat gcccgtgcgc agaagcagaa tacagatgcg ctggaaaaac
    19501 aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaag aataacgtca
    19561 tgccagaggc ggcaaccggt accacggccg aaaaataatt atttaattaa aggagattat
    19621 catggcaaat ataagttatt tatctttgag tggagagaca caagggctaa tttcagcagg
    19681 ttgctcaacg cttgattctg tcggaaacaa agcgcagcca gaacataaag atcagatcat
    19741 ggtttatgcg ttaatgcatt ccatctcaag aagtcaaaat gtcaatcatc atgaattaat
    19801 tatcaccaaa ccggttgata aatcctcacc attgctggca aaagcactta gtgacaatga
    19861 aaaaatggca atatgtgaat ttatactcta ccgaaccagt aaagcgggta tttaccagcc
    19921 ttattataaa ataaatctgt caaaagcccg aatatcaagt attgattttg tcacaccgca
    19981 tgctgtcctg gaaaaagaac tggaaccaca ggagagaatt gcctttattt atgaagatat
    20041 ttcctgggag catacgttag ccggtacgaa tgcaatgagt aaatggcagg atcgtgtaca
    20101 gtaacttact gtaataacat aataaattac tattgcggag agataaaatg gctggtttaa
    20161 ggaataacaa caacacacag aatgcacagt gggctgatta tgtgggtgac attcttcggg
    20221 gggctcagcc gataaaccag ctagtacctc agcaccctta tcttaacgac gtcccactca
    20281 ttgatgagtt aaggcatcag aacactcatc atgttattct cttgacactt gatgttgcaa
    20341 aaaaaatact cagcccaata acatcatttg attacattca ttttatcact acacatcctt
    20401 caggtataaa agatacactc gcctggttag ttaacgcgag taaattaatg actgagtttg
    20461 atgacaatgg gaaaattata tttaacctta atgccttaaa gtatacaaaa gcaagttact
    20521 ttgaaatact tggagagaaa tatataaaaa tcacaacatc cagcccctgg cttttagaaa
    20581 agctcggtaa atatattttc tcttcacgag cgcctcaagt tctggaactc gctattggct
    20641 ggcgaggagc tttatctgaa tcaataaaag gagttaagtt ctgtatctgg ttttcagtag
    20701 cctggagaac catagaattt attatgagtt cagagcgaga tctggttaat tttcttggcg
    20761 atttttcaat ggatgtggcc aaagcagtta tagccggtgg tgttgcaacc gctattggaa
    20821 gcctggcttc ttttgcctgt gttagctttg gctttccagt aattcttgtc ggaggagcaa
    20881 ttttactgac agggatagtg tgtacagttg ttttaaatga aatcgatgct caatgccatt
    20941 tatcagaaaa attaaaatat gcaattagag atggactaaa acggcaacag gaa
//
LOCUS       DAZFAH010000067        20799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFAH010000067 DAZFAH010000000
VERSION     DAZFAH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.067"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            315..1979
                     /gene="asnB"
                     /locus_tag="ACHJWB_002324"
     CDS             315..1979
                     /gene="asnB"
                     /locus_tag="ACHJWB_002324"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4313958.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2360..2436
                     /locus_tag="ACHJWB_002325"
     tRNA            2360..2436
                     /locus_tag="ACHJWB_002325"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Met,seq:cat)
     gene            2445..2529
                     /locus_tag="ACHJWB_002326"
     tRNA            2445..2529
                     /locus_tag="ACHJWB_002326"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2479..2481,aa:Leu,seq:tag)
     gene            2553..2627
                     /locus_tag="ACHJWB_002327"
     tRNA            2553..2627
                     /locus_tag="ACHJWB_002327"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2585..2587,aa:Gln,seq:ttg)
     gene            2662..2736
                     /locus_tag="ACHJWB_002328"
     tRNA            2662..2736
                     /locus_tag="ACHJWB_002328"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2694..2696,aa:Gln,seq:ttg)
     gene            2752..2828
                     /locus_tag="ACHJWB_002329"
     tRNA            2752..2828
                     /locus_tag="ACHJWB_002329"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2786..2788,aa:Met,seq:cat)
     gene            2877..2951
                     /locus_tag="ACHJWB_002330"
     tRNA            2877..2951
                     /locus_tag="ACHJWB_002330"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2909..2911,aa:Gln,seq:ctg)
     gene            2989..3063
                     /locus_tag="ACHJWB_002331"
     tRNA            2989..3063
                     /locus_tag="ACHJWB_002331"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3021..3023,aa:Gln,seq:ctg)
     gene            complement(3217..4392)
                     /gene="ubiF"
                     /locus_tag="ACHJWB_002332"
     CDS             complement(3217..4392)
                     /gene="ubiF"
                     /locus_tag="ACHJWB_002332"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4313959.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGNSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARNYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4538..5962
                     /gene="miaB"
                     /locus_tag="ACHJWB_002333"
     CDS             4538..5962
                     /gene="miaB"
                     /locus_tag="ACHJWB_002333"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4313960.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6115..7155
                     /locus_tag="ACHJWB_002334"
     CDS             6115..7155
                     /locus_tag="ACHJWB_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4313961.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7152..7619
                     /gene="ybeY"
                     /locus_tag="ACHJWB_002335"
     CDS             7152..7619
                     /gene="ybeY"
                     /locus_tag="ACHJWB_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4313962.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7709..8587
                     /gene="corC"
                     /locus_tag="ACHJWB_002336"
     CDS             7709..8587
                     /gene="corC"
                     /locus_tag="ACHJWB_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4313963.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8612..10150
                     /gene="lnt"
                     /locus_tag="ACHJWB_002337"
     CDS             8612..10150
                     /gene="lnt"
                     /locus_tag="ACHJWB_002337"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4313964.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10228..12105)
                     /locus_tag="ACHJWB_002338"
     CDS             complement(10228..12105)
                     /locus_tag="ACHJWB_002338"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO4313965.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12560..13468
                     /gene="gltI"
                     /locus_tag="ACHJWB_002339"
     CDS             12560..13468
                     /gene="gltI"
                     /locus_tag="ACHJWB_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4313966.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAVPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13638..14378
                     /gene="gltJ"
                     /locus_tag="ACHJWB_002340"
     CDS             13638..14378
                     /gene="gltJ"
                     /locus_tag="ACHJWB_002340"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4313967.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14378..15052
                     /gene="gltK"
                     /locus_tag="ACHJWB_002341"
     CDS             14378..15052
                     /gene="gltK"
                     /locus_tag="ACHJWB_002341"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4313968.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            15052..15777
                     /gene="gltL"
                     /locus_tag="ACHJWB_002342"
     CDS             15052..15777
                     /gene="gltL"
                     /locus_tag="ACHJWB_002342"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4313969.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15895..16830
                     /gene="rihA"
                     /locus_tag="ACHJWB_002343"
     CDS             15895..16830
                     /gene="rihA"
                     /locus_tag="ACHJWB_002343"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4313970.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16914..18584
                     /gene="hscC"
                     /locus_tag="ACHJWB_002344"
                     /pseudo
     CDS             16914..18584
                     /gene="hscC"
                     /locus_tag="ACHJWB_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(18644..20095)
                     /gene="djlC"
                     /locus_tag="ACHJWB_002345"
     CDS             complement(18644..20095)
                     /gene="djlC"
                     /locus_tag="ACHJWB_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO4313971.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(20092..20799)
                     /locus_tag="ACHJWB_002346"
     CDS             complement(20092..20799)
                     /locus_tag="ACHJWB_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4313972.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         5138 a         5377 c         5215 g         5069 t
ORIGIN      
        1 agctcctgca atatattgaa ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc
       61 atccggcatg aacaaatcgc actttatcag caatttggcc cccgtaaaat ataaccattc
      121 aataaaaccc gctaaaaatt atcgattcat caataaatac atcctcacac cagacccttt
      181 ttcaccaatc gacaatcacc attacgtttt ttattttttc accgccaaat cgcttgcgca
      241 cagtgctgct ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccaggt
      301 taacggagaa ggttatgtgt tcaattttcg gcgtattcga tatcaaaaca gacgcagttg
      361 agctgcgtaa gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt
      421 ccggtattta tgccagcgat aacgccattc tcgctcacga acgtctgtca attgttgacg
      481 ttaacgcggg ggcgcaacct ctctacaacc aacaaaaaac ccacgtactg gcggtaaacg
      541 gtgaaatcta caaccaccag gcattgcgcg ctgaatatgg cgatcgttat cagttccaga
      601 cagggtctga ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg
      661 acgacttgca gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcctacctga
      721 ttggtcgcga ccatctgggg atcatcccac tgtatatggg ctatgacgaa cacggtcagc
      781 tgtatgtggc ctcagaaatg aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc
      841 cggcggggag ctatttgtgg agccaggacg gcgaaatccg ttcttattat catcgcgact
      901 ggttcgactt tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggctc
      961 tggaagattc cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctgctttctg
     1021 gtggtctgga ttcctcaatt atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg
     1081 aagatcagga acgctctgaa gcctggtggc cgcagttaca ctcctttgct gtaggtctgc
     1141 cgggttcacc ggatcttaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc
     1201 acgaaattca cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca
     1261 tcgaaactta tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta
     1321 agatcaaggc gatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg
     1381 gcggttatct ttacttccat aaagcgccca acgctaaaga actgcatgaa gagacggtgc
     1441 gtaaactgct ggccctgcat atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct
     1501 ggggcgtgga agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca
     1561 ttaacccgca ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat
     1621 gttttgagtc atacctgccc gcaagcgtgg cctggcggca gaaagagcag ttctccgatg
     1681 gcgtcggtta cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctaatc
     1741 agcaactgga aactgcccgc ttccgcttcc cgtacaacac gccaacctca aaagaagcgt
     1801 atctgtaccg ggagatcttt gaagaactgt tcccgcttcc gagcgccgct gagtgcgtgc
     1861 ctggcggtcc ttccgtcgcg tgttcttccg ctaaagcgat cgaatgggat gaagcgttca
     1921 agaaaatgga cgatccatct ggtcgtgcgg ttggtgttca ccagtcggca tataagtaag
     1981 aaatgcgaaa cagccccggt gaaatccggg gctgtttcag ttattgaggt gcttgttgat
     2041 tctgatatgc agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac
     2101 tgtaggccag tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc
     2161 ctgtacgaca aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac
     2221 gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt
     2281 gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag
     2341 gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc actcataatg
     2401 atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat
     2461 tggtagacgc accagattta ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct
     2521 cccgcaccat tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg taaggcaccg
     2581 gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatct tcttcgagta
     2641 agcggttcac cgcccggtta ttggggtatc gccaagcggt aaggcaccgg tttttgatac
     2701 cggcattccc tggttcgaat ccaggtaccc cagccatcga agaaacaatc tggctacgta
     2761 gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt
     2821 agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat ttaaaaacca atttgttggg
     2881 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg
     2941 taccccagcc aatttattca agacgcttac atcgtaagtg cacccagttg gggtatcgcc
     3001 aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag
     3061 ccacattaaa aaagctcgct tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga
     3121 atgcgatgtt gacacgtctt atccttcaat gtcggatgcg acgctgccgc gtcttatccg
     3181 acctacggtt ggcacgcatc cggcaatgtt gtaaggctac aaccctaacg catatttcag
     3241 cgcctgacgt ttcaacacgc cagcacgctc tgccgccatt aacccgagat tacgcacaaa
     3301 acgcagcggc ggcagattat tactgaatcc ggcataaaac agatccatac cactttgcat
     3361 aatgaagtta tccgccatgc gccgcatctg gtaacgcttg aggacaggat aactggccca
     3421 cgcttcaccg tagttgcggg cattcaccag aacatcaatc agggcatcga catcacgata
     3481 accaagattc accccctgcc ccgccagcgg atggatggta tgcgcggcat cgcccaccag
     3541 cgcaagcccc ggctgcacat actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc
     3601 ggcaagcggt gtaacgtaac ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag
     3661 ttgcgccata ttcatattct gcaactggcg aatacgcgcc ggagagtcat accacaccag
     3721 cgatgcccag ttatcaaaca acggcagaaa cgcacgcggt ccgtccgggg taaattgctg
     3781 ccaggtgctg ttgccgggat cgttctcaca ctgtacgcta atcaacatgc acgactgcgc
     3841 atactgccag gcatgaacgc caattcccgc catctgccgc acctgcgaat ttgcgccgtc
     3901 ggcaccaatc accagtttcg cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag
     3961 atcattatct cgatgtaatg caatcagcga gcctggcaca cgtaacgtta ctttcggatg
     4021 cgcttccagc gcctgccaca gcgcctgttg caggacggtg ttttccacca tatagccaag
     4081 caacggtagc ttaagttcag cggcgtcaaa caccacatgc gccgtttccc actcccacgt
     4141 ttccagtctg cggtaaggat ggcaacgcat agcctgtact gcatcccaga cccctaaccc
     4201 tttaagcaat gataccgatg cggcgctgat cgccgagatc cgcacgtccg gttggctatc
     4261 agcgacaaac ggtgcaggtt ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc
     4321 cagccccagc gccagcgcgc cgccgaccat tcctccgccg acaatggcaa tttccgttgg
     4381 ttgatttgtc atggtcaatc atcctgttag caatatgctt taagtttaca ggatttttac
     4441 ccccaagggc tgataacgct catactggtc acaacggcag caaagcatta cactatgcgc
     4501 cctgcattcc tggctactat ttcgcaagag caagtcgatg accaaaaaac tccatattaa
     4561 aacctggggc tgtcagatga acgagtacga ttcatcgaag atggccgatc tgctggatgc
     4621 cacccacggc tatcaactga ccgacgtggc ggaagaagcg gatgtgctgc tgctgaacac
     4681 ctgctcaatc cgcgagaagg ctcaggaaaa agtcttccat cagttggggc gctggaaact
     4741 gttaaaagag aagaatccag acctgattat cggcgtcggt ggctgcgtgg catcgcaaga
     4801 aggcgagcac attcgccagc gcgcccacta tgtcgatatt atttttgggc cgcaaacgct
     4861 gcaccgtctg ccggagatga tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat
     4921 cagcttcccg gaaatcgaga agtttgaccg tctgccggaa ccgcgcgccg aagggccgac
     4981 cgcgtttgtc tccatcatgg aaggctgcaa taaatattgc acctactgcg tggtgcctta
     5041 cacccgtggt gaagaggtaa gccgtccgtc cgacgatatt ctgtttgaga tcgcccagct
     5101 tgcggctcag ggcgtgcgtg aagtcaacct gctcggtcag aatgtgaatg cctggcgtgg
     5161 tgagaactac gacggcacca ccggatcgtt tgccgatctg ttgcgtctgg ttgctgcgat
     5221 cgacgggatc gatcgtattc gctttaccac cagccatccg atcgaattca ccgacgatat
     5281 catcgaagtg tatcgcgaca cgccggagct ggtgagcttc ctgcatctgc cggtacagag
     5341 cggttccgat cgcattctga acctgatggg acgtacccat acggcgctgg agtacaaagc
     5401 gatcatccgt aaactgcgtg cggcgcgtcc agatattcag atcagttctg atttcatcgt
     5461 tggcttccct ggcgaaacca ccgaagactt cgagaaaacg atgaagctga ttgccgacgt
     5521 caatttcgac atgagctaca gctttatctt ctctgcacgt ccgggtacac cagccgccga
     5581 tatggttgat gatgttccgg aagaagagaa gaagcaacgt ctgtatattc tgcaagaacg
     5641 tatcaatcag caagcgatgg catggagccg ccggatgctc ggcaccaccc agcgtattct
     5701 ggtagaaggg acttcgcgta agagcatcat ggagctttcc gggcgtaccg aaaataaccg
     5761 cgtggtcaac ttcgagggca caccggatat gatcggtaaa ttcgtcgatg tagaaattac
     5821 cgacgtctac ccgaactctc tgcgcggtaa agtggtgcgt actgaagatg aaatgggtct
     5881 gcgcgtggca gaaacaccgg aatcggtgat tgcccgtacc cgcaaagaaa acgaccttgg
     5941 cgtgggttat tatcagccgt aattctcagg cccgccgttc cggtgggcct tgttttttct
     6001 ctctttatcc ccatctttat cgcaatgctt gcgccttttt cctgtggcgt gaataatttc
     6061 cttataggcc ggtgaatgtt cagcttccgg cgacatacag ataagaggaa cggtttgaac
     6121 atagacactc gcgaaatcac cctggagcca gcagacaacg cgcgtctgtt gagcctgtgc
     6181 ggcccgtttg atgacaacat caagcagctc gaacgccgtc tcggcatcga gatcaatcgc
     6241 cgcgacaacc actttaaact gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt
     6301 ctgcgtagcc tgtatgtcga tacagccccg atgcgcggtc agattcagga tatcgaaccg
     6361 gaacagatcc accttgcgat taaagaagcg cgagtactgg agcaaagtgc ggagagcgtg
     6421 ccggagtacg gcaaagcggt caatatcaaa accaaacgcg gcgtaattaa gccgcgcacg
     6481 ccaaaccagg cgcagtacat cgccaatatt ctcgaccatg acattacctt cggcgttggc
     6541 ccggcgggta cgggtaaaac ctacctggca gtggctgcgg cagttgatgc cctggagcgt
     6601 caggagattc gccgtattct gctgactcgt ccggcggtcg aagccggtga gaaactgggc
     6661 ttcctgcctg gcgatttaag ccagaaagta gacccgtatc tgcgcccgct gtacgacgcg
     6721 ctgtttgaaa tgctgggctt tgagaaagtt gagaaactga ttgagcgcaa cgttattgaa
     6781 gtcgcgccgc tggcctatat gcgtggtcgt acgctgaacg acgcgtttat cattctcgat
     6841 gagagccaga acaccaccat cgaacagatg aagatgttcc tgacccgtat cggttttaac
     6901 tcaaaagcgg ttatcaccgg cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca
     6961 ggcttacgtc acgccatcga agtgctggcc gatgtcgaag agatcagctt taacttcttc
     7021 cacagcgaag atgtggttcg tcaccctgtg gtggcgcgta tcgttaacgc ctatgaagcc
     7081 tgggaagaag ccgaacaaaa acgaaaagcg gcgctggcgg cagaacgcaa gcgcgaagaa
     7141 caggaacaaa aatgagtcag gtgatcctcg atttacaact ggcatgtgaa gataattccg
     7201 ggttaccgga agagagccag tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg
     7261 aagaatcgga agtgactatt cgcgtggtcg ataccgccga aagccacagt ctgaatctga
     7321 cctatcgcgg taaggataag ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg
     7381 gcatggaaat gtcgctactg ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag
     7441 ctcaggagca aggcaaacca ctggaggcgc actgggcgca tatggtggtg cacggcagtc
     7501 tgcatttgtt aggttacgat cacatcgaag atgacgaagc agaagaaatg gaagccctcg
     7561 aaacagagat tatgcttgct ctgggctatg aggatccgta cattgccgag aaagaataat
     7621 ttgccaggca tttgactggc agacgtcccc gccgttggtg attaacgacg gcgttttgat
     7681 taactaacat gagaacccat acgacgccat gagcgacgac aattcacaca gtagtgacac
     7741 gataagcaac aagaagggat ttttctccct gttactcagc caacttttcc acggtgaacc
     7801 gaaaaaccgt gacgaactgc tggcgctgat ccgtgattcc gggcagaacg accttatcga
     7861 cgaagatacg cgcgatatgc tcgaaggggt gatggacatc gcagaccaac gcgttcgcga
     7921 catcatgatc ccccgctccc agatgattac cctgaaacgc aaccagacgc tggacgaatg
     7981 ccttgatgtc atcatcgagt ccgcccactc acgtttcccg gtgattagcg aagacaaaga
     8041 tcacattgaa gggattctga tggcgaaaga cttgctgccg tttatgcgca gcgatgctga
     8101 agccttcagc atggacaaag tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt
     8161 agaccggatg ctgaaagagt ttcgctctca gcgttaccac atggcgatcg ttattgacga
     8221 attcggtggg gtttccggtc tggtgaccat tgaagacatc ctggaactaa ttgttggtga
     8281 gattgaagac gagtatgacg aagaagatga tatcgacttc cgtcagctga gtcgtcatac
     8341 ctggaccgtg cgcgcactgg cttccattga agacttcaac gaagcgttcg gcacccactt
     8401 tagcgatgaa gaggttgaca ctatcggtgg tctggtgatg caggcatttg ggcatcttcc
     8461 ggcgcgtggc gaaactatcg acatcgacgg ttaccagttc aaagtggcga tggccgacag
     8521 tcggcgtatt attcaggttc atgtcaaaat cccggatgac tcaccccagc cgaagctgga
     8581 tgaataaaac cgaaactgga tagataacta catggctttt gcctcattaa ttgaacgcca
     8641 gcgcattcgc ctgctgctgg cgttattatt cggtgcctgc ggaacgctgg ccttctctcc
     8701 ttacgacgtc tggcctgcgg cgattatttc gttgatgggg cttcaggcgt tgacctttaa
     8761 ccgccgtcca ctccagtctg ccgctattgg cttttgctgg ggatttggcc tctttggcag
     8821 cggtattaac tgggtctatg tcagcatcgc gacctttggc gggatgcctg gcccggttaa
     8881 catcttcctg gtggtgctgc tggcggcgta tttgtcgctg tataccggac tgtttgctgg
     8941 cgtgctgtcg cgtctgtggc cgaaaaccac ctggctgcgc gtggcgattg ccgccccttc
     9001 tctctggcaa gtgaccgagt ttctgcgcgg ttgggtactg acaggcttcc cgtggttaca
     9061 gttcggctat agccagattg atggcccgtt aaaagggctg gcaccgataa tgggcgtgga
     9121 agccattaac ttcctgctga tgatggttag tggcctgctg gcactggcgt tggtcaaacg
     9181 caactggcgt ccgctggtgg tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta
     9241 catccaatgg tttaccccac aaccggagaa aaccattcag gtttcgatgg ttcagggcga
     9301 tattccgcaa tcactgaaat gggatggaga tcagcttctt aatacgctga agatttacta
     9361 caacgcaacg gctccgctga tgggcaaatc atcgttgatt atctggccgg agtcggcgat
     9421 aaccgatctg gaaattaatc agcaaccgtt cctcaaagca ctggacggtg agttgcgcga
     9481 taaaggtagc tcgctggtaa ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta
     9541 cgatacctac aacaccatca tcacactggg taaaggtgca ccgtacagct acgaatcagc
     9601 cgatcgctat aacaaaaacc atctggtgcc gtttggcgag tttgtcccgc tggagtcgat
     9661 tctgcgtccg ttagcaccgt tctttgatct gccgatgtcg tcgttcagcc gtgggccata
     9721 tatccagccg ccgctgtcgg taaatggtat tgagcttact gcggctattt gctacgagat
     9781 cattctcggc gagcaagtgc gcgataactt ccgcccggat accgactatc tgctgactat
     9841 ctccaacgat gcgtggtttg gtaaatctat tggtccatgg caacacttcc agatggcgcg
     9901 aatgcgtgcg ctggagctgg cgcgcccact gttgcgcagc accaacaacg gcattacggc
     9961 ggtgattggc ccgcagggtg agattcaggc gatgatcccg cagttcaccc gcgaggtgtt
    10021 aaccactaat gtgacaccga ccaccggact cacaccgtat gcacgtaccg gcaactggcc
    10081 gctgtgggtg ctgacggcgc tgtttggttt tgccgctgtg ctgatgagtc tgcgagcacg
    10141 taaacgttga tgtgcctgat gtatcctgca tgagcaaaac gtactttctc attgagcttt
    10201 ggggcatggt tattatgccc ctttttctta cggttcaggg atacaagcca cattgccata
    10261 ttggggatga cgaataattg cctgcggatc aagcgagcgc gtcctgtcca tttggatcaa
    10321 cccgttcttc gccatgacaa atatttttct accatccgct gaaaaacgaa ggtgattaac
    10381 gtagctggca ggataaacgc caataatctg cccggcatta atattgagca ggatgacata
    10441 accttgcagg ccatatgaca ccaacgctaa atattgaccg tccgggctaa taccctcaac
    10501 ggaataaacg ctcctgctac accattgcgt cataatgctg gcttgttgat cccctacgct
    10561 tatacgtgaa atcccctgac tatcggcaaa aataacctcg ccgttttcag gtcgaaaacg
    10621 catcgccgat atcgttccac gcgcatcacc tgcgggaaag tcttctttct gatgcgtgat
    10681 ggtattgatc cgcacgatgt tacctctgcc tgctcccgga accagtaatg ttctttcatc
    10741 cggggaaagt gccataaccc aagcgcctgg tttaccgctc gtccccgtcc cgtcgtattt
    10801 ctccagcggg atatccgcca catgctgata agtcaccaga tcaatcgctg acacactatt
    10861 actggtgcga ttcatcaccc aggcttttgt gccatcacgc gagaggataa ggctgtcagg
    10921 atgtgcaccg gtggtaatcg actgaataat ctcccctgtc gccacgttaa tgaccgataa
    10981 cgcattcgct tcaaatgaag tcgcataaat caacttttca tccgggctta atgccagcga
    11041 tctgacgccc tgatctttac agccaccaca atgtttgact gacttttctt tcgctatggg
    11101 cgctgtaaat gtatgcagga ttttcttttg tccgaggtcg aaaacaacaa tgctgttcgt
    11161 gttttcaatt gcggcataca acatattacc atcccgactg atcgccatat catggatatc
    11221 ggcaatctca gcgagcaaat caccactggc ctgttcacga cttaccggag cggataatgt
    11281 ttccctgagg gcgttgcgtt cctccgctaa ctgctgcttt ttttgccgtc gctcttgttc
    11341 aatctcctgt ggataaaacg cttcacgcag gctttgcctg acttgtaaaa ctgactcatc
    11401 catctgctgc tgcgcaagcc agatagcccc ggtcagaaga agactgcccg caacaataat
    11461 cccgccttcc gtaacgagtc ggttttgccc agataaacgc gcgctcagcc aacccaacgc
    11521 gccacccgca atcaggccgc caatgtggca agcgttatct attcctgttt gccgggcacc
    11581 gtacaacagt gtcagcgcca ccatacccaa cagaggaaat actcgccgct ggtttttatg
    11641 gtatgtgccc gtacctgaag caaactgtgt tgctatcgct gcaccagcta ttcccagaat
    11701 cgccccggat gccccaacgc tgatgactat ctgctcactt tcgcgtaacg cataacaggc
    11761 gctgatgagc ccaccaaaga cgccggagaa taaccaggta atcaggagtc gccatttccc
    11821 cagaatctgt tccgccacgc ctcccacgac caggaatgca aacatattca tcagcagatg
    11881 agaaacacta ctgtgcagaa agatactgct caataagcgc cagggttcac ccgacaaagt
    11941 aagaggcgca acgtttgcac cgagatggat aagtgcggat tgatcgacct cttcataact
    12001 catccattgt gcgagataac aaaatatccc aatacagagt gcaaaaagga taagggttat
    12061 tgcgggtaat tgaacattta acggcttaac cgaagatgca gacataaggt gtttcttcct
    12121 tgaaaagaca ggtaaaaagc atttttcccg tgtgcagtta tcatcccggc aaacgtccca
    12181 tgccgaaaag ataattctgt ccgaaatatg actttttcag cctaaaatta caatcgtaat
    12241 taattcaccc ctaagtattt tttggcatgt aagttgcttt atttactgtg taagcgatga
    12301 aatcagccgc gacgcaacca ggttgcacca ccacaaatca gcggtagcac caaaaaggtg
    12361 cggtttaaga attttgcttc ggaatggtgc ggcgcatctc gcaaaaacaa acaataaaac
    12421 agcaatatct ttacatttag ccaaactaaa tgttacctaa caggcacaac actgcacaat
    12481 aaagttgcag acgataacaa cacaaacact cacaacgggt atccatgcgt tcttaacgca
    12541 gaagataaag gagttggata tgcaattacg taaacctgcc acagcaatcc tcgccctggc
    12601 gctttccgca ggactggcac aggcagatga cgccgtcccg gcagcaggca gtacgctgga
    12661 caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tacctttctc
    12721 ttattacgac aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt
    12781 tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga ttccgattac
    12841 ctcacaaaac cgtattccac tgctgcaaaa cggcactttc gattttgaat gtggttctac
    12901 caccaacaac gtcgaacgcc aaaaacaggc ggctttctct gatactattt tcgtggtcgg
    12961 tacgcgcctg ctgaccaaaa agggcggcga tatcaaagat tttgccgacc tgaaagacaa
    13021 agccgtagtc gtcacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga
    13081 gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgcaccct
    13141 ggaaagcggt cgtgccgttg cctttatgat ggatgatgcc ctgctggccg gtgaacgtgc
    13201 gaaagcgaag aaaccagaca actgggaaat cgtcggcaag ccgcagtctc aggaagccta
    13261 cggttgcatg ttgcgtaaag acgatccgca gttcaaaaag ctgatggatg acaccatcgc
    13321 tcaggtgcag acctccggtg aagcggaaaa atggtttgat aaatggttca aaaatccaat
    13381 tccgccgaaa aacctgaaca tgaatttcga actgtcagac gaaatgaaag cactgttcaa
    13441 agaaccgaat gacaaggcac tgaactaatt acaagaacca ggggcggaaa ttccagccct
    13501 ctcgattgtt acgtagcacg gacagactat acgcctgatg gtcgttcccc atcgggcctg
    13561 aaaaccgcaa tacgctgggt aacaatcttc gagggtagca gttaacgctg ctaccctctt
    13621 ttttctggag tagatttatg tctatagact ggaactgggg tattttttta caacaagccc
    13681 cgttcggcaa caccacctat cttggttgga tctggagtgg ttttcaggtg acgatcgctt
    13741 tgtcgatctg cgcctggatt atcgctttcc tcgtcggttc atttttcggc attttacgta
    13801 ccgttccaaa ccgttttctc tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg
    13861 tgccgttgat tgtgcaattc tttacctggt atctggtgat cccggagctg ttgccggaga
    13921 aaatcggcat gtggtttaag gccgagctgg atcccaatat tcagtttttc ctttcatcca
    13981 tgctctgcct ggggctgttt accgccgccc gtgtttgcga acaggtacgc gccgccattc
    14041 agtcgttacc gcgtgggcaa aaaaatgccg cgctggcgat gggcctgacg ctaccgcagg
    14101 cttatcgata cgtactgctg cctaatgctt atcgcgttat cgtcccgccg atgacttccg
    14161 agatgatgaa cctggtgaaa aactccgcca tcgcctcgac tattggtctg gtggatatgg
    14221 cggcgcaggc gggtaaattg cttgattact cagcccacgc ctgggagtcg tttaccgcta
    14281 ttacgctggc gtatgtgttg attaacgcgg ttatcatgct ggtaatgacg ctggttgaac
    14341 gtaaagttcg cctgcctggc aacatggggg gcaaataatg tacgagtttg actggagttc
    14401 cattgtccct tccctgccat atctgctcga cgggctggtg atcacgctga aaatcaccgt
    14461 cacggcagtc gtgattggta ttttgtgggg caccatgctg gcggtgatgc gcttgtccag
    14521 ctttgcgccc gtagcgtggt ttgccaaagc ctacgttaac gtattccgct cgattccttt
    14581 agttatggtt ttgctgtggt tttacctgat cgtgccaggt tttctgcaaa acgtgctggg
    14641 attatcacca aaaaatgata tccgtttaat ttcggcgatg gtggcgtttt cgatgtttga
    14701 agcggcctac tattcagaga ttatccgtgc tggtattcaa agtatttctc gtggtcagtc
    14761 gagcgccgcg ctggcgttgg gaatgactca ctggcagtcg atgaaactga ttattctgcc
    14821 gcaggcgttc cgcgcgatgg tgccgcttct gctcactcag ggcatcgtac tgttccagga
    14881 tacctcactg gtgtatgtgt tatctctggc cgatttcttc cgtaccgcct caaccattgg
    14941 tgagcgtgat ggtacgcagg ttgagatgat cctgtttgcc ggatttgttt atttcgttat
    15001 tagtcttagc gcgtcgttgt tggtcagcta cttgaaaaga aggacagcat aatgattacc
    15061 ctgaaaaatg tttcaaaatg gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa
    15121 gtgaaaaaag gcgaagtggt ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg
    15181 attaaaaccg tcaacggcct cgaaccagtg cagcaaggtg aaatcaccgt cgatggtatc
    15241 gtggttaatg acaagaaaac cgatctggca aagctgcgtt cccgcgtcgg gatggtattc
    15301 cagcatttcg agctgttccc tcatctgtcg attatcgaaa acctgaccct ggcgcaggtg
    15361 aaagtgctta aacgcgataa agcgcccgct cgtgaaaaag cattgaaatt gctggagcgc
    15421 gtgggacttt ccgcacatgc caataaattt ccggcgcagc tttccggcgg tcagcagcag
    15481 cgtgtggcaa tcgctcgcgc gttgtgtatg gatcctattg cgatgctgtt cgacgaaccg
    15541 acatcggcgc tggatccgga gatgatcaac gaagtgctgg acgtgatggt ggaactggca
    15601 aacgaaggaa tgaccatgat ggtggtgacc cacgaaatgg gttttgcccg taaagtggcg
    15661 aatcgggtga tctttatgga cgagggtaaa attgtcgaag actcgccgaa agacgctttc
    15721 ttcgatgatc cgaaatcgga ccgcgcgaaa gacttcctcg cgaaaatcct gcattaatca
    15781 ctctggcgcg catacccaag tgcgcgccat ctcacgcttt atcgtttcgc ccctcgtatc
    15841 tgttacatcc tggcgttacc cttgtcgcaa agaagcacac aacaaggagc aacaatggca
    15901 ctgccaattc tgttagattg cgacccaggt catgacgacg ctatcgcaat agttctcgcc
    15961 ctcgcctcac cagagcttga tgtcaaagca attacgtctt ccgccggaaa ccagacacca
    16021 gaaaaaacct tacgcaatgt tcttcgtatg ctgaccttgc ttaatcgcac cgatattccg
    16081 gtagcaggcg gcgcggtaaa accgttaatg cgtgagttga ttatcgcgga caatgtgcac
    16141 ggcgaaagcg gtctggatgg cccggcatta ccggaaccga cgttcgcacc gcaaaactgt
    16201 acggcggtag agctgatggc gaaaacgctg cgcgaaagtg cggaacctgt cacgattgtg
    16261 tctaccggac cgcaaactaa cgttgccttg ctgctcaata gccatccgga actgcatagc
    16321 aaaattgccc gtatcgtgat tatgggcggc gcaatggggc ttggtaactg gacgcctgcg
    16381 gctgaattta atatttacgt tgacccggaa gcggcagaaa ttgtcttcca gtcagggatc
    16441 ccagtggtga tggccggtct ggatgttact cacaaagcac aaatccacgt tgaagacacc
    16501 gagcgtttcc gcgcgattgg caaccctgtt tcaaccattg ttgccgaact gctggatttc
    16561 ttcctcgaat atcataaaga cgaaaaatgg ggctttgtcg gtgcaccgct gcatgaccca
    16621 tgcaccatcg cctggctgtt gaaacccgag ttgtttacca ctgttgagcg ctgggttggc
    16681 gtggaaacac aggggaaata tagccagggt atgacggttg ttgattatta ttatctgact
    16741 ggcaataaac cgaatgccac tgtaatggtc gatgttgatc gtcagggctt tgttgattta
    16801 ctggctgatc gtctgaaatt ttacgcttaa cacacgtcat tgattaagat caccacgcgt
    16861 tacctgggtg gtcttaatct gtttatctct ttttctttca agatataaat gcaatggata
    16921 atgcagaact cgccattggt atcgatctcg gcactaccaa tagtttaatt gccgtctgga
    16981 aagacggtgc cgcgcaatta attccaaata agttcggtga atatttaaca ccatccataa
    17041 ttagcatgga tgaaaataat catattttag tcggaaaacc ggctgtatca cggcgtactt
    17101 cgcatccgga taaaacggca gcgttattta aacgtgcaat gggcagtaat accaactggc
    17161 ggttaggcag cgacacattt aacgcgccag aactgtcctc tttggtatta cgctcattaa
    17221 aagaagatgc cgaagaattt ctgcaacgtc cgattaaaga tgtggtgatc tccgttccgg
    17281 cttatttcag tgatgaacaa cgcaagcata cccgtttagc agcggagtta gccgggttaa
    17341 atgcggtacg cttaattaat gaacccacag cagctgcgat ggcgtatggc ctgcataccc
    17401 aacaaaatac ccgttcgctg gtgtttgatc tcggtggcgg cacgtttgac gttacggtgc
    17461 ttgagtacgc cacgccggtg attgaagttc acgcctccgc tggcgacaac tttcttggtg
    17521 gcgaagattt tacccatatg ctggtcgatg aggttttaaa acgcgcggat gtcgccagga
    17581 ccacgcttaa cgagagtgaa ctgggagcct tgtacgcctg tgtggaagcg gcaaaatgta
    17641 gcaatcaatc gccattgcac attcgctggc agtactagga tgaaacgcgg gagtgcgaat
    17701 tttacgagaa cgaactggaa gatttgtggt tgtcgctgct caatcgcttg cgagtgccga
    17761 ttgaacaggc gttgcgcgat gcacatctga agccgagtca aatcgacagt ctggtgctgg
    17821 ttggcggcgc gtcacaaatg ccgctggtgc agcgaatcgc cgtgcgtctg tttggcaaat
    17881 taccgtatca aagttacgac ccgagcacca ttgtcgcgct gggcgcagca atccaggccg
    17941 cctgccgctt acgcagtgaa gatattgaag aggtgatcct cactgatatt tgcccttact
    18001 cgttgggcgt tgaagttaac cgccagggcg tttccggcat tttctcgcca attattgaac
    18061 gaaacaccac agtacccgtg tcgcgtgtag aaacttactc gaccatgcac ccggaacagg
    18121 attcaattac ggttaacgtc tatcagggag aaaaccacaa agttaaaaat aacattctgg
    18181 ttgaatcctt cgatgtgccg ttgaagaaaa ccggggctta tcagtcgatt gatattcgct
    18241 ttagttatga tatcaacggc ttgcttgaag ttgacgtgct tctggaagat ggcagcgtta
    18301 agtccagagt cattaatcat agcccggtaa cattgagcgc gcagcagatt gaagagagtc
    18361 ggacgcggtt atccgcattg aaaatttatc cgcgcgatat gctcatcaac cgcaccttta
    18421 aggccaaact ggaagagttg tgggcgcggg cgctgggtga cgagcgagaa gagattggcc
    18481 gggtgatcac cgattttgat gcggcgttgc agtcaaacga tatggcccgc gtcgatgagg
    18541 tcaggcggcg ggcgagcgtt tatttagcca ttgagacctc ataatcacgg ctgccggatg
    18601 cggcgtaaat gctgcatccg gcatcggcgc aaaaaacaca tcgtcaacta aacaacatcg
    18661 cgattttgcc gagagcataa cctataacca gcaccgttaa actgattttg ataatgctgg
    18721 taatgctctg tggtttttca ccctcatcat ttttttcttc atggttttca ggtgttggct
    18781 catcgacatc ttcatcctcc ggctcgagtt ttactttcgt caacagcatc cgggtgataa
    18841 tggcctctaa tggatgagca tctccgcctt ccagccttgt attaagacgg gccagcatct
    18901 cttgtttcgg aaacgcttca agtactacct gttccgctac ccgatacaaa tccagtaacg
    18961 gtgaatcttc ttctgtctcg ctaccgactc tcgatttcca ccacgcccat gctttgcgtt
    19021 tgaagaataa cgactcaata aacgattgac ctaacggcga taattttccg ctatgtaaaa
    19081 tttccgccca taacagccgc gtccgggcgc tctcaccagg aacatatttc agcggttgac
    19141 cttgcgcatc taccatgctc cgcgcttcga taaccatcac taataacgcg aaataatccg
    19201 ggcaatgacg cttgctccac tgcaacaaca aattatcagc ttgtgtagat gggtaacttt
    19261 cccggtacgc gcagagatcg gcaagcaggc tttccccctc gccgcagtac aacctttgag
    19321 cacacaagta atatcgggca tcttcgcttt ctggttcttc catttgccac tgtttggcta
    19381 caggaatgag ttccgccata cctgtttgca ccgagctgta ccagcgcaac agtttgcgat
    19441 gtaactttgc atcatcagga atgaaccacg gaccttccat cgccaaaaac gcagtgacgt
    19501 agtcaggatg gtaacggcag caacgttcca gcgcgaagta atactcaaca gtctggttct
    19561 gcaacgcaac gggatattcc agaaggctaa ggaaatcgaa acaatcacca gcctgaatgg
    19621 cctcaagaaa agcctccctt tcttcctcat ccattccttc gctgtcatta aacgaatgcc
    19681 agttcaaacg ctctgccagc aaagaagcac aatttaatga aagatatcgc gcttctatag
    19741 cgattgcaca cagcggccag cgtaattgat cgacatcctc catgttccag gtatttaatt
    19801 gctgaataaa tttctgccag gcggagggct gaaaacgatc actttctgaa tccagtaatg
    19861 tcctgaatgc acgcagtatt tcatgttcag cggcggcatc tttttcttca tcatctgctt
    19921 cctcgacagg atttaccgcc agtcgcagcg cctcttcata ggcctggcgt aatgctttaa
    19981 acccctgcgg atcggtttcg ggatgacata acggtaagcg agcaagataa gcctggcgga
    20041 taatgtctat ttgcgtcgtg ctttcaattt cgaggatttg ccaacatgtt ttcataggtc
    20101 gctcacatcc ttaagcgtta ccgggcattc aggctggaca gttaatggct gccagggaag
    20161 cgtggcatag aaacgatcgc ttcccgctgc cagctcttcg aacatccgac tcccggcgct
    20221 atcgtatttg tacagggtta gcgcctcgcg tagcgaagtg ttgtcttcgc actgagaaga
    20281 ttgccagtag agacgtccta acgaataggc ggaaaaatag tgcatccagc tgtgatagta
    20341 atggtgagct cgtacataga cgcgcgattg cagccataaa ctctcttctt ccgtcagcca
    20401 gttgttgagc acaccgaccc gtgacagaaa ccccattcga acataatccc aggctttgat
    20461 tcccccttct ccacagcaca tagcggtttg ggcaacgaat tgggtataaa tcaaccctct
    20521 ctccgagcca ccagcaatta atgctttcca ctcttcaggg gaagagcgat gccatgtcag
    20581 atataagcca gctaaatcat tggcatgacc atcatcagtc atctgataaa tagtctgaat
    20641 taaatcatca cgattattta ttccccagga tgatgagaga tcaaattctt ttataccggt
    20701 atacattttt ggcgagtcat gcgcgggttt gcaaccctga ttaagtattt ccatgggcgc
    20761 agaaagcgca aaaagaagat attgttcttc cttattcat
//
LOCUS       DAZFAH010000068        20697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFAH010000068 DAZFAH010000000
VERSION     DAZFAH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.068"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..96)
                     /locus_tag="ACHJWB_002347"
     CDS             complement(<1..96)
                     /locus_tag="ACHJWB_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4313973.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRG"
     gene            complement(238..453)
                     /locus_tag="ACHJWB_002348"
     CDS             complement(238..453)
                     /locus_tag="ACHJWB_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4313974.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVECLNKLGAKVSLDDVFPPEHKAA"
     gene            572..1225
                     /locus_tag="ACHJWB_002349"
     CDS             572..1225
                     /locus_tag="ACHJWB_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4313975.1"
                     /translation="MKWYELARSRMKELGITQEKLAEELGMTQGGIGHWLRGSRHPSL
                     DEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFSHVQAGMFSPELRTF
                     TKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGMLILVDPEQAVEPGDF
                     CIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVGKVIASQWPEETFS"
     gene            1425..1593
                     /locus_tag="ACHJWB_002350"
                     /pseudo
     CDS             1425..1593
                     /locus_tag="ACHJWB_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1571..1843
                     /locus_tag="ACHJWB_002351"
     CDS             1571..1843
                     /locus_tag="ACHJWB_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4313976.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            1846..2238
                     /locus_tag="ACHJWB_002352"
     CDS             1846..2238
                     /locus_tag="ACHJWB_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO4313977.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLSDAKQGIQAKLE"
     gene            2302..2460
                     /locus_tag="ACHJWB_002353"
     CDS             2302..2460
                     /locus_tag="ACHJWB_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313978.1"
                     /translation="MNKKYIVEVIERETKEVIKHFEFDNYRKADRVEEGLLRQSNLEK
                     FDVVMRCE"
     gene            2593..2904
                     /locus_tag="ACHJWB_002354"
     CDS             2593..2904
                     /locus_tag="ACHJWB_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO4313979.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMDALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEMGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            2980..3114
                     /locus_tag="ACHJWB_002355"
     CDS             2980..3114
                     /locus_tag="ACHJWB_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313980.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            3099..3251
                     /gene="kil"
                     /locus_tag="ACHJWB_002356"
     CDS             3099..3251
                     /gene="kil"
                     /locus_tag="ACHJWB_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4313981.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            3508..4113
                     /locus_tag="ACHJWB_002357"
     CDS             3508..4113
                     /locus_tag="ACHJWB_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO4313982.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAVKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            4113..4496
                     /locus_tag="ACHJWB_002358"
     CDS             4113..4496
                     /locus_tag="ACHJWB_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313983.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNNQQQQSSNHQNQPEYDDSIPF"
     gene            4520..4813
                     /locus_tag="ACHJWB_002359"
     CDS             4520..4813
                     /locus_tag="ACHJWB_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO4313984.1"
                     /translation="MPAPLYGADDVRRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            4824..4988
                     /locus_tag="ACHJWB_002360"
     CDS             4824..4988
                     /locus_tag="ACHJWB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO4313985.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            4985..5491
                     /locus_tag="ACHJWB_002361"
     CDS             4985..5491
                     /locus_tag="ACHJWB_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313986.1"
                     /translation="MKQMSLIEMDGFLKGKCFPRDLKVNETNAEYLVRKFGELESKIE
                     TSLRECRSAGLTIDNIEAKCAALAAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIIND
                     ALHHSETPATDAFLAEVRAQAFNDLCSVFVKDATVAGLDDGDIVTVKEATDALLHCAE
                     QLRKGGNQ"
     gene            5488..6366
                     /locus_tag="ACHJWB_002362"
     CDS             5488..6366
                     /locus_tag="ACHJWB_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4313987.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDDLLSEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKSRDQENEDIALTVGKLRVELEGKDSKI
                     ANLTAERDALREGEMGDARHSNTRAAADIYFQLVEECEIPAGGSLVEYVDDMREKLEA
                     AENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAREIKPAKGEVLVVVSGFTGC
                     GKSAIAGEIEIAMKAIGVPVQWTNGDAEKRMTGADWLTAIEMYKPTVRIVEVNVPRAA
                     GIRIKGDAGEQNDEKP"
     gene            6719..6844
                     /locus_tag="ACHJWB_002363"
                     /pseudo
     CDS             6719..6844
                     /locus_tag="ACHJWB_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            7223..7393
                     /locus_tag="ACHJWB_002364"
     CDS             7223..7393
                     /locus_tag="ACHJWB_002364"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313988.1"
                     /translation="MPPQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGNALFWM
                     PLPEPPQEVNRG"
     gene            7386..7670
                     /locus_tag="ACHJWB_002365"
     CDS             7386..7670
                     /locus_tag="ACHJWB_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4313989.1"
                     /translation="MANLQLAVKGEFFDAMIRGEKTEEYRLCNGYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            7743..8087
                     /locus_tag="ACHJWB_002366"
     CDS             7743..8087
                     /locus_tag="ACHJWB_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4313990.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            8161..8406
                     /locus_tag="ACHJWB_002367"
     CDS             8161..8406
                     /locus_tag="ACHJWB_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4313991.1"
                     /translation="MSEVNLIVPNDWVTEQKLIEITGLRPGTIERARKQSWLVGREYL
                     HVAPDGIPKENSECMYNRKAIDQWVESMSKKQPGARR"
     gene            8452..9756
                     /locus_tag="ACHJWB_002368"
     CDS             8452..9756
                     /locus_tag="ACHJWB_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4313992.1"
                     /translation="MAKTAYPTGVENHGGSLRIWFMYKGTRVRESLGVPDTPKNRKIA
                     GELRASVCFSIKTGNFDYAAQFPDSPNLRKFGAESKEITVADLAEKWLDLKKLEISTN
                     AMGRYSSIVRNMVPRIGGNRLASAVTQEELLFIRKDLLTGYHVLKRGQKTPIKGRSVP
                     TVNNYMNTMAGMFQFAADSGYIKENPFAAISQLKRSRTEPDPLTRDEFVRLINAFKHQ
                     QLKNMWSLAVYTGVRHGELVSLAWEDIDLKAGTMTIRRNHTLTKEFTLPKTEAGTNRV
                     INLIQPAIDVLKNQAEMTRLGKQYQVEVKLREYGRTEMHPCTFVFNPQIVTRNGLAGH
                     HYAVGSINQSWETAMRRAGIRYRKAYQSRHTYACWSLTAGANPNFIAKQMGHSDAQMV
                     YRVYGSWMAENNQDQVSILNQKLSEFAPSMPHAVGSDVIKQA"
     gene            complement(9774..10088)
                     /gene="fliE"
                     /locus_tag="ACHJWB_002369"
     CDS             complement(9774..10088)
                     /gene="fliE"
                     /locus_tag="ACHJWB_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4313993.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLQQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            10303..11961
                     /gene="fliF"
                     /locus_tag="ACHJWB_002370"
     CDS             10303..11961
                     /gene="fliF"
                     /locus_tag="ACHJWB_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4313994.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            11954..12949
                     /gene="fliG"
                     /locus_tag="ACHJWB_002371"
     CDS             11954..12949
                     /gene="fliG"
                     /locus_tag="ACHJWB_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4313995.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLVNRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            12942..13628
                     /gene="fliH"
                     /locus_tag="ACHJWB_002372"
     CDS             12942..13628
                     /gene="fliH"
                     /locus_tag="ACHJWB_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4313996.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            13628..15001
                     /gene="fliI"
                     /locus_tag="ACHJWB_002373"
     CDS             13628..15001
                     /gene="fliI"
                     /locus_tag="ACHJWB_002373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4313997.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            15020..15463
                     /gene="fliJ"
                     /locus_tag="ACHJWB_002374"
     CDS             15020..15463
                     /gene="fliJ"
                     /locus_tag="ACHJWB_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4313998.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            15460..16587
                     /gene="fliK"
                     /locus_tag="ACHJWB_002375"
     CDS             15460..16587
                     /gene="fliK"
                     /locus_tag="ACHJWB_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4313999.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            16692..17156
                     /gene="fliL"
                     /locus_tag="ACHJWB_002376"
     CDS             16692..17156
                     /gene="fliL"
                     /locus_tag="ACHJWB_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4314000.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            17161..18165
                     /gene="fliM"
                     /locus_tag="ACHJWB_002377"
     CDS             17161..18165
                     /gene="fliM"
                     /locus_tag="ACHJWB_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4314001.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASASGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            18162..18575
                     /gene="fliN"
                     /locus_tag="ACHJWB_002378"
     CDS             18162..18575
                     /gene="fliN"
                     /locus_tag="ACHJWB_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4314002.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            18578..18943
                     /gene="fliO"
                     /locus_tag="ACHJWB_002379"
     CDS             18578..18943
                     /gene="fliO"
                     /locus_tag="ACHJWB_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4314003.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALITIIALILAAAWLIKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            18943..19680
                     /gene="fliP"
                     /locus_tag="ACHJWB_002380"
     CDS             18943..19680
                     /gene="fliP"
                     /locus_tag="ACHJWB_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4314004.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            19690..19959
                     /gene="fliQ"
                     /locus_tag="ACHJWB_002381"
     CDS             19690..19959
                     /gene="fliQ"
                     /locus_tag="ACHJWB_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4314005.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            19968..>20697
                     /gene="fliR"
                     /locus_tag="ACHJWB_002382"
     CDS             19968..>20697
                     /gene="fliR"
                     /locus_tag="ACHJWB_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4314006.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHL"
BASE COUNT         5507 a         5036 c         5440 g         4714 t
ORIGIN      
        1 accgcgctgg gtaagcgttg acaggttgat gagtaaatca gtttcagcgc gatcaacgtc
       61 acgctgtgat agtttgctgt aacttgttct ttccatttct taagatttcc aatagtgaat
      121 agttagttga aaggtatgcg tggaaacgca tatggcctta gttggtcaga tatattggga
      181 ctcgcttttc agcgacgtag gacgaatgtc cgttgttaca aagagcggat ccgcttatta
      241 agcagctttg tgttccggcg ggaacacgtc atcaagactg acttttgcgc ctaacttgtt
      301 taggcactca acaagagcac ggcatgtttt aaggtctggg aagcgacgac cagattccca
      361 atgtccgata gctccctgtg tgcatccaac tgccttagca agtgttgttt gagagatatt
      421 cagtgactct cgatattttc gtaggttgct catatgccct ccatagtaat cacaatgcaa
      481 taatacgata tgtactttta gaatgcaaac aaaaaaatac atcttgtgca tggatgattt
      541 tagtacagag cgtaataata agggtatgaa aatgaaatgg tatgaactgg ctagatccag
      601 aatgaaagag ctcggcataa ctcaagagaa gttagctgaa gagcttggta tgacgcaggg
      661 tggaattggt cactggttgc gcggatctcg tcatccatct cttgacgaga ttggtgtggt
      721 gtttaaatac cttggtattg ataacgtctc attcaaccac gacggtacat tttcacctgt
      781 tggcgaatac tcatctgccc ccgttaaaaa acaatatgag taccctgttt tttctcatgt
      841 tcaggccggg atgttctcgc ctgagcttag aacctttacc aaaggtgatg cggagagatg
      901 ggtcagcaca accaaaaaag ccagtgattg tgcgttttgg cttgaagttg aaggtaattc
      961 catgaccgcg ccaacaggat ccaagccaag ctttcctgac gggatgttaa ttctcgttga
     1021 ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttggtg gtgacgagtt
     1081 taccttcaag aaactgatca gggatagcgg tcaggtgttc ctacaaccac taaacccgca
     1141 atatccaatg attccatgca atgatagctg ttccgtagta gggaaagtta tcgccagcca
     1201 gtggcctgaa gagacattta gttaacagcc tcaccactct aaaacacaca acaataaccc
     1261 gaccttagcg tcgggttttc tttttccaaa atataaatca ataaaacaca aagtgttata
     1321 aaaaactaaa ccatatttag aacattttgt attgactcag taaagtacac atcgtactat
     1381 ttagccatca gcaggaagct ggaagccaaa cggaacagac tggcatgctc tttaaacaac
     1441 gtcgaacact cgactacgtg gctgaaaagc cagatcaccc aaccacataa gctgtgggat
     1501 gcaatgccga agcaaccgtc tcaggagggg cttcgagatt gcatcgccaa agtttattcg
     1561 ggaggaatct atgtccagaa aaacagaatt taaaggcacc gcagcttctc gccgtagagc
     1621 tcgtcgtgca aatctgcaaa gtcaggaggc gatcagctcc gacaagctac acaggccaac
     1681 cccttcacga gtggtcttgc aatgcaagcg caaaccagca atgagagcag aagtaataac
     1741 actgacaacg ttgaccagaa aatatgaagg ctcaacttgt cttccaaatg tagccattta
     1801 cgcggcaggc taccggaaat caaaacaact gacagcgagg taattatggg tcaggaagaa
     1861 aaatatgagc ttaaaaagct cattggagaa gacgccatag aagaaattgc agcattaaca
     1921 acagctataa agaatattag gtatgcgcta aatacgctta tctcctcatg cgacaaaaat
     1981 agcagggaat ttttgatact tggcgcagct ctaggaatag ttgatgcggc aacgcttcac
     2041 ctaattactc atgacgatat tcttattgag ccgtatgaaa cattactgct tgtcaggcaa
     2101 aaaatggctg atgccgcagc aaatggagac cttcaacttt acatcgactt aaggaaagta
     2161 ttaaggcgaa tggtcaaaac tgaaggagat atccccctga gcgatgctaa acaaggcata
     2221 caagcaaagc tggaatgacc attatcctga tgaagtatcg aaagcacagt cagcaattag
     2281 caaagctctt ggggaagaat gatgaataag aaatacattg ttgaagttat agagcgagaa
     2341 acaaaagaag taattaaaca tttcgaattt gataattata gaaaagctga ccgcgtagaa
     2401 gaaggattgt tgcgacaaag taatctcgaa aaatttgatg ttgtcatgcg atgcgaataa
     2461 gcgcctatag cagatttgcg agtctgctat gtgagcaatg tcgctcgtaa ctaaacagga
     2521 gccgacttgt tctgattatt ggaaatcttc tttgccctcc aatgtgaggg cgattttttt
     2581 gacggaggaa atatgaaatt acgtgtctgg catatcccgc aagtacctat gaaaccgttc
     2641 attgcagaag tggcaagtgt tgaagagggt gttcgcctga tggatgcact ggctgattat
     2701 gacgcctttc agtatgacaa caacatcaag cctgattact gcaatgctaa cggccttgag
     2761 atgtgggatg agagccttac cgatgaagat ttatcagaga tggggcttac tgatcgctgg
     2821 gtggattggt acagcgaatg ccaatgttac gacgacccac gtaaatatct cgaaagcctg
     2881 aaagaagaaa catcagccgc ctaagcgcgg ctttaccgca taccaataac gcttcactcg
     2941 aggcgttttc gttatgcaat caaatataag gagttaccca tgatgcactt tcagctcgcg
     3001 ggtagcggcg tcatgtccgc tttctacccg cacgaatctg aattatcacg ccgagttaaa
     3061 caattaatca gagcagcaaa gaaacaactg gaggcgttat gcgcaatgaa atagccatca
     3121 atcaccagat gcttcgtgct gcacagaaca aagcagtaat agccagattt attggtgatt
     3181 ccaaaatgtg gcttgaagca aataaagcga tgaaatcagc tatcaacctt ccgtggtatc
     3241 gcaggaaatg agttttacag ataactggtc agacgaagaa ttcattcgtc agatgaaaga
     3301 tttaatcggt aacgaaggag atattcatgt cacttgcaac cacagtgaag gagagcaagt
     3361 tacagagacg catgtacacg cagaaagctc tctggtatcg ccataatggt gaccgcgaag
     3421 gaatgcgggt atgccttaat ttgtccagag tcgaagtatt aaaccagcgt tatttccttg
     3481 ggccatgtcc attctgagaa caaacatatg agcaaagaat tttacgcaag actggcagct
     3541 attcaggaga atctgaacgc gccaaaaaat cagtacaact cattcggtaa atataaatac
     3601 agaagctgcg aagatattct tgaaggcgtt aagccgctac tgaatggtct gtttttatca
     3661 atcagcgatg aagttgtgtt gattggtgat cggtattacg tgaaagccac ggcaactatt
     3721 accgatggcg aaaacagtca tacggcaacc gctcttgcac gagaggaaga aagcaagaaa
     3781 ggaatggatt ctgcacaagt tacgggagct acaagctctt atgcacgcaa gtattgcctc
     3841 aatggtttgt tcggcattga tgatgcgaaa gatgcagata ccgacgagca taaacatcag
     3901 cagaacgcag cagtaaagca gtcaaaacca tcacctacac ctgaacaggt tctaaaagca
     3961 ttcactgacg cagcaatgca gaaaaacacc gtagaagagc ttaaacaggc gttcgccaaa
     4021 gcatggaaga tgctcgaagg cacaccggag cagcacaaag cgcaggacgt ttacaacatc
     4081 agacgagacg aattagaagg agcggctgct taatggcaca ttcgattact gtaagactaa
     4141 acaagcccgc aagagagttt caggccgggg aaaatatcgg attcaacatc cgtgctggcg
     4201 ttcagtatta cgatcgccag acaaaaaaga aagaatggac aaactacagc gccgttgtat
     4261 ttgccaagcc gggagcgcaa gcggattact accgtagcgt tcttgttgaa ggtggcattg
     4321 ttgaaattac cggagaaaac atcagggttg atgtttatca ggggcaaaat ggtcaatcaa
     4381 tcactcttga attactgaat gcaaagattg gatttgcaac ttcaggaaat aaccaacagc
     4441 aacaaagtag caatcatcaa aatcaacctg aatacgatga ttcaattccc ttctaagtta
     4501 gcaaaataag gattccatta tgccagcgcc tttgtatggt gcggatgacg tgcgccgctg
     4561 ttccggcaat tccgtatcgg aggtgctgga taaattcaga aaaaactacg acctgataat
     4621 gtcgctaccg caggaaacga aagaggaaaa ggaatttcgc cattgcatat ggcttgcaga
     4681 gaaagaagaa cgcgagcgaa tttaccagac atcaatccga ccattccgca aagccacata
     4741 tacccacttc cctgaaattg acccgcgcct gcgtaattac cgctcacgct atggcgctat
     4801 cagtaatgac tgaggaattt accatgagag gacttgcata caatcccggc attcttccgg
     4861 cagaaatgat tattcgccaa cgcgtaaagc caatgccatc gagagaggaa ttgcttaaga
     4921 gaaattcttt tccatcagta aatcaaaaca aatatctgaa tgcgatgtgg cggagtggga
     4981 agaaatgaaa caaatgtcac taattgagat ggatggtttt ctgaaaggta aatgcttccc
     5041 acgagattta aaggttaacg aaacaaacgc tgaatatctg gtgcgtaagt tcggtgaact
     5101 tgaatcaaaa attgaaacgt cgttgcgtga gtgtcgttct gccgggctca cgattgacaa
     5161 cattgaagcc aagtgcgctg cgctggccgc tgagaatgcg gggctgaaac acgcaatggc
     5221 cgtaactctt gagcatgtgt cggtcacgga tgcagggcag gctggtgttg ctgcaatgat
     5281 tatcaacgat gccctacacc atagcgaaac cccagccacc gacgctttcc tggctgaagt
     5341 gcgggcgcag gcgtttaatg acctttgctc ggtgttcgtt aaggacgcga cggttgccgg
     5401 gctggacgat ggcgacatcg ttacggtgaa agaagcgacg gacgccctgc tgcattgtgc
     5461 ggaacagctt cgcaaaggag gcaaccagtg agcgaaatta attaccaggc actgcgtgag
     5521 gcggcggaac gtgcaattcc agcaatggaa cgcctgttaa tgttgccagc tgatgatgat
     5581 ttgttaagtg aacaggaact taaagattac ggtgtggata ttgatgcgct caacgccttc
     5641 aaatttctga ccggaccaga aaccgtgctg gcgctgctgg atgaacggga aaggaaccag
     5701 caatacatca aatcccgcga ccaggagaac gaggatattg cgctaacggt agggaagctg
     5761 cgcgttgaac tggaaggcaa agacagcaag atagccaatc ttaccgccga acgcgatgct
     5821 cttcgtgaag gtgagatggg cgacgctagg catagcaaca cacgggccgc agctgatatc
     5881 tacttccaac tggtcgagga gtgcgaaatt cctgctggcg gttctctggt cgagtacgtt
     5941 gacgatatgc gcgagaagct tgaagccgca gagaataacc tcattgatag tgaatgccat
     6001 gttgctgaac tggaagaagc gctacgcgat aagcaggcgt tacttgaagc atcagaaaag
     6061 cgcatagcag aactggaagc acgggaaata aaaccagcca aaggtgaagt tcttgtcgtt
     6121 gtttctggtt ttactggttg cggaaaaagc gccatcgccg gggaaataga aatcgcgatg
     6181 aaggctattg gtgtaccggt tcagtggact aatggcgatg cggaaaagcg catgacagga
     6241 gctgactggc tgacagcgat tgagatgtac aaaccaacag tgcgcatcgt ggaagttaat
     6301 gtgccgcgcg ccgcaggcat tcgcatcaaa ggagacgcgg gagaacaaaa cgatgaaaaa
     6361 ccgtaaagca aaattacttc ttttatcgcg ttcgacgggg tacgagaagc taccaatcag
     6421 caatcacaaa cgggcggtga tcggcatgta tggtagggtg gtattcgcat ttaactataa
     6481 acccaccgcg tcgcggaaca gacgagagaa ggggtacgca gtgcgatgaa aaatcgtaag
     6541 gcaaagatgc tgatagcaaa gcccgggcag cgtatccgta tctcaaatcg gcttgttatt
     6601 tactatagat atctgggttt tagggggtgt tcctctattc gtttttacgg tgtctggcga
     6661 aacagaagcg cagcacaaaa ccgctggaaa aaccactttc gcactaaagg agagtgatat
     6721 gaccactttc accgacaaag aactgattaa agaaatcaaa gagcgtatca gcagcctgga
     6781 cgttcgagac aatattgaac gccgggctta tgaaattgca ctggcatcgc tggaagcaga
     6841 tccagttgct tatattttca aacatcctgc cgggaaatta ttctgggctt taacggatga
     6901 aagcaataaa gagcaagcgg acgttattcc tgtttatgct gccgcgcctg cgtcggttgt
     6961 gccggataat gcatcagagc ctcttgctta tgcttacaaa gagcttacgc ctgagattat
     7021 gcgcaaccag gctgccgaat ccaatcgcgg taatgagtgg accggcaatc ctgatattga
     7081 taacgccatc atcatgctcg accgcataga tacgctggaa aattgcgatg atgaccgtat
     7141 tgaggctgtt aaggctgttt tgcgtagact ggcaggcaac tctccggtaa ctccggatgg
     7201 ttggataagc tgtagtgagc gaatgccccc tcaagatgat tggattttaa tttattcaaa
     7261 gcacggcgag tatatggcag gacaggtaca aggggaatac gtggagttga gcgacggcac
     7321 tttatcgtgg ttagggaacg ccttgttctg gatgcctcta ccagaaccgc cgcaggaggt
     7381 taaccgtggc taacctgcaa cttgccgtta aaggtgaatt tttcgatgcc atgattcgcg
     7441 gagagaaaac ggaagagtat cgcctgtgta atggctactg gaataagcga atcatgttcc
     7501 gggaatatga ccgcctgatt atcacaaagg gatatccgaa gcgcgacgat tccagccgta
     7561 gaattgatgt tccgtatgac ggatatgaaa tcaagacaat cacacatccg cacttcggcg
     7621 ataaaccggt aaaggtgttc gcgataaagg tgaatatcgg caatgaataa caatcctcga
     7681 actcgcgggg atttctttta tctgaactcg ctacggcggg ttttgtttta tggagacaag
     7741 aaatgtcaga tttggctatg aaggttttga aatggcaatc gactggcgat gtcggcatca
     7801 gtagcgcaac tcttgcctca atcgcatgtg gcctgaaaaa gaatatctat ggtcatcact
     7861 tcggcgctcc ccatgacgca gcagactttc ggcgatgcgt tgcacttgtt gagcagattc
     7921 cagaaatcag ggattcattc gacaaggttg caaagcgcgt tccggcattc aaaggaatcc
     7981 tcaacgaatg ggattcactc gttgctctgt tgaagtctga aatgaagacg tacgggaaca
     8041 aagcaccaga gacttacaga agaattagcg aactacgcaa ggactaacca tggaatcaca
     8101 cagcctcaac cccaatgagg catgttaacc ccccctttca ttccagcacc ggagaaaaca
     8161 atgagcgaag ttaacttaat tgtgcccaac gactgggtga cagagcaaaa actgatcgaa
     8221 attacaggtt tacgcccagg aaccatagag agagcacgta aacaatcctg gcttgttggt
     8281 cgtgaatatc tccatgttgc accggacggt attccaaaag aaaacagcga atgcatgtat
     8341 aaccgcaagg ctatcgacca gtgggttgag agtatgtcaa aaaaacagcc tggtgcgcgt
     8401 cgatgaagaa ccgtttatgc ttaatgcgct cttgggcgtc aggagggaac aatggctaag
     8461 acagcatacc caacaggcgt tgagaaccat ggcggatcac tccgcatatg gttcatgtat
     8521 aaaggcacac gagtgcggga aagtctcggt gtgccggata caccaaaaaa caggaaaata
     8581 gctggcgagc tacgagcatc tgtttgtttt tccattaaga cgggtaattt cgactatgcc
     8641 gctcagtttc cggactctcc caacctgaga aaatttggtg cggaaagtaa ggaaattacc
     8701 gtcgcagacc tggctgagaa atggctggat ctgaaaaagc tggaaatcag taccaatgcg
     8761 atggggcgtt acagctccat tgtaagaaat atggttcccc gaatcggagg caacagactt
     8821 gcatctgctg tgactcagga agagctactg ttcatacgaa aggatttgct gaccggatat
     8881 cacgttctga aacgtgggca aaaaacaccc attaagggac gttccgtgcc aactgttaat
     8941 aactacatga acaccatggc ggggatgttt cagttcgcag ccgacagcgg atacatcaaa
     9001 gaaaatccgt tcgcagccat ttcgcaactg aagagatcac gaacggaacc tgatccgtta
     9061 acccgtgatg aatttgtcag gttgattaac gcatttaagc atcagcaatt aaaaaatatg
     9121 tggtcactgg ctgtttatac gggagttcgc cacggagagt tggtttctct ggcatgggaa
     9181 gatatagacc tgaaggctgg gacaatgacg atcagaagaa accacacatt aacgaaagag
     9241 ttcactcttc caaaaaccga agcgggaaca aaccgtgtta tcaaccttat tcaacctgca
     9301 atagacgtgc ttaagaatca ggctgaaatg acacggcttg gtaagcagta tcaggttgaa
     9361 gtgaaacttc gtgagtatgg tcgaactgaa atgcatccgt gcacttttgt tttcaatccg
     9421 caaatcgtta cgcgtaatgg ccttgccggg caccattatg cagttgggtc gattaatcag
     9481 tcatgggaaa ctgcaatgcg acgcgccggg attcgctacc gcaaagcata tcagtccagg
     9541 cacacttatg catgttggtc tttgaccgct ggagctaacc ctaacttcat cgcgaagcaa
     9601 atgggacact ctgatgctca gatggtttat cgcgtttacg gatcgtggat ggcagaaaac
     9661 aatcaggatc aggtatccat tttaaaccag aaattgagtg agtttgcccc atccatgccc
     9721 cacgctgtgg gatcggatgt aattaaacaa gcatagtcaa caggttacaa aacctacacc
     9781 tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
     9841 tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttaacgc cacgccgggt
     9901 tcaccgagag tgaatttttc tgcctgcgtg cgggcggctg tttgtgtatc gcttatgcga
     9961 tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgctgcag tgattcctgc
    10021 gcacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc
    10081 gctgacattt tcatctcccg gataatttct ggtagcaaag cctaccagta agtcaataag
    10141 acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg cggatagaaa aaaacgaaag
    10201 cacaaataat gggagcgtca atttttcgag tttgctgacc cgggagtgag tcttgttcca
    10261 ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc gactgcagcc
    10321 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    10381 gttgccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
    10441 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat cgtcagccaa
    10501 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaagttccg
    10561 gcagataaag ttcacgagct gcgtctgcgc ctggcgcaac aaggtttgcc caaaggcggc
    10621 gcggtcggtt tcgaactgct cgatcaggaa aagtttggta tcagccagtt cagcgaacag
    10681 gtgaattatc agcgggcgct ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg
    10741 gtaaaagggg cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa
    10801 aaatcccctt ctgcatcggt gacggtaaat ctgttacccg gccgcgcact cgatgaaggg
    10861 caaattagcg ccattgtgca tctggtttcc agcgcggtgg ctggtctgcc gccggggaac
    10921 gtcacgctgg tggatcaggg cggacatctg ttaacccagt ccaataccag cgggcgcgat
    10981 cttaatgacg ctcaattgaa atatgccagc gatgtcgaag gccgtattca gcggcgtatt
    11041 gaagcgatcc tgtcgcctat tgttggtaac ggtaatattc acgcccaggt cacggcgcaa
    11101 ctggacttcg ccagtaaaga acaaacagaa gaacagtatc gccctaacgg tgatgaatct
    11161 catgcggcgc ttcgttcacg ccagcttaat gagagcgagc aaagcggttc cggttatccg
    11221 ggcggcgtac cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc
    11281 acgcctccgg caaatcaaaa taaccgccag cagcaggcga gcaccaccag caatagtggg
    11341 ccgcgtagca cacagcggaa tgaaaccagt aactacgaag tcgatcgcac cattcgtcat
    11401 accaaaatga acgtgggcga tgtgcaacgt ctgtcagtcg cggttgtggt gaattacaaa
    11461 accttgccag atggcaaacc gttgcctctc agcaacgaac agatgaagca aattgaagat
    11521 ctgacccgcg aggcgatggg cttttctgaa aaacgcggtg actcgctcaa tgtcgtcaac
    11581 tcgccgttca atagcagtga cgaaagcggc ggagaactgc cgttctggca acagcaagcg
    11641 tttatcgatc agttacttgc tgccggtcgc tggttgctgg tactgctagt ggcgtggctg
    11701 ctgtggcgta aagcggtacg cccgcagcta acacgtcgcg ctgaggcgat gaaagctgta
    11761 cagcaacagg cgcaggcccg cgaggaagtg gaagatgcgg tggaagtccg cctgagcaaa
    11821 gacgaacaac tccaacaacg gcgcgctaac caacgtctgg gggcagaagt catgagccag
    11881 cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg
    11941 ataaataacg atcatgagta acctgacagg taccgataaa agcgtcatcc tgctgatgac
    12001 cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aggtgcaaac
    12061 cctgagcgct gcaatggcga acgtcacgca gatctctaac aagcagctaa ccgatgtgct
    12121 ggcggagttt gagcaagaag ctgaacagtt tgccgcactg aatatcaacg ccaacgatta
    12181 tctgcgttcg gtattggtga aagcccttgg cgaagagcgt gctgccagtc tgctggaaga
    12241 tattctcgaa acccgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca
    12301 gagcgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca
    12361 tctgaagcgc gcccaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga
    12421 cgtgatgttg cgtatcgcta cctttggcgg cgtgcagcct gccgcgctgg cggagctgac
    12481 cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt
    12541 gagaacggct gccgaaatta tcaacctgat gaaaactcag caggaagaag ccgttattac
    12601 cgccgtgcgt gaattcgacg gcgagctggc gcagaaaatc atcgacgaga tgttcctgtt
    12661 cgagaatctg gtggatgtcg acgatcgcag cattcagcgt ctgttgcagg aagtggattc
    12721 cgaatcgctg ttgatcgcgc taaaaggggc cgagcagcca ctgcgcgaga aattcctgcg
    12781 caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgtcaacc gtggtccggt
    12841 gcgtctgtcg caggtggaaa acgaacagaa agcgattctg ctgattgtgc gccgccttgc
    12901 cgaaactggc gagatggtaa ttggcagcgg cgaggatacc tatgtctgat aatctgccct
    12961 ggaaaacctg gacgccggac gatctcgcgc caccacaggc agagtttgtg cccatggtcg
    13021 agccggaaga aaccatcatt gaagagaccg aacccagcct tgagcagcaa ctggcgcaac
    13081 tgcaaatgca ggcccatgag caaggttatc aggcggggat tgccgaaggt cgccagcaag
    13141 gtcatgagca gggctatcag gaaggactgg cccgggggct ggaacaaggt ctggcagagg
    13201 cgaagtctca acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa
    13261 ctacccttga tgcgcttgat agtgtgattg cttcgcgcct gatgcagatg gcgctggagg
    13321 cggcacgtca ggtgatcggt cagacgccaa cggtggataa ctcggcactg atcaaacaga
    13381 tccaacagct gttgcagcaa gaaccgttat tcagcggtaa accacagctg cgcgtgcatc
    13441 cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac cttaagtttg catggctggc
    13501 gcttgcgggg cgatcccacc ctccatcctg gcggctgtaa agtctccgcc gatgaaggcg
    13561 atctcgacgc cagtgtcgcc acccgctggc aagaactctg ccgtctggca gcaccaggag
    13621 tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact ttgaagccaa
    13681 aatggcgcag ttgcctgcgg tacgtcgcta cgggcgatta actcgcgcta ccgggctggt
    13741 gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg agcgccagaa
    13801 cggcagcgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc aacggctgtt
    13861 tttaatgccg ctggaggaag tcgaaggtgt cctgcccggc gcgcgtgtat atgccaaaaa
    13921 catttcggca gaagggctgc aaagcggcaa acagttgccg ctcggtccgg cgttattagg
    13981 tcgcgttctg gacggcagcg gtaaacccct cgatggcctg ccctcccccg atacgacgga
    14041 aaccggtgcg ctgattaccc caccatttaa cccattgcaa cgtacaccga ttgaacatgt
    14101 gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gtcggacgcg ggcagcgtat
    14161 gggtctgttt gctggttctg gtgtcggtaa aagtgtgctg ctggggatga tggcccgtta
    14221 cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga
    14281 tttcattgag aatatcctcg gtgctgaagg gcgtgcacgc tcagtggtga ttgccgctcc
    14341 ggcggatgtg tctccgctcc tgcgaatgca aggtgccgcc tatgccacgc gcattgccga
    14401 agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc
    14461 gatggcccag cgtgaaattg cgctggcgat tggcgaacca cccgccacca aaggttatcc
    14521 accgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggaaatg gcattagcgg
    14581 cggcggctcg attactgcgt tttataccgt gctcaccgag ggcgatgacc agcaggatcc
    14641 gattgccgac tccgcgcggg caatcctcga cggccacatt gtgctgtctc gccgactggc
    14701 ggaagccggg cactatccgg ctatcgatat tgaagcgtcg attagtcgtg caatgacggc
    14761 gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt
    14821 tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct
    14881 cgataaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga
    14941 acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtgtcata
    15001 acccaggaga taacggcaga tggcagaaca tggtgcgctg gcgaccctga aagatctggc
    15061 agaaaaagag gtagaggatg ccgcgcgcct gctgggtgaa atgcgtcgcg gatgtcagca
    15121 ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat gaatatcgca ataacctcaa
    15181 cagcgatatg agtgccggga tgaccagcaa ccgctggatc aactatcagc agtttatcca
    15241 gacgctggaa aaagccatta ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt
    15301 tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact
    15361 gcaggaacgg caatccacgg cggcactgct tgcagaaaac cgcctcgatc agaaaaagat
    15421 ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt agcgcccttg
    15481 attaccgccg acgttgacac caccacattg cctggcggca aagccagcga tgctgcacaa
    15541 gattttctcg cgttgttgag cgaagcatta gcaggcgaga caactaccga caaagcggcc
    15601 ccccagttgc tggtggcaac agataagccc acgacaaaag gcgagccgct gatcagcgat
    15661 attgtttccg acgcgcaaca agcggattta ctgatccctg tggatgaaac accgcctgtc
    15721 atcaacgacg aacaatccac atcaacaccg ttaaccaccg ctcagacgat gacgttggct
    15781 gcggtggctg acaaaaatac gacaaaagac gaaaaagcgg atgatctgaa tgaagacgtc
    15841 accgcaagcc tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg
    15901 actgatgtgc cgtcaaccgt gttaccggca gagaaaccaa cgctcttcac aaaactgact
    15961 tctgcgcaac tcacaacagc acagcctgat gatgcccccg gcacaccagc tcagccatta
    16021 acaccgctgg tagcagaagc ccagagtaaa gcggaagtca tcagcacacc ttcaccggtg
    16081 accgctgccg ccagcccgct aatcactcca caccagacac agccactgcc caccgtcgcc
    16141 gcgcctgttt tgagtgcacc gctgggttct cacgaatggc aacaatcatt aagccagcat
    16201 atttcgctgt tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca cccgcaggat
    16261 ttaggtgaag tgcaaatctc cctcaaagtg gatgataacc aggcgcaaat ccagatggtt
    16321 tcaccacatc agcacgtacg cgccgccctg gaagcagcgc tgccggtact gcgtacgcag
    16381 ctggccgaaa gtggcattca gttagggcaa agcaacatca gtggcgaaag ctttagtggt
    16441 cagcagcagg ccgcttccca gcaacagcaa agccaacgca cagcaaacca tgaacctctg
    16501 gcgggggaag acgacgatac gcttccggtt cccgtctctt tacaagggcg tgtaacaggc
    16561 aacagcggcg ttgatatttt cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc
    16621 cccgctttgt tgcgctcaag acgcaggata attagccgat aagcagtagc gacacaggaa
    16681 gaccgcaaca catgactgat tacgcgataa gcaagaaaag caagcgatcg ctttggatcc
    16741 cgattctggt attcattacc ctcgcggcct gtgccagcgc aggttacagc tactggcatt
    16801 cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt cgtgccctca ccggtcttct
    16861 atgcgctgga tacctttacg gtcaatctgg gcgatgcgga tcgcgtactt tatatcggca
    16921 taacccttcg actcaaagat gaagctaccc gctcgcggct gagtgagtat ttgccggaag
    16981 tccgtagtcg cttgctgtta ctgttttcgc gtcaggatgc tgccgtactg gcgacagaag
    17041 aaggcaagaa aaacctgatt gccgagatta aaaccacact ttccaccccg cttgttgccg
    17101 ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc ttttattctg cgataacgac
    17161 atgggcgata gtattctttc tcaagctgaa attgatgcgc tgttgaatgg cgacagcgaa
    17221 gtcaaagacg aaccgacagc cagtgctagc ggcgaaagtg acattcgtcc gtacgatccg
    17281 aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc
    17341 tttgctcgcc attttcgtat ggggctgttc aacctgctgc gccgtagccc ggatattacc
    17401 gtcggggcca tccgcattca gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc
    17461 aacctgaacc ttatccatct gaaaccgctg cgcggcacag ggctggtggt gttctcgccg
    17521 agtctggtgt ttatcgccgt ggataacctg tttggcggcg atggacgctt cccgaccaaa
    17581 gtggaaggtc gcgagtttac ccataccgaa cagcgcgtca tcaaccgcat gttgaaactg
    17641 gcgcttgaag gctatagcga cgcctggaag gcgattaatc cgctggaagt tgagtacgtg
    17701 cgttcggaaa tgcaggtgaa atttaccaat atcaccacct cgccgaacga cattgtggtt
    17761 aacacgccgt tccatgtgga gattggcaac ctgaccggcg aatttaatat ctgcctgcca
    17821 ttcagcatga tcgagccgct acgggaattg ttggttaacc cgccgctgga aaactcgcgt
    17881 aatgaagatc agaactggcg cgataacctg gtgcgccagg tgcagcattc acagctggag
    17941 ttggtcgcca actttgccga tatctcgcta cgcctgtcgc agattttaaa actgaaaccc
    18001 ggcgacgtcc tgccgataga aaaacccgac cgcatcatcg cccatgttga cggcgtcccg
    18061 gtgctgacca gtcagtacgg cactctcaac ggtcagtatg cgttacggat agaacatttg
    18121 attaacccga ttttaaattc tctgaacgag gaacagccca aatgagtgac atgaataatc
    18181 cggccgatga caacaacggc gcaatggacg atctgtgggc tgaagcgttg agcgaacaaa
    18241 aatcaaccag tagcaaaagc gctgccgacg cggtgttcca gcaatttggc ggtggtgatg
    18301 tcagcggaac gttgcaggat atcgacctga ttatggatat tccggtcaag ctgaccgtcg
    18361 aactgggccg tacgcggatg accatcaaag agctgttgcg tctgacgcaa gggtccgtcg
    18421 tggcgctgga cggtctggcg ggcgaaccac tggatattct gatcaacggt tatttaatcg
    18481 cccagggcga agtggtggtc gttgccgata aatatggcgt gcggatcacc gatatcatta
    18541 ctccgtctga gcgaatgcgt cgcctgagcc gttagtgatg aataaccacg ctactgtgca
    18601 atcttccgcg ccggtttcta ctgcgccact gctgcaggtg agcggcgcac tcatcaccat
    18661 tattgccctg atcctcgctg ctgcctggct gataaaacga ctgggatttg cccctaaacg
    18721 cactggcgtt aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt
    18781 tgtggtggtc gatgtggaag atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa
    18841 tctgctgcat aaacttcccc cttctgcacc aacggaagag ataccgcaga ccgattttca
    18901 gtcggtcatg aaaaatttgc ttaagcgtag cgggagatcc tgatgcgtcg tttattgtct
    18961 gtcgcacctg tccttctctg gctgattacg cccctcgcct tcgcgcaact gccgggtatc
    19021 accagccagc cgctgcctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg
    19081 ttcatcacct cgttgacgtt tattccggca attttactga tgatgaccag tttcacccgc
    19141 atcatcattg tttttggttt attgcgtaac gcgctgggaa caccctccgc gccacctaac
    19201 caggtattgc tggggctggc actgtttttg acctttttta ttatgtcacc ggtgatcgac
    19261 aaaatttatg tcgatgcgta ccagccattt agcgaagaga aaatctcaat gcaggaggcg
    19321 ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg cgaggcggat
    19381 ttagggttgt ttgccagact ggcgaatacc ggcccgttgc agggacctga agccgtgccg
    19441 atgcgcattt tgctcccggc ctacgtgacc agcgagttga aaaccgcatt tcagataggc
    19501 ttcacgattt tcattccttt tttgattatc gacctggtga ttgccagcgt gttgatggca
    19561 ttggggatga tgatggttcc cccagccacc attgctctgc cctttaaact gatgctgttt
    19621 gtactggtgg atggttggca attgctggtc ggttcgctgg cgcagagctt ttacagctag
    19681 agaggcaaaa tgacacctga atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg
    19741 ctggcactgg ctgccccgct gttgctggtg gcgctggtca cgggtcttat catcagtatt
    19801 ttgcaggccg ccacccagat taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc
    19861 gtatttatcg ccattattat tgccggaccg tggatgctca atctgttgct ggattacgtc
    19921 cgcaccttgt tcactaacct gccgtatatc atcgggtagg ccatacaatg atgcaggtga
    19981 caagcgatca atggctttcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg
    20041 cgctcatttc taccgcacca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg
    20101 ggctggcaat gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg
    20161 ttttttcgtt ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg
    20221 gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc
    20281 aaatggggct gtcatttgcg acgtttgtcg atccggccag ccatctcaat atgcccgttt
    20341 tagcgcgtat catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat
    20401 ggttgatttc actgctggtc gatacctttc acaccctgcc tattggtggc gaaccgttga
    20461 acagcaatgc gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga
    20521 tgctggcgtt accgctcatt actctgctgc tgaccctgaa tctggcatta ggtttactga
    20581 atcgtatggc cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg
    20641 gtatctcttt aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttat
//
LOCUS       DAZFAH010000069        20519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFAH010000069 DAZFAH010000000
VERSION     DAZFAH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.069"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1118)
                     /locus_tag="ACHJWB_002383"
     CDS             complement(<1..1118)
                     /locus_tag="ACHJWB_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO4314007.1"
                     /translation="MPVVPTVSGRQVQSRGVQTGGFQTFDVPQAGQVLANVADQYAVA
                     YGEARQKANVALSQDAILQLNQRSNERLYNPQTGFYAQQGKNAIGKGQEYISGFDQDV
                     EEIAASLTDEAARNMFLQQARTQKIQFSTGVLRHEIGQTNAYEDEQYQATRKLWIQNE
                     ADAWNDPQTATLARNSRMVAIARYGAARGWSQERILEEIESDDRSATEMRAKNYAAAN
                     PEGWLNGLFQKNDSGGMDMRAIRLVESGDRHFNPDGSLLEGPITSSGERAQGKYQLMP
                     GTGKELAAKRGVEYNPTDEQQHEMLASDYVNQLYGKYGSEILTGAAYNWGMGNVDKLI
                     AKVGDPRKGEISEEEFIRNLPSETQGWLSRYRKNKTGM"
     gene            complement(1118..1666)
                     /locus_tag="ACHJWB_002384"
     CDS             complement(1118..1666)
                     /locus_tag="ACHJWB_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314008.1"
                     /translation="MCNPAIALVAVTVASTAASMYSQSKQAKYQSAIADRNAEIAEAQ
                     AQDSINRGNIEADQRRREMRQRSGTAAATMGATGAELSSGTALDVFADNAQFGALDAL
                     TTVNNAQREAYGYQVQGMNAQAQGAAAQSAAKSSMTSTLLTAPLKAYGAYQMFGGTWS
                     PFTQSTPAPIGAAAGTRLPGGL"
     gene            complement(1666..2130)
                     /locus_tag="ACHJWB_002385"
     CDS             complement(1666..2130)
                     /locus_tag="ACHJWB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314009.1"
                     /translation="MIDVQIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANGELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            complement(2130..4601)
                     /locus_tag="ACHJWB_002386"
     CDS             complement(2130..4601)
                     /locus_tag="ACHJWB_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314010.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTASASIFGAEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTGTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGEDNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRAEYTISVSGGAYFTSSDVGAQLQFPNTGTDPDI
                     GDEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNTATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITAEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VKIRQLDPLPLSVLAVIPRLTVGGF"
     gene            complement(4601..5206)
                     /locus_tag="ACHJWB_002387"
     CDS             complement(4601..5206)
                     /locus_tag="ACHJWB_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314011.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTNGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNRVILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            complement(5206..5529)
                     /gene="gp10"
                     /locus_tag="ACHJWB_002388"
     CDS             complement(5206..5529)
                     /gene="gp10"
                     /locus_tag="ACHJWB_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid staple protein"
                     /protein_id="HFO4314012.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPQGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            complement(5580..5915)
                     /locus_tag="ACHJWB_002389"
     CDS             complement(5580..5915)
                     /locus_tag="ACHJWB_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314013.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            complement(5926..6363)
                     /locus_tag="ACHJWB_002390"
     CDS             complement(5926..6363)
                     /locus_tag="ACHJWB_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bbp16 family capsid cement protein"
                     /protein_id="HFO4314014.1"
                     /translation="MITDKLLMFSEAQAVTDTAASTDVIDLGPIDGNRRDIGVGYPLE
                     FWVLVNEAATASGEATVNIQLQTSENNSSWSTIYDSGALAKATLTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGKFTAGISLDVDANTPYPIRSKVTG"
     gene            complement(6415..7401)
                     /locus_tag="ACHJWB_002391"
     CDS             complement(6415..7401)
                     /locus_tag="ACHJWB_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4314015.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLSQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSIGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQAMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDANGGKYEGYRTH
                     YKWDNGLTLRDWRYVVRIANIDVSNLDAPSTAANIAKLMIRALHRIPNRGMGRPVFYM
                     NRTINEVLDLQSLEKSSLAISVKETEGEWWTALRGVPIREIDALLETEARVV"
     gene            complement(7416..8111)
                     /locus_tag="ACHJWB_002392"
     CDS             complement(7416..8111)
                     /locus_tag="ACHJWB_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO4314016.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPAGDNPAPVGDPS
                     QQEGDKPQPVADGDKPADDKKPENDKQDEKKDGDKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            complement(8114..8410)
                     /locus_tag="ACHJWB_002393"
     CDS             complement(8114..8410)
                     /locus_tag="ACHJWB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314017.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            complement(8407..10086)
                     /locus_tag="ACHJWB_002394"
     CDS             complement(8407..10086)
                     /locus_tag="ACHJWB_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO4314018.1"
                     /translation="MAETTKERLNKQFAQLESERQSFEPHWRELSDYINPRGSRFLTS
                     EANRNDRRNTRIIDSTGTMAARTLASGMMSGITSPARPWFRLATPDPEMMDYGPVKLW
                     LEAVQNRMNDMFNKSNLYQSLPQLYGSLGTYSTGAMAVLEDDEDIIRTMPFPIGSYYL
                     ANSPRGSVDTCFRKFSMTVRQLVQEFGLNNVSESVKSMWESGTYEKWIEVMHSVYPNI
                     DRDTSKLDSKNKPFKSVYYEVGGDNDKLLRESGFDEFPIMAPRWEVNGEDVYGSSCPG
                     MLALGPVKALQLLQKRKSQLIDKATNPPMVAPTSLKNQRASLLPGDITYIDQITGQDG
                     FRPAYLVNPSTADLVADIQDTRQIINSAYFVDLFMMLQNINTRSMPVEAVIEMKEEKL
                     LMLGPVLERLNDECLNPLIDRAFSMMVRKNMLPPPPDAMEGMPLKVEYISVMAQAQKS
                     IGLSSLASTVNFIGQLAQAKPEALDKLNVDQAIDAFADMSGVSPTVIVPQEQVEQARQ
                     QRAQQQQQQQMMAMGMAAAQGAKTLSEAKTSDPSVLSAMANAVSGQGGQSQ"
     gene            complement(10101..10307)
                     /locus_tag="ACHJWB_002395"
     CDS             complement(10101..10307)
                     /locus_tag="ACHJWB_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314019.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            10735..10811
                     /locus_tag="ACHJWB_002396"
     tRNA            10735..10811
                     /locus_tag="ACHJWB_002396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10769..10771,aa:Arg,seq:tct)
     gene            11034..11852
                     /locus_tag="ACHJWB_002397"
     CDS             11034..11852
                     /locus_tag="ACHJWB_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314020.1"
                     /translation="MKNFKVISMFESFKELFLSTANTAVNRAKNPVLGAFVMSWCAFN
                     WKSILYLFFSKSNIIDKISYISDNSTWKTVMFYPCLSVIAICCLLPWVNNIINVWQAK
                     PLDNNDSIENHLKARKIQRETRLQRLLAKKDVTYDKVKTGAEKDIQEMKEEIIRSKNS
                     MGELTAELKAKDDELRSASAQLAALNHSLKEISETLGRMNEAYKTLQNDFDEYKLKYP
                     EKSQIKSLALGNGQTISNVLEQHNLSGLKSGKPNVFNNFGVLSGLSGFEDKNKD"
     gene            complement(11849..13324)
                     /locus_tag="ACHJWB_002398"
     CDS             complement(11849..13324)
                     /locus_tag="ACHJWB_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4314021.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRQWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKDWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNSCKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRLPGQQNQQGKALTEYDPYA"
     gene            complement(13321..13995)
                     /locus_tag="ACHJWB_002399"
     CDS             complement(13321..13995)
                     /locus_tag="ACHJWB_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4314022.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEPKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLELIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            complement(14036..14374)
                     /locus_tag="ACHJWB_002400"
     CDS             complement(14036..14374)
                     /locus_tag="ACHJWB_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314023.1"
                     /translation="MAKSPAERKAAQRARQSAAGERKIELVLDKQEQEMLARNCATRR
                     PGRAPYEFGEYIALLIRQDDARVRGRIKSISRKCCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            complement(14367..14489)
                     /locus_tag="ACHJWB_002401"
     CDS             complement(14367..14489)
                     /locus_tag="ACHJWB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314024.1"
                     /translation="MNKCNALLYAMVIGFGLAAGIRVYITWESLINLAWSAIRG"
     gene            complement(14489..14893)
                     /locus_tag="ACHJWB_002402"
                     /pseudo
     CDS             complement(14489..14893)
                     /locus_tag="ACHJWB_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(15049..15573)
                     /locus_tag="ACHJWB_002403"
     CDS             complement(15049..15573)
                     /locus_tag="ACHJWB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314025.1"
                     /translation="MLTAFKIALASLEADPIGEVSEKRYGLIMDGTVDLGGESTYRII
                     KGEKAMKLFPLGTKFYTAPPAPIALEAIENAIEYIRSIAFHIDEDDYHGKHIAYFMRQ
                     ALAWLEGHSCSADSQGKSDNSPLPRYQVIELTVLVKQLVSQLKKAKPDCKLPDRAMDY
                     LLRNALVSAEDVLR"
     gene            complement(15675..15866)
                     /locus_tag="ACHJWB_002404"
     CDS             complement(15675..15866)
                     /locus_tag="ACHJWB_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314026.1"
                     /translation="MNGQISIVRPGACDDCEIRMIIRLAMGRTITALITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            complement(15868..16584)
                     /locus_tag="ACHJWB_002405"
     CDS             complement(15868..16584)
                     /locus_tag="ACHJWB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4314027.1"
                     /translation="MSKIDYQALREAAEKATCGEWSLEYGESRFDGDDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGRLRVELEGKDSKIANLTAERDALREGEMGDARHSNTRAAADIYFQLVEE
                     CEIPAGGSLVEYVDDMREKLESAEKRIAELEDREILLPERSSMLHRTDFHDDYQTVMA
                     YKVSEVIDAIRAAGIRIKGV"
     gene            complement(16581..16991)
                     /locus_tag="ACHJWB_002406"
     CDS             complement(16581..16991)
                     /locus_tag="ACHJWB_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314028.1"
                     /translation="MDGNIKGLVSAGHEMASELKAECGAVDMRSVAKLISDLATQLEV
                     QLARANALAAENAGLKKFCKDAAFDADYEAELGMERGGFSDALNEIKTPATDAFLAEV
                     RAQGVEMFADHLLCPDLDDTIRDFAAQLRKGGNQ"
     gene            complement(17053..17400)
                     /locus_tag="ACHJWB_002407"
     CDS             complement(17053..17400)
                     /locus_tag="ACHJWB_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFO4314029.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLEQRKRAGEVAWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGGFWTDDARVKTKVAADQYPFRIIGVTVK
                     PKKAGGGWNIEEF"
     gene            complement(17518..18303)
                     /locus_tag="ACHJWB_002408"
     CDS             complement(17518..18303)
                     /locus_tag="ACHJWB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4314030.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYTNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKPEPMSTPPDTG
                     RDVYHPPGRSFGCMPNAATLGGITPAQWLMEEYRRGKAAGFIK"
     gene            complement(18300..19115)
                     /locus_tag="ACHJWB_002409"
     CDS             complement(18300..19115)
                     /locus_tag="ACHJWB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4314031.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLV
                     KDTCLNKKTVQAGLISLMKMGLISDIGDRKGATKRVRVFSLNITKNGNIKGNREGGNE
                     PENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKNGDSVHH
                     DPGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAE
                     FRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWNSPEAWEDFL"
     gene            complement(19131..19331)
                     /locus_tag="ACHJWB_002410"
     CDS             complement(19131..19331)
                     /locus_tag="ACHJWB_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314032.1"
                     /translation="MEIITRIDAAKRGLKRYYTGKPCKHGHDSERWVYNGHCVECTME
                     SNRRIRAEIKQIMINSSPQHSS"
     gene            complement(19482..19712)
                     /locus_tag="ACHJWB_002411"
     CDS             complement(19482..19712)
                     /locus_tag="ACHJWB_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314033.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            19867..20451
                     /locus_tag="ACHJWB_002412"
     CDS             19867..20451
                     /locus_tag="ACHJWB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4314034.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKSKQ"
BASE COUNT         4850 a         5429 c         4703 g         5537 t
ORIGIN      
        1 tccattccag ttttattttt tctatatcgg gaaagccacc cttgtgtttc tgatggaaga
       61 tttcggataa attcttcttc tgatatttca cctttacgtg gatcaccgac tttggcgatc
      121 agtttatcca cgttacccat cccccagtta tatgctgctc cggtcaatat ttcggaaccg
      181 tacttaccat acagttgatt tacatagtca ctggcgagca tttcatgctg ttgttcgtcc
      241 gtagggttgt attcaacgcc acgcttggcc gccagttctt ttcctgtgcc cggcattaac
      301 tggtatttcc cctgggctct ctctccagaa gatgttatcg gtccctcaag aagactacca
      361 tcaggattaa aatgtcgatc acctgattca acaaggcgta tggcacgcat gtccatgcct
      421 ccagaatcat ttttctgaaa cagaccattt agccatcctt ctggattggc agcggcataa
      481 ttcttcgccc gcatttctgt ggcactgcga tcatcacttt ctatttcttc cagaatgcgt
      541 tcttgtgacc atcccctggc agctccatat ctggcaatgg ctaccattct ggaatttctg
      601 gctaaagtgg cagtttgcgg gtcattccag gcatccgctt cattttgtat ccataatttt
      661 ctcgttgcct gatattgctc atcttcatag gcatttgtct gccctatctc atgtctgaga
      721 actccagtac tgaactgaat tttctgtgtt ctggcttgtt gcaaaaacat atttcttgca
      781 gcttcatcag tcaatgaagc agctatttct tccacatcct gatcaaatcc agatatgtac
      841 tcttgcccct taccaatcgc atttttgcct tgttgtgcat aaaaaccggt ttgagggtta
      901 taaagacgtt cattgctgcg ctgattaagc tgaaggatgg catcctgaga caatgcaaca
      961 ttcgctttct gcctggcttc accatatgcc accgcatact gatctgcgac attcgccagc
     1021 acctgacctg cttgaggaac atcgaaggtc tgaaaaccac cggtttgcac accacgactt
     1081 tgcacctgac gtccggatac tgttggtacg actggcatta taatcctccg ggtaatctgg
     1141 ttcctgctgc tgccccgatt ggcgcagggg tgctttgagt aaacggactc cacgtcccac
     1201 caaacatctg gtatgcaccg tatgctttta gtggtgccgt taacaaagtg ctggtcatcg
     1261 atgatttagc agccgactga gcagcagccc cctgtgcctg agcattcatt ccctgaacct
     1321 gatacccata tgcctcacgc tgagcattat tcactgtcgt taacgcatca agagcgccga
     1381 actgagcatt atccgcaaaa acgtcaagag ctgttccgct acttaattcc gcaccggtag
     1441 cccccatagt ggccgccgca gtgcctgagc gttgacgcat ttcacgacga cgctgatccg
     1501 cttcaatatt cccacgattg attgaatcct gtgcctgagc ttcagcaatt tcagcattcc
     1561 gatcagctat ggctgactgg tattttgcct gcttgctctg gctgtacatt gacgcggctg
     1621 tggatgccac tgtgacggca accaaagcga tggctgggtt acacattatt ttctctccat
     1681 gtgaaatctg tggaaattaa gaccaagagc accataaggc gcggcttctt caagcctgaa
     1741 tccaagccag tgcagccatg ccttggcaac atggtttcgc tcgtcgacgt agttttccag
     1801 gcgcggataa actgccagca tctgctgcaa tacagggcgg cagtggcgaa gaaatgtctt
     1861 ctgatatttt tcaatacggc tggttcctac cagccagggc gtaccattgc caccgatcat
     1921 tgacgccgga gatacaccaa acatggttac cagttctccg ttcgcgaacc ctgaccaggc
     1981 catagtcgca gtgcgcagac caacacgcag cgcatcttcg gtagtcatca gcgataccgc
     2041 atacagttcg tcaatatcag cctgacgaac atccggcaaa atcatctgaa gatgctcttc
     2101 ggttgcggga ataatttgaa catcgatcat cagaatcccc caacagtaag gcgaggaata
     2161 acggcaagaa cagacagcgg caacggatca agctgacgga tttttacacg tccgtttttg
     2221 ccccagttac tgtccagttt cacttctact tttccggtag cgtcatcaac aggatcatcg
     2281 tagaactcga attcacgctg tggatattcg taccatttac cgccgggggt agtcgcccag
     2341 atgccgcgac tggcattcac aaccagagta acggagggga tcacctgttt tttgtccagc
     2401 agcgtttcct gtccgttaat gttgatatcc agcgtttcga attcagcagt tattggcagg
     2461 ccgatgtgca ctacagcccc cggtgattcc agcgtgacgg cacctccgga aaccactttc
     2521 tgtggttcca cgttggcatc agagagaata tttacggtct ggccttcaag atgagacaag
     2581 ccgccaaatg tccggcgcgc catctgccag ttcgtggtgg ccgtattcct gagggatggc
     2641 gggacgttcc tgttagcacg aaccactaca gcagtattgc tggttacaga aataatgtcg
     2701 caacgtaatt cttttgacac ttcatcgcca atatcaggat cggttccggt attagggaac
     2761 tgtagttgcg cgccgacatc actactggtg aagtacgcgc caccagaaac actgattgta
     2821 tattccgcac ggtaatccca ttcaccagaa ccaccagtga tggtcatcgt tctgtcagac
     2881 gtatttcttc catcatagct aaggccagaa tcaacaaaga aagcatcttc atcgctggta
     2941 aataaacggc tggacagtct ctcgatgtat ctcactgttt gcccgttaac ggttctgtta
     3001 acgacgaaat acaccgcatc ttcattgcct tcgctgatac tgcatgtgct ttcatatttt
     3061 ccggtactgg attgtggtgc ccatgcaaaa acctgctgat cacgcaaata ggtcatcacc
     3121 agtaatttac cgtcatcacg aatgcagaag gcactggagt aagggacaat cgagaagcac
     3181 cagtcaacaa tgctgtgctt ctgaaaaaga tgattggcaa ggatggtcag gtcgttcccc
     3241 tgatagccgt caacatcgaa tgagtaggcc agatcacgga caacgctgcc tttctcctgg
     3301 acgaacagag caatattcgc cacggcaatt ggcgggacgt tgcttgagcc atttgatccc
     3361 tgagagctga atgcaaatga tgatggggtt aacactttgt tctggtcgcc ggtgatgacg
     3421 tactcacctc cggaagtcag cgccaccagc gaaccaacat caatcaggtg acggatctca
     3481 ttaacctgac gcccggcata ggtgtagata attctgtcgt catcctgcgt aggattgctt
     3541 ttgccaaaat ccttataatc cccggtacgg ctggcccaga tagtctgagg gaacgcagtc
     3601 gatgcggcga agtaaagacg ttgttgataa taaacaacag tgccaggata accattaaca
     3661 ctgttccagg catatttagc ccatttatag ctggcattat cctcgccaac tacctgcgaa
     3721 gggatatagg aaatcacctc ggcagttgca gtagttccat ttgcagcagt gatacgggca
     3781 atgccaaaac cactgtgcag atattcccac tcaatgccgg tatcatcatc accggatccg
     3841 ccccagccat cccatgatgt gccttctgta tgcgaagggc gcaaagtacc tgttttgcct
     3901 gctgtaacgg cgcgatagta gttactgtct gcacggcgaa tatcgccaat cgacgtactc
     3961 ttactggttt cccataccgg cactgaatca actgcaggct gttccagata gaacaatttg
     4021 cctacctgct ccgcgccaaa aatagaggcg cttgccgtta acgtaattgt cccggtgctg
     4081 gcgctggcat aaaccgtcac tgactcgtca atattgatat cttcaaatgg tccgttcttc
     4141 gttaccacat caaccagttg ccagttgtca tgcgcatagc gacgcaactc tttcggcggg
     4201 tatgccggat gaacaagcgt aagcacgtct gcgctttgcg tgaatttaat tcggaacaga
     4261 tcggcttcag tatatggcgt ggcaatttca taaataacat tgctgctgtt cagcaccaac
     4321 gcaccatctt tgataacgcg catgtactgg tgtccgaact ccagagcata agtctgaacc
     4381 gtcgagaact ggaacggtat caggcggcat ttccgatttg ggtatttggc ggcaccgaca
     4441 aaacgcgtac caggtcgatt ctcaacgccg ccatactgcc gcacgataaa gttatcgcac
     4501 ttgcgcaatg ctacctggta cttcgccatg tcgatacgcc cgtacaacga cggtccaatc
     4561 tcaccaccgg caaagctggg ctggatccaa ctgatagcca tcaggacaac ctcgcaatgg
     4621 taaactcgtc aaccggtggc tgtggttcct gtgattcatt ctggctatgc gaaccagcac
     4681 taagaatcac gcgattgtac atattgagag caaatgtacc gagatccgca ttcccagtca
     4741 gcgccatgtt aatggctgcc gcaagacgcc aggccagcgc ctccataaaa atggcatcaa
     4801 acatgttcac atctgaaacg cgagagacat acttgagcca tgcctgcggc tggtctgtat
     4861 agatcaattt tcctgttccg ttggtgtctg caccaacttc gtactgaacg cgcattgctg
     4921 ctgttggatt gcgtacacca ggaagcataa tttcagtaat gcgcagacaa tcggacgggt
     4981 actggtacgc atattcccag tcaggcggtg gattgctcgt atctgcaagc gccacgcgtt
     5041 tggtagcaaa gttccagtca aaatcagaaa gcacagcatc acggcaggcc tcaaagtgca
     5101 gcgaacattc ccccgcttcc ttgctggctt ccgtcaggct gttaatgctg cggctattgc
     5161 caatattgga cagcgcacga ttacagatct ctactacaga ggccatcact cacctccgtt
     5221 accgtacaga gtttcagccg ctgatttttc tacatccccg gaaacaggag cgatcgccat
     5281 atcagtgatc tgcagatcgg cgctgcgatt aacaccatcg tcagtttctc tggcagacag
     5341 gcctcgaata acagcctttg cagttatcat cacttctgtt ccgacgccct gaggttgcgc
     5401 cttcagctta ttcaatgtgt cgttattaag agtgatgcac agcccccacg ggtattcatc
     5461 gcgagttctg gtttctccgc tctcatcctg gtagctgtca gtgccggttt tgaggtttac
     5521 gagttccata tacactcctg caataaaggg gccgaagccc cttgtcggat tcgcgaggct
     5581 tacacgccca gttctttacg cttatctgcg atcttctcgc ggagcgtttc ggctttagcg
     5641 ttatggtgtg gcttctcgtt aaagagcaat tcgtactctt cacggagctt atccagttca
     5701 ccatcatctg acacatcgtt gatgattttg gtgctggttg ctgccataga cacctttcct
     5761 gctacctttg cttttgcctg tctggctgca tcgttaacag gttccagtgc gctaccaggc
     5821 tcaccttcgt attcgatttc tgccccctcc ggccacagtg tgttatggat atgagagagg
     5881 cgcagaacgc ggtatcttgg tttctcacct gacatcgata tcaccttaac cagttacttt
     5941 tgagcggatc gggtacggcg tattggcatc aacatccaga ctgatacccg cagtgaattt
     6001 gccggccgtt agtgggccag ttgcgacgga gtagttaaca cgcagatatc gctgaacacc
     6061 ggcaggcacc tttgcagaaa caactcgttt acctgctgtc agggtagcct ttgccagtgc
     6121 gccactatca taaatagtgg accatgagct gttattctca ctcgtctgca actggatgtt
     6181 tacagttgcc tcaccacttg ccgtggcggc ttcgttaacc agcacccaaa actcaagcgg
     6241 gtaacccaca ccgatatcgc gacggtttcc gtcaattgga ccgagatcga ttacgtcagt
     6301 agaagccgcg gtatcagtta ccgcctgtgc ttcggagaac atcaacagtt tgtcggtgat
     6361 catcttcttt ctccattagt gggcctgttg cggcccacag gttaataaca ggcgttacac
     6421 cacgcgggct tctgtttcca gaagtgcgtc aatttcgcga atcggcacac cacgaagtgc
     6481 tgtccaccat tcgccttctg tctctttcac gctgatcgcc agagaactct tctccagaga
     6541 ttgcaggtca agcacttcgt taatggttct gttcatgtag aaaacaggac gtcccatccc
     6601 acgatttggg attcgatgaa gggcacgaat catcagtttt gcgatattcg cggcagtaga
     6661 aggagcgtca agattactga catcaatgtt tgcgatacga actacatagc gccagtcacg
     6721 taatgtcagc ccgttgtccc acttataatg ggtacgataa ccttcgtact tgccgccatt
     6781 agcatcttcc agtgtcacct ggcctttatc ttccatctga atgccagcct tctgtccttt
     6841 cgggaagatg ccatgcacgg tgttttcgcc ccacaccact aaccagattg aagtgttatc
     6901 tgtacccgtg ccaccagcat caatgatgtt ctgagcatta cccgcagaca ggctggaata
     6961 tcgggaggac agtcccataa actgctgagg gttaacgctg gaatcaccgt aaaacagtgt
     7021 ctgcgccatc gcctgattca tcgcttcaat aaatgctcgg tcttcagaca gacggaattc
     7081 ggcagtatta ccgttcagat cagccagaga cttatcgact tcagcatagg tttccagcat
     7141 gccaatggaa tcggtgacct gcactgtggt tgatttgctt ggctgtacgc catagttcag
     7201 caaacgccag gtagcggaag gtaaaccaga acgaatggtg gttcggtgtc cggtaggaag
     7261 gtttccttca acaaaaggca tatcctgaag aattgggtta gtttgactga gaagctcgat
     7321 aatcttatcg actttcccgt ttggatcgac gcgcttaccc cagtcagcca gcgttagcgc
     7381 agttaagcct ttaacagcca ttgtcatttc ctctcttatt tgccatagag cacttcggcc
     7441 gcactacgct ggccttcatt accaccggtg accatgccat cttcagacat cgcctttccg
     7501 attttcacga acgttttgac cagatcaggg tgattaccca gcccggtggt gttcagatat
     7561 tctttgagtt caggtgtccc gaactggtca agcgcacgct gtgcggcgct aaggttagaa
     7621 atcaacttgt cgccaccgat ttctttgtcg gcttttacat ccgcagccca ctgctcggtt
     7681 gttttctgcc aggcttctgc ctggcgctgc tgaacacctg ccagaatctt cggataagca
     7741 tcaaccagct tttgcgcttg ctcgttggtc aggtttagtt ctcgcgccac cggctcgaat
     7801 tccttcaacg cttctgtatc cagctctacg ccttcggcag cctgaaactc gtacttttca
     7861 ggcgcaccct ctggtttatc gccgtccttt ttttcatcct gcttatcgtt ttcaggcttt
     7921 ttgtcatcag caggtttatc gccatcagca acaggttgtg gcttatcacc ttcctgttgt
     7981 gatggatcac caactggagc agggttatca cctgcaggcg ctgacggttc tgacgcagcc
     8041 ggagctgctc caccatcgac tggttgctca ttgcaaagac ggcgatacag caaacgctca
     8101 aataaattca tgatcactcc tgttcactgg cctctttggc catcttcaaa tactgttcag
     8161 ggcaatgcgc cataacgcgc tgaaacagtt ccagcgccag attgcgttgc ccctcattaa
     8221 atgccattgc catagcgtcc atcggtgaga tagcggaaaa cacacggcct ttctccagca
     8281 ccgaccagac aacgcgacgc ccctgttcac tgctcatgac aaagcgaatg tcatcaattt
     8341 cacgctgcgc catgtcacgt tgcttacggg cgttttcttc tttcagttga tcgtcttcgt
     8401 aatctgtcat tgtgattgcc caccctgacc actaactgca ttcgccatag ctgacaaaac
     8461 actcggatcc gaagttttag cttcgcttag tgtcttggca ccctgtgccg ccgccatccc
     8521 catcgccatc atttgttgct gctgttgctg ctgtgcccgt tgctggcgag cctgctcaac
     8581 ctgttcctgc ggaacaatga cggttggaga cactccggac atatcagcga atgcatcgat
     8641 cgcctgatca acgttgagtt tgtcgagagc ttctggtttc gcttgcgcaa gttgaccaat
     8701 gaagttaacc gtggacgcca gactggacag gccgatagac ttctgcgcct gagccatgac
     8761 ggaaatgtat tcgaccttca ggggcatacc ttccatcgcg tcaggcggtg gcggcagcat
     8821 gtttttacgc accatcatcg agaaagcgcg gtcaatgaga gggttaagac attcgtcgtt
     8881 cagacgctcc agaaccggcc ccaacatcag aagcttttct tctttcattt cgatcactgc
     8941 ttcaacaggc atcgagcggg tattgatgtt ctgcaacatc atgaacagat cgacaaagta
     9001 ggcgctgtta atgatttggc gggtgtcctg aatgtctgct accagatctg ctgtactggg
     9061 gttaaccaga taagcaggcc tgaaaccatc ctgaccagta atctgatcga tatacgtgat
     9121 gtcgccagga agaagagagg cgcgctgatt cttgagggaa gtcggagcaa ccatcggcgg
     9181 attggtggct ttatcaatca actgcgactt gcgcttctgg agaagctgca atgccttaac
     9241 aggtccaagc gccagcatac ccgggcatga tgatccataa acatcttcgc cgttaacttc
     9301 ccagcgcgga gccataattg gaaactcatc gaatccggac tcacgcaaca acttgtcgtt
     9361 atcgccacca acctcgtaat aaaccgattt gaatggcttg ttcttgctat ccagcttcga
     9421 tgtatcgcgg tcaatgttcg ggtaaaccga atgcatcact tcaatccact tctcgtaggt
     9481 gccgctttcc cacatgcttt ttacggattc gctgacgtta tttagtccga actcctgaac
     9541 aagctgacga acagtcatag agaacttgcg aaaacaggtg tccacactgc cacgaggtga
     9601 gttagccagg tagtaacttc ctatcgggaa tggcattgta cgaatgatgt cctcgtcatc
     9661 ctccagcacc gccattgcgc cagtgctgta tgtgccgagg cttccgtata actgcggcag
     9721 cgactgatag agattcgact tattgaacat atcgttcatg cggttctgaa ctgcctcaag
     9781 ccacaactta acagggccat aatccatcat ttcaggatct ggcgtagcca ggcgaaacca
     9841 cggacgcgcg gggcttgtga tgcctgacat catgccgctg gcgagagtgc gcgccgccat
     9901 agtcccggtc gaatcaataa tgcgtgtatt gcgccgatcg ttacggttgg cctcagaagt
     9961 cagaaagcgg gaaccacgcg ggttgatgta atcactcaac tcgcgccagt gcggctcgaa
    10021 cgactgacgc tcgctttcaa gttgtgcgaa ctgtttgttc aatcgctctt tagttgtttc
    10081 cgccatttca atgactccgg ttactgacca agcagcgttt taccgctggt attagcggtt
    10141 gatgtgtcgc cctgagaacc ggtaagcagc gtagaactac gaccagcagc agcgcgacgg
    10201 cgacgagttt cttcgtcgcg ggcatcaaca acggcggcat cctgctcctg tggtgctgcc
    10261 tgaacttctg gtgttgcagg cactgatggt gagctaccca tgcacatatc aatgactccg
    10321 tacgcaatta aattattacc aatttaacca catatgattt atttatcgta gatagttgac
    10381 atttaacgca caaattatta cctttcaggt aaccaaagag ttcattccgg ttactaacct
    10441 gactggcttg tcgttaaatt gaacaggtgg agtgagcttt tattttgagc agtacggcgt
    10501 atggcacatg cgccgatagc ggtctggata cgtttaaggg gcaccctccc ttgctcgggc
    10561 aaacgaacca ggtagccgga atgtgcaagt cgagcggttt tattccgcgc acggggattc
    10621 accatcccgg cgattcggtg tgacgcctcg gaagagacga gggtacaacg atgagagcat
    10681 ttatggagcc gcgacaaagt gtggcgcctt aacaggctaa gtgctctcag cgttgtggca
    10741 ttagctcagt tggacagagc aaccgccttc taagcggttg gtcgcaggtt cgaatcctgc
    10801 atgccacgcc agaatcacgc ctaaggaccg tgatgccaga agttccaggg gcttggcggt
    10861 gatggtttcc cttgaaggac tatcaccgcc ctttttacag caggacgcca ttgcgatgac
    10921 ttcatgctgt aaaccagtac agccacggaa ggcataactc attgcttcca gttcgcccgg
    10981 ttcgccgggc atttttttga ttgatgaccg caaattacct taaaggtata atcatgaaaa
    11041 acttcaaggt aattagcatg tttgaatcgt tcaaagagct gtttttatct actgccaata
    11101 ctgccgtgaa tcgagccaaa aaccctgtgc ttggtgcttt tgttatgtcc tggtgcgcct
    11161 tcaactggaa atcaattctt tatctatttt ttagcaaatc aaacataata gataaaattt
    11221 catatatctc agataacagc acatggaaaa ctgttatgtt ttatccatgc ttatctgtaa
    11281 ttgctatctg ctgcctatta ccatgggtaa acaatatcat taacgtatgg caagcaaagc
    11341 ctcttgataa taatgactca atcgaaaatc acctgaaggc aagaaaaatc cagcgtgaaa
    11401 caagactgca gaggttattg gctaaaaaag atgttacata cgacaaagtt aagactggcg
    11461 cggaaaaaga catccaagag atgaaagaag agattattcg atcaaagaat agtatggggg
    11521 aattgactgc cgagttgaaa gctaaagatg acgaattgag atccgccagt gcacagttag
    11581 cagctctaaa tcattcatta aaagagatat cggaaactct tggaagaatg aatgaggcat
    11641 ataagaccct ccaaaatgat ttcgacgaat acaaactcaa ataccctgaa aaatctcaaa
    11701 taaaaagcct tgcacttggc aacggccaaa caattagcaa cgttttggag caacacaact
    11761 tatccggatt aaaatctggt aagccaaacg tttttaataa ctttggtgtt ctatctggat
    11821 tatcaggttt cgaggataaa aataaagact aagcatatgg atcgtactcg gtaagcgcct
    11881 tgccttgctg gttctgctgc ccgggaagtc gcaggcgctt tgacaccggg aaagcaaacg
    11941 tcagcagtag cgcatcgcct ttaccaggcg aacgcccaag tcgttctttg atatcttcct
    12001 tcggttcgat aacgatttta ccgtccacgc gaactttgta ctctgccgcc gacagatcgt
    12061 ccgctgtttc ctggtcatcc agcatcccgc ccaacctcag ccatgtcttg catgagttga
    12121 acatctcccc gcgcttgtta agcatctgcg ggtcagtgga cgcaccgccg aacggaacaa
    12181 gttgccatgt acgcccccag ccatcaccga ttgacttcaa accggttccg taaccgaagt
    12241 cgatgaacac cgcgtcagcc tgatactggt cttcaaagtc agcgatacgc ttcgccataa
    12301 tcagatcgtc ggtagtcttg ttgccagtcc atagcacctt actatgcaac ccctgccgca
    12361 ggtatatcac cgcgtcatca acgcctgaat atgccgggtc aacaccgatt atcaccggag
    12421 catgtgccac ctgcgcagcg gttaccaccc gtttcattgc ctcatcagta agaccggtag
    12481 ggataaactg caattcagat gcatcaggga atatgccgcg cacacggatt ttaacgaagt
    12541 cgctgtcttc cccgtagtca tcaacccatt tctgcaactg ctgtttgtta gtaccttcca
    12601 ccgtccggct gtcaatctgc gcagttttcc agcggtgttt gtatttgcgg aaacattcgc
    12661 gaaaacgccc ggtattacgt gtagggtttc cgaacgccac ccagataatc tcagtgtctt
    12721 cgtccgtaag cgcaccctca gcaacttccc acaccagatc cgcaatgttc gacgcttcat
    12781 cgaataccac gatgatgcgt ttgcgctcgt tgtgtagtcc ggcgaatgcc tcagtgttgt
    12841 gctcagacca ggggattgcg tcagctcgcc accgcttgtc gtgcccagga tcattgctgt
    12901 acatcgcagt agcggtacag gtaaaccagt ctttcgtgat agcaaggttc gaccacttga
    12961 taatttccgg ccaggtcttc gttcgtagct ggttgtcggt gttggcggtc accacgacct
    13021 tacaatcctc gcaagtggac atgccccagt tgatcagcat tgagatgaat gcggatttac
    13081 caataccatg accagaagcg cgtgccagca taagcggctg atagcgcgtc tctggattct
    13141 gcaggtgatc acgtatctct cggaacgcat cagcctgcca ctgacgtggg ccggtagcat
    13201 gtgccagttc agtcccctct tccccccacg ggaacgcata tagggcatag ccaagcggat
    13261 cgtgagtgaa ccctgcaata tcctcgatca actgctcttc aggagataac gctgtatctg
    13321 tcactgatta ccatcctgac gttctttgag tcgcttcctg gctgctgcta tgcgatcagc
    13381 aattgtcaca ttcacattaa catccagacg ttctttgaac gcgttgacat caacatgctt
    13441 accaatcagc tcaaggttct tcaccttgtc aggccattta atttttttga ggattgtctc
    13501 tatcgaatcc tcgttcatgt tcatgatggt cgatgacaga tcaaagccac taagcgtagt
    13561 gcgccagatt ttcggccact cacggattgg tttaaggctc ccatcgtcgt tgaggatgtc
    13621 gatcacgtcc atctggtcga tctccaccag gcgcatgaga acgtaatcag cactgacgcg
    13681 cattcgtttg ttgcgctcct ccatcaactc ggcaatccgt ttttgaatgc gttcatcgcg
    13741 catcatgaca ctggctttaa ctgccgctgt atttggggag aatcctgcgt taatcgctgc
    13801 ctgagtctgg ttttcaggcg ttttgatgta tgactggcaa taagcctcct gcattgctgt
    13861 tagtggctta aattgcgttg atttgcgttt ataggtttta ggttcagcag gcatcataac
    13921 caccgtggta atagttaccg ttgtggtaat agtaccatgc aaaataaagc cgccatagtt
    13981 ggcggcagta ttcaaaaccc atcaaattta tcatgcataa tctactcgtg acatgtcaca
    14041 ctattaattt cgtttcatgc cagcctttag tcacccagca ttgcgagtca ccattacacg
    14101 ggcatgaatt aactggaact ctctcgccgc acttaccgca acattttctg ctgatcgatt
    14161 ttatacgccc gcgcacgcgt gcatcatcct ggcggatcag taacgctata tactcaccaa
    14221 attcgtaagg cgcacgcccg gggcgacgcg tggcacagtt ccgcgctagc atttcctgct
    14281 cctgcttatc cagcaccagt tcaattttgc gctcaccggc ggcggactgc cgagcgcgct
    14341 gcgcggcttt gcgttctgcg ggggatttag ccacgaatcg cactccacgc cagattgatt
    14401 aatgactccc aggtaatata aacccggata ccagcagcca ggccgaaacc aatcaccatg
    14461 gcataaagca gagcgttgca cttgttcatc acttcacccc ctgcggcggt tccggtagcg
    14521 gcatccagtg ggttacaccg ccaattggtt catcgtcgtc gtactccaat gcggatatat
    14581 agaacccgtc acgacgagaa taagaaatcc cggacattac aatgccatcc gaaacaacaa
    14641 taatgtcacc cgtttcttcc ggcattcgct cactacagct tatccaacta tccggagtta
    14701 ccggatagtt gcgcattgcg acctttaatg cctcatagaa gcaacctttc agattgttga
    14761 actgacgccc atttagcggg acgtgttcag tcagcatgtt gtgtaatttc catgccgcgt
    14821 cgtttacttc gctggatgac aggggaggca acttgtaagt ttggcttaca ggttcggcac
    14881 catgaagcat ggcggcgcgg agtttctgta tctcccgtgc cattattgcg tcctgacgag
    14941 gagtgaacat atcacctgca ataattctgt ttagttcctc gtcagtgaac tcataatcat
    15001 caggaacatt gtgaacctca gcataaagag gctttttggt agaaatggtc atcgtaaaac
    15061 atcctccgca cttaccagtg cgtttcgcaa aagataatcc atcgccctat ctggtaattt
    15121 gcaatcaggt tttgcttttt tcagttggct gaccaattgt ttaaccagca ctgttaattc
    15181 gataacttgg tagcgcggca atggtgaatt atcagatttg ccctgactgt ctgcgctgca
    15241 tgaatgccct tccagccatg ccaatgcttg tcgcatgaaa tacgcaatat gtttgccgtg
    15301 gtaatcgtct tcatcgatgt gaaaagcgat actgcggatg tattctattg cattttcaat
    15361 ggcctccaac gctatcggcg ctggcggtgc ggtataaaac ttcgtcccca gcggaaacaa
    15421 cttcatcgct ttttctccct taatgatgcg ataagttgat tctccgccaa ggtctaccgt
    15481 tccatccata ataaggccgt atcgcttctc tgaaacttca ccaataggat ctgcttccag
    15541 tgatgccagt gcaattttaa atgcggtaag catgttgctt tgatcttcat tgagtccgaa
    15601 cggtatttca tcccgtgctg actcaatgct ggtaatcgtt ttctgtagcc attctttggt
    15661 aatagtggtc atagctattt caccttaatc tcaacatttc gcagctttag ctctactggc
    15721 aggtctgact ttccggttaa tgctaatgcg agattttctg gagtaatgag agcagttatt
    15781 gttctaccca tcgccagacg aataatcatt cgtatctcgc aatcgtcaca tgctcccggt
    15841 cgaacaattg atatttgtcc attcatctca cactcctttg atgcgaatgc cagcggcgcg
    15901 gattgcatcg atgacttcag aaactttgta tgccattacc gtttggtaat catcgtgaaa
    15961 atctgttcga tgaagcatgc tgctacgttc cgggagcagt atttcccgat cctccagttc
    16021 tgctatgcgc ttctctgctg attccagctt ctcgcgcata tcgtcaacgt actcgaccag
    16081 agaaccgcca gcaggaattt cgcactcctc gaccagttgg aagtagatat cagctgcggc
    16141 ccgtgtgttg ctatgcctag cgtcgcccat ctcaccttca cgaagagcat cgcgttcggc
    16201 ggtaagattg gctattttgc tgtctttgcc ttccagctca actcgcagcc tcccaaccgt
    16261 aagcgcaata tcctcgttct cctggtcgcg gcgtttgatg tattgctggt ttctttcccg
    16321 ttcatccagt agtgccaaga cggtagcggg attggctgcg gcgatgaatt cagcattggc
    16381 ctgctgttcc atttggaaat cttcatcgaa accgctttca ggatgcgctc cttcaattct
    16441 gcaaatagga atatatccag caacttcacg atgaattagc gcatcatcac catcaaatcg
    16501 gctctctcca tattcgagcg accactcacc acacgttgct ttttctgccg cttcacgcag
    16561 tgcctgatag tcaatcttgc tcactggttg cctcctttgc gaagctgggc agcaaagtca
    16621 cggatagtgt catcaaggtc tgggcacaac agatggtcag caaacatctc cacgccctgc
    16681 gcccgtactt cagccaggaa agcatcggtg gctggggttt tgatttcgtt aagcgcatca
    16741 ctgaatccac cacgttccat acctagctct gcttcgtaat cggcatcgaa tgcagcgtct
    16801 ttgcagaact tcttcagccc cgcattctcc gctgccagcg cattagcacg cgccagttgc
    16861 acttccagtt gcgttgccaa atcgctgatc agctttgcca cgctgcgcat atcaacggca
    16921 ccacattcag ctttcagttc cgaagccatc tcatgcccgg cggaaactaa ccctttgata
    16981 ttaccgtcca tatttaccct cgcttatcca cataacttat tgattacatt gataactaaa
    17041 aagatcgtcg attcagaact cttcgatgtt ccagccacca cctgctttct ttggtttaac
    17101 cgttaccccg atgattcgga acggatactg atctgcggcg actttggttt tcactctggc
    17161 gtcatcggtc cagaatcccc ctttcacttc gtgcagttcc atctcgccgg tggcgagcat
    17221 cacagcgaaa tcgggcgtat agaacgtgtt gtcagctaac cgcagcttga taccctcgaa
    17281 tcgataccag gcgacttccc ctgcacgttt acgctgctca aggtgctggc aatacgcaga
    17341 ttctgttttg ttcatctggc ctgttttgag tcgaccaaga gcctgtatct gttttctcat
    17401 gatttacctc taaggtaatt aaaaaccaca taagacacga aatcaataga gctaagaata
    17461 ttttgttacc tgttgggtaa ttattgaagc gtaaaaaaat gcgctatcgc gctggtatta
    17521 cttgataaat cctgccgcct ttcctcgcct gtattcctcc atcagccact gcgccggtgt
    17581 tattcccccc agggtggcgg cgttaggcat gcacccgaaa cttcgccctg gtggatggta
    17641 aacgtctctc cctgtgtccg gaggcgtact catgggttct ggctttgcct gtatgctgat
    17701 caccggatcc ggtatctgct gtccggaagc cacctttttc gcccaatcat cgagcagcct
    17761 gcgcgcgtgt ttctcaacct caatctcgct aagctggcgc tgatacattg cacggcgggt
    17821 atcacatacg acccagtaca taaccggatg ccgccacggg aatctctcgg gaccaccagg
    17881 atataaactt ttttccttgc tgtaccggtg aaactccgcc atcacatcgt caatggtgac
    17941 accaagaacc atcttgctgt ctttgcacca cttgataaat tgcccaggcg acggccagaa
    18001 cggagattca ctggcgcggg catggcgcat accagcagaa acctgttcac gggttcggat
    18061 ccccccttcg gcaaacgcag caatccactg ctgttttgca gcaacttcct gctctggcgt
    18121 cttcaggttg gttaccactg ccgccggaaa cagttgtttc aactgtttga aaagggcatc
    18181 aacaagcctc tctgctgaca tgttcaccac gttgtcattg ttggtgtact gatgctcata
    18241 acctgacatg cgagaaaggg cttctccgtc acggttttgt atcgcggtaa aaacgttgtt
    18301 cacaagaaat cctcccatgc ttcagggctg ttccagtgcg gaacgttgtt atcaggtaat
    18361 gttgattgct tctgtctgct aatctgcagc ctccttgcca gcttctgctc ccactgtgcc
    18421 tgatggtatg ccttaccctc agccatccag taaattctga actctgcaag ttcctgtgcc
    18481 gttggcagac tatccaggta gattccctgc aatgagcttt tccgaagaaa gtcatctgat
    18541 ggctgccatt gttcatgcat gacaaatttg cctaattgcc ctggcccacc aggaggaaca
    18601 aagttattca tcacggcgtt gtttgcgccg gggtcatgat gcacagaatc cccgtttttt
    18661 gtcctgctct ccctctcttg gttaaatgac tggttatatg actggttctg gatcccgttt
    18721 ttgggatcat tcaacatccc gtttttggga tcattcaaca tcccgttttt gggtatattc
    18781 ccgttttcgg taacattacc gttttcgggt tcattgcccc cctcccggtt gcctttaatg
    18841 ttcccgtttt tggttatatt aagagagaaa acccgcactc ttttcgtcgc tccctttcta
    18901 tctccgatat ctgaaataag ccccattttc atgagcgata taagcccggc ctgcacggtt
    18961 tttttattca ggcaagtgtc tttaacgagg cgttctatgc tggggtagca gaggttatat
    19021 tcatcggctc tgtcagccat cgagagcagt atgagcttta atgatgagct acctggatct
    19081 gtctcccagg cccaatctgt tgcatgtctg ctcatgatta atctccgcta tcagcttgag
    19141 tgttgtgggg aggaattaat catgatctgc ttaatctctg ccctgatgcg acggtttgat
    19201 tccatggtgc actcaacaca gtgtccgttg taaacccagc gttcactgtc atgtccgtgc
    19261 ttacatggtt ttccggtgta atagcgttta agtccgcgct ttgcggcatc aatacgtgta
    19321 atgatttcca tggtaagccc tgttattagt attgggatta cggttatttt gtgctgacac
    19381 aaaaaaaaga tcaaccatat ttggtttttt attacctttg aggtacgaat agatatgaaa
    19441 agaccgccgg atggcggtct acagagggtt gtggctggat atcatgagta gaagaagtat
    19501 gccaattctg cttttgagcg cagccattgt cttgttttac aggctttaaa aagcccattc
    19561 atcaatacct tacctggcat tttgcgctta cctgttaagt gagtctggat atagtgactc
    19621 gtcgttccgg cttcctgtgc gaaggcttca cgctcatccg gagtaagtgc aagccagtgc
    19681 tttttgaaat cgaaatgtcc gttatcgctc atagctattg cctgatattt atttcagata
    19741 ataaatattc acccataagg taacaaaaat caaggatagt tacctgtggg gtgcatttac
    19801 ctgttgggta atattgcttt aaattgaatc atctactgat tcatatatga ggcgattttc
    19861 cagaaaatga aaagtatcca ggacgtccgc aggcaaaatc tcaacgactt gatcgaccgt
    19921 gaattcaatg gtgttcagac gcggatggca gaaaaacttg gaactcaggc aaatctggta
    19981 aaccgctggg ctcttggcaa gaaggttatc ggcgaccagg ttgcgcgaaa aattgaagct
    20041 gccgccaata aaccccgtaa ctggcttgat atcgatcgct cgctttctca ggaaggtttt
    20101 cagcctgtcg gcccaagcga cattggtcag ctggcggctc acaacctgga acgctggatg
    20161 agcgaaagcc gcgacctttc aacgcaggga aaacttcacc gcgcatccgg cgtcgcccag
    20221 gtgacaatca gtcgcctgtt aaacaatgag gtcagcgttt ccatttccac cctggagaat
    20281 gttgcatccg cattcgggcg tcacggatat gaactactga ttcacccgca cgaccctgcg
    20341 actatcaact atgaccgctc acgctacgca ttgttacccg aaaccgagaa agcaaagatc
    20401 gaaagttaca ttgaatttgt catcaaccag aacgaaaaaa gcaaacaata aaactatatt
    20461 tttcagtaag taagccgcct catggcggct tttttattgc cagatagatt accttacgg
//
LOCUS       DAZFAH010000070        20443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFAH010000070 DAZFAH010000000
VERSION     DAZFAH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.070"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            873..1697
                     /gene="dinD"
                     /locus_tag="ACHJWB_002413"
     CDS             873..1697
                     /gene="dinD"
                     /locus_tag="ACHJWB_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4314035.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYLAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            1989..2606
                     /gene="yicG"
                     /locus_tag="ACHJWB_002414"
     CDS             1989..2606
                     /gene="yicG"
                     /locus_tag="ACHJWB_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4314036.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(2603..4285)
                     /gene="ligB"
                     /locus_tag="ACHJWB_002415"
     CDS             complement(2603..4285)
                     /gene="ligB"
                     /locus_tag="ACHJWB_002415"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4314037.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTEKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            4543..5166
                     /gene="gmk"
                     /locus_tag="ACHJWB_002416"
     CDS             4543..5166
                     /gene="gmk"
                     /locus_tag="ACHJWB_002416"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4314038.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            5221..5496
                     /gene="rpoZ"
                     /locus_tag="ACHJWB_002417"
     CDS             5221..5496
                     /gene="rpoZ"
                     /locus_tag="ACHJWB_002417"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4314039.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            5515..7623
                     /gene="spoT"
                     /locus_tag="ACHJWB_002418"
     CDS             5515..7623
                     /gene="spoT"
                     /locus_tag="ACHJWB_002418"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4314040.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            7630..8319
                     /gene="trmH"
                     /locus_tag="ACHJWB_002419"
     CDS             7630..8319
                     /gene="trmH"
                     /locus_tag="ACHJWB_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4314041.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            8325..10406
                     /gene="recG"
                     /locus_tag="ACHJWB_002420"
     CDS             8325..10406
                     /gene="recG"
                     /locus_tag="ACHJWB_002420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4314042.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(10391..11257)
                     /locus_tag="ACHJWB_002421"
     CDS             complement(10391..11257)
                     /locus_tag="ACHJWB_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314043.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11260..12465)
                     /gene="gltS"
                     /locus_tag="ACHJWB_002422"
     CDS             complement(11260..12465)
                     /gene="gltS"
                     /locus_tag="ACHJWB_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4314044.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            12745..14136
                     /gene="xanP"
                     /locus_tag="ACHJWB_002423"
     CDS             12745..14136
                     /gene="xanP"
                     /locus_tag="ACHJWB_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4314045.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14257..15966
                     /gene="yicH"
                     /locus_tag="ACHJWB_002424"
     CDS             14257..15966
                     /gene="yicH"
                     /locus_tag="ACHJWB_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4314046.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGVDIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDIRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(16018..18336)
                     /gene="yicI"
                     /locus_tag="ACHJWB_002425"
     CDS             complement(16018..18336)
                     /gene="yicI"
                     /locus_tag="ACHJWB_002425"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4314047.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCLSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACTWYTDKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWS
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     EVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEDTAFHLFNLQDGHETVCEVPAADGSVIFTLKAARTGNAITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEHGLVVTPQGNALTITL"
     gene            complement(18346..19728)
                     /gene="yicJ"
                     /locus_tag="ACHJWB_002426"
     CDS             complement(18346..19728)
                     /gene="yicJ"
                     /locus_tag="ACHJWB_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4314048.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSFTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            20021..20115
                     /locus_tag="ACHJWB_002427"
     tRNA            20021..20115
                     /locus_tag="ACHJWB_002427"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20055..20057,aa:Sec,seq:tca)
     gene            20194..20355
                     /gene="rhuM"
                     /locus_tag="ACHJWB_002428"
                     /pseudo
     CDS             20194..20355
                     /gene="rhuM"
                     /locus_tag="ACHJWB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         5114 a         5505 c         5112 g         4712 t
ORIGIN      
        1 aagagcacag gaacgacctc gctaatcagt taccagcgct cacgctcaaa tatccacagg
       61 aacggggcta aatcgcattc tggcgcaatg ttcactatcg gggcaagcat tcaccggaaa
      121 acgtcataca ggcttacagg cggcttaaaa ttctatgggc ggataacagg gcgggatcgg
      181 gaggcttacg aaaatttcgt aaccccccta cacaaaaagt gcaggttact acccgaaaag
      241 tgtagtgcca ttgctggggg ctaaaaaaaa ccgcagtgtg ataacactac ggcacgccac
      301 caaagaaaca taacaaggaa tcagcaatga gtaatacctg taagccttcg gggtgattgt
      361 aacacatgag caatctttta ctggaagctg tttttcactg gatcacgtat cgttatgggg
      421 attcccatat cggagagcat ccaccagctt cataacggat aaccgaaaaa tgataacggt
      481 taacctggaa aaaattccgg tgaatatttg ccctgacagt tcccatatcg gcggcacacc
      541 tgaaaaaacc gtgtacacaa ggatgtacac aataaaaatc gttattacag aaatcccagt
      601 aatgacaagc ctttgatgca gatactcaat gagcagattc aacattgaat aactcgtaac
      661 gccaattctt acttttcagc cttcacaaat gccgcccact caaacagagc ggcatttttc
      721 ttccccgcaa cttcaattct gttttgcgtg cctgctccag attttgcgat gtttttttgc
      781 ccagcacact gagaacgtga gatactcaca actgtatata aatacagtta cagatttact
      841 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
      901 aaatacggca ttatggcact gaagggcaag aattttggtc agctagagaa ttggcaccgt
      961 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     1021 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     1081 taggttcagg ggctcaacga gaacttgaag atgttcatct ctcccgctat gcctgttacc
     1141 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttatcttg
     1201 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     1261 acgaaaagcg tctttttctg agaaatgaat tgaaagaaca taacaaacaa ttggttgagg
     1321 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     1381 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     1441 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     1501 ctacccaaac agaagaaaaa ctcaagcggg atggcgtgaa ttcaaaacag caagctaata
     1561 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     1621 tgcctgagga gttgccgacc ccgcaggtca gcatcaagca gttggaaaac agtgtaaaaa
     1681 ttacagagaa gaaatagcag aaactaaaaa ctctttttgt tgattgagat acccgatgcg
     1741 taagccaaag tccaggtgcg gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     1801 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     1861 tctacgctac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     1921 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     1981 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     2041 gggctctggc ggccgggcga cgccgcatgg atacatttgg cgtaattatt attgcgaccg
     2101 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     2161 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     2221 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     2281 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     2341 ccgttgtcgc ggcggtaacc accggtgtat ttggcggcgt tttgcgcgat atgttctgta
     2401 aacgcattcc gctggtcttt caaaaagagt tgtatgccgg tgtctcattt gcctccgccg
     2461 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     2521 tggtattcgg cttttttgct cgtttactgg cgctacgtct taagctggga ttaccggtct
     2581 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     2641 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     2701 cagatccagt ccccggcagc tgctgccaga actgctccgt gctaaataaa agttgcgacc
     2761 aggaccgttc atcactggca ttaagcgccg cccgggttag cggtattccc attgccatca
     2821 cccagcgagt aaaaggctgg ttacgagcca gattaaactg atgccataac tgcgcacttt
     2881 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     2941 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca accggcctca ccaatgccat
     3001 ccagccccag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     3061 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     3121 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     3181 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     3241 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     3301 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     3361 ctacaggttg atatttccag gccaccagcc actctgcctg gcccggtagc caatggcggg
     3421 attctggctc tttcgccgct cgtacaacta cgccatcggt gacgaagggt aatttcgctt
     3481 tccaccactc attgcgtacg cgcgcaacct catcagcatt tttcaccgca cgggtatacc
     3541 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     3601 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     3661 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     3721 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     3781 taacggtttg cggcacagcg gaaattaagc taactttctc cgtccagtcc tcgcctttca
     3841 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     3901 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     3961 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     4021 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     4081 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttt ccttccttcc
     4141 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     4201 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     4261 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     4321 aacgtggtga aggcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgagaa
     4381 tcgtctttat tgcagtgaat gacaggcaaa tgcggaaaca gctacgtaaa acgcaacaac
     4441 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     4501 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     4561 tatattgttt ctgcccccag tggcgcgggt aaatccagcc tgattcaggc tttattaaaa
     4621 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     4681 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     4741 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     4801 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     4861 ggcgcgcagc aaattcgcca gaaaatgccg cacgcgcgga gtatctttat tttaccgccg
     4921 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     4981 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattacctg
     5041 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     5101 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     5161 gactgaacct gatttcagta ttatgcccag tcatttcttc acctgtggag ctttttaagt
     5221 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     5281 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     5341 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     5401 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     5461 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
     5521 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
     5581 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
     5641 gaaccctata tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
     5701 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatactcc cgccacctac
     5761 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtgtcgaaa
     5821 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
     5881 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
     5941 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
     6001 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
     6061 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
     6121 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
     6181 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
     6241 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
     6301 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
     6361 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
     6421 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
     6481 cgtaccgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
     6541 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
     6601 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
     6661 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
     6721 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
     6781 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttactag cggtcagacc
     6841 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
     6901 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
     6961 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
     7021 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
     7081 ctgctggcag aaattggtct tggtaacgca atgagcgtgg tggtcgcgaa aaatctacaa
     7141 catggggacg cctccattcc accggcaacc caaagtcacg gacatctgcc aattaaaggt
     7201 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
     7261 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaacatc
     7321 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
     7381 caggaattca tcaccgaaat caaggtggag atgttcaatc atcagggtgc gctggcaaac
     7441 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
     7501 gatggtcgcg tctacagcgc ctttattcgt ctgaccgctc gtgaccgtgt gcatctggcg
     7561 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
     7621 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
     7681 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
     7741 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
     7801 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
     7861 accattggcg atgccgtcgc tcatctcaaa ggccagggca tgcagattct ggcaacccat
     7921 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
     7981 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
     8041 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
     8101 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
     8161 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
     8221 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
     8281 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
     8341 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
     8401 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
     8461 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
     8521 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
     8581 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
     8641 gcctggcggc gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
     8701 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
     8761 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
     8821 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
     8881 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
     8941 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
     9001 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
     9061 ttcatgccca gccgctgagc gccaatgaca cgctgaaaaa taaactcctc gccgccttac
     9121 cgttcaagca aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
     9181 tggatgtgcc gatgatgcgt ctggtgcagg gcgatgtagg ttccggtaaa acgctggtcg
     9241 ccgccctcgc cgcgttgcgt gcgattgccc acggcaaaca ggtagcattg atggcaccaa
     9301 ccgaattact cgccgagcag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
     9361 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
     9421 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
     9481 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
     9541 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
     9601 tgaccgccac gccgatcccc cgcacgctgg caatgactgc gtatgccgat ctcgatacct
     9661 cggtgataga tgagctgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
     9721 cccgccgtac cgacatcatt gaccgcgtgc gccacgcctg cataactgaa ggtcgtcagg
     9781 cgtactgggt ttgtacgttg attgaagagt cggaattgct ggaagcgcag gcggcggaag
     9841 ctacctggga agagttgaaa ctggcgctac cagagttgaa cgttggcctg gtacacgggc
     9901 ggatgaaacc tgccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacacc
     9961 tgctggttgc cacaaccgtt attgaagtcg gcgttgatgt gcctaacgcc agtctgatga
    10021 ttatcgaaaa cccggagcgt ctgggtctgg cgcagttaca ccagcttcgc gggcgtgtag
    10081 gtcgtggtgc ggtggcttct cactgcgtgc tgctctacaa aacgccgctc tccaaaaccg
    10141 cgcaaattcg gctgcaagtg ctgcgcgaca gcaacgacgg ttttgtgatt gcacagaaag
    10201 acttagagat tcgcggaccg ggtgaactgt taggtacgcg tcagacgggt aatgctgagt
    10261 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcgc
    10321 gacatattca cgaacgttac ccacaacaag caaaagccct gatagaacgc tggatgccgg
    10381 agacggaacg ttactccaac gcatgaccag actcatcaca agacaagtca cgcagtaaat
    10441 cttcaatctg gatagtgagg atatccattg cctcatacgc atcctgtgca attcgcgccg
    10501 ccattgcgcc ttccggttca ccacgcagaa tttcagccgc tgcctgtacg ccagccacaa
    10561 tcatttcagg tgttaacatt tggcgaagaa aagcgcgatt atcagaggac cagccatctt
    10621 tatggcctaa tggatgcccc cagcctatcc attcttgcga ccataacgga ttggcttcta
    10681 tgggataaat tagatgcgcg gcatactgaa tagcacacac cgcttttcct ggcgtatcca
    10741 gcgcccacca gcgcgaccag agagaacgaa ttaacggcgc ggccccaccc agagtattga
    10801 acaccgccag ccagcaaaga acgttattga aaccacgctc gttatccata cgctgcaacg
    10861 cggtgtccag taaaaattgc cgtacctgct ggcgttggga agcattcatc atctcctgcc
    10921 agagataggg ccgacacaat gccttcatta aggattcttc gaagtgatca ccataaccgc
    10981 cagctaaaac ggtttcatgc cattgcgcca ggcagagcgg aaacagatag cgaaacaact
    11041 ctatttgcat cggttcgtta taaacgagat tgagtacata gtaagcactc aaatcccatt
    11101 cgctgatctg ctgccactct ttagccgcca gcgccttgag ttcggcatct gcgtaatcaa
    11161 attccgtttc ccatacacag agcactggcg gctcatcacc aaaaatatgg cgacagtgtt
    11221 cacgtagcgt ttccggttcc ttccgacgct ttttcatttt taaccggcaa aaatcggcaa
    11281 cattaaatac agcttaatca ccagcgcatt gacgatatcg ataaagaacg caccgaccat
    11341 cggcaccacc aaaaacgcca tgtgcgacgg gccaaagcgt tcagtgatcg cctgcatgtt
    11401 ggcgattgcc gttggcgttg caccgaggcc aaaaccacag tgacccgcag ccagcactgc
    11461 cgcatcgtag tttttgccca tcatgcgcca ggtaacgaag atggcataca acgccatgaa
    11521 gatggtctgt accaccagaa tcgccagcat cggcagcgcc agcgaagcca gctcccacag
    11581 tttcagcccc atcagcgcca tcgccaggaa caacgacagg cttacgttac ccagcacgga
    11641 taccgcacgc tcaaagacgc ggtaaaagcc catcattgac agaccgttgc tcagaatcac
    11701 gccaacaaac agtacacaga cgaaggtcgg cagttcaaaa gcagtgccag ccaaaagttg
    11761 cgcaacaatt ttccccaccg tcaggcagat agcaatcagc gcgatagttt caatcagcac
    11821 cagcgaggtg atcatgcggc ccacatccgg cttttcaaac gccgtcggga cttcctggtc
    11881 atccggaata ccgttcggcg tggtggagtg tttcaccaga tagcgcgcca ccggaccgcc
    11941 aatcaagccg cccagcacca gaccaaacgt cgcacaggcc atcgccactt ccgtcgcatt
    12001 ggtgaagcca tagcgttcaa tgaacaattt actccacgca gcgcccgtac cgtgaccgcc
    12061 ggaaagcgta atagaaccgg ccaacagccc catcagcgga tcaagcccta acaggctagc
    12121 cataccaatg ccaatggcat tttgcatcac caacagacca acaaccacaa tcaagaagat
    12181 gccaaccaca cgcccaccgg cacgcaaact ggcaatgttg gcgttcaggc caatggtggc
    12241 gaagaatgcc agcattaacg gatcgcgcag ggacatatca aagttgactt cccagcccat
    12301 gctttttttc agtaccagta gcgccagcgc caccaacaaa ccacccgcaa caggttccgg
    12361 tatggtgtat ttcttcaaaa aggagacgga atggaccaac ttacgcccga gcagcaacgt
    12421 cagcgttgcg gcaacaagcg ttgctaaagt atcgagatga aacatagtta ctcctttgta
    12481 tccgcatcac tctttcatac tcgtcatact tcatcgcgca gtggcaactt catgaactat
    12541 gacgggtatc gagttatgct ttcctgggtc aaattctcag ctgttaatga acaaatggca
    12601 tggattttaa gcagaaaaat accaaaagtt atataaaaaa gtgcatttat ctcatttaaa
    12661 tatttagatg aggatgtggc aaacgtttgc ttttctcttt cggtcagcta aaatgcccgc
    12721 tttgctacca cgggattgtt ttcgatgtct gtttccaccc tcgagtcaga aaatgcgcaa
    12781 ccggttgcgc agactcaaaa cagcgaactg atttaccgtc ttgaagatcg tccgccgctt
    12841 cctcaaaccc tgtttgccgc ctgtcagcat ttgctggcga tgttcgttgc ggtgatcacg
    12901 ccagcgctat taatctgcca ggcgctgggt ttaccggcac aagacacgca acacattatt
    12961 agtatgtcgc tgtttgcctc cggtgtggca tcgattattc aaattaaggc ctggggtccg
    13021 gttggctccg ggctgttgtc tattcagggc accagcttca actttgttgc cccgctgatt
    13081 atgggcggta ccgcgctgaa aaccggtggt gctgatgttc ctaccatgat ggcggctttg
    13141 ttcggcacgt tgatgctggc aagttgcacc gagatggtga tctcccgcgt tctgcatctg
    13201 gcgcgccgca ttattacgcc gctggtttct ggcgttgtgg tgatgattat cggcctgtcg
    13261 ctaattcagg tggggctgac ctccattggc ggcggttacg cagccatgag cgataacacc
    13321 ttcggcgcac cgaaaaatct gctgctggca ggcgtggtct tagccttaat tatcctgctt
    13381 aaccgtcaac gtaaccctta cttacgcgtg gcctcactgg tgattgcgat ggcggccgga
    13441 tatgcgctgg cgtggtttat gggcatgttg ccagaaagca acgaaccgat gacgcaagaa
    13501 ctgattatgg tgccaacgcc gctctattac ggtcttggca ttgaatggag tctgctgctg
    13561 ccgctgatgc tggtctttat gatcacttcg ctggaaacca ttggcgatat cacggcgacc
    13621 tctgacgttt ccgaacagcc agtgtccggt ccgctgtaca tgaaacgcct gaaaggcggc
    13681 gtgctggcaa acggcctgaa ctcgtttgtt tcggcggtgt ttaacacctt ccctaactcc
    13741 tgcttcgggc agaacaacgg agtgatccag ttaaccggtg ttgccagccg ctatgtcggt
    13801 tttgtcgtcg cgctgatgtt gatcgtgctg ggtctgttcc cggcagtgag cggttttgta
    13861 caacacattc ctgaaccggt tctgggcggc gcaacgcttg taatgtttgg caccatcgcc
    13921 gcctccggtg tgcgtatcgt ttctcgtgag ccgctgaacc gtcgggcgat tctgattatc
    13981 gcgctgtcgc tggcggttgg tctgggcgtg tctcagcagc cgctgatttt gcagtttgcc
    14041 cctgaatggc tgaaaaacct gctctcctcc gggatcgccg cgggcggtat tactgccatc
    14101 gtgctgaatc tgattttccc accagaaaaa cagtaattca ctggcggcgg taagataatt
    14161 accgccgcag tctcacctct ttcaccattc ccgccttgag gattgcgcgt aaatcgtgca
    14221 taactccctt atgtgcattt cacgggatgg aagaccatga aatttattgg gaagctgctt
    14281 ctctacattc tcatcgctct gttagtggtg atcgctggcc tctattttct tctgcaaacc
    14341 cgctggggag cagaacatat cagcgcatgg gtttccgaga atagcgacta tcatctggcc
    14401 ttcggggcga tggatcaccg tttttccgcg ccatctcata tcgtgctgga gaacgtcacg
    14461 tttggtcgtg atggccagcc cgcgaccctg gtggccaaaa gtgtcgacat tgcgctaagt
    14521 agtcggcaac tgaccgaacc acgccatgtc gataccatcc tgctggaaaa cgggacgctg
    14581 aatctcaccg accagaccgc gccgctaccg ttcaaagccg atcgtctgca actgcgtgat
    14641 atggcgttta atagcccgaa tagcgaatgg aaactgagcg cgcagcgggt aaatggcggc
    14701 gtagttccgt ggtcaccaaa agccggtaaa gtgctgggta cgaaggcaca gattcagttt
    14761 agtgccggat cgctttctct caatgatgtt cctgccacca atgtactgat tgaaggcagt
    14821 attgataacg atcgcgttac gctgactaac ctgggtgtcg acatcgcccg cgggacatta
    14881 accggaaacg cgcagcgtaa cgccgacggc agctggcaag tggaaaacct gcgcatggcg
    14941 gatatacgtc tacaaagcga aaaatcgcta accgacttct ttgcgccatt acgctctgtc
    15001 ccgtcgttgc agattggtcg cctggaagtg atcgatgctc gtttgcaagg tccggactgg
    15061 gcggtgaccg acctcgatct cagcttgcgc aacatgacct tcagtaaaga tgactggcag
    15121 acacaagaag gcaaactgtc gatgaacgct agcgagttca tttatggttc gctgcattta
    15181 tttgacccga ttataaacgc ggaattttcc ccgcagggcg tagcgctgcg ccagttcacc
    15241 agccgctggg aagggggtat ggtcagaacg tcagggaact ggctgcgtga cgggaaaacg
    15301 ttgatccttg atgatgcggc aattgccgga ctggaatata ccttgccgaa aaactggcaa
    15361 cagttgtgga tggaaacgac acccggttgg ttaaacagcc tgcaactgaa gagatttagc
    15421 gccagccgca atctgatcat tgatatcgac cctgacttcc cgtggcagct caccgcgctc
    15481 gatggttacg gtgccaacct gacgctggtt accgatcata aatggggcgt ctggagtggc
    15541 tcggcgaatc tgaatgccgc cgccgcgacg tttaatcgtg ttgatatccg tcgtccgtcg
    15601 ctggcgctaa ccgccaacag cagcacggtg aatatcagcg aactgagtgc atttactgaa
    15661 aaaggcattc tggaagccac cgccagtgtt tcacaaacgc cacaacgtca gacccatatc
    15721 agcctgaatg gacgcggtgt gccggtgaat attttgcaac attggggatg gcctgaatta
    15781 ccgttgactg gcgacggcaa tattcagctt accgccagtg gcgatattca ggccaatgtc
    15841 ccgctgaaac ctacggttag cgggcaattg catgccgtga acgccgcaaa gcagcaagtg
    15901 actcaaacca tgaatgcggg tgtcgtttcc agcggcgaag ttacatcgac ggagccggtg
    15961 cagtaaacat caatctcgcc gggtacgact cagacgtacc cggcattctt caatagatta
    16021 caacgtaatt gtcagcgcat tcccttgagg cgtcaccacc agcccatgct cactttcagc
    16081 ctgcgaacca ccttgcagac catttacttt cacaacattg cgcaagcaca gtgtccagtt
    16141 cttcgcctcg cctgcaccag tcacagtaat cgcgttgcca gtacgtgctg cttttaaagt
    16201 aaagatcacc gaaccgtcag cagcgggcac ttcacagacg gtttcatgcc cgtcttgcag
    16261 attaaagagg tggaatgccg tgtcttcgtg ccacgcgtaa tcgggacgtt gatcgttgtt
    16321 gcccagcgcc agtagggtgt tatcacgcac ataaacgggc agactcagga agctgtgctg
    16381 ctgtttatgc cagcgactac cgtcgagctc atcgttgtgc cacaggtgtg tccagcgacc
    16441 ttccggcaag tagaactgca cctcgcccgc ttcagtgaac accggagcaa ccatcacgtt
    16501 gtcgcctagc atgtattgac ggtcaaggta atcacaagcc ggatcgtccg ggaactccat
    16561 catcatggcc cgcatcatcg gcgtaccccg cgcattcgca cgcgcagctt cacgatacag
    16621 atacggcatc atgcggcatt tcagttgcgt gaagaagcgc accacatcac aggactcatc
    16681 atcataggcc cacggcacac gataagattt actgccatgt agacggctat ggctggagag
    16741 caaaccaaac gcgcaccagc gtttgtaaac gtgcgccgga gcggtattct caaagccgcc
    16801 gatatcgtgg ctccagaagc caaaacctga aagtccaata gacaaaccac cgcgcaggct
    16861 ttccgccatt gattcgtagt tagcgtaaca gtcaccaccc caatgtaccg ggaatttctg
    16921 cgcaccgacg gaggccgagc gggcaaacaa gacggcttct tcctcgccga cggtttcctt
    16981 gagtacgctc cacaccagtt cgttgtagat gtatgcataa tggttatgca ttttctgcgg
    17041 atcggaaccg tcaaaccact gaacatcggt tgggatacgt tcgccaaagt cggtcttaaa
    17101 gcaatcaacg cccatcgcga ccagaccttt cagtttgtcg gtgtaccatg tgcaggcatc
    17161 cggattggta aagtcataaa ttgccagacc tggctgccat ttatcccact gccacagcga
    17221 accgtccggg cgtttgagta aataaccttt ctcttgtaac tctttaaaga cgggggattt
    17281 ttgcccaata taggggttaa tccagacgca gattttcagc cctttcgctt tcaggcggcg
    17341 gatcatccct tccgggtccg ggaaagtcag cgggtcccac tcaaaatcgc accactggaa
    17401 ggctttcatc cagaaacagt caaagtggaa aacatgcagc ggtagattgc gttccgccat
    17461 accatcgata aagctgttta ccgtcgcttc gtcgtagttg gtggtaaatg aagtggttag
    17521 ccacagaccg aaggaccacg cgggcggcag cgccggacga ccagtaaagc gggtataacg
    17581 atcaagtacc gctttcggcg tcgggccgtc gataacaaag tattcgagat attcactctc
    17641 aacgctgaac tgcactttgg agactttctc cgatcccact tcaaaagaga ggcattgggg
    17701 atgattgacc agtaccccat aaccacggtt agtcatgtag aacgggatat ttttatacgc
    17761 ctgttcagta cttgtgccgc cgtcccggtt ccaggtctct accgtttgac cattgcgcac
    17821 cagggcagtg aagcgctctc ccagaccata aactgtttcg ccaacgccaa gatccagccg
    17881 ctcaaacata taatttcgtt gattattcgt gtcctgcacg tagccattat ttttcacctg
    17941 actaccggta atacgttcgc cgttgcgcag aaaatccagt gaccagaact cacctttgct
    18001 gacacgcgca cttaagttgc cacttttaaa ctcagcataa cgctctgtgt tttcgattgt
    18061 gaccttcacg tcctgcaaaa tattgagcgg ataatgagga ccgttattca gcgccccctg
    18121 aaaatgctca atccgcacac cgacaatacc ttcctgtggg gagaaaaagc gcagcgtaaa
    18181 taaaggcgtg tcaagctgcc aggtacgttc acgtacatca cggggggcag catagaccac
    18241 catttcatta ccctgctgtt caacctcgaa cacctgaagc gggtgaatca aattgaggcc
    18301 aggttgaatc aaccagtttc catcgctaat tttcattgcc gttccttagt tctgcacttc
    18361 ttgagaggtg aattgttgct ggcaacggcg tttgccctgg gccagctgtt ccataacggt
    18421 tttcagattg tgcgtcgtga atgagtagta gcgtttagcg ataatcgcgc tcagcaaata
    18481 acagatcgcc ggaacaatcg tgaatagcgc aataatgatg ctaatcgtgg cgctgttctg
    18541 cgctttttct gccgcatcat atccgccata agccagcatc cagccgataa gagcgccgcc
    18601 gaaggccaac cccagtttga gcacaaacag cgtgccagca aaactgatcc cggtcaggcg
    18661 tttaccattg caccactcgc cgtagtcgac ggtatcggac atcattaccc actggatagg
    18721 tgtcaccagt tgatgcaaca caccaatcac gaagatgaag acaaacatag tgatgctggc
    18781 ctgcatggga acaaagaaca tcgcgaggct aatcactgcc agcagggcgt tcgtccacca
    18841 gaagatagtg actttacatt tccagtcggt cagcggtttt gccagagcgg aaccaatcag
    18901 gttacccacg caataagtgg tgagaaaagc gacaaacact tccggcgtgc ccaaaatcca
    18961 tgtgacgtaa tacatcatcg ccccaccgcg tacgcacacc gccaggatat tgaaaatggt
    19021 tagtaaaccg acaatccgcc actggtcgtt ttgccagata tcacgtaaat cttcccgcat
    19081 agacgttgtt gtaggtggtg cttcaacgcg ttctttagtg gtgaagaaac aaaatgccag
    19141 catcatgaat gccaccacgg aaaggaccgc gataccgccc tggaaaccga gtggtttatt
    19201 atcaccgcca attaaattaa ccagtggcat catcagaaca gtagaaagca tgcctcccgc
    19261 ggtcgccagc acaaaacgcc aggattgcag cgagatacgc tgagtcgggt cattggtgat
    19321 tacaccaccc aatgcgcagt aagggatatt gacgacggta taaagtaagg taagtagggt
    19381 gtaagtaatt gctgcataga tcattttgcc gttcatactg agatctggcg tgctataggc
    19441 cagtacacag acgatcccga atggcagtgc gccaaacagt acccacggac gaaatttacc
    19501 ccagcgagag cgcgttcgat cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag
    19561 tgcacgagcg accaaaaaca tggttccgac aaaaccggca ggaatgccaa aaatatcggt
    19621 ataaaagaac atcatatata acattacgtt atcgaaaata atgtggctgg cggcgtctcc
    19681 catgccataa ccaattttct ctttaacgga caacacttca ctcttcatct gcttttcctt
    19741 gatgctatag gggtgctgag accgataacg cattttgtaa accatccctg tgagagcgaa
    19801 tattccgata tctggttatt aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa
    19861 ttccgctcat atatcattgt aaaatatggg ttttatatga actataatgc tttcgtgata
    19921 atacgctgcg tgtattaggc ggaaaaaact gatctggggg atgtagaaac tcaaggaagt
    19981 agctataatg cgccccgcct ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt
    20041 gaggcggctg gacttcaaat ccagttgggg ccgccagcgg tcccgggcag gttcgactcc
    20101 tgtgatcttc cgccaaaatg cctctcctga cgttctccaa ccccttgtaa tactgtcaat
    20161 ccagatattt tctactgctc aatattcaat cggcttcagg aatacctggt ctaaggtttt
    20221 gtgatggacg atgagcggct gaagaatccg cctgtcggtt cttctgtcct cgaacgcatc
    20281 cgcgatattc acgccagtat gcgccgtgtt tatttgcagg tgatttgccc ccctgctgac
    20341 tctcagccaa ctgtatcgcc attaaggtga agatgttgtt ttactcgccc cagaacaccc
    20401 ttttttatac gactattcta tttctttaac cgctggcgaa aaa
//
LOCUS       DAZFAH010000071        20316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFAH010000071 DAZFAH010000000
VERSION     DAZFAH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.071"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(132..878)
                     /gene="cutC"
                     /locus_tag="ACHJWB_002429"
     CDS             complement(132..878)
                     /gene="cutC"
                     /locus_tag="ACHJWB_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4314049.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIIDAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLYHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(892..1458)
                     /gene="yecM"
                     /locus_tag="ACHJWB_002430"
     CDS             complement(892..1458)
                     /gene="yecM"
                     /locus_tag="ACHJWB_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4314050.1"
                     /translation="MANWQSINELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPYEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1674..3407
                     /gene="argS"
                     /locus_tag="ACHJWB_002431"
     CDS             1674..3407
                     /gene="argS"
                     /locus_tag="ACHJWB_002431"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4314051.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIRKDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3449..3493)
                     /gene="yecV"
                     /locus_tag="ACHJWB_002432"
     CDS             complement(3449..3493)
                     /gene="yecV"
                     /locus_tag="ACHJWB_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO4314052.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3584..4072
                     /gene="yecT"
                     /locus_tag="ACHJWB_002433"
     CDS             3584..4072
                     /gene="yecT"
                     /locus_tag="ACHJWB_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4314053.1"
                     /translation="MFKFLVLTLSIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4194..4586)
                     /gene="flhe"
                     /locus_tag="ACHJWB_002434"
     CDS             complement(4194..4586)
                     /gene="flhe"
                     /locus_tag="ACHJWB_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4314054.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4586..6664)
                     /gene="flhA"
                     /locus_tag="ACHJWB_002435"
     CDS             complement(4586..6664)
                     /gene="flhA"
                     /locus_tag="ACHJWB_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4314055.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLACWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6657..7805)
                     /gene="flhB"
                     /locus_tag="ACHJWB_002436"
     CDS             complement(6657..7805)
                     /gene="flhB"
                     /locus_tag="ACHJWB_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4314056.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8007..8651)
                     /gene="cheZ"
                     /locus_tag="ACHJWB_002437"
     CDS             complement(8007..8651)
                     /gene="cheZ"
                     /locus_tag="ACHJWB_002437"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4314057.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8662..9051)
                     /gene="cheY"
                     /locus_tag="ACHJWB_002438"
     CDS             complement(8662..9051)
                     /gene="cheY"
                     /locus_tag="ACHJWB_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4314058.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9066..10115)
                     /gene="cheB"
                     /locus_tag="ACHJWB_002439"
     CDS             complement(9066..10115)
                     /gene="cheB"
                     /locus_tag="ACHJWB_002439"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4314059.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10118..10978)
                     /gene="cheR"
                     /locus_tag="ACHJWB_002440"
     CDS             complement(10118..10978)
                     /gene="cheR"
                     /locus_tag="ACHJWB_002440"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4314060.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10997..12598)
                     /gene="tap"
                     /locus_tag="ACHJWB_002441"
     CDS             complement(10997..12598)
                     /gene="tap"
                     /locus_tag="ACHJWB_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4314061.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQVLRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEYEVARHESAQLQIAPVVS"
     gene            complement(12644..14305)
                     /gene="tar"
                     /locus_tag="ACHJWB_002442"
     CDS             complement(12644..14305)
                     /gene="tar"
                     /locus_tag="ACHJWB_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4314062.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKRMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQVSALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14450..14953)
                     /gene="cheW"
                     /locus_tag="ACHJWB_002443"
     CDS             complement(14450..14953)
                     /gene="cheW"
                     /locus_tag="ACHJWB_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4314063.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFINGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14974..16938)
                     /gene="cheA"
                     /locus_tag="ACHJWB_002444"
     CDS             complement(14974..16938)
                     /gene="cheA"
                     /locus_tag="ACHJWB_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4314064.1"
                     /translation="MSMDISNFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALCQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16943..17869)
                     /gene="motB"
                     /locus_tag="ACHJWB_002445"
     CDS             complement(16943..17869)
                     /gene="motB"
                     /locus_tag="ACHJWB_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4314065.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLWKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17866..18753)
                     /gene="motA"
                     /locus_tag="ACHJWB_002446"
     CDS             complement(17866..18753)
                     /gene="motA"
                     /locus_tag="ACHJWB_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4314066.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18880..19458)
                     /gene="flhC"
                     /locus_tag="ACHJWB_002447"
     CDS             complement(18880..19458)
                     /gene="flhC"
                     /locus_tag="ACHJWB_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4314067.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19461..19811)
                     /gene="flhD"
                     /locus_tag="ACHJWB_002448"
     CDS             complement(19461..19811)
                     /gene="flhD"
                     /locus_tag="ACHJWB_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4314068.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4874 a         5602 c         4946 g         4894 t
ORIGIN      
        1 cacccgctca acagatcacg gtaatcgttc gttttttata ctgttcaggg ataaaaaaag
       61 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
      121 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      181 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      241 ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct atggacttcc
      301 agcactccgg catcgaggaa gtggtacagg ttttctgcac ggactcctgc tccggccata
      361 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      421 tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgccag attattgagt
      481 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
      541 gcagcatcta ttattttttc cattcgtggc atatcgacat taccgtcaac atcgagaacg
      601 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      661 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcggataat cggatgcaca
      721 gggatcgtca cccgctggcg cacggatttc agtacaccca acgacggcgt taagcccccc
      781 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      841 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      901 ctgctcgctg gcaacaatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      961 atcggtgacc gccagcgtcg gattcggtaa tcgttcatgc tcaccttttg gcgaactggt
     1021 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1081 attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac cttcgtacgg
     1141 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1201 taccggttca tgcaatttaa acagacaaat cggtctgcca ttaatcatat tttctgacaa
     1261 aagctcgccg cactgttcaa acccgcgacg ccagcgttca gcggtgacgt tttgatggca
     1321 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac ggcgggaaag
     1381 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgta gctcgttgat
     1441 agattgccag ttcgccataa tcactcttcg tcgttcaata aaagcgttaa tttaccctgt
     1501 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     1561 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1621 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1681 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1741 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     1801 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1861 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     1921 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1981 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     2041 acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt gacgcagcag
     2101 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2161 gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa aacgccggtg
     2221 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2281 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2341 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcactt
     2401 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc
     2461 caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta gaaagcgaag
     2521 gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg atgggcgtga
     2581 tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2641 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2701 aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2761 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2821 gcgcgggcgg tacagtgaaa ctggcagacc tgctggatga agccctggag cgtgcacgcc
     2881 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2941 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     3001 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3061 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3121 cagctccggt tatcatccgt aaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3181 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3241 tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
     3301 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3361 agctgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatca atttttcgtg
     3421 agagtgaagc ctgatcaggg ttacctgatc aggctttttt attgccatct aaatgtattc
     3481 tgaaaatgga catgccaccg ttttctcact gttgggtaag aagtcagaag cgtaatatcc
     3541 agcccctggg aaacggtaac ggttcaattt aaggaatacc gcagtgttta aatttcttgt
     3601 attaacatta agcattatct cttgccaggt ttacgcagaa gatacgctta tagtaaatga
     3661 tcatgacatt tcagccatca aagattgttg gcaaaaaaat tcaggtgatg atactgacat
     3721 taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc aattaaataa
     3781 agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg taaaaaaacc
     3841 tgataccgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg actttaggga
     3901 caaagaatgt gaattaatcc tttcaaatga ggacgttcag gatttaagtg acccttattc
     3961 tgaatcagaa tggctctcat gtatgatcat acagaccaat acgcgaactc gccagttgca
     4021 gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat aattcacata
     4081 tttttggcat gttttgttgc aagctattcc tgataaatga ttgcaacaaa acatccagcc
     4141 tttttcactg agtcattaaa catactcgcg agcgcgtaat ttttatttgt ccttcagcga
     4201 taattcacaa tcacttcatt ccgttgtacc tttagcggcg gaattaaccg cccaccgcca
     4261 ggcacttccc agataaagcg caacggttct gctgccgcta taccggaaaa ggccacggtg
     4321 gttccgctct gcccatctaa ttcgacacaa cgagattgcg aacacaagcg aacccgcagt
     4381 cctgcaggtg tcgggccgat aagctgatac cgccacgcca ccagcgtcat caatcctgaa
     4441 gcaggttgtc gagtagaaag aggcgctgac gacatcgact caccgcgatg atttagcgta
     4501 attccgacac tacttgcctg ccacatcccc tccccggcgg cttgcactag cagcggaaac
     4561 aataatattg ctaataaggc tctcattatt tgccgccaat cgtcgccgtc atgcggatat
     4621 gtcggttatc agacagttcc agattcgaca ggaccactaa ctgcggcaag ctgcggcgca
     4681 ggaagcgaga caataatggt cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca
     4741 gcatctcctg acgggatagc gcttcctgag tttgcgccag taaacgatcc gccagccctg
     4801 gctccagtcc tcccccgccc tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga
     4861 ggccaataac atggacttca tctttgccag gaaaccactg ctgggtaatc gcccgtccca
     4921 acgccacgcg cacgacggcg gttaattcat gtggatcgct ttggatgggc gcatgttccg
     4981 ccagcgtttc gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt
     5041 gcagcacttt atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct
     5101 ttggcatctc ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct
     5161 ctgcggcatg ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa
     5221 ccactgtgta cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat
     5281 tcaggccaaa tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag
     5341 ggttaatcgc cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca
     5401 tcaaaatgcg atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg
     5461 gcagaaatcc catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact
     5521 caccatcctg ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag
     5581 aatcttccag ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt
     5641 ttaccggttt gggttcggca ggcgcttttt gttcgcgtcc gcgtatccaa caggccagcc
     5701 cgagcaatcc ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga
     5761 gtaaaccgag cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca
     5821 ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg
     5881 tagaaatcac cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagcgtat
     5941 aactttccgc cgcgtgtccc atgctcatgc catgttgcag cacgccgacc agcaacccgc
     6001 cgacaacgtt aatgaccatg atgaggatcc cggcgatggc atcgccgcga acaaacttac
     6061 ttgccccgtc cattgagccg taaaaatcgg cttcctgagt cacttcggag cggcgttttt
     6121 tcgcctcatc ttcaccaatc aatccggcgt taaggtcggc gtcaatcgcc atctgcttac
     6181 ccggcatacc atcgagaaca aagcgcgcac ccacttctgc gatacgcccg gcacctttgg
     6241 taatgaccat aaagttgatg atcacgagaa tgacaaacac cacgataccg atagcgaaat
     6301 tgccaccaac gaggaagtga ccgaacgctt cgaccacctt ccctgccgcc gccgcgccgg
     6361 tatgcccttc cattaaaatg atacgggttg aagccacgtt aagtgccaga cgcaacagcg
     6421 tggtaaacaa cagaatggtc ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg
     6481 ccaccagcaa caccatgatc gacaaggcaa tattgaaggt aaacaacagg tcgagtatga
     6541 atgcgggcag tggcagcacc atcatcgaca agatcaacag gatcaaaatc ggtccggcaa
     6601 ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg cagcatcgcg gccagattac
     6661 tcatgggtcg gtttctcgtt aataaaatcc agggcttccg gcaccggaag atgagtcggt
     6721 tgtacagggc gctgtccacc agccagacgc cagcgtttca gttgccagac ccaggccagc
     6781 acttccgcca ccgcggcgta cagttgaccc gggatttgtt gaccaatctc cgcatgtcga
     6841 tacagcgctc gcgccagcgg cggcgcttca agcgtcggga cgttattttc agcgccaatt
     6901 tcacgaatgc gcagcgcgac cagccctgca cctttagcga ccactttcgg tgcgctcatt
     6961 ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg ggttattgac aatgacatcc
     7021 gctttcggca catcggccat catccgacgc cgtgcggcag ctcgctgcat ctgacggatc
     7081 cgtcctttaa catgggggtc gccttcgctt tgtttgaact catcacgaat atcctgccgc
     7141 gacattcgca gctttttcag gtggctgaag atttggaaaa agacgtcaaa tcccaccatt
     7201 ggaatgacac caagcaccac cagcagtgcg catagcccta ccaaatccat cgcattaccc
     7261 atggcggtaa tcggagactc ggccattaag cgcatcatct gcggccagtg atgccagaga
     7321 aaaaaccccg tcacgctgcc aaccaggatg gttttcaaaa ttgctttaag caactccgcg
     7381 ccagtctgag ccgagaacat ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc
     7441 ggctgcaagg atttgccgct aaataccagc ccccccagca tgaccggaga aatgagcgcc
     7501 accagcacca cgccgctaat cagcggcagc agcgccagca tggcttctct gatcagcaga
     7561 ataatctgcc cgaggatcag attcgggtca ttgataatac tatgatcaaa atgcagccca
     7621 gcggagagca tgcccgacaa tcgacgggcc agcgacacac cgccaaacca gataacacac
     7681 acgcccacta acaaaatcag caacgaggtc agttcacggg aacgcgggat ttgcccctct
     7741 tcccgcgctt tttctagtcg gtgaggtgtg ggggcttctg ttttgtcgtc gctctcgtca
     7801 gacacgtcgc caatcctgga aagagtatta agcttaaatg atgccagagc gcaaagcgtt
     7861 caatggtttg agtaaggggc aaaacaggcg ggatttaggg cttttgctgc cacacatcaa
     7921 gcatagtgtg cgtttgtcgg atgcggcgtc atcgccttat cagaccgcct gatatgacgt
     7981 ggtcacgcca catcaggcaa tacaaatcaa aatccaagac tatccaacaa atcgtccacc
     8041 tgatcctgac tggctaccac accggctttg ctggtatcga cctgaggtcc attaagcaaa
     8101 ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa cagcaccatc
     8161 agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaatgac ctgcccggtg
     8221 agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt aaagctggta
     8281 tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg ggcgtcggca
     8341 aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt tgctgatttc
     8401 tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc
     8461 tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat ggcttccgcc
     8521 gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag catacgcgtc
     8581 aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg tttgattgat
     8641 ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt tgagtttttc
     8701 ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg cttgcgccgc
     8761 agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca atgccgacat
     8821 cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg gcatgttcca
     8881 gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt cgacgccatc
     8941 ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca ctatgcgtcg
     9001 catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca tttcacactc
     9061 ctgatttaaa tacgtatcgc ctgtccggca ctaatttttg ccaacatttg ctggcttacc
     9121 tggctaagat cgaccacttc gcagacccca cccatattga tggcctcgcg cggcatgccg
     9181 aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg acgcatcgcc
     9241 aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac cgcattacgc
     9301 cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg acggttaacc
     9361 gccgggccat cgtgaatttt gatttggtaa tttgcgccac tacgcgacag ctccatatgc
     9421 cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc ttcggcttct
     9481 ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt gaaaccaggc
     9541 ggcatatgct gggtaattaa cagtgccggg ctggaaagcg gcaacggttg cagtacgtga
     9601 cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc agaactcaac
     9661 aacggccctg ccttcagcgt tgtcggtgcc gacaatggct tatgtgctgc aaggctcgcc
     9721 tttgctgctg tacgcacctt ttcagcaatc atttcgctat acgccagcat tccttcgcga
     9781 atacccagtt gcggtttggt gacaaaatct atcgccccca gctccagcgc gcgcagcgta
     9841 acttctgacc ctttgccggt cagggaagaa accatcacaa cgggcattgg acgcaaacgc
     9901 attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc cagcgtcagc
     9961 acatcgggat tgaatttctt aatcaagtca cgcgcgacca gcggatcagg cgcggtcgcc
    10021 accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg catcagtgcc
    10081 gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt agcgcataca
    10141 ccgtctgacc acgcagcgtg aagcggcgct caaggtggct aaagttttca gagtgacccg
    10201 caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc tcctgctggg
    10261 tattttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac ggccccggca
    10321 cggtgtactg tttcgccagt agattcagcg gggcaaaatc aacatagttc gccagctcct
    10381 gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac cgttgcagtt
    10441 gctgcggcgt caggtttttc aactcttcat ggcgataaat accgcttctg gctttttcca
    10501 gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcgcg gtgcccaatg
    10561 tgtcagccag cgtcatcgca atgctgtacg gctcttcgcc ggttgaagcc gccgcgctcc
    10621 atacgcgata ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg aaatgatgag
    10681 cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc tgccactcac
    10741 cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc agtcccagcg
    10801 aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca gccagaacga
    10861 tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg gaaagcgcca
    10921 ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat gaagtcatag
    10981 cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc gactcatgtc
    11041 gtgctacttc atattcttca agggtaaata ccgccacacg cgacgaaaga tggtcggcct
    11101 gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggcg ttctgctgag
    11161 tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc tgttgttctt
    11221 ccgacgcaga ggcaatttct cccataatgt cgttcacgcg ggtcaccgaa ctgacaatat
    11281 cgatcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca cgattgactg
    11341 actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg gcaagattgc
    11401 gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga gccgcttcca
    11461 ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata acgctgataa
    11521 tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc atggtactga
    11581 cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc gacgcctgtc
    11641 gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg gcggccgttt
    11701 gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg cctgcgacaa
    11761 tctccgcgat accaatgtgc atctcctggc ttcccttacg cacatcactt accgtcccac
    11821 gcaaaacctg ctgcatggtc ttcagactgg caaaaatggc ggtgatctca ttacgaccat
    11881 ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg ctaccgataa
    11941 tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg atgtagatcg
    12001 ccgcaacaat aatcatgctg ataaacacca gtgccgaaat ctgatagtta cgctggcttt
    12061 gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca aagttaacgt
    12121 caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt tgattacttt
    12181 caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt ttctccgtct
    12241 ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa cttttaaaca
    12301 gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta atgtcatccg
    12361 ccggatagct aagtgccgtc agagtgcccg ctttgttcag cgcggtactg gcctgtaaca
    12421 ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg acctgattca
    12481 gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca atctgcaaga
    12541 tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga ttaaacatca
    12601 acgctcccca tcaggcggca atgaccgcgt tagtaaatac tcgtcaaaat gtttcccagt
    12661 ttggatcttg ttcagcaatt cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac
    12721 gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac gcggaaaccg
    12781 cttgcgttaa acgactcgcc tgttcttcca gcgcagcggc ggcggcagct gattcctgca
    12841 ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat gcgacttgat
    12901 cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg tcagtcacgc
    12961 gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc agcaccgaac
    13021 cggtatcaac gcgtgcgacg gagtcttcaa tgagggcttt gatctctttt gccgcctggg
    13081 cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca cggccctgtt
    13141 cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc tggaaggcaa
    13201 taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg atctcatgca
    13261 tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg gaggcacttt
    13321 gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc gcggtgagct
    13381 gctccatgct ggcggcagtt tcttccagcg cggatacctg ctgttcagta cgggaggaaa
    13441 gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct gaaccttcgc
    13501 ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt
    13561 cgcccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca ccggcgattt
    13621 cgcgaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga atgccgtacc
    13681 acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc cactgggcaa
    13741 atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca ctgctgaggg
    13801 cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc tgagcgaaat
    13861 aagctccagt attgccataa tctagataat caatcagttc agttaacgct gtgtaatagt
    13921 ttttatattt ttcatcaata ttacgactgg tagcgaccat ttcaggtaac ggtgccattc
    13981 ttttgaattt tttataatgc gtcgctgcct gcgccaatgt tttcctggcg ctatcgagca
    14041 attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc accgctgaac
    14101 gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc tcgccctgct
    14161 gttcccgcaa ttgattggaa accacaaagc tcttctggct atggtgaagg gaagaaaaaa
    14221 acagactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt accaacagcg
    14281 tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg atctcgttat
    14341 cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg aaataaaaaa
    14401 cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat tacgccactt
    14461 ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt tcgatgttca
    14521 ccagaatcaa catccggtcg cccagtgcgc ccagtccagt gagatattct gttgaaagcg
    14581 tcacggcaaa ttccggtgcc ggacgaattt gctccgccgt caatgaaagc acgtctgaga
    14641 cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata actaccgtgt
    14701 tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca atcggcacaa
    14761 taacgccgcg cagattcgtg acgccattga taaacgctgg cgtgttcgca atccgtgtta
    14821 cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac tcttcatcac
    14881 caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt gttacattcg
    14941 tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg ccatacgttg
    15001 ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc tgccgtcgcc
    15061 aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa ggtttttaac
    15121 cacaacctgg tgctgaccaa ttaattgatc caccagcaag gcgtagcggc gaccgccact
    15181 ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga cgttgaacac
    15241 tttccacagt tcgacgatgg gcagatattc gccccgcact tccagcaccc gctcgccgcc
    15301 ggccagtgga tgcagatcgg cttcacgggg ttgcagtgat tccatgacag cattcagcgg
    15361 cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag
    15421 cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt cgacatgacc
    15481 gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc cggagacgtc
    15541 ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcatcgcga cttcgtcgtc
    15601 gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg
    15661 gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct gatgttcggc
    15721 agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt ctggcagttc
    15781 aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat
    15841 caggctcttg tcgagctcag tggaactgcc caccagcgtc agttctacct gcttgccgag
    15901 ttttcccgcc agatcacgca ccagccgggg atagcgacta aaaacatatt ccatcggcat
    15961 catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta actgccccat
    16021 gctggttatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct gggcaagcat
    16081 ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct tttctaccgc
    16141 tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca cgcggccggt
    16201 tggcgcttgt tcggctgcca gtttaagcac tggtggggtg gatatttttg gcgagacttc
    16261 tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc
    16321 atcttcggcg atgtcgcccg gtaatattgc cgagagcgaa tccgcccctt tcaccacgtc
    16381 agttaacgtt gtcagatgtc ccagttcttc ttccagcagg tcgacttccc cggccttcag
    16441 gcgcgaaagg ataattcgtc gcggcgactg actgcgactc tgctcatctt gcggttcact
    16501 tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg cttctaatgc
    16561 caattgacac aaggcctggc agatataatc gaagctggcg gcatccggct cttgcgactg
    16621 tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca gattgataat
    16681 gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt ccatcagatg
    16741 cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga tcgagtgggc
    16801 agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca aaaccagcag
    16861 atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct gataaaaatt
    16921 gcttatatcc atgctcacgc tgtcacctcg gttcggctga tggcattgtg ggaacactga
    16981 cctgtggtgc aacctcaggt ttttccaggg cgcttactgg ctcattctgg ctttcggcgt
    17041 tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac cagcaggctg atgcgacggt
    17101 tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg ccgacgacac
    17161 gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat gcattggccc
    17221 gatcggcaga aagctcccag ttgctgtaac ctttctcacc actggcgtag gggaaatcat
    17281 cggtatgacc cgaaaggcta atacggttgg gaataccatt cagcacaggc gcgatggcgc
    17341 gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac atcgggcgat
    17401 tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt ttgagatggg
    17461 gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca ccccgcaatt
    17521 tccacaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc tgcttattca
    17581 cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt tcactattag
    17641 aaatgcgatc gccgcccgta accgcagtcg ccagtggagt ccggaagtac tccgcaatct
    17701 gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa aaggccatca
    17761 ttgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc ccgtggcttt
    17821 tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg cttcctcggt
    17881 tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt caataaacga
    17941 cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag gcggtgcgta
    18001 accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc tggtttcggc
    18061 gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca ataaaatacc
    18121 gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg caggacgatc
    18181 ggctgaacct aacgcgtgaa cgacccccat tacagccgcg acaataccaa acgccggaag
    18241 tgagtccccg accagcgcca gactgttcgc tgggacttct gcctcgcttt cgtgcgtctc
    18301 aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc tgataatcag
    18361 gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc gtgggtagct
    18421 ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa acatccccat
    18481 ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat acattgcttt
    18541 ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt taatcgcttt
    18601 gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca ccagttcagc
    18661 gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga aaactgtacc
    18721 gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact gttgaccatg
    18781 acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg caaaaaaaag cagcggtacg
    18841 tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct gtactctctg
    18901 ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac gtctttttac
    18961 tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct ggtgagcgtg
    19021 ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc cactttcaac
    19081 aaaccgcact aatgtccagg cacgggttaa tgccagcagt ggtccttctt ctgcttgtgg
    19141 gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat tacacaaacc
    19201 ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt tttgttccca
    19261 ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc ttccgcgcag
    19321 ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg
    19381 caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg cttcctgaac
    19441 aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc aggctgatta
    19501 acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg gagatcgtca
    19561 acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa
    19621 accagttgat tggtttctgc cagcttaacc atttgcggaa gagtcagtgc cgctaacgtt
    19681 gtcgccattt cttcatttat gccgagacga aacatagcgg acgctttgtc ctgaacaatc
    19741 aaacgctgtg caagtagtaa atatgacaag ttgatgtcat aaatgtgttt cagcaactcg
    19801 gaggtatgca ttattcccac ccagaataac caactttatt tttatgcggt ttcaccgcac
    19861 cccgtgatgt cgccgggaag acccggcaaa aaataattag cattagaata gttgcgataa
    19921 gctgcaataa gcagagccac ctttttggtt taataagtcc ttacaaatag aagggggtct
    19981 ttacacttat ctaagatttt tcctaaatcg acgcaactgt actcgtcact acacgcacat
    20041 actacggagg agggctgcga ttttcaataa tgtgtgatgc agatcacaca aaacactcaa
    20101 ttacttaaca taaatgtata agccatactt ttgttttggg tgtatttcaa tctgttaaaa
    20161 agtttttcgc tacgctagca agcaaaaatg aaacaggaat aatcgaaatg ggatgttgcg
    20221 cacagttaaa ataactcacc gtaaataatc atctgctata aataatcact ttcatgcaat
    20281 accagataag ctatttttaa acagacactt accgca
//
LOCUS       DAZFAH010000072        20108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFAH010000072 DAZFAH010000000
VERSION     DAZFAH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.072"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(273..1085)
                     /locus_tag="ACHJWB_002449"
     CDS             complement(273..1085)
                     /locus_tag="ACHJWB_002449"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4314069.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1347..2108
                     /gene="udp"
                     /locus_tag="ACHJWB_002450"
     CDS             1347..2108
                     /gene="udp"
                     /locus_tag="ACHJWB_002450"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4314070.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2249..3676
                     /gene="rmuC"
                     /locus_tag="ACHJWB_002451"
     CDS             2249..3676
                     /gene="rmuC"
                     /locus_tag="ACHJWB_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4314071.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3771..4526
                     /gene="ubiE"
                     /locus_tag="ACHJWB_002452"
     CDS             3771..4526
                     /gene="ubiE"
                     /locus_tag="ACHJWB_002452"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4314072.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4540..5154
                     /gene="ubiJ"
                     /locus_tag="ACHJWB_002453"
     CDS             4540..5154
                     /gene="ubiJ"
                     /locus_tag="ACHJWB_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4314073.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLENWRLNDAR"
     gene            5141..6781
                     /gene="ubiB"
                     /locus_tag="ACHJWB_002454"
     CDS             5141..6781
                     /gene="ubiB"
                     /locus_tag="ACHJWB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4314074.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6860..7129
                     /gene="tatA"
                     /locus_tag="ACHJWB_002455"
     CDS             6860..7129
                     /gene="tatA"
                     /locus_tag="ACHJWB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4314075.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7133..7648
                     /gene="tatB"
                     /locus_tag="ACHJWB_002456"
     CDS             7133..7648
                     /gene="tatB"
                     /locus_tag="ACHJWB_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4314076.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7651..8427
                     /gene="tatC"
                     /locus_tag="ACHJWB_002457"
     CDS             7651..8427
                     /gene="tatC"
                     /locus_tag="ACHJWB_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4314077.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8469..9251
                     /gene="tatD"
                     /locus_tag="ACHJWB_002458"
     CDS             8469..9251
                     /gene="tatD"
                     /locus_tag="ACHJWB_002458"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4314078.1"
                     /translation="MFDIGGNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRVAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9248..9736)
                     /gene="rfaH"
                     /locus_tag="ACHJWB_002459"
     CDS             complement(9248..9736)
                     /gene="rfaH"
                     /locus_tag="ACHJWB_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4314079.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9903..11396
                     /gene="ubiD"
                     /locus_tag="ACHJWB_002460"
     CDS             9903..11396
                     /gene="ubiD"
                     /locus_tag="ACHJWB_002460"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4314080.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDIVAHIDAIWDELAIFNNGKSA"
     gene            11442..12143
                     /gene="fre"
                     /locus_tag="ACHJWB_002461"
     CDS             11442..12143
                     /gene="fre"
                     /locus_tag="ACHJWB_002461"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4314081.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12335..13498)
                     /gene="fadA"
                     /locus_tag="ACHJWB_002462"
     CDS             complement(12335..13498)
                     /gene="fadA"
                     /locus_tag="ACHJWB_002462"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4314082.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13508..15697)
                     /gene="fadB"
                     /locus_tag="ACHJWB_002463"
     CDS             complement(13508..15697)
                     /gene="fadB"
                     /locus_tag="ACHJWB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4314083.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVNQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15887..17218
                     /gene="pepQ"
                     /locus_tag="ACHJWB_002464"
     CDS             15887..17218
                     /gene="pepQ"
                     /locus_tag="ACHJWB_002464"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4314084.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17218..17832
                     /locus_tag="ACHJWB_002465"
     CDS             17218..17832
                     /locus_tag="ACHJWB_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4314085.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17871..19322
                     /gene="trkH"
                     /locus_tag="ACHJWB_002466"
     CDS             17871..19322
                     /gene="trkH"
                     /locus_tag="ACHJWB_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4314086.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGMDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19334..19879
                     /gene="hemG"
                     /locus_tag="ACHJWB_002467"
     CDS             19334..19879
                     /gene="hemG"
                     /locus_tag="ACHJWB_002467"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4314087.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHTAFQEFVKKHATRLNSMPSVFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         4683 a         5094 c         5499 g         4832 t
ORIGIN      
        1 atctttttat caggaaggtc tgagatgatt tataattaat gtgaatgtgt tgataataat
       61 taatgtttgt ttgtgaatgg gagtagatgg taaatctcta aggtgtcttc cattgaagag
      121 actcatttat gactatgact tgccgacttt cttaaggtca aatagcggag tcgtaatctc
      181 caatcaattc atactgatta ttttttgggg gacagagatg gcagcaaaac gcactcgttc
      241 actcaccatc tcaaatatag actaagagga aactaactcc gcttcccacc atactgctta
      301 aaccattcca gcatacgctg ccagccatct ttcgcagatt cggcatgata gctcgggcga
      361 taatcagcgt tgaatgcatg cccggcgtcc gggtacacga taatctctgc tttcgcatta
      421 gcagcccgca gcgcctggcg catggtttca acgctctcct gcggaatgct gttatcctga
      481 ccaccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac aggttgtttc
      541 ggtgaattca gcgacttgtc gcccgtcagt ttgccgtacc acgccactgc ggcttttagc
      601 tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa tccggtgatc
      661 attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg atcgagatcg
      721 gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt ggggatatcg
      781 gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc cagatacccc
      841 tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc
      901 actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg gtaagcaggc
      961 atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc gtccggggtc
     1021 tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt
     1081 gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat agtcagcatg
     1141 caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga atgaagagtg
     1201 atttatgtga tttgcatcac ttttggtggg taaatttatg caacgcattt gcgtcatggt
     1261 gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc ttcttctgac
     1321 taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat ctcggcctca
     1381 ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg
     1441 aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc gaattcacca
     1501 cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc
     1561 cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc ttcctgcgta
     1621 tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg gttaccacgg
     1681 cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc ccggctgtcg
     1741 ctgatttcga atgtacgact gcgctggttg aagctgcgaa atccattggc gcgacaactc
     1801 acgttggcgt gacagcttct tctgatacct tctacccagg ccaggaacgt tacgatactt
     1861 actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag gcgatgggcg
     1921 taatgaacta tgaaatggaa tctgcaaccc tgctgaccat gtgtgcaagt cagggcctgc
     1981 gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc ccgaatgctg
     2041 agacgatgaa acaaactgaa agccaagcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc
     2101 tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt
     2161 agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt ctattttgtg
     2221 cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt acgcagttat
     2281 tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg cgcagcaaaa
     2341 agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca
     2401 aattacccaa agcgagcact ggcgtgcaga gtgcgagtta ctcaataacg aagtgcgcag
     2461 cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca cgcggatgga
     2521 agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg agcagcgcct
     2581 cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc gccgggttga
     2641 tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac aactggacgg
     2701 tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc ataccctgac
     2761 ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct
     2821 gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg tagtattgac
     2881 gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat
     2941 cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa
     3001 agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt ttaacgccga
     3061 agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat
     3121 ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc tggattacgt
     3181 gctgatgttt attcccgttg aacccgcttt ttcactggcg cttgaccgcc agccggagct
     3241 gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc tgctggtggc
     3301 gttgcgtact attgccaacc tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat
     3361 cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc
     3421 gattggtcaa agtctcgaca aagcgcagga taactaccgc caggcaatga aaaaactctc
     3481 ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag gagtagaaat
     3541 taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg aagagtatcg
     3601 acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg atgaatataa
     3661 tcagcagtcg cgctagccca ttaggggtag ttaagccggg tagaaatcta gggcatcgac
     3721 gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag atggtggata
     3781 agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa aaagcggata
     3841 tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat gatttgatgt
     3901 catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc gtacgccgtg
     3961 ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa ttctcccgcc
     4021 tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg
     4081 gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg
     4141 ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg tttggtctgc
     4201 gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg
     4261 gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc aaagcctatg
     4321 atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca
     4381 gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc ctgaaagcca
     4441 tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggca ggggttgtgg
     4501 cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa acctttagtg
     4561 acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc gctgaaaaca
     4621 gcccgctcgc gtctgctggg taaagtattg cgcgtggagg taaaaggctt ttcgacgtca
     4681 ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc aggcgatgct
     4741 gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt
     4801 accgcactga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac
     4861 ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg aactgctggc cccttatacc
     4921 ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat
     4981 cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga gtggcgtatg
     5041 gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt cgagcgtgct
     5101 gttgatgccc tgaccaaacg gctggaaaac tggaggctaa atgacgccag gtgaagtacg
     5161 gcgcctatat ttcatcattc gcactttttt aagctacgga cttgatgaac tgatccccaa
     5221 aatgcgtatc accctgccgc tacggctatg gcgatactca ttattctgga tgccaaatcg
     5281 gcataaagac aaacttttag gtgagcgact acgactggcc ctgcaagaac tggggccggt
     5341 ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat ctttttccac cgcatattgc
     5401 cgatcagctg gcgttattgc aggacaaagt tgctccgttt gatggcaagc tggcgaagca
     5461 gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg attttgaaat
     5521 caagccgctg gcttctgctt ctatcgccca ggttcatacc gcgcgattga aatcgaatgg
     5581 taaagaggtg gtgattaaag tcatccgccc ggatattttg ccggtcatta aagcggatct
     5641 gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg gtcgccgtct
     5701 gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg attgatgaac tgaatttgct
     5761 gcgggaatct gccaacgcca ttcagcttcg gcgcaatttt gaagacagcc cgatgctcta
     5821 catcccggaa gtttaccctg actattgtag tgaagggatg atggtgatgg agcgcattta
     5881 cggcattccg gtgtctgatg ttgcggcgct ggagaaaaac ggcactaaca tgaaattgct
     5941 ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct ttttccatgc
     6001 cgatatgcac cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc cgaaatatat
     6061 cggcattgat tgcgggattg ttggctcgct aaacaaagaa gataaacgct atctggcaga
     6121 aaactttatc gccttcttta atcgcgacta tcgcaaagtg gcagagctac acgtcgattc
     6181 aggctgggtg ccaccagata ccaacgttga agagttcgaa tttgccattc gtacggtctg
     6241 tgaacctatc tttgagaaac cgctggccga aatttcgttt ggacatgtac tgttaaatct
     6301 gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg caactggtgt tactccagaa
     6361 aaccctgctc tatgtcgaag gggtaggacg ccagctttat ccgcagctcg atttatggaa
     6421 aacagcgaag cctttcctgg agtcgtggat taaagatcag gtcggtattc ctgcgctggt
     6481 gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac tgcctgaatt
     6541 ggtttacgac agtttgcgcc agggcaagta tttacagcac agtgttgata agattgcccg
     6601 cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg gaattggcgc
     6661 tacgttagta ttaagtggca cattcttgtt ggtcagccga cctgagtggg ggctgatgcc
     6721 cggctggtta atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca aaacacgctg
     6781 attttttcat cgctcaaggc gggccgtgta acgtataatg cggctttgtt taatcatcat
     6841 ctaccacaga ggaacatgta tgggtggtat cagtatttgg cagttattga ttattgccgt
     6901 catcgttgta ctgctttttg gcaccaaaaa gctcggctcc atcggttccg atcttggtgc
     6961 gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag
     7021 tcaggatgct gattttactg cgaaaactat cgccgataag caggcggata cgaatcagga
     7081 acaggctaaa acagaagacg cgaagcgcca cgataaagag caggtgtaat ccgtgtttga
     7141 tatcggtttt agcgaactgc tattggtgtt catcatcggc ctcgtcgttc tggggccgca
     7201 acgactgcct gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc gttcactggc
     7261 gacaacggtg cagaacgaac tgacccagga gttaaaactc caggagtttc aggacagtct
     7321 gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc gaactgaaag cgtcgatgga
     7381 tgaactacgc caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa
     7441 ggcgagcgat gaagcgcaca ccatccataa cccggtggtg aaagacaatg aagctgcgca
     7501 tgaaggcgta acgcctgctg ctgcacaaac gcaggccagt tcgccggaac agaagccaga
     7561 aaccacgcca gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc
     7621 ttccccttcg tcgagtgata aaccgtaaac atgtctgtag aagatactca accgcttatc
     7681 acgcatctga ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt gatcgtgata
     7741 ttcctgtgtc tggtctattt cgccaatgac atctatcacc tggtatccgc gccattgatc
     7801 aagcagttgc cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc gttctttacg
     7861 ccgatcaagc tgacctttat ggtgtcgctg attctgtcag cgccggtgat tctctatcag
     7921 gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt ggtgccgctg
     7981 ctggtttcca gctctctgct tttttatatc ggcatggcat tcgcctactt tgtggtcttt
     8041 ccgctggcat ttggcttcct tgccaatacc gcgccggaag gggtgcaggt atccaccgac
     8101 atcgccagct atttaagctt cgttatggcg ctgtttatgg cgtttggtgt ctcctttgaa
     8161 gtgccggtag caattgtgct gctgtgctgg atggggatta cctcgccaga agacttacgc
     8221 aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct gacgccgccg
     8281 gatgtcttct cgcaaacgct gttggcgatc ccgatgtact gtctgtttga aatcggtgtc
     8341 ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa cgacgctgaa
     8401 gcagaaagcg aaaaaactga agaataaatt caaccgcccg tcagggcggt tgtcatatgg
     8461 agtacaggat gtttgatatc ggcggtaatt tgaccagttc gcaatttgcg aaagaccgtg
     8521 atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg gctactcatc accggcacta
     8581 acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa
     8641 cggcgggcgt acatcctcac gacagcagcc agtggcaagc tgcgactgaa gaagcgatta
     8701 ttgagctggc cgcgcagcca gaagtggtgg cgattggtga atgtggtctc gactttaacc
     8761 gcaacttttc gacgccggaa gagcaggaac gcgcttttgt tgctcagcta cgcattgccg
     8821 cagaattaaa catgccggta tttatgcact gtcgcgatgc ccacgagcgg tttatgacat
     8881 tgctggagcc gtggctggat aaactgcctg gtgcggttct tcattgcttt accggcacac
     8941 gcgaagagat gcaggcgtgc gtggcgcgtg gaatttatat cggcattacc ggttgggttt
     9001 gcgatgaacg acgcggactg gagctgcggg aacttttgcc gttgattccg gcggaaaaat
     9061 tactgatcga aactgatgcg ccgtatctgc tccctcgcga tctcacgcca aagccatcat
     9121 cccggcgcaa cgagccagcc catctgcccc atattttgca acgtgttgcg cactggcgtg
     9181 gagaagatgc cgcatggctg gctgcaacca cggatgccaa tgtcaaaaca ctgtttggga
     9241 ttgcgtttta gagtttgcgg aactcggtgt tcttcacact gtgcttaatc tctttattaa
     9301 taagattaag caatagcatg gagcgagcct caccatcggg ttcggtgaaa atggcctgaa
     9361 agccttcgaa cgcgccttcg gtaataatca ccttatcacc cggataaggg gttgccggat
     9421 cgacaatgtc tttcggttta tataccgata gctgatgaat caccgccgat gggactatcg
     9481 ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg atagtcgtgg
     9541 tatgaatcac ttctggatca aattcgacaa acagatagtt gggaaacaat ggctcactga
     9601 ctgcagtacg ttttccacgc acgatttttt ccagggtgat catcggtgcc aggcaattca
     9661 cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg cagtacagta
     9721 aataccagga ttgcataatg actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc
     9781 tttacgctaa gttaattata ttccccggtt tgcgttatac cgtcagagtt cacgctaatt
     9841 taacaaattt acagcatcgc aaagatgaac gccgtataat gggcgtagat taagaggcta
     9901 caatggacgc catgaaatat aacgatttac gcgacttcct gacactgctt gaacagcagg
     9961 gtgagctaaa acgtatcacg ctcccggtgg atccgcatct ggaaatcact gaaattgctg
    10021 accgcacttt gcgtgccggt gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa
    10081 tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg
    10141 aagatgtttc ggcgctgcgt gaagttggta aattattggc gtttctgaaa gagccggagc
    10201 cgccaaaagg tttccgcgac ctgtttgata aactgccgca gtttaagcaa gtattgaaca
    10261 tgccgacaaa gcggctgcgt ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg
    10321 tcgatctcaa tcgcattccc attatgacct gctggccgga agatgccgca ccgctgatta
    10381 cctgggggct gacagtgacg cgcggcccgc ataaagagcg gcagaatctg ggcatttatc
    10441 gccagcagct gattggtaaa aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg
    10501 cgctggatta tcaggagtgg tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg
    10561 cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac ccccgttccg gatacacttt
    10621 cagagtatgc gtttgccgga ttgctgcgcg gtaccaagac cgaagtggtg aagtgtatct
    10681 ccaatgacct tgaagtgccc gccagtgcgg agattgtgct ggaagggtat atcgaacaag
    10741 gcgaaactgc gccggaaggg ccgtatggcg accacaccgg ttactataac gaagtcgata
    10801 gtttcccggt atttaccgtg acgcatatta cccagcgtga agatgcgatt taccattcca
    10861 cctataccgg gcgtccgcca gatgagcccg cggtactggg cgtggcgttg aacgaagtgt
    10921 ttgtaccgat tctgcaaaaa cagttcccgg aaattgtcga tttttacctg ccgccggaag
    10981 gctgttctta tcgtctggcg gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc
    11041 gcgtcatgat gggcgtctgg tcgttcttac gccagtttat gtacactaaa tttgtgatcg
    11101 tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt gatttgggcg attaccaccc
    11161 gtatggaccc ggcgcgggat actgttctgg tagaaaatac gcctattgat tatctggatt
    11221 ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct ggatgccacg aataaatggc
    11281 cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat attgtcgcgc
    11341 atattgacgc catctgggat gaactggcta tttttaacaa cggtaaaagc gcctgatgcg
    11401 cgtttgtttt gccctattta tcgatccgac agagaaagcg catgacaacc ttaagctgta
    11461 aagtgacctc ggtagaagct atcacggata ccgtatatcg tgtccgcatc gtgccagacg
    11521 cggccttttc ttttcgtgct ggtcagtatt tgatggtagt gatggatgag cgcgacaaac
    11581 gtccgttctc aatggcctcg acgccggatg aaaaagggtt tatcgagctg catattggcg
    11641 cttctgaaat caacctttac gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa
    11701 tcgtggtcga tattccccac ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga
    11761 ttttgattgc gggcggcacc gggttctctt atgcccgctc gattttgctg acagcgttgg
    11821 cgcgtaaccc aaaccgtgat atcaccattt actggggcgg gcgtgaagag cagcatctgt
    11881 atgatctctg cgagcttgag gcgctttcgt taaagcaccc tggtctgcaa gtggtgccgg
    11941 tggttgaaca accggaagca ggctggcgtg ggcgtactgg taccgtgtta acggcggtat
    12001 tgcaggatca tggtacgctg gcagagcatg atatctatat tgccggacgt tttgagatgg
    12061 cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg
    12121 gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc ccctgacagg cgggaagaac
    12181 ggcaactaaa actttattca gtggcattta gatctatgtc gcatccggca attggtgcac
    12241 gacgcctgat gcgacgctgg cgcgtcttat caggcctaca ttggtgccgg atcggtagac
    12301 cggataaggc gttcacgccg catccggcaa gtggttaaac ccgctcaaac accgtcgcaa
    12361 taccctgacc cagaccgata cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca
    12421 tcagattcag cagcgtggtg ctgatacgcg caccggaaca gcccagcgga tgacccagcg
    12481 cgatcgcacc accattgagg ttgatcttct cgtcaatctg ctccattagc ccgagatctt
    12541 taatgcacgg caggatctgc gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc
    12601 tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca
    12661 taatcgatgg gtcacaacca acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac
    12721 ctaattcatg ggcgcggctt tcactcatca ccagcatggc agctgcgcca tcggaaagtg
    12781 cagaagatgt gcccgccgtt accgtaccgt ttactggatc aaacgccgga cgcagcgtgg
    12841 cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga
    12901 cgccgtcggc atcgtgacca ccagttggga tgatttcatt tttaaatgcg gccgactgcg
    12961 tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga
    13021 taccgtgcat acgcgccagc atttctgccg ttaagcccat catgcccgcc gctttggcga
    13081 cattgcggct caggccgggg tgaaaatcga cgccgtgact catcggcaca tggcccatat
    13141 gctccacgcc gccaaccaga catgcctgcg catcgccagt catgatcatt cgtgctgcgt
    13201 catgcagtgc ctgcatggat gaaccacaca agcgattgac ggtaaccgcc gggacagagt
    13261 gcggcacttc tgccagcagc gccgcgttac gggcgatatt aaaaccctgc tccagcgtct
    13321 gctgcacaca accccagtaa atatcgtcga gggccgccgc ttccagcgcc gggttacgcg
    13381 ccagcaggct acgcattaaa tgagcggaga gatcttctgc acgcacgtta cgaaaagcac
    13441 cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg
    13501 tgactcctta agccgttttc aggtcgccaa ccggacgggc tggctcaacc ggaggatagt
    13561 acggttcgtt atgacgcgct ttattacgca gaccttccgg cacttcatac agcgggccga
    13621 ggtgctgata ttgctgtgcc atatcgaggt attttgcgct accgagggtg tccagccagc
    13681 ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta gaccagcgcc atatccgctt
    13741 ccgccggagt ggcgataatg ccttcctcca gacagcgcac cacttcgttg accatcggga
    13801 tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg cttcggctgg ttcacttctg
    13861 ccagcaggtc ttcaacggcg gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt
    13921 tataacgcca gaaaccgagg ccgttcttct gaccaaagcg gttggcatca aacagcgcgt
    13981 cgatggcatc gcggtaatct ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct
    14041 gagcgtgatg cgcggtatca atgcccacga catccagcag atatgccgga cccataggcc
    14101 agccaaactg tttttccatc actttgtcga tcttgcggaa atccgcgccg tcgcgcagca
    14161 gctggctgaa accggcgaaa tacgggaaca gcacgcggtt aacaaagaag ccggggcagt
    14221 cgttaaccac aatcggcgtc ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg
    14281 tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa cggcatccgg tgtaccgggt
    14341 taaaaaagtg cattccgcag aagttttccg ggcgttccag cgcgttggcc agttcgctga
    14401 taggaatggt tgaagtgtta gacgccagca cggtatccgg gcgtactttt tgttcggttt
    14461 ctgccagtac ggcttttttc actttcgggt tttcgacaac cgcttctacc acaacatcca
    14521 cgcggtcaaa cccggcgtag tcgagcgttg ggtggattgt ggagattacg ccagccagtt
    14581 tcagaccatc gatcttgccg cgctcaagct gcttgttcag cagtttcgcg gcttcggtca
    14641 tgccgagggt taacgacttg tcgttgatat ctttcatgac aaccggcacg cctttccacg
    14701 cagactggta agcgatgccg ccgcccataa tgcctgcacc cagcaccgcg gcctgtttcg
    14761 gggtttcaac gtctttggtg agtttcttcg ctttgccttt tacatattga tcgttaagga
    14821 aaatgccgac cagtgcgcgg gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt
    14881 ccaggtttaa ggcttcttca cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg
    14941 tgatgggggc cggataatgt ttccccgctg tttgtgcgac catcccttta gcgatggtga
    15001 agctcatggt ggcttcaatc ttgctcagtt ttagtggttc cagcttcggc tgacgttttg
    15061 ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaac cgcctttgcg ccttcaacca
    15121 gtttttctgc tttgactacg ccatccacca gaccgatttt cagcgcctga tccgcgccga
    15181 catctttacc ggcggcaatg atttccagcg cactgtcagc gcccagcata cgtggcatac
    15241 gtacagaacc gccaaagccc ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca
    15301 gatccggcgt cgccagacga taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg
    15361 catagccgtt gacggcagca atggtcggca ccggcagatc ttccaggcga ttaaacacgc
    15421 tattggcaaa gtgcagccac tgacttaact gttcttcagg aacgaggaac agggacaaaa
    15481 attcggtgat atcagcaccg acgataaagg ctgctttgtt cgaacgcagc agcagccctt
    15541 ttagatctga ttgctgttcc agcacgccga tggcctcgcc gaggctggcg acggtcgcag
    15601 tgtcgagttt attaactgaa cctggggcat caaataccag ttcggcaatg ccatcttcca
    15661 gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc agtctcctga atccgcaagg
    15721 tgatctggtc gtaccagatg agtcgaagtg tgtattttgt gttaaaaata tgcaaataaa
    15781 agattaaaga aatgccaatc tgatcacgct cggcagaaat cacgctctgg atgaacgatg
    15841 tgctaagatg cggagactta aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc
    15901 ctcgctctat aaaaatcata tagctacctt acaagaacgg actcgcgatg cgctggcgcg
    15961 cttcaagctg gatgcattac ttattcactc cggcgagctg ttcaacgttt ttctcgacga
    16021 tcatccctat ccgtttaaag tgaacccgca attcaaagcg tgggtgccgg taactcaggt
    16081 gccaaactgc tggttgctgg tggatggcgt gaacaagccg aaactgtggt tctatctgcc
    16141 ggttgattac tggcacaacg tcgaaccgct gccgacctcc ttctggactg aagatgtgga
    16201 agtgatcgca ctgccgaaag ccgatggcat tggtagcctg ctgcccgctg cgcgcggcaa
    16261 tatcggttat atcggtccgg tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa
    16321 catcaacccg aaaggggtga tcgactacct gcattactat cgctccttca aaaccgagta
    16381 cgaactagcc tgtatgcgtg aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga
    16441 agaagcgttc cgttctggca tgagcgagtt cgatatcaac attgcctatc tgaccgcgac
    16501 cggtcatcgt gataccgacg taccttacag caacattgtg gcgcttaacg aacacgctgc
    16561 ggtgctgcat tacaccaaac tggaccacca ggcaccggaa gagatgcgca gcttcctgct
    16621 ggatgccggg gccgaatata acggctatgc ggctgacctg acccgtacct ggtcggcaaa
    16681 aagtgacaac gactacgcac agctggtgaa ggacgttaat gatgaacaac tggcgctgat
    16741 cgctaccatg aaagcaggcg tcagctatgt ggattaccac atccagttcc atcagcgcat
    16801 cgccaaactg ctgcgtaaac atcaaatcat caccgatatg agtgaagaag cgatggtcga
    16861 aaacgatctc accggaccgt ttatgccgca cggtatcggt catccgctgg gcctgcaggt
    16921 gcatgacgta gccggtttta tgcaggatga tagcggtaca cacctcgcgg caccggcaaa
    16981 atatccgtac ctgcgctgca cccgtattct ccagccgggt atggtgttaa ccatcgaacc
    17041 gggtatctac ttcattgaat cgctactggc accgtggcgt gaagggcagt tcagcaagca
    17101 cttcaactgg cagaaaattg aagcactgaa accgttcggc ggcattcgta tcgaagacaa
    17161 cgtggtgatc cacgaaaata acgtggaaaa catgacccgg gatctgaaac tggcgtgatg
    17221 gaaagctggt taattcctgc ggcaccggtc acggtcgttg aagagatcaa aaagagccgc
    17281 ttcataacga tgttggcgca taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg
    17341 gtgcgggcag aacaccccga tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg
    17401 gatgattctc aacagctggg tttctctgac gacggggagc cggcgggaac ggcaggtaaa
    17461 ccgatgctcg cccagctaat gggcagcggc gtcggggaaa ttaccgctgt ggtagtgcgc
    17521 tactacggcg gcatattgct tggcaccggt gggttagtga aagcgtatgg cggcggcgtg
    17581 aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc cattaaccga atatactttg
    17641 caatgtgaat atagtcagtt aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa
    17701 attatcaaca gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa
    17761 gtggctgaat tttccgcaaa gctggcggat tttagccgtg gttcattgca attgttagcg
    17821 attgaagaat aatccccact tcgttttgca gaactaagga agcggcagag atgcattttc
    17881 gcgccattac ccgaatcgtt ggactactgg tcatcttatt ttcagggacc atgattatcc
    17941 ctgggctggt agcactcatc taccgggatg gagcgggccg cgcttttacc cagacctttt
    18001 ttgtcgccct cgccattggt tctatgctgt ggtggccgaa ccgtaaagag aagggcgaac
    18061 tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg ggtagtgtcg
    18121 gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac gattaccgat gcgttttttg
    18181 aatctttctc tggcctgacc accaccgggg ccactacgct ggtggggctg gattcgctcc
    18241 ctcatgccat cctcttttat cgccagatgc tgcaatggtt tggcgggatg gggatcatcg
    18301 tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag
    18361 aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa
    18421 ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg
    18481 gaatggatgc ctttgatgcc atcggccata gctttgcgac tatcgctatt ggcggcttct
    18541 cgacacatga tgccagtatc ggttatttcg acagcccgac tattaacact atcattgcta
    18601 tcttcctgct gatctccggc tgtaactacg gtctgcactt ttcactgtta agtgggcgta
    18661 gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt tatcggcgta cagtttacgc
    18721 tggtggttat ttgtacactc gtactgtggt ttcataatgt ctacagttcg gcgctgatga
    18781 caattaacca ggcgtttttc caggtggtat cgatggcgac aaccgccggg tttacgactg
    18841 acagcattgc ccgctggccg ctctttttgc cggtactgct tttatgttca gcttttatcg
    18901 gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta
    18961 agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa cgccgtctat agcattaagc
    19021 tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt ttggggattt ttctccgcct
    19081 atgcattggt gtttattgtc agtatgctgg cgattatcgc cacgggcatg gatgactttt
    19141 ctgcctttgc ctcagttgtt gcgacattga ataacctggg gccagggctt ggcgtggttg
    19201 ctgataactt taccagtatg aacccggtgg ctaaatggat cctgattgcc aacatgctgt
    19261 ttggtcgtct cgaggtcttt acattgctgg tgctctttac cccgactttc tggcgtgaat
    19321 gatggagtaa tacgtgaaaa cattaattct tttctcaaca agggacggac aaacgcgcga
    19381 gattgcctcc tacctggctt cggaactgaa agaactgggg atccagacgg atgtcgccaa
    19441 tgtgcaccgc attgaagaac cacaatggga aaattatgac cgtgtggtca ttggtgcttc
    19501 tattcgctat ggtcactacc acactgcgtt ccaggaattt gtcaaaaaac atgcgacgcg
    19561 actgaattcg atgccgagcg tcttttattc cgtaaacctg gtggcgcgca aaccggagaa
    19621 gcgtactcca cagaccaaca gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc
    19681 cgatcgctgc gcggtcattg ccggggcgct gcgttaccca cgttatcgct ggtacgaccg
    19741 ttttatgatc aagctgatta tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt
    19801 tgtctatacc gattgggagc aggtggcgaa tttcgcccga gaaatcgccc atttaaccga
    19861 caaaccgacg ctgaaataag cataaagaat aaaaaatgcg cggtcagaaa attattttaa
    19921 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    19981 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaaagcaaaa
    20041 aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaag
    20101 cgaagcgg
//
LOCUS       DAZFAH010000073        19474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFAH010000073 DAZFAH010000000
VERSION     DAZFAH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.073"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..351
                     /gene="radC"
                     /locus_tag="ACHJWB_002468"
     CDS             <1..351
                     /gene="radC"
                     /locus_tag="ACHJWB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4314088.1"
                     /translation="AREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEV
                     HPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIV
                     GGNQVFSFAEHGLL"
     gene            414..635
                     /gene="yeeT"
                     /locus_tag="ACHJWB_002469"
     CDS             414..635
                     /gene="yeeT"
                     /locus_tag="ACHJWB_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4314089.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            709..1077
                     /gene="cbeA"
                     /locus_tag="ACHJWB_002470"
     CDS             709..1077
                     /gene="cbeA"
                     /locus_tag="ACHJWB_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4314090.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPETKK"
     gene            1167..1541
                     /gene="cbtA"
                     /locus_tag="ACHJWB_002471"
     CDS             1167..1541
                     /gene="cbtA"
                     /locus_tag="ACHJWB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4314091.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1538..1732
                     /gene="yeeW"
                     /locus_tag="ACHJWB_002472"
     CDS             1538..1732
                     /gene="yeeW"
                     /locus_tag="ACHJWB_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4314092.1"
                     /translation="MMTREADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1778..1858
                     /locus_tag="ACHJWB_002473"
                     /pseudo
     CDS             1778..1858
                     /locus_tag="ACHJWB_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2147..2293)
                     /locus_tag="ACHJWB_002474"
                     /pseudo
     CDS             complement(2147..2293)
                     /locus_tag="ACHJWB_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2347..2529
                     /locus_tag="ACHJWB_002475"
     CDS             2347..2529
                     /locus_tag="ACHJWB_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO4314093.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2630..2959)
                     /gene="tmaR"
                     /locus_tag="ACHJWB_002476"
     CDS             complement(2630..2959)
                     /gene="tmaR"
                     /locus_tag="ACHJWB_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4314094.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(3131..4189)
                     /gene="yeeA"
                     /locus_tag="ACHJWB_002477"
     CDS             complement(3131..4189)
                     /gene="yeeA"
                     /locus_tag="ACHJWB_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4314095.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(4387..4860)
                     /gene="sbmC"
                     /locus_tag="ACHJWB_002478"
     CDS             complement(4387..4860)
                     /gene="sbmC"
                     /locus_tag="ACHJWB_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4314096.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4979..6145)
                     /gene="dacD"
                     /locus_tag="ACHJWB_002479"
     CDS             complement(4979..6145)
                     /gene="dacD"
                     /locus_tag="ACHJWB_002479"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4314097.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKNLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTVQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            6354..7781
                     /gene="sbcB"
                     /locus_tag="ACHJWB_002480"
     CDS             6354..7781
                     /gene="sbcB"
                     /locus_tag="ACHJWB_002480"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4314098.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPCNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQVYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(7855..9213)
                     /gene="plaP"
                     /locus_tag="ACHJWB_002481"
     CDS             complement(7855..9213)
                     /gene="plaP"
                     /locus_tag="ACHJWB_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4314099.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(9203..9265)
                     /gene="yoeI"
                     /locus_tag="ACHJWB_002482"
     CDS             complement(9203..9265)
                     /gene="yoeI"
                     /locus_tag="ACHJWB_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4314100.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9480..10408)
                     /gene="yeeY"
                     /locus_tag="ACHJWB_002483"
                     /pseudo
     CDS             complement(9480..10408)
                     /gene="yeeY"
                     /locus_tag="ACHJWB_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(10454..11278)
                     /gene="yeeZ"
                     /locus_tag="ACHJWB_002484"
     CDS             complement(10454..11278)
                     /gene="yeeZ"
                     /locus_tag="ACHJWB_002484"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4314101.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKESTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTTPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(11361..11615)
                     /gene="yoeB"
                     /locus_tag="ACHJWB_002485"
     CDS             complement(11361..11615)
                     /gene="yoeB"
                     /locus_tag="ACHJWB_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO4314102.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(11612..11863)
                     /gene="yefM"
                     /locus_tag="ACHJWB_002486"
     CDS             complement(11612..11863)
                     /gene="yefM"
                     /locus_tag="ACHJWB_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO4314103.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            12146..12196
                     /gene="hisL"
                     /locus_tag="ACHJWB_002487"
     CDS             12146..12196
                     /gene="hisL"
                     /locus_tag="ACHJWB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4314104.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    12172..12295
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            12342..13241
                     /gene="hisG"
                     /locus_tag="ACHJWB_002488"
     CDS             12342..13241
                     /gene="hisG"
                     /locus_tag="ACHJWB_002488"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4314105.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            13247..14551
                     /gene="hisD"
                     /locus_tag="ACHJWB_002489"
     CDS             13247..14551
                     /gene="hisD"
                     /locus_tag="ACHJWB_002489"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4314106.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVN
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNVETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVAISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            14548..15618
                     /gene="hisC"
                     /locus_tag="ACHJWB_002490"
     CDS             14548..15618
                     /gene="hisC"
                     /locus_tag="ACHJWB_002490"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4314107.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            15618..16685
                     /gene="hisB"
                     /locus_tag="ACHJWB_002491"
     CDS             15618..16685
                     /gene="hisB"
                     /locus_tag="ACHJWB_002491"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4314108.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQANFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            16685..17275
                     /gene="hisH"
                     /locus_tag="ACHJWB_002492"
     CDS             16685..17275
                     /gene="hisH"
                     /locus_tag="ACHJWB_002492"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4314109.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            17275..18012
                     /gene="hisA"
                     /locus_tag="ACHJWB_002493"
     CDS             17275..18012
                     /gene="hisA"
                     /locus_tag="ACHJWB_002493"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4314110.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            17994..18770
                     /gene="hisF"
                     /locus_tag="ACHJWB_002494"
     CDS             17994..18770
                     /gene="hisF"
                     /locus_tag="ACHJWB_002494"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4314111.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFHDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            18764..19375
                     /gene="hisIE"
                     /locus_tag="ACHJWB_002495"
     CDS             18764..19375
                     /gene="hisIE"
                     /locus_tag="ACHJWB_002495"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4314112.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT         4902 a         5104 c         4928 g         4540 t
ORIGIN      
        1 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg
       61 tatctgaaca accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac
      121 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc
      181 gtggtactgg cacacaatca cccgtccggt gaagtcacac ccagtaaggc agaccggctt
      241 atcaccgaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg
      301 atagtcggtg gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccttcaca
      361 accacatcac acctgttttc acttttatct tctgtcttca gaggtatccc attatgaaaa
      421 ttatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc cgtctttttc
      481 cgttctgtac cggtaaatac cgctggcacg gcagtgctga agcgtatacc ggtcgtgaag
      541 tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg ccgtaaggac agttttggtc
      601 cgtatgtccg gctgatgagc gtcaccctga actgagtggg aattctgatg agcagaatta
      661 tcgccactac cggaccattc ttagccgatt ttctgtaagg attttatcgt gtcagacaca
      721 ctccccggga caacacttcc cgacgacaat cacgaccgcc cctggtgggg gctgccctgc
      781 accgtgacgc cctgtttcgg ggcacgtctg gtgcaggagg gtaaccggtt gcattacctt
      841 gccgaccgcg ccggtatcag aggcctgttc agcgatgcag atgcgtacca cctggaccag
      901 gcctttccgc tgctgatgaa acaactggaa ctcatgctca ccagcggtga actgaatccc
      961 cgccatcagc ataccgtcac gctgtatgca aaagggctga cctgcaaagc cgataccctc
     1021 agcagttgtg gttacgttta tctggctgtt tatccgacgc ccgaaacgaa aaagtaaatc
     1081 atcagaataa tcttctgctc cggcctggtg ttttcaccac gccacttttc catttttcat
     1141 ctctgcatat caggaaaatc ttcagtatga aaacattacc cgacacacac gtacgggagg
     1201 catcgcgctg cccgtctccc gtcaccatct ggcagacact gctgtcccgg ttgctggacc
     1261 agcattacgg ccttacgctg aatgacacac cgttcgccga tgaacgtgtg attgagcagc
     1321 atattgaagc tggcatttca ctgtgtgatg cggtgaactt tctcgttgaa aaatacgcac
     1381 tggtacgtac cgaccagccg ggattcagcg cctgtacccg ctctcagtta ataaacagta
     1441 ttgatattct ccgggctcgc agggcaaccg gcctgatgac ccgcgataac tacagaacgg
     1501 taaataacat taccctgggt aagtatccgg aggcgaaatg atgacacggg aagccgacag
     1561 cgttaacgta caggcgctgg atatggggca cattgtcgtt gacattgatg gtgttaatat
     1621 cactgaactg attaataagg ccgctgaaaa cggttattca ctccgcgtgg tggatgaccg
     1681 tgactctacc gaaacaccgg caacttatgc cagccctcac cagttgctgt aagacaatgc
     1741 agtgatgcag ggtatagtgc cattaaccac agaaacagca ctgttgtcct gtattaagca
     1801 ggcaagagaa gatgtcctta ccctgcgcca tctgcagctt ctgcaccaga accggtgaat
     1861 ctcactcaac aggcttcatt tcccctgacg ccagcctgaa tacagctggc gttttcattt
     1921 ataaacagaa aggaaaaccg ataataatgg aatggacgcc ccgaccatga agcaacggta
     1981 ggatattact gataaacctt ccagtggagg tgcgttatgg ccgttttaac gattagcatt
     2041 gacctgacaa aaaatgaatt ccaaatccac ggtctgggta ggaacagaaa aatctaagct
     2101 cagaaaacgg attaaagtaa gcgtcaacgg agcaccgtat tgacgcttat ttattggtga
     2161 gaactacgtt ccatggcagg agttcgtcaa cacggttgga gggccattcc ggcagtatgc
     2221 tcagaatatg gcgcagatac gcttccggat cgataccgtt cagacggcag gtgcctgctt
     2281 ttccggcaac aagcaggaat ctcagcgctg aagcaggaat tagaggtaaa aacgccctac
     2341 cgggccatga atcacccggt cattggagta gtaaccaaag cagatttagc cagcatggaa
     2401 caaatatctt tggtgaaaag ttggttacgg gaagctggag cgcacaacgt gttagtaacc
     2461 agtgcagtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagaaggc
     2521 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
     2581 caatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
     2641 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
     2701 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
     2761 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
     2821 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
     2881 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
     2941 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
     3001 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
     3061 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
     3121 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
     3181 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
     3241 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
     3301 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
     3361 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
     3421 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     3481 tggtctggat accttcatat atcgatttgg gaatacgggt ttctttgctg gcgggcgcaa
     3541 tcagcccacg cattttcacg gcatcggtca gcagtttttg tagatggctt tccagacgtg
     3601 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     3661 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     3721 acattgccag taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt
     3781 cacctgtcgg ggaacccaca acaattgcca gcgtcacccc aatcaataat ccttgatacg
     3841 gtttcttgcc cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca
     3901 tcagcggtaa cgaaattaac tccagttgca gagcgataag acctaaaatc gaccccaaca
     3961 ccgtaccgcc aatacgttcg aaggcacgag ggacaacgtt cccccagaac gagattggcc
     4021 ccataatcac caccatggtg accagcggcc aggtactttc cgggatagta aacaggcgga
     4081 taatgagaaa agtgagcagg aatgccagcg cgacccgagt accatgcaca atgcggtaat
     4141 ggcggtatac ccggatttca aacgggctta atgacttatc ggcacgcact cagaaactcc
     4201 gtaatgaaga tgaaaagcgc tcataacaca tctgtcggcc agaaggcgcg cctctgagtt
     4261 atccgacgga cataccggga acagtgtaat ttcccggcag gagcgaaaaa tttgaaaggc
     4321 gcatcttact cttttcgctt tcaaaaaaag agtggtcttc gcgttaacac accgccctga
     4381 gatgaattag tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc
     4441 ttccgcgcca ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact
     4501 gtcctgcaag aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac
     4561 acgagccacc gccaccgcat attgaccacc tgaaatttct gtcagaatga cgccctcact
     4621 gttttcgggg agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc
     4681 ggcgggtgtt tcatccggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt
     4741 tttgctatct acccacatca tcaactgctc aaaacctttc tttaccgtct gttcccacgg
     4801 gccaacgaga tggaaacctg caacggtacg tttctcttcc tgcttaatct cgtagttcat
     4861 gacgcctcca ttgatgctgt ttttatatac agtatagttg caaaattaaa accacaagga
     4921 atgagtgttg attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc
     4981 aggccttatg gtggaaataa tcactcaggc gagaaaacat gctgccttcc ccgacagatt
     5041 ccagggtaac cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc
     5101 ctacccgctg atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg
     5161 ctttgatatg tggaatttcg gctttcggta gcaccatcca gaactcttgt accgttccca
     5221 gggcgatatt ttctttatcg ccataccaga ttcgttccgt accgaccttt ttcccacggt
     5281 gcaaaatttg caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt
     5341 cctcacgacc ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat
     5401 ctacagccga agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt
     5461 caacattcat ggttttatcc cacaataacc cgttacggtt ttgctgggtg ataccgttcc
     5521 aggtgagatt tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc
     5581 gagaaagcac agctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca
     5641 ctgtttcaaa atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa
     5701 caaactgccg ttgcccaccg gcaatatagt cagccagtgc aacacaagcg tcatttccgg
     5761 aatccacaat taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa
     5821 acatcagtga agaaccgaca aacaccggat tatctttcgc ccacgcatcg cgccctacgg
     5881 tgacaatatc gtctggcgta atgcgatgac tatcgatagc gcgatccacg acatagcccg
     5941 tcatcagctt tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagaa
     6001 tctgaccggt ggtgtaatcc atcaataccc aggacccggc atgaatctct ggaggctgag
     6061 gtgaaaaagg aatgttttcc gccgcaaaac cagacgataa attaaaaacg aacaaagaag
     6121 cagcaataat aagacggcgt ttcaacagca aaccctcagg tgtttcaaat agctgttctt
     6181 tttacggaaa tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctactatctc
     6241 atttttatct gacatgatct gttgccactc gctgccaaat tgtcgcgcta aagctgatta
     6301 gcacggtgat atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga
     6361 atgacggtaa gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggaacgcacc
     6421 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac tgataacgaa ttcaatgtca
     6481 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag
     6541 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     6601 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     6661 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     6721 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     6781 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     6841 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     6901 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     6961 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     7021 ttccgcagat gaaacccctg gttcacgttt ccggaatgtt tggggcatgg cgcggcaata
     7081 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggtgg
     7141 atttggcagg agacatttcg ccattactgg agctggatag cgacacattg cgcgagcgtt
     7201 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     7261 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     7321 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     7381 tgcgtgaaaa agtggtggcg atattcgcgg aagccgaacc gtttacgcct tcagataacg
     7441 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     7501 tgctggaaac cgagccgtgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     7561 ttgaaaaact gttgttcaat tatcgagcgc gcaacttccc ggggacgctg gattatgccg
     7621 agcagcaacg ctggctggag caccgccgcc aggtcttcac gccagagttt ttgcaggtct
     7681 acgctgatga attgcagatg ctggcacaac agtatgccga tgacaaagag aaagtggcgc
     7741 tgttaaaagc gctttggcag tacgcggaag agatcgtctg aaacaaaaaa gccggagcga
     7801 caaactccgg ctttttgaga tttaccggat gattatgtca tccggtttgt aagattacgc
     7861 tacgtcttcg tactgcggaa ccgggttacg gaaactcttg gttacgcaag ccagataaat
     7921 caggccgata gcagcccaga tcagacccag aaccattgag ctttcttcca ggttaaccca
     7981 cagcgcacca acggtcagcg caccacacat cggcaggaac agatactgga agtgatcttt
     8041 cagcgtcttg ttacgcttct cacggatcca aaactgcgag atgaccgaca ggttaacgaa
     8101 ggtgaacgcc accaacgcac caaagttaat cagcgccgta gccattacca ggtcgaagtt
     8161 gattgccagc agggcaatcg cgccaaccag gatgatgttc atcgccggag tacgccattt
     8221 cgggtgtaca taaccgaaga agcttttcgg gaacacgccg tcacgaccca ttacgtacat
     8281 cagacgcgca acgcctgcgt gcgccgccat accggacgcc agtacggtaa tggtggagaa
     8341 gatcagcgcg ccaacctgga acgctttacc cgccacgtac agcatgattt caggctgtga
     8401 cgcatccgga tctttaaagc gagagatatc cgggaagtac aactgcagga agtaagttgc
     8461 aaagatgaag atcatgccgc cgatcagcgc ggtcaggaaa atcgcacgcg ggatcacgcg
     8521 ctctgcatct ttggtttctt ccgacaggtt gctgatgccg tcaaagccgg taaaggagaa
     8581 gcacaggatt gtcgccccgg taatcatcgg gataacatgc gcatcgccag accagaacgg
     8641 acgcgtgctc gccagcgtac cagcgccttc accttcaaat acgccataaa caaccatgcc
     8701 cagaatcacc gcgatcagca ctacctgcaa cacgacgatt acagtgttga agttcgctac
     8761 ggatttcaga ctacgcaggt taaaggcggt cataaaggcc accagcgcca ccacaaacat
     8821 ccacgatgga atggaaggca ccagagcttc aaaatagatt ttcgccagca gaatgttgat
     8881 catcggcgcg aacagatagt cgagcagaga agaccacccc accataaagc cgacagtcgg
     8941 gctaatggat ttctgggcgt aagtgtatgc agagccagca gaaggatagc ggcgaaccag
     9001 cttcccgtag ctcagagccg taaacaggat cgcaatcaat gcgaacgcat aggctgtcgg
     9061 cacatgacca tccgtgaggc ctgaaacgat accaaatgta tcaaacagcg tcatcggctg
     9121 catataggct agacccatca ttacaaccgg aactaacgta agcgttttac gcaattccac
     9181 gcgagaggtg tttggagtaa cgttatgcga catggtcatt ctcctttacg gtgataaccg
     9241 tcgcgtaagc aaaaaattgc cccatttttt tggattcctc agcgacaaca actgtcgatt
     9301 tttagtaaat atctatccgg tacgaagccc ggcctcttgg tatgaatgat tggtttgaag
     9361 caaaaaataa ccgacgctga tgaaacgtcg gtttttagtc attttttgac agcggcgcat
     9421 tgtgcctaaa tgggggggaa atgacaagag aatgagaggc ttgtcataat aatttttctt
     9481 taaatggctg attttccgtc atcagatttt gcataaacac cggcgatcgc actatttgct
     9541 aaaatttcat ctcgccacca gccgctggca agccctgcgg tctgttcgtt ccagccaatc
     9601 cagacgggtt caaagagagt ttgggcgacc actttcttct cgattaacgc gccactatcg
     9661 agaaaacgtt gcgccagata cggggtaaat agccgcagcc gaggccgcta atttgcagct
     9721 ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg ttcgtcgaga agttgtgagg
     9781 ccgtagaagc gcctgcctgc acagtatccc ccaccacaat cgcccgatat cgcttaataa
     9841 tacgacgatt taacggctct tcttccaggg ctaacggatg atgaggcgcg accgcaaaga
     9901 cttgttccag atcgcccaac cgcgagaaac caaactcact ggaagatagt ggctcatgca
     9961 ttgcgccaac aataatatcc gctcgcccct gagtcagggc atcccaggaa ccaccgagta
    10021 cgccattgat aaacttcagg cgcgtcacgc tatgatgttg ataaaaagct tcaatgagtg
    10081 gagcaagcag agaaaaagga aacgtgtcgt ccacgccaat caccagctcg ttttcccagc
    10141 cttcgtgcag tttgatcgcc tgcttttcca gttcccgcac ggtatgcaga acttcccgcc
    10201 ctttctctaa taacattttt ccggtacggg tgaatttagc gcggtgaccg ctacgatcaa
    10261 gcaattgaat attaaggtcg ctttccagct tgtgaacggt gtaactcaga gcggacggcg
    10321 tcttgtagag tttggccgac gccgccgcaa aactgccttc tttttctaag gcatcgagga
    10381 tcatcaacac atccagcagt ggtttcatac acgccccctt gcagtgcatg cggcgtcctg
    10441 ctggcgtgat cgtttactcc agcggcatta ccagcggatc gggatactgg tattcaaatc
    10501 ccagttcatt acaaatccga ctgccatcaa taatcttacc tttgccgctg tccagactat
    10561 ttctgaactg cggaggttcc agccccagta aacgggccat ctgcggatag aaaacattac
    10621 gcgcagggtg agctggcgca catatattat agatgtgtcc gcctttaggt gcctgtaaca
    10681 acagagtgat agcgccaata acatcttcta aatggactaa attaacacca tgttcaccat
    10741 caggcgtggt ttttccggca aagaagcgtc cgggatgacg ccccggtccg accagccccg
    10801 caagacgcag aatatcgacc gaagtaccgg gtaaattgtg caaccagtct tcgagttctt
    10861 ctaatactcg tccactgttg gtcaccggat tacgcggggt gctttctttc accgtgcctt
    10921 gcgcgtcgcc atagacagac gttgagctgg taaaaataat tcggggaata cgatgggcca
    10981 gcgcgctatc cactaactct tgtaccgctt gtaaatagaa ctcatcgccg gggccgctac
    11041 gacgtgccgg aagcgtaatg accagcgcat cggcatccat cagggcatcc agatcgtcag
    11101 aatcgcaaac taactcaggc tccatgcgaa gcagatagct gtcaatgcca ctcattcggg
    11161 ccgcttcgac accatcttgt gtggttttac tcccggtgac ttgccagcct cgcgctgaaa
    11221 gtgacatcgc cagcggcatg cctaaccacc ctaacccgac aattgcgacc tttttcatcc
    11281 gttatctcct gatacacctt agatctataa ggctacgcta gcgtatcaaa actgacaatt
    11341 cattctatga atgaatctgt tcaataatga taacgacatg ctgcaatgag cagtgaatca
    11401 tcagtaaccg cgtataccag acggtgctcc tctgtaatgc gtcgggacca gaaacctgac
    11461 aaattatgtt tcaggggttc tggtttcccc ttaccttcaa atggtgttct gcgggtatct
    11521 ttgataagtt cattgatctt tttaacaatt cgcttatctg tttcctgcca gtacagataa
    11581 tcatcccatg attcctcaga ccagattagt ttcactcaat aatgtccttt tccgttcctt
    11641 tgcctgattt caggctatcg attgagtcca tcaatctccg ggcgttagcg ggggaacgca
    11701 gtagataagc cgtctcttcc agcgagttgt attcttcgag tgacatcaga acacaagcct
    11761 ctccattctg acgagtaatg aggatcgggg catgatcttc aacggctttc atcattgttg
    11821 ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg catgtaaatc tcctcttttg
    11881 tacagttcat tgtacaatga tgggtgttaa ttaactattt attaattagt ttgtagatca
    11941 agacattgtc agcgagacga aaaatcagac tttgctcttt ttggtgctgt caagttagag
    12001 gacagtcctc ttagccccct ccttcccccg ctcattcatt aaacaaatcc atcgccataa
    12061 aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa agacatcagt
    12121 tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc accatcatca
    12181 ccatcatcct gactagtctt tcaggcgatg tgtgctggaa gacattcaga tcttccagtg
    12241 gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt tttattgcgc
    12301 ggttgataac ggttcagaca ggtttaaaga ggaataacaa aatgacagac aacactcgtt
    12361 tacgcatagc tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa ttgctggcgc
    12421 gctgtggcat taaaattaat cttcacaccc agcgcctgat cgcgatggca gaaaacatgc
    12481 cgattgatat tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg gacggcgtgg
    12541 tagaccttgg gattatcggc gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc
    12601 agggtgaaga tccgcgctac tttaccttgc gtcgtctgga tttcggcggc tgccgccttt
    12661 cgctggcaac gccggttgat gaagcctggg acggcccgct ctccttaaat ggtaaacgta
    12721 tcgccacctc ttatcctcac ctgctcaagc gttatctcga ccagaaaggc atctctttta
    12781 aatcctgctt actgaacggt tctgttgaag tcgccccgcg tgccggactg gcggatgcga
    12841 tttgcgatct ggtttccacc ggtgccacgc tggaagctaa cggcctgcga gaagtcgaag
    12901 ttatctaccg ctcgaaagcc tgcctgatcc agcgcgatgg cgaaatggaa gaatccaaac
    12961 agcaactgat cgacaaactg ctgacccgta ttcagggtgt gatccaggcg cgcgaatcaa
    13021 aatacatcat gatgcacgca ccgaccgaac gtctggatga agtcatcgcc ctgctgccag
    13081 gtgccgaacg cccaaccatt ctgccgctgg cgggagacca acaacgcgta gcgatgcaca
    13141 tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa actgaaagcg ctgggtgcca
    13201 gttcaatcct ggtcctgccg attgagaaga tgatggagtg atcgccatga gctttaacac
    13261 aatcattgac tggaatagct gtactgcaga gcaacaacgc cagctattaa tgcgcccggc
    13321 gatctccgct tctgaaagca ttacccgcac tgttaacgat attctcgata acgtgaatac
    13381 gcgtggcgat gaggccctgc gggaatacag cgcgaagttt gataaaacca cggttaccgc
    13441 gctgaaggtg tctgctgagg agatcgccgc cgccagcgaa cgcctgagcg atgaactaaa
    13501 acaggcgatg gcggtggcag ttaagaatgt tgaaaccttc cacactgcgc aaaaactgcc
    13561 gccggtagat gtagaaacgc agccaggcgt gcgttgccag caggtcacgc gcccggtcgc
    13621 ttcagttggg ttgtatattc ctggcggctc cgccccgctc ttctcaacgg tattaatgct
    13681 ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg ctgtgctcac cgccgccgat
    13741 tgccgatgag atcctttatg cggcgcagct gtgcggtgtg caggacgtgt ttaacgtcgg
    13801 cggcgcacag gccattgccg cactggcgtt tggtacggaa tctgtgccga aagtggacaa
    13861 aatcttcggg ccgggtaacg cctttgtcac cgaggcaaaa cgccaggtaa gccagcgtct
    13921 ggacggtgcg gcgatcgata tgcccgcagg cccgtcggaa gtgctggtga ttgctgacag
    13981 cggcgctacg ccggatttcg tggcttctga tttgctttct caggctgaac acggtccgga
    14041 ctcacaggtg attttactga cgcccgatgc tgatatggcg cgtcgcgttg ccgaggctgt
    14101 cgaacgccaa ttggcagaac tgccgcgtgc cgaaaccgcc cgtcaggcac tgagcgccag
    14161 ccgcctgatc gtgaccaacg atttagcgca gtgtgtggca atctccaacc agtacggccc
    14221 ggagcacctg atcattcaga cccgcaacgc tcgcgatctg gtcgatggca tcaccagtgc
    14281 cggttcggta tttcttggtg actggtcacc ggaatcggca ggtgattacg cctccggcac
    14341 caaccacgtg ctgccgactt acggttacac cgccacctgt tccagcctcg ggctggcaga
    14401 tttccagaag cgtatgaccg tgcaggaact gtcgaaagta ggtttctccg cgctggcttc
    14461 gaccattgaa acactggccg ccgccgagcg cctgaccgcc cacaaaaatg ccgttacttt
    14521 gcgtgttaac gcccttaagg agcaagcatg agcaccgtga ctattaccga tttagcgcgt
    14581 gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg taatggcgac
    14641 gtctggctga acgccaacga ataccccaca gccgtggagt ttcagcttac tcagcaaaca
    14701 ctcaaccgct acccagaatg ccagccgaaa gcggtgattg aaaattacgc gcaatatgca
    14761 ggcgtaaaac cggagcaggt gctggtcagc cgtggcgcgg acgaaggtat tgaactgctg
    14821 attcgcgctt tttgcgaacc gggtaaagac gccatcctct actgcccgcc aacgtacggc
    14881 atgtacagcg tcagcgccga aacgattggc gtcgagtgcc gcacagtgcc gacgctggac
    14941 aactggcaac tggacttaca gggcatttcc gacaagctgg acggcgtaaa agtggtctat
    15001 gtttgcagcc ccaacaaccc gaccgggcaa ctgatcaatc cgcaggattt tcgcactctg
    15061 ctggagctaa cccgcggaaa agccattgtg gttgccgatg aagcctatat cgagttttgc
    15121 ccgcaggcat cgctggctgg ctggctgacg gaatatccgc acctggctat tttgcgcaca
    15181 ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat ttacgctggc aaacgaagaa
    15241 gtcatcaacc tgctgatgaa agtgattgcc ccctacccgc tctcgacgcc ggttgccgac
    15301 attgcggccc aggcgttaag cccgcagggg atcgtcgcca tgcgtgagcg ggtggcgcaa
    15361 attattgcag aacgcgaata cctgattgcc gcactgaaag agatcccctg cgtggagcag
    15421 gttttcgact ctgaaaccaa ctacattctg gcgcgcttta aagcctccag tgcagtgttt
    15481 aaatctttgt gggatcaggg cattatctta cgtgatcaga ataaacaacc ctctttaagc
    15541 ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat tgacgcctta
    15601 cgtgcggagc aagtttgatg agtcagaagt atctttttat cgatcgcgat ggaaccctga
    15661 ttagcgaacc gccgagtgat tttcaggtgg accgttttga caaactcgcc tttgaaccag
    15721 gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta taaactggtg atgatcacca
    15781 accaggatgg tctggggacg caaagtttcc cgcaggcgaa tttcgatggc ccgcacaacc
    15841 tgatgatgca gatcttcacc tcgcaaggcg tgcagtttga tgaagtgctg atttgcccgc
    15901 acctgcccgc cgatgaatgc gactgccgta agccgaaagt aaaactggtg gagcgttatc
    15961 tcgctgagca agcgatggat cgcgccaaca gttatgtgat tggcgatcgc gcgaccgaca
    16021 ttcaactggc ggaaaacatg ggcattactg gtttacgcta cgaccgcgaa accctgaact
    16081 ggccaatgat tggcgagcag ctcactaaac gagaccgtta cgcccatgtg gtgcgcaaca
    16141 ccaaagagac gcaaattgac gtccaggtgt ggctggatcg tgaaggtggc agcaagatta
    16201 ataccggcgt tggcttcttt gatcacatgc tggatcagat cgccacccac ggcggtttcc
    16261 gtatggaaat caacgtcaaa ggcgacctct atatcgacga tcaccacacc gtcgaagata
    16321 ccggcctggc gctgggtgaa gcgttaaaaa ttgcccttgg cgacaaacgc ggtatttgcc
    16381 gctttggttt tgtgctaccg atggacgaat gccttgcccg ctgcgcgctg gatatctctg
    16441 gtcgcccgca cctggaatat aaagccgagt ttacctacca gcgcgtgggc gatctcagca
    16501 ccgaaatgat cgagcacttc ttccgttcgc tctcatacac catgggcgtg acgctacacc
    16561 tgaaaaccaa aggtaaaaac gatcatcacc gtgtagagag cctgttcaaa gcctttggtc
    16621 gcaccctgcg ccaggccatc cgcgtggaag gcgacaccct gccctcgtca aaaggagtgc
    16681 tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat ctgaactcgg tgaagtctgc
    16741 cattgcgcgc cacggttatg aacccaaagt cagccgtgac ccggacgtcg tgttgctggc
    16801 cgataaactg tttttacccg gcgtaggcac cgcacaagca gcgatggatc aggtgcgtga
    16861 gcgcgaactg tttgatctca tcaaagcctg tacccaaccg gtgctgggca tctgcttagg
    16921 gatgcaactg ctggggcggc gcagcgaaga gagcaacggc gtcgacttgc tgggcatcat
    16981 tgacgaagac gtgccgaaaa tgaccgactt tggtctgcca ctgccacata tgggctggaa
    17041 ccgcgtttat ccgcaggcag gcaaccgcct gtttcagggg attgaagacg gcgcgtactt
    17101 ttactttgtt cacagttacg caatgccggt caatccgtgg accatcgccc agtgtaacta
    17161 cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc tatggcgtgc agtttcaccc
    17221 ggagcgttct ggtgccgctg gcgctaagct gctgaaaaac ttcctggaga tgtaatgatt
    17281 attccggcat tagatttaat cgacggcacc gtggtgcgtc tccatcaggg cgattacggc
    17341 aaacagcgcg attacggtaa cgacccgctg ccgcgcttac aggattacgc cgcgcagggt
    17401 gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag atccggctaa acgtcaaatc
    17461 ccgctgatta aaactctggt ggcgggcgtt aacgttccgg tgcaggttgg cggcggcgtg
    17521 cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg ttgcgcgcgt agtggtcggc
    17581 tccaccgcgg tgaaatcacc agaaatggtg aaaggctggt ttgaacgttt cggtgccgat
    17641 gccttagtgc tggcgctgga tgtccgtatt gacgagcaag gcaacaagca ggtggcggtc
    17701 agcggctggc aagagaactc gggcgtttca ctggaacaac tggtggaaac ctatctgccc
    17761 gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg acggcacgct ggcaggttct
    17821 aacgtctctt tatatgaaga agtgtgcgcc agatatccgc aggtggcgtt tcagtcctcc
    17881 ggcggcattg gcgacattaa cgatgttgct gctctacgtg gtactggcgt gcgcggcgta
    17941 atagttggtc gtgcattact ggaaggtaaa ttcaccgtga aggaggccat cgcatgctgg
    18001 caaaacgcat aattccgtgt ctcgacgttc gtgatggtca ggtggtgaaa ggcgtacagt
    18061 ttcgcaacca tgaaatcatt ggcgatatcg tgccgctggc aaaacgctac gccgaggaag
    18121 gtgcagacga actggtgttc tacgatatca ccgcttccag cgatggccgt gtggtagata
    18181 aaagctgggt atctcgcgtg gcggaagtga tcgacattcc gttctgcgtg gcgggtggga
    18241 ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg cgcggataaa atttccatca
    18301 actcccctgc gctggcggat ccgacattaa tcactcgcct ggcggatcgc tttggcgtgc
    18361 agtgtattgt tgtcggtatt gatacctggt acgacgctga aaccgggaaa tatcatgtga
    18421 atcaatatac cggcgatgaa agccgcaccc gcgtcactca atgggaaacg ctcgactggg
    18481 tacaggaagt gcaaaaacgc ggtgccggag aaatcgtcct caacatgatg aatcaggacg
    18541 gcgtgcgtaa cggttacgac ctcgaacagc tgaaaaaagt gcgtgaagtt tgccacgtcc
    18601 cgctgattgc ctccggtggc gcgggcacca tggaacactt cctcgaagcc ttccacgatg
    18661 ccgacgttga cggcgcgctg gctgcttccg tattccacaa acaaataatc aatattggtg
    18721 aattaaaagc gtacctggca acacagggcg tggagatcag gatatgttaa cagaacaaca
    18781 acgtcgcgaa ctggactggg aaaaaaccga cggacttatg ccggtgattg tgcaacacgc
    18841 ggtatctggc gaagtgttaa tgctgggcta tatgaacccg gaagccttag ataaaaccat
    18901 cgaaagcggc aaagtcacct tcttctcgcg cactaaacaa cggctgtgga ccaaaggcga
    18961 aacgtcgggc aatttcctca atgtggtgag tattgcgccg gactgcgaca acgacacgtt
    19021 actggtgctg gcgaatccca tcgggccaac ctgccacaaa ggcaccagca gctgcttcgg
    19081 cgacaccgct caccagtggc tgttcctgta tcaactggaa caactgctcg ccgaacgtaa
    19141 atctgccgac ccggaaacct cctacactgc caaactgtat gccagcggca ccaagcgcat
    19201 tgcgcagaaa gtaggcgaag aaggcgtgga aaccgcactg gccgcgactg tacatgaccg
    19261 ctttgagctg accaacgagg cgtctgattt gatgtatcac ctgctggtgc tgttgcagga
    19321 tcaggatctg gatttaacga cggtgattga gaacctgcgc aaacggcatc agtgagttgc
    19381 ggggtaagcg gatgcgacaa caacatcgca tccggcaaaa aaaacgggca aggtgtcacc
    19441 accctgccct ttttctttaa aaccgaaaag atta
//
LOCUS       DAZFAH010000074        19208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFAH010000074 DAZFAH010000000
VERSION     DAZFAH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.074"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..455)
                     /locus_tag="ACHJWB_002496"
     CDS             complement(<1..455)
                     /locus_tag="ACHJWB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4314113.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEV"
     gene            complement(580..2580)
                     /locus_tag="ACHJWB_002497"
     CDS             complement(580..2580)
                     /locus_tag="ACHJWB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4314114.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSNDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSMVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2577..3713)
                     /locus_tag="ACHJWB_002498"
     CDS             complement(2577..3713)
                     /locus_tag="ACHJWB_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4314115.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3706..5985)
                     /locus_tag="ACHJWB_002499"
     CDS             complement(3706..5985)
                     /locus_tag="ACHJWB_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO4314116.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5996..7084)
                     /locus_tag="ACHJWB_002500"
     CDS             complement(5996..7084)
                     /locus_tag="ACHJWB_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4314117.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(7391..7708)
                     /gene="yehK"
                     /locus_tag="ACHJWB_002501"
     CDS             complement(7391..7708)
                     /gene="yehK"
                     /locus_tag="ACHJWB_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4314118.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEINKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(7770..11402)
                     /gene="yehI"
                     /locus_tag="ACHJWB_002502"
     CDS             complement(7770..11402)
                     /gene="yehI"
                     /locus_tag="ACHJWB_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4314119.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQAQSVVKRYLSASPKPVIDSTDNSGLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTVWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            complement(11412..15206)
                     /locus_tag="ACHJWB_002503"
     CDS             complement(11412..15206)
                     /locus_tag="ACHJWB_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO4314120.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            complement(15347..17380)
                     /gene="metG"
                     /locus_tag="ACHJWB_002504"
     CDS             complement(15347..17380)
                     /gene="metG"
                     /locus_tag="ACHJWB_002504"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4314121.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIIVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            17512..18621
                     /gene="apbC"
                     /locus_tag="ACHJWB_002505"
     CDS             17512..18621
                     /gene="apbC"
                     /locus_tag="ACHJWB_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4314122.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            18884..19165
                     /locus_tag="ACHJWB_002506"
     CDS             18884..19165
                     /locus_tag="ACHJWB_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO4314123.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         4534 a         4906 c         4959 g         4809 t
ORIGIN      
        1 acttctttaa aaccagcggc tttcatcacc agttccattt gcgccatagt gatacctttt
       61 ttggcatctt cagcagaaac gttgattcct gaaataccct gcagggcttt aaaatccact
      121 ttttccatat cgatagtcac gttttcctgc gcgtaggtat cggtataggt taatttttct
      181 tcaacacccg cgatgttttt gtatttggcg cttaacggct caagtgtctt ggcagcatct
      241 tctttggtgg ttgcaccaat ggaggcaaat tgaattttgg tttcagaaga ttgcttaagc
      301 accttgtcac ctttgtagac ataggtaatg gcaatttcag tgccgttcag attggcgctg
      361 aatttcttcg attcttcttt gtcaccgcag ccagcaagag agaaaaccag aacagatgca
      421 acaacgaggg aaaacagctt attgaaagcc ttcatgtaaa actccatttt atttaatcaa
      481 ggaactggtg actctcacca ggggctatat aggatatgcc taataccgtg gcgtgagcag
      541 tccggaactg gagtagaact cttagtaaaa agcactattt catccttgtt gctgaagcat
      601 ggggaataat tgttcgcaaa gtaaaacacc gttattcatt gcttctaccc gtgcctcgct
      661 ttctgtatta cgaaattgtc ccaacacatg tgccagccga taaaaaccga ccgcggagag
      721 gtcattcgcc agcaactctg cctgactaat agcactctgt tcctgatagc gccagccgtt
      781 atggagcagt tgaataagta acgcctggca gcgcatcagc aactgatgag cggtagacgg
      841 cacaggcaaa acgctggcag aaggtagcgg tgccacaggc gcagtttctg cgtccagcgc
      901 ccaggcgcgg gtttttgtca tcatcacccg tggttccagt gtcaattgcc cctctacaaa
      961 gctgacaaag ccagaaacca tactcacggg gtcgtctgtt tgttgcaaaa gcgccgccat
     1021 gcgttcaacg gcaaaaggag aacaggcaga tgccggaagg gataacgtca ggagattatc
     1081 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
     1141 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
     1201 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
     1261 cggcgcgctc aacatttgcc aggcatcagg cgacagcggc acaacgctgc ttaagcggtt
     1321 gcgggtagca aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
     1381 cccgcccgct agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtt
     1441 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1501 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1561 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1621 ccacggcaca ggaggaactt caccaatact gtcgcgccgg gccatctctt gtgcgcaatt
     1681 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1741 gcgggcgtgg aaggcatcaa cgcttgcccg tagctgtcgt agtgattcac tcacccatcg
     1801 ccagttgcag cgttccgccg cctgctgcgc gcgactaaaa gcggcctcgt agtgaataag
     1861 cggctggctg atgccgccca gccataatgc ctggcttaat tgctgaacat attgacgaca
     1921 cgcgttaccc tcgtcgttgg caaacggatc gttagatgat gtgacgtgtt cgctgcgcat
     1981 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     2041 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     2101 gctgctacgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcgcc
     2161 cggcgtacag aacaactcaa tggtgatgcc tttagcgacc agcgcctgtg cgcgtttgcg
     2221 ggtagcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc actcttcttc
     2281 tttttccgta gactgagtgg tggcacaaag tcgttgataa cttaacacca gcatcacgcg
     2341 atgacggcac ataccgttgg ccccgcaact gcactgagcc tctttcagtg cctggccgtt
     2401 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgtcgt tctcatggct
     2461 gatctccggg acgttgccat tttccagttc cttaagactg cgcttaacaa aaccggcatt
     2521 gcttaacgcc gtcagggcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2581 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gcgtcatggc ggctatttgt
     2641 gcgccgacat taaccagcgc ctgggccgta tcgcggtcat agcaaggtgt tgcggtgcta
     2701 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacctgatgc
     2761 gtcagtaatg atgatgaacc cccttcgtaa aaatcgctca cgaggataat gacgcttttc
     2821 gctggttgtt caataagttg ccgaccatac tccacggcac tggcgatatt ggtcccgccg
     2881 cccaactgta ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2941 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca caaacaggcc
     3001 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     3061 accagttgcc attgttcgct ttggcgttta atgcggctgt taaagcgggg ggattcgata
     3121 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     3181 tcaaagtttc gcgccagtgg aataaatgaa cggcgacggc gatcgcggac accagaaaaa
     3241 gcctggcgaa cttcctttgc cagtcgcgcc ataatttctt caacaacctg gcgcactatc
     3301 tggcgggcgg cagccagtac ttcgggattc atcagatgtt tggtgtgcaa aacggcgcgt
     3361 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     3421 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3481 gtgtgaatac tgttgatcca ctcaggggtg gtgagatttg agccacctaa tccaccggag
     3541 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtggtct
     3601 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3661 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3721 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3781 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3841 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3901 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3961 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     4021 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     4081 ttcggcactg gcgttgggat gttccagacg cattagtgcg ccaagaaccg cgccgcgatc
     4141 cagcgccggg gcatgaatcg cctgactgcg tcgctccagt aaggctaccg ccgcagaaag
     4201 cgaaacgccg gataaattaa cgccgctatg tagatcgcgc agaatatggc aaagcgcttg
     4261 ccagctatgc aggtgagcgt gaaactcctt ttcatccggt ctgccgttag attcacacag
     4321 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     4381 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     4441 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4501 ggacgccaga cccgctaacg ccgcttggct aagacatgtg gtgatactgc cgattccacc
     4561 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4621 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4681 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4741 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4801 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcagg tgacatcttt
     4861 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4921 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4981 cggcaacggc gcattcaggg cttctttaat cagcgagcct gctatggcat ccagccagtc
     5041 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagc gccatcgcat gcagatgagc
     5101 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg taaggatagt
     5161 ttttagcagt tgttcaccgg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     5221 cggggcaggc ataccggaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     5281 cgtgagataa caacctgtaa tggcatctgc cagcgagggc aattccggcg tgttaatgtc
     5341 ctgcgggcat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacgac
     5401 taacacgtcg ccattatttt gctgcaccgc ccatgcaatc cagcgagcca taaaggcttc
     5461 gcgctgacga ttgagtgcat cgccggggaa atcgccccgc agttgggcaa aatagtgcgc
     5521 cagagcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5581 atcgctgtta tccatgcggg tggcacgcag cagtaaggcc tggctttcat cttgcgtatc
     5641 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5701 tgcctgaata cgacgtgcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5761 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5821 taccggaagc tggtgggaaa aaaaaagttc gtctacccgg tcattaaaat cagccgggcc
     5881 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5941 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcctc tttcctcacg
     6001 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     6061 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     6121 cggcaataca gtccaccagg tcggcaggct cccccgcgcg attcgctaaa aaccaggcgc
     6181 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgtcagtg
     6241 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cgtaccagta
     6301 actccagtac tgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     6361 aagcactggc gaccagttcc agctcctggg cgaaatccat aatcggaaag acggtttcaa
     6421 aatcgaagcg acgctttagc gcggcactca tctcattgac gccgcgatcg cgggtattgg
     6481 cagtggcaat aatattaaag ccttcccgcg catagagctg actggcttca ccggtgagtt
     6541 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgt acttccagcg
     6601 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc tggtaaagcg
     6661 gggcagggac cagtgcttcc gttgacggac catggttgat aagcagggcg tagttccagc
     6721 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6781 cgctaatagc ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6841 ccaacatcaa accgcgcccg gtggcaagag tgactaacat gcgttcgaca gcggagggac
     6901 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6961 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     7021 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     7081 acatatattc atccctgaaa tgtattaatg aaataaacga tattttacgc gcaataaatg
     7141 tttggcgcat tagttgtttg agcggaaaat atctggaaag cagatcacag taaataaaaa
     7201 aacctgctgg tgaggcaggt tcttttattt actattttca ggcccaacac cgcagcctgt
     7261 gcaacatcgt gttccaacga acgaattata accacggctg gaccccgttg caattaagat
     7321 aatgagattt ataatccagg ccattgggac gttgcgccgg aacggaccaa tttgaattat
     7381 ttagagtata ttattctatt tcttggttga atatattctg tagttcacga ttaaaaattg
     7441 ctattacagt ttcaggtgat tcacctcgta atttccgctc atcaatacac tcatctctta
     7501 tataaatcaa cgcatcattt attagcgggt cagcatcttc tcgatcacgt tgatgaaatg
     7561 ttggacgtag ccggaatcgc tccagtcgat agacggaacc tgtaaataat tcagagcatt
     7621 tattgatctc aagtctgaag gaaaacggat tttcaggtac agggacctca taatcgtaaa
     7681 tagcaaccag ctctttttgc acgatcatcg ctttccccgc tggtaagtcg tgttgataac
     7741 gggcagttgc atcactgccc gctgtgtttt taatggaaaa tgacgtcagg tgcgcttaac
     7801 gcctcactca aatcaaacac atcaagttcg gcaaaagtga cggatttatt gtcacggtac
     7861 actcgaagag aacggaagct cattaacgct ttgctatcaa cattgtagtg gttaaaaccg
     7921 ggtgaaaact taaggaccag ccggtactcg cccaggtcat agacaaatgc gtcctcatag
     7981 cctgactccc agcctttgta tcgcattccc ataagctgcc caacggtagc ggatttacct
     8041 tcccagcgag tcaggctgtt actggttgac tcgtcaggtg tgagcagcac cgtgcggcgc
     8101 gacaactggc gaaaaggcgg cataatttcg taatcggcaa aaagctgtgc aaattcactg
     8161 cgcatttcta ctgtcatttc taacgggtgg gcaatgccaa tcagagcgtc cgcaggcagg
     8221 tcaattggct catcttgcgc attgcagaac tccccctctg cggccacacg aaaggctttg
     8281 agtaaacaac gcggttcatt tgccggataa accccccata ttaatcgctg ggtaaccaga
     8341 cgggtaaagg gatgattaac aaacagagaa tgaaaatctg ccagcgacca gcgtcgggtg
     8401 gtacgtaggg cagcttcaag acgcgggagc aggtttttgc tcacctgagt agcatctttt
     8461 tttagccctt ttagtcgggc cagtgcctcg ggcgctttca gttgatcgtc atcggcgcgc
     8521 aaccggggaa cgcttttctg gcgaacgttt tgctgattaa agataaccgg gttgaggttt
     8581 tcatcgaagc gaacggtaaa ctgccgggga ccaaaatcaa aactcaacgc ctgcggatca
     8641 tcaaggccca gggttgggac taatctgtcc gctagctctt cctggcttag cccgcgattt
     8701 tctgcgacta cctgaagaaa ttccgccgcg ttatcacgta aggggcggga tttagcccgt
     8761 tgcgatatat gatgcagctg tatcagcgcc atatcattat tcatcagggt aaggatgttc
     8821 aggccgctga cagcacgggc agatttgcct tcttgtggcc aggcgaggat ctgtgtcgtc
     8881 agatcccgtg ccgtgctttc gtcaccaaag agacttaacg ccagaaatgc ccagttgtct
     8941 tttgcagggg caccagcttg ttgccacaca gtgaagagat cccaggcaaa agctgccagc
     9001 gtttgtggct ggcagaacgt tttcagttgt tccagcccgc tataaaaacg tcccccctgg
     9061 gtaaagcgca gcatttcgcc gataatttcc agagcgtcac cggtaacagg ctgattatta
     9121 gtgataagtc gaggcctgga ccacattgcc ggatgccaga aatcaggggc ttttggaatg
     9181 cgtgccgggt aaatgtccat tggaccctgt ttaagcaact gctccagggc agaccataca
     9241 tctgtacgct gccagcggtt tgccacggtt tgtagcaatt cgccatgtcc ctgttcgtac
     9301 agtaaacgca gggcaagtaa tgcggcttcg ctattatcgc tgggtttggt aaagacaaga
     9361 gggataagtg cactagccgt atgttccggc cattgtaaaa tccactggcg agccagatca
     9421 cgctgcgccg cgaaacgtcg ccagacgcgg gcaacaggca gcgccagttc tgttgcgcca
     9481 aaatttaaaa ttaacggaaa tgtttctttt ggacgatgtg aaaaagccaa cagcagacct
     9541 ggtagcgcat ccaatccgag gatactgaga aaatagtcct cacccgcgaa cagatgcttt
     9601 ttctcattga tacgcagcca gaaggtcaaa gccatctcac gcggcaactg tgccaataaa
     9661 tacaggttcc agacactctt ttccggacga taacgtgagt agttgtcatt ttcccatatg
     9721 ttaattaacg cttctatgtg accgctgcgg agcgcctgtt ctgctaattg tgcttccggg
     9781 gagttccagc cattcaggat ctcttcaacc cgcccctggt agtaatgcca ctgaacaatg
     9841 agtgaattgc gtgcagcatc cagttttccg gggagtatat aattcagaaa cagccataca
     9901 tcatccccaa aacccagttc ctgtaataca actcgtccac ttttttgttc catgcgttcc
     9961 gcaagagatt ccgtgctgaa ataacgggca gactcagtgg cggcaagcct ctcttgttcg
    10021 cctggttgcc agtaaacttg cggagttaat tcgagcggtg ccaaatcaag acgtggagct
    10081 gtcatttttt tcttactacg ccacggtggt gaaacgagca tttcaggcag accgctgtta
    10141 tcggtgctat caataaccgg ttttggcgat gccgataaat aacgcttcac cactgactgg
    10201 gcttgagtac ttaaccacgg catcaccctt tctgctagtt gtggctgcga tgctatcagc
    10261 gtttgtaaca gcgtacgcca gtattcatct cgagcttttt gctgtgcagg cagctttttg
    10321 tcttcgatga ttggataacc tgggcgggct ggtggttctt ttaacgccag cagttctgcc
    10381 agtgcggcga gtgtcgcatg ggggaagttt ttactgtatt tagccacacg ttgcaggctg
    10441 ggtttgtttt tatcagcaac taacagcaag gtgcggatga cttgcggatg gttaatttgt
    10501 accagcagac tgccgcaatc ttctttatgg gcatatatcg cgaggcgcgg aacggctgcc
    10561 agtccttgtt cgcgcaaaag ggcggcgcag gccgcgtagc cgaagtggtt ttcatgcgac
    10621 atgtagctgg cttctcgatc gctaaatata tcctggctcc agtagcgttc gagttttctc
    10681 accgctttgg ggtcagtagc aacaactttt aaccactcct tagaatgaaa atgagtccgc
    10741 gggcattcaa ggcttaccaa ctcattggcg atttctggtt tttcggggag gatcagcgca
    10801 ataaaagggc ggcgaacttt ggttattcct ggcagtgcgg caatgagttt gtcggcgcaa
    10861 cgctgccagc agctttcctc tgccagagag aggtgtttac gcagtcggag atcgaattcg
    10921 ttataggttt ctgagcgata accatagcca tagtcctcgt tctgatactg caatgtgact
    10981 accagaggat cactctcata acaccgtgca ataaactggc gtgcaatcac cacctcagtc
    11041 gcatattcca gcccctcttg ttggacgatt tcgtcgagca actgttggtt gtcattttgc
    11101 gcgatacagg ctagtgccgc cattgtgcgg gccgggactg agggtttgtg ttcgccgatc
    11161 aagttgatgg cctgcgtaat ggcggactgc cactccagtg aaaaattttc ataacgccag
    11221 acaatgtttt ttttcttctc agctttttgt agcaattcat cacccagtgt ctgaatgatg
    11281 ctttcagtga taacaggcac cggttcgccc ggccatttgc gatgactgag tggtgttttg
    11341 ccttttttat atttcagttc cagttgggcg ttatccgcca gccacggtaa ttccttgtcc
    11401 atgctctttc cttattcaaa cagggcttca atatcgttaa ttagctcgct ggcggtgatg
    11461 gcatcgagta cggagaactg cgcttcctgt gttgaattac tcccccagcc atagctttcc
    11521 gctttgcctt cccataacca cagcttgctt aagagctgct cagcgctaag ttcggctggt
    11581 gacataccca ccgcaaaacc ttcgtcgatt tccatgatta acgaccagcg acccaaaggt
    11641 ttgatcatcc atccgatcca gcctccatcc tgtggttcgc cctttatcca gcctttattc
    11701 gccagcccca tgacccgacc actcgggcat tttctgcctg cccagcgggt cagttcactg
    11761 gcattgcgct cggcttctgt cagggcgtag ctgttacggt cgagctggcg gaacggtggt
    11821 agcagttcgt agtcggcaaa aagctgacca aaggcggcag catccgttgg tgatatttcc
    11881 agaacgtgag gagtgccgat agagatatcg ccttccggca gggtgaaaag atcatcgtca
    11941 gcggtgctgt agctgttatc ttctgctacg cgaaagcaag ccagtagctg gttttcggcg
    12001 ctataaacgc cccaaatcag acggcgggtt aagtgacgaa ccagcggatg ctcaaccagg
    12061 aagagctgga agttttccag cgaccagcgg cggcgcaggc acatggcgga ttccagcctt
    12121 gctacctgct gggcggcgat ggtacgcgca tcttttttca gcaatttgta gcggttaacc
    12181 gcatcgttcg cccgcgtttc atcatcgctt ttgttcggtt tgggcaggtc tttcaggcgg
    12241 ctgccggaaa catcacgcac aaacggtttt aaggtttcat caaagctgac ggtgaactgc
    12301 cgtgggccaa aatccagcag cagcgaaccg ttatcatcca gaccgagatc cggtgctaac
    12361 cgatcttcaa gctccgccac cgtgagttcg cggctctcgg caatgtcggc aattttttct
    12421 tttgcccgct cctgtaatgc tttgaatttc agtttctggg cgatgccgtt aagctgcata
    12481 agggcgatat cactaccgat agcagcgaga atatccaacc caacggtggc gcgtttatgc
    12541 tgggattcac caggccaggc gcgtattaat ggcgtcagtt tgcgggcggt gtcatcgtta
    12601 ccgagaacgc ctaacgcagt gaacgcccaa ctctctttcg acggcgcgcc agcggtctgc
    12661 caggcggtaa acagatccca ggcaaatccc gccagtgagt cggcggaaca gacgtctttc
    12721 acctgcaata atcccggata cagagcctct tcctgaggga agcggagcat ttcaccgagg
    12781 tggaggaggg cgctatctgg cagtgattgg gcatttgctt ttaataccgg gcgcgtccag
    12841 agcgatggct gataaaaggc cggaagagtg gggatttttg tcgggtgatt atctaaggga
    12901 tcgagcgcaa gcagagcgtt caccgcatcg gttacttccg gctggttata acgtcgggcg
    12961 atttcttgca gtaatggctg atgaccgttt tcggtaagca tacgcaaggc agcgcgggcg
    13021 ttatcctgtg cttcaccggc tttgccgagc gccgcaggca gcaggccggt aagggcatgt
    13081 tccgggtatt tcaacagcca gctacgggcg ttttcgcgta gcgttttcag tttgttgaag
    13141 gcacgggcga cggcaggagc cagctcactc gctgcgaagt aattcgtgat gggcaaggct
    13201 tcttgtggat agcgtgcaag tgagtttact aacccaggta accctttgag tccaaaatgt
    13261 aacatggcgt attcggtatc ggtatgtgga gatagggtaa gggcattcca tgcttttact
    13321 gctttctcca gcggcaatgt tgtcaaaatc cccaactgcc aataatgccc ggtaggtgaa
    13381 tgaaacacct taaattcact tagtaaggtt gaataatctt catttagcca tgcctcaact
    13441 accgcttcgg gagctttaat atatgtacca gcgatccgtc gttgaaaacc catccttgcc
    13501 aacaggttgg tagtactttc ttcatggcta accgtgatgt gttttgaata ccagatatat
    13561 ttcgccaaaa gttgattact gatttcttct gtcagataac agattggctc aatgcctaat
    13621 ggcgctaaat ccagcaccgg aatcggcgat tttttctttt tcgaaagcca gggaggggag
    13681 actactacgg ctggcagtag atcggcgctg gcatggtttg agggctgtgt cagttgttgc
    13741 tggcatgatt tcagtacggc aactgcgggt gtcgagagcc agggaatgac ctgttctgcc
    13801 agtgctggtt gtgagataag cattgtcatt agcataatgc gccaactgtt ctcttctttt
    13861 tgcccaagaa gttccgtcag tgccgccatt gctgcatgtg ggaacgcagc aatggctttc
    13921 gtcatccgat cgtgacagcg tttagtatgc ccggccacac gtataagcag tgttaatgcg
    13981 aacggatgat tgatatgacg caacacatcg gcacagtagt cacttgcggc atagggagca
    14041 aatcggggca gggctgcaac accttgctcc tgtaatacgg tggctgacca tatcgcacca
    14101 caatagtaat catcaaaaat ggcatatggc tcgtgatatt tttctaatga ggcaagaatg
    14161 tgctcatcag tcgccactat ttttaaccac tcaagcgagg gtaatttttt ttgccccagt
    14221 agacgctggg caatttcatg tgcaatttct ggtttttcgg gtaataacaa agcaattaat
    14281 ggttgccgcc attctggaat atgtggaatt gcggcaataa gtttttgtgc acatttttgc
    14341 cagagagact cttccgtcag tgataaatgt ttacgtaggc gtaattcaaa tctggaatat
    14401 tgctccaagt agctaggtgc aatgccagac ggcagaataa taatgacatt attcgcataa
    14461 tcccattcaa tatcaatctg ttgaaggtgt attattacat cagtagcata ttccagaccg
    14521 ccttcctgga cgatctgatc cataacatcc tcacgcactt tatatcttgt gcatttcatt
    14581 tcaagtgccg tgagtacagc cattactggt gatggtaact gtgttgtttt caggccgtcg
    14641 atctgactga tcgcctgggc gacggtgttt tgccattcta aagaacaggc gctgaagtca
    14701 aatgtggctg ttttattatc ccgttgcgac acactacagg ctaagcgacg taattgggtc
    14761 aatttgtctg cttgctgaac aggaactccg ggaaagcggc gatgacttaa cgtggtgtgt
    14821 acaacttcgg gcggaaaaac gacagcaata tctttcgcca gccacggtaa aacagcatct
    14881 gtatttttgt caggcgcacg ggtttctgct acgaaagagg gttgttcctg aataatggtg
    14941 gataagtccg ctaccttgag agagcccgtt accgtttggg aaggttgcaa agaattatcc
    15001 ttcgcttgct ccacataccc cttcttcacc ttctccgcaa tcagcttaag ctccgctttt
    15061 gccgctgccg cagcatctga aaaacttttt atctggcttt gccctttggt gccaactttt
    15121 ccccaactga tatgcaactc gtttccctgt tgctcaactg cccagaattt atgtgatttt
    15181 tcgtcctgat agataaagtg tctcattctg catccctgta attttatcca gttgaatgca
    15241 gatgctacca gtatttatgc gggttagaga gagacaaaat cgcaagaaaa caactttagg
    15301 aatttataaa gaaaaggcgc tgtcgatgca gcgccttgaa gggggattat ttcacctgat
    15361 ggcctggttt agcaccagca tccgggctta acaggaagat atctttcccg ccaggaccag
    15421 cagccattac catgccttcg gagataccaa agcgcatttt acgcggtgcc aggttggcca
    15481 ctataatggt gtgacgcccg atcagtgcct gcggatccgg gtaggcagaa cgaataccgg
    15541 agaagacatt gcgtttttca ccgccgagat ccagtgtcag gcgcagcagt ttgtcagaac
    15601 cttcaacaaa ctctgcgttt tcgatcagcg ccacgcgcag gtcaactttt gcgaagtcgt
    15661 caaaggtgat ggtttcctga atcggatcat ctgccagtgg gccagtaact ggcgcggcag
    15721 ctgctttcac ttcttcttta gacgcttcca ccagtgcttc aacctgcttc atatcgatgc
    15781 ggttatacag cgctttgaac ggattcactt tgtggcccag cagcggttgc tggataccat
    15841 cccaggtcag ttctgtattg aggaatgctt ctgcgcgctc ggtcagtttc ggcagtaccg
    15901 gcttcaggta agtcatcagc acgcggaaca ggtttatgcc cattgagcaa attgcctgca
    15961 ggtcggcatc gcggccttcc tgttttgcca ccacccacgg agcctgctcg tcgacatagc
    16021 ggttagccag atcagccagc gccatgattt cgcgcactgc tttaccaaat tcgcggcttt
    16081 cccacgcttc accaatcact tcagcggcat cagtgaaggt tttgtacaac tgcgggtcag
    16141 ccagttcgct tgccagcacg ccgtcaaaac gcttgttgat aaagcccgca ttacgagagg
    16201 ccaggttaac cactttgttc acgatatcgg cattcacacg ctgaacgaaa tcctccaggt
    16261 tgagatcgat atcatcaatg cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt
    16321 cagcgtcaaa atgattcagc caggtgctgg ctttaataaa ggtgccgcga gacttggaca
    16381 tctttgcgcc gttcaccgtc acatagccat gaacaaacag gttggtcggc ttgcggaagt
    16441 tgctgccttc cagcatggca ggccagaaca ggctgtggaa gtaaacaata tctttaccga
    16501 tgaagtggta cagctcggcg gtggagtctt tcttccagta ttcatcgaag cttacgctgt
    16561 cgccgcgctt gtcgcacaga ttcttgaaag aacccatgta gccaatcggt gcgtccagcc
    16621 agacgtagaa atatttgccc ggcgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc
    16681 gggagatatc ccactgttgc aggccggatt caaaccactc ctgcatttta tttgccacct
    16741 gctcctgcaa cgcaccgctg cgggtccatg cctgcaacat ttcgctgaaa gagggcagat
    16801 caaagaagaa gtgttcagaa tcacgcatta ccggcgtagc gccagaaacc accgatttcg
    16861 gctcgatcaa ttcagtcggg ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt
    16921 gatccgggga tttacatttc gggcaggtgc ctttcacaaa acggtccggc aggaacatgc
    16981 ctttttccgg atcgtacagc tgagagatgg tgcggttttt aataaaaccg ttttctttca
    17041 ggcgagagta gataagttct gacaactggc ggttctcttc gctgtgcgtc gagtgatagt
    17101 tgtcatagct gatgttaaag cctgcgaaat cagtctgatg ctcctgactc atttcgccaa
    17161 tcatctgctc cggggtgata ccgagctgct gagctttcag catgatcggt gtaccgtggg
    17221 catcgtcggc gcagatgaag ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga
    17281 catcagcctg gatgtgctcc agcatatggc cgaggtggat tgagccgtta gcgtacggca
    17341 gtgcgcacgt caccagaatt ttcttcgcga cttgagtcat agtaggcatt acttcttaat
    17401 agtgaaaagg ggttagatag taccaaatgg gaaaatgtta agtaagtact tctctatatt
    17461 cttcaggtaa actatcagct gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa
    17521 caatcccagg ccaaatcacc agaagccttg cgcgcaatgg tcgccgggac actggccaat
    17581 tttcagcacc caaccctgaa gcataacctc accacgctga aagcgttgca ccatgtcgcc
    17641 tggatggacg acacgttgca tgtggaactg gttatgccat tcgtctggca tagcgcgttt
    17701 gaagagttaa aagagcaatg tagcgccgaa ctgctgcgta tcaccggggc gaaagctatc
    17761 gactggaaac tgtcgcacaa tatcgccacc ttaaaacgcg tgaagaatca gccgggtatt
    17821 aatggtgtga agaacatcat tgcggtcagt tccggtaaag gcggcgtggg taaatcctcc
    17881 acggcggtaa acctggcgct ggcactggcg gcggaagggg cgaaagtcgg cattctggac
    17941 gccgatatct acggcccatc aattcccacc atgctgggcg cagaaaatca gcgtccaacg
    18001 tcgccggacg gtacccacat ggcaccgatt atgtctcatg gcctggcaac caattcaatt
    18061 ggttatctgg tcactgacga caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg
    18121 ttaatgcaga tgttgcagga aaccttgtgg ccggatctcg actatctggt gcttgatatg
    18181 ccgccaggca ccggtgacat tcaattgacg ctggcgcaaa acattccagt aactggtgca
    18241 gtcgtggtca ccaccccgca ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg
    18301 ttcgagaaag tcgaggttcc ggtgcttggt attgtcgaga acatgagtgt gcatatttgc
    18361 agtaactgtg gtcaccatga accgattttc ggtaccggtg gtgctgagaa actggctgaa
    18421 aaataccaca cacagctgct ggggcagatg ccgttacaca tctccttacg tgaggatctc
    18481 gataaaggca cgccaacggt tatcagccgt ccggagagcg aatttaccgc tatctaccgc
    18541 cagctggctg accgcgttgc agctcagctc tactggcagg gtgaagtcat tccaggcgag
    18601 atttccttcc gcgcggtcta acgcctatta accattctgg ttattcttca tacaccgcag
    18661 agatgttaca ttgatgcggt gtattagttt ttgccgcatt aatgaacact tatttaatac
    18721 tgcgaatatt ctgcggacct tcgtccgaaa ctctacaaag tcgattgatc atacaggcaa
    18781 tgcttcatta tcatccttcg tggctgtgta gtggatgtac agctatagtt tgttatgcac
    18841 tcattctatc caggcataac ctttactaca gggataacgt tcaatgaata agtattggct
    18901 gtcaggaatt atttttcttg cctatggatt ggcttctccc gcattctctt cagagactgc
    18961 cacattagcg attaatggca ggatcagtcc cccaacatgc agtatggcta tggttaatgg
    19021 tcaacctcaa cagcattgtg gtcagttaac gtataatgtt gacacccgtc atctgttttc
    19081 ttcgcccgtc aaaggtgtaa cgacagaagt ggttgttgca ggtagcgata gcaaacgtcg
    19141 aatcgtgtta aatcgctacg attaatttgg caacctgatt ttaattgcat ttagaaagcc
    19201 gaaatcat
//
LOCUS       DAZFAH010000075        19176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFAH010000075 DAZFAH010000000
VERSION     DAZFAH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.075"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..269)
                     /gene="tccP2"
                     /locus_tag="ACHJWB_002507"
     CDS             complement(<1..269)
                     /gene="tccP2"
                     /locus_tag="ACHJWB_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO4314124.1"
                     /translation="MINSINSFFSGLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFS
                     PSNSPASATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPLPDV"
     gene            complement(872..959)
                     /locus_tag="ACHJWB_002508"
     tRNA            complement(872..959)
                     /locus_tag="ACHJWB_002508"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Ser,seq:tga)
     gene            1386..2504
                     /gene="hyaA"
                     /locus_tag="ACHJWB_002509"
     CDS             1386..2504
                     /gene="hyaA"
                     /locus_tag="ACHJWB_002509"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4314125.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2501..4294
                     /gene="hyaB"
                     /locus_tag="ACHJWB_002510"
     CDS             2501..4294
                     /gene="hyaB"
                     /locus_tag="ACHJWB_002510"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4314126.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4313..5020
                     /gene="hyaC"
                     /locus_tag="ACHJWB_002511"
     CDS             4313..5020
                     /gene="hyaC"
                     /locus_tag="ACHJWB_002511"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4314127.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5017..5604
                     /gene="hyaD"
                     /locus_tag="ACHJWB_002512"
     CDS             5017..5604
                     /gene="hyaD"
                     /locus_tag="ACHJWB_002512"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4314128.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5601..5999
                     /gene="hyaE"
                     /locus_tag="ACHJWB_002513"
     CDS             5601..5999
                     /gene="hyaE"
                     /locus_tag="ACHJWB_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4314129.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5996..6853
                     /locus_tag="ACHJWB_002514"
     CDS             5996..6853
                     /locus_tag="ACHJWB_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4314130.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6987..8531
                     /gene="appC"
                     /locus_tag="ACHJWB_002515"
     CDS             6987..8531
                     /gene="appC"
                     /locus_tag="ACHJWB_002515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4314131.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8543..9679
                     /gene="appB"
                     /locus_tag="ACHJWB_002516"
     CDS             8543..9679
                     /gene="appB"
                     /locus_tag="ACHJWB_002516"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4314132.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9692..9784
                     /gene="cbdX"
                     /locus_tag="ACHJWB_002517"
     CDS             9692..9784
                     /gene="cbdX"
                     /locus_tag="ACHJWB_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4314133.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9864..11162
                     /gene="appA"
                     /locus_tag="ACHJWB_002518"
     CDS             9864..11162
                     /gene="appA"
                     /locus_tag="ACHJWB_002518"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4314134.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11277..13457)
                     /gene="etk"
                     /locus_tag="ACHJWB_002519"
     CDS             complement(11277..13457)
                     /gene="etk"
                     /locus_tag="ACHJWB_002519"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4314135.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13477..13923)
                     /gene="etp"
                     /locus_tag="ACHJWB_002520"
                     /gene_synonym="yccY"
     CDS             complement(13477..13923)
                     /gene="etp"
                     /locus_tag="ACHJWB_002520"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4314136.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13911..15050)
                     /locus_tag="ACHJWB_002521"
     CDS             complement(13911..15050)
                     /locus_tag="ACHJWB_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4314137.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15096..17192)
                     /locus_tag="ACHJWB_002522"
     CDS             complement(15096..17192)
                     /locus_tag="ACHJWB_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4314138.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLNGGIFTNTYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRNWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17192..17938)
                     /locus_tag="ACHJWB_002523"
     CDS             complement(17192..17938)
                     /locus_tag="ACHJWB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HFO4314139.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17935..18579)
                     /gene="gfcB"
                     /locus_tag="ACHJWB_002524"
     CDS             complement(17935..18579)
                     /gene="gfcB"
                     /locus_tag="ACHJWB_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4314140.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTCRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18686..18991)
                     /locus_tag="ACHJWB_002525"
     CDS             complement(18686..18991)
                     /locus_tag="ACHJWB_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO4314141.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         4439 a         4919 c         5087 g         4731 t
ORIGIN      
        1 acatcaggta gaggccgatg taaagcgttt ttttctatac ttgactgagt ataagatgtg
       61 gggcgttgaa gtcctgattc tgtataagaa ttttttacct taaaaatagt tgttgcactg
      121 gcaggactgt tgctgggaga aaaaggtgag ctggttttta ccgtgttagg tgtgcttttg
      181 tgctgcgaga cagtgaaggt actgctgcgt atagcacttg atatactgcg aggaaggccg
      241 gaaaaaaaag aattaatgct atttatcata tagataacct tataagagct ttattggata
      301 gtcttattta tccgtttaca tgaaatctac cttttgtgaa attctcgtca atattttact
      361 caaaatgatc ggctgtagga ataaactatt tgatattatt acgtttttag agttgttttc
      421 aatcagctat agatagggct tggcgccaga atggagaata tttaacgagg aatagaagct
      481 ttgtatcaga cgggcttccc taaatagaga agttaaataa tcaggatgat gctaatgaat
      541 atcagggaca aggtgagtgt cttcatgttc agtgaaacag ggtgtcacaa aaaagattgg
      601 ggagggctag agccttctgc acgaaaatag tgatgcgaca gatctgagat ggctttgtat
      661 ccttctgcct ggttcaggcc tggagcagtc catgttttac aaaaatactc ggcagcgtaa
      721 cgtgttgcaa gagcggcatc agacgaggaa cctgaggttc cgctcatgac cgataatctc
      781 atccgaataa aaatatacca attggtatac caagtttata ccaagagcca aaaaagcaaa
      841 ggggctacca tttggtaacc ccttgattat ttggcggaag cgcagagatt cgaactctgg
      901 aaccctttcg ggtcgccggt tttcaagacc ggtgccttca accgctcggc cacacttccg
      961 gaatgacgcg cactataaac atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc
     1021 ctgaaaaaca gccaaaagtg cattgatagc gtgaaataac agcagattga tcatttcatc
     1081 accatgaatt ccttctcttt tactcgttta gcaaccggct aaacatcccc accgcccggc
     1141 aaaaagaaaa ataggtccat ttttatcgct aaaagataaa tccacacagt ttgtattgtt
     1201 ttgtgcaaaa gtttcactac gctttattaa caatactttc tggcgacgtg tgccagtgca
     1261 gaaggatgag ctttcgtttt cagcatctca cgtgaagcga tggtttgcct tgctacaggg
     1321 acgtcgcttg ccgaccataa gcgcccggtg tcctgccggt gttgctagga gaagagacgt
     1381 gcgatatgaa taacgaggaa acattttacc aggccatgcg gcgtcagggc gttacccggc
     1441 gcagctttct caaatattgt agtctggctg ccacgtcgct gggattaggc gcgggaatgg
     1501 caccaaagat tgcctgggcg ctggagaaca aaccccgcat tccggtggta tggatccacg
     1561 gtctggaatg cacctgctgt accgaatctt ttatccgctc cgctcaccca ctggcgaagg
     1621 acgtcatcct ttccctgatt tccctcgatt atgacgatac tttgatggct gccgccggaa
     1681 cccaggcgga agaagtcttc gaagacatca tcacgcaata caatggcaaa tatatcctcg
     1741 cagtagaagg taatccgccg ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac
     1801 cgtttattga gaaactcaaa cgtgccgctg ccggagccag cgcgattatc gcctggggaa
     1861 cctgcgcgtc ctggggctgc gtgcaggccg cgcggcccaa tccgacgcag gcaacgccta
     1921 tcgacaaagt catcaccgac aaacccatta tcaaagtacc tggctgcccg ccgatcccgg
     1981 atgtgatgag cgccatcatt acttacatgg tgacctttga tcgcttgcca gatgtcgaca
     2041 gaatgggccg tccgctgatg ttctatggtc agcgaatcca cgataaatgc tatcgccgcg
     2101 cccacttcga cgccggagag ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt
     2161 actgcctgta caaaatgggc tgcaaagggc ctaccaccta taacgcctgt tcctccacac
     2221 gctggaatga tggcgtttct tttccaatcc agtctggtca cggctgcctg ggctgtgcgg
     2281 aaaatggttt ctgggatcgc ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg
     2341 gtactcattc cactgccgat accgtcggtt taaccgcgct tggcgtggtg gcagcggctg
     2401 ttggtgtgca cgcagtcgcc agcgccgttg accagcgcag acgtcataac cagcaaccta
     2461 cagaaaccga acatcagcca ggcaatgagg ataaacaggc atgagcactc agtacgaaac
     2521 tcagggatac accatcaata atgccggacg ccgcctggtg gtcgacccga ttacgcgcat
     2581 cgaaggccac atgcgctgcg aagtgaatat taacgatcag aatgtgatca ccaatgccgt
     2641 ctcctgcggc accatgtttc gcgggctgga gatcattctg caagggcgcg acccgcgcga
     2701 tgcgtgggcg ttcgttgaac gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc
     2761 ggtttacgcc atcgaagatg ctatcggtat taaagtgccg gacaacgcca atatcatccg
     2821 caacattatg ctggcaacgc tctggtgcca cgatcatctg gtgcacttct atcagcttgc
     2881 cgggatggac tggatcgatg tgttagatgc gctgaaagcc gacccgcgga aaacctccga
     2941 actggcgcaa agtctctcct cttggccgaa atcatccccg ggctatttct tcgacgtaca
     3001 aaaccgcctg aaaaaattcg ttgaaggcgg gcagttgggg atcttccgca atggctactg
     3061 ggggcacccg cagtacaagt tgccgccaga agccaacctg atgggctttg cccactacct
     3121 cgaagctctc gatttccagc gtgaaattgt gaaaatccac gcggtctttg gcggtaaaaa
     3181 cccacatcca aactggattg tcggcgggat gccttgcgcc attaacattg acgaaagcgg
     3241 cgcggtcggg gcggtcaata tggaacgcct gaacctggtg cagtcgatca ttacccgcac
     3301 ggcggatttc attaacaacg tgatgatccc cgacgcctta gccatcggtc agttcaacaa
     3361 accgtggagc gaaatcggca cgggtctttc tgataaatgc gttctcagct acggcgcatt
     3421 cccggatatt gccaacgact ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat
     3481 taacggcgac ttcaacaatg tgctgccagt ggatttggtt gatccgcagc aggtgcagga
     3541 gtttgtcgac cacgcctggt atcgatatcc caacgatcag gtcgggcgtc atccgttcga
     3601 tggcatcacc gacccgtggt acaaccccgg cgatgtcaaa ggcagcgata ccaacattca
     3661 gcagctgaat gaacaggaac gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc
     3721 gatggaagtg gggccgctgg cgcgcacgtt aatcgcttat cacaaaggcg atgctgcgac
     3781 cgttgagtcg gtcgatcgca tgatgtcggc gttgaacctg ccgctttccg gtatccagtc
     3841 aacgttaggc cgcattttgt gccgcgcgca cgaagcgcag tgggccgcag gtaagttgca
     3901 gtatttcttc gacaagctga tgaccaacct gaaaaacggc aatctcgcca ctgcttccac
     3961 ggaaaaatgg gaacctgcaa cctggccgac agagtgccgt ggtgtcggtt ttaccgaagc
     4021 gccgcgcggg gcgttaggcc actgggccgc cattcgcgat ggcaagattg atctctacca
     4081 gtgcgtggtg ccgaccacct ggaacgccag cccgcgcgat cccaaagggc agattggcgc
     4141 ttatgaagcg gcgctgatga acaccaaaat ggcgatcccc gagcaaccgc tggagatcct
     4201 gcgtactctg cacagctttg acccgtgcct cgcctgttca acacacgtgc tgggcgacga
     4261 cggtagcgag ctgatctccg tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca
     4321 gaaaagcgac aacgttgtca gccactatgt ctttgaagcg ccagtccgca tctggcactg
     4381 gttgacggtg ttatgcatgg cggtgttgat ggtcaccgga tactttatcg gcaagccgct
     4441 accttccgtc agcggcgagg cgacgtatct gttctatatg ggctacatca ggttaattca
     4501 cttcagcgcc gggatggttt ttaccgtggt tttgctgatg cggatctact gggcttttgt
     4561 tggcaatcga tactcccgcg agctgtttat cgtgccggta tggcgtaaaa gctggtggca
     4621 gggcgtgtgg tatgaaatcc gctggtatct gtttctggca aaacgtccga gtgccgatat
     4681 aggccataat cccatcgccc aggcggcgat gttcggctat ttcctgatgt cggtctttat
     4741 gatcatcact ggttttgcgc tgtacagcga acacagccag tacgctattt ttgcgccgtt
     4801 ccgttatgtg gtggaatttt tctactggac gggtggcaac tcaatggaca ttcacagctg
     4861 gcatcggctg gggatgtggt tgattggcgc gtttgtgatc ggtcatgtct acatggcgct
     4921 gcgtgaagac atcatgtccg acgacacggt gatctccacc atggtcaacg gctaccgtag
     4981 ccacaaattt ggcaaaataa gtaacaagga gcgttcatga gcgagcaacg cgtggtggtc
     5041 atggggctgg gcaacctgct gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg
     5101 ctgtatgccc attaccactg gcccgagtat gtggagattg tcgatggcgg tactcaggga
     5161 ctgaacttgc tggggtatgt cgaaagcgcc agccatctgt tgattctcga tgccattgac
     5221 tacgggctgg aacctggaac gctgcgaacc tatgccggag aacgcattcc ggcttatctc
     5281 agcgcgaaga aaatgagcct gcatcagaac agtttctccg aagtgttggc gctggcggat
     5341 atccgcggac atctgccagc acatattgcc ctcgtcggtc tgcaacccgc aatgctcgac
     5401 gactacggcg gtagcctgag cgaactggca cgggagcaac tgcccgctgc ggaacaggcg
     5461 gcgctggcgc agctggctgc gtggggaatt gtgccgcaac cggctaatga atcgcgctgt
     5521 ctcaattatg actgtctgtc gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg
     5581 atgacacagg aggagcaggg atgagcaacg acacgccatt tgatgcgttg tggcaacgaa
     5641 tgctggcgcg cggctggacg ccagtcagtg aatcccgtct tgacgactgg cttacgcaag
     5701 cgccagacgg cgtggtgtta ttaagcagtg acccgaaacg cacgccagag gtcagcgata
     5761 atccggtaat gattggcgaa ttactgcgcg agtttcccga ctatacatgg caggtggcga
     5821 ttgctgacct tgagcagagc gaagccatcg gcgatcgctt tggcgtcttt cgctttcctg
     5881 ccactttagt gtttaccggc ggaagctatc gcggcgtgct gaatggtatt cacccgtggg
     5941 cggaactgat aaacctgatg cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag
     6001 cgaaactttt ttccatctgc tggggccagg aacgcaaccg aacgatgaca gtttcagcat
     6061 gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca
     6121 atgtgctcac agcccgcagg tgattgcgct gttaaacgag ttacaacatc aactaagcga
     6181 acgccaaccg ccgttgggcg aggtgctggc agtcgatctg ttaaatctca acgccgacga
     6241 tcgtcacttt atcaatacgc ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc
     6301 tgacgacagt gaaagtgaaa tacaggaggc gatcttctgc ggattatggc gggtgcgcag
     6361 acgtcgcggc gaaaagttgc tggaggacaa actggaggct ggctgcgcgc cgctggcgtt
     6421 gtggcaggcg gcaacgcaaa atctcttgcc gacagattcg ctgttaccgc cgcccattga
     6481 tggcctgatg aatggcctac cgttggcgca tgagttactg gcacatgtac gtaaccccga
     6541 cgcgcagccg cacagcatta atctgacgca attacccatc agcgaggctg atcggctttt
     6601 tctctcacgt ctctgtgggc cgggaaatat tcagattcgt accattggct atggcgagag
     6661 ctatatcaac gccacggggt tacgccatgt ctggcattta cgctgtacgg acaccttaaa
     6721 aggcccgtta ctggaaagtt atgaaatctg cccaataccg gaagtggtgc tggcagcgcc
     6781 agaagatttg gtcgactctg cgcagcggct tagcgaggta tgtcagtggc tggcggaagc
     6841 tgcaccgacg taaaaagacg gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg
     6901 aatacctctg gtcgtagagt ttcaggataa agagggagat ctaccattat cgggttattt
     6961 ttctctcttc gcctacagga gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt
     7021 ttgctctgac cgcgctgtat cactttttat ttgtacccct taccctgggg ctgatttttt
     7081 tgctggctat catggaaacc atttacgtgg tcaccggcaa aacaatctac cgcgatatga
     7141 cgcgcttctg gggtaagctc ttcggtatca attttgctct tggcgtggct accggcctga
     7201 ccatggagtt tcagtttggt actaactggt cattctattc caactatgtg ggcgatattt
     7261 ttggcgcacc actggcgatg gaagcattaa tggccttctt cctcgaatcc acctttgtcg
     7321 ggctgttctt cttcggctgg caacggctga ataaatacca gcacctgctg gtgacgtggc
     7381 tggtggcgtt cggttcaaat ctctctgcgt tgtggatatt gaatgccaac ggttggatgc
     7441 aatacccgac cggtgcgcat tttgatatcg acaccctgcg tatggagatg accagcttca
     7501 gcgagctggt ctttaatccg gtcagccagg tgaaatttgt gcacaccgta atggcgggct
     7561 acgtgaccgg ggccatgttt attatggcga tcagcgcctg gtatttactg cgcggacggg
     7621 agcgcgatgt cgcattacgc tcgtttgcca tcggttccgt cttcggtact ctggcgatta
     7681 tcggtaccct gcaactcgga gacagttctg cgtatgaagt cgcgcaagta caaccggtaa
     7741 aactggcggc gatggaaggg gagtggcaaa cggaacctgc acctgcaccg ttccatgtgg
     7801 ttgcctggcc ggaacaggat caagagcgta acgcctttgc cctcaaaatt cccgcgctgc
     7861 tagggatcct cgccactcac tcattagata aacccgtgcc gggtctgaag aatttgatgg
     7921 ctgaaaccta cccacgcttg caacgcggac gtatggcctg gctgttaatg caggaaatat
     7981 cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg ggaactggaa ggtgacctgg
     8041 gctacggcat gttgctctcc cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt
     8101 accaggcggc gatgcgtggc gcgatacctc aggttgcgcc ggtattctgg agtttccgca
     8161 tcatggtggg ctgtggttcc ctgctgctac tggtgatgct gattgcgctt gtccagacgc
     8221 tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat ggcgctctgg agtttgccgt
     8281 tgccgtggat tgcgattgaa gccgggtggt ttatgaccga gtttggtcgt cagccgtggg
     8341 cgatacagga catcttaccg acatactccg cgcactccgc tttaaccaca ggacaactgg
     8401 ctttctcact gatcatgatc gtagggcttt acaccctgtt cttaatcgcc gaagtctacc
     8461 tgatgcagaa atatgcccgt ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc
     8521 aacaggggta aaggagaaaa tcatgtttga ttatgaaaca ttgcgcttca tctggtggct
     8581 gctgattggc gtgatcctgg tggtctttat gatctccgac ggatttgaca tggggatcgg
     8641 ctgtctgctg ccgctggtgg cgcgtaatga tgatgaacgc cggatagtga taaacagcgt
     8701 tggtgcacac tgggaaggca accaggtctg gttgatcctc gctggtgggg cattatttgc
     8761 cgcctggccc agagtgtatg cagcggcgtt ttccggcttt tatgtggcga tgatcctggt
     8821 gctgtgctca ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc
     8881 acgctggcgt aaaatgtggg acgccggtct ggtcatcggc agtctggtgc cgccggtagt
     8941 cttcggtatc gccttcggca acttgttgct cggcgtgccg tttgccttca caccgcaatt
     9001 acgcgtggag tatctcggca gcttctggca actgctgacg ccattccctt tattgtgcgg
     9061 attgctcagc cttgggatgg tgattttgca aggtggcgtc tggttacaac tgaaaactgt
     9121 tggtgtgatt catctgcgtt cacagctggc caccaaacgc gctgcactgt tggtgatgct
     9181 gtgctttttg ctggcgggtt actggctgtg ggtcggtatt gatggctttg tactgctcgc
     9241 ccaggatgct aacggtcctt ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc
     9301 ctggatgaat aatttcgtcg aatcgcccgt tttgtggatc ttcccgctgc tggggttctt
     9361 ctgcccattg ctgacggtga tggcgattta tcgtggtcgc ccgggttggg gatttttaat
     9421 ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc atcacgctgt tcccctttgt
     9481 catgccgtca agcgtgagtc caatctccag cctgacgttg tgggacagca cttccagtca
     9541 gctgacgctg agcattatgt tggtaatcgt gctgatattt ttgcccattg tgttgctcta
     9601 cactctctgg agctactaca aaatgtgggg acgcatgaca acagaaactc tccgccgtaa
     9661 cgaaaacgag ttgtactaag gagcaggaac aatgtggtat ttactttggt tcgtcggcat
     9721 tttgttgatg tgttcgctct ccacccttgt gttggtatgg ctggacccgc gtctgaaaag
     9781 ttaacgaacg taggcctgat gcggcgcatt agcatcgcat caggcaatca ataatgtcag
     9841 atatgaaaag cggaaacata tcgatgaaag cgatcttaat cccattttta tctcttctga
     9901 ttccgttaac cccgcaatct gcattcgctc agagtgagcc ggagctgaag ctggaaagtg
     9961 tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg
    10021 atgtcacccc agacgcatgg ccaacctggc cggtaaaact gggttggctg acaccgcgcg
    10081 gtggtgagct aatcgcctat ctcggacatt accaacgcca gcgtctggta gccgacggat
    10141 tgctggcgaa aaagggctgc ccgcagtctg gtcaggtcgc gattattgct gatgtcgacg
    10201 agcgtacccg taaaacaggc gaagccttcg ccgccgggct ggcacctgac tgtgcaataa
    10261 ccgtacatac ccaggcagat acgtctagtc ccgatccgtt atttaatcct ctaaaaactg
    10321 gcgtttgcca actggataac gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt
    10381 caattgctga ctttaccggg catcggcaaa cggcgtttcg cgaactggaa cgggtgctta
    10441 attttccgca atcaaacttg tgccttaaac gtgagaaaca ggacgaaagc tgttcattaa
    10501 cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg
    10561 taagcctcgc atcaatgctg acggagatat ttctcctgca acaagcacag ggaatgccgg
    10621 agccggggtg gggaaggatc accgattcac accagtggaa caccttgcta agtttgcata
    10681 acgcgcaatt ttatttgcta caacgcacgc cagaggttgc ccgcagccgc gccaccccgt
    10741 tattagattt gatcaagaca gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg
    10801 tgacattacc cacttcagtg ctgtttatcg ccggacacga tactaatctg gcaaatctcg
    10861 gcggcgcact ggagctcaac tggacgcttc ccggtcagcc ggataacacg ccgccaggtg
    10921 gtgaactggt gtttgaacgc tggcgtcggc taagcgataa cagccagtgg attcaggttt
    10981 cgctggtctt ccagacttta cagcagatgc gtgataaaac gccgctgtca ttaaatacgc
    11041 cgcccggaga ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg cagggcatgt
    11101 gttcgttggc aggttttacg caaatcgtga atgaagcacg cataccggcg tgcagtttgt
    11161 aatgcataaa aaagagcatt cagttacctg aatgctctga ggctgatgac aaacgaagaa
    11221 ctgtctaatg cgtagaccgg aaaaggcgtt cacgccgcat ccggccactt tcagttttac
    11281 tctttctcgg agtaactata accgtaatag ttatagccgt aactgtaagc ggtgctggcg
    11341 cgtttaatca caccattgag gatagcgcct ttaatattga cgcctgcctg ttccagacgc
    11401 tgcattgaca aactcacctc tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg
    11461 gtgccaacag aacgccccac gaccgcggca tcactcaccg ccagcatcgg cggcgtatcg
    11521 acaatcacca gatcgtaatg gtcgttcgcc cattccagta attgacgcat ccgatcgcgc
    11581 atcagcagtt cagacgggtt aggtggcacc tgaccgcgag taatcacatc aaagcctcct
    11641 ttgccaaaat gctggatcac tttgttgagc tcatctttac ctgccagata ttccgacaag
    11701 ccatgttcat tactcacggt aaacaggtta tgcgaataac cacggcgtaa gtcggcatca
    11761 ataaataaca ctttttgatc ggactgggcg atcaccgctg ccagagttga actgacaaac
    11821 gttttaccac tgtctggcgt cgcaccggtg atcatcagaa tgttattctc cgtctccatc
    11881 atagcgaaat gcagactggt tcgtagcgca cgtacggctt ccacagcaga atccgccggg
    11941 ttatccaccg ccaggaaggg gatattttta gtacgatggc gctgctgatt agaaaataaa
    12001 tttttcttac gcagacgggt gcgtttatcc agccactcgg acattgggat agtggcataa
    12061 acgctgatgc cgtgctcttc cagttgttcc ggggcttcta caccacgacg caacatcgca
    12121 cgcgccagca cggcacccac agaaataaac aggccaagaa taaaaccaag caccacattc
    12181 aacgcttttt tcggtttcac tggctgcggc tgagtgactg ccgggtcgat aatccgcacg
    12241 ttaccaatgg cactggattt cgaaatactc aactcctgct ggcggttaag taattgcaga
    12301 tataccgcac ggcccgcttc tacgtcacga cttaaacgca acacttcctg ttgggtggaa
    12361 ggcattgccg atacccgctt attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt
    12421 tccagcagcg cacgataagt tgggtgatct ttcttataca gctgggagat ctctgcctcg
    12481 cggaaggtca gctcattgag ttgattatca acgttcacaa tctgctcaag aacggctttg
    12541 gcttccaggt taaggtcaac cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc
    12601 gcctggtcca gctcgctgcg cacttcaggt aactggcgct gtaagaattc aaggctttgt
    12661 gaatcctgcg ccgcctggcg agcgatattc tgttgcaaat agttgttagc gatgctgttc
    12721 agaatacgag taatcaactg gggatcatca ccagtcatgg taagttccag catcccgctt
    12781 tctttactgc gttcgctaac ggtaaaggtt tcctgcaatg cgttaatcgc ttccagttcg
    12841 gtacgctggc tcaggacaaa ctgtgttcct ggtttggcct taatgtccgc gatagtcagc
    12901 gcaacgccat ctttttccag acgctggccg accataccat tgacggtgaa ctcttcacct
    12961 tccagtgtat agtggccgtt ttccccaacc gtgagtgtca gttgctgatc ctgaccattc
    13021 agttgtggaa tatgcatcca gctgatcgcc agctcacctg gtttttcttt ggttaatctc
    13081 gcccagccgc gacccacaat cggaaaatac ttctgctcaa ctatgtcgcg cagattcagt
    13141 tcagcaatgg ttttaccgag aatcatgcgc gattgcagca gttggatctc cggtgcagac
    13201 tcgggcgatg agttagggat catatcgctc aggccgctga gaatggcgtt gccctgtttt
    13261 tgctcgacct ggaccagagt atctgcctga taaattggtg tgcttaacag cgagtaagcg
    13321 acagcgatca gcgtgaataa cgcggtcacg ctgataataa acttacggtg atcccataac
    13381 tcgccgacca gacgaagcag atcgatctca ttttcctgag tgctgcctgg tggcgtattc
    13441 atatttttag ttgtcatcgt attttataaa ctcaaattac cggctgaggc gcttcgccca
    13501 ttcctgactg gcgcgctcca acataccgta gacatgttca aatgcgtcct gacttttacg
    13561 atagggatcc gggatctctt tctgttccag ccattgccca aacagcattg tcttcccgcg
    13621 gacctcggga gcaattgcgg taacctgagc aatatgttcc gactccattg ccagaatcag
    13681 atcgtaattt cgtgccatct cagcagtgag cttacgtccg gcatgtcctt caagagatac
    13741 gccatgatta gcggcaacat ctgccgctgt cgcatctgca gggtgtttta ccagaccatg
    13801 aacacctgcc gatttgactt ttacgcccgg cagacgttta cgcagcaagc gctcgccaat
    13861 gggcgaacgg caaatgttgc cggtacaaac caccaggatt gagttaaatt ttagttgggc
    13921 catgtttgaa tccaccgtga agtctctgtc aggtcatgca ccccggaaat cgtcggtacc
    13981 agcagagaaa tcacacggtt ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg
    14041 taaggttcaa gctggaattc cgttccgagt atcattgccg atgcgtcctt cgcattcagc
    14101 tgataaatat tggcgatttt gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc
    14161 cccgtcgcat cagcgacatc ctgattcata ccctcagcgt tacccagcgc ttccgccagg
    14221 gtcataccgc tgcggtccat tttcagcgtg ctctgtttac ccacttcacc catcacgaag
    14281 actttcagat catcgttgcg cggaataaat aaaatatcgc ccgggtgcag cagtttgttc
    14341 tggcgcaagt cgccacgctg catcagcgca tagaggttca ctttggtctt tacgccattc
    14401 tgggtgagca ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc
    14461 gcatccatga tggtcagagg aatattggta atcggctgct gacccgattt gctgacttcg
    14521 ccggtgacgt acgctttttg cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt
    14581 tcaatgactg aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg
    14641 gccactttca ggcgaccaat ataggggtag aaaatcgcac catcggcgtt aacccagttg
    14701 ccggtatcac tggcgctacg gtattgccct gcgggggtgg ttagttccgg gtgatcccag
    14761 accgtaacca tcaacacatc accaatcccg atacgatatt catagtttgc gatttgctgc
    14821 tccagctccg ggttagcctg cgacataatc ggtttggcgc gcaactgatc aatcaatcct
    14881 ggcgtgacgg gatagatatt gaccattttg tccaggtcat actgattgtc aggtagttca
    14941 atgacatcct tgttactggt cgataaattc tgaccaggaa caagggtaca tgctgtcaaa
    15001 gacagaacag ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat tggtttatat
    15061 ttatcagtca atgtaatgtt atgaattacg tcaggttatt gtccaaccgg tacttctcgc
    15121 tctgccgtca ttggatagag ctggtatttg cgtgacagca tttgtccacc atcacgcgtc
    15181 aatggtgtcc acgaaaccac cgcgcggttg cggttaggtc caatggtcat caagtcgaat
    15241 ggaatcgaga tataaaaacc tttactaaag ccgccttcgc cgtagtcatc tttagatacg
    15301 ttactgattg ccgcccatac ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg
    15361 atcgttgcgc ctttatcttt tgccagatat tgcccaacgc taagtttcat cagtacgcca
    15421 ttgagcgtcg gcgggttcca gtaagccgtc acgaaacctg ttggcgtgga ataatcggtg
    15481 aaacgcatca tgttgtccca gttacgctgc ttcacgtagt taacgtccac acccagcgcc
    15541 cagctggcat ctagcgggcg ataaagcagc tcggaaccga caccggcata catcgtttcc
    15601 agataaccgc catacacctg gccatagaaa ccattgccta agtcagcaaa atagttcgcc
    15661 tgcaagttgt tgagataaac gtcattgcga acgtagtcac ggatatgcgt gcgcacgcgg
    15721 ggcagggtag agtccgcggg caacagcgaa gacttaaact tgtcgtagtt gttgtaagta
    15781 ttggtgaaaa taccgccatt aagcagcagg tggtcggtaa accagtagcg ggcactggac
    15841 atcagcccca gctggaacat atagaaatct tccgggccgc ccagcgactg tgaaagtgtt
    15901 gggttgaaag agtagctaaa gcgatcttca cgaatacggt agccccgacc aaaggcagaa
    15961 agatcttctg cttcaacacg ttgttgttgc agttgctctg atttaccagg cgctgtacca
    16021 gccagctgtt tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc catatgctcg
    16081 cgcttttgcg tcacgctaat cttctcaacg ccttgcggca ggttattcac cagaatccgg
    16141 ttggcacgat cgaccgcttc gcgagaatca cggtatttgt attgttgacc agacatatac
    16201 agcgtcttat cgcgcagctg aatttccggt gcttcaaaac ctgcgttgta cttcagcgcg
    16261 gtaagttggt tcgctactgt ggtgtactgc aatccttcag attcaggcgc aggttgatat
    16321 gccggttttg gcgtatcgcg cagggcaggg cgcagatcgt tgaaattggt ccgtaacgtg
    16381 aagccaaaca tcaacgtgtt accgcgttca taactcaggt tgagatctgc ccagctggca
    16441 gcgcgataaa ctgcgccgac gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca
    16501 ttctggtaat tgttgccatc gtattcgagt ttcagacgca ggggattcca cggcgtttga
    16561 tactcaatgc cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg
    16621 cccgcatcgt gagactctgc gcgatgacaa tatttatcgc tcacccggca aaacgggttg
    16681 gtaatattgc ccgcattacc ggcgtagccc catgccatcc cgagggtgaa atcaaatggc
    16741 cccgcttgct tgctggccac cagatactca ccgtcaaaca gacccgtacc agcaatatca
    16801 cgtttaccaa acgccacttg cggtagccag taaccttctt cccacaggcg aagtttaaaa
    16861 tcgaatgatt tgtctttgta tgactgatcg ccgctgaaat cttcccactg gctatatttg
    16921 cgtgtgcgca catccgtata acgaatggtg ccttccagcc atgggaacag cgccacggag
    16981 gtggagtaga accggtattg atcgttatcc cggtagttga cgctgaattc accttccggt
    17041 gcgatgcgcg catttggcat ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa
    17101 cccagcggat ccgggtaggt taatacttca gcatgacagg cactggagac ggcaatggcc
    17161 aggcagctta aaagataaga attcttcttc atcagtcagg aacacgttgc gttaaaacag
    17221 acacaatctg gtcatttaaa tcagcatatt tttcgggcag tacatgcgca gaaaagccca
    17281 accaaagctg gctgccaggc ggtggttcca catgtctttt attccacagg gcaacagggg
    17341 cgacgacagc ctcaccctca ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa
    17401 ggcgggggtg atcttgcaga taatcggtaa cgctacggcc tgcctgccag ggcaattgcc
    17461 cggccccaga aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg gtatagagcg
    17521 tatagtcccc gaccaacggg ggattgctgt tctcatccac tcgtacaaaa tcagggtcca
    17581 gtttgaccgg taagcgacca gtgatattga gattgagtaa ctgctggcgc acggatttaa
    17641 tcgttgccgc cacatcgtcg tcagcttctg cttcccagga agcaagctgt gccatgacat
    17701 gttggtagtc tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc
    17761 accagaggcg atcgcgcagt tgcggttgtg tcaccaactg gacaacattt tccacggggc
    17821 caacggaaag tgtctgttgt tcgccaggca gataaatagt caccgttcct tgcgcaaagg
    17881 catggggtgt cattacgtaa agtacgctgg caatgaaata cgactgtaat ttattcatgg
    17941 tgcgggttta agaaacgtca tttctacggg aataacccct gcgcctaaca tttgccgact
    18001 ttgacgaacc tggccagtag ccgaatcgac ccagaatgtg ttttgccact gtttttctgg
    18061 ccgcgtggaa gtcacctctt cctgccagac attacacagc acattgcgac ccgcaatatt
    18121 cagcgtctct aatccagcaa aggaaaaagt agaactcagg gtcgcagagc gaaaatggtt
    18181 gtcttcactc cagagaatat cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt
    18241 acgcagaggg tcctgcccgg aatttgtcac ttccagcaga ttattattaa gtttgacggt
    18301 ttttaaaaga cgtccattgt gggtaaccag catggcgtta tcctgggata accatttgct
    18361 ttgttcttgt tctatatatc ccagtaccac aaagattcgt tgcccttcat taaggcgtaa
    18421 atacatcgtg gaataaggca acgcctggat ctgctgatcc gctacggtga tatcctgatt
    18481 atcgaaaagg ctggcgcgac atgtatcgac catactttgc tggctgtgcg tacatcccgc
    18541 aagaaatagt gcgaaaatcg ataaaataag agggcgcacc tttaatcctt gaaaggttaa
    18601 ttaaaaaaat gcaaatctct aaagcccggt acgaatatcg caaccgggta tcatttaact
    18661 ttaatttcga cgcgttaaag cagtattagc gtgtagtgga cgtggtggtt gaggtgttat
    18721 gagaaccgcc atcgccactg tcatttgctg caacaactgc caacgtcgcc acaccaacag
    18781 ccgttgccac accgacaact gtcgccgtgc cgacagcgcc agcagccacg ccaccagtgg
    18841 tagtagcagt cgtggttgcg ccggtagtgg tcgttgtggt gccagtagta cccgtcgttg
    18901 cctcggtccc gtttgttgct tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg
    18961 ccataaggat tgcagaaagc ttgtgtttca taacttttcc tttattcatc gcatggacaa
    19021 tacgtacgct tgagatcagg attgtctact ttgagtataa catccttgtg aaatctccct
    19081 ttcctcatac cgcttgcact atatactgcg ggtaggaaag gcgcgcagag ggaaatataa
    19141 gattgttaac tatccccctc tggaaatacg acttgt
//
LOCUS       DAZFAH010000076        19120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFAH010000076 DAZFAH010000000
VERSION     DAZFAH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.076"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..596
                     /gene="yfiR"
                     /locus_tag="ACHJWB_002526"
     CDS             78..596
                     /gene="yfiR"
                     /locus_tag="ACHJWB_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4314142.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            586..1812
                     /gene="dgcN"
                     /locus_tag="ACHJWB_002527"
     CDS             586..1812
                     /gene="dgcN"
                     /locus_tag="ACHJWB_002527"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4314143.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1828..2310
                     /gene="yfiB"
                     /locus_tag="ACHJWB_002528"
     CDS             1828..2310
                     /gene="yfiB"
                     /locus_tag="ACHJWB_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4314144.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2387..2734)
                     /gene="rplS"
                     /locus_tag="ACHJWB_002529"
     CDS             complement(2387..2734)
                     /gene="rplS"
                     /locus_tag="ACHJWB_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4314145.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2776..3543)
                     /gene="trmD"
                     /locus_tag="ACHJWB_002530"
     CDS             complement(2776..3543)
                     /gene="trmD"
                     /locus_tag="ACHJWB_002530"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4314146.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3574..4122)
                     /gene="rimM"
                     /locus_tag="ACHJWB_002531"
     CDS             complement(3574..4122)
                     /gene="rimM"
                     /locus_tag="ACHJWB_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4314147.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4141..4389)
                     /gene="rpsP"
                     /locus_tag="ACHJWB_002532"
     CDS             complement(4141..4389)
                     /gene="rpsP"
                     /locus_tag="ACHJWB_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4314148.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4638..5999)
                     /gene="ffh"
                     /locus_tag="ACHJWB_002533"
     CDS             complement(4638..5999)
                     /gene="ffh"
                     /locus_tag="ACHJWB_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4314149.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMAGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6166..6957
                     /gene="ypjD"
                     /locus_tag="ACHJWB_002534"
     CDS             6166..6957
                     /gene="ypjD"
                     /locus_tag="ACHJWB_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4314150.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6978..8264
                     /gene="yfjD"
                     /locus_tag="ACHJWB_002535"
     CDS             6978..8264
                     /gene="yfjD"
                     /locus_tag="ACHJWB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4314151.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGSANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            8387..8992
                     /locus_tag="ACHJWB_002536"
     CDS             8387..8992
                     /locus_tag="ACHJWB_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO4314152.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(9027..9617)
                     /gene="grpE"
                     /locus_tag="ACHJWB_002537"
     CDS             complement(9027..9617)
                     /gene="grpE"
                     /locus_tag="ACHJWB_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4314153.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPCDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            9739..10617
                     /gene="nadK"
                     /locus_tag="ACHJWB_002538"
     CDS             9739..10617
                     /gene="nadK"
                     /locus_tag="ACHJWB_002538"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4314154.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10703..12364
                     /gene="recN"
                     /locus_tag="ACHJWB_002539"
     CDS             10703..12364
                     /gene="recN"
                     /locus_tag="ACHJWB_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4314155.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGATRADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYTNETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12513..12854
                     /gene="bamE"
                     /locus_tag="ACHJWB_002540"
     CDS             12513..12854
                     /gene="bamE"
                     /locus_tag="ACHJWB_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4314156.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYSLGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12916..13206)
                     /gene="yfjF"
                     /locus_tag="ACHJWB_002541"
     CDS             complement(12916..13206)
                     /gene="yfjF"
                     /locus_tag="ACHJWB_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4314157.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(13196..13633)
                     /gene="ratA"
                     /locus_tag="ACHJWB_002542"
     CDS             complement(13196..13633)
                     /gene="ratA"
                     /locus_tag="ACHJWB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4314158.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13804..14286
                     /gene="smpB"
                     /locus_tag="ACHJWB_002543"
     CDS             13804..14286
                     /gene="smpB"
                     /locus_tag="ACHJWB_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4314159.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14501..14863
                     /gene="ssrA"
                     /locus_tag="ACHJWB_002544"
     tmRNA           14501..14863
                     /gene="ssrA"
                     /locus_tag="ACHJWB_002544"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(15024..18279)
                     /locus_tag="ACHJWB_002545"
                     /pseudo
     CDS             complement(15024..18279)
                     /locus_tag="ACHJWB_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(18344..18463)
                     /locus_tag="ACHJWB_002546"
     CDS             complement(18344..18463)
                     /locus_tag="ACHJWB_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4314160.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(18687..18845)
                     /locus_tag="ACHJWB_002547"
                     /pseudo
     CDS             complement(18687..18845)
                     /locus_tag="ACHJWB_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         4784 a         5030 c         4401 g         4905 t
ORIGIN      
        1 catgaaaaaa tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag
       61 atggtgggga tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc
      121 tgacgggagc accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga
      181 tggtttccgg tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat
      241 gtatcttttc atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc
      301 cttatctgcc tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt
      361 tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa
      421 aggctttatt attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc
      481 tgatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg
      541 gtgtaaaggt caacccagat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat
      601 aattctctta ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc
      661 atatttatca ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg
      721 aaacagtacg cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa
      781 gcagctgtcg tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag
      841 caagggcaat tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg
      901 cattacaccc gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc
      961 cctgccccca tcattcagcc gattcgtcac aatggcgaaa ccattggcga agtacgctta
     1021 accgctcgcg acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt
     1081 tgtattctgc tggcatcagg aatcgcaatt accctcaccc gccatttgca caatggcctg
     1141 gtggaagcac tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt
     1201 tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     1261 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggctaaaaa tgcgcagctt
     1321 ctacgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     1381 ggcatcaaca cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt
     1441 gatggcgata atttcaaata catcaatgat acctggggtc atgcgacggg cgatagagtc
     1501 ttgattgaaa tcgcaaaacg gttagctgaa gttggcgggc tgcgacataa agcataccgc
     1561 ctgggcggcg atgaattcgc tatggtgctc tatgatgtac agtcagaatc tgaagtgcag
     1621 cagatatgct cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat
     1681 cagaccacca tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa
     1741 aaattacaag agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag
     1801 ctggtgagat aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc
     1861 acctcactaa tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc
     1921 gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca
     1981 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc
     2041 caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac
     2101 accgataact atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc
     2161 gtggccgatg catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt
     2221 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc
     2281 cgcgtcgcag tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca
     2341 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct
     2401 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     2461 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     2521 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     2581 tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     2641 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     2701 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     2761 tgatatctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     2821 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     2881 aggtcttcta agccaagtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     2941 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt
     3001 atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg
     3061 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     3121 accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac
     3181 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     3241 ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     3301 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     3361 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt
     3421 gaagtcgcga ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt
     3481 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca
     3541 catagcgccg tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc
     3601 gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag
     3661 acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga
     3721 tccggtttcc atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg
     3781 gcagcccatc aggtctttcc agtagtagtc cccctcttca agctgaggca gctgcgatga
     3841 atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt
     3901 cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca
     3961 ctgacccgcc ttctggataa accaaggctg atagtcaaaa atgctttcgg cgtcttcggt
     4021 ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac
     4081 gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga
     4141 ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc
     4201 ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct
     4261 agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct
     4321 gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat
     4381 agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc
     4441 cggtgtcata ttaaaagccc gaaaatttta ctcatttttg cgggaattgc aatcaacagt
     4501 tgctaactct gctgtaaaag gccgtcggcg gtgcagccag tttggtgccg gagtgcgcgc
     4561 agtcaccgga gcgtacacgc agtacgtgag gatgacgagc acatcccggt gccaaaatgg
     4621 caaacaagcc aggccgatta gcgaccaggg aagcctgggg gcatcatacc cttcatgctt
     4681 ctcatcatct tcgccattcc gcctttcttc attttcttca tcatgcgctg catgtcgtcg
     4741 aactgcttca gaaggcggtt cacgtcctgc acctgcatac cgcaaccggc agcgatacga
     4801 cgcttacgcg aacctttgat gatttctggc ttagcgcgct ctttcatcgt catcgagttg
     4861 atgatggctt ccatacgcac cagcacttta tcgtccatct gcgacttaac attatccggg
     4921 atctgcccca tgcccggcag cttgcccatc aggctagcca tgccgcccat atttttcatc
     4981 tggcgcagtt gctcaagaaa atcgttgaga tcgaagccgt cgcctttttt cagcttgctg
     5041 gctaatttct ctgcctgcgc gcggtcaact ttgctttcga tatcttcgat cagcgacagt
     5101 acgtcgccca tgccgagaat acgcgacgcg atgcggtccg gatggaacgg ctccagcgcc
     5161 tcagttttct cgccaacacc gagaaacttg atcggtttgc cagtgatgtg acgaatagag
     5221 agcgccgcac cgccgcgggc atcgccgtcc actttggtca acactacgcc ggtaagcggt
     5281 aacgcttcat tgaatgcttt tgccgtattg gccgcatcct gaccggccat ggcgtcaacc
     5341 acaaacaggg tttcaaccgg gttaatcgac gcatggactt gtttgatctc gtccatcatc
     5401 gcttcgtcaa cgtgcagacg accagcggta tccaccagca gcacgtcgta gaatttcagt
     5461 ttggcttctt tcagcgccgc gttaacgata tctaccggct tctgaccaac atcagaaggg
     5521 aagaaatcaa cgcccacctg ctctgccagc gtctcaagct gtttgattgc cgccgggcga
     5581 taaacgtcgg cagaaaccac cagcactttc ttcttgtgct tctcgcgcag gaacttaccg
     5641 agtttaccaa cgctggttgt tttaccggca ccttgcaggc ccgccatcag cacgaccgca
     5701 ggcggttgcg ccgccaggtt cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg
     5761 ttacggacga ttttgacgaa ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca
     5821 accgctttct cttttacgcg attgataaac tcacgcacta ccggcagagc gacgtccgcc
     5881 tccagcagcg ccatgcgcac ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt
     5941 ccacggccac tgatattgcg cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt
     6001 gtctctcgcc tggggtggaa acggttggcc gcaatcgcga cacatcatca gtattttgcc
     6061 gcagtataac atgaaggcgt ctttgttgtt atgcaacggt tggagcagcg ttcacctgac
     6121 gctatactgc ttctctttct tattgctcaa actgtcgaca tcactatgcc cgtttttgct
     6181 ctgctcgcgc ttgtcgccta ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa
     6241 aaaaacggcg gctggcggcg catggctatt atttctgcgg tcattgcgct ggtctgccac
     6301 gcaatcgctc tggaagcccg catcctgccc gacggtgata gcggacaaaa cctcagcctg
     6361 ctgaacgttg gttcattggt cagtttgatg atctgtacgg taatgaccat tgtggcttct
     6421 cgcaatcgtg gctggttgct gctacccatt gtctatgcct ttgcgcttat caacctggcg
     6481 ctggcgacct tcatgcccaa tgaatacatc acccatctgg aagcaacgcc tgggatgctg
     6541 gtgcacattg gcttatcgct cttttcctat gccacgctaa ttatcgccgc cctgtacgcg
     6601 ctgcaactgg cgtggattga ttaccaactg aagaacaaga agctggcatt taaccaggaa
     6661 atgccgccat tgatgagtat cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg
     6721 ctgctaacgc tcacgctttg cactggcctg ttctacatgc acaacctatt tagcatggaa
     6781 aatatcgaca aggctgtgct ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg
     6841 ggacattacc atgaaggatg gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggcgcg
     6901 gtaattctga cactggccta cttcggcagc cgaattgtcc agcagttaat cagctaaacc
     6961 cagaaaagga gtttcccctg gaacacattt ctactactac gttgatcatt attctgatca
     7021 tcatggtggt catttcagcc tatttttccg ggtccgaaac cggaatgatg accctcaacc
     7081 gctatcgtct gcgacatatg gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat
     7141 tgctgcgtaa gccagaccgc ctgataagcc tggtgttaat cggcaataac ctggtcaata
     7201 ttcttgcctc cgcgctcggc actattgttg ggatgcgttt gtacggcgat gcgggcgtgg
     7261 caattgcgac tggtgtgctg acttttgtcg tactggtatt tgctgaggta ttgccgaaaa
     7321 ccattgccgc gctgtacccg gaaaaagtcg cttatccgag tagttttctg ctggctccgc
     7381 tgcaaatttt gatgatgccg ctggtctggt tgctgaatgc tatcacccgt atgctgatgc
     7441 gcatgatggg tatcaaaacc gatatcgtgg ttagcggctc tttgagcaaa gaagagttgc
     7501 gcactatcgt gcacgaatca cgctcacaaa tttcccgtcg caatcaggat atgctgctgt
     7561 cggtgctcga tctggaaaaa atgaccgttg atgacatcat ggtgccgcgc agtgaaatta
     7621 tcggtattga tatcaacgat gactggaaat cgattctgcg ccaactctcc cactcacctc
     7681 acgggcgcat cgtgctctac cgtgattcgc tggacgacgc catcagtatg ctgcgagtac
     7741 gtgaagcctg gcggttaatg tcggagaaaa aagagttcac caaagaaacc atgctgcgcg
     7801 ccgcggacga gatctatttt gtgcccgaag gtacgccgct cagcacgcag ttggtaaagt
     7861 ttcagcgcaa caaaaagaaa gtcggcctgg tcgtcaacga gtatggagac attcaggggc
     7921 tggtgacggt tgaagatatt ctggaagaga ttgtcggcga tttcaccacg tcgatgtcgc
     7981 caacacttgc cgaagaggtc acgccgcaaa acgacggttc ggtgattatt gatggctctg
     8041 ccaacgtgcg agaaatcaac aaagccttta actggcatct accggaagat gatgctcgta
     8101 ctgttaacgg tgtcattctg gaagcgctgg aggagatccc ggtagcaggc acccgcgtgc
     8161 gtattggcga gtacgatatc gatattctcg acgtacagga caatatgatt aagcaggtaa
     8221 aagtttttcc tgtgaaaccg ctgcgcgaga gtgtggcgga gtaacgcaat ggcgaactcc
     8281 ggttcgcctt ttttttatcg ccgtctttta ggattccatc aattcctgtc atttacttag
     8341 caatgtaata atgtcttttc aagcattcgg aaaggatatt gatgttatgt cagaagatta
     8401 tgtaattgaa tgggataagg attttgcaga cgatctcaac gtcgtcgcca gcgttttttt
     8461 acagcacacc ccaaccttat ggcccactat tttctctcaa ctttcgacgc aacctgaaat
     8521 ctttgaagac gaagatgaag acgaatatgg attgcaggac gttctcgatt gcagtggtgg
     8581 cgatctcggg aatcgtgacc tggcgcaggc atttttacag gttctacgcg gtgaaggatt
     8641 aattcaactc gttgactgga aaggtgaaga tgaagaaggt gagctggcta attttgctgc
     8701 agatcgcttt tatgagctca caaaaaacct gatagcttct gaggaattaa ggagtcttct
     8761 tgtcgaaata acccaagagg atgaaatctc cgatgtctgt gaagccggag accgctacct
     8821 tgacgaaatc tttgaacgca tccaaaccga actcaataaa aggggctttc agatttttga
     8881 tctgaatgaa ggatctgaca cctataacgt ggtcgttctg ccaatgaacg aatataaaaa
     8941 aatagatgat ttcaacacgc cgtggctgga agtacaggat tttttaagct aaaaaaatgg
     9001 ccgacttcgc gtcggccatc agatatttac ttcgccttcg ctacagtaac catcgccgca
     9061 cgaatcgtac gaccattcag cgtataaccc ttctgcataa tgccgagtac gttacctggc
     9121 gcaacgtcat cagattccac cattgcgatg gcctgatgca cattcgggtc cagtgggacg
     9181 ttagtttcgg cgatcacttc aacgccaaac ttacgcacaa catccagcat cgacttcagc
     9241 gtcagctcaa tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc
     9301 agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg
     9361 aatttgtggg ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct
     9421 tttacacgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga
     9481 ttcgcaattt tttcatcgca cggatccacc tgctcagcag aagcttctgg ctcaactgcc
     9541 tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc
     9601 tgttctttac tactcatgaa tttctccgcg ttttttcgca ttcatctcgc taacttcgct
     9661 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
     9721 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
     9781 cggcacccta ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
     9841 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
     9901 ggcacgctcg cggagattgg gcaactggct gatctcgcgg ttgtcgttgg cggcgacggt
     9961 aatatgctgg gcgcggcgcg cacgctcgcc cgctacgata ttaaagttat tggaatcaac
    10021 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acaattagcc
    10081 gatgtgctgg aaggtcacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
    10141 cagcaagatt gccagaaacg catcagcacc gcgataaatg aagtggtact tcatccaggc
    10201 aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttctcag
    10261 cgttccgatg gactaattat ttcgacgcct acaggctcca ccgcctattc cctctctgca
    10321 ggcggtccta ttctgacacc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    10381 acgttgtcag cacgaccact ggtgataaac agcagcagca cgatccgtct gcgtttttcg
    10441 catcgccgta acgacctgga aatcagttgc gacagccaga tagcactacc tattcaggaa
    10501 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    10561 tacagttatt tcaatacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    10621 tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg
    10681 tttttcatac aggaaaacga ctatgttggc acaactgacc atcagcaact ttgctatcgt
    10741 tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggcgc
    10801 gggtaaatct attgcaatag atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga
    10861 catggtgcgt accggcgcta ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac
    10921 gccagcggcc ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct
    10981 tcgtcgcgtg atcagcagtg atggtcgctc ccgtggtttc atcaacggta cagctgttcc
    11041 tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc agcacgctca
    11101 tcagttactc accaaacccg agcaccaaaa attcctgctt gatggctata ccaatgaaac
    11161 ctctctgctc caggaaatga ccgcacgtta tcagttgtgg catcaaagct gccgtgacct
    11221 cgcgcatcat caacagttaa gtcaggaacg cgctgcccgt gcggaactgt tgcaatacca
    11281 attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgagcaaa tcgacgaaga
    11341 gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt
    11401 aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt
    11461 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc
    11521 taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc gtctggatct
    11581 cgatcccaac cgactgtttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
    11641 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
    11701 gcagcaacta gacgatcagg ccgactcaca agaaacgctc gcgctggcgg taacgaaaca
    11761 tcatcagcag gcactggaaa ccgcgcgcgc attacaccaa caacgccagc aatatgcaga
    11821 agaacttgca cagctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt
    11881 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggtgccg atcgtattga
    11941 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
    12001 tggtgaattg tcccgcatcg cactggcaat ccaggtcatc acggcgcgta aaatggaaac
    12061 cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt
    12121 cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc
    12181 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
    12241 gacagaaacg catatgcaat ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct
    12301 gcttggtggc agtgaagtca cacgtaatac actggcgaat gcgaaagaac tgcttgcggc
    12361 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
    12421 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
    12481 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
    12541 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
    12601 ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca
    12661 tgacgcaaca acaagttgcg tactcattgg gtacaccgct gatgtccgat ccatttggta
    12721 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
    12781 cgctgacgct gacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc
    12841 tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
    12901 ccttttttct gtccgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt
    12961 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
    13021 tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg
    13081 caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt
    13141 cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
    13201 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gccgccagct
    13261 ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca aagtcgagat
    13321 gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt
    13381 tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc aactggttgc
    13441 gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc
    13501 caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga ggataagact
    13561 gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc
    13621 taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg
    13681 ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct
    13741 tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg
    13801 cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc
    13861 gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg
    13921 gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt
    13981 gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc ctccacgcat
    14041 gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga actggactca
    14101 ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa
    14161 aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca cgataaacgt
    14221 tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa aaacgcccac
    14281 cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc tccggcggcg
    14341 cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc attgcttaat
    14401 cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt
    14461 catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt ctggattcga
    14521 cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa
    14581 aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct
    14641 ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg
    14701 cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc
    14761 gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat
    14821 gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga ttggacggta
    14881 taaggacaac accaataaaa acaggaagtt agcagtctca gcaggacacc gaccagacgg
    14941 tgaagagaca taaaaggata cgcaaaggag ccgcggctcc tggtgacatg aaagcccaca
    15001 gatgtgggct ttttcgttga tggtcagaac gaccagttca caccagctac cccgttccac
    15061 ggggattcca caccggcacc atggctatac cccaccccaa gatgcccgct taacgtactg
    15121 ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgacg
    15181 aaattaccgt cactattcac tttcacccgg ttatcatcga caaattcttt gcgcacagcc
    15241 gccttcagcc acggctcaac ttccataccg ttccccagac gcatgttgta actcagcgtt
    15301 gcacccagtt cacgatataa actgcgggta tcgactgatt tcgattccat gccattggat
    15361 aaatgatatt cagggttatc agcggtgaac ccccgttaac gatgcatacg gcgtcaggtt
    15421 ccagttacca tcggtaaatc gcatcccggt ttcaatgtga ccgcccagcc cgttgctgcg
    15481 ataactgccg ttggcggctc caccgctgcc aaccgtggag tcgcgagctt cggtacctgc
    15541 taataccaat gaattaccgc cattttccag caacatattg gtcgctaaat tgccggaaat
    15601 ggaaaaagtg ccgtactggt gagtaccgct gatttcaata ccgttagccg tgctcgtctg
    15661 gagagcggca ccgctgttct ggacgatatc tgtcgctttg ccattatcgt taactgtcag
    15721 cgtaccgcct tcattgatct ttgtttttat tgcctctccg ttagctgaaa ctgtttgtat
    15781 tccgccgtcg ttaatcgttg tctcattcgc caccccctcg acgatttgtt cgccgccggt
    15841 gagcgtcgtg cctgtcgcag tggcttttgt tttgacgatc tcccgtccgc ccgtattgac
    15901 ctgtgttttg tcagaagagg tgtctgactc cacggttaac acgccgccat ttgccagcag
    15961 gatattgttc gccgcaccct gctcgatgct gaacgcaacg ccatccgcgc gtgttcctgt
    16021 gacccgcgtc gccctggtgg ttgcaaccaa agcgccctgg ctactctgct gtatccccgt
    16081 tgcgctgcct ttctcccgca catcgagtgt accgccgtca ttaagcaccg agttttcagc
    16141 cagaccgccc tcattaacta cctgtgaacc cccattaata atggaacctt ccgctatccc
    16201 gtttgccata atttgttgta ggccagagac gatatcggta ttgatcgcct taccataatt
    16261 ctgaacggtt tgtgtgccgc cattgatgtg cgttttgtca gttgacccac catcaacaat
    16321 ttgttcacca ctttcaatat ttgcctcagt ggctaaacca tataccgttt gcttgccacc
    16381 tttgatattt gctttatcag aagtggcact ggcatatatt gtttgggtgc cagcactatt
    16441 aagtacagtg ccaacatctt ttccataaac atccattttg ccgttggcat taataatcgt
    16501 atcaactgcc cgggaaccag tgacgactgt taatgagcca gcgttttcca gcactacatt
    16561 tttagcttct gaattcctga tgtagaaagc atcaccataa ctgttggttc cttcgataag
    16621 ggttccggaa gttgtggaag caattaatgc gccgccggat tgttgctcaa catgcttagc
    16681 ctcaccaccg tcctgaacct ccagaacgcc accattatta agtctggtgg tatctgtttt
    16741 agcctccttc tggacaatca gcttaccgcc agcatcaacg gtagtatttt tcgccgaggt
    16801 tttagccact accgtcagtt cgccggtatt ttccagcaca acataattag cctcccctcc
    16861 ggtaatagtg aagtgagaga gcttgttgta tccttcaata tcagtccctg cgcccgtgtt
    16921 ggcaactaaa gcactgcccg tctcctggtt aaccccatgt gcaataccgc cggtatagac
    16981 aatcagcgag cctccggcgc taatattgct gccaattgcc gtactatctt tctcaacaac
    17041 ttgctggctc ccggaggata taattgttgt gtcagctttc ccaccgcttt tgatattttg
    17101 cgttccgctg ttgatattgg tgcctgtggc tatgccataa ttattaagat tctgtgtgcc
    17161 accattaatt atggtatttg tcgcgtttcc tgcaacatcc ataaccccgc cattatctat
    17221 tcgggtcgca tcagctttag cattcgttaa aactgacatt gttcctttat ctttaataat
    17281 cgtcttgttt gcggaatgag atacttctaa atggcctcca ttttccagca acacattgtc
    17341 ttccacttta ttgtggatgg agaatgcacc ttcactattc gtaccgctca ccgtcgtacc
    17401 gttagtgttt gtttttaaaa ttgcaccatc gtgctgggta acatttgttg ccgtaccacc
    17461 actaacatca agcacgccac cggaataaac ttcaataaca tccgaggtgc tggtgttatc
    17521 aacaatttgc gtgccaccag aatagatctg cgtaattttt gccgttgact tactattcag
    17581 agactgagtt ccgccttcaa tcgtcgtgtc cagcgcacgg ctctcatata ctctttgctc
    17641 accgccattt ttaatggttg ttgtttctac tgtgctctgt tcaacatact gtcgaccacc
    17701 atttatggtt gtgttcgttg ccagacttcc ttgtactacg tcctgagagc cagacttatt
    17761 tatcgttgta ccatcagcat gcccttgaac ttttactatc tggctaccac catcaatgag
    17821 tattccattc gcactccctc cctctacgcg tgaagcaccg cccttaattg tcgttccatt
    17881 gctgataccc cctttataaa cgtcctgatt gccactctcg attgtcgtac ctgtggaaat
    17941 acccccgtca tgaattgact gtctgccacc gttaatggtt gtattattag cctgccccac
    18001 aaaattgtta tgacttccta tatcttgata tccaccagat tcaataagac ttccattaga
    18061 taccccgcca tgaacattct gctggccatg gttgataata tgagtgttat ttgttgtacc
    18121 tcgttcatcc actttttggt tgccatctac agtctcatcg tttaccacac caataacatc
    18181 aggagtgaag gccgccatac cgggcggggc atatatcaag gcagatatca ataaggaaag
    18241 tactgagcgg cgacaataat ggggactggt cctgttcata aatttcatcc tctgaaaagt
    18301 gaatactgag tagcgtttaa gcgaccttag ctctgctgca acatcagccc acaggcacca
    18361 gaccagggga ttcatcctga agagacagcg caagtgtatt gtgttcaccg ctcatcaaag
    18421 acatcatgat gaaatgatga tattccacat aagaaagaag cattttttaa acgcagtgcg
    18481 ctgaagtatg gttggataaa aaagtcaatc tattcaggga atacgggggt attcttttct
    18541 ttcgacaatc aggccgtcgg caaaataaaa tgatttacat aatcgtttct gatgaatatc
    18601 ttctgctcac ataaaaatca cacaataact ttgagatcgc agattctttt acttttacag
    18661 cattcgtccc cccattgttg ggcaaatata gattgggcca gagcacgaaa gttaatacca
    18721 cgtttgcaca gctcctccaa cagcacgaca agatgccaca tactgcgccc cagtcggttc
    18781 ggtttacaga ctacccgtgc gtccaccgcc gataatgtcc tgagcagttt tttcagtccg
    18841 gacctcctgc cacggtttta cgttgaagag gtaaccctga gcacgcagtt catcagtcag
    18901 gcgtggtgta ccgtaaccct attattgatg ggtaagatca aaaaaacttt caggcagcta
    18961 aggaaagttg aaccagacat tagaataaat atttcaacca attacagcac caattcagac
    19021 accgccagct caacaaataa atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
    19081 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg
//
LOCUS       DAZFAH010000077        19071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFAH010000077 DAZFAH010000000
VERSION     DAZFAH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.077"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..2331)
                     /locus_tag="ACHJWB_002548"
     CDS             complement(43..2331)
                     /locus_tag="ACHJWB_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4314161.1"
                     /translation="MAVKASGRFVPSSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEEICDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTTPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHDLRTPNRQ
                     QRSGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWEIEALARGATVNYDG
                     KKFTYMVINEWFGFRDEESFFM"
     gene            complement(2321..2596)
                     /locus_tag="ACHJWB_002549"
     CDS             complement(2321..2596)
                     /locus_tag="ACHJWB_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO4314162.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGTHGR"
     gene            complement(2593..2817)
                     /locus_tag="ACHJWB_002550"
     CDS             complement(2593..2817)
                     /locus_tag="ACHJWB_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFO4314163.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(2817..3119)
                     /locus_tag="ACHJWB_002551"
     CDS             complement(2817..3119)
                     /locus_tag="ACHJWB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO4314164.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLGLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADEFKKLNDKRNW"
     gene            complement(3119..3343)
                     /locus_tag="ACHJWB_002552"
     CDS             complement(3119..3343)
                     /locus_tag="ACHJWB_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO4314165.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3407..3907)
                     /locus_tag="ACHJWB_002553"
     CDS             complement(3407..3907)
                     /locus_tag="ACHJWB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO4314166.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4077..4349)
                     /locus_tag="ACHJWB_002554"
     CDS             complement(4077..4349)
                     /locus_tag="ACHJWB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO4314167.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            4486..4779
                     /locus_tag="ACHJWB_002555"
     CDS             4486..4779
                     /locus_tag="ACHJWB_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4314168.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            4849..5829
                     /locus_tag="ACHJWB_002556"
     CDS             4849..5829
                     /locus_tag="ACHJWB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4314169.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6016..6516)
                     /gene="cpxP"
                     /locus_tag="ACHJWB_002557"
     CDS             complement(6016..6516)
                     /gene="cpxP"
                     /locus_tag="ACHJWB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4314170.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            6666..7364
                     /gene="cpxR"
                     /locus_tag="ACHJWB_002558"
     CDS             6666..7364
                     /gene="cpxR"
                     /locus_tag="ACHJWB_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4314171.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7361..8734
                     /gene="cpxA"
                     /locus_tag="ACHJWB_002559"
     CDS             7361..8734
                     /gene="cpxA"
                     /locus_tag="ACHJWB_002559"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4314172.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(8840..9514)
                     /gene="yiiM"
                     /locus_tag="ACHJWB_002560"
     CDS             complement(8840..9514)
                     /gene="yiiM"
                     /locus_tag="ACHJWB_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4314173.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(9663..10646)
                     /gene="kdgT"
                     /locus_tag="ACHJWB_002561"
     CDS             complement(9663..10646)
                     /gene="kdgT"
                     /locus_tag="ACHJWB_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4314174.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLEILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(10906..11526)
                     /gene="sodA"
                     /locus_tag="ACHJWB_002562"
     CDS             complement(10906..11526)
                     /gene="sodA"
                     /locus_tag="ACHJWB_002562"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4314175.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            11811..12845
                     /gene="rhaT"
                     /locus_tag="ACHJWB_002563"
     CDS             11811..12845
                     /gene="rhaT"
                     /locus_tag="ACHJWB_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO4314176.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(12842..13690)
                     /gene="rhaR"
                     /locus_tag="ACHJWB_002564"
     CDS             complement(12842..13690)
                     /gene="rhaR"
                     /locus_tag="ACHJWB_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4314177.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(13764..14600)
                     /gene="rhaS"
                     /locus_tag="ACHJWB_002565"
     CDS             complement(13764..14600)
                     /gene="rhaS"
                     /locus_tag="ACHJWB_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO4314178.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            14888..16357
                     /gene="rhaB"
                     /locus_tag="ACHJWB_002566"
     CDS             14888..16357
                     /gene="rhaB"
                     /locus_tag="ACHJWB_002566"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO4314179.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLINQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16354..17613
                     /gene="rhaA"
                     /locus_tag="ACHJWB_002567"
     CDS             16354..17613
                     /gene="rhaA"
                     /locus_tag="ACHJWB_002567"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4314180.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            17855..18679
                     /gene="rhaD"
                     /locus_tag="ACHJWB_002568"
     CDS             17855..18679
                     /gene="rhaD"
                     /locus_tag="ACHJWB_002568"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4314181.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18689..19003
                     /gene="rhaM"
                     /locus_tag="ACHJWB_002569"
     CDS             18689..19003
                     /gene="rhaM"
                     /locus_tag="ACHJWB_002569"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4314182.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4712 a         4924 c         4815 g         4620 t
ORIGIN      
        1 gcaacatgga tatgctttca ttgcattgaa ttttaaagtg ttttacatga aaaatgactc
       61 ttcatctcga aatccgaacc actcattaat aaccatatac gtgaattttt tcccgtcata
      121 atttacggtt gccccacgcg ccagcgcctc aatctcccat cgctgaggcc tgataccgtt
      181 ctgagcaagg tcaacgcgga tacgggtaat ttgcattcgt tccgaccggg tcagtctggc
      241 cgatggcgct atttcatgcg gttttaacgg gcttccgctt ctttgctgac ggtttggtgt
      301 tctcaggtcg tgttttaatg cgcccctgag cgccctcacg acctccgggt cattccattc
      361 gataacaccg tcatcaacca gattaagcac tgcggcggcg tgctcagaag gtgtgggagc
      421 cggtaacgaa gtatcaccac cggtgagctt tccacagtta ttgacaggac tccgaggcgc
      481 ggcgatgccg ctttttaaag tcaaaggctc cacgaccgga actttcggca caatgcgcca
      541 gtccgtcgtt ctggtgatat gaatatgacg cgcgccgaga tgcggcgcgt aaatgccgac
      601 cactctctcg acttcttcct cgtactcgtt aacgtcatcc gacgggctac gggcgactct
      661 gacagtctgg caatcgcgcg gaacatttgc cccaccctgc gcgctgatat acaacgcaaa
      721 atcgccactg tctgcggcag cacgtgcagc ctcgacgcgt tcgtcaaact catcagcaat
      781 gctgacgccg cgaggcaatt tgcgtagttc acggtaagcc cccattgtcg gcaggccaac
      841 cgttttaaat tgcgggatac gccacgttga cgcccatgcg gtaacagccg ctgcagtgtc
      901 tttcagcggc ctgccggtat cgttatcgag ctgaccatcc agtgcatagc cgtcgatgtt
      961 ttttgaaatg tatttcgcga tatatcccgc tgcaccgccc cggttaaggt gttttgcctg
     1021 aaaacggttt cgcgcagctc ctctttcatc gccatcctct ttgagcgcat agcgacgcat
     1081 gatttcgata atctggttac gctggcgtgg attacaaaaa agcatcatat gccagtgcgg
     1141 tgttccatcg tggtgtggct cgacgactcg caaaccgtag acctgtaaat cattatcctt
     1201 gaatgccgtg cgcatcaggc tccagatacg gcagagataa cgctgcgcat cctttggatt
     1261 aaatgcctca tcgttccagc cgtgatttag ctggacggtt ttactttcgc cttttccgac
     1321 ctgacgtgtc gggtgatact ttgacggcgt ggtcagcgtg ataaacatcc ccacatcacc
     1381 ctctgcggcg gcgtaacgct caataccggc aatggtgttc atcagctcca tccggcgaat
     1441 ttcaggatta gaaatactgc ccatcacctt actgataagg tcgatgcgtt cgccggtttc
     1501 cctgttttcg aggtcacacg atttaagaaa ttccagattt gcctggcggc gcgcacgcac
     1561 atcacgaatg gcgtgtttac tggcataagg agaacggtct ttattgacct ccccgacagc
     1621 aatcagtaac gcctcatgcc agcgcatacg ctggccttta agctgatgag tccaccactc
     1681 atcgttaaac aggcgggcaa tggcagaata tgcctgcctc gtggtcatct gccctttacg
     1741 gtattttttc cagtaaagcg gggaaatatt gaaagcacgt gcagcgccag caacatgacc
     1801 atacaggtga gcctgcgcct catccgtaaa cagcgattct ttttcgccat gcgcatccac
     1861 ccaggcatcg cagatttcct catacatcat gaaaagctgc gatgagatac gggcggcaaa
     1921 ctttttcagc tccttgtcat tcattccagg caggcgcgca tagtggtcac gctctgccag
     1981 aaacagcaac gacgcgtcgg tgttcatttc atggcgctga ttcacgcgct caatgcgcgg
     2041 ccataaacga cgctgaaaag tggatgtgag gaaataaaac ccgtgcaccg ggcttttatt
     2101 gcgccggata tagtcatagc gtgaagtaaa cagcgagcgc aaaaagtaag gcaggcggtt
     2161 aatcgtggat aaaacacctt gcacctgacg catctcgtca cgtgtaaggg gtctttcgcg
     2221 cccgacagcc tcacgtggcg cgttccatgc ataagcaccg gtaaacgtct taccggtgcc
     2281 tgcggcaaat gctgacgaag ggacaaaacg cccggaggct ttaacggcca tgtgtgccaa
     2341 aagcctctga acaacgcttg ctgagttgct caacctgcgc gtttaaatcg gcaaaagatt
     2401 ttgcgcttcc ggtcagaata tcgtgatgca tcaggccgga aacgagctgg cttaatttcg
     2461 ggtaataacc aaccaccgcc agccattcct gaccggcgtt tttaccgctt tccgctctct
     2521 ttttctcgtg gagaataaac tgaaagctgt cactggtaac gacataacgt tcgccaattt
     2581 caatacgaat actcatgccg ttctccggta atgtttgttt tttgcttcaa agactgactg
     2641 gcaggaaaca caacgcgtgg ctgacggata agccgcacga cgggcagcag gtattggcgc
     2701 atcacactct tcgcaaacca gcgcagaagc acagcaatgt tttacccttg ccgcgttaat
     2761 ctggcgctcc agtaattcag cctgttgttc ctgaatgaaa tctacgttgt ccggcattac
     2821 cagttccttt tgtcgttcag tttcttaaat tcatcagcgc aatagctggc gagttctgtc
     2881 gttaatttag tcagttcatc cacggaggag atttgcttgt ggaatacagc gcgtttaaca
     2941 agtaaattga ccacatcaga caggaggttt aattcattct gataaatcgc gataacagat
     3001 tcagttatgt cgcgcttttc tttatcaaga ccaagttgaa taagagacaa atcgccattt
     3061 tccataacgg cgatttttaa ggcgttattc agtaatacaa ctgaatgaga acaggacatc
     3121 aaagcacctc cccgcgagac aatccgatat tgtgaaattt ttccgactcc tgactgagca
     3181 gctcgactat ctccacgcgg gataactccg cctttgtgat atggcgaatc atggcgtcaa
     3241 gatgagaaga aaagcgtgtc gctgcgtcgg cctgtgcttc ggctctggcc tgttgcagca
     3301 gtaatgcgta tttaccgcac ttgttttcag aaactgtatg catgactttc tccaggcaaa
     3361 aagaagcccc gcacgattaa gtgcgttaaa aactctggtt aattatttaa tgcagatatt
     3421 gctctggttt taccgacgtc agaattgtcg gtgcatactc aaacaggctg aataattcac
     3481 gtaatgcacg gaataaagca tcacgccagt aacatgattc ttcattaatt cgccagtatg
     3541 gctggttgaa ttctttttca gtcaatccgg catgcataaa taaagtacga cgctgactga
     3601 ctgttaaaaa actaatatat gcatactcac ttgcgccaac ctgacggcgt tttgagaatg
     3661 ccccacgcaa ttcatcaatt gcacaaacca gccgttcacg ttcgacgtcg ttcatttctt
     3721 caaaacgcat cgttgcgtga cgctgtttta actgcgcatg gaagcaaacc gttagccgtt
     3781 cgcgttccat catctgatta taataatcac atgtatcctg ccagcgaggg acagcaagat
     3841 gcttgccaat tatccggcgc atagctgctg gctgtttttc aacgagattg agcgtcatca
     3901 ctgtcatttc cagaccctcc ggcttttcag aaaggtcaga gcctttttta acggactctg
     3961 ttttttggtg cggataatga ttcccttgcg tcccttcccg tgggtgattg tgaagtcaat
     4021 cgccctgggg ctttcgttac gcaataactg agcaatacaa cgcggctcat tcataatcac
     4081 aaccccatcc acaaaagcca tgcatcacgc tgttcaaccg gtcggttata aaacgcctca
     4141 cgtacagcgc gattaaactc aggaatgaaa acccattttt caccggcacg agccttcggt
     4201 ttgcaaggat cacgcaattc aataattggt aatttatttg ccttcaccat ttcactgaca
     4261 gctgtctttg gcttccctaa taaatcagca aatttatcca catgaaccgc atcaagcgga
     4321 tactgaatca cataattttc agcgtccata tatggtaccc tcataggatc cagccctttc
     4381 taaaccactc aaaaccgttt agacgctggt ttattctcaa atcaatggaa cctatatagg
     4441 ttccagtttt gagggaattt agtccctata taggcaccat gtcaaatgaa attaagcgaa
     4501 aagattaagg ccttgcgtga ggctgaaggg ctaagccaat caaaattctg tgaaatcata
     4561 gagttaccgc taagcacact taaaaaatat gaaggaggaa actttgaacc cggtggcaca
     4621 gctttgctaa aaatcactat gcatcccaca ttccaaaaat atgctctatg gcttatgaca
     4681 gataaaaccg cgccggacgc aggacaaatc gcaccggctc tcgcgcacat tgggccagag
     4741 tcaacagagt ccaaccactc cgcgaaaagg attggctaac tctatataaa gattacattt
     4801 tcaccatttg ctaccaagat ggtgaataca gcgccggagg gctttcttat ggcaattaag
     4861 aagctcgatg atggtcgcta tgaagtggac attagacctc gcggtcgcga cggaaaacgc
     4921 atccgcagga aattcgaaag aaaagctgaa gcactagcat ttgagcgata cacaatcgcc
     4981 aatgccagtc agaaagaatg gggaggccag cgagcagacc gccggacttt gagtgagttg
     5041 ctggacatct ggtggaaata tcacgggcaa aaccacgagc atggaacaaa agagtttaat
     5101 catctactca aaaccatcag cggcataggt gatataccag tgagcaggat gagcaaaagg
     5161 gctttgatgg attatcgttc catgcgacta cgtgatggta tcagtgccgc aacgataaac
     5221 cgtgacatgt accgattatc cggcatgttc acaaaattaa ttcaattgga tgaattttcc
     5281 gggcaacacc caattcacgg actgccgcca ctggcggagg ccaaccctga aatgacgttc
     5341 ctggaaaaag cagaaatcga aaaactgtta aatgttttgg ctggtgatga cttacttgtc
     5401 gcgcttttat gtctgagcac tggaggaaga tggacggaag ttgccacgct aaaaccagca
     5461 cagattacaa attgcagggt taccttcctg aaaaccaaaa acggtaaaaa gcgaaccgtg
     5521 ccgatttctg aggaactgga gaaaaaagtt aaagaggagg ccagcgccaa attattcaaa
     5581 gttgattatg agaaattttg cgggatttta cgcagagtga aacctgatat accacccaat
     5641 caggcaaccc acatcctgcg gcatacattc gcaagccatt tcatgatgaa tgggggcaat
     5701 ataatcgcac tgcaacagat tctgggacat gcgagcattc agcagacgat ggcctatgcg
     5761 caccttgcgc ctgactatct gcaaaatgcc gtcgctctga atccactaaa aggcggagtg
     5821 acgttataaa tttccccctc tgagtgtcca catagtgtcc acactcaaga aattttgaaa
     5881 ccgctccaaa cccttacagc tttcgataag tacctgtttt tacttttgat tgcatgtaag
     5941 taattgataa aaaaaacccc cacatcatgt gggggaagac agggatggtg tctatggcaa
     6001 ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct tcaacgatga
     6061 acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt
     6121 taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga cttttgccat
     6181 ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc gcacagcgtt
     6241 ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc taacattaac
     6301 aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac gctgatgttc
     6361 ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa gttcttcacc
     6421 cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac tgactgccag
     6481 cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa atctttctgt
     6541 cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa
     6601 acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc ggaggtattt
     6661 aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc ctattaaagg
     6721 agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa caggcgcttg
     6781 atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg aagaaaaatg
     6841 gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt atgttgacgg
     6901 cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat gactatctcc
     6961 cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc
     7021 actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa gttgatgcct
     7081 tagtgctgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg gagttaaccg
     7141 gtactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg gtttcccgtg
     7201 aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc tttcgaccgc gctattgata
     7261 tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac ccgtggttta
     7321 aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca gcttaaccgc
     7381 gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt
     7441 acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc gtcaggggct
     7501 gatgattgag cagcatgtcg aagcggagct ggcgaacgat ccgcccaacg atttaatgtg
     7561 gtggcggcgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc gtttgttatt
     7621 ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa
     7681 ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg gccgcgtgga
     7741 actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt atctgattcg
     7801 tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc cgctattact
     7861 gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct ggagtctggc
     7921 aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa acttacgcca
     7981 gcacccggaa ctggaagcgg ggccacagga attccttgcc gcaggtgcca gttttaacca
     8041 gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt ctgatatctc
     8101 tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg
     8161 tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa gcgcaacgtc tggacagcat
     8221 gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa aacgcgctgg ttagcgaaac
     8281 catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg aagccgagca
     8341 aatgggcaag tcgttgacag ttaacttccc gcctgggccg tggccgctgt acggcaatcc
     8401 gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat gctctgcgtt attcccatac
     8461 gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg tggacgacga
     8521 tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct atcggaccga
     8581 tgaagcacgc gatcgtgaat ctggcggtac aggattgggg ctggcgattg ttgaaaccgc
     8641 cattcagcag catcgtggct gggtgaaggc agaagacagc ccgctgggcg gtttacggct
     8701 ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg caggataagg
     8761 cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat
     8821 caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc
     8881 cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg gcggcggaga
     8941 gtaaacggtg atattgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct
     9001 gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca gacactttcc
     9061 ctggcgctat cacgctatac aaccacccca ccttacctgt gttttgcatc aactgcgcta
     9121 tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct
     9181 gaattaatgc ctctccccag cggaaaatat cgcccatata aacattactt tccgtgagac
     9241 cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa
     9301 attcccgcgc ccagtagaga taatgctcac gaggataatg acacagcgct ctgtctggtc
     9361 cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc gtcagcatca
     9421 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
     9481 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
     9541 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag
     9601 gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag
     9661 gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac tttacgtgac
     9721 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
     9781 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
     9841 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
     9901 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
     9961 aacaggatct ccagcaaacc agtctgggca atcacagtca aatcaatggt attgcccagc
    10021 gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc tcgtaattca
    10081 gggtcaaggt tccccagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
    10141 tgcggttcaa acgaggcaat ccctgcagtg cccagaataa tcatcgtcat gagcggcccg
    10201 gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    10261 atggaagcgt aaagtccgcc attggtcata tccatcgccg ccaccagcgc cagcgttgaa
    10321 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
    10381 gcaaccaccc acgcgacggc aatttttgtt accaccagcg taccggattt acgcaatacc
    10441 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    10501 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    10561 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    10621 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    10681 tgcgtcattt ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
    10741 caataaaagt gatcaaaacc acataaattg aaacactgtt ttatttttat cggatcattg
    10801 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    10861 gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt ttttcgccgc
    10921 aaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttaa tgtagtccgg
    10981 acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg
    11041 gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt tagcagtaga
    11101 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    11161 agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
    11221 agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct
    11281 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    11341 gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag
    11401 cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    11461 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    11521 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    11581 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
    11641 gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat
    11701 tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
    11761 actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag atgagtaacg
    11821 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    11881 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg
    11941 tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat ttctgggcgt
    12001 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    12061 ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcatgtcg atgggaattg
    12121 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    12181 atttcgatgt gctgattaat accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    12241 cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc aagatgggca
    12301 ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
    12361 tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
    12421 cactaggcgt cgatccactg tatgtcgctc tgccaagcta tgtcatcatc atgggcggcg
    12481 gcgcgatcat caacctcggt ttctgtttta ttcgtctggc aaaagtgaag gatttgtcgc
    12541 taaaagccga cttctcgctg gcaaaaccgc tgatcactca caatgtgtta ctctcagcac
    12601 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    12661 cggcgcaata tgactacatc agttggatgc tgcatatgag cttctatgta ttgtgcggcg
    12721 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg acgccgtccg gtaacggtgt
    12781 tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc ggcatggcga
    12841 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    12901 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    12961 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgtcgcagat
    13021 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    13081 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
    13141 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    13201 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
    13261 caaacgacac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
    13321 ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgtta aatcccggaa
    13381 tcgccccctg ccagtcaaga ttcagcttca gacgctccgg gcaataaata atattctgca
    13441 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    13501 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg caggccatta ccgcgccaga
    13561 caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc ggataacggt
    13621 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    13681 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
    13741 caaatacgtt gagaagattc gcgttattgc agaaaaccat cccgtccctg acgaatatca
    13801 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    13861 ccacagcgat aggcgatgtc agtaacgctg gcctcgctgt ggcgtagcag atgtcgggct
    13921 ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca atcccgtttg ctgcttaagc
    13981 tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc ccaattcacc
    14041 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    14101 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
    14161 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
    14221 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg atactgccca
    14281 tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    14341 cgatatagca cattggtcag acacagatta tcggtatgtt catacagatg ccgatcatgg
    14401 tcgcgcacaa aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
    14461 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    14521 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    14581 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    14641 acgaggtcag gttcttacct taaattttcg acgggaaacc acgtaaaaaa cgtcgatttt
    14701 tcaagataca gcatgaattt tcaggaaatg cggtgagcat cacaccacca caattcagca
    14761 atttgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    14821 tagcgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    14881 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    14941 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
    15001 taaaaatggg ctgcatagcc agaacggtta tgtcacctgg gatgtggata gcctggaaag
    15061 tgccattcgc cttggattaa acatggtgtg tgaggaaggg attcgtatcg atagcattgg
    15121 gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc gtgtgggcct
    15181 gcccgttgct tatcgcgata gccgcaccaa tggcctaatg gcgcaggcac agcaacaact
    15241 cggcaaacgc gatatttatc aacgtagcgg catccagttt ctgcccttca atacgcttta
    15301 tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg ctcacgctct
    15361 gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg agtacaccaa
    15421 tgccaccacc acacaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
    15481 gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcattgg
    15541 gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
    15601 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
    15661 cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcgctggc
    15721 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    15781 gggcttatgg ctgcttcagc gagtgcttca ggaacggcaa atcaacgatc tcccggcgct
    15841 tatcgccgcg acacaggcac ttccggcctg ccgcttcatc atcaatccca atgacgatcg
    15901 ctttattaat cctgaggcga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    15961 accgatcccg gaaagtgatg ctgaactggc gcgctgcatt ttcgacagtc tggcgctgct
    16021 gtatgccgat gtgttgcatg agctggcgca gctacgcggt gaagatttct cgcaactgca
    16081 tattgtcggc ggcggctgcc agaacacgct gatcaaccag ctatgcgccg atgcctgcgg
    16141 tattcgggtg atcgccgggc ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt
    16201 aatgacgctg gatgaactca acaatgtgga tgattttcgt caagtcgtaa gcaccaccgc
    16261 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagat
    16321 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
    16381 gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa
    16441 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
    16501 aacccggaag gttcgctgac cggtgggatt caagctactg gtaattatcc gggcaaagcg
    16561 cgtaatgcca gtgagctacg taccgatctg gaacaggcta tgcggctgat tccggggccg
    16621 aaacggctta atttacatgc catctatctg gaatcagaca cgcctgtctc gcgcgatcag
    16681 atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    16741 gattttaacc cctcctgttt ttcgcatccg ctaagcgccg atggctttac gctttcccat
    16801 gccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg tcgcgtttcg
    16861 gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat cccggatggt
    16921 atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat
    16981 gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg
    17041 tttggcattg gcgcggagag ctacacggtt ggctccaatg agttttacct ggggtatgcc
    17101 accagccgcc agacggcgct gtgcctggac gccgggcact tccacccgac tgaagtgatt
    17161 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagtcgt
    17221 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc
    17281 agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga cttcttcgat
    17341 gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg
    17401 ctgcgtgcgt tgctggaacc taccgccgag ctgcgcaagt tggaagcggc gggcgattac
    17461 actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa
    17521 atgtattgcc aacgtcacga taccccaacg ggtagcgaat ggctggagag cgtgcgggct
    17581 tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc acgagcgcct
    17641 tatccggcct acgggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg ccctctccct
    17701 gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca ctgctccgga
    17761 cagtcccctc gcccctacgg ggagagggtt agggtgaagg gaacaagcag caccatgtct
    17821 aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc agtcctggtt
    17881 tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg acgagcgcaa
    17941 cggcggtaac ctgacgctgc gcctggatga cgccgatatc gcgccttata aagacaactt
    18001 ccatgcacaa ccgcgttata tcccgctcag ccaacccatg cctttactgg caaatacacc
    18061 gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagcttgatc ctgcagctaa
    18121 cttaggcgtc gtaaaagtcg acagcgacgg cgcgggctac catattctct gggggttaac
    18181 caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact gcgagcgcat
    18241 taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca acctgatcgc
    18301 cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt gggaaggcag
    18361 caccgagtgt ctggtggtat tcccggatgg cgttggcatt ctgccgtgga tggtgcccgg
    18421 cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc tggtgttgtg
    18481 gcccttccac ggcgtcttcg gcagcgggtc gacgttggat gaaacattcg gtttaatcga
    18541 caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg gcatgaaaca
    18601 gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta caccactagc
    18661 cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca tgcaggttaa
    18721 ccccgacgcc cacgaagagt atcagcgtcg gcataacccc atctggccgg aactggaagc
    18781 agtgttgaaa tctcacggtg cgcataacta cgccatttac ctcgacaaag cgcgtaatct
    18841 gctgtttgcc acggtagaga ttgaatctga agaacggtgg aatgcggtag ccagtactga
    18901 tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc cggataacag
    18961 cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca atgtgccgga
    19021 gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat t
//
LOCUS       DAZFAH010000078        18874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFAH010000078 DAZFAH010000000
VERSION     DAZFAH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.078"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ACHJWB_002570"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ACHJWB_002570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4314183.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="ACHJWB_002571"
     CDS             834..1262
                     /locus_tag="ACHJWB_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4314184.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="ACHJWB_002572"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="ACHJWB_002572"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4314185.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEIISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="ACHJWB_002573"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="ACHJWB_002573"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4314186.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ACHJWB_002574"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ACHJWB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4314187.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGREPRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ACHJWB_002575"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ACHJWB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4314188.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ACHJWB_002576"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ACHJWB_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4314189.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ACHJWB_002577"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ACHJWB_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4314190.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ACHJWB_002578"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ACHJWB_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4314191.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGT
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="ACHJWB_002579"
     CDS             complement(8281..9465)
                     /locus_tag="ACHJWB_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4314192.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9757..10749)
                     /gene="proX"
                     /locus_tag="ACHJWB_002580"
     CDS             complement(9757..10749)
                     /gene="proX"
                     /locus_tag="ACHJWB_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4314193.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDSWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10807..11871)
                     /gene="proW"
                     /locus_tag="ACHJWB_002581"
     CDS             complement(10807..11871)
                     /gene="proW"
                     /locus_tag="ACHJWB_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4314194.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11864..13066)
                     /gene="proV"
                     /locus_tag="ACHJWB_002582"
     CDS             complement(11864..13066)
                     /gene="proV"
                     /locus_tag="ACHJWB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4314195.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="ACHJWB_002583"
     CDS             complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="ACHJWB_002583"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4314196.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="ACHJWB_002584"
     CDS             complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="ACHJWB_002584"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4314197.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIANTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKMAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="ACHJWB_002585"
     CDS             complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="ACHJWB_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4314198.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="ACHJWB_002586"
     CDS             complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="ACHJWB_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4314199.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17407..17736)
                     /locus_tag="ACHJWB_002587"
     CDS             complement(17407..17736)
                     /locus_tag="ACHJWB_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4314200.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTATAVGIFIGA
                     LLSMRKS"
     gene            17888..18232
                     /locus_tag="ACHJWB_002588"
     CDS             17888..18232
                     /locus_tag="ACHJWB_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4314201.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="ACHJWB_002589"
     CDS             complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="ACHJWB_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4314202.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4746 a         4778 c         4880 g         4470 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctactggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag ctaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gttgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagataa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgaagcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacgcctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa attttaaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga atgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgcctccgcc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttc ggtgcggggt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagcgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc gcggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgctttcgcg
     5941 agggcgattt tttgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagaacatcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatttgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcata
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgcca cgccaaccag taacccaagc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcctggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gcagcttcaa cggcgggata accttgcagc aagccgctgc cggagaatgt
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgcg gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaatgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taaccagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcgcagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcca gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgagat tcgccagcaa
     9001 tccggcaacg gtccgtgcga gtaagatccc caacagcaga ccgctcataa tagtgccaac
     9061 cactttgccg cgtttgtccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgacagag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaattg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgagcgt aatagttact ggcgaccgcc agaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatatggg atgaaagata
     9541 gattgttgaa atgtgcgatg tggtttgtag gcatgataag acgcgccagc gtcgcatcag
     9601 gcattgtgca cagaacggcg gatgcggcga aaacgcctta tccgccatac ggaatgagtg
     9661 tattttgtag gcatgataag acgcgtcagc gtcgcatcag gcattgtgta tagagcggcg
     9721 gatgaggcaa aaacgcctta tccgcctgaa taaaaattac ttctgcgctg ccagcgcctc
     9781 attcacccag ccatcgaact gctgctggtg ggctttgatc cagctatcaa cgtggccctg
     9841 aatatcgcct tctgaggctt tgccgtcatg cataatggcg ttctgggcgt taatatcggc
     9901 cactggcaac tgcataatgg caaacagttt ggctgccgcc ggatttttct cggtccaggc
     9961 tttgttggca acgatatgca tggtgctgac cgggaagccg taattcgcgc cattcggcag
    10021 tttggtatcg gcatttttat cgcccggcag tgcggagaac ggcacctgca accagaccac
    10081 atctttccct ggcttcagct cattactcac ccagtacggc gtccaggtgt aataaaacac
    10141 cggtttgccc tctttgtagc gactgatggt gtcggccatc atcgctgcgt agttcccctg
    10201 attatgcgtc acggtgttgg tcagttcata cgcggcaagc tggtggttga tcgcaccttc
    10261 gcagccccag ccagggttac aaccggttaa atccgcttta ccgtcgccgt tggtatcgaa
    10321 cagtttggcg atcttcggat ctttcagttg tgcgatgttg gtgattttgt actggtcggc
    10381 ggttttctta tcgatcaggt aaccctgtgc cgcgccgtta acaaataccc cttcacgata
    10441 aaatttctta tcgccaccgg cagcttcgta catgttgtca tgcagtggcg tccagttcac
    10501 ggcggtgaag gttgcatcgc cggaagcaag cgaggtgtag ccaacgttgt aatctacttc
    10561 gctgggtttg ttgacggtat agcctaattt ctccagcgca cggctgacca gcagcgtctg
    10621 gaaggtttct tcggtgatgg tgctttgaac tggatttaca gtaatacctt tgcccggcag
    10681 atcggcagca aaagtttgtg tagagataag cgtggcaaac gctgtcgcaa aaagtacgct
    10741 atgtcgcatt gttattcctt tttttggcag ggtgataatg cccggcgtca ccgggcaagt
    10801 gcagagttac ttaatgaatg ggcgggtcag cagaccaaca gggccagtgg tgtaccagcg
    10861 acggttgccg cgactgcgtg agtcgcgccc aacggcctgc gtcaggcgat cgaggataat
    10921 ggcgaggatc acaatcccga cgccgccaac ggtggcaagc cccatatcca gacgaccgat
    10981 accgcgaagt accatctgac ccagcccgcc gacggcaatc atcgaggcaa tgaccaccat
    11041 agaaagggcc agcatcagcg tctggttaac gcccgccata atggtcggca tcgccagcgg
    11101 taactgaact ttgaacagca tctggcgcgg gctggcaccg aatgagcgcg aggcttcaat
    11161 cagatccgcc ggaacctggt taatccccag aatggtcaga cggataatcg gcggcagagc
    11221 aaagatgatc gtcaccacca cgcccggcac gttaccgata ccaaacagca tgacgattgg
    11281 caccagataa acaaacgctg gcgtggtctg catggcatca agcagtggac gaataatttt
    11341 cgccgctcgt ggacttctcg ccagccagat ccccaacggc aaaccgatga cgatacagaa
    11401 cagcagggcg gttaacacca gcgccagagt caccattgcc tgcgaccagg caccgattgc
    11461 gccgatggca atcagcgaaa ccagcgtcgc cacacccatt ccgaccccgg aaatctgcca
    11521 ggcgatgaga gcgaaaacga taatcgccac cggtgcgggc atacccagca gcaattgctg
    11581 gaaaccgttg aggatataat caaccggaac gcgcacgccc tggaagacgg gacggaaatg
    11641 ggtaacaacc cagtcgatcc cttcagtgac ccaactgtcg agcgggatca gcgttttatg
    11701 gaacggatcg agaatattaa aatgctcgac gtttggcgca ggcgtactgg tcagccagtc
    11761 agcaccacca ccgtcagtcg gtgcagtcgc cggtgtaccc caggcgtctg cggattgcac
    11821 ggcactgtcc gccgctggcg tggtatccca cggattattt tgatcagcca ttatttaccc
    11881 cctcacgatc taaagcgcgc agcagcattc ctttcgaaat gatgccgaca tactgttggt
    11941 cctcgtcgac cacgggcacc gcacagggtg cctgtccgac atgagagagc aactcgctaa
    12001 gaggcgtttg tgcatcgact gctaacggcg catcaatcag cgccgcatca agaccttgct
    12061 gctgcgttaa cgcggtttta agcgaatcga tggaaactgc gccgacaaac ttattaccgc
    12121 gttcgataac gtagccatat tcgcgatctt catcctgcaa taatttcagt gccgaacgtg
    12181 ggccgaagcc aggggtttta cgaattaagc cattcggtgt ccggcgggca atatctttcg
    12241 cactgaatac ctgactaata tcaacgccac ggaagaaggt acggacataa tcattcgccg
    12301 gattattgag aatttcatcc ggtgtgccga cctgtaccac ttcaccattt tgcataatgg
    12361 caattcggtc gccaatacgc atagcctcat cgagatcgtg ggaaataaag acaatggtgc
    12421 gctgatgttt cgcctgtaat tttaccagct catcctgcat ctcggtgcga attaatggat
    12481 cgagagccga gaaggcttcg tccattaata atatatccgg attaatcgct aacgcgcggg
    12541 ctaatcccac acgttgacgc atcccgccag agagttcatc cgggtagctg tgggcataat
    12601 tttccagccc gacctgacgc agtgcatcaa gggctttttc ccggcgttct tcggcattaa
    12661 ttccggccaa ttccataccg aacgcagtat tgtccagcac ggtcatatgc ggcattaagg
    12721 caaaggactg gaagaccatc gcaatctttt ttctgcgcac ctcacggagt tcggcatcgg
    12781 atattttggc aatatccaca ccatcaatca gcacttgccc gcgggtgggt tcaatcaggc
    12841 gattgagaag gcgtaccatt gtggatttac ccgagccgga taatcccatg atgacaaata
    12901 tctcgccttc ttcaatggcc agactggcgt cttttacgcc aagcgatagc ccagtttttt
    12961 ccagaatttg ttctttcgaa agtccttgtt cgatatattt gaacgctcgc tgtggatgct
    13021 cgccaaatat tttataaaga tttttaattt ctaatttaat tgccatgcaa tagaaagatt
    13081 cctttatttg tttatgtcga tatattaccc aatggaaata gtcacttttt tctaccctaa
    13141 catactgaga atctgaggca acccctgagg gcaaatgtgg catgaatatt gatgcgagca
    13201 aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt
    13261 ttgtcataga tagcgaaaaa acgcctgaat attgtagttt caggcgaata atgcaacaga
    13321 gtatcagtgt agatcactac agaatatttg cgtgataaaa agctatttgg cgggaattat
    13381 ttccctgctg cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat
    13441 cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa aagaaatcgt
    13501 gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt
    13561 ctgcgggaaa taacggttcg tagcccagat tcatcaaagc cttattggcg ttgtaacaga
    13621 gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca
    13681 actcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt
    13741 gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga
    13801 ctgcttcatc gcggataatc agacggatca gatctgcggt attggtcagc tttccgcggc
    13861 tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac
    13921 tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt
    13981 ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct
    14041 gacacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga
    14101 tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc gaaggcgcgc
    14161 cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt
    14221 gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc ggcacctttt
    14281 ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata
    14341 tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac
    14401 aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cgaatgtaat
    14461 agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg
    14521 tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg atcgacatgg cgagtcgctt
    14581 ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcatcctga tacagcgcca
    14641 gattctcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc
    14701 gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga
    14761 tagaaccggt tggcggcacc gcctgaagat tctggttata aatgccgtag cgcatcacgt
    14821 cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt aataccgcta cgggcaaaca
    14881 gttcgccaac tttcgccatt ttcggctgcc agttcccttg cagatattgg ctgaaatatt
    14941 caccactggc atagcgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgtgcca
    15001 gcaacatcga ggtacgcagt gcgtgccagg tgatggtata gaaatagaga ttggtgaaat
    15061 ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagataaccg tgtaaattca
    15121 tctgccccag cccgatggcg tgtgaggcgg catttccggc ttcgatggac ggcacgctgc
    15181 ggatatgact catatctgac accgccgtta aaccgcgtac ggcagtctct accgtgcggg
    15241 caaagttggg ggaatccatg gtgttggcaa tattcaacga acctaaattg caggaaatat
    15301 catggcctgt acgggcatag tcgagattct cgtcatactc tgaggcgctg ttaacctgca
    15361 aaatttctga gcagagatta ctcatattta tgcgcccggc gatagggtta gcacggttta
    15421 ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct gccagtcgct
    15481 ggaagaaatc acgggcgttg aggtattttt tgcgaatgcg ttcatcggca accagttcgt
    15541 catagtgttg gctgatggcc acatcggcaa acggcttgcc ataaactcgc tcaacgtcat
    15601 agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga
    15661 tcaccacgcc cagcgacagt gttttaatgc ggattttctc gtcggcattt tcccgtttcg
    15721 tatcgagaaa acgcagaata tcgggatgat gggcatgtaa atagactgca ccagccccct
    15781 gacgagcgcc gagctggttg gcataggaaa atgcgtcttc cagcattttc atcaccggaa
    15841 tcacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttcc cgcagattcg
    15901 acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa tttaccgccc
    15961 ggccaatcga ctccatattg tcttcaatac gcagcaaaaa acaggaaacc agttcgccgc
    16021 gctgctgctt accacagttg aggaatgtcg gtgttgcagg ctgaaagcgt cctgacagca
    16081 tctcatcggt cagttgcaac gccagtgttt catcgccttg tgccagcgtt agcgccacca
    16141 tcgtgactcg atcggcaaaa tcttccagat aacgcttacc gtcgaatgtc ttcaacgtat
    16201 agctggtgta aaacttccat gccccgagga atgtatggaa acgaaaaccg ctggtgtgtg
    16261 cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat
    16321 agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt
    16381 tcggacgcac atgcgtcgca ataaaggcgt caacggcctg gcggtcttta tcgaactgaa
    16441 tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa tccatcgttt
    16501 cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt
    16561 ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt
    16621 ctgagcaatc acatctccgg cacggccata cgcctcacca aagttgcgat tacccgaagc
    16681 aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctgtcg
    16741 tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc
    16801 gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca gtcctaaacg
    16861 ttcgataaaa cgctgcgtat tttcggagct actggaaaag tagacgagct ggctcatgca
    16921 ctggccgcgt gtggcgctgg atgcagacga ttgatcatgt ccgggcggaa accagaccag
    16981 ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg cagcgcttcc
    17041 gccgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc
    17101 gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt aatgcgcatg
    17161 attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct
    17221 gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa
    17281 tagtggtcga aattaccctg gatatgagcg tgacggggta gggggatttt tgtgattcac
    17341 caggcaaaaa gaaaccccga agacaggctt cggggtcaaa gacgcgtatt tattatcatt
    17401 tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagttgc
    17461 tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc
    17521 gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa
    17581 tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt
    17641 taatacagat tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc
    17701 atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg
    17761 taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt
    17821 tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg
    17881 atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac
    17941 tgtcgatgtg gtaacccaga aagcgtatgg ttaccgccgt ggggaaaatt atgtctatgt
    18001 taatcgcgaa gcgcgaatgg gacgtaccgc tctggtaatt catccaacat taaaagaacg
    18061 cagttcaaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt accagcaatt
    18121 tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg gcttcagttc
    18181 gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga
    18241 ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa cggctgactt
    18301 taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa
    18361 caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca
    18421 agatcgccac atacaccggt gactggaacg tcacataagc caggatatca gccagatttt
    18481 ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaacagatcg cggtacatac
    18541 cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact
    18601 gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa
    18661 caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc
    18721 taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat
    18781 tgccatctgc attttcgcat agcgtatatc gctggctatg accattcaat tagtgaaaaa
    18841 tttttattta gtcagttttt gtagataaaa tatt
//
LOCUS       DAZFAH010000079        18586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFAH010000079 DAZFAH010000000
VERSION     DAZFAH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.079"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..359)
                     /gene="yneK"
                     /locus_tag="ACHJWB_002590"
     CDS             complement(<1..359)
                     /gene="yneK"
                     /locus_tag="ACHJWB_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO4314203.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFR"
     gene            complement(437..1318)
                     /gene="yneJ"
                     /locus_tag="ACHJWB_002591"
     CDS             complement(437..1318)
                     /gene="yneJ"
                     /locus_tag="ACHJWB_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4314204.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1419..2807
                     /gene="sad"
                     /locus_tag="ACHJWB_002592"
     CDS             1419..2807
                     /gene="sad"
                     /locus_tag="ACHJWB_002592"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4314205.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDHRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2871..3797
                     /gene="glsB"
                     /locus_tag="ACHJWB_002593"
     CDS             2871..3797
                     /gene="glsB"
                     /locus_tag="ACHJWB_002593"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4314206.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3797..4156
                     /gene="yneG"
                     /locus_tag="ACHJWB_002594"
     CDS             3797..4156
                     /gene="yneG"
                     /locus_tag="ACHJWB_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4314207.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4295..5713
                     /locus_tag="ACHJWB_002595"
     CDS             4295..5713
                     /locus_tag="ACHJWB_002595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4314208.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRMLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5940..7391
                     /gene="uxaB"
                     /locus_tag="ACHJWB_002596"
     CDS             5940..7391
                     /gene="uxaB"
                     /locus_tag="ACHJWB_002596"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4314209.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            7598..8512
                     /gene="yneE"
                     /locus_tag="ACHJWB_002597"
     CDS             7598..8512
                     /gene="yneE"
                     /locus_tag="ACHJWB_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO4314210.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(8516..9274)
                     /gene="tam"
                     /locus_tag="ACHJWB_002598"
     CDS             complement(8516..9274)
                     /gene="tam"
                     /locus_tag="ACHJWB_002598"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO4314211.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALRDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9331..9621)
                     /gene="lsrG"
                     /locus_tag="ACHJWB_002599"
     CDS             complement(9331..9621)
                     /gene="lsrG"
                     /locus_tag="ACHJWB_002599"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO4314212.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            complement(9645..10520)
                     /gene="lsrF"
                     /locus_tag="ACHJWB_002600"
     CDS             complement(9645..10520)
                     /gene="lsrF"
                     /locus_tag="ACHJWB_002600"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO4314213.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            complement(10547..11482)
                     /gene="lsrB"
                     /locus_tag="ACHJWB_002601"
                     /pseudo
     CDS             complement(10547..11482)
                     /gene="lsrB"
                     /locus_tag="ACHJWB_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            11469..16122
                     /locus_tag="ACHJWB_002602"
                     /pseudo
     CDS             11469..16122
                     /locus_tag="ACHJWB_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            16331..16597
                     /gene="hipB"
                     /locus_tag="ACHJWB_002603"
     CDS             16331..16597
                     /gene="hipB"
                     /locus_tag="ACHJWB_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO4314214.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            16597..17919
                     /gene="hipA"
                     /locus_tag="ACHJWB_002604"
     CDS             16597..17919
                     /gene="hipA"
                     /locus_tag="ACHJWB_002604"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO4314215.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            18231..18439
                     /locus_tag="ACHJWB_002605"
                     /pseudo
     CDS             18231..18439
                     /locus_tag="ACHJWB_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4668 a         4348 c         4860 g         4710 t
ORIGIN      
        1 tggcgaaaat tatctaaata aatagggctt aaatttatta ctacctgaaa ttcaatattt
       61 tctttgtgtc ttccattctt gacgggataa gtgagtttaa actgaacctt ttcattgtat
      121 agcgtgtcgt taccccgaaa agaagtcctc tgaatatatg ataagttatc aaggttatta
      181 aatatctttt ttgtggctga gttgatgttt aaagacgtgc gccactcttc atcacatatt
      241 attgtactta gcgcatcaat aaagtccttt aaaacttctt ttacctgagg tgcaatatta
      301 cgtacgggaa aatcatcagg aagagatatg taattactga tgcttactgg cgtaaccata
      361 tttgccccat atagtcataa gtcatcggaa catcactata tagggcattt gagctgcaag
      421 aactatcaaa aatagctcat tggtaaccct gttttgccga gtcaggcaca tccagtagtt
      481 gaataaatgc ctcaagcgga cgcgttttcg caccgcgacg ccagaccagc caggttgtta
      541 accaacgcca ttgctcagct aacggccacg cttcaacctg gtgatgcccc ggcatacttt
      601 ccagcataga gcgtggaata agcgcaatgc ctgctcctgc gaccacacag gccaacatcc
      661 cgtgatacga ctccatctcg tgaatagttc ccggagcggc accgtcagca tgaaaccagc
      721 tctcgaagtg gcgacgatac gaacaattgg cgcggaaggc ataaatgtta ctgccgttaa
      781 cctgactggc gcggattact ggcgcatgac cttgtggcgt gacgatcatc agctcctcgc
      841 ggtataccgg tatcccgtca atggcagtat ggttaatggg cccatcaata aatgccgcat
      901 tcagttttcc ctccagcact ccttccagca tagtgccgga agggccggtg gaaagtgaaa
      961 actgaatttt gggataacga tggttgaatt ccgccagcgt ggccggaatg cgcactgctg
     1021 cggtgctttc cagcgaacca agagaaaata aaccttgcgg ctcatcgcca gcgacaacgc
     1081 tccgcgcttc atccactaac gcgagaattt gttggctata gcgtaaaaag ttatgcccgg
     1141 caggagagag acgtaaacgc tgattctcgc gaataaacag atctaccccc agttctgttt
     1201 ccagctggcg tagacgggta gtgagattcg acggtacgcg atgcactttt gctgcagcct
     1261 gggtaatgct gccagcttcg gcaaccgcgt tgaacatctc cagttgggtc agatccataa
     1321 cattctcgat tcgtgaataa gtggcttaat attattcatt ttaaagcaag agtaaatctg
     1381 cgtatcttca taccatgact cataaaggag ataccccgat gaccattact ccggcaactc
     1441 atgcaatttc aataaatcct gccacgggtg aacaactttc tgtgctgccg tgggctggcg
     1501 ctaacgatat cgaaaacgca cttcagctgg cggcagcagg ctttcgcgac tggcgcgaga
     1561 caaatataga tcatcgtgct gaaaaactgc gtggtatcgg taaggctctg cgcgcccgta
     1621 gcgaagaaat ggcgcaaatg atcacccgtg aaatgggcaa accaatcaat caggcgcgcg
     1681 ctgaagtggc gaaatcggcg aatttgtgtg actggtatgc agaacatggt ccggcaatgc
     1741 tgaaggcgga acctacgctg gtggaaaatc agcaggcagt tattgagtat cgaccgttgg
     1801 ggacgattct ggcgattatg ccgtggaact ttccgttatg gcaggtgatg cgtggcgcgg
     1861 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
     1921 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
     1981 ggctgaatgc cgacaacgac ggtgtcagtc aaatgattaa agattcgcgc attgctgctg
     2041 tcacggtgac cggaagtgtt cgtgcgggag cggctattgg cgcacaggct ggagcggcac
     2101 tgaaaaaatg cgtactggaa ctgggcggtt cggatccatt tattgtgctt aacgatgccg
     2161 atctggaact ggcggttaaa gcggcggtag ccggacgtta tcagaatacc ggacaggttt
     2221 gtgcagcggc aaaacgcttt attatcgaag agggaattgc ttctgcattt accgaacgtt
     2281 ttgtggcagc tgcggcagcc ttgaaaatgg gcgatccccg tgatgaagag aacgctctcg
     2341 gaccaatggc tcgttttgat ttacgtgatg agctgcatca tcaggtggag aaaaccctgg
     2401 cgcagggtgc gcgtttgtta ctgggcgggg aaaagatggc tggggcaggt aactactatc
     2461 cgccaacggt tctggcgaat gttaccccag aaatgaccgc gtttcgggaa gaaatgtttg
     2521 gccctgttgc ggcaatcacc gttgcgaaag atgcagaaca tgcgctggaa ctggctaatg
     2581 atagtgagtt cggcctttca gcgaccattt ttaccaccga cgaaacacag gccagacaga
     2641 tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg ttattgtgcc agcgacgcgc
     2701 gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg tgagctttcc catttcggct
     2761 tacacgaatt ctgtaatatc cagacggtgt ggaaagaccg gatctgacgc gacatattca
     2821 gctctgatat actcgcaggt cttttcagac ctgcggtcca ggagtagaaa gtggcagtcg
     2881 ccatggataa tgcaatttta gaaaacatct tgcggcaagt gcggccgctc attggtcagg
     2941 gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga cggttcccga ttgggcattg
     3001 ctatctgtac cgttgacgga cagctttttc aggccggaga cgcgcaagaa cgtttttcca
     3061 ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat gcgtcattac tccgaagagg
     3121 aaatctggca acgcgtcggc aaagatccgt ctggatcacc gttcaattct ttagtgcaac
     3181 tggaaatgga gcagggtatt ccgcgtaatc cgtttattaa tgctggtgcg ctggtggttt
     3241 gcgatatgtt gcaagggcga ttaagcgcac cacggcaacg tatgctggaa gtcgtgcgcg
     3301 gcttaagcgg tgtgtctgat atttcatacg atacggtggt agcgcgttcc gaatttgaac
     3361 attccgcgcg aaatgcggct atcgcctggc tgatgaagtc gtttggcaat ttccatcatg
     3421 atgtgacaac cgttctgcaa aactactttc attactgcgc tctgaaaatg agctgtgtag
     3481 agctggcccg gacgtttgtc tttctggcta atcaggggaa agctattcat attgatgaac
     3541 ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct gatggcgacc agtggtatgt
     3601 accagaacgc gggggagttt gcctggcggg tggggctacc ggcgaaatct ggcgttggtg
     3661 gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc tgtctggagt ccggaactgg
     3721 atgatgcagg taactcgctt gcgggtattg ccgtccttga acaattgacg aaacagttag
     3781 ggcgttcggt ttattaatgc agtctctcga tccactcttc gcgcgtttat cccgttcaaa
     3841 atttcgctct cgctttcgtc tgggcatgaa agagcgtcag tattgcctgg agaaaggcgc
     3901 gccagtcatc gaacaacatg cggcggattt tgtcgctaaa cgtcttgctc cggcattacc
     3961 ggctaatgac ggtaagcaaa cccccatgcg cgggcatccg gtgtttatcg ctcagcacgc
     4021 caccgcgacc tgctgtcgcg gttgtctggc taagtggcat aacattccgc agggtgtttc
     4081 gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc tatcactggc tggttgtaca
     4141 gatgaatcag ccatgagaat tgcggaaaac gataaaatag cattcccagg gtacctttat
     4201 ttgccgccgc tgttagcgaa catgctatcc tttatgagaa tttacttatt acgttgagat
     4261 aattgccacc cgcaattgta tggacagata attaatgcat gtacacccca tttcaacttt
     4321 ccgtttgttc caggaaggtc atctgctacg taatagcatc gctatttttg cgctaaccac
     4381 gctgttctat tttattggtg cagagttacg gctggttcac gaactttctc ttttctggcc
     4441 gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc tggcttaatc gactgcatta
     4501 ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc ataaccaccg aatggggact
     4561 ggtttcactg gttatcaatt tctccaatat gatgtttatt gttaccgtcg ccttactggt
     4621 cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca gtaagtgcct tacggctatt
     4681 taattactgt ctcattgccg cattattatg cgctattgtc ggggcgattg gttcggtcag
     4741 tattgatagt ctggatttct ggcctttgct tgcggactgg ttcagtgagc aattctcaac
     4801 gggcgtgttg atcgtgcctt gtatgctgac gttggctatt cctggagtgc tgccgcgctt
     4861 taaagcagaa cagatgatgc ccgccatcgc gcttattgtg tcggttattg cctcggtagt
     4921 cattggcgga gcggggagtc tggcgttccc gctgcctgca ttaatctggt gtgcagtgcg
     4981 ctatacgccg caggtaacat gtctgttgac ctttgtcacc ggtgcggtgg aaatcgtact
     5041 ggtggcaaat tcggtgattg atatctcggt cggttcgccg ttctccattc cggaaatgtt
     5101 ctccgcacgt ctcggtattg ccacgatggc gatatgccca attatggttt cttttagcgt
     5161 ggcagcgatc aattcgctaa tgaagcaagt tgcgctgcga gccgactttg attttctgac
     5221 tcaggtttac tcacggtccg gtctttatga ggcgctgaaa agtccatcgc tgaaacagac
     5281 acaacatctg acggtcatgc tgcttgatat cgactatttc aaaagcatta acgataacta
     5341 tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc cggcatattc agaagattgt
     5401 cggtgataag gggctggtgg cgcgcatggg cggcgaagaa tttgctgttg cagtgccgtc
     5461 ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc cgtaaaggcg ttgaactgca
     5521 accattcacc tggcaacaaa aaacgcttta tctcacggta agtattggcg tcggtagtgg
     5581 tcgcgcatcg taccgaatgc tgaccgatga ttttaataaa ttgatggtcg aagccgatac
     5641 gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc agcactatgc gttacggtga
     5701 ggaagttgtc tgacatcttt attcattccg cgcgatttga atgcaatgtg ttctaccact
     5761 ttatgatcca ggaaaaggcg tatttcctcg attaaaccat gatccgcgcc acacttttct
     5821 cataaaactg ctcattaatc atcaggagtt gatcaaaaca ggggttcggc gtattaaagt
     5881 ttgctaacct gtcgtaccaa ttcaaacagg ttgtatgact aatcagaagg gaacccattg
     5941 tgaaaacact aaatcgtcgc gattttcccg gtgcacagta tccagaacgt atcattcagt
     6001 ttggtgaagg taacttcctg cgcgcctttg ttgactggca aatcgatctc ctgaatgagc
     6061 acaccgatct gaattctggt gtggtcgttg ttcgtccgat tgaaacttca ttcccaccgt
     6121 cactgagcac gcaggatggt ctgtatacca ccattatccg tggcctgaat gagaaggggg
     6181 aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg tgaaatcagc gtctacagtg
     6241 aatacgatga attcctgaaa ctggctcaca acccggaaat gcgctttgtt ttctctaata
     6301 ccaccgaagc gggtatcagc taccatgcgg gcgataaatt cgatgacgcg ccagcggtaa
     6361 gttatccggc aaaactgacc cgtctgctgt tcgaacgttt tagccatttc aacggtgcgc
     6421 tggataaagg ttggatcatc atcccgtgtg agttgatcga ctacaatggc gacgcgttgc
     6481 gtgaactggt gctgcgctat gcgcaagagt gggcactgcc agaagcattt attcaatggc
     6541 tggatcaggc taacagcttc tgttctacgc tggtggaccg tatcgttacc ggttatccgc
     6601 gcgatgaagt ggctaaactg gaagaagagc tgggttatca cgatggtttc ctcgacaccg
     6661 ctgaacactt ttacctgttt gtgattcagg ggccgaaatc cttagcgact gagctgcgcc
     6721 tggacaaata tccactcaac gtgctgattg ttgacgatat taaaccgtat aaagagcgca
     6781 aagtggcaat cctcaacggt gctcacaccg cgctggtgcc ggtggcgttc caggcagggc
     6841 tggataccgt aggtgaagcg atgaacgatg ctgaaatttg cgcattcgtt gaaaaagcta
     6901 tctacgaaga aattattccg gtactggatt tgcctcgtga tgaattggaa tctttcgcca
     6961 gtgcagtcac cgggcgtttc cgtaacccgt acattaagca tcagctgctg tctatcgcgc
     7021 tcaacggtat gaccaagttc cgcacccgca tcctgccgca gctgctggca gggcagaagg
     7081 caaaaggcac acttccggcg cgccttactt tcgcattagc ggcattgatt gccttctatc
     7141 gcggtgagcg taacggagaa acatatccgg tacaggacga tgcacactgg ctggaacgtt
     7201 atcagcaact ctggagccag catcgtgacc gtgttatcgg cacccaggaa ctggtagcga
     7261 ttgttttggc agaaaaagac cactgggagc aggacctgac gcaagtgcct ggtctggtcg
     7321 agcaggttgc taatgacctg gatgcgattc ttgaaaaagg gatgcgcgaa gctgtacgtc
     7381 cgttgtgcta ataacaccgt tacttctgaa cccggttttg accgggtttc ttatttcact
     7441 atattagtta caacatccta tgtgccttta tcattaacag gtgccacatg cagcgcctgt
     7501 taatgttgta agcttgtagg taatttgtaa gtagcgtctt gagaatgtga tcgtaatcac
     7561 gtttaatagg gcgacttttc cacgcctgaa gataaccatg attgttcgtc cacaacaaca
     7621 ctggctgcgc cgtatttttg tctggcacgg ctcagtatta tccaagatat cctcgcgctt
     7681 actactcaat tttctctttt ctatcgctgt tatattcatg ctgccctggt acacgcatct
     7741 gggcatcaaa ttcaccctcg caccgttcag cattctcggt gtcgccatcg ccatttttct
     7801 tggttttcgt aataatgccg ggtacgcccg ttacgttgaa gcgcgaaaac tttgggggca
     7861 gttgatgatt gcctcacggt cgttactgcg cgaggtaaaa acgacattgc cggattcggc
     7921 aagtgtaagg gagtttgccc ggctgcaaat cgccttcgcc cactgtttac gcatgacatt
     7981 acgcaaacag ccacaggcgg aagtgctggc tcattatctc aagactgaag atcttcagcg
     8041 tgtactggct tcgaactctc cagctaaccg tatcttgtta ataatgggag aatggttggc
     8101 ggttcagcgc cgcaatggac agctttcaga tatcctgttt attagcctca acgatcggct
     8161 taatgatata tcagcggtcc tggcaggatg cgagcgcatt gcctatacgc caattccctt
     8221 tgcctacacc ctgattttgc atcgtaccgt ttatctgttt tgtatcatgc tgccgttcgc
     8281 gctggtcgtg gacctgcatt acatgacgcc ttttatctct gtgctgattt cctacacttt
     8341 tatttcgctg gattgtctgg cggaagaact ggaagatccg tttggtactg aaaacaatga
     8401 tttaccactg gatgccatct gcaacgctat tgaaattgac ctgttacaga tgaacgatga
     8461 agccgaaatt ccagcaaaaa ttcttcccga tcgtcattac cagctgacgt gatatttact
     8521 ccatacgccg ggcaacaata aaaagacgcg gaaatgccag cagtatctgt ccgttctctt
     8581 gcagtggata ctgctcttcc agcatctgat ggtagcgctt aagaaaaagc tgctgttcgc
     8641 tctcggtcag atcctgtaac cacggacgta atccagtggc agtcacccaa tcgataatcg
     8701 cctggtgcga cggcatctgg tgatagtagg tggttcgcca gatatcgacc tcacatccgg
     8761 cttcgctcaa aatatcgtag taagcatgaa cgccagccaa cggctcacgc ccgcgatccg
     8821 ggtagttttg ttcccaggca acttcgcgca tgagcacatg ggtcggctcc agccagttat
     8881 ctggcatctg tactgccagc acgccctgcg gattaagtaa agaaaccaag tgaggaaaca
     8941 attcgtagtg gtcgggcagc cattgcagtg aggcattagc aaaaatcaga tcgagtgcct
     9001 gttccggttg ccagttgcgg atgtctgctt ccacgaactg gcagtctcgc aaagcactgc
     9061 gcgcttcagc aatcatcgcc ggagacgagt cgatgcctgt tatcctggcc gcaggccaac
     9121 gttgatgcag cagggcggtg ctgttacctg ggccacagcc aagatcggca acgtatttga
     9181 tattctccag cggcactctg gcaagcagct ccaccgccgg acgcgatcgt tcagcggaaa
     9241 agtgcagata taaagagggg ttccagtcag acatttattt actcccgtca gcgcgttacc
     9301 ccaattgtag ataaaattga taaattcgcc tcacggcatc aaaccattga acagacgttt
     9361 tttacgcggc ccggtcatta aagattccag tttcgcgaca caggttttgt agtgaggcgt
     9421 ggttttatgg aatgccactg cgtcttcatc tttataggct tcgtaaatat aaaagcgcga
     9481 attcacttcc gggtcctgta agacatcgaa gcgcaaattg ccttcttcct gtacagagcc
     9541 caggtggttc tggcgaaaaa cttcgataaa ctcgtcaatc ttgtcttcat gaacgttaat
     9601 ttcaaccagt gtgacgtgca taattcttct ccttaaatcc gcagttactg tttttcactc
     9661 agatagagtt catatgcccg atcagccgtt tcgttatggt gaaccaccgc ctgtacggct
     9721 ttcatcatcg ccaccggatg gtcagactgg aaaatattac gccccatatc cacaccagaa
     9781 gcgccctgat cgatagcctg ccagcacatt tccagcgcct cgcgctccgg taatttttta
     9841 ccgccagcaa taacaatggg taccggacat ccggcaacaa tccgttcaaa acctttttcg
     9901 acataatagg ttttgataat ttgcgccccc atttcagcgg cgattcgagt cgcgagcgag
     9961 aaataacgct gatcgcgcac catatctttg cccacgccag tcacggccat ggtcggcatt
    10021 cccactttca ttccggcatc aaccagctga ataatatttt tgatcgactg atgttcatat
    10081 tcgctgccga tataaacctg cgccgccacc gcgcaactgt tcaggcgcac ggcatcatcc
    10141 atcgataacg ccacggcttc attacttaat tccgccagaa tagagttcgc acctgacgcc
    10201 cgcagtacca ccggcttatt ggtcgctggg ggaactacgc tgcgcaaaat gccgcgcgta
    10261 cacattaata catcggcatg ttcaaacagc ggggcgatat ttatatcaat gcgttcaagt
    10321 ccggtagtcg gcccctgaaa ataaccatga tcaaaggcca gcatcacggt attacccgtt
    10381 ttcggattaa atatccgcga taagcgtgac tgcattcccc aatccagcgc accgcaacct
    10441 ttcagggtaa aagggatatt ttgttgcggt tgatcggtac gaaaatcttt accatcttta
    10501 atatcgtcta aatctgccat aacttactcc ggttaagtaa tgcacatcag aaatcgtatt
    10561 tgccgatatt ctctttgttg aatatcacgc gctccggtaa cagtacgatg ccattaccat
    10621 ccgcttcgta gtcatagccc tgaacgctgt ttggcgagac ttcaacctga cctacgccct
    10681 ggatatccag cttgtcgccc gttttcattg atcctttttt caataatgca tccgcgacat
    10741 acactgaaat tttgccttgc tgaaccacat cccacagtcc aaattctttc accgtgccgc
    10801 gctctacata cgggcgcatc acatttggcg tactgaatcc gacaatcgct actttgtcat
    10861 ttttcaggtt ttctgcggct tgtgcggcag cgggcagggc attggcatcg ggggcgataa
    10921 tggcgtcgag atcgctatac gcttttaata ttccttctgc ggtttgtaat gatttagtgg
    10981 catcgttata gccaaactgc gtagtgacaa tttcccagcc aggatgctct ttggcgattt
    11041 tcgctttcgc ttctttcacc cactggtttt ggtccgtaac ggtggggctt gagtagaaaa
    11101 acgcgacttt ggctttgtct ttattcacct gacgcgccgc catatccacc aacatacctc
    11161 ccaactgggc gggcgttccc tgattaatgt agtaagagcg gcactccggt ttagtatcag
    11221 agtcccaggt cagcactctc acaccacgtt gcatggcgcg tttcagtgcc ggacacaagc
    11281 catcaggcga aaccgcagaa acgataatgg cgttataacc ttgattgacg aagttattaa
    11341 tcaactgtac ctgaccagga acactgggtt ctgtcggccc gtcgtaggtc acatcaacgc
    11401 ccagctcttt acccgcttgt tgtgcgccgt tgccaccgct ggtaaaaaat cccacgccaa
    11461 ccagtttggg aataaatgca attacggctg aaaacacgat tatcggtggc aatgccaccg
    11521 gtttcggaac cctgaatgtg caggatcaag actctgtcat cacggtacgc agactctata
    11581 atggatattt cggcaatggc acagtcaata tttccaataa tggactgatt aataacaaag
    11641 aatattcatt ggtgggcgtt caggacggtt cccacggtgt cgtcaacgtg accgataaag
    11701 ggcattggaa tttcctcgga acgggcgaag ctttccgcta tatctatatc ggtgatgctg
    11761 gcgatggtga acttaatgtc tcgagtgaag gcaaagtaga ttcgggaatt atcactgcgg
    11821 ggatgaaaga aacaggcaca ggcaacatta ctgttaagga taagaactcc gttatcacta
    11881 atctcggaac taatcttggt tatgacggcc acggcgaaat gaatatcagt aatgaggggc
    11941 ttgttgtcag caacggagga agctcactcg gttatggaga aaccggcgtc gggaatgtca
    12001 gcatcaccac gggggggatg tgggaggtca ataagaatgt ctataccacc attggtgttg
    12061 cgggcgtcgg aaacctcaat attagcgatg gcggtaagtt tgtatcgcaa aatattactt
    12121 ttttgggcga taaagcaagc ggtatcggca cactgaacct gatggatggg gcatcatcgt
    12181 tcgatactgt gggtatcaat gtcggtaatt ttggtagcgg tatcgtaaat gtcagtaatg
    12241 gtgccaccct taattcaacg ggctatggat ttatcggagg aaatgcctcc ggtaagggga
    12301 tagttaatat ttcaacggat agtctctgga atttaaagac atcatctact aacgcccaat
    12361 tgctacaggt cggtgtatta ggcgcgggtg aactgaatat taccaccgga ggtatagtta
    12421 aagcgcgtga tacacagata gctctcaatg acaaaagtaa gggcgacgtg agggtggatg
    12481 ggcagaactc tcttcttgaa acattcaata tgtacgtagg gacatctggt acgggtacgt
    12541 taaccctgac gaatagcggc acgctgaatg tcgaaggtgg agaagtttac ttaggtgttt
    12601 ttgaacctgc tgtaggaacg ctaaacattg gtgctgctca cggtgaggcg gcggcagatg
    12661 ccgggtttat caccaacgcg acgaaagtag agtttggttc tggcgaaggt gtttttgtct
    12721 ttaatcatac taataacagt gatgccggct accaggtcga tatgctgatt acaggtgacg
    12781 ataaagacgg aaaagtgatc catgatgcag gccatacggt gttcaatgta gggaatactt
    12841 atagcggtaa aacgctggtc aatgacggcc tcctgaccat tgcgtctcat acggcagatg
    12901 gggtaacggg catgggttcg agtgaagtca ccattgcaag ccccggtacg ctcgacattc
    12961 tcgcatcaac gaacagtgca ggagattaca cgctgaccaa tgcgctcaaa ggcgatggtt
    13021 tgatgcgagt gcagctgtca tcctccgaca agatatttgg ctttacccat gcaacaggga
    13081 ctgaattcgc cggtgttgcc caactgaaag acagtacctt cactctggaa cgcgacaaca
    13141 ccgctgcgct tactcacgcg atgttgcagt ctgacagtga aaataccaca tcggtaaaag
    13201 tgggagagca atccattggt ggactggcca tgaatggcgg tacgctcatt ttcgatacgg
    13261 atattcctgc tgcgacgctt gcagagggat atatcagcgt cgatacgctg gttgtcggcg
    13321 cgggtgacta cacctggaaa ggccgtaact atcaggtaaa cgggacgggc gacgtgctta
    13381 tcgacgtgcc taaaccgtgg aatgatccca tggcgaataa ccctctgacg acgctcaatt
    13441 tgctggaaca cgatgatagc catgtcggcg ttcaactggt gaaggcgcaa acggttattg
    13501 ggtcgggtgg ctcattaacg ttacgtgatt tacagggcga cgaggtggaa gcggacaaaa
    13561 cgttacacat tgcgcaaaac ggaacggtgg tcgccgaggg tgattatgga ttccgcctca
    13621 cgaccgcacc aggtgatggt ttgtacgtta actatgggct gaaagcgctg aacatccatg
    13681 gtgggcaaaa gctgacgtta gccgaacatg gcggagccta tggcgcaacg gccgatatgt
    13741 cggcaaaaat cggtggtgaa ggggatctgg caatcaatac ggtgcgacag gtttcgcttt
    13801 ccaacggtca gaacgactat cagggggcaa cctacgttca gatggggaca ttacgtaccg
    13861 atgcggatgg tgcacttggc aacacccggg aactgaacat cagcaacgcg gccatcgtcg
    13921 atcttaatgg atcgacgcag acggtagaga cattcaccgg gcagatgggt tcgactgttt
    13981 tgttcaaaga gggggcgctg acggtaaata aaggtgggat cagtcagggt gaactgacag
    14041 gtggcggaaa cctgaatgtt acagggggaa cgctggctat cgaggggctt aatgcacgct
    14101 acaatgcgtt aaccagcatt agcccaaatg cggaagtcag cctcgataat actcaggggt
    14161 taggcagagg aaatattgcc aatgacggtc tgttaacgct aaaaaacgtg actggcgaac
    14221 tgcgtaatag cataagcggg aagggtatcg tgagcgcaac cgccaggaca gatgtagagc
    14281 tggatggcga taatagccgc tttgtggggc aattcaacat tgatacaggc agcgcgctca
    14341 gcgtcaacga gcagaaaaac ctgggtgatg cttccgttat caataatggc ctgctcacca
    14401 tctccactga gcgtagctgg gcgatgacgc acagtatcag cggtagcggt gatgtgacaa
    14461 aactgggtac cgggatcctg actcttaaca acgattccgc ggcgtatcag ggtacgacgg
    14521 atatcgtggg ggggggggaa attgctttcg gttccgactc tgccattaat atggcaagtc
    14581 aacacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcaggtgatg
    14641 tgaacgttat gcctgggggg acactgcgtg tcgctaaaac cacaatcggc ggcaacctgg
    14701 agaatggcgg cacggttcaa atgaatagcg aaggggggga aaccggggaa tgtactgacc
    14761 gttaacggca actataccgg aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc
    14821 gataattcgc ccaccgataa aatgaacgtg aaaggcgata cccaagggaa cactcgcgtt
    14881 cgggttgata acattggcgg cgtcggtgca caaacggtca acggtattga actcattgag
    14941 gttggcggta attctgcagg taacttcgcg ctgaccaccg gaactgtcga agctggggct
    15001 tacgtctaca cgctggctaa agggaagggg aatgacgaga aaaactggta tctgaccagt
    15061 aaatgggacg gcgtaacgcc agcggataca cccgatccca tcaataatcc ccctgttgtg
    15121 gatccggaag gcccatcagt ttatcgcccg gaggccggaa gctatatcag caacattgcc
    15181 gcagccaact cgctgtttag ccatcgttta cacgaccgtc tgggtgagcc gcagtataca
    15241 gattcactgc attctcaggg gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac
    15301 gaacgttcaa gggccggtga cggtcagcta aatactcagg ctaaccgcta tgtattgcag
    15361 ctaggcggcg atttggcgca gtggagtagc aacgcgcagg atcgctggca tcttggcgtg
    15421 atggcaggtt acgctaatca gcacagtaat actcagagta atcgtgtggg ttataaatcg
    15481 gatgggcgca tcagcggtta cagcgctggg ctgtacgcga cctggtatca gaacgatgcg
    15541 aataagaccg gcgcttatgt tgacagctgg gcgctgtata actggtttga taacagcgtc
    15601 agttccgata accgttctgc tgacgactat gattctcgcg gtgtgacggc ctctgttgag
    15661 ggtgggtata cctttgaagc gggaacattt agcggcagcg aagggacgct gaatacctgg
    15721 tacgtccagc cacaggcgca aatcacctgg atgggtgtga aagattccga ccatacccgg
    15781 aaagacggaa cgcgcattga aacggaaggc gacggaaatg tgcaaacgcg acttggggtg
    15841 aaaacctacc tgaacagcca tcaccagcgt gacgatggta aacagcgtga gttccagcct
    15901 tacattgaag cgaattggat caacaacagc aaagtctacg ccgtgaagat gaatggtcaa
    15961 accgtaggcc gtgaaggtgc gcgtaatctc ggtgaagtac gtaccggggt tgaggcgaaa
    16021 gtaaataaca accttagcct gtgggggaat gtcggtgtgc aactaggtga taaaggctat
    16081 agcgatactc agggcatgct gggagtgaaa tatagctggt aaaccgtata agccgcatgt
    16141 cgagatggca tgcggcttaa tattgccgac ttcaaaggct ttaaagcctc ccttttatcc
    16201 gcgatcgcgg atatcgcagc gtttatcccg tacagcggat aagatgtgtt tccagattga
    16261 ctttatcctc actaaaggat aaaactgata atatcccctt aagcggataa atttgctgtg
    16321 gacgtatgac atgatgagct ttcagaagat ctatagccca acgcaattag cgaatgcaat
    16381 gaaactggtt cgccagcaaa atggctggac gcagagcgag ctggcgaaaa aaattggcat
    16441 taagcaggcg acaatttcca atttcgaaaa caaccctgac aataccacgc tcacgacatt
    16501 ttttaagatt ttacagtcgc ttgaactctc aatgacacta tgcgacacga aaaatgcctc
    16561 gccagaatca acagaacagc aagatctgga gtggtaatgc ctaaacttgt cacttggatg
    16621 aacaaccagc gggtaggcga gttaacgaag ttagccaacg gcgcgcacac ctttaagtat
    16681 gcaccggagt ggttagccag ccgttatgcc agaccgttgt cactttcgct gccattgcag
    16741 agggggaata tcacctcaga tgccgtattt aacttcttcg ataacctgtt acccgatagc
    16801 ccgattgtac gtgaccggat cgttaaacgt tatcatgcta aatccagaca accgtttgat
    16861 ttattgtcag aaatagggcg agacagcgtt ggtgccgtaa cgttattacc cgaaaatgaa
    16921 accataacgc gcccgataat ggcatgggaa aagcttactg aagccaggct tgaagaagta
    16981 ttaacggctt ataaagcaga tatccctctg ggcatgatta gagaagaaaa tgactttcgc
    17041 atctcggttg ctggcgcgca ggagaagaca gcgctgctta gaataggcaa tgactggtgc
    17101 attccgaaag gaataacgcc gacgacgcac atcattaaat taccgattgg cgaaatcagg
    17161 cagcccaatg cgacgctcga tctcagccaa agcgttgata atgagtatta ctgtctgctg
    17221 ctggcgaaag aacttgggtt gaatgttccg gacgcagaaa tcattaaagc gggaagggtg
    17281 cgcgcgttag cggtcgaacg ttttgacagg cgttggaata ctgagcgaac ggttttactt
    17341 cgcttgccac aggaggatat gtgtcagaca ttcggtttac cttcatctgt gaaatatgaa
    17401 tcagatggag gcccaggcat cgcgcggatc atggcgtttt tgatggggtc cagcgaggcg
    17461 cttcgcgatc gttatgattt tatgaaattc caggtcttcc agtggttgat tggcgcaacg
    17521 gatggccatg caaaaaactt ctccgtattt attcaggctg gcggcagtta tcgactcacg
    17581 ccattttacg acatcatttc agcatttccg gtccttggcg gtacgggaat acacatcagc
    17641 gatctcaaac tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa
    17701 atttatccgc gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg
    17761 catgaaatcc taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact
    17821 tcattaccga cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg
    17881 ttgcatggac ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt
    17941 taatgagcac gacaatcatg accgccaacg actggattct tccttggttt atactggtgc
    18001 gcataatgta tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg
    18061 ttattcgttc agggttcaag aacttaagcc tctcctcttt cataattggc ctgtgcatgg
    18121 agaaatagcc tggttattcc ctgctccatg ctaagagcgc agtgtctgtc gcatagacac
    18181 tgcatacgaa aacaccagag ggtgctgtta cttatttaag gacggatcaa gcgggaatga
    18241 gtccactccg gtatcagaaa tggctacgtc tcaatgaagt caggcgaccg atgctgaatg
    18301 aacattacga tgtcaccaca gcagcctatg ctgtcggtta cgaaagctat cccatttcag
    18361 tcgggaatat tcgcggatgt ttggagagtc acccaagaga gatattacca agttaagaaa
    18421 gtctgtcggt cagctttaat aaaccctgca cttatctgtt tagagcaatt cggtgttagt
    18481 ttcagcaagc aaacattaac catagctaat gatttatagc catattaacc attggtagcc
    18541 cttgaccttt cctgacactt cccttgttcg caataatatt ctgttt
//
LOCUS       DAZFAH010000080        18543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFAH010000080 DAZFAH010000000
VERSION     DAZFAH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.080"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..651
                     /gene="yjaZ"
                     /locus_tag="ACHJWB_002606"
     CDS             103..651
                     /gene="yjaZ"
                     /locus_tag="ACHJWB_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO4314216.1"
                     /translation="MYAYKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPL
                     EYRFWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILAL
                     PESNVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGK
                     SVKFFTIAYNLLRNKQLPQPSK"
     gene            complement(1061..2194)
                     /gene="thiH"
                     /locus_tag="ACHJWB_002607"
     CDS             complement(1061..2194)
                     /gene="thiH"
                     /locus_tag="ACHJWB_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4314217.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARKSAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(2191..2961)
                     /gene="thiG"
                     /locus_tag="ACHJWB_002608"
     CDS             complement(2191..2961)
                     /gene="thiG"
                     /locus_tag="ACHJWB_002608"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4314218.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(2963..3163)
                     /gene="thiS"
                     /locus_tag="ACHJWB_002609"
     CDS             complement(2963..3163)
                     /gene="thiS"
                     /locus_tag="ACHJWB_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4314219.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(3147..3902)
                     /gene="thiF"
                     /locus_tag="ACHJWB_002610"
     CDS             complement(3147..3902)
                     /gene="thiF"
                     /locus_tag="ACHJWB_002610"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4314220.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQKINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3895..4530)
                     /gene="thiE"
                     /locus_tag="ACHJWB_002611"
     CDS             complement(3895..4530)
                     /gene="thiE"
                     /locus_tag="ACHJWB_002611"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4314221.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGVSLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(4530..6425)
                     /gene="thiC"
                     /locus_tag="ACHJWB_002612"
     CDS             complement(4530..6425)
                     /gene="thiC"
                     /locus_tag="ACHJWB_002612"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4314222.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(6506..6606)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6658..7134)
                     /gene="rsd"
                     /locus_tag="ACHJWB_002613"
     CDS             complement(6658..7134)
                     /gene="rsd"
                     /locus_tag="ACHJWB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4314223.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            7229..8002
                     /gene="nudC"
                     /locus_tag="ACHJWB_002614"
     CDS             7229..8002
                     /gene="nudC"
                     /locus_tag="ACHJWB_002614"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4314224.1"
                     /translation="MDRIIEKLDHGWWVVSYEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            8042..9106
                     /gene="hemE"
                     /locus_tag="ACHJWB_002615"
     CDS             8042..9106
                     /gene="hemE"
                     /locus_tag="ACHJWB_002615"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4314225.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMIYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            9116..9787
                     /gene="nfi"
                     /locus_tag="ACHJWB_002616"
     CDS             9116..9787
                     /gene="nfi"
                     /locus_tag="ACHJWB_002616"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4314226.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            9830..10420
                     /gene="yjaG"
                     /locus_tag="ACHJWB_002617"
     CDS             9830..10420
                     /gene="yjaG"
                     /locus_tag="ACHJWB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4314227.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10607..10879
                     /gene="hupA"
                     /locus_tag="ACHJWB_002618"
     CDS             10607..10879
                     /gene="hupA"
                     /locus_tag="ACHJWB_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4314228.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            10892..11587
                     /gene="yjaH"
                     /locus_tag="ACHJWB_002619"
     CDS             10892..11587
                     /gene="yjaH"
                     /locus_tag="ACHJWB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4314229.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SNFCRWQPNEKTF"
     gene            complement(11589..12014)
                     /gene="zraP"
                     /locus_tag="ACHJWB_002620"
     CDS             complement(11589..12014)
                     /gene="zraP"
                     /locus_tag="ACHJWB_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4314230.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            12252..13628
                     /gene="zraS"
                     /locus_tag="ACHJWB_002621"
     CDS             12252..13628
                     /gene="zraS"
                     /locus_tag="ACHJWB_002621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4314231.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKERLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IATDQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            13625..14950
                     /gene="zraR"
                     /locus_tag="ACHJWB_002622"
     CDS             13625..14950
                     /gene="zraR"
                     /locus_tag="ACHJWB_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4314232.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(14947..16236)
                     /gene="purD"
                     /locus_tag="ACHJWB_002623"
     CDS             complement(14947..16236)
                     /gene="purD"
                     /locus_tag="ACHJWB_002623"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4314233.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGTPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(16248..17837)
                     /gene="purH"
                     /locus_tag="ACHJWB_002624"
     CDS             complement(16248..17837)
                     /gene="purH"
                     /locus_tag="ACHJWB_002624"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4314234.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            18452..>18543
                     /locus_tag="ACHJWB_002625"
     rRNA            18452..>18543
                     /locus_tag="ACHJWB_002625"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4235 a         4942 c         5051 g         4315 t
ORIGIN      
        1 cgggagtcac atttttctgc atatctgaca ctttggttcc cactacttat ttggtggaca
       61 ccactttgtc taattcgtca gattctgacc agacggttca ggctgtacgc ttacaaacag
      121 gaagtggaaa agtggcgacc ttttggacat ccggatggtg atattcgtga tttatcattt
      181 cttgatgctc atcaggctgt ctacgttcag catcatgagg gcaaagagcc tttagagtat
      241 cgcttttggg ttacctactc tcttcactgc ttcacaaaag attatgaaca tcagacgaac
      301 gaagaaaaac aatcgttaat gtaccacgcg cctaaagaat ctcgtccctt ctgccagcac
      361 cgttataact tagcgcgcac acacttaaaa agaactattt tggcgctgcc agaaagcaac
      421 gttattcatg ccgggtatgg tagctatgcc gtgattgagg tggacttaga cggaggagat
      481 aaggcatttt actttgttgc gttcagggct ttcagggaaa agaaaaaact ccgtttgcat
      541 gtaactagcg cttatcccat ttctgaaaaa cagaaaggta aatcagtgaa atttttcacc
      601 attgcctaca acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
      661 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
      721 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
      781 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
      841 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
      901 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
      961 gctgacggca ctaccataaa aaaacagaac gctttcaaca accttgcgtt tttcatcttc
     1021 cgtagcggct cggaataaat ttacgttctt acattccgtc tcatagtctt tgcgaggccc
     1081 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
     1141 cagcaaccgc ttccggtctg cgatcgtcgt gcggtgagaa ctgttccaac tcggggtgat
     1201 tatcggcata gccacctggc tgcgttttcg agaaggcgct gacgttattg atcgccagcg
     1261 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
     1321 gtgcaagcag tcggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
     1381 caggctcaat gccgccagta cacgggcgca ggcgcggaaa ggagacagag taacggcttt
     1441 gccagtaatg ctgttgcagc catagcaaat gttcggcaac catatagcag tcaacgcgcc
     1501 agttgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacgcccca
     1561 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
     1621 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagct
     1681 gctttaactc ggcgtattcc gtctccgcca gcggttgcac ttccatttgt agtgaagaga
     1741 actgttcacg cagggcgggg agatgacgac gaaagtaatc catccccact ttcgcctgat
     1801 gttcaccagt gactaatagc agatgttcaa agcccatctc ccgtatagcg gcgcttttcc
     1861 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg attactcatg gaaaatccgc
     1921 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
     1981 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
     2041 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgaattgc gaggcattta
     2101 gcgcccgctc tacgtcaaca gccgttttgc cgttgatacg caggcggatg tcatcccagt
     2161 ccagttgtcg ccagcgatcg ctgaaggttt tcatgccgat gcctccagaa atccagtcag
     2221 cgggctggtg gcatgagcaa aatgactgcg gctgcccggt ccggactgac gtgccagcag
     2281 gcctgcttct accgccagac gaaatgcctt cgccatgttg acgggatcgt ccgctacggc
     2341 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agggcctgcg cggcatggct
     2401 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
     2461 cagcatggca caggtttcca gtccctgatt cgagccaatc ggcgcgccga gcggcatcac
     2521 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
     2581 cagcacgaca aatccctgtt gtaccagcat ttcggcggct ttcagagttt cgatgggatc
     2641 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt agccagttag tgcctaacgc
     2701 ttcacgtgcc agatgggcgg cgaaaatggc ttcttccgcc gttttcgccc cggaagtatt
     2761 cggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
     2821 caagtcgaca cgtttcatcg ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc
     2881 catcatcagt tgtgaagaag cgaatttccc tgtgccggta aacagatgtg aatcaaacgt
     2941 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagc aggatctggt
     3001 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
     3061 ccagagccgc gcccgcttgt cgttggtcga gttgctccag tagttcgtga acagtttgcc
     3121 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
     3181 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgattt accgtcgaac
     3241 agtcggagtt ctcccgcagg tgtctctata ccgcttaata acttaatggc ttccagtgcc
     3301 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
     3361 tctggctcct ggttatctgg ccacaggcag cggtaacacc cctgctccca gggcggcgtc
     3421 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
     3481 accacgcagg cggcattaat cttctggcga gtcgccatat tgtcggtaca gtcgagcacc
     3541 acatcggccc gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
     3601 gtcagttgaa tgtcgggatt caactgtgtc agtcgctgtt ggctgacctg cgatttcggg
     3661 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
     3721 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ctaggtatag cgcagcaggt
     3781 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
     3841 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
     3901 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
     3961 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
     4021 tgccagactg acaccgccaa tcgccacggt gggataatcc gccagtcgct caacatgccg
     4081 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
     4141 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
     4201 atgtgtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
     4261 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcca gccgccagta
     4321 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
     4381 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
     4441 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
     4501 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
     4561 ccgcctctgg cacggaagtt ctccgacata tccgccattc ccatttcaat agtttgcgtg
     4621 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
     4681 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
     4741 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
     4801 ttcgacatgg cgttatcgcg aatttgcgcg cccggatgcc ctttcgccag gtcagcggcg
     4861 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
     4921 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
     4981 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
     5041 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
     5101 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacgtcata ttcccaggca
     5161 attttggtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
     5221 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
     5281 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
     5341 catttcgcca taatcgaacc gccgcgagag acgataccgg tcaggcgttt cgcggtcatc
     5401 ggcacatagc gcagcagtac gcccgcatgg atagtgaagt aatccacacc ttgctcggcc
     5461 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccgttaacc
     5521 ttctccagcg cctggtagat cggcactgta ccgatcggca ccgggctgtt acgcaaaatc
     5581 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
     5641 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
     5701 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
     5761 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
     5821 aattccgcag tgatattttc cggcagatgt gcgccaaagc tcattcccgg atgctggtgg
     5881 cgtaaaacct cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
     5941 atttccggcg tgatgatgcc ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgct
     6001 ttggcgcgtt ttggtgttag tacgccgcta aaacgcagtt cgtcgaggcc atcatctgcc
     6061 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
     6121 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc aatctgcgga
     6181 tcaccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
     6241 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
     6301 acgccggggt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
     6361 agggtgtcga taaaatgttg ggcccgggcg cgttgttcgc ggcgggtcag ttttgttgca
     6421 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
     6481 acgatcgccg caggggcgat gcgatagcag attaactctt gttcccttcg caggtattag
     6541 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
     6601 gaaattagcc ccgcaaatgg ggcattgaat gtaaattacg cgttaacagc gcagaactca
     6661 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
     6721 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
     6781 atcatgatcg atggcggttt ccagactgga atcgtagtaa tccataatct gttgagtatt
     6841 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
     6901 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
     6961 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
     7021 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
     7081 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
     7141 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
     7201 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
     7261 ctggtgggtc gtcagctatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
     7321 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
     7381 ggaacctgtt tggttagtac aacagcagcg gcgtcacgat atggggtcgg tacgtcaggt
     7441 cattgatctc gatgttgggc tgtttcaact ggccggacga ggcgtacaac tggcggagtt
     7501 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccga gcaaaaccga
     7561 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
     7621 tattgttgcc atccgtcgcg atgattcgat cctcctcgct cagcataccc gccatcgtaa
     7681 cggtgtccat acagtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcagt
     7741 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa aacttgcgtt acgtgacttc
     7801 tcagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
     7861 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
     7921 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
     7981 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
     8041 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
     8101 cactccagta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
     8161 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gcgaagtgac
     8221 tttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
     8281 cgtgccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
     8341 ctcgccagtc acctgcaaag ccgacgtcga taaactgcca attccggacc cggaagatga
     8401 gctgggttac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
     8461 gctgattggt ttttccggca gcccgtggac gctggcaacc tacatggtgg aaggcggcag
     8521 cagcaaagcc ttcaccgtga tcaaaaaaat gatatatgcc gatccgcagg cgctgcacgc
     8581 tctactcgat aaactggcga aaagcgtcac tttgtatctg aatgcgcaga ttaaagccgg
     8641 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
     8701 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
     8761 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
     8821 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
     8881 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
     8941 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
     9001 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
     9061 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
     9121 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
     9181 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
     9241 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
     9301 taaagtggcc cgcatcgcca ccaccatgcc ttacattcca ggttttcttt ccttccgcga
     9361 atatcctgcg ctcctggcag cgtgggagat gctgtcgcaa aagccggatt tagtgtttgt
     9421 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgat
     9481 ggtggatgtg ccaacgatcg gcgtggcgaa aaaacggctc tgcggtaaat tcgaaccgct
     9541 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
     9601 ctggcgcagc aaagcgcgct gtaacccgtt gtttatcgct accggccatc gggtcagcgt
     9661 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
     9721 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
     9781 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
     9841 cccaattcat ctgcgtctgg agcgcctgga aagctggcag catgtcactt tcatggcttg
     9901 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
     9961 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagacgc
    10021 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    10081 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    10141 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    10201 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    10261 taaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    10321 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    10381 ggcgggtgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    10441 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    10501 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    10561 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    10621 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    10681 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    10741 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    10801 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    10861 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    10921 accccttttg tctttctggc gtcgatcatt gatgctggca ggtgcgctgt ttctcactgc
    10981 ctgtagccac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    11041 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg catctgcttg ccgtgtttag
    11101 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    11161 gctcatcaat atcaatgttt acagcaaacc gccggtgaat attcgcgcgc gttttgacga
    11221 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    11281 cgaccaaatc gatttatacc gttatcgtgc tgatcagatc cgccagatta gcgatgcctt
    11341 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    11401 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcgatag cgcatatcga
    11461 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    11521 ttcgcaatta ctgttagtgg caaactctaa tttctgtcgc tggcaaccca acgagaaaac
    11581 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    11641 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcgatatc tcgtttcacc
    11701 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    11761 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    11821 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    11881 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    11941 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    12001 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    12061 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    12121 tcgcctgctg cggatagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    12181 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    12241 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    12301 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    12361 attatgggcg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    12421 ttcgcgctct tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    12481 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    12541 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    12601 cccctcagga aatgcagcag ttacatccgg gagatgaaga agcgtggcgg cggatcgata
    12661 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    12721 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    12781 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    12841 ttctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    12901 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aggctggtgg
    12961 cgctggggca tcttgcggca ggcgttgccc acgaaatccg taacccactt tcctcgatta
    13021 aaggactggc gaaatacttt gccgagcgcg cgcctgcagg gggagaagcg catcaactgg
    13081 cgcaggtgat ggcgaaagag gccgaccgtt taaaccgcgt ggtaagcgag ttgctggaac
    13141 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    13201 cattacagct ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca
    13261 acgacacatt accggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    13321 tctatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggtcagcg
    13381 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcgacagatc
    13441 agcttgaagc catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc
    13501 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg
    13561 agggaaaagg ctcaacgttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    13621 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    13681 tattttgcag gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    13741 acaggcgctg gagcaggtgc gggaacaggt ttttgatctt gtgctttgcg atgtgcgaat
    13801 ggcggagatg gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc cggcaattcc
    13861 ggtgctgatt atgactgcgt actccagcgt cgagacggcg gtagaggcac tgaaaactgg
    13921 ggcgctggat tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa
    13981 agcgctcgcg catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt
    14041 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    14101 gccatcggaa gccacggtac tgatccacgg cgattccggc accggtaaag agctggtcgc
    14161 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    14221 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    14281 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct
    14341 cgatgaaatt ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga
    14401 gcgcgaagtt cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc
    14461 ggcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    14521 ctatcgcctg aatgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    14581 ccctctgctg gctggccatt ttctgcagcg ctttgccgag cgtaatcgca aggcggtaaa
    14641 aggttttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    14701 tgagctggaa aacgcggtgg aacgggcagt ggtgctgctg accggggaat atatttccga
    14761 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctgggacaaa gtcaggatat
    14821 tcagccgttg gtggaagtgg aaaaagaggt gattctggcg gcgctggaga aaacgggcgg
    14881 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctat tggcaaaact
    14941 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    15001 gtcgtcccag tgaatatcgg tcattaaggc ataagcgcgt ttctgcgctt ctgccacggt
    15061 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gtcactacct gctcgtcatc
    15121 cgccagtttt gtgcctgcat ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    15181 gtggatcaca tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    15241 gagagaagcg cgttcatccc actcggatgt tttctcgtcc agcttccctt cacaggctgc
    15301 caggcagagc tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc
    15361 gccaaagcga cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc
    15421 cgcgtagaga aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca
    15481 gatgatacgt tccatggtgc gctgatgaac ttcatcggtt actaccggcg cgggggagta
    15541 agcgcccatc ccgcccgtgt ttggtccggt atctttatcg cctacgcgtt tgtgatcctg
    15601 gctggtggcc atcggcaaca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    15661 gccatcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc
    15721 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    15781 cccggcagcc agaccgtccg ctttaatgac gattggcgtg cctttctcac gcagatacgc
    15841 cagcgcaggt tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc
    15901 caggaaatct ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc
    15961 gaagattttc agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    16021 cgggccgacg atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat
    16081 atcggtcacg ccaatagcaa cgttttgcag cgcgggttcc agcgcagtgc ctgcattacc
    16141 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    16201 ctcgcgcccg ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag
    16261 tggcgcatgt cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    16321 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    16381 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    16441 aggccttcat cagcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    16501 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    16561 aacttcgcca ctttccagca gaacagcgca tcgcgcagtt cctgttcggt cggctgacgt
    16621 tgggtgacga cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc
    16681 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    16741 gtcagaacgc gtacgttctg cttggcggcg gtgattttca gggcttcttc gctggcggaa
    16801 ggcgcaataa tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    16861 agctcgcggt taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg
    16921 cgatcgtaag catcaagaat ggaattgccg atagctacac cgcaagggtt ggcgtgcttc
    16981 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    17041 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    17101 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    17161 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    17221 ttgctttcac cgtggtacgc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    17281 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    17341 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    17401 attgcgacat ctttatggtt cttagcggcg gagcgcacca tggtcgggcc gccgatatcg
    17461 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    17521 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    17581 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    17641 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    17701 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    17761 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    17821 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    17881 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    17941 tgtaacgaaa acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt
    18001 ttgacagtgg ttaccgtggg aaaaatacag aaattacatt gatgattgtg gataactctg
    18061 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    18121 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    18181 atgtgaatta cttcacacaa acagccggtt cggttgaaga gaaaaatcct gaaattcagg
    18241 gttgactctg aaagaggaaa gcgtaatata cgccacctcg cgacagtgcg ctaaagcgcg
    18301 tcgcaactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    18361 cttaacgtcg caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta
    18421 cgaagtttaa ttctttgagc atcaaacttt taaattgaag agtttgatca tggctcagat
    18481 tgaacgctgg cggcaggcct aacacatgca agtcgaacgg taacaggaag cagcttgctg
    18541 ctt
//
LOCUS       DAZFAH010000081        18482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFAH010000081 DAZFAH010000000
VERSION     DAZFAH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.081"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..1301
                     /locus_tag="ACHJWB_002626"
     CDS             81..1301
                     /locus_tag="ACHJWB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4314235.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1274..1903
                     /locus_tag="ACHJWB_002627"
     CDS             1274..1903
                     /locus_tag="ACHJWB_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4314236.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGQELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1904..3064)
                     /gene="ybdL"
                     /locus_tag="ACHJWB_002628"
     CDS             complement(1904..3064)
                     /gene="ybdL"
                     /locus_tag="ACHJWB_002628"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4314237.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3172..4260
                     /gene="hcxA"
                     /locus_tag="ACHJWB_002629"
     CDS             3172..4260
                     /gene="hcxA"
                     /locus_tag="ACHJWB_002629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4314238.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWICGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4270..4467)
                     /locus_tag="ACHJWB_002630"
     CDS             complement(4270..4467)
                     /locus_tag="ACHJWB_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4314239.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4649..6754)
                     /gene="cstA"
                     /locus_tag="ACHJWB_002631"
     CDS             complement(4649..6754)
                     /gene="cstA"
                     /locus_tag="ACHJWB_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4314240.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6935..7348)
                     /gene="entH"
                     /locus_tag="ACHJWB_002632"
     CDS             complement(6935..7348)
                     /gene="entH"
                     /locus_tag="ACHJWB_002632"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4314241.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7351..8097)
                     /gene="entA"
                     /locus_tag="ACHJWB_002633"
     CDS             complement(7351..8097)
                     /gene="entA"
                     /locus_tag="ACHJWB_002633"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4314242.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8097..8954)
                     /gene="entB"
                     /locus_tag="ACHJWB_002634"
     CDS             complement(8097..8954)
                     /gene="entB"
                     /locus_tag="ACHJWB_002634"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4314243.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTSDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8968..10578)
                     /gene="entE"
                     /locus_tag="ACHJWB_002635"
     CDS             complement(8968..10578)
                     /gene="entE"
                     /locus_tag="ACHJWB_002635"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4314244.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10588..11763)
                     /gene="entC"
                     /locus_tag="ACHJWB_002636"
     CDS             complement(10588..11763)
                     /gene="entC"
                     /locus_tag="ACHJWB_002636"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4314245.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12138..13094
                     /gene="fepB"
                     /locus_tag="ACHJWB_002637"
     CDS             12138..13094
                     /gene="fepB"
                     /locus_tag="ACHJWB_002637"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4314246.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13098..14348)
                     /gene="entS"
                     /locus_tag="ACHJWB_002638"
     CDS             complement(13098..14348)
                     /gene="entS"
                     /locus_tag="ACHJWB_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4314247.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14459..15463
                     /gene="fepD"
                     /locus_tag="ACHJWB_002639"
     CDS             14459..15463
                     /gene="fepD"
                     /locus_tag="ACHJWB_002639"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4314248.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15460..16452
                     /gene="fepG"
                     /locus_tag="ACHJWB_002640"
     CDS             15460..16452
                     /gene="fepG"
                     /locus_tag="ACHJWB_002640"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4314249.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16449..17264
                     /gene="fepC"
                     /locus_tag="ACHJWB_002641"
     CDS             16449..17264
                     /gene="fepC"
                     /locus_tag="ACHJWB_002641"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4314250.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17261..18394)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJWB_002642"
     CDS             complement(17261..18394)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJWB_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4314251.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4328 a         5240 c         4785 g         4129 t
ORIGIN      
        1 actctgcttt gcagtgttta ctttataatt aacagactta tctcactgat caccctgtaa
       61 cgttcagaga gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg
      121 aagctgcacg agagcgtatt acctggacac tcaacaccct accccgcgtt tgtgtctcat
      181 tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa
      241 tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc gcaattctct tgcaccatta
      301 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
      361 cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg
      421 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat cctgactttt
      481 tctgctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt
      541 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
      601 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa ccatggacta
      661 cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg
      721 atatctggac ctggtatgca aatcatcaaa atctctgcaa cccactgtat aacctaatgt
      781 atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
      841 gacaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct atgtgcgcac
      901 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgataatcat ttctatgggc
      961 atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta ttgctgctca
     1021 atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact
     1081 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
     1141 atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg
     1201 cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
     1261 gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt
     1321 tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc gcatggcgat
     1381 ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg ttaaaaacag
     1441 ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct
     1501 gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa
     1561 aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc
     1621 attaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca
     1681 acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgccatcaaa tcaccgccat
     1741 gtcggaaatc gtccgtgaac ttagccagtt gggatgggac gataataaaa ttggccaaga
     1801 gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa attaacggcc tgcaagagct
     1861 gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa taactaaagc tggcgcaggc
     1921 gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat
     1981 gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc cgctacgccg tgctcctgcg
     2041 tcagccactg gcaaaactca acatcatcca gggtagatac ggcgctgtaa tccaccagca
     2101 aaaagtatgt accttcacac ggtaaaatct ccagccggct ttcatttaaa gcattcacca
     2161 gaatatcgcg cttctggcga taaaagtccg gtaacgcaag ataatgctca ggttctgcac
     2221 gtagcatatc agcaagcgcc agctgtgccg gggtattcac cgaaaaggtc agatactgat
     2281 gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac acaataaccc actttccagc
     2341 cggtcatatg ataggtcttg ccaaatgaag aaaccgccac tgcccgctca cgcagctgcg
     2401 gatgcgccag cacactggca tggccctgtt gtgaaaagtt gatgtgctcg tagacttcat
     2461 cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg aaatcagcct
     2521 gctgccagac agttgcactg ggattatgcg gagtgttgag gatcaccagt ctggtgcgct
     2581 cgcttaacaa tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt tgcagtgcca
     2641 tacgcttcac tattccccca gaaagcgcga tggcgggggc gtaactgtca tagctgggat
     2701 caaaacaaat cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg tataacgctt
     2761 ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc tggttgatag ccatacaaac
     2821 gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg ggcgcgtatt
     2881 ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc ggaccatcaa
     2941 aatcaggaaa gccttgcgac aggttaatcg cctggtgttg ctgcgccagc gcgctcatct
     3001 gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg
     3061 tcatttttta ttgtgcctga cgaagttgtg ttgaaggcac tataacacga tgccagtatt
     3121 tggcaatcaa gacgtttaga tgtctaaata aaacaataag gacaacacaa catgcctcac
     3181 aatcctatcc gcgtggtcgt cggcccggct aactactttt cacatccagg aagtttcaat
     3241 cacctgcacg attttttcac tgatgaacaa ctttctcgcg cggtgtggat ctgcggcgaa
     3301 cgcgccattg ctgcggcgca aaccaaactt ccgccagcgt ttgaactgcc aggggcaaag
     3361 catattttgt ttcgtggtca ttgcagcgaa agcgatgtac agcaactggc ggctgagtcc
     3421 ggtgacgatc gcagcgtggt gattggcgtc ggcggcggcg cactgctcga caccgcgaaa
     3481 gccctcgccc gccgtctcgg tctgccattt gttgccgttc cgacgatcgc cgctacctgc
     3541 gccgcctgga caccgctctc cgtctggtat aacgatgccg gacaggcgct gcattatgag
     3601 attttcgacg acgccaattt tatggtgctg gtggaaccag agattatcct caacgcgccg
     3661 caacaatatc tgctggcggg gatcggtgac acgctggcga aatggtatga agcggtggtg
     3721 ctggctccgc aaccagaaac gttgccgcta accgtgcgac tggggatcaa taatgcgcag
     3781 gccattcgcg acgtattgtt aaacagtagc gaacaggcgc tgagcgatca gcaaaatcaa
     3841 cagttaacgc aatcattttg cgatgtggtg gatgctatta ttgctggtgg tgggatggtc
     3901 ggtggtctgg gcgatcgttt tacgcgtgtg gcggcggctc atgccgtgca taacggtctg
     3961 actgtcctgc cgcaaaccga gaaatttctc cacggcacca aagtcgctta tggcattctg
     4021 gtacaaagcg ccttgctggg tcaggatgat gtgctggcgc aattaaccgg ggcgtatcag
     4081 cgttttcatc tgccgaccac gctggcggag ctggaagtgg atatcaataa tcatgcggag
     4141 atcgacaaag tgattaccca caccctgcgt ccggtggaat ccattcatta cctgccagtc
     4201 acgctgacac cagatacgtt gcgtgcagcg ttcgaaaaag tggaatcgtt taaagcctga
     4261 cgtgcaaatt tagcagcagc gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc
     4321 acggaaaaac tcttcgtagg tcatcggcgt ttgatcggga tggttaaccc gcatatgttc
     4381 gacatagttg tcgtaatcag gcataccaat catcagcttc gccgcctgac ctaaatattt
     4441 tccggctttt gccagtgaat caaacatctt ggcctccgct aaccatgttg ctgaaagagt
     4501 gccggatgcg gcgtgaacgc cttatccggc ctacaaatgc tatcaacgcg ctcggtcagt
     4561 cccctcgccc catcggggag agggttaggg tgaggaggaa ccctcggcac tatgccaaca
     4621 ttcgccaaca tcccccctca ctctgacttt agtgtgcgcc ttttgcctgc gccacgatct
     4681 cctcgacatt ttccggcatt ggctcatacg gcgtttcttt cgccgttggc ttcggatctt
     4741 tcaatgccgc cagtgccgtc ttaatcgaga acagcgccag aaccaccacg accaccataa
     4801 agaagatggt taacccggca tccagacggt tgttgaacac cagttgcgcc agctgtgact
     4861 cagtatactg cgacggaata ttgccgctgt cgatcattgc ctggaactta ttagcaatgg
     4921 ccaggaagcc gactttcgca tccgggctaa acgctttctg ccagcctgcg gtcagggtac
     4981 aaatcagcag ccaggccgtt ggtaccagcg ccacccaggc gtaacgttga cgtttcatct
     5041 tgaacaacac cacggcacag agcatcagcg ccatccctgc cagcatctgg ttggcaatac
     5101 caaacagcgg ccacagagtg ttaatgccgc ccagcggatc gaccacgccc tgatggagga
     5161 agtagcccca cgccagtacg cacagcgctg ttgccagcag gttagcaggc agtgaatcgg
     5221 tccgcttcag gccaggagac accacgccca gcagatcctg caacataaag cgcgcagcac
     5281 gcgtacccgc atctaccgcc gtcagaataa acagagcttc aaacagaatg gcgaagtgat
     5341 accagaacgc cacatccatc ataccaccca gcgcaccgtg caggatgtaa gccatcccca
     5401 ccgccagcgt cggcgcaccg cccgcacggg aaatgatcga ctgttcaccc acttcgctgg
     5461 caatctggtt gagcgtatct ggtgtaatgc taaagcccca gctactcacc acctgcgcgg
     5521 cagaagcgac tacatccgcc gtccctgccg gagccagcac cgccattggg ctgttcatgg
     5581 caaaatacac gcccggatcg atgatacagg cagaaaccag cgccataatc gccacgaagg
     5641 attccatcaa catcccaccg tagccgataa agcacgcctg cccttcgttc gccagcatct
     5701 tcggcgtggt gccggaagag atcagcgcat ggaagccaga caccgcacca caggcgatgg
     5761 tgataaacag gaacgggaac aggttaccgg tccataccgg gccagtgcca tcgacaaatt
     5821 tggtcagcgc aggcatggtc agcgtcgggc gcataatcaa aatgcctacc gccagaccga
     5881 cgatagtccc gattttcagg aaggtcgaga gatagtcacg cggggccagc agcaaccaca
     5941 ccggcagcac cgccgccaca aaaccgtagc ccaccagcat ccaggtcagc tgcacgccgg
     6001 taaagtcaaa gtacggtgcc caggtcggac tttctgccac ccagccgcca gagataatgg
     6061 cgaaaatcag gaacaccagg ccaatcactg acacttcgcc aatgcggccc ggacgcagat
     6121 agcgcaggta gattcccata aaaatcgcca gcggaatggt gaacgcaacg gtatacgttc
     6181 cccacgggct atgagtcagg gctttcacca cgatcatcgc cagtactgca aggataatga
     6241 ccatgatcat aaagcaggct accagcgcaa tcaccccggc agttggcccc atctcttctt
     6301 tgaccagctc acccagcgat cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat
     6361 cctgcaccgc accagcgaga accaccccgg cgagcagcca gatcatccct ggcaggtaac
     6421 ccatttgcgc cgccagtacc ggcccaacca acggacctgc tccggcaatg gccgcaaaat
     6481 ggtgaccgaa cagcactttc ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca
     6541 ccgctggcgt catacgcgtc gggtcaaccg ccagcacatt tttggcgata tacagcccat
     6601 aaaaacggta agcgatcaga tagatacaga ccgacgccac cacaatccac agcgcgttga
     6661 tctgttcccc acgatttaaa gcaatgtatc ccagagcaaa tgctcccatt acagagagca
     6721 ctgtccagac gaggtatttc cctgatttgt tcatagttgt tatccgtgtg cgtgtccata
     6781 gagatgttac attttgtttc tataacatct ttgtacgatt taacaacacg gtaaccatgt
     6841 aaatcggagt tgaaaccaat atttaactta acaatgttaa ctcgatcact ccgttaaccg
     6901 agttttcctg caatctcatt taattctgtc gggctcatcc caaaactgcc gtacccagcc
     6961 gacaagtgca gcaacgccgc ccctgttcat cgaaaacgac gatttcccag ctctgatttt
     7021 gccgcccaag atgcagcggc tggcagacgc cgcgtacctt gccttcagac accgggcgat
     7081 gatgggtggc gttaagttcc gtacccacca cacactgccc gtcacgggtc atcataaatc
     7141 cggccatcga tcccagcgtt tcagccagcg ccgctgacgc gccgccatgc agcaggccaa
     7201 acggctgatg agtacgggta tcaaccggca tttcggcttc cagcacatca tctcccagac
     7261 gggtatacac aattcccaga tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt
     7321 cgagcgttaa atggcgtttc cagatcatgc ttatgccccc agcgttgagc cgccatcgac
     7381 cacaatatcc tgtagggtga tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt
     7441 ggcgatctct tgtggacggg cgattttccc cagcggaatg ccgagtttaa actgctcgcc
     7501 aaagccgcga atacgctgtt cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc
     7561 ggtgtcggtg gagccaggcg aaaccacatt acagcgcacg ccgctaaccg ccagttccag
     7621 cccgacgctc aacgccaggc ttttcagcgc cgctttcgat gcgccataag cactcatacc
     7681 aatacgcggc gtgtgtgcgg cgtcggacgc cacagtgaca atcgccccgc cccgctgacg
     7741 gcgaaactgg ttcatggttt gctgaaacag gttaaacgca ccgccgacgt taaccgcaaa
     7801 agtctgctgc cagtcctctt tactgagctg atcggtcgcg cccatgcgca aaattcccgc
     7861 cgcattgacc agcacgtcca gtcgctccgt ttcagctaac agtcgctgac atacctgcgc
     7921 gacctgcgca gcatcggcaa catccatcac ttcggtcgca aagggatatt gctcctgagc
     7981 gaacgcttga tcaaaaccgg taactttcgc tcccgcttca acaaacgcca gcgccgtggc
     8041 gtagccgata cctttacctg cgccggttac ccagacattt ttaccgctga aatccattat
     8101 ttcacctcgc gggagagtag cttccaccat gcgtcgatgg tcgggttttt cgccagcatg
     8161 acaaagtcga tatcgccatg cactttgcgc cagcgcgccg ccagcgccat catgcgcacc
     8221 gaatccagac cgtagtcgat caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc
     8281 aacggcagga tcacctcacg cagcgccgct ttgctggcgg ggataggtgc tggcagcaat
     8341 tcttcggtca tcaccacccg gccagaacgt ccggccacat atttcagcga catcaaatgc
     8401 tcgtcacggc tgaaatcggc cagcgcatct gccaccataa acggtttaat atcgcgcata
     8461 aatgcgtcgg ttgcggtggt catacaacca atgtgggcat ataccccggt aataatcagc
     8521 tggttacgtc cactctcttt gagcatttgc tccagcggag aacgatgaaa cgcgctgtag
     8581 cgccacttca ccagcaccgt gtcgtcggca tctgacgtca ggcgatccac caccttttgc
     8641 tgttccggcg aacgggtcag gcctggcccc cacatatcat tcaacagcgc ccgatcttca
     8701 tcgctctgct ctttcggctg ggcggtgtaa taaaccggga tattgtgctg tttgcagtag
     8761 tcgcgtagtg ccgcaatatt cgcgatcact tgctccatca tcgggcagtt ctcgccccag
     8821 aagctgacaa aatagtcctg catatcatgg attaacaacg cggcacgttg cggttcaaag
     8881 gcccagtcga ctttattctg cggaatatcg tgagactccg gcagtgcgta agcctgtaat
     8941 tttggaatag ccatcgtgtt ctctccttca ggctgatgcg cgtgacgcca gccactgacg
     9001 taattgtttt ttatcgactt tcccgaccgc cgtcagcgga agtgaatcca cacactccac
     9061 gcgatccggt aatttaaatt cggcaatacc ctgttcacgc aggaaacgac gcacctgcac
     9121 cgcgcgcagc ggctctttta ccaccagata agcgcagctt ttttcgccca tcagctcatc
     9181 ttccatgctc accagtgcgg catagatcac tgccggatgg cgcagcagca ggttttcgat
     9241 ctcttcggca gcgatcttct cgccgccacg gttgatctga tctttctcgc gcccctgcac
     9301 ggtgatgtaa ccctctggat caatagagat cagatcgccg gaacagtaaa aaccgttggc
     9361 atcaaaggca ctggcattgt gctgcggact tttgtaatag ccacggaagg tgtacggccc
     9421 gcgcgtcatc aggcgtccaa cttccccttg cggcagtgga tttccttcgg catcggcaac
     9481 ccacacttca tcgtccggac acattgggta accctgggta tggataattt tctccgcgct
     9541 atcatcaaga cgggtgtagt tcaccagccc ttccgccatg ccaaacacct gctgcaactg
     9601 gcagccaatc tcagcgggaa tacgcgccgc aagggtggca gaaagacgtg cgccgccgac
     9661 ctgtaacagt ttcagcgagg caagctgcgc ccggctttca ccttcggtca gcgcctgcaa
     9721 ccacaggctg actgcgggtg gcaccagcgc ggtgacgttc acctgatgtt tttcaatcaa
     9781 tgggaagcaa agcgtggcgc tgggatcggc cgccagaaca accgttcctc cggcaagaaa
     9841 gacgcccagc gatcccggcg aactcatggc gtagttatga gccgccggga tcgcgcacag
     9901 gtagcgtgtc tgttgtgtga actgacaaat ctcgacgcta cgacgcacgc tgtagtagta
     9961 gtcgttatga gtgcgcggga tcagtttcgg tgtgccggtg gtgccgccgg aaagctggaa
    10021 ataggccact tcatcagcag gtgatggcgt ggcagtaaaa tcctcagccg gatggttaat
    10081 cgcatcctgc aagttatgct caccgctgtc gttgtgcagt tgcaccacgc gaatggagga
    10141 atgttctgtg acgaaagtat tgaggaaatc atccccgcta aacagcgcat gttggcgatc
    10201 ggcaatcagt aacgccggtt caatctggct ggcataggcg ttcagttcac tacgctgatg
    10261 gctgaacagc gccagcaccg gcgcaacgcc cagtttcagc agcgcgaaaa aggtaatata
    10321 caattcagcg acgttaccca gttgtaccag cgcggtttca ccaggtttaa tgccctgacg
    10381 gcgtaaacta cacgcgaggt tatccgccgc ctgattcagc tcccggtaac tcaactgtcg
    10441 ctcgccgtcg ataaccgcga tgctgtcact cgcagcgtgg cgagtcagaa tgtcggtcag
    10501 cggcaaatcc tgccagtagc ctttttcccg atagcgacgg gcaaactctt ccggccagcg
    10561 ggtgaatgga atgctcatcc tcgctcctta atgcaatcca aaaacgttca acatggtaga
    10621 aagtttgacg cctgtttcgc gccactcacc caacggtgac gacgcaggca caatccccgc
    10681 tccggcaaac agacgcacct gattttcccg cagcttcgcg cagcggatgg tcaccaccca
    10741 ttcgccgtta ccttcgctgt cacaccaacc cacaatgccg ccaaacagtt cgcggtcgaa
    10801 tggctccagt tcagcaataa cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg
    10861 ggttggatgc agcagacagg ccagagtcag tgcgttttct tgcgaattcg ctttaccttc
    10921 aaagggagtt gcgagatgcc acagcgtcgg cgtggtaatc aattgtggag aggagggaac
    10981 gtgtaactca ctactgcgtt cgcgcagtac ctctttcatc gcctgagtca ccagttcatg
    11041 ttcatggcga tctttttctg acgccagcag acgattaccc gcttcgcgat cgagcacttc
    11101 atccggctga cgacgcgcgg aaccggctaa cggaatggag ctaaaacgct cgccgtcttt
    11161 acgtagcagc agttccgggc tggcccccag caggacgcca ccatcagcca gcgggacatg
    11221 gaagttgtaa ctaaccgggt tttgcgcaat caaccgttcc agcaatacgc cactatcaat
    11281 ggcggcgtca gtggtgatat caatcaatcg tgacaacacc actttgtcga cctgcggcgt
    11341 ggcggtaagt gcggcggcgc gggcaaccat ctgttcaaac gtggtttgtt caggaattgc
    11401 ctggcgttcc accacgttca gcgactggct gcgggtaaaa cggcgggctg aggtctgttt
    11461 ttcctggcgg gagaacgact gccaggattc gggaatatac agcgacgaag gctgacgtgg
    11521 atcgaaggga atcgccccga ccatcaccgg atttttgatg ccctgcgctt tggcatcggc
    11581 aaacagcgcg gcgagttttt gctggaaggg actgtcgggc gaatccccgt tcacagccgg
    11641 ttcatcgaag cgggcgaaac atcctgacgt cgtaaaactg cggtacggcg acataaagaa
    11701 aaagcgattg ggcgcaagtg ttgccatggt ctgctgtact tcctcagcca gtgacgtatc
    11761 catatcatcc tccacaaaat gataaaggct ttaataatga ttatcattta tattttcggt
    11821 cgctaaccta aaagcaaacg cgcactatgt caactcttga ggtaacgcaa tctgcaactc
    11881 ggggttgcat ctggttgtgg ttacaatgaa aatgagaagc attattgatg gattcgcata
    11941 agcgcaatgt gatgtcctgc gccgttctgc cccctctccc ttccagggtg agggctgggg
    12001 tgagggttaa tgttcgcacc agtgctggct gttcccctca ccctaaccct ctccccaaag
    12061 gggcgagggg acggattgtg cgctttgtcg aatttgtcat tacgccctta accttattaa
    12121 taacaggaag ctgatttgtg agactcgccc cgctctaccg caacgccctt ctattaacag
    12181 gacttttgct ttcaggaata gccgcagttc aggccgctga ctggccgcgt cagattactg
    12241 acagccgtgg cacacataca ctggaaagcc agccgcagcg tattgtttcc accagcgtca
    12301 ccctgaccgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc
    12361 cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag
    12421 aacgcaaact gcaacggctc tatatcggcg aaccgagcgc cgaagccgtt gccgcgcaaa
    12481 tgccggatct gattttaatt agcgcaaccg gcggggattc ggcgctggca ctgtatgatc
    12541 agctttccac catcgccccg acattaatca tcaattacga cgacaaaagc tggcagtcgc
    12601 tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac
    12661 agtttgataa gcaactggca gcggcgaaag agcaaatcaa attaccgccg cagccggtca
    12721 ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac
    12781 aagggcagat gctggaacaa ctcggcttta cgctggcgaa gttgcccgca gggttaaacg
    12841 ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa aatctggctg
    12901 cggggttaaa cggtgaatcg ctattcctgt tcgccggtga tcagaaagac gccgatgcga
    12961 tttatgccaa tccactgctc gcgcacctgc ccgcagtaca aaacaagcag gtttatgcgc
    13021 tgggaaccga gacgttccgt ctggattact acagcgccac gcaagtgctg gaacgactta
    13081 aggcgctgtt ttaagcatta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13141 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13201 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccag cagcgccgcg
    13261 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13321 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13381 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13441 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13501 tgtgccagct tcccgctggt taacgcacca atagccgcgc cgagcgggat cgccgcgtag
    13561 agaaaaccaa tctgtgccgc tgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13621 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13681 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13741 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13801 gccgccagcc cgtagttcca ggctacgcca ccggtcgcca gcaataaacc gccaatcatg
    13861 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13921 ttttcacgcc ctaccagtgc tggtgtcgcc gccaatagcg ccgtaacgcc aagcgatgcg
    13981 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagt
    14041 gcattaaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14101 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14161 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14221 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14281 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14341 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14401 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14461 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14521 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct
    14581 ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14641 gccgcgcacc cttgccggtt tacttgcagg cggcgcgctt ggccttgccg gggcgttgat
    14701 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga atgccggagc
    14761 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14821 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta ccggcagtca
    14881 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    14941 gctggaagga ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15001 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15061 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15121 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15181 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15241 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15301 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15361 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15421 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15481 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15541 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15601 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15661 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15721 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15781 atggtgctgt ttggtcagga cctgacggct atcgccctgg cagcaatggt gggcggcatt
    15841 gtcacttcgc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15901 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg
    15961 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgcaggatc gctcaacggc
    16021 ctgacgtggg caaaaacctc gccttccgca cccatcatta tattgatgct cattgccgcc
    16081 gccttactgg tacgccggat gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg
    16141 ggcgtcagcg tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16201 gcggcaaccg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacatttcc
    16261 cgacgcatta gcggcaccgc tcgctggggg ctaacccagg cggcgctatg cggggcgctg
    16321 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16381 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16441 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16501 gcaaatatac cgttgcggag aatctgacag tagaaatacc tgatggtcac ttcacggcaa
    16561 ttatcgggcc aaatggctgc ggtaaatcca ctttactgcg taccttaagc cgcctgatga
    16621 cgcctgctca cgggcatgtc tggctggatg gcgagcacat ccaacattac gccagtaaag
    16681 aggttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16741 tgcaggagct ggtggcgcgt gggcgttacc cgcatcaacc gctgtttacc cgctggcgca
    16801 aagaagatga agaagcggta acgaaagcga tgcaggccac gggaataact catctggcgg
    16861 atcaaagcgt ggataccctt tctggcggac aacgccagcg cgcgtggatc gcgatggtgc
    16921 tggcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    16981 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17041 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17101 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17161 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17221 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17281 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17341 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17401 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgatat
    17461 ttgcttttgt taactgctca acaagatact gacgattacg taattcaccg ttcagttccg
    17521 caacgtcagt gaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17581 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17641 ttcccgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17701 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgattt
    17761 ccagtttatt acggacgttt tctagtgact ctttcaccac caacgcagag atataatcga
    17821 tatacccgct caaaacggtc tgcgcctctt cactggttgg cgcggtaaaa cttagcgtcc
    17881 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    17941 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18001 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaaccgact
    18061 gaaacttctt gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca
    18121 gcactcgaag cttagtgaat gttttctcca gctcttgcca ctgaacaggt tctggaggcg
    18181 tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa agagatcagc aagcctgcgc
    18241 aggcaaacgc aaaaatgacc gccatgaccg tttttttggc cctccataaa actgcgatta
    18301 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca ggaaaatgag
    18361 cgtcacttcc ctgtttaata ttcagtgatg acatggggta taacctgaaa atgagtccaa
    18421 taggggaaat ctccacggcg ctcactttac cagttggtga taattatccg aagctgaagt
    18481 tt
//
LOCUS       DAZFAH010000082        18474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFAH010000082 DAZFAH010000000
VERSION     DAZFAH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.082"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1554
                     /gene="mltF"
                     /locus_tag="ACHJWB_002643"
     CDS             <1..1554
                     /gene="mltF"
                     /locus_tag="ACHJWB_002643"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4314252.1"
                     /translation="KKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGEL
                     RVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNAD
                     LLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVV
                     NDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAV
                     ALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDT
                     RTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTK
                     NTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLD
                     ARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISL
                     VGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFS
                     RKGSEEKQN"
     gene            complement(1551..2054)
                     /gene="tadA"
                     /locus_tag="ACHJWB_002644"
     CDS             complement(1551..2054)
                     /gene="tadA"
                     /locus_tag="ACHJWB_002644"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4314253.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2112..2747)
                     /gene="yfhb"
                     /locus_tag="ACHJWB_002645"
     CDS             complement(2112..2747)
                     /gene="yfhb"
                     /locus_tag="ACHJWB_002645"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4314254.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2956..3804
                     /gene="yfhH"
                     /locus_tag="ACHJWB_002646"
     CDS             2956..3804
                     /gene="yfhH"
                     /locus_tag="ACHJWB_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4314255.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3860..4120
                     /gene="yfhL"
                     /locus_tag="ACHJWB_002647"
     CDS             3860..4120
                     /gene="yfhL"
                     /locus_tag="ACHJWB_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4314256.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(4314..4394)
                     /gene="shoB"
                     /locus_tag="ACHJWB_002648"
     CDS             complement(4314..4394)
                     /gene="shoB"
                     /locus_tag="ACHJWB_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4314257.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4815..5195)
                     /gene="acpS"
                     /locus_tag="ACHJWB_002649"
     CDS             complement(4815..5195)
                     /gene="acpS"
                     /locus_tag="ACHJWB_002649"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4314258.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(5195..5926)
                     /gene="pdxJ"
                     /locus_tag="ACHJWB_002650"
     CDS             complement(5195..5926)
                     /gene="pdxJ"
                     /locus_tag="ACHJWB_002650"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4314259.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5938..6666)
                     /gene="recO"
                     /locus_tag="ACHJWB_002651"
     CDS             complement(5938..6666)
                     /gene="recO"
                     /locus_tag="ACHJWB_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4314260.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6678..7583)
                     /gene="era"
                     /locus_tag="ACHJWB_002652"
     CDS             complement(6678..7583)
                     /gene="era"
                     /locus_tag="ACHJWB_002652"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4314261.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPVSAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7580..8260)
                     /gene="rnc"
                     /locus_tag="ACHJWB_002653"
     CDS             complement(7580..8260)
                     /gene="rnc"
                     /locus_tag="ACHJWB_002653"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4314262.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8533..9507)
                     /gene="lepB"
                     /locus_tag="ACHJWB_002654"
     CDS             complement(8533..9507)
                     /gene="lepB"
                     /locus_tag="ACHJWB_002654"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4314263.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9523..11322)
                     /gene="lepA"
                     /locus_tag="ACHJWB_002655"
     CDS             complement(9523..11322)
                     /gene="lepA"
                     /locus_tag="ACHJWB_002655"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4314264.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11520..11999)
                     /gene="rseC"
                     /locus_tag="ACHJWB_002656"
     CDS             complement(11520..11999)
                     /gene="rseC"
                     /locus_tag="ACHJWB_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4314265.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11996..12952)
                     /gene="rseB"
                     /locus_tag="ACHJWB_002657"
     CDS             complement(11996..12952)
                     /gene="rseB"
                     /locus_tag="ACHJWB_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4314266.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12952..13602)
                     /gene="rseA"
                     /locus_tag="ACHJWB_002658"
     CDS             complement(12952..13602)
                     /gene="rseA"
                     /locus_tag="ACHJWB_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4314267.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13635..14210)
                     /gene="rpoE"
                     /locus_tag="ACHJWB_002659"
     CDS             complement(13635..14210)
                     /gene="rpoE"
                     /locus_tag="ACHJWB_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4314268.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14207..14362)
                     /gene="rseD"
                     /locus_tag="ACHJWB_002660"
     CDS             complement(14207..14362)
                     /gene="rseD"
                     /locus_tag="ACHJWB_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4314269.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14618..16240
                     /gene="nadB"
                     /locus_tag="ACHJWB_002661"
     CDS             14618..16240
                     /gene="nadB"
                     /locus_tag="ACHJWB_002661"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4314270.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSLLSPGNHYINR"
     gene            complement(16225..16962)
                     /gene="trmN"
                     /locus_tag="ACHJWB_002662"
     CDS             complement(16225..16962)
                     /gene="trmN"
                     /locus_tag="ACHJWB_002662"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4314271.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17094..18428
                     /gene="srmB"
                     /locus_tag="ACHJWB_002663"
     CDS             17094..18428
                     /gene="srmB"
                     /locus_tag="ACHJWB_002663"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4314272.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         4539 a         4887 c         4594 g         4454 t
ORIGIN      
        1 aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct cgcggtcgct
       61 ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg
      121 cggggagagt tgcgtgtgag caccattcat actcccctga cttataacga aatcaacggg
      181 aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct cggtgtaaaa
      241 ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgacctcga taatggtaac
      301 gccgacctgc tggcggcagg actggtctat aacagtgagc gggtaaaaaa ttatcagcct
      361 ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca gtatcgcccg
      421 cgcacactgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca cgtggtggtt
      481 aacgatctcc aaaccctgaa agacacaaaa ttcccggaat taagctggaa ggtagacgac
      541 aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga ttacaccatt
      601 gctgattctg tcgccatcag cctgtttcag cgcgttcatc cggagctcgc cgtagcgctc
      661 gatatcaccg atgaacaacc ggtgacctgg tttagcccgt tagatggcga taataccctt
      721 tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct ggcacgcatt
      781 gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg cacattttta
      841 cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata cgccgaagaa
      901 attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga tgcacaggcc
      961 acttcaccga cgggtgtacg cggcatgatg atgttaacca aaaataccgc gcaaagcctc
     1021 ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag
     1081 gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg agcggatctg gtttgccctc
     1141 gctgcgtaca atatgggcta tgcgcatatg ctggatgccc gcgccctgac ggcaaaaacc
     1201 aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact tagccagaaa
     1261 ccctattaca gcaagctgac ttacggctac gctcgtggac atgaagccta cgcttatgtc
     1321 gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa agagaagcag
     1381 gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc tacggagttg
     1441 ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc
     1501 cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc
     1561 gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg
     1621 agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg gtgattcata
     1681 cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc gtcacgcgca
     1741 ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat tacacatggt
     1801 tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac cagaccaccc
     1861 tgccgcaggg ccatgatttc tgcatgtgcg gtgggatcat ggcgaccaat cgggcggttc
     1921 cagccttcgc cgattacccg attgttatgc actaataccg cgccgaccgg cacttcccgc
     1981 tcatcccagg cacgtttcgc cagcgtcagc gcgtgacgca tccagtattc gtggctaaat
     2041 tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat acacggacgc
     2101 tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg ccaacgatgc
     2161 tgacagaaat aaagcagcgg attgtcctga ttactgtcgc tatagccact gtacagccgc
     2221 agcggagtgc cgattttgcg ctccagttgt gcgacctttt catgtcccag acaacgcatc
     2281 gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt aacccgcggc
     2341 agccagggcg tatcgaaata aaccgcttca accagcggct gcggagagcc ggtaatcaac
     2401 cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac cagcggaaag
     2461 gcggtaacat tgtcgcgaaa ccagcgcacg aaatcggcct gcaacgtctg taaacgtgct
     2521 tcgctgtgac caaaagtgca cccccacaga agcagactca tcggccagcg tgccgcacga
     2581 ccttttatca ataacgcaat ggctataatc ggtaacaacg gcaggacaag taacgcattc
     2641 agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg ctgatgcaat
     2701 gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc atactcctct
     2761 ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag atcatcactt
     2821 ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg gcaattagcg
     2881 gcaatggaat ataaaattca ctcgcgtgtc tctcatattg cctgttgtcg ccactatttc
     2941 atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca ggggcttgcc
     3001 cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc gcgccattta
     3061 agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg
     3121 caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga agcgctggca
     3181 agccagccgg aatcgccctc cgtgcccatt cataaccaaa tccgcggtga tgatccgtta
     3241 cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc aacgctaaac
     3301 gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata
     3361 attctgaccg gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg
     3421 aagattggct tcaatgctgc cgcagtgcgc gatatgcatg cgctactcgc aacagtacag
     3481 gcgtcgtccc ctgacgatct gttattagcc atttcctaca ccggtgtacg acgcgagtta
     3541 aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat taccggcttt
     3601 actccgaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc cgaagaacaa
     3661 gcgacaaaca gtgcttcaat ctctgcttgt cacgctcagg gaatgttaac ggatttgctg
     3721 ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg tcatagtgaa
     3781 gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg
     3841 ttgttttgag agtttcctta tggcgttgtt aatcactaaa aaatgcatca attgtgatat
     3901 gtgtgaaccc gaatgcccga atgaggcgat ttcaatggga gatcatatct acgagattaa
     3961 cagcgataag tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg
     4021 cccgatcccc aatactattg tgaaagatcc ggcgcatgtc gagacagaag aacagttgtg
     4081 ggataaattt gtgctgatgc accacgcgga taaaatttaa tattctactc tggaagtaga
     4141 gtattaatta tattactggg aagccttaac gccattatat ttatttaatt gatgacatta
     4201 gcataatcat tcactaagtt aatttatata gtatctgcca agacacttat ttatagttat
     4261 taaaggcgcg tccgattggt tcaccggacg caccttaagt acgtttcctt gtgttataag
     4321 aacagaagga tcagctgtaa aacagcaatg atgattttga tgacccgttt aatcaggtat
     4381 cggcaatcag tcattcgttt ttccttaaac aaggaatgca gccgttcaga ttagccctta
     4441 catctcccca aaactgaacg tgcgagttat tgagggtgca tgctgcactc cacaccagag
     4501 ctttgacgac accactcgtt tcaatggggg aattctgtgg catggtgtaa agcacagcaa
     4561 aatcttcaat aacgaagcca attttaatgt acttacgaat tggcggtcac gttaatcttt
     4621 ccatcaatat tgctttcttc gtaaaggctc gagtttttat gctaaagatt gcaagttgct
     4681 tgtaaaagat aagtacactg atccataatc gctgttgttg agggtgcatg ctgcacaaaa
     4741 ttaaagttaa aaagtaaaac ccccgttcct taccagttcg ggggttttac tttttaaaga
     4801 gaacggtatt atttttaact ttcaataatt accgtggcac aagcatagtg ccgctcatcg
     4861 gcaagcgtta catgcatatt tacaacgccg agcttttccg ccagttttaa tgcctcaccc
     4921 catagccgta gccgtggttt gccgagctca tcattgaata cttcaaattg attaaacgcc
     4981 agaccattgc ggatcccggt gccaaacgct tttgctgcgg cttctttcac agcaaaacgc
     5041 ttcgccagaa aacgcaccgg ctggtggtgc gttttccaga ttgcccattc gttatcgctt
     5101 aatacgcggc gtgccaggcg ctcaccggat cgggcgatca ctgcttctat gcgagcgatc
     5161 tctacgatat ccgtgcctaa acctaatatt gccattagcc acgcgcttcc agcataagac
     5221 gcttcatttc tgccaccgca tctttcagtc cggtcatcac tgcacgacca ataatggcat
     5281 gaccgatatt cagttcatgc atctcaggga tggcggcaat agctttcacg ttgtgatagg
     5341 tcagaccgtg accagcgtta actttcagac cgagactagc ggcaaaggtc gcggctttgg
     5401 cgatacgcgc cagctcttgc gcctgttcgg cgtcagtttt ggcatcagca tagcaaccgg
     5461 tgtggatctc gataaacggt gcgccaacct ctgccgcagc tttgatctgc tcttcatcgg
     5521 cgtcgataaa gagagaaacc tggatcccgg catctgccag acgtttgcag gcatcgcgca
     5581 ttttgtcacg ctgccctgcg acgtccaggc cgccttcggt tgttacttcc tgacgctttt
     5641 ccggtaccag acagcaaaaa tgtggcttcg tctcaacggc gatcgccagc atctcttcgg
     5701 tcaccgccat ctccagattc atgcgggtat ccagtgtctg acgcaggatg cgcacatcac
     5761 ggtcagtaat gtggcggcga tcttcacgta aatgcacggt aatgccgtcc gctcccgcct
     5821 gctcagcaat aaacgccgcc tgcaccggat ccgggtaagc agtaccgcgt gcgttacgca
     5881 gcgttgcaat atggtcaatg ttgacgccta acagtaattc agccatgaca atcctcatca
     5941 ttcataatgt gttttcaccg ttcgcttagg cataaactgc cggaacagtt ccctgctctt
     6001 taatggttta ccgccaagat acggcttaag cgccatgcgg gtaaagcgtt tcgcggcgcg
     6061 tagtgtgtct gcgtcaggaa attcccgcgc gtttaacgct tttaactgcc ttccggtgaa
     6121 cgttttattg tcgataacga cgcttgcgat aaaccctttt tcttcgcgat aacgatacgt
     6181 catggtgtca tctaccggct cgccgctacc cgcacaatgg gtaaaattga cgccatagcc
     6241 cagatgcccg agcagtgcca gttcaaagcg gcgcagcgcg ggttctggcg taccagtggc
     6301 acctgcaaga gactgaatgc agtgcaagta atcgaaaaag agttcagaga agcgcgtctc
     6361 gtattccagt acgcgggaga gaagttcgtt gatgtacaga ccgctgtaaa gcgtgatacc
     6421 gcttaatggc agcgccagcg aaacagcttc agcactgcgc agcgttttga cttcgccacg
     6481 cccgccgaaa cgtagcaaga gaggggtgaa aggctgtaat gcacctttca gagtagagcg
     6541 tttagagcgt gcgcctttgg caaccagacg cacgcgaccc gattcctccg tgaagacgtc
     6601 cagcatcagg ctggtttcgc tccacgggcg actatgcagg acaaatgcgc gctgccagcc
     6661 ttccatcgga gtgactctta aagatcgtca acgtaaccga gactgcgcag tgcgcgttcg
     6721 tcgtcggccc aaccggattt cacttttacc cacagctcaa ggtgaacagg cgcttcgaac
     6781 atttcctgca tgtctttacg cgcttcaatc ccgatggttt tgatcttggc ccctttgttg
     6841 ccaataacca tcttcttctg cccttcacgc tcaacgagaa tcaaaccgtt gatgtcataa
     6901 ccaccgcgtt cgttagagac aaaacgttcg atctccacgg tcacggagta cggcagttca
     6961 gcgccgagga aacgcatcag tttttcgcgg atgatttcag acgccataaa acgctgtgag
     7021 cgatcggtga tgtaatcttc cgggaagtga tgggtcgctt caggcagatg cttacgcacg
     7081 attgccgcaa tagtgtcaac attcaacccg gtttcggcag agaccggcac gatatcgagg
     7141 aagttcatct ggcttgccag gaactgtaag tgtggcagca gatcggcttt ttcctgcacg
     7201 ttatccactt tgttcaccgc gaggatcacc ggagctttgc catcgcgcag tttgttgagc
     7261 accatttcat cgtccggcgt ccagcgggtg ccttctacga caaaaatgac cagctcaacg
     7321 tcgccaatgg agctgctcgc cgctttgttc atcaggcggt taatggcgcg tttttcttcc
     7381 atatgcaggc ccggggtatc gacgtagatc gcctgatacg cgccttcagt atggatcccc
     7441 acaatgcggt gacgagttgt ttgcgccttg cgggaggtga tggagatctt ctgccccagc
     7501 agtttattca acaatgtgga tttgccaaca ttcggacgtc cgacgatagc aataaatccg
     7561 cagtaacttt tatcgatgct cattccagct ccagtttttt caacgcctgt tcggcggcag
     7621 cctgctcagc cttacgacgg cttgaacctg tgcctaccac cggttcactc aggccgctga
     7681 cctggcagtg gatagtaaat tcctgatcgt gcgcttcgcc acgtacctgg actaccagat
     7741 aagtcggcag cggcagatgg cgaccctgca aatattcttg caagcgcgtt ttcggatctt
     7801 tttgtttatc gcctgggcta atttcgtcca gacgggtttg ataccagtta aggattaatt
     7861 tctcgacggt ttgaatatca ctgtcgagga atacgccacc aattaatgct tcgacggtgt
     7921 cggcgagaat tgactcacga cgaaatccac cgcttttaag ttcacctggc cctaaacgta
     7981 agcactcgcc taactcaaat tcgcgcgcca gttctgccag cgtattgcca cggaccagcg
     8041 tggcgcgcat ccggctcata tcgccttcat ccacacgagg gaaacggtga taaagcgcat
     8101 tggcgataac gtagctcaga atagagtcgc ctaaaaattc taaacgctcg ttatgtttgc
     8161 tgctggcact acgatgagtt aatgcctgct gcaacagttc ctgatgatta aaagtgtagc
     8221 ccagcttccg ttgaagccga ttaattacga tggggttcat gcgataccaa taaataaatg
     8281 cgtcaacaat tcagcacacg aaacagacct gatatacatg gctctgctaa ctgcttcgct
     8341 gcagtttcag tttaccggta tatggggacc aacgctgttt cgtgtgccgt ggcaacctgg
     8401 aggtgccaac cttaaacttc gggggaatat tctatacaca acgacggggg atgtcgttag
     8461 ccacgggaga tttatctcat aaataattca cgttgtcgcc ataacggcga caacgtgaac
     8521 gaagatgggc tattaatgga tgccgccaat gcgacttaag cgcacacctg tcggccattc
     8581 gccttcttgc ttatcgaaac tcatccagat agccgttgcc cgaccgacca gattcgcttc
     8641 aggcacaaaa ccccagtaac ggctgtccgc gctgttgtcg cggttgtcgc ccatcatgaa
     8701 gtattgtccc ggcggaacaa tccaggttgc cagttgttgc cctggctgct ggtaatacat
     8761 ccccacctgg tcctgcgcaa tcggcactgt cagaattcgg tgcgtcacat cacccaatgt
     8821 ctctttacgc tcggaaagac gaattccatt ttctttggtt tcgtttttcg gcacttcaaa
     8881 gaatccgctg gtcgcttccc caccattacg gcgtgagaag gtctgaacga aatcgctcgg
     8941 ttccacgttg gagtaggtga ccggcagcgc gttttcacat gcctggccgg aactgcatcc
     9001 cggttgaatc gtcagctctt tcgagaccgg atcataagta actttatcgc ccggtaaacc
     9061 caccgcgcgc ttgatgtaat caagctttgg atcttccgga tatttaaaga ccacgatatc
     9121 tccgcgtttc ggatgaccgg tttcgatcag cgttttctgg tagataggat ctttaatgcc
     9181 ataagcaaac ttttctacca gaataaaatc accgattaac agagtcggca tcatcgaacc
     9241 tgacgggatc tggaacggtt cataaataaa cgaacgcaca atcaatacga tagccagtac
     9301 cggaaaaaca gaagcaccgg tttccagcca gccaggcttc ggcgcaacct ttttcaacgt
     9361 tgctttatcc agtgagtcac cggcagccgc ctgcgccgct gcctgacgtt cccgccgttt
     9421 aggtgcgaaa aagaatttat ccacgcacca taaaatgccc gtcaccagtg tggcaatcac
     9481 cagaatcagg gcaaacatat tcgccatgcc aactcctaag ggttatttgt tgtctttgcc
     9541 gacgtgcaga atggcgagga acgcttcctg cggcagctcg acgttaccga tctgcttcat
     9601 gcgtttctta ccttctttct gcttctgcag cagctttttc ttacggctga tatcgccgcc
     9661 ataacattta gccagtacgt ttttacgcag ctgtttcacg gtggatcgcg caataatgtg
     9721 cgtaccaatc gctgcctgaa tggcgatatc aaactgctgg cgtgggatca gatctttcat
     9781 cttctccacc aactcgcgac cgcggttttg cgaattatca cggtgggtga tcagcgccaa
     9841 cgcatcaaca cgttcaccgt tgattaatac gtctacacgt accatgtcgg acgcctggaa
     9901 gcgtttgaag ttgtaatcca gagacgcata accacgcgag gtagatttca ggcgatcgaa
     9961 gaagtcgagc accacttccg ccatcgggat ctcgtacgtc agcgccacct gattaccgtg
    10021 gtaaaccata ttggtctgca cgccgcgttt ttctacgcac agcgtaataa cgttgccgag
    10081 atatgcctgc ggcagcagca tgtgacattc ggcaatcggc tcgcgcagtt cgtagatgtt
    10141 atttaccgct ggcagcttgg atggactgtc gacgtaaata acttctcttg acgtggtttc
    10201 aacttcatac actacggtcg gcgcagtggt gatcagatcc agatcgtatt cacgttccag
    10261 acgttcctgg atgatctcca tgtgcagcag gccgaggaag ccgcagcgga aaccaaagcc
    10321 cagcgcgctg gagctttccg gctcatagaa cagtgaagca tcgttcaggc tgagtttacc
    10381 cagcgcgtca cggaaggctt catagtcgtc ggaacttacc gggaacagac cggcgtatac
    10441 ctgcggtttg actttcttaa agccaggcag cgccttttct gccggattac gcgccagcgt
    10501 taaggtatcg ccgactggag cgccgtggat atctttaatc gcacatacca gccagcctac
    10561 ttcgccacat ttcagttcag tgcggtcaac ctgtttcggc gtgaagatgc ccagacggtc
    10621 ggcgttatag gtctgcccgg tactcatgac tttcactttg tcgcccttac gcagggtgcc
    10681 gtttttaata cggataagtg aaacaacgcc caggtagttg tcgaaccatg agtcgataat
    10741 tagtgcctgc aacgggcctt ccggatcgcc ttccggcggc ggaatgtcgc gcaccagacg
    10801 ttcgagaacg tcctgcacgc caacgccggt tttcgctgaa cagcgcaccg cgtcagtggc
    10861 gtcgatgccg acgatatctt caatttcttc cgccacgcgt tcaggatcgg ctgccggcag
    10921 gtcaatcttg ttcagtactg gcaccacttc gagatccatt tccatggcgg tgtagcagtt
    10981 tgccagggtt tgcgcttcca cgccctgccc ggcgtcgacc accagcaatg caccttcaca
    11041 ggcagccagc gaacgggaaa cttcatagga gaagtctacg tggcccgggg tgtcgataaa
    11101 gttaagctga taggtttcgc cgtcagacgc tttgtagtcc agcgtcacgc tttgcgcttt
    11161 gatggtaatg ccacgctcac gctcaagatc catggaatcg agaacctgcg cctccatttc
    11221 acggtcagac aggccaccgc agatctggat aatacggtca gacagcgtcg atttaccgtg
    11281 gtcaatgtga gctatgatcg aaaagttacg tatattcttc ataaagtatg attattgtgc
    11341 cttaatgccc ggttaaccag gcttttagaa gtcgctgttc tgagcttaac gtctgtatta
    11401 atagaaacgc cgcattctac actacaacat tgaggcgagg aaatgttcat accgtatgga
    11461 ttgtggtatc tggaaacgtc ctcgcatttg ttatgcaaaa tgcaacaaag ccagtgaaat
    11521 cactggctcg cgtcttccga agatgtttca aatcgcacaa ggccaggcgg caatgccacg
    11581 cttaagatga tcggttgcca ttctgcccgg gcagcaaact tacgcgagta gccgcgcgca
    11641 atcaggaacc cgccaatacc accgaggatc gcaccacata atgctgcgac gtcagaagca
    11701 aagagtagct gaaatagcga agcgataagg aataatccca ccagcggcga catataaacc
    11761 agtaatgcgg agctaagcag gctgccttcg gcgatcccca attccacttt ttgccccggc
    11821 actaacggct catcacaggg tacgacaatg gtatgcgtgg tttgcgggcc aagtttattt
    11881 aacacgcggc taccgcaacc ggcgcgtgaa gcgcagctgc tgcatgaagc tttaacatca
    11941 caactgacca gcgcctgccc gttttgccag gagacgacgg tagcccactc tttgatcatt
    12001 gcgctgcccc gaacttaata ttctcggcaa tgcgtttcgc cgtttgcggc ggcagttcac
    12061 cgacaatggt gatttcggcg ttatcacgta cgcttgtact gacggttctg cgtccggtgc
    12121 gcaacatctg atcggtgctc gatggcgtag cgcggttaac gtttaccgag aagctgaata
    12181 atccgtcgga atagagacgt gattcgatag gcatgttgtc catcgtcggt agcggacgtc
    12241 gactactgga aacttcgcta aaaccctgtg gcaaccaggt tggcgtccag ctgaatttag
    12301 ctttttcacc tacaggaaca gaaagcaacg gcggcaaatt tgccttcgcc agcgtctgca
    12361 tactgctgct gatatcctga ttgacgttaa aagcaatcac gcgaaattgt tccagcgttt
    12421 caccatcgcg atcaaggaga tcaacccgca tcggtaattt cgattcggtg tccatccaca
    12481 cgatgtagct gtagcgtgta ccatctcggg caaccacgcg aatgacttcg caaagacgat
    12541 cagcaatacg cgtgcgcccg acggagataa agtcgtagta aggagaaagg cgtttgaaat
    12601 cggtatagat aagcgatggc agagaatcaa caatgtaatc gccattaagc gtgaacggtt
    12661 caagtcccgg ttcaaaatag ctgatttcat tgccgcgctg taccacttcc cggcgcgggc
    12721 catccatttg caacaattgt gcaagaggac ggttatcgag gcgtgcatgt cgataacgca
    12781 gagactcaac accctgttta ttgatgctga tgaatgacag ctcgtaattc agtgactgac
    12841 tggccaggtt catctgctgt aataacgccc cggacgcggg agtggccgag gcgttagcag
    12901 agaataacag gctacctgtc actaatgaca tggcaaacca aagttgcttc attactgcga
    12961 ttgcgttcct aaagtttgaa ttcctggcac ctgtacagcg gcttgctggg tttgcgcctg
    13021 ctcaaactga agctgttcag agtggagtcg gcgttgcagt tcgtaatcct gcaacattgc
    13081 attaatgcga cgacgctgct cctgtacctg ctgctgttga ccattgtttg cggtcgcttc
    13141 agaaggtact cccaggctta ccgggctggc tttacccatc atcggcagtg tattaaatac
    13201 cggcgtttcg ggctgctggg acgtttcaga ttgtccatta tagtgctgga cgccaacgat
    13261 aactgcaagc gatacgcatg ctgctacgcc catttgggta agctgtgccg cccacggacg
    13321 tactttctgc cagaatggca ttttctgcca ttgatgcggc gcaggctggg cttccgggat
    13381 caatgtcgcc ggttgacgta ctggctcttc ttcaatggcg gccatcacgc gtgaagagat
    13441 atcgaaatgg agcacctcgg gagtatcacc ccgcattgaa tcacggatta agtgatagct
    13501 ttcccaggtt ttttgcattt ctgggttatg agccagttcg ttaagcagct cactatccag
    13561 cgtttcgcca tccattaaag cggaaagttg ttctttctgc atgcctaata cccttatcca
    13621 gtatcccgct atcgtcaacg cctgataagc ggttgaactt tgttatcaat agcttccctc
    13681 gctcggaaga tacgtgaacg caccgtacct accggacaat ccatgatagc ggctatctct
    13741 tcatagctca ggccatccag ctcccgcaag gttattgcca tgcgcaaatc ttccgggagg
    13801 gactcaatag ttcggaaaac tatctgtctc agttcttctg acaacattaa gttctcaggg
    13861 ttcgaaattt ctttcaacgc gccgccactt tcgaagtttt cagcttcaat ggcatccaca
    13921 tcactggaag gtggacgacg cccctgagca accaggtaat ttttcgctgt atttacagca
    13981 atccgataca gccatgtata aaaagcgcta tctccccgga acgaatccag cgcacgatag
    14041 gctttaataa aagcttcttg taccacatcg ggaacatcac ccgacggcac atagcgggaa
    14101 accagactcg ccactttatg ctgatagcgc actaccagta agttaaaggc tttctgatct
    14161 cccttctgga cccgttcaac caggacctgg tccgttaact gctcgctcat ccgaggtaaa
    14221 gtctccccaa accaaatttc cacgcgctat cgaaacgcca ctccattagc tgcaatttga
    14281 gcaagcaaag ggttagagtg tctcgttttt gtaaagttcc gtaacgcatc tgtttttgtt
    14341 tgtcatgctg tagacggatc attatctatc attataagtc tacagaatct gaacatcgca
    14401 ttatctgtgt agaaatgccc atttaactgc ctgaagagta acccaacggc ctttttattt
    14461 caccacctaa tcctccacca gccagtaact tctctttttc tcgccgccct gcatcagcgt
    14521 gtttagcaac tgtaacaaat attaaaatag caggtgttta ttcgcacaac atgatgctat
    14581 gctgaccaaa ccgtgtttag taaattaaac aaagaaaatg aatactctcc ctgaacattc
    14641 atgtgacgtg ttgattattg gtagcggcgc agccggactt tcactggcgc tacgcctggc
    14701 tgaccagcat caggtcatcg ttctaagtaa aggcccggta acggaaggtt caacatttta
    14761 tgcccagggc ggtattgccg ccgtgtttga tgaaactgac agcattgact cgcatgtgga
    14821 agacacattg attgccgggg ctggtatttg cgatcgccat gcagttgaat ttgtcgccag
    14881 caatgcacga tcctgtgtgc aatggttaat tgaccagggg gttttgtttg atacccacgt
    14941 tcaaccgaat ggcgaagaaa gctaccatct gacccgtgaa ggtggacata gtcaccgtcg
    15001 tattcttcat gccgccgacg ccaccggtag agaagtacaa tccacgctgg tgagcaaggc
    15061 gcagaaccat ccgaatattc gtgtgctgga gcgcagcaac gcggttgatc tgattgtctc
    15121 tgacaaaatt ggcctgccgg gcacgcgacg ggttgttggc gcgtgggtat ggaaccgaaa
    15181 taaagaaacg gtggaaacct gccacgcaaa agcagtggta ctggcaaccg gcggtgcgtc
    15241 gaaggtttat cagtacacca ccaatccgga tatttcttct ggcgatggca ttgctatggc
    15301 gtggcgcgcg ggctgtcggg ttgccaatct cgaatttaat cagttccacc ctaccgcgct
    15361 gtatcaccca caggcacgca atttcctgtt aacggaagca ctgcgcggcg aaggcgctta
    15421 tctcaagcgc ccggatggca cgcgttttat gcccgatttt gatgagcgcg gcgaactggc
    15481 cccgcgcgat attgtcgccc gcgccattga ccatgaaatg aaacgcctcg gcgcagattg
    15541 tatgttcctc gacatcagcc ataagcccgc cgattttatt cgccagcatt tcccgatgat
    15601 ttatgaaaaa ttgctcgggc tggggattga tctcacacaa gaaccggtac cgattgtgcc
    15661 tgctgcacat tatacctgcg gtggtgtaat ggttgatgat catgggcgta cggacgtcga
    15721 gggcttgtat gccattggcg aggtgagtta taccggctta cacggcgcta accgcatggc
    15781 ctcgaattca ttgctggagt gtctggtcta tggctggtcg gcggcagaag atatcaccag
    15841 acgtatgcct gatgcccacg gcgtcagtac gttaccgccg tgggatgaaa gccgcgttga
    15901 gaaccctgac gaacgggtag taattcagca taactggcac gagctacggc tgtttatgtg
    15961 ggattacgtt ggcattgtgc gcacaacgaa gcgcctggaa cgcgccctgc ggcggataac
    16021 catgctccaa caagaaatag acgaatatta cgcccatttc cgcgtctcaa ataatttgct
    16081 ggagctgcgt aatctggtac aggttgccga gttgattgtt cgctgcgcga tgatgcgcaa
    16141 agaaagccgg gggctgcatt ttacgctgga ttacccggaa ctgctcacgc attccggtcc
    16201 atcgctcctt tcccccggca atcattacat aaacagataa aaagcctggg tcagcgccgt
    16261 atacgcttcg gaatagttct ggtctggccc acgaatgact aagcgatcgc taaagcattc
    16321 tcccgcctgc ggggagaatg ccagcagcac ccgatgcggc agtcgcgctt cgttttccgc
    16381 cacatccgtc cgcaaacgta aatgccagcc catgcttaat gccagttcag taaaaccatt
    16441 accaatctgc tctggcagca ccacgcagaa aaatccctct tcggtaatgc actccgccgc
    16501 acaggtcagc agcgatgggt gatcaagcgt agtggtatag cgagcctgtt cccgttgagg
    16561 tgtcgcgcac tctactccct gctgatagta aggtgggtta ctgatgatta aatcgaagcg
    16621 tactgtctgc tgtgtgatcc actgctgaat atccgccgta tggacgttaa tccgctctgc
    16681 ccacggggac tggttgatat tttcctgcgc ctgcgcggca gcttcacttt ccagctcaac
    16741 ggcatcaatt atcacgctgt cactggttcg ctgcgccagc atcagtgcca gcaacccgct
    16801 acccgcgccg atatcaaggc aacgtttcac cccagccacc ggtgcccatg cgcccagcaa
    16861 aatgccatcc gttcccactt tcatcgcaca gcgatcgtga gcaacaaaaa actgtttaaa
    16921 agtaaatcca ttacgacgaa gcacggatgt agactgtgac atgaaaataa aaccttgcag
    16981 gaaaaacggc gatagcaccg ggtgagaaca atacccgaga agcgatatcc atacaaacag
    17041 atgaagattg cagccgtaac gtctataatc agcgccccac acagaggtag aacatgactg
    17101 taacgacttt ttccgaactt gaactcgacg aaagcctgct ggaagccctc caggataaag
    17161 gtttcactcg cccgaccgcc attcaggctg ccgccattcc gcctgcgctc gatggccgtg
    17221 atgtactcgg ttctgcgccg acaggcaccg gtaaaacggc ggcgtatctg ctgccagcgt
    17281 tgcagcacct gctcgatttc ccgcgtaaga aatccggtcc gccgcgtatt ttgatcctca
    17341 ccccaactcg cgagctggcg atgcaggtgg ccgatcatgc ccgcgaactg gcgaaacata
    17401 cgcatctgga tatcgccacc atcaccggcg gcgtagccta tatgaaccac gcggaagtgt
    17461 tcagcgaaaa ccaggacatc gtggtcgcca cgaccgggcg tctgctgcaa tacataaaag
    17521 aagagaactt cgattgccgc gcggttgaaa cgctgatcct cgacgaagca gaccgtatgc
    17581 tggatatggg cttcgctcag gatatcgaac atattgctgg cgaaacgcgc tggcgtaaac
    17641 agaccctgct cttttcggca acgctggaag gcgatgcgat tcaggacttt gccgagcgtc
    17701 tgctggaaga cccggtggag gtttctgcca atccgtccac ccgtgagcgc aaaaaaattc
    17761 atcagtggta ttaccgcgcc gatgatcttg agcataaaac cgcgttgctg gtgcatctgt
    17821 taaaacagcc ggaagcgacc cgctcaattg tgtttgtgcg taagcgtgaa cgtgtgcatg
    17881 agctggcaaa ctggctgcgc gaagcgggca tcaacaactg ctatctcgaa ggtgagatgg
    17941 tacaaggcaa gcgtaacgaa gcgatcaagc gtttgaccga aggtcgcgta aacgtactgg
    18001 ttgcaaccga tgttgccgcg cgcggtatcg acattcctga cgtcagccac gtctttaact
    18061 tcgatatgcc gcgcagtggt gatacttatt tgcaccgtat cggacgtacc gcgcgcgccg
    18121 gtcgtaaggg aacagcgatt tcgctggttg aagctcatga ccatctgctg ctgggtaaag
    18181 taggccgcta tattgaagag ccaattaaag ctcgcgttat tgatgagtta cgcccgaaaa
    18241 cgcgtgcgcc aagcgaaaag cagaccggca agccatcgaa gaaagtactg gctaaacgtg
    18301 ccgagaagaa aaaagctaaa gagaaagaga agccgcgggt gaaaaaacgc catcgcgaca
    18361 ccaaaaatat tggtaagcgc cgtaaaccaa gcggaacggg cgtgccaccg caaacgacag
    18421 aagagtaatc tcaatgccgg gtttaagcct ggtattaaaa gtgctggcaa acgc
//
LOCUS       DAZFAH010000083        18374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFAH010000083 DAZFAH010000000
VERSION     DAZFAH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.083"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..190
                     /locus_tag="ACHJWB_002664"
     CDS             <1..190
                     /locus_tag="ACHJWB_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO4314273.1"
                     /translation="LLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT
                     SLVDNALDLAEITEEYSH"
     gene            209..907
                     /locus_tag="ACHJWB_002665"
     CDS             209..907
                     /locus_tag="ACHJWB_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4314274.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            958..1617
                     /locus_tag="ACHJWB_002666"
     CDS             958..1617
                     /locus_tag="ACHJWB_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4314275.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1635..2033
                     /locus_tag="ACHJWB_002667"
     CDS             1635..2033
                     /locus_tag="ACHJWB_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4314276.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2043..2681
                     /locus_tag="ACHJWB_002668"
     CDS             2043..2681
                     /locus_tag="ACHJWB_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4314277.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2684..3847
                     /locus_tag="ACHJWB_002669"
     CDS             2684..3847
                     /locus_tag="ACHJWB_002669"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4314278.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLLRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            3931..5556
                     /locus_tag="ACHJWB_002670"
     CDS             3931..5556
                     /locus_tag="ACHJWB_002670"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4314279.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5673..5948)
                     /gene="yjfN"
                     /locus_tag="ACHJWB_002671"
     CDS             complement(5673..5948)
                     /gene="yjfN"
                     /locus_tag="ACHJWB_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4314280.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6097..6426)
                     /gene="bsmA"
                     /locus_tag="ACHJWB_002672"
     CDS             complement(6097..6426)
                     /gene="bsmA"
                     /locus_tag="ACHJWB_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4314281.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6608..7357
                     /gene="yjfP"
                     /locus_tag="ACHJWB_002673"
     CDS             6608..7357
                     /gene="yjfP"
                     /locus_tag="ACHJWB_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4314282.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQFGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7354..8109)
                     /gene="ulaR"
                     /locus_tag="ACHJWB_002674"
     CDS             complement(7354..8109)
                     /gene="ulaR"
                     /locus_tag="ACHJWB_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4314283.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8217..9281)
                     /gene="ulaG"
                     /locus_tag="ACHJWB_002675"
     CDS             complement(8217..9281)
                     /gene="ulaG"
                     /locus_tag="ACHJWB_002675"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO4314284.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9635..11032
                     /gene="ulaA"
                     /locus_tag="ACHJWB_002676"
     CDS             9635..11032
                     /gene="ulaA"
                     /locus_tag="ACHJWB_002676"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4314285.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11048..11353
                     /gene="ulaB"
                     /locus_tag="ACHJWB_002677"
     CDS             11048..11353
                     /gene="ulaB"
                     /locus_tag="ACHJWB_002677"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4314286.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11363..11827
                     /gene="ulaC"
                     /locus_tag="ACHJWB_002678"
     CDS             11363..11827
                     /gene="ulaC"
                     /locus_tag="ACHJWB_002678"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4314287.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11841..12491
                     /gene="ulaD"
                     /locus_tag="ACHJWB_002679"
     CDS             11841..12491
                     /gene="ulaD"
                     /locus_tag="ACHJWB_002679"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4314288.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12501..13355
                     /gene="ulaE"
                     /locus_tag="ACHJWB_002680"
     CDS             12501..13355
                     /gene="ulaE"
                     /locus_tag="ACHJWB_002680"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4314289.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVNFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            13355..14041
                     /gene="ulaF"
                     /locus_tag="ACHJWB_002681"
     CDS             13355..14041
                     /gene="ulaF"
                     /locus_tag="ACHJWB_002681"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4314290.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVLEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            14138..14689
                     /locus_tag="ACHJWB_002682"
     CDS             14138..14689
                     /locus_tag="ACHJWB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4314291.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(14764..15039)
                     /gene="yjfY"
                     /locus_tag="ACHJWB_002683"
     CDS             complement(14764..15039)
                     /gene="yjfY"
                     /locus_tag="ACHJWB_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4314292.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDNVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            15366..15761
                     /gene="rpsF"
                     /locus_tag="ACHJWB_002684"
     CDS             15366..15761
                     /gene="rpsF"
                     /locus_tag="ACHJWB_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4314293.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15768..16082
                     /gene="priB"
                     /locus_tag="ACHJWB_002685"
     CDS             15768..16082
                     /gene="priB"
                     /locus_tag="ACHJWB_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4314294.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            16087..16314
                     /gene="rpsR"
                     /locus_tag="ACHJWB_002686"
     CDS             16087..16314
                     /gene="rpsR"
                     /locus_tag="ACHJWB_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4314295.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            16356..16805
                     /gene="rplI"
                     /locus_tag="ACHJWB_002687"
     CDS             16356..16805
                     /gene="rplI"
                     /locus_tag="ACHJWB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4314296.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16917..17888)
                     /locus_tag="ACHJWB_002688"
     CDS             complement(16917..17888)
                     /locus_tag="ACHJWB_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO4314297.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKEEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(17941..>18374)
                     /locus_tag="ACHJWB_002689"
     CDS             complement(17941..>18374)
                     /locus_tag="ACHJWB_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFO4314298.1"
                     /translation="SSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
BASE COUNT         4425 a         4422 c         4847 g         4680 t
ORIGIN      
        1 cttattaaga aatcagaaga tgatgccgtt gtcatcggta ggaatctcca gcgtgcaaca
       61 ggaagagtat tacattgttt tcggggcgtt atcgcttaaa tcttctcttg aagatatcct
      121 gcttgagata acctcgctgg tagataacgc attggatctg gctgaaatta cagaagaata
      181 ttcacactaa ttacaaggac gggtatttat gggaatttta aaaagtttat ttacgctggg
      241 gaaatcgttt atctcccagg cggaagaatc cattgaagaa acccagggcg tgcgcatgct
      301 ggaacagcat attcgtgacg ctaaagctga actcgataaa gccggaaaat ctcgcgttga
      361 tctgctggcg cgggtgaaat taagtcacga taagctgaaa gatttacgtg agcgcaaagc
      421 cagtctggaa gcccgtgcgc tggaagcgtt aagcaagaac gttaatccgt cgttgattaa
      481 cgaagttgct gaagaaatcg cgcgccttga gaatctcatt accgctgaag agcaagtgtt
      541 gtcgaatctg gaagtctccc gtgatggcgt ggaaaaagca gttacagcga cagcgcagcg
      601 tatcgctcag tttgagcagc aaatggaagt cgttaaagcc actgaagcca tgcagcgtgc
      661 acaacaggct gtaacaacct ctaccgttgg cgcatcttcc agcgtttcga cagcggcaga
      721 atccttaaaa cgcctgcaaa cgcgtcaggc cgaacgtcag gctcgcctgg atgctgctgc
      781 acagttggaa aaagtcgcag acggtcgcga tcttgatgaa aagctggcgg aagccggaat
      841 tggcggtagc aataaaagta gcgcccagga tgtattagca agactgcaac gccaacaggg
      901 cgagtaattt ttttgccagc ctcgctaaaa ggctggcaac tattttaagg ataaaatatg
      961 tctggttttt tccagcgtct gtttggcaag gataataagc ctgctatcgc tcgcggtccg
     1021 ctgggacttc atctcaatag tggctttacg ctcgacacgt tagcgtttcg tttgctggaa
     1081 gatgaattgc tgattgcgct gccgggcgag gaatttacgg tagccgctgt cagccacatc
     1141 gatctgggcg gcggtagtca gattttccgc tactacactt caggcgatga atttctgcaa
     1201 atcaatacca ccggcggcga agatattgat gacattgatg atatcaagct ctttgtctat
     1261 gaagagagct acggtatcag taaagagagt cactggcgcg aggcgatcaa cgccaaagtg
     1321 atgggggcaa tgatcttaaa ctggcaggaa aaacgctggc agcgattttt taacagcgaa
     1381 gaaccgggaa atatcgaacc cgtctacatg ctggaaaaag tagaaaatca aaaccatgcc
     1441 aaatgggagg tccataattt taccatgggc taccagcggc aagtaaccga agatacttac
     1501 gaatatttgc tgttaaatgg tgaggaatct tttaacgatc tcggcgagcc agagtggtta
     1561 ttttcgcgcg cgttgggtgt cgatatcccg ctgacatcac ttcatattat tggttaatta
     1621 caaggacgtt tacaatgcat atactggatt cacttcttgc ctttagcgcc tattttttta
     1681 ttggcgtggc tatggtgatt atttttcttt ttatctattc taaaattaca ccgcacaacg
     1741 aatggcagtt aatcaaaaac aataataccg cggcttcact ggcgttcagt ggtacattgt
     1801 taggttacgt tatcccatta tccagtgcgg caatcaatgc ggtgagtatt ccagactatt
     1861 tcgcctgggg cgggattgca ctggtgattc agttactcgt ttttgctggt gtcagacttt
     1921 atatgcccgc attaagcgaa aaaattatta atcacaatac cgcagcagga atgtttatgg
     1981 gaaccgccgc gctggctggc ggtattttta acgcagcttg tatgacatgg taatggacga
     2041 tcatggccag aaaacgcaaa tcaagaaata acagtaaaat tggtcacgga gcgatcagtc
     2101 gcattggtag accgaataat ccttttgaac cgcgtcgcaa tcgctacgca caaaaatact
     2161 taacgttggc gctaatgggc ggtgccgctt tttttgtatt gaaaggttgt ggcgatagtg
     2221 gcgatgtcga taacgacggc gacggaacgt tttattcgac agtgcaggat tgtattgatg
     2281 acggtaataa tgccgacatt tgcgcgcgtg gctggaacaa cgccaaagcg gcattttatg
     2341 ccgatgttcc gaagaatatg actcagcaga attgtcagtc taagtacgaa aattgctact
     2401 acgacaatgt tgaacagagt tggatcccgg tcgtttctgg atttttgtta agccgggtaa
     2461 ttcgtaaaga tcgcgatgag ccgttcgtct ataacagcgg cggatcctca tttgcttccc
     2521 gccccgtctg gcgcaacact tccggtgatt actcctggcg ctccggttct ggcaaaaaag
     2581 agtcttactc ttcaggcggc tttaccacca aaaaagcgtc aaccgtttcg cgcggcggct
     2641 atggtcgttc ttccagcgcc cgtgggcatt ggggaggcta atcatgctga gacacaacgt
     2701 tcctgtgcga cgggatctgg accagatcgc tgctaacaac ggtttcgact ttcatatcat
     2761 cgacaatgaa atctattggg acgagagtcg ggcttaccgt tttactctgc gccagattga
     2821 agagcagatc gaaaaaccga ctgcggaact gcatcagatg tgccttgagg tggtggatcg
     2881 cgcggttaaa gacgaagaga tcctgacgca actggcgatc ccgccgttgt actgggatgt
     2941 gatcgccgaa agctggcgcg cacgcgatcc ttcgctgtat ggccggatgg attttgcctg
     3001 gtgtggcaat gcgccggtga agctgctgga gtacaacgcc gatacgccga cctcgttgta
     3061 tgagtcggct tatttccagt ggttgtggct ggaggatgcc cggcgcagcg gcattattcc
     3121 gcgtgatgcc gatcagtaca atgccattca ggaacgcctg atttcacgct ttagcgagct
     3181 ttatagccgg gagccgtttt atttctgctg ctgtcaggac accgatgaag acaggactac
     3241 cgtgctgtac ttgcaggact gcgcccagca ggcagggcag gagtcgcggt ttatctacat
     3301 tgaagatctc ggtttgggcg tcggcggtgt actgaccgat cttgatgata atgtcatcca
     3361 gcgtgcattt aagctgtatc cgctggagtg gatgatgcgt gacgataacg gtccgctgct
     3421 gcgcaagcgt cgcgagcaat gggtggagcc gttatggaaa agtatcttga gtaataaagg
     3481 gctaatgccg ctgctttggc gcttcttccc tggtcatcct aaccttcttg catcctggtt
     3541 tgagggtgaa aaatcgcaga ttgccgctgg cgaaagctat gtgcgtaaac cgatctactc
     3601 gcgcgaaggc ggaaacgtca ccattttcga cggtcagaat aacgtcgttg accacgctga
     3661 tggtgattac gccgatgaac cgatgatcta ccaggcgttt caacctctgc tgcgatttgg
     3721 cgatagctac acactcatcg gtagctggat tgtcgatgat gaagcgtgcg gaatggggat
     3781 ccgtgaagat aacacactga tcaccaaaga cacctcacgt ttcgttccgc attacattgc
     3841 tggataagaa tgttttagca atctctttct gtcatgaatc catggcagtg accatactaa
     3901 tggtgactgc cattgatgga gggagacaca gtgcactggc aaactcacac cgtttttaat
     3961 caacctatac cattaaataa cagcaactta tacctgtctg atggcgcgct ctgcgaagcg
     4021 gtaacgcgtg aaggtgctgg ctgggatagc gattttctag ccagtattgg tcagcagtta
     4081 ggaacggctg aatcccttga actggggcgg ctggcgaatg tgaatccgcc tgaattattg
     4141 cgctacgatg cgcaaggacg ccgtctggac gatgtgcgtt ttcaccccgc ctggcacctg
     4201 ctgatgcagg cgctatgtac caatcgggtg cacaatcttg cctgggaaga agacgctcgc
     4261 tccggcgcat ttgtggcgcg cgcggcgcgt tttatgttgc acgcacaggt tgaggcaggg
     4321 tcgttatgtc cgataaccat gacctttgcc gccacgccat tgctgttaca gatgttaccc
     4381 gcgccgtttc aggactggac cacgccgctg ctgagcgatc gctacgattc tcacttattg
     4441 ccaggtgggc aaaaacgcgg tttgttgatt ggcatgggaa tgacggaaaa gcagggcggt
     4501 tccgatgtca tgagcaacac cacccgcgca gagcgcctgg aagatggctc ttatcggctg
     4561 gtggggcata aatggttttt ctcggtgccg caaagcgatg cgcatctggt gctggcgcag
     4621 actacgggcg gtttgtcctg cttttttgtg ccgcgctttt tgcctgacgg gcaacgcaac
     4681 gcgattcgcc tcgagcggct gaaagataag ctgggtaatc gctctaacgc cagctgcgaa
     4741 gtggagtttc aggatgccat tggctggttg ttggggcagg aaggggaagg aattcgtctg
     4801 atcctgaaaa tgggtgggat gacgcgtttt gattgcgccc tgggtagcca tgccatgatg
     4861 cgccgtgcat tttcgctggc gatttatcat gcacatcaac gccatgtttt tggtaatcca
     4921 ttgatccaac agccccttat gcgtcatgtc ttaagtcgca tggcacttca gcttgaaggg
     4981 caaacggcgt tgctgtttcg tcttgcgcga gcgtgggacc ggcgtgccga tgccaaagaa
     5041 gccctgtggg cgcgtttatt tacgcctgcg gcaaaatttg tgatctgcaa aagaggtatg
     5101 ccgtttgtgg ccgaagcgat ggaggtgctg ggcggcattg gttattgcga ggagagcgag
     5161 ctgccgcggc tttaccggga gatgccggta aacagtattt gggaaggttc cggcaatatt
     5221 atgtgcctgg atgtgctgcg cgttctcaat aagcaagcgg gcgtatacga cttattgtcg
     5281 gaagcgtttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt tcgtcgttta
     5341 cagcagcagc tgcgcaagcc agctgaagaa ctggggcgag agattactca tcagctattc
     5401 ctgctgggct gcggtgcgca aatgttgaaa tatgcctctc cgccaatggc gcaggcgtgg
     5461 tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat ccagaatgat
     5521 ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga tgcgacgctg
     5581 gtttcgatta actcaatgaa atatgcgaaa attgtaggcc ggacaaggcg ctcgcgccgc
     5641 atccggcatt gttcatttgc tgtatataac actcatgcat acagtatcgc ctgtacgcgc
     5701 cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga cgcaccttgt
     5761 tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga aatattgcta
     5821 actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc gaattctgca
     5881 gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga ggcaagtaat
     5941 tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt ccatcgcggt
     6001 aataaacgcc caggcttcca gccggtaatt tattgtgcac aggtaaacgc atagggacgc
     6061 aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca aaatggcttg
     6121 tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt aataatcagc
     6181 ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag aaccccgaac
     6241 catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc gaatctcttg
     6301 cggatggttc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc tacaggcact
     6361 taacatcaac accaataata aactggcaaa ccggtaaata acgctattac gtttcctgct
     6421 aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa ttgttacctg
     6481 gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa cattatgaac
     6541 taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag acaaaaagga
     6601 gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc ttcatgctta
     6661 tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg gctttacttc
     6721 atccagtctg gtgtatagct attttgccgt tgcgctggcg caggctggtt tgcgggtgat
     6781 catgccggat gcgcccgatc acggtagccg ttttagtggt gacgcagcgc ggcggttaaa
     6841 tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac gtgcggcaat
     6901 agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt cgatgggcgc
     6961 gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg ccagcatgat
     7021 gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga tacctgaaac
     7081 ggcagcacag cagaatgaat tcaataacat tatcgcgccg ctggcagagt gggaagcgac
     7141 aaaccacctg gaacaatttg gggacagacc tctactgctg tggcatggcc tcgacgatga
     7201 cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga cgggacggga
     7261 taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc ctgaggcgtt
     7321 agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc tgaccccttg
     7381 cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgataa gcatatcgat
     7441 ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc tatcaaccag
     7501 taccaccagt ttccctacca cgctcagcat cttctgctct gccattgccg tcagcatatc
     7561 ggttttatac aacccttctg cggtcagtcc tttgccgctg gtaaacatcc agtgcccggc
     7621 atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt tgttgtactg
     7681 tccgcccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg ccagcggtag
     7741 ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca gaaacgcggt
     7801 ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg ccgctttagc
     7861 aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg tccagcgcgg
     7921 gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc cgctttcgtc
     7981 aagtttattg atatcgcgtc gcgcagtggc aggtgaaatt cccagacgct caacgacttt
     8041 ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat gtctttgtgc
     8101 ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg aaatagccag
     8161 gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac gacagattac
     8221 aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa accgcgcgga
     8281 tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc aacctgccag
     8341 ataaacggct tgaagccata cttcagacga tcttttttca tctcccacag cacgcggatc
     8401 tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat aaccactttc
     8461 gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc ggtaataccg
     8521 cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt accgtgcttc
     8581 gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc aggggttttg
     8641 aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc cgctttttga
     8701 tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg aatttcaatg
     8761 tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt cggtacgccc
     8821 cagccaatcc acaaatccac acaggttttc ggtccaatga acggtacgtc atcagcacag
     8881 ttctgcatca cagcagcagc gacgttgacg tcgatatgat cgttgtgatc gtgagtcgcc
     8941 agtaccgcgt cgacctggcg aatcgcaaac ggatcaagaa caaacggggt ggtgcgcagg
     9001 tttggctgca gttttttcac gccagccatg cgctgcattt ggtgaccctg tttcattaac
     9061 gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac gttggtgcca
     9121 ccttccgatt ttaaccagat cccggtgcag ccaagccacc acatcgcaaa tgtgccagga
     9181 gcgacctgtt cttgttcaat ttcttcattc aaccagctac cccactccgg gaaagtgctc
     9241 aggatccagg attcacgggt gatacttttc actttactca tcgccattta ccttcatgat
     9301 agttcaattc gaatcaatat atgattggtt ttgattaatc ctgacactat ttttcaggaa
     9361 ggcaatgacc attttttgac tttttactag ggaagttgtt gttgattttg agtatggaaa
     9421 gatttaatgg aatgtgtaat tcaattaact aaatgaattt aaatgaataa ttgtttcgtt
     9481 gtgtgcatcc cactctatcc atgtggaatt atttgcgggt cgtgtcacat ttaatcataa
     9541 ataatcttgt tgtgattatt tttgaaaatt agagtgagtg cacaacattc cgggtgtgtg
     9601 gaatacccgg ttacctcttc ttcaggagat cgttatggag atcctctaca acatctttac
     9661 cgtgtttttt aaccaggtca tgaccaatgc cccgttgttg ctgggtattg tgacctgtct
     9721 gggctacatc ctactgcgca aaagtgtcag cgttattatt aaaggcacga ttaaaaccat
     9781 aattggtttc atgttgttgc aggcagggtc cggcatcctc accagcacct tcaaaccggt
     9841 ggtggcgaaa atgtccgaag tctacggcat taacggcgca atttccgata cttacgcgtc
     9901 gatgatggca accatcgacc gcatgggcga tgcctatagc tgggtaggtt acgcggtgtt
     9961 gttagcgctg gcgctgaaca tctgttacgt gctgctgcgt cgcattaccg gtattcgtac
    10021 catcatgctg actggtcata tcatgttcca gcaggcgggg ctgattgccg ttacgttgtt
    10081 tattttcggc tactccatgt ggacaaccat tatctgcacg gcgattctgg tttcgctcta
    10141 ctggggcatt acctccaaca tgatgtacaa gccgactcag gaagtgacgg acggctgcgg
    10201 tttctccatc ggtcaccagc aacagtttgc atcatggatt gcctataaag ttgcgccgtt
    10261 cctcggcaaa aaagaggaaa gcgttgaaga cctcaaactg ccaggctggc tgaatatttt
    10321 ccacgacaac atcgtctcca cggcgattgt gatgaccatc ttctttggtg ccattctgct
    10381 ctccttcggt atcgacactg tgcaggcgat ggcaggcaaa gtgaactgga cggtgtacat
    10441 cctgcaaact ggtttctcct tcgcggtggc gatcttcatc atcactcagg gcgtgcgcat
    10501 gtttgtggcg gaactctctg aagcatttaa cggtatctcc cagcgcctga tccctggtgc
    10561 ggttctggcg attgactgtg cggctatcta tagcttcgcg ccgaacgccg tggtttgggg
    10621 ctttatgtgg ggcaccatcg gtcagctgat tgcggttggc atcctggtcg cctgcggttc
    10681 ctcgatcctg attattcccg gctttatccc gatgttcttc tccaacgcca ccatcggcgt
    10741 gttcgctaac cacttcggcg gctggcgtgc ggcgctgaag atatgtctag tgatggggat
    10801 gattgaaatc tttggctgcg tctgggcggt gaaactcacc ggtatgagcg cctggatggg
    10861 catggcggac tggtcgattc tggcaccgcc gatgatgcag ggcttcttct ccatcggtat
    10921 cgcctttatg gccgtcatca ttgtaattgc actggcttat atgttcttcg ctggccgcgc
    10981 actgcgcgca gaagaagatg cagaaaaaca actggcagaa cagtctgctt aataaggagt
    11041 tttgattatg accgtacgta ttctggctgt gtgtggcaac ggacaaggca gttctatgat
    11101 catgaagatg aaagtggacc agtttttaac ccaatcaaac attgaccaca cggtaaatag
    11161 ctgcgcggtt ggcgaataca aaagcgagtt gagcggcgcg gatatcatca tcgcttctac
    11221 gcacattgcg ggcgaaatca ccgtgaccgg caacaaatac gtggttggcg tgcgcaacat
    11281 gctctctcct gccgattttg gcccgaaact gctggaagtg atcaaagcgc atttcccgca
    11341 ggatgtgaag taaggacgcg ccatgaaatt acgtgattcg ctggcggaga ataaatccat
    11401 ccgcctgcag gctgaagctg agacatggca ggaggcagta aaaatcggcg ttgacctgct
    11461 ggtggcggcg gatgttgtcg agccgcgtta ctaccaggcg attctggatg cggttgaaca
    11521 gcatggtccg tactttgtgc tcgcaccagg cctggcaatg ccgcacggtc gcccggaaga
    11581 gggcgttaag aaaaccggtt ttgcgctggt gacgctgaaa aagccgctgg agttcaacca
    11641 cgaagataac gacccggtgg atatcctcat caccatggcg gcggtcgatg ccaacacgca
    11701 ccaggaagtg ggcatcatgc agatcgtcaa cctgtttgaa gatgaagaaa atttcgaccg
    11761 tttacgtgcc tgccgtaccg agcaggaagt actggatctc attgaccgca ccaacgcggc
    11821 agcttaagaa ggaattgaag atgtcattac cgatgttgca agtcgcgctg gacaaccaga
    11881 ctatggatag cgcctacgaa accactcgcc tgattgccga agaagtcgac attatcgaag
    11941 tgggtaccat tctgtgcgtg ggcgaaggcg tgcgtgcggt tcgcgatctg aaagcgctct
    12001 acccgcacaa aatcgtgctg gcagacgcca aaattgccga tgcgggcaaa attctttctc
    12061 gtatgtgctt cgaagccaac gctgactggg tgacggtaat ttgctgtgcg gatatcaaca
    12121 ccgccaaagg cgcgctggac gtagcaaaag agtttaacgg cgacgtgcag atcgaactga
    12181 ccggctactg gacctgggaa caggcgcagc agtggcgtga cgcaggcatt cagcaggtgg
    12241 tttatcaccg cagccgtgac gcgcaggccg caggcgtagc gtggggcgaa gcggacatta
    12301 ccgcgatcaa acgtctttcc gatatgggct tcaaagtcac cgtcaccggt ggcctggcgc
    12361 tggaagatct gccgctgttc aaaggtattc cgattcatgt gtttatcgct ggtcgcagca
    12421 tccgtgatgc cgcttctccg gttgaagctg cacgtcagtt caaacgttcc atcgccgaac
    12481 tgtggggcta aggagcggat atgttgtcca aacaaatccc gcttggcatc tatgaaaaag
    12541 cgctccccgc cggggagtgc tggctggaac gcctgcaact ggcaaaaacg ttgggcttcg
    12601 attttgtcga gatgtcggta gatgaaacgg acgagcgcct gtcgcgcctc gactggagcc
    12661 gcgagcagcg tctggcgctg gtgaatgcga ttgttgaaac cggcgtgcgc gtgccgtcca
    12721 tgtgcctttc tgctcatcgt cgtttcccgc tgggcagtga agatgacgcg gtgcgggcgc
    12781 aggggctgga gattatgcgt aaagctatcc agttcgccca ggacgtcggc attcgcgtga
    12841 tccagctggc gggctatgac gtttactatc aggaagccaa taacgaaacg cgtcgtcgtt
    12901 tccgtgacgg cctgaaagag agcgttgaga tggcaagccg cgcgcaggtg acgctggcga
    12961 tggagatcat ggattatccg ttgatgaact ccatcagcaa ggcgctgggt tacgcgcact
    13021 atctcaacaa cccgtggttc cagctctacc cggatatcgg caacctgtcg gcgtgggaca
    13081 acgatgtgca gatggagttg caggccggaa tcgggcatat cgtcgcggtg catgtgaaag
    13141 acaccaaacc gggcgtcttc aaaaacgtgc catttggcga aggtgtggtg aatttcgaac
    13201 gttgcttcga aacgctcaaa cagagtggct attgcgggcc gtacctgatt gagatgtgga
    13261 gcgaaacggc ggaagacccg gcggcagaag tggtgaaagc gcgtgattgg gtgaaagcgc
    13321 gcatggccaa agcaggcatg gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt
    13381 tgaagccaac atggatctgc cgcgctatgg gctggtgacc tttacctggg gcaacgtcag
    13441 cgctatcgac cgcgaacgcg ggctggtggt gatcaagccc agcggcgttg cctacgaaac
    13501 catgaaagca gacgatatgg tggtggtcga tatgagcggc aaggtgttag aagggaagta
    13561 tcgcccgtct tctgacaccg cgacgcatct tgaactctac cgtcgttacc cgtcgctcgg
    13621 cggcattgtc catacccatt ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat
    13681 cccggcgtta ggcaccacgc acgccgacta cttctttggc gacatcccgt gtacgcgtgg
    13741 attaagcgaa gaagaggtgc agggcgagta tgaactgaac accggcaaag tgattatcga
    13801 aacgctgggt gacgccgagc cgctgcatac gccgggaatt gtggtgtatc agcacgggcc
    13861 gttcgcctgg gggaaagatg cccacgacgc ggtgcataac gcggtggtga tggaagaagt
    13921 ggcgaaaatg gcgtggattg cgcgcagtat taacccacaa ctgaatcaca tcgacagctt
    13981 cctcatgaat aaacacttca tgcgtaagca cggtcctaac gcctattacg ggcagaagta
    14041 agcagtgcgc cccggaatta ttacctcctg cgggatgtaa ttggtctgat tccggggccg
    14101 cttcagttaa aaaatcgtat aaacaacagg tgtttttatg gctatcaatg gattgtttgt
    14161 cagaacggat ccgcaacgtc gccgctatgg cgttgcgtta cttgtgggtg tactttcagg
    14221 aattatttca gcatttgtta aatggggagc ggaagtcccc ttgccaccgc gcacattttc
    14281 cggtggtcgc gatgaattta acccgccgta tatctttcta cgggattatt taggtatcga
    14341 cccaacccaa actgtgtata cattctcgga acatattatc aacccggtga tggtgacgca
    14401 tatcatcttc tcactggttt ttgcgatagg gtattgtatt gtcgcggaaa ttttcccgaa
    14461 agtgaaatta tggcagggga tattagcagg gcttattgtg acagtggcag tccacggtat
    14521 attttgcccg gcgctgaatt taacgccgcc gctcactcaa ttgccttttg acgaatatgc
    14581 ctctgaaatc ctggggcata ttttctggtt ctggataata gaaattatgc gccgcgactt
    14641 acgtaatcgc attactcatg agccagatcc ggaagtcgca atccggtgaa taaccgtata
    14701 aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa tgtttcgttg gttatacctg
    14761 gtgctagcga taaatatccg cgctggcgtg catattgccg ttactccccg gttcccgcat
    14821 cagaattacg tggtagtacg ttgcgccctg cgcatctgtc tcttcattta atgcctgacg
    14881 tgcttcgctt tcagtggcga aattatgatt gatataaatc acgcctaagc tttgcacatt
    14941 atccatgttt ctggcctggt ggttattaat ttcaatggct gcccatgtat ttgcacttag
    15001 caaaagcaca gccagaaggg ctaaaacacg actgaacata gatacctcct cgacggctga
    15061 ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt ttaatcaatg ataaagaagt
    15121 tgatggtgac catttctgat gcagttgttc aaaaaaacac catgatgaag tgtgatgaac
    15181 tccaaatcag cggtttagag gttaattgcg aaaggggaga tttatttcgg ctctgccctt
    15241 gagtttagcg gggcatacaa gtactataac gcgtcatttt tcagccgacc tttaacacgt
    15301 tccttgcctc cccgggattc ggctgaccca gacaggaggc tgaataatcc gtaaggagca
    15361 attcgatgcg tcattacgaa atcgttttta tggtccatcc tgaccagagc gaacaggttc
    15421 cgggcatgat cgagcgctac actgctgcca tcactggtgc agaaggcaag atccaccgtc
    15481 tggaagactg gggccgccgt cagctggctt acccgatcaa caaactgcac aaagcacact
    15541 acgttctgat gaacgttgaa gctccgcagg aagtgatcga tgagctggaa actaccttcc
    15601 gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac caagcacgct gttaccgaag
    15661 catctccgat ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg
    15721 aaaccgctga tgatgctgaa gctggggatt ctgaagagta atttctgatg accaaccgtc
    15781 tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc
    15841 ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc
    15901 aggcatggtg tcaaatgccc gttattgtta gcggacacga aaaccaggcc attactcaca
    15961 gtataacggt cggcagtcgc ataaccgttc aggggttcat ttcatgccac aaggcaaaga
    16021 acggactgag caaaatggtt ttgcatgccg agcagattga attgatagat tctggagact
    16081 agccatatgg cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt
    16141 caagagatcg actataaaga tatcgctacg ctgaaaaact acatcaccga aagcggtaag
    16201 attgtcccaa gccgtatcac cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct
    16261 atcaaacgcg ctcgctacct gtccctgctg ccgtacactg atcgccatca gtaatcggtc
    16321 acggtccatt aatacgactt tgagaggata aggtaatgca agttattctg cttgataaag
    16381 tagcaaacct gggtagcctg ggtgatcagg taaacgttaa agcgggctat gctcgtaact
    16441 tcctggtacc gcagggtaaa gctgttccag ctaccaagaa aaacattgaa ttcttcgaag
    16501 cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct ggcagctgct aatgctcgcg
    16561 ctgagaaaat caatgcactt gaaactgtta ccatcgcgtc taaagctggc gacgaaggta
    16621 aactgttcgg ttccatcggt actcgcgaca tcgctgacgc tgtaactgca gctggcgttg
    16681 aagtggctaa gagcgaagtt cgtctgccga acggcgttct gcgtaccact ggcgaacacg
    16741 aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg
    16801 aataattcgt tattcaacga gacgtaaaaa cgccgaccag tggtcggcgt tttgcttttg
    16861 tagcgagcac ttgcagatat cgataagggc cacaataagc agccctacga agataattac
    16921 ttagctgcct gaatatcagc cagcattggg gcaagggctg ggttgtcctt agcgaagttt
    16981 gcgtacattg gctgtacttt ctcgataaac ggagctttat ctacctggta gactttcacg
    17041 cccattttat ccagttttgc aaattcttct ttctcttcct cgctccacaa gtctttcatg
    17101 tagctcattg attctttagc tgcttttaat ataactgcct gattttcctg gctaagttta
    17161 tcaaacgcag cattgctcat cagaaccaca tcgggaacca tagtgtgttc gtcaaggctg
    17221 aaggattttg ccacttcacc gtgacgcatg gtagtcagcg ccatcacgct gttttctgcc
    17281 atatcgacca caccctgctg taaggctgta tacaactcgc cgtaatccag tggcgtcggg
    17341 ttaccgccca tgactttgat catttcaact gcgccagggc ttggctggac acggactttc
    17401 atgcctttca gatcgtctgg tttcagtact ggtttattgc catagaaact gcgggcgcct
    17461 ccatcataaa aagtcagccc gaaataacct ttgcttttcg ttgagtcgag gatttttctc
    17521 ccgatatcgc cctgcatgac ctgataataa tgatcacgat cgcggaacaa ataaggcagg
    17581 ctaaataatt tataggaatt ttcaaatgct tccatttctg ccgcattggt ttttaccagg
    17641 ggaatagcgc cagaacgaat cagctccatt gtttctcgtt gagtacccaa tgttccattt
    17701 ggataaatac gaatagtaat atcaccgtta gagtactctt tgctcttttt cgcaaagaac
    17761 tccatcgctt tatgaacagg atgagactta tcctgattat gactcagttt taatgtcact
    17821 gcccaggact ggcttgcgga taacataaga acagcaccga taatgccagc ggataccttt
    17881 attttcatag atgttccttt tctaataaat atggcggcag gtgccgccag tgaatgcgca
    17941 tcagtagccc agtgctcttg gcaatgccat actgatttgc ggaatatagg tcaccattgc
    18001 cagcaccaat accatcacgg catagaatgg cagcataggt ttgataattt tattaatcgg
    18061 taatttagat acactacaac cgacaaataa cacactgcct acaggcggag tacaaatacc
    18121 gatacacatg ttcagtacca tgacaatacc aaagtgaatc gggtccatgc ccagttcggt
    18181 gacaaccggt aaaaatatcg gcgtaaaaat cagtaccgcc ggtgtcatat ccataaaagt
    18241 gccgataatc aacaggataa tattgataat aagcaggatt attattgggt tgtcagaaat
    18301 agtgttaagc aaatcgagaa tcaggaaggg aacgtcagcg tttgacattg cccatgacat
    18361 acccatcgaa gaac
//
LOCUS       DAZFAH010000084        18303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFAH010000084 DAZFAH010000000
VERSION     DAZFAH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.084"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..558
                     /locus_tag="ACHJWB_002690"
                     /pseudo
     CDS             1..558
                     /locus_tag="ACHJWB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896819.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            661..1164
                     /locus_tag="ACHJWB_002691"
     CDS             661..1164
                     /locus_tag="ACHJWB_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4314299.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1231..2688)
                     /gene="nuoN"
                     /locus_tag="ACHJWB_002692"
     CDS             complement(1231..2688)
                     /gene="nuoN"
                     /locus_tag="ACHJWB_002692"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4314300.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALLYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2695..4224)
                     /gene="nuoM"
                     /locus_tag="ACHJWB_002693"
     CDS             complement(2695..4224)
                     /gene="nuoM"
                     /locus_tag="ACHJWB_002693"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4314301.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4388..6229)
                     /gene="nuoL"
                     /locus_tag="ACHJWB_002694"
     CDS             complement(4388..6229)
                     /gene="nuoL"
                     /locus_tag="ACHJWB_002694"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4314302.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6226..6528)
                     /gene="nuoK"
                     /locus_tag="ACHJWB_002695"
     CDS             complement(6226..6528)
                     /gene="nuoK"
                     /locus_tag="ACHJWB_002695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4314303.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6525..7079)
                     /gene="nuoJ"
                     /locus_tag="ACHJWB_002696"
     CDS             complement(6525..7079)
                     /gene="nuoJ"
                     /locus_tag="ACHJWB_002696"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4314304.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7091..7633)
                     /gene="nuoI"
                     /locus_tag="ACHJWB_002697"
     CDS             complement(7091..7633)
                     /gene="nuoI"
                     /locus_tag="ACHJWB_002697"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4314305.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7648..8625)
                     /gene="nuoH"
                     /locus_tag="ACHJWB_002698"
     CDS             complement(7648..8625)
                     /gene="nuoH"
                     /locus_tag="ACHJWB_002698"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4314306.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8622..11348)
                     /gene="nuoG"
                     /locus_tag="ACHJWB_002699"
     CDS             complement(8622..11348)
                     /gene="nuoG"
                     /locus_tag="ACHJWB_002699"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4314307.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11401..12738)
                     /gene="nuoF"
                     /locus_tag="ACHJWB_002700"
     CDS             complement(11401..12738)
                     /gene="nuoF"
                     /locus_tag="ACHJWB_002700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4314308.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHIGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12735..13235)
                     /gene="nuoE"
                     /locus_tag="ACHJWB_002701"
     CDS             complement(12735..13235)
                     /gene="nuoE"
                     /locus_tag="ACHJWB_002701"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4314309.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13238..15040)
                     /gene="nuoC"
                     /locus_tag="ACHJWB_002702"
     CDS             complement(13238..15040)
                     /gene="nuoC"
                     /locus_tag="ACHJWB_002702"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4314310.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15134..15796)
                     /gene="nuoB"
                     /locus_tag="ACHJWB_002703"
     CDS             complement(15134..15796)
                     /gene="nuoB"
                     /locus_tag="ACHJWB_002703"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4314311.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15812..16255)
                     /gene="nuoA"
                     /locus_tag="ACHJWB_002704"
     CDS             complement(15812..16255)
                     /gene="nuoA"
                     /locus_tag="ACHJWB_002704"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4314312.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16884..17822)
                     /gene="lrhA"
                     /locus_tag="ACHJWB_002705"
     CDS             complement(16884..17822)
                     /gene="lrhA"
                     /locus_tag="ACHJWB_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4314313.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4568 a         5078 c         4723 g         3934 t
ORIGIN      
        1 ctgcattatc gccaacgccc cgacacgtaa atcctgagca attagaaaaa acagttcgtt
       61 atcttacaca aactgtgcat ccacgtaatg ccgacaatat tgataatctg aataggtccg
      121 tagagtatat aaaagagttc tttgtcagta gcggtgccag agttaccgcg cagggcgtcc
      181 ccgattggct attacgactc tgcgcctggt agccaggatt acccttaccc gcaatgtcct
      241 ggctttatcc cgatcggggg gactttattg cctggtcggc agaatacagg atatcaacgc
      301 cgttcgtcag gtaaaaacgg cattgttatc atcccgggat ttatctgttt attctatgaa
      361 ttccccatgg tttattcccg gtattgattt ctctgaccac ctgaattatt ggcaacacga
      421 tattcccgcc gtaatgatta ctgacaccgc tttttatcgt aataaacaat accacttgcc
      481 cggtgataca gcagacagat tgaattatca gaaaatggct cagatggtgt tacaaccttg
      541 ttacacaaca gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca
      601 ttttattttc cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa
      661 atgggaatga ttggttattt tgcagaaatt gactcagaaa agattaacca actgcttgag
      721 tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg
      781 gatattgata aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat
      841 cccgcgaaga atgatccgct gagtcgtgcc gtgcttggtg aacacagcct cgaagatggc
      901 atagatggct tcctgggatt gacctggaat caggagttag ccgcgacaat cgaccgactg
      961 gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg
     1021 gagatttatc cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcttcgatc
     1081 atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat
     1141 gcgctgacgg ttattgttgg gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt
     1201 cggcggcttt ctgacttaca aactaacaga ttacatcagc ggcattgcta accgcacaat
     1261 actaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag aaatcagcac
     1321 cacgataccg cccgcgctgt actgccagtt tgacggtgca tcgcgccccg gttgttccgg
     1381 tgcgtgcagg tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc
     1441 gacaaccacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta
     1501 gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt
     1561 catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc
     1621 agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct
     1681 gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccagtttg
     1741 cagcgcaatc agcgccacca gtaaatagcc gaggtgagag atagatgagt aaccgagcag
     1801 acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg aggcaaaggc
     1861 gataatcgcc agcaccacgc gaattgcttc gctgtcaccc accggtgcgt agaggaacag
     1921 acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg
     1981 cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt
     2041 gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct cgttgagcat
     2101 accgtcgccg aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacag
     2161 cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat
     2221 actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa
     2281 agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg ccagcaggat
     2341 cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc
     2401 ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt
     2461 gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg
     2521 gccaacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt
     2581 gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac
     2641 gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg
     2701 ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga
     2761 ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag
     2821 gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt
     2881 cgctttaccg aagtaagcgc gatgtaacat cgccagcgaa taaacagatg caaagaccag
     2941 cccaaaggta gagataacgg taatcaccgg tacaacctgg aagctgccga acagaatcat
     3001 aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag
     3061 cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg
     3121 ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg ccgacaaacc
     3181 gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagatagc
     3241 aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt
     3301 ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc acatagcaat
     3361 tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta
     3421 agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg gagcctggga
     3481 gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat
     3541 gaagaagccc agcattaaca ggtattccac accactggac attggcgtat tcagcagctc
     3601 ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat
     3661 ggcaatcaac atcaccaggc cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt
     3721 gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg
     3781 caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc
     3841 gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat
     3901 ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca cgaccatcag
     3961 cagcgacagc ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat
     4021 gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg
     4081 caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg
     4141 tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat
     4201 taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac
     4261 cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat
     4321 gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca atcccacaat
     4381 ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg
     4441 atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta cctgcaaagc
     4501 gggaaaggac agccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg
     4561 caatacccag gaacggcttg acgaacactt tgtcatacag ccagtcaaat ccccaggcgt
     4621 tgtaccacca ggtgcccagc agacggcccg gcgcactgtt ggcgatggag gtcaccagag
     4681 tacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag
     4741 aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc
     4801 cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca
     4861 gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga
     4921 agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg
     4981 ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga
     5041 agaagcccgc agtgaccagc ggtagtgccg acagtgctgc gccgcccacc aggaagcaga
     5101 gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat
     5161 ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag
     5221 tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc
     5281 caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg
     5341 caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg
     5401 gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg
     5461 ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg
     5521 tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc
     5581 acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga
     5641 agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac
     5701 ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga tcggtgtaat
     5761 agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca
     5821 gcaggttatc ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga
     5881 agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga
     5941 aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa
     6001 aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg tatgactgct
     6061 cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag
     6121 agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca
     6181 ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc
     6241 acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag
     6301 gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc
     6361 ggtctgaccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat
     6421 ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag
     6481 aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc
     6541 gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca
     6601 cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc cagttccacc
     6661 gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg
     6721 tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc
     6781 gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcaatt
     6841 tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat aatagcaccc
     6901 gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg
     6961 gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg
     7021 atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt
     7081 gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt
     7141 cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt
     7201 atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc tggcgcttgt
     7261 attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc
     7321 acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccggg taccagcgac
     7381 cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcgcaga
     7441 ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac
     7501 gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga
     7561 acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt
     7621 cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt
     7681 taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg
     7741 gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc
     7801 ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa
     7861 cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccta cgaagaacag
     7921 accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc
     7981 cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa aggtaataaa
     8041 accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga tgtcggtcat
     8101 gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta
     8161 gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt tgttacttga
     8221 ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat
     8281 cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg caaaggccag
     8341 cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt
     8401 cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc
     8461 gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc
     8521 gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca ccgctttgag
     8581 gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg
     8641 agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc catcggcaag
     8701 cccacctgcc cagccgtcag cccttcagcg atttcaaccg gcagcgtgac cgtgttgcca
     8761 tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc atccgctggg
     8821 ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat
     8881 tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc ctgcggctgg
     8941 aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc aaacagacgc
     9001 acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc
     9061 tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcagtcggc
     9121 tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc
     9181 tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg
     9241 gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacctgcc
     9301 agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact
     9361 tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc cagcatgaca
     9421 gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc gcggccttcg
     9481 ttgttgatca ccgtaccgtc gctttcagca aagctggcag cagaaagtac cagatgggcg
     9541 ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc
     9601 gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag caccaccacc
     9661 gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc gccacccata
     9721 atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg
     9781 cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg
     9841 ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg
     9901 attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt atccagcgca
     9961 tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc
    10021 gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt
    10081 tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt
    10141 tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc gccggtctgg
    10201 gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga
    10261 gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg
    10321 tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt
    10381 tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct
    10441 gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt
    10501 actggttgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg accacggtcg
    10561 cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg
    10621 taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca
    10681 aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg
    10741 gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg
    10801 cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc gtcagcgtaa
    10861 tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg
    10921 aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg
    10981 tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccctcttc acataccgga
    11041 cagtcgtgcg ggtggttggt catcaaccac tcgaccacgc tttcacggaa ctgtttcgct
    11101 tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc
    11161 aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca
    11221 ccgacacttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga
    11281 caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt
    11341 gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg
    11401 ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa
    11461 cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg
    11521 ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag
    11581 ttgctcaagt gtttcgatat cgcccggctg accttcacca cgctccagcg cacgcagaat
    11641 tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc
    11701 gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat
    11761 cgccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag
    11821 gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgccg cctggctgcc aggctttgaa
    11881 tttcagaccg tcacgcatac caccggcgta atcttcgagg atctcgcgcg cggtagtacc
    11941 aaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca tcagcttggt
    12001 gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc
    12061 cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga
    12121 ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat
    12181 taacgctgtt tcttccccgc agatgtagcg ccctgccccg atatggacga acagttcgaa
    12241 gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat
    12301 ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg
    12361 gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg
    12421 ctccatcagc aggcggtctt tataggtgcc aggctccatt tcatcggcgt tacacagcag
    12481 gtaacggatg ttcatggatt cgtctttcgg catcaggctc catttcaggc cggtagaaaa
    12541 gcccgcgcca ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg
    12601 agacagcccg gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc gatattcgtc
    12661 cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt
    12721 acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga
    12781 tgcgcgtgag tatcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag
    12841 caagttggca gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt gatgtttagc
    12901 tttttctcga gcgccgcctg aataccctgg taaccgttga tatgacagac cacgctgtcg
    12961 caataacgga tcacatggcg gccaaccggc tggcggaaga tctggctgta gaacgttgcc
    13021 acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca
    13081 tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc
    13141 gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca
    13201 aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa
    13261 caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg
    13321 ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg
    13381 tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga
    13441 aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg
    13501 tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt
    13561 tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca
    13621 gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga
    13681 tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga
    13741 tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat
    13801 aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt
    13861 aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccgc
    13921 gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg
    13981 tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg
    14041 tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga
    14101 acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca
    14161 gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac
    14221 ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac
    14281 ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa
    14341 cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt
    14401 cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca
    14461 tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg
    14521 ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt
    14581 tcgggtgacc gtcgaatgta atgccaaaca gatcccaggt ttcacgctca taccagttag
    14641 cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct
    14701 tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg
    14761 cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca
    14821 gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac
    14881 gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc
    14941 caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcgcgg gtctgccagg
    15001 cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac
    15061 tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc
    15121 cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac
    15181 cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca
    15241 cccaggagag cgggcgacgt tctttgccga tagattcctg caacagcatc agtgcctgca
    15301 tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat
    15361 cgacgccctg cacaacggaa taaatatcgt acataccgcc ggagttggca caggccccca
    15421 ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca
    15481 ttttggtaaa gcaggttcct gccaccacca tcaggtcagc ctgacgcggc gaagcacgca
    15541 atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa
    15601 cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa
    15661 ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg
    15721 ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg
    15781 tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg
    15841 tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac
    15901 gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc
    15961 ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt
    16021 cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg
    16081 agccgaccga gtcgataccg gattcaaacg gtacgttttt cgacctcgcg cgtgcgcgac
    16141 cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa
    16201 agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt
    16261 actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt
    16321 catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt
    16381 gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca
    16441 ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca gaaccttgac
    16501 gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac
    16561 gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga
    16621 tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt
    16681 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgtacgg ggcgtgaatc
    16741 tctggtgatt gaaaaatgaa cagttttgtg cgttctgcac gatacgagga aggcttttta
    16801 ctaaaaaaag ccgctggagt ttaaaatccc cccagcggct cgtttttaca ctattgttcc
    16861 tggaattatc tatcgtccgt cgattactcg atatcccttt caattaacaa tgagtcatct
    16921 ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc catcgcctga
    16981 tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa atattcggta
    17041 tcgggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtctgg actcatcatc
    17101 tcgaccggcc ttgccgtcac gccaagaccg gcttttactg ccgcacgaac tgccggaagc
    17161 gtcgaggcga cataagccag tcgccaggga atatctgctt tattaagcgt cgccagcacc
    17221 atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc gcctttttgc
    17281 aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt caatgcctta
    17341 aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc cagcatttct
    17401 gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata aaccgaactc
    17461 acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc accgatagtt
    17521 aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt aaaacgcagg
    17581 attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt gttgcgaccg
    17641 tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg ctgacttacg
    17701 gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag atcggcaaca
    17761 gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt tgcacttatc
    17821 atatattatc acttactggc ggctcatact gagctggtta atgctgtgca cacacaaaca
    17881 agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg acgaaagtaa
    17941 aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa tgcctgaagt
    18001 agatcacaga atatatcttc agggatcgca tatctattaa gttactcact cttttctatt
    18061 tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact ctcgtgcagc
    18121 atcgctggtc atacgcgaat acgtacaaac agcaatggtg aggcggcatc aagagcaggg
    18181 gtccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg gtcattgcga
    18241 attatctgat gcaaagttat gttatgttag gcaaagtaat cttctattat taataagcac
    18301 atc
//
LOCUS       DAZFAH010000085        18255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFAH010000085 DAZFAH010000000
VERSION     DAZFAH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.085"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..83
                     /gene="tnaC"
                     /locus_tag="ACHJWB_002706"
     CDS             9..83
                     /gene="tnaC"
                     /locus_tag="ACHJWB_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4314314.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            304..1719
                     /gene="tnaA"
                     /locus_tag="ACHJWB_002707"
     CDS             304..1719
                     /gene="tnaA"
                     /locus_tag="ACHJWB_002707"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4314315.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1810..3057
                     /gene="tnaB"
                     /locus_tag="ACHJWB_002708"
     CDS             1810..3057
                     /gene="tnaB"
                     /locus_tag="ACHJWB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4314316.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3189..4364
                     /gene="mdtL"
                     /locus_tag="ACHJWB_002709"
     CDS             3189..4364
                     /gene="mdtL"
                     /locus_tag="ACHJWB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4314317.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4339..5298
                     /gene="yidZ"
                     /locus_tag="ACHJWB_002710"
     CDS             4339..5298
                     /gene="yidZ"
                     /locus_tag="ACHJWB_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4314318.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5455..6204
                     /gene="yieE"
                     /locus_tag="ACHJWB_002711"
     CDS             5455..6204
                     /gene="yieE"
                     /locus_tag="ACHJWB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4314319.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6226..6792
                     /gene="yieF"
                     /locus_tag="ACHJWB_002712"
                     /gene_synonym="chrR"
     CDS             6226..6792
                     /gene="yieF"
                     /locus_tag="ACHJWB_002712"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4314320.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6846..8183)
                     /gene="adeP"
                     /locus_tag="ACHJWB_002713"
     CDS             complement(6846..8183)
                     /gene="adeP"
                     /locus_tag="ACHJWB_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4314321.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8350..9015
                     /gene="yieH"
                     /locus_tag="ACHJWB_002714"
     CDS             8350..9015
                     /gene="yieH"
                     /locus_tag="ACHJWB_002714"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4314322.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9082..9555
                     /gene="cbrB"
                     /locus_tag="ACHJWB_002715"
     CDS             9082..9555
                     /gene="cbrB"
                     /locus_tag="ACHJWB_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO4314323.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9604..10191
                     /gene="cbrC"
                     /locus_tag="ACHJWB_002716"
     CDS             9604..10191
                     /gene="cbrC"
                     /locus_tag="ACHJWB_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO4314324.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVDWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10253..10975)
                     /gene="yieK"
                     /locus_tag="ACHJWB_002717"
     CDS             complement(10253..10975)
                     /gene="yieK"
                     /locus_tag="ACHJWB_002717"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4314325.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10990..12159)
                     /gene="yieL"
                     /locus_tag="ACHJWB_002718"
     CDS             complement(10990..12159)
                     /gene="yieL"
                     /locus_tag="ACHJWB_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4314326.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12186..13802)
                     /gene="bglH"
                     /locus_tag="ACHJWB_002719"
     CDS             complement(12186..13802)
                     /gene="bglH"
                     /locus_tag="ACHJWB_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO4314327.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13888..15282)
                     /gene="bglB"
                     /locus_tag="ACHJWB_002720"
     CDS             complement(13888..15282)
                     /gene="bglB"
                     /locus_tag="ACHJWB_002720"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO4314328.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15301..17178)
                     /gene="bglF"
                     /locus_tag="ACHJWB_002721"
     CDS             complement(15301..17178)
                     /gene="bglF"
                     /locus_tag="ACHJWB_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO4314329.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17312..18148)
                     /gene="bglG"
                     /locus_tag="ACHJWB_002722"
     CDS             complement(17312..18148)
                     /gene="bglG"
                     /locus_tag="ACHJWB_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO4314330.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4612 a         4554 c         4305 g         4784 t
ORIGIN      
        1 agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat atcgacaata
       61 agattgtcga tcaccgccct taatttaccc ttctgtagcc atcaccagag ccaaaccgat
      121 tagattcaat gtgaacgatt agtttgctat atcttaattt tgccttttgc aaaggccatc
      181 tctcgtttat ttacttattt tagtaaatga tggtgcttgt atatatacat ggcgaattaa
      241 tggatattgc agatgtaata ttcacaggga tcactgtaat taaaataaat gaaggattat
      301 gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat tgagccagta
      361 aaacgtacta ctcgcgctta tcgtgaagaa gcaattatta aatccggtat gaacccgttc
      421 ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac cggggcggta
      481 acccaaagta tgcaggcagc gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc
      541 tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatatac tattccgact
      601 caccagggcc gtggcgcaga gcaaatctat attccggtac tgattaaaaa acgcgagcag
      661 gaaaaaggcc tggatcgcag caaaatggtg gcattctcta actatttctt tgataccacg
      721 cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa agaagccttc
      781 gatacgggcg tgcgttacga ctttaaaggc aactttgacc tcgaaggatt agaacgcggt
      841 attgaagaag ttggcccgaa taacgtgccg tatatcgttg caaccatcac cagtaactct
      901 gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa
      961 tacgatattc cggtggtaat ggactccgca cgctttgctg aaaacgccta tttcatcaag
     1021 cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga aacctacaaa
     1081 tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat gggcggcttg
     1141 ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac cctttgcgtg
     1201 gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg
     1261 gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc gcaggtacag
     1321 tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg cggtcacgcg
     1381 gcattcgttg atgccggtaa actgctgccg catatcccgg cagaccagtt cccggcacag
     1441 gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat tggctctttc
     1501 ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga actgctgcgt
     1561 ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat tgaagccttt
     1621 aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa
     1681 gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca gagtggctat
     1741 aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc ttcctctaaa
     1801 ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg tgttatggtt
     1861 atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct tgccggtgcc
     1921 tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct tcattccggg
     1981 ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa caccatcacc
     2041 aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc
     2101 tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga aacgatatca
     2161 atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc cattttcgtt
     2221 gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt gttcctcggg
     2281 ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt cgattactcc
     2341 atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta tatttttatg
     2401 gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag cctgattatt
     2461 tgctatggca aacgcaaaga taagctaatc aaaagcgtgg tcttcggttc gctgctggcg
     2521 ctggtgattt atctcttctg gctctattgc acgatgggga atatcccgcg agaaagcttt
     2581 aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc
     2641 aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc tgttgccagt
     2701 tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt taagattgat
     2761 aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg
     2821 tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct gtgcgccacc
     2881 atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat
     2941 cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct ctttggtata
     3001 accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc
     3061 cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt atgtccaaat
     3121 ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact atcaggacgc
     3181 tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt tatcccgccg
     3241 ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag
     3301 cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg atgttatttg
     3361 ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacctggc gcggcgctat
     3421 ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt cttgcaggcc
     3481 gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc attttgcgcg
     3541 acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt attacctgca
     3601 tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc ccgtggcaga
     3661 gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct ttgtttattt
     3721 taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa aatagcgagt
     3781 cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc gtttcggtga
     3841 tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt tttgagcgcg
     3901 gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt tcattctcca
     3961 cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg caggtgttat
     4021 tccttgcggc ggggatcacc cttgccgttt caccttccca tgcggtttct ctgtttggta
     4081 tcacgctgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt caggcgttag
     4141 gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg caggtttgcg
     4201 gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg aatatgctga
     4261 tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc gtcgcgcctg
     4321 gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc
     4381 tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg
     4441 accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga cgacccgctc
     4501 tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat
     4561 ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca ccagacaccg
     4621 cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg
     4681 ctgtcgaaac ggatctacca acgttacccg caagcgacca tcaaactacg taactgggat
     4741 tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc cggtcgcgaa
     4801 agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga ttatgaagtg
     4861 ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact gcatgaaacg
     4921 tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga acagagcgac
     4981 acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat tgctatgagc
     5041 ctgccggaat tcgagcagtc actgtttatg gcagcgcaac ccgacaatct gctactggcg
     5101 accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt ggttgctctt
     5161 cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac cctgctgtgg
     5221 cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat taaaaacctt
     5281 tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca ataaaatgta
     5341 aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct gtccatcatt
     5401 ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg aaaaatggcg
     5461 actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg tctcccctcc
     5521 cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt tctggcgtcc
     5581 agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt gccggaaatc
     5641 gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc gttttctatt
     5701 tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat
     5761 atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga taaccacacc
     5821 ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga agcgcgggcg
     5881 cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat
     5941 ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca tgtaaatcat
     6001 gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt cacgcccgcg
     6061 attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag cctgccggat
     6121 attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca gctctctacc
     6181 gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc tgaaaaattg
     6241 caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt
     6301 accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc cattgccgac
     6361 attcccttgt atgacgctga cgtacagcag gaagacggtt ttcctgcaac ggttgaagct
     6421 ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga atataactac
     6481 tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc ggatcaaccg
     6541 ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc
     6601 tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat gaacaagccg
     6661 gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga agtgattgat
     6721 cagggtactc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga
     6781 gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt aacgcgctat
     6841 cgattttagt gagcgtcgat aaagacaatc ttcaggataa acagcagcgc aacgatgatt
     6901 acgcacgggc ttagatcacg cagacgtccg gtaccgattt tcatcacgca gtaggagata
     6961 aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac ggcggtaata
     7021 aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact tgaggtcatc
     7081 aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat tcccgccagc
     7141 ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt cagaccggta
     7201 cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga agaagtaccg
     7261 ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt catgcgcggg
     7321 aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag cgtaccggag
     7381 gagtcaaaca ggttgaccag catgaaagag aatatcaccc ctgccagccc gaggttaaac
     7441 gacccggcta aatctacatg gcccacgaca gtcattacgc tcggcggcgc agaaacgatg
     7501 ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac gatggaaacc
     7561 agcaccgccg cgtgaatgtt gcgcgaggcc agaatagcga tgatgaagaa gccgaggatc
     7621 cccagcagca cgctgtgaga agtcaggtta ccgatgctga ccagcgtttc cgggttagcg
     7681 acaatcacac ctgcattctt cagtcccatc ataccaatga acagaccgat accgctggtg
     7741 atgcccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac gcggaaaatc
     7801 gtcagtaaca gcagaccaat cgcgccccag aagattgcgc ccatcccgac ttgccacggc
     7861 aagcccatag cctgtacaac gacaaaagcg aagaatgcat tcaggcccat agcgggtgcc
     7921 agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc agcaatcaga
     7981 caggtagtaa cgaagacggc gctggtatcc atgccagcaa cgccaagaat ttgcgggtta
     8041 acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac ttcggtccgt
     8101 gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccttg gccagaagcc
     8161 tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa tccgtcgcta
     8221 tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca ttgtatttgc
     8281 ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa ctaacgataa
     8341 aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac gctggtcgac
     8401 agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg tattacgctc
     8461 gatcctgaag aggtattcaa acgtttcaaa ggtgtaaaac tgtacgaaat tatcgatatt
     8521 gtttcccttg aacatggtgt tacgttagcg aaaacagaag ctgaacacgt ttaccgtgca
     8581 gaagtcgctc ggctgttcga ttcagaactg gaagccatcg aaggggctgg agcgctcctg
     8641 tcagcgatca ctgcgccaat gtgtgtggta tctaacggcc caaataacaa aatgcagcat
     8701 tctatgggca agctgaatat gttgcactac ttcccggata aactgttcag cggctacgat
     8761 attcagcgct ggaagccaga cccggcgtta atgttccatg cggcaaaagc gatgaatgta
     8821 aatgtagaaa actgcattct ggttgatgac tcagttgccg gtgcgcaatc tggtatcgac
     8881 gcaggtatgg aagtgttcta cttctgcgcc gacccgcaca ataagccgat cgttcacccg
     8941 aaagtcacca cctttaccca tctttcgcag ttaccggaac tgtggaaagc gcgtggttgg
     9001 gatattacgg catagttctt cacactccct tcacttaccc cgcttaaatt ggcgctcaaa
     9061 ggtaagtaaa gggtgtttga tatgtctgtt tcacgtcggg taatacatca cggactttat
     9121 tttgcggttt taggaccgtt aattggtgtt ctgtttcttg tcctctacat attcttcgca
     9181 aaagaaccgc tggttctttt ggtaataata caggtacttc cattgtttct cttattgtcg
     9241 attactacgg gagctattcc tgcattgtta accggcgtga tggtcgcctg tctgccggaa
     9301 aagatcgggt cacagaaaaa ttatcgttgt ctggctggtg gcataggtgg cgtcgttatc
     9361 accgagatct attgtgcagt tattgtacat attaagggca tggcttcctc ggagttgttt
     9421 gaaaacattc tttctggtga cagtctcgtt gtccgcatca ttcctgcatt gctggcaggt
     9481 gtggtgatga gcagaatcat tacccgtctg cccggattgg atatttcatg tcctgaaaca
     9541 gactctttaa gttaagcggg atactttatc tttgggctac tcaaaagcag acaggatgtt
     9601 actatgactc aaaatatcag gccgttaccc caattcaaat atcatcccaa gccactggaa
     9661 acaggcgcat ttgaacagga taaaaccgta gagtgcgatt gctgtgaaca acagacgtca
     9721 gtttattact cgggtccctt ttattgcgtt gatgaagttg aacatctctg tccgtggtgt
     9781 attgcggacg gttctgctgc tgaaaagttt gcaggtagtt ttcaggatga tgccagcata
     9841 gaaggtgttg aatttgagta tgatgaagag gacgaatttg ccggtattaa gaacacatat
     9901 cctgatgaaa tgctgaaaga gttggttgaa cgcacgccag gttatcatgg atggcaacag
     9961 gaattctggc ttgcgcattg tggcgatttc tgtgctttta tcggctatgt ggactggaat
    10021 gatataaaag atcgcctcga tgaatttgcc aaccttgaag aagattgtga gaatttcggt
    10081 attagaaatt ctgatctagc taaatgcctg caaaagggtg gtgattgtca gggttatctc
    10141 ttccgctgtc tccactgcgg caagctgaga ctgtggggtg atttttcgta gttatttaaa
    10201 taatgagaac aggccggagc gtaattcata catccggcct tatttcttca gcttaaccga
    10261 gcgctaattc tgcggcagcg gctttatcag cgattaccat cagcgaagga tgcagctgca
    10321 acacggaagc tggaacatct tcggtaaccg gaccttgcag cacatttttc agtgcctgcg
    10381 ctttacctgc gccactgaca attatcagca agtttttcgc cgccatgatg cttttcggcc
    10441 ccatcgtcac gtagctgtca ggcactaacg aaaagtcgcc gcccaactca ccatgcgcca
    10501 caatatcaac catctctccc tggatcggga actccacggt ttgctcatgg aaatgggtcg
    10561 tattcggcaa attgccacaa aaatgaccat ctgcacctaa tcccagcacc accaaatcca
    10621 ggccgccttc acgcgccagt ttctgatcat gctcgcggta gttatcaata gtgagcttct
    10681 ggatattctc ttctttgatc cccgcagggg tgaaaaacag attacgcaga ttggtaatcg
    10741 ttacaccttc tccctctttg ccgcgaaagg gaatttcatc gaaattatag aaatagcagt
    10801 tatcgtacca gggcttacct ttaaccaggg tggtgaggta ttcatacatg cctttgggcg
    10861 tgctaccggc ggtaattgcc aggttaacac gacgcgtctt cgacatataa ccaagcagat
    10921 ggtgtgccgc gacacggctc atttcctggt aatcttcggt aatgattaat ttcatcttag
    10981 tgccttattt tatttaaata atttctgtac gaaggctgca taggcaggcc gccagacatc
    11041 catttcatgg ttcaggccgg gatattcctg gtaatcaaac ttaatttttt tctgctcaag
    11101 ctcagttttc agcccggcga tatccttgcc ggttacgaca tctttatcac caacgaccac
    11161 agtaaaatta cgtagttgct ggttaatagc tgccggatcg ttcagccggg ccgcgacacc
    11221 ttcatccggt acggttgtcg tggtaacacc actgaatgtg gccagccagc caaagctttc
    11281 cagatgattc attccggaaa ccagcgcctg gtacccgcct tgtgaaagcc ctgccagcgc
    11341 gcgaccatcg gcatctttac ggacattaaa acgcttgcta atcagcggga taatatcgtt
    11401 catcagttcg cgatctgccg ctttagcatt cagcggataa aagactttac gtctttcctg
    11461 aggcacgaaa tcttcgggaa taatgccctt cgcatcggtt tcggtatccg ggatcaccac
    11521 cagcatcggt ttaattttcc cttcagccag caggttatcc atgatttgcg ggatacgccc
    11581 ctgatcgata gcggaacgtc cggtatcacc aaaaccgtga tagaaataga gcactggcaa
    11641 aggctcgccc atgccggtgt atcctggcgg ggtccagaca tacatctgac gttcagattg
    11701 caatgcgttg gagtggtaag ttatggcgat caaatcacca tgcgcaacag aacgcgtatc
    11761 cagataactg cctggcacca gaatcatact ggagttaacc tggcgctgag gcttagtcat
    11821 tgcggtgcct gtatcaatgc tgcgtacacc atcaacattg aaaaaatact cgtacaggtt
    11881 gcctttcagg ataggtgtgc gccacgacca gactcctgct tcgtctttgg tcatcgggtg
    11941 aatattgtcc ggaaccggac cacccaccac tacagagaca ttttttgccc caggggcaaa
    12001 gtagcgaaag gtcacgctgt tatcggcatt gacctgagtc acatactgtt tcaccgggat
    12061 agtgggtgcc gggctggctg gcatatccgc tgctatggcc cactgagcag aaataccgct
    12121 ggcaatagcc agcgttaaag cagcaatttt tatattcatc gtaatattcc tttcattaag
    12181 ttccattacc accagatttc agcctgggca ccgacagcaa actggtcgtc tttattgtct
    12241 tcgaaggtga agccatccag ttcgttttcc agggctttga tatacgtggc gtagaaacga
    12301 atttccggac gcgaggtcaa catactggta ttgactttaa aggtatggaa gagcgtggtt
    12361 ttataaccag actcattaaa tttattacta ttcgcatctt tgttttgctg ggtgaaatag
    12421 cccagttcaa cacctgtctg gttatattgg tcccaaatat aggccgggcg aacaaccgca
    12481 cgaatagatt cgaaatcaga gtgtgcgcct gtttcgtagc tataaatatc gttaccgaag
    12541 gagtaaacaa tcgcgttagc cacaataaaa tgatcgccga tataggcttc gccctgcgag
    12601 gtcagacgaa ctgctgttcc gccggtgtga tcaccataat aacgaccatt aaaggtggta
    12661 aatggacttg cgccagcata acggccaaag ttactggcga tagagttatt cgcaaccagg
    12721 aaggagaatt cgttgaagcc acctttatca aatttctgcg ttaaagatgt gccgaacatc
    12781 caggtatctt tccagtcata atacccgtta ttatcctgat tatctttttc cgatgcgctt
    12841 tcgtttgccg tgacataacg accactcacc attaaggtcg ctttatccca taacgggata
    12901 tctttatagc gtaaatcaat agtattggta ttaatctgct gtttgttttg caggctgcga
    12961 tcgtaatcat cgacatcttc gcgaaccagc gcgatatcaa ttttacccgg accgactttc
    13021 cagttttcca gacctacacc cgctgcggca tcagtacgct gcgttttcca gtccagcatc
    13081 tggatttcaa ttttcggcgc accgtgttta cccacccaga aatcagcctc tggcgcaaag
    13141 ggcaggaaac ctttggtggt aacgtacata tcggagaact gcataaagtt ctcgccaccg
    13201 gcgttatcgc caaaccagcc ggtagagtac tgctgaccaa cgttaccatc catcatcaca
    13261 acggcatcaa cccgtttgcc gttttcgttg tagacacgtt gttttaactg caaatcaaac
    13321 cagccggagt attcgttacc aaagcggccc agagaaccaa tcgcccatga tttaggtgaa
    13381 ccatgagagg cggtccccca accagaacgg taatagccgt tgtaactaaa gccaatttca
    13441 tctttcacaa acttactgaa atctttcaga gtcatggaag aataaatcga gcctgtctca
    13501 ctggcatttt tctcttcatt tttgaccagt acagcatcag gttttgcggc actactggtc
    13561 gggaacggat tggcggcata cccttgatca ttcgtactta cgctacgatt caccgtcgct
    13621 ggcgtatatt ttttcttctc ttcatcttta tactttttga gttcgctttg cgtttctctt
    13681 aacgcttttt ctaataactc aaggcgttgt tccaccgtta atgattcagc caatgattgt
    13741 gcagaaaagg ctaacggtgc cattaataag atggcagagg taataagatt tcgtctaaac
    13801 ataatatccc tttatggtgc aaagaaagaa ttaacgtatc gcatccagac tgttctgaat
    13861 gcgacgataa ttaaggtgct ttattggtta tttttttaat gacagccccc gcgttttgat
    13921 cacttctgca taccatccga agcttttctt acgcgttctt gtgaggcttc cttcgccatt
    13981 atcatcacga tccacataaa taaagccgta gcgcttagac atttgtgaat gagatgcact
    14041 gactaaatca attggccccc aactggtgta ccccataata tccacaccat cggcaatcgc
    14101 ttcatttacc tgtaccaggt gatcgtttaa ataggcaatt cgataatcgt cctgtatcga
    14161 accatccgct tcaacgctgt cttttgcgcc taatccgttc tcgacaataa atagcggttt
    14221 ttgataacga tcccaaagcg tatttaacaa aacccgtaat ccaaccggat caatttgcca
    14281 cccccactct gaacttttca gatgcggatt ggggatcata ttcagtatgt tgccctgcgc
    14341 atttttatta atgctttcgt cgtgggaaac acaaccggtc atgtaataac taaaagagat
    14401 gaaatctacg gtatgtttta aatcttctgc gtcactttca gtcatctcaa tggtgatatt
    14461 gtggtcgcgg aagaaacgct gcatatagcc gggatactga ccacgcgcct gaacatcacc
    14521 aaagaacatc cagcgccggt tctcttccat ggcctgcaac atatcctgtg gctggcaggt
    14581 gagggggtaa accagcccac cgagaagcat attgccgatt ttcgcttcgg ggagcaggct
    14641 atgacaggct ttaactgccc gcgcactggc aaccagttga tggtggatag cctgataaac
    14701 ttccgcctcg ccactctctt ctgccagccc cacgcccgtg aatggcgcgt gtaacgacat
    14761 gttgatttca ttaaacgtca gccataacgc cactttatgt tggtagcgag taaagaccgt
    14821 gcgggcgtaa tgctcgaagt gatcgatgac cgctcgatta gcccaaccgc cgtagttttt
    14881 caccagccca tatggcattt cgtaatggga taacgttacc agcggcttga tccccgcctg
    14941 cgccatttca tcaaacagcc gatcgtaaaa cgctaacccc gcttcattcg gttcgacttc
    15001 gtcgccctga gggaaaattc gcgcccaggc aatggaaata cgcagacagg tgaagcccat
    15061 ctcggcaaat aacgcgatat cttccgggta acggtgataa aaatcgatgg cgacatcttt
    15121 gatattctct ttccccagga tgcgcggttc catttttccc attacgccat gaggctgtaa
    15181 atctgaggtc gagatccctt tgccatcttc ctgccaggca ccttccacct gattggcagc
    15241 tgttgcgcca ccccaaagaa atgtttctgg aaatgctttc ataattaact ccttttatcg
    15301 ttagcgaatg atggataaca gcggctcacc agcgcttatc tgcgccgtgc cgtggggtaa
    15361 tacgtctgta aaatcatcgc tattactgat taataccggc gtcgtcagat caaatccggc
    15421 ctcgcgaata gcagggatat caaaagaaat cagccgatcg cctgtattga ccttgtcacc
    15481 cacgttgacg tgagcggaaa agaatttgcc gtccagtttt acggtgtcga taccgacatg
    15541 aatcaggatc tccacaccat catctgactc aatgccaata gcgtgtaatg tggcgaacaa
    15601 cgaagcaatt cgacccgcaa ccggagaacg cacttcacca accgagggca gaatggcgat
    15661 acctttaccc aacaggccac tggcaaacgt ggtatcagcg acgtgaatga gcggcacaat
    15721 ctctcccgtc atcggtgaac agataccgcc ctgctcaggt ggtgtaataa cctctggtgt
    15781 tttctcttgc ggggcaccct gcgctggctg acgtttagcg gtgatgaaat gaagcatcac
    15841 cgtaccgaca aatgcgcaac cgatggcaat gacaccgcca ataacgctgg cccagacggt
    15901 gaaatcaatt cccgttgacg ggatagtttg catgaaggtg aaaatacttg gcaaaccaaa
    15961 ggagtacact ttcgtttgcg cgtagccaat gatggtggcc cccaaagccc cactgataca
    16021 ggcgataaca aaggggtact tacgcggcag attaacgcca tataccgctg gttcggtgat
    16081 accaaacagg cccgtcaacg ccgctgatcc cgccaccact tttttctgcg catcgcgttc
    16141 gcagaggaag acgccgagcg ccgccccgac ctgcgccata atggcgggca ttaacagcgg
    16201 gatcatggtg tcgtagccca gcacggtgaa gttattgata cacagcggca ccaggcccca
    16261 gtgcagtccg aacatgacga agatttgcca gaagccgccc attaccgcgc ccgcaaatgc
    16321 aggaaccgcc tgataaagcc agagataacc ggcggcaatc agttcgctta tccaggttga
    16381 tagcggcccc accagcagaa aggtgacggg tgtgataacc atcagacata gcaatggtgt
    16441 gaagaaattt ttgattgccg acggtaacca cgcattaagt cggcgttcca gaatgctgca
    16501 caaccaggca gaaaaaataa tgggaataac cgatgacgag taattcaaca atgtgaccgg
    16561 aatacccagg aaatccagcc ccagctcatc cgctttttgc ccgttctcga aagcagtcag
    16621 aattaatgga tgcactaacg ctccaccaat caccatggca gtaaatggat taccgccgaa
    16681 gcgtttcccc gcggtgtatc ccaggattat cgggaagaac caaaacaagg catcactggc
    16741 gctgaataaa attaaataag taccactttg ttcggtcgtc cactgaaaag tgagcgccag
    16801 agccagcata cctttcagga tcccggttgc cgccatcaaa ccgatcagag gcgtaaaaat
    16861 acctgaaata acataaacaa agcggtttag cagattacct ttatcatcat tttccggtgc
    16921 ctgttgcgct ttttcgtcaa ggcctgccac actgttaacc gccaggaaga catcggccac
    16981 atggttacct atgaccacct gaaactggcc accgctttcc accaccataa taataccggg
    17041 ggtctttttc agtacctctg cttgcgcttt gctttcatcc tttaatttaa aacgtaatcg
    17101 cgttgcgcaa tgcatcagac tcacaatgtt atctgcgccc ccgactcctg cgactatttt
    17161 tctggctaac tccgtcataa cttgccctct accgctttgc ggcaaaactc caaaaaaaaa
    17221 cctgaaaaaa acggcctgac gtgaatcaag caattttttt caggttttgc ccgcttagtg
    17281 cggtaacaat cctttactca gtaataatat ttcagtgttc tttgcgcacg cgctctatat
    17341 ttatggctaa aaacataatc tctgcgggtg aaattttacg ttgatactgc aaaccaataa
    17401 aaatggcgat ccgttccgca cattgccatg cttgcgggta attttgtttt actgcttgtt
    17461 gtaatgattc atcactatcg ttaattgaag catgttcaag aatacgccag gataaaaact
    17521 tcagatgtgt aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact
    17581 gaaattttat taattgcagc atttcgcgca ttaactgcgt gacacctgca acatcctcca
    17641 tatttccgct catttgggca ctgaccagat gcatggcaat aaagcccact tcatctttcg
    17701 gtaactgcac gcccaaccgt ttatcaataa tggttaacgc ttcttcccct agctggaact
    17761 ctttcgggta aagccgctgg atatcccaca gcaacgggtt gggcagcaac acgttttgct
    17821 gaaagcgttt aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgta
    17881 attttcccaa gcgctcctgc gctaaagaga taatacgatc acaggttgcc atcacctcaa
    17941 gaggaatatg acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat
    18001 actctttttc tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc
    18061 cgcgccccat gacaactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt
    18121 tgagaatttt ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaacc
    18181 cgacttcacc agtattctct ggttatgtca ggttttgcct gcgaatgcag taacaatcca
    18241 gtcatttatt aatgg
//
LOCUS       DAZFAH010000086        18092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFAH010000086 DAZFAH010000000
VERSION     DAZFAH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.086"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1149
                     /gene="yjgN"
                     /locus_tag="ACHJWB_002723"
     CDS             <1..1149
                     /gene="yjgN"
                     /locus_tag="ACHJWB_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO4314331.1"
                     /translation="FHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANMEV
                     NGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLLLLVLLIFMAAKG
                     LRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSV
                     IISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAF
                     LALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAV
                     STSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLK
                     IWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
     gene            complement(1205..4060)
                     /gene="valS"
                     /locus_tag="ACHJWB_002724"
     CDS             complement(1205..4060)
                     /gene="valS"
                     /locus_tag="ACHJWB_002724"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4314332.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(4060..4503)
                     /gene="holC"
                     /locus_tag="ACHJWB_002725"
     CDS             complement(4060..4503)
                     /gene="holC"
                     /locus_tag="ACHJWB_002725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4314333.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4637..6148)
                     /gene="pepA"
                     /locus_tag="ACHJWB_002726"
     CDS             complement(4637..6148)
                     /gene="pepA"
                     /locus_tag="ACHJWB_002726"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4314334.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            6302..6352
                     /gene="ytgA"
                     /locus_tag="ACHJWB_002727"
     CDS             6302..6352
                     /gene="ytgA"
                     /locus_tag="ACHJWB_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4314335.1"
                     /translation="MSIIILATISTTQENK"
     gene            6415..7515
                     /gene="lptF"
                     /locus_tag="ACHJWB_002728"
     CDS             6415..7515
                     /gene="lptF"
                     /locus_tag="ACHJWB_002728"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4314336.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7515..8597
                     /gene="lptG"
                     /locus_tag="ACHJWB_002729"
     CDS             7515..8597
                     /gene="lptG"
                     /locus_tag="ACHJWB_002729"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4314337.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8716..10218)
                     /gene="yjgR"
                     /locus_tag="ACHJWB_002730"
     CDS             complement(8716..10218)
                     /gene="yjgR"
                     /locus_tag="ACHJWB_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4314338.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(10296..11294)
                     /gene="idnR"
                     /locus_tag="ACHJWB_002731"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(10296..11294)
                     /gene="idnR"
                     /locus_tag="ACHJWB_002731"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO4314339.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(11361..12680)
                     /gene="idnT"
                     /locus_tag="ACHJWB_002732"
     CDS             complement(11361..12680)
                     /gene="idnT"
                     /locus_tag="ACHJWB_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO4314340.1"
                     /translation="MPLIIIAAGVALLLILMFGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12745..13509)
                     /gene="idnO"
                     /locus_tag="ACHJWB_002733"
     CDS             complement(12745..13509)
                     /gene="idnO"
                     /locus_tag="ACHJWB_002733"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO4314341.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13533..14564)
                     /gene="idnD"
                     /locus_tag="ACHJWB_002734"
     CDS             complement(13533..14564)
                     /gene="idnD"
                     /locus_tag="ACHJWB_002734"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO4314342.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14780..15343
                     /gene="idnK"
                     /locus_tag="ACHJWB_002735"
     CDS             14780..15343
                     /gene="idnK"
                     /locus_tag="ACHJWB_002735"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4314343.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(15347..16366)
                     /gene="ahr"
                     /locus_tag="ACHJWB_002736"
     CDS             complement(15347..16366)
                     /gene="ahr"
                     /locus_tag="ACHJWB_002736"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4314344.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQHVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            16562..16646
                     /locus_tag="ACHJWB_002737"
     tRNA            16562..16646
                     /locus_tag="ACHJWB_002737"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16596..16598,aa:Leu,seq:caa)
     gene            16833..>18092
                     /locus_tag="ACHJWB_002738"
     CDS             16833..>18092
                     /locus_tag="ACHJWB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4314345.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGIYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGSNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEISGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASYNRQHRPALELKRLPELLTKIDS
                     YTGRPLTRWATELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFCGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADYLDANREKGICPFDYAKINNPLK"
BASE COUNT         4372 a         4603 c         4501 g         4616 t
ORIGIN      
        1 tttcatggta caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt
       61 ataacgctag gtatctattt gccatgggca ttaatgaaat gtaagcgtta tctctatgct
      121 aatatggaag ttaacggaca acgattttct tatggaatta ctggtgggaa tgtttttgtt
      181 agttgtcttg tttttgtttt ttgctatttc gcaatcttaa tgacagtgtc agcagatatg
      241 ccacttgttg gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca
      301 aaaggactgc gttatcaggc cttgatgacc agtctcaacg gcgtaagatt tagttttaat
      361 tgctctatga aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg
      421 atggggactg ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt
      481 attatatctg tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt
      541 actttatata gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt
      601 ttcaaggtga aattagatac tacgtattgt ataaaatatg ccattctcgc atttttagct
      661 ttattacctt ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgcgtat
      721 gatagttctg tgtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgcaa
      781 cgtaaaatga taatcgcgca gttaatctat tattttggga ttgctgttag caccagttat
      841 ttaacggtgt cgttgcgaaa tcattttatg agcaacctgt cactgaatga tgggcgtatt
      901 cgttttcgct caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg
      961 atatccggga ttacgggcgg tctggcttat ccactgctga aaatatggat gattgactgg
     1021 caggcaaaaa atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa
     1081 gaacaaccag ataaaggctt cttagccagg atttcacggg gaattatgcc ttctttacca
     1141 tttctgtaag agataaaaaa ggccggagca tgctccggcc ttcgttttca ttactgtgtt
     1201 ttgattacag cgcggcgata acagcctgct gttcaatcag cttcgctttc gcttctgcat
     1261 agccttccag cttttcacgc tctttcgcga tgaccgcttc cggtgcgcgg gcgacaaagc
     1321 cttcattcgc cagcttgttc tcgatacggc tgatttcacc ttcaatcttc gccacttctt
     1381 tcgccagacg cgccagctca tcttctttgt tgatgaggcc agccatcggg atcagcagct
     1441 ctgcaccgtc gatgatctta gtaacggaaa ccggaccttt gtcatcggca ggcagcacgg
     1501 tgatactttc cagacgcgcc agcgtttgca ggaagccacg gttttcattt acgcgacgtt
     1561 ctgcatccgc gctgcaacca cgcagtagca gctccagcgg tttgcccggc gcgatgttca
     1621 tttctgcacg gatgttacgt accgcaacga tcgcctgctt cagccactcg gtgtcggcca
     1681 gtgcggcttc atcaacctga gatgcatcgt actgcgggaa cggctgcagc atgatggtgt
     1741 cggcagtgat accgcaaagt actttcacac gctgccagat ggtttcggtg atgaacggaa
     1801 tgatcggatg cgcgaggcgc agcagacctt ccagtacagt caccagcgta tggcgagtac
     1861 cgcgcagttc tgcttcggtg ccaccgttca ttaccggctt ggtcagctcg agataccagt
     1921 cacagaactg gttccaggtg aactcataca gaatgcctgc ggcgatatcg aagcggaagc
     1981 tgtccagcgc ttcgcggtac gctttgatgg tctggttgaa ctccgccaga atccagcggt
     2041 ccgccagcga cagcgtcatt tcgccgccgt tgaagccgca atcctgacct tctgtgttca
     2101 tcagcacaaa gcggctggcg ttccacagct tgttacagaa gttacggtaa ccttccaggc
     2161 gcttcatatc ccagttgatg tcacgaccgg tagacgccag tgccgccagg gtgaagcgca
     2221 gcgcgtcagt accgtgcggt tcaataccgt tcgggaactg cttctcggtg cgcttacgga
     2281 ttttgtccgc cagctgtggc tgcatcatat tgccggtgcg tttttccagc agttctggca
     2341 gcgaaatacc gtcaaccata tccagtgggt caataacgtt acccttggat ttggacatct
     2401 tctggccttc gtcgtcacga atcaggccgg tcatgtaaac ggtgtggaac ggcacctgcg
     2461 gtttgccatt ttcatctttg atgaagtgca tggtcatcat gatcatgcgg gcaatccaga
     2521 agaagatgat gtcgaagccg gataccatca cgctggttgg gtggaactga cgcagggcgt
     2581 cggtgttttc cggccagcct agggtagaga aggtccacag tgcagaagag aaccaggtgt
     2641 cgagaacgtc ttcgtcctga cgcagggcaa cgtcggcacc gaggttattt tctttacgca
     2701 cttcgtcttc gttgcggcca acataaacgt tacccgcttc gtcataccat gccgggatac
     2761 ggtgacccca ccacaactga cgagagatac accagtcctg aatatcgcgc atccaggaga
     2821 agtacatgtt ttcgtactgc ttcggtacga actgaatgtc gccgttctca accgcttcaa
     2881 ctgccggttt cgccaggaca tcggcacgca cgtaccactg gtcggtcagc attggttcga
     2941 tgaccacgcc gccacggtcg ccgtaaggaa cggtcaggtc atgtggttta atttcttcca
     3001 gcaggccgag tgcgtcaacc gcagcaacga ccgctttacg tgcagcaaaa cgctccagtt
     3061 tctggaattc tgccgggatc tcgctggaat aaacgtcaga ttcgttacct ttggtatcga
     3121 acacctgggc actttcacgg atatcgccgt caaaggtcag gatgttgatc atcggcaggg
     3181 cgtgacgttt accgacttca tagtcgttaa agtcgtgcgc cggggtgatc ttcacgcagc
     3241 cggtgccttt ttccatgtcg gcgtgttcgt cgccaacgat cggaatacga cggttaacca
     3301 gcggcagaat gacatatttg ccaatcagat ctttgtaacg cggatcttcc gggttaacgg
     3361 ctacgccagt atcgcccagc agggtttccg gacgggtagt cgcaaccacc agataatctt
     3421 taccgtctgc ggttttcgcg ccgtcagcca gcggatagcg gatgtgccac atcgaacctt
     3481 tcgattcgcg gttttccact tccaggtcag agatagcggt gcgcagtttc gggtcccagt
     3541 ttaccaggcg cttgccacgg taaatcaggt cttctttata cagacgaacg aaaacttctt
     3601 tcaccgcatt ggacaggcct tcgtccatgg tgaagcgttc acgctcccag tccacggagt
     3661 tgccgagacg gcgcatctga cgggtaatgg tgccgccgga ttccgctttc cattcccaga
     3721 ttttgtcgat gaaagcttcg cggccgtagt cgtgacgggt tttaccttct tctgcggcaa
     3781 tcttgcgctc aacgaccatc tgggtagcga tcccggcgtg gtcagtaccg acctgccaca
     3841 gggtgttttt gccctgcatg cgctgatagc ggatcatggt atccatgatg gtttgctgga
     3901 aggcgtgacc catatgcaaa ctgccggtga cgttcggcgg cgggatcatg atgcagaaac
     3961 tttcctggct ttcatcgcca ttaggcttaa agtagccctg cttttcccag tgctcgtaaa
     4021 gcggctgttc gatatcttgt gggttatatg tcttttccat tatttccagg ttgccgtatt
     4081 caggttgaaa ccagccacgc ggtaggcttt atagcgttcg cgcgccagtt gtttcagaga
     4141 atcttcataa ggaacgaagt ctaccacttc tgtgaaagcg gtggcaaaat ctgcaaagct
     4201 tgttcgcaga ctaatcaata tatcgcgcgg gctgctgctg cgtttttgcg gccaggcgat
     4261 ctccaccggc gcaccgccgc gcggtccttc tcccgctaaa ttatgcggaa caaagctttc
     4321 tgccggacgc gcccacaggg cttcatccag ccggtaagcc tgcttttcat cttcacaggc
     4381 gatgagcacg cgcttaccac tgcgccaacg ttctgcggca atttcacaca ccagctgctc
     4441 aacggcgctt aagccatcga cggtggtgtc attgtccaga aggtagaacg tcgcgttttt
     4501 cataaatggg gcttcttgtg gtggatttaa atattgctgc attcctcacc ctaaaagatg
     4561 agcaatgaat ttctattgcg ctcgatcggt tccctctccc ttagggagag ggttagggtg
     4621 agggtaaaac gcaaacttac tcttcgccgt taaacccggc gcggtttaac aggaactgtg
     4681 ccagcaacgc taccggacga ccggttgcgc ctttcgcttt accagaacgc caggcggtac
     4741 cggcgatatc caggtgcgcc cagttgtact tacgggtaaa gcgtgacagg aagcaacctg
     4801 cggtaatcgc cccaccagga cgaccgccaa tgttcgccat atcggcaaaa ttggactcca
     4861 gttgttcctg atactcgtca cccagcggta agcgccatgc gcggtcaccg gattgttcag
     4921 acgcggcaat cagttcatgg gccagcggat tgtggttcgc catcagacca gtaatgtgat
     4981 gacccagcgc gatcacgcag gcaccggtca gcgtcgccac gtcaatcacc gcttccggct
     5041 caaaacgctc aacgtaagtt aacacgtcgc acagtaccag gcggccttca gcgtcggtgt
     5101 tgagcacttc aacggtttga ccagacatgg tggttaacac atcgcccgga cgataggcgc
     5161 gaccgcctgg catgttttca caacctgcca gcacgccgat aacattaatc ggcagttgta
     5221 gctccgcgac catccgcatc acgccgtaaa ccgccgccgc gccgcacata tcgtacttca
     5281 tctcatccat gccttctgaa ggcttgatgg agataccgcc ggagtcgaag gttaaacctt
     5341 tacccaccag cacgattggg cgcgcatctt ccgacgcgtt gcctttgtac tcaatcaccg
     5401 acatcagtga ttcgttttgc gaaccctgac caaccgccag ataggaatgc atccccagct
     5461 ctttcatctg ctgttcgccg ataacgcggg tgatgacatt cttgctgtag ctgtcagcca
     5521 gctggcgcgc ttgtgaagcg aggtaagcgg cgttacagat attcggcggc atattgccga
     5581 gatcttttgc tgctttaatc ccggcggcaa tcgccagacc gtgctggatc gcgcgctcac
     5641 cgctggtcag ttcacggcgg gtcggcacgt tgaacaccat tttacgcagc ggacgacgcg
     5701 gttcgctctt gttcgttttc agctgatcga aactgtagag cgtctctttt gctgtctcga
     5761 cagcctgacg cactttccag tagttgttac ggcctttaac gtgcagttca gtcagaaagc
     5821 agaccgcttc cattgagcca gtatcattca gcgtattaat ggttttctga ataacctgct
     5881 tgtactgacg ctcatccagc tcacgttctt tgccgcaacc aataaggaga attcgctcgg
     5941 aaagtacatt cggaacatgg tgcagcaaca atgtctgccc cggttttcct tccagttcgc
     6001 cccgacgtag cagggcgctg atgtacccat cgctgatttt atcgagctgt tctgcaatcg
     6061 gagaaaggcg acgtggttcg aagacgccca cgacgatgca ggcactccgc tgtttctccg
     6121 ggctaccgct ttttacacta aactccatgc actacgctcc tgaatcttaa agacaacggc
     6181 ggtggctaca gatagaattg caagctttcg taactcatgt ccgctgttgc gatgacttcg
     6241 tgttaatctt aacgttatta cggcattggc acgtcagaac aaaatctgag aggtgaatcc
     6301 gttgagtata attatcttag cgacgatttc gacgactcaa gagaataaat gacgtttaag
     6361 ccatgaaaca agctaaaatc ctgcaaaaga cgagttttta cgggcgtatt taaagtgata
     6421 atcataagat atctggtgcg ggagacgctc aaaagccagc tggcgatact cttcatcttg
     6481 cttttgatct tcttctgtca aaagttagtg aggatcctcg gcgcagcggt tgacggcgat
     6541 attccggcga atctggtgct ctcccttctc gggttgggcg tgccggaaat ggcgcagctt
     6601 atcctgccat taagcctgtt cctcgggctg ctgatgacgc tgggcaaact gtataccgaa
     6661 agtgaaatta cggtaatgca tgcctgcggc ctgagcaaag cggttctggt gaaagcggca
     6721 atgatccttg cggtattcac ggcaatcgtc gcggcggtta acgtgatgtg ggcgggaccg
     6781 tggtcatcgc gtcatcagga tgaagtgtta gcagaagcga aagcgaaccc tggcatggcg
     6841 gcgctggcgc aagggcaatt ccagcaagcg actaatggca gttcggtgct gttcatcgaa
     6901 agcgttgacg gcagcgattt caaagatgtg ttcctcgcgc aaattcgacc aaaaggtaat
     6961 gcacgtcctt ctgtggtggt ggccgattcc ggacatttaa cccagctgcg cgacggctcc
     7021 caggtcgtca ctctcaacca gggaacgcgc ttcgaaggca ctgcactgtt acgtgatttc
     7081 cgcattacgg atttccagga ttatcaggcg atcattggtc accaggcggt ggcgctcgac
     7141 ccgaacgata ccgaccagat ggacatgcgc acattgtgga acactgacac cgatcgtgct
     7201 cgcgcagaac tgaactggcg tatcacgttg gtgttcaccg tgtttatgat ggcacttatg
     7261 gtcgtaccgc tgagcgtggt taacccgcgt caggggcgcg tactatcgat gctgccagcc
     7321 atgctgctgt atctgttgtt cttcctgatc cagacctccc tgaaatcgaa cggcggtaaa
     7381 ggtaagctgg acccgacgct gtggatgtgg accgttaacc tgatttatct ggctttggcg
     7441 attgttctca acctttggga caccgtgccg gtccgccgcc tgcgcgccag tttttcgcgt
     7501 aaaggagcgg tgtgatgcaa ccttttggcg tacttgaccg ctatatcggt aaaactattt
     7561 tcaccaccat catgatgacg ctgttcatgc tggtgtcgct gtcgggcatt atcaagtttg
     7621 tcgatcagct gaaaaaagcc gggcagggga gttacgacgc gttaggcgca ggaatgtata
     7681 ccttgctgag cgtgccgaaa gatgtgcaaa tcttcttccc gatggcagct ctgcttgggg
     7741 cgttgcttgg tcttgggatg ctggcgcagc gcagcgaact ggtggtgatg caggcttctg
     7801 gtttcacccg tatgcaggtt gcgctgtcgg tgatgaaaac cgccattccg ctagtcttgc
     7861 tgacgatggc gattggtgaa tgggtcgcgc cgcagggcga gcagatggcg cgtaactacc
     7921 gtgcgcaggc gatgtacggc ggctcgttgc tctctaccca gcaaggctta tgggcgaaag
     7981 atggcaacaa cttcgtctac attgagcggg ttaaaggtga cgaagagtta ggtggcatca
     8041 gcatttatgc ctttaacgaa aatcgtcgtc tgcaatccgt acgctatgcc gctactgcga
     8101 agtttgaccc ggaacataaa gtctggcgtc tgtcgcaggt tgatgaatct gatctgacca
     8161 atccgaaaca gatcaccggt tcgcagacgg tgagcggcac ctggaaaacc aacctcacgc
     8221 cagacaaact gggcgtggtg gcgctggacc cggatgcact ctcaatcagc ggtttgcaca
     8281 actacgtgaa gtatctgaag tcgagcggtc aggatgccgg acgttatcag ctcaatatgt
     8341 ggagcaaaat cttccagccg ctatccgtgg cggtgatgat gctgatggcg ctgtcgttca
     8401 tctttggccc actgcgtagc gtaccgatgg gcgtgcgtgt ggtcaccggt atcagcttcg
     8461 gttttgtctt ctacgtactg gaccagatct tcggcccgct gacgttggtt tacggcatcc
     8521 cgccgattat cggcgcactg ttgccaagcg ccagcttctt cttaatcagc ctgtggctgt
     8581 taatgagaaa atcgtaacca agcaagggag cgaaagctcc ctttcacatt gttgataaag
     8641 tcaaatacga gttcgatcgg tcccctcgcc cctttgggga gagggttagg gtgaggggga
     8701 cagatcgcgc ctgacttacc ttcttctccc ccccagcaaa ctccccaaca tcccgcgtac
     8761 aatctgattc gtcacctggc gagcggcgct tttggccatt gtctgcacca caccatcttt
     8821 cttcccgccg cgtggtccgg tagtgccgaa caaaatatcc ttcaatccac caagaatgcc
     8881 gtcatctacc gctatctctt tccctttcgc tggaggatta ttttgctgtt caatactcgc
     8941 ctgtacgcct ttttgcaaca tctcataggc ggattcccgg tccacctcat cctcgtactt
     9001 gccatacacc ggagagtgat taatcaagcc attacgctca tcttccgtca ccggtcccat
     9061 ccgcgaacaa ggcgcaatca ccatcgcccg ttccaccact gaaggacttc ctttcacatc
     9121 aagaaacgag attaacgcct cgccggtccc cagttcctgg attgcctttt cggtatcaaa
     9181 tgccggattg gcccgcatgg tttgcgccgc agcttttacc gctttctgat ctttgggcgt
     9241 aaaagcacgc aaagcgtgtt gaacgcgatt accgagctgc ccgagcacat tatccggaat
     9301 atcagacggg ttttgcgaaa cgaaccagac gcctacgcct tttgagcgaa taagccgtat
     9361 cacctgctca atcttatcca gcagtacctg cggtgcatcg ttaaacagca gatgtgcctc
     9421 gtcgaagaaa aacaccagtt ttggtttctc cagatcgccc gcttccggca attgttcata
     9481 caactctgaa agcatccaca gcaggctggc ggcgtacagt ttcggcatct gataaagctt
     9541 ctcggcgctg aggatattga taacgccttt accgttggta tcggtgcgca tccagtcttt
     9601 gatatccagc atcggctcgc caaagaagtg tgctgcgcct tgctgttcca gcgacaacag
     9661 tccgcgctgg atggcaccaa ccgatgcgct actgatatta ccgtactgat tctggaaaga
     9721 tttggcgtta tcgccgatgt actgggtaat cgcccgcaga tctttaaagt cgagcagcaa
     9781 caatccctga tcgtcagcaa tacggaagat gatattcagc acgccagatt gcacatcgtt
     9841 gagattcaac agccgcgcca gcaacagcgg ccccagatcc gaaaccgtcg cccgcaccgg
     9901 atggcctttc tcgccaaaga tatcccacac caccaccgga ttagcatgcg gttgccagtc
     9961 attgacgccg atatttttaa gccttgcgag tagtttttcc gatgccgttc ctgcctgcgc
    10021 gatgccggtc agatcgcctt tcacatcggc cataaacacc ggaacgccga tttccgacaa
    10081 tgactctgcc agtttttgca gcgtaacggt tttacccgtc cccgttgcgc cagtaatcag
    10141 cccgtgacgg ttagccattc ccggcagtaa aaacagttct gtgtccggcg tgcgggcaat
    10201 taacaggggt tcgctcatga gatatcctcc agtttcctgc ctggagtata ggcaacgtcc
    10261 ggcaaagaga atacgtgtaa tgaagagaaa ataacctaaa gcgtgttgcc gtgataaatc
    10321 tgatacccga ggtcaacagt gttgtggttg tgatcgttat ttttaatttt gctcagcaac
    10381 atttgggcgg ccatccgacc aatatcaaaa cggggagtaa ttacgctggc gaggcttggg
    10441 atcatttgcc tgccaatctc cagcccatga aaacccgcaa tggaaatttg ttctggcacg
    10501 gcgaggtttc gctcgcggca cagcagaagc gcacccatcg caatatcgtc attggtgcaa
    10561 aatacaccgt ctaaatccgg gtttgcgcta agggcatcac gcattaactg cattcctaaa
    10621 tgaatggatg agatggcgcg tggattcatg cgtaatgggg aaagattatg cagcatcatc
    10681 gcatcgcaat acccctgata acgctgttca tcgcgggtgt catctttgga accgagatac
    10741 agaattttgt gcctgactcg cttctccagc atggtacaca ccatgtcaaa agcggcctgc
    10801 cgattatcaa aaccgacctc catatccagc cgttctccct gtacatccat caattccacg
    10861 accggaatgg tggctgaacg cagaaatttc accgtcctga tggtgtgata tttttccgaa
    10921 agtataatcc cgtcaatgtt ataagagagc aggttaatca ccgactcttc ttcggaatcg
    10981 cgatcgtaat tgtaattagc aataagggtc tgataattat gctcagaggt gacggattct
    11041 attcccgcga gaatgtcagc gaagagttga ttttgaaatg acggaatcaa gataccgagg
    11101 gtataacttt gtgcattcaa caacatgccg ggagcgcgat tagggatata attgatctct
    11161 tccataattt tggcgatgcg ctcgcctgtt tcctttgcca cctttttcgg cgagcggata
    11221 taacgactca cggtcatttt tgttacgcca gccagcgtag cgatatcctg taaagaaatt
    11281 ctgtgattgc gcataacttc tcccaacgtc gggggaatct tcaatcttcc cccgaaacta
    11341 catcacaata ttttattctt tcagtgcaat acggcgttaa tggctaacac gcccagcaaa
    11401 cccataatag aaataagggt ttccatcacc gtccaggtac gcaacgtttc accaaccgtc
    11461 agattaaaat accctttaaa tagccagaag ccagggtcgt ttacgtgtga cgcgatcacg
    11521 ctgcccgcac cggttgccag taccattaaa gcgggatcgg catgggtaac gttgataatc
    11581 ggcaacacca cacccgcggt ggtaatggcc gcgacggtgg cagagcccag agcgatacgt
    11641 aacagcgccg caacagtcca gcacatcaat aacggcgaaa gcgtagttcc ggtcattaag
    11701 tgtgaaatat agtgcccgac accgctatct actaatacct gcttaaacgc gccgccgcca
    11761 gcgataataa acacaatcat cgcgatagcg cctatagaat ccccaatgat atccatgatt
    11821 tgctcgatgg tgcgtccatt acgtcggccc agtgtgaaaa tcgcaataac aatggcaata
    11881 aacagcgcaa cggcagggtt accgacaaat tcaaagaaga ggcgcacggt gttagttttc
    11941 ggtaacgtaa tttcacaaac ggcggcgata gccatcagga tgaccgggat cacggcagcg
    12001 aaaatactgt tccagaagga gggcatctcc tcttcgctaa acagatgagg attaaataag
    12061 ccttccggtg gtgctttctc aaagcgagtt agcagtttag aaaacagcgg tcctgcgaca
    12121 ataactgtcg gaatggtaat gataaatcca tacagtaaag tcgttccgag attagcctca
    12181 aagatagtcg cgatggcagt aggccctgga tgtggcggca gaaaacagtg ggttacagag
    12241 agcgctgcta ccattggtac gccaacatac aacaggggta atcctgatga tgctacgatg
    12301 gtaaatacca acggcaacag caggacaaaa cccacttcaa aaaacatggc gaggcccaca
    12361 accagaccgg tgatcactag cgcccattgc acgcgttttt taccaaaagt agcaatcagc
    12421 gtagtggcga tacgttgtgc cgcacccgta tcagaaatca gcttgcctaa catggccccg
    12481 aaaccgagga tcattgccag cccgccgagc gtgctgccga taccattttg tatagagtgc
    12541 aggacggcct gtgcatccat cccttcggca aatccgacga cggcagctac cagaacgagg
    12601 gcaataaagc cgttaacttt aaagccgaac atcaggataa gaagcagcgc gacgcctgcc
    12661 gcaataatga ttaatggcat agtctttatc tcttattagt gccagaaaag cagcgctttc
    12721 gcgctgcacg tagggtagta ggtgttaaac agccactaac atgccgccat caacaaacaa
    12781 caggtggccg tttacgaaat cagaggcttt tgaagaaagg aacaccgcag caccaatcag
    12841 ttcctgcgga tctccccagc gtgcggcggg ggtccgtttg cacaaccagg cggtgaaggc
    12901 ttcgtcctca accagtgctt tagtcatttc tgttttgaaa tagcccggcg caataccgtt
    12961 gacctgaata ttgtggcgcg ccagctcgac gcacatgccg cgggtgagca tttttaccgc
    13021 ccctttcgat gcggcataag gggtgatggt gtcacgtccc agttcgcttt gcatcgagca
    13081 aatattaata actttacctg ccttgcgttc aaccatgtga cgagttaccg cttgcgatac
    13141 caggaacacg gcggtctggt ttactgcgat cacatcattc cactcttgtt cagggaactc
    13201 agtaaaagga tgacggcgct ggataccggc gttattcacc agcacatcaa tggggccgat
    13261 gtccttttcg atatgttcaa cggcggcatc aatttcatgt ttatgagtaa cattaaaagg
    13321 tgcggcaacg gcctgaatac cctcctggtg gagtttttct acagcaagtt ctgcgcgttc
    13381 ggcagtaata tcattaataa ttatttgtgc gccatattta cccaggccgg ttgccagtaa
    13441 aaagccaatg ccctgtgctg aaccggtaat caagatattt tttcctgcca gtgaaaatag
    13501 atcgttcatt gtattattcc tttatttatc tcttagaaaa caagctggac ttttgctgcc
    13561 tgggttttat caccggcgaa acgtagcgcc tcttccaggt cagtgaaggg atattcagca
    13621 ctcagtaaag gcagtggatt gataacgcca ttcgccagcc atgacactgc ggtattaaat
    13681 tcgctggtaa aacggaaaga gcctctgagt gaaatctcct taccaatcaa cgtcatcatt
    13741 gggaattctg ccatcgcgcc tcccatacct acctgcacca ttacgccgcg tgcacgagtg
    13801 acctccagac aggtattcac tgatgaagga tgaccggaca cttcaaagct gacatcgaaa
    13861 tagccttttt ccgctttcca gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc
    13921 gcccccatct ctttgcccag cgaaagggaa cggggactca catcagcaca gacaatttcc
    13981 gcggccccca gtgttttcac tgcactgaca atcaggcagc caatgggtcc aacaccggaa
    14041 ataaatactc gcttgccctg taactcgccg gcctgatgtg cggcatgaat cgcgacggct
    14101 aaaggttcgg caaaagccat aaccttttcg tcagctttgg ccggataagg gacacattgc
    14161 gacgtttcga ccattttata acgggtaaaa ccaccatcaa catgagggaa atacatggca
    14221 ctgccaaaaa aacgcatctc tgtacactga ttctcgttat gttcaatgca gtatttgcag
    14281 tgaccgcacg gtttagacgg attaatggct accgtttgcc cttcatgtaa ttctgatgag
    14341 tcgctatgaa taactttacc gataacttca tgacctaaca ccatcggtgc ctttatcatg
    14401 aaattaccta cttttccttc ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg
    14461 gttatttgta ctaatgttcc attattattc caatctatcg tctgctcggt aacggcaaca
    14521 gttttcttgc ccgcaacaac gcaggactgt gttttcactt gcataaattc ctcactggtc
    14581 aggtagacac ctcggaagca tttaagcggt tttaattgtc attatttgtg atgaagatca
    14641 cgtcagaaat tgttacatta ctatgttacg cataacgtga tgtgccttgt aattcttatc
    14701 agcagaaata aaaaaacgtg aaattattat gccaccaggc gtagtatcgc agcaggtaag
    14761 atgattcagg agattttaaa tggcgggtga aagctttatt ttgatgggcg tttcagggag
    14821 tggtaaaaca ttaattggta gcaaggttgc cgcgttatta tctgctaaat ttattgatgg
    14881 tgacgatctt catccagcca aaaatataga taaaatgtcg cagggtattc cattatctga
    14941 tgaagatcga cttccctggc tggaacgctt aaatgatgct tcatacagtc tttataaaaa
    15001 gaatgaaaca ggatttattg tctgttcatc attaaaaaaa cattatcgtg atattttacg
    15061 caagggtagc ccccatgttc atttcctctg gttagatggc gactatgaaa ctatcctcgc
    15121 gcgaatgcag cgtcgggctg ggcattttat gccggtagcg ttactaaaaa gtcagtttga
    15181 ggcgctggag cgtccacaag cagatgaaca ggatattgtg cgcattgata tcaaccatga
    15241 tattgccaat gtcaccgaac aatgtcggca ggctgtgctg gcgatacgac aaaacagaat
    15301 atgtgcgaaa gagggcagcg cctcagatca gcgctgcgaa tgattttcaa aaatcggctt
    15361 tcaacaccac gcggtaacgc gccttaccgt cgcgcacatg ctggatggcg tcgttaattt
    15421 tcgacatcgg gaacagttcg gttgtcggcg caaccttgct gcgggcggca aagcgcatca
    15481 gcttacgcag ttcataaggc gtgccggtag cagagccaga gacgctgcga tcgcccgcaa
    15541 ttaacgtaaa ggccggaaca gacagcggcg tgagaaccgc accgacagtg tggaaattac
    15601 cgccgtacgt cagcgcctca aaataaggct gccagtcgag gctgacgttc acggtattga
    15661 taatgagatc aaactgcccc gccagtgctt tcagtgcctg cggatcgcgg ctattcacca
    15721 ctttatcggc acccatcgcc agcaattcct gctctttcgc cggattagaa ctaaaggccg
    15781 tcacctcaca tcccattgcg tgcagaagtt ttatagcgat atgccccagc ccgccaatac
    15841 caattacccc aacgcggctg gtagcagtga tatggtgcat caacagtggt ttaaagaccg
    15901 tgataccgcc gcacaacagc ggcccggcag attcaatgtc gatattttcc ggcagtggaa
    15961 tcacccattg ccagtctaca cgcaacttct cggcaaaacc tccgcgattc ataattgttg
    16021 gcaccgcacc ttgctcacag ttgatctgat ttccgctaat acaggcgtcg cagtgaccac
    16081 agctacgcgc cgtccagcca atcccgacat gctgaccgac ctgcaaacct ttatcctgcg
    16141 cggcactccc gagcgccacc acgcgaccaa tcacctcatg cccggcaacc agcggatatt
    16201 gtgaaaatcc ccattcgtta tcgatcatcg acagatcgga atggcagatc ccgcagtaat
    16261 ccacctgcac ttcaacatct tgtggcttca gctcaccggg atcgtactca taaacttcca
    16321 gttcgccgcc cgcttctttt gcggcatagc tttttatcat tgacattttt tggtccttct
    16381 ctggtgtggt ctgggaagtg tagagcatgg cagggcgggg tgcatggagt gtgacaaagg
    16441 ttacacatcg ctgtacgcaa tgctgaaaat ttcagcactt agcgagatgc gagcaagctg
    16501 gcgcttgcat ggtggcgtgc gacaggtata atccacaacg ttttccgcat acctcttcag
    16561 tgccgaagtg gcgaaatcgg tagacgcagt tgattcaaaa tcaaccgtag aaatacgtgc
    16621 cggttcgagt ccggccttcg gcaccaaaag tatgtaaata gacctcaatt gaggtctttt
    16681 tttatgcctg aaatccagta tttatctatc tttcccgcta tattaactcc ctcaaggtca
    16741 atcgatatca acgtacatct accaacacat gttggtacaa atgatggtat ttccggttcg
    16801 ataatgcttg taccaacagg gagggaatac gcatggcatt aacagatatc aaagtcagaa
    16861 cagccaaacc aacggataag caatataagc tgactgatgg tggaggtatg catctgcttg
    16921 tccatccaaa tggttctaaa tactggcgtt tgcagtaccg ttatgaggga aagcaaaaga
    16981 tgctggcact tgggatttat cctgaaatca cactagcgga tgccagagta cgtcgcgacg
    17041 aagcacgtaa gctgcttgcg aatggcgtcg atccgggaga caaaaagaaa aatgataagg
    17101 ttgaacagag taaagcacga acctttaaag aagtcgcgat cgagtggcat ggctccaata
    17161 aaaagtggtc tgaagatcat gcccatcgtg tactaaaaag tctggaagat aatctttttg
    17221 cagcgcttgg tgaacgtaat atcgctgagt taaaaactcg agatttatta gcacccatta
    17281 aggccgtgga gatctctgga cgtcttgaag tggctgctcg tcttcaacag cgcactacag
    17341 ccatcatgcg ctatgcagtg caaagtgggt taattgatta taacccggca caagagatgg
    17401 ctggggcggt tgcttcctat aatcgacagc atcgtccggc gcttgaatta aagcgtctcc
    17461 ctgagttgct tacaaaaata gatagctata ctggtagacc gttaacccga tgggcgacag
    17521 aactcacttt gctgatcttt attcggtcca gtgagctgcg ttttgctcgt tggtcagaga
    17581 tcgatttcga agcgtctata tggactatcc caccggagcg ggagcctatc cctggagtga
    17641 agcattccca tagaggctca aaaatgcgta caacgcatct agtgcctctt tcaacgcaag
    17701 cccttgcaat tttaaagcag ataaaacagt tttgtggggc tcatgacttg atatttattg
    17761 gtgatcacga ttcgcacaag cccatgagtg agaatacagt aaatagtgcg ttacgggtca
    17821 tggggtatga tacaaaagtt gaggtctgtg gtcatggctt ccgaacaatg gcctgtagtt
    17881 cattaatcga atcaggtttg tggtctcgtg atgctgttga acgtcagatg agccacatgg
    17941 agcgaaattc agtgagggct gcgtatatcc ataaagcaga acatctggaa gaacggcgat
    18001 tgatgctaca gtggtgggca gattatctgg atgcgaatag agagaaaggt atctgcccgt
    18061 ttgactatgc aaaaattaac aatccattaa aa
//
LOCUS       DAZFAH010000087        18047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFAH010000087 DAZFAH010000000
VERSION     DAZFAH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.087"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(8..766)
                     /gene="aroD"
                     /locus_tag="ACHJWB_002739"
     CDS             complement(8..766)
                     /gene="aroD"
                     /locus_tag="ACHJWB_002739"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4314346.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(797..1663)
                     /gene="ydiB"
                     /locus_tag="ACHJWB_002740"
     CDS             complement(797..1663)
                     /gene="ydiB"
                     /locus_tag="ACHJWB_002740"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4314347.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVY
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1675..2940)
                     /locus_tag="ACHJWB_002741"
     CDS             complement(1675..2940)
                     /locus_tag="ACHJWB_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4314348.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPILVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3167..4380)
                     /locus_tag="ACHJWB_002742"
                     /pseudo
     CDS             complement(3167..4380)
                     /locus_tag="ACHJWB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4480..4836)
                     /locus_tag="ACHJWB_002743"
     CDS             complement(4480..4836)
                     /locus_tag="ACHJWB_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4314349.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4972..5079)
                     /gene="rprA"
                     /locus_tag="ACHJWB_002744"
     ncRNA           complement(4972..5079)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJWB_002744"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5265..6377)
                     /gene="ydiK"
                     /locus_tag="ACHJWB_002745"
     CDS             complement(5265..6377)
                     /gene="ydiK"
                     /locus_tag="ACHJWB_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4314350.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6766..9822
                     /locus_tag="ACHJWB_002746"
     CDS             6766..9822
                     /locus_tag="ACHJWB_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4314351.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9819..10229
                     /gene="menI"
                     /locus_tag="ACHJWB_002747"
     CDS             9819..10229
                     /gene="menI"
                     /locus_tag="ACHJWB_002747"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4314352.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10329..10517
                     /locus_tag="ACHJWB_002748"
     CDS             10329..10517
                     /locus_tag="ACHJWB_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4314353.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11065..11433
                     /gene="sufA"
                     /locus_tag="ACHJWB_002749"
     CDS             11065..11433
                     /gene="sufA"
                     /locus_tag="ACHJWB_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4314354.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11442..12929
                     /gene="sufB"
                     /locus_tag="ACHJWB_002750"
     CDS             11442..12929
                     /gene="sufB"
                     /locus_tag="ACHJWB_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4314355.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12939..13685
                     /gene="sufC"
                     /locus_tag="ACHJWB_002751"
     CDS             12939..13685
                     /gene="sufC"
                     /locus_tag="ACHJWB_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4314356.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13660..14931
                     /gene="sufD"
                     /locus_tag="ACHJWB_002752"
     CDS             13660..14931
                     /gene="sufD"
                     /locus_tag="ACHJWB_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4314357.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14928..16148
                     /gene="sufS"
                     /locus_tag="ACHJWB_002753"
     CDS             14928..16148
                     /gene="sufS"
                     /locus_tag="ACHJWB_002753"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4314358.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16161..16577
                     /gene="sufE"
                     /locus_tag="ACHJWB_002754"
     CDS             16161..16577
                     /gene="sufE"
                     /locus_tag="ACHJWB_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4314359.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16726..17730
                     /gene="ldtE"
                     /locus_tag="ACHJWB_002755"
     CDS             16726..17730
                     /gene="ldtE"
                     /locus_tag="ACHJWB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4314360.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPTGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17794..18030)
                     /gene="lpp"
                     /locus_tag="ACHJWB_002756"
     CDS             complement(17794..18030)
                     /gene="lpp"
                     /locus_tag="ACHJWB_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4314361.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4720 a         4386 c         4654 g         4287 t
ORIGIN      
        1 ttattgctta tgcctggtgt aaaatagtta ataccgtgcg taaatcattt accgagattt
       61 gccctggtgc agaggctttt tttaccgcac caaaagttgc cgccgagcca aatacttcac
      121 cagccagacg agaaattacg ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg
      181 catactgctc ctgcatctcc agggtcgcgg taagcaacgt cagcacatcg ctggtacttt
      241 gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg
      301 caatgatttc ttcggcttcc ggcgttttat ggaagtcatg gttggacatg acaactttca
      361 catcatgcgc gtgggcgtag gcgacggttt ctttaacctg atcatcgccg gtaaataact
      421 ccagatcgat catatcaacc aggccgctgt cgatggctgc acgattgaga gcaatataag
      481 cctcggtgga aatcgcctgc tcgccgcctt ctttggcact gcggaaggta aacagcagcg
      541 gtttttctgg catggtttca cggagaattt ttgccgccgc catgacagac tccacattgg
      601 agaggtcggc aaagtggtcc acacgccatt ccagaatatc aaagtccgct tcacgatagg
      661 cgagagcttc ggatttcacg ctggcgatat ctttcgccat cagcgagacg atgattttag
      721 gtgcgcccgt accaatgacg agatctttta cagttacggt tttcattttt taccctttct
      781 gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg tttaacatat tccagaggga
      841 aatctttgcc agtccataac gtgaactgtt cagccccttg ccacaacaac atgccgtatc
      901 catcaatcgt tttgcaacca gcttgttgtg cctgctgcaa taacttcgtc atatgcgggt
      961 tatacacgca ttcagtgacc agcagtcccg gatgtaacag actgatatca taaaccaatg
     1021 attcattctc aaggggtttc atacccactt ttgtgccatt ggttaaaatg tcggcggaag
     1081 ccagggcttc agcaaaggct tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg
     1141 tgttttcgtt aacccgctgc gcgaaggcga gggctttatc gaagaactca tcccgacggt
     1201 taaagagttt aatttctttt aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg
     1261 cacctccggc ccctaacagc accattgttt tgcctttgat atcaaaaccg ctctctttaa
     1321 tggcgcgaat atggcccgtg ccgtcggtgt tatagccgcg caggtagcca tcatcattaa
     1381 cgatggtgtt gatggcaccc accagtttgg ctgctggcgt taattcatca acatattcac
     1441 acgccagttg tttgttcggc attgatatac cagttccgcg cattttgagg gcttttaatc
     1501 cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa ggccatatag gtaaatggca
     1561 atcccgcttt ttctaaggct ttattctgca tttcaggcga taaactgtgg cggataggat
     1621 aggccatcag cccaatcaat tcgtattttg cggtaacatc cataattaac tcctttaacc
     1681 tctatgctta attgtgttta accgggtgct aaatttgcgc tcgccaaacc gcacatcatt
     1741 ctcaggaata ataaaaacgc ggtaatagcg gataaaaaca ataattgcgg taatcagggc
     1801 cagcagcgcg aaagtaaaat cgagaacaat gatatattgc aggccgatgt tcgacagata
     1861 accggtaatc agcggaataa caaagttagc cagtccaccc atcatcatat aaatactggt
     1921 gactttggct ttgctttttg ggaaaaactc tgacatgacc gaaacaccga gctgtaaaat
     1981 gccgccagct gctgagaaac cgataacaaa ggcgccggca ttacacacca gtggagaagg
     2041 gtacaggtag ataatggctg ctgttattgt tgccagtgca gagttaaata cattagccca
     2101 gatgggccgg accatttttt tcagtagtgc ggcaaaaata aagacacaga ccaacgagcc
     2161 catactgtaa taagagatgg tttttaatgc ctcagcttct gacataccag caaaagccat
     2221 cgcatatttg ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa
     2281 cagtaccgat gaaacacctt ccagccagac taacggtttg ctgttcattt gcggtaattc
     2341 attagctacg ctggcgtcca ccaactggct ggggaatttg cttttcaaca gcatcagcgt
     2401 gatcagtaca aacagaatac ccggaataat cagcccatag ccgtaccaga tattattgag
     2461 caacatatag ctcaccagaa ttgggtagaa catttgccca aatgacacca tcgctttaac
     2521 cagtatgacc gccgaaccag aggctttcgg aaagcattcc atgagcgcgg ggtagccacc
     2581 cgtatccagc gctgagttag cgatacctac gcacactgcc agaccgtagg cgagagttaa
     2641 attcgggcaa gcgggaatac caaagaagaa tagcagatac attattactg ccattaatat
     2701 caccgcccga cgaccaaact tatcggagat cacaccgaag aataaaatac tgatcaatcg
     2761 ccccaaacca ataccggaaa ttaagtaggc aatgcccgcg ttgtcagtgg aaaacttttc
     2821 cgccagagat gacatgtttt gggcaagcgt aataacacta atgccgtgca ggaagtagct
     2881 gaagtaaata caaagaacag ccaggataaa tggcgtgctg aaagccttat tttgagacat
     2941 tttcacagct ccttgctgaa agataatttc gttaccgtac gtattgttct tcgacggttt
     3001 acataattac tatactgaag atgattttcc ctgggtacat ggcataaagt ttatgtttcc
     3061 aggaatattg catgtcatag tttgtttttg atcgcgattt tccttagtta gcgcagaaag
     3121 agttatttat ccgtaagatc ttgcatgacc tgaaaagaat attgcattac ccacccggag
     3181 cgacattttc ctttagcgag tgatgccgcg ttttttgcgg ctgcgtaagc cgataaacag
     3241 tgccagtaaa aaaccgatgg cagcgatggc ggtatcgaac cacataatat cggcaatact
     3301 tctttgggac agatgagccg taatcaacgg aatagtaaag gtcgcaatac tgcccgcact
     3361 gtaatagatc cctgtagctt tacctttagc gtaagggaaa cgttcagcca ttaacgtcag
     3421 gccaatttgc acaacacctc cagcagaggt aaaaccaatg acaaaagcaa atattatcac
     3481 cacataaaat gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta
     3541 cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcgg taataaatac
     3601 acaaagcagc gaacccacgg tgtagatact gagtagttta atcgacatag tgtatgacat
     3661 gcctgcaaca aattgtccgt actgtgccag ccactggcta accagataaa acgttgccat
     3721 tgagatatag ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt
     3781 agagctggtg gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca
     3841 acggtataaa aacagagcgt taataaacat aatgcctgca gcaatcataa aggaccaacc
     3901 gaaccacagt tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct
     3961 ggaaacaaat gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat
     4021 caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
     4081 aacataggcg ataatgatgt tattggtgtg caggatgcca aaaaagaagg ccatatagca
     4141 gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
     4201 aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc
     4261 attagtctgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
     4321 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat
     4381 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccattca
     4441 ttattgttgt aacaacgaca attcctttct gtggaaaaat taacaaagcc gctccagatc
     4501 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
     4561 agggtagact cgttgaaatg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
     4621 gcggttattt attttctcta tgatggcatg aagatgtttt tttctttgct gacgcatagc
     4681 caacagttgc tcgactacac gatcaggaat ggcacacttg ccattttccc actgacgcca
     4741 gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
     4801 aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
     4861 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
     4921 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gtggaaaaat gttaaaaaaa
     4981 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
     5041 tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
     5101 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
     5161 gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat
     5221 tagcgggggt taacaatcag gcggcagagc cgcctgatga gaaattactt attcggtttc
     5281 tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg cgggacttct
     5341 tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga
     5401 ccgatcatcc cgaaagcaat caaaccacca ataacaccag agaggatcag gatcagcggt
     5461 aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt gccaaccaca
     5521 ccgctccaca ctaacaatac cgttccccag gtggtatcgc cagtccagta gagccagata
     5581 atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggagag gatcattaac
     5641 accgttagca acgttgcata aggtacgccg gatacggcga ggccgatacc gccaagcact
     5701 gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc
     5761 agcagtacgg cggcatctcc gcgaacgcct gccagacggg ttgcaaaatg gcgaatgcct
     5821 tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat cagcgcacaa
     5881 tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt ggtggtgcca
     5941 atataagggc ggactttcgc catgatcgcc gtccccccca tatccagcaa gttgtgccag
     6001 cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac
     6061 gtcatgtcgc cgctggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta
     6121 accagcaaag caatagggat gataaacacc atcactaata acagcgtcat caccagaacg
     6181 gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg
     6241 ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat ccacagacat
     6301 gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc
     6361 tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac
     6421 gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt taacaggccg
     6481 attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa
     6541 cacgcaaacg atttactcgt cgtatttcaa gttgcatgtg ctgcggctac gttcgttcac
     6601 cccagtcact tactattgta agctcctgga gttcactcgc ttgccgcctt cctgcaactc
     6661 gaaatccata gagtaaataa acatcacctt tcagtgcgct attgcagccg cctgaaagaa
     6721 tagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat tccacagatt
     6781 tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct ggagcaacaa
     6841 ggttttaccg gcgatacggc gacaagttat gccgatcgtc tgacaatgtc gaccgacaac
     6901 agtatttacc aacttctccc cgatgcggtg gtatttccgc gttcaaccgc agatgtggcg
     6961 ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac cccccgcggc
     7021 ggcggcaccg gcactaacgg tcaggcgctc aaccagggga ttattgttga tatgtcccgc
     7081 catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt tgaggccggg
     7141 gtgataaaag atcaactcaa tcagtacctg aaaccgttcg gctacttttt tgcgccggaa
     7201 ctttcgacca gcaaccgggc aacgctcggt gggatgatca ataccgatgc ttctggtcag
     7261 ggatcgctgg tctatggcaa aacgtcggat cacgtacttg gcgtacgtgc ggtgttgttg
     7321 gggggcgata ttctcgatac gcaaccttta cccgtcgaac tggcggaaac gctgggtaaa
     7381 tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg tcagcaacgc
     7441 cagttaatca tcgacaactt ccccaaactt aaccgctttc ttaccggtta cgatctgcgt
     7501 catgtcttta acgatgagat gaccgagttc gacctgacgc gcattctgac gggttcagaa
     7561 gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcccttgcc taaagtgcgc
     7621 cgtctggtga acgtcaaata tgactctttt gactccgcgc tgcgtaacgc gccgtttatg
     7681 gttgaggcgc gggcgctttc ggtagagacg gtggactcaa aagtgctgaa tctggcgcgg
     7741 gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga caaagagatg
     7801 ctcgggctga acattgtgga gtttgctggt gatgatgagg cgctgattga cgagcgagtt
     7861 aatgctctct gtgtgcggct tgatgagctg attgccagcc aacaggcagg tgtgattggc
     7921 tggcaggtgt gccgcgagct ggcgggtgtt gaacgtatct atgcgatgcg taaaaaagcc
     7981 gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc
     8041 tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct gctcgacagc
     8101 cacggcttaa gctacggtat gttcggtcac gtcgacgcag gtgtcttgca cgtccgtcca
     8161 gcgctggata tgtgcgatcc tcaacaagag attttgatga agcaaatctc tgatgacgta
     8221 gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg agcacggcaa aggttttcgc
     8281 gctgaataca gcccggcgtt tttcggtgag gaactttttg cagaactgcg caaagtgaaa
     8341 gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc agaaggtctc
     8401 gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg gcagatcccc
     8461 attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg tttatgcttc
     8521 aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa ccggattcat
     8581 tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc
     8641 gttgatccac tcaaactgga acaagaactg cctgaatccg gcgtcagttt gcggacgtta
     8701 attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt ctcacacgaa
     8761 gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagcgt gttcgaccca gtgccccatc
     8821 aaaattgatg tgccggagtt tcgctctcgt tttctgcagc tctatcacac ccgttattta
     8881 cgcccgctgc gcgaccacct cgtcgctacg gtcgagagct acgcgccgct gatggcacgc
     8941 gcgccgaaaa cctttaactt cttcattaac cagccgctgg tgcgcaaact ctcggaaaaa
     9001 catatcggca tggttgattt gccgctgctg tcggtcccct cgctacaaca acaaatggtg
     9061 gggcatcgct cggcaaacat gacgctggaa cagcttgaat ctctcaatgc agagcagaaa
     9121 gcgcgcacag tgttggtggt gcaggacccc tttaccagct attacgatgc gcaagtggtg
     9181 gcggattttg tccgtctggt cgaaaaatta ggtttccagc ctgtgttact gccattttcg
     9241 ccaaatggca aagcccagca tattaaaggt tttcttaatc gttttgcgaa gacggcgaaa
     9301 aagacggcgg atttcctcaa ccgtatggcg aagctgggta tgccaatggt gggcgtcgat
     9361 ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga ggaacgtggc
     9421 gagtttaacg tcttactggc gaatgaatgg ctggcaagcg cacttgagtc acagccagtg
     9481 gctacagtca gcggtgaatc atggtatttc tttggtcact gtaccgaagt taccgccttg
     9541 ccgggtgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat
     9601 gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa aaaccataaa
     9661 aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact gccgcgtaac
     9721 cgctgtctgg cgaccggata ttcctgccgt agtcaggtca aacgggttga aggcacgggg
     9781 gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa cgaaaaatca
     9841 ccctggaagc actgaatgcc atgggtgaag gaaacatggt ggggttactg gatattcgct
     9901 ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg cggacaaagc
     9961 agcctttcgg gttgctgcat ggaggcgcat ccgtggtgct ggccgaaagt atcggttccg
    10021 ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta
    10081 atcacgtccg ctcggcacga gaagggcggg tgcgcggcat atgtaaaccg ttgcatctcg
    10141 gttcgcgtca ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt ttgtgctgtt
    10201 cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg acgcatattg
    10261 cttttggtca ttatctgcac agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg
    10321 aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt ttacgccgtg
    10381 accagagcaa tctttcacct gcccaatatc tcaaacgtct gaaacaacta gaacttgaat
    10441 ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac tttgcctggc
    10501 gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga cctggattaa
    10561 cctccgtcca ggttcgatga ccttcttaat actgcttatc taccgcctga tattgttcaa
    10621 aaataatact aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag attattctta
    10681 tcgccccttc aagagctaag ccactgagag tgccggagat aagcgccgga tggggttgaa
    10741 acccttaagc ctgtgtcgca cagacttaag ggttttctta tttctgaacg ctgcactttt
    10801 ttacctatct cttaaatagt cattttcaat acaacttttt ctttttcatc acagccttaa
    10861 tgctccttag ataataacca ttatctaaca atgagatacc taattcttag cgtgcctgtt
    10921 aacccacaca tcagggtcta tgcttattaa aagcaggcaa aaagcatgtt ttacggtaaa
    10981 gcccctgcgt ttgctgggtt gaactgataa tcattatcac taacatgctg ttatacgctg
    11041 aaagcgatga agtgaggtaa atcgatggac atgcattcag gaacctttaa cccacaagat
    11101 ttcgcctggc aaggcttaac gctgacaccc gcagcggcga tacacatccg tgagctggtg
    11161 gcaaagcagc cgggtatggt cggcgtgcgc ttaggcgtga agcaaacggg ctgcgcgggc
    11221 tttggctatg tgctcgacag tgttagcgag ccggacaaag acgatctgct gtttgaacac
    11281 gacggcgcga agctgtttgt cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc
    11341 gatttcgttc gtgaaggact taatcagata ttcaaatttc acaaccctaa agcccagaat
    11401 gaatgtggct gtggcgaaag ctttggggta taggcggtac tatgtctcgt aatactgaag
    11461 caactgacga tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa ggattcttca
    11521 cccagttagc caccgatgag ctggcaaagg ggataaacga agaggtggtg cgcgcaattt
    11581 cggcgaagcg taatgagccg gagtggatgc tggagtttcg tctaaacgcc tatcgcgcat
    11641 ggctggagat ggaagaaccg cactggttga aagcgcacta cgacaagctg aattatcagg
    11701 attacagcta ctactcagca ccatcgtgcg gtaattgtga cgacacttgc gcgtctgaac
    11761 ctggcgcggt gcagcaaact ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt
    11821 ttgagcagtt gggcgttccc gtgcgggaag gcaaagaggt ggcggtggat gccattttcg
    11881 actcagtttc ggttgccact acttatcgcg aaaaactggc ggagcaggga attattttct
    11941 gttcctttgg tgaggcgatc cacgatcacc cggaactggt gcgtaaatat ctcggcaccg
    12001 tggtgccggg gaatgacaac ttctttgccg cgcttaatgc ggcggtagcc tctgacggta
    12061 cgtttattta tgtgcctaaa ggcgtgcgct gcccgatgga actttccacc tattttcgca
    12121 ttaacgcgga aaaaaccggg cagtttgagc gcaccattct ggtggccgac gaagacagct
    12181 acgtcagcta cattgaaggc tgttccgctc cggtgcgtga cagctatcag ttacacgcgg
    12241 cagtggtgga agtcatcatc cataaaaacg ccgaggtgaa atattccacg gtacaaaact
    12301 ggttccctgg cgataacaac accggcggta ttctcaactt cgtcaccaag cgtgctttgt
    12361 gcgaaggcga aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca gcgattacgt
    12421 ggaaatatcc cagctgcatt ttgcgcggcg ataactccat tggtgagttt tactcagtgg
    12481 cgctgaccag cggtcatcag caagcggata ccggcaccaa gatgatccac atcggtaaaa
    12541 acaccaaatc gaccattatc tcgaaaggga tctctgccgg acatagtcag aacagttatc
    12601 gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact cagtgcgact
    12661 caatgctgat tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa tgtcgcaata
    12721 acagcgcaca actggagcac gaggcaacga catcacgtat tggtgaagat caactgtttt
    12781 actgcctgca acgcgggatc agcgaagaag acgccatctc gatgattgtt aacggtttct
    12841 gcaaagacgt gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa aaactcctcg
    12901 ccatcagtct tgaacacagc gtcggataag gaataaacat gttaagtatt aaagatttac
    12961 acgtcagcgt ggaagataaa gctatcctgc gtggattaag cctcgacgtt cgtcccggcg
    13021 aagttcacgc cattatgggg ccaaacggtt cgggcaaaag taccttatcg gcaacgcttg
    13081 ccgggcgaga agattatgaa gtgacgggcg gcacggttga gttcaaaggc aaagatttgc
    13141 ttgcgctgtc gccggaagat cgtgcaggcg aaggcatctt tatggccttc cagtatccgg
    13201 tggagattcc cggcgtcagt aaccagtttt tcctgcaaac ggcacttaat gcggtgcgca
    13261 gctatcgcgg ccaggaaacg ctcgaccgct ttgattttca ggatttgatg gaagagaaaa
    13321 tcgctctcct gaagatgccg gaagatttat taacccgttc ggtaaacgtt ggtttttccg
    13381 gcggcgagaa aaagcgcaac gatattttgc aaatggcggt gctggaaccg gagttatgca
    13441 ttcttgatga gtcggactcc gggctggata ttgacgcatt aaaagtggtc gccgatggcg
    13501 tgaactcgct gcgtgatggc aagcgctcat tcatcattgt tacgcactac caacgcattc
    13561 tcgactacat caagcctgat tacgttcatg tgctatatca gggacgaatt gtgaaatccg
    13621 gcgatttcac gttggtcaaa caactggagg agcagggtta tggctggctt accgaacagc
    13681 agtaatgcgc tgcaacagtg gcatcacttg tttgaagctg aagggacaaa acgctcaccg
    13741 caagcacagc agcatttaca acaattgctg cgtaccggac tgccgacacg taaacatgaa
    13801 aactggaaat atacgccgct ggaagggctg accaatagcc agtttgtcag cattgcggga
    13861 gagatatccc cacagcagcg agatgcctta gcgttaacgt tagacgctgt gcggctggta
    13921 tttgtcgatg gacgttacgt gtcagcactg agcgatgcga ctgaaggcag cgggtatgaa
    13981 gtgagcatta acgacgaccg tcagggggta cccgacgcta ttcaggcgga agtgtttctg
    14041 catttgacgg aaagcctggc acaaagcgtg acgcatatcg ccgtgaagcg cggtcaacgg
    14101 ccggcaaagc cattgctgtt aatgcatatt acccagggcg tggcaggtga agaggtcaac
    14161 actgcccatt accgacatca tctggatctg gcggaaggtg ccgaagcaac ggtgatcgaa
    14221 cattttgtca gccttaatga tgctcgtcac ttcaccggcg cacggttcac tatcaatgtc
    14281 gcagcgaacg cccacttgca gcatatcaag ctggcgtttg aaaacccggt cagtcaccac
    14341 tttgcccata acgatttgtt gctggctgac gatgccaccg catttagcca tagtttcctg
    14401 ctgggtggcg cagtgttacg acacaacacc agtacgcaac tcaatggcga aaacagcacg
    14461 ctgcggatca atagcctggc gatgccggtg aaaaacgagg tgtgtgatac ccgtacctgg
    14521 ctggaacaca ataaaggttt ttgtaacagc cgacagttgc acaaaactat cgtcagcgac
    14581 aaaggccgcg cggtatttaa cggtttgatc aacgtcgcgc agcacgccat caaaacggat
    14641 ggtcagatga ccaacaataa tctgctgatg ggcaaactgg cagaagtgga tacgaaaccg
    14701 cagctggaaa tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt ggggcgtatt
    14761 gatgatgaac agatgttcta tctgcgctcg cgcgggatca atcagcagga tgcccagcag
    14821 atgatcattt acgcctttgc tgccgaactg acggaagcac tgcgtgatga ggggcttaaa
    14881 cagcaggtgc tggccagaat cggtcaacgg ctgccaggag gtgcaagatg actttttccg
    14941 tcgacaaagt gcgggccgac tttccggtgc tttctcgtga ggtaaacggt ttgccgctgg
    15001 cttatctcga cagcgccgcc agtgcgcaga aaccgagcca ggtgattgac gccgaggccg
    15061 agttttatcg tcatggctac gcggcggtgc atcgcggtat tcatacctta agcgcccaag
    15121 cgaccgagaa aatggagaac gtgcgcaagc gggcatcgct gtttattaat gcccgttcgg
    15181 cggaagagct ggtgttcgtc cgcggcacga cggaagggat caatctggtc gccaatagct
    15241 ggggtaacag caatgtgcgg gcgggcgata acatcatcat cagtcagatg gagcaccacg
    15301 ctaacattgt tccctggcag atgctttgcg cacgcgttgg cgcagagctg cgtgtgatcc
    15361 cgctcaatcc cgacggtacg ttgcaactgg agacgctgcc tacgctgttt gatgagaaaa
    15421 ctcgcctgct ggcaattact catgtctcca acgtgcttgg cacagaaaat ccactggcgg
    15481 aaatgatcac gcttgcgcac cagcatggcg caaaagtgct ggtggatggc gctcaggcgg
    15541 tgatgcatca tccggtggat gttcaggcgc tggattgcga cttttacgtg ttctccgggc
    15601 ataaactgta tggccccacc ggaattggca ttctttatgt gaaagaagcc ttgttgcagg
    15661 agatgccgcc gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg agtgaaggca
    15721 caacctggac caaagcacct tggcggtttg aagccggtac acccaatacc gggggcatca
    15781 ttggtcttgg cgcggcgctg gagtatgttt cggcgctggg gcttaataac atagccgagt
    15841 atgaacagaa tctgatgcac tacgcgctat cacagctgga atctgtaccg gatctcactc
    15901 tctatggtcc gcagaacagg cttggcgtta ttgcttttaa tctcggtaaa caccatgcct
    15961 atgatgttgg cagttttctc gataattacg gcattgctgt gcgtaccgga catcactgcg
    16021 ctatgccatt aatggcctat tacaacgtcc ctgcgatgtg tcgggcgtcg ctggccatgt
    16081 ataacaccca tgaagaagtg gatcgtctgg tgaccggcct gcaacgtatt caccgtctgc
    16141 tgggataaca gggaggcact atggctgtat tgccggataa agaaaaattg ctgcgtaatt
    16201 ttttacgctg cgccaactgg gaagagaaat atctctacat tattgagctg ggccagcgtc
    16261 tgccagaatt acgcgacgaa gacagaagcc cacaaaatag cattcagggc tgccagagtc
    16321 aggtgtggat tgtcatgcgc cagaatgcgc agggaattat tgaattgcag ggcgacagcg
    16381 atgcggcgat tgtgaaaggg cttattgcgg tcgtctttat tctctacgat cagatgacgc
    16441 cgcaggatat tgtcaatttc gatgtgcgtc cgtggtttga aaaaatggcg ctcacccaac
    16501 atctcacccc atctcgttca caaggtctgg aagcgatgat tcgcgcaatt cgcgccaaag
    16561 ccgctgcact tagctaaact aaagggacag ctttcatccg gttggctcac attgccggat
    16621 ggtcaatgtt tacactgacc tgcacaggag tgtttcggtt tcaggtgaac ataagcgcca
    16681 tccgacgttt cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa acgcgcgtct
    16741 ttgcttacac tcacgcttat cggcgctttt agcgccatcc aggctgcctg ggcggttgat
    16801 tatccgctac caccaaccgg aagccgactg gttgggcaaa atcaaacgta tacggtgcag
    16861 gaaggggata aaaaccttca ggccatcgcc cgacgttttg atactgcggc aatgttgatc
    16921 cttgaagcca ataacactat cgccccggtg ccaaaacccg gtacgacgat aactattcct
    16981 tcgcaactgt tattacctga tgcgccgcgt caggggatta tcgttaacct tgcagagctg
    17041 cgcctttatt attatccgcc gggagaaaat atcgtgcagg tttatccgat aggtattggc
    17101 ttgcaggggc tggaaacgcc ggtgatggaa acgcgtgtgg ggcagaaaat ccctaaccca
    17161 acctggacgc ctactgcagg cattcgtcaa cgttcgctgg agcgtggtat taaattaccg
    17221 ccagtcgttc ctaccggacc aaataacccg ctaggacgtt acgcactgcg cctcgcgcat
    17281 ggtaatggcg aatacctcat tcatggcacc agtgcgccgg acagcgtcgg tttgcgcgtc
    17341 agttcagggt gtattcgcat gaatgcaccg gatattaaag ccttgttctc cagcgtgcgg
    17401 acgggaacgc cggtgaaagt gatcaacgaa cctgtgaaat attccgtgga gcctaacggg
    17461 atgcgttatg ttgaagtaca tcgaccacta tcggcagaag aacagcaaaa cgttcagaca
    17521 atgccataca cactgccagc aggctttacg caatttaaag acaataaggc tgtagatcag
    17581 aagttagtcg ataaagcgtt gtatcgtcgg gcagggtatc cggttgcggt gagcagtgga
    17641 gcaactccca cagccagcaa tgcgccttca gtagagtcag cgcagaatgg tgaaccagag
    17701 caagggaata tgttacgcgc gacgcagtag cggtaaacgg cagacaaaaa aaatggcgca
    17761 caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag tagccatgtt
    17821 gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt cggaacgcat
    17881 tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa cgtcagaaga
    17941 cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac ccaggattac
    18001 cgcgcccagt accagtttag tagctttcat tattaatacc ctctaga
//
LOCUS       DAZFAH010000088        18036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFAH010000088 DAZFAH010000000
VERSION     DAZFAH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.088"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..975
                     /locus_tag="ACHJWB_002757"
     CDS             <1..975
                     /locus_tag="ACHJWB_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4314362.1"
                     /translation="KGIETRDSVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVA
                     QQEPEKVCTACGQSGGGNCPDCGAVMGDATYQETFDEENQVEVQENDPKEMEGAEHPH
                     KENAGSAQDHASDSETGETADPLITVNGHHVITSTSRTSDHLMIDLKTMGKNPDAPII
                     SIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDVIKRWLKQSPEAQSAIMTDEIPLD
                     DALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIV
                     ELGKAIYFDARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            1037..1306
                     /locus_tag="ACHJWB_002758"
     CDS             1037..1306
                     /locus_tag="ACHJWB_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4314363.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            1275..2393
                     /locus_tag="ACHJWB_002759"
     CDS             1275..2393
                     /locus_tag="ACHJWB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4314364.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(2470..2937)
                     /locus_tag="ACHJWB_002760"
     CDS             complement(2470..2937)
                     /locus_tag="ACHJWB_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO4314365.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVDLTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(2915..3118)
                     /locus_tag="ACHJWB_002761"
     CDS             complement(2915..3118)
                     /locus_tag="ACHJWB_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314366.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            3427..4221
                     /gene="potC"
                     /locus_tag="ACHJWB_002762"
     CDS             3427..4221
                     /gene="potC"
                     /locus_tag="ACHJWB_002762"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4314367.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            4218..5264
                     /gene="potD"
                     /locus_tag="ACHJWB_002763"
     CDS             4218..5264
                     /gene="potD"
                     /locus_tag="ACHJWB_002763"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4314368.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEDALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            5322..5783
                     /locus_tag="ACHJWB_002764"
     CDS             5322..5783
                     /locus_tag="ACHJWB_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO4314369.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDSVNIYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            5780..6568
                     /locus_tag="ACHJWB_002765"
     CDS             5780..6568
                     /locus_tag="ACHJWB_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4314370.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(6708..7529)
                     /gene="cobB"
                     /locus_tag="ACHJWB_002766"
     CDS             complement(6708..7529)
                     /gene="cobB"
                     /locus_tag="ACHJWB_002766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4314371.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(7545..8456)
                     /gene="nagK"
                     /locus_tag="ACHJWB_002767"
     CDS             complement(7545..8456)
                     /gene="nagK"
                     /locus_tag="ACHJWB_002767"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4314372.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8485..9729)
                     /gene="lolE"
                     /locus_tag="ACHJWB_002768"
     CDS             complement(8485..9729)
                     /gene="lolE"
                     /locus_tag="ACHJWB_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4314373.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9729..10430)
                     /gene="lolD"
                     /locus_tag="ACHJWB_002769"
     CDS             complement(9729..10430)
                     /gene="lolD"
                     /locus_tag="ACHJWB_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4314374.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10423..11622)
                     /gene="lolC"
                     /locus_tag="ACHJWB_002770"
     CDS             complement(10423..11622)
                     /gene="lolC"
                     /locus_tag="ACHJWB_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4314375.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11884..12957
                     /locus_tag="ACHJWB_002771"
     CDS             11884..12957
                     /locus_tag="ACHJWB_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4314376.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVPAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13085..16531
                     /gene="mfd"
                     /locus_tag="ACHJWB_002772"
     CDS             13085..16531
                     /gene="mfd"
                     /locus_tag="ACHJWB_002772"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4314377.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16675..17637
                     /gene="ldtC"
                     /locus_tag="ACHJWB_002773"
     CDS             16675..17637
                     /gene="ldtC"
                     /locus_tag="ACHJWB_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4314378.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17720..17977)
                     /gene="bhsA"
                     /locus_tag="ACHJWB_002774"
                     /gene_synonym="ycfR"
     CDS             complement(17720..17977)
                     /gene="bhsA"
                     /locus_tag="ACHJWB_002774"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4314379.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4642 a         4608 c         4486 g         4300 t
ORIGIN      
        1 aaggggattg aaacccgcga ttctgtaaac cagaaccagc aagaatcgga acagaacgac
       61 cagaaagcgg aacaaaacag cccaaatacg caacaaaacg agccagaaac gaaacaacct
      121 gagccagtag cgcaacagga accggaaaaa gtctgcaccg cctgcggtca aagcggtggc
      181 ggcaactgcc ctgattgtgg cgcggtgatg ggtgacgcaa cataccagga aacattcgat
      241 gaagagaatc aggttgaagt tcaggaaaat gatccaaagg aaatggaagg cgcagaacat
      301 ccacacaagg agaatgctgg cagcgctcag gatcacgcca gcgatagtga aactggcgag
      361 acggcagatc ccttaattac ggtgaacggt catcacgtta tcacatccac cagcaggacg
      421 agtgaccatc taatgatcga ccttaaaacc atgggaaaaa atcctgatgc cccgattatc
      481 tcaataggtg caatattttt cgatccgcaa accggagata tgggaccgga atttagtaag
      541 actatcgatc tggaaactgc tggcggagtc attgatcgtg acgtcattaa aaggtggctg
      601 aagcaatcac ctgaagcgca gtctgccatt atgaccgatg aaatcccgtt agatgatgca
      661 ctgttacaat tgcgggaatt tatcgacgaa aactccggtg aattttttgt tcaggtctgg
      721 ggaaatggag ccaacttcga taacacgatt ttgcgccgtt catacgaacg gcaggggatc
      781 ccctgcccgt ggcgttacta caacgatcgc gatgtacgca caatcgttga gctggggaaa
      841 gccatatact tcgatgccag aacggctatt ccattcgaag gtgagcgcca taatgcactt
      901 gatgacgccc gttatcaggc aaaatacgtt tcagttatct ggcaaaaact gatcccgagt
      961 caggctgatt tttaatgttc aaccgtcgcc agttgtcgtt ggtattctgc aactggcgcg
     1021 ttccggagtg atagccatga gcgaacagta cctgataacg ctcgatgagt ggaagccaaa
     1081 acggttcagt ctcccaataa caaacactac cctggtgaaa tacggaaaac taggatacat
     1141 cgttccaaga ccacaaaaaa ttcgtgggcg ttggctgata gatcgccgag cagtatttgt
     1201 tggacctggt gaaacaggaa ttgcgccgga aattcatact ggcgatgatg atgcactgaa
     1261 ggagatttta actcatgtca ccgaggccac gaaaaaacag cactgacgta gccggacttt
     1321 acgaaaaatt tgatcgcaga actggcagag tttactacca gtataaaaac cctgtgactg
     1381 gaaaatttca cgggctcgga acagacaaag gtaaggcaga aaaaatcgct tccacagcca
     1441 atcagcgaat agctgcagca gaggctgaat atttcatgcg caaaattgat gaaagtccgt
     1501 cagcaacaaa gcgtcggggt atcagattaa aggcatgggt tgatcgatat ctgaaaatac
     1561 aggacacgcg actgaaaaat ggagatattg cagctacaac tcacaaagaa aaagcccgaa
     1621 tggctgcata cctggtttcc cgtctgggaa accacccatt gaaagaactg gaagtaagag
     1681 actttgcatt aatactggat gagtggctgg ataaagacat ggtcagcaca gcgagagtaa
     1741 atcgtggatt atgggttgat atttataaag aagcacagca tgcaggggaa gttcctcctg
     1801 gatggaatcc tccggaagct acccgtaaac cgatccctaa agtaactaga gccaggctca
     1861 ccctggaaga ctggcaaaaa atttacaacg caacgcctga aaaacacttt atccgtaacg
     1921 caatgcttct tgcgattgtt actggtcagc gccgtgatga catttgccac atgcgttttt
     1981 cagatgtgtg gaacgaacac ttgcatatca cccagggaaa aaccggaatg cgtctggcgt
     2041 taccgcttac actacgctgt gatgccattg ggataacgtt aaaagaagtt attgatgggt
     2101 gccgagacag aatattaagt ccatatctaa tccatagtcg gcaccagaaa caaccaaagc
     2161 cgatgagtaa agacaacctg agcgactact ttgccaaagc acgggatctg gctgggataa
     2221 ctccaccagc aggaaaaact ccgccaacat ttcatgaaca acgctccctg tcagaacgac
     2281 tgtaccgcgc gcagggtatt gatacaaaaa cattactggg acataaagtc caagcaacca
     2341 ccgatcgtta taacgatact cgtggtcagg aatgggttaa attggttgtt tgacgaaata
     2401 aatacagctg aaaaatgttg atattgcttt gctagtgtaa aaaccccggc atcaaccggg
     2461 gttcggagac taatcttgat caggttcctg cttagaataa tgttttccaa caataaatgc
     2521 cgtaaccaat gcaacaagat ctattgaaac gagagttccg gcaagaactg tctcccccat
     2581 gacgccaaag acagtggcag ctaagatgat caatattgcc aaccaaaaag cctttgtttg
     2641 accatctctg gcaatatcaa tgctatcagc cactgtttta tggcgatgag cctgctcttt
     2701 ttcggtcaaa tcaacaagtc gatttgcaag cccaggcacc agttgatcat actttttaag
     2761 cattgaaggt ggcggaacag gcccctgaaa atgctggcaa acaatagccc gcacctgcgg
     2821 actatccaaa accctgttta agacctctgg attttcgatt actcgagaaa ccagttcatt
     2881 atccttttgt tcttcacaag tgagtttggt gttctcactt tctttttgat ctggcattgt
     2941 tcagcttttc aacgttaacg gataccattg ctttttggaa atcgcctctg atagccgcag
     3001 catcctgacg caaagcggaa actggattcg ctggcatgcc atttgctgaa acgaccgtcg
     3061 ggctcagggc tataaccgag ccaatggctt gtaacaaagc cacactttta cgcttcatag
     3121 tcactcctga gttcaatggg attcaaaacg gattcaaagt gaatcttgat agctaattct
     3181 aataccacat tatgtggttt ttcaacacga agaaatcaca tcaaaaatct cattcggaaa
     3241 gcgatatggg taaacaaact ggaatacttt tctgtcctag taagctcacc catgtataaa
     3301 aatgctgtaa aatgcgtggt cctgttttgg ggaaaagttt tggagagatt ttggagaaag
     3361 gaaaaaattg aataaattca aaatcctgaa agtgtcccgc cttatgaata aaaaggtgga
     3421 actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta cgcgtacctg
     3481 tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt tggcatcaac
     3541 tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag cctgttacag
     3601 gcagcgcagc attcactaac aatggcggtg ttttcggcga catttgctac gcttatcggt
     3661 tcactgacgg cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga
     3721 atgctgtttg tggtgatgat gtcaccagat atcgtgatgg cgatttctct gctggtgctg
     3781 tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca tatcaccttc
     3841 tgcctgccgt ttgtggtggt gacggtgtat tcgcgcctga aaggttttga cgtgcggatg
     3901 ctggaagcgg cgaaagatct cggtgccagt gaatttacca ttctgcgaaa aatcattctg
     3961 ccactggcaa tgcctgcggt ggcggcgggc tgggtgttaa gctttaccct gtcgatggac
     4021 gatgtggtag tttcttcgtt cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt
     4081 tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac catattactg
     4141 gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa aacgaaaggt
     4201 aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg
     4261 cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc tacaactgga
     4321 ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt attaaggtta
     4381 tctattcgac ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg
     4441 gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt aaagaaggga
     4501 tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat ccagacatgc
     4561 tcaacaagcc gtttgacccg aataacgact actccattcc ttatatctgg ggtgcgacgg
     4621 cgattggcgt taacggtgat gcggtggatc ctaaatctgt caccagttgg gcagatctgt
     4681 ggaagccaga gtacaaaggt agcctgctgc tgaccgacga tgcccgtgaa gtgttccaga
     4741 tggcgctgcg caaactgggc tactccggta acaccaccga tccgaaagag attgaagctg
     4801 catataacga gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc gataacccgg
     4861 ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggc tctgctttcg
     4921 ttgcacgcca ggcgggtacg ccaattgacg tggtgtggcc gaaagaaggc ggcattttct
     4981 ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaagacgcg ctgaaattga
     5041 tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc cgaaactatc ggttatccaa
     5101 cgccaaacct tgcggcgcgt aagctgttaa gcccggaagt ggcgaacgat aaaacgctct
     5161 acccggatgc tgaaaccatt aaaaatggtg aatggcagaa tgacgttggc gcagccagca
     5221 gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc aagcgggcgg
     5281 tggcattacc gcccgttttt tatttagcaa ggatgtggct aatgagtcag gacagtaaag
     5341 tgttatcccg cgcttttctc ggtatcggtc tgatattgct cgttatttct gcaatcatct
     5401 tttattacca ttttactttt accaaaagcg cggttcatac agaaggaatc attgttgatg
     5461 ccgtttggta caacaatcac tcaaacgacg tcgatgataa tggttcctgg tatccagtgg
     5521 ttgcctttcg ccctacgcca gattacacgc taattttcaa ttcgaatatc ggtagtgatt
     5581 tttatgaaga tagcgaaggt gacagcgtca atatctatta tccgccaggg catccagaac
     5641 aagccgaaat aaacaatccc tgggttaatt tttttaaatg gggctttgtt ggcatagcag
     5701 gcattatttt cggtagtgtc ggtctgataa tcacgctccc ctccaccaaa aaatctcgca
     5761 ggaaacggaa gtcccgccca tgaaaaaatt cattatactg ctcagtttac tgatactcct
     5821 gcctctggta gcaaccagta aaccattgat tccaataatg aagacacttt ttacggatgt
     5881 taccggtact gtaccggatg ctgaagagat cgcacgtaaa gccgagcttt ttcgccaaca
     5941 aacgggaatt gcaccgttta ttgtgatttt acccgacatt aataatgaag ccagtctcag
     6001 acaaaatggt aaagcgatgc tggcgcatgc gtcatcttca ttgagtaatg taaaaggaag
     6061 tgttctgtta ctatttacta cccgcgaacc acggttaatt atgatcacca acggccaggt
     6121 tgaaagtggt ctggacgata aacatctcgg ccttctgata gaaaatcaca cgctggctta
     6181 tttaaatgca gatctctggt atcagggaat caataatgca ttggctgttc tacaagcaca
     6241 gatattaaaa caaccgacgc cgccactgac gtattatccg catccaggac aacagcatga
     6301 aaatgctccc cccggcagca ccaacacatt aggttttatc gcctgggctg cgacgtttat
     6361 cttattttcc tggatctttt gctacacgac tcgttttatt tatgcgttaa aatttgcagt
     6421 ggcgatgact attgccaata tgggttatca ggctttatgt ttgtatatcg ataatagctt
     6481 tgcgattacc agaatatcgc cactctgggc agggctaatt ggtgtctgta cgtttattgc
     6541 cgcattgtta ttgacgagta aacgataaaa gaggccggaa aagtgcgcgt gtattatgcc
     6601 ggatgcggcg cgaacgcctt atccggccca caaaacccgc aaattcaatt aattgcgtcc
     6661 ccttgtagat actgataagc gtagcgcatc aggcaatgct tcctgcttta taatcccttc
     6721 agcaactttt caacaaactc aggcaccacc tggcttgccg ggccgtaata tttctcggca
     6781 aattcattac cgacctgact tggttcgaga ttcagttcca cggtgtgcgc gccatgcagt
     6841 ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc cggaagtacc aatggcaatg
     6901 aaaatatcag ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca
     6961 aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca tttatcttct
     7021 ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg aactttcagc
     7081 aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag gttgtctata
     7141 ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt gcagtttagc cagcgcaaga
     7201 tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc gttataaaac
     7261 gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga
     7321 tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc
     7381 cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac cactttatct
     7441 ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt acgaaaacga
     7501 cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc ggttagatgt
     7561 aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga
     7621 acacgagcta caggtaagag atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc
     7681 gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat ggtcaggata
     7741 tttcccagac aaaccgctaa taaatccaga taacgctcaa cgtgcgccct tgcctgctca
     7801 tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg ataatagtgt
     7861 tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc atgctgacca
     7921 cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc aaccggcaga
     7981 cgcatatggc caaactcgcc ggtaatgtag cttttaccgg taatcggttt gccgttgaaa
     8041 atcagcccgc cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg atattgagta
     8101 aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc aaggcgtaca
     8161 tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt taccgctggc agcggggaca
     8221 ttggcggcat acagcgtacc atcttctgtt tccggcattc ccggaatacc gatgccgaca
     8281 gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac tgcatctaaa
     8341 aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa ctgccgaccg
     8401 ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta atacattgcc
     8461 gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag ggtcaatatt
     8521 actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg ctgtgaccag
     8581 tacgtagaag acgtccagcc agtgcaattc cgatggcagg aaatcaataa aatagatatc
     8641 gctggagagg aactgatgac cgatcaactt ttcaatccac tcaataatcg gggtaagttg
     8701 cagtgaaacc accacaccga taatcacccc acacaggctg ccgaacagcc ctgccagcaa
     8761 tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg ttcttaatac
     8821 tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga tgttgaaaca
     8881 ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca tctggatatc
     8941 gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc tgttggtcac
     9001 ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt taagggcaat
     9061 acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg ggatcatggc
     9121 aaaactgtga tcgagttgac cactcaactg caaaataccg gcaacgtgca aacgcacacg
     9181 ttttggctgc atcagtttat gctcgggatt cgagttgggg atcataatcg acacccaatc
     9241 cccctgcttc actttcagcg catccgccac gcctttgccg atgataattt gctgttcgcc
     9301 cgctttaaaa ttgcgccacg catcgccctg gacaaacgag ggtaatgcgc tcagacgctg
     9361 ttcctgttgc gggttaacgc ccttcacctg gattgcgcga agattcgcgc cactttccac
     9421 cagcccggtg aaattgatat acggcgcggc agcggcaata cccggcactt tttgcacgtg
     9481 atccagtgct tcctgccagt tagtccacgg ctgatccacc gcctcgattt caccatgcgg
     9541 caccaccgcc agaatacggt tgttcagttc gcgttcaaag ccgttcatcg cgcttaagcc
     9601 gacgatcaat accgccacgc cgagggcaat gccaatggta gaaataacgg agatcagcga
     9661 caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg ccaattaata acgataaagg
     9721 catcgccatt actccgcccc catcaggctc agttccgccg tcagacgacc atcacgcatt
     9781 tctaactggc ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg
     9841 gtgccctgca agcgattcaa ttccccaagc aactggaaga tgctgtcggc gttacgcgca
     9901 tcgaggttac cggtaggttc atccgccagt accaggcgcg ggttattcac cagcgcacgg
     9961 gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag atgggcggtg attcgcacga
    10021 tgatccagcc ccaccgcttt taacatctca agtgcacggc tgttgatttc agcgggcttt
    10081 ttcttgccaa tcagcagcgg catagccacg ttttccaggg cggtaaaatc cggcagcagg
    10141 tggtgaaact gataaataaa gcccagcttc tggttgcgca gctctgcttt cgccgccgaa
    10201 gacagtttgc tcatcggttg accgttaaag atcacatcgc cggaggttgg cgtatccagc
    10261 ccgcccaaca agtgcagcaa ggtgctttta ccggaaccag agctaccgac gatcgccatc
    10321 atttcacctt cgccgacgct gaaactgaca ttgtgcagca catcggtttg cacactgcct
    10381 tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat aacgtaaagc
    10441 ctcagcgggt tgagtggcgg cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat
    10501 agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcga ccggcagcgc
    10561 cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc tggcaagcag
    10621 ggcgccaagc gccgctccga ggatcgcacc gataatcccg gcgctggccc cttgcaccat
    10681 aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg ctacttcgcc
    10741 ctgcttctcc attaccatca gccctagtga ggtaataatg ttaaacgccg caacggcgac
    10801 aatcaggctc agcagtaaac ccatcatatt tttttccatg cgtacggcct ggaacaactc
    10861 gcctttacga tcacgccagt cctgccattt gctgccttca ggcagttttt gctgacttaa
    10921 tgagtcgact ttcagcggct catccagcca caaacgccag ccggtaatat tgcctgccgg
    10981 ataacgcatc agacgcgagg catcctcaat attcaccagc atttcattgc catcgacttc
    11041 actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa tacgccccat
    11101 cggcgtgaac tggctggcag atggtaccat catgcggatt tgatcaccgc gattaacgcc
    11161 tagctgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct cgaggtcagt
    11221 ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcgccgggt cgataccgag
    11281 catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg
    11341 tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct gcgggttaag
    11401 agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt tgttttgcag
    11461 ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca ccccgagggt
    11521 aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg cacgcccacg
    11581 catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg tctggttgct
    11641 gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga actgtaagtg
    11701 atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc catgttttat
    11761 catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca gatacccatt
    11821 acacctcggg tcataacccg caggatcaac cgcatgtaaa ttaaaaaata gcgtgttgtc
    11881 ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat ttgtctggtg
    11941 gtgatttatc actcggtcat taccttttat ccgcatctga ccacttttca gcatccgtta
    12001 tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc ctttcgtatg
    12061 ccggtttttt tctttatttc cggctatttg attcgccgct atatcgacag cgtaccgtgg
    12121 ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct ttggggcgtg
    12181 gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg cgatttaagc
    12241 aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg gatgatcacc
    12301 gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt
    12361 tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt ggcggttaat
    12421 ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc ttattacagc
    12481 cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc gttgcgccgc
    12541 catctgctga tggcttcttt gcttaccgtt ccggcggtcg gtgcctggtt gtttactatc
    12601 tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta tcaatacgag
    12661 caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa caccattgct
    12721 atctacacca cccatcgcat tctggttgaa atattcagct taactctgct tgcgcaaatg
    12781 aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta tccctttgtc
    12841 agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc
    12901 agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc ccgctaagcc
    12961 atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat aatgaacagc
    13021 attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga atctgacaac
    13081 cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct
    13141 gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc
    13201 cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat
    13261 cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc ttccctacga
    13321 cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt accagctacc
    13381 gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc
    13441 acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga
    13501 tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg tgatggagca
    13561 cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc
    13621 ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgacag
    13681 ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg aatttccgac
    13741 cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg aagtgaagcg
    13801 cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg ggatcgagta
    13861 ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt tccctgccaa
    13921 taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc aggctgacac
    13981 gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc
    14041 gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc gggtgcagct
    14101 aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc agaaactgcc
    14161 agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta agttcctcga
    14221 gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg
    14281 tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag
    14341 cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg
    14401 taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca
    14461 ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg agctgcatat
    14521 tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa tgaccacgct
    14581 cgaagcgggc ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact
    14641 gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg cggaagaaaa
    14701 cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga
    14761 aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga
    14821 gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt
    14881 tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca tgtgtcagcc
    14941 gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat
    15001 gcgcgccgct ttcctggcag tagataacca caagcaggtg gcggtgttgg tgcctaccac
    15061 ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg
    15121 tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt
    15181 ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa
    15241 gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa
    15301 agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg caacgccgat
    15361 cccacgcacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc
    15421 gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg
    15481 ggaggcgatc ctgcgtgaaa ttttgcgcgg ggggcaggtt tattatctct acaatgatgt
    15541 ggaaaacatc cagaaagctg ccgaacggct ggcagaactg gtgccagaag cgcggatcgc
    15601 catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg atttccatca
    15661 tcaacgtttc aacgtgctgg tttgtacaac cattatcgaa accgggatcg acatcccgac
    15721 agccaacacc attatcattg aacgcgcgga tcacttcggt ctggcgcagc tgcaccagtt
    15781 acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga cgccgcatcc
    15841 aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc tggaagatct
    15901 cggtgcaggc tttgcgctgg caacgcacga tctggagatc cgcggcgcgg gtgaactgct
    15961 tggcgaagaa caaagtggct caatggaaac catcggtttc tcgctgtata tggagttgct
    16021 ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag atctcaccag
    16081 ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcattcctga
    16141 cgtgaatacg cgtttgtcgt tctataaacg tattgccagc gccaaaacgg aaaacgaact
    16201 ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac
    16261 cctgctggat gttgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga
    16321 aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta atccggcctg
    16381 gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgcct
    16441 gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac gccagtttat
    16501 gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc
    16561 atctgcaaca tttacaaatt ctttgcactt ccctgcacta tccgacactg tcaccatcca
    16621 taattcaggc ttatctgttt attacaataa ccttatattt attatggatt tttggtgatg
    16681 atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg
    16741 gcgctaccgg caaaagccaa cacctggccg ctgccgccag cgggcagtcg tctggttggc
    16801 gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa
    16861 tataacgtcg gctttctcgc tctgttacag gctaaccctg gcgttgatcc ttacgtaccg
    16921 cgcgcgggta gcgtgttaac gatcccgttg caaaccctac ttccagatgc gccgcgcgaa
    16981 ggcattgtga tcaatattgc ggagctgcgt ctctattact acccgccggg taaaaattcg
    17041 gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac accgacaatg
    17101 gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga cgccaacggc aaacatccgc
    17161 gcccgttata aagcacaggg aattgagttg cctgcggtag tgccggctgg accggataac
    17221 ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg gcgtttattt gcttcatggt
    17281 acgaacgccg atttcggcat tggcatgcgg gtaagttctg gctgtattcg tctgcgggat
    17341 gacgatatca aaacactctt tagccaggtc accccaggca ccaaagtgaa tatcatcaac
    17401 actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg
    17461 ctgtcagaga agattgatga cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg
    17521 caatcattta aagatgcagc acaaactgac gctgaagtga tgcaacatgt gatggatgtc
    17581 cgttccggga tgccggtgga tgtccgccgt catcaagtga gcccacaaac tctgtaaatc
    17641 ctggataaaa aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg
    17701 cattaatgag ggttaatgct tatttataaa ttactgctgt tccatggagg gtattcggac
    17761 cggttacaga agtaatacgg aaagattttg cgcccatctc atccgctttt tgcgccagct
    17821 gctcttccag cgatcccaga tttgtccccg cgttagcact gattgtaccg actttttgtt
    17881 ggccttctgg cgttgactga acttcgacag ccgcaaagct ggcaaatgac atggagctta
    17941 aaatcgccgc agcgatgagg gtttttacgt ttttcataat agtggcctta tgcagatgaa
    18001 tgacgtcgca agcaattact taacgatcga taacta
//
LOCUS       DAZFAH010000089        17951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFAH010000089 DAZFAH010000000
VERSION     DAZFAH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.089"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..721
                     /locus_tag="ACHJWB_002775"
     CDS             5..721
                     /locus_tag="ACHJWB_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4314380.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            751..1251
                     /locus_tag="ACHJWB_002776"
     CDS             751..1251
                     /locus_tag="ACHJWB_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO4314381.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1343..4027
                     /locus_tag="ACHJWB_002777"
     CDS             1343..4027
                     /locus_tag="ACHJWB_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO4314382.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4024..5112
                     /locus_tag="ACHJWB_002778"
     CDS             4024..5112
                     /locus_tag="ACHJWB_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO4314383.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5200..6498)
                     /gene="exuT"
                     /locus_tag="ACHJWB_002779"
     CDS             complement(5200..6498)
                     /gene="exuT"
                     /locus_tag="ACHJWB_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4314384.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6981..8393
                     /gene="uxaC"
                     /locus_tag="ACHJWB_002780"
     CDS             6981..8393
                     /gene="uxaC"
                     /locus_tag="ACHJWB_002780"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4314385.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8408..9895
                     /gene="uxaA"
                     /locus_tag="ACHJWB_002781"
     CDS             8408..9895
                     /gene="uxaA"
                     /locus_tag="ACHJWB_002781"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4314386.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLKNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9978..10529
                     /gene="ygjV"
                     /locus_tag="ACHJWB_002782"
     CDS             9978..10529
                     /gene="ygjV"
                     /locus_tag="ACHJWB_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO4314387.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(10534..11778)
                     /gene="sstT"
                     /locus_tag="ACHJWB_002783"
     CDS             complement(10534..11778)
                     /gene="sstT"
                     /locus_tag="ACHJWB_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4314388.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12177..13142)
                     /gene="alx"
                     /locus_tag="ACHJWB_002784"
     CDS             complement(12177..13142)
                     /gene="alx"
                     /locus_tag="ACHJWB_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4314389.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     THEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13425..14411)
                     /gene="ygjR"
                     /locus_tag="ACHJWB_002785"
     CDS             complement(13425..14411)
                     /gene="ygjR"
                     /locus_tag="ACHJWB_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4314390.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14490..15173)
                     /gene="ygjQ"
                     /locus_tag="ACHJWB_002786"
     CDS             complement(14490..15173)
                     /gene="ygjQ"
                     /locus_tag="ACHJWB_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO4314391.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15250..15753)
                     /gene="ygjP"
                     /locus_tag="ACHJWB_002787"
     CDS             complement(15250..15753)
                     /gene="ygjP"
                     /locus_tag="ACHJWB_002787"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4314392.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15838..16974
                     /gene="rlmG"
                     /locus_tag="ACHJWB_002788"
     CDS             15838..16974
                     /gene="rlmG"
                     /locus_tag="ACHJWB_002788"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4314393.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17230..17544
                     /gene="higB"
                     /locus_tag="ACHJWB_002789"
     CDS             17230..17544
                     /gene="higB"
                     /locus_tag="ACHJWB_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO4314394.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17541..>17951
                     /gene="higA"
                     /locus_tag="ACHJWB_002790"
     CDS             17541..>17951
                     /gene="higA"
                     /locus_tag="ACHJWB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO4314395.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFI"
BASE COUNT         4774 a         4416 c         4546 g         4215 t
ORIGIN      
        1 ttatatggtg gcgaaaatta atctcttatc acggcttacg ccgcttttat tcgtgttcgc
       61 tccttttttg gcacagtcaa atatgactgt ttatcctatg gctgtttcca ttaatagtca
      121 gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa gttcagtaca tcaaggcgac
      181 agtattccgt atcgataatc catcgacgcc tcaggaaaat gaagttgaga ttaaatccgg
      241 tgatgcaaat catctggttg ttatgccacc taaattcgct ttaccagccg gtagtagcaa
      301 aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag aaaaattatc gcgttaaatt
      361 tgaagcggtt cccagtattg atgacgttgc cacagataaa aaagatctct ctatgcagtt
      421 aacagttaac ttaatttggg ggattgttgt tagtgttcca cctcagcaac ctattgctaa
      481 gttagaagta aatgctgccc aaaaattagt taatgcaggg aatcaacgct taaagatttt
      541 aacgatcgct tattgtaaaa ataatagcaa agaaaattgt aagatacaga ccgtaaataa
      601 aaacatcttc cctggtcagg aaagaaatct tgaaagcata tctggctacg acaaaatcgt
      661 tgtcaaatat aataactgga tcaccaaaga taatggcgag tttgaactgg cggtccatta
      721 attatcattg taataaacga aggaatagta atgaaaaagg tatttgcaaa atctcttctg
      781 gtcgcagcga tgttttctgt tgcaggctcc gcattggcag tacaaaagga tattactgta
      841 acggccaacg ttgatgccgc tctggatatg acacagaccg ataacaccgc gttgccgaaa
      901 gcagtagaaa tgcaatatct gccggggcag ggtctgcaat cttaccagtt gatgaccaaa
      961 atctggtcta acgacgttac caaagatgta aaaatgcagt tagtttcccc ggcgcaactg
     1021 gttcaaagcc tggatgctag caagattgtt ccgctgacag taacctgggg cggtgaagaa
     1081 attaaagctg atgcggcaac cacttttacc gcaaccaaaa tctttgcttc cgatgcgtta
     1141 actaacggtt ctctggctaa aaacctgatg tttgctcaaa ccaccaaagg tgttctggag
     1201 acaggcatct accgtggtgt agtaagcatt tatctgtctc aggatatcta atccagataa
     1261 cagaattaac acgtaacaaa gggaggggta gccctccctt attttaactg ttttagtatt
     1321 tgttatggat ttcaggattg ccatggataa aaaattactg gctcttttga tcctggcgag
     1381 tctcagcccg gcagaggcga cattaaccaa aattcccgca gggtttgagg ttattgctca
     1441 gggacagcag gagtatatcg aggtttattt ttcagggaaa agtctcggta aatattatgc
     1501 aatggttaat cttgataccg taacatttct tgatccagca agtctatata acaagctgga
     1561 actggatgta gacgatcaga aaatcgcgca tatagtgaaa gaaaaattat cgcagccgct
     1621 agctcgccac ggtgaattgg cttgcggtta tgtacgtact gactcagggt gtggttttct
     1681 gaataccgat acgctggaaa taatctataa tgatgaagaa agttcggcaa cgttgtttat
     1741 taatccgcaa tggaattcag ctttcgatgc gaagtcatta tatttaaatc cagacaaaaa
     1801 tactgttaat gcttttatac atcagcaaga catcaatgtt ctggcacagg atgattacca
     1861 atcgttgtct attcagggaa acggtgcgct gggaataaca gaaaatagtt atattggtgc
     1921 acactggaat ttcaacggtt atgatgcaga tgatgtcagt gacagtaatg ctgacgtcag
     1981 cgatctctat tatcgttatg attttttacg tcgttattat gtgcaggcgg gtcgcatgga
     2041 caaccgcaca ctatttaatg cacaaggcgg gaactttacc tttaactttc tgccactcgg
     2101 tgcaattgat gggatgcgta tcgggtcgac actgagctat ttaaaccagg cgcaaagcca
     2161 gcaaggaacc ccagtaatgg ttctgctttc gcgcaattct cgtgttgacg cttatcgtaa
     2221 tgagcaactt ttgggatcgt tttatctcaa tagtggttcg caatttattg ataccagttc
     2281 ctttccgcca ggcagctaca gcgtggcatt aaaagtctac gaaaataacc aactcacccg
     2341 caccgagctt gtgccgttta ccaaaaccgg cggtctgact gacggaaatg cgcaatggtt
     2401 cttacaggca ggtaaaacta catcacaggt ttctgatgat gaaagttcag cttatcagct
     2461 aggagtacgc ctgccattac atccgcaata tgagctctac gcagggctgg cgaatgccga
     2521 tgatgtgagt gctttcgagt taggtaataa ctggacggca gatttaggcg gggtggggaa
     2581 tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc ggcaaaggtg atatgcaaca
     2641 ggccaactgg agtaatccgg gatggccgac gttgggcttt tatcggacca actctgacgg
     2701 cgatgcttgt acaaccgaca gcagagagag ctataacgcc ttaagctgtt atgaaagtat
     2761 ttccgcgacg gtttcacaga attttgtcgg ctggaatatg atgctgggtt atacccgcac
     2821 acaaaataac actgatgata gtttgcgttg ggataaacag cagagctttg aaaataacta
     2881 tcttcgccag acaacagcgc aaagtatctc tgaaactgta caacttagcg cttcccgcgc
     2941 ttttgtgatg cgtgactgga ttttgagtac gtcagtcggt gttttccatc gtaatgacaa
     3001 cggtggcgat aacgacgaca acggcttgta cttatcgttt tcgttatctg acacgccaac
     3061 gatggatagc aataacaaca gccattcaac aaatgtgtct acggattatc gttatagcga
     3121 acaggatggc gatcaaacgt catggcagtt atcgcatacc ttttataacg attcattcag
     3181 ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt
     3241 taacgggcgt tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga
     3301 ccgtaagaat catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt
     3361 cagtcgctat ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtact
     3421 ggtggatgtg aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc
     3481 gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt gacagtgtgt tgttccctta
     3541 tcctggattt cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg
     3601 gacaacaaac atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct
     3661 gcgctatcgc gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc
     3721 ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga
     3781 agatggcgga tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg
     3841 gcagcaattc cttaagtgtc cactgagtgt tgtaaagaaa cgcgccagca ttcgttacag
     3901 cggagatgtt acttgtagtg tggtgactta ttcacaatta ccggagtcga ttcaggttca
     3961 ggcacagttg aaacagccta aattacgtgg aaacgttcag acggcgcaaa gggaggttgc
     4021 accatgagaa atcgattgat tgcggcgata ttgggcttgt ttggcacgct cactggcgtt
     4081 caggcagctc ctgacgtgac cagtgaaatt acgtatgatt tggcatctgg tagagcggat
     4141 tattacttct ggaaggatga agcgtcggca ggaaataatg gatatatgtg gtatgaatgt
     4201 tcgtatcctg acctccaaca aacctgtaca gctaatggaa atatatcgac agtacaaatc
     4261 tatttaactg aacaacgcag tgggatgcgt tggccggtaa aactcaaagg atttaaaaca
     4321 gccattgtaa gtagcgatga agcgccgcca ggatgcaagg ggggcaaagg gcttcagacg
     4381 aatcttaagg attctaatag atcttcatgt acagaagatg gtcaacatta ttatatatac
     4441 gatacaaagt ttcttacgct ctaccttgag cagacagaga tgaagaattt gccgattggt
     4501 ggcgtctgga aggggaaagt taaattacat tcgaacagcc cggcccagga ctatttcgca
     4561 aatattaccc tgaatacgct cgaccccaac catattgacg tgttcttccc ggagttcgcc
     4621 cacgccacgc caagggtgca gttagacttg catccaacag gaagcgttaa cggcagcaac
     4681 tacgcgcaag atctgaccat gttggacatg tgcctgtacg atggttttaa cggtaatgcc
     4741 atcagttatg aaatcatgct caaagatgaa gggcgaccag ctgcagggcg cagagacggt
     4801 tacttctcta tctatcgtca gggagggacc accaccgacg agggagaacg cattgattac
     4861 cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg atgtgcgcaa taatgaaaat
     4921 atggtctgga acagcattaa cctgaaacgt gtgcgtccgg tggtactgcc cggtattcgc
     4981 tatgccgtga tgtgtgtgcc gacacccttg actctggcag tcgataaatt cagcgtgatg
     5041 gataaacagg ccgggtacta catgggcaaa ttgtcagtaa tctttacgcc ttccttgcca
     5101 accatcaatt aaccctgcaa accggacaaa cgcttttttc tatataaacg acgaagccgt
     5161 ctgataaagc ggcttcgtca ttacaactta agttaaacgt taatgttgcg gtgcgggatc
     5221 gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc tgcaacacgg tccagataac
     5281 cagcgcacct aacaggtcga acactgccag aactgcgaac agcgggctga agccgatggt
     5341 gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc
     5401 ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat
     5461 cagcgcgcca gacagtgcct ggtgagcaaa accaccgata cacagcagca taattgcgac
     5521 atacgggttg gtgaacaaac cgatcatacc cgggccaatc atcagcacag cacccagcgt
     5581 tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg
     5641 caggtaacca ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat
     5701 cgcgatctct ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt
     5761 aaaagtaccc caggccggtt ctgccaggaa acgcggcagc gcgatacccc agaactgacg
     5821 gttacgcagg atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc
     5881 ctgaccatta ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg
     5941 tttatagaaa atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa
     6001 tgccatctgc cagctgtgca ttacgattgc ccataccacc agcggcggcg caatcatcgc
     6061 accaatcgaa gaacctacgt taaagtaacc taccgcgatg gaacgctctt tcgccggaaa
     6121 ccattcggag ctggctttca gacccgccgg gatcatcgct gcttccgcgg caccgaccgc
     6181 accacgagca acagccaggc caccccagct acctgccagc gcggttgcac cacagaacac
     6241 ggcccacagc acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata
     6301 acctgctacc ggttgcatga ccgtataagc agcagaatag gctgcgatga tataggaata
     6361 ctgttgggtg gagatgttta actcttccat caaagttggc gcagctgccg ccacagtgtt
     6421 acgcgtcagg taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa
     6481 ccctttaatt ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc
     6541 atcatcccgc cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca
     6601 cgccccgaac gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga
     6661 ttcatctaac aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg
     6721 cagaaagata acttgtcata caactttaaa aggtgagagc catcacaaat gtgggaatat
     6781 ttgtagggac attacctgac gacagcgagg ccagtactgg cgcggcctgc agcgagattt
     6841 accactttga gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc
     6901 ggactctgga aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac
     6961 tcgttgagag gaagacgaaa atgactccgt ttatgactga agatttcctg ttagataccg
     7021 aatttgcccg ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt
     7081 gccatttgcc gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct
     7141 ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc
     7201 tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc
     7261 acactatcgg caacccgtta taccactgga cgcacctcga actgcgtcgt ccgtttggta
     7321 tcactggcaa attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac
     7381 tgctggcgca ggataacttc tccgcgcgcg gcatcatgca gcagatgaac gtgaaaatgg
     7441 tcggcaccac cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagacg
     7501 gctctttcac catcaaagtg ctgccgagct ggcgtccgga caaagccttc aacatcgaac
     7561 aggcgacctt taacgactac atggcgaagc tgggcgaagt ttccgatacc gacattcgcc
     7621 gctttgctga cctgcaaact gccttgacca aacgtctgga tcacttcgcc gctcacggct
     7681 gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc tgaagcgaac gaagcggaac
     7741 tggacagcat tctggcgcgc cgtctggctg gcgaaaccct gagcgagcac gaagtggcac
     7801 agttcaaaac tgcggtactg gtgttccttg gtgccgaata tgcacgtcgc ggctgggtac
     7861 agcagtacca cattggcgcg ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc
     7921 cggatgtagg ctttgactcc atcaacgacc gtccgatggc ggaagagttg tctaagctgt
     7981 tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat tctctactgc ctgaacccgc
     8041 gcgataacga agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca
     8101 aaatgcagtt cggttccggc tggtggttta acgatcagaa agacggtatg gaacgtcaga
     8161 tgacccaact ggcgcagctc ggtttgctga gccgctttgt cggtatgctg actgacagcc
     8221 gtagcttcct gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgatcg
     8281 gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa cctgctgggc gagatggtga
     8341 aaaatatttg ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg
     8401 ggttgatatg caatacatca agatccatgc gctggataac gtcgcggtcg ctttagcaga
     8461 tttggctgaa ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt
     8521 tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa ggggccaacg tcatcaaata
     8581 tggcctgccg attggttatg cattggcgga tattgcggcg ggggaacacg tccacgccca
     8641 caatacgcgc acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga
     8701 tctgcctgcg caagcggcag atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt
     8761 cggggtgcgt aatgagctgt ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg
     8821 gcaaatccag aaccgtttcc tgaaagagac caacaacgcc gaaggtaccg acggcgtgtt
     8881 cctcttcagc cacacctacg gctgttcaca actgggcgac gatcacatca acacccgcac
     8941 catgctgaaa aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg
     9001 ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg
     9061 cgttcatttc atgatctgcc aacagcagga tgatgagatc gaagccggga tcgagcattt
     9121 gcatcagctg tataacgtga tgcgcaacga taaacgcgag ccaggcaaac tcagcgaact
     9181 gaagtttggt ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat
     9241 gctggggcgt ttctctgact acgtgattgc taacggcggt actaccgtac tgaccgaagt
     9301 gccggagatg tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt
     9361 tgaaaaactg gtcaccatgg tcaacgactt taaacagtac tttattgccc atgatcagcc
     9421 gatctacgaa aacccatcac cggggaacaa agcgggcggt atcaccacgc tggaagacaa
     9481 atcacttggc tgtacccaga aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg
     9541 cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc
     9601 taccagcgcc ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgcggcac
     9661 gccatacggt ggatttgtgc cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa
     9721 gaaaaaacac tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca
     9781 gttgctggaa gaatttatcg acaccatcgt tgagtttgcc aacggtaagc aaacctgcaa
     9841 cgagcgtaac gacttccgcg aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg
     9901 aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata
     9961 cctgctaagg atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct
    10021 ttctgattgg tatcacaaca tttttcaatc gtgacgaacg tcgcttcaaa aagcagcttt
    10081 cggtctatag cgccgttatt ggcgtacatt tttttcttct gggcacctat cccgctggtg
    10141 ccagtgccat ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg
    10201 taatggcgat ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg
    10261 tcgaactatt gccggttatc ggtacgattg tcagtacctg ggcgctgttc cgctgtaaag
    10321 ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg ctgggtgatt cacaacttct
    10381 gggcggggtc gataggcggc acgatgattg agggtagttt tctgcttatg aatggcctga
    10441 atatcattcg tttctggcgg atgcagaaaa ggggaattga tccgtttaaa gtagagaaaa
    10501 cccctcccgc catagacgaa aggggttaaa caattaatta cgcagggcgc tatttgccag
    10561 acgatcgtct tccgcctggc aagctgccgc agtgaacagc acgtcagttg aagagttcag
    10621 cgcggtttcg caagagtcct gcaatacgcc gataataaag ccgacggcaa ccacctgcat
    10681 ggcgatatcg ttcgaaatac cgaacatatt acaggccagt gggatcagca gcagagaacc
    10741 ccccgccacg ccggatgcgc cacaggcaca cagagaagcc actacgctca acagcagcgc
    10801 tgtgggcaga tcgaccggaa tacccagcgt attaaccgca gccagcgtca acacggtaat
    10861 ggtgattgct gcgcccgcca tattgatggt ggctcccagc ggaatagaaa cggaataggt
    10921 atcgcgatcc agattcagct tttcacacag cgccatattc accggaatgt tcgctgcaga
    10981 gctgcgggtg aagaaggcat acacaccgct ttcgcgcagg cacagcagca ccagcgggaa
    11041 cgggttacga cgaattttcc accacaccag caatgggtta accaccagcg ccaccagtaa
    11101 catacagcca accagcacga ccagcagttg cgcgtagccc cacagtgtgg agaaaccggt
    11161 ggttgccagg gtagaagaaa ccagcccaaa aataccaatc ggtgcgaagc gaatgaccag
    11221 tttcaccata aaggtaacgg cattcgacat atcgttaacc aggtttttgg tggtctcgtt
    11281 accgtgacgc agtgcgaagc cgaggccgat cgcccacacc agaatcccga tgtagttacc
    11341 tttcagcagc gcgtcgatgg ggttggaaac catgctcatt accagcccgc gcatcacttc
    11401 gacaatgcct gacggcggcg aaatatcacc cgcgctactg gataaatgca gggtagaagg
    11461 gaaggcaaag ctgaagacta ctgcggccag agcagcagag aaggtgccca gtagatagag
    11521 gaacaaaata gggcggatat tggttttctg cccgtgctgg tggttagcaa tagatgccat
    11581 taccagcatc aacaccagga tgggggcaac ggctttcagt gcgccgacga acaaagtacc
    11641 taacagacca acagcttccg ccgcgggttt tgagatccat gccagaagaa tccccagaac
    11701 aaggccgacc aggatttgtt ttaccaggct gccatgagct agacgccgga atagccccgg
    11761 tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt gtcgcacatc cctggtgcgt
    11821 tctgtcgacg catctttcag gatgtaacaa agtgtttgca aggtcgagta taaggaaagt
    11881 tacagcttcg ggaagcgaaa aatgctggat tttaaagaga ggtcatattt tgcaacttaa
    11941 tggttacatt tatttgacat aaaaataaag ccaacgtgcg gcttccagac tgatgacaac
    12001 atcaaaaaag gactcggtca gtcccctcgc cccgtttggg gagagggtta gggtgagggg
    12061 aacaggccag catcggtgcg aaaaattacc ctcaccccga ccctctccct ggaagggaga
    12121 gggagaaaac ggtgctcatg ttaatgacag ggtacacggc ttaggcagat taaaaattat
    12181 ccaccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc
    12241 agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc
    12301 agcatcttga taccgataaa caccaggatc accgccaggc catatttgag catcgagaaa
    12361 cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat cgcaaacagg
    12421 tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc
    12481 acggcgaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag cggtgtggcg
    12541 tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc
    12601 aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg
    12661 gtgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag
    12721 tcgaactgcg aaattagcca gctgccagtg aagatcatga tggtacgcag aacaatcgcc
    12781 ccgagcacgc catacaccag cacgcggcgt tgtaatgccg ccggaacaga gaaatagctg
    12841 aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat cagataacct
    12901 gtgagaaagg ccagtgcctg tggatcggca acggcgcgac cttcggtttg caccagatac
    12961 caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca
    13021 gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc
    13081 agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc
    13141 atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc cgctgctttt
    13201 tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc
    13261 gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc
    13321 gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagccag
    13381 tcaatggtct gttacatacc ttttacactg ttttactttg gcaattatag ttttacgcta
    13441 tctgccggaa aaatcacccc agtctggcgg cggatctcgg tcagcagttt ggcggtgatg
    13501 cgactgaccg ccagccccgg atgatccacc agatgctcat ccaccagggt agcgaacagc
    13561 tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg catttggctg
    13621 ccacgcggca cgaagcacac tttctggcat tcagacagtt tttcaatcac cagcgatcct
    13681 gcttcgccct gaatctcgct cgccaggaca gaatcactga cttttgagtg ctgcaaggtg
    13741 acgctgaaat caccgtaatc catcaccacc acgccgtggg catctacacc gcttgccagc
    13801 aaactggcgg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc cgacgccagg
    13861 cagtaaaagc cgatatccat aattgaaccg ttagagaatg acggattaaa ggtgttggga
    13921 ttttcaccgt ccaggtaacg ttgatagcgc gaggaatact ggcaatagtt gaaaaagact
    13981 ttacgcaatt tgccgacttt cggcagcgcc tggcgcagca aatgaaagtt cggcaggcag
    14041 gcggttttaa atgcctcaaa cagcaccacc tgattttccc gcgcacaggc aatggcggca
    14101 tccacttccg ccagattcga cgccagtggt ttctcgcaaa tcacatgaat tttatggcta
    14161 aggaaaagtt gtgtctggga aaagtgcagg gcattcgggc tggcaatata caccgcgtca
    14221 atggcatcgc tttccgccat cgcttccagc gaggtaaaca gatgctcgac agaaaaatca
    14281 ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa cttgtattta
    14341 ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg
    14401 aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct
    14461 ttttcatctt cgacgtgcgc agatacgcat tattcttttg ccggacagcc atgctcacta
    14521 aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag gattttggcg
    14581 tcaatcacgg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc
    14641 atattgaggt caggcgcgtt aaaaccgata gcgtcgatgc tgtactgttt tgccagccag
    14701 atggcgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc accaaaaacc
    14761 tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca gaagatcact
    14821 ttcgccggta cgcctttagc gatcaaagcc ttctgcatac ctgatgcttc atcgtaattt
    14881 ttacgtccgt tatcgccact taccagtagc catttcactt ttccggcgtg gtacaacgct
    14941 gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctggcccc cagcaataag
    15001 cccacgttac gctccggaac tgcgttgaca tcgctccagg tcagctgctt gctggcgttg
    15061 accataacgc gatcggcaat aaaaatggtc gctcctgcta taagcaacaa caaacagcca
    15121 atcttaagag tccggcgaga aaaacaaata cggagaagaa ggcggggctg catggtcgtt
    15181 atccttaacg aaagggggga ggtcgatttt aggcgctaaa gaaagaaaag ataacgcctg
    15241 acatcatttt cagattttgt cctgcccaag cgacaactgc gttagccaca gccgggtgtc
    15301 gaactcaagc tggtggtact gcggctccat atggcagcac aattgataga acgctttgtt
    15361 atgctctttc tccttcaggt gcgccagctc gtgcaccacg atcatgcgca aaaacggttc
    15421 cggcgcgttg cgaaacacgg tagcgacgcg gatctccgct tttgctttca gcttgccgcc
    15481 ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt tttagcacat ggatcttatt
    15541 gtcatacatc actttattga tcggcggggc attgcgcaga aactgatttt ttagatcctg
    15601 agtatattgc cagagggctt tatcggtggc gtagtcgtgt gttcccggat agcgttttgc
    15661 cagcacatcg cccagacgct gttcgttaat cagcgttcgc acctgggaaa gtagctgctc
    15721 gggataaccc tgaagataag taagattgct cataaccgcc cacgaaaaag agaaaaaggg
    15781 tatactcacg cacccttttc aggggcaacg tcgaaatttt atcattcagg agggccgatg
    15841 agccacttag acaacggttt ccgttcactg acactacaac gttttccggc gacggatgac
    15901 gttaacccgc tacaggcgtg ggaagcggcg gatgaatatt tgctgcaaca gttggacgac
    15961 acagaaatcc gcggcccggt gttgatcctg aatgatgcct ttggtgcgtt aagctgcgcg
    16021 ctggcggaac ataagccgta cagcattggc gactcataca tcagtgaact ggcgacgcgc
    16081 gagaatttac gcctcaacgg gattgatgag tcgagcgtga agtttctcga cagcaccgcc
    16141 gactacccgc aacagccggg tgtggtgctg atcaaagtgc cgaaaacact ggcattgctg
    16201 gaacaacaac tgcgtgcgct gcgcaaagtg gtcacgtcgg atacacgtat tattgccggt
    16261 gccaaagccc gtgacattca cacttccacg ctggaactgt tcgaaaaagt gctcggcccg
    16321 accaccacca cgctggcatg gaaaaaagcg cgcctgatta attgcacgtt caatgaaccg
    16381 ccgctggccg atgcgccgca gaccgttagc tggaagctgg aaggtactga ctggactatc
    16441 cataaccatg cgaatgtctt ctcccgcacc gggcttgata tcggcgcgcg cttctttatg
    16501 caacatctgc cagagaatct cgaaggggag attgtcgatc tcggttgcgg taatggcgtt
    16561 attggtctga cgctgcttga taaaaacccg caggcgaaag tggtgtttgt cgatgaatcg
    16621 ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca acatgccaga ggcgttggat
    16681 cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg agcctttccg ctttaatgct
    16741 gtgctctgca acccgccgtt tcaccaacaa catgcgctga ccgataacgt cgcctgggag
    16801 atgttccacc atgcccgccg ctgcctgaaa atcaacggcg agctgtatat tgttgccaac
    16861 cgtcacctgg attacttcca taaactgaag aagattttcg gcaactgcac caccatcgcc
    16921 acgaataata aatttgtggt gctgaaagca gtgaagctgg ggcgtcgtcg gtaagattgg
    16981 gagttacctg ccggatgcgg cgttaacgcc ttatccggcc tacgttcagc acgttactcc
    17041 caggccggat aagacgcggc aagcgtcgca tccggcaatg tctgcccagt tctggaatcg
    17101 cgcttccata aaacaaaaaa tccctctcac catcctctac attctcttgt ttagcgtttt
    17161 tctacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg tcaacggacg
    17221 tgatgaagaa tgcacctgat aactcaaaaa gcattgaaag atgctgcgga aaaatacccg
    17281 caacataaaa cggagttggt ggctctgggg aacacgattg ctaagggata tttcaaaaaa
    17341 cctgagtcat taaaagcagt attcccatct ctggataact tcaaatatct ggataagcat
    17401 tatgttttca atgttggggg caatgaatta cgtgttgtag caatggtctt ttttgaatcg
    17461 caaaagtgct acatacgtga agttatgacg cataaagaat acgatttctt taccgctgtt
    17521 catcgtacta aggggaaaaa atgattgcga ttgccgacat cttgcaagca ggagaaaagc
    17581 taactgctgt ggcacctttt ctggcgggta ttcagaacga ggaacaatac acccaggcgc
    17641 tggaactggt agatcatctg ctgctcaacg atcctgaaaa ccccttgctg gatctggtgt
    17701 gtgcaaaaat aaccgcgtgg gaagaatcag cgcccgaatt tgcggaattt aatgccatgg
    17761 ctcaagccat gcctggcggt atagccgtga ttcgtaccct tatggatcaa tatggtttaa
    17821 ccctttccga tctgccggaa attggcagta aatctatggt gtcacgcgtt ttgagcggga
    17881 agaggaaatt aacgctggaa cacgctaaaa aattggcaac gcgattcggc atttctcccg
    17941 ccttgtttat t
//
LOCUS       DAZFAH010000090        17922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFAH010000090 DAZFAH010000000
VERSION     DAZFAH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.090"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..2785
                     /gene="adhE"
                     /locus_tag="ACHJWB_002791"
                     /gene_synonym="adhC"
     CDS             110..2785
                     /gene="adhE"
                     /locus_tag="ACHJWB_002791"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4314396.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            2909..3805
                     /locus_tag="ACHJWB_002792"
                     /pseudo
     CDS             2909..3805
                     /locus_tag="ACHJWB_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4087..4704)
                     /gene="tdk"
                     /locus_tag="ACHJWB_002793"
     CDS             complement(4087..4704)
                     /gene="tdk"
                     /locus_tag="ACHJWB_002793"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4314397.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     gene            5309..5722
                     /gene="hns"
                     /locus_tag="ACHJWB_002794"
     CDS             5309..5722
                     /gene="hns"
                     /locus_tag="ACHJWB_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4314398.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5866..6774)
                     /gene="galU"
                     /locus_tag="ACHJWB_002795"
     CDS             complement(5866..6774)
                     /gene="galU"
                     /locus_tag="ACHJWB_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4314399.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(6976..7989)
                     /gene="rssB"
                     /locus_tag="ACHJWB_002796"
     CDS             complement(6976..7989)
                     /gene="rssB"
                     /locus_tag="ACHJWB_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4314400.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8081..8986)
                     /gene="rssA"
                     /locus_tag="ACHJWB_002797"
     CDS             complement(8081..8986)
                     /gene="rssA"
                     /locus_tag="ACHJWB_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4314401.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9099..9557
                     /locus_tag="ACHJWB_002798"
     CDS             9099..9557
                     /locus_tag="ACHJWB_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4314402.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGTIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9607..10449
                     /gene="purU"
                     /locus_tag="ACHJWB_002799"
     CDS             9607..10449
                     /gene="purU"
                     /locus_tag="ACHJWB_002799"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4314403.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10609..10693
                     /locus_tag="ACHJWB_002800"
     tRNA            10609..10693
                     /locus_tag="ACHJWB_002800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10643..10645,aa:Tyr,seq:gta)
     gene            10741..10872
                     /locus_tag="ACHJWB_002801"
     ncRNA           10741..10872
                     /ncRNA_class="other"
                     /locus_tag="ACHJWB_002801"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11056..11733)
                     /gene="narI"
                     /locus_tag="ACHJWB_002802"
     CDS             complement(11056..11733)
                     /gene="narI"
                     /locus_tag="ACHJWB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4314404.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11733..12443)
                     /gene="narJ"
                     /locus_tag="ACHJWB_002803"
     CDS             complement(11733..12443)
                     /gene="narJ"
                     /locus_tag="ACHJWB_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4314405.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12440..13978)
                     /gene="narH"
                     /locus_tag="ACHJWB_002804"
     CDS             complement(12440..13978)
                     /gene="narH"
                     /locus_tag="ACHJWB_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4314406.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(13975..17718)
                     /locus_tag="ACHJWB_002805"
     CDS             complement(13975..17718)
                     /locus_tag="ACHJWB_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4314407.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4440 a         4739 c         4330 g         4413 t
ORIGIN      
        1 agtaagcttt tgattttcat aggttaagca aatcatcacc gcactgacta tactctcgta
       61 ttcgagcaga tgatttacta aaaaagttta acattatcag gagagcatta tggctgttac
      121 taatgtcgct gaacttaacg cactcgtaga gcgtgtaaaa aaagcccagc gtgaatatgc
      181 cagtttcact caagagcaag tagacaaaat cttccgcgcc gccgctctgg ctgctgcaga
      241 tgctcgaatc ccactcgcga aaatggccgt tgccgaatcc ggcatgggta tcgtcgaaga
      301 taaagtgatc aaaaaccact ttgcttctga atatatctac aacgcctata aagatgaaaa
      361 aacctgtggt gttctgtctg aagacgacac ttttggtacc atcactatcg ctgaaccaat
      421 cggtattatt tgcggtatcg ttccgaccac taacccgact tcaactgcta tcttcaaatc
      481 gctgatcagt ctgaagaccc gtaacgccat tatcttctcc ccgcacccgc gtgcaaaaga
      541 tgccaccaac aaagcggctg atatcgttct gcaggctgct atcgctgccg gtgctccgaa
      601 agatctgatc ggctggatcg atcaaccttc tgttgaactg tctaacgcac tgatgcacca
      661 cccagacatc aacctgatcc tcgcgactgg tggtccgggc atggttaaag ccgcatacag
      721 ctccggtaaa ccagctatcg gtgtaggcgc gggcaacact ccagttgtta tcgatgaaac
      781 tgctgatatc aaacgtgcag ttgcatctgt actgatgtcc aaaaccttcg acaacggcgt
      841 aatctgtgct tctgaacagt ctgttgttgt tgttgactct gtttatgacg ctgtacgtga
      901 acgttttgca acccacggcg gctatctgtt gcagggtaaa gagctgaaag ctgttcagga
      961 tgttatcctg aaaaacggtg cgctgaacgc ggctatcgtt ggtcagccag cctataaaat
     1021 tgctgaactg gcaggcttct ctgtaccaga aaacaccaag attctgatcg gtgaagtgac
     1081 cgttgttgat gaaagcgaac cgttcgcaca tgaaaaactg tccccgactc tggcaatgta
     1141 ccgcgctaaa gatttcgaag acgcggtaga aaaagcagag aaactggttg ctatgggcgg
     1201 tatcggtcat acctcttgcc tgtacactga ccaggataac caaccggctc gcgtttctta
     1261 cttcggtcag aaaatgaaaa cggctcgtat cctgattaac accccagcgt ctcagggtgg
     1321 tatcggtgac ctgtataact tcaaactcgc accttccctg actctgggtt gtggttcttg
     1381 gggtggtaac tccatctctg aaaacgttgg tccgaaacac ctgatcaaca agaaaaccgt
     1441 tgctaagcga gctgaaaaca tgttgtggca caaacttccg aaatctatct acttccgccg
     1501 tggctccctg ccaatcgcgc tggatgaagt gattactgat ggccacaaac gtgcgctcat
     1561 cgtgactgac cgcttcctgt tcaacaatgg ttatgctgat cagatcactt ccgtactgaa
     1621 agcagcaggc gttgaaactg aagtcttctt cgaagtagaa gcggacccga ccctgagcat
     1681 cgttcgtaaa ggtgcagaac tggcaaactc cttcaaacca gacgtgatta tcgcgctggg
     1741 tggtggttcc ccgatggacg ccgcgaagat tatgtgggtt atgtacgaac atccggaaac
     1801 tcacttcgaa gagctggcgc tgcgctttat ggatatccgt aaacgtatct acaagttccc
     1861 gaaaatgggc gtgaaagcga aaatgatcgc tgtcaccacc acttctggta caggttctga
     1921 agtcactccg tttgcggttg taactgacga cgctactggt cagaaatatc cgctggcaga
     1981 ctatgcgctg actccggata tggcgattgt cgacgccaac ctggttatgg acatgccgaa
     2041 gtccctgtgt gctttcggtg gtctggacgc agtaactcac gccatggaag cttatgtttc
     2101 tgtactggca tctgagttct ctgatggtca ggctctgcag gcactgaaac tgctgaaaga
     2161 atatctgcca gcgtcctacc acgaagggtc taaaaatccg gtagcgcgtg aacgtgttca
     2221 cagtgcagcg actatcgcgg gtatcgcgtt tgcgaacgcc ttcctgggtg tatgtcactc
     2281 aatggcgcac aaactgggtt cccagttcca tattccgcac ggtctggcaa acgccctgct
     2341 gatttgtaac gttattcgct acaatgcgaa cgacaacccg accaagcaga ctgcattcag
     2401 ccagtatgac cgtccgcagg ctcgccgtcg ttatgctgaa attgccgacc acttgggtct
     2461 gagcgcaccg ggcgaccgta ctgctgctaa gatcgagaaa ctgctggcat ggctggaaac
     2521 gctgaaagct gaactgggta ttccgaaatc tatccgtgaa gctggcgttc aggaagcaga
     2581 cttcctggcg aacgtggata aactgtctga agatgcattc gatgaccagt gcaccggcgc
     2641 taacccgcgt tacccgctga tctccgagct gaaacagatc ctgctggata cctactacgg
     2701 tcgtgattat gtagaaggtg aaactgcagc gaaaaaagaa gccgctccgg ctaaagctga
     2761 gaaaaaagcg aaaaaatccg cttaatcagt agcgctgtct ggcaacataa acggcccctt
     2821 ctgggcaatg ccgatcagtt aaggattagt tgaccgatcc ttaaactgag gcactataac
     2881 ggcttccaca acagggagcc gttttcttat gccacttctc aatgatctgc tcgatttcag
     2941 tgaccatccg cttatgcctc cgccctctgc acaactattt gcagaacacc ttcccaccga
     3001 gtggatacaa cactgcctga cgctttctgc tcatgcgacc gttcgccgcc gtcgtttacc
     3061 gggggacatg gttatctgga tggtggtgca atgagccaat taccgatgtt gttcgccgtc
     3121 tgaacctgag cgcggatggc gaagcgggga tgaacctgct ggccctcagc gctgtcaccc
     3181 aggcgcgtca gcgcgtgggg gccgccccag tggaatggct cttccgccag accgcacaga
     3241 caggggcgcg gaacgttacc tgaaggatga ctggcacggc ctgcaacttt ttgccattga
     3301 tggcgcacag ttcaggacac ctgataaacc cgagctgcgt gaatattatg ggtctgccaa
     3361 cacatccact aagcggcaga acgcctaccc ggttatgcgt ctggtggcct taatgaacct
     3421 gggaagtcac attctgctga atgccgtgac cgcaccttac cggcagagcg aaactgtgct
     3481 ggcccactcc atgctcgcca ccatcccgga taactctatt acgttgtttg ataaactttt
     3541 ttacagcgaa gacctgctgc tgacgctgaa ccagaagggt tgtaaccgcc actggctact
     3601 gcctgcgtgg aagaatatcg catcagaaat gatagagctg ggaaacacgg cgtcaccagg
     3661 gaccatcccg aaacgactgg agcatcttcg gggtgctctg gaagtggtgt tcataacaaa
     3721 acgcccccga ccatcaaggc cgaggtcggt taagatatca aaaacccgtt atcctgtgaa
     3781 acatagtgcc gctcctctta agtgaacggc attgcccttc tggggccgta ttttttgttt
     3841 acccaaagca acttttccat aaaccgacag cattagcctt catcatattt gcgacgatgt
     3901 ataacgccta aacacaggga tattgtactt tacaggtcac aagtcaacgt cggtgcttaa
     3961 gagccctgtg aggcgtatag cggcgttaaa aaactgccga gaagggtata tagcccggaa
     4021 gaagtgcgta aaacgaactg acaggataaa agtgccctgc tcaccctgtc agtaaagaaa
     4081 ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagtcga cttgtaacgc
     4141 ctctttatag tgtttacggc atacagaaac gtatcgttca ttaccaccaa ttaccacctg
     4201 ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc ttgctttacg
     4261 gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca gtaagtattg
     4321 gctgccaata aataattcac ctcgaaaatc ggtacgtaaa ccataacaaa gtacgggtat
     4381 atctagttga tcgacaacct ccgataattc atatacttgt tgtctggtta aaaactggca
     4441 ttcatcaacc agtacgcaat gaattgcctg ctgttcatgt tccgcacgaa tctcatcaaa
     4501 taatgatgaa ttttggttaa ataattttgc aggcgatgac aaacctatac gcgaactgac
     4561 tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc gcatgccgcg
     4621 ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat tcattgcgga
     4681 atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa ataaagttgg
     4741 ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga agtgggtata
     4801 tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca actgcctcgc
     4861 ttgttataag cgggtaaatg actgctggta actattcaca atctttaacc tgttgcgcaa
     4921 gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccatg ctcttattgc
     4981 gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacaca actaatacag
     5041 aagactgaaa ggtcgtcagc ctacgataat ctccccataa aatgtgacat gaatcaggaa
     5101 gttttaacct cacgtgctgc gaaatcatcg gtgtaaatag ggctatatgc cgcgtctttt
     5161 ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca attttgaatt
     5221 ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc tcaacaaacc
     5281 accccaatat aagtttgaga ttactacaat gagcgaagca cttaaaattc tgaacaacat
     5341 ccgtactctt cgtgcgcagg caagagaatg tacacttgaa acgctggaag aaatgctgga
     5401 aaaattagaa gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg ctgctgaagt
     5461 tgaagagcgc actcgtaagc tgcagcaata tcgcgaaatg ctgatcgctg acggtattga
     5521 cccgaacgaa ctgctgaata gccttgctgc cgttaaatct ggcaccaaag ctaaacgtgc
     5581 tcagcgtccg gcaaaatata gctacgttga cgaaaacggc gaaactaaaa cctggactgg
     5641 ccaaggccgt actccagctg taatcaaaaa agcaatggat gagcaaggta aatccctcga
     5701 cgatttcctg atcaagcaat aatcttttgt agattgcact tgcttaaaat cccgccagcg
     5761 gcgggatttt ttattgtccg gtttaagaca atttaataag aataagccat aaaaaaacgg
     5821 cgtcgattgc tcaacgccgt ttcgtggata acaccgatac ggatgttact tcttaatgcc
     5881 catctcttct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta
     5941 ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat
     6001 atgataggct tccaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat
     6061 ttcatcacca gctcccggag gggtttttgc cagcaacggc caaatatccg cgctaagtac
     6121 gtaacgaccc acaatagcga gattagacgg cgcaacatcc gctttcggtt tttctaccac
     6181 accaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc
     6241 atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa
     6301 gcggcggatc atctctgcca ggttatcctg tgacaaatcg gattcatatt catccagaat
     6361 aacatcaggc aaaataacag ctaccggttc atcacccact accgggtgag cacacaatac
     6421 cgcgtgtccc aggcctttcg ccagaccctg acgaacttgc ataatagtca cgtgcggtgg
     6481 acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc
     6541 cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt gtgtaaccag
     6601 cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt
     6661 atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc
     6721 taatcccgca acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat
     6781 tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc
     6841 agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac
     6901 ttatacgaat ggcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc
     6961 cgcgttatcg tttgctcatt ctgcagacaa catcaagcgc agtcgaccac cggttcccca
     7021 tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa
     7081 agtgcctaac ggaacaccat tactgatttg cacctggtgt tcgccggtat ttaacgtcgc
     7141 attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa
     7201 tagcggaaac tgccccggca gattggcctg acggagtaaa tggtttacct gcttcaataa
     7261 cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt
     7321 aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc gggtgacatc
     7381 aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg
     7441 tttgtccgcg gcaaccaatt gacgataatt aacccggcaa tgggaaatca cctgctgaac
     7501 cggcggttgt agttcctgta ataatttcgc cgctgcggca gggttatcaa ccattgcatc
     7561 ccagtcgcga aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag
     7621 acaggcaaaa accatctcgc gcaagcgatt cagatcttta actggtttca gcaaaacatc
     7681 ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcggtgg cagatatcac
     7741 cagaaccggg gtctggtcgc ctctgttacg tatatgctcc agcagtttaa gcccgttcat
     7801 tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag
     7861 ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accacgaatc
     7921 cagaagcgag cgaaataccg gctcatcttc aacaatgaga atctgttttc cgaccaatgg
     7981 ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccacctg
     8041 cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca
     8101 acggcaaaag ttcgtccatt ttcttttcca ctgccagctg tccggccgca atggcagcgt
     8161 gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg
     8221 gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg
     8281 tcataatctc tgttgccgtt ggtgctgtca ccgcccgacg tgtcgttatg ctgcctaacc
     8341 tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga
     8401 cattaaagga gagcaaatct tgttgcatta aatgagcatc gtgctgcagg tcgaccgcta
     8461 tcacaatatc agcacccaat gcacgcgtga gcgaaatagg aattgggtta acgactgccc
     8521 catcaaccag ccagtagccg ttatgcgcga ctggtgccat gagtcctgga atactgcatg
     8581 acgcgcgaat agcaagatgg agatcgcctt cagtaaacca taattcacgc ccagtactta
     8641 aattggtggc aacagccgcg aagcgacggg aacaattttc gatctctgtt tccggcatta
     8701 tctcgcggta ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga
     8761 gatccatcag gcgtaaaaca tcccaataac tgaaagaggt tacccaatct tccagcgcag
     8821 ataatcgatc gcatgcatag gccgcgccca ccagcgaacc aattgaacat cctgcaacga
     8881 tatcaatttc aatacccact ttttttagcg cattaataac gccaatatgc gaccaacctc
     8941 tcgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat
     9001 tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg
     9061 cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg
     9121 gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac
     9181 ctgatccaga acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt
     9241 atttaataaa gacctggcat ccatcttgtg gagcagcggc gttacgtgcc gaattgattg
     9301 ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgt tggcaggacg
     9361 ccgacaatat cggatttgtc agttttgttg ctcgatttac cgaaggtggc aaaacaggta
     9421 caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta
     9481 cacgtccgca gtttggccgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa
     9541 agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt
     9601 ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt
     9661 ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt gcagaacaat
     9721 gaatttgttg atcaccgtac cggacgcttt tttatgcgca cggaactgga agggattttt
     9781 aatgattcca ccctgctggc ggatctcgat agcgcattgc cagaaggctc ggtgcgtgag
     9841 ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt
     9901 ggcgatttgt tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt
     9961 ggtaaccacg atactttacg ttctctggtt gagcgttttg atattccgtt tgagctggta
    10021 agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggacgc cattgatgct
    10081 tatcaacctg actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg
    10141 gcacgcttcc cgaataaaat catcaatatt caccattcat tcctgccagc gtttatcggc
    10201 gcacgtcctt atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc aaccgctcac
    10261 tatgtgaatg acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat
    10321 catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta
    10381 agtcgtgcgc tatacaaagt actggcacag cgcgtctttg tttacggtaa tcggacgatt
    10441 attctttaat cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac
    10501 gcaaccagtt catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc
    10561 gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg tggggttccc
    10621 gagcggccaa agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct
    10681 tcccccacca ccatcacttt tcaaaagtcc gtaaactctc aaacgaatcc gcaattaaat
    10741 attctgccca agcggggaag gatgagaagc ttcgaccaag gttcgacttg agcgccagcg
    10801 agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg agtaatcctt
    10861 cccccaccac catcgcttcc ttaaataatc aaaacctgtt actccgagct aattttgccc
    10921 gatggcacca cgtttaccgg gcctacaata tcctcccctg taggtcggat aagcgaagcg
    10981 catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc agggtctatg
    11041 tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg tcagatactc
    11101 caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga ataacgtcat
    11161 ccccagcacc aggtgcagac ggaagataaa cgccacacca tcgaggtgtt gagaagcgcc
    11221 accgtggaag gtcaccaccg actgcgccca gccaaccagt ttcatcatct cgctaccgtc
    11281 catatgctga gcggagaacg gaatggtcaa caggcccagc gcgcactgga taacgagcag
    11341 cgacaggatc aggatatccg ctccggtagt ggttgcacgc acgcgtgggc tgaacagacg
    11401 acgcttcagc aacagcacgc cgccaatcag acacagcacg ccgctggcac caccagcaaa
    11461 catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc agtgcggcgt
    11521 cagcataccg aagaagtgac caacaaaaat ccccagaata ccgatatgga acaggttcga
    11581 cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc aggtgtactg
    11641 cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta tgtacgggta
    11701 gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt ggtgatattc
    11761 agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat tgctgaatca
    11821 ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccagacagc atccagcgcc
    11881 tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc gctgtcgata
    11941 gcggtattgg ccaatttcag cagcagatca aacatcacgg cataacggct ttcacgctgt
    12001 tgcagacgcg cgctcagcaa tgccagaatt ggcgcgatat ctttcaaacc ttccacggct
    12061 tcgctttgcg gcagctgcga caggtactcc agatacagcg gcagatggtc cggtaattcg
    12121 cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac catcgcctgg
    12181 ccgcggtcac gggattcacc gtgaacatgt tcaaatagca acagtgacgt ggcgcggcca
    12241 cggtcgaaca gttcgctgta ctgggcctgg gcatcgagcg gatccatcgc cgttaaatcg
    12301 cgcaggaaaa tgcccagcgc atgggcatct tctttcgaca gatttttcga cgcagcaatc
    12361 gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc aaggagacgc
    12421 gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca tcgatggcat
    12481 cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag ccatcaccaa
    12541 aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca cgatgactac
    12601 tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac atctcctgtg
    12661 cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta ccgtcaacgg
    12721 tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagtgcgcgc agtaccggtt
    12781 tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc aagctttcga
    12841 cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac tgaatcggag
    12901 acagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc agcggcagcg
    12961 ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcggct tcaataacgc
    13021 tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc ggatcgagga
    13081 acacctccag ctgacgctgg taaagatctt tctcgttctc ggtgctggct gcacgttcaa
    13141 tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca caggtttctg
    13201 agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac ttctcagact
    13261 taccgctctt ccagttgaag tagatttttt tgtacgggca tccggtgatg cacatacgcc
    13321 agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc ttgtaaatcg
    13381 caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac aggcgcggca
    13441 aatacatcat aaaggtgttt tcgaactggc tatacatcgc cttctggatg ttgtcgaagt
    13501 tcttgtcttt cgccagtttg tcaaactcac cacccaggtc atcttcccag ttcggccctt
    13561 tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca atcggctgcg
    13621 atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac ggctcgtaat
    13681 aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcaac atggcacggt
    13741 tacccatgcg cggctgcagt ttgccgttga ttttacggat ccagccgcct ttgtattttt
    13801 cctggttttc ccagtcagtc gggaagccct gacccggctt ggtttccacg ttgttgaacc
    13861 acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact gaacaggtgt
    13921 ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga attttcattt
    13981 tacgctctcc tgtacctggt cattgccttc gccatctaac cagtcaatgt tcttcatctt
    14041 acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt taaagccgta
    14101 tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac gggtgaccga
    14161 gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa cgatacgttc
    14221 ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa ccgcacgggc
    14281 agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga tacccagatc
    14341 tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag tcagcatcag
    14401 caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca ggaagttgag
    14461 cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg aacgggtgtc
    14521 gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact ggtgatcctg
    14581 atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa cgttggtgta
    14641 accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc tggagataat
    14701 tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt tattcagcgc
    14761 cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt tcacggctac
    14821 ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa ttgcggtgtt
    14881 cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct tacgcagcag
    14941 atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccattaccga ttttctccat
    15001 cagcgggccg atagaggtaa agcgttcgta agtcgccgga taatcgcgct ctacgaccat
    15061 aatgtgtggc gcggttttac ctgggatcag gtcgcactcg ccttttttcc agtctttcac
    15121 atccagcggc tgcgccagtt cagcggcaga gtcatgctgg ataggcagcg tgacgatgtc
    15181 ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga tggctttgta
    15241 gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca gcgggtgaat
    15301 aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg ccgtcggcaa
    15361 aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa ccaccagatc
    15421 cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca cgccgccctg
    15481 ttgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact tgagcataaa
    15541 ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagatgaaca ggttgcgcgg
    15601 gtggttttta ccgttttctg gttgttctgc cgcaaaacgg atggaaccct ctttcaggga
    15661 tttcaccgta tagtccaccg gattcatccc ggctttttcc gcttcgccag cgatagtcag
    15721 cgggttagtg cctaactgcg gtgcagacgg cagccagccc atgcgttccg cacggacgtt
    15781 aaagtcgatc aagtgtccgg tatagcggga tttgtccgcc atcggtgaca gcaactcttc
    15841 cgccgtgacg gtttcataac gccactggct ggagtggtta tagaaataag aagtgctgtt
    15901 catgtgacgc gccggacgct gccagtcaag ggcaaacgcc agcggctgcc agccggtttg
    15961 cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct gaccgacaca
    16021 gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt gataccagtg
    16081 gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag cgttatcggc
    16141 aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct gctcggccca
    16201 ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt cgttcaggcc
    16261 acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca gggcggtgct
    16321 gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt tttccagttc
    16381 cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta cctcagcgat
    16441 ctcatcctgg ctacccagca ggctcagttg cagctcggtt tcttcgccag ttttgccgtc
    16501 gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag aaccgttcgg
    16561 cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat tgttttcctg
    16621 gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt agtaaccgtc
    16681 gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat agtcggtgaa
    16741 atactggctt gggttgtcga ggtggaattc acgcagcatt acgtggccca tcgccagcgc
    16801 cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt tggcgatttc
    16861 agcgtagtct ggtgtgacgg caacagtttt ggtccctttg taacgcactt cagtaaagaa
    16921 gtgagcatcc ggggtacgcg tctgcggtac gtttgacccc caggcgatga tgtagctgga
    16981 gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt gcggagacgc
    17041 aggaggcaag tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa tcagcgagag
    17101 atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg agaaaccagc
    17161 aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga tcagttcgtt
    17221 cacctcctgc caggaagaac gaacaaatcc accgcgtcca cgcgcctgct taaagctttt
    17281 cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc tatgcagcgc
    17341 cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt tcaggcggtt
    17401 ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag gttcatggtt
    17461 tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg tgaccagacc
    17521 gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt gggtagagcg
    17581 gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac ggttggtatt
    17641 gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt agcgaaaccg
    17701 gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc tgataaatcg
    17761 acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat tgataacgat
    17821 caagtgtgat ggggatgaaa aataaagtaa attcctaaga taccccttta ggataattaa
    17881 cagttaaagc ataacatgct gtaaattaag acgttttata cc
//
LOCUS       DAZFAH010000091        17596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFAH010000091 DAZFAH010000000
VERSION     DAZFAH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.091"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..1265
                     /gene="yhiM"
                     /locus_tag="ACHJWB_002806"
     CDS             213..1265
                     /gene="yhiM"
                     /locus_tag="ACHJWB_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4314408.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1582..2784)
                     /gene="yhiN"
                     /locus_tag="ACHJWB_002807"
     CDS             complement(1582..2784)
                     /gene="yhiN"
                     /locus_tag="ACHJWB_002807"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4314409.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3016..4515
                     /gene="pitA"
                     /locus_tag="ACHJWB_002808"
     CDS             3016..4515
                     /gene="pitA"
                     /locus_tag="ACHJWB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4314410.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4586..4921)
                     /gene="uspB"
                     /locus_tag="ACHJWB_002809"
     CDS             complement(4586..4921)
                     /gene="uspB"
                     /locus_tag="ACHJWB_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4314411.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5312..5746
                     /gene="uspA"
                     /locus_tag="ACHJWB_002810"
     CDS             5312..5746
                     /gene="uspA"
                     /locus_tag="ACHJWB_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4314412.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5845..5898)
                     /locus_tag="ACHJWB_002811"
     CDS             complement(5845..5898)
                     /locus_tag="ACHJWB_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314413.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6064..7533
                     /gene="dtpB"
                     /locus_tag="ACHJWB_002812"
     CDS             6064..7533
                     /gene="dtpB"
                     /locus_tag="ACHJWB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO4314414.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7582..8334)
                     /gene="rsmJ"
                     /locus_tag="ACHJWB_002813"
     CDS             complement(7582..8334)
                     /gene="rsmJ"
                     /locus_tag="ACHJWB_002813"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4314415.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8342..10384)
                     /gene="prlC"
                     /locus_tag="ACHJWB_002814"
     CDS             complement(8342..10384)
                     /gene="prlC"
                     /locus_tag="ACHJWB_002814"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4314416.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10587..11429
                     /gene="rlmJ"
                     /locus_tag="ACHJWB_002815"
     CDS             10587..11429
                     /gene="rlmJ"
                     /locus_tag="ACHJWB_002815"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4314417.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11501..12853
                     /gene="gorA"
                     /locus_tag="ACHJWB_002816"
     CDS             11501..12853
                     /gene="gorA"
                     /locus_tag="ACHJWB_002816"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4314418.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12907..12990)
                     /gene="dinQ"
                     /locus_tag="ACHJWB_002817"
     CDS             complement(12907..12990)
                     /gene="dinQ"
                     /locus_tag="ACHJWB_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4314419.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13730..14083
                     /gene="arsR"
                     /locus_tag="ACHJWB_002818"
     CDS             13730..14083
                     /gene="arsR"
                     /locus_tag="ACHJWB_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4314420.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14137..15426
                     /gene="arsB"
                     /locus_tag="ACHJWB_002819"
     CDS             14137..15426
                     /gene="arsB"
                     /locus_tag="ACHJWB_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4314421.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15439..15864
                     /gene="arsC"
                     /locus_tag="ACHJWB_002820"
     CDS             15439..15864
                     /gene="arsC"
                     /locus_tag="ACHJWB_002820"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4314422.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15998..16294)
                     /locus_tag="ACHJWB_002821"
     CDS             complement(15998..16294)
                     /locus_tag="ACHJWB_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4314423.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16401..17402
                     /locus_tag="ACHJWB_002822"
     CDS             16401..17402
                     /locus_tag="ACHJWB_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4314424.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLQAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         4078 a         4505 c         4440 g         4573 t
ORIGIN      
        1 gtaaaaaaaa acaacagaat aagaattaat cttaggataa atatttattt atcatggcct
       61 tttaaccggt aaaaaatggc actcggcaca gtaaaccccg ctatagttat gctatgcatt
      121 tgtctttaat gatatctcgt cgttatatta acaggctgaa attatcatta atgcacattt
      181 cattattagc agggatacct gaggagttta aagtgaacat atatatcggg tggcttttca
      241 aattaatccc tttgattatg ggattaattt gcatcgcgtt aggtggcttt gtgctggaaa
      301 gttcagggca aagcgagtat ttcgtcgcgg gtcatgtgct gatttctctg gcggccatat
      361 gcctggcatt attcactacc gcatttatta tcatttcgca gctcacgcgc ggcgttaata
      421 cgttttacaa tacattgttc cccattattg gctatgcggg gtcaattatc accatgatat
      481 ggggttgggc actgttagca ggcaatgatg tgatggcaga cgagtttgtc gccggccatg
      541 ttattttcgg cgttggtatg attgccgcct gtgtatcgac ggtggcagcg tcatccggtc
      601 actttctgct cattcccaaa aatgcagcgg ggagcaagag cgatggaacg ccggtacagg
      661 cttattcttc attaatcggt aattgcctca ttgccgttcc cgttttactc accctgctcg
      721 gtttcatttg gtctattacg ctgttacgta gtgctgacat aactccgcat tatgtcgcgg
      781 gtcacgtatt gcttgggtta accgcaatct gtgcctgtct aattggcctt gttgccacaa
      841 ttgtccatca aacacgtaat acgttttcaa ctaaagaaca ctggctgtgg tgttattggg
      901 ttatttttct cggctcaatc acggtactgc aggggatata cgtcttagtc agttccgatg
      961 caagcgcccg actggctccc ggcattattc ttatttgcct cggaatgatc tgttacagca
     1021 tattctcaaa agtctggcta ctggcactgg tctggagacg tacctgttcg ttagccaaca
     1081 gaataccgat gatacccgtc ttcacctgcc tgttttgcct tttcctggca tcatttcttg
     1141 cggaaatggc gcagaccgac atgggatatt ttattccttc gcgagttctg gtcggtttgg
     1201 gagcggtatg ctttacgttg ttctcaatcg tttcaatatt agaagcgggt tctgctaaaa
     1261 aataattgca acgtaccgga taaaaccagc gttgaccatt tgcgtaacgc tggtttttct
     1321 taggcatcat gaaataacgc agcattaatg catagtggtt aaggataaaa aaaacaacaa
     1381 agttactgtc tttttcagcc tgttcatatt ttataaagat ctggccactg ggtgagtttt
     1441 caatcacctt tccttccacc ttatattaag cctggagggt ttcagttcac gggctcatta
     1501 gaaaataatc acaagatagc cactatcaaa attagctcat ttttaatgcg gctctaataa
     1561 ttttcatctt taggtaacag gtcaggacga cttcgctgca atcaaatcct gcgcacaagc
     1621 ccatgcactc gaccacgccc actggaagtt atagcccccc agccagccgg tgacgtccat
     1681 cacttcgccg atgaagtaca gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag
     1741 ttcgttggtg tccacgccgc cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt
     1801 gggttgtacg cgccagtcgg tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag
     1861 ctgttttagc gaaacatccg ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt
     1921 cggtagatga accgccagtg tgtttttcag gctctgattc ggatgtgcgt tacgctgctc
     1981 attcaggaag gtttcgaggt ccacatccgg tagcagattg atactgacaa attcacccgg
     2041 ttgccagtag ctggaaatct gcaacaccgc cggtccagac aagccgcgat gggtgaagag
     2101 taagttctca cggaaaacgg tgccgttttc agcggtaatc acggaaggca ccgccacgcc
     2161 cgccagcacc tgtaactctt cgagcaacgg tttatgcaga gtgaacggca ccagacccgc
     2221 gcgggtcggc agcacgttga ggccaaattg ttcggcaatc ttataaccaa acggcgatgc
     2281 gcccagcccc ggcattgaca gcccaccggt cgcgatgacc agcttttcgc aaccgacagt
     2341 catgccgttt agatcaagcg tgaagcctgt ttcatccttc gccacactca gcacttcgct
     2401 acgcaatctg aaggtcacat tgcccttctc gcactcatcc accagcatgt cgacaatctg
     2461 ctgcgcggag tcatcgcaga agagttgccc taacgttttc tcgtgccatg cgatgccgtg
     2521 tttattgacc agatcaatga aatcccactg ggtaaaacgt gcgagtgcag acttacaaaa
     2581 atgcggattc tggctcagat aagcgcctgg ttcgacataa aggttggtaa agttgcagcg
     2641 cccaccgcca gacataagga ttttgcgccc tggtttttta ccattatcga tcagcagaac
     2701 ccggcgtcct gcctgacctg ccagcgcaga acagaacata cccgccgcac cagcgcctat
     2761 aataatggca tcaaaccttt ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt
     2821 aaagattcct cagtggtcgc accagcatca atattactaa aaggaagtat ttgcctgaat
     2881 tatataagat aattattttt tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta
     2941 tatttcactt tgcccgcgcc gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa
     3001 atggcgtaac gtcctatgct acatttgttt gctggcctgg atttgcatac cgggctgtta
     3061 ttattgcttg cactggcttt tgtgctgttc tacgaagcca tcaatggttt ccatgacaca
     3121 gccaacgccg tggcaaccgt tatctatacc cgcgcgatgc gttctcagct cgccgtggtt
     3181 atggcggcgg tgttcaactt tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc
     3241 attgtgcata tgctgccgac ggatctgctg cttaatatgg gatcgtctca tggccttgcc
     3301 atggtgttct ctatgttgct ggcggcgatt atctggaacc tgggtacctg gtactttggt
     3361 ttacctgcat ccagctctca tacgctgatt ggcgcgatca tcgggattgg tttaaccaat
     3421 gcgttgatga ccgggacgtc agtggtggat gcactcaata tcccgaaagt attaagtatt
     3481 ttcggttctc tgatcgtttc ccctattgtc ggcctggtgt ttgctggcgg tctgattttc
     3541 ttgctgcgtc gctactggag cggcaccaag aaacgcgccc gtatccacct gaccccagcg
     3601 gagcgtgaaa agaaagacgg caagaaaaag ccgccgttct ggacgcgtat cgcgctgatc
     3661 ctttccgcta tcggcgtggc gttttcgcac ggcgcgaacg atggtcagaa aggcattggt
     3721 ctggttatgt tggtattgat tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc
     3781 actggctacg aaatcacccg tacccgtgat gccatcaaca acgtcgaagc ttactttgag
     3841 cagcatcctg cgctgctcaa acaggctacc ggtgctgatc agttagtacc ggctccggaa
     3901 gctggcgcaa cgcaacctgc ggagttccac tgccatccgt cgaataccat taacgcgctc
     3961 aaccgcctga aaggtatgtt gaccaccgat gtggaaagct acgacaagct gtcgcttgat
     4021 caacgtagcc agatgcgccg cattatgctg tgcgtttctg acactatcga caaagtggtg
     4081 aagatgcctg gcgtgagtgc tgacgatcag cgcctgttga agaaactgaa gtccgacatg
     4141 cttagcacca tcgagtatgc accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt
     4201 atcggtacga tgattggctg gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag
     4261 aaaggcatga cctacgctca ggggatgtct gcccagatga cggcggcagt gtctatcggc
     4321 ctggcgagtt ataccgggat gccggtttcc actactcacg tactctcctc ttctgtcgcg
     4381 gggacgatgg tggtagatgg tggcggctta cagcgtaaaa ccgtgaccag cattctgatg
     4441 gcctgggtgt ttacccttcc ggctgcggta ctgctttccg gcggactgta ctggctctcc
     4501 ttgcagttcc tgtaatcgta cacaccaaaa cgagcgggtc agtttactgg cccgcttttt
     4561 ttatgcgtta ttattgctga agtgcttaat gccaaatcat caatgcaatc aggctgacca
     4621 ccaccagacc acacaatgcg ctggtcagaa taaactgccg acgcacccgc tcacagcggc
     4681 gaataaattc atcatcgtga tgatcgcgat aacgttgggc atagatatac caaacgagac
     4741 gcacctgttt gttgggttgg ccatgtgagg taaaaaagcc ccctccatca acatattgat
     4801 agagcaatgg atcgcagtta cgcagtacca ctaacaacgc gcgtagtgat gagaaatagc
     4861 gcgccatgtt aacaatgcaa acgacacata aagcccaaaa taatgcgacg gtgcttatca
     4921 tacctcctcc ccggcgacct gcccgcggag ttccaccccg gggctaccgc tcccgatacg
     4981 ctgccaatca gttaacacta ggtcctggag aaaccgcttt tgtggtgacc aacatccgag
     5041 cggctctata gatagtgtag gagatcaggt tgtttttttt ccagaaggtt aaccactatc
     5101 aatatattca tgtcgaaaat ttgtttatct aacgggtaag caaggcggat tgacggatca
     5161 tccgggtcgc tataaggtaa ggatggtctt aacactgaat ctttacggct gggttagccc
     5221 cgcgcacgta gttcgcagga cgcgggtgac gtaacggcac aagaaacgct agctggccag
     5281 tcatcgacaa ctttatggaa ggagtaacac tatggcttat aaacacattc tcatcgcagt
     5341 cgatctctcc ccggaaagca aagttctggt agagaaagca gtctctatgg ctcgccccta
     5401 caatgcgaaa gtttctctga tccacgtaga tgtaaactac tctgacctat acaccgggct
     5461 tattgatgtg aatctgggtg atatgcagaa acgcatctct gaagagacac atcatgcgct
     5521 gaccgagctt tccactaatg caggctaccc aatcactgaa accctgagcg gcagcggcga
     5581 cctgggccag gttctggtcg atgcaatcaa gaaatacgat atggatctgg tggtttgtgg
     5641 tcaccaccag gacttctgga gcaaactgat gtcttccgca cgtcagctga tcaacaccgt
     5701 tcacgttgat atgctgattg ttccgctgcg cgacgaagaa gaataatcat ccctccacga
     5761 cgtgttcctg aacgcccgca tatgcgggcg ttttgctttt tggcgcgcct tgttacctga
     5821 tgcagcgtaa accccttatc tggcctacga tctgcgtacg caatcaaaat ctcctgccaa
     5881 tacaacattt aacaccatca tattttccat cattagtgtg atcagctggt tattttctgt
     5941 tgtaatagtg tattaatcta ttcaccgcat caatattaag aatctctgac agatgtaaac
     6001 tttttcgcgc gttatccctt acgcgttcat acttttcagg atggtattgg aaggttaata
     6061 aatatgaata caacaacacc catggggatg ctgcagcaac ctcgcccatt tttcatgatc
     6121 ttttttgtcg agttatggga gcgattcggc tactacggcg tgcagggcgt actggcggtt
     6181 ttcttcgtta aacagcttgg attctcgcaa gagcaggctt ttgtcacttt tggtgctttt
     6241 gctgcgctgg tctatggcct catttccatt ggcggctatg tcggcgacca cctgctgggg
     6301 accaaacgca ccattgttct cggagcactt gtgctggcga ttggctactt catgaccggc
     6361 atgtcgctac ttaagcctga cctgattttc atcgccctgg ggactatcgc tgtcggtaac
     6421 ggcctgttta aagctaaccc agccagcttg ctttcgaagt gctatccgcc gaaagatccg
     6481 cggcttgatg gcgcattcac cctgttctat atgtcgatca atatcggctc gttgatagcg
     6541 ttatcgctgg cccctgtgat cgctgataga ttcggttatt cagtcaccta caacctgtgc
     6601 ggggcggggt taattatcgc attactggtt tacatcgcct gtcgtggaat ggtgaaagac
     6661 attggttctg aacccgactt caagccgatg agcttcagca aactgttgta cgtattactt
     6721 ggcagcgtgg tgatgatctt cgtatgcgca tggctgatgc acaacgtaga agtcgccaat
     6781 ctggtgctga ttgttctctc catcgtcgtc accatcatct tctttcgtca ggcattcaag
     6841 ctggataaaa ccgggcgcaa taaaatgttt gtcgcctttg tcctgatgct cgaagcggtg
     6901 gtgttttaca ttctctacgc ccagatgcca acatcgctga acttctttgc catcaacaac
     6961 gtgcatcatg aaattctcgg tttttccatc aacccggtca gcttccaggc gcttaacccg
     7021 ttctgggtgg tactcgccag cccaatactg gcaggcattt acacgcatct gggtaacaaa
     7081 ggcaaagacc tctcgatgcc gatgaaattt actctcggca tgtttatgtg ctcactgggc
     7141 tttttgacgg cggcagctgc gggaatgtgg tttgcggatg cacaagggct gacatcgcca
     7201 tggtttatcg tgctggtgta cttattccag agcttaggtg aactgtttat tagcgccctt
     7261 ggcctggcga tgattgctgc cctggtgccg cagcatttga tgggctttat tctcgggatg
     7321 tggttcctga cgcaggctgc cgcgttcttg ctgggcggct atgtggcaac atttaccgcg
     7381 gtgccggaca acattaccga cccgcttgag acgttgcccg tctataccaa cgtgtttggt
     7441 aagattggtc tggtcacgct gggcgttgca gtagtgatgc tgttgatggt gccgtggctg
     7501 aaacgcatga ttgcgacgcc ggaaagccat taattattct tgcagaaagc agggtagcgt
     7561 tatcgctacc ctgttttagt tttacactgg cgtacctgca tagatatcaa agcgatgccc
     7621 tttagtgact accgcgtttg gcgtggcgac attcgccagc ggtggcgcgt agtccgggcg
     7681 ctttaccacc acgcgtttgg tcgccagcaa gcgtgcaggc tccagtaatc catcggcatc
     7741 aagatccggt cccaccagcg actgaaagac acgcatctct tttttcacta gcgcgctttt
     7801 ctgcttatgc gggaacatcg ggtcgagata aaccacctgc gggcgcgggg taatatcagt
     7861 cagcgccgtc aggctggagg cgtgaattaa ctgcaaccgc tcctgcaacc agccgccgat
     7921 ttccgcatcc gcataaccac gcgccaggcc gtcgtcgagc aaggcggcaa cgactggatt
     7981 acgctccagc atccgcacgc ggcaaccgac tgaagccagc acaaaggcat cgcgccccag
     8041 ccctgcggtg gcgtccacca catccggcaa ataatcgcct ttaatgccca ccgctttcgc
     8101 caccgcctca ccgcgaccac cgccgaattt acgtcggtgc gccatcgctc cgccaacaaa
     8161 atcaacaaag atgccgccaa gttttggctc atcacgcttg cgcaattcca gatgttccgg
     8221 cgttaacacc agcgccatca ggttgtcttc atcgtgctcc agcccccagc gggccgccag
     8281 aacagataag gcaccgtctc cggtgcctgt ttcatcaatt aagcagattt tcactgaatg
     8341 atcagccctt aatgccgtaa tgctccagca tcgcatccag ctgcggttca cgaccacgga
     8401 agcgtttgaa cagatccatc ggctcttctg aaccgccacg gctcagaatg ttgtcgagga
     8461 acgactgccc ggtttcacgg ttgaaaatgc cctcttcctc aaagcgcgag aaagcatctg
     8521 ccgccagtac gtcagcccac aggtagctgt agtaacctgc ggcataacca ccggcgaaaa
     8581 tatggctgaa agcgtgcggg aaacggcccc aggatggaga cggtactacg gcaaccagtt
     8641 tcttgatttc tgccagagtt tcgaggattt tcgctccctg atccgggcgg aactcggcat
     8701 ggaggcggaa atcgaacagg ccgaactcca gctggcgcag aataaacagc gccgcctggt
     8761 agttcttcgc cgccagcatt ttatccagca actctttcgg cagcggttcg ccggtttcgt
     8821 agtgaccaga gataaacgcc agcgcctcag gctcccagca ccagttttcc ataaactgac
     8881 tcggcagttc gaccgcatcc cacggcaccc cactgatccc ggatacaccg gcggtttcga
     8941 tgcgggtcag catatgatgc agaccatgac cgaactcgtg gaacagggtg atcacttcgt
     9001 catgggtaaa cagcgccggt ttaccattta ccgggcggtt aaagttacag gtcagatacg
     9061 cgaccggttt ttgcagagaa ccgtcagctt tgcgcatctg gcctacgcag tcatccatcc
     9121 acgccccgcc gcgcttgttt tcacgggcat acaggtcgag gtagaagcta ccgcgcagtt
     9181 cgttgttctc gtcatacagt tcgaagaaac gtacatccgg gtgccagaca tcaacatctt
     9241 tacgctcttt agcggtgatg ccgtaaatac gtttcaccac ttcaaacaaa ccgttaaccg
     9301 ctttgttttc cgggaagtac ggacgcagct gttcgtcact gatgctgtag aggtgctgtt
     9361 tttgtttttc gctgtagtac gcgatatccc acggctgcaa ctcatcgacg ccaaattcgg
     9421 ctttggcaaa ggcgcgcagt tgtgccagct ctttttcgcc ttgcggacgc gcgcgttttg
     9481 ccagatcggt taagaaatcc agcacctgct gcgggttttc tgccatttta gtggcaagtg
     9541 atttgaaggc gtagttttca aagcccagca gttgcgccag ttcgtgacgc agcgcgagga
     9601 tctcttccat caccttgctg ttgtcccact taccggcgtt cgggccttga tcggaggcgc
     9661 gggtgctgta agcgcgatac atctcttcac gcagagcctg gttgtcgcag taggtcatta
     9721 ccggcaggta gcttgggata tccagcgtca gcaggtagcc ttcaagttct ttcgcttcag
     9781 cctgggcttt tgccgcagcc agcgcgcttt ctggcatccc cgccagttcc gcttcgtcgg
     9841 taacgagttt ggtccagccc attgtcgcat cgaggacgtt gttgctgtac tggttgccca
     9901 gttcagaaag acgggtagca atttcgccgt aacgctgctg tttctctttc ggcagaccta
     9961 tgccagagag ttcaaaatca cgcagggcgt tatcaaccgc tttcttctgc gccgtgttca
    10021 gcgtggcgta atgatcgcca tcgcgcaggt cgcggtacgc tttatacagc ccttcatgtt
    10081 gccctaccca ggtgctgtat tccgacagca gcggcagggt ttgctcgtag gcttcacgca
    10141 gttccgggct atttttcacc gagttcaggt ggctgaccgg ggagaagata cgccccaaca
    10201 catcgtccac ttccgccaac ggctggcaga gattttccca ggtgtacggt gccccttgcg
    10261 ctactacgcg ctccacattt tcgcggcaat cgttcagcgc ctgagtcacg gctggaacga
    10321 catgttccgg gagaatttta gaaaacggag gcaattcaaa gggagtcagt aacggattcg
    10381 tcattcgcgc agtcctggtt aaagaggtta aggaagcgct caacaggcgc tttacataat
    10441 gtgtgtagca tggggttaag tgtagtgaat ttcaatgaga aacgttacgc tttcgcggcg
    10501 gcggcctctt ttcggtatac tgtcctgata cgcttttgtg cgccccgaat acgggccgat
    10561 ttttacttac cggaacacct ttacccatgc tcagttatcg ccacagcttt cacgctggca
    10621 accacgccga cgtccttaaa cataccgttc agagcctgat catcgagtcg ctgaaagaga
    10681 aagataaacc gtttctctat ctcgacaccc acgcaggggc cgggcgttat cagttaggca
    10741 gcgaacatgc cgagcgtacc ggcgaatatc tcgaaggcat cgcccgtatc tggcagcagg
    10801 acgatttgcc cgcagaactg gaggcgtaca tcaatgtggt aaaacacttc aaccgtagcg
    10861 gtcagttgcg ttactacccc ggttcgccgt tgattgctcg ccagctactg cgtgaacagg
    10921 acagcctgca actgaccgaa ctgcacccga gcgattaccc gttgttgcgt tctgaatttc
    10981 agaaagatag ccgtgcgcgt gtcgaaaaag ccgacggttt ccagcagctt aaggccaaac
    11041 tgccgccggt ttcccgccgt ggtttaatcc ttatcgaccc gccgtatgaa atgaaaactg
    11101 actatcaagc ggtggtcagc gggatagcag aaggttacaa acgtttcgcc actggtactt
    11161 acgcactgtg gtatccggtg gtgctgcgtc agcaaattaa gcgcatgatc cacgatctgg
    11221 aagcgaccgg tattcgcaaa attctgcaaa ttgaactggc ggtactgcca gacagcgatc
    11281 gccgtggcat gaccgcttcc ggcatgattg tgattaaccc gccgtggaaa ctggaacaac
    11341 agatgaataa cgtgctgccg tggctgcaca gcaaactggt tccggcaggc accgggcacg
    11401 ccaccgtaag ctggatcgtg ccggagtaat tgcagccatt gctggcacct attacgtctc
    11461 gcgctacaat cgcggtaatc aacgataagg acactttgtc atgactaaac actatgatta
    11521 catcgccatc ggcggcggca gcggcggtat cgcctccatc aaccgcgcgg ctatgtacgg
    11581 ccagaaatgt gcgctgattg aagccaaaga gctgggcggc acctgcgtaa atgttggctg
    11641 tgtgccgaaa aaagtgatgt ggcacgcggc gcaaatccgt gaagcgatcc atatgtacgg
    11701 cccggattat ggttttgata ccactatcaa taaattcaac tgggaaacgt tgatcgccag
    11761 ccgtaccgcc tatatcgacc gtattcatac ttcctatgaa aacgtgctcg gtaaaaataa
    11821 cgttgatgta atcaaaggct ttgcccgctt cgttgatgcc aaaacgctgg aggtaaacgg
    11881 cgaaaccatc acggccgatc atattctgat cgccacaggc ggtcgtccga gccacccgga
    11941 tattccgggc gtggaatacg gtattgattc tgatggcttc ttcgcccttc ctgctttgcc
    12001 agagcgcgtg gcggttgttg gcgcgggtta catcgccgtt gagctggcgg gcgtgattaa
    12061 cggcctcggc gcgaaaacgc atctgtttgt gcgtaaacat gcgccgctgc gcagcttcga
    12121 cccgatgatt tccgaaacgc tggtcgaagt gatgaacgcc gaaggcccgc agctgcacac
    12181 caacgccatc ccgaaagcgg tagtgaaaaa tgccgatggt agcctgacgc tggagctgga
    12241 agatggtcgc agtgaaacgg tggattgcct gatttgggcg attggtcgcg agcctgccaa
    12301 tgacaacatc aacctggaag ccgctggcgt taaaactaac gaaaaaggct atatcgtcgt
    12361 cgataaatat caaaacacca atattgaagg tatttacgcg gtgggcgata acacgggtgc
    12421 agtggagctg acaccggtgg cagttgcagc gggtcgccgt ctctctgaac gcctgtttaa
    12481 taacaagccg gatgagcatc tggattacag caacattccg accgtggtct tcagccatcc
    12541 gccgattggt actgttggtt taacggaacc gcaggcgcgc gagcagtatg gcgacgatca
    12601 ggtgaaagtg tataaatcct ctttcaccgc gatgtatacc gccgtcacca ctcaccgcca
    12661 gccgtgccgc atgaagctgg tgtgcgttgg atcggaagag aagattgtcg gtattcacgg
    12721 cattggcttt ggtatggacg aaatgttgca gggcttcgcg gtggcgctga agatgggggc
    12781 aaccaaaaaa gacttcgaca ataccgtcgc cattcaccca acggcggcag aagagttcgt
    12841 gacaatgcgt taaatgttaa agggctaaga gtagtgtgct cttagccctt aattacgttt
    12901 ccgctatcag ttcagaagct gaagcagaaa gcggatcagt tccagcagcg caattaacgc
    12961 ccctagaacg atgattgctt tatcaatcac ccgttttctc catgcgatgg agtgagaatg
    13021 catccgctta ctcatccact gcctgtcacg gcgcatgtct cattgttaga taagaactct
    13081 ctcactccgg ccagagcatc agttaacggc accacccgta cttctgacca ggactttgaa
    13141 agcgtttatg cgcattgcca gagtgaaaat gcctcagagc taactggata atcatacagt
    13201 acatgcaggt tataaaacca gcacgtcctt gcaatagttt cagtatggta ttagcattga
    13261 tgcgttagat gatggctatc tcactccagt cagagccacc aactcagggc tggaaagtaa
    13321 aaaaccgacg caaagtcggt ttttttacat ccggattcgg acaaggctta atatgacgat
    13381 gacccagtga aagtatataa atcgtcactg cgatatatac cgaagtgctc cctccgccag
    13441 ctgaagaaat cgctaattct tgcaatgtta gccactggct aatagtattg agctgttaga
    13501 taagaactct ctcactccag ccagagccac caactcaggg ctggaaagta aaaaaccgac
    13561 gcaaagtcgg tttttttacg tcctgattca gacctccttt caaatgaata gccaactcaa
    13621 aattcacacc tattaccttc ctctgcactt acacattcgt taagtcatat atgtttttga
    13681 cttatccgct tcgaagagag acactacctg caacaatcag gagcgcaata tgtcatttct
    13741 gttacccatc caattgttca aaattcttgc tgatgaaacc cgtctgggca tcgttttact
    13801 gctcagcgaa ctgggagagt tatgcgtctg cgatctctgc actgctctcg accagtcgca
    13861 gcccaagatc tcccgccacc tggcattgct gcgtgaaagc gggctattgc tggaccgcaa
    13921 gcaaggtaag tgggttcatt accgcttatc accgcatatt ccagcatggg cggcgaaaat
    13981 tattgatgag gcctggcgat gtgaacagga aaaggttcag gcgattgtcc gcaacctggc
    14041 tcgacaaaac tgttccgggg acagtaagaa catttgcagt taaaaattta gctaaacaca
    14101 tatgaatttt cagatgtgtt ttatccggga ggcattatgt tactggcagg cgctatcttt
    14161 gtcctgacca tcgtattggt tatctggcag ccgaaaggtt taggcatcgg ctggagtgca
    14221 acgctcggcg cagtactggc gttagttacg ggcgtggtcc atccgggtga tattccggtg
    14281 gtgtggaata tcgtctggaa cgcgacggct gcgtttatcg ccgtcattat catcagcctg
    14341 ctgctggatg agtccggctt ttttgaatgg gcggcgctgc acgtctcacg ctggggtaat
    14401 ggtcgtggtc gcttgctgtt tacctggatt gtcctgctcg gtgctgccgt tgccgccctg
    14461 tttgccaatg atggcgcggc gcttattttg acaccgattg tcatcgccat gctgctggct
    14521 ttagggttca gtaaaggcac tacgctggcg ttcgtgatgg cggccggatt cattgccgat
    14581 accgccagcc tgccgcttat tgtctccaac ctggtgaata tcgtttccgc tgatttcttt
    14641 ggcctcggct ttcgcgaata cgcctcggtg atggtgccgg tggatatcgc cgcgattgtt
    14701 gccacgctgg tgatgttaca tctctatttt cgcaaagata ttccgcagaa ctacgatatg
    14761 gcgctgctga aatctcccgc agaagcgatc aaagatcctg ctacgttcaa agctggctgg
    14821 gttgttttac tgcttctgct ggtgggattt ttcgtcctgg aaccgctcgg cattccggtg
    14881 agcgccattg cagctgtggg cgcgctgata ttatttgtcg ttgctaaacg cggtcatgcg
    14941 attaatacgg gtaaagtcct gcgcggtgcc ccctggcaga ttgtcatctt ctcgctcggc
    15001 atgtatctgg tggtttatgg cctgcgcaat gccggattaa cggaatatct ttctggcgta
    15061 ctcaacgtgc tggcggataa cggcctgtgg gccgcgacgc tcggcaccgg attcctcacc
    15121 gccttcctct cttctattat gaacaatatg ccgacggtac tggttggcgc gttgtccatt
    15181 gatggcagca cggcatctgg cgttatcaaa gaagcgatgg tttatgccaa tgtgattggc
    15241 tgcgatttgg gaccgaaaat taccccaatt ggtagcctgg ctacgctact ctggctgcac
    15301 gtactttcgc agaagaatat gactatcagc tggggatatt acttccgtac agggattatc
    15361 atgaccctgc ctgtgctgtt tgtgacgctg gctgcgctgg cgctacgtct ctctttcact
    15421 ttgtaatgag atactgatat gagcaacatt accatttatc acaacccggc ctgcggcacg
    15481 tcgcgtaata cgctggagat gatccgcaac agcggcacag aaccgaccat tatccattat
    15541 ctggaaactc cgccaacgcg cgatgaactg gtcaaactca ttgccgatat ggggatttcc
    15601 gtacgcgcgc tgctgcgtaa aaacgtcgaa ccgtatgagg agctgggcct tgcggaagat
    15661 aaatttactg acgatcggtt aatcgacttt atgcttcagc acccgattct gattaatcgc
    15721 ccgattgtgg tgacgccgct gggaactcgc ctgtgccgcc cttcagaagt ggtgctggaa
    15781 attctgccag atgcgcaaaa aggcgcattc tccaaggaag atggcgagaa agtggttgat
    15841 gaagcgggta agcgcctgaa ataaagcggc gatatccact gccgatagtt ggtaaagtgc
    15901 gctttgttta tgtcggatgc gacgctaacg cgtcttatcc gacctacaaa accgtgcaaa
    15961 ttcaatatat ggcatgaaat atgtagagcg tagcgcatca ggcaattttg ctatttacgc
    16021 agcactcctc gcgtaagaat tcaattatcg cttccagcat tgcgtattgc gaaacacaca
    16081 tcagcgtccg actctcacgg ttttgtttga ccagtcctac cgataccagt gctgatatat
    16141 ggtggctgag tgtggagcca ggtatcccaa gctgcttttg caactcaccg accggcaacc
    16201 cttgttcccc ggctttgaca agatgtttaa atatgaacaa acgggtcggg tgacctagct
    16261 ctttcagcgc tttagcgact tcttccaact gcatatgaca cccctctgtc tggttgatat
    16321 ttcgataata ccagaaatat cttgatctga atttcatttc gattattctg gaaatataga
    16381 tttatccaat gaggttacgt atgagtagtt ggcttgcgat gctgcaagat gccgcagaga
    16441 tgtttgtgtt tctcgccgtc gagctttcgt tgctgtttat agtgattagt gccggtgtca
    16501 gcctgataag acaaaaggtg ccagaccata aaatccagca gatgatgggg gccagaaaag
    16561 ggagaggcta tctccaggct gctctgttgg gagccgttac cccgttctgt agttgctcga
    16621 caatacccat gttacgtgga ttgttgtcag cgaaagccgg gtttggtccg actctcactt
    16681 ttttatttgt ttcaccatta cttaatccca ttatcgtcgg actaatgtgg atgacctttg
    16741 gttggaaagt caccttgttg tacgcgatta tcgcagccgg cgtctccgtt cttgccagta
    16801 ttatcctgga ttctctgggg tttgaacgtc atatcattgc cagtaaaagc tcatcagcaa
    16861 attgttgtgc tccagcaaaa acttcgccgg gaacgacata tacgcccata aaagtgagtt
    16921 gttgtagtcc agcggctaaa gccattgaga accccgtagt tacctgttgc agtaccaaag
    16981 ctgtagtaag tattaatccc ataaaactag ccaccaaaga tgcgttgcaa cagtttaaag
    17041 atgtactgcc atatcttttg ttaggagtat taataggatc ttttatttat ggctttattc
    17101 cctcagcgtg gattgccgct catgcaggag cagataatcc cttcgccatc ccattaagcg
    17161 ccgttgtcgg tattccgctg tatatccggg ctgaggcagt tatccctctg gcatctgttc
    17221 tgatgacaaa agggatgggt ctgggagcat taatggcatt aatcatcggc agtgccggcg
    17281 caagcctgac ggaagtgata ttacttaaat caatgttcag aatgccgatg atagctgcat
    17341 tcctgacggt tatattaggt atggctatct tgatgggcta tttgactcaa ttgctatttt
    17401 aaatagatgc cagcagtaaa ctataagatg catagttagt gccatatcct tctaagtaga
    17461 aaatcataga caggataatc cttaaaaatc acaaatgatt taactaatgc attgagaatg
    17521 atgacggctc ggtcaacaag tcatcaacag acaataacca cgtaatcatg cttctctgac
    17581 attttatcgg agaagc
//
LOCUS       DAZFAH010000092        17275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFAH010000092 DAZFAH010000000
VERSION     DAZFAH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.092"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..1740)
                     /locus_tag="ACHJWB_002823"
                     /pseudo
     CDS             complement(97..1740)
                     /locus_tag="ACHJWB_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
     gene            complement(2092..2256)
                     /gene="yjiS"
                     /locus_tag="ACHJWB_002824"
     CDS             complement(2092..2256)
                     /gene="yjiS"
                     /locus_tag="ACHJWB_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4314425.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLSREDVE"
     gene            2433..3845
                     /gene="yjiR"
                     /locus_tag="ACHJWB_002825"
     CDS             2433..3845
                     /gene="yjiR"
                     /locus_tag="ACHJWB_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4314426.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(4087..5043)
                     /locus_tag="ACHJWB_002826"
     CDS             complement(4087..5043)
                     /locus_tag="ACHJWB_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO4314427.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGRRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKLDPLIEF"
     gene            5510..6742
                     /gene="mdtM"
                     /locus_tag="ACHJWB_002827"
     CDS             5510..6742
                     /gene="mdtM"
                     /locus_tag="ACHJWB_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4314428.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            6783..8063
                     /gene="yjiN"
                     /locus_tag="ACHJWB_002828"
     CDS             6783..8063
                     /gene="yjiN"
                     /locus_tag="ACHJWB_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4314429.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDT
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            8179..9330
                     /gene="yjiM"
                     /locus_tag="ACHJWB_002829"
     CDS             8179..9330
                     /gene="yjiM"
                     /locus_tag="ACHJWB_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4314430.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLIGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9340..10107
                     /gene="yjiL"
                     /locus_tag="ACHJWB_002830"
     CDS             9340..10107
                     /gene="yjiL"
                     /locus_tag="ACHJWB_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4314431.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            10104..10361
                     /gene="ytiA"
                     /locus_tag="ACHJWB_002831"
     CDS             10104..10361
                     /gene="ytiA"
                     /locus_tag="ACHJWB_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4314432.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            10426..11286
                     /gene="yjiK"
                     /locus_tag="ACHJWB_002832"
     CDS             10426..11286
                     /gene="yjiK"
                     /locus_tag="ACHJWB_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4314433.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            11354..12532
                     /gene="yjiJ"
                     /locus_tag="ACHJWB_002833"
     CDS             11354..12532
                     /gene="yjiJ"
                     /locus_tag="ACHJWB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4314434.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(12545..13099)
                     /gene="kptA"
                     /locus_tag="ACHJWB_002834"
     CDS             complement(12545..13099)
                     /gene="kptA"
                     /locus_tag="ACHJWB_002834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO4314435.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            13349..14032
                     /gene="yjiH"
                     /locus_tag="ACHJWB_002835"
     CDS             13349..14032
                     /gene="yjiH"
                     /locus_tag="ACHJWB_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4314436.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14029..14490
                     /gene="yjiG"
                     /locus_tag="ACHJWB_002836"
     CDS             14029..14490
                     /gene="yjiG"
                     /locus_tag="ACHJWB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4314437.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            14503..15675
                     /gene="iadA"
                     /locus_tag="ACHJWB_002837"
     CDS             14503..15675
                     /gene="iadA"
                     /locus_tag="ACHJWB_002837"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4314438.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            15740..16651
                     /gene="hypT"
                     /locus_tag="ACHJWB_002838"
                     /gene_synonym="qseD"
     CDS             15740..16651
                     /gene="hypT"
                     /locus_tag="ACHJWB_002838"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO4314439.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(16644..17036)
                     /gene="iraD"
                     /locus_tag="ACHJWB_002839"
     CDS             complement(16644..17036)
                     /gene="iraD"
                     /locus_tag="ACHJWB_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4314440.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17033..17116)
                     /gene="idlP"
                     /locus_tag="ACHJWB_002840"
     CDS             complement(17033..17116)
                     /gene="idlP"
                     /locus_tag="ACHJWB_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4314441.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3956 a         4398 c         4378 g         4543 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggcgtcat aacatgcttg acaacttaat aggggcaccc cacaaaaagg caaacagatc
      121 cgaagcggga taatcttttt ggggagaatt gttccagtta gcaatatatc ccgacagggg
      181 gttatacacc ttagggttca tttcaaaagg caatagccct ttccagtccc attttcccgt
      241 accaggaacg ggtaatcgcg gatcatggcc tgattgacga tctggataag caccagtatg
      301 aacataacca atattgccgt ttacatcagc atagtaccag ttgatggtca gtgcttgttt
      361 cgctgcctgc tgtgtccact cctgccaatt tttggccttc atctgatgag tccaggccag
      421 caaagacgcc acctctttac catcccatgc gcgggattta gcgtaagccg tttgtgtcgt
      481 ctggtcagtt tggagaatgt tgccatgcac cgtacgccag acagtaaagg tctctgcctg
      541 accatttttc accgtaatgg tttcctcacg gcttaacatt ttcacccact taccattatg
      601 caagtagtag cctggtttct ctgccgacag ccgttcagca aaaatatcga catcatcgcc
      661 gaaacctgcc gttgatcccc aggaaatcac accattatga ccaaaaacca gcccaggata
      721 ggcaaatggt gtattgccag tgacatcata accagcaccg tgcagaccaa taccataagt
      781 atacgcaggc gcataccagc caaactacgg accatttacc atgattgctt tcgcatcctg
      841 ggctttgctt ttgccgatca cccacatatt gctggtcgtt ggataccccg ccagaccatt
      901 ggcaccaccc tgtgcaaatt gtgcagcaat agtttcccgg ttcttccctg ctgttaacgc
      961 cagcagtgcg ccatccgccc cttttgctgg tcggtcaagc attggtgcag gtaaatcgta
     1021 gcgtggcaac agagctgctg tttgcgagtt ttgctgatta aatttaagtg ggtagctact
     1081 ctcttgtacg gcaatagtgg ttggcgctga tgggtttacc agccatttca actgattaaa
     1141 taccgccatg ccttgtgata caccatattt atcttttaaa gccgttagca gtgccagatt
     1201 atcaatttcg ctagtgctat cagagaagcg gtttgccatg gtgcccacaa atatcatcgc
     1261 gacatcaaac ggttcccagc gcttaggagt aaagccaaat gtattaaact gttttggtaa
     1321 gagcgtctct ggattggtat ttaccttatc aatccaggca ttcattccat cagcgtagcc
     1381 ttgcagaatg gacatatcct ctggggaaag ggcagcaatt tgcgcccgga tagcatccgg
     1441 ccagtagtta cgacggatat ctttatcaaa tttcacaaaa tctttgccaa gcacttccgc
     1501 gacagtccct tgagtactgc gacgtgccat ttccatctga aaaaggcgat cttgtgctac
     1561 tacatagcca tagccataaa ataggtgcca tgtatcattg gcataaatat gcggcatgcc
     1621 gtattcatcg cgaacaatct ttatctcact tgacgactgc tcagccagtg caggtaagct
     1681 ccaataatac atcagggaag cagtaacaca gttcacgatc atacgatttc tatttttcat
     1741 tgtatcctct ggcaggtgta taattagcga attgatacta gcaacgaagc ttcggttatc
     1801 tatttgcaga gaattgtttc attatgaact gtgagcgcga taactaattt ttgatacaaa
     1861 aatttcatta atgtgatcta aaacgaatcg ctttattcac cgctgatgat tttgttctat
     1921 tcctttttgt gacagatccc ccggacgcat agatgatgac ccccagagag acagctcagg
     1981 tcattatctg tatgtaaatc agaaaaatat gttgtaaacg acttttatgg ttggattttg
     2041 tagcaatttt gaatgcagac aggcagcatt tgccaacacg ccgcccatag ttcactccac
     2101 atcctccctg cttaacccaa tatccctcaa ccgttcatca ctcatctgct gtaacactcg
     2161 tcgggtctgc atttgccgcc gccaacgcct caccgcatgc cagagttgca caagtctgat
     2221 aaacggcgtt ttggctttgt tttcgtgaaa ttccatctgc tgcctcctca tctttcagag
     2281 gcttcatcat ccgccttctg gcagttcaca atacagatgc aaatattagt tttatttaac
     2341 atacagacag gttaaaacag catctgcatc cggtttttta gcctcatctg taccggtttt
     2401 tctttctgta tgggccaaat aaggaataca gcatgacgcg ttatcaacat ctggcgactc
     2461 tacttgccga gcggattgag caagggctgt atcgtcacgg ggagaaattg ccgtcggtgc
     2521 gcagcttaag tcaggagcac ggcgtcagca tcagcaccgt gcaacaggcg tatcagacgc
     2581 tggaaacgat gaagctcatc actccgcagc cgcgttcggg ttattttgtc gcacaacgta
     2641 aagcccagcc gcctgtaccg ccgatgacgc gtccggtgca gcgcccggtg gaaattaccc
     2701 agtgggatca ggtgctggat atgctggtgg cgcatagcga cagttccatt gttccgttaa
     2761 gcaaaagcac gccggatgtc gaaacgccca gcctgaaacc gctctggcgg gagctaagcc
     2821 gggtggtgca gcataatctg caaaccgtgc tcggttatga cttgttagcc ggtcagcggg
     2881 tattgcgaga gcagattgcc cgcctgatgc tcgacagcgg ctcggtggtc accgccgatg
     2941 acatcatcat caccagcggc tgccataact cgatgtcgct ggcgttaatg gcggtgtgta
     3001 aaccgggcga tattgtcgcg gtcgaatctc cctgttatta cggttcgatg cagatgctgc
     3061 gaggcatggg cgtgaaagtg attgaaatcc caaccgatcc agaaactggc atcagcgttg
     3121 aagcactgga actggcgctg gaacagtggc cgattaaagg catcattctg gtgccaaact
     3181 gtaataatcc gctgggattt attatgccgg acgcacgcaa acgggccgtt ctctctctcg
     3241 ctcagcgtca tgatattgtg atttttgaag atgatgtcta cggcgaactg gcgacggagt
     3301 atccgcgccc gcggaccatt cattcctggg atatcgacgg gcgagtgctg ttgtgcagct
     3361 cgttcagtaa aagcattgca cctggcctgc gcgtgggttg ggtcgcaccg gggcgctatc
     3421 acgataaact gctgcatatg aaatacgcca tcagcagctt taatgtaccg tccacgcaaa
     3481 tggcggcggc aacgtttgtg ctggaaggtc actatcatcg ccatatccgg cggatgcggc
     3541 agacttatca gcgcaatctg gcgctttata cctgctggat acgggaatat tttccctgcg
     3601 aaatctgtat tacgcgcccg aaaggcggat ttttactgtg ggtagaattg cctgaacagg
     3661 tcgatatggt ctgcgtcgcg cggcagctgt accgcatgaa aatccaggtg gcggcaggct
     3721 cgattttctc agcgtccggc aaataccgta attgtctgcg catcaactgc gctttaccgc
     3781 tcagcgaaac ctatcgcgaa gcactaaagc aaattggcga ggccgtgtat caggcaatgg
     3841 aatgaattcg aaatagtggc gaagcacctg atttactttc acgaatcgcc tgtaactcgt
     3901 tttatcttta tagcgacatc gccacgttcc ggcatagctt aggcaaagtg tgctttgttc
     3961 atgccggatg cggcgtgaac gccttatccg gcctacaaaa ccagcaaatt caatagattg
     4021 cagaggtaat gtaggtctga taagcgtagc acatcaggca gctttgcgtt tgtcatcagc
     4081 ctctgattag aattcgatca atggatcgag cttgctttca ggcaagggct gatctaatgc
     4141 ctgcatttgc tctgccgaaa gtccggtata tcgttgtatc cattcaggat cggccccatt
     4201 ctgcaacaac aatcggagga ggctccgttc cccttctact ttcccttctt tcctccccct
     4261 ctcccttccc aacagccatc catcattatg aattcgctct gcaattgtca ttattcgctc
     4321 cctgtggtgt ggcattcgcc tggtaagttc gctgataaac gccttaaaac gcgcttcatc
     4381 gccagtcagt aaaatgtaat ttaacagcgc agttatctgg ctgtcattag cacactctgt
     4441 aaccagtaat actaccagtt ggtcgataag ccccatcaga tcgcgctggc gaatatgctt
     4501 ttggatcaac tccaacaggg cgactctgcg atgctgcaca atctcgtcgt ctggcacgac
     4561 agtgacatcc accagcggga acgctgcgct ataaagtttc cgtgcggtag tcgggtcggc
     4621 aaattcgtcc agccagcaca gggaccaggg ataaggacta cgtctaccgt gataaaatag
     4681 catcgggatg accagcggta gcggccgacg tttatcatgc tcgatatggc gctgcatcac
     4741 cgccatggaa tagcgcatca ggcgaaaggc catatgaatg tcctcgcggc tctgatgttc
     4801 aatcaccaca tagatataac catcgccttc gcgggtcttt accgaccaca gaatatcgga
     4861 atgtagcgcc cgcaattttt catcgacaaa gctggcggat tccagtttta agctgtcgag
     4921 atcgcacagt tcacgtaaat ctttaggtaa gtggatctcc ataaaatccc gcgcggtgtc
     4981 agggtgcgtg agaaaggatt taaataatgc gtcatgcggc gtgctggtcg agaagtttgt
     5041 catggcgatc cgtcacctgt agataagatg gcccgacttt agcggctaac aaaaccattc
     5101 tcacccggca atttcccgtt ccccgaggcg ctttccaggc tatttttatt cttctgcaaa
     5161 cgatacattt tttcggaaac aggctcgcgt tttacatttc ctgtacccgg cagatattcc
     5221 cctttccacc gcgctctttt gattaaccgc aaattttaat tacacttaag gtgtatattt
     5281 tctatgcaac ccatcaatta aagaggtgta atgtgctgat gactatttgt aatcgttata
     5341 cattctgacc tggagtcaga aggtatttct ctgtctgtgt gttcacaggc agtgtggttg
     5401 attacatgaa attcagtaca tttgtccttc tcgcctgcct ttcgtcatta ccgacggtat
     5461 tgaatttcgt tttccccgtt ggggttctcc ggacaaggag ttgtttgtta tgccacgttt
     5521 ttttgcccgc catgccgcca cgctgttttt cccgatggcg ttgattttgt atgactttgc
     5581 cgcgtacctg tcgacggatc tgatccagcc ggggatcatt aatgtggtcc gtgattttaa
     5641 tgccgatgtc agtctggccc cggcagccgt cagtctctat ctcgcgggcg gtatggcgtt
     5701 acagtggctg ctggggccgc tttccgacag gattggccgc aggccggtgc tgattaccgg
     5761 ggcgctaata tttacccttg cctgcgctgc gacaatgttc accacgtcta tgacacagtt
     5821 tcttatcgcg cgtgcaattc agggcaccag tatctgtttt attgccaccg ttggttatgt
     5881 cacggtgcag gaggcgttcg gacagacaaa agggattaag ttgatggcga ttatcacctc
     5941 catcgtactg attgcgccga ttattggccc gctttccggc gcagctctga tgcactttgt
     6001 acactggaaa gtcctttttg ccatcattgc ggttatgggt tttatctcat ttgttggctt
     6061 actgctggcg atgccagaga cggtgaagcg cggcgcggtt ccatttagcg ccaaaagcgt
     6121 cttgcgcgat tttcgtaatg tcttttgcaa tcggctgttc ctctttggcg cagcaaccat
     6181 ctctttaagc tatatcccaa tgatgagttg ggtggctgtc tcgccggtga tcctgatcga
     6241 cgcgggcggc ttaacaactt cgcagttcgc ctggacacag gttccggtgt tcggcgcggt
     6301 gattgtcgcg aatgccatcg tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat
     6361 ctggcgtgct gtacccattc aactggtcgg cctcgcgctg ttgattatcg gcaatctgct
     6421 gtcaccgcac gtctggctgt ggtcggtgct gggcaccagt ttgtatgctt tcgggattgg
     6481 tttgatcttc ccgaccttat tccgctttac gcttttttcc aataacttac cgaaagggac
     6541 cgtctccgca tcgctgaata tggtgatcct gatggtgatg tctgtctcgg tcgaaatcgg
     6601 ccgctggctg tggtttaacg gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg
     6661 cgttatcgtc gttttcaccc tggcaggatt gcttaatcgc gtgcgccaac atcaggctgc
     6721 cgagctggcg gaggagcggt gatttttgcg cgatcctgcc gtcaggctct attcttaacg
     6781 ttatgaataa actcattgaa ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta
     6841 tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc caatttttgg gtgagcggcg
     6901 tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg
     6961 cgctgtttcg ccgcgtgccg attccgatca tttctcgtca tacggcgatt atcccgcgta
     7021 ataaagaccg gattggcgaa aatctcggcc agttcgtgca ggaaaaattt ctcgataccc
     7081 agtcgctggt ggcattgatt cgacgtcacg aaccggcgtt gttgattggc aactggttta
     7141 gtcagccgga gaacgcccgc cgcgttggtc agcatctgtt gcagatcatg agcggttttc
     7201 ttgaactgac cgatgatgca cgtattcagc gcctgcttaa gcgcgcggtc catcgggcga
     7261 ttgataaggt cgatctttcc ggaaccagtg cgttgatgct ggagagtatg accaaaaacg
     7321 atcgtcatca ggtgctgctc gatacgctga tcgcacagtt gatcgccctt ctccagcgcg
     7381 ataaatcgcg caagtttatt gcccagcaga ttgttcgctg gctggagagt gagcatccac
     7441 tgaaagccaa aattctcccc accgaatggt tgggcgaaca tagcgcggag ttggtttctg
     7501 acgcggtgaa ttctttgctt gatgatatca gccgtgatcg tgcgcatcag atccgtcatg
     7561 cgtttgatcg cgccactttt gccctgatcg acaagttgaa aaacgatacg gaaatggcag
     7621 cgcgagccga tgccgtaaaa agttatctga aagaagatga agcttttaac cgctatctca
     7681 gtgaattgtg gggggattta cgggaatggc tgaaagcgga tatcaacagt gaagattctc
     7741 gtgtgaaaga acgtatcgcg cgggcgggtc aatggtttgg cgaaacgtta attgccgatg
     7801 atgccttgcg ggcatcgtta aatggtcacc tggaacaagc cgcgcaccgc gtcgcgcctg
     7861 agttttccgc attcctgacg cgccacatca gcgatacagt aaaaagctgg gatgctcggg
     7921 atatgtcgcg gcaaatcgag ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg
     7981 gtacgctggt tggcggttgt attgggctaa ttttatattt gttgtcgcag ctcccggcct
     8041 tgttccccct cagcaatttt tagaaatcca tgaaaaacta agtggctaac cgcgagagag
     8101 atcaaataaa ccacactaaa agaatggtaa taatgtggcc tcttttcatt atctcccgtg
     8161 gtacggggaa ggaaaatcat gtcacttatc accgatctac ccgccatttt cgatcagttc
     8221 tctgaagctc gccagaaagg ctttctcacc gtcatggatc tcaaggagcg cggcattccg
     8281 ctgattggca cttactgcac ctttatgccg caagagatcc cgatggcagc cggtgcggtt
     8341 gtggtttcgc tctgttctac ctctgatgaa accattgaag aagcggagaa agatctgccg
     8401 cgcaacctct gcccgctgat taaaagcagc tacggctttg gcaaaaccga taaatgcccc
     8461 tacttctact tttcggatct ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg
     8521 tatgaataca tggcggagtt taagcccgtt catgtgatgc agttgccgaa cagcgttaag
     8581 gacgatgcct cgcgtgcgtt atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa
     8641 gaacgttttg ggcacgagat tagcgaagat gctctgcgcg atgccattgc gctgaaaaac
     8701 cgcgaacgtc gcgcactggc taatttttat catcttgggc agttaaatcc tccggcgctt
     8761 agcggcagcg acattctgaa agtggtctac ggcgcaacct tccggttcga taaagaggcg
     8821 ttgatcaatg aactggacgc gatgacagcc cgcgttcgtc agcagtggga agaaggccag
     8881 cggctggccc tgcgtccacg cattttaatc accggctgcc cgattggcgg cgcagcagaa
     8941 aaagtggtgc gcgcgattga agagaatggc ggctgggttg tcggttatga aaactgcacc
     9001 ggggcgaaag cgaccgagca atgcgtggca gaaacgggcg atgtctacga cgcactggcg
     9061 gataaatatc tggcgattgg ctgctcctgt gtttcgccga acgatcagcg cctgcaaatg
     9121 ctcagccaga tggtggaaga atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg
     9181 tgccatacct acgcggtgga atcgctggcg attaaacgtc atgtgcgtca gcagcacaac
     9241 attccttata tcgctattga aacagactac tccacctcag atgtcgggca gctcagtacc
     9301 cgtgtcgcgg cctttattga gatgctgtaa ggagtggcag tgacatattc gattggcatt
     9361 gattctggtt ctaccgccac caaagggatc ttactggcag acggcgtgat tacgcgccgt
     9421 ttcctcgttc caacaccctt tcgtccggca acagcaatta ctgaagcctg ggaaactctg
     9481 cgcgaagggt tagagacaac gccgtttctg acgctcaccg gctatggacg gcaactggtg
     9541 gattttgccg ataaacaagt gacggaaatc tcctgtcatg ggctgggcgc acggtttctt
     9601 gcgccagcaa cgcgcgcggt aatcgacatc ggtggtcagg acagcaaagt gattcagctt
     9661 gatgatgacg gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc gggcaccggg
     9721 cgtttcctgg aggtgatctc gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt
     9781 accgaaaatg tcacgccgca cgccatcacg agtatgtgca cagtgtttgc cgaatcagaa
     9841 gtgatcagcc tgcgctcagc gggcgtcgcg ccagaagcga ttctcgccgg agtgattaac
     9901 gcgatggcgc ggaggagtgc caatttcatt gctcgtctct cctgtgaagc gccgattctg
     9961 tttactggtg gcgttagtca ttgccagacg tttgcccgga tgctggaatc tcatctggga
    10021 atgccggtaa atacccatcc tgatgcgcaa tttgctggcg caattggcgc ggcggtaatt
    10081 ggtcaacgag tgaggacacg ccgatgaaag agtttttatt tttatttcac tccacagtcg
    10141 gcgtcataca aacccgcaaa gcgttgcagg cagcgggcat gacttttcgc gtcagcgata
    10201 ttccccgtga tttacgcggc ggctgcgggt tatgcatttg gctgacctgt ccccccggcg
    10261 aggaaataca atgggtgatc cctgggctga ctgagtcaat ttattgccag caggatggtg
    10321 tctggcgctg cattgcacat taccgggttt ctccgcgtta aggattcatt aaggtcgcta
    10381 cgtatacttc tcgatatctt attgatttgc cttaaaggga ttaccgtgac aaaaagtatt
    10441 tcgttaagta aacgcatctc cgttattgtg attctttttg ccattgtggc agtgtgtact
    10501 ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag catcatttca gaattaccac
    10561 gccaccattg acggcaaaga aattgcaggc ataacgaata atatctcttc gctgacctgg
    10621 tcggcacaaa gcaatactct ttttagcact atcaacaaac ctgccgccat tgttgaaatg
    10681 accaccaatg gtgattttat ccgcacaatt cctctggatt ttgttaagga tctcgagacg
    10741 atcgaataca ttggcgataa tcagtttgtc attagcgatg agcgcgatta cgccatttat
    10801 gtgatttcac tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa aatcccgctg
    10861 caagactccc caaccaattg cggttttgaa gggttggctt attctcgcca ggatcacact
    10921 ttctggtttt ttaaagagaa aaatccgatt gaggtctaca aagttaacgg gctgttaagt
    10981 agcaatgaat tacacatcag caaagacgag gcactacaac ggcaatttac tttagatgat
    11041 gtgtccggcg cagaatttaa ccagcaaaaa aatacgcttc tggtgctgtc tcatgaatct
    11101 cgcgcactcc aggaagtaac cctggttggc gaggttatcg gtgaaatatc actaaccaaa
    11161 ggtagtcgag gtttgtcgca taacatcaaa caggccgaag ggattgcgat ggatgcctct
    11221 ggtaatattt atatcgttag cgaaccaaat cgtttttatc gctttacccc taaatcatcg
    11281 cattaacagg ctggcatatt tcaccttttt gttattaact tgttaacaac aaaaatgaca
    11341 tacaaggtta aacatgcctt cgtcctcgca tcccgtagaa cgcttttctt tcagcaccgc
    11401 gcttttcggg atgctggttc tgaccctagg tatgggatta ggtcgcttcc tttatacgcc
    11461 tatgttgccc gtcatgatgg cggaaggggc gttttcattt agccagctct cgtggattgc
    11521 cagcggcaac tatgcggggt atctggctgg cagtctgcta ttttcgtttg gcgcatttca
    11581 ccagccgtcg cgcctacgcc catttctgtt ggtttctgcc ctggcgagcg ggttgttgat
    11641 cctcgcaatg gcatggttgc cgccatttat tctggtgtta ttgattcgct tcctggcggg
    11701 tgtcgccagc gccggtatgc tgatttttgg ttcgacgctg attatgcagc acacgcgcca
    11761 tccttttgtg ctggcagctt tgttttctgg cgttggcatt ggcattgcac tgggcaacga
    11821 atatgttctg gcaggcctgc attttgcctt atcctcgcaa acgttatggc aaggcgccgg
    11881 ggcgctttct ggcatgatgc tgattgcact tacgctttta atgccctcga aaaaacacgc
    11941 catcgcacca atgccattgg caaaaacgga gcaacagata atgagctggt ggttactggc
    12001 tattctgtac ggcctggcgg gatttggtta tatcatcgtc gcgacttacc tgccgctcat
    12061 ggcgaaagat gcaggctcac cattgttaac cgcccatctc tggacgttag tcggcttatc
    12121 aattgtgcca ggctgcttta gctggctatg ggcagcaaaa cgttgggggg ctttgccttg
    12181 cctgacggcg aatttgctgg tgcaggctat cagtgtgctg cttactctcg ccagcgactc
    12241 tcctctcttg cttatcatta gtagtatcgg ttttggcggt acctttatgg gcacgacatc
    12301 tctggtgatg actatcgccc gccaactgag cgttccggga aatctcaatc ttttaggctt
    12361 tgtgacgctc atttacggca ttggacaaat cctcggcccg gcgctgacca gtatgctcgg
    12421 caatggaaca tcggcgcttg ccggcgccac tctctgcggc gcggcggcgt tgtttattgc
    12481 tgcgcttatc agcacagtac aattatttaa gctgcaagtg gtcacttcat gaggagtgtt
    12541 tcccttacca ctcaagaaat tcgaccgcaa ccgttgatgt cagccagact ccgttttccg
    12601 cttgccagaa agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagataaccg
    12661 gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt
    12721 aatgacgctc ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg
    12781 taccgtggta aagaaactgt ggcggtgttt tttcggcaaa ggaaatggcg acctgcgaag
    12841 tcgaatgccc ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt
    12901 tatcactggt agcgaccacg gtatccagca atgcacgggt taaccgcttg cccgcttttt
    12961 gggcacagag tataagctta tcgatatccg cccatccttc acggtcaagt acgataccaa
    13021 tcgcctccgg tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccaactctt
    13081 tttcgttgta ctttgccatc tcgttttcgt ccctggattt tctgttttta tttactgatg
    13141 tcattgtcac tgggtttatg aattttctgc aactcaatcc tgcgcagagc cttttccaca
    13201 cagcataatt tttccgctat gcaaaaaacg catagccaga aacatacttg cattttcccc
    13261 atacccacat ggctgataag gtttttccgc tccaggaatt ttcctggctc aagcaacaca
    13321 caaacacaaa aaaacaatgg ggattgttat gacacaacaa ggggatgctg tagccgggga
    13381 gctggcgaca gaaaaagtgg gcattaaagg gtatctggct ttttttctca ccataatctt
    13441 tttttccggt gttttttccg gaacagacag ctggtggcgc gtttttgatt tttccgttct
    13501 gaacggatcg tttgggcaac tgcctggtgc gaacggagcc accacctcat ttcgcggggc
    13561 gggtggcgcg ggggctaaag atggctttct ctttgcgctg gagctggcac cgtcagtcat
    13621 tctctctctg gggattattt caattaccga tggtcttggt ggattacgcg ccgcgcagca
    13681 gttaatgacg cctgtgctaa aaccgttgtt gggcattcca gggatctgct cgctggcgct
    13741 gatcgctaac ctgcaaaaca ctgatgccgc cgccggtatg accaaagagc tggcgcagga
    13801 aggggaaatt accgaacgcg ataaagtgat tttcgccgca tatcaaacca gcggtagcgc
    13861 catcatcacc aactacttct cttccggcgt cgcggtgttt gcctttctgg gcacgtccgt
    13921 aatcgtacct ttagccgtca tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt
    13981 ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag
    14041 gtccgcaaaa atgtcatgga tatgtttatc gatggtgcgc gccgtggctt tactatcgcc
    14101 acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca tcattcaggc gctgaaaatt
    14161 accggcctgc tcgactgggt ggggcatatc tgtgaacccg taatggcgct gtggggatta
    14221 cccggtgaag cggcaaccgt ccttttagcc gcattaatga gtatgggcgg cgcagtcggt
    14281 gttgccgcca gtctggcgac cgccggcgca ttaaccggtc acgatgtcac cgttttactg
    14341 cccgcgatgt atttaatggg caacccggtg caaaatgtcg gtcgctgcct ggggacagcc
    14401 gaagtgaatg ccaaatatta cccgcatatc attacggtct gcgtgattaa cgcattgctg
    14461 tcgatctggg ttatgcagct tattgtttaa caaggagtta ccatgattga ttataccgca
    14521 gccggtttta ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc
    14581 gatgtcctcg tcgctaacgg caaaattatc gccgttgcca gcaatatccc ttctgacatt
    14641 gtaccggact gcacggttgt cgatctcagc gggcagatcc tctgtcctgg ttttattgat
    14701 caacacgtcc atttgattgg tggtggcggc gaagcaggtc ccacgacgcg cacgccagaa
    14761 gtggcgctaa gtcgcctgac ggaagcgggc gtcacgtcag tggttggtct gctgggcacc
    14821 gactctatct ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa
    14881 ggcatcagcg cctggatgct gaccggcgct tatcatgtcc cttcccgcac cattacaggt
    14941 tccgtggaaa aagacgtggc gattatcgat cgtgtgattg gcgtgaaatg cgccatctct
    15001 gatcaccgtt ctgccgcacc ggacgtttat cacctggcta atatggcggc ggaatcccgc
    15061 gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt tccacatggg cgacagcaaa
    15121 aaggcgttac agcctgtcta tgacctgctg gaaaactgcg atgtgccgat cagcaagttg
    15181 ctgccgaccc acgttaaccg caacgttccg ttgtttgagc aggcgctgga gttcgcgcgc
    15241 aaaggcggca ctatcgatat caccagcagc attgacgaac cggtcgcccc tgccgaaggt
    15301 attgcccgcg ccgttcaggc gggtattccg ctggcacgcg tcaccctcag ctccgacggc
    15361 aacggtagcc agccgttctt cgatgacgaa gggaatttaa cccatatcgg tgttgccggt
    15421 tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag actatgattt cagtatcagc
    15481 gatgccctgc gcccgctcac cagtagcgta gccggtttcc ttaacctgac cgggaaaggc
    15541 gaaattctgc caggcaatga tgctgacttg ctggtcatga cgccagaact gcgcattgag
    15601 caggtatacg ctcgcggcaa actgatggtc aaagacggca aagcctgcgt gaaaggaacg
    15661 tttgaaacgg cttaaacggt tgtcagccgc ccgaatggac gtagaatgcc cgcaagggcg
    15721 gctgacagag taaaacgtaa tggatgactg tggtgcgatt ttgcataata ttgaaaccaa
    15781 atggctttat gattttctga ccctggaaaa atgccgcaat ttttcccagg cggcagtcag
    15841 tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt gcgctggaac aggcgattgg
    15901 tgttgaattg tttaaccgcc aggtaacgcc gctgcaactc tcagaacaag ggaaaatctt
    15961 ccattcgcag atccgccatc tgttgcaaca gttagagagc aatctggcag agctgcgtgg
    16021 cggcagcgat tacgcgcaac gaaaaatcaa gatagccgct gcacactctc tttccctcgg
    16081 gctgttaccg tccattatca gccagatgcc gccgctcttt acctgggcaa ttgaagctat
    16141 tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa agtgactgta ttttttcctt
    16201 tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt cgcttatttg aatctcaatt
    16261 gttccctgtc tgcgccagtg acgaacacgg agaagcactt tttaacctcg cgcagccaca
    16321 ctttccgtta ctgaattaca gccgcaactc ctacatgggg cgattgatta atcgcaccct
    16381 gacgcgccac agtgagttaa gtttcagcac cttttttgtc tcttcgatga gcgagctttt
    16441 aaagcaggtt gccctcgacg gctgtgggat tgcctggctg ccggagtacg ccatccaaca
    16501 agaaattcgc agcgggaagc tcgttgtgct gaatcgggac gaactggtga tcccgatcca
    16561 ggcctacgca taccggatga atactcgcat gaatcccgtt gccgaacgct tctggcgtga
    16621 actgcgcgag ctggagattg tgcttagctg acattctcca gcatcgcact acaccaaccg
    16681 ccattatccc agaccagatc aaacgttagc ggttcaccgt cgtggtacag cattatctca
    16741 aggctgatcg ccagagtgtg gcagtacgct tctttaaaat taacctgcaa cgccgcaatc
    16801 cgtggctcat gaaataacag tgcagaacgc agctgacggc aataccagct ctggagatgt
    16861 ttgtcgctct ggttatgcac ttgccactcg ggcatgcctg aaacaagtaa tgacggtaat
    16921 gccgaatgag gagaattaaa aagagtgagg atatcgctgc ataccgattt acgcagcaat
    16981 gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa gtgattggcg catcattttg
    17041 cgcactcctg acgtttagca acatatgcgg cttgccctga acaccgggca ccactgtatt
    17101 gatgctcatt ttccatagcc attccctttt gttcattaca tccatgtcag tctgagacag
    17161 gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct atgaatatct actgactttt
    17221 caggaaaaaa gtcatgattt gataaaaatc atcaaactca aaaaaaaacc gctag
//
LOCUS       DAZFAH010000093        17021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFAH010000093 DAZFAH010000000
VERSION     DAZFAH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.093"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..286)
                     /locus_tag="ACHJWB_002841"
     CDS             complement(59..286)
                     /locus_tag="ACHJWB_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4314442.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            328..693
                     /locus_tag="ACHJWB_002842"
     CDS             328..693
                     /locus_tag="ACHJWB_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4314443.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            984..1547
                     /locus_tag="ACHJWB_002843"
     CDS             984..1547
                     /locus_tag="ACHJWB_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4314444.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARIKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1544..3205
                     /locus_tag="ACHJWB_002844"
     CDS             1544..3205
                     /locus_tag="ACHJWB_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4314445.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWVHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
     gene            3269..5206
                     /locus_tag="ACHJWB_002845"
     CDS             3269..5206
                     /locus_tag="ACHJWB_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4314446.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSFDRPFS
                     AASGNRRPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            5251..5472
                     /locus_tag="ACHJWB_002846"
     CDS             5251..5472
                     /locus_tag="ACHJWB_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314447.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            5418..8002
                     /locus_tag="ACHJWB_002847"
                     /pseudo
     CDS             5418..8002
                     /locus_tag="ACHJWB_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            7999..8325
                     /locus_tag="ACHJWB_002848"
     CDS             7999..8325
                     /locus_tag="ACHJWB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4314448.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            8335..8685
                     /locus_tag="ACHJWB_002849"
     CDS             8335..8685
                     /locus_tag="ACHJWB_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4314449.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            8682..9128
                     /locus_tag="ACHJWB_002850"
     CDS             8682..9128
                     /locus_tag="ACHJWB_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4314450.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            9125..9469
                     /locus_tag="ACHJWB_002851"
     CDS             9125..9469
                     /locus_tag="ACHJWB_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4314451.1"
                     /translation="MTEVDLYPYLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            9536..10252
                     /locus_tag="ACHJWB_002852"
     CDS             9536..10252
                     /locus_tag="ACHJWB_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4314452.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTMN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            10258..10632
                     /locus_tag="ACHJWB_002853"
     CDS             10258..10632
                     /locus_tag="ACHJWB_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4314453.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            10728..10937
                     /locus_tag="ACHJWB_002854"
     CDS             10728..10937
                     /locus_tag="ACHJWB_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4314454.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            10989..14231
                     /locus_tag="ACHJWB_002855"
     CDS             10989..14231
                     /locus_tag="ACHJWB_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4314455.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIENVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAES
                     DAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            14224..14565
                     /locus_tag="ACHJWB_002856"
     CDS             14224..14565
                     /locus_tag="ACHJWB_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4314456.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            14565..15263
                     /locus_tag="ACHJWB_002857"
     CDS             14565..15263
                     /locus_tag="ACHJWB_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4314457.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            15274..16017
                     /locus_tag="ACHJWB_002858"
     CDS             15274..16017
                     /locus_tag="ACHJWB_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4314458.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMSGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHASAFTGICNDLAAASACM"
     gene            16014..16595
                     /locus_tag="ACHJWB_002859"
     CDS             16014..16595
                     /locus_tag="ACHJWB_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4314459.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
     gene            16842..>17021
                     /locus_tag="ACHJWB_002860"
     CDS             16842..>17021
                     /locus_tag="ACHJWB_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314460.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPV"
BASE COUNT         3784 a         4097 c         5593 g         3547 t
ORIGIN      
        1 ggaattaata cagctaatgt ctgtgagttt ttataaattc agcaatataa agaaatagtt
       61 atatgaacag ccatcgcaga gcatactgtg tatcattctt ttttatagtc aactgacggg
      121 catattttat gtctgctgcc agctcccggc ggcaagattc aatgacccac gcagaaaaat
      181 tttctgaacc tttctggtca agagcgatgt taatttgttc aatcatctgg tttggaaatc
      241 ggatgttgcg ggttgttgtt ctgcgggtcc ggtttttcga tgacattttc tttcctctgg
      301 tgacaagtta tatggcgagg attttacatg gctgtgcttc gtacgttacc gggcagaatc
      361 aaaactctga acacccggcg ggtgaatgtc ctgaagggtg aacagcgtcg ggtcagtggt
      421 agtgcccggg tttccctcaa gcgccgtatc tggctcaggg atgccgggca gtgctgtctc
      481 tgtggtcgtg tggttgacct ctgtgatagt gaactcgatc accgaattgc acttcagttc
      541 ggtggtggta atgaggagac gaatctctgg acgctctgta ccgaatgcca tcgccagaag
      601 tcagtcagtg aaacggcgag tggtatgccg gacccgacgc tgccggaggt gtccggaggt
      661 catggcaggg cagacgatat catcggactg tgacccgccc cggggggggg atcatccggc
      721 gaaaaaaacg atcgccccgg acaccgcccc ccctctcacg cagagaaaaa attcccgttt
      781 cagggcagtt aacatgttaa ctggctgtcc gggcattttt gcggttttta tctttattat
      841 tcagtttgtt gcgtggaaaa aatgttaaca ggctttttca gcaaatgtta accaggcagc
      901 agttaacatt tgcggcatga gacgccggga aaaatgggct gaaccatacc cggctgagtg
      961 cgttatggac ccgggaggag gctgtgctga caacgcaaaa acgaaaattt gcgctggcgc
     1021 tcatgtccgg gaaaaacaaa acagcgtcag ccattgccgc cggttattcg gcgaagaccg
     1081 ccaggattaa aggctcgcag ctggcaaaag atccggaggt gctcgcgttt atagcccgta
     1141 aacagtgcga aacggtggag gtggatgagg ttcctgttta ccggcagaaa aaatcagagc
     1201 aggaggataa accccgtcgc cgtgaggcgg ctgcaatacc acagccggac gaaacaaatc
     1261 cggagatgcc accgcccgtg gtgatatctc ctggtattga gtatatggag gacggtcttc
     1321 ccgatccggt gaaagcgatg gggcgtcttc tggtggagaa cattaatacc gaccccaggc
     1381 tggcgctgga tgcggcttat aagctggcgc agttcacgca ccacaaaaaa ggggatgccg
     1441 gtaaaaaatc ggcaaaaggt gacgcggcga aaaaagcggc taaccgtttt gcggtgccac
     1501 caccaccccg actggtggtg aataatgata atgagggcaa cggatgatac ctgtgtggag
     1561 cacggcctgc ccggactggg cagagcgcct gaaaaagggg ctgtcgatta ttccggcccc
     1621 gatttatccg gatcaggctg cacatgcact ggcgattttt aaacaactgc ggattgtgga
     1681 tgcaccgggc agcccgacgt tcggggagtc ctgcgcacag tgggtgtttg acctggtggc
     1741 ggccctgttt ggctcctacg atgcgcagac cggtgttcgc catatcaagg aagtttttat
     1801 ccttatcccg aaaaaaaaca gcaagtccac gctggctgcc gggatcatga tgacggcgct
     1861 gttactgaac tggcggcagg cggcgggcta caccattctg gccccgactg tggaggtggc
     1921 ggctaacgcc tttaatcctg cgcgggatat ggtcagacgt gacgatgatc tggatgacct
     1981 ctgccaggtg cagacacata tccggaccat cacccacagg gtgacggaca ctacccttaa
     2041 ggtggtggca gccgatccga atacggtgtc cggtatcaag tccgtgggga cactgattga
     2101 tgaactgtgg ctgtttggca agcagtacaa agcggaagac atgttacgtg aagccatcgg
     2161 cgggcttgcc tcccgtccgg aaggatttgt ggtgtacaca accacccagt cgaatgagcc
     2221 gccagccggg gtgttcagac agaaactgca gtacgcccgg gatgtccgtg acggcaaaat
     2281 tcatgatccg cactttctgc cggtgatatt tgaacaccct cctgaaatgg tggaaagcgg
     2341 ggctcacctg ctgatggaaa acctcgccat ggtcaatccg aatctcggtt attcggtgga
     2401 tgaggctttt ctgtaccggg agtaccgtaa agcccgggag gctggtgagg aagcatttcg
     2461 tggcttcatg tcaaaacatg ccaatgtgga aattggtctt gccctgcgtt ctgaccgctg
     2521 gacgggtgcg gatttctggg agcagcaggg caggcgcgtc agcctggacg atatcctgca
     2581 gcgcgctgat gtggtgacgg tggggattga cggcgggggc ctggatgatc tgctgggaat
     2641 gtacgtgatt ggccgtgaca gggaaacccg cgaatggctg ggctggggcc atgcctgggt
     2701 gcatgaaacc gcggtggtca gacggaagag tgaggcatcc cggtttcagg attttgtggc
     2761 ctgtggagac atgacgattg tccgtcgggt cggggatgac acggcggaag tggcggagta
     2821 tgtgcgtcgt attcatgagg ctgagttact ggatcatatc ggtattgacc cgtcaggtgt
     2881 ggggcagatt ctggattcac tggcggaagc cgggatcccc gatgagagtg tggtggggat
     2941 aagccagggc tggaagctgg gcggggccat caaaaccacc gagcgcaaac tggctgaagg
     3001 ggtgctgatt cacggtgatc agcccctgat ggcctggtgt gtcggtaatg cccgggtgga
     3061 gcctaaaggt aacgccattc ttatcaccaa acaggccagc ggacggggaa aaattgaccc
     3121 gctgatggcg ctcttcaatg cggtgtccct gatgtccctg aatccggaac cgaaaaagaa
     3181 agcgtatgag gtttttttca tataaccctg ctcaccctgt aaccatcatg aaccgctgcg
     3241 gcggtttttt tattttcagg aggctgatgt gactcttaaa cgggcctgtt ccctgctgac
     3301 ggtgaaatcc ttcagtgagg atgaacgggt gatcaccggg attgcgtcaa cgccttctcc
     3361 ggatcgggat ggtgacatcc tggagccgga gggggcggag tttggcagta cgatcccgtt
     3421 tctctggcag catgaccatt cccgccctgt aggccagtgt acggtgcgtc gggtcaggga
     3481 agggctggaa atcacggcaa tgctggtgaa gccggagccg gggatgccct cccagatggc
     3541 agcccggctg gatgaggcct gggctgccat taagaccggg ctggtcaggg ggctttctgt
     3601 gggcttccgg ccccatgaat acacttatct ggacggaggc ggactgcatt ttctgcgctg
     3661 ggagctgatg gaggtgtctg ccgtcaccgt gcccgcgaat gcggaatgca ccatccggac
     3721 cattaaatct ttcgaccgcc cgttttctgc cgcgtccggc aaccggagac cggtggtgaa
     3781 aatcgcatct tctgccggcg cttcggcaca gtcaataacc tcttttcata aggaaaagtc
     3841 agcaatgaat actggtgaac agattaaaag ttttgaaaac aagcgtgcgg cgctggcagc
     3901 ctcccttgag gagatcatga acaaagccgc agaggagggc cgcacactgg atgtggaaga
     3961 ggaagagcac tacgacaaca ccgcagcgga aatccgtcag gtggatgcgc acctgaagcg
     4021 tctgcgtgaa ctggaagccg gtaaggccgc cacggcgcag ccggtgaaac aggccggtaa
     4081 cgggaatgtg gccacggtgg cttcagcgcc ggtgatccgt gttgagcaga aactggagaa
     4141 ggggattggt ttcgcccgct ttgccaaatc actggccgcg gctaaaggtg tccgctctga
     4201 agccctggaa gtggcccgtc gtcagtatcc ggatgacagc cgtctgcatc atgtcctgaa
     4261 atcggcggtg ggggcaggga ccaccacgga cccgcagtgg gcaggcagcc tgtctgaata
     4321 ccaggaatat gcgcaggact ttattgatta cctgcgtccg cagaccatta tcgggcgatt
     4381 tggtcagggc gggatccctg cacttcgtca ggtgccgttc aatatccgcg tgcatgccca
     4441 ggtgtccggt ggtgctgccg gctgggtggg ggagggtaag gccagacccc tgacgaagtt
     4501 tgattttgaa tccatcacct tcagtcatgc gaaagtgtcg gccattgcgg tactgacgga
     4561 agaattgatc cgtttttcca gtccggctgc tgatgcactg gtccgtaatg cgctggcgga
     4621 agcggtggtg gcgcgtctgg atacagactt tgtggacccg aaaaaagccg cagtggcaga
     4681 tgtctccccg gcgtccatca cccatgatgt gaagggcacg gcatcaaccg gtaacccgga
     4741 tgcggatgca gaggcggcgt ttggacagtt tgtggcagca aacctgcagc ccaccggtgc
     4801 ggtctggctg atgtccagca ccaatgccct ggcactgtcc atgcgtaaaa atgcgctggg
     4861 tcagaaggaa tacccggaca tgaccctgct gggtggctcc ttccaggggc tgccggtgat
     4921 tgtctcccag tacgtgggtg accagctggt gctggtgaat gccccggata tttatctggc
     4981 ggatgacggc ggcgtggcag tggatatgtc ccgcgaggca tcactggaaa tgcagtctga
     5041 gccgaccggc gacagtacca cgccgtcccc ggtggagctg gtttccatgt tccagacagg
     5101 cagcgtggcc atccgtgcgg agcgctggat caactggcgt cgtcgccgta ccgcggcggt
     5161 ggcggtgatc accggagtga actacggcag tgcgtccggc ggctgagtct gatgaggagg
     5221 acgggaggcg tgcgcctccc gtaacaggtt atggcaaaga tccgatatct gcagggcacg
     5281 catgatgccc gggccgggga tatccgtgat gtggcacagc cgtgtgcgga ggtgctggtt
     5341 cgcctgggga aggcggagta catcacagtg cgacgtccgg caggtcagaa aaagaaacgt
     5401 gatgcggagc atggcgaatg tggaaccttt tgcggcgaac ccgaaaaaac cagaaatcag
     5461 gacgtgatgt aaaagaggtg ggctggacca gcctgtttca ggcggtggct gagccttttg
     5521 ccggcgcctg gcagcagggc gtgaaagccg atccggaaag tgtcctctcc tttcatgcgg
     5581 tgttttcttg catttcgctg atatcccagg atatcgccaa aatgcgactg cgcctgatgc
     5641 agaccgatac acaggggatc cgccgtgaaa aacggcagga ggatattgcc cgtctctgtc
     5701 gtcgtcccaa tgcacagcag aatcgtatcc agttttttga actgtggctg aacgccaaac
     5761 tgcgtcacgg caatacggtg gtgctgaaaa tccgtaactc ccgggggcag atcaaagaac
     5821 tgcgtattct ggactggagc cgggttgaac ctctggtggc ggatgacggc gaggtgttct
     5881 accgcatcac gccggaccgg aactgcggga tcacggaggc ggtgacggtg cctgccaggg
     5941 aagtgatcca cgaccggttt aactgttttt ttcatccgct tgtggggctg ccgccggtgt
     6001 atgctgccgg gctggcggcc acgcaggggc atcatattca ggcaaattca acgtcttttt
     6061 tcagaaatgg cggcaggccg tccggggtga ttgagatccc cggcagtata acggaagaaa
     6121 atgcgaaaaa actgaagagc aactgggaca gcgggtatac cggcgaaaat gcggggaaaa
     6181 cggcgatcct gagcaacggg gcgaaatata accccacgac gttttcaccg gtggatgcgc
     6241 agacggtgga acaactgaag atgacggctg aaattgtctg ttcggtgttc cgtgtcccgg
     6301 cctacaagat tggcgttggc cagccgcctt ccagtgacaa cgtggaggcg ctggagcagc
     6361 agtattattc ccagtgtctg cagacgctga ttgagtccat tgagctgtta ctggatgagg
     6421 cgctggaaac aggggaaaac gagagcacgg agtttgacgt caccacgctg ctgagaatgg
     6481 acagcgaacg tcgcatgaaa acgctgggtg aatcggtgaa aaatacgctt ctcacgccca
     6541 atgaggcccg taaacgtgag aacctgccgc cccttgccgg cggtgatgca ctgtatcttc
     6601 agcagcagaa ctacagtctg gaggcgctgt cccgtcgtga tgcccgtgag gatccgttcg
     6661 cgtcttccgg gaaaacgtcg tcaggcacac gccaggaggg cgcgtctgac ggtaataagg
     6721 ccataagcga aacagagcgt gatgcggtga aggcgatgtt cagggggata ctgcgaaaat
     6781 gacagagcgt gaactgtcca ttatccgtgc gctgggtgaa gaattctcca cggtgctggc
     6841 ggatttacag cgcacatttg aggggaagat ggcctcgcag gcacaagcgt ttgaagagaa
     6901 actgacttcc ctgtcggcgg tattacagaa gcatgtgacg gtggatgagg tgcgtccggt
     6961 tctgcaggcg atggtggatg acgctgtggg ggccattccg gtaccgcgtg atggtcgtga
     7021 ttatgatccg gatgtactgc agcaggcggt gaatgatgcg gttgcgaata ttccggtacc
     7081 ggcggacggc aaaagtatta cccccgatga tgtgcgtccg atgcttgaac agatggtgaa
     7141 ggaggcggta agccatattc ctgttccgcg tgatggtcgt gattacgatc cggaagtact
     7201 gaagcaggcg gtgaatgatg cggttgcgaa tattccggta ccggcggacg gcaaaagtat
     7261 tacccccgat gatgtgcgtc cgatgcttga gcagatggtg aaagaggcgg taagccatat
     7321 tcctgttccg cgtgatggtc gtgattacga tccggaagta ctgcagaagg cggtgaatga
     7381 tgcggtcgcg aatattccgg taccggcgga cggcaaaagt attacccccg atgatgtgcg
     7441 tccgatgctt gaacagatgg tgaaagaggc ggtaagccat attcctgttc cgcgtgatgg
     7501 tcgtgactat gatcccgatg ttctgcagaa ggctgttctg gatgcggtga gagccctgcc
     7561 ggctccgcag gacgggcgtg atgccacggc actggaaata atccccgcca ttgacgatca
     7621 aaaatccttt ccccggggca cgtatgccac acaccagggc ggactctggc gggcgtatga
     7681 aaaaacgcac gggatgcggg gatgggaatg cctggttgac ggggtggccg atattgacgt
     7741 cagcatgacc ggcgaacggt cgttcactgt ggtggtccgg cagagcagtg gccagcgtac
     7801 ggaaaaaaca ttttccctgc cggtgatgct ctaccgcggt gtgttcagag ccggtgaaac
     7861 ctaccacccc ggcgatacgg tgacgtgggg gggctcgctg tggcactgca acagtatgac
     7921 cggtgataaa cccggagaag ctcattcatc aggctggacc ctggctgcaa aacgtgggcg
     7981 ggatgcagga ggcggaaaat gacggcatta ctgacgctgg aagagatcaa ggcacatctg
     8041 cgtgtcgacc atgacgcgga tgatgacatg ctgatggaca aggttcgtca ggctaccgcc
     8101 gtgctgctgg cctacattca gggcagccgg gataaggtga ttcgtgagga cggtgaactg
     8161 atcccgggcg aggcattaac ccggatgaag ggggctgcca tgcgactgac cgggatgctg
     8221 taccggaatc cggatcttgc ggagcgggaa gaactgcttc agggggagct gccgttttct
     8281 gtttccgtgc tgatttacga tttgcgttgt ccgacggtgt tatgaggagg gggaatggca
     8341 atatctgcag gtcgtctgac acagatgata agtgttctga acccggtgtt aacccgtaat
     8401 gctgccggag aaatgacgga agaatgggtg tcatgcggga aaattcatgc ggatatccgt
     8461 ggcaggagca gccgggagcg gatgcagtcc ggtgcggaaa tggcgcaggc ggaaatccgc
     8521 atctgggtgc gcggtcagtc cggtcgggaa atcacggcag cgtcacgact tcatgtgctg
     8581 agtggtccat ggcgtgaccg gatcctgaac gttgtcgggc tgcccgtgcc ggatgcgacc
     8641 ggcgggcgtc tggaaattct ctgtcggctg ggaggtgaaa aatgatcgaa accctgctgg
     8701 atttttcggg gctggaggac atcagccgcg atttgcagct tctgagtggt gcagaaaata
     8761 accgggtgct gcgtgaggca acccgtgcgg gtgcgaatgt gctgaaagaa gaagtggtgt
     8821 cacgggcacc ggtacgcagg ggaaaactgc gccgcaatgt ggtggtcctt tcccggcgct
     8881 cccgcgatgg cgggatggaa tccggtgtgc atatccgggg tgttaatccg gacaccggta
     8941 acagcgataa caccatgaag gcggataacc cgcgcaacgc tttctactgg cggtttgtgg
     9001 aaatggggac cgtgaatatg ccaccgcacc cgtttgtgcg cccggcgttt gatgtgcgca
     9061 gtgaacaggc agctcaggtg gcgattgcgc ggatgaaccg ggccattgat gaggtactga
     9121 gacgatgacg gaggtggatt tgtatcctta tctggcgcat cttgccggcg ggcaggtgta
     9181 cccgtatgtg gtccccctgc tggatggcag gccgtcggtg gcgcttccgt gggtggtttt
     9241 cagcctgatt tcatcggtgt cggcggacgt gatgggcggg caggcggagt cctcagtgtc
     9301 ggtgcagata gacgtttatg ccgggactgt gacgcaggcg cgtcagatac gtcaggacgc
     9361 ccgtgaagcc ataatgctgc tggccccggg atccgtcagt gaaatgcagg actatattcc
     9421 ggaaaaccgc tgttaccgtg caaccctgga gtttcaggtc acggtgtgac tttttctttt
     9481 tttctacaaa acccataccc cgccgcgtgc gggtttttta ttatcaggag gcagaatgtc
     9541 tgctttgtat gaacgctcac agctgacgca ggtgatgatt tcatctgccc cggcgactgc
     9601 tgaaaccatg gagaaggcgg aatatctgcg cctggactgc accatcaagg aagtccagtt
     9661 caccgccggt cagaaacagg atattgatgt gaccacgctc tgctccacag agcaggagaa
     9721 catcaacggt ctgggggcgt cgtccgagat ttccatgtcg ggtaattttt atctgaatca
     9781 ggcccagaac gccctgcgtg atgcctatga caatgacacg gtgtatgcgt ttaaggtgca
     9841 gtttccgtcc ggtaagggct ttaagttcct ggcggaagtg cgtcagcaca cctggtcatc
     9901 cggtaccaac ggcgtggtgg ctgcaacgtt ttcacttcgc ctgaagggta aaccggtgtc
     9961 ctatgtggta ccgctggcgt ttgtaaaaaa tctggagaag acacttacca tgaataccgg
    10021 tgcgctgctg acaatgtcag tcagtgtcaa cgggggaacg ccgccttata aacacgcctg
    10081 gaagaaggat ggtcagccgg tagagggaca gactactgac actttcagta aagccaatac
    10141 gcagtcaggt gataaggggg cttatacctg cgaggtaacg gattctgcag aacagccgca
    10201 gagcattacc tctgatgcgt gtacagtaac ggttaatggt gcgggcggat aaggcttatg
    10261 gcaaaagatc tgaaaacact ggcgctggcc agactgtcgg ggttccgtca taaaacggtg
    10321 aaggtgccag aatggagaaa tgtcagcgtg gtgctgcggg agccttcggc agaggcctgg
    10381 tatctgtggc aggaagtgct caatggtgat ggagaggatg acgataccct gtcggtggtg
    10441 gcgaaaaccc gccgtaacct ggaagcggat gtgacgctgt tctgcgatgt cctgtgtgat
    10501 actgacctgc aacgggtgtt cactccggac gaccgtgagc aggtgctggc cgtctatggt
    10561 ccggtacatg cccggttgct gcgtcaggca ctggaactga tcgctgatgc agagtcggcc
    10621 agaaaaaagt agcccgcccg gaaattcgct ttctgatgcg acttgcgctc cgtctggggc
    10681 gcaccttatc cgaactgcgc cacagcctga gtgcgagcga ggcgatgatg tggatggagt
    10741 tcgacagggt atccccgctg ggtgatgagc gcggggatat ccgtaatgca cagatcgtga
    10801 aagcggtttt cggggcacag gggatgaatg ttgcactgaa ggacgccatg ctctgctggg
    10861 gcgaggatga ggataagccg gaggtggatc cgtttgcggc gctggaagac gcgctgagcc
    10921 ttgcagcaat gtcataaata atgatgaaaa cctgctgtgg caggtttttt ttgcccggag
    10981 aaaggtgaat ggcgacgtta cgtgaactga ttatcaaaat ttcggcaaat tcacagtcat
    11041 tccagtcgga gatccagcgg gcgtcccgta tgggcagtga atattaccgg accctgcaga
    11101 atggcggacg tcaggctgct gcggcagcca gggagcagcg ccgggctctg gctgagctgc
    11161 acagccagtt gacggaaatc cgcgcttcgg ctgtcggaat gaccggtgcg tttgccggtg
    11221 cctttgccac cggacacctg atttcgctgg cggatgaatg gagctccgtg aatgcccgtc
    11281 tgaaacaggc gtcgcagtca tcggatgaat ttgcgtcatc acagaaagtg ctgatggata
    11341 tcagccagcg gacaggcacc gcattttcgg ataatgcggc cctgtttgcc cgttcggctg
    11401 cctcgatgcg tgaatatggt tacagtgctg atgatgtgct gaaggtgacg gaggccattt
    11461 cgacagggct gaaaatctcc ggtgccagta cggcagaggc gggctcggtg atcacccagt
    11521 tcagtcaggc gctggcgcag ggtgtattgc gtggtgagga gtttaattcg gtcaatgaaa
    11581 gtggtgaccg gatcgtacgt gcactggctg cgggtatggg cgtggcccgt aaagatctga
    11641 aggcaatggc ggatgacgga aaactgacag cggataaagt ggtccccgcg ttaatcagcc
    11701 agctggggat attacgtgat gaatatgcgg ccatgccgga aacggtttcc agtagtatca
    11761 caaaggtgga aaacgccttt atggcctggg tgggcggtgc gaatgaggcc agcggggtga
    11821 caaaaacgct ctccggcatg ctgaacggtg ttgccggaca gattgagaat gtggcaacag
    11881 ccgtgggcgc gctggttgcc gtcggggttg cccggtactt tggcaatatg gcctccggag
    11941 cgatgtctgc cacggcagga cttgtgacgg ctgcacgtaa tgaagttgca ctggcggaag
    12001 cacagttcag gggaacgcag attgccacgg cgcgggcaag ggcagccgtg taccgtgctc
    12061 agcaggccgt ggcggcagcc cgcgggacgg agatgcagat tgctgcagag gcccgtctgg
    12121 cggccacaca ggaacgcctg aacagaaata ttgctgccag aagcgccgcc cagaatgcgc
    12181 tgaacagtac aacggcggtg ggctcacgtc tgatgagcgg tgcgctgggg ctggttggtg
    12241 gcgtacccgg actggtgatg ctgggggctg cagcatggta cacgctgtac cagaatcagg
    12301 agcaggccag ggagtctgcg cgccagtatg cactgacgat agatgaaatc gcgcataaaa
    12361 cgccgtcaat gtctttgcct gaagcctcag ataatgaagg acgaacacgg gcggcgctga
    12421 cagagcagaa ccggctgatt gatgaacagg ccagtcgggt gaaatccctg caggaaaaaa
    12481 tcgcaggata tcagtatgtt ctggcgaacc cgggctggac gaccggtgac ggattcatga
    12541 taaaccatct gacctcggtg aagaccgtaa cggaagggct tgctcaggca acagagcagc
    12601 ttgccgttga gcagtcccgt ctggcacaga tgcaggaaaa agcgcagtcc attcaggatg
    12661 tgcttgccgg gctggaagac cgtcgtgtgg cgttaattcg tcagcaggcg gcagagcaga
    12721 ataaggtgta ccagtccatg ctggttatga atggtcagca tacggaattc aaccgtctgc
    12781 tggggctggg taatgaactg cttcagcagc ggcagggact ggtgaatgtg ccgttacggc
    12841 tgccacaggc cactctggat gataaacagc agagtgccct gacaaaaaca gagcgtgagc
    12901 tggccctgtc cagactgaaa ggggaagaaa aagagcgtgt ccggctgggg tatgcggcgg
    12961 atgacctcgg ttttgtgggt gatccgtatc aggaggcgag acagcgttat atcagtaatg
    13021 ccctggaagc ctggcgcaat aacgaggcga ataaacccaa atcccggggt ggaaaatcag
    13081 agacggaaaa agcggaagac agtttttccc ggctgctgaa gcagcagaaa gagcaactgg
    13141 cactggtggg gcagaataca gagctggcga agctgaaata ccagactgcg cagggcgaac
    13201 tgaaaaccct gacggagatg cagaagcagg agctgctgcg taatgcggcc ctgattgacc
    13261 agcaaaaaat ccgggaacag ttgcgatccc gggaagagac actgaagaat gagaatgcgg
    13321 ctgcgcgtgc gtcgaatgat gctgaactgc tggggtacgg gcagggggaa cgagccagag
    13381 aacgcatgcg ggagttgcag cagatccgcg acagcttccg ccagaaggat gcggaccttc
    13441 agtctcagta tcagaccggg gatatcagtg aggattttta cagacaggct ctggcgcaga
    13501 atgcacagta tctgagtgaa cgtctgaaag agcaggaagc cttttatgcc gaatcggatg
    13561 cgcagcgtgc ggactggcag aaagggctgc aggagggatt cagtaactgg gtggataatg
    13621 cgtccgatta cgcctcacag gcagcacagc ttgcgacgga gggtatctca gggatggtga
    13681 ataacatcac ggagatgctg aacggaaata aagtggaatg gcgcagctgg gcctcatcag
    13741 tgctgcagga aatatcaaaa gttcttatga atgccgcgat tgtcaacgga attaagacgg
    13801 cggcaaacgg tatgtccggt gcgggaggat ttctcggcag cattggtgac tggctgggcg
    13861 gagcggtggc caatgcaaaa ggcggcgtgt atacctcggc aaacctgagt gcgtacagca
    13921 acagtattgt ggatacgccc acgtactttg cctttgcaaa aggggccggg ctgatggggg
    13981 aggccggacc tgaagccatt atgcctctga cgcgggcggc ggatggctcg ctgggtgtgc
    14041 gcgcggtggg tagtatgaac ggcagtgcgg gtctggtgta ttccccggtc taccatatcg
    14101 ccattcagaa tgacgggact aatggccaga tagggccgga ggcggcaggc agtcttgtgc
    14161 aactgattga ccagcgggtg caggcggtga tgctgtccat gcgacgtgac ggaggaatgc
    14221 tgagtggctg agataaaaac gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag
    14281 aagccgtcgg tggtgagggt gcggtttggt gacggttatg aacagcgccg ccccacaggg
    14341 ctgaatcctc aactgaagac gtttcaggcg gtgttccggg tgacggatga gtcaaccagg
    14401 cgctggctgg aagagttttt atcgtggcat ggtggttacc gtgccttttt gtggcgaccg
    14461 ccgaaacata accggacggt gagggttgtg tgccgtgagt ggagtgtcac ggataatgcc
    14521 cggtacagtg atttcagttg cacgatagag caggtggtga actgatgcag gatattcacg
    14581 aagaaagcct gaacgagtcg gttaagtcag agcagtcacc gcgggtggtg ctctgggaaa
    14641 tcgacctgac ggtgcagggc ggtgagcggt attttttctg caatgagctg aatgaaaaaa
    14701 gggagccggt gacctggcag gggcggcaat atcaggcgta cccgattgag ggcagtggct
    14761 ttgagatgaa cggaaagggc agcagtgccc gcccgtcgct gacggtgtcc aatctgttcg
    14821 gtcttgtcac cgggatggcg gaggatttgc agagcctggt gggtgccacg gtggtccgcc
    14881 gccgggtgta tgcgcgtttt ctggatgcgg tgaactttgt ggcaggcaat ccggaggccg
    14941 acccggagca ggagctgact gaccggtggg tggtggagca gatgtcatcg ctgacggcca
    15001 tgacggcctc gtttgtgctg gccacaccga ccgagacgga cggggcgctg tttcccggtc
    15061 gcatcatgct ggcgaacacc tgtatgtgga cctaccgctc tgatgagtgt ggttacacgg
    15121 gcggggctgt ggcggatgag ttcgataaac ccaccaccga tatccgtaag gacagatgca
    15181 gcaagtgcat gcgcgggtgt gagatgcgcg gcatggtggc taattttggc ggtttccttt
    15241 ccattaacaa actttcgcag taaatcccgg tttatgacac agactgaatc agcgattctg
    15301 gcgcatgccc ggcggtgtgc gcctgcggag tcgtgcggct tcgtgataag cacgccggag
    15361 ggggagcggt atctgccgtg tgtgaatatt tccgcagagc cggaggcgta ttttcgtatc
    15421 gcaccggaag actggctgcg ggcagagatg tcgggggaga ttgtggcgct ggttcacagt
    15481 catcccggtg gtctgccctg gctgagcgag gctgaccggc ggctgcagat aaaaagcgca
    15541 ctgccctggt ggctggtcag ccggggtgac attcacaaat tccgctgtgt gccacatctg
    15601 acaggacggc gctttgagca cggggtgacg gactgttaca cgctgttccg ggatgcctac
    15661 catctggcgg gaattgatat gccggatttt gagcgtgagg atgactggtg gcgcaacggt
    15721 cagaaccttt acctggacaa tatggcggtc accggctttt accgggtgcc cctgtcctct
    15781 gcacaggcgg gcgatatcct gctgtgctgc tttggcgcat cggtggccaa tcatgccgcc
    15841 atttactgcg gcaacggtga actgcttcac catctgcctg aacaactgag taaacgggag
    15901 aggtattccg aaaaatggca acgacgaacg cattcagcct ggcgtcaccg ccactggcac
    15961 gcatctgcct tcacggggat ttgcaacgat ttggccgccg cctcagcctg tatgtgaaca
    16021 cggcagcgga agccattcgc gccctgtcga tgcagatgcc gggctttcgc cgtcagatga
    16081 acgaaggctg gtaccagata cgtattgccg gtgatgacac ggcaccggag gcggtgtacg
    16141 cccgtcttca cgaacctctg ggtgaggggg cggtcatcca tattgtgccg cgactggccg
    16201 gagccggaaa gggcggactg cagattgtgc tgggggcggc agccatcgtg ggctctttct
    16261 tcacggccgg agcatcgatg gcagcctggg gtgcagcgct gagtgccggc ggtttttctg
    16321 ccaccacgat gctgttttca ctgggtgcca gcatgatact gggtggtgtg gcacagatgc
    16381 tggccccgaa gccaaaaaca ccggaataca gggcaacgga taacggtaaa cagaacacgt
    16441 acttttcgtc gctggataac atgattgccc aggggaaccc gatgccggtg ccttacggtg
    16501 aaatgctggt tggttcacgg cgaatctccc aggacatcag tacccgtgat gaaggcggtg
    16561 acgggaaggt ggtggttatc gggcggcagg ggtaaagcat aaaaaaatcc cgcagtgtat
    16621 ggaggctgcg ggaacagaaa atgaagatta accacaggga gttttgtttt tattggcccg
    16681 aaaaaactgt aacgcccggg aatgatatct gccacggggg cgtacagaaa atgtgaagaa
    16741 attcagaaat tttattccgt catgacacag gcaccctccg gggtgcctgt cgtttttggg
    16801 gcataaacag attcagacat cagacaggag agggggacag agtgggtaaa ggtggcggca
    16861 aggcgcacac gccggttgag gcaaaggaca atcttaagtc cacgcagatg atgagcgtga
    16921 ttgatgccat cggtgaggga ccggtggaag gtccggtgaa ggggctgcag agtattctgg
    16981 tgaacaaaac cccgctgacg gacacggacg gtaatcctgt g
//
LOCUS       DAZFAH010000094        17002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFAH010000094 DAZFAH010000000
VERSION     DAZFAH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.094"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..1178
                     /gene="arnB"
                     /locus_tag="ACHJWB_002861"
     CDS             39..1178
                     /gene="arnB"
                     /locus_tag="ACHJWB_002861"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4314461.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1182..2150
                     /gene="arnC"
                     /locus_tag="ACHJWB_002862"
     CDS             1182..2150
                     /gene="arnC"
                     /locus_tag="ACHJWB_002862"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4314462.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2150..4132
                     /gene="arnA"
                     /locus_tag="ACHJWB_002863"
     CDS             2150..4132
                     /gene="arnA"
                     /locus_tag="ACHJWB_002863"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4314463.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4129..5019
                     /gene="arnD"
                     /locus_tag="ACHJWB_002864"
     CDS             4129..5019
                     /gene="arnD"
                     /locus_tag="ACHJWB_002864"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4314464.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQANSGNWDRQTLVDDIARGLHTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5019..6671
                     /gene="arnT"
                     /locus_tag="ACHJWB_002865"
     CDS             5019..6671
                     /gene="arnT"
                     /locus_tag="ACHJWB_002865"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4314465.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6668..7003
                     /gene="arnE"
                     /locus_tag="ACHJWB_002866"
     CDS             6668..7003
                     /gene="arnE"
                     /locus_tag="ACHJWB_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4314466.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7003..7389
                     /gene="arnF"
                     /locus_tag="ACHJWB_002867"
     CDS             7003..7389
                     /gene="arnF"
                     /locus_tag="ACHJWB_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4314467.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7383..7649)
                     /gene="pmrD"
                     /locus_tag="ACHJWB_002868"
     CDS             complement(7383..7649)
                     /gene="pmrD"
                     /locus_tag="ACHJWB_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4314468.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7759..9114)
                     /gene="menE"
                     /locus_tag="ACHJWB_002869"
     CDS             complement(7759..9114)
                     /gene="menE"
                     /locus_tag="ACHJWB_002869"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4314469.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGAEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9111..10073)
                     /gene="menC"
                     /locus_tag="ACHJWB_002870"
     CDS             complement(9111..10073)
                     /gene="menC"
                     /locus_tag="ACHJWB_002870"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4314470.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10073..10930)
                     /gene="menB"
                     /locus_tag="ACHJWB_002871"
     CDS             complement(10073..10930)
                     /gene="menB"
                     /locus_tag="ACHJWB_002871"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4314471.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10945..11703)
                     /gene="menH"
                     /locus_tag="ACHJWB_002872"
     CDS             complement(10945..11703)
                     /gene="menH"
                     /locus_tag="ACHJWB_002872"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4314472.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLISYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11700..13370)
                     /gene="menD"
                     /locus_tag="ACHJWB_002873"
     CDS             complement(11700..13370)
                     /gene="menD"
                     /locus_tag="ACHJWB_002873"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4314473.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLDDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13459..14754)
                     /gene="menF"
                     /locus_tag="ACHJWB_002874"
     CDS             complement(13459..14754)
                     /gene="menF"
                     /locus_tag="ACHJWB_002874"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4314474.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14833..15138)
                     /gene="elaB"
                     /locus_tag="ACHJWB_002875"
     CDS             complement(14833..15138)
                     /gene="elaB"
                     /locus_tag="ACHJWB_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4314475.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15193..15654)
                     /gene="elaA"
                     /locus_tag="ACHJWB_002876"
     CDS             complement(15193..15654)
                     /gene="elaA"
                     /locus_tag="ACHJWB_002876"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4314476.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15719..16636
                     /gene="rbn"
                     /locus_tag="ACHJWB_002877"
     CDS             15719..16636
                     /gene="rbn"
                     /locus_tag="ACHJWB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4314477.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16824..>17002
                     /locus_tag="ACHJWB_002878"
     CDS             16824..>17002
                     /locus_tag="ACHJWB_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO4314478.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDE"
BASE COUNT         3938 a         4606 c         4342 g         4116 t
ORIGIN      
        1 tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt ttgccttttt
       61 cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc gaatccggtt
      121 ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa
      181 atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg ctaatggcgt
      241 tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt tcaaccctca
      301 atatgatttc cttgttgggt gcaacgccgg taatggtgga tgtcgaccgc gatacgctga
      361 tggtcacgcc tgaagcgatc gagtcagcca taacgccacg cactaaagcc atcattccgg
      421 tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc gaacgttacg
      481 gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa gggcgacata
      541 ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt acctgtgcgg
      601 aaggtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg atgctgaaat
      661 ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca ccgcaggctg
      721 aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg attgccctga
      781 cacagttagc aaaattagag cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc
      841 agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg ccgcacgttc
      901 acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg
      961 cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc cgcgccgctc
     1021 acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat accgaatgga
     1081 atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc gatgcggacc
     1141 gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa atccaccctg
     1201 ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta ccggaattaa
     1261 tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg
     1321 atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa gcggagaaca
     1381 gccatattgt gtctattttg cttaaccgca attacgggca acattcagcg attatggcgg
     1441 gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc cagaatccgc
     1501 cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg gttgggactg
     1561 tacgccagaa ccgccaggac agctggtttc gtaaaaccgc ttcgaagatg attaaccggc
     1621 ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc
     1681 gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt atcccgattc
     1741 tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc gagcgtgagt
     1801 ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac ctggtgacct
     1861 gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt gcgattggag
     1921 gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca caatgggcgg
     1981 cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct cagtttatcg
     2041 gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc gcccgccccc
     2101 gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa tgaaaaccgt
     2161 cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg ctgccggtta
     2221 cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct tttatggttc
     2281 ggtggctcat ctggcggcgg aaagaggcat tccggtttat gcgccggata acgttaatca
     2341 tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct ctttttatta
     2401 tcgccatctt atttgcgacg agattttgca gctcgctccc gcaggtgcat ttaatctgca
     2461 cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc tggtgaacgg
     2521 tgaaacagaa actggcgtta cattgcaccg aatggtaaaa cgtgccgatg ccggggccat
     2581 tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc tgcatcataa
     2641 attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta aacacggtaa
     2701 tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca gaacgccgga
     2761 tgacagtttc ctcgaatggc ataagccggc atccgtactg cacaacatgg tacgtgccgt
     2821 tgccgatccg tggccgggtg ctttcagcta tgttggcaat cagaaattca ccgtctggtc
     2881 gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc
     2941 actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg cgggcgacgg
     3001 cattactatg cagggctcgc aattagcgca gacgctgggc ctggtgcaag gttcacgctt
     3061 gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc tcggggtaaa
     3121 tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt atgaagttta
     3181 cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccgc attttcactt
     3241 tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca aaaaatgtga
     3301 tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca acccgctgcg
     3361 cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg tgaagtaccg
     3421 taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg ataaatactt
     3481 cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct ggatttattc
     3541 ggtgtcaaaa caattacttg atcgggttat ctgggcctat ggcgaaaaag agggtttaca
     3601 gttcaccctc ttccgcccgt ttaactggat gggaccacga ctggataacc ttaatgcagc
     3661 gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag aaggttcacc
     3721 gattaagctg attgatggcg gaaaacaaaa acgctgcttt actgatattc gcgatggtat
     3781 cgaggcgtta taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg aaattatcaa
     3841 cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc tgctggcgag
     3901 cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc gcgttgtcga
     3961 aagtagcagc tactacggca aaggatatca ggacgtagag catcgtaaac cgagcatccg
     4021 caatgcccgc cgctgcctgg actgggaacc gaaaattgat atgcaggaaa ccatcgacga
     4081 aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat gaccaaagtc
     4141 ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc gcgtctgctg
     4201 gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg cccggacaat
     4261 atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat gctgcgctca
     4321 aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg gccaggtaaa
     4381 gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggc
     4441 ctgcacgcct gggatcacca tgcctggcaa gccaatagcg gtaactggga tcggcaaaca
     4501 ttggtcgacg atattgcacg cggccttcac accctggaag agattatcgg tcaaccggta
     4561 acctgttctg ccgctgcggg ctggcgtgcc gaccagcagg tgatcgaagc aaaagaagcg
     4621 ttccatttgc gctacaacag cgattgtcgt ggggccatgc cgttccgtcc actgctcgaa
     4681 tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga tgaagtgatt
     4741 ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat cctgcgagat
     4801 aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta tcagcataat
     4861 tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc tttaagcgaa
     4921 ctgttatcag ggacgttgcc gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa
     4981 ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta cgttacctta
     5041 tcggcctctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg cgtctgctct
     5101 ggcaaccaga tgaaacgcgt tatgcggaaa tcagtcggga aatgctggca tccggcgact
     5161 ggattgtgcc ccatctgtta gggctacgtt attttgaaaa acccattgcc ggatactgga
     5221 ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta
     5281 tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg
     5341 ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt gtctatgcca
     5401 tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg ggaatgtgca
     5461 gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga tttttactgc
     5521 tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg
     5581 tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc tttatttacg
     5641 gctggctggc ggttatcagt tgcgtactga cggttctccc ttggggactg gcgatagcgc
     5701 agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa cgctttgcac
     5761 tggatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc attgccggta
     5821 gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa aaccgcaagc
     5881 attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt ttctccgtcg
     5941 ctaaaggtaa attgcccacc tatattcttt cctgctttgc atctctggca atgctgatgg
     6001 cgcattacgc tttgctggca gcaaaaaata atcctctggc gctgcggatt aatggctgga
     6061 ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtggtctcc ccgtggggac
     6121 caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt gcctggtcga
     6181 ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa aagacctggt
     6241 cttttgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca attcctgaca
     6301 gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc
     6361 caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca tggagcctgc
     6421 aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc cttaattatc
     6481 cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt aatcaacatc
     6541 gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc
     6601 tcgccattcc gcccgcagat gccatcgatc gtcaggagcg tctggtgctg attcagtatc
     6661 gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg ccgggcagtt
     6721 gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac atatcgtgct
     6781 gtggctggga ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc tgctggtctt
     6841 gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg tctgggtgac
     6901 gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc
     6961 gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct gatatgggga
     7021 ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc ggcaagtcat
     7081 ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt tggcctggat
     7141 gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg ttggtataaa
     7201 acgcttcata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag ttatgtactg
     7261 gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct gaaagcacta
     7321 ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccat gacaaaacaa
     7381 cgttactgag ttttccctgc cactttacac tgccgttccc actcatcggg gctataactg
     7441 cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata aagcgcattc
     7501 tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac ttcggcgatc
     7561 atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc ttgtttattg
     7621 cagcacgatt ttttgaccag ccattccatt gcattatcct gtttgctaag agttttcaca
     7681 tcaatttaca gattaacaga taaactcttt atcaacggca atggaatggc agaaatgaga
     7741 agtgaaacgg agggttaatt attgctgacg ttgcacccac tcttttagcg cctgacgtga
     7801 aattttaata ccgccgtttt tcagttcagg cggcaacgtt aaccaacgca ccggttgctg
     7861 aaaacgggcc agcttatctt tcacccattc actaagatca acgctctcgt ggtcatactc
     7921 catcaccgcc accggtcgat gaccaaactc cttgtcggca acggggacga taaacacctg
     7981 caaaaccgca ggatgtgcag caattacgcg ctcgacttct tccggctgaa taccctctgc
     8041 gccactgaag aatagattgt ctaaacgtcc gacaatggtc agcttgccat tatgcatctc
     8101 accgcgatcg cgcgtagcgt accagccttc gtcattaacc agtgaaacca gttgcccgtt
     8161 acgccagtaa ccttctgcca tactggcagc ccgcagccac acttcattat taacgatttt
     8221 cacttcccga cccggcagcg gcgaaccaac gtctgccagg ccgtcggctt ctttcgcaca
     8281 caccgtggag gcaaactcgg tcagaccata gccgcaaaag caacgaatcc cctgctcgcg
     8341 cgcctgttcc gtcaactcga ccgggatagc cgcgccgcca agtaacaccg ctttcaggga
     8401 aacggaactg cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg aagcatgagt
     8461 acagcctgcc agcatttgct ccaatggctg tttatcacgc accgtcatcc gcgcaccagc
     8521 gtataaccag cgccacataa ttccctgacc ggagacgtga aacagcggta aagagagcaa
     8581 ccagtcatcg tgatcgccaa acggaatcaa cgataacacc ccttcggcac tggcaagatg
     8641 ggcctgataa gtatggacag cggctttcgg caacccggta gaacccgagg tcaacgtcat
     8701 tgagcacaga cgcgtcggct gccacgtagc ggcatgtgcg ccttcaaccc gctgaatgtg
     8761 cagcgacgtt aacgccggaa acgtgttttc cccatccggc accagcgcaa attgcagcgt
     8821 cagattgggc agcaattctt caagcaacgg ttgcggcagc tgagggttca cgggcaacac
     8881 ccgcgccccg cattgcagta acgccagcca ggcgagcagc gtttgcggcg tattccacgc
     8941 ccgcaacatc acgccgctgc cctcaacaac gccctgcgcc gcaaagcccg tagctaactc
     9001 atcgacgcga gcacaaagct cgcgccagtt aagttgctcg tcattaagac gtaaggcgat
     9061 ggcttctccc cgcacttgcc gccagtgacg ccacggccag tcagagaaga tcataacaac
     9121 cgctccagtg catcaacttc cacgacaggc agcgtgctac ccggccagcg acgtacctgc
     9181 tgcgcctgca tcagatccag cgtgtccagc cctggaatgg tgtccggcgt taaccaggcg
     9241 gcaatccgcg ccagttgcgt taagcctaag ctcgattcaa tggaagaact gatcaccgcc
     9301 gtcagcccca gcgcgtgcgc cgcctgtacc tgctcgcgta ctttctccag actgcccgtg
     9361 agcgtgggtt tgataactac cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc
     9421 tcgcgcaagc tttcatccca ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa
     9481 tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata atccgggtta
     9541 acatatttgg caaactgctg gcctttcagc ggtgtccagg cgcgatttgc gtcaagacgc
     9601 aaatgcagat ccggaattgc ctccagcaac agattaacca ccatgccgtc gcgcacggct
     9661 tcgtacaatc ccactttgac cttcgccact ttctcgcctg gcatatcagc aagtttgagg
     9721 atcagatcgt caggatcgcc attacacagt ggtgccgcac ggtaattggc cgcctgcggg
     9781 agcgtatctg ccagctccgc caatgcacag cttacgccaa aggcaacgga agtcatctgc
     9841 ggtagctcgc aatcgcctgc cagccagtta tttacccagg caagcagcac gctttgcgcc
     9901 tcttcccagg tttcctggct gaagcccggc agtggggaga tctcccccca cccttcgcgc
     9961 tcgccctcac gcaggcaaac gtacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga
    10021 accacccccg cgtccatggg gatctgccag cggtatacct gcgcgctacg cattacggat
    10081 tccgtttaaa tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg cgaccttcct
    10141 gaccttcttc cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc tgcagccccg
    10201 cctgcccgtc acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt
    10261 tttgcagcat ttcgcggcac caacggacgg tttctttttc cagatccgcc agcggtacca
    10321 cggtgttgac aaggcccata tccagcgcct gttttgcgtc gtactgacgg cacaggaacc
    10381 agatttctcg cgcttttttc tgcccgacga tacgagccat gtaggaagcg ccccagccgc
    10441 cgtcgaagga accgactttc gggccagtct gaccgaagat ggcattatct gctgcgatag
    10501 tcaggtcgca catcatgtgc agaacgtgac caccgccgat ggagtagcca gccaccatcg
    10561 cgacaaccgg tttcggacag gtacggatct gacgctggaa gtccagcacg ttcaagtgat
    10621 gtacgccgga atcatcttta tagccgccgt aatcaccacg cactttctgg tcaccaccgg
    10681 agcagaacgc tttatcgcct gcgccagtca gaatgatcac gccgatattg tcgtcataac
    10741 gcgcatctgc cagtgcctgg atcatctctt ttaccgtcag aggacggaag gcattgcgca
    10801 cctgcggacg attaatggtg atttttgcga taccgtcggt ggatttttca taacgaatgt
    10861 cctcgaaacc ttcggagcag tcgtgccatt caaccggtgc gtaaagcatt gcttcatcag
    10921 gataaatcat attgtgtcct ttagtcagaa acgcaagatc tgcgccagac ttgcgattac
    10981 gccagcggga ttttcccgat gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc
    11041 agccagttcc gccgccaggg cgcggaattt gctgtcacgt tcaccacata aataataaaa
    11101 cgcaaatgtg cgggcgctaa ggttagcacg taaatcaggc tggacggcga gagaagtcgc
    11161 ctccagcatg gcggcaaggg ttgcgccatt attgttgctg cgcagcacca ccagctcccg
    11221 gcgttgatcg tcattgagcg aggcaaaaac aggctgttga taccagtcgg caaataccgc
    11281 cgttaacggt tctgtgcaaa aacgctgcgc ccattggcga tcggaacgct gacgttccgc
    11341 acgttgttca gcattttgca gccccggatg cccgccttcg acaataaccc cacaaagccc
    11401 cgtcagcccc tggcaagccg ccatcatcgc cacccgtccg ccaagcgagt accccaccag
    11461 ccagaagtca aggatgttgt aactaatcaa ggttttacgc agtaagtcgg tgacatcatc
    11521 aaatccatcg acgctaatcg ccgccgaacc accatgacct gggagatcaa catacaaccg
    11581 tgagtagtcg gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc
    11641 gtgcaaaaac accagccagg gtaaacctgg ttttccgtgt tttgcctgcg cgtgcaggat
    11701 cataaatggc ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta
    11761 accaccattt caatcaccgt ggtggttggc gtgcgccagg cgtcggcaaa tgccgtttca
    11821 agttcctgcc agttttgcgg acgatgatat ttcagctcga acatcgcggc ggcgtgctca
    11881 aaatggacgt tttgcggcat cagatagaaa cgctcacgct cgctttgcgg cgttggcaac
    11941 agcgagaaaa tttgcccgcc gttgttgttc accacaatta ataccagcgg cgcagaaacc
    12001 tgacgcaata acgccagcgc gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc
    12061 agcgtcggtt tgccgcttgc ccgctgaacg cctgcggcgg tcgaaagcag tccgtcgata
    12121 ccgctggccc ctcggttgct gtacaccggg taacctgccg gaagttgcga aagcgcatca
    12181 atcagacgta ccaccaggct gttaccaaca aacaattgcc cctgttcagg cagatagtcg
    12241 cagatgcgat gcgccagttg cgcttcgcca aacgcatcac ggcgggcaat aaccgcctgc
    12301 attgcctgtt ccgccaggcg cgggatttca acgcaccagg gctggcgttt ttctgccgga
    12361 tgcagctcca gccagtcggc aatattggca attaagcgac gtccgcgatg gtgtgccgga
    12421 tcaagtcgcc cttcaatgtc atcaacaatc cagtactctt ctggttcaca gcttgcctgc
    12481 cattgcagga gccgtttgcc cgtcaggctg cttcccagtt gcaccacaat ttgcgcctgc
    12541 tgcagctcgc tggtcgcttt ggcattgcct aaccaaagat cggcacacgg cagcggctgc
    12601 ccggtttgtg acagcacatc gccaatcagc ggccagccaa gagtttgcgc ccacagggca
    12661 acttttttgc cctcttccgc actcatgcgc ccggcaacca ccacgccgcg cttttgtcgc
    12721 cagaagaacc agtcgcgctg tttttcactt tccagacgag gcgcttcacg cagccacggt
    12781 ttgtcgtcct gccaccagtc atccagacgc tgttgccagc taagcccggt atcgtccatt
    12841 tcgccataca gcggttcagc aaacgggcag ttgatatgga ctccccccgc atgaagcgta
    12901 ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg ggatatcccg ggtcgggcgc
    12961 ggcaatgaaa tactgtgcgt ggggtgagag gcgaacattc ccggctggcg aattgcctga
    13021 ttcgcgccgc agtcaattag ctccggcggg cgatcggcgg ttaacaggat cagtttttct
    13081 ccggttaacc cagcttcaat cagtgccgga tagagatttg ctaccgccgt gccggaggtc
    13141 acaatcaccg ccaccggctg cttgctgact ttcgccagcc ccagcgccag atgtcccagt
    13201 ccacgctcat cgaaatgggt gtggtgaatg aatgtggaat tctccgccgc cgctaacgtt
    13261 aacggtgtag aacgcgagcc aggggcgata cagatgtgtc tgacgccgtg acgcgttaat
    13321 gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc
    13381 ggtatcaata ttgcggctaa gtataaggag ctacaaaaat agattattga tatgaatcgg
    13441 taatgatgcg actcattact attccatctg taataaagta cgcagccctg ccgctttgtt
    13501 gtcgatttcc tgccactctt gctcggggtc ggaaccacgg acaatgcccg cgccagcata
    13561 taatcgcacg acattgccgc taatttttgc tgagcgcagg gaaacgcaga attcgctttg
    13621 ttgtaatgag agatagcccg cagaaccggc gtaccattct cgggtgaacg gttcgtgacg
    13681 ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca actgctgccg ttggctgcaa
    13741 ctgatgtaaa cagatcacat catccgcttt gttgagtgaa gtccagatac agcggcgaag
    13801 atgctgcact ttacgcagac gcagtacctg cggcggtaaa acatccagcg tctgggtatc
    13861 ggcctgtaat cgttggcaga tatcttccac caccagcatg ttctcgcgct ggtttttatc
    13921 atccgccatc agccactctc ctaactgctg cgcctgctta tcatcaggat tatttgctac
    13981 tgttcccgcc agcgcttcag tacgcagcgc tttgtcacgc cgccgccata accgttccgg
    14041 tgaagagcca agaaaagcat tttcgccatc aaaggccatg taaaaatggt agcaattcag
    14101 attcagtcga cgactggcag ccatcatcgc cgccgcgttg accggacttg cgaaatgcag
    14161 gtcagttgcc cgagcgagca ccactttgtc gagctcacct tcggcgatgg ttttcgttgc
    14221 cagttcgatt aattgcgtcc agcccgtttt gtccggccag tgttgttctc gcgtggtggt
    14281 taaatgtaac ccaggcaagg gcttgatact caccagtgtg gcgataaatt cttttgcctg
    14341 aatcgcatcg tgctgaaggg agctttcgct gaatagcgtc agccgcagcg tggctttacc
    14401 gccacagcgt cgccattcca ggcggggtaa aagtaaattg ccctgcgacg ggtcaaaagc
    14461 attcagcccc caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc
    14521 ctggtccaac gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt catcaccatt
    14581 acgttgttgc cagtagaatt gcgggtacgt ttgctgactg gccagccagc tcaaggcatc
    14641 aaaagcgtct ttgagaggga aaggaatatc gataacccga atgccgggtg tcgccggaat
    14701 ctcttgcgac aaatggcgca gtagattttc cagcgccgta gtaagtgatt gcacgcggac
    14761 ctctccctgt taaaagcctc acattatacg gggtactaca aaaaaaatgc agtaccccgg
    14821 tgtagggagg ttttaacggc gtgccagcaa cagtcctagt accagcccaa cggccgcgcc
    14881 cacaccaatt ccttgccagg gtttttcgtg gacgtagtca tcagcacgat aaactgcctg
    14941 cttcgcccga tagtaataac tgtctgaagc ctggctaacc cgttttttca catcatccag
    15001 cgctttttct gcacgcgctt tcagctcaac atatttctga tcggcgggat cgccagagga
    15061 gcggagcacc tcttccagtg tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc
    15121 accaaactga ttagacattc tcgttctcca tttgcgtaaa acctgtcact aactatagac
    15181 aagggttgtt gattacgcct gaattacctc gcgtgccatc ccaatgtgtg ggataccatc
    15241 ttcttcatag acctccgtca cagggataaa accgaaactc tggtagaagt tttgcagatg
    15301 cgcctgcgcc ccgaggtaaa caggcttatc aggccagtga tgcgtacatg tttcgagtgt
    15361 tttactcatc agctgctgcc ccactttttc gccacgcaac gcttcactga caatcacccg
    15421 acctataacg accggctcaa gatcatcatc acttttcaga atcctcgcat atgccaccag
    15481 ttcatcattt ttccaaccga gaatatggcg attatccccc gtcaaatcat caccatcgat
    15541 atcctgataa gggcaattct gctcgacaac aaacaccgcg caacgcagtt gtaataaggc
    15601 ataaagctgg gaaaccgata attcagagtg atgcagatct tgccattcaa tcataatgct
    15661 ctccttttag atgtatgcct tatactagcc ggaatatttt ttgaaacgtg atgaactcat
    15721 ggaattaatt tttttaggta cttcggccgg tgtgccaacc cgcacgcgca atgtcacggc
    15781 aatattgctt aacctgcaac atccgaccca gagcggtctt tggttgtttg actgcggtga
    15841 aggcacccag catcagctac tgcataccgc ctttaaccct ggaaaactgg acaagatttt
    15901 tatcagtcac cttcatggcg atcatctttt tggtttaccc ggcttgctgt gcagtcgttc
    15961 tatgtcaggc attatccaac ccttaacgat ttatggtccg caaggtatcc gtgaatttgt
    16021 ggaaaccgcg ctgcggatta gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat
    16081 tggcgctggc gaaattctcg atgatggcct gcgcaaagta accgcttatc cgctggaaca
    16141 cccactggaa tgttatggct atcgtattga agaacatgat aaaccgggtg cattaaatgc
    16201 ccaggcatta aaagctgctg gcgtgccgcc tggcccactg tttcaggaat taaaagcggg
    16261 caaaacaatc acgctggaag atggaaggca gattaacggc gcagattact tagctgctcc
    16321 agtgccaggt aaagcgctcg ctattttcgg cgataccggc ccctgcgatg ccgcacttga
    16381 cctggctaaa ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa ccatggaagc
    16441 caaagccaat agtcgcggcc atagctctac acgccaggct gcgacactag cccgtgaggc
    16501 tggagtcggt aagctaatca ttacccacgt cagctcgcgc tatgatgaca aaggttgtca
    16561 gcacctgtta cgtgaatgca ggtcaatttt cccggcgact gaactagcga atgatttcac
    16621 cgtgtttaac gtttaactct gcttattatt aaaaacaggg cgaaacttgc cctgttatcg
    16681 caacccgcgc tacatatatc cacccacacg ggcatattga gcgatttttg atatttattc
    16741 atcaccttac gcactatatt cagagcaata agttgcctct ttttatcttc attcagcaca
    16801 acctttgcaa ctaaaaggag cccatgatgg ttacagttgt cagcaattat tgtcaattat
    16861 ctcaaaccca actcagtcag acatttgcag aaaaatttac tgtgaccgag gaattactgc
    16921 agtctttaaa aaaaacagcg ttatccggag atgaagaaag tattgagtta ctgcataata
    16981 ttgcgttagg ttatgatgaa tt
//
LOCUS       DAZFAH010000095        16980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFAH010000095 DAZFAH010000000
VERSION     DAZFAH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.095"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..765
                     /gene="nfeR"
                     /locus_tag="ACHJWB_002879"
                     /gene_synonym="yqjI"
     CDS             142..765
                     /gene="nfeR"
                     /locus_tag="ACHJWB_002879"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4314479.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(919..2439)
                     /gene="aer"
                     /locus_tag="ACHJWB_002880"
     CDS             complement(919..2439)
                     /gene="aer"
                     /locus_tag="ACHJWB_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4314480.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2857..4236
                     /gene="ygjG"
                     /locus_tag="ACHJWB_002881"
     CDS             2857..4236
                     /gene="ygjG"
                     /locus_tag="ACHJWB_002881"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4314481.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAALILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4278..4610)
                     /locus_tag="ACHJWB_002882"
     CDS             complement(4278..4610)
                     /locus_tag="ACHJWB_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4314482.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4829..5812
                     /gene="ebgR"
                     /locus_tag="ACHJWB_002883"
     CDS             4829..5812
                     /gene="ebgR"
                     /locus_tag="ACHJWB_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4314483.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5996..9088
                     /gene="ebgA"
                     /locus_tag="ACHJWB_002884"
     CDS             5996..9088
                     /gene="ebgA"
                     /locus_tag="ACHJWB_002884"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4314484.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALNLTGGELKVENKLWFTT
                     LNDYTLHVEVRAEGETLATQQIKLLDVAPNSEAPLQITLPQLDAREAFLNIMVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWLVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9085..9534
                     /locus_tag="ACHJWB_002885"
     CDS             9085..9534
                     /locus_tag="ACHJWB_002885"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4314485.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9597..11030
                     /locus_tag="ACHJWB_002886"
     CDS             9597..11030
                     /locus_tag="ACHJWB_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4314486.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            11164..12234
                     /gene="ygjJ"
                     /locus_tag="ACHJWB_002887"
     CDS             11164..12234
                     /gene="ygjJ"
                     /locus_tag="ACHJWB_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4314487.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            12251..14602
                     /gene="ygjK"
                     /locus_tag="ACHJWB_002888"
     CDS             12251..14602
                     /gene="ygjK"
                     /locus_tag="ACHJWB_002888"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO4314488.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14928..16946
                     /gene="fadH"
                     /locus_tag="ACHJWB_002889"
     CDS             14928..16946
                     /gene="fadH"
                     /locus_tag="ACHJWB_002889"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO4314489.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         4090 a         4561 c         4436 g         3893 t
ORIGIN      
        1 caatgctgcc ggatgcggcg tgaacgtctt atccggcctg gatgtgaggt gcttccacgt
       61 ttaaaaatca tttttgtact tgcagtaatg cccacatcag atataaatta gatatatcta
      121 atcaagcaaa aggaggctga tatgagccat catcacgaag ggtgttgtaa acatgaaggc
      181 cagccacgcc atgagggctg ctgcaaaggt gagaagtcag aacacgagca ctgcggacac
      241 ggtcaccagc atgaacacgg tcaatgctgc ggtggtcgcc acggtcgcgg cggcggtcgt
      301 cggcaacgtt tctttggtca cggtgaattg cgtctggtga ttctggatat tctctcgcgc
      361 gatgacagcc acggttacga attgattaaa gcgattgaga acctaaccca ggggaattac
      421 accccaagcc cgggcgtcat ctacccaacg ctggattttc tgcaggagca gtcgctgatt
      481 accatccgcg aagaggaagg tggtaagaag cagattgcgc tgaccgaaca aggcgcgcag
      541 tggctggaag aaaaccgcga acaggtggag atgattgaag aacgcatcaa agcgcgttgc
      601 gttggcgcgg cgctgcgcca gaacccgcaa atgaagcggg cgctggataa ttttaaagcg
      661 gtgctggatt tacgcgtcaa ccagagcgat atcagtgatg cacaaataaa aaagatcatt
      721 gcggtgatcg accgcgccgc ttttgatatt acgcaactgg attaatcgcc gcatccgcca
      781 gtggcgcggt gcaattgccg gatgcgacgc ttgacgcgcc ttatccggcc tacacccgct
      841 acacaccccg caggcctgat aagatgcgcc agcatcgcat caggcattgt gctccaaccg
      901 ccggatccgg cataccgatt aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg
      961 gtgtttcacc atcgccgaca cctgcgcact ctcttccact agctcggcat ttttctgggt
     1021 gatcaggtta agctcatcca ctgcacgggt caggctggaa agcccatcgg cctgttccag
     1081 cgttgaatgg ctaatctggg cgatcaactg ggtgacgttt ttcacctgtg ccacaatatc
     1141 ttccatcgtc cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct tatcagcact
     1201 ggcatcaatc agcttgcgaa tatcgttggc agcattagcg ctgcggctgg ctaaatgacg
     1261 cacttcccct gccaccactg caaaaccttt gccctgttcg ccggcacgcg ccgcttccac
     1321 cgccgcattc agggccagaa tattggtctg aaacgcaata tcgttaatca gcgaagtaat
     1381 ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt gtcttgatca ccgtggtcat
     1441 cgcctcccca ccctgcactg ccgcattact ggcggtgata gaaagtttat cggcagccga
     1501 cgccgtggca gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg
     1561 ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag
     1621 cgtctcactg ccatttctga cactggacac ctggcttgag acatcgttaa ttagccaacg
     1681 gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt
     1741 caggtgctca acactattac gctctccggt cgccaccttc agtgcctgac gggcgacatt
     1801 ttctatcggg cgcacaatct gccattcaaa acaagcgctt gccaacaata ccactaacac
     1861 acagaggaaa tacgtcacca ccggggcagc aacaaaccaa agcatggccg ccagcaagat
     1921 aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc
     1981 cagccagcct ttacgcacca ccaggccttt atgaatacgc ttactggtac gtccggcgtt
     2041 cagcgctttg tacagcggct ccaccgccgc aatctcttca tccgtcgccc gggtacgaat
     2101 cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg cccgcaccca
     2161 ataatggtcg ccatttttgc ggcgattttt cacgatgccg ctccagggct cccctttttt
     2221 cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac gcaccatgtt
     2281 gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa aagtgtcatt
     2341 agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat cgtccgccag
     2401 cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct gtgcaggtta
     2461 tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat ctttaaccga
     2521 ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattctt tgatttaaaa
     2581 tactttcact ctgttactat acgaaaacgt taattatctt gcccaaaaat caggcaatta
     2641 ttgccctgaa aacgtgcatt tgcgcagcaa tcatcaaatc catacccgac aaaaaccgtg
     2701 caaaataaca acaaatgtta acagatagca ttaaatattg cacaaatgat aaccgaattt
     2761 gtgtttatcc cgattttcgc gatcgcagcc ggagtggcgc aatccctgca atacttaaat
     2821 cggtatcatg tgatacgcga gcctccggag catattttga acaggttacc ttcgagcgca
     2881 tcggctttag cgtgcagcgc ccacgccctg aatctcattg aaaagcgaac gctggatcat
     2941 gaggagatga aagcacttaa ccgagaggtg attgaatact tcaaagagca tgtcaatccg
     3001 gggtttttag agtatcgcaa atctgttacc gccggcgggg attacggagc cgtagagtgg
     3061 caagcgggaa gtttaaatac gcttgtcgac acccagggac aggagtttat cgactgcctg
     3121 ggaggttttg gaattttcaa cgtggggcac cgtaatccag ttgtggtttc cgccgtacag
     3181 aatcaacttg cgaaacaacc gctgcacagc caggagctgc tcgatccgtt acgggcgatg
     3241 ttggcgaaaa cccttgctgc gctaacgccc ggtaaactga aatacagctt cttctgtaat
     3301 agcggcaccg agtccgttga agcagcgctg aagctggcga aagcttacca gtcaccgcgc
     3361 ggcaagttta cttttattgc caccagcggc gcgttccacg gtaaatcact tggcgcgctg
     3421 tcggccacgg cgaaatcgac cttccgcaaa ccgtttatgc cactgctgcc gggcttccgt
     3481 catgtgccgt ttggcaatat cgaagccatg cgcacggctc ttaacgagtg caaaaaaacc
     3541 ggtgatgatg tggctgcgct gatcctcgaa ccgattcagg gtgaaggtgg cgtaattctg
     3601 ccgccgccgg gctatctcac cgccgtacgt aagctatgcg atgagttcgg cgcactgatg
     3661 atcctcgatg aagtacaaac gggcatgggg cgcacgggca agatgttcgc ctgcgagcat
     3721 gagaacgtac agccggatat cctctgcctt gccaaagcgc tcggcggcgg cgtgatgccg
     3781 attggcgcga ccatcgccac tgaagaggtg ttttcagttc tgttcgacaa cccattcctg
     3841 cataccacca cctttggcgg caacccgctg gcctgtgcgg cggcgctggc gaccatcaat
     3901 gtgttgctgg agcagaactt accggctcag gctgagcaaa aaggcgatat gttgctggac
     3961 ggtttccgtc aactggcgcg ggaatatccc gatctggtac aggaagcgcg tggtaaaggg
     4021 atgttgatgg caattgagtt tgttgataac gaaatcggct ataactttgc cagcgagatg
     4081 ttccgccagc gcgtactggt ggccggaacg ctcaataacg ccaaaacgat ccgcattgaa
     4141 ccgccactga cactgaccat tgaacagtgt gaactggtga tcaaagcggc gcgtaaggcg
     4201 ctggcggcca tgcgagtaag tgtcgaagaa gcgtaatatc aaatcggatg gcgatacgac
     4261 gtcgccatcc gatcttttta tacgacgcgt acgcccgcag gcatcatccg ctccggcgtt
     4321 aacaacacgc tttcactgcc gtcatcggtt tccgcacaca acagcatgca ttccgacgtt
     4381 tcaccacgca ttttcgcttt ttgcagattg cataagacca ccaccgtttt ccccatcagc
     4441 tcttcttcgc tgtagtacgg caccaggctg gtcacggttt gcagtgtttt ttcccccaca
     4501 tcaacctgta cgatgtacag cttgtcggcg ttttcatggc gtttcacttc cacaatcttt
     4561 ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca cggtttccat tgtttcctcc
     4621 cttcgggtaa aattttacta aactatagca aaagtttttc tcaatcctgt aggctaaaaa
     4681 tggagaatgc aggcgtgatc acattcgtga gccgctgtgt taccgttaca gcgtcaaaga
     4741 aacgcgcttt atttactgaa aacaggtgac ccgataagca cttcctctac aatgggggcg
     4801 cacatcaggg aaagtaaaaa aggtaaacat ggcaacacta aaagacatcg caatcgaagc
     4861 tggcgtatcc ctggcgacag tatccagggt cttaaatgac gatccgacat tgaatgtgaa
     4921 agaagagacg aaacatcgca ttctcgagat cgccgaaaag ctggagtaca agaccagtag
     4981 tgcccgtaaa ctccagacag gtgcagtcaa ccaacaccat attctggcta tctacagcta
     5041 ccagcaggag ctggagatca acgatcctta ctatctggcg atccgccacg gcattgaaac
     5101 ccagtgcgaa aagctgggca tcgagctcac caactgttat gaacacagcg gcttaccaga
     5161 cattaaaaac gtcaccggta ttttaattgt cggcaaaccc acgcccgccc tgcgcgccgc
     5221 tgccagcgcg ttgaccgaca atatctgttt tatcgacttt cacgaacccg gcagcggtta
     5281 cgatgcggtg gatatcgatc tggcacgcat cagtaaagaa atcatcgact tctatatcaa
     5341 tcagggcgtt aatcgtattg gttttattgg cggtgaagat gagccaggca aagcggatat
     5401 tcgtgaggtc gcctttgcgg aatatggccg actgaaacaa gtggtacgcg aagaggatat
     5461 ctggcgcgga ggcttttcca gttcgtcggg ttatgaactg gcaaaacaga tgctggcgca
     5521 ggaagactat ccgaaggcac tgtttgttgc ttccgattcc attgctatcg gcgtactgcg
     5581 ggcaattcat gaacgaggcc tgaacatccc acaggatatt tcgcttatca gcgttaacga
     5641 tatccccacc gcgcgattta cctttccgcc gctctccacc gtgcgcatcc attccgagat
     5701 gatgggaagt cagggcgtta acctggtgta tgaaaaagcc cgcgatggtc gcgcgctgcc
     5761 gctgttagtc ttcgttccca gcaaattaaa actgcgcggc acgacccgtt aaatcccctt
     5821 acacactgtc cggcaatcgt ttttgccgga cagtgctgcc gtttattttc gtgatccagt
     5881 taaagtaaat gcatttacct gctacttttt agtaaaaatt ttactaaact ccccagcaat
     5941 tacacaaact accatcacca tgaatggttc cgatttctct ctaccgggag gccctatgaa
     6001 tcgctgggaa aacattcagc tcacccacga aaaccgactt gcgccgcgtg cgtacttttt
     6061 ttcatatgat tctgttgcgc aagcgcgtac ctttgcccgc gaaaccagca gcctgtttct
     6121 gcccttaagc ggtcagtgga atttccactt ttttgaccat ccgctgcaag taccagaagc
     6181 cttcacctct gagttaatgg ctgactgggg gcatattacc gtccccgcca tgtggcaaat
     6241 ggaaggtcac ggcaaactgc aatataccga cgaaggtttt ccgttcccca tcgatgtgcc
     6301 gtttgtcccc agcgataacc caaccggtgc ctatcaacgt attttcaccc tcagcgacgg
     6361 ctggcagggt aaacagacgc tgattaaatt tgacggcgtc gaaacctatt ttgaagtcta
     6421 tgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc ctgaccgcag agtttgacat
     6481 cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg cgcgtgatgc agtgggcgga
     6541 ctctacctac gtggaagacc aggatatgtg gtggtcagcg gggatcttcc gcgatgttta
     6601 tctggtcgga aaacacctaa cgcatattaa cgatttcact gtgcgtaccg actttgacga
     6661 agcctattgc gatgccacgc tttcctgcga agtggtgctg gaaaatctcg ccgcctcccc
     6721 tgtcgtcacg acgctggaat ataccctgtt tgatggcgaa cgcgtggtgc acagcagcgc
     6781 cattgatcat ttggcaattg aaaaactgac cagcgccagc tttgctttta ctgtcgaaca
     6841 gccgcagcaa tggtcagcag aatcccctta tctttaccat ctggtcatga cgctgaaaga
     6901 cgccaacggc aacgttctgg aagtggtgcc acaacgcgtt ggcttccgtg atatcaaagt
     6961 gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg ctgcacggcg tcaaccgtca
     7021 cgacaacgat catcgcaaag gccgcgccgt tggaatggat cgcgtcgaga aagatctcca
     7081 gttgatgaag cagcacaata tcaactccgt gcgtaccgct cactacccga acgatccgcg
     7141 tttttacgaa ctgtgtgata tctacggcct gtttgtgatg gcggaaaccg acgtcgaatc
     7201 gcacggcttt gctaatgtcg gcgatatcag ccgtattacc gacgatccgc agtgggaaaa
     7261 agtctacgtc gagcgcattg ttcgccatat tcacgcgcag aaaaaccatc cgtcgatcat
     7321 catctggtcg ctgggcaatg aatccggcta tggctgtaac atccgcgcga tgtaccacgc
     7381 agcgaaggcg ctggatgaca cgcgactggt gcattacgaa gaagatcgcg atgctgaagt
     7441 ggtcgatatt atttccacca tgtacacccg cgtgccgctg atgaatgagt ttggtgaata
     7501 cccgcatccg aagccgcgca tcatctgtga atatgctcat gcgatgggga acggaccggg
     7561 cgggctgacg gagtaccaga acgtcttcta taagcacgat tgtattcagg gacattatgt
     7621 ctgggagtgg tgcgaccacg ggatccaggc gcaggatgac aacggcaatg tctggtataa
     7681 attcggcggc gactacggcg actatcccaa caactataac ttctgtcttg atggtttgat
     7741 ctattccgat cagacgccgg gaccgggcct gaaagagtac aaacaggtta tcgcgccggt
     7801 aaaaatccac gcgctaaatc tgactggcgg cgagttgaaa gtcgaaaata aactatggtt
     7861 taccacgctt aatgactaca ccctacacgt agaggtgcgc gccgaaggtg aaacgctcgc
     7921 gacgcagcag attaaactgc tcgacgttgc gccgaacagc gaagccccct tgcagatcac
     7981 gctgccgcaa ctggacgccc gcgaagcgtt cctcaacatt atggtgacca aagattcccg
     8041 cacccgctac agcgaagccg gacaccctat agccacttat cagttcccgc tgaaggaaaa
     8101 caccgcgcaa ccagtgcctt tcgcaccaaa taatgcgcgt ccgctgacgc tggaagacga
     8161 tcgtttgagc tgcaccgttc gcggctacaa cttcgcgatc accttctcaa aaatgagtgg
     8221 caaaccgaca tcctggctgg taaatggcga gtcgctgctg acccgcgagc caaagatcaa
     8281 cttcttcaag ccgatgatcg acaaccacaa gcaggagtat gaagggttgt ggcagccgaa
     8341 tcatttgcag atcatgcagg aacatctgcg cgattttgcc gtagaacaga gcgatggtga
     8401 agtgttgatc atcagccgca cagttattgc accgccggtg tttgacttcg ggatgcgctg
     8461 tacctacatc tggcgcatcg ctgccgatgg ccaggttaac gtggcgcttt ccggcgagcg
     8521 ttacggcgac tatccgcaca tcattccgtg catcggtttc accatgggga ttaacggcga
     8581 atacgatcag gtggcgtatt acggtcgtgg accgggcgaa aactacgccg acagccagca
     8641 ggctaacatc atcgatatct ggcgcagcac cgtcgatgcc atgttcgaga actatccctt
     8701 cccgcagaac aacggtaacc gtcagcatgt ccgctggacg gcactgacta accgccacgg
     8761 taacggtctg ctggtggttc cgcagcgccc aattaacttc agcgcctggc actataccca
     8821 ggaaaacatc cacgctgccc agcactgtaa cgagctgcag cgcagtgatg acatcaccct
     8881 gaacctcgat caccagctgc ttggcctcgg ctccaactcc tggggcagcg aggtgctgga
     8941 ctcctggcgc gtctggttcc gtgacttcag ctacggcttt acgttgctgc cggtttctgg
     9001 cggagaagct accgcgcaaa gcctggcgtc gtatgagttc ggcgcagggt tcttttccac
     9061 gaatttgcac agcgagaata agcaatgagg atcatcgata acttagaaca gttccgccag
     9121 atttacgcct ctggcaagaa gtggcaacgc tgcgttgaag cgattgaaaa tatcgacaac
     9181 attcagcctg gcgtcgccca ctccatcggt gactcattga cttaccgcgt ggagacagac
     9241 tccgcgaccg atgcgctatt taccgggcat cgacgctatt ttgaagtgca ttactacctg
     9301 caagggcagc aaaaaattga atatgcaccg aaagagacat tacaggtagt ggaatattat
     9361 cgtgatgaaa ctgaccgtga atatttaaaa ggctgcggag aaaccgttga ggtccacgaa
     9421 gggcaaatcg ttatttgcga tatccatgaa gcgtatcggt ttatctgcaa taacgcggtc
     9481 aaaaaagtgg ttctcaaagt caccatcgaa gatggttatt tccataacaa ataacaacta
     9541 cggcggcaaa aggagtttgc cgccaccgct accctacaca ttttcggaga tgtgttatgt
     9601 ctgataccaa acgtaataca atcggcaaat tcggcttgct ctcgctgact tttgccgccg
     9661 tttacagctt taacaacgtt atcaataata atattgagct tggactggcc tcggcaccga
     9721 tgtttttcct cgcgacgatt ttttatttta ttcccttctg tctgatcatc gcagaatttg
     9781 tttcgttaaa taaaaactca gaagccggtg tctacgcgtg ggtaaaaagt tcgctgggcg
     9841 gacgttgggc atttattact gcctatacct actggttcgt aaacctgttc tttttcacct
     9901 cactgttgcc gcgcgttatt gcttatgctt cgtatgcctt cctcggatac gaatatatta
     9961 tgacgccggt tgccaccacc attatcagta tggtgctgtt cgccttctcc acctgggttt
    10021 ccaccaacgg ggcgaaaatg ttggggccaa ttacctccgt cacttcaacg ctgatgctgc
    10081 tgttaacgct ctcctacatt ttactggcag gtacggcgct ggttggcggc gtacagcctg
    10141 ctgaccccat caccgttgac gcgatgatcc cgaacttcaa ctgggcgttc ctcggcgtta
    10201 ccacctggat ctttatggcc gcaggtggcg cggagtccgt cgctgtgtac gttaacgacg
    10261 tcaaaggcgg ttcgaaatcg ttcgttaaag tgatcatcct cgccgggatt tttatcggcg
    10321 tactgtattc cgtctcctcg gtgctgatta acgtcttcgt cagcagcaaa gagttgaaat
    10381 ttaccggcgg atcggtgcag gtattccacg gcatggcggc gtattttggt ctaccggaag
    10441 cgttgatgaa tcgctttgtc ggtctggtgt cctttaccgc gatgttcggt tccctgctga
    10501 tgtggaccgc aacgccggtg aaaattttct tctccgaaat cccggaaggc atctttggta
    10561 agaaaaccgt cgaactgaac gaaaacggcg ttccggcgcg cgcagcgtgg atccagttcc
    10621 tgatagtcat cccgctgatg attatcccga tgctcggttc caataccgtg caggatctga
    10681 tgaatactat tattaatatg accgccgcag cgtccatgct tccgccgtta ttcatcatgc
    10741 tggcttacct gaatttacgc gccaaattag atcacctgcc acgcgatttc cgtatgggct
    10801 cccgccgcac cggtattatc gttgtttcaa tgctgattgc gatatttgcc gtagggtttg
    10861 tcgcttcgac attcccgact ggcgcgaata ttctgaccat cattttttat aacgtcggcg
    10921 gtattgttat cttccttggc tttgcgtggt ggaaatacag taaatatata aagggattaa
    10981 cggctgaaga gcgccatatt gaagcgacgc cagccagcaa tgttgattaa gcagaataat
    11041 aaaaaatgag caggcatttt ccctcttcct ggatgaggag agggaaaata gtttctgcct
    11101 ttatattttt ataattacaa cgataaaagg ctgtactttt tctttagctc atggattaac
    11161 acaatgaaat taatcactgc accatgcaga gcattacttg ctctgccgtt ttgctacgcc
    11221 ttttctgcgg caggagaaga agcacgtccg gcagaacatg acgacacaaa aacacctgca
    11281 attacctcga catcttctcc ttcatttcgt ttttacggcg aattaggggt tggtggatat
    11341 atggatttag agggtgagaa taaacataaa tacagcgacg gtacctatat tgaaggtggc
    11401 ctggagatga agtacggctc ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag
    11461 gccgaccacg acggcaatgc ctgggtgcca gaccatagct ggggtggttt cgagggcgga
    11521 attaaccgtt tctatggcgg ttatcgtacc aatgatggca ccgaaatcat gctcagtctg
    11581 cgtcaggatt cctcgctgga tgacctgcaa tggtggggcg acttcacccc cgatctgggc
    11641 tacgtcattc ccaatacccg cgacattatg accgcgctga aggtgcagaa cttaagcggc
    11701 aactttcgtt atagcgtcac cgccacacct gccggacatc atgacgaaag caaagcctgg
    11761 ctacattttg gcaagtacga tcgctatgac gacaaataca cctatccggc aatgatgaac
    11821 ggttacatcc agtatgacct tgccgaaggc atcacctgga tgaacggtct ggaaatcacc
    11881 gacggcacag gacagctcta tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc
    11941 tggcaccata ccggacgcgc cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg
    12001 gtgagcgcca tgtatgaagc gttaaagggc gtttatctct ccaccgctta cacctacgcc
    12061 aaacatcgcc ctgaccacgc tgacgatgaa accacctctt tcatgcagtt tggtatctgg
    12121 tacgaatacg gcggcggacg tttcgccacg gcttttgata gccgcttcta catgaaaaat
    12181 gcctctcacg atcccagcga ccaaatcttc ctgatgcaat atttctactg gtaataagga
    12241 ctgtaatttc atgaaaataa aaaccatttt aacgccagta acctgcgctc tgctgataag
    12301 tttttccgcc catgccgcta acgccgacaa ttataaaaac gtgattaacc gtactggcgc
    12361 gccgcagtac atgaaggatt acgattacga cgatcaccag cgttttaatc cgtttttcga
    12421 tctcggagcc tggcatggtc atctgttgcc agacggccct aacaccatgg gcggctttcc
    12481 gggcgttgcg ctgctgacgg aagagtacat caactttatg gccagcaatt tcgaccgcct
    12541 gaccgtctgg caggacggca agaaagtcga ctttacgctg gaggcataca gtattcccgg
    12601 tgcgctggtg caaaaactga cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc
    12661 cacgccgcgc acgtcactac tggaaaccaa aatcaccagc aataaaccgc tggatctggt
    12721 gtgggacggc gaactgctgg aaaaactgga agcgaaagaa gggaaaccgc tttccgataa
    12781 aaccattgct ggcgaatacc ctgactatca gcgcaaaatc agcgccaccc gtgatggcct
    12841 gaaagtcacc tttggcaaag tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga
    12901 atatcaggtg cataaatccc tgccggtgca gactgaaatc aacggtaatc gctttaccag
    12961 taaggcgcat atcaacggtt cgaccacgct ctacaccacc tattcccatc tgctgaccgc
    13021 tcaggaagtt agcaaagagc aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta
    13081 tctcaccgcc tcgcagcaac gctgggaaga atacctgaag aaagggttaa ccaatccgga
    13141 tgcgacgccg gaacagacgc gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg
    13201 gcgctcaccg ggcggtgcgg tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg
    13261 gttctccggc aatcagacct ggccgtggga tacctggaag caggcgtttg cgatggcgca
    13321 tttcaatccg gacatcgcca aagagaatat ccgcgcggtc ttctcctggc agatccagcc
    13381 tggcgatagc gtgcgtccgc aggatgtggg ctttgtcccc gacctgatag cgtggaacct
    13441 tagccccgag cgtggtggcg atggcggcaa ctggaacgaa cgtaatacca aacccagcct
    13501 tgccgcctgg tcggtgatgg aagtgtacaa cgtcacccag gataaagcct ggttggcaga
    13561 gatgtacccg aaactggtgg cctatcacga ctggtggtta cgtaaccgcg atcacaacgg
    13621 caacggcgtg ccggaatatg gcgcgacccg cgacaaagcc cacaacactg agagcggcga
    13681 gatgctgttt acggtgaaga aaggcaacaa agaagagacg cagtctgggc tgaacaacta
    13741 cgcccgcgtg gtggagaaag gccagtacga cagtctggaa attccggcac aggtcgctgc
    13801 gtcgtgggaa tcggggcgtg atgatgccgc cgtctttggt tttatcgaca aagaacagtt
    13861 ggataaatat gtcgctaatg gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa
    13921 ccgcagtcag gacggaacgt tgctgggcta ctcgctattg caggagtcgg tggatcaggc
    13981 cagctatatg tacagcgata accattatct ggcggagatg gcaaccatcc tcggtaagcc
    14041 agaagaggcc aaacgctatc gccagttggc acagcagctc gcggactaca tcaacacctg
    14101 tatgttcgac ccgactaccc agttctacta tgacgtacgt attgaagata aaccactggc
    14161 gaacggctgc gcgggcaaac cgattgttga gcgcggtaaa gggccggaag gctggtcgcc
    14221 gctgtttaac ggtgcggcaa cgcaggccaa tgccgacgcg gtggtgaagg tgatgctcga
    14281 tcctaaagag ttcaacacct ttgtcccgct gggaacggcg gcgttaacca atccggcttt
    14341 tggcgctgat atctactggc gtgggcgcgt atgggtggat cagttctggt ttggtctgaa
    14401 agggatggag cgttacggtt atcgcgatga tgccctgaaa ctggcggata cgttcttccg
    14461 tcacgccaaa ggattaaccg ccgatggccc aattcaggag aattacaacc cgctgactgg
    14521 cgcacagcaa ggcgcaccaa atttctcctg gagtgccgcg catttgtata tgttgtataa
    14581 cgattttttc cgtaagcaat aatttgcaac tgctggcgga tgcggcgtaa acgccttatc
    14641 cgccctacat gtgcaatcct cctggccgga taaaacgcga caagcgtcgc atccggcatc
    14701 tgcaccgtgc cactggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
    14761 ccaggccgga taaaacgcgg caagcgtcgc atccggcgtt accaccgggc gtattctttt
    14821 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccggcgac ggctaaatta
    14881 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctacccgt
    14941 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
    15001 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
    15061 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
    15121 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
    15181 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
    15241 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
    15301 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
    15361 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
    15421 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
    15481 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
    15541 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
    15601 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
    15661 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
    15721 cgcgcggcgc atttacttgg gtcacgcgca aactgaaagg ccacgtttcg ctgccgctgg
    15781 tgaccaccaa ccggattaac gatccgcagg ttgccgacga cattctctcg cgcggcgatg
    15841 ccgatatggt gtcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
    15901 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
    15961 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgt cacgaaacca
    16021 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
    16081 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
    16141 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
    16201 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
    16261 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
    16321 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
    16381 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
    16441 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
    16501 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
    16561 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
    16621 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
    16681 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
    16741 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
    16801 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
    16861 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
    16921 gaacacggct ggcgctggag atttaaatcg ttatttgaag cgccggatgc aacgcatccg
//
LOCUS       DAZFAH010000096        16724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFAH010000096 DAZFAH010000000
VERSION     DAZFAH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.096"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..563)
                     /locus_tag="ACHJWB_002890"
     CDS             complement(<1..563)
                     /locus_tag="ACHJWB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO4314490.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLN"
     gene            complement(781..1653)
                     /locus_tag="ACHJWB_002891"
     CDS             complement(781..1653)
                     /locus_tag="ACHJWB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4314491.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVMQADKTEPC
                     HEWDMAGIQPSPAEAQNIREKTEAVFRLFRPVHRVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            complement(1737..2888)
                     /locus_tag="ACHJWB_002892"
     CDS             complement(1737..2888)
                     /locus_tag="ACHJWB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314492.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTPSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            complement(4156..4452)
                     /locus_tag="ACHJWB_002893"
                     /pseudo
     CDS             complement(4156..4452)
                     /locus_tag="ACHJWB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(5282..5884)
                     /locus_tag="ACHJWB_002894"
     CDS             complement(5282..5884)
                     /locus_tag="ACHJWB_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314493.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(5978..6184)
                     /locus_tag="ACHJWB_002895"
     CDS             complement(5978..6184)
                     /locus_tag="ACHJWB_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4314494.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(7317..7511)
                     /locus_tag="ACHJWB_002896"
     CDS             complement(7317..7511)
                     /locus_tag="ACHJWB_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314495.1"
                     /translation="MVIRKKKCRDCGNAITHNTCCPYCGSVAPFGYYRNTDRIVTILL
                     ALIIVVLLTTVSVSQCVYTV"
     gene            7710..8279
                     /locus_tag="ACHJWB_002897"
     CDS             7710..8279
                     /locus_tag="ACHJWB_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4314496.1"
                     /translation="MTYKYNSFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLVRDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            8539..8940
                     /locus_tag="ACHJWB_002898"
     CDS             8539..8940
                     /locus_tag="ACHJWB_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4314497.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPGALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            8928..9338
                     /locus_tag="ACHJWB_002899"
     CDS             8928..9338
                     /locus_tag="ACHJWB_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO4314498.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVKKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            9622..11241
                     /locus_tag="ACHJWB_002900"
                     /pseudo
     CDS             9622..11241
                     /locus_tag="ACHJWB_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            11321..11613
                     /locus_tag="ACHJWB_002901"
                     /pseudo
     CDS             11321..11613
                     /locus_tag="ACHJWB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(11653..12687)
                     /locus_tag="ACHJWB_002902"
     CDS             complement(11653..12687)
                     /locus_tag="ACHJWB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO4314499.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIIDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(12690..13655)
                     /locus_tag="ACHJWB_002903"
                     /pseudo
     CDS             complement(12690..13655)
                     /locus_tag="ACHJWB_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(13712..14470)
                     /locus_tag="ACHJWB_002904"
     CDS             complement(13712..14470)
                     /locus_tag="ACHJWB_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO4314500.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(14484..15698)
                     /locus_tag="ACHJWB_002905"
     CDS             complement(14484..15698)
                     /locus_tag="ACHJWB_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO4314501.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGSLASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(15894..16232)
                     /locus_tag="ACHJWB_002906"
                     /pseudo
     CDS             complement(15894..16232)
                     /locus_tag="ACHJWB_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4184 a         3978 c         4199 g         4363 t
ORIGIN      
        1 tcgtttaacg atgccagtcc ggtaaagatt tcctcctccc cgtggtattc atttttgagc
       61 cagccatcat ccgtcagaat atccagcagt tcgcgggtat ccccggtttg ctgcagggtc
      121 tgtgcgacct gctgtcctgc cggggtggcg agtttttcct gcagtgtgat taataaattg
      181 cggacaggct gaatatgttc cagatggaga accggctcag tttttccggg aacgtggcgg
      241 atgagcgtcg gtacagaggt catggggcgg ttacggttag gcagaatttc ctgccccaca
      301 atggcgttga tggtggttga tttgcctgct ttcatcgtgc cgacaacagc cagcaccatc
      361 tccctgcggg cggttttgat ttgttcgccc tccagctcct caattctttt cagtgctttc
      421 tgtttatcaa aaagcagtct gttttcgttc ttactgtcag ataacacatc tggctcctca
      481 accatttgcc ggagcagatt aatgttcagt tgcaaaagtc ggtcggcttc atcacaaagc
      541 agggcgatgt tcttttcgtg cattatttaa ccttcctaag acgcattttc atgacaacca
      601 cggaggaggt catttactac aaacgatcgt ttataacgat cgatatgata gtaacgatcg
      661 atgtattcta ttagcttgag aaacacaacg caaggggaca acccgggtat ggctgcgttt
      721 gcatagtgtt ttcatggagt taaaaatctg taacaacagc ctggtacatc accaccgggt
      781 tcagaagaaa atccagttcc ataccgcgcg ggcaacagag accaccgagt cacggacggc
      841 gcgcagaacc gtgcgtgcaa cagaggcaat gcagacgctc tccgccgtgt caaatatccg
      901 gtccaccgca ccggtaaact gttcacgggc ctgagaccgg acagactccg tgcgcagctc
      961 gtcctgcagg cgggtcatca ggggactggc ggcatggtcg ggaagtgctg tcatgagcgc
     1021 actgaccagc gtatccagtt cccagccggt gcgggccgat acggcaacaa cccgatgtac
     1081 gggccggaac agacggaata ccgcctccgt tttttcgcga atgttctgtg cctctgcggg
     1141 agaaggctga ataccggcca tatcccattc atggcagggc tccgttttat cggcctgcat
     1201 caccacaaac agtacccgct gatgtccccg gtgcaggatg tgtcgccaga aatactcatc
     1261 cacagacagg gcacggtcat cggctttaat cagccacagt accaggtcca gttcgagcag
     1321 aatgtcacgg tacagggctt catactctgc atccctgtcc cggctctcgc ccaccccggg
     1381 caggtcagtg ataaccatgc tgtgaccatg gccattcaga cggaagcgct gcacttcccg
     1441 ggtgccggcg tgaacatcac tgaccggggt gacctccccc tgaaacagcg cattacagag
     1501 tgaggattta ccggccccgc ttttacccat aatgccaggc acgggttcgt gactggtgag
     1561 tttgcgcaga tgttccagga tgtgacggga aagtgagtaa ggcagggagg agagcggttt
     1621 ttcaattgcc tcaatggcat cagacggatt catacatgta ttcccggtga aaaaagacaa
     1681 aaccccgcgc accggaaagg tggcggggga ttcacggaaa taatatctgt ctgaaattat
     1741 ctggaatcga tttggctgtt ctgcctggtt gccaccaaat catggtacgc ctgctcaatc
     1801 atttcattca gcagtttttt tgggaggaca ccacgacttc gggcaatact ttcaagctgt
     1861 ttgtggattt ttccggacag tttcagaggg acggaaccat cagcacgttc atcacgcctt
     1921 ttttgttgct cccgggcact tttcagctca tcaagaagaa tcgctttatg ctgaatcctg
     1981 ggggcctgga aggagacctg cgtaaacttc gctctgttcc gtctgggatt actttccagt
     2041 aaaaactgta cctgttgctc attccagccc tcccagttat caaacgtggc gcaggcaaac
     2101 cagtatttct gctccggggt aaggggattc agcggggttc ccacacaccg gacctgcatg
     2161 gcgttccata accagtcaca ctgttcactg tccctgctgt ccacccacga aaagggggag
     2221 gggtaatcat tgtttatgct ggcccattca gagaatactg agttgataat cgtcctcgta
     2281 gcctctggtc cggatttcag caggtaactg gagcagaacc atataatttc acggtagatt
     2341 ttatccggat gcacggtgcg catcggcttt tctctgggac cggcaatctt cggttgcgat
     2401 ggaaaccccg tggcaaaccg cagatgttcc gggggcattc ctgaacgttt ttcaggaatg
     2461 ggtgtgagtc ctgcgtttct ggtcttttcc gcttgtccgg tattgcctcc ggccggagtg
     2521 ttgtcgggag tatcttccgg aggcgaggtg ttttcattag ttgggggaac aacagcagaa
     2581 ctgtccatct ccggaatggg ttgaggggca taatcactgt ggtagccagg ggaaggcgtt
     2641 aagtcccgaa gggtgacatc aggttccggg cagtgagtgc caccgggagt gaacgggagt
     2701 tgttgctttt gggagaaaaa tgtattgtga atgcgggtat acagctggaa cagaaaaaaa
     2761 taagatgccc gtggttcttt ttttaaccac tgtgtatatt cctctatggc accgagctgt
     2821 tgtttgtgcc gttttacagc atcgatatac ggacagctgg tgcagatatc atccagttcc
     2881 tttcccacaa tacacctcac ctgcttttct cacatttgca gattttaaaa aagcaaacaa
     2941 aaacctccgt acatgatgta cagaacaatc taccaggtag tggcgtcaaa aaacctccgg
     3001 gccgcctgca ctttccggaa tgcgccggtt atgaaaaacc ggaaattcag tctctgttgt
     3061 aagatgtacc ctctgatact acgttttctc tgtgtattta actcactgta tttattgtct
     3121 ttttaagctt gactgatatt ctccggtcat agtgtgccgg atatacatcc cgtaaacttc
     3181 tcatttttat ataaaaatac ctgcactctc ttcagcgcat ctgtctcctt taagtaaaaa
     3241 atgtttttac gtattgctgt aaattcattt taaatcagta tattaccttg atattgctgt
     3301 ctcctggttt gatgcgtaat cgttctgcct ttctggatga ttctggtgct tccgggtgca
     3361 ttctcctctg cacatgcaac agttcgtcat cgccacgctc ccaaaaagca tataaaaacg
     3421 aatcgaattt gatatttttt tcaatcgttt ttcataggaa tttcgtaggg tattctccgc
     3481 tataacctcc atatttgctg tctttttctt cataaatcgt atgcaatgta gcctgtctgt
     3541 caagacctct tctctgctgt tgaggagacg atctcatttt tggtcttttc cgggagagag
     3601 gctgtcagat gagcgagccg gatatactgt cagaggtaca ccccgtagca gacctgtgct
     3661 cccatttccg cgatccggaa ccaacaacac cctacggtta ataccatttg aagcgtcagg
     3721 tactgcagga agttcccccc tgcgcggaca tcggttcctg ccgacatctc acaatacggc
     3781 cagactgtca tgcagtaaca cccgcatgcg gtctgcggtt ttaagcgcac tctcccgttt
     3841 tcagtgcacc gataatgcgt catagtaagt ctgggggaag ttacggaatg cctgttaagt
     3901 ggtgccgtgg gtggagacag cttacttttc tacagcaact gcagtcattc aggatgatca
     3961 ggagatattc tgtcaatgtc acgatcctgc cattttcgta cccgctaaga tacttccgtt
     4021 actgatgctg tccgcttttc accatttcat cctcactgac aggcgttgcg ttttgggtca
     4081 tcaccacagc tttacgtttc tcttttaacc ggtaacgctc tcctttgata ttcagcgtgg
     4141 ttgagtgatg cagcagtcaa tccaggagcg ctgttgtccg gcaaccacca tatcaacttc
     4201 ttctcgggtg tggcggagag gtggtatcct ggctgagttt cgaagcgaac cgttcttttt
     4261 gacgggcgca ttttacgtcc gggctgaatg tagtaacgcg gcatggaacg tcggcccgta
     4321 taccattgcc ttaatctccg caaagataac ctcgtcattc cggacattct ctgccaggca
     4381 tatgtcgatg taatccataa acgatttcag cttacccctt ttgtggccag tctttctggc
     4441 tggcggttca ggggatgttt tctggttatc gtgtaccaca aagcagggat attgccccga
     4501 tagtcacatt tcagattgat cgcggtctga agatgtggca ctgaggtaac gtgctggtgt
     4561 cactggtatt gctgaagtta tggtttttcg gggtcaggtg agcctgttgt gtgtatgctt
     4621 tttgttcagg tcagaggggg actgtgtgga cagcggcttt tgtctccggg cattcacgcc
     4681 tgaacgatga atgcgatttt cagcaatatt tgtctttgtg acactctctg gttgagaagc
     4741 taatgcgcgc ccctgtttcc acagagaatg aggcttgcca tcagccgaaa catcagtatt
     4801 ccacgccata cagacggcgc ctgaccatgt gctgatgcag gtaaaatgtt tcactacaac
     4861 tgttcgggtt gctatgcaac gaaacagcga ttctcacgat ctttaactgc aacgtttttc
     4921 cgcctgaaag ctgactgcct cctgctgagg tgggaggcta tgattcagtg cgtttatagt
     4981 ctccggggta tgcatgtacc ggagcatacc atttctggtg gataacaagc cggcacattc
     5041 agactgcagt gaagatggag gcacctggtt gtgaagaacg tttctgcccg tggctgcttc
     5101 gcatcatcaa tggtattcat acccgctcag aaatgaccgg gtgctgaagc cctccgttat
     5161 cgcgcagtat aatgacgtat atgatctgtg gacgagtgag cggcgtgcct gatgtacgcc
     5221 gctattactg tattttacca cagggctggt tttctgctgc cagccctgtg gttctcagac
     5281 atcaactgtg agcggcatca accgatgtac ggtatgccat aagcacgtac tgatccatca
     5341 gatttgtcag tggttcactc tgaccctgac gtgcaatgat ccattgctcc agatcttttt
     5401 ctgatttgtg tttcaggttt ttcgtccagt cgtcaaggct gtttccggtc attaagtcaa
     5461 gccagcagag ccagaccagt ttgagacgta actccgtgcc gccaaactga cggaagaggc
     5521 tgatcagttt gtttgccaga gaatgttgct tcaggcgctc acattcagac agcaggatcc
     5581 cttccagcat cggatgatga accagcaggt gttcgctggt gcataaattc accaccagat
     5641 catcatccag ggacggggag ctccgtttgt ccggaccata aatcagccgg tgcagactct
     5701 ggggaacgat ggcaatctcc ggtttttcgc cattgaatga cacccacccg ggcgtcagat
     5761 tatggaaata gtagatattc ggatgacaca cgccccatga tacggcgtgc cgggtgaagc
     5821 cggtcagcca tttctggtag ttttcatgga gcagctcgta acgggtctga tactgttgtt
     5881 tcattttcat attcctcacg ggggataaca ggcagcagag cgccgccggc aggccggaga
     5941 ttgcgtctgc tgtgggcgaa gggttagtgg ttaaaaatta cggacgggac tgtgcaatac
     6001 gcgcctgcag ccaggcttcc acttcacttt tcagccagcg ggagctgcgc cccattttga
     6061 tgggcgcagg aaagccccca tctttgatga gtttgtcaaa ccacttatcg gttaggccgg
     6121 tcagttgagt gataaacgcc atgtcgacca tctggtcatc catcagcgaa actggggtag
     6181 ccatgagaat gttcctccgg atgttgttaa accagccggt gtggtcatga aaacagcgcg
     6241 ccaccttcac cggcataaca accggggaga aacgtaaaaa ataatcggga atgtggctgt
     6301 ccggagataa acggggggat tgcgatggca gagccgtagc ggaatcccgg gggggttgtt
     6361 gcgaaaagat gcttccgcac gcacgcgcga taaagagggg atagttttcg tgaggacagg
     6421 gagagaagca ggcgttctga taattgcaga gttgttttcg gaggtggtta cagtgagtca
     6481 gcccgttcct ggcgagcagg agactgatat caccgatggg ataaggttgt ctgattggta
     6541 ttataaatat aagaagtcat cagagagtaa cgtaaaggat ttgccagaat aacctaagag
     6601 agtaaatctc tccggtaaca gagcgctgtg aagcattctg tatcagagtg cagatacatg
     6661 aacgctgatg cgtaaaatca gcagtgacac gccagctccc cccatgccca ttcctgtata
     6721 agacatgaac tgagcgtatt aatgcttctg ccctgagact gagccggtga gtgaggggct
     6781 gagaaatccc tcttgacaca gctctaacca gaaatacttc cccggttgtt gcggtgcgtc
     6841 ctgatccccg ataacggaac actaaggctg tattgcggga caccgggtaa gtggcagccc
     6901 gtaaggcgtt ctgtcaacgc cagaaaaaat gatttcccac acgcgttata gactcactcc
     6961 tgtggacctg gaagcccgta actctgcggt tgcccactga ctgacggaac tgaccgcaaa
     7021 ttttacgagt gcgtgctggg cttcagtctg gtcacctgag cgttcggtaa cggaggcgcg
     7081 ggcggtaata ggctgatttt caggtattac cggcttacag cgtgtggttg atattgaggg
     7141 gctgtgagtt tcgcgtattt gtcagaaacc cagacgagtt ttcctgtatg ggacgaaata
     7201 aggcagcggc aatacctgga aaaatttaat atggtatgaa ggagaacctg tcggttacgc
     7261 ttaatatacc cggtacactg tcagctcagg ggatacatga accgttatct caccagctac
     7321 acagtatata cacactgact gacgctgaca gtagtcagca ggaccacaat aatcagtgcc
     7381 agcagtatgg taacgatacg gtctgtattc cggtaatagc cgaagggggc gacagaacca
     7441 caatacgggc aacatgtatt atgagtaatc gcgtttccac agtccctgca cttctttttg
     7501 cgtatcacca tcactccctc ctcacagcct tacccgtaac cggtttttta cattaaaaaa
     7561 acaacgctga aaattcaacc gtcagtccgg agacgatagt tggggtatca cagagtgcct
     7621 gagacagtgt cctgccggag gtcacttcag ggactctgcg cattttttac gacgtggcta
     7681 ttccgtatga agcatacgga gatttaacca tgacctacaa atacaactcc ttctggcagc
     7741 aacgtattcg tgagacggtg cggcacgcac tgaatgttca tccccgcctg acggcattgc
     7801 gggttgacct gcgtttcccg gatgtaccgg cagcaacgga cgcagctgtg atatcccgct
     7861 tcatcaatgc cctgaaagcc cgaatcgacg cttaccagaa acgtaagcat cgggaaggta
     7921 aacgcgtgca tcccacaacc ctgcattacg tctgggcccg ggagtttggg gagtgcaaag
     7981 gtaaaaaaca ctatcacctg atgctgctgg ttaaccggga tacctggtgt cgtgccggtg
     8041 attaccgtgc tccgggatca ctggccggga tgattaaaca ggcgtggtgc agtgccctgg
     8101 gagtggatgt cgggtgccat gccacgctgg tgcattttcc ggcctggccg gcggtgtggc
     8161 tggtacgcga tgatgacact ggctttcagc aggtgctgga acgtgctgac tatctggcga
     8221 aggaacatac caaagctcac tgcaccggtg agcgcaattt tggctgtagc cggagttgag
     8281 tacgactggc gcgccaatat ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg
     8341 atgtatccac tcgcccactg actgtttttt acgtcagtgt tccattgtgt gacaaccaca
     8401 gtgctgtaac agcaaccagc ctgctttacc caaactccag tccagatttt aactgaaccg
     8461 ccattcacac cctgaccacg ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg
     8521 cggctgagga atatggctat gtacgcaaaa tcctttatcg ctcttgatgg caacggacgt
     8581 ctgacgggcg cccgtactgc acaggccgca ccttatgcta actacacctg ccacttgtgt
     8641 ggcagtgcac tcagatacca tccgcaatac gacactgaac ttccctggtt tgaacacact
     8701 gacgacaggc tgacagagca cggtcaacag tgcccttatg tcaggccgga gcgcagagaa
     8761 atacagttga ttaaacgtct gcagcaattc gtaccgggtg ccttacccgt ggtgcgtaaa
     8821 gccagctggc actgcagaca atgtcaccac gattattatg gggagcggta ctgcacacac
     8881 tgccagaccg gaggattcag tattccccgg acaactcagg aggaaatatg cgaattctga
     8941 actgttatat ggcgaatgac agcaaaggcc attttgttac ggcgaaagaa gcagcgaagc
     9001 acaaccgcca ggacgttttg tgctgtgtgt cctgtggatg cccgttaaca cttcagcggg
     9061 gcaatgacgg acaaccaccg tggtttgaac atgaccagat gactgtcgct gagaaaatcc
     9121 tgctgcgatg cacctggctt gacccggcag agaaagaggc ccgtcgtttg catctgcagg
     9181 gtatgacggt tccggattat acggtgaagg tgaaaaagtg gttttgtgtg atgtgtgacg
     9241 aagattatga aggagaaaag tgctgcccgc gttgtggtac cggggtatac agcagggagg
     9301 gggggctgca ggaaggcaac tggaaggata ggaactgagc ggttcagtcg cggaaatcag
     9361 ttgcagagct gtaatgatat ctccgggagt gctgcgttgg taggggcatc ccgggataga
     9421 gaaattttag cactttgccc tgaatgattg tagacgttcc gagtactcgt accggtctga
     9481 gttattacag ttagatattc actttttctc ccgcatactt cacatctgtc aacgacggat
     9541 gaaaagtggt tcttccgcaa cctgggtgga agaaaatgga tgaaactttc tggcgaacaa
     9601 ggatctaagc tgttttaagt tatgggcaac gcaatgcact ctcttaagac acttctacag
     9661 ttaccttgcg gatggcgatg cagtcgacaa attattagct ctgacggtat aaccctccat
     9721 ctccacggaa aacgcaaaac agcacaatgt cctgaatgct ttaagcgtag cgactctgtt
     9781 catagttgtc gtcggcgccg gatacagcat ctaccctgct cagggcagac gctatggctt
     9841 gtattttccg tccgccactg gtactgccgt aacccggctt gttcacgtaa aatttttgcc
     9901 gagtcgcttg ctcccttcgc cggttcacac cagcagtctt cacaggcgtt acaaaattta
     9961 caacgtcaac tgggattaat agctggaggt gaggctggaa aacgggctgc aacggcagcg
    10021 ggtctccgtt gcagtgctga tactcttctt cgcagggtta tcaatacccc ggagacgaaa
    10081 cagtcaggcg cgcctcatgt cggtattgat gagtgggcgt ggcatcgggg ccactgctgc
    10141 ggtatgttaa tagtcaatct tgatactcac cgtcccctcg tcctgcttcc cggccgtgat
    10201 cagcgtacgc tggcgacctg gttcagaaaa tatccggaaa tacaggttgt ctcgcgtgat
    10261 cgcagtggag tctatgcgac agcagcacgt gaaggtgcac ctcaggccag acaggtggcc
    10321 gatcgatggc acctgctaaa aaatattggt gatgagcctg aacgaatgat gtacagacat
    10381 atgcctctga tacgtcttgt tgtcagagag ttatcactga agaaatcacc tgagccagaa
    10441 atatctgtgc ctgtagcatc gctccgtcgt ctggaacgcc ttaaacagca catccgcaaa
    10501 aaacggcatc agcgttggac agaggttatg gccctgcata acaagggatg tagtttcagg
    10561 gaaatatccc gtattacagg cctgtcgcga gtgacagtca gtcgctgggt gggttcagga
    10621 acattccctg aaatgtcaac taggcctcca aagcgagggc ttctggaccc atggagggag
    10681 tggttaaaag agcaacgaga atgtggtaat tataactccg gccggatatg gcgggaaatg
    10741 gtggccaggg gggttacagg cagtgaaacc atcgtcaggg atgctgttgc caaatggcgt
    10801 aaaggctgga tcccaccggt tactactgcc gcaagacttc cttcagtgtc ccgggtaagc
    10861 cgctggttga tgccctggag aataatcagg ggtgaagaaa attatgcttt ccgatttatt
    10921 agtctgatgt gtgaaaaaga accggagttg aaaatagcgc agcaactggt actcgagttc
    10981 taccgtattc tgaaaaccta aaataaatca cagctaagca gctggttcac tcgggtccac
    11041 gaaagcggct cagcagaact tcggtgcgta gctgcgggga tggaagctga tgctgcggct
    11101 atatgtgagg caatcagcag tcgctggagt actggtgttg tcgaaggtca tgtaaatcgc
    11161 ctgaaggtgc tgatacgcca gatgtatggt cgagccggac ttgaactact taggcggagg
    11221 gtaatgagtc cactggcatg aaaatcttca cccaggttgc ggaagagcca cttttgcact
    11281 gttcgtgaca gtatggcctt tatgtactca ctttgttacc ggtagctgga tcatcactac
    11341 cgtgctgcaa atggtggtga acagcatcaa ttgtggaatg aatgttgccg aagcgaccaa
    11401 tgcgcctcgt ttccactatc agtggttgcc ggacgagctg cgtgttgaaa agggtttagt
    11461 ccggatacgc tcaagctgct ggatgcaaaa ggtcagaaag tggcgctgaa tgaggcgatg
    11521 ggcagcacgc aaagcattat ggttgggtcg gatggtgagt tgtacggcgc atccgactcg
    11581 cgcttggtgg atgatttaac ggcggggtat taattgacat cccccggcgc aatgtgccgg
    11641 gggataatgg ttttatgcgg tcaataatcg tccgggatta tcgatacaga gttttttgag
    11701 gatcgtttta tcgacgccgc gctccgccag ataggccaga aaaacatctg gaacaaatcc
    11761 ccagccatta ccgccatttt tagcccacat ttgtttaagg aagacatcgt ggctgagaac
    11821 aagctgatcg gcgtatccca actcaatgag atgagcgacg gcatcggcag tctcctgcac
    11881 ccctggcgct attccctctt tcgggaaggt aatgtctagg ccaatcatgt cgaattccag
    11941 ccagacacct ttatcaagca ttttccgctg atacgccacg tcttttcctg acggatccga
    12001 gtgtgcgaga gagactttat ttggcgagac gcccatttca cctaacacaa tgtcgagtac
    12061 ttcatcaccg cgacgaagcc agcccggcat gtgaatattc atcgccacat gaggattgtt
    12121 aatctgtgcc agcgaggcag cccgcaagct gttatgctcg gcttcggtaa atgtcggtga
    12181 aacacctatt tcaccgatca ttccggcacg aatatccgta tcgccaatcc cttgattcaa
    12241 ttctttatcg atcgttgtcg ccagttcatc aaccgtttta tgaattctct ggctttcaaa
    12301 tttttccagg tagggaccgg aggaagcaac aatattcaga cctgttttta atgcgacttc
    12361 tcgtaatgcc tgcgcatctc gtccgataga ctcagaccct gtggcatcaa taatggtgcg
    12421 accaccgagc gagataaagt tattaatttc gaagatcaca tcttcaatag gtttgcggtc
    12481 catattatca gcgcagcaat aaggatcgtg ttttaatgcc cactggattt ctgccgtgac
    12541 ttttttatca acaagccgtt gtgaaaaagg atagcaagga gcatccacaa cagaggaaag
    12601 atcgttgaac agatgttcat gaggaagtgt tagccccata tcttcacggg cgacggggcc
    12661 cgtcacagtt tgtagataat ctttcatgat tatacctcca ttttcttcat tgttttcgag
    12721 gttctgtatt ccggaatagt ggtaacaatt gcaccgacca gtgcaaacag tgttccgatg
    12781 atcgtgacca gataaacgct attgcccagc gatggaatta atacgtcaat caatactgat
    12841 cccagtaact ggcccgccgt tgaagcaacc cccagcatca gtaaccccag acctctgacc
    12901 aaaatggcca ttaaaccgat agacattagt ccaagcgggc cgccaagata catccaccag
    12961 acatctggta attgaacagt gacgtgccct aatgcgacac gaatagctag cgctgtgcct
    13021 aaaacacaaa atccaacgat gaaattctag gtgattgaaa ccagcatgga tcccgtagct
    13081 tcagctactt ttgcatttcc agcgggctgc catcctgcaa gcaaaccagc taagaacgga
    13141 agaatggcaa gtaagatgaa agaggtagaa tgccactgag gtgacacaac aaaaatcgta
    13201 gcaataaccg caaataatgc accgatgatg cgataagggg taaagtattt tttctcgtcg
    13261 acaccgatac caaagcgatc gcaaagtaaa ccagacagca atagcgcgga gatcagtgcg
    13321 gtttgaaacg ttgcgacccc gagtgcgctg gctgatgcgc cttcagaaaa taccaccatc
    13381 gctccacaga gaccggcaaa ccagttccag agaggaactt ttcggttttt aatcagagaa
    13441 ggaattgccg caaattgttg ccgggtttct tttcgcgcca taataataaa aaacatcacg
    13501 actaaaccac tggcaaagga gataactgcg catgcattac catcctgtaa ccagtgacct
    13561 aattgaccat tcaccgctga ttgcataggg gagagcatac ccgcaagaat cgtggcaagc
    13621 atccataagg gagttgagta cttattcttt ttcatacatc aatccttcta ttatagtaaa
    13681 tggagtaact ggagttaccc cattattatt attatcttgc aatatgagtc agtatattta
    13741 cccacaacgc ggtatagaac ggccaggaca taaactgctg tgtaccccag tgcggtgaac
    13801 agtcactcat aaagcaggct attttcccgt tgtgatagtt accaaagacc agtaatggag
    13861 cgttgtttat tgttaatacc acctcagcat tttctttagc gatcgcccgg ttataaccta
    13921 ggaaaaaagg atattcagag aaccctttaa taactggatg ttcaggttgt gatggctgtg
    13981 caataacacc ctctggtttt tcgacacgat cgtcaccatc taacatcgta accgggagca
    14041 catcagccag aactgtattt ttataattgg cttttgcttc aattcccatg aatgagagat
    14101 agccgccaat catcaataac ccaccaccgt tattcacata ttctttaatc agttcgagag
    14161 cgtttggttt tatgcgaagc tgataaaaag tatcattttg tagcaggaaa gtattactac
    14221 caatatcact aataacgatg gcatcatatt gttctaactg tgccacatct tcagggaagg
    14281 caatttgtac cgtatgtgct ggcatatagg tgacatctac ctgactattt ttcagacact
    14341 ggagtaacca ggtcgcacct tcttcatatt tacttgaggt aaaactgtca tatccttttg
    14401 aatgaatcat atgaatatgc caggattccc caataaagag cacttttagt gattttttgg
    14461 tgttattcat tctctgtccc ttcttaatgc gtaaaaatag tttgctgata atgtatactc
    14521 tgaattcgat gtatcacaga ttcatgttca ggcatgtcgg aagcattgct tgtctccacc
    14581 gctagggatg caaaggcgct ggcataacac agtgctgatt ccagtgattt tcctttcgca
    14641 agtcctgatg ccagagagcc gttaaacgca tccccggctc ctgccgtatt tttaacgacc
    14701 gctggaaacg ctggcgaatg aatgaatttt ttgccatcaa atgcgagcga gcccttgcta
    14761 ccgagcgtga taaccgtgtt ttttacccct ttattgtgga tagcttctgc agctcgttta
    14821 gctgattcaa gatcgtgtac gtctatgccg gacagtaaac cagcttctgt ttcattgggg
    14881 gtgatataat caacatcctg aataagttcg ttgacgatat cattataggg tgccgggtta
    14941 ataatgaccg ggatactgtt tttttgcgcc aatgtgatcg cttgttgcag agcggtataa
    15001 tttgtttcga gctgcagaag tatgacatca gaattaataa tcgcttcttt ctgaatagtt
    15061 atttcatcgg acgacattgt catgttggca ccgggataga tcgcaataac attatcgcca
    15121 gtaccttcat taacaaggat cgtggcagtt ccggtttgcg tttctttcgt ttgataaatt
    15181 atacttttat aaatcttaga cgaattcata aagttgatcg cataatcact aaagtgatct
    15241 gtacctactt ttgttatgaa ataaacatca gtatcggcaa agcttgctgc cagcgcctgg
    15301 ttacagcctt ttccccccgg tgaaaatata aaattacttg ccagaagcga ttctccgata
    15361 gtgggaagcc tcggcaaata actgatgata tcaacattaa acgaaccaat aacgcatact
    15421 tgacttttca tagttgagtg gccttttttt attccctggg ctgtatcata attttcaagc
    15481 gcataagcga tttcattttt cgcaatagta tcaagcgagt ctaggacgat aaaagcaccg
    15541 ccatgacatc gagtcagata gccaagcctg gcaagttcat taagatctga acgaatagtt
    15601 tctttcgtta cattgaggct attggacaga tcggatactt ttacagaacc gaatttactg
    15661 agtcgtttca atatttcatg atggcgttca aatttcatgc ttcacctcac ttcagttacg
    15721 ttaatatcta tcgatgtata ttgatgtgat aatcaccata aagtggcggg gaaaagcaaa
    15781 aaaaagcaaa aatagcctga gaaaaggtaa gcgttaatgg agaatcatat tgatgcttat
    15841 ttattggtga gaactacgtt ccatggcagg agttcatcaa tacggttgga aggttattcc
    15901 ggcagtacgc tcaggatatg gcgcagatac gcttccggat caatgtcgtt cagaaggcaa
    15961 gtgccgataa gcccatacaa cagtgcactg cgctcgccgc cgaagaacat aaagttttta
    16021 tttccgagac agatggcaca aagggctttt tccgcggcat tatgtctgcc tccgcctgac
    16081 cgtcaacact gtaataacag agtgcgcccc actgattcag tacacagctg aaccattctc
    16141 ccagtctgga tttttggata gcgtggcatt tttctccacc atgcatgcag tgagtccagt
    16201 aacagtttgc tcttcttctg cctgaccaca agatgtgctg gactggtaaa cgatggggat
    16261 acctgcttac gcgagagcaa taagtggctg gccacaattc tcggtacaaa actctgctga
    16321 atagcagcga ttttcccagc gtggtgcgtc gtttgcattt tactggttct aagttaacaa
    16381 ttatctgttc actaatgact actctaaaaa cttgtatcag gactatcact tatttaagtg
    16441 atattggttg tctggagatt cagggggcca gtctataaac tttttacaaa aggatgaatg
    16501 cagaaggtta ctctaatggc cagagtgatg acaatgatgg tcagacgttg acctgcagaa
    16561 actgcatttt aattggtcta taaagtctgc aagaggaatc agggtcagat cagtgagtct
    16621 tatcgcggcc cgaagctatg actgaaagaa ggctggatac agaattgcaa caatttggca
    16681 atgtcagaac acagaagctt tgcaagcagg tgaaaggtta tgtc
//
LOCUS       DAZFAH010000097        16714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFAH010000097 DAZFAH010000000
VERSION     DAZFAH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.097"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..655)
                     /gene="yghD"
                     /locus_tag="ACHJWB_002907"
     CDS             complement(119..655)
                     /gene="yghD"
                     /locus_tag="ACHJWB_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO4314502.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGLSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETILQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(657..1835)
                     /gene="gspL"
                     /locus_tag="ACHJWB_002908"
     CDS             complement(657..1835)
                     /gene="gspL"
                     /locus_tag="ACHJWB_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO4314503.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1832..2809)
                     /gene="gspK"
                     /locus_tag="ACHJWB_002909"
     CDS             complement(1832..2809)
                     /gene="gspK"
                     /locus_tag="ACHJWB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO4314504.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2806..3411)
                     /gene="gspJ"
                     /locus_tag="ACHJWB_002910"
     CDS             complement(2806..3411)
                     /gene="gspJ"
                     /locus_tag="ACHJWB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO4314505.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQCGEIERIWLLRGPQLS"
     gene            complement(3408..3779)
                     /gene="gspI"
                     /locus_tag="ACHJWB_002911"
     CDS             complement(3408..3779)
                     /gene="gspI"
                     /locus_tag="ACHJWB_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO4314506.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHKGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3776..4339)
                     /gene="gspH"
                     /locus_tag="ACHJWB_002912"
     CDS             complement(3776..4339)
                     /gene="gspH"
                     /locus_tag="ACHJWB_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO4314507.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4343..4798)
                     /gene="gspG"
                     /locus_tag="ACHJWB_002913"
     CDS             complement(4343..4798)
                     /gene="gspG"
                     /locus_tag="ACHJWB_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO4314508.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4815..6038)
                     /gene="gspF"
                     /locus_tag="ACHJWB_002914"
     CDS             complement(4815..6038)
                     /gene="gspF"
                     /locus_tag="ACHJWB_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO4314509.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLRNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6038..7531)
                     /gene="gspE"
                     /locus_tag="ACHJWB_002915"
     CDS             complement(6038..7531)
                     /gene="gspE"
                     /locus_tag="ACHJWB_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO4314510.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7531..9591)
                     /gene="gspD"
                     /locus_tag="ACHJWB_002916"
     CDS             complement(7531..9591)
                     /gene="gspD"
                     /locus_tag="ACHJWB_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO4314511.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9621..10580)
                     /gene="gspC"
                     /locus_tag="ACHJWB_002917"
     CDS             complement(9621..10580)
                     /gene="gspC"
                     /locus_tag="ACHJWB_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO4314512.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10598..11008)
                     /gene="gspS2"
                     /locus_tag="ACHJWB_002918"
     CDS             complement(10598..11008)
                     /gene="gspS2"
                     /locus_tag="ACHJWB_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO4314513.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11074..11883)
                     /gene="pppA"
                     /locus_tag="ACHJWB_002919"
     CDS             complement(11074..11883)
                     /gene="pppA"
                     /locus_tag="ACHJWB_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO4314514.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12031..16596)
                     /gene="sslE"
                     /locus_tag="ACHJWB_002920"
     CDS             complement(12031..16596)
                     /gene="sslE"
                     /locus_tag="ACHJWB_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO4314515.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIIL
                     SEGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLS
                     NGATLGEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKS
                     SMTVGTNLDTVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNG
                     FEYVTQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTID
                     DTTKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQ
                     RILDAFPGLEVCKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQ
                     AADVGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKND
                     ELGFKTFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNY
                     MEKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQST
                     GLWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTV
                     KFKVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAF
                     VYTTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKH
                     RFTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPG
                     ATEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQL
                     KEWAEKNFDIKKWYPEGELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYC
                     AESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNT
                     LASLKLPKPEQGPENINKVTEHKMSVE"
BASE COUNT         3679 a         4747 c         4224 g         4064 t
ORIGIN      
        1 gtttttgctt ttggatctga caataacctt cacgaaaaaa gtttgcttat aaagtctggg
       61 ggaattactc tcgccacgtt aacgagagta attttattga tactaatctc ctgatacttt
      121 acccccgtcc aaactccagc cgctgcacat tcaccatccc agtcttctca gcagcactgg
      181 catcaatttg tgtcacccgc agcgcatact tttcatccag cgcgtttaac cagttcagca
      241 ggtcattaaa caccaccgga tctatccaga cctgaatatt ctccccacgc tcggctatcc
      301 gcttgatgac taccgagtgc gcggaagcac tgtcactgat aacccgcgat acctgcgcag
      361 gcgttgttgt ggcggatttt cgcgccgcaa taatatccgg cgcggcgctc ctcagtctgg
      421 tgttcatcgc caccagttgc tgcaatattg tctcctgttg tactatccgt tcgctcaacg
      481 gctgccagat gaggacgtaa tatccgacgc taaacaggaa caccaccgct gccagtaaca
      541 tgcccttttc acgcggagaa agccccgcca ggtgttgtgc cagccagtgt tcgccacggc
      601 ttaactggcg ttcacgccat tgctgaaaat agtgaataaa tttatcgcgt aacatgttat
      661 ttcctccgca acgttacgcc gccggaaacc gcatcaccct ctttctgtaa cgcgtcctgt
      721 tgcacaacat aatcggttgc cagtgcacta cgcagtttgt cgaaactggc aaagtttgct
      781 gcccgcaact ggaggtgaag cgtctggcgt ttttgatcaa aggtgaaacc acgcatttcg
      841 atgtcggaaa gtgacgctga tttcagggtg ctggctatcg ctgacaactc ggcgagcagc
      901 cgggtatcgt cggcctgtgg gcgatatttt ttcagcgcca tcgtcacctg agagcgtaaa
      961 ttcacaatcc gcttctgctc cgggaacaac gttaagaact gttcctccgc ctgggtgcgg
     1021 ctttgcgcca cctgttcgct gacgctccat aacgtcacgc cccgctccac tgccagcgca
     1081 accagaatca gcaatatcgg cagaatcatc acccgccagc gcgcccactg ttttcggtag
     1141 ctgacgcgag gctgccacgg ccctgtcagc aggttccctt ccggtttgcc ataagtggta
     1201 acggcgggca gagctgtaac ggtcaggcgt tcagcgtctg cgccagccca tgctgatagc
     1261 ttttccggtg caatgccgac tacggttagc gaaagcggta aatcctgctc attgagctgg
     1321 gcgcggaaca tgaccggagc cagcgccagc ccggcgctcc atccccggca ttcatcgatg
     1381 cggcagataa cccgttgcgc atcgccagcc ataaacccac aaggaatgga catccagtcc
     1441 ggcgcgacgg tagcgcgggt gatgccgttt tcctgcaacc actgcgcaat gttgcgcata
     1501 tgctgttggt gaatcactgc tacggttgcc agttgctggt cgattttcaa cggggcgaaa
     1561 tgcagttcat cgatatcctg gttcagctct tcttccagca aggcgggcag aatagtcggt
     1621 atctgcttgc gggcgacatc aggcagttca acctgccaga cgctgatcca ttcgccggga
     1681 atgtagagtc gaatcgcatc ggtttgcagc cattgctgaa gctgttcgtc agcaacgtca
     1741 ggccagatac cgtgttccac gtcgggtgta cggcgctgcc agcggacggt atcggaggcg
     1801 caaagcggga aaaaaatctc aaggatggaa ctcactcact ttctcctgtc tgatgccaga
     1861 gaaccgaaaa gtgttgtggg cccatgcgga caattaacga attcatcgtc agttcaatct
     1921 cattcacggt gatatctgaa cgcagccaga agtaattgct gtccacgctc aggacggttt
     1981 ttagctgttt tttagtacgc tcatcgacgt cagcaagtag cggctgtgca agaaactgat
     2041 cgacatcttc ccagcccttc gccggacgtt gttgtaataa tgcccgcgcc tgaacagggc
     2101 ttaaccacgg gtcaaacagc gcctcaagaa gcacactttg cgtgacgtct aaggtattga
     2161 tgttgatttg ctggcgggtc atcggcagcg cacagaccag cggtttcagt ttttgataaa
     2221 gcccgtcgtc cattccctgc accacgcgca tctcgctgat atcggccagc ggttgattgg
     2281 cggcgtagaa cggcaccgag cgggcgagat actcgctgtc ttcacggccc agacgcgtct
     2341 gcacgctgcg gtcctcgtca ataaactccc acaggctttc ggctatcagt tcggcccgat
     2401 aagcaggcac atcgaggcgc gagatcaggg caatcagttg ttgtaccgcg agcggacgcg
     2461 acgccgttgt gggctgagcg agggcattca ggttaaagca ggcctgtgcg tcacgcagag
     2521 tgacggcgat ttgcccctgc ggcagtggga aaaaacgagg gccagaagcc cacggttgtg
     2581 ccagatgcac gcgcttttca tttttcaggc tcagactgag tgcactcagc gccaggcttt
     2641 ctgcactggc gctgtaccac agcgcctgct ggtactcctg ctggctgcgc gttcgcccaa
     2701 gttgttgctg catccgcccg gaaagcgtga tggttaccag catcataacc gccagcaata
     2761 ccagcaccac aaccagtgcc attccgcgtt ttggtggtga ggtgatcatg ataattgcgg
     2821 cccgcgtaac aaccagatgc gttcaatctc gccgcattgc ggcgaatgca gagtcatgcg
     2881 cactgccaca gggatcgcct gtactgacga ccagctctcc tgccagcgcg taccatcata
     2941 gaactgcaaa cggagcgaat ccgccggaat caacttttgc attgtcggct tcacgctgcc
     3001 tgcggcatcg gtcagcggcc aggctaaccg ttcgagataa ccgtcatgaa tgcggtaacc
     3061 gacggtgagc agattgctgc gtggcagacg catcaacggg ttaaccacgc cgccgcgcac
     3121 aaagcgcatg ccttcactct cagacgccag cacgccagcg cccgccagta acgctggttc
     3181 acgctggccc tgttcgcctc ttaccggacg cggcatcatt tgtgtcagat cgtgggtcag
     3241 aaaactcatc gtttgctgca tgaggtttag tttttgatca tgtccggcga cggcgttatt
     3301 cacgcgtgtg acaccgtttg tcacctgctg cgccatcagt gccagcgagg caaaaatggc
     3361 aattgccacc aacatttcca gtaacgtgaa accagcgcga gtccttctca ctgttggcct
     3421 cccacggcgc taaaccaggc gcgtcgtgac tgaatcaccg acgaaaagtc ttcgtgacgg
     3481 ctgacttcaa tatccaccgc ctggagcagc gcgttgcctg tattcaatgg gattgttcgc
     3541 cagtaccagc ggcttcctgc catatcgctc tcgcccttat gcctgaccgc ccgctgctca
     3601 cgggtcatca ggtagagcag cgcagtctgg ttttcagcta cccagcccgc taccgttttt
     3661 tcctcaagaa cgtgctgatt actcagcgca ccgctggcaa tctgtaacac cgccgtggca
     3721 gccagcgcaa aaatcgccag cgcgagcatc acttccagca aggtaaatcc gcgcttcatg
     3781 gcatcctctc atcacattga ttgagggata acgcgccatc gtgtgccagc tttaccgccc
     3841 aacatgcgtt ttgcgccgcc gagtagaagc gcagcgtaaa cggcgtggcg ggttcaaaag
     3901 gcgaaaaacg gatctgcgga gtcttctttt tcgcctcttt ttgcagctcc agttcaatat
     3961 cgtgcagcgt cagtcgacga cgttgcagct ccagcgcata ttccttctgc cagatatcac
     4021 ttccgggttg taacagcatc tgcacctgtt tcggcaccgt aacctgtgcc gataaccggg
     4081 tcgaggaaac gggcgaccac tgcccctgac gacgctgcat aaactggtag ccaggcgggt
     4141 cgattagcac accgagtgtt ttcccttcga tcactgccct gtccttaaac tgcgcaaagc
     4201 gagtcagaaa atcctgcgcc gcttttttcg cgggcgattc agaatcggtc gcaaaagtct
     4261 gtaccacgcc cgcactggca aggccgataa gaaaaatcac cagcatgatt tccagaagtg
     4321 tgaatccgcg ttcaggcact gcttactgaa actcctgcaa attccagtta ccgatatctg
     4381 cgccagcgcc ttccccgttt tcctgaccat ccgcccccag ggtataaaca tcaaacagcc
     4441 ctttttcacc cggactgaga tactgataat cattgcccca cggatccttt ggcagtcgtt
     4501 taatgtatcc accagtacgg tagttgcggg agtccgccat attggctggc tgctggatca
     4561 gcgcctcaag cccctgttct gtggtcggat aacgcccgtt atccagtcgg tacatatcca
     4621 gtgcgttttc cagcgccacg atatcgctga tggctttttg ccgatcggct ttctctttgt
     4681 tgcccaacag gttaggcacc accagacttg ccaggacgcc aagaataaca atcaccacca
     4741 tcacttccag cagggtaaaa cctgcccgtg gtttttgtgt gcgggataac gaattcatgt
     4801 gataactccg taaattacat tccaaccata ttattcagtt gcagcatcgg ctcgaggatg
     4861 gcgatgacaa taaacagcac cacgcccgcc atcatcacca ccagcgcagg ctcaaacagc
     4921 cccaacgcca gccccacctg ggtatcaaat tcccgttcct ggttaacagc ggcctgctca
     4981 agcatggttt ccagttcgcc gctctgttcg ccggaggcga tcatgtacag catcatcggg
     5041 gggaacaggc gcaactccgc cagcgcggcg cgcagactgc ttccttcgcg gacgcgatct
     5101 gccgccagca gtagttgttg ttcgacatag cgatttgccg acacagcggc agcggtctga
     5161 ataccttcca gtagcggaac actgctggcg gtgaggatgc ttaaggtgcg ggaaaaacgc
     5221 gccgtattca gcccgcgcgc aacacgccct gtcacgggca gacgcagcac ggttttatcc
     5281 cagcgcaggc gcatagtagg atttctaagt agccgttgcc ccagcaccag aagcgccagc
     5341 aatcccgcca gccagtaaac gccgctggcc tgtaacgcgt cgctcatggc aataagcgtg
     5401 cgggttgagg cgggtagcgc gtgtccgaga tgatcaaact gttcgatgat tttcggcacc
     5461 actgccgtca gcaaaatagt gactacgccc gttgccacca ccagcagaac cagcggatag
     5521 agcatggcct gcaatagacg tgatttcagg cgctgtcgct gttcggtgta atctgccagg
     5581 cgattaagca ccacgtcgag atgcccggat ttttctccgg cagcaaccat cgaacaaaac
     5641 agagaatcaa agacgcgggg atgttcgcgc aggctgtccg acagggtgta accttcctga
     5701 atccggctgc gcagcgccat tccgaggctt tttacatgca gtttttcact ttgctcactg
     5761 accgcctgta agcaggtttc cagcggcatt gctgcctgta ccagcgttgc cagttggcgc
     5821 gtgaacagcg caagatctgc cgccgccacg cgacgatgtg cgtgccgccg acgctgcaac
     5881 atcccccctg acgaagtatt catccgggct tcaatatgca cggggataag ctctttaccg
     5941 cgcaacaact ggcgggcatg acgcgcggaa tccgcctcaa tcataccttt ggttttgcga
     6001 ccattacgct ccagcgcctg atagtaaaac agtgccatta cgcctccatg gttacccgca
     6061 gaacttcatc gagagaggtt tctccggcga gcactttctc aatgccgttg ctgcggatac
     6121 ccgcagagtg ttgtcggaca taacgttcca gctccagctc cccggcctga cggtggatca
     6181 aatcacgcaa tgtggcatcc accacgatca gctcatggat ggcagtccgt ccgcgaaaac
     6241 ctttgtgatt acaggcggga cagccctgtg gatggtacag agtgacggta cgggcgtcgg
     6301 taattcccag caggcgtttt tcttcgtcag tggctggtgc gggctggcgg cagtcgggac
     6361 acagcgtgcg aaccagtcgc tgcgccatca cgcccgtcag actggaagag agcaggaaag
     6421 gctccacgcc catatcctgc aaacgtgtga tcgcccccac cgctgtgttg gtatgcagcg
     6481 tggaaaggac caggtgtccg gtaagtgaag cctggacggc gatttctgcg gtttcggtat
     6541 cgcgaatttc accgaccatc accacatccg ggtcctgacg caaaatcgcg cgcagcccac
     6601 gggcaaaggt catgccgacg cgggtgttaa cctgcgtctg accgatccct tcaatcatgt
     6661 attcgatggg atcttcaacc gtgagaatgt tgcgcgaatg gttgttcagc tcctgcaatc
     6721 cggcgtacag cgtggtgctt ttgccggaac ccgtcggccc cgtcaccaga aagatgccgt
     6781 gcggtttgtg taacagctgg cgcaactgcg cggtcagttg ctggctaagc cccagacgct
     6841 ccagcgtcag gcgggcctgg tttttgtcca gcagtcgcag caccacgcgc tcgccccagg
     6901 cagaaggcat ggtggagaca cgcacgtcaa tcgcccggcc gcccagcaac agcgcaatac
     6961 gtccatcctg cggcacgcgc ttttcggcaa tgtccagccg cgccatcacc ttgatacgcg
     7021 acaccagcag cgaggccagt ttgcgccccg gacgcagcat ttcatgtaat gtgccgtcaa
     7081 cacgaaaacg gatcaccaga ctcttttcaa acgtctcaat gtggatatcc gaagcgcctt
     7141 ctttgattgc ctctgccagc atggcgttga tcagtttgat gatcggcgca tcgtcgtcgc
     7201 tttccagcag atcttccgtt tccggcagtt cttccgcgag ggtaaaaaag tcctcggcag
     7261 aaccgagatc ttccatcagt tggcgagcct cggaagagtc ccgctgccag accgcattca
     7321 gccgctgttc aaattcggct tcgtcgattt gccgcagcgt aaagggcgcg ttcagccccc
     7381 gctgcagctc ctgcaaaaca gagagcgaca acggatgaac atggaggatc tccagcgacg
     7441 cttcgcacca tgccaccagg ctaaaccgac ggctgaaact gtagggcaga cgcacggtgt
     7501 tagcggtggt ttcctgtgct acaggcacca ttaacgcgtt ctcccggcat taaggaacgc
     7561 acgaacttcc ggcggtagag cctgattttg cgctggcagt accggctgcg cggtgtgcgg
     7621 catcaggctt aaaccttgct catcgcgata gatctgctcg gcgcgcatgt agttatattt
     7681 gcgctgcgac acgccgtctg ccgccatacc gtcacgcaga atggtcgggc ggataaacac
     7741 catcaggtta cgtttttctt ttttatccgc cgtcgattta aacaggttac caatcaatgg
     7801 gatatcgccc agcagcggca ctttcgccac gctttctccc gcctgatcgt ccatcagacc
     7861 accaagcaca atcagctcac catcgttagc cagcacggtg gttttcagtt tgcgctcacc
     7921 aaacaccacg tcgaggctgg tctgtccttc caccttcgag acttcctgct caatcaccat
     7981 ctgtaccgcg ttaccttcgt taatctgcgg cgtgactttc agcatgatgc cgactttttt
     8041 cctttctact gtattgaaag gattgctgtt attggagcca acggtagagc cggttaatac
     8101 cggaacgtcc tggcccacca tgaagaaggc ttcctggttg tccagcgtag tgatgctcgg
     8161 cgtggagaga acgttcgagc tggagtcgtt tttaaccgcc tgcaccagtg ccatccagtc
     8221 gcctttcacc acgccaaccg ccgtaccgct aaatccagaa agaagctggg cgagtgtgga
     8281 gagatcgccg ttggtatccg gattaatggt ggtagcgccg ttttcactga tcaccgtcga
     8341 gcctttctgc ggtttcgcct gagaaatggc tgcccccagc gtaccgatag ggatctgcgt
     8401 accattagca aactgcatta atccggcatc tttcgacgcc cactgcacgc cgaagttgat
     8461 attgctacct tcggcaactt ccacgatcaa cgcctcgacg tgcacctgag cacggcgaat
     8521 atccagttgt tcaatcacgc tttgcagcga ctgcatgatg tcctgcggtg cggttacaat
     8581 cagggcatta ctgtgtttgc tggcggcgat ggatacaatc tcacgcccgc taccgaccgt
     8641 gccttccgcc tcttctttag ccgccgtgag cgtgccgctg acctgcttca gcacatcgac
     8701 cagatcttcg gctttgctgt atttgagata gaaaacctgg ctgttgccgc tgcgttccat
     8761 ttctgagtcc agccgacgga tcaggcggcg cattttgtcc cgcgtggccg ggtcaccact
     8821 gacaatcaca ctgttggtgc gttcgtcggc gacaatttga gatttcagcg tcgctggctg
     8881 gttctcgccg ctgtttttcg tcaggctttc cagcacgcgg gcgatttccg aagccgaggc
     8941 gttatccagc gggatcacct cttcggtgcg attacccgcg tgatccacgc gctggatcac
     9001 ttccgtaagg cgctccacaa cggaggcgcg tccggtgagc ataatcacgt tggagggatc
     9061 gtaattaaca acgttgcctg agcctgcgct gtcgatcatc tggcgcagaa tcggtgccaa
     9121 ctcgcgtacc gacacattac gtaccggcac tactttggtg accatttcat cgcccgcgta
     9181 gttgtcgctg ccttcaccga ccagcggcag cggctcgact ttcgcagcgc tggatttcac
     9241 caccttcagc acgtcgtttt ccattggtac gacggcatac ccctgtgctt ccagcaggtt
     9301 aaggaatagc tggtaatact ggcgttcatt gagcggggtc atggtgcgaa tactcacttt
     9361 cccctgtacg cccggcccca taatgatggt tttattaagg ttagcgccga cggtttcgat
     9421 gaacgatttc aggtcggtat ctttaaaatt agcggtgaaa gtggcttctt ccgcccagac
     9481 cggtgaactg cataatgccg ctgccagcac cagcggcagt aaacgctttt ttgttttgag
     9541 gccagttgtc ttcttacgcc agaccgacaa cgtaatatca cgccaaaaca cgatgaatga
     9601 ttctcctgga ttaaatgcgg ttagcgcagc gcgatggaaa tgtcgtggcg cgcacccttg
     9661 cgtaaaaccg taagttgaat ggaatccatt gaaggtaact gccgcatcag agcaatcatt
     9721 gctcgtggat cagtgaaatc ctgctgattt agcgcaatgg cgatatcgcc ttccttgaaa
     9781 ccgctggcat cgaacagaga acgatctgcc cccggcttca ctgcataacc gacaattccc
     9841 tccttacgca caggcgtaag ctggatatag ttaaaaattt tctgcggatc tttcgccagt
     9901 gcctgacgca cggcagtcgg tatctcaact ggcgcactga cagcaggttc agcaacagct
     9961 tgctttgctt cgtcactgac agcttttttg ttggtcgcgg caacggtgga acgctcctct
    10021 tcagccaggc tcaggcgctc tatttttccc tgatagcgca gcatcacatg gtcgcgattg
    10081 atttcctcaa tcaccgcatt gtgcgagcca agcgtttcac cctgcaaata gacctgctgt
    10141 ttaccgcctt cttcaataac cgcgccgggt ctggcaccaa aggcaatccc gcgcagcacc
    10201 acattaagac gcgtttctgc cacaggcaca ggttcgggtt gttttaccgg cgtggcgacg
    10261 ggctgatatt tgccaaacca gttttgctgg ctgattaatt gcacgtcatt tttatcgaac
    10321 gtttttgcat ccgcacgggg cggtttattc gccgatgggg aaacagccgt atattccgca
    10381 gaaaaggaga aatagcgcca gagtgaatgc gccacttttg cagaaataat aagcagcatc
    10441 agccagaaca tcccgcgtac aatttttcgc agatgctctt tgtctgtatt aaaagattgt
    10501 ctctggttaa gagtgtgacg aatttttgtc agccattgaa tgagataaat tcgtgcgtca
    10561 cgaaaaacaa cccgcgccaa tttatatcta cccgacgtta tgctttgact attccacagg
    10621 tggtacgatc cagtttccgc tgatactggt tacctgtacg tgtatcatta atcgttatgc
    10681 tgtaattaat tccctcggta atcattaatt tcaccgttgg gtcagcgcac atttgtcgtt
    10741 gatagctggt taaaaaggcg tcaggcgtct gcgtggtctg agtccccgct tcgctgataa
    10801 tggtcatttt taccgtcgtg ccgctacttt gtgccagcac taaggtatat cccgcagatt
    10861 taatcggcag gttttggctg atattttgcg cctgtttttt cgccagtaaa ctggcatttt
    10921 cattatggct ggcacagcca cttaataatc ctgtaacgct caacaatagc gatattaata
    10981 ccctccctgg catttgtttt atcgacatag taaataatct ctaaggttat taataagagt
    11041 taaaatgtca ctttgataat gacgtggtta tcattaaaac aatgcctgta gataaagtgt
    11101 tgctataccg cccagactta aacacggtcc aaaaggcagt gtggttgatc ctctttttgt
    11161 aataatggca tatatcaggc cgcagcatga tgcgattaaa gcaacattgg gcagtgataa
    11221 cggccccacc cagccaccta acgcagcgaa caataataca tcgcccatgc ctaatgcttc
    11281 tttacgcaga actattccgg ctatccagcg cagggagtaa aaagtgataa accccaccag
    11341 gacgccggta actgcgtctt gtagcgttaa cggactctgt tgcgcccatg ccgcaatcag
    11401 tcctgtccac aatacgccct gagtaaaaac atcgggcagc cattggtgat cgaggtcgat
    11461 gatgctcgcg gcaatcagcc aggcggataa tatcatcacc gccagcgccc atccactttc
    11521 cggccagacc agactcgcca gcaaaaaagc gagtgccgtc aataactcca ccagcggata
    11581 acgcttgctg attttcgcct gacagtcgcg gcagcgccct ttgagcatca accaggagag
    11641 cagcggaata ttgtcacgaa cgcggatggt ctgctgacaa tgtgggcagt gcgaacgcgg
    11701 tagcgcaagg cttatttttg actgcgcact cgacatttca ccgtgaaact ccgccatttg
    11761 ttggcgcagc atgatggggt aacgccaaat caccacattc aaaaaactgc cgatgatcaa
    11821 tcctccgacg gttgccagta tgggcatcgc cgcggggtat tgctgaaaaa catcaaaaag
    11881 catggttaaa ggttgtttgt tgtaacttgc tggatgcggg cctgcaagtg tatgccatac
    11941 ggctttcctc caggcccgat aagctggcgc aacccggtgc gccttatttc atgccggatg
    12001 cggcgtgaac gccttatccg gcatacagga ttactcgaca gacatcttat gctcggtaac
    12061 cttgttaatg ttttccggtc cctgttccgg tttcggcagc ttgagtgacg ccagcgtgtt
    12121 gtacgccgac tggctcacac cgccctcgaa gctcatctcg ctcgcccccg gcagctggta
    12181 agcattcgca cccggattcc atttcttaaa gaactccgaa agatccgtct gggcgaccca
    12241 ggaggcacac agcatcagcg tgtccgcagc gttaccgttg gattcagcac agtaattctt
    12301 gccaccaaag gtcttgtcac caacgtcatc gccgcgcgct ttacggtgca tcaactggaa
    12361 caggttccag cctttcatcc cttcacgatc gctgaagaac ttcggcaatt caccttctgg
    12421 ataccatttc ttgatatcaa agtttttctc tgcccattcc ttcagctgcg cgtacatcag
    12481 cagacggtca cccgcaccgc cgcgcgccca tgcctgaccg ttgctctcct ccagatattc
    12541 cggtgcgacg gtaatatcgt cagcgacacg gttcatcttg ccgagatagc gatcctgcat
    12601 gtacagcgcc agcacgttgt tcgcgacttc agttgcaccc ggtacagtca gcggtgtttc
    12661 tgcggcgttg tgccctactt cgtgccagat caaccagtcg ttcagcggcg tcgtcggcag
    12721 cgtggtgctg ttcgtcgaga agctgctgtt cattaccgga taacccgagt gcgcatcacc
    12781 aatggagatc tgcacatcgt ttgtgaaacg atgtttatgt cccgtcaatg ctttataggt
    12841 aaacatccgg tgcttaccgc tcgtttcatc acgaccgtag aagtcattca tcgagctggc
    12901 aaatgtgtcc agatcgttag cgaattgctc cagtccgcca gtgtaattgc tggcattcag
    12961 gttcttcttc ggtgtggtat agacgaaagc gtctgattcc agctcaccca gcggagccgg
    13021 tgagttcaga tcgtttttcc atgcgccgtc tttatagaac ggtgctttta ccacgccagt
    13081 aaaggtgaag ctggcagatt cattggtaga gctattgccc ttgatataaa tcaggccacc
    13141 gtaaggcacc ttgaacttca ccgtaccgct agcgtccaga gagtacgttt tagtcactct
    13201 tggcggacgg ttcagcgcaa cttcatgctt ctcacgtccg gtcaggtcgt cagccagcgc
    13261 cacggtgacg gtcacaggaa cgttcgcatt ggacttaatg gtgacctctt tctgagccgg
    13321 tgcccacagg ccagttgact gcatgttacc tgcaaaccat ttggtcggat tcgagtacag
    13381 gctgatggtt tcagtaacgt tctgtccctc ttccgatact gctcccggat acttctcgac
    13441 atcaactttg atgttcagat cccaccagga acggcccagc atcaggcgtg tcagcggttt
    13501 ttccatatag ttgagcgggt agctcgggtt catcatgccc gctttgctgc taccgtcacc
    13561 gtagatcatg ttgttatcga ccagcgattt tttcagatct gcagaacact tggtgccgcc
    13621 tgcataggca tcattggcgt agcagttcag gaactcggtg aacgttttaa agcccagctc
    13681 gtcatttttg ccttcttcat aacgatagct cgtatcgttc cacagccaga ccgacatgtt
    13741 ctggtacaga cgttccagat cgacgctgct cagacgctca cctttgcgac cattggtccg
    13801 gaagtagagc tcatgctgat acagacgctg aatgttggtg cctacatccg cagcctgcag
    13861 catcgctttc gcagtgtcag ctccaagatc cagctgcgta tactgcggaa catacatgcc
    13921 accggttacc ggcacacccg tgcctgggcg gtattccaga cagttgacct catagtgata
    13981 gtcagaatcc ttacacactt ccagccctgg gaacgcgtcc agaattctct gttttgccgc
    14041 agccagtgac tcaggcgttt tgtggtcggc ttcatcaata aaggcgaatt gggttacctg
    14101 tttaccctca acttcttcct gccagctggc aacttccagc ttcggtttgt catcaggttt
    14161 gttttcttgc tgatatttcc agataacttc tttcgtggtg tcatcaatgg tatacggtgg
    14221 tttaccatcg acagccggat aacgctcata gacccaaatt ggcgttgaac gtcgttgacg
    14281 aacacggtca gggtagcctt gcggatcgtt atttactacc gacttgttaa gcgccatcga
    14341 caaacctgcg gcatcaagca gacgtacaaa gccagatgcg ctctcttcct taagattgct
    14401 catcacgttt tccatgatca gcaccgatcc acctttgttc atataggcga tcaaatcggt
    14461 cacatcctgc tgggtcagct tcggtttgct ggtatctgcg cgcagaggaa tggagtaagg
    14521 atcgctaccc cattgtgtga cgtattcaaa gccattgagg atcaacagag gaacttcatc
    14581 tggattcaga ttgccatagc tggtcattgg tttgaccgtg atgccagtga aatccttgtg
    14641 gaacgcaaac ggtgcgctat ttcccagcac ctgaccatgt tttttgaaat ataccgtgtc
    14701 caggttagta cccacggtca tactggattt tgcatccggc aaccagcgat catttgacag
    14761 atagcgcagc acgttctcca tgaagttctt catgtcatcc gggtcgctgt tgagcgtaca
    14821 ctgtccgtct ttattaacgc ccccgttcca gctgtaaccg ttcgggcaac gcaggatgct
    14881 gttgtagtgt gggttaccga taaccatcag cttgccatcg cccacttgcc ccagcgagat
    14941 aaacggcagg ttgaaggtgg cggtttcgcg tgtcacgttc tctggtcgca caatggaagg
    15001 cgcttcagta atgtacgcca gctcattttt atcccaggcg cgtttttcgc caaacgccag
    15061 ccagtagttt ttatcattac gcgccatcag aatcgggaag gccgcgttgg agatattcac
    15121 caccgcctga ccgcgcgcat tacccgtgct gccatagaag ttggtagagt catggaatac
    15181 atggaactta ctgacagatt tgtagttcgt atccacgccc cacagcttgt tgataacgcc
    15241 ctgaatctgg ccgtcattaa cattgcgcgt cgtcagcgag aaccagcgag cctcgttaca
    15301 accatcggtt ttcgcacaaa tcgcggtatc gatctctttg gcctgacccg tcttaaactg
    15361 ctcgataaat tcgttaggca gattaacgac ttgctcacct tcccccagcg tcgcaccatt
    15421 ggataacgag agattgataa tctcgttaat cacgttggga tattcggcaa agaccttgcg
    15481 tacatcatcc ggaacaacac gggtattatt ttgcccggtc gtcgaatagc gatgaataag
    15541 ctgatcgata ttcgccccgc gaacttcatc acccagttca gtcagcgcaa tggtcgactt
    15601 attgccgcgc actgaaccca gttcaaaggt atcgatacca aaggagatgg tttcgcccca
    15661 gctaaaagaa aattcaccat tttcccctgt cacgccacgg cctgaattgg tgtagtagtt
    15721 gacgccagca acaccatatc cctggctatc caccaggcga ccttctgaga gaatgatttc
    15781 cgaaggttga tactgataaa actgttccgc gttagccgac acgaaggagg cgttcagatc
    15841 cggttttgtt cccggagtcg tgacggggac taccggtgaa gtatgagtgg atggcgcttt
    15901 atcagtcgcg gcattgtttt ccacctcttc attgaccagc tttttgaact cttccggtgc
    15961 cagatcgatt tgcttataca gcgagtcgaa acgtttactc tcgatcaccg aggagaactc
    16021 caggcacacc tgttctgtat tcgccgggca actgttcatg gaggtgacca gcgagagcgc
    16081 attgcttttc ttgttgtcgg aacccgccag ttcttgcgcg tcctcaagac taaacgacac
    16141 tttttcaacc gcacgcaggc tacgcgcagc ttctgactgg gtgtcgaagg tagcaattgt
    16201 cgtgttgttc cctgccacac aggtgacttt gtcgcctggt gtaaaggtaa agccatcgct
    16261 ggattcatca ttacaggtga tatcaccagt tacccgcagg cttccgccca gggtcagata
    16321 acccgttttc gtaggaacag gttctggctc cggttctggt gttggctcag gatcaggtgt
    16381 cggttccggc gtaggttctg ggtccggcgt tggctcaggc gtcggctccg ggtttggtgt
    16441 tggatcaggt ttcacttccg gcaaagaccc tgttccagaa tctacaggcg gcgtatcgga
    16501 ggaagatccg gagccaccgc catcacaacc ggctaacagg gttgcactca aaatagccgc
    16561 taaaagcgat ttcttatatt taaatttctt attcattaat aacgcaagtg acaaaacaat
    16621 gttaaaaaat tcgtaactgg gagaaatagt tttatgcttt atcttcttta ataatttcct
    16681 ccattcttaa gaaaacgaca tcattgatag aaaa
//
LOCUS       DAZFAH010000098        16686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFAH010000098 DAZFAH010000000
VERSION     DAZFAH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.098"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            251..304
                     /gene="ychE"
                     /locus_tag="ACHJWB_002921"
     CDS             251..304
                     /gene="ychE"
                     /locus_tag="ACHJWB_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4314516.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            335..982
                     /gene="ychE"
                     /locus_tag="ACHJWB_002922"
     CDS             335..982
                     /gene="ychE"
                     /locus_tag="ACHJWB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4314517.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1140..1436)
                     /locus_tag="ACHJWB_002923"
     CDS             complement(1140..1436)
                     /locus_tag="ACHJWB_002923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314518.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            1720..3351
                     /gene="oppA"
                     /locus_tag="ACHJWB_002924"
     CDS             1720..3351
                     /gene="oppA"
                     /locus_tag="ACHJWB_002924"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4314519.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3437..4357
                     /gene="oppB"
                     /locus_tag="ACHJWB_002925"
     CDS             3437..4357
                     /gene="oppB"
                     /locus_tag="ACHJWB_002925"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4314520.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            4372..5280
                     /gene="oppC"
                     /locus_tag="ACHJWB_002926"
     CDS             4372..5280
                     /gene="oppC"
                     /locus_tag="ACHJWB_002926"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4314521.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            5292..6305
                     /gene="oppD"
                     /locus_tag="ACHJWB_002927"
     CDS             5292..6305
                     /gene="oppD"
                     /locus_tag="ACHJWB_002927"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4314522.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPCRLRACFKPVE
                     ELL"
     gene            6302..7306
                     /gene="oppF"
                     /locus_tag="ACHJWB_002928"
     CDS             6302..7306
                     /gene="oppF"
                     /locus_tag="ACHJWB_002928"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4314523.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7359..7688)
                     /gene="yciU"
                     /locus_tag="ACHJWB_002929"
     CDS             complement(7359..7688)
                     /gene="yciU"
                     /locus_tag="ACHJWB_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4314524.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7723..9183)
                     /gene="cls"
                     /locus_tag="ACHJWB_002930"
     CDS             complement(7723..9183)
                     /gene="cls"
                     /locus_tag="ACHJWB_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4314525.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9326..9499
                     /gene="yciY"
                     /locus_tag="ACHJWB_002931"
     CDS             9326..9499
                     /gene="yciY"
                     /locus_tag="ACHJWB_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4314526.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(9554..10807)
                     /gene="kch"
                     /locus_tag="ACHJWB_002932"
     CDS             complement(9554..10807)
                     /gene="kch"
                     /locus_tag="ACHJWB_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4314527.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(11106..11402)
                     /gene="yciI"
                     /locus_tag="ACHJWB_002933"
     CDS             complement(11106..11402)
                     /gene="yciI"
                     /locus_tag="ACHJWB_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4314528.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            11626..12345
                     /gene="tonB"
                     /locus_tag="ACHJWB_002934"
     CDS             11626..12345
                     /gene="tonB"
                     /locus_tag="ACHJWB_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4314529.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(12385..12783)
                     /gene="yciA"
                     /locus_tag="ACHJWB_002935"
     CDS             complement(12385..12783)
                     /gene="yciA"
                     /locus_tag="ACHJWB_002935"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4314530.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(12888..13427)
                     /gene="yciB"
                     /locus_tag="ACHJWB_002936"
     CDS             complement(12888..13427)
                     /gene="yciB"
                     /locus_tag="ACHJWB_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4314531.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(13457..14200)
                     /gene="yciC"
                     /locus_tag="ACHJWB_002937"
     CDS             complement(13457..14200)
                     /gene="yciC"
                     /locus_tag="ACHJWB_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4314532.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(14228..14317)
                     /locus_tag="ACHJWB_002938"
                     /pseudo
     CDS             complement(14228..14317)
                     /locus_tag="ACHJWB_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            14557..15195
                     /gene="ompW"
                     /locus_tag="ACHJWB_002939"
     CDS             14557..15195
                     /gene="ompW"
                     /locus_tag="ACHJWB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4314533.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(15255..15761)
                     /gene="yciE"
                     /locus_tag="ACHJWB_002940"
     CDS             complement(15255..15761)
                     /gene="yciE"
                     /locus_tag="ACHJWB_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4314534.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(15807..16307)
                     /gene="yciF"
                     /locus_tag="ACHJWB_002941"
     CDS             complement(15807..16307)
                     /gene="yciF"
                     /locus_tag="ACHJWB_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4314535.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(16393..16572)
                     /gene="yciG"
                     /locus_tag="ACHJWB_002942"
     CDS             complement(16393..16572)
                     /gene="yciG"
                     /locus_tag="ACHJWB_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4314536.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4424 a         3858 c         4002 g         4402 t
ORIGIN      
        1 tttaaacatt agagttatta tctctaatgc gtcacttcca ggtggcgtaa gcaagattac
       61 tcacttctgg gtactgatta cgtgatccaa atcaaatttt tgcaaagctg acacctttca
      121 gcatcgcttt tcgccattat agctaacagt taataaattg tagtatgatt tggtggctac
      181 attagcatgt tttgcacaac tagataacaa taacgaatga tagcaatttt aagtagttag
      241 gaggtgaaaa atgctgtcaa aaggcgtatt gtcagcgcgt cttttcaacc ttatttatgg
      301 ctaacattat ccggcttttg cttcggagct aaccgtgatt cagacctttt ttgattttcc
      361 cgtttacttc aaatttttca tcgggttatt tgcgctggtc aacccggtag ggattattcc
      421 cgtctttatc agcatgacca gttatcagac agcggcagcg cgaaacaaaa ctaaccttac
      481 agccaacctg tctgtggcca ttatcttgtg gatctcgctt tttctcggcg acacgattct
      541 gcaacttttt ggtatatcaa ttgattcgtt ccgtatcgcc gggggcatcc tggtggtgac
      601 gatagcgatg tcgatgatca gcggcaagct tggcgaggat aaacagaaca agcaagaaaa
      661 atcagaaacc gcggtacgtg aaagcattgg tgtggtgcca ttggcgttgc cgttgatggc
      721 ggggccaggg gcgatcagtt ctaccatcgt ctggggtacg cgttatcaca gcattagcta
      781 tctgtttggt ttctttgtgg ctattgcatt gttcgcttta tgttgctggg gattgttccg
      841 catggcaccg tggctggtac gggttttacg ccagaccggc atcaacgtga ttacgcgtat
      901 tatggggcta ttgctgatgg cattggggat tgaatttatc gttactggta ttaaggggat
      961 tttccccggc ctgcttaatt aattcctttc aaatgaaacg gagctgccat gctccgttta
     1021 cttcgtcatt atttttactt tgttcccgcg cagttatcaa aagcaaaagg aataggtaaa
     1081 aatattcttc tcaaattaca gttagttata aggatttcct taactgcttc tcctcaccat
     1141 catgttattt tcgccacatc ataatcctgg gcttgctgaa gaataattga aatgatatta
     1201 gtaattccac tgcctttggt agaggaaagt gctaaataat aatcaattgt taaattattg
     1261 tgcatttcac tactggaact gtaatcagaa aagatagaca tgcttagcca atctctattt
     1321 gattgaattg aaagatgttt gttaaggcat ggatgcaagc tatagattct gatacggtca
     1381 ataaaagaga attgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg
     1441 tactttccgg gcaaccgcca gacgattctt tattggtaat gagaataatt aacaattaaa
     1501 gagcgtcgcg aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg
     1561 tgatcgtctg aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta
     1621 acctgacagc agaaagtctc cgagcctgtg cagggtccca atccgggatt acacatgctg
     1681 gttaatacca gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga
     1741 agtttagtag cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct
     1801 gatgtacccg caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca
     1861 gaagttcagt cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga
     1921 gacctgtttg aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct
     1981 gaatcctggg ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa
     2041 tggtctgatg gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt
     2101 gatccgaaca ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt
     2161 gatgaaattc ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat
     2221 cacacattag aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac
     2281 ccatcaactt caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag
     2341 cctggtaata tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga
     2401 atcgttcttg aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta
     2461 acctatttgc ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc
     2521 gacatgactt ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg
     2581 gacgaagttc acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa
     2641 ccgccattca acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc
     2701 attgttaata aagtgaaagc gcagggcgac atgcccgcct atggttacac tccaccgtat
     2761 actgatggcg caaaattgac tcagccggaa tggtttggct ggagccagga aaaacgtaac
     2821 gaagaagcga aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc
     2881 aacctgttgt ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg
     2941 tggaagaaaa acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc
     3001 gacacccgtc accagggtac ttttgatgtg gcccgtgcag gctggtgtgc tgactacaac
     3061 gaaccaactt ccttcctgaa caccatgctt tcgaacagct cgatgaatac cgcgcattat
     3121 aagagcccgg cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag
     3181 cgcacagctc tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct
     3241 gtttattact acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa
     3301 gatccgctgg ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac
     3361 gtggggcagg agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag
     3421 gccagaaggt agggcaatgt taaaatttat tctacgtcgc tgtctggaag cgattccgac
     3481 gctatttatt cttattacta tttcgttctt tatgatgcgc ctcgcgccgg gaagcccttt
     3541 taccggcgaa cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct
     3601 taatgatccg atcatgacac agtatttcag ctacctgaaa caactggcgc acggtgattt
     3661 cggtccatcg tttaaatata aagattattc ggtcaatgac ttggttgcat ccagttttcc
     3721 cgtttctgcc aaactgggag ccgcagcatt tttccttgcg gtaatactgg gtgttagtgc
     3781 tggcgttatt gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc
     3841 aatgaccggg gttgttatcc ccagttttgt ggttgcacca ttattagtca tgatatttgc
     3901 gatcattttg cattggctgc cgggcggtgg ctggaatggt ggggcgctta aattcatgat
     3961 attgccgatg gtggcgttgt cactcgctta tatcgccagt attgcgcgta ttacccgtgg
     4021 ctctatgatt gaagtattac actccaattt tattcgtact gcccgggcga aagggttacc
     4081 tatgcggcgg atcattttac gtcacgcatt aaaacctgct ctgttacccg tgctctctta
     4141 tatgggcccc gcatttgtcg gcattattac cggttctatg gttatcgaaa ccatttatgg
     4201 tttgccgggg attggacaac tgttcgttaa tggcgcattg aaccgtgact attccttagt
     4261 gttaagcctg accatcctgg ttggtgcttt aaccattttg tttaatgcca ttgtcgatgt
     4321 gctatatgcg gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta
     4381 agtaagaaaa acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc
     4441 agcttgtggc aggacgcacg tcgacgtttt attcataacc gtgcggcggt tgccagtctg
     4501 atagtgctgg tgctgatcgc gttatttgta atcctggcac cgatgctttc ccagtttgcc
     4561 tatgacgata ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac
     4621 tttggtactg actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg tgggcgtatc
     4681 tcactcatgg tcggtgttgc tgcggcactg gtggctgtgg tcgtcgggac actttatggt
     4741 tcgctttccg gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc
     4801 ctcaactcct tcccattcat gttcttcgtc attttgctgg tgaccttttt cggtcaaaac
     4861 atcctgctga ttttcgtggc gataggcatg gtttcctggc tggatatggc tcgtattgtg
     4921 cgtgggcaaa ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt
     4981 gtatcgacat cgggcattgt tattcgccac attgtgccga atgtactcgg tgtggtggtg
     5041 gtctacgcat cacttctggt gcccagcatg atcctctttg aatctttcct tagcttcctg
     5101 gggttgggta cgcaagagcc gttaagcagc tggggtgcat tgctgagtga tggcgcgaac
     5161 tcgatggaag tctctccatg gttattgttg ttcccagcgg gattcctcgt ggtgacgctg
     5221 ttttgtttca actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa
     5281 ggagtgcagc catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg
     5341 acgcactgct gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca
     5401 cggcggtcaa tgatttgaat ttttccctac gtgccggaga aacgctgggt attgtaggtg
     5461 agtctggttc gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg
     5521 gacgtattgg cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcatg
     5581 aactcaataa actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt
     5641 tgaatcccta tatgcgcgtc ggtgagcagt taatggaagt gctgatgctg cataagaaca
     5701 tgagcaaagc tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg
     5761 aagcgcgtaa acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag
     5821 tcatgattgc gatggcattg ctatgtcgac ctaagctact gattgcggat gaacccacta
     5881 ctgcgctgga cgtcaccgta caggcgcaga tcatgacgct attaaatgaa ctgaagcggg
     5941 aatttaatac cgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgcg
     6001 acaaagtgct ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct
     6061 tttatcaacc cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg
     6121 cggaaggtga aacaatgttg accatccctg gtaatccgcc aaacttgctg cgattaccga
     6181 aaggttgccc gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc
     6241 cgctggaaga gtttacgcct tgccgtctgc gtgcttgctt taaaccggtg gaggaactgt
     6301 tatgaatgct gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca
     6361 ctttgaaatc aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt
     6421 cgatggtgtc actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg
     6481 atgcggtaag tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca
     6541 tgttgcctgg ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg
     6601 cagtgatatt cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat
     6661 cggcgagatc atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt
     6721 tcgcgagcgc gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg
     6781 ctatccgcat gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat
     6841 tcttgaaccg aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca
     6901 ggcgcaggtg gtcaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt
     6961 tatcgctcat gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct
     7021 cggccatgcg gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta
     7081 caccagggca ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac
     7141 catccagtta ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt
     7201 ccgtacccgt tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg
     7261 gagtttcaga cacgccgttt cttgcctgaa agtcgatccg ctttaaacac aataagggct
     7321 gacagttgtc agcccttttt cacgccgaaa gcgattattt attcccgcca gatgatatgg
     7381 caaagtttgt gatctttttc gcggcataac agaatgcggg cgaaaacatc gttgatttca
     7441 ccatcttcac tgtccgccag accaatcacc acttcggcaa aaaagtctgg gttcaaatcg
     7501 aaatcaacat gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct
     7561 tcaaactgaa gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg
     7621 agaaaaatat cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc
     7681 atatccatag acactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa
     7741 gaagtaaaac agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc
     7801 atcgagcaga cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa
     7861 acctttatcg tcgattgcca gggtaatctc gaaattcagc cacagactac gcatatcaag
     7921 gttaacggtg ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa
     7981 cccgccttca aactgataaa ttttaacccc agcagcaaga agttccgtaa agaatgcgcg
     8041 actggcccag ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac
     8101 cccgcgctgc gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg
     8161 cgtggtcatg atcaaatatt cacgcgccga ataagccgca gtcaataatg cctggtgaat
     8221 gagatcttcc ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg
     8281 ttcaaacggc ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat
     8341 ctcccagtcg caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc
     8401 catcagatca atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat
     8461 attcatgctg ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg
     8521 caggtccata cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc
     8581 aataccggca ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc
     8641 ggagtcgagc atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc
     8701 cacctggtcc gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg
     8761 cgcgagctgg atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagttg
     8821 tagctgattc cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg
     8881 cgcagcgaca ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag
     8941 ccattttgcg gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg
     9001 gagctcgcca acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa
     9061 cagccaggcc atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc
     9121 tgcaatgagc aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt
     9181 catagattag aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct
     9241 ggcaaataaa aacgcgggaa aagcactggt cagcgtcggg ggtgggggta taatgaccat
     9301 tctgttattg catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg
     9361 catgcagcgt caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa
     9421 tatgcgtatc cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt
     9481 tgcgatctac aatatctaaa tatactgggc accattgcgg tgcccaataa gtttactctt
     9541 tcgtctttaa actctatttt tgcactgatt ctttactgtc agctttcgtt tcttgttcat
     9601 cgttatcgct gaaaatgcca tgaccggagt tcaacaacat tgaaacgagc atatcattat
     9661 taatctcttc accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga
     9721 gaatgatatc cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga
     9781 gaactgtttt gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg
     9841 catcgttatc actcagcgcc agaatggccc ggcaacgatc gattcccgct ttctttaata
     9901 ctgaactgtc attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa
     9961 gttgcttgat atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat
    10021 tcagttgcag gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt
    10081 tacgatgcat tgtatgattg tttcctttta caagtttgtt gaatcccccg cggataagcg
    10141 gaccaaaaat tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa
    10201 tagtgaacaa tcgtgctgat tcagaaacag ggacaatatc gccatagccg acggttgaca
    10261 tggtttctat cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt
    10321 cgcttaaata aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg
    10381 caaaaattgt cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa
    10441 acaccagcgt aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg
    10501 tgtaaattag cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga
    10561 acagtaagcc aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg
    10621 ccagcaagga gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga
    10681 ccgacatacc atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga
    10741 gaatgtcgtg ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt
    10801 gactcactga tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct
    10861 gaacatacat gcagcgcgtg aatgtgttaa acaatagtag atgactttat cggcagtaat
    10921 caaaggtttt gagcgagcaa ctctgcgtca ggaaagggca ttttcctgaa tatgttcaca
    10981 tcatataaag aaatacagat aaagttatta tctgcttgtg gtggtgaatg cactgaccgg
    11041 ctataaggaa aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattgaatc
    11101 tctattcaga acactttctt aaatggtttc actgaaacgt gttcatagac tcctgccgct
    11161 acgtacgggt cagcatcggc ccaggcctga gctgcttcca gcgattcaaa ttcagcaata
    11221 acggttgagc cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga
    11281 ccagctgtca acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga
    11341 cgaacggaaa ggcgtttttc gaagctatca gctttgtctt gagcgtaaat aacatacaac
    11401 acgggcacaa ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc
    11461 tgcaacggaa agatgatgtc tttgttaaga ccatgcataa agtaaggtca attacgccaa
    11521 aaatgacatt ttcactgatc ctgattgtct tgccttattg aatatgattg ctatttgcat
    11581 ttaaaatcga gacctggttt ttctactgaa atgattatga cttcaatgac ccttgattta
    11641 cctcgccgct tcccctggcc gacgttactt tcggtctgca ttcatggtgc tgttgtggcg
    11701 ggtctgctct atacctcggt acatcaggtt attgaactac ctgcgcctgc gcagccaatt
    11761 tctgtcacga tggttgcgcc tgctgatctc gaaccgccac aagccgttca gccgccaccg
    11821 gagccggtgg tagagccaga accggaacct gagccgatcc ccgaaccgcc aaaagaagca
    11881 ccggtggtca ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag
    11941 gtacaggagc agccaaaacg cgatatcaaa cccgtagagt cgcgtccggc atcaccgttt
    12001 gaaaatacgg cacccgcgcg ccccacatca agtacggcaa cggctgcaac cagcaagccg
    12061 gttaccagtg tggcttcagg accacgcgca ttaagccgta atcagccgca gtatccggca
    12121 cgagcacagg cattgcgcat tgaagggcag gttaaagtta aatttgacgt cacgccggat
    12181 ggtcgcgtgg ataacgtaca aatcctctca gccaagcctg cgaacatgtt tgagcgtgag
    12241 gtgaaaaatg cgatgcgcag atggcgttat gagccgggta agccaggcag tgggattgtg
    12301 gtgaatatcc tgtttaaaat taacggcacc accgaaattc agtaagcaga aagtcaaaag
    12361 cctccgaccg gaggcttttg actattactc aacaggtaag gcgcgaggtt ttccttcagg
    12421 atcaaccgcg acatacttaa ataatgcttc tgtcgcttta tagcgttgcc cgattggttc
    12481 agacgctact tttttcaccc acacttcaat attaatgctg accgatgtcg tccctttctg
    12541 gacacagcgt gcatagcagc acaccacatc gccgaccgca accggccgta agaaagtcat
    12601 tccttcaacc cgcacagtca ctacgcgacc gtgggcaatt tctttcgcca gaatagcgcc
    12661 gccaatatcc atttgtgaca ttaaccaacc accaaagatg tcaccattgg cattggtatc
    12721 ggcgggcatg gctaaagtac gtaaaacaag atcgccctga gggacgttat gtgttgtaga
    12781 catggtaaaa ccgacttaaa aggaaaatca caggcgcgat gctactatga ttttcctgag
    12841 gagaacagag gagatacagc gccagccccg aaggactggc agtctggtta ggatttatct
    12901 tcctgcggca tgtggcggta gatatagata ccgcttaaca atgtgaagat taaggtaagg
    12961 gcggtcaggc caaagacttt aaagttgacc caaatatttt gcggcagcca gaatgcgatg
    13021 tagatgtttg ccagaccgca aaggataaag aaaacagccc aggccagatt tagcttcgac
    13081 cataccggtt gcggcagcgt gagttcttta cccagcatcc gctgaattag cggctttttc
    13141 atcacccatt ggctgactaa cagggctccc gcaaacagag cataaatgac tgtaaccttc
    13201 catttaataa actcatcatt gtggaagaac agcgtcaagc caccgaagac gaccaccaga
    13261 acaaaagtga tcagggccat cttctcaacc ttacgaaagc gaacccagct atagataagc
    13321 acaatcgccg tggcgacgat cagcgccgca gtagccgcat agatgtcata aatcttgtaa
    13381 aacgcgaaaa agacaaccag cggtaaaaaa tcaagaaact gcttcatttt acgattccgt
    13441 aatcaaatgc ttaaggttat tggcgaatca acatatacag gcggaacaga tagatgagca
    13501 atatggcaga aatcaggttg ctcaaggtgt tcgccagtac ggcaccaatt tccggggtta
    13561 atgcggcaaa agaagaggca aaaagcagca acagtgtttt tgccagcaac cagctcagta
    13621 ctgcgggtgc caccagacgc atattcgccc aagtcagccg catactgcta cgcatcgagg
    13681 caaaaatgcc cattttgtcc tgtaccagca tcaccggtgc cagggccagt aaaatggcca
    13741 tgataattcc cggaacgacc accagcatga tgccaatctg tactaaaagg gtagtcagaa
    13801 aaatcagaat aaataacttg ggcaatatcg gcgcactggc accaatagct cgcagcgcac
    13861 tgactctctg acccgcagac accagctgga taattaatat tacgcctccg gcgagaatgg
    13921 cgttaccgat taatcctgaa aaagtggacg ccgctgaagc ctgcagcaaa atttgttgct
    13981 gttccggtga catattctga accaggtcga acaacccact actgccgcta acgggcacgc
    14041 cgtcattgag ctgcgcaagc tgtgcatcac tgggtgagaa aacatgccct aacaccactg
    14101 tgataaacgc acatagcaac gataccaaca gaatggtcat aaattgatta cggaagaaat
    14161 ttccggtgtc acggtatacg gactgcgccg tgatagacat gcactctcct tgagttttat
    14221 gcaggtgtta attatcgggc aattgtaccc tggttaagcc tcgggtggca gcatcaggtc
    14281 ttgtatggaa aagcatatct ttgtaaagcg gaggtaattg ctggccttca ggcgtgggca
    14341 aaccgcggca agtggcgatc gagttaatat ttgcgtagag aaaatattta aaaattgatt
    14401 taaatcacat taaccaggat tctcaatgca acttctaaat taatccagat caataaatgg
    14461 tgaattatca tatgtaatgt gatctatgta ggatcatttg ttactccaat gtaggtatat
    14521 tcgtcacgtt tttataacca taacgacgga gcggatatga aaaagttaac agtggcggct
    14581 ttggcagtaa caactcttct ttctggcagt gcctttgcgc atgaagcagg cgaatttttt
    14641 atgcgtgcag gttctgcaac cgtacgtcca acagaaggtg ctggtggtac gttaggaagt
    14701 ctgggtggat tcagcgtgac caataacact caactgggct tgacgtttac ttatatggcg
    14761 accgacaaca ttggtgtgga attactggca gcgacgccgt tccgccataa aatcggcacc
    14821 cgggcgaccg gcgatattgc aaccgttcac catctgccgc caacactgat ggcgcagtgg
    14881 tattttggtg atgccagcag caaattccgt ccttacgttg gggccggtat taactacacc
    14941 accttctttg ataatggatt taacgatcat ggcaaagagg cggggctttc cgatctcagt
    15001 ctgaaagatt cctggggagc tgccgggcag gtgggggttg attatctgat taaccgtgac
    15061 tggttggtta acatgtcagt gtggtacatg gatatcgata ccaccgccaa ttataagcta
    15121 ggcggtgcac agcaacacga tagcgtacgc ctcgatccgt gggtgtttat gttctcagca
    15181 ggatatcgtt tttaattccg cacaaaaacg accccgtaat atacggggtc aataaggaca
    15241 tggtataaag ggtattattt cttcgcttct acgccatcag tttcagaacg aattaaaaat
    15301 ttctcagttg tttgcggaat atgctgaatc agccagtcgg ccatttgctt ttcctcattt
    15361 aaaatcgctt cgatgatcgg aattgagact gtatcaccgg cattttttgc tgctgctaat
    15421 agtgaggtgt aacaggcgat ttcaaattgc tcgaagacat atccgctaat agagccttta
    15481 actatttcat cagaagggaa tataccaccg attgactgcc caagcgcagc cattttactc
    15541 atggaatctt taatgactga acgtgaaatg tcattacgat caagaatagt ttccagttga
    15601 acaatctggt ttttggtttc actaagatgt tgttcaatac gagcgcgtag ttcaggataa
    15661 ttatctatac ggctggccat ggattcaagc atagattcgg cttgcttttc cattgcgtgg
    15721 gcgtcacgta accagtcatg ataatgttca atacgattca tgaaaatgtc ctctttataa
    15781 aatgactaaa agttaaaatt catatttcag gctttatttt cggctttctt atttacgtta
    15841 ttaagggcca gatcagtcag tttgatgtcg gtggcctttt cttcttccag ggtttctttc
    15901 agaagcttcg ctgctttacg gtaacctaat tgttcagcca gcgtcgctaa tgtcccgtaa
    15961 ctggcaatct cataatgctc gactttctgt gctgcggcaa tcagtgcggc atcacgcact
    16021 tcgtttttct cggtactttc gatgacctca ttagcttctt caataagacc ttccattgcc
    16081 acacatttca tgcgcttaat tttcaggttc gattcggact ccacaacttg atcaatacgt
    16141 tcaatctgtc catgagtttc ctcgaggtgc gcatgaaaag cctgacttaa tttttcattt
    16201 gatgttgctc ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc gctgtaggta
    16261 tctgaaagca ggtgaataaa tacatcttca atggtcttca tattcatatt tttctccagt
    16321 gaaatcactg cgagccatac ggcccgcaga tgcaattatc tttatcagct gatattaata
    16381 attaacggtg aatcaggatt tgcctgattt attaccaccg ctttgttgac cgcctttttt
    16441 acccgcttca gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat gctgaccgcc
    16501 tttacggcct gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg aaccaccacg
    16561 atgttcggcc atgttatttc tcccgttgcg ttgcattgtt tcattaatat gagtgttgtg
    16621 tgtcgacact cattaaaatt agtcgctaat gaggattagt caaattaagc gcaacgagaa
    16681 gataga
//
LOCUS       DAZFAH010000099        16521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFAH010000099 DAZFAH010000000
VERSION     DAZFAH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.099"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..191)
                     /locus_tag="ACHJWB_002943"
     CDS             complement(39..191)
                     /locus_tag="ACHJWB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4314537.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(456..1190)
                     /gene="cysH"
                     /locus_tag="ACHJWB_002944"
     CDS             complement(456..1190)
                     /gene="cysH"
                     /locus_tag="ACHJWB_002944"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4314538.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHALKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1264..2976)
                     /gene="cysI"
                     /locus_tag="ACHJWB_002945"
     CDS             complement(1264..2976)
                     /gene="cysI"
                     /locus_tag="ACHJWB_002945"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4314539.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2976..4775)
                     /gene="cysJ"
                     /locus_tag="ACHJWB_002946"
     CDS             complement(2976..4775)
                     /gene="cysJ"
                     /locus_tag="ACHJWB_002946"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4314540.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5094..5456
                     /gene="queD"
                     /locus_tag="ACHJWB_002947"
     CDS             5094..5456
                     /gene="queD"
                     /locus_tag="ACHJWB_002947"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4314541.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5534..6805
                     /gene="ygcN"
                     /locus_tag="ACHJWB_002948"
     CDS             5534..6805
                     /gene="ygcN"
                     /locus_tag="ACHJWB_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4314542.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAAFLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6796..7056
                     /gene="ygcO"
                     /locus_tag="ACHJWB_002949"
     CDS             6796..7056
                     /gene="ygcO"
                     /locus_tag="ACHJWB_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4314543.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7073..7648
                     /gene="ygcP"
                     /locus_tag="ACHJWB_002950"
     CDS             7073..7648
                     /gene="ygcP"
                     /locus_tag="ACHJWB_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4314544.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7796..8655)
                     /gene="ygcQ"
                     /locus_tag="ACHJWB_002951"
                     /pseudo
     CDS             complement(7796..8655)
                     /gene="ygcQ"
                     /locus_tag="ACHJWB_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(8652..9431)
                     /gene="ygcR"
                     /locus_tag="ACHJWB_002952"
     CDS             complement(8652..9431)
                     /gene="ygcR"
                     /locus_tag="ACHJWB_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4314545.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9409..10818)
                     /gene="ygcS"
                     /locus_tag="ACHJWB_002953"
     CDS             complement(9409..10818)
                     /gene="ygcS"
                     /locus_tag="ACHJWB_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4314546.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10840..12294)
                     /gene="ygcU"
                     /locus_tag="ACHJWB_002954"
     CDS             complement(10840..12294)
                     /gene="ygcU"
                     /locus_tag="ACHJWB_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4314547.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12364..13149)
                     /gene="ygcW"
                     /locus_tag="ACHJWB_002955"
     CDS             complement(12364..13149)
                     /gene="ygcW"
                     /locus_tag="ACHJWB_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4314548.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLIGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13468..14745
                     /gene="yqcE"
                     /locus_tag="ACHJWB_002956"
     CDS             13468..14745
                     /gene="yqcE"
                     /locus_tag="ACHJWB_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4314549.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGIFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14772..16250
                     /gene="ygcE"
                     /locus_tag="ACHJWB_002957"
     CDS             14772..16250
                     /gene="ygcE"
                     /locus_tag="ACHJWB_002957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4314550.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3920 a         4344 c         4162 g         4095 t
ORIGIN      
        1 cacaattcag tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg
       61 agaactgcct taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct
      121 accatcagcg tgaatcccag tactgtaaaa cacagcacta tgattgccac aagggcatat
      181 tttgtcagca tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc
      241 gaacgctggc ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg
      301 cgaatgctta acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt
      361 ccagcggcgt tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca
      421 tttctgacag aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc
      481 ctttttaagc caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt
      541 gtatgggtat caccgaccga taaatatcct tcatcccata atgggtgata tttcagggca
      601 tgtttttgca ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta
      661 aatacgccac gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg
      721 cgcaggccag caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg
      781 actttgttga tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagttttccg
      841 tagcgtgctt cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg
      901 agtttgtccg ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtgtcg
      961 gtgaggatca ccggaatatc cgggtgaatc tgattcacca gatgcaggct caccgccgcc
     1021 tgaatgccaa agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggca
     1081 acgcggcctt cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg
     1141 cgatctacct tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca
     1201 ctgtttgcct tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga
     1261 tggttaatcc cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac
     1321 cgtaaagtcg ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc
     1381 atcaagcgac gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg
     1441 tgtcccaatg cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag
     1501 gcccacttcc gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac
     1561 gatatgctca tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga
     1621 cggcaggaaa cgctctgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc
     1681 catcgagttt tcacgctgcg gcgtgacggc attcattaaa ccgctctctt tggcgatctt
     1741 ctcgatcttc gctttctcgc tttccggtac accggcgatg atcagattct ggttcgccgt
     1801 aatgcggaaa tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg
     1861 ccccggataa tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc
     1921 aatgccctta acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg
     1981 ttcaaatttg atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg
     2041 ctccagcgtg tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg
     2101 agttgtcacg acggcttcgg ccaccgccag cgtatgctcc agcggcagat agccaaactc
     2161 gctcgccgtg cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac
     2221 cagcaggttg aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt
     2281 ggcatgcaga tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg
     2341 caggtaggtc tgacctagga tcggttcttc atcagtagtg gcgacttttt cctggtcgag
     2401 ccagatctcc gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca
     2461 ctcgtacgct tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg
     2521 gttcatgtcg ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac
     2581 cggtttgacg ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg
     2641 aatgctgcca tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt
     2701 ggtaatcacc ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt
     2761 ctgttcagca cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg
     2821 gatcagcagg aagttatcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc
     2881 aatggtgccg cgtaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc
     2941 ttcgaccact aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg
     3001 cgctctacgc gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat
     3061 tcggcaatca cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg
     3121 cagacataaa tgtgggcacc atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc
     3181 agtttgtctt gtacgtaaac tttttctttt tggtcgcgcg accaggcaag atcgatacgt
     3241 gtcagcacgc catctttgac gtagcgctgc cactccacct ggtacaggaa gtcttccgta
     3301 aagtgcggat taccaaagaa cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc
     3361 tgcataaagg cgcggaacgg cgcaataccg gtgcctgggc caatcataat caccggggtt
     3421 tccgggttag cgggcaggcg aaagttatcg ttatgttcga taaatacgcg gacttcgccc
     3481 tcttcttcca cgcgatccgc gaggaagctg gaggcaccac cggcacgggc tcggccttcc
     3541 acgtcgtaac gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag
     3601 gaggcgatgg aatacaggcg cggggtcagc gggcgcagca gattaatcag cgcttcggca
     3661 tccagttgcg ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc
     3721 tgtaacttcg ctttatcgcc caccagcggc agcagtgttt cgctgcgggt aagcgtggcg
     3781 taattctcaa caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg
     3841 ttcagaggca acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt
     3901 tcgacaagtt ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc
     3961 ggctggtaac gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca
     4021 gagttacgtc cggtaatttt ctggttaacc gaaaggctcg ccaccagcgg agcgtctttg
     4081 ctgtacgggc tggagtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc
     4141 gcgacaggcg cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca
     4201 gcctggtatt caacatcggc atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc
     4261 agcttgctgt caaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg
     4321 ctaaacacgg caaatgcggt gttttccagc ttcggcgctt ttttggagaa caggaactta
     4381 tgcagcgcga cggcttcttc cggcggttcc ccttctcctt gcgttgacgt caccacgata
     4441 agcagttttt cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg
     4501 ttcagttttg ctgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg
     4561 gtttgcgagg cggagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct
     4621 gcaagcgcag caggctgctg attgagtacg ccccagaaat agccagaaac ccaggcaagc
     4681 tgggtaggcg ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac
     4741 ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa
     4801 gctgtttctg cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggtg
     4861 aacggtgtgg attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt
     4921 agcaatagtc tttaacaaca aaatagatta accaacctaa tgaaaaacaa atgaatttag
     4981 ccaatcatta agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa
     5041 aacagccttt ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca
     5101 ccacgttatt taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag
     5161 ggcataaatg tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg
     5221 aagtcgatcc gcatacgggc tggattatcg atttcgctga actaaaagcg gcgtttaagc
     5281 caacttatga gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa
     5341 ccagcgaagt tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa
     5401 gtgcggtgat ggtaaaagaa acctgcaccg ctggttgtat ctatcgcggc gaatgataag
     5461 agtgtgtcgg cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata
     5521 tctggggcat gacatggaag acgactgcga cattattatt attggtgccg gtattgcagg
     5581 caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc
     5641 tgaaatcccc ggcagcaaaa atctttccgg cgggcggtta tatacccatg cactcgcgga
     5701 actcctcccg caatttcatc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct
     5761 ttccctgtta acgccggatg gcgcaacgac gttttccagc ttacagcccg gcggtgaatc
     5821 ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aagaagaagg
     5881 tgtcgaatgc atccccggag cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg
     5941 tggtgtcatt tgtggtgacg atattctccg tgcccgttat gtggtgctgg cagaaggtgc
     6001 caacagcgtc ctggctgagc gtcacgggtt agtgactcgt cctgctggtg aagcgatggc
     6061 gttggggatc aaagaagtgc tgtcgctgga accatccgct attgaagaac gttttcatct
     6121 ggagaataac gaaggcgcag cgtttctgtt cagcggcagg atctgtgatg acttacccgg
     6181 cggcgcattt ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc
     6241 ttcccttacg caaagtcgtg ttccggcaag cgagctgctg actcgcttta aagcgcatcc
     6301 ggcagtgcgc ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc
     6361 agaaggtggc ttgcacagta tgccggtgca atacgccggt aacggctggc tgctggtggg
     6421 cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct
     6481 gactggcgcg caggcggcgg cacaaacgct gataagcgcc tgccagcacc gcgagccgca
     6541 aaatctgttt gcgctttatc atcacaacgt cgagcgcagc ctgctatggg atattctaca
     6601 gcgttatcag catgttccgg cgcttttgca acgccctggc tggtatcggg cgtggcctgc
     6661 gttaatgcag gatatttccc gcgatttatg ggatcagggt gataaacctg ttccaccgct
     6721 gcgccagtta ttctggcgtc atttacgtcg tcatggcctg tggcatctgg cgggcgatgt
     6781 tatcaggagt ctgcgatgtc tgtagcccat aatctctggc gcgttgctga tgcgccgcac
     6841 attgttccgg ctgactccgt tgagcgtcag atggtacaac ggttgattaa cgcctgtccg
     6901 gcaggtcttt tttcgctcac accggaaggt gacttacgcg ttgattatca cggctgcctg
     6961 gagtgtggca cctgccgttt gctgtgcgac gaatccacac tacaacagtg gcgctatccg
     7021 ccttccggat tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct
     7081 cttacacctg ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca
     7141 actggctatc gattctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat
     7201 cagtaatatc gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt
     7261 gctggaaggc gcgtcaaata aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc
     7321 ggacggcatt atcagtacca aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt
     7381 ctgtattcat cgcctgttta ttgttgattc aatttcgttt cacaacattg ataagcaagt
     7441 tgcgcaatcg aatccggatt gtattgagat cctgccaggc tgtatgccca aagtgctggg
     7501 ctgggtgaca gagaaaatcc gccaaccgct gattgccggt gggctggtgt gcgatgaaga
     7561 agatgcgcgt aatgcgatta acgcgggtgt cgtggcgctt tccaccacga ataccggggt
     7621 ctggacgtta gcgaaaaaat tactttgacg ggataatcgt aaccaattga atttggtttg
     7681 atttgtaggc cgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt
     7741 atcaggccta caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt
     7801 ggcaatctgc gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca
     7861 cgccaacatc tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa
     7921 acttgctatt gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac
     7981 acacctcagg tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac
     8041 cgttcatcac tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag
     8101 cgatttcctg gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag
     8161 ctaatgggtc gcgggtaaca tttttcagat cctctgtgct aaccagccag tccggaagcg
     8221 cacccgggac aatgttcagt tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc
     8281 ccgcctgacg cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta
     8341 gcgcatttcc ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct
     8401 ggcagatgct tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg
     8461 ttcccggcgg aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat
     8521 ccagcacctg ttccgccact acaggcgagg ttcaatctgc cagtgcgcca gggtacaacc
     8581 gctgaaatcc tgcgccgcca gccagcttgc tattgcggca ttctcctgat ttattgtcac
     8641 aattgcgata ttcatggctg catcctttgg cgtaaatagt cccgccatag cttttgagct
     8701 ttttctgcta ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc
     8761 agttgcagac actgcatcgc tggcatttcg gcagcaacag ttttacggat aatttctgct
     8821 ttccccgcag ccattcgctg gcgcattccc ggtactggca aggccacttc accgcactga
     8881 cgcacggcaa tcaccgcagg caagcgaacc cggcaacaac gcaacccatg ttcagtacgc
     8941 tgttcgaggg tgataaacag cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc
     9001 cagcccagca tttccgccag caaaaatggc gtttgcccat tctgcccttc gctgctttgg
     9061 cagccggtaa ttatcagatc cagcggattc tgatgctgcc attcggcaat atggcgagcg
     9121 acaaattccg gcgcaaagcg cagatctgcc gccgtctcca gcaatacagc ttcctcaaac
     9181 ccaagagcca tgagatagcg cagccagtgc aatgcccgtt catcccccat gctcaacgcg
     9241 gttaaagaca tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc agcggcctgt
     9301 tcatcagcac cgagtaaact tcgcagtagc gaaatatccg gtccgctttt accctgagcc
     9361 gccgcctgcc actctttttc cgccagcatt ccggcatccg gttcggcttt aaacgctaac
     9421 agaatgttca ttcgcacctc ctacatttcc cgccgccacc agcgggaggg ctttagtttc
     9481 tggtgcccat agccaggtca caacaaaacc aaccaacaac actgtcgcca ggagcaataa
     9541 ggtgacttgc attccccact gcgccagcac ccacggcagc aggccagtac ttaccgccgc
     9601 gccaagtcga ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa
     9661 actttccgca ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg tgctgaagag
     9721 aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa caggccatta ctaccagcgt
     9781 tgccgccagc agcaaaaaac ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag
     9841 aactaatccc agcagcgcgc ccacaattaa caacgcatta agcatcaggc tggcagtcag
     9901 cgcatcttcc agaccaatag tctgggcgat agttggcagc caggtataaa tcacaaacca
     9961 tgggattacg aggcagacaa agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga
    10021 agagaacaag gttttgatgt gtttatgggt cgccgttacc acttcatcgc ccagtaaaac
    10081 atggggacca aaatagcgat gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag
    10141 tagccagcgt ggtgattctg gcgttcccca gcgtaataac gtaatcaaca acgcgggcag
    10201 agctgccgat gccagtagcc agcgccaggc ctccgggttt tcggaaataa agtgatgtcc
    10261 ggcaatactt gccagcacat agcctacggt ccacaccacg ctgaatgcgc ccagcaaaat
    10321 accgcgatgg cggcgcgggg aaaattcagc cagcaaggtg tgacctactg aataatcgcc
    10381 tcccagacca atgccaatca aaatgcgcag tccaataaga tgctctggcg tggtggcaaa
    10441 aaattgtaaa aacgaagcaa gcgtaatcag caaaaagctg aaggtgaaga ttttttgccg
    10501 accaatatgg tcggagatcc acccaagaac caggctgcca aggaacaaac caaggagcgc
    10561 cgagccgccg atcattcccg ccataaacgg cgtcagttgc atggcgggcg taagctgaat
    10621 aatggcgtaa ccaatgacgc cgagaacata accgtcggtc aggtgtgcgc cgaaagtgag
    10681 cgcagcaatg cggcagtgaa aacggttaag cggtaaatca tccattcgca ccggtgaagt
    10741 gttcatcttt accccttcat tgtccggtaa taagaacggc ttctcgcctg agaagccgaa
    10801 aaggcaacgc cccgtcatcg cggaacgctg cctgacacat tatttttcaa tcggatagat
    10861 agtacccgta ttcataatgc cattaggatc gaactgcttt ttcagccctt ccagcaacgc
    10921 ccacgcgctg ccgtgttcca gtttgctcca gtgaacgcga tgtttaccga taccgtggtg
    10981 gtgcaccatc gaaccgccga ggcgaatggt ttcttcacag atgatcttgt tgagcggatt
    11041 gtggtacttg tcgatttcct cttccggctt acagtcaacg acgttgtaat cgtagacgaa
    11101 gtacatgttg gtgccgttct gatagctatg agaggaatga ccgcccagca tggtgatgtc
    11161 gtcggcgtgc gggaactcag tacgaatacg gttaataacg ctttcgtaga tttcgtggat
    11221 gcagctccag cagccggaca cttcagtggt aaagcccatg ttgccggttt tgaggatctg
    11281 cacacgttcg gcagccactt tatccggtcc ccagttcagg ttgttaaacc aggtttcgat
    11341 cagtttgctg tccacgcgct ggcactgcgg gtagcgggca acgatttccg caatcccttc
    11401 gcccgtcgcc ttcgcaatgc gaggattgcc ttcagccata aagatcagca cacatttacc
    11461 gtcggcaaaa tgggtgaagt gttgggtgcc atcttcagcg tcatacaaac gagcgatcga
    11521 cggacgatac ccttccacca tgacttcacg caggatgttg aagccggttt tcatgtcttc
    11581 caggatatag ccgtagaaga ggttgttttc cggggtgaat ttaaagattt tcactgttac
    11641 ttcagtgata tagcaaagtg caccttcgtt accgatgatg atgtggcgaa tgtccgggcc
    11701 agccgcgcgg cgtggcacgt ttttaatgcg tgtgacggtg ccatcagcca atactgcttc
    11761 cagaccaacg accatatctt cgattgcgcc gtagagtgtg gagaactgcc cgatactgcg
    11821 ggttgctacc aggccgccca tctgcgccag cggctttgac tgcggagaat gccccgtggt
    11881 gtaacctttt tcacgcaacg cattttccag cacctccagc ggaacgccac attgcgccgt
    11941 cgcctgcata ttctcaatat caatattaat gatttgattc atggcggagc cgtcgagcac
    12001 caccgagttt tccacaacgg tttccagccc accttcagtg gcggaagcgc cggtacgcgg
    12061 cacaccgtta attttgtgcg cattcataaa attcagcaca cgggagactt gttctgtgga
    12121 accgagtttt acgaccgctg ccggaatcgg caaagtataa ataccatgaa tatccggaaa
    12181 tttacgaaaa cggtcaatac tgttcttctt taatactgtt tcatcggtaa ttacgcgatc
    12241 tgcaccaaca atttccttta gctggtcgac aatcgctgcg cgagataaag acatagtaat
    12301 tcattcctga taataaaaat ggaataatca ttaataagag aaaacagaaa ccaccgttat
    12361 ggattagcgc actaaataac caccatcgac cactaataaa tgcccattga cataatttga
    12421 agccggactt gcgaggaata cagctgcgcc tattaaatcc tgagtatcgc cccaacggtt
    12481 tgccggaata tgatcaagaa cgcgctgatt ggtttctgga ttactgcgtg tcgccagcgt
    12541 aatatctgtt gcataatagc caggggcaat accatttacc tgaatattat attgacctag
    12601 ttcatcacaa taagctttgg tgaatccggc aagagcatgt ttagtggcag aatatgcagg
    12661 tgaccattgt ccacctaagt aagagaacaa tgaacagata ttaataattt tgccgctttt
    12721 ctgcgggatc ataatttttg ccgcttcata gcttaactcg aatgcggcgg tcaggttcac
    12781 atcaatcatc ggatcccagt cggcacgacc gaagtccagc accttattca gcttacaaat
    12841 accggcattg tttaccagaa tatcaactgt accgaaacgc tcacagcaag cagcgataat
    12901 cttctgcggc gcgccttctg cggtgatatc cacctgcatg aagtccacct caacaccctg
    12961 tttttcaatc atttcctttg tttcgccgtt atctttgacg aaactaggaa taaagatatt
    13021 tgcgccagct ttggccaacg ccatggcaaa tgcctggcct aaaccgctat tcccaccggt
    13081 aacaattgcg gttttacctt tcagtgagaa aaaatccatt gagaacgcat tgagagattc
    13141 gattgacata gttagctcca atttccgtaa ggcaaaaaaa aagaaaggta acttcccccg
    13201 cgagagggaa gttacctttc tcattatctc tggtaactgg agataatttt tagcataacg
    13261 gcataactgc taatgtgatc gtaatcacag tgtgatattc gtattgtcaa atagttttaa
    13321 aaagcagaag cgtgattact ctcacacaac acctctgctt tccatgttat ttcttattgc
    13381 aattactaga gacctgagaa aagtaatcca cctgaaaggg cggcttactt ttctcttttt
    13441 tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg ttggataacc
    13501 ctcgcgataa ttagttttag cggcggcgtt agttttgacc tggcttattt acgttatatt
    13561 tatcaaattc ccatggcgaa atttatggga ttcagcaata ccgagatagg tttaataatg
    13621 agtacctttg gtattgcggc tattattctt tatgccccca gcggcgttat tgccgataaa
    13681 ttttcacacc gcaaaatgat tacttccgcg atgatcatta ccggattact gggtctgtta
    13741 atggcaacgt atccaccgct gtgggtaatg ctctgtattc aggtcgcctt tgcgataacg
    13801 acgattttaa tgctgtggtc ggtgtcgatt aaagccgcct cgttgctggg cgatcatagt
    13861 gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg
    13921 gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc cagcctgaaa
    13981 acagtcatta tcatctacag tgtggtttac atcttgttgg ggattctgtg ctggtttttt
    14041 gttagcgata acaacaacct gcgcagtgcc aataacgaag aaaaacagtc attccagctt
    14101 agcgacatcc ttgccgtttt gcgtatcagc accacctggt attgcagcat ggtgattttt
    14161 ggtatcttca caatctacgc cattctgagt tactccacca actatctgac cgaaatgtac
    14221 ggcatgtcgc tggtggcggc gagctacatg gggattgtga tcaacaaaat cttccgcgcg
    14281 ctgtgcggcc cacttggcgg cattatcacc acctacagta aagtgaaatc ccctacccgc
    14341 gtgatccaaa tcctttccgt actcggcctg ctgacgttaa ctgccctgct cgtcacgaac
    14401 tctaacccgc aatcggtcgc gatggggatt ggcctgattt tactgctggg attcacctgt
    14461 tacgcctcac gcgggctgta ctgggcctgc cctggcgaag cgagaacacc gtcttacatt
    14521 atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt cttcgtttac
    14581 ccaattatcg gccactggca agacaccctg cccgctgcag aagcctaccg caatatgtgg
    14641 ctgatgggca tggcggcgct tggcatggtg attgtcttta cctttttgct gttccaaaaa
    14701 attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccaa gaaaattaca
    14761 ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt cagagcacaa
    14821 aagtggtgat gtacgatctg gaaggtaacg tggtttgcga aggcaaaggc ttattacagc
    14881 cgatgcacac gccagatgcc gatactgcag aacatcctga cgacgattta tgggcatcat
    14941 tatgttttgc cggtcacgat ttgatgagtc agtttgccgg gaataaagaa gatattgtcg
    15001 gtattggtct gggatccatc cgttgctgtc gtgcgttatt gaaagccgat ggcacgcctg
    15061 ctgcgccgtt gattagctgg caggatgcac gcgttacacg cccttatgaa cacactaatc
    15121 ctgacgtggc atatgtcacc tctttttcgg gttatctgac gcatcgctta accggcgagt
    15181 ttaaagacaa tatcgccaac tattttggtc agtggccggt ggattataag agctggacat
    15241 ggagcgaaga tgctgcggta atggataagt ttaatatccc ccgtcatatg ctgtttgatg
    15301 tgcaaatgcc tggcaccgtc ctcggacata tcacaccaca agccgcactg gcgacacatt
    15361 tcccggcagg actgccggtt gtttgtacca ccagtgataa accggtagaa gctctggggg
    15421 ccggattact ggatgatgaa actgcggtaa tttctcttgg cacttacatc gcattgatga
    15481 tgaacggcaa agcactgccg aaagatccgg tggcgtactg gccgattatg tcttctattc
    15541 cgcaaacatt gctgtatgaa ggttacggta ttcgcaaagg tatgtggacg gtgagctggc
    15601 tgcgtgacat gttaggcgag tcgttaattc aggatgccag ggcgcaggat ctttcaccgg
    15661 aagatttact caacaagaaa gcctctagtg tgccacctgg ctgtaatggg ctgatgacgg
    15721 tgctggactg gctgaccaat ccgtgggaac cgtacaaacg cgggattatg atcggctttg
    15781 attccagcat ggattacgca tggatatatc gttcgatatt ggaaagcgtg gcgctaacgc
    15841 tgaagaacaa ttacgacaat atgtgtaatg aaatgaatca ctttgcgaag catgtgatca
    15901 ttactggcgg cggttcgaac agcgatctgt ttatgcaaat ttttgccgac gtgttcaacc
    15961 ttccggcacg ccgtaacgcc attaacggtt gtgcaagtct gggagcagcg attaatacag
    16021 cggtaggtct ggggctatac ccggattacg caacggctgt tgataagatg gttcgcgtga
    16081 aagatatctt tataccgatt gagagcaatg ccaaacgcta cgacgcgatg aataaaggca
    16141 ttttcaaaga cctaaccaaa catactgatg tgatcctgaa aaaatcgtat gaagtgatgc
    16201 atggggaatt ggggaatgtg gattcgatcc agagctggtc gaatgcgtaa gtgaggaagg
    16261 ccgggcggga aactgcccgg cctgaacata cctgaatggt tatccccgct gacgcgggga
    16321 acataagttc gacaacttaa aaaatgataa caagtccggt ttatccccgc tgatgcaggg
    16381 aacataataa aaactttaac tagttcggtt tatccctgtt tttacaagga atattaacta
    16441 cactcgatgt ctttagaata aaagacaata cgaatcgcac tttatcaatt ttcacttaaa
    16501 gtatgaactg gatatcttta t
//
LOCUS       DAZFAH010000100        16268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFAH010000100 DAZFAH010000000
VERSION     DAZFAH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.100"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..137)
                     /locus_tag="ACHJWB_002958"
     CDS             complement(<1..137)
                     /locus_tag="ACHJWB_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314551.1"
                     /translation="MEAVFDALKAMGKATSVELAARLDISREEAVNELWELKRNGVVD
                     KT"
     gene            complement(159..905)
                     /gene="ydaV"
                     /locus_tag="ACHJWB_002959"
     CDS             complement(159..905)
                     /gene="ydaV"
                     /locus_tag="ACHJWB_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4314552.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFSFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIAK"
     gene            complement(912..1700)
                     /locus_tag="ACHJWB_002960"
     CDS             complement(912..1700)
                     /locus_tag="ACHJWB_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314553.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAGSVTQTDQKALPEEEEEEDINTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(1778..2200)
                     /gene="ydaT"
                     /locus_tag="ACHJWB_002961"
     CDS             complement(1778..2200)
                     /gene="ydaT"
                     /locus_tag="ACHJWB_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4314554.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(2197..2439)
                     /locus_tag="ACHJWB_002962"
     CDS             complement(2197..2439)
                     /locus_tag="ACHJWB_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4314555.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2536..2955
                     /locus_tag="ACHJWB_002963"
     CDS             2536..2955
                     /locus_tag="ACHJWB_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4314556.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRLKDNPPPE"
     gene            3262..3414
                     /locus_tag="ACHJWB_002964"
     CDS             3262..3414
                     /locus_tag="ACHJWB_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4314557.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKSFKTEAGARRW
                     LAKNTS"
     gene            3823..4044
                     /gene="kilR"
                     /locus_tag="ACHJWB_002965"
     CDS             3823..4044
                     /gene="kilR"
                     /locus_tag="ACHJWB_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO4314558.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            4044..4214
                     /locus_tag="ACHJWB_002966"
     CDS             4044..4214
                     /locus_tag="ACHJWB_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO4314559.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            4289..4564
                     /locus_tag="ACHJWB_002967"
     CDS             4289..4564
                     /locus_tag="ACHJWB_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314560.1"
                     /translation="MITSYEATVVTTDDIVHEVTLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            4666..7338
                     /gene="recE"
                     /locus_tag="ACHJWB_002968"
     CDS             4666..7338
                     /gene="recE"
                     /locus_tag="ACHJWB_002968"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO4314561.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHHD
                     NAQETKELARGQEENAQADAHGDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQREHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEEKQDDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKY
                     QKLRAELHEARKNIPPKNPVDADKLLAAYRGEFVEGISDPNDPKWVKGIQTRDSVYQH
                     QPKTEQNDRKSEQNGQNARQNEPEAQQEPEKVCAACGQTDGDNCPDCGAVMGDATYQE
                     TFDEENQIEAKENDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIAGDIEPGIY
                     YGISNENYHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEP
                     EEFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQSVMALP
                     LGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYY
                     DYRYHVQDAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPIEIFMMGEEAKLAGQQEY
                     HRNLRTLADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            7331..8140
                     /gene="recT"
                     /locus_tag="ACHJWB_002969"
     CDS             7331..8140
                     /gene="recT"
                     /locus_tag="ACHJWB_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO4314562.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            8197..8391
                     /gene="ralR"
                     /locus_tag="ACHJWB_002970"
     CDS             8197..8391
                     /gene="ralR"
                     /locus_tag="ACHJWB_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO4314563.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKDISGYYRAKCNGCESRTGYGGSEK
                     EALERWDKRTTENINGGVHV"
     gene            8384..8572
                     /locus_tag="ACHJWB_002971"
     CDS             8384..8572
                     /locus_tag="ACHJWB_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4314564.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            8672..8887
                     /gene="xisR"
                     /locus_tag="ACHJWB_002972"
     CDS             8672..8887
                     /gene="xisR"
                     /locus_tag="ACHJWB_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO4314565.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            8889..10124
                     /gene="intR"
                     /locus_tag="ACHJWB_002973"
     CDS             8889..10124
                     /gene="intR"
                     /locus_tag="ACHJWB_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4314566.1"
                     /translation="MSKLPTGVEIRGRNIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISYIRYSDILNYRNKLLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGTVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            10176..11111
                     /gene="ttcA"
                     /locus_tag="ACHJWB_002974"
     CDS             10176..11111
                     /gene="ttcA"
                     /locus_tag="ACHJWB_002974"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4314567.1"
                     /translation="MQENQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(11240..12613)
                     /gene="dbpA"
                     /locus_tag="ACHJWB_002975"
     CDS             complement(11240..12613)
                     /gene="dbpA"
                     /locus_tag="ACHJWB_002975"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4314568.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALALHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQITLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVTVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(12643..12816)
                     /gene="ynaL"
                     /locus_tag="ACHJWB_002976"
     CDS             complement(12643..12816)
                     /gene="ynaL"
                     /locus_tag="ACHJWB_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4314569.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(13091..14074)
                     /gene="zntB"
                     /locus_tag="ACHJWB_002977"
     CDS             complement(13091..14074)
                     /gene="zntB"
                     /locus_tag="ACHJWB_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4314570.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(14088..14135)
                     /gene="ydaN"
                     /locus_tag="ACHJWB_002978"
     CDS             complement(14088..14135)
                     /gene="ydaN"
                     /locus_tag="ACHJWB_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4314571.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            14329..15561
                     /gene="dgcM"
                     /locus_tag="ACHJWB_002979"
     CDS             14329..15561
                     /gene="dgcM"
                     /locus_tag="ACHJWB_002979"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4314572.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARPLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(15582..16145)
                     /gene="smrA"
                     /locus_tag="ACHJWB_002980"
     CDS             complement(15582..16145)
                     /gene="smrA"
                     /locus_tag="ACHJWB_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4314573.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         4563 a         3951 c         3836 g         3918 t
ORIGIN      
        1 gttttatcaa cgacgccatt tcttttgagt tcccacagct cgttgacagc ctcttcacga
       61 ctgatatcaa gtcgcgcagc cagttctacc gacgtggctt ttcccattgc tttcagtgcg
      121 tcaaatacag cttccattaa aacgtcctcc aacaaaaatt acttcgcaat tcctggctga
      181 ccaacattcg gacgccagct ctcccagtta aaattcaccc atcgcccgcc gttcatggtc
      241 atgcgatcca taatccgctc accgagcaat gttttcatgg cctcatagtt caggtttgtc
      301 agcatcccca cgctgcgcat cgacgctgtc cggcgatcaa caatctgatg cagcaccacc
      361 tgctcgtttt tcgtctcgcg ctgaatgcca atttcatcaa gaaccagcag atccacttcg
      421 cacagctccc gcaaaaattt ttcgcctgac tgcccgtcgt catagctggc gtgcagggcg
      481 ctcataacat cagccacggt aaccacaatc actgtctgac cgtctttcag caggcgattc
      541 ccgatagctg ccgctaagtg attcttcccg gtaccaggtt ttccgctgaa cgaaaaattt
      601 gtacacccgg tcatcagttc atcggcgatg gatttcgcct ggcttaacgc gtatcgctgg
      661 ccgtcgttct gcacctggta attcgcaaac gagcatttac ggtgcaacgg ctggatgcca
      721 gagcgattca gaattttttc cacccgcaac tgacgattca ggcggttgat ctcctcgcaa
      781 cgtttctgtc cttcagcaag ttgccactca cgccactccg caaccgttct gaatggggcg
      841 gttacatgtg gtggggtcag tctgcggata cgctccagaa cgccgcctgt cgcaatattt
      901 ttcatggtct gttaccccct gaagcctggc gggatcgcac tatccggcaa cgagacggtg
      961 ttaacctgtc ggagcaacgt ctcaggccga acacctttcg gtgcgaacaa gccctggtat
     1021 tcattggcaa tgctgtgtcg aatcacctgc tcaggtgtaa aaccctgctg acggaatttt
     1081 tccagttccc gtatcgcccc gttagcgccc tgctccgttc gaatcggttt tcgcaatgcc
     1141 tgcctgaact gaacccactc atgccagagt gtttccggca accagtcagg cagctcgata
     1201 gcctccggct cgaatttttt agacgctcgt ttttggcgag ggggatttag ggggagatca
     1261 gtatttatat cttcctcttc ctcttcctct ggtaacgctt tttgatccgt ttgtgtaacg
     1321 ctgccagcgt taccttttcg tttcagttcg cgtatttttg taactcgctc gtttgtaacc
     1381 gcccgttttt tagagctttt tccgttatga cgttcaaagt taggtagaga aagcccaccg
     1441 tcattttcga ccagccatcc aacctgaatt aacgcatcag caaaaccagc cataaaagtg
     1501 atgcgatcta ttgcactttt tgtaacgccg cgagcgttac aatctgcatt accgtctatc
     1561 atttgttgat ccgcccatgc ccagaagcga ataaccttcc ctaatgcggc atctggatca
     1621 atattcagaa tctcagcaag cctgaatatt tccggcttat ccggcgtaat aacctcgagc
     1681 tttatccagt ttgaagccat ttgttttcac cttgtaacgc tcgcagcgtt acatttaact
     1741 gataccgaac aaaacaatcc ggcacgatta atttcaatca atgcactacg acagaatcgc
     1801 cgggcgaccc accaccgctg aaatgtgctt tccggtaaac ggcctggact gcatcatcat
     1861 gcgcatcaat tgccgtactc aacgcttcct gcgccgccag taatgcacgg cgttccaagg
     1921 tatcgaagat gcagagtcgg tgacgcagct cgcgcggaag aattgccaga accgcaggga
     1981 tcagtttctg aattttttcc ctttgcgctt tcgtttcacc tttcaaccaa cggtgataga
     2041 tattctgctg attgttccag tccttgcctg gtacaagggg caattcgccg cccccctggc
     2101 gcagatattc ttcagtaatt gcgttagcga cccacgcctg ccctttttcg gctgccaggg
     2161 ctaacaacac tgattcgatg tgctcatgcc tgattttcat gaatcaaccg ctcctatgct
     2221 gttttcgcta tgcttaccgt ctggggggaa tacatcgtca agtccacaat gagcgccaag
     2281 ccgattaagg gtagaaacaa tttttctgca ctcctctagt ccaggggtac gaaaatttgc
     2341 ttcgtaattt gccagtcggc tttgtatcca ccctaactga acagcaagtt gtctttgaga
     2401 cagcccaagc tgttttcgat atgttgaaat tttgttcatt gaaaacctcc gatgacaatt
     2461 ttaaacacat cttgtgttat atggtcaagc tgttttgtgt tttgtgtaaa tcacgattcg
     2521 tgatacaagg atgcaatgga aaaagaaaac gaaaaaattg ccgctagtag gctcaatgac
     2581 aaaattgcaa tgcgtcttaa agagcgcagg cagaagcttg gtttatctca aggaaaactt
     2641 gctgaaatct gcggatggac gcaatcgcgt ataggtaact atgaggcggg tagcagaaat
     2701 gttggagtgc atgacgctgt cgtattggga aaggcacttg gcatatctcc tcctgagctc
     2761 ctctttggag aacaggaatc ttctgaattg tggttaaatg aatcccaacg aaaacttctt
     2821 gagttgttta accagctacc gggctcagaa caacaacgaa tgattgagct atttgaagtc
     2881 cggctaaaag aaatcgatga gtatgtagaa aaatatttga gaggcaggct taaagataat
     2941 cccccaccgg agtaatgatc ttgctatcac agtaatatgc caatcagccc gctatcagcg
     3001 ggcttttttt gtaccatcat catatgacac ccaccacaaa acacatttcg tgttgacaca
     3061 agaaaacgaa ttgtgtttaa taagcatatc caaacaacgc cccaccagag aacggcagga
     3121 caatacctcg agttatccag ccactgaaca gggctaagta gccagcctga ggcatacgaa
     3181 catgacggca gttgttgatt gatacaaagc gcagtagata aaacgttccg ccacccggcg
     3241 ttaaggggaa atgaggtcaa catggatact atcgatcttg gcaacaacga atctctggta
     3301 tgtggcgtgt ttcccaatca ggacggcaca ttcaccgcca tgacgtatac caaaagcaaa
     3361 tcgtttaaaa ccgaagctgg cgcacgtcgc tggcttgcca aaaataccag ctaaaccatt
     3421 tattggatta attcaatatt cttgctgtag gggtatagcc gaggccacca aagcccggag
     3481 gtggtgaaat aaaaccgggc acaacacgaa ggcgcatttc cggtatccat aaagagtcgg
     3541 tcttgtctgt taaatttaaa tggtgggagt gcgcctccgg ttgtaaataa cgacattgct
     3601 atgtgtagtc tttggcggca tcagttctac tccgtggctg ccctgccgcc cctttttaaa
     3661 gtgaattttg tgatgcggtg aatgcggcta agcgcacgcg gcacagttaa aagcatcagt
     3721 gttatgggtg gattatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag
     3781 gcaccgcatc gacaaaattc atttgtaaaa atggagataa ttatgattgc tcatcacttc
     3841 ggaactgatg aaataccacg tcagtgtgtg actcctggcg attatgttct tcatgaaggt
     3901 cggacatata tcgcctcggc aaacaatatt aaaaagcgaa aactttatat tcgtagcctg
     3961 actacaaaaa catgcatttc tgactgcatg attaaagtct tcctcggtcg tgatggttta
     4021 cctgtaaagg cggagtcatg gtaatgacta agaaaataaa atgtgcttat cacctttgca
     4081 ataaagaaat tgaagaaagc aaaatcatta caagaccact tcatttcatg cgtggagtta
     4141 taccaacgac ggaaatgaaa aaatattgta gtgaaatctg tgccgaaaaa gaccagatgg
     4201 cacacgaact ttaattaact gactatccga aactgaattt atgccagcaa tggcagggat
     4261 tcgctcaacc ttaattaagg agaaaaacat gattaccagt tatgaagcca ctgttgttac
     4321 tactgatgat attgttcacg aagttaccct ggaaggaaag cgtattggct acgtgattaa
     4381 gacagaaaat aaagaaaccc cattcactgt ggttgatatc gacggtccat caggcaacgt
     4441 taaaacactt aacgatggtg ttaaaaaaat gtgtctggtg cacataggaa agaatctgcc
     4501 cgcagaaaaa aaagccgaat ttctggcaac tctgattgca atgaaattaa aaggtgaaat
     4561 ctgaaagaaa tagcctgcgt atggcgcagg ctatgaacag tgtgtatccg gcaagatcat
     4621 tcactgaaca aaacgaattt taatctgagt tgaggttaaa aaacaatgag cacaaaacca
     4681 ctcttcctgt tacggaaagc gaaaaaatca tccggtgaac ctgacgtcgt cctgtgggca
     4741 agcgacgatt ttgaatcgac cagtgccact ctggactacc tgatcgttaa gtcaggtaaa
     4801 aaactgagca gttattttaa agctgttgcc acgaattttc ctgtcgttaa tgaccttccc
     4861 cctgaaggtg agatcgattt tacctggagt gaacgctatc aactcagcaa agactccatg
     4921 acctgggaac taaaaccggg agcagcgcca gacgacgttc accaccatga taatgctcag
     4981 gaaaccaaag aactggcgag gggccaggaa gaaaacgcgc aggcagacgc ccacggggat
     5041 tgccaggatt gcgaagtctc tgtagccact ttgcggttca cacagcgtct tctgcacatt
     5101 tttacgtatg cggccgggga tcggaaatac ctgcatcatg ccacccgtga acaacgcgaa
     5161 cacatcactg ctcttgagat ggatcaggaa aacagctatg tccagaatct gctgttggcc
     5221 atacgcggca tggcagaacc gacaactctg gataatgccg ccctgctccg cctgactgat
     5281 gcaattaagg cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc
     5341 atttcagcct ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga
     5401 aaagggaatc gtgtttcacg catcactcgc acggcttccg gtgctaatgc tggcggcggg
     5461 aacctcaccg atcgcggcga aggtttcgtc cacgatctga cgtcactggc gcgcgatgta
     5521 gccactggcg tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac
     5581 gctaaacgca ttgaggaaat tatcgctgaa aataaaccgc ccttttctgt tttccgcgac
     5641 aaattcatca ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg
     5701 aaagaagcac caattgggat cgaggtcatc cccgcgcacg tcactgaata tctgaacaaa
     5761 gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt
     5821 cgctcctctg ccccgatgcc gcagcgtgta acagaagaag aaaaacagga tgatgaagaa
     5881 aaaccgcaac catcttgcgc aatggcagat gaacaggcaa cggctgaaac agtggaaccg
     5941 gatgcaactg aacatcatca ggacacgcag ccgctggatg ctcagtcaca ggtaaattct
     6001 gttgatgcga aatatcagaa actgcgggca gaactccatg aagcccggaa aaacattccg
     6061 cccaaaaatc ctgtcgatgc agacaaatta ctggctgcct atcgcggaga atttgttgaa
     6121 gggattagcg acccgaatga tccgaaatgg gttaagggga tccagacccg cgattctgtg
     6181 taccagcatc agccaaaaac ggaacagaac gaccgaaaat cggaacaaaa cggccaaaat
     6241 gcgcgacaaa atgaaccaga agcgcaacag gaaccggaaa aagtctgcgc cgcctgcggt
     6301 cagactgacg gggataactg ccctgactgt ggtgcggtaa tgggcgacgc aacataccag
     6361 gaaacattcg atgaagagaa tcagattgaa gctaaggaaa atgatccgga ggaaatggaa
     6421 ggcgctgaac atccgcacaa tgagaatgct ggcagcgatc cgcatcgcga ttgcagtgat
     6481 gaaactggcg aagtcgcaga ccccgtaatc gcaggagaca tagagcctgg tatttattac
     6541 ggaatttcga atgagaatta ccacgcgggt cccggtgtca gtaagtctca gctcgacgac
     6601 attgctgata ctccggcgct gtatttgtgg cgtaaaaatg cccctgtgga caccacaaag
     6661 acaaaaacgc tcgatttagg aaccgctttc cactgccgtg tacttgaacc ggaagaattc
     6721 agtaaccgct ttatcgtagc acctgaattt aaccgccgta caaacgccgg aaaagaagaa
     6781 gagaaagcgt ttctgatgga atgcgcaagc acaggaaaaa cggttatcac tgccgaagaa
     6841 ggccggaaaa ttgaactcat gtatcagagc gttatggctt tgccgctggg tcaatggctt
     6901 gtggaaagcg ccggacacgc tgaatcatca atttactggg aagatccgga aacaggaatt
     6961 ttgtgtcggt gccgtccgga caaaattatt cctgaatttc actggatcat ggacgtgaaa
     7021 accacagcgg atattcaacg attcaaaacg gcttattacg actaccgcta tcacgttcag
     7081 gatgcattct acagtgacgg ttatgaagca cagtttggtg tgctgccaac tttcgttttt
     7141 ctggttgcca gcacaactgt tgaatgcgga cgttacccga ttgagatttt catgatgggc
     7201 gaagaagcaa aactggcagg ccagcaggaa tatcaccgca atctgaggac cctggctgac
     7261 tgcctcaata ccgatgaatg gccagctatt aagacgttat cactgccccg ctgggctaag
     7321 gaatatgcaa atgactaagc aaccaccaat cgcaaaagcc gatctgcaaa aaactcaggg
     7381 aaaccgtgca ccagcagcag ttaaaaataa cgacgtgatt agttttatta accagccatc
     7441 aatgaaagag caactggcag cagctcttcc acgccatatg acggctgaac gtatgatccg
     7501 tatcgccacc acagaaattc gtaaagttcc agcgttagga aactgtgaca ctatgagttt
     7561 tgtcagtgca atcgtacagt gttcacagct cggccttgag ccaggtagcg cccttggcca
     7621 cgcatattta ctgccttttg gtaataaaaa cgaaaagagc ggtaaaaaga acgttcagct
     7681 aatcattggc tatcgcggca tgattgatct ggctcgccgt tctggtcaaa tcgcaagcct
     7741 gtcagcccgt gttgtccgtg aaggtgacga gtttagcttc gaatttggcc ttgatgaaaa
     7801 gttaatacac cgcccgggag aaaacgaaga tgcaccagtg acccacgtct atgctgtcgc
     7861 aagactgaaa gacggaggga ctcagtttga agttatgacg cgcaaacaga ttgaactggt
     7921 gcgcagccag agtaaggcag gtaataacgg gccgtgggta actcactggg aagaaatggc
     7981 aaaaaaaacg gctattcgtc gcctgtttaa atacctgcct gtctcaattg aaatccagcg
     8041 tgcagtatca atggatgaaa aggaaccact gacaatcgat ccggcagact cctctgtatt
     8101 aaccggggaa tacagtgtaa tcgataattc agaagaataa ttcagccagg cggtgtaatg
     8161 caccgccaac gtgagatagt ttttatgaca aaaactttga gatatgacga tgttaaacca
     8221 tgtccgtttt gtggttgtcc atcagtaacg gtgaaagaca tttcaggata ttaccgggca
     8281 aaatgcaacg gatgcgaatc ccgaactggc tatggtggaa gtgaaaaaga agcgctcgaa
     8341 agatgggata aacgaaccac tgaaaatatt aatggaggcg ttcatgtata aaattaccgc
     8401 tacaattgaa aaggaaggtg gcactcctac taactggaca agatattcaa aatctaaact
     8461 aacgaaatca gaatgcgaaa aaatgctctc aggtaaaaaa gaagcaggcg tttccagaga
     8521 gcagaaagta aaactgataa attttaattg cgagaaactt cagtcctcgt gaattgcatt
     8581 gtattcaaat taaaacttca tagctgatta ttaataatca acatcaggcg tcaatttcag
     8641 tctaacattg gcgcctgcca gaggtgatgc gatggcacaa gtaatcttta atgaagagtg
     8701 gatggttgaa tacggcctga tgcttcgcac tggtctgggg gccagacaaa ttgaagcata
     8761 ccgccagaac tgttgggtgg agggcttcca cttcaaacga gtatctcctt taggtaagcc
     8821 agacagcaaa cgagggatta tctggtacaa ctatccaaag ataaatcagt ttatcaaaga
     8881 ctcatgatat gtctaaatta ccaacaggtg tcgagattag aggtagaaac attcgcatct
     8941 ggttcatgtt tcgaggaaaa cgatgtcggg aaacattaaa aggctgggag attacaaaca
     9001 gtaatattaa aaaggccgga aatttaagag cgctgatagt tcatgaaata aactccggtg
     9061 aatttgagta tttaagacgt tttccccagt ccagcactgg ggcaaaaatg gtgacaacga
     9121 gagtcataaa aacgttcggg gagctttgtg atatctggac aaaaattaaa gagacagagt
     9181 taacaacaaa cacaatgaag aaaacgaaat cacaattaaa aacactcaga ataataattt
     9241 gtgaaagtac cccgatatca tatattcgtt atagcgatat cttaaactac cggaataaac
     9301 tgctgcatgg agaaacgctt tacctggata atccaagatc caacaaaaaa ggaagaaccg
     9361 tgcgcacagt tgataactat atcgccctgc tctgttcgct gttacgtttt gcgtatcagt
     9421 cgggatttat atcaaccaaa ccatttgaag gagtaaaaaa attacagcga aacagaataa
     9481 agcctgaccc gttatctaaa acagaattca atgcattaat ggaaagtgaa aaaggacaga
     9541 gccaaaactt gtggaaattt gccgtttact ccgggcttcg tcacggggaa ctggcagctc
     9601 tggcgtggga ggatgtggat ttcgagaagg gaactgtgaa tgtcagaaga aacctgacga
     9661 tactggatat gttcggtccc ccaaaaacaa atgccgggat ccggacagta acattactgc
     9721 agcctgctct tgaagcactg aaggagcaat acaaactgac cgggcatcat cgcaaaagcg
     9781 aaatcacctt ttatcatcgg gagtacggca gaaccgaaaa gcaaaaactg cattttgttt
     9841 tcatgcccag ggtgtgtaac ggaaaacaga aaccttatta ctcggtaagc agtttggggg
     9901 caaggtggaa tgcagcagta aaacgtgctg gtattcgccg ccgtaatccg taccatacgc
     9961 ggcatacttt tgcctgctgg ctgttgacgg caggagcgaa cccggcattt atagccagcc
    10021 agatggggca tgaaactgcg cagatggtgt atgaaattta cggtatgtgg attgatgata
    10081 tgaacgacga acaggtagcc atgttgaatg cgcggctatc gtagttgcaa agtttgcccc
    10141 caatttgccc catttagtac cagagaaccg aaataatgca agaaaatcaa aagaatacga
    10201 agaaagaaca atacaacctg aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag
    10261 ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc ctctccgggg
    10321 gtaaagacag ctataccatg ctggagattc tgcgcaattt gcagcaaagc gcgccaatca
    10381 atttttcgct ggtggctgtt aacctcgatc aaaagcaacc aggcttcccg gaacacgttc
    10441 tgcccgagta tcttgaaaag ctgggcgttg agtacaagat tgtcgaagag aacacttacg
    10501 gtatcgtgaa agagaagatt ccagagggca aaaccacttg ctcactgtgt tctcgccttc
    10561 gtcgcggtat cctttatcgt accgcaacgg aactgggggc gacgaagatc gcgctgggtc
    10621 accatcgtga cgatatcctg cagacgctgt tcttaaatat gttctacggc ggcaagatga
    10681 aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tatcgttatt cgtccgctgg
    10741 cctactgccg cgagaaagat attcagcgct ttgccgatgc aaaagcgttc ccgattattc
    10801 cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct gacatgttgc
    10861 gtgactggga taaacgttat ccagggcgta tcgagacgat gttcagcgcg atgcagaatg
    10921 tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggcatc acccacggtt
    10981 ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccca ctacaaccgg
    11041 cgggctggca gccagaagaa gatgaaaatc agttggatga gttacggctg aatgtggttg
    11101 aagtgaaata accaggatag cgcccgatgc gcaagcgtat cgggctactc ttatggaggc
    11161 cggataagac gcggccagcg tcgcatccgg caatcccgaa taagatgttt actcttgcac
    11221 ccggcaattc aacatttcat tattttaata accgcacccg gcatgttttt cctttaatct
    11281 tcccgccctg taactgtttc catgctttat gagcaacagc ctgacggacc gtgacataga
    11341 catgcgccgg atgcacggcg attttgccaa tatctgcgcc atcaagcccg atatctcctg
    11401 tcagtgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca tcgatacaca
    11461 acgttgccat ttctgcttcc agcggcacaa tggaactatt aactggcggt gtttgccagt
    11521 taagtgttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct tccggggcac
    11581 agaaactgat cgccagaccg ctatttcctg cacgagctgt acgaccgatg cgatgtacat
    11641 gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat ttaatatcca
    11701 gaccacgcgc agcaacatca gtcgcaacca gtacacgggc gctaccgtta gcaaaacgta
    11761 ccagagtctg atcgcgatcg cgttgctcca gatcgccgtg taacgccaat gcactttgcc
    11821 ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca
    11881 cgcaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttgc
    11941 tggatgtctc ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa
    12001 tcgccaaagg atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg
    12061 ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat
    12121 cgctaaatcc catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat
    12181 ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa
    12241 tatgcggcgc atgttgcaac gaatcacgct gcataccaaa cggttgacca ccgcacaacg
    12301 tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcaccg gccacctgat
    12361 ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc gacgcatcaa
    12421 tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca
    12481 cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg
    12541 ttaaataacc caactcatta aggttcgtga gttgggcagg aggcaaaaca ttcagggtag
    12601 aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct
    12661 cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg
    12721 catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc
    12781 agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga
    12841 ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg
    12901 cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag
    12961 cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt
    13021 agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccggaaaa
    13081 attgcttttg ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa
    13141 caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac
    13201 caaggttgac gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca
    13261 acgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg
    13321 ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat
    13381 cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg
    13441 caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt
    13501 tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa
    13561 ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg gtcaacgcat
    13621 cgcacacatc caccagccac cctccgcaat cggtcggacc cgtgccctct tccagatcgc
    13681 tcaccacatc gtccagcgcc aacactttgc gttgtcgggt cgaaacaatt aaccgcccgt
    13741 ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac
    13801 agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccgt gtgctctcac
    13861 ctgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcac
    13921 tgtcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg
    13981 tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg
    14041 ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt
    14101 ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg
    14161 attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc
    14221 gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt
    14281 ttttgcgatc ggatagcaac aaaaattgat aaaaataatg ggatctcaat gattacgcac
    14341 aacttcaata ccctggactt actcaccagt cctgtctgga tcgtttcgcc ctttgaggaa
    14401 cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag
    14461 ctacgaaccg gtccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat
    14521 ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca
    14581 gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc
    14641 gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag
    14701 cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg
    14761 attgacccgt cacgagatgg acaaatcgtc gatgcaaacc tcgccgcgct caatttctat
    14821 ggttataacc atgaaacgat gtgccagaaa catacctggg aaataaatat gctcgggcgt
    14881 cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa acctttgaat
    14941 tttgttcata aactggcgga tggttctact cgtcatgtgc agacctatgc cggaccgatt
    15001 gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg
    15061 ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg
    15121 cgacagtttt atcacattac ggaaccaggc caaatgcagc atctcgccat cgctcaggat
    15181 tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat
    15241 tctaaaggtg atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg cgctcgcaaa
    15301 ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca
    15361 ctggataccg cgctttcgct ggctgaaact atccgtgtaa gcgtggcaaa agtgagtatt
    15421 tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc
    15481 atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc
    15541 aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacggct gcgcttagcg
    15601 tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc
    15661 ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc
    15721 agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc ccgtcctttg
    15781 ccatgaataa tcagcacgtt acgcaaacca tcagccatgg cttgttgaat gaaactgaac
    15841 atcattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga
    15901 tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg ccgaaactcc
    15961 agcggctgac ttagtgggat gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc
    16021 agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatcggta
    16081 gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg
    16141 ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg ggtcggtcca
    16201 acaacgtatt acccaaaatt ccagtaataa gttccaaata ttgccgatat tttaagcaaa
    16261 atgcttat
//
LOCUS       DAZFAH010000101        15655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFAH010000101 DAZFAH010000000
VERSION     DAZFAH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.101"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..1478
                     /locus_tag="ACHJWB_002981"
     CDS             114..1478
                     /locus_tag="ACHJWB_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4314574.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHJWB_002982"
     CDS             complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHJWB_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4314575.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2265..3176
                     /gene="panE"
                     /locus_tag="ACHJWB_002983"
     CDS             2265..3176
                     /gene="panE"
                     /locus_tag="ACHJWB_002983"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4314576.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3139..3729
                     /gene="yajL"
                     /locus_tag="ACHJWB_002984"
     CDS             3139..3729
                     /gene="yajL"
                     /locus_tag="ACHJWB_002984"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4314577.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHJWB_002985"
     CDS             complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHJWB_002985"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4314578.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVVHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5437..5679
                     /gene="xseB"
                     /locus_tag="ACHJWB_002986"
     CDS             5437..5679
                     /gene="xseB"
                     /locus_tag="ACHJWB_002986"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4314579.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5679..6578
                     /gene="ispA"
                     /locus_tag="ACHJWB_002987"
     CDS             5679..6578
                     /gene="ispA"
                     /locus_tag="ACHJWB_002987"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4314580.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6603..8465
                     /gene="dxs"
                     /locus_tag="ACHJWB_002988"
     CDS             6603..8465
                     /gene="dxs"
                     /locus_tag="ACHJWB_002988"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4314581.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKAIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8520..9494
                     /gene="yajO"
                     /locus_tag="ACHJWB_002989"
     CDS             8520..9494
                     /gene="yajO"
                     /locus_tag="ACHJWB_002989"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4314582.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSLLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="ACHJWB_002990"
     CDS             complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="ACHJWB_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4314583.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="ACHJWB_002991"
     CDS             complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="ACHJWB_002991"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4314584.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="ACHJWB_002992"
     CDS             complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="ACHJWB_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4314585.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="ACHJWB_002993"
     CDS             complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="ACHJWB_002993"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4314586.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="ACHJWB_002994"
     CDS             complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="ACHJWB_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4314587.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKDATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="ACHJWB_002995"
     CDS             complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="ACHJWB_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4314588.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13804..14343
                     /locus_tag="ACHJWB_002996"
     CDS             13804..14343
                     /locus_tag="ACHJWB_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4314589.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVQQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14642..15526
                     /gene="tsx"
                     /locus_tag="ACHJWB_002997"
     CDS             14642..15526
                     /gene="tsx"
                     /locus_tag="ACHJWB_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4314590.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
BASE COUNT         3776 a         4151 c         4061 g         3667 t
ORIGIN      
        1 aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa cactctgttt
       61 acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg gtaatgaacg
      121 attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc gtattctcgt
      181 tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc atggctctgc
      241 aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg actcaggccg
      301 tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca ttaattgtcg
      361 gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac tccatctggg
      421 gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc gttatggcgc
      481 tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt atcggcgtga
      541 gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact cacaaacttg
      601 ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt gcgttgacca
      661 tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga atggtgaaag
      721 gcagtttcag taaagtgctg gcggaaccgc ggctgctgaa actcaacttt ggcattatgt
      781 gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta gctgatgcgg
      841 ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc gcctttggct
      901 cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct
      961 tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa acgcagttct
     1021 ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg gaagcccttc
     1081 tgccttcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg atgggtgttt
     1141 actccaccag ccagtttctt ggcgtggcga ttggcggttc actgggcggc tggattgacg
     1201 gcatgtttga cggtcagggc gtatttctcg ctggcgcaat gctggccgca gtgtggctgg
     1261 cagtcgccag taccatgaaa gaaccggcgt atgtcagcag tttgcgcatt gaaatcccgg
     1321 cgaacattgc cgcaaacgag gcgttaaaag tgcgtttgct agaaactgaa ggcatcaaag
     1381 aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc aaagtgacga
     1441 atcgctttga tgttgaacag gcaattcgcc aggcataaaa gcgaggcgaa attgcctgct
     1501 gcgctacgct catcaggcct actaagctcc tgatttacaa acctttgtag gccgaaccag
     1561 gcgttcatgc cgcatctgac atgaacaaag cgcaggttat cgcgattaat cgcggaagtt
     1621 tttgaactgg aacggctgac ccagatcgcc accgcgcacc atcgccatga cagcctgcaa
     1681 atcatcacga gatttgccag tcacgcggat ctcatcgccc tgaatttgcg cctgcacttt
     1741 cagtttgctg tctttgatca tcttgacgat ttttttctgg gtcgcgctct caatgccctg
     1801 cttcagtttc gcctccacaa accaagtttt accgctatga acgatatttt ccggtacatc
     1861 cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     1921 attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1981 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc tcgcgttatc
     2041 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     2101 ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat aaccgacagt
     2161 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     2221 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     2281 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag ggtcatgaag
     2341 ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt gaatctggtt gagacagatg
     2401 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc accagcgatc
     2461 tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcgtcca
     2521 cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc atcgaagagt
     2581 tgcaaaatat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     2641 gcaatgtcat tattcatgtg gcaaacggta tcacgcatat tggtccggca cggcaacagg
     2701 atggcgatta cagttatctg gcggatattt tacaaaccgt gttgcctgac gttgcctggc
     2761 ataacaatat tcgcgccgag ctgtggcgca agctggcagt caactgcgtg attaatccac
     2821 tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa gaaattatgc
     2881 agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact tcagcagaag
     2941 atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt
     3001 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     3061 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     3121 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     3181 gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa agtcaccact
     3241 gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt gaagctgctg
     3301 gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt actgcctggt
     3361 ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac cgttaaacag
     3421 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggta
     3481 ccgcacgata tcttcccgat tggcaatatg accggcttcc cgacgctgaa agacaaaatt
     3541 cccgccgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     3601 agccaggggc cgggtacggc tattgatttt ggtctgaaaa ttatcgacct gctggttggg
     3661 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     3721 tacgagtagt gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt aaaaaccaca
     3781 aattatgggc gatacacctt cacattgtta aagccctgct cgcgcagata gagcgcctgc
     3841 agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg gtcgagatcg
     3901 ccaaatttgg tgctcagttt atagaacggc agagaaacca cgtcgatacc ttcaaccttc
     3961 agcggcttat cttcctgctc atcgacagaa cggatatcga ggatcacgtc gttcgggcca
     4021 aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     4081 atatcaacgt tattcgcttc ctcaaccact ttatcgagaa tactgaagtc gaacttctct
     4141 tcttccgctt caatcttcga tttaaccgct ttcaccgtcg ggcttttgga gatcacgcca
     4201 caatattctg gcatcgtgcg agcaaagtct tcagtgccaa tctggcgggc caggttgatg
     4261 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtgtcaga gacgttatca
     4321 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4381 agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat catacgtttg
     4441 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccaac gaccggttcg
     4501 aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca cagataatgc
     4561 accacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa acagtaatgc
     4621 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     4681 atgagcgaca gcacgtcttc ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg
     4741 cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac ttccggattg
     4801 gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     4861 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     4921 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4981 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     5041 cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc ccagtggcgg
     5101 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     5161 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     5221 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     5281 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     5341 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5401 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggctccc
     5461 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5521 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     5581 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     5641 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     5701 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     5761 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc
     5821 gaccttttct ggtttatgcc accggtcata tgttcggcgt tagcacaaac acgctggacg
     5881 cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     5941 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     6001 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     6061 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     6121 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     6181 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     6241 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     6301 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6361 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6421 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6481 acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc actcgatacc tcggcactgg
     6541 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     6601 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     6661 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     6721 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     6781 ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg gatgtggggc
     6841 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     6901 agaaaggcgg tctgcatccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6961 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     7021 aaggcaaaaa tcgccgcacc gtctgtgtca ttggcgatgg cgcgattacc gctggcatgg
     7081 cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg gtcctcaacg
     7141 acaatgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa taaccatctg gcacagctgc
     7201 tttccggtaa gctttactct tcgctgcgcg aaggcgggaa aaaagttttc tctggcgttc
     7261 cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtagtgcctg
     7321 gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt cacgatgtgc
     7381 tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7441 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc actttccacg
     7501 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7561 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     7621 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     7681 cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt gccgccggtc
     7741 tggcgattgg tgggtacaaa gccattgtcg cgatttactc cactttcctg caacgcgcct
     7801 atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc gccatcgacc
     7861 gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac ctctcttacc
     7921 tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7981 tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac ccgcgcggta
     8041 acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa ggcattgtga
     8101 agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca gacgcggcga
     8161 aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccgcttgatg
     8221 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     8281 ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat cgtaaaccag
     8341 tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact caggaagaaa
     8401 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggctgg
     8461 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     8521 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     8581 tgacctttgg cgagccagat cgtggtaatc acgcatggac actgccggaa gagagtagcc
     8641 gtcccatcat taaacgcgcg ctggaaggcg gcataaattt ctttgatacc gccaatagct
     8701 attccgatgg cagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     8761 aagacgtggt cgttgcgacg aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     8821 cccgtgcaca aattttgcgc tctatcgacg acagcctgcg ccgtctcggc atggattatg
     8881 tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag acgctggaag
     8941 ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg
     9001 cttcgcagtt tgctcaggcc ctgaagctac aaaaacagca cggctgggcg cagtttatca
     9061 gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg ctgcctctgt
     9121 gttatcagga gggcgtggcg gtgattccgt ggagcctgct ggcgcggggg cgactgacgc
     9181 gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaga aatctctata
     9241 aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac
     9301 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg ggcattgccg
     9361 caccgattat cggtacatcg cgggaagaac agcttgatga gctgctgaac gcggtggata
     9421 tcactttgaa gccagaacag attgccgaac tggaaacgcc gtataaaccg catgcggtcg
     9481 taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat caggcatcgg
     9541 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatgcctg
     9601 cggaaatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca tgtacattgc
     9661 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atcacaaacc
     9721 cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc cacataccaa
     9781 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc gccgtttgat
     9841 gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag agctgccagg
     9901 gtaaaaaggt catcagatac cagaacggaa tcgctgccag cgatcccatc gtgccaggaa
     9961 cgattgggct taatccactt ccgaatccga cagcaagcag atgccacgga ttactcatct
    10021 tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc tttccagtca
    10081 aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc ggtcatttgc
    10141 ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc gccacggttc
    10201 agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
    10261 gcctgttccg gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca
    10321 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
    10381 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa aatacgcggc
    10441 gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc aacctgcaaa
    10501 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
    10561 cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
    10621 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
    10681 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
    10741 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
    10801 cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc atggaggaag
    10861 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
    10921 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    10981 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    11041 aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg cctggatatc
    11101 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    11161 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    11221 atggcacatc gctacgttta gacagttcgt acagcgcaat gcgcagtact gctttttcta
    11281 cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg tcgaggtatg
    11341 cggtattagt cgccaccccg gccagcagct cacggaagta caggacgtca acgtctttta
    11401 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    11461 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    11521 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    11581 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    11641 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    11701 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt gccaccacga
    11761 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    11821 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    11881 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    11941 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    12001 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    12061 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta aatgcaggca
    12121 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    12181 ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt taggtgcgat
    12241 atagacaatc agctcatcga ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc
    12301 cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca gtacaaccag
    12361 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcact
    12421 agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc
    12481 cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga gattttgttg
    12541 cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta aggctggatc
    12601 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg
    12661 tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    12721 gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    12781 cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt ggctgacgtc
    12841 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    12901 catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    12961 acgaccatga tggctacagg gttcgagtgt gacataggcg gtcgcatctt tggctttttc
    13021 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgcacgtt ggtggtaacc
    13081 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tgggatgcgt
    13141 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    13201 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    13261 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    13321 cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca gctgcgattt
    13381 aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt ccagcgcccg
    13441 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    13501 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    13561 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    13621 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacag
    13681 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    13741 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    13801 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    13861 tgtgtgcagc agagcgaagt gcaacagatg aaacacagcg tcagcacgct gaaccaggag
    13921 atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct gaatgcaaaa
    13981 tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact ggaaagccag
    14041 atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg caccaccctg
    14101 acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    14161 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    14221 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acagttaaag
    14281 ggtatttctg tagaacagtt agacttcgta cgtatccacg atattcaacc tgttatgcag
    14341 taaacgtatt tcggggcgat tttgtgcgtc ccgcaacatc tttccccgtc attttgttac
    14401 tccgcttaca tcacccagat tgatagtaaa agtttgcaac aagggtgaaa gtcagtacaa
    14461 tccctgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat agaactgtga
    14521 aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta tacgaaatat
    14581 ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca gtggcataca
    14641 tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt cttttactgt
    14701 caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc agagcgttaa
    14761 cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata cctaccttga
    14821 gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg atgcgccggt
    14881 attcttcggc ggtaactccg atgctaaagg tatctggaac cacggttctc cgctgtttat
    14941 ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta gcttcggtcc
    15001 gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta ataaagatgg
    15061 tcgccagagc acctggtaca tgggtctggg taccgacatc gatactggtc tgccgatgag
    15121 cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag cgaacgaaaa
    15181 cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg atctgtgggg
    15241 cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt taggggatga
    15301 cagcggtaac gcaatcaacg gtattaagac ccgtaccaat aactctatcg cttccagcca
    15361 tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt actggcacga
    15421 cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact tcaacgttcg
    15481 ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat gaaaatgccg
    15541 ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg ccggatgcgg
    15601 cgcaaacgct ttgtccgacc cacaaaagca cgtaaattca atatattgct gaaat
//
LOCUS       DAZFAH010000102        15516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFAH010000102 DAZFAH010000000
VERSION     DAZFAH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.102"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHJWB_002998"
     CDS             <1..112
                     /locus_tag="ACHJWB_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4314591.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHJWB_002999"
     CDS             171..374
                     /locus_tag="ACHJWB_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4314592.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..1424
                     /locus_tag="ACHJWB_003000"
                     /pseudo
     CDS             374..1424
                     /locus_tag="ACHJWB_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1541)
                     /locus_tag="ACHJWB_003001"
     tRNA            complement(1452..1541)
                     /locus_tag="ACHJWB_003001"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Ser,seq:cga)
     gene            1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHJWB_003002"
     CDS             1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHJWB_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4314593.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2533..2608
                     /locus_tag="ACHJWB_003003"
     tRNA            2533..2608
                     /locus_tag="ACHJWB_003003"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2566..2568,aa:Asn,seq:gtt)
     gene            2920..10902
                     /locus_tag="ACHJWB_003004"
                     /pseudo
     CDS             2920..10902
                     /locus_tag="ACHJWB_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(11164..12216)
                     /locus_tag="ACHJWB_003005"
     CDS             complement(11164..12216)
                     /locus_tag="ACHJWB_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4314594.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12531..13847
                     /gene="shiA"
                     /locus_tag="ACHJWB_003006"
     CDS             12531..13847
                     /gene="shiA"
                     /locus_tag="ACHJWB_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4314595.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAMYLLAGCLISAITALLMKDSQRA"
     gene            13949..15403
                     /gene="amn"
                     /locus_tag="ACHJWB_003007"
     CDS             13949..15403
                     /gene="amn"
                     /locus_tag="ACHJWB_003007"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4314596.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTMTRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4247 a         3695 c         3711 g         3863 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac cacctccaaa
      421 tgtataccca aataaatata gttgtgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaagggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
     1321 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
     1381 aagaaaatat tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag
     1441 tgattgaatt ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg
     1501 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
     1561 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
     1621 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
     1681 cccttccctg gcaggaagca ctatcgatcc cccttttaac gtgtctgacg gaacaggaac
     1741 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
     1801 agggctttga actggattca ttaagaacct gccggatagc acttctattt tgcctacccg
     1861 ttctggaatt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1921 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1981 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     2041 attcttttga tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     2101 cccgtaacgg cgatcgcgcc agcggagttc cctttattcc gttgcgtgag gttgctggct
     2161 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg
     2221 agaatgcggc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     2281 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     2341 aacgtttctg ccaattttat caacaagatc ctttgcagag actgcatcac gctaatgata
     2401 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     2461 ttgaaatgac ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgccc
     2521 cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     2581 actggttcga gtccagtcag aggagccaaa ttctaaaaat tcgcttctta gtgcaatgtc
     2641 actaacctta gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc
     2701 ctcgaccttt atggttgagg gcgttttgct atggcgtcat caccattttc ccctcgatta
     2761 taaaacttga gtaattcagt agtctcccct cttgcaactc acacccaaaa ctgcctaacg
     2821 aaaagttatt aattttcaat catattgcta tcaggattta cattttttcg ctgtgctaga
     2881 aagggcgcat ttatgttagc tcgttcaggg aaggtaagca tggctacgaa gaagagaagt
     2941 ggagaagaaa taaatgaccg acaaatctta tgcgggatgg gtatcaaact acgccgctta
     3001 actgcgggta tctgcctggt aactcaactt gtgttcccta tggctgcggc agcacaaggt
     3061 gtggtaaacg ccgcaaccca acaaccagtt cctgcacaaa ttgccattgc aaatgccaat
     3121 acggtgccct acacccttgg agcgctggaa tcggcccaaa gcgttgccga acgtttcggt
     3181 atttcggtgg ctgagttacg caaactcaac cagtttcgta cgtttgctcg aggttttgat
     3241 aatgtccgcc agggtgatga actggatgtc ccggcacaag ttagtgaaaa taatttaacc
     3301 ccgccaccgg gtaatagcag tggcaacctt gagcaacaga tagccagtac ttcacagcaa
     3361 atcgggtctc tgctcgccga ggatatgaac agcgagcaag cggcaaatat ggcgcgtgga
     3421 tggcctcttc tcaggcttca ggcgcaatga cagactggtt aagccgcttc ggtaccgcaa
     3481 gaatcacgct gggcgtggat gaagatttta gcctgaagaa ctcccagttt gattttctcc
     3541 atccgtggta tgaaacgcct gataatctct ttttcagtca gcatactctc catcgtactg
     3601 acgagcgtac gcagattaac aacggcttag gttggcgtca tttcactccc acatggatgt
     3661 cgggcatcaa cttctttttc gaccacgatc ttagccgtta ccactcccgc gccggcattg
     3721 gcgcggagta ctggcgcgac tatctaaaat taagcagtaa cggctatttg cgactgacca
     3781 actggcgcag cgcacctgaa ctggacaacg attatgaagc acgcccggcc aatggctggg
     3841 atgtacgcgc agaaggctgg ctacccgcct ggccgcacct tggcggtaaa ctggtctatg
     3901 aacagtatta tggcgatgaa gtggccctgt tcgataaaga cgatcggcaa agtaatcctc
     3961 atgccataac cgctggactt aactgtaccc ccttcccgct gatgaccttc agcgcggagc
     4021 aacgtcaggg taaacagggc gaaaatgaca cccgttttgc cgtcgatttt acctggcaac
     4081 ctggcagcgc aatgcagaaa cagcttgacc cgaatgaagt cgctgcacgg cgtagccttg
     4141 caagcagccg ttatgatctg gtggatcgca acaacaacat cgttctggaa tatcgcaaaa
     4201 aagaactggt tcgcctgtcc ctgacagacc ccgtgacagg gaagtcagga gaaatgaaat
     4261 cactggtttc gtcgctacaa accaaatatg ccctgaaagg ctataacgtc gaagccaccg
     4321 ctctggaagc tgccggtggt aaagtggtta caacgggtaa agatattctg gttaccctgc
     4381 cggcgtaccg gttcaccagt acgccagaaa ccgataacac ctggccgatt gaagtcaccg
     4441 ctgaagatgt caaaggcaat ttgttcgaat cgtgaacaga gcatggtagt cgttcaggca
     4501 cctacgttaa gccagaaaga ttcctcggta tcgttaagta cccagacgtt gagcgcggat
     4561 tcccattcaa ccgccacact gacttttatt gcgcatgatg cagcaggtaa tcctgttatc
     4621 gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca tcaccctttc tgactggaaa
     4681 gataatggtg acggaagcta tacccagatc ctgaccacag gtgcgatgtc tggcacgctg
     4741 acgcttatgc cacagctgaa cggtgtggat gcggctaaag cccccgccgt ggtgaatatc
     4801 atttctgttt cgtcatcccg gactcactcg tcaattaaga ttgataagga ccgttatctc
     4861 tccggcaatc ctatcgaggt aacggtagaa ctgagagatg aaaatgacaa acctgttaag
     4921 gagcaaaaac agcaactgaa taccgcagtc agcatcgaca atgtgaaacc tggtgtcact
     4981 acagactgga aagaaaccgc agatggcgtc tataaggcaa cctataccgc ctataccaaa
     5041 ggcagtgggc ttactgcgaa gctattaatg caaaactgga atgaagattt gcataccgct
     5101 ggatttatca tcgacgccaa cccgcagtca gcgaaaattg cgacattatc tgccagcaat
     5161 aatggtgtgc tcgccaatga gaatgcagca aacaccgtct cggtcaatgt cgctgatgaa
     5221 ggaagcaacc caatcaatga tcataccgtc acgtttgcgg tattaagcgg atcggcaact
     5281 tcctttaaca atcaaaacac cgcaaaaacg gatgttaatg gtctggcgac tattgatctg
     5341 aaaagtagta agcaggaaga caacacggtt gaagtcaccc ttgaaaatgg cgtgaaacaa
     5401 acgttaatcg tcagttttgt cggcgactcg agtaccgcac aggttgatct gcagaagtcg
     5461 aaaaatgaag tggtcgctga cggcaatgac agcgccacaa tgaccgcgac agttcgggat
     5521 gcaaaaggca acctgctcaa tgacgtcaag gtcaccttca atgttaattc agcagcagcg
     5581 aaactgagcc aaaccgaagt gaatagccac gacgggatcg ccacagctac gctgaccagt
     5641 ttgaaaaatg gtgattatac ggttacggcc tctgtgagct ctggttctca ggctaatcaa
     5701 caggtgaatt ttatcggtga tcaaagtact gcagccctga ccctcagtgt gccttcaggt
     5761 gatatcaccg tcaccaacac agctccgcaa catatgactg caaccttgca ggataaaaat
     5821 ggcaacccac taaaagataa agaaatcacc ttctctgtgc caaacgacgt cgcaagtcgg
     5881 ttctcgatta gcaacggcgg aaaaggcatg acggatagta acggggttgc aatcgcctcc
     5941 ctgaccggca cgttagcggg cacgaatatg atcacggctc gtctggctaa cagcaatgtc
     6001 agcgatgcac agccaatgac gtttgtggcg gataaagaca gagcggttgt cgttctacaa
     6061 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgaccctgac agcaacagtg
     6121 aaagatcctt ttgataacgt ggttaaaaat ctgtcagtag tcttccgcac ctcccccgca
     6181 gacacgcaac tgagtctgaa cgcgcgtaat actaatgaga acggtattgc tgaagttacc
     6241 cttaagggca cggttttggg tgttcataca gccgaagcca tactgcttaa cggcaacaga
     6301 gatacgaaga tagtcaatat tgcgcccgat gccagcaacg cgcaggtcac cctgaacatc
     6361 cctgcacaac aggtggtgac gaataacagc gacagcgtgc agctgacggc gacggtgaaa
     6421 gatccgtcga atcatccggt ggcgggaata acggtgaact tcaccatacc acaggacgtg
     6481 gcggcaaact ttacccttga aaataacggt attgccatca ctcaggccaa tggcgaagcg
     6541 catgtcaccc tcaaaggcaa aaaagcgggc acgcatacgg ttaccgcaac gctgggtaac
     6601 aataatgcca gcgatgcgca accagtcacc ttcgtggcgg ataaggacag cgcggttgtc
     6661 gttctgcaaa catcgaaagc ggaaatcatt gggaatggcg tggatgagac gactctgacg
     6721 gcaacagtga aagatccttt tgataacgca gtaaaagatc tacaggtcac cttcagtaca
     6781 aatcccgcag atactcaact tagtcagagc aaaagcaata ctaacgacag tggtgtggcc
     6841 gaagttacct ttaagggcac ggttttgggt gttcatacag ccgaagccac actgcctaac
     6901 ggcaacaacg atacgaagat agtcaatatt gcgcccgatg ccagcaacgc gcaggttacg
     6961 ctgaacatcc ctgctcaaca ggtggtgacg aataacagcg acagcgtgca gctgacggcg
     7021 acggtgaaag atccgtcgaa tcatccggtg gcgggaataa cggtgaattt caccatgcca
     7081 caggacgtgg cggcaaactt taccctcgaa aataacggta ttgccatcac ccaggccaat
     7141 ggggaagcgc atgtcacgct caaaggtaaa aaagcgggta cgcatacggt taccgcaacg
     7201 ctgagtaata acaataccag tgattcacag ccggtaacgt ttgtggcgga caaaacctcg
     7261 gctctggttg ttcttcagat atcaaaaaat gagatcacag gtaatggcgt cgatagcgca
     7321 acgctaactg caacggtcaa agatcagttc gacaatgagg tgaacaatct tccggtaaca
     7381 ttcagcacag cttcttcagg cctcaccctg accccagggg aaagtaatac caatgagtct
     7441 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagactgt tactgcatca
     7501 ctggctaata ctggtgccag cgacaacaaa actgtgcact ttattggcga cacaacggcg
     7561 gcaaaaatta tcgagttgac gcctgaccca ggcagcataa tcgccggtac cccacagaac
     7621 agcaccggta gcgtaatcac cgctacagtc attgataata atggctttcc ggtgaaaggt
     7681 gtgactgtga acttcaccag caacgcagcg acagccgaaa tgacgaatgg cggccaagcc
     7741 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
     7801 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
     7861 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcaccttgtt acaggcactt
     7921 cttgatacag tctccgcagg cgacactacc aatctgtata ttgaggtgaa ggataattac
     7981 ggcaacggtg taccccagca ggaggtaacc ctcagcgttt caccaagtga aggcgtgact
     8041 cccagtaata acgctatata tacgaccaat cacgacggca atttttacgt aagctttacc
     8101 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatacaa
     8161 caaacggtga cctatgtgcc gaacgtcgcg aatgccgaaa tcacgctggc agcctcgaag
     8221 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
     8281 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgtgaaggcg
     8341 aacttcacgc tgagcgatgg cggtaaagcg gttactgata ctgaaggcaa agcgaaagtc
     8401 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgatggc tggcggtaag
     8461 agtgagcagt tggtggtgaa ttttattgcg gatacgctca cagcgcaggt taatcttaac
     8521 gttaccgagg acaactttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
     8581 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
     8641 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
     8701 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
     8761 aattatggtg tcagtgatac gaaacaggta actttgattg ccgatgctgg taccgcaaaa
     8821 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaggg cgcgaccatg
     8881 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt tagtttccgc
     8941 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcgctgaa
     9001 attcttgtga caagcaccga ggtcggactg aaaacagttt cagcctctct ggcagataaa
     9061 cctactgaag tcatctcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
     9121 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
     9181 cacgtcaacg accagtttgg taatccgatt cttaatgaat ctgtaacatt cagtgcagag
     9241 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
     9301 gaggtcacta tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
     9361 ggatcctctt atgagaagga tctggtggta atcgatcaaa aactgacact ctcggcgtcc
     9421 agcccgctta tcggtgtcaa ttccccaaga ggtgcaactc tgacggcaac gctaacttct
     9481 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
     9541 acgttaagtg gcggaaaagt gagaaccaac tcttcaggtc aggctccagt cgttctgacc
     9601 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggtgt aacaatacag
     9661 acacagacaa tcgtgaaagt cactggcaac tcaagcaccg cccatgttgc tagctttatc
     9721 gctgctccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
     9781 gaggatggca gtggtaacct gatcgaaggt ttaactgtgt acttcgcctt aaaaagcggc
     9841 tctgccacat taacgtcatt aacagcggtg accgatcaaa acggaatcgc gacaacaagc
     9901 gtgaaaggag cgatgacagg tagcgtcacg gtaagcgcag tcacgaccgc tggtggaatg
     9961 caaacagtag atataacgct ggtggctggc ccggcagaca cctcgcagtc cgtccttaag
    10021 aacaatcggt catcattgaa aggagacttt accgatagtg ctgagctaca tcttgttctg
    10081 cacgatatat caggcaatcc gatcaaagtt tctgaagggc tggaatttgt gcagtcaggt
    10141 accaacgtgc cctatgtgca agttagtgca attgactaca gtaaaaattt ctcaggcgag
    10201 tacaaagcca ctgttacagg cggcggagag ggtatcgcaa cgctgatccc tgtattgaat
    10261 ggtgttcatc aagctggtct gagtaccaca atacaattca ctcgcgcaga agacaaaata
    10321 atgagcggta cagtgtcagt caatggtact gacctaccga caactacatt cccttcgcag
    10381 ggattcaccg gggcgtatta tcagttgaat aatgacaact ttgcccctgg aaaaacggcg
    10441 gctgattatg agttttcaag ctctgcctcc tgggtcgatg ttgatgctac cggtaaagtg
    10501 acatataaaa atgtcggcag caattgggaa aggattacgg cgacgccaaa atcaggaggc
    10561 cctagctatg tatacgaaat ccgtgtgaag agttggtggg taaacgccgg cgatgctttc
    10621 atgatataca gccttgctga aaatttttgc agtagcaatg gctacacact tccccgtgca
    10681 gatcatttaa accatagtcg ttcccgaggc atcgggtcac tgtacagtga atggggagat
    10741 atggggcatt acacgactga ggctggtttt caatcaaata tgtattggtc atctagtccc
    10801 gcaaactcaa acgaacaata cgtagtttcc ctggcaacag gtgatcaaag cgtatttgaa
    10861 aagcttgggt ttgcttatgc gacatgttat aaaaacctct gattttccgt tataatttct
    10921 taaagagtgc tccattcggg gcactctttt ttacattccc tctatagagc ttgctcttct
    10981 ctcaccgaaa atgaataaat aaaaatgcga taccgccaga ttcgcgttcg gtgtgactat
    11041 gatcgtatcc ggtgagtaca gctttgatcc aaaggctggt tttctgtgat gattgaccag
    11101 cctcagagta ttgatgacac caccgttcat aacagatcgg cattactatg cataagtact
    11161 gtctcatatt ctcgccaaaa cactttttaa tgcctttgcg aattcatcca gacttatatt
    11221 gtcaattttt tgcccaacga cgactgactc cgaaacatcg gccattgcgg gccatcgcgt
    11281 tttgtaatca gcataaattg caacaacggg ggtattatag gcacttgcca tatgtaatat
    11341 tgaagtgtca ggagttattg cgatataagc atcttttatg attgctgctg aacgtttaat
    11401 cgagggtgat aaggataaac ggtagacatt attataacaa tccactaata ccctggcttt
    11461 atcttcccct tttggtccat gaacgatcac tattggtata tctgtttcac tttgaatttt
    11521 tgctattaga ttttctgcaa tcgatagtga aaatgtacgt tcctgcgagc taccttctaa
    11581 attaagcgca atgtaagatc ctaacgatcg catttcctcg cgcacctcat cgagtacatc
    11641 ctcacttagt ggcaattcat atattggcct gactgctgga aaacccgcct cacgcatcat
    11701 aaatgcccat gtcatgggta cgggagcccg gagattctga tccatccggg acgcgttctt
    11761 acacacaggg gaatagcact tcatggttaa accaacaacc tgaaaattcg tttttgcttt
    11821 caactgactg ataaataaca tcgttttcag gttcttttta cgcatcgcct ctatacaaag
    11881 atccggcgta ccgtattgct gtgttatgtt cttcgctaaa tcttttattt cttttaatgt
    11941 tgcgtgatcc tgcatagtca ttgtgactaa tgttaattta gtctgttcaa gtttgagcac
    12001 attaaagact tctaaattaa ttgtcgacgt aacaattaat agatgcttaa ttttatgcaa
    12061 ttcaagcgcc cgaataacag gaaagatggc catagcatcg ccaatctgat cgggaatatg
    12121 gatgacaaca aagtctgttt tttcaatatt gaaattataa gctttataat cgtagtagct
    12181 aaatgcaata cgtctcaaca atgatactaa aaacataact aacctcgcct ccctactggt
    12241 tataatgcaa tgcagtctat cagaatcatc agggtgccat tttgtgcata tgcggacttt
    12301 tatgtttcat atctcgaacc tgtgggtcct ctgcttaatc cttaaacaac accagcaact
    12361 cccgcgcttt catcttccag cgaatttttc atgttgccgc taatcagcca taaaatcatt
    12421 tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg
    12481 agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca
    12541 cgctcatctc cactcgtccc gatgaaagga cggtttcgtt aagtcgcgcc cgacgagctg
    12601 cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatca
    12661 ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc
    12721 tcgccgcatt tgctaccttt ggcgtcggat tccttttccg tccgctcggc ggtgtcattt
    12781 tcggtcactt tggcgaccga ctgggacgta agcgcatgtt aatgctgact gtctggatga
    12841 tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg
    12901 cacctatttt actggtgacg ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg
    12961 gaggcgcggc gttgctttcc gttgaaagtg caccgaaaaa taaaaaagcc ttttacagta
    13021 gcggtgtaca agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga
    13081 tcagtatgat gacgactgac gaacagtttt taagttgggg ctggcgtatt cctttcctgt
    13141 ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg
    13201 aattcgaaca acagcaacat aatcaagctg ccgcgaaaaa acgcatcccg gttatcgaag
    13261 cgctgttacg acatccaggt gctttcctga agattattgc gctacggctg tgcgagttgc
    13321 tgacgatgta catcgttacc gcctttgcac ttaattattc aacccagaat atggggttac
    13381 cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc
    13441 cctgttttgc ctggcttgcc gatcgttttg gtcgccgcag ggtttatatc acaggtgcgt
    13501 taatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct
    13561 ggatagtttt cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc
    13621 aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctatagtggc gctggagtcg
    13681 gttatcaggt tgccagtgtg gttggcggag gatttacacc ttttattgcc gctgcactca
    13741 tcacttactt tgccgggaac tggcatagcg tcgccatgta tttgctggct ggatgtctga
    13801 tttctgcaat aaccgctttg ttgatgaaag acagtcaacg cgcttgatag cctggcgaag
    13861 atcatccgat cttcgcctta cacttttgtt tcacatttct gtgacatact atcggatgtg
    13921 cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc
    13981 tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagtcgcat tacgcaacgc
    14041 cattggcaac tatattacaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct
    14101 ttttgtctat ccatcactga ccgtaacctg ggacggtagc acaaccaatc cccccaaaac
    14161 gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc accacgatga ctcgccctac
    14221 tctctttcgt tcgtatctta atgaacaact tacgttgctg tatcaggatt atggtgcgca
    14281 tatctcggtg caaccctcgc agcatgaaat cccttatcct tatgtcatcg atggctctga
    14341 attgacactt gatcgctcaa tgagcgctgg gttaactcgc tatttcccta caacagaact
    14401 ggcgcaaatt ggcgatgaaa ctgcagacgg catttatcac ccaactgaat tctccccgct
    14461 atcgcatttt gatgcgcgcc gcgtcgattt ttccctcgcg cggttgcgcc attataccgg
    14521 tacgccagtt gaacattttc agccgttcgt cttgtttacc aactacacac gttatgtgga
    14581 tgaattcgtt cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct
    14641 ttcttgtgct ggcgggaact ggatcaccgc cgaaaccgaa gcgccagaag aagccatttc
    14701 cgaccttgca tggaaaaaac accagatgcc cgcgtggcat ttaattaccg ccgatggtca
    14761 gggtattact ctggtgaata ttggcgtggg accgtcaaat gctaaaacca tctgcgatca
    14821 tctggcagta ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga
    14881 aagtcaggcc attggcgatt atgtacttgc acacgcttat ttacgcgatg accacgttct
    14941 tgatgcggtt ctgccgcccg atattcctat tccgagcatt gctgaagtgc aacgtgcgct
    15001 ttatgacgcc accaagctgg tgagcggcag gcccggtgag gaagttaaac agcggctacg
    15061 tactggcact gtagtaacca ctgatgacag gaactgggaa ttacgttact cagcttctgc
    15121 acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc
    15181 gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt
    15241 gcatggcgag attaaacttc ccggccaggc taaccgtttt tatgaaggtg ctatttccga
    15301 acacctgcaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca
    15361 ttcacgtaaa ttacgaacct ttaatgagcc gccgttccga taataagttc tgaaccttct
    15421 tatcaaaaat agatgtgaag gattaagtaa gacatctggc aaatagcccg caataaacgc
    15481 gggctgtttg cttccttggg cggatacgag ttttat
//
LOCUS       DAZFAH010000103        15486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFAH010000103 DAZFAH010000000
VERSION     DAZFAH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.103"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(224..1030)
                     /gene="trpA"
                     /locus_tag="ACHJWB_003008"
     CDS             complement(224..1030)
                     /gene="trpA"
                     /locus_tag="ACHJWB_003008"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4314597.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1030..2223)
                     /gene="trpB"
                     /locus_tag="ACHJWB_003009"
     CDS             complement(1030..2223)
                     /gene="trpB"
                     /locus_tag="ACHJWB_003009"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4314598.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2235..3596)
                     /gene="trpCF"
                     /locus_tag="ACHJWB_003010"
     CDS             complement(2235..3596)
                     /gene="trpCF"
                     /locus_tag="ACHJWB_003010"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4314599.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3597..5192)
                     /gene="trpD"
                     /locus_tag="ACHJWB_003011"
     CDS             complement(3597..5192)
                     /gene="trpD"
                     /locus_tag="ACHJWB_003011"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4314600.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5192..6754)
                     /gene="trpE"
                     /locus_tag="ACHJWB_003012"
     CDS             complement(5192..6754)
                     /gene="trpE"
                     /locus_tag="ACHJWB_003012"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4314601.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6776..6869)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6846..6890)
                     /gene="trpL"
                     /locus_tag="ACHJWB_003013"
     CDS             complement(6846..6890)
                     /gene="trpL"
                     /locus_tag="ACHJWB_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4314602.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7028..7909
                     /gene="rnm"
                     /locus_tag="ACHJWB_003014"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             7028..7909
                     /gene="rnm"
                     /locus_tag="ACHJWB_003014"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4314603.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7906..8526
                     /gene="yciO"
                     /locus_tag="ACHJWB_003015"
     CDS             7906..8526
                     /gene="yciO"
                     /locus_tag="ACHJWB_003015"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4314604.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8627..9499
                     /gene="rluB"
                     /locus_tag="ACHJWB_003016"
     CDS             8627..9499
                     /gene="rluB"
                     /locus_tag="ACHJWB_003016"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4314605.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9539..10129)
                     /gene="cobO"
                     /locus_tag="ACHJWB_003017"
     CDS             complement(9539..10129)
                     /gene="cobO"
                     /locus_tag="ACHJWB_003017"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4314606.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10126..10884)
                     /gene="yciK"
                     /locus_tag="ACHJWB_003018"
     CDS             complement(10126..10884)
                     /gene="yciK"
                     /locus_tag="ACHJWB_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4314607.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11104..12153
                     /gene="sohB"
                     /locus_tag="ACHJWB_003019"
     CDS             11104..12153
                     /gene="sohB"
                     /locus_tag="ACHJWB_003019"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4314608.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12189..12440)
                     /gene="yciN"
                     /locus_tag="ACHJWB_003020"
     CDS             complement(12189..12440)
                     /gene="yciN"
                     /locus_tag="ACHJWB_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4314609.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12820..15417
                     /gene="topA"
                     /locus_tag="ACHJWB_003021"
     CDS             12820..15417
                     /gene="topA"
                     /locus_tag="ACHJWB_003021"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4314610.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3758 a         3929 c         4153 g         3646 t
ORIGIN      
        1 acctgaggaa taagtgactt agagaaaatt attgatttta ctggtgttat gttgcgggat
       61 taatttgttt gattatatta ctgttgggcg gaaaaatgac gtaagttgac gttcgaccgg
      121 ggtaagcgaa acggtaaaaa gataaatatt aaatgaattt aggatttttc cggcttcatt
      181 aaagaaagtt aaaatgccgc cagcggaact ggcggctgtg ggattaactg cgcgtcgccg
      241 ctttcatcgg ttgtacaaaa actttcagtg ccgccagcat tttctctggc tcattaatat
      301 gttgctcgat gattttaaca atggccgaac cagaaatcgc gcccgcagct cctgcatcaa
      361 tcgctgcttt tacctgatcc ggggcggaaa taccaaatcc ctgcaatgga ggtgcagcgt
      421 tgtactcttt cagcttcgca accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc
      481 cggtcacgcc tgctcgtgac agcaaatagg tgtaaccacg accgtaagag gctatctggc
      541 gcagcaggtc gtcatcggca tttggcgggc agatgaagat aggtgcgaca ttatgacgca
      601 acgcggcctg gcggaagggc gcggactctt caactggcac atcggcaacc agcaccgaat
      661 cgacgccgac tttttcgcac tgggcataaa actcatcaat gcctttgtta aacaccagat
      721 tggcatacat caacaggcca atgggaatgg tcgggtgttt ctggcgaatc agtgccagca
      781 tttcaaaaca ttgtgccgga gtcacacctg ccgcaaaggc gcgcagagtg gcgttttgaa
      841 tcgtcgggcc atccgccagt gggtcggaga aggggatacc taactccagc gcgtcagcac
      901 cggcttcaat tagcgtatcg ataattttca atgactgctc aatgcccgga tcaccgagcg
      961 tgacgaaagg aacgaatgcg ccttctttgc gctccttcaa ctgggcaaac agagattcgt
     1021 agcgttccat cagatttccc ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt
     1081 atcgccgcga ccggaaaggt taaccaccag tagctgctct ttatccgggt tttcgcgcat
     1141 cattttcaac gcatgggcca gggcgtggga ggattccagc gccgggatga tcccttcgtg
     1201 caggcacagc gttttgaagg cttcaagggc ttcatcatcg gtaatagaca cgtaatcagc
     1261 gcgtccagtg ctgttaagat acgcgtgttg tgggccgaca gacgggaaat ccagtccggc
     1321 ggagatggag taagattctt caatctgccc gtcttcggtt tgcatcatcg gcgctttcat
     1381 accgaaatag atacccacgc gaccatgttt tagcggtgcg ccgtgctcgc cagtttcgat
     1441 accgtgacca cctggctcca caccaatcag gccgacgttg gtttcattga tgaaatcagc
     1501 aaacatgccg atggcattcg aaccgccgcc aacacaggca ataacggcat ccggcaggcg
     1561 accttctctt tccagaatct gcgctttggt ttcttcgcca atcatccgct gaaactcacg
     1621 cacaatggtc ggataaggat gcgggccagc tgcggtgccc agcatatagt gcgcggtttc
     1681 gtaactaccg gaccagtcgc gcagcgcctc gttacaggca tctttcagcg tcgcggaacc
     1741 gctatgcacc gggatcactt ccgcacccat taagcgcata cgaaaaacgt taggcgactg
     1801 gcgttcaacg tctttggcac ccatataaat acggcatttc aggccgagca gggcgctggc
     1861 aagggccgac gccacgccat gctgaccggc accggtttcg gcgatgattt cggttttacc
     1921 catccgcttc gccagcaacg cctgccccag cacctggtta gttttatgcg cgccgccgtg
     1981 cagcaaatct tcacgcttga gatacagcgt ggtgttcgtc ccggctgtaa tgttctggca
     2041 tttggtcagc gcggttggac gcccggcata gtttttcagc aggtcgttga actgagcctg
     2101 aaattcagga tctttttgcg cactgacaaa agcttcttcc agctggcgca gagcaggcat
     2161 caggatttgt ggcacgtaca tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt
     2221 cattgttcct ttccttaata tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg
     2281 tctttgatgc ccggttgcga ctctacagca gaattaaaat caagtccggc gcagccggtt
     2341 tgtgccgctt ccacgcagtt atctgcgcct aagccccccg ccagcagaac gttgccaagc
     2401 gattgaccat ttaatagtga ccagtcaaaa cgttgcccgc ttccaccctg gccgttgtct
     2461 aaaacatatt tatcaacgtg ctgaaactcg cgggcgggca gggtttcacc gacgcttaat
     2521 gctttccaga tggcaacatg tgctggcaga gcttcacgca gcgtatcgat atacagctgt
     2581 tcttcattac catgcagttg cactgccgcc agcgataaca ccttagcttt gtccaccaca
     2641 tcggcaatat cgtgattgcg gaacacgcca acatactgca acggtgccgc agccatcact
     2701 tcctgcgcct gttcaacgtt gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg
     2761 taaatcgcgc ccgcgtcata agctgcttta gcatcttgcc cacgcgtcag gccacatact
     2821 ttattctcac ccagcaacac ccggcgcacg gcggcgtgca aatcgtcatg ggccatcaac
     2881 gccgaaccaa tcagaaaacc gttagcgaag tggcttaact cgcgcacctg agcgtaagta
     2941 ttgatgccgg attcgctgat taccgtcacg ttgtgcccca gtttcggcgc aagctcgcgg
     3001 gtacggttga gatcaatcga caaatcacgc agatcgcggt tgttgatgcc aacgaccttt
     3061 gcccccaatg caatggcacg ctccagttcc tcttcattac tgacttcggt cagcacaccc
     3121 atctccagac tgtgggcgac ggcggcaagc tggcgatatt gttcgtcatc cagtactgaa
     3181 agcattaata agcaggcatc agcctggtaa tagcgcgcca gatagatctg gtaaggatcg
     3241 ataatgaagt ctttacataa aatcggctgc ggggcgattt ggctgacgat ggggaggaaa
     3301 tcaaagctcc cctgaaaata tttctcatca gtcagcaccg aaattgccga agcgtaatgt
     3361 ttataaatgg cggcaatgcg tgctggatcg aaatcatcac ggatcacgcc ttttgacggc
     3421 gacgctttct tgcactccag aataaacgcc gtgcgcgcac cctgtagcgc atcataaaaa
     3481 tgtcgcgtgc tcggctgaac ctcattctga aaactggcca gcggttgctg ctgtttgcgg
     3541 gtttctaccc aaatcgcctt gtctgcgacg attttcgcta aaacggtttg catcatttac
     3601 cctcgtgccg ccagtgcggt aactctgtcg taagcggaac cactgcgcag tacctcaaga
     3661 acggtttgcg cattggcttg cagatcttca tggccatgca ggcgcattaa catggcgacg
     3721 ttcgcagcga cggctgcttc atgggcggcg tcgcctttac cttgtaacaa acgtgttaaa
     3781 atgtcacggt tttcttccgg tgttccgcct gccagttgct cctggtggta gggtgtcagg
     3841 ccaaaatctt cagcggtcaa ttgatagctc ttaatttcgc cgtcatgcag ctcggcaacg
     3901 attgtcggcg cgtgtaatga aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt
     3961 tgatacccca gcacgcgcaa ggtttcggca atcggcagca ccagttccgg actataaaca
     4021 ccaattaacg ccagcggcgg atgcgccggg ttaatcaatg gccccagcac attgaacagg
     4081 gtgcgggttt ttagttgctg gcgaaccggc attgcatggc gaaatccggt gtgatatttc
     4141 ggtgcaaaga ggaaacatac gcctaactca tccagcgcct ggcgcgattt atcggcgttc
     4201 atatcaagat taataccgaa cgccgccagc agatcggacg aaccagattt actggagacg
     4261 ctacggttgc cgtgtttcgc cactttcagc ccacaggccg cggcgacaaa cgcactggcg
     4321 gtagaaatat tgatactgtt gctgccgtca ccgccagtac cgacgatatc agcaaacaga
     4381 taatccgggc gcgggaacgg cgctgcgttt tccagtagcg cggttgctgc cccggcgatc
     4441 tcgttcgggt gctcaccgcg aattttcatg ctcaccagcg ccgccgccag ttgttccggc
     4501 ttcagctcgc cacgcaccac cgctgaaaac agctggtggc tttcttgttg gctaagcgtc
     4561 tgcgcctgat acagtttttc cagaatcggt tgcagcgtgt tggctggctc tagtttctgc
     4621 tgcgcccagg ccagcgtttg ttccagcagg cgagcgccct gggtggtgag aatggattcc
     4681 ggatggaact ggaatccaca aacgcgatcc gcatcgtgac gtactgccat caccatgcca
     4741 ttaaaatggg cgttgatggt taaaccggcc ggaatgttac tgccaaccag cgagtgataa
     4801 cgcgccaccg gcagcgggtt tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg
     4861 ctcgacgctt taccgtgaag aatttcgccc gcctgaccga catagccccc gtaagcttcg
     4921 acaattgcct gatgtccgag gcaaatgcca ataatgggca gcttgccacg caagcgggtg
     4981 aggagttccg gcatacaacc ggcttcgctc ggcacaccgg ggccaggaga aagcatcagt
     5041 accggattgc tcatggtcgc caggcgttca attaaggtct gcgccggaat atggttgcgg
     5101 taaatcacca cgttatgccc attgctgcgc aactgatctg ccaggttgta cgtaaaagag
     5161 tcgatattat cgagcagcag tatgtcagcc atcagaaagt ctcctgtgca tgatgcgcgg
     5221 tggcaatagc gcgcagtaca gcgcgggctt tattacgggt ttcgtcggct tccgactgcg
     5281 gaatagaatc aaggactacg ccagcaccgg cttgcacggt ggcgataccg ttttccacca
     5341 gcgccgagcg gatcacaatg caggtgtcga gatcgccgtg cgcggtgaaa taacctaccg
     5401 cgccgccgta gctgccgcgg cgacgacctt ccgcctcggc aattaactgc atggcgcgca
     5461 ctttcggcgc accgcttaac gtccccatat tcatacaggc gcggtaagcg tgtagggcgt
     5521 cgagatcgtg gcgcagttca ccgaccacgc gggagaccag gtgcatcacg taagagtaac
     5581 ggtcaacttt ggtgaggtcg gcgacgtagc ggctgccggg ggtgcaaatg cgtgccagat
     5641 cattacgggc aagatccacc agcatcagat gttcagaaag ctctttatga tcggtacgca
     5701 tttccagttc gatgcggctg tcgaggtctc tgtccagcga gccatcggca cgacgaccgc
     5761 gtggacgtgt tccggcaatc gggtagatct caatctggcg gctggtggcg tcatacttga
     5821 gcgaactttc cggcgacgcg ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt
     5881 acgggctggg attactcttt ttcagcacgt aataggccgc cagcggtgac gggcagggca
     5941 gagagaaacg gcgagatggc accacctgga aaatttctcc agcgcgaatc gctttttgca
     6001 acaaacgcac tacgccaccg aactcttcat cgctctgatt acattcacaa cgcatatgcg
     6061 gcacggaaac caccggcagc ggcggcgcgg cttcggtcag ttgctgacgt agttcgttca
     6121 ggcgagcagt gagacgttgt ttttcttctt cattcggagc aaacaggctg gcctgaatac
     6181 gggtgctttt tttctgatgg tcaatcacca tcagagtttc agcgagataa aaacagaaat
     6241 cagggcagtt attttccgct gacagttgcg gtaaatcttc aaatcccgcc acaaggtcat
     6301 aagagaacag gccgccgaag aacattgctt ctcgttcttc cttcggtaca ttcaacagat
     6361 tctgcaataa acggaaagcg tcaaaaaccg aaagggagca taagcgagcg tcttcatcca
     6421 gcagtggact gacagggggg aagcgcagca cacggcagtt tggtaattgt tcattttcca
     6481 cacccgcagg cagggcgtta tccagtagtg tcagcagggc ttcgccgttg ccggaaagtg
     6541 cctggattgt gacagtgtca cctaaagctg taatgcgcag cgcactgtct accagcagca
     6601 ggctttttaa atcatctttg ctgtcgatat ctgcggattc cagcagcagc gttgccggac
     6661 gatccccaca caactggtga aaaagcgcgg tgggattgtc gcgataagcg ccttcgcagg
     6721 ttagcagttc gagagtcggt ttttgtgttt gcattgttat tctctaattt tgttcaaaaa
     6781 aaagcccgct cattaggcgg gctgggtatc tgattgcttt acgcatggtg aatacactgc
     6841 ccgtttcagg aagtgcgcca ccaacctttc agtacgaaaa ttgctttcat tgtcgatacc
     6901 ctttttacgt gaacttgcgt actagttaac tagttcgatg attaattgtc aacagctcat
     6961 ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga
     7021 gtgcgccttg agcgacacga attatgcagt gatttacgac ctgcacagcc ataccacagc
     7081 ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg
     7141 caccctggcg atcaccgacc atgacaccac agctgcaatc gcgccagcaa gagaggaaat
     7201 ttcacgttcg ggactggcgt tgaatcttat tcccggcgtg gaaatttcca cggtctggga
     7261 aaatcatgaa attcatattg tcgggctgaa tattgatatt actcatccgc taatgtgtga
     7321 gttcctcgcg cagcagacag aacggcgcaa tcagcgggcg cagctgattg ccgaaagact
     7381 tgaaaaagcg caaatacctg gggcgctgga aggcgcacaa cgactggcgc agggcggcgc
     7441 agtgacgcgc ggtcattttg cgcgtttcct tgttgagtgc ggcaaagcca gttcaatggc
     7501 ggatgtcttt aaaaagtatc tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg
     7561 tacaatagaa caagctattg atgtcattca tcattctggc ggtaaggcgg tgctagctca
     7621 tcctgggcgg tacaatctta gtgctaaatg gctgaaaaga ttggttgcgc attttgccga
     7681 acaccacggt gacgcgatgg aagtcgcgca gtgccagcaa tcgcccaatg aacgtaccca
     7741 gctggcggcc cttgcgcgtc agcatcattt atgggcatca caaggatctg attttcatca
     7801 gccatgcccg tggatcgaac tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt
     7861 ctggcagcta tgggaacagc cgcagaacac cacagagagg gaattatgag ccagtttttt
     7921 tatattcatc ctgataaccc acagcaacgt ctgatcaacc aggcggtgga gatcgtgcgt
     7981 aaaggcgggg tgattgttta tccaactgat tccggctatg cgctcggctg taaaattgaa
     8041 gataaaaacg cgatggagcg tatttgtcgt attcgccagc tgccggacgg tcacaacttt
     8101 accctgatgt gtcgcgatct ttctgaactc tcgacctatt catttgttga taacgttgcg
     8161 tttcgtttaa tgaaaaacaa cacgccgggc aactatacct tcatcttgaa ggggacgaag
     8221 gaagtgccac gccgtctgtt gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccttct
     8281 aacccgattg cgcaggcgtt gcttgaagca ctgggcgaac cgatgctttc cacttcgcta
     8341 atgctgccag gcagcgaatt taccgaatcc gatcccgaag aaattaaaga ccgtctggaa
     8401 aaacaggtgg atttgattat ccacggcggc tatctcggac agaaaccgac aacggttatt
     8461 gatcttaccg atgatacgcc ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc
     8521 ttataagtgc gtaactctgt atactacgcg gccttaaaat ggcgctatac ggcgccattg
     8581 attctcctgt tcgacgcctg tgaaggcgac accaaaagga agctctatga gcgaaaaatt
     8641 acagaaagtg ctggcccgcg ctggccacgg ctcccgccgt gaaatcgaat ctattattga
     8701 agccggtcgt gtgagtgttg atggcaaaat tgctaaactc ggcgatcgcg ttgaagttac
     8761 ccctgggctg aaaattcgta tcgatggtca tctgatttcg gtacgtgagt ccgctgaaca
     8821 aatttgtcgc gttctggcct attacaaacc ggaaggtgag ttgtgtaccc gtaacgatcc
     8881 ggaagggcgt ccgacggtgt ttgaccgtct gccaaagctg cgtggcgcac gctggattgc
     8941 cgtaggtcgt ctggacgtta acacctgtgg tctgctgctg ttcaccaccg atggtgaact
     9001 ggcaaaccgt ttaatgcatc caagccgtga agttgaacgt gaatacgccg tgcgtgtatt
     9061 tggtcaggtt gacgacgcga aactgcgtga tttgagtcgt ggcgtgcaac tggaagatgg
     9121 tccggcagct tttaaaacca tcaagttcag cggcggcgaa gggatcaacc agtggtacaa
     9181 cgtgactctg accgaaggcc gtaaccgtga agttcgtcgt ctgtgggaag cggttggtgt
     9241 gcaggtgagc cgcctgatcc gtgttcgtta cggtgatatc ccactgccaa aaggtctgcc
     9301 acgcggtggc tggacggagc tggacctcgc ccagactaac tatctgcgcg aactggtgga
     9361 gcttccgcca gaaaccagct ctaaagtcgc tgtagaaaaa gaccgtcgtc gcatgaaggc
     9421 taatcagatt cgtcgtgcgg tgaaacgtca cagtcaggtg agcggcggtc gtcgttctgg
     9481 cgggcgtaat aacggttaat caaagtatgc cggacgtcat atccggcatt tttacagatt
     9541 aataatcaat ccctatttgc gccttaacac cggcatcaaa cgcatgtttg atggggcgca
     9601 gctcacttac cgtatccgcc agttcaagaa tatcccgatg acagcctcga cccgtgataa
     9661 tcaccgtctg ttgatgtgga cgttcattta acgcctgcac cacttcttcc agtggcaaat
     9721 agtcatacgc caccatatac gtcagttcat caagcaaaac catatccagt gaggaatcag
     9781 caagcatccg ctttgcatgt tgccagactt cgcggcaggc ggcggtatca gactcgcggt
     9841 tttgtgtatc ccaggtaaag cccgttgcca tcacctgaaa ctcaacgcca tgtggctcca
     9901 gcagattgcg ttcgccatta ggccaggtgc ctttaataaa ctgcacgacg cccacttttt
     9961 ttccgtgacc aactgcgcgt gttgccgtac caaatgccgc ggtggttttg ccttttccat
    10021 tgccggtaaa gacgatgata ataccgcgtt catcctgggc ctgggccaca cgagcatcta
    10081 ctttttcttt cactcgctgc tgacgctgtt ggtagcgttc atcactcatt gggaaattcc
    10141 tggtttacgg cccggttggg cgtcaaaggt catgccggtt ttacggcggc tgtcatcgcc
    10201 catcagccag aggtagagcg gcatgatatc agcgggtgtt ttaagtttct gtggatcttc
    10261 ggtcgggaag gcgctggcac gcattgcggt gcgcgtaccg cctgggttaa tgcagttgac
    10321 gcgcaggcgc tgctgatatt catcggccag tacctgcatc atcccttcgg tggcaaattt
    10381 cgacgctgca tatgcgcccc agttggctcg tccctgacgt ccaacgcttg atgaagtaaa
    10441 gaccagtgaa ccggcgtccg atttgagtaa taaaggaagc agtgcctggg tgagcataaa
    10501 ggtggcatta acgttgacct gtatgacgtc ctgccagacc tgtggatttt gttcgctcat
    10561 tgggcaaaca tcgccgagca atccggcatt atgcaaaaca ccatccagac gcggataatt
    10621 aacggtaatg cgctgtgcca gttgttggca atcttcggac gtgcaggtca gcaaatcgag
    10681 aataaaccac tgtggctgac gtccagtttc ttcgtttatg tggctggcta cctgacgtaa
    10741 tttttcttca ttacggccca acagaatcac tgtcgcacca tagcgtgcat acgtcatcgc
    10801 ggcttcacgg ccaatcccat cgctggctcc cgtcaccagg ataatgcgat cattgagtaa
    10861 atcttgtttt ggctggtaat gcatggctac tcctcaacga cgttgtctgt cgcgctgcca
    10921 gtgtacttta tgactttggg gctttatgcc tgaaacagaa gggcatttca atcagcctgg
    10981 ggcgataaca ccgcaaaatg gatactttgt catactttcg ctgcaataac atctctgcga
    11041 gacggcttaa catgcctgtt gtaaactgtg agccaaagcg ttgtttaacc aaggtgggga
    11101 ctcgtggaat tgttgtctga atatggtttg tttttggcga aaatcgttac cgttgtgcta
    11161 gcgattgcgg cgattgccgc cattattgtc aatgttgctc aacgtaataa acgccagcgt
    11221 ggcgagttac gggtcaacaa tctcagcgaa cagtataagg agatgaaaga agaactggcc
    11281 gcggcgctga tggactcaca tcagcaaaaa cagtggcaca aagcgcagaa gaaaaagcac
    11341 aagcaagaag cgaaagcagc aaaagcgaaa gccaaactgg gcgaggtggc aactgacagt
    11401 aaacctcgcg tctgggtgct ggattttaaa ggcagcatgg acgcccatga agtgaactcg
    11461 ctacgtgaag agataacggc ggtactcgca gcattcaaac cgcaggatca ggttgtgctc
    11521 cgtctggaaa gccctggtgg catggtgcat ggttacgggt tggcggcttc gcagctgcag
    11581 cgtctgcgtg ataaaaacat tcctttaact gttacggtag acaaagtcgc tgccagcggc
    11641 ggttacatga tggcctgtgt ggcggacaaa attgtttccg caccgtttgc tattgtgggt
    11701 tccattgggg tggtggcgca aatgcccaac tttaaccgct tcctgaaaag caaagatatt
    11761 gatatcgaac tgcacactgc cgggcagtat aagcgtactc tgacgttgct gggtgaaaat
    11821 accgaagaag ggcgggagaa attccgcgaa gagttgaacg aaacgcatca gttgtttaaa
    11881 gattttgtga agcgtatgcg tccgtctctg gatattgaac aggtggcaac gggtgaacac
    11941 tggtacggac aacaggcggt agagaaaggc ctggttgatg aaatcaacac cagtgatgaa
    12001 gttattctta gcctgatgga aggccgtgaa gtggttaatg tacgctatat gcagcgtaaa
    12061 cgactcattg accgattcac cggcagcgcg gcagagagcg ccgatcgatt gttgctacgc
    12121 tggtggcagc ggggtcaaaa gccattgatg taaaagacaa acgcgaggct aagacctcgc
    12181 gttttgcttt aatcaaccag atgatatttt tctgaaagca catgggccag gtgtttgaac
    12241 atattaaaca ccgcggtgct tttggctgtt ggcaatcctt gttcatctaa aaagtagtcg
    12301 ccggtaaata ccagcacgcc attacgctgc gtgacgccgg tggcttcaat ccctgcgagc
    12361 gtatcctcat gctcacgaat gattttgttg gcctctttca acagcgtttc gcggtcgatg
    12421 ggttgtgtct ctttgttcat tatttactcc ttaaacaagg acattagtct acgccaggca
    12481 tggcttgcag acaaatatac cacgctggtg gcaagagcgc cttactggta actttggatt
    12541 ttgcatgcta ataaagttgc gtatcggatt ttatcaggta cagtgtgacg ctttcgtcaa
    12601 tctggcaata gatttgcttg acattcgacc aaaattccgt cgtgctatag cgcctgcagg
    12661 ccaagacctg ttaactcagt cacctgaatt ttcgtgaaca gagtcacgac aaggggttga
    12721 tatccgcaga gaacgagtcc atatcggtaa ctcgttgcca gtggaaggtt tatcaacgtg
    12781 cgacgcattc ctggaagaat caaattaggt aaggtgaata tgggtaaagc tcttgtcatc
    12841 gttgagtccc cggcaaaagc caaaacgatc aacaagtatc tgggtagtga ctacgtggtg
    12901 aaatccagcg tcggtcacat ccgcgatttg ccgaccagtg gctcagctgc caaaaagagt
    12961 gccgactcta cctccaccaa gacggctaaa aagcctaaaa aggatgaacg tggcgctctc
    13021 gtcaaccgta tgggggttga cccgtggcac aattgggagg cgcactatga agtgttgcct
    13081 ggtaaagaga aggtcgtctc tgaactgaaa caactggctg aaaaagccga ccacatctat
    13141 ctcgcaaccg accttgaccg cgaaggggaa gccattgcat ggcacctgcg ggaagtgatt
    13201 ggtggtgatg atgcgcgcta tagccgagtg gtgtttaacg aaattactaa aaacgcgatc
    13261 cgccaggcat ttaacaaacc gggtgagctg aatattgatc gtgttaatgc ccagcaggcg
    13321 cgtcgcttta tggaccgcgt ggttgggtat atggtttcgc cgctgctatg gaaaaagatc
    13381 gctcgtggtc tgtctgccgg tcgtgtgcag tcggtggcgg ttcgcctggt ggtcgagcgt
    13441 gagcgtgaaa ttaaagcgtt cgtgccggaa gagttctggg aagtcgatgc cagcacgacc
    13501 acgccatctg gtgaagcgtt ggcgttgcag gtgactcatc agaacgacaa accgttccgt
    13561 ccggtcaaca aagaacaaac tcaggctgcg gtaagtctgc tggaaaaagc gcgctacagc
    13621 gtgctggaac gtgaagacaa accgacaacc agtaaacctg gcgctccttt tattacctct
    13681 acgctgcaac aagctgccag cacccgtctt ggatttggcg tgaaaaaaac catgatgatg
    13741 gcgcagcgtt tgtatgaagc aggctatatc acttacatgc gtaccgactc cactaacctg
    13801 agtcaggacg cggtaaatat ggttcgcggt tatatcagcg ataattttgg taagaaatat
    13861 ctgccggaaa gtccgaatca gtacgccagc aaagaaaact cacaggaagc gcacgaagcg
    13921 attcgtcctt ctgacgtcaa tgtgatggcg gaatcgctga aggatatgga agcagatgcg
    13981 cagaaactgt accagttaat ctggcgtcag ttcgtagcct gccagatgac cccagcgaaa
    14041 tatgactcca cgacgctgac cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt
    14101 attttgcgtt ttgatggctg gacaaaagtg atgcctgcgt tgcgtaaagg cgatgaagat
    14161 cgcatcttac cagcagttaa taaaggcgat gctctgacgc tcgttgaact tacaccagcc
    14221 cagcacttta ccaagccgcc agcccgtttc agtgaagcat cgctggttaa agagctggaa
    14281 aaacgtggta tcggtcgtcc gtctacctat gcgtcgatca tttcgaccat tcaggatcgt
    14341 ggctacgtgc gagtagaaaa tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc
    14401 gatcgccttg aagaaaattt ccgcgagtta atgaactacg actttaccgc gcagatggaa
    14461 aacagcctcg accaggtggc aaatcacgaa gcagagtgga aagctgtact ggatcacttc
    14521 ttctcggatt tcacccagca gttagataaa gctgaaaaag atccggaaga gggtggtatg
    14581 cgcccgaacc agatggttct gaccagcatt gactgcccga cttgtggtcg caaaatgggg
    14641 attcgcacag cgagcaccgg ggtattcctt ggctgttctg gctatgcgct gccgccgaaa
    14701 gagcgttgca aaaccaccat taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa
    14761 ggcgaagatg ctgaaaccaa cgcgctgcgc gcaaaacgtc gttgcccgaa atgcggcacg
    14821 gcgatggaca gctatctcat cgatccgaaa cgtaagttgc atgtctgtgg taataaccca
    14881 acctgcgacg gttacgagat cgaagagggc gaattccgca ttaaaggtta tgacggcccg
    14941 atcgttgagt gtgaaaaatg tggctctgaa atgcacctga aaatggggcg tttcggtaaa
    15001 tacatggcct gcaccaacga agagtgtaaa aacacacgta agattttacg taacggcgaa
    15061 gtggcaccac cgaaagaaga tccggtgcca ttacctgagc tgccgtgcga aaaatcagat
    15121 gcttatttcg tgctgcgtga cggtgctgcc ggtgtgttcc tggctgccaa cactttcccg
    15181 aaatcgcgtg aaacgcgtgc gccactggtg gaagagcttt atcgcttccg cgaccgtctg
    15241 ccggaaaaac tgcgttatct ggccgatgcg ccacagcagg atccggaagg taataagacc
    15301 atggttcgct ttagccgtaa aaccaaacag caatatgtct cttcggaaaa agacggaaag
    15361 gcgactggct ggtcagcatt ttatgttgat ggcaaatggg ttgaaggaaa aaaataacct
    15421 ttaattctgt caggttttta taaacaaagg gtcgcgaaag cggccctttt ttattgcata
    15481 ttattt
//
LOCUS       DAZFAH010000104        15116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFAH010000104 DAZFAH010000000
VERSION     DAZFAH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.104"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..602
                     /gene="yceJ"
                     /locus_tag="ACHJWB_003022"
     CDS             36..602
                     /gene="yceJ"
                     /locus_tag="ACHJWB_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4314611.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLLSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            606..1181
                     /gene="yceI"
                     /locus_tag="ACHJWB_003023"
     CDS             606..1181
                     /gene="yceI"
                     /locus_tag="ACHJWB_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4314612.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1223..2275)
                     /gene="trhO"
                     /locus_tag="ACHJWB_003024"
     CDS             complement(1223..2275)
                     /gene="trhO"
                     /locus_tag="ACHJWB_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4314613.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2500..3420
                     /gene="lpxL"
                     /locus_tag="ACHJWB_003025"
     CDS             2500..3420
                     /gene="lpxL"
                     /locus_tag="ACHJWB_003025"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4314614.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3592..4818
                     /gene="mdtG"
                     /locus_tag="ACHJWB_003026"
     CDS             3592..4818
                     /gene="mdtG"
                     /locus_tag="ACHJWB_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO4314615.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4901..5275
                     /gene="msyB"
                     /locus_tag="ACHJWB_003027"
     CDS             4901..5275
                     /gene="msyB"
                     /locus_tag="ACHJWB_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4314616.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5276..5503)
                     /gene="yceK"
                     /locus_tag="ACHJWB_003028"
     CDS             complement(5276..5503)
                     /gene="yceK"
                     /locus_tag="ACHJWB_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4314617.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5676..8219)
                     /gene="mdoH"
                     /locus_tag="ACHJWB_003029"
     CDS             complement(5676..8219)
                     /gene="mdoH"
                     /locus_tag="ACHJWB_003029"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4314618.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8212..9747)
                     /gene="mdoG"
                     /locus_tag="ACHJWB_003030"
     CDS             complement(8212..9747)
                     /gene="mdoG"
                     /locus_tag="ACHJWB_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4314619.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNAYQTLSETWSYQLP
                     ANE"
     gene            10141..11298
                     /gene="mdoC"
                     /locus_tag="ACHJWB_003031"
     CDS             10141..11298
                     /gene="mdoC"
                     /locus_tag="ACHJWB_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4314620.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGVLAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11306..12787)
                     /gene="clsC"
                     /locus_tag="ACHJWB_003032"
     CDS             complement(11306..12787)
                     /gene="clsC"
                     /locus_tag="ACHJWB_003032"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4314621.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12729..13262)
                     /gene="ymdB"
                     /locus_tag="ACHJWB_003033"
     CDS             complement(12729..13262)
                     /gene="ymdB"
                     /locus_tag="ACHJWB_003033"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4314622.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13357..13668)
                     /gene="ymdA"
                     /locus_tag="ACHJWB_003034"
     CDS             complement(13357..13668)
                     /gene="ymdA"
                     /locus_tag="ACHJWB_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO4314623.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            complement(13789..14121)
                     /gene="csgC"
                     /locus_tag="ACHJWB_003035"
     CDS             complement(13789..14121)
                     /gene="csgC"
                     /locus_tag="ACHJWB_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4314624.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14181..14636)
                     /gene="csgA"
                     /locus_tag="ACHJWB_003036"
     CDS             complement(14181..14636)
                     /gene="csgA"
                     /locus_tag="ACHJWB_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4314625.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14677..>15116)
                     /gene="csgB"
                     /locus_tag="ACHJWB_003037"
     CDS             complement(14677..>15116)
                     /gene="csgB"
                     /locus_tag="ACHJWB_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4314626.1"
                     /translation="FMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAIIG
                     QAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQGA
                     YGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3759 a         3894 c         3618 g         3845 t
ORIGIN      
        1 tttctttctc tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac
       61 gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt
      121 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac
      181 ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt
      241 ggcgtgtcat atctccaccg ccgggtccgc tgctgagtta ttcgcccatg actcgtcttg
      301 ccgcaaaggc tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct
      361 atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
      421 cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg
      481 cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca
      541 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
      601 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt
      661 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc
      721 gcatccagca ccttggctat agctggttat acggcacctt taaagatttc gacggtactt
      781 ttacctttga cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca
      841 gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata
      901 ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac
      961 tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga
     1021 aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag
     1081 gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag
     1141 tggatctgat tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat
     1201 ggcacgcatc cggcaatgat atttattccg ttggatcagg aatgcccagt gttgtattca
     1261 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
     1321 gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
     1381 atttttccgc acatactgga cactgaataa acagcaggtg gcagccatca tttttacagt
     1441 tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc tcatcagata
     1501 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc
     1561 cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt
     1621 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
     1681 acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
     1741 cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat
     1801 agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg
     1861 ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg
     1921 caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca
     1981 acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa
     2041 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
     2101 acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct
     2161 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcag
     2221 ccaacatttt ggcttttagc gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
     2281 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
     2341 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg
     2401 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
     2461 acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc
     2521 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
     2581 ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg
     2641 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
     2701 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
     2761 atgggcctga tggaaaccgg catggcgtgg ttctggccgg accgccgaat cgcccgctgg
     2821 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
     2881 ttagttggca tccattttct gacactggaa ctgggcgcgc ggcagtttgg tatgcaggaa
     2941 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
     3001 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
     3061 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
     3121 agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg
     3181 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
     3241 aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa
     3301 actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
     3361 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
     3421 atctcccctg ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat
     3481 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
     3541 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
     3601 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
     3661 tttcttaccg gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag
     3721 cttggcgtta ctggtcactc cgccctgaat atgtggtccg gtattgtctt cagcattaca
     3781 tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga
     3841 aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatgggactg
     3901 gcacaaaata tctggcagtt tttgatccta cgggcgcttc ttgggttact tggcggattt
     3961 gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg
     4021 ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc
     4081 ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
     4141 ctctgctttt tcgtcaccct gttctgcatc agagaaaaat tccagccggt cagcaaaaaa
     4201 gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
     4261 ctgtttgtca ccacgttaat catccaggtg gcgacaggct caattgcccc cattctgacg
     4321 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
     4381 gcctcggtgc caggcgtggc ggctctgcta agcgcaccac gactcggcaa acttggcgat
     4441 cgaatcggac ccgaaaagat cctgattaca gcgctgatct tttctgtact gctgttgatc
     4501 ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt gctcggtgcc
     4561 gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc gagcaaccag
     4621 atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa cgttaccgga
     4681 ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcacc
     4741 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata
     4801 ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc
     4861 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
     4921 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
     4981 cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
     5041 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct
     5101 tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag agatacgcca
     5161 ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc agcttgagcc
     5221 accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac gttaatcact
     5281 catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc agagagaatg
     5341 gcagatcaag gatcgtgaca taacgccagg cggagtcacg cacatcccat tgcacaccgg
     5401 gataatattg gttgccatgc ccctgccccg gtatagtgcg actaataatg ctgccacagc
     5461 cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac ctctttcgta
     5521 aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc ggatgcggcg
     5581 tgaacgcctt atccgaccca caaaatcttg caaaatcaat aaattgcagg aacgatgtag
     5641 gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc atccggttta
     5701 cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga agaatatctc
     5761 tccggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc gcttagcagc
     5821 accaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg ttcaacgtgg
     5881 cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc cattgcggtt
     5941 gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcatc aagggagcgt
     6001 tgacgattca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg cgaatactct
     6061 tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc acggctggaa
     6121 atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag ccagaacagg
     6181 aaacgcagat ccaaccacgc cataccaaca gcccacacta accctaacag cagctgtgag
     6241 ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg cggtgaattc
     6301 cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa cagcatgcgt
     6361 accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt aacgcgccag
     6421 aagccgccgt attctttcgt tcctttgcac cagataagca aaatgctcaa taacttcggc
     6481 aggaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg acgccactgc
     6541 ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt caacgcatgt
     6601 actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc ggagagataa
     6661 gacatcacgc ccgtcaggaa caccgcacgg tgaaccgggt gcataccctt caccaggaac
     6721 agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag ctcatcaagc
     6781 aagttaggcg gcaattcttc ataagaaccc gggagatcgt aagcaatcca gaccccccaa
     6841 cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg acaggattga accggcaaag
     6901 gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt cacgcggata
     6961 atcgcgttat gtccccagta gtgcgactcg ccaagttgcc agaagtgcaa accggctgta
     7021 aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gcgcatacag cgtatccata
     7081 ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc catcaagcgc
     7141 accaggccgc ataaacaatc accggtcatt accgagtcag catccagcac caccatgtag
     7201 ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt acgcttcacg
     7261 cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc gataagctcc
     7321 atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact aagaatgtag
     7381 acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc acgcaggcca
     7441 gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt gcgatgctcc
     7501 gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc gcgaccaata
     7561 agcagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca acagaacagt
     7621 accgcaaaga ggatcagaat accggtttgc agcatataag gcagaagctg cataaaggaa
     7681 acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc ctgataagga
     7741 agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag gatcaacaga
     7801 atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg ctcttctttg
     7861 gtcaaacgag ccagatagcg cggcgtgaca tcgcgtccac gcaggcgatc ccagaaacgg
     7921 cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc ttctggcatc
     7981 gccttcagct gatcgcgccc ttcgtcgtct ttaattaact gtccatcagc aagtgaatct
     8041 ggccaggctt gttccagacg cgcctttacc gagccttgcg gggaatcatc ctcccgcgcc
     8101 caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt cttcggcaat
     8161 gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt cttattcatt
     8221 ggcaggtaac tggtagctcc aggtttcact caacgtctga taggcattca ccagcgcagc
     8281 acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca gacgccagcc
     8341 tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat caccaatgct
     8401 ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc cggtaaaatc
     8461 gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct tcacatcccc
     8521 cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt tgtcttcatc
     8581 acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg gcagctgatc
     8641 cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa tttccaccag
     8701 ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg gacggagatc
     8761 gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca acagaccaaa
     8821 gccttgcggg ttttccatgg agaagctgct gaccgctaaa tgtttcgggt tattcaacgg
     8881 acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt cgtgcaactc
     8941 cggacgatag ttatttgcag gcgacggttg gttcggccca aacaggaaca tactggttaa
     9001 cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact gcacatccac
     9061 aaccgtgtca cgccctggca taaccacgaa tttgtaagca cctgtcgcgc gcggcgagtc
     9121 aagcaatgca tagatggtta aacgtttatc agtcggtttt ggacgctcga tccagaactc
     9181 tttgaagcgc ggaaattctt cacccgatgg caaggcggta tcaattgcca ggccgcgggc
     9241 agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc cgagcatgct
     9301 gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa aaccggcaaa
     9361 gccaaggtct tttaccgtat ctttgtcatg ctgaacatcg ccgaaagtga aataatccgg
     9421 gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga ccggggtatc
     9481 gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat tgttccagta
     9541 cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat cgcggaaaac
     9601 ggagggcaag ttgcttttgg gcgtctcgta gcctttcccg gctaaagatt gagcttgctt
     9661 tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta acattactgc
     9721 agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta gtaagcactt
     9781 ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc cattccgaca
     9841 acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga ttgcaaccgt
     9901 tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg gtttgccagc
     9961 aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta gcgatgatgc
    10021 tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtca ctatggcaac
    10081 aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag ttaaccatat
    10141 atgaacccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc ctggctgatg
    10201 ttgttaggga taccttttca tatttcttta atctattcga gccatacatg gcatgtgaat
    10261 agcgccgaac catcattgtg gctgaccctt tttaatgact tcatccactc gttccgcatg
    10321 caggtatttt tcgttatatc cggctacttt tcctacatgc tttttttacg ctatcccttg
    10381 aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac agccatcccc
    10441 ctactgacat taccgcaatt tattatgctg caatatgtca aaggaaaagc ggaaagttgg
    10501 cctgggctgt cattgtatga caaatataat acgttggcct gggaattaat atcacacctg
    10561 tggtttttac tggtgttagt agtcatgacg acgctgtgcg tatggatatt taagcgcatt
    10621 agaaataatt tagaaaattc tgataaaacg aataaaaaat tctcgatggt aaaactatcg
    10681 gtgatttttt tatgcctcgg catcggttat gcggtaataa gaagaacgat ttttattgtg
    10741 tatccgccca ttctgagtaa tggcatgttc aattttattg tcatgcaaac gctgttttat
    10801 ttgccgttct ttatcctcgg cgtactggct ttcattttcc ctcatcttaa agccttgttt
    10861 accacgccgt ctcgtggctg tacccttgca gcagcattgg cgtttgtcgc ttatttactc
    10921 aaccagcgct atggcagtgg cgatgcctgg atgtacgaaa ccgagtcggt gatcaccatg
    10981 gtcctcggtc tgtggatggt gaatgtggtc ttctcctttg gccaccgttt gcttaacttc
    11041 cagtcagcgc gggtgactta cttcgttaac gcatcgctat ttatctatct ggttcaccac
    11101 ccgttaacgc tgtttttcgg cgcatacatt acaccgcaca tcacctccaa ctggcttggt
    11161 tttctctgtg gcctgatatt tgtagtaggg attgcgataa ttctgtatga aattcatttg
    11221 cgcatcccgt tactgaagtt tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat
    11281 aaagcaccag cccgttaagc cacatttaca ataaccattc cacgggcaat atcgacgcca
    11341 gtctgaccat aacccgcttc cagaaactgg tggcgggttc ttttttgaga ataatctctt
    11401 tctttgcatg acgatcaacc cagttgatcc gtccccacct gtccagacgg agctgccagg
    11461 ccgcatcata ctggctctga ataaagcgtt tatcaattaa ctgtgccagc gtttcgctct
    11521 ctatcacgaa gcccatttca gtattgagca atgttgaacg cggatcgaaa ttgaaagagc
    11581 cgataaacac cgttttacca tcgatgctaa aggttttagc atgcaggctg gctccggaat
    11641 taccggttat gccgcgatcg tgtaacgtac tactttgttc acgcgtcggc ttgagttcat
    11701 ataattccac gccatagcgg agcaattttt tgcgccagcg cgcgtatccg gcatggacga
    11761 cagcaacatc gttagcggca agagaattgg ttaggatcgc aatctttacc ccttttctca
    11821 ccatccgtaa gagttgcgcc acacctgcgc gtgtcggtac aaaataggaa gagataatat
    11881 cgatgcgttc actgggtgag cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc
    11941 gttttgcctt gccctcccct ttcgccggat catcacttaa taaacgtgtc ttcgcccaga
    12001 taagcggcaa tgttccatca accagatgat ttataaatgg actggattcc attttgcgta
    12061 aataacgatg cgtcatggca tcgttatgcc aggaggcggg taactcgatg cgatccgcca
    12121 tttcaccctc cgggacatcc agcacctgct gtaagggtga aaccgatttg caataccagt
    12181 agcgggcgaa atcatcggca acgtcctcta ccacgggtcc tattgccatg acatctaaat
    12241 ccgaaaaaag tggctcctcc cctgctccaa aataggcatc accaatattt cgtcctccca
    12301 ccagggtcac cacgccatcg acagtgaaac ttttattgtg catacggcga ttaagacggg
    12361 aaaagtcggt gatataacca agcggacgca gcaagcgaaa cgagaaagga ttaaaaagcc
    12421 ggacttcaat gcgtggatga ctgtcaagca agcgtaaaat gtcgtcaagt ccgggcgtat
    12481 tgttgtcgtc cagcaacaaa cggacgcgaa cgccacgctt tgctgcggct aacagggcgg
    12541 aaaacagtaa ccgacccgac atatcgtcct gccagatgta atactgaaca tcgagcgtat
    12601 gctctgccat ttcggccaga cgataccggg cggcaaacgc atccagactt ttctccagag
    12661 gaaaaaggcc acactgaccg ggatgttgcg aacacagtgg cagcaccgcg ctcgccagcc
    12721 ggggcaaatc attcatctcc ttgttgggta aggagtcttt cgtagaggtg ggcgttttct
    12781 tcatcataac agacaaagta tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa
    12841 acggttttta ctgcgatttc agccgctgcc gcacgagggt aaccataaac cccagtactg
    12901 attgcaggaa aagccactga cgtatagctg tttgccgcca ccagtcgtag gctattgaga
    12961 taggcatcct gcaaaagctg gtcttcgttt tgttcaccac cacgccagac tggcccgacg
    13021 gtgtgcacta cggctttagc gggaagatcg cctgcaagcg taataacggc atgccccgta
    13081 gggcaatcgc cctgctgttg cctgactttt aaacaggcat ccagtagggc cggacccgct
    13141 gcgcgatgaa tggccccatc gacgccgcca cctcccatta atgaaggatt agccgcattc
    13201 acaatcacat caacggccag tttggtaata tcaccctgca caacatgaat acgcgttttc
    13261 ataacgcctc cttacacctt cttgtttctt aagtgtacag caggcttttt ggttaaatcg
    13321 catttttcta ctcagcaacg tgagttgttc agaaacttag ttatattcga tgttcattac
    13381 agccagcttt ttctgtttat caatatactg cacttttact gaggctactt gcgcgttttt
    13441 gacattgccg ctcattaacg ctttgctgga ataggttttt cccggaacag aaccattctg
    13501 tgggcagttc atttctacgg gtgatgactg ggtgctgacg tcacatgccg gttccacaat
    13561 ttggccataa aaatggatca cgcccccttg cgcggtgctt cctgcgatgg caataaaagg
    13621 aaaaaacaaa ctgccgagca taagagagtt taaaaaaggt ctgaacattt attctatttc
    13681 ctcaatgatt agtcatcctt gagggttgtg ttatccatac tttcaggttg gctaattcgc
    13741 agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt aagatttttc
    13801 tgaagagggc ggccattgtt gtgataaatg aagtgactgt ccatcagaaa cagtaacaac
    13861 tattttcacc cgatcgtccg gggaaatatt taaactcaac ttcgtcaaag caatgggttg
    13921 attagcaggc aatgaaaggg tcttttcttg cttcgtctga ctttgccctg aactgccttc
    13981 gcgcagggac aatatttgta ctctgcacag acaagattga gtaagagtga cttcaggaat
    14041 aatggtatac acatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc
    14101 aaggagtaat aacgcattca tattcttctc ccgaaaaaaa aacagggctt gcgccctgtt
    14161 tctgtaatac aaatgatgta ttagtactga tgagcggtcg cgttgttacc aaagccaacc
    14221 tgagtcacgt tgacggagga gttagatgca gtctggtcaa ctgcagcacc gttgccacca
    14281 ccgaactgtt taaccgtcat ttcagaattt ttgccgttcc actgatcaag agtagcgctg
    14341 ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ccgagccctg accaacatct
    14401 gcaccattac cgccgccatg ctgggtaata gtcaagtcag agttacgggc atcagtttgc
    14461 agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga atttgggccg
    14521 ctattattac cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc
    14581 agagcgctac cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa
    14641 aacccccatc ggattgattt aaaagtcgaa tggaaattaa cgttgtgtca cgcgaatagc
    14701 catttgcgac tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt
    14761 tgctttatta ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga
    14821 aatactggca tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt
    14881 ctggtcaatc tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga
    14941 gcctccctgc cgtaactgag cactattatt cgtcccagct tgaccaatta tggctgcctg
    15001 attaaatgaa gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc
    15061 ataacctgct gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaata
//
LOCUS       DAZFAH010000105        15051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFAH010000105 DAZFAH010000000
VERSION     DAZFAH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.105"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..920
                     /gene="ybeQ"
                     /locus_tag="ACHJWB_003038"
     CDS             <1..920
                     /gene="ybeQ"
                     /locus_tag="ACHJWB_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO4314627.1"
                     /translation="EKGDCEAQYIVGFYYNRDSAIDSPDDEKAFYWLKLAAEQGHCEA
                     QYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASNALGWILDRGEDPNYKEAVVW
                     YQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYKQAALQGHSDAQNNLADLYED
                     GKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGVDQDYKQAMYWYLKAAAQG
                     SVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATAWYNLAIMYHYGEGRPVDL
                     RQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(990..1472)
                     /gene="ybeL"
                     /locus_tag="ACHJWB_003039"
     CDS             complement(990..1472)
                     /gene="ybeL"
                     /locus_tag="ACHJWB_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4314628.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1707..4289
                     /gene="leuS"
                     /locus_tag="ACHJWB_003040"
     CDS             1707..4289
                     /gene="leuS"
                     /locus_tag="ACHJWB_003040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4314629.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4304..4885
                     /gene="lptE"
                     /locus_tag="ACHJWB_003041"
     CDS             4304..4885
                     /gene="lptE"
                     /locus_tag="ACHJWB_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4314630.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4885..5916
                     /gene="holA"
                     /locus_tag="ACHJWB_003042"
     CDS             4885..5916
                     /gene="holA"
                     /locus_tag="ACHJWB_003042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4314631.1"
                     /translation="MIRLYPEQLRAQLNEGLRTAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5918..6559
                     /gene="nadD"
                     /locus_tag="ACHJWB_003043"
     CDS             5918..6559
                     /gene="nadD"
                     /locus_tag="ACHJWB_003043"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4314632.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6583..7194
                     /gene="cobC"
                     /locus_tag="ACHJWB_003044"
     CDS             6583..7194
                     /gene="cobC"
                     /locus_tag="ACHJWB_003044"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4314633.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7454..7771
                     /gene="rsfS"
                     /locus_tag="ACHJWB_003045"
     CDS             7454..7771
                     /gene="rsfS"
                     /locus_tag="ACHJWB_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4314634.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7775..8242
                     /gene="rlmH"
                     /locus_tag="ACHJWB_003046"
     CDS             7775..8242
                     /gene="rlmH"
                     /locus_tag="ACHJWB_003046"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4314635.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8273..10174
                     /gene="mrdA"
                     /locus_tag="ACHJWB_003047"
     CDS             8273..10174
                     /gene="mrdA"
                     /locus_tag="ACHJWB_003047"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4314636.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10177..11289
                     /gene="mrdB"
                     /locus_tag="ACHJWB_003048"
                     /gene_synonym="rodA"
     CDS             10177..11289
                     /gene="mrdB"
                     /locus_tag="ACHJWB_003048"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4314637.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11300..12388
                     /gene="rlpA"
                     /locus_tag="ACHJWB_003049"
     CDS             11300..12388
                     /gene="rlpA"
                     /locus_tag="ACHJWB_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4314638.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVHGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12527..13738
                     /gene="dacA"
                     /locus_tag="ACHJWB_003050"
     CDS             12527..13738
                     /gene="dacA"
                     /locus_tag="ACHJWB_003050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4314639.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13849..14112
                     /gene="ybeD"
                     /locus_tag="ACHJWB_003051"
     CDS             13849..14112
                     /gene="ybeD"
                     /locus_tag="ACHJWB_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4314640.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14213..14854
                     /gene="lipB"
                     /locus_tag="ACHJWB_003052"
     CDS             14213..14854
                     /gene="lipB"
                     /locus_tag="ACHJWB_003052"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4314641.1"
                     /translation="MYQDKILVRQLGLQPYEPITQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWQPEATTNNIAPRLLQNILALLNNPDFEYITA"
BASE COUNT         3703 a         3830 c         3940 g         3578 t
ORIGIN      
        1 ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt ttattataat cgcgatagcg
       61 caattgattc tccggacgac gaaaaagcct tttactggct gaagctggcc gccgagcaag
      121 gtcattgtga agcacaatat tccttaggac aaaagtatac tgaggataaa agccgtcata
      181 aagataatga gcatgccatc ttttggctga aaaaagctgc cctacaaggc catactttcg
      241 cttccaacgc ccttggctgg atactggatc gtggagaaga ccccaattat aaagaagcgg
      301 ttgtctggta tcagatagcc gcggagagcg gaatgtctta tgcgcaaaat aatcttgggt
      361 ggatgtacag aaatggcaac ggagtcgcaa aagactatgc gctggcattt ttctggtaca
      421 aacaagctgc attacaaggc catagtgacg cgcaaaacaa tctggccgat ctttatgaag
      481 acggaaaagg cgttgctcaa aacgagacac tcgccgcatt ctggtatttg aaaagcgcac
      541 agcagggtaa tcggcatgct cagtttcaaa ttgcatggga ttataacgct ggcgaagggg
      601 tggaccagga ctataagcaa gcgatgtact ggtatctgaa ggctgccgct caggggagcg
      661 tcggcgctta cgtcaacatc ggttatatgt ataaacacgg acaaggcgtt gagaaagatt
      721 atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg caatgacgcc actgcctggt
      781 ataacctggc cattatgtat cactacggag aaggaagacc tgtcgatctc cgacaggctc
      841 tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga tgtcagtcaa gaaattcgtg
      901 agattgaaga tttactgtag agataaaaac aagggccggg gaatttcatt cggcccatat
      961 tctctttatt gctgtgcgaa atcagactat tacggctcaa acgggcgtct ctggaactgg
     1021 tcatgaccac atttcgggca tagcgtcagc acttccggtg tgtagatcgg gagatggaag
     1081 tgacatttct cgcagaccag atttcccagc ccgaccactt ctccgctgtg ataaacccca
     1141 tgatgattga ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatgtct
     1201 gccagctcct gccacaagct ttctttaatc acccgcataa agacgctgtc agattcttct
     1261 ttcaggctct cttcatagct catggcgaac tcttccaggt cacgtctgac agctcgcgtc
     1321 agctcatcga cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgt
     1381 tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc gttcgctcag tgacgcaacc
     1441 agttcacggt aatattgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac
     1501 tataataata gacgttcttt gttccttgct ttgctaatac gcccacaaac tctgtaaatt
     1561 aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc
     1621 ggacgtttgg gctatgctat gcggatctga aaaaccacct caacgctaca tttgtagccg
     1681 tattgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat
     1741 ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa gacgagagca
     1801 aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc
     1861 acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg ctgggcaaaa
     1921 acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc gcggcggtga
     1981 aaaacaacac cgctccggca ccgtggacgt acgacaacat cgcgtatatg aaaaaccagc
     2041 tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt acgccggaat
     2101 actaccgttg ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaga
     2161 agacttctgc ggtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta
     2221 tcgacggctg ctgctggcgc tgcgatacca aagttgaacg taaagagatc ccgcagtggt
     2281 ttatcaaaat cactgcttac gctgacgagc tgctcaacga tctggataaa ctggatcact
     2341 ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa ggcgtggaga
     2401 tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt ttacactacc cgcccggaca
     2461 cctttatggg ttgtacctac ctggcggtag ctgcgggtca tccgctggcg cagaaagcgg
     2521 cggaaaataa tcctgaactg gcggccttta ttgacgaatg ccgtaacacc aaagttgccg
     2581 aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa gctgttcacc
     2641 cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gagtacggca
     2701 cgggcgcagt tatggcggtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat
     2761 acggcctgaa catcaaacca gttatcctgg ctgctgacgg ctctgagcca gatctttctc
     2821 agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg cgaattcaac ggtcttgacc
     2881 atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta
     2941 aagtgaacta ccgcctgcgc gactggggtg tttctcgtca gcgttactgg ggcgcgccga
     3001 ttccgatggt gacgctggaa gacggtaccg taatgccgac cccggacgac cagctgccgg
     3061 tgatcctgcc ggaagatgtg gtgatggacg gcattaccag cccgattaaa gcagatccgg
     3121 agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac actttcgaca
     3181 cctttatgga gtcctcctgg tactatgcgc gctacacttg cccagagtac aaagaaggta
     3241 tgctggattc cgaagcggct aactactggc tgccggtgga tatctacatt ggtggtatcg
     3301 aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag
     3361 gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg gtgctggcag
     3421 atgccttcta ctatgttggc gaaaacggcg aacgtaactg ggtttccccg gttgatgcta
     3481 tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca ggccatgaac
     3541 tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg
     3601 tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg
     3661 ctgatatgac tctcgaatgg caggaatccg gtgtggaagg ggctaaccgc ttcctgaaac
     3721 gtgtctggaa actggtttac gagcacacag caaaaggtga tgttgcggca ctgaacgttg
     3781 atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt gcataaaacg atcgctaaag
     3841 tgaccgatga tatcggccgt cgtcagacct tcaacaccgc aattgcggcg attatggagc
     3901 tgatgaacaa actggcgaaa gcaccaaccg atggcgagca ggatcgcgct ctgatgcagg
     3961 aagcgctgct ggccgttgtc cgtatgctta acccgttcac cccgcacatc tgcttcacgc
     4021 tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg
     4081 aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg
     4141 ccaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg
     4201 aacatctggt agcaaaatat cttgatggcg ttaccgtgcg taaagtgatt tacgtaccag
     4261 gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac
     4321 attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgatac
     4381 cacgcaggtt ccttccacta tgaaggtcat gatcctggac tcaggcgatc cgaacgggcc
     4441 attaagccgt gcggtgcgta accagttacg tctgaatggt gtcgagttgc ttgataaaga
     4501 aaccacgcgt aaggacgttc catccctgcg tttgggtaaa gtgagcatcg cgaaagatac
     4561 cgcatcggta ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtgaatgc
     4621 gaccgtgttg atccccggcc gtgatatcta cccgattagc gccaaagtct tccgttcgtt
     4681 ctttgataac ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga
     4741 gatgtacgac cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga
     4801 tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg
     4861 cgtctccacc acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag
     4921 ctcaatgaag ggctgcgcac ggcgtatctt ttacttggta acgatcctct gttattgcag
     4981 gaaagccagg acgctgttcg tcaggtagct gcggcacaag gattcgaaga acaccacact
     5041 ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca ggctatgagt
     5101 ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc gaatgcggcg
     5161 atcaatgaac aacttctcac actcaccgga cttctgcatg acgacctact gttgatcgtc
     5221 cgcggtaata aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat
     5281 cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg ctgggttgct
     5341 gcgcgcgcaa aacagctcaa cttagaactg gatgacgcgg cgaatcaggt gctctgctac
     5401 tgttatgaag gtaacctgct ggcgctggct caggcactgg agcgtttatc gctgctctgg
     5461 ccagacggca aactgacatt accgcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc
     5521 accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc gttgcatatt
     5581 cttcagcaac tgcgtctgga aggcagcgag ccggttattt tgttgcgcac attacaacgt
     5641 gaactgttgt tactggttaa cctgaaacgc cagtctgccc atacgccact gcgtgcgttg
     5701 tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc
     5761 ttaagccagc cgcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc
     5821 aaacaagatt acggtcagtc agtgtgggca gagctggaag ggttatctct tctgttgtgc
     5881 cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt
     5941 tggcggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa cgctggcgaa
     6001 tttgattggt ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca
     6061 gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc
     6121 attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac
     6181 actgaaagag tggcggcagg aacaagggcc ggacgtgccg ctggcgttta ttattggtca
     6241 ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca
     6301 tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc aataccagca
     6361 atggctggaa gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa
     6421 aatttatctg gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt
     6481 gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg gtactgactt acattaacca
     6541 acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt
     6601 cgtcatggcg aaacgcaagc gaatgtcgat ggtctgtaca gcggtcatgc gcccacaccc
     6661 ctgaccgcac gcggtattga gcaagcgcaa aatctgcata cactgctaga tgatgtttcc
     6721 tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg acgggttctc
     6781 agtgaccgcc agctccccgt tcatatcatc cctgaactca acgaaatgtt ttttggcgac
     6841 tgggagatgc gacatcatcg cgacctcatg caagaagatg ccgaaaacta tagcgcgtgg
     6901 tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc attttcgcaa
     6961 cgtgtggaaa gatttatcgc aaggcttagt gaatatcagc actatcagaa tattttaatc
     7021 gtcagccatc agggcgtact gagtctgtta attgctcgtc taattggcat gcctgccgaa
     7081 tctatgtggc attttcgcgt tgaccagggg tgttggagcg ccattgatat caaccaaaaa
     7141 ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc
     7201 tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc
     7261 gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat
     7321 gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt
     7381 gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac
     7441 ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga
     7501 cctcaaaggt caggacatca tcgccttaga cgttcagggc aaatccagca tcaccgactg
     7561 catgatcatc tgtacgggta cgtccagccg tcatgttatg tccattgctg accacgttgt
     7621 gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga
     7681 ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg
     7741 cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg
     7801 aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa
     7861 agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat
     7921 caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat
     7981 tgtcaccctc gatattccag gcaagccctg ggatacaccg cagttagccg ctgagctgga
     8041 acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc
     8101 gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg tcggcgctta ccctccccca
     8161 tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa
     8221 ccatccttat caccgtgagt gataagggag ctttgagtag aaaacgcagc ggatgaaact
     8281 acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt
     8341 cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca
     8401 aattgttcgc tttaccgact accagactcg ctctaatgaa aaccgcatta agctggtgcc
     8461 tatcgcgccc agccgcggca ttatctacga ccgtaatggt atccctctgg ccctcaaccg
     8521 cactatctac cagatagaaa tgatgccaga gaaagtcgat aacgtgcagc aaacgctgga
     8581 cgctttgcgc agcgtggttg atctgaccga tgacgatatt gctgcattcc gaaaagagcg
     8641 cgcacgttca caccgtttca cctctattcc ggtgaaaact aacctgaccg aagtacaagt
     8701 agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc gaagttaaag gctataaacg
     8761 tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa
     8821 cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca
     8881 tgatatcggt aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagaccgg
     8941 ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc
     9001 accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc aatatattga
     9061 aacgctgctg gcgggtagcc gcgcagctgt ggttgtcacc gatccgcgta caggtggggt
     9121 gctggcgctg gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag
     9181 caaagattat tccgccttgt tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca
     9241 gggggtttat cctcccgcgt ctacagttaa accctatgtg gcggtttcgg cattgagcgc
     9301 cggggtgatc acgcgcaata cgacgctgtt tgacccaggc tggtggcaac tgccaggttc
     9361 ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc
     9421 gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg ggatcgatcg
     9481 cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga
     9541 agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg
     9601 gtatcagggt gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat
     9661 ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg ttcctcattt
     9721 gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacagccgc atgaaccgcc
     9781 cgtcggcgat attcattccg gttactggga gctggcgaaa gacggtatgt acggtgttgc
     9841 taaccgccct aacggtacgg cgcataaata ctttgccagc gcaccgtaca aaattgcggc
     9901 gaaatccggt accgctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa
     9961 aattgccgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc
    10021 gcaagtggct gtcgccatga ttctggagaa cggtggtgcg ggtccggcgg ttggtacact
    10081 gatgcgccag atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga
    10141 aaatccagcg gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa
    10201 acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg
    10261 gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt
    10321 aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca
    10381 cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg
    10441 gtagatgctt tcggtgccat ctctaaaggt gctcaacgct ggctggacct cggtattgtt
    10501 cgttttcagc cgtcggaaat tgccaaaata gccgtaccac tgatggttgc gcgctttatc
    10561 aaccgcgacg tttgcccgcc atcgttgaag aacactggca tcgcgctggt gctgatattt
    10621 atgcccacgc tgctggtggc tgcacagcct gacctgggaa catcaatcct cgttgcgctt
    10681 tccggtctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta
    10741 gtgctggtgg cggcgttcat tccgattctg tggttcttcc tgatgcatga ttaccagcgc
    10801 cagcgcgtaa tgatgctcct ggacccggaa tcagacccac tcggcgcggg ctatcacatt
    10861 attcagtcta aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggc
    10921 actcagtcac agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg
    10981 gcggaagagc tgggattagt gggcattctg attctgctcg ctctctacat tctgctgatc
    11041 atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggt
    11101 ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt
    11161 ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg
    11221 ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa
    11281 agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat
    11341 gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc
    11401 ggtatgtaac ggccccatag ttgaaattag cggggcggat ccgcgtttcg aaccactgaa
    11461 cgcgacggca aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc
    11521 gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaaccag gcagtaacct
    11581 gacggcctct ggcgaagctt tcgatccgac acagctgacg gcggcccatc caacgcttcc
    11641 gatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat
    11701 taatgatcgc ggcccttacg gcaacgaccg cgttatttcg ctttctcgcg cggcagctga
    11761 ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga
    11821 tggttcgctt tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct
    11881 gccagcacct cccgatttaa gcggtggcgc gggaacaagt tcagtgtctg gcccgcaggg
    11941 tgacattctt ccggtcagta attcgacgct aaaaagcgaa gatccgaccg gcgcgccggt
    12001 aaccagtagc ggtttcctcg gcgctccaac gaccttagcg cctggtgtac tggaaggcag
    12061 cgaaccgacg cctgcgccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc
    12121 gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca
    12181 agtcggggcc gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa
    12241 gttcggcgtc cacggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc
    12301 attcgccagc aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt
    12361 acagtcattt attactaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg
    12421 taaatgacgt taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat
    12481 agtagggcac ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    12541 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    12601 tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga
    12661 tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc
    12721 agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    12781 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    12841 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    12901 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    12961 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaacagcta
    13021 cgttaacgcc ctgggcctga aaaacaccca cttccagacg gtacatggtc tggatgctga
    13081 tggtcagtac agctccgcgc gcgatatggc gctgatcggc caggcgttga tccgtgacgt
    13141 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    13201 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggaatca aaaccggaca
    13261 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    13321 ctctgcagtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgtt
    13381 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    13441 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    13501 gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag
    13561 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct
    13621 tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa
    13681 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    13741 attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc
    13801 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    13861 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    13921 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    13981 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    14041 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    14101 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccccctcctc
    14161 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    14221 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatca cccaggctat
    14281 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    14341 ctatccggta ttcacccaag ggcaggcagg aaaagcggag cacattttaa tgccgggtga
    14401 tattccggtg atccagagcg atcgcggtgg gcaggttact tatcacgggc cggggcaaca
    14461 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    14521 cttgcttgaa caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    14581 ggctgacgcg ccaggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    14641 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    14701 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggca
    14761 acccgaagcg acgactaata atattgctcc acgtttactg caaaatattt tagcgctact
    14821 aaacaatcct gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac
    14881 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    14941 tctttatcac ttgcagaaat gcgatgctac gccttgctcc gtaactcacc ccattgatta
    15001 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta g
//
LOCUS       DAZFAH010000106        14906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFAH010000106 DAZFAH010000000
VERSION     DAZFAH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.106"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(170..1081)
                     /gene="gsiD"
                     /locus_tag="ACHJWB_003053"
     CDS             complement(170..1081)
                     /gene="gsiD"
                     /locus_tag="ACHJWB_003053"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4314642.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1084..2004)
                     /gene="gsiC"
                     /locus_tag="ACHJWB_003054"
     CDS             complement(1084..2004)
                     /gene="gsiC"
                     /locus_tag="ACHJWB_003054"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4314643.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2022..3560)
                     /gene="gsiB"
                     /locus_tag="ACHJWB_003055"
     CDS             complement(2022..3560)
                     /gene="gsiB"
                     /locus_tag="ACHJWB_003055"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4314644.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     HELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3580..5451)
                     /gene="gsiA"
                     /locus_tag="ACHJWB_003056"
     CDS             complement(3580..5451)
                     /gene="gsiA"
                     /locus_tag="ACHJWB_003056"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4314645.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5438..6403)
                     /gene="iaaA"
                     /locus_tag="ACHJWB_003057"
     CDS             complement(5438..6403)
                     /gene="iaaA"
                     /locus_tag="ACHJWB_003057"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4314646.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALLFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6607..7842
                     /gene="moeA"
                     /locus_tag="ACHJWB_003058"
     CDS             6607..7842
                     /gene="moeA"
                     /locus_tag="ACHJWB_003058"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4314647.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISTGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7842..8591
                     /gene="moeB"
                     /locus_tag="ACHJWB_003059"
     CDS             7842..8591
                     /gene="moeB"
                     /locus_tag="ACHJWB_003059"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4314648.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8667..9329)
                     /gene="fsa"
                     /locus_tag="ACHJWB_003060"
     CDS             complement(8667..9329)
                     /gene="fsa"
                     /locus_tag="ACHJWB_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4314649.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9460..10359
                     /gene="ybiY"
                     /locus_tag="ACHJWB_003061"
     CDS             9460..10359
                     /gene="ybiY"
                     /locus_tag="ACHJWB_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4314650.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10365..12380
                     /locus_tag="ACHJWB_003062"
     CDS             10365..12380
                     /locus_tag="ACHJWB_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFO4314651.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEHML"
     gene            12526..13341
                     /gene="ybiV"
                     /locus_tag="ACHJWB_003063"
     CDS             12526..13341
                     /gene="ybiV"
                     /locus_tag="ACHJWB_003063"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4314652.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13493..14758
                     /gene="ybiU"
                     /locus_tag="ACHJWB_003064"
     CDS             13493..14758
                     /gene="ybiU"
                     /locus_tag="ACHJWB_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4314653.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLEDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3615 a         4115 c         3797 g         3379 t
ORIGIN      
        1 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgcggct
       61 ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact tgcttagggc
      121 aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac tatcctttaa
      181 ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca
      241 gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata tccgctcgtg
      301 cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg ccgagaaatg
      361 acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag
      421 acgagacggt cccaggcaag atatgacgca ataaaatggt catatcgctg gcaccaatac
      481 tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccaggc
      541 gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc
      601 ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc
      661 aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac aagcccagca
      721 acgtccctat cgccgcaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg
      781 caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca aaccagtgct
      841 gcaaagaagg tccattattc agattgtcat aatcaaaata attttcggcg tcatagggag
      901 cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat gcggcggtca
      961 tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgcacctggt
     1021 caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca
     1081 tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt
     1141 gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg
     1201 catttctacg gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac
     1261 cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat
     1321 catcgcgtta cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct
     1381 cgcggtacgc atataatctt cgcttaaaac atcgacaaac gacgcgcggg taaagcgcgc
     1441 catcacggct gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact
     1501 gtctgctccc acggtaggca gccagcccag ttcaacggag aatacctgaa ttaaaagcat
     1561 ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc
     1621 cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc
     1681 ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg caatctcatc
     1741 ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat
     1801 atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc
     1861 tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat
     1921 atgcacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca accccagtaa
     1981 gcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa atccgcgtct
     2041 tcaaagctga agccggtgtc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc
     2101 gacaccagtt tttctaccac cagcgggatc cacggcgact cttgccagat gatatcctgc
     2161 gccgccttat acaagcgggt cttttccgcc ggatcattag ttttcagtgc ctgagccagg
     2221 aagtcgtcca cctgtttatt gctgtaaaat gcggtattaa acagcgtcgg tggccagttt
     2281 tgcgaggcga acagtggcga tagcgcccag tccgcttcgc cggttgaagc cgaccagcca
     2341 gtgtagaaca tccgcacgcc gctctctttt tgccctttac cttcaacttc tgccgcccgc
     2401 tgtccggcat ccatcgcagt cacctggact ttaatcccga cctgcgctaa ctgctgctgg
     2461 gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtactg
     2521 aaaccgttgg gatatcccgc ctctttcagt aattcgtgcg ctttcactgg atcgtaaggc
     2581 cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt agctggcgtt
     2641 gcatagcctg caaaggcaac tttcaccagc gccggacggt taatggcgta attcagcgcc
     2701 tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat ataacgctgc
     2761 ataattgacg gactggccat caactcgata tttttgtttt tctccagcag tgtggcctgc
     2821 tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg
     2881 ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc tggctgccag
     2941 taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac
     3001 ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgccggt
     3061 gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg
     3121 agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt cttatacagg
     3181 ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc tttcaccgcc
     3241 gcggcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtaa
     3301 gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct
     3361 ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca
     3421 tttgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac cacatctttg
     3481 gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac taacccacta
     3541 cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta
     3601 ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc
     3661 cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg
     3721 cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg
     3781 atgctgcggg ttttcgaaga ccgcgcgccg tggaccaatt tcaacaattt gcccgagata
     3841 catcaccgcc acacgatgac taatccgctc taccacggcc atatcgtggg agataaacag
     3901 atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat
     3961 agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat tcaatgccaa
     4021 cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca
     4081 ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcgtc
     4141 tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg
     4201 acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc
     4261 ctgaagtttg ccgggtgaca aggtatcgat tctctgaccg ttaaagataa tttcgccgcc
     4321 ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc
     4381 gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg
     4441 cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgacaagatt
     4501 acgcactcgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggggcctg
     4561 tttcgctgga tgttcaagcg atatcaacgg gaaacgtcgg ggataatcta accctttcat
     4621 cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg
     4681 aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg
     4741 atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat
     4801 ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc
     4861 ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat
     4921 cactcgctgg cgcatcccgc cagagagttg atgcggataa cgtgaaagaa tggtttgtgc
     4981 ctcaggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact
     5041 ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa ataccgggtt
     5101 cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgaa catggcgcat
     5161 ttgtgcagcg ctctgctcgc taagttcaat cacttcgcga ctgcgccgct gcaacagcat
     5221 tttatcgcac tgtactaaac cgcccgcctg ttccaacagg cgcatcaatg ccaacgcagt
     5281 cactgactta ccggagccgg attcgccaac aattgccagc gtctcaccgc gttgcagact
     5341 aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattcag
     5401 attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca acggtgtccc
     5461 ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca
     5521 ttccttcggt gttaaacagt agcgcgacat tcccttcatg gtcgatagcg attaagccac
     5581 cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac
     5641 ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga
     5701 cttcgcccgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggcac
     5761 ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg cctgtggacg
     5821 tggctgccgc caaattgccg tctaaatcca acgccacggc ccccacggtg cccattttct
     5881 gtttttcatc cagtggcgca ccgctatggt cgaggacggt tgccccttcc ttgcgcgctg
     5941 ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggcgagacg cgctccatgc
     6001 cacgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct
     6061 ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc gccaccgcac
     6121 cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt tcatcacgcg
     6181 taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga cgcaccgctt
     6241 ccgtcaccac atccagcgca ctttcgcccg cttccagcat tttctgcccg gtttcaacaa
     6301 tggcagacaa cgcctcaatg tagcgtaatt cctgttgcag actcatctgc gcgcggctaa
     6361 ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc atccattaag
     6421 ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt
     6481 gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc
     6541 atacagtctg tggcgttttt ctgacataat aggcaaattc gattttgcct ccgcaggagt
     6601 gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt
     6661 tctcgcgtca ccccactgac cgcccaggaa acgctgccac tggtacagtg ttttggtcgt
     6721 attctggcga gcgatgtcgt ttcgccgctt gatgttccgg ggtttgataa ctccgcaatg
     6781 gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc cgttgccggt
     6841 aaatcctttg ccggtcagcc gtatcatggt gaatggcctg cgggcacctg cattcgtatt
     6901 atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa
     6961 caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt
     7021 cgcggtgaag atatctctac aggtgcggtt gttttcccgg cgggaactcg cctgactacc
     7081 gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg
     7141 cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac
     7201 ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca gctgggttgc
     7261 gaggtaatca acttagggat tatccgcgac gatccccatg ccctgcgcgc cgcatttatt
     7321 gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt gggtgaagcg
     7381 gattacacca aaacgattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt
     7441 aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg cggcctgccg
     7501 ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaaa
     7561 ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc
     7621 ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg caacgccgac
     7681 ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag ctcctttagt
     7741 ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggaagt gggcgaatgg
     7801 gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatgacggaa ctcagcgatc
     7861 aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg
     7921 aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt
     7981 cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gactcggttt
     8041 cgctctcgaa tctgcaacgc cagacgctgc acagtgatgc tacggtcggg caaccgaagg
     8101 tagaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt acgccagtca
     8161 atgcactgct tgatgacgct gaacttgctg cgatgattgc aaaacacgat ctggtactcg
     8221 actgtacgga taacgttgcg gtgcgtaatc aactgaacgc tggctgtttt gccgcgaagg
     8281 tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc ttcacttatc
     8341 aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcattaacct
     8401 gcgtagaagc aggcgtgatg gcaccgttga tcggcgtaat tggttcgttg caggcaatgg
     8461 aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc gtcatgtacg
     8521 atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt
     8581 gcgggcagta attgccacct ccaggccaga taagacgcgg cactccgcca cgtcatcagc
     8641 aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc tcccagccag
     8701 tcctgttcaa acttcgccac agcggcttcc accgccggat agctaatcat ctgttgtgcc
     8761 acatccagtg gcagcgtaat tgattcacat cctgccagta agcagtccag cgcctgacgc
     8821 ggggttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt caataactgg
     8881 tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg attaacgtaa
     8941 ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgctgc gccatatacc
     9001 gcggttccca gcgttggaat cccttccgct tttaacatct taatagctgc cagcccctcg
     9061 gcggtcaccg gaactttcac cacgatatcc gcaataatag aacgcaattt acgtgcgtca
     9121 ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc ctgaccgccc
     9181 atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc ggcgataatg
     9241 cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt caccgcaaca
     9301 acgtctgaag tatccagata cagttccata accatcctca aaatgtactt tttgtctgaa
     9361 tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta gctttcattc
     9421 gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa tattcagcgc
     9481 tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg ctgttcgctg
     9541 ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca cgcaggatct gctgtatgac
     9601 gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg ccgcgccgga agtgattgag
     9661 cgcgcgctga atggtttgct tattcatcgg gaaaagttaa ccccggagca tctgacggcg
     9721 ttaaccgact gctgtccgac acaggcatta accgtgtgtg gtgaagtgaa aagcgttgag
     9781 gagatcatga cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg cggcggttta
     9841 acgctttcgg gtggtgagcc ctttatgcag ccggaaatgg cgatggcgct actgcaagcc
     9901 agccacgagg caggcattca tactgcggta gaaacctgtc tgcatgtgcc gtggaaatat
     9961 atcgcccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca cgttgccgac
    10021 gcgccgttta aacagtggac cgacggtaac gccgccagag tgctggataa cctgaaaaaa
    10081 ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg ctttaatgcc
    10141 gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca cgttggcgaa
    10201 attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact taatctgccc
    10261 tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc tcgactttgc ccagcagtat
    10321 gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc acactgaaac
    10381 tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat attgtgaaac
    10441 cgccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa catctcgata
    10501 agccgatccc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat cgcaccatct
    10561 ggatcaaaca cgatgagttg atcattggca accaggcaag cgaagttcgc gccgcgccga
    10621 tcttcccgga atatactgtc tcgtggatcg aaaaagagat tgatgatctg gcagatcgtc
    10681 ccggtgctgg ctttgcggtg agcgaagaga acaaacgcgt tctgcatgaa gtgtgcccgt
    10741 ggtggcgcgg tcagaccgta caggatcgct gctacggcat gtttaccgat gagcaaaaag
    10801 gtctgctggc gaccggaatc attaaagcgg aaggcaatat gacctccggc gatgcacacc
    10861 tggcggtgaa tttcccgcta ctgctggaaa aaggtcttga tggtctgcgt gagaaagtag
    10921 cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga tttacatggc gaacaattcc
    10981 tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt ttcgctgccc
    11041 tggcgcgtga aatggctgct accgaaactc gcgaaagccg tcgcgatgaa ctgctgacga
    11101 tggcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg caggcgctgc
    11161 aactgtgtta cttcatccaa ttgattttgc agatcgaatc taacggtcac tcggtatcgt
    11221 ttggtcgtat ggaccagtat ctctacccgt actatcgccg cgacgttgaa ctcaaccaga
    11281 cgctggatcg cgaacacgcc atcgagatgc tgcatagctg ctggctgaaa ctgctggaag
    11341 tgaacaagat ccgctccggc tcacactcaa aagcctctgc gggaagtccg ctgtatcaga
    11401 acgttaccat tggcggacaa aatctggttg atggtcaacc aatggatgca gtgaatcccc
    11461 tctcttacgc gatcctcgaa tcctgcggtc gcctgcgttc cactcagcct aacctcagcg
    11521 tgcgttacca tgcaggaatg agtaatgatt tcctcgacgc ctgcgtacag gtgatccgtt
    11581 gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt gatcccggaa tttattaaac
    11641 tcggtattga accgcaggac gcttacgact acgcagcgat tggttgtatc gaaaccgccg
    11701 tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc gcccgcgtga
    11761 tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg caaagtgttc ctgccacaag
    11821 aaaaagcgtt gtcggcaggc aactttaaca atttcgatga agtaatggac gcgtgggata
    11881 cgcaaatccg ttattacacg cgtaaatcaa tcgaaatcga atatgtcgtc gacaccatgc
    11941 tggaagagaa cgtacacgat attctctgct cggcgctggt ggatgactgt attgagcgag
    12001 cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg caggttggca
    12061 ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtatttgaa cagggcgcga
    12121 ttggtcagca acagctggct gccgcactgg cggatgactt cgatggcctg actcatgagc
    12181 aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat gataccgtcg
    12241 atacgctgct ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg
    12301 cgggctattc cgccttcttc accgcgctct ctccagacgc acaggacgat atcatcgccc
    12361 gtactgaaca tatgctgtaa tctcctcggc ccggcggcgt cgtcgccggg ctaaatcatt
    12421 cacatcatca atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt
    12481 gttattacac tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt
    12541 atcgtcacag acatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacat
    12601 tttatggcgc aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt
    12661 aatcagtatt accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc
    12721 gcggaaaacg gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc
    12781 cgacatgaat cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc
    12841 gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg
    12901 atggcaaaac actaccatcg cctgaaagct gtaaaagatt atcaggagat tgacgacgta
    12961 ctgttcaagt tttcgctcaa cctgccagat gaacaaatcc cgttagtgat cgacaaactg
    13021 cacatagcgc tcgatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg
    13081 attattcccg gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg
    13141 tcaccgcaaa atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg
    13201 gcgcgttatt cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac
    13261 gctaccgatg ataataatca tgaaggcgcg ctgaatgtga ttcaggcggt gctggataac
    13321 acatcccctt ttaacagctg acctctctac cggagccgtg cgctccggtt ttcccttctt
    13381 tttacatcct gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttata
    13441 ttatcacatg caaataagaa tggtttcgaa tgaaagttaa tgagaggtgg ttatggcttc
    13501 tacttttacc agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca
    13561 cgcgctgcgg gcgcagcttg gcgacgtcca gcagatcttt aatcagctaa gcgatgacat
    13621 tgccacgcga gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt
    13681 gctgtcttat gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa
    13741 acgtcgcggt tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga
    13801 tcagtcgatg ctggactatc tggaccgcaa ccgctttgac gaggtctaca aaggccccgg
    13861 cgataatttc ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc
    13921 gcaggcgcaa atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa
    13981 tcgtctgtgg acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat
    14041 ctaccctgac cgtatccgcc gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca
    14101 taccgactcc ggggcgctgg aacgctggct gcttccagcg tatcagcgcg ttttcgccaa
    14161 cgtctttaat ggcaatctgg cgcaatatga tccctggcat gcggcacatc gtacggaagt
    14221 tgaagagtac acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg
    14281 gacagcgctc tctgatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga
    14341 agccatggcg tacgtactgt tacgtccgct gcttgaagat gtgccggagg atgaactgtg
    14401 cggcgtagcg cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga
    14461 ggcgctaacc agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt
    14521 catccattcc gttgcccccg ttgaaaatca acagggttgg ggcaatgtga tgtacattcc
    14581 tgcggcaccg atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga
    14641 aaaaggcgca tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg
    14701 ctttacgctt gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga
    14761 gttaatggaa gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct
    14821 gacaagtgcg ctttcttaat gcctgatgcg gcgttaacgc cttatctggc ctacaaaagt
    14881 atgcaaattc aatatattgc agagtt
//
LOCUS       DAZFAH010000107        14600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFAH010000107 DAZFAH010000000
VERSION     DAZFAH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.107"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(140..1564)
                     /gene="patD"
                     /locus_tag="ACHJWB_003065"
     CDS             complement(140..1564)
                     /gene="patD"
                     /locus_tag="ACHJWB_003065"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4314654.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1586..2380)
                     /gene="ydcV"
                     /locus_tag="ACHJWB_003066"
     CDS             complement(1586..2380)
                     /gene="ydcV"
                     /locus_tag="ACHJWB_003066"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4314655.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2370..3311)
                     /gene="ydcU"
                     /locus_tag="ACHJWB_003067"
     CDS             complement(2370..3311)
                     /gene="ydcU"
                     /locus_tag="ACHJWB_003067"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4314656.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3312..4325)
                     /gene="ydcT"
                     /locus_tag="ACHJWB_003068"
     CDS             complement(3312..4325)
                     /gene="ydcT"
                     /locus_tag="ACHJWB_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4314657.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4343..5488)
                     /gene="ydcS"
                     /locus_tag="ACHJWB_003069"
     CDS             complement(4343..5488)
                     /gene="ydcS"
                     /locus_tag="ACHJWB_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4314658.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5733..7138)
                     /gene="ydcR"
                     /locus_tag="ACHJWB_003070"
                     /pseudo
     CDS             complement(5733..7138)
                     /gene="ydcR"
                     /locus_tag="ACHJWB_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7217..7633)
                     /gene="hicB"
                     /locus_tag="ACHJWB_003071"
     CDS             complement(7217..7633)
                     /gene="hicB"
                     /locus_tag="ACHJWB_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4314659.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7679..7855)
                     /gene="hicA"
                     /locus_tag="ACHJWB_003072"
     CDS             complement(7679..7855)
                     /gene="hicA"
                     /locus_tag="ACHJWB_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO4314660.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8077..8307
                     /gene="yncJ"
                     /locus_tag="ACHJWB_003073"
     CDS             8077..8307
                     /gene="yncJ"
                     /locus_tag="ACHJWB_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4314661.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8399..10360)
                     /gene="rlhA"
                     /locus_tag="ACHJWB_003074"
     CDS             complement(8399..10360)
                     /gene="rlhA"
                     /locus_tag="ACHJWB_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4314662.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10433..10969)
                     /gene="sutR"
                     /locus_tag="ACHJWB_003075"
     CDS             complement(10433..10969)
                     /gene="sutR"
                     /locus_tag="ACHJWB_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4314663.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGKLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11022..12236
                     /gene="ydcO"
                     /locus_tag="ACHJWB_003076"
     CDS             11022..12236
                     /gene="ydcO"
                     /locus_tag="ACHJWB_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4314664.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12276..13484)
                     /gene="insQ"
                     /locus_tag="ACHJWB_003077"
     CDS             complement(12276..13484)
                     /gene="insQ"
                     /locus_tag="ACHJWB_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4314665.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSLQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            13492..13905
                     /gene="tnpA"
                     /locus_tag="ACHJWB_003078"
                     /pseudo
     CDS             13492..13905
                     /gene="tnpA"
                     /locus_tag="ACHJWB_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(14016..>14600)
                     /locus_tag="ACHJWB_003079"
     CDS             complement(14016..>14600)
                     /locus_tag="ACHJWB_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4314666.1"
                     /translation="DKYSGFLNNYSDLKETTSATGKPVLRWVDPSFDQSTYDSIVWNP
                     ITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPVVTTAGPRSLIFRGAITGVD
                     TSKEGLQFYEVVPVALVVAGTQMAIGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEG
                     KDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT         3564 a         3867 c         3556 g         3613 t
ORIGIN      
        1 acaggatgaa aaatcgccat caaacattta gcctaatttt tacgctagtt attaatgtaa
       61 tcattctaat acttaaccgc cattaaactc agaacctaaa acgatagtgg ccgtcctata
      121 acagccacta acatctaact taatgtttaa ccatgacgtg gcggacgacg gtgtaatcct
      181 ccagcccata aagtgacata tccttgccgt aaccagaaag tttctgccca ccgtgcggca
      241 tttcacttac cagcatgaaa tgggtattga cccaggtaca accatattgc agccgtgcgc
      301 tgacgcgatg cgccctgccc acatctttcg tccataccga agatgcaagt ccgtactggc
      361 tgtcattcgc ccagttcacc acctgttctt cgttgtcgaa gggcgtaaca ctcactactg
      421 gaccaaatac ctctttttgc acgatggcat cgtcctgtaa tgcgccagcc agcagcgtcg
      481 gcgcatagta atagccatta cccttgcgct tttcaccgcc agtgatcact ttgatgtgcc
      541 ctgtcgcttt cgcctcttct actgccttgc tgacgcgttc gagatgcgcc agcgagctta
      601 aaggtccaag ctccgtagac tcgtcatccg gcgcaccaga ttttaacgtt gccaccgcag
      661 cacccagttt ttccaccagc gtatcgtaaa tgcctttttg cgcgtagatc cgacaagccg
      721 cagtacaatc ctgtccagca ttgtaatagc caaatgtacg tacaccttcg accactgctt
      781 caatatccgc atcatcaaaa acaatcactg gcgctttgcc accaagttcc atatgagtac
      841 gcttaatgga cgacgcggta tggctgatga tgtgctcgcc ggtggcgata gagcccgtca
      901 gcgacaccat ccgcactttg ggatgaccgg tcagcggatc acccaccgtt ttgcctctgc
      961 caaacagtat gttaatcacg cctgccggga agatatcttt cgccagctct gccaacttca
     1021 acgcggtcag cggggtaatt tctgatggtt taagcactac gcagttccct gccgccagcg
     1081 ccggagcaag tttccacgcg gccatcatca gcggataatt ccacggtgcg atagaagcca
     1141 cgacccccaa cggatcgcga cggatcatcg aagtatgacc ttcaagatat tcacctgccg
     1201 ccagaccatt cagacagcgc gccgcacccg cgaaaaagcg aaaaacatcg acaatcgccg
     1261 ggatttcatc attgaacgca ctatgcagcg gtttgccaca attacgggac tccagttcgg
     1321 caaaaacctg accattttct tcgataacat cagccagttt cagcagacat tccgcacgca
     1381 ctttcggcgt ggtttgcccc cattcggcaa atgctgcatc tgccgcgcgc acagcagcat
     1441 cgacctgctc tgcggatgcc tcggcaattt ccagtaaaac gtcccccgtt gccggattat
     1501 agacaggctg tttttcccct tcgccgctaa ccagttctcc gttaatcagt aacttatgtt
     1561 gcatagcata ttcctgtatc agtggttatt gaccattgtc gccttcgcgg gttagccacc
     1621 aggcccccag gatcggcaag gttgttacca acataaccag cagtgccacc acgttagtta
     1681 ccggtacatc acgcggtcgc ccaagctgat tgagcaacca caacggtaac gttcgttcat
     1741 gacctgccgt aaaggtcgta acgatgattt catcgaacga caaggcaaac gccagcattc
     1801 ctcctgccag taacgccgaa ctgagattcg gcaacactac gtagcggaag gtttgccagc
     1861 cattggcccc aagatccata gacgcctcaa ccagactcca ggaggtgcgg cgaaaacggg
     1921 cgatgacatt gttaaacacc acaactacac aaaaagtcgc atgaccgacc acgatggtga
     1981 aaaatcccgg ctccagattg atggttttaa aggcggttaa taacgccaga ccagtgacaa
     2041 tgcccggcag cgcaatgggc agcagcagta acagcgaaat ggcgtttttg ccgaaaaagt
     2101 ctcgtcgcca cagcgcggca gctgccagcg tccctaacac cagcgcaatt aatgtcgcca
     2161 gcgccgccac tttaagtgac agtgtcacgg catcaagaat atcactacgc tgtgctgcca
     2221 cgctaaacca gcgcagcgtc aggccctgcg gtggaaaact aaacgccgca tcttcagtgt
     2281 taaacgcata agcggcgata atcaggatgg gaaaatgtag gaaaaccacg ccgccccagg
     2341 ccgccagttt gaggaaaaac ggtgcgcgtt cagagtgcat cgaacgctcc cagacgtttc
     2401 acgaacgcca ggtacagtgc gatgagaaca atcggcacca gggtgaatgc cgccgccatc
     2461 ggcatattgc caatcgcccc ctgctgggaa taaaccatat tgccgataaa atatcctgga
     2521 ggaccaacca gctgcgggac gataaaatcg cccagtgtga gtgagaaggt aaagatagag
     2581 ccagcggcaa tacccgggat tgccagcggc agcaccacat agcgaaaggt ttgtcgtgga
     2641 cgtgcgccga gatcagccga cgcctgcaac aatgacggcg gcaaacgctc aagcgccgcc
     2701 tgaacgggca ggatcatgaa cggcaaccag atatagagaa acaccagaaa gcgccccagc
     2761 ccggaagttg acagcgtatt tccgcccacc gcaggtaatg taaggaacgc agtcagcagt
     2821 ggttccagcc caagatgttg taaaaaccac tgagccacgc catcttttgc cagcaataac
     2881 gtccaggcat aggctttaac aatgtagctc gcccacatcg gcaacattac cgcaatataa
     2941 aaaaaagctt tcattttccc gctggtatag cgcgccatat accacgccat tggaaaagcc
     3001 agaatggcgc tggcgatagt gactgccaca gccatagtca gcgtgcggag aatgatgtcg
     3061 taattcgccg gattaaacag cgcacggata ttcgccagcg tcagttccgg cgttaccgac
     3121 atggtgaaat cgtcaaaagt ataaaatccc tgccataaca gtgtcagcag cgagccgaaa
     3181 tagacaatgc caaaccacat tagcgggcca agcagcagta aaaacagccc cagccccgga
     3241 ttacgccaga agaaaccgga caccttaccc agacctggac gtgaaggtga ttgcaataca
     3301 ttcatcgcca ttcacctctc ctcaaccagc ggcaccatca catcacgcga ccaggaaacc
     3361 atcacctgtt gtccgggcgt gagcgtggca ggcagttctt cgcctgtcat attggcctga
     3421 ctcacaagca gtttttcacc gccgttcaat ttcagttcaa aacgagttgc cgcgccctga
     3481 tattgcaccg cctggatcgt gccattggcc tgcagttcac caggggtgtt gaggcggata
     3541 tgttccggtc gcagggcgaa gcttcccgtc atgccacaaa gtttctctgc catcagtcca
     3601 tcaaaaacgt tggatgtccc gacgaatccg gcaacaaacg gcgtgcgcgg gcgcatatag
     3661 agatcgcgcg gggaatcgac ctgctcaatg cgtccattat tgaaaaccgc cacacgatcg
     3721 gacatcgata acgcttcgcc ctgatcgtgg gtaacgaaga taaaagtgat accgagagac
     3781 tgttgcagtt ttttcagttc cagctgcatc tgctcacgca atttgagatc cagtgcgccg
     3841 agcggttcat ccaacagcaa tacacgcggt tcattcacca atgctctggc gatagcaacc
     3901 cgctggcgct gaccaccaga aagttgtgac ggtttacgtt gatgtacaaa ccccaacgcc
     3961 actttctcca gcgcctcttg cgccattgcg tgccgctgct ttttattcac gcctttgacc
     4021 atcagcccat aggcgacatt gtcaagaatc gacatatgcg gaaatagcgc gtagtcctga
     4081 aagacagtat tcacgtcccg ctcccacggt ggcagattgc tggcgggttt accaaagata
     4141 gagatagccc cgccggaaag ctgttcgaag ccagcaatca ggcgcaggca ggtggttttg
     4201 ccggagccgg acggccccag catagagaag aactcaccat cttttatcgc aatactgacg
     4261 ccatctactg cgcgcacgtc accgtacaac cgcgagacgt tgtcaaactc cactgcgtac
     4321 gtcataaagc accccagcga agttagcgac cgcccataat ggcaatgtaa tcctgcgtcc
     4381 agcgactgta gggaacaaac ttgccccctt ctgctatagg cgttttccag aaggcgattt
     4441 tgtcgaaata gttaaaaccg ttggtttcac aacctttttc gcctaataac ggactggctt
     4501 tacacccttc cggcactacc ggtaacgagc caaaccaggc cgccacatcg ccctgcactt
     4561 ttggcgttaa tgaccagttc atccatttgt aggcgcaaac cggatgtttc gcttcgctat
     4621 gcagcatggt ggtatcagcc caaccggtaa caccctcctt cgggaaaacg gtagcaacag
     4681 gctggccttc ggctttcagg gcgttggcct gatagggcca ggcactggaa gcaaccacac
     4741 cctcgttttt gaaatcgctc atttgcacgg tagtgtcatg ccagtagcga tggatcaaac
     4801 tgtgttgagc gcgcagcact ttcagcaccg cctggtactg ttcttcggtg agttgatacg
     4861 gatcgctgat gcccaactgc ggctgagtgg ctttaacgaa caacgcagcg tccgcaatgt
     4921 agatagggcc atcataagcc tgaacgcggc ctttattgct cttgccgtcc ggcagatttt
     4981 gctcaacaaa aaccacttgc cagctatccg gcggcgtcgg gaaggtttta gtgttgtaca
     5041 tcagcaggtt cggcccccat tggtaaggtg tgccgtaaac tttgccgcca acattaaacc
     5101 agtcgccttt aaccacgcgc ggatcgagcg ttttccagtt gggaatcaag gcggtattaa
     5161 tcggctgcac gcgtttgccc ataatcaaac gcagcgaggc atcgccggat gccgtaacca
     5221 gatcgtaacc ccctttggtc atcagactga ccatttcatc ggaagtcgcg gcggttttca
     5281 cattcaccgc gcagcctgtc tctttttcaa attgcgttac ccagtcgtat tgtttatcag
     5341 tttgtccgcg ttcgatgtat cccggccagg cgataatatc cagtcgccct tccggtttat
     5401 ctaaattggt aggtggttcg gcggcgtgag cggtcattat tgtcatggtg agcgcacaca
     5461 ggctgctgcg ggcaaatgtc ttgctcataa ggtcttactc ctgtcgtaat taattgttca
     5521 gccgccgtgc cgtaaaaata tctccttgtt taaccataga cggcggcttg caggcacacc
     5581 ttcgcccctg agaaaattta atcaggttgt gagctaccgc gcattatgcc gtctgtaagg
     5641 ttatcttctg gagtatagac aaggggttag gaaatatgac tgctattcga aattcatatt
     5701 acccaatcta agaggaatta aattaaacgc tattacagcc gttcttgaat aagtttgcct
     5761 aattgtttta ccgcctgttc ttcacgttct ccccactgcc acgcggtatt aaaacgaaaa
     5821 aaacgtgacc agttttcacc ggtagaaaac attttacccg gcgcaatact gatatgatgc
     5881 gtcagtgccg ccaggcttaa ttcgccggca tctaacggct cggggagctc cagccagaga
     5941 aaataaccgc tgtcactatg atgaatttta acttccgcag gcagataacg cagcagtgcc
     6001 tgccaggcac gttgtttacg ttccgcaagc tggcgacgca ggcgacgaag atgggcgtcg
     6061 tatcggcgcg tggaaaggta atccaccagc gcaagttgca tcggtgagct ggtggaaagc
     6121 gtactcatca actgcaagcg ttgaatttta cgtgcatgtt ttccggcggc gacccaacca
     6181 atacgaaaac caggcaccag acatttcgaa aacgaagagc aatgcaaaac gccatcgtgg
     6241 cgatcccacg ctttcgcagg cagcggtttt tcccgtccaa aataaagttc gctgtaaacg
     6301 tcatcttcaa tcagcgttac gttgtactga ttgagtaacg ccaccagttg tgcttttttt
     6361 gcggcgttaa ggtaaatccg agtgggttct ggctattagt catcagccag cacgctttca
     6421 ccggataatc ctgcaacgcc agttccagcg cctgaagatc gatcccttct ttaacatccg
     6481 tcgccaccga taacgccttc agccgtagcc gctccagcgc ctgcaacgca ccgtagaaac
     6541 aaggattctc tactatcacc caatcgcccg gttcagttac cgcttgcaaa ctgaggttta
     6601 atgcctctaa cgccccggca gtaatgacaa tttcatcagg agaaatggtg atgccctgta
     6661 aggcatagcg acgagcaata gcctgacgca gttctgcgtt tcctggcggt aagttttcaa
     6721 tcacgctcat cgctgtggcg gttttgctta cctgcgccag cgagcggttt agttgttgga
     6781 gggggaaaag tcgcgggtcg ggaaaggccg aggcaaacgg aacgaccgac ggatcgcgac
     6841 tggcctgcaa catatcaaaa atataagtgt tgatatcgac tgcttcatct cgagtgactg
     6901 gaatgactgg cgctttcggc atttttattg cctgtggcgc aacgtaataa cccgactgcg
     6961 gtcgtgcgat aatatatccc tgactttcga gcaactgata ggcatggctg acagtcataa
     7021 agctcatgcc tgaaagcgcc acctggtcac gcaacgaagg caaacgatcg ccgggttgcc
     7081 agatacccga cgcaatctgc tcgcgtaatt gttctgcaag ctgctggtat tttttcatta
     7141 tcgaatcgta attatgtgca gatgattcgg cagtctatat cagttgttaa aaatgacaac
     7201 ttttcgttaa ctgtaattaa accatcacca gcgataactc tttgccaagc gcctttgccg
     7261 cgagctggac ggcgtcgatt tttgtcgcat gatgcaagtt aaataggcga gtaatctcct
     7321 gtttaggttt gccaattcgc ctggctaact cttgctgagt gatttctgac tgtaaaaaag
     7381 catttaacag caatacctta gaggcgacgc tcaaaggtac ttcaataaag tgatcatgac
     7441 tatttaatgg cgaaggtaaa gggataagct cgttatcttc aaaataaaaa tcaaatgcgg
     7501 tcagtaaagc atcttttgcc gcttccatcg cttcagcgac agtttcgccc tgcgtcaacg
     7561 cttcagggat atccacaaaa gaaaccatat aaccgccttc cggcgcgggt gtaagagtga
     7621 cgggataacg cataatttcg actgtccctc gcatgtcatt aaccttttta attggcgatt
     7681 aactcaaacc gagttgtttc aggattgctt tacgcaatgg ttctttaatc tcatcgcagg
     7741 ggtgacgcgg catgacactg cgcctcccat gaaacctgag tttcaaatgg ttgctgccat
     7801 tcgctacatc gacgccctga gattcgagcc aacgtctgaa ctcgctttgt ttcacatcgc
     7861 ctccctgccg ctgagtatat caacaaagta aacattagtg tttaccttcg tgtgtaattt
     7921 gctcgaaaat agcactaaat cgccgcaacg gcagaatgcc atgcaaaatc aggaaggaag
     7981 attccagaat ttctttgcgc taatttgcaa aactgaataa taacctcaag aaacgatcgg
     8041 catcatagcg gcaaaaggta agaaaaaggt ggcaggatgt ttacgaaggc gttatcggtt
     8101 gtcttattaa cgtgtgcgct gttctcagga caactcatgg cagggcacaa aggacatgaa
     8161 tttgtgtggg taaagaatgt ggatcatcag ctgcgtcatg aagcggacag cgatgaattg
     8221 cgtgctgtgg cggaagagtc ggcggaaggt ttgcgcgagc atttttactg gcaaaaatcg
     8281 cgcaaaccag aagcgggaca acgttgagtc atttgttgcc ggatgcggcg tgacgcctta
     8341 cccggcaggc aatatcatgc agattcaatg agttgtgagt aaccagaaaa ctggcgtttt
     8401 acccttttcg cttaggcaat gttttcagca gctcatccgg acttacggat gccactacgc
     8461 ttcccggtaa cggcattttc agtatgtgat ttttgatttt gccaatgacg tgcatctcgc
     8521 atgggcggca atcaaacttt agcgttaata cttcatcgcc gttaaccagt tgcattggcg
     8581 tcgccttcca gcttttgata ttgcctttcg cctgtttcgg gcacagatta aaggcaaagc
     8641 gcagacaatg cttggtgatc atcaccggga cttcgccctt ctcttcatgt gcttcatacg
     8701 ccgcgtcaat cagctgcaca ccatagcgat gataaaattc acgcgctttc tggttgtata
     8761 cgttcgcgag gaaactcaga tgcgtttgcg gataaaccgg cgcaggatca gcaaccggtt
     8821 tacggctgcc gcgctggtaa ctggcaagac gcgcagcatc cagcatgtca gcagcttcac
     8881 ggcggaactg gtttaacaga ctgtttggta caaacagcgc ccccggcaaa ttaatttgca
     8941 catcgcgggc gtaatagagg gtttgcccca gttttgccag accatccttc agattgttca
     9001 ttgctttttc ggcgttattg gcttcgtcga actgcccgtc cagcgtatgc gtgatgctga
     9061 caccctcttc actggtgagg gtcagaatca gttgttcctg ccagccgccc agttcaatgt
     9121 ctaccgccac ccgacgttcg ctggaggttt ttgtcagtgc ctgctgccag ttatgatcaa
     9181 gattacggtt tagtgggtga tgtggacgaa ttttgtgcaa atctgctggc atttcattgg
     9241 gccagacgcg gtactgattt tctccggttt tctcgaccgt attggcacga aaaccgacga
     9301 cttcacgttt aatcaacacg ttcaggccat cgccatttgc cagtggctcg gtaacggcaa
     9361 catcgagatg atctttcgcc actttcaata cttcgcctac cggcaggccg ataaatttcg
     9421 gcgaatcgaa cgcgccaata tcgcctttac gggcattcac aaaataatct gtgctaccac
     9481 ggtggaaagt cttttccgtc gatggaacaa agaaatgttc agtacgacct gatgaagcgc
     9541 gcgccagatc gccacgttct tcaataatgg catcaagcat ctggcgataa tgggcggtga
     9601 tattcttcac gtagctcata tctttgtaac gcccttcaat cttgaaggag cgtacaccag
     9661 catcaatcag cgcgccgagg ttggcagtct gatcgttatc tttcatcgac agcagatgtt
     9721 tttcatagga aaccacccgc ccctgatcgt ctttcaatgt gtatggcaaa cggcacgcct
     9781 gcgagcaatc gccacggttg gcgctacgcc ctgtttgcgc atgagaaatg tagcactgac
     9841 ccgaataggc cacgcacagt gccccatgaa taaagaattc aatggtcgcg tccgtagcct
     9901 ggtgaatcgc gcggatctga tcaagattca gctctcgcgc cagcacaatc tgcgtgaagc
     9961 caacatcaga gaggaacttc gctttttcaa ctgtacgaat gtcgcactgc gtactggcgt
    10021 gcagttcaat cggcggaata tcaagttcca gaatccccat atcctgaaca atcagcgcat
    10081 cgacaccggt ctggtagagg tcagtaatca gccgttgcgc gggttccagc tcatcatcat
    10141 gcaaaatggt gttaagcgtg acgaaaattt ttgcaccata acgatgggca aacggcacca
    10201 gctcggcaat atctttcagg ctattactgg cattatgacg ggcaccaaaa ccagggccgc
    10261 cgatataaac agcatcggca ccgtgcaaaa tagcttcgcg ggcaatggcg gcatcgcgtg
    10321 ccgggcttaa cagttcaagt cgatgagaag atacggtcac attcttatcc agtagctgaa
    10381 aaatggcggc tattttagcc acaagcgcga cgttgcgaaa tagttttccc gcttaactgc
    10441 gcgggtaatg gatgagggaa tgaaaatgta cggtttgctc cccaccatta cgatagatat
    10501 gcgtgacgtc tgcagcaaat cgaacgcctt ccccacaatt aagggtctgc cattccccat
    10561 caacgcacag atcaagtttt ccatcaatga cgacgacatg ttcgattacc cccttctcat
    10621 ggggcgtcga ttcgcttatt gcaccagatg ccatctgaat cgagaagtgt tcaaagcaga
    10681 gttgtggatc ataaggaaac agcgaagtga tcaccatcgc ctgctgttgt ggatcataaa
    10741 ctgaaggcgt agcggactgc ggcggagaaa taaatgttga aaatggtacg ttcaacccgg
    10801 tggcaatttt ccataatgtc gcgaccgtcg gactggactc attacgctca atctgcccta
    10861 acattgcttt agaaacgccc gtcgcttctg ccagtcgcga aagactccag ccgcgctgct
    10921 ggcgtagttg tttaagtgtg gtggataaaa agcgagcgag attttccatg cgactcctcc
    10981 ggcaaaacgg aagtttatca cttgtgcgtt ataacggaca aatgctacgg tgcctgtacg
    11041 ctataacgca cgaggtgact atgcgtctgt tttctattcc tccacccacg ctactggcgg
    11101 ggtttctggc ggtattaatt ggctacgcca gttcagcggc aataatctgg caagcagcga
    11161 ttgtcgccgg agccaccact gcacaaatct ctggctggat gacggcgctg gggctggcaa
    11221 tgggcgtcag tacgctgact ctgacattat ggtatcgcgt acctgttctc accgcatggt
    11281 caacgcctgg cgcggctttg ttggtcaccg gattgcaggg actaacactt aacgaagcca
    11341 tcggcgtttt tattgtcacc aacgcgttaa tagtcctctg cggcataacg ggactctttg
    11401 ctcgtctgat gcgcattatt ccgcactcgc ttgcggcggc aatgcttgcc gggattttat
    11461 tacgctttgg tttacaggcg tttgccagtc tggacggtca atttacgttg tgtggaagta
    11521 tgttgctggt atggctggca accaaggccg ttgcgccgcg ctatgcggta attgccgcga
    11581 tgattattgg gatcgtgatc gttatcgcgc aaggtgacgt tgtcacaact gatgttgtct
    11641 ttaaacccgt tctccccact tatattaccc ctgatttttc gtttgctcac agcctgagcg
    11701 ttgcactccc cctttttctg gtgacgatgg catcgcaaaa cgcaccgggt atcgcagcaa
    11761 tgaaagcagc cgggtattcg gctcctgttt cgccattaat tgtatttact ggattgctgg
    11821 cactggtttt ttcccctttc ggcgtttatt ccgtcggtat tgcggcaatc accgcggcta
    11881 tttgccaaag cccggaagcg catccggata aagatcaacg ttggctggca gctgccgttg
    11941 caggcatttt ttatttgatc gcaggtctgt ttggtagtgc tattaccggg atgatggctg
    12001 ccctgcccgt aagttggatc cagatgctgg caggtctggc gctgttaagt accattagcg
    12061 gcagtttgta tcaggcgctg cataatgagc gtgagcgaga cgcggcggtg gtggcatttc
    12121 tggtaacggc aagtggattg acgctggtcg ggattggttc tgcgttctgg ggattaattg
    12181 ccggaggcgt ttgttacgtg gtgttgaatt taatcgctga cagaaaccga tattgacatc
    12241 ctccacgccc tgaaggacgt ggatccctgc tacgttcagg ctgtcgcctg aatcatttcg
    12301 gtgggttcct gcttcaacgg gcggcctgac tgcaccatct ctccacaggc aagaacggcg
    12361 tgccccgccg ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt atatccacat
    12421 acctggcatc tgaatttact ttgtgacagg cggttctctt tcgctgtatg accacagtac
    12481 gcgcaacgct ggcttgtata cgctggcgga acagcaagca cctgaccgcc acgccagagc
    12541 tgcttgtact caagctggcg gcgcatttca taccagccct gatccagtat cgaacggttt
    12601 aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc tgctgacttt
    12661 gacatgttgc tgaccttcaa atcctcaatg actatcattg cgtggttttt gctgacggcc
    12721 gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg cagtcgctgt
    12781 attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct taactgccgc
    12841 tgaagtctcg ccagcttctt ctggtttttc tgcaaactgt ttacaggctc aaagactgtg
    12901 ccatctgaca gcgtggcgag tttagccacg ccagcatcca gcccgaccat tgatgctgaa
    12961 gggtgaaccg gagtggatac ttcactttct gtctgaatac tgatgtacca cttaccgcag
    13021 gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctgtt ccggtagcgc
    13081 atccagccca gtttcggcag aaaaatacgg ctgttttcct gatcgagctt aacgccctgc
    13141 gggtagcgga atacatcatt ctgtccccgc tttttgaatc ggggaaaagc agcccgattc
    13201 tggaagaagt ttttgtaagc ccgctcaagg tctttcagtg actgttgcaa tggctgtgac
    13261 ggggcatctt taagccattg cgtttcagtg gcatttttcc actcaaccag ccaggaagcc
    13321 attttcccgt aagggatgta tttattaccg acctcatgat tctcattctg acgtgccaga
    13381 gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg ctcctgttga
    13441 ccaccgggtc ttaactggaa tttaaatgct tgtagtcgtt tcacaggcca aatgatactt
    13501 tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt cctgatgcat
    13561 gttcacctgg tctttgtcac cagataccga cgtcagattt ttgaccatga cgcgacagaa
    13621 aaactacgca cttacttttc aaatgtatgt gcttattttg aagctgaact ggttgaaatg
    13681 gatggcgaac cagatcacgt ccatttgtta attaactaac ctcccaaact ggcgatatcc
    13741 agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga tcgaccagat
    13801 attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt tgccagtagc
    13861 tgcggaggtg cgccaatatc cgtcatccgc caatacattg aataacagca aacacctggt
    13921 caggtggaaa accgcgcctt atatccccgc cctgaaggat ggggttttac ggcgcaccgg
    13981 ataaaaagcg ccatctaacg atggcgcgtc tggaactact ttttgttaac gtcaaacatg
    14041 gtggcatcgg tcgccatgtc atcaataact tgtttaatat tttcgaaagc cattggcgta
    14101 ctttcgttat tcaggtcttt accttcgccc tgacgaacga ctttgataac cggtttatta
    14161 gtcgctgcat caatcagctc accttcaaaa tagaggcgag tatccatggt acggtggcct
    14221 atagccattt gcgtccccgc aaccactaat gcaacaggaa ccacttcata gaattgcagc
    14281 ccttctttgc tggtatctac accggtaatg gccccacgga aaatcagact acgcggccca
    14341 gcggtggtaa ccactggttt acgctgcgct atcgcttctt tcatttcggt gttggtatag
    14401 ttcaaaattt tatccagaac tttctgccct acctgggtag aaggttttgg taccggataa
    14461 taagtgattg ggttccagac gatgctgtca tatgtgcttt gatcaaaact cgggtctacc
    14521 caacgtaaaa caggtttacc tgttgctgag gttgtttctt ttaaatcaga gtaattgttt
    14581 aaaaaaccag aatatttatc
//
LOCUS       DAZFAH010000108        14498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFAH010000108 DAZFAH010000000
VERSION     DAZFAH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.108"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..824
                     /gene="istB"
                     /locus_tag="ACHJWB_003080"
     CDS             78..824
                     /gene="istB"
                     /locus_tag="ACHJWB_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HFO4314667.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            930..1054
                     /locus_tag="ACHJWB_003081"
                     /pseudo
     CDS             930..1054
                     /locus_tag="ACHJWB_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1108..1413)
                     /locus_tag="ACHJWB_003082"
     CDS             complement(1108..1413)
                     /locus_tag="ACHJWB_003082"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFO4314668.1"
                     /translation="MRMVFIVMLDRHCKVYIACSSIINLVNCETKQRTLFERIYFSQY
                     WAKGDVIAKRAPISQWEPYSEESLLVIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            1369..1473
                     /locus_tag="ACHJWB_003083"
                     /pseudo
     CDS             1369..1473
                     /locus_tag="ACHJWB_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1510..1995)
                     /locus_tag="ACHJWB_003084"
                     /pseudo
     CDS             complement(1510..1995)
                     /locus_tag="ACHJWB_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            complement(2119..2439)
                     /locus_tag="ACHJWB_003085"
     CDS             complement(2119..2439)
                     /locus_tag="ACHJWB_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314669.1"
                     /translation="MDIWPEFQRDLEMYRDVVLSIKRNLRLYEECIESLVHQIGSTNF
                     DNAQPLFDDLFRMQSELATMLYKYEYKPGKRIQDLIYHLDRDDFYSRKYWHKKFSDGL
                     AWPE"
     gene            complement(2453..12322)
                     /gene="cdiA"
                     /locus_tag="ACHJWB_003086"
     CDS             complement(2453..12322)
                     /gene="cdiA"
                     /locus_tag="ACHJWB_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
                     /protein_id="HFO4314670.1"
                     /translation="MLQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFTQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQALSSGKKSVTHSDAAMTLNVTTVALDGENSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMAVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLHTAGTTQGQNLTVQADNWANSGSVLATGNLTASATGQLTSTGD
                     IMSQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNRGTVQGNHVTIRQNSVTNSGTLT
                     GIAALTLAARMDMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKQVLITADSL
                     ANSGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANS
                     LTSAGDITGVDALTLTVNQTLNNHASGKLLSAGVLTLKADSVKNDGQLQGNATTITAG
                     QLTNGGHLQGETLTLTASGGVNNRSGGVLMSRNALNVSTATLSNQGTIQGGGGVSLNA
                     TDRLQNDGKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVK
                     GTTLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLD
                     AQDFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLG
                     AGEAFTNNGMLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGS
                     LTMNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINT
                     SGNIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSY
                     GYFTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPA
                     GRIASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKT
                     FYGSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTS
                     SINNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELA
                     GALKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDS
                     HLFAGLYELLGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFD
                     TRYVSNAVLSRTGSRYLNGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLD
                     GSILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQ
                     NGLSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNIT
                     RRQQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGM
                     SAGNDINIAANLISGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTA
                     SSVSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAAVSTVTAGDNLLLVAGRD
                     VASQAAGVAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGG
                     DTTVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKT
                     THTISEDSASREAGSLLSGNRVTVNAGDNLTVEGSDVVADQDVSLAAGNHVDVLAATS
                     TDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSIT
                     AGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVGEKFEQKKSGLTVALSGTVGSA
                     INNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGDPNATGVSLSLTT
                     QKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDA
                     ANDVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGSEKGNGTE
                     WTETTIDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSV
                     AAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFSGDGGFDITVGNHTQLDGAV
                     IASTATADKNSLDTGTLGFSDIHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISA
                     GGHSGHAEGTTQAAVADGTITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRR
                     LQTVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPADATEEQRQAYLAKLRDTPE
                     YKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPELAHLLKSTEKDPAV
                     NAIAHAILGGTVAAMQGNNVAAGAAGAATGELAARAIAGMLYPGVKQSDLSEEQKQTI
                     STLATVSAGLAGGLTGNSTASAAVGAQSGKNAVENNYLSVSEKTELEIAKQTLKNSKD
                     PAEREKAQQKYDALLEKDIASDKEVIAACSNGNASSSACASARLKVIASKEGYEDGPY
                     NSKYSQQYADAYGQIVNLLDITSVDAQNQQQVKNAMINYFMVTKGVDRQTAESYTETT
                     QGLEIIAASVTPLIGQAASNKLSYLGIGKKISFDGDFYTVDGMKFSKSYYEKLWEQGR
                     PAPFVQAREVLNSNPKIEPDPRGAPGYLRYEGAGLEMIYNPKTGQVGHIQPVKVK"
     gene            complement(12335..14107)
                     /locus_tag="ACHJWB_003087"
     CDS             complement(12335..14107)
                     /locus_tag="ACHJWB_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4314671.1"
                     /translation="MQRRQNNILTITSLLSRALSVPCCDMFRRSSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPLVNQCLNITALTAVTDAMTDSYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVTALDGGWLHSDRDDPYSSGTLWGAAAGLSTTSGHVSGSFTAG
                     LPLVYPDWLAPDHLTVYWRVAVAF"
BASE COUNT         3132 a         4090 c         3650 g         3626 t
ORIGIN      
        1 aaaccagatc tgttatccac gatggaacac gaaaacgtac gcggcagtga gtattaccac
       61 tgatacggga tccaatgatg aatcatcttt acgaacaact gaccgcactt aaactcaccg
      121 gcttccgtga tgcgcttaaa aagcaacttg ctcagccggg cacataccag gagctgggct
      181 tcgaagaacg cctgtcatta ctgacagcag aagaactaac ctgccgtgaa aacaggaagg
      241 cagagcgtct gatcaaacat gcacggttca gacttaatgc tgagttatca aagctggatt
      301 atcgtaacaa tagagggctg gacagggccc tcatccgttc actcagtcag ggaaactggt
      361 taaccctgaa acaaaatatt ttactgaccg gggccaccgg cagcggtaaa acgttcctgg
      421 catgtgcact tggtcataat gcctgccgac agggatacaa ggtctactat tatcgcctta
      481 aagcgctgat ggaacagtgc tatcaggggc atgctgatgg aagatacagc aaacttttga
      541 ccaggctgaa taatagcgat ctgctgcttc tggatgactg ggggctggaa cctctctcat
      601 cagaacagcg tagcgacctg ctggaaatag tggatctgat gtaccaacga ggctcaatca
      661 tcgtagtgag ccagttgccg gtggaaaact ggtacaaaat gatcggagac tccacacatg
      721 cggatgccat cctagatcga ctggttcatg gcagtatcaa gatcgaactt aaaggagaat
      781 caatgcggaa aatacaatct ccgttgaccg aaggagatca gtgaaggtaa tttaaaaacg
      841 gttctgtgaa agtgacacga accgatctcc atcgatgtta ctcaccgatc tccttcacgg
      901 taatacgcaa ttcaggaggc cttttatgta tgatcaagaa ttttatcttc gataatctca
      961 taattttagc agcgccattt atgataaaaa atcccttaaa accaatctaa tctttttctt
     1021 cctctgtgta tttgttcccc gtatggcgtc ataatttatg catacgtgat gcgggatagt
     1081 tacaatatgc agcgatagcg ctaagtttta gttaaaatcc cccattagcg ggatatgggg
     1141 atctctggga ggttcaggtt ttagcatcgc gactttaata cgacagacgc tggtgacgat
     1201 gatgacaagc agtgactctt ccgaataagg ttcccactga ctaattggag cgcgcttcgc
     1261 aataacatct cccttcgccc agtattggga aaagtagata cgttcaaaca gtgtacgctg
     1321 tttcgtctca cagttcacga gattgatgat cgatgaacag gcaatataaa ccttgcagtg
     1381 gcgatcgagc attacaatga aaaccatccg catagtgcgt tgggttatcg ctctccgcga
     1441 gaatatcgac gtcagcgggt aatgttaact taagatacag cccctgtctg gaaataaggg
     1501 ggtaagaaca cccgcatcgg taactattat gactctggcc acagggttta cagcctcggc
     1561 aagggcatct gggaagtttt tttgtatctg cgagttctgc tgtttttctg acgggactac
     1621 ccagcttaac aggggaagcg agcatcaatc gcagagcagg ctggcgcgga gcacatgatg
     1681 ctcctgagat gaatatccac tccagtcaac ggcgatgaca caaagagata gctggctagt
     1741 cagcatggca ataatattat tgaaaatcag tgggatatcg cggtgaagag attcattacc
     1801 gagcagtcgg tcaacgcgtt tgattttatt tttgacccga gccaagccgg gtaaataacg
     1861 cccgatgctg gtcaacgtca gtgatgcgcc attgatgaga gcgatggtgg catccagcaa
     1921 agcattttgt cggtatttgt gaaacggtgc taacgcatcg tggaaaaaat tctgacatac
     1981 ttgtcgagca ggcatagaag cgatctcatt gaattggttg cacaaatcag tagatcacaa
     2041 aactctatgc ctgtcttttt tacccgccca ttactaggga ttcctcagcg ctaactgagg
     2101 gcgggatttc tacttctatt attcaggcca tgccaatcca tcactgaatt ttttatgcca
     2161 atattttctt gagtaaaaat catccctgtc cagatgatat attaaatctt gtatcctttt
     2221 gcctggctta tattcatatt tgtataacat agtagcaagt tcagattgca tacgaaagag
     2281 gtcatcaaat agaggctgcg cattatcaaa attggtgctt ccgatctgat gaactaacga
     2341 ttcaatacac tcctcataca accgaaggtt acgttttatt gataaaacaa catctcgata
     2401 catctcaaga tctcgttgaa attcaggcca aatatccatt tacttcctct ttttatttaa
     2461 ccttgactgg ttgaatatga ccaacttgtc ccgttttcgg gttatagatc atttcaagac
     2521 ctgcaccttc atatcttaag tagccaggtg cgcctctagg atccggctca atttttggat
     2581 tgctattcaa aacttctctt gcttgcacga atggcgcagg tctgccttgc tcccataatt
     2641 tctcataata acttttgctg aatttcattc catctacagt ataaaaatca ccatcaaagg
     2701 atatcttttt cccaatacca aggtagctta atttatttga tgcagcttgc cctataagag
     2761 gcgttaccga agccgcaatg atctccaagc cctgcgttgt ttcggtgtag ctttctgcgg
     2821 tctgacgatc cacccctttg gtgaccatga aatagttaat catggcgttt ttcacctgct
     2881 gctgattctg tgcatcaaca ctggtgatat cgaggagatt gactatctga ccatacgcat
     2941 cagcgtattg ctggctgtat ttcgagttat aagggccatc ttcgtaccct tctttcgacg
     3001 caataacttt cagtcttgcg ctggcacacg ctgaactact cgcattacca ttactacagg
     3061 cagcaataac ctccttatca ctggcaatat ctttttcaag caatgcatca tatttctgct
     3121 gtgccttttc acgctctgcc ggatctttac tgtttttaag cgtctgtttt gctatctcaa
     3181 gttctgtctt ttcagacacg ctcaggtagt tattctcaac agcattcttc ccgctctgtg
     3241 ctcccactgc tgctgatgcg gtgctgtttc cggttagacc gccggccagc cctgctgaca
     3301 cagttgccag cgtacttatc gtctgcttct gctcttcact gaggtctgac tgcttcactc
     3361 ccggatacag cattccggca atcgctctgg ctgccagctc tcccgttgct gcgcctgctg
     3421 caccagcagc aacattatta ccctgcattg ccgctactgt accgcccaga atggcgtgag
     3481 caatggcgtt tactgccggg tctttttctg ttgatttcag cagatgcgcc agttccggtg
     3541 cagaagctcc ggccagagca cccgctaaat taccacccgc cagtccctga agcgcggctg
     3601 tcgcggcctg tatacctcgc tgaatctcac tgccagtgcc atacttttcc tgttcctttt
     3661 tgtattccgg cgtatcacgc agttttgcca gatatgcctg ccgctgttct tccgtcgcat
     3721 ccgccggaac aggcccatat ttatcctgcg cagccttcaa tgcattcagt tccccctgcg
     3781 tccgcgcgat atctgccacc tgactgccaa tgtcactgat aagccccact gtctgcagac
     3841 gcctctgctc tttctccttg tcaaatatcg ggctgatact gtcattagcg tgcgcagggt
     3901 cacggctcag gttcgccaga ttctgcttct gattgtccct gtcccggatg gtgattgtgc
     3961 catctgccac tgcggcctga gtcgttcctt ccgcatgtcc gctgtgacct ccggcggata
     4021 tcatgccacc cggcatgtta ccctgaaatt tatccccgaa gctgccacca ccgctcagac
     4081 tgattccact gtgactgact ttataatccg cttcgttgtg aatatcgctg aagccgagcg
     4141 tcccggtatc gaggctgttt ttatctgccg tcgccgtgga agcgataacc gcaccatcga
     4201 gctgggtgtg gttgccgacc gtgatatcga agccgccatc tccggaaaac atcccggttt
     4261 gttcagcaac ggagtcaaag cggctcttca tcttatcccg ggaggcagcg atgtaacctg
     4321 agccggtcat ggagccaaag gtaaaactgc cgccggcagc cacgctggtc tgtttactgt
     4381 cgtacttact ggtgtcctgc tggctgctta tcagcaggtc gtggcccaca tcggcgataa
     4441 tcctgttgcc gttgacctga gcaccgttca gtaccgtatc ccgaccactg ttgatggtga
     4501 cggttttacc gctatctatt gtggtttcag tccactcagt accgttacct ttctcgctgc
     4561 cttttgccgc attaacgctg gcaaagacgc tgataccggc accgttacca ccggcaccga
     4621 tactgacacc cacgccaccg ccactgctgc tgttcctgcc cgttgttttt tgtgtgtttg
     4681 cagcgccact caacagaaca tcattcgcgg catccaggct tgtgttacca ccggccttaa
     4741 gctggcttcc tgcaatcaca atatctccgc ggttatctcc cctgtttttg ccggttgcga
     4801 caacagacag attattcccg gcattcagcg tactgccgga tactgtgtca ctttcagaat
     4861 gttgttgtga tttcgatttc tgggtggtaa gcgacaggct gactcccgtc gcattcgggt
     4921 caccggttgc ggtggccatt gccgcagcct gtccggcctg cacaccagac agcgctgtct
     4981 ttgtggcctg cagggctttc agacggctgt cactgctctc cttcgtctcc tgtgcactgg
     5041 tgaccgcatt attgatggca ctgcccaccg tgccggaaag ggcaaccgtc agcccgcttt
     5101 tcttctgctc aaatttttcg cccacagtac gacgatcatg ccccgggtca accaccacac
     5161 tgtcaccggt aatgctgata tcccggttcg caatcacatc cgaaccgctg atatgagcct
     5221 gtttgcccgc ggtaatactg acattaccgg cagtggagcc gatggtactg gcactctgac
     5281 tctgcgttgt cccggcctcg cggcggtcgt gcgttgtctt actgctgcca atggtgaaac
     5341 caataccgcc ggtacccatc agaccggatt tcttcgtttc cttaaagcgc caggacgtat
     5401 ctgtactggt ggcagcaaga acatcaacat ggttacccgc cgccagtgac acatcctggt
     5461 cagccaccac atccgaaccc tctaccgtca ggttatcacc ggcgttaacg gtcacgcggt
     5521 tccccgacag cagggagcct gcttcacggg aggcactgtc ctcactgatg gtgtgggtgg
     5581 ttttcttact gagaaaacct ccgctttttt tcttcgtttc cagatagtga tagtcacttt
     5641 ctgtcgccgt ggtcagggca acatcacgac cggcattcac gctgatattg ccggttgcgg
     5701 taacggatga cgcaacagcg gtgatatccc gtcctgcgtt gacggtggtg tcaccaccgc
     5761 tggcgatttc cgttccctgc tgacggactg tctcgttaat ctctttcttt ttcttcgacg
     5821 tatagctgtc gcctgcaccg gcagactctg ccaccaggtt cacatcacgt ccgccccgga
     5881 tgaccacgtt attttccgca gccacaccgg cagcctgact ggcaacatca cgaccggcaa
     5941 caaggaggag gttatcgccc gccgtcaccg tggacactgc tgcgtggctt tcatgacttt
     6001 ctgacctgcc gttgcgactg tttttgcttt ccctgactgc attcagactc aggtcgttac
     6061 ctgcagaaag cagggcgctg tgcccggcag aaacagagga tgccgtgaca tccagattat
     6121 ggcctgcagc catcgccagg ttaccgccgg cgctgatgct gctgccctgt gaggtggtgg
     6181 atgatgcact gttgtcatca gtgtgccaga aaccggactg acttttgctc ccgcttatca
     6241 ggtttgcggc aatgttgata tcattacccg cagacattcc aagggttcca ccggacgaga
     6301 ccgttgcccc ggtaatatca atgtttttcc ctgcatccag tgaaagtgaa tcagtgcctt
     6361 taatggtcgc aaccgggccg gtgtccgtac cgctgagatg cacaccacca tatcggctgt
     6421 cactgcccgc attccattgc tgacgccggg tgatattgct gatgttgcca ctcacgcttt
     6481 ccagttgtac ggttttaccg ctgatgactg agctgatatt gctgatatcc ccgatggcgc
     6541 tcaggtctag gctaccgccc gcgcttatca gccccgcatt caggttgtcg atatagccgg
     6601 tactgtcgag cgagaggccg ttttgtgcgt tgatgctgcc gccgctgttg gtgatattgc
     6661 cacccgcaag ctgcacgttg ttcccgctga taacgctgcc gttatgcagg gtgatatctt
     6721 ccggcgacag atacagtttc gggaccatga ctgtctgtcc gttgatggtg gctgactccc
     6781 accacagtat gctgccgtca agctgagcaa tctgttcagc tgtcagcgcc acaccaaact
     6841 ctaatcccag tcctttctgt tgtctggccg cgttatccat cagataccgc atctgttccg
     6901 tgtctgaacc cagtccgttg agataacgtg aacccgtccg gctcagcacc gcgttactga
     6961 cataccgggt atcaaagacc gcatccccca ggaaacgata atctttttcc ggtttcagcc
     7021 cgaggcggtc aagaaaatac gatgagccca gaaactgttt ttcatcggta tacgacggag
     7081 ccgtttcacg tggcgcctga cccggtttcg ctccaagaag ctcatacagt ccggcaaaca
     7141 aatggctgtc cacctgtccg agaccatcca gtttcgggtt caccgtaatc agatacggac
     7201 tgtccgggtc cgtggacgga accaggtatc cattgttgcc ggaaggcagt ggccagtcat
     7261 cactgatacc ggtctgaccg gtcagtggcg aacctccggc aatatttttc agggcacctg
     7321 ccagttcatc gtgccattgc ggagagccaa ccaccaccgg ctcatactgc tgcagcgctg
     7381 tctgtgtcag actgtctccg ccggtctgct gacttaacgt attcagtacc ggtgcagaga
     7441 ccaccggact gacactacct gcatgtgcag tggttgttcc gttattgata ctgctggtaa
     7501 aacgggtctt aacatccccg cccgcctgaa taacggaata atacgtctta ccgggcgtgt
     7561 aatctttttc ccggccatcc agtgaaaatc tgatggtatt gttttcaaat tccggtgaca
     7621 gcaggggcag tttatccaga gagcctgtgg catagctacc gtaaaacgtt ttcgggtcgt
     7681 agcggtatac cagatattca ttctctgtcc ccgtctgcca gctctgattg cttaactctc
     7741 tgcccgaaag tgcgatatcc ccattcgcca ggataaatga cgcccggttt tccagtcgtt
     7801 cagcctcagc agaaagatta cgccctgacg caatgcggcc tgccggatta tcagcaccgg
     7861 ttactgttgt gatgttctgg ctgctgagaa agcgctgtgt ggcactgtca gcaaacggag
     7921 cgtaataata aagcgtatcc attgtgatat tgcatgcccc gtgcccgttg cagggcgtac
     7981 cgtgctgatt ttcaacttca cgggtgaaat agccatagct gccgtcagga agaagggaaa
     8041 ggggaatatc aaccagagca tttcccattc cctgaatgga tgaggggtta gtccgggttg
     8101 ttgttgtggc agaaaatccc tcccgctggt tcagaagatg cccggtcctt acaacaatat
     8161 cgccctgatg cgtctcaata ttcccggagg tattgataat ctctgtgttt gcaccgccgg
     8221 aagcatcctt ctgtacccac agactgttgc cggccaggat atcaccatgc tggttatgca
     8281 gacggtctgt aaacagcttc aggttattcc ccgcataaat cagcgcactg ttcagcaggg
     8341 taccggccac attcattgtc agactgcctg ccgtgccggt aaaaccactg atggtgatat
     8401 cactccggct gttcagactc acatcgccac cggcctgaag tgaacccggt gcgttaagga
     8461 aaagacgctg tgcgctgaaa acactgttgc ctttaccggc agtcagcatt ccattgttgg
     8521 tgaatgcctc tccggcaccg agcaccatgg catcaccctg catcacaccg ccgttggtga
     8581 tggcattttg cgacgtgacg gaaagggttt tccctgcggc cagggtacca tgattcgtga
     8641 gggcagcaat cagtttcagt gtgacatcac cggtggccac cacctgaccc tgaccactga
     8701 agtcctgagc gtcaagcagc aggttgcctg cactgtacag ccgccctgta ccattttgca
     8761 gcagtgaact gcccttgacg ccaagcccgg aggttcccag cagggtaccg ctgttgctga
     8821 atgtgtggta attcaccagc aggtccgccc cctgaagcgt accggtatta ttcagcgtgg
     8881 ttcctttaac gtcggcactg ccggtggcaa gtacgcgtcc gccgttgaca gtattcacca
     8941 catccagcag cagggtggca gcctgtacca gtccgctgcc ggtgttcgcc agcacctgcg
     9001 ccgtcagcgt gaggttactg ccggagagga ttttgccgtc gttctgcaga cggtcagtgg
     9061 cgttcaggga aaccccgcca ccgccctgta tcgtgccctg gttactcagg gtcgcagtac
     9121 tgacattcag tgcattccgg ctcatcagaa caccaccgga acggttgttc acgccaccgg
     9181 aggctgtcag tgtcagcgtt tcgccctgca gatgcccgcc gtttgtgagt tgtcctgccg
     9241 tgatggtggt ggcatttccc tgtaattgcc cgtcgttttt gacactgtct gccttcagcg
     9301 tcagcacacc tgcactgagc agttttccgc tcgcgtgatt gttcagcgtc tgattcaccg
     9361 tgagcgtgag agcatccaca ccggtgatgt cacccgcact ggtcagtgag ttcgccttca
     9421 gggtcagatt ttttgcaatc cattgtccgc tgttgcttaa attcagtgca ctgagcgcca
     9481 tttcaccgtt cgaggtgact ttgctacctg ctgtgctgac aagctcaccc gtcagacgtg
     9541 cagtcaggct gtcagccgcc tggatcgccc cgctgtttgc cagactgtct gcggtgatca
     9601 gcacctgttt gccctgccag tgtccggaac tggtaatact gcctgcggtg attgtcagat
     9661 cgccgctggt cagcagtgaa cctccgttat tcatcagcgc aggttgaggg gatgccatat
     9721 ccatacgggc ggcaagcgtc agcgcggcta tcccggtgag cgtgccactg ttggtgacac
     9781 tgttctgacg aatcgtgaca tggttaccct ggacagtgcc gcggttatcc agtgaatttc
     9841 catcaagaga gagcgtgccg gccgaaagca gactgccccg gttgtccgtg gtggctgcgt
     9901 tcagcgtggt gtcaccctgg ctcatgatat cgccggtact ggtcaactga ccggttgccg
     9961 aagcagtaag gttaccggtt gccagcacgg agccactgtt cgcccagttg tccgcctgca
    10021 cggtgagatt ctgtccctgc gtggtccctg cggtatgcag tgttttaccc cggagggaga
    10081 ggtcgcccgc cgtcagccag cgtccgttac ggccctgtga gagggtgtcg cctgcaagcg
    10141 ccagtgcacc ggcgccctgc aacagaccat caccatccag cgtggtcgcc ctgacgttca
    10201 gcgtgtcagc gatgattttt cccggattgc tgagggaggc agtgtttaac gttaaaccat
    10261 tatcaccggt gataagcccg ctgttgcgga tgtccggtat atccagcgtc aggtctgcag
    10321 cactgtacag cgtgccgttc tgctgattat caagcctctg tgtgttaacg gtgagtgagg
    10381 cctccccctg caacagacca ctgttggtca gggtctgtga ctgtgtattc agggcggaac
    10441 caacaagcac accgctgctg gtcagttccg gcgcactgag gctgagcgac ggggcgctgc
    10501 ttttcccgct gtgggtgagc ttttcactgg cgttcaccgc catggtctgt tgtgctgcct
    10561 gcgtacctgc aagacgtgca tctctggcgt tgatgctgag atttttaccg ctctgaagct
    10621 gtgcgcccgc tgcggtactc agtttgtctg cctgaacccg gagggtgtca ccggcactgt
    10681 tttccccgtc cagcgccact gttgtcacat tcagcgtcat cgcagcatcg ctgtgggtga
    10741 ccgatttttt accggagctc agcgcctgcg cactgaccgt cagccctttg ccgccggaca
    10801 gcacaccgtt ctgtgtcaca tcctgcgcct tcagcaccag tacatcatcg ctcaccagcg
    10861 aacctgtact ggtcagtttc ccactggccg tgatatccac tttgcccttc gcgccagtgc
    10921 ggccgctctg ggtaaagtcg cgggtattca cggtcagggg accgccactg agcagggagc
    10981 cactgttgct gagcgttgta ctgccgagcg tcagggaagc cccctgaaca gcaccactgt
    11041 tattcagcgt gccggcatcg agtcccgcat gacctttcgc cagcaatatt ccgtcctgtg
    11101 tcagcgtggt ggcgctggcc gtgagattct gcccggcggt tatctgtccc tgtgttgtca
    11161 gcgtgtcact ggcgacagtc acgatatcgc gggccgcgtt aatctggctg gcggtatcct
    11221 gtgtgatgtt tttcgcggca agcgttacat cccggccggc agtcagtttt tcattctgtt
    11281 gagtgattct gccgccggcg gtcaggctga ggtccttgtc gctgttaagc gttccattgc
    11341 tgagaacgat atcgctccgg ctgctgacgc tcaggttacc tcccgcttta tggtcgccgg
    11401 ttaaggtgac gccctgacct tttgcagtga cggcccccgt ggcgagactg ttgttgacag
    11461 tcagtctgcc gctggcatcc agggtgatat cgccatcgcg ggcataaagg ttaccaagat
    11521 taaccccgac accactttca gtggaggtca gatgaatacg cctggcgtac attccaccga
    11581 gcgcgccggt atcaacggca actttcggca catcaccttc gcccttcagg gcactgacgc
    11641 gaccatctgc agttatccgg ttagcgcctg cagtgacagt taaatccttc gcatgaagcg
    11701 cggcattcac ttccgttgca cgggcaataa tggatacggc atcgctccgg gtgccgtcca
    11761 ggcccgcgcc attgatggtg atactgcctt cagtcacctc cagcgcctgc aggctgccgt
    11821 cggcattcat cacaggtctg cctgtggtga gcgtcgcgtg cggcgtgttg ataaaaccac
    11881 agccgtcaca ggtgatacca tacgggttgg caaccatcac attcgccgct ttgccggcca
    11941 cttccgtata gccctgcagc agtgaacggt taccgccggt cacttcgttg atgataccct
    12001 tcgcttcccc gcccgctttc aggttcgggt tattctgtat cagtccacca agctgcgtcg
    12061 gattaagctt accggtggca ttattgagaa tcagcccttc cttcccgacg ttgtaatccg
    12121 taaaccggtt atgcgaaatc ccggccccgt tcggcgtggc aatgttcacg accggcacac
    12181 catttgccgc tttatccatt ccggccccgt tttgtggggt gatgacggcc cccacagccg
    12241 gtaacaacgg ctgcccggcg ataatcgtac tgataaggta actcagcagg cggtaagtga
    12301 agcgaacggg aggctgaagc atggtaataa tcccttaaaa cgcgacggca acgcgccagt
    12361 aaaccgtgag atggtcaggg gcaagccagt ccgggtaaac aagaggcagt ccggcagtga
    12421 acgaaccgga aacatggcca ctggtggtgc tgagcccggc agcagcaccc cacagcgtgc
    12481 cggacgagta cgggtcatct ctgtcagagt gcagccagcc accgtccagt gcagtcactg
    12541 cacggacagt tcccacatat ggcagggaga acagagacca ggacagctca tttcgcagat
    12601 aaccaccgtt attaccggag atatactgct ccttaaagcc acgcactgaa ctctcgcccc
    12661 cgaggctcag ttgttccaca ccatgaagac ggtccggtga ccactgggca taagcgctgg
    12721 tcagccacca caccctgtcc gtgacggggc gctgaaaact ggcactcacc gaccatttcc
    12781 ggaactgatt tacgggcagg tctccccttt tcccgtggtc gctttctgcg ccgaaccagg
    12841 gcatcccccg tgtgaatacc ggattcagtg ttccgacacc ccccagaaac ttgtgtgtgt
    12901 gattcagccc gacagaaaat gaagtgagtt tacggctgct gccctgaagc agaacatcat
    12961 ccagataatt gtgaataatg cggtgctgca gacctccggt cagtgctgtc ttcatgtccc
    13021 cgttacggaa caggacatgc gacagtccca gccggtgagt ctgcaggtct cccgtggaac
    13081 gccaccgcca gccccggtta tcaatggtgc tgagatagtc actccatgaa tacgtgtaat
    13141 ccaccagggt atagccatac ggcagactga caccggcggc aaaattcctc gcatcatgtg
    13201 acaccgaaaa gtcactgctc cgtcccccgc tgacaaacca gttgtcagcc agccccagag
    13261 gattattaaa ggaaaggaca ccatttaact gccccgtacc ggtattcttc tgcccgctgt
    13321 tgtcgatgcc cacgctccct gtgacaggcc attccggcaa tgccgtcagt gtcaccaccg
    13381 accatccctc acggtcaccg ggcgatattt caatctgtac cggctccgta cgcagacgat
    13441 taatctgctc catcccctgc tcaatatccc gcaggttcag aaccttcccc tccattcccg
    13501 ggaaaaccat cttcagggtg cgggcaggaa ggtcagcgcc ttccgcccgg atttgctgca
    13561 gcctgccttc catgaccgtt atgtgcagta cgccccctga aaggtcctgc tctgtcagaa
    13621 aggcccggct ggtgatatat ccccggcgta tatagctgtc cgtcatggca tccgtgaccg
    13681 cagtcagagc ggtgatattc agacactgat tcaccaacgg tgccaccagt ctgtcggttt
    13741 ctgcagacgt cagtcgggtg gccccactga caacaatgcg tgacaccgta aagcagggac
    13801 cttcagtacc ggcagacgtt tccggagacg gcgtgatggt cagcggcgca ctgcgctcca
    13861 gcgcatcacg ctgacgctgg ttttcatcca gcaactgctg ctgttgctgc tgaattgcac
    13921 tgcggtcacc cggagacagc atggctgccg gagacgaaaa tgcggggata aaaaaaccag
    13981 aaaaaacgga gagggagaga tagcatatcc acggagagct gcgccggaac atatcacaac
    14041 aggggacaga caacgcacgg gataaaaggg acgttatcgt cagaatgttg ttctgccggc
    14101 gctgcatgtt ctccccgtat tctggcctgt gtgatgccag tgtaaattca tgtcaaaaag
    14161 tgtcagcgag aaaatttaca cctgaaaaag tctgtcatga aacagtttta tgacaagaca
    14221 tcaaaaaaat gtcaaaaacg tgatctttct tcagcttttc agaatattga gtaccaggaa
    14281 gtcaatatca attctgggac acatatgcat ttaccattaa aaccgggcac aacaagctgt
    14341 tcagtctcaa tccatacccc gtgttttatc aatatcagaa taagaatatc tgcggataaa
    14401 taaaaaagga atgtcgaggg agcttaccgc agagggctct ccacctcaca aaccatttac
    14461 aaaaatgata ccggcatatc tttggtccct gtcacaac
//
LOCUS       DAZFAH010000109        14421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFAH010000109 DAZFAH010000000
VERSION     DAZFAH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.109"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..799)
                     /gene="artM"
                     /locus_tag="ACHJWB_003088"
     CDS             complement(131..799)
                     /gene="artM"
                     /locus_tag="ACHJWB_003088"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4314672.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(799..1515)
                     /gene="artQ"
                     /locus_tag="ACHJWB_003089"
     CDS             complement(799..1515)
                     /gene="artQ"
                     /locus_tag="ACHJWB_003089"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4314673.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1522..2253)
                     /gene="artI"
                     /locus_tag="ACHJWB_003090"
     CDS             complement(1522..2253)
                     /gene="artI"
                     /locus_tag="ACHJWB_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4314674.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2271..2999)
                     /gene="artP"
                     /locus_tag="ACHJWB_003091"
     CDS             complement(2271..2999)
                     /gene="artP"
                     /locus_tag="ACHJWB_003091"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4314675.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3217..3732)
                     /gene="ybjP"
                     /locus_tag="ACHJWB_003092"
     CDS             complement(3217..3732)
                     /gene="ybjP"
                     /locus_tag="ACHJWB_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4314676.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3858..4181
                     /gene="ybjQ"
                     /locus_tag="ACHJWB_003093"
     CDS             3858..4181
                     /gene="ybjQ"
                     /locus_tag="ACHJWB_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4314677.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4178..5008
                     /gene="amiD"
                     /locus_tag="ACHJWB_003094"
     CDS             4178..5008
                     /gene="amiD"
                     /locus_tag="ACHJWB_003094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4314678.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5005..6018)
                     /gene="ybjS"
                     /locus_tag="ACHJWB_003095"
     CDS             complement(5005..6018)
                     /gene="ybjS"
                     /locus_tag="ACHJWB_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4314679.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6117..7547)
                     /gene="ybjT"
                     /locus_tag="ACHJWB_003096"
     CDS             complement(6117..7547)
                     /gene="ybjT"
                     /locus_tag="ACHJWB_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4314680.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7558..8559)
                     /gene="ltaE"
                     /locus_tag="ACHJWB_003097"
     CDS             complement(7558..8559)
                     /gene="ltaE"
                     /locus_tag="ACHJWB_003097"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4314681.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGTDVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8596..10314)
                     /gene="poxB"
                     /locus_tag="ACHJWB_003098"
     CDS             complement(8596..10314)
                     /gene="poxB"
                     /locus_tag="ACHJWB_003098"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4314682.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10447..11415)
                     /gene="hcr"
                     /locus_tag="ACHJWB_003099"
     CDS             complement(10447..11415)
                     /gene="hcr"
                     /locus_tag="ACHJWB_003099"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4314683.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLFLA"
     gene            complement(11427..13079)
                     /gene="hcp"
                     /locus_tag="ACHJWB_003100"
                     /gene_synonym="priS"
     CDS             complement(11427..13079)
                     /gene="hcp"
                     /locus_tag="ACHJWB_003100"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4314684.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMANLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVTATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13223..14122)
                     /gene="lysO"
                     /locus_tag="ACHJWB_003101"
     CDS             complement(13223..14122)
                     /gene="lysO"
                     /locus_tag="ACHJWB_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4314685.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLNNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3482 a         3849 c         3821 g         3269 t
ORIGIN      
        1 atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca ggcaccgtgc
       61 tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc ggcgaatgta
      121 cgcagctaca ttaatttcgc cgttcgaatg ccagtgcttt gcgctcgatc agacgcatca
      181 tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg aacaccatta
      241 catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc agcgtaatgg
      301 tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa gaagagagcg
      361 agcgtttaaa ggcatacggc agcaggatcg ccagcgtatc ttttttgctc attcccaggg
      421 cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa aacagctgcg
      481 tggtatacgc cgcactgttc agcgataatg caatcagcgc acataaccac ggttctgaca
      541 acaaatgcca cagtgccgga tactcctgca aagtcggaaa ctggcccggc ccgtaataaa
      601 tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa ccccgcacca
      661 gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat gccagaatca
      721 gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct ttcatcagtt
      781 cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc gcaggtcaat
      841 gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg ccgccacaat
      901 gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca gcattaaatc
      961 attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc actggttacc
     1021 gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa agatagccga
     1081 tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg ctttcaacgc
     1141 gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga tgacaccaca
     1201 aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct gcacgaaccc
     1261 aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc caaaatagat
     1321 aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg ctgaacctgc
     1381 ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg ccagcgccag
     1441 cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc ttgctaaagg
     1501 aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg tttcgtaagt
     1561 gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct cagtgttgcc
     1621 ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca ctttgtcgcc
     1681 caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg tgtcaccgaa
     1741 gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc tgtcatacgg
     1801 aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg tcccgttctg
     1861 tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt gctgacccac
     1921 aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt cacgctccgg
     1981 agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc ttgggatcag
     2041 gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac acagcgcttg
     2101 tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt caaacggagg
     2161 ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa gactaaaacc
     2221 tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc ttagtgagag
     2281 agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc gccttgttct
     2341 acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc cacttcaact
     2401 tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat gatgctgacg
     2461 atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag cagtacctgc
     2521 ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc agaaagatgc
     2581 agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt ttctgcacgg
     2641 gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat caggttttgc
     2701 tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac gttacgacgc
     2761 aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt gcctgcaatg
     2821 ttgagcgtac cggagcgcgg catctcaagc agattgagta cacgcagcag cgagctttta
     2881 cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc cagcgtgata
     2941 tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg aatactcatt
     3001 gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc tttgacagaa
     3061 taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc aataaacata
     3121 gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct cacgaaatgc
     3181 cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga tggactgacg
     3241 gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgtacatcgt cgatcaccca
     3301 acactggcct tcctgaatca ttaacacttc atcctgccag ctctgatcac cctgttttag
     3361 atccacacgc agaggaatat ttcgcgcatc acgattcggg attgttgagg cactggcaac
     3421 acgggcgctc tctggcaagg ttgtgcgact ggagaatgga tcgttggtca gtagttcccg
     3481 atggttatta tcccgggagg catcgctaag cagtgtcgcc agtttgtcgc tcagataagg
     3541 gcgcaaggcg gtgatgtcgt tgctgcggtg caaaatgcgg tagtcataaa attgctgggc
     3601 cacgttatcc gggcctcctt caacgcaagg accactgcgt gtgccgttat ctttataagc
     3661 tggagtgact gtggtgcagg cactgaggag cagtgcgcag gggataagca ttgtcaattt
     3721 gctgtagcgc ataatgattt ccttataagc gatcgctctg aaagcgttct acgataataa
     3781 tgatatcctt tcaataatag cgtatcagtc tgataatgct ttagagatcg aaggcttaac
     3841 aaacaaggag atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg
     3901 ttgaatattg cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt
     3961 tctttgccgg tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc
     4021 gtaaagcacg ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg
     4081 atgccgtcgt cggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga
     4141 tggttagcgt cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctgat
     4201 cgctgccgct ctgttactgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg
     4261 atatcagctt gatacccgac gccaggcgca ggcggcgtat ccgcgcatta aagtgctggt
     4321 gatccactac accgcagatg attttgatag ctcgctggcg acactgaccg ataagcaggt
     4381 cagctcgcat tatctggtcc ctgcggtacc accgcgatac aacggtaaac cgcgcatctg
     4441 gcaactggtg ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc
     4501 aacgcgcctt aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc
     4561 ggccggagtg aaatattttg ccccgtttga accggcacag attcaggcgc ttattccact
     4621 ggcgaaagat attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga
     4681 tatcgcaccg cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca
     4741 gcaggggatt ggtgcctggc cggatgcgca gcgggttaac ttttaccttg ccgggcgcgc
     4801 gccgcacaca ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga
     4861 cgttaaacct gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca
     4921 tttccgcccg acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc
     4981 attgctggag aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc
     5041 ggcagttttc tcgatacctt catccagggt gatgaccggc tgataaccta actcttcctg
     5101 cgcacgcgta atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag
     5161 cggcggctct tttgctgact tgcggcctaa acgctccatg ctgcgggcga tcatatccag
     5221 catcgggtag gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg
     5281 cacgatgctg cgcagtgtgc gatgctcgcc gttggtgatg ttgtacacac gcccggaagg
     5341 tagcttatcg caggcttcct ggcttgccag ccacattgcg tgcacggcat tttcatagta
     5401 ggtcatatcg accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat
     5461 catatgcgcc agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg
     5521 cagaatagta aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct
     5581 ggctgctttg ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat
     5641 atcgcgatgg tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg
     5701 cacaccccag gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc
     5761 gaaagcctgt tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat
     5821 gcccgcgagc atcactttag cttgtgatga aaccagctcg gtcagatccg ccggaacaaa
     5881 ctctgcgccc attttttcca gcaatttgcc cattgcctcg ttgcgaccgg tcgctcgcac
     5941 gctgatgcct ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc
     6001 ggtaaccagt accttcatat caatccactg ttgttgagaa aataacgtgc gcattcttcc
     6061 gtgatttccc ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa
     6121 tctgtgcttt gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga
     6181 tgcgcgggga tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa
     6241 gcgcggacat cgatagtacg atagtcgcct ttatcttcca ggctaaaaca cagtcgtccc
     6301 agccccggcg ctttcatgcc aaataacaac gtaagttgtt tttccggttc aacgacaatc
     6361 actttccagc tatctaccgc atcgccagtc tgtaaatatt cgcgctccgg gcggcctttc
     6421 gccagcttat gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca
     6481 aagaaataac gctctttacc gccgatttgg ttcactacct gccataaagc agcaaggctg
     6541 gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc cggtcgccag
     6601 cgggcaaagg cctgagcgtc gtagccccag tcgctggagt tgaccagttt ttcctcctct
     6661 ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt
     6721 agcgcggtat catccgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg
     6781 gtgggcggta cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg
     6841 gggatgggga tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc
     6901 tgataactga gcacctctgg tccggcggct tcgaagatgc ggtgttcgct ggccggatga
     6961 tctaacaacg ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa
     7021 cgtacccagc gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg
     7081 aacgccgctg aacctgcgcc aacgataatt ccggcccgaa gttcggtcac aggtacattc
     7141 gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca gatgatccga ctgctcatgt
     7201 ggcggggcct gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca
     7261 tcgcggacgt tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg
     7321 ctgtgcacca gaaaatagac ggtatcgata tcctgcaaca gggccggaag gttatccggc
     7381 cagctgagat cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg
     7441 acatgacgtg ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc
     7501 tgaccaatgt agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta
     7561 acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc
     7621 aagatgcgtc accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat
     7681 gtattcgcct aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc
     7741 ctgacgcatc acatccgtgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc
     7801 gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag
     7861 aatcccggac tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat
     7921 gtaatcacga ttaccgacga gtaatgaacc gactggcgtc ccaagacctt tcgacaggca
     7981 aatggtgaac gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac
     8041 ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga ttgcgctcgc gggtaaattc
     8101 ccatgcttct ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttccagact
     8161 gagtaatttg gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc
     8221 cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc ggctgaatac tgcccagcac
     8281 cgctgcgcca ccggcttcaa acagatagtt atgcgcggcc tgaccgacaa tatactcttc
     8341 gccacgttcg cagtgactga gcagagcgac caggttggcc tgagtgccgg taggcagaaa
     8401 aatggcggct tctttaccgg aaagctctgc tgcatagtcc tgcagagcat taacggtagg
     8461 gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc atcgcttcga gcatggcgcg
     8521 gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg
     8581 gaaatgccac cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc
     8641 gcgtccgctg atgattgcgc gcaacatata caagctaaat cctttggcct gttcgagttt
     8701 gatctgcggt ggaatggcta actcttcttt ggcgaccacc acatccacca gcaccggacc
     8761 gtcgatggag aaggcgcgtt gcagggcttc atcaacttca gacgcttttt ctacacggat
     8821 acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta gttcggtgcc
     8881 gtcagtcaaa tagccaccag ctttcatctc catcgccaca aagcccagca cgctgttgtt
     8941 aaagacgaca attttcactg gcagtttcat ctgcactact gagaggaaat cgcccatcaa
     9001 catgctaaaa ccgccatcgc cgcacatggc gaccacctga cgttctggct ctgtcgcctg
     9061 cgcacccagc gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag
     9121 gcgacgcttg ccgttcattt ttagataacg tgccgcccac accgttggcg taccaacgtc
     9181 acaggtgaaa atagcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg
     9241 gtgaatggct ttctcgctcg gtttagctaa atcgtccagc cctttgcggg cgtcgcggta
     9301 atcttccagc gctttatcca gaaacttgcg atcggctttt tcttccacca atggaagcaa
     9361 tgcacgcaga gtcgacttga tatcgccgac cagtgccata tccaccttgc tgtgagcgcc
     9421 gatgctggct gggttgatat caatctgaat gattttggca tcggtcgggt agaaggcgcg
     9481 gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc
     9541 tgacgagaag ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg
     9601 ttctttaccg cgcagggcat gaacaatagg cgctttaatt ttcccggcaa actcaactaa
     9661 ctctttatgc gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg
     9721 cagcagttgc gccagtttgc gtaactcttc ttcttccggc gtcacgactg gttgtggcgc
     9781 atgataccag tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcctggtaa
     9841 cacgacaacc gaaacgccac ggttaagcac cgctttgcgc atggcaatcg ccagtacttg
     9901 tgggatctgc tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc
     9961 ttgtgggtgg gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc
    10021 aatcgccagt accggaacgt gattgcggtg gcaatcgaac aggccgttga ttaagtgcag
    10081 gttgccgggg ccgcacgatc cggcgcagac cgccagttct ccgctaagtt gtgcttcagc
    10141 gccagcggca aaggccgcca cttcttcgtg gcgggtggac atccactcga tggtgcccat
    10201 gcgattaaga ctgtcactaa gaccgttcag agaatcgcct gtgactcccc agatgcgttt
    10261 cacgcctgcc gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct
    10321 ccatctcctg aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat
    10381 gggagagaaa ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata
    10441 aggcgattat gcgagaaaca aatccccctg cggatggcag gagcaggcca gtacgtaacc
    10501 ttcagcgatt tcggcgtcgg tcagcgtcat tgtgctgctc accgtatatt caccggaaac
    10561 cacttttgtc ttacagcagc cgcaaacacc cgcacggcag gcggcgacaa ccggaacgtt
    10621 attgctttcc agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg
    10681 ttgcagtttg gtgaatttca gaccgctggt cgctgcttcc gctactgggg tgaagaattt
    10741 ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata
    10801 cggagccggg ccgcaggtca tcacggtacg tgaagctaag tcaggaacgc gagtcagcag
    10861 ttcgcgagtg agacgaccag cgataaagcc ttcggtaacg ttattttccg ccaccagcgt
    10921 taccggatag ttacgccact catcggcgaa aataacatcc tgcggcgtgc gcacgttgta
    10981 gatcacctgt acatcggcct gtggacggtt cttcgcaagc cagcgacgca tcgacataat
    11041 cggcgtgacg ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca
    11101 ggtaaattcc cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt
    11161 cagccactgg gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact
    11221 cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct
    11281 gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca gggaaatcgt
    11341 ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt
    11401 cgttggcatc gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca
    11461 acagtggtga tagaacgcag gccaaatttc tcgttcagca ccgccagcag gtctggtgtc
    11521 aggaaaccag gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc
    11581 agcagaatga cgatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg
    11641 ttgacaccgc agcccagttt ctctgccaga gtgacagcca gaataatcgc tgagtaagca
    11701 tcgttacatt gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt
    11761 ttgttaaagc gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg
    11821 gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg
    11881 aagatatgac gcagtttttc acggctcacc agatcaatca gcgtatcagc agcgccaagc
    11941 agcgtctggc gaccaaaacc cacggtgata aggtgcggaa tttcgctgta cgggaagcct
    12001 gccatctgtt gcgcctgggt gataaccgca gagaaatcat caccgtccag atgacgcacg
    12061 ccaggccagc caacaatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg
    12121 tcgatgatgc agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc
    12181 tgattctgcc agccactgcc gtagttaccg accagatgct tgaatttacg cagctccggg
    12241 tagccatgcg caggcagcat ttcgccgtgg gtgtagacat taacgcccgt gccttcggtc
    12301 tgttccagca ggttgtagag atctttgaga tcgtgaccgg aaatcagaat gcatttaccc
    12361 gccgtcgctg tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct
    12421 gcatccagaa tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga
    12481 agcgcgttca tatcggcagg ccaggtcccc agccacgcca tgattttatg atactgggca
    12541 taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct
    12601 ttcaggccat acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta
    12661 tctttgttag gggtaaattc tgctgcctga cgttgcagct cgccgagatc atcgctcacc
    12721 agttgcaggt tagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgt
    12781 gctttcagcg cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat acgcggagaa
    12841 tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg
    12901 tggttgatga tgccgtattc acgcgctttt accgcccagg cagaaagccc ttgcagcgcc
    12961 gcgatgagta aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg
    13021 tatgagcagc cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg
    13081 atcacacctt ttaaagttat atttaatata catgtttaag gttaagacgc ttaacgcggg
    13141 gataaaaggg atttttcatg caactttaag ggagattgat ttagcgcaat tttggcggca
    13201 gggatctacc gccagagagg tattacgcag agaaaaaagc gatgaggatc ggcactaaca
    13261 ggctaagaat aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt
    13321 gaagaacggg cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc
    13381 ggctgcggcg aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat
    13441 taaaaaacgc cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa
    13501 gagaatacca gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa
    13561 taaaggcgtt aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca
    13621 ttccccggcg attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac
    13681 caacgaggaa aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga
    13741 aagccagtcc actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg
    13801 actccagcgc catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg
    13861 gcaggcctcg ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac
    13921 tgacggcaga ataatgcaga atcgccaaca ggttactggc gaggttattg agaaacgcca
    13981 gactgatacc cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa
    14041 cttttaacgc agcttgttgg cgaagcggaa tgaggtaacc cacaatcagg ggaaccagaa
    14101 tgattaacag cccagaaaac atgaaaaccc agtccttgca aagatgaagt cgaaatgcgc
    14161 gatgacacac tactgaaagc ggaaggacga gtaaagttgc aattaaaaag aaatgttatg
    14221 cataaggatc ggtagggtat tcattttcat ttaaagatat tcttgcgctt taattacaaa
    14281 ccgcaccggt gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc
    14341 acatggatgc gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag
    14401 ccgtaaggga taagtcgagg g
//
LOCUS       DAZFAH010000110        14272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFAH010000110 DAZFAH010000000
VERSION     DAZFAH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.110"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..417
                     /locus_tag="ACHJWB_003102"
     CDS             61..417
                     /locus_tag="ACHJWB_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4314686.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFCSN"
     gene            complement(468..1364)
                     /gene="yhaJ"
                     /locus_tag="ACHJWB_003103"
     CDS             complement(468..1364)
                     /gene="yhaJ"
                     /locus_tag="ACHJWB_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4314687.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1469..2170
                     /locus_tag="ACHJWB_003104"
     CDS             1469..2170
                     /locus_tag="ACHJWB_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4314688.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2193..2357
                     /gene="yhaL"
                     /locus_tag="ACHJWB_003105"
     CDS             2193..2357
                     /gene="yhaL"
                     /locus_tag="ACHJWB_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4314689.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2569..3879)
                     /gene="cyuA"
                     /locus_tag="ACHJWB_003106"
     CDS             complement(2569..3879)
                     /gene="cyuA"
                     /locus_tag="ACHJWB_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4314690.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNAKAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3907..5238)
                     /locus_tag="ACHJWB_003107"
     CDS             complement(3907..5238)
                     /locus_tag="ACHJWB_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4314691.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5513..6877)
                     /gene="tdcG"
                     /locus_tag="ACHJWB_003108"
     CDS             complement(5513..6877)
                     /gene="tdcG"
                     /locus_tag="ACHJWB_003108"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4314692.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGLIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6949..7338)
                     /locus_tag="ACHJWB_003109"
     CDS             complement(6949..7338)
                     /locus_tag="ACHJWB_003109"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4314693.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7352..9646)
                     /gene="tdcE"
                     /locus_tag="ACHJWB_003110"
     CDS             complement(7352..9646)
                     /gene="tdcE"
                     /locus_tag="ACHJWB_003110"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4314694.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9680..10831)
                     /gene="tdcD"
                     /locus_tag="ACHJWB_003111"
     CDS             complement(9680..10831)
                     /gene="tdcD"
                     /locus_tag="ACHJWB_003111"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4314695.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLVMEHLAVLGVEIDTEM
                     NNRSNSFGERIVSSENAHVICAVIPTNEEKMIALDAIHLGKVNAPAEFA"
     gene            complement(10857..12188)
                     /gene="tdcC"
                     /locus_tag="ACHJWB_003112"
     CDS             complement(10857..12188)
                     /gene="tdcC"
                     /locus_tag="ACHJWB_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4314696.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12210..13199)
                     /gene="tdcB"
                     /locus_tag="ACHJWB_003113"
     CDS             complement(12210..13199)
                     /gene="tdcB"
                     /locus_tag="ACHJWB_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4314697.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13298..14236)
                     /gene="tdcA"
                     /locus_tag="ACHJWB_003114"
     CDS             complement(13298..14236)
                     /gene="tdcA"
                     /locus_tag="ACHJWB_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4314698.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3685 a         3835 c         3342 g         3410 t
ORIGIN      
        1 atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag ggatgttttt
       61 atgcagtggt atttagccgt attaaaaaat tatgttggtt tctctggtcg cgcacgtcgt
      121 aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat caatgtcatt
      181 caattgattt taggtttaga gtttccattc ctttctctca tttacctggc agcaacaatc
      241 attccggtca ttgctctgtg tgttcgtcgt ctgcacgata ctgaccgttc aggtgcgtgg
      301 gcgcttctct atctggtacc gatcattggc tggttagttc tgtttgtatt tgcctgttta
      361 gaaggcaatt ctggcagcaa tcgttacgga aacgatccta agttttgttc aaattaatct
      421 tagaattggg gcgacatttc gccccttttt attaacaata acaaatatta ttttccgcta
      481 aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact gtcacgacgc
      541 caggccataa taatatcgat ctcgctggtc gattccgggc tgacgacacg caaccgccct
      601 tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag ccccgccagt
      661 aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt gtccagcagc
      721 tgtacggtca acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc acgatatttc
      781 acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc aggcgcggca
      841 acgtagacgt tcattaaggt atagagcttg cgcgagttga tctccgacga ggaacgaaaa
      901 tgcatatccg gcgcgataac gatatccgcc cgcccctgct ccagtcgttc ccacgccccc
      961 gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag tttgtcgatt
     1021 aacgggaaaa aggcaggtgt cggtaccagc gcctcggtca caatggtgag atgcgtttcc
     1081 caaccgcgcg cgagggcttc cgcatcggta gtcagtttat ctgctgcttc cagcaaaacg
     1141 cgccctcgct ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg
     1201 tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct aagtgcggaa
     1261 ggcacgcgtc ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc gatcgcatcc
     1321 ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg
     1381 aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc cccttaccat
     1441 aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca ggcagtgtgg
     1501 acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac actacttcga
     1561 cccgaaattg ttaggctatg cctccctgcg tgtgcttaac caggaagtgc tggccccagg
     1621 tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga ttctggatgg
     1681 agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt
     1741 gctgctctct acccagccgg gtgtcagcta cagcgaacac aatctcagca aagacaaacc
     1801 gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc cgctgattca
     1861 aaaactggcg cttaatatgg gcaagcagca attaatcgcc tcgccagagg gtacgatggg
     1921 aagcctgcaa ttacgccagc aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag
     1981 tgcgaatttc cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca
     2041 tgcgcttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg cgtttattcg
     2101 tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc tgatagattt
     2161 gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc
     2221 agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg
     2281 aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg
     2341 atgaagctac cgcgtaaaga caggttgccg gatgaggcga aaacgcctta tccggcctac
     2401 aaaatccaac aacattaacg aattaaacaa cccgtaggcc ggataagatg cgccagcatc
     2461 gcatccggca tcaggtacaa aacacaacaa catcaacaag ttgagcaccc cgtaggccgg
     2521 ataaggtgcg ccagcatcgc atccggcaca atactcaaaa cctggcgttt atctggcctt
     2581 gctcgccata atctcgataa tctgccgatc cgtttgctgc atcgaatggc ttgctaacgc
     2641 acacaggttg gcaatcgact gctcaacatc atgcgccacg atcccttcat tgccggtcac
     2701 ggcggtatca tccagcgcca ttaacaccgc tttccacgca gccgaagcac tggtcgaaac
     2761 cttcatcgcg cagctgttcg acgcaccatc gcaaatcatg ccgctgacat cgccgatcat
     2821 actgctgatc gccatcgaga tggtttcata acgcccatcc accagccatg ccatcccggc
     2881 ggcggccccc attgctgcgg tcgttgcggc acaaagtgca gacaaacgcg gtaactggtt
     2941 atggatgtaa attgcgctca aatgagaaag catcagcgca cgcgccagcc gttcatcatc
     3001 cgctccgaag tgttctgcta ccaccaccac aggcattgtt gcggtgatcc cctggttacc
     3061 cgagccggag ttactcatag ccggaagcgt agcgccgccc atacgcgcat cggatgccgc
     3121 gctggtacga atcacaatgg atgaagagag atctttcgcc agcaaaccgc gctcgcactg
     3181 tttttccagc gtcgcgccaa tatgcagccc ccacttaccg ctcaaacctt cctgcgataa
     3241 cgcacaattt agcttcgcgg aatcgagaat aaagcggatc gccgcaaacg ggacttcatt
     3301 gacgaacttc aggatctcag ccagcgtcgt tctggaaagc accgtcagcg gagactcttg
     3361 ctcgccctct gccacacacg cctgctgggt aaacaccaca ccattgtgcg tctcaatatg
     3421 cacaatgttg gtatgcccgc cgacgatggt gacacacgcc cacttctcac cgttccagac
     3481 tttggcgcgt gagaagagga tttcattgca aggttcctgg atcttaacgg agactttccc
     3541 cgccgccagc agtgctttgg catcggcaat tgcctgcgca gttgcgtctt tcagcacttc
     3601 cagcccagct ttggcattcc cacctaatgc tcccagcgcc gccgcaatcg gcagccccac
     3661 cattcccgtg ccgggaacgg tgacgcccag accgttcttc atcagatttg gcgaaaccca
     3721 ggcttctaca cgttcaaccg gaccttccag ttctgccgca gcaaccgccg ccgccagcgc
     3781 cagtgaaatc ggttcagtac atcccagcgc cggttttact tcctcctgaa cggcgaggat
     3841 gtaacgctgc cataacggat ttaaagtcga atcaaacata ttaaaaacct taaaatttca
     3901 ggtaaatcaa gaaaatgcca ggaacggaga aacacaaagc aacaaaccag tgacgataat
     3961 caggtacaga gacatccctt tgtatttgtg caatgccggt actttgtaaa ccagccacgc
     4021 cgggatcagg caccctacca tgccgaaaat cgggctacag atagaggtga agctcaacac
     4081 cggtgcgttc agtacgatgg cgctccaggc cagcaaaatg gcgaaaatca tgatgccgcg
     4141 ctgaacaaga ttttcgttaa tcttctcggc aggcatcttg cgacgcagga tgttcattac
     4201 gatcccttgc gttgcttcgc gaaagcctaa atagacgcca aagaatgcag tcatgacagc
     4261 aaagatattg agaatgacgc tgaccacttt cacccacgct gcgccgtcac cgctaataaa
     4321 ctgtgcggca atcgccagcg cggaaatatt ctgctcatag gcttttaccg cttcgtcatg
     4381 ccccatcgcc agcgtgaacg acacggcgta gaaaaagacg gtgacaaaca aaatgccaaa
     4441 cgcgatattc attgcccgca atgctttatg ccgcgccact tcaatagatt tttcccgcga
     4501 gcgataagag atcaccatcg ggcttaacgt ctggataaac aaaatcgacg tcagggtaaa
     4561 cggcagcgta ataatggcgt ttttcaccag cagccccagc ggcggtagcg aaccgacgtt
     4621 atacagatgc cacattccca ccatcgacac gcccagcgcc gcgaccacca gcagcttggt
     4681 cagcaccatg ccggtcgaaa ttttgaataa caatttctcg ccgcgtgaag agatcgccac
     4741 cagaatgcaa atcagtacca gaccataaaa gggactgtct gacagcaacc cttccgtcac
     4801 gccgaaggta tgcagatagg aagcactatc gttggtgatg gcggtggaat agacgaacat
     4861 ccaaatcacc agcattacga aatagagcgc acctaacagg atgccccagt ttttacctaa
     4921 ataaccgcta atgacactcg ggtaatcttt acattctggt gattctgcca gcgtattaat
     4981 aaacaaccgc tgaaacagat acattgccgg gtaaccaatc accgatgaga gcaaaaatac
     5041 ccacaatccc atcaaaccga cctgcaccgg gagaaaaaca atccccgcgc caatcgccat
     5101 cccgatactc ataatcaccc agccggtgtc agtactgtcg aatttgatcg cttctcgcca
     5161 ctcgctctca ctcattccag cacgacccgc cggggtcgaa gcgtctgcaa tgacgccttt
     5221 attcgatgca atttccataa tttctcgctc aatattttgt agggcttatt atttttttcc
     5281 gagccgcatc aaggcgatat gcggtctacg tttttgcagg cgagttatta gaataaagaa
     5341 atgatacgcg cgaggtcaga gaaaatcttc acaatcaaga ctttgaaata acggagatgg
     5401 ataagaattt tctactaaat taatcgcaag agaatattca ttctctattt gcggcagatc
     5461 acaaaaaaaa ggtgcacatt tgtgcaccca aggatgaaag ctgacagcaa tgtcagccgc
     5521 agaccacttt aatggccagt cctccgcgtg atgtttcgcg gtatttatcg ttcatatctt
     5581 tgccggtttc atacatcgtc tcgatcactt tatcgagtga aacacgcggt gccgaggtgc
     5641 ggcgcatcgc catccgcgcg gcgtttactg ctttcacggc attaatggca ttacgttcaa
     5701 tgcacgggat ttgtacctgt ccggcaaccg gatcgcaggt cagcccaagg ttatgctcca
     5761 tcgcgatttc cgccgcattg catacctgcg ccggactgcc gcccagtagt tcagttaacc
     5821 ctgccgccgc cattgaacag gccacgccaa tctccccctg acagccgact tccgcgccag
     5881 agatggaggc gttcatttta tacagcgcgc caatagcccc cgcggccaga aaatagcggg
     5941 caattgaccg ctcgtttacc ggacgacgga acttatcgta ataagccagt acagccggaa
     6001 taatgccgca cgcaccgtta gtcggtgccg ttaccacgcg cccgccagct gcgttttctt
     6061 cactaaccgc cagcgcgtac atgttgatcc agtcgatgac attcatcgga tcgttagaga
     6121 tgttatcgct ggaaaccagc tgacgacgca gcgctacggc acggcgcggc acattgagcg
     6181 gaccaggcag cacgccttca gtgttcatcc cacgttcaat accgtcatgc atcacttgcc
     6241 agatacgggc aaaaccggcg tcaatttccg ctttgctgcg cagcgctagc tcgttgtgca
     6301 tcatcagacc agatatagac aggccgttgt aatcacacat tttcagcagt tcacctgctg
     6361 agtggaaatc gtaaggtacg gacgtttcga catcgtgcga caggccgaag tgttcttctt
     6421 cgacaattaa cccgccgccg acagagtaat aggttttact taatagcgct tcctgtccct
     6481 tccaggcagt gatccgcatt ccgttctcat ggcgaggcaa catttcggga tggaagataa
     6541 tgttctttgc tacaggaaaa tcaacaatat gcgcaccaga tgccactggc agccgcccgc
     6601 tgcgcgttac taactctata aatgcaggga tctcatcaat gacaacatcc tgcggactgt
     6661 ttcctgccag tcccatgatg atggcgacat ccgtggcatg gcctttgccc gtcagtgaca
     6721 acgacccgta cagatcgacc acaatatggc tcgtcgcggt taataagccg ctactttcca
     6781 gccgatcaat aaaacttttt ccggcattca ttggccccac ggtatgcgaa ctggagggac
     6841 caatcccaat tttgaaaata tcgaatgcac taatcatatc cacaccctcg gattgccgtt
     6901 cagtgaagtg gagcggaacg accttacgac cgtcccgctc acgaggcttt acgcactacg
     6961 tactgcgatg gcttcaattt ccagcttcac atctttcggc aaacgcgcga cctgcacaca
     7021 gctccgggtc ggataggtcg cctgatgctc atcgaagaac tgcttataga cttcgttgat
     7081 ggtggcaaaa tcattcagat cggtgataaa cacggtcatc ttgatgatat cgcccacgct
     7141 cagcccggca gcaaccacga tcgctttgac gttttcgagg cttaaacgcg cctgatcttg
     7201 cacatcagcc gggatctcac cggtctgtgg gcaaaccggt atttgcccgg aggtgaagac
     7261 catgctgcct aaatcaacgc cctgaacata agggccgatt gcgcctgggg cacgttgcgt
     7321 ttcgataatc tttttcatac atcctccggc gtcagagcgc ctgggtaaag gtacgtgaaa
     7381 taacatcctg ttgctgttca cgggtcagtg cgttgaagcg cacggcgtag ccagagacac
     7441 ggattgtcag gttaggatat ttttccgggt gctcgatggc atccagcagc atttcccgat
     7501 tcattacgtt gacgttgagg tgttgaccgc cttcgacatc cgcttcgtgg tggaaatacc
     7561 catccagcag gccgacaagg ttggttttac gtactggatc ttctttgccc agcgccgcag
     7621 gcacaatcga gaaggtgtac gagatcccgt ctttagcgta ggtgaacggc agtttcgcca
     7681 ccgacgtcaa tgaggccacg gcacctttgc ggtcacgacc atgcatcggg ttagcgcccg
     7741 gcgcgaacgg tgttccggcg cgacgaccgt ccggcgtgtt accggttttc tggccgtaca
     7801 ccacgttaga agtgatagtc agaatcgact gggtagggac ggcgttgcga taggttggca
     7861 gcgctttaat tttcttcata aaacgttcaa ccaggtcgca ggcaatgctg tctacgcgct
     7921 cgtcgttgtt gccgtactgc ggatattcac cgtcgatttc aaagtccacc gccaggccgt
     7981 tttcgtcacg gattggtttc acgcgggcat atttgatggc agacagggag tccgtcgcca
     8041 ccgacaggcc cgcgatgccg cacgccatgg tgcgatagac atcacgatcg tgcagcgcca
     8101 tcagcgaagc ttcgtagctg tacttgtcgt gcatgtagtg aatgatattc agcgcgctga
     8161 tgtactgcac cgccagccag tccatgaagt gatcgaggct gtccatcact ttgtcgtaat
     8221 ccagcacgtc gtccatcaac ggtgctgttt tcggcccgac ctgaatcttc agcttctcgt
     8281 ccaccccgcc gttaattgcg tacagcagtg ttttcgccag gttagcgcgt gcaccaaaga
     8341 actgcatttg cttaccaatc accattgggc tgacgcaaca agcaattgcg taatcgtcgc
     8401 tgttgaagtc agtacgcatc agatcgtcgt tttcatactg caaggaagag gtgacgatcg
     8461 acacctgcgc ggcatatttt ttgaaggcaa tcggtaattc ttccgaccaa agaatggtca
     8521 ggttaggttc cggtgccggc cccatagtgt gcagggtgtg caaatagcgg aaggagtttt
     8581 tggtcaccag cgtacgaccg tccagcccca tcccgccgat cacttccgtc gcccagattg
     8641 ggtcgccgga gaacagcgaa tcaaattccg gcgtacgcaa gaagcgcacc atacggatct
     8701 tcataatgaa gtgatcgatc agttcctgtg cctgctgctc attgagtacg cctgctttaa
     8761 agtcgcgttc aatgtagata tcgaggaacg atgccgtgcg ccccagcgac atcgcgccgc
     8821 cgttctgtga tttcactgcc gccagataag cgaagtagag ccactgcacc gcttcctgcg
     8881 cattctgcgc cgggcgagag atatcaaagc catatttcgc cgccatttcc tgaatctgca
     8941 acagcgcatg acgatgctct gccagctcct cacgcagacg gatggtggct tccagatcct
     9001 cgcctttttc cagacgagac tggagatcgg caaattgcag ttcgcgttca cgcaccagat
     9061 aacgaatgcc atacagcgct acgcggcgat agtcaccgat aatgcgccca cgtccataac
     9121 catctggtaa accggtcagt acgccagatt tacggcagcg cagcatatcc ggtgagtaaa
     9181 catcaaatac gccctggtta tgggttttac gcagatcggt aaacagatat tcaaattcac
     9241 tgtccatttc tcggccatag gcgtggaatg aacttttaat catattaatg ccaccgaacg
     9301 ggtgtagcgc acgtttcaac ggcgcatccg tttgcaggcc aacaattttt tccagcggct
     9361 ggttaatata tcccgcatca tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa
     9421 ccggcgcgtg ggttgcgttt tcgatacgga tgccttccat tactttttcc cacaattccg
     9481 tggtggcagg cgtcgcttcg gcgaggaaag attcatcgcc ttcatacggt gtatagttat
     9541 gttgaataaa atcgcggaca ttaatttcgt ttttccagtc cgtaccttta aagccaagcc
     9601 atgcgtcggc gtacagctta tcgctggtat caatatctac cttcatgaaa aataatctct
     9661 ctacaatact tcaactaaat tatgcaaatt ctgcgggcgc gttaactttg cctaaatgaa
     9721 tggcatccaa agcaatcatt ttttcttcgt tagtcggaat aacggcgcaa atgacatgcg
     9781 cattttcact ggaaacaatt cgctcgccaa aagagttaga tcgattattc atttctgtat
     9841 caatctctac gcctaataca gccaaatgtt ccatgaccag gcgacgtaat gaagctgcgt
     9901 gtccggcaat atgacgggca attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt
     9961 cgtgaccttc atgccaggct ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac
    10021 ctaataatcc cgactcttta ttcactacgc gttcaaggtc acccaggctc tggttggttt
    10081 ggctggcgac ccaggacatc gcaccaaagt cgacatcgcc actgcgggta cccatcatca
    10141 agccttccag cggcgtcatt cccattgagg tatcaacact ctgaccgttg cgaaccgcgc
    10201 agattgacgc gccattgcca agatgcgcca caaccaggcc ggagtcatct tccgccagat
    10261 tcagcagcga atgtgcgcgc tgggaaacat agcggtgcga cgtgccgtgg aaaccataac
    10321 ggcgtacacc taactcttca taatatttcc acggcaggcc gtataaataa gcttccggag
    10381 ccatcgtctg gtggaaactg gtatcaaata ccgccacctg agttacgccc ggaaataatt
    10441 gctgcgccga ttcaatacca cttaaattgg cgtaattatg caggggtgcc agtggagaaa
    10501 cgcgacggat attatcaatg acttcatcgg taataatggc ggactcggta aaaatactgc
    10561 cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc atttaaattc cgtttttcca
    10621 gttcaaatgc aattgccttc aatgcacctt cgtagctgtg gtgagccagc ggtgctggct
    10681 ctcccccatt tacggataag aatgcatttt ccgagttaat accgtcggca atacctgaca
    10741 ttaatacttc acagtcgctg gcatcgagca cggaaaactt aatcgaagac gaaccacagt
    10801 taataaccaa aacaaccgga aattcattca tctcttttct catcctgagt tacggattaa
    10861 aacagtttgt atacgatgtt caggatggtc agcagaccaa tcacggtaac aaacacgtta
    10921 tccagacgac cacggtattt cgccagagac ggcgctttac ggatggcata catcggcaac
    10981 aggcacagca gggatgcgat aatcggtgcg cccatggctt caatcaggtc aaggatgttc
    11041 gggttggcgt aggcaacaac ccaggtggag cccatgatga agatcatgct gatagtgttc
    11101 agtttaccca gcgacacttt agttttgtcg cctttataac caaacttcag gaccaggcca
    11161 ttcagacctt ccagcgttcc cagatagtga ccgaagaaag atttgaagat agccacgagt
    11221 gcgatgatgg aagccgcata ttccagtgta atcgcgaacg ttgttttggt accggtcatg
    11281 gacgcaaagt ggttagccag ataagaaagc actggaatat tctgcgcttt ggcttccgcc
    11341 atgttggccg gagacagcgt aaacaggcag ctaaaggcaa agaacatcac cactgcaacc
    11401 atcagcatgc tggcacgaga aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg
    11461 aagtctttct catactcttc acgtttagaa accacgaagg aagagacgat tggcgagaaa
    11521 ttaaaggaga aaaccatgat ggaaatcccc agccagacag tgatcaggat accgtcatga
    11581 ccggttaacg acagcgaacc gaggtcaacc tggtcgataa ccgcagagtt ccagtaaggg
    11641 atcagcgaca gagaaatcag caccaggctg gcgataaacg gccataccag gtagctcatc
    11701 actttaacca tcagatcctt accaaaccag atgacgaaag ccatcagcag caacaggaac
    11761 agcgccacaa agccgcgatt cagcggtgca aatccgagct ggttttccca gaacgtcata
    11821 aaggtattgg taatagtaac gccataaatc cacagcagtg ggcaaatcgc gaagaagtac
    11881 aggaacgtga taaccacgcc gccagtttta ccaaaatgct cttccaccgt ttccgtaatg
    11941 ttgccggaag ggttagagcc agaaagacac agacgcgcca gcgcccggtg gcaataaaat
    12001 gcgatggggt atgccaatac caacatcaga agaatcggga tcagtccgcc aaaacctgcg
    12061 cggataggga agaacagcac cccggcgccg attgccgtac caaacaagcc taacgtccat
    12121 gtggtatctg atttacgcca ggacgattgt tttgtctggc tggatacaat gctatctgaa
    12181 gtactcatat cctatcctca acgaattaat taagcgtcaa cgaaaccggt gatttgagag
    12241 acgcgagaaa gatcgatatt gccgccggaa ataatactga cggtttttct gttttgaata
    12301 tattggtcta atttaccgct taataatgca gcacatgcca gagcgcctgc gccttcggtg
    12361 acgactttat tgcgctgaat taaggcaatc atactgtttc tgatttcgtc ttcgctgacc
    12421 agcacgatgt catcgactaa ttcacgaacg atttcgtaag ttaaattacc cgggcgggag
    12481 acatcacaac catccgccag ggtgccggta gttcggtgcg tggttatttc tccggagtgg
    12541 aaagaagccg ccatgccgtg aacgttttct gactgtacgc caataacacg aatggtcggg
    12601 ttaatagatt taattgccac cgcaatacca gcaattaaac caccaccacc aattggcaca
    12661 atcacgttat cgacatcata gagatcttcc ataatttcca gaccaatcgt tccctggcca
    12721 gcaatcactt tcggatcatc gtaaggtggg ataaaaatac gaccttccat ttcgacaatt
    12781 tcgctcactt tagcgatagt gtcgttgaaa ttatcaccat gcagaacgac ttctgcggag
    12841 tagtcgcacg ttgccgctac tttggatttt ggcgcacctt ttggcatcac cactttaccg
    12901 tcgataccca gcatcgcgca ggagagcgaa accccttgcg catggttgcc cgcagaacag
    12961 gccactacac ctttacgttt ttccgcatca gtcagtgaac ttaatttatt aaatgcgcca
    13021 cgaattttaa atgaacccgt acgctgcata ttttcaaact tcaggaatat ttcacctttg
    13081 caacgttcac taaaatagtt tgagcgaggc atgcctgttt tataaattcg cccagccagt
    13141 cgttgtttcg cttcaataat gtcatcaata gcaaccggca gatcgtatgt aatatgcatt
    13201 ataaaacctc ttcgcctgaa ttaaatatgt aggtaaccgt aaccgacacc tgcaaaacag
    13261 acaggtggat tatttatgtt taaataaaaa caaataacta accaacttct attaattgcc
    13321 ttcgtctaca cccattataa gatgaatact ctttggctaa ttccaccaaa accgatgctg
    13381 cttttttaat acgataattt ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt
    13441 cttcaaccgg aatagtaata aattgatttg aaccaaaagg tgacgtcata tcacaaggaa
    13501 ttacagttaa gaaatcagca ttgagaacaa gattataaat tgtcacgact gagtcggttt
    13561 taacgatgtt ttcaatactg atgccatttc tttgtaacgt agtaagaagc tcgctgtagt
    13621 accccatatt ggtttgtggc aacacccact gttcgttctt caacgactcc agcgtggtgg
    13681 tgccggtgca tgttcgggac ttactggcta ccagcacaaa ctcggactcg aacagcggct
    13741 caacatgtaa atcctgaagc ttcatttctg cacttaacgt accaatcgca aaatccagtc
    13801 gaccgtcgcg gattgccggt aagaacgaag acagttgcgc ttcatacata gaaacctgcg
    13861 ctttcgggaa cacctctttg aacttgttga tcatccctga cataaaagta aaaccaatca
    13921 atgaaggaaa accaaatgag acttccacca ccgcctcaga agacataccg cttatctcat
    13981 taaccatatt tttcatttca cgggtaatgg attcggaacg ggagagtaac aattgaccgg
    14041 caggtgttaa tgttacaccg gtatttttcc gcaccactaa ttccacacca aaataatctt
    14101 caatatcgtt aatgatttta ctgacggctg gttgagttaa ccctaattct tttgcagccg
    14161 agccgataga gccacttcta atgacttcct gaaagactac caggtgctgc gttttcggaa
    14221 gaagaatagt gctcataacg acctacgtta attacctcat tgacggcatg aa
//
LOCUS       DAZFAH010000111        14269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFAH010000111 DAZFAH010000000
VERSION     DAZFAH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.111"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1224
                     /gene="citC"
                     /locus_tag="ACHJWB_003115"
     CDS             166..1224
                     /gene="citC"
                     /locus_tag="ACHJWB_003115"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4314699.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAARCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLCYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1239..1535
                     /gene="citD"
                     /locus_tag="ACHJWB_003116"
     CDS             1239..1535
                     /gene="citD"
                     /locus_tag="ACHJWB_003116"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4314700.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEVLLARASGIPALPWEDCQ"
     gene            1532..2440
                     /gene="citE"
                     /locus_tag="ACHJWB_003117"
     CDS             1532..2440
                     /gene="citE"
                     /locus_tag="ACHJWB_003117"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4314701.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2451..3983
                     /gene="citF"
                     /locus_tag="ACHJWB_003118"
     CDS             2451..3983
                     /gene="citF"
                     /locus_tag="ACHJWB_003118"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4314702.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3987..4538
                     /gene="citX"
                     /locus_tag="ACHJWB_003119"
     CDS             3987..4538
                     /gene="citX"
                     /locus_tag="ACHJWB_003119"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4314703.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="ACHJWB_003120"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="ACHJWB_003120"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4314704.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     LRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5442..6905
                     /gene="citT"
                     /locus_tag="ACHJWB_003121"
     CDS             5442..6905
                     /gene="citT"
                     /locus_tag="ACHJWB_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4314705.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7019..7825
                     /gene="rna"
                     /locus_tag="ACHJWB_003122"
     CDS             7019..7825
                     /gene="rna"
                     /locus_tag="ACHJWB_003122"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4314706.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8055..8465
                     /gene="rnk"
                     /locus_tag="ACHJWB_003123"
     CDS             8055..8465
                     /gene="rnk"
                     /locus_tag="ACHJWB_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4314707.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8603..8692
                     /gene="yldA"
                     /locus_tag="ACHJWB_003124"
     CDS             8603..8692
                     /gene="yldA"
                     /locus_tag="ACHJWB_003124"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4314708.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8696..9934)
                     /gene="ybdR"
                     /locus_tag="ACHJWB_003125"
     CDS             complement(8696..9934)
                     /gene="ybdR"
                     /locus_tag="ACHJWB_003125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4314709.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQI
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVIVVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10155..10583
                     /gene="uspG"
                     /locus_tag="ACHJWB_003126"
     CDS             10155..10583
                     /gene="uspG"
                     /locus_tag="ACHJWB_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4314710.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10704..12269)
                     /gene="ahpF"
                     /locus_tag="ACHJWB_003127"
     CDS             complement(10704..12269)
                     /gene="ahpF"
                     /locus_tag="ACHJWB_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4314711.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DKYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12440..13003)
                     /gene="ahpC"
                     /locus_tag="ACHJWB_003128"
     CDS             complement(12440..13003)
                     /gene="ahpC"
                     /locus_tag="ACHJWB_003128"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4314712.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13375..14121
                     /gene="dsbG"
                     /locus_tag="ACHJWB_003129"
     CDS             13375..14121
                     /gene="dsbG"
                     /locus_tag="ACHJWB_003129"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4314713.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWIDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3400 a         3762 c         3579 g         3528 t
ORIGIN      
        1 atttcacgct gcaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
       61 cacccccctt tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt
      121 cgttgcaaat cgataacaac atgtaccttc aggatactat ttattatgtt cggcaatgat
      181 attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      241 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      301 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      361 gaatccgttc gcggtgaagg actggcgctg acattagcca ctgaattgat aaacctcgcc
      421 tatgagcggc acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc
      481 cgccagtgcg gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac
      541 agcgccacgc gactgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac
      601 aagattggct gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt
      661 caacaggctg cggcacggtg tgactggttg catctgtttt tagtcaaaga agattcttca
      721 cgcttcccct atgaagaccg gctggatctg gtgttaaaag gcaccgccga tattccacgc
      781 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      841 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt
      901 cagtacctcg ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt
      961 cgcgttaccg cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc
     1021 gcaccgccca tcgaactggt tgaaattgag cggctgtgtt accaggagat gccgatatcc
     1081 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1141 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1201 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1261 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1321 atatcgacct gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1381 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1441 aaggcgcact ggactgcatt ttacgtgcac gactggaagt cctgctggca cgcgccagcg
     1501 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1561 aactcgcacc cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa
     1621 ctccttcatc tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg
     1681 tgaaaaagac accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga
     1741 tattgaaacc attgtgcgtg taaacgcgct ggattccgaa tggggtgtca acgacctgga
     1801 agccgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1861 tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1921 cagcaccggc ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat
     1981 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2041 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt
     2101 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2161 cgaagccgga ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc
     2221 gctgatcaac ccgcgtcaga ttgatctgtt gcacaacctc tacgcaccaa cccagaaaga
     2281 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2341 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2401 gctctctcgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2461 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2521 atcttgccgc tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2581 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga
     2641 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2701 tcatcgcgaa gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc
     2761 atgcgccgtt ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc
     2821 tgcgcggtcc actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcagatcc
     2881 actctcacgg cggtcgtgtg catctggtac agagcggcga actgaatatc gacgtggctt
     2941 tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct
     3001 gctgcggctc cctcggctat gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc
     3061 ttaccgaaga actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg
     3121 atttaatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc
     3181 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca
     3241 actcaggcta cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg
     3301 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3361 ttggcggtat taccgcgacg atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc
     3421 tggatgtgca gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca
     3481 tcgaaatcag cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc
     3541 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3601 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct
     3661 ctgcgctttc catcatcgtc gcgccgcttg tacgcggtcg tattccgact ctggtggata
     3721 acgtgctgac ctgcatcacc ccaggctcca gtgtcgatat tctggtcaca gaccacggta
     3781 tcgcagttaa cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag
     3841 tggtttccat tgagtggctg cgcgaacgcg cgcgtctgct gaccggtgaa ccacagccga
     3901 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     3961 ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4021 cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct
     4081 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4141 aagacagcga ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag
     4201 ccgcaaaaca gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4261 agggcatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4321 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa
     4381 ttctctcccg ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg
     4441 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4741 gcctgtagtg cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt acgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4861 ttttctttag ggctgctatg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggta
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggtctgac cgatcgcgaa
     4981 ctgcgtacca ataattcaca actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcgggt tatccactag tgctcaacca cgccctgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5161 ctcctactga tggcgatcaa cggcgatacc aacgttgcgt cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctact gatccttacc tggtttttag cacagattta attatttaag
     5401 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc
     5521 ccgacggtat gccgccgcag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5821 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attccccaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6121 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6181 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6241 aagaagtggc aacctgggcg ggtgatgaac tgaaaaccat gggtgcgctg acacgcagag
     6301 agtggacact gattggcctt gtattgctca gcttaggttt gtgggttttt ggcagtgaag
     6361 tcattaatgc tactgcagtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg
     6421 tgccttggaa agatattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6481 ctctggttgt gatggctaac ggcctgactc gttctggctt tattgactgg ttcgccggta
     6541 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6601 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6661 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6721 tgctggtgct gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6781 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6841 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6901 actaatacgt tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc
     6961 gggctttttt atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat
     7021 gaaagcattc tggcgtaatg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7081 caacgccgta gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7141 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7201 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7261 gccaggattg cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt
     7321 cggttgcgct actcgcccaa tcccgaatct accagaagcg cacgccagcc ggatgtgttc
     7381 atcgccggaa accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg
     7441 agctggcgga cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg
     7501 ttttgatccg gacgcatact tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag
     7561 cgaagccgga aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga
     7621 cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca
     7681 gggtaaccct gcgtatttga ctgaaattca gatctcgatc aaagctgacg ccatcaacgc
     7741 tccgctttct gcaaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt
     7801 tgtgattgat aaagtgggtt attaatcatc tgatttacaa aacacgctaa ttcgtcttcc
     7861 ataaaccgtg acgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt
     7921 gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg
     7981 ccgcctgacg gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac
     8041 gacatggagt aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca
     8101 tcgatattct gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg
     8161 cagagttgga tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa
     8221 tgaacagccg ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg
     8281 tgtatccggc aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg
     8341 ccgcactgct ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg
     8401 caacccacct tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc
     8461 tttaatccca tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct
     8521 ttacgcaaaa tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc
     8581 cgtttcgttt gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat
     8641 tatggcggcg attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgatcata
     8701 ttgttccccc cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc
     8761 tttgtgcacc gggtaccaca atcaccttac ggcactcctc ttcacgtttt tcgaaaatct
     8821 catatccccg ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca
     8881 gtaatccttt ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tggatctgtc
     8941 ccattttaaa cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc
     9001 cagcgtagac gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc
     9061 gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc
     9121 tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg
     9181 tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc
     9241 cgtagcggtc agcggcgaaa ctcaagcggt agggatgatg atcaacaaca aaaatctgtt
     9301 ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga
     9361 ctgcaacgct tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca
     9421 gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc
     9481 ccacattccc tttagggacg cggacatatt ccgcctgtcc accaggaacg ccaccataca
     9541 ggtgactata accaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc
     9601 ctttacccgc attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt
     9661 cgccacaagc aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt
     9721 cctttccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa
     9781 cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa
     9841 tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa
     9901 cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa
     9961 gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggtttttga
    10021 agaatttctg gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga
    10081 accagacaaa tatcagggat aaatattata atcatggcga atgatttgtt catgattaac
    10141 agggagaaag ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat
    10201 tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc
    10261 tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc
    10321 gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc
    10381 acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg
    10441 aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc
    10501 catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc
    10561 tgccggtgct ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc
    10621 ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa
    10681 ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca
    10741 ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc
    10801 gtacagtcac ccgctgcgaa tacgcctttg acgttggttt cgcatttcgc atcaatgata
    10861 atctcaccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga
    10921 ccaatctgga cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct
    10981 cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc
    11041 agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc
    11101 atttctggcg caaattccag cagcgttacg tgctcaacga tacccgccag gtcaattgcc
    11161 gcttccacgc cggagttacc gccgccgata accgctacgc gtttaccttt aaacagcggg
    11221 ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat acttatcttc gcccggaacg
    11281 ttcatgttgc gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg
    11341 ccagaagctg tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg
    11401 gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct
    11461 gccagcttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg
    11521 aggatctgac caccaaaacg ttcgcccatc agacctgtac ggatgccttt acgtgcggag
    11581 taaattgctg ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca
    11641 cgcttgttca gctcttctgc cgcacgtttt tccgcgcccg tatcaatttt ggcaacgatt
    11701 tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg
    11761 cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc
    11821 ttgatgcgcg ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagttg
    11881 tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc
    11941 tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc
    12001 agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct
    12061 gggttggtga tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg
    12121 actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg
    12181 ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg
    12241 agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg
    12301 caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg
    12361 gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgccg gagttggtag
    12421 cgcgaaagac gaaggaaatt tagattttac caaccaggtc cagagacgga gccagagttg
    12481 cttcaccttc tttccattta gccgggcaaa cttcacctgg gtgagaagct acgtactgtg
    12541 ctgctttgat tttacgcagc aggtcagacg cgtcacggcc aatgccttca gcggtaactt
    12601 cgattgcctg gatgataccc tgcgggtcaa caacgaaggt cgcacggtca gccagacctt
    12661 catcttcacg catgttgtcg aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg
    12721 catatttgat tttagcgatg gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag
    12781 tatcggtaga tactgcgtat acgtctacgc ccagtttctg cagttcttcg tagtggtcag
    12841 caacgtcacc cagttcggtc gggcatacga aagtaaagtc agccgggtag aagaagaaga
    12901 cgctccagcg gccttcggta tctttttcgg tgatttcgat gaattcgccg tttttgaatg
    12961 cctggttttt aaaaggttta attttggtgt taatcaagga catctatact tcctccgtgt
    13021 tttcgatgag atgtaaggta accaattttt gccgattcgg ctaatgcgtt tccattatca
    13081 aatcgataag ttttacctta caaccttcgt aagacaactt tgtgaacttt gcaccgccag
    13141 cggcgataaa aagtaaaatg gccccctttc caggtgaccg tgtaatctga actacccttt
    13201 ggtatcttca gcgcctgcct gcaggctgag gtgttgccct tacaaatgca acaacgacat
    13261 ggattacaac accctcataa acaaagggca atcacctgat ctaagctctt acctatgaca
    13321 gtgataggtt atgcctttta ctcgactttt gcactgactg aaaaggacaa attaatgtta
    13381 aaaaagatac ttttactggc tctgcttcct gcaatcgcct tcgcagagga acttcctgct
    13441 ccagtaaaag cgattgaaaa acagggcatt acaatcatca aaacgttcga tgcccccgga
    13501 ggaatgaaag gttatctcgg aaagtatcag gatatgggcg tcaccatcta cctgactcca
    13561 gatggtaagc acgctatctc tggttacatg tacaacgaga aaggtgaaaa cctgagtaac
    13621 acacttatcg aaaaagaaat ttacgcacca gccggacgcg aaatgtggca acggatggaa
    13681 caatcccact ggctcctcga cggtaaaaaa gatgcgccgg tcattgtcta cgtgttcgcc
    13741 gatccgttct gcccatattg taaacagttc tggcagcagg cgcgcccgtg gatagattct
    13801 ggcaaagtgc aattaagaac attgttggtt ggggttatca agccagaaag cccggcgaca
    13861 gcagcggcaa ttcttgcctc caaagatccc gcaaaaacct ggcaagaata tgaatcctct
    13921 ggtggcaagc ttaagctaaa cgtgcctgca aacgtaagta cagaacaaat gaaagtgtta
    13981 agcgccaacg agaaactcat ggacgatctg ggggcaaatg tcacaccagc tatctattac
    14041 atgagtaagg aaaatacgct gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat
    14101 atcattatgg ggaataaata aaagataatt catggatggt tgaaatacat ccctgctttt
    14161 caaccatcct aaaaaagcat gaattgctcg ctctgcccta agtggctatg ctattttaga
    14221 aaaacttaat cctgagttaa gcaaaaaaat ataatcaata aaaatatat
//
LOCUS       DAZFAH010000112        14115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFAH010000112 DAZFAH010000000
VERSION     DAZFAH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.112"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..2609)
                     /gene="copA"
                     /locus_tag="ACHJWB_003130"
     CDS             complement(105..2609)
                     /gene="copA"
                     /locus_tag="ACHJWB_003130"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4314714.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            2871..3803
                     /gene="glsA"
                     /locus_tag="ACHJWB_003131"
     CDS             2871..3803
                     /gene="glsA"
                     /locus_tag="ACHJWB_003131"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4314715.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            3806..5098
                     /gene="ybaT"
                     /locus_tag="ACHJWB_003132"
     CDS             3806..5098
                     /gene="ybaT"
                     /locus_tag="ACHJWB_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4314716.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            5129..5226
                     /locus_tag="ACHJWB_003133"
                     /pseudo
     CDS             5129..5226
                     /locus_tag="ACHJWB_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5223..5630
                     /gene="cueR"
                     /locus_tag="ACHJWB_003134"
     CDS             5223..5630
                     /gene="cueR"
                     /locus_tag="ACHJWB_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4314717.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(5631..6089)
                     /gene="ybbJ"
                     /locus_tag="ACHJWB_003135"
     CDS             complement(5631..6089)
                     /gene="ybbJ"
                     /locus_tag="ACHJWB_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4314718.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(6086..7003)
                     /gene="qmcA"
                     /locus_tag="ACHJWB_003136"
     CDS             complement(6086..7003)
                     /gene="qmcA"
                     /locus_tag="ACHJWB_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4314719.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            7149..7826
                     /gene="fetA"
                     /locus_tag="ACHJWB_003137"
     CDS             7149..7826
                     /gene="fetA"
                     /locus_tag="ACHJWB_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4314720.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7813..8592
                     /gene="fetB"
                     /locus_tag="ACHJWB_003138"
     CDS             7813..8592
                     /gene="fetB"
                     /locus_tag="ACHJWB_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4314721.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(8655..9509)
                     /gene="cnoX"
                     /locus_tag="ACHJWB_003139"
     CDS             complement(8655..9509)
                     /gene="cnoX"
                     /locus_tag="ACHJWB_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4314722.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(9570..10379)
                     /gene="ybbO"
                     /locus_tag="ACHJWB_003140"
     CDS             complement(9570..10379)
                     /gene="ybbO"
                     /locus_tag="ACHJWB_003140"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4314723.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVSALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(10369..10992)
                     /gene="tesA"
                     /locus_tag="ACHJWB_003141"
     CDS             complement(10369..10992)
                     /gene="tesA"
                     /locus_tag="ACHJWB_003141"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4314724.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            10963..11649
                     /gene="ybbA"
                     /locus_tag="ACHJWB_003142"
     CDS             10963..11649
                     /gene="ybbA"
                     /locus_tag="ACHJWB_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4314725.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            11646..14060
                     /gene="ybbP"
                     /locus_tag="ACHJWB_003143"
     CDS             11646..14060
                     /gene="ybbP"
                     /locus_tag="ACHJWB_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4314726.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAITGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         3084 a         3798 c         3897 g         3336 t
ORIGIN      
        1 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
       61 tgccaaatgt gccaccctaa agcagcgcat ccgcaatgac atacttattc cttcggttta
      121 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
      181 ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc
      241 ggaataccga tactgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
      301 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
      361 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
      421 tgggccagcg ctggcgcatc gttaatgccg tcgcccacca ttgccacctg acgtccttca
      481 ctttgcaggc gtttaatcgc ttcggcttta ccgtccggca gcaccccggc gatcacttca
      541 tcaatcccgg cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
      601 accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaat
      661 ggatcgcgta ctgccagcag ggctaccact ttcccgtcaa ccgccagcag cacaggcgtt
      721 gccccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
      781 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
      841 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
      901 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
      961 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     1021 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     1081 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     1141 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     1201 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     1261 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     1321 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     1381 gtatggctgc caaccgcact ggcacgaaac agcacgctac cgtcctgtac cactgtcccg
     1441 gcatggacgc tttcgccttc gcctttttgc tgcgggattg gttcgcccgt cagcatcgct
     1501 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     1561 gtcaggcgca gcaacatacc tggctgcact tccgccagag gcacgctttt ttcaccttcg
     1621 tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     1681 gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc gataatcatc
     1741 gcgctggctt cgtaataaag atgtcgcgct tccatcggga accactgcgg ccacaggttg
     1801 acgctcatcg aatagagcca cgccacgcca gtacccagcg ccaccagcgt atccatcgtc
     1861 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     1921 atcaccgcta gggttatcag gccgataacc aaccacaggc tgcggttgtc agcggtgacc
     1981 atcatgttat cgccaatcat cccccagacc atcaccggga tacccaccgc cagtgcgaca
     2041 attgcctgcc agcggaagcg cttcatcgta gcaacggcgg tttcctgctg gcgctcacgg
     2101 cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac cgcctgcact
     2161 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     2221 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     2281 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     2341 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     2401 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     2461 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ttgttccaca
     2521 tccgggcgct gttcgagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     2581 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     2641 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcgag
     2701 taaaagtccg taaagattaa aaaattggct caatttgcat caggattaga catttatctc
     2761 tttgttttcc tgtagttaag ctgcgggtgc taagttaaat caggatgcct caaaatcggc
     2821 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     2881 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     2941 gtcaaaatgc cgactacatt ccctttctgg cgaatgtgcc tggtcaactg gcggcagtgg
     3001 ctatcgtgac ctgcgatggc aaggtctata gtgcgggtga cagtgattac cgctttgcgc
     3061 tggaatccat ctcgaaagtc tgtacgttag cccttgcgtt agaggatgtc ggcccgcagg
     3121 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     3181 tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgcc attgccacca
     3241 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     3301 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     3361 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     3421 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc cacgctcctc aatactgttg
     3481 aactggcaac acttggcgcg acgctggcgg cggggggagt gaatccgctg acgcataaac
     3541 gcgttcttca ggccgataac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     3601 atggtcgctc cggtgactgg gcatatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     3661 gcggtattct ggcggtcgtc cctggtgtga tgggaattgc cgcgttctca ccaccgctgg
     3721 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     3781 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     3841 cctcggtcta tggaacgtcg tttccatcgg cattggggca atggtggggg cggggatctt
     3901 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     3961 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     4021 cagcaatggc ggcattatcg acttctttcg tcgcggatta ggcaacggcg tcttttcgct
     4081 ggcgctctcg ttactgtacc tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc
     4141 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc atcttatttt
     4201 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     4261 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     4321 tattgccggt gtctggtcgt tgcaaccggc gcatatttct gtctctgcgc cccccagctc
     4381 cggtgcgttc ttctcctgta tcgggataac cttccttgcc tatgcgggct ttggcatgat
     4441 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     4501 ggcaattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     4561 atcggcatta gagttagaaa aatatgccga taccgccgta gcgcaggcag cttctccgct
     4621 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     4681 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     4741 actaccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acatcattgt
     4801 cgtggtgttg attatgctga tgacggcggc actgaattta ggctcactcg ccagcgttgc
     4861 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
     4921 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
     4981 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
     5041 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
     5101 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
     5161 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
     5221 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
     5281 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
     5341 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
     5401 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
     5461 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
     5521 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
     5581 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
     5641 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
     5701 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
     5761 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
     5821 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
     5881 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
     5941 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
     6001 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
     6061 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
     6121 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
     6181 ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
     6241 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
     6301 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
     6361 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
     6421 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
     6481 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
     6541 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
     6601 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
     6661 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
     6721 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
     6781 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
     6841 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
     6901 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
     6961 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
     7021 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
     7081 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
     7141 gaacagtgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
     7201 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
     7261 ttaccggtcc ttccggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
     7321 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
     7381 tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc gatacggtat
     7441 acgataatct gatctttccc tggcagatcc gtaaccagca gcctgacccg gccatttttc
     7501 tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaaaaat atcgccgagc
     7561 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
     7621 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
     7681 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
     7741 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
     7801 aggaagcacg ctatgaactc gcataatatt actaacgaat cattggcact ggcattaatg
     7861 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
     7921 ttctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt gctgaagtat
     7981 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
     8041 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
     8101 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc tgggtcgatt
     8161 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
     8221 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
     8281 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
     8341 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
     8401 ttacctggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
     8461 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
     8521 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
     8581 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
     8641 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
     8701 gcatcacccg tacccagcgc agcgaggatc tcctgaaacg ttttacgcgt ctgaccgtct
     8761 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
     8821 ccaacctggt gcagttgcag agccagttgc gtcgccagtg cggcatcttc tggattctcc
     8881 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
     8941 atttgcgcca ccagcccctg gtagcgggtg tcctgatcct gcaacggaat ggttttcagc
     9001 accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag cagcaggccg
     9061 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
     9121 taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
     9181 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
     9241 accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc aaactgggcg
     9301 gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
     9361 gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg ttcagaccaa
     9421 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
     9481 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
     9541 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
     9601 cccggcagca ggcgcttgag caccattact gcccaggtca ccagcgttac cggatagcgc
     9661 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
     9721 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
     9781 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
     9841 ctgtggcgca gctccatgcg cagtgcgtct gaccacgcct ccagcgcata tttactggcc
     9901 gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt catcacaata
     9961 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    10021 aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga aagggggcca
    10081 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgctc
    10141 acctcgtctg ctgcgcgatc aacactttct ggtgaatcca gatcgatcaa cacgccggta
    10201 aatcccatgt tgttcatacg ctcaacatca tccggttttc ggcaacctgc cagcacatga
    10261 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    10321 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    10381 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    10441 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    10501 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    10561 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    10621 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    10681 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    10741 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    10801 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    10861 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    10921 ggcggcacgg aaggttaaca ggaccaggaa caggaagggc aaatgccagc ggaaaacatt
    10981 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    11041 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    11101 tcgggtaagt caaccttgct ggcgatcctc gccgggctgg atgacggcag cagtggcgaa
    11161 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    11221 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    11281 gaaaacgtcg agcttccggc gctgctgcgc ggtgagagta gcgcggaaag tcgtaacgga
    11341 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc ttgatcatct tccggcacag
    11401 ctttccggcg gcgaacagca acgagtggcg ctggcgcgag cctttaatgg tcgacctgat
    11461 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    11521 gacttgctgt tttccctcaa ccgtgagcat ggcaccacgt tgattatggt gacccacgac
    11581 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    11641 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    11701 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    11761 tggagaaggg cttaagccaa caaagccggg agtttatggc gggcgatcgg gcgttgcgca
    11821 gttcgcgcga agtgccgcaa gcatggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    11881 gcaagcagct gactttcgcc accatgacat ttgcaggcga cacaccgcag ctggcgaacg
    11941 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaaccaat ccccctggcc
    12001 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    12061 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    12121 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    12181 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    12241 tcggcggcaa cgagaaccag ctcgacggct atgagaaatg gttgttaccc cagcttaaac
    12301 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    12361 gctcgcagca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcag
    12421 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    12481 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    12541 tgacgctttc agccattacc ggtggggcga taggcctgtt gttcgaaaac gtgttgatgg
    12601 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    12661 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgaccg taccgcttgt
    12721 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtttggccgc
    12781 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    12841 gtggcagtat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgttgtgcg
    12901 gtgtgctggg ctggatgctg ctgaatgtac ttcgccgcat gacgctgaaa tcgctgcctc
    12961 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    13021 cattttcgct ctcctttatg ctgctggcac tgctgctggt gttgcgtggc gatctgctcg
    13081 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    13141 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcagatagtc ccggaatcgt
    13201 tttatccggt ggtgcgggcg agactgacgg cgattaacgg caagccgaca gaaggcaatg
    13261 aagatgaggc gctcaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    13321 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    13381 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    13441 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    13501 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    13561 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    13621 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    13681 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    13741 aggtgcaggt gggaatgcgc cagcgtcatc aggagctggt ggtatggcgc acactcggtg
    13801 cggggaaaaa actgctgcgc acgacattgt ggtgtgagtt cgccatgctt gggtttgttt
    13861 ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc agtgttgcag gcgaaagtgt
    13921 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcgc
    13981 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    14041 tcaggcagtt tgcggggtga tgagagtaaa taaggggcgt tctggttgaa tggaacgcct
    14101 tgtttaaatt aattc
//
LOCUS       DAZFAH010000113        14105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFAH010000113 DAZFAH010000000
VERSION     DAZFAH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.113"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..1115
                     /gene="allD"
                     /locus_tag="ACHJWB_003144"
     CDS             66..1115
                     /gene="allD"
                     /locus_tag="ACHJWB_003144"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4314727.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1137..2372
                     /gene="allC"
                     /locus_tag="ACHJWB_003145"
     CDS             1137..2372
                     /gene="allC"
                     /locus_tag="ACHJWB_003145"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4314728.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGNRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2383..3168
                     /gene="allE"
                     /locus_tag="ACHJWB_003146"
     CDS             2383..3168
                     /gene="allE"
                     /locus_tag="ACHJWB_003146"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4314729.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3396..4541)
                     /gene="glxK"
                     /locus_tag="ACHJWB_003147"
     CDS             complement(3396..4541)
                     /gene="glxK"
                     /locus_tag="ACHJWB_003147"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4314730.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4563..5864)
                     /locus_tag="ACHJWB_003148"
     CDS             complement(4563..5864)
                     /locus_tag="ACHJWB_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO4314731.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYMPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5921..7282)
                     /gene="allB"
                     /locus_tag="ACHJWB_003149"
     CDS             complement(5921..7282)
                     /gene="allB"
                     /locus_tag="ACHJWB_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO4314732.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7342..8796)
                     /locus_tag="ACHJWB_003150"
     CDS             complement(7342..8796)
                     /locus_tag="ACHJWB_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4314733.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(8965..9843)
                     /gene="glxR"
                     /locus_tag="ACHJWB_003151"
     CDS             complement(8965..9843)
                     /gene="glxR"
                     /locus_tag="ACHJWB_003151"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4314734.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9943..10719)
                     /gene="hyi"
                     /locus_tag="ACHJWB_003152"
     CDS             complement(9943..10719)
                     /gene="hyi"
                     /locus_tag="ACHJWB_003152"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4314735.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10732..12513)
                     /gene="gcl"
                     /locus_tag="ACHJWB_003153"
     CDS             complement(10732..12513)
                     /gene="gcl"
                     /locus_tag="ACHJWB_003153"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4314736.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLSFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(12603..13418)
                     /gene="allR"
                     /locus_tag="ACHJWB_003154"
     CDS             complement(12603..13418)
                     /gene="allR"
                     /locus_tag="ACHJWB_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4314737.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13496..13978)
                     /gene="allA"
                     /locus_tag="ACHJWB_003155"
     CDS             complement(13496..13978)
                     /gene="allA"
                     /locus_tag="ACHJWB_003155"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4314738.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         3719 a         3765 c         3291 g         3330 t
ORIGIN      
        1 tccagatatt ttccctgtat gttaattgca gtcatgcttc acaccgtcgt taaaaaggaa
       61 gacagatgaa aatcagtcgg gaaacactcc accagctaat tgagaataaa ctctgccagg
      121 ctgggttaaa acgtgagcac gctgcaaccg tggctgaagt attggtttac gccgatgcca
      181 gagggatcca ctctcatggc gcggtgcgcg tggaatacta cgcggaacgc atttcaaaag
      241 gcggcaccaa ccgcgaaccg gagtttcgtc ttgaggaaac cgggccgtgc tcggcaattt
      301 tacatgccga caatgccgcc ggacaggtcg cggcgaaaat gggtatggaa catgccatca
      361 aaaccgccca gcaaaatggc gttgcggtgg tcggtatcag ccggatgggt cacagcggcg
      421 caatctctta ttttgtgcag caggcagccc gcgccggatt cattggcatt tcgatgtgcc
      481 agtccgatcc aatggtggtg ccgtttggcg gcgcggaaat ttactacggt actaaccccc
      541 tggcctttgc cgcgccggga gaaggcgacg agatccttac ctttgatatg gcgactaccg
      601 tacaggcatg gggaaaagtg ctcgacgccc gctcgcgtaa tatgtctatc ccggatacct
      661 gggcggtcga taaaaacggt gcaccaacaa ccgatccgtt cgcggtacat gctctgctcc
      721 ccgccgccgg gccaaaaggg tatggcctga tgatgatgat tgacgtcctc tcaggcgtct
      781 tactcggctt accgttcggg cgacaggtta gttcgatgta tgacgattta cacgcagggc
      841 gtaatttggg gcaattacat atagttatta atccgaactt tttctcctcc agcgaattat
      901 tccgtcaaca tcttagccag accatgcgcg aattaaatgc cattaccccc gcgcccggtt
      961 ttaaccaagt ttattatccc ggacaggatc aggatattaa acaacgcaaa gccgccgtcg
     1021 aaggcatcga aattgttgat gatatttacc agtatttaat ttccgacgcg ctttataaca
     1081 cgtcatacga aacgaaaaat ccctttgcgc aataattatt gagacaggac tttcttatga
     1141 ttacacattt tcgtcaagcc atagaagaaa cgctgccctg gctttcctct tttggcgctg
     1201 acccaacggg tgggatgacc cgtttacttt attcgccgga atggctggaa acccagcagc
     1261 aatttaaaaa aagaatggca gcaagtgggc tggaaacacg tttcgatgaa gtagggaatt
     1321 tatacggtcg cctgaatggt accgaatatc cacaggaagt gattctgagc ggttcgcata
     1381 tcgataccgt ggttaacggc ggtaacctcg acgggcaatt cggcgcgctg gcggcgtggc
     1441 tggcaattga ctggctgaaa acgcaatacg gcgcgccgct acgtacggtc gaagtggtgg
     1501 cgatggcaga agaagaaggc aaccgcttcc cgtatgtctt ctggggcagt aaaaatatct
     1561 ttgggctggc gaatcctgac gacgtgcgga atatctgtga tgccaaagga aatagttttg
     1621 tcgatgcgat gaaggcttgc ggatttactc ttccgaacgc cccactaact ccgcgtcagg
     1681 atattaaagc ctttgttgaa ctgcatattg aacagggctg tgtgctggaa agtaatgggc
     1741 aatcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg ttatacggta acgctgaacg
     1801 gcgaatcaaa ccatgcaggc accacgccga tgggttatcg tcgtgataca gtttacgctt
     1861 tcagtcgcat ttgccatcag tcggtcgaaa aagcgaaaag gatgggcgat ccgctggttc
     1921 tgacctttgg caaagtagag ccgcgcccga atacggtaaa tgtggtgccg ggtaaaacca
     1981 cgttcaccat tgattgtcgt cataccgacg ctgccgtgct gcgcgatttc acccaacagt
     2041 tagaaaacga catgcgggcg atttgcgatg aaatggacat tggtattgat atcgatttat
     2101 ggatggacga agaacccgtg ccgatgaata aggagctggt cgccaccctg acagaattgt
     2161 gtgaaagtga aaaactgaat taccgggtga tgcacagtgg tgccgggcac gacgcgcaaa
     2221 ttttcgcgcc tcgcgtacca acctgcatga ttttcattcc cagcatcaac gggatcagcc
     2281 ataacccggc ggaacgcacc aatattaccg accttgccga aggggtcaaa acgttggcac
     2341 tcatgcttta tcaacttgcc tggcagaaat aaggagtcat aaatgggata tttaaataac
     2401 gtcaccggtt accgcgatga tttactggct aaccgtgcga ttgttaaaca cggtaatttc
     2461 gcactgttaa ccccggatgg tctggtaaaa aatattattc cgggctttga aaattgtgac
     2521 gcgacaatcc tctccacgcc aaagctgggt gcctcttttg ttgattatct ggtcacactg
     2581 catcaaaacg gtggcaacca acagggcttc ggtggcgaag gcattgaaac gttcctgtat
     2641 gtgatctctg gaaatatcac tgccaaagcc gaaggcaaaa catttgcctt aagcgaaggt
     2701 ggctatcttt attgcccacc aggctcctta atgacgtttg ttaacgccca ggccgaagac
     2761 agccaaatct ttttatataa gcgccgctat gttccggtag aaggccatgc accgtggctg
     2821 gtttctggca atgccagcga actggaacgc attcactatg aaggtatgga tgatgtgatc
     2881 ctgctggatt ttctgcccaa agagttaggt tttgatatga acatgcatat cctctctttt
     2941 gcaccaggtg ccagccacgg ttatatcgaa acacatgttc aggaacacgg tgcctatatt
     3001 ctttccggtc agggggttta taacctcgac aataactgga tcccggtgaa aaaaggcgat
     3061 tacatcttta tgggcgctta ttctttacag gctggttatg gtgtagggcg tggtgaagcg
     3121 ttcagctata tttactcgaa agattgcaac cgcgacgtag agatttaagt cataccactc
     3181 gcctgattat ttttagctat gttggatttt gccacggttt ataccgtggc tttttttgta
     3241 acattgcctg atgcgcttcg cttatcaggc ctacatgatc tctgcaatat atagaatttg
     3301 cgcgattttc aggaaggata tagtactcat gccgcatccg gcatgagtac tgcactctta
     3361 tgcgatataa atcgcatccg ctttaaaggt aagggtcagt ttttaattcc ctgacctatt
     3421 ttaatggcgc aggcaatatt tcgtgcgctg ttgaagagat tggtttcacc gctggcaagt
     3481 acttcggcta aaggtgccag acgtggcaaa atgctgaata ccgcatcaat gccgtactgg
     3541 tgcaccactt ccacgccatc acccaatacg ccagcaatcc caatcaccgg tacattaaac
     3601 tgcttcgcca ccgacgccac acccagcggc gctttaccgc ctgccgtttg cgagtcgatg
     3661 cgcccttccc cggtgatcac cagtgctgcg ccctgcactg cctgcgcaag attgacagca
     3721 ttcaacacaa tctcaatacc tggtttaata tccgcattga gaaataccgc cgccgcaata
     3781 cccataccgc ccgcagcgcc gccgcccgcc atctggcaga cgttgatttc agtttgctgt
     3841 tgtaaaacac gggcgtaatt ttgcagcccc tgttccagct cttcgaccat ttccggcgtt
     3901 gccccttttt gcgggccaaa caccgccgcc gcgccgcgtg cccctaccag cgggttatca
     3961 acgtcacatg ctacttcaat atggcaattc gccagacgcg gatcgcattc atccatctca
     4021 atgcttgcca cgcgcgctaa attaccgccg ttagccacca gcacctgacc gtcggcatca
     4081 aggaaacgca caccgagcgc ctgcgccatg cccataccac cgtcgaccgt cgcactgccg
     4141 ccaatgccga gaataatatg gcgaatgccg ttatccagcg catgacgaat taactccccc
     4201 gtaccaaaac tggaggccag caacggatta cgcttttcag gcgcgaccag catcaggcca
     4261 cttgctgccg ccatctcaat caccgccgtt ttcccgtcgc cggtaaggcc ataaaaagca
     4321 ttcacttttt cgcccatcgg cccgcagact tcaagcgtca cgatgttgcc gcccgtcgcg
     4381 gcgaccatcg catccaccgt tccttcgccg ccatccgcta tcggcaaaca gatatagttc
     4441 gcatcgggaa agagggtcga aaacccggct ttaattgcct gacaacattt ttctgcactt
     4501 aagctctctt taaaagagtc tggcgcaatg acaatcttca ttatcaatcc tttttcaaca
     4561 aattattcga tacgttccca cggaataagg ttatccataa gaacagccag taaaatcccg
     4621 accaataagc cgttactgag cagaggacga agcgttaatg gaaggtcttg cagatacaca
     4681 ggcggtaatg ccattaaaaa tatgccgaca aataacggca atgcgagtcg ataaatattg
     4741 cgagcggtaa aggttatttg ctggctaaac accagcgcgg aaaagagtaa aggcatataa
     4801 gaaaccagca tgaccgcgct actcacgggt aaagggatac tgcaaaacag tcgtgtgagt
     4861 gccggaacca gcgccaccag caggcaaata acgctgccat aaataaacga acgccgcgtg
     4921 taatcgcctg tctgggttaa taaaccaatg gatgaaacaa acggtgaaaa tggaattacc
     4981 gccagcggta cggttatcag cgtcataaat ccggtcgcca caaagctgcg acgataacgc
     5041 gtatttcccg cgccctgctg cggataaaaa acatccgtgc cccgaatcgc accgtaggta
     5101 ttgctgatat tcaccagacc tgtaatcacc gccgtcagaa taattcccgg agacaaagca
     5161 cctccactgc cgagcgggaa ccactgccag tgcaactcac cggagagcga gtgcgaagaa
     5221 ggaaagcaaa agtaccacaa caaccagccg gttatggtgc cgaccagcag gccataacgg
     5281 gcaaaacgtt gcggcaggaa gataatcatc cccagtacca ggcacatcac cgccaccgag
     5341 agcgcgaacg gcggtaactg aattttaaaa tttgggtcgg ctatgccaaa cggcagcccg
     5401 agcatacctt tgaaaaagat agtggtcagc tgcgcgccca gcatcaacat aaacaagacc
     5461 atcaccgacg gcgtaaataa ccgtgccagg cgatggccta atccgctaaa accaatcaac
     5521 atcgtcagca cgccggagag cgcaatcccc accgccaggc tggtagcgat atcgttgatc
     5581 ggtgtcccgc gcgatgcttc gccaagggtg atagtaagga tggttcccca ccacaggcca
     5641 cccggccctt ccataatagc gcgacgatga ccgcagaacg cctgagcgaa gcaggccagt
     5701 gcggtagcaa gaaaagcata ttgcgtgagc gtaagcaggc tactttgcgg caactgaaaa
     5761 gcagaaagta gcgtaggagg aaccacaacc gtgttgcaaa aaataaagaa aaaccactga
     5821 aatcctgata acaggctttc gcggctgact gcaaaattga acataaacac accttaaccg
     5881 gagaatgccc gccgcaagga cgggcattgc aggggccaga ttactgctga tgtttaagga
     5941 taaattgacc tttcggcgca acagggaagc cctgttcaat gtcgtaaatc acatcaccac
     6001 gtaagatggt tttcgtgata cgcgcgccaa tggtacggcc aacatacggg ctgactttgt
     6061 ggcgatattc cagatcgtca ttggtaagaa catagctgct attcggctga atgaagacga
     6121 agtcggcatc ttttcctggg gcgatacggc ctttttgctg cagaccgaaa atatctgctg
     6181 cgttagtcgc cattaatttg ccgaacattg gcagagacat tccgcgtttc tgtaccgctt
     6241 catcgaacat cacgtccata cagttttgca gaccggcaat accgccccat gcttccatga
     6301 tgttgccggc tttcatttcc ggagggcatg gtgagtggtc ggaaaccagg cagtcgattt
     6361 caccgttaaa cagtttttcc cacatgcctt tctggttttc cagatcgcgg atcggcggtg
     6421 aacacttcgc cagagtgccg atttcttcga actgatcggt atccagtaca aagtaatgcg
     6481 ggcaggattc acaagtaacg tcctgacctt cctgacgtgc acgagtcact tcttcaacgc
     6541 cttccgggct gctgatatgg caaacgtgca gacggcaacc ggcaactttc gccagataca
     6601 gcacgcggcg aatcgcttcc acttcggtaa ataccggacg cgaagccaca tagtcatggg
     6661 cagttacgcg accttcacgt ttcgcttctt cgcccagtgc gtcacagatc agcgcgtttt
     6721 cgcagtgcac cagcaccggc tgccccagtt cgcccagctt ctgcgcacct ttgaagaact
     6781 gccagtcgtt tacatcacgg aagtcgttgt cgataccgcg atcgccacag gtcgcaacga
     6841 agcatttgaa gccgacaacg cccacttcat ccagctcatg cagacggtcg atgttgtaag
     6901 acaccaggcc accgagttgt gccgcatcga tagtcagctt gcctttagcg gcatcgaact
     6961 tcagttcaat tgaagcgcgg tcaaccgttg caggcagctg gttgagcggc atttcgatca
     7021 tggtggtgat accacctttt gccgctgcgc gagtaccggt ttcataacct tcccagtggc
     7081 tacgacccgg ttcagaaata tgggtgtgcg catcaaccat gcccggcgaa accaccagac
     7141 cagacgcatc cataacttct tttgcatcgc ccagatcctg accgatagca gcaattttcc
     7201 cgcctttaac ggcgatatct acaacgcgag cttcgttttc taaaataacg gtgccgtttt
     7261 taatgattaa atcaaaagac ataacaaact ccttgtaaaa tacagattgg tttttaactc
     7321 aatgttcaat atcgggatta attaacctat ggttttttgc tctcctgttt tttctgctgt
     7381 tgtacgtttc tttaataagg cgtaggccgc aaaggcgacg atgacgccga caaaccatga
     7441 aacacgcgat aacggttcca taaagggaat aaacttaccg ccaagagata aaataacggc
     7501 caccagagtt actgaaaacg cagtgaggtt aaaaccgtta tcgtaatatt tataatcgcc
     7561 aggtgcggta tacagttcat caagattaat tttcccacgc atcaccacaa aataatgcgc
     7621 catcatgaca ccaattaccg gaccaagcat tccgccgata atatcgagga acagataaat
     7681 actgtcctga ttttccatta atttccacgg gcagatcagc aggctgataa tactggcaat
     7741 cagtacgccg tttttatagg tcagtttcgt cggtgcaata gcggcaatct gataaccggc
     7801 aggaataata ttaccggtgg cgttagtgga gatagtcgtc atcagaataa ccagtaccgc
     7861 aaagaacgag gcgaacaagc tgtcccaacg ctgaacaata tccagcacgt tccaggtatc
     7921 agcgccgtaa tgaatactgg ctccggcaat aatacatacc cccgcgaccg caaacagaat
     7981 ataggccaca actaaaccca gcgtttgccc cagcgcttgc tcacgaaacg agtgggcgtt
     8041 ttgcgtaaag tcggatgcgc tcaccgccgg tgccgcccag accgcaacta ccgcgtttat
     8101 caccaccagg aacaggaagc cactgttttc tgctttctga ataccgctcg gaatgtagtc
     8161 aaagattgga ccgagcccga ccagcgaaat cgcccaaatc gccataccgc cgaaaacgat
     8221 atagatgcac gggttaagaa tggcagtgaa tttatttaaa actttgccac cgccaaaacc
     8281 tataccaacg ttgaccagcc agaagagtaa gaaagtaatt aagcccggta gagaaagccc
     8341 taacagcgtg aaatcaccac cgagagttaa aaatcccggc cagattttgc caatcagaat
     8401 caagcaggcc agtgaccccg cgtaacattg caggccaaac cacataatgg cagcaatacc
     8461 gcctcttaat aatcctggaa acagtgctcc acgtacgccg taagaagcac gcaggatcat
     8521 ggcaaacggt acgccgtatt tactgcccgc agcaccgttt aataccatta ccgcggcaat
     8581 gaaaaaggcg ctgaggataa ttgccaacat aatactaaag gtagacaagc cgagaataaa
     8641 aaagccgccg accatcacat aattgggaac gttatgaacc gaacccatcc ataaggtaaa
     8701 atagttaaat gttttccagg tccgctggct ttgcgttttc ggcaatagat cttcgctata
     8761 gccgcgttgc tggaatagtt ttctctgatg ttccataaat ccctcttaat ttcacgtatc
     8821 gaataacgtt gttttaatct agctatgagg aacaaataaa ataaaaatat tatttttaat
     8881 actgaagcaa catctttggc tgctttcttt tttaatattg ctggccgatt catattctga
     8941 tatcggccat ttttattgcg ggtatcaggc cagtttatga ttcgccatta attccagcgc
     9001 ctgcactaac gcagagtgat ccaactggct gccaccgttt gccgcacagg tattaaataa
     9061 ctcctggcag gtcgcggtgt ttggcagatt cagcgcaagt gctttcgcac tttgcagtgc
     9121 caggttgaga tctttctggt gcagcgcaat tttgaaacct ggattaaagg tgcgtttaat
     9181 catgcgctca ccgtgaactt ccagaatacg tgaggaagca aagccgccca tcagcgcctg
     9241 gcgcacacgt accgggtctg caccggcttt tgaagcaaat agcagggctt cagaaaccgc
     9301 ttcaatattg agcgccacaa taatctgatt tgccactttg caggtttgac catcgccgtt
     9361 accgcccacg agggtgatat ttttaccgag cagttcaaac agcggtttaa cacgttcaaa
     9421 taccgcttca tcaccgccaa ccataatcga caacgtccct tcacgcgcac cgatttcacc
     9481 gccggagact ggcgcatcga gataatcgcc gcccagttca ttcacctgac gagcgaaacg
     9541 cttagtttca atcggggaaa tggagctcat atcaacaatg gttttgccct tcagcgaggc
     9601 tttggtacaa ccattttcac cgaacagaac ttcttcaacc tgaggtgtgt ccggcaccat
     9661 aataaaaatg atgtccgatg cttccgttac ctggcgagca gtttcaacac tgacggcacc
     9721 cagtgacagt aattcatcag caaccggtcc aatggtcgtg acatgtaatt gatgaccggc
     9781 acgcgccaga ttaatggcca tcggtgtacc cataatgcct aagccaataa atcccagttt
     9841 cataattaac ctcttttaaa tttcgctttt cctgaattca gacaacacga tcccctgcca
     9901 acttagcggc ctaaaaaagc attgtctgaa tagcgttacg ttttaacggt acggatccat
     9961 ccagcgtaaa ccggcttccg tggtggtttg gggtttatat tcacacccaa cccaaccgtt
    10021 gtagtcagaa ttttcgatta ccttaaagag ataatcataa ttaatttctc cggttcccgg
    10081 ttcgccgcga tgcggattat cggcaatttg caggtgacca attttatcag cccactgagt
    10141 catggtgttg gttaattcac cttccatccg ctgcatgtga taaatgtcat actgaatttt
    10201 caaattgcag caaccaacat catcaatcaa tttcagcgcc tgccgagttc cggtgagatg
    10261 gaaaccagga atatcaaaat ggttaatagg ttcaatcagt aataaaatat cttctttcat
    10321 cagcatattc gcggcataac gcaggttttc tacaagcgtt gcgtgaatct gttcactgct
    10381 gaaaccagcc ggcgttttac cgaccagaca gttaattttt ttattaccca gcgcacgggc
    10441 ataacgaatc gctgctgcta cgccatcccg aaactcttct tcacggccag gaatacaggc
    10501 aataccgcgc tcccccgccg cccagtcacc cgccggtaaa ttgtgcagcg tatgttcgag
    10561 tttattactc gccagcacct gttttaattc ttcaatgtcg tagtcataag gaaacataaa
    10621 ttcaacgccg cgaaaaccac actgcgcagc tttctcaaaa cgggcgagaa aatcatattc
    10681 tccaaataac atcgataaat tagcagagaa acgtaacatt atttatctcc cttattcata
    10741 gtgcatgaag caggtttcag tcggtgcgtc cgctgcgtta tcggcgatat cttcaaattc
    10801 catgacgtta tccagttcgc tgcccatcga aatattggtc acacgctcga gaataacttc
    10861 cacgactacc ggtacccgat attgcgccat taaggctttc gcctgttcaa aggctggcgc
    10921 aatatcttcc ggtttgaaga cgcgaatcgc tttacaacct aaaccttccg ctacttttac
    10981 gtggtcaaca ccgtagccat tcacttcact ggagttgata ttctcgaaag cgagttgcac
    11041 gcagtagtcc atatcaaacg cgcgctgcga ctggcgaatc agccccagat aagcgttatt
    11101 gaccagtaca tggatgtacg gaatgttgaa ctgtgcgcca acggccaatt cttcaatcag
    11161 gaactggaag tcgaagtcac cggaaatcgc caccactttg cgctctggat cagcggcaca
    11221 aacccccagc gccgccggaa tggtccagcc cagcgggcct gcctgcccac agttgatcca
    11281 gtggcggtct ttaaagacat gcagcatttg cgcagcggca atttgcgaca gaccaatggt
    11341 ggtgacatag caaacatcac gtccaaaagc tttgttcatc tcttcataca cacgctgcgg
    11401 tttaaccgga acgttgtcga agtgtgtttt acgcaacaaa gtacgtttgc gctgctggca
    11461 ctcagcaacc cactctttac ggcatggcag acgccctgct ttttgcattt cctgcgccac
    11521 ttcaaccagc agtgtcagcg ccgctttagc atcagagaca atccccagat ccggacacag
    11581 cacgcggcca atttgcgtcg gctcaatatc gatatgaacg attttgcgcc cttcggtgta
    11641 tttctctacc gaaccggtat gacggttagc aaaacggtta ccgataccaa acaccatgtc
    11701 ggacgccagc aacgttgcgt taccgtaacg atgcgcggtt tgcagaccca ccatcccggc
    11761 catcagttca tgatcgtccg ggatacagcc ccagcccatc agcgttggga tcaccggaac
    11821 gctggtcagt tcagcaaact gttgtaacag tgcagctgcg tcagcattaa ttaccccgcc
    11881 cccggcaaca atcaccggac gtttggcctg gattaacatt tctacagctt tttcgatctg
    11941 catacggctg gcagcaggtt tgtagaccgg cagcggttcg tacatatcag gatcaaactc
    12001 gatttccgca acctgaacgt cgaacgataa atccaccagt accggacccg gacgaccaga
    12061 acgcatcagg tgaaatgcct gttgcagcac acgaggcacc agcgccgctt cacgtactgt
    12121 aaccgccatt ttgctgaccg gcttggcgat agcttcgata tcaacagcct ggaaatcttc
    12181 tttatgcaga cgggcgcgcg gagcctggcc ggtaatacac agaataggaa tggaatcagc
    12241 agaagcagaa tagagcgcgg tgatcatgtc cgtgcccgca ggaccggaag tccccagaca
    12301 tacgccgata ttccctgccg ttgcgcgggt ataaccttcc gccatgtgcg aagcaccttc
    12361 cacatgacgc gccagaatgt gacgaatacc gccgtgctta cgcatcgctg agtagaacgg
    12421 atttattgca gctcccggaa cgccgaaggc ggtggtgata ccttctttct ccagcacata
    12481 cattgccgcg tcaacggctc tcatttttgc cattttattc ctacctctat taattggaaa
    12541 atttttccaa ctttcaaatt ttgtcaaaaa aacccgctcc gcagagcggg atgcgacaga
    12601 cattatggat gtgctttcag tcccaacgcc gtgctgatat cgcgggcggt gtctctgacc
    12661 agctcaccct gactgacaaa acgatcttct gtcagtcttg atgaaggccc ggagatggag
    12721 atagcggcaa caacactacc tacatcatcg taaattgctg aagctaagca attcagacct
    12781 acaacatgct cttctttatc tacggtatag cccagttcac gcgcttgttc caggtccttc
    12841 agcaaggtgg gcatatccac aagcgtagtt ggcgtaaact gctgcaaacc ggtttgcaga
    12901 atgatgctca tcaactcctc ttccgccagc ggataaagca gcgctttgcc cgcaccggaa
    12961 gcatgcagcg gcagacgact gcccagtggc gcacacatcc tgaccattga tttacactct
    13021 aactgaccaa ttaatactgc ttcattgccg ttacggatcg cgacattgac cgtttcgccg
    13081 gaaagtaaca tcaggcggcg cataaacggc ccggcgacgg agaggacatc gcggttatgg
    13141 atgtacgccg caccgacgtt aaagacacct aatcctatat gccaccagcc taattggctg
    13201 tcctgataga caaaatccgc tgcttgtaaa accttcagca agcgaaaggt cgtggagagc
    13261 ggcaaatcca gattgagaga aatatcgcta accgacgagc ttcccccact tttttccaga
    13321 tattgcagaa tcgcaattcc ccgctctaac gcctgtgcgc ccttctgtgc cacaggctcc
    13381 gcctgtcctg gcctgccgcg ccgtctaact tccgtcatac ctacgttaac tccgacctac
    13441 aagtgagcat aaacaatgaa caattagttt atcttatgca agtcggttgc aggcgtcacg
    13501 caaaacagag ttcctgttca ggaatacttt caacatcaca gttgtcactg ccgccgcgat
    13561 cgatggtcag aaaatcggtg acgcgctgcc aggcgaaaag tgggtgatgc cagacgttac
    13621 gatggtaatt cactccctgt tcgccgttgg tgataaacgc ccgcagcgtt gacaggtctg
    13681 gtttgtcgtc gcctaacgcc acgaccacca caaacacttc acctttcatc gggataaagg
    13741 cctgagtgcc cagcggatgg cgttcgagtt cgtgaatggt cagcggcaga ttcgccggtt
    13801 gcgcgcggtt aatgctgata agcgtacggt cttgctcaag aatctcaacc agcgccaaat
    13861 cgtggtaacg ctccaccagg ccattgttaa tatggaaaaa atcccgttgc tgcgtttcga
    13921 ttacgtcgcc ataagcacta aaggcttcct gacttaacgg taatacctga agtttcatct
    13981 ttttgtcctt gtaacataat tccgttcaac caccgccgac cggtcagctt ggtttcaata
    14041 acaggcaatc taaaacctta agagaacaac atcaatacaa aagttggaat atttttccaa
    14101 aacta
//
LOCUS       DAZFAH010000114        13885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFAH010000114 DAZFAH010000000
VERSION     DAZFAH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.114"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..857
                     /locus_tag="ACHJWB_003156"
     CDS             <1..857
                     /locus_tag="ACHJWB_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4314739.1"
                     /translation="KGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIA
                     LESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTD
                     NQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLP
                     LYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKTREATP
                     GKEIEAITRRILDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQST
                     SKTAPQAK"
     gene            complement(907..1260)
                     /locus_tag="ACHJWB_003157"
     CDS             complement(907..1260)
                     /locus_tag="ACHJWB_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4314740.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1398..3044)
                     /gene="groL"
                     /locus_tag="ACHJWB_003158"
     CDS             complement(1398..3044)
                     /gene="groL"
                     /locus_tag="ACHJWB_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4314741.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3088..3381)
                     /locus_tag="ACHJWB_003159"
     CDS             complement(3088..3381)
                     /locus_tag="ACHJWB_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4314742.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3657..4913
                     /gene="yjeH"
                     /locus_tag="ACHJWB_003160"
     CDS             3657..4913
                     /gene="yjeH"
                     /locus_tag="ACHJWB_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4314743.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4929..5405)
                     /locus_tag="ACHJWB_003161"
     CDS             complement(4929..5405)
                     /locus_tag="ACHJWB_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4314744.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5742..7178
                     /gene="aspA"
                     /locus_tag="ACHJWB_003162"
     CDS             5742..7178
                     /gene="aspA"
                     /locus_tag="ACHJWB_003162"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4314745.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7296..8597
                     /gene="dcuA"
                     /locus_tag="ACHJWB_003163"
     CDS             7296..8597
                     /gene="dcuA"
                     /locus_tag="ACHJWB_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4314746.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8713..9051
                     /gene="cutA"
                     /locus_tag="ACHJWB_003164"
     CDS             8713..9051
                     /gene="cutA"
                     /locus_tag="ACHJWB_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4314747.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9027..10724
                     /gene="dsbD"
                     /locus_tag="ACHJWB_003165"
     CDS             9027..10724
                     /gene="dsbD"
                     /locus_tag="ACHJWB_003165"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4314748.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10761..11336
                     /locus_tag="ACHJWB_003166"
     CDS             10761..11336
                     /locus_tag="ACHJWB_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4314749.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11443..11518
                     /locus_tag="ACHJWB_003167"
     tRNA            11443..11518
                     /locus_tag="ACHJWB_003167"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11476..11478,aa:Phe,seq:gaa)
     gene            11716..12978
                     /locus_tag="ACHJWB_003168"
     CDS             11716..12978
                     /locus_tag="ACHJWB_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4314750.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGASMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDKARKLISEGIDPCEQKRTKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRKHRPALDLSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRRPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKFISPFEYAKINNPLK"
     gene            complement(13178..13860)
                     /locus_tag="ACHJWB_003169"
                     /pseudo
     CDS             complement(13178..13860)
                     /locus_tag="ACHJWB_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         3421 a         3555 c         3375 g         3534 t
ORIGIN      
        1 tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca ctggcgctga
       61 aaattaaaga acctcgcaat aaagaatcgt tgttctttat gtctgtcatg gaactgcgcg
      121 acctgctgat tgcgctggaa agtcgtctgc atcaaaagca taaactggat gctgctgcac
      181 gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa aacatcccag
      241 aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac acgctggaac
      301 ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac ggtagcaaat
      361 gtgagttagt cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc attcatgcaa
      421 ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta gatttcctgc
      481 cgttatacga cgttgactgc caggacaatg gcaatctgga atatgacacc tattcccaac
      541 cggaatggaa acataacctg ttcgaccatt atctggcggt gctttaccgc tttaaagatg
      601 aaagtggcaa agaacaattt agcggcacag tggtgaaaac gcgtgaagca acgccgggta
      661 aagaaataga ggcgatcact cgccgaatac ttgatttcag cccacgactg aaaaaactcg
      721 cgggtgtacc atgtcaggtc tatgttcgca ccgtagcggc aaataacgcc caaccgctaa
      781 cccaggatca atgcttacgc gcgctgcatc acttacgtgt tcaatccacc agcaaaactg
      841 caccacaggc gaaataatct catctgtttt actacgaggc tggaagtatc cagcctctgg
      901 atcacatcag ttcggacact tatatacctg gccgataatc tggctatccg tcgggacaaa
      961 actggacaac attccctgcg acgggctgct gatgccataa atcacgttac cgcccattgc
     1021 agccgcctgg ttgcgcagat cgtttgctgc gccgcgcata gaaccacctt cttctccgtg
     1081 ttgcccggaa agccagttgc tttgcttacc tgtcgcagta ccaatcagct ggcactctgc
     1141 gcctggctgc tcgtccacaa tgcgtacact ctgaccggca gcactcaatt cgttgctgga
     1201 gctacaaccc gccatcagta gcgcggcacc gacaatccct gctaagtatt ttacgtgcat
     1261 gttattcccc ataatcaatg agctggacgc cactcgcgtc gtccgtgtct gaatcttata
     1321 ctagaaagat gaccaaaaga aaaaccccca gacatttctg cccgggggtt tgtttatttc
     1381 tgcgaggtgc agggcaatta catcatgccg cccatgccac ccatgccgcc cataccgcca
     1441 gcagcgccta agtcagctgc atcgtttttc ggcaggtcgg taaccatgca ctcggtggtg
     1501 atcatcaggc cagccacaga agccgcgtac tgcagagcag aacgggttac tttggttgga
     1561 tccaggatac ccatgtcgat catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag
     1621 ttgccgtcgc cgcctttaac ggtgttagca acaacagacg gttcttcgcc gcagttcagg
     1681 acgatctgac gcagcggagc ttccattgca cgcagtgcaa ctttgatacc cacgttctgg
     1741 tcttcgttct gaccacgcag gtcagccagt ttagacgcta cgcggatcag cgcaacacca
     1801 ccaccagcaa ccacgccttc ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg
     1861 cgtgcttttt tctctttcat ttcaacttcg gtagcagcac ctactttgat aactgcaacg
     1921 ccgcctgcca gtttcgctac gcgttcctgc agtttttcac ggtcgtagtc agaagttgct
     1981 tcttcaatct gctgacggat ctgagcaaca cggccctgga ttgcagcttc ttcacccacg
     2041 ccatcgatga tagtggtggt gtctttgttg atcacaacac gtttagcctg acccaggtct
     2101 tccagggttg ctttttccag ctccataccg atctcttcag agattacggt accgccagtc
     2161 agggttgcga tatcctgcag catagcttta cgacgatcgc cgaagcccgg agctttaacc
     2221 gcagcaactt tcacgatgcc acgcatggtg ttaacaacca gagttgccag cgcttcgcct
     2281 tcaacatctt cagcgatgat cagcagcggt ttgcctgctt tggcaacagc ttccagaacc
     2341 ggcagcattt cgcggatgtt agagattttc ttgtcagcca gcaggatgaa cgggctttcc
     2401 agttctactg cgccagtttc cggcttgttg atgaagtaag gagacaggta gccacggtcg
     2461 aactgcatac cttcaaccac gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg
     2521 ataacgcctt ctttaccgac tttgtccatc gcttcagcga tcagtttacc tacggtttcg
     2581 tcggagttag cggagatggt accaacctga gcaatcgctt tagagtcaga gcacggtacg
     2641 gacagcgctt tcagttcttc aactgcagcg gtaacggctt tgtcgatacc acgtttcagg
     2701 tccatcgggt tcatgcccgc agcaacagct ttcagaccct cagtgatgat agcctgagcc
     2761 agtacagttg cagtggtggt accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct
     2821 ttcaccatct gcgcacccat attttcgaac ttgtcttcca gttcgatttc acgagcaacg
     2881 gaaacaccat ctttggtgat ggtcggtgca ccgaaagatt tatccagaac tacgttacgg
     2941 cctttcggac cgagggtaac tttcactgca tctgccagta cgtttacgcc gcgcagcatt
     3001 ttcacacgag cgtcgttacc gaattttacg tctttagctg ccattatctt tattccttaa
     3061 attcgtatgt tcagtgtcgt gcgaggatta cgcttcaaca attgccagaa tgtcgctttc
     3121 ggacatgatc aacacttctt cattgtcgat cttctcagat ttcacaccgt agccatcgtt
     3181 gaaaataacg atgtcgccaa ctttcacatc cagcggcttc acttcgccat tttcaaggat
     3241 acggccattg ccgacagcca gcacttcgcc gcgggtggat ttagccgctg cagagccggt
     3301 cagaacgatg ccgccagcag atttagtttc aacttcttta cgcttgacga tcacgcgatc
     3361 atgcaatgga cgaatattca ttgataactc tcctttgaga aagtccgtat ctgttatggg
     3421 tgacgccgga gcttacgtgg tttcccggcc ggtgaccaga gaaatgggga tgaggattcg
     3481 ccccttcaag ggggaaaaag aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct
     3541 gattcacctc acatttcatc gcaatttctt catcgccgtc ataagcgaat ctgattgtgc
     3601 taccatcgaa aatctacgca gttgcccaaa atttgggcgc aatcggatat caacccatga
     3661 gtggactcaa acaagaactg gggctggccc agggcattgg cctgctatcg acgtcattat
     3721 taggcaccgg cgtgtttgcc gttcctgcgt tagctgcaca ggtagcaggc aataacagcc
     3781 tgtgggcgtg gcccgttttg attatcttag tgttcccgat tgcgattgtg tttgcgattc
     3841 tgggtcgcca ctatcccagc gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt
     3901 cgcggcttga gcgagtcacc ggctggttgt ttttatcggt cattcccgtg ggtttgcctg
     3961 ccgcgctaca aattgccgcc gggttcggcc aggcaatgtt tggctggcat agctggcaac
     4021 tgttgttggc agaactcggt acgctggcgc tggtgtggta tatcggtact cgcggtgcca
     4081 gttccagtgc taatctacaa accgttatag ccggacttat cgtcgcgctg attgtcgcta
     4141 tctggtgggc gggcgatatc aaacctgcga atatcccctt cccggcacct ggtaatatcg
     4201 aactgaccgg gttatttgct gcgttatcag tgatgttctg gtgttttgtc ggtctggaag
     4261 catttgccca tcttgcctcg gaatttaaaa atccagagcg tgattttcct cgcgcattga
     4321 tgattggtct gctgctggca ggattagtct actggggctg tacggtagtc gtcttacact
     4381 tcgacgccta tggtgaaaaa atggcggcgg cagcatcgct tccaaaaatt gtagtgcaac
     4441 tattcggtgt aggagcgtta tggattgcct gcgtgattgg ctatctggcc tgctttgcca
     4501 gtctcaacat ttatatacag agcttcgccc gcctggtctg gtcgcaggcg caacataatc
     4561 ctgaccacta cctggcacgc ctctcttctc gccatatccc gaataatgcc ctcaatgcgg
     4621 tgctcggctg ctgtgtggtg agcacgttgg tgattcatgc tttagagatc aatctggacg
     4681 ctcttattat ttatgccaat ggcatcttta ttatgattta tctgttatgc atgctggcag
     4741 gctgtaaatt attgcaagga cgttatcgac tactggcggt ggttggcggg ctgttatgcg
     4801 ttctgttact ggcaatggtc ggctggaaaa gtctctatgc gctgatcatg ctggcggggt
     4861 tatggctgtt tctgccaaaa cgaaaaacgc cggaaaatgg cataaccaca taatccggcg
     4921 tttcgacatt aatcctggcg atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt
     4981 cgctggtact caccatcaaa agtattaccg ccaccggtcc cggcgctaaa accgccgcct
     5041 ggcatacgag aaaagcgcaa atgcggcatc aacttcactg tcagatgctt ttgcaccggc
     5101 ggtaataaaa gcagaagacc gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa
     5161 ccggcaatga tcagcgaaac acttttaatc atctccgccg ctgggttttc acccgccgcc
     5221 attttttgct gcatcagcac gaaattctta aagccctggt tacgtaccag tgacatcccg
     5281 ataactgacg tgaatataac cagcacgagg gtcagcaata cccccaatac atgggcaacc
     5341 tgaataaaga ttgaaatctc aatatagaca taaaggaaaa tggcaataaa aggtaaccag
     5401 cgcaaaggtt tctcctgtaa tagcagccgg taaaacccgg ctacctgaat gggttgcgaa
     5461 tcgcgttcag cttatattgt ggtcattagc aaaatttcaa gatgtttgcg caactatttt
     5521 tggtagtaat cccaaagcgg tgatctattt cacaaattaa taattaaggg gtaaaaaccg
     5581 acacttaaag tgatccagat tacggtagaa atcctcaagc agcatatgat ctcgggtatt
     5641 cggtcgatgc aggggataat cgtcggtcga aaaacattcg aaaccacata tattctgtgt
     5701 gtttaaagca aatcattggc agcttgaaaa agaaggttca catgtcaaac aacattcgta
     5761 tcgaagaaga tctgttgggt accagggaag ttccagctga tgcctactat ggtgttcaca
     5821 ctctgagagc gattgaaaac ttctatatca gcaacaacaa aatcagtgat attcctgaat
     5881 ttgttcgcgg tatggtaatg gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa
     5941 ccattcctaa aagtgtagcg aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg
     6001 gaaaatgcat ggatcagttc ccggtagacg tctaccaggg cggcgcaggt acttccgtaa
     6061 acatgaacac caacgaagtg ctggccaata tcggtctgga actgatgggt caccagaaag
     6121 gtgaatatca gtacctgaac ccgaacgacc atgttaacaa atgtcagtcc actaacgacg
     6181 cctacccgac cggtttccgt atcgcagttt actcttccct gattaagctg gtagatgcga
     6241 ttaaccaact gcgtgaaggc tttgaacgta aagctgtcga attccaggac atcctgaaaa
     6301 tgggtcgtac ccagctgcag gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt
     6361 tcagcatcct gctgaaagaa gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg
     6421 aagttaacct tggcgcaaca gcaatcggta ctggtctgaa cacgccgaaa gagtactctc
     6481 cgctggcagt gaaaaaactg gctgaagtca ctggcttccc atgcgtaccg gctgaagacc
     6541 tgatcgaagc gacctctgac tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc
     6601 tggctgtgaa gatgtccaaa atctgtaacg acctgcgctt gctctcttct ggtccacgtg
     6661 ccggcctgaa cgagatcaac ctgccggaac tgcaggcggg ctcttccatc atgccagcta
     6721 aagtaaaccc ggttgttccg gaagtggtta accaggtatg cttcaaagtc atcggtaacg
     6781 acaccactgt taccatggca gcagaagcag gtcagctgca gttgaacgtt atggagccgg
     6841 tcattggcca ggctatgttc gaatccgttc acattctgac caacgcttgc tacaacctgc
     6901 tggaaaaatg cattaacggc atcactgcta acaaagaagt gtgcgaaggt tacgtttaca
     6961 actctatcgg tatcgttact tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg
     7021 tgggtaaaat ctgtgccgaa accggtaaga gtgtacgtga agtcgttctg gaacgcggtc
     7081 tgttgactga agcggaactt gacgatattt tctccgtaca gaatctgatg cacccggctt
     7141 acaaagcaaa acgctatact gatgaaagcg aacagtaatc gtacagggta gtacaaataa
     7201 agaaggcacg tcagatgacg tgcctttttt cttgtgagca gtaacttaaa aataacaacc
     7261 taatatcaac ttgttaaaaa acaaggaagg ctaatatgct agttgtagaa ctcatcatag
     7321 ttttgctggc gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg
     7381 gattgggggt gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg
     7441 tcatttccat tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc
     7501 tggactatct ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga
     7561 tcctcgcacc gatcgtgacc tatttcctga ctatctttgc tggtactggc aacatatctc
     7621 tggcgacact gccagttatc gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc
     7681 cgctgtctac tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg
     7741 cagtggttta catgtcttcc gtgatggaag gtcatggcat cagctacctc catctgctct
     7801 ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat gtcctttctg gtcactatgc
     7861 tgttcaactc caaactctct gacgatccga tttatcgcaa gcgtctggaa gagggcctgg
     7921 ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc
     7981 tgttcctgct gggcgtagtt ggcgtggtta tctatgcaat catcaacagc ccaagcatgg
     8041 gtctggttga aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagcg
     8101 ttgcaactct gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca
     8161 ccttcaaagc aggtatgagc gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata
     8221 ctttcgtttc caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc
     8281 atccgtggct gctggccgtc atcttcttct ttgcttctgc tctgctgtac tctcaggctg
     8341 caaccgcaaa agcactgatg ccgatggctc tggcactgaa cgtttcaccg ctgaccgctg
     8401 ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg
     8461 ctgcggtaca gatggatgac acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc
     8521 cgttcttcat cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg
     8581 gtagcttcat gctgtaatga ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg
     8641 actaacatcc ttcccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct
     8701 tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt
     8761 accgcaccgg atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg
     8821 gcggcctgcg cgaccttgat ccccggcgct acctctctct attactggga aggtaagctg
     8881 gagcaagaat acgaagtgca gatgatttta aaaactaccg tatctcacca gcaggcactg
     8941 ctggaatgcc tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt
     9001 acacacggag acacagatta cctctcatgg ctcaacgcat ctttacgctg atcctgctac
     9061 tttgcagcac ttccgttttt gccggattat tcgacgcgcc gggacgttca cattttgtcc
     9121 ccgcggatca agcctttgct tttgattttc agcaaaacca acatgacctg aatctgacct
     9181 ggcagatcaa agacggttac tacctctacc gtaaacagat ccgcattacg ccggaacacg
     9241 cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca tgaagatgag ttttacggca
     9301 aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg
     9361 gagcgacgtt aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag
     9421 aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt
     9481 ctgttccgca gcaagagcag cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt
     9541 tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg
     9601 gcatcgtgct gggtggtaaa cagcggctct ccactgccag agcattgttg ctgaccttta
     9661 tttatgtgca ggggatggca ctgacttaca cggcgctggg tctggtagtt gccgccgcag
     9721 ggttacagtt ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct
     9781 ttaccttgct ggcgatgtca atgtttggct tgtttactct acaactcccc tcttcgctgc
     9841 aaacacgtct cacgttgatg agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg
     9901 ttatgggggc gattgccgga ctgatctgtt caccatgcac caccgcaccg cttagcgcga
     9961 ttctgctgta tatcgcccaa agcgggaaca tgtggctggg cggcggcacg ctttatctct
    10021 atgcgttggg catgggcctg ccgctgatgc taattaccgt ctttggtaac cgcttgctgc
    10081 cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac
    10141 tgccggtctt cctgctggag cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg
    10201 cgctgggtgt cgcattcttt ggctgggcct ttatcaccag cctacaggct aaacgcggct
    10261 ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt ggttagcgtg cgcccacttc
    10321 aggattgggc atttggtgcg acgcataccg cgcaaactca gacgcatctc aactttacac
    10381 aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccggtga
    10441 tgttagatct ttatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca
    10501 gcgacccgca ggtgcaaaaa gcgttagcag acacggtctt acttcaggcc aacgtcacgg
    10561 ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa
    10621 ttctcttttt tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg
    10681 atgctgaaac cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg
    10741 gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat
    10801 tagaattaca agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc
    10861 cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc
    10921 gttatcttag ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa
    10981 ccgccgaaca aaagctgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc
    11041 gctatccggg ctgtctattt atcgctgcct gtacgtttta tcccgatcct ggccacccta
    11101 ttcatcaact ggccgatcag caaaaaagcg cggcctacga tttcacccac gaactgttaa
    11161 ccacactgga agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag
    11221 gctgtttaag ccgaatgctg gtgaatcgta gccaggcaga tgtcgacact gcacatcggc
    11281 tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg tggtgcattg acctgacaga
    11341 aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt
    11401 aggttgacga gatgtgcaga ttacggttta atgcgccccg ttgcccggat agctcagtcg
    11461 gtagagcagg ggattgaaaa tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa
    11521 ttcatatcaa cggacctcca cggaggtccg tttttcgttc caggacgcca cgatttaagc
    11581 gtcctgcctc caaatcaatt ctaccgaact caaccagatt ccccccacat caacctcatt
    11641 gtgtgggtat aattgtgggt ataccccagt tcgacagaat ttgtaccctc tttaacgcat
    11701 tcagaaggct gaactatggc actgactgac gcaaaaatcc gggcagcaaa gcctactgac
    11761 aaggcttata aactcactga cggggctagc atgttcctgc tggtacatcc taatggttcc
    11821 cgttactggc gtctccgtta tcgtattctg ggtaaggaga aaactctggc acttggtgtg
    11881 tatccagaag tttctctttc tgaagctcgt acaaaacggg ataaggcccg aaaactgatt
    11941 tcggagggga ttgacccttg cgaacagaaa agaactaaaa aagtagtccc tgatttacaa
    12001 ctatcttttg aacatattgc acgacgctgg catgccagta ataaacaatg ggcacaatca
    12061 cacagcgata aagtactcaa aagcctcgag acacacgttt tcccctttat cggcaaccga
    12121 gatatcacaa cactcagtac cccggacctg cttatccctg ttcgtgctgc agaagctaaa
    12181 caaatttatg aaatcgccag tcgtctgcag caaagaatat ctgctgtaat gcgttatgcc
    12241 gtacagtctg gcatcatcag atataacccg gccctggata tggctggtgc attgaccacg
    12301 gtaaaacgca agcatcgtcc tgcactggat ctttcacgcc tgcctgaact tctgtcgcgt
    12361 attgacagtt ataaaggcca gcctgtcacc cggctggcag ttatgctgaa tttactggtt
    12421 tttattcgct ccagtgaact cagatatgcc cgctggtctg aaattgatat tgacaatgcc
    12481 atgtggacta ttccagccga acgcgaacct ctgccaggcg taaaattctc acaccgaggc
    12541 tccaaaatgc gaaccccaca tcttgtgcca ctcagcaaac aggctgtagc catactaaca
    12601 gaacttcaga catgggctgg tgaaaatggt ctgatcttta cgggagcgca tgacccacgt
    12661 agaccaatca gtgaaaacac cgtaaataaa gccctgaggg ttatgggata tgacacaacc
    12721 caagaagtct gtggtcatgg attccgggcg atggcatgta gtgcgctgat cgaatcaggt
    12781 ttgtggtccc gcgatgctgt tgaacgtcag atgagccatc aggaacgtaa cggtgtacgt
    12841 gctgcttaca ttcataaagc agaacatctg gaagaacgcc gattgatgct acaatggtgg
    12901 gccgattttc tggatgcaaa cagagaaaaa tttatcagtc catttgaata tgcaaaaatt
    12961 aacaatccat taaaatagta atcatcccgg ggaaatgccc gggaattatt ttcaatatct
    13021 ttttctttga gagtaatgaa taagccagac aatggacaga accattttct ttacgtcatg
    13081 taatcaaact gtttatagcc gatatcaggg atatctgatt ctcccagtag cacgtaatac
    13141 ctcgtcaggt attttcgtgt gttggtaata tatccaatta gtagaacatg aatttttcga
    13201 tgaaagcacc aataaccttt tcatataact caacagagcg tcagaaacag atagcccggc
    13261 ggccagacgc ttgatgctgg ttctcagtgt caggtgttcc gctcaatacg ctgagctaat
    13321 cttttaccac tcagatgctt ctcgttcgga atttctctgg cataacagtc ccagtcatca
    13381 ctggttatca taccgatatt aagcgatgtg agcagagcta agcaactcac ggcaggtgtc
    13441 atctataaag ggccaaaggt atacaccaga acgccaccta ttttggtgtt gtaggcatac
    13501 caaaaccaat gttgccagac tttactgccg acaaaactcc attgctcatc gaattcgcag
    13561 atgagtgcca catcggcatt agcaaccgga gatgatgtta ttcgcttcgg cgcaagtttt
    13621 ttaaagtgcg aatgacggtg ttaataccgt ttttaagcgt ccttgccgta ttccggacac
    13681 cggttccatt gaatacaatt ttggtatttt tttgacactg ggcttacggg attcataggt
    13741 ataaatgagt ataaacacgc gatggcagtc tcgaaagcgc tcacggcctt tagagctcac
    13801 ctcatggcgg taaatctgag cagactgaca acggggacaa tgaacgataa cgttgaccat
    13861 aagaaaacct caaaagtggg tatta
//
LOCUS       DAZFAH010000115        13878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFAH010000115 DAZFAH010000000
VERSION     DAZFAH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.115"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(172..1059)
                     /gene="rarD"
                     /locus_tag="ACHJWB_003170"
     CDS             complement(172..1059)
                     /gene="rarD"
                     /locus_tag="ACHJWB_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4314751.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1111..1578)
                     /gene="yigI"
                     /locus_tag="ACHJWB_003171"
     CDS             complement(1111..1578)
                     /gene="yigI"
                     /locus_tag="ACHJWB_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4314752.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1743..2612
                     /gene="pldA"
                     /locus_tag="ACHJWB_003172"
     CDS             1743..2612
                     /gene="pldA"
                     /locus_tag="ACHJWB_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4314753.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2745..4574
                     /gene="recQ"
                     /locus_tag="ACHJWB_003173"
     CDS             2745..4574
                     /gene="recQ"
                     /locus_tag="ACHJWB_003173"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4314754.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTVCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAACLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4638..5258
                     /gene="rhtC"
                     /locus_tag="ACHJWB_003174"
     CDS             4638..5258
                     /gene="rhtC"
                     /locus_tag="ACHJWB_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4314755.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHJWB_003175"
     CDS             complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHJWB_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4314756.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6051..7073
                     /gene="pldB"
                     /locus_tag="ACHJWB_003176"
     CDS             6051..7073
                     /gene="pldB"
                     /locus_tag="ACHJWB_003176"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4314757.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7081..7881
                     /gene="yigL"
                     /locus_tag="ACHJWB_003177"
     CDS             7081..7881
                     /gene="yigL"
                     /locus_tag="ACHJWB_003177"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4314758.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7957..8856
                     /gene="bioP"
                     /locus_tag="ACHJWB_003178"
     CDS             7957..8856
                     /gene="bioP"
                     /locus_tag="ACHJWB_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4314759.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHJWB_003179"
     CDS             complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHJWB_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4314760.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9933..12194
                     /gene="metE"
                     /locus_tag="ACHJWB_003180"
     CDS             9933..12194
                     /gene="metE"
                     /locus_tag="ACHJWB_003180"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4314761.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALVLGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12316..12438)
                     /locus_tag="ACHJWB_003181"
     CDS             complement(12316..12438)
                     /locus_tag="ACHJWB_003181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314762.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12479..12724)
                     /locus_tag="ACHJWB_003182"
     CDS             complement(12479..12724)
                     /locus_tag="ACHJWB_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4314763.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12714..13199)
                     /locus_tag="ACHJWB_003183"
     CDS             complement(12714..13199)
                     /locus_tag="ACHJWB_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HFO4314764.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPDLAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTHTATLEDYKLKGELNDLYHQDALDYD
                     N"
     gene            complement(13179..13840)
                     /gene="tssD"
                     /locus_tag="ACHJWB_003184"
                     /pseudo
     CDS             complement(13179..13840)
                     /gene="tssD"
                     /locus_tag="ACHJWB_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
BASE COUNT         3315 a         3465 c         3636 g         3462 t
ORIGIN      
        1 tttgtcaaat ccttggtaga tatacgatgt ctctaggatg gagtaagtac tggggagttt
       61 tgggggtatt tcctgtaaca aatattgttc tttttcttat cttaattttt ttcaaaaaaa
      121 gataaataag tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt
      181 tctacgctgc gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc
      241 gaaagtcacc atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa
      301 catcagcgtc gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc
      361 agcggcggta aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa
      421 attcagcgac atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag
      481 gtaaattgcc gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc
      541 aacggcaatc ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc
      601 gataataggt agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat
      661 caccgccagc cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat
      721 gttcaccagc gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac
      781 cgcccagata aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa
      841 aattttctgt ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat
      901 cagcaccacc ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcggg
      961 cacgtagtaa atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag
     1021 agcgagtaat acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt
     1081 ggtaacagca gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact
     1141 ggcaatataa agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc
     1201 tgcacgcaac aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc
     1261 aacgcgaaga tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat
     1321 ggtttcgtgg cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc
     1381 cgacgcaatg accccgccgt gcaaaatgct ttgcgcccag ttgcccacca tcattggctg
     1441 atttttaaag gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc
     1501 gcggttaaat ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc
     1561 ggtcagtacg gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat
     1621 gcttctttgt tgttggtttc tactttagga agggataact aacggctatg gagttaagta
     1681 tgtaaaatag cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac
     1741 cgatgcggac tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg
     1801 cacaagaggc aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg
     1861 ccaatatgct gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc
     1921 tcatttacac ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg
     1981 aaaatgcgcg taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg
     2041 ggattttagg cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac
     2101 tgtccaatag cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc
     2161 tcggttttgc caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt
     2221 ataaccacga ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata
     2281 ctcgcctgat ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg
     2341 gtaatactga cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg
     2401 gctatcacct cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct
     2461 acggcggcgc ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc
     2521 aggtttacag cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg
     2581 gtgtgggggt tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg
     2641 aacccagcat ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc
     2701 gctgaaaata gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag
     2761 tgttgaatct ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt
     2821 ttcgccccgg ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg
     2881 tcatgcccac cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg
     2941 gccttaccgt ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc
     3001 aagccaacgg cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg
     3061 aagtgatgac agtctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc
     3121 tgatgctgga taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg
     3181 atgaagcgca ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg
     3241 gtcagttgcg ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg
     3301 acaccacgcg ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca
     3361 gcagttttga ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc
     3421 agttgatgcg ctacgtgcag gaacagcgcg gtaagtcagg cattatctac tgcaacagcc
     3481 gcgcgaaagt agaagacacc gctgcgtgcc tgcaaagcaa gggaattagc gcggcggcct
     3541 atcatgccgg gctggaaaat aatgttcgcg ccgatgtgca ggaaaaattc cagcgcgatg
     3601 acctgcaaat tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc
     3661 gcttcgtggt ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggac
     3721 gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gctgatatgg
     3781 cgtggctgcg ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc
     3841 ataaactcaa tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc
     3901 tgaactactt tggcgaaggg cggcaggagc cgtgcggaaa ctgcgatatc tgcctcgatc
     3961 cgccgaaaca gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg
     4021 tgaatcagcg gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc
     4081 gtatccgcga ctatggtcat gacaaactga aagtctatgg catgggccgt gataaaagcc
     4141 atgaacattg ggtgagcgtg atccgccagc tgattcacct cggcctggtg acgcaaaata
     4201 ttgcacagca ttctgccctc caactgacag aggctgcgcg tccggtgctg cgcggcgaat
     4261 cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat
     4321 cgttcggcgg caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga
     4381 ttgccgatga aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga
     4441 tggctgaaca gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc
     4501 gcaagctgga acgctttggc aaaccgttta tggcgctgat tcgtgcgcat gttgatggcg
     4561 atgacgaaga gtagtcagca gcataaaaaa gtgccaggat gaagactccg taaacgtttc
     4621 ccccgcgagt caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg
     4681 cgcttatgag ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc
     4741 gtaaagaagc gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga
     4801 ttgcgttgct tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta
     4861 tggtgggcgg tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga
     4921 aaaaagaggc tgtatctgcg cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt
     4981 tcctgaaagg cttactgacc aatctcgcca atccgaaagc gattatctat tttggttcgg
     5041 tattctcatt gtttgtcggc gataacgttg gcactaccga gcgctggggc attttcgcgt
     5101 tgattattgt cgaaacactg gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc
     5161 aaatgcgccg tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat
     5221 ttgccggatt tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta
     5281 agtcggataa ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc
     5341 taacagtgct cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg
     5401 ctttggtcct ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac
     5461 aataatatcg accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg
     5521 ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt
     5581 gagattcaca aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga
     5641 tttaaggtca attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc
     5701 cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa
     5761 ccccacgcca accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc
     5821 caccgcgccg cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc
     5881 tggcgacagg cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat
     5941 gatgaactcc cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc
     6001 aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc
     6061 agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga
     6121 ctgatttctg gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc
     6181 gctttgtccg ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta
     6241 ttgagagcta cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg
     6301 tcttaatcat cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc
     6361 tcgggcatgt taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg
     6421 aggttcagcc cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga
     6481 tctccacatt atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc
     6541 caatgtttgg gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg
     6601 ccgaagcgca tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt
     6661 tgccgtttgc tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct
     6721 tctatgctga tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa
     6781 gtattctggc tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc
     6841 tcttacaagc tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac
     6901 tccgcaccgc cgcgggccat cctgtcgaag gaggacgacc gttggtaatt aaaggtgctt
     6961 accatgagat cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccattgttg
     7021 attttttcaa caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt
     7081 atgtaccagg ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta
     7141 tccccttacg ccaaagaaac cctgaagctt ctcaccgcgc gcggcatcaa ctttgtgttt
     7201 gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gatcaagtcg
     7261 tacatgatca cctccaacgg cgcgcgcgtt cacgatctgg atggcaatct gatttttgct
     7321 cataacttgg atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac
     7381 atcattacta acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag
     7441 atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag
     7501 ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc atgaacaact gctgccgctg
     7561 gagcaggcga ttaacgctcg ttggggcgat cgcgtcaacg ttagtttctc taccttaacc
     7621 tgtctggaag tgatggcggg cggcgtttca aaaggccatg cgctggaagc ggtggcgaag
     7681 aaactgggct acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgatgccgaa
     7741 atgctgtcga tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac
     7801 cttcatcccg agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg
     7861 cgtaaactct atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc
     7921 tacaatggat actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg
     7981 attctgtggg ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc
     8041 tattttgcgg tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt
     8101 acccgtggca atagcctgaa aacagtcggc ctgtatatgc tggtgggcgc gatgcagctc
     8161 ggcgtgatgt atatgctgag tttccgcgct tatctctacc tgacggtttc cgaactgctg
     8221 ctgttcaccg tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc
     8281 cgtctgcgct ggggatatgc ttttagtgcc ttgttggcgg tgattggtgc cgggattatt
     8341 cgctatgatc aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat
     8401 atcacttttg ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg
     8461 cagcataacg cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg
     8521 ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg
     8581 tttcttggcg tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag
     8641 gtggacgccg gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggtt
     8701 aacctggcta tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg
     8761 atcctggcct cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca
     8821 gatgatcgca ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct
     8881 gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg
     8941 tcaccaccag accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac
     9001 ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg
     9061 ggctgacgcc tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg
     9121 gataaattaa tagcgtctcg ctggcgagat cttccggtgt aatacgcgtt tttgccgcca
     9181 gtggatggtc aggtgctaac accaggcgta cttcataatc gaacatcggc gaataatgca
     9241 ggccactgcg cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg
     9301 cgggctgcgg gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct
     9361 tatggaaatt ttctaacgcg ggtgtcagcc actgaataca gctatggcac tcaatggcaa
     9421 tgcgcagacg cgtctgctgc ggttcattgc aggcttgtag tgcctggctg atttgcggca
     9481 gcacctggtt tgccagttgc aacaggattt ctccctgcgg tgtaaagcga agcggctggc
     9541 ttttacgcac aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca
     9601 gggcggattg cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca
     9661 acgcttgtag cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga
     9721 ataatttgcg cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac
     9781 ggctaaaatc cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc
     9841 acttacttca gtaagctccc ggggatgaat aaacttgccg ccttccctaa attcaaaatc
     9901 cataggattt acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt
     9961 tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca agagagttat tgggcaggga
    10021 actccacgcg tgaagaactg ctggcggtag ggcgtgaatt gcgtgctcgt cactgggatc
    10081 aacaaaagca agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg
    10141 tactgaccac cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt
    10201 cggtagatat cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg
    10261 cggcggcagc ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt
    10321 tcgttaaagg ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg
    10381 cgctggtgct gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc
    10441 tggggaaagt gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg
    10501 tttatcagca agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg
    10561 aacccgcgct ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg
    10621 acgcgctcca gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa
    10681 atctcgacac gattactgcg ctgcctgttc agggtctgca tgttgacctc gtacatggta
    10741 aagatgacgt tgctgaactg cacaagcgcc tgccttctga ctggttgctg tctgcgggtc
    10801 tgatcaatgg tcgtaacgtc tggcgcgccg atcttaccga gaaatatgcg caaattaagg
    10861 acattgtcgg caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca
    10921 tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc
    10981 tacaaaaatg ccatgaactg gcactgctgc gcgatgcgct gaacagtggt gacacggcag
    11041 ctctggcaga gtggagcgcc ccgattcagg cgcgtcgtca ctctactcgt gtacataatc
    11101 cggcagtaga aaagcgtctg gcggcgatca ccgctcagga cagtcagcgt gcgaatgtct
    11161 atgaagtgcg tgctgaagcc cagcgtgcgc gttttaaact gccagcatgg ccgaccacca
    11221 cgattggttc tttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg
    11281 gcaatctcga cgccaacaac taccgcacgg gcattgcgga acatatcaag caggccattg
    11341 ttgagcagga acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca
    11401 tggtggaata ctttggcgag cacctcgacg gatttgtctt tacgcaaaac ggttgggtac
    11461 agagctacgg ttcccgctgc gtgaagccac cgattgtcat tggtgacatt agccgcccgg
    11521 caccgattac cgtggagtgg gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag
    11581 ggatgctgac ggggccggtg accatactct gctggtcgtt cccgcgtgaa gatgtcagcc
    11641 gtgaaaccat cgccaaacag attgcgctgg cgctgcgtga tgaggtggcc gatctggaag
    11701 cggcagggat tggtatcatc cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc
    11761 gtcgtagcga ctgggacgcc tatctccagt ggggcgtaga ggccttccgt atcaacgccg
    11821 ccgtggcgaa agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca
    11881 tcatggattc cattgcggcg ctggatgcgg atgtgattac catcgaaact tcgcgttctg
    11941 acatggagtt gctggagtcg ttcgaagagt ttgattatcc gaacgaaatc ggtccaggcg
    12001 tctatgacat tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga
    12061 aagcggcaaa acgcattccg gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa
    12121 cgcgcggctg gccagaaacc cgcgcagcac tggcgaatat ggtgcaggcg gcgcagaatt
    12181 tgcgtcgggg atgatgaata ttaacgagtc actgtaaatt gcagtacgat gaaagaggca
    12241 aaactgcctg ataccaggcc tacataattt atgcaattta ttgaattttt gggttttgtt
    12301 ggccggacaa ggcgttcacg ccgcatccgg catgaacaac gcgcactttg ccaataatct
    12361 gatggcttcc atcaccggaa gccattttgc tatctgcgcc cgttcagata cagaaataac
    12421 cgaatcattt ttacgcaaaa tataatcctg agcgatttgt tcagtaatca acgctacgtt
    12481 atttattctc acttttcacg aaattgtatc ccttttcagt aacgtggtag tgaggcatag
    12541 acttatgtcc gctatcctca ataataacca aaccctcgtt agctaatttg ttaacaatgt
    12601 taacaacatt accgaatcct ggaatttgcc ttatggatag tgtgcgatcg agattcatcc
    12661 agttccagtt tatatctttc tcagccataa ctttcagtac gttaatttca gtttcagtta
    12721 tcatagtcaa gtgcatcctg gtggtataag tcattaagtt ctcccttaag tttataatct
    12781 tctagagtag cagtatgcgt atgattccag agtgcatagc ctgcgtcacc atcaggttgt
    12841 cctgtttccc aaccaagatt acagtaacgc tgatattccc gacactcatg agtatagaag
    12901 ttaagatcta cttgttctgg ggggattttt cctttctcaa tgtctttcag tctattgatc
    12961 atcacttcat ttacagggtc atgaccaaat ctggctatgt gtttttcaac aatagcgata
    13021 ccttccctgt tgattgttac gccatctgtc gtcagatttt ctattggacc accggcaagg
    13081 tcgggattaa atggtcttcc tgatactgct cctctggctc cgaaatgctc aagtgattcc
    13141 aaatcaggga gtctcattct ccccagaact gctccagcaa ctattgaagc accagcgatg
    13201 agtaaccctt tgttatccat aaattgatcg taaccagcag gtgcatctcc ccccaacttg
    13261 tctgctgttt ccctgaatcc ttcgatattc ccattgtata tgccgacagc agcaagcagt
    13321 ctgccaaccg ctttactatt aagtgttttg aaaccattac cagctttagt ttttggccgt
    13381 ggtataaaca gacggttgta gttatcggga aatgcccaat aactaaattc agtattagca
    13441 atcagatgcc tggagagtat atattaataa ctgacagtga tggcttctgt atccagattg
    13501 ttctcagtaa acaggtgatt gattgctgat aagagcgcgc cgcggagttg aatgttgtaa
    13561 tatttctccc atcgaccaaa cctattgatc cgatagaagt caaactccat gaacaactgc
    13621 tcgttattgt taatagcgtt aataagaatt tacagaaact aaggccatgc aattgtgacc
    13681 ctttgcctgc attcgtaata ctattagaga gcgagaacgc gaagatttca tcttcatggc
    13741 caatctgcca gcgattacct attgatgcgc tcgttccaca aagagaggaa atacatcctt
    13801 gttgttctcc ggttatggtc aagtaaataa tatcactcat ccttaaaaaa ccttttgtta
    13861 attcttttgt gaaagaga
//
LOCUS       DAZFAH010000116        13783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFAH010000116 DAZFAH010000000
VERSION     DAZFAH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.116"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..582
                     /locus_tag="ACHJWB_003185"
     CDS             16..582
                     /locus_tag="ACHJWB_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4314765.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /locus_tag="ACHJWB_003186"
     CDS             633..3092
                     /locus_tag="ACHJWB_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4314766.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGIL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /locus_tag="ACHJWB_003187"
     CDS             3107..3838
                     /locus_tag="ACHJWB_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4314767.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDYAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3835..4896
                     /locus_tag="ACHJWB_003188"
     CDS             3835..4896
                     /locus_tag="ACHJWB_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4314768.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /locus_tag="ACHJWB_003189"
     CDS             5048..6094
                     /locus_tag="ACHJWB_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4314769.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLEIAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHJWB_003190"
                     /pseudo
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHJWB_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHJWB_003191"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHJWB_003191"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4314770.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKRIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHJWB_003192"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHJWB_003192"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4314771.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGSIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHJWB_003193"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHJWB_003193"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4314772.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHJWB_003194"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHJWB_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4314773.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRTLSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHJWB_003195"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHJWB_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4314774.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHJWB_003196"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHJWB_003196"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4314775.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /locus_tag="ACHJWB_003197"
     CDS             complement(13199..13708)
                     /locus_tag="ACHJWB_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4314776.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3313 a         3703 c         3399 g         3368 t
ORIGIN      
        1 ttaaaggact ctgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcgatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtatcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggacggcagc agcagcacta acctgaacgc ctacggtacc accggaatta
     1201 acgccggggc atggcgctta cgtagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgga atatcgcgca cctatctttt tcgtccatta ccgcaattag
     1321 gctctaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgagc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc taccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgact atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac tgcccaaaac
     4441 ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggagatag cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 actaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcag gtatggcgtg
     5881 gggactgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagtcccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa
     6121 cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc
     6181 atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttataaaaac ggatgcgtgg tcagttgttc
     6541 cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta agcggggtta tgctgcgaca acaatcctct ttttggcaaa tgtagctcgt
     6961 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
     7021 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
     7081 gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcagctgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aatcgcgaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc tgcatgaaat
     7561 ccacaagcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta attgttcgaa
     7681 ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatag gctgcccgtt
     7861 atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg ggctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 actacccgcg accgccagat aactgcgcat gccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca agcgccagcc ggtccagacg gcgttatcat ccagccgcgc
     8341 ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa ccgttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca ggttagcaat
     8461 gcgtaacgca ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagcaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgtgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgcaggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcacccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca gccgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tattgatggc gatcggtcat gtggtgttgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgataatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcggcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggttgg catgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcactc atacccgcgg cgttaacaaa
    10861 aaagtactgc gtgcgacaaa ctttctcctg ccgaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcactgat taccgtgctg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc ggaaggagct gggactgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggtagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgacc gcgatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    11341 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    11401 gtgttaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt agctatgtcg
    11461 cagattacgc gcattgaaat ccccggcgtg accggcgtat taaccggcat ttatatgctg
    11521 ctttccggcg cgattgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctggcg ctggaatctt aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg ggttgtcggg
    12001 ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca ctgccagcca
    12061 ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg tagccggtta
    12181 tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg gtatcccgtt
    12241 ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac acgatccgtc
    12301 caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct
    12661 atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc
    12781 atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtagtc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc agccagctgc tggcgttctg
    13381 tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacattg gtgtgatcgc
    13441 gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg gcggtactgg ttgtaatagt
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcaaagata cggattaggg
    13681 tgctgtcatc aaatcgttgt agattcatta ctgctcctga acggtataaa ggagtaagtg
    13741 tagacgatgg taaatgaatt ttatggggca gagtttttgc ggg
//
LOCUS       DAZFAH010000117        13653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFAH010000117 DAZFAH010000000
VERSION     DAZFAH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.117"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..1109)
                     /gene="mhpE"
                     /locus_tag="ACHJWB_003198"
     CDS             complement(96..1109)
                     /gene="mhpE"
                     /locus_tag="ACHJWB_003198"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4314777.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(1106..2056)
                     /gene="mhpF"
                     /locus_tag="ACHJWB_003199"
     CDS             complement(1106..2056)
                     /gene="mhpF"
                     /locus_tag="ACHJWB_003199"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4314778.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEI
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(2053..2862)
                     /gene="mhpD"
                     /locus_tag="ACHJWB_003200"
     CDS             complement(2053..2862)
                     /gene="mhpD"
                     /locus_tag="ACHJWB_003200"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4314779.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(2872..3738)
                     /gene="mhpC"
                     /locus_tag="ACHJWB_003201"
     CDS             complement(2872..3738)
                     /gene="mhpC"
                     /locus_tag="ACHJWB_003201"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4314780.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(3756..4700)
                     /gene="mhpB"
                     /locus_tag="ACHJWB_003202"
     CDS             complement(3756..4700)
                     /gene="mhpB"
                     /locus_tag="ACHJWB_003202"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4314781.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(4702..6366)
                     /gene="mhpA"
                     /locus_tag="ACHJWB_003203"
     CDS             complement(4702..6366)
                     /gene="mhpA"
                     /locus_tag="ACHJWB_003203"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4314782.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            6557..7390
                     /gene="mhpR"
                     /locus_tag="ACHJWB_003204"
     CDS             6557..7390
                     /gene="mhpR"
                     /locus_tag="ACHJWB_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4314783.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7467..8549
                     /gene="lacI"
                     /locus_tag="ACHJWB_003205"
     CDS             7467..8549
                     /gene="lacI"
                     /locus_tag="ACHJWB_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4314784.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            8672..11746
                     /gene="lacZ"
                     /locus_tag="ACHJWB_003206"
     CDS             8672..11746
                     /gene="lacZ"
                     /locus_tag="ACHJWB_003206"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4314785.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENSTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPERAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWLHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            11798..13051
                     /gene="lacY"
                     /locus_tag="ACHJWB_003207"
     CDS             11798..13051
                     /gene="lacY"
                     /locus_tag="ACHJWB_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4314786.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            13117..>13653
                     /gene="lacA"
                     /locus_tag="ACHJWB_003208"
     CDS             13117..>13653
                     /gene="lacA"
                     /locus_tag="ACHJWB_003208"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO4314787.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPC"
BASE COUNT         3088 a         3796 c         3617 g         3152 t
ORIGIN      
        1 tctcacgccc cccgtaggcc ggataaggtg tttacgccgc atccggcagt cgtgctggga
       61 tgggcataca aaatgcacgt tctcggcagt catcattatt tgttgttgcg cagatccagc
      121 gccacgtcaa cgatcatatc ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg
      181 agaatatcca ccgcacttaa gccataacgc gccgccgccg tttcacagtg acgcaggaag
      241 ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc gatcgactcg taccggtcga
      301 tcctgcaacg gacgcaccag gtcgtcggcg gcatccatta acgcatagag atcggtccca
      361 tgctgccagc ccagtttatc cgcggcggca ataaacactt ccagcggtgc gttacctgcg
      421 cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc agccctcttc caccgccgcg
      481 atagagttcg ccacgccaag actcaggtta tggtgagcgt gcatgccagt ttgcgtttct
      541 ggtttcagct ctgctttcag ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca
      601 ccgccagaat ccaccacata aatacaggtc gcaccgtagc cttccatcag ctttgcctgc
      661 tttgcgagat tctccggcgt ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt
      721 ccgagctcgc gggcatactg aatatgctgg gcggaaacat cagcttcggt acagtgcgtt
      781 gccacacgaa ccacccgcgc gccagcctgc caggcatttt tcagatcgtg aatagtgccg
      841 attcctggca gcaacaacgt cgcgattttg gcgtgcttca ccacatccgc cgctgcttca
      901 atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa agctggaacc ctgcaaaccg
      961 tcgccatggg ccacttcaat cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg
     1021 cgaacgtttt ccagcgaata ctgatgacga atggcgtgca taccgtcacg caacgtgacg
     1081 tccgagatat aaagtttttt accgttcatg ccgcttctcc tgccttgcgc gccagtgact
     1141 gggccatttt ttccgctgtc gccagcgcac tggaagtcat aatgtcgagg ttgcccgcat
     1201 aggcaggcag ataatgcgct gcgccttcga tttccagcca gaccgctgtt ttcagtccgg
     1261 agaattgccc cacgcccggt aaattgaccg gtttatcctg cgggataact tcaaactgca
     1321 cgcgctgttt caggcgataa cccggtacgt aagcctgcac cgcctcagcc atttcattga
     1381 ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca
     1441 tcatcaacgg tggctctgct gggttaagca caataatcgc cttccctttt gccgcgccgc
     1501 ccaccacttc aatggctcgg gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc
     1561 caggtccggc agatttactg gcgatagaag cgataatttc ggcgtaatga acacgcgcca
     1621 cgcgtgaaac tgccgccacc attggaatgg tggcctggcc gccgcaggtg accatgttga
     1681 cgttcagttg atcgacgttc gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg
     1741 cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt
     1801 ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat gtcgatatca gcaaattcag
     1861 gcatgttcat cagcccgatc accccttcat gggtggtggc gacgcccata cgtctggcac
     1921 gcgccagacc gtcggactga ggatcaatgc cgaccatcac cgccatctcc agatgctgac
     1981 cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt gccagaaccg ataatggcga
     2041 ctttacgctt actcatgaca gacttccttt tggggctgcg cttgaaaatg tcgcagcaac
     2101 tgaacctatg ccttcaatat gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg
     2161 gcctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc ggttcaccca gactggccat
     2221 tttgcgtgcc agccagacgg cggcattaag cggatgtccc aggcattcgc tgccgcgccc
     2281 gctggaaacc tcttcgttat tacgcgtcat cttcatggcg cagtttttca ggtctaaccc
     2341 cgccggacgt tgcgccggac cgccgatgac atacaccccg caggaagcgt tatctgccac
     2401 ggtatcgaca aactgaatcg accagtcgcg aatgcggctc cccaccactt ccagcgccgg
     2461 aagtacccat tcaatggcgt tatacagttc gtcgaaggtg atatcagttg cgggtaaatc
     2521 gcggttcaac accagtgcga tctccgcttc aatgcggggt tgcagaacac gggaaaaagg
     2581 aatgatttcg ttatcgccat aacacatgtc ggcaaataac gtcccaaaat ccggttgatc
     2641 aacgcccagt tgttgttgca ctttcggatg cgtcaggccc actttacgcc ctaccacgcg
     2701 acgcccctgc acaacgtcat attgcacatt tatgtgctga atggcgtagg ccgcttcagc
     2761 gttatcgata ccaatcagat cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg
     2821 gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc atgaccattc cttaagggcg
     2881 tgcgaggaaa ttcagcacca attgattgaa agcgtcggca tgttcccact gcgcccagtg
     2941 accacagtcg cggaagatat gcagttcaga accggcaatg ccggacagca gacgcagacc
     3001 cgcatccatc ggcacaaagc ggtcgttgcg cccccagaca atcagggttt gcgctttgat
     3061 ttccgccaga cgtgggccaa aatccgggaa ctgtttcgga ttagcttcca ggctcttaac
     3121 gaagttttcc aggtgatcgc ggcgcgacag catattattc aggcgcgctt caaacagggc
     3181 gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc atcagcttca ggttttcgat
     3241 agtcggctga cgataaagct gattcagtcg cttaatacct tcggttggca tcggcgtaaa
     3301 caaactcatg ccgcccgtac cgccgcccat cagcaccagt ttgccgacgc gctccggcca
     3361 gttaagggtg aacgccacag aactatggcc gcccatcgag ttgcccagca ggtggatttt
     3421 ggcgatatcc agttgatcca ccacgctttt caggattcgt gcattaagat ccgatcgtga
     3481 accactatta acgatcgaat cgcttttgcc ccaacccgga caatccagca ggattacccg
     3541 atagcccgcc tctaccagcg gatcgatatt gcggctgaag ttcgcccagc cagtagcacc
     3601 cgggccggag ccatgcagca ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg
     3661 gatgcgcagc gttttacccg cttcttctac attcagaaaa cggctggtgg cggcttcggt
     3721 ttgtggctga taactcatca tcttctcctg catattcagt tctctgttct ggcgcttaac
     3781 gagccaaatc cggcaatcca ctccgggatt gggcggtaat aacgcccttc gctacgccag
     3841 ttgccaaatg tagaaatagc ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac
     3901 tttccggcaa tggcggaaag ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc
     3961 tgctccagca aagtcatgaa ctggttatcc caaatcgggt tgagcggatg cagagttctc
     4021 tgatcctcaa caaacttctc agcggcgcta atcacccgtt gctgacgcaa ttcgcgctca
     4081 ctggcgggta aatctttccc gctccccaac agacggtcgc gcatatgggc atcggctttc
     4141 gccagttcgg gcaccggcgg ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt
     4201 ttattgagag tgctggtgaa acgtccaatg gcttcaccca acatgcgggt acgctggaaa
     4261 ccgggcagcg gcgtggcgac accgttgatg aacacaggca gaactggcac cttatccagc
     4321 ccaccgagca ggaactccag cggctgggcg aacccgtggt ccacctgcat acagtaagaa
     4381 acggcaagat cgatcccgct cttcatgaca gcatgcgcac aggcctccgc cagctccaca
     4441 ggcacgggca gctctcctgc cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct
     4501 aaacagaacg gtggcatcac gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc
     4561 accaccagtt caggggagaa agccgcaata cgctcgcggg cgctggcaat cacgccattg
     4621 acctcatcga gtacctcttg cgccgggtcg acatacccca ccaacggcga gtgggaaaga
     4681 cagtgaagat aagcgtgcat atcaggctac cttttcgaca gaaacgtcgg catcagggcg
     4741 ggtcagcgtc atcaccgacg ccagtttatt cagcgtattg cccagggttt gcggaatggc
     4801 ggtggcggca acaaagcgat ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa
     4861 ccagctacgc agacgacctt gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg
     4921 tgcggtatga atttgcactt ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca
     4981 ctgctggatt tgctcgtcgc tcatccccca cagtggattg catccccagc caattaccgc
     5041 gaagttcgcg ccaatcgcgt tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt
     5101 cggctgaata aacatcttgc cgaccggaga gtgcttcgct tcgccctcac gtaccagcgc
     5161 accgccgtaa tattgcggca tcggcttgaa gcgcatttcg aggaagtagc gttttactgg
     5221 cggcagataa ttcagtagcc aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg
     5281 cggagccagc acgttgcccg ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg
     5341 acgttcttgt tgataggtat cgagcagcgc atcgcgggct ttcccctgga taaccaacgc
     5401 cagtttccat gcgaggttaa aggcgtcgcg cataccactg ttatagccct gcccctgcca
     5461 taccggcatg atgtgcgcgg catcgcccgc cagcagtacg cgatcaatac ggaaacgttg
     5521 cgccagtcgc gcgttgtggg tgtagacacg ctggcgaatc aattcaacat tgtccggatt
     5581 aggcagcact ttgcttaaca gcttgcgcat attttgcggc tcacgcagct gttcttcggt
     5641 ttctcccggc atcaccataa attcaaagcg acgtaccgca tgaggcagcg cggcagaaac
     5701 atacgggcgc accggatcgc aacataaata gatatgcggc gtacttaacg gatcgttggc
     5761 gatatccacc acaatccact gatttggcgc agttttacct tcaaacggca cattcagggt
     5821 gcgacgaaca aaacttgccc caccatcaca ggccaccagc cactgggctt tgactatttc
     5881 ccgctgccct tctgccgttt tcaggtgcaa ggtcacttcg tcattttgct ggctgaaggc
     5941 ctccagctcg cgggaaaaca agcagcgcac attcggaaaa cgcgacaacc cttccagcat
     6001 caccgcatcg acctgaggct gaataaaggc gttacggcgc ggccagccaa attcatcggt
     6061 cattggctga atatcagcaa aacagcggcc tttcggggtg agaaaacgca tcgcgtgcca
     6121 cggcgtagtg tgcggcagaa catcatcgac caggccgacc gactgcatgg tgcgcagcgc
     6181 ctcgtcatca ataccaatcg cacgcgggta gtcgatcaac ttatcgagtt tctccaccac
     6241 cagcacgtca atgcccatct ggccgagata gttcgccatc atcagcccga ccggaccggc
     6301 accagcgatc gccacctgaa cgctatggtt aacagcaggc tggatgtcag ggtgttgtat
     6361 tgccatttca gtacctcacg actcggacaa aatgccgttg cgcgcacagt acggcgcaac
     6421 ttattttgtt aaaaacatgt aaatgatttt ttattgtgcg ctcagtatag gaagggtgtt
     6481 ttcggctaca atcaaaacat gcccgaatga gcaccaggtg cacaacgttg ttttaactat
     6541 agaaatgtca attaatatgc agaacaatga gcagacggaa tacaaaaccg tgcgcggctt
     6601 aacccgcggt ctaatgttat taaatatgtt aaataaactt gatggcggtg ccagcgtcgg
     6661 gctgctggcg gaactcagcg gcctgcatcg caccactgtg cggcgactgc tggagacgct
     6721 gcaggaagag ggatatgtcc gccgtagccc ctccgatgac agttttcgtc tgaccatcaa
     6781 agtgcggcaa ttaagcgaag gatttcgtga cgaacagtgg atttctgcac tggcggcccc
     6841 gctgctgggc gatctgttgc gcgaagtggt atggccaaca gatgtgtcca ccctggatgt
     6901 tgatgcaatg gtggtacgcg aaaccaccca ccgtttcagc cgcttatcct ttcaccgggc
     6961 aatggtcggg cgacgtttac cgctcctgaa aaccgcctcg ggcctgacct ggctggcctt
     7021 ttgcccggaa caagaacgca aggaattaat cgaaatgtta gccgcccgcc ccggtgatga
     7081 ctatcaactg gcacgagaac cgttaaagct gcaagccatt ctggcgcgcg cgcgcaaaga
     7141 gggttacgga cagaactacc gcggctggga tcaggaggag aagatcgcct ctatcgccgt
     7201 accgctgcgc agtgaacaac gggtgattgg ctgtctgaat ctggtgtata tggcgagcgc
     7261 aatgaccatt gaacaggcag cggaaaagca tcttccggcg ctacaacggg tagcaaaaca
     7321 gatcgaagaa ggggttgaat cgcaggctat tctggtggcc ggacggcgaa gcggcgtgca
     7381 tttacgttga caccatcgaa tggcgcaaaa cctttcgtgg tatggcatga tagcgcccgg
     7441 aagagagtca attcagggtg gtgaatgtga aaccagtaac gctatacgat gtcgcagagt
     7501 atgccggtgt ctcttatcag accgtttctc gcgtggtgaa ccaggccagc cacgtttctg
     7561 cgaaaacgcg agaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     7621 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     7681 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     7741 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     7801 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     7861 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     7921 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     7981 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
     8041 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
     8101 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     8161 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     8221 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     8281 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
     8341 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     8401 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     8461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8521 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8581 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tgaaattgtg
     8641 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt
     8701 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8761 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8821 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     8881 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
     8941 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
     9001 caatccgccg tttgttccca cggagaattc gacgggttgt tactcgctca catttaatgt
     9061 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
     9121 gtttcatctg tggtgcaacg gtcgctgggt cggttacggt caggacagtc gtttgccgtc
     9181 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
     9241 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
     9301 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
     9361 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
     9421 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
     9481 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtagttatg ccgatcgcgt
     9541 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
     9601 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
     9661 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
     9721 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
     9781 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
     9841 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
     9901 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
     9961 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
    10021 tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga
    10081 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
    10141 cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
    10201 ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga
    10261 gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt
    10321 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
    10381 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg
    10441 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc
    10501 cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt
    10561 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
    10621 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc
    10681 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga
    10741 gagagccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc
    10801 agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac
    10861 gctcccctcc gcgtcccata tcatcccgca actaaccacc agcgaaacgg atttttgcat
    10921 cgagctgggt aataagcgtt ggcaatttaa ccgtcagtca ggccttcttt cacagatgtg
    10981 gattggcgat gaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
    11041 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
    11101 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgct cggcagatac
    11161 acttgccgac gcggtgctga ttacgaccgc tcacgcgtgg ctgcatcagg gaaaaacctt
    11221 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
    11281 tgttgaagta gcgagcgata caccgcatcc ggcacggatt ggcctgacct gccagctggc
    11341 gcaggtcgca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
    11401 ccttactgcg gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
    11461 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
    11521 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
    11581 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
    11641 ccatatgggg attggtggcg acgactcctg gagcccgtca gtgtcggcgg aattccagct
    11701 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
    11761 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa aaaacacaaa
    11821 cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag cctacttccc
    11881 gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata cgggtattat
    11941 ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc tgctttctga
    12001 caaactcggg ctgcgcaaat acctgctgtg gattattacc ggcatgttag tgatgtttgc
    12061 gccgttcttt atttttatct tcgggccact gttacaatac aacattttag taggatcgat
    12121 tgttggtggt atttatctag gcttttgttt taacgccggt gcgccagcag tagaggcatt
    12181 tattgagaaa gtcagccgtc gcagtaattt cgaatttggt cgcgcgcgga tgtttggctg
    12241 tgttggctgg gcgctgtgtg cctcgattgt cggcatcatg ttcaccatca ataatcagtt
    12301 tgttttctgg ctgggctctg gctgtgcact catcctcgcc gttttactct ttttcgccaa
    12361 aacggatgcg ccctcttctg ccacggttgc caatgcggta ggtgccaacc attcggcatt
    12421 tagccttaag ctggcactgg aactgttcag acagccaaaa ctgtggtttt tgtcactgta
    12481 tgttattggc gtttcctgca cctacgatgt ttttgaccaa cagtttgcta atttctttac
    12541 ttcgttcttt gctaccggtg aacagggtac gcgggtattt ggctacgtaa cgacaatggg
    12601 cgaattactt aacgcctcga ttatgttctt tgcgccactg atcattaatc gcatcggtgg
    12661 gaaaaatgcc ctgctactgg ctggcactat tatgtctgta cgtattattg gctcatcgtt
    12721 cgccacctca gcgctggaag tggttattct gaaaacgctg catatgtttg aagtaccgtt
    12781 cctgctggtg ggctgcttta aatatattac cagccagttt gaagtgcgtt tttcagcgac
    12841 gatttatctg gtctgtttct gcttctttaa gcaactggcg atgattttta tgtctgtact
    12901 ggcgggcaat atgtatgaaa gcatcggttt ccagggcgct tatctggtgc tgggtctggt
    12961 ggcgctgggc ttcaccttaa tttccgtgtt cacgcttagc ggccccggcc cgctttccct
    13021 gctgcgtcgt caggtgaatg aagtcgctta agcaatcaat gtcggatgcg gcgcgagcgc
    13081 cttatccgac caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga
    13141 ataagagcag gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt
    13201 gggaaaacgt taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa
    13261 agcctgatta aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc
    13321 tatttctctt acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta
    13381 accattgtcg atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt
    13441 actctttccg ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac
    13501 tcttttccga taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca
    13561 ggcgtcacca tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt
    13621 ccaccaaacg tcgtggcggc tggcgttcct tgt
//
LOCUS       DAZFAH010000118        13530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFAH010000118 DAZFAH010000000
VERSION     DAZFAH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.118"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..757)
                     /gene="tcyN"
                     /locus_tag="ACHJWB_003209"
     CDS             complement(5..757)
                     /gene="tcyN"
                     /locus_tag="ACHJWB_003209"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4314788.1"
                     /translation="MSSIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(754..1422)
                     /gene="tcyL"
                     /locus_tag="ACHJWB_003210"
     CDS             complement(754..1422)
                     /gene="tcyL"
                     /locus_tag="ACHJWB_003210"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4314789.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1437..2423)
                     /gene="dcyD"
                     /locus_tag="ACHJWB_003211"
     CDS             complement(1437..2423)
                     /gene="dcyD"
                     /locus_tag="ACHJWB_003211"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4314790.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2528..3328)
                     /gene="tcyJ"
                     /locus_tag="ACHJWB_003212"
     CDS             complement(2528..3328)
                     /gene="tcyJ"
                     /locus_tag="ACHJWB_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4314791.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3416..3967)
                     /gene="fliZ"
                     /locus_tag="ACHJWB_003213"
     CDS             complement(3416..3967)
                     /gene="fliZ"
                     /locus_tag="ACHJWB_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4314792.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLEQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4013..4732)
                     /gene="fliA"
                     /locus_tag="ACHJWB_003214"
     CDS             complement(4013..4732)
                     /gene="fliA"
                     /locus_tag="ACHJWB_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4314793.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4898..6391)
                     /locus_tag="ACHJWB_003215"
     CDS             complement(4898..6391)
                     /locus_tag="ACHJWB_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO4314794.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSSLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     KRDGNIMSDANGKLYVKVAGSDKSPENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6646..8058
                     /gene="fliD"
                     /locus_tag="ACHJWB_003216"
     CDS             6646..8058
                     /gene="fliD"
                     /locus_tag="ACHJWB_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4314795.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGATPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLGEISK
                     AINDADSGSSASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     ITTEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSTSIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8073..8483
                     /gene="fliS"
                     /locus_tag="ACHJWB_003217"
     CDS             8073..8483
                     /gene="fliS"
                     /locus_tag="ACHJWB_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4314796.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEDSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8483..8848
                     /gene="fliT"
                     /locus_tag="ACHJWB_003218"
     CDS             8483..8848
                     /gene="fliT"
                     /locus_tag="ACHJWB_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4314797.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8926..10413
                     /gene="amyA"
                     /locus_tag="ACHJWB_003219"
     CDS             8926..10413
                     /gene="amyA"
                     /locus_tag="ACHJWB_003219"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4314798.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEHWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10447..10860)
                     /gene="yedD"
                     /locus_tag="ACHJWB_003220"
     CDS             complement(10447..10860)
                     /gene="yedD"
                     /locus_tag="ACHJWB_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4314799.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11047..12252
                     /gene="yedE"
                     /locus_tag="ACHJWB_003221"
     CDS             11047..12252
                     /gene="yedE"
                     /locus_tag="ACHJWB_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4314800.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12249..12482
                     /gene="yedF"
                     /locus_tag="ACHJWB_003222"
     CDS             12249..12482
                     /gene="yedF"
                     /locus_tag="ACHJWB_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4314801.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12591..13259
                     /gene="yedK"
                     /locus_tag="ACHJWB_003223"
     CDS             12591..13259
                     /gene="yedK"
                     /locus_tag="ACHJWB_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4314802.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQHV"
     gene            complement(13337..>13530)
                     /locus_tag="ACHJWB_003224"
     CDS             complement(13337..>13530)
                     /locus_tag="ACHJWB_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4314803.1"
                     /translation="KLLFMECNPIKEAFLMPWTLTGICYEISQRKYIVTNPDDYDKYS
                     DMVKKYVIDGEDHLIKYYM"
BASE COUNT         3419 a         3396 c         3287 g         3428 t
ORIGIN      
        1 cttattattg cagcagaaac ttctcgagga actggcgggt gcgaggctgc tggggatcgg
       61 caaataacgc ttttgctgcc ccctgctcga ctatccgccc ctggtccata aagatcgccc
      121 ggtccgcaac atcccgggca aagctcattt cgtgtgtcac aatcaccatc gtgcgctttt
      181 cctgcgccag ctggcggatg gtgttcagga cttcgcccac cagctctgga tccagcgccg
      241 acgttggctc gtcaaacaaa atcacctcag gacgcattgc cagcgcacgc gcaatcgcaa
      301 cacgctgctg ttgaccgcca gacaaacgac gtggatagct ggtttcttta ccggccagcc
      361 caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc ttctttcggt tcgcctttga
      421 cgatcaccgg cccttcaata atgttttcca gcaccgtacg atgcggaaac aaattaaagt
      481 tctggaagac aaacccgacg tgctgacgca actggcgaat cagcgatttt tgctgactta
      541 atgaacgtgc agtatcaata gtgatatcgc caacggtgat cgttcccgct tccggttgtt
      601 ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga accactcgga ccgataattg
      661 ccaccacttc gccaggcttt acctcgaggt cgataccgtg cagcaccgtc tgaccgtgaa
      721 attttttcac caggttctta acttcaatgg aactcatttt ggttctctct cctggcgatt
      781 aagttgattc tcaaaatggt tctgcaacgt cgataatact gtcgccatga tccagtagat
      841 gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg cgcgaggtaa tcaactgcgc
      901 ctgacggaac agctccggca cctggattgt cgcggccagc gacgtatcct ttaccaggct
      961 gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc gcctgtggca aaatcgcgcg
     1021 acgcatagtc tgccatggcg tcataccaat actggcagca gcttcccact gacctttatc
     1081 aatagaagaa atggcggcgc gcagtgtttc ggcggcatag gcggcagtgt tcagcgacag
     1141 accaatcatt gctgacggaa tgggatctaa ttcgatacca aactgcggca ggccgtaata
     1201 gatcataaac agttgtgcga tgagcggtgt accacgaaaa atagagatat aaaaacgcgc
     1261 cagccagcgc acgggccaga ttggcgacag gcgcatcagc gcgagaataa acccgagcag
     1321 taagccaaaa aacatgccgc cgatactgag ttgcagcgta tacccagccc ctttcagcag
     1381 gaacggcaag gaatcaataa ccagttgtat actttcttgc atgagcgttc ccggagctaa
     1441 acgtggggat gataggcgaa cagcgcaggc gcaccgccgg tatgaataaa cagaatcggc
     1501 ccttcatctt tgaagcgttt ctgactgata ccgtcaatca gccccgccat cgcttttccg
     1561 gtatacacag gatcaagcag aatgccttcc agccgcgcca gcaatttcac tgcttccatg
     1621 ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt catcccagag tataatttcc
     1681 gctgatgcgg tcagctccag ttctttcgca atcgcctgtt gtaggttaac cactttcggc
     1741 aactgatcgg caacggaacg cgacacggtc acgccaatca gttcgctttc aggcatcagg
     1801 tgttccagcc caacagccag tccggcgtga gttccggcac tgcccgatgc gaccaccacc
     1861 gacgaaatat taaccgcccc ttcacactgt tgcgcgattt ccagcgcact ctccacataa
     1921 cctagcgcgc caagagcatt agaaccgcca accggaatga catacgggcg aaagccttgt
     1981 gcttcgactc gcgtcgccag ctcttccagt tgggcattgg gatcggtcag tgcgtcgcac
     2041 atttcaatct gggtattgaa cagatccagc aacaaacgat tgccgttggt taaatagttt
     2101 tctgcggttg tgccaatagg attttccagc agcgccacgc agtgcagacc gagtttcgcc
     2161 gcgactgcgg cagtctggcg cacatggtta gactggatcg cgccggcagt aatcagcgta
     2221 tcggcacctt cgcgcagggc atctgccgcg agaaattcca gcttacgtaa tttattgccg
     2281 cccattgcca tgggtgtgac gtcatcccgt ttgatgaaaa tttcccgtcc aagataatca
     2341 gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga taaactccaa ccgtggaaaa
     2401 cgggttaaat tatgcagtgg cataacagcc tccgatgtgt gttgttgtga ttttcttatt
     2461 atgcacgctg aaaacgcgta aataaaaaag gcgctagtga aagcgccctt ttttgtcatt
     2521 atgctgatta tttggtcaca tcagcaccaa accatttttc ggaaagggct tgcaaagtgc
     2581 cgtctttttg catttccgca attgcatcat tcactgcttt cagcaggtct tcatttcctt
     2641 tacgcagcgc cacgccagac tcctgacggg agaatgcttc accggttact gccagcgtat
     2701 cgttggtttt cttcaccaga tccagcgccg ccagacgatc aacgaggatc gcatcgatac
     2761 ggcctacgcg cagatcctga tatttggtcg ggtcatcatc ataggtacgc acatcgacac
     2821 cctgaacatt ctgccgcagc cactcttcat agttggtgcc cagaccgaca cccacttttt
     2881 tgcctttcag atcagcagcc gttttaatgg tgccttcgtt accttttttc accagcgcct
     2941 gaataccaga aatggtgtac ggggttgaga aatcgtattt tttcttgcgc tcatcagaaa
     3001 tggtgacctg attaatcacc acatcaatac gtttagagtc cagcgacgcc agcataccgt
     3061 cccatttggt cggttttagg gacgcctcaa cgccaagatg ttttgccagc tgttgggcaa
     3121 attccacttc aaaaccggtt aatttgccgt catccccctg aaaactgaac ggcggataag
     3181 ttccttccag acctaccagc agcgtgccgc gctctttaac tttattaagc agaccttcat
     3241 ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac ggccatcaca cccatcaatg
     3301 cctgacgtcc cagatgtgct aatttcatat tcaccccgaa tgttgttatg tctgtttgca
     3361 gtgtagagcc attgtttgta aacacaaaaa caactccgct acatcttatt cttatttaat
     3421 atatatcaga agcaggcagg ctggaggatt tctgcacaag cccggtacac gtttgacgct
     3481 gatagtgttg atacttccgt aacgcaatgc ggtaattgtt ggtgctggta gtgggcagcc
     3541 acggggcgag gatttcatca aggaaaccgt cctgcagctg ttcgagggaa atattttgct
     3601 cgtgtagatg attacccaaa cggcgcaaac gaacgacata ttcacgcacc gtacctgggc
     3661 tcatttcagt ttgctcaaaa agaaattgct taaagccgat aatatcgaaa aaatcactct
     3721 gcgttttgca atgtaaatca ccgcagaatc ggcacaatgc cgcccatgat ttttgttccg
     3781 tttgccagcc attttcatca agcagcatgt ccaggcggga aatctcgatt ttattcacta
     3841 ttttgccgtc gcgaacgagg gtaatgcgat cgagtaattt acggcaatgc gcgcaatgag
     3901 tctggctgtg tttaaagtct ttaagatagc ggcttaatgg ccgtcttttc aggtgctgca
     3961 ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg catttactga cgttataact
     4021 tacccagttt agtgcgtaac cgtttaatgg cctgactgtg taactgactg acccgcgatt
     4081 ccccgacctc cagcaccgcg ccaatctctt tgagattcag ctcttcctgg taatagaggg
     4141 ttaataccag tttttcgcgc tccggcaacg tttcgatagc ttccatcacc cgctggcgca
     4201 gattactgtc cagcagttgt tgtagcgggt tttctcgctg atgatcatca gtaaccagtt
     4261 cgatgctatc gccgtgctct tcgcgccact catcgtagga gaagagctgg ctgttattgg
     4321 tgtcgagcaa catttgtcga taatcggcaa tatcgatccc taaacgttcc gctacctcag
     4381 tttccgtggc gttgcggcca agttcctgct ccagttgccc tattgcctgt gccacttcac
     4441 gcgcgttgcg acgcacgctg cgcggcaccc agtcacggct gcgaagttca tccagcatag
     4501 cgccacgaat acgctgcact gcgtaagttg taaatgccgt tccttgtaga gcgtcatagc
     4561 gttcgacggc attaagtaac ccaatgccgc ccgcctgtag cagatcgtca agttccacgc
     4621 tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg caccagcggg acataacgct
     4681 gccacagcga gtgtttatcc attacacctt cagcggtata gagtgaattc acgataaaca
     4741 gccctgcgtt atatgagtta tcggcatgat tatccgtttc tgcagggttt ttaatcggac
     4801 gattagtggg tgaaatgagg ggttatttgg gggttacggc tatttgccgg gtagaaaaaa
     4861 ccccgccgga gcggggtttg gcagaatata gcggcgatta acgcagcaga gacagtacgt
     4921 tctgcgtggt ctggttagcc tgtgccagaa cagaagtacc tgcttgttgc aggatctgcg
     4981 cacgagacat gttagacact tcggtcgcgt agtcagcatc ttcgatacgg ctacgggcag
     5041 aagacaggtt gtttacggtg ttgccaaggt tggtgatggc agagtcgaaa cggttttgaa
     5101 ttgcacctag gttagaacgc aacgagtcaa cctgagacag tgctttatcg atagcagcca
     5161 aaggatcttc tgttttctgc gtagaaattg ctgcaataga atcagttttc aggctcttac
     5221 cttgtaaatc actaacactg taagttttgc catcggttgc agttacaact tcggttttgc
     5281 catcgcttcc acccagttca actttaacat tgtcgtgttt gacaccgtca gcatcagtgt
     5341 aagacattgt tttaacggaa actgcacctg ttttctgatc aacattcgct gcatactgtt
     5401 taccgtcatc accaatcaac gcaaatgaac cagtatcttt gccattagcg tcttctaact
     5461 taaagagttt tgcacctgtg atagaagcgg ttgcggtatc agtacccaac tgaacatcaa
     5521 tagccccctt ccctttcacc gttgtgactt cctttaaatt acctgcttga ggctgggtac
     5581 ccgctagagc ccccagcttc agcttacctg catcaggaga tgtcggatca tcctccacag
     5641 taacttcata ataaccattt tcaggggatt tatctgaacc ggcaacttta acgtacaact
     5701 taccgttagc atcagacata atattaccgt cacgtttgcc cggagtcgta gtgcctttaa
     5761 ctgtagctgt aatatctgca tcaagtgtat tagcgtcagc aagcttaact gtcgatccaa
     5821 aataggttgt ttcagctgtt gcggctttta atttagtagg atctttaata ccaaaaccag
     5881 ataatcccag tgtgtcggaa ttaatttcct gcagtttaat agtaattgtt tcgccgtcgt
     5941 ttgcaccaac ctgaatagtc atatcctgat cagaagccag cactttaacg ccgttaaact
     6001 gagtctgacc agaaacacgg tcaatttcac tcagacgttg ctggatttcg gactggatgg
     6061 aggtcaggtc actgtcagag ttagtaccgt tagttgcctg aacagaaagt tcacgaatac
     6121 gctgcaggtt gttgttaatt tcattcagcg caccttcagt ggtctgcgca acagaaatac
     6181 catcattcgc gttacgggaa gcctgagtca gacctttaat atttgccgta aaacggttag
     6241 caatcgcctg acctgctgcg tcatctttag cactgttaat acgcaggcta gaagagagac
     6301 gttcaatggc ggagctcaga gaagactgag atttattcag gttattctgg gtcaacagcg
     6361 acaggctgtt tgtattaatg acttgtgcca tgatcttttc cttatcaatt acaacttgat
     6421 gttattgggc tgttgcccac ggtttctcac cgtacccctt gtatcggcac ctgaatttcg
     6481 aactttagaa aattttagtt tctctcctgt tttttcttaa cggctgaaat atccgtaata
     6541 tacaactcta ctctgcgcat catcttcgtg aatcaactat taaactttgc gcacaaattg
     6601 ccgataaccc gcttatctac tgtttgcaat caaaaggaat taggtatggc ttcatttaca
     6661 tctcttggcg ttggttcgaa tctgccgctg gatacattgc taaacaacct gaccatcgct
     6721 gaaaaaaaac gcttaaaccc aattactcag caacagagtg acaataccgc acgtctgaca
     6781 gcctatggca cattaaaaag tgcattagaa aaattccaga ctgcaaatac tgcgttaaat
     6841 aaagccgatc tgttcaaaag cactaccgtt accagtagca atgaagacct taaagtttcc
     6901 accgaagcag gtgctacccc cggtatctat accatcagcg taacccaact ggcgcaggcg
     6961 caatctttac gaactgattc cccgacaatc attgcatcaa caaaagatgc actgggtgat
     7021 gagtcatcag atacacgcac aataaaaatt acccaagacg gccgtaaaga gccgctggaa
     7081 atcaagctca ataaagatca gacgtcattg ggtgagatca gcaaagccat taacgatgcc
     7141 gatagtggaa gttccgctag tatcgttaaa gtcaaagatg gtgactatca attggtattg
     7201 accgccagtg aaggtctggc caataaaatg accatatctg ttgagggtga cagtaaatta
     7261 aatgatctgc tggcttatga cagcaagacc aacaccggga atatgaagga actggttaac
     7321 gcgcaaaatg cacagctaaa cgttaacggt atcgatattg aacgttcaag taacaaaatt
     7381 accgatgctc ctcagggagt gactctcgat ttgaccaaaa aagtcactga cgtccgggtc
     7441 acggtgacaa aaagcaacga taaagcaacc gaagcaatta aaggctgggt ggattcatat
     7501 aactccctca ttgacacctt taacaccctg acaaaatata aagaagtaga cccaggtgct
     7561 gaagcacaag ataaaaataa tggtgcactt ctcggagaca gcgttgtacg tacaattcaa
     7621 agcggcatcc gcgcacaatt tgcaaacggt gccagcgacg gtgccttcaa aacattaaac
     7681 gagatcggga ttaagcagga cggcacgacg ggtaagctga aaattgatga tgataaactc
     7741 aaaaaagtcc tgaatgaaaa tacagcatcc gtacgtgaat tgctggtagg cgatggtaaa
     7801 gaaacaggaa ttaccaccaa aattaccacc gaggtaaaag gttaccttgc cgatgatggc
     7861 attattgata gcgcacagga cagtattaac gccacactga aaaaactgac caaacagtat
     7921 ctatccgtca gtaccagcat tgatgacacc gtggcgcgtt acactgcaca gtttacgcaa
     7981 ttagatacca tgatgagcaa actgaataac accagtacct atctcagcca acaatttact
     8041 gcaatgagta attcctgatc acggaggtca gcatgtatac cgcgagcggc tcaaaagcct
     8101 atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca
     8161 tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca
     8221 atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggactgc
     8281 gggtgagtct tgatgaagat agcaaagacg aattaaccca aaacttgatt gctctttata
     8341 gctatatggt caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag
     8401 aagtggaagc attaatgcgt aatattgccg atgcctggaa agagtcgtta ctctcccctt
     8461 ctttgattca ggacccagtc tgatgaacca tgcaccgcat ttatatttcg cctggcaaca
     8521 actcgtcgaa aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact
     8581 catcgccagc gaaatggcgt atgttaatgc ggtgcaggag attgcacatt tgactgaaga
     8641 ggttgacccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga
     8701 caacgaaagc aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt
     8761 cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt
     8821 gctggctccg caggataacc tcttttgaat ctgaatgagt cgatggctcg cgaataatcc
     8881 gattacggct acgcttctaa tattcccctt gaatggagtc gaagaatgcg taatcccacg
     8941 ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc tgaattggcc
     9001 gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat
     9061 aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggt
     9121 gagtttgatc agaaaggcag catccctact aaatatggcg ataaagtaca actgctggcc
     9181 gccattgacg ccctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac
     9241 aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt
     9301 acgcaaattg atgaagaaat cattgagtgc gaaggctgga cgcgttacac cttccccgcc
     9361 cgtgccgggc aatactcaca gtttatctgg gatttcaaat gttttagcgg tatcgaccat
     9421 atcgaaaatc ctgatgaaga tggcatattt aaaattgtta acgactacac cggcgaaggc
     9481 tggaacgatc aggttgatga tgaattaggt aatttcgatt atctgatggg tgagaatatc
     9541 gatttccgca atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa
     9601 caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccagc ctggttttat
     9661 aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcggag
     9721 cactggtcgc atgaagttga taagctgcaa acgtatattg atcaggtgga aggcaaaacc
     9781 atgctgtttg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat gggacgcgac
     9841 tacgacatga cgcagatttt cactgggacg ctggtggaag ccgatccttt tcacgccgta
     9901 accctcgttg ccaaccatga cacccaaccg ttgcaagccc tcgaagcgcc agtcgaaccg
     9961 tggtttaaac cgctggcgta cgccctaatt ttgttgcggg aaaatggcgt tccttcggta
    10021 ttctatccgg acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat
    10081 ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc
    10141 gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt
    10201 ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga atggggatga tggcgaaaaa
    10261 accattcatc tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaaccgg
    10321 caagagagcg tagtgaccga cgaaaacggc gaagcaacct tcttttgcaa cggcggtagc
    10381 gtcagcgtgt gggttatcga agaggtgatt taaatttatc cccggcggca agccggggag
    10441 atttcattac ggcaatggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc
    10501 cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg
    10561 ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt
    10621 caacttaccc ggcacggcaa tcacccgctg ccactgacgg caatccaggg tatcgccctc
    10681 tttagtgaca atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt
    10741 ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt
    10801 ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat
    10861 cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc
    10921 tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa
    10981 ataaatttgc atggtgtatg attcgcgact cttgtttcaa accttctgag ttcagaggct
    11041 acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg ggcgcccatc
    11101 cccgcggtca tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt
    11161 tgggctgtca cgggtgaatt tacccgttgg ggcggccagc tcctgcaact gttcggcgtc
    11221 catgctgaag agtggggtta ctttaaaatt atccatctgg aaggatcgcc attaacccgc
    11281 atcgacggga tgatgatcct cggtatgttt ggcggctgct ttgccgcagc gctgtgggcc
    11341 aacaatgtca aactgcgaat gccgcgcagc cgtatccgca ttatgcaggc catcattggc
    11401 ggcattatcg ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt
    11461 accggtattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt
    11521 tcatggtttg gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag
    11581 aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt
    11641 ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc tgctcacagc gatgaaccaa
    11701 cccaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgca
    11761 caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg
    11821 aaagcaatca ttattggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta
    11881 ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt
    11941 ggttttggca tcgtgctggc tggcggctgc gaaaccggct ggatgtaccg cgcggtagaa
    12001 ggccaggtgc actactggtg ggtcggtctg ggcaacgtga tcggctcaac gattctggcg
    12061 tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg
    12121 aaaacctttg gtccgatggg tggcctgctg gtgacatatt tgctgttgtt tgctgcgctg
    12181 atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag
    12241 gagatcgcat gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc
    12301 cttatccggc agtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg
    12361 aagtggtgag cgactgtccg cagtcgatca ataatattcc actggatgcg cgtaatcacg
    12421 gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt
    12481 aatccctcat tcccgctgtg acccacagcg ggaacatcgt tacctttgta tcacatagat
    12541 tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggtttct atgtgtggac
    12601 gctttgccca atcccaaacg cgtgaagatt accttgcgct tctcgcggaa gatattgaac
    12661 gcgatattcc ctatgatcct gaacccattg gcagatacaa cgtcgcgccg ggaaccaaag
    12721 tccttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg
    12781 ctcccggatg gtgggataaa ccgccactga ttaacgcccg cgtagaaact gccgcaacga
    12841 gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
    12901 ttgagtggaa aaaagaaggc gacaaaaagc agccttattt tatctatcgt gctgatggac
    12961 aacctgtttt tatggccgcg ataggcagca caccgttcga gcgcggtgac gaagccgaag
    13021 gatttttgat tgtcactgca gcagcagacc agggtctggt agatattcat gaccgccgcc
    13081 cactggtact gtcgccagaa gctgcacggg aatggatgcg gcaagagatt ggcggtaaag
    13141 aagcatcaga aattgctacc aatggctgtg tgccagctaa ccagttcacc tggcacccgg
    13201 tatcgcgcgc ggtgggtaat gttaaaaacc agggcgcgga gttaattcaa catgtttgat
    13261 aagcctggag attagtgatg aacaaaaatg tgtcacaaga aacctctatt ccagtgacac
    13321 aattgcgcct aattacttac atataatatt taattaggtg atcctcacca tctattacat
    13381 actttttaac catatcggaa tatttatcat aatcatcggg gttcgtaaca atatattttc
    13441 gctgcgatat ttcatagcaa atccctgtaa gagtccatgg cattaaaaat gcctctttaa
    13501 taggattaca ctccatgaaa agtaatttta
//
LOCUS       DAZFAH010000119        13475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFAH010000119 DAZFAH010000000
VERSION     DAZFAH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.119"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..644
                     /gene="yahL"
                     /locus_tag="ACHJWB_003225"
     CDS             <1..644
                     /gene="yahL"
                     /locus_tag="ACHJWB_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4314804.1"
                     /translation="DDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1056..1301
                     /gene="yahM"
                     /locus_tag="ACHJWB_003226"
     CDS             1056..1301
                     /gene="yahM"
                     /locus_tag="ACHJWB_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO4314805.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(1318..1989)
                     /gene="yahN"
                     /locus_tag="ACHJWB_003227"
     CDS             complement(1318..1989)
                     /gene="yahN"
                     /locus_tag="ACHJWB_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4314806.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2136..2411
                     /gene="yahO"
                     /locus_tag="ACHJWB_003228"
     CDS             2136..2411
                     /gene="yahO"
                     /locus_tag="ACHJWB_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4314807.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2509..4095)
                     /gene="prpR"
                     /locus_tag="ACHJWB_003229"
     CDS             complement(2509..4095)
                     /gene="prpR"
                     /locus_tag="ACHJWB_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4314808.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQAPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            4333..5223
                     /gene="prpB"
                     /locus_tag="ACHJWB_003230"
     CDS             4333..5223
                     /gene="prpB"
                     /locus_tag="ACHJWB_003230"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4314809.1"
                     /translation="MSLHSPGKAFRGALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            5268..6437
                     /gene="prpC"
                     /locus_tag="ACHJWB_003231"
     CDS             5268..6437
                     /gene="prpC"
                     /locus_tag="ACHJWB_003231"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4314810.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYYIADRLETVMWETKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6471..7922
                     /gene="prpD"
                     /locus_tag="ACHJWB_003232"
     CDS             6471..7922
                     /gene="prpD"
                     /locus_tag="ACHJWB_003232"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4314811.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7962..9848
                     /gene="prpE"
                     /locus_tag="ACHJWB_003233"
     CDS             7962..9848
                     /gene="prpE"
                     /locus_tag="ACHJWB_003233"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4314812.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVFLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMAQV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(9992..10891)
                     /gene="cynR"
                     /locus_tag="ACHJWB_003234"
     CDS             complement(9992..10891)
                     /gene="cynR"
                     /locus_tag="ACHJWB_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4314813.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMANFYARYPGITLQQQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            11001..11660
                     /gene="cynT"
                     /locus_tag="ACHJWB_003235"
     CDS             11001..11660
                     /gene="cynT"
                     /locus_tag="ACHJWB_003235"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4314814.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            11691..12161
                     /gene="cynS"
                     /locus_tag="ACHJWB_003236"
     CDS             11691..12161
                     /gene="cynS"
                     /locus_tag="ACHJWB_003236"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4314815.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            12167..13348
                     /gene="cynX"
                     /locus_tag="ACHJWB_003237"
     CDS             12167..13348
                     /gene="cynX"
                     /locus_tag="ACHJWB_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4314816.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
BASE COUNT         3161 a         3521 c         3655 g         3138 t
ORIGIN      
        1 gtgatgattt agtcgatgac gacaaactca ttcatctcat ggctgccgta cgagactgtg
       61 agtggtcaga cgataacgca ctcaccataa atgtgcagtt taatgatttc ccaggatttt
      121 atgactggat ggattaccct gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc
      181 aaaaagggac agtttgggta tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg
      241 tgcaggctgg acgctttacc gggcttaaga aattagtgca gtttatcgat tcagtgcgaa
      301 cagattgcaa atgtattctt cttgaacacc atatgccttt gctgagaatt ttccctaaag
      361 ggaaagagtg tatgcgtgtg gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag
      421 acgcgcccat taagcaggcg cttgcgcatg gattattatt acacttaaaa aatatccacc
      481 ctgtttttcc tgaatcactg gtgatgttat tgctctctgt tctggacgta aaaacataca
      541 gagatgacgc acgattaaat gaatggatat cgaatagagt gcaggaatta ggtgacagat
      601 attatcctgt aaataagcac gttaaaatcc gctacactct ttagttcaga aagggttcca
      661 cctgcaagtt gttattccac cagagagtga atcgcaaagc aggtaatcat ttcatctgta
      721 tatccgtatg ccaaatggca ttccaccgcc ttttcgccga tattttgttg tgatcggcga
      781 cacttcgctt aaaaaagcac cagcagtggt ttcgcagcca tgcggtgtat aaaaaatgat
      841 ctcatgcaga tgttttgtga atgtgtgggt tgacattcat atgaaaaaaa tcataattcc
      901 atcatgtttg tgtggggtct tttctgtatc ttacgcatcg cactcaagcc tgacagaaaa
      961 tatgctgtaa ggctcatatc aaaaaccgcc attagctcat caggaagagc agacgacaac
     1021 caggattgtt gtatggtacg gggttcgagg cctcgatggc ggtccaactt ttcaaaactc
     1081 ttctgaatca gatcccctta ttatcgtccc tgcaaagtgg aacattgcca ttgtttggct
     1141 attcaggacg ggtcagacca atgaaaaaag cccacacagg ggagagtggg ctgaaatggg
     1201 aagctaaaga ctcaagtaaa cttatcggaa ataaggacca cgcattacgg gggctatcat
     1261 cgccgatggc cgggattcgg caaatcaggt tgattacgta gagagtaaat tatctgctca
     1321 ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat acaccaataa ttgcaccaat
     1381 aacccgactg gcaacgcgtt gcatacgccc ataagcacga cgcacagcgg gcaaagaaaa
     1441 cgcctgacta agaaaaactc gccagataat tgatgcgagc acaatccccg cccaggccat
     1501 taaacgtgcc catgttggtg tttcggcatt taatgttact gagaaaatac tgataaaaaa
     1561 taaaacggtt tgcgggttag agagatcggt aattaatccg cggcgaaaaa agacatacca
     1621 gggggcgcta atcggttgtt gtagtgtgct catttgcggt gttgactggc ggcgcatgct
     1681 gcaccacgca aaccataaga gataagcgcc gccgacgatt ctgataagcg aaaaaatctc
     1741 ctcacactgc gtaattagcg ttgcaagacc aaacaaaccc aacccggaat aaaatgcatc
     1801 gcccagcgcc acgcccagcc cggtcagcac ccctgcgcgt cgaccggaag ccaggctggt
     1861 ttgtactacc acaaagagat tggctcccgg attaaaaaaa gtaatcacga acagtcctac
     1921 ggtcaggtaa acggcgtgca aaggatccat agtgatttca tccataaata agtgaagtaa
     1981 cttcatcata tttctaccaa aaataatcgg gtgcgagaga gatcacaaag tgtcttattt
     2041 ccggttactg gcgtttatgc cctgactgaa ctaattatta atcaacccaa taatgtgggt
     2101 gggtgatagt gtgataacaa ctctggagcc gtaatatgaa aataatctct aaaatgttag
     2161 tcggtgcgtt agcgttagcc gttaccaatg tctatgccgc tgaattgatg accaaagcgg
     2221 aatttgaaaa agttgaatcg cagtatgaaa aaataggtga tatttcaacc agcaatgaaa
     2281 tgtcgactgc agacgcgaaa gaagatttga tcaaaaaagc ggatgaaaaa ggggctgatg
     2341 tgttggtact gacctccggt caaactgaca ataagatcca cggcacggca aatatttata
     2401 agaagaagta attctgaacc ctatgtaaac atgcccgatg cgtaagttta tcggtgatca
     2461 tcaattgaaa tttatgccgg ataaagcgtt cgcgctgcat tcggcagttc agcttttcag
     2521 ccgccgccag aacgtcgtcc ggctgatgcc taaataattc gccgctgctg ttttatcgcc
     2581 attaaatttc tccagtgcct gttgtggtgt cagtaagcgt ggagcgggag ttttcgccga
     2641 ctcgcgcgcc agttccggca gtagcagctg caaaaattgc ggcgttaaat ccggcgtcgg
     2701 ttccacactt aaaaatagcg ccagtcgctc catcatattg cgcagttcac gaatattacc
     2761 tggccagtcg tagtgcacca gcacggtttc gcttgcctgt aatccctggc gtaatgcggc
     2821 agaaaacggg gcggagagcg ccgccagaga cactttcaaa aagctttccg ccagcggaag
     2881 aatatccgcc acccgctcgc gcagtggtgg caattgcaga cgcaaaatac tcagccgata
     2941 aaacagatca cggcgaaaac gtccttgctg catatcttct tccagcttgc agtgagtggc
     3001 gctaatgacc cgtacatcta ccggaacagg ctgatgcccg ccgacgcggg tgacctcttt
     3061 ttcttccagc acccgtaaca ggcgagtctg caaaggtagc ggcatttcgc caatctcatc
     3121 cagaaacagc gtaccgccgt gggcaatttc gaacagcccg gcgcgacctc cgcgtcgcga
     3181 gccggtaaac gccccttcct catagccaaa cagctccgct tccagcagcg attcggctat
     3241 cgccccgcag ttgacggcaa caaacggatg cgactttttg ccctgtcgcg catcgtggcg
     3301 ggcaaaatat tcccgatgaa tcgcctgggc cgccagctct ttgcccgtcc ccgtttcccc
     3361 ctcaatcaac accgctgcac tggagcgggc atacagcaaa atagtctgcc gtacttgttc
     3421 catctgtggt gcttgaccga gcatatcgcc cagcacgtaa cgagttctca gggcgttgcg
     3481 ggtggcatcg tgagtgttat ggcgtaacga catgcgcgtc atatccagcg catcgctgaa
     3541 cgcctggcgc acggtggcgg cggaatagat aaaaattccg gtcattccgg cttcttctgc
     3601 cagatcggta atcagccccg cgccgaccac cgcttcagtg ccgttagctt tcagctcgtt
     3661 aatctgcccg cgggcgtctt cttcggtaat gtagctacgt tgatcgaggc gcagattaaa
     3721 ggttttttga aacgccacca gcgctggaat ggtttcctga taggtgacaa cgccgataga
     3781 agaggtgagt tttccggctt ttgccagtgc ctgtaacaca tcgtagccgc tcggtttaat
     3841 caaaataact ggcactgaca ggcggctttt caggtacgcg ccgttagagc cagcagcgat
     3901 gatggcgtca cagcgttcgt ttgccagttt cttgcggatg taggtcactg ctttttcaaa
     3961 gccaagctga ataggggtaa tgttcgccag gtgatcaaac tcgaggctga tatcgcgaaa
     4021 cagctcgaac aggcgcgtta cagataccgt ccagataacc ggtttgtcgt cattaagccg
     4081 tggtggatgt gccatagcgc accgcaaagt taagaaaccg aatattgggt ttagtcttgt
     4141 ttcataattg ttgcaatgaa acgcggtgaa acattgcctg aaacgttaac tgaaacgcat
     4201 atttgcggat tagttcatga cgttatctct aacaaattga aattaaacat ttaattttat
     4261 taaggcaatt gtggcacacc ccttgctttg ttttattaac gaaaataaca agttgataac
     4321 aaaggatggg ctatgtctct acactctcca ggtaaagcgt ttcgcggtgc actgactaaa
     4381 gaaaatccat tgcagattgt tggcaccatc aacgctaatc atgcgctgtt ggcgcagcgt
     4441 gccggatatc aggcaattta tctctccggc ggtggcgtgg cggcagggtc gctggggctg
     4501 cccgatctcg gtatttctac ccttgatgat gtgctgacag atattcgccg tatcaccgac
     4561 gtttgttcgc tgccactgct ggtggatgcg gatatcggtt ttggttcttc ggcctttaac
     4621 gtggcgcgta cggtgaaatc gatgattaaa gccggtgcgg caggattgca tattgaagat
     4681 caggttggtg cgaaacgctg cggtcatcgc ccgaacaaag cgatcgtctc gaaagaggag
     4741 atggtggatc ggatccgcgc ggcggtggat gcgaaaaccg atcctgattt tgtgatcatg
     4801 gcgcgcaccg atgcgctggc ggtagagggg ctggatgcgg cgatcgagcg tgcacaggcc
     4861 tatgttgaag cgggtgccga gatgttgttc ccggaggcga ttaccgaact cgccatgtac
     4921 cgccagtttg ccgatgcggt gcaggtgccg atcctcgcca acattaccga atttggcgca
     4981 acaccgctgt ttaccaccga cgaattacgc agcgcccatg tcgcaatggc gctgtaccca
     5041 ctttcagcgt tccgcgccat gaaccgcgcc gctgagcatg tctacaacgt cctgcgccag
     5101 gaaggcacgc agaaaagcgt catcgatacc atgcaaaccc gcaacgagct gtacgaaagc
     5161 atcaactact accagtacga agcgaagctc gacgacctgt ttgcccgtag ccaggtgaaa
     5221 taaaacacaa cgaaaaatat ctcgacccta caaatgacga ggacaacatg agcgacacaa
     5281 cgatcctgca aaacagtacc catgtcatta aaccgaaaaa atctgtggca ctttctggcg
     5341 ttccggcggg caatacggcg ctctgcaccg tgggtaaaag cggcaatgac ctgcattacc
     5401 gcggctacga tattcttgat ctggcggaac attgcgaatt tgaagaagtg gcgcatctgc
     5461 tgatccacgg caaactgccg acccgtgacg aactcgccgc ttacaaaacg aaactgaaag
     5521 ccctgcgcgg tttaccggct aacgtgcgta ccgtgctgga agccttaccg gcggcgtcac
     5581 acccgatgga tgttatgcgc accggcgttt ccgcgctcgg ctgcacgctg ccagaaaaag
     5641 agggacatac cgtctctggc gcgcgggata ttgccgacaa actactggca tcacttagct
     5701 cgattctcct ttattggtat cactacagtc acaacggcga acgcatccag ccggaaaccg
     5761 atgacgactc catcggtggt cacttcctgc atctgctgca cggcgaaaag ccatcgcaaa
     5821 gctgggaaaa ggcgatgcat atttcgctag tgctgtacgc cgaacacgag tttaacgcct
     5881 ccacctttac cagtcgggtg attgcgggca ctggctctga tatgtattcc gcgattattg
     5941 gcgcgattgg cgcactgcgc gggccgaaac acggcggagc gaatgaagtg tcgctggaga
     6001 tccagcaacg ctacgaaacg ccggacgaag ccgaagccga tatccgcaag cgggtggaaa
     6061 acaaagaagt ggtcattggt tttggtcatc cggtttatac catcgccgac ccgcgccacc
     6121 aggtgatcaa acgtgtggcg aagcagctct cgcaggaagg cggctcgttg aagatgtact
     6181 acatcgccga tcgcctggaa acggtgatgt gggaaacgaa aaagatgttc cccaatctcg
     6241 actggttctc cgctgtttcc tacaacatga tgggcgttcc caccgagatg ttcacaccac
     6301 tgtttgttat cgcccgcgtc accggctggg cggcgcacat tatcgaacaa cgtcaggaca
     6361 acaaaattat ccgtccttcc gccaattatg ttggaccgga agaccgccag tttgtcgcgc
     6421 tggataagcg ccagtaaacc tctacgaata acaataagga aacgtaccca atgtcagctc
     6481 aaatcaacaa catccgcccg gaatttgatc gtgaaatcgt tgatatcgtc gattacgtca
     6541 tgaactacga aatcagttct aaagtggcct acgacaccgc acattactgc ctgctcgaca
     6601 cgctcggctg cggtctggaa gctctcgaat acccagcctg taaaaaactt ctggggccaa
     6661 ttgtccccgg caccgtcgta cccaacggcg tgcgcgtccc cggaactcag tttcagctcg
     6721 accctgtcca ggcggcattt aacattggcg cgatgatccg ctggctcgat ttcaacgata
     6781 cctggctggc ggcggagtgg gggcatcctt ccgacaacct cggcggcatt ctggcaacgg
     6841 cggactggct ttcgcgcaac gcggtcgcca gcggcaaagc gccgttgacc atgaaacagg
     6901 tgctgaccgc aatgatcaaa gcccatgaaa ttcagggctg catcgcgttg gagaattcct
     6961 ttaaccgcgt cggcctcgac cacgttctgt tagtgaaagt ggcttccacc gccgtggtcg
     7021 ccgaaatgct cggcctgacc cgcgaggaaa ttctcaacgc cgtttcgctg gcgtgggtgg
     7081 acggtcagtc gctgcgcacc tatcgccatg cgccgaacac cggcacgcgt aaatcctggg
     7141 cggcgggcga tgccacttcc cgcgcggtac gtctggcact gatggcgaaa acgggcgaaa
     7201 tgggttaccc gtcagccctg accgcgccgg tatggggctt ctacgacgtc tcctttaaag
     7261 gtgaatcgtt ccgcttccag cgtccgtacg gttcttacgt catggagaat gtgctgttca
     7321 aaatctcctt cccggcggag ttccactccc agacggcagt tgaagcggcg atgacgctct
     7381 atgaacagat gcaggcagca ggcaagacgg cggcggatat cgaaaaagtg accattcgca
     7441 cccacgaagc ctgtattcgc atcatcgaca aaaaggggcc gctcaataac ccggctgacc
     7501 gcgaccactg cattcagtac atggtggcga tcccgctgtt attcgggcgc ttaacggcgg
     7561 cagattacga ggacaacgtt gcgcaagata aacgcatcga cgccctgcgc gagaagatca
     7621 attgctttga agatccggca tttaccgctg actaccacga cccggaaaaa cgcgccatcg
     7681 ccaatgccat tacccttgag ttcaccgacg gcacacgatt tgaagaagtg gtggtggagt
     7741 accccattgg tcatgctcgc cgccgtcagg atggtattcc gaaactggtc gataaattca
     7801 aaatcaatct cgcgcgccag ttcccgactc gccaacagca gcgcattctg gaggtttctc
     7861 tcgacagaac tcgcctggaa cagatgccgg tcaatgagta tctcgacctg tacgtcattt
     7921 aagtaaacgg cggtaaggcg taagttcaac aggagagcat tatgtctttt agcgaatttt
     7981 atcagcgttc gattaacgaa ccggagcagt tctgggccga gcaggcccgg cgtattgact
     8041 ggcagacgcc ctttacgcaa acgctcgacc acagcaatcc gccgtttgcc cgttggtttt
     8101 gtgaaggccg aaccaatttg tgccacaacg ctatcgaccg ctggctggag aaacagccag
     8161 aggcactggc attgattgcc gtctcttcgg aaacagaaga agagcgcacc tttacctttc
     8221 gccagctgca tgacgaagtg aacgcggtgg cctcaatgtt gcgctcactg ggcgtgcagc
     8281 gcggcgatcg ggtgctggtg tatatgccga tgattgccga agcgcatatt actctgctgg
     8341 cctgcgcgcg cattggtgct attcattcgg tggtatttgg tggttttgcc tcgcacagcg
     8401 tggcggcgcg aattgatgac gctaaaccgg tgctgattgt ctcggctgat gccggagcgc
     8461 gcggtggcaa aatcattccc tataaaaaat tgctcgacga tgcgataagt caggcgcagc
     8521 accagccacg ccatgttttt ctggtggatc gcgggctggc gaaaatggcg cgcgtcagcg
     8581 ggcgggatgt cgatttcgcg tcgttgcgcc atcaacatat cggcgcgcgg gtaccggtgg
     8641 cgtggctgga atccaacgaa acctcctgca ttctctacac ttccggcacg accggcaaac
     8701 ctaaaggcgt gcagcgtgac gtcggcggat atgcggtggc gctggcgacc tcgatggaca
     8761 ccatttttgg cggcaaagcg ggcagcgtgt tcttttgcgc atcggatatc ggctgggtgg
     8821 tggggcattc gtatatcgtc tacgcgccgc tgctggcggg gatggcgact atcgtttacg
     8881 aaggattgcc gacctggccg gactgcggcg tgtggtggaa aatcgtcgag aaatatcagg
     8941 ttagccggat gttctcagcg ccgaccgcca ttcgcgtgct gaaaaaattc cctaccgctg
     9001 aaattcgcaa acacgatctc tcgtcgctgg aagtgctcta tctggctgga gaaccgctgg
     9061 acgagccgac cgccagttgg gtgagcaata cgctggatgt gccggtcatc gacaactact
     9121 ggcagaccga atccggctgg ccgattatgg cgattgctcg cggtctggac gacaggccga
     9181 cgcgtctggg aagccccggc gtgccgatgt atggctataa cgtgcagttg cttaatgaag
     9241 tcaccggcga accgtgtggc gtcaacgaga aagggatgct ggtggtggaa gggccgctgc
     9301 cgccgggctg tattcagacc atctggggcg acgacgaccg ctttgtgaag acttactggt
     9361 cgctgttttc ccgcccggtg tacgccacct ttgactgggg catccgtgac gctgacggtt
     9421 atcactttat tctcgggcgc actgacgatg taattaacgt tgccgggcat cgtctgggca
     9481 cgcgcgagat tgaagagagt atctccagcc atccgggcgt tgccgaagtg gctgtggttg
     9541 gggtgaaaga tgcgctgaaa gggcaggtgg cggtggcgtt tgtcattccg aaagagagcg
     9601 atagtctgga agatcgtgat gtggcgcatt cgcaagagaa ggcgattatg gcgcaggtgg
     9661 acagccagat tggcaacttt ggtcgcccgg cgcacgtctg gtttgtctcg caattgccga
     9721 aaacgcgatc cggaaaaatg ctgcgccgca cgatccaggc aatttgcgaa ggacgcgatc
     9781 ctggggatct gacgaccatt gatgatcctg cgtcattgga tcagatccgc caggcgatgg
     9841 aagagtaagg tattgtcgga tgcgtcgcgt ggcgcatccg acaccatgcg ccgatgcctg
     9901 atgcgacgct gacgcgtctt atcagtatct acgtagagca ctgaactcgt aggcctgata
     9961 agcgtagcgc atcaggcaat tgtgtcagtg gttaccgcga ttcatttccg ccaacaaccg
    10021 cgcatttatc caacgccatg tgcaaaaatg ccttcgcggc ggctgtctgc cagctatttt
    10081 tccgccgcaa caaaaccgcc gttctctcca gtagtggcgg ggcaagagaa atcgctttaa
    10141 gcccgtcatg ttgtgtggca atcgctgctg gtaacaatgt ggaaagggaa gtgcggcgaa
    10201 tcagctccag aaccgcgcta attgagttcg cctcaatgac cacctgtgga tgcagccccg
    10261 ctttctcgca gtagtggtca atttgctctc tggtggcaaa ttccgcgctg agcagtacca
    10321 gtttttcatc atgcaagcga ctcaacgcca cctgttcaca ggcagccagc ggatgatgtt
    10381 gcgccacgac taacgctaaa ctttctgtca gtaaaggaat tgcctccagc tccggcgaat
    10441 gcacaggcgc gaaggcaatc ccaacgtcca actcgtcgcg acaaagcaga tcttcgattt
    10501 tctcctgcga catttcctgt tgctggagcg tgatgccggg gtagcgcgca tagaaattcg
    10561 ccattaaagg gccgataaag tagcttgtaa aggtgggagt gacggcgata cgcagcgatc
    10621 ctcgcgtcag atcggcaaca tcatgaatcg cccgtttacc cgcccccagt tcctgtaacg
    10681 cccggctggc gtactgtcgc cagacttctc ctgcatcagt gagacgaatc gttcgcccgc
    10741 tacggtcaaa cagcggcacg cctaaactct cctctaactg gcgaatctgc tgggaaagcg
    10801 caggttggga gacgtgcaac gcactggctg cacgggtgaa gctgccatgt tcagccacgg
    10861 caagaaagta attgatatgt cgagagagca ttcgccaacc tataagtaaa tccaatagaa
    10921 ctcatcataa atgagacttt taccttatga caatcggcga gtagtctgcc tctcattcca
    10981 gagacagaca gaggttaacg gtgaaagaga ttattgatgg attccttaaa ttccagcgcg
    11041 aggcatttcc gaagcgggaa gccttattta aacagctggc gacacagcaa agcccgcgca
    11101 cactttttat ctcctgctcc gacagccgtc tggtccctga gctggtgacg caacgtgagc
    11161 ctggcgatct gttcgttatt cgcaacgcgg gcaatatcgt cccttcctac gggccggaac
    11221 ccggtggcgt ttctgcttcg gtggagtatg ccgtcgctgc gcttcgggta tctgacattg
    11281 tgatttgtgg tcattccaac tgtggcgcga tgaccgccat tgccagctgt cagtgcatgg
    11341 accatatgcc tgccgtctcc cactggctgc gttatgccga ttcagcccgc gtcgttaatg
    11401 aggcgcgccc gcattccgat ttaccgtcaa aagctgcggc gatggtacgt gaaaacgtca
    11461 ttgctcagtt ggctaatttg caaactcatc catcggtgcg cctggcgctc gaagaggggc
    11521 ggatcgccct gcatggctgg gtctacgaca ttgaaagcgg tagcatcgca gcttttgacg
    11581 gcgcaacccg ccagtttgtg ccactggccg ctaatcctcg cgtttgtgcc ataccgctac
    11641 gccaaccgac cgcagcgtaa ccttattttt aaaccatcag gagatccacc atgattcagt
    11701 cacaaattaa ccgcaatatt cgtcttgatc ttgccgatgc cattttgctc agcaaagcta
    11761 aaaaagatct ctcatttgcc gagattgccg acggtaccgg tctggcagaa gccttcgtaa
    11821 ccgcggcttt gctggggcag caggcgcttc ctgccgacgc cgcccgcctg gtcggggcga
    11881 agctggatct cgacgaagac gccattctac tgttgcagat gattccactg cgtggctgca
    11941 ttgatgaccg tattccaacc gacccaacga tgtatcgttt ctatgaaatg ttgcaggtgt
    12001 acggcacaac cctgaaagcg ttggttcatg agaagtttgg cgatggcatt atcagcgcga
    12061 ttaacttcaa actcgacgtt aagaaagtgg cggacccgga aggtggcgaa cgtgcggtca
    12121 ttaccttaga tggtaaatat ctgccgacca agccgttctg acagccatgc gcaatcatca
    12181 aaagacgttc acgatgctgc tggttctggt gctgattggt cttaatatgc gaccactgct
    12241 cacctccgtc ggtccactgc taccgcaatt gcgccaggcg agcggaatga gctttagcgt
    12301 ggctgccctg ttgaccgctc tgccggtggt caccatgggc gggctggcgc tggccggaag
    12361 ctggcttcat cagcatgtca gcgaacgtcg cagcgtcgcc atcagtctgt tgctgattgc
    12421 cgtcggtgca ttgatgcgtg agctttaccc ccaaagtgtg ctgctgctta gcagcgcact
    12481 gcttggtggg gtggggatcg gtatcattca ggcggtgatg ccttcagtga ttaaacggcg
    12541 gtttcagcaa cgcacgccac tggtgatggg gctgtggtct gcggctctga tgggcggcgg
    12601 tggacttggt gccgccataa cgccctggtt agttcaacat agcgaaacct ggtatcaaac
    12661 actcgcctgg tgggcgctgc ctgccgttgt tgcgctcttt gcctggtggt ggcaaagcgc
    12721 ccgcgaggtc gcctcttccc acaagacaac aaccactccg gttcgcgtgg tattcactcc
    12781 ccgcgcgtgg acgctgggtg tttacttcgg tctgattaac ggcggttacg ccagcctgat
    12841 tgcctggtta cccgctttct atattgagat tggtgccagc gcgcagtaca gcggttcctt
    12901 actggcattg atgacgcttg ggcaagccgc aggagctttg ctgatgcctg ctatggctcg
    12961 ccatcaggat cggcgcaaac tgttaatgct ggcgctggtg ttacaactgg tggggttctg
    13021 cggctttatc tggttgccgc tgcaattgcc ggtattgtgg gcgatggtgt gtgggttagg
    13081 tctgggcggc gcgtttccgc tctgtttgct gctggcgctc gatcactctg tgcaaccggc
    13141 tattgctggc aagctggtgg cgtttatgca gggaatcggt tttatcatcg ccgggcttgc
    13201 cccgtggttt tctggcgtgc tgcgtagtat cagcggcaat tacctgatgg actgggcatt
    13261 tcatgcgctg tgcgtcgttg ggctgatgat cataaccctg cgttttgcac cagtacgttt
    13321 tccgcagctg tgggtcaaag aggcatgatg cgacgcttgt tcctgcgctt tgttcatgcc
    13381 ggatgcggct aatgtagatc gctgaacttg taggcctgat aagcgcagcg tatcaggcaa
    13441 tttttataat ttaaactgac gattcaactt tataa
//
LOCUS       DAZFAH010000120        13450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFAH010000120 DAZFAH010000000
VERSION     DAZFAH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.120"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..310
                     /locus_tag="ACHJWB_003238"
     CDS             <1..310
                     /locus_tag="ACHJWB_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4314817.1"
                     /translation="SPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPINKAYTPYN
                     IHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF
                     "
     gene            634..2022
                     /gene="narU"
                     /locus_tag="ACHJWB_003239"
     CDS             634..2022
                     /gene="narU"
                     /locus_tag="ACHJWB_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4314818.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2104..5844
                     /gene="narZ"
                     /locus_tag="ACHJWB_003240"
     CDS             2104..5844
                     /gene="narZ"
                     /locus_tag="ACHJWB_003240"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4314819.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETGLSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PTELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5841..7385
                     /gene="narH"
                     /locus_tag="ACHJWB_003241"
     CDS             5841..7385
                     /gene="narH"
                     /locus_tag="ACHJWB_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4314820.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7385..8080
                     /gene="narW"
                     /locus_tag="ACHJWB_003242"
     CDS             7385..8080
                     /gene="narW"
                     /locus_tag="ACHJWB_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4314821.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDAMALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8077..8757
                     /gene="narI"
                     /locus_tag="ACHJWB_003243"
     CDS             8077..8757
                     /gene="narI"
                     /locus_tag="ACHJWB_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4314822.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8837..9730
                     /gene="yddE"
                     /locus_tag="ACHJWB_003244"
     CDS             8837..9730
                     /gene="yddE"
                     /locus_tag="ACHJWB_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4314823.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGY
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9826..10671)
                     /gene="nhoA"
                     /locus_tag="ACHJWB_003245"
     CDS             complement(9826..10671)
                     /gene="nhoA"
                     /locus_tag="ACHJWB_003245"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO4314824.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10846..11415
                     /gene="yddH"
                     /locus_tag="ACHJWB_003246"
     CDS             10846..11415
                     /gene="yddH"
                     /locus_tag="ACHJWB_003246"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4314825.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11419..11562)
                     /gene="pptA"
                     /locus_tag="ACHJWB_003247"
                     /pseudo
     CDS             complement(11419..11562)
                     /gene="pptA"
                     /locus_tag="ACHJWB_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
     gene            complement(11710..13032)
                     /gene="ltrA"
                     /locus_tag="ACHJWB_003248"
     CDS             complement(11710..13032)
                     /gene="ltrA"
                     /locus_tag="ACHJWB_003248"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO4314826.1"
                     /translation="MNVERRGSVGQSASQPNCKQEEAAGEQTKPFQVSKLHVVEAYRR
                     VKANAGAAGVDNQTLKDFERDLKGNLYKIWNRLSSGSWMPPPVRAVEIPKKDGSKRLL
                     GIPTVSDRIAQMTVLVTFEPLVERYFLNDSYGYRHGKSALDAIAVTRKRCWQYDWYLE
                     FDIKGLFDNIPHDLLLRAVDKHCADKWVRLSIRRWLTAPVQMPDGTLKERNKGTPQGG
                     VISPVLANLFLHYVFDKWLSLHYPEIPWCRYADDGLIHCGSKQQAEELLNKLAKPFQE
                     CGLELHPEKTKIVYCKDSERQANHETVQFNFLGYTFRARRARNQRRGNLFTSFSLAVS
                     NSAQKDMIGKLRKLRLRRRVEMSLEDIAKRLNPMISGWLNYYAKYYKSAMKKVCRYIN
                     LTLIAWARKKYKTLRYKKTKACQLMERLSKEKPELFAHWKAGPGSAFA"
BASE COUNT         3173 a         3547 c         3382 g         3348 t
ORIGIN      
        1 ttccccagca cttaagctta atactggcct ggcaattctt tcgcctggtg catttgaagt
       61 tcactctgac ggaatagatg tggataacga attgtttcac tatcctatta ataaagcata
      121 taccccatat aatatacaca cttacaagac agaggaagtt gtaaaccaga ggaatataaa
      181 agttaaaaac atgaccttag atgaaataaa caatacttac tgtaataacg attattacaa
      241 tcaggcaata agagaggaac cgatagacct tctggacaga tcgttttcct ccagttcatg
      301 gcctttttag tgaataaatc atcactaatt ctataagtgt ctggtcttgt tgataatata
      361 gttaatatgt aaccctttag attcacgatc tgaagggttg aaacatgaca tacgttcaaa
      421 gattaacccc cgtttgaagg ttagcttgtt agttacattt agtaacacat atctggctac
      481 ttaatcccca ttctgccccc gcggttacgc tttcttatct attctttcaa tcagcacttc
      541 acccaattca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      601 tttcattacc aatgtgtgca tgtgaggaac aatatggcac tgcaaaatga gaaaaatagt
      661 cgttatcttt tacgcgactg gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa
      721 catattgctc gaagaaacct ctggatatca gtcagttgtc tacttcttgc cttctgtgtc
      781 tggatgctat ttagcgcagt taccgttaat ctcaataaaa tcggttttaa ttttactacc
      841 gatcaactct ttttattaac cgcattaccc tccgtttctg gcgcattatt gcgtgttccc
      901 tactccttta tggtgcctat attcggtgga cgccgatgga cggtttttag tactgcaatc
      961 ctgattattc cttgcgtctg gctcggaatt gccgtgcaaa atccgaatac tccttttggg
     1021 atatttatcg ttatcgcttt gctatgcggt tttgcaggtg caaactttgc ttcgagcatg
     1081 ggcaatatca gtttcttctt tccaaaagcc aaacaaggga gcgctcttgg gattaatggc
     1141 ggattaggaa acttaggtgt aagtgtaatg cagctggttg caccgctggt catttttgta
     1201 cctgtatttg cctttctcgg cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg
     1261 ctggcgaatg ccgcatggat ttgggtaccg ctactggcga ttgccacgat cgccgcctgg
     1321 tcagggatga atgatatcgc cagttcacgc gcctcaattg ccgaccagct ccctgtctta
     1381 caacgcctgc atctctggct gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc
     1441 ggtttttctg cgggttttgc catgctggca aaaacccagt tcccggatgt gaatattctg
     1501 cgcctggcgt tctttggccc atttatcggt gccatcgcgc ggtcggttgg tggtgctatt
     1561 tccgataagt tcggcggcgt gcgggtgacg ttgatcaact ttatttttat ggcgattttc
     1621 agtgccctgc tgttccttac cttaccgggc acaggctccg gtaatttcat cgccttttac
     1681 gccgtattta tggggctgtt tctgactgcg ggtctgggaa gtggttctac tttccagatg
     1741 atcgccgtca tctttcgcca gataaccatt tatcgggtaa agatgaaagg cggtagtgat
     1801 gagcaagctc agaaagaagc cgtcaccgaa acggcggcgg ctctgggctt tatctcagcc
     1861 attggcgcag tgggcggctt ttttattccg caggcgtttg gcatgtcgct caatatgacc
     1921 ggctctccgg tcggcgcgat gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg
     1981 acctggctgg tttatggtcg gcggaagttc agccaaaaat aagagatcga tgcctgatgt
     2041 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagtcat
     2101 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     2161 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     2221 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     2281 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     2341 cgcactcgcc ctgacctgcc caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     2401 tcctggtatc tttacagcgc taaccgcctg aaatacccgc tcattcgtaa acgactgatt
     2461 gaactgtggc gcgaagccct caaacaacac agcgatccgg tactggcgtg ggcatcgatt
     2521 atgaacgatc cgcaaaagtg cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc
     2581 cgctccaact ggcaggaact aaaccagctg attgccgccg ctaacgtctg gaccatcaaa
     2641 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     2701 tacgccgccg gaacgcgtta tctgtcactg cttggcggca cctgtttaag tttctacgac
     2761 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     2821 gaatctgccg actggtataa ctccagctac atcatcgcct gggggtctaa cgtaccgcag
     2881 acacgtacgc cggacgccca cttctttacc gaagtacgct acaaaggcac taaaaccatc
     2941 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     3001 caaggcactg acagcgccct ggcgatggca atgggccatg tgattttaaa agagtttcat
     3061 ctcgataatc ccagcgacta ctttatcaac tactgccgcc gctacagcga catgccgatg
     3121 ctggtaatgc tagagcctcg cgacgatggt agctacgttc ccgggcgcat gatccgcgca
     3181 tctgacctgg tggatggact gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt
     3241 aataccgcag gtgaattggt agtgccgaac ggttcgattg gtttccgctg gggagaaaaa
     3301 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccggattgtc gttaaccctg
     3361 ctcggtcaac atgacgctgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     3421 ccgcattttc gcagcgtaaa acacaatccg gtgctggtgc gccaattgcc cgttaaaaac
     3481 ctgacgttag tcgatggcaa cacctgtccg gtggtcagcg tttatgattt ggtactggcg
     3541 aattacggcc tcgatcgcgg gctggaagat gaaaacagtg cgaaagatta cgctgaaatc
     3601 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     3661 accatcgctc gtgaatttgc cgatactgcc cataaaacgc atgggcgctc aatgattatc
     3721 ctcggcgctg gcgtcaacca ctggtatcac atggatatga actaccgtgg gatgatcaat
     3781 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc
     3841 caggaaaaac tgcgcccaca aaccggctgg ttgccactgg cctttgcgct cgactggaac
     3901 cgaccaccgc gccaaatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     3961 tatgaaaaag tctctgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     4021 ggtcatctga ttgatttcaa cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg
     4081 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc
     4141 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gtatggcctg cgaacaacca
     4201 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gctctaacct gcttggctcc
     4261 tccggcaaag gccacgagta tatgcagaag tatctgctgg ggaccgaaag cgggattcag
     4321 ggcgaggaac tcggtgccag cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg
     4381 gcgattgaag gcaagctcga cctgctggtg acgctcgact tccgcatgtc cagtacctgc
     4441 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaacacc
     4501 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     4561 cgcagcgact gggaaatcta caaaggtatc gccaaagcat tttcgcaagt gtgcgtgggt
     4621 catcttggca aagaaaccga cgtggtatta caacccctac tgcatgactc tccgacagag
     4681 ctctcacagc cgtgtgaagt gctcgactgg cgcaaaggcg aatgcgatct gattccaggc
     4741 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctacgta tgaacgcttt
     4801 acctcgctcg ggccattgat ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat
     4861 acccaggatg aaatcgattt cctcggcaaa ctcaattaca ccaagcgtga tggcccggca
     4921 caggggcgtc cgctgattga caccgccatt gacgcttcag aagtgattct ggcactggca
     4981 ccggaaacca acggtcatgt tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg
     5041 cgcgaacata cccatctggc gctgcacaaa gaggacgaga agattcgctt tcgcgatatt
     5101 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ccggtctgga aagcgatcac
     5161 gtatcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
     5221 ggacgccagc agctctatca ggatcatccg tggatgcgtg cttttggtga aagcctggtg
     5281 gcttatcgcc cgcctatcga cacccgtagc gtcagtgaga tgcgccagat accgccaaac
     5341 ggcttcccgg aaaaagcact taacttcctg acgccgcacc agaaatgggg cattcactca
     5401 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
     5461 agcgaaaccg atgcccgaga actgaccatt gtcgataacg actgggtgga agtatttaac
     5521 gccaacggcg cgctgactgc ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc
     5581 atgatgtatc acgcccagga acgcattatg aatattcctg gttcggaagt aactggcatg
     5641 cgcggcggca ttcataactc ggttacccgc gtttgcccga aaccaacgca tatgattggc
     5701 ggttacgcgc agctggcctg gggctttaac tactacggca ccgtcggatc gaaccgcgat
     5761 gagttcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
     5821 caggtacagg aggcgaaaaa atgaaaatcc gttcacaagt cggcatggtg cttaacctcg
     5881 acaaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accgggcgcg
     5941 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
     6001 aaaactggga agatcaggaa gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga
     6061 tccgtccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
     6121 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
     6181 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
     6241 tggacaaagt gatctggggc ccaaactggg aagaactgct gggcggcgag tttgaaaaac
     6301 gtgcccgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
     6361 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagt tgcgtggcga
     6421 cctgcccaag cggcgctatc tacaaacgcg aagaagacgg cattgtgctg attgatcagg
     6481 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
     6541 actggaaaag cggcaagtca gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc
     6601 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
     6661 acgacgccga ccgcattgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
     6721 gtcagtgcga agtgttcctc gatccacacg atccctcagt gatcgaggaa gccctgaaac
     6781 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gcctgtctac aaaatggcga
     6841 tggactggaa actggcgcta ccgctgcacc ctgaatatcg caccctgcca atggtctggt
     6901 acgttcctcc gctgtcgccg attcagtcct acgcagatgc gggcggtttg ccgaaaagcg
     6961 aaggcgtgct gcccgccatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
     7021 tgagtgccgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
     7081 actatatgcg ttcacaaacc gtggaaggcg ttactgatac tcgtgccatc gacgaagtag
     7141 gcctgagcgt cgcccaggtc gaagaaatgt atcgctatct cgccattgcc aactatgaag
     7201 atcgttttgt tatcccgacc agccatcggg aaatggcggg cgatgccttc gcagaacgca
     7261 acggctgcgg ttttaccttt ggcgacggtt gtcacggctc ggacagcaaa ttcaacctgt
     7321 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
     7381 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggatg agctgttgtg
     7441 ggaatgcaag gaggacgcga tggcgttgat ccgccgcgac gcgccgatgc ttacggattt
     7501 cacccacaac ctgcttaacg cgccgctgct ggataaacag gccgaatggt gcgaagtgtt
     7561 tgaccgcggg cgcaccacgt cgctgctgct gttcgaacat gttcatgccg agtcccgcga
     7621 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggcc tgcagctgga
     7681 ttgtcgggaa ctgcccgatt atctaccgct gtatctggag tatttaagcg tactgcctga
     7741 cgatcaggcg aaagaaggat tgctcaatgt tgcgccgatc ctcgccctgc ttggcggtcg
     7801 cttaaaacaa cgcgaggcac cgtggtacgc gttgtttgat gctctgctgc aactggcggg
     7861 aagtatactt tcaagtgaca gtgtcacaaa acaagtaaac agcgaagagc gcgatgacac
     7921 ccgccaggcg cttgatgcag tgtgggaaga ggaacaggtg aagtttattg aagataacgc
     7981 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
     8041 gccgcagtat gtcgacatca gtgcgggagg tgagaaatga ttcagtatct gaacgtcttt
     8101 ttttacgata tctacccgta catttgtgcg acggtgtttt tcctcggcag ctggctgcgc
     8161 tacgactacg ggcagtacac ctggcgcgcc tcctcaagtc aaatgctcga taaacgcggg
     8221 atggttatat ggtcgaatct gttccatatc ggcattttgg ggatcttctt cgggcacctg
     8281 ttcggcatgt taacgccgca ctggatgtac gcgtggtttt tgccagtggc agcgaaacaa
     8341 ctcatggcga tggtgctcgg cggtatttgc ggtgtgttga cattgattgg cggcgcagga
     8401 ctgctgtggc gcaggctgac aaatcagcgc gtgcgtgcaa cttccactac gccggatatt
     8461 atcatcatga gcattttgct gatccagtgt ctgctggggc taagtaccat accgttttca
     8521 gcacagtatc ctgatggtag cgaaatgatg aagctggtgg gttgggcgca aagcattgtc
     8581 actttccgtg gtggatcgtc agaaatgctt aacggcgtag cgttcgtttt ccgcctgcat
     8641 ctggtattgg gaatgaccat cttcctgctc ttcccgttca ccagattggt gcatgtgtgg
     8701 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgataattt
     8761 tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
     8821 ccacaggaga acgttaatga aaccgcaggt gtaccacgtc gatgctttta cctcacaacc
     8881 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg aagcgcaaat
     8941 gcagcttatc gcccgcgagt taggctattc ggaaactgct tttctgctgc acagcgacga
     9001 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gtgccgattt gcggtcacgc
     9061 gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt gcacgatctg
     9121 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
     9181 tatttcgctg gaacaaggta cgccgggctt tgagccaccg ctggaaggtg aaacacgtga
     9241 ggcgattatc aacgcgcttc atctcactga agacgatatt ttgccgggct tgccaattca
     9301 ggtcgcaaca acaggacact caaaagtgat gatcccgttg aaaccggaag tggatatcga
     9361 cgccctttcg cctgatcttg ctgcgctgag cgctatcagc aaacagattg gctgcaatgg
     9421 tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc gatggtcgta tgttctcgcc
     9481 tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
     9541 gttggtacat cacaacgtat tgccccacga tggcaacgtg ttatgtgtta aaggccatca
     9601 ggggcgcgca ttggggcgtg acggcatgat tgaagtgacg gtgacaattc gtgataacca
     9661 accggaaaaa gtcaccattt caggcacagc ggtgattttg tttcatgccg aatgggcaat
     9721 tgaactctga aaccgttgcc tgatgcgctt cgcctgtcag gtctacaacc gagctaatgt
     9781 atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt ttcccgcctc
     9841 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacggtaa agccatgttt
     9901 cgcatcatcc acgcccagac caaactgttc ttgcatcaca gcatataatg acgccacatc
     9961 cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggtaaaat gaaaattggt
    10021 cagcgttagc ttgccaccgt ccggcaaatg gcggcacatc agcaaatgat ggcgaaaatg
    10081 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    10141 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    10201 atcatcaccc tcctgcaaca accgataatc tccgtgtggc gtggtctgca cgagatcgga
    10261 aactaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    10321 ttcctcttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    10381 tgataacact acgcgcccta acaagctgcg aacgttaaac tccagctcgc gtaacacccg
    10441 ctcaaacacg ccattctgct caaaacagta accgccacga cgggctatca ccagtttctc
    10501 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    10561 cggaatagtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tgaccgcagc
    10621 agctcccgac cagttaatac gggcaaaata gtgattcaga atgggcgtca tcaggcgttt
    10681 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcactggct attttgtgcg
    10741 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtc gcttattgcg
    10801 ttacgaacta cgcttttaaa acaaccttct tagcgaggcg ataccgtgag ccgattcatc
    10861 cccgtagaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    10921 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    10981 gagtttgaac cgccacgcgt ggcgattgtg gtagataaat cgacatggac cagggagttg
    11041 attgaacgta acggtaaatt tggcatcgtt atcccgggcg ttgcggcgac taactggacg
    11101 tgggcggtgg gaagcgtgtc gggtcgtgaa gaagataaat ttaattgcta cggcattccg
    11161 gtagtgaaag gcccggtgct tggtttgcct ctggtcgagg aaaaatgtct ggcgtggatg
    11221 gagtgccgcc tgctacctgc gacttctgcg caacaaaaat acgacacgct gtttggcgag
    11281 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    11341 gataagctca atacgttgca tcatttaggt gctggcacgt ttgttactag cggcaagcgt
    11401 gttacggcag gttaagcatt acatgctgta accgggtttc tttatcaaag cctccatttg
    11461 gggctcgatt tcggcatccc agatagcttg ccaagattct ggttgaatct gctgtagagc
    11521 aatgcttatc gagctatctt tactgttcag atggcgaata atttaggtaa tgaggaatat
    11581 ctcaattttt aaacataaaa tgcgaattat ttagtacaaa aagtgtactt ctagtgagta
    11641 ggccaccgga acctccccga cagcctctcg cagaaccgta cgtgaaactc tcgcttcata
    11701 cggctcctgt caggcaaacg cgcttcctgg tcctgctttc cagtgggcaa atagctccgg
    11761 cttctctttc gacagtcttt ccattaactg acacgccttc gtctttttat accgcagggt
    11821 cttgtatttc tttctcgccc acgcaatcag cgtcagatta atatatctgc atactttctt
    11881 catcgctgac ttgtagtatt tcgcgtaata attcagccac cccgatatca tcggattcag
    11941 ccttttcgca atatcttcaa gactcatttc caccctgcgt ctgagccgca gtttcctgag
    12001 ttttccgatc atgtctttct gcgcactgtt actcaccgcc agactgaaac tcgtaaagag
    12061 atttcccctt cgctgattac gtgctcttcg cgctctgaac gtatacccca gaaaattaaa
    12121 ctgcactgtt tcgtggttcg cctgtcgttc gctgtctttg cagtaaacta ttttcgtttt
    12181 ctccggatgt aattccagtc cacattcctg aaaaggtttt gccagtttat tcagtaattc
    12241 ctctgcttgt tgtttactgc cacaatgaat taatccatca tctgcataac gacaccaggg
    12301 aatttccgga taatgtaacg acagccattt atcaaacaca tagtgcagaa ataaattcgc
    12361 cagtaccgga ctgatgactc caccctgtgg cgtaccttta tttctttcct ttagtgttcc
    12421 atccggcatc tgcaccggcg ctgtcagcca cctgcgaata gacagtctta cccatttatc
    12481 cgcacaatgt ttatccactg ccctgagtaa taaatcgtgg gggatattat cgaataaacc
    12541 tttaatatca aactcaagat accagtcgta ttgccagcaa cgttttctgg tgactgctat
    12601 tgcatccagc gctgattttc catgccggta tccgtatgaa tcattcagga aataacgctc
    12661 cactaatggt tcaaacgtta ccagcaccgt catctgcgct attcgatcgc ttactgtcgg
    12721 tattcctaaa agtcttttac tgccatcctt cttcggaatt tccaccgcac gcactggcgg
    12781 cggcatccag cttcccgacg ataaccggtt ccagattttg tacagatttc ctttcagatc
    12841 ccgctcaaaa tctttcagcg tctggttatc cactccggca gctccggcat tcgcctttac
    12901 ccgccggtac gcttccacca catgcaattt actgacctga aacggttttg tctgttcacc
    12961 cgcagcctcc tcctgtttac agttgggctg tgatgcagac tgcccgacgc tgccccttcg
    13021 ctccacgttc attacaaacg cttcctcact actacgaaca gctctgcccc ggtttcgcac
    13081 atcgatactc tcatactcat aatttcggtt atttgtattt ctctcttgac attgcgacga
    13141 ccggttcccg tagttcctgc aaagagcctg atacagcgtc atgcctgcta tacgccggac
    13201 accattcacg cagcattcgg aattcacccg taaatttatc ccggcggtat catcatgcct
    13261 ccggttttga tgcctcttag gactaacgac gtttcaacac aggttcgggt ttactcatct
    13321 cactgtatct cacacaggat cagatcgcgt ctgatcgttc cccctgcact cactaccatg
    13381 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
    13441 gcctgccacc
//
LOCUS       DAZFAH010000121        13387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFAH010000121 DAZFAH010000000
VERSION     DAZFAH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.121"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..884)
                     /locus_tag="ACHJWB_003249"
     CDS             complement(18..884)
                     /locus_tag="ACHJWB_003249"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFO4314827.1"
                     /translation="MASNYGENFTPLFKRFIKYIFKNPIAKTTTNKERFHLNLLEMTI
                     YGEMASFNAAGCEIFISEQRLADDYDVSRPTVINSIKSLENAGLISIVKSKGKSNHYK
                     LLITPDQLGKMCDEQGIKTSDSVGSSKEAPDNQQEAPKQATQQAKKAEVVQLPTQPKN
                     ERRERFRDAGKREKDEHGYPILTSDEMNAKYKGIQATFKDRKSIKLSELRDAFYGPEV
                     MDKIKGTVEGRIWKEDDYWHLTEQALKVAQHVAKRGKTIIIDYISHGYDAPKERLWNN
                     CKLVKGELTITK"
     gene            complement(898..1113)
                     /locus_tag="ACHJWB_003250"
     CDS             complement(898..1113)
                     /locus_tag="ACHJWB_003250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314828.1"
                     /translation="MAKTGPISQLVNWTTDEIVENVLKGNFRKTSVYAFIRKHKGEYP
                     HIEEAILKLNCIRLERELAKYTNNINK"
     gene            complement(1247..1396)
                     /locus_tag="ACHJWB_003251"
     CDS             complement(1247..1396)
                     /locus_tag="ACHJWB_003251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314829.1"
                     /translation="MEITDAIAIINLAVLWCGVAYIAGVTAIDNLEEKMIDKYKKIVD
                     NLTNV"
     gene            complement(3788..4309)
                     /locus_tag="ACHJWB_003252"
     CDS             complement(3788..4309)
                     /locus_tag="ACHJWB_003252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314830.1"
                     /translation="MLFQKDKESVNKRLFKELVKMFFQYTAAFLIAGPWLMAICFYPA
                     TLFIGSSKFGDGPFHVDIGFIDSLLIRPINAVLNAFYFPTSLEGWAVTWGVLGLAYVY
                     WLVGFLWGAICALLDIDTGSSRRSGGNNSGAGVYAHVGTRGVSITAGTRFAGGWLSIG
                     KRGVSWRKSKKLF"
     gene            complement(4367..4627)
                     /locus_tag="ACHJWB_003253"
     CDS             complement(4367..4627)
                     /locus_tag="ACHJWB_003253"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4314831.1"
                     /translation="MTGAKDIRRLREELIAEIKAKAKQMEIAAQMDVAEQMYKMVEEG
                     TPRQVIAKTFGVGIATVHRRLKAYADYHNIERGEDGRFKKNK"
     gene            5668..6003
                     /locus_tag="ACHJWB_003254"
     CDS             5668..6003
                     /locus_tag="ACHJWB_003254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314832.1"
                     /translation="MRDMTIRTRGHIISYAADEQFSIRDVEIKNKVKAEIPAGTVVVV
                     TEEGEVETFVKATHTGKTPLLVVERAEVGDAFIVVANKGCYINPAYVDKEGLAAVIAA
                     SNGDLRVAQ"
     gene            6015..7058
                     /locus_tag="ACHJWB_003255"
     CDS             6015..7058
                     /locus_tag="ACHJWB_003255"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4314833.1"
                     /translation="MKKEFEVRDLCGLIKDIPLDNQIISAMDIFNTTALCNTFKFEYD
                     KVRQVKQTAFGQYPRYGEQMATITADERETGTGDMAFTAASSAIYPSDWQGIRVPGKD
                     SEENLESVTARRVIQLGEGLRQTHEEARQLALFENKIILTKGKELNVCELLGVEQSTS
                     EMDLAQDILKQLQDLVALSKVKQRGVVRALRGFTLFCRAEVFAAIQTDELLRKVLLFG
                     NQNITDKGLMFPEDILPGFPTFRIPGLALRVVQMDSLPENTAYLVPQFQPHALGDMVG
                     VYTQFFGPCARNLKLANGAAREAFMYMEQSRGKREIFIESSYAFMNMMPEVVVKVSVS
                     NSAAAALAKAKAK"
     gene            7172..7423
                     /locus_tag="ACHJWB_003256"
     CDS             7172..7423
                     /locus_tag="ACHJWB_003256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314834.1"
                     /translation="MKIVLYGDRDDVPFDIIHIDDEDLPEFLRISTFAACYDVSRQAM
                     HKYREYNKCTWAETFEHFIARKQAQDRKKALNAHNRFAK"
     gene            7583..8386
                     /locus_tag="ACHJWB_003257"
     CDS             7583..8386
                     /locus_tag="ACHJWB_003257"
                     /inference="COORDINATES: protein motif:HMM:NF044880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO4314835.1"
                     /translation="MANDALTAAKQAESRSPAFTVMCGGYSQNKNIMDGFNYTDNVAI
                     FFDSISDVRYSFPVEKSSYAVEDGTTMSDHAVIKDKSVSFTGIITSSPHIIRKENYID
                     RNTDPDNPRESMRPAAAVRALMEMRDKRQLVSLVSEDAGLLENFIMTKCEVKRSSSSG
                     DCITIDCTFEEFRTFKVKMVENAGVFTNSKKVNKTQQKGAVNSAKEGSNAGNAATKST
                     RAKHRGEYAQGEAAGKIAAGDKSVVSNEFHETMIDKANMLLDDITEINK"
     gene            8383..8841
                     /locus_tag="ACHJWB_003258"
     CDS             8383..8841
                     /locus_tag="ACHJWB_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016068527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314836.1"
                     /translation="MSPKVRCTIDKRMMNKLHKELSQMEGVAVGWDDGKHYSGLNYAT
                     LAAIHSEGWGDLPKRSFMESAHAAMLYESLRHTKALINAIVKGGSSEAVRKRIGKRYA
                     DILETILISGQFSNNKVSPQWASYKGFNDAMRHYDDLIGAIKYWSNVGRG"
     gene            complement(9358..11460)
                     /locus_tag="ACHJWB_003259"
     CDS             complement(9358..11460)
                     /locus_tag="ACHJWB_003259"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4314837.1"
                     /translation="MAMKEVAKLTNVIYWDSDKSSWRKEVKRLEDYKKSWKKATDDIA
                     KADFKSNQAVLRTQRTRAKVEEQIARAQKKQRAQAVSRLTGGNNPELSSMRKFYQQQA
                     REAARQQKLLDRSVGDVQHRKARVGANSARISSMFDGSLVAQFQSRIQRVNADYAKGA
                     INLQTYNQKMARLRAEMKMATAEAGKFQSRLDRAAIAGSKLHDNLGKLNSTLGKIAIG
                     LTAAGWGVQRAVSGAMGSLADRNSAYFGLMAQNKDDKAKTDQQMAYVKRISYDYGMDQ
                     RETEGGFMRFAAATPSIKEKDKQDLFEAMAIKGRSVGATADQQNRAIVALGQIYSKGG
                     RVYAEELKGQLSEALAGSQVDFTRAAIDAGIIKKEEEFNKALEDGKITMDKLLPSLVK
                     IWGAAKETKAFAESMKQPEVAMQRMKNSFNELAIQFMGVVDPADGVISLSESLISIFE
                     DLTTDMDGSGSAAKQFGEAVGAAMREIMYWVGFAKGYIEVKAKEFGMTGSEVGELIGK
                     LVVGLAGLKVITTLLSPLTGFVSLVTQLIALLGKIPGMSKLLASASGGTATGAAVISA
                     GSNAERSFIDGKTQEMSKENSKQGALFELGRFVSNAGALFTPGGDTGPLFDQVRGWFN
                     KGPTLTVDTGIRPTPTGGMAIQNGGYQPVQVNVAPQGEISGKWEITIDEGGIARVIQK
                     EVSDQLDWKATQESNQLF"
     gene            complement(11464..11649)
                     /locus_tag="ACHJWB_003260"
     CDS             complement(11464..11649)
                     /locus_tag="ACHJWB_003260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314838.1"
                     /translation="MNTYEKIYQKLKSDNNAPVIKGGASSSGDLSIEELAEKYNIDKE
                     ALKRAISLSKSLRQGEV"
     gene            complement(11651..12034)
                     /locus_tag="ACHJWB_003261"
     CDS             complement(11651..12034)
                     /locus_tag="ACHJWB_003261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314839.1"
                     /translation="MNIEEMIVEFDDGDFLEDGEFKYSRFKQALFSSDDKPHVSDLND
                     DDLCRWVCTRLYEDGKYTGQNFPNLYFKAQTFEEGQRVKNLVDKMLSAGYLHKELLNP
                     KAESKRHYIFVWTIGDKCPLRRKGK"
     gene            complement(12113..12556)
                     /locus_tag="ACHJWB_003262"
     CDS             complement(12113..12556)
                     /locus_tag="ACHJWB_003262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314840.1"
                     /translation="MSQIAKLLSQVIKAFDAEDSKVRQDLLDGKFTNTLAFTRDKEHF
                     LKHRHHAFAECKSANGDVFYVDLVVMACRLWMIQDLMRQHGDDPLISMWNSAQDGFVI
                     AALSKSMIEAEGASLQNMADLFEISVGMMQTMLQAVKPLKLVNLE"
     gene            complement(12630..13118)
                     /locus_tag="ACHJWB_003263"
     CDS             complement(12630..13118)
                     /locus_tag="ACHJWB_003263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314841.1"
                     /translation="MFKSDLSDSQFEKLVALQKYLPSDDQPTRQAKHLLVEGFTPEFV
                     AEYTGVKLEKVQKLYNESWNPRCRVPLSVYNEIDRDRNVFEDRRRKSLLMWNCGMNIE
                     EIAKELKVTCFHIWGLLSEILPRKELNQRLPKPGSRAYREFIFIYRRHSNKVRKAMAQ
                     KA"
     gene            complement(13217..13363)
                     /locus_tag="ACHJWB_003264"
     CDS             complement(13217..13363)
                     /locus_tag="ACHJWB_003264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314842.1"
                     /translation="MYGDFDYYLEKEKYFEDELSQHFSYWEEYCGWLAEDPADEENDS
                     DIPY"
BASE COUNT         3546 a         3033 c         2899 g         3909 t
ORIGIN      
        1 aaaaaaaata agaatgttta ttttgtaatg gttaattcgc cttttacaag cttgcagttg
       61 ttccacaatc tttctttcgg ggcatcatag ccatgactaa tataatcgat gataattgtt
      121 ttacctcttt tagctacatg ttgagccacc ttaagggcct gttctgttaa atgccaataa
      181 tcatcttctt tccagattct accctcaacg gtgcctttaa ttttatccat cacctccggc
      241 ccgtaaaatg catctcgcaa ttcagagagt ttaatgctct tccgatcctt aaatgttgcc
      301 tgaatccctt tgtatttggc gttcatctca tcacttgtca gaatcggata accgtgctca
      361 tctttctcac gtttgccagc gtctctaaag cgctctctgc gctcgttctt aggttgggta
      421 ggcaattgca ccacctcggc ctttttagct tgctgtgtgg cttgttttgg ggcttcctgt
      481 tgattatcgg gggcctcttt actagatccc acactgtcgg aggttttaat accctgctca
      541 tcacacatct tgccaagctg atcaggggta attagcagtt tgtaatgatt agatttgcct
      601 ttacttttaa ctattgaaat aagcccggca ttttctagag atttaatcga gttaataact
      661 gtcggtcttg aaacatcata atcgtctgct aatctctgtt cagagataaa tatttcacaa
      721 cctgcagcat taaaacttgc catctcaccg taaattgtca tttccaacaa gtttaagtga
      781 aatctttctt tattggttgt agtttttgca atcggatttt taaaaatgta tttgatgaaa
      841 cgtttaaaaa ggggagtaaa attttccccg taattacttg ccataattcc cccataatta
      901 tttattaatg ttattggtgt atttagccag ttcccgctca aggcgaatgc aatttagttt
      961 taaaattgct tcttcaatgt gtggatattc tcctttatgt ttacgaatga atgcataaac
     1021 ggaagttttg cggaagttcc ccttaagtac gttctcgacg atttcgtcag tagtccagtt
     1081 taccagctgg ctgatcggtc cggttttagc catagtataa gcctcctgtt agctcactaa
     1141 cctgtgcgat atatgattaa aaagaaacgg gaacttaaga caagcccccg ccagtctact
     1201 aaaggaaatt cactatgatg agaattaccc tatgctttat attttatcag acattcgtca
     1261 aattgtcaac aatctttttg tatttatcta tcattttttc ttcaagattg tcgattgcag
     1321 ttactccggc tatgtaggca acaccgcacc acagtacagc caggttgatg atcgctattg
     1381 catcagtaat ttccacattg acctccggat ctgtttctcc ccctatttat aatcttatca
     1441 tattgtcaac caattgtcaa tgagaattat tatcatttag gtgtgctata ggtgcaagca
     1501 agtccacagc aaaggggtaa acattattaa tggcccctac cgtgtcaaaa ccagaatcac
     1561 ttatagcatt tatgcaataa gattgtcaat agaccgatct gcaattttta tcttgatagt
     1621 taaattctct atgagaggcc ctacaacgcg atttaagcat caggtaatgc aattgcacta
     1681 ctcacacagg ttaatgcagc cagaaggcca tttaggggcc tagaattgga tttatgaatg
     1741 tatatcttac gtatacgccc acacatgagc gcgggagact ctttgctgcc tccttccggc
     1801 gctggcaagc tttcggcagc ggtccacatg tgggcctcat gctgacgcat tcgccggacc
     1861 acacacaatt gggtgacgtg tcatgtatgg aatacattgt caccccacac acaggccgac
     1921 gtagtcggtt tagctgtata gctaaagtca cagagtgacc cacacacaga ttgcctaaag
     1981 agtcgctaat gctctttagg ctttcatttg tgggttaagc ttttccctta gaaaaccttc
     2041 cccactctct gatccctccc cattccccct gggggttagg ggggatctat tgtgtggtct
     2101 attactttat ctattagtgt caaatatttt tacaggtagt agtaaaagat tttgacaggt
     2161 agtagtaaaa atattttaca ggtacgggcc gtattattgg tgaggtagta aaatattttg
     2221 acaggttagg ggctgtgtat catgggtgca atgactgcaa aacaggagag tatgactgta
     2281 taaacacaca ggtgtgaaaa ttctttacag gtagtgcgga gaaattctcc ggggtagtag
     2341 ggaaatattt accggggtgt ggtgcctgag tatgactgaa aggagttgta ggaaatccca
     2401 acaggttatt ccatcatgtg tatgtgcaag ggttgtagga aataccgaca agttatctca
     2461 tcatgtctgt gtggaaatac acccttattg acctattttg actagcaaac ctctctcctc
     2521 tctctttgct ttgatttcct ctcatgtgtg ggaaatacgc agatcaatac ccattgattt
     2581 tatcaacagc tatgttgacc gggcatcata agtatggccc ctgaggcagt ccatccttcc
     2641 ctcacacagg acaatgaata cactccttgc agtcatgaat acataaagaa catgaaagac
     2701 tgttcacacc aacacgccga taaattgcct gaagaggctt acatggggtt attgttgtgt
     2761 gggtaatggt gatcatgcta acagattatg ttaatcagat gtaaattccg attttgtggt
     2821 ctgcagagaa caaactatac tggttaaatg atcaggggaa tggttacaaa acaatcaaat
     2881 atgtgggata tagacatcta cacgtctaat ttgctgtgtg gggtgttagt gtatgcttac
     2941 gtgtaaggca ttgatttgaa gggttgggaa aattgcattt aacataatag atcttatcta
     3001 cactaataaa gcgcactaca aaatctgatt taaaaatcgt gataaggagg cccagaattt
     3061 acgtacacgc acctgaataa ataatgtgca caacataaag caaagcttaa gaattaacat
     3121 aatagcaacg tggtgggcag tggtgtgata tttgagaatt gttagaagaa aaaagggagg
     3181 tgggggagcg cctgcctggc acctcagacg aacatctgac gggtattaat accgctaatg
     3241 gatatttctg acacccacac ccaagaccgc tttcagcaca tagccataca ccgcacagga
     3301 tgcaattgtg ataacaaaaa atgggggtgt aatagggtag gtcatctttc atcaaaatca
     3361 cttctacgac gttcccagag tgataacaag aaaacaggcc tctaagatta tctgtgcctc
     3421 gtttttagct ttttgtgtgg ttaccaggtg aggaggaaat gttaaaaact gttcatttta
     3481 tgcacaaact agtcagggtg ataccacaaa ttgaggtgta agaaatccag ctgcagataa
     3541 tcttgcagcc agattttgca gttttttagg gtatgttaaa tttgacaaaa atagagaaga
     3601 ggcttgttaa gctaacttat tgattttatt agtattacga aaaagggtta caccctctct
     3661 cttactctgt caaccgggcg gttgtggcat aaaacggatt aaatcaataa ataggcatta
     3721 aatagggata aataggggtt aattgaggga taaataaaaa ggggccgtga tggccccccc
     3781 tggtaggtca gaacagtttt ttgctttttc tccaactaac gccgcgtttg cctatgctca
     3841 gccagccgcc agcgaatcgg gtacctgcgg tgatacttac ccctctggta cctacgtgtg
     3901 catatacacc cgctccgcta ttattaccac cactcctacg gcttgatcct gtatcaatat
     3961 cgaggagagc acagattgca ccccacaaaa agccaaccaa ccaatataca tatgctaagc
     4021 ctaaaacacc ccaggttacc gcccacccct ctagactagt ggggaagtag aaagcattga
     4081 gaacagcatt tataggacgg atcaggagag aatctataaa accaatatcc acatgaaacg
     4141 ggccatcgcc gaacttgcta ctgccaataa acaaggtagc agggtaaaaa cagatagcca
     4201 ttaaccaagg cccggctatc aaaaacgctg cagtgtactg aaagaacatt tttaccagtt
     4261 ctttaaaaag ccttttattt actgattctt tgtctttctg gaataacata gcggtacccc
     4321 tttgcgagaa gttgaattta gttaataacc tgttggactg ttcacactat ttattcttct
     4381 taaatctccc atcctcccct ctctcaatgt tgtggtagtc ggcataggct ttcagccttc
     4441 tgtgcacggt ggcgatcccc acaccgaagg ttttagcgat aacctgtcgt ggtgttccct
     4501 cctcaaccat cttgtacatc tgttccgcta catccatttg tgcggctatc tccatttgct
     4561 ttgccttagc tttaatttca gctatcagct cttccctcag tctcctgata tctttagccc
     4621 ccgtcataaa ctcctctcaa tgctgttcat aaactccgct caatcatgat tagtagaaca
     4681 tccaggatag ccagacctat taaaaaatgc tttgcctcct ttgcccatct cacacctcca
     4741 cacacagagc taacatcagg gctaacactc caaccacctc aattgccttg acaatgtgtg
     4801 ggtgatgaaa cctggccttt tgccatgcgc tggcactgtt cttgataccc ataactatca
     4861 ccttttgcga gaagttgatt tgtttgctcg gttgggtgct atcaatgctc cccttccttg
     4921 attcgaacta tagccgggta aatccctgga aaacattgat attgatcaaa agatatctat
     4981 caagctttat aaagtggtga tagatatgag tcgcatagct gcgaccttgg cagtacgcca
     5041 agcctacaaa gtaggtcgga tgtatgcatc taaagggtgg gggtgacgta ttgatgggat
     5101 tgatagaggt ctgaaaatac cgttaccacg ctaattatta gaatttatat aataagggtg
     5161 ttgacaattt gagagaatac tgatataata tacatatacc gtaaatcaat ggtgaaagtg
     5221 atttgccgta ttttcctcca aagtaaaggc gaccaggtag cggtgccgcc ttataaataa
     5281 cactaccctg atcatcgttg agtggtcgat taattctcct attgggctag ttatccacac
     5341 agttttcttt tgcgggtttt ctgtgtgggt agctatcccg aaaggtattt ccccgctgat
     5401 gtgaccgcag cctgaactga ttcgggggag tgcctttcgt gatagtgctc tctagtaacc
     5461 gtaacccata ctgtgaacta tcagccaact gttgttggtt tccccatgtg gaatagcccc
     5521 cgcttggacg gtgcggggct atctccctct ccttgtatca atttcccttt agatttccgg
     5581 cataactttt cggcccgagc ctgtcgcggt ttgagaggtt ataccggaac tctttgaggg
     5641 gagttaattt aaatcgcagg tgatcaaatg agagatatga ctatccgcac acgcggacac
     5701 atcatttcat acgccgcaga tgagcaattt agtattcgcg acgttgaaat taaaaacaaa
     5761 gttaaggctg agattccggc tggcactgtt gttgtggtga ctgaggaagg cgaggttgaa
     5821 acattcgtta aagcaacgca caccggtaaa actccgctgc tggttgtaga acgtgctgag
     5881 gttggtgatg cttttatcgt tgttgccaac aagggctgtt atatcaatcc ggcctacgtg
     5941 gataaagaag gcttagccgc tgttattgct gccagcaatg gcgatttgcg tgttgcacaa
     6001 taaggaggtt ggagatgaaa aaagaattcg aagtccgcga tctgtgtggc ttgattaaag
     6061 acatcccgct ggataaccag attatttcag caatggatat cttcaacact actgctctgt
     6121 gcaatacctt taaatttgaa tatgacaaag ttcgccaggt gaaacaaacc gctttcggtc
     6181 agtatccgcg ctacggtgaa cagatggcta ccattaccgc agatgagcgt gaaacgggca
     6241 cgggtgatat ggcattcact gccgcatcaa gtgctattta tccttctgac tggcaaggta
     6301 tccgtgttcc gggcaaagat tccgaagaga atttagagtc tgtaaccgct cgtcgtgtta
     6361 ttcagcttgg tgaaggctta cgccagaccc acgaagaagc acgccagctt gctctctttg
     6421 agaacaaaat tatcctcacc aagggtaaag agctgaatgt gtgcgaactg ctgggcgtgg
     6481 aacagtccac ctctgaaatg gatctagctc aagatatcct taaacagctg caggacctgg
     6541 ttgcactgag caaggttaaa cagcgtggtg tcgttcgcgc tctgcgtggt ttcaccctgt
     6601 tctgccgtgc tgaggtgttc gccgctattc agactgatga actgctccgt aaagtgctgt
     6661 tgttcggcaa tcagaatatt accgacaaag gtctgatgtt ccctgaggat attctgccgg
     6721 gcttcccgac cttccgcatt ccgggcttag ccctgcgtgt ggtgcaaatg gatagtctgc
     6781 cagagaatac tgcttatctg gtcccacagt tccagccgca tgcgttaggc gatatggtgg
     6841 gcgtttatac tcaatttttc ggcccttgcg ctcgcaacct gaagcttgca aacggcgctg
     6901 cacgtgaagc tttcatgtat atggaacagt ccagaggtaa acgagaaatc ttcattgaat
     6961 cctcttatgc tttcatgaac atgatgcctg aagttgtggt taaagtgagt gttagtaact
     7021 ccgccgccgc tgctctggca aaagccaagg ctaaataagg tccttcttcg aaggccaaat
     7081 aaaagcaaag ggaagaggtg agagatcggg gataggtctt tccctcttcc cttttttatt
     7141 atccccgtct taagtttttg aggtgtcttt tatgaaaatt gttttatacg gcgaccgtga
     7201 cgacgtgcct tttgatatta tccatatcga tgacgaggat ctgccggaat tcctgcgtat
     7261 ctccacattt gccgcctgct atgacgttag ccgccaggct atgcataaat accgtgaata
     7321 taataaatgc acctgggccg agactttcga acattttatt gcacgcaaac aagcccagga
     7381 ccgcaaaaaa gccctgaatg cccataatcg ttttgcaaaa taacgctcta gggaagagct
     7441 ataagcgatt ctaacgaata actgccctgt attttctgca gattttaagc agggcaacga
     7501 taagttctat tttgcctgca tcttttgcag gtttggcttt agccaaatcc atgaagtgga
     7561 ccaatttgga ggcatgttta atatggctaa tgatgctctg acagctgcaa aacaagctga
     7621 gtcgagatct cctgcattca ctgttatgtg tgggggatat tcacaaaaca aaaatataat
     7681 ggatggcttc aactataccg ataatgtggc tatcttcttt gactctatct ctgatgttcg
     7741 ttattcgttc cctgtcgaaa agtcgagcta tgcagtagaa gatggcacca ccatgtcaga
     7801 ccatgccgtc attaaagata aatctgtcag ctttacgggt attattacct cttcccctca
     7861 tatcatccgg aaagagaact acatcgaccg gaacactgat ccagataatc cccgtgaatc
     7921 tatgcgcccc gccgccgctg tgcgtgctct tatggaaatg agagacaagc gccagctggt
     7981 tagcctggtg agtgaagatg ccggacttct tgagaacttc atcatgacga aatgtgaagt
     8041 taagcgtagc tcttcctccg gtgactgcat cactattgat tgcacctttg aagaattccg
     8101 caccttcaag gtgaagatgg tcgaaaatgc cggagttttc actaacagta agaaggtgaa
     8161 taaaacacag caaaagggtg ctgttaacag tgctaaagag ggtagtaacg ctggcaatgc
     8221 tgctacgaaa tcaaccaggg ctaagcatcg cggggaatat gcacagggag aggcagcggg
     8281 aaagattgcc gcaggtgata agtctgttgt gtcgaatgag ttccacgaaa cgatgattga
     8341 taaggccaac atgttgctcg atgacatcac ggagatcaac aaatgagtcc gaaagtccgt
     8401 tgcaccatag acaagcgcat gatgaataag ctgcataaag agctctcaca gatggaaggt
     8461 gtagcggttg gctgggatga cgggaaacac tatagcggcc tgaattacgc cacactggca
     8521 gctatccact ctgaggggtg gggtgatttg cctaagcgct cctttatgga atctgcccac
     8581 gcagcaatgc tctatgagag cctgagacac acaaaagccc tgattaatgc aatcgtaaag
     8641 ggtgggagct ctgaggccgt caggaagaga attggcaagc gatacgccga cattctggag
     8701 actatcctga ttagtgggca attcagcaat aacaaggttt ccccacaatg ggcaagctat
     8761 aaaggcttca atgatgccat gcgccactat gatgatttga tcggtgcaat caaatattgg
     8821 tcaaatgtgg ggagaggcta atcacggtcc ggcttaaaaa agacaatagt gttcttttga
     8881 ggccgtagtg atacaccaca aatcagcccc tttccacttc tttttctcgc gattggtccc
     8941 ttgtgagaat cctttatcag gtatgcattg tcccgtgcat gagcggtcga tagagccagc
     9001 ctctttcccg gactggttag gatttagtgc gctgagagag cgccgctatc actgttagcc
     9061 tgtttatcgt tgcctggttt gtgcatcact gcaccccctg ggcaccggac ccgcctcaaa
     9121 ggtcggatca tcacaattgg cggggcgatt ttatagtcgc tttaaggttt atagaccgga
     9181 gttgacttac cttccggcaa cctgctaaca ttagtctcgc gagaggttga tttttgttag
     9241 caggttcgcc ccgcatcaaa aggataccgg agaagagccc ctttgttaac acttaggggc
     9301 ttttttcttg cctggagttt atccgattaa gctaacttat tgattttgat cacaatatta
     9361 gaaaagctgg ttagattcct gcgtagcttt ccaatccaac tgatcgctga cttccttctg
     9421 aatcaccctg gcaattcccc cctcatcgat ggttatctcc catttgccgg aaatctcacc
     9481 ttgtggggct acatttactt ggactggttg atatccacca ttctggatag ccatgccacc
     9541 cgtcggtgtt ggtctgatgc cagtgtctac tgtcaatgtt ggccctttat taaaccagcc
     9601 tctgacctga tcgaacaaag gaccagtatc gccgccaggt gtgaacagtg cgccagcatt
     9661 gctgacaaat ctccccagct caaacaaagc accctgtttt gagttctcct tactcatttc
     9721 ctgcgtcttg ccatcaatga aacttctttc agcgttagat cctgcactga tgaccgctgc
     9781 tcctgttgcc gttccacctg aggcagaagc cagaagcttg ctcataccag ggattttacc
     9841 taacaaggct atcagctgag tgacaagtga aacaaatccg gtcagagggg aaagcagtgt
     9901 ggtaataact ttcagaccag ctaagccgac taccagtttc ccgataagct cgccaacctc
     9961 tgatccggtc atgccgaact ctttagcctt gacctcaata tatcccttag cgaagcctac
    10021 ccaatacatg atctcacgca tggcagcacc gactgcctca ccaaactgtt ttgccgcgct
    10081 cccgctgccg tccatatcag tggtgaggtc ttcgaagatg gagatcagag attcagataa
    10141 gctaataacg ccatctgcag gatctacaac acccataaac tgaatcgcca gttcgttaaa
    10201 gctgtttttc atgcgctgca ttgctacttc tggctgtttc atggattccg caaaagcttt
    10261 agtttctttt gcagcacccc agattttcac caaggaagga agaagcttat ccatggtgat
    10321 cttgccatct tcaagggcct tattaaattc ttcctctttc ttgatgattc cggcatcgat
    10381 agcggcacgg gtgaaatcta cctgagagcc tgccagggct tcagaaagtt ggcccttaag
    10441 ctcttccgca tacactcgac cgcccttgct atagatctgc cctagtgcaa cgatagccct
    10501 gttttgttgg tccgcagtag cacccactga tcggccttta atcgccatag cctcaaagag
    10561 atcctgctta tctttctctt ttatgcttgg tgtggctgca gcaaagcgca taaagccgcc
    10621 ttcagtctca cgctgatcca tcccgtagtc ataagagatt cttttgacgt aggccatctg
    10681 ctgatcagtc ttggctttat catccttgtt ctgtgccatc aaaccgaagt aggcactgtt
    10741 gcgatctgca aggctaccca tagcaccact aacagcacgc tgaacacccc agccagcggc
    10801 agtaaggcca atggctatct tgcctagtgt ggagttgagt ttgcccaggt tgtcatgcag
    10861 cttagatcct gcgatcgcag ctctgtctag tctgctctga aattttccgg cctctgctgt
    10921 tgccatcttc atttcagctc tgagccgagc cattttctga ttataggtct gcaggttaat
    10981 agccccttta gcataatcag cattaactct ctggatcctg gattggaact gagcaaccag
    11041 ggagccatca aacatgctgc tgattcgtgc actgttggcc cctacacgtg cttttctgtg
    11101 ctgaacatct ccaacactac gatctaacag tttttgttgt ctggcggcct ctctggcttg
    11161 ttgttggtag aacttgcgca tagaggagag ctccggatta ttgcccccgg tcagacggct
    11221 gacagcctga gcacgttgtt tcttctgtgc cctggcaatc tgctcttcca ccttagcgcg
    11281 tgttctttgt gttctgagca cagcctgatt agatttaaaa tcagctttag ctatatcgtc
    11341 ggtggctttc ttccaggatt ttttataatc ctcaagccgt ttaacttctt ttcgccagct
    11401 ggatttatca gaatcccagt aaataacgtt tgttagcttg gcgacttcct tcattgccat
    11461 ctcttacacc tcgccctgtc ttaaagattt tgataatgaa atagcccttt tcagggcctc
    11521 tttatcaata ttatatttct ccgcaagctc ttcgattgag aggtcaccag aggaagaagc
    11581 cccgcccttg atgacgggag cattgttatc actttttaat ttttgataaa tcttttcgta
    11641 ggtattcata tcatttgcct ttgcgtctta gtggacactt atcgccaata gtccacacaa
    11701 aaatataatg gcgcttgctt tcagctttag gatttaataa ttctttatgc aaatatccgg
    11761 ctgacagcat tttatcgacc aggtttttca ctctttgccc ttcctcaaat gtttgtgctt
    11821 tgaaatagag atttggaaag ttttggcctg tatattttcc atcctcatat aagcgggtgc
    11881 atacccagcg acataaatca tcgtcgttaa gatcactaac gtggggttta tcatcgctgc
    11941 taaataacgc ctgtttaaaa cgggagtatt taaactcccc atcttccagg aaatcaccat
    12001 catcgaattc aactatcatt tcttcaatat tcataggcat atccttttag ataaacgtga
    12061 aaagtaagat aaataaaggc acccccattt tttgagggtg cttattaatc aattattcaa
    12121 ggttaaccag cttgagaggc ttcaccgcct gcagcatggt ctgcatcatt cctacgctta
    12181 tttcaaagag atctgccatg ttctgaaggc ttgccccttc tgcctcaatc attgatttgg
    12241 ataaggcagc aatcacaaag ccatcctggg cgctgttcca catgctgata agaggatcat
    12301 caccgtgttg gcgcataagg tcctgaatca tccacagcct acatgccata accaccaaat
    12361 cgacatagaa aacatcacca ttagcggact tgcattcagc aaaagcgtgg tgtctgtgtt
    12421 ttaagaagtg ctctttgtca cgggtaaatg ccagggtgtt ggtaaacttg ccatctaaaa
    12481 gatcctggcg aacttttgaa tcttcagcgt cgaaagcttt gataacctgg gatagtaatt
    12541 tagcgatttg agacatttgg aattccttaa attagagccc cggaaggggt taaagtgagt
    12601 cgaaaataaa taaatcgaag agataaaact taggctttct gggccattgc tttccggact
    12661 ttattgctat ggcggcggta gatgaatata aattcgcgat aagcacgact accgggctta
    12721 ggcaaacgtt gattaagctc cttacgtggc aggatttcac tcaacaagcc ccagatatga
    12781 aagcaggtta cttttaattc ttttgctatc tcttcaatat tcatcccaca attccacatt
    12841 aaaagagatt tgcggcggcg atcttcgaag acatttctat ctctgtctat ttcgttgtag
    12901 acactgaggg gaactcgaca ccgtggattc caggactcat tatatagttt ttggactttc
    12961 tcaagtttaa cgcctgtata ttccgccacg aattcagggg taaagccctc gactaataag
    13021 tgtttagcct gtcgtgttgg ttgatcatca ctggggagat atttctgcag tgctaccagt
    13081 ttttcaaatt gggaatcaga aagatcagat ttgaacatga ttaattctcc gaacgtttaa
    13141 aagatcaggg caatcaatct agttctagtg cttagtttat ttatttagtt tgtttgggga
    13201 taacagggga tattaattaa taagggatgt cagaatcatt ttcttcatct gctggatctt
    13261 cagctaacca accacaatat tcttcccaat atgaaaaatg ttgtgatagt tcatcttcaa
    13321 aatatttttc cttttccagg taataatcaa aatcgccata cattttaaat ttcctttaga
    13381 atactgt
//
LOCUS       DAZFAH010000122        13372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFAH010000122 DAZFAH010000000
VERSION     DAZFAH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.122"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            501..4961
                     /gene="gltB"
                     /locus_tag="ACHJWB_003265"
     CDS             501..4961
                     /gene="gltB"
                     /locus_tag="ACHJWB_003265"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4314843.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATIKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTSGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4974..6392
                     /gene="gltD"
                     /locus_tag="ACHJWB_003266"
     CDS             4974..6392
                     /gene="gltD"
                     /locus_tag="ACHJWB_003266"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4314844.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIIEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDSKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6576..7703
                     /gene="yhcG"
                     /locus_tag="ACHJWB_003267"
     CDS             6576..7703
                     /gene="yhcG"
                     /locus_tag="ACHJWB_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO4314845.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7763..8227)
                     /gene="nanQ"
                     /locus_tag="ACHJWB_003268"
     CDS             complement(7763..8227)
                     /gene="nanQ"
                     /locus_tag="ACHJWB_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4314846.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(8224..9099)
                     /gene="nanK"
                     /locus_tag="ACHJWB_003269"
     CDS             complement(8224..9099)
                     /gene="nanK"
                     /locus_tag="ACHJWB_003269"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4314847.1"
                     /translation="MTTLAIDIGGTKLATALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(9096..9785)
                     /gene="nanE"
                     /locus_tag="ACHJWB_003270"
     CDS             complement(9096..9785)
                     /gene="nanE"
                     /locus_tag="ACHJWB_003270"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4314848.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9833..11323)
                     /gene="nanT"
                     /locus_tag="ACHJWB_003271"
     CDS             complement(9833..11323)
                     /gene="nanT"
                     /locus_tag="ACHJWB_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4314849.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQIYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11432..12325)
                     /gene="nanA"
                     /locus_tag="ACHJWB_003272"
     CDS             complement(11432..12325)
                     /gene="nanA"
                     /locus_tag="ACHJWB_003272"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4314850.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12447..13238)
                     /gene="nanR"
                     /locus_tag="ACHJWB_003273"
     CDS             complement(12447..13238)
                     /gene="nanR"
                     /locus_tag="ACHJWB_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4314851.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3205 a         3659 c         3577 g         2931 t
ORIGIN      
        1 ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag
       61 gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt
      121 atgacagtca ggaattgact gtttctctaa cgacttccct tttagcctta aagataaaat
      181 ccattttaat ttcagtaatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat
      241 aagcttgcca tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt
      301 ctggatgagc agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat
      361 taaccgatgc gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac
      421 cccgtcgcca cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga
      481 gcctggggga ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt
      541 tcggcctgat cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac
      601 acgcactggc ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg
      661 gttgcggctt gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg
      721 gctggcgttt agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac
      781 tcgccgctgc cgcacgccgc atcgttgaag aagagctgca acgcgaaacc ttgtcgattg
      841 tgggctggcg tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc
      901 tgccccgcat tgagcaaatt ttcgttaacg ctccggcagg ctggcgtccg cgcgatatgg
      961 agcgccgtct gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact
     1021 tctacgtctg tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg
     1081 atctgccgcg cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atctgcctgt
     1141 tccaccagcg cttctccact aacaccgtac cgcgctggcc gctagcgcag ccgttccgct
     1201 acctggcgca taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc
     1261 gtacctataa attccagacg ccgctgatcc ctgatctgca cgacgccgca ccgttcgtca
     1321 acgaaaccgg ttctgactcc agttcgatgg ataacatgct ggaactgctg ctggcaggcg
     1381 ggatggatat catccgcgcc atgcgtctat tagtaccacc cgcctggcag aacaacccgg
     1441 atatggaccc ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg
     1501 atggcccggc gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta
     1561 acggtctgcg tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg
     1621 aagtcggtat ctgggattat cagcctgacg aagtggtcga aaaaggccgc gtcgggccag
     1681 gcgaactgat ggtgatcgac acccgcagtg ggcgtattct gcactcggca gaaaccgatg
     1741 acgatctgaa aagccgccat ccatataaag agtggatgga gaaaaacgtc cgccgactgg
     1801 taccgtttga agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc
     1861 ttgccagcta ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg
     1921 tactgggcga aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg
     1981 tgctctccag tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga
     2041 ctaacccgcc aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg
     2101 gtcgtgaaat gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat
     2161 cgccgattct gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc
     2221 gcgcagatac gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacaatca
     2281 aagagctgtg cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgctg ctggtgctct
     2341 ccgaccggaa tatcgctaaa gatcgcctgc cggttccagc cccgatggcg gttggcgcga
     2401 tccagacccg tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg
     2461 ccagcgcccg cgatccgcac cacttcgccg tgttgctggg cttcggcgcg acggctattt
     2521 atccatacct tgcctatgaa acgctgggcc gcctggtaga cacccatgcg attgccaaag
     2581 attatcgtac cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca
     2641 tgtccaaaat gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg
     2701 tcggtctaca cgatgatgta gtgggcctgt gcttccaggg ggcggtcagc cgcattgggg
     2761 gggcgagctt tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg
     2821 cgcgcaagcc catcagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg
     2881 cctacaaccc ggacgtggtg cgcacgctgc aacaggcggt acagagcggc gagtatagcg
     2941 actatcagga atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc
     3001 tggcaattac gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac
     3061 tgtttaaacg cttcgatacc gctgcgatgt ctatcggcgc gttaagccca gaggcccacg
     3121 aggcgctggc ggaagcgatg aacagcatcg gcggtaattc gaactccggt gaaggcggcg
     3181 aagacccggc gcgctacggc accaacaaag tgtcgcgcat caagcaggtg gcttccggtc
     3241 gctttggggt tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc
     3301 agggcgcgaa gccaggcgaa ggcggtcagt tgccgggtga taaagtcact ccttacatcg
     3361 ccaaactgcg ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata
     3421 tctactctat cgaggactta gcgcagctca ttttcgacct caagcaggtt aacccgaaag
     3481 cgatgatctc cgtgaagctg gtttccgaac cgggagtagg caccatcgcg actggcgtgg
     3541 caaaagctta tgcggacttg atcaccatcg caggctatga cggcggcacc ggcgcaagtc
     3601 cgctttcatc ggtgaaatac gcaggctgtc cgtgggagct ggggcttgtt gaaacccagc
     3661 aggcgctggt tgctaacggc ttgcgtcaca agatccgttt gcaggtcgat ggcggcctga
     3721 aaacgggtgt cgatatcatc aaggcggcga ttctcggcgc agaaagcttc ggcttcggca
     3781 ctggcccgat ggtggcgctc ggctgtaaat atctacgtat ttgccatctg aacaactgcg
     3841 caacgggtgt agcaactcag gatgacaaac tgcgtaagaa ccactatcac ggcctgccgt
     3901 tcaaggttac caattacttt gaatttattg cccgtgaaac ccgcgagctg atggcacagc
     3961 tgggcgtaac gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gaactggacg
     4021 gtttcaccgc caaacagcaa aaactggcgc tgtcgaagct gctggagacc gccgaaccgc
     4081 atccaggtaa ggcactctac tgcaccgaaa acaacccgcc gtttgataac ggcctgctga
     4141 acgcgcagtt gctgcaacag gcgaaaccgt ttgtcgatga gcgccagagc aaaaccttct
     4201 ggttcgatat tcgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc
     4261 agacgcacgg cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg
     4321 caggccagag cttcggcgtg tggaacgcgg gcggcgtgga actgtacctg accggtgatg
     4381 ccaacgacta tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg
     4441 gttccgcctt ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga
     4501 ccggtggtcg tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg
     4561 gtgctatcac cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgagcggta
     4621 tagtctgtat tctgggtaaa accggcgtga acttcggcgc gggcatgact ggcggcttcg
     4681 cttacgttct cgatgaaagc ggcgatttcc gcaaacgcgt taacccggaa ctggtcgagg
     4741 tcttaagcgt tgacgctctg gcgatccatg aagagcatct gcgtggtctt atcaccgagc
     4801 atgtgcagca taccggctct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg
     4861 ccactaaatt tgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc
     4921 gtagtcgtag cgcagctgag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc
     4981 agaatgttta tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga
     5041 tccgcaaaat tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc
     5101 aggctgaccg ctgcctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca
     5161 actacatccc gaactggctg aagctcgcca acgaggggcg tattattgaa gcggcggaac
     5221 tgtcgcacca gaccaacacc ctgccggaag tttgcggacg agtctgcccg caagaccgtc
     5281 tgtgcgaagg ttcctgcact ctgaacgatg agtttggcgc ggtgaccatc ggcaacattg
     5341 agcgttatat caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga
     5401 agcaaaccgg taaaaaagtg gcgattatcg gcgcaggccc ggcaggtctg gcgtgtgcgg
     5461 atgtcctgac gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg
     5521 ggctgctgac cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgccgcc
     5581 gtgaaatctt caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg
     5641 tgcagctgga cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc
     5701 agtcaatgcg cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt
     5761 tccttatcgc caacaccaaa cagttaatgg gctttggcga aactcgtgaa gaaccgttcg
     5821 tcagcatgga aggcaaacgc gtggtagtac ttggcggtgg cgataccgcg atggactgcg
     5881 tgcgcacgtc tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag
     5941 agaacatgcc gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggt gttgagttca
     6001 aattcaacgt ccaaccgctg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa
     6061 aaatggtgcg taccgaaatg ggcgaaccag actctaaagg ccgtcgccgc gcggagatcg
     6121 tggcagggtc agaacatatt gttccagcag atgcagtgat catggcgttt ggttttcgcc
     6181 cacacagcat ggaatggctg gcaaaacaca gcgtcgagct ggattcgcaa ggccgcatta
     6241 tcgctccgga aggcagcgac aatgctttcc agaccagcaa cccgaaaatc tttgccggcg
     6301 gcgatatcgt ccgcggttcc gatctggtgg tgaccgctat tgccgaaggc cgtaaggcag
     6361 cagacggtat tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa
     6421 aagagtcggc gctgaaattc tcctttcttt ctggaaagcg cctcgcataa tctgaagaaa
     6481 accaatgtaa tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc
     6541 attattcaac gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct
     6601 caacaggcta ccagcaaatc cacgacggta ttattcatct ggtcgatagc gcccggacgg
     6661 aaacggtacg tagcgttaac gcgctgatga ccgcgacata ctgggaaatt ggccgacgaa
     6721 ttgtcgaatt tgaacaaggt ggcgaggcca gggctgcgta tggtgcgcag ctaatcaagc
     6781 gactatcaaa ggatttaagt ctaaggtata agcgtgggtt ctctgcaaaa aacttacgcc
     6841 aaatgaggct tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg
     6901 aattaacacc attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt
     6961 ggcagacgct gtctgccaaa tctttccccc tgccctggtc cacctacgtc cgtttacttt
     7021 ccgttaaaaa cgctgacgcc cgcagctttt atgaaaaaga gacgctccgc tgtggctggt
     7081 ctgttcgcca gctagaacgg caaattgcga cccaatttta tgagcgaaca ctactgtcac
     7141 atgacaaatc agccatgctg caacaacacg ctcctaccga gacgcatatt cttccgcagc
     7201 aggcgatacg cgatcccttt gtgctcgaat ttctggaatt gaaagatgaa tactcagaat
     7261 ccgattttga ggaggcgctg atcaaccacc tgatggattt catgctggaa cttggggatg
     7321 attttgcctt tgttggtcga cagcgaaggt tacgcattga tgacaactgg tttcgtgtcg
     7381 atctgctgtt tttccaccgc cgtttacgct gcctactaat cgtcgatcta aaagtgggca
     7441 aattcagcta tagcgatgcc ggacagatga atatgtatct caactacgcc aaagagcact
     7501 ggacgctacc ggatgaaaat ccgcccatcg gtctggttct ctgtgcagag aaaggagccg
     7561 gagaagcaca ttatgctctg gcaggtttgc ctaacaccgt tctggcaagc gaatataaga
     7621 tgcaactacc tgatgagaaa cgactcgcgg atgaactcgt tcgaacacag gcggtgttag
     7681 aggaaggcta tagacgccgt taatttcagc ggtttcgaat tctgcagacg gtgtctgcag
     7741 aatctttaaa acgaactaag ttttaagcca ttaaatcagc cttaaccttc accaccacct
     7801 ttttaatctc accaggttcg ccaacaacgc atcctggttt atgcggttca cctggcataa
     7861 acacggcgaa cattcccggt tttaagatga tggcttgctc gttatcaatg gtgctgcaaa
     7921 gctggtaatc atcttcatgg tggaactctt cacactgacg cgcagtgcct gccatgccaa
     7981 acagaatccg ttcctcaccg tttaataaca gctggatatc aatgtattgc tcgtgcaatt
     8041 ccgctttttt ctcgacgggc gattgagtgt taaacgtcat gacattcata aagatattgt
     8101 cgccctgtaa ttcgtaacga cccggcgctt tttcttgcgg tctggcagct aatgccagcg
     8161 ttaacgcgtc ctgtaacgca ggatgtaacc cagcagacgg taatgactgt acttcaccca
     8221 tcatcataat ttttctccct gggccaacag cgcagcccca agtaaacctg catcatggcg
     8281 gtaatgcgcc gccagtaaat caacatgaaa tgccgctggc tcctgcgcca gatacgtttc
     8341 caccaacgcc agataccctt ctgccagacc aacgctgcca ccgaccacca cacactggca
     8401 atcagttgtg gctttaatat cagcgatcag ccttgccagc acatgtgcgg agcggtgaat
     8461 cagttgctgc gcctgctcgt cgccctgccc ggcgcgtgtg aaaatagttc tcgcatccgc
     8521 gcccatgagt tcaccttgcg ctgccgccgc aatgccgcga ccagaagcaa tcgcttccac
     8581 gcaacctgtg cgtccacagc cgcagactgg accatgcgga tcggcaagcg tatgcccgat
     8641 atgccccgcc agaccgccag ggccggtaag cagtttaccg ccgctcacta caccgccgcc
     8701 gacgccagtg gaaacggtga taaacaccat ctcggttaca tcgccttcca gcgcctgata
     8761 ctctgcccat gctgcggcct gcgcgtcgtt aatggcaatg gtcggcaaat tggtaagttg
     8821 ttccagcgtt ttgactaacg gaaagtgtag caatccaccg agattatgcg gattcagcgc
     8881 cagcaaactg ccgtcgcgaa taattccggt tgaagcgatg gcaacccgct gcgcatgagc
     8941 ttgcaacgga gagactaatg cggataaggc atcacgcaag gcttctggtg tctggctggc
     9001 tggcgtagga agttcacgac gatcgcggat ctgcccgtca gcgccaatca gcgcggtggc
     9061 aagtttagta ccgccgatat caatcgccag tgtggtcata gcaccgcctt tttcatcgct
     9121 gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg cagaaccgac cgtcaccgcc
     9181 cacgcgccgt ggcgcatcgc atctgccgcc tgagcaggcg tgttgtaacg cccttcggca
     9241 atcacccgac atccggcgtc gctcaacgtt ttcaccagcg ccagatccgg ctcttctggc
     9301 gtttcaggcg tggtatagcc agaaagcgta gtgccaataa tttcggctcc cagcttttgg
     9361 catgccaggc cgtcttccgg cgttgagcag tcggtcatcg ccagtaaacc gtgatggtga
     9421 atacgtgcca gcagcgtttc aacaggcacc ggacgcgggc ggtcggtgcc gtcaatggcg
     9481 ataatgtccg cgcccgcctg cgccagcgca tcgacatctt caatataggc cgtgatgcgt
     9541 accggagaat cctccagatc gcgtttcaca attccaataa tcggcacgct caccaccgca
     9601 cgcgtggctt gcagatttgc cacaccttca atacgaatgg cgaccgcgcc cgcctgctct
     9661 gccgctaatg ccatggctgc gacgatttcg ggtttatcga gcgggctgtc aggaaccggc
     9721 tggcaggaga caatcaggcc accgttagca gcgatttttt gatccagttg tgcaagtaac
     9781 gacatacatc ttcccttagc gaaaggcccg gtacagagac cgggcaacag gattaacttt
     9841 tggttttgac taaatcgttt ttggcgctgc caaacggcac ggcaccgctg aatggtttac
     9901 cgtcgatagc gtcatgagta cgcaacgctt ccgggcgcaa ccaacgctga acgcgagaag
     9961 gcatatccag cccaatcagc aggatcacca cgaacgtcag actgaacgag agcgatgcca
    10021 gcgcagtacc cagatccaga cgttgagcga tcaacgcgcc gatgattggg gccagtgcac
    10081 cgcccaatgc gccaacgttg taggtaaagc ccaggcccgc tgcacgctgg tcggtatcga
    10141 aataaccgcc aatcagtttt ggtaagatcc cggcgatccc ttgtccaagc atttgctgga
    10201 agaacagtaa cagaccgagc acccagacgt ttgcgccgcc aatcgcaaat accggaataa
    10261 tcagcagctg cgaggccagc aggctacaaa cgtacgcttt gcgggttccc agccagtcac
    10321 cgaggaagcc acctacgcag catcccaccg ccgcgccaaa gccactaaag aacagcacat
    10381 tggctacagt atgcgggtta taagccagat cggttttcag atacgttggc agcagcgcct
    10441 gaatcggcca tgagtagagg aaagcaaaca acacgaccac catcagcatt acgcccgttg
    10501 gccagcgttt gcctgtactc tgcaccataa agctgataaa gattgcggcg cataacagcc
    10561 caagaacagc gacgatcgcg gcattttgca ggttaccggc gaagcagaac cacagcgcag
    10621 tagccgccgc cagtgtcatt acgatattgg caatgcgatg ttcaccacgg tagagaatat
    10681 ccaccattgt gcgtactggt gctttacctg cgtgtttctc tttccagtct tccgcttccg
    10741 ggatgttttt acgcagccag agagcaaaga tgattggcaa aatgccgata aagaacagcg
    10801 cacgccagcc ccagaccgga accaccaggc tatagatctg agcggcaacg acggccccca
    10861 cagagaagcc tgaaatcaga aaaccactgg ctttgttacg cagatgtttt ggccagcttt
    10921 caatgacata ggtggcgctg gaaccgtatt cacccgccat ccccatgccg atgaccagac
    10981 gcgcgataaa catggtgatg tagcctggcg caaagccgca ggccagcgtc ccggccgaga
    11041 agagaacgat gctggtgacc attgccagac gacgcccgta gcggtcaccc atagcgccga
    11101 gcatcaggcc gccgaaccag cgagagataa aggctgcgga gatcagactt gccgcctgca
    11161 ccgtcgtcag cccgaattca ccctgtactt cggtgagtac cagggcgatt aaaacgaaat
    11221 caaaaccgtc aagcagatat cccaaccagg cagcggaaaa tgcgcgccat tgtgcacggt
    11281 tgagatggcg ataccacggg atattctggg ttgtagtact cattgtgagt ctcccgcggt
    11341 gggcgatgcc cacacgcttt ggtataaaaa ttgtagggta cagatgcgtt tatttcccct
    11401 cacccggtag gggcgagcga ggggaaacaa ctcacccgcg ctcttgcatc aactgctggg
    11461 ccagcgcctt cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca
    11521 gcggcacaga aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg
    11581 ttttgatcag taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga
    11641 tatcgccttc tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg
    11701 tactaccgat accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac
    11761 cgttatagag cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc
    11821 cagaggtctg tttcagcgca cctacgccag gcaatgtaac aagtgtgttg atctgatcca
    11881 gggtcagttt taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg
    11941 ccgaatcaat aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga
    12001 acggcgtgac ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt
    12061 ggctttcggc ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct
    12121 cttcggcgac gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc
    12181 cggtcgaacc acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc
    12241 gacgcagact cgctttatcc agtgcttgtt gttggtcaaa aggagtcagg agtgcagcca
    12301 ttacgccacg taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga
    12361 tataccttca tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata
    12421 atgcgatctg cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg
    12481 tgccaggtag cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga
    12541 tcatgacggc ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt
    12601 tcgtgcagtg cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca
    12661 acgtggatcg ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca
    12721 acgtctgaac gaatgaatgc cgcgttgtta tccagcgact gactgttgat ttccagtgct
    12781 tttgccagca aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccagactg
    12841 gattcaaaga acagacgtaa ttgttcgaaa tgggcaatcc caccgggatg agaaaggaaa
    12901 tctttcgcca tgccggaaag ctcaccgatg atagtgtccg cagaaggacg cgagacgcga
    12961 gcgcgttcgc cgttgtttat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttca
    13021 cgcaccgaag gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt
    13081 tcaccttcgc caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg
    13141 gagagttttt tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa
    13201 tcttcggttt gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa
    13261 catcatcagt gacatcctat cacaggattg aaagtagggg aaaatggcag ggttttctct
    13321 ttgtgcctca tcattaccat aattaacgga ataattaact attgcgaaaa at
//
LOCUS       DAZFAH010000123        13271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFAH010000123 DAZFAH010000000
VERSION     DAZFAH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.123"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..759
                     /gene="ydiV"
                     /locus_tag="ACHJWB_003274"
     CDS             46..759
                     /gene="ydiV"
                     /locus_tag="ACHJWB_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4314852.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            822..2258
                     /gene="selO"
                     /locus_tag="ACHJWB_003275"
     CDS             822..2258
                     /gene="selO"
                     /locus_tag="ACHJWB_003275"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4314853.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2262..2453)
                     /gene="hemP"
                     /locus_tag="ACHJWB_003276"
     CDS             complement(2262..2453)
                     /gene="hemP"
                     /locus_tag="ACHJWB_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4314854.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2585..3631)
                     /gene="aroH"
                     /locus_tag="ACHJWB_003277"
     CDS             complement(2585..3631)
                     /gene="aroH"
                     /locus_tag="ACHJWB_003277"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4314855.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3788..4621)
                     /gene="ppsR"
                     /locus_tag="ACHJWB_003278"
     CDS             complement(3788..4621)
                     /gene="ppsR"
                     /locus_tag="ACHJWB_003278"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4314856.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4954..7332
                     /gene="ppsA"
                     /locus_tag="ACHJWB_003279"
     CDS             4954..7332
                     /gene="ppsA"
                     /locus_tag="ACHJWB_003279"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4314857.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYISDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7389..9051)
                     /gene="fadK"
                     /locus_tag="ACHJWB_003280"
                     /pseudo
     CDS             complement(7389..9051)
                     /gene="fadK"
                     /locus_tag="ACHJWB_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(9108..9401)
                     /locus_tag="ACHJWB_003281"
     CDS             complement(9108..9401)
                     /locus_tag="ACHJWB_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4314858.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDTGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9398..10687)
                     /locus_tag="ACHJWB_003282"
     CDS             complement(9398..10687)
                     /locus_tag="ACHJWB_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4314859.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKREQEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10743..11681)
                     /locus_tag="ACHJWB_003283"
     CDS             complement(10743..11681)
                     /locus_tag="ACHJWB_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4314860.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIVEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11701..12465)
                     /locus_tag="ACHJWB_003284"
     CDS             complement(11701..12465)
                     /locus_tag="ACHJWB_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4314861.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            12781..>13271
                     /locus_tag="ACHJWB_003285"
     CDS             12781..>13271
                     /locus_tag="ACHJWB_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO4314862.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYE"
BASE COUNT         3210 a         3312 c         3427 g         3322 t
ORIGIN      
        1 ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa gatttttttg
       61 gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca gcaactcctt
      121 gtcggcgttg agttaattac ccatttctcc agtgaagatg gcacggtgcg tatcccgacg
      181 agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc agagcagttg
      241 gaattactaa aatcatgcca acattttttt attcaacata aactctttgc ctggcttaat
      301 ttaacgccac aagtggctac attattatta gagagggata attacgcagg cgaactatta
      361 aaatatccgt ttattgaact gttgattaat gagaattacc cgcatctcaa tgaaggaaaa
      421 gacaatcgcg gtcttttatc cttatcgcaa gtgtatcccc ttgtgttagg taatttggga
      481 gcaggcaata gcacaatgaa agcggttttt gatggtcttt tcacccgcgt catgctggat
      541 aaaagtttta ttcagcaaca aatcacgcac cgctccttcg aaccgtttat tcgtgcgatc
      601 caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg gaggcattga tacggcagaa
      661 atattggcac agattacccc cttcgatttc catgctctcc agggatgcct gtggcctgcc
      721 gttcctataa atcagataac gacgttggtt cagcgataac cctcctgttt gctggtgttt
      781 aagacgagag taaccgtcta cactatcaaa caggaggatc tatgaccctg tcttttatta
      841 cccgctggcg cgatgaattg ccagaaacct atacagcact ttcccctacg cctttaaata
      901 atgcccggct gatttggcat aataccgaac tcgctaacac gctgagtatt ccatcgtcgc
      961 tgtttaaaaa tgctgcaggt gtctggggcg gcgaaaccct actgcctggt atgtcaccac
     1021 tggcgcaggt ttacagtggt catcagttcg gcgtctgggc gggccaactg ggtgatgggc
     1081 gcggcatttt acttggcgaa caactgcttg ctgatggcac tacaatggac tggcatctga
     1141 aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg acgggcggtt ttacgttcga
     1201 cgatacgaga aagtctcgcc agtgaggcga tgcattatct gggcattccg acgacccgcg
     1261 cgttaagtat cgtcaccagc gattcgccag tgtatcggga aacggtagag ccaggtgcga
     1321 tgctgatgcg tgtggcacca agtcatctgc gctttggtca tttcgaacat ttttactatc
     1381 gccgcgagcc ggaaaaggtt cgtcagttag ctgactttgc cattcgtcat tactggtcac
     1441 atcttgaaga tgatgaggac aaataccgtc tctggtttaa cgatgttgtc gcacgtaccg
     1501 catcgttaat tgcccaatgg cagacggtcg gctttgctca tggggtgatg aataccgaca
     1561 acatgtcgct gctggggctg acgcttgatt acgggccgtt tggttttctt gatgattacg
     1621 aacccggttt tatttgtaat cactcggatc atcaagggcg ttacagcttt gataatcaac
     1681 ctgccgtcgc gttgtggaat ttacagcgtc tggcgcagac attgtcacca tttgttgccg
     1741 tagatgccct gaatgaggcc ctggacagct atcagcaggt tttgttgacg cattatggac
     1801 aacggatgcg gcagaaactg ggcttcatga cggagcaaaa agaggataac gcgctactga
     1861 atgaattatt cagtctgatg gcgcgagagc gcagcgatta tacccgcaca ttccgcatgc
     1921 tgagtctgac cgagcagcac agcgcggcgt caccgctacg tgatgagttt attgatcgtg
     1981 cggcatttga tgactggttt gcccgttatc ggaggcgttt gcaacaagac gaggttagcg
     2041 atattgagcg tcagcaactg atgcaaagcg ttaacccagc tctggtgttg cgcaactggt
     2101 tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat gacggaattg caccgcctgc
     2161 atgaggcgtt gcgaaatcct ttcagcgaca gagctgatga ctatgtcagt cgtccacctg
     2221 actggggtaa acggctggaa gtcagctgct cgagttaaag gctacttggt caacagcagc
     2281 ttgccagcct gagttttacg gagcagatat tcttgcccgt catgatcgat aatcagtttg
     2341 ccatccggac ctaacagtgt ctggctggta attcgccgga tgggctgcgg ggaagcggtc
     2401 ttgtgattgg catccgtttc aggcgtgagt tttctgctat ccgtataacg cataaaaatt
     2461 aataataatt gatataacaa tgattcttat tatcaatatt atggcaggcc agagcaagca
     2521 gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc atcccatcag gaatgggcac
     2581 gcattcagaa gcgggtatct accgcagagg cgagtttttc gaccaggcgt tcggtatctt
     2641 cccagcccag acacgggtcg gtaatggatt gaccgtaagt gagcggctga ccgccgacga
     2701 ttttttgcgt tccttcgcgc aggaaacttt ccgccataat tccagcaatc gccgtagagc
     2761 cattgcggat ttgctgacaa atatcctcac aaacttctaa ctggcgacgg tgctgcttct
     2821 ggcagttacc gtggctgaaa tccaccacca gatgttcagg taaatcaaac tcgtgcagcg
     2881 tatcgcaggc tgcggcgata tcatcggcat gataattcgg ttttttgccg ccacgcataa
     2941 taatgtggcc atacgggttg ccgctggtct gatagatggt catctgacca tttttgtctg
     3001 gcgagaggaa catatggctg gcgcgggctg cgcggatagc atccacggca atccgcgtgt
     3061 tgccatcggt accattttta aaacctaccg gacaggagag tgctgaagcc atttcgcggt
     3121 ggatctgact ttcggtagta cgagcgccaa tcgcgcccca actgattaaa tcagcaataa
     3181 actgaccggt caccatatcg aggaactcag tcgcggtagg gacacccagc tcatttacct
     3241 gtaaaagtaa tttgcgcgcc agctccagac cgtgattaac ccgatagctg ccgtttaaat
     3301 ccggatcgga gattagccct ttccagccga caacagttcg tggtttttca aaataggtgc
     3361 gcattacgat ttccagccgt gactggtact ggttgcgcag cgactgcaga cgggtggcgt
     3421 actccattgc agcggtgaga tcgtggatcg agcaggggcc aataatgacc aacagtcgct
     3481 tatcttcacc attcagtatt ttttcaattc tgcggcggga gtcggtgaca tgggtggcga
     3541 cgccaggcgt tacgggatac cgtagcgcga gttcggcggg cgttaccagg ctctcaatac
     3601 gcgcagtacg gagttcgtca gttctgttca ttacaagtct cagaaatttg ttgcttctac
     3661 ggcaggacta ccgggaagtg atgcgcatca cgataaacca atcgcagctg atttcaagtg
     3721 cgaggtgtgt aacacctcgc cgggtggtta gcatgataac aaaaaaataa gctaatgcac
     3781 tagttctcta gtacattcgg cggctaagcc ccatgatatc gaggatcttg gtggcaatct
     3841 cttctaccga ataattggta ctgttaatcc acgggatctg atttttacgg tacaacgctt
     3901 ctacttccgc gacttccatc ctgcactgac gaagcgaggc atagcggctg ttctcccgac
     3961 gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt caggccgaac aatttatgct
     4021 gaagcggttt gagcgacgcg ggtagcacca gattgtccat atcgtcggca ataaaggggt
     4081 agtttgccgc gcggatacca aactgcattg ccagatacag actagtgggg gttttaccac
     4141 agcgagaaac accgagcagg atcacctgag cctggtccag attgcgcaac gaaatgccgt
     4201 catcgtgggc gagggtgtaa tcaatcgccg caatgcgcgc atcatattta ttgagattat
     4261 tagggttaag gccatgggta cgatgagcaa tcggcgttgg atccagtttc atctcttgtt
     4321 gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa gccttcactt tgcaagatga
     4381 tggcgcgaat ctccggcaac acgatggagt agaagaccag cgggcgcaca cctgtctggt
     4441 gataaattgc gtcaatctga tccttcactg cccgtgcacg gctctcattt tcgacaaacg
     4501 gcagcgtaat gctgctgata gtgacgggaa attgtgacat tactgcgtgt cctaatacct
     4561 ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg gcgatcaaca gcattatcca
     4621 ttttgttctt cccgtgatgc agacataatt ctatgaaagc ataaattaaa aacgcacaga
     4681 agcgtagaac gttatgtctg gtttataaaa tgaaccttca attttatttt ttatgaaaac
     4741 agcatttcaa ttttatggtt tcgtttatac cgatggttta tgtggaaatt gtcgaagaga
     4801 gcagatttgc gcaacgctgg gatcagtctt aaaaagtaaa aaaatatatt tgcttgaacg
     4861 attcaccgtt tttttcatcc ggttaaatat gcaaagataa atgcgcagaa atgtgtttct
     4921 caaaccgttc atttatcaca aaaggattgt tcgatgtcca acaatggctc gtcaccgctg
     4981 gtgctttggt ataaccaact cggcatgaat gatgtagaca gggttggggg caaaaatgcc
     5041 tccctgggtg aaatgattac taacctttcc ggaatgggtg tttccgttcc gaatggtttc
     5101 gccacaaccg ccgacgcgtt taaccagttt ctggaccaaa gcggcgtaaa ccagcgcatt
     5161 tatgaactgc tggataaaac ggatattgac gatgttaccc agcttgcgaa agcgggcgcg
     5221 caaatccgcc agtggattat cgacactccc ttccagcctg agctggaaaa cgccatccgc
     5281 gaagcctatg cacagctttc cgccgatgac gaaaacgcct cttttgcggt gcgctcctcc
     5341 gccaccgcag aagatatgcc ggacgcttct tttgccggtc agcaggaaac cttcctcaac
     5401 gttcagggtt ttgacgccgt tctcgtggca gtgaagcatg tatttgcttc tctgtttaac
     5461 gatcgcgcca tctcttatcg tgtgcaccag ggttacgatc accgtggtgt ggcgctctcc
     5521 gccggtgttc aacggatggt gcgctctgac ctcgcatcat ctggcgttat gttctccatt
     5581 gataccgaat ccggctttga ccaggtggtg tttatcactt ccgcatgggg ccttggggaa
     5641 atggtcgtgc agggtgcggt taacccggat gagttttacg tccataaacc gacactggcg
     5701 gcgaatcgcc cggctatcgt gcgccgcacc atggggtcga aaaaaatccg catggtttac
     5761 gcgccgaccc aggagcacgg caagcaggtt aaaatcgaag acgtaccgca ggaacagcgt
     5821 gacatcttct cgctgaccaa cgaagaagtg caggaactgg caaaacaggc cgtacaaatt
     5881 gagaaacact acggtcgccc gatggatatt gagtgggcga aagatggcca caccggcaaa
     5941 ctgttcattg tgcaggcgcg tccggaaacc gtgcgctcac gcggtcaggt catggagcgt
     6001 tatacgctgc attcacaggg taagattatc gccgaaggcc gtgctatcgg tcatcgcatc
     6061 ggtgcgggtc cggtgaaagt catccatgac atcagcgaaa tgaaccgcat cgaacctggt
     6121 gacgtgctgg tcactgacat gaccgacccg gactgggaac cgatcatgaa gaaagcatct
     6181 gccatcgtca ccaaccgtgg cggtcgtacc tgtcacgcgg cgatcatcgc tcgtgaactg
     6241 ggcattccgg cggtagtggg ctgtggtgat gcaacagaac gaatgaaaga cggtgagaac
     6301 gtcactgttt cttgtgccga aggtgatacc ggttacgtct atgcggagtt gctggaattt
     6361 agcgtgaaaa gctccagcgt agaaacgatg ccggatctgc cgttgaaagt gatgatgaac
     6421 gtcggtaacc cggaccgtgc tttcgacttc gcctgcctgc cgaacgaagg cgtgggcctt
     6481 gcgcgtctgg aatttatcat caaccgtatg attggcgtcc acccacgcgc actgcttgag
     6541 tttgacgatc aggaaccgca gttgcaaaac gaaatccgcg agatgatgaa aggttttgat
     6601 tctccgcgtg aattttacgt tggtcgtctg actgaaggga tcgcgacgct gggtgccgcg
     6661 ttttatccga agcgcgtcat tgtccgtctc tctgatttta aatcgaacga atatgccaac
     6721 ctggtcggtg gtgagcgtta cgagccagat gaagagaacc cgatgctcgg cttccgtggc
     6781 gcgggacgct atatttccga cagcttccgc gactgtttcg cgctggagtg cgaagcagtg
     6841 aaacgtgtgc gcaacgacat ggggctgacc aacgttgaga tcatgatccc gttcgtgcgt
     6901 accgtagatc aggcgaaagc ggtggttgag gaactggcgc gtcaggggct gaaacgtggt
     6961 gagaacgggc tgaaaatcat catgatgtgt gaaatcccgt ccaacgcctt gctggccgag
     7021 cagttcctcg aatatttcga cggcttctca attggctcaa acgacatgac gcagctagcg
     7081 ctcggtctgg atcgtgactc cggcgtggtg tctgaattgt tcgatgagcg caacgatgcg
     7141 gtgaaagcat tgctgtcgat ggctatccgt gccgcgaaga aacagggcaa atatgtcggg
     7201 atttgcggtc agggtccgtc cgaccacgaa gactttgccg catggttgat ggaagagggg
     7261 atcgatagcc tgtctctgaa tccggacacc gtggtgcaaa cctggttaag cctggctgaa
     7321 ctgaagaaat aaaataaata cccggcggcg ttttgtcgcc gggtttatgt gatccccgaa
     7381 gatgaaactt attcaatctc ttcacagaca tcctgagtta atcgccgcat aatatctttc
     7441 cttaacaaaa acttttgtat tttacctgag gcagtgcgcg gtagtttttc gattaccacg
     7501 atatgttcag gatatttata ttttgcgacc cgtttacggc taaaaaaagc caccacctct
     7561 tccagcgata atgaatgatg cggcgctttc agcacgacat aagcgcatga tcgttcacct
     7621 aaacgttcat cgggcattgc aaccacacag gcatcgtgaa ttttaggatg ctgcaataaa
     7681 atatcttcca cttcaaggct gctaatattt tcgccgccgc ggacaataat atcttttttg
     7741 cgtccggtaa tttttatata gccagcctca tccatacggc agagatcgcc gctgtaatac
     7801 cagccttctt catccagcgc acggacggtt aattcaggtt catcaaaata tcccataaac
     7861 acattggggc cacgcgaggc ttcttgacct tcgcaacctg gcggtaaggt cttgcgtgcg
     7921 tcatcgacca ctttaatctc tacacctgcg gcagcgtaac catcggtgtg cataaagcgc
     7981 gacaaaggat catcgagatt caccaccgca tgcggcgaac tttctgtgga accataaaca
     8041 cttaataatt taatgctgcg ctgctggcat tcacgcgcca cttttttggg gattgtggtg
     8101 ccaccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gctgtttctc tagcaaattc
     8161 aaaagatcat agacaaacgg cgttgcgccg agcatacagg tgcaacgctg ctgctcaagc
     8221 agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc accaatcaaa
     8281 aatggtgctg ttacgccatg cagaaagccc gttgcgtggc cgagtggcgc aggcatcata
     8341 aagacatcct gccaggtcag attcagccgc gcgcaataag cccgctcact ggcgagaata
     8401 ttgttatgcg ttagcatcac gccctttggc agaccctcgg ttccggaggt aaacagcacc
     8461 gcagctaatt catcgccgtg ggtcgttatc gccgtgggtc gttatcgccg tggtcagtgg
     8521 ggtattgtcg gcgataatct gacttaatta gagggaagag gtggcgggag ccagtttgtc
     8581 cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac
     8641 cgggctcgtt tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag
     8701 cacccacacc agttctgctt cccgccagga aggcaacagc ggcacggaaa ccgcaccgat
     8761 tttcaggcag gcaagataga taacggtaaa ttcacaccag ccaggcagtt gaaatgcgat
     8821 gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg
     8881 atcgagcgcg ctataggtgt acgatgcacc atgattatcg accacggcaa ttttgtctgg
     8941 catcgcacga gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc atagcccttg
     9001 ctgacgatac gccgcacgac gttgttcgtt aaacgttaat gtcactttca ttttggactc
     9061 tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga
     9121 aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttaccgcac
     9181 agcacccgac aggtgccgca ctccagacaa ccggcggaat caaaatgaat gtttcctgtg
     9241 tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata
     9301 tcgggatttg ccgccagaat gatatgcggg tggccctcat caacatggaa tttattgacg
     9361 cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa
     9421 tgccatcttt cagcaagttg atcagcccaa ttttcttcgc gtgtcccatg atcattttgc
     9481 gtaccggctg gtttggtttg ccgtcaatgg tgaacatctc gttcatgatg tcggcgacca
     9541 ttcgtgggta ttggctaaac aggcgcgggt tttccatcag cgccgggatc ttgcggaaat
     9601 gctgcatatc gcgcatgacg cagctttgtt cctgctcacg tttgtattgc gccagactgc
     9661 tggcggagaa atcctcgcgt tctttggcgg cgatcaccgt tgtggcggca gcctgagccg
     9721 atgcaatggc taaatccatg ccacgaactg taaaacccaa attcaggcag aagcctgcgg
     9781 cgtcaccaac gatcatcacg ccatcgttaa ccagctgcgg caccattgcc agaccgcctt
     9841 ctggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaattagt gggcgaatgg
     9901 cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca tgggcgatat
     9961 cacccaatcc acaaaccagc cccaacgata cggaatcgtt gttggtatag aggaatccgc
    10021 cacccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg
    10081 taatattaaa gcgatcgttg atctgttctg gcgtgaggcc aataacctct ttaacaccaa
    10141 cagcgtaatg atgcggatcg gaagcgggaa ccattcccag cgagcggcca agcatcgagt
    10201 taacgccatc agccagaatc accacattcg cttcgagaat atcatcccca gcctgcacgc
    10261 cagttacctt gtttccttca cgaaccagcg catcgacgcg aacgcctggg ataaactgcg
    10321 cgcccgcctg ctcagcttgt tccatcaacc acgggtccag acgattacgc aatacggtat
    10381 aagatgcgtg ttgcggaaca tctggctgct cgcggtgaaa atcgagggta acggcgcttt
    10441 cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatgctg
    10501 caaagcctgg aatgattgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt
    10561 tacatccggc actgtcgccg cgttctatca ccaggacatc cagtccggct cgtgccatga
    10621 ccagtgcggc aacgctacca gcaacgcccg caccgaccac aatggcatca aatttgtcat
    10681 ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg
    10741 aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc gcccaccaga
    10801 ccgtagtcgg catagttgaa gattggcgca tttttatctt tattgatggc gacaatcact
    10861 tttgcgccgt tgccgccaac catatgttgg atctgcccgg agatccccag cgtcaggtag
    10921 agatcagatt tcagcaacac gccggagaca ccgatataac gttcacgctc catccagttc
    10981 tcgccttcga caattggacg tgaacagccg acttcagcat tcagcaccgc cgccagttcg
    11041 tggaccattt tcaggtcatc ctgcgccgca agaccacgac cgacgccaac cacacgtttt
    11101 gctttgctca ggtccacgct gcttgcggct ttcgcacggc gttcgcgaca gagaatttca
    11161 tgacgcggag caacataagg taccgtttct gtcgggcact gatgagaggt atcactggtg
    11221 cacggttcct gaacaccggg tgcaagggta atgatcgcca gcgggctgtt gatcttttcc
    11281 tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc atcgacaata
    11341 tccaccgccg tggcatcgtt caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc
    11401 agagctttac cacgtttcgt cgcggccaac agcaacatag cggggtgttt atctttcagc
    11461 agggcagcaa tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga
    11521 acataaatac attttggacc ataaggcata actgcctgcg cctggtcggt attttgtaca
    11581 atggcataca cctgttggcc ccattgctga gcgccgccaa acagttcagc ataacgttca
    11641 ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc tccgtaggct
    11701 tcagttcagg gctttcttca gatgttccgc cagctcggca atggcctccg gcgaatcgtt
    11761 atcgataatg atgtgcttac gttctgtttg cggcggtacg cgaatgccga ccagttcagc
    11821 aagtggcgcg ctctggctcc agtcaatatc acttgcctgc cactgattta ccggtttttt
    11881 acccgcgccg agaatggctt tcatcgaagg aatgcgtggc acgttaatat cggaggtgac
    11941 gcagagcacg gctggaacag agagttcaat aacttcaaca tcatcttcaa gcgtgcgttc
    12001 aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg
    12061 cagaatttct ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcacc
    12121 aaagatcagt aaatcgaagc cgatcttttc aattgctgcc gccagcgctt ttgcggtatc
    12181 gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg
    12241 ggatagcacg tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc
    12301 ggctatctca tcgtcatctg ttgcgagctg gcttgcagct tcaatggcat tgagatcgaa
    12361 ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat
    12421 gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag
    12481 tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc
    12541 ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    12601 cgatcgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    12661 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    12721 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    12781 atgtatcaac gctgttttga taacgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    12841 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgttcat
    12901 gacaatgaaa ccgagttgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    12961 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    13021 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    13081 tttcaggggg ctgaggaaaa tcagctactg caaccgcatt cttgtccggt aattgccgcg
    13141 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    13201 agtaaaaata gccaaacatc ttcgttatgg gatgcatttg cctatacatt agcaattctt
    13261 tactacgaaa a
//
LOCUS       DAZFAH010000124        13233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFAH010000124 DAZFAH010000000
VERSION     DAZFAH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.124"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..289
                     /locus_tag="ACHJWB_003286"
     CDS             <1..289
                     /locus_tag="ACHJWB_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFO4314863.1"
                     /translation="IRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIA
                     LALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(431..757)
                     /gene="ypeC"
                     /locus_tag="ACHJWB_003287"
     CDS             complement(431..757)
                     /gene="ypeC"
                     /locus_tag="ACHJWB_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4314864.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(872..2128)
                     /locus_tag="ACHJWB_003288"
     CDS             complement(872..2128)
                     /locus_tag="ACHJWB_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4314865.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQL
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2332..3297
                     /gene="glk"
                     /locus_tag="ACHJWB_003289"
     CDS             2332..3297
                     /gene="glk"
                     /locus_tag="ACHJWB_003289"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4314866.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            3516..3842
                     /locus_tag="ACHJWB_003290"
     CDS             3516..3842
                     /locus_tag="ACHJWB_003290"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4314867.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3864..5111
                     /locus_tag="ACHJWB_003291"
     CDS             3864..5111
                     /locus_tag="ACHJWB_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4314868.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5126..6211
                     /gene="ypdF"
                     /locus_tag="ACHJWB_003292"
     CDS             5126..6211
                     /gene="ypdF"
                     /locus_tag="ACHJWB_003292"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4314869.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIATELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6211..7248
                     /gene="ypdE"
                     /locus_tag="ACHJWB_003293"
     CDS             6211..7248
                     /gene="ypdE"
                     /locus_tag="ACHJWB_003293"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4314870.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            7273..9768
                     /gene="ptsP"
                     /locus_tag="ACHJWB_003294"
     CDS             7273..9768
                     /gene="ptsP"
                     /locus_tag="ACHJWB_003294"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4314871.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGSGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTKHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9771..10628)
                     /locus_tag="ACHJWB_003295"
     CDS             complement(9771..10628)
                     /locus_tag="ACHJWB_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4314872.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSNYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10641..11375)
                     /gene="ypdB"
                     /locus_tag="ACHJWB_003296"
     CDS             complement(10641..11375)
                     /gene="ypdB"
                     /locus_tag="ACHJWB_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4314873.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11390..13087)
                     /gene="ypdA"
                     /locus_tag="ACHJWB_003297"
     CDS             complement(11390..13087)
                     /gene="ypdA"
                     /locus_tag="ACHJWB_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4314874.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3121 a         3387 c         3599 g         3126 t
ORIGIN      
        1 cattcgcttc catatcccgc tgtgggtgcg tcgtacagtc accatgttgc cgtcatttat
       61 tgtcattctg atgggattag atccgacacg gattctggtt atgagtcagg tgctgttaag
      121 ttttggtatc gccctggcgc tggttccact gctgattttc accagtgaca gcaagttgat
      181 gggcgatctg gtgaacagca aacgcgtaaa acagacaggc tgggtgattg tagtgctggt
      241 cgtggcgctg aatatctggt tgttggtggg gacggcgctg ggattgtagt tgaatgagcg
      301 tcgcatctgg cactattggc ggatgcggcg taaacgcctt atccgcccta cgcgttaaag
      361 agccggtttg tagatcctga taagacgcgc tagcgtcgca tcaggcattg tgttccattg
      421 gcgaatgtgt ttaatggcgg tgtccatgtc cgcggccttt tccgcgatga tcgtcacgat
      481 cgcgccagcc ttcacgatag ccacgctcat acgctttacg cttatcccag ccacgatgat
      541 agccattatc atgacgccac cagcggtttt tacgccactc ataattgcga tgccagtagt
      601 cacggtcgcg ccagtgacca ccgtcccagt aattaccgta atgatcgcga tcgccaattt
      661 gtaatttgat tgatggcagt agggtgattt cacctgcgtt tgctgcaagc ggggtaaatg
      721 ccatcagggc ggccgccaga aacagtgacc tgaacattgt tattctcctt cacgatcgaa
      781 gccgtcagcg gcctgttaac gcaatattac ggggaggtaa agccccgtat catcgccata
      841 actcttaaat cataaaggag agcatttttt gctaaacgtt atcgtggggt ggctgttgct
      901 tcggacgatt gaccatcatc atcggcttac ctgctaataa cagccatgcc gggagcatga
      961 cgatacagag cagcggtagc aatgtggtat tgggtacaac gactgccgcc ataaaaagac
     1021 ttaaccagcc atcgcgtgtt accaccagca cgatgccgag aatagcgcag gaaacggtta
     1081 ttgccgctgg aacggcggga acgtgctcat gcagcatcaa ccctaatgcc acgccgacaa
     1141 acactgccgg gaagattcgc ccaccgcgaa agccactggc ggcagcaacg accagggcgg
     1201 taagtttaat caccgccagc aaaaaataat cgctggtgct gaaagcctga tttgccacca
     1261 tctgctgcat ctcatccagc cctttaaaca gcgaaaccgg cccaccaata actcccagaa
     1321 taccgagaat aaatccgcca atacctagca cgagcaccgg atttttcatt tgatgcatca
     1381 tcgcgtgcaa ccgtggtaag caccatacgg cgaccattcc tgctgcgatg gcaatcgccg
     1441 cgacaattgc accgctgaga atatcggtca tttccaactg tccgtaatgc gcaatgggca
     1501 gtgaaaaatg gggatggaaa aataatccgg tagtaagtgc accagctgct gccgccatta
     1561 atggcgcaaa aagacgatcc catagcggaa cttcactact gccatttaag gtttgcgaaa
     1621 atatcaacgc cgccgcaaca ggtgtaccaa acagcgcgcc gatggttccg gcagaggcta
     1681 aaatagtcca ctccattcgg ttgacgcgcg gtaacagacg agcgccaatg gcaaccgcaa
     1741 gggcgatatt gacggtcatg atcggatgtt ccggccccag gctgacgccg ccagcaagac
     1801 cgagaattaa tgctacgata agtcctggta gcgcagaggg cggaactggt gcgccgatca
     1861 gcggttcaca ggcggggtct ggtccggcat gaccctggct gaaacggata accaacccca
     1921 ccgcaatacc cgttagcgtt aatacaccga tgatccagag aggtgaatcc tgggctatcc
     1981 ccagagttcc cggcagtcgt tgccagagca aattctgtaa taccgaggcg attttcatca
     2041 ccacaatcag aataagactg gacgcaatcc caattgccac ggcggggagc gataataaca
     2101 acatggttct ggctcgcgga tggagcatgt tctattcctt atgcggggtc agatacttag
     2161 tttgcccagc ttgcaaaaag gcatcgctgc aattggtgct gaaacgataa agtaaatgtg
     2221 tgacccagat cgatatttac agggagcctg cctttccggc gttgttgtta tgcccccagg
     2281 tatttacagt gtgagaaaga attattttga ctttagcgga gcagttgaag aatgacaaag
     2341 tatgcattag tcggtgatgt gggcggcacc aacgcacgtc ttgctctgtg tgatattgcc
     2401 agtggtgaaa tctcgcaggc taagacctat tcagggcttg attaccccag cctcgaagcg
     2461 gtcattcgcg tttatcttga agaacataag gtcgaggtga aagacggctg tattgccatc
     2521 gcttgcccaa ttaccggtga ctgggtggca atgaccaacc atacctgggc gttctcaatt
     2581 gccgaaatga aaaagaatct cggttttagc catctggaaa ttattaacga ttttaccgct
     2641 gtatcgatgg cgatcccgat gctgaaaaaa gagcatctga ttcagtttgg tggcgcagaa
     2701 ccggtcgaag gtaaacctat tgcggtttac ggtgccggaa cagggcttgg ggttgcgcat
     2761 ctggtccatg tcgataagcg ttgggtaagc ttgccgggcg aaggcggtca cgttgatttt
     2821 gcgccgaata gtgaagaaga ggccattatc ctcgaaatat tgcgtgcgga aattggtcat
     2881 gtttcggcgg aacgcgtgct ttctggtcct gggctggtga atttgtatcg cgcaattgtt
     2941 aaagccgaca accgcctgcc agaaaatctc aagccaaaag atattaccga acgcgcgctg
     3001 gctgacagct gcaccgattg ccgccgtgcg ttgtcgctgt tttgcgtcat tatgggccgt
     3061 tttggcggca atctggcatt aacgctggga acctttggtg gggtttatat tgctggcggt
     3121 atcgtgccgc gcttccttga gttcttcaaa gcctctggtt tccgtgccgc atttgaagat
     3181 aaagggcgct ttaaagaata tgtccatgat attccggtgt atctcatcgt ccataacaat
     3241 ccgggtcttc tcggttccgg tgcacattta cgccagacct taggtcacat tctgtaaatc
     3301 cttcctgtta tatcgggagg taactctccc gataatcttt taaatcatac agttaattca
     3361 atttttcttt gtgtcccctc acaaggccga cctgcgtcac acttccgtac agcgggatta
     3421 attctccagt aaatgcatta tttgtctggt aacggcgatt tgttttgcac gttcataatt
     3481 tcactcgtca actatcactg aatgaggaat taacgatgag taagaaactg attgccttat
     3541 gtgcctgccc aatgggcctg gctcacacct ttatggccgc tcaggcgttg gaagaagcgg
     3601 cggtagaagc cggttatgaa gtgaaaattg aaacccaggg cgcggacggt atccagaatc
     3661 gcctgacggc gcaggatatc gccgaagcaa ccatcatcat ccactccgtg gcagttaccc
     3721 cggaagataa tgagcgtttc gaatcacgcg acgtttatga aatcactttg caggacgcaa
     3781 ttaaaaacgc tgcgggcatc atcaaagaaa tcgaagagat gattgcctct gaacaacagt
     3841 aatattcgac agggataatt gttatggcca ttaaaaaacg cagtgcaacc gttgtgcctg
     3901 gcgcatccgg tgcggcagcg gcagttaaga atccgcaggc ctctaaaagc agcttctggg
     3961 gtgaactccc gcagcatgtg atgtcaggga tttcacgcat ggtgccaacc ttaattatgg
     4021 gcggtgtgat cctcgctttc agccagctga ttgcttatag ctggcttaaa attcctgctg
     4081 agatcggcat catggatgct cttaatagcg ggaaattctc cggtttcgac ctctccttac
     4141 ttaaatttgc ctggctgtcg cagtccttcg gcggcgtgtt gtttggtttt gccattccga
     4201 tgtttgctgc ttttgtggcg aactctatcg gtggcaaact ggcgttcccg gctggtttta
     4261 tcggcgggtt gatgtctacc cagccgacgc aactgctgaa cttcgatccc agcactatgc
     4321 aatgggcgac ctcttcgccg gtgccgtcaa ccttcattgg tgcgctgatt atttctatcg
     4381 tcgcgggtta ccttgtgaag tggatgaacc agaaaatcca gttaccggat ttcctgttag
     4441 cgttcaaaac cacatttttg ctaccgattc tttccgccat ttttgtcatg ctggcgatgt
     4501 actacgtcat cacccccttt ggtggctgga tcaacggcgg tatccgtact gtgctgactg
     4561 ccgcaggtga gaagggcgcg ctgatgtacg caatggggat cgccgcggcg acagcaatcg
     4621 accttggtgg cccgatcaac aaagccgcag gtttcgttgc cttcagcttt accactgacc
     4681 acgtattacc ggtcaccgct cgttctatcg ctatcgttat tcctccgatt ggtctgggtc
     4741 tggcgaccat tattgaccgt cgtttaaccg gcaaacgcct gttcaacgct caactttatc
     4801 cgcagggtaa gaccgcgatg ttccttgcct ttatggggat tagtgagggt gcgattccgt
     4861 ttgcgctgga aagccccatc accgccattc cgtcgtatat ggtcggcgcg attgtcggct
     4921 caaccgccgc tgtctggctg ggcgcagtgc aatggttccc ggaatccgct atctgggcat
     4981 ggccgctggt cactaacctg ggcgtctata tggcggggat cgcgctgggg gcagtcataa
     5041 ctgcgctgat ggtggtgttc ctgcgtctga tgatgttccg taaaggcaaa ttgttaatcg
     5101 atagcctgta agaaggaaaa tccagatgac attactcgct tcgctgcgcg actggcttaa
     5161 ggcgcaacaa ctggatgcag tgcttctctc ctcacggcag aacaaacagc cgcatctggg
     5221 gatctccacc ggatcaggct atgtggttat tagccgtgaa agtgcgcaca ttctggtaga
     5281 ttcgcgctat ttcgttgagg tggaagcccg tgcgcaaggc taccagctgc atttgcttga
     5341 cgcgacgaac acgcttacca ctatcgtcaa tcaaatcatt gccgatgagc agttgcaaac
     5401 gctcggtttt gaaggccagc aggtgagttg ggaaaccgcg caccgctgga agtctgaact
     5461 caatgcgaaa ctggttagcg ccacgccgga tgtgctgcgg caaatcaaaa cgccagagga
     5521 ggtggagata atccgcctcg cctgtgggat tgccgatcgc ggagcggagc atattcgccg
     5581 ctttattcag gcggggatga gcgagcgcga gatagccact gaactggagt ggtttatgcg
     5641 ccagcagggc gcagaaaaag cctcttttga caccattgtc gccagcggct ggcgcggggc
     5701 gctgccgcac ggcaaagcca gcgacaagat tgttgcagcg ggcgagtttg tcactctcga
     5761 ttttggtgcg ctgtatcagg gctactgctc tgatatgacg cgcaccttgc tggtgaatgg
     5821 cgaaggggtg agcgccgaat ctcacccgct gtttgacgtc tatcagattg tcctgcaggc
     5881 acagctcgcg gcaatctctg caattcgccc cggcgtgcgc tgccagcaga ttgacgacgc
     5941 cgcgcgccgg gtcattaccg aggctggatt tggcgactat ttcggtcata acactggtca
     6001 cgctatcggc attgaagtcc atgaagatcc gcgtttttca ccgcgggaca ccacgacgct
     6061 acagccaggc atgttgctga ccgtggagcc ggggatttat ttgccagggc aagggggcgt
     6121 gcgcatcgaa gatgttgtgc tggttacccc gcaaggcgca gaagtgctct acgccatgcc
     6181 gaaaacagtg ttgctcacgg gagaggcata atggatttat cgctattaaa agcgttgagc
     6241 gaggcagatg cgatcgcctc ctcggaacag gaagtgcggc agatcctgct ggaagaagcg
     6301 gatcgcctgc aaaaagaagt gcgatttgat ggtctgggat cggtgctgat ccgcctcaat
     6361 gaatcgacag gtccgaaggt gatgatctgc gcccatatgg acgaagtggg atttatggtg
     6421 cgcagcatct cccgcgaagg cgctattgac gtgctgccgg ttggcaacgt gcgcatggct
     6481 gcccgccagc tgcagccggt gcgcatcacc acccgtgaag agtgcaaaat tccaggcctg
     6541 cttgacggcg accggcaggg gaatgacgtc agcgccatgc gcgtggacat tggtgcgcgc
     6601 tcgtatgacg aagtgatgca ggcgggaatt cgtccaggcg atcgcgtcac ctttgataca
     6661 acttttcagg ttctccctca ccagcgggtg atggggaaag cctttgatga ccgcctcggt
     6721 tgctacctgc tggtgacgtt actgcgcgaa ctacacagcg ctgaactgcc tgcggaagtg
     6781 tggctggtgg ccagttccag cgaagaggtg gggttacgcg gcgggcaaac tgccacccgc
     6841 gcggtgtcgc cggatgtcgc cattgtgctt gataccgcct gctgggcgaa aaactttgat
     6901 tatggcgcgg ctaaccatcg tcagatttgt aacgggccga tgctggtgtt aagcgacaag
     6961 tcgctgattg cgccgccaaa acttaccgcg tggatcgaaa ccgtggcggc agaaattggc
     7021 gtgccgttac aggcagatat gttcagcaac ggcggcacgg acggcggagc ggtgcactta
     7081 accggcaccg gcgtgcctac agtggtgatg gggccagcaa cccgccacgg acattgcgcc
     7141 gcatcgattg ccgattgccg tgacattttg cagatgcagc agcttttatc tgcccttatt
     7201 aaacgtctta cgcgtgagac ggttgttcaa ctgacggatt tcagatgatc tcctgattaa
     7261 cccggagcgg ttatgttaac gattcaattt ctctgtcctt tgcctaacgg tctacatgct
     7321 cgtccggcgt gggaacttaa agaacagtgc agccagtggc aaagcgaaat cacctttatc
     7381 aaccatcgcc agaacgcaaa ggccgatgct aaaagctcgc tggcgttgat tggcagtggc
     7441 accttattta atgacagttg cagcctgaac attagcggca gcgatgaaga gcaggcgcgg
     7501 cgcgtgctgg aagagtacat cctggtgcgc tttatcgaca gcgacagcgt tcagccgacg
     7561 caggcagaac tgacggcgca tccgctgccg cgttcattaa gccgccttaa cccggattta
     7621 ctgtacggca atgtgctggc aagcggcgtc ggcgtgggta cgctgaccct gttacaaagc
     7681 gacagcctcg acagttatcg ggcaattccc gccagtgcgc aagattccac ccggctggag
     7741 cacagcctgg caacgcttgc cgagcaactg aaccagcaat tgcgtgagcg tgacggcgaa
     7801 agcaaaacta tcctcagcgc tcatttgtcg ctgattcagg atgatgaatt tgcaggcaat
     7861 atccgtcgcc tgatgacaga acagcatcag ggactggggg cggcgatcat cagcaatatg
     7921 gagcaggttt gcgccaaact atctgcttct gccagcgatt atctgcgcga gcgggtcagc
     7981 gacattcgcg atatcagcga acagttgctg catatcacct ggccggaact gaagccgcgc
     8041 aacaatctgg tgctggaaaa accgaccatt ctggtggctg aagatttaac cccaagccag
     8101 tttttgagcc ttgatttgaa aaatcttgcg ggcatgattc tggagaaaac cgggcgcacc
     8161 tcgcatacac tgattctggc ccgtgcctcg gcgatcccgg tactgagtgg cttgccgctg
     8221 gatgcgattg cccgttatgc cgggcaacct gcagtgcttg acgcccagtg cggcgtgctg
     8281 gcgattaacc cgaatgacgc ggtgagcggt tattatcagg tcgcacagac gctggcggat
     8341 aaacgccaaa aacaacaggc gcaggccgcc gcgcagctgg cctattcccg tgataacaag
     8401 cgtattgata ttgcggcgaa tatcggcacc gctctggaag cgccaggcgc gtttgccaac
     8461 ggcgcggaag gcgttggact gttccgtact gaaatgctct atatggatcg cgacagcgcg
     8521 ccggacgagc aggagcaatt tgaagcctac cagcaggtgc tactggcagc gggcgacaag
     8581 ccgattatct tccgcacaat ggacatcggt ggcgataaaa gcattcctta tctgaatatt
     8641 ccccaggaag agaacccgtt cctcggctat cgcgcggtac gtatttaccc ggaatttgct
     8701 ggcctgttcc gcactcaact acgggcgatt ttgcgcgccg ccagttttgg caacgcccag
     8761 ttgatgatcc cgatggtcca cagtctcgat cagatcttat gggtaaaggg cgagatccaa
     8821 aaagcgatcg ttgagcttaa gcgcgatggc ctgcgtcatg cagagacgat tacgctgggg
     8881 atcatggtgg aagtgccgtc ggtgtgctac atcatcgatc acttctgcga tgaggtcgat
     8941 ttcttcagta tcggctccaa cgatatgacc cagtatctgt atgcggtcga tcgtaataac
     9001 ccgcgcgtat cgccgctgta taacccgatt acgccatcgt tcctgcgcat gttgcagcag
     9061 attgttacca ctgcgcatca gcggggcaaa tgggtaggca tttgcggtga actgggcggt
     9121 gaaagccgtt atctgccgct actgcttggg ctgggcctgg atgagctgag tatgagtagc
     9181 ccgcgtattc cggcggtgaa aagccagctt cgtcaactgg atagcgaggc gtgtcgggaa
     9241 ctggcgcgtc aggcatgtga atgccgcagt gcgcaggaaa ttgaagcgtt actcaccgcc
     9301 tttacgccgg aagaagacgt tcgcccactg ctggcgctgg agaatatctt tgttgatcag
     9361 gattttagca ataaagagca ggcgatccag ttcctgtgcg gcaacctcgg cgttaacggg
     9421 cgcactaaac atccgtttga gctggaagaa gatgtctggc agcgggaaga gattgttacc
     9481 accggcgttg gttttggcgt agcgatcccg cacaccaaat ctcagtggat ccgtcattcc
     9541 agtatcagca ttgcccggct ggcgaaaccg attgactggc agtcagaaat gggcgaagtc
     9601 gaactggtga tcatgctgac gctgggtgct aacgaaggga tgaatcatgt gaaagtcttc
     9661 tcgcagctgg cgcgtaaact ggtgaataaa aacttccgcc agtcgttgtt tgccgcgcaa
     9721 gatgcacaaa gtatcctgac gctgctggaa acagaattaa ccttctgacg ttagccctga
     9781 aaacgggcgc tgtactctcc cggcgtcaga ccaaactgac gccggaaaac gcgacaaaaa
     9841 tagttgctat ccggaaaacc gcaacgctgt gccacttcat gaattgacag atgatatttc
     9901 tgcaaaatca tcctcgcctt cgccattcgc acccaacgca catactcgat aaaacgcatc
     9961 gttccatgct gagcaaacag ttttgacaga tgattgggcg tgatattaaa aaactgcgcc
    10021 acgctctcgc gggtgagcgg ctgggcataa ttatcctgaa cccagttaca aatgctgtga
    10081 tagagaaaat cgccgcgcgg ctgcgaattg cccggcggca tattcaccac gttacggcaa
    10141 agatgcagca aacttaacac cagtggctga atgatattct gctccagcgg cgaacggcta
    10201 agatgggtga gggcggtaag catcgcttca ccttcaccgc gttgcaggct gggaagctgg
    10261 atctggcgag caggatgcaa caacgacgtg gtgcggctat cgtaaaacga taacccaagc
    10321 caggtcggtg cgaacaccag gcttaacagc atcaccggtt tgttgttgat cggtaaatta
    10381 gccgcgcgtg ccggaataaa cagcatttct ccttccacca ttcgctgctg ctttgcttcc
    10441 agcaggttgc cgtattcgcc gcgcagcacg atatccagac ggggaaaatc aatacataaa
    10501 ctgcccaccg gtaacgaggc aggctggctg gcaaaccaga cgcgccccag tagttgcggg
    10561 ttaagcacca ggccgctgaa cagatcggcg aaaaattgtt gatcggcggg caagcctggt
    10621 gctttcatga aagttcccga ttaaagatgc attaactggc gaaattcttt cactttgctg
    10681 cggctgaccg gcacttcaaa atccagatct ttcagtcgca gaatgtaggt gttattaaac
    10741 cacggttcga tttcgcgtat tttgttcaga ttgacacaaa atgagcgatg gcagcggaaa
    10801 aaatgcgacg gcggcagttt gctgcaaaat tcggtaatgt tcatcggcat tacgtaggat
    10861 tcacgccgcg tatagacaaa ggtcattttc tcgtgcgctt cggcgtaata gatatcgtta
    10921 attggcgtga cgattattcg ctcatctttc accagattaa tggtgtcatt ctcacgcgtt
    10981 accgtcgcgg caggcgtact gctggtctgc tgttgttgcc aggccgcttc cagtttttgc
    11041 agcatcccgg taatacgtga ctcctgatac ggtttgagaa tgtagtcaaa cgcctccagt
    11101 tcaaacgctt ctaccgcatg ttctttccac gcggtgatga acacaataaa cggtttatgg
    11161 gcgaactggc tgatgttttg cgccagcaac acgccatcca gcgacggaat attgatatcc
    11221 agaaaaatgg cgtcgacgcg gttatgctgc aaaaacttca acacgtccag accgtcgtca
    11281 aaggtgccga caatctccat ctggctgtgc tctttaatta gccagctcag ttcctgttgt
    11341 gccaggaatt cgtcttcaac aatgatgact ttcacaatat cactccggct caaagcaata
    11401 acgtagcctg tgaggcgact ggggtgcgtt ggttaggaat gtaaaacgca atttccgttc
    11461 ctggttccag gcggcggata tgcagcccct cgccatacaa taacttcacg cgatgatgaa
    11521 cattcagcag gccaatttta ttgcccggca tttcattcgc ttcgacccgc tcaatcacct
    11581 ttggatcgat gccgtgcccg gtatcccgca ccgcaatgcg tacccgattg ccgcactctg
    11641 caacgctgat ggtgacaacg cctttacctt tgcaaggctg aataccgtgg acaatggcat
    11701 tctccaccaa cggctggatc agcaggctgg gaatgcagca attcacctct tcatcaatat
    11761 catagataac cgtcagcttg tcgccaaagc gggcctgctc aatggcaata tagtctttaa
    11821 tttgatacag ctcttttttg atatcgattt gctcatcgtc ttttaattca atgttatagc
    11881 gcagataacg cgacagatta aagatcagct ggcgagcagt atccggattc agacggattg
    11941 acgatgaaat agcgttcaaa gcgttaaaca ggaaatgggg attaattttg ctttgcaggg
    12001 cgcgaagctc tgccttattt gccatttcac gtagctgctc ggcgcgtgaa acctccagtt
    12061 gggtggagat gatttgcgac agaccgaccg ccatttcctg taatgacgag gtgatctgat
    12121 gcgcgtggca gtagtaaatt ttcagcgttc cggtcacaac gcctttctcc cacaatggga
    12181 tcaccagcat ggaatgaatc tctggtgtgc ggtgggcttc atcattgttt ttaatgatga
    12241 tttttccgta attcatcgcc tgacgggtag tcgggctaat gaagtcatcg ccattctgat
    12301 agttatgttc acccacgcca acataggcca gcacatgatc ggtattggta atcgccaccg
    12361 catcggcgtg aatgtcatcg cgaataattt cacaaacttt gcgtaatgac tcgctattga
    12421 catggcgaaa cagaggtagc gttttgttgg cgatatccag cgccagcttg gcctgccgcg
    12481 cggcgctggc ctctttttcg ccctcaacgc tttgcaccag aagcacaata aagccgatac
    12541 agacgctacc gaggatcatt ggaatgccga ttttagagac gatatcgatc cccaacgcgg
    12601 tagttggtgc ccagacaatt accagaatca tggtcaacgt ctcacacaac atgccgccga
    12661 gaataccgac gcgccagcgc tgtgctttgg ggattttcag gtttatccag cctgatatac
    12721 aacccgccag aatgctggtg ataaagcagg ggatcgccgt cacgccgccg atatcaatta
    12781 aataccggtg aatacccgca atcacgccgg taatgatgcc tacccacggg ccaaacagaa
    12841 tcccgccgga catcaccgcg ataatacgta cgttcaccag cgagccttct acgggaacgc
    12901 cggaccaggt gctgaacagg gcgaacagcg agaaaatggc ggtaacggcg agcaattctt
    12961 ttggggagtg cgccgacttg tgcaacagtt cgcgaaacag gcggatacgg atgagaaaga
    13021 acaggcagat aagcattaac gctgcccgat cgaagaccgc cagcagcatg ttgaatattt
    13081 cgtgcacggg tgactctgaa taaggattaa acctctatga taggtaacct gaaggctgat
    13141 gaccagcagg ccgtttttga ggaaaaacca taaatttaaa aggggattga aagataaatg
    13201 gtgtattagc aatgtatctc ttgataatgc act
//
LOCUS       DAZFAH010000125        13228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFAH010000125 DAZFAH010000000
VERSION     DAZFAH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.125"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..292
                     /locus_tag="ACHJWB_003298"
     CDS             <1..292
                     /locus_tag="ACHJWB_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4314875.1"
                     /translation="QWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGG
                     ERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
     gene            complement(553..4776)
                     /gene="rpoC"
                     /locus_tag="ACHJWB_003299"
     CDS             complement(553..4776)
                     /gene="rpoC"
                     /locus_tag="ACHJWB_003299"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4314876.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(4853..8881)
                     /gene="rpoB"
                     /locus_tag="ACHJWB_003300"
     CDS             complement(4853..8881)
                     /gene="rpoB"
                     /locus_tag="ACHJWB_003300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4314877.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(9201..9566)
                     /gene="rplL"
                     /locus_tag="ACHJWB_003301"
     CDS             complement(9201..9566)
                     /gene="rplL"
                     /locus_tag="ACHJWB_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4314878.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(9633..10130)
                     /gene="rplJ"
                     /locus_tag="ACHJWB_003302"
     CDS             complement(9633..10130)
                     /gene="rplJ"
                     /locus_tag="ACHJWB_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4314879.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(10543..11247)
                     /gene="rplA"
                     /locus_tag="ACHJWB_003303"
     CDS             complement(10543..11247)
                     /gene="rplA"
                     /locus_tag="ACHJWB_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4314880.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(11251..11679)
                     /gene="rplK"
                     /locus_tag="ACHJWB_003304"
     CDS             complement(11251..11679)
                     /gene="rplK"
                     /locus_tag="ACHJWB_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4314881.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(11838..12383)
                     /gene="nusG"
                     /locus_tag="ACHJWB_003305"
     CDS             complement(11838..12383)
                     /gene="nusG"
                     /locus_tag="ACHJWB_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4314882.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(12385..12768)
                     /gene="secE"
                     /locus_tag="ACHJWB_003306"
     CDS             complement(12385..12768)
                     /gene="secE"
                     /locus_tag="ACHJWB_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4314883.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(12998..>13228)
                     /gene="tuf"
                     /locus_tag="ACHJWB_003307"
     CDS             complement(12998..>13228)
                     /gene="tuf"
                     /locus_tag="ACHJWB_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4314884.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
BASE COUNT         3056 a         3609 c         3280 g         3283 t
ORIGIN      
        1 ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg acaacaacat
       61 cgctgaaaat gcccttcggg cggtcagtct gggtcgtaaa aacttcctgt tcttcggctc
      121 tgaccatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt gcaaactgaa
      181 tgacgtggat ccagaaagct accttcgcca tgtgcttggc gtcatagcag actggccggt
      241 caaccgggtc agcgaactgc ttccgtggcg catagcactg ccagctgaat aacacatccc
      301 cgtcaatacg gccctcgctg tacgcttact cctgtttcat tctcgttcct taagaaagtt
      361 acacaggaat agtatcgaat ccttgccgag tttgactcaa ttagttcagt cagtttcagg
      421 atattagtca tctctacatt gattatgagt attcagaaat tccttaaata ttctgacaaa
      481 tgctctttcc ctaaactccc cccataaaaa aacccgccga agcgggtttt tacgttattt
      541 gcggattaac gattactcgt tatcagaacc gcccagacct gcgttcagca gttctgccag
      601 gctggcagat gcgtcttctg cagtcacctg cggtgcagcc ggagcttcac ccgcagcacg
      661 gcgacgcata cgatcctggt ggtacgcgta accggtacct gccgggatca gacgacccac
      721 gataacgttc tctttcaggc cgcgcagttc gtcgcgtttg cccgcaacgg ctgcttcggt
      781 cagcacgcga gtggtctcct ggaacgatgc cgcggagatg aaggactcgg ttgccagaga
      841 cgctttggtg atacccagca gatcgcggga gtaagttgca cccactttgc cgttcgcttc
      901 cagttcgcgg tttgcgatct tgacgcgaga gtattcaacc tgttcgcctt ccaggaagtc
      961 ggagctgccc gcgttaacga tggtagcttt acgcagcatc tgacgaacga taacttcgat
     1021 gtgtttatcg ttaatcttaa cgccctgcag acggtatacg tcctgtactt cgttaacgat
     1081 gtaacgagtc acagcatgaa caccacgcag acgcagaatg tcgtgcggcg cttccggacc
     1141 gtcggaaatt acgtcaccac gttctacacg ttcaccttcg aacacgttga gctgacgcca
     1201 tttcggaatc atctcttcgt acggatcgct accgtctacc ggggtgataa ccagacgacg
     1261 tttacctttg gtttctttac cgaaggaaac gataccgctg atttcagcca ggattgccgg
     1321 ctctttcgga cgacgagctt cgaacaggtc cgcaacgcgc ggcagaccac cggtgatgtc
     1381 cttggtaccg ccggattcct gcggaatacg cgccagggtg tcaccagagc tgatctgtac
     1441 gccatcttcc agctgaacaa tcgctttacc cggcaggaag tactgcgcag gcatatcggt
     1501 acctgggatc agaacgtcgt taccctgagc atcaacgatt ttcagtgccg gacgcagatc
     1561 tttaccacct gcggtacgtt ctgcggaatc cagaaccacc agcgaagaca gaccggtcaa
     1621 ttcgtctgtc tgacgagtaa tggtctgacc gtcgatcatg tcagtaaagc gtacaaaacc
     1681 gcttacttcg gtgataaccg gcatggtgtg cgggtcccag tttgcaacgg tttcgccgcc
     1741 agcaacctgt tcgccatcgc ctttcgccag taccgcaccg taaggtactt tgtagctttc
     1801 tttggtacga ccgaattcgt cgatcagttt cagttcagta ttacgggaag tgataaccag
     1861 tttaccgctg gagttcacaa ccgacttcac gttgctgagc ttgatgctac ctttgttttt
     1921 cacctggatg ctggattcag cagccgcacg agatgccgca ccaccgatgt ggaacgtacg
     1981 catggtcagc tgtgtacccg gttcaccgat ggactgtgcc gcgataacac cgattgcttc
     2041 acccttgttg atgatgtggc cacgcgccag gtcacgaccg tagcagtgcg cacatacacc
     2101 aaagtcggtg tcacaagata caacagaacg tactttaacc gcgtcgacag agttctcttc
     2161 cagcaggtca caccactgtt cgtgcagcag cgtgttgcgc ggaacgagga tatcagcagt
     2221 acccggcttc agaacgtctt cagcagttac acgacccagt acgcgatcgc gcagcggctc
     2281 tttaacatca ccaccttcga taaccggagt catcatgata ccttcatggg taccacaatc
     2341 gtcttcggta accaccaggt cctgcgccac gtcaaccaga cgacgagtca ggtaaccgga
     2401 gtttgcagtt ttcagtgcgg tatccgccag acctttacga gcaccgtggg tggagatgaa
     2461 gtactggagt acgttcagac cttcacggaa gttcgcggtg attggcgttt cgatgatgga
     2521 gccatccggc ttcgccatca gaccacgcat accagcaagc tgacgaatct gtgccgcaga
     2581 accacgcgca ccggagtcgg ccatcatgta gatgctgttg aaggaaacct gcttctcttc
     2641 ctgaccgtca cggttaatca cggtttcagt ttgcaggtta tccatcatcg ctttggatac
     2701 acgatcgttc gccgcagccc agatatcgat aactttgttg tagcgttcgc ccgcagttac
     2761 cagaccagac tggaactgct cctgaatttc agcaacttct gcttctgcct cggagatgat
     2821 ttcgtgtttc ttctccggga tgaccatgtc atcgatacca acagatgcac cagaacgcgc
     2881 tgcataggcg aagccggtgt acatgatctg gtccgcaaaa ataacggtcg gtttcagacc
     2941 gagaatgcgg tagcaggtgt tcagcatttt ggagattgct tttttaccca gcgcctggtt
     3001 gacgatggag taaggcagac ctttcggtac aatcatccac agaatggcac ggccaacagt
     3061 cgtgtctttc aggctggttt tcgctactaa ttcaccgttt gcatcttttt catactcggt
     3121 gatacgcact ttaacgcgcg catgcagaga agccagacca gagcgataca gacgttctgc
     3181 ttctttcggg ccagtcagca ccatgccttc gcctttggcg ttaacacagt cacgggtcat
     3241 atagtacaga cccagaacaa cgtcctgaga cggaacgatg attggttcgc cgttcgccgg
     3301 ggacaggatg ttgttggtag acatcatcag cgcacgcgct tccagctggg cttccagcgt
     3361 cagcggtacg tgaacagcca tctggtcacc atcgaagtcg gcgttatatg ccgcacaaac
     3421 cagcgggtgc agctggatag ctttaccttc gatcagtacc ggttcaaatg cctggatacc
     3481 cagacggtgc agagtcggtg cacggttcag cagtaccggg tgttcgcgga taacttcgtc
     3541 caggatatcc caaacgacag cttcttcgcg ctcaaccatt ttcttcgcag ctttaatggt
     3601 ggtagcaaga ccacgcagtt ccagcttgcc gtagatgaac ggtttgaaca gctccagcgc
     3661 cattttcttc ggcagaccgc actgatgcag acgcaggtat ggacctacgg tgattacaga
     3721 acgaccggaa tagtcaacac gcttaccgag caggttctga cggaaacgac cctgtttacc
     3781 tttgatcatg tcggccaaag atttcagagg acgcttgtta gaaccggtga tcgcacgacc
     3841 gcgacgaccg ttatccagca gggcgtctac cgcttcctgc agcatacgtt tttcgttacg
     3901 tacgatgatg tccggcgcag ccagatccag cagacgtttc agacggttgt tacggttaat
     3961 gacgcgacga tacagatcgt tcaggtcaga agtcgcgaaa cgaccaccat ccagcggaac
     4021 cagcggacgc agatctggcg gcagtaccgg cagaacggtc aggatcatcc actctggttt
     4081 gttaccagac tgaacgaacg cttccagcag tttgatacgc ttggtcagct ttttacgctt
     4141 ggtttcggag ttggtttcgt tcagctcttc acgcagctgt tcgcactctt gctccagatc
     4201 catgctcttc agcagagcct ggattgcttc cgcccccatc ttcgcgtcga attcgtcacc
     4261 gaactcttcc agcgcgtcca gatactgctc ttcagtcagg atctgctgac gttccaggtt
     4321 ggtcataccg ccttcgataa ccacatagga ttcaaagtac agtacgcgtt cgatatcgcg
     4381 cagcggcata tcgagcagca gaccgatacg ggacggcagc gatttcagga accagatgtg
     4441 cgcagtcggg gaagccagtt cgatgtggcc catacgctca cggcgtactt tagtctgggt
     4501 cacttcaacg ccgcacttct cacagatgac gccacggtgt ttcaggcgct tgtacttacc
     4561 gcacaggcac tcgtaatctt ttaccggccc aaagatacgg gcgcagaaaa ggccgtcacg
     4621 ttctggtttg aacgtacggt agttgatggt ttccggcttt ttaacttcac cgaaagacca
     4681 tgaacggatc atgtctggcg aagccagagc aattttgatc gcatcaaact cttcggtttt
     4741 agtctgcgct ttcagaaact ttaataaatc tttcacggat ttgctcccgt cggagttagc
     4801 acaatccgct gccgggtttt aacccgacag cagtgacctg tttgagcgag aattactcgt
     4861 cttccagttc gatgttgata cccagcgaac gaatctcttt caacaatacg ttgaaggatt
     4921 ctggcatgcc cggctccatc tgatggttgc cgtccacgat gtttttatac atcttggtac
     4981 gaccgttcac gtcatcagac ttaacggtga gcatttcctg cagggtgtat gctgcgccgt
     5041 atgcttccag cgcccacact tccatctccc cgaaacgctg accaccgaac tgtgccttac
     5101 cacccagcgg ctgctgagta accaggctgt aagaaccggt ggaacgcgcg tgcatcttgt
     5161 cgtcgaccag gtggttcagt ttcagcatgt acatgtaacc aacggttacc ggacgctcga
     5221 actgttcacc agtgcgacca tcgtacaggc ggatctgacc ggaagtcggc aggtcgccaa
     5281 gtttcagcag ctctttaatt tctgcttctt tcgcaccgtc gaacaccggc gttgcgattg
     5341 gcataccttt gcgcaggttt tcagccagac gcataacttc ttcatcgctg aaggtactca
     5401 ggtcaacttt ctgacgaacg tcagcgccca gatcgtacgc acgctggatg aattcgcgca
     5461 gtttcgcgac ttcttgctgc tgtttcagca tggcgttgat cttgtcgccg atacctttcg
     5521 cagccatacc caggtgggtt tcgaggatct gaccgatgtt catacgagac ggtacgccca
     5581 gcgggttcag tacgatgtct accggcgtac cgttttcatc gtaaggcata tcttcgatcg
     5641 ggttgatctt agaaattaca cccttgttac cgtgacgacc tgccatcttg tcaccaggct
     5701 ggatacggcg tttaaccgcc agatatacct taacaatctt cagcacgccc ggtgccagat
     5761 cgtcgccctg ggtgattttg cggcgtttcg cttcgagttt cttctcgaac tcgtgtttca
     5821 gttcgtcata ctgctcagcc agctgttcca gctgattttg tttctcttcg tctgtcaggc
     5881 ccagctccag ccagcgatcg cgcggcagtt tgtcgagctt ctcagcttca acgccaccgg
     5941 ctaccagcac agcacggata cggctgaaca gacccgcttc gaggatctgc agttcttcag
     6001 acaggtcttt cttcgcctgt ttgagctgca tttcttcgat ttccagcgca cgtttgtctt
     6061 tttctacgcc atcgcgagta aagacctgaa cgtcgataac cgtaccggat acaccgtttg
     6121 gtacgcgcag agaagagtct ttaacgtcag aggctttctc accgaagatc gcacgcagca
     6181 gtttttcttc tggggtcagc tgagtttcac ctttcggcgt taccttacca accagaatgt
     6241 cgccaccggt cacttccgca ccaatgtaaa cgataccgga ttcatccagt ttggagagcg
     6301 cagcttcacc cacgttcggg atgtcagcgg tgatctcttc cggccccagc ttggtgtcac
     6361 gggacacaca cgccagttcc tgaatgtgga tggtggtgaa acggtcttcc tgaacaacac
     6421 gctcggatac gaggatggag tcttcgaagt tgtaaccatt ccacggcatg aacgctacgc
     6481 gcatgttctg accaagcgcc agttcaccga ggtcggtgga cggaccgtct gccagcacgt
     6541 cgccacgttc aaccggttca cccagagaca cacacggcat ctggttgata caggtgttct
     6601 ggttagaacg ggtgtatttg gtcaggttgt agatgtcgat acctgcttca cccggataca
     6661 tctcgtcttc gttaactttg ataacgatac gggaagcatc cacgtactga acgacaccac
     6721 cacgtttagc taccgcagtt acaccggagt caacggcaac agcacgttcc ataccagtac
     6781 caaccagcgg cttatcagcg cgcagagtcg gaacggcctg acgttgcatg ttcgcaccca
     6841 tcaatgcacg gttggcgtca tcgtgttcca ggaacgggat cagggacgca ccgacggata
     6901 ccacctgctg ggtggatacg tccatgtagt caacctggtc gcggctgaac aagctggatt
     6961 cgcctttgct acggcaagtt accaggtctt ctacgaagtg gccttcttca tccaggttgg
     7021 agttcgcctg ggcgataacg tagttgcctt cttcgatagc agacaggtag tgaatttcgt
     7081 cagttacaac accgtcggtc actttacgat acggagtctc aaggaagccg tattcgttag
     7141 tctgtgcgta cacggacaga gagttgatca gaccgatgtt cggaccttca ggggtttcga
     7201 ttggacatac gcgaccgtag tgagtcgggt gtacgtctcg aacttcgaag cctgcacgtt
     7261 cacgggtcag accgcctggg ccgagtgcgg agatacgacg tttgtgcgta atctcagaca
     7321 gcgggttgtt ctggtccata aactgagaca gctggctgga accgaagaac tctttcactg
     7381 ctgcggaaat cggcttggcg ttgatcatat cctgaggcat cagggtatcc agatcgccca
     7441 gagacagacg ctctttcacc gcacgctcta cacgtaccag gccaacgcgg aactggtttt
     7501 ccgccatttc gccaacggaa cggatacgac ggttgccgag gtggtcgata tcatcgactt
     7561 cgcctttacc gttacggata tcgatgagct ttttcataac atcaatgatg tcgtctttgc
     7621 tcaggatacc ggaaccttcg atttcttcgc gcagcagaga acggttgaac ttcatacgac
     7681 caaccgcaga caagtcataa cggtcttcgg agaagaacag gttctcgaac aggctttcag
     7741 ctgcttcacg agtcggcggc tcgccagggc gcatcatgcg gtagatttct accagtgcgc
     7801 tcagacggtc gttagttggg tcgacacgta aggtttcaga gatatatggg ccgtgatcca
     7861 gatcgttggt gaacagcgtt tcgatacgct tgtgaccaga ctggctcagc ttagccagca
     7921 gatccaggct cagctccatg ttcgctgcgc agatcagctc gccggtagac tcatcaatat
     7981 agtctttagc aaccacttta cctgcgatgt actcaaccgg gacttcgatc agtttgacgt
     8041 cgtctttttc cagctggcga atgtggcgcg cagtgatacg gcggcctttt tctacgtaca
     8101 ctttaccgtt agcttcgatg tcaaaagatg cggtttcacc acgcaggcgt tccggcacca
     8161 gttccatctg cagcttgtta tcacggattt caaagataac tttttcaaag aacaggtcga
     8221 ggatctgctc tgtggtgtag ttcagggcgc gcagaatgat ggtcgcaggc agtttacggc
     8281 gacggtcgat acgtacgaac aggttgtcct tcggatcgaa ttcgaagtcc agccaggaac
     8341 cacggtaagg gatgatgcgc gcgttataca gcactttacc cgaagagtgg gttttacctt
     8401 tgtcggagtc aaagaagacg cccggactac ggtgcagctg ggaaacgata acacgctcag
     8461 taccgttgat aacaaaggta ccgttgtctg tcatgagcgg aatttcgccc atgtagactt
     8521 cttgttcttt aatgtctttt acggtgcctt ccggcgcttc gcgctcatag atcaccagac
     8581 gcagtttaac gcgcagcggt gcggaatagg tcacgccacg gatttgacat tcctggacgt
     8641 caaacaccgg ttcgccaagg cggtagctga cgtattgcag ctcggaatta ccgctgtagc
     8701 tctgaatcgg gaatacggaa cggaaagcag cttccagacc atactgccct tcaggatctt
     8761 gctcgataaa tttctgaaac gagtcaagct ggatagaaag gagataaggt acatccagaa
     8821 cttgtggacg tttaccaaaa tccttacgaa tacgtttttt ctcggtatag gagtaaacca
     8881 tagggttcct cagctcgctg acaagtcgac ccatctgtcc attgagcgga cagtttgtgc
     8941 aacactattt tgttgaccgg aaaatggaac actttccgca atgcctgttg ctatcacgct
     9001 taaaccattt cattgcgatt tacacagaac ggacgtcctg tcgcagtata ttaagtcgtc
     9061 gatagaaaca agcattgaaa ggcacagcag tagtcaaaca gtgtgaaacg ctactggcgc
     9121 cttacagcgc aaaaaggctg gtgactaaaa agtcaccagc catcagcctg atttctcagg
     9181 ctgcaaccgg aagggttggc ttatttaact tcaacttcag cgccagcttc ttccagagct
     9241 tttttcagtg cttctgcgtc gtctttgctc acgccttctt tcagagcagc cggtgcagat
     9301 tctaccaggt ctttagcttc tttcagaccc aggccagttg cgccacgtac tgctttgata
     9361 acagcaactt tgttagcgcc agcagctttc agaattacgt cgaattcagt tttttcttca
     9421 gcagcttcaa ccgggccagc agctacagct acagcagcag cagcggaaac accgaatttt
     9481 tcttccattg cagagatcag ttctacaacg tccattacag acatagctgc aactgcttca
     9541 atgatttgat ctttagtgat agacatttaa attgttcctg aatatcagaa taagtttata
     9601 cgtaagcgaa tgcgttaaaa agataactgc gattaagcag cttctttcgc atcgcgtaca
     9661 gcagccagag tacgaaccag tttgccagcc gaagcttctt tcatggttgc catcaggcgt
     9721 gcaattgctt cttcgtaggt cggcagagtt gccaggcggt cgatctgaga cgccgggatc
     9781 agctcacctt caaaggcagc ggctttgacc tcaaattttg cattcgcttt cgcgaactct
     9841 ttgaacagac gagcagcagc gcccgggtgt tccatagagt atgcaatcag ggtcggacca
     9901 acaaacgcgt ctttcaggca ctcgaacgga gtaccttcaa cagcacggcg cagcagggtg
     9961 ttacgaacaa cacgcatgta tacgccagct tcgcgacctg ctttacgcag ttcagtcatt
    10021 ttatctacag ttacgccacg ggaatccgca actactgcag acagcgcgcc tttggctact
    10081 tcgctgactt cagcaacaat cgcttgtttg tcttgaagat ttaaagccat tagctttgct
    10141 cctggatgtt tgccagagaa aatctctgga actcacttca ctcctccaaa cggagagagc
    10201 gtcttaatta cggtgagcag aaacaagcca gaatataaaa gaataatctt agcgttctgt
    10261 caccgtctac gcaggggatt aagtttcttg cgaaactcct gcggtcttcg acggaggcct
    10321 ggataggcca ggctccaacg aacaaatctt ttctatccgt cgctattcaa cttgtggcgg
    10381 cgttggcttc cttcattcgc accagtcaca tagttagcta tgctcctggc gactcactca
    10441 gttgccgcct tgccacaacc cgaatatctc ggatatacgt ctgcattaag cattatacgt
    10501 gggggtaaga ttgtagacaa aatcaccgcc cacgtaaagg cattagttta cagaagcgct
    10561 caggccagcc tggtcaactg caacacctgc acccatggtg gtggagatgc taactttctt
    10621 gatgtacacg cctttcgcct gagtcggttt tgcttttttc agcgcaacca gcagagcttc
    10681 caggttttct ttcagtttgt cagcgtcaaa gtccacttta ccgatggtgg tgtggatgat
    10741 gccgtttttg tcgttacggt aacgaacctg gccagcttta gcgtttttaa ccgcttcagc
    10801 aacgttcggt gttacagtac ccactttcgg gtttggcatc aggccgcgcg gacccagaac
    10861 ctggcccagc tggccaacaa cgcgcattgc atccggagaa gcaataacaa cgtcaaagtt
    10921 catttcgcct ttcttgatct ggtcagccag atcttccata cctaccagtt ctgcgcctgc
    10981 agctttagca gcttcagcgt ttgcaccttg ggtaaatacg gctacgcgaa cggaacggcc
    11041 agtaccgtgc ggcagtacag ttgcaccacg tacgttctgg tcagatttac gagcgtcgat
    11101 gccgaggtta acagctacgt ccacgctttc tacgaattta gcagtcgcca gctctttcag
    11161 cagtgcgata gcttcgttga tgtcgtactg tttggttgca tcaactttct cgcggataac
    11221 acgcatgcgc ttggtcagtt tagccatttc ttagtcctcc actaccaggc ccatggaacg
    11281 tgcagtacct tcgatggagc gagtcatcgc ttcaatgtcg gcaccagtca tgtcggcagc
    11341 tttggtctgc gcgatttcct gcagctgagc gcgggaaatt ttacccactt tgtctttgtt
    11401 cggcttaccg gaaccagact tgataccagc cgcttttttc agcagaactg ctgccggcgg
    11461 agtcttggta acgaaagtga aagaacggtc agcgtaaacg gtgattacta ccggaatcgg
    11521 cagacctttt tcgatggaat cagtttttgc gttgaacgct ttgcagaatt ccatgatgtt
    11581 tacgccctgc tgacccagag ccggacctac tggcggactc gggttagcca taccagctgc
    11641 aacctgcagc ttgacatagg cttgtacttt cttagccatt ataaattcct caagttgggt
    11701 aataacgcct ctgagaggct ccccgtgata taaaaaatcg ttttacgggc aaggcccata
    11761 aaaacaaaag gcgcgaaatt gtattccaat ctcacgcctt gtgcaacgat taaatcgccg
    11821 cttttttgat cgctgggtta ggctttttca acctggctga agtccagctc taccggagtc
    11881 gcacgaccga agatagaaac agacactttc agacgagatt tctcgtaatc cacttcttca
    11941 acaacaccgt tgaagtcagc gaacggacca tcattaacac ggaccatttc acccggttca
    12001 aacagcgttt tcggacgcgg cttatcacca acctgctgca ggcggttcat aatcgcatcg
    12061 acttctttat cgctgattgg cgcaggacga tcggaagtac cgccgatgaa gcccatcaca
    12121 cgcggtacgc tgcgcaccag gtgccagctc gcgtcgttca tcaccatctg aacgaggacg
    12181 tagccaggga agaatttacg ttcgcttttg cgacgctgac cgccacggat ttcaaccact
    12241 tcttcggttg gtaccatgac ttcaccaaac aaatcttcca tgttgtgtaa tttgatatgc
    12301 tcacgcagcg acgttgctac gcggccttca aaaccggaaa acgcctgaac gacgtaccag
    12361 cgctttttag gagcttcaga catctcagaa cctcaggcca gtgataaagg ataccaggcg
    12421 aaccagaata ccatccagtc cccacaggat cagtgacatt actgcggtaa ccgcagccac
    12481 aatcagcgtg gtgtgcaatg tttcctggcg agtcggccaa atgaccttac ggacttcggt
    12541 acgcgcttca cgggcaaaag caacggtagc tttacctttt gtcgttaaca gcgcgacacc
    12601 acccgctgca gcaatcagaa ttactacggc cagcgcacgc agcggcagca taatgtcgcg
    12661 ataaaggtag ttgccgacaa tcgccaccag gagcaatgcc accacaacga cccacttcat
    12721 cgcttccagg ccgcgcccgc ttccttgagc ttcggtattc gcactcataa accaacctgt
    12781 cagaagtttt ctacaaacat tttcaccccg cgatcgcgag gcaaaccaaa tcgaaacgcg
    12841 tattagcttt tcggattata cgccctcaac agagcctgtc tcagcaatga ttatgacaaa
    12901 gaaaatcact gatgagccag gttctggtac gaaagcgtgc aaaaagggca tcaaatgatg
    12961 cccttttagt gcgcattgcg tcaaatgtta tcggcaatta gctcagaact tttgctacaa
    13021 cgcccgcgcc aacggtacgg ccgccttcac ggattgcgaa acgcagaccg tcgtccatcg
    13081 cgatcgggtg gatcagggta acaaccattt tgatgttgtc gcccggcatt accatctcta
    13141 cgccttccgg cagttcgatg gtaccagtca cgtcagtagt acggaagtag aactgcggac
    13201 ggtagccttt gaagaacgga gtatgacg
//
LOCUS       DAZFAH010000126        13202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFAH010000126 DAZFAH010000000
VERSION     DAZFAH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.126"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..1181
                     /locus_tag="ACHJWB_003308"
     CDS             123..1181
                     /locus_tag="ACHJWB_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4314885.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1191..2333
                     /locus_tag="ACHJWB_003309"
     CDS             1191..2333
                     /locus_tag="ACHJWB_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4314886.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2326..3099
                     /locus_tag="ACHJWB_003310"
     CDS             2326..3099
                     /locus_tag="ACHJWB_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4314887.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3101..4180
                     /locus_tag="ACHJWB_003311"
     CDS             3101..4180
                     /locus_tag="ACHJWB_003311"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4314888.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4180..5136
                     /locus_tag="ACHJWB_003312"
     CDS             4180..5136
                     /locus_tag="ACHJWB_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4314889.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5147..6355
                     /locus_tag="ACHJWB_003313"
     CDS             5147..6355
                     /locus_tag="ACHJWB_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO4314890.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6373..6840
                     /gene="terW"
                     /locus_tag="ACHJWB_003314"
     CDS             6373..6840
                     /gene="terW"
                     /locus_tag="ACHJWB_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4314891.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7101..7430
                     /locus_tag="ACHJWB_003315"
     CDS             7101..7430
                     /locus_tag="ACHJWB_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4314892.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7417..7797
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003316"
     CDS             7417..7797
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4314893.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7840..8670
                     /locus_tag="ACHJWB_003317"
                     /pseudo
     CDS             7840..8670
                     /locus_tag="ACHJWB_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8701..10314)
                     /locus_tag="ACHJWB_003318"
     CDS             complement(8701..10314)
                     /locus_tag="ACHJWB_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4314894.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10345..10695)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003319"
     CDS             complement(10345..10695)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4314895.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10692..11054)
                     /locus_tag="ACHJWB_003320"
     CDS             complement(10692..11054)
                     /locus_tag="ACHJWB_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4314896.1"
                     /translation="MVKLASQPGASVARIAREHDINDNLLFKWLRLWQNEGRISRRLQ
                     VTTSSDTGVELLPVEITPDEQKEPVAALTPLLSTPSQSTVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            11171..11922
                     /locus_tag="ACHJWB_003321"
                     /pseudo
     CDS             11171..11922
                     /locus_tag="ACHJWB_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3155 a         3479 c         3458 g         3110 t
ORIGIN      
        1 acactgacgc actccataac cacaggagtt attaatcata ttatgattga catgtagctt
       61 agacactcat agactcactt ctcaatcata aatttgcatg gcgttccgga taattaaaaa
      121 aaatgaacaa gaaaatttgg tttatggaag gtttatcctc ccagcgagat attattcagg
      181 gggtaaaatc atttgcacaa aaaaataatt ttgccattac cgtttttgcc tcccaccgta
      241 acgaaagaaa tgaaatcctt tccgttgccg attattcttt gactgaacct gaagatcctc
      301 aaaaacgtct tcagtttatc caggaaacca ttcagaccta cggcatccac catattcata
      361 ctggccgtaa cagccagtgg tttgaagaac accgttcagc cattgaaccg accggtgcca
      421 cccttactac cggtgcaacg ggcgtcgact ggttaactct ggctgacgaa aaagttactt
      481 ttgctcagtt tatggagcaa aagggtctcc cggtcgtacc atcctggcgg gtgaatacgc
      541 tggcagaatt aaagacacac ctcgcggccc cgccgttcac tgacagcccg gtatgcgtga
      601 agccggtgac gggtatctat ggcatgggat tctggcgctt tgatgacagt gcttcgccta
      661 tggccgtctt taatcatccc gaacatcgtc tggtcagtcc gcaacagtat attgcagcag
      721 catcagctgc tgagtcgttt aaaccccttg ttttgatgcc gtacctgcca ggcccggaat
      781 tttccgtcga tatcctcgcg gataagggcg aaatactcgc agccgtggga cgccgtaagg
      841 aaggggctat ccagtatctg gtaaacgaag gaagcgcctg ggaactggcg tgtgactgcg
      901 cccgtgttat gaaggccgac gggctggtga atgttcagac gcgaaacgat gtgaatggca
      961 acccggtgct gcttgaaacc aacatgcgtc cgtcaggggg ggtgggttat acccttcaca
     1021 gcggtgtgaa ccttcctggg ttatttgctg cctttaagct cggtctgatg tctgaagata
     1081 tggtacgcca gagcgctaaa aacacctttt ctccggttgc ggtgagatcc attacggatg
     1141 taattgcata cccggaatca ctctctaacc ttctgaatta aggcgtattt atgtctgata
     1201 tcctcgaaga tatgatttat cgccgtaccc tctcatgcgg tacgattcag gtaacccgtg
     1261 accagggtga agtttcgctc gatgacctgt ttgacatcgc tgaaagacgt aacccgaaac
     1321 gcgcctttct gtttgtcagc aaagtactcg gcaggcacat tcccgtgcca ccctcagtca
     1381 tgcgacaggc ctacagacag cttgccagcc agttcccctc gacactgaca ggacccgtac
     1441 tgtttatcgg tatggctgaa accgccgttg ggcttggggc cggtgtattt gatgaagtgc
     1501 gccaccagca tcgtgaatct gtctatctga cctctacccg tcacccggtt gatggcacgt
     1561 tgctttgcga gttcaaggaa gaacacagcc acgccaccga tcatctgatc tatctgccag
     1621 atgatgaaga gaaaagacgt cgtgttacca acgcacgaac gctggttttg attgatgacg
     1681 aggcaaccac cggtaatacc tttattaacc tgctttctgc cctgcgtaat accggcaagc
     1741 ttcaacatat tgaacaggtt atcgccgtaa cgctgaccga ctggagtggc aatgccctgt
     1801 cggagcgcac ccccttgccc gtcacttcgg tttcgcttgt aagcggtcag tggggatgga
     1861 ccccattacc tgatgcacct gtcccggaca tgccgaaagt caacgtaact tcacgaggag
     1921 aatgggacat ccaggggaaa cagtcatggg gccgactggg gatgctcgca cctgcggccg
     1981 atctcggcca tgaggtctcc gtccacaagg gggaacgtat tctggttctc gggaccgggg
     2041 aattcgtctg ggagccgttc ctgcttgctg aacggctcga agctgccgga gcacaggcat
     2101 tttatggatc gaccacccgc tcccctgtcg ccgttggtta tgccattgag tccgccattt
     2161 cctttacgga taactacggg ctgggcatcc ccaattttgt ctataacgtc gcccaccagc
     2221 agtttgaccg cattcttgtg tgtactgaga cacccgcaga aagtattgac acgcagcttc
     2281 ttaaagcgct ggctgaggtt gcgcccgtcg tggagattgt tacctatgaa taaacccgtt
     2341 gttctgagcg accttgacga cacgctcttt cagacccgtc gtaaaatggt ggacgagctg
     2401 gccctggagc cattccgtac cggtgccgtt gatcgcaccc tgaatccacg aagctttatg
     2461 acggaagaac agtccatgtt ggtggactgg ctcctggagc aggctgaact catacccgtt
     2521 accgcacggg gaactgaaga aatcagccgc gtacggatcc ctttccactc ctgggcaatc
     2581 acaacgcacg gggccgtcat tctcacgcct gaaggcaaac ccgacgagga gtggaaggcc
     2641 catatgctcg gccagctggc tccctaccag gaaaagctga catcgatgca gcgtctgatc
     2701 actgaaatga tggacgcaaa aggaatcaat gcctgggcaa ggctgaactt cgaatacggt
     2761 gaaacggcgg tttatatggt gatgaaacac cgcgacagca cccgccttga cgagctcaat
     2821 gccattgcag atgagataga aacggtgttt ccgaccgagg gcttctacat ccaccgcaac
     2881 agtaataacg tggcctggct tcccaccccg gttgagaaag ggctggcagt cagctggctg
     2941 cttgaaaaac ttcgggctga acgaggagtt ttccccgtaa ttggtttggg cgacagcctg
     3001 agcgatcatc gttttatgaa actgtgcagc tggtttggca tcccgcgtca gagtcagttt
     3061 gcagatgcca tttcacagcg aatttttgga gaaaattaaa atgcagccac atgaaacatt
     3121 taccggctca taccagcccg gtgacgtgga atttctgcta aagccggttg tcattgagat
     3181 gacgccggtt gagaaaaaag aagaactgat tcagtccggg aagaaacatt attcggacat
     3241 gctcagccag gagccagcgc ctacacaatg gcatcttgat ttgtttcacc gggcgctgga
     3301 tcggggcgca gagaggcttg caaaagaagt cacacagctc gctattgctc tggccgaacg
     3361 cttcggtgat gagcccattg tactggccag tcttgtcagg gctggcgttc cgctcggcgt
     3421 tatgctgcac caggccctgc gtgacatggg gaaaacctca tggcattacg gtatcagcat
     3481 tatccgggat cgtggaattg acggtgcagc cctcgacgtc attgaagagc ggcatggtac
     3541 cagcggtatt gtctttgttg acggatggac aggtaaaggc gcaattaccg gagagcttgt
     3601 acgcgcactg aaagatcgcc cgggctatcc tgagcagccg cgactggttg tcctggccga
     3661 tccctgtggc tgctcctggc ttgcagccag tgatgatgac tggctcattc cttttggcat
     3721 catgggggcg ccggtatcag gcctgatctc ccggtcagta tggtcctctg aaggattaca
     3781 cgggtgcatg gtttgcgagc atctcagtga attcgaatgc agccggatgc ttgccgatac
     3841 cgtcgctcat ttccgtaaga agttaacacc gtcatccctc gctcccctga gctggaatac
     3901 ggaatcagcc cgggtcttat ggcagacaag tcgcgacgtt atcgcgttcc tggccgatga
     3961 atttaaagtg gacagcgtca atcgtattaa acccggtatt gctgaagcaa cccgggctgt
     4021 attacgtcgg gtaccggacc atgtatttgt gcgttctatt gacgacccgg acgttgcctt
     4081 gcttgtaggg cttgctcgtg aaaagggaat agttgttaca gaaatggggg gaaccctcgg
     4141 ccagtatcgg gctgtaacca ttatcaagaa ggtactctga tgaaaaatcg tctttctccc
     4201 tggaatctgg gagccacgct atacatgcct gcaacacggg aagatattgc tgatgccgtc
     4261 ctgcacggga aaatcccggg cttgcgctct ctggtgattt gcctggagga tgctgtcagt
     4321 gaagcagaca tccccgtcgc cctcaaaaat ctggagcacc tgctgcacga gctcagtaac
     4381 agcatgagta gcctgggtaa aaatgactgg cctctggtgt tcattcgccc ccggcatgcc
     4441 gaaatgggca gatggttaaa agcgcattat gatctttccg ctgtcgacgg gtttgtgctg
     4501 cctaaattta ccctcagttc gttagctgaa tggtgggata tcatggtcgg gactcacctg
     4561 tgcatgatgc caacgctgga aacagaagat gtctttgacg tggttcagat gcgcgagctg
     4621 gctacccgtc tggaggaaca tccctgccac gaccgcatta ttgcgcttcg aatcggcggc
     4681 aacgatctta tgaacgttgt gtcgcttcgc cgtccccggg acctgacgct gtatgacagc
     4741 ccgatgggct acgtcatcaa aatgctcgtt tcggtcttcg gcccgcgtga ttttgccctg
     4801 accgcgcctg tatgtgagca tattgacgat catgccgtta tggccagaga gctggctctt
     4861 gatatggcac acgggcttgt tgggaaaacg gccattcacc cgggtcagat agaggtcatt
     4921 caaaacgcgc tgatggtcac tcagggtgag cattctgacg ctctgcggat cctgaactct
     4981 acccaggccg tgtttaagtc gcagggagca atgtgtgaac ccgccacaca tcgccgctgg
     5041 gcggctggca ttctggacag ggctcgtttt tatgggttac agaacgagca aagcgctgat
     5101 ggaatcagat tacttaccgt gacccagcat cattaaggaa atcataatgg ggttcaggtt
     5161 cagaaagtcg atcaacatta ttcctggcgt tcgcctcaac ctgagtaacg gtgcaccgag
     5221 cctgagtgtc gggccgagag gtgcttccgt ttcttttggt agccggggga cctatgccaa
     5281 tctgggcttg cccggtaccg ggctgagtta ccgtacccgg cttgaccggg ccgcgcgttc
     5341 cagaggtgaa aaccggacgg caaccgaccc ggggctcaga caggcgcttg agcagaaagc
     5401 cgctgaactc atgtcagcgg taaccgcaat ccgtaatatt cacgagctga cgccggatcc
     5461 aaaaacaggc atcagctggg cagagctgga agcagtatac ctgcataaca gaacgtcgcc
     5521 ttttcaggtt ccggcaccgg tgcgtccaga aaagccagac taccttgtat tgccggaaaa
     5581 gcctgccgag agcgaaggca ttagtttttt gggtaaatgg tttgaatcgg aatcagctaa
     5641 agctgagcgc cacgccgaaa atcttcgccg gtggcagcag gagctgattg atgtggagcg
     5701 tgagaatacc cttcgacagc accggtacca gcaacaacgg acggcctggg ccgaacagta
     5761 tgcaaactgg aagtttgaag ctgaagaaca tgaaaaacgg ctcgccacgg ctcaggcaga
     5821 tgcccggcag cagttccgga cagacgccgc gtttttcgaa tcatacctgg cgggtgtgct
     5881 ggcagaaact gaatggccgc gtgaaacgct tgttgcattt gaagtaaagc cggagctatc
     5941 agcagtcctg ctggacgttg atttagctga gattgaagat ttccctgata agatttacgg
     6001 cgttaatgcc cggggaacgg agctgacgga aaaagccatg acgcaaaaag ccgtacgcga
     6061 aaactatgcc caccacgtcc atggctgctt gttccgcctg gtcggtatcg ttttacatac
     6121 gctacctttc gacaacgtga ttgtgtcagg ctttacgcaa cgggtcagta agcggaccgg
     6181 ctatctggag gatgagtata tcctgtcctg caaatgcact cgcagccaga tgtcgtcagt
     6241 aaattttgca ggcataaaac acattgatcc ggttgaagcg ttaggcgatc aaccggttat
     6301 tcgaaagatg agcagtacct tcatttttca gcctattgaa ccactaaccc tttaagcagg
     6361 ctaaatctta cgatgcaatt aaataccaga caggcccgga tttttaagct tgccaactta
     6421 ctgggcaccg gcaagcccgt ttccgctgcc gacattataa ccagtctgga atgctctgag
     6481 ccgacattaa cccgggccct caaagagcta cgcgagtcat attctgcgga gatcaaatac
     6541 agcaaggcag gtcattccta tcatctcgtt aatccgggcc agttggataa aaagaccctc
     6601 cgccggatga atgaagcgct cgcacagaat gctgaactga aaaccggtga gtccacaggg
     6661 aaggtcgtac tcgataagga taaaaaaaca gccgtgtcat tatcgctacg catgcggatt
     6721 ctaagaaaaa ttgaccgtct ggctgcgctg agtgactcga cccgaagtga agcggtagag
     6781 aagctggctc tgcactccgt tgatgaactg ataaaagaat acagcgctaa aaagtcctga
     6841 cgctaaatct cagaccgggg tgaaaactcc ggttttgtca tatttcacct ctgtattggc
     6901 gtgatgcaaa atggcatctg aatcgatcat ggtttggcat ccatttccga tcacagtttg
     6961 gcatcacaat cgatcacgat ttggcatgct tccgctcatt gattggcatc ctggtgtcgt
     7021 tacggggaag tcatcttcac agggatcttc agtcagttag cattaccgac atccattcac
     7081 caacttaccg gagataatca atgtcaaaac cccgatggac gcttgatcag aaaaaacatc
     7141 acgttgccgc atggcgcgcc agcggcctta cccgtgaaca gtactgtgaa ctttacgaca
     7201 ttccgtttaa atccctgcgc cagtggccgc aggatgtcgc aaaagccgaa aaacgagcca
     7261 gagcaccaga aattattcct gtttcagtat ctggctcttc cggaatgact gatggcagac
     7321 cattgtcgga tgagcctgtc actctcttcc tgcccggtgg tatccggatg tgctgccagc
     7381 cttctcagtt aaccgatgtt ttccgggctc ttcgccatgc tgacgcctga taacgtattc
     7441 ctggtcgtaa aacccgtcga tatgcgccgg ggtatcgaca cactgacaca gtacgtccag
     7501 aacgaactta acgctgcatg gcatgacggt gctgcttttg tcttcaccaa taaagtccgc
     7561 tcccgcataa aagttctccg gtgggacaaa catggcgtat ggctgtgtac ccgacgactg
     7621 caccggggaa gcttccgctg gccccggaaa ggtgatgcca catggcatct cacacaggat
     7681 gagtttcact ggctggtctt tggggttgac tggcaacagg tgaaagggca tgacctggca
     7741 aaatgggtat accaggaaga acccgaagat gctcagagca acgcaataac tcaatgaaat
     7801 ataatgaata tccatgcgtg atgtcatata atccaccgca tggatatctc gcttctcagt
     7861 accaccagtg accctgaaca gcttcgcgct ctggcaattg ccatggtgca aaaagtcatg
     7921 gctgagaatg cagaacttca gaaccgtatc cgcatcctgg aagagcagat gaagctcgca
     7981 cgccagcagc gtttcggtaa aaaatgcgaa tcgctggccg ggatgcagcg ttccctgttc
     8041 gaagaggatg ttgacgcgga tatcgcagaa atctcggcgc atctggacaa actgcttcct
     8101 cagaccgggg acgaagaaaa aacaacaaca cgtcctgttc gtaagccctt gccttcacct
     8161 cttccccgtg ctgaaaaggt tatcccgcca gctgaggaac gttgtcctga ctgtgatgcc
     8221 cccctgcact tcattcgcga tgaagtcagc gagaagctgg aatatattcc ggcacaggtc
     8281 gtggtcaacc gctatatccg cccgcagtac agctgtccct gctgtgagaa agtgttcagc
     8341 ggaaaaatgc cggcacatat ccttccgaaa agtgctgttg agccatcggt catcgcacag
     8401 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
     8461 cgaatggggg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgctggcgct
     8521 gcactggccc cgctggcaaa attgttacgc cacgagcttc tgacacggga tgttattcat
     8581 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
     8641 tggctgtggg cttacgtcag cggagaaaga gtaagcgtca acggagcacc gtattgacgc
     8701 ttatttattg gtgagtacta cgttccatgg caggagttca tcaactcggt tggaaggcca
     8761 ttctggcagt acgctcagga tatggcgcag atacgcttcc ggatcgatac cgttcagacg
     8821 gcaggtgccg atcagcccgt acaacagtgc tccacgctcg ccgccgtgat cgctgccaaa
     8881 gaacataaag tttttctttc cgagacagac tgcacgaagc gctctttccg cagcattatt
     8941 atccgcctcc gccagaccgt catcactgta ataacagagg gcatcccact gattcagtac
     9001 atagctgcac gcttcgccca gtctggattt tttcgacagc gtaccattct tctccaccat
     9061 ccattcatgc agtgacgtca gtaacacttt gcttcgctgc tgcctgacgg caagacgctc
     9121 tgactccggt aatccccgta tttcatcctc gatggcgtac agttcactga ttcgcttcag
     9181 ggcttcttct gccgtcgcac ttttgctgct gatgtataca tcgtggattt ttcgccgggc
     9241 atgggcccag cacgcaactt ctgtcagtgc accaccttca cgttctgcac tgaacagccg
     9301 atcgtaaccg ctgaacgcat ccgcctgcag gataccccgg aagggacgaa ggtgttgtac
     9361 cggatgtttt ccctgcctgt ctggtgagta ggcgaaccag accgccggtg gctctgatga
     9421 gcccgcattc cggtcatccc ggacatacgt ccagatgcgt cctgtttttg ccttttttct
     9481 gcccggtgcc agcactttta ctggtgtgtc gtcagtgtga accttgcggg tgttcatcac
     9541 gtaacggtac agggtatcat tcagcagagt cattaactga caacatgcgt caacccagtt
     9601 ggagagtaat gcccggctca gttcgatgcc ctggcgggca aagatttcac tctgacgata
     9661 cagtggcagg tgttcgcagt attttcccgt taacacgcgg gcaagtaatc cggagcccgc
     9721 gatgccgcgc tctatcgggc gggacggcgc tggcgcttca actatacagt cacattttgt
     9781 acaggctttt tttacccgaa cagtgcggat cactttcagg gcgctactca ccagttccag
     9841 ctgctcagca ctgacttccc ccagataatc cagctcactg ccacactccg ggcaacaact
     9901 ttcttcaggc tccagacggt gtatttcacg gggaagatgt gccggtaacg gacgacgatg
     9961 acgtgactgt cgcaactggc ggggaacctg cgggtcatcc tcacgcccac tgtaacgatc
    10021 gctttcctgt tcgcgttgtt tcagttgggc ctcagcctgt tcaacctcac gctgcagttt
    10081 ttcagaacgg gtaccgaaca acatccggcg cagtttttct atctgggccc tcagatgttc
    10141 tatttcccgc tcctcctctt cgatcttttc ctcggcacgt gccagtgcag agcgcaggaa
    10201 ggcctccgtc tcttcaacca gactcagttg ctgatctttc tgacggaggg cttcagcctg
    10261 ctcagagagc agcctttcca gctcagtgat acgaatgagg tatttccgac tcatgaccgt
    10321 ttttataatc cggccatgac atttttacaa cattgtcagt gcattaaggc gggatgtttt
    10381 gggctgacgc cagtccagtt tatcgaggag cattgccagc tgcgagcggg taatggatac
    10441 cttaccgtca cgcaccgcag gccagataaa ctggccttcc tccagacgtt tggtgaacag
    10501 gcacagacca tcagcatcag cccacagaat tttaatcgtg tcaccccgtc ggccgcgaaa
    10561 gataaacagg tgaccggaga aggggttctc atccagcaca tgttgtacct gttctcccag
    10621 accgttgaag gatttacgca tatcagtaac gccggcaacc agccagattc gagtgcctga
    10681 tgggagcgag atcatcgtcc cctcccggtc agttcacgga tcaacaccgt gagcagctct
    10741 ggtgaaggat tttccagcgt catgttaccg tggcggaact caactttaca ggaactggca
    10801 ctgactgtgc tttgtgaagg agtggataaa agcggagtaa gagccgccac cggttctttc
    10861 tgctcatccg gcgttatttc tacaggtaat aattcaacgc cagtgtcaga agaggtcgtt
    10921 acctgaagac gccgtgatat acgcccttcg ttctgccaga gcctgagcca tttgaacagg
    10981 aggttatcat tgatatcgtg ttcccgggca atacgggcaa cagaggctcc tggttgtgat
    11041 gccagtttaa ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgaggat
    11101 aatattttct gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca
    11161 atactggctt aatggctaca tacggcggtc agtttacgct tacgagaaag aagcggtccg
    11221 ccggtcgtct gttttgactc gcagaccgga cgggccctca gatatcctga ggcctggctg
    11281 cagggctggt gtggcggtac gctggtgagt gacggataca gcgtgtacaa atctctggcg
    11341 gataatgacc ccggtattac atctgcctgt tgctggagcc acgcaaggcg cggtttcgca
    11401 aatctgtata aggccagcag ggagccccgt gcagccatgg cgctgagaaa aatcgcgggt
    11461 ctttatcgca ttgagaagtt catccgtgaa cgtcctgtgg aaaaaatacg gcaatggcga
    11521 caacggtatt cccggccgat agtgaatgac ctgtttgcct ggcctgaaga gcaggagccg
    11581 tgctgtccgc ccgacggacc actaaacaaa gcgattaatt atatcctgaa ccggcgcgat
    11641 gaactgagct gcttcctgag tgacggagcc gtgcccctgg acaacaatat ctgcgagcgt
    11701 gctatccgcc cggtggtcat ggggcggaaa gcatggttat tcgccggttc actgatggcc
    11761 gggaaccgcg cggcacagat aatgagcctt ctggaaacgg caaaacgtaa tggtctggag
    11821 ccgcatgcct ggctgacaga cgttctgacg cgtctaccgg agtggccgga agaccgactg
    11881 gaagagttgc tgccccttga gggatttacc ttctccgggt gagtgatacc tgccatcagg
    11941 tgttcgtgca gcgggccata acccgcagtc gggagttgaa cgcctgacga caggaaatga
    12001 gccagagcag cgaccacgcg ggctgacagt gagtcagaac ctgaatcagc ccggagccat
    12061 tcccggggct gtacttcatc acctgccatc agtacccgtg tcagtacttc gcgacctgac
    12121 gcaattaccc gactcagttc ttcagaaccc gcctccgata ccagcctggc ggtcgccccg
    12181 gcgcaacaga ataaatcaac catcgtcaga ctgcgatcat tcgtatgcat cgctgatgat
    12241 gcgcccgaaa aacgcgtcaa tatgagttcg ccggaatatt actgacgaca cggtttgtag
    12301 ttacgttcct tcggtgagtg agtacctgtg cgtcagggta acgcaatcag ttcaggttta
    12361 gcggggtgtc aagtcggttt ggctaaccga ccgcagaatt aactgtacct ttaaaggtcg
    12421 cccccgatat atctgcctgt cagacatatc gccgattcgg tatctcacta cataaaactc
    12481 cttaaaaaac aatgcgattc tgttggtacg aagctttcag taagtcagtc cggagaagcg
    12541 ccagtacgat acagaatgag gttgaaaaaa acgttcacgt ctggtcactt cttcaaatgt
    12601 taaaaaacat ctcgtaaaga gccagtttat gcgggttcgt tgagtagacg tgcgagcgtc
    12661 actttgtcag ctttaacgct gacgaaagtg acgcccgcac agcttcaaac tccacatgca
    12721 cacgcaagcg accaaatttc aaatggtctt atgccgtgcg cacagtcata agaagcgcat
    12781 gcggaaagtc agaaaatatc ttaagacgca gattttaaag gcgtttttat gccagtctgc
    12841 cgggaataca cagtagtccg gttgtccgga agcaccacgg taatccgaag gaaaaatcaa
    12901 cgtacctcaa tcaccgtagt gatatccgtt cccagaggct aaacggatgt accaccataa
    12961 aggcatcctc tttcaggcta cgaggatatt gcaatcaccc gaaggcgttt ctctcagatt
    13021 gctgaaacat tggctggcag tcacccgaag gcgtccctct cagactgctg gaacattggc
    13081 tggtcgcaaa agcgacagtt aatgcaaata caggttaaca aaaagggaaa tctgccgtca
    13141 acccaaccat atgaacatgt tgatttcatt atgaatgtga tgttctaata taaattaatc
    13201 ag
//
LOCUS       DAZFAH010000127        13151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFAH010000127 DAZFAH010000000
VERSION     DAZFAH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.127"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..357)
                     /gene="cspH"
                     /locus_tag="ACHJWB_003322"
     CDS             complement(145..357)
                     /gene="cspH"
                     /locus_tag="ACHJWB_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4314897.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            643..855
                     /gene="cspG"
                     /locus_tag="ACHJWB_003323"
     CDS             643..855
                     /gene="cspG"
                     /locus_tag="ACHJWB_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4314898.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            866..1054
                     /gene="ymcF"
                     /locus_tag="ACHJWB_003324"
     CDS             866..1054
                     /gene="ymcF"
                     /locus_tag="ACHJWB_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4314899.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            1029..1259
                     /gene="ymcE"
                     /locus_tag="ACHJWB_003325"
     CDS             1029..1259
                     /gene="ymcE"
                     /locus_tag="ACHJWB_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4314900.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1249..1422
                     /gene="gnsA"
                     /locus_tag="ACHJWB_003326"
     CDS             1249..1422
                     /gene="gnsA"
                     /locus_tag="ACHJWB_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4314901.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1471..2544)
                     /gene="yccM"
                     /locus_tag="ACHJWB_003327"
     CDS             complement(1471..2544)
                     /gene="yccM"
                     /locus_tag="ACHJWB_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4314902.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2616..5360)
                     /gene="torS"
                     /locus_tag="ACHJWB_003328"
     CDS             complement(2616..5360)
                     /gene="torS"
                     /locus_tag="ACHJWB_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4314903.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            5443..6471
                     /gene="torT"
                     /locus_tag="ACHJWB_003329"
     CDS             5443..6471
                     /gene="torT"
                     /locus_tag="ACHJWB_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4314904.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6444..7136)
                     /gene="torR"
                     /locus_tag="ACHJWB_003330"
     CDS             complement(6444..7136)
                     /gene="torR"
                     /locus_tag="ACHJWB_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4314905.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7266..8438
                     /gene="torC"
                     /locus_tag="ACHJWB_003331"
     CDS             7266..8438
                     /gene="torC"
                     /locus_tag="ACHJWB_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4314906.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKDASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8438..10993
                     /gene="torA"
                     /locus_tag="ACHJWB_003332"
     CDS             8438..10993
                     /gene="torA"
                     /locus_tag="ACHJWB_003332"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4314907.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKSWPR"
     gene            10981..11580
                     /gene="torD"
                     /locus_tag="ACHJWB_003333"
     CDS             10981..11580
                     /gene="torD"
                     /locus_tag="ACHJWB_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4314908.1"
                     /translation="MATLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11673..11978)
                     /gene="cbpM"
                     /locus_tag="ACHJWB_003334"
     CDS             complement(11673..11978)
                     /gene="cbpM"
                     /locus_tag="ACHJWB_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4314909.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11978..12898)
                     /gene="cbpA"
                     /locus_tag="ACHJWB_003335"
     CDS             complement(11978..12898)
                     /gene="cbpA"
                     /locus_tag="ACHJWB_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4314910.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3200 a         3334 c         3441 g         3176 t
ORIGIN      
        1 atggcattta taactattag gtgcagagat attcgcttaa atggattagt ttctttgtgg
       61 aaaagtaact gatgttataa tgtatgatgt tgactattgc actattccaa taaaaccagt
      121 cagctttaaa caagcagcgt catattaaga gagataaaca tttgccgctg ttggtcctcg
      181 caggccattt acgcggcaaa attccacacg taatcctggt ataagcactt ctgcgtcgcg
      241 gggagtgaat gcggaaatat ggacctgaac ttctttacga ccgtcggagg ggataatgaa
      301 tcctttgccg cttttgcgat caaaggtttt gacaattcct gtcattttac gggacaaaca
      361 aattccttac tgaaaatact gcgctgcact atacggggtt aataaaataa agccagcgat
      421 atttaagacc gccggacggc taaaataaaa tttgcttaat ctcaattatc atgcgttaat
      481 agctgcgtcg gtttgaaaga cagacagcat acaaagtagt ttactaaagc agttctcatt
      541 atcaggcatt atccccttct tttgagtctc tctcctgaac actaagtagt ttctgtatta
      601 aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa atatgtctaa taaaatgact
      661 ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc tgatgatggc
      721 agcaaagacg ttttcgtcca tttcaccgcc atccagagca atgaattccg cacgctgaac
      781 gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggccccgc ggcagcgaac
      841 gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct gtccatgttg
      901 tcatggttca cagtaccgca catcggcatt cgatgtgacg gagcgaaacc ctttgggcgc
      961 taagtgtatt ttttgtaaat cgacgatgat cacctttgat aacgtcgcgc tgcaaatacg
     1021 cactgaccat gtgccgctgg atttcacaaa ataatatcag gctccctcgt ggagcctttt
     1081 ttatatctgc cttatttttc ttcaacgctg tatgtatagt aagcgataac ctgttgatta
     1141 ttgaatcttt cggggagatg gcttataaca tttcttacct gaccagggta ccgggaacca
     1201 acaccttact ggcgtgttgc tgtcttttaa gaccagaaga ggttaacagt gaatattgaa
     1261 gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa aatcgccgag
     1321 ctgaacaaga atacagggaa agaagtctct gaaattcgct tcaccgcacg agaaaaaatg
     1381 accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga acatcacccc
     1441 gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg cttgtggaat
     1501 taagcgtcgg tacaattcct ccggcaccgg gctttgccat actcccgcat acattgcgta
     1561 accaatcacc gcgaacataa tccccagaac cagtagcgtc attaaccagc cagacaacgc
     1621 aaaagctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg aggctaccgg
     1681 acatgactct acgcaagtca tacagccggt acattccact gttcgtacct gaattaattt
     1741 atctaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagccga tacaactttc
     1801 ggcattgcga cgaatcttaa acggcgataa tagcgaaacc acgcccatca gcgcgccata
     1861 tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca gcaaaacggt
     1921 cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt ttacgtccat
     1981 caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca tcaataacgc
     2041 gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa tatccagcca
     2101 gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga ttaattcaga
     2161 aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata gactgatgac
     2221 gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact gacctgttgt
     2281 aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga tacctcccgg
     2341 tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat accagaacgt
     2401 gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac gccaggtcgt
     2461 ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc gccgctgcca
     2521 gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat accaaatgcc
     2581 actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag gtctttctta
     2641 ttcaaccatg cttccagatc ggcaacactg cgtgtaattt cttcgtgtgg aaggggggca
     2701 gataatggct gctgctccag ttgtgcgcat agctggctgg cgatacgcat tcccaaactc
     2761 gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc ctgactggcg
     2821 cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa taccagccat
     2881 tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga tacatccagc
     2941 gattgatcgt tattgacttg cagttggaga tagtgcgcca gtaactgacc gagcacttca
     3001 cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg gcgtagcgtt
     3061 tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg ctgtgccagt
     3121 tgtcgggcaa gcgtaatgcc gtcgacatcc ggcagatcaa aatccaccag tgcggcagca
     3181 aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac agcaacaacc
     3241 tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg gttatcttca
     3301 attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt tggcacgggt
     3361 gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc cggcgtgctg
     3421 gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt cagccccagc
     3481 ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc tgccagtttc
     3541 gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc gccatcagtg
     3601 cgactacgca ggataatgta cccttcgtca gtaaaacgca gggcgttgct caacaggttg
     3661 gttataacct gacgaatacg tcgtggatcg cccattaacg cgcacggcat atcatcggca
     3721 attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa ttgcagggta
     3781 ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ccgaaacatt cttgccacct
     3841 gcttcgatag cggaataatc gagaatatcg ttgaggatgg tcagcaacga ttcaccagag
     3901 tcagtaattg cccgcaaatc atcacgctgg gcgttaagtg cggggttatc tgccagcagt
     3961 tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat cgccgccaga
     4021 aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg gtgttctatc
     4081 accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg ttcgcggtgg
     4141 cgattcagtg catgaacatt gctgcgaaac gcatccatca gccgcccgat ggtatccagc
     4201 tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag cagccgttgc
     4261 agcgcctgcg tttgttcggc aagtggacgc gtgactgagc gataaaccac gcgccaaagg
     4321 atcagaatca gtgcgcaaag tgaaaccatc cccagcaata acaggctgta ttgcccgcgt
     4381 gcactggctt tttccagatg cgccagtccg tgctgattac gcagctcaat ggtgtcgacc
     4441 agctgactga cttcgctact aaactgcgcg aactgggcga tgttattttg tgcgagagtt
     4501 tgtaggtgat tgctgatttc actgtcctgc tgatacagcg ccagcaaatc gctatattgg
     4561 ctaacggtag ttaacgttgt tgcgacctgc gcacgaacac ccggatcttc aatgcgtatt
     4621 tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt tggtgcattt
     4681 ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag agcgctaagg
     4741 cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg atcgagtgca
     4801 ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc ctgggtcgct
     4861 ccagcggaag ttgtcgcatt attcgcctga ccttgcgcca ggcgtgcgat ctcatcggcg
     4921 gcagcgacta tctgctgact gagttgccgt tgttgctggc gtagttgcag acgccgcccc
     4981 accagttccc cttgctgacg taatgaacgg gagatctcct gctcctgttg ttcaatagcg
     5041 gtggtatcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt caggctttgt
     5101 gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact ggtcaggttc
     5161 tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat attcatggta
     5221 ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag gttgtaccat
     5281 cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat ccagagtctt
     5341 cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga gtcgcaaagc
     5401 ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt actgctattt
     5461 ttacttcttt cccttttcat gttgccggca ttttcggctg ataacctgtt gcgctggcat
     5521 gatgcgcagc atttcacggt gcaagcctct atgccgctta aagccaaacg cgcatggaaa
     5581 ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa ctatggtatg
     5641 caggaggcag ctcgccgtta tggtgtggat ttaaaagtgc tggaggcagg cggctacagc
     5701 cagttggcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc agaggccatt
     5761 ttactcggta gtagcacgac ctcatttccc gatctgcaaa agcaggtagc aagtctgccg
     5821 gtgatcgaac tggtaaatgc tattgatgct ccccaggtga aaagccgcgt tggtgtaccc
     5881 tggtttcaga tgggctatca acctgggcga tatttggtgc aatgggcgca cggtaaacca
     5941 ctgaacgtgc tgttgatgcc cggacccgat aatgccgggg gcagtaaaga gatggtcgag
     6001 ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc gcttggtgat
     6061 aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca cccagaaatc
     6121 gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggtgaagg gcgtaacctg
     6181 aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg cgggctgaag
     6241 cggggaagag tgattatggc tgccagcgat caaatggtct ggcaggggga actggcagtt
     6301 gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag cccaccgatt
     6361 ttagttctga cgccgaaaaa tgccgaccga gaacatatcc gccgctcgct gtcaccaggg
     6421 ggatttcgtc cggtctattt ttatcagcac acatcagcgg ctaagaaata accttcacca
     6481 tgttgcgtca ccagtaaatc cgcgctgagt ttatgacgta aacgacgaat taacacatcg
     6541 acggtgcgca ggtcagggtt ttccacccga cgcgcagaaa gcatacgtag cagacgttca
     6601 cggctgagaa tttcgcccgg attcgtcaca aatgccacca acatttcata ctctgcgcgg
     6661 gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt caggcaataa
     6721 ccggcaaagc gatagcagtt gtcctgagtg tgcggttgag cttgtcgcgc gaggtcgatt
     6781 cgccagagca gatttttcac ccgtactacc agttcgcgca gttccagcgg tttggtgacg
     6841 taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct gcgtccggta
     6901 accagaataa tccccaccgt tgagcgttct cgcagggcgc gggttaacat caggccattt
     6961 tcatcgggta agttgatatc cagcagaatt aaatctaccg gctgattctg cataatttcc
     7021 cgcagcccgg caccgctcgc tgtaacggaa acggtatacc cctcctgagt gaagtaagat
     7081 tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg tggcatcaga
     7141 gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt catcctgacc
     7201 agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat tttacagggg
     7261 ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg tcggtactgg
     7321 cgctggtcgc aattgggatt gtgattggca ttgcgctgat tgtattgcca cacgttggga
     7381 tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa ccggtgtatg
     7441 aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct gaatgccatg
     7501 actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa gcgagcaatg
     7561 atatctacca gacctttatt gctcactcca ttgatacacc tgaaaaattc gaagccaaac
     7621 gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg gcaacctgcc
     7681 gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa gcggcacgtc
     7741 agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa ggtattgccc
     7801 accagttacc ggatatgagt agcggcttcc gtaagcagtt cgatgagctg cgcgccagtg
     7861 ctaatgacag tggtgacacg ctgtactcta ttgatattaa gccaatttat gcggcgaaag
     7921 gcgataaaga tgcctctggt tctctgctgc ctgcttcgga agtgaaagtc cttaaacgtg
     7981 acggcgactg gctgcaaatt gaaattaccg gctggacgga aagcgccgga cgtcagcgtg
     8041 tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt gatgtgcagc
     8101 agcaggtaaa aacgctggag aaaaccaccg ttgccgacac caataccgag tggagcaagt
     8161 tgcaggccac cgcatggatg aagaaaggcg acatggtgaa cgatatcaaa ccgatctggg
     8221 cctatgcgga ttcgttgtac aacggcacct gtaaccagtg ccacggcgca ccggaaatcg
     8281 cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc tttaccagtc
     8341 tcgataaacg tgaagaacgc actttgttga aatatctgca aatgaatgcg tctgacaccg
     8401 caggtaaggc tcacggcgat aagaaggaag aaaaataatg aacaataacg atctctttca
     8461 ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg ggatgctggg
     8521 gccgtcattg ttaacgccgc gccgtgcaac tgcggcgcaa gcggcgactg aggctgtcat
     8581 ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga cggtgaagga
     8641 tggtcgcttt gtggcggcaa aaccgttcga actggataaa tatccgtcga aaatgatcgc
     8701 cggattgccg gatcacgtac acaacgcggc acgtattcgt tatccgatgg tacgcgtgga
     8761 ctggctgcgt aagcgccatc tgagcgacac ctcccagcgc ggtgataacc gttttgtgcg
     8821 cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg tacagaaaac
     8881 tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga tgttccataa
     8941 cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgttg gtacgggcgg
     9001 agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct caatggaagt
     9061 gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca ttgtgctgtg
     9121 gggctccgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc acgatgttta
     9181 tgaatattac gagcagctaa aagcgaaagt cgccgccggt gaagttgagg tcatcagcat
     9241 cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga agcacattgc
     9301 ggttaacccg caaactgacg tgccgctgca actggcgctg gcacatacgc tgtacagtga
     9361 aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg ggttttgagc agttcctgcc
     9421 gtatctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg aaaaactgac
     9481 cggcatcgat gccgaaagca ttcgtgggct ggcgcggcag atggcggcga acagaacgca
     9541 gattattgct ggctggtgcg tacagcgtat gcagcacggt gaacagtggg cgtggatgat
     9601 tgtggttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggct ttggttttgg
     9661 ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc
     9721 cggctctacg tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac
     9781 tattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa
     9841 cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt
     9901 ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat
     9961 cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtac tgcctgcgac
    10021 cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat
    10081 cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg
    10141 cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg acgaaatggg
    10201 ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct
    10261 gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat
    10321 gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag
    10381 tggcctgatt gagatctact cgaaaaccat cgctgatatg aactacgacg attgtcaggg
    10441 gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta
    10501 tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt tatgtgagtc
    10561 ggaaactctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca
    10621 ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg
    10681 tcaggtgttg gcaggggcag tagtttctga ccgctatgca cccggcgtgg cacgaattca
    10741 cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg
    10801 taaccccaac gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg cgaccagtgc
    10861 gcacactacg ctggtggaaa ttgagaagta caatggagca gtggagcagg tgacggcgtt
    10921 taacggccct gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc
    10981 atggccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc
    11041 tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg
    11101 ttttcgttgc tgaaaagcga accgccgctg gctgcggcgg tgaatgagct ggaaaactgt
    11161 attgccacgc tgacagtacg tgacgatgcc cgtctggaac tggccgctga cttttgcggc
    11221 ctgtttttga tgactgacaa gcaggcggcg ctgccgtatg catcggccta caaacaggac
    11281 gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagcgg caatttcaac
    11341 gaaccggcag atcatctggc gatctatctc gagttgttga gtcatctgca tttttcgctg
    11401 ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac actgacggcg
    11461 ctgcggcaat ggttaccaga gtttgctgcg cgttgccatc agcatgacag ctttggtttt
    11521 tacgcggcac taagccagtt attgctggtg ttagtggagt gcgaccacca aaacagataa
    11581 cgtcgtttgt gcgcctgaaa agacgcgttt agcgtcgcat caggcattat ggggcagttg
    11641 ccggatgcgt catcacatcc ggcaaacaca catcacggat gagctacaaa ccgggaaagt
    11701 cgctggcgca gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat taacgtcagc
    11761 gccaccgcga tcccaggcca gtccagagcc agttcatgac gcaggcgtac tgcgcgttgc
    11821 accacgatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc acgcggttca
    11881 accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt atgcaggcaa
    11941 aattcggtaa tagtaaaagt caccgtaaca ttagccatta tgctttcccc caatctttac
    12001 gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca gtgttttcat
    12061 ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc tgttttttgc
    12121 tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcca ggcgggatag
    12181 tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc gccgcttccc
    12241 acgggctaac cggcaccaca atttccagat cctggccgac aatatcgaac agcggatgtg
    12301 gcgcaatatg aatcaccagc cacaaatcgc catttggacc gccattttcg cccggcgtcc
    12361 cctggccttt caggcggatg cgttgaccat tgccgacgcc cgccgggatc ttcacattca
    12421 gcgttttcgg gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc aggttatagc
    12481 tgatggtacg cttatgctca gtcagcgttt cttcgaggaa taccgccact tcgatttcaa
    12541 tatcgtggcc gcgtgtggcg gggcgttgac ggctctggcg ggcatgctga ccgaaaattg
    12601 acgagaagat atcgtcaaaa tcttcggcgt taaaactctg accgtcgcca tgctggaact
    12661 gacggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca gcgcgacgtt
    12721 gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct tcggcatccg
    12781 gttctttgct gacatcagga tggtatttgc gggcaagtcg acgataggcg gtcttgattg
    12841 tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt aattccatag
    12901 cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac tcctataagt
    12961 gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa atcatcgcgg
    13021 agaggaggtc gcctcaaggt ggggtgctga acatatttta aacaggtgaa aaagggtgag
    13081 cgatttttga tagtggaacc aggcacttta agtttaactg gggcgtcatt atttattaaa
    13141 ttttataggc g
//
LOCUS       DAZFAH010000128        13113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFAH010000128 DAZFAH010000000
VERSION     DAZFAH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.128"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(66..212)
                     /gene="ygdT"
                     /locus_tag="ACHJWB_003336"
     CDS             complement(66..212)
                     /gene="ygdT"
                     /locus_tag="ACHJWB_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4314911.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            398..1087
                     /gene="mutH"
                     /locus_tag="ACHJWB_003337"
     CDS             398..1087
                     /gene="mutH"
                     /locus_tag="ACHJWB_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4314912.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGACAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGELSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1156..1869
                     /locus_tag="ACHJWB_003338"
     CDS             1156..1869
                     /locus_tag="ACHJWB_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4314913.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2007..2225
                     /gene="ygdR"
                     /locus_tag="ACHJWB_003339"
     CDS             2007..2225
                     /gene="ygdR"
                     /locus_tag="ACHJWB_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4314914.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2333..3373
                     /locus_tag="ACHJWB_003340"
     CDS             2333..3373
                     /locus_tag="ACHJWB_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4314915.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="ACHJWB_003341"
     CDS             complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="ACHJWB_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4314916.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4591..6750)
                     /gene="aas"
                     /locus_tag="ACHJWB_003342"
     CDS             complement(4591..6750)
                     /gene="aas"
                     /locus_tag="ACHJWB_003342"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4314917.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARLAVRPRETLYESLLSAMYRFG
                     SGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7335..8366
                     /gene="galR"
                     /locus_tag="ACHJWB_003343"
     CDS             7335..8366
                     /gene="galR"
                     /locus_tag="ACHJWB_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4314918.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8373..9635)
                     /gene="lysA"
                     /locus_tag="ACHJWB_003344"
     CDS             complement(8373..9635)
                     /gene="lysA"
                     /locus_tag="ACHJWB_003344"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4314919.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9757..10692
                     /gene="lysR"
                     /locus_tag="ACHJWB_003345"
     CDS             9757..10692
                     /gene="lysR"
                     /locus_tag="ACHJWB_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4314920.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10679..11371)
                     /gene="ygeA"
                     /locus_tag="ACHJWB_003346"
     CDS             complement(10679..11371)
                     /gene="ygeA"
                     /locus_tag="ACHJWB_003346"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4314921.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11500..12918)
                     /gene="araE"
                     /locus_tag="ACHJWB_003347"
     CDS             complement(11500..12918)
                     /gene="araE"
                     /locus_tag="ACHJWB_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4314922.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3340 a         3512 c         3208 g         3053 t
ORIGIN      
        1 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
       61 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
      121 ctaaaaactg gagtttcatc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
      181 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
      241 ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc ctaaaaagca
      301 atataaatca gggaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac
      361 ataattgctg tttgtttttt aatcaaggta tcatgacatg tcccaacctc gcccactgct
      421 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
      481 attgggagaa ctggcggcac ttgccgggct tgttacgcca gagaatttaa aacgcgataa
      541 gggctggatt ggcgtgttac tggagatctg gctaggtgcc tgcgcaggga gtaaacctga
      601 gcaagatttt gccgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
      661 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
      721 ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
      781 gctcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga
      841 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
      901 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
      961 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
     1021 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
     1081 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
     1141 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
     1201 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
     1261 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
     1321 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
     1381 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
     1441 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
     1501 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
     1561 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
     1621 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
     1681 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg
     1741 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
     1801 ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat
     1861 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt ttaccctcac
     1921 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
     1981 taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
     2041 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
     2101 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
     2161 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
     2221 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
     2281 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
     2341 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
     2401 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
     2461 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
     2521 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
     2581 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
     2641 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
     2701 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
     2761 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
     2821 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
     2881 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
     2941 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
     3001 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg
     3061 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
     3121 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
     3181 tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc
     3241 cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca
     3301 tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatactta
     3361 tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
     3421 cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc
     3481 acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
     3541 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
     3601 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac gccaatcagc
     3661 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
     3721 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct
     3781 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtgggcgta
     3841 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
     3901 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc
     3961 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
     4021 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
     4081 gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac accaccggct
     4141 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agctttcact
     4201 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
     4261 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
     4321 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
     4381 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
     4441 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
     4501 agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
     4561 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
     4621 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
     4681 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
     4741 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
     4801 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
     4861 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
     4921 ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt cataccagcc
     4981 gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
     5041 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
     5101 gataccaggg accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
     5161 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg tcacgccgta
     5221 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtaa
     5281 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
     5341 cgcgtagtga ccgaggaaag tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac
     5401 caactccggc acaatacggt aatgcagcgg gcttggataa agaaacactt ctgcacctgt
     5461 gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga
     5521 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cgttcgccag
     5581 aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaagag
     5641 gatcagcgcc tcttcttccg gctgctgttt tacctgtgcc agacgtggca tcagcagatg
     5701 cgcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
     5761 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
     5821 gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca gccctttcac
     5881 cccggcagtg tagttcatca ttgcgggaat gcggcgacgg gctatggccc caaaaatcac
     5941 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
     6001 tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtctgg
     6061 ggtaaagttg acgtcttcga cacatttctt cccggatccg aagcggtaca ttgcactcag
     6121 taaagattcg tacagcgttt cacgcgggcg caccgccagt cgcgcttcca tcattatttg
     6181 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc
     6241 cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
     6301 acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt
     6361 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
     6421 agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
     6481 cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
     6541 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca ctggacgcac
     6601 aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag gcgtaattag
     6661 aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
     6721 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
     6781 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
     6841 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
     6901 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
     6961 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
     7021 ttacagattg atgaccggca aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg
     7081 tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact ttagcaacct
     7141 gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta accaaaggtt
     7201 tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac gatgaaaaca
     7261 cgccacccct tgaaccaacg ggcgttttcc gtaacactga aagaatgtaa gcgtttaccc
     7321 actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc gtttcagtcg
     7381 ccaccgtttc ccgcgtcatt aataattcac ctaaagccag tgaagcttcc cggctggctg
     7441 tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt gcgctggcgc
     7501 agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg
     7561 caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta ttgattggca
     7621 acggttacca caacgaacaa aaagagcgtc aggctattga gcaactgatc cgccatcgct
     7681 gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc tcattaatga
     7741 aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta
     7801 ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc
     7861 atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa gatcgtctgc
     7921 aagggtatta cgatgccctt gctgaaagtg gtattgcggc caatgaccgg ctggtgacat
     7981 ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa
     8041 gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggtgcg atgggcgttc
     8101 tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt gatgatgtgc
     8161 tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc gtgacgatgg
     8221 cgacccaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc ccggaaatca
     8281 ctaatgtctt tagtccgacg ctggtacgtc gtcattcagt gtcaactccg tcgctggagg
     8341 caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc cagtaattct
     8401 tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag aacttctggt
     8461 aacagcggac ggctattgta gttggatgac attgatgcgc catatgcccc tgtatcatgc
     8521 agtaccagat aatcacccgc cttcacttcc ggcaaggcgc gggtttcaac atttccccct
     8581 tcctgctggg taaagacatc gcccgattca cataatggcc cggcgacgac ggtttccacc
     8641 gttggcgcgt gttccagaga acgaccatca gctgccaggg cactgatatg gtggtaacta
     8701 ccgtacattg ccgggcgcat cagatcgttg aacccggcat caaccagcac aaagtggcgg
     8761 ctacccattt gtttgacgct ccgcacctga gtaattaaca cgccagactg cgctaccaga
     8821 aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgagc gatttgctca
     8881 cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc accctgttga
     8941 taaggaatag aaagcccacc gcccgcagaa atagcctgta aatcctgacc gaattcgatg
     9001 acctgacgta ccatagcacc acacacctgt tccagatggg cataatcaac gccagaacca
     9061 atgtgcatgt gaatgccgac cagctgcaaa tgatgacgtt gtatcacgtc cagtgcggcg
     9121 ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt ggttttttgg
     9181 ctatgtccgt gaccaaaccc cgggttaacg cgcagccata cccgatgccc tggcgaaacc
     9241 tggcccagtt ggtcgagcat atcaacagaa cccgcattca ccggaatttg caattcactg
     9301 acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc atcggggtgc
     9361 gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga gacggaatcc
     9421 actttcacgc cctgctcacg cattaatcgc aaaatatgaa tattggaaca ggctttctgt
     9481 gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat aatttgcgca
     9541 tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt ttcggcggtg
     9601 agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagatg agtgcttttt
     9661 tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga gtctatgcaa
     9721 aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat
     9781 attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc acacctgcta
     9841 cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta
     9901 aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact gcgtctgttt
     9961 gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc agaaagtctg
    10021 cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc gcaatctttt
    10081 ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt aaatatcgtg
    10141 ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga tttaggactc
    10201 actgagacac tccatacgcc tgcgggaaca gaacgtaccg aattactctc tttagatgaa
    10261 gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac gccggatgat
    10321 tttcagggtg agaactacat cagcctttcc cgtactgaca gctatcgcca gttgctggat
    10381 cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg
    10441 tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca
    10501 ctggattatg cggcaagcgg tttagtggta cggcggttca gtattgcggt tccgttcacc
    10561 gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag
    10621 catttacaag cggggttacc gaaactggtc acttctcttg acgcgatttt gtcgtcagct
    10681 acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcagtatca aacacaggca
    10741 aaacactgcg ctcttctgga accaataaac caatttctgt gcagccaaaa atgacaccct
    10801 gcgccccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg
    10861 taaattgccc cagacacagt tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg
    10921 cttccggaat aagacaattg atggaaaatt gttccgtcag ccgcccgcga taaaaatcct
    10981 gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc ccggtaattg
    11041 cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat
    11101 ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc gcccgctgta
    11161 agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg cgacgctggc
    11221 actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag tgaagcccac
    11281 caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg gattcccagc
    11341 tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg ttaccgcaac
    11401 aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccaatc tctattaacg
    11461 aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga tatttctcaa
    11521 cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt tggtttccgg
    11581 aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt
    11641 accggcagcg ccaatgctgt cgagcagcgt caggaaggtc gcgccgataa tcatattcga
    11701 cacccagttc gtggtggtcg aacaggtgat gccaaaatcg cggcatttca gcggctgaat
    11761 ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg caatacacat
    11821 catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt tatcaaactg
    11881 catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac caattttcag
    11941 agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg cgaacataaa
    12001 ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat
    12061 tttgaagata cgcggcgcat agtacatgat gatgttcata ccggtaaact gctgcatcgc
    12121 ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct taaacagtgc
    12181 ccaaccgcct tgttttaact tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt
    12241 ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac gccccttttc
    12301 cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca ggactgctgg
    12361 taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga
    12421 taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc
    12481 gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc
    12541 gacagcaatg cccagcacca cacgagcggc gattaacatc tctacgctgg tcgcaaaagc
    12601 ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc
    12661 caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcaccga gcatcatgct
    12721 actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa
    12781 cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac
    12841 cgaaacaaac atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta aagcagattc
    12901 cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg aaaaaatacg
    12961 tgaacaactc acacaggtgt caggtcggaa acagcataaa tatggattaa attgctacga
    13021 catgtcgtta tgtgatggat attccaattt tcaaattaag ttgaattatt gatattatta
    13081 ttaaccacct aattttacag cagataaaat tca
//
LOCUS       DAZFAH010000129        13057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFAH010000129 DAZFAH010000000
VERSION     DAZFAH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.129"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHJWB_003348"
     CDS             complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHJWB_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4314923.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(923..2353)
                     /locus_tag="ACHJWB_003349"
     CDS             complement(923..2353)
                     /locus_tag="ACHJWB_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4314924.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2632..3585
                     /gene="tal"
                     /locus_tag="ACHJWB_003350"
     CDS             2632..3585
                     /gene="tal"
                     /locus_tag="ACHJWB_003350"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4314925.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3700..4287
                     /gene="mog"
                     /locus_tag="ACHJWB_003351"
     CDS             3700..4287
                     /gene="mog"
                     /locus_tag="ACHJWB_003351"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4314926.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHJWB_003352"
     CDS             complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHJWB_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4314927.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHJWB_003353"
     CDS             complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHJWB_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4314928.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLI
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5776..6180)
                     /locus_tag="ACHJWB_003354"
     CDS             complement(5776..6180)
                     /locus_tag="ACHJWB_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4314929.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHJWB_003355"
     CDS             6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHJWB_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4314930.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHJWB_003356"
     CDS             8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHJWB_003356"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4314931.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHJWB_003357"
     CDS             complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHJWB_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4314932.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            10534..11700
                     /gene="nhaA"
                     /locus_tag="ACHJWB_003358"
     CDS             10534..11700
                     /gene="nhaA"
                     /locus_tag="ACHJWB_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4314933.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11766..12665
                     /gene="nhaR"
                     /locus_tag="ACHJWB_003359"
     CDS             11766..12665
                     /gene="nhaR"
                     /locus_tag="ACHJWB_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4314934.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(12704..>13057)
                     /locus_tag="ACHJWB_003360"
     CDS             complement(12704..>13057)
                     /locus_tag="ACHJWB_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4314935.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT         3356 a         3185 c         3339 g         3177 t
ORIGIN      
        1 tctgcaatat attgaatctg catgcttttg taggccggat aaggcgttca cgccgcatcc
       61 ggcattgact acaaacttaa cgctgctcgt agcgtttaaa caccagttcg ccattgctgg
      121 aggaatcttc atcaaagaag taaccttcgc tattaaaacc agtcagttgc tctggtttgg
      181 tcagccgatt ttcaataata aaacgactca tcagaccgcg tgctttctta gcgtagaagc
      241 tgattatctt aaatttgccg ttcttctcat cgaggaacac cggcttgata atctcggcat
      301 tcaatttctt cggcttcacc gatttaaaat actcatctga cgccagatta atcaccacat
      361 tatcgccttg tgctgcgagc gcctcgttca gcttgttggt gatgatatct ccccagaatt
      421 gatacagatc tttccctcgg gcattctcaa gacggatccc catttccaga cgataaggct
      481 gcattaaatc gagcgggcgc agtacgccat acaagccgga aagcattcgc aaatgctgtt
      541 gggcaaaatc gaaatcgtct tcgctgaagg tttcggcctg caagccggtg tagacatcac
      601 ctttaaacgc cagaatcgcc tggcgggcat tctccggcgt gaaatctggc tgccagtcat
      661 gaaagcgagc ggcgttgata cccgccagtt tgtcgctgat gcgcatcagc gtgctaatct
      721 gcggaggcgt cagtttccgc gcttcatgga tcaactgctg ggaattgtct aacagctccg
      781 gcagcgtata gcgcgtggtg gtcaacgggc tttggtaatc aagcgttttc gcaggtgaaa
      841 taagaatcag catatccagt ccttgcagga aatttatgcc gactttagca aaaaaagaga
      901 atgagttgat cgatagttgt gattactcct gcgaaacatc atcccacgcg tccggagaaa
      961 gctggcgacc gatatccgga taacgcaacg gatcaaacac cgggcgcacg ccgagtttac
     1021 gctggcgtag ataatcactg gcaatggtat gaaccacagg cgagagcagt aaaatggcgg
     1081 tcaaattggt aatagccatg caggccatta tgatatctgc cagttgccac atcagcggaa
     1141 ggcttagcaa ggtgccgccg ataaccgttg cgaaggtgca gatccgcaaa caccagatcg
     1201 ctttagggtt gttcaggcgt aaaaagaaga gattgttttc ggcataaatg tagttggcaa
     1261 cgatggagct gaaggcaaac agaataacca cgagggtaac aaactcagca ccccaggaac
     1321 ccattagcac ccgcatcgcc ttctggataa gctgaatacc ttccagcggc atgtaggttg
     1381 tgtcgttacc cgccagtaat atcagcatgg cgcttgccgt acagatgacc agggtgtcga
     1441 taaaaatgcc aatcatctgg acaatccctt gcgctgccgg atgcggaggc caggacgccg
     1501 ctgccgctgc cgcgtttggc gtcgaaccca ttcccgcctc attggaaaac atactgcgct
     1561 gaaaaccgtt agtaatcgcc tggcttaagg tatatcccgc cgcgccgcct gccgcttcct
     1621 gccagccaaa agcactctca aaaatagacc aaatgacgtg gggaagttgc ccgatattta
     1681 ttacgcaaat caccatgctg gtcagcaccc agattatcgc catcaacggg acaaacccct
     1741 gcatgagccg ggcgacgcca tgaagaccgc gagtgattgc cagcagagca aagacagcga
     1801 gaataatgcc tgtcaccagc gggggaaaat caaaagaaaa actcagggct cgggcaacgg
     1861 cgttcgcctg aactccgctg aaaattatgc cgtaggcgat gagcaaaaag acggcgaaca
     1921 gaacgcccat ccaacgcatc cccagcccgc gcgccatata ccatgccggt cctccacgaa
     1981 actgtccatt gacgtcacgt tctttataaa gttgtgcaag ggaacattcg gcaaacgagg
     2041 tcgccatgcc gataaacgcg gcaacccaca tccaaaagac ggctccaggt ccaccggcgg
     2101 taatagccag cgcaacgccg gccaggttgc cgctacccac gcgcgccgca agactggtac
     2161 acaatgactg aaatgaggtt aaaccgcctg gctgtggatg aatgctattt ttaagacttt
     2221 tgccaaactg gcggatgtag cgaaactgca caaatccggt gcgaaaagtg aaccaacaac
     2281 ctgcgccgaa gagcaggtaa atcattaccg atccccaaag gacgctgtta ataaaggaga
     2341 aaaaatctgg catgcatatc cctcttattg ccggtcgcga tgactttcct gtgtaaacgt
     2401 taccaattgt ttaagaagta tatacgctac gaggtacttg ataacttctg cgtagcatac
     2461 atgaggtttt gtataaaaat ggcgggagat atcaacgcag tgtcagaaat ccgaaacagt
     2521 ctcgcctggc gataaccgtc ttgtcggcgg ttgcgctgac gttgcgtcgt gatatcatca
     2581 gggcagaccg gttacatccc cctaacaagc tgtttaaaga gaaatactat catgacggac
     2641 aaattgacct cccttcgtca gtacaccacc gtagtggccg acactgggga catcgcggca
     2701 atgaagctgt atcaaccgca ggatgccaca accaaccctt ctctcattct taacgcagcg
     2761 cagattccgg aataccgtaa gttgattgat gatgctgtcg cctgggcgaa acagcagagc
     2821 aacgatcgcg cgcagcagat cgtggacgcg accgacaaac tggcagtaaa tattggtctg
     2881 gaaatcctga aactggttcc gggccgtatc tcaactgaag ttgatgcgcg tctttcctat
     2941 gacaccgaag cgtcaattgc gaaagcaaaa cgcctgatca aactctacaa cgatgctggt
     3001 attagcaacg atcgtattct gatcaaactg gcttctacct ggcagggtat ccgtgctgca
     3061 gaacagctgg aaaaagaagg catcaactgt aacctgaccc tgctgttctc cttcgctcag
     3121 gctcgtgctt gtgcggaagc gggcgtgttc ctgatctcgc cgtttgttgg ccgtattctt
     3181 gactggtaca aagcgaatac cgataagaaa gagtacgctc cggcagaaga tccgggcgtg
     3241 gtttctgtat ctgaaatcta ccagtactac aaagagcacg gttatgaaac cgtggttatg
     3301 ggcgcaagct tccgtaacat cggcgaaatt ctggaactgg caggctgcga ccgtcttacc
     3361 atcgcaccta cactgctgaa agagctggct gagagcgaag gggctatcga acgtaaactg
     3421 tcttacaccg gcgaagtgaa agcgcgtccg gcgcgtatca ctgagtccga gttcctgtgg
     3481 cagcacaacc aggatccaat ggcagtagat aaactggcgg aaggtatccg taagtttgct
     3541 attgaccagg aaaaactgga aaaaatgatc ggcgatctgc tgtaatcatt cttagcgtga
     3601 ccgggaagtc ggtcacgcta cctcttctga agcctgtctg tcactccctt cgcagtgtat
     3661 cattctgttt aacgagactg tttaaacgga aaaatcttga tgaatacttt acgtattggc
     3721 ttagtttcca tctctgatcg cgcatccagc ggcgtttatc aggataaagg catccctgcg
     3781 ctggaagaat ggctgacatc ggcgctaacc acgccgtttg aactggaaac ccgcttaatc
     3841 cccgatgagc aggcgatcat cgagcaaacg ttgtgtgagc tagtggatga aatgagttgc
     3901 catctggtgc tcaccacggg cggaactggc ccggcgcgtc gtgacgtaac gcccgatgcg
     3961 acgctggcag tagcggaccg cgagatgcct ggctttggtg aacagatgcg ccagataagc
     4021 ctgcattttg taccaactgc gatcctttcg cgtcaggttg gtgtgattcg caaacaggcg
     4081 ctgatcctta acttacccgg tcagccgaag tctattaaag agacgctgga aggtgtgaag
     4141 gacgctgcgg gtaacgttgt ggtacacggt atttttgcca gcgtaccgta ctgcattcag
     4201 ttgctggaag ggccatacgt tgaaacggca ccagaagtgg ttgctgcatt cagaccgaag
     4261 agtgcaagac gcgacgttag cgaataaaaa aatccccccg agcgggggga tctcaaaaca
     4321 attagtggga ttcaccaatc ggcagaacgg tgcgaccaaa ctgctcgttc agtacttcac
     4381 ccatcgccag atagattgca ctggcaccac agatcagacc aatccagccg gcaaagtgga
     4441 tgattgcggc gttaccggca atgttaccga tcgccagcag ggcaaacagc acagtcaggc
     4501 taaagaaaac gaattgcaga acgcgtgcgc ctttcagcgt gccgaagaac ataaacagcg
     4561 taaatacgcc ccacagaccc aggtagacac caaggaactg tgcatttggc gcatcggtca
     4621 gacccagttt cggcatcagc agaatcgcaa ccagcgtcag ccagaaagaa ccgtaagagg
     4681 tgaatgcggt taaaccgaaa gtgttgcctt ttttgtactc cagcagaccg gcaaaaattt
     4741 gcgcgatgcc gccgtagaaa atgcccatgg caagaataat accgtccaga gcgaaataac
     4801 ccacgttgtg caggttaagc agaatggtgg tcatgccgaa gcccatcagg cccagcggtg
     4861 ccggattagc caacttagtg ttgcccataa ttcctcaaaa atcatcatcg aatgaatggt
     4921 gaaataattt ccctgaataa cagtagtgtt ttcagggcgc ggcataataa tcagccagtg
     4981 gggcagtgtc tacgatcttt tgaggggaaa atgaaaattt tccccggttt acggtgtcag
     5041 acctgagtgg cgctaaccat ccggcgcagg caggcgattt gcagtacggc tggaattgtc
     5101 acgcgatagg cgctgccgct gaccgcttta accccattta atgctgcgcc cacagggcct
     5161 cccagacccg cgccgcgcag caaaccatgc ccaagcacgc tcattgctgc gtgggtgcgt
     5221 aaaatgcggg tcaattggct ggaaagcaaa tgcgacacgc cttttgccaa taatttgtct
     5281 ttcatcagca gcggcagcag ctcttccagc tcattcaccc tggcatcgac cgcgtgcaga
     5341 aactcctgct tatgttcctc gtccatattc ttccaggtgt tacgcagaaa ttgttccagt
     5401 aactgttgct caatctcaaa cgtagacatc tctttgtcgg ctttcagctt caatcgtttt
     5461 gaaacatcga gcaaaatggc ccgatacaat ttaccgtgtc cgcgcagttt gttggcgata
     5521 ctatcgccac caaaatgctg taattctcca gcaatcagct gccagttgcg gcgatgttgc
     5581 tcgggatgcc cttccatcga tttaaacagt tcgttgcgta tcagtacgct ggagaggcga
     5641 gttttgcctt tttcattatg ggtgagcaat cgggcgaaat ttgccaactg ttcctcacta
     5701 caatgctgaa gaaaatccag atctgaatca ttcaggtaat taacattcat tttttgtggt
     5761 ttctatattc tggcgttagt cgtcgccgat aattttcagc gtggccatat ccgatgagtt
     5821 caccgtatga ccggaaaagg tgatttttga gacgcagcgt ttattgtcgt tatcactgtt
     5881 aatgttgatc cagtcagtgg tttgcccttc ttttatttct gaaggaatat tcagactctg
     5941 actggcgcta cgggcggctt tgaaataaac cgatgcaccg cttaactgta aatcgccatg
     6001 gtcggcagag agttgtatgc gtttcacaat gcgacaaaca ggaagtttca gcgccagatc
     6061 gttggtttcg ttacgcggca ttgcaatgac gccgaggagt ttatggtcgt ttgcctgcgc
     6121 cgtgcagcac agcatcaggc taatcgccag gctggcggaa atcgtaaaaa cggatttcat
     6181 aaggattctc ttagtgggaa gaggtagagg gatgaatacc cactagttta ctgctgataa
     6241 agagaaggtt caggtacgta atcttttctt tttattacaa ttttttgatg aatgccttgg
     6301 ctgcgattca ttctttatct gaataaaatt gctgtcaatt ttacgtcttg tcctgccata
     6361 tcgcgaaatt tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg
     6421 ttacgacccc atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc
     6481 agttgaaacc agacgtttcg cccctattac agactcacaa ccacatgatg accgaatata
     6541 tagtggagac gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg
     6601 tgtagcgatt atggatggca ccactcctcg tgtgctggag aacgccgaag gcgatcgcac
     6661 cacgccttct atcattgcct atacccagga tggtgaaact ctggttggtc agccggctaa
     6721 acgtcaggca gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg
     6781 ccgcttccag gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc
     6841 tgctgataac ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat
     6901 ctctgctgaa gtgctgaaaa aaatgaagaa aaccgctgaa gattacctgg gtgaaccggt
     6961 aactgaagct gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa
     7021 agacgcaggc cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc
     7081 tgcgctggct tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct
     7141 gggtggtggt actttcgata tttctattat cgaaatcgac gaagttgacg gcgaaaaaac
     7201 cttcgaagtt ctggcaacca acggtgatac ccacctgggt ggtgaagact tcgacagccg
     7261 tctgatcaac tacctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga
     7321 tccgctggca atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc
     7381 cgctcagcag accgacgtta acctgccgta catcactgca gatgcgaccg gtccgaaaca
     7441 catgaacatc aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc tggtaaaccg
     7501 ttccattgag ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga
     7561 cgacgttatc ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga
     7621 gttctttggt aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc
     7681 tgctgttcag ggtggtgttc tgactggtga cgtgaaagac gtactgctgc tggacgttac
     7741 cccgctgtct ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa
     7801 caccactatc ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc
     7861 ggtaaccatc catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg
     7921 tcagttcaac ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac
     7981 cttcgatatc gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa
     8041 agagcagaag atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat
     8101 ggtacgcgac gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac
     8161 tcgcaaccag ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga
     8221 caaactgccg gctgacgaca aaactgctat cgagtctgca ctgactgcac tggaaactgc
     8281 tctgaaaggt gaagacaaag ccgctatcga agcgaaaatg caggaactgg cacaggtttc
     8341 ccagaaactg atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga
     8401 tgcttctgca aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa
     8461 agacaaaaaa taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt
     8521 cctctccgcc cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt
     8581 acgagatttt aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac
     8641 gcctggccat gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat
     8701 ttaaagagat caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg
     8761 atcagtatgg tcatgctgcg tttgagcaag gtggtatggg cggcggcggt tttggcggcg
     8821 gcgcagactt cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg
     8881 gtcgtcaacg tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag
     8941 aagctgtacg tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt
     9001 gccacggtag cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt
     9061 ctggtcaggt gcagatgcgt cagggtttct ttgccgtgca gcagacctgt ccacactgtc
     9121 agggtcgcgg tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg
     9181 agcgcagcaa aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc
     9241 gtcttgcggg cgaaggtgaa gcgggcgagc atggcgcacc ggcaggcgat ctgtacgttc
     9301 aggttcaggt taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag
     9361 tcccgatcaa cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg
     9421 gtcgcgtcaa actgaaagtg cctggcgaaa cccagaccgg taagcttttc cgtatgcgcg
     9481 gtaaaggcgt caagtctgtc cgcggtggcg cacagggtga tttgctgtgc cgcgttgtcg
     9541 tcgaaacacc ggtaggcctg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa
     9601 gcttcggtgg cccaaccggc gagcacaaca gcccgcgctc aaagagcttc tttgatggtg
     9661 tgaagaagtt ttttgacgac ctgacccgct aacctcccca aaagcctgcc cgtgggcagg
     9721 cttgggtaaa aatagggtgc gttgaagata tgcgagcacc tgtaaagtgg cggggatcac
     9781 tccccgccgt tgctcttact cggattcgta agccgtgaaa acagcaacct ccgtctggcc
     9841 agttcggatg tgaacctcac agaggtcttt tctcgttacc agcgccgcca ctacggcggt
     9901 gatacagatg acgatcaggg cgacaatcat cgccttatgc tgcttcattg ctctcttctc
     9961 cttgacctta cggtcagtaa gaggcactct acatgtgttc agcatatagg gggcctcggg
    10021 ttgatggtaa aatatcactc ggggcttttc tctatctgcc gttcagctaa tgcctgagac
    10081 agacagcctc aagcacccgc cgctattata tcgctctctt ttacccattc tgttttatcg
    10141 attctaatcc tgaagacgcc tcgcattttt gtggcgtaat ttttttatga tttaattatt
    10201 taactttaat ttatctcttt atcgcaatta ttgacgacaa gctggattat ttttgaaata
    10261 ttgtcctaac aaataacgcc gactgacaag aaattaatta ttacttttcc taattaatcc
    10321 ctcagaaatc ctcaccttaa gctatgatta tctgggctta gggtcactcg tgagcgctta
    10381 cagccgtcaa aaactcatct caccgctgat ggcgcaaatt cttcaatggg tcgtaaaaaa
    10441 cgagttattc ctacactata atctgatttt aacgatgatt tgtgcggggt aaaatagtaa
    10501 aaacgatcta ttcacctgaa agagaaataa aaagtgaaac atctgcatcg attctttagc
    10561 agtgatgcct cgggaggcat tattcttatc attgccgcta tcctggcgat gattatggcc
    10621 aatagcggcg caaccagtgg atggtatcac gactttctgg agacgccggt tcagctccgg
    10681 gttggttcac tcgaaatcaa caaaaacatg ctgttatgga taaatgacgc gctgatggcg
    10741 gtatttttcc tgttagtcgg tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc
    10801 agcttacgcc aggccgcatt tccagttatc gccgctattg gtgggatgat tgttccggca
    10861 ttactctatc tggcctttaa ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg
    10921 gcggctactg acattgcctt tgcacttggt gtgctggcgc tgttgggaag tcgtgttccg
    10981 ttagcgctga agatcttttt gatggctctg gctattatcg acgatcttgg ggccatcatt
    11041 atcatcgcat tgttctacac taatgactta tcgatggcct ctcttggcgt cgcggctgta
    11101 gcaattgcgg tactcgcggt attgaatctg tgtggtgtac gccgcacggg cgtctatatt
    11161 ctggttggcg tagtgttgtg gactgcggtg ttgaaatcgg gggttcacgc aaccctggcg
    11221 ggggtaattg tcggcttctt tattcctttg aaagagaagc atgggcgttc tccagcgaag
    11281 cgactggagc atgtgttaca cccgtgggtg gcgtatctga ttttgccgct gtttgcattt
    11341 gctaatgctg gcgtttcact gcaaggtgtc acgctggatg gcttgacctc cattctgcca
    11401 ttggggatca tagctggctt gctgattggc aaaccgctgg ggattagtct gttctgctgg
    11461 ttggcgctgc gtttgaaact ggcgcatctg cctgagggaa cgacttatca gcaaattatg
    11521 gcggtgggga tcctgtgcgg tatcggtttt actatgtcta tctttattgc cagcttggcc
    11581 tttggtagcg tagatccaga actgattaac tgggcgaaac tcggtatcct ggtcggttct
    11641 atctcttcgg cggtaattgg atacagctgg ttacgcgttc gtttgcgtcc atcagtttga
    11701 caggacggtt taccggggag ccataaacgg ctcccttttc attgttatca gggagagaaa
    11761 tgagcatgtc tcatatcaat tacaaccact tgtattactt ctggcatgtc tataaagaag
    11821 gttccgtggt tggcgcagcg gaggcgcttt atttaactcc acaaaccatt accggacaga
    11881 ttcgagcgct ggaagagcgc ctgcaaggca aattatttaa acgcaaggga cgtggtctcg
    11941 aacccagcga gctgggagaa ctggtctatc gctatgccga taaaatgttc accttaagcc
    12001 aggaaatgct ggatattgtg aactatcgca aagaatccaa tttattgttt gacgttggtg
    12061 tggctgatgc actttccaaa cgcctggtca gtagcgtact taacgccgca gtggtagaag
    12121 gcgagcccat tcatcttcgc tgcttcgaat ccacccacga aatgctgctg gagcaattaa
    12181 gtcagcataa actggatatg atcatttctg actgtccgat agactctacg cagcaggaag
    12241 gcctgttctc cgtgagaatt ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac
    12301 cagaaaaacc gttcccggct tgtctggaag aacggcgact tttgattcct gggcgacgtt
    12361 caatgttagg gcgcaaattg cttaactggt ttaactccca gggattaaac gtagaaatcc
    12421 tcggcgagtt tgatgatgcc gcgttgatga aagcttttgg tgcgatgcac aatgcaatct
    12481 tcgttgcccc aacgctttat gcatatgact tttatgccga taaaactgtc gtagaaattg
    12541 gtcgcgtcga gaatgtgatg gaagagtacc atgctatttt tgctgagcgg atgattcagc
    12601 acccggcggt acagcgaatc tgcaatacgg attattctgc gctttttagt ccagcggcgc
    12661 gttaatcggc acctcccccc aaagttaagg tgggggagat agattagttg tacataacca
    12721 cgattttgac tcggctcatt atttgcccgc ttgagacatt atttccatat gtacgcgggc
    12781 gaataaatag aggaatctga ttacttcctt catggggatg ctgaaaagag tagtaattgc
    12841 ttttaccata ctggatttca ttcgtaccat cattggagag ttgaaagcct atttgacccg
    12901 caccgtcctc actgttttct aactcatttt ttaatatgct ggttcctgac tcctgtatcg
    12961 tcactggttc aaagtaatat tcaacatcac tgggttcaaa aactaattca ccttgctcgc
    13021 cctggatgca gtcaaatctt atatgatgaa actgata
//
LOCUS       DAZFAH010000130        13043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFAH010000130 DAZFAH010000000
VERSION     DAZFAH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.130"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            137..1246
                     /gene="livK"
                     /locus_tag="ACHJWB_003361"
     CDS             137..1246
                     /gene="livK"
                     /locus_tag="ACHJWB_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4314936.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1294..2220
                     /gene="livH"
                     /locus_tag="ACHJWB_003362"
     CDS             1294..2220
                     /gene="livH"
                     /locus_tag="ACHJWB_003362"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4314937.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2217..3494
                     /gene="livM"
                     /locus_tag="ACHJWB_003363"
     CDS             2217..3494
                     /gene="livM"
                     /locus_tag="ACHJWB_003363"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4314938.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3491..4258
                     /gene="livG"
                     /locus_tag="ACHJWB_003364"
     CDS             3491..4258
                     /gene="livG"
                     /locus_tag="ACHJWB_003364"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4314939.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4260..4973
                     /gene="livF"
                     /locus_tag="ACHJWB_003365"
     CDS             4260..4973
                     /gene="livF"
                     /locus_tag="ACHJWB_003365"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4314940.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGRTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5370..6686
                     /gene="ugpB"
                     /locus_tag="ACHJWB_003366"
     CDS             5370..6686
                     /gene="ugpB"
                     /locus_tag="ACHJWB_003366"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4314941.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6784..7671
                     /gene="ugpA"
                     /locus_tag="ACHJWB_003367"
     CDS             6784..7671
                     /gene="ugpA"
                     /locus_tag="ACHJWB_003367"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4314942.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDATTSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFLYVESKVRYQ"
     gene            7668..8513
                     /gene="ugpE"
                     /locus_tag="ACHJWB_003368"
     CDS             7668..8513
                     /gene="ugpE"
                     /locus_tag="ACHJWB_003368"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4314943.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8515..9585
                     /gene="ugpC"
                     /locus_tag="ACHJWB_003369"
     CDS             8515..9585
                     /gene="ugpC"
                     /locus_tag="ACHJWB_003369"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4314944.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     ICGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9582..10325
                     /gene="ugpQ"
                     /locus_tag="ACHJWB_003370"
     CDS             9582..10325
                     /gene="ugpQ"
                     /locus_tag="ACHJWB_003370"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4314945.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDLIGPNFTAQ"
     gene            complement(10312..10752)
                     /locus_tag="ACHJWB_003371"
     CDS             complement(10312..10752)
                     /locus_tag="ACHJWB_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4314946.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10872..12614
                     /gene="ggt"
                     /locus_tag="ACHJWB_003372"
     CDS             10872..12614
                     /gene="ggt"
                     /locus_tag="ACHJWB_003372"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4314947.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12652..12936)
                     /locus_tag="ACHJWB_003373"
     CDS             complement(12652..12936)
                     /locus_tag="ACHJWB_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO4314948.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2896 a         3335 c         3729 g         3083 t
ORIGIN      
        1 atcactatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa aacagtgcag
       61 tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac aacatcacga
      121 atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact
      181 ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg gcgcgatgtc
      241 cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga
      301 cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat atgacgacgc
      361 ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg gcattaaata
      421 cgttattggt catctgtgtt cttcttctac ccagcctgcg tcagatatct atgaagacga
      481 aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac gcggttatca
      541 acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg caaaatacat
      601 tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac aatatggcga
      661 agggctggca cgttcggtgc aggacgggct gaaagctgct aacgccaatg ttgttttctt
      721 cgacggcatt accgctggcg agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga
      781 aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca
      841 ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtgg gtaatgcgtc
      901 gctgtcgaat attgctggcg atgctgccga aggcatgttg gtcaccatgc caaaacgcta
      961 tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca agaaagatcc
     1021 gtccgggcct tatgtctgga tcacttacgc ggcggtgcaa tctctggcaa cagcccttga
     1081 gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcaa acggtgcaaa
     1141 caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg
     1201 tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat cccaccgccc
     1261 gtgaaaaacg ggcgggttta gaaaggttac cttatgtctg agcagttttt gtatttcttg
     1321 cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc catcggctac
     1381 accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt ttatatgatt
     1441 ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat tgataccggc
     1501 tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg
     1561 agtatcgaac gggtggctta ccgcccggtg cgtaattcta agcgcctgat tgcgcttatt
     1621 tccgcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga aggttcgcgc
     1681 gatgtggcgc tgcccagcct gtttaacggt cagtgggtgg tgggccatag cgaaaacttc
     1741 tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct cgccatgctg
     1801 gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgtcgtgc ctgcgcggaa
     1861 gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc tttgaccttt
     1921 gttattggcg cggcgatggc ggcagtggct ggcgtactgc tcggtcagtt ctacggcgtc
     1981 attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt
     2041 gggattggca gcattccggg agcgatgatt ggcgggctga ttctggggat tgcggaggcg
     2101 ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc cctgctgatt
     2161 ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga
     2221 aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc ctggcgggcg
     2281 tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag
     2341 acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttt cagcttttgc
     2401 gacctgcttt ccagaaaggg ctgaaaagcg tttccggacc gaagtttatt ctgcctgcaa
     2461 ttgatggctc cacggtgaag caaaagctgt tcctcgtggc gctgctggtg cttgcggtgg
     2521 cgtggccgtt tatggtgtcg cgcgggacgg tggatatcgc caccctgacc atgatctaca
     2581 ttattctcgg tctcgggctg aacgtggttg ttggtctttc tggtctgctg gtgctggggt
     2641 acggcggctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac tattacggcc
     2701 tgggcttctg gacctgtctg ccgattgccg ggttaatggc cgcggcggcg ggcttcctgc
     2761 tcggctttcc ggtgcttcgc ttgcgcggtg actatctggc gatcgttacc ctcggtttcg
     2821 gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc ccgaacggaa
     2881 tcagtcagat cccgaaaccg actttcttcg gccttgagtt cagccgtacc gcccgtgaag
     2941 gcggttggga cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgcgtca
     3001 tcttcctgta tctggtggcg ttgctgctgg tggtactaag cctgtttgtc attaaccgcc
     3061 tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagatgag atcgcctgcc
     3121 gttcgctggg cttaagcccg cgtcgtatca agctgaccgc ttttaccatc agtgccgcgt
     3181 ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc ccggaatcct
     3241 tcacctttgc tgaatcggcg tttgtactgg cgatagtggt gctcggcggt atgggatcgc
     3301 agtttgcggt gattctggcg gcaattttgc tggtggtatc gcgcgaattg atgcgtgatt
     3361 tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc
     3421 cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag
     3481 gagagcaggc atgagtcagc cattattatc tgtgaacggc ctgatgatgc gcttcggcgg
     3541 cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg tctcgttaat
     3601 cggccctaac ggtgccggaa aaaccacggt ttttaactgt ctgaccggat tctacaaacc
     3661 caccggcggc accattttac tgcgcaatca gcacctggaa ggtttaccgg ggcagcaaat
     3721 cgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgttccgtg aaatgacggt
     3781 gattgaaaac ctgctggtgg cgcagcatca acaactgaaa accgggctgt tctctggcct
     3841 gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg ccgccacctg
     3901 gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc tggcctatgg
     3961 tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga ttttaatgct
     4021 cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg agctgattgc
     4081 cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata tgaagctggt
     4141 gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg caaacggtac
     4201 gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga
     4261 tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa atccaggcgc
     4321 tgcatgaggt gagcctgcat atcaatcagg gcgagattgt caccctcatt ggcgcgaacg
     4381 gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgct accagcgggc
     4441 gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag
     4501 cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc
     4561 tggcgatggg cggctttttt gccgaacgcg accagttcca ggagcgcata aagtgggtgt
     4621 atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcaggcacc atgtccggcg
     4681 gtgagcaaca aatgctggcg attggtcgcg cgctgatgag caacccgcgt ttgctgctac
     4741 ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc gacaccatcg
     4801 agcagctgcg cgagcagggg aggactatct tcctcgtcga gcagaacgcc aaccaggcgc
     4861 taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc tccgataccg
     4921 gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg taataacacg
     4981 ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa aattgcctga
     5041 tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg cacgatcttg
     5101 taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgagtaat ctgtagagtt
     5161 tgattcagac cttctatttt tccgcttatc cgtgccccat ctcccatttt ccctcaccca
     5221 cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt aacaaaaaag
     5281 ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg cgtgaattcg
     5341 cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca gcttcagcac
     5401 tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt ccgttctggc
     5461 attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt tttaacgccg
     5521 aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag aatttaagcg
     5581 cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt tatgaagttg
     5641 gcaccgccac catgatggca tcgaaagcca ttaaaccggt atatgacgtg tttaaagagg
     5701 cggggattca attcgatgag tcgcagtttg tgccgacggt ttccggttac tactccgaca
     5761 gcaaaacggg ccacttactc tcccagccat tcaacagctc gacccccgtt ctctattaca
     5821 acaaagacgc cttcaagaaa gcaggattag acccggaaca accgccgaaa acctggcagg
     5881 atctggcgga ctatgccgcg aaactgaaag cctccggtat gaagtgcggc tacgccagcg
     5941 gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg ccgtttgcca
     6001 gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag ccggagcagg
     6061 tgaaacacat cgccatgctc gaggagatga acaagaaggg cgacttcagc tacgtcggtc
     6121 gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc accgcctctt
     6181 ccggttctct tgccaacatt cgcgagtacg ccaaatttaa ttacggcgta ggcatgatgc
     6241 cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga gccagcctgt
     6301 gggtgatgca gggtaaagat aaagaaacgt ataccggtgt ggcgaagttc ctcgatttcc
     6361 tcgcgaagcc agaaaacgct gccgagtggc atcagaaaac cggttatctg ccaattacca
     6421 aagcggcgta tgacctgacc cgtgagcagg gcttttacga gaaaaaccca ggtgcggata
     6481 ccgcgacgcg tcagatgctg aataagccgc cgttgccgtt caccaaaggg ctgcgtctgg
     6541 gcaacatgcc gcagatccgc gtgattgtgg atgaagagct ggagagcgtg tggaccggta
     6601 agaagacacc acagcaggca ctggataccg ccgttgagcg tggaaatcag ttgctgcgcc
     6661 gctttgagaa atcgacgaag tcttaatcag tgtaatgtcg gatgcgtttc gcttatctga
     6721 cctggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca ggaattaacc
     6781 gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct gctggtcgcg
     6841 ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc gttgtggtac
     6901 tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggcct ggataacttc
     6961 gtcgcgctgt ttcatgacag ctactatctc gactccttct ggacgacgat aaaattcagc
     7021 acctttgtca ccgtcagcgg tttgctggtg tcgctgttct tcgcggcgct ggtggagtac
     7081 atcgtgcgcg gcagccgttt ctatcaaacc ttaatgctgc tgccttacgc cgtggctccc
     7141 gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct gatcacccat
     7201 tttctcgcgg agttcggcta cgactggaac cacgcgcaaa acagcggtca ggcaatgttt
     7261 ctggtggtgt ttgcctcagt atggaagcaa atcagctaca acttcctgtt cttctatgcc
     7321 gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg tgcaggccca
     7381 attcgccgct tctttaagat tgcgctgccg cttatcgctc cggtgagttt cttcctgctg
     7441 gtggtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc caccacgtcc
     7501 ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgtga aggctttacc
     7561 ggactggatc tggcttcgtc tgccgcgcag tcggtggtgt tgatgttcct cgtcatcgtg
     7621 ctgacggtgg tgcagttcct ctatgttgaa agcaaggtgc gttaccaatg attgagaacc
     7681 gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg gtgatcctct
     7741 tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc tatgccgcgc
     7801 cgatgacgct catccccggc acacatctgc tggaaaatat ccacaacatc tgggttaacg
     7861 gggtaggcac gaatagcgcg ccgttctggc ggatgttgct taacagcttt gtgatggcgt
     7921 tcagcattac gctcggcaaa attaccgtct cgatgctctc ggcatttgcc attgtctggt
     7981 ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg atgctgccgg
     8041 ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgaagatg ctcgacagct
     8101 acgccggttt aacgctgccg ctgatggcct cggcgaccgc cacgtttctg tttcgccagt
     8161 tctttatgac gctgccggat gaactggtgg aagcggcgcg gatcgacggt gcgtcaccaa
     8221 tgcgcttctt ttgcgacatc gtttttccgc tctccaaaac taatctggcg gcgctgtttg
     8281 tgatcacctt tatttacggc tggaaccagt atttgtggcc gttgttgatt attaccgacg
     8341 tggatctcgg caccaccgtg gcagggatca aagggatgat cgccactggc gaaggcacca
     8401 cggaatggaa ctcagtgatg gcggcgatgt tgttaaccct gatcccacca gtagtgattg
     8461 ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa taagatggca
     8521 ggactgaaat tacaggcagt aaccaaaagc tgggatggca aaacccaggt cattaaaccg
     8581 ctgacccttg atgtggcaga tggcgaattt atcgtgatgg tcgggccgtc tggctgcggg
     8641 aaatcgacgc tgctgcgcat ggttgccggg ctggagcggg tgaccgaagg cgatatctgg
     8701 atcaacgacc agcgcgtgac cgaaatggag ccgaaagatc gcgggattgc gatggtgttc
     8761 cagaactacg cgctttatcc gcatatgagt gttgaagaaa acatggcgtg ggggctgaaa
     8821 atttgcggca tgggcaagca gcaaattgcc gagcgcgtta aagaagcggc gcgcattctg
     8881 gaactggacg gtctgcttaa gcgtcgcccg cgtgagctat ccggcggtca gcgccagcgt
     8941 gtggcgatgg gccgcgcgat tgtgcgcgat ccggcggtgt tcttgtttga tgagccgctc
     9001 tctaacctcg atgccaagct gcgcgtgcag atgcgtcttg aactgcaaca gttgcaccgt
     9061 cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaagcgat gacgctcgcc
     9121 cagcgagtaa tggtgatgaa cggcggcgtt gccgaacaga ttggcacacc agttgaagtc
     9181 tacgaaaagc ccgccagcct gtttgtggcg agttttatcg gcagcccggc gatgaacctg
     9241 ctgacaggcc gcgtgaataa tgaaggcacg catttcgaac tggacggcgg tatcgtgcta
     9301 ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgactctcgg cattcgcccg
     9361 gaacatatag cgctaagctc gcaggcagaa ggtggtgtgc cgctggtgat ggacacgctg
     9421 gagatcctcg gcgcagataa cctggcgcac ggacgctggg gcgagcagaa gctggtggtg
     9481 cgactggcgc atcaggagcg cccgacggca ggcagcacgc tgtggctgca tttggcggaa
     9541 aatcagctgc atctttttga tggtgaaaca ggacaacgag tatgagtaac tggccttatc
     9601 cccgcatcgt cgctcatcgt ggcggcggta agctggcccc ggaaaacacc ctggcggcaa
     9661 tcgacgtcgg ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag
     9721 atggcgagat cttcctgctc catgacgaca atctcgaacg caccagcaac ggctggggcg
     9781 tcgcgggtga actgaactgg caggatttac tgcgcgtgga tgcgggcagt tggtacagca
     9841 aagcgtttaa aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac
     9901 acgggatgat ggcgaatatc gaaatcaaac ccaccactgg cactggacca ttaacgggca
     9961 aaatggtggc gctggcggca cgccaactgt gggccggtat gacgccgccg ctgctgtcat
    10021 cgtttgagat tgatgcttta gaagctgcac aacaggcggc accggaactg ccgcgtggtt
    10081 tattgcttga tgagtggcgc gacgactggc gcgaactgac cgcgcggctg ggctgcgtct
    10141 ctattcatct caatcataag ttactcgata aagcgcgagt gatgcagttg aaagacgccg
    10201 gattacggat tctggtttat accgtcaata aaccccagcg cgcggcagag ttgctgcgct
    10261 ggggcgtgga ttgcatctgt accgatgcga ttgacttgat tggtccgaac tttacggccc
    10321 aatagttttc aacggaatat caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt
    10381 tctcctttcc ggcagcatat gctgctgatt caacgaacta tccggattac ggttgctgtt
    10441 taacatcccg ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc gctcccccgg
    10501 ctgtgactgc aacacccgct gagaattatt gtttatctgg ttttctaaat gctgctgttg
    10561 caactgagtt tgcgttttca gttgctgatt cagcatccct ttttgctgga tttgctgagt
    10621 ctgcatctgg gtttgcatcc gctgctggct gggtatctga taccccggct ggttagggtt
    10681 gttcagagta ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg taagaatcag
    10741 aagtcgtttc atcgcgtatc ctcctctgaa gatatccttt aagtttactc gcttcccgac
    10801 aaaacgatga ttaattcaga gttatatacc aggcttagct ggggttgccc cttaatctct
    10861 ggagaataac gatgataaaa ccgacgtttt tacgccgggt ggccattgct gctctgctct
    10921 caggaagttg ttttagcgcc gccgccgcgc ctcctgcgcc gcccgtctcg tatggtgtgg
    10981 aggaagatgt cttccacccg gtacgcgcga aacagggaat ggtagcgtct gtggacgcca
    11041 ctgccactca ggtgggggtg gatattctca aggagggcgg gaatgccgtt gatgccgccg
    11101 tggcggtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg ggcggtggtg
    11161 gttttatgtt aatccgctcg aaaaatggca ataccaccgc tatcgatttc cgcgaaatgg
    11221 cacccgccaa agcgacccgc gatatgttcc tcgatgatca gggcaacccg gacagcaaaa
    11281 aatcactcac ttcgcatctg gcttccggca caccgggtac ggtagcaggt ttctcgctgg
    11341 cgctggataa atacggcacc atgccgctga acaaagtcgt gcagcccgcg tttaaactgg
    11401 cacgcgatgg ttttatcgtt aacgacgcgc tggctgacga tctcaaaacc tacggtagcg
    11461 aagtgttgcc gaatcacgaa aacagtaaag ctatcttctg gaaagagggc gagccgctga
    11521 aaaagggcga cacgctggtg caggcgaacc tggcaaagag cctggagatg attgctgaaa
    11581 acggcccgga cgaattctat aaaggcacga ttgcggaaca gatcgcccag gagatgcaga
    11641 aaaacggtgg cttgatcact aaagaagatt tagcggccta taaagcggtc gaacgcactc
    11701 cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca tcctccggcg
    11761 ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag aaatacggct
    11821 ttggcagcgc cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc tacgccgacc
    11881 gctcggaata tcttggcgac ccggattttg tcaaagtccc gtggcaggca ctgaccaata
    11941 aagcctatgc taaatccatt gccgatcaaa ttgatatcaa caaagcgaag ccgtccagtg
    12001 agattcgccc tggcaagctt gcgccttatg agagtaatca aactacccat tactctgtag
    12061 tggataaaga cggtaacgcg gtggcggtga cctatacgct gaacaccacc ttcggtacgg
    12121 gtattgtcgc gggcgagagc ggtattctgc ttaataacca gatggatgat ttctccgcca
    12181 aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc gtcgggccga
    12241 acaaacgccc gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt aaaacctggc
    12301 tggttaccgg tagcccaggc ggtagccgga tcatcactac agtgctgcaa atggtggtga
    12361 atagcatcga ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc
    12421 agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg ctcaagctgc
    12481 tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca caaagcatta
    12541 tggttgggcc ggacggtgag ttgtacggcg catccgaccc gcgctcggtg gatgatttaa
    12601 cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctatttt gtcagggcaa
    12661 gccgaaggta gcctttttta tttcgtaatc ctgtagatat tcttccagcg gctttgccgt
    12721 tcccagagaa tgaatttcac cactgtcttt atcaataata aaaggtgcgt taccagctaa
    12781 gcgcgcggcc tcatctccag tttcgagaaa ttctcgtgct tcgaaacaga aataccagcc
    12841 ctggctaaag cgtccatgta gagtaatgac gaccgggaga tctgcatcat caaggtaatg
    12901 gttcgctttc gcgaatgcgt cgtgataagt aatcataatt ataataaata ttattgttga
    12961 gtgttatatt attattcctg tgtgatacat tgagcaaaga cgcgttcatc ttcgtcaaag
    13021 atattattaa atgctggttt tga
//
LOCUS       DAZFAH010000131        13014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFAH010000131 DAZFAH010000000
VERSION     DAZFAH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.131"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..1512)
                     /gene="xylB"
                     /locus_tag="ACHJWB_003374"
     CDS             complement(58..1512)
                     /gene="xylB"
                     /locus_tag="ACHJWB_003374"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4314949.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1584..2906)
                     /gene="xylA"
                     /locus_tag="ACHJWB_003375"
     CDS             complement(1584..2906)
                     /gene="xylA"
                     /locus_tag="ACHJWB_003375"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4314950.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3272..4264
                     /gene="xylF"
                     /locus_tag="ACHJWB_003376"
     CDS             3272..4264
                     /gene="xylF"
                     /locus_tag="ACHJWB_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4314951.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKSITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4342..5883
                     /gene="xylG"
                     /locus_tag="ACHJWB_003377"
     CDS             4342..5883
                     /gene="xylG"
                     /locus_tag="ACHJWB_003377"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4314952.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5861..7042
                     /gene="xylH"
                     /locus_tag="ACHJWB_003378"
     CDS             5861..7042
                     /gene="xylH"
                     /locus_tag="ACHJWB_003378"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4314953.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVMGLLLGAWNGWWVAYRKVPSFIVTLAGILAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7120..8298
                     /gene="xylR"
                     /locus_tag="ACHJWB_003379"
     CDS             7120..8298
                     /gene="xylR"
                     /locus_tag="ACHJWB_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4314954.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8406..9230)
                     /gene="bax"
                     /locus_tag="ACHJWB_003380"
     CDS             complement(8406..9230)
                     /gene="bax"
                     /locus_tag="ACHJWB_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4314955.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9550..11580
                     /gene="malS"
                     /locus_tag="ACHJWB_003381"
     CDS             9550..11580
                     /gene="malS"
                     /locus_tag="ACHJWB_003381"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4314956.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11758..13011
                     /gene="avtA"
                     /locus_tag="ACHJWB_003382"
     CDS             11758..13011
                     /gene="avtA"
                     /locus_tag="ACHJWB_003382"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4314957.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
BASE COUNT         3318 a         3282 c         3208 g         3206 t
ORIGIN      
        1 attagtgaga tatatagatg tgaattatcc cccacccggt caggcagggg ataacgttta
       61 cgccattaat ggcagaagtt gctgatagag gcgacggaac gtttcacgtc gtggctgata
      121 agcggcataa cgctgcgcat ctggtagatg cgactgttct aacggtagct gcggcaacaa
      181 ctcaatgagc gatttctctg gattcgccgc aatctgcgcc agccttgctg cgcccagtgc
      241 tggccccaca tccccccccg tacggtaatc gagctgctga ccgctgatat ccgccagcat
      301 ctgacgccag tactcactac gcgccccgcc accaatcaac gtaacacttt gcggtttaat
      361 gccgcaggca tgcacgacat ccatgccatc tgccagcgca taacccacgc cttccagcac
      421 tgctcgcgcc agttcattgg ggccatgttg atgagtcaaa ccaaagaaaa cccccttcgc
      481 ctggggatta ttgtgtggtg tacgctcgcc ggaaagataa ggcagaaacc aaactggctc
      541 ggcactttca tcagcctgtt gagctgcagc gattaaagct gggacattgc acaggccggt
      601 taatttcgcg acccaatcca gacacgatgc tgcactcagc attacagaca ttaaatgcca
      661 acgctgcggt agcgcatggc aaaagctgtg tacagcgctt tctggcttgc ttaagaaccc
      721 ttcgctgaca gcaaaataga cccccgacgt ccccagcgat aacattgcct gattagcatc
      781 aaccattccc acaccaactg caccagctgc attgtcgcca ccgcctgcaa caactggcac
      841 cgtcgccata ccccacgctt tcgcaacttc aggtaacaaa gcaccagtaa tttcgctgcc
      901 ttcgtataat gcgggcatct ggtcacgaga taagtcgcaa gcctgcagca tgacgtcact
      961 ccagtcacgc tttgcgacat ccagccacat ggtgccagct gcgtcagaca tatcgctggc
     1021 aaactccccc gtcatacgca gacgcaagta atctttcggt aataatactt tgtcgatttg
     1081 acggaatatc tccggctcat gccgctgaac ccatagcaat ttaggcgcag taaatccggg
     1141 catcatcagg ttgccggtaa tcactcgtga ttgcggaact ctcgcttcca gcaaagtgca
     1201 ctcttgcgca cagcgcccgt cgttccacaa aatggcaggg cgcaataccc gttgttgagc
     1261 atccagtaag gttgctccat gcatctggcc ggcaataccc aatgctttaa cgtcctgcag
     1321 agaatgctga tcgcccagag ctttcattgc gcgatcagtt gcctgccacc actgttccgg
     1381 gtcttgttcc gaccagagtg gatgcgggcg cgaaacggtc agcttttccg tttgcgaagc
     1441 aaccacctca ccctgctcgt tgagcaaaat aacttttacg cccgaggtgc caagatctat
     1501 cccgatatac atatcgatcg ttccttaaaa aaatgcccgg tatcgctacc gataaccggg
     1561 ccaacggact gcacagttag ccgttatttg tcgaacagat aatgatttac cagattttcc
     1621 agttgctcct ggcgaccact ctgatgcacc ggagacaaat tatgttcctg agcatatttg
     1681 gctaaatctg ccagtgacat ttggcctttc aggatttgct ggcccaattc gctattccag
     1741 ccggaataac gctgcgcgat gcgtttatcc agctcgccat cttcaatcat gcgcgctgca
     1801 attttcagcg ccagtgccat cgtatccatc gcgccgatat gaccgtaaaa cagatcatat
     1861 ttatcagtac tttgacgacg tactttggca tcgaagttca aaccaccggt ggtgaaaccg
     1921 cctgctttga gaatttcata catcaccagc gcattctctt ccacactgtt cgggaactgg
     1981 tcggtgtccc agcccagttg cgcatcgcca cggttggcgt cgacagaacc gaacaggcca
     2041 agcgcaatgg cggtggctat ttcatgatgg aaagagtgac ctgccagcgt cgcgtggtta
     2101 gcttcaatgt tcagtttaat ctctttttcc agaccaaact gtttcaggaa gccatagacc
     2161 gtcgcggcat cgtaatcata ttgatgttta gtcggttctt gcggtttcgg ttcgataagc
     2221 aacgtgccct ggaagccgat tttatgttta tgctcaacca ccatctgcat aaagcggccc
     2281 agttgttcac gctcctgacg caagtcggta tttaacagcg tttcgtaacc ttcacgacca
     2341 ccccacagga catagttttc accgcccaat ttatgggttg cttccatcgc tgtaacaact
     2401 tgcgttgccg cccagctgaa gacttcagga tctgggttcg tcgccgcacc cgcgccgtag
     2461 cgagggtttg taaagcagtt ggcggttccc cacagcagct tcacgccgct ctcttcttgc
     2521 ttgcctgcca ggacatcaac catttgcgca aaattattga tgtactcttt taacgacgcg
     2581 ccctcagggg aaacatccac atcgtggaag caataaaatg gcacatgtaa cttgtggaaa
     2641 aactcaaatg cgacatctgc tttacgcttc gccaacgcca gtgcctcacc aggctgctgc
     2701 cacggacgat taaacgcccc cacaccaaac atgtccgccc cgttccagca gaaggtgtgc
     2761 cagtagcagg cggcaaaacg caagtgctct tccatacgct tacccaacac cagttcgtcg
     2821 ggattgtagt gacggaatgc taacgggttt gaggattttg agccttcata acgaacgcga
     2881 tcgagctggt caaaataggc ttgcatattg aactccataa tcaggtaatg ccgcgggtga
     2941 tggatgatgt cgtaatattg ggcactccct ttcagttgct caattatgtt atttcacact
     3001 gctattgaga taattcacaa gtgtgcgctc gctcgcaaaa taaaatggga tgatgaaact
     3061 gggtaattcc tcgaagataa aaatgcaata agtacaattg cgcaacaaaa gtaagatctc
     3121 ggtcataaat caagaaataa accaaaaatc gtaatcgaaa gataaaaatc tgtaattgtt
     3181 ttcccctgtt tagttgctaa aaattggtta cgtttatcgc ggtgattgtt acttattaaa
     3241 gctgtcctct aactacagaa ggccctacac catgaaaata aagaacattc tactcaccct
     3301 ttgcacctca cttctgctta ccaacgttgc ggcacacgcc aaagaagtca aaataggtat
     3361 ggcgattgat gatctccgtc ttgaacgctg gcaaaaagat cgagatattt ttgtgaaaaa
     3421 ggcagaatct ctcggcgcga aagtatttgt acagtctgca aatggcaatg aagaaacaca
     3481 aatgtcgcag attgaaaaca tgattaacag gggtgtcgat gttcttgtca ttattccgta
     3541 taacggccag gtattaagta acgttgtaaa agaagccaaa caagaaggta ttaaagtatt
     3601 agcttacgac cgtatgatta acgatgcgga tatcgatttt tatatttctt tcgataacga
     3661 aaaagtcggt gaactgcagg caaaagccct ggtcgatatt gttccgcaag gtaattactt
     3721 cctgatgggc ggctcgccgg tagataacaa cgccaagctg ttccgcgccg gacaaatgaa
     3781 agtgttaaaa ccttacgttg attccggaaa aattaaagtc gttggtgacc aatgggttga
     3841 tggctggtta ccggaaaacg cattgaaaat tatggaaaac gcgctaaccg ccaataataa
     3901 caaaattgat gctgtagttg cctcaaacga tgccaccgca ggtggggcaa ttcaggcatt
     3961 aagcgcgcaa ggtttatcag ggaaagtagc aatttccggc caggatgcgg atctcgcagg
     4021 tattaaacgt attgctgccg gtacgcaaac tatgacggtg tataaatcta ttacgttgtt
     4081 ggcaaatact gccgcagaaa ttgccgttga gttgggcaat ggtcaggaac caaaagcaga
     4141 taccacactg aataatggcc tgaaagatgt gccctcccgc ctcctgacac cgatcgatgt
     4201 gaataaaaac aacatcaaag acacggtagt taaagacgga ttccacaaag agagcgagct
     4261 gtaagcgtta cgccccggcg cggagcgggg gcgtgatttc tctccatgcc gcgtgaatga
     4321 attggcttag gtggagtcgt tatgccttat ctacttgaaa tgaagaacat taccaaaacc
     4381 ttcggcagtg tgaaggcgat tgataacgtc tgcttgcggt tgaatgctgg cgaaatcgtc
     4441 tcactttgtg gggaaaatgg gtctggtaaa tcaacgctga tgaaagtgct gtgtggtatt
     4501 tatccccatg gctcctacga aggcgaaatt atttttgcgg gagaagagat tcaggcgagt
     4561 cacatccgcg ataccgaacg caaaggtatc gccatcattc atcaggaatt ggccctggtg
     4621 aaagaattga ccgtgctgga aaatatcttc ctgggtaacg aaataaccca caatggcatt
     4681 atggattatg acctgatgac gctacgctgt cagaagctgc tcgcacaggt cagtttatcc
     4741 atttcacctg atacccgcgt tggcgattta gggcttgggc aacaacaact ggttgaaatt
     4801 gccaaggcac ttaataaaca ggtgcgcttg ttaattctcg atgaaccgac agcctcatta
     4861 actgagcagg aaacgtcggt tttactggat attattcgcg atctacaaca gcacggtatc
     4921 gcctgtattt atatttcgca caaactcaac gaagtcaaag cgatttccga tacgatttgc
     4981 gttattcgcg acggacagca cattggtacg cgtgatgctg ccggaatgag tgaagacgat
     5041 attatcacca tgatggtcgg gcgagagtta accgcgcttt accctaatga accacatacc
     5101 accggagatg aaatattacg tattgaacat ctgacggcat ggcatccggt caatcgtcat
     5161 attaaacgag ttaatgatgt ctcgttttcc ctgaaacgtg gcgaaatact gggtattgcc
     5221 ggactcgttg gtgccggacg taccgagacc attcagtgcc tgtttggcgt gtggcccgga
     5281 caatgggaag gaaaaattta tattgatggc aaacaggtag atattcgtaa ctgtcagcaa
     5341 gccatcgccc aggggattgc gatggtaccc gaagacagaa agcgcgacgg catcgttccg
     5401 gtaatggcgg ttggtaaaaa tattaccctc gccgcactca ataaatttac cggtggcatt
     5461 agccagcttg atgacgcgga agagcaaaaa tgtattctgg aatcaattca gcaactcaaa
     5521 gttaaaacgt cgtcccccga tcttgctatt ggacgtttga gcggcggcaa tcagcaaaaa
     5581 gcgatcctcg cccgctgtct gttactcaac ccgcgcattc tcattcttga tgaacctacc
     5641 aggggtattg atattggcgc gaaatatgag atctacaaat taattaacca actcgtccag
     5701 cagggtattg ccgttattgt catctcttcc gaattacctg aagtgctcgg ccttagcgat
     5761 cgtgtactgg tgatgcatga agggaaacta aaagccaacc tgataaatca taacctgact
     5821 caggagcagg tgatggaagc cgcattgagg agcgaacatc atgtcgaaaa gcaatccgtc
     5881 tgaagtgaaa ttggccgtac cgacatccgg tggcttttct gggctgaaat cactgaattt
     5941 gcaggtcttc gtgatgattg cagctatcat cgcaatcatg ctgttcttta cctggaccac
     6001 cgatggtgcc tacttaagcg cccgtaacgt ctccaacctg ttacgccaga ccgcgattac
     6061 cggcatcctc gcggtaggaa tggtgttcgt cataatttct gctgaaatcg acctttccgt
     6121 cggctcaatg atggggctat taggtggcgt cgcggcgatt tgtgacgtct ggttaggctg
     6181 gcctttgcca cttaccatca ttgtgacgtt ggttatggga ctgcttctcg gtgcctggaa
     6241 cggatggtgg gtcgcgtacc gcaaagtccc ttcatttatt gtcaccctcg cgggcatttt
     6301 ggcatttcgc ggcatactca ttggcatcac caacggcacg actgtatccc ccaccagcgc
     6361 cgcgatgtca caaattgggc aaagctatct ccccgccagt accggcttca tcattggcgc
     6421 gcttggctta atggcttttg ttggttggca atggcgcgga agaatgcgcc gtcaggcttt
     6481 gggtttacag tctccggcct ctaccgcagt agtcggtcgc caggctttaa ccgctatcat
     6541 cgtattaggc gccatctggc tgttgaatga ttaccgtggc gttcccactc ctgttctgct
     6601 gctgacgttg ctgttactcg gcggaatgtt tatggcaacg cggacggcat ttggacgacg
     6661 catttatgcc atcggcggca atctggaagc agcacgtctc tccgggatta acgttgaacg
     6721 caccaaactt gccgtgttcg cgattaacgg attaatggta gccatcgccg gattaatcct
     6781 tagttctcga cttggcgctg gttcaccttc tgcgggaaat atcgccgaac tggacgcaat
     6841 tgcagcatgc gtgattggcg gcaccagcct ggctggcggt gtgggaagcg ttgccggagc
     6901 agtaatgggg gcatttatca tggcttcact ggataacggc atgagtatga tggatgtacc
     6961 gaccttctgg cagtatatcg ttaaaggtgc gattctgttg ctggcagtat ggatggactc
     7021 cgcaaccaaa cgccgttctt gattttgata aaaattttct caaagccggt tacgtattac
     7081 cggttttgag tttttgcatg attcagcagg aaaagaacca tgtttactaa acgtcaccgc
     7141 atcacattac tgttcaatgc caataaagcc tatgaccggc aggtagtaga aggcgtaggg
     7201 gaatatttac aggcgtcaca atcggaatgg gatattttca ttgaagaaga tttccgcgcc
     7261 cgcattgata aaatcaagga ctggttagga gatggcgtca ttgccgactt cgacgacaaa
     7321 cagatcgagc aagcgctggc tgatgtcgac gtccccattg ttggggttgg cggctcgtat
     7381 caccttgcag aaagttaccc acccgttcat tacattgcca ccgataacta tgcgctggtt
     7441 gaaagcgcat ttttgcattt aaaagagaaa ggcgttaacc gctttgcttt ttatggtctt
     7501 ccggaatcaa gcggcaaacg ttgggccact gagcgcgaat atgcatttcg tcagcttgtc
     7561 gccgaagaaa agtatcgcgg agtggtttat caggggttag aaaccgcgcc agagaactgg
     7621 caacacgcgc aaaatcggct ggcagactgg ctacaaacgc tgccaccgca aaccgggatt
     7681 attgccgtta ctgacgcccg agcgcggcat attctgcaag tatgtgaaca tctacatatt
     7741 cccgtaccgg aaaaattatg cgtgattggc atcgataacg aagaactgac ccgctatctg
     7801 tcgcgtgtcg ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta tcaggcggca
     7861 aaactgttgc atcgattatt agataaagaa gaaatgccgc tacagcgaat tttggtccca
     7921 ccagttcgcg tcattgaacg gcgctcaaca gattatcgct cgctgaccga tcccgccgtt
     7981 attcaggcca tgcattacat tcgtaatcac gcctgtaaag ggattaaagt ggatcaggta
     8041 ctggatgcgg tcgggatctc gcgctccaat cttgagaagc gttttaaaga agaggtgggt
     8101 gaaaccatcc atgccatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt
     8161 tcaaccacct tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat
     8221 ttctactctg tttttaaaaa agcatatgac acaacgccaa aagagtatcg cgatgtaaat
     8281 agcgaggtca tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac
     8341 agccacctga tgcgagcaaa agccttatct gaccgacaaa tcagataagg ctcaaggaaa
     8401 tgcaattaca tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag
     8461 ttattataac tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca
     8521 gtcacttcct gatccgcttt acgcagctgc gcacgcgatt tacggaacga agagtaagcc
     8581 gggtgcgtat tcaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt
     8641 gagtaccctt tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg
     8701 aacagattgt tattgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc
     8761 atcgtcgcca ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg
     8821 atttttcgcg tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc
     8881 gcacgctcag caggtgacca ttggccctga tactgttttg aaatcagcca gttacgttct
     8941 gcagtaatgg ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt
     9001 ttccttggtg ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta
     9061 ttgcgagaat actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac
     9121 ttttgactcg ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg
     9181 agtaacataa gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct
     9241 ggtcggattt aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa
     9301 atcatatcaa aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt
     9361 cacgtgttaa cgattcagat tggcgctaaa tcgcagaaaa tgtggggttt atcgcaaaat
     9421 tcagccgttt tttgcgcgag atcgctcacc cttgcttctc atcctgtgga cttaccgctc
     9481 agggatgagt tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac
     9541 tcatccacca tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc
     9601 gccagctgga cttctccggg gttccctgcc tttagcgaac agggaacggg aacatttgtc
     9661 agccacgcgc agttgcccaa aggtacgcgt ccactcacgc taaattttga ccaacagtgc
     9721 tggcagcctg cagatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac
     9781 acgccgcctc aatggcgatt gttcagagac ggcaaatata cgctgcaaat agacacccgc
     9841 tccggtacgc caacattgat gatttccatc cagaacgccg ccgaaccggt agcaaacctg
     9901 gtccgtgaat gcccgaaatg ggatggatta ccgctcacgc tggatgtcag cgccactttc
     9961 ccggaaggag ccgccgtacg ggattattac agccagcaaa ttgcgatagt gaagaacggt
    10021 caaataacgt tacaacccgc tgctaccagc aacggtttac tcctgctgga acgggcagaa
    10081 actgacgccc ctgccccttt cgactggcat aacgccacgg tttactttgt gctgacagat
    10141 cgtttcgaaa acggcgatcc cagtaatgac cagagttacg gacgtcataa agacggtatg
    10201 gcggaaattg gcacttttca cggcggcgat ttacgcggcc tgaccaacaa actggattac
    10261 ctccagcagt tgggcgttaa tgctttatgg ataagcgccc catttgagca aattcacggc
    10321 tgggtcggcg gcggtacaaa aggcgatttc ccgcattatg cctaccacgg ttattacaca
    10381 caggactgga cgaatcttga tgccaatatg ggcaacgaag ccgatctacg gacgctggtt
    10441 gatagcgcac atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa ccacaccggc
    10501 tatgccacgc tggcggatat gcaggagtat cagtttggcg cgttatatct ttctggtgac
    10561 gaggtgaaaa aaacgctggg tgaacgctgg agcgactgga aacctgccgc cgggcaaacc
    10621 tggcatagct ttaacgatta cattaatttc agcgacaaaa caggctggga taaatggtgg
    10681 ggaaaaaact ggatcagaac ggatatcggc gattacgaca atcctggatt cgacgatctc
    10741 actatgtcgc tggccttttt gccggatatc aaaaccgaat caactaccgc ttctggtctg
    10801 ccggtgttct ataaaaacaa aacggatact cacgctaaag tcatcgaagg ctttacacct
    10861 cgcgattact taacccactg gttaagtcag tgggtccgcg actatgggat tgatggtttt
    10921 cgggtcgata ccgccaaaca tgttgagttg cccgcctggc agcaactgaa aaccgaagcc
    10981 agcgccgcgc ttcgcgaatg gaaaaaagct aaccccgaca aagcattaga tgacaaacct
    11041 ttctggatga ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta ctatcgccac
    11101 ggcttcgatg cgatgatcaa tttcgattat caggagcagg cggcgaaagc agtcgattgt
    11161 ctggcgcaga tggatacgac ctggcagcaa atggcggaga aattgcaggg tttcaacgtg
    11221 ttgagctacc tctcgtcgca tgatacccgt ctgttccgtg aagggggcga caaagcagca
    11281 gagttattac tattagcgcc aggcgcggta caaatctttt atggcgatga atcctcgcgt
    11341 ccgttcggtc ctacaggttc tgatccgctg caaggtacac gttcggatat gaactggcag
    11401 gatgttagcg gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag ccagttccgc
    11461 gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac
    11521 ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtga
    11581 cttttccggc ttcccgttcg tcagtacctc gggaagccgc caaccaggat aaaatgtcag
    11641 ccctaatcag cgttgcagga taaagcaccg ctcactctcc aacagaccga tttgcacccc
    11701 agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg
    11761 acattctccc tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa
    11821 gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccggcg
    11881 cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc
    11941 aaagcgactg atgcactgtg caattacgac ggtccacagg ggaaaacgga gctactcaca
    12001 ctgcttgccg gaatgctgcg cgagaagttg ggttgggata tcgaagcaca gaatattgca
    12061 ctaacaaacg gcagccagag cgcgtttttc tacttattta atctgtttgc cggacgccgt
    12121 gccgatggtc gggtcaaaaa agtgctgttc ccgctggcac cggaatacat tggctacgct
    12181 gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga actgctgccg
    12241 gaaggccagt ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg
    12301 atgatttgcg tctcccggcc gacgaatcca acaggcaatg tgattactga cgaagagttg
    12361 ctgaagcttg acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat
    12421 ggcgtcccgt tcccgggtat catcttcagt gaagcgcgcc cgctatggaa tccgaatatc
    12481 gtgctgtgca tgagtctttc caagctgggg ctgcctggct cccgctgcgg cattatcatc
    12541 gccaatgaaa aaatcatcac tgccatcacc aatatgaacg gcattatcag cctggcacct
    12601 ggcggtattg gtccggcgat gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg
    12661 tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaactat cgccatcatt
    12721 cgccgctatt taccggaaga tcgctgccta attcataaac cagaaggagc cattttcctc
    12781 tggctatggt ttaaggattt gcccattacg accgagcaac tctaccagcg cctgaaagca
    12841 cgcggcgtgc tgatggtgcc ggggcacaac ttcttcccgg gactggataa accgtggccg
    12901 catacgcatc aatgtatgcg catgaactac gtaccagagc cggagaaaat tgaggcgggg
    12961 gtgaagattc tggcggaaga gatagaaaga gcctgggctg aaagtcacta aaaa
//
LOCUS       DAZFAH010000132        12930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFAH010000132 DAZFAH010000000
VERSION     DAZFAH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.132"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..420
                     /gene="yjdM"
                     /locus_tag="ACHJWB_003383"
     CDS             85..420
                     /gene="yjdM"
                     /locus_tag="ACHJWB_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4314958.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            578..1021
                     /gene="yjdN"
                     /locus_tag="ACHJWB_003384"
     CDS             578..1021
                     /gene="yjdN"
                     /locus_tag="ACHJWB_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4314959.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1154..1942
                     /gene="phnC"
                     /locus_tag="ACHJWB_003385"
     CDS             1154..1942
                     /gene="phnC"
                     /locus_tag="ACHJWB_003385"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4314960.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1967..2983
                     /gene="phnD"
                     /locus_tag="ACHJWB_003386"
     CDS             1967..2983
                     /gene="phnD"
                     /locus_tag="ACHJWB_003386"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4314961.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3089..3868
                     /gene="phnE"
                     /locus_tag="ACHJWB_003387"
     CDS             3089..3868
                     /gene="phnE"
                     /locus_tag="ACHJWB_003387"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4314962.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3889..4614
                     /gene="phnF"
                     /locus_tag="ACHJWB_003388"
     CDS             3889..4614
                     /gene="phnF"
                     /locus_tag="ACHJWB_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4314963.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4615..5067
                     /gene="phnG"
                     /locus_tag="ACHJWB_003389"
     CDS             4615..5067
                     /gene="phnG"
                     /locus_tag="ACHJWB_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4314964.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5064..5648
                     /gene="phnH"
                     /locus_tag="ACHJWB_003390"
     CDS             5064..5648
                     /gene="phnH"
                     /locus_tag="ACHJWB_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4314965.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGITEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5648..6712
                     /gene="phnI"
                     /locus_tag="ACHJWB_003391"
     CDS             5648..6712
                     /gene="phnI"
                     /locus_tag="ACHJWB_003391"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4314966.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6705..7550
                     /gene="phnJ"
                     /locus_tag="ACHJWB_003392"
     CDS             6705..7550
                     /gene="phnJ"
                     /locus_tag="ACHJWB_003392"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4314967.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7547..8305
                     /gene="phnK"
                     /locus_tag="ACHJWB_003393"
     CDS             7547..8305
                     /gene="phnK"
                     /locus_tag="ACHJWB_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4314968.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8482..9162
                     /gene="phnL"
                     /locus_tag="ACHJWB_003394"
     CDS             8482..9162
                     /gene="phnL"
                     /locus_tag="ACHJWB_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4314969.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9159..10295
                     /gene="phnM"
                     /locus_tag="ACHJWB_003395"
     CDS             9159..10295
                     /gene="phnM"
                     /locus_tag="ACHJWB_003395"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4314970.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10295..10852
                     /gene="phnN"
                     /locus_tag="ACHJWB_003396"
     CDS             10295..10852
                     /gene="phnN"
                     /locus_tag="ACHJWB_003396"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4314971.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10839..11273
                     /gene="phnO"
                     /locus_tag="ACHJWB_003397"
     CDS             10839..11273
                     /gene="phnO"
                     /locus_tag="ACHJWB_003397"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO4314972.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11275..12033
                     /gene="phnP"
                     /locus_tag="ACHJWB_003398"
     CDS             11275..12033
                     /gene="phnP"
                     /locus_tag="ACHJWB_003398"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4314973.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12180..12509)
                     /gene="yjdP"
                     /locus_tag="ACHJWB_003399"
     CDS             complement(12180..12509)
                     /gene="yjdP"
                     /locus_tag="ACHJWB_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4314974.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12578..12895)
                     /locus_tag="ACHJWB_003400"
     CDS             complement(12578..12895)
                     /locus_tag="ACHJWB_003400"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO4314975.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2823 a         3927 c         3648 g         2532 t
ORIGIN      
        1 gggattttgt gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc
       61 ctgctgactt ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa
      121 tacacttacg aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac
      181 gcagaacctg cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg
      241 gctgacggcg acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg
      301 ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat
      361 tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga
      421 ttgtattgtg atcggtaagc cggataaggc gctcgcgccg catccggcaa cgatgccgac
      481 tgcctgatgc gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac
      541 tacacttaac tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc
      601 ttttgccggt aactgtgccg acgcaattgc ttattatcaa cgtacgttgg gcgctgaact
      661 gctctataaa atcagcttcg gcgaaatgcc aaaatcagcg caggacagcg ccgagaactg
      721 cccttccgga atgcaatttc ccgataccgc catcgcccac gccaatgtgc gcattgccgg
      781 aagcgacatc atgatgagcg atgccatgcc gtcaggaaaa gccagctact ccggctttac
      841 gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc tggtttgaca atcttgccgc
      901 taacggaaaa atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt
      961 caccgataaa tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata
     1021 acccgccggg aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt
     1081 tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta
     1141 gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca
     1201 gcatcaggcg ctgcatgcgg ttgatctgaa cattcatcac ggtgaaatgg tggctctgct
     1261 tgggccgtcg ggttccggca aatccaccct tttacgtcac ttaagcggtt tgattaccgg
     1321 cgataaatcc gccggcagcc atatcgagct gctgggccgc acagtccagc gcgaaggccg
     1381 tctggcgcgc gatatccgca aaagccgcgc caacaccggc tacatcttcc aacaattcaa
     1441 cctggtgaac cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc
     1501 gttctggcgc acctgtttta gctggtttac ccgcgagcag aaacaacgcg cgttacaggc
     1561 gctgacccgc gttggcatgg tgcattttgc ccatcaacgc gtttccaccc tctccggcgg
     1621 acagcagcag cgtgtggcga ttgcccgcgc gctgatgcag caggcgaagg tgattctggc
     1681 cgatgaaccc atcgcctcgc tggacccgga atccgcccgc atcgtgatgg acaccctgcg
     1741 cgacatcaac cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc
     1801 cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg cacgttttct acgacggcag
     1861 cagccaacag tttgataacg aacgttttga ccatctctac cgcagcatta atcgcatcga
     1921 agagaacgcg aaagctgcct gacatcccca tcattgagga aaacgaatga acgctaagat
     1981 aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa gcccggcaca
     2041 cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa
     2101 cctgaaaccg caatggacgc cattcttaca ggatatggag aagaagctgg gcgtgaaggt
     2161 gaacgccttc tttgccccag actacgcagg cattatccag ggaatgcgct tcaataaagt
     2221 ggatatcgcc tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaacggtca
     2281 ggtcttcgct cagaccgtcg cggcggacgg atcgccgggt tactggagcg tgttgatcgt
     2341 caacaaagac agtccgatca acaacctgaa cgatctgctg gcgaagcgta aagatctcac
     2401 cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggct actacgtctt
     2461 cgccaaaaac aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac
     2521 caacgcgctg gccgtcgcca acaagcaggt ggatgttgcc actaacaaca ccgaaaacct
     2581 cgacaagctg aaaacctccg cgccggagaa gttgaaagaa ctgaaagtga tctggaagtc
     2641 gccgctgatc ccaggcgatc cgatcgtctg gcgtaaaaac ctttccgaaa ccaccaaaga
     2701 caagatctac gacttcttta tgaactacgg caaaacgccg gaagaaaaag cggtgctgga
     2761 acgcctgggc tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca
     2821 gctcgcgctg tttaaagaga tgcagggcgt gaaaagcaat aaaggactga atgagcagga
     2881 caagctggca aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa
     2941 cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag
     3001 acgccccggc gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc
     3061 ttatccggcc tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg
     3121 cagctggttc tcgcttctga gctgggccgt tgttctcgcc gtgttggtcg tctcgtggca
     3181 gggcgcggaa atggccccgc tcacgctgat taaagacggc ggcaacatgg cgacgttcgc
     3241 cgccgacttc ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt
     3301 cacgctgcaa atcgccgtct ggggcacggc gctggcggtg gttctctcca tccccttcgg
     3361 cctgatgagc gccgaaaacc tggtgccgtg gtgggtttac cagcccgttc gccgcctgat
     3421 ggacgcctgc cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg
     3481 cctcggcccg ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa
     3541 actgctttcc gaagcggtgg aagcgattga acccggcccg gtggaaggca ttcgcgccac
     3601 cggcgccaac aagctcgaag agatcctcta cggcgtgctg ccgcaggtga tgccgctgct
     3661 gatctcctac tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat
     3721 ggtcggtgca ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca
     3781 acaaacctgc gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc
     3841 tcaacggttg cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct
     3901 acacatccga ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag
     3961 cttcgtcaac actatcgctg cggcgactat cttcccgccg agcagcagct ggcggcgcgc
     4021 tttgaggtaa atcgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg
     4081 gtacagcgcc gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc
     4141 aacgcccagg cgcgttttag ccagaacctg ctggatcagg gcagccatcc caccagcgaa
     4201 aaactgctgt cggtgctgcg cccggcatct ggtcacgtcg ctgacgcgct ggggataacc
     4261 gagggggaga acgtcatcca cctgcgcacc ctgcgccggg tcaacggcat cgcgctctgt
     4321 ttaatcgacc actactttgc ggacctcacc ctctggccga cactgcaacg tttcgacagc
     4381 ggttcgctgc acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg
     4441 cggatcagcg cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg
     4501 tcgccgctgc tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag
     4561 tactccgtca gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac
     4621 gcagataccg cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa
     4681 ctggcagccc gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcactgcc
     4741 gaaactggcc tggtgcagat tcaggcgcga atgggcggta ccggcgaacg cttttttgcc
     4801 ggcgacgcca cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc
     4861 tgggtgctgg ggcgtgataa acagcacgcc gaacgctgcg cgctgattga tgcgctgatg
     4921 cagcaatctc gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac
     4981 cgtatggcac gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt
     5041 acgatggttc gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg
     5101 atgcccagca cagttttcgt cgcctgttaa aggccatgag cgagccgggc gtgattgtcg
     5161 ccctgcatca gctcaaacgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc
     5221 tgacgctggc cgataacgac acgccggtgt ggcttgccac gccattaagt aacgatatcg
     5281 tcaatcagag cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga
     5341 ccttcgcggt gacggatgag gcgatttcca gcgaacagct caacgccctt tccaccggca
     5401 ccgccgttgc gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg
     5461 ggcgcatgct gcgcctcacc ggcgcgggca ttaccgaaga acgaatgatc gctccgcagc
     5521 tgccggagtg cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg
     5581 acctgatcct gacctgcggc gagcgcctgc tggctgttcc gcgaaccact catgtggagg
     5641 tgtgctgatg tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct
     5701 gcaagagagc cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccagattga
     5761 acagcagctt aacctcgcgg tggatcgcgt gatgacagaa ggcggcattg ccgaccgcga
     5821 actggcggcg ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct
     5881 gcgcgcctac cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccacaga
     5941 gatgcgtctc gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct
     6001 tggcccaacc tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga
     6061 agcgccgacg ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct
     6121 gctggcgcgt caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat
     6181 cacccgcacg ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg
     6241 cggggacgaa ggctatttgc tggcgctggc ctactccacc cagcgtggtt acgggcgcaa
     6301 tcacccgttc gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga
     6361 gctgggattt gcggtaaacg tcggcgaact actgatgacc gagtgtgaaa tggtcaacgg
     6421 ttttatcgac ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg
     6481 catgagcgag cgcaaagcga tggcaatggc gctggtcgat cgtgcgttgc aggctccgga
     6541 atacggcgag cacgcgacag gcccggcgca ggatgaagag tttgtgctgg cacatgccga
     6601 caacgtcgaa gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca
     6661 ggccgaactg gagctactca aacgtctgca acaggagaag aaccatggct aatctgagcg
     6721 gctacaactt tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gctatcttaa
     6781 aagcggtggc gatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccat
     6841 acggctgggg aaccggcggc atacagctca ccgccagcgt gattggcgaa agcgacgtgc
     6901 tgaaggtgat tgaccagggt gcggatgaca ccaccaacgc cgtgtcgatt cgcaacttct
     6961 ttaagcgcgt gaccggggta aacaccactg aacgtacgga cgatgcgacg cttatccaga
     7021 cgcgtcaccg catccccgaa acgccgctga ccgaagatca gatcattatc ttccaggtgc
     7081 caatcccgga accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc accatgcacg
     7141 cgctggaaga gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc
     7201 atatcgccac tacctacgcc tatccggtga aggtgaacgg gcgctacgta atggacccgt
     7261 cgccgatccc gaaattcgat aacccaaaaa tggacatgat gcccgccctg caactgttcg
     7321 gcgcggggcg cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg
     7381 atttcgacga tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat
     7441 cgacccacag ctatcttgat gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct
     7501 gctccgatac cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt
     7561 actttcggtc aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc
     7621 ttttgattta tggccggggg aagtgctggg cattgtcggg gagtccggct ccgggaagac
     7681 cacgctgctg aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcactatga
     7741 gagccgttcg ctgtacgcaa tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg
     7801 gggcgtagtg catcagcatc cgctcgacgg cctgcgccgc caggtgtcgg caggcggcaa
     7861 tatcggcgag cggctgatgg cgaccggggc acgtcattac ggcgatattc gtgccaccgc
     7921 gcagaagtgg ctggaagagg tggaaattcc cgccaaccgg atcgacgacc tgccgaccac
     7981 cttttccggc ggcatgcagc aacgtttgca gatcgcccgc aacctggtga cgcatccgaa
     8041 gctggtgttt atggacgagc cgaccggcgg actggatgtg tcggtacagg cacgcctgct
     8101 cgacctgctg cgcggcctgg tggtggagct gaacctcgcg gtagtgattg tcacccacga
     8161 tttaggcgtc gcccgcctgc tggcggaccg tttgctggtg atgaagcagg ggcaagtggt
     8221 ggagagtggg ttaaccgacc gcgtgctcga cgacccgcac catccgtata cacaactgct
     8281 ggtgtcatcg gttttgcaga attgaatgca acaccgtgcg cgctgatccc ctcaccccgg
     8341 ccctctcccg gagggagagg gagaaaaccg gcgcaatatt caacaccgcg cacaatcggt
     8401 cccctcgccc cttcggggag agggttaggg tgaggggcaa tgcgcgcacc gctccccaat
     8461 atgcgaacca cgaggcgaaa aatgattaac gtacaaaacg tcagtaaaac cttcatcctg
     8521 caccagcaaa acggcgtgcg cctgcccgtc ttacaacgcg cctcgctcac cgtcaacgca
     8581 ggtgaatgcg tggtgctcca cggccattcc ggcagcggca aatcaactct gctacgctcg
     8641 ctgtacgcca actatctgcc cgacgaaggg caaatccaga tcaaacacgg tgacgaatgg
     8701 gtagacctgg tcaccgcgcc agcgcgcaaa gtggtggaga tccgcaaaac taccatcggc
     8761 tgggtgagcc agtttctgcg cgtcatcccg cgtatctcag cactggaagt ggtgatgcag
     8821 ccgctgctcg atacaggcgt tccgcgtgaa gcctgcgccg ctaaagccgc gcgtcttctc
     8881 acccgcctga acgtgccgga acgcctgtgg cacctggcac catcgacctt ctccggcggc
     8941 gaacagcagc gcgtcaacat cgcccgcggc tttatcgtcg actaccccat tctgctgctc
     9001 gacgaaccta ccgcctcgct ggacgccaaa aacagcgccg cggtggtgga actgattcac
     9061 gaagccaaag cccgtggcgc agccatcgtc ggcatcttcc atgacgaagc tgtacgtaat
     9121 gacgtcgccg accgcctgca cccaatggga gcctcttcat gattatcaat aacgttaagc
     9181 tggtgctgga aaacgaggtg gtgcacggtt cgctggaggt gcaggatggc gaaatccgcg
     9241 cctttgccga aagccagagc cgcctgccag aagcgatgga cggcgaaggt ggctggctac
     9301 tacccggcct gattgagctg cataccgaca acctcgataa attcttcacc ccgcgcccga
     9361 aagtcgactg gcccgcccat tcggcgatga gcagccacga cgcgctgatg gtggcaagcg
     9421 gcatcaccac cgtactggac gccgtggcga ttggtgatgt gcgcgacggc ggcgatcggc
     9481 tggagaatct ggagaagatg atcaacgcca tcgaagagac gcaaaaacgc ggcgtcaacc
     9541 gcgccgagca ccgcctgcac ctgcgctgcg aactgccgca tcacaccaca ctgccgctgt
     9601 ttgaaaaact ggttcagcgc gaaccggtga cgctggtgtc gctgatggac cactcaccgg
     9661 gccagcgcca gttcgctaac cgcgagaagt atcgcgaata ttatcagggc aaatactccc
     9721 tcaccgatgc gcagatgcag cagtacgaag aagagcaatt ggcgctcgcc gcacgctggt
     9781 cgcagccgaa tcgcgaatcc atcgccgccc tgtgccgcgc gcgacaaatt gcgctcgcca
     9841 gccacgatga cgccacgcac gcccacgttg ccgaatccca ccaacttggc agcgtgatcg
     9901 ccgaatttcc caccacgttc gaagcggcgg aagcctcgcg taagcatggc atgaacgtgc
     9961 tgatgggtgc gccaaatatc gtgcgtggcg gctcgcactc cggcaatgtg gcggccagtg
    10021 aactggcgca gcttggtctg ctggatatcc tctcttccga ctactacccc gccagcctgc
    10081 tggatgcggc gttccgcgtc gccgatgacg agagcaaccg ctttacgcta ccgcaggcgg
    10141 tgaggctggt gaccaaaaat ccggcgcagg cgctgaatct tcaggatcgc ggggtgattg
    10201 gcgagggtaa acgcgctgac ctggtgctgg cgcatcgcca gggcaatcac attcatatcg
    10261 accacgtctg gcgtcagggt aaaagggtgt tctgatgatg ggaaaactga tttggttaat
    10321 ggggccgtcc ggctccggga aagacagcct gctggcggaa ctccgcctgc gggaacaaac
    10381 gcagttactg gtggcgcatc gctacatcac gcgcgacgcc agcgccggaa gtgaaaacca
    10441 tatcgccctg agtgagcggg agttttttac ccgcgcgggg caaaacctgc tggccttaag
    10501 ctggcacgct aacggtctgt attatggcgt cggcatcgag attgacctct ggctgcacgc
    10561 tggattcgac gtagtcgtca acggttcacg cgcgcatttg ccgcaggcac gggcgcgcta
    10621 tcaatcggcg ctgctgcccg tctgtttaca ggtttcgccg gagatcctgc gccagcgcct
    10681 ggaaaaccgt ggccgtgaaa acgccagtga aattaacgcc cgcctggcgc gcgccgcccg
    10741 ctatactcca caggattgcc atacgctcaa caatgacggc agcctgcgcc agtcggtcga
    10801 cacgctgctg acgctgatcc atcagaagga gaaacaccat gcctgcttgt gagcttcgcc
    10861 cggccacgca gtacgatacc gacgcggttt acgcgctgat ttgtgagcta aaacaggcgg
    10921 agtttgacca ccatgcgttt cgcgtgggct ttaacgccaa cctgcgcgac ccaaacatgc
    10981 gctaccatct ggcgctgctg gatggcgaag ttgtcggcat gatcggcctg catttgcagt
    11041 ttcatctgca tcatgtcaac tggatcggcg aaattcagga actggtggtg atgccgcagg
    11101 cgcgcggtct gaacgtcggc agtaaattac tggcatgggc agaagaagaa gcccgccagg
    11161 ccggggccga aatgaccgag ctttccacca acgtgaagcg ccacgacgca caccgttttt
    11221 acctgcgtga aggctacgag cagagccact tccgcttcac caaggcgctg taacatgagc
    11281 ctgaccctca cgctcaccgg cactggcggc gcacagggcg ttccggcatg gggctgcgag
    11341 tgcgcggcct gcgccagagc gcggcgctcg ccgcagtatc gccgccagcc atgcagcggc
    11401 gtagtgaagt ttaacgacgc gatcaccctg atcgacgccg ggcggcacga tctcaccgat
    11461 cgctggtcgc ctggatcgtt ccagcagttt ttgctgacgc actaccatat ggatcacgtc
    11521 caagggctat ttccgctacg ctggggcgtg ggcgatgtga tcccggtcta cggcccgccg
    11581 gatgaacagg gctgcgacga tctgtttaaa catccgggcc tgcttgattt cagccacaca
    11641 gtggaaccgt ttgtggtgtt tgatttgcag gggttgcagg tcacgccgct gccgcttaac
    11701 cactcaaaac tgaccttcgg ttatctgctg gaaacggcac atagccgggt ggcgtggctg
    11761 tctgacaccg ctggcctgcc ggaaaaaacg ctgaaatttt tactcaataa tcatccgcag
    11821 gtgatggtga ttgattgcag tcacccgccg cgcgcggatg caccgcgtaa tcactgtgat
    11881 ttaaataccg tgcttgcgct gaatcaggtt atccgctcgc cacaggtgat tctgacccat
    11941 atcagccacc agtttgatgc gtggctgatg gaaaacgcac taccgtcagg gtttgaggtg
    12001 gggtttgatg ggatggagat tggggtggcg tgatgagagg gaatgtgcgc gctggccccc
    12061 tcaccctaac cctctcccca gaggggcgag gggaccgatt gtgctcaata ttgaatattg
    12121 cgctcgtttt ctccctctcc ccattggggt gaggggcgat gcctgctcca tacccaacct
    12181 catcgcccat actcatcttc cattctccgc tcttcatcct ccagttgccg acgctcctga
    12241 tcaagctggc gctggcgatc gtccagctgc ctgcggcgat cttcaaactg gcggcggcgg
    12301 tcgtcatatt gtctgcgccg atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa
    12361 tcttcatagt ctcgcccacg gtcagggtta taagcgtcat taatcgcctg ctgaatatta
    12421 ccaatagtat cgtcgataat atcagcctgg gccggaacgg tggacagcgt gagcagggtg
    12481 aataaaagaa atagcggaaa gcgtttcatt agccaacctc aaaaagaaac tctatccaca
    12541 ttagtcatta ctcatccatg caagtagtgg atgaatctca atttctccgc tgctctattg
    12601 ccgtaatcgc ctccacgcgt tgttgatgac gaccgccttc gtactgtgcg cccagccacg
    12661 catccacaat catttttgcc agttcgaggc caaccactcg tgaaccaaaa gccagtgcaa
    12721 cttcactggc ataatgcgga taatcagtgc gctctgacga cctggtgtct ttatcaatca
    12781 cctcactcat tgaccatgaa taaaaggctg gcacagcaac gcagaatcct gcggtgccag
    12841 cgacatgacc gcccattgct ccggtacgtt ttttaaaaag aaacaccggt tgcattgata
    12901 aattgtgctc agtatttgcc gaagtacaat
//
LOCUS       DAZFAH010000133        12924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFAH010000133 DAZFAH010000000
VERSION     DAZFAH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.133"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            290..871
                     /gene="mdaB"
                     /locus_tag="ACHJWB_003401"
     CDS             290..871
                     /gene="mdaB"
                     /locus_tag="ACHJWB_003401"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO4314976.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            902..1216
                     /locus_tag="ACHJWB_003402"
     CDS             902..1216
                     /locus_tag="ACHJWB_003402"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4314977.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1264..3156)
                     /gene="parE"
                     /locus_tag="ACHJWB_003403"
     CDS             complement(1264..3156)
                     /gene="parE"
                     /locus_tag="ACHJWB_003403"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4314978.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDKMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3185..3766)
                     /gene="yqiA"
                     /locus_tag="ACHJWB_003404"
     CDS             complement(3185..3766)
                     /gene="yqiA"
                     /locus_tag="ACHJWB_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4314979.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3766..4593)
                     /gene="cpdA"
                     /locus_tag="ACHJWB_003405"
     CDS             complement(3766..4593)
                     /gene="cpdA"
                     /locus_tag="ACHJWB_003405"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4314980.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQEQDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4618..5040)
                     /locus_tag="ACHJWB_003406"
     CDS             complement(4618..5040)
                     /locus_tag="ACHJWB_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4314981.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5041..5670)
                     /gene="nudF"
                     /locus_tag="ACHJWB_003407"
                     /pseudo
     CDS             complement(5041..5670)
                     /gene="nudF"
                     /locus_tag="ACHJWB_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
     gene            5875..7356
                     /gene="tolC"
                     /locus_tag="ACHJWB_003408"
     CDS             5875..7356
                     /gene="tolC"
                     /locus_tag="ACHJWB_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4314982.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7504..8175
                     /locus_tag="ACHJWB_003409"
     CDS             7504..8175
                     /locus_tag="ACHJWB_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4314983.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8181..9341
                     /locus_tag="ACHJWB_003410"
     CDS             8181..9341
                     /locus_tag="ACHJWB_003410"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4314984.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9379..10167)
                     /gene="ygiD"
                     /locus_tag="ACHJWB_003411"
     CDS             complement(9379..10167)
                     /gene="ygiD"
                     /locus_tag="ACHJWB_003411"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4314985.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     STPTPEHYLPLLYVLGAWDWQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10310..11083
                     /gene="zupT"
                     /locus_tag="ACHJWB_003412"
     CDS             10310..11083
                     /gene="zupT"
                     /locus_tag="ACHJWB_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4314986.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11141..11311)
                     /gene="yqiD"
                     /locus_tag="ACHJWB_003413"
     CDS             complement(11141..11311)
                     /gene="yqiD"
                     /locus_tag="ACHJWB_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4314987.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11573..12226)
                     /gene="ribB"
                     /locus_tag="ACHJWB_003414"
     CDS             complement(11573..12226)
                     /gene="ribB"
                     /locus_tag="ACHJWB_003414"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4314988.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12330..12478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12600..12890
                     /gene="ubiK"
                     /locus_tag="ACHJWB_003415"
     CDS             12600..12890
                     /gene="ubiK"
                     /locus_tag="ACHJWB_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4314989.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3267 a         3454 c         3155 g         3048 t
ORIGIN      
        1 taattatcaa catgacacgt tgatattttc atcttttact tattcaaagc catattttca
       61 atcccaaaaa aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg
      121 aatggctatc agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct
      181 agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc
      241 gctctgtgac gtactatagt cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat
      301 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
      361 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc
      421 cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
      481 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
      541 tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc
      601 gtcgaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctaac
      661 ctggaacgca ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggcgt
      721 tgacggtgtg tatctgccgt tccataaagc aaaccaattc ctcggtatgg aaccgctgcc
      781 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
      841 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
      901 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
      961 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
     1021 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
     1081 cgtgatgatt gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca
     1141 catgaaggcg tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgcatcct
     1201 gcaacctggg atttaaacct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
     1261 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
     1321 cgagcgtttc ttcgccagca gcatatccat catcttgtca gtacgctgat cgtcttcatc
     1381 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg
     1441 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
     1501 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
     1561 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
     1621 cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga
     1681 gtccgcatct gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc
     1741 tggatcgata ccgatcgcta ccgaaatatc gtgcacttcc tgcgaagcca gcacttcgtc
     1801 ggaagagact tcccaggtgt taaggatctt acctttcagt ggcatgatcg cctgatattc
     1861 gcgatcgcgc gcctgcttgg cagatccgcc tgcagagtca ccttccacaa ggaacagttc
     1921 ggtacggtta agatcctgcg cggtacagtc agccagtttg ccaggcagcg ccgggccgct
     1981 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
     2041 cgccatctcc gccagcagct ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc
     2101 atctttcacc acgcctgaaa cgaatgccgc acactgacgc gaagagagac gctctttggt
     2161 ctgcccggca aactgcggat cctgcatttt caccgacaga acataggcgc agcgatccca
     2221 gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc
     2281 acgcatcgcg tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
     2341 tgggataagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
     2401 cgcccagtcc acagcttcag tatcaccagc gaaattaccg ataaacggtt tttccggcag
     2461 cgtcggcaga ccatttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
     2521 gcgttgttcg gtattgttga tctcatcttt aaaagtgatc tcaacgccag ggcacaatac
     2581 cgctttggct ttcagcacat gtgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa
     2641 ggtttcatcc ggccagaagt gcacgctggt accagtattg cgtttaccgc aagtgccgac
     2701 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
     2761 gcggcgcacg ttaacttcta cgcgcttcga caaggcgtta actaccgaaa tccccacgcc
     2821 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcccg cgtgcagacg
     2881 gcaaagaatc agttctaccg ccggtacccc ctcttccggg tgaatatcca ccggcatccc
     2941 gcgcccatcg tcaatcactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt
     3001 tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt
     3061 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
     3121 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
     3181 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
     3241 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
     3301 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
     3361 gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca
     3421 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa
     3481 acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgt gacaaccagg
     3541 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
     3601 ggacaatgga ttccagcagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga
     3661 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
     3721 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
     3781 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
     3841 atcagcatgt aactcgagag tacgccagcc gggcgcgatg gtgtccagcg taaagttgga
     3901 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
     3961 gtcgagatcc tgctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
     4021 cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca
     4081 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
     4141 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
     4201 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg
     4261 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgccagg
     4321 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
     4381 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctgatg
     4441 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
     4501 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc
     4561 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
     4621 ataaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
     4681 ctgattaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
     4741 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
     4801 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
     4861 tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
     4921 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
     4981 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
     5041 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
     5101 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
     5161 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
     5221 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
     5281 gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg
     5341 acgggcaaca tcttccacac tttcaccctc ttctatcatc ccggcaacca tctccagtag
     5401 ccagggggtt tcactggtgt cgtatgcggc aatccgaatc tgctcaatca gcacaacttc
     5461 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttccct
     5521 ccgtacctca tgactcattt acccgttgaa taaacgatga cgaaatctat aaagatctaa
     5581 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
     5641 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta
     5701 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac
     5761 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
     5821 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
     5881 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
     5941 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
     6001 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
     6061 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
     6121 aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
     6181 tggcgtgcgt taacgctgca ggaaaaagct gcagggattc aggacgtcac atatcagacc
     6241 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
     6301 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
     6361 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
     6421 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
     6481 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
     6541 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
     6601 ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg
     6661 caggatggtc acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct
     6721 tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc
     6781 cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
     6841 caggtgaaac aggcacagta caactttgtt ggtgccagcg agcaactgga aagcgcacat
     6901 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagc
     6961 attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg
     7021 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac
     7081 aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt
     7141 aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc
     7201 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
     7261 gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact
     7321 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
     7381 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
     7441 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
     7501 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
     7561 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag
     7621 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
     7681 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
     7741 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcaa
     7801 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg
     7861 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
     7921 ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagtccggc ttacggtaaa
     7981 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
     8041 ccgaagacag ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
     8101 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caactggcac ctcttctcgt
     8161 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
     8221 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
     8281 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
     8341 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
     8401 ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc
     8461 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaactgc
     8521 tggaaaataa cgccgataca ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
     8581 ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc
     8641 tgcaagaaaa gctaatcgat cgctttgtcg agttgcgtga acagtatggc ttccagttgc
     8701 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
     8761 actgcgcgac ggaagctgaa atcgctaccg agttcctcta cattgacgat atcgggttag
     8821 gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt
     8881 atccatggga atttatgttg cgtgagatgt tttccaccaa gctggaagat gctggcgtac
     8941 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
     9001 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
     9061 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
     9121 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
     9181 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgttg attggtagct
     9241 ggctggtgaa cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc
     9301 aggatatgtc tcggttttat ccacatattt ttgttgagta atcaatgatg ccggatggca
     9361 ttcgccatcc ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc
     9421 tataccatcg accggaatgg taattggctc ctgccaatcc cacgcaccta agacatacaa
     9481 caacggcaga tagtgctctg gcgttggggt cgataacgca ccaccttcat ggtcgaggta
     9541 attcaccaga ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc
     9601 attaaacgac atcgcccaag gatacggtga actatcaccg tgccacttca ctgtacgcag
     9661 gttatgcacc acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag
     9721 tttgcgcccc atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac
     9781 catcgggata tcggcgtcag gatacatctt aatcagcaca ccccaggaac cgtggtcaaa
     9841 gccccaggct tctttatcca gcgtcaccgg gaccggcgct aacagctcaa ccagatgctg
     9901 tgccagcaca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
     9961 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    10021 ccaatgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
    10081 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    10141 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    10201 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    10261 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    10321 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    10381 cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat
    10441 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    10501 ggtgttgggt tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat
    10561 gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc
    10621 gatcaagcgc acagccattc tgcttactct cggcatcagt ctgcataact tcccggaagg
    10681 gattgccacc tttgtcacgg cgagtagcaa cctggagctg ggatttggca tcgctctggc
    10741 cgtagcattg cacaatattc ctgaaggtct ggcggttgca ggcccggttt acgcagcaac
    10801 ggggtctaaa cgcacggcga ttctgtgggc ggggatttct ggactggcag aaattcttgg
    10861 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat
    10921 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc
    10981 caaagagatt gaccccaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    11041 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaagcaac atcagaagtt
    11101 gtccagcgca tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg
    11161 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    11221 ggcgaaacgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa
    11281 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    11341 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    11401 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    11461 ttctcataga aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa
    11521 gccagctaca acttcggttt cttgattaag gcagtaaatt aagcagcggt tttcagctgg
    11581 ctttacgctc atgtgcctga cggtatgcca ccaggtcttc aatagttacg agcgccatat
    11641 tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag
    11701 tcagctcaca cagcacacca gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg
    11761 cttcagtatg accgccacgc gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt
    11821 ggcccgggcg attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg
    11881 taatacgatc agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg
    11941 taaaaccggt gccataggcg ctggtgttat tttctaccat cattggcaga tcaagttgtt
    12001 tacggcgatc ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg
    12061 ccatctgctc aacggtcatg gtttctgccg ggaagatcat atcgccttcg ttttcacggt
    12121 cttcatcatc aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa
    12181 cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc
    12241 actaaaatta tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat
    12301 aacgtgagcg ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt
    12361 cggccccgga attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg
    12421 ctttcaactg agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg
    12481 attcactata acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta
    12541 tgacttaccc ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa
    12601 tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag
    12661 gaatcaggga gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagctcagc
    12721 tgacgcgcct cgaccttgta agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac
    12781 ggactcgtga gaaactggcg ctgcttgaac aaagaatgag cgaactggaa aatcgcagta
    12841 ctgagattaa aaagcagcca gatccagaga ctctcccccc aacgctgtaa acatttgtct
    12901 ttaatgcact gtaaattatg aatt
//
LOCUS       DAZFAH010000134        12905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFAH010000134 DAZFAH010000000
VERSION     DAZFAH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.134"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..1765)
                     /gene="pgi"
                     /locus_tag="ACHJWB_003416"
     CDS             complement(116..1765)
                     /gene="pgi"
                     /locus_tag="ACHJWB_003416"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4314990.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFVAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2002..2198
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2290..3639
                     /gene="lysC"
                     /locus_tag="ACHJWB_003417"
     CDS             2290..3639
                     /gene="lysC"
                     /locus_tag="ACHJWB_003417"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4314991.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3892..4164
                     /locus_tag="ACHJWB_003418"
     CDS             3892..4164
                     /locus_tag="ACHJWB_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4314992.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="ACHJWB_003419"
     CDS             complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="ACHJWB_003419"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4314993.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRYTSPGRKKKGR"
     gene            5381..6070
                     /gene="pepE"
                     /locus_tag="ACHJWB_003420"
     CDS             5381..6070
                     /gene="pepE"
                     /locus_tag="ACHJWB_003420"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4314994.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6161..7792)
                     /locus_tag="ACHJWB_003421"
     CDS             complement(6161..7792)
                     /locus_tag="ACHJWB_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4314995.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELHKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(8012..11695)
                     /gene="metH"
                     /locus_tag="ACHJWB_003422"
     CDS             complement(8012..11695)
                     /gene="metH"
                     /locus_tag="ACHJWB_003422"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4314996.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            11895..12719
                     /gene="iclR"
                     /locus_tag="ACHJWB_003423"
     CDS             11895..12719
                     /gene="iclR"
                     /locus_tag="ACHJWB_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4314997.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLRAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2968 a         3464 c         3341 g         3132 t
ORIGIN      
        1 aaaatctatt ttaggattaa cctcacggta tgatttccgt taaatcacag acaaggggat
       61 ttatctgata aaacaatgcc gacataacgt cggcattgtt attaaggttt ttaaattaac
      121 cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg ctgctgattt
      181 ctttatcatc tttcagctct ggcagaatac ggtttgccag ttgtttaccc agctccacgc
      241 cccactggtc gaaggtgaag atgttcagga tcacgccctg agtaaagatt ttgtgctcat
      301 acagcgcaat caacgcaccc aggctgaacg gagtgatttc acgcagcagg atggagttgg
      361 tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt gccggatctt
      421 taccctgatc gcgatattcc tgctcaacca cttcgcggga tttaccaaac gccagcgctt
      481 cagtctgagc aaagaagtta gacagcagtt tctggtgatg atcagagagc gggttatggg
      541 tgatagccgg agcgacgaaa tcgcacggta ccattttggt tccctggtgg atcagctggt
      601 agaacgcgtg ctgaccgtta gtgcctggtt caccccagat aatcgggcca gtctggtaat
      661 ccacaacgtt accgttacgg tcaacatact taccgttgga ctccatattg ccctgctgga
      721 agtacgccgc gaaacggtgc atatactggt catacggcag aatcgcctca gtttccgcac
      781 caaagaaatt gttgtaccag atgccaatca gcgccagcag tacaggcagg tttttctcgg
      841 caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc agaaagcagt tcaacgaagt
      901 tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa gagtaacgac
      961 cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac tcgccaacgg
     1021 ctttggcatt ggttgagagc gccgcaaagt gtttcgcaac gtgtttttca tcacctgccg
     1081 ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat ggtttcctga gtggtgaagg
     1141 ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc acttcagcga
     1201 tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtgatttttg tacggacgca
     1261 gtgcttcggt caccatgtac gggccgaggt cagaaccgcc gatcccgatg ttcactacgt
     1321 cagtgattgc tttgccggta taacccttcc actcaccgga gataatcgct tccgagaagg
     1381 ttttcatctt ctccagcacc gcgttgactt ccggcattac gtctttgcca tcaaccaaaa
     1441 tcggggtatt gctacggtta cgcagcgcta cgtgcagcac agcgcggttt tcagtgcggt
     1501 tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac tctttcgcca
     1561 gatcttgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa tccaccagca
     1621 tctgatcgcc gaaggttgcg gagaacttag aaaaacgatc gccgtcttta gcaaaaagat
     1681 cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc caggcagcgg
     1741 tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga attgtgactt
     1801 tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt tagcgtaatg
     1861 ttgagtgtaa acccttagcg cagtgaagca tttattagct gaactactga ccgccaggag
     1921 tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc ccgctcaccc tttatttata
     1981 aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta acggtgttgg
     2041 aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt gaacggctgg
     2101 ccacgttcat catcggctac aggggctgaa tcccctgggt tgtcaccaga agcgttcgca
     2161 gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga agtatcgctc
     2221 tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat cccctgacac
     2281 gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt agctgatttt
     2341 gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtgcg tttagtagtc
     2401 ctctcggctt ctgctggtat cactaatctg ctggtcgctt tagcggaagg gctggaacct
     2461 ggcgagcgat tcgaaaaact cgacgctatt cgcaacatcc agtttgccat tctggaacgt
     2521 ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tgctggagaa cattactgtt
     2581 ctggcagaag cggcggcgct ggcaacgtct ccggcgctga cagatgagct ggtcagccac
     2641 ggcgagctga tgtcgaccct gctgtttgtc gaaatcctgc gcgaacgcga tgttcaggca
     2701 cagtggtttg atgtacgtaa agtgatgcgt accaacgacc gatttggtcg tgcagagcca
     2761 gatgtagccg cgctggcgga actggccgcg ctgcagctgc tcccacgcct caatgaaggc
     2821 ttagtgatca cccagggatt tatcggtagc gaaaataaag gtcgtacaac gacgcttggc
     2881 cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc atctcgtgtt
     2941 gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtagt ttccgcagca
     3001 aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt tggtgcaaaa
     3061 gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccggt ctttgtcggc
     3121 tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga aaatccgccg
     3181 ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc ttactttgca cagcctgaat
     3241 atgctgcatt ctcgcggttt cctcgcggaa gttttcggca tcctcgcgcg gcataatatt
     3301 tcggtagact taatcaccac gtcagaagtg agcgtggcat taacccttga taccaccggt
     3361 tcaacctcca ctggcgatac gttgctgacg caatctctgc tgatggagct ttccgcactg
     3421 tgccgggtgg aggtggaaga aggtctggcg ctggtcgcgt tgattggcaa tgacctgtca
     3481 aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa cattcgcatg
     3541 atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga agatgccgag
     3601 caggtggtgc aaaaactgca tagtaatttg tttgagtaaa tactgtatgg cctggaagct
     3661 atatttcggg ccgtattgat tttcttgtca ctatgctcat caataaacga gcctgtactc
     3721 tgttaaccaa cgtctttatc ggagaataat tgcctttaat ttttttatct gcatctctaa
     3781 ttaattatcg aaagagataa atagttaaga gaaggcaaaa tgaatattat cagttctgct
     3841 cgcaaacgaa ttcccgtaca ctgaactctc tggctcactg agggcaccat catggcactc
     3901 ccccgtatta cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa aacactgctg
     3961 gatcgggcgc ggattgcgca tggtcgcgta ctgaccaact ccgaaaccaa cagcattaag
     4021 aaagagtaca tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa agcccgccaa
     4081 ttgaagaaaa agcaggctta taaaccggat cctgaagcgt catttagctg gtcagcaaat
     4141 acgtcgacgc gcggcaggcg ctaatgaaca atcaacgcct gagattttca ctcgtctgtg
     4201 tggacacaat cttcccctca ccctaaccct ctccctgaag gggcgagggg actgtccgag
     4261 ctctttttgg acgttttcat cagtctgagg cgttaatcag cgtcccttct ttttacgccc
     4321 cggcgaggta taacgcttac cgttggacgc cgggcgaccg cctttttccg ccgttttttc
     4381 catcttaacg actggacgtt tgatgcccgc tgttttcggt ttggccttcg ctttaggttt
     4441 tgcctcggaa gaggaatttt cgatgagctt aaagaggtcg attaactcat cgtcggttaa
     4501 atcgcgccac tcccccagcg gaatgccgct taagctgacg ttcataatgc gcgtacgttc
     4561 cagctttttc acttcatagc cgaaatgctc gcacatgcgc cggatctgac ggttcagccc
     4621 ctgcaccagg gtaatgcgga agacaaacgg cgcttctttt ttgactttgc actttttcgt
     4681 caccgtcccg aggatcggca cccccgcccc cataccacga ataaattcat cggtaatcgg
     4741 tttatcgacc gtcaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt
     4801 caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gagtctttat ccaggcggcc
     4861 aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctcgccatc
     4921 ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcggc
     4981 ttcccgaggc tcaatcaact gaccgtttac tttcacaacg tcgccgggtt tcacctgatc
     5041 gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc
     5101 ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc
     5161 gggcagcata aattctcctg taaaaaacgg aatataccgt acctttgggt tgataaaaaa
     5221 tagtcatgag cgaactattt gttatttcca ttggcttgaa ttcgttacct gctcaaatca
     5281 ctttccagaa gcaatgtgcg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg
     5341 gtagcatgcc tcccgtgatt tcttactaac tggagaagta atggaactgc ttttattgag
     5401 taactcgacg ctgccgggta aagcctggct ggaacatgcg ctgccgctga ttgctgaaca
     5461 gttgcagggt cgccgttcag cggtatttat tcctttcgct ggcgtaacgc agacctggga
     5521 tgattacaca gcgaaaacgg ctgcggttct cgctccgcta ggtgtttctg tcaccggtat
     5581 tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgct gagatcgtga ttgtcggcgg
     5641 cgggaatact ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg caccaattac
     5701 tgacgtggtt aaacgtggtg cgctgtatat tggctggagc gcaggcgcta accttgcttg
     5761 cccaaccatt cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct
     5821 aaatctgttc ccgctgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa
     5881 aggtgaaacc cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat
     5941 tattggtcta ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg
     6001 cccgaacacc acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca
     6061 ccgtttttaa gtgaattgcc ggatgcatga tccggcatct ttttcatagt gcctgatgcg
     6121 acgcttgacg cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt
     6181 catcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc
     6241 gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt
     6301 gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat
     6361 gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg
     6421 ccagctttaa attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa
     6481 gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga
     6541 cgatatcgga ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg
     6601 actcttcctc cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt
     6661 agagaacgtt gatatcatct gccagcttat gcagctcttt ctcctgccgt ggctcgccgt
     6721 gcatcacttt atttagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt
     6781 cgcgcgcggc gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt
     6841 tgggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg
     6901 ccatcgggtc aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga
     6961 aatagatcac cagttccgct tttggcaatg acaacttccc cattgtctct gccagcaaat
     7021 ggacaaacgg caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga
     7081 cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcaga ccggaaccga
     7141 ggttagcacc aatcaccaga cagagcgcca cggggaagga gataatgcct gcggcggtca
     7201 gagtcgcggt cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc
     7261 caatcagcgc atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt
     7321 ttgcctgggt gatcggcgtt acggcctgca caatcaactc cagcgctagc aaaatcagcc
     7381 caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa
     7441 aaatcacgcc gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac
     7501 gcgccattag cgccgtcccg acatctgcac ccagcacaat gaccagagcc ggtgcgaggg
     7561 ctaccagatc ctgagcgaca aacgaggtca ccagcatggt ggtggcatta ctgctctgta
     7621 ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc
     7681 taaggacagt acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag
     7741 tcccccagac cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag
     7801 gttctcctta tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc
     7861 tgactggcat ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc
     7921 tgtttccagt ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt
     7981 ttgttgtaag gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg
     8041 ccagccagcg ctcaacttcg gtaacgctca tacctttacg gcgggcataa tcttcaacct
     8101 gatcgcgctg aatttgtgct acagcgtagt acttgctgtc cgggtggctg aagtaccaac
     8161 ccgaaaccga tgcaccgggc cacatggcga aagattctgt gagtttcatg ccagtgtgtt
     8221 tttccacttc cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat
     8281 agcccggtgc cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt
     8341 tctcgttcgg cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa
     8401 aggcttccgc cagacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct
     8461 gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc
     8521 cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag
     8581 cgaatcccgt tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc
     8641 gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca
     8701 ccacgccacg cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt
     8761 ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact
     8821 tcccggccag cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagagttt
     8881 cgatgctggc ttcaacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct
     8941 gccagtcaaa agcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgcg
     9001 gtttcttacg cccgtgctga atacgtacgg tttcgtactc cttgcgggta cgagcgacaa
     9061 aatcatcacg ctgagtatcg gaaagcagcg ccgccaccac accaacggtg cgcgatgcgt
     9121 tctgtacata caccgtcggg ccgctgtagt tctgctcaat tttcaccgcc gtgtgcgctt
     9181 ttgaggtcgt cgcgccgcca atcaatagcg ggatggtgaa gccctgacgc tccatctctt
     9241 tcgccacgtt aaccatctcg tccagcgacg gcgtgataag ccccgaaagt ccaatcagat
     9301 cagcattcac ttctttagcg gtacggagaa ttttttccgc aggcaccata acgccgagat
     9361 cgacaatttc gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt
     9421 ggacgtcgcc cttcacggtg gcgatcacca tcttgccgtt ggttttgccc tgctctttgc
     9481 tggcttcaat aaacggttcg agatacgcca ccgcctgttt catgacgcgc gccgatttga
     9541 ccacctgtgg caggaacatt ttcccttcgc caaacagatc gccgaccacg ttcatgccgt
     9601 ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcgg
     9661 tatcctgctc gataaactcg gtaatgcctt tgaccagcga gtattccaga cgtttattca
     9721 cttcccacga gcgccactcc gcctgctggg cgttagcggt gtcgtcggtt ttgctgccgc
     9781 gatatttctc ggcaagctcc agtaaacgct cagtgccatc atcgcgacga ttaagaatca
     9841 catcttccac cgcgtcgcgc agttcagcgg gcaggtcgtc gtaaatcgcc agctgcccgg
     9901 cattgacgat ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa
     9961 tggcttcgcg caccggatcg ttgccacgga acgagaaaga aacgttagat acgccgccgg
    10021 aaattagcgc gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg
    10081 cgtagttgtt gtgctcttca atgccggtgg caaccgcgaa gatgttcggg tcgaagatga
    10141 tatcttctgg cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct
    10201 cgattttccg tgcgcgggta tcggcctgtc cctgttcgtc aaaggccatt accaccactg
    10261 ccgcaccgta acgacgcaag agcttcgcgt gatggataaa ggcgtcgacg ccctctttca
    10321 tcgagataga gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat
    10381 cccattttga ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca
    10441 gattgagaaa acgcaccatc gccgcttcgg cgtcgagcat cccttcatcc atgttgatat
    10501 cgataatctg cgcaccgttt tccacctgct ggcgggccac atccagtgcc tcgctgtatt
    10561 tctcttcttt gatcaggcgc ttgaacttag cggaaccggt gacgttggtg cgttcaccca
    10621 cgttcacaaa caggctatct tcgccaatgt tcagcggctc caggccggac aaacggcagg
    10681 ctacggggat ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg
    10741 caatatgttg tggcgtggtg ccacagcagc cgccgacgat attgagaaaa cccgcttgcg
    10801 cccattcacg tatctgtttt gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt
    10861 tgggtagccc ggcgttcggg tgcgcggtga cgtagcattc cgcaatccgt gacagctcct
    10921 gcacgtactg gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagagctt
    10981 cggcgtggcg caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg
    11041 aggcgtcggt gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact
    11101 ccgttttcac cgcaaaaacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca
    11161 ggatcagatc cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccacca
    11221 gctggtcaaa agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg
    11281 tgcggttggt tgggccgaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg
    11341 tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca
    11401 gggattccat ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga
    11461 tgatatccgc gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttgc
    11521 tgagcaccag caggtcgttg ttgcctttga gatcgcatgg ccagtcggca aagcgttcac
    11581 cacgaaaatc ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca
    11641 gcaccagaat acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg
    11701 ctcccgacac gctcaaccca gatttaataa aaattcaaca aaccatactg gcataaacgc
    11761 atctgtggta aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat
    11821 cagttcagca actattgcat tagctaacaa taaaaatgaa aatgatttcc acgatacaga
    11881 aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg
    11941 ccaccgcacc agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt
    12001 ggattgccga atccaatggc agtgtggcac tcacagagct ggcgcaacaa gccgggttac
    12061 ccaattccac gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg
    12121 tcggcgaact gggacattgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc
    12181 tccagagccg taatttgtta gcgattgttc accctatcct gcgcaattta atggaagagt
    12241 ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg
    12301 accaggtaca gtgtacgcat ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga
    12361 tgcacgcttc cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga
    12421 agctgctgca ccgcaaaggg ttacgtgcct atacccacgc aacgctggtg tctcctgtgc
    12481 atttaaaaga agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac
    12541 atgcactggg gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg
    12601 ccgcaatttc tatttccgga ccgatttcac gtattaccga tgaccgcgtg accgagtttg
    12661 gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac
    12721 tttttctggc gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg
    12781 gtgtctgtta atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta
    12841 ttaactcagc attattttta aacatcaaac cacttaatta ttaacaaaat cgcaagatct
    12901 cattc
//
LOCUS       DAZFAH010000135        12874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFAH010000135 DAZFAH010000000
VERSION     DAZFAH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.135"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            190..1704
                     /gene="caiT"
                     /locus_tag="ACHJWB_003424"
     CDS             190..1704
                     /gene="caiT"
                     /locus_tag="ACHJWB_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4314998.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1735..2877
                     /gene="caiA"
                     /locus_tag="ACHJWB_003425"
     CDS             1735..2877
                     /gene="caiA"
                     /locus_tag="ACHJWB_003425"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4314999.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3006..4223
                     /gene="caiB"
                     /locus_tag="ACHJWB_003426"
     CDS             3006..4223
                     /gene="caiB"
                     /locus_tag="ACHJWB_003426"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4315000.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKRVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4297..5850
                     /gene="caiC"
                     /locus_tag="ACHJWB_003427"
     CDS             4297..5850
                     /gene="caiC"
                     /locus_tag="ACHJWB_003427"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4315001.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKSGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5959..6744
                     /gene="caiD"
                     /locus_tag="ACHJWB_003428"
     CDS             5959..6744
                     /gene="caiD"
                     /locus_tag="ACHJWB_003428"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4315002.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6750..7340
                     /gene="caiE"
                     /locus_tag="ACHJWB_003429"
     CDS             6750..7340
                     /gene="caiE"
                     /locus_tag="ACHJWB_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4315003.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7426..7821)
                     /gene="caiF"
                     /locus_tag="ACHJWB_003430"
     CDS             complement(7426..7821)
                     /gene="caiF"
                     /locus_tag="ACHJWB_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4315004.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8082..11303)
                     /gene="carB"
                     /locus_tag="ACHJWB_003431"
     CDS             complement(8082..11303)
                     /gene="carB"
                     /locus_tag="ACHJWB_003431"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4315005.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVSLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11321..12469)
                     /gene="carA"
                     /locus_tag="ACHJWB_003432"
     CDS             complement(11321..12469)
                     /gene="carA"
                     /locus_tag="ACHJWB_003432"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4315006.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3061 a         3332 c         3384 g         3097 t
ORIGIN      
        1 gagaaaagcg ccctctattc acccgaaaca aaaatgtgat aacaatcaca gaatgcagct
       61 tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata ataattatat
      121 taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt ttcacagtgg
      181 aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt tttctttccg
      241 ccgttaataa tcgtcggcat actttgttgg cttacagtca gagatctgga tgcagcgaat
      301 gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc atttgaatgg
      361 tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta tgccaaaaag
      421 cgtttaggta acgaaccacc tgaatttagt accgccagtt ggatctttat gatgttcgcc
      481 tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta ctacatctcc
      541 accccgccgt ttggcttaga accgaactcg acaggggcga aagagttggg gctggcttac
      601 agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc agtcgccttc
      661 gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac actggtgccg
      721 ctggtaggtg aaaaacacgc caaagggttg ttcggcacta tcgtcgacaa cttctatctc
      781 gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct ggtgaccgag
      841 tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat catcattacc
      901 tgctggatta tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc
      961 gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt cattgtcagc
     1021 ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct gatgtatctg
     1081 ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca gggctggacc
     1141 gtgttctact gggcatggtg ggtgatttat gctatccaga tgagtatctt cctcgcccgc
     1201 atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgctggg gctgacagcg
     1261 tcaacctgga tcctgtggac tgtactcggt agtaacactc tgctgttgat agataaaaac
     1321 atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat cattgaaacc
     1381 tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct ctgctttatt
     1441 gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtccac ttgccgcgaa
     1501 gtacgcgatg gtgaagaacc acctctgctg gtgcgtatcg gttggtcaat tctggttggc
     1561 attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac cgccattatc
     1621 gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt tattaaagac
     1681 gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg aaagatggat
     1741 tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact gatggccagc
     1801 gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt
     1861 gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga gcacggtggt
     1921 ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg tctgggggca
     1981 ccaacctatg tactgtacca gttgccgggc gggttcaaca ccttcctgcg cgaaggcaca
     2041 caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat gtggaactca
     2101 gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac ttatacccgt
     2161 agaaatggta agatttatct taatggtagt aagtgtttta ttaccagcag cgcctacacc
     2221 ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa
     2281 tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgaaaa gctcggtctg
     2341 cgtatggata gctgctgtga aatcaccttt gacgacgtgg aactggacga gaaagacatg
     2401 ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc
     2461 ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac
     2521 gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat tcaggaaaaa
     2581 ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga agcagcgtgg
     2641 aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata cttctgcgcc
     2701 aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg
     2761 ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt ctccggggga
     2821 tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc
     2881 aatgcgctgc ctgatgcgac gctgatgcgt cttatcaggc ctacgggagc acgaatgtag
     2941 gccggataaa gcgtttacgc cgcatccggc agtcatgcgc tacataaatt tttcaggagc
     3001 aaataatgga tcatctaccc atgccgaaat tcgggccgtt ggccggattg cgcgttgtct
     3061 tctccggtat cgaaatcgcc ggaccgtttg ccgggcaaat gttcgcagaa tggggcgcgg
     3121 aagttatctg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc
     3181 cgcaactctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc
     3241 gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc agtaaaggtc
     3301 cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac ccgaaacggg
     3361 ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc aatcttccgg
     3421 cctataacac tatcgcccag gcctttagtg gttacctgat tcagaacggt gatgttgacc
     3481 agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc gccaccacgg
     3541 cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt atcgacatcg
     3601 ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac ttcaacggcg
     3661 gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt tgcggtctgt
     3721 ataaatgcgc cgacggctac atcgtgatgg aactggtggg cattacccaa attgaagagt
     3781 gctttaaaga tattggcctc gcacatctac tcggtacgcc ggaaatcccg gaaggcactc
     3841 agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa ctcgatgcct
     3901 ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg aatatcgcct
     3961 gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat
     4021 caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac atcatgccga
     4081 aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc atggacacgg
     4141 ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg gtcagcaaag
     4201 gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg
     4261 cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg cggacaacat
     4321 ctacgtcaaa tgtgggacga tcttgcggac gtttacggtc ataaaacggc gctgatttgt
     4381 gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac
     4441 cgcacggcaa acctgtttta tacgctaggg attcgcaaag gcgacaaggt tgctctacat
     4501 ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt
     4561 atggtgccga ttaacgcccg cctgttacgc gaggaaagcg cgtggatcct gcaaaatagc
     4621 caggcgtgcc tgctggtgac cagtgcgcaa ttctatccta tgtatcaaca gattcagcag
     4681 gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc cgctgatgat
     4741 ggcgtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg ctatgcaccg
     4801 ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg
     4861 aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc cgcctggcag
     4921 tgtgcactgc gtgacgatga cgtctacctg acggtaatgc ctgcgtttca tatcgattgc
     4981 cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa
     5041 tacagcgccc gcgccttctg gggacaggta cagaagtacc gcgccaccgt taccgaatgt
     5101 attccgatga tgatccgtac cttgatggtg cagccgcctt cagcgaacga tcggcaacac
     5161 cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga tgcgttttgt
     5221 gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgttggcatt
     5281 atcggcgatc gccctggcga taaacgacgc tggccgtcga ttggtcgggc ggggttttgc
     5341 tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa
     5401 atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa
     5461 gccactgcga aagtgctgga agccgacggc tggctacata ccggcgatac cggatactgc
     5521 gacgaagagg gcttttttta tttcgtcgat cgccgctgca atatgatcaa aagcggtggc
     5581 gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccaaa aattcaggat
     5641 atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg
     5701 ctgaatgaag gtgaaacatt gagcgaagag gaatttttcc gcttctgcga acaaaatatg
     5761 gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg
     5821 gggaaaataa ttagaaagaa tctgaaataa atgaaacagc agggaactac cctgccagct
     5881 aataatcacg ctataaaaca atatgcattt tttgcaggga tgttgtcgtc cctgaaaaag
     5941 caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg
     6001 gaaattaccc ttgatcgtcc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg
     6061 ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt
     6121 gccggagaga agttcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca
     6181 ccggatgctg actttggccc gggtggattt gcgggattaa ccgaaatttt caatctcgac
     6241 aaaccggtta tcgcagctgt gaacggctat gcctttggcg gcggtttcga actggcgctg
     6301 gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga agccaagctg
     6361 ggcatcgttc ctgacagcgg cggcgtgctg cgtctaccga agatcctgcc gcctgccatc
     6421 gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct gcgttggggg
     6481 atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggcgcag
     6541 cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatctt ccgcaccacc
     6601 agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat
     6661 ccgtcggttc tgcattcgga ggatgccatt gaagggccgc tggcgtttgc cgagaagcgc
     6721 gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag gggttaattc
     6781 cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc gatgtgattg
     6841 tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac gggcggttga
     6901 tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg
     6961 acaccatcgt tggggaaaac ggccatatcg ggcacggagc gatcctgcat ggttgtgtga
     7021 ttggtcgcga tgcattggtc gggatgaaca gcgtgattat ggatggcgcg gtcattggcg
     7081 aagagagcat tgttgccgcc atgagctttg tcaaagcagg ctttcacggc gagaaacgcc
     7141 agttgttgat gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac gagttacact
     7201 ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac
     7261 atgaaacaca gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg
     7321 atgtgacgcc taaacggtga tcttgccgga tgcgacctgg ttgctggata agatgctaca
     7381 acatcgcatc tggcaagcag gacgcccggt atcaacgaga taaaattaac gacgcatact
     7441 ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg gaacggcagg
     7501 aatacgcggc gtctttctta cgccgactaa tttatcccgg ctgttattgc gcaggcgcgc
     7561 gtaaatttgc tcatcgatat cgaccacttt taccagtcgc tggcactggc atccccgtcc
     7621 ttccagctta ttagggatca tcttaacttc gcagcttatt tctgcgactt ccgacagaat
     7681 ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag agatctctct
     7741 tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt agagtggttt
     7801 ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc tggctatgac
     7861 tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga tagtatgcaa
     7921 cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata tcgattcccc
     7981 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
     8041 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
     8101 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
     8161 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
     8221 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
     8281 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
     8341 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
     8401 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
     8461 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
     8521 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
     8581 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
     8641 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
     8701 acacgcgccg ccacttttgc cagcgatacg ccggtggctt tggagacgaa cggaacggta
     8761 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
     8821 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
     8881 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
     8941 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     9001 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     9061 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     9121 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     9181 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     9241 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     9301 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     9361 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     9421 tctagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     9481 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     9541 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     9601 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     9661 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     9721 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     9781 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     9841 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     9901 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     9961 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
    10021 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
    10081 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    10141 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
    10201 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    10261 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
    10321 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    10381 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    10441 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatt
    10501 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
    10561 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    10621 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    10681 tcatcaatca gcagctcctt ggttggggag agatccagac cgcgggcgca aatttcttca
    10741 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    10801 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
    10861 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    10921 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
    10981 aacacgccct gacgctccag ctccagcgcg cagttcagcg ccgtctgacc gcccatcgtt
    11041 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
    11101 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    11161 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    11221 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    11281 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    11341 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
    11401 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
    11461 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    11521 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    11581 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    11641 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
    11701 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    11761 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    11821 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    11881 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    11941 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    12001 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
    12061 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    12121 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    12181 cttcggtatt acggaagttg ctggcaatca gcggcagatc gcgaatcacc agaccttgtg
    12241 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    12301 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    12361 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    12421 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
    12481 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
    12541 atctgcttac cgacgaattt ctggcaaacg gcagcattct ggagatatgg gcgctaaaag
    12601 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
    12661 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcgcagcaa gaagattgca
    12721 tgaaataaaa gcgagcaggg aaaagtggac caaatggtca aaatttacat taaaacaaca
    12781 aaaaccgaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
    12841 aaaaataaaa gggccaaaaa ttaaagccct ttac
//
LOCUS       DAZFAH010000136        12847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFAH010000136 DAZFAH010000000
VERSION     DAZFAH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.136"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..116
                     /locus_tag="ACHJWB_003433"
     CDS             <1..116
                     /locus_tag="ACHJWB_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HFO4315007.1"
                     /translation="ACTGHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ"
     gene            411..1748
                     /gene="frlA"
                     /locus_tag="ACHJWB_003434"
     CDS             411..1748
                     /gene="frlA"
                     /locus_tag="ACHJWB_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4315008.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1769..2791
                     /gene="frlB"
                     /locus_tag="ACHJWB_003435"
     CDS             1769..2791
                     /gene="frlB"
                     /locus_tag="ACHJWB_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4315009.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2841..3671
                     /gene="frlC"
                     /locus_tag="ACHJWB_003436"
     CDS             2841..3671
                     /gene="frlC"
                     /locus_tag="ACHJWB_003436"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4315010.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFASDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3668..4453
                     /gene="frlD"
                     /locus_tag="ACHJWB_003437"
     CDS             3668..4453
                     /gene="frlD"
                     /locus_tag="ACHJWB_003437"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO4315011.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQTPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4553..5284
                     /gene="frlR"
                     /locus_tag="ACHJWB_003438"
                     /gene_synonym="yhfR"
     CDS             4553..5284
                     /gene="frlR"
                     /locus_tag="ACHJWB_003438"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4315012.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGISQGKATK
                     EKVIEQERVSAAPFCEKLNISGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5436..6521)
                     /gene="yhfS"
                     /locus_tag="ACHJWB_003439"
     CDS             complement(5436..6521)
                     /gene="yhfS"
                     /locus_tag="ACHJWB_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4315013.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDIICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYGLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6533..7837)
                     /gene="yhfT"
                     /locus_tag="ACHJWB_003440"
     CDS             complement(6533..7837)
                     /gene="yhfT"
                     /locus_tag="ACHJWB_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO4315014.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGVVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7849..8202)
                     /gene="yhfU"
                     /locus_tag="ACHJWB_003441"
     CDS             complement(7849..8202)
                     /gene="yhfU"
                     /locus_tag="ACHJWB_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO4315015.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8213..9091)
                     /gene="php"
                     /locus_tag="ACHJWB_003442"
     CDS             complement(8213..9091)
                     /gene="php"
                     /locus_tag="ACHJWB_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO4315016.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9088..10314)
                     /gene="yhfW"
                     /locus_tag="ACHJWB_003443"
     CDS             complement(9088..10314)
                     /gene="yhfW"
                     /locus_tag="ACHJWB_003443"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4315017.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWGNQSVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQIGRVITFGGLLTDSQRILDAAESKEGCFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10315..11478)
                     /gene="yhfX"
                     /locus_tag="ACHJWB_003444"
     CDS             complement(10315..11478)
                     /gene="yhfX"
                     /locus_tag="ACHJWB_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO4315018.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(11562..11924)
                     /gene="yhfY"
                     /locus_tag="ACHJWB_003445"
     CDS             complement(11562..11924)
                     /gene="yhfY"
                     /locus_tag="ACHJWB_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO4315019.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11941..12846)
                     /gene="yhfZ"
                     /locus_tag="ACHJWB_003446"
     CDS             complement(11941..12846)
                     /gene="yhfZ"
                     /locus_tag="ACHJWB_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO4315020.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
BASE COUNT         3020 a         3649 c         3216 g         2962 t
ORIGIN      
        1 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
       61 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
      121 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
      181 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
      241 tttgataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
      301 cacattacat aacatcatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
      361 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
      421 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
      481 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
      541 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
      601 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
      661 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
      721 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
      781 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
      841 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
      901 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccccta
      961 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
     1021 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
     1081 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
     1141 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
     1201 cgccgatttc cgacgccctg acctggatcc ccgcactcgg cagcaccgct gggatctttg
     1261 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
     1321 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
     1381 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
     1441 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
     1501 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
     1561 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
     1621 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
     1681 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
     1741 tgtcgtaatg ttatctggag aaaataaaat gttggatatt gataaaagca ccgtggactt
     1801 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
     1861 ggtgcacacc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
     1921 ctgcggatcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
     1981 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
     2041 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
     2101 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
     2161 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
     2221 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
     2281 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
     2341 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
     2401 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
     2461 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
     2521 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
     2581 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
     2641 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
     2701 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
     2761 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
     2821 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
     2881 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
     2941 gcggtcgccc gcacgcgttc gcgtcggact taaaagcggg cggcatcaaa caaatcaagg
     3001 cgctggcgca gacgtatcag atgccgatta tcggctatac gccagaaacc aacggctatc
     3061 cgtataacat gatgctgggc gatgaacata tgcgtcgcga aagcctcgac atgatcaagc
     3121 tggcgatgga tatggcaaaa gagatgaacg cgggttatac gctgatttcc gcggcccacg
     3181 cgggctatct cacgccacct aatgttatct ggggacggct ggcagagaac ctaagcgaac
     3241 tgtgtgagta cgcggaaaac atcggcatgg atctaatcct cgaaccgtta acgccgtatg
     3301 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
     3361 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
     3421 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
     3481 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgagggaa ctgatgcgcg
     3541 atattatcga ccggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
     3601 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
     3661 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
     3721 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
     3781 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
     3841 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
     3901 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
     3961 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
     4021 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
     4081 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
     4141 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
     4201 gaaagcgatt gttgcccgtg gcgcaggcgt ggtgattgtc acgctgggtg aaaacggcag
     4261 cattgcctgg gatggcgcgc agttctggcg tcagactcct gaaccggtga cggttatcga
     4321 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
     4381 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
     4441 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
     4501 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
     4561 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcgac tgctggatga
     4621 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
     4681 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
     4741 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
     4801 cctgcttact gtcagtggtt ttaccgattt tggcatctca caaggcaagg cgacgaaaga
     4861 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatctc
     4921 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
     4981 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
     5041 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
     5101 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
     5161 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
     5221 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta actattgata ataaaagaca
     5281 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcgttgaat
     5341 tttacatccc gtacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
     5401 cagtacggaa tttagcttga cggtgctatc cagcattaaa tactggcaat actctcacgc
     5461 aaaatccgca gcaccgtctc ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt
     5521 tctgattgtg gattcgcctg gcgaaacgtt ccggaaaggc gataaaagag cggcgggatt
     5581 tcatatttcg actctgcacc caccgggtaa ggcaaggcac cgcgcttttg cgcctcttcc
     5641 agcactctgg cggcaatcgg ctgatgaaac tcgacaatca acaccttcga ctgcgcatta
     5701 gcaatcaccg cgcttttcac ttccggcacc gcaccaccgt taagcaaagc cagcaaccgt
     5761 tcagataccc ctgcctgcac cgcgtgcatc actggcgcaa acaccagacc acgcaatact
     5821 tccagcgcct gtgcgccctg gatctggcta ccgccggagt aaagcgtggc gcgaatacgg
     5881 ttgataacat cagcatcgcc gaccactgca ccaacaccct ctggcccaaa tagcttgaag
     5941 caggaaaatg tcgagacatt cgcgccgcat tcacagccga ttcgcgccac cttcatcacc
     6001 gcatagttgt catcggttaa cgctggaaca cctgccgcgc gcaacgttgc cagcacatct
     6061 gccagcccgt agctgtcctg cggctgctgg cgcgtatgct gcaccagcgc cgcatccggt
     6121 tgttgctcgt cgacgacctg cttcagtgcc gacaggtcat tgaaatcaac agtaataagc
     6181 gtcagcccca tctgctcaat aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc
     6241 agaagacgct gccccggttt gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc
     6301 gcgccctgca ccagcgccgc agcctgtgcg tgaaatgcat cagccagcac ctgttccaca
     6361 cgctgggtaa cgcgcggttg attcagtcct ggcgttaagc ctaaatcacc gccagtaaga
     6421 aactcgctgc cggggaaatg gcgacaaatg atatccacca gcgcaaactg cttttgctgc
     6481 gcctcaataa tcgtcaggct ttgcagagga aacgtcttca taggtgcctt ccttaagccg
     6541 gaacgaacag gccaagccag taaagaacat tcagcagaat accggtgatc attactgcca
     6601 cgaccggtgc cgccattttc tgtaccggac ggcccaggga ttcgttgagg aagtaaatgg
     6661 caaccgcgat agagaatccg gtataacccg ccatcttaat tgctgcgaaa atcgaaccga
     6721 ccagcagcgc cacttccatc agcatattca tggcgttacg gatgttatcc gacgcattac
     6781 gcaccgacgg atagcgcccc agccatttgc cgatcgaacg aagcaataaa acttctgccg
     6841 aaataaccac tgcacctaat accgctgcaa ccatcggatt cggcgagaga tagcccaccg
     6901 cataaacaaa ggtaaagccc gcaactgcat acacacccgt tgctaacgcg gtggtggcaa
     6961 tcaacggcac aacccccagt ccgcgcataa attctgccag agccgcctga ttaatcagcg
     7021 tttgcgattg ttccggcgtt acgcctgcgg aatatgcttt ctccagtgtg aagatcgaca
     7081 cttcactgcc agcaaaaatc ttcatgctgg caacggcggc aatcaatgct cccacgatgg
     7141 cgatataggg taagtttttg ataatccgtg acgtgcgttc ttcaaacacc gaaagcccgc
     7201 tggcatcaat gtcattttca tcacgatgac gcaggtcgtg agttatcgcg atccccagca
     7261 gcatcaccat gccaataaag atttcgatgg attcagggtt aagatgtggg aaatagcgca
     7321 cgactaccac acgggtcatc agtaccacca cggcggcgat cagactttgc ttccagccaa
     7381 actggtagaa aatcgccacc agcgggaaca gtgcaaaagc tgaaaccacc ggcgagctta
     7441 attcccctag gctacctaat acatccaccg gcagcgcggt cagcagctgg tttactggca
     7501 acaggcaagt aaggatcaac acgccccaga tagcgccaag accaaacgcc atcaggctat
     7561 ttatcgccag gacgccgagg atatcggtag gaagaaagag taaccatgcg ttgagcagcc
     7621 cggttttcag ggtaaaagag atccccaccg aggccacaaa accaatgctc agaccaaaag
     7681 cgatactccc cgcctcgcga cggttcatat agccttcaat cagttgcggc aggatcgggc
     7741 ggatgccgtc atgaaaaaca gccgccgagc gatgcgccag aagcgatgtc atacccgtca
     7801 ggcacgccac cacgataatc tgaatataca gatccatagt cgtgcctttt atttcagatg
     7861 gttaatcagc atcggaatcg cgtgttcgac gtgctcaacg gaaaggccaa acgccacttt
     7921 cccttcggcg atcattttgg cgatatgctc gtctttcgct ttaatgcccg gcttggcaat
     7981 agtgcaactt ttgttatagc caatcaccgc gatggcaatc gacaatgccg cgcccgcacc
     8041 ggtattacac gcgccgatgt aataatccag ttgcccggat ttcaccttca ttgccgcttc
     8101 catgtcgttg tgaatgaaaa cttcaaaaca gccaggagcc gtcgcttcaa tagttttttt
     8161 aatctgctca cgctgtaagc ctgcaacgcc aatctttttc atgagtctgt ccttattgga
     8221 aaaattgaga gggattttca cgtaacatca catccacatc ggcctgactg aatcctgact
     8281 ggcgcaattg cggaataaag gtggtcaata aatagtcata gccataacca ccgttggctt
     8341 ttaaatggga gcggcgcgta atatccatcg acagcatgac gcggttcagc aacccacggt
     8401 cgcgtagcgc gtggagcatc gcaatgcgct tttcgtccgg gtagtaactg ttcttgccga
     8461 tggtgtcgaa ctgcacatac gcgccgagat cgatcatctt caaaatgttg tcgaggttgt
     8521 ctttcagatc gcagtgacca acggtgacgc gcgaaagatc aactccgtgg gcttgcaaca
     8581 gagccagttg ttccagcccc atggtgctga acgacgtatg cgtggagatc gggcgtccgg
     8641 tctggttatg cgccagcgca gcggcaataa ataccttctc ttccagcggc gtaatctttc
     8701 cttcgctgga gccgatctcc gcgatgatcc cggctttcag ctccgtgcca tcgataccct
     8761 gttcaatttc atcgaccatc tcctgcgcca gttcctgcac gctgcgggtc accacatgtt
     8821 ccgggaaaaa cgcatcctgg taataaccag tacaggccac cacgttgatc ccggtttcgc
     8881 gcattacatc gagcataaat tgcgcattgc gccccatgta acggttggtc atctcaatca
     8941 cattacgtac accccgggtc atcaggtcgt tcatctcctg gcaaatgaac gcatactgat
     9001 caaggcggca gtccacgttg tttttaaagc cggagagatc aatatgcaga tgctcatggg
     9061 cgagggtgta acccgtcgga tcaaaactca tagggtgtct cctgcaaacc ggagggagga
     9121 aagaaaagag cgaccatttt gtggcggtgg cgcgcggaaa aattcacaca cggtagcccc
     9181 cacatcagaa agcgtggtgc gcacaccgag ctgcgtagcg atcatccctt gctgataaac
     9241 cagcactggc accacttcgc gggtatggtg gctgtgacca atggtcggat cgttgccgtg
     9301 atccgccatc acgaccaggc aatcatctgg ctgcatcgcc tcaacaagcc gggcaaggtt
     9361 acggtcaacg acctgcaaac gttcggcata acgtgcgacg tcttctgcat gaccagcgag
     9421 gtcggtttcc tgaatgttgg tgcaaataaa cgccgtcgga tgggtgttaa attcgttgag
     9481 ggtgatatcc ataatccgct ggctatccac cagattttgc cagctcacgc cataaggatt
     9541 gttgacgata tctgccacct tacccaccag cacggttggc acgcctgctt catacagttt
     9601 ttgtggcacc tgcacttttt catcgacgcc atagcccata tgcacgacct ggaaaccgtt
     9661 gtcataagcg ccagaacgcg gcgcattaat cccaataaag cacccttctt tgctttctgc
     9721 ggcatcgaga atgcgttgac tgtcggttaa caggccgcca aatgtaatga cccgaccgat
     9781 ctgtacctgc tcacgcacaa tacgaccaat tttgattgcg tcgtcaaaag agatcacaga
     9841 gagattggcg gtaatgttat agacctggcc taaatccgcc tcgagattat cgccaatcgc
     9901 aaccgactga ttgccccaca gaaattgcag atcatcgcca cggcgctcca cctgccaacc
     9961 ggcggatact aatgcctgct caacacgatc aatcacatcg cgaaagggca tccgcagcgg
    10021 cggtaacggg cgcgtgccta aaatttcctg atgccccata aaggtatcgc caccttcatg
    10081 ttgcagctct gccacgcccc aggttgcgga gtctgacggc tgcatatcgc ctggcgcata
    10141 acccaatgcg ttgattagcc ccagcttctc cagcgttggt agctgcaaat gcggcaactg
    10201 gctcaggatg tgaccacagg tattcgcccc ggcatcctgc ggacgcacca gcgtgacatc
    10261 tttcattgcg cctacgccaa agctatcaat cactaacacc acaaatcgcg ccatttacgc
    10321 ctccagaggg ttgccgagac tgtcataacg accaacgatt ttcggttcgc cacggtgaat
    10381 accggacacc agcaccacat cgctacgggt gacaaaaatc tgcgtgcgga aacagagcac
    10441 cactgcactg ctcaccggat actcgcctgc cagcggcagg gtgtagtcga tactgctgtc
    10501 atccacagtt ttgagattgg tttcagtaat cttttgattt tctggcgtaa acaccagcgc
    10561 atgttgcgca tgaccacgac ggtagtaacc accgccgtag cagtagctgt cgccacggaa
    10621 atgatgggag atttcactta accagagcat cgcgatacgt tcaggctgat cgccctgctg
    10681 gtttgccgga atagtgcccg tcagcgcatg accgggttcg gcatgagtca caccgtattc
    10741 cgccagcaat ggcagcgaag tgcagctggt cgctgaaggc gcgttcagtt gctcaagtgc
    10801 aataccagat ttcgccagtt gatcccgtgc ctgtatcagc gtgtgaagat tcggtgtcgg
    10861 caaaactttt ccggcagcct catcccaaag caggcaaggg aaatgggtaa gtccggctaa
    10921 atgcagcccc ggcagtttct taatttcagc aaccacctcg tgcagcgaat gctgtacaaa
    10981 accgctctcc tgccccggat aaagaaaatc atcgtcgcta taaactttga gcagcacaaa
    11041 ctgcacacgt ccggccttca ccgctgccgc agaaacttcc cgcgctttgt cgagagtaaa
    11101 cacggtgatg acgtcggtgc cctgttcaac ggcgtcagca acctgatgac aagggatttg
    11161 taccagatgc ccctgatgcg ccaccgacaa accagcgcgg cgcatgactc gcgcctcttt
    11221 gtaatccacc gccacaatgc cgctgtaacc taatgccagc aatttttccg ccagccatgg
    11281 gttacggcca aactgcttgg tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc
    11341 aatcagtcgc ttaccgtttt ccagtacctg atccacgtcg atcacccagc tgtccggggc
    11401 gatcttgccc tgctgccaca aacttagtgc ggcggaaatc agcgccgggt tctggcgttt
    11461 caatgcttct acaaacatct tgttgcctcg tttctgttcc ttgaattaaa tattcatttt
    11521 tttgaatatt taggttgtgc gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca
    11581 gccatccata atccataaag gttggccagc aaatagcctt cttcacacgg gttaacttcc
    11641 agtgcgaatt ccttcaacaa cacctgatgc aattgcacaa cggcttgcca gtggctggat
    11701 tgcgccagct ctgccagcaa ttcgttatcc agcggctcta tttcttcacc acggcgactg
    11761 cgcatcagtg cactcgccat atgtgtcatc gccatcgttc cttgctcact gcgcaccggc
    11821 agatggcact ctgtttccag tacgttgacg acctgcatca tgcctttgca gatgtcctta
    11881 tcaataacgc ctgcctcgca aagcaggttg agtctggttt ccatatcaga tgcctttcgt
    11941 ttaataactc ggttcctgtt ccccactcac tacccgttgt tgatgggcga gcagggcgtg
    12001 tttatctaca acggcacgca gcagttgttg cattgggtaa tcatcgactc gcgtcaggac
    12061 cacggcttcg gtggcctgta aaaatcgcgg atcgtctgtg agcggcgtcg cctctaatcc
    12121 cagcatggtc agttcgtttt ccgccaccac gttccagatc accgcatcga catcgccttt
    12181 aacaatgcgt tgtaaactct cgtgataaga gagatcgact cgttccacat cactaccgcc
    12241 aaaaaaaaca tcggtcatga ttttctgatc cgccgaacgg ttatccagcc ccacgcgctt
    12301 cacgtttgcg gactcgcctt tacggcaaat caactggtgc tcgccaacgt aggtgtgcgg
    12361 ccccaactcc agcgcgaggc ataacccttt ttgcgtgaga taactttccg ccgccagtcg
    12421 ggaaaccacc gccatgtcat acacgccgtt aagtaaacac tccacgcgaa tatccgcgcc
    12481 gcgcatatgc gcatagtaaa aaggaatgcc atcaaactga gctttcagcc cgctcgctaa
    12541 accttcgtac aagcgggtat agggcaaagg catcgcacat accacgttgt tgatatccac
    12601 atgactcagc aatgctttgt tatccatctc gaccagataa ctgccattgc gcccacggcg
    12661 ttcaatccgt atcgctccgc ttgattccag cgttttcaac gcggcctgcg tcaggccaac
    12721 cgatgaacgg cattcagttg ccagctcatc tatggttttc aacctattac cgcatttttc
    12781 acccaacaaa taacgggcca gcgtcgtgat aacgacgcct tcttttttga taaacgttcg
    12841 acgcata
//
LOCUS       DAZFAH010000137        12751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFAH010000137 DAZFAH010000000
VERSION     DAZFAH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.137"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..732)
                     /locus_tag="ACHJWB_003447"
     CDS             complement(76..732)
                     /locus_tag="ACHJWB_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO4315021.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            complement(729..1016)
                     /locus_tag="ACHJWB_003448"
     CDS             complement(729..1016)
                     /locus_tag="ACHJWB_003448"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4315022.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            complement(1153..1857)
                     /locus_tag="ACHJWB_003449"
     CDS             complement(1153..1857)
                     /locus_tag="ACHJWB_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4315023.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1971..2204
                     /locus_tag="ACHJWB_003450"
     CDS             1971..2204
                     /locus_tag="ACHJWB_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4315024.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            2343..2639
                     /locus_tag="ACHJWB_003451"
     CDS             2343..2639
                     /locus_tag="ACHJWB_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4315025.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            2672..3610
                     /locus_tag="ACHJWB_003452"
     CDS             2672..3610
                     /locus_tag="ACHJWB_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4315026.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNTSEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQTENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAVVTASKPKLDLTNTDWIYGVDL"
     gene            3607..4308
                     /locus_tag="ACHJWB_003453"
     CDS             3607..4308
                     /locus_tag="ACHJWB_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4315027.1"
                     /translation="MKNIAAQMVNFDREQLRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQDRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            4305..4595
                     /locus_tag="ACHJWB_003454"
     CDS             4305..4595
                     /locus_tag="ACHJWB_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4315028.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            4666..4944
                     /locus_tag="ACHJWB_003455"
     CDS             4666..4944
                     /locus_tag="ACHJWB_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315029.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            5077..5292
                     /locus_tag="ACHJWB_003456"
     CDS             5077..5292
                     /locus_tag="ACHJWB_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315030.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            5303..5539
                     /locus_tag="ACHJWB_003457"
     CDS             5303..5539
                     /locus_tag="ACHJWB_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO4315031.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            5496..5942
                     /locus_tag="ACHJWB_003458"
     CDS             5496..5942
                     /locus_tag="ACHJWB_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4315032.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            5939..6466
                     /locus_tag="ACHJWB_003459"
     CDS             5939..6466
                     /locus_tag="ACHJWB_003459"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4315033.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            6463..6645
                     /locus_tag="ACHJWB_003460"
     CDS             6463..6645
                     /locus_tag="ACHJWB_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4315034.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            6920..7654
                     /locus_tag="ACHJWB_003461"
     CDS             6920..7654
                     /locus_tag="ACHJWB_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO4315035.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            7729..8451
                     /locus_tag="ACHJWB_003462"
     CDS             7729..8451
                     /locus_tag="ACHJWB_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4315036.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
     gene            8451..9056
                     /locus_tag="ACHJWB_003463"
     CDS             8451..9056
                     /locus_tag="ACHJWB_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4315037.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            9053..9247
                     /locus_tag="ACHJWB_003464"
     CDS             9053..9247
                     /locus_tag="ACHJWB_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO4315038.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            9240..9674
                     /locus_tag="ACHJWB_003465"
     CDS             9240..9674
                     /locus_tag="ACHJWB_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4315039.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            10181..11128
                     /gene="stxA1a"
                     /locus_tag="ACHJWB_003466"
     CDS             10181..11128
                     /gene="stxA1a"
                     /locus_tag="ACHJWB_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4315040.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            11138..11407
                     /gene="stxB1a"
                     /locus_tag="ACHJWB_003467"
     CDS             11138..11407
                     /gene="stxB1a"
                     /locus_tag="ACHJWB_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4315041.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            11907..>12751
                     /locus_tag="ACHJWB_003468"
     CDS             11907..>12751
                     /locus_tag="ACHJWB_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4315042.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCV"
BASE COUNT         3553 a         2815 c         3215 g         3168 t
ORIGIN      
        1 ctttttttgc tgttgaatcg ccttctagcc gtgtcataac caagcgttca acttttttgc
       61 taatgagttc aattttcatg gtaataataa gtaatttccg tcagaagtca tttcgtcatt
      121 caggttgcta gccgctgtga aacttgatga caagttactt atcaaagaaa aaacattccg
      181 tttttctttg catttgccat taccccttac tgcgctgaac aacatttttg aaaagtaaga
      241 ttctgtagcc ttttcagcga aataagcaga aggtgatgtg acggctgact cacaccgagc
      301 aatgacgcta gtcatggctg tttcttcttc ctctgcgtca actaggctcc attcaattgc
      361 gctcatatct tgaattaaga tatctgcagg aattccaagt ccgtcatgta gtctacggat
      421 cattgaaaga cttaatggac gcttacgatt taagacctca gatactttag atgctgagcc
      481 aatgtattgt ttcatatcgg cataagagag gccttgttga tccatacgga acttgattgc
      541 ttctatggga tctggcttgt ccatagggaa ctcgcgtgac tcatagtgct cgataagcaa
      601 gcctagtagt tcaaactcat caaaatcttc agttccaggt tgtaaatcac tagacgcaag
      661 ttcaatgagc ctttccatag ctgcagcatg ctcttcacta tttttaatga tgcgccagct
      721 agttcgattc atttaagcct ccacttgtcg tactctgcat gagttccaac tctttcaact
      781 atgaccattc ctgcctggta aacaacttgg acaacgagcc gatagttatt gccttttata
      841 ttaaaaatta ccctgttgtt aggaaggaag tcggcagaat taaatctatt ccgtatgtct
      901 tgagtcgttt tccagttttc tcttatcact tcatcatacc aagagtctaa agcccccttg
      961 gcctgattgt gcttccggct aaaactgtga agcttctcta cgtttaagac cttcataatc
     1021 cgattttgtt ccctctttgg gaattataga ttcatttccc ataaagggaa tctgtatttt
     1081 tatccagaaa tgctatgtac atcacccaaa cgtctcttca ggccactggt taccagctat
     1141 gtgacgatga agtcacgaac ttttcagcca ctcccttgcc tcgatgtcat ccagatggcg
     1201 agattgcttc agaataccag ccacatactc cacctttgct acttgatgat aaggcaacgt
     1261 tattggcctg tggtcctggt tgatgcttgt aaattggtat tctccgtctc tgtcatagcc
     1321 aagaactttg atcatgttgt gtccttcaac agttctgaca aacacctcat cacccgggaa
     1381 tactttggtg ttaggctcaa tgagtacata ttctcctgat tttattctgg gccacatgct
     1441 gtctcctttc acacgaagac caaaggcatc tggatcatcg ctataaattt tgagccaccc
     1501 atcgcgctct tcggtcatct cgatggcacc atcaacacca agaattgcct caccaaccac
     1561 gcgcactaac ccttttctta cctgaccgac aaaagttaaa gaatctgagc atgatgcaat
     1621 tggtgttaca tcatgtacca aatcaagcca cccattaggt aacccaagcg cggcttcaaa
     1681 ttttcttgct agtttatccc ctatgtttcg agtgcttttt tcaccggaga cttgcgtgag
     1741 ttgagaaggg ctaaccccaa gcttatcggc aaagcttgca ttagtgttac ccgcgatttt
     1801 tttatgctca tctagcaaaa acgccagatt cgatttgcga atatctttgt tttccatctc
     1861 acaattttcc ctctatttag caaatggata aatacgcaat atgataaatt tacattgcgg
     1921 atgatttatc aaaatggtaa agttgttctg tgtgataaac ggaggcacta atgagtaatg
     1981 aactactacg ctggcgaaaa gaggcttcta gtgaggaatg gaagcgactc gccgcattag
     2041 cgaaaacttc agttggctat cttgaccaga ttgcatatgg atttcgaaga gcttcccctg
     2101 ataaagcgaa tgcaatcgaa gaagctactc gtaatttcac gggttataaa cctgtgaaaa
     2161 aggaaaacct agtgttcgta tcgcgtagag catcggctgc ataagtaaca ccgctatttt
     2221 cacaatggac attcgtccta cgtcgctgac aaagcgagtc ccaatatatc tgaccaacta
     2281 aggccatatg cgtttccacg catacctttc aactagctat tcactattgg aaatcttaag
     2341 aaatggaaca aacaagttac agcaaactat cacagcgaga aattgatcgc gctgaaactg
     2401 atttactcat caacctgtca acgcttaccc agcgcggtct ggcaaagatg attggctgtc
     2461 atgaatcgaa gataagcaga acagactgga ggtttatagc ttcggtcttg tgtgcttttg
     2521 gcatggcatc agacatcagt ccgattagca gagcttttaa gtatgcgctt gatgaaatca
     2581 caaagaaaaa atccccggtg gccgccgggg actctaagca aattgatatg caattctgag
     2641 ggaattactg gatcaatcca caggagtcat tatgacaaat acagcaaaaa tactcaactt
     2701 cggcagaggt aactttgccg aacaggagcg taatgtggca gatctcgatg atggttacgc
     2761 cagactatca aatatgctgc ttgaggctta ttcaggcgca gatctgacca agcgacagtt
     2821 taaagtgctg cttgccattc tgcgtaaaac ctatgggtgg aataaaccaa tggacagaat
     2881 caccgattct caacttagcg agattacaaa gttacccgtc aaacggtgca atgaagccaa
     2941 gttagaactc gtcagaatga atattatcaa gcagcaaggc ggcatgtttg gaccaaataa
     3001 aaacacctca gaatggtgca tccctcaaaa cgagggaggt tcccctaaaa tgagggacat
     3061 ccctcaaaac gagggaaaat cccctaaaac gagggataaa acatccctca aattagggga
     3121 ttgctatccc tcaaaacagg gggacacaaa agacactatt acaaaagaaa aaagaaaaga
     3181 ttattcgtcc gagaattctg gcgaatcctc tgaccagaca gaaaacgatc tttctgtggt
     3241 taaaccggat gctgcaattc agagcggcag caagtgggga acagcagaag acctgaccgc
     3301 cgcagagtgg atgtttgaca tggtgaagac catcgcacca tcagccagaa aaccgaattt
     3361 tgctgggtgg gctaacgata tccgcctgat gcgtgaacgt gacggacgta accaccgcga
     3421 catgtgcgtg ctgttccgct gggcatgcca ggacaacttc tggtccggta acgtgctgag
     3481 cccggccaaa ctccgcgaca agtggaccca gctcgaaatc aaccgtaaca agcaacaggc
     3541 agtcgtgaca gccagcaaac caaaactcga cctgacaaac acagactgga tttacggggt
     3601 ggatctatga aaaacatcgc cgcacagatg gttaactttg accgtgagca gttgcgtcgg
     3661 atcgccaaca acatgccgga acagtacgac gaaaagccgc aggtacagca ggtagcgcag
     3721 atcatcaacg gtgtgttcag ccagttactg gcaactttcc cggcgagtct ggctaaccgt
     3781 gaccagaacg aactgaacga aatccgccgc cagtgggttc tggctttccg ggaaaacggg
     3841 atcaccacaa tggaacaggt taacgcagga atgcgcgtag cccgtcggca ggatcgacca
     3901 tttctgccat cacccgggca gtttgttgca tggtgccggg aagaagcatc cgttatcgcc
     3961 ggactgccaa acgtcagcga gctggttgat atggtttacg agtattgccg gaagcgaggc
     4021 ctgtatccgg atgcggagtc ttatccgtgg aaatcaaacg cgcactactg gctggttacc
     4081 aacctgtatc agaacatgcg ggccaatgcg cttactgatg cggaattacg gcgcaaggct
     4141 gccgatgaac tgacctgtat gacagcacga attaaccgtg gtgagacgat acctgaacca
     4201 gtaaaacaac ttcctgtcat gggcggcaga cctctaaatc gagcacaggc tctggcgaag
     4261 atcgcagaaa ttaaagctaa gttcggactg aaaggagcaa gtgtatgacg ggcaaagagg
     4321 caattattca ttacctcggg acgcataaga acttctgtgc acaggacgtt tccgcggtaa
     4381 caggcgcaac cgtaaccagc ataaatcagg ctgcggctaa aatggcgcgg gcaggaatcc
     4441 tggtcattga tggtaaggtc tggcgaacgg tgtattaccg gtttgctacc agggaagaac
     4501 gggaaggaaa gatgagcacg aacctgattt ttaaggagtg tcgccagagt gcagcgatga
     4561 aacgggtatt ggcggtatat ggagttaaaa gatgaccatc tacatcactg agctaataac
     4621 aggcctgctg gtaatcgcag gcctttttat ttgggggaga gggaagtgaa cgatagctac
     4681 cgacagtttg aaaactggtg gtcaaaagac aaaagccagt tcacgggaga cgatgaatta
     4741 aaagagtttg cctgggtgat atggcaggca tcgcgctctg ctattgaact ggatatcgac
     4801 tggcccgaat cgaatgacga cttttggaaa gatggtgaag aaggtgctta tgcgatgggt
     4861 tatgaggatg ggcgtgacaa aacggtaatt gcagtaatga aagccatcag ggccgcagga
     4921 atcaaagaaa agaatttcga ttaagcaaat atcacttcaa taaatcgctt ttaaggcatc
     4981 acaatcgctc tgtagcgagg taaacgcgtg caaggcatgc caataagcag cgagaatgaa
     5041 aaatgcgtca gaatgcgttt gaggaggttt taagaaatga gtacgatagc tgagcttgtc
     5101 agggctaatt ttcgtgaaga gttggtgcgt tggtatcggt atcgttcatc gtccagtttg
     5161 ccgcttgatg agttgtatga gcattcacct gccgcacgac gctatccgcg tgaccgtgtt
     5221 cttcgacggt tgttcaaact caacaatgag tttcagcgca acagaattat ccggagtctg
     5281 gatttaaagt gaaggagtga gcatgagcga cctatcatta acccagccaa agctaaaaga
     5341 atgtccgttt tgcggcggta atgctcgtct gtgggttgag gccggaataa atattgatgt
     5401 gtggggctat gcagaatgtg acctctgtga agccaggggg gcatgggcac catcagttgc
     5461 tgcggcggct gaaaaatgga accggagagc aggagatgaa gcaaaccttt ctgcttcgca
     5521 acgaagcaat cagaaataac gccatagacg ccattctctc actacccatc gacgacaagt
     5581 caccccacga agtccacgtt aaagaaccca agcgcagcaa agcgcagaat gaccgtatgt
     5641 ggccgatgct gaacgatgtt tcgcgtcagg tgctatggca tggtcaacgg ctggcgccgg
     5701 aagactggaa agacctgttc actgccctgt ggcttaagac caaaaaactg gagcaacgaa
     5761 gtgtgcctgg tatcgacggt ggcgttgtca tgcttggcgt gcgtaccagc aaaatgcgga
     5821 aggccagcat gactgagctt atcgaaatca tgttctggtt cggctcagag cgcaacgtgc
     5881 ggtggagtga tgactcccgg cgagagtatg aatggtcaca acgaaaaggt agggctgcat
     5941 gactatcaaa tcaaatacgc cagcacacga caaggactgc tggcaaacgc cgctttggct
     6001 ttttgatgca ctggatattg agtttggatt ctggctggat tcggcagcga gcgacaaaaa
     6061 tgctctgtgc gctcactggc taactgaggc cgacgacgcg ctcaattctg agtgggtaag
     6121 ccacggtgca atctggaata acccaccgta cagcaatatc aggccgtggg tggaaaaagc
     6181 cgctgagcag tgcatacaac agcgacagac ggtagttatg cttgtgccag aggatatgtc
     6241 agtcggatgg ttcagcaagg ctctggagag tgttgacgaa gttcgcatca tcactgatgg
     6301 acggattaat tttatcgaac catcgacagg gctggagaag aagggaaaca gcaaaggctc
     6361 catgctgctg atttggcgac cgttcatcag tcctcgacga atgtttacta ctgtatccaa
     6421 agcggcattg atggcgatcg ggcagggcgt caggagggcg gcatgaggcg acagcgacga
     6481 agtatcaccg acatcatctg cgaaaactgc aaataccttc caacgaaacg ctccagaaat
     6541 aaacgcaagc caatcccaaa agaatctgac gtaaaaacct tcaattacac ggctcacctg
     6601 tgggatatcc ggtggctaag acatcgtgcg aggaaaacaa ggtgattgac gcgatgattt
     6661 attcggggct atattcctca cgcgccagca aaatctggcg tcgggattgg cgtcctggat
     6721 agagaccgcg acagatacac gccgcgagcg tgttttttat tgtcgtatgc acgcgcacat
     6781 ctgaattatg gtgggctgtg tgggggcgga gagatccgcg ccggtcggtt tcccggttac
     6841 gccaaccctg cacagttcac caccagacga ttggcgtcgt cggtggtgag ttattaagaa
     6901 accaccagag ggcgtcatta tgacaactca aatttctgtt gaaactctct ccccgatcac
     6961 ccataaccag attcctgtta ttaccaccga acttttggcg cagctttacg gcactgagcc
     7021 ggtgcgtatt cgccagaatc atcatgagaa caaagtacgc ttcgttgaag ggaaacactt
     7081 tttcaaagtt gttggtaatg accttaaaga attgcgggta gctttaaact actcacaaaa
     7141 tttgcgggtt actttaagta actcacaaaa tttgcaacca tctttaagag ggttacaaat
     7201 ttccccgaaa gcccgctccc tcatactctg gacagaacga ggcgcagccc gtcacgcaaa
     7261 aatgctcgaa actgatcagg cgtgggatgt gttcgaaaaa ctggaagact gctatttcag
     7321 ccagtgcgag aaaaatactg gcaaacaaga gaagaagctc aacgggcttt ccgcaaaaga
     7381 aacagacagc cttgtatggc tgtgggatta tgccaaccgc tcacaggcat tgttccgtga
     7441 gttgtatccc gcattaaaac tgattcagtc tggctattcc ggcatatgcc acgactacgg
     7501 ctatgagttc tcgtatatca tcgggagggc gaggggcgtt ttaattaatc acacgcggga
     7561 tatagatatt tatgagcctg acgggccgac gaaccttctg gcatgggaaa ggcttaagaa
     7621 caaagagttg ccgccttcac tgcatcgcta ctgacaattg acaacttaac aaacccagct
     7681 tcggctgggt tttttattgc tgaattttca atgtgagagg acatgacaat gaatgagctg
     7741 ataaatagca atgccatcaa aatgacaagc attgaaatcg ctgagttggt gggaagccgt
     7801 catgacaagg tgaaacaatc cattgaacga ctggcggttc gaggtgtgat ccgaaatccc
     7861 ccaatggtgg ttttcgaaaa aatcaataac ttaggattac ttcgtggcgt agaggcttac
     7921 gtttttgagg gcgaacaagg taagcgcgac agcattattg tcgttgccca gttgtcgccg
     7981 gaattcaccg ctcgccttgt tgaccgctgg cgagaactcg aaggggcaac cgcgaaaata
     8041 ccacaaacct tttctgaggc attgcgcctt gcggccgacc ttgaagacca gaaggctgaa
     8101 ctggagaaac agcttgctct cgcagcacct aaagttgagt ttgccgatcg cgttggcgag
     8161 gccagcggaa ttttgattgg aaactttgca aaggttgttg gtattggtcc aaacaaactg
     8221 tttgcgtgga tgcgcgatca caaaatcctt attgcttcag gctcccggcg caatgtgcca
     8281 atgcaggaat atatggatcg tggctatttc acagtgaaag aaacagcggt caatacaaat
     8341 cacggaatac agatatcgtt caccacaaaa atcaccgggc gtggtcaaca gtggctgacc
     8401 agaaagctgc tcgataacgg aatgctgaaa gtaacaaggg aggctgctta atggctaaac
     8461 cagcgcgaag gaaatgcaaa atatgcaagg aatggtttca cccggcattc tcaaatcagt
     8521 ggtggtgctg cccggaacac ggaactcaat tagcactcga acgacgaagt aaagaacgcg
     8581 aaaaagcgga aaaagcagca gagaagaaac gacgacgaga ggagcagaaa cagaaagata
     8641 aactgaagat tcgaaaactc gccttaaagc cccgcagtta ctggattaaa caagcccaac
     8701 aagccgtaaa cgccttcatc agagaaagag accgcgactt accatgtatc tcgtgcggaa
     8761 cgctcacgtc tgctcagtgg gatgccggac attaccggac aactgctgcg acacctcaac
     8821 tccgatttga tgaacgcaat attcacaagc aatgcgtggt gtgcaaccag cacaaaagcg
     8881 gaaatctcgt tccgtatcgc gtcgaactga ttaaccgcat cgggcaggaa gcagtagacg
     8941 aaatcgaatc gaaccataac cgccatcgct ggactgtcga agaatgcagg gcgatcaagg
     9001 cgaagtatca gcagaaactt aaagacctgc gaaacagcag aagtgaggcc gcatgacgtt
     9061 cacagtaaaa accattcctg acatgctcct tgaggcatat ggaaatcaga ccgaggtagc
     9121 ccgaatactg aactgcaatc gtgccacagt cagaaaatac attggcgata aagaagggaa
     9181 aaagcacgcc gtcgtcaatg gcgtccttat ggttcatcgc ggatggggta aagatactga
     9241 tgcgtgatat ccggcaggtt cttgagcgct ggggggcatg ggcggcaaat aaccatgagg
     9301 atgttacatg gtcgcccatt gctgccggat ttaagggact gatccccgaa aaagtaaaat
     9361 cacgtccaca gtgttgtgac gatgacgcga tgattatatg cgggtgtatg gctcgcctta
     9421 acaggaacaa cagcgatctg catgacttgc tggttgatta ttacgtgttg ggggagacgt
     9481 tcatggcgct ggcacggaaa catgggtgct ctgacacctg tataggtaaa cgccttcaca
     9541 aagcggaggg gattgttgaa ggcatgctga tgatgctggg agtgaggctt gagatggatc
     9601 ggtatgttga gcgtgaattg ccgggaggga gaacctctgt attttatcag cgaaaaaata
     9661 gtttacgatc gtaaaaatct gcatatcatg ataagagtgg ttacattgcc acgctgctta
     9721 acccgccgat gcgcgggttt ttttgtaccc agaatcctgt gagctatacg gaaagtacac
     9781 agaaaggaag gtgcgaccac aattaataac aaaatcttaa aaattgcaca tggcactatt
     9841 agttttctaa atattgtgta ttttttgtat tgcaggatga ccctgtaacg aagtttgcgt
     9901 aacagcattt tgctctacga gtttgccagc ctcccccagt ggctggcttt tttatgtccg
     9961 taacatcctg tgtatcaata aatgttgtta tctacgtacg tcaagtagtc gcatgagatc
    10021 tgaccagata tgttaaggtt gcagctctct ttgaatatga ttatcatttt cattacgtta
    10081 ttgttacgtt tatccggtgc gccgtaaaac gccgtccttc agggcgtgga ggatgtcaag
    10141 aatatagtta tcgtatggtg ctcaaggagt attgtgtaat atgaaaataa ttatttttag
    10201 agtgctaact tttttctttg ttatcttttc agttaatgtg gtggcgaagg aatttacctt
    10261 agacttctcg actgcaaaga cgtatgtaga ttcgctgaat gtcattcgct ctgcaatagg
    10321 tactccatta cagactattt catcaggagg tacgtcttta ctgatgattg atagtggcac
    10381 aggggataat ttgtttgcag ttgatgtcag agggatagat ccagaggaag ggcggtttaa
    10441 taatctacgg cttattgttg aacgaaataa tttatatgtg acaggatttg ttaacaggac
    10501 aaataatgtt ttttatcgct ttgctgattt ttcacatgtt acctttccag gtacaacagc
    10561 ggttacattg tctggtgaca gtagctatac cacgttacag cgtgttgcag ggatcagtcg
    10621 tacggggatg cagataaatc gccattcgtt gactacttct tatctggatt taatgtcgca
    10681 tagtggaacc tcactgacgc agtctgtggc aagagcgatg ttacggtttg ttactgtgac
    10741 agctgaagct ttacgttttc ggcaaataca gaggggattt cgtacaacac tggatgatct
    10801 cagtgggcgt tcttatgtaa tgactgctga agatgttgat cttacattga actggggaag
    10861 gttgagtagc gtcctgcctg actatcatgg acaagactct gttcgtgtag gaagaatttc
    10921 ttttggaagc attaatgcaa ttctgggaag cgtggcatta atactgaatt gtcatcatca
    10981 tgcatcgcga gttgccagaa tggcatctga tgagtttcct tctatgtgtc cggcagatgg
    11041 aagagtccgt gggattacgc acaataaaat attgtgggat tcatccactc tgggggcaat
    11101 tctgatgcgc agaactatta gcagttgagg gggtaaaatg aaaaaaacat tattaatagc
    11161 tgcatcgctt tcattttttt cagcaagtgc gctggcgacg cctgattgtg taactggaaa
    11221 ggtggagtat acaaaatata atgatgacga tacctttaca gttaaagtgg gtgataaaga
    11281 attatttacc aacagatgga atcttcagtc tcttcttctc agtgcgcaaa ttacggggat
    11341 gactgtaacc attaaaacta atgcctgtca taatggaggg ggattcagcg aagttatttt
    11401 tcgttgactc agaatagctc agtgaaaata gcaggcggag attcataaat gttaaataca
    11461 tctcaattca gtcagttgtt gccggtctga taatagatgt gttagaaaat ttctgcatgg
    11521 tgaatccccc tgtgcggagg ggcgactggt gaacggtatg atctctttga tgatcgtaag
    11581 cgagaatacg cgggtttggt ggcaccaggc cgaactcacc gggaggcacc cggcatcatg
    11641 ctgtatacag agattaggca tatatccagg ctcctcatcg caggagcctt tttacatgca
    11701 aaaaaaagcc cgagtgggtt cgggcaacag catgagatac ttgcattgtc atttttatcg
    11761 tgcggatttt aaccaggatt cataaggctg cgcaactgcg cggccttttt cgtatttcgg
    11821 gctgtagtcc ccgtgtgtca ttcaggcttc cggactacag cccactccat atctgattta
    11881 atacactatc ccggccggga ggaataatga catttaaaca ttatgatgtt gtcagggcgg
    11941 cgtcgccgtc agaccttgcg gaaaagctga cacacaaact gaaagagggc tggcagccgt
    12001 ttggtagtcc ggtggccata accccttata ccctgatgca ggccattgcg gcggaaggtg
    12061 atgtcaccac accagtggcg gtgaccggta atgagggtaa ggcggtggct gtcagtgcca
    12121 ccagcgaccc ggagtattac tttgttgtgg ttctggcagg gcagtcaaat ggcatgtcgt
    12181 atggtgaagg tcttccgctg ccggagacat atgaccgtcc ggacccgcgt attaagcagc
    12241 tggcgcgccg cagtacggtg acaccgggcg gtgcagcatg caaatataac gacatcattc
    12301 cggcggacca ttgtctgcat gatgtgcagg acatgagccg tcttaaccat ccgaaagcgg
    12361 acctgtcaaa agggcagtac ggtaccgtgg ggcaggggct gcatatcgcc aaaaaactgc
    12421 tgccgtttat accggcgaat gcgggcattc tgctggttcc gtgctgtcgt ggtggttcag
    12481 cgttcaccac cggagccgat ggcacataca gtgacgcgag tggtgcctcg gagaattcaa
    12541 cccgctgggg tgtggacaag ccgctgtata aggaccttat cggtcgaaca aaagcagcac
    12601 tgaagaagaa tccgaaaaat gtgctgtttg ccgtggtgtg gatgcagggg gaatttgatt
    12661 ttggcggtac gccggcaaat cacgcagcac agtttggtgc gctggttgat aaattccgtg
    12721 cagacctggc ggatatggca ggtcagtgcg t
//
LOCUS       DAZFAH010000138        12644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFAH010000138 DAZFAH010000000
VERSION     DAZFAH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.138"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHJWB_003469"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHJWB_003469"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4315043.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHJWB_003470"
     CDS             complement(1575..1682)
                     /locus_tag="ACHJWB_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315044.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHJWB_003471"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHJWB_003471"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4315045.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHJWB_003472"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHJWB_003472"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4315046.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHJWB_003473"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHJWB_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4315047.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHJWB_003474"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHJWB_003474"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4315048.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHJWB_003475"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHJWB_003475"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4315049.1"
                     /translation="MQNSALKAWLDSSYLSGTNQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHJWB_003476"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHJWB_003476"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4315050.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHJWB_003477"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHJWB_003477"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4315051.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHJWB_003478"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHJWB_003478"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4315052.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHJWB_003479"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHJWB_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO4315053.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3036 a         3230 c         3429 g         2949 t
ORIGIN      
        1 ttacaatttt ttgtgtttag gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacggt
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgcgagtcgc gacggaaagc gtggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attacgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaacg gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcagcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt ggggtgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtagtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga tcgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgaccacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtaccttcgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtta
     3901 ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt ggttgttccg ggactgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgcgcgg caggtctgca tctgcaagag tctatcgccg
     4081 agcagggcgc actgcgcgat gccagcgagt ctgatgttga agtgtctctg gatcgcctga
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcct gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgtcgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcaca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccgctggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgc tgcaacatat tcataacggc cagggcgcgt
     7561 tccgtgtctg ggactccgtt ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgactatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cctctggcga gcagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtg tgcgtaccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcatctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttctacgccg cgcggcctgg tgacaccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca agctgaccgt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc aggctctaac
    11461 tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc
    11581 aagccagttg taggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcga tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatacgaatt tagccattat gcccgataaa ttca
//
LOCUS       DAZFAH010000139        12594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFAH010000139 DAZFAH010000000
VERSION     DAZFAH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.139"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(120..2111)
                     /gene="tkt"
                     /locus_tag="ACHJWB_003480"
     CDS             complement(120..2111)
                     /gene="tkt"
                     /locus_tag="ACHJWB_003480"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4315054.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2389..3147
                     /gene="loiP"
                     /locus_tag="ACHJWB_003481"
                     /gene_synonym="yggG"
     CDS             2389..3147
                     /gene="loiP"
                     /locus_tag="ACHJWB_003481"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4315055.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3203..3946)
                     /locus_tag="ACHJWB_003482"
     CDS             complement(3203..3946)
                     /locus_tag="ACHJWB_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4315056.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3933..5042)
                     /gene="dgaE"
                     /locus_tag="ACHJWB_003483"
     CDS             complement(3933..5042)
                     /gene="dgaE"
                     /locus_tag="ACHJWB_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO4315057.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5046..5906)
                     /locus_tag="ACHJWB_003484"
     CDS             complement(5046..5906)
                     /locus_tag="ACHJWB_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4315058.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5903..6652)
                     /locus_tag="ACHJWB_003485"
     CDS             complement(5903..6652)
                     /locus_tag="ACHJWB_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO4315059.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPVAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6678..7163)
                     /locus_tag="ACHJWB_003486"
     CDS             complement(6678..7163)
                     /locus_tag="ACHJWB_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4315060.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7174..7602)
                     /locus_tag="ACHJWB_003487"
     CDS             complement(7174..7602)
                     /locus_tag="ACHJWB_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4315061.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7721..10519)
                     /gene="dagR"
                     /locus_tag="ACHJWB_003488"
     CDS             complement(7721..10519)
                     /gene="dagR"
                     /locus_tag="ACHJWB_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO4315062.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETVDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10778..11698)
                     /gene="speB"
                     /locus_tag="ACHJWB_003489"
     CDS             complement(10778..11698)
                     /gene="speB"
                     /locus_tag="ACHJWB_003489"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4315063.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11834..12565)
                     /locus_tag="ACHJWB_003490"
     CDS             complement(11834..12565)
                     /locus_tag="ACHJWB_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315064.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         3038 a         3527 c         3180 g         2849 t
ORIGIN      
        1 atttattgca tataaatatt tgtgatctac aacacgcttt atctattgct tgtccgcaaa
       61 cggacatatc aaggtaataa aaaaggtcgc cgaagcgacc tttttacccg aaatgctaat
      121 tacagcagtt cttttgcttt cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac
      181 agcagctctg ccggagcaga ttcaccgaag gtggtcatac cgacgatagc accgttcagg
      241 ccaacatact tgtaccagta gtcagcaata cccgcttcta cagcaacgcg tgcagtaacc
      301 gctttcggca gtacggattc acggtaagca gcatcctgct tgtcaaatgc gtcggtagac
      361 ggcatggaca ccacgcgcgc tttcacgcct tcggcagtca gtttttcgta ggcagcaaca
      421 gccagttcaa cttctgaacc ggtagcgatg aaaatcagtt ccggctgacc ggcgcagtct
      481 ttcagcacat aaccaccgcg cgcgatgttt gccagttgct cttcagttcg ttcctgctgc
      541 gccaggttct gacgggagag gatcagtgcg gtcgggccgt cctgacgctc aacaccgtat
      601 ttccacgcga ccgcggattc aacctggtca cacggacgcc atgtagacat gttcggggtt
      661 acgcgcagag aagcgacctg ctcaaccggc tggtgagtcg ggccgtcttc gcccagaccg
      721 atggagtcgt gggtgtaaac catcacctga cgctgtttca tcagcgcagc catacgtacg
      781 gcgttacgtg cgtattccac gaacatcagg aaggtggagg tgtacggcag gaaaccaccg
      841 tgcagggaga taccgttagc aatcgcggtc ataccgaact cgcgaacacc gtagtggatg
      901 tagttacccg cagcatcttc gttgattgct ttagaaccag accacagggt caggttagac
      961 ggtgccaggt cagcggagcc gccgaggaat tccggcaaca gcgggccgaa cgcttcaata
     1021 gcattctgag acgctttacg gctggcgatt ttcgccggat tagcctgcag tttagcgata
     1081 aactctttcg ctttggcgtc gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat
     1141 tcagccgctt cctgcggata agctttcgcg taagccgcaa acttctcatt ccatgcagat
     1201 tctttcgcct ggcctgcttc tttcgcatcc cactgcgcat agatttcaga cgggatttcg
     1261 aacggtgcgt atttccagcc cagctgttcg cgggtcagag cgatttctgc atcacccagc
     1321 ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg aaccgaaacc gatgatggtt
     1381 ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg
     1441 atggatgccg cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa
     1501 cgcattgcgg tgtcgtcggt gaaccagcct tcaacgtgac catcaataga gataccgttg
     1561 tcatcgtaga acgcaatcag tttacccagc ttcagcgtac ccgccagaga gcaaacttcg
     1621 tgggagatgc cttccatcat gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca
     1681 atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt tttctgcaat cgccataccg
     1741 actgcgttgg caataccctg acccagcgga ccggtggtgg tttccacacc agcggtataa
     1801 cctacttccg ggtggcccgg agttttagag tgcagctgac ggaagttttt cagttcttcc
     1861 atcggcagat cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga gccgtggccg
     1921 ttggacagca cgaagcggtc acggtcagcc caggacggat tctgcgggtt atgtttcagg
     1981 aaatcacgcc acaggacttc ggcaatgtca gccataccca taggggcacc cgggtgaccg
     2041 gatttggctt tctgtactgc gtccatgctc agcgcacgaa tagcattggc aagctcttta
     2101 cgtgaggaca ttttgactcc agatcggatg atgaagggca cgcccttaac gacttgacga
     2161 cagcgcgttt tgggctacgc cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat
     2221 gtacatggaa catccttttg ccgcttcaga aatctctgga tcatgctcgc atgttgcgca
     2281 atctactcgc ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg gttcacctgc
     2341 aaacggcgca tttttagaat aatcctgacc ttgtgcggaa gaaaaaacat gaaaattcgc
     2401 gccttattgg tagcaatgag cgtggcaacg gtactgactg gttgccagaa tatggactcc
     2461 aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgcgcag
     2521 gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca
     2581 gccaatagcg aatacgctaa acgtctgaca actattgcca atgcgctagg caacaatatc
     2641 aacggtcagc cggtgaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg
     2701 gctaacggct gtatccgcgt ctatagcggg ctgatggata tgatgacgga taacgaagtc
     2761 gaagcggtga tcggtcacga aatggggcac gtggcgttag gccatgtgaa aaaaggaatg
     2821 caggtggcac ttggtacaaa tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc
     2881 ggaagtttat ctcaatcaca acttggtgat ctgggcgaga aattagtcaa ttcgcaattc
     2941 tcccagcgcc aggaagcaga agccgatgat tattcttacg atcttctgcg ccaacgcggc
     3001 atcagcccgg caggtcttgc caccagcttt gaaaaactgg caaaactgga agaaggtcgc
     3061 caaagctcaa tgtttgacga ccatcctgca tccgccgaac gcgcccagca tattcgcgat
     3121 cgtatgagcg cggatggggt taagtaagtc agacataccg atctacaaca taattgtagg
     3181 tcggtaacgc ctgaatcccc gatcagagta gtgatttcac tttatttagc aaaacggcta
     3241 catcatccgg tcgggtatat cccgtatcag ggtctataat cgagctgtaa acatgtggca
     3301 taatgcgcgg cacccccgcc tccagacaag ttttcagaat cacactgaag ttatccaaat
     3361 caatcccgcc agtcggctca atcagcgtca taccattgcg cgccgccgtg gtcgccagcg
     3421 cgtatagctc cggaagcgac ctctcgccgc ccatcggaaa gaacttcgcc gcgtgtgcgc
     3481 ccatatctag catcattcgc accgccgtat cgcaggagac gcgggcaggc gtaccctggc
     3541 tgctgctaac gccggtggaa atcagcactt cgcccggcgt ccccgtcggg ctaaccagcg
     3601 cgttgacatg ggtctgctca ccgcccgtag ccgcaagcgc gcctgccgcg aaaccacaac
     3661 cggtaaaggt ctggttaacg tgtgctggat gaatctgcgc cgcaatcatc gcagctttgt
     3721 aatactgcgc cgggtcaccc gcccccagac ctactgaaat ggacggtatt tccgccatcc
     3781 aacgtttaac ttcctggatc ccctcatcca ccgaggagaa ctgcgcggaa agcacgccaa
     3841 tcactgcgtg gccttcggcg gcgtcaaaga tttcacgggc attggcgata tctttcgcca
     3901 gcacattaat tgccacgcga ttacgataga aattaatctt ttgcatgatc tgcaatctcc
     3961 ttcagtcgcg ccacgataag tgccatttcc ccttccgcca ccgtacgcgg atcaaggcta
     4021 aaaacgccct ggtggaggtt ataccgccgt gcataaatcg cgatatcgcc accacgcagt
     4081 tgcgcttcga cgacgcgagc gtcaactccc aattcctgcg catttactcg aatacgaata
     4141 cgccagattg cacgcccggc ttcatcctgc tcaatatcag ccgttaaccc gtgaatcgca
     4201 gaaatcgctt caaccactgg ctggagctgt tccgcggtga tgactgcctg cccctggtga
     4261 taattctcca gcgcgtaaac cagcccaacc atgttctcct tgccgatttt catcgccctg
     4321 gcaatgccgt gatgctgcgc tttgcaggct gcaatccaga tttttttacc agtgataaac
     4381 ccggaggtcg gcgcgttaaa ggcttttgcg ccgctgtaga ccaccatatc cgctccgctc
     4441 accacccagg cacgtaaatc ctcttcggcg gcggcatcga caatcagcgg caggttgtac
     4501 atctgcgcga catgtgcgaa gtcctcgatg ttcaacattc ccttctgcac gcagtgatgc
     4561 gacttcacat acagcagcgc ggcggtcgcg tcggtaatgg cactctccag ctgccagaga
     4621 gccgccaggt tacttgaacc cacctccaca acgcgcccgc cacctaagcg gatcgcgctg
     4681 aggatcgatg caccgtaatc gacgttatgg ccgcgcagca tcaccacttc attggccatg
     4741 ccggagctat ccggcatgag cgccacccgg gccttgtcgc cgcgtgtaat ggcggcggcg
     4801 acagcgatgg cgatccctgc tgaggcacag gaggtaacgt agctatcctc cgccccggta
     4861 aagcgagaga ccagctcgcc ggttttgtcc accagctggt caatctccac gaaagaggat
     4921 gccgcgcgcg cggtggcctg catcacctcc ggcgcgacgc tggagacgcc gagaatcgtc
     4981 atcttgccgc aggcgttaat cacctgtttc agtcccagtt gttgatagat attctgcgtc
     5041 atggcttaca gcactcccag cagcgagcag accacgctca acgccacaat cgacagcaga
     5101 atcgtggtgt aacgcggacc ctttttcacc aaatagaagt agatggcgaa cactgccgcc
     5161 agcggcagca ggccgggcgc gatggagtcc agaatctgct gtaccaccac ttcagagcct
     5221 ttcagtgcgc taatttttaa aggtgaggtt atcttgacaa agctcgcgga tagcgcgccc
     5281 atcatgataa gccccagtac gttagcgccg tagataagct ccttgatgcg ccctccctgc
     5341 agcagactga taatcgaatc gcgccccagc gtatagccct tgtgcaccat gccgtagctg
     5401 attgccagcg taatcgccgg gtagagaatc agcggcaaaa taccgcccat cgcgctgcca
     5461 ttggcggcga acgggataaa aatagcaatc aacagcggca tcaccgccgc ccagatgatg
     5521 gagtcgccca tcccggccag cggccccatc aggccggttt taatcccggt gatagaggca
     5581 tcgttaatcg cctcgccgcg ggtcttttgc tcttccatgg cgatggagat cccctggatc
     5641 accgcgccaa acgtctgctc tgagttgaag aagttcagat ggcgttttaa cgcctccacc
     5701 tgctcctcct tttgcggata gagctttttg ataatcggcg tcatcgaggc gcagaaaatc
     5761 aggctctgta aacgctcata ggagctggaa acttccgcgc ccagccagta gataaaccac
     5821 gctttggtga tatccgcttt ggtcagcgcc ccggactccc gcgcgcgctc aaccagttca
     5881 tgctgcatta cgtcgtcgct cattatgccg ctccttcatt tttcgccagg cctttaatca
     5941 ggaaggccac gcaggtgccg aaaatcgcca tcgccatgat gtccaccttg aggtacagca
     6001 ccgcgaagaa tccgccgata aaccacggca gcaggctctt tttaccaatc accataatgg
     6061 tgatggcgaa gcccagcgcc gggagaatcc cgcccatgat ttcaaacgag tgggtcagcc
     6121 agtgcggcat cagcttgagg aaactttcca ctacgttctg gccgaagtag ttggcggcga
     6181 acaccactgg gaaacgtagt accagcccca gcagcgccgg gtagagaaag gcgcagcgca
     6241 taatggctga catattggcc gtttccgcgt gtttatcggc catatgcacc caggccgcat
     6301 ttagcgtgcg acgcaactgg tcgaggaata cgccgataac gccaaatgga atcgccagtg
     6361 caatcgccag gttcgggtcc atattggcct taacggcgac aggaatggcg atacaggccg
     6421 ccagcgccgg gtctgacggc acattgccgc ctggcgttga ggtcacaccc agatagacca
     6481 actgcatccc cgcgccgata atcatcgccg caggcatatt gccgagcagc aggccgacaa
     6541 agaccgccac caccactggc tgtaacagca tggcggagaa ggtatagcca aggcgcaacc
     6601 gggcaaacca gtaatacagc cccatcaggc ttgcaaaaag taaggtatcc ataatttgtt
     6661 atcccatgtt cagagggtca gaattttttc aggatatcgt ccagcgactg cggtttatct
     6721 tccgggatgg tctggaaaac agccttaatg ccgcgacttt tcaaatcgtt aagaatgcca
     6781 acgtcttttt catccagcgt tatgttctgg aacaccgctt tacggtttgg cccgccgcct
     6841 aaaccgccaa cctgaatatc agccacgtca aagccctgct gcaccgcttc ctgaatcgcc
     6901 gccagcgacg ggaacagcac cagcacgttg ccgtcgccca gctggttctc tttccacgcg
     6961 gccgcgaagc tctggttgct aaagcagtcc actttgatat tcggcggcgc ggccatcagg
     7021 tagatatttt tcataaacgg atcggcgtcc agctcgtcgc tgaccaccgc aatacggttg
     7081 gcctgagact gccctaccca cttcgtcacc acctgtccgt ggataagacg gctgtcaatg
     7141 cgacataaca cgatgtttgc catacgattt tccttactgt tgtaattgac gaatatggga
     7201 gacgacgtcc aggcaactgt tgcgcccagc ttccaccaga tcggccacgc aggcggtcag
     7261 tgggccatgt tcgcgcttgt ccagcgcctc cagcagcagc gaggcattaa gcccggagat
     7321 caccgccacc ggataatcgg cgctcagccg cgccgccacg ttagaggtgg tgccgccgat
     7381 aaaatcggtc acgataagcg aaccttccgg catggttttc accacggctt cgacgcgctg
     7441 gtaaaactcg cccagcgtat cgacgggcat cagcgcgatt tccgtcacgc cttcgatttc
     7501 gcccatcacc atgcgcagac tgttgcagag ctgcagcccc cagccgccgt gagttagcag
     7561 cagaatttca ggtaacggat gaataggtac agttgtggtc aaaatagcct cctttgctcc
     7621 agtgtttaca tttcatcagg agcaaaggac gtgccagaat ttgtgtcacg cacagaggaa
     7681 atgagattgg atggggcgtt aagccccgta aaccagggga tcagctgtag agcagttcgt
     7741 agatataaaa atattctgcg tccgataaac ggatagcgta agcctcttca atcggcaaaa
     7801 aggaggattt gatgacgcta aaggcacgag cgtccagatc aagccggttt tctagcgcca
     7861 tctgtaacgg tttgcggtta atcacgatac gctcgaccat gcagcagcag tggatcagaa
     7921 aacgtagtgt cacctggcgg ctcggtttca gggagagcgt gttggttaag tagttcagaa
     7981 catcctcgat ctctttcaga attcgctgcg ggttgagcac cgaaatatgg ttgatgatgc
     8041 tctccatggt cagcgcgctg ataaagcgca tggcgctgcg ctccatctcc agtcggcgct
     8101 cgctgcttga gagatccggc gtcagcaggc tgagcaccag ctccggccct tgctcggaaa
     8161 acagttcctc cagcgagata aacggtatat ccggcagtcc cggctggaag gtgccgacga
     8221 tccctgccag ccgttcactg gcattaagcg cctgctgaat acgctccagg ctgcgcacct
     8281 cgttatagtc gagaatcacc atacgcgtgt cctgcgacat cagctcgcca aagctctcct
     8341 ccagcacctt tttgatcttt tccgccgtcc ccattccggt tatacatgag ataaccagca
     8401 ctttgccacc gctctcttgc tgcggcgtgc agagccgaca agggatattt ttactctgca
     8461 taagcacagc caattgcgat agatcgacag tttcgtagct taaatccagc ccaacctcca
     8521 gcaggctggt cagagtgata tttggcaaca acagaacgtc aatctggaac agcttgctga
     8581 tggtgctgcc aaaatggacc agcgatccga tatccaccat cagtatcagc cgctgccagc
     8641 accggtcctg aatcatccgg gtcagggttt ccagggtgtc atgcaccgac tgctcaaatg
     8701 gcatatcaat tgcgtagaac agatcgcgct ccagcacccg gttcacatat tgcgcctggc
     8761 tcgttgcggt ggtcgcaccg tgagcgatta gaatcacgcc gcaatccggg ctggtgtcga
     8821 tgcgctggcg gtagtggcga cactctttca ggaacaggca cagccagacc acctccgtcg
     8881 ccgggcactg aatatgcaac aactcattga ttttgcggca cagaagcgtg gcattgtcat
     8941 actcatcttt acagcgatcc agaatcaggc tggatgaata gagttgaggt ataagccctc
     9001 gccgcacata gccaatcagc gcaagaaagt gtttacgtaa cgggttcgcc aggttttctg
     9061 gcaatgtaaa tcctaatacc tgctccacgt aaccgatcag cagcgtcaca cgctcctcaa
     9121 tttgatcgcc atagcgcggt ggatgcgccg cactatggcg gctataaaga ccatattcaa
     9181 aaatcgaact gagtttgttt ttcaggatag ccagcgtttc tgcgggcggc acgttactgt
     9241 tgcgcagatt gacatattca cgagtcagga aactgtaaaa caggtcgctc tcttctattt
     9301 ccgcgctggt cgccaacgaa ttttttaacg cgggcaacgt acgcgcatcc acgttcagac
     9361 gatctttacc gccaaacaac gcatcgacca gcagacgctg ctctggcgtg gtattaaacg
     9421 acatctccgc cagtcgttta tctaactcta gcgtatcatt acgctcggtc attcccgacg
     9481 cccacgcctg cgcgcacaag aactgaatat cacttttaag ctggccgata ttgccttcca
     9541 ggggtttact taacaaccaa tgcaagagcg ttttatcaag gcttatggta cgttcaattt
     9601 tacgactttc acgctgaaag aagcttacaa tcaattcgag ctgctcctcg accgaacgct
     9661 ggcgaatacc cggtaagtca atacatacct ggatacgccg ctggaaggta cgcaatagtg
     9721 ccgagttgac cgactctgta gtggcgcaaa taaggcgcac cgaaattgaa tatgttggcc
     9781 cacttgaacc aagtgggcgg tattcacctt tatcgagaat agaaaagagc ttctcttgtc
     9841 cttcgtacgg gaggcgatgc acttcatcca gaagtaaata acctccgtca gcttgctcca
     9901 ccaatcccgt tttattctca cttgccccgg taaatgcccc cttatggtga ccaaacagat
     9961 gtgaggaaag caactccgga ttatgagcat attcggcaca gttaaagtaa accaacggag
    10021 gtggcgaacc tatggtgtgc ttgcaagcga agcaatgcat taactcagca aagaatgttt
    10081 tgccaacacc agatgcaccg gttaacagca catgtagtcc gtggggataa agcaccgctg
    10141 cgcggccttt ttctaccgca tcacggagac tacggtcgta tccgataagt ccggaaaacg
    10201 gatcatcatc tgtatatttt tctttctttg gcaataaact agccactgaa tgaactatgc
    10261 attcgcaatc atccagtttt cgattaatga gcaattctag tgcgtgacga tgtagaaaaa
    10321 aaacaggacg tccctgactt tttaccacca ggccttcatt ccataattga ttaagatctt
    10381 tactaaccga gttgcgtgcc agcccaaggt taaaaccaat cgcctcagca gtaaatgctt
    10441 tctccttcgc taaatcagac agttccagcc catacgtcag ccgctccagc tccttgtgaa
    10501 taacatcaat ccgtctcatt tctttaccgc ttaaaaaaca atagttgttc agaacataca
    10561 ctatttgtaa acgtaagaat gagaactatc gggaagaatt aatgttcctt gtggcaatct
    10621 ggtttctgaa tattcgtgtt ttaaatatta cttatggtct gaaacaacca ggcaggtgaa
    10681 cagagctaag aaaaagtagc gtagaaatat aaaaacatat atcgcactga tgaatgtttc
    10741 ccggcatgac tatgcacacc gggaaatatt tagtaaatta ctcgcccttt ttcgccgcct
    10801 gaatatacag catctccagc gccagcgtcg ccgcagccag agcggtgatt tccgactgat
    10861 catatgccgg agccacttcc actacgtcca tcccaacgat gttgagatct ttcaggccgc
    10921 gtaccagttt aatggcgcga tcggaggtca gaccgccaat taccggcgta ccggttcccg
    10981 gcgcaaatgc aggatccagg cagtcgatat caaaggtcag gtaaaccggc atatcaccca
    11041 caatttgttt cacctgggcg ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt
    11101 ccagcacggt aaagccgttg tctttatcaa attcggtacg aataccaatc tgcacggaat
    11161 gattcggatc gatcagacct tctttcggcg cggtatagaa catggtgccg tggtcaaatt
    11221 cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg taccagcgcc attttgccga
    11281 aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga
    11341 gcatacgctt accggcagcc agcagcttct cggcgtgcgc ctgcagcttc tcgctcatct
    11401 cacgggcatc gccgaaagca tataccagat caccgcagtc cacgacgttc agacgctcac
    11461 gcatgtcgaa attccacggg aagcggttgt gttcccaggc cagattcgtc gaaacctgac
    11521 ggatagctgc cggaccgtgg cgtccccccg cacgaccaga agtggccata tcgaacggca
    11581 caccagtaat cacccagtca gcatcgctgt catacggctg gaagttcatc ggcaggcgta
    11641 aaaaaccaaa ggcattggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg
    11701 atggactcct attacaaagt cgatataaaa aaacccttcc gcgtcgttag gcccgacgag
    11761 gaagggttgg atttgcgaaa cacaagtttg tgctaattat cgccttaaat gggcgaggca
    11821 tcaagcgagg ttgctatttc cgacacggtg gcggcgcata gccattgtta tctgctggaa
    11881 aagcttgtcc agagcacggt ggcagcgcgt tttgcgccgg gccttgtaat tcaaaggcgg
    11941 caaagccgat taccccggta ttacgcacat cgccttgcgc cgaattggct gcgtaggaat
    12001 caacaacgtt ggcgaactga aaagccgctt cggtatgttt atctttgcgg aatcctttaa
    12061 tcgctaacga atctccggca ttcacaatat agccgttgtt attcagcgaa ccttgcttgc
    12121 cgttcagcac atccaggcca tcaaccgtag caacgatctc gtagttggtg ttacgactgt
    12181 aattacgaac atataattga taattcattc cgactctggc gtggacaata tagcgtggtt
    12241 cattattgga tgaatcataa acacgcgtaa taggtagaga attaaaatta ccatcgcgaa
    12301 tagcatattc cagttcgtta atacgaatgc tataaacgcg gtcatagccg gaaggaacat
    12361 tagtcgaata actaataacg atcatctctg caggatcgcg ccaggctcgt tcagcagata
    12421 cgccctggac cgatgagtgc acatccccac cccaggcggt gccgacattg cccgatgcac
    12481 tgctattata ctgcgcatta ctggaacaac cggccagcag cacgattaat gccaccaacg
    12541 cgctacgtac tttccatttt ttcataaccg ttccctctgc atttaattaa tgca
//
LOCUS       DAZFAH010000140        12494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFAH010000140 DAZFAH010000000
VERSION     DAZFAH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.140"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..864)
                     /gene="uxuR"
                     /locus_tag="ACHJWB_003491"
     CDS             complement(91..864)
                     /gene="uxuR"
                     /locus_tag="ACHJWB_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4315065.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1079..2539)
                     /gene="uxuB"
                     /locus_tag="ACHJWB_003492"
     CDS             complement(1079..2539)
                     /gene="uxuB"
                     /locus_tag="ACHJWB_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4315066.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2620..3804)
                     /gene="uxuA"
                     /locus_tag="ACHJWB_003493"
     CDS             complement(2620..3804)
                     /gene="uxuA"
                     /locus_tag="ACHJWB_003493"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4315067.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4144..5487
                     /gene="gntP"
                     /locus_tag="ACHJWB_003494"
     CDS             4144..5487
                     /gene="gntP"
                     /locus_tag="ACHJWB_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO4315068.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIDNIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5730..6632)
                     /gene="fimH"
                     /locus_tag="ACHJWB_003495"
     CDS             complement(5730..6632)
                     /gene="fimH"
                     /locus_tag="ACHJWB_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4315069.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6652..7095)
                     /locus_tag="ACHJWB_003496"
     CDS             complement(6652..7095)
                     /locus_tag="ACHJWB_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315070.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDASQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(7168..7698)
                     /gene="fimF"
                     /locus_tag="ACHJWB_003497"
     CDS             complement(7168..7698)
                     /gene="fimF"
                     /locus_tag="ACHJWB_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4315071.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7708..10344)
                     /gene="fimD"
                     /locus_tag="ACHJWB_003498"
     CDS             complement(7708..10344)
                     /gene="fimD"
                     /locus_tag="ACHJWB_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4315072.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHHLAGFFVRLFVACAFVAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLTSMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLRSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRILYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVMLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10411..11136)
                     /gene="fimC"
                     /locus_tag="ACHJWB_003499"
     CDS             complement(10411..11136)
                     /gene="fimC"
                     /locus_tag="ACHJWB_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4315073.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11173..11712)
                     /gene="fimI"
                     /locus_tag="ACHJWB_003500"
     CDS             complement(11173..11712)
                     /gene="fimI"
                     /locus_tag="ACHJWB_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315074.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGVANAQAWFSLTYQ"
     gene            complement(11777..12325)
                     /gene="fimA"
                     /locus_tag="ACHJWB_003501"
     CDS             complement(11777..12325)
                     /gene="fimA"
                     /locus_tag="ACHJWB_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4315075.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2953 a         3306 c         3067 g         3168 t
ORIGIN      
        1 ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg
       61 aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca gcggccatga
      121 atcaaacaga tagccatcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg
      181 cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat
      241 taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc
      301 cagatggctg tgcaactgga tccacattgg attgttttcc cgccactgcc aggactgacg
      361 gaacagctcc accagcatgc tgttatgcgt tgcttcggca atagcgagat ggaactgcat
      421 gtcaccgctt tcgctgctgc ccggcgcact ggaagccagt tcacgctctt ccagttgcaa
      481 tgcctgacgc attttgacga tatcttcgcg ggtagcctgc aaagcggcaa actcggcgat
      541 gttgctctcc aataactgcc gcgcctgtaa cagctcaaaa ggaccggcat cgttgcagac
      601 gttggcatcc ggactgtctg tgttctggct gcctgagtta tcaagaacat agataccggc
      661 accccggcgt acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac
      721 cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg
      781 gttgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct
      841 ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt
      901 tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt
      961 aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa
     1021 aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt
     1081 acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca
     1141 ctgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca
     1201 gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt gatcttctgg aactccgcca
     1261 gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca
     1321 tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac
     1381 gcaccgggtc cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg
     1441 tacggtgacg cagagacggg ttgctgaaac gctcgatcag caacgtcgca taggcgttca
     1501 ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc agggagaagg
     1561 ccgctttgcg ataagccggg ttagtcatgg tgtcggcaat ggtttcatag ccgccgaggt
     1621 aaccgaggta cgccagaaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga
     1681 acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccggg cgaccattaa
     1741 cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt
     1801 aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc
     1861 ggtcaaccat ggtgcacggg aaggtgacat tttcttcaat ccatgccgcc agctgcgggt
     1921 cacgcgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt
     1981 tatcgcagga catcaccgta aacgctttca accctttttc acgacgcaga cgcaaggctt
     2041 cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg tgcttgatca
     2101 gcgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga
     2161 ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc
     2221 catcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc tcggtgctct
     2281 ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca
     2341 cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt
     2401 ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg caaccgagat
     2461 gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc ggcagattgc
     2521 tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcaaagagtg acggcaagga
     2581 agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg
     2641 ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga
     2701 gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg
     2761 catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc
     2821 tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt
     2881 cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg
     2941 cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt
     3001 aaaaccgttt gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga
     3061 aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa cagccatacg
     3121 cacgccgact tcttcagcaa ctggaataat cgctttcagg aagacggcaa agttttcgcg
     3181 cagcttcgct ttgtcgatat ctttgtacag ctccaggtgt ttacggaact ggtcgagggt
     3241 gtagccttct tccgcgcccg gaagaccagc aatgatgtta cgggtcagac gcgctttgtc
     3301 ttcgtcgctc atagtggcga agcgaacagc agcctgagca atttcttctt cggtgtaatc
     3361 cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc
     3421 gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc
     3481 gagcaccggc atgaagttgt agcacacggt gcgaatgcca cactgcgcca ggttgcgcag
     3541 agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc
     3601 gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt
     3661 gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt
     3721 aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat cgtttgggcc
     3781 gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa
     3841 agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg
     3901 gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat gtcaacccga
     3961 attctgaaat tggttaacca catcacaaga atttcactct cctggttgtt gctgcaaatt
     4021 gcagcaaaag gttatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt
     4081 tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaagt aaggtaaaac
     4141 gttatgcatg tgcttaacat tctctgggta gtattcggta tcggcctgat gctggtactg
     4201 aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct gtccgtcggg
     4261 atgctggcgg gcatggattt gatgtcgctg ctgcacacta tgaaagcggg cttcggcaac
     4321 acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa attgatggtc
     4381 gactccggcg cggctcacca gatagcgcat acgctgctgg cgcgtctcgg tctgcgctat
     4441 gtacagctgt cggtgattat catcggcctg atttttggtc tggcgatgtt ctatgaagtg
     4501 gcctttatca tgttagcgcc gctggttatt gttattgccg ccgaagctaa aattccgttc
     4561 ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact gttcccaccg
     4621 cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg atatggggat ggtttatatc
     4681 tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct gccgaagttc
     4741 ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc ggtagatatg
     4801 aataatctgc cctctttcgg cgtttcgatt ctggtaccgc tgatcccggc gatcattatg
     4861 atctctacca ccatcgacaa tatctggctg gtaaaagata cccctgcctg ggaagtggtt
     4921 aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc attcgtactc
     4981 tttggcaccg cacgtggtca tgacatgcag tgggtgatga acgcttttga aagcgcggtg
     5041 aagagtattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa gcagaccatc
     5101 atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa tatctcgccc
     5161 tacatcatgg catggctgat cactgtgcta attcgtctgg cgacgggtca gggtgtcgtt
     5221 tcggcgatga ccgccgccgg gattatcagt gctgcaatcc tagatccagc aactggtcag
     5281 ctggttggcg tgaatccggc gctgctggta ctggcgacgg ctgcgggttc caacaccctc
     5341 acccacatta acgatgcatc tttctggttg ttcaaaggtt actttgacct gtcagtaaaa
     5401 gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg gctgattatt
     5461 gtgctgatta ttagcatggt agcgtaaaag gacaccagag cctgccaatg gcaggctcag
     5521 actgatgaca aagtctaaaa atgtgcccgg acagtcccct cgcccctccg gggagagggt
     5581 tagggtgagg ggaacagacc agcaaagtgc gctttgttta tgccagatgc gacgctgacg
     5641 cgtcttatct ggcctacaaa gggctaacgt gcaggttttt agcttcaggt aatattgcgt
     5701 accagcatta gcaatgtcct ctgatttctt tattgataaa caaaagtcac gccaataatc
     5761 gattgcacat tccctgcagt cacctgccct ccggtacgtg cataatttgc cgttaatccc
     5821 agactcaccg ccgaagtccc tactgctcct aacgataccg tgttattcgc tggaataatc
     5881 gtaccgttgc gcgtcaactg tacgccgacg ccctgtgcag gtgaaaacga cgcggtattg
     5941 gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga ggtaataccc caggttttgg
     6001 cttttcgcac aataaacggt aagaggaatt ggcactgaac cagggtagtc cggcagagta
     6061 acggtgacat cacgagcaga aacatcgcag ccgccagtag gcaccaccac atcattattg
     6121 gcgtaaatat tccacacaaa ctggaaatca tcgctgttat agttgttggt ctgtcgcaaa
     6181 ataagcacgg caattaatga gccagcttta atcgccaccc cgcccgcact gctcacaggc
     6241 gtcaaataaa gcgccaccgg ccacggctta tccgttctcg aattataaac aacgcgcggc
     6301 gtttcgctgg tggtaggaaa tggatagcta ctgccactat attttacggt cccggaaaaa
     6361 ttagataaca cgccgccata agccgagcct cgttgcagtg tgacatagtc tgtaatggtt
     6421 tccggataat cgttatggca aaagatttgc gtcgaaagat ccacgaccag gttttgcccc
     6481 acattcacgg cgggcgcaag gtttacataa acattggcgc tgccaccgcc aatagggata
     6541 gcggtaccat tggcggtttt acaggcgaat gaccaggcat ttaccgacca gcccatcagc
     6601 agtacagcaa acagggtaat aactcgtttc attacaatca tctcttcggg ttcagctgta
     6661 ggtataggtg atgctaatca ctgcctgaat ggttccctga gtggctccgc catttactgt
     6721 caatgctctg acctgtaacg ggaagtgcgc tgattgtgag gcatcatcca cctgaactgt
     6781 tttggttgcg ccagtattca atgtgttgcc actgtcatcc tgtagctcta actggatgtt
     6841 ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg gctgccccgc tgaagctggc
     6901 agtgaccctc gacgttccca ccggacaatt agtcaactca agcgcaacat catgccaggc
     6961 cgatgccgcc ccggcagaca taagactgaa agaataaaga tcgccgagat caaccgtggc
     7021 attggtggtg gaaaccgtac acggtttggc gacgacctta ccgttcaccg tgatggtgac
     7081 atcggctgcc tgtatcgtcg cacttgcgag cgccaatatt gccgccaata catacccacg
     7141 tttgcaccat ttcatgagca tctccagtta ctgatattca agagtgaagg tagccgtggc
     7201 attgatatgc cccgcagtga caggcacctg tgtcgccatt agccgggcgt aaaaattcag
     7261 cgtatttggt ttacccggcg tcagggtcgt ccacgaaagc gcggacgatg gagcattaag
     7321 gggtatttga ttttgctgct cattcagaag ctgtattccc agtcccgaag ccgctgacac
     7381 cgtattttca agtgcaagca ggttggcatt gtggctatct gcaacgccag taaacccaac
     7441 ctttacggca gaaacggcat taccacaggg tgacagcaaa atacgaaatg gaacaacagg
     7501 agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat caacagtaaa
     7561 attggttgat tcagcggcca cactacagcc gttatccctg acatagccgc ggatagtaat
     7621 cgtgctatcc gcagccaaag cgtgactcac cgccagccac aaaaaagcgc acagaagata
     7681 aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct gggttaataa
     7741 ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat tttcctcttc
     7801 tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt
     7861 atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc caaacggcag
     7921 cggcttatta ttgtgggtca gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc
     7981 tgctcgcacg atcgccccac gagtgggaac aacgttagca accgcgttat ctaaatcgac
     8041 gttatcagcc agggtattgg tatccagcgc cactctattt tcccgatatt cagtggcata
     8101 aggcagcacg gcataaccac gccagtcggt acgcaccccc gtctggtttt cgacttttgc
     8161 atcttttgcg ccaggcgctt taacaagcac cactgtttcg tttaacggct gccccagcat
     8221 tacgccattg gcatgagcca ataccccacc gctgactccg taatagagct gcttaatatc
     8281 atcgctatgg ctgtaaccga tattggcatt gccgtaacca ccgcgataat tcagcgtggc
     8341 gtagcctgta cttccgctat ttccatcgcc tcccccggca tagccggttt gcacgctata
     8401 gctgaggttg ttgtcttcca gcaacgtacc gtatacacca gccagattgg tcatccgacc
     8461 gttgagatcg tgtgacatgc tgtagctggc actggcatgt cgccactgag atttactgtc
     8521 agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct gatcccgtcc
     8581 tttttgccag gcgtttttcg tcaggctata gctgagcgtc cagttgatat cttcgaatgc
     8641 agtatttaaa ccagcctgga attgctcatc gacattactc gttccccaat aagtttgatg
     8701 gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg tgagctgtaa
     8761 tttcccgcgt ttgttataag cgaggttgta atagtcggtg aatttcggct taacctgaat
     8821 aactccgtcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg tatcagcgaa
     8881 attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg tgcctgactc
     8941 attgagcgat ttgttataga gaatacgcac cgattgtccg tcatgctgac tgtcatcggg
     9001 aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgccc ccatgttttt
     9061 cccgataccg aaattaaaag cacgataacg atccgccagt tgcgttccac catatattgt
     9121 ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcggggtt tttcctgctg
     9181 cgcatttcca ctacggtatt ctcctgccgt aatggaataa cgagtatgcc cttcacgttg
     9241 caaaagcggg actgacgaat agggtacggt aaaaatctgc gtgctgccgt cagcctcttt
     9301 gatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga tggtaaaagg
     9361 ccccggtggc accgtactat tataaatgtc atacccattt tgtttaatag tgacctgtgc
     9421 agtaccacga gcaataccgt ggatcaccgg ggcaaatcct ctttggctat cgggtaacat
     9481 attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg
     9541 agtataacca tcacccagcg tcagccggga acgtaacggt attatgtctc gctcaagcca
     9601 ggtattgata tgctgccatt tatttttgct acctgatgat ctgtcgctac tgttataact
     9661 ccaggtggta ttgtcgcgta aacgccacgc accaatattt aacccactcc gtaggtttaa
     9721 atatgcataa tggctgttac ccccaatccg attctgtaca ctatttccgc tgaaattata
     9781 attgagcaat ccggcattaa taccgggatc ccataactca ggaggaatat aaccacgcgc
     9841 gcgattactc ataaatgcct gagggatcgt caggttgagt cgctgctgac caacatctaa
     9901 atgcgcagta gcgtcctgga ccattgtggt taatggcaca caggcatcat ccgcgaggag
     9961 attcataccg gcgacagaag ccatattcag ccccatactg gtgagttgcg cgcgtgtcag
    10021 gcagggaaca atcccttgtt cactgtcgcc cgtattaaat gtgacatcac gcgttgccat
    10081 ataaccatta ttcaaataga tatcgacgcg atacgtccct ggcggtaatt cttgcccatt
    10141 ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc gctaaaaagc gcgggttaaa
    10201 atagagttcg gcagatgaca aaggtgcctg tgcgacaaaa gcacaggcaa caaagagccg
    10261 gacaaaaaaa ccagccaaat gatgcttacg aatatgcaag cattgtgtgt ttcgctggta
    10321 aagtcttaaa ttcagatatg acattattcc tccggtttaa aatcacccgg cagtcaattc
    10381 tttttattca ggcgaaaaat tccccctgcg ttattccatt acgcccgtca ttttgggggt
    10441 aagtgcgcca taatcattta ttgttcggta agtaatattg cttcctgcat cagaaggtaa
    10501 tttaaccgtg ctttcgccca ttggaggcac caatgcattt tcaagaaccc gggttccggc
    10561 attcaactct gttaccgtca ggtaataggg tgtcgggtta atcagcgtca gagaattcgc
    10621 gctacgacga aatcttaatt tttctgcggc ctgatcgggt ggcaacgcta atttagccgg
    10681 gcgatagtac aatttaatgc ggctgataat tgcgagctgt agcgtattct cagtcaattt
    10741 tgatttatcc attgacggaa tcgctttaac gttcatccag aataaacttt cccggtcctg
    10801 tggcaattgg ttatttgttg catcaagaat acgcaaggta ttctcttttt ttcccttcat
    10861 cgcaaacaga ggtggcgtca cgataaaacg accatccttt acaccatcgg cattttccac
    10921 ccatgattga attaaatagg tgctattttc atcattattt gtcacggcaa gttgcacttg
    10981 tttttgccct gccggataaa ttacgcgagt cgcacctaag gccactcccg cttcggcgcg
    11041 tccggcaacc atcattgcca tgaacatcag gatacctgcc agcaagcaga atgttatttc
    11101 ctgcgatttc cttacattga cgtttttatt actcactgtc ctgttcctgt tatcacatta
    11161 tctgctgaac aattactgat aggttaaaga gaaccaggcc tgggcattgg cgacgccgcc
    11221 agtaacccga cgcccggtag cacgatattt ggcgatgaaa tgtagcgaag tagagcctga
    11281 gtaaagccgt ttccagtttg ctggaggacg attaatcggt acgaggtttc cttcatcatc
    11341 aaacaacgct acgccaatat tggtggctat ccctggcccc tctcccacgg aaagcacatc
    11401 cggattttta ccatccgcga caccgtgaaa cgccacaccc acacgttcac tcaccaccgt
    11461 gctacattcc cgtaaatgaa taacaaaagg caccggtgcg ctatcttccc ctaccgcatg
    11521 aaaccggtta ctgctgattt gccccatatt gaccgtcatt tgtttatcac cggcttcaat
    11581 ccggcaagtt tccgcaataa tgacgccctg aaattgcata tttccgccgg gcagcgtggt
    11641 attccattta tttccggcca gagcaaacat tggcagcaac gacgctaata gaaataatct
    11701 cttccttttc atcgttattt ttatcgcaca agggtgggca tccctgcccg taatgacgtc
    11761 cctgaacctg ggtaggttat tgatactgaa ccttgaaggt cgcatccgca ttagcagcac
    11821 ccggggttgc ggcaccggtt gcaaaataac gcgcctggaa cggaatggtg ttggttccgt
    11881 tattcagggt tgtttctgaa ctaaatgtcg caccgtccag cgccagcgca gcacccgttc
    11941 tgtccaggat ctgcacacca acgtttgttg cgctacccgc agctgaactc tgcagagcca
    12001 gaacgttggt atgacccgca tcaatcgccg tacctaaaaa ggcaacagcg gctttagatg
    12061 caacattggt atcgcaatca ttcagctgaa tgttaaaacc gacagcagaa ctggtcgctc
    12121 cttcctgtgc cagcgatgcg gtacgaacct gtcctaactg aacggtttga tcaacagagc
    12181 ctgcatcaac tgcgcaagcg gcgttaacaa cttccccttt aaagtgaacg gtcccaccat
    12241 ttaccgtcgt ggcagcggcc agagccgctg tagaactgag ggacagagcc gacagaacaa
    12301 cgattgccag agttttaatt ttcatgctgc tttcctttca aaaaactatt tctaaatcga
    12361 catgggcagt cgttctgtac actttgtttt gtcaacgagt ttgactgcca acactgcaca
    12421 gttttccccc aaaagatgaa acatttgggg ccattttgac tcatagagga aagcatcgcg
    12481 gacaaacttt ttca
//
LOCUS       DAZFAH010000141        12126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFAH010000141 DAZFAH010000000
VERSION     DAZFAH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.141"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..254)
                     /gene="dinJ"
                     /locus_tag="ACHJWB_003502"
     CDS             complement(<1..254)
                     /gene="dinJ"
                     /locus_tag="ACHJWB_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4315076.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLG"
     gene            440..1213
                     /gene="yafL"
                     /locus_tag="ACHJWB_003503"
     CDS             440..1213
                     /gene="yafL"
                     /locus_tag="ACHJWB_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4315077.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(1270..1626)
                     /locus_tag="ACHJWB_003504"
     CDS             complement(1270..1626)
                     /locus_tag="ACHJWB_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4315078.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1619..1897)
                     /locus_tag="ACHJWB_003505"
     CDS             complement(1619..1897)
                     /locus_tag="ACHJWB_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4315079.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(2002..3714)
                     /locus_tag="ACHJWB_003506"
                     /pseudo
     CDS             complement(2002..3714)
                     /locus_tag="ACHJWB_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3686..4471
                     /gene="lafU"
                     /locus_tag="ACHJWB_003507"
     CDS             3686..4471
                     /gene="lafU"
                     /locus_tag="ACHJWB_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4315080.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            4542..5597
                     /gene="dinB"
                     /locus_tag="ACHJWB_003508"
     CDS             4542..5597
                     /gene="dinB"
                     /locus_tag="ACHJWB_003508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4315081.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5594..6046
                     /gene="yafP"
                     /locus_tag="ACHJWB_003509"
     CDS             5594..6046
                     /gene="yafP"
                     /locus_tag="ACHJWB_003509"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4315082.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            6291..7430
                     /locus_tag="ACHJWB_003510"
     CDS             6291..7430
                     /locus_tag="ACHJWB_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4315083.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            7427..8041
                     /gene="prfH"
                     /locus_tag="ACHJWB_003511"
     CDS             7427..8041
                     /gene="prfH"
                     /locus_tag="ACHJWB_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4315084.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(8098..9555)
                     /gene="pepD"
                     /locus_tag="ACHJWB_003512"
     CDS             complement(8098..9555)
                     /gene="pepD"
                     /locus_tag="ACHJWB_003512"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4315085.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9816..10274
                     /gene="gpt"
                     /locus_tag="ACHJWB_003513"
     CDS             9816..10274
                     /gene="gpt"
                     /locus_tag="ACHJWB_003513"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4315086.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            10366..11610
                     /gene="frsA"
                     /locus_tag="ACHJWB_003514"
     CDS             10366..11610
                     /gene="frsA"
                     /locus_tag="ACHJWB_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4315087.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            11668..12069
                     /gene="crl"
                     /locus_tag="ACHJWB_003515"
     CDS             11668..12069
                     /gene="crl"
                     /locus_tag="ACHJWB_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4315088.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         2938 a         3050 c         3166 g         2972 t
ORIGIN      
        1 cctaatttat caaataaatc atcggcgtct ttggccttat gaacatcaac gccagcttcg
       61 ctgtttttga ttgattgaat ggttaattga ttaggctcgc gtaaatcaaa cggcaatgcc
      121 ttttcacgcg cgacctttgt gagggttatg cgaaccaggt cagagatggt cagccccatc
      181 ccggccagta cgtccgccgc ctgattcttc agatcttcat cgattcgggc gcgaacaaat
      241 gcgttagcag ccattggggt atctcctgtg ctgtatattt attcagcttg aattgtagct
      301 caaatgagca acagcgacaa tccatgagta atttaataaa cggatgtttt tacataatta
      361 actgaaatat atttattaat tttataataa tggtttatta agtttgttca ggtacgggcc
      421 gggatacact gcaacatcaa tgcatgatca gccattattg agtatgtcct tgccgtcgat
      481 tccctctttt gtgttgtcgg gattactgtt gctttgcttg ccgttttctt catttgccag
      541 cgccaccaca tcacatatca ctttcagcta cgccgcccgc cagcggatgc aaaatcgtgc
      601 gcgtttatta aaacagtacc aaacccatct gaaaaaacag gccagctata ttgtggaagg
      661 caatgccgaa agcagaaggg cgctacgcca gcacaaccgc gaacagataa aacagcatcc
      721 agaatggttt cctgcgccgc tcaaggcgag tgacagacgc tggcaggcgc tggcggaaaa
      781 caaccatttc ttaagcagcg accatctgca taacatcacc gaagtggcga ttcaccgtct
      841 ggagcaacag cttggcaagc cttacgtctg gggcggtacg cggcccgatc aaggcttcga
      901 ctgtagcggg ttagtttttt atgcctacaa caagatcctt gaggcgaaac tcccacgcac
      961 ggctaatgag atgtatcact atcgccgggc aaccattgtg gcgaataacg acctgcgccg
     1021 gggagatttg ctatttttcc atatccacag ccgcgagatt gccgaccata tgggggtgta
     1081 tctgggcgac ggtcagttta ttgagtcacc gcgcaccggg gaaaccattc gggtaagtcg
     1141 gttagctgaa cctttctggc aggatcactt tttaggcgcg cggcggattt tgacggagga
     1201 gacgattttg taaatgggta atattgacga actggcaaaa ggtcggatgc cacgcggcat
     1261 ccgacctgtt tacatctggg ctgaaaactc acgttctaag acatcaacaa tgagctggtt
     1321 ttttgaaatg gaatgttgtc tggcgacgat ggtcagccgc gaatcaatgt gatgaggaac
     1381 acgaagcgtc aggcgttttt gctcttcttc ttcacgaaaa ggggcaatac cgtcctcttc
     1441 acaatccgcc atccactcat ccagcgcgct ttcgccttca cgatataatg cctcaatact
     1501 tgtggctaca aagtcacaat aaccgtttac attgaggaac ttgccgcgaa atgcgttgat
     1561 atcggcttca aatgtcactg cggctggttg acctttaatt gtgataatgt tcgatgcttt
     1621 attcataatc tacccctaca cagttaacaa accactcctt aagacctgct actgcgcctt
     1681 tatccatcac attctttgga tgtggctcat gtgtgctgaa tttggtcgtt ccaatctgaa
     1741 attttcgtct ggagccgttg ttatttttta ttgttccacc cacagcttta atgagtgcaa
     1801 caacatcatc ccatttaatt ccagattttg gcggctccgt cattacatcg tcgaatgttt
     1861 ttctatgacg agaacctagc gcgctgacct taatcatact atcaatccat gacgtcattg
     1921 tatggtgtca tcgtagtctc tttgcagtgt gacgtcaatt aatggtgtca ggtgctgcaa
     1981 agcatggaaa agaatccccc atcaactcaa cgcccccata atcttcaact ccagctcatc
     2041 cggcacttcg ttatacgaca gcacatgcag ccccggcgca aacaaccttg cataacgcgc
     2101 cagcaaaggg cgcagctgcg gtggcaccag cagcaccggg tctttccccg ccgctttcat
     2161 ctgctctttc acctgtggca tcgtactctg gaactggttg agcatattcg ggtccactgg
     2221 cacgctgtcg agcatcactt taccgccctg ttgcgcctga ttcactaggt tagtcagcag
     2281 attttccagc tcattattca gggtatacac cgtcagctcc tgcttgcgaa cgaacggatg
     2341 ggtaatgctg cgccgcagcg ccaggcgcac atcggctgcc agcagaatat gatctttggt
     2401 caccgcgcta ctggcgacca gcacggtggc gatggtgacg atatcgcgca gggaaacgcc
     2461 ttcggtcagc agcgcccggt acactttcag caactggctg taattgagca ccgcgcttaa
     2521 atcctccgcc aggcgcggtg ccatcgacgc caaacggtta tgcaactgcg taatatcgtc
     2581 atagttaaat aaatcaggaa tatagctgcg cacaatctta tttacatggg tagcaatcac
     2641 gctggcgctg tcgatcacct gataccccat attcagcgcc ttcgctttct gcgcaggctg
     2701 gatccaggtc accggcatcc cgtacgccgg gtcgttcccc agcacgccgt ctatctcgcc
     2761 gtaggtttcg ctggagggca gcgccatcag tttatccgcc ggaatatccg cttcatcagc
     2821 cttaatgccg ttgatgaaaa tagcgtactg gctgggctta aggcggaagt tttcccgaat
     2881 gcggatctcc ggcagcagca cgccgttgcc gtcggatatc acctgtcgca cgccgcgaat
     2941 acgctgggtg agcgggttgc cctgggcttt gtccaccagc gccaccagct tgtagccgag
     3001 gcttaagctg atgggctcga tcagcggaat ggtttcccaa ctgacctgtt gctcgctggt
     3061 ttcggtgatg gtgcgggtca gcgtttcgag acttttctct tccgcttccg ccgcctgcgg
     3121 gcgtttgctc atccgccagc cggtaaagcc aagcagggcg ctgaacagca aaaacggcag
     3181 atgtggcatt ccaggcacta ccgccagtac gaacataatt ccggtagcgg tgtagagcac
     3241 cgatgggctt gccagcaact ggttacgcac gtcatgggcg atatcgccgt tgtcgctgac
     3301 gcgggtgacg ataatcgccg ccgcggtgga gagtagcagg gaagggattt gcgccaccag
     3361 cccgtcgccg atggtcatca gcacatactg ctggaaggcg gcgtcggcgc tcaggttgta
     3421 tttgaagatc ccgatacaga cgccgccgat caggttgatc gccagaatca tcatcccggc
     3481 gatagcatcc ccgcgcacaa atttcgatgc cccgtccatc gcgccgtaga aatcggcctc
     3541 gctggcaaca tctttacgtc gggtttgtgc ctgcgcctga ttgatcaacc cggcattaag
     3601 atcggcgtca atcgccatct gcttaccggg catcgcgtcc agggtaaagc gggcagaaac
     3661 ctcggagata cgctcggccc ctttggtgac gacaataaag ttgattgtca acagcgtcag
     3721 taaatccgag cgtgaaagca ttatcgccgc gctgcacggg cagtcgattt ttaacggtgg
     3781 tgggctgtcg ccgttaaata aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc
     3841 ggtgccggaa gagacggaga aaaaagcgcg cgacgtcaac gagaaaacag cgctgctgaa
     3901 gaagaaatcc gccaccgagc ttggcgagct ggcaaccagt atcaacacca ttgcccgtga
     3961 tgcgcatatg gaagcgaatc tggagatgga gattgtcccg cagggattac gcgtgctgat
     4021 taaagacgac cagaatcgca atatgttcga acgcggcagc gcgcagatta tgccgttctt
     4081 taaaacgctg ctggtggagc tcgcgccagt gttcgactcg ctcgataata aaattattat
     4141 taccgggcat accgatgcga tggcctacaa aaacaatatc tacaacaact ggaacctttc
     4201 gggcgatcgc gcgctttcag ctcgtcgggt gctggaagag gccggaatgc cggaagataa
     4261 agtgatgcag gtaagcgcaa tggcggacca gatgctgctg gacgccaaaa atccgcaaag
     4321 cgcgggcaac cggcgcattg agattatggt gctgaccaaa agtgcgtccg atacgctgta
     4381 tcaatacttt ggtcagcatg gggataaagt ggtgcagccg ctggtgcaaa agctggataa
     4441 gcagcaggtg ctttcgcagc gaatgcgcta aatgctgaat ctttacgcat ttctcaaacc
     4501 ctgaaatcac tgtatacttt accagtgttg agaggtgagc aatgcgtaaa atcattcatg
     4561 tggatatgga ctgctttttc gccgcagtag agatgcgcga caatcccgcc ctgcgcgata
     4621 tccctattgc tattggcggc agccgcgaac gtcggggggt gatcagcacc gccaattatc
     4681 ccgcgcgtaa atttggcgta cgtagcgcaa tgccgacagg gatggcgctc aaattatgcc
     4741 cacatctcac cttgcttccg gggcgctttg acgcctacaa agaagcctca aatcatatcc
     4801 gtgaaatctt ctcgcgctac acctcgcgca ttgagccgtt gtcactggat gaggcttatc
     4861 tcgatgtcac cgatagcgtc cattgccacg gttctgcgac cctcatcgcc caggaaatcc
     4921 gccagacgat tttcagcgag ctgcaactga cggcgtctgc gggcgtggca ccagtaaagt
     4981 ttctcgccaa aatcgcctcc gacatgaata aacccaacgg ccagtttgtg attacgccgg
     5041 cagaagttcc ggcattttta caaaccttac cactggcaaa aatccccggc gtcggcaaag
     5101 tctcggcggc aaaactggaa gcgatggggc tgcggacctg cggtgatgta caaaagtgtg
     5161 atctggtgat actgcttaaa cgctttggca aatttggccg cattttgtgg gagcgtagtc
     5221 aggggattga cgagcgcgac gttaacagcg aacggttgcg aaaatccgtc ggcgtggaac
     5281 gcacgatggc ggaagatatc caccactggt ctgaatgtga agcgattatc gagcggctgt
     5341 atccggaact tgaacgccgt ctggcaaagg taaaacctga tttactgatt gcccgccagg
     5401 gggtgaaatt aaagttcgac gattttcagc aaaccactca ggagcacgtc tggccgcggc
     5461 tgaataaagc tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc
     5521 gcggtgtgcg actggtgggg ctgcatgtga cgttgcttga cccgcaaatg gaaagacaac
     5581 tggtgctggg attatgaata acatacaaat aagagactat cagcctggcg attttcagca
     5641 actatgcgct attttcatta gagcggttac gatgactgcc agtcagcatt attcaccaca
     5701 acaaattgcc gcctgggcgc agattgacga atctcgctgg aaggagaaac tcgcgaaatc
     5761 acaagtgcgg gttgcagtca ttaatgcaaa actggtcggc tttattacct gcgttgaaca
     5821 ctatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg ccagcgcttt
     5881 gttaaaacct ttgattaagt ctgaatccga acttacggtg gacgcaagca taaccgcaaa
     5941 accctttttt gaacgttatg gttttcagac agttaagcag caacgcgttg aatgtcgggg
     6001 agagtggttt attaattttt atatgcgtta taaacagcaa cattaaatcc agcttgcaat
     6061 gaaaataacg caagcctagt atgtgcgcgt tttcttattc cacaaactgc aaggaggtaa
     6121 atcatgacaa atcctttatc aatgactctt tgcagacctt tccaggatta attctttttt
     6181 cttgccctgg attcgtctgc catttcctga ttttttaatt gttactctca ataattcaag
     6241 tcgcaacaca tgcaacttga agtatgacga gtctatttac ggaatggtat atgggcaatt
     6301 atattcgtcc cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga
     6361 gtttagcgat ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgtagtgg
     6421 ggatgccaga tttacatccc ggacgcggct atccgattgg cgcagcgttc ttctctgttg
     6481 gtcgttttta tccggcactg gtcggcaatg atatcggctg cggtatggcg ctatggcaaa
     6541 cagatattct cgctcgcaaa tacaacgccg ataagtttga aaagcgatta tcagatctgg
     6601 atgacgttgc tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc
     6661 cgtggcgcag ctcgcttggc tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag
     6721 ttgatcaaat catcaacgct gaactgtttg cactggcagg tctggatgca caacatctgc
     6781 aactgttggt tcatagcggc tcgcgggggg tggggcagtc tattttacag cggcatattg
     6841 cctcgttctc gcatcatggt ttgcctcaag gcagtgacga cgcgctacgg tatattgcgg
     6901 aacatgacga tgcgctggcg tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa
     6961 tgcaacaagt taaggccacg ggaagtccgg ttctggatgt ggcgcataac tttgttagcg
     7021 cgtgtcaaat cggcgatcaa cagggctggt tgcatcgtaa aggtgccacg ccggatgaca
     7081 acggtctggt gattattccc ggttcacgtg gtgattactc ctggattgta cagcctgttg
     7141 cgaacgaaaa aacgctccac tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg
     7201 agtgtaaagg gcgtctggca gcgaaataca cggcgacgca actctcgcgt actgaacttg
     7261 gcagtcgggt aatttgtcgc gataaacaac tcatctttga agaagcgcca caagcctata
     7321 aatcggctga aagcgtggtg caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc
     7381 ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca
     7441 actctcctca gcgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag
     7501 gctaattaaa gaagctgccc gacaagacgt cgcggtaacg gtgttggaaa cagaaacggg
     7561 ccgctactct gacacgctgc gttcggcgct gatttctctg gatggcgata acgcatgggc
     7621 attaagcgaa agctggtgtg gcactattca gtggatttgt ccgagtccgt atcggcctca
     7681 tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggaaca
     7741 atcggatgca atccgttatg agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa
     7801 taaaaccgac tcggcggtac gcgccacgca tttggcatcc ggtattagcg tgaaggttca
     7861 gtcagagcgt agtcagcatg ctaacaagcg gctggcacga ttgctgattg cctggaagct
     7921 ggagcagcag caacaggaaa atagcgcggt gctgaaatcg cagcggcgaa tgttccatca
     7981 ccagattgaa cgtggcaacc cgagacggac atttacaggg atggctttta tcgaaggata
     8041 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
     8101 cttcgccgga atttctttca gcagttcagt cagcagcgtc cagtaatgac ctacgctttc
     8161 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
     8221 gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg
     8281 gatgttcggt gttttgttga acagacgctg ataggtttca cgtacaagat gcatcaccgg
     8341 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
     8401 tagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagtga
     8461 acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
     8521 ggaggtttcg accacacctt ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc
     8581 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
     8641 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
     8701 ctcctgatag gtattcacca gagatttcag gacgtcgact ttatcagctg cgacagcaat
     8761 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
     8821 gcgcagatcc agttcttccg catgacccgc caggaagcgc accagtagtt tgttggcatt
     8881 acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa
     8941 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     9001 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     9061 cagaatatca gcctgcaacc agttgctctg taagccaaaa gcaccgtcca taccggcttc
     9121 ttcggtcatg gtcagcagca cttccagcgg accgtgaacc acgttttcgt cagccagaac
     9181 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
     9241 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     9301 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc
     9361 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     9421 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     9481 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     9541 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     9601 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac
     9661 cactttatcc cgcgtcaggg agaggcagtg tgtaaaaaga cgcggactca tgtgaaatac
     9721 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     9781 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     9841 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     9901 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     9961 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
    10021 gagagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcata gttattgatg
    10081 acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca aaagcgcact
    10141 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
    10201 tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
    10261 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
    10321 ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
    10381 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
    10441 cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct
    10501 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa
    10561 atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta
    10621 tacgatactg tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg
    10681 atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg
    10741 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
    10801 gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg
    10861 ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
    10921 catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg
    10981 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg
    11041 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
    11101 agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact
    11161 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatatctt
    11221 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctattgagt
    11281 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg
    11341 gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa
    11401 gtgcaaggat tgctgggacg tcgctgccca acgccaatgt tatcaggcta ctggaagaac
    11461 gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa
    11521 ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc
    11581 accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa
    11641 acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga
    11701 ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat
    11761 cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt
    11821 gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac
    11881 cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agatactgaa
    11941 gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg
    12001 acgacgctga atctgaagct ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta
    12061 acggcgtgag tgaaatgtgc cggatgcatc acatccggca atatctatta aaactgatac
    12121 gtcatc
//
LOCUS       DAZFAH010000142        11994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFAH010000142 DAZFAH010000000
VERSION     DAZFAH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.142"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            244..1014
                     /locus_tag="ACHJWB_003516"
     CDS             244..1014
                     /locus_tag="ACHJWB_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4315089.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1005..1478
                     /locus_tag="ACHJWB_003517"
     CDS             1005..1478
                     /locus_tag="ACHJWB_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4315090.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1586..2125
                     /gene="dnaT"
                     /locus_tag="ACHJWB_003518"
     CDS             1586..2125
                     /gene="dnaT"
                     /locus_tag="ACHJWB_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4315091.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNTPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2128..2865
                     /gene="dnaC"
                     /locus_tag="ACHJWB_003519"
     CDS             2128..2865
                     /gene="dnaC"
                     /locus_tag="ACHJWB_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4315092.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2914..3408
                     /gene="yjjA"
                     /locus_tag="ACHJWB_003520"
     CDS             2914..3408
                     /gene="yjjA"
                     /locus_tag="ACHJWB_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4315093.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3662..5953
                     /gene="opgB"
                     /locus_tag="ACHJWB_003521"
     CDS             3662..5953
                     /gene="opgB"
                     /locus_tag="ACHJWB_003521"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4315094.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6092..7114)
                     /gene="lgoD"
                     /locus_tag="ACHJWB_003522"
     CDS             complement(6092..7114)
                     /gene="lgoD"
                     /locus_tag="ACHJWB_003522"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4315095.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7253..8167
                     /locus_tag="ACHJWB_003523"
     CDS             7253..8167
                     /locus_tag="ACHJWB_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4315096.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8382..9743
                     /locus_tag="ACHJWB_003524"
     CDS             8382..9743
                     /locus_tag="ACHJWB_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4315097.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9792..11453)
                     /gene="tsr"
                     /locus_tag="ACHJWB_003525"
     CDS             complement(9792..11453)
                     /gene="tsr"
                     /locus_tag="ACHJWB_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4315098.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAALEEQASRLT
                     EAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11572..>11994)
                     /gene="hpaR"
                     /locus_tag="ACHJWB_003526"
     CDS             complement(11572..>11994)
                     /gene="hpaR"
                     /locus_tag="ACHJWB_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO4315099.1"
                     /translation="LLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDFHDLA
                     YRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEEAY
                     RQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2908 a         3168 c         2923 g         2995 t
ORIGIN      
        1 ccggttatct attatcctga ttcgtaaagg ccaaacaata aattcattat gatgataata
       61 ttcatgtgat agtttgtttg cggcgagaga taattcgctt tttatcaccg agcttctccc
      121 gataaccagg attaatcagc gcaattttga tcttaaccta ccgcaaaaca accgtgcttc
      181 atgatacaaa tggattctca ttatctgaac aatacgcagc acgtctatga caaagggcga
      241 gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt gtatccagtg tggattattt
      301 cttttgcaac atggtgcgga aagcgcgttg gttgatgagc tttcctcacg actgggtcgg
      361 gcgctgggaa tggacagcgt tgaaagttct atctcttcga acgccatagt gctgacaacc
      421 attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc acgatcgcgg cattaatatg
      481 catgtggtga ctgaagtcca gcacattgtg attctggcgg agcaccatct gctggattac
      541 aaaggcgtag agaaacgatt tagccaaatt cagccattac gttacccaag atggctggta
      601 gccttaatgg ttggcctttc ttgtgcctgt ttctgtaaac tcaataaagg tggctgggat
      661 ggtgccgtca tcaccttctt tgccagtacg gccgcgatgt atatccgcca gctgctggca
      721 caacgtcatc ttcatccaca aatcaacttt tgccttaccg ctttcgccgc caccaccatt
      781 tccggattgc ttttgcaact ccccactttc agcaataccc ccaccattgc gatggccgcc
      841 agcgttctgc tgctggtgcc gggatttccg ttgataaatg ccgtcgccga tatgtttaaa
      901 ggccacatca ataccggact ggcacgctgg gcgatcgcca gtctgctgac actggctacc
      961 tgcgtcggcg tggtgatggc actgacgatt tgggggctac gcggatgggt gtgatcgagt
     1021 ttctgtttgc attggcgcag gatatgatcc tcgccgccat tccagcggtc ggctttgcga
     1081 tggtgttcaa cgttcccgtt cgggcattac gctggtgtgc gctgcttggc gcgattggtc
     1141 atggttcacg aatgatctta atgaccagcg ggctgaatat tgagtggtca acctttatgg
     1201 cttctatgct ggtcggcacc attggtattc aatggtcgcg ctggtatctg gcacatccga
     1261 aagtgtttac cgtggcggct gttatcccta tgttccctgg catatcggct tataccgcaa
     1321 tgatttcggc cgtaaaaatc agccagttag gttacagcga gtcattgatg attaccctgt
     1381 taaccaattt tcttacagct tcatcgattg ttggtgcgtt atccatcggt ctttccatcc
     1441 ctggattatg gttgtaccgc aagcgccctc gcgtataaaa ttgcctcgct taaagcacgc
     1501 ggatgagaga cagccacctc tcctccgtgt gttactataa aagttatctc ccttctcgtt
     1561 catcgttcca tatttgagaa acagtatgtc ttccagagtt ttgaccccgg acgtcgttgg
     1621 tattgacgcc ctggtacacg atcaccaaac cgttctggca aaagctgaag gcggtgtggt
     1681 tgccgtattt gctaacaata ccccggcgtt ttatgccgtc acgcctgcac gcctggctga
     1741 actgctggcg ctggaagaaa agctggcgcg tccgggaagc gatgtcgctc tggacgatca
     1801 actctatcag gaaccgcaag ccgctcccgt tgctgtaccc atggggaaat tcgccatgta
     1861 tccggactgg caacccgatg ccgattttat ccgcctggcg gcgctatggg gtgtggcgct
     1921 aagagagccg gtgactaccg aagaactggc ctcattcatt gcctactggc aggcggaagg
     1981 taaagtcttt caccatgtgc agtggcaaca aaaactggcg cgcagcctgc aaatcggtcg
     2041 tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg gtcagtgaac ctgacagcca
     2101 aattccacca ggattcagag ggtaacgatg aaaaacgttg gcgacctgat gcaacgcctg
     2161 caaaaaatga tgcctgccca tatcaaacct gcattcaaaa cgggtgaaga actactggcc
     2221 tggcagaaag aacaaggagc gatccgctcc gccgctctcg aacgtgaaaa tcgggcgatg
     2281 aaaatgcagc gcacctttaa ccgctccggt attcgtccac tgcatcagaa ctgctccttt
     2341 gagaactatc gcgttgagtg tgaagggcag atgaatgcgt taagcaaagc gcgccagtat
     2401 gtcgaagagt ttgacggcaa catcgccagc ttcatctttt ctggtaagcc aggaaccggc
     2461 aaaaaccatc tggcggcggc aatctgcaac gagctgctgc tacgcggtaa atccgtattg
     2521 atcatcaccg tggccgatat tatgtcggcg atgaaagata ccttcaggaa tagcggtacc
     2581 agcgaagaac aactgcttaa cgatctgagc aacgttgatc tgctggtgat cgatgagatc
     2641 ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca accagatcgt cgatcgccgc
     2701 tcttcttcca aacgcccaac agggatgctg accaacagta atatggaaga gatgaccaag
     2761 ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta acagtttgtg ggtgatcttc
     2821 aactgggata gctaccgtag ccgtgtaaca ggtaaagagt attaatcccc gtttctggcg
     2881 gtatactgcg acacaattcc accttgagta atgatgatga aaactgttaa acatcttctg
     2941 tgctgtgcca ttgccgccag cgcattaatt tccaccgggg tgcatgctgc gtcctggaaa
     3001 gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa acagtacgac acaggaaggc
     3061 ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg gaaaccaggc cttaagcgca
     3121 gataacatga acaatgccgc aggcattctg caatactgcg cgaagcaaaa gctggcttcg
     3181 gtaaccgatg ccgaaaacat caagaaccag atgctggaaa agctgggcct gaacagtgaa
     3241 gagcaaaaag aagacaccaa ctatctggac ggcattcagg gtttgctgaa aaccaaagat
     3301 ggtcagcaac tcaatctgga taacatcgga acgactccgc tggcagaaaa ggtgaaaacc
     3361 aaagcctgcg atctggtgtt aaagcagggg ctgaacttca tttcctgaac cgcgaaacgc
     3421 cgcgttttag ctgttacccg gctgacgcgg ctctctcctg cccttttccc tgctcctgga
     3481 cggttttccc cctgttacac gggaagcgac cgcgatcaaa aaagtctgtt tataaactaa
     3541 ttcctaacaa catcgcattt ttttgacact agaattgcag caatatggcg acgccattac
     3601 attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc cggtttagtg aggatcgccc
     3661 gttgtcagaa ctactctctt tcgccctttt tctcgcctct gtgctgattt acgcatggaa
     3721 agcgggacgt aacacctggt ggtttgcagc cacattaacg gtgctggggc tatttgtcgt
     3781 tttaaatatc accctgtttg ccagcgacta ttttactggc gatggtatta acgacgcggt
     3841 tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc agcaaataca tcctgccggg
     3901 tatcggcatt gtgctggggc tgacagcggt gttcggtgcg ctgggctgga tcctgcgccg
     3961 tcgtcgccat catccgcacc attttggtta cagcctgctg gcgctcttac tggcgctggg
     4021 ttcggtggac gccagcccgg catttcgtca gataacggaa ctggtgaaat cccagtcacg
     4081 cgatggcgac ccggactttg cggcttatta taaagagccg tcgaaaacta tccctgaccc
     4141 gaaactcaac ctggtttata tctacggcga aagtctcgag cggacctatt ttgataacga
     4201 agctttcccg gatctcacgc ctgaactggg cgcgttgaaa aatgaaggcc tggatttcag
     4261 ccacacgcag cagctgccag gaacggatta cacgattgcg ggcatggtgg cttctcagtg
     4321 cggcataccg ctgtttgccc cctttgaagg caacgcctcc gcctctgtct ccagcttctt
     4381 cccgcagaac atctgtctgg gcgatatcct gaaaaactcg ggttatcaga actatttcgt
     4441 gcagggcgcg aatctgcgtt ttgccggtaa agatgtgttc ctgaagtcgc atggcttcga
     4501 ccacttatac ggctcagaag agctgaaaag cgtggtggcc gacccgcact atcgcaacga
     4561 ctggggattc tacgacgata ccgttctcga tgaagcgtgg aaaaagtttg aagagctttc
     4621 ccgctcaggt cagcgattct cactgtttac cctgacagtc gatacccatc acccggatgg
     4681 ttttatctcg cgtacctgta accgcaaaaa atatgatttt gacggtaagc cgaaccagtc
     4741 attcagcgcg gtaagttgca gccaggagaa tatcgcggcg tttatcaaca aaatcaaagc
     4801 atcaccgtgg tttaaagata ccgtcatcgt cgtctcttct gaccatttag cgatgaacaa
     4861 cacggcgtgg aaatacctta ataagcagga tcgcaataac ctgttttttg tcattcgtgg
     4921 cgacaagccg cagcaagaga cgctggcagt gaagcgtaac acgatggata acggcgcgac
     4981 ggtgctggac attctcggtg gcgataacta tctcggactt ggtcgtagca gtttatccgg
     5041 gcagtcgatg tcggaaatct tcctcaatat caaagagaaa acattggcgt ggaagccgga
     5101 tatcatccgc ctgtggaaat tccctaaaga gatgaaagag ttcactatcg accagcagaa
     5161 aaacatgatt gccttctcgg gtagccattt ccgtttgccg ctgctgttgc gggtttcaga
     5221 caagcgcgta gaaccgctgc cggaaagtga atactcagca ccgctgcgtt tccagctggc
     5281 cgatttcgct ccacgcgaca atttcgtctg ggttgaccgt tgctacaaga tggcacaact
     5341 ttgggctccg gaactggcac tctccaccga ctggtgtgtc tcgcaggggc agcttggcgg
     5401 tcagcaaatt gtccagcatg ttgataaaac aatgtggaag ggcaaaacgg catttaaaga
     5461 tacggtcatc gacatggcgc gttacaaaag caatgtcgat acgctgaaaa ttgttgataa
     5521 cgatattcgc tacaaagccg acagtttcat cttcaacgtc gccggtgcgc cagaagaggt
     5581 gaaacagttt agcgggattt cccgtccgga gtcgtggggc cgctggtcca acgctcagct
     5641 gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg aagaaatttg acctggtgat
     5701 taccgccaaa gcatacggca ataacgccag ccgtcctatt ccggtacgcg taggcaatga
     5761 agaacaaact cttgtgctgg gcaatgaagt aaccaccacc acactgcatt tcgataaccc
     5821 aaccgatgcc gacacactgg taattgtgcc gccggaacct gtctcaacca acgaagggaa
     5881 tatcctcgga cactcgccgc gtaagctcgg gatcggcatg gtggaaatta aagttgtgga
     5941 acgtgaaggg taattcctga ctgatgtgta ggtcgcataa ggtgcgacct acacaccttc
     6001 tccggcctac aaaacatcat tgagaaacag aaaaaaacag cgcccaacac ttccccggac
     6061 gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc ttaatcaact
     6121 cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac ggatagcgat
     6181 gggttaacat catgtcggca gtgattttcc cttctgccat cagtcgaccg actttagcga
     6241 aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta tggaattccg
     6301 gatcggaaaa ctgcaactca cctttaaaca ggccgacaaa taccaccgtg ccgccgtgac
     6361 gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg atcactttct
     6421 gcgccagcga accaccaaac tgcgcccgta gctgcgcgtc aaaatcttcg gctgacgggt
     6481 ccagtacagg taattccaga cgcgttgcca catgttcacg gcgcgctgga ctggtatccg
     6541 ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga ccgattggcc
     6601 ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc accgcatgag
     6661 cgctaatagc gaaaggttca atcaatgctg ccgcctgcgg gtcaataccg tctgcgggca
     6721 aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga tgcacgccaa
     6781 tgactgaaat tttttcacag caattggtac gcccgctttt acacgccggg cattgctgac
     6841 aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca atatttttac
     6901 ccagcccaac aatctcccca catatttcat ggcctaaaac acgtggataa ctaaaaaatg
     6961 gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac ttaattttta
     7021 ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact aattctttcg
     7081 gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt ttttgttgcc
     7141 ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt gtgaaaacac
     7201 acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa atatgagtcg
     7261 ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg cgcggggtca
     7321 tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca tcagccgcac
     7381 cacagtgcgt catattctca gccatttacg cgaatgcggc gtcttgacgc aggttggaaa
     7441 cgactatgtt attgcgcgta aacctgacca cgatgacggt ttcgcctgta ctaccgcttc
     7501 aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa accagcgcca
     7561 gttacgccca ggggaaactt tttctgaact gcaactggct cgggccgcgg gtgtcagccc
     7621 ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc aaagcgaaaa
     7681 gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc tgtttgagct
     7741 acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg atcatgatcc
     7801 ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg ctactgcgtg acaacatcgg
     7861 caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac tgctttcagc
     7921 tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct ttcattttca
     7981 ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg atgagcatat
     8041 gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg cgctccgtaa
     8101 ccatttgaat tcagctaaac aatcaatgat tcgatccatt aatgagaaca cgcgttatgc
     8161 tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt atcaaaagat
     8221 aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa atgtttttgt
     8281 gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg cgtaatgcac
     8341 aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa gaaaatatca
     8401 ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc gtgccaccag
     8461 atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc atgttgttat
     8521 tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta gcaaatttaa
     8581 cgattcgtga agaattggga ttaagtgcca ccgaaatcgg cgctttgctc tccgtgtttt
     8641 cactcgctta cgggattgcg caacttcctt gcggcccact attggatcgt aaaggcccac
     8701 gcctgatgct gggactgggg atgttcttct ggtcactgtt ccaggcaatg tctggcatgg
     8761 tgcacaactt tacgcagttc gtgttggtgc gtatcggtat ggggattggt gaagcgccga
     8821 tgaacccatg cggtgtaaaa gtcattaacg actggttcaa catcaaagag cgcggacgcc
     8881 cgatgggctt cttcaacgca gcttctacca ttggcgttgc cgtaagccca ccgattctgg
     8941 cggcgatgat gctggtgatg ggctggcgcg ggatgtttat taccattggt gtactgggga
     9001 tttttctcgc catcggctgg tatatgctct atcgcaaccg cgagcacgta gaactgactg
     9061 ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc ccgccgagat ccgctcagtt
     9121 ttgccgaatg gcgcagcctg ttccgtaacc gtacaatgtg gggaatgatg ctcggattca
     9181 gtggcatcaa ctacactgcg tggctgtatc tggcctggct tcctggttac ctgcaaacag
     9241 cctataacct ggatttaaaa agcacagggt tgatggcggc tatccctttc ctgtttgggg
     9301 ctgccgggat gctggtcaac ggttacgtta ctgactggct ggtcaaaggg ggaatggctc
     9361 cgattaaaag ccgtaagatc tgcattattg ccgggatgtt ctgttctgcc gcctttacgc
     9421 tggtcgtacc gcaagcgaca acatccatga cagcggttct gctgattggc atggcactgt
     9481 tctgtattca ctttgccgga acatcctgct ggggcttgat ccacgtcgca gttgcttctc
     9541 gcatgactgc gtcggtgggc agtatccaga actttgccag cttcatctgc gcctcttttg
     9601 cgccgatcat tactggtttt attgttgata ccacccactc attccgtctg gcactaatca
     9661 tctgcggttg cgtcaccgca gcgggggcac tggcgtacat cttcctggtt cgtcagccga
     9721 tcaacgaccc acgtaaagat taatctccct atgcccgata acattttgct tatcgggcat
     9781 ttttatggcg attaaaacgt ttcccagttc tccccgctat ctgccactgc cattttacgc
     9841 ggcgtagctg gcgtcacggt ttttaccaca gccgatgttt cacgctgctg ttgctgctgt
     9901 tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac tggcctgctc ttccagcgcg
     9961 gcggcggcgg cagattcttc caccagcgcg gcgttctgtt gagttacccg gtccatctca
    10021 gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc agaagctatt
    10081 tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa tctccgccat tgtttccccg
    10141 gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt
    10201 ttaatttcac gagccgcctg ggcgctacgc tgggccagat tacgcacttc tcccgcaacc
    10261 accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc gttcaaagcc
    10321 agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc gattttctgc
    10381 gaactggtgg agatatcgcg catagtctgc accacgttat ccaccacttt gccgccgcgt
    10441 tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt ttcggcgttc
    10501 tgcttcaccg ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc
    10561 tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct ggcaccgcta
    10621 tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata
    10681 tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc aatcggtttc
    10741 accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat tggcgctacc
    10801 agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac gatcatcacg
    10861 cccaccagaa tccacatagc ctggctgtag gaggcattgt tatcgctgac ggcgatatca
    10921 tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat
    10981 ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaatag ttggatcagc
    11041 tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg
    11101 ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc cgcctgtttc
    11161 agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc
    11221 tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg caacaacgcg
    11281 acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt
    11341 tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg taaaaggcca
    11401 aaaacagcca aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct
    11461 ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta tgaaaaggtg
    11521 taacccgcca tccatggaaa aaccaggcgc tggttatcag atactaaaaa gttattcatt
    11581 atcgcccggg atgtcttctt gtcgggaatt gcccagagca ataaattcct ctaacagatg
    11641 ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat aggcttcttc
    11701 aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg agatatagag
    11761 cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt ccatccgcgt
    11821 aaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat cgtgaaaatc
    11881 cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag tcaaattatg
    11941 ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga gcaa
//
LOCUS       DAZFAH010000143        11785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFAH010000143 DAZFAH010000000
VERSION     DAZFAH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.143"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            356..1654
                     /gene="kgtP"
                     /locus_tag="ACHJWB_003527"
     CDS             356..1654
                     /gene="kgtP"
                     /locus_tag="ACHJWB_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4315100.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1651..1974)
                     /gene="yfiM"
                     /locus_tag="ACHJWB_003528"
     CDS             complement(1651..1974)
                     /gene="yfiM"
                     /locus_tag="ACHJWB_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4315101.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2020..3375)
                     /gene="pssA"
                     /locus_tag="ACHJWB_003529"
     CDS             complement(2020..3375)
                     /gene="pssA"
                     /locus_tag="ACHJWB_003529"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4315102.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3489..6149)
                     /gene="pat"
                     /locus_tag="ACHJWB_003530"
     CDS             complement(3489..6149)
                     /gene="pat"
                     /locus_tag="ACHJWB_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4315103.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6181..6879)
                     /gene="tapT"
                     /locus_tag="ACHJWB_003531"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6181..6879)
                     /gene="tapT"
                     /locus_tag="ACHJWB_003531"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4315104.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSCFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6948..7367)
                     /gene="trxC"
                     /locus_tag="ACHJWB_003532"
     CDS             complement(6948..7367)
                     /gene="trxC"
                     /locus_tag="ACHJWB_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4315105.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7574..8611
                     /gene="yfiF"
                     /locus_tag="ACHJWB_003533"
     CDS             7574..8611
                     /gene="yfiF"
                     /locus_tag="ACHJWB_003533"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4315106.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8659..9348)
                     /gene="ung"
                     /locus_tag="ACHJWB_003534"
     CDS             complement(8659..9348)
                     /gene="ung"
                     /locus_tag="ACHJWB_003534"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4315107.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9653..10036
                     /gene="grcA"
                     /locus_tag="ACHJWB_003535"
     CDS             9653..10036
                     /gene="grcA"
                     /locus_tag="ACHJWB_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4315108.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10092..10679)
                     /gene="eamB"
                     /locus_tag="ACHJWB_003536"
     CDS             complement(10092..10679)
                     /gene="eamB"
                     /locus_tag="ACHJWB_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4315109.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10781..11662
                     /gene="yfiE"
                     /locus_tag="ACHJWB_003537"
     CDS             10781..11662
                     /gene="yfiE"
                     /locus_tag="ACHJWB_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4315110.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2934 a         2941 c         3049 g         2861 t
ORIGIN      
        1 tccccatgcg agagtaggga actgccaggc atcaaattaa gcgaaaggcc atccgtcagg
       61 atggcctttt tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
      121 cccacatatg ccagattcag caacggatgc ggtttcccca acttgcctac ttccatacgt
      181 gtcctcctta ccagaaattt atccttaagc tcctcaataa ccatttccct gctaactaaa
      241 ttcatggtta aggttgcata atgatacgca acaaatgtat aacattttct ttaccaaaag
      301 aaataaataa aagcgaccga caaaagcatc ggattacggc aggagacata atggcatggc
      361 tgaaagtact gtaacggcag acagcaaact gacaagtagt gatactcgtc gccgcatttg
      421 ggcgattgtg ggggcctctt caggtaatct ggtcgagtgg ttcgatttct atgtctactc
      481 gttctgttca ctctactttg cccacatttt cttcccttcc gggaacacga cgactcaact
      541 actacaaaca gcaggtgttt ttgctgcggg attcctgatg cgcccaatag gcggttggct
      601 atttggccgc atagccgata aacatggtcg caaaaaatcg atgctgttat cggtgtgtat
      661 gatgtgtttc ggatcgctgg ttatcgcctg cctcccaggt tatgaaacta taggtacgtg
      721 ggctccggca ttattgcttc tcgcgcgttt atttcaggga ttatctgttg gcggagaata
      781 tggcaccagc gccacctata tgagtgaagt tgccgttgaa gggcgcaaag gtttttacgc
      841 atcatttcag tatgtgacgt tgatcggcgg acaactgcta gccctactgg ttgtcgtggt
      901 tttacaacac accatggaag acgctgcact cagagagtgg ggatggcgta ttcctttcgc
      961 gttaggagct gtgttagctg ttgtggcgtt gtggttacgt cgtcagttag atgaaacttc
     1021 gcaacaagaa acgcgcgctt taaaagaagc tggatctctg aaaggattat ggcgcaatcg
     1081 ccgtgcattc atcatggttc tcggttttac cgctgcgggc tccctttgtt tctatacctt
     1141 caccacttat atgcagaagt atctggtaaa tactgcggga atgcatgcca acgtggcgag
     1201 tggcattatg actgccgcat tgtttgtatt catgcttatt caaccactca ttggcgcgct
     1261 gtcggataag attggtcgcc gtacctcaat gttatgtttc ggttcgctgg cagccatttt
     1321 taccgttcct attctctcag cattgcaaaa cgtttcctcg ccttatgccg cttttggtct
     1381 ggtgatgtgt gccctgctga tagtgagttt ttatacatca atcagtggaa tactgaaggc
     1441 tgagatgttc ccggcacagg ttcgcgcatt aggcgttggc ctgtcatatg cggtcgctaa
     1501 tgctatattt ggtggttcgg cggagtacgt agcgttgtcg ctgaaatcaa taggaatgga
     1561 aacagccttc ttctggtatg tgaccttgat ggccgtggtg gcgtttctgg tttctttgat
     1621 gctacatcgc aaagggaagg ggatgcgtct ttagtgacgg gtcagttgcc agacggtata
     1681 gccggtgctt gcaccggcga catcccaggc caaatccttc cagctccagc cgctcccttc
     1741 ggggcggcta tcccaaagct cttttgacgc ccccaaactg acagagaaca tcaatccaaa
     1801 catggcactg cgatcccggc tcatcccctg atgctgtgaa tattcatttc cggcggcgga
     1861 aagcatcgcc gaggcgataa agtgttgagc tttatcctgc ccgctccagc tatcgttagc
     1921 catatggctg catccagaaa gtaacaatag cgaacagaca aaaagaatac gcaaaacgtg
     1981 gcctccaaaa aacaaacccc gtacaggacg gggttgtgat tacaggatgc ggctaattaa
     2041 tcggtcgatg cggatacggc gcaaacggcg gatgagttta cgaaccttca ccggataatc
     2101 ggcaatactt tgcagatcgc gatagtgctt aacgatggtg gtatgctcgc ggatcagctc
     2161 cagttctttc tctcgctgcg gcgcaagctc cagttgtgga tcgtggatca aaatggcgtt
     2221 ttccagatcc agacgccagg cgcgcgggtt caggttatta ccggtgatca acatccactt
     2281 atcatcaacc cacatccctt ttaggtgata ggtgttgtcg tcatctttcc ataaccgaac
     2341 cactagctgg tcagtattga cgtaatactg caaacggctc aggaaacgac gcagattgat
     2401 ctcatagaga taaggcaatg cgccaattat cttgaaaggt tcatcttccg gaatgtagaa
     2461 gtcgttcgcg gttttatcac caacaataat ttcgaccttt ttcccttcgc gcagcaactg
     2521 gataatattg cgcacaagga ttgctggcag gttgaagtat ggcgtacaga tggttagttt
     2581 ctgctcggca caaggcataa gatggaaaat ggtcttgttc aacagactcg atttccccag
     2641 ccccactagc ggcgttacgg aaagctgatc gttgtcggca tcgccctgga aatgataagc
     2701 ggcatcacgc agctcctggc ggaacagacg aatatcgttc ttgatttccg ggctttttgg
     2761 ccgattaaca tcatccagac gattaacgcc gcggccattc ataatattct gtgtaaccca
     2821 ttcaaacata atgtctgaca tcttacggtt acggatcaga tgataacggt cgtagcgata
     2881 tttatcgtgc tgatgcaggt aaacatcgtt caggctggca ccgctataaa gtacgctatc
     2941 gtcgatgata aagcctttaa agtgcagaac accaagggct tcacgggtat tgattggaac
     3001 gccataaacc ggaacatcta cgcccggatt ttcctgcgcc atgcggcagt accagtcagc
     3061 gttagtgtta gatgccgcag cgccaatgcg tccacgttgt gcacgatgcc agtcgaccag
     3121 cacccgcaca tccagttccg gacgctgcct tttagcttca tacaacgcgt tcagaatgcc
     3181 tttgccaccg tcatcctgtt cgagatacag ggcgacaatg caaatgcgct gcttcgcgct
     3241 ggctattttt tccagcagcg tctcccggaa gtcggcggga gcgtaaaaga aatcgacatc
     3301 atcaactgat tgagaaatct tgggtagttg ggcaaggtgt tgttgatgtt tattacgctt
     3361 aaattttgac aacatcacag tgcatttctt ctctgttcat tgaagggtcc tctgtgcaat
     3421 gcagacgaca taagcgggca ataataacac cagtcccgat taagtggtca acatttccag
     3481 taccttactc atgattcctc gcgttgggca agatttagcg taagcccaac gatcccctct
     3541 tcgagctgga tatcaacgtt aaacccgagc ttgcgggcta gcgccaccat gccacgattg
     3601 tttggcatcg taataccatt cagacgttgt agtccgtgat ctcgcgtata ggtaatcaac
     3661 ttttccatta agcgtcgacc taagcctaac cctttgagat ccgagcgaac cagcacagca
     3721 aattcggcat cgatgttatc aggatcggaa atcgcacgcg tgacgccgag gatctcttcc
     3781 gtttgatcaa tacgtcgtac cgctacaaac gccatttccc gatcgtagtc gatctgtgtc
     3841 atgttggcta aatcttcatg ggtaaattcg ttgatctcgc taaagtagcg gtaataaaga
     3901 tcttctttgg tgactcgcga aatgaattgc tgaagttgtg gctcatcttc tggcaaaatc
     3961 gggcggaaca agcagcgttc accgtttttc aattctaccc attcttccag ctgatgcgga
     4021 taagggcgca ctgccagccg actctcgtta tcgccttcaa acggcgagat atccagcgtg
     4081 acatccagcg cggtaaattc actgccagaa gccagcaaag gatgaatatc cagacgctga
     4141 atttccgggc aatcgacaat caagttggaa acctgcacca gaagctggct caagcctgca
     4201 acatccaatg ggcgtagcgc actgcgcgca cgaatctttt tacttttgat cccctgaata
     4261 accagatagc gggccaggtt catgttcagc ggcggcagtg cgacgacggc ttgatcttca
     4321 ggacgccact ccacaccgcc ttcacccagc atgatcaacg gcccgaaaac cggatcgtgc
     4381 tcaaccacaa cccgcaactc ctgagcgcca gcacggttag ccatactttg caccaacagg
     4441 ccgtggaccc gcgcctgtgg ccaggccatt tttacgcgat cgaaaatagc gttcgccgct
     4501 tgctggactt cattggctgt acgcaggtaa agcatgacgc cctgaacttc cgatttatgt
     4561 ggaatatccg gcgaacgcaa tttcagcgcc accggataac caatctgttc ggcaatatgc
     4621 accgcttcgg tgctatcgct ggcaatccag gtagggagcg tgttcatgcc atacgcttgc
     4681 aggatgggct gaacttcatg ggtatcgagc gacgtagccc cttcggcaat cgcctgttgc
     4741 aacagaagat gcgcttctgc ggtattggaa gtcagattgc tgggcaacgc cggcgtttcg
     4801 cgtagttgct tctgattacg ccggtactcc accatatgca taaaagcagt gatggttcct
     4861 tccggggtac ggtaggtcgg cagcccggct tcgctgaata aacgtcgtgc ctcttgcgag
     4921 gagtgctcgc cgcaccagtt cgtcagcaaa gagacatatt tgctgcgggg atgatgcttt
     4981 accgcttcaa ttaatacttg cgcgctttct gttgcgggag cagcggcgct gggcgaatga
     5041 ataaccatca gcgcatcaaa atcctggctg tggagcagaa tgtccagcgt tttgacataa
     5101 tgctcgctgc tggcgtcatc gcgcagatcg agagggttag agactgccac atgttctggc
     5161 aatgcatcgc gcaatttctg gcaggtttct tcgcttagcg ttgccagctt gccattgcgt
     5221 gaccataagg catccagcgc cagcgcggca ggcgcagcac cgttgctgat aatcatcaac
     5281 cggtcgccac gcagcgggcg catatggcta agtgtttcca ccgccgaaaa cagttcgtgg
     5341 gtatcctgta cccgcaacaa accggcacgc tgaatagccg catcccatgc cgggtccatt
     5401 cctgccgtcg tgttgagcag tcgctgtgcc gccgggctac gtccgctttt aatcaccaga
     5461 atcggtttat tacgcgaggc actacgggct gccgaaacaa agcgtcgcgc gtcgcttaac
     5521 tgttcgagat agagcaggat ggcgctggtt ttactgtcgc gcgccagata gtcgagcaat
     5581 tcatcaacgt cgatatccag gctgtcgccg agcgcaataa agtaggaaaa gcccatctca
     5641 cgctgttgcg cccagtcgag gatggtgttg gagacggctg ccgattgtga aataaacgcc
     5701 agcttgccgc gtttaatcgg cacaggcgaa aagctggcat tcagaccttg ccagggagcc
     5761 agtaaaccca gactgtttgg tccaagcagg cgcatgttat ggcgcagggc gcaggcgcgg
     5821 agatcttcgt gttgcgatgc cggggcggaa agaataatgc aggttttaca gcctttctcg
     5881 ccgagctctt ccagaagagc aagattacgg ctggcattgg tacataaaac cgcaaggtcg
     5941 ggtgtaaagg gcaagctggc aatatccggc caggccaaca cacccaacac cgctttccag
     6001 gctggcgtca ccgggagtac cggtccgtta aagcctcccg ccagcaggtt acgcatcatc
     6061 aggtaacctg cgcgattggg tttcatcgac gcgccaatta ccgctatcga ttttggtcgc
     6121 agtagtgctt ccagtcctcg ctgactcata ccggtctccc gcagaagtga ccgaatgatt
     6181 ttaaacgctt tctaactgtt ctgctgtgat gctacccaga tgttgcgttt ttcctgccag
     6241 atagcgtgtt ttaaagcggg taaaatgctc gcctaaccct gctgccgccc cggtatcgcc
     6301 ggccatatct aacagtgcga tggctacctc ggcagtacaa tattggcctg cagcctgggc
     6361 ttcacgcagg cgataggcag aaagccggga aagatcgacg gaaatgacgg gaagattatc
     6421 cagatacgga cttttacgaa acatcttgcg agcttccggc caggtaccat cgagcatgat
     6481 aaacagcggt ggcttaccgg caggtggtgt gaagatcact tcccgttgct catcagcata
     6541 cgaggcggga aagaccacca ttggctgata atccgggttt tgtaccagat ccagcaaatc
     6601 ctgcgagggt tcggtacgcg accattgaaa cgcaacggta tcaggcaaaa tatcagcaat
     6661 cagacgcccg gtattactgg gcttcattgg ctcggtgtcg aacatcagca aacagaagca
     6721 actttttgct tgtgctgggg taattgtcga acagagacat aatttctctg gcaaaagaca
     6781 gcgttggcag cgacgaacgc gattaccgcg ggcaagaaaa ggacgtgttg cgcgcgcaat
     6841 acgctcggcg cgtaactgga gaacagcgtt ttcggtcata agagagcgtc gaaaaaacgc
     6901 cattgtcgca gaggagaaaa cggggcacaa gatgcgcccc ggtaagatta aagagattcg
     6961 ttcagccagc tatcgaacgg cgctttcggt actgcgccat taagcatgtc gacaacctga
     7021 ccgtttttga aaatcatgat cgtcggtata ctacgaattc caaagcgact gctcaattca
     7081 cgttcagctt cggtattcac tttcacaaag cgcactttac cgctacgctc ttgcgcgaca
     7141 tcttcaaaaa ttggtgcgaa attacggcag gggccgcacc acggtgccca gaagtcgatc
     7201 accacaggta gatcatcctt cagcaatttg tcgagtgttt caccggtcgc attaatcacc
     7261 tctccgtcaa acaagtcgtg accgcagcgt ccgcattttg ccgcatcttc gatccgatcg
     7321 tcgggaatgc gattgatggc ctgacaatgg gtacaaacgg tattcataac taacctcggg
     7381 atgagtaagg agacttatcg gcaacgacgc cggtatgttt ctaatatgtt acatattatc
     7441 ggggacattg ttccagacaa caatctgttt tattcaatag gtatgatagt cgcaggcttt
     7501 ggtacgaaat catggcgaac cgcgcggact tcgggtaatc tgcgcgcttc gcgcagcgct
     7561 ggtggagaaa agcatgaacg atgaaatgaa aggtaaaagc ggcaaggtca aagtgatgta
     7621 tgtccgcagt gatgatgatt ctgataaacg tacccacaac ccgcgtaccg ggaaaggggg
     7681 cgggcgtcca ggaaaatctc gtgctgacgg tggccgtcgc cccgcccgcg atgacaaaca
     7741 gagtcagccc cgtgaccgca aatgggaaga ttcgccgtgg cgcacggttt cccgcgcgcc
     7801 gggtgatgag acgccggaaa aggccgatca cggtggcatc agtggtaaaa gttttattga
     7861 tccggaagtg ttgcgtcgtc agcgtgcgga agaaacccgc gtctacggcg aaaatgcatg
     7921 tcaggcactt ttccagagcc gtccggaagc gattgttcgc gcctggttta tccagagtgt
     7981 aacgccgcgt tttaaagaag ccttgcgctg gatggcagca aaccgcaaag cgtaccatgt
     8041 ggtggatgaa gcggaattga caaaagcgtc aggcacggaa catcacggcg gcgtttgctt
     8101 cctgatcaaa aagcgtaatg gcacaaccgt gcagcagtgg gtaagccagg caggcgcgca
     8161 ggattgtgta ctggcgctgg aaaacgaatc taacccgcat aacctgggtg gcatgatgcg
     8221 cagctgcgcg cacttcggcg tgaaaggcgt tgtggtgcag gatgcggcac tgctggagtc
     8281 gggggcggct atccgtaccg cagaaggcgg cgcagagcac gttcagccga ttactggcga
     8341 caacattgtt aacgtgttgg atgatttccg tcaggcaggt tacaccgtag tgaccacttc
     8401 cagcgagcag gggaaaccgc tgttcaaaac cagtctgcca gcgaaaatgg tactggtgct
     8461 gggtcaggaa tatgaagggt taccggatgc cgcacgcgat ccgaacgatc tgcgcgtgaa
     8521 gattgacggt acgggcaacg ttgccgggct gaatatctct gttgcaaccg gcgttttgct
     8581 tggtgaatgg tggcgtcaga ataaagcctg ataaatcagc cgggtggcaa ctctgccatc
     8641 cggcatttta ccgcaaattt actcactctc tgccggtaat actggcatcc agtcaatcgg
     8701 cgtctcgcca cgttgttcca gccactgatt tgccagcaca aaatggttgc agccaaagaa
     8761 tccacgatgc gccgaaagcg gcgacggatg cggtgctttc agtacatgat ggcgttgctt
     8821 atctataatc gcccctttct tttgcgcatg tgatccccac aacaaaaaca ccacgccttc
     8881 gcggtgctgg ttaatcaggc tgatgacttt atcagtaaac gtctcccaac cgaggctggc
     8941 gtgggaatgc gcctgacctg cgcgaaccgt caacacagta ttgagcagca gaacgccctg
     9001 acgcgcccag ctttcgagat agccatgatt agggcgggtg aagcccggaa tagtattttc
     9061 cagctcttta tacatattca ataatgacgg aggaatggca atgccgggac gaacggaaaa
     9121 tgccagacca tgcgcctgtc ccggtccgtg ataaggatcc tggccgagaa tcaccacttt
     9181 aacgtcaccc aactcggtaa agcggaacgc gttaaagaca tctttttgtg gtggatagat
     9241 agtgacgccg gactgccgct cgctggcgac ggtctgaagg gtattaagaa aataaggttg
     9301 ttgcttctct tcagccagca cgtcatgcca ggttaattcg ttagccatct cgctctcctg
     9361 cgaatcttca atccgcctag cttaactgct tctttctaca gaacaaaatc atgctcattt
     9421 tttagactta aacgagtgca aaaaaatgta aattggtaaa atttcacacc cgcataagtt
     9481 gatgtaaaac aataaaatcc atttacgacc acaatcgatg tgtggttttt attgatttaa
     9541 atcaaagaat caagggtggt tgaggagtat atatacactc aagcaacaat ggttttacca
     9601 attggccgcg acaggctgaa caaatcaaat aattttgccg gggaggcatc acatgattac
     9661 aggtatccag attactaaag ccgctaacga cgatctgctg aactctttct ggctgctgga
     9721 cagcgaaaaa ggcgaagcac gttgcatcgt tgcaaaagca ggttatgcag aagatgaagt
     9781 ggttgcagta agcaaactgg gtgacattga ataccgtgaa gttccagtag aagtgaaacc
     9841 agaagttcgc gttgaaggtg gtcaacacct gaacgttaac gttctgcgtc gtgaaactct
     9901 ggaagatgca gttaagcatc cggaaaaata tccgcagctg accatccgtg tatccggtta
     9961 tgcagttcgc tttaactctc tgactccgga acagcagcgc gacgttatcg ctcgtacctt
    10021 tactgaaagc ctgtaaggct taagtagcaa gttactaagc ggaagagggc gacctcttcc
    10081 gctttttttc gttaatagaa aatgcgtacc gcgcaataga ccagcaacag ggcaagcacg
    10141 atatttaact ggcgaccata ctggcgaaac aatcgctgaa acagatgccc cgccagcgcc
    10201 cagcatacat tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc aactacccag
    10261 cttagcgcct gtgtttgcgg cagaacaaac gtcgacagtg ccgtaacacc gtacaaaatg
    10321 attttgacgt tcacaaactg caaagcaaag ctggcccaaa agctgattgg ttttgcctga
    10381 agtccgtctt cctttgttgg gctggtggcg attttccacg ccagccagac aatatatgcc
    10441 gcccccgccc aactcaaaag gtgtaccgct gccgggtcaa tcactgccag tgaaaatgaa
    10501 atgcccgcac acagtaacat cacaatcaaa aatcccagac tcatccctgc cagcacgcgg
    10561 gtactttgac gaaatccatg cgacgtagca gagctaaggg cgagaatatt gttcggtcct
    10621 ggcgtcatag cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt cggtgtcact
    10681 tgttatctcc tctgttttgg tagacagtac ccgacacgga acatttaaaa aatgaatgtt
    10741 tttatattct agattcgata ttttcgatag gtttggggtt atggatctgc gccgttttat
    10801 tacgcttaaa accgtggtgg aagagggttc ctttttgcga gcttcgcaaa aattgtgctg
    10861 tacacaatcg acggtgactt ttcatattca gcagcttgag caggagtttt cagtccagtt
    10921 atttgagaaa attggccgac ggatgtgcct tacccgcgaa ggaaaaaagt tgctgccgca
    10981 catttatgaa cttacccgag tgatggatac gcttcgtgag gctgccaaaa aggagtcaga
    11041 tccggacggg gaactacgcg ttgtttcggg cgaaacgttg ctctcttacc gaatgccgca
    11101 ggtgttgcag cgttttcggc aacgcgcacc aaaagtgcgt ttatcgttgc agtcgctgaa
    11161 ctgttacgtg atccgtgatg cgctactgaa tgatgaggcc gatgtcggcg tcttttatcg
    11221 cgtagggaat gatgatgccc tgaaccgacg agagttgggt gaacaatcgc tggtgctggt
    11281 ggcttcacca caaattgtag atgtcgattt tactgagccg ggaagacata acgcctgtag
    11341 ctttattatc aatgaaccac aatgtgtctt ccggcagata tttgagagca cgctgcgtca
    11401 gcggcggatc acggtggaaa acaccattga gctgttaagc attgaaagta tcaaacgttg
    11461 tgtcgcggcg aatatcggtg tcagttactt gccccgcttt gcggtggaaa aagagcttga
    11521 gagcggagaa ttaattgaat tgccttttgg cgaacagtcg cagaccatta cggccatgtg
    11581 cgcacaccat gctggaaaag cggttagccc ggcgatgcac acttttattc agtgtgttga
    11641 agagagtttt gtggctggat aaaaataatg tcgggattaa acccggcatt taacttgtcg
    11701 acaaagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatccggc ctacaagatc
    11761 gtgcaaattc aatatattgc atgag
//
LOCUS       DAZFAH010000144        11470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFAH010000144 DAZFAH010000000
VERSION     DAZFAH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.145"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..1704
                     /gene="mppA"
                     /locus_tag="ACHJWB_003538"
     CDS             91..1704
                     /gene="mppA"
                     /locus_tag="ACHJWB_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4315111.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1755..2786)
                     /gene="ynaI"
                     /locus_tag="ACHJWB_003539"
     CDS             complement(1755..2786)
                     /gene="ynaI"
                     /locus_tag="ACHJWB_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4315112.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3030..3287
                     /locus_tag="ACHJWB_003540"
     CDS             3030..3287
                     /locus_tag="ACHJWB_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4315113.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3337..4287)
                     /gene="uspE"
                     /locus_tag="ACHJWB_003541"
     CDS             complement(3337..4287)
                     /gene="uspE"
                     /locus_tag="ACHJWB_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4315114.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4439..5191)
                     /gene="fnr"
                     /locus_tag="ACHJWB_003542"
     CDS             complement(4439..5191)
                     /gene="fnr"
                     /locus_tag="ACHJWB_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4315115.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(5386..5901)
                     /gene="ogt"
                     /locus_tag="ACHJWB_003543"
     CDS             complement(5386..5901)
                     /gene="ogt"
                     /locus_tag="ACHJWB_003543"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4315116.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRNYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISVVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5912..7438)
                     /gene="abgT"
                     /locus_tag="ACHJWB_003544"
     CDS             complement(5912..7438)
                     /gene="abgT"
                     /locus_tag="ACHJWB_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4315117.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNNDEKLQTLTESQRFGLRIAGVLSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFDPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(7475..8920)
                     /gene="abgB"
                     /locus_tag="ACHJWB_003545"
     CDS             complement(7475..8920)
                     /gene="abgB"
                     /locus_tag="ACHJWB_003545"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4315118.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNHTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(8920..10230)
                     /gene="abgA"
                     /locus_tag="ACHJWB_003546"
     CDS             complement(8920..10230)
                     /gene="abgA"
                     /locus_tag="ACHJWB_003546"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4315119.1"
                     /translation="MESLNQFVNSLAPKLSYWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10406..11314
                     /locus_tag="ACHJWB_003547"
     CDS             10406..11314
                     /locus_tag="ACHJWB_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4315120.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         3013 a         2805 c         2824 g         2828 t
ORIGIN      
        1 gtgtcacttt tttgatggaa gttgtttgca tttctttaag gcgaaacaaa taattacgca
       61 tcaattttaa tgtcggttag agggaaactt atgaagcact ctgtttcagt cacgtgttgt
      121 gcgctgttgg tcagcagcat ttctctttcg tatgctgcag aagttccgag cggcacagta
      181 ctggcagaga agcaggagct ggtgcgccac attaaagatg agcctgcgtc gctggatccc
      241 gctaaagccg tgggcctgcc agagattcag gtcattcgcg atctgtttga aggactggtg
      301 aatcagaacg agaaagggga gattgtcccc ggcgttgcga ctcagtggaa aagtaatgac
      361 aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg cacaccggta
      421 acggcgcaag attttgtcta cagctggcaa cgtctggtgg acccgaaaac gctatcgcca
      481 tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat tgatggtaaa
      541 gctacgccag accagcttgg cgtcaccgca gttgatgccc atactttgaa aattcagctt
      601 gataaaccgt tgccgtggtt tgtgaattta accgctaact ttgccttctt cccggtgcaa
      661 aaagccaacg tagaaagcgg taaagagtgg acgaaatccg gaaatctgat cggcaatggc
      721 gcttatgttc ttaaagatcg cgtagtcaat gaaaaactgg tcgtggtacc gaatacccat
      781 tattgggata atgccaaaac ggtgctgcaa aaagtgactt tcctgccaat taatcaggaa
      841 tccgcagcca ctaagcgtta cctcgcgggg gatattgata tcaccgaatc cttcccgaaa
      901 aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc gccgcagctc
      961 gggacctatt attatgcgtt taacacgcaa aaagggccga cggcagatca gcgcgttcgc
     1021 ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtgtt gggaacgggc
     1081 gaaaagccag cgtggcattt tacaccagat gttaccgccg gatttacgcc ggaaccttcg
     1141 ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt gttgagcgca
     1201 gctggttatg gtccgcaaaa accgctgaag ctgacgcttt tgtataacac ttcagaaaac
     1261 catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaaccttgg cgtagatgtt
     1321 aaattgcaaa atcaggaatg gaaaacctat atcgatagcc gtaacaccgg caattttgat
     1381 gttatccgcg cctcatgggt gggggattat aatgaaccct ccactttcct gacattatta
     1441 acgtcaacgc attcaggaaa tatttcacgc tttaacaatc cggcatacga caaagttctg
     1501 gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa cgcggcagaa
     1561 aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa tggacgatta
     1621 atcaagccgt ggctgaaagg ttatcccatt aataacccgg aagatgttgc gtacagccgg
     1681 actatgtata ttgtgaagca ttgatgtgat gggaactggc gttacccttg tggataacgc
     1741 cagtgatgtc tgatttagcg cccctgttcc ggcggtgtaa tgttatccat atacagcgtc
     1801 tggctgggga aggcaaagtc cgcgccgtgt gactgcacaa tatcgataat cttcaaataa
     1861 acgtcttgct gtgcagcaag ccattcagcc cataccgtgg ttttggtaaa gcaataaacc
     1921 ataatattca atgacgagtc ggcaaactgg ttgaaataaa ccagtaaggt ttgtcgctgg
     1981 tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac gcccactttt
     2041 gccgcatcct cataacgtaa accaatggtc gtggtaatgc ggcggttggt cattcgtcct
     2101 gggttttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg acgattatca
     2161 aaggtcgtaa ttttggtaat tcgccagcca atttccgcta ctgtaccttc gatatttctg
     2221 tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag catgatcccg
     2281 gaaaagaaat tactcagaat atctttaccg gccataccga cagccagacc accaatacca
     2341 ccaaaggtca gcaagccaga aaggctcatg ccgaaatgtt cgccataaag cagaacaagc
     2401 accacaataa tggtgatttt gataatgcgc gacataatcc gcgcactggt gatatcgcga
     2461 ccttttttaa tctgctgttt ttcaaactga ttaatcagca aaaatagctt aatcgtcaga
     2521 ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggggtgat aaatttgagt
     2581 ttatagtttt ctataacata attaataatg ctaccaacag cactgataat tatggtgtag
     2641 attaaaaatt gcaccgcatg gaataaaaaa ccttttcttt tacgatttcc gcggcgaaac
     2701 caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag attaagcgca
     2761 ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata attgtcgtca
     2821 tgccggaaac ccctggcggg gctattttac cgcgacaatt cattcagatc atcaatagtc
     2881 agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa tccagctaag
     2941 aggtgaggtt ttcagagcag acaacggtga aatgtcatgg tattgttacg tttaggtaac
     3001 aagaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag tcagcgaaag
     3061 ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc
     3121 gcgcgactat cggcggcgtt atagaacagt acaatattcc gctgtctgag tggacgacat
     3181 caatgtatgt gattcagtca tcgatgattt ttgtttatag cctggtcttt actgtgttgc
     3241 tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta aaaaataggc
     3301 ccgataactc gggccttgtc agttattgaa gagtcgttaa tcgtcttctt cgtcatccag
     3361 ttcaacgggt gtctgatact ggtcaggttt aatgaccaac agatcgcagc gaagatgatc
     3421 aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc gtcctaccgt
     3481 gcccagaacc acaatccccg cctgtaagtg ctccgccaaa tcaggaatca cctcttctgg
     3541 cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc gcagagcttt
     3601 cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcggat caaattccgg
     3661 cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat gaacttcggt
     3721 atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat tgagcgcatt
     3781 atggtacggc tcttcactgg cgagattcac cgccaccagc gccttacctc cttccggcca
     3841 cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacagat gccagtccgt
     3901 tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta gcaccaaatc
     3961 gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat gccagaccac
     4021 tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt ggatccaggc
     4081 tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg agagcagggt
     4141 ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc caccaatccg
     4201 ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag gatcgataac
     4261 aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg tcaacgcagt
     4321 ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc ttctgccaga
     4381 tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg cggaaaaatc
     4441 aggcaacgtt acgcgtatga ccagcaagct gggccagcgc atcgttattt tcgatggtga
     4501 tgtatttacc tttgactgcc agcatgccgc ttttctggaa gcgacccagc agacggctga
     4561 tggtttctac cgtcaggccc agatagttac cgatatcgcc acgagtcatc gtcaggcgga
     4621 attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag atgaatgcag
     4681 ccagacgttc ctcggcattt ttcttcgaca acagcaggat catgtcctga tcgcctttga
     4741 tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta ccggacaaat
     4801 cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc tgcgcgaagc
     4861 tcgggtgatg gccgctgccg atggcgtcaa atcccaccag gtcgcctgct aaatggaaac
     4921 cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta ccggagcgga
     4981 tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct ttctgaatag
     5041 gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg agtgtgaacg
     5101 ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg ccagactgaa
     5161 tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa ttgatatttg
     5221 tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac agcagagaat
     5281 tccgatatta gatgtaaata tatgtctatc tatttgaaaa accttaagtt gttaagggta
     5341 actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca gcaaaagata
     5401 accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc cggtcatggt
     5461 gccgtttcgg ccaataaccc gatggcaagg tacgacgacg ctgatgggat tcgatccgtt
     5521 tgccgcacca acggcacgcg ccgcgccagg gcggcccaat tgttcagcca gttggccgta
     5581 atgcattacc tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg
     5641 cgtcccccct gtggcagtgg gaagcgtatc aataatgcta agattaccgg caaaataatt
     5701 acgaagcttg tcgcttaaac cgcctgggtt ggtggcagaa atgcgctcat agccttcttt
     5761 gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc attcaaccgc
     5821 ccgcagacga aattgctcat cgcaaatcac ccacagtggc cccagtggcg tggcaatttt
     5881 ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa atccccggac
     5941 ctatcggcag accaacaaga taccacgcca gcaacatcag cagccatacc accaaaaaga
     6001 taagcggata gggcaagact aacgaatagt aagtacccag tttcgcgtct ggtttgtagc
     6061 gttgcaggaa tccaagaaac agtggaacaa aaggagatac cggcgctaaa ggcaatacgg
     6121 atgagtcggc aatacgaaag aggatttgcg caaatgccgg gtcaaagcca agtagcataa
     6181 acattggtac gaaaatgggg gcaagaatcg accagattgc ggaaccgctg gcaataaaca
     6241 tgcataagaa agaggaaagc aacgccagac cgacaaacgc cgggatgccg ctaagccctg
     6301 aactttccag tatatcggtc agccccacgg cgatgaattt ccccatgttg ctccagttaa
     6361 acatggcgac aaattgggcg aggggaaaaa ccatcacgat aaatcccgcc atctctttca
     6421 tcggttcaat cattaaatgc ggtaaatccg cctgacgtcg aattgtgcgg gtagcgatgc
     6481 cataagccag cgagacaaca aagaaaaaaa gaatgatcag tggcacgata cctttaataa
     6541 agggtgatgg catcacggtg tgattaatcg gatcgcgcaa tatcccgttt tccgggatca
     6601 ccatcagtgc aatcgcagca ataaaaagta gcgataagac acctgctatg cgtaaaccaa
     6661 aacgctgact ttcggtcaat gtctgcagtt tctcatcgtt gtttccctgc cattgaccta
     6721 accgtggctc gatgattttg tcggttatca ggccgccaac aatcgtcagt acgactacgg
     6781 agctggccat aaaataccag ttatcaatta cactgacgtg catttgcgga ttgaacgcag
     6841 ccgccgcttc cgtgctgatc cccgacagca acacgtcggt tgtgacaatc agtaaattag
     6901 ccgtaaagcc gcaacctacg cctgcaatgg cagccagtaa acctgcaact ggatgcctgc
     6961 ccaccgccag aaaaatcagc gcacccatcg gtggcatgat cactaacgcc gcatcggaag
     7021 aaatgtggct gaaaaaagca ataaacagca ccatataact ggcgtagcgg gcattaacat
     7081 gcgatgccat tttaaccatt agcgctggca gtaagccgac gcgctccgct aggccggcac
     7141 ctaaaaccag cgccaggatc gctccaagtg gagcaaaacc gctaaagttt ttaatcacat
     7201 tgggtaaaaa ccagtgtaat ccttctacac tgagcaggtt tttcaccacc accggcgtac
     7261 catcggtcgg gtttttcgca ctgacgccaa aggctgacaa aattgccgtc gtcaccatga
     7321 gtacgataat caaatagata aagagcaaaa aaggatgggg aaccttgtta ccaattcttt
     7381 cgacccagcc atagagcttc ccggattggg aggacgacgg tatggatgac atactcatgg
     7441 gcattcctcg gttgttgtgt ttgcgttgtt gttgttattt taaaggtgac ggtgtcacgt
     7501 ttttcgggat agggcagtga tacggttgcg tgtccgtaac ttgctgatgc tcttgttggc
     7561 attcttgcaa tagccctgaa tcaatgaaga gattgactgt ggttgctgcc atagttttcg
     7621 ccgccagcag cattccttta tgagcaatag atgttcgccc ctggctaacc agttgccacg
     7681 tatgtagcgg tgtaccgacg gcaaaacagg ggctgaaaca ctgggcaaca ggcagtttcc
     7741 aactgacgtc gccgacatca gtcgatgccg caagcacgtt atcggtggcg gcatatggag
     7801 cgacctcatt cgccagtacc gtttcacgat gacgtagtgc aaaagccttg ccgttttcgc
     7861 caccggttgc ggcgatatta ttcagactgt tttgccgatc gttgggggtg agcgtagcct
     7921 gaatttgttt cgcaaaagcc agttcttcgg agttccattc cggggtacca aaatgggata
     7981 gggcctggta catggcattt tctaaggtgt gattcgggag ataactggaa caggctttgt
     8041 cgaagcggca ttcaaccgtg gtttcggtca tcaatgccgc accttcggcg attttggcga
     8101 cccgatcata aatatgctgc acgtcggtca tttcgggggc gcggataaga taaagcactt
     8161 ctgcctgcgc ctggaccacg ttgggcgaga tcccgccgct atttgtgatg gcatagtgta
     8221 cgcgcgcttt ttcaataata tgttcgttga ggaagttggt gccagtggtc atcaacgtta
     8281 cggcatcaag ggcgctgcgt cccaaatgag gggaattcgc ggcatgtgct gcgatccctt
     8341 taaagcgcca tgatgcctga atgtttgcca gcgtgcgggt attgaacata ccggcaaagg
     8401 cttccgggtg ccaggtgagt gccgcatcca catcatcaaa taccccctcg cgaaccatga
     8461 acgttttacc cgagccgcct tcttcgccag gacaaccata aaagcgcacc gtgccgcctt
     8521 gcccatattg ttccagccat ttcttgacgg ctattgcagc ggcaaaggcg gcggttccca
     8581 gcaaattgtg tccgcaaccg tgaccatttt caccgggcgt cacggatgta ggttgcgcgc
     8641 aacctgcttg ctgacttaaa cctgccaggg cgtcatattc tcccagcagg gcgataaccg
     8701 gtttgccttg accaaacgaa gcaataaagg catttgggat attgcctacg ttgcgggtaa
     8761 cggtgaagcc tgcagattcc agcgccgaag ccagatgctc cgctgaccag aactcttcaa
     8821 aacgtgtttc tggatgatcc cagatttgat cggcaatatc ggtataacgt tggcgatcgg
     8881 cttcaatcgc atcgtcgata aaacgataga tttcctgcat cagatacctc gcgtccaggg
     8941 aaaattgagc gcggtgcgcg ccagcgtttc gacggcaata gcgagaacct gctcgtcaaa
     9001 atcgaatttt tcgttgtgat gacctgccgc cagctgtgtg ccaaacacca cgtaggaggc
     9061 ttgcccttga tgttgctgca cgcgggccat cattaatgtg gcatcttcgg aacccgcagg
     9121 cgcttcaaca cgttcaatgg cctgattgac ccccgcgacc tgagccgcct gactttgcaa
     9181 ccatgcgacc cattgcggcg aaggagaact ggcggtagct gcacccatca gacgagtttc
     9241 aacgccgaca ccatacatgg ttgctgcgcc ctgaatcgct tgttgtgcac ggtcaaaaac
     9301 atattgatta atgacgtcgc tggccccgcg tgtttccact ttcagcaacg ccgaggcagg
     9361 aacaacgtta cgaccgcttc ctgcctgcat aacgcccacg tttactctgg aagctccttc
     9421 gctgtgcggg gcgattgcat gcagtgcaag agtggcttgt gctgccgcca acaaggcatt
     9481 gtgaccgtct tctggttttg cgcctgcgtg agcggcggta ccggtgaagt gcgcgtcaaa
     9541 tttggtggtt gccataaaat tatcactgcc gcacaccacg gtgcccgcag gtacgccagt
     9601 gccaatgtgc acggcagtaa aataatcaac atcatctacg acacctgcat cgaccatcgc
     9661 ccgcgcgcca cgcgtacctt cctctgcagg ctgaaaaatc agtttgatga cgccatgtag
     9721 tccggactcg aattgtttaa gggtatgcgc cagcccaagc ccaatggcgg tatgtccatc
     9781 atgaccacag gcatgcatca ttccggcgtt acatgacgca aaaccgtcgc ggtaggggcg
     9841 atggctgaca tcctgctctt cactgagatc cagcgcgtcc atatcgacac ggaaagccat
     9901 caccggaccg gggcgaccgg tatccagggt ggcgacgata ccagtgaaac caccttcaaa
     9961 aaccgcaatc cattgtgcta gcgcaccctg ttgacgagcg cgctcgaatt cgcgttgtag
    10021 agtgagttca tcaggtagtc ccatccggct actttcatta actacttcgc ggcccagcgc
    10081 cagtgaatag ccgagctggt gcaattcttc cgcaacaagg gtggcagtgc ggaattccac
    10141 ccagccagac tctgcatagt gatgaaaatc acgtcgccag tacgataatt ttggggcaag
    10201 cgaattaaca aattgattca aagactccat aacctttccc gtcatcagta aaaagtgtga
    10261 cccggttcac gtagcgatag tttttactta tcactaactg atttttcaca gttttaaccg
    10321 ttcataaatt accctgacac aatcatctgc attaaagtag atgccagttt ctttggtctg
    10381 ataaataacg gttatcggtg gcgtcatggc ttttcaggta aaaattcatc aaattcgggc
    10441 ttttgttgaa gtggctcgtc agggcagcat tcgcggagcg agccgaatgt tgaatatgtc
    10501 gcaaccggca ctgagtaaat ctattcagga gctggaagaa gggttagcgg cgcaactctt
    10561 ttttcgccgt agtaaaggcg tgacgttaac tgatgccggt gaaagttttt atcagcacgc
    10621 cagtcttatt cttgaagaac tgcgcgcagc ccaagaggat attcgccaac gacaagggca
    10681 actggcaggg cagattaata tcggcatggg ggccagtatt tcccgcagtc tgatgccagc
    10741 tgtcatatct cgttttcatc agcagcatcc gcaggtaaaa gtacgcatta tggaagggca
    10801 actggtgtcg atgattaatg aattgcgtca gggagaattg gatttcacca tcaataccta
    10861 ttatcaggga ccgtacgacc acgaatttac ttttgagaaa ttactggaaa agcaattcgc
    10921 gatcttttgc cgcccgggac accccgccat tggtgcccgt tcgatcaaac agttactgga
    10981 ttacagctgg acaatgccga cgccacacgg cagctactac aaacagttga gtgaattgct
    11041 tgacgatcag gcgcaaacgc cacaggtcgg tgtagtctgc gagacgttct cagcctgtat
    11101 cagtctggtg gcaaaaagcg attttctcag catactgcct gaagaaatgg gctgcgatcc
    11161 cttgcacgga caggggctgg tgatgttgcc ggttagcgaa attttaccga aagcggccta
    11221 ttatttgatt cagcggcgtg atagtcgcca gacaccactg accgcgtcat taatcacgca
    11281 attccggcga gaatgcggct atctgcaaag ttaaaactgc ataaaaaaat agagtctgtc
    11341 gacatccgcc agactctaca gtacacacag cagtgcatcc gcgtcttaaa tccggcattg
    11401 tctcctctgc gccggtgact gtgttataac ggttttaacc tttagttgcc aattttcttc
    11461 agtgacagat
//
LOCUS       DAZFAH010000145        11415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFAH010000145 DAZFAH010000000
VERSION     DAZFAH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.146"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            294..1160
                     /gene="folD"
                     /locus_tag="ACHJWB_003548"
     CDS             294..1160
                     /gene="folD"
                     /locus_tag="ACHJWB_003548"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4315121.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1162..1374
                     /gene="ybcJ"
                     /locus_tag="ACHJWB_003549"
     CDS             1162..1374
                     /gene="ybcJ"
                     /locus_tag="ACHJWB_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4315122.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1482..2003
                     /locus_tag="ACHJWB_003550"
     CDS             1482..2003
                     /locus_tag="ACHJWB_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4315123.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFDVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2039..3424)
                     /gene="cysS"
                     /locus_tag="ACHJWB_003551"
     CDS             complement(2039..3424)
                     /gene="cysS"
                     /locus_tag="ACHJWB_003551"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4315124.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3598..4092
                     /gene="ppiB"
                     /locus_tag="ACHJWB_003552"
     CDS             3598..4092
                     /gene="ppiB"
                     /locus_tag="ACHJWB_003552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4315125.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4095..4817
                     /gene="lpxH"
                     /locus_tag="ACHJWB_003553"
     CDS             4095..4817
                     /gene="lpxH"
                     /locus_tag="ACHJWB_003553"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4315126.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKLFARESGMTLLPEEKV
                     LELYGRQVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4935..5444
                     /gene="purE"
                     /locus_tag="ACHJWB_003554"
     CDS             4935..5444
                     /gene="purE"
                     /locus_tag="ACHJWB_003554"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4315127.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5441..6508
                     /gene="purK"
                     /locus_tag="ACHJWB_003555"
     CDS             5441..6508
                     /gene="purK"
                     /locus_tag="ACHJWB_003555"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4315128.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6645..7538)
                     /locus_tag="ACHJWB_003556"
     CDS             complement(6645..7538)
                     /locus_tag="ACHJWB_003556"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4315129.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFSKVDGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7535..8350)
                     /locus_tag="ACHJWB_003557"
     CDS             complement(7535..8350)
                     /locus_tag="ACHJWB_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4315130.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSYPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8361..9620)
                     /locus_tag="ACHJWB_003558"
     CDS             complement(8361..9620)
                     /locus_tag="ACHJWB_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4315131.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVSPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9630..11297)
                     /locus_tag="ACHJWB_003559"
     CDS             complement(9630..11297)
                     /locus_tag="ACHJWB_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4315132.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIYPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2551 a         3248 c         3007 g         2609 t
ORIGIN      
        1 aatatagacc tgaagcggta aattaccagg ctgaaaattc tttttatatt gtcaggtatt
       61 tcttaaatta tcttaatcct taaacaagga aataaatcag ttccagattt acaacgccat
      121 catggacgaa aaatgaagct ttcaatctca gcgacctcag cgacggtgcg cctaaccttc
      181 gcaagaggtc gcttcacgcg ataaatctga aacgaaacct gacagcgcgc cccgcttctg
      241 acaaaataga cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag
      301 caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag
      361 ttcaggcgcg tattgcagcc ggactgcggg caccaggact ggccgttgtg ctggtgggta
      421 gtaaccctgc atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt
      481 tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg ctggagctta
      541 tcgatacgct gaatgccgac aacaccatcg atggcattct ggttcaactg ccgttaccgg
      601 cgggtattga taacgtcaaa gtgctggaac gtattcatcc ggacaaagac gtggacggtt
      661 tccatcctta caacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc
      721 cgcgcggtat cgtcacgctg cttgagcgtt acaacattga taccttcggc ctcaacgccg
      781 tggtgattgg cgcatcgaat atcgtcggtc gcccaatgag catggaactg ttgctcgcag
      841 gttgcaccac taccgtgact caccgcttca ctaaaaatct gcgtcatcac gtagaaaatg
      901 ccgatctgtt gatcgttgcc gttggcaagc caggctttat tcccggtgac tggatcaaag
      961 aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg
     1021 acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt cccggcggcg
     1081 ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc
     1141 atgatccaca ggatgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag
     1201 ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg
     1261 attgccgaag gccaggtgaa agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc
     1321 gtcgccggtc agacagtgag ttttgcaggt cacagcgtac aggttgttgc ctgatccctt
     1381 tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc
     1441 atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc
     1501 acgccgccgt tcccctatgc attggtttag ggttgggttc gaaagtcatc cctccgcgtc
     1561 tgttatttgc cggaatcatc ctggcgatgc tgcccgacgc cgacgtattg tcgtttaaat
     1621 ttgacgttgc ttacggcaat gtttttggtc atcgcgggtt tacccattcg ctggtgtttg
     1681 cgtttgttgt cccgctgtta tgtgtgctga ttggacgccg atggttcagg gctgggctga
     1741 ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttgctg gattcggtaa
     1801 ccactggcgg taagggcgtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc
     1861 cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccacg ccgtacgggc
     1921 atcaggtgat aatttctgaa ttgatgtggg tgtggttgcc ggggatggtg cttatgggaa
     1981 tgttgtggtg gcgcaggcga taaccggatg cgaaaactcg catccggcaa cagcgcaatt
     2041 acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa
     2101 gacggtcacg cgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct
     2161 gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca
     2221 ggaacgcttc cggttcttgc tccagcaggc ccaatacagc ggaaagttta cgcaggtgag
     2281 atgccattgc attcgccgct gccatatctt ctgctttcag acggtttact tcacgcgcca
     2341 tatcaaacag tacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa
     2401 agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg
     2461 cagtgtagag acgctccagc gccgcacgcg cctgcttcag gttctcttcg ctgtagttca
     2521 actggctgcg atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt
     2581 tcagcacatc gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa
     2641 ccatcaccat ccccgagtgc atccagtagt tcacatactg gccatcatgg gcacaggtgg
     2701 actgcgcgat ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat
     2761 caaagtggtt acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc cagcctggac
     2821 ggcccgcgcc ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa
     2881 cgaagtccat cgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcacct gcctgcagct
     2941 ggtcgagatc ctgacgcgac agcacgccat atgtcggatc ggtcgggacg tcgaacatca
     3001 cgtcgccgtt gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa
     3061 taatttctgc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag
     3121 catcaaaatc tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc
     3181 cattttcatt ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact
     3241 tcagtttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac
     3301 gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacatac atgccgactt
     3361 ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta
     3421 gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt
     3481 cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca
     3541 ccctatatat gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg
     3601 gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct
     3661 gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc
     3721 caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa
     3781 aaagccacca aagaaccgat caaaaacgaa gccaacaacg gtctgaaaaa tacccgtggt
     3841 acgctggcaa tggcccgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac
     3901 gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc
     3961 gtgtttgctg aagtggttga aggcatggac gtggtagaca aaatcaaagg tgttgcaacc
     4021 ggtcgtagcg gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc
     4081 gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac
     4141 cggcgatcac cgccggtttt ctgcgttttt tagcggggga agcccgcaag gccgacgcgc
     4201 tgtatattct tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc
     4261 atcgccagat ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca
     4321 ttcatggcaa ccgtgatttt ctgctcggca aactctttgc ccgtgaaagc ggcatgacat
     4381 tattgccgga agaaaaggtg ctcgaacttt atggtcgcca ggtgttgatt atgcatggcg
     4441 acacgctgtg caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct
     4501 ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa
     4561 tgcgagcgaa cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc
     4621 aaaacgcggt agtcagtgcg atggaaaaac atcaggtgca atggctgatc cacggacata
     4681 cccatcgccc ggcggtacat gaacttatcg ccaaccagca acctgctttt cgcgtggtac
     4741 tcggtgcctg gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctga
     4801 ttcattttcc gttttaaaaa acccgcaact ttgctgattt cacagccacg caaccgtttt
     4861 ccttgctctc tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag
     4921 gagttttaag acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt
     4981 ccaaaagcga ctgggctacc atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc
     5041 cgcaccacgt tgaagtggtt tctgctcacc gcacccccga taaactgttc agcttcgccg
     5101 aaagcgccga agagaacggt tatcaggtga ttattgcggg cgcaggtggc gcagcgcacc
     5161 tgccaggcat gattgccgcc aaaacgctgg tgccggtgct gggcgtgcca gtacagagcg
     5221 ccgcactgag cggtgtcgat agcctctact ccatcgtaca aatgccgcgc ggcattccgg
     5281 tgggtacgct ggcgattggt aaagctggcg cggcaaacgc ggcattactg gcagcacaaa
     5341 ttctcgcgac tcacgataaa gagctacacc agcgtctgaa tgactggcgc aaagcccaga
     5401 ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct
     5461 cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt
     5521 ctggccggtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac
     5581 cgctgagatc gaacgctggc cggaaaccgc attaacccgc gagctggcgc gtcatccggc
     5641 ctttgtgaac cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga agcagctttt
     5701 cgataagctc cacctgccga ccgcgccgtg gcagttactt gccgatcgca gcgagtggcc
     5761 tgcggtgttt gatcgtttag gtgaactggc gattgttaag cgtcgcactg gtggctatga
     5821 cggtcgcggt caatggcgtt tacgcgccaa tgaaaccgaa cagttaccgg cagagtgtta
     5881 cggcgaatgt attgtcgagc agggcattaa cttctctggt gaagtgtcgc tggttggcgc
     5941 gcgcggcttt gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg
     6001 tattttgcgc accagcgtcg cttttccgca ggccaacgca cagcagcagg cgcaagccga
     6061 agagatgctg tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg
     6121 ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggct ccgcgtgtgc ataacagcgg
     6181 tcactggaca caaaacggtg ccagcatcag ccagtttgag ctgcatctgc gggcgattac
     6241 cgatctgccg ttaccgcaac cagtagtgaa taatccgtcg gtgatgatca atctgattgg
     6301 tagcgatgcg aattatgact ggctgaaatt gccgctggtg catctgcact ggtacgacaa
     6361 agaagtccgt ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg
     6421 tctgactgcg acgctggaag ccttaatccc gctgctgccg ccagagtatg ccagcggcgt
     6481 gatttgggcg cagagtaagt tcagttaagc aggtgctcat ttgattgccg gatgcggcgt
     6541 aaacgcctta tccggcttac cgctccggta cccattgtag gcctgataag acgcgtcaag
     6601 cgtcgcatca ggcatggtgc accgattgcc ggatgctctc atcactacag cgaaatacag
     6661 gtccccgctt cgcccgccag cgtctcttca attcgcgata acgccccaat ccacgcgggt
     6721 ttaccacggc ttctgacata accactcacc gccgttacct tcggccccat cgaaccatcg
     6781 actttggaaa atggcgctaa ctcatccggt gtggcatggc gaatggcgcg ttgctgcggc
     6841 gttccccagt tttcatatac cgcatcagca tcagtgagga tcaccagccc atcggcatta
     6901 atctgctcgg cgagcaacgc agcggcgaga tctttatcaa tcactgcttc actccctgcc
     6961 ccgtcttccg tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg
     7021 agcaacaact cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc
     7081 cggcgcaagt atttaccatc acgtttcatc tgccagccgt aagctgcttc cagtgcctct
     7141 tgttcttctg gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca
     7201 tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtaacag gcggcatctg cggctgtgcg
     7261 ctcaagctct gcgccagcat atagccaatc atcccctggc tttccgccac cagcacatcc
     7321 agcggatacg gttctacctc tttccacgcc agattctgta acgccagcag ccccacctgc
     7381 ggcccgttgc cgtgaacaat cgccagccga taagaacggg ccagacgtgc cagcgcgggt
     7441 acagcactgg cgatattgcg atattgattt tctgccgtca gcgcctcacc gcgctggagt
     7501 aaggcgttgc ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt
     7561 gaccaagcca gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg
     7621 aatcaatcgc aacagcggta cgtttcgggt aactcagagc atgaacaaag tgcaggagtg
     7681 gcgaggcgaa atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa
     7741 cgagttgaag attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca tctaaagcgc
     7801 cataatacca ggccgccagc agcattccgc tcaacgtgtc atcatgactt ggcgttaatc
     7861 cggggccttt acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa
     7921 aacagtgacg aaactggcgt aattcagcgg gcagcggatc gcttgccgcc atcgccagtg
     7981 gtccgaaaag tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat
     8041 gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc
     8101 cgaggcgaat cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata
     8161 acgcatcgaa ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac
     8221 gatgcagcgt taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc
     8281 ccgctaaccg ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga
     8341 tgatcgtcat tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc
     8401 tgttcaaaac acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc
     8461 tctttatggg cgataccggt attgatgagt ggcgtaatgc cggtttctac cacgcgacga
     8521 atgtccagtc ctaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg
     8581 cgctcaaggt aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc
     8641 gcaccgacaa agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag
     8701 gtttcggtaa tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg
     8761 aaaaacagac cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt
     8821 acccgaatcc cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg
     8881 atcatcgcgc cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac
     8941 tgatcggtca cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg
     9001 cgagcaattt gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga
     9061 tggaactcat cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc
     9121 tccatgcgcc ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg
     9181 cgagtcagga catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg
     9241 aggttgcagt acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg
     9301 gcagaagtaa taccgcccat tggactgacg gcattcacat ggtgacaagg aatgaagttc
     9361 acttccccct gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac
     9421 agacatgcgc ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc
     9481 ggcccggcgt gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt
     9541 tgcacatcca gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct
     9601 tgcgccactg atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagt
     9661 tttttattgc cgccggcgac tggcgaccat tggtaatgca caaccggttt gctggcgctt
     9721 tgtagctcca gcgcaaagct gcgtaatccg atgttaatca cggcgacgtt ttccagtaat
     9781 gacggtgtat gttgctgtgc ggcaggcgag agcggataaa ttaacgctgc cgccagcaag
     9841 gtggcttccg gcagcgaact cactaccgcg atccccgcat cttccagcgt ggcgatttgc
     9901 tgcgagcggc attgcgggtc acgttcagtg cctgtcaccg tggcaatggc atacagtggt
     9961 tgattatctg aacgcgcggc acaggctttt tgccaggcgc ttaccagcga ggcggcagga
    10021 tcggcagtcg caccgaagcc aatcacgaca tcaagtagca atacgcgcac ttgtggttta
    10081 gcgccgagat cggcaattaa ctggttgcgt aaggctgggt cgatcatcgg atggggacgc
    10141 ccgacggtgt agaaatcatc gccgaggtca ataatctggt gaccatcggc gtcgagcatc
    10201 atgccgtgat gatgggtatc gtcggcttcc acgccaaggt gtccagcaag taatcccgcc
    10261 gcttcggcag ccagcgtacc gccggtatac aaaccgcaaa taaatccgct gctgacaggc
    10321 gctattgcgt tacgtcgcgc cgtgacgcgt gaaagcaggc aagccaggcg tgcggcctca
    10381 tccagcgagg aggcaaacca gacattctcg tcgcgggcca ccgccggggt ataacctaaa
    10441 aacagcgcca ccgtcggttt gccggttgct ttcatggcat taacaatttt cagacgcaca
    10501 gcttcggcag gtggctttga aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc
    10561 atttccagcg ccgtcagcgc actgatgccg cctacttcac ggctgaggtc gcgcccgcca
    10621 agaccaatcg cgtgagtaat tccctcccct gcctgcgcaa tctgcgaaca cagttcctga
    10681 atcccggtac cggaagcgcc aatgacgcca atgttgcctt ccggcatcac gttagcaaaa
    10741 gccagcggcg tgccggcaat catcgacgta ccgcagtccg gccccatcac cagcaagcct
    10801 ttttctcgcg cgcgggtttt aagttggatt tcatcttcca gcgtgacgtt atcggagaac
    10861 atcatcacgt tgaggttgcg atccagcgcc tgattcgcca gctccgccgc atactcgcca
    10921 gccactgaaa tcagcgccag actggcatcg ggtaatttct gacaggcgct gtcccagcga
    10981 cgcacctgcg tcaacgcctg gctgctgcct gacccctgcg ccagttgttt tagcgcctct
    11041 tcaagctgct gcataatcgc ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc
    11101 acgcaaatat cgttcggcgt ggcgttatta aaatcgtcat gccagaaacc tgtggtatct
    11161 aataacgctt tattggcggg cgtacccatc attacggaaa catcatcaac attttctgat
    11221 tcgctgagtt ttcgtgaaat aatcattaaa ctgaccgaat cctgaaaaca ccctttttta
    11281 ataaaggcgt ggatcataac aactccttaa aaattgcacc catggcaatt gcacgagcag
    11341 tgttaatgtg gcgattacgg tatcgatgac aacgggtaaa taaggtgatg aatatcacac
    11401 agggattatt aacaa
//
LOCUS       DAZFAH010000146        11211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFAH010000146 DAZFAH010000000
VERSION     DAZFAH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.147"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..126
                     /locus_tag="ACHJWB_003560"
     CDS             <1..126
                     /locus_tag="ACHJWB_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4315133.1"
                     /translation="YLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            105..209
                     /gene="speFL"
                     /locus_tag="ACHJWB_003561"
     CDS             105..209
                     /gene="speFL"
                     /locus_tag="ACHJWB_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4315134.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            520..2718
                     /gene="speF"
                     /locus_tag="ACHJWB_003562"
     CDS             520..2718
                     /gene="speF"
                     /locus_tag="ACHJWB_003562"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4315135.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2715..4034
                     /gene="potE"
                     /locus_tag="ACHJWB_003563"
     CDS             2715..4034
                     /gene="potE"
                     /locus_tag="ACHJWB_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4315136.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4090..5730)
                     /gene="pgm"
                     /locus_tag="ACHJWB_003564"
     CDS             complement(4090..5730)
                     /gene="pgm"
                     /locus_tag="ACHJWB_003564"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4315137.1"
                     /translation="MAIHSRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAAHHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5756..6301)
                     /gene="seqA"
                     /locus_tag="ACHJWB_003565"
     CDS             complement(5756..6301)
                     /gene="seqA"
                     /locus_tag="ACHJWB_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4315138.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6486..7250
                     /gene="ybfF"
                     /locus_tag="ACHJWB_003566"
     CDS             6486..7250
                     /gene="ybfF"
                     /locus_tag="ACHJWB_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4315139.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPRARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7390..7683
                     /gene="ybfE"
                     /locus_tag="ACHJWB_003567"
     CDS             7390..7683
                     /gene="ybfE"
                     /locus_tag="ACHJWB_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4315140.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7823..8353
                     /gene="fldA"
                     /locus_tag="ACHJWB_003568"
     CDS             7823..8353
                     /gene="fldA"
                     /locus_tag="ACHJWB_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4315141.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8563..8649
                     /gene="uof"
                     /locus_tag="ACHJWB_003569"
     CDS             8563..8649
                     /gene="uof"
                     /locus_tag="ACHJWB_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4315142.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8642..9088
                     /gene="fur"
                     /locus_tag="ACHJWB_003570"
     CDS             8642..9088
                     /gene="fur"
                     /locus_tag="ACHJWB_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4315143.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9173..9499)
                     /gene="chiQ"
                     /locus_tag="ACHJWB_003571"
     CDS             complement(9173..9499)
                     /gene="chiQ"
                     /locus_tag="ACHJWB_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4315144.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9549..10955)
                     /gene="chiP"
                     /locus_tag="ACHJWB_003572"
     CDS             complement(9549..10955)
                     /gene="chiP"
                     /locus_tag="ACHJWB_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO4315145.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2714 a         2763 c         2806 g         2928 t
ORIGIN      
        1 tatttggggt atgatcgttt tttgttgatc ttcttcacag attatagcca tttcatggat
       61 agaataactc taccttcaac tgacacggca agaggtaaag gtaaatggaa aataataacc
      121 gcttaatgcc tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt
      181 ttgactttca tttctttaat gcccgctagt cttctgacta aagggcaccc caacgtacag
      241 gtctccctga ctttaagcat tacaggttaa tacctgtatt cctcggtgct catatactgc
      301 taaccctttt aaactctaaa taattcgagt cgcagcactt gcaacttgag gtatgacgag
      361 tatagccagt taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt
      421 atttatcagc ggtggagctt tgcttttttt ccggcgtgat cgatttctcc tttgagaaat
      481 tgaggacctg ctattacctg aaataaagag atgaataaaa tgtcagaatt aaaaattgcc
      541 gttagccgtt cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa
      601 agtaattata ttgacgttgc cgccattatt ttatcagtca atgatgttga acgtggaaaa
      661 ctcgatgaaa tagacgctac tggctatggc attcctgttt ttattgcaac ggaaaatgaa
      721 gaacgtgtac cagcagaata tctgccacgt atttctggtg tcttcgagca ttgcgaatca
      781 cggaaagagt tttatggacg tcagttagaa accgctgcta gccattatga aacgcaactg
      841 cgcccgccct tcttccgcgc actggtcgat tatgtcaatc agggcaatag cgcttttgac
      901 tgcccgggtc atcagggagg tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg
      961 gagtacttcg gagaaacgct gttccgttcc gacttatgca acgcagacgt agcaatgggc
     1021 gatctgctga ttcacgaagg cgcgccatgc attgcacagc aacatgcggc aaaagtgttt
     1081 aatgccgata aaacatactt cgttctaaat ggcacttcat cttctaacaa agtggtttta
     1141 aacgccctgc taacaccggg tgatctggtg ctgtttgatc gcaataacca caaatctaac
     1201 caccacggag cgttgctaca ggctggtgca acaccggttt atctggaaac ggcacgtaac
     1261 ccgtatggct ttatcggtgg cattgatgcg cactgttttg aagaaagtta cctgcgtgag
     1321 ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc gtcctttccg cctcgctgtg
     1381 attcagttag gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt
     1441 ggtcatctgt gtgactacat tctgtttgac tccgcatggg ttggctatga gcaatttatc
     1501 ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt
     1561 ctggttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat
     1621 aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac
     1681 gcctttatga tgcatgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc
     1741 aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat
     1801 ggcattaatg cccgcaagct gatcctcgat aactgtcagc atattcgtcc gttcgtacct
     1861 gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg
     1921 cgcttcttcc agtttgtacc aggtgagcac tggcattctt ttgaaggcta tgcagagaat
     1981 caatactttg tcgatccatg caaactgttg ctgacaaccc caggtattga tgcacgtaac
     2041 ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat
     2101 ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct tcctgctgac tccggcagaa
     2161 gatatggcca aacttcagca acttgttgcc ctgctggtac gcttcgaaaa actgcttgag
     2221 tccgacgcgc cattagcaga agtgctgcct tccatctaca aacagcatga agagcgctac
     2281 gccggttata cccttcgtca gttgtgccag gaaatgcatg atttgtatgc ccgtcacaac
     2341 gtgaaacaac tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt aagcatgaat
     2401 ccacaagaag ccaactacgc ctatttacgc ggtgaagtgg agctggttcg tctgccggat
     2461 gccgaaggcc gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt gctgtgcgtt
     2521 gttccgggtg aaatctgggg tggtgctgtt ctgcgttact tcagcgctct ggaagaaggg
     2581 atcaacctgt tgccaggttt tgcaccggag cttcagggtg tctatatcga agaacatgat
     2641 ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg
     2701 aaaggggaaa aattatgagt caggctaaat cgaacaaaat gggcgtcgtt cagttaacca
     2761 tactgacgat ggtcaacatg atgggctctg gcatcatcat gctgccgaca aagcttgccg
     2821 aagtcgggac aatctcaatt atctcctggc tggtgacagc cgtaggctca atggcactgg
     2881 catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc aggcggtatg ggcggttatg
     2941 cggagtatgc tttcggtaaa tccgggaact ttatggcgaa ctacacctac ggtgtctcgc
     3001 tgctgattgc taacgtcgcg attgctattt cggcggttgg ttacggcacc gaactgctcg
     3061 gcgcgagttt gtcaccagtg cagattggtc ttgcgaccat cggggtgctg tggatttgta
     3121 ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg
     3181 gggtcattat tccggtggta ggcctgtgcg ttatcggctg gttctggttt agcccgacgc
     3241 tttacgttga ttcctggaat ccgcatcatg caccgttctt cagtgcggta ggttcttcca
     3301 tcgctatgac gctatgggct tttcttggtc tggagtctgc gtgtgcgaat actgatgtag
     3361 tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg
     3421 tgatttatat cgtctccacc aacgtgattg ccgggattgt gccaaatatg gagctggcaa
     3481 attcaacggc accatttggt ctggccttcg cgcagatgtt cacgccggaa gtgggtaaag
     3541 tcattatggc gctgatggtg atgtcctgct gcggttcgct gcttggctgg cagttcacca
     3601 ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccgtg
     3661 taaccaaagt ggatgcaccg gttcagggaa tgttgaccat tgtgattatt cagagtggat
     3721 tggcactgat gaccattagc ccgtcactga acagccagtt caacgtgctg gttaacctgg
     3781 ccgtggtgac caatatcatc ccgtatattc tgtcgatggc ggcattagtc attattcaga
     3841 aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg
     3901 cgatgtatag cttttatgcg ctgtactcat ccggggaaga agccatgctg tacggttcca
     3961 tcgtgacctt ccttggttgg acactgtatg gtctggtatc accacgcttt gaactgaaaa
     4021 ataaacacgg ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat
     4081 taaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
     4141 ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt
     4201 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccaataga
     4261 agcaccgttg cccggagcag cagtcaggcg cgcggtgatc gggtcacctg ccagggtgct
     4321 ggcgctcacc atttccggag acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc
     4381 tgcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc
     4441 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca taatgatgcc
     4501 gtctttgtcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc
     4561 ttcgccgcca aagccgaagc tgccgtcgaa cagaccatca acaaaccatt tgaaacctac
     4621 cggtacttct accagcttac ggcccaagtc attgaccaca cggtcgatca tcgcagaaga
     4681 aaccagcgtt ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta
     4741 gttgatcgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
     4801 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
     4861 cagacccgcc atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag
     4921 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
     4981 atactcgcca atacgcttcc agtattcgat accggaaccg cccagcggat caacgcccag
     5041 cgtcaggccc gctttctgaa ttgccgccat atcaacgata tcggccagac cttccacaaa
     5101 cggctgcacc agatcctgct cttttacatg accggatgcc atcgcttcgt cgagggagat
     5161 acgcttcacg cctttcaggc catcggccat cagtgcgttg gccctgtctt ccaccacttt
     5221 ggtgacgttg gtatcagccg ggccaccatt tggcgggttg tacttgatac caccatcttc
     5281 cggcgggtta tgggacggtg taatcacgat accgtctgcc agcgggccac cttttttatt
     5341 gtgaaccagg atggcattgg aaatggcagg tgtcggagtg aagccattgt tttcctgcac
     5401 aatgacatca acgccgttcg ctgccagcac ttccagtacg gaaatgaacg caggctcgga
     5461 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
     5521 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggtgcgctgc
     5581 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
     5641 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
     5701 aggttggcct gcacgactgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
     5761 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
     5821 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg
     5881 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
     5941 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
     6001 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt
     6061 attcatccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg
     6121 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
     6181 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
     6241 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
     6301 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
     6361 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
     6421 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
     6481 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
     6541 tcgttcttgt ccatggtctg tttggtagcc tcgacaacct tggcgtactg gctcgcgatc
     6601 tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag
     6661 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgataccctg gatgcactgc
     6721 agatcgacaa agcaacattt atcggtcact ccatgggcgg taaagcggta atggcactta
     6781 ctgcactggc ccccgatcgg attgacaagc tggtggcaat tgatattgct ccggtcgatt
     6841 atcacgttcg ccggcatgat gagattttcg cggccatcaa cgcggtcagt gaatcggacg
     6901 cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga
     6961 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt
     7021 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc
     7081 tgtttatccc tggcggcaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
     7141 cacaatttcc acgggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
     7201 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
     7261 taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg
     7321 atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc
     7381 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
     7441 cgtcgaccgg gacgaccgaa aactaatccg ctttcgcgcg atgaacagct gcgtattaat
     7501 aaacgcaacc agctaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg
     7561 aacgcggaag ctgtcgaggc gctgaacgag ctggcggaat cgcgcaatat gagccgtagc
     7621 gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
     7681 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
     7741 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
     7801 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
     7861 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
     7921 ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg
     7981 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
     8041 cgaagagatt gatttcaatg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga
     8101 ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg
     8161 tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct
     8221 ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac
     8281 cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat
     8341 tcttaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
     8401 caatcaaaat aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg
     8461 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
     8521 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
     8581 agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg
     8641 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt
     8701 aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
     8761 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
     8821 ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
     8881 tgaactgaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gcaaggttat
     8941 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat
     9001 tcgcctgact aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga
     9061 agatgaacat gcgcacgaag gcaaataagc cagcctgaat gagaaaaagc caacctgcgg
     9121 gttggctttt ttatgcaagg gaaataagaa aagcgagagt tacagctctc acttatttgt
     9181 tattactgcg aatttcctgc caaactttat cacaatcggc tttcaccgcc tgatcatttc
     9241 cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt tcctggtcgg
     9301 caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg caggcttcaa
     9361 ttttttctgg tgaaccttgc gcggtgttga tacaggcgct gtatgcctct ttcagacggc
     9421 tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc atcatggcga
     9481 tgagaatcag ttttttcatt gaaaattcct caaacgtgcg gcaggcacaa aacctgccgc
     9541 gtcgggcatc agaagatggt gaatggtgcg attaccataa atttcacgtc acgctcatcc
     9601 tggaagatgt taccgtaacc gccgccccag cttgggatgt cggagtggtt gtcgtattcg
     9661 gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca
     9721 tccaggctgt atgcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg
     9781 ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag
     9841 cctggaagat tccagttttt caggtcatac atcgcaccga agaagaccgc tttttcgccg
     9901 ttagcgttga agtcggaacg gttatcccac cagatatcca ggcggccgtt ggaggaagcg
     9961 taggttggag tcatacgttg caggaagtat ccctgctgac cgtcagcctt aacccaggtg
    10021 ccttcgaggc gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc
    10081 caggcggtgc cgtcataaag gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca
    10141 tagaactggt agctggtggt taacgggcta ccggcgatat caaatttgta gctggctttg
    10201 gcaaaatatt gatcgatata cccttccgcc tgaccaaatg ccgcttccag tacgaagtta
    10261 tttttgaagt cgtatttcgc cccaagggag tgcagataat caactttggt ggttttatcg
    10321 ttctgataaa actcatccat ttccagatgc cacggcgctt tgtattcgtt ggtccacatg
    10381 taggagaaac tcaaggcacc agcatcgccg taatcaaaat tcgccccggc ttccgccccc
    10441 tgataagtac ctggcataaa gctccagtgc ggcgctaaca gcgtttgacc agttggctga
    10501 atgtaacctg ccctcgccca aaccggaccg tatttaaatt tggccgcagc tttatacagg
    10561 cttataccgc ttttgtcgcc ggaccagtct tcgtcatagg ctttattact ttttgaaaac
    10621 gcgatttcgt tcgggtggga gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca
    10681 gcaatatcaa ggccgaacat atcagcagca taaccggact gaaaatcgag gttggcattc
    10741 caggtggagt gagaaaggtt ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg
    10801 cgttcacgct gccagtaata tataccgccg gttaaggttg aatcgtcgat gaatccttcg
    10861 gcgcgagcct ccggcattgc cataaagccc gacattgctg taacaccggc gatagccagc
    10921 gccagcgtac tacgtttgcc actaaacgta cgcatgggtt aatcctcttt gacgtataaa
    10981 ttgctgcgcc aaaggtgaac agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    11041 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    11101 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    11161 agtttgcggt tgaaaggctt gcagaagaat aaaagcgtga atttccagca a
//
LOCUS       DAZFAH010000147        11183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFAH010000147 DAZFAH010000000
VERSION     DAZFAH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.148"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..1592
                     /gene="garD"
                     /locus_tag="ACHJWB_003573"
     CDS             21..1592
                     /gene="garD"
                     /locus_tag="ACHJWB_003573"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4315146.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     YPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1741..2076
                     /gene="prlF"
                     /locus_tag="ACHJWB_003574"
     CDS             1741..2076
                     /gene="prlF"
                     /locus_tag="ACHJWB_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4315147.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2076..2540
                     /gene="yhaV"
                     /locus_tag="ACHJWB_003575"
     CDS             2076..2540
                     /gene="yhaV"
                     /locus_tag="ACHJWB_003575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4315148.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLVVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2595..3404)
                     /gene="agaR"
                     /locus_tag="ACHJWB_003576"
     CDS             complement(2595..3404)
                     /gene="agaR"
                     /locus_tag="ACHJWB_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4315149.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3653..4933
                     /gene="kbaZ"
                     /locus_tag="ACHJWB_003577"
     CDS             3653..4933
                     /gene="kbaZ"
                     /locus_tag="ACHJWB_003577"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4315150.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPCVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            4956..5429
                     /gene="agaV"
                     /locus_tag="ACHJWB_003578"
     CDS             4956..5429
                     /gene="agaV"
                     /locus_tag="ACHJWB_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4315151.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5440..6219
                     /gene="agaW"
                     /locus_tag="ACHJWB_003579"
     CDS             5440..6219
                     /gene="agaW"
                     /locus_tag="ACHJWB_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4315152.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6209..7087
                     /gene="agaE"
                     /locus_tag="ACHJWB_003580"
     CDS             6209..7087
                     /gene="agaE"
                     /locus_tag="ACHJWB_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4315153.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7105..7539
                     /gene="agaF"
                     /locus_tag="ACHJWB_003581"
     CDS             7105..7539
                     /gene="agaF"
                     /locus_tag="ACHJWB_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4315154.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7536..8669
                     /gene="nagA"
                     /locus_tag="ACHJWB_003582"
     CDS             7536..8669
                     /gene="nagA"
                     /locus_tag="ACHJWB_003582"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4315155.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9020..10174
                     /gene="agaS"
                     /locus_tag="ACHJWB_003583"
     CDS             9020..10174
                     /gene="agaS"
                     /locus_tag="ACHJWB_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4315156.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10187..11047
                     /gene="kbaY"
                     /locus_tag="ACHJWB_003584"
     CDS             10187..11047
                     /gene="kbaY"
                     /locus_tag="ACHJWB_003584"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4315157.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2691 a         2891 c         2966 g         2635 t
ORIGIN      
        1 tggctggaga atattgcaca atggccaaca tcgaaatcag acaagaaacg ccaactgcgt
       61 tttatataaa agttcacgac acagataatg tggcaattat tgttaatgat aatggcctga
      121 aagcaggaac gcgttttccg gatgggctgg aattaattga acatattccc caggggcata
      181 aagtcgcatt gctggacatt ccggctaatg gtgaaattat tcgttatggc gaagtgattg
      241 gttacgccgt gcgcgcaatc ccacgcggaa gctggatcga cgaatcaatg gttgtactac
      301 cggaagcgcc gccgttacac acgctgccac tggcaaccaa agtcccggaa cccttaccgc
      361 cgctggaagg ttacaccttt gaaggctatc gcaatgccga tggcagcgtg ggcaccaaaa
      421 acctgctcgg catcaccacc agcgtccact gtgtggcagg cgtggtggac tacgtagtaa
      481 aaatcattga acgcgatctg ctgccgaaat acccgaacgt cgatggcgtg gtggggctga
      541 atcatctgta cggttgtggc gtggcgatta acgctccggc ggcggtggtg cccattcgga
      601 ccattcacaa catttcgctt aacccaaact ttggcggcga ggtgatggtg attggcctgg
      661 gttgcgagaa attacagcca gaacgcctgc tggtcggcac ggatgatgta caaggtattc
      721 cgctggagaa cgccagtatt gtcagcttgc aggatgaaaa gcatgtcggt tttcagtcca
      781 tggtcgagga tattttgcag atcgccgaac gccatctaca aaaactgaat caacggcagc
      841 gagaaaccta cccggcttca gaactggtcg ttggtatgca gtgcggtggc agcgatgcgt
      901 tttctggtgt aacggcaaac ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg
      961 gcgcaacggt gatgttttca gaagtaacgg aagtgcgtga cgcgatccat ctgctgacac
     1021 cacgcgcagt gaacgaagag gtcggcaaac ggctgctgga ggagatggag tggtacgata
     1081 actatctcaa tatgggaaaa accgaccgca gcgccaaccc ttcgccgggc aacaagaaag
     1141 gcggtctggc aaacgtggta gagaaggcac tcggctccat tgctaaatcg ggtaaaagcg
     1201 caattgttga agtgctgtcg cccggtcaac gcccgactaa acgcggatta atttacgccg
     1261 cgacgccagc cagcgatttt gtctgtggca cgcaacaggt ggcttcgggt atcacagtgc
     1321 aagtgtttac gaccggtcgt ggtacgccgt acggcctgat ggcggtaccc gtcattaaaa
     1381 tggcaacccg caccgagctg gcgaaccgct ggtttgattt aatggatatt aatgcgggca
     1441 ccatcgctac cggcgaagaa actattgaag aggtgggctg gaagttgttc cactttattc
     1501 tcgacgtcgc cagcgggaag aagaaaacct tctcggatca atggggactg cataaccagc
     1561 tggcggtgtt taacccggca ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca
     1621 tggccttagt gccatgtttt tattgtttaa agcccccacg tccattaata atgcatttgc
     1681 attaccttag ttcaagctta taatttgagc agaaaacagg ctgtaaaagg acagtgaatc
     1741 atgcccgcta atgctcgctc tcacgctgta ctgaccactg aatcaaaggt cacgatacgc
     1801 ggacaaacaa ctatccccgc gccagtgcgt gaggccttaa aactgaagcc aggccaggac
     1861 agcattcatt acgaaattct gcctggtggg caagtcttta tgtgccgact gggagatgaa
     1921 caggaggatc atactatgaa tgcatttttg cgttttctgg atgcagatat ccagaacaac
     1981 ccgcaaaaaa ctcgtccatt caacattcaa caaggaaaga aacttgtcgc tggcatggac
     2041 gtcaacattg atgatgagat tggcgacgac gaataatgga ttttccacaa agggttaatg
     2101 gttgggcgct atatgctcat ccctgttttc aggaaaccta cgacgcttta gttgccgaag
     2161 tcgagacatt aaagggaaaa gatcctgaaa attatcagag aaaagccgcc acaaagttat
     2221 tggtggtagt ccataaagtg attgaggagc atatcacggt caatccatca tcaccggcat
     2281 tccgtcatgg caagtcgtta ggctctggga aaaataaaga ctggtcacgg gtaaaatttg
     2341 gtgctggtcg ttatcgtctc ttctttcgtt atagtgaaaa agagaaagtc atcattctgg
     2401 gatggatgaa cgatgaaaac actctgcgca cctacggtaa aaaaacagat gcctataccg
     2461 tattcagcaa aatgttaaaa agaggacatc ctcctgccga ctgggaaagc ctcacccaag
     2521 agacagaaga aaaccattga tggtgttcac aatgcccgcc gaacgtattc gtgttgacca
     2581 gcgggcaccc tttctcactc cccgaccaga atcacttcaa ccccagcctt tcgcagtcct
     2641 tccagactat ccgcaggaat gccttcatca acaatgatca tgtcgatacg ttgagtatca
     2701 atgatcttat gtaaactgga acgattgaat ttactggaat cggtgaccac gatgatccgt
     2761 tccgcaactt cgcacatccg acggtttaaa cgggcttcat cttcattatg tgtgctgacg
     2821 ccgcgctcca gatcgatcgc atctacacca agaaacagca tatcgaagtg gtaattttgc
     2881 agcgattgct cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg
     2941 gtcatcagca gctcaacgcc ttccgcttcc agcaacgcat tagccacgtt cataccgttg
     3001 gtcatcgcaa ttacgtcagt gtgcttacgc atcagacgag caatctcaaa agtggtagtc
     3061 ccggaatcga ggatcacccg atgaccaggc tgaatcaact caacggcagc tttcgcaacg
     3121 ctgcgtttca tcgcggtgtt cagcgcgctt ttatcttcca ctgatggctc gactgacggc
     3181 gtcgtgctat cgcagatcaa cgcgccacca taggcacgca cagcgatccc ctgcttttcc
     3241 agaaacgcca gatcattgcg gatcgtcaca gtagatacgc catacaatgc cgacagatcg
     3301 ttaacctgca cactcccttg ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg
     3361 gtgccagtca ctcgcttctc acctgaagcg tcggtattac tcatagtaag tcctttcgta
     3421 aaactttcgt ttcatttcgt tttgcctatt aacgcctttc tattaagcaa atgcaagccc
     3481 accttgccca ttgacgcaag ctactctcgt ttcagtgact ttcattatgt ttcttttgtg
     3541 aatcagatca gaaaactatt atctttcgtt ttatttttat ctcaccatga cgcagtatca
     3601 actgaaacaa aacgaaagat taatatcgca gtaatctgaa ctggagagga aagtgaaaca
     3661 tctgacagaa atggtgagac agcacaaagc gggcaaaaca aatggaattt atgccgtttg
     3721 ttccgcacat ccgctggtgc tggaggctgc aatccgctac gccagtgcaa accaaacgcc
     3781 gttactgatt gaagcaacct ccaatcaggt agaccagttc ggcggttata ccggaatgac
     3841 gcccgccgat tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga
     3901 tgcgttgatt ctgggtggtg accatctggg gccaaaccgc tggcaaaacc tgccggccgc
     3961 tcaggcaatg gccaatgccg atgatttgat taaaagctac gttgcggcag gattcaaaaa
     4021 aatccacctt gattgcagca tgtcctgtca ggacgatccg attcccttaa ctgatgacat
     4081 cgtggctgaa cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc ttgaacactt
     4141 tggcgaagcc gatctggagt atgtcattgg taccgaagtg ccggtacctg gcggcgcgca
     4201 tgaaacctta agcgagctgg cggtcaccac gccggatgcc gcccgcgcca cgctggaagc
     4261 ccatcgtcac gcctttgaaa agcaaggttt gaatgccatc tggccacgca tcattgccct
     4321 ggtggttcaa ccctgcgtcg aattcgatca caccaacgtt attgattatc agcccgccaa
     4381 agcgagcgcc ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc
     4441 taccgattat caaacgccgc aatcgctgcg ccagctggtg attgaccact ttgccattct
     4501 gaaagttggc ccggcgctga ccttcgccct gcgtgaagcc cttttttctc tggcggcgat
     4561 tgaagaagaa ctggtaccag cgaaagcctg ttccggtctg cgtcaggtgc tggaaaacgt
     4621 gatgctcgac cgcccggaat actggcaaag ccactaccac ggtgacggca acgcgcgtcg
     4681 tctggcgcgt ggttatagct actcggatcg cgtgcgctat tactggccgg acagccagat
     4741 tgatgacgct ttcgcccatc tggttcgtaa tctggcggat tcaccgattc cgctgccgct
     4801 catcagccag tatctgccgc tgcaatacgt gaaagttcgc tccggcgagc tgcaaccgac
     4861 gccacgggaa ctcattatca accatattca ggacatcctg gcgcagtact acacagcctg
     4921 tgaaggccaa taagcaaaac aaagaggaac acgctatgcc aaatattgtt ttaagccgga
     4981 ttgatgaacg cttgattcac ggtcaggtcg gcgttcagtg ggtcggattt gcgggggcaa
     5041 atctggtgct ggtagccaac gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg
     5101 aaatggtact ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa gttatcgaca
     5161 acattcatcg cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt
     5221 tcctgacgct ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc aatatgcact
     5281 acgccaatgg caaacaacaa atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag
     5341 catttaacga cctgaaagcc gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc
     5401 ctgctgtgga cctctttaaa ttactttgag ggattcatca tggaaatcag cctgttgcag
     5461 gcatttgcgt tgggcattat cgcctttatc gctggcctgg atatgtttaa cggcttaacc
     5521 catatgcacc gcccggtggt cctcggcccg ttggtcgggc tggtacttgg cgatctgcat
     5581 accggaattt taaccggcgg tacgctggaa ctggtgtgga tggggctggc cccgctggcg
     5641 ggcgcacagc cgcctaacgt gattatcggt actatcgtcg gcacggcgtt tgccattact
     5701 actggcgtga aacccgatgt cgcagtcggt gtcgccgtac ctttcgctgt cgcagtacag
     5761 atggggatta ccttcctgtt ctcggtgatg tccggcgtga tgtctcgctg cgaccggatg
     5821 gcggagaacg ccgatactcg cggcattgaa cgcgtgaact atctggcact gctggcactc
     5881 ggcactttct attttctctg cgctttcctg cctatctact tcggcgcgga acatgcaaaa
     5941 accatcattg atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc
     6001 atgccagcaa tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt ctacatcccc
     6061 tacttcatcc tgggctttgt tgccgcagcc tggctcaagt taccggtgct ggctattgct
     6121 gccgctgcgc tggcgatggc gctgatcgac ctgctgcgta aatctcctga accgactcaa
     6181 cctgcggcac agaaagagga atttgaagat ggcatctaat caaaccaccc tgccgaacgt
     6241 cagtgaaaac gaagaaacat tgctgactgg cgtcaatgaa aacgtgtatg aagatcagag
     6301 cattggcgcg gagctgacga aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt
     6361 acaagcgtct tttaactacg aacgtatgca ggcttctggc tggttgtacg gtctgctgcc
     6421 tgctctgaaa aagatccaca ctaataaacg cgacctggcg cgcgccatga aggggcatat
     6481 gggtttcttc aatacccatc cgtttctggt gacatttgtt atcggcatta tccttgcgat
     6541 ggagcgttct aagcaggacg ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc
     6601 gctcggcggg attggtgatg cgatgttctg gctgacgcta ctgccgattt gtggcgggat
     6661 aggcgcaagc cttgcgctac aaggctctat tcttggcgca gtcgtattta ttgtgctgtt
     6721 caacgtggta cacctggggc tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt
     6781 ggcggcgatt ccactcatta aagcgaatac caaaaaagtc ggccatgctg cgtctatcgt
     6841 tgggatgacg gtaatcggcg cgctggtggc aacctatgtt cgtttaagca ccacgctgga
     6901 aatcacagcg ggcgacgcag tggttaagtt acagactgat gttattgaca aactgatgcc
     6961 tgccttctta ccgctggtct acaccttgac catgttctgg ctggtacgcc gcggctggag
     7021 tccgctgcgc ctgattgcag ttaccgtggt tctcggcatc gtcggtaaat tctgccattt
     7081 cctttaaata caatgaggta ttagatgtta agtattattt tgacagggca tggcgggttt
     7141 gccagtggca tggaaaaagc aatgaagcaa attctcggtg agcaatcgca gtttatcgcc
     7201 atcgattttc cggaaacctc aagcaccgcg ctgcttacat cgcagcttga agaggccatc
     7261 gcccaactgg attgtgaaga cggcattgtt tttctgacgg atttgctggg cggcacgccg
     7321 tttcgcgtag cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat caccggcacc
     7381 aatttgcaac tgctactgga gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc
     7441 cgtgtacagg cgctggagtg tggacatcgt gggctgacca gtctggtgga cgagttaggc
     7501 cgctgccatg aagaatgtcc ggtcgaggaa gggatatgac acacgttctg cgcgccagaa
     7561 ggctgctgac tgaagaggga tggctcgatg accatcagtt gcgtattgct gacggtgtca
     7621 tcgcagcaat cgaaccgatt ccagtgggcg tgactgaacg cgatgcggaa ctgctctgcc
     7681 ccgcttacat cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc
     7741 cggatgtact cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc agttggttgc
     7801 cgactaccgt aaccgcgccg cttaacacca ttcatgcggc gctgaaacgt attgctcaac
     7861 gttgccaacg cggcggacct ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact
     7921 tcacgccgca gaataaaggc gcgcatccgc cggaattgtt tcgcgagctt gaaattgccg
     7981 agctggatca attgattgcc gtttctcagc acaccttacg cgtggtagcg ctggcaccgg
     8041 aaaaagaggg ggcattgcag gccatccgcc atcttaaaca gcaaaatgta cgagtgatgc
     8101 tggggcatag cgcggcgacc tggcaacaaa ctcgcgccgc gtttgatgct ggtgccgacg
     8161 gcctggtgca ttgctataac gggatgacag gtttacatca ccgcgaaccg ggaatggttg
     8221 gcgcgggatt aacggacaag cgcgcctggc tggaactgat agccgatggt catcatgtgc
     8281 atccggcggc gatgtcgctg tgttgttgct gtgcaaaaga gagaatcgtg ctgatcaccg
     8341 acgcgatgca ggcagccggg atgccggatg gtcgctatac attatgtggc gaagaagtgc
     8401 agatgcacgg tggcgttgtc cgtaccgcgt ccggtgggct ggcgggcagt acgctgtctg
     8461 ttgatgcggc agtgcgcaac atggtcgagt tgacgggcgt aacgcctgcg gaagccattc
     8521 atatggcatc gctgcatccg gcgcgaatgc tgggtgttga tggtgttctg ggatcgctta
     8581 aaccgggcaa acgcgccagc atcgttgcgc tggatagcgg gctgcatgtg caacaaatct
     8641 ggattcagag tcaattagct tcgttttgat attttgctcc tttattgggc cttcacttcc
     8701 cccgtaaggc ctttcttttt ctttcgtttt gatctgtgca gcggtgtcgg atgcgacgct
     8761 aacgcgtctt atgcgaccta cggtacgccg catccagcaa tcggtgaaca tagcgacgtc
     8821 attacgcaat tctttaatct tcccttgtct tttctttcaa tgatcacaga tttcgtttta
     8881 tttccttttc ccccattgaa ctttcggttt cttttctata gattttaatc aacgaaagac
     8941 atcaccaagt gaaatgaaac gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca
     9001 tcagactaag gattgagtta tgccagaaaa ttacacccct gctgccgccg caaccggtac
     9061 atggactgaa gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat
     9121 cgacgcgcta cgttctgcgc tcaataactt ccttgaaccg ttactgcgca aagagaatct
     9181 gcggatcatc ctgaccggag ccggaacctc ggcatttatc ggtgacatca tcgcgccgtg
     9241 gctcgccagc cataccggta aaaacttcag cgccgtaccg accaccgatc tggtcaccaa
     9301 tccgatggac tacctgaacc cagctcatcc gctgctgttg atctccttcg gtcgatccgg
     9361 caacagcccg gaaagcgtcg ctgccgtgga actggcaaat caatttgtac cagaatgcta
     9421 tcacctgccg atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga
     9481 taacgcgttt gccctgctga tgcccgcaga aacgcacgat cgcggctttg cgatgaccag
     9541 cagcattacc accatgatgg ccagctgcct cgcggttttc gcacctgaga cgatcaacag
     9601 ccaaaccttc cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt
     9661 cagcgaaggt gtgtttggtt acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg
     9721 cttacagggc gcagcacgcg agtcggcgct gaaagtgctg gaactgactg cgggtaaact
     9781 ggcggccttc tatgattccc cgaccggatt ccgtcatggc ccgaaatcac tggtcgataa
     9841 cgaaacgctg gtggtggtat ttgtctcaag ccacccttac acccgtcagt atgatcttga
     9901 tctgctggct gaactccgcc gtgacaacca ggcaatgcgc gtaatcgcca tcgccgcgga
     9961 aagcaccgac atcgtcgctg ccggtccaca tattatcctg ccgccgtcac gtcactttat
    10021 cgacgttgag caggcatttt gcttcctgat gtacgcccag acgtttgcac tgatgcagtc
    10081 gctgcacatg ggcaatacgc cggatacccc atcagccagc ggcaccgtta accgcgtggt
    10141 gcaaggcgta atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc
    10201 cactaaatat ctgttacagg acgcccaggc caatggctac gcggtgcctg cttttaacat
    10261 tcataacgcc gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt
    10321 gatcctcgcc ggaacgccgg ggacctttaa acatatcgcg ctggaagaga tctacgccct
    10381 gtgcagcgcc tattccacaa cctacaacat gccactggcg ctgcatctcg accaccacga
    10441 atcgctggat gatattcgcc gtaaagtcca cgcaggtgtg cgcagcgcaa tgatcgacgg
    10501 cagccacttc ccgtttgccg agaacgtgaa gctggtgaaa tcggttgttg acttctgcca
    10561 ctcacaggat tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg aagatgacat
    10621 gagcgttgac gccgaaagtg cattcctgac cgatccacaa gaagctaaac gctttgtcga
    10681 actgactggc gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa
    10741 aacgccgaag attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct
    10801 ggtgctacat ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg
    10861 cgtcacaaaa gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc
    10921 ctggtttgcg gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggaatgga
    10981 tgcgatgaaa gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc
    11041 agcataatca ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag
    11101 tgccttaatg gaaaaggaga tgactaaacc ttaataaata catcagtaca atatcgcaac
    11161 aataatatat taaaaaaaat tat
//
LOCUS       DAZFAH010000148        11143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFAH010000148 DAZFAH010000000
VERSION     DAZFAH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.149"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..273
                     /gene="yoaC"
                     /locus_tag="ACHJWB_003585"
     CDS             <1..273
                     /gene="yoaC"
                     /locus_tag="ACHJWB_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4315158.1"
                     /translation="DFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARA
                     DQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(393..572)
                     /gene="yoaH"
                     /locus_tag="ACHJWB_003586"
     CDS             complement(393..572)
                     /gene="yoaH"
                     /locus_tag="ACHJWB_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4315159.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            646..2007
                     /gene="pabB"
                     /locus_tag="ACHJWB_003587"
     CDS             646..2007
                     /gene="pabB"
                     /locus_tag="ACHJWB_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4315160.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2011..2589
                     /gene="nudL"
                     /locus_tag="ACHJWB_003588"
     CDS             2011..2589
                     /gene="nudL"
                     /locus_tag="ACHJWB_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4315161.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPSDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2773..4137
                     /gene="sdaA"
                     /locus_tag="ACHJWB_003589"
     CDS             2773..4137
                     /gene="sdaA"
                     /locus_tag="ACHJWB_003589"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4315162.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4268..5866
                     /gene="pdeD"
                     /locus_tag="ACHJWB_003590"
     CDS             4268..5866
                     /gene="pdeD"
                     /locus_tag="ACHJWB_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4315163.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5870..7426)
                     /gene="yoaE"
                     /locus_tag="ACHJWB_003591"
     CDS             complement(5870..7426)
                     /gene="yoaE"
                     /locus_tag="ACHJWB_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4315164.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7414..7572)
                     /gene="yoaL"
                     /locus_tag="ACHJWB_003592"
     CDS             complement(7414..7572)
                     /gene="yoaL"
                     /locus_tag="ACHJWB_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4315165.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7889..8860
                     /gene="manX"
                     /locus_tag="ACHJWB_003593"
     CDS             7889..8860
                     /gene="manX"
                     /locus_tag="ACHJWB_003593"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4315166.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8923..9723
                     /gene="manY"
                     /locus_tag="ACHJWB_003594"
     CDS             8923..9723
                     /gene="manY"
                     /locus_tag="ACHJWB_003594"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4315167.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9736..10587
                     /gene="manZ"
                     /locus_tag="ACHJWB_003595"
     CDS             9736..10587
                     /gene="manZ"
                     /locus_tag="ACHJWB_003595"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4315168.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10642..11100
                     /gene="yobD"
                     /locus_tag="ACHJWB_003596"
     CDS             10642..11100
                     /gene="yobD"
                     /locus_tag="ACHJWB_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4315169.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2724 a         2819 c         2821 g         2779 t
ORIGIN      
        1 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcaca
       61 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      121 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      181 gcaaaaaaaa tggagtctgg tgaacgcact gtaattaaaa atcctgaata cttttcaaca
      241 tatatgcagg aagaactaaa agcactggtc tgagcttaat ttatatcagc ataaatgggt
      301 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa taatcatcgg
      361 attctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      421 acgattcgct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      481 ccgctgctca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      541 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      601 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      661 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      721 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      781 tttgatattg tggtcgccga tccgatttgc actttaacca ctttcggtaa agaaaccgtt
      841 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
      901 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
      961 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1021 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1081 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1141 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1201 tccaatatga cccgcgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1261 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1321 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1381 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1441 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1501 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1561 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1621 ccagagctgt tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1681 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1741 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1801 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1861 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     1921 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     1981 aagatattac gccaactgga gaagtaagac gtggaatacc gtagcctgac gcttgatgat
     2041 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2101 caggctgccg tgttaatccc catcgtccgt cgaccgcaac cggggttgtt gctgactcag
     2161 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2221 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2281 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2341 caggtaaccc cagtggtcgg cattatcccg tccgatctgc cgtatcgcgc cagtgaagat
     2401 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2461 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2521 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2581 aaaccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2641 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2701 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2761 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2821 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa
     2881 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     2941 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3001 accgtggata tcgacagtat tcccggtttt attcgcgacg tagaagagcg cgaacgtctg
     3061 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3121 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3181 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3241 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3301 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3361 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3421 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3481 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3541 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3601 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3661 ctggcttact atgaccactt tattgaatcg gtcagcccgg acatctatac ccgttacttt
     3721 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3781 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3841 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     3901 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     3961 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4021 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4081 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4141 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4201 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4261 ggcccgcatg caaaaagcac aacggatcat taaaacctat cgccgtaatc gaatgattgt
     4321 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4381 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4441 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4501 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4561 cattggcctg gtgcaagacg gcacactcta ttgctccagc atttttggtt atcgcaatgt
     4621 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4681 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcgggcag
     4741 tagcaatgct ggggtcatgg agatgattaa catcgactta ctgacggcaa tgctccttga
     4801 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4861 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     4921 ttcgcaacgc tttcctttta ccattaacgt taatggtccg ggggctacgg cgctggcatg
     4981 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctacat
     5041 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaatcaatc tgggcctggc
     5101 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5161 tggtgtagag attttgctgc gctggaacaa tccgcgtcag ggctggattt caccggatgt
     5221 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5281 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5341 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5401 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5461 tgttgattat cggattgccc gcgagctaca tcgtaaaaac gtcaaactgg cgattgatga
     5521 cttcggcacc ggcaacagtt cattttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5581 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5641 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac
     5701 gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta
     5761 cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc
     5821 cgcccggcat aatggacata tcacgcccat tatgccgtta cgttaatgct tactcatctt
     5881 catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt
     5941 caatgatggt gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac
     6001 catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct
     6061 caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt
     6121 caccatcagt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt
     6181 ccagcacatc cagcggcgtg accagacctt gtaccacacc aaactcgttg gtcacgataa
     6241 caaagctccc gcgagcacga cgcagcacgc ccaacaggtt gatcggatcg agggtttccg
     6301 ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg
     6361 ccaccagcag ttctttagca cgtacaatac cgatgatttc atccagttca ccgcgacata
     6421 ccgggaacag actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat
     6481 tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg
     6541 ccagcgtcag tacgccgtta atcatgtaac gttcttcttc ggcaaatgca ccttccggga
     6601 tcggcatcgg catcgggtta tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca
     6661 tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac tggtggcgaa
     6721 taaagttgcg acgcgcaatc tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg
     6781 catacaggta acctttcgga atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa
     6841 tcattaacag gaagctcaga cagagcacca ccaccgtggg gtgctggtta acgaatcgcg
     6901 tcagcggttt ggatgccagc aacataaccg ccatcgcaat cactaccgcc gccatcatca
     6961 ccggcagatg gttaaccatc cctactgcag taattaccgc atccaacgag aagacggcgt
     7021 caaggatgac aatctgtgtg acgaccaccc agaaactggc gtagccttta ccgtggccgg
     7081 aatcatgatc gcggttttcc agccgttcat gcagttcggt tgttgctttg aacagcaaga
     7141 atatcccccc gaacaacata atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa
     7201 atagcggttt ggtcagcgtg accatccatg aaatcagcga cagcagcccc agacgcataa
     7261 tcagcgccag tgataacccc agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt
     7321 cagcaagaat ggcgatgaag accaggttat cgatacccag cacaatttcg agaacaacaa
     7381 gcgtgagtag ccccgcccaa attgaggggt ccattaagaa ttccatgaca agctcctgct
     7441 taaggaatag ctattcgacg ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa
     7501 ggtgcggcat agagagccag aaaggtaggc gttaaaaggc ctgatgctga aatgacgtcg
     7561 gtgacgatcc atactgcggg ctactgccct atactccatg gttgttaaac gggagttaaa
     7621 catatcagag acgcctctga tttggcaaag atttaccttc ctttgcaaac gaatgtgaca
     7681 aggatatttt acctttcgaa atttctgcta atcgaaagtt aaattacgga tcttcatcac
     7741 ataaaataat tttttcgata tctaaaataa atcgcgaaac gcaggggttt ttggttgtag
     7801 cccttatctg aatcgattcg attgtggacg acgattcaaa aatacatctg gcacgttgag
     7861 gtgttaacga taataaagga ggtagcaaat gaccattgct attgttatag gcacacatgg
     7921 ttgggctgca gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt
     7981 cggctggatc gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc
     8041 tcagttggca aaactcgaca ccactaaagg cgtgctgttt ctcgttgata catggggagg
     8101 cagcccgttc aatgctgcca gccgcattgt cgtcgacaaa gagcattatg aagtcattgc
     8161 aggcgttaac attccaatgc tcgtggaaac gttaatggcc cgtgatgatg acccaagctt
     8221 tgatgaactg gtggcactgg cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc
     8281 caaaccggtt gaaaaagccg cgccagcacc cgctgccgca gcaccaaaag cggctccaac
     8341 tccggcaaaa ccaatggggc caaacgacta catggttatt ggccttgcgc gtatcgacga
     8401 ccgtctgatt cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat
     8461 tattgttgtt agtgatgaag tggctgcgga taccgttcgt aagacactgc tcacccaggt
     8521 tgcacctccg ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa
     8581 caacccgaaa tatgctggcg aacgcgtaat gctgttattt accaacccaa cagatgtaga
     8641 gcgtctcgtt gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta tggcattccg
     8701 tcagggtaaa acccaggtga ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt
     8761 caagaaactg aatgcgcgcg gtattgagct ggaagtccgt aaggtttcca ccgatccgaa
     8821 actgaaaatg atggatctga tcagcaaaat cgataagtaa cgtattgtgt tgattatcac
     8881 tcagttttca cacttaagtc ttacgtaaac aggagaagta caatggagat taccactctt
     8941 caaattgtgc tggtatttat cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa
     9001 tttcagtttc accgtccgct aatcgcgtgt accctggtgg gtatcgttct tggggatatg
     9061 aaaaccggta ttattatcgg tggtacgctg gaaatgatcg cgctgggctg gatgaacatc
     9121 ggtgctgcag ttgcgcctga cgccgctctg gcttctatca tttctaccat tctggttatc
     9181 gcaggtcatc agagcattgg tgcaggtatc gcactggcaa tccctctggc cgctgcgggc
     9241 caggtactga ccatcatcgt tcgtactatt accgttgctt tccagcacgc tgcggataag
     9301 gctgctgata acggcaacct gacagcgatt tcctggatcc acgtttcttc tctgttcctg
     9361 caagcaatgc gtgtggcaat tcctgccgtc atcgtggcgc tgtctgttgg taccagcgaa
     9421 gtacagaaca tgctgaatgc gattccggaa gtggtgacca atggtctgaa tatcgccggt
     9481 ggcatgatcg tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg
     9541 atgccgttct tctacctcgg cttcgtaacc gcagcattca ccaactttaa cctggttgct
     9601 ctgggtgtga ttggtactgt tatggcagtg ctctacatcc aacttagccc gaaatacaac
     9661 cgcgtagccg gtgcgcctgc tcaggcagct ggtaacaacg atctcgataa cgaactggac
     9721 taacaggtga gcgaaatggt tgatacaact caaactacca ccgagaaaaa actcactcaa
     9781 agtgatattc gtggcgtatt cctgcgttct aacctcttcc agggttcatg gaacttcgaa
     9841 cgtatgcagg cactgggttt ctgcttctct atggtaccgg caattcgtcg cctctaccct
     9901 gagaacaacg aagctcgtaa acaagctatt cgccgtcacc tggagttctt taacacccag
     9961 ccgttcgtgg ctgcgccgat tctcggcgta accctggcgc tggaagaaca gcgtgctaat
    10021 ggcgcagaga tcgacgacgg tgctatcaac ggtatcaaag tcggtttgat ggggccgctg
    10081 gctggtgtag gcgacccgat cttctgggga accgtacgtc cggtatttgc agcactgggt
    10141 gccggtatcg cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat cctgtttaac
    10201 ctggtgcgtc tggcaacccg ttactacggc gtagcgtatg gttactccaa aggtatcgat
    10261 atcgttaaag atatgggtgg tggcttcctg caaaaactga cggaaggggc gtctatcctc
    10321 ggcctgtttg tcatgggagc attggttaac aagtggacac atgtcaacat cccgctggtt
    10381 gtctctcgca ttactgacca gacgggcaaa gaacacgtta ctactgtcca gactattctg
    10441 gaccagttaa tgccaggcct ggtaccactg ctgctgacct ttgcttgtat gtggctactg
    10501 cgcaaaaaag ttaacccgct gtggatcatc gttggcttct tcgtcatcgg tatcgctggt
    10561 tacgcttgcg gcctgctggg actgtaagac tgttgtacac taccggggcc ttttggcccc
    10621 gtttttttat ctggaggatt aatgacaatc acggacctgg tactgattct tttcatcgcc
    10681 gcactcctgg ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa cggccccacc
    10741 ctgctggcaa ttcctttgct ccggcgtggt cgcatcgata gcgttatctt cgtcggattg
    10801 attgtcattc ttatctataa caacgtcacg aatcatggtg cgttaataac gacatggtta
    10861 ttaagcgcac tggctctgat gggtttttat atattctgga tccgcgttcc gaagatcatc
    10921 tttaaacaaa aaggtttttt cttcgccaat gtctggattg aatatagccg aatcaaagcg
    10981 atgaacttgt cggaagatgg cgtgttggtg atgcaattag aacagcgtcg gctgttaatc
    11041 cgcgttcgaa atatcgacga tctggaaaaa atttataagc ttctcgtttc aactcaataa
    11101 gttatgaatt tagccaaagc tatgtttagt gtatttttaa taa
//
LOCUS       DAZFAH010000149        11094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFAH010000149 DAZFAH010000000
VERSION     DAZFAH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.150"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..687)
                     /gene="nrfG"
                     /locus_tag="ACHJWB_003597"
     CDS             complement(91..687)
                     /gene="nrfG"
                     /locus_tag="ACHJWB_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4315170.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(684..1067)
                     /gene="nrfF"
                     /locus_tag="ACHJWB_003598"
     CDS             complement(684..1067)
                     /gene="nrfF"
                     /locus_tag="ACHJWB_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4315171.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1060..2778)
                     /gene="nrfE"
                     /locus_tag="ACHJWB_003599"
     CDS             complement(1060..2778)
                     /gene="nrfE"
                     /locus_tag="ACHJWB_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4315172.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(2798..3754)
                     /gene="nrfD"
                     /locus_tag="ACHJWB_003600"
     CDS             complement(2798..3754)
                     /gene="nrfD"
                     /locus_tag="ACHJWB_003600"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4315173.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3751..4422)
                     /gene="nrfC"
                     /locus_tag="ACHJWB_003601"
     CDS             complement(3751..4422)
                     /gene="nrfC"
                     /locus_tag="ACHJWB_003601"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4315174.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRVFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLAQGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4419..4985)
                     /gene="nrfB"
                     /locus_tag="ACHJWB_003602"
     CDS             complement(4419..4985)
                     /gene="nrfB"
                     /locus_tag="ACHJWB_003602"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4315175.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(5030..6466)
                     /gene="nrfA"
                     /locus_tag="ACHJWB_003603"
     CDS             complement(5030..6466)
                     /gene="nrfA"
                     /locus_tag="ACHJWB_003603"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4315176.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWDEQARKNGLLSQ"
     gene            6859..8817
                     /gene="acs"
                     /locus_tag="ACHJWB_003604"
     CDS             6859..8817
                     /gene="acs"
                     /locus_tag="ACHJWB_003604"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4315177.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            9017..9331
                     /gene="yjcH"
                     /locus_tag="ACHJWB_003605"
     CDS             9017..9331
                     /gene="yjcH"
                     /locus_tag="ACHJWB_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4315178.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     TFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9328..10977
                     /gene="actP"
                     /locus_tag="ACHJWB_003606"
     CDS             9328..10977
                     /gene="actP"
                     /locus_tag="ACHJWB_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4315179.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMTAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2572 a         3105 c         2923 g         2494 t
ORIGIN      
        1 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatacaa
       61 ctcaataaca taatcttcat caccagatga ttaatctgat ctccgctgca acaatttcgc
      121 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
      181 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
      241 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttatcga tcattgcgcg
      301 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
      361 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
      421 gtagtcgttt tgccacagat aatactcgcc cagtaacgcc cactgttcgc tgttttgtgg
      481 attagcgcgg attttatcct gtaatgcctg aagctgcgct tccggggttt gcttgctggc
      541 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
      601 gcttaacaga taactgccga cacagagaaa taccatcaat atcgtgagtg ttgtcagcat
      661 tttcaccggt attttgggct gtttcatcgc ttcgccctca ctcgccagag gatcagtgcc
      721 atcagcagta acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
      781 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
      841 ttcttcccct ccgccaccat gctgtaaacc tgatggcgca tactgactgc caccggcgcg
      901 ttggattcca gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
      961 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacgac ctgagcgcgc
     1021 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
     1081 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
     1141 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctccggttt tttcacccat
     1201 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
     1261 acgtgcttcg taaaaacgcc gctccggcat cagttcacca atgcgttgct gatggtcaaa
     1321 cagtgccact atcgcttttt cgctggtgta attgcctttg gcttgcagat cgaggcgctc
     1381 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
     1441 ctcctgacgg ctgacgctgg aaaccacgat ccccgcggca aataacagca cgcccgcatg
     1501 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
     1561 caccagcatc aacaggccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
     1621 gaggcgtccc cagcccagca gaccgtaaat catcggataa agcgttccca ccagcacgat
     1681 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
     1741 cgaaaaacgc accaccgcac caccgtcccg cgctcgccag ccatacagag ccagagacgc
     1801 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
     1861 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtacccagta gcgacagcat
     1921 cagagttact atcgccagca acagcgacca gtggcggaaa atctcccgct ggcgtgtcag
     1981 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
     2041 cggatcccag aaccaccagc cgccccagcc cagttcgcaa taggcccacc aggaaccgag
     2101 gatgatcccc gccgttaatg cgctccagcc aggtagtgcc cagcgccaac aaattctggc
     2161 gcaggcacca tcaaactcgc cgcgcagtaa actcgccagc gccacgctcg ccgccaccat
     2221 caaaccgcca tagccaaggt aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
     2281 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
     2341 ccacagcact acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
     2401 cggaaatagc ggatcggttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
     2461 aagcagcagc acccaaagca gcagcgaacc ttcatggccg ccccacaccg ccgccagttt
     2521 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
     2581 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
     2641 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
     2701 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
     2761 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtt aactgcccgg
     2821 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
     2881 gcacggtagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
     2941 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
     3001 ccaccagcgc acgcacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
     3061 gcaggaagat ttcaccccat accacgggga tttccatacg gtgtacaaac tgcgcttccg
     3121 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
     3181 acgagatgcc ggagaagagg aacagcaccg gcaggatcgg gttattgagg aacggatacg
     3241 atttcagcgc cgacagcaga aagccggtat aagcccccaa caacaccgcc aacaccagca
     3301 tcaatgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
     3361 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
     3421 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
     3481 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
     3541 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
     3601 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
     3661 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
     3721 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
     3781 tacgcggtac agcttcggtt tggttccctg cgccagcttg tagcggtaag taggcttctg
     3841 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
     3901 cgtcgggcaa gcctcaacgc acgcgggcag cttaccggcc tgcaaattgg tcttacggca
     3961 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
     4021 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
     4081 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggcg catgatcgca
     4141 gtgctggcaa gacttacgga agacgcgata tttcacatca ggaaattcgc cctgcggctc
     4201 gctacgaata atcatcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
     4261 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
     4321 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
     4381 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
     4441 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
     4501 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
     4561 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
     4621 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttgtacatc
     4681 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tggctggccg
     4741 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
     4801 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
     4861 tgctgggtaa cttcgtagcg atcaccagat gcctgcgccg caggggtagc ggtaattccg
     4921 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
     4981 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
     5041 acagaccgtt tttacgtgcc tgttcgtccc actgcgggat caccgttttg atgaagtcct
     5101 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
     5161 tcgagatatc cgggatctgg atttcatggg tgatgccttt ggtcgccagc aggcgcgcca
     5221 gtttggtgcg tgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
     5281 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
     5341 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
     5401 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
     5461 tcgactgctt acgttccgcg accacttttt gcaaggcagc tttgtcctgg gtatggcagt
     5521 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtatagagtt
     5581 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
     5641 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
     5701 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
     5761 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
     5821 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
     5881 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
     5941 gtttgccttt ggcgaactct ggtgaggcgg tattatggca gtcggcacaa cctaagttgt
     6001 tgacgatttc cgggccgccg cgcgcccact taccgtggaa gtagccatct tcgccgtctt
     6061 tctggatcag acgcgccaca tccgggcttt tacaactcca gcatgccatc ggtagcggac
     6121 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
     6181 aggcatgtcc acgcggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
     6241 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
     6301 aggagagata ttgatcggga tgctgcgggg caaaggtttc attcttcgct tccacagtta
     6361 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
     6421 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
     6481 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
     6541 actgtcatga cattgctcgc ccctatgtgt aacaaataac cacactgtga atgttgtctt
     6601 taatcaattg taagtgcatg taaaatacca ctttagagtt agtcagtatc ttcctctttt
     6661 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
     6721 caaaaattac cctgccgttt atttgcacaa ttctactttt gcgtgatctg tcgcccaaat
     6781 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
     6841 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
     6901 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
     6961 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
     7021 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
     7081 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
     7141 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
     7201 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
     7261 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
     7321 attggcgcgg tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
     7381 attattgatt ccaactcacg actggtgatc acttccgacg aaggtgtgcg tgccgggcgc
     7441 agtattccgc tgaagaaaaa cgttgatgac gcgctgaaaa acccgaacgt caccagcgta
     7501 gagcatgtgg tggtactgaa gcgtactggc gggaaaattg actggcagga agggcgcgac
     7561 ctgtggtggc acgacctggt tgagcaagcg agcgatcagc accaggcgga agagatgaac
     7621 gccgaagatc cgctgtttat tctctacacc tccggttcta ccggtaagcc aaaaggtgtg
     7681 ctgcatacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
     7741 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggtgac cggacacagt
     7801 tacttgctgt acggcccgct ggcctgcggt gcgaccacgc tgatgtttga aggcgtaccc
     7861 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
     7921 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gatcgagggt
     7981 accgaccgtt cgtcgctgcg cattctcggt tccgtgggcg agccaattaa cccggaagcg
     8041 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
     8101 cagaccgaaa ctggcggttt catgatcacg ccgctgcctg gcgctaccga gctgaaagcc
     8161 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
     8221 ccgctggaag gcgctaccga aggcagcctg gtgatcaccg actcctggcc gggtcaggcg
     8281 cgtacgctgt ttggcgatca cgaacgtttt gaacagacct acttctccac cttcaaaaat
     8341 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gataaccggg
     8401 cgtgtggacg atgtgctgaa cgtctccggt caccgtctgg ggacggcaga gattgagtcg
     8461 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
     8521 aaaggtcagg cgatctacgc ctacgtcacg cttaatcacg gggaggaacc gtcaccagaa
     8581 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
     8641 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
     8701 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
     8761 cctggcgtag tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
     8821 acaattgccg gatgcgagtc ggtatcggtt tgtagacctg ataagacgcg acagcgtcgc
     8881 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
     8941 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
     9001 ctctggagaa tctgtgatga atggcactat ttatcagcgg atagaagaca atgcgcattt
     9061 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
     9121 agtttatatc ggctttattt tactgatcac cttcgcgccc ggctggctgg gcacgccgct
     9181 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
     9241 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
     9301 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
     9361 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
     9421 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
     9481 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
     9541 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
     9601 atctccgcgc tggtgtttac ctccggctat gacggcttaa tttactcgct gggcttcctg
     9661 gtgggctggc cgatcatttt gttcctgatt gccgaacgtc tgcgtaacct ggggcgctac
     9721 acctttgccg atgtggcctc ttaccgtctg aaacaagggc cgattcgtat tctttcggcc
     9781 tgtggttctc tggtggtggt ggcgctttac cttatcgccc agatggtggg cgcaggtaaa
     9841 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
     9901 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cttgggtaca gattatcaaa
     9961 gccgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    10021 tttagcttca acaatctgtt cagtgaagcg atggcggtac acccgaaagg tgtcgacatc
    10081 atgaagccgg gcgggctggt gaaagatccg atctccgcgc tctctctggg tctgggactg
    10141 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    10201 cgcgaagcac gtaagagcgt gttctacgcc accgggttta tgggctactt ctatattctg
    10261 acctttatta tcggcttcgg cgcgatcatg ctggttggtg cgaatccgga atataaagac
    10321 gcggcgggcc atctgattgg tggtaacaac atgacggccg ttcacctggc gaatgcagtg
    10381 ggcggcaacc tgttcctcgg ttttatttca gcggttgctt ttgccaccat tcttgcggtg
    10441 gttgctggtc tgacgctggc gggcgcatca gcggtttcgc atgacctgta cgctaacgtc
    10501 ttcaaaaaag gcgcgaccga acgtgaagaa ctgcgggtat cgaaaatcac cgtgctgatt
    10561 ttaggtgtga ttgccattat cctcggcgtg ctgtttgaga atcagaacat cgccttcatg
    10621 gtaggcctgg cgtttgccat cgcagcgagc tgtaacttcc cgatcattct gctttctatg
    10681 tactggtcga aactgaccac tcgtggcgcg atgatgggtg gctggctggg gctgattacc
    10741 gcagtggtac tgatgatcct cggcccgacg atttgggtac agatccttgg tcacgaaaaa
    10801 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    10861 tggttcttct cggcaaccga taactcagcg gaaggtgcgc gcgagcgtga actgttccgt
    10921 gcacagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    10981 ctccccttcc ccggtcgcct gaccggggaa tactcttcct ctccagcatg catcaccttt
    11041 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtc
//
LOCUS       DAZFAH010000150        10887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFAH010000150 DAZFAH010000000
VERSION     DAZFAH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.151"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..707
                     /gene="dps"
                     /locus_tag="ACHJWB_003607"
                     /gene_synonym="pexB"
     CDS             204..707
                     /gene="dps"
                     /locus_tag="ACHJWB_003607"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4315180.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1111..1857
                     /gene="glnH"
                     /locus_tag="ACHJWB_003608"
     CDS             1111..1857
                     /gene="glnH"
                     /locus_tag="ACHJWB_003608"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4315181.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKSSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1996..2655
                     /gene="glnP"
                     /locus_tag="ACHJWB_003609"
     CDS             1996..2655
                     /gene="glnP"
                     /locus_tag="ACHJWB_003609"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4315182.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2652..3374
                     /gene="glnQ"
                     /locus_tag="ACHJWB_003610"
     CDS             2652..3374
                     /gene="glnQ"
                     /locus_tag="ACHJWB_003610"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4315183.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3491..5716
                     /gene="ybiO"
                     /locus_tag="ACHJWB_003611"
     CDS             3491..5716
                     /gene="ybiO"
                     /locus_tag="ACHJWB_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4315184.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGESLSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALTNVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASTYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(5713..6639)
                     /gene="rlmF"
                     /locus_tag="ACHJWB_003612"
     CDS             complement(5713..6639)
                     /gene="rlmF"
                     /locus_tag="ACHJWB_003612"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4315185.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6915..7175
                     /gene="mcbA"
                     /locus_tag="ACHJWB_003613"
     CDS             6915..7175
                     /gene="mcbA"
                     /locus_tag="ACHJWB_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4315186.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7440..9722
                     /locus_tag="ACHJWB_003614"
     CDS             7440..9722
                     /locus_tag="ACHJWB_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4315187.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHITQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9764..10441
                     /gene="ybiX"
                     /locus_tag="ACHJWB_003615"
     CDS             9764..10441
                     /gene="ybiX"
                     /locus_tag="ACHJWB_003615"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4315188.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10515..10781
                     /gene="ybiI"
                     /locus_tag="ACHJWB_003616"
     CDS             10515..10781
                     /gene="ybiI"
                     /locus_tag="ACHJWB_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4315189.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT         2667 a         2833 c         2750 g         2637 t
ORIGIN      
        1 ttattccgaa aattcctggc gagcagataa ataagaattg ttcttatcaa tatatctaac
       61 tcattgaatc tttattagtt ttgtttttca cgcttgttac cactattagt gtgataggaa
      121 cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat tactgggaca
      181 taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca aaagcgacca
      241 atctgcttta tacccgcaac gatgtctccg acagcgagaa aaaagcaaca gtagagttgc
      301 tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa gcgcactgga
      361 acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc cgcaccgcac
      421 tgatcgatca tctggatacc atggcagaac gtgcagtgca gctgggcggt gtagctctgg
      481 ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg gacatccaca
      541 acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct aatgacgtac
      601 gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc gccgcgtctc
      661 gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc catcgctgat
      721 ggtgcagaac tttagtaccc gataaaagcg gcttcctgac aggaggccgt tttgttttgc
      781 agcccacctc aacgcactta tttagtgcat ccatctgcta tctccagctg attaagtaaa
      841 ttttttgtat ccacatcatc acacaatcgt tacataaaga ttgttttttc atcaggtttt
      901 acgctaaata atcactgtgt tgagtgcaca attttagcgc accagattgg tgccccagaa
      961 tggtgcatct tcagggtatt gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa
     1021 aaatcaagga gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc acgcagggga
     1081 tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt ttcactggct
     1141 gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg
     1201 acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt gggctttgac
     1261 gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact gaagccgatg
     1321 gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc gctggcgggc
     1381 attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta ctacaaaagc
     1441 ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa agatctcgac
     1501 gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg attacgcgaa agcaaacatc
     1561 aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga actgggcacc
     1621 aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat caaaaccgcc
     1681 ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata cggtattgcg
     1741 ttcccgaaaa gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc
     1801 gagaacggaa cttacaacga aatctacaaa aaatggttcg gtactgaacc gaaataataa
     1861 cgctacacct gtaaagcgca ctggcagttc cctctcccct ctggggagag gattagggtg
     1921 aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt tacaccacgg
     1981 taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat tccgcttctg
     2041 attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg cggtctggta
     2101 atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg
     2161 ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg tccaggtgat gtttatctat
     2221 ttcgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc ggcggtggtc
     2281 accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct
     2341 atccacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg ggaaaccatt
     2401 cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg
     2461 atcatcagca ttaaagacac ctcgctgttt attgtgatcg gcgtggcgga actgacccgt
     2521 caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag cgccgtggcg
     2581 gtgttctatc tgattattac cctggtgctg agctttattc tgcgtcgtct ggaaagaagg
     2641 atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca acccaggtgc
     2701 tgcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc gggccgtccg
     2761 gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc acctccggcg
     2821 atctgattgt cgatggcctg aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc
     2881 aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg gcgctggaaa
     2941 acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg
     3001 cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac ccttccgaac
     3061 tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa
     3121 tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat gaagtgctga
     3181 aggttatgca ggatctggct gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg
     3241 gttttgccga gaaagtagct tcgcggctta tctttatcga caaaggccgg attgcggaag
     3301 atggcgatcc gcaggtattg atcaagaacc cgccgagcca gcgcttgcag gaatttttgc
     3361 agcacgtctc ttaacttctc tgcctccctt tcacccgaaa gggaggcaca ccagattcct
     3421 ctcatttaaa atcgcccctc ccccagcatc tatacttatc tttttgctct gttttctcac
     3481 tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctactgggcg cacctgccca
     3541 cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga ctgaaccggc
     3601 cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga
     3661 taacgacacc tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg cgacgccccc
     3721 tgcggaaccg gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca
     3781 aaaggtcacc gaagtcagcc gccattatgg tgaatccctt tccgcccgct tcgggcaact
     3841 ttatcgcaat atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc
     3901 gctgagccat ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct
     3961 gtgcgcactg ccgctgtatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg
     4021 cagcaactgg ttgcagcttc cggcgatgat tatcggggcg tttattatcg acctgctgtt
     4081 actggccctg acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg
     4141 caccatcgct ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa
     4201 agccgtacta cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca
     4261 ggacgagagc gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta
     4321 tggcctgatt gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct
     4381 gacgaacgtc atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa
     4441 taaaaaagag attacccagc atttgctcaa ctttgcggag cattcgctgg cctttttcag
     4501 cctgtttatc cgcgcctttg cgctggtgtg gcactggctg gcaagcacct attttatcgt
     4561 gctgtttttc ttttcgttgt tcgatccggg caacagcctg aaatttatga tgggtgcaac
     4621 ggtgcgcagc ctggcgatta ttggtattgc agcgtttgtt tccggtatgt tttcccgctg
     4681 gctggcaaaa accatcactc tctcgccaca tactcagcgt aactatccag agttgcaaaa
     4741 acggctgaat ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt
     4801 ggcggtaatg ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa
     4861 cggcgcgggg cagaaaaccg tagatatcct gatccgtatc gcacttattc tttttttctc
     4921 ggcggttggc tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca
     4981 tggccgcccg ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct
     5041 ggcggtgatt atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat
     5101 cgcgccattg ctggcagggg ccggggcatt aggtctggct atttcgtttg gttcgcaaac
     5161 gctggtgaaa gatatcatta ccggggtgtt tattcagttt gaaaacggca tgaacactgg
     5221 agatttggtg actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt
     5281 gggcgtgcgc caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt
     5341 tgctaacttc gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga
     5401 agatgctgat aaagccaatc aggcactgaa agatgcggta gcggaattga tggaaaacga
     5461 agaaattcgc gggctgatta ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa
     5521 taccgcgttt acactgcgtg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg
     5581 ctttgccctc gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt
     5641 gcagacttat caggtgctgc ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg
     5701 ggagccaacg ctttaacgtt ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa
     5761 ggtccaggca ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt
     5821 caccgcgccc acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac
     5881 cagcgaggta aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt
     5941 gataaagttg acttcaccgc cttcacacca caactcctgt tgctgaccac caaagttcag
     6001 tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgccgc
     6061 agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttgtg
     6121 gatgatgccg ttaaaaatcg ccccactctc tttttgccga cgcagacgaa tggcgcggtt
     6181 aagccccgga ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc
     6241 actaccggta aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc
     6301 accaacgccg atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag
     6361 taaatcggca aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc
     6421 gtcggggata tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc
     6481 cttcaccgcc agcggattgg caaagtctac gctttgctcc cctgcgggtg taagcgtgag
     6541 gaattgcctg agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg
     6601 acggttgcgc ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg
     6661 gcgtaagata cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa
     6721 aaccttaaca tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca
     6781 ttgatttgtc ttattttgta aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt
     6841 ttttgcgtca ttttcatcgc ccaggcgacc tacacttatc aggcactacc cgttgtattt
     6901 atgaggatgt cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa
     6961 tctctctcac ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc
     7021 ccgccggtac cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg
     7081 cagaaaaagc gcgcgagcaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg
     7141 accagatgtt cggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc
     7201 tctggatgca gttttcgcct gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa
     7261 ataaatgtat ttctttttcg cgcaatgggt gatagaaaat cgcgccaagt gataatactt
     7321 atcaaaatta ttatcacttg cacgaacact atcacgagat taacagtggc atcgcatccg
     7381 cagagaggct ttctcgtggc agtgaaaatt tcaacatata aaaaaaagtc acctgcaaaa
     7441 tggaaaacaa tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc
     7501 tttgtattgg catcacgcct gtggctcagg cactcgccgc cgaagggcaa actaacgcgg
     7561 atgacacgct ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg
     7621 atccgaaatt ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac
     7681 aagtgattaa agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg
     7741 tgggtgcgtt ttttgcgggt gagaacggta actccaccac tggcgacgcc atttatatgc
     7801 gcggtgctga tacctctaac agtatttata ttgatggcat tcgcgatatc ggcagcgtct
     7861 cgcgcgacac cttcaatact gagcaggtcg aagtgattaa agggccttcc ggcaccgact
     7921 acgggcgcag cgcgccgacg ggctcgatca atatgatcag caagcagccg cgcaatgatt
     7981 ccggcattga cgcctccgcc agtattggca gcgcctggtt ccgccgcggc acgctggacg
     8041 tcaatcaggt cattggtgat accactgcgg tgcgcctgaa tgtgatgggc gaaaaaacgc
     8101 acgatgccgg acgcgacaaa gtcaaaaatg agcgttacgg cgtcgcccct tctatcgctt
     8161 ttggccttgg tacagcgaat cgtttgtatc ttaattatct gcatatcacc cagcacaaca
     8221 cgccagacgg cggcattccg accatcggtt tgccgggtta ttctgcccca tctgcgggaa
     8281 cagcggctct gaatcattcc ggaaaagttg atactcataa cttttacggc acggattccg
     8341 attacgacga ttcgaccacc gacaccgcca ccatgcgttt tgagcacgac atcaacgata
     8401 acaccaccat tcgcaatact acccgttggt cgcgcgtaaa gcaggattac ctgatgacgg
     8461 cgattatggg cggggcgtcg aatattactc agcccaccag cgatgtgaat agctggacct
     8521 ggtcacgcac ggcgaatacc aaagatgtga gtaataaaat tctcaccaac cagaccaacc
     8581 tgacctcgac gttctatacc ggttctatcg gtcatgatgt cagtaccggc gtggaattta
     8641 cccgtgaaac gcagactaac tacggcgtta atccggtgac gttacccgcg gtaaatattt
     8701 atcatcctga cagcagcatt catcccggcg gcctgacgcg caacggcgca aacgccaatg
     8761 gtcagacgga taccttcgca atttacgcct tcgatacgct gcaaatcacc cgtgattttg
     8821 agctgaacgg cgggatccgt ctggataatt atcatactga atatgacagt gccaccgcct
     8881 gcggcggcag cggacgcggt gccatcacct gcccaactgg tgtggcaaaa ggttctccgg
     8941 tcaccaccgt cgacaccgcc aagtcgggca atctgatgaa ctggaaagcc ggggcgctgt
     9001 atcacctgac ggaaaacggc aatgtctata ttaactatgc cgtttcccag cagcctccgg
     9061 gcggcaacaa cttcgccctt gcgcagtctg gcagcggtaa cagtgccaac cgcaccgatt
     9121 ttaaaccgca aaaagccaac accagcgaga ttggcaccaa atggcaggtt ctggataaac
     9181 gtctgttgct caccgccgcg ctgttccgta ctgatatcga aaatgaagtt gagcaaaatg
     9241 atgacggaac ttactcgcaa tacggtaaga aacgcgtcga aggctatgag atatccgtgg
     9301 ccgggaatat cactcccgcg tggcaggtga ttggcggcta tacccagcaa aaagcaacca
     9361 tcaaaaacgg caaagatgtt gcccaggatg gttcctcatc gctgccgtat accccggagc
     9421 acgccttcac cttatggagc caatatcagg caaccgacga tatctctgtt ggcgcgggcg
     9481 cacgctatat cggcagtatg cataaaggtt cagacggcgc ggtgggaacg ccagcattta
     9541 ccgaaggtta ctgggtcgcc gatgccaaac tggggtatcg ggttaatcgc aatctcgact
     9601 tccagctaaa cgtctacaac ctgtttgata ccgattacgt cgcctcaatc aacaagagcg
     9661 gctaccgtta tcacccgggc gagccaagaa ccttcttgct cacagccaat atgcatttct
     9721 gattcagatg tggggcgcag gccccacttt ttggagaaat tgtatgatgt accacattcc
     9781 cggcgtgtta tcgccacagg acgtcgctcg ttttcgcgaa caactggaac aagccgaatg
     9841 ggtggatgga cgcgtcacca ccggcgcaca aggtgcgcaa gttaagaaca atcaacaggt
     9901 cgacacccgc agcacgttat acgccgccct gcaaaatgag gtgctgaacg cggttaacca
     9961 acatgcttta ttctttgccg cggccttgcc gcgtaccctt tccacgccgc tgtttaatcg
    10021 ctatcagaac aatgaaacct atggtttcca tgtggatggc gcagtacgca gccatccaca
    10081 aaacggctgg atgcgtactg acctttctgc cacgctgttt ttaagcgatc cacaaagcta
    10141 cgacggcggc gaactggtcg ttaatgacac cttcggacaa catcgggtaa aactcccggc
    10201 aggcgatctc gtgttgtatc cctccagcag cctgcattgc gtgacacccg taacccgcgg
    10261 cgtacgagtg gcatcattta tgtggatcca gtcgatgatc cgcgatgata aaaagcgcgc
    10321 catgctgttt gaactggaca acaatattca gtcgctgaaa agccgctacg gcgaaagtga
    10381 agagatcctg tcgctgctta atctttatca taatctgctg cgggaatggt cggagatctg
    10441 atgcttgaac tgcccgagtt cgtccctgga ttagctacac ttaactgtac aagtattgat
    10501 atggggaggt cgatatggca tccggttggg ctaacgatga cgccgtcaac gaacagatca
    10561 acagtacaat tgaagatgcg attgcccgcg ctcggggtga aattccgcgc ggcgaaagcc
    10621 tggatgaatg tgaagagtgc ggtgccccca tcccacaggc ccgtcgggaa gccattcctg
    10681 gcgtgcgctt atgtattcat tgtcagcagg agaaagattt acaaaaacca gcttatacag
    10741 gatataatcg cagaggttcg aaagacagcc agttacgtta actacaactg gcgggatgta
    10801 tagcgagtat aatttgtatt ttgcgtggca ttccgtgcct ttaacgctgt cgtgactgcc
    10861 acgcggtata aaacaagttc ataagta
//
LOCUS       DAZFAH010000151        10864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFAH010000151 DAZFAH010000000
VERSION     DAZFAH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.152"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..852
                     /gene="adiY"
                     /locus_tag="ACHJWB_003617"
     CDS             91..852
                     /gene="adiY"
                     /locus_tag="ACHJWB_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4315190.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            989..2326
                     /gene="adiC"
                     /locus_tag="ACHJWB_003618"
     CDS             989..2326
                     /gene="adiC"
                     /locus_tag="ACHJWB_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4315191.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2430..4073
                     /gene="eptA"
                     /locus_tag="ACHJWB_003619"
     CDS             2430..4073
                     /gene="eptA"
                     /locus_tag="ACHJWB_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4315192.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISIYLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDLIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYFDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4070..4738
                     /gene="basR"
                     /locus_tag="ACHJWB_003620"
     CDS             4070..4738
                     /gene="basR"
                     /locus_tag="ACHJWB_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4315193.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYILPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4739..5839
                     /gene="pmrB"
                     /locus_tag="ACHJWB_003621"
     CDS             4739..5839
                     /gene="pmrB"
                     /locus_tag="ACHJWB_003621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4315194.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5815..5904)
                     /gene="pmrR"
                     /locus_tag="ACHJWB_003622"
     CDS             complement(5815..5904)
                     /gene="pmrR"
                     /locus_tag="ACHJWB_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4315195.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6016..7518)
                     /gene="proP"
                     /locus_tag="ACHJWB_003623"
     CDS             complement(6016..7518)
                     /gene="proP"
                     /locus_tag="ACHJWB_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO4315196.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7782..8660)
                     /gene="rdcB"
                     /locus_tag="ACHJWB_003624"
     CDS             complement(7782..8660)
                     /gene="rdcB"
                     /locus_tag="ACHJWB_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4315197.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8657..>10864)
                     /gene="crfC"
                     /locus_tag="ACHJWB_003625"
     CDS             complement(8657..>10864)
                     /gene="crfC"
                     /locus_tag="ACHJWB_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO4315198.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVRDAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEPLSE
                     MRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2716 a         2843 c         2677 g         2628 t
ORIGIN      
        1 ttatattcat catacaatta tataaccatt tcccggtatc gctttgcttt agcgagaact
       61 ggtcttttat tcgcactcag gagtacatgt atgaggattt gcagcgacca accttgtatt
      121 gttttattga ctgaaaaaga tgtctggata agggtgaatg ggaaagaacc tattagcctt
      181 aaagctaacc atatggcgtt attaaattgt gaaaataata ttatcgacgt ctcctctctt
      241 aacaacactt tggttgctca tattagtcac gacatcatca aagattacct ccggtttctg
      301 aataaagatc tctcgcaaat accagtatgg caacgtagcg ctacgcccat actcaccctg
      361 ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac acagcatgat gcccgcagaa
      421 actgagtcag aaaaggaacg aacacgcgca ttattattca cggtgctatc ccgttttctc
      481 gacagtaaaa aattcctttc attaatgatg tatatgttac gtaattgtgt aagtgacagc
      541 gtttatcaaa ttattgaaag cgatattcac aaagactgga atcttagtat ggtagccagt
      601 tgtttatgtc ttagcccaag tctgttaaag aaaaagctga aaagcgaaaa caccagttat
      661 agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa atgaattaat gatggacggt
      721 aaaaatatct ctcaggtatc acagtcctgc ggctacaaca gtacgtcgta ctttatttct
      781 gtctttaaag acttctacgg tatgacgccg ctgcattatg ttagtcagca cagagaacgc
      841 actgtcgcct gatttttaac cttaacgaag agctatatta ataacggcat cagcgataac
      901 ccggtctata ataattcaac tatcgaatgc aggcgtatga tatgacgtaa tttattgtca
      961 cgaagctcgc cttcgcagga gtttaattat gtcttcggat gctgatgctc acaaagtggg
     1021 cttaatcccc gtcaccctga tggtgtcggg gaatattatg gggtcaggtg tttttctgtt
     1081 acctgcaaac ctggcctcta ctggcgggat tgccatttat ggatggttgg tgacgattat
     1141 cggtgcactg gggctctcga tggtatacgc caaaatgtcg ttcctcgacc caagtcctgg
     1201 tggttcttac gcttacgccc gccgctgctt tggcccgttt ctcggttatc aaaccaacgt
     1261 cctctactgg ctggcctgct ggatcggcaa tatcgccatg gtggtcattg gcgtaggata
     1321 tttaagttac ttcttcccga ttctgaaaga tccattggta ttaaccatca cctgcgtcgt
     1381 ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg aaaatgatca cccgtgtgca
     1441 ggcagttgcc accgtgctgg cgctgatccc catcgtcggg attgccgtat ttggctggtt
     1501 ctggttccgt ggtgaaacct atatggcggc atggaacgtc agcggcctgg gcaccttcgg
     1561 tgcaattcaa agtaccctta acgttacgct gtggtcgttc atcggtgtgg aaagtgcctc
     1621 cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc cctatcgcca ccattggtgg
     1681 ggtattgatt gccgccgttt gctatgtact ttctaccacc gcgattatgg ggatgatccc
     1741 taatgccgca ctgcgcgttt ctgcttcgcc attcggtgat gccgcacgga tggcgttggg
     1801 tgacaccgcc ggggccattg tttccttctg cgcagctgcg ggttgcttag gttcactggg
     1861 cggctggacg ttgctggcgg gtcaaacggc gaaagccgct gccgatgacg gactgttccc
     1921 accaattttt gcccgtgtaa ataaagcggg tacgccagtg gcggggctga ttatcgtcgg
     1981 tattttgatg accatcttcc agctcagcag catttcacca aacgcgacca aagagttcgg
     2041 tctggtttct tccgtctcgg tcatctttac actggtgcca tatctttaca cctgtgcggc
     2101 gttactgctg ctcggacacg gtcactttgg taaagcacgc ccggcatatc tggcagttac
     2161 taccattgcc ttcctctact gcatctgggc cgtggtgggg tccggagcga aagaggttat
     2221 gtggtcattt gtcaccctga tggtcatcac cgccatgtat gcgctgaatt acaaccggct
     2281 acataaaaac ccgtatccct tagatgcacc aataagcaaa gattaattcc ccttaatcca
     2341 gcaaacataa aagccaacct taagaactta aggttggctt aattttgctt tgcgagcata
     2401 tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg cctactaaaa agaccctctt
     2461 tgaatttact cgcctggcta ttgttggccg ctttttatat ctctatctac ctgaatattg
     2521 ccttttttaa acaggtgttg caggcgctgc cgctggattc gctgcataac gtactggttt
     2581 tcttgtcgat gccggtcgtc gctttcagcg tgattaatat tgtcctgaca ctaagctctt
     2641 tcttatggct taatcgacca ctggcctgcc tgtttattct ggttggcgcg gctgcacaat
     2701 atttcataat gacttacggc atcgtcatcg accgctcgat gattgccaat attattgata
     2761 ccactccggc agaaagttat gcgctgatga caccgcaaat gttattaacg ctgggattca
     2821 gcggcgtgct tgctgcgctg attgcctgct ggataaaaat caaacctgcc acctcgcgtc
     2881 tgcgcagtgt tcttttccgt ggagccaata ttctggtttc tgtactactg attttgctgg
     2941 tcgccgcact gttttataaa gactacgcct cgttgttccg caataacaaa gagctggtga
     3001 aatccttaag cccctctaac agcattgttg ccagctggtc atggtactcc catcagcgac
     3061 tagcaaatct accgctggtg cgaattggtg aagacgccca ccgcaacccg ttaatgcaga
     3121 acgaaaaacg taaaaatttg accatcctga ttgtcggcga aacctcgcgg gcggagaact
     3181 tctccctcaa cggctacccg cgtgaaacta acccgcggct ggcgaaagat aacgtggtct
     3241 atttccctaa taccgcatct tgcggcacgg caacggcagt ttcagtaccg tgcatgttct
     3301 cggatatgcc gcgtgagcat tacaaagaag agctggcaca gcaccaggaa ggcgtgctgg
     3361 atctcattca gcgagcgggc atcaacgtgc tgtggaatga caacgatggc ggctgtaaag
     3421 gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct gaatctgcct ggtcagtgca
     3481 tcaacggcga atgctatgac gaagtgctgt tccacgggct ggaagagtac atcaataacc
     3541 tgcagggtga tggcgtgatt gtcttacaca ccatcggcag ccacggtccg acctattaca
     3601 accgctatcc gccgcagttc aggaaattta ccccaacctg cgacaccaat gagatccaga
     3661 cctgtagcaa agagcaactg gtgaacactt acgacaacac gctggtttac ttcgactata
     3721 ttgttgataa agcgattaat ctgctgaaag aacatcagga taaatttacc accagcctgg
     3781 tttatctttc tgaccacggt gaatcgttag gtgaaaatgg catctatctg cacggtctgc
     3841 cttatgccat cgccccggat agccaaaaac aggtgccgat gctgctgtgg ctgtcggagg
     3901 attatcaaaa acggtatcag gttgaccaga actgcctgca aaaacaggcg caaacgcaac
     3961 actattcaca agacaattta ttctccacgc tattgggatt aactggcgtt gagacgaagt
     4021 attaccaggc tgcggatgat attctgcaaa cttgcaggag agtgagtgaa tgaaaattct
     4081 gattgttgaa gacgatacgc tgttattgca gggattgatt ctggcggcgc aaaccgaagg
     4141 ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa caaagccttg aggccggtca
     4201 ttacagcctg gtggtactgg atttagggtt acccgacgaa gatggactgc attttctcgc
     4261 ccgtatccgg cagaaaaaat acatcctgcc agtactgatc ctcaccgctc gcgatacgct
     4321 gaccgacaaa atcgctgggc tggatgtcgg tgccgatgac tatctggtga agccttttgc
     4381 gctggaagag ttacacgccc gtatccgcgc cctgctacga cgccataata atcagggcga
     4441 aagtgagctg attgttggca atctgacgct gaatatgggt cgccgtcagg tatggatggg
     4501 cggtgaagag ttgattctga cgcccaaaga atatgctctg ctgtcacggt taatgctcaa
     4561 agcaggcagt ccggtgcatc gggaaattct ctacaacgac atctataact gggacaatga
     4621 accctcgacc aacaccctgg aagtgcatat ccacaatctg cgcgacaaag tgggcaaagc
     4681 ccgtatccgc accgtgcgcg gctttggcta tatgctggtc gcgaatgagg aaaactaatt
     4741 gaatctgatg cattttctgc gccgaccaat atcgctgcgc caacggctga tattgaccat
     4801 cggggccatt ttgttggtgt ttgagctgat cagcgtcttc tggctatggc atgaaagtac
     4861 cgagcagatt cagctgtttg agcaggcgct acgcgacaat cgcaacaacg atcgccacat
     4921 catgcgtgag atccgcgagg cggtcgccag cctgattgtc cccggcgtgt ttatggtcag
     4981 cctgacgcta tttatctgct atcaggcggt acgccgcatc acccgcccgc tggcggagct
     5041 gcaaaaagag ctggaagcgc gcaccgccga caacttaacg cccattgcca ttcacagcgc
     5101 caccctcgaa atcgaagcgg tggtttcggc gttgaacgat ctggtcagtc gcctgaccag
     5161 cacgctggat aacgaaaggt tgtttaccgc tgacgtcgcg cacgaactgc gaacgccact
     5221 ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg catcacattg atgtagcacc
     5281 gttagtggca cggcttgatc agatgatgga gagcgtctcc cagctgctgc aactggcgcg
     5341 tgccggacag tcattttctt ccggtaatta tcaacatgta aaactgctgg aagatgtcat
     5401 tctcccctcg tatgacgaac tcagtaccat gctcgaccag cgacagcaaa acctgctatt
     5461 gccagagagc gccgccgaca tcaccgttca gggtgatgca accctgctgc ggatgttatt
     5521 gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa ggcagcaaca ttatgattaa
     5581 gctgcaagaa gatggcggag cggtcatggc agttgaagat gaaggaccag gtattgatga
     5641 gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg gacagccgtt atggcgggat
     5701 tggtctgggg ttaagtattg tcagccgcat tacacagttg catcacgggc agtttttcct
     5761 gcaaaaccgg caagagactt ccggcacgcg ggcctgggta cggctgaaga aagatcagaa
     5821 cgtggcaaac cagatataaa gaaagctgct gaccaccagc acgctgaaca cggtagttaa
     5881 actttcataa acacggtttt tcattgcact ctcctccgtt aacctggagg agagtatgcg
     5941 cgtcagagat taagtgaacc ttaagagttc aattggcctg ataagacagc gtcacatcag
     6001 gccatccgtt tcagcttatt catcaattcg cggatgttgc tgcaccaggc gggtacgttt
     6061 cgcctgcaaa tcggcaatct cgtggtcaat attatcgatt ttctgctcga tattatcgta
     6121 atgctcgacg agaatttcct tcgcttcctg tatatctgac gccgccggtg tcgcaccttt
     6181 caacggacga tttgccgtct ctttcatggt tacgccggtg attaaaccaa tcaccgccac
     6241 taccatcagg taataggcag gcatcatcag attctgcgag ctttcgacca gccaggccgc
     6301 cagtgttggc gtcagaccgg caaccagcac cgaaatatta aatgccgccg ccagcgcgct
     6361 gtaacggata tgcgtcggga acatcgctgg caaggtagaa gccataacgc ccgtaaagca
     6421 gttaaggatc accgccagca tcagtaaccc ggcaaaaatc aggccgatga cgttactgtt
     6481 aatcagaata aacgccggga tcgccaacac aaacagtgca acactaccaa gtagcacaaa
     6541 cggacgacgg ccaaaacggt cactcagcaa gcccatcacc ggctggacaa acagcatacc
     6601 gatcataatg gcgataataa tcagcacccc gtggtcttcg gagtaatgca agttatgcga
     6661 caaataactc ggcatatagg tcagcaacat atagtaagtc acgttggtgg caattaccag
     6721 accaatacat gtcaacaggc tgcgccagtg tttggtggca atctctttaa acgagacttt
     6781 cgggccatcc tgtaaacctt cgcggtcgcc ctgttccagt ttatcaacat gctgctggaa
     6841 cgctggggtc tcttccaggg catggcgcag gtaaagcccg ataatcccta acggcagagc
     6901 aataaagaac ggaatacgcc agccccagtc gaggaagttc gcttcgccga caatggtcga
     6961 aattaacacc accacgcccg cacccagcac aaacccggca atagaaccga agtccagcca
     7021 gctgcccata aagccacgtt tacggtcagg tgagtattcc gcaacaaata tcgacgcccc
     7081 ggtatattca ccgccgaccg agaaaccttg tgccatctta cagatcaaca gcagaatcgg
     7141 tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca atacagaacg tactgatcga
     7201 cataatcaca atagtgatag cgaggatctt ctggcgacca tatttatcgc ccaacatacc
     7261 aaagaagagt ccgccaagcg gtcgaatcag aaagggaacg gagaaagtgg caagtgcagc
     7321 aaccatctgc acgctggggt cagcccccgg gaaaaaaact ttacctaatg cgtaagcaac
     7381 aaaaccataa acaccaaaat cgaaccattc cattgcatta cccagtgatg ctgcggtaat
     7441 ggctttacgc agtttaccgt catcaataat ggtgacatca cgaagggtaa ttggttttac
     7501 ttttttcctt ttcagcatag ctttcctcgc agagttggcc catttccgca aacgccagaa
     7561 aaatcagcgc gacggccgca aaaggggagc gggaattgca ggatgcaatc tctgtaacaa
     7621 gcgtagcggg tttgcttaca ccctccggtt aaggaatgta caatcaaacc agttgacgcc
     7681 tgaattgggc agttaatgac ctttttaccc taccagtgtt taaatttgtg atcaacttca
     7741 cacatggatt tcgtttagga ctcattgatg taactgatta tttacgccaa atttacagag
     7801 aagatttcct cgagcattgc ccccgccaga cgctgcgctg aacagagtcg tggcatcccc
     7861 agcgcgacag tttgccaacg ttgggtcact gaccagcaaa cgggttgttt atctgccgtg
     7921 gtccactttc ccagccaact taaaaaatca gcatgatccc cgcccgtcgg aatggtcgaa
     7981 gaggcaaacc actgatggta atagtgacgc gtggtcggtg ccgcattgat actctctgcc
     8041 agatgttgtg tcgctttcac gcgcaaatca cccagcaaca acgccaccgc ttgcgggctt
     8101 cccagacggc aggcatcgcc aaatgccccc caacgcaact cttcgagaac gataaaacag
     8161 cgcccaggca acgaccaggc gttatacgcc ccagcacgcc agcgggtaaa aatttgctcg
     8221 ctctgatcac ctgcctgcac cagtaggcct cgttgcgtta acgccttttc cttccagcgg
     8281 caacgggctg aaaaccactc atacaaggcg gcaatttgct gggggagctc cgggaaagca
     8341 gagtgctggc ggtgatccat tttttgcagc agcgccagat gctgcgcgat ctgcgtcagc
     8401 gccagatgac cgggatccag catcgacgcc agtttttcca ccattgtcag tcgcaggcta
     8461 agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa gctgcacgcg cgcaagaagt
     8521 tcctgcatca gtcgttcacg aaactgctgc tgatgggctt gtggaaggcg ggcatcatcg
     8581 ccctgcgcca gatccactaa aaagcgggga taaaccgact ccagcacgcg accggggccg
     8641 tccagtaacg tcttggtcaa tatcgttctg ccgtgaaaag tgtttgaata tcatcgcgta
     8701 acagctgggc gtcggtgtaa atccagccgt gagtcatcac agtctgctgc aattgctgct
     8761 gcatcagcct gaccaccgat tcattttgtt gacgcagagc caggctttcg cgtaaacgcg
     8821 tctgtaattc cgtcaaacat gaagcgaact cagcgaaaaa agtattcatg cctgccgtaa
     8881 cagattcatc gacctgctct gccagaactt tagccatttg ttggcaataa agatcgactt
     8941 ctgcgcttaa tgctcgttgc aacacactgt aatcaaccgt ttctgtcggg gatttctcat
     9001 ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa agtctcacgc acaacgccta
     9061 aacgcgtgct ctgctcgtcc gttgcatcct ggcgcgaaat gacttcactg aacagctggc
     9121 gagtgttgac gtggggaact actccgtgaa aaacaggaaa atgaaaccca ggacgaaacc
     9181 ctgactcgct caattccatt ttgacttgtt gctcaatggg gcgaataaca tcggttaaca
     9241 cccggcaaag ggtggattcc agctcggcaa aacgcagcgt aaagtcgcga ctgatggtgt
     9301 tctgcgccgt ctgtaacagt gtctcacagc gggtacgcat ctcgcttaac ggctccgaat
     9361 catcctgaaa caaggcggct aactgcgcat tcagcgcatc ttgttgttga cgcagaaagt
     9421 ggttggcgga ggtcagggcc agctcgattt catgtttaat ctcgccgctc acctgcgcct
     9481 gattgagttg caatagctgc aaactttctt cgacctgatg gatattttgc cgcaattgtt
     9541 cacaagcgac gtttaacccg tgcgcacgaa aatccaggta ttcccgcgcc tgctgcgcgt
     9601 aattcaacag tttatgcgca gcagatcgca aagcatacaa cgaggcgtta gcgtaagcgg
     9661 catgaagcaa cgcctgaatt ggctgggcga acagcgaatc ttcccacaac tgatcggcag
     9721 catgacgaat atgttcgagg tccgccagat cggcatgacg ccagcgcctg ccgagcgcgg
     9781 catgggcaaa atcttccacc cagcgttgtt gctctggcgc tggtaactta ccgttgttgg
     9841 ctaactcatg gcgcgcccga ttcgccaggt agccccacat cgacgacacc ggaaatatct
     9901 gctgtggcgt aatacagcct ttcatcagcg tcccggaaat cagtgcccgc acctggtcgg
     9961 cgtcgtcact gttacgatcc tgttgatcga acttattgac cagcacatac agcggcaccg
    10021 attgccccac cgccaaaatc gcatcacgga cctcttcatc ggagatcgat ttcagttgcg
    10081 tataatccag caccgccagt accgccgagg cgcgtgccag ctgctggtta agcatttttt
    10141 gcagatgcgg ttgcccggct tcatttggcc cgggggtatc cagtaacgtc aactgaccgg
    10201 gataactctc cagccccgcc agatggacaa actccacttc aatcacggga atatgctcaa
    10261 tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc cttcgccagt cgcactaaat
    10321 cattcaaact tttcagacaa tgaaaaatag gctgggcacc cagataatat ttttcgaaag
    10381 cgacgccatt ttcgatccgc tgcataagcg cacgcatatc tttatctatt tccagcacat
    10441 cggtcagatg cttaatatcg caatcacgca ggcgctgttg taattgttga attaaacaat
    10501 cgattggcgc gacatgtgaa aaatgcagta ccggttcctt ttgcccgggc gtatggcgaa
    10561 taagcgtcgg cagcgcagtc attgggcgat tacgattagg cagaacctcc gtaccaacaa
    10621 tggcattaat ggtggttgat ttccctgctt tcatggtacc gacaattgcc agcaccattt
    10681 ccagtcggga aattttacgc aactcattat tcagcatcgc gtgacgttcg gcgatattag
    10741 gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc gggtacagag agaggcattt
    10801 tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag gcgttcagcc tcctgactta
    10861 actc
//
LOCUS       DAZFAH010000152        10829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFAH010000152 DAZFAH010000000
VERSION     DAZFAH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.153"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(400..1593)
                     /locus_tag="ACHJWB_003626"
     CDS             complement(400..1593)
                     /locus_tag="ACHJWB_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4315199.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1729..3453
                     /gene="iucA"
                     /locus_tag="ACHJWB_003627"
     CDS             1729..3453
                     /gene="iucA"
                     /locus_tag="ACHJWB_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO4315200.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3454..4401
                     /gene="iucB"
                     /locus_tag="ACHJWB_003628"
     CDS             3454..4401
                     /gene="iucB"
                     /locus_tag="ACHJWB_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO4315201.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4401..6143
                     /gene="iucC"
                     /locus_tag="ACHJWB_003629"
     CDS             4401..6143
                     /gene="iucC"
                     /locus_tag="ACHJWB_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO4315202.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6140..7477
                     /gene="iucD"
                     /locus_tag="ACHJWB_003630"
     CDS             6140..7477
                     /gene="iucD"
                     /locus_tag="ACHJWB_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO4315203.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7483..9678
                     /gene="iutA"
                     /locus_tag="ACHJWB_003631"
     CDS             7483..9678
                     /gene="iutA"
                     /locus_tag="ACHJWB_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO4315204.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9936..10151)
                     /locus_tag="ACHJWB_003632"
                     /pseudo
     CDS             complement(9936..10151)
                     /locus_tag="ACHJWB_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            10238..10663
                     /locus_tag="ACHJWB_003633"
     CDS             10238..10663
                     /locus_tag="ACHJWB_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4315205.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            10660..10734
                     /locus_tag="ACHJWB_003634"
                     /pseudo
     CDS             10660..10734
                     /locus_tag="ACHJWB_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         2581 a         2817 c         2851 g         2580 t
ORIGIN      
        1 tccagctctt tcaggtgtca acgccactct gagagagttt tatgcatccc gtattgggca
       61 ctatgatgcc ggaaatctgc gaggctcgac acctctctga ttttcccctt tatttatcac
      121 ggtgccccag atctaatgtt ggggatgcgt tcatctcatt cccaatgagt aatcgcactg
      181 ttctcattgt ctgcaggcat ccggacagag ccatgtgatg ttgcactcac aaccagcatg
      241 acttcaatgc aacttaagtg acaccggccg gttttatacc gaacataccc tgtaccatgc
      301 ggtgcatttt tgccagtgtt actgcctgac atgctttcat cagacgcagg ttctctctca
      361 tatcctctga gttactccgg taaggtgatg atcatatctt tatttgatag cacggtaatg
      421 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgccg ccataatcag
      481 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      541 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      601 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      661 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      721 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      781 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
      841 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
      901 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
      961 gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1021 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1081 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1141 cagagcggcc atgcgattcc cttctcctgc tctctgcaat gcccagacct gacaggctgg
     1201 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1261 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1321 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1381 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1441 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1501 accgttctgc ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1561 tttttggggc gtatcttcga tcccaggtga cattttaatg tttcaactcc atgtattaat
     1621 tgtgtttatt tgtaaaatta atttatctga caataacatt tcttattgat aatgagaatc
     1681 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1741 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1801 actacagact ggaaactgac agaatacccg ccagacgaat tgatcatccc gctggatgag
     1861 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     1921 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     1981 cggcttatta ttgataagct acggcatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2041 ttccaccagc gcgtgctgga aagccacgcc catacgcaac aggcaattga tgcccgtcat
     2101 gactggaccg ccctgcgtga aaaagcgttg aattttggcg aagctgagca ggcgctgctg
     2161 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2221 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2281 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2341 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2401 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaag
     2461 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2521 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2581 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2641 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2701 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2761 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agaccaacgt actggtctcc
     2821 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     2881 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     2941 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3001 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3061 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3121 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3181 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3241 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3301 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3361 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3421 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3481 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3541 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3601 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3661 tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3721 accgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3781 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccgcagggt
     3841 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     3901 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     3961 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4021 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta ttttgaaatc
     4081 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4141 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4201 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4261 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4321 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4381 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4441 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4501 atgaccacta ctgcattaac ctgccgggag cacaatggcg ctttatcgct gaacgtggta
     4561 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4621 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4681 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4741 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4801 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     4861 aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg gtaaaacgtg
     4921 aacatatgat ctggcgctgc gacaacgatc tggatattca gcagttgttg actgccgcaa
     4981 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5041 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc accgacttca
     5101 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5161 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5221 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tatatcgctg
     5281 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5341 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5401 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5461 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5521 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5581 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5641 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5701 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5761 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     5821 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     5881 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     5941 tgagtgatta catgaacaaa catccacaaa tgtcagagcg ttttgcgctt ttctcactct
     6001 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggaccagg
     6061 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6121 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6181 atttaatctc agtattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6241 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6301 gactgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6361 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6421 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6481 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6541 gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6601 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6661 tcgtcggccg gacctgagtg gaaaacggat aactgtggtt ggtggaggac agagtggtgc
     6721 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6781 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     6841 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     6901 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     6961 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7021 ctcggtaaca actctggaaa gtagtggtcc gggctggaag ttattgatgg aacatcatct
     7081 ggatcggggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7141 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7201 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7261 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7321 ctggagatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7381 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccgcagc cggaggctgc
     7441 tgctttaact cactatacaa caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7501 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc ccagcaaaac
     7561 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7621 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7681 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagccgaacc
     7741 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7801 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     7861 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     7921 aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc tggcacaaaa
     7981 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8041 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8101 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8161 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8221 acgcagtact ataaaagtca gggggacgac aattacgggc ttaatctcgg gaaaggcttt
     8281 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8341 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgagacag
     8401 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggttctaccc gttcccgacg
     8461 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8521 ggcatgaaac tgactctgaa cagccaactt atggacggct ggcaaatcac ctgggggctg
     8581 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8641 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8701 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtc
     8761 agcggtggcg tacgctatca gtatactgag aacagggtag atgattttat cgactacacg
     8821 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     8881 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     8941 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9001 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9061 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttacc
     9121 ggtgacaacc tgcggactca aatcgcagca tattactcgc tttccaataa gagcgtggaa
     9181 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9241 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9301 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9361 ccatcgaaag cgacagctta cattagctgg gcgccggaac cgtggagtct gcgtgtgcag
     9421 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9481 gtcgatttta tcagtagttg gctgcttccg gtgggaacac tcagcttcag cgttgagaac
     9541 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9601 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9661 tactcagtgc tgttctgacg gggattcttt tgcaacaagg gtatgctgta aacaacatgg
     9721 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9781 ctccggaatg gactgttcac cactgaataa caatatgtct gttttatgga tctttgactg
     9841 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac aagtgaaccg
     9901 ccccgggttt cctggagagt gttttatctg tgaactcagg ctgccagatc atcgtttccg
     9961 atggaagcat aataagcttt ttctgcttct gccggaggag tatggcccag cctttccagc
    10021 aatcgtcgat tgttatacca gtccacccac gtgagtgtgg ccagttccac ttctgcacgg
    10081 tttttccagc tcttacggtg tattacctcc gctttgtaaa gaccattgat gctctccgcc
    10141 atcgcgttgt cagtaagcgt aaactgaccg ccgtatgtag ccatcagacg aaaattggta
    10201 acttagacgc ccatctgata tagacggaca tctaagtatg gaattacagg actggcgaaa
    10261 agaacctcgt aaaaactatt cgaatgaatt caaacttcgt atggtggaac tggcatcaca
    10321 accaggagcc tctgttgccc gtattgcccg ggaacacgat atcaatgata acctcctgtt
    10381 caaatggctc aggctctggc agaacgaagg gcgtatatca cggcgtcttc aggtaacgac
    10441 ctcttctgac actggcgttg aattattacc tgtagaaata acgccggatg agcagaaaga
    10501 accggtggcg gccattgcgc cgtctttatc cacttccact cagaccagtg tcagtgccgg
    10561 ctcctgcaag gtggagttcc gtcacggtaa catgacgctg gaaaatccat cgccagagct
    10621 gctcacgctg ttgatccgtg aactgaccgg gaggggacga tgatctcact cccgtcaggt
    10681 acccgcatct ggctcgttgc cgacgttacc gatatgcgta aatccttcaa cggatacgac
    10741 atatgctgaa ggaggagcag taatggatta agtgataaca aatacccaga aatagaggtt
    10801 caaatccagc ttagttagta cataaatac
//
LOCUS       DAZFAH010000153        10808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFAH010000153 DAZFAH010000000
VERSION     DAZFAH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.154"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            466..2394
                     /gene="thrS"
                     /locus_tag="ACHJWB_003635"
     CDS             466..2394
                     /gene="thrS"
                     /locus_tag="ACHJWB_003635"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4315206.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2398..2940
                     /gene="infC"
                     /locus_tag="ACHJWB_003636"
     CDS             2398..2940
                     /gene="infC"
                     /locus_tag="ACHJWB_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4315207.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3037..3234
                     /gene="rpmI"
                     /locus_tag="ACHJWB_003637"
     CDS             3037..3234
                     /gene="rpmI"
                     /locus_tag="ACHJWB_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4315208.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3287..3643
                     /gene="rplT"
                     /locus_tag="ACHJWB_003638"
     CDS             3287..3643
                     /gene="rplT"
                     /locus_tag="ACHJWB_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4315209.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3760..3884
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3766..3810
                     /gene="pheM"
                     /locus_tag="ACHJWB_003639"
     CDS             3766..3810
                     /gene="pheM"
                     /locus_tag="ACHJWB_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4315210.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4093..5076
                     /gene="pheS"
                     /locus_tag="ACHJWB_003640"
     CDS             4093..5076
                     /gene="pheS"
                     /locus_tag="ACHJWB_003640"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4315211.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5091..7478
                     /gene="pheT"
                     /locus_tag="ACHJWB_003641"
     CDS             5091..7478
                     /gene="pheT"
                     /locus_tag="ACHJWB_003641"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4315212.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7483..7782
                     /gene="ihfA"
                     /locus_tag="ACHJWB_003642"
     CDS             7483..7782
                     /gene="ihfA"
                     /locus_tag="ACHJWB_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4315213.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7883..8863
                     /gene="btuC"
                     /locus_tag="ACHJWB_003643"
     CDS             7883..8863
                     /gene="btuC"
                     /locus_tag="ACHJWB_003643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4315214.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            8926..9479
                     /gene="btuE"
                     /locus_tag="ACHJWB_003644"
                     /pseudo
     CDS             8926..9479
                     /gene="btuE"
                     /locus_tag="ACHJWB_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
     gene            9479..10228
                     /gene="btuD"
                     /locus_tag="ACHJWB_003645"
     CDS             9479..10228
                     /gene="btuD"
                     /locus_tag="ACHJWB_003645"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4315215.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10306..10770
                     /gene="nlpC"
                     /locus_tag="ACHJWB_003646"
     CDS             10306..10770
                     /gene="nlpC"
                     /locus_tag="ACHJWB_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4315216.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2706 a         2559 c         2932 g         2611 t
ORIGIN      
        1 cagaacgaaa tgtgcacgaa aaattgttat aagaattgtg tcacttttat cattgtgata
       61 tgaccacttc gtcattaatc tcatgagcgt ttaaaatact accgccaaat cacgcaacga
      121 actttgctgg attcagttta tgctgctgta aatccgctcg cagtaaacct ttcagacgca
      181 ccgtgatgtt atcagtggtt ctttaagcgt tttgcttgtg tactcactac aaacgaattg
      241 cgaatcgaat caatgtgaaa cggaaaggta caatctccct ttttttagtg ttaacatcgc
      301 tcaaccgggt ggaaggtgag aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa
      361 tttagcattt gttggctaga tggtttcgca atgaactgtt aataaacaaa tttttctttg
      421 tatgtgatct ttcgtgtggg tcaccactgc aaataaggat ataaaatgcc tgttataact
      481 cttcctgatg gcagccaacg ccattacgac cacgctgtaa gccccatgga tgttgcgctg
      541 gacattggtc caggtctggc gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt
      601 gatgcttgcg atctgattga aaacgacgca caactgtcca tcattaccgc caaagacgaa
      661 gacggtctgg agatcattcg tcactcctgt gcgcacctgt tagggcacgc gattaaacaa
      721 ctttggccgc ataccaaaat ggcaatcggc ccggttattg acaacggttt ttattacgac
      781 gttgatcttg accgcacgtt aacccaggaa gatgtcgaag cactcgagaa gcggatgcat
      841 gagcttgctg agaaaaacta cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt
      901 gaaactttcg ccaaccgtgg ggagagctac aaagtctcca ttcttgacga aaacatcgcc
      961 catgatgaca agccaggtct gtacttccat gaagaatatg tcgatatgtg ccgcggtccg
     1021 cacgtaccga acatgcgttt ctgccatcat ttcaaactaa tgaaaacggc aggggcttac
     1081 tggcgtggcg acagcaacaa caaaatgttg caacgtattt acggtacggc gtgggcagac
     1141 aaaaaagcgc ttaacgctta cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt
     1201 aaaatcggta aacagctcga cctgtaccat atgcaggaag aagcgccggg tatggtattc
     1261 tggcacaacg acggctggac catcttccgt gaactggaag tgtttgttcg ttctaaactg
     1321 aaagagtacc agtatcagga agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa
     1381 aaaaccggtc actgggacaa ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt
     1441 gaatactgca ttaagccgat gaactgcccg ggtcacgtac aaattttcaa ccaggggctg
     1501 aagtcttatc gcgatctgcc gctgcgtatg gccgagtttg gtagctgcca ccgtaatgag
     1561 ccgtcaggtt cgctgcatgg cctgatgcgc gtgcgtggat ttacccagga tgacgcgcat
     1621 atcttctgta ctgaagaaca aattcgcgat gaagttaacg gatgtatccg tttagtctat
     1681 gatatgtaca gcacttttgg cttcgagaag atcgtcgtca aactctccac tcgtcctgaa
     1741 aaacgtattg gcagtgatga aatgtgggat cgtgctgagg cggacctggc ggttgcgctg
     1801 gaagaaaaca acatcccgtt tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa
     1861 attgaattta ccctgtatga ctgcctcgat cgtgcatggc agtgcggtac agtacagctg
     1921 gacttctctt tgccttctcg tctgagcgct tcttatgtag gcgaagacaa tgaacgtaaa
     1981 gtaccggtaa tgattcaccg cgcaattctg gggtcgatgg aacgtttcat cgggatcctg
     2041 accgaagaat tcgctggttt cttcccgacc tggctcgcgc cggttcaggt tgttatcatg
     2101 aatattaccg attcgcagtc tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg
     2161 ggcattcgtg ttaaagcaga cttgagaaat gagaagattg gctttaaaat ccgcgagcac
     2221 actttgcgtc gcgtcccata tatgctggtc tgtggtgata aagaggtgga atcaggcaaa
     2281 gttgccgttc gcacccgccg tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc
     2341 gagaagctgc aacaagagat tcgcagccgc agtcttaaac aattggagga ataaggtatt
     2401 aaaggcggaa aacgagttca aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc
     2461 caggaagttc gcttaacagg tctggaaggc gagcagcttg gtattgtgag tctgagagaa
     2521 gctctggaga aagcagaaga agccggagta gacttagtcg agatcagccc taacgccgag
     2581 ccgccggttt gtcgtataat ggattacggc aaattcctct atgaaaagag caagtcttct
     2641 aaggaacaga agaaaaagca aaaagttatc caggttaagg aaattaaatt ccgtcctggt
     2701 acagatgaag gcgactatca ggtaaaactc cgcagcctga ttcgctttct cgaagagggt
     2761 gataaagcca aaatcacgct gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt
     2821 atggaagtgc ttaatcgcgt gaaagacgat ttgcaagaac tggcagtggt cgaatccttc
     2881 ccaacgaaga tcgaaggccg ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa
     2941 ggccttcaag tagcactgtc tgtggagcct ttgggcttca caggcattgt tcgcctttgt
     3001 ttcgtttatt aacaatgcga agtggaagtt attaaaatgc caaaaattaa gaccgtacgc
     3061 ggtgctgcta agcgcttcaa aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac
     3121 ctgcgtcaca ttctgaccaa aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc
     3181 atggtttcca aaggcgatct gggcttggta atcgcgtgcc tgccgtacgc ataagccgtt
     3241 aacgttttta actttttaat tagaatatag atacaggaga gcacatatgg ctcgcgtaaa
     3301 acgtggtgtt attgcacgcg cacgtcacaa gaaaattttg aaacaagcta aaggctacta
     3361 cggtgcgcgt tctcgcgtat accgcgttgc cttccaggct gttatcaaag ctggtcagta
     3421 tgcttaccgt gaccgtcgtc aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat
     3481 caacgcagca gcacgtcaga acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa
     3541 agcctctgtt gaaatcgacc gtaagatctt ggctgatatc gcagtattcg acaaagtagc
     3601 gttcaccgct ctggttgaaa aagcgaaagc agctctggca taagccagtt gaaagaggga
     3661 gctagtctcc ctcttttcgt ttcaacgcca tcaaaacatt gacttttatc gccgtagcct
     3721 tttcaataaa ggtcttttga agagtaccaa aaggtaacgc aagcaatgaa tgctgctatt
     3781 ttccgcttct ttttttactt tagcacctga atccaggagg ctagcgcgtg agaagagaaa
     3841 cggaaaacag cgcctgaaag cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa
     3901 ataaaaagcc agtctatcag gcgtaattgt cgcagccagt ctggacacgg ccagcgcgga
     3961 gaaaccggag cgtatttaag tacgtgagaa tttcgagcac tgcccgggac caaaatggca
     4021 agtaaaatag cctgatggga taggctctaa gtccaacgaa ccagtgtcac cactgacaca
     4081 atgaggaaaa ccatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc
     4141 caggcgtcag atgttgccgc gttagataat gtgcgcgtcg aatatttggg taaaaaaggg
     4201 cacttaaccc ttcagatgac gaccctgcgt gagctgccgc cagaagagcg tccggcagct
     4261 ggtgcggtta tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg
     4321 gaactggaaa gcgcagcgct gaatgcgcgt ctggcggcgg aaacgattga tgtctccctg
     4381 ccaggtcgtc gcattgaaaa cggcggtctg catccggtta cccgtaccat cgaccgtatc
     4441 gaaagtttct tcggtgagct tggctttacc gtggcaaccg ggccggaaat cgaagacgat
     4501 tatcataact tcgatgctct gaacattcct ggtcaccacc cggcgcgtgc tgaccacgac
     4561 actttctggt ttgacgctac ccgcctgctg cgtacccaga cctctggcgt acagatccgc
     4621 accatgaaag cccagcagcc accgattcgt atcatcgcgc ctggccgcgt ttatcgtaac
     4681 gactacgacc agactcacac gccgatgttc catcagatgg aaggtctgat tgttgatacc
     4741 aacatcagct ttaccaacct gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag
     4801 gaagatttgc agattcgctt ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa
     4861 gtggacgtca tgggtaaaaa cggtaaatgg ctggaagtac tgggctgcgg gatggtgcat
     4921 ccgaacgtgt tgcgtaacgt tggcatcgac ccggaagttt actctggttt cgccttcggg
     4981 atggggatgg agcgtctgac tatgttgcgt tacggcgtca ccgacctgcg ttcattcttc
     5041 gaaaacgatc tgcgtttcct caaacagttt aaataaggca ggaatagatt atgaaattca
     5101 gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga tagcgatacg ctggcgaatc
     5161 aaatcactat ggcgggcctg gaagttgacg gtgtagaacc ggttgccggt agcttccacg
     5221 gcgtggtcgt tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg
     5281 tgacaaaagt gaatgtcggc ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact
     5341 gccgtcaggg cctgcgtgtg gcggtagcta ccattggtgc tgttctgccg ggtgatttca
     5401 aaattaaagc ggcgaaactg cgtggcgaac cgtctgaagg gatgctgtgc tccttttctg
     5461 agctgggaat ttctgacgat cacagtggca ttatcgaact gcctgcggat gcgccgattg
     5521 gcaccgacat ccgcgaatac ctgaaactcg atgataacac catcgaaatc agcgtgacac
     5581 caaaccgtgc cgactgctta ggtatcattg gtgttgcgcg tgacgttgcc gtgctgaatc
     5641 agctgccgct ggttgaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgttgc
     5701 cgattacagt cgaagcgccg gaagcctgcc cgcgctatct tggccgtgtg gtaaaaggca
     5761 ttaacgttaa agcgccaact ccgctgtgga tgaaagaaaa actgcgtcgt tgcgggatcc
     5821 gttctatcga tgcagttgtt gacgtcacca actatgtact gctcgaactg ggccagccga
     5881 tgcacgcttt cgataaagat cgcattgaag gcggcattgt ggtgcggatg gcaaaagagg
     5941 gcgaaacgct ggtgctgctt gatggcactg aagcgaagct gaatgctgac actctggtca
     6001 tcgccgacca caacaaggcg ctggcgatgg gcggcatctt tggtggcgaa cactctggcg
     6061 tgaatgacga aacgcaaaac gtcttgctgg aatgcgcttt cttcagcccg ctgtctatca
     6121 ccggtcgtgc tcgtcgtcat ggcctgcata ctgatgcgtc tcaccgttat gagcgtggcg
     6181 ttgatccggc actgcagcac aaagcgatgg aacgtgcgac ccgtctgctg atcgacatct
     6241 gcggtggtga ggctggtccg gtaattgata tcaccaacga agcaacgctg ccgaagcgtg
     6301 caaccatcac tttacgtcgt agcaaactgg atcgcctgat cggccatcat attgctgatg
     6361 aacaggtaac tgacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgagt
     6421 ggcaggcagt tgcgccgagc tggcgtttcg atatggagat tgaagaagat ctggtcgaag
     6481 aagtcgcgcg tgtttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga
     6541 ttatgggtac tcaccgtgaa gctgacctgt cgctcaagcg cgtgaaaacg ctgctcaacg
     6601 acaaaggcta tcaggaagtg atcacctaca gcttcgttga tccgaaagtg cagcagatga
     6661 tccatccagg cgttgaagcc ttactgctgc caagcccgat ctctgttgaa atgtcagcaa
     6721 tgcgtctttc tctgtggacc ggcctgctgg caaccgtggt gtacaaccag aaccgtcagc
     6781 agaaccgtgt gcgcattttc gaaagcggtc tgcgtttcgt accagatact caggcaccgt
     6841 tgggcattcg tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc
     6901 actggaacct ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg
     6961 ttctcgacct gaccggtaaa ctgaatgagg ttgagttccg tgcagaagcg aatccggcac
     7021 tgcatccggg gcaatccgca gcgatttatc tgaaagggga acgtattggt tttgttggag
     7081 ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg tcgcactctg gtgttcgaac
     7141 tggagtggaa caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt tctcgcttcc
     7201 cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt
     7261 tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt
     7321 accgcggtaa gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag
     7381 ataccagccg tacactcgaa gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg
     7441 cattaaaaga gcgattccag gcatcattga gggattgaac ctatggcgct tacaaaagct
     7501 gaaatgtcag aatatctgtt tgataagctt gggcttagca agcgggatgc caaagaactg
     7561 gttgaactgt ttttcgaaga gatccgtcgc gctctggaaa acggcgaaca ggtgaaactc
     7621 tctggttttg gtaacttcga tctgcgtgat aagaatcaac gcccgggacg taacccgaaa
     7681 acgggcgagg atattcccat tacagcacgg cgcgtggtga ccttcagacc cgggcagaag
     7741 ttaaaaagcc gggtcgaaaa cgcttcgccc aaagacgagt aatctgatct aactaaaaag
     7801 gccgctctgc ggcctttttt cttttcactg tcgaagagtc accgtaaaat caacgccatg
     7861 acacttcagc agaacggata ccatgctgac acttgcccgc caacaacagc gacaaaatat
     7921 tcgctggtta ttatgcctgt cagttttgat gctgctggcg cttctcttaa gcctttgcgc
     7981 cggtgaacaa tggatctcgc caggtgactg gtttactcct cgtggcgaac tgttcgtctg
     8041 gcaaattcgc ctgccacgta cgctggctgt attgctggtt ggtgcggcgc tggctatatc
     8101 cggcgctgta atgcaggcgt tgtttgaaaa tcctctggca gaacctggac tacttggcgt
     8161 ctctaacggc gcaggcgtgg ggcttatcgc cgcggtattg cttgggcaag ggcaactccc
     8221 caactgggcg ctagggctgt gtgcgattgc tggcgcgctt atcatcactt taatactctt
     8281 acgtttcgcc cgtcgtcatc tttcgaccag tcggttattg ctggctggcg ttgcattagg
     8341 gattatctgt agcgcactaa tgacgtgggc tatctacttt tccacctctg ttgatttacg
     8401 tcagctgatg tactggatga tgggcggttt tggcggcgta gactggcggc aaagctggct
     8461 gatgctggca ttgatccccg tgttgttgtg gatctgttgt cagtccaggc cgatgaatat
     8521 gttagcactt ggcgagatct cggcgcggca actgggttta cccctgtggt tctggcgcaa
     8581 tgtgctggtg gccgcgaccg gctggatggt tggcgtcagt gtggcgctgg cgggtgctat
     8641 cggctttatt ggtctggtga tcccacatat tcttcggttg tgtggtttaa ccgatcatcg
     8701 cgtattactt cccggctgcg cgctggcagg ggcgagcgca ttgctgctgg ccgatattgt
     8761 agcgcgcctg gcattagctg ccgcagagct gcctattggc gtggtcaccg caacgttggg
     8821 tgcgccggtg tttatctggt tattgttaaa agcaagacgt tagccgcaaa aagacggtct
     8881 atgattaaaa gctaagattt ttactgacca cacccaggag aaacgatgca agattccatt
     8941 ctgacgaccg tagtgaaaga tatcgacggt gaagtgacca cgctggagaa gtacgctggt
     9001 aatgtgctgt tgattgtcaa tgtcgcctca aagtgtggct taacgccgca atatgagcag
     9061 ttggagaata ttcagaaagc ctgggccgat cgaggttttg tggtgctggg attcccgtgc
     9121 aaccagtttc tggaacaaga accgggcagc gatgaagaga ttaaaactta ctgtaccacc
     9181 acatgggggg tgacgttccc gatgttcagt aagattgaag ttaatggcga aggacgccat
     9241 ccgctgtatc aaaaattgat tgccgcagcg ccgaccgcag tcgcgccgga agagagcgga
     9301 ttctatgccc gtatggtcag caaaggtcgt gcaccgctgt accctgatga tattttatgg
     9361 aattttgaga aatttttagt tggcaggggg acgggttggt catccagcgt ttttccccgg
     9421 atatgacgcc tgaagatccc attgtgatgg aaagcattaa actggcgttg gcaaaataat
     9481 gtctattgtg atgcagttac aggatgttgc ggaaactacc cgcctggggc cgctttctgg
     9541 cgaggttcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg gtaagagtac
     9601 cttgcttgca cgaatggccg gaatgaccag cggtaaggga agcattcagt tcgcggggca
     9661 accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctacc tttcacaaca
     9721 gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc agcacgataa
     9781 aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg acaaactcgg
     9841 acgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt
     9901 gctgcaaatc acaccacaag ccaatcccgc aggtcaattg ctgcttcttg atgagccgat
     9961 gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg ctctgtgtca
    10021 gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc gtcatgcgca
    10081 tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag aagaggtgct
    10141 cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg atatcgaagg
    10201 tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc gggtaattac
    10261 gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt
    10321 attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc gccgccaaat
    10381 gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct acaaagctgg
    10441 catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg ttcgggattt
    10501 gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc cccgagaaac caaagaacaa
    10561 gcctctatcg gcacgcaaat tgataaagac gagttgctgc ctggtgacct ggtctttttc
    10621 aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa caaccaattt
    10681 atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt ctattggcag
    10741 aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa aatggacggc gatgatacat
    10801 cgccgtcc
//
LOCUS       DAZFAH010000154        10696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFAH010000154 DAZFAH010000000
VERSION     DAZFAH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.155"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..832
                     /gene="yghS"
                     /locus_tag="ACHJWB_003647"
     CDS             119..832
                     /gene="yghS"
                     /locus_tag="ACHJWB_003647"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4315217.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            864..1622
                     /locus_tag="ACHJWB_003648"
     CDS             864..1622
                     /locus_tag="ACHJWB_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315218.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1668..3017
                     /locus_tag="ACHJWB_003649"
     CDS             1668..3017
                     /locus_tag="ACHJWB_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO4315219.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKLRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGDKS"
     gene            3017..3841
                     /locus_tag="ACHJWB_003650"
     CDS             3017..3841
                     /locus_tag="ACHJWB_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315220.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3853..4413
                     /locus_tag="ACHJWB_003651"
     CDS             3853..4413
                     /locus_tag="ACHJWB_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4315221.1"
                     /translation="MSSRLIIALIIMLLAPGGQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4444..5514
                     /gene="lptF"
                     /locus_tag="ACHJWB_003652"
     CDS             4444..5514
                     /gene="lptF"
                     /locus_tag="ACHJWB_003652"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4315222.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5511..6590
                     /gene="lptG"
                     /locus_tag="ACHJWB_003653"
     CDS             5511..6590
                     /gene="lptG"
                     /locus_tag="ACHJWB_003653"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4315223.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6626..7798)
                     /locus_tag="ACHJWB_003654"
     CDS             complement(6626..7798)
                     /locus_tag="ACHJWB_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4315224.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7798..8046)
                     /locus_tag="ACHJWB_003655"
     CDS             complement(7798..8046)
                     /locus_tag="ACHJWB_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4315225.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNALGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKPPAQLMETLLPWLENK"
     gene            complement(8078..8992)
                     /locus_tag="ACHJWB_003656"
     CDS             complement(8078..8992)
                     /locus_tag="ACHJWB_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4315226.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8989..10677)
                     /locus_tag="ACHJWB_003657"
     CDS             complement(8989..10677)
                     /locus_tag="ACHJWB_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO4315227.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2558 a         2671 c         2806 g         2661 t
ORIGIN      
        1 tttatgaaag cgtaattcag gtcaatgtca caattaacca ttgtcacaat aaggttgaac
       61 ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg ggtttttcgt
      121 gaatcaacgt aatatgtcaa taattaattc cacgccagtg cgtgttattg ccattgtagg
      181 atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag
      241 aatgccaaca gaacacattt atctcgggca atcgtccggg cgaattggcg aatggatttc
      301 acagctccct gttattggcg caccttttgg gcgttatctg cgaagtaaag cggcacatgt
      361 gcacgaaaaa ccctcaacac cgcctggcaa tattactgct ctggttatct atctgctttc
      421 ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag gctttctgct
      481 catcaccgac cgctacccgc aggttgaagt gccgggtttt cgctttgatg gcccgcaatt
      541 ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg agctgaagct
      601 gtaccaatgg atggcatctt atttgcccgt attgttgatt cgtcttggca ttgatgaaca
      661 aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa aaatcgccgt
      721 tacgccgcaa ctgacattca atggcgcaaa gatccttgaa ctggatgggc gacaccccgc
      781 cgatgaaatt ctgcaagcgt cactacgcgc aattcacgcc gcgctttcct gatcaccaaa
      841 atggataggg gatgatttaa cgaatggatg cactacaaac tcaaactgtt aatagtacaa
      901 ccgcgccgca gcccaactac attccggggc tgattgcggt ggtcgggtgt gatggcaccg
      961 gtaaatccac actgaccacc gacctggtga aatcgctgca acaacactgg caaaccgagc
     1021 ggcgctatct ggggctgctc tccggcgaag acggcgacaa aatcaaaaga ttgccgttgg
     1081 ttggcgtctg gctggaacgg cgactggcgg ccaaatcctc gaaaacccaa agcatgaaaa
     1141 ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg cgaagaatgg
     1201 cgaatctacg caaggttcag cgactggcgc aaagtggcgt tctggtggtc agcgatcgct
     1261 tcccgcaggc tgaaatttcg ggcttttatt atgatggacc ggggattggc gtcgaacgtg
     1321 cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gcgtttatac caacaaatgg
     1381 cgcaatatcg cccggaatta attattcgcc tgggcattga tattgagact gccatctccc
     1441 gcaagcctga ccatgactat gccgagctgc aggacaaaat cggcgtcatg tcgacgattg
     1501 gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc gaagtgctgg
     1561 aacaggcaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga agtttaactt
     1621 aaggctggcg ggatactttt tactatcaac gataaggtca ggctgaattg gcgggtttta
     1681 acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt
     1741 taggctccag taatgtcgtg agcgccttgc tgggtctgtt ggcgctctcg tgtgccggta
     1801 aagggatgac gcccgccatg tttggcgtac tggtgattgt gcaatcgtac gccaagtcga
     1861 tcagcgattt tattaagttt cagacatggc aactggtggt tcagtacgga acaccagcat
     1921 taaccaacaa taatccgcag caattccgca atgtcgtctc attttccttc tcgctggata
     1981 tcgtcagcgg cgcggtggcg attgtcggtg gcattgcctt actgccattc ctttcccatt
     2041 cattaggtct ggatgaccaa agtttttggc tggcagcgct ctattgcacg ctcattcctt
     2101 caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttc gatttaattg
     2161 ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgta gcctggtatt
     2221 ttgactttgg ttttgcgggt tttgttattg cctggtacgt gtcgaatctg gttggcggca
     2281 ccatgtactg gtggtttgcc gcgcgcgaat tacgccgccg aaatatccat aatgccttca
     2341 aattgaatct gtttgagtct gcccgacaca tcaaaggcgc gtggagtttt gtctggtcaa
     2401 ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg ctggtgggga
     2461 tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc ttcgacgccg
     2521 ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc
     2581 cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg ggcggaatcg
     2641 gtattctggt ggcgctcgcg gtgtttatcg tcggcaagcc gctcatttcg ctggtgtttg
     2701 gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca attgtgattt
     2761 cgatgctggg cttccctcag gagtcattgc tattgatggc gggaaaactg cgcgcttttc
     2821 tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg ttctgtcatc
     2881 tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg gacgtggcgc
     2941 tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt tataacgcag
     3001 ccggagataa atcctgatga tgagaaaata tttcccgctt gaggcgagcg agcgtctatt
     3061 tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc cgactattgc
     3121 actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc aattctggct
     3181 caatggcgtt gatcgtcagg aattactgcg tttagttcgc aaacaagcca aaggcgatga
     3241 gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca agcacctgcg
     3301 ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg gcaaaacgac
     3361 cgtttttctg gggcgttttc aggacggctt tcgtaacggt aagaaaaata ttgtgtcgta
     3421 ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg tgaactattc
     3481 attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc aaaagcaaat
     3541 gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt
     3601 gcgaaaaatc atcagccaac aggtttctta ttacgacacc ttaagatcgc ttgatcctga
     3661 aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc tgatgggaga
     3721 taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatg atgcaccgtt
     3781 ggcgctggat agcgatattc gtcagcgctt agagctgctg atttcgcgtt ttccgctata
     3841 gccggagcaa tgatgtcatc tcgtttgatt attgcgttga ttatcatgct cttagccccg
     3901 ggtgggcagg cacacaattt cgtcactggc aaaacggtta cccctgttta tatccaggaa
     3961 ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg gaacagcaca
     4021 caattagcgg gaaaagtccg cattattcag tatatcgccg ggcgtaagtc ggcgaagaaa
     4081 aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga tcgttttcag
     4141 cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt tgttgttggc
     4201 aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcattattga cggcaatgga
     4261 cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt gctgaataaa
     4321 gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt agatcatgtc
     4381 attgcgctgg cgcagaaact gatcaatgaa taaaaaattc aaaggattga gggcgttagt
     4441 gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt gctgattatc
     4501 gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct gaccgaagcg
     4561 gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa agtgctgatt
     4621 gcactggaga tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac gctagggcag
     4681 atgtataccg actcggaaat taccgctatc tctgcggcgg ggggcagtcc gggacgcttg
     4741 tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg
     4801 tatggtcgtc cgtgggctta tacgcagatt tatcaactgg agcagcagtc gcagtcggag
     4861 ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg acgaatgatc
     4921 ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat ttatacttct
     4981 actgccaatc gaacccgcat tttccgcgcc cgttcggttg atgtggttga cccatcaccc
     5041 gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca tcaggggcgt
     5101 gacgacaacg aacagatcta ccgtaatctg caattacatc tgaatccgct ggttcaaagc
     5161 cctaacgtca aacgcaaagc gaaatcggtc acggagctgg cgcgctccgt ctttcctgcc
     5221 gatcatgccg aactgcaatg gcgacaaagc cgtggtctga cagcattgtt gatggcgctg
     5281 ctggccattt cattaagtcg ggtaaaaccg cggcaagggc gattttcaac gttattgcca
     5341 ctgacgttgc tgtttattgc cattttttat ggcggcgacg tctgccgtac gctggtggct
     5401 aacggcgcga ttcccctcat tcctggtttg tggttagtgc ccggactcat gctaatgggc
     5461 ctgctgatgc tggtcgcacg cgacttctct ttgctgcaga aattttcccg atgaatgttt
     5521 tcagtcgcta tttaatccgt catctctttc tcggttttgc cgccgccgca gggctattgc
     5581 tgccgctttt taccaccttc aacttgatta acgaactgga tgatgtcagc ccgggcggtt
     5641 atcgctggac tcaggcggtg ctggtggtgc taatgacctt accgcgcaca ctggtcgaac
     5701 tttcgccgtt tatcgcgtta ttgggtggta ttgttggact ggggcagtta tcgaaaaaca
     5761 gtgagcttac cgccattcgc agcacggggt tttctatctt ccgtattgca ctggtggcgc
     5821 tggttgccgg gatattgtgg actgtttcgt taggcgcgat agatgagtgg gtggcgtcgc
     5881 cattgcagca acaggcgttg caaatcaaat cgaccgccac cgcgttgggg gaggacgatg
     5941 acattaccgg caatatgctg tgggccaggc gtggtaatga atttgtgacg gtgaaatcgc
     6001 tgaacgagca gggccagccg gtgggcgtgg agatatttca ttatcgcgac gatctctcgc
     6061 ttgaatccta catttatgca cgcagtgcca ccattaaaga cgacaaaacg tgggtcctgc
     6121 atggtgtgaa tcataaaaaa tggctgaatg gcaaagaaac gctggaaaca ttagataatc
     6181 ttgcctggca atcggccttc accagtatga atcttgaaga gttatcaatg ccggggaata
     6241 ctttttctgt ccgtcagctt aatcattaca tccattattt gcaggaaacc gggcaaccca
     6301 gcagcgaata ccgccttgca ctgtgggaaa aactggggca acccatcctg accctggcga
     6361 tgattttgct ggctgtgccg tttaccttta ctgccccgcg ttcgcctggg atgggtagcc
     6421 gtctcgctgt aggcgtcatc gtgggcttac tcacctggat cagctatcaa atcatggtta
     6481 atctgggatt gctatttgcg ttaagcgcac ctgttaccgc gctcggttta ccggtagcgt
     6541 ttgtgttggt ggcgttgagc ctggtgtatt ggtatgacag acaacattaa acccgccgtg
     6601 gcgcaaacac cacggcgggc tcacattact gcttcagagt agcaaatgcc tgaatgatgg
     6661 catcgatttg ctcacgactg tgggcagcat ttacgctaca gcgcagcagc gtgatgcctg
     6721 caggcgcggc aggcggcagg ataagattca catacacgcc cagcgagata agctcgcgcc
     6781 agatgcgtaa accttcctct ttcgaaccga ttattactgg cactaccggg ctaatatgtg
     6841 aacttaactc ataacccagt ttttgcagac cgtcatacag atggttggcg ttatccatca
     6901 gcttttgccg cagctctggt tgtgtggcaa tagttcttaa cgacgagcgc acggtcgcaa
     6961 tgcaggacgg agaaggagag gcggtaaaaa tatacgggcg gctgccgtag cgcagcactt
     7021 ccatcgcttc cgaaccgaca gcaaaaccgc caatcgaggc cagactttta ctgaaagtac
     7081 caacgataat atccacatca tcttcaacgc cgacagcttc agccagtccg cgccctgttg
     7141 cgcctaacac gccaaacgaa tgcgcttcat cgacaatcaa ataaccaccg agacgacgtt
     7201 tgatatcaac aatttccgcc agcggcgcaa cgtcaccgag catgctgtag atgccctcaa
     7261 cgatgataat cgcctctttc gcccgttcac cgaggcgaac catgcgccgt tccagatctt
     7321 tagcatcgtt atggcggaaa cgaataattt ccgcgccacc gagagaacag gcgtcataaa
     7381 tactggcgtg gctgtcggca tccagtaaca ccacggcgtt atggtcggca agtgcgctga
     7441 tgacgcctaa attagcggta taaccggttg aaaaaacgat agcggtaggg cgattgaaaa
     7501 actcggcaat ctctttctct aacgccaggt gcggcgcata actgccgttt gccatacgag
     7561 aaccggtggt gccggtgcct tgggccgcta acgctgcctg accttcgctg atggcatcat
     7621 gattaaaggt taaaccgaga taattattgg tcccggccag aatcactttt ttattgccga
     7681 tccggccttc ggtggcggaa tacacttcat caatacaggt gccaaacggt gttaacccgt
     7741 tgtcactaaa ttgcagtcgc tcgccagcaa ggcgcgcata tttatcgtat agccccatta
     7801 tttattctcc agccatggaa gtagagtttc cattaattgc gcagggggtt tgacatccag
     7861 cagaatatta ataggaatgg aaatatcgaa tctgtcttcc agcatcatta agagatccat
     7921 cactttgatc gactccaggc caagagcgtt gacgagatcg ctgtcgggct taatttccac
     7981 accgttttcg accaaatcct gtaaacagga aaggatgtaa tccattacta tttcacgatt
     8041 taccatagaa acgttaacac acctttaaac aagacaatca aaatagcccg ttgcgtaatg
     8101 cgtgttccag gctaatcccg ggaaaccaat taatatcttc agaaatacgg ttattacttg
     8161 ccgaccagtc ggcgtgggtt aattcacgaa ttttcgagcg ggtcagcata ggttctttac
     8221 ctgccaggcg actcaacgcg gtactgatat ccgcaaggca ggtgagtacc ggcagaggaa
     8281 taccaaccat tcgcacggaa ccgcaacgga cgttggcggc aagttgctgt atgcgttgcc
     8341 agtcatagcc gccagcgacg ccatcgcata attcataggt ttgcgtctgt acagtttcgg
     8401 cgcttaacca ctgacccact gcttgcgcaa aatcggtgac gtgcaggaaa gagagctgtg
     8461 tatctggtgc accaagtcgt ggcagcaggc cgcgcagcat ccagtcaaac agcggtttta
     8521 actctttatc gccgggacca tacacggctg tcgggcgaaa aacgcccagc gtaatttcgt
     8581 cagccattgc agtcagccgt tgttcggcga tgtgtttgga atttgcgtac caggagagct
     8641 cgggatggcg cgccgccagc gaagagataa acagaaaacg ttggcaaaag cagctctctt
     8701 ttgctgcttg catcaggcgc aggctgccgt caacgttaca gcgggtgaaa atctcttctt
     8761 tgtgcccgcg cacttgcccg gcgcaatgga ccactgcgct ggctccggca accagcttgc
     8821 taagtgaatg agtatcttcc agcgaaccgc gcacccaggt gagattatca ttgacgtgag
     8881 cgcgggcagt acgcgtcaat gcgcgaacgt gaaagccgcg ggcgagcagg ttatcgataa
     8941 tatatttacc gataaacccg gtagcgcccg tcaccgcgac agtttgattc atgccaggga
     9001 ttcctgcaca ttaagactgg cagcataagc cttctgataa cgttttttcg cttccgcacg
     9061 ggcaggcttg ccggaggacg ttcggggaat actgtggggc ggcaacagat cgatagccgc
     9121 ggtcacgcca aattcgcttt ggatccgtgc cgccagcgcg tggataagct gcccgcgacg
     9181 ttcttcgtcg ctgatccgac actggatctg caaaatgatt ttttcctggg cggtaacaaa
     9241 agcaatcgca tcgccagaat gaatttccgg ttcttgttcc gcaatatatt caatatcctg
     9301 cggccagata ttacggccac gaataataat cagatcttta atgcgtccgg tgacatacag
     9361 ataaccgtcc agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc
     9421 gtcttgcgaa acctggtcgc caaagtaacc gctcatcagg ctggggccgg agatgcaaat
     9481 atggcctacc acacgttccg cgaccggcat acctgcttca ttgcggattt caatgccatg
     9541 ttccggcaac gctttgccgc agttgacgaa agtcgatacg gcgcgtgtct ctgcacccgg
     9601 cgcgacggct ttaccctgat attcgaggat gtcgcgatcc acttcgttaa ccacaacccc
     9661 ggaggcttca tcagagaagc tgacagccag cgcattttct gccagtccgt agcacggcat
     9721 gaaagtttta ttgtcaaagt taacctgacg gaaacattca gcgaattgat ggagttgttc
     9781 tgcggagatg ggttctgcac caataccagc gacgcgccag caggaaagat ccagttcagc
     9841 gagatctttt tcattcacgc ggcgctggca caattcatag ccaaacggcg gcgcaacgga
     9901 aacggtgccg cgatttttac tgatcaattt aagccattgc agaggacgca tggcaaaatc
     9961 ctgagtgcgc aaataatcta ctgaaagctg cgtggcgacg ggggtcagga gaaagccgac
    10021 cagtcccata tcatggtaga aaggcagcca ggagacgcag cggtcgccag ggcgtaattt
    10081 aatgccgtcg tggcttatag cacgtagatt agccatcact tcgcgatggg tgataatgac
    10141 gccacgggga aaacgggtgc tgccggaggt gtactggagg taggcgatat cgttcggaac
    10201 tggacgctgg agcgcaacat cggcttccgg taatgcctta aaccaggcgt ggcttaaaac
    10261 atgtaattcg gggttgttat gcgtcgcggc attgaccagt ggcaaccact catcaccagt
    10321 gataatggct gcgggctggc aacttgccag taaaccctgg agtttggcgc tccaggaatc
    10381 ccgctgaccg acgcccattg gaatcgccaa cgggacggcg actaagccag catactgaca
    10441 ggcaaaaaaa gcctctacaa acccgctact cgtttcggca atcagcgcca cgcgttcgcc
    10501 ttttttcagg ttcagcgata acaaccgctt cgcaccagct tcggcacgcg tttttaatgt
    10561 ctgatattcc agttgatctt caagttggca acgtctgtca taaaaattca ttccggcgct
    10621 actcagagcg gcgtagtcca aagcatcaac cagcgttgga aaatcggcat agcgcatagg
    10681 tagggaatgc gtaaag
//
LOCUS       DAZFAH010000155        10668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFAH010000155 DAZFAH010000000
VERSION     DAZFAH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.156"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..1191
                     /gene="yghO"
                     /locus_tag="ACHJWB_003658"
     CDS             49..1191
                     /gene="yghO"
                     /locus_tag="ACHJWB_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO4315228.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1198..1962)
                     /gene="glcC"
                     /locus_tag="ACHJWB_003659"
     CDS             complement(1198..1962)
                     /gene="glcC"
                     /locus_tag="ACHJWB_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO4315229.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2213..3712
                     /gene="glcD"
                     /locus_tag="ACHJWB_003660"
     CDS             2213..3712
                     /gene="glcD"
                     /locus_tag="ACHJWB_003660"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO4315230.1"
                     /translation="MSILYEESLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3712..4764
                     /gene="glcE"
                     /locus_tag="ACHJWB_003661"
     CDS             3712..4764
                     /gene="glcE"
                     /locus_tag="ACHJWB_003661"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO4315231.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4775..5996
                     /gene="glcF"
                     /locus_tag="ACHJWB_003662"
                     /pseudo
     CDS             4775..5996
                     /gene="glcF"
                     /locus_tag="ACHJWB_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6001..6405
                     /locus_tag="ACHJWB_003663"
     CDS             6001..6405
                     /locus_tag="ACHJWB_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO4315232.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6427..8598
                     /gene="glcB"
                     /locus_tag="ACHJWB_003664"
     CDS             6427..8598
                     /gene="glcB"
                     /locus_tag="ACHJWB_003664"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO4315233.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQPEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8954..10636
                     /gene="glcA"
                     /locus_tag="ACHJWB_003665"
     CDS             8954..10636
                     /gene="glcA"
                     /locus_tag="ACHJWB_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO4315234.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2473 a         2705 c         3010 g         2480 t
ORIGIN      
        1 ctgttgcaac ctgtttttca ccgttaatta tttgttatcc acagagatat ggaatgcgat
       61 ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct
      121 tttccgtcat cactttatcc cgacgatcca aactggatac cccctttatt catagaacgc
      181 aacgagcatt tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg
      241 gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt
      301 gagcgttacg gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag
      361 gtttttgccg cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag
      421 atcagcggtc ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt
      481 gacacaccac cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa
      541 caactgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc
      601 actttctctc cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc
      661 tgcatcaatc gtcagcggtt tgccgaagaa atgcagatcc tgcgtgagat tttcaactct
      721 ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc
      781 gatcaactta aatatctggt gccggacgat atgatctata tcgctgagat tgattctgca
      841 ccctgcgcgt ttattgtcgg cttgccgaac atcaacgagg cgattgccga tctgaacgga
      901 tcgctctttc ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga
      961 actgcgcgag taccgctgat gggcgtgcgc gacgagtatc agttcagtcg catcggcccg
     1021 gtgattgcgc tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat
     1081 gcgctggaga tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc
     1141 attggggcgg agccgtacaa gcgttaccga ttgtatgaaa agcagattta ataccagcta
     1201 actcaggttc atctccagcg gcaccgccga gcgaatcaaa tggtggcctt ccagctcgat
     1261 atcgtggaga ttctttttca cggtccgcac atgatcgcgt gctgcgcgct gggcgacgtg
     1321 cggcagccgc tgcaacaccg cgttgtagat cctcgcatgc tggcgatcga tctgcttttt
     1381 ttgctgtggt cgatggtaga gattatttac gctggcaaac actgaattaa acatcagatc
     1441 ggtcaatgat tgcagcgtaa acaccagcac ctgattgtga gaagcctgac aaatggcgag
     1501 atggaaagcg tgatccaact gcgcatgttc gatcagcgaa atctctttgt tgttctcact
     1561 ggcggcgagc attttttcat aacagcgggt tatcacaaca aaatcagcct gcgttcccag
     1621 cgttgccgcc agccttgccg attcgccctc cagtaatgcg cgaacgtcga gcagatcgta
     1681 cagcgttcgc ggctgcgtac tgaacagatg gatcagcggg ctggtgtcct gcacccgatt
     1741 aagccgtgcg acacgagaat cgcgaccctg cgccgtttca ataatcccgc gcccgcgcag
     1801 cacggtcagc ccttcacgca gtgcggagcg tgagaagccg agcttttcac acagtcgacg
     1861 ttccgaggga agtggctgac cgaccttcag tacgccgtcg ataattaacc gttcgatact
     1921 ctctgcaacc acttcgcaaa tagggcgacg ttcatctttc attcccggac ctcgtgcaca
     1981 ggtaggacca atttttctgt tcttaactcg caaaacacgc acatcacgta agtgattatg
     2041 ttacatcaat ttaacattga gttaaccaag gcaaggtcac agagctggaa aaaaaatggt
     2101 ctgaccggta gctaaagaga tagacgaaaa cgaaaagccc gcttaataac tgttcacaga
     2161 agcagcgcgc aaaaatcagc tgccacacaa cacaacaaag cgaagcctac tcatgagcat
     2221 cttgtacgaa gagagtcttg atggcgcttt acccgatgtc gaccgcacat cggtactgat
     2281 ggcactgcgt gagcatgtcc ctggacttga gatcctgcat accgatgagg agatcattcc
     2341 ttacgagtgt gacgggttga gcgcgtatcg cacgcgtcca ttactggttg ttctgcctaa
     2401 gcaaatggaa caggtgacag cgattcttgc tgtctgccat cgcctgcgtg taccggtggt
     2461 gacccgtggt gcaggcaccg ggctttctgg tggcgcgctg ccgctggaaa aaggtgtgtt
     2521 gttggtgatg gcgcgcttta aagagatcct cgacattaac cccgttggtc gccgcgcgcg
     2581 cgtgcagcca ggcgtgcgta acctggcgat ctcccaggcc gttgcaccgc ataatctcta
     2641 ctacgcaccg gacccttcct cacaaatcgc ctgttccatt ggcggcaacg tggcagaaaa
     2701 tgccggcggc gtccactgcc tgaaatatgg tctgaccgta cataacctgc tgaaaattga
     2761 agtgcaaacg ctggacggcg aggcgctgac gctgggatcg gacgcgctgg attcacctgg
     2821 ttttgacctg ctggcgctgt tcaccggatc ggaaggtatg ctcggcgtga ccaccgaagt
     2881 gacggtaaaa ctgctgccga agccgcccgt ggcgcgggtt ctgttagcca gctttgactc
     2941 ggtagaaaaa gccggacttg cagttggtga catcatcgcc aatggcatta tccccggcgg
     3001 gctggagatg atggataacc tgtcgatccg cgcggcggaa gattttattc atgccggtta
     3061 tcccgtcgac gccgaagcga ttttgttatg cgagctggac ggcgtggagt ctgacgtaca
     3121 ggaagactgc gagcgggtta acgacatctt gttgaaagcg ggcgcgactg acgtccgtct
     3181 ggcacaggac gaagcagagc gcgtacgttt ctgggccggt cgcaaaaatg cgttcccggc
     3241 ggtaggacgt atctccccgg attactactg tatggatggc accatcccgc gtcgcgccct
     3301 gcctggcgta ctggaaggca ttgcccgttt atcgcagcaa tatgatttac gtgttgccaa
     3361 cgtctttcat gccggagatg gcaacatgca cccgttaatc cttttcgatg ccaacgaacc
     3421 cggtgaattt gcccgcgcgg aagagctggg tgggaagatc ctcgaactct gcgttgaagt
     3481 tggcggcagc atcagtgggg aacatggcat cgggcgagaa aaaatcaatc aaatgtgcgc
     3541 ccagttcaac agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt ttgaccccga
     3601 tggtttgctg aaccctggga aaaacattcc cacgctacac cgctgtgctg aatttggtgc
     3661 catgcatgtg catcacggtc atttaccttt ccctgaactg gagcgtttct gatgctacgc
     3721 gagtgtgatt acagccaggc gctgctggag caggtgaatc aggcaattag cgataaaacg
     3781 ccgctggtga ttcagggcag caatagcaaa gcctttttag gtcgccctgt caccgggcaa
     3841 acgctggatg tgcgttgtca tcgcggcatt gttaattacg acccgaccga gctggtgata
     3901 accgcgcgtg ccggaacgcc gctggtggcg attgaagcgg cgctggagag cgccgggcaa
     3961 atgctcccct gcgagccgcc gcattatggt gaagaagcca cctggggcgg gatggtcgcc
     4021 tgcgggctgg cggggccgcg tcgcccgtgg agcggttcgg tccgcgattt tgtcctcggc
     4081 acgcgcatca ttaccggcgc tggaaaacat ctgcgttttg gtggcgaagt gatgaaaaac
     4141 gttgccggat acgatctctc acggttaatg gccggaagct acggttgtct tggcgtgctc
     4201 actgaaatct cgatgaaagt gttaccgcga ccgcgcgcct ccctgagcct gcgtcgggaa
     4261 atcagcctgc aagaagccat gaacgaaatc gcccagtggc aactccagcc gctacccatt
     4321 agtggcttat gttacttcga caatgcgttg tggatccgcc ttgagggcgg cgaaggatcg
     4381 gtaaaagcgg cgcgtgaact gctggggggc gaagaggttg cgggtcagtt ctggcagcaa
     4441 ttgcgtgaac aacaactgcc gttcttctcg ttaccaggta ccttatggcg catttcatta
     4501 cccagcgatg cgccgatgat ggatttaccc ggcgagcaac tgatcgactg gggcggagcg
     4561 ttacgctggc tgaaatcgac agccgaggac aatcaaatcc atcgcatcgc ccgcaacgct
     4621 ggcggtcatg cgacccgctt tagtgccgga gatggtggct ttgccccgct atcggctcct
     4681 ttattccgct atcaccagca gcttaaacag cagctcgacc cttgcggcgt gtttaacccc
     4741 ggtcgcatgt acgcggaact ttgaggagca ggctatgcaa acccaattaa ctgaagagat
     4801 gcggcagaac gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat
     4861 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     4921 ggcgcatcta tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg
     4981 agcatctcga tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc
     5041 gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     5101 tgccggagcg aatactgcgc gaaggattgc gccaggtagt gccgcgtccg gctgtcttcc
     5161 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5221 aactgcctgc cgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg
     5281 ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5341 cgcgagtgct ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg
     5401 gcgcggtgga ctatcatctt aatgcgcagg agaaagggct ggcacgggcg cgcaataata
     5461 ttgatgcctg gtggcccgcg attgaagcag gtgctgaggc aattttgcaa accgccagcg
     5521 gttgcggcgc gtttgtcaaa gagtacgggc agatgctgaa aaacgatgcg ttatatgccg
     5581 ataaagcgcg tcaagtcagt gaactggcgg tcgatttagt cgagcttctg cgcgaggaac
     5641 cgctggaaaa actggcagtt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc
     5701 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttgcttcgt cttggattta
     5761 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     5821 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     5881 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc
     5941 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataacaaa
     6001 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     6061 gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6121 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag
     6181 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6241 aacggacgta ccgcgttcgt gactgcgccg ttattaacgt cgctggaagg cggcgtaccg
     6301 gttgttgttg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6361 gatgcgcagg tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6421 taaacaatga gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt
     6481 tttgtggatg aagaagtttt accgggaaca gggctggacg ctgcggcgtt ctggcgcaat
     6541 tttgatgaga tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat
     6601 cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6661 gcggcctata aatctttcct gcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg
     6721 acggtggaaa ccacgggcat tgacagcgaa atcaccagtc aggcggggcc acagctggtg
     6781 gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     6841 tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcggtggt cagcggctac
     6901 gatccgcaac gcggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatctcta
     6961 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta
     7021 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ccccggcgca gtttgtcggt
     7081 taccgtggcg atgccgctgc gctgacctgc attttgctga aaaataacgg cctgcatatt
     7141 gaactgcaaa ttgatgccaa cgggcggatt ggcaaagacg atccggcgca catcaacgat
     7201 gttatcgtcg aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt
     7261 gatgcggaag ataaaatcct gctgtaccgt aacctgctgg gcctgatgca ggggactctg
     7321 caagagaaaa tggagaaaaa cggtcgacaa atcgtgcgta aactgaatga cgatcgtcat
     7381 tacaccgccg ccgatggctc tgaaatttct ctgcacggac gctcgctgct gtttatccgc
     7441 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7501 gaaggcattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7561 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa
     7621 gttgcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat
     7681 accctgaaaa tgggcattat ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt
     7741 atcgctcagg cgcgtaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     7801 gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg
     7861 acgccttgga tcaaagccta cgaacgtaat aacgtgcttt ccggcctgtt ctgtgggctg
     7921 cgcggtaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     7981 tacagccaga agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcgcca
     8041 actgccgcta cgctccatgc gctgcactac caccaaacca acgtacagag cgtgcaagcc
     8101 aacattgccc agcccgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact
     8161 attccggtgg ctgaaaacgc taactggtcg gtggaagaga tccaacaaga gctggataac
     8221 aacgtgcagg ggattctggg gtacgtggtg cgctgggtgg agcaggggat tggttgttca
     8281 aaagtgccgg atattcacaa tgtggcgctg atggaagacc gcgcaacgct gcgtatctcc
     8341 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg
     8401 tcgctggaga atatggcgaa agtggttgat cagcaaaacg ctgacgatcc ggcttatcgt
     8461 ccgatggcgg ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc
     8521 ctcggcgtga aacagccaaa cggctatacc gaaccgttat tacacgcctg gcgtttacgc
     8581 gaaaaagaaa gtcattaata gcgtcaaaaa aagccccggc aagttttatt gtcggggcta
     8641 tgatagtaac tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta
     8701 acaatattaa atacccgtag cgcattacta gcgggcctat aacaacaata tggacatctt
     8761 aacgatactc tgcgcctgcg tgtgcagaac agaaaccctt acccgatata cttgttatgc
     8821 cagaggtagc aattgcgtta gcggcgtact gttgctgtaa agtataacgc ctatatttta
     8881 cagttcagtc ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa
     8941 gagactgaac aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct
     9001 atccgctctg gtcgccctga tcccgataat attcttcttc gttgcactcg cggtattacg
     9061 tctgaaagga catgtcgctg gagcaataac ccttatatta tctatcctga ttgcaatatt
     9121 cgcctttaaa atgccgattg atatggcttt tgctgctgcg ggctatggct ttatttatgg
     9181 attatggcct attgcgtgga ttattgtcgc ggcggtgttc ctctataaat taaccgttgc
     9241 cagcgggcag ttcgatatta tccgcagctc ggttatctcc atcaccgacg atcagcgttt
     9301 gcaggtgtta ctgattggtt tctcctttgg tgcgttgctg gaaggagcgg ctggctttgg
     9361 tgctccggtg gcgattaccg gtgcgctgct ggtgggcctg ggcttcaaac cgttatacgc
     9421 ggcggggttg tgtctgattg ccaacactgc tccggtggcg tttggtgcgt tgggcgtgcc
     9481 gattctggtc gccggtcagg taacgggaat cgatccgttc cacattggcg caatggcggg
     9541 acgccagtta ccgttcctgt cggttcttgt gccgttctgg ctggtagcaa tgatggacgg
     9601 ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt gctgggggaa gctttgctgt
     9661 cactcagttc tttacctcta actatattgg tccggaactg ccggatatta cttcggcgct
     9721 ggtgagtatc gtctcactcg ctttattcct taaagtctgg cggccgaaaa ataccgaaac
     9781 ggcaatcagc atggggcaat ccgcaggtgc gatggtggta aataagccat cttctggcgg
     9841 tcccgtgcct tcagaatata gtctggggca aatcattcgt gcgtggtcac cgtttttaat
     9901 cttaacggtg ctggtcacca tctggaccat gaagccgttt aaagcgttat ttgctccggg
     9961 cggcgcgttt tattcactgg tgattaattt ccagatccct catttgcatc aacaagtgtt
    10021 gaaagcggca cccattgtcg cccaaccaac gccaatggat gcggtattta aattcgaccc
    10081 tctctcggct ggcggcaccg ctatttttat tgcggcgatt atctctatct tcatcctcgg
    10141 cgtgggcatc aagaaaggga ttggcgtctt tgccgaaacg ctaattagtc tgaagtggcc
    10201 gatattgtcg attggcatgg tgctggcgtt cgccttcgtc accaactatt ccggcatgtc
    10261 caccacgttg gcgctggtac tggcaggtac aggcgtgatg ttcccgtttt tctcaccgtt
    10321 cctcggctgg ctgggggtat tccttaccgg ctcggacact tcttcaaacg ccctgtttgg
    10381 ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct gacactctgc tggtggcagc
    10441 aaataccagc ggcggcgtaa ctggcaagat gatctccccg caatctatcg ccgtggcctg
    10501 cgccgcgacg ggcatggtgg gccgagaatc tgaactattc cgctacaccg tgaagcacag
    10561 tctgattttt gccagcgtta tcggcgttat caccctgctg caggcgtatg tgtttaccgg
    10621 gatgttagtc tcgtaaatca ataaagccgg atgatcgtta atatcatc
//
LOCUS       DAZFAH010000156        10570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFAH010000156 DAZFAH010000000
VERSION     DAZFAH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.157"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..1472
                     /gene="dcuC"
                     /locus_tag="ACHJWB_003666"
     CDS             105..1472
                     /gene="dcuC"
                     /locus_tag="ACHJWB_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4315235.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLNPAKSNGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1515..2012)
                     /gene="sspB"
                     /locus_tag="ACHJWB_003667"
     CDS             complement(1515..2012)
                     /gene="sspB"
                     /locus_tag="ACHJWB_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4315236.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2018..2656)
                     /gene="sspA"
                     /locus_tag="ACHJWB_003668"
     CDS             complement(2018..2656)
                     /gene="sspA"
                     /locus_tag="ACHJWB_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4315237.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3051..3443)
                     /gene="rpsI"
                     /locus_tag="ACHJWB_003669"
     CDS             complement(3051..3443)
                     /gene="rpsI"
                     /locus_tag="ACHJWB_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4315238.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3459..3887)
                     /gene="rplM"
                     /locus_tag="ACHJWB_003670"
     CDS             complement(3459..3887)
                     /gene="rplM"
                     /locus_tag="ACHJWB_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4315239.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4106..5233)
                     /gene="zapE"
                     /locus_tag="ACHJWB_003671"
     CDS             complement(4106..5233)
                     /gene="zapE"
                     /locus_tag="ACHJWB_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4315240.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5427..5825
                     /gene="zapG"
                     /locus_tag="ACHJWB_003672"
     CDS             5427..5825
                     /gene="zapG"
                     /locus_tag="ACHJWB_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4315241.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5979..7346
                     /gene="degQ"
                     /locus_tag="ACHJWB_003673"
     CDS             5979..7346
                     /gene="degQ"
                     /locus_tag="ACHJWB_003673"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4315242.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7436..8503
                     /gene="degS"
                     /locus_tag="ACHJWB_003674"
     CDS             7436..8503
                     /gene="degS"
                     /locus_tag="ACHJWB_003674"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4315243.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8566..9504)
                     /gene="mdh"
                     /locus_tag="ACHJWB_003675"
     CDS             complement(8566..9504)
                     /gene="mdh"
                     /locus_tag="ACHJWB_003675"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4315244.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9939..10409
                     /gene="argR"
                     /locus_tag="ACHJWB_003676"
     CDS             9939..10409
                     /gene="argR"
                     /locus_tag="ACHJWB_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4315245.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2606 a         2674 c         2632 g         2658 t
ORIGIN      
        1 acacgacatc cgcaccataa atagacttgc aaaaaatata acatcattgt tttcaatctg
       61 ccgtttatgg gattgaccgt tttcttttga cacggagttc aacaatgttc ggcataatta
      121 tatctgtcat cgtattaatt acgatgggct atttgatcct gaaaaactac aaacctcagg
      181 tggtgctggc tgccgcaggt atcttcctga tgatgtgcgg tgtctggtta gggttcggtg
      241 gtgtactcaa tcccgccaaa agcaacggct acttgatcgt cgatatttat aatgaaatcc
      301 tgcgcatgct gtccaaccgc attgccggat tggggctatc gattatggcg gtgggcggtt
      361 atgcccgcta catggagcgc ataggggcca gtcgcgcgat ggtgagcttg ttaagccgcc
      421 cgttaaaact cattcgctcg ccgtatatta ttctgtcggc aacttacgtc atcggtcaaa
      481 tcatggcgca gtttattacc agcgcctccg gtctgggtat gttgctgatg gtcaccttat
      541 ttccgacact ggtgagtctg ggagtaagtc gtctctctgc ggtggcggtt atcgcaacca
      601 caatgtccat tgagtgggga attctggaaa cgaactccat ttttgcagcc caggtcgcgg
      661 gaatgaaaat tgccacttac ttcttccact accagcttcc ggtcgcctct tgcgtcatta
      721 tctcggtggc gatctcccac tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata
      781 tcaatcacga acaagcagag caaaaagctc tcgataatgt cccgccgctc tattacgcca
      841 ttttacctgt gatgccgtta atcctgatgc tcggctcgct gttcctcgcc cacgtcgggc
      901 tgatgcagtc agaactgcat ctggtggtgg tgatgttact gagtttgacg gtgacgatgt
      961 ttgttgagtt cttccgcaag cataacttgc gcgaaacaat ggacgatgtg caggcgtttt
     1021 ttgacggcat gggtacgcag tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat
     1081 ttgcgaaagg cttaacgacg attggcactg tcgatgcggt tatcaggggt gcggagcatt
     1141 ctggtctggg cggtattggc gtgatgatta ttatggcgct ggtcattgcc atttgtgcca
     1201 ttgtgatggg ctctggcaat gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg
     1261 cagccggact acatgtacca gcggttgtaa tgattatgcc gatgcatttt gccacgacgc
     1321 tagcgcgcgc ggtttcgccg attactgcgg tggtggtcgt tacgtcagga attgcaggcg
     1381 tttcgccttt tgcggtggtg aagcgaacag cgatccccat ggcagtcggt ttcgtggtga
     1441 atatgattgc cacaatcacg ctattttatt aagccattaa aagacaaaac aggccaccag
     1501 ggcctgtttt tgtattactt cacaacgcgt aatgccggtc gaccaccgcg tggtggctgc
     1561 ggaggttcat cgtcaggatg agtgtcatca tcgtgatctg gcttgtcgcc atcaataacc
     1621 gacataacgg tttcgttgtc tgccgatgcc tcttcatcat tcatgatgct ggtatcttca
     1681 tcgtaggcag cttcaggctc aaacattgtg cctgcgccat tttcacgggc gtagatagcc
     1741 agcacggcag ccagcggcac agaaacctga cgcggaatgc cgccaaagcg cgcgttaaag
     1801 cgcacctcat cattcgccag ttccagattg ccgacagcac gcggcgcaat gttgagtacg
     1861 atttgtccgt cacgcgcata ttccatagga acctgcacgc cagggagcgt cacatccacc
     1921 accaggtgcg gcgtgagctg gttatccagc aaccactcat agaatgcacg cagcagatag
     1981 ggacgacgtg gtgttagctg tgacaaatcc atacagatta actccggccc agacgcattt
     2041 cacgttctgc ttcagttaaa gaagcaagga aagagtcacg ctcaaagacg cgggtcatat
     2101 agcctttcag ctctttcgca cccgggccgc tgaactcgat gcccagttgc ggcagacgcc
     2161 acagcagcgg agcaagatag caatcgacca ggctgaactc atcgctcagg aagtacggct
     2221 tctgaccgaa gaccggcgca atcgccagca gttcttcgcg cagttgctta cgtgcggcat
     2281 ctgcttcaga agctgaaccg ttgatgatgg tgttcatcag cgtgtaccag tctttttcga
     2341 tacgatgcat gtacagacgg ctttcaccgc gagctaccgg gtaaacaggc atcagtggcg
     2401 gatgcgggaa acgctcatcc agatattcca taatgatgcg agattcccac agggtcagct
     2461 cacgatccac cagggtcgga acgctctgat tcgggttgag gtcaatcaga tcctgaggcg
     2521 gattgtcctt ttccacgtgt tcgatctcga aacttacacc tttctcagcc agcacaatgc
     2581 ggacctgatg gctatagatg tcagtaggac cggaaaacag cgtcattacc gaacgtttgt
     2641 tggcagcgac agccatgaaa acctccaggt atagtcagaa tttttactgc taccagccac
     2701 caggtggcca gtcagaagtt gtgttaccca gtaaggaact actctctttg ttcgaaaatc
     2761 aaacaaaaaa tgagcaatac ccgacatttg ggcagaaaat tggatgatag tttaccagat
     2821 tttgcgacct ttgtggtgag tcgatgccgg aaatggggaa aaagagatgc gctttagtct
     2881 gaaatagttg acttagtccc ttattggcga tgtggttttt gttttgcctg tctgtcagac
     2941 ggtagcaaaa agcaactttc caatttttac gctgattcag aatttaacca taaaaaaacc
     3001 cgccgaagcg ggttttttcg caaattgttt tctgccggag cagaagccaa ttaacgtttg
     3061 gagaactgcg gacgacgacg tgctttacgc agaccgactt tcttacgttc aacctgacga
     3121 gcgtcacgag taacgaagcc agctttacgc agttcagaac gcagggactc gtcgtattcc
     3181 atcagagcgc gggtgatacc gtgacggatc gcaccagcct gaccagagat accaccacct
     3241 ttaacggtga tgtacaggtc cagtttctca accatgtcga ccagttccag cggctgacga
     3301 actaccatgc gggcagtttc acgaccgaag tactgttcca gagaacgttg gttgattacg
     3361 attttaccgt tgcccggttt gatgaaaacg cgagctgcgg aacttttgcg gcgaccagtg
     3421 ccgtagtatt gattttcagc cattgcctat aatcccgatt agatgtcaag aacttgcggt
     3481 tgctgtgccg cgtggttgtg ctcgttaccc gcgtaaactt tcagtttacg gaacatagca
     3541 cgacccagcg ggccttttgg caacatgcct ttaaccgcga tttcaatcac acgctcagga
     3601 cggcgagcaa tcatctcttc aaaggtcgct tgtttgatac caccgatgtg gccggtgtgg
     3661 tgatagtaca ctttgtcagt acgcttgttg ccggttacag caactttgtc agcgttcaga
     3721 acgatgatgt aatcaccggt atctacgtgc ggagtgtatt ccgctttgtg cttaccgcgc
     3781 aggcgacgag ccagttcagt agccagacgg cccagagttt taccggtcgc gtcaacaaca
     3841 taccagtcgc gttttacggt ttctggttta gctgtaaaag ttttcattaa aagcttaccc
     3901 aataaatagt tacacgttgg tgaacaccca aacgtcttca attgttgagg ttcacacgac
     3961 aaagtccggc aaacctaccc cttcgaatag cctatgccag cacacaaaaa gttttgggaa
     4021 aaaaactttc ttgtaacgtg gggtcgcagg attatagaga agtcggggtc aaagatcgac
     4081 ccctttttgt gatttgtgac aggttttaac ccgccaaatg ctcgcgcttc agatactctt
     4141 cgctttgcat ctcttgcaga cgtgacaggc aacgctggaa ctcaaacttc agccgatcgc
     4201 cctgataaat ttcatacagc ggcacttctg cactcaccac taatttgaca tggcgctcgt
     4261 aaaactcatc caccagcgca ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa
     4321 ctggtacatc aaacaacatg accgtatgaa agaggcgtga gagcgcaata tagtcatgct
     4381 ggctgcgggc atcgacacac aaggtggtaa aagagacagc aagcgtctga ttctcagcgc
     4441 ccattgtcgc cagcggtcga tggttgattt ctaacgtcgg tgaattttcg cgtttcgctc
     4501 ccgccagcgc caaccatagt ttatccattt gtgcccgggt ttcatcgtgg agtggcgaaa
     4561 gccacagatg cgcctgagtg agtgtacgca gtcgataatc aacaccagcg tccacgttca
     4621 ttacatcaca atgctgttta atggcatcga ttgcaggcag aaaacgcgca cgttgcaagc
     4681 catttcgata aagttcgtcc ggcggaatat ttgacgtcgc taccagggta ataccgcggg
     4741 cgaacagggc tttcatcaga ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa
     4801 attcgtcaaa acagagcacg tcagtttcgg ctttaaagcg atcggcaata atttccagcg
     4861 gatcggtctg tccctgtaag gcagttagct cttcgtgcac ccgcagcata aaacggtgaa
     4921 agtgcaggcg ctgtttccgc tctcccggca ggctttgata gaaaaggtcc atcagccagg
     4981 ttttcccgcg tcctacaccg ccccacatat ataagccacg cactggcgta tgctttgtat
     5041 cttcgcgttt accccatagc ttaccgaccc gcgccattag cccactcgtc ctgggggctg
     5101 gtggcctgct attgatgagt tcttgataaa taatttccag gcggctgacg gcctcttttt
     5161 gaacgtcgtc gggttgatgg ctgccttcat tgagcgcctt caggtattgc gatgttgggg
     5221 taacgctttg catgatctta ttgttattcc ttgaataatc ggtgcgccgt tgttcacggt
     5281 tgacgaaaaa aaggccgttc tacactacgc gatatgcagt cgggattcca cttctgtgga
     5341 attaacggtt atagtggcat aatcagccgc aggcatggag cctgaagcca acaccctacg
     5401 gaaacaaaag acaacgggag atgttcatga cctgggaata tgcgctaatt gggttagtcg
     5461 tcggcatcat tattggtgct gtggccatgc gttttggtaa tcgtaaacta cgccagcaac
     5521 aggcgttgca gtacgaactg gaaaagaata aagctgaact ggacgagtat cgcgaagagc
     5581 tggttagcca ctttgcccgc agcgcggaat tactggatac catggcgcac gactatcgcc
     5641 agctgtatca gcacatggcg aaaagctcca gcagcctgct gccggaactg tctgctgaag
     5701 caaacccgtt ccgtaatcgt ctggcagagt ctgaagccag caacgatcag gcaccggtgc
     5761 agatgcctcg cgactattct gaaggcgcat ccggcctgct gcgtactggc gcgaagcgcg
     5821 actaatttat ttttcgggcg cagccattgc gccctcctct tctctccctc cccgactatc
     5881 atttaatgtg gtgtctcatt gttagccgtc tgaaaattca ataacatcaa actgttttga
     5941 atctcttttc ttatcattca ggtacgagag caggaataat gaaaaaacaa acccagctgt
     6001 tgagtgcatt agcgttaagt gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt
     6061 cgattccagg ccaggttgcc gatcaggccc ctctccccag tctggctcca atgctggaaa
     6121 aagtgcttcc ggcagtggtg agcgtacggg tggaaggaac ggccagtcag ggacagaaaa
     6181 tcccggaaga attcaaaaag ttttttggtg atgatttacc ggatcaacct gcacaaccct
     6241 tcgaaggttt aggctccggt gtcatcatca acgccagtaa aggctatgtg ctgaccaaca
     6301 accatgtgat taatcaggca cagaaaatca gtattcagct caatgatggg cgcgagtttg
     6361 atgcaaaact gattggtagc gatgaccaga gcgatatcgc cctgttacaa attcaaaacc
     6421 cgagcaaatt aacgcaaatc gctattgccg actccgataa attgcgcgtc ggtgattttg
     6481 ccgtagcggt cggtaaccca tttggccttg ggcaaaccgc cacctctggc attgtttccg
     6541 cattaggacg cagcgggttg aatcttgaag gtctggaaaa ctttatccag acagatgctt
     6601 ccattaaccg cggtaactcc ggcggtgcac tgttaaacct taacggtgag ttaattggca
     6661 tcaacactgc aatccttgcg cctggcggcg ggagcgtcgg gattggattt gccatcccca
     6721 gtaatatggc gcgaacactg gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt
     6781 tgttaggcat caaaggcacc gagatgagtg ccgatatcgc caaagccttc aaccttgacg
     6841 tgcagcgtgg cgcgtttgtc agcgaagtgt tgccaggttc tggctcggca aaagcgggcg
     6901 tcaaagcggg cgatattatt accagcctca acggcaaacc gctgaatagc tttgctgagt
     6961 tgcgctctcg tatcgcgacc accgagccgg gcacgaaagt gaagcttggc ctgctgcgta
     7021 acggcaaacc actagaagta gaagtgacgc tcgataccag cacctcttcg tcggccagcg
     7081 ctgaaatgat cacgccagcg ctggaaggtg caacgttgag cgatggtcag ctaaaagatg
     7141 gcggcaaagg tattaagatc gatgaagttg tcaaaggaag cccagctgct caggctggct
     7201 tgcaaaaaga cgatgtgatc attggcgtca accgcgatcg ggtgaactcg attgctgaaa
     7261 tgcgtaaagt gctggcggca aaaccggcca tcatcgccct gcaaattgta cgcggcaatg
     7321 aaagcatcta tctgctgatg cgttaatgtc gtaaaccggg catcaggctt acgtgtgatg
     7381 tccggttaac tcgtggtatg ctgctgccgt tccctttttt aatgacgcct ccatcatgtt
     7441 tgtgaagctc ttacgttccg ttgcgattgg attaattgtc ggcgctattc tgctggttgc
     7501 catgccttcg ctgcgcagcc ttaacccgct ttccactccg caatttgaca gtaccgatga
     7561 gacgcctgcc agctataatc tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta
     7621 caaccgtggt ttgaacacca actctcacaa ccagcttgag atccgcaccc tgggatccgg
     7681 tgtaatcatg gatcaacgcg gttatatcat caccaataaa cacgtcatca acgacgccga
     7741 tcagatcatc gtcgccttac aggatggacg tgtatttgaa gcattgctgg tgggatctga
     7801 ctctctaacc gatctggcgg tacttaaaat taatgccact ggcggtttac ctaccattcc
     7861 aattaatgca cgtcgcgtac cgcacattgg cgacgtagta ctggcgatcg gtaacccgta
     7921 caacctcggg cagaccatta cccaggggat tattagtgcc acgggtcgaa tcggtctgaa
     7981 cccgaccggg cggcaaaact tcctacaaac cgatgcttcc attaaccacg gtaactctgg
     8041 cggcgcgctg gtgaactcgc tgggagaact gatgggcatt aacacgctgt cgtttgataa
     8101 gagtaacgat ggcgaaacgc cggaaggtat cggctttgcg attcctttcc agttagcaac
     8161 caaaattatg gataagctga tccgcgatgg tcgcgtgatc cgcggctaca ttggtatcgg
     8221 cggacgtgag atcgcaccac tgcacgcgca gggcggtggt atagatcaac tgcaagggat
     8281 cgtggttaat gaagtgtcac ctgacggccc ggcggcgaat gcgggtattc aggtcaacga
     8341 tctgattatt tcggtggata acaaaccggc catctctgct ctggagacga tggatcaggt
     8401 ggcagaaatt cgccctggtt cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt
     8461 aacgctgcag gtcaccattc aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca
     8521 aaaaaccgga gtctgtgctc cggtttttta ttattcgcta attaattact tattaacgaa
     8581 ctcttcgccc agggcgatat ctttcttcag cgtatccaac ataccttcca gtgcgctctg
     8641 ttcaaatgcg ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag
     8701 cagcggttga gagaagaaac gtgcgtactg accgtcgcct tcaacatagg cacattcgac
     8761 aacgccttgt tcgccctgca gtgcgcgtac cagagacaga ccaaaacgtg cagctgcctg
     8821 gcccatagac agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacctgc
     8881 gttctggata cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac
     8941 ctgtgacagc agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac
     9001 ttcgcctggc tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatatccag
     9061 cgtggtaacg ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc
     9121 agcaattgca acagtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca
     9181 ggttttcgca acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc
     9241 ggaacgatcc atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc
     9301 ttccagcgcc ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat
     9361 atggctcaga tcgacggcca caccgggagt cactggagcg atatcataca gagagagttc
     9421 tgaacctgaa ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc
     9481 agcgccgagg actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg
     9541 ttgctccgct gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag
     9601 aatgtgtagt cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac
     9661 ttacgtaatt aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata
     9721 ccctaccgtt cagccaaaac aacatcaatt tgataacaat taatttactt ttaagcagaa
     9781 tttgcatgcc gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt
     9841 tcataacatt atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg
     9901 cacaataatg ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag
     9961 aagaactagt taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg
    10021 aaatcgtcgc cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc
    10081 ggatgttgac caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact
    10141 gcctgccagc tgaactgggt gtaccaacta cctccagtcc attgaagaat ctggtgctgg
    10201 atatcgacta caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa
    10261 ttgctcgcct gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg
    10321 atgacaccat ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga
    10381 ttttagagct gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa
    10441 aatgttgctt atccctctca ttccccttct ttctcctgcg atacatttaa cgaatagtgc
    10501 gttttaccgc gacacatcat tcacaaaatg aatacatacg gtaaaaaaag cacattatgc
    10561 aaaattcatt
//
LOCUS       DAZFAH010000157        10536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFAH010000157 DAZFAH010000000
VERSION     DAZFAH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.158"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            252..794
                     /gene="yfaZ"
                     /locus_tag="ACHJWB_003677"
     CDS             252..794
                     /gene="yfaZ"
                     /locus_tag="ACHJWB_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4315246.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            894..2096
                     /gene="yfaY"
                     /locus_tag="ACHJWB_003678"
     CDS             894..2096
                     /gene="yfaY"
                     /locus_tag="ACHJWB_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4315247.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2160..2621
                     /locus_tag="ACHJWB_003679"
                     /pseudo
     CDS             2160..2621
                     /locus_tag="ACHJWB_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2662..3564)
                     /gene="rpnE"
                     /locus_tag="ACHJWB_003680"
     CDS             complement(2662..3564)
                     /gene="rpnE"
                     /locus_tag="ACHJWB_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4315248.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3758..4948)
                     /gene="glpC"
                     /locus_tag="ACHJWB_003681"
     CDS             complement(3758..4948)
                     /gene="glpC"
                     /locus_tag="ACHJWB_003681"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4315249.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4945..6204)
                     /gene="glpB"
                     /locus_tag="ACHJWB_003682"
     CDS             complement(4945..6204)
                     /gene="glpB"
                     /locus_tag="ACHJWB_003682"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4315250.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6194..7822)
                     /gene="glpA"
                     /locus_tag="ACHJWB_003683"
     CDS             complement(6194..7822)
                     /gene="glpA"
                     /locus_tag="ACHJWB_003683"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4315251.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEKAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            8095..9453
                     /gene="glpT"
                     /locus_tag="ACHJWB_003684"
     CDS             8095..9453
                     /gene="glpT"
                     /locus_tag="ACHJWB_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4315252.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9458..10534
                     /gene="glpQ"
                     /locus_tag="ACHJWB_003685"
     CDS             9458..10534
                     /gene="glpQ"
                     /locus_tag="ACHJWB_003685"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4315253.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2408 a         2609 c         2912 g         2607 t
ORIGIN      
        1 accatgagaa tacggtaact cagaaatatg tgaaagtgac agtaatctct tttgcaatga
       61 taactgcact tattattatt tttgtgtgta tcggtaaagt gtgtttattt tttcggcgtt
      121 taattattta agaagtattt agttacaata agtgactaga tccctttttt cgctcattga
      181 atcctctgtg ccaggcagta caatcccgca gctttgggaa ttttgattaa ttgcttgtgg
      241 gaaaggggat aatgaaaaaa attgcgctag caggtctggc cggaatgctg ttggtttctg
      301 catcggtcaa tgcaatgagc atcagcggcc aggcgggtaa agaatacacc aatattggtg
      361 tcggttttgg gactgaatcg acgggcctgg ctttaagcgg taactggaca cataacgacg
      421 acgacggtga cgtcgcgggc gtggggctgg ggttgaatct gcctctcggg ccgttaatgg
      481 cgaccgttgg cggaaaaggc gtttacacca acccgaatta cggcgatgaa ggttatgccg
      541 cagcggtagg aggtggtttg cagtggaaaa ttggcaacag cttccgtttg tttggcgagt
      601 attactactc tccggattcg ctctccagcg gtattcaaag ttatgaggaa gcgaatgctg
      661 gcgcgcgtta caccattatg cgtccagtca gtattgaggc gggttatcgc tacctgaatc
      721 tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga cggcccgtat gttggggtta
      781 acgccagttt ctgatccctt ccggcgcggc attctgtcgc gccattcagt ttcttactac
      841 gccttatctc cctttgcgtt atagtgattc gataacaaaa gcaggagata accatgttaa
      901 aagtggaaat gctatccacc ggggatgaag tgttacacgg gcaaatcgtt gacactaacg
      961 ctgcctggct ggccgatttt ttctttcatc aggggctgcc attatctcgc cgcaatacgg
     1021 tgggggataa ccttgatgac ttagtcacca ttcttcgcga acgtagtcag cacgccgatg
     1081 tgctgatcgt taacggcggg ctgggaccga ccagcgatga tttaagcgca ctcgccgctg
     1141 cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg gctcaaagag atggaacgct
     1201 atttccacga acgtggacga gtaatggcac cgagcaaccg taaacaagcg gagctgcctg
     1261 ccagtgctga atttatcaat aacccggtag gcaccgcctg tggttttgcc gtgcagctta
     1321 atcgttgcct gatgttcttt actcccggcg taccgtcaga atttaaggtg atggtcgagc
     1381 acgaaatcct gccgcgtctg cgcgagcgtt tttctttacc acagccgccg gtttgtctgc
     1441 gtttaactac ctttggtcgt tcggaaagcg atctggcaca aagcctggac actctacaac
     1501 tgccgccggg cgtaacaatg ggctatcgct cttcaatgcc gattatcgaa ctgaaactca
     1561 ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt aaacgtgttg
     1621 ccggacagag cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc agtcgcgaat
     1681 tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt caccggtggt ttattggctt
     1741 tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga agtggttcct tcacaggaag
     1801 aaaccctggc gcaaactgcg cactggatta cagaacggcg ggccaaccat tttgccgggc
     1861 tggcactggc tgtttcgggt ttcgagaacg aacatctcaa ctttgcgcta gccacaccag
     1921 acggcacttt cgctctgcgt gtgcgtttca gcactacgcg ctacagcctg gctatccgtc
     1981 aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg ttggttaaac ggccaggata
     2041 tcgccagtga gcatggctgg attgaggtta ttgagtccat gaccttatct gtctgattcc
     2101 gccactctgt gccggtttac tgcaccggca cattccccct ccggcaaaac ttgtccaacc
     2161 ctccctacgg tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc tggggacgca
     2221 ttgagaatta catggcgcaa gtcaacgatt cgctttgtct gttagtgcag gtggaaagta
     2281 aaacggcact ggataacctg gacgagatcc tcgacgtcga agggattgat ggcgtgttta
     2341 ttggaccagc ggatctttct gcgtcgttgg gctacccgga taacgccggg cacccggaag
     2401 tgcagcgaat tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa gcggctggtt
     2461 ttctggctgt ggctcctgat atggcgcagc aatgcctggc gtggggagcg aactttgtcg
     2521 ctgttggcgt tgacacgatg ctctacagcg atgccctgga tcaacgactg gcgatgttta
     2581 aatcaggcaa aaatgggcca cgcataaaag gtagttattg atatcaaagg cccatgggga
     2641 tcggctgtgg gcctgtgtta attagtggtt attcgctgcc agatcggctt cgcttagctg
     2701 ggtggccgcg agcacctggt cacgatcaat gccctgttcc agcatgcgca aagcaatttt
     2761 aatggcttgt tcatgcatac cttcctgcca gccaatttga tgcccttcct gccgtagccg
     2821 ttcagcaata gtcattaatc tctccttttg tagtggtgaa cgttcggcaa tctcctcaat
     2881 aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag
     2941 ctggctgtca ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga ccatgccgat
     3001 taaatcgcgg tcgcgaatat gcttttgaat cagttccagc agagctatac gccgatgttg
     3061 catgatctca tcgtcaggta cgatggtgat atccaccaac ggaaaagctt cggtgtacaa
     3121 ctgccgggca agttgcggat cgtcaaactc atccagccag ttaagtgagt acgggtaggg
     3181 cgatgtttcg ccatgataaa acagcaatgg caccaccagc ggaactctgt catagccttt
     3241 atccaggtga cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt
     3301 ttctgcagag ctttgatgtt caatcacgca gtagatatag ccgtcaccgt cgctggtttc
     3361 cacggaccac aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactagtggg
     3421 ttccaggcgt aaggtttgca ggttgcaaag cttgcgcagt ggttctggta aatgtatttc
     3481 gagaaaatcc cgtgcggttt cgggtgtgaa cataaaggtt ttaaacaccg catcatgcgg
     3541 ggaggatgtt gttgattcgg tcatggttgt ccgtcacctg atatttatga tgacgcgaca
     3601 ttacgtctat cccgggcctt tgacatctgt cagtttcagc ctttgcgaga cattttcaga
     3661 ggaattttca aagcgttgca acgcatacat ggtgcgtgga atcgcgctcg caaaaatgcc
     3721 cgataagcaa tgtgcttacc gggcatcaaa aggtcggtta agccagcgcc tgggccagta
     3781 gcgtaatcgg atgttcgcag cgaaggctcg tggacatctc aatctgccat ttgcaggttt
     3841 cgcagtcggt gatgaccaga tccgcgccgc tctcttctat ctggcggaac agtggcgcac
     3901 cgatggcttg tgaggtgggg tagttctctt ttttgaaacc gtaagtaccc gcaataccgc
     3961 agcactggga atccagcacc gttaactcaa gccccgggat tttacgcaac agctccaggg
     4021 tgtagagcgt ccagcccatt ttttccatat ggcacggagt gtgataaacc actttcagcg
     4081 gcagcggttt cagcggtaac gttttgcctt cgtccagctt gcgccacagc cagcgggttg
     4141 ccagttcgat atgatcgcgc aagcctttgt tgtcgacatt cagcacttcc gggtattcgt
     4201 cgcgcagggc aaatgtacag gttgaggagg tggcaatcac cggaatgcct tttactccca
     4261 cagcttcgcg gatcgactct acattcgtaa ttgcctgttt gcgtgcttta tcggtaaagc
     4321 cgttggcgat tagcggtacg ccgcagcatt tttctttgct gagcagttgt acaccggtac
     4381 ccattgcgtt gagcacttta attaaatctt tacctaactg cggatggttg tagttaacga
     4441 agcagccgtg aaagaaagcg acctggtctt tatattgtgc ttgctgagcc gctatgctgc
     4501 gataccagcg acggaacgtg ccgaaggagt atttcggtag cgtgcggcga tgatcgattt
     4561 ttaacgccgc atcaagcagc tgccgcaccg gtttcagcga ggtagcggtg ttgacgattg
     4621 gtgcgaacgg cgtggaaacg ctacccatca ggtcggtatg actcaacaca aaattacgca
     4681 gcgacgggcg cgtggtgtca tattttgccc gcgcgcgctg gataatatcg ccaatcttca
     4741 catcggacgg gcaggcgact tcacaacgtt tgcagttgat gcaatatttc agcgcctcgt
     4801 catacagtgc gccatctttc aaacgcagac gctcgccatc cggcccggct tgttttggcc
     4861 ctggataacc gggattcacc cggctcaccg ggcaggcggt ggtgcagacg gtgcacttaa
     4921 tgcagttttc gaagctggtg tcgttcattg ttggcctcct gcgcgttggg caatctgttg
     4981 agcggcatgt aaagcactga cggcacaaac accgccgccg catccctggg cgatgggatc
     5041 aaatccgccc agcaccgaac cgatggcaaa caggttctca atggtttgcc ctgcctgcga
     5101 cgggcgtagc gtctcatcag tggttacacc gaactgctgc cacggttgcg gcgcaaaaaa
     5161 atcgccctta taccattcac cccgcgtggc ggtttgtagc acatcaaggc cgagaatcgg
     5221 ctcgcgaatg ccgttacgtt ccgctaccag tccgccacta aagaaactgc cgctggcgag
     5281 aaccgcgaaa cgtggacgta gcggaatatc ggcgtgattg cgggtccaga tttcattcac
     5341 tacgccattt ttacaggtca cttttttcac ttcatcgccc ggcatccaca cgccaccctg
     5401 gcgcacaaac tggcgctgta actggttttg cagacgaatg cccagcacgg aaggcggcag
     5461 cgttggcaaa agcatcagtg aacaaggtag tttttcattc aaccaacgcc acagtttgtc
     5521 atcggccagg ccgaagcagg cgggcatcag gatcatttcg caggtattgg cgacaggaat
     5581 aagcgcatca agtaacagcg gccagttttc ttcattatca aggaaacggg cgatattcac
     5641 cgcgcgaaat tcggtggcgt tattgcgcag gacatccagt tccggcagct ctatttctgc
     5701 ggtttcaacc gaaagatcca gttcgcgcaa cgaagccgcc gcaagatgcg cctggaaatc
     5761 catcagcccg cttatcccca ctacacatat cttcttcgcg ggtagcggcc agacggggac
     5821 ttctggcgaa cttagccagg tagagcgcag agtgccgagc ggcgtaatcc gctggtgagc
     5881 cagttctacg ctgccctgca attgcgcacc gctttcagcg attaatgcct gcgcctggca
     5941 agcgagatcg agcacgcgtt gtggcccgag aagagagtaa ggatgtgcag gtgcctgctg
     6001 acgcaaagat tccagtccac tgtgaatgtc tgccacgggt tgaccatctg gcagatggct
     6061 cagcaaatcc agcgaaccgg atgagaaatg caacgcgctt tgaccacgcg tgacaatggc
     6121 acagcgcagg ccgtgttttt gcagttgcag gccacagagt aatccggcga ggccgccgcc
     6181 cataatgaca gtatcaaagc gcatctttct gctccttctc cagaccacac agtccctgat
     6241 aaacccagcg ggtaaattcg ctttcgcgca gtgcgtctcc ccaggcgatg ggttgcacgc
     6301 ctttccagcg ttcgttaagg aaggtggaaa gttgctcgat agattgcgcg gacgtcgtga
     6361 cgttaaaccg ttgcagcagt ccggcagcgc ggcaggcgca gagttcgccc tggcaggtgc
     6421 ccatccccac acgggtacga cggcgtaaat ccagcaggct attaacgttt aaattttcta
     6481 ccgcgtactg cacttcaccc gcagttaccg cttcgcactc acataccagg ctacggtgca
     6541 gacggccttc gctcagccag gcaggcgtgc gatcgccatg acgataaacc gcagaaccgc
     6601 gcagcggggc aggcagggag atgactttac gcaaggtaac ttcagcgggt tcttgtgaac
     6661 caggcagtgc cagatcggca gtcgtacagg ggcgcgtgtt gcccagtttg cggcataccg
     6721 cgtcggtagc ccattcagcc atcagccgat aggtcatcag tttgccaccg gtgatggtga
     6781 taaatccgtc cagaccatcg cgttcagcat ggtcgagcag cacgatgcca cggctgacgt
     6841 tacgtccgct cgggtcgtca tcgctggcaa ccagcgggcg cacgccagaa taggcccgca
     6901 aaatgcgcgt tttcgccatc acgggggcca gtttttcccc ttcacgcagc agaatatcaa
     6961 cctcttctgc cgtcactcga ttatcgtcaa tctcgttgta atcaatacgt aaagaggtgg
     7021 tgccaatcag cgaaatggta tcgccaggca ccagaatatc ggcgtcggaa ggtttacggc
     7081 agcggttgat cacatgctgg ttaatgcggt gatccatgat cagcagcgat cctttcgccg
     7141 ggaacatgcg aatgcgcaga tcggcatatt cggcaatgtg ttgcccccag atcccagcgg
     7201 cattaaccac gacaggtgca tgaagggcct gagtttcgcc ggtgagatgg ttacgtacac
     7261 gaacaccgca caccgtcgcg ccttcacgaa tcagccccgt gacttcatga gcggtaagga
     7321 taacggcacc gtgttctttg gcatccagca tgtttgctgc ggtcagacga aatggatcaa
     7381 cggtgccatc cggaactttc accgcgccaa tcagtgccgg gttaacggca ggttcgataa
     7441 tgcgcgcttg ctgcgggtct atagcttctg cgctgatccc tgctttttcg caggcgcgaa
     7501 taaaagtggc ctggaaggag aggtcatctt ccggcagggt gataaacagg ccgttggttg
     7561 gttcaacgca gtgacgtgca atgcgtttca ggatctggtt ttcactaatg cattcgcggg
     7621 ccgattccgc atcggttacc gcatagcgcg caccgctgtg cagcaggccg tggttacgcc
     7681 cggtggcacc ggttgcgatg tcgtggcgct caaccaaaat cacgcgcagc ccgcgcaggg
     7741 cacagtcgcg ggcaatcccg gctcccgttg cgccgccgcc aatgataatc acgtcacttg
     7801 attgcgagtc gcgagttttc attgttatcc ctctgaagtt cgttttttac catttagcca
     7861 tagtaaaaac atgaattgtt tgatttcgcg catattcgct cataattcga aagtgaaacg
     7921 tgatttcatg cgtcattttg aacattttgt aaatcttatt taataatgtg tgcggcaatt
     7981 cacatttaat ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg gcaggttctc
     8041 tcactgaatc aggctgttaa tcataaataa gaccacgggc cacggaggct atcaatgttg
     8101 agtattttta aaccagcgcc acacaaagcg cgcttacctg ccgcggagat cgatccgact
     8161 tatcgtcgat tgcgctggca aattttcctg gggatattct ttggctatgc ggcttactat
     8221 ttggttcgta agaactttgc gcttgctatg ccttatctgg ttgagcaggg attctcacgc
     8281 ggtgatttag gttttgccct ttcggggatc tcgattgctt atggattttc gaaattcatc
     8341 atgggttcgg tatcggatcg ctcgaatccg cgcgttttcc tgcccgcagg tttgattctg
     8401 gcggcggcag tgatgttgtt tatgggcttt gtgccatggg cgacgtcgag cattgcggtg
     8461 atgtttgtac tgttgttcct ctgcggttgg ttccagggga tggggtggcc gccgtgtggt
     8521 cgtactatgg tgcactggtg gtcgcagaaa gaacgtggcg gcattgtgtc agtgtggaac
     8581 tgtgcgcaca acgtcggtgg tggtattccg ccgctgctgt tcctgctggg gatggcctgg
     8641 ttcaatgact ggcatgcggc gctctatatg cctgctttct gcgccattct ggtggcatta
     8701 ttcgcctttg cgatgatgcg cgataccccg caatcctgtg gcttgccgcc gatcgaagag
     8761 tacaaaaatg attatccgga cgactataac gaaaaagcgg aacaggagct gaccgcgaag
     8821 caaatcttca tgcagtacgt actgccgaac aaactgctgt ggtatatcgc catcgccaac
     8881 gtgttcgttt atctgctgcg ttacggcatc ctcgactggt caccgactta tctgaaagag
     8941 gttaagcatt tcgcgctgga taaatcctcc tgggcctact tcctttatga atatgcaggt
     9001 attccgggca ctctgctgtg cggctggatg tcggataaag tcttccgtgg caaccgtggg
     9061 gcaaccggcg ttttctttat gacactggtg accatcgcga ctatcgttta ctggatgaac
     9121 ccggcaggta acccaaccgt cgatatgatt tgtatgattg ttatcggctt cctgatctac
     9181 ggtcctgtga tgctgatcgg tctgcatgcg ctggaactgg caccgaaaaa agcggcaggt
     9241 acggcagcgg gctttaccgg gctgtttggt tacctgggcg gttcggtggc ggcgagcgcg
     9301 attgttggct acaccgtgga cttcttcggc tgggatggcg gctttatggt aatgattggc
     9361 ggcagcattc tggcggttat cttgttgatt gttgtgatga ttggcgaaaa acgtcgccat
     9421 gaacaattac tgcaaaaacg caacggaggc taatggcatg aaattgacgc tgaaaaacct
     9481 tagcatggcg atcatgatga gcactatagt catgggaagc agtgcaatgg cggcggacag
     9541 caacgaaaaa atagtcatcg cccatcgcgg tgccagtgga tatttgccgg agcatacgct
     9601 gccagcaaaa gcgatggcgt atgcgcaggg agcggattat ctggaacagg atttggtgat
     9661 gaccaaagac gaccatctgg ttgttctgca tgaccattac ctcgatcgtg ttactgatgt
     9721 tgccgatcgt ttcccggatc gggcgcgcaa agacggtcgt tactacgcga tagatttcac
     9781 gctggatgaa attaagtcgt tgaaatttac cgaaggtttc gatattgaaa acggtaaaaa
     9841 agtgcagact tatccggggc gtttcccaat gggtaagtcc gacttccggg tgcacacctt
     9901 tgaagaagag attgaatttg ttcaggggtt aaatcactct accgggaaaa atatcggtat
     9961 ttatccagaa atcaaagcgc cgtggttcca tcatcaggaa gggaaggata ttgcggcaaa
    10021 aacgctggaa gtgctgaaga aatatggtta caccggtaaa gacgataaag tttatttgca
    10081 atgttttgat gctgatgagc tgaagcgtat taagaatgag ctggaaccca aaatgggcat
    10141 ggagctcaat ctggtacagc tgattgccta taccgactgg aatgaaacgc agcagaaaca
    10201 gccggatgga agctgggtta attacaacta cgactggatg tttaagccgg gtgccatgaa
    10261 acaggtggcg gaatatgcag atggtattgg tccggattac catatgttga ttgaggagac
    10321 atcgcagccg ggtaatatca aactcactgg catggtgcaa gatgctcagc agaataaact
    10381 ggtagtgcat ccttataccg tgcggtcaga taaactgcct gaatacactc ctgatgtgaa
    10441 tcagttatat gatgctctgt ataacaaagc gggtgtaaat gggctgttta ctgatttccc
    10501 tgataaggca gtaaaatttc ttaataaaga gtaatg
//
LOCUS       DAZFAH010000158        10464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFAH010000158 DAZFAH010000000
VERSION     DAZFAH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.159"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..719
                     /gene="ssuE"
                     /locus_tag="ACHJWB_003686"
     CDS             144..719
                     /gene="ssuE"
                     /locus_tag="ACHJWB_003686"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4315254.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            712..1671
                     /gene="ssuA"
                     /locus_tag="ACHJWB_003687"
     CDS             712..1671
                     /gene="ssuA"
                     /locus_tag="ACHJWB_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4315255.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1668..2813
                     /gene="ssuD"
                     /locus_tag="ACHJWB_003688"
     CDS             1668..2813
                     /gene="ssuD"
                     /locus_tag="ACHJWB_003688"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4315256.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPHKVAQS"
     gene            2824..3615
                     /gene="ssuC"
                     /locus_tag="ACHJWB_003689"
     CDS             2824..3615
                     /gene="ssuC"
                     /locus_tag="ACHJWB_003689"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO4315257.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3612..4379
                     /gene="ssuB"
                     /locus_tag="ACHJWB_003690"
     CDS             3612..4379
                     /gene="ssuB"
                     /locus_tag="ACHJWB_003690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4315258.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4586..7198)
                     /gene="pepN"
                     /locus_tag="ACHJWB_003691"
     CDS             complement(4586..7198)
                     /gene="pepN"
                     /locus_tag="ACHJWB_003691"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4315259.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRSDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7464..8666
                     /gene="pncB"
                     /locus_tag="ACHJWB_003692"
     CDS             7464..8666
                     /gene="pncB"
                     /locus_tag="ACHJWB_003692"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4315260.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8835..10235
                     /gene="asnS"
                     /locus_tag="ACHJWB_003693"
     CDS             8835..10235
                     /gene="asnS"
                     /locus_tag="ACHJWB_003693"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4315261.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLEKLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2369 a         2702 c         2811 g         2582 t
ORIGIN      
        1 gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaaaga
      121 acaattggtt ataaggagag agtatgcgtg tcatcaccct ggcgggtagt cctcgctttc
      181 cttctcgctc cagctccttg ctggaatatg cgcgggaaaa actaaatggc ctcgatgtag
      241 aggtttatca ctggaatctg caaaacttcg ccccggaaga tctgctttat gctcgtttcg
      301 atagtccggc actcaagacc ttcaccgaac agctgcaaca ggctgatgga ctgatagtcg
      361 ctacacctgt gtataaagcc gcctattccg gcgcgctgaa aaccctgctc gacctgctgc
      421 cagagcgcgc tttgcaaggc aaagtggtgc taccgctggc gacgggcggt accgtggccc
      481 atctgctggc ggtcgattat gcccttaaac cagttttaag cgcactgaaa gctcaggaga
      541 tcctgcacgg cgtgtttgcc gatgactcac aagtaattga ttaccatcac agaccccagt
      601 tcacgccaaa tctgcaaacc cgtcttgata ccgcgctaga aactttctgg caggcattgc
      661 accgccgcga tgttcaggtt cctgaccttc tgtctctgcg aggtaatgcc catgcgtaac
      721 atcattaaac tggcgctggt gggattgctt agcgtctcta cgattgcggt tgctgcagaa
      781 tcctcgcctg aagcgttacg tataggctat cagaaaggca gtattggtat ggtactggca
      841 aaaagccacc agttactgga aaaacgctat ccgcaatcaa aaatctcctg ggtggagttc
      901 cccgcgggtc cgcaaatgtt ggaagcgtta aacgttggca gtattgatct cggcagtacc
      961 ggggatattc cgccaatctt tgcccaggct gccggggctg atttggtata tgtgggcgtc
     1021 gagccaccga agcccaaagc cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc
     1081 gtagccgatc ttaaaggtca caaagttgcc tttcagaaag gttccagttc acacaacctt
     1141 ttactgcgtg cactgcgtca ggccggactt aagtttaccg atatccaacc cacttacctg
     1201 acgcccgctg atgcccgcgc cgcgttccag caaggtaacg ttgacgcctg ggctatctgg
     1261 gatccctact actctgctgc attattacag ggcggcgtgc gggtgctgaa agacggcacc
     1321 gatctcaatc aaactggatc gttttatctg gcagctcgtc cctatgcaga aaaaaacggc
     1381 gcttttattc agggcgtact cgcaaccttt agtgaggccg atgcgttaac ccgcagccag
     1441 cgcgagcaaa gcatcgcttt actggcaaaa acgatgggct taccggcacc ggtgattgcc
     1501 tcttacttag atcatcgccc tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc
     1561 ttacagcagc aaacggcaga tctgttttat gaaaatcgtc tggtgccgaa aaaagtcgat
     1621 attcgccagc gcatctggca gcccactcaa ctggaaggaa aacaattatg agtctgaata
     1681 tgttctggtt tttaccgacc catggtgacg ggcattatct tggaacggaa gaaggttcac
     1741 gcccggttga tcacggctat ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggctata
     1801 ccggtgtgct gatcccaacg gggcgatcgt gtgaagatgc gtggctggtg gcggcgtcga
     1861 tgatcccggt gacacagcgg ctgaagtttc ttgtcgccct gcgccccagc gtaacctcac
     1921 ctaccgttgc cgctcgccag gccgccacgc ttgaccgtct ctcaaatggt cgtgcgttgt
     1981 ttaatctggt cacaggcagc gatccacaag agctggcagg cgacggcgtg ttccttgatc
     2041 atagcgagcg ctacgaagcc tcggcggaat ttacccaggt ctggcggcgc ttattgctgg
     2101 gggaaaccgt caatttcaac ggtaaacata ttcatgtgcg cggagcaaaa ctgctcttcc
     2161 cgccgattca acagccttat cctccgcttt actttggcgg atcgtcagac gtcgcccagg
     2221 agctggcggc agaacaagtt gatctctacc tcacctgggg cgaaccgcca gaactagtaa
     2281 aagagaagat cgaacaagtg cgggcgaaag ctgccgcgca tggacgcaaa attcgtttcg
     2341 gtattcgtct gcatgtgatt gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc
     2401 ggttaatctc gcatcttgat gatgaaacta tcgccaaagc acaggccgca ttcgcccgga
     2461 cggattccgt aggacaacag cgaatggcgg cgttacataa cggcaagcgc gacaatctgg
     2521 agatcagccc caatttatgg gcgggcgttg gcttagtgcg cggcggtgcc ggaacggcgc
     2581 tggtgggcga tggtcctacg gtcgctgcgc gaatcaacga atacgccgcg cttggcatcg
     2641 acagctttgt gctttcgggc tatccgcatc tggaagaagc gtatcgggtc ggcgagttgc
     2701 tgttcccaca tctggatgtc gccattccgg aaattcccca gccgcagccg ctgaatccgc
     2761 aaggcgaagc ggtggcgaat gattttatcc cccataaagt cgcgcaaagc taaggaagaa
     2821 ataatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg
     2881 ggcatcgtgg cggtgtggca actggcctcc tcagttggct ggctgtcgac gcgtattttg
     2941 ccctcaccgg aaggggtggt gacggcgttc tggacgctct ccgccagcgg cgaactgtgg
     3001 cagcatctgg cgatcagttc ctggcgggcg ctgattggct tttcaattgg cggatcgctg
     3061 gggttgattt tgggactgat tagcgggcta tctcgctggg gagaacggct gctggatacc
     3121 tcaattcaga tgttgcgcaa cgtaccgcat ctggcgctga tcccgctggt gattttgtgg
     3181 tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggcacgct attccccatt
     3241 tatatcaaca cctggcatgg catccgtaat atcgatcgcg gtctggtgga aatggcgcgt
     3301 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc
     3361 tcaattatgg tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc
     3421 gaaaccattt ctgccaattc aggcattggt tatctggcga tgaatgcgcg ggagtttttg
     3481 caaacggacg tggtggtagt cgctattatt ctttacgccc tgctcggcaa acttgctgac
     3541 gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag
     3601 gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag
     3661 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     3721 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     3781 tggccgggct ggaaacgcca accgcaggcg atgtgttggc aggcaccaca ccgctggctg
     3841 aaattcagga agacacgcga atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg
     3901 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt
     3961 tggctgcggt aggactggag aatcgcgccg gggaatggcc tgccgcactt tcaggcgggc
     4021 agaaacagcg agtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg
     4081 atgaaccgct cggggcactg gacgccttaa cgcgactcga gatgcaggat ttgattgtgt
     4141 cactttggca gcagcacggc tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     4201 tagcaatggc tgaccgggtg ttgttaattg aagagggaaa aattggtctg gatttgacag
     4261 tggatatccc ccgtccgcgt cgattagggt cggtgcgatt agcggagctg gaagcggaag
     4321 tattgcaacg ggtaatgcgg cgtgggcatt ctgaacaact gattcggcgt catggttaat
     4381 atgccggatg tggcgcacaa aaccagcatt ttcaacggac ttgtaggcct gataagacac
     4441 gtcaagcatc gcatcaggca gttgggcacg gctgccggat gcggcgtgaa cgccttatcc
     4501 ggcctacaaa accagcaatt tcaacggagt tgtaggcctg ataagacgcg tcaagcgtcg
     4561 catcaggcat tttggctcaa aagtatcaag ccagtgcttt agttatcttc tcgtaaagat
     4621 cgccagagag attttccagc cctttcaact gttccagcgc cgcgcgcatt ttctcctgac
     4681 gtttggcatc ataacgtttc aggcgaatca gcggttcaat cagacgtgaa gctacctgcg
     4741 ggttacggct gttgaggtcg gtaagcattt ccaccaggaa ctggtaaccg ctgccatctt
     4801 cggcatggaa cgctgccggg ttgctgcctg caaacgcacc aatcaacgaa cgaatacggt
     4861 tcgggttgct catggtaaat gagcgatgct gcaacagacc acgcaccgtc tccagcacat
     4921 tcgccgccgg gctggtggct tgcaggataa accatttatc catcaccaga ccgtcctgat
     4981 gccacttatc gtcgtactcc tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg
     5041 ccgcagaaag cgctgccagc gcatcggtca tattgttcgc ttcgtggaac tgcttgctca
     5101 ccagcacgtc agccagatgc gtttcaccaa aagcaaggaa gcgcaggcag gcattacgca
     5161 gagtgcgttt cgcaatatct tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt
     5221 aaatagccag cagctcatcc gccagttcag tcgccagagt acgagtgagt gcttcgcgta
     5281 cttcggcaat agcaatcggg tcgatgatat cgaacagttc agccatttca ttgacagaag
     5341 gcagcgtcag gatttctgcc gccagcgccg gatcaatctt ctcatcgagc agcactgcgc
     5401 ggaaagcgtc agccacatgc accggcagag acagcggctg cccttgctga tgtcgggcga
     5461 cgttcagctt gatgtaggtt gccagcaggc tttgtgccgc atcccagcgg gagaaatcat
     5521 tacgcgcatg acgcatcagg aaggtcagtt gctgatcgct ccacttatat tccagtttca
     5581 ctggcgcaga gaattcgcac agcagcgcag gcaccggctg gaagtagaca ttatcaaaaa
     5641 caaaggtctg ttccgcctgg gtgacgttca gcacggaatt caccggatga ccgcctttct
     5701 gcaacgagat cactttgcct tcgttgtcat acaattcgat ggcaaacgga atatgcagcg
     5761 gctgtttttc tgcctgatcc ggcgtggctg gcgtgcgctg gctgatggtc agggtgtact
     5821 gctcggtttc cggattgtag tcgtctttga cggtcacaat cggcgtgccg gactggctgt
     5881 accaacggcg gaaatgggag agatcgacat tcgacgcatc ttccatcgcc tgcacaaagt
     5941 cgtcgcaggt tgccgcgctg ccatcatggc gctcgaaata gagttgcatc cctttctgga
     6001 agttttcttc gcccagcagg gtgtggatca tacggatcac ttccgcgccc ttctcgtaaa
     6061 cggtcagggt gtagaagttg ttcatctcaa tgaccatgtc cgggcggatc gggtgcgcca
     6121 tcgggctggc gtcttccgca aactgcaatc cgcgcatggt gcgcacatta ttgatgcggt
     6181 taactgcacg ggaaccaagg tcagagctga attcctgatc gcggaagacg gttaaacctt
     6241 ctttcaggct gagctggaac cagtcgcggc aggtgactcg gttaccggtc cagttatgga
     6301 aatattcatg gccgataacg cgttcaatat cgaggtaatc tttgtcagtg gcggtgtcgg
     6361 tgcgggccag cacatatttg gagttaaaga tattcaaacc cttattctcc attgcgccca
     6421 tattgaagaa atccaccgcg acgatcatat agatgtcgag gtcatactcc aggccgaagc
     6481 gttcttcatc ccatttcatt gagtttttca gcgaggtcat cgcccacggc gcgcgatcaa
     6541 ggttgccgcg atcgacgtac agctccagcg ccacttcgcg accagaacgc gtggtaaagg
     6601 tgtcgcgcag tacatcaaag tcgcctgcca ccagcgcaaa caggtagcac ggtttcggga
     6661 acgggtcctg ccactgtacc caatggcgtc cgttttccag ttcaccttgc gcaacgcggt
     6721 taccgttgga aagcaggaag ggatatttgg ttttatcggc aataattttg gtggtaaaac
     6781 gcgccagcac gtccgagcgg tcgagataat acgtaatatg gcggaaacct tcggcttcac
     6841 actgggtgca aagcgcatcg cctgactgat aaagcccttc cagcgccgta ttcgccgccg
     6901 ggcttatttc attaatgatc ttaagcgtaa aacgctccgg caaattactg atgaccagtg
     6961 cgccctcttc ttctttccag gcggtccacg gctcatcatt aatatgaaca gaaaccagtt
     7021 tgaggtcttc gccgttgaga cgaagcggag catctgatgc accatgacgg acagcctggc
     7081 tgaccgcggt aacgaccgtc ttttgcgcgt cgaggtcaaa ggtcaagtca atatcagtaa
     7141 tctggtaatc cggcgcacga taatcgtgac ggtatttggc ttgtggctgt tgagtcataa
     7201 ataaccttta gcatctttta tagagtctgg tgttcagtct attcctgttg cgtaaatcgc
     7261 gctatgcaga atcttcatct tttcaggtac aaacgccttt attgctacat ttttataaca
     7321 tacaccgcgt aatgccatcg accagaaagg tggcatatgg tgtgatcggg gttcaataaa
     7381 ttgcgaaaca aggtatactc cagcagttcc tgaagatgtt tattgtacta aacgctcctg
     7441 tacgaggacg ctactgcgca cctatgacac aattcgcttc tcctgttctg cactcgttgc
     7501 tggatacaga tgcttataag ttgcatatgc agcaagccgt gtttcatcac tattacgatg
     7561 tgcatgtcgc ggcggagttt cgttgccgag gtgacgatct gctgggtatt tatgccgatg
     7621 ctattcgtga acagattcag gcgatgcagc acctgcgcct gcaggatgat gaatatcagt
     7681 ggctctctgc cctgcctttc tttaaggccg actatcttaa ctggttacgc gagttccgct
     7741 ttaacccgga acaagtcacc gtgtccaacg ataatggcaa gctggatatt cgcttaagcg
     7801 gtccgtggcg tgaagtcatc ctctgggaag ttcctttgct ggcggttatc agtgaaatgg
     7861 tacatcgcta tcgctcaccg caggccgacg ttgcgcaagc cctcgacacg ctggaaagca
     7921 aattagtcga cttctcggcg ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg
     7981 attttggcac ccgtcgccgt ttttctcgcg aagtacaaga aaccatcgtt aagcgtctgc
     8041 aacaggaatc ctggttcgtg ggcaccagca actacgatct ggcgcgtcgg ctttccctca
     8101 cgccgatggg aacacaggca cacgaatggt tccaggcaca tcagcaaatc agcccggatc
     8161 tagccaacag ccagcgagct gcacttgctg cctggctgga agagtatccc gaccaacttg
     8221 gcattgcatt aaccgactgc atcactatgg atgctttcct gcgtgatttc ggtgtcgagt
     8281 tcgctagtcg gtatcagggc ctgcgtcatg actctggcga cccggttgaa tggggtgaaa
     8341 aagccattgc acattatgaa aagctgggaa ttgatccaca gagtaaaacg ctggttttct
     8401 ctgacaatct ggatttacgc aaagcggttg agctataccg ccacttctct tcccgcgtgc
     8461 aattaagttt tggtattggg actcgcctga cctgcgatat cccccaggta aaacccctga
     8521 atattgtgat taagttggta gagtgtaacg gtaaaccggt ggcgaaactt tctgacagcc
     8581 ctggcaaaac tatctgccac gataaagcgt ttgttcgggc gctgcgcaaa gcgttcgacc
     8641 ttccgcatat taaaaaagcc agttaatatc atcagggagc taatcggctc ccttttttta
     8701 cctttaattc cgaaatcttt cgctgcattt gcgaattctg cttgtctgat tgcagatgcc
     8761 aggtaacata ggtatccccc catttacggg tggacaagtg tttatttttt ccgactatta
     8821 acagagagaa tattatgagc gttgtgcctg tagccgacgt actccagggc cgcgtagccg
     8881 ttgacagcga agtcaccgta cgcggatggg tacgtacccg ccgagattca aaagctggca
     8941 tctccttcct cgccgtttat gacggttcct gctttgatcc tgtacaggct gtcatcaata
     9001 attctctgcc caattacaat gaagacgtcc tgcgtctgac caccggctgc tcggtcattg
     9061 tgacgggtaa agtcgtggcg tcgccgggcc aggggcaaca atttgaaatt caggccagca
     9121 aggttgaagt tgctggttgg gttgaagatc cagacactta cccgatggcg gcaaaacgcc
     9181 acagcattga gtatctgcgt gaagtcgctc acctgcgtcc gcgcacaaac ctgattggtg
     9241 ccgtcgcgcg cgttcgtcat acgctggcgc aggcgctgca tcgcttcttt aacgagcagg
     9301 gattcttctg ggtttcaacg ccactgatta ccgcatctga taccgaaggt gcaggcgaaa
     9361 tgttccgcgt ttctacgctg gatctggaaa aactgccgcg taacgatcag ggcaaagtgg
     9421 atttcgacaa agacttcttt ggtaaagagt ctttcctgac cgtatctggc cagttgaacg
     9481 gcgaaaccta cgcttgcgca ttatccaaaa tttatacctt cggcccgact ttccgtgctg
     9541 aaaactccaa caccagccgt cacctggcgg aattctggat gctggagccg gaagtggcgt
     9601 ttgctaacct gaacgatatc gcgggtctgg ctgaagccat gctgaaatat gtcttcaaag
     9661 cggttctcga agaacgcgca gacgacatga aattcttcgc tgaacgcgta gataaagatg
     9721 ccgtttcacg tctggaacgc ttcattgagg ccgatttcgc gcaggtggat tacaccgacg
     9781 cagtgaccat tctcgaaaac tgcggcagga agtttgaaaa cccggtttac tggggcgtcg
     9841 atctctcttc tgagcatgag cgttatctgg cggaagaaca ctttaaagca ccggtagtgg
     9901 ttaaaaacta tccgaaagat attaaagcgt tctatatgcg cctcaacgaa gacggtaaaa
     9961 ccgttgcggc tatggacgtt ctggctccgg gcatcggtga gatcattggt ggctcccagc
    10021 gtgaagaacg tctggacgtt ctggacgagc gtatgctgga aatgggcctg aataaagaag
    10081 attactggtg gtatcgcgat ctgcgtcgct acggtactgt tccgcattca ggtttcggtc
    10141 ttggttttga acgcctgatt gcttacgtaa ctggcgtgca aaacgtgcgt gatgtgattc
    10201 cgttcccacg tactccgcgc aacgccagct tctgattttc ttcctcaaag agccagcgcc
    10261 tgctggctct tttttatcct ttctgtcaag ttatctgttt gttaagtcaa gcaatctatt
    10321 tgcaaccccg ccataacgat catcctgtta cggaatatta cattgcaaca tttacgcaca
    10381 aaaaataacc cgcactctta ttgcggatta gttttttctt agctgatagc acaattttca
    10441 tactattttt tggcattctg gatg
//
LOCUS       DAZFAH010000159        10399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFAH010000159 DAZFAH010000000
VERSION     DAZFAH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.160"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..300
                     /locus_tag="ACHJWB_003694"
     CDS             <1..300
                     /locus_tag="ACHJWB_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4315262.1"
                     /translation="RWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTY
                     RGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            305..1048
                     /locus_tag="ACHJWB_003695"
     CDS             305..1048
                     /locus_tag="ACHJWB_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4315263.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            1045..1617
                     /locus_tag="ACHJWB_003696"
     CDS             1045..1617
                     /locus_tag="ACHJWB_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4315264.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1678..5157
                     /locus_tag="ACHJWB_003697"
     CDS             1678..5157
                     /locus_tag="ACHJWB_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4315265.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDVISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAVPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFCGAKHERENNDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            5225..5824
                     /locus_tag="ACHJWB_003698"
     CDS             5225..5824
                     /locus_tag="ACHJWB_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4315266.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5889..9242
                     /locus_tag="ACHJWB_003699"
     CDS             5889..9242
                     /locus_tag="ACHJWB_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4315267.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSASEAATRATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGCFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFLEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASA
                     VSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            9242..9826
                     /locus_tag="ACHJWB_003700"
     CDS             9242..9826
                     /locus_tag="ACHJWB_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4315268.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDEGSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            9799..9936
                     /locus_tag="ACHJWB_003701"
     CDS             9799..9936
                     /locus_tag="ACHJWB_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO4315269.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(9881..>10399)
                     /locus_tag="ACHJWB_003702"
     CDS             complement(9881..>10399)
                     /locus_tag="ACHJWB_003702"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4315270.1"
                     /translation="EHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRA
                     GESKIKHKISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDD
                     GTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQ
                     GTVVMFSASGKK"
BASE COUNT         2526 a         2584 c         3055 g         2234 t
ORIGIN      
        1 cgctggcgca tcgagcagtg cagcgaactg agcgcggtga gtgcctcctt tgtactgtcc
       61 acgccgacgg aaacagatgg cgctgttttt ccgggacgta tcatgctggc caacacctgc
      121 acctggacct atcgcggcga tgagtgcggt tatcacggtc cggcggtcgc ggatgaatat
      181 gaccagccaa cgtccgatat cacgaaggat aaatgcagca aatgcctgag cggttgtaag
      241 ttccgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa
      301 tcccatgaca gagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga
      361 gtcgtgcggc ttcgtggtaa gcacgccgga gggggaaaga tatttcccct gcgtgaatat
      421 ttccggtgag ccggaggatt atttccggat ggctccggag gactggctgc aggcagagat
      481 gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga
      541 ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc
      601 gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac
      661 ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt
      721 tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata atctggaggc
      781 cacagggctg tatcaggtgc cgttgtcagc ggcacagccg ggcgatgttc tgctgtgctg
      841 ttttggttca tcggtgccga atcacgccgc aatttactgc ggcgacggcg agctgctgca
      901 ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac
      961 acactccctc tggcgtcacc gggcatggca cgcatctgcc tttacgggga tttgcaacga
     1021 tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg cgcactggcc
     1081 acacagctcc cggcgtttcg tcagaaactg agcgacggct ggtatcaggt acggattgcc
     1141 gggcgggacg tcagcacgtc cgggttaacg gcgcagttac atgagactct gcctgacggc
     1201 gctgtgattc atattgttcc cagagtcgcc ggggccaagt cagggggcgt attccagatt
     1261 gttctggggg ctgccgccat tgccggatca ttctttactg ccggagccac tcttgcagca
     1321 tggggggcag ccattggggc cggtggtatg accggtatcc tgttttctct cggtgccagt
     1381 atggtacttg gtggtgtggc gcagatgctg gcaccgaaag ccaggacgcc cacggcagca
     1441 agtacagata acggcaaaca gaacacctat ttctcctcac tggataacat ggttgcccag
     1501 ggcaatgttc tgcccgttct gtacggtgaa atgcgcgtgg ggtcgcgggt ggtctctcag
     1561 gagatcagca cggcagacga aggggatggt ggtcaggttg tggtgattgg tcgctgatgc
     1621 aaaacatttt atgtgaaacc gcctgcgggc ggttttgtcg tttatggagc gtgacgaatg
     1681 ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct gaagtccacg
     1741 cagctgctga gtgtgatcga tgtcatcagc gaagggccga ttgaaggtcc ggtggatgga
     1801 ttaaaaagcg tgctgctgaa cagtacgccg gtgctggaca gtgaggggaa taccaatatc
     1861 tccggcgtca cggtggtgtt ccgggcaggt gagcaggagc agacgccgcc ggagggcttt
     1921 gaatcctccg gttccgagac ggtgctgggt acggaagtga aatacgacac gccgattacc
     1981 cggaccatca cgtctgcaaa catcgaccgt ctgcgcttta ccttcggtgt gcaggcactg
     2041 gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag tccgcctgct ggttcagata
     2101 cagcgtaacg gtggctgggt gacggaaaaa gacatcacca ttaaaggcaa aaccacctca
     2161 cagtatctgg catcggtggt ggtgggtaac ctgccgccgc gcccgttcaa tatccggatg
     2221 cgcaggatga cgccggacag caccacagat cagctgcaga acaaaacgct ctggtcgtca
     2281 tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcactggt cggcgtacag
     2341 gtggattcgg agcagttcgg cagccagcag gtgagccgta attatcatct gcgcgggcgt
     2401 attctgcagg tgccgtcgaa ctataacccg cagacgcggc aatacagcgg tatctgggac
     2461 ggaacgttta agccagcata cagcaataac atggcctggt gtctgtggga tatgctgacc
     2521 catccacgct acggcatggg gaaacgtctt ggtgcggcgg atgtggacaa atgggcgctg
     2581 tatgtcatcg gccagtactg cgaccagtca gtgccggacg gctttggcgg cacggagccg
     2641 cgcatcacct gtaatgcgta cctgaccaca caacgtaagg cgtgggatgt gctcagtgat
     2701 ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac gttcgtgcag
     2761 gaccgaccgt cggataaggc gtggacctat aaccgcagta atgtggtgat gccggatgat
     2821 ggcgcgccgt tccgctacag cttcagcgcc ctgaaagacc gccataatgc cgttgaggtg
     2881 aactggattg acccgaacaa cggctgggag acggcgacag agcttgttga agatacgcag
     2941 gccattgccc gttacggtcg taatgttacg aagatggatg cctttggctg taccagccga
     3001 gggcaggcac accgcgccgg gctgtggctg attaaaacgg agctgctgga gacgcagacc
     3061 gtggatttca gcgtcggcgc agaagggctt cgccatgtac cgggcgatgt tattgaaatc
     3121 tgcgatgatg actatgccgg tatcagcacc ggtggtcgtg tgctggcggt gaacagccag
     3181 acccggacgc tgacgctcga ccgtgaaatc acgctgccat cctccggtac cacgctgata
     3241 agcctggttg acggaagtgg caatccggtc agcgtggagg ttcagtccgt caccgacggc
     3301 gtgaaggtaa aagtgagccg tgttcctgag ggtgttgctg aatacagcgt atgggggctg
     3361 aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac
     3421 ggcacgtatg ccatcaccgc cgtgcagcat gtgccggaaa aagaggccat cgtggataac
     3481 ggggcgcact ttgacggcga acagagtggc acggtgaatg gtgtcacgcc gccagcggtg
     3541 cagcacctga ccgcagaagt cactgcagac agcggggaat atcaggtgct ggcgcgatgg
     3601 gacacaccga aggtggtgaa gggcgtgagc tttatgcttc gcctgaccgt ggcagcggac
     3661 gacggcagtg agcggctggt cagcacggcc cggacgacag aaaccacata ccgcttcagg
     3721 cagctggcgc tggggaacta caggctgaca gtccgggcgg taaatgcgtg ggggcagcag
     3781 ggcgatccgg catcggtatc gttccggatt gccgcaccgg cagtgccgtc gcggattgag
     3841 ctgacgccgg gctattttca gataaccgcc acgccgcatc ttgccgttta tgacccgacg
     3901 gtacagtttg agttctggtt ctcggaaaag cggattaccg atatcaggca ggttgaaacc
     3961 acagcccgct atcttggcac ggcgctgtac tggatagccg ccagtatcaa tatcaaaccg
     4021 ggccatgatt attattttta cgttcgcagt gtgaacaccg ttggcaaatc ggcattcgtg
     4081 gaggctgttg gtcagccgag tgatgatgca tccggctatc tggatttttt caaaggcgag
     4141 atagggaaaa cccatctggc tcaggagctg tggacgcaga ttgataacgg tcagcttgcg
     4201 cctgacctgg ctgaaatcag gacgtccatt acggatgtca gcaatgaaat cacgcagacc
     4261 gtcaataaga aactggaaga ccagagtgcg gcaattcagc agatacagaa ggttcaggtt
     4321 gatacaaata ataacctgaa cagcatgtgg gctgtgaagc tgcagcagat gcaggacgga
     4381 cgcctttata tcgcgggtat tggtgccggt attgagaaca cccctgacgg catgcagagt
     4441 caggtgctgc tggcggcgga caggattgcg atggttaatc ctgcgaatgg caacacaaaa
     4501 ccgatgtttg ttggtcaggg cgatcagata ttcatgaacg acgtgttcct gaaacgcctg
     4561 acggccccca ccattaccag cggtggaaat ccaccggcat tttccctgac accggacgga
     4621 aagctgactg ctaaaaatgc ggatatcagt ggcagtgtga atgcgaactc cgggacgctc
     4681 aacaacgtca cgattaatga gaactgtcag attaagggga aactgtcagc caatcagatt
     4741 gaaggcgata ttgtcaaaac agtgggtaag gctttcccgc gggactcccg ggcaccggag
     4801 cggtggccat cagggaccat taccgtcagg atttatgacg atcagccttt tgacaggcaa
     4861 attgttattc cagcggtggc attctgcggc gctaaacatg agcgggagaa taacgatatt
     4921 tattcgtcat gccgcctgat agtgaagaaa aatggtgctg aaatttataa ccgtaccgcg
     4981 ctggataata cgctgattta cagtggtgtt attgatatgc cagctggtca cggccacatg
     5041 acgctggagt tttcggtgtc agcatggctg gtaaatgact ggtatcccac agcaagtatc
     5101 agcgatttgc tggttgtggt gatgaagaaa gccaccgcag gcatcagtat cagctgaatt
     5161 ttataaccca tatacgggcg ccagaaatgg cgcctttttt attgcagaaa agcgagaggt
     5221 aattatgcgt aaagtttgtg cagccatttt gtccgcagcc atctgtctgg ccgtatccgg
     5281 tgcgcctgca tgggcgtctg agcagcaggc cacactgagc gcagggtatc ttcatgcccg
     5341 tacgaacgct cccggcagcg atgatctgaa cgggattaac gtgaaatacc gttatgagtt
     5401 tacggacacg ctggggctga ttacgtcatt cagttatgcc aacgctgaag atgagcaaaa
     5461 aacgcattac agcgataccc gctggcatga agattccgtg cgtaaccgct ggttcagcgt
     5521 gatggcgggg ccgtctgtgc gcgtgaatga atggttcagc gcgtatgcga tggcgggtat
     5581 ggcttacagc cgtgtttcga ctttctccgg agattatatc cgcgtaactg acaacaaggg
     5641 gaaaacgcac gatgtgctga ccggaagtga tgacggtcgc cacagcaaca cgtctctggc
     5701 gtggggagct ggcgtgcagt ttaacccgac cgaatccgtg gccattgata ttgcttatga
     5761 aggctccggc agtggcgact ggcgcactga cggtttcatc gtgggtgtcg gttataaatt
     5821 ctgattagcc aggtaacaca gtgttatgac agcccgccgg ttcaggcggg cttttttgtg
     5881 gggtgaatat ggcagtaaag atttcaggtg tactgaaaga cggcacagga aaaccggtac
     5941 agaactgcac aatccagctg aaagcaaaac gtaacagcac cacggtggtg gtgaacacgg
     6001 tggcctctga aaatccggat gaagccggtc gttacagcat ggacgttgag tacggtcagt
     6061 acagcgtcat tctgttggtg gaaggattcc cgccgtcaca tgccgggacc atcaccgtgt
     6121 atgaagattc tcaacccggt acgctgaatg attttctcgg tgccatgacg gaggatgatg
     6181 cccgtccgga ggcactgcgt cgttttgaac tgatggtgga agaggtggcg cgtaacgcgt
     6241 ccgtggtggc acagaacacg gcagccgcaa agaagtcagc cagtgatgcc agcacatctg
     6301 ccagtgaggc ggcaacccgt gcgactgatg ctgcagattc agcacgtgca gccagcacgt
     6361 cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc cagcgcagga acggcatcaa
     6421 caaaggccac tgaagcctca aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg
     6481 ccaccagtgc cggtgcggcg aaaacgtcag aaacgaatgc ttcagcgtca caacaatcag
     6541 ccgccacttc tgcatccacc gcgaccacga aagcgtcaga agctgccacc tcagccagag
     6601 atgcgtcggc ttcaaaagag gcggcaaaat catcagaaac gagcgcagcc tcgagcgcca
     6661 gtagtgcagc ctcctcggca acagcggcag gcaattccgc gaaggcggca aaaacgtccg
     6721 agacgaacgc taagtcttct gagacggcag cagcacagag tgcctccgca gcagcagact
     6781 caaaaacagc ggctgcctca tctgccagcg ccgcgtcaac aaatgccggg caggcctcag
     6841 ccagtgccac cgctgccgga aagtcggcag aaagcgccgc atcatccgct tcaacagcca
     6901 caacgaaggc tggcgaagcc actgaacagg ccacggcagc agcgaggtca gcttccgcag
     6961 cgaagacatc cgaaacgaac gcgaaagcgt cggaaaccag cgcagaatcc tcaaaaacgg
     7021 ctgccgcatc gtcagccagt tcggcggcgt catcggcatc atcggcgtct gcttcaaaag
     7081 atgaggcgac cagacaagcg tcagcagcga agagcagcgc cacgacggca tccacgaagg
     7141 cgacagaggc tgctggcagt gcgacggcgg cagctcagag caaaagtacg gcggaatccg
     7201 cggcaacgcg cgccgagaca gcagctaaac gggcagagga tattgcatcc gccgtggcgc
     7261 ttgaggatgc aagtacgacg aaaaagggga tagtacagct cagcagtgcg accaacagta
     7321 cgtctgaaac gctggcggca acgccaaagg cagtaaaatc agcctatgac aatgcagaga
     7381 aacgtctgca gaaagaccag aacggcgctg atatacccga taagggatgc ttcctgaaca
     7441 acattaacgc ggtcagtaaa acagactttg ctgataagcg tggtatgcgt tatgtgcggg
     7501 ttaacgctcc tgcaggtgca acatctggaa aatattaccc tgttgttgtt atgcgttctg
     7561 ctggctcagt aagcgaactg gcatcaagag tcattatcac cacggcaacg cgaaccgcag
     7621 gcgatccgat gaataactgc gagtttaacg gatttgttat gcctggtggc tggactgaca
     7681 gggggcgtta tgcttatggc atgttctggc aatatcaaaa caatgaacga gccattcact
     7741 caataatgat gagtaataag ggcgatgatt tgcgctctgt gttctatgtt gatggcgctg
     7801 ctttccctgt ttttgcgttt ctcgaagatg gcctgtcaat atccgcacct ggtgctgatc
     7861 tcgttgttaa tgatacgacc tataagtttg gggcaacaaa tccggcgact gaatgtatcg
     7921 cggcggacgt tatccttgat tttaagagtg ggcgtggttt ttatgagtct cattcgttaa
     7981 tcgttaacga taacttgtcg tgcaaaaaac tttttgccac agacgaaatt gtagcgcgtg
     8041 gtggtaatca gattcgaatg ataggtgggg agtatggggc attatggcgt aatgatggcg
     8101 ctaaaactta cctgctgctt accaatcaag gtgatgttta tggtggctgg aatacattaa
     8161 gaccgtttgc tattgataac gcaaccggcg aactggttat tggaaccaaa ctgtccgcaa
     8221 gtctgaacgg taatgcatta acagcaacaa agctgcaaac gccaagactg gtttctggtg
     8281 ttgagtttga tggttccaaa gatattactt taaccgccgc gcatgtggct gcttttgcca
     8341 gaagggcaac ggatacatat gccgatgcgg atggtggcgt tccctggaat gccgaatcag
     8401 gcgcttacaa tgtcacccgc tctggcgaca gctatattct ggttaacttc tataccggag
     8461 tcggaagttg ccggaccctg cagatgaagg cgcattacag aaatggtggt ctgttctacc
     8521 gttcttcaag agacggttat ggttttgagg aagactgggc agaagtttat acctcgaaaa
     8581 atcttccacc agaaagctac ccagtcggtg caccaatccc gtggccatca gataccgttc
     8641 cgtctggtta tgccctgatg caggggcaga cttttgacaa atctgcttac ccgaaactcg
     8701 cagccgctta tccgtcaggc gtgatccctg atatgcgtgg ctggacgatt aagggcaaac
     8761 ctgccagtgg tcgggccgta ttgtctcagg aacaggacgg cattaaatcg cacacccaca
     8821 gcgccagcgc atccagtacg gatttgggga cgaaaaccac atcgtcgttt gattacggca
     8881 ctaaatccac gaataacacc ggggcgcata cgcacagtgt gagcggtaca gccgcaagtg
     8941 ccggaaacca tactcatagt gtcacaggcg catcagcagt cagccagtgg tcacaaaatg
     9001 ggtcagtaca taaggttgtg tctgcggcca gtgtgaatac aagtgctgca ggagcgcaca
     9061 ctcatagtgt cagcggcaca gccgcatctg caggtgctca cgcacatact gtcggtattg
     9121 gtgctcatac gcactctgtt gcgattggct cacatggaca caccatcacc gttaacgctg
     9181 ctggtaacgc ggaaaacacc gtcaaaaaca tcgcatttaa ctacattgtg aggcttgcat
     9241 aatggcattc agaatgagtg aacaaccacg gaccataaaa atttataatc tgctggccgg
     9301 aactaatgaa tttattggtg aaggtgacgc atatattccg cctcatacag gtctgccagc
     9361 aaacagtacc tatattgcac cgccagatat tcctgctggc tttgtggccg ttttcaacag
     9421 tgatgaggga tcgtggcatc tcgttgaaga ccatcgggga aaaaccgtct atgacgtggc
     9481 ttccggcgac gcgttattta tttctgaact tggcccatta ccggaaaatg tcacctggtt
     9541 atccccggaa ggggagtttc agaagtggaa cggcacagcc tgggtgaaag atgcagaagc
     9601 agaaaaactg ttccggatcc gggaggcgga agaaacaaaa aacagcctga tgcaggtagc
     9661 cagtgagcat attgcgccac ttcaggatgc tgtagatctg gaaatcgcaa cggaggaaga
     9721 aacctcattg ctggaagcct ggaaaaagta tcgggtgttg ctgaaccgtg ttgatacatc
     9781 aactgcacct gatattgagt ggcctacgaa ccctgtcagg gagtaatcat tgggattatg
     9841 ccgcagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc cctgaagcgg
     9901 aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc tctgagagta
     9961 tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc tgattgtaaa
    10021 tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga attgtggctc
    10081 cgtatagagt tccatcgtca gttaaggcct gcgcagcatt gcgtattaca cagctttttg
    10141 tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa tcaaattgac
    10201 catgtaacgc cgcgggataa ggttcaaaaa catcatggct aattttatgt ttaatttttg
    10261 attccccagc ccttgtagat gccgcgttca ggctagcttc gttcaaatcc attaaagata
    10321 tcagactact ctcaggtacg tgagtaaggt aaaacccagt tccaacacca atatccagat
    10381 ggttgttacc taaatgttc
//
LOCUS       DAZFAH010000160        10391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFAH010000160 DAZFAH010000000
VERSION     DAZFAH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.161"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(298..2871)
                     /gene="clpB"
                     /locus_tag="ACHJWB_003703"
     CDS             complement(298..2871)
                     /gene="clpB"
                     /locus_tag="ACHJWB_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4315271.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3001..3732)
                     /gene="yfiH"
                     /locus_tag="ACHJWB_003704"
     CDS             complement(3001..3732)
                     /gene="yfiH"
                     /locus_tag="ACHJWB_003704"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4315272.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3729..4709)
                     /gene="rluD"
                     /locus_tag="ACHJWB_003705"
     CDS             complement(3729..4709)
                     /gene="rluD"
                     /locus_tag="ACHJWB_003705"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4315273.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4844..5581
                     /gene="bamD"
                     /locus_tag="ACHJWB_003706"
     CDS             4844..5581
                     /gene="bamD"
                     /locus_tag="ACHJWB_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4315274.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5852..6193
                     /gene="raiA"
                     /locus_tag="ACHJWB_003707"
     CDS             5852..6193
                     /gene="raiA"
                     /locus_tag="ACHJWB_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4315275.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6296..6421
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6297..6344
                     /gene="pheL"
                     /locus_tag="ACHJWB_003708"
     CDS             6297..6344
                     /gene="pheL"
                     /locus_tag="ACHJWB_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4315276.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6443..7603
                     /gene="pheA"
                     /locus_tag="ACHJWB_003709"
     CDS             6443..7603
                     /gene="pheA"
                     /locus_tag="ACHJWB_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4315277.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7646..8767)
                     /gene="tyrA"
                     /locus_tag="ACHJWB_003710"
     CDS             complement(7646..8767)
                     /gene="tyrA"
                     /locus_tag="ACHJWB_003710"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4315278.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8778..9848)
                     /gene="aroF"
                     /locus_tag="ACHJWB_003711"
     CDS             complement(8778..9848)
                     /gene="aroF"
                     /locus_tag="ACHJWB_003711"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4315279.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10058..>10391
                     /gene="yfiL"
                     /locus_tag="ACHJWB_003712"
     CDS             10058..>10391
                     /gene="yfiL"
                     /locus_tag="ACHJWB_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4315280.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGA"
BASE COUNT         2648 a         2753 c         2441 g         2549 t
ORIGIN      
        1 gccgcttcgc tttttctcag cggcgcgggg tgtgcataat acgctttccc gctacagagt
       61 caagcattta tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg
      121 cgtgtttgcc gttgctccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac
      181 aagcgaaaaa tataaaaact ttatcgttcg ctcatttttc aggcaaaaca tcttaaatat
      241 agtcttttcc gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattta
      301 ctggacggcg acaatccggt cttcattaac ttccaggcga atcactttac ccggaaccaa
      361 ttcaccagac agtatttgct gtgccagcgg gttttcgatc tgctgctgga tagcacgttt
      421 caaaggacgt gcaccataga ctggatcgta accgttctcg ctcagcagtt tcagcgcctc
      481 gtcagaaatg tggatttcat aaccacgttc ttccagacgt ttgtacagac gtttcaactg
      541 aatctgcgca atcgaggcaa tgtgctgttc acccagcgga tggaagacca ccacttcatc
      601 aatacggtta atgaattccg gacggaagtt atggcttacc acaccgagca ccagctcttt
      661 catgtgcgca taatccagtt caccgaagcg ttcctgaatc aaatcggaac cgaggttaga
      721 ggtcataatg acaaccgtat tacggaagtc gaccgttctc ccctgcccgt cagtcagacg
      781 accatcatcc agtacctgca acagaatgtt gaagacatcc ggatgcgctt tttccacttc
      841 atccagcagg atgacggaat acggacgacg acgcacagct tcggtcaggt agccaccttc
      901 ttcataaccg acatatcccg gaggcgcacc aaccagacga gacaccgagt gtttctccat
      961 aaactcggac atatcgatac ggaccatcgc ctcgtcgcta tcaaacataa agttcgccag
     1021 cgccttacaa agctcagttt tccccacacc cgttgggccg aggaacagga atgaaccaat
     1081 cgggcgattt ggatccgcca accccgcacg gctacggcga atagcgttag ataccgcatc
     1141 aaccgcttcg ttctgaccaa tgacgcgatg gtgcagttct tgctccatac gcagcagttt
     1201 ttcgcgctcg ctttccatca tgcgagaaac cggaatcccc gtccaacgcg ccagcacttc
     1261 agcaatttcg gcgtcggtca ctttattacg caacagacgc atagttttgc cttcgagctg
     1321 cgttgcggct tccagttgct tttccagttc cgggattttg ccgtattgca gttcagacat
     1381 ccgcgccagg tcccccacac ggcgagcctg ttcaatagcg attttcgcct gttccagttc
     1441 cgctttaatg gtctgcgtac cagaaagcga tgccttctct gctttccact cttcttctaa
     1501 ctcggagtac tgacgttctt tgtcgctcag ttcttcgttg agcatatcca ggcgtttttt
     1561 actggcttca tcagactctt tcattaacgc ctgttgttcc agtttgagct ggatgatacg
     1621 acgatcgagt cggtcgagtt cttctggttt tgagtcaatc tgcatacgaa tgctggatgc
     1681 tgcttcatcg atcaggtcga tagctttatc cggcagctga cggtcagcaa tgtagcgatg
     1741 agacaacgtc gccgctgcaa caattgccgg gtcagtaatt tgcacatggt ggtgcaattc
     1801 gtaacgttct ttcaggccac gcagaatcgc aatggtatct tcaacagaag gctcggcaac
     1861 aaacactttc tggaaacgac gttccagcgc agcatctttt tcgatgtact ggcgatattc
     1921 gtcaagcgtc gtggcaccta cgcagtgcaa ttcaccacgc gccagcgccg gtttcagcat
     1981 gtttccggcg tccattgcgc catcggcttt acccgcgccg accatggtat gtaattcgtc
     2041 gataaacagg atgacgttgc cttcctgttt ggcaagatcg ttaagcacgc cttttaaacg
     2101 ttcttcaaac tcaccgcgat atttcgcccc agccaccagc gcgcccatat ccagcgccag
     2161 tacgcggcgg cctttcaatc cttccggcac ttcgccgttg ataatacgct gcgccagacc
     2221 ttcaacgatg gcagttttac cgacgccggg ttcaccaatc agtaccgggt tatttttagt
     2281 acgacgttgc agcacctgaa tggtacggcg aatttcttca tcacgaccaa tcaccggatc
     2341 gagtttgccc tgttcggctc gttcggtaag gtcgatggta tattttttca aagcctgacg
     2401 ttggtcttca gcaccttgat cgttcacgct ttcacctcca cgcatttgtt caatcgcttg
     2461 agtaatgttg gcggtggtcg cccctgctgc tttcaggatg tcggccagcg tgccgcgaga
     2521 ctcaagtgcc gccagaacga acagttctga cgagataaag ttatcaccac gtttttgcgc
     2581 cagcttgtcg caaagattaa gaacgcgcac cagatcctgt gatggctgga catcaccacc
     2641 agtaccttca acctgcggta aacgatttaa tgcctgattg atatctgtgc gcaactggcc
     2701 agcatttatg ccagcggatg ttaataaagg actaaccgaa cccccttcct gattcagcag
     2761 ggcgctcatt aaatgaagtg gttcgataaa ttggttgtcg tgcccgagtg caagtgattg
     2821 ggcatcggca agagcaagct ggaatttatt agtaagacga tccagacgca taactcctcc
     2881 cataacggat caaagttgct actggagatt aaatgaggtc atccctcaat tattcaaggt
     2941 tattgaccag attaatgtga aaagaaaatc acgcgtaccg gatcgtcttg attctttagg
     3001 ttatatcagc caaatgaaac ttgccatacg accggtggtc ttgtcgcgac gataagagaa
     3061 gaaagtctca ttttccgtat atgtacaacg gtcgccgccg aaaatttgct caacacccac
     3121 gttcgccaga cgctgccggg caagctgata aatatccgcc agatacttat caccatgctg
     3181 aatgaaagct gcacttgctt tagcgtctac tgccataaac gcctcgcgaa cctccacccc
     3241 cacttcgaac gcgcgtggac caattgccgg ccctaaccag gcgagaatat tttccggatt
     3301 atcagcaaaa caggaaaccg tctcttccag cacgcctgcg cacagtccac gccagccagc
     3361 atgagcggcg gcgacttccg ttcccgctcg attgcaaaac agcacaggga ggcagtcggc
     3421 agtcatcact gcgcaaaccg tgccgggcgt attgctataa gaggcatctg ctcgttttga
     3481 ggcataaggt tcgccagtaa gcttaagcac atctttgccg tgtacctgct caagccagac
     3541 cggtttagaa ggcaaattgc ccgcagcaaa aagtcgcttg cgattctcct caacgtgatc
     3601 cgggttatcg ccacaatggg caccgaggtt gagcgagtca tacgggggca agctcacgcc
     3661 gccgatacga gaagagctac aggccgcaac accttttggt agcggccact gcgggacaat
     3721 cagcttactc ataaccagtc cacttcatcc ttatgctctt cgaaatcggc gcgcatcacc
     3781 tcaatcagct ccaccatatc ttgtggaata ggcgcatgcc attccatttc gatgccggag
     3841 atcgggtgat aaagacgcag catggttgca tgaagcgcct ggcggtcaaa cttacgcagc
     3901 atggagataa atgcttccga agcacctttt ggcggacgcg gacggccacc ataaaccgga
     3961 tcgcccacca gcggatgagt gatatgggcc atatgcacgc ggatctggtg cgtacgtcca
     4021 gtttccagac gcaaccgcag acgcgtgtgc acacggaagt gttccatgat gcgatagtga
     4081 gtcaccgccg gtttgcccat cggatgcacc gccatgtgag tacgtttggt cgggtggcgg
     4141 ctgatgggtt catctactgt accgccagcg gtcatatgac caatcgccac cgcttcatac
     4201 tcacgagtaa tttcacgccg ttgcaaagat tcgactaaac gcgtctgagc cggaacggtt
     4261 tttgccacaa ccatcaggcc agtggtgtct ttatccagac gatggacgat gcccgcacgt
     4321 ggtacatcgg caatgggcgg atagtaatga agcaacgcat tcagtaccgt gccatccggg
     4381 ttacccgcgc caggatgtac caccaggtcg cgcggtttat taatgacgat gatatcttca
     4441 tcttcataga cgatatccag cgggatatcc tgcggttcaa aacgcgcttc ttcttcaatc
     4501 tcagcgttga tggcaacctg ctcgccaccc aatacttttt ctttcggctt atcacaaact
     4561 ttgccgttta ccagcacgcg ctggtcgagg atccattctt ttattcgcga acgtgaataa
     4621 tccgggaaca tttcggccaa agcctgatct aagcgttgac cgagttggtt ttcggacacc
     4681 gttgcagtga gctgtactcg ttgtgccata tatactgctt cttcgtttat cgttggggtt
     4741 ttacggcttt gccgtttaat atagtgtgct attgtagctg gtcttaaccg ggagcaggaa
     4801 cagagaatct cccgtattac attttgagga aagtcaaaac gtcatgacgc gcatgaaata
     4861 tctggtggca gccgccacac taagcctgtt tttggcgggt tgctcggggt caaaggaaga
     4921 agtacctgat aatccgccaa atgaaattta cgcgactgca caacaaaagc tgcaggacgg
     4981 taactggaga caggcaataa cgcaactgga agcgttagat aatcgctatc cgtttggtcc
     5041 gtattcgcag caggtgcagc tggatctcat ctacgcctac tataaaaacg ccgatttgcc
     5101 gttagcgcag gctgccatcg atcgttttat tcgccttaac ccgacccatc cgaatatcga
     5161 ttatgtcatg tacatgcgtg gcctgaccaa tatggcgctg gatgacagtg cgctgcaagg
     5221 gttctttggc gttgaccgta gcgatcgcga tcctcaacat gcacgagctg cgtttagtga
     5281 cttttccaaa ctggtgcgcg gctatccaaa cagtcagtac accaccgatg ccaccaaacg
     5341 tctggtattc ctgaaagatc gtctggcgaa atatgaatac tccgtggccg agtactatac
     5401 agaacgtggc gcatgggttg ccgtcgttaa ccgcgtagaa ggaatgttgc gcgactaccc
     5461 ggatacccag gctacgcgtg atgcgctgcc gctgatggaa aatgcatacc gtcagatgca
     5521 gatgaatgcg caagctgaaa aagtagcgaa aatcatcgcc gcaaacagca gcaatacata
     5581 acagaaacct gaaatacaaa acggcagccc ttgagctgcc gtttttttat tctgttagtt
     5641 gtgaaactga agcgatttag tcactatcga tctcatcaaa tatggctcgc tttgagatat
     5701 tcctcaagta aaaaaacacc tcttcctgcg atttctcaca aaaaagattc gttgacaaaa
     5761 agtgacaaaa ttatgagatt cccatcacac attttgacat caggagcggt atgctgaatt
     5821 caccaagacg ggaagacaag aggtaaaatt tatgacaatg aacattacca gcaaacaaat
     5881 ggaaattact ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg
     5941 gcaaacacat ctgattaatc cacatatcat tctgtccaaa gagccacaag ggtttgttgc
     6001 tgacgccaca atcaatacac ctaacggcgt tctggttgcc agtggtaaac atgaagatat
     6061 gtacactgca attaacgaat tgatcaacaa gctggaacgg cagctcaata aactgcagca
     6121 caaaggcgaa gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt
     6181 tgaagaagag tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt
     6241 gttgacagcg tgaaaacagt acgggtactg tactaaagtc acttaaggaa acaaacatga
     6301 aacacacacc gtttttcttc gcattctttt ttaccttccc ctgaatggga ggcgtttcgt
     6361 cgtgtgaaac agaatgcgaa gacgaacaat aaggcctccc aaatcggggg gcctttttta
     6421 ttgataacaa aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa
     6481 aatcagcgcg ctggatgaaa aattattagc attactggca gagcggcgcg aattggccgt
     6541 cgaggtagga aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg
     6601 cgatttactg gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat
     6661 tactcgcctg ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca
     6721 gcaacatctc aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg
     6781 ttcttattcc catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga
     6841 aagtggctgc gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta
     6901 tgccgtcgta ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct
     6961 gcaacatacc agcttgtcga ttgttggcga gatgacgtta actatcgacc attgtttgtt
     7021 ggtctccggc acgactgatt tatccaccat taatacggtc tacagccatc cgcagccatt
     7081 ccagcaatgc agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag
     7141 tacgtctgcg gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg
     7201 aagcgaagct ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca
     7261 gcaacaaaac ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca
     7321 ggttccggcg aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga
     7381 agcgttgctg gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat
     7441 tcacggtaat ccatgggaag agatgtttta tctggatatt caggccaatc ttgaatcagc
     7501 ggaaatgcaa aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg
     7561 ctgttaccca agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg
     7621 atgtgaatca tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca
     7681 cgcggctttc actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga
     7741 aactgtcaat aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac
     7801 gcttatagta acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat
     7861 aaagctgcgg atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg
     7921 gcgaagagag cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat
     7981 aagcaaaagt agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga
     8041 cagcgctaat acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt
     8101 atgcttccgg cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt
     8161 ccgggccgaa catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct
     8221 gtaatggtcc atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg
     8281 gtaatttgcc gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg
     8341 catcggcaac aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcacctgat
     8401 aacccgatag tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga
     8461 taaccaccgg acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt
     8521 aagattcacg catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag
     8581 cttccgcctc tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag
     8641 gcagtccaaa gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttcgcta
     8701 ataaattcag cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag
     8761 caaccataat aaacctctta agccacgcga gccgtcagct gcccgttcag atcctgatga
     8821 atttcacgca gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca
     8881 ccgtatttca tttcactgcg cggttgctca gaagactgat tgccctcgtg gatattactt
     8941 tcgatcatca gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct
     9001 gccaccgcag gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc
     9061 agagacgggc gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg
     9121 ctatagttcg gcgctttacc accgcgcagg atgacatgcc cgtccggatt cccctgagtt
     9181 tgtagcaacg caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg
     9241 gcgcgcatag cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc
     9301 ggcatggaaa gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca
     9361 attgctgacc agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc
     9421 gtcgccagtg gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc
     9481 ccggcttcta catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg
     9541 acagtggtac ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc
     9601 tctgcggcaa gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg
     9661 gaacaaggac cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg
     9721 gttctacgcg agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc
     9781 agttgttccg gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct
     9841 ttttgcatga tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg
     9901 acgtaacgat aacacataaa gtaaagtttt caatccatat ttcgtacatt tttatttaca
     9961 caggcaattt agtcgcgatt tcaaccctta cttctgtata gataaattta cactcccttt
    10021 aaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc
    10081 cttgttggct ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacttt
    10141 gaccagcacc gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa
    10201 agatgacgat gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc
    10261 cgaaggacaa aagaaaactt gccagaccga ttttacttat gcccgaggac tttccggtaa
    10321 aagctttcct gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca
    10381 aaaaggggct g
//
LOCUS       DAZFAH010000161        10298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFAH010000161 DAZFAH010000000
VERSION     DAZFAH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.162"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..572
                     /locus_tag="ACHJWB_003713"
     CDS             <1..572
                     /locus_tag="ACHJWB_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO4315281.1"
                     /translation="LQSSNSDVVATAAISIAATEMKTILPDDLTPGKYNKIYLSGHGS
                     AGLPLLKCGDEFLSPSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSA
                     NMNNGWLARALFGQKRSLAEHVYAEFERRGINVSISGYHGTGVLYVPEHGKPTTHLRS
                     TTVPATPEHTVRRSDYRATLGRTQPIDIE"
     gene            complement(797..1447)
                     /locus_tag="ACHJWB_003714"
     CDS             complement(797..1447)
                     /locus_tag="ACHJWB_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4315282.1"
                     /translation="MPIVLNFTAGSILPENELASLRYIVQQNQNDIAIIKGRYQMEIR
                     YIESVNGFTVTPVCNNHFSIFMAKQNRIAHHLELQINNGRSFAQISQDFMLQLSSNIG
                     WQKGVENALKNKIHSYSFIVNPDEFSCDTQFLECPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(2042..2290)
                     /locus_tag="ACHJWB_003715"
     CDS             complement(2042..2290)
                     /locus_tag="ACHJWB_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4315283.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2352..3449)
                     /locus_tag="ACHJWB_003716"
     CDS             complement(2352..3449)
                     /locus_tag="ACHJWB_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4315284.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGAQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGNVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQRSRNKTS"
     gene            3538..4575
                     /gene="dusA"
                     /locus_tag="ACHJWB_003717"
     CDS             3538..4575
                     /gene="dusA"
                     /locus_tag="ACHJWB_003717"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4315285.1"
                     /translation="MHDNPETQKTNQTSVMPEKTGAYWSSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            4709..4951
                     /gene="pspG"
                     /locus_tag="ACHJWB_003718"
     CDS             4709..4951
                     /gene="pspG"
                     /locus_tag="ACHJWB_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4315286.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5117..6100)
                     /locus_tag="ACHJWB_003719"
     CDS             complement(5117..6100)
                     /locus_tag="ACHJWB_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4315287.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            6183..7598
                     /gene="dnaB"
                     /locus_tag="ACHJWB_003720"
     CDS             6183..7598
                     /gene="dnaB"
                     /locus_tag="ACHJWB_003720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4315288.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            7651..8730
                     /gene="alr"
                     /locus_tag="ACHJWB_003721"
     CDS             7651..8730
                     /gene="alr"
                     /locus_tag="ACHJWB_003721"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4315289.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            8983..10176
                     /gene="tyrB"
                     /locus_tag="ACHJWB_003722"
     CDS             8983..10176
                     /gene="tyrB"
                     /locus_tag="ACHJWB_003722"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4315290.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2574 a         2392 c         2567 g         2765 t
ORIGIN      
        1 atctacaatc aagtaatagc gatgttgttg ctactgcagc tataagtatt gcagcgacag
       61 agatgaaaac catcctgcca gatgatttaa caccaggaaa atacaacaaa atttatctgt
      121 ctgggcatgg ttctgctggt ctacctcttc ttaagtgcgg agatgaattt ttatcaccgt
      181 cagatattgt cgaccgcatt gttcaacata atcttcatga aatagatgat atcagattaa
      241 catcctgtaa ctcagccaac ataataaaaa acaaagactt ctctcctgat gaaatagaga
      301 aatccgcaaa tatgaataac ggctggttgg ccagggcatt atttggtcaa aagaggtctt
      361 tagcagaaca cgtctatgcc gagtttgaac gtcgcggaat taacgtttct atatcaggtt
      421 accatggcac tggcgttttg tatgtaccag agcatggtaa accaacaacg catctacgct
      481 ccacaactgt gcctgcaaca cctgaacata ctgtaagaag aagcgactac agagccactt
      541 tgggtagaac tcaacccata gatattgagt aattgagcca gcatatacac ttgatggtta
      601 ggaaacagaa tcattgtttt aaacatttcc tggccattaa tattgtcggc gggaacatat
      661 ccaggactgg ccggcaaacc gggtacgcga tctgttgcca tggaaagttg atctgagcgc
      721 tcagtaaata tcaatacggt tctggcgagc cgcttacgat gcatgagaat cactaatagt
      781 atgtaattat tacattttat ttacaatgct gtttctgttg gtctgcatcc ataattatga
      841 aatgctgact tatggtgtca aaataacaca tattcctttc aataatcatt tcttttacta
      901 tcttttccct gctgagaggg tggggtagat gttctcttgt gagattcatg aaagcagact
      961 tatcataaag agtgcatatg ttggagttca gtgcgttctt gacaaaaacc cctttttctg
     1021 gaacacataa tgttattggg cactcaagaa attgtgtgtc gcaagaaaat tcgtcaggat
     1081 ttacaataaa tgaatacgaa tggattttat ttttaagtgc gttttcgacc cctttttgcc
     1141 atcctatgtt tgaagataat tgaagcatga aatcctgaga tatttgcgca aaacttctcc
     1201 cgttgtttat ctggagttcc aggtggtgag caatacggtt ttgttttgcc ataaagatgg
     1261 agaaatgatt attgcataca ggggttaccg taaaaccatt gactgattcg atataacgga
     1321 tctccatttg ataacgaccc tttatgattg caatatcatt ttgattttgc tgtacaatat
     1381 aacgtaaaga tgctagttca ttctcaggca agatactgcc tgctgtgaag tttaaaacga
     1441 taggcatgtt ttttctgtaa tacaagtcga ttatttgtga tttcgcgctg taggtatatt
     1501 atcattcaga cttaacctcc taaagtatga tctagtaggg aggcaaatgc aatggttaaa
     1561 gggtgattgc gatgataaat tatcgcttaa caaatccaga attacagaaa gagataagca
     1621 gtgtgttttc catatgccaa gagatgtaag ttgcaactgt attatatgtc agaaaatagg
     1681 gagtatctgg atgcaggtag ctctgtgtaa tgtattcaag agattaaggc aatcgaaata
     1741 attggcataa aaatattgca taggtgaggc ttattgctca gaagatagag ttatcgatac
     1801 agaaactgga agaactgcgg cagcgataac gcaggtcgat cgcaatgacg agatttatca
     1861 gcgccagcgt gggataaaag agaagctgag cagtctggag aatttgaaat cggttaggga
     1921 tttcatacta gaataatagg atctgcggca cgtcgtatgc aggacgtgct gcggcaggtt
     1981 gatggacgtt cgatcgcgcg agtttgaatg atttccagcc gttaccgatt ttacgtgttt
     2041 attagtgaac aaaccactcg tcagcagatt cccaggtatc tttcagcgtt tcctgaacaa
     2101 aagtttttgc agaatcttta tctgcggtgc gcgtaacaga aaggccatcg ttgctggtgg
     2161 cttttacgat cacctctaca tcgtcataac gcttactgat gcgccgagtt aattcttcct
     2221 ttaacgcatc cacagcaccg tttggcattt tagtcatttt ctctttggct atgcagattt
     2281 caatacgcat aaaaatccct ctatactgtg tttgtataca gtattatttt tatctgtata
     2341 aataaacagt gtcaagaggt cttgtttctg ctcctttgga gttcttcaaa acgattatgt
     2401 aaagatttcg gatacagttc ggtatatacc tgccatagca cgtttaatga acgatgccct
     2461 gtaacttgag cgacttcctc aatactaaaa ccagcctcaa ataagcgact tgccccttct
     2521 ctacgcaaat catggtatcg cagatcttta atacctaatt tgcttcttac cctctgaaat
     2581 cctgcggtaa cagaagtgct gttatatgga aaaatgaatt ctgatttttt gggttgtcgc
     2641 tggacgatat cccaggcttc cccaagcaag gcaactttca tatggttacc ttccttttta
     2701 cgtggatctt tcctgtctct tactagtata gatttttgtt cttggtcgag atcttcccat
     2761 cgtaaccggc atacttctcc gattcgcata caggaccaca cagaaaattt gaggatatca
     2821 acgaacggaa tttttgagca tttatgagta gatcgttgtt gaaggccttc aatgagcatg
     2881 tccagttcat cagatgctgg tctacgatta cgacggtttg atttaccaat caaaccgagt
     2941 ttaagtagat atggacgagc gcttttcgcc gggtttgatg tgtaattgat tccgtataca
     3001 ggttttgccg catccagaac attgccaaga taactaacat cgtggctgac tgttgctggg
     3061 cctgcaccag cgttgtttct tagcctgcaa tgttcaatta cgtcattttc tgtcagttca
     3121 gatagtttga tcgcggatat gtcactatcc ataagcagtt ccagcacata tcttttagta
     3181 cggcctgctt tacctccggc atttgggtca tttaaatatt tgtgtagtaa gtcacggact
     3241 gtaagtccat caacagcatt tgatgatgga atgccatata gatctaattc catcactttc
     3301 tgtgcgcccc atgttttggc atgagcatgt ttagggaatg ttttgctttc cctgtaagtg
     3361 ataacacctt tttctttgat aatcacatta cagcgatagc gtggtgtgcc atcggatttt
     3421 agtcgtttct ctatgttata gtacgccatt acacgacctc gttatttcgg gttcccataa
     3481 aacgtgggaa cctgtgcggg aacctaacgc gagaaaaata gcctgaaatg ttcaaaaatg
     3541 cacgataatc ctgaaacaca gaaaactaat caaaccagcg tgatgcctga aaaaactggc
     3601 gcttactgga gttctcggtt tagcattgct cctatgctcg actggacgga cagacattgc
     3661 cgctatttct tgcgtctgct ttcccgcaat acgttgcttt ataccgaaat ggtgaccaca
     3721 ggggcgatta ttcacggtaa aggtgattat ctggcgtaca gtgaagaaga acatccggta
     3781 gcgttgcaac tgggcggtag cgatccggcg gcgctggcgc agtgtgcaaa gctggcagaa
     3841 gcgcgcggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
     3901 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg
     3961 atgcgcgatg tggtgtcgat tccggtgacg gtgaaaacgc gtattggcat cgacgaccag
     4021 gacagctatg aatttctctg cgatttcatc aataccgttt ccggcaaagg cgagtgtgag
     4081 atgttcatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
     4141 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg
     4201 acaatgtcga ttaacggtgg tatcaagtcg ctggaagagg ccaaagcaca cctgcaacat
     4261 atggatggcg tgatggtcgg gcgcgaggcg tatcagaatc cgggtattct ggcggcggta
     4321 gaccgggaga tctttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc
     4381 atgtatccgt acattgagcg tgaactcagc caggggacgt atctcggcca tattacccgg
     4441 catatgctgg gtttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     4501 gaaaatgccc ataaagcggg tgctgacatt aacgtgctgg aacacgcgct caaactggtg
     4561 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     4621 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgccag
     4681 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     4741 tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     4801 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     4861 actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aaatgccgaa
     4921 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     4981 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     5041 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     5101 ggaagcccta tttcttttat ggaatcaaca ggctggaacc ttgcgtcgcc cggctttcca
     5161 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     5221 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     5281 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttttgat
     5341 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctgaccatta
     5401 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     5461 acaccacgcg cactttctta ccgccggtga tctcttttaa ccgctcgacc agatcctctt
     5521 cacgatagtt aataacctgc cacgcgcccg cttttagcgc gctctgcgct ttttgcgcgg
     5581 ttcctacggt gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaattaagc
     5641 caacgccgcc agccgctgcg tggaacagga actgttcatc gggtttaatt tcataggttt
     5701 tgcgcagcag ataataaacc gttaagcctt tcaggaagga tgccgcagct tgctcaaaag
     5761 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     5821 cgcctaacgc cgactgcgca tagactacac gatcgcctgc cttaatatgc tttacaccac
     5881 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agtgatggcg
     5941 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     6001 cgacctggat ttcattctct gccggatcgg caggagtgaa ctctacggct tgaagtactt
     6061 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     6121 tcagggtctg cttcatatgg taaagtttcg acccattctt tatctcggta actccattca
     6181 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     6241 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     6301 gtttaatgct ggataacgaa cgctgggacg atgtagccga gcgtgtggtg gcagacgatt
     6361 tttacacccg cccacaccgc catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     6421 gtcctatcga tctgattacc cttgcggaat cgctggaacg ccaggggcaa ctcgatagcg
     6481 tcggtggttt tgcttatctg gcagagctgt caaaaaatac gccaagtgcg gcgaacatca
     6541 gtgcttatgc tgacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     6601 atgagattgc tgaagccggt tttgatccgc aggggcgtac cagcgaagat ctgctggacc
     6661 ttgctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc aaacaaagac gaagggccga
     6721 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagttg tttcagcagc
     6781 cacacgatgg cgttaccgga gtaaacaccg gttatgacga tctcaacaaa aaaaccgctg
     6841 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     6901 ttgcgatgaa cctcgtcgaa aacgcggcga tgttgcagga taaaccagta cttatcttct
     6961 cgctggagat gccttcagaa cagattatga tgcgttctct ggcgtcgctg tcgcgcgttg
     7021 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     7081 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcctcc ggcctgacgc
     7141 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     7201 tcatgatcga ctacctgcaa ctgatgcgcg taccggcgct ttccgataac cgtacgctgg
     7261 aaattgcaga aatctctcgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     7321 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgc ccggtcaact
     7381 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc
     7441 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     7501 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     7561 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     7621 attaaagcaa acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     7681 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cccctgccag taaaatggtt
     7741 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     7801 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     7861 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     7921 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     7981 gctagcctgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     8041 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacccagtg caaaaacgtt
     8101 cgtcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     8161 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaacctgg tcaacgttcc
     8221 attgccgcat cgggtggcat tctgctgtgg ccacagtcgc attttgactg ggtgcgcccg
     8281 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccaccggtgc cgattttggc
     8341 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagccgga
     8401 gaacctgttg gttatggtgg aacctgggta agcgaacgtg atacccgtct tggcgtagtc
     8461 gcgatgggct atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg
     8521 aacggtcgcg aagtaccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta
     8581 ggtccacaag cgcaggacaa agccggggat ccggtcattt tatggggcga aggtttgccc
     8641 gtagaacgta tcgctgaaat gacaaaagtg agcgcttacg aacttatcac tcgcctgact
     8701 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgttt gtctgtgccg
     8761 gatgcggagt gaatgccttg tccggccaat aaaatcctac caattcaata agttgatatt
     8821 ctttcatgct cttataaagg tcgagcctct ggcgaatgta cgtttgtcat gggtctcact
     8881 ctgttgctaa ttaccgttcg ctcctgaaca tccactcgat cttcgtcttc ttccggttta
     8941 ttgtgtctta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     9001 gcctacgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     9061 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaactg
     9121 aaagccgtgg cggaggcgga agcgcgcctg aatgcgcagc ctcatggcgc ttcgctttat
     9181 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcg
     9241 gaccatccgg tactgaaaca acagcgcgta gcaaccattc aaacccttgg cggctccggg
     9301 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     9361 agcgatccta cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtgagt
     9421 acttacccct ggtatgacga agcgactaac ggcgtgcgct ttaatgacct gttggcgacg
     9481 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     9541 ggtgccgatc tcactaatga ccagtgggat gcggtgattg aaattctcaa agcccgcgag
     9601 cttattccat tcctcgatat tgcctatcaa ggatttggtg ccggtatgga agaggatgcc
     9661 tacgctattc gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg
     9721 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttatgtg tgaagatgcc
     9781 gaagccgctg gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     9841 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     9901 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaactg
     9961 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
    10021 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
    10081 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgta
    10141 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggagctac
    10201 ccagcctgca gtgaaattaa actgtcgtcg ctttcactct ttctttatag atgatttttt
    10261 tgatgccatc gttctacgtg agagataata aacgttat
//
LOCUS       DAZFAH010000162        10051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFAH010000162 DAZFAH010000000
VERSION     DAZFAH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.163"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..220)
                     /locus_tag="ACHJWB_003723"
                     /pseudo
     CDS             complement(63..220)
                     /locus_tag="ACHJWB_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            298..1392
                     /gene="ddlA"
                     /locus_tag="ACHJWB_003724"
     CDS             298..1392
                     /gene="ddlA"
                     /locus_tag="ACHJWB_003724"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4315291.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1416..1628)
                     /locus_tag="ACHJWB_003725"
     CDS             complement(1416..1628)
                     /locus_tag="ACHJWB_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4315292.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1888..2196
                     /locus_tag="ACHJWB_003726"
     CDS             1888..2196
                     /locus_tag="ACHJWB_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4315293.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2255..3349)
                     /gene="yaiW"
                     /locus_tag="ACHJWB_003727"
     CDS             complement(2255..3349)
                     /gene="yaiW"
                     /locus_tag="ACHJWB_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4315294.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3362..4582)
                     /gene="sbmA"
                     /locus_tag="ACHJWB_003728"
     CDS             complement(3362..4582)
                     /gene="sbmA"
                     /locus_tag="ACHJWB_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4315295.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4934..6091
                     /gene="ampH"
                     /locus_tag="ACHJWB_003729"
     CDS             4934..6091
                     /gene="ampH"
                     /locus_tag="ACHJWB_003729"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4315296.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYIQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6092..6715)
                     /gene="iprA"
                     /locus_tag="ACHJWB_003730"
     CDS             complement(6092..6715)
                     /gene="iprA"
                     /locus_tag="ACHJWB_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4315297.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6803..9806)
                     /gene="ehaB"
                     /locus_tag="ACHJWB_003731"
                     /pseudo
     CDS             complement(6803..9806)
                     /gene="ehaB"
                     /locus_tag="ACHJWB_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
BASE COUNT         2598 a         2483 c         2378 g         2592 t
ORIGIN      
        1 gaacaggcct ttaattatca acctttatta taaacaaaag ttatgaaata tattctctgc
       61 gtttacttac aactaagaga aaaaactcac cgcatggcca tttaagccag gaataaatta
      121 gcttcccgtt catcattttt gtgatctcgc gcacatcttt gtgataacgc actttcttca
      181 ccattacgtt gcacgctttg tcagcagaag attgcaacat tctgtcagaa ggccgcgaat
      241 cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg
      301 gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg
      361 caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg
      421 ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct gctaaatgca
      481 gacgatcctg cccatattgc gttgcgccct tcggcgacca gccttgcgca ggtgccaggt
      541 aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc
      601 attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg
      661 gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa
      721 gatgtcacca aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg
      781 acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca
      841 ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa
      901 gaacagtacg caattgccgt cgatctggcg ttcgaattcg atcataaagt gatcgttgag
      961 caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa cccgcaagcc
     1021 agcacctgtg gcgagatcgt actcaccagc gacttctatg cctacgacac caagtacatt
     1081 gacgaagatg gcgcgaaagt ggtagttccg gcagccattg cgccagaaat caacgataag
     1141 atccgggcga ttgccgttca ggcttatcaa acgttgggat gcgcaggcat ggcgcgtgta
     1201 gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc
     1261 ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat
     1321 ctgatcacgc gtttgattga gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa
     1381 accacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca
     1441 tcgcgacgac gaataatcag ccccgccagc cagaaactaa tcacccacgt caccagaccg
     1501 acggcatagg tctgccaacc ttttgcttca aatcccagta accccaccac gccattaaga
     1561 atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc
     1621 agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt aacaaccgtc
     1681 tgtggtgtag acaacatttt cgtaattgtt aataaattta acttaaggtt gccattttgt
     1741 gactcacaac gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg
     1801 atattgacaa tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag
     1861 agaagcagat gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt
     1921 agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg
     1981 ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc
     2041 tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt
     2101 gtcggcacca ttttcgggct gatcccgttt ttagtaggct gcctcatttt tgcagtggtg
     2161 gcgctatggc tgcactggcg acatcgccgc cagtaagatt tacgttgcca gatacggcct
     2221 aaaacgcctt atctggcatg acattcctgt ttgatcactg tttcatacaa cgcgcccgcc
     2281 gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt
     2341 ccagttgaat gccaggcaac atttcgcgag gtagtgattt acccgttttc gcttcggcaa
     2401 gttgataaac tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta
     2461 actgacggcg aatttcgctg tcgctcatcc ccagttttcc cgccagtttg cgcgttgcca
     2521 gttcggtttt cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct
     2581 tcacgccgct ggccttactg actgcgttct gaaacgcggc attacggctg gcgtaccagc
     2641 cagcgttaaa atcagcaaag cggtatatcg gtgcgctata gctggcggga taattcagta
     2701 aatggtaagt accaaaccac aacccgccgc ggcgactgaa gacttcctga cgaactgtac
     2761 cgtccatttt ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca
     2821 tcggcccacc ggtgcgcacc ggattaagcg aaccaaacag cgtctgcccc attggtacca
     2881 tgctgatcag atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc gaatctaacc
     2941 gttcgctata acttttgccg tttggcgatt tgatttttag cgcggtatga accagaaaag
     3001 caggaatgtg catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc
     3061 ccggaacggc cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagc
     3121 agacgttttc cagcgtcggt gccagcccct gactttcaaa ggtggtggcg agatctttcg
     3181 cccaggcatc gcggtctttc acgctcgcag gcatcttctg ccgcacgacg ctcgctacat
     3241 ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg actactacac cccgctaaaa
     3301 ccagaaccgc caacaatgac agcgaagata gatgattgac gcgcgacatt acttctcctt
     3361 tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc atccagctca tgttcaaagc
     3421 tgcgcagacg tttgtagata gacatcaact caaccagtgt ggtccatgag ttaatcaggt
     3481 actggaaagc accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc aggccgagcg
     3541 taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg ttatcgacct
     3601 gcaaatagag aatgcgggcg atgttgaaat acatatagtg aaaatagagg cgaaaatagt
     3661 ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc gtggcatcgt
     3721 cttcaccata aaccagctct ttacggtagg cggcctctac tcgctggttt ttaaactcca
     3781 gccccggcag tttgatccct accactgcca gcaatccggt ccccatcagc gaccagacga
     3841 tagcggcaat caccagacca tacggaatgt gcccgataat cggcagctcc ggcacatgcg
     3901 cggagagcgt taccagcacc ggcagaaagg cgatcaacgt catgatggcg ttgataaaac
     3961 tgacgcccat attctccagc gttgaagcaa aacgcatggt gtcttcctgc acacgctgtg
     4021 cggccccttc gatatgacgc agttgttgcc agttcgccat gtaatattcg ttcatcgccg
     4081 tacgccagcg gaacacatag tgactgacaa agaagttgtt cagtacactg atcaccaccg
     4141 cgatcagcgc aatccccaga aagacgccca cttcgcggta aaattgttcg atagtgactt
     4201 tatgcggcga acttagcgcg gtttgaatca gatcatagaa cggtgcatac caggcgttga
     4261 cggcgacccc gacttccacc aaaaaccagg tgacgaagat gatcagtgca gtaccgagta
     4321 ttgaccagta ttgccaacgg tgcgggctgt agataaacca gaaaaatgca aaaagtccta
     4381 cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc
     4441 taatcgggat ctgcccggaa gcgccggtga tacgcgccac ccagtcaccc ccaccggctt
     4501 gccagaagat aacggcaatc aatgcccaaa caaaggccga gagaaaaaac gttcccggct
     4561 ttgggaaaaa agacttaaac atatgcactc ctgctaactt cttatcgttt taacaactgc
     4621 tgtgtattaa ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc cattgtgacc
     4681 catagcaagg cgcttagttc gccagcctga ccataagaat tgtttcgacg gatttcacaa
     4741 accaaagatt gtttacataa gcggattaac acgcgcagga tcacaaatgc aggcattggt
     4801 gagtaggaaa aagatcgtgt ttacgcgccg ttacgttttt aacactatta ttgcactaat
     4861 tcgccctttg caatccatca atgagtagta taaatacgct cagttacctt cattcaatct
     4921 atggacacca ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg cggcgtcatt
     4981 gacctccgtc catgcggcgc agccgatcac tgaaccggag ttcgcctctg atattgttga
     5041 tcgttatgcc gatcatattt tttacggcag cggtgccacg gggatggcgc tggttgttat
     5101 cgacggtaac cagcgcgtct ttcgcagtta tggcgaaacg cgacctggta ataacgttcg
     5161 cccgcagctg gattcggtcg tgcgtattgc ttccctcacc aagctaatga ccagtgagat
     5221 gctggtgaaa ttgctcgacc aggggaccgt gaagttaaac gatccgctaa gtaaatatgc
     5281 tccgccaggc gcacgcgtgc caacctacaa tgggacgccc attacgctgg tcaatctggc
     5341 aacccatacc agcgcgctgc cccgtgaaca gcccggtggc gcggcacatc gtccggtgtt
     5401 tgtctggcca acgcgcgagc aacgctggaa atacctttct acggcgaagc tgaaagccgc
     5461 gccaggcagt caggcggcgt actctaacct tgcgttcgac ctgctggctg atgcactggc
     5521 gaatgcgtcc ggcaagcctt atatccagtt gtttgaagag cagattaccc gtccattggg
     5581 gatgaaagac accacctaca ccccctcacc ggatcagtgc cgccgtttaa tggttgcaga
     5641 gcgcggtgcc agtccatgca ataacacgct ggcggcaatt ggcagcggcg gcgtctattc
     5701 cacgcctggc gatatgatgc gctggatgca gcagtatttg tcatctgatt tttatcagcg
     5761 cagtaaccag gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt ttacgaaagt
     5821 gattggcatg gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg tatatatggc
     5881 cccgaaagag ggtcgtccgg ggattattca gaagacagga ggtggtggcg gtttcattac
     5941 ctatatggcg atgatcccgc agaagaatat tggcgcgttt gtggttgtca ctcgttcgcc
     6001 gttgacgcgc tttaaaaata tgagcgatgg catcaatgac ctggtgaccg agctaagcgg
     6061 gaataaaccg ctggttatcc ccgcgtcctg attaatactc tgaaggcaaa cggttgatag
     6121 cgaccagttt gcctttattc atttcgatat agccgccttt acgcaaagca gcgagaactt
     6181 ctgcgacgac agaacgcgat atgcgtgtac gttgatggat ataattcatc acaccaatac
     6241 gtgagcgcaa ttcttcattc cagtcaatca ttgataataa tgttgcgcgg atttgttcgt
     6301 aggaattatg cccaatgagc tgcacgtcgc gtaattccag aattctattt tgccaggcta
     6361 accagtaaaa agcgtctcgc cagagttgat tttgttcaat aagcgtaatg gtttgtttgg
     6421 ctggtagatg atatcccgta caatttcctt ctgatattaa tttgtatggt atatcgtttt
     6481 tcattaaacc atcagccagc cccataatat aaggagcctg ggtaataccg ataagtacgt
     6541 tttcttctct acgcagagag ataacgccct ctaaaataac gaaagtatct tcgctattga
     6601 catcactgga aaatataact tgcttttcat tattaaactc gaagcgcgta ccgtatctgg
     6661 acaaacattt atcgagctta ccaaattcct gaagaggttt aactacagat aacatttgcg
     6721 cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat tatttagatt aaattaccag
     6781 aatttcttcg gagtgaagaa tattaccagg tatatttaac acccacgttc gcggaccagt
     6841 cttgatctac gtcaccacca ccgaggtagt tagcatcggt ataggcgctg aagttcttgg
     6901 tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga cccttcagta ccgttatcga
     6961 tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa gccagtttga
     7021 agtacggagt cagagcctgg tcttcgctgt aggtgaaggt ataacctgca tctacaccca
     7081 gttcataacg catgctgtcg taagactgac cgtcaacttt catgtcgttg ctcagctggt
     7141 agtcatcacc agactggaac agaccagaaa cgctgccgta aggagtcacg taaccagcat
     7201 cacccagttt gaagtcgtaa ccggctttca aaccgaagcc ccacgcgtca gagttggtgc
     7261 taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg aagtgagagt
     7321 agctcaagct accatcaaca aagacgttgt tcgcgaagtg agcagaagag tagatgtagg
     7381 cagtctggct gtcttgatcc acctgaccag aacggtcatt catgtcacct ttagcgaagc
     7441 ctgcagccgc accgacgatc cacttagcgt tgttaccgtc aattttggta tcaacaccga
     7501 ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc gttgtcgccg ttgaagttac
     7561 caccgaagta gcttacccat gcgccgccgt tatcagccag gccatgacga gagttggtca
     7621 gacgagtacc aacggtgtct tgttccaggt tccagatatt ggtgttcgca gacgggatgc
     7681 tcagcgccat gttagcgtag tcggtcagct ccatctgttg cagaacaacg gtgttaccgc
     7741 gctgttcagc ctgataggtg tatgcaccca ggtcagcttt gttagcagca gagaaggtcg
     7801 cgttgctgtt tttgtcgttg acgtagatca gctcgttgcc gttgtagtca gcgatagaac
     7861 cagcaccagt tgcgttgtcg atacgaactt tgtagttacc ggttgcagct gcaacatggt
     7921 caccgttgtt atcaacttcg ttctgaccag cttcgataga agcagtgttg tcgttatcgc
     7981 cgttaccgtt aatggtcagg tgaccatcag agttcattgc gattaagcca taaccgtagt
     8041 ttgacttggt agtatcgttg gtacggtcgt tggtcaggtc tgcatcaagt acgaagcggt
     8101 tgctttcgat gttgaacaca ccagcgtgaa tgttaccact ggtatcagta gtgctggtca
     8161 ggttcagagt atcaacctgc agagcattcg ccgcagtgtt acctacatcc agaataccgt
     8221 cgttagtcac tttgatgttg ttggcataca gagcagcatc acctacagcc caaccagcag
     8281 aaacttcatc agaaatggtt acggtactgt tgtcgacagt cagtgaatca gttgcaacat
     8341 gaccatcttc gttgatgttc agcgcagaaa gacctgtcag agagatagag tcagaaatca
     8401 gcgttgaatc agcaacatta acagttgaac cactgttgat gctcagagta tcaatcagag
     8461 aagttttggt ggtatcccac tgtgaaccgg tattcagggt cacattgaac agaccgctct
     8521 ggtaaacctg attaccagta atatgatcga attcactgta acttgtatca tcgttttcaa
     8581 caccaacggt tgataacggc cacaggctat tatcctcaat atcaatcaga gttgcagttg
     8641 cctcggtgcc aacaccgtat gtagcgatgt cacctttacc atctccatca acatcaatag
     8701 aaccagtttg atgaacagat tgtgctgccc ctacccattt actaccgtta tttaatgtca
     8761 gatccaggcg atctgttcca tcccaaccgt tggtgtctac ttcaccatca gcatcgcgat
     8821 aactatctgc accacgtagg aagaagtttt catcaaagtt gctagagaag attacatcgc
     8881 ccatcaaagt agagttacta aagtcagcgg tggtctgcat agcattatca gcattcggat
     8941 gtgcaactac agctaatgca accatcatcc cagcttgcaa tagtgctatc agcaacacca
     9001 tctccgtcag tatctacggt gacaaagtta gatttaccac tataatcgct ggcattacct
     9061 gtattaccga accaaccagt agtaccttca tcagtccatg aaccagaggt tacggtagaa
     9121 tctttaacag tgatggtgtt attaaacact tcgctgctgt ttacgccagt gctgacagtg
     9181 ttgtcgtcaa tatcttccca ctcataaccc tgagtcaacg taatacccgc aacgtgagag
     9241 ttattagtga tggacagatc aacttcctga tccagagtaa tagccgtacc aatgttgtaa
     9301 acgtctacaa catgagtatc agcggcatta ttgtaagtgc cgttatatgt gtagtgttcg
     9361 tagttgtcgt cgatcgttga attatcaaca gtcagcgcca gacggtcatg gtagtaatca
     9421 tccgcacgat cagcacaatc agttgtcatg cactcggaat aaatcatacc gtgaatagtg
     9481 ctgtttttga ttgtcagact attggcgttg gtattggtag aaataccgtc gtccagatag
     9541 taggtagaaa tcacgccgtt aacggtagcg ttattaatga ccgggtagat atcgccgttg
     9601 taatagctat cagcggcata gttgttccag cctacataac catcatagta aacgtcatca
     9661 gcataggtgg cgtcgttgta gtgatggaaa gtattgtaag tttgaccaga aatatcgttg
     9721 gtcgctgcat tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact
     9781 acaagtttct ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat
     9841 ccatcaatta tcaatgcata gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt
     9901 acagacgaat ggttcaatta ttctttcccc taagtccgaa tgtggacata tataatttat
     9961 ggagaaaaac acgcatcaaa ttggtaaaag gacgatattt caacatgttg cagataatgt
    10021 tatttttgat acatacgcgt tataaatatc a
//
LOCUS       DAZFAH010000163         9877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFAH010000163 DAZFAH010000000
VERSION     DAZFAH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.164"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..614)
                     /locus_tag="ACHJWB_003732"
     CDS             complement(<1..614)
                     /locus_tag="ACHJWB_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4315298.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEI"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHJWB_003733"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHJWB_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4315299.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2493..3554)
                     /locus_tag="ACHJWB_003734"
     CDS             complement(2493..3554)
                     /locus_tag="ACHJWB_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4315300.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3796..5907
                     /gene="pqqU"
                     /locus_tag="ACHJWB_003735"
     CDS             3796..5907
                     /gene="pqqU"
                     /locus_tag="ACHJWB_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4315301.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNITTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFELLP"
     gene            complement(5934..6599)
                     /gene="mcbR"
                     /locus_tag="ACHJWB_003736"
     CDS             complement(5934..6599)
                     /gene="mcbR"
                     /locus_tag="ACHJWB_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4315302.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6797..7834)
                     /gene="curA"
                     /locus_tag="ACHJWB_003737"
     CDS             complement(6797..7834)
                     /gene="curA"
                     /locus_tag="ACHJWB_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4315303.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8015..8533
                     /gene="mddA"
                     /locus_tag="ACHJWB_003738"
     CDS             8015..8533
                     /gene="mddA"
                     /locus_tag="ACHJWB_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4315304.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8530..8979
                     /locus_tag="ACHJWB_003739"
     CDS             8530..8979
                     /locus_tag="ACHJWB_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4315305.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8980..9213)
                     /gene="ydcY"
                     /locus_tag="ACHJWB_003740"
     CDS             complement(8980..9213)
                     /gene="ydcY"
                     /locus_tag="ACHJWB_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4315306.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9299..9472)
                     /gene="ortT"
                     /locus_tag="ACHJWB_003741"
     CDS             complement(9299..9472)
                     /gene="ortT"
                     /locus_tag="ACHJWB_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4315307.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9667..9762
                     /gene="yncL"
                     /locus_tag="ACHJWB_003742"
     CDS             9667..9762
                     /gene="yncL"
                     /locus_tag="ACHJWB_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4315308.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2364 a         2429 c         2427 g         2657 t
ORIGIN      
        1 ataatttcat tcctttttaa tacttcttgt aatttcggta actgacatac agcatcagca
       61 atcgcactga tattcgtggc attatcctga aaccattcat gcccaggccc ccatgtgcgc
      121 atagtgcaaa gataacagtc caccagcgtt agttgctcac caaacgcata aggttcagcg
      181 gtcaattgtg aattcagcca gatataaagc gatttacgat attcaataac attcttcttt
      241 aactgttcag gtgcgtcagg agcccaacgc tcagggtaat cggcgaaagt gaatgttgga
      301 tagacattag caaccagcca gaccaatagc cgttgaaact gctgacgttc ggcgcgccca
      361 accggtggcg caaggtccgg gcgacgatcg agaaccatca atgcaatcgc cgctgtctcc
      421 gtcataatct cgtcattttc cagcgccagt gtcggaatct gacacaacgg gttcagggtt
      481 ttcaataact cgcgtgaagt tccctcgtgg tcaaaaccac tgacatcaac gaactgataa
      541 ggaatatcag ccagtgttag catcagctca ctgattgtag agccccatcc gggtacgcca
      601 tacaccttaa tcatatgcct ctcacaatct tttaaagatt aagtgtagga cagtacacag
      661 gttaaccagc aaaaggattt gtcatttttt cgtcagttgt attaatcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcactgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcgctgacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacagtg ttcctcggta gcggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aatcaccgat aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagcgat taacagtgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gttattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaac tcaggcctgt actgcaccgg
     1861 gcgtattctg cgctcgatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacactg gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcaat
     2101 taaacaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct ggtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagttaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aattcagatt actgacgaag gtgccggatg cggcgtgata aaagtcaggc
     2461 aagtctctcc gttttcaccc tcgccatatc tattacagcg caatacgaat cacatcgtct
     2521 ggctgggtag cttcctgctg tttagtggat ttttgtttca cactgacata cagcgttttg
     2581 ccatcggcag acagcgccag gctgtttgga tgagtcggcg tatcgaacgt tttcaccact
     2641 ttatagcttt tcgcgtcaat cacactaact ttacctgcct gacgatgcgt tacgtaggct
     2701 tcattacgcg ctgggttaaa cagcacagcc agtgattccg gtgccgcaac cttcgccaga
     2761 atattgccat tacgggtatc gaccactaac acttctgcgg ctttagaatc ggtgataaat
     2821 gcacgctgcc tggcggtatc aaggctaatg ttgataaaga agtgctcttt gccgtcatcc
     2881 agcagctttt tacggctgag gattttattg tcggcggtgt cgatggtaat caattcgccg
     2941 tcagcgttag tggtgtaaag acgtttgcct ttgctatcca gcgccagacc ggtactcatt
     3001 ttaccggtgt tctggatggc ggttttcagt ttgatattct cgccatcaac gacccaaatc
     3061 acgctatctt taccaatacc actgatgtaa acggtgttcg tggcatcatc agctaccagc
     3121 tcacgcggtt gcagcgggcg cacctcttcc gtgcgcttac gatcatccag caccagacgg
     3181 cctttcactt cgcccgtttt ggcatctatc gccgtgaccg cgctgtttac ggtgttacca
     3241 aaccacaacg tctgagtcgt gttattgatg gtggcaccaa acggcttgag atcgttatgg
     3301 atcgcctgcg tcacttccag agtgaccgga tcaagacgat aaaccacgcc acctttatcc
     3361 agtttgcggc tttgcgaagt ggcgagccac agcgcgtttt cttgctggct ataagccatt
     3421 tcgtaggcac ctttacctac cgctttacgc agcatttctt ctgcggcctg cgtactgaat
     3481 gatgaagcaa caagcaatga acctaacagt aatgaaccac gcaggcgcga tgaaaacaga
     3541 tgacgtaaat gcatgacgac tccctttgat gaaccgatat ttttgctgct tcacaatgct
     3601 tcgctaagcg taatcatcgt tacgctcttg ttataaatgg gaatggtaat cattattttc
     3661 tcaaatgtgg agcatttctt gccgttacga ctacaatttt ctcaattact taacgtattg
     3721 tgccgtttca ttttccataa atttacaatt tcattaccat tacggcgttt tcccattctt
     3781 aacctggctt ttttcatgaa gattttttcc gtccgacaga ccgttttgcc cgcactgctt
     3841 gtcctttccc ccgttgtttt tgccgctgat gaacagacca tgattgtcag tgccgcgccg
     3901 caggtggttt ccgagctgga tacgccagca gcagtaagcg tggtggatgg cgaggagatg
     3961 cgcctggcaa caccgcgcat taacttgtcc gaatcactga ccagcgtgcc tggtttgcag
     4021 gtacaaaacc ggcagaacta tgcgcaagat ttacagctgt cgattcgcgg atttggctcc
     4081 cgctccactt acggaattcg cggtattcgc ctgtatgtgg acggtattcc cgccaccatg
     4141 cccgacgggc aagggcaaac atccaacatc gatttaagca gtgtgcaaaa tgtggaagtg
     4201 ctgcgtggcc ccttctctgc cctgtatggc aacgcgtctg gcggtgtaat gaatatcacc
     4261 acccagaccg gacaacagcc accaaccatt gaagccagta gttactacgg cagttttggc
     4321 agctggcgct atgggctgaa agcaaccggc gcaacgggcg acggcacaca gcccggcgat
     4381 gttgattaca ccgtctcaac cacgcgtttt acgacccacg gctatcgtga ccatagtggc
     4441 gcacagaaaa atttagccaa tgccaaactg ggcgtacgca ttgatgaagc cagcaaatta
     4501 agcctgattt tcaatagtgt ggatatcaaa gcagatgacc ctggtgggct aaccaaagca
     4561 gaatggaagg cgaatccaca acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc
     4621 atcaagcaaa ctcaggctgg gttgcgctat gagcgtagcc tgagcgcgca agatgatatg
     4681 agtgtgatga tgtatgccgg agagcgagaa acgacccagt accagtcaat cccgatggcc
     4741 ccccaactta acccgtcgca tgcgggcggc gtgattaccc tgcaacgcca ttaccagggg
     4801 atagacagcc gctggacaca ccgtggtgaa ctgggcgttc cggtcacgtt cactaccggc
     4861 ctgaactacg aaaacatgag tgaaaaccgc aagggctaca ataactttcg cctgaatagc
     4921 ggcgtgccgg aatacgggca aaaaggtgag ttgcgtcgcg acgaacgcaa tctgatgtgg
     4981 aacgtcgatc cctatttaca gacgcagtgg cagctgagcg aaaaactgtc gctggatgct
     5041 ggcgtgcgct acagctccgt gtggtttgat tccaacgatc attacgttac tccgggtaac
     5101 ggcgatgaca gcggtgatgc cagttatcac aaatggctac ctgccggttc gttaaaatat
     5161 gcaatgaccg atgcctggaa tatctatctg gcagccgggc gtggttttga aacgccgacg
     5221 attaatgagc tgtcttatcg cgctgacggg caaagcggta tgaactttgg tttaaaacca
     5281 tccaccaacg atacaattga gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt
     5341 ctcgcattgt tccagaccga caccgatgat gaaattgttg tcgatagcag tagcggtggg
     5401 cgtacgactt acaaaaatgc cggaaagacc cgtcgtcaag gcgctgaact ggcatgggat
     5461 caacgtttcg cgggagattt tcgcgtaaac gcgtcctgga cctggcttga tgcgacctat
     5521 cgcagcaatg tttgcaacga acaggattgt aacggtaatc ggatgccagg gatcgcccgt
     5581 aatatgggct ttgcgtcgat aggttatgta ccggaagatg gctggtatgc aggcacggaa
     5641 gcgcgttata tgggcgatat tatggcagat gatgaaaata cggcaaaagc gccgtcttat
     5701 actctcgtcg gcttattcac cgggtataaa tacaattacc acaatttaac tgtggattta
     5761 tttggtcgtg tcgataattt attcgataaa gaatacgttg gttctgtcat tgtcaatgag
     5821 tcaaacgggc gatattacga accttcgccc ggacgaaatt atggtgtcgg catgaatatt
     5881 gcgtggagat ttgagttatt accttgatgc ccggtatttg ccgggcattt actttaacga
     5941 ttgtattgct ggtataaaat agcaatattt tgttgcataa tttcagcaag acaatgtctg
     6001 catccctctt tgtcttttgc ttttaacgca gcgagtaaca gatgatagtt ttctatatgc
     6061 tcccgtaatt ccgctggatt aatcgcttca tagagataat gtaaaccagg ccccatcctg
     6121 acccacagtt gctcaatcat ctcacacagg atgggcatat ttgagcgatg ataaatcgct
     6181 aagcgaaata gcctgtttac attaatgatt tgttccatgt cgcccttttc ttgcgcctgc
     6241 tgtaatttct cgagcaattc ctggagttcc gccaggtctt gcggggtgag gttttcaaca
     6301 gccagagcaa ctgccattaa ttccagttcg tagcggatcc gattgatttc gtctaattga
     6361 cgtttcccca cttccggaac tgtaaatgct tgtgcaggtg cgacagaaag cgcattcacc
     6421 gaaaccagac gtaataatgc ttcacgcaca ggtgtaatac tcatacctaa ttgctccgcc
     6481 agatttttag taatcaggcg tgcgccaggt tttaatgcgc caatactaag ctgatgtttc
     6541 aggtcgttct caacctgcag agtcaaactg acatgtttca tttttcccat tccaggcatt
     6601 ttaattcctt cgcgtgtttt ctgtccaacc ctgcctgcac ggcagaagga gttcacctgg
     6661 tgtattaaag tgaataatca taaaccaccc acctttgttg ttattagcag cagaacaatg
     6721 acgtggttag ttaattaaag ctgaaaataa gctttttgcc tggattttta tggcggctta
     6781 cgccgccgtt gaaactttaa tcatcacccg cgacgcggat caccactttg ccgaagtttt
     6841 tacccttcag caggccgata aacgtctgtg gcgcattctc taaaccatca gtaatatctt
     6901 cgtggtagtg gattttatcc tctttcaccc attgccccat ctccttctga aactcatgga
     6961 tgcggtgacc ataatcctga gcgataataa acccttgcaa gcgaatacgt tttttcagca
     7021 ccgttgccat caacaggggt aaacgatccg gaccgggtgg tagctctgta gcgttatagc
     7081 tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt aagtaatggt agcactgcat
     7141 caaatacctt accgcccacg ttttcataat agatatcaat accttttggg cacgctttca
     7201 ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca aacatcgaag cccagcacct
     7261 cgatagcatg gcggcatttt tccgcgccac cggcaacccc caccaccctg caacctttaa
     7321 gtttgccgat ttgccccacc gttgctccca caggacctgt cgccgcagct accaccaacg
     7381 tttcgccctc tttaggctga ccgatatcca gcagccccat ataagcggta aagcctggca
     7441 tccccagcac acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat
     7501 catcaccact ggatatgtca tagtcctgcc atccactgta gcccagcacc cagtcgccag
     7561 gctgataatc aggatgattc gactccacaa cacggctcac cgtgccgccg accatcacgc
     7621 cgccaatatc aacaggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg
     7681 ggtccaggga caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgacat
     7741 catcttcttc aagacggaaa ttctccggaa ccggtgcgcc atgtggacgc gaggccagaa
     7801 cccaacgtcg attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga
     7861 atccatcaat agtagcaggc tcacgggatt ggttttgttt gcagccgtca catcccctct
     7921 gcaacacgca ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga
     7981 taacatcatc atcattattt tatcgaggcc agacatgtcc atccgttttg cccgcaaagc
     8041 cgattgtgct gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg
     8101 gaatgaccaa acggttgatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc
     8161 aggttatccg gtgctagtaa gtgaagaaga tggcgtagtg acgggatatg cctcgtttgg
     8221 cgactggcgt agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc
     8281 cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg
     8341 ggatagcggg aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cctcgttgca
     8401 tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtgg gcactaaatt
     8461 tgggcgctgg ctggatctga catttatgca gttgcaactc gacgaacgca ctgaaccgga
     8521 cgcgattgga tgaatcagtc gctcaccctt gcttttttga ttgccgccgg aattggtctg
     8581 gtagtccaga acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc
     8641 atgttgctga actcactggt ggggattgtg ctctttgttt ctattttatg gtttaaacaa
     8701 ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacccggc
     8761 ttgctgggat cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg
     8821 acaactattg cagtactggt cgccagccag ctaattggcg gtctgatgct ggatatcttc
     8881 cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa tttgtggcgc aattttactt
     8941 gtggttggcg cctggttggt agccagacgc tcattttaat cagaggatgg tgccgccttt
     9001 ggttaactgt tcgtggcgcg cttccatatc ttctttatgc ttgcgaccgt gatgagcaat
     9061 agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc
     9121 gctcagggcg attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc
     9181 cacgcgcgcg atgacttcat ccagatgcga catactttct ccttaaccac ttgtgaaatt
     9241 tatcgattca tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt
     9301 aaaacagtga aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa
     9361 aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg
     9421 cgattgctgc cataaccgca taaaaaacaa gcatatgttg atagagagac atgtacgggc
     9481 gtccagccag atgaataaaa cgcgcgcaaa catgcgtcat tttttgacgt gcatcaagtt
     9541 ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg cccttatcca tcttcagaat
     9601 gagtattatt gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg
     9661 gcaaaaatga atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt
     9721 ttgtttactc ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc
     9781 agcacgtact gtcagagaaa tcaactctct gacagtacgt aagatcatct catactctcc
     9841 aggtttacaa cattatcctt aatatttact tcttttt
//
LOCUS       DAZFAH010000164         9663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFAH010000164 DAZFAH010000000
VERSION     DAZFAH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.165"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..1516
                     /gene="gltP"
                     /locus_tag="ACHJWB_003743"
     CDS             203..1516
                     /gene="gltP"
                     /locus_tag="ACHJWB_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4315309.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVITKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1594..2283)
                     /gene="yjcO"
                     /locus_tag="ACHJWB_003744"
     CDS             complement(1594..2283)
                     /gene="yjcO"
                     /locus_tag="ACHJWB_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4315310.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2377..4524)
                     /gene="fdhF"
                     /locus_tag="ACHJWB_003745"
     CDS             complement(2377..4524)
                     /gene="fdhF"
                     /locus_tag="ACHJWB_003745"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4105..4107),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO4315311.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRNTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSLTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4722..6188)
                     /gene="mdtP"
                     /locus_tag="ACHJWB_003746"
     CDS             complement(4722..6188)
                     /gene="mdtP"
                     /locus_tag="ACHJWB_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO4315312.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6185..8236)
                     /gene="mdtO"
                     /locus_tag="ACHJWB_003747"
     CDS             complement(6185..8236)
                     /gene="mdtO"
                     /locus_tag="ACHJWB_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO4315313.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8236..9267)
                     /gene="mdtN"
                     /locus_tag="ACHJWB_003748"
     CDS             complement(8236..9267)
                     /gene="mdtN"
                     /locus_tag="ACHJWB_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4315314.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASTMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPSTIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9286..9561)
                     /locus_tag="ACHJWB_003749"
     CDS             complement(9286..9561)
                     /locus_tag="ACHJWB_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4315315.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2169 a         2577 c         2598 g         2319 t
ORIGIN      
        1 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
       61 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
      121 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
      181 ctccattgag gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct
      241 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
      301 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
      361 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
      421 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
      481 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
      541 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
      601 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
      661 gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg
      721 gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
      781 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
      841 gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact
      901 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
      961 gcgcctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
     1021 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
     1081 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
     1141 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
     1201 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
     1261 gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg
     1321 tattccgctg gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
     1381 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattacca agtgggaaca
     1441 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
     1501 aactgcggat caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct
     1561 gatgcgacgc tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
     1621 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
     1681 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
     1741 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
     1801 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
     1861 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
     1921 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
     1981 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
     2041 cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc
     2101 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
     2161 aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
     2221 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
     2281 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
     2341 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
     2401 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc
     2461 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
     2521 gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
     2581 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
     2641 cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
     2701 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcagcgaacg
     2761 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
     2821 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc
     2881 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat tgcggcaagg
     2941 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
     3001 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
     3061 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
     3121 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
     3181 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc
     3241 ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc
     3301 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
     3361 gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc
     3421 tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
     3481 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
     3541 acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac
     3601 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
     3661 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc
     3721 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
     3781 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
     3841 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
     3901 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
     3961 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
     4021 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
     4081 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
     4141 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
     4201 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
     4261 ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg
     4321 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
     4381 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
     4441 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
     4501 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
     4561 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
     4621 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
     4681 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
     4741 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
     4801 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
     4861 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
     4921 cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
     4981 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
     5041 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
     5101 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
     5161 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc
     5221 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
     5281 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
     5341 ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc
     5401 agagattctc gtgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
     5461 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
     5521 ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
     5581 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
     5641 tctactgctg cggtttctgc tagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
     5701 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
     5761 gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
     5821 ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa
     5881 ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg
     5941 agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt
     6001 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt
     6061 ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag
     6121 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
     6181 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
     6241 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
     6301 ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
     6361 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct
     6421 gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat
     6481 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
     6541 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
     6601 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
     6661 tccgcattac tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg
     6721 cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga
     6781 tacccatggc gcgatcgcga atttccacca ggtcgtacac cgggccaaaa acgttttcga
     6841 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
     6901 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
     6961 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
     7021 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga
     7081 cgatcacgca tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg
     7141 tgtaacagat caaacaggcg agcagcgttt ttaccgcgta gcgcatatag tctggattgg
     7201 taaaagcatc ggcggccatt gatggcggtt tggctgcggg cgttggcggc gtattcgggt
     7261 ccatctgacc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
     7321 ccatcgcttc actttcactg atccgccagt cgctttgcca gcactgacct tcagcaacgg
     7381 catgctgcag cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag
     7441 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
     7501 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
     7561 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
     7621 cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcctgatgca
     7681 tttgcgaaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
     7741 gatagaggcc aacaacgata caccatagcg ttaagcgcac gaccacttcc ggatagtcga
     7801 gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacggcgaaa aagaccagtc
     7861 ccagccgatg ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgatca
     7921 atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca
     7981 gcatcgtggc gaccacaaat aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa
     8041 taccgtaaaa cagcactgcc agcgataacg ccacgaaagg gatctcaaag gtcatcgaga
     8101 tcagaatcac cagcaggcag cctacccaga gttgcacggt ctgcggcacg cggcctggtc
     8161 gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg
     8221 agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa
     8281 catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac
     8341 ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg
     8401 tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat
     8461 caggcgaatc gttgagggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt
     8521 ggcgatcacg taccagtgac gagtatcgat tagggtaaaa atagggcgca tcgtagatgc
     8581 aaactgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt
     8641 cgccatttcc agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgctgggc
     8701 aactaatgca tccacgccgc tgacggcgct ggcggctgac tgcgcctgca acaatacagc
     8761 attaagatcg gcttctgcag cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga
     8821 gacaaaacct tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc
     8881 ggcggcacgg gctttttcta ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc
     8941 aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt
     9001 cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac
     9061 tgcctggttg tcggtgaccg ccagttctac aatgcgaccg ctgacttccg gcaccacatc
     9121 aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat
     9181 aacgaaaaca agggcaacca gcgccaacgc aaccactaac agagcaggga atttactgcg
     9241 aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag
     9301 ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag
     9361 attgatattt gcccgctgaa taactcgcct cgtgatgagc gtcaaaataa ggctggcaat
     9421 ggcgcagaaa aaccagctgg gataataagc gccgatcacc gggattgccg gagataatga
     9481 gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat
     9541 gcgagagaga actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt
     9601 gaatatcaac atccattgaa ggcgtcagta tggctatttt cttgatgaat aaaaatagct
     9661 tta
//
LOCUS       DAZFAH010000165         9642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFAH010000165 DAZFAH010000000
VERSION     DAZFAH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.167"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..328)
                     /gene="cspA"
                     /locus_tag="ACHJWB_003750"
     CDS             complement(116..328)
                     /gene="cspA"
                     /locus_tag="ACHJWB_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4315316.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(609..899)
                     /locus_tag="ACHJWB_003751"
     CDS             complement(609..899)
                     /locus_tag="ACHJWB_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4315317.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1333..2043
                     /locus_tag="ACHJWB_003752"
     CDS             1333..2043
                     /locus_tag="ACHJWB_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4315318.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2093..3067)
                     /gene="ghrB"
                     /locus_tag="ACHJWB_003753"
     CDS             complement(2093..3067)
                     /gene="ghrB"
                     /locus_tag="ACHJWB_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4315319.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3171..3830)
                     /locus_tag="ACHJWB_003754"
     CDS             complement(3171..3830)
                     /locus_tag="ACHJWB_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4315320.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3983..6316
                     /gene="bisC"
                     /locus_tag="ACHJWB_003755"
     CDS             3983..6316
                     /gene="bisC"
                     /locus_tag="ACHJWB_003755"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4315321.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6285..6725)
                     /locus_tag="ACHJWB_003756"
     CDS             complement(6285..6725)
                     /locus_tag="ACHJWB_003756"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4315322.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6722..7285)
                     /gene="tag"
                     /locus_tag="ACHJWB_003757"
     CDS             complement(6722..7285)
                     /gene="tag"
                     /locus_tag="ACHJWB_003757"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4315323.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7443..8141
                     /locus_tag="ACHJWB_003758"
     CDS             7443..8141
                     /locus_tag="ACHJWB_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4315324.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8370..9572
                     /locus_tag="ACHJWB_003759"
     CDS             8370..9572
                     /locus_tag="ACHJWB_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4315325.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGTTLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2280 a         2360 c         2504 g         2498 t
ORIGIN      
        1 tcacaaaaat aatactagat tttattacgc gatcgattat ttatttcctg aaaaacaaat
       61 aaaaaaatcc ccgccaaatg gcagggatct tagattctgt gcttttaagc agagattaca
      121 ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt
      181 tctgaccttc gtccagagat ttgtaaccat cgttctggat agcagagaag tgtacgaaca
      241 cgtctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt
      301 ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt
      361 tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg
      421 tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc
      481 aaaccgttga tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta tttttcatat
      541 ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg
      601 ataaaagtct attccatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc
      661 aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg
      721 actgatacgc ccaaaacccg ggcgaaatca tcgattttta accctgtccc ttttcgtaac
      781 tgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct
      841 ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt
      901 gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt
      961 cgggaaaagc agacacagcg gggtgaagtc agggcatgct ctggctatga ttcgattcag
     1021 ccagtcattt agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt
     1081 accactcctg gtttgtaaag atttcgaaaa tctaaccttc tgcgaaggcg taaatctctg
     1141 gctacaaaaa aagcaacggc actatcattt tttaacaatg aaacaacaaa ttaaccttgt
     1201 ttcattttct ccatgatttt accaggaaga ttacgtagaa agggtatcct gcaagcctgt
     1261 cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa
     1321 aggagtaaga aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg
     1381 ttattaatgg tagttagcct gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg
     1441 tttatcgact tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc aaccctgact
     1501 gccgatcaga aaaaacagtt tggtcctttt gtctctgatt acgcgattct gtatggttat
     1561 tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga cagcgttaac
     1621 gcaattcgcg taccgcagga ttacgttacg caaagcggtc cgctgcgtga aatgaacggt
     1681 tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt tgcaggccga tgctgctcac
     1741 tctgcgctaa aacagaccga tgatttgaaa ccggtctttg atcaggcatt caccaaagtg
     1801 gtaacaacgc cagctgatgc attgcaaccg ttgattccgg ctgcacaaac tttcacgcaa
     1861 caactggtga tggttggaga ctacattgct caacaaggta ctcaggtgag ctttgtagca
     1921 aatggtatcc agttcccgac ttcgcagcag gcaagtgaat ataacaaact gattgcgccg
     1981 ttaccggcac aacatcaggc gtttaatcag gcctggacta cagcagttac tgcaacccaa
     2041 taaagagtaa aagcccgagc gattatcggg ctttacttca cgcagtcgcg gcttagtccg
     2101 cgacgtgcgg attcacacag ttcttctcaa cctttccttg taacgcatca atcaaattat
     2161 ccacggcaca ggcggccatg ccataacgcg tctcatgggt ggcagatcca atatgcggta
     2221 ctgcgacgac gttggccatt gagagcaacg gcgaatctac ggacagtggc tcttgttcga
     2281 agacatccag cccggcggcg tgaatttcgc ctttctgcaa tgcagcaatc agtgcatttt
     2341 catcaaccac cgggccacgt ccggcattaa tgaaaatggc ggaggatttc attttggcga
     2401 attgttctgc gccaaacaga tgatgcgtct catcagttaa cggcaggatc aggcaaacga
     2461 aatctgactc ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg
     2521 cttctttatg gtggcggcgc gcgttataga ggatgggcat gttgaagcca aagtgcgcac
     2581 gttgtgccag cgccataccg atccgtccca tcccgacaat gcccagtgtt ttatggtgaa
     2641 cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct tttacccgtt
     2701 ctgctacctc cacaacccga cgagcggtag acaacaccag cgccatcagc gtatcggcga
     2761 cggtttctgt taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg gtaagcgcat
     2821 cgacatcaaa attgtcatag ccgacggaga tcgttgatgt ggcacgcagt ttcggcattt
     2881 tttccagcaa tgcggcatct acattctcgt ttgaacccag taaaccttca gcttcggcaa
     2941 aaattgctgc attttgttcg acggtttgtg ggctgaggtt tgccacctgg tgaacggtga
     3001 aatgctcttg caggcgttgc agtaaatcat caggtaaggc tttgtagagg ataacggacg
     3061 gcttcatgct tctctccatt cactgataag gcgatcacct tagcctgaaa tatgatgaag
     3121 gaaaactgaa gaatgccagg tgatttctgc atcacctggc atgaaaggga ttacagcggg
     3181 cttaaggtaa tttctacacg gcggttttgc gccttacctt ctgcggtgct gttgctggcg
     3241 attgggttag ccgggccaag gccctgagta cggatgcggc tggcgtccac gccctgggtg
     3301 atcaacgcgc tggcaacgga atccgcacgt tgctgggaga gacgcatgtt caggtcgtga
     3361 ccccccgtgc tgtcggtata accaatcacg ttaaccgccg ttttcggata ctctttcagc
     3421 accattgcca cgccggtcag ggtgttagcg cctgccggtt tcagggtcgc gctgctgctg
     3481 tcgaaggtca cattgttcgg catattgagg ataatgttat ccccgctgcg ggttacgcta
     3541 acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat gtaataaccc
     3601 acgccgccgc ccagagctgc gcctgctgct gcgccaatca gcgcgccttt accgcgatct
     3661 ttcttcgaag aagagagcgc accaataccc gcgcccacga gagagcccag acctgcgccg
     3721 atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt tagttgtgca gccagatacc
     3781 gccagagcgc cactcactac ggcggcaata agataaacac gtttcttcat tgttaatcct
     3841 taataacctt tttattcttt gccacgggtt ccgtggcggg agattatgcc gcgtgaacat
     3901 gaagattatt cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc
     3961 taaccttaca ggagtcttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc
     4021 cgcccactgg gggcccatgc tggttgaaac cgacggcgaa accgtgttta gctcgcgtgg
     4081 cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag
     4141 caatacgcgg gtacgatttc caatggtgcg caaaggcttt ctcgcgtcac cggaaaaccc
     4201 gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggatct
     4261 tattcaccaa caacataaac gcattcgtga ggcttatggt ccggcatcga tttttgctgg
     4321 ttcctacggc tggcgttcaa acggcgtgct gcataaggcc tcgacattat tacaacgcta
     4381 tatggcgctg gcaggcggtt atatcgggca tctgggggat tattcgaccg gtgcggcaca
     4441 ggcgatcatg ccgtatgtcg tgggtggcag cgaagtttat caacagcaaa ccagttggcc
     4501 gctggtgctg gaacatagcg atgtcgtggt gttgtggagt gctaacccac tcaatacgct
     4561 gaaaattgcg tggaatgcat ctgatgagca ggggctttct tacttttccg cactgcgtga
     4621 cagcgggaaa aagctgatct gtattgatcc aatgcgatcg gaaaccgtcg atttctttgg
     4681 cgataaaatg gagtgggtgg caccgcacat gggcaccgat gttgcgctga tgctggggat
     4741 cgcccatacg ctggtggaaa atggttggca cgacgaagcg tttctgacgc gttgcaccac
     4801 aggttatgcc gtcttcgcct cttatttgct gggcgagagt gacggaatag cgaaaaacgc
     4861 cgaatgggcg gcagagattt gtggggttaa cgcagcgaaa atccgcgagc tggcggctct
     4921 tttccaccaa aacacgacca tgctgatggc tggctgggga atgcagcgcc aacagtttgg
     4981 tgagcaaaaa cactggatga tcgtcacgct ggcggcaatg ttggggcaaa tcggcacacc
     5041 cggcggcggt tttggtcttt cttatcattt tgccaatggt ggtaacccca cgcggcgttc
     5101 tgcggtgctc tcttccatgc agggcagctt gccgggtggc tgcgatgcgg tggataaaat
     5161 ccctgttgcc cgcattgttg aagcactgga aaaccctggt ggcgcatatc aacacaacgg
     5221 tatgaaccga catttcccgg atattcgttt tatctggtgg gcgggcggtg ccaactttac
     5281 tcatcatcag gataccaatc gtctgatccg tgcctggcaa aaaccggagc tggtggtgat
     5341 ctctgaatgc ttctggacgg cggcggcgaa acacgcggat atcgttctgc ctgcgactac
     5401 ctcttttgag cgtaatgatc tcaccatgac cggtgattac agtaatcagc atctggtgcc
     5461 gatgaagcaa gtggtgccgc cacgctatga agcgcgtaat gattttgatg tttttgccga
     5521 gttgagtgaa cgttgggaga agggcggcta tgcgcggttt acggaaggaa aaagtcagct
     5581 gcaatggctg gaaacgtttt ataacgttgc ccggcagcgc ggggcaagcc agcaggttga
     5641 attgccgcca tttgctgagt tctggcaagc caaccagtta attgagatgc cggaaaaccc
     5701 ggacagcgag cggtttattc gcttcgctga tttttgccgc gatccgctgg cgcatccgtt
     5761 aaaaaccgcc agcggcaaga ttgaaatctt ctcacagcgt attgccgatt acggttaccc
     5821 ggattgccct gggcatccaa tgtggctgga gccggacgaa tggcagggca atgccgaacc
     5881 agaacagttg caggtacttt ctgcccatcc ggcgcaccgc ctgcacagcc agctgaatta
     5941 cagttctctg cgcgaattgt acgcggtagc aaaccgtgag cctgtcacca ttcatcctga
     6001 cgatgctcag gcgcgcggca taaccgaagg ggatatggtg cgggtgtgga actcccgtgg
     6061 gcagatcctt gccggagcgg tcattagcga gggaattaaa cctggcgtga tttgcattca
     6121 cgaaggggca tggccggatc tggatttaac cgctgacggt atttgtaaaa acggcgcagt
     6181 gaacgtgctg accaaagatc tccccagctc gcggctgggg aatggctgtg cgggtaatac
     6241 ggcgctggca tggctggaaa aatacaacgg tccggaactg acacttacag cgtttgaacc
     6301 accggccagc tcataatcca ggtgggtagt tgggtttcat cctgccatgc gcaatcgaca
     6361 atgtgaaaac cctgcgcacg gtaaaaatct atcgccggtt gatttttttg ataaacctcc
     6421 agcatcagat ggggatagcg ctgctgcaca tactgcatca gcgccttacc aataccgcgt
     6481 ctgacggcct tcggtgcgac aaacattgct gccagaaatc ggccttccat aatgctgaca
     6541 aaaccgagaa gcttaccgtc ctcttcccag acccagtttt gcgcgttggc aagataggca
     6601 tcccgcacca gcggaatgca gtcacgccag taattcgctt ttataaaggg atgcccccag
     6661 gttgtacttt ccagccacag ttcgaggatc gcggggagtt ctgaacgttg cgcttcccga
     6721 atcatggttt atttcccgga tagcaacagc agccaaccac atgatcattc accagcccac
     6781 atgcctgcat aaaggagtaa cagattgtgg tgccgacaaa cttaaaacca cgttttttca
     6841 gtgccttaga tagggcgtcg gaggcggacg tagatgtggg aatttcgctc aacgttgtgg
     6901 cttgtgtcac ctgtggctga tgatttacaa acgaccagac aaagtcggca aacggttcgc
     6961 cgttctgttc catttgcagg tacgcccgcg cattaccaat aattgcctga attttccctc
     7021 gatggcggat aatcccggcg tcctgtacca gtctttcgac atcctcttcc tgcattgcgg
     7081 caaccttcac cggatcgaac tgatgaaagc aggcgcgata gttttcgcgt tttttgagga
     7141 cggtgatcca cgataaccca gcctgctgcc cttcaaggca gatcatttcg aacagttttt
     7201 tactgtcagt ttcaggcacg ccccactcat tatcatggta ggcaatataa agcgggtcct
     7261 gactcaccca gccgcaacgt tccatacttt ccctcgctat gtacccgatc gtaaaaattt
     7321 cagccgcttt tcttgacgca tcggtgaaaa agtcaatatt taatacaggg ctactatgcc
     7381 cgatatcctt catacaatga caataatatt gccgaattcg gctcttccct ggagtcgtgt
     7441 taatgatcat aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg agcgtggtaa
     7501 tcagtgcctt ttttctcaac acagcttacg cctggcaaca agaatatatc gttgatacgc
     7561 aacccggact ttccacagag cgttacacct gggatagtga tcatcaacct gattacaacg
     7621 atattttgtc gcaacgtatt caaagtagcc aaagggcgct gggactggaa gtcaatctgg
     7681 cggaagaaac tcctgtggat gtgaccagca gtatgagtat gggctggaat tttcctttat
     7741 atgaacaggt tacaaccggc ccggtcgcgg cattacatta cgatggcaca accacctcga
     7801 tgtataacga gtttggcgac agtactacca cgctgaccga tccgttatgg catgccagcg
     7861 tgagtacctt aggctggcgt gttgactccc ggcttggcga tctccgaccc tgggcgcaaa
     7921 tcagctataa ccagcaattt ggcgagaata tctggaaggc gcaatcaggc ctgagccgga
     7981 tgacggcgac aaaccagaac ggcaactggc tggatgtcac cgtaggcgct gatatgttgc
     8041 tcaatcaaaa tattgccgcc tatgctgcgc taactcaggc agaaaatacc actaataata
     8101 gcgactatct gtatacgatg ggggttagcg ccagatttta acgtaacagt cacaattgaa
     8161 accattgaat aacaatagtt gtggcgatag tgggtgctaa cttacaaaat aataaatttg
     8221 gtgaataatt gtcgcgtcat tcattcctga actaaggcat ttcattccgt tctgatggca
     8281 tttcatgccg tttttcccca ggcataaaat gcacttcgtt atggttgtcg gcagagattt
     8341 ttccttttta ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct
     8401 ggctgacact catcggtact atcattaccc agtttgcgct ggggtcggtt tatacctgga
     8461 gcctgtttaa tggcgcgctt tctgccaagc tggatgcgcc ggtaagccag gtcgctttct
     8521 ctttcggctt gttaagtctg gggctggcaa tttcgtcttc tgttgcgggc aagttgcagg
     8581 aacgttttgg cgttaaacgc gtcaccatgg cttccggcat tttgctggga ttaggcttct
     8641 tcctgaccgc gcattccaac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg
     8701 gtctggcgga tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag tggttcccgg
     8761 agcgtaaagg tctgatatcc gcgttcgcta tcggttctta tggtctgggt agcctgggtt
     8821 tcaaatttat cgacacgcag ctgctggaaa cggtgggtct ggaaaaaacc tttgtgattt
     8881 ggggagcgat tgcgctggtg atgattgttt tcggcacaac gttaatgaaa gacgcaccaa
     8941 aacaggaagt gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg gcagagtcga
     9001 tgcgtaaacc gcagtactgg atgttagcgg taatgttcct gactgcctgc atgagcggcc
     9061 tgtacgtgat tggggtagcg aaagatatcg cccaaagtct ggcacacctt gatgtggttt
     9121 ccgcagccaa tgcagtaacg gttatttcca tcgctaacct ttcaggtcgt ctggtgctgg
     9181 ggatactgtc tgacaaaatt gcccgtatcc gtgttatcac cattggtcag gtgatttcgc
     9241 tggtgggtat ggcggccctg ctgtttgcac cattaaatgc agtgacgttc tttgcagcga
     9301 ttgcctgcgt ggcatttaac tttggcggca ctattaccgt ctttccgtca ctggtcagtg
     9361 agttcttcgg cctcaataac ctggcgaaaa actacggtgt gatttacctc ggcttcggta
     9421 tcggtagcat ttttggttcg attatcgcct cactgtttgg cggtttctat gtgaccttct
     9481 acgtgatttt cgccctgctg attctgtcat tggcgctttc tacgacgatt cgtcagccag
     9541 agcagaaaat gttgcgtgag gcgcatattt agaaatatgt tgctactgaa ataaatacga
     9601 ggagggagac ttcaaagtct ccctttttta tggacgcata tt
//
LOCUS       DAZFAH010000166         9624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFAH010000166 DAZFAH010000000
VERSION     DAZFAH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.168"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1080)
                     /locus_tag="ACHJWB_003760"
                     /pseudo
     CDS             complement(1..1080)
                     /locus_tag="ACHJWB_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1087..1479)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003761"
     CDS             complement(1087..1479)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4315326.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDALTQYIQDELRSIWHGGAAFVFV
                     NKARSRIKVLRWDKHGGWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            complement(1466..1795)
                     /locus_tag="ACHJWB_003762"
     CDS             complement(1466..1795)
                     /locus_tag="ACHJWB_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4315327.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQCCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLLLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(1885..2341)
                     /locus_tag="ACHJWB_003763"
                     /pseudo
     CDS             complement(1885..2341)
                     /locus_tag="ACHJWB_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(2344..2621)
                     /locus_tag="ACHJWB_003764"
                     /pseudo
     CDS             complement(2344..2621)
                     /locus_tag="ACHJWB_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2982..3890)
                     /locus_tag="ACHJWB_003765"
     CDS             complement(2982..3890)
                     /locus_tag="ACHJWB_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4315328.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGLLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            4017..5375
                     /locus_tag="ACHJWB_003766"
     CDS             4017..5375
                     /locus_tag="ACHJWB_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4315329.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYATGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            5387..6415
                     /gene="gtdA"
                     /locus_tag="ACHJWB_003767"
     CDS             5387..6415
                     /gene="gtdA"
                     /locus_tag="ACHJWB_003767"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO4315330.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEDQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRMGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFSSRVARTTD
                     STIYHVVEGSGQVTIGNEIFTFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            6431..7132
                     /locus_tag="ACHJWB_003768"
     CDS             6431..7132
                     /locus_tag="ACHJWB_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4315331.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDKQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVIPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            7141..7785
                     /gene="maiA"
                     /locus_tag="ACHJWB_003769"
     CDS             7141..7785
                     /gene="maiA"
                     /locus_tag="ACHJWB_003769"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO4315332.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVLLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLIEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            7800..8993
                     /locus_tag="ACHJWB_003770"
     CDS             7800..8993
                     /locus_tag="ACHJWB_003770"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO4315333.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGTQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTNRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            9343..9483
                     /locus_tag="ACHJWB_003771"
     CDS             9343..9483
                     /locus_tag="ACHJWB_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315334.1"
                     /translation="MPPHKIRQEWLALDETAEVILQHSITLQSADLIRHSFIWRNRSI
                     CL"
BASE COUNT         2186 a         2505 c         2587 g         2346 t
ORIGIN      
        1 cagagtccgg taagctttat gtccgtcgac aaccagcgtc ccgccccagc cctgaagcca
       61 gttttcagga tactcatggc tacgtccggt ccggcagttg aagcacacaa ctgacggtcc
      121 cgtcctttct ccactgacgt atgcccacag ataaccggag caggatttac cgcctttctt
      181 cgtgttcagg agcttcaggg tagtctcatc tgcatgcacc accggacggt ttgcgtatta
      241 ccgtgaagga gatcggttat tgagtgatct cctttcatga aaatacgcac ggtttatcaa
      301 ctcgcgatgg agtaacgccg ccaggggaga taatgcggca cccgccgcgc caaccatgtc
      361 agccatcgaa ctgacgggaa gcccgacatc tgaacgggca aagacctgtt gctggcgata
      421 cagaggcagg tggtcaccgt atttattgat gatcacctgt gcgatgactg atggctcaac
      481 ggcacttttc gggaggatat gtgccggcat ttcaccgctg aacaccttct ggcaacaggg
      541 acaactgtat tgtggacgga cataacagtt caccacaaag tgagcgggaa tatactccag
      601 cttttcactc accgcatcac ggatatagtg cagcggctcg tcacattcag ggcaatggtc
      661 agtgtccggc tggataattt tttccacccg tggaagatga accggtaagg gtttgcgtat
      721 cgggcgtgaa cgggacgctt tttcgtcttc ttcaggggat tgcgggagca gtttatccag
      781 atgtgcggta agcgcggcga tatcggcatc aacatcctct tcgaacaggg agcgttgcat
      841 accagacagc gtttcacatt ttttaccgaa gcgctgttga cgaaccagtt tcagcatttc
      901 ttccagaagc tggatgcgcc ggcttttctc cagtaattcc cgctcttttt cggcattctc
      961 cgacatgact ttttgtacca tggtaagtgc catagcacgc agtttttcga tgtcattcgt
     1021 ggtgttgaga gcggagatat ccatacaggg gagtatacca ctccagatac ggatattcat
     1081 tatttttcac tgagttagca gtgtattttg cgtgttttca ggacgcagtt caggttcatt
     1141 ctgccagacc catttcgtca ggtcatgtcc cttaacctgc tgccagtcaa caccggcaat
     1201 cagccagtta aactcgtcgg gagttaggtg ccaggcagcg tcatttgcac gtggccagcg
     1261 gaagctgcct ttgtgcagac ggcgggtaca caaccacccc ccgtgtttat cccaccggag
     1321 aactttgata cgcgaacggg ctttgttaac aaagacgaag gcggctcccc catgccatat
     1381 cgaccggagt tcatcctgta tatactgcgt cagtgcgtcg atgccccggc gcatgtcaac
     1441 aggtttaatt gcgatgaaga cattatcagg actgagcata tttgagcgcc ctgaagacat
     1501 cggtaagttg tgacggctga cagcacatcc gtactccacc gggaaggagg agcgtgactg
     1561 gctcattcgt gacgggctgt ggtacatcag catgcagtgg tggcgcaata tgaacgggaa
     1621 gaacggtcgg ttcatttgcc cggcgttcag cttttgcgac atcctgcggc cattcgcgaa
     1681 gtgatgtgaa gggaatatcg ttgagttcac agcattgctg acgtgtaaga ccactggcgc
     1741 gccaggcagc aacgtgctgc cttttctgtt caatggtcca tcggggtttt gacatcgatt
     1801 atctccggta agttggtgaa tgaatgtcgg taatactaac tgactgaaga tccctgtgaa
     1861 gatgacttcc ccgtaacgac acagaatatc ttccgcaata cgctgactac cagaccagaa
     1921 cagaccgtcc agtgcggtca ccagtaatcc ctcctctagc gcatacttaa gtcattcacg
     1981 gagcgctttc actttccggg caatctgctg ccaaggcgat gatgtatttc attcccaacc
     2041 atccggcgaa tacggtggga cagatgatac cggaatgtat ccgcacgccg gaaagactgg
     2101 ccttcaggct atacacgcaa ccaaagcgtt tatcgttaaa caccacattt ttctggctga
     2161 tatcccattc ttcccaaagc acagcaatca gctgtgacgt gcgggttaaa agcagacgaa
     2221 aaggcagttc aaaactggtg gtataatcca cattcagcag ggcattacgc agccgctctc
     2281 ctgatccgac ctctgcctgc cgacactcct cctgaacatc ctgccactgc tggcgaaggc
     2341 gtacaacaac acgaacattc tcagcgtatt tttttgagta accaactgaa aaatggtggg
     2401 aagatcattt ttcagttgag aattccccaa cggtcaatcc acccgcttta tcttgttttt
     2461 aactaatgct gtctggttaa atgacgcccg atgctgctaa gcgtgaggga agcgccattt
     2521 attacagcat ttgttgcgtc aatcagtgat ttttgatggt agaaatgcaa agtggccagt
     2581 atgtttttaa ggaatttttg gcataatgaa cgtgcaggca tagtggtgat atccttgaaa
     2641 ttgctagcac aatcaattag atcacatctc actatgcctg tcttgttttc tgggaattcc
     2701 taatgggttg tccttccaat tggttctttc gaggcctgct cagattcact cacattatga
     2761 cctgtatact cactgaaaca cctacagctt cttcatcaaa tacatcagcc gctttgttac
     2821 ccacacgact gctttgactg ctaccggctg gaacgacagt cacctggtgg gattttcacc
     2881 ccatgaaaga tcacgcctta tcacagtgca cgattccccg aaaaattacg catcgttgaa
     2941 ttacagggaa gtaacatcgt gcgaatgccc agttcagcac actaattctg ctgtaaaaag
     3001 tgcagaaact gacttaaagc ataagaaggc gtttcgttat tgcgccagaa cacaccgacg
     3061 ctgcctttct gttctatctt tggcaggttg agtatcacca gtagtccctg gctggcataa
     3121 cgttgcgcca ggcgtaatga caaaatagag atcatatcgc tgctttgtaa caggttggta
     3181 ctgacgttca tcgacgccga ctcaatcgtg ttttctggca gcattacgcc gtggtctacc
     3241 agcgcattat caatactaat gcgaatgggg gtacccgtcg gccagacgat ccagcgccag
     3301 ttggcaagat cattccagct gagggtggcg tatttcgcca gtgggtgctc agggcgggcg
     3361 acaaagcata ctggctcggt gtacagcacc tgataattca gtggcagttg caaggcgcga
     3421 ccgccaattc gcccaacgac cacgtcgaca gaacctgcca acagatcttg cagcaatggg
     3481 gtcatcacct tctcttcgat attcaggtgt agagttggca tcttcgtaag caaatgcaaa
     3541 atcgcctggg atacgcagtc agtggcgacg ggagaacagc caattttcag actgccgacc
     3601 agtccacctt gtttaaaacg ggccagttca tgttgcgatc gctccatgtc attgattaac
     3661 cgttgggcat gttgcaataa caatttgccg ccttcagtcg gacgtaatcc tttgctgtgc
     3721 cgctcaaaaa gtgtgattcc aatctcatct tcgagttgtg acaaccattt cgaaagcgca
     3781 ggttgggtga tattcatcat tcgtgcgaca tgggtcagat tgccctgttc acccagcgcc
     3841 accagcattt caaggtgatg tagttttaat ttttgcgccc agttcgccat ttaccataac
     3901 ctccaggtta tctctctgcc cgaaatgcca ttgtacattt catctcttta atcacaatat
     3961 cgcaacatat acaaaacaaa agaaacatct accttacctg cactgaggca tttactatga
     4021 ctcaacgacg tgatctacaa gcacttatcg atgctgctcc ggtcggcaag atgcagtggc
     4081 gcgttatcat ctgctgtttt ctggtggtaa tgctcgatgg ttttgacacc gcggctatcg
     4141 gatttatcgc ccctgatatt cgcactcact ggcaactcac cgcaggcgat ctcgccccgc
     4201 tttttggcgc gggtctgctg gggttaaccg caggcgcact gctttgtggc ccgttgtccg
     4261 atcgctttgg tcgcaagcgg gtgattgagc tgtgcgtctt tttgtttggc gcgttaagcc
     4321 tcgcctcagc tttctctccg gatctgcaaa ctctggtctt cctgcgcttt ctcaccggcc
     4381 tgggtctggg cggcgcgatg ccgaacacca tcaccatgac ctcggagtat ctgcctgccc
     4441 gccgccgcgg cgcgctggtg acgctgatgt tttgcggctt tacgctgggt tcggcgtttg
     4501 gcgggattgt cagtgcgcag ctggtgccgg tgattggctg gcacggcatt ctggtgttgg
     4561 ggggcgtgtt accgctgatg ctgtttgtcg cactgctggt agttctgccg gaatcgccac
     4621 gctggcaggt gcgccgccag ctaccgcagg cggtgattgc gaaaaccgtc agcgccatca
     4681 cccgcgagcg ttatgtcgat acccattttt atctcattga atcagcctcc gttacaaaag
     4741 gcagcattcg tcaactgttt atgggacgtc agttacccat cacgttgatg ctgtgggtag
     4801 tgttctttat gagcctgctg attatttacc tgctctcaag ctggatgccg acgctactga
     4861 accatcgcgg catcgacctg caacacgcgt cctgggtcac cgccgcgttc cagattggtg
     4921 gtacgttagg cgcgctggcg ctgggtgtgc tgatggataa gttcaatcct ttccgcgtac
     4981 tggcgctgag ctatgccaca ggcgctatat gcatcgtgat gattggctta agccaggatg
     5041 gattatggct gatggcgctg gcgattttcg gcactggcat tggcattagc ggatcgcagg
     5101 tggggctgaa tgcgctgacc gcaacacttt atccaacaca aagtcgcgct acgggcgtga
     5161 gctggtcaaa tgctatcggc cgctgcggcg cgattgttgg ttcgctgtct ggcggcgtga
     5221 tgatggcgat gaatttctct ttcgacaccc tgtttttcat tatcgctgtg cctgcggcga
     5281 tcagtgcggt gatgttaacc ctgctgatta ccgtggttcg ccagtcgacc tctgtccctg
     5341 actcactgcc tcgcgcaggc gttgtgaacg aataaggagc atcccaatga ctgataacaa
     5401 tcaaaatagc cgcgagcaat tttatcagca tatttccggg caaaacctga ccccgctgtg
     5461 ggagtcactg caccatctgg tgccgaaaac gcccaacgct aactgtgcgc cagcgtactg
     5521 gaattatcag gagatccgcc cactgctgct ggagagcggc agcctgattg gcgcgaaaga
     5581 ggcggtgcgc cgcgtgctgg tactggaaaa cccggcgctg cgagggcagt cttccatcac
     5641 cgcgacttta tatgccggtt tgcaactgat catgccgggc gaagtggcac cgagtcatcg
     5701 tcataaccag tcggcgctgc gttttattgt tgaaggcaaa ggggcattta ccgccgttga
     5761 cggcgaacgc acgccaatga atgagggcga ttttatcctg accccgcagt ggcgctggca
     5821 cgatcacggc aaccctggcg acgaaccggt tatctggctc gatgggttgg atctgccatt
     5881 agtgaacact ctgggctgcg gttttgccga agattacccg gaagatcaac aaccggtaac
     5941 gcgtaaagag ggcgattacc tgccgcgtta cgctgccaat atgttgccgc tgcgccatca
     6001 gacggggaac tcctcgccga tctttaacta tcgttatgac cgcagccgcg aagtgctgca
     6061 cgatttaacc cgcatgggcg atgccgacga atgggatggc tacaaaatgc gctacgtcaa
     6121 cccggtcacc ggcggctatc cgatgccgtc gatgggcgct ttcctgcaac tgctaccgaa
     6181 agggttctcc tcccgcgtgg cgcgcaccac tgacagcact atctaccatg tagtggaagg
     6241 tagcggacaa gtcaccatcg gcaatgaaat cttcaccttt agtgcaaaag atatctttgt
     6301 ggtgccgacc tggcacggcg tgtcattcca gactacgcaa gataccgttt tattcagttt
     6361 ttcggacaga ccggtacaag aagccctggg gcttttccgc gaagcgcgtt attaatttgg
     6421 gagagagaaa atgactcaat atgtattcga accacaagcg cccgttaccg taccggttgt
     6481 tggcagtgat aaacaattcc ccgtgcgtcg cgtttactgc gtcgggcgta actatgccgc
     6541 ccacgcccgc gaaatgggct ttgatccgga tcgtgaaccc cccttcttct tctgcaaacc
     6601 agcagatgcc gttattccgg tcgccgctgg cgaaacgctg gcgctgccat atccggcgca
     6661 gaccgataac tatcattacg aaattgaact ggtagtggcg attggtaaaa aaggcagcga
     6721 tatcccgctg gaacacgccc atgaatatgt ctggggttat gccacgggtc ttgatatgac
     6781 tcggcgtgac cgtcaaatgg aaatgcgcca gatgggccgc ccgtgggaaa tcggtaaagc
     6841 gtttgatctc tcagcgccca tcgcgccgct gcacaaagcc cgcgatatcg gcggtattga
     6901 taatgcgcca atctggttac aggttaacgg cgaagatcat cagcgcagcg atattcgcca
     6961 tctcatctgg tcggtgaatg aaaccatcag ctatctgtca ggcttctttg agctgcaacc
     7021 gggcgatctg atcttcaccg gtacgccgga aggggttggc ccggtagtga aaggtgatgt
     7081 gatcacaggc aacgtcgagg gcttaacgcc tatcgcggtg aagattgtct gaggtgcatg
     7141 atgaagctgt acagtttttt taatagttca gcgtcgtacc gggtacgcat tgcgctggcg
     7201 ctgaaaggca ttaattacca gaccgaaggg gtgaatatcc gtattggtca gcaaaatgaa
     7261 ctggcctatc ggcggatgaa tccggtcggg ctggtgccga cgctgctgac tgacgagggc
     7321 gaatcactcg gacaatcgct ggcgattatt gactggctgg agaggcacta cccgcaagtt
     7381 ctgctggtgc cgcaggaaga accggcccgc aacaaggtgc tggaaattgt ctatgccatc
     7441 gcttgtgata ttcatccatt gaataacttg cgcgtgctgc gctacctgat cgaagaactg
     7501 aacgtcagtg aagaagaaaa aaaacgttgg tatgcgcact ggatccagca agggttaagc
     7561 gcggtggaac aactgttgcg ccagaaccag tcggggcaat tttgtgtggg cgagacgccg
     7621 acgctggcag attgctgcct tgtcccgcag tgggcaaacg cgctacgcat gaactgcgat
     7681 ttgagcgggt atccacgctg caaagcggtg tacgacgcct gcacacagct gccagcgttc
     7741 attgccgcag cgccagaaaa tcaacaagat aaaatttcag cctgaccagg agaactataa
     7801 tgacgaaagt gacacgcgca gtaattgtgg gaggcgggat cggcggtgcg gcaactgcgc
     7861 tgtcactagc ccgcctgggg atcaaagtgg tgctgctgga aaaggcgcat gagatcggtg
     7921 agattggcgc gggcatccag ttggggccaa atgcgttttc ggcgctggac agcctcggcg
     7981 tcggcgaagt tgcccgccag cgcgcggtgt ttaccgatca cattaccatg atggatgcgg
     8041 tgaacgccga agaagtggtg cgcattgaaa ccgggcaggc ctttcgtgac catttcggcg
     8101 gtccttacgc ggtgatccac cgggttgata ttcacgcctc ggtgtgggaa gcggtactga
     8161 cgcatccgga cgtggaatat cacacctcca ccaacgtcgt ggatattcgc gagacggaag
     8221 acgatgtcac cgtgtttgac gatcgcggca atagctggac cggcgatatt ctgattggct
     8281 gtgacggcgt gaagtcagtg gtgcgccaga gcttactggg cgatgcgccg cgtgtcaccg
     8341 gacatgttgt ctaccgtgcg gttatcgatt gtgccgatat gccggaagat ttgcgcatta
     8401 acgcaccggt actgtgggcg gggccgcact gtcacctggt gcattaccct ttgcgcggcg
     8461 gcacgcagta taacctggtg gtgacattcc acagccgcca gcaggaagag tggggcgtga
     8521 aggatggcag taaagaggaa gtgctctctt actttgaggg gattcacccg cgtccgcgcc
     8581 agatgctgga taaaccgact tcatggcgac gctggtcaac tgccgaccgt gaaccagtcg
     8641 cgaaatgggg cactaaccgc attacgctgg tgggcgatgc agcccatcca gtggcgcaat
     8701 atatggcgca aggtgcctgt atggcgctgg aagacgcggt aacgctgggt aaggcgctgg
     8761 cgcagtgtga gggtgatgcg gcgcaagcct ttgcgctgta tgagtcggta cgtattccgc
     8821 gcaccgcgcg tattgtctgg tcgacccgtg agatgggacg ggtttatcac gccgcgggag
     8881 tagaacgcca ggttcgtaac ctgctgtgga aagggaaatc gcaggcagag ttttatcgcg
     8941 gcatggagtg gctgtacggc tggaaagaag ataactgtct gcaaccacgc tgagtacttc
     9001 ttcacctaac caggcgcacg ggtgatctcc ttgcccttcg gagaggagct attaagtcct
     9061 gttgatttca acagggctct ctcctctccc tttagcctcc tttaaaataa ggtatcatca
     9121 gaaattcttc tttcaacaaa tagcacgcca cttcaagcaa atcgtctcca gagctattca
     9181 gtgttacgtc ctgtttttcc agtgtaaatc atcaccagat tatgtctgaa aagctcgtta
     9241 cctacactga tcagtgttag ttgttttggc aaaaatatct cacaccggtt ttcatcatca
     9301 acaagcacta tacgcaacca acaacacaaa aaggaacaca ccatgccccc acacaaaatc
     9361 cgccaggagt ggctggcgct ggacgagacc gcagaagtca tcctacagca cagcatcacg
     9421 ttgcaatccg ccgaccttat ccgccattct tttatttggc gcaaccgcag tatctgtctg
     9481 tgatgttggt gttactgcca agcgctttat ggttcgggat aacggcatgt atggtagcaa
     9541 gtagctttaa gcgatggtta taagttgctc aaaaaatcgg tcctcactac aatacgaatt
     9601 ttatcaggta tggaattcgg atta
//
LOCUS       DAZFAH010000167         9483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFAH010000167 DAZFAH010000000
VERSION     DAZFAH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.169"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..470)
                     /locus_tag="ACHJWB_003772"
     CDS             complement(<1..470)
                     /locus_tag="ACHJWB_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HFO4315335.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQAL"
     gene            573..1343
                     /gene="yqeC"
                     /locus_tag="ACHJWB_003773"
     CDS             573..1343
                     /gene="yqeC"
                     /locus_tag="ACHJWB_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4315336.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1391..3016
                     /gene="yqeB"
                     /locus_tag="ACHJWB_003774"
     CDS             1391..3016
                     /gene="yqeB"
                     /locus_tag="ACHJWB_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4315337.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVCADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSPA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3057..3989)
                     /gene="arcC"
                     /locus_tag="ACHJWB_003775"
     CDS             complement(3057..3989)
                     /gene="arcC"
                     /locus_tag="ACHJWB_003775"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4315338.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4037..5422)
                     /gene="hyuA"
                     /locus_tag="ACHJWB_003776"
     CDS             complement(4037..5422)
                     /gene="hyuA"
                     /locus_tag="ACHJWB_003776"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4315339.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5475..6686)
                     /locus_tag="ACHJWB_003777"
     CDS             complement(5475..6686)
                     /locus_tag="ACHJWB_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4315340.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6744..7940)
                     /gene="dpaL"
                     /locus_tag="ACHJWB_003778"
     CDS             complement(6744..7940)
                     /gene="dpaL"
                     /locus_tag="ACHJWB_003778"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4315341.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(7998..9185)
                     /gene="ygeW"
                     /locus_tag="ACHJWB_003779"
     CDS             complement(7998..9185)
                     /gene="ygeW"
                     /locus_tag="ACHJWB_003779"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4315342.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2202 a         2320 c         2499 g         2462 t
ORIGIN      
        1 agtgcctgac gcatattggt gacattgggt cgttggattg cctgtatcaa gcatggtttt
       61 gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt
      121 cgtaacgacc agatttttct aaaaatatcg atggtgaggg ttggcatgtc accgtgggtg
      181 agaaaacaat gttctgtttg cactgcgggt actgcggcct tgacggatgt tagtaaaccc
      241 tgcgcataat ctgggttgtg aataatagtg atattgctct ggttcgcata acgttcgtgc
      301 agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt
      361 ttgatacttg tatcaagaat tgttccctgt tcccagggta acatcatttt ccattgcccc
      421 attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca
      481 ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg
      541 gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat
      601 tagtcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg
      661 ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt
      721 taatcacgac gacgacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc
      781 gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca
      841 gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg
      901 tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt ggaatgccgt
      961 taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg
     1021 tgatgggagg acatactttg ggcgcgaaag tgagcacaga aaatgtccat cgctggtcgc
     1081 agtttgctga tattaccggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg
     1141 cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct
     1201 ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag ctccttcaac
     1261 cgctgcaaca acacgacgta gaggcaatct ggctgggcga tatacaagaa catcctgcaa
     1321 tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat
     1381 tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt
     1441 tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg cacaaatgtt
     1501 agtgtgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt
     1561 gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc atggacgtat
     1621 ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat
     1681 cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc
     1741 gatagcccag agtgccgcgc tattaggatt tgatattgcc gttgccgata tttatcgcga
     1801 aagcctcaat cctgaactat tcccaccatc aaccacgctt ctccatgctg agtcgtttgg
     1861 tgcggcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa
     1921 tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct
     1981 ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga
     2041 agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca
     2101 ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg
     2161 gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg
     2221 agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct
     2281 ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga
     2341 tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagccctg cggaagcgat
     2401 gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga
     2461 tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacgcg
     2521 ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag ggaaggattg
     2581 tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt actctggttg
     2641 tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat
     2701 ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga
     2761 gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt
     2821 gcgtggcttg ttgaacgacg gcctggcagt ggtcggtggt tttaaaattg gtgatatcga
     2881 tcctcgtggt gaaacggctg atttcaccag cgtttctgat aaagcccggg cgattggcgg
     2941 cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt
     3001 gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac
     3061 tgacaaatca ccgttccggt tttgccttca atcccctctt tcgctttgct caatacggta
     3121 atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc
     3181 agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac
     3241 aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca
     3301 gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct
     3361 ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac aggaatacca
     3421 ccaccgccaa cggtgatcac cacctggcct gcatctacca gagctttaac cgtttctttt
     3481 tcaataatat caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct
     3541 tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa
     3601 ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta
     3661 accagcgttg ctaccggttt attaatgccc cgagaaagca gttcttctct taaggcgttt
     3721 tgtagatcgt aaccaatata accctggctt aaggcaacgc agacagacat cggcagcatt
     3781 ggcgagtgcg cttcagtttt tgcagcagct tcaaaggcct ggttaatcat gcccacctgc
     3841 ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc aacaattgcc
     3901 tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg
     3961 cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat
     4021 agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac
     4081 ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg
     4141 ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt
     4201 ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat
     4261 cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg
     4321 cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa
     4381 acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc
     4441 ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt
     4501 caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg
     4561 gtgggctaag aatgaacttc atgccgtctt ctgtatcgta actgcgttcg tccaacagga
     4621 gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat
     4681 aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt
     4741 gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg
     4801 catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag
     4861 ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga cggcgtaatg
     4921 cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc
     4981 tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta atgtgctgga
     5041 tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct
     5101 ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg
     5161 ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga
     5221 tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa acgtaacagc
     5281 cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa
     5341 caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc
     5401 cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa
     5461 taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc gtacatcgcg
     5521 gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct
     5581 ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta
     5641 gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc
     5701 accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg
     5761 gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg
     5821 ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg
     5881 ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt
     5941 ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa
     6001 cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg
     6061 ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt
     6121 tccatacgac cgcgctgacc acggtatacc tggcagtcgg ttggttcggt actgaccaca
     6181 aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct
     6241 tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta
     6301 atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga
     6361 ccaccgatca gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg
     6421 ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg
     6481 atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc gactttttcc
     6541 atctcttctt taatacgatg tactacgcgt ttctcgtcgc agctttcact gggaatagca
     6601 accatgtcgc gcaggaagcg agtcatatcc gcctggtaat cttttgcttt ttcaagaatc
     6661 agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc
     6721 agggagcagt tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact
     6781 tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac cacggcatct
     6841 ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg
     6901 agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac
     6961 ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg
     7021 gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggcct
     7081 gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga
     7141 taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctatag
     7201 acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga
     7261 acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt
     7321 gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt
     7381 accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag
     7441 ttcatatctg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga
     7501 gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgcgc tgcccacgcc
     7561 acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca
     7621 tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat
     7681 tgagcgatag cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa tcgttttgat
     7741 tcgtctttga caagaatttt cttcacccca aaaaggtttg cgagatcgtc cagcgcacaa
     7801 agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg
     7861 ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc
     7921 ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag
     7981 ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc
     8041 agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc
     8101 cagctggctt ctttgtaggt agcgatacgg tatttttcga atacgccttc agtcacttcc
     8161 ccttctttgc aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg
     8221 ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc
     8281 tgtgccagac actgtttttc cagtgctttt aagccttcgt gatcgttagc gcgcagcaat
     8341 tcggtacgct gttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgca
     8401 tctttgaacg cttcttccat gctggtgacc tgacggaagc taccgccaga tgctttagca
     8461 ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg atgggccagg
     8521 gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt
     8581 ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc
     8641 agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt cgggtggtca
     8701 atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg
     8761 tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag atacatatcg
     8821 tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt
     8881 tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag gccgagcagg
     8941 ttaagcgcgg aagcataaga gaagcgggta cgggtggagt tgtcgcggaa tacggaaata
     9001 cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc ttttaatgct
     9061 gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt taacaaaaaa
     9121 tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc attaacagtt
     9181 ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca ctgtgacttg
     9241 ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt gccagaatta
     9301 tccgcgcggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc acaaattgat
     9361 gccagcttct ggttgtttgt aaaatacgaa aacgtttggc tttttttatc gcgttattta
     9421 tttgctgcta agtatcagaa ataagataaa ggggcaggct atctctcttc agtttgataa
     9481 act
//
LOCUS       DAZFAH010000168         9225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFAH010000168 DAZFAH010000000
VERSION     DAZFAH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.170"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..405
                     /gene="drpB"
                     /locus_tag="ACHJWB_003780"
     CDS             124..405
                     /gene="drpB"
                     /locus_tag="ACHJWB_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO4315343.1"
                     /translation="MEERLSRSPGGKLALWAFYTRCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            445..1140
                     /locus_tag="ACHJWB_003781"
     CDS             445..1140
                     /locus_tag="ACHJWB_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO4315344.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLVADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLCEEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDGKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1207..2625
                     /gene="dcm"
                     /locus_tag="ACHJWB_003782"
     CDS             1207..2625
                     /gene="dcm"
                     /locus_tag="ACHJWB_003782"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4315345.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRCSR"
     gene            2606..3076
                     /gene="vsr"
                     /locus_tag="ACHJWB_003783"
     CDS             2606..3076
                     /gene="vsr"
                     /locus_tag="ACHJWB_003783"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4315346.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3065..3985)
                     /gene="yedA"
                     /locus_tag="ACHJWB_003784"
     CDS             complement(3065..3985)
                     /gene="yedA"
                     /locus_tag="ACHJWB_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4315347.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4158..5075
                     /locus_tag="ACHJWB_003785"
     CDS             4158..5075
                     /locus_tag="ACHJWB_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4315348.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISEFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKAFSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5154..5336
                     /locus_tag="ACHJWB_003786"
     CDS             5154..5336
                     /locus_tag="ACHJWB_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4315349.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5525..7201
                     /gene="dgcQ"
                     /locus_tag="ACHJWB_003787"
                     /pseudo
     CDS             5525..7201
                     /gene="dgcQ"
                     /locus_tag="ACHJWB_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506077.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(7198..8013)
                     /locus_tag="ACHJWB_003788"
     CDS             complement(7198..8013)
                     /locus_tag="ACHJWB_003788"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4315350.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8312..8539)
                     /gene="yodD"
                     /locus_tag="ACHJWB_003789"
     CDS             complement(8312..8539)
                     /gene="yodD"
                     /locus_tag="ACHJWB_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4315351.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8702..8890
                     /gene="dsrB"
                     /locus_tag="ACHJWB_003790"
     CDS             8702..8890
                     /gene="dsrB"
                     /locus_tag="ACHJWB_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4315352.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(8934..>9225)
                     /gene="rcsA"
                     /locus_tag="ACHJWB_003791"
     CDS             complement(8934..>9225)
                     /gene="rcsA"
                     /locus_tag="ACHJWB_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4315353.1"
                     /translation="SLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGT
                     IQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2252 a         2230 c         2500 g         2243 t
ORIGIN      
        1 tgttcaaaaa atgattgagt cttgactggc tcattcaatg tggaaaaatg tgacttttat
       61 cacataatag tactaagtct gaattttccg ggttatctca aaatggaata cggttcgaca
      121 aagatggaag agagactctc tcgcagccct ggcggcaaac tggctttgtg ggctttctat
      181 acacggtgtg gctatttcgt ctgggcaatg gcgcgttaca tctgggtgat gagccggatc
      241 cccgatgccc ccgtgtcggg atttgaaagc gatttaggct ctaccgcggg aaagtggtta
      301 ggggcactgg tcggattttt atttatggct ctggtcgggg cattgttagg cagcattgcc
      361 tggtacaccc gacctcgtcc cgcacgtagc agacgctatg aataaaaact gctaaggtgg
      421 cgactctttg gcaacggagc gcgtatggac ttacaacact ggcaggcaca gttcgaaaac
      481 tggttgaaaa accatcatca gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc
      541 gtctgggcta cggcacagaa gctggtggca gatgacgatg tcgatatgct ggtgatttta
      601 accgcctgtt attttcacga tattgtgagc ctggcgaaaa atcatccgca gcggcagcgt
      661 tcttcaatcc tggcggcaga agagacgcgt cgcctgctct gtgaagagtt cgagcaattt
      721 ccggcggaga aaattgaggc cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa
      781 atcgccccct taacaacgga agctaaaatc gtccaggatg cggatcggct ggaagccctg
      841 ggggcgattg gcctggcgcg cgtgtttgcc gtttcagggg cattgggcgt ggcgctgttt
      901 gatgccgaag atccgctcgc acagcatcgc ccgcttgatg gtaaacgcta cgcgctggat
      961 catttccaga ctaagttgtt gaagctaccg caaaccatgc aaaccgtaag aggcaagcag
     1021 ctggcgcagc acaacgcgca atttttagtc gagtttatgg cgaagctcag tgccgaactg
     1081 gcgggggaga atgaaggtgt cgatcacaag gtgatagatg cgttttcacc cgcgggctaa
     1141 gcgcgtggcc ctaaatggct gtaattatgt taacctgtcg gccatctcag atggccggtt
     1201 aaatctatgc aggaaaatat atcagtaacc gattcataca gcaccgggaa tgccgcacag
     1261 gcaatgctgg agaaactgct gcaaatttat gatgttaaaa cgctggtggc tcagcttaat
     1321 ggtgtgggtg agaatcactg gagcgcggca attttaaaac gtgcgctggc gaatgactcg
     1381 gcatggcacc gtttaagtga gaaagagttc gcccatctgc aaacgttgtt acccaaacca
     1441 ccggcacatc atccgcatta tgcgtttcgc tttatcgatc tatttgccgg aattggcggc
     1501 atccgtcgcg gttttgaatc gattggcgga cagtgcgtgt ttaccagcga atggaacaaa
     1561 catgcggtac gcacttataa agccaatcat tattgcgacc cggcgacgca tcattttaat
     1621 gaagatatcc gcgacatcac cctcagccat aaagaaggcg tgagtgatga ggcggcggcg
     1681 gaacatattc gtcaacacat tcctgaacac gatgttttac tggccggttt cccttgtcag
     1741 ccattttcgc tggctggcgt atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc
     1801 tgcgatactc agggcacgct gttctttgat gtggtgcgca ttatcgacgc gcgtcgtccg
     1861 gcgatgtttg tgctcgaaaa cgtcaaaaac ctgaaaagcc acgaccaggg taaaacgttc
     1921 cgcatcatca tgcagacgct ggacgaactg ggctatgacg tggctgatgc agaagataac
     1981 gggccggacg atccgaaaat catcgatggt aaacattttc tgccgcagca ccgtgaacgc
     2041 atcgtactgg tgggttttcg tcgcgatctt aacctgaaag ccgattttac tctgcgtgat
     2101 atcagcggat gtttccctgc gcagcgagtg acgctggcgc agctgctgga cccgatggtc
     2161 gaggcgaaat atatcctgac gccggtactg tggaagtacc tctatcgata tgcgaaaaaa
     2221 catcaggcgc gcggtaacgg cttcggttat ggaatggttt atccgaacaa tccgcaaagc
     2281 gtcacccgta cgctgtctgc gcgttattac aaagatggcg cggaaatttt aatcgatcgc
     2341 ggatgggata tggccacggg tgaaaaagac tttgacgatc cgctgaatca gcaacatcgt
     2401 ccacgtcggt taacgcctcg ggaatgcgcg cgcttaatgg gttttgaagc gccgggagaa
     2461 gcgaaattcc gcattccggt ttcggacact caggcctatc gccagttcgg taactcggtg
     2521 gtcgtgccgg tctttgccgc ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg
     2581 gcgttgcgtc agcaagaggc acaacatggc cgatgttcac gataaggcca cacgcagcaa
     2641 aaatatgcgc gcgattgcca cgcgtgatac ggcgattgag aagcgcctcg ccagtttgtt
     2701 aaccgggcag ggactggcgt ttcgcgttca ggacgccagt ctgcccggac gcccggattt
     2761 tgtcgttgat gaatatcgct gcgtgatatt tacccatggc tgcttctggc atcatcatca
     2821 ctgctatctg tttaaagtgc ctgcgactcg aaccgagttc tggctggaga agataggtaa
     2881 aaatgttgag cgcgatcgcc gcgatatcag tcgcttgcag gaactcggct ggcgcgtatt
     2941 gattgtctgg gagtgcgcgt tacgtgggcg cgagaagctg acggatgaag cgcttagcga
     3001 gcgtctggaa gagtggatct gcggcgaagg tgccagcgcg cagatcgaca cgcaggggat
     3061 tcatttactc gcttgatgcg tcctgaataa ctggcgcaac tacgggtttt gccggaaaga
     3121 gatattttcc caacgtgacc agcaccaccg cgaagacaat tacgccgagc gccagccatt
     3181 caatcttcga aagcgtctct ccacccagtc ccgtacccag caagaccgcg accaccgggt
     3241 taacgtaggc gtagctggtg gcgagagccg gactgacgtt gcggattaaa tacatataag
     3301 cgttgatggc gataatcgaa ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa
     3361 gggaagggag cgccgtcagt ttttcacccg caatcatcga cgcgatcatt aacaccacgc
     3421 ctgccgccag catctcaatc gcacccgcca tcatccctac aggtaaggta atgcgcgagc
     3481 cataaactga gccaaacgcc cagctaatcg agccgattaa aatcagaatc gcgccccacg
     3541 gattgccgct taaatttcca ccgctattga gcatgatgat tccggcaagc ccaatggcaa
     3601 tacccaccca ttccagtttg cgcgttttaa tgccaaacag gcggctgaag cacagggtaa
     3661 agaggggcac ggttgcaacc actacggcgg cgatgccgga aggaacattt tgatgttcgg
     3721 caaccgtcac catgccatta ccgacagcca gcaataacag gccaatcagc gcggcattga
     3781 gcagcggacg tagcgggggg agcttgtgtc cgcgcagtag caaaaatgcc agcaataaaa
     3841 taccggctgc caggaatcga acgcccgcca tcattaatgg aggccagctt tccacgccaa
     3901 tccgaatgac aaaataggtt gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa
     3961 gcggtaataa ctggcggaaa cgcataatcc ctcacggtgg aaataaaaag gtggttcata
     4021 gtaaacgtga aaatcattct gctggcgaga gatataattg cacttgattg ttaaaaaaat
     4081 gttgacctgt gaagcaagta tcagagctgc cgtttttgct tcatacttac acccttcaac
     4141 aataaaaatg agagggaatg cttttggccg ggagtagttt actgacgttg ctcgatgata
     4201 tcgccacact gctggacgat atctccgtga tgggcaaact ggcggcgaag aaaaccgccg
     4261 gtgtattagg ggatgactta tcgctcaatg cgcaacaagt ttcaggtgtg cgggccaacc
     4321 gggaacttcc cgtggtctgg ggcgtggcga aaggatcgct gatcaataaa gtgattctgg
     4381 tgccactggc gctgattatc agtgagttta tcccgtgggc gattacgcca ttgttgatga
     4441 tcggtggcgc gtttctctgc tttgaagggg tagagaaagt gctgcatatg ctggaggcgc
     4501 gtaaacataa agaagatccg gcgcagagtc agcagcgtct ggagaagctg gcggcgcagg
     4561 atccgctgaa gtttgaaaag gacaaaataa aaggggcgat tcgtaccgat tttatattgt
     4621 ctgcggaaat cgtcgccatc acgctgggga ttgtggccga agcgccgttg cttaatcagg
     4681 tgctggtgct ttcaggcatc gcgctggtag tgaccgtggg cgtctacggt ctggtagggg
     4741 ttatcgttaa gattgatgac ctggggtatt ggctggcgga aaaatccagc gcgctgatgc
     4801 aggcattagg taaaggatta ttgattatcg cgccctggct gatgaaagcg ttttcgattg
     4861 tcggcacgct ggcgatgttc ctcgtcggcg gcgggattgt ggtacatggt attgcgccgc
     4921 tgcatcacgc cattgaacat ttcgccgggc agcaaagtgc agtggtggcg atgatattac
     4981 cgactgtttt aaatctgatt cttggattta tcatcggcgg catcgtggtg ctgggagtga
     5041 aagccgtagc aaaaatgcgc ggtcaggcac attaatactc gcacaagcta ttatgcaaaa
     5101 ttcgccatct tcaactaagg tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct
     5161 ttatggttag tgaggaagtt acggttaaag agggcggccc gcggatgatt gtcactggat
     5221 actccagcgg tatggttgag tgtcgttggt atgatggtta cggggtcaag cgggaagctt
     5281 ttcatgaaac cgagcttgtt ccaggggagg ggagtcgttc tgcggaagaa gtttgaaagg
     5341 gtatcattaa gaccaggccg gatcacatcc ggcctggtgt gccaatgcct gcaatgaatg
     5401 atagtcgcgt cttatcatgc caccattata aaattatcgc tgttttgcgg tacgctatcg
     5461 ccaggttatg ccagaatcat aaaaaagcag gtggggagtc gtcagggggc agcacgagac
     5521 aaaaatggaa aaccagagct ggttgaaaaa actcgcacgc cgcctggggc ctggtcatat
     5581 cgttaatctc tgctttatag tggtattgct tttttccacc ttgctcacct ggcgtgaagt
     5641 agtggtgctg gaagatgcct atatctccag ccagcgtaat catctggaaa acgtagccaa
     5701 cgcgctcgat aagcatttgc agtataacgt cgacaaactg atctttttgc gtaatggcat
     5761 gcgcgaagct ctcatagcgc cactggattt cacctctctg cgtgatgctg taaccgagtt
     5821 cgaacagcat cgcgacgagc acgcctggaa aatcgaactc aaccgacggc gcaccctgcc
     5881 agtcaatggc gtgtcggatg cattagtcag cgaggggaat ctcctgtctc gcgaaaatga
     5941 aagcctcgac aatgaaatta ccgctgcact ggaagttggt tacttgctgc gactagcgca
     6001 caactcctcg tcgatggttg aacaggcgat gtatgtctcg cgtgccggat tttacgtttc
     6061 gacgcagccg accttgttta cgcgcaatgt tccaacgcgt tattacggct atgtcaccca
     6121 accctggttt attggccatt cacaacgaga aaatcgtcac cgcgcggtac gctggttcac
     6181 ttcgcaaccg gaacacgcca gcaatactga accgcaggtt accgtcagtg ttccggtaga
     6241 cagtaataac tactggtatg gcgtgctggg gatgagtatt cccgtgcgta ctatgcagca
     6301 atttttaaga aacgccatcg ataaaaacct cgatggtgag tatcagctct atgacagtaa
     6361 gctgagattt ttgacctctt ccaatcctga ccatccaaca gggaatattt ttgatcctcg
     6421 tgaactggcc ttgctggcgt aggcaatgga acatgacacg cggggcggca ttcgtatgga
     6481 cagtcgctat gttagctggg aacgtctgga ccatttcgac ggtgtgctgg tgcgcgtcca
     6541 tacgttaagc gaaggcgtgc gcggcgattt cggcagtatc agcattgcat taaccctgct
     6601 gtgggcgctc tttaccacca tgttactcat ctcctggtat gtgattcgcc ggatggtcag
     6661 caacatgtat gttctgcaaa gttcgttgca gtggcaggcg tggcacgaca ccttaacccg
     6721 tttatataat catggcgcac tgttcgaaaa agcccgtccg ctcgctaaat tgtgtcagac
     6781 gcaccaacat cctttttctg tcattcaggt cgatcttgac cattttaaag cgattaatga
     6841 ccgctttggt catcaggcgg gcgaccgtgt tctttctcat gctgccggat taattagcag
     6901 ttccttgcgt gcgcaggacg ttgccgggcg ggtcggtggt gaggagtttt gtgtgattct
     6961 gccaggcgcg agtctgacgg aggctgcgga agtcgcagaa cgtattcgcc tgaagttaaa
     7021 tgaaaaagag atgttgattg ctaagagtac gacgatacgc atcagtgcct cgttgggggt
     7081 aagtagcagc gaggaaaccg gtgattatga ttttgaacaa ttccagtcac tggccgaccg
     7141 tcggctttat ctcgctaaac aggctgggcg taatcgggta ttcgcgagcg ataacgctta
     7201 acgggcggag aaaaaatggt ccagcccttc acgccatcct tccggtcctt cacgctgcgt
     7261 tcgccagacg cgggccggat cctcatcatg cagatgcacc ccttcacggt ttagcccttt
     7321 cacaatcacc gcgtaatcca ttacctccag taagggcgca tcgtttggcc catcgcccag
     7381 gccaagtgtg gttgggcgtt tgcctgacaa ttgttgatag gtcgcgataa tccagttggc
     7441 agcctgatct tttccggcag aggcatccag gacgtgccag aagcgcgcac cttgcataaa
     7501 ctgtaagccc agttcgttca gacgagcggt aaattgtgcc atacgctcgt cactgtcgcg
     7561 ccagattagc gttaccgacg cctcatgaag ctgcgtcagc gccgcctggc tacggcttaa
     7621 tcccgtccat tcggcgatag ttgcatcgtc gacatcatca aaagtcgtga atttaaaatg
     7681 ttctttctcg cgtagcgtat ttaaaacctg gctgatttcg ccatggctaa tacctgagat
     7741 gatgcgtgga aaaccgtcta tctcctgcca ttgctcagca agctggatca ctgcgccatt
     7801 ttcggcaatc agcggtaaac cttgtagccc caacgttttt tgcaagtaca gcatttccgc
     7861 tgatgtttta ctgctacaga gaatgacggg aacatttgct tcgcgtaaac gggtgagcca
     7921 gggggctgcc ggttgccagt cataactatg actgtccagc agggtgccat caagatcgct
     7981 aaaaaccagt agtggttgtt gaattgaaaa catagtcgcg cagtactccc cttaccagga
     8041 tgattctcaa aacagcacaa attatcaaag atgattttgt tcgggagcga tcccgctgac
     8101 gccagagcca ccaaggggtg acgtgcgtca catttctatt cataagtagc gttaatcatt
     8161 catatagcga atattttctt gtcagcgaaa aaaattgcgg ataaggtgat gaacacatca
     8221 gatttcctgg tgtaacgaat tttttaagtg cttcttgctt aagcaagttt catcccgacc
     8281 ccctcagggt cgggattctt ttattgtgca ttcaacgatt cacttcagag acgctgaagt
     8341 catgaatatc cagttcgaag gcatccgcca attcacgcca tgtatgatat tcacgtccat
     8401 cgacgctaac gtgttcagaa tcgttctggc tgcgatgaac ttcgctttcg ctaatttcat
     8461 tgatcgccgc cagcagggca tcgacatcga cgctgacctc acgttttgcg gtatcgctgt
     8521 actcttttgc ggttttcatc atctttcctc gcaaccgtta ctctgttgtt gccgccagtc
     8581 gttaagtccg cctggcatca cttaagtata gaccgtcgag aaaatcagca ctcgcgcgcg
     8641 cctggcgctg ccagaaaaac gaaattgttc tacactggca caaaggcaca ggaggaaaac
     8701 gatgaaggtg aatgatcggg taacagtcaa aacggatggc ggtccgcgtc gtcctggcgt
     8761 ggtactggca gttgaggagt ttagtgaagg cacaatgtac ctggtttcgc tggaagacta
     8821 cccgctcggc atctggtttt ttaatgaagc agggcatcag gacggtatct ttgtggagaa
     8881 agcagagtaa tcttattcag cctgactggt gggaaaccac cagtcagaat gtgttagcgc
     8941 atgttgacaa aaataccatt agtcacatta tccgtcagtc ggacgacatg gtagataacc
     9001 tgtttattat gcgttttgat cttacgttta atattacctt tatgcgatga aacggtcttg
     9061 gctttgatat tcatttggtc agagatttga atggttccct gacctgccat ccacattcgc
     9121 aacatactcg attcggttcg gctcaatgat aacgtcggca tatttaaaaa cgaggttatc
     9181 gttgtctctt ttttcagaat atcgccaagg atatcgtcga gagat
//
LOCUS       DAZFAH010000169         9112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFAH010000169 DAZFAH010000000
VERSION     DAZFAH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.171"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..788
                     /locus_tag="ACHJWB_003792"
     CDS             204..788
                     /locus_tag="ACHJWB_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4315354.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(813..1250)
                     /locus_tag="ACHJWB_003793"
                     /pseudo
     CDS             complement(813..1250)
                     /locus_tag="ACHJWB_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            complement(1693..2454)
                     /gene="envY"
                     /locus_tag="ACHJWB_003794"
     CDS             complement(1693..2454)
                     /gene="envY"
                     /locus_tag="ACHJWB_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4315355.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(2637..3527)
                     /gene="ybcH"
                     /locus_tag="ACHJWB_003795"
     CDS             complement(2637..3527)
                     /gene="ybcH"
                     /locus_tag="ACHJWB_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4315356.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(3528..6500)
                     /gene="nfrA"
                     /locus_tag="ACHJWB_003796"
     CDS             complement(3528..6500)
                     /gene="nfrA"
                     /locus_tag="ACHJWB_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO4315357.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(6487..8724)
                     /gene="nrfB"
                     /locus_tag="ACHJWB_003797"
     CDS             complement(6487..8724)
                     /gene="nrfB"
                     /locus_tag="ACHJWB_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4315358.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(8993..>9112)
                     /locus_tag="ACHJWB_003798"
     CDS             complement(8993..>9112)
                     /locus_tag="ACHJWB_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO4315359.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         2216 a         2278 c         2323 g         2295 t
ORIGIN      
        1 acctctatgt agctaggttt ttgtaaagaa actgtgcgtc actgtatcca ggactgttat
       61 gtgccaaata aggaagttga agttaccagc tcttactcta aagctattac gtatagaaaa
      121 aatgcatttg agacgtgtaa aatcatttat ttaatcgcaa tttatgtaat ggcataatca
      181 ggtcggtgaa agaggggaga agaatggata aaagagctaa aaatcagata gtcgatagcg
      241 atattgcacg tttgttactc aagctcagaa agtctcgaaa cttgactgtt acagagcttg
      301 cccaacgatc tggtgtcagt caggcgatga tcagcaaagt tgaacgaggc acttcaagcc
      361 ccagtgcaac aatacttagt cggttagcta atgcactgaa tattacgcta tcaaagcttt
      421 ttgcagagct tgagatgcaa cagaattcac ttgtactttt ggctgaccag caacaacatt
      481 ggattgatga agaaacaggc attacccgct ggtctttgtc cccagcgggt gcatgtccgg
      541 aactaataaa agttgaaatc ccccctatgg ggcaattgac tatccctgcc tctgcaaatg
      601 agcatctttc tggacaaact ctatggatgt taagcggcag ccttgatttt cgagtcaatc
      661 atcagactca tcatttacaa gccggtgatt gtcttgctct tacctttgcc agcgagtacc
      721 aaatgagaaa cccagatagc ggccagagtt gttcttacat tgtcgctttc agccaaaaaa
      781 atccatgatg taattctcct aaaattatca tgtcacattg ccttataaag atgtaataga
      841 gggtagggtc tgccttgatt gtcccgctcg gaacgtcctg aaagtttaaa gcccatatgt
      901 tgataaaagc ctacagcttg atgattttat tcatttacat caacgcttag atcaggatgt
      961 agagccagcg catgtgagat cagatgtttc ccgacgccaa gtccgcgagc tgacgcatca
     1021 acaaaaagag cctctaaatg tccctcatgc aaaaacatga atcctagtgg ctgatcttcc
     1081 tggtttgttg ctaccagcac aggggtttct gaaaaaaagc tgacaacttc tttttcaatc
     1141 tcctgccggt catgggctgt aagaaaatcg tgtgtggcat caacggaatt tttccaaatt
     1201 tggataattt ctggtgcttc agatggtcgc gacattctga tttttaacat gttatctccc
     1261 ttaaagtggt cgttgcttgt gtcttgagat acaaaacgag ttatcaaatt tactatagtg
     1321 aaattaaatt cacaatagtg atttggttcc tgtgccttaa cattacccat gaggagtaca
     1381 attaaacgga ttaatcagta tctccatttg aaagaaaaaa gacttctgta ctgtttgata
     1441 tcaccgctat tttcttattt gctgtgcagg tcgtagtggg acaaaactga gacacataag
     1501 gcctcacaat ggcttgcaag gctttacatg ttttgaggta gtgggacgtg tgagcgcaga
     1561 gatggcgtgg taagttgttg acttaaaatg tcgttctagg aacttctaag tcgtgggccg
     1621 caggttcgaa tcctgcaggg cgcgccattt cttcttcatt tatgcccgtc ttatccgttt
     1681 ccgctttgcc cttcaccaca tcactttttg tcgctgtttg gcgagataat tcaacggtgt
     1741 caggccgtaa aacgccttaa aaacagagat aaagtacgac gtgctgctat agccacataa
     1801 ttgcgccacc tgagtgatat ttttgttatc catcaataac atctgtacgg cgtaacgcat
     1861 acgacactct gtgacaatct ggctatagct ggtattttcg ttttttaatt tctttttgag
     1921 taggctgggg cttaaacata gcgaactggc gacaattcgc agattccagt aatgctgaat
     1981 atcgctttga ataatgcggc agacgctgtc gcggacgctg ctgcgtaaga tatacatcag
     2041 tagggctata aaccgcgatt gctcaagaaa gttagacaat acggtaaaaa gtaatgcgcg
     2101 cgtcaactcc gcttcgcagg gtttgctggg ttgctgcttg ctgtggttgg cggctagccg
     2161 gaacacttcc ggtgtacggc tatgacaagc gataaccggg gttgccagcc gcgaccaggg
     2221 ctttacacag gttaagttct tatttaaaaa caacagataa tcgttgatga tgttacggtt
     2281 gatgtggggg attaatacgt gatttaacgt tgagagttca atgacgttat tgttgcacgc
     2341 gaaggcggcc aggtagtttt tcggaagggt aaacgtagca tggttattga cgcttacctc
     2401 tacctctttt tcggtcagga tcaccacgca aggttcactg ctgctcaatt gcatttcgca
     2461 ctcctcagat atcagaaact ccgctcaaag gatctatgct tcctgcatga gtgatcggcc
     2521 cgttcgccga taacgatctt ctttctttag cacgcttttt agcaattaat cttgatggaa
     2581 ttctgacgag agcgaaagag gtaagccagg tcgtacccga cttacctgga ggagatttaa
     2641 tactcgagaa tgccgtgcgc gacgggcaaa tagcgcagag agaaatagag agtgtcttta
     2701 ccgcaagagg aacgtttcgc taacagcgag gcgatctctg catccggttt cggtttcgct
     2761 gtaaaggttt tgcctttgcc ggcgacaaac agttcataaa caatgccgct ggcaggggcg
     2821 ggactttggc aatcggcgct ggcctgtaga taacgttcac gctgttcagc ggtaagttta
     2881 tccacgccgc tgccatcctg tacccagaca ttaacgccgg ttgctttaac ttgttccagc
     2941 agttggcgat agccatcggg cgacatgttt ccggcgaaaa aactactgat ataaaccggt
     3001 tttgctgaaa catcgctaat cagccgctgc gcgttgttta accatgttag caaaggctga
     3061 cgggcagctt cgctgcgcca gttcaggtcg tcaatttccg cgctgatgta ccagccatcc
     3121 ggcgttacgc caggcgcggc gctccataat ctggcttgct ggagatcggc agccagcagg
     3181 cgattaagat agctttccag cgctgcggac gactgtttct ggtgcataaa aaattccgga
     3241 tcggcgttca gcccgacaat cagcttcagg ccagcctgtt gcgcagctgc ggcacgctta
     3301 aacaataacg cgcgctgttc tggctgggtg aatgcatcgc cgtaacgggt ccattgcaaa
     3361 accagggtat cgaagccttg caaacgtaac tgactcatca gcccctgcca ctgggtatcg
     3421 gtaacctgac tatctcggtt ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa
     3481 gggctgacca aaagcagtgt cagcaatacg aaaatgaact tacgcattta ccagtgcact
     3541 ccaatggtga gaaacgcgtt gttgcgctct ccgttacgtt gattaatcgc cttaaaggta
     3601 tgttgatact cgacgccgag actgactttg tgcggccagg cgtcgtagtg cgtctcgccg
     3661 gtccagatat tccagcggac cccgactccg ccaagctgcg cgccctgagt gcctttatca
     3721 cgatagccgt tgtcctgaac gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc
     3781 tgatgccagc tgacgcgata atctgccgtc cacgcctgaa tatcctggcg gatatattgc
     3841 gccgcatcga ggtacaggtt ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg
     3901 ctgtatttgc cgccattaaa gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca
     3961 ttttggccgt tcagcggcaa ctgctgttcg acggcgagga aaaagatctg atcgcgcagc
     4021 ggcttccagc gcagaccggt gccggacatc ggatttttca ccggcatcat caccccgttt
     4081 tctccggtat cggcaaagac gcggctataa actgagagca ggtcgccttc cagcagcata
     4141 ttgcgtccga tgcggtactc ggcttccagt tgtccgtagc tacgatagct tttccctggc
     4201 gctgcgccgc cgacattatt gttagcggta ctcattgcgc cggaacgcaa gccgatggaa
     4261 gaatcgaaac tgaacgtcca gcggcgaccg acctcctcat gcaaacggcg gaaattgaag
     4321 cgttgctgat tttgctctgg ggtcagtggg gttatcagcg cctgattatc aatgtcatca
     4381 atcaccagcc gggcgtagtg ctgcgtcgca ggcatgtcat ccagacgctg gttcacatag
     4441 gccagttgtc ggatcagtgc cggatcgtcc ggtagccctt tatgcgcctg ttcgagcatt
     4501 tcccgcgact gtgcgatatc accgctatcc cacaaggcgt aaccaagcgc tgcctggatg
     4561 ttgctattat tcggttccag ttccagcgcg gcgcgcaaat cactcaccgc cgccgggaca
     4621 ttatgacgtt ggcgataaat tgtcgcccgc gcaacgtaag cgttggcaga aggtgcaata
     4681 ttgattgagc gcgtgagatc gctcagtgcg agttccggct gaccaggaat gtaacgttgc
     4741 gcatgcagcc accagtagag ggcattgttt cccagtccac gttgttctgc ctgttgtagc
     4801 cagcgatcgc gagctgcacc atttcctgcc gcctgggcgg tattggcagc agcaagcaga
     4861 tcctcattgc tcatgtcgtg aagactgatt ttctgccagg ccgccagtgc ggtggcgtag
     4921 tcctcaacct gatacgcctg ataggctacc gcacgatgtt gccaggcgtt cggttgtcgt
     4981 tgttcggcct gaagccatgc atacagcgcc acaccgggta gcgtgtcccg ataacacttt
     5041 gccagacggt tccaggcggc agcatcgtag gaaggcgaca tatcgcccag caagcgaact
     5101 attgccgggc aattatctgc aatacccggc aactgacttt gccactgacg ttgctccgcc
     5161 agcggtaagg gtttcgataa aatcgccacc ttcgccggcg ttgccaggta aggatgactt
     5221 tccagcagag acgccagtcg cgccattaaa gtctggctga cacgcgcatc gccctggaaa
     5281 ggatagcgtt gcagcaataa atcggcagct tcgcgtgact gctcgttctg catcagttgc
     5341 caggtcagtt gatccaggcg ggtaagattt gccggttctt gctgatacag caatcgtgcc
     5401 agacgcagag attcagcctt gttacgggtc gccacgctga cagcataacg ctcctcaagc
     5461 atttcattgg cggggagggt ggcgagcagt ttttgcgctg cgtcgtactg accttctttt
     5521 aacagcaccg gtagcgtcgc gccaacaaca tactggcggt tatcggcaaa ctgcaccgta
     5581 taattcgcca atgcctgaac ggggttggcg ctgtatttag ataacagata gagccaactt
     5641 ttctcttgtg cgtccgtggt aaacagcggc ttattttcaa tgagataatg ctggaggcgt
     5701 gctttttcgc cacgataagc cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg
     5761 aagatcccct gtgattgcag tgccaggatc cgatcgtcca gctgcccggc aagaagcacg
     5821 tcaaaccact gacggcgttc tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat
     5881 agcgtatctg cctgggacca ttgtttcagg tagattgccc gttgcagcag atcggttcgc
     5941 agcgtttttc cttccggcga tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca
     6001 ggtaactgtg ccagccgcag ggcattctgc ccgacttcac tgcgacaacg cagggtcggc
     6061 gcagcatcgc acgctttttg ctgggcaagc agttcttcaa ctgtcgtaac gcttttcact
     6121 tcaaccggaa tagccgccag actgcgctca agtcgggcat ctcctgggtg acgtttcagt
     6181 tgatcctcaa gcaacagccg cgcccggtca tcatgaccaa aatggcgata ggcttccgca
     6241 aggtataaag tcagcggaat attatccggc acctgctggt gtatatattc aaattcgcgg
     6301 atggcggttg cttcgtcgtt atttttctgt gccttcagcg ccttatcgag acggggataa
     6361 ataacaaaat ggcgataatc gctcagcccc agctcttctg cgctggtgcc aatattgtct
     6421 gcgagtgcgc tggtactcaa taaagacgtc aacagtaaac cagaccatcc gatgacgcga
     6481 ttaaggttat tctccttcat tttcggactc cagttgcgca acctgttctg tgtttaaacc
     6541 tgctttgagt aatagtgatt gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg
     6601 atccaacgtt tcctggctga taacgccttc ggtgaccaaa aacttgccga gcggcagaga
     6661 actgcgttca tggcgcaata acaacacgtt aattgctgaa cgattaatat gaccgagcgt
     6721 ggtcagtatt tcggcgaaca ggaactgatg cggcacatat tgccgccaga tttcaccggt
     6781 ctgctgttcc gtgagccact gatgctgaac cgcattgtac aacattgccc gcggatcgtg
     6841 accgcgtcgg cgtgcatacc agtgacgtaa ccctgtgaca atttgtcccc gcagaacaat
     6901 gacgtaacgc actttgcgtc cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat
     6961 accatcttca ctgccgacaa ttaactcatc attttccaga cgcagcggca gtaccgcata
     7021 atgcagcgcc acggaggccg gcatttcggc aatcagcgag gacgggatct gccaggcatc
     7081 gatggattcc cacgccacgc cgttttgctc tgccagcgcc tgtgccagct gctcggcgct
     7141 aatcagcccc tgcatcagca ttgaaccgcc caggcgtaga ccttcgacgc gattacgcag
     7201 tgctgtatcg agttgttctt cagtgatgac ctgattttcc agcagaattt gacctaacgg
     7261 gcgcaacgag cgggtatcgc cagtgacgct ggggaagtca tgcgttgttt tatcccacgc
     7321 cacgcgacgc ggatcgccgt gctgaagaac ctgtttcagc gcgcgccagt tagccataaa
     7381 gttaatcagg ttgccccaga aaagacgcag gacggaaagc aggccctgcg tcaggccgta
     7441 gtagccagta acgaaaatca cccgctgcac gatgcggtta accatcaaac caaagtttag
     7501 ccacagcagg gtcattaacc atgcgctgcc gctgaaaata gaaaggaaat gccaggcatc
     7561 gggccacaaa ctttcatacg ccagcaacag caaaagctgg atcatcacca gcatcgcgag
     7621 gaagctgaca aagttactga ttgccccttt gcggtcgcgc cagagaaagt agttcagcgt
     7681 caggctggag gtccatttat gggttttaaa gccctggaaa acaatgccga tgatccagcg
     7741 ggatttttgt cgaaccgcag tcgaaaaggt atccgggaaa tattcgcgca cgcagatcat
     7801 gtttgacgtc cgtgcgtgct gtaaaaattt acgctgctcg cgttctttgg cttcgtccac
     7861 caccggaaaa cggacaaaaa tttccgtcat acctttttct ttcaggcgga agccaatgtc
     7921 gtagtcttca gtcagactct gcacgtcgaa agcaataccg tcaccgtcag ctaacagagc
     7981 ggtcacggcg cggcggctga aacaggtgcc gacgcctgcg ctgggcactt gtccggcgag
     8041 ggcttcacgc accggaacat ctttgccatg cagctctgaa aactcatcaa tgtaagtcat
     8101 gctggtgaag tgcgtccatt cgcgttcaaa cggatacacc gggatctgaa tcagatcttt
     8161 acgctcgacc aggtagttga acagacgcaa ttccatcggt gaaatcacat cttcggcgtc
     8221 atgcagaata aaaccagcaa aagcgaaatt ggcgctacgt tcaaactggg tgatggcgtc
     8281 cagcacgttg ttcagacagt cggctttgct ggtggggcca ggacgcgcgc agactacctt
     8341 atgcacattc gggaagcgag cgcacacttc gtcaacatca cgctgagtat cagggtcgtt
     8401 ggggtaggtg ccaacaaaga tatgatagtt ttcgtagtcg agcgtggtcg ccgccagctc
     8461 ggccatattg ccgatgacgc ccgtttcatt ccacgccgga accataatcg ctaacggttt
     8521 ttcatctggt ttatacagtt cgcggtaact cattcgcggg tagcggcgat aaacactcaa
     8581 cttgcgttta atgcggcgta cccagtagac gacatcaata aaaaaatcgt ccagcccgct
     8641 gatgaacatg atgaccgcta acgttatcgc gatgactttt aagccgtaga gccaggttgc
     8701 aaaaacatca agaagccagt ccacacaaaa accttacatt aacgctggtt atgtttaggg
     8761 tggcgtatat taaggttttt tatgaattgt gacagctttt taccattaat aggtatgact
     8821 attgcggcac gttattttta cactggttat aaaagttgcc gtttgcgtag aatgtagaca
     8881 aaagcatggt gtcaaggagg ggaggttaac gtcatagtga aaaggagcgg tggcaccgag
     8941 caggtcatca tgtagtggaa gtgcctggag gaggtgagca ggcagggcaa gattacgaaa
     9001 gcccgctccc cgcaaggact gacgcgagat agtttctgtc catggctgct tttcgcatct
     9061 tacgtcttaa ccctgccttg aataccttat cattcgttaa aatattaata gc
//
LOCUS       DAZFAH010000170         9085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFAH010000170 DAZFAH010000000
VERSION     DAZFAH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.172"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..738
                     /gene="csgD"
                     /locus_tag="ACHJWB_003799"
     CDS             88..738
                     /gene="csgD"
                     /locus_tag="ACHJWB_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4315360.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            743..1132
                     /gene="csgE"
                     /locus_tag="ACHJWB_003800"
     CDS             743..1132
                     /gene="csgE"
                     /locus_tag="ACHJWB_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4315361.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1157..1573
                     /gene="csgF"
                     /locus_tag="ACHJWB_003801"
     CDS             1157..1573
                     /gene="csgF"
                     /locus_tag="ACHJWB_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4315362.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1600..2433
                     /gene="csgG"
                     /locus_tag="ACHJWB_003802"
     CDS             1600..2433
                     /gene="csgG"
                     /locus_tag="ACHJWB_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4315363.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2497..2988)
                     /gene="ycdZ"
                     /locus_tag="ACHJWB_003803"
     CDS             complement(2497..2988)
                     /gene="ycdZ"
                     /locus_tag="ACHJWB_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4315364.1"
                     /translation="MNILLSIAITTGIFSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3090..3644)
                     /gene="ycdY"
                     /locus_tag="ACHJWB_003804"
     CDS             complement(3090..3644)
                     /gene="ycdY"
                     /locus_tag="ACHJWB_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4315365.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3668..4405)
                     /gene="ycdX"
                     /locus_tag="ACHJWB_003805"
     CDS             complement(3668..4405)
                     /gene="ycdX"
                     /locus_tag="ACHJWB_003805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4315366.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4460..5398)
                     /gene="ghrA"
                     /locus_tag="ACHJWB_003806"
     CDS             complement(4460..5398)
                     /gene="ghrA"
                     /locus_tag="ACHJWB_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4315367.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTCKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5632..5719
                     /locus_tag="ACHJWB_003807"
     tRNA            5632..5719
                     /locus_tag="ACHJWB_003807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5666..5668,aa:Ser,seq:gga)
     gene            complement(5869..6711)
                     /locus_tag="ACHJWB_003808"
     CDS             complement(5869..6711)
                     /locus_tag="ACHJWB_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4315368.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6796..6993)
                     /locus_tag="ACHJWB_003809"
     CDS             complement(6796..6993)
                     /locus_tag="ACHJWB_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4315369.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7005..7496)
                     /locus_tag="ACHJWB_003810"
     CDS             complement(7005..7496)
                     /locus_tag="ACHJWB_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4315370.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7493..7867)
                     /locus_tag="ACHJWB_003811"
     CDS             complement(7493..7867)
                     /locus_tag="ACHJWB_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4315371.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7914..8288)
                     /locus_tag="ACHJWB_003812"
     CDS             complement(7914..8288)
                     /locus_tag="ACHJWB_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4315372.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8451..8672)
                     /locus_tag="ACHJWB_003813"
     CDS             complement(8451..8672)
                     /locus_tag="ACHJWB_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4315373.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8735..>9085)
                     /gene="radC"
                     /locus_tag="ACHJWB_003814"
     CDS             complement(8735..>9085)
                     /gene="radC"
                     /locus_tag="ACHJWB_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4315374.1"
                     /translation="AREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEV
                     HPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIV
                     GGSQVFSFAEHGLL"
BASE COUNT         2323 a         2254 c         2230 g         2278 t
ORIGIN      
        1 cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg tcaggtgtgc
       61 gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat
      121 acattattgt tgatcactaa accttctttg caggcgacgg ctctcttgca gcaccttaaa
      181 caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga cgatatatct
      241 tcaggctcta ttattcttct ggatatgatg gaagcagata aaaagcttat ccattattgg
      301 caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa
      361 gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg
      421 gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga atgctacttt
      481 acgcaaaagc ttgccagcta cttgattacg cattcaggta actatcgtta taacagcacg
      541 gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg
      601 tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt
      661 tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac
      721 gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt
      781 tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgctaactga
      841 ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag
      901 tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat
      961 cacaataacg gtcaatcagg atgttatttt ccagactttt ttatttccgt tgaaaagaga
     1021 cttcgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca
     1081 gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct aaataaaaaa
     1141 ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc
     1201 attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc
     1261 aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag
     1321 ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat
     1381 ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat
     1441 ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt
     1501 gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc
     1561 aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg
     1621 gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg
     1681 acattaatgc ctcgagctca gagctacaaa gatttgaccc atctgccagc gccgacgggt
     1741 aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg
     1801 gcaagtaact tctccactgc tgttccgcaa agcgccacgg caatgctggt cacggcattg
     1861 aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct gcttaacgag
     1921 cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg
     1981 ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg ttatgaaagc
     2041 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
     2101 cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc
     2161 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
     2221 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
     2281 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
     2341 gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaacgacat tctggtgaaa
     2401 taccgccata tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc
     2461 ggcattttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg
     2521 ctcacggtgc gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
     2581 gtaaccaaat atcagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
     2641 ggcaaacgtc gcgcatgcgc ctataaacgt tcccggaaca aatgaaagca gcagctgttt
     2701 ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag
     2761 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
     2821 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa agtaggccgt
     2881 acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat
     2941 accggagaaa atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat
     3001 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     3061 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     3121 cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     3181 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     3241 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca
     3301 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     3361 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     3421 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca
     3481 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     3541 ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     3601 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     3661 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     3721 tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga
     3781 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     3841 atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac
     3901 tgcccttacg tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg
     3961 cggctgcttc agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta
     4021 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     4081 catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     4141 ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca
     4201 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     4261 gcggagcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     4321 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     4381 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     4441 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     4501 cccctttttc gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg
     4561 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     4621 gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca
     4681 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     4741 cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     4801 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     4861 cggcaaagct ttgcacgcca ggccacgatt tacaggttcg actccagcaa cgcagcggaa
     4921 agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc
     4981 cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     5041 ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct
     5101 gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     5161 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca
     5221 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat
     5281 cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc
     5341 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     5401 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     5461 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     5521 cacattgatt gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     5581 cgtgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     5641 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     5701 aatccccccc tcaccgccag attataagcg catgttattg tttaacatgc gcttttttat
     5761 tttcccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     5821 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     5881 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     5941 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     6001 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     6061 gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     6121 gtcagcctgt tgtccggtga taagatgaaa tccccaccgg tcccatctca ccagattgtt
     6181 gacgataatt ttactgccaa atttacaggg gcaattggtt ttgtaattcc agttcagccc
     6241 tctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt gcagttgttc
     6301 aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa ttatcgtact ccagtgaccg
     6361 gtaccatgta tcacgggcct gcgtatccat taatgacagc atcccggaac gttgcatcag
     6421 gtcacgccag atttcgcgat caagattccg ggtaatggct ttcattgctg tttcgggttt
     6481 ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg gggcagtttt
     6541 acctccgatg ctttccgtca gcaccgagat ttcagccagt tgtcggataa gtgtgtcgat
     6601 ttgcaacagt gtactctttc gtacagccag aatgtggcga atgctggttg agcagataac
     6661 gtccgtatgc ccgttgacca cttccggacg ggagattgtg atatctgaca taatcgatat
     6721 tcctttttgc agataaatga aaacgccagc cctgtgcagg ctggcgtcag aggaatgaag
     6781 tctggtgagt gagcttcacc ggttctggtg cagaagctgc agatgacgca gggtacggac
     6841 atcctcccgg gcctgttcga tacagggcaa cagtgctgct gaatccgttt tatcctcatc
     6901 gttgtcaaag ataattcccg atccgcagtc gatattgtcc tgcagccacg caatcagaat
     6961 atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca gtcgaaagtg
     7021 ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac gccataacgc
     7081 cggatgagtg tgacgaccag acgacggaac gtttttgaca gccccaggcg tttaagccgc
     7141 agaaccgggt atgaccacgc atcggtacgt aacagatatc cgctacctgt aaaatgaatc
     7201 cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacaacca ggcgttgtcc
     7261 gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa tggtggcgtg
     7321 cattctgctg aggtctggtc agattcatca acaacacgaa gggagtagcc gttatcgcag
     7381 accatgttaa tcagttcagc gagctcaata ccatcgacgt caacgacaat gcgccccatg
     7441 ttcagtgcct gtacgttaac gctgtcggct tccagtgtca gggccagttt catcatttcg
     7501 cctccggata cttacccaag gtaatgttat ttaccgttct gtaattgtcg cgggtcatca
     7561 ggccggtcgc ccggcgagcc cggaggatat caatactgtt tattaactga gagcggggac
     7621 aggtgctgaa tcccggctgg tcggtacgca ccagcgcgta tttttccacg agaaaattaa
     7681 ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcggcaa
     7741 acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg agcagtgtct
     7801 gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg tcggataatg
     7861 ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg gggttatgag
     7921 gtggttgcga gtgctggtgt cggataaaca gccagataaa cgtaaccaca ggagccgagg
     7981 gtgtcggctt cgcaggtcag cccttttgca ttcagcgtga cggtatgctg atatcgggga
     8041 ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg
     8101 tccagatggt atgcatctgc gtcgctgaag cggcctctga taccggcgcg gtcggcaagg
     8161 taatgcaggc ggttaccctc ctgcaccaga cgggcgccga aacagggcgt cactgtgcag
     8221 ggtagtcccc accagggatg gtcgtcattg tcatcgggat gcgttgtccc ggaaagtgtg
     8281 tctgacacga taaaatcctc acagaaaatc ggtgaatgat agtttaacga tgacgggtca
     8341 gccggcgcgc agaccggctt cggtaaaggt gtattcgttg atgagcagga cctcatccac
     8401 agcggcgtct gagctcagcc agtcgtactc gttttccggg tgacggaaca tcagttcagg
     8461 gtgacgctca tcagccggac atacggacca aaactgtcct tacggcgttc tgcaaacacg
     8521 gccagcacac cgggaatatc ctgcacttca cgaccggtat acgcttcagc actgccgtgc
     8581 cagcggtatt tgccggtaca gaacggaaaa agacgggatg ccggatgctg acggtgaata
     8641 cgcatggctt cgccacgggt gatgattttc atagtgggat acctctgaag acagaagata
     8701 aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt tcggcaaagg
     8761 agaaaacctg gctgccaccg actatcagat ggtccggcac ccggatatcc accaggccca
     8821 gtgcctgtac cagacgttcc gtgataaggc ggtctgcctt gctgggtgtg acttcaccgg
     8881 acgggtgatt gtgtgccagc accacagcag cggcattgtg atacagggcg cgtttaatca
     8941 cttcacgggg atggacttcc gtgcggttga tggtgccggt gaagagggtt tcaccggcaa
     9001 tcagctgatt ctggttgttc agatacagca cccggaactc ttcacgctcc agtcccgcca
     9061 tgttcagaat cagccattct cgtgc
//
LOCUS       DAZFAH010000171         9075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFAH010000171 DAZFAH010000000
VERSION     DAZFAH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.173"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..384
                     /locus_tag="ACHJWB_003815"
     CDS             103..384
                     /locus_tag="ACHJWB_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315375.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(986..2446)
                     /gene="phoQ"
                     /locus_tag="ACHJWB_003816"
     CDS             complement(986..2446)
                     /gene="phoQ"
                     /locus_tag="ACHJWB_003816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4315376.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2446..3117)
                     /gene="phoP"
                     /locus_tag="ACHJWB_003817"
     CDS             complement(2446..3117)
                     /gene="phoP"
                     /locus_tag="ACHJWB_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4315377.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     FFELR"
     gene            complement(3285..4655)
                     /gene="purB"
                     /locus_tag="ACHJWB_003818"
     CDS             complement(3285..4655)
                     /gene="purB"
                     /locus_tag="ACHJWB_003818"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4315378.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4659..5300)
                     /gene="hflD"
                     /locus_tag="ACHJWB_003819"
     CDS             complement(4659..5300)
                     /gene="hflD"
                     /locus_tag="ACHJWB_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4315379.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5336..6442)
                     /gene="mnmA"
                     /locus_tag="ACHJWB_003820"
                     /gene_synonym="trmU"
     CDS             complement(5336..6442)
                     /gene="mnmA"
                     /locus_tag="ACHJWB_003820"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4315380.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6496..6957)
                     /gene="nudJ"
                     /locus_tag="ACHJWB_003821"
                     /pseudo
     CDS             complement(6496..6957)
                     /gene="nudJ"
                     /locus_tag="ACHJWB_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
     gene            complement(6967..7620)
                     /gene="rluE"
                     /locus_tag="ACHJWB_003822"
     CDS             complement(6967..7620)
                     /gene="rluE"
                     /locus_tag="ACHJWB_003822"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4315381.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7792..9042
                     /gene="icd"
                     /locus_tag="ACHJWB_003823"
     CDS             7792..9042
                     /gene="icd"
                     /locus_tag="ACHJWB_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4315382.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSLTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         2250 a         2312 c         2289 g         2224 t
ORIGIN      
        1 attctcataa acagataaaa accgcgaggt cgccgccccg taacgggcca taattccagg
       61 aaggacccga cgacaccaga ctatcagagc gatgggggca caatgacaga agccgaacta
      121 ctgggattaa tccgccgcgt cgccggaatc agccagcagc atgacgaaca ggccacacag
      181 ccggacagcg tgaccgctga aaattatgtg cgtgttgtag cggaagtgat gcgccgtgat
      241 ggtatagaac ttaatggcgt tgatatgcgc aacatacgga accgcgttct tgaaatgctg
      301 gcctacaatc gccgcgttga actgtatcgg gagaaagaaa aaataacgta ccactggaag
      361 aagccggaac ggctgcggcg gtaacttgct gatattcccg ataacgcaaa attgcgctgg
      421 ctacatccct cacatctgag gactgttaat cagaaggccg gaatatattc acaaactacg
      481 aaattttcgt agtttaatta atggtcagat taatcgctaa cccgctgata ttttcggaaa
      541 cctcaatttg aggaagtcgg aagcgtcacc cttcatattc tgaatggtca taatgaccac
      601 gcagaccatc aaggcaacca caacgaacat cgttacggtt ggagctttta ccccactggg
      661 ggaaaggtat tacgataatc ataacaccta ccgaacaggt aaaacccacc agcctgatta
      721 acagttaacc ggaaaaaaaa tccggtagcc ccgttatggg gatccccata tcgacattaa
      781 cgcccctcat gaattggtgc gcttccccct ggaaagatta cccgcctgta tatttcttgt
      841 gtctatttgt tccacgttgt ttcatacagt gcaccgaacg gtgtagttaa tggtgtagtc
      901 aatttgctgt ttttagcact ttttgcagtg tgggttgttt aattaaatca atgggttgcc
      961 ttgctgtggt gataaaagga catatttatt catctttcgg cgcagaatgc tggcgaccaa
     1021 aaatcacctc catccgcgca ccgcccagca tgctctctcc ggcgacgatt ttaccctcat
     1081 attgctcggt gatttcgcgg gctaccgcca gccctacacc ttgcccaggg cgtaaagtat
     1141 caacccgttg accacggtcg aaaatgacct ctcgcttgct taatggaata ccggggccat
     1201 catcctcgac cacaatatag agatgctcgt cggtttgcct tgcagaaatt tcgacaaact
     1261 cgaggcaata tttacaggca ttatccagca cgttgcccat cacctcgaca aaatcgttct
     1321 gctcaccgac aaagctgatc tctggcgaaa tatcgagaga gatattgacc cctttgcgtt
     1381 gatacacttt gttcagcgct gaggtgagat tgtccagcag tggggcgacc ggatgcagct
     1441 cgcggctgag caatgtcccg ccgcgcatac tggcacgatg caggtagtag ccaatttgct
     1501 gtgaaatgcg gctgatttgc tccagcatta ccggctcagc atcactgacg ctcatctttt
     1561 cactacgcag agaacgcagc gtactttgca gcaccgccag tggcgttttc agactatggg
     1621 tcaggtcggt gagcgtcgta cggtatttgt cgtaacgttc gcgttcactt tttaacaatc
     1681 ggttcaggtt tcgtaccaga ctggtcagtt ctcgcgttgt ggctggattg agcaattcgc
     1741 ggttatgttc ttccagttcg cggacttctt ttgccagggc ttcgatgggg cgtaaactcc
     1801 accaggcggc gacccacagc agcgggatca ctaacagcag attggctgag agcacataga
     1861 taaaccagct ccagaccata taggaacttt ttagctccac cggaatggta tccaccacca
     1921 caatggttaa ttttggcatc cgcgatgttg ccgggtagac gtttactgcc accgagtggg
     1981 tcatctccgc gtcgtcatca tcttcccgca cttcctgcaa ctgttgctgt atcgaatgat
     2041 ctccactcag caagaggctg gtatcgttaa catccgcttc aatttcatga aaaccattcg
     2101 atttcagcca gtcaggctgg atcatcttca tcagccaggg cacgtcacgt tgcgcccata
     2161 aaagctgccc gttctcatca taaattagcg tcatggtggg gctttgcttg tcgatatttt
     2221 cgggtaactc gacatgcaac ttattgtttt cccacttcgc aagggtatag aacagattgc
     2281 tctcgccacg taacagccga aacgtagttt tatcgaaact gacgctataa ccgatcagcg
     2341 cgaccattcc gtaggcaagc gaaagcacca gtactaccgc agccgttgcc aacagaaaac
     2401 gtacccgcag tgagagcggg aaaaaaagac gcagtaattt tttcatcagc gcaattcgaa
     2461 aaaatagccc tggccgcgaa cggtagtaat cacttcttgc ggatattgtg cctgaatttt
     2521 tttgcgcaga cgtcccatca gtacatcaat ggtatggctt tcccgcagct ccgcatccgg
     2581 atagagttgg agcattaacg aatctttgct caccactttg ccattattgc gtatcaacgt
     2641 ttccataatg gtgtattcga acgcggtcag tttgatcact tcgtcattaa tagataattc
     2701 acggcgagag agatcaacct gaaacggggg tagcgaaatg acctgtgaag ccaggccgct
     2761 attacgccgc attaatgcct gcattcgcgc catcacctct tcaatatgaa acggtttagt
     2821 cacgtaatca tcagcaccgg cacttaatac ttcgactttg tcctgccagc tttcacgggc
     2881 ggttaatacc agaatcggca gtgaaacatc attgctacgc cagcggcgaa tcagtgacag
     2941 accgtcctcg tcaggcaatc cgagatcgac aatcgcaata tccggtaaat gttcattgag
     3001 ataataatcg gcttctttgg catcttctgc gtcatcgacc tgatgaccag catcctgaat
     3061 ctgaacttta aggtggtgac gtaacaacgc attgtcttca acaaccagta cgcgcatttt
     3121 tatttctccc tgtcttaatt attaaaatag tgtaacgcga ttatgtgttt atgggggtaa
     3181 acattaaata aaccagcggg gaggggaggt aaagtgaaaa aagaaaaagt ggataatttt
     3241 aataaacagg ccggacagca tcgccatccg gcactgatac gagtttattt cagctcatca
     3301 accatggtga tagctcgacc aatatagtta gccggcgtca tcgctttcag gcgggctttc
     3361 tcttcttctg gcaacgccag accatcgata aactgcttca tgccttcggc gtcaacgcgc
     3421 ttaccgcgag tcagctcttt cagcttctcg tacggttttt cgatgccata gcgacgcata
     3481 actgtctgga ttggctcagc cagcacttcc cagttgtgat ccagctcatc cagcagatgg
     3541 tcacggttta cttccagttt gctcacgcct ttcagggtgg attgatacgc aatcagcgca
     3601 taaccgatac ccacgccgag gttacgcagc acggtggagt cggtcaggtc acgctgccag
     3661 cgggaaaccg gcagtttgct tgccagatgc tgcaataccg cgttggaaag gcccagattc
     3721 ccttcggagt tttcgaagtc aatcgggtta actttatgcg gcatggtgga agaaccaatc
     3781 tcaccagcaa tggttttctg tttgaagtgg ttaagggcga tataacccca gacgtcacgg
     3841 tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat caaacaattc ggcaatgtag
     3901 tcgtgcggtt cgatctgggt ggtgtacggg ttccactgaa tacccagcga ggtgacgaac
     3961 tcttcgctga actgatgcca gtcaacttcc gggtaagcgg cgatatgggc gttatagtta
     4021 ccgaccgcgc cgttgatttt gccgaggatc tccacctgat taagctggcg gtactggcgc
     4081 tccatacggt aggcgacgtt tgccatctct ttaccgatgg ttgacggcgt ggctggctga
     4141 ccgtgggtac gagacagcag cgggatatcg cggtactgaa cggcgagatc tttaatgcca
     4201 tcaatcagtt gacgccagta cggcaggatc acttcatcac gcgcggtttt cagcattaat
     4261 gcgtgggaga ggttattgat atcttccgaa gtacaggcaa agtggatgaa ttcagaaacc
     4321 gcgtgcagtt ccgggatctc cgccactttt tctttcagga aatactcaac cgctttaacg
     4381 tcgtggttag tggtacgctc gatggttttg atgcgtgcgg catcttcttc gctgaaattg
     4441 gcgacaattg catcaaggta accgattgcg tcggcagcaa aagcaggaac ttccttgatc
     4501 gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa cttgtacacg gaatttcagc
     4561 aaaccatatt cgctgaaaat cccgcgcagc gcgctgactt tatcgccgta gcgtccatcg
     4621 acaggggaaa cggcggtcag tgaggataat tccatagatc acaactccgg ggttaaatga
     4681 gcaagaattt gttttgcctg agtggtcagg cgattacgag aaaacatcag ttgcagacgt
     4741 ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag ggttgcgcga
     4801 actttcgcct gcacttgtgg gctttgcagt acagcagggg aaccggtgac ctgaatgcgc
     4861 gggccaagcg ggctgatcac atcaacatag atagcagcca tcgcgctcat cagcgtttcg
     4921 gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt gcccagagtg
     4981 tcgagcgcgc ctttcgctga ggagagtttg cgctcaagca ccatcaagct gagtgtgtag
     5041 cgggttaatt cggcgtttaa gccctggcga ctgctggcat tgagcacgcc gagcagggtt
     5101 tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt cgagctgggg
     5161 ttcatatcaa taatactgtt gagtgagacg tgtagcgcat cggcatcaca atgcccctgg
     5221 tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac cggccagggc gagggtgatg
     5281 tcatagtaat tctttgccac gttcactgct tccttgtttt aagtaaagat aataatcaga
     5341 ccggcagcgg cagacgctgc tcaataatac cgccaccgag gcacacttca ccgttataga
     5401 agacggcaga ctggcccggc gtcacggcgg caaccggttc atcgaaaatc acttcaatgc
     5461 gatcatcgtc cagcgccttg acggtgcaag ggatatcggt ctggcgatag cgggttttta
     5521 ccgtgcaacg catagtgccg gtgaatggtt cgcgatcgac ccagtgcaac tgctgggcaa
     5581 tcaacccgac agacatcagc cgcgggtgtt catggccctg agcgacaacc agaatgttgt
     5641 tttcgacgtc tttgtccacc acataccacg gttcttcggt accttctttg gtgccaccga
     5701 tacccagacc tttacgctga ccgagagtgt gatacatcag cccctggtgc tcaccaattt
     5761 catcgccatc gacggtaatg attttgcccg gttgcgccgg gagataacgg cccaggaact
     5821 cgcggaattt acgttcgcca atgaagcaaa tgccggtaga gtcttttttc ttcgcggtga
     5881 ccagaccaag atcttcagca atcttacgca cctgcggttt ttccagttcg ccgaccggga
     5941 acaggctttg cgcaatctgc tcatggctga gcgtataaag gaagtagctc tggtctttat
     6001 tgctgtccag accacgcagc aggcggctct tgccatcgac atcggcccga cgtacgtaat
     6061 gaccggtagc gatataatcg gcacctaaat cttcggcggc aaattcgagg aaggctttaa
     6121 atttgatctc tttgttgcac agaatatccg gattcggcgt gcgaccggct ttatattcgg
     6181 caaggaacag ttcgaagacg ttgtcccagt actcggcagc aaagttaacg gtgtgcagtt
     6241 caatgccgag cttgtcgcag acagcctggg catcagccag atccgctgcc gctgtgcaat
     6301 attcctcacc gtcgtcttct tcccagttct tcataaacag gccttcgacc tgatatccct
     6361 gttgttgcaa cagccaggca gaaacggagg aatcgacacc gccggacatg ccgacgatta
     6421 ctttttttgc ggtttcagac attggatcac tcacgacatt gaacttcaag gcggcgtatt
     6481 ctatcacgca gccttttaga tgacaccctt tgtaaaaggc cagttaaaat cgccaatcat
     6541 ctccagcgga taacgttgcc cgctttgata acaacgaata ctttctgcca ccagcggcga
     6601 acgaagattt gacgcctgta aaatttcttc ggcgctgacc caacggcagc agtcgatatc
     6661 gctgtcatga ggctgggtcg ggcatatttg ctcaagctca atggcaaaga ggaaacgcaa
     6721 aaacggcgtt ttatctggcg caatccactg atgcatacga ataaagtgtt gcggttgcgc
     6781 gctgatgccg gtttcttccc atagctcacg ggcggcagct tccacttagg tttcatcggc
     6841 ttccagatgc ccggcaggtt ggttccataa cgctttacca ttaatcgtct cttcaacgac
     6901 taaaaattta ccctctgcgt gcactacgca agcaacggta acgtgcggtt taaacattgt
     6961 gcatccttaa tctgtcactt ctcgccattc gccattggca agattatcca aagagtaatc
     7021 tcccatcgca tagcgaatca gtcgcagcgt ggggaaacca acatgggcgg tcatgcggcg
     7081 cacctggcga ttacgtcctt catataaggt gatcttcagc cagctggtgg gaatactttt
     7141 gcgttcacga attggtggat tccgcggcca taaccacgct ggttcgtcaa ccagctccgc
     7201 gccggcgggc agggtagggc catcatttaa ggttacgcca ttgcgcaagg cttcaagtgc
     7261 gtcttgtgtg ggaatacctt ccacctgcac ataatagatt ttcccggtgc gtttgcccgg
     7321 ctgggttaaa cgcgcctgca acgcgccgtt attggtcagc accagtaacc cttcgctatc
     7381 gcggtcaaga cgacctgctg cataaacgcc ctgaaccggg atgaattctt ttaatgtttt
     7441 gcgtccggct tcatcggtga actgcggaag aacatcgtag ggtttattga acaggatcac
     7501 acgcgtgggc tggttttcag gtttacgcct ggtagaacgt tgcgagctga atcgcttaac
     7561 ctggtgattt ctaaaagaag ttttttgcat ggtattttca gagattatga attgccgcat
     7621 tatagcctaa taatgcgcat ctttcatgac ggcaaacaat agggtagtat tgacaagcca
     7681 attacaaatc attaacaaaa aattgctcta aagcatccgt atcgcaggac gcaaacgcat
     7741 atgcaacgtg gtggcagacg agcaaaccag tagcgctcga aggagaggtg aatggaaagt
     7801 aaagtagttg ttccggcaca aggcaagaag atcaccctgc aaaacggcaa actcaacgtt
     7861 cctgaaaatc cgattatccc ttacattgaa ggtgatggaa tcggtgtaga tgtaacccca
     7921 gccatgctga aagtggtcga cgctgcagtc gagaaagcct ataaaggcga gcgtaaaatc
     7981 tcctggatgg aaatttacac cggtgaaaaa tccacacagg tttatggtca ggatgtctgg
     8041 ctgcctgctg aaacccttga tctgattcgt gaatatcgcg ttgccattaa aggtccgctg
     8101 accactcctg ttggtggcgg tattcgctct ctgaacgttg ccctgcgcca ggaactggat
     8161 ctctacatct gcctgcgtcc ggtacgttac tatcaaggca ctccaagccc ggttaaacac
     8221 cctgaactga ccgatatggt tatcttccgt gaaaactcgg aagacattta tgcgggtatc
     8281 gaatggaaag cagactctgc cgacgccgag aaagtgatta aattcctgcg tgaagagatg
     8341 ggcgtgaaga aaattcgctt cccggaacat tgcggtatcg gtatcaagcc gtgttctgaa
     8401 gaaggcacca aacgtctggt ccgtgccgcg attgaatacg caattgccaa cgaccgtgac
     8461 tctttgaccc tggtgcacaa aggtaacatc atgaagttca ccgaaggcgc gtttaaagac
     8521 tggggctacc agctggcgcg tgaagagttt ggcggtgaac tgatcgacgg tggcccgtgg
     8581 ctgaaagtta aaaacccgaa cactggcaaa gagatcgtca ttaaagacgt gatcgctgat
     8641 gcattcctgc aacagatcct gctgcgtccg gctgaatatg atgttatcgc ctgtatgaac
     8701 ctgaacggtg actacatttc tgacgccctg gcagcgcagg ttggcggtat cggtatcgcc
     8761 cctggtgcaa acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg tactgcgccg
     8821 aaatacgccg gtcaggacaa agtaaaccct ggctctatta ttctctccgc tgagatgatg
     8881 ttacgccata tgggctggac tgaagccgca gacctgattg ttaaaggtat ggaaggcgca
     8941 atcaatgcga agaccgtaac ctatgacttc gagcgtctga tggaaggcgc taaactgctg
     9001 aaatgttcag aatttggtga tgcgatcatc aagaacatgt aatcactaca tgtgtttaat
     9061 attgcaacgg gcgta
//
LOCUS       DAZFAH010000172         8954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFAH010000172 DAZFAH010000000
VERSION     DAZFAH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.174"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1048
                     /gene="yiaK"
                     /locus_tag="ACHJWB_003824"
     CDS             50..1048
                     /gene="yiaK"
                     /locus_tag="ACHJWB_003824"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO4315383.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1060..1527
                     /gene="yiaL"
                     /locus_tag="ACHJWB_003825"
     CDS             1060..1527
                     /gene="yiaL"
                     /locus_tag="ACHJWB_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO4315384.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            1645..2118
                     /gene="yiaM"
                     /locus_tag="ACHJWB_003826"
     CDS             1645..2118
                     /gene="yiaM"
                     /locus_tag="ACHJWB_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO4315385.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            2121..3398
                     /gene="yiaN"
                     /locus_tag="ACHJWB_003827"
     CDS             2121..3398
                     /gene="yiaN"
                     /locus_tag="ACHJWB_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO4315386.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            3411..4397
                     /gene="yiaO"
                     /locus_tag="ACHJWB_003828"
     CDS             3411..4397
                     /gene="yiaO"
                     /locus_tag="ACHJWB_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO4315387.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            4401..5897
                     /gene="lyxK"
                     /locus_tag="ACHJWB_003829"
     CDS             4401..5897
                     /gene="lyxK"
                     /locus_tag="ACHJWB_003829"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO4315388.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            5894..6556
                     /gene="ulaD"
                     /locus_tag="ACHJWB_003830"
     CDS             5894..6556
                     /gene="ulaD"
                     /locus_tag="ACHJWB_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4315389.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALASSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            6549..7409
                     /gene="sgbU"
                     /locus_tag="ACHJWB_003831"
     CDS             6549..7409
                     /gene="sgbU"
                     /locus_tag="ACHJWB_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO4315390.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            7403..8098
                     /gene="araD"
                     /locus_tag="ACHJWB_003832"
     CDS             7403..8098
                     /gene="araD"
                     /locus_tag="ACHJWB_003832"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4315391.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(8130..8945)
                     /locus_tag="ACHJWB_003833"
     CDS             complement(8130..8945)
                     /locus_tag="ACHJWB_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4315392.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFILLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         2076 a         2355 c         2393 g         2130 t
ORIGIN      
        1 attgaaattt taagccaaaa aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac
       61 atttgagcag ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg ttgacagcga
      121 aacggctgac gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca
      181 cggcgttaat cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga
      241 tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc
      301 gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca
      361 cggtattggt ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg
      421 ctggcaggcg gcggaaaaag gctatattgg catttgctgg accaactcca tcgccgtaat
      481 gccgccgtgg ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg tcgccattcc
      541 ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga
      601 agttaaccgc ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg atgagggcaa
      661 tttgaccaaa gaacctggcg ttatcgagaa gaatcgccgc attttgccga tgggctactg
      721 gaaaggttct ggcatgtcga ttgtgctgga tatgatcgct actctccttt ccgacggcgc
      781 atcggttgcc gaagtcaccc aggacaacag cgacgaatac ggcatttcac aaatttttat
      841 tgccattgaa gtggacaagc ttatcgacgg tcccacccgc gatgccaagc tgcaacgtat
      901 catggattac gttactaccg ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg
      961 ccacgaattt actaccctgc tggccgaaaa ccgccgtaac ggtattaccg ttgatgacag
     1021 cgtgtgggcc aaaatccagg cgttataagg agataagtca tgatttttgg acatatcgcg
     1081 cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct
     1141 accgatttca acgccctgga gccgggcgtt gtcgaaatcg acggcaaaaa tatttatgcg
     1201 caaattatcg atttaaccac acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg
     1261 tatatcgata ttcagtttct ggcgtggggt gaagagaaaa tcggcattgc tattgatacg
     1321 ggaaataata aagtcagcga atcactatta gagcagcgcg atattatttt ttatcatgac
     1381 agtgaacatg aatcatttat agaaatgata ccgggcagct acgcaatttt ctttccgcag
     1441 gatgttcacc gaccaggttg tattttgcaa acagcctctg agatacgaaa aattgtggtt
     1501 aaagtcgcat taacggcgct gaattaatcg aataatcgtc tacgccagaa cggctgggta
     1561 atgttattgc tctaactata ttaataattt aggttgctgc acatgcaact tgaattatga
     1621 cagacatagc ctcaaggaat agctatgaaa aaaatacttg aagcaatact ggcgattaat
     1681 ctcgccgtac tttcctgtat tgtgtttata aacattattt taagatatgg ttttcagaca
     1741 agcattttat ctgtagatga attgtcacgt tatttatttg tctggttaac atttattggc
     1801 gcgattgtgg cttttatgga taacgcccac gttcaggtca cctttctggt ggagaaactc
     1861 tcccccgcat ggcaacgacg agtcgctctg gttacccatt ccttaatctt atttatttgt
     1921 ggcgcactgg cctggggcgc aacgctaaaa acaattcagg actggagcga ttattcaccg
     1981 atcctcggtt tacccatcgg cctgatgtat gccgcttgtc tgcccaccag cctcgttatc
     2041 gcattctttg aactgcgtca tttatatcaa ctcatcacgc gtagcaattc tctaacgtcg
     2101 ccaccgcaag gagcttaatc atggctgtgc tgatttttct gggctgtctg ttgggtggta
     2161 ttgctatcgg tttgcctatt gcctgggcac tgttgttgtg cggggcggcg ttaatgttct
     2221 ggctggacat gtttgatgtc cagatcatgg cacaaacgct ggtgaacgga gccgatagct
     2281 tctccctgct ggcgattccg ttctttgtgc tggcgggtga aatcatgaat gcgggcggct
     2341 tgtcaaaacg cattgttgac ctgccgatga aactggtggg gcataaacca ggcgggctgg
     2401 gctacgtcgg cgtgctggca gctatgatta tggcgagcct ttctggttct gccgttgcgg
     2461 ataccgccgc cgttgccgct ctactggtgc cgatgatgcg cagcgccaac tacccggtca
     2521 accgggcggc ggggctgatt gcctctggcg gcattatcgc gccaattatt cccccttcca
     2581 ttccgtttat tatcttcggc gtttccagcg gattatccat cagtaagctg tttatggcgg
     2641 gcattgcccc tggcatgatg atgggcgcaa cgctgatgct tacctggtgg tggcaggcca
     2701 gtcgccttaa tttaccccgc cagcaaaaag caacgatgca ggaaatctgg cactcttttg
     2761 tctccggtat ctgggcgctg tttcttccag tcatcattat tggcgggttt cgctccggtc
     2821 tgtttacccc taccgaagcc ggggccgttg ctgctttcta tgcgctgttt gtcgccacag
     2881 ttatttaccg tgaaatgacc ttcgccacac tttggcatgt actcattggc gcggcgaaaa
     2941 ccacctcagt ggttatgttt ctggtggcct cggcacaagt ttccgcctgg ctgattacca
     3001 ttgctgaact gccgatgatg gtttctgatt tactgcaacc gttggtcgat tcaccgcgtc
     3061 tgctgtttat cgtcattatg gtggctatcc tgattgtcgg catggtcatg gatttaacgc
     3121 cgacggtatt aattcttacc ccggtattaa tgcctttagt taaagaggca ggaatcgatc
     3181 cgatttattt cggtgtcatg tttatcatta actgctcaat cggcttaatt acaccgccta
     3241 tcggtaatgt gcttaacgtt atttcagggg tggcaaaact caaattcgat gatgcagtca
     3301 gaggcgtttt cccttacgtc ctcgttttat attccttatt agtcgtattt gtttttattc
     3361 ccgatctcat catcctccct ttgaaatgga ttaattaaaa ggaaaatatt atgaaattac
     3421 gctctgtaac ctacgcatta ttcattgctg gcctggctgc attcagcaca tcttctctgg
     3481 cggcacaatc attacgtttc ggttatgaga catcacaaac cgactcgcaa catattgcgg
     3541 cgaaaaaatt caatgaatta ttacaggaaa aaaccaaagg cgaactgaaa ttaaaattgt
     3601 ttccggatag caccctcggt aacgcgcagg cgatgatcag cggcgtacgt ggcggcacca
     3661 tcgatatgga aatgtccggc tcgaataact ttgccgggtt atcaccagtg atgaacttgc
     3721 ttgatgtccc tttcctgttc cgcgataccg ctcacgcgca taaaacgctc gacggcaaag
     3781 tcggtgatga tctgaaagcc tcacttgaag gtaaaggact aaaagtactg gcctactggg
     3841 aaaacggctg gcgcgatgtc accaactcgc gcgcaccggt taaaaccccc gccgacctga
     3901 aagggctgaa aattcgcacc aacaatagcc cgatgaatat cgccgcattc aaagtctttg
     3961 gcgctaaccc gatcccgatg ccgtttgccg aagtctatac cgggctggaa acccgcacta
     4021 tcgacgctca ggaacacccg atcaacgtcg tctggtcagc aaaatttttc gaagtgcaga
     4081 agtacctttc tctgacgcac cacgcctatt ccccgcttct ggtggtgatc aacaaagcga
     4141 agtttgatgg cttaagcccg gagttccagc aagcgctaat ttcatctgcg caggaagcgg
     4201 gtaactacca gcgcaaactg gttgccgaag atcagcaaaa aatcatcgac ggcatgaaag
     4261 aagcgggcgt ggaagtcatc accgatctcg accgcaaagc ctttagcgac gctctgggta
     4321 atcaggttcg cgacatgttt gtaaaagatg ttccgcaggg tgctgatcta ctgaaagccg
     4381 tggatgaggt gcaataaacc atgacgcaat actggctggg gttagattgt ggcggtagct
     4441 ggctgaaagc cgggctgtat gaccgcgaag gccgggaggc aggcgtgcag cgcctgccgc
     4501 tgtgcgcatt aagcccgcag ccaggttggg cagagcgcga tatggcagaa ctgtggcaat
     4561 gctgcatggc tgtcattcgc gaactgctta cccattccgg cgttagcggg gaacaaattg
     4621 tcggtatcgg tatctccgca caaggaaaag gcttgttttt gctggataaa aacaacaagc
     4681 cgctcggtaa cgccatttta tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc
     4741 aggaagatgg catcccggaa aaactctacc cgctgacccg acaaaccttg tggaccgggc
     4801 atccggtatc gctgttacgt tggcttaaag agcacgaacc ggaacgctac gcgcaaattg
     4861 gctgcgtgat gatgacacac gactacctgc gctggtgttt aaccggcgtc aaaggctgtg
     4921 aagagagcaa tatttccgag tccaatctct acaacatgag tcttggggaa tatgacccgt
     4981 gcctcaccga ctggctgggg attgctgaaa tcaatcacgc cctgccgcct gttgtcggat
     5041 ctgccgaaat ctgcggggag atcaccgctc agacagccgt actgaccggt ctgaaagcgg
     5101 gtacgcccgt tgtcggcggc ctgtttgatg tggtttccac cgcactctgc gccgggatcg
     5161 aagacgaatt tacccttaat gcggtgatgg gcacctgggc ggtgaccagc ggcataaccc
     5221 gcggtttacg tgacggtgaa gcgcatccgt atgtctatgg tcgctacgtt aacgatggtg
     5281 aatttatcgt tcacgaagcc agccccacct cttccggcaa cctcgaatgg tttaccgcac
     5341 agtggggaga aatctcgttt gatgagatca accaggccgt tgccagcttg ccgaaggccg
     5401 gtggcgatct ctttttcctg ccgttcctgt acggcagcaa cgccggactc gagatgacca
     5461 gcggtttcta cgggatgcag gccattcaca cccgcgcaca cctgttgcag gccatttatg
     5521 aaggggtggt gttcagccat atgacccacc tcaaccggat gcgcgaacgt tttactgatg
     5581 tgcacaccct gcgcgtcact ggcggcccgg cgcactccga tgtctggatg caaatgctgg
     5641 cggacgtcag cggtctgcgt atcgagctgc cgcaggtgga agaaaccggc tgttttggcg
     5701 cggcacttgc cgcccgtgtc ggcaccggag tttatcgcga tttcagcgaa gctcaacgtg
     5761 acttacagca cccggtacgc accctgctgc cggatatgac cgcccatcag ctttaccaac
     5821 aaaaatacca acgctatcag catctcattg ccgcacttca gggctttcac gcccgcatca
     5881 aggagcacat attatgagcc gaccacttct gcaactggca ctcgaccact catcacttga
     5941 agccgcacag cgcgacgtga cgcagttaaa agacagcgtc gatattgtcg aagcgggcac
     6001 cattctctgt ttaaacgaag ggcttggcgc ggtgaaagcc ttgcgcgaac agtgccctga
     6061 caaaatcatc gttgccgact ggaaggtcgc cgacgctggt gaaacgctcg cgcaacaggc
     6121 atttggcgca ggcgctaact ggatgaccat catctgcgcc gcaccgctcg cgacggtgga
     6181 aaaaggccac gcgatggcgc aacgctgcgg tggtgaaatt cagatcgagc tgttcggtaa
     6241 ctggacgctg gacgacgccc gcgactggca tcgtattggc gtgcggcagg cgatttatca
     6301 tcgcggtcgt gatgcacagg ccagcgggca acagtggggc gaagccgatc tggcacgcat
     6361 gaaggcgctt tccgatatcg gccttgagct ttccattacc ggcgggatta cccctgctga
     6421 cctgccgctg tttaaagata ttcgcgtgaa agcgtttatt gccgggcgag cactggcaag
     6481 ctcagcaaat ccggcgcagg tggctggcga tttccatgcg caaatcgacg ctatctgggg
     6541 aggcaagcgt gcgtaatcat ccgttaggga tttatgaaaa ggcgctggcg aaagatctct
     6601 cctggccgga gcggctggtg ctggcaaaaa gctgcggttt tgattttgtc gaaatgtcgg
     6661 tggatgaaac cgacgaacgg ctttcacgtc ttgactggag caccgcacaa aggacttcgc
     6721 tggttgccgc gatgatcgaa acgggtgttg ggatcccgtc gatgtgcctg tccgcacatc
     6781 gtcgctttcc ttttggtagc cgtgacgacg cggtacgcca gcgagcacgg gagatcatga
     6841 gcaaagcgat tcgtctggca cgcgatctcg gcatacgcac catccagctg gcgggctacg
     6901 acgtctatta cgaagaccac gatgaaggca cccggcaacg ttttgctgaa gggctggcgt
     6961 gggcggtcga acaggcggcg gcatcacaag tcatgctagc ggtggagatt atggacaccg
     7021 cgtttatgaa ctccatcagt aaatggaaaa agtgggacga gatgctcgcc tcgccgtggt
     7081 tcaccgtcta cccggacgtc ggcaacctca gcgcctgggg caatgatgtt cccgccgaac
     7141 tgaaactggg cattgaccgt atcgccgcga tccacctgaa agacacccag ccagttaccg
     7201 agcaaagtcc cggacagttc cgcgacgtgc cgtttggcga aggctgcgtc gatttcgttg
     7261 gcatcttcaa aacgctgcat aaactgaact atcgcggttc tttcctgatt gagatgtgga
     7321 ccgaaaaagc caaagagccg gtgctggaga ttattcaggc gcggcgttgg attgaagcgc
     7381 gtatgcagga ggctggattt atatgttaga gcaactgaaa gccgacgtgc tggcggcgaa
     7441 tctggcgctt cccgctcacc atctggtgac gttcacctgg ggcaatgtca gcgcggtaga
     7501 cgaaacgcgg caatggatgg taatcaaacc ttccggcgtc gagtatgacg tgatgaccgc
     7561 cgacgatatg gtggtggttg agatagccag cggcaaggtg gtggaaggta gcaaaaagcc
     7621 ctcgtccgac acgccaacgc atctggcgct ttaccgtcgc tacgccgaaa tcggtggcat
     7681 tgtgcatacc cactcacgcc acgccaccat ctggtcgcag gccgggctgg atctccccgc
     7741 ctggggcacc acgcacgctg attatttcta tggtgccatc ccctgcacgc gacagatgac
     7801 cgcagaggag attaacggcg aatatgaata tcagaccggc gaagtgatca ttgaaacctt
     7861 cgaagaacgt ggcaggagtc cggcacaaat cccggcggtg ctggtgcatt ctcacggccc
     7921 gttcgcatgg ggtaaaaacg ccgccgatgc cgtgcataac gccgtagtac tcgaagaatg
     7981 cgcctatatg ggtctgttct cgcgccagct tgcgccgcag ctccctgcga tgcaaaacga
     8041 actgctggat aagcactacc tgcgtaagca tggagacaat gcctattacg ggcagtaatc
     8101 cctcacaccg gggcttcatc gccccggcac tacgaattga tatgttcctt gctgtaacgt
     8161 cgcttccacg ccgctggcgt taaaccggtg tgcttgcgaa aaatctgccg gaaatagcca
     8221 acgtcattaa acccgcacaa cgcagccact tcagtgagcg atcgcgcttc gctaattaac
     8281 agcttttcgg cttcgcgcac tcgctggcga tggattgctt ctgttaacgt cagatgaaac
     8341 acccgccggt agacgcgccc aagataatcg gcattgcagt acagctcgcg ggccagaagc
     8401 gccgtagaaa gcgggcgatg atattgcgtg cggatcagtt tctgcgcttt ccaggcaagg
     8461 gtggtgcctg cgccatcggc ctgttcatca agacgcgcgt cacgggtgag ctgctgcaaa
     8521 atcagcagca aaataaactc cagcgccgga gtgcgttgat gcttttcctg ttcgcttaag
     8581 aactggcgaa acagggccac gagcgcctgc ggatcggcaa ccttcgtttg ttgcggcacg
     8641 ctaagatgag tgagccagcg cgggtcgcta tccggcgctt taacctcaaa atgcagccag
     8701 taaaatttga gatcggcggg aaaatcctcc accccaacat gttgtcgatt cggccagagt
     8761 aataaactct ctcccgcatc gaccagaaat atccgttctt cttcctgaat cgttaatcga
     8821 cctttttcga caaagataat ttcccatgac tccagccgcc ttgcaggatg cctccctacg
     8881 ccgcgggaaa taaataatcc accattttgc actttaatcg gtaacgtcat ggataattcg
     8941 agcattgata tgta
//
LOCUS       DAZFAH010000173         8845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFAH010000173 DAZFAH010000000
VERSION     DAZFAH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.175"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1095
                     /gene="emrK"
                     /locus_tag="ACHJWB_003834"
     CDS             <1..1095
                     /gene="emrK"
                     /locus_tag="ACHJWB_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4315393.1"
                     /translation="LFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTV
                     VNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASA
                     RIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMN
                     TPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVP
                     ARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNTFSLLPAQ
                     NATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTS
                     MPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1095..2633
                     /gene="emrY"
                     /locus_tag="ACHJWB_003835"
     CDS             1095..2633
                     /gene="emrY"
                     /locus_tag="ACHJWB_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4315394.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2741..4069)
                     /gene="dsdA"
                     /locus_tag="ACHJWB_003836"
     CDS             complement(2741..4069)
                     /gene="dsdA"
                     /locus_tag="ACHJWB_003836"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4315395.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4087..5424)
                     /gene="dsdX"
                     /locus_tag="ACHJWB_003837"
     CDS             complement(4087..5424)
                     /gene="dsdX"
                     /locus_tag="ACHJWB_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFO4315396.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            5642..6577
                     /gene="dsdC"
                     /locus_tag="ACHJWB_003838"
     CDS             5642..6577
                     /gene="dsdC"
                     /locus_tag="ACHJWB_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFO4315397.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            complement(6673..6747)
                     /locus_tag="ACHJWB_003839"
     tRNA            complement(6673..6747)
                     /locus_tag="ACHJWB_003839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6712..6714),aa:Arg,seq:cct)
     gene            complement(6823..7755)
                     /locus_tag="ACHJWB_003840"
     CDS             complement(6823..7755)
                     /locus_tag="ACHJWB_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4315398.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVEREGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8049..8804
                     /gene="mlaA"
                     /locus_tag="ACHJWB_003841"
     CDS             8049..8804
                     /gene="mlaA"
                     /locus_tag="ACHJWB_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4315399.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         2347 a         2057 c         2087 g         2354 t
ORIGIN      
        1 ttatttattg cgttttcagg tgcctatgcc tattggtcaa tggaattaga agacatgatt
       61 agtacagatg acgcctatgt cacggggaat gcagatccaa tttctgcaca agtctcaggt
      121 agtgtcactg tcgttaatca taaagatacg aactacgttc gacaaggtga cattttagtt
      181 tcactggata aaactgatgc cactatcgca ctcaataaag ctaaaaataa tctggcaaat
      241 attgttcggc aaacgaataa actatactta caggataaac aatacagtgc cgaagtcgct
      301 tcagcacgta ttcagtatca acaatcttta gaagattata accgtcgagt gccgttagcg
      361 aagcaggggg ttatttcaaa agaaacgctg gagcatacca aagatacgtt aataagtagc
      421 aaagcggcat tgaatgccgc tatccaggct tataaagcga ataaagcttt agtaatgaac
      481 acaccattaa accgtcagcc acaagtcgtt gaagcggcgg atgcaactaa agaagcctgg
      541 ttggcgctta aacgtacgga tattaagagt ccggttaccg gctatattgc ccagagaagt
      601 gttcaggtcg gcgaaacagt gagccccgga caatcgttaa tggctgtcgt accggcacgt
      661 caaatgtggg ttaatgccaa ctttaaagaa acacaactca cggatgtgcg gattggtcaa
      721 tcggtcaata ttatcagcga tctttatggt gaaaatgttg tgtttcatgg tcgggtgaca
      781 gggatcaata tgggaaccgg caatacgttc tccttattac ctgcacaaaa tgcgacaggg
      841 aactggatca aaatcgttca gcgtgtaccg gttgaagttt ctcttgatcc aaaagaactc
      901 atggaacatc ccttgcgtat tggtttatcg atgacagcaa ctattgatac gaagaacgaa
      961 gacattgccg agatgcctga gctggcttca accgtgacct ccatgccggc ttataccagt
     1021 aaggctttag ttatcgatac cagtccgata gaaaaagaaa ttagcaacat tatttcgcat
     1081 aatggacaac tttaatggca atcactaaat caactccggc accattaacc ggtgggacgt
     1141 tatggtgcgt cactattgca ttgtcattag cgacatttat gcaaatgttg gattccacta
     1201 tttctaacgt cgcaataccg acaatatctg gctttctggg agcatcaaca gacgaaggca
     1261 cctgggttat cacctcgttt ggtgtagcaa atgccattgc gatccctgtt actggcaggt
     1321 tggcacaaag aataggcgaa ttaagattat ttttactttc agtaaccttt ttttcgctgt
     1381 cttcattaat gtgtagccta tcgaccaatc ttgatgtgct gatatttttt agagtcgttc
     1441 aggggttaat ggcggggccg ttaattccac tgtcacagag tttattatta aggaattatc
     1501 cgccagaaaa aagaacattt gctctggcat tatggtcaat gaccgtgatt atcgctccga
     1561 tatgtgggcc gatattgggc ggttatattt gtgataactt tagctggggt tggatatttt
     1621 taatcaatgt ccctatgggg attatcgtcc tgacattatg cttaacctta cttaaaggaa
     1681 gagaaactga gacttcaccg gtcaaaatga atctaccagg actgaccctg ttagtgctcg
     1741 gtgttggtgg cttgcaaatt atgcttgata aagggcgcga tctggattgg ttcaactcga
     1801 gtacaataat aatattaaca gtagtatcag ttatttctct gatctcttta gtcatttggg
     1861 agtcgacctc agagaatccg attcttgatc tcagtttgtt taagtctcgt aatttcacca
     1921 ttggtattgt gagtatcacc tgcgcgtatt tattttactc tggagcgatc gtccttatgc
     1981 cgcagttact ccaggaaacg atggggtata atgcgatatg ggccgggctt gcttatgcgc
     2041 ccatcggcat catgccacta ttgatttcac ctttgatagg acgttatggc aataaaatag
     2101 acatgcgggt gttagtgaca ttcagttttt tgatgtatgc ggtttgctat tactggcgtt
     2161 ctgtgacatt tatgccaacg attgatttta caggcatcat tttgccacag tttttccagg
     2221 gattcgccgt tgcctgtttc tttttaccct taacaacgat ttcgttttca ggcttgccag
     2281 ataataaatt tgccaatgcc tcgagtatga gtaatttttt tcgtaccttg tcaggatcag
     2341 ttggtacgtc gttgacaatg acgctgtggg gacgacgcga atcgttacac catagtcagt
     2401 tgacagcaac catcgatcaa tttaaccccg tgtttaattc atcgtcacaa attatggata
     2461 aatactatgg ttcgctttca ggagttctta atgaaattaa taatgaaata acccagcagt
     2521 cactttctat ttctgcaaat gagattttcc gtatggcggc tattgctttt atcttactta
     2581 cggttttggt ttggtttgcg aaaccgccgt ttacagcgaa aggcgttggg tgatgataaa
     2641 aggagggggt tatagcgggt aagaccgggg atcttaagtc tgcgccatcg gcagcatcgc
     2701 tcacccaggg aaaggattgc gatgctgcgt tgaaacgtta ttaacggcct tttgccagat
     2761 attgattcat ctcttcttcc ggcaccattc cacctcccgt cgcccacacc agatgagtgg
     2821 cattacgcaa ttgttctgcg ctgaaaccgt gcatctgttg gtaacttact gatgcacaca
     2881 cgcgctgagg tccggccata cccgccagtg ctgaaggttc aagacgaata ccttcttcct
     2941 gcgccagcca gccaagcatg tcatacatgg tttgatcgct aagggtatag aagccatcca
     3001 gcagacgctc cattgcccgc ccgacaaagc ctgatgcgcg accaactgca aggccatccg
     3061 ctgcggtaag gttgtcgata ccaatatcct gaacagaaat ctgatcgtgt aatcctgtat
     3121 ggacgcctaa caacatacaa ggggagtgcg ttggttcggc aaaaaagcag tgaacatgat
     3181 cgccaaacgc cagtttaagc ccgaatgcga cgccaccagg accaccgcca acaccacacg
     3241 gcagatagac aaacagaggg ttatcagcat cgacgatacg gccttgctgg gcaaattgcg
     3301 ctttaagacg ctggccagcg acggaatacc caaggaacaa cgtgcgggaa ttttcgtcat
     3361 caataaagaa acagttcggg tcagactgcg ctgctttacg tccttcctcg acggcaacac
     3421 cataatcttg ctcatattcc acgaccgtaa cgccatggct gcgcagtttc gcttttttcc
     3481 atgcccgggc atcagcagac atatgaactg tcaccttaaa gccaatgcgg gcgctcataa
     3541 tgccgattga taaccccaga tttccggttg agcccacagc aatgctgtat tggctaaaga
     3601 actgtttaaa ctccggagaa agcagtttgc tgtagtcatc atcaagcgtc agcaaccccg
     3661 cttccagagc cagtttttct gcgtgtgcca ggacttcata aatcccgccg cgtgctttta
     3721 tggagccgga aatgggcaaa tggctatctt ttttcagtaa cagttgcccg ctgatcggtt
     3781 gctgatattc tttttccagc cgtttttgca tagctggaat ggcaaccagt tctgattcaa
     3841 taatcccccc agtggcagca gtttcaggaa atgcttttgc cagatagggt gcaaaacggg
     3901 ataagcgcgc atgggcgtcc tgaacatcct gttcggtcag gccaacataa ggtaaacctt
     3961 cagccaatga ggtcgtgcca ggattaaacc aggtggtttc tttaagagca accagatcct
     4021 ttaccaacgg atactgggcg atgagcgagt tcattttagc gttttccata gtacgtctct
     4081 ttgcgcttag atgataaatg acagcaggaa tgtgcccgcc agagcgacga ctgaagcgat
     4141 aaatgtcgct gtcgtatagt atttaaatgt ttcattgagc gtagcgccgc aatattgctt
     4201 cactagccag aaaagcgagt ccgtaacgat cgtgcagcca attgcacctg aaccgatagc
     4261 aatcgcaata atttccgggc tgatgtcggg atacagcggc agcatgggtg caacaattgc
     4321 cgttgcaccc atcattgcca cggtagcgga gcccactgcc gcatgcagaa taagagccac
     4381 taaccaggcc agaagaatcg ggtgcatatg catattggag agaataactg ccagcgtatc
     4441 agcgagactg ctgcttttta aaatggcgtt gaatgcgcct ccggccccga taatcagcaa
     4501 aatattagca atagaaccga agccattttc cgtatgtgtg agcatcgtcc ccatgctcat
     4561 atgctggcgt atacccaaca cataataggc gacaaacacg gcgataaaca tggcagtgat
     4621 agggttgcca ataaactcaa ccaagatata caaaccactc tcacgcgcca tattcaattc
     4681 ggcaatcgtt ttaaccaaca tcagcgcaat gggtagcagt atggtgaata acgttgcgcc
     4741 taatgacggt agtgtttttt catcgcgaac tttgagatct gcaaactctg tgggtacagg
     4801 tttaaagggc agtcgttgac ccagaaattt aaggaaaagt gggccaccga tcagtgatgc
     4861 catcagccca accagcaaac cgtagacgat caccgaaccg atatctgcgc ccagcttatt
     4921 ggcaacatat aaagcagccg gatgtggagg aaccacgcag tgcactgcca tcaatgcggt
     4981 acatagcgga atggcaagct ttaataatga ggtattggtt tttttggcaa ttgaaaaagc
     5041 cagaggaatc aatagcacga cgcccacttc aacaaacagc gtgatgccac aaatcaggcc
     5101 aaccagcacc ataatgacat cagctgaaag ccagcggcag cgttgaagtg tcagaccaat
     5161 tctttctgcg gccccggata cttccatcat ttttcccagt atcgtgccaa ggccgataac
     5221 cgctgcgagg aaccccagcg ttccgccaat tccactttca atagcattta ccatatccag
     5281 tggccccatg cccatcatcg ttcccacgaa gaagctggcc agcaacagcg ccagaaacgg
     5341 gtggaatttg aacttcacga tggtcagtac aattaacacg atgctgataa gcagcgtgct
     5401 cacaacccag atttgagagt gcatatctca ccttcccctg tgattaacct gatgatattg
     5461 gacaaaaaaa caacgcaggc tgacaaacga taaaatttgc ctttcacatg aggtaaattg
     5521 catcaatgga gtgatttaca tctaaaaatg aaggtgaatt gagatatggt tcactttagc
     5581 tcatctcata gctgaaattt tgccgctatt tttttacact taagcgaaaa atgaggtgat
     5641 tatggaaccc cttcgtgaaa taagaaatcg gctgcttaac ggctggcaat tatcaaaaat
     5701 gcatactttt gaagtggctg ccaggcatca gtccttcgcc ctggcggcag aggaattgtc
     5761 gctgagcccc agtgcggtaa gtcaccgtat caatcagctg gaagaagaat tgggcattca
     5821 gttgtttgtt cgttcccatc gcaaagtgga attaacgcac gaggggaaac gtgtttattg
     5881 ggcgctaaaa tcgtcgctgg ataccctgaa ccaagaaatt cttgatatca aaaatcagga
     5941 gttatcggga acgttaactc tgtattcccg gccctctatc gcccaatgct ggttggtgcc
     6001 cgcattaggt gactttacac gccggtatcc gtctatttcg ctcaccgtgc tcactggtaa
     6061 tgacaacgtc aatctgcaac gtgccggaat cgatttggcg atctactttg atgatgcgcc
     6121 gtcagcgcaa ctgactcatc actttctgat ggatgaagaa atcctgccag tatgtagccc
     6181 ggaatacgct caaagacatg ctttaaccaa cacggtaatt aacctgtgtc actgtacgtt
     6241 gctccatgac agacaggcat ggagcaacga ctccggtacg gatgaatggc atagttgggc
     6301 gcaacattat gcggttaatt tgccgacatc ttctggaatt ggctttgatc gttctgattt
     6361 agctgttatt gccgcgatga atcatattgg ggtggcgatg ggaagaaaac gcctggtaca
     6421 aaaaaggctt gccagtggtg agctcgtcgc gccgtttggc gatatgacgg tgaaatgcca
     6481 tcagcattat tacatcacca cattaccggg caggcagtgg ccaaaaattg aagcgtttat
     6541 tacctggcta agagaacagg taaaaaccac ttcataatga ataaaaccac gagttcctgg
     6601 tgtgccttat tcggcctgct agttcgaatt taatttacgg tttttatgtg gttttaggga
     6661 taaacacaag gatggtgtcc cctgcaggaa tcgaacctgc aattagccct taggaggggc
     6721 tcgttatatc catttaacta agaggacaat gcggcatgag tatacccgct aatggactgc
     6781 ggggtaagta cgctgccgct cgattgctta aaccctcgcc atttatgctg ggtttttatc
     6841 atttttctta atgttttccg cacgttctgc tttttggcgt gcttctgctt tacgcttgtt
     6901 gctcatgtcg ttacgaatct gtgcatgact cattaacgcg aagataaagg tgccgccgca
     6961 gatgttcccc gctaaagtag gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt
     7021 gccgttaaac accagataga ggatttcaac agaaccgaca acgatgtggg tggtgtcacc
     7081 cagtgcaata agccaggtca tcaatataat caccacaatc tttgccgcac ccgcagcagg
     7141 aaacatccaa accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
     7201 gctgggggtg ttcttcatca catccatgcc gattttgaca aatgcatcgc gagtttcttc
     7261 attgaagata ggcatatatt caaatgccca tgcagcaata cctgtcccga gaatattacc
     7321 cagcagcacg acgccccata atcgcataag taagccgacg ttgctcattg tcggtttttg
     7381 catgacgggt agtaccgcag tcacggtgtt ttcggtaaat aattgctggc gggccataat
     7441 gacgataata aaaccaaagg tataaccgag attctccagt aagaagctgc ctggcactcc
     7501 ttcccgttcg acatgaaata tcccttttgc cagtagcgaa gcgcccatcg acagacccgc
     7561 cgcaatggct gaccacagta gcgccattgc gtcgcgttcc agctcttttt caccatcctg
     7621 gcggatatgc tcatgaattg ccatcgcccg ggaggggagt cggtcttcat ctatttctat
     7681 ttttttgccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
     7741 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
     7801 tttttgctcg caacaggcgg gagttactct gaaaatgtag aaaaggctgc gtttgccttt
     7861 ttctgtttct atagaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
     7921 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
     7981 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
     8041 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     8101 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg ttcaaccgca
     8161 ccatgtacaa cttcaacttc aatgtattag acccgtatat tgttcgaccg gtcgctgtcg
     8221 cctggcgtga ttatgttccg caaccggcgc gtaacggttt gagcaacttt actggcaacc
     8281 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     8341 tccactttac ccgctttttc ctgaacacca ttttggggat gggcggtttt attgatgttg
     8401 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     8461 gtcattatgg cgtgggttat gggccttacg tccagttacc gttctacggt agcttcacgc
     8521 tgcgtgatga cggtggtgat atggcggatg gtctttaccc ggttctttcc tggctgacct
     8581 ggccgatgtc tgtgggtaaa tggacgcttg aagggatcga aacccgcgct cagctgctgg
     8641 attccgatgg tctgctgcgt cagtcgtccg atccttatat tatggtgcgc gaagcgtact
     8701 tccagcgtca tgatttcatc gctaatggcg gcgaactcaa accgcaggaa aatccgaacg
     8761 cgcaagcgat tcaggatgat ttaaaagata tcgattctga ataagaaaca aataaaaaag
     8821 gtgagtcgca atactcacct ttttt
//
LOCUS       DAZFAH010000174         8783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFAH010000174 DAZFAH010000000
VERSION     DAZFAH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.176"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..592
                     /gene="ftnB"
                     /locus_tag="ACHJWB_003842"
     CDS             89..592
                     /gene="ftnB"
                     /locus_tag="ACHJWB_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4315400.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(671..922)
                     /gene="yecJ"
                     /locus_tag="ACHJWB_003843"
     CDS             complement(671..922)
                     /gene="yecJ"
                     /locus_tag="ACHJWB_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4315401.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1037..1123)
                     /gene="azuC"
                     /locus_tag="ACHJWB_003844"
     CDS             complement(1037..1123)
                     /gene="azuC"
                     /locus_tag="ACHJWB_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4315402.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1387..1710
                     /gene="yecR"
                     /locus_tag="ACHJWB_003845"
     CDS             1387..1710
                     /gene="yecR"
                     /locus_tag="ACHJWB_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4315403.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLCAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1881..2378
                     /gene="ftnA"
                     /locus_tag="ACHJWB_003846"
     CDS             1881..2378
                     /gene="ftnA"
                     /locus_tag="ACHJWB_003846"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4315404.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2415..2654)
                     /gene="yecH"
                     /locus_tag="ACHJWB_003847"
     CDS             complement(2415..2654)
                     /gene="yecH"
                     /locus_tag="ACHJWB_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4315405.1"
                     /translation="MDYIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELMAFLAAKGKFTPSEDGFSTDQSKICRH"
     gene            2845..4056
                     /gene="tyrP"
                     /locus_tag="ACHJWB_003848"
     CDS             2845..4056
                     /gene="tyrP"
                     /locus_tag="ACHJWB_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4315406.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFTLFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4118..4783)
                     /gene="yecA"
                     /locus_tag="ACHJWB_003849"
     CDS             complement(4118..4783)
                     /gene="yecA"
                     /locus_tag="ACHJWB_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4315407.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQKKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(4979..5065)
                     /locus_tag="ACHJWB_003850"
     tRNA            complement(4979..5065)
                     /locus_tag="ACHJWB_003850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5029..5031),aa:Leu,seq:taa)
     gene            complement(5078..5151)
                     /locus_tag="ACHJWB_003851"
     tRNA            complement(5078..5151)
                     /locus_tag="ACHJWB_003851"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5117..5119),aa:Cys,seq:gca)
     gene            complement(5205..5280)
                     /locus_tag="ACHJWB_003852"
     tRNA            complement(5205..5280)
                     /locus_tag="ACHJWB_003852"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5245..5247),aa:Gly,seq:gcc)
     gene            complement(5432..5980)
                     /gene="pgsA"
                     /locus_tag="ACHJWB_003853"
     CDS             complement(5432..5980)
                     /gene="pgsA"
                     /locus_tag="ACHJWB_003853"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4315408.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6037..7869)
                     /gene="uvrC"
                     /locus_tag="ACHJWB_003854"
     CDS             complement(6037..7869)
                     /gene="uvrC"
                     /locus_tag="ACHJWB_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4315409.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDTKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7866..8522)
                     /gene="uvrY"
                     /locus_tag="ACHJWB_003855"
     CDS             complement(7866..8522)
                     /gene="uvrY"
                     /locus_tag="ACHJWB_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4315410.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2161 a         2222 c         2055 g         2345 t
ORIGIN      
        1 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
       61 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
      121 ctctcaaatg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
      181 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
      241 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
      301 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
      361 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
      421 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
      481 cctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgctg gtatgtgccc
      541 agtgcagacc gaccaacatg ttctgaatgt cgtgtcgcat cagctgcatt gatcatacat
      601 cggcgctaat gcattgcgcc gatgaagttt ttgagaaacc gctgcctcat ctttttgaag
      661 cagcggtttt ttaatgggat tcgccttgtg gggtaaactt gagttcaatc agcgcgatgg
      721 ctttttggat tgcccgcatg gtgaccgggt ctgcggccgc gggatggtta ctaaagtcga
      781 tattcttcag ctggctggac attttttcgc gaacttcaac gggcgcgatt acatcgagaa
      841 catccagaat ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat
      901 cggcattcag cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta
      961 gcggcttcac atcttcagcg atagtcacat ccaccgtcat caggagacaa aaaaacctgc
     1021 cggagcaggt tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc
     1081 aatagttatt gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgagcat
     1141 ttgttataca ggtgttattg tctttgcact tataatatga caaccatcac aaaaatcaat
     1201 ctttatgtga tgtgaatcac ataaataccc cctttaatgt tataaaaatg ataatcaaaa
     1261 agcagccccc ccatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt
     1321 ttccggccat tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc
     1381 tggaccatga gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt
     1441 actcgccagg cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat
     1501 gaccaggctt ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat
     1561 cgcgcatgcc agcaggaagg ctatacccac gcgatcccct ttggtcagcc agtaggcaac
     1621 tgtagccttt gtgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc
     1681 catcattccg ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg
     1741 attaaagaaa tagcggtaat gcgttttatt cccattcgta tttttaataa ttgaagttta
     1801 tattttacct tttgcaaata ataaaataac aaattatagt gacgccacag caacaaatat
     1861 aacttttgtg gaacactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga
     1921 tgaacctgga actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc
     1981 ataccttcga aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata
     2041 tgcagcgtct gtttgattac ctgactgata ccggcaattt accgcgtatt aataccgttg
     2101 aatctccgtt tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg
     2161 aacaattaat cacccagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact
     2221 acccaacatt taatttcctg cagtggtatg tttctgagca gcatgaagaa gagaaactgt
     2281 tcaaatcgat tattgataaa ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta
     2341 tcgacaaaga actctctacc ctcgacacac aaaactaatg ctctccggcg gcaggtttct
     2401 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accgtcttca
     2461 gaaggagtaa atttgccttt tgctgccaga aacgccatca gctctcccgc tgtcatccct
     2521 tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
     2581 agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg
     2641 tgaatatagt ccatcgttcc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa
     2701 gactttgcgc cagaacagaa ctgaatgctt tttattgtac atttatattt acaccatatg
     2761 taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata
     2821 aagatcgtca ggacagaaga aagcgtgaaa aacagaactc tgggaagtgt ttttatcgtg
     2881 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt
     2941 tttagcgtca cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     3001 ttactgctgg aggtgtacca gcatgttccg gcagataccg gtcttggcac gctggcaaaa
     3061 cgctatctgg gacgctacgg tcagtggctg acgggcttca gtatgatgtt cttaatgtat
     3121 gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     3181 tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt
     3241 ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc
     3301 aaaattattt ttctggtggt gatgctggta ttgctgctac cgcatattca caaagtgaat
     3361 cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt gatttttacc
     3421 tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacgttcgt
     3481 aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg
     3541 caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     3601 gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     3661 ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta
     3721 ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg tggacggttg
     3781 caaactggtg caattacctt tctgccgccg ttggcgttta cactgtttta tccacgagga
     3841 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     3901 tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtgaaaggt
     3961 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt
     4021 ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt
     4081 gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag cactgcttaa
     4141 atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga
     4201 tcggttgctg aacagctttt ttctgcggat gcgccatcca gtatgcatgt aaatcaagcg
     4261 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc
     4321 gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat
     4381 cgggcaatgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct
     4441 caacaatcgt cagctcgctg ccatcgactt ctcgcaagcc gaataacggc tcaaactgct
     4501 ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc aggttcataa
     4561 agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg
     4621 caaccagcca ctgttccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     4681 cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     4741 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga
     4801 tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg
     4861 ctttgccggt gattaaaaat taaggagggt gtaacgacaa gtttcaggca caaaaaaacc
     4921 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttatgcttat ctttcccatg
     4981 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     5041 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     5101 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     5161 aggcctttca gccagacatc cgctgacgcc gatgtctttt aaactggagc gggaaacgag
     5221 actcaaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
     5281 attcatcaag caatcagtta atcacttgat tttattatcg tctggcaatc agtgccgccg
     5341 ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt tcaccacttt
     5401 tgatcgtttg ctgaaaattg cgccgaaacg atcactgatc aagcaaatct gcacgcgcag
     5461 cgctcaaata ttgcaacatt gaccacagag tcagtaccgc agccacaaag aaaagtgcaa
     5521 taccggcgta ctcaacccaa atgttcggac gccacagcag ccaggccaac gccaccatct
     5581 gggcagtggt tttcactttc ccaatccagg agacggccac gctactgcgt ttacccaact
     5641 ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc gttgccgccg
     5701 gtaaggtcac ccaccagctg tgataatgct cggttaccag caccatggcg atagccacga
     5761 ggactttatc tgctacaggg tcaaggaaag caccaaaccg ggtactctgg ttccagcggc
     5821 gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg
     5881 caaacggcga ccaggtgaca ggcagataaa agaccaatac aaagaatggg ataaggatga
     5941 cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt aactatctgt
     6001 tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg accagaagat
     6061 cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga cgctggcgtt
     6121 acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccgacgtt ttggcccgac
     6181 gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt tacggtgccc
     6241 gccaatcgcg tgatcgtgtg attcatcgcg aatatgctgg ataacatgca gcgcgggaga
     6301 atctggcggc aaactgaatc cctcaccttc cggctcaaag aacagtgttt ccagcccagc
     6361 cttacgatct gctcctttgg caacgccaag tagcagtgga tgatttttat cccatgagac
     6421 atccagttcg gcaaagacat tttttgcctg cgcaagctgg cctttgccgc cgtcgataag
     6481 gatcacatcc gggatcttag tgtcgtcaat ggctttacca taacgccgac gcagcacctg
     6541 attcatcgcc gcataatcat cgcccggcgt gatgccagta atgttatagc gccgatactc
     6601 cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt gttcacccat
     6661 ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca acacgctggc
     6721 aagtgctgta agccgctgat gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt
     6781 cgccgcattg gtgcgcgcga gtttcaggta acgcgcccta tcgccacgag gtttggtttg
     6841 aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg ttttatcgct
     6901 aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc cttgtaaata
     6961 gaactgtcct acgaaggttt ccaccacctc gctcagttcc gtaccgccag gcactttcgg
     7021 gaaatagctg cggctgccaa gcactttgcc ctgacgaatg aacaatacgt ggacacaagc
     7081 catgcccgca tcgaacgcca caccaataac gtcaaggtcg tcgccggtat tggaaacaaa
     7141 ctgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacgtg ccgcttcttc
     7201 aaactccaga ttctgactgg cagtttccat acggctaatc agttgcgtaa gcacctgatc
     7261 atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt cttcttcact
     7321 caccagtcct tcaacgcacg gtcccagaca gcgccctatc tgatattgca gacacggacg
     7381 cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct tttgcagtaa
     7441 cgccagtgtt tcacgtacag catagccatt cgggaacggg ccgaaatatt cacctttggc
     7501 atgcttcgca ccacgatgca tcgccagacg cgggtgggta tcaccactca ggaagataaa
     7561 aggatatgat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga gtttgatgta
     7621 gttgtgttcc agcaacagcg cttcggtttc tgtgtgagta accgttacat caatttgctg
     7681 gatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga aatagctgga
     7741 aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac cagcatcgta
     7801 catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg cgtcaaactg
     7861 atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat tgccaggtga
     7921 gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg gtagctgttc
     7981 accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg gcccttggtg
     8041 atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct ttctgttttt
     8101 tctggttcga tctggcttaa cgccatttgt tgagcgatgt cagaagcaat gtaacgctgc
     8161 cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc tttgctgagg
     8221 tagcccgcag caccggcctg catgactttc gctggtaaag ggttttctgt atggacggta
     8281 agcatgatga ttttgacatc ggctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg
     8341 ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgc ccgacaccac
     8401 ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt tatatcttcc
     8461 agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag aacgttgatc
     8521 aaaggaatat ctccagaaat agggataacg ctactgatag ttagtcattc gtatattagc
     8581 ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt ctctcgtttt
     8641 tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggcttc cgggatcgtt
     8701 tcacagtaag cccggcgatg aaatgtgaca aaaattgact ttattcagca aaaatgaaaa
     8761 tcagcccgct gaatatgtaa cat
//
LOCUS       DAZFAH010000175         8696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFAH010000175 DAZFAH010000000
VERSION     DAZFAH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.177"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHJWB_003856"
     CDS             complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHJWB_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4315411.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            1761..1836
                     /locus_tag="ACHJWB_003857"
     tRNA            1761..1836
                     /locus_tag="ACHJWB_003857"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Asn,seq:gtt)
     gene            complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHJWB_003858"
     CDS             complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHJWB_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO4315412.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2926..3843)
                     /gene="nac"
                     /locus_tag="ACHJWB_003859"
     CDS             complement(2926..3843)
                     /gene="nac"
                     /locus_tag="ACHJWB_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4315413.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4170..4245
                     /locus_tag="ACHJWB_003860"
     tRNA            4170..4245
                     /locus_tag="ACHJWB_003860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4203..4205,aa:Asn,seq:gtt)
     gene            complement(4301..5236)
                     /gene="ldtA"
                     /locus_tag="ACHJWB_003861"
     CDS             complement(4301..5236)
                     /gene="ldtA"
                     /locus_tag="ACHJWB_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4315414.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5298..6377)
                     /gene="cobT"
                     /locus_tag="ACHJWB_003862"
     CDS             complement(5298..6377)
                     /gene="cobT"
                     /locus_tag="ACHJWB_003862"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4315415.1"
                     /translation="MQTLADLLNTIPAIDPAVMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6389..7132)
                     /gene="cobS"
                     /locus_tag="ACHJWB_003863"
     CDS             complement(6389..7132)
                     /gene="cobS"
                     /locus_tag="ACHJWB_003863"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4315416.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7129..7674)
                     /gene="cobU"
                     /locus_tag="ACHJWB_003864"
     CDS             complement(7129..7674)
                     /gene="cobU"
                     /locus_tag="ACHJWB_003864"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4315417.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7826..7999)
                     /locus_tag="ACHJWB_003865"
                     /pseudo
     CDS             complement(7826..7999)
                     /locus_tag="ACHJWB_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7978..8166)
                     /locus_tag="ACHJWB_003866"
                     /pseudo
     CDS             complement(7978..8166)
                     /locus_tag="ACHJWB_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2419 a         2323 c         1868 g         2086 t
ORIGIN      
        1 aactacagag gaatcgtgag agcgaggcga atattagcga tgcccaacca caatgtcaaa
       61 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
      121 acacagctgg ctttttttca cacttttgcg gttcgggtcg agggtatttc catagccaac
      181 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
      241 ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa
      301 cccggcaacc ccacatactc aacatagaaa cccacatagc gtaacgggcg tcacgagccc
      361 ccttaaatcc agcgggcagc acccatgagg cggaccaaat aggcataaat aaagcattta
      421 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
      481 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggaaagcc
      541 agaacacatg ccgcaactga atctctgctt gcgctatctg tcctacaccc aaccttcggc
      601 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
      661 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
      721 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgcggga ataccaatcc
      781 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
      841 ttagcgcagg attaaaacca atcgccaaca cccacaaaat tgcaactgcg ccaatataac
      901 gagaaatggt taaacccaac ccagccccga caaatcccag tcccggccag gagaaaaggc
      961 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta
     1021 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaattaga gtgatggcag
     1081 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtgct ttaacttctg
     1141 tcgtggcatc acccgcgacg aaatcaataa tttgctcgcc aaaatgatga ataagcgttg
     1201 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     1261 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     1321 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     1381 catcttttcc cagccagctg accagaaaag tgctcagaac ccccatcaac aggacacagg
     1441 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     1501 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     1561 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     1621 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     1681 gaaacggcaa acagttggga aaattaaaaa tcgggtttga caaaagattt ttcgccgtta
     1741 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     1801 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat
     1861 agcgaacttt ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctgcc
     1921 gcttgatatc ctcaactgaa agccctgcat tacaaagttc cagaaagcgc cagacatagt
     1981 tacgctgaag ttgtcctcgc ttcagtccca accagacagt attagcatca aaaagatgcc
     2041 gcgtatccag gcggattaaa ttcttttcct cttgttcacc gctggattgt tcggcaacta
     2101 ttccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta
     2161 atacaatatc tgccagcaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg
     2221 taatcccctg tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg
     2281 taatttgcgt caagggatga tcgagtggaa caagcaaact atggtgccaa cgaaaccacg
     2341 ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt
     2401 caccattatg caacaacgtc gcaatttcct gtggcgtccc ctggattagc tcgagccgaa
     2461 cctccgggaa aagttcgcga aaagatttaa tgacctctgg caaactataa cgtgcctgag
     2521 tatgcgtcgt tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc
     2581 gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg
     2641 gttcagtcat gcccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat
     2701 cctccagttc ccgaatatga cggctgacgc cggactgtga ggtaaaaagc atattcgcaa
     2761 cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat
     2821 tcacggtaaa ctccgggcag ttcagatttt ccgttattgt taaagtctaa tgcccggcat
     2881 aacaaataat aaaaacccgc atcttattcc atcccgatat aacgcttagc tcaccaattg
     2941 ccactgcctt ttttccatca ctggagaact catcactgac attaacaact ctttcactgc
     3001 ctgtgcctgt ggtgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
     3061 ggaaggtgtg gtaatgcgtg acatccaccc gtttactgcg ccacataacg aacgcgcggc
     3121 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
     3181 agactcaatt tcaccaatga cttttgccgt gagtcgccgt agggaaaaag cctcatcaac
     3241 acgaagtcta acagcactgt aatcactggg aagaaagagg ttcatttgcg caatagcatt
     3301 aacatcaacg ctttgccccg gacaatcttg agtccctacc aggaaaagat cttctttcag
     3361 taaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
     3421 ttggtgattt atcagttttt cgttaagcac tgcaccgcta ttttcatgaa gatagataac
     3481 gacctccggg aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
     3541 ggttcctggc gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
     3601 cgccagttgg gcctgctcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
     3661 tccggcgtca gttggcgtaa cgccccgttt tgtgcgaatc aaaagttgtt gattaaactc
     3721 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
     3781 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
     3841 catgttgcct ccggttttta agagtcggcc caagtgccgc cattacttac aaccagaatg
     3901 caagatgctt gccagttcta ttttggcgtt gatgcgcaag ctaaccaact gtcaaataag
     3961 agattatgat agattcgtca ttggctcttt ttatctgcag tcgcattcac ctgccctata
     4021 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     4081 ttgaaattgg cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     4141 gccattaata tgtgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     4201 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     4261 gctttcgagc aggcttttta ctttctaatt atcaacgctc ttaaaacatc tgtcttgaac
     4321 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     4381 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     4441 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     4501 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     4561 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     4621 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     4681 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     4741 aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc caggttggtg
     4801 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     4861 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     4921 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     4981 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     5041 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     5101 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     5161 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaaaaatat
     5221 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     5281 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     5341 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     5401 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     5461 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     5521 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     5581 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     5641 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     5701 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     5761 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     5821 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     5881 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     5941 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     6001 agcccgggga taggctcagc actatcaata ccaacatcaa ctacgtggac gttagcgccc
     6061 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     6121 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     6181 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcaaccccgg cattcccgcc
     6241 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtca
     6301 atatgccgtt gtgcacgcga cataacggca ggatcgatag cagggatcgt attcagtaaa
     6361 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     6421 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     6481 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     6541 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     6601 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
     6661 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     6721 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     6781 tcaatgccag accgccatgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     6841 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     6901 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     6961 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     7021 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     7081 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttactc atttgatttt
     7141 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
     7201 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     7261 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
     7321 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     7381 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
     7441 cacaacctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     7501 cactgtgcgc cagtgctccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     7561 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaagagtccc caataagcgc
     7621 ctctgcgtgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     7681 tcctgattca aatgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     7741 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     7801 caatccatta cgaaataaga tggcattacg gaatagccag actttctcgt gttaacgtcc
     7861 gacaaaatgt gtcattgaca gcggtttctg tggattgaca catttcattc agataatgaa
     7921 ttaatgctac tgccggacca accagcaaac gggcattgtg ttctgaaatc caggctattg
     7981 attcaacgtc agcgacagat ctgcgctgta aaagaacttg taaaacaacg caatagaatt
     8041 atccggaatg gtggcgacta tgcactagaa caccacaatt tcgctctctc ggtaaggcac
     8101 tgtcacagca ttcaacagaa tgtgacttgc cagatttatt agcgccacca gatgtttaac
     8161 cgggtaacca cgacccagtc ggggatatat tccatcttct cgctgacatc atcaagaata
     8221 aaaaggttta tcacactcag agcaacgttc ttcagcaggc gggataattc ttcaacattt
     8281 aaaagatgtg ttattaaagg catacaaact ggacgtattg tattttcttt tggtggttga
     8341 cctaaggtag cagtttatcc tgatgcgctg agatttctgt aatatctacg tcaacattct
     8401 cctcgaatag aaaatgcagc cccggcaagt gattcacatt ttttaacaaa acgttgttag
     8461 cgtgccattt tcagaagctc tccaagaaag cgaattcttt tctcaaattc tgcattctca
     8521 gacacagctt tctactccat ggcaatggcc caaatacgaa gttgctcagg atcattagtg
     8581 gtgctgagaa gttaaataac catgcggagt accactcgtc atcacgtatt tatatatcat
     8641 gaagctattg ttgtgttttg tacatcagta gatgtattct gatatactcc ttttgc
//
LOCUS       DAZFAH010000176         8657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFAH010000176 DAZFAH010000000
VERSION     DAZFAH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.178"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(270..533)
                     /gene="rpsT"
                     /locus_tag="ACHJWB_003867"
     CDS             complement(270..533)
                     /gene="rpsT"
                     /locus_tag="ACHJWB_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4315418.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            636..854
                     /locus_tag="ACHJWB_003868"
     CDS             636..854
                     /locus_tag="ACHJWB_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4315419.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            862..1803
                     /gene="ribF"
                     /locus_tag="ACHJWB_003869"
     CDS             862..1803
                     /gene="ribF"
                     /locus_tag="ACHJWB_003869"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4315420.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1846..4662
                     /gene="ileS"
                     /locus_tag="ACHJWB_003870"
     CDS             1846..4662
                     /gene="ileS"
                     /locus_tag="ACHJWB_003870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4315421.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRVQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4662..5156
                     /gene="lspA"
                     /locus_tag="ACHJWB_003871"
     CDS             4662..5156
                     /gene="lspA"
                     /locus_tag="ACHJWB_003871"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4315422.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5281..5730
                     /gene="fkpB"
                     /locus_tag="ACHJWB_003872"
     CDS             5281..5730
                     /gene="fkpB"
                     /locus_tag="ACHJWB_003872"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4315423.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5732..6682
                     /gene="ispH"
                     /locus_tag="ACHJWB_003873"
                     /gene_synonym="lytB"
     CDS             5732..6682
                     /gene="ispH"
                     /locus_tag="ACHJWB_003873"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4315424.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6748..7662
                     /gene="rihC"
                     /locus_tag="ACHJWB_003874"
     CDS             6748..7662
                     /gene="rihC"
                     /locus_tag="ACHJWB_003874"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4315425.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7829..8650
                     /gene="dapB"
                     /locus_tag="ACHJWB_003875"
     CDS             7829..8650
                     /gene="dapB"
                     /locus_tag="ACHJWB_003875"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4315426.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1968 a         2167 c         2465 g         2057 t
ORIGIN      
        1 ttaatacctc aatgtgtatc acaatatcca cattctttgt aggggagtct ggagattgag
       61 tagatattct tgttcagaat gtatcagccg atggttctac gattcttaag ccacgaagag
      121 ttcagaaagt gtaacggcat gtctgcaaaa gccagcatct tttgactatc tggcaaccgg
      181 tagtgtgttc tctcacgcat cataaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      241 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      301 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      361 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      421 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      481 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      541 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      601 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      661 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      721 cagcaggcgg tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc
      781 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      841 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      901 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
      961 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1021 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1081 cggctgcggg aaaaattgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgta
     1141 cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg
     1201 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1261 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1321 acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca ccgccgtgcg tcaggccctt
     1381 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1441 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1501 ctgcgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1561 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1621 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1681 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1741 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1801 taagcctgtt atgtaatcaa accgaaatac ggaaccgaga atctgatgag tgactataaa
     1861 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1921 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     1981 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2041 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2101 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2161 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2221 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2281 gtgctgggcg actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc
     2341 atccgcgcgc tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt
     2401 cactggtgcg ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa
     2461 acttctccgt ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa
     2521 tttgccgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2581 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2641 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2701 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc
     2761 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2821 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2881 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     2941 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3001 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3061 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg
     3121 gatcagaaag gtctgcgtgt gcagtcactg aaagagatca aaggcgtgca gtggatcccg
     3181 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3241 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3301 catccgcgta ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc
     3361 caggcgtggt gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg
     3421 aaagtgccgg acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt
     3481 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3541 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3601 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3661 tccatcggca ataccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3721 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3781 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3841 ctgaacggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3901 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     3961 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4021 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt
     4081 cgtagctgcc agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca
     4141 atcctctcct tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac
     4201 gtcttcaccg gtgagtggta cgaaggcctg tttggcctgg cagacagtga agcgatgaac
     4261 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaataaagt cattgagcaa
     4321 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4381 ccggaactgt cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4441 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4501 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4561 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4621 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4681 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4741 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4801 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggtt
     4861 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgatccta gcagtgatga
     4921 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     4981 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5041 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5101 gtgcggcact gattgtgctg gaaggttttt taccttctaa agcgaaaaaa caataataaa
     5161 ccctgccgga tgcgatgctg acgcatctta tccggcctac agattgctgc gaaatcgtag
     5221 gccggataag gcgtttacgc cgcatccggc aaaaatcctt aaatataaga gcaaacctgc
     5281 atgtctgaat ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac
     5341 gatggcacca ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt
     5401 gatgcttctc tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa
     5461 accaccttct cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag
     5521 tacttctccc gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt
     5581 tttaccgcaa tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc
     5641 attaccgttg atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg
     5701 ctggaaatcg atccggcact ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg
     5761 tttttgtgcc ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg
     5821 cgcaccgata tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg
     5881 tgagcgtggg gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat
     5941 tttctccgca cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagtc gcgatttgac
     6001 ggtatttgac gcgacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag
     6061 tcgccgtggc gaagaatcta ttctcatcgg tcacgccggg cacccggaag tggaagggac
     6121 aatgggccag tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt
     6181 gtggaaactg acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc
     6241 ggtggatgac acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg
     6301 tccgcgcaaa gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct
     6361 ggcagaacag gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg
     6421 tctggcggag ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga
     6481 catccaggaa gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc
     6541 tccggatatt ctggtgcaga atgtggtggc acgtttgcag cagctgggcg gtggtgaagc
     6601 cattccgctg gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt
     6661 cgatattcgt gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt
     6721 gttaccggca ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc
     6781 ggcattgacg atgccgtcgc cattgccgcc gcgatttttg cacccgaact cgacctgcaa
     6841 ctgatgacca ccgtcgcggg taatgtctcg gttgagaaaa ctacccgcaa tgccctgcaa
     6901 ctgctgcatt tctggaatgc ggagattccg ctcgcccaag gggccgctgt gccgctggta
     6961 cgcgcaccgc gtgatgcggc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt
     7021 gttgagcaca accgaaagcc gctcgggata ccggcgtttc tggcgattcg ggatgccctg
     7081 atgcgtgcac cagagcctgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg
     7141 ttactttcac aatgcccgga atgcaagccg tatattcgcc gtctggtgat catgggtggt
     7201 tctgccggac gcggcaactg tacgccaaac gccgagttta atatcgctgc cgatccagaa
     7261 gcggctgcct gtgtcttccg tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc
     7321 aatcaggcaa tattaacccc tgactatctc gctactttgc cggagttgaa tcgcaccgga
     7381 aaaatgcttc atgcactgtt tagccactac cgtagcggca gtatgcaaag cggtctgcga
     7441 atgcacgatc tctgcgccat cgcctggctg gtgcgcccgg acttattcac cctcaagccg
     7501 tgttttgtcg cggtagaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc
     7561 gacggttgcc tgggcaaacc agccaatgta aaggtggcat tggatctgga tgtgaaaggc
     7621 ttccagcagt gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg
     7681 catgcagtca ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt
     7741 tctaattatc agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt
     7801 ctatgcaaat taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc
     7861 gggagccggg gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt
     7921 gcagctgggc gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga
     7981 gctggctggt gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga
     8041 tgattttgat gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt
     8101 ttgtcgccag catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa
     8161 acaggcgatt cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt
     8221 tggcgttaac gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac
     8281 cgatatcgaa attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc
     8341 actggcaatg ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt
     8401 ctacagtcgt gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt
     8461 gcgtgcaggt gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct
     8521 ggagatcacc cataaggcgt ccagccgcat gacatttgct aacggcgcgg taagatcggc
     8581 tttgtggttg agtggtaagg aaagtggtct ttttgatatg cgagatgtgc ttgatctcaa
     8641 tagtttgtaa ccacaaa
//
LOCUS       DAZFAH010000177         8638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFAH010000177 DAZFAH010000000
VERSION     DAZFAH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.179"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..545
                     /gene="slyA"
                     /locus_tag="ACHJWB_003876"
     CDS             111..545
                     /gene="slyA"
                     /locus_tag="ACHJWB_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4315427.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(592..1059)
                     /gene="slyB"
                     /locus_tag="ACHJWB_003877"
     CDS             complement(592..1059)
                     /gene="slyB"
                     /locus_tag="ACHJWB_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4315428.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1333..2442
                     /gene="anmK"
                     /locus_tag="ACHJWB_003878"
     CDS             1333..2442
                     /gene="anmK"
                     /locus_tag="ACHJWB_003878"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4315429.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGRRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2540..2869
                     /gene="mliC"
                     /locus_tag="ACHJWB_003879"
     CDS             2540..2869
                     /gene="mliC"
                     /locus_tag="ACHJWB_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4315430.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2928..3584
                     /gene="pdxH"
                     /locus_tag="ACHJWB_003880"
     CDS             2928..3584
                     /gene="pdxH"
                     /locus_tag="ACHJWB_003880"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4315431.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3713..4987
                     /gene="tyrS"
                     /locus_tag="ACHJWB_003881"
     CDS             3713..4987
                     /gene="tyrS"
                     /locus_tag="ACHJWB_003881"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4315432.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5046..5909
                     /gene="pdxY"
                     /locus_tag="ACHJWB_003882"
     CDS             5046..5909
                     /gene="pdxY"
                     /locus_tag="ACHJWB_003882"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO4315433.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5953..6558)
                     /gene="gstA"
                     /locus_tag="ACHJWB_003883"
     CDS             complement(5953..6558)
                     /gene="gstA"
                     /locus_tag="ACHJWB_003883"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4315434.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6664..8166)
                     /gene="dtpA"
                     /locus_tag="ACHJWB_003884"
     CDS             complement(6664..8166)
                     /gene="dtpA"
                     /locus_tag="ACHJWB_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4315435.1"
                     /translation="MSTANPKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         2259 a         2116 c         2182 g         2081 t
ORIGIN      
        1 tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt gctgcacact
       61 attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa ttggaatcgc
      121 cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata gaccatcgcc
      181 tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc catcagttac
      241 ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag ccatcactgg
      301 tccgtactct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact tgtgccagcg
      361 atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccactgatc agcgaaatgg
      421 aagctgttat taacaaaacc cgcgcggaaa tattacatgg catctccgca gaggaactgg
      481 agcaactgat tacgctcatc gcaaaacttg agcataatat cattgagtta caggccaaag
      541 ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta tttagcgcgg
      601 agaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg gagagaaacg
      661 agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac gaatttccag
      721 ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccagcta cgccacctgc
      781 aacagcgcct gctgcagtag ccagagaacg cccggttccg ccaccaacag tattccccag
      841 gaaaccacca agaacagcac cgccaattgc accgataacg ttggaatcat caccgccctg
      901 aatctgtacc ggacgtacgt taacgatggt gccatagctg acattctgta cttgtttcgc
      961 ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga
     1021 cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca
     1081 tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt
     1141 acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa
     1201 aacattcatg atgattattg atttgaagat aatgttttac taccaacggt aaaaatcggt
     1261 cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg tcagattgaa
     1321 aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac cagccttgat
     1381 ggtgttgatg ttgtgttggc gacaatagat gaacaccggg tcgcacagct ggcaagcttg
     1441 agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca gggccagcag
     1501 cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt tgctgatgcg
     1561 gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc gatcggctgt
     1621 cacggtcaaa ccgtctggca tgaaccgacg ggcgtggaac cacacacttt acagattggc
     1681 gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt tcgccgtcgc
     1741 gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca tgccttgctg
     1801 gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa tctgtcactg
     1861 ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat gctgatggat
     1921 gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg ggcacgggcg
     1981 ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt ctcgcaacct
     2041 gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg ccatttacgc
     2101 cattttccgg gtgttgatcc ccgtgatgtt caggccacac tggcagaact caccgccgtg
     2161 accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt atgtggtgga
     2221 ggtcgtcgta atccgctact catggcgcgt ctggcggcat tactgccagg cacagaagtc
     2281 accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc tttcgcctgg
     2341 cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac tggcgcaagc
     2401 caggagacgg tactgggggc tattttccct gctaacccgt gatatcagag ttaactgaat
     2461 ttttcttacc gcgtctgccg ttacacttgg aacattaggg agggcgtatt gccctccaga
     2521 ccaggaaagt cttcgggaga tgtctatgaa aaaactgtta ctgatctgtt tgccggtgct
     2581 tctcaccggc tgtagcgcgt tcaatcaact ggttgagcac atgcaaaccg ctacgctgga
     2641 ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc aggaagtcag
     2701 ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcgt ctggtgcacg
     2761 ttacactgac ggaatctatg ttttctggtc gaaaggcgat gaagcgactg tctataaacg
     2821 cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag atttttccag
     2881 gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg tctgataacg
     2941 acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg ttacgccgtc
     3001 gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag gcgtgtgaag
     3061 ccaaactggc agaccctacc gcgatggtgg tcgctaccgt ggatgaacat gctcagcctt
     3121 atcaacgcat cgttttactc aaacattacg acgaaaaagg catggtgttt tacaccaacc
     3181 tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg ctgttcccgt
     3241 ggcataccct tgaacgccag gtgatggtga tcggtaaagc agaacgactt tcgactctcg
     3301 aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca tgggtttcga
     3361 agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg gagctgaagc
     3421 agaagtttca acagggcgaa gtgccattgc cgagcttttg gggcggtttt cgcgtcagcc
     3481 ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc tttttgtacc
     3541 agcgagaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
     3601 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
     3661 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt tgatggcaag
     3721 cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga cggacgagga
     3781 agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct tcgatcctac
     3841 cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac gcttccagca
     3901 ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg gcgacccgag
     3961 cttcaaagct gccgagcgta agctgaacac cgaagaaact gttcaggagt gggtggacaa
     4021 aatccgtaag caggttgccc cgttcctcga tttcgactgt ggagaaaact ctgctatcgc
     4081 ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc gcgatattgg
     4141 caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc gtctcaaccg
     4201 tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg gttatgactt
     4261 cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg accagtgggg
     4321 taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg tgtttggcct
     4381 gaccgttccg ctgatcacta aagcagatgg caccaaattt ggtaaaactg aaggcggcgc
     4441 agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct ggatcaacac
     4501 tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca ttgaagagat
     4561 caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc agtatgtact
     4621 ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg caaaacgtat
     4681 taccgaatgc ctgttcagcg gttctttgag tgcgctgagt gaagcggact tcgaacagct
     4741 ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcgct
     4801 ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg cctccaatgc
     4861 catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg
     4921 tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc tgatttgctg
     4981 gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag
     5041 ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta
     5101 acagtgcggc agagtttcct atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg
     5161 ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg ccgcccagcc
     5221 atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc tgtgatgccg
     5281 tgttaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt atcgtccgtc
     5341 aggtgaaagc cgcgaatccg caggcgaaat atttttgcga tccggtaatg ggtcatccgg
     5401 aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac ggtttgcctg
     5461 ccagcgatat cattgcgccg aatctggttg agctggaaat actctgtgag catgcggtaa
     5521 ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg ccacaaattg
     5581 tgttggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa atgctgctgg
     5641 tcaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt atgcgccagc
     5701 cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt cagggggcaa
     5761 cgctgcagga ggcgctggaa catgtgaccg ctgcagtcta cgaaatcatg gtgaccacca
     5821 aagcaatgca ggaatatgag ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag
     5881 aacattactt cagcgcaaca aagctctgaa atatctcatt tcggcccaca acgacctgtg
     5941 ggccgatgga tattacttta agccttccgc tgacagcgcg tcttgtactt ccggacgttc
     6001 agccatacgt tgcataaatg ctgcaatgtg ctctaaccct tccagattca gtttcaccgc
     6061 gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc tttgcccgca
     6121 gatccagtgc tcatccttca gtgcctcgtt cacatattgc agcttcttct ccagctgcgc
     6181 gcgaactgtc ggtttgtact cttccggtgt atcagggcga aatagaggtg tgaaaccttt
     6241 atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg aaatactgtt
     6301 taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca taatcgctac
     6361 gccttccgtc agcaaagtac cgtcatccag cagcaatgca ggcacctgcc ccttagggtt
     6421 aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca cactgacgag
     6481 ggtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc aggcacccgg
     6541 tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt
     6601 tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga gtttccctga
     6661 attttacgct acagcagctt tcgccgcttt gtctgcagcg tcatcctgcg tcatacggtg
     6721 cagtttaggc gcggtcagca gcatcagtac tgcaataatg gcagtagcga caccaatctg
     6781 caagaatacg cgaccataaa cttccagtga catcagcggg tcggtaacgt tatccggcac
     6841 agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg tcaggaacca
     6901 gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca ttgccagacc
     6961 cagaccagag atcatcagtt ccccgatgct ctgcaggcca tagcttgcga ccagccagct
     7021 tacagacacg ataccagcgt cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc
     7081 accagaacac atcaccatgc cgattgcaaa cttggttggc atcggcaggg tatcgcccat
     7141 cttgttatag atagcggcca gaatcggact accgatgatg atccagaacg ggttcagtgc
     7201 ctgatactgc tcaggttcta cggccagacc cagaatggag tgctcaacgt tacgaatcgc
     7261 aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa tggcttcgag
     7321 catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg cgaaggcttc
     7381 tttaccgaag ataaccacga taccgaaggc aacaacgccc agcgccatac gcgcaacttc
     7441 ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa taatggtcag
     7501 cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact gtttaaccca
     7561 gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc tcaacgcaaa
     7621 cgcaacactc cagccgtatt tcgcggccag ccacggcgta gcaatcatag agaagaaaga
     7681 gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg ggtcgttttt
     7741 ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt taccgaccgc
     7801 aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg caaccagagc
     7861 ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac ccagtacctt
     7921 gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa aggaagagaa
     7981 aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa cagccataat
     8041 tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga tgagatagaa
     8101 cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttg ggtttgcagt
     8161 ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct cgcgttatgt
     8221 ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa tgttcactat
     8281 cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag gcggggaatt
     8341 caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa cggcagattt
     8401 tttcactgca ctgagagggg gatgatggct taacggcgat atgttctgct atttagcgtt
     8461 tttactagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt gtaggtcaat
     8521 atttgttgcg tgtgtaaatt taaatgattt taacctgatt cttgcaatgt ttttacattg
     8581 cgacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa aaaaacaa
//
LOCUS       DAZFAH010000178         8523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFAH010000178 DAZFAH010000000
VERSION     DAZFAH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.180"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2590
                     /gene="cas3"
                     /locus_tag="ACHJWB_003885"
     CDS             <1..2590
                     /gene="cas3"
                     /locus_tag="ACHJWB_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO4315436.1"
                     /translation="LCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAW
                     LLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLP
                     SSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPL
                     IEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLI
                     QAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTG
                     AGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVL
                     AHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQ
                     AMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSA
                     TLSQQQRDKLVAAFAWGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSV
                     SVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHS
                     RFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLL
                     IQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVY
                     PDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDR
                     AMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWE
                     MSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEG
                     LIGKVG"
     gene            2688..4250
                     /gene="casA"
                     /locus_tag="ACHJWB_003886"
                     /gene_synonym="cse1"
     CDS             2688..4250
                     /gene="casA"
                     /locus_tag="ACHJWB_003886"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4315437.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4247..4783
                     /gene="casB"
                     /locus_tag="ACHJWB_003887"
                     /gene_synonym="cse2"
     CDS             4247..4783
                     /gene="casB"
                     /locus_tag="ACHJWB_003887"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4315438.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4795..5850
                     /gene="cas7e"
                     /locus_tag="ACHJWB_003888"
     CDS             4795..5850
                     /gene="cas7e"
                     /locus_tag="ACHJWB_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4315439.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5861..6607
                     /gene="cas5e"
                     /locus_tag="ACHJWB_003889"
     CDS             5861..6607
                     /gene="cas5e"
                     /locus_tag="ACHJWB_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4315440.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6589..7239
                     /gene="cas6e"
                     /locus_tag="ACHJWB_003890"
     CDS             6589..7239
                     /gene="cas6e"
                     /locus_tag="ACHJWB_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4315441.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7236..8156
                     /gene="cas1e"
                     /locus_tag="ACHJWB_003891"
     CDS             7236..8156
                     /gene="cas1e"
                     /locus_tag="ACHJWB_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4315442.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLSCRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8156..8449
                     /gene="cas2e"
                     /locus_tag="ACHJWB_003892"
     CDS             8156..8449
                     /gene="cas2e"
                     /locus_tag="ACHJWB_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4315443.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT         2102 a         2001 c         2363 g         2057 t
ORIGIN      
        1 tctttgctgg cattcgctgg atgtagccgc gatgggctat ttaatggtta aaagaaattg
       61 cttcgggctg gctgattatt ttcgtcaatt agggatttct gacaaggaac aggcggctca
      121 atttttcgcc tggttgctgt gctggcacga tattggaaaa tttgcccgct cttttcaaca
      181 actttacctg ccccctgaac tcaagattca ggaaggtgcc agaaaaaatt acgaaaaaat
      241 ctctcattca acgctgggct actggttgtg gaatcattat ttaagtgaat gtcaggagtt
      301 acttccttca tcttcgctct ctccgcgcaa acttaggcga gtgatagaaa tgtggatgcc
      361 cgtgacgaca gggcatcatg gtcggccccc tgatcgtatg gatgaattgg ataattttct
      421 gcctgaagac aaagctgcag cacgagattt tctccttgaa atcaaaccac tgtttccgct
      481 catagagatc cccgcattct gggatgatga tgagggcatt gaacttataa aacacctttc
      541 ctggtatatc tctgcaaccg tcgtgctcgc agactggacg ggttcgtcaa cgcgattttt
      601 tccacgcgtc gcacacccaa tggatattaa aggttactgg cagaaaactt taattcaggc
      661 tcaaaacgcc ttaaccgtct ttcctctaaa agcaaaagtc gcgcctttca acggaattaa
      721 tacgctgttt ccttttattg agaatccgac accgttacag caaaaggtgc tggatctgga
      781 tatcagccag caaggaccgc agttatttat tctggaagat gtgactggcg caggtaaaac
      841 agaagcggcg cttatcctgg cgcacaggtt gatagctgcg gggaaagctc aggggctgtt
      901 ttttggtctg ccaacaatgg caacggccaa tgccatgtac gatcggctgg tcaaaacctg
      961 gcttgctttc tattcgccag aatcccgccc cagcctggtg ctggcacata gtgcccgcac
     1021 attaatggac cgctttaatg aatcactctg gtctggtgat ttagtcgggt cagaagaacc
     1081 ggatgaacaa acgttcagtc agggatgtgc ggcctggttt gccgacagta acaagaaggc
     1141 gttgctggct gaaattggcg tcggcacgct ggatcaggcg atgatggcgg tgatgccgtt
     1201 caaacataat aatctgcggc ttctggggct gagtaacaaa atcctgctgg ctgatgagat
     1261 ccacgcctgt gacgcttaca tgtcgtgcat tcttgaaggg ttgatcgagc ggcaggcgcg
     1321 tggcggaaac agcgttattt tgctttctgc cacgttatct caacagcagc gcgacaaact
     1381 cgtcgctgcc tttgcgtggg gtacagaggg ccagcaagaa gctccgttcc tggaaaagga
     1441 tgattacccc tggctgacgc atgtcacgaa atccgatgtg aactcacacc gggtagcgac
     1501 gcgcaaagac gttgagcgta gcgtcagcgt gggttggctt catagtgaac aagagtgtat
     1561 tgcgcgtatc gaatcggcgg taagtcaggg gaaatgcatc gcctggatcc ggaattctgt
     1621 cgatgacgct attaaggttc atcgtcagct gcttgcccgc ggcgtcattc ctgcttccag
     1681 cctttcactt tttcatagcc gctttgcttt tagcgatcgc cagcgaattg aaacggaaac
     1741 gctggcacgc tttggtaaat attgtagttt acagagagcg tcgcaagtca ttgtttgtac
     1801 acaagtaatt gagcaaagcg tggatattga cctggacgaa atgatctccg acctggcccc
     1861 tgttgatttg ctgattcagc gagcaggacg attacagcgg catatccgcg atattaatgg
     1921 tcagttaaag cgtgacggaa aggacgagcg ttccccccct gaattgctga ttctggctcc
     1981 cgtttgggac gactctcctg gcgacgaatg gtttggcagt gccatgcgta acagtgcatt
     2041 tgtctatccc gatcatgggc gaatctggct gacgcagcgt gtgctgcgtg agcaaggtgc
     2101 tattcagatg ccacacgcag cccggcttct tattgaatcc gtctacggtg aggacgtggt
     2161 aatgccggaa ggatttgccc gcagcgagca ggagcaagtg ggcaaatatt actgtgatcg
     2221 cgcaatggct aaaaagtttg tgctgaactt caggcccggc tatgccgcca atatcaacga
     2281 ttacctcccg gaaaaactgt cgacacgtct ggctgaggag tctgtttccc tgtggctggc
     2341 tacctgtatt gacggtgtgg tgaagcctta tgccaccggt gctcacgcat gggaaatgag
     2401 cgttgtcaga gtgcgccgaa gctggtggaa aaaacatcgg gatgagtttt ctttactgga
     2461 gggagaggcg ttcaggctgt ggtgcattga acagcgacaa gatccggaga tggcaaacgt
     2521 gattttagtc aacgatgacg aaagttgcgg gtattcggct acggaaggat tgattggcaa
     2581 ggttggttga gatgtctgcg acaggactct ctgaatagtt gatacaccga aaaggatgga
     2641 aagatggaca accgacctgg gatggtcttt tttacgagag gatgcgtatg aactcgtttt
     2701 cacttctgac aaccccgtgg ttgcccgttc gttttaaaga cggaacgaca ggcaaactgg
     2761 cgccagtcga tctggcggat gaaaatgttg tcgatatttc tgcgccgcgg gcagatctcc
     2821 agggtgcggt atggcagttt ttgctggggc tactacaaac cagcttcgcg ccaaaagatc
     2881 atcgtcgttg ggatgatatc tgggaagacg gactggaagc tgaaaagcta cgggaagcat
     2941 tgcagtcatt agaacatgct ttccagtttg ggccagattc cccttcattt atgcaggatt
     3001 tcgaggcgct caccggagat aaagttccgg tcgcttcgct actgcctgag attcccggcg
     3061 ctcaaacaac gaagtttaat aaagaccact ttatcaagcg tggtgtaacg gaatacctgt
     3121 gcccacattg ttctgcgtta gctctgttct ccctacagtt gaatgcgccg tcaggtggca
     3181 aaggctaccg caccggttta cgcggcggtg ggccgatgac cactctgatt gaattacagg
     3241 aatatcaggg taatcaacaa actcccttgt ggcgcaaact gtggatcaat gtcatgccgc
     3301 aggatgaagc cgatttaccg ctacccaaaa aatttgacga tctggttttc ccctggcttg
     3361 gcccgacgcg caccagcgaa ctggccggtg cggtggtaac cgatgagcag gtcaataaac
     3421 tccaggcgta ctggggaatg cctcgccgta ttcgtattga ttttaatacc acgacagtcg
     3481 gcaactgcga tatttgcggt gagcagagcg acgcgctcct gagcctgatg acgaccaaaa
     3541 attacggtgc gaattatgcc atgtggcagc atcccttaac gccttaccgt gtaccactta
     3601 aagagggtgg cgagttttac tccgttaaac cacaaccggg cggtttaatc tggcgtgact
     3661 ggttaggcct tatcgaaacg ggtaaatcag aaaacaatac ggaacttccc gcgctggtgg
     3721 tgaaactctt caatgccagc agtctcaagc aggcaaaagt gggcctgtgg ggatttggct
     3781 atgatttcga caacatgaaa gcgcgctgtt ggtacgaaca ccatttcccg ctgctgctcg
     3841 ataaaaaaga aggtcagata ccgaagctgc ggctggctgc gcaaacagct tcacggattc
     3901 taagtctgtt acggagtgca ttgaaagaag catggttctc cgatccaaaa ggtgcaaggg
     3961 gtgattttag ttttgtggat atcgacttct ggaacaaaac tcagcatcgc tttctgaggt
     4021 tagtgcgcca aattgaagaa ggtcaggatg cggatgaatt actcagcaaa tggaataagg
     4081 aaatttggtt attcgcacgt caggattttg acgagcgtgt attcaccaat ccttatgagc
     4141 ccgttgatct ggaacgcgtc atgaccgcgc gcaagaaata ttttacaaca tcggcggaga
     4201 agcaaagtgc taaagccgcc agggagaaaa agcaggaggc tgctgaatga gtattgtaaa
     4261 agaggagcac aaagccacgc tgcgcaaatg gcacgaggaa ttgcaggaga aacgtggcga
     4321 acgtgccagt ctgcggcgta gtacgacggt taatgatgtt tgtctgacgg atggatttcg
     4381 cctgtttctg aagaacagac aaataaagtg gcaggatgaa cctgagtggc gtatcaccgc
     4441 actggcgctg atagccgcag ttagcgccaa tgttaaagct attgatgaac ggcagccttt
     4501 tgccgcgcag ctggcggcgg taatgtcaaa ggggcgtttt actcgtctgt cggcagtaaa
     4561 aacaccggat gaattactgc gccagttacg ccgcgcagtc agattattaa acggttccgt
     4621 caatcttgac tctctggcag agggggtctt ccgttggtgc caggagagcg acgacctgct
     4681 caatcatcac cgccgccagc agcggccaac cgaatttatc cgtatccgct gggcgctgga
     4741 atattaccag gctggcgacg ccgataacga acaaaatcaa taaaggacta tctcatgaca
     4801 acatttattc agcttcattt gttaaccgct taccctgctg ccaaccttaa ccgtgatgat
     4861 accggtgcgc caaaaactgt ggtgctgggt ggagcaacgc gtctgcgcgt ttcctcgcaa
     4921 agtctgaaac gtgcgtggcg cacttctgca ctttttgaac aggcactggc gggccatatt
     4981 ggtattcgca gtgggcgtat tgcgcgtgag gcggcaacta tcctgattga gaaaggaatc
     5041 gaagagaaaa aagccatcga atgggcggca aaaattgcag attatcttgg gaaagctaaa
     5101 aacgacaaaa aaccaaaaga tccgctcact aacgccgaaa ctgaacagtt agtccatatc
     5161 agcccggcag aatttgacgc cgtaaaagcg ctggcccatc agcttgcaga agaaaagcgc
     5221 gcgccaaaag aggaagatct cgcgttgtta cgcaaagacc gtatggcggt agatattgcc
     5281 atgtttggtc gtatgctggc gaataaaccc gagtttaatg ttgaagccgc ctgccaggta
     5341 gcgcacgcat ttggtgtcag tgaaacgatt gtcgaagatg attttttcac cgccgttgat
     5401 gatttgcgcc aggcttctga agatgccggt gccgggcatc ttggtgaaac cggattcggt
     5461 tctgcactgt tctacaccta tatctgcatt gataaagatc tgctggtcga aaaccttggc
     5521 ggtgacgaag cgttagctaa tcagaccata cgtgccttta cggaagccgc acttaaagtc
     5581 tccccaaccg gcaaacagaa cagctttgcc agccgtgcct acgcctcctg ggcgctggca
     5641 gaaaaaggca ccgatcaacc acgctctctg gcggcggctt tctatgaacc cattaatggc
     5701 acacgtcagt tagatgtggc ggtgcagcgt attacaacgc ttcgcgaaaa catgaatacg
     5761 gtctacgaac agaagaccga atgcgcaagt tttgacgtga tgaacaaaca gggaagcatg
     5821 aaggacgtgc tggactttat ctgcgcgtaa ggggcggctt atgagccaat atctgatttt
     5881 tcagcttcat gggccgatgg cgtcctgggg ggtcgatgct ccaggtgaag ttcgtcatac
     5941 ccatgaactt ccttcgcgct cagcattgct ggggctactg gctgccgggg tagggattcg
     6001 gcgtgatgat accgaacgct taaacgcgtt taatcgccac tattcactgg tggtttgcgc
     6061 cagccgtaac ccgcgctggg cacgggatta tcacacggtc cagatgccaa aagaggtgcg
     6121 taaagcacgt tatttcagcc gtcgcgaaga gttgagcgac cctgagcttt tgagcgcgat
     6181 tatctcccgg cgcgactact acaccgatgc ctggtggatg gtggcggtgg caacaacagc
     6241 cgatgcgcct tacagcctcg aacagttgca ggacggttta cgccatccag tttttccgct
     6301 ttatctgggg cgaaaaagtc atccgctggc attacctctt gcgccgttac tgctcgaagg
     6361 caacgcgtgt gatgccttat gtaatgcata ccaacagtat caggatcatt tccataaact
     6421 gaaagtctca ctcccgaaac ttcaggatga atgctggtgg gaagggaaac acgacggcct
     6481 ggtcgcgagc aaaatattac gccgccggga tgttccttta aatcgtcagc agtggctgtt
     6541 tggggaacgc accgtcaatc aggggccgtg gctcagcaag gaggaaccat gtacctctca
     6601 agaataacct tacataccgg ccagctttcg cctgcgcagt tgctgcatct ggtggatcgc
     6661 ggtgaatatg tgatgcatca gtggctgtgg gatctcttcc ctggcggcaa agaaaggcaa
     6721 tttctttatc gtcgggaaga actccagggc gcgtttcgct tttttgtact gtcgcaggaa
     6781 cgcccggcgg aaagtgatac gttcaccata gaatgccggt catttgcgcc cgagctgtgt
     6841 accggacaac aactttgttt caacctgcgg gcgaatccaa caatctgtaa agcgggcaag
     6901 cgccacgatc tgctgatgga ggcgaaacgg caggtgaggg ggcaggcgga aggaagtgat
     6961 gtctggttgc atcaacaaca ggcggcgctg gactggctgg cagcgcaggg ggagagaagc
     7021 ggttttacac tgctggatac ctcggttgat gcctaccgac aacagcaact gcggcgggaa
     7081 aactcccggc aactgatcca gttcagtagc gtggattata cggggatgct tacggtcacc
     7141 gatcctgggt tatttgtgca acgcctgagc caggggtatg gtaaaagccg ggcgttcggc
     7201 tgtggtctga tgctgatcaa acccggagca gaggcgtgac gtttgtacca ctgagcccga
     7261 tcccattaaa agatcgcacc tccatgatct tcctccagta cggtcagatc gacgtactgg
     7321 acggcgcttt cgtgctgatc gacaaaacag ggatccgaac gcatattccg gtgggatcgg
     7381 tcgcctgcat tatgctcgaa ccaggaacga gagtttccca cgcggcggtg catctggccg
     7441 ccacggtggg aacactgctg gtctgggtcg gtgaagcggg cgttcgcgtt tactcctccg
     7501 gacaacccgg tggggcgcgg gcagataaat tactctacca ggcaaagctg gctttaacgg
     7561 aagatctacg gttgaaggtg gtgcgcaaaa tgtatgaatt acgttttcgt gaaccaccgc
     7621 cagctcgccg ttcagtggag cagttacgtg gaattgaggg atctcgcgtt cgccagacgt
     7681 atgcattact ggcgaaacaa tatggtgtga aatggaatgg tcgcaaatac gatcctaaag
     7741 actgggaaaa aggcgatgtt gtgaatcgct gcatcagtgc tgctacatca tgtctgtacg
     7801 gtatatctga agcggcagta ttagccgcgg gatatgcgcc cgctattggg tttatccata
     7861 gtggcaaacc gctttcattt gtttatgaca tagccgatat cattaaattt gattcggttg
     7921 tgccaaaggc atttgaaata gcagcgaggc aacccgcaga acctgataaa gaagtcagat
     7981 tatcctgtcg cgatattttc cgtagcacta agttaacggg caaattaata ccgttaattg
     8041 aggaagtcct tgccgcaggt gaaattgaac cgccacaacc tgcgccggat atgttaccgc
     8101 cagccatccc cgaacctgaa acgttgggcg atagcggtca tcgaggacgc ggctgatgag
     8161 catggtcgtg gttgttacag aaaatgtccc gccgcgctta cgtggacggc tcgcaatctg
     8221 gctactggaa gtgcgtgccg gtgtgtatgt tggtgataca tcaaaacgta ttcgggagat
     8281 gatctggcag caaattaccc aactggctgg ttgcggaaat gtggtgatgg cctgggcgac
     8341 caataccgag tcgggttttg aatttcagac ctggggagaa aacagacgta ttccggtgga
     8401 tttggatggg ttacgtttgg tttcttttct tcctgttgat aatcaataag ttatccgttc
     8461 tttaaaaata aggaaatgtt ttaatttagt tggtagattg ttgatgcgga ataaatttgt
     8521 tta
//
LOCUS       DAZFAH010000179         8507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFAH010000179 DAZFAH010000000
VERSION     DAZFAH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.181"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            295..1272
                     /gene="glaH"
                     /locus_tag="ACHJWB_003893"
     CDS             295..1272
                     /gene="glaH"
                     /locus_tag="ACHJWB_003893"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4315444.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDFDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1292..2560
                     /gene="lhgO"
                     /locus_tag="ACHJWB_003894"
     CDS             1292..2560
                     /gene="lhgO"
                     /locus_tag="ACHJWB_003894"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4315445.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2583..4031
                     /gene="gabD"
                     /locus_tag="ACHJWB_003895"
     CDS             2583..4031
                     /gene="gabD"
                     /locus_tag="ACHJWB_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4315446.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4045..5325
                     /gene="gabT"
                     /locus_tag="ACHJWB_003896"
     CDS             4045..5325
                     /gene="gabT"
                     /locus_tag="ACHJWB_003896"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4315447.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFIQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLVIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5636..7036
                     /gene="gabP"
                     /locus_tag="ACHJWB_003897"
     CDS             5636..7036
                     /gene="gabP"
                     /locus_tag="ACHJWB_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4315448.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7057..7719
                     /gene="csiR"
                     /locus_tag="ACHJWB_003898"
     CDS             7057..7719
                     /gene="csiR"
                     /locus_tag="ACHJWB_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4315449.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7720..8169)
                     /gene="kbp"
                     /locus_tag="ACHJWB_003899"
     CDS             complement(7720..8169)
                     /gene="kbp"
                     /locus_tag="ACHJWB_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4315450.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8253..8411)
                     /locus_tag="ACHJWB_003900"
     CDS             complement(8253..8411)
                     /locus_tag="ACHJWB_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4315451.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1920 a         2224 c         2371 g         1992 t
ORIGIN      
        1 tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
       61 taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc
      121 tcactttgcc ttaataccct acgttaaatg ttactaattt gttgcttttg atcacaataa
      181 gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg
      241 ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat
      301 gcactgaccg ccgtacacaa taacgctgtc gattcaggcc aggactatag cggattcact
      361 ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc
      421 aaacagtttc tcgaacaggt tgccgaatgg cccgtgcagg cgctggagta caaatcgttt
      481 ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg
      541 ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc
      601 gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt
      661 ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat
      721 gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac
      781 ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac
      841 atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat
      901 ttccgccatc cactggcgcg tcgcccaatg cgctttgccg cgccgccgag caaaaacgtc
      961 agcaaagatg ttttccatcc agtgttcgac tttgatcaac agggtcgccc ggtgatgcgc
     1021 tatatcgacc agttcgtcca gccgaaagac ttcgaagaag gcgtgtggtt gagcgagctt
     1081 tcggacgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc
     1141 ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     1201 ctgcgccgtg aactgatgcg tcagcgcggc tacttcgctt acgccactca ccactaccag
     1261 acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg
     1321 catcatcggc atgtcgaccg ccatgcaact gattgatgtc tatccggatg cccgcattgc
     1381 gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat
     1441 ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcgggaaa
     1501 ccgcgccacc aaagcctttt gcgatcaaaa cgacattcgc tatgacaact gcggcaagat
     1561 gctggtcgcc acgtccgatc tcgaaatgga acggatgcgc gcgttgtggg aacgcacggc
     1621 ggcgaacggt atcgagcgcg agtggttaaa cgccgaggaa ctgcgcgagc gcgaaccgaa
     1681 tatcaccggg ctcggcggca tttttgtgcc gtccagcggc attgtcagct atcgcgaagt
     1741 cacagcggca atggcaaaaa tcttccaggc cagaggcggt gagattattt ataacgccga
     1801 agtcagcgcc ctcagtgagc ataaaaacgg cgtggtcgtc cgaacccgtc agggcggcga
     1861 atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat
     1921 gctcggcctc gaaccgggtt ttattatctg cccgttccgt ggcgagtatt tccgccttgc
     1981 gccggagcat aaccagattg ttaaccatct gatttacccc attcccgacc cggcaatgcc
     2041 atttttgggc gtgcatctca cccgaatgat cgatggcagc gtcaccgtcg ggccaaacgc
     2101 ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gcgacacgct
     2161 ggagattttg ggctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact
     2221 gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cgcctggtgc aaaagtattg
     2281 tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt
     2341 atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca
     2401 cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt
     2461 cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc
     2521 acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa
     2581 caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc
     2641 tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc ggcgaacggc gacaagctgg
     2701 gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc tatcgacgcc gccaaccgcg
     2761 ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg cgcaactggt
     2821 tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgacccta gaacagggca
     2881 aaccactggc cgaagcgaaa ggtgaaatca gctacgccgc ctcttttatt gagtggtttg
     2941 ccgaagaagg caaacgcatt tatggcgaca ccattcccgg tcatcaggcc gataaacgcc
     3001 tgattgttat caagcagccg attggcgtta ccgcagctat cacgccgtgg aacttcccgg
     3061 cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga
     3121 agcccgccag tcagacaccg ttctctgcgc tggcgctggc ggagctggcg attcgcgcgg
     3181 gcattccggc tggcgtgttt aacgtggtca ccggttcggc aggcgcggtc ggtaatgaac
     3241 tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttcgaccgaa attggccgcc
     3301 agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg
     3361 cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct
     3421 cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg
     3481 gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg cacatcggcg
     3541 acgggctgga taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag
     3601 tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag
     3661 cggatgaacg cggcggcaac ttcttccagc cgaccattct ggtggacgtg ccggccaacg
     3721 ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgc tttaaagatg
     3781 aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc tatttctacg
     3841 cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc atcgtcggca
     3901 tcaataccgg cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa gcctcgggtc
     3961 tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca
     4021 tcggtcttta actggagaat gcgaatgaac agcaataaag agttaatgca gcgccgcagt
     4081 caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac
     4141 tgccgggtgt gggacgttga aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg
     4201 ctcaataccg ggcacctgca tccgaaggtg gtggccgcgg tggaagcgca gttgaaaaaa
     4261 ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt
     4321 atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc
     4381 gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc
     4441 gcttttagcg gcgcgtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg
     4501 aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct
     4561 tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccatcg gatcttcaaa
     4621 aatgatgccg cgccggaaga tatcgccgcc atcgtgattg agccggttca gggcgaaggc
     4681 ggtttctacg ccgcgtcgcc agcctttatc cagcgtttac gcgcgctgtg tgacgagcac
     4741 gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt
     4801 gcgatggagc agatgggcgt tgcgccggat ctcaccacct ttgcgaaatc gatcgcgggc
     4861 ggcttcccgc tggcgggcgt caccgggcgc gcggaagtga tggatgccgt cgctccaggc
     4921 ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggcagcgct ggaagtgttg
     4981 aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg atctggggca gaagttgaaa
     5041 gacggattgc tggtgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga
     5101 gcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc
     5161 accgccgaga tcgtggcacg cgcccgcgat aaaggcctga ttcttctctc ctgcggcccg
     5221 tattacaacg tgctgcgcat ccttgtaccg ctcaccattg aagacgctca gatccgtcag
     5281 ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagctatg tccgtttgta
     5341 agagttaaac gttgctaagg gaatgataac gatgcagtaa aaaataataa cgatgaggcg
     5401 atcgacaaag ttaaaaacga ctgcggactg tcccctcgcc cctctgggga gagggttagg
     5461 gtgaggggta atcacgcggc tacaccctca ccccggccct ctccctaagg gagagggaga
     5521 aaaccctccc gctttcgttt ttaagatttt gttgaccgtc tcaattaaac gcgtgacccg
     5581 acgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg
     5641 gcaatcatcg caaccacatg agttaggcgg cgggctgaag tcacgccacg tcaccatgtt
     5701 gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga
     5761 agcgggcccg gcggtattac tggcctatct gttcgccgga ttactggtgg ttatgattat
     5821 gcggatgttg gcggaaatgg cagttgccac gcccgatacc ggttcgtttt ccacctatgc
     5881 cgataaagcc attggccgct gggcgggtta taccatcggc tggctgtact ggtggttttg
     5941 ggtactggtt atcccgctgg aagccaacat cgccgctatg atcctgcact cgtgggttcc
     6001 aggcattccc atctggttat tttccctcgt cattaccctc gccttaactg gcagtaattt
     6061 attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc
     6121 tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc cttatgccga
     6181 agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc
     6241 ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat
     6301 tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacta actcggttat
     6361 ctggcgtatt tctatcttct atttgtgctc tatttttgtc gtcgtggcat taattccgtg
     6421 gaatatgccc ggactaaaag ccgtcggttc ttatcgctcg gtactggaat tgctcaatat
     6481 tccccatgcg aaattaatca tggactgtgt gatattactt tccgtaacca gctgtctgaa
     6541 ctcggcgctg tataccgcgt caaggatgct ctactcctta agccgtcgcg gtgatgcgcc
     6601 cgcggtaatg ggcaaaatca accgcagtaa aaccccgtat gtggcggtgt tactctccac
     6661 cggagcggca tttttaacgg tggtggtgaa ctattacgca cctgcgaaag tgtttaaatt
     6721 cctgatagac agctccggtg ctatcgccct gctggtttat ttagtcatcg ccgtttcaca
     6781 gttgcggatg cgtaaaattc tgcgagcaga aggaagcgaa attcgcttgc gcatgtggct
     6841 ttacccgtgg ctcacctggc tggtaatagg ctttattacc tttgtgttgg tggtgatgtt
     6901 attccgcccg gcgcaacagt tagaagtgat ctctaccggc ttattagcga tagggattat
     6961 ctgtaccgtg ccgattatgg cgcgctggaa aaagctggta ttgtggcaaa aaacacccgt
     7021 tcataatacg cgctgataat gatcaggagt cacaccatga ccattacgtc tctggatggc
     7081 tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga aaaattacga
     7141 atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttacggga agctctttcg
     7201 caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct
     7261 atgtcagagc aggagctgct cgatattttc gacgcccgcg ccaatatgga agcgatgctg
     7321 gtcagtctgg cgattgcccg cggtggcgat gaatgggagg cagacgttct cgcaaaagcg
     7381 catctgctga gtaagctgga ggcctgtgac gccagcgaga aaatgcttga tgagtgggat
     7441 ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg
     7501 caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc gatttatctg gctgcggcga
     7561 acggtgcttt cggtagaaat gctggaggat aaacacgatc agcaccagac cctgactgcg
     7621 gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg
     7681 ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa
     7741 cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat
     7801 tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc
     7861 agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc
     7921 aacactggca ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg
     7981 actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc
     8041 ggcatcaggt ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt
     8101 atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca caaaattgaa
     8161 cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc
     8221 ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg
     8281 tgaatcaaac cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa
     8341 ccgaaccctt taccgagcag cacgccgaga ggcggcagaa tgatggtgat gacgattctc
     8401 cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc
     8461 tcttaaacgt agtaatttta gcggagcctg ccagtttcag cttacgc
//
LOCUS       DAZFAH010000180         8491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFAH010000180 DAZFAH010000000
VERSION     DAZFAH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.182"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            131..319
                     /locus_tag="ACHJWB_003901"
     CDS             131..319
                     /locus_tag="ACHJWB_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4315452.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            377..931
                     /locus_tag="ACHJWB_003902"
                     /pseudo
     CDS             377..931
                     /locus_tag="ACHJWB_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            1124..1321
                     /locus_tag="ACHJWB_003903"
     CDS             1124..1321
                     /locus_tag="ACHJWB_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4315453.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            1314..1526
                     /locus_tag="ACHJWB_003904"
     CDS             1314..1526
                     /locus_tag="ACHJWB_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315454.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            1516..1980
                     /locus_tag="ACHJWB_003905"
     CDS             1516..1980
                     /locus_tag="ACHJWB_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315455.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1973..2206
                     /locus_tag="ACHJWB_003906"
     CDS             1973..2206
                     /locus_tag="ACHJWB_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315456.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            2212..2511
                     /locus_tag="ACHJWB_003907"
     CDS             2212..2511
                     /locus_tag="ACHJWB_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4315457.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            2508..3908
                     /locus_tag="ACHJWB_003908"
     CDS             2508..3908
                     /locus_tag="ACHJWB_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4315458.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTKNHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            4109..4360
                     /locus_tag="ACHJWB_003909"
     CDS             4109..4360
                     /locus_tag="ACHJWB_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315459.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            4357..4767
                     /locus_tag="ACHJWB_003910"
     CDS             4357..4767
                     /locus_tag="ACHJWB_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4315460.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            4778..5050
                     /locus_tag="ACHJWB_003911"
     CDS             4778..5050
                     /locus_tag="ACHJWB_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4315461.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            5007..5135
                     /locus_tag="ACHJWB_003912"
     CDS             5007..5135
                     /locus_tag="ACHJWB_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4315462.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            5177..5401
                     /locus_tag="ACHJWB_003913"
     CDS             5177..5401
                     /locus_tag="ACHJWB_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315463.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            5653..5859
                     /locus_tag="ACHJWB_003914"
     CDS             5653..5859
                     /locus_tag="ACHJWB_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315464.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            5859..6914
                     /locus_tag="ACHJWB_003915"
     CDS             5859..6914
                     /locus_tag="ACHJWB_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4315465.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            6927..7262
                     /locus_tag="ACHJWB_003916"
     CDS             6927..7262
                     /locus_tag="ACHJWB_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4315466.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            7275..7688
                     /locus_tag="ACHJWB_003917"
     CDS             7275..7688
                     /locus_tag="ACHJWB_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO4315467.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            7888..8430
                     /locus_tag="ACHJWB_003918"
     CDS             7888..8430
                     /locus_tag="ACHJWB_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4315468.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
BASE COUNT         2385 a         1922 c         2286 g         1898 t
ORIGIN      
        1 agattttatt cttacttttt cattttctgt aaatcataca tcctcagtat ttgccattca
       61 tcactctagc gggaacaata cgacacaata agacacctga tgactcttta agaaacgaaa
      121 gggggcaata gtgttaagca ctgatcggtt tatacgtgaa aaagaatgcg aaaaactaac
      181 cggccttagc cgtacgtgtc gctaccgcct ggaaaaggcc ggacaattcc catcacgtcg
      241 taaacttggc ggtcgttccg ttggctggtc tttatccgag gttctggcct ggaaggatag
      301 ctgcaaggca gttcattaat cacgctggcg gcacacagcc gccacacatc aatcatctga
      361 acacagagct ataaccatga agattgaata tacgccagaa cgtgggcggg gattcgttcg
      421 ccctggtgag actggaaaac cacaaaattg gggtttttca ggcataaaaa aagcggcccc
      481 gaaatggagc cgcctttctg aacagataac ccgctgcgcc ttatgtgtat atgatcccaa
      541 acataagcac ggggatgata gccgctatca ggctggtggg caatgctatc agtctggttc
      601 agttcgttgc catacctgca atgggcgcgt ttccctgtac tctttaagga attgctcaag
      661 ggcaaaagca catggcgcga atctttctga ttcatgctct atctttctgc gccgtctttt
      721 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
      781 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
      841 gcgttgctgt aattctttgc ggagaacgcg tttaatccaa gtggctaaag atgtgtcgcc
      901 atcggcctta gctgcttctt ctagctgcgc tctgaagtct tccggtaatc gcatttggta
      961 tggaggcgaa cgtttttcta atggtgttga catggtaatt accctttatt actataccta
     1021 catggtaatt accattgtac ttgccattac acaaaaatca acgccccgca gtgctgggaa
     1081 cacatacggg gcgtctaacc aacaacgtaa actaggagcc gttatggttg ccgtaaatca
     1141 tataccacac cttgtacaca cacaaacggc ctttgtgtgg cgttttctgg cactgagtgc
     1201 cggagaatct caaatcatcc acgtaaccgc ctggacggaa cgcgaagcgc gtaaccgttg
     1261 cccgtccggt tgtgttgctg tatttgccgc ccgtattcgt caggaggtgc gccatgatta
     1321 atctgtcact tactgacctt aaccgcattc agtttcgtga gaaattcaca gggcagctac
     1381 tggtcaatgt ggataaaggt cgcgtggtgt gtaattacca cttaccagat gaagcaattg
     1441 tcgccacaag ggaatcatta caggaaatca caaaacgtgc cggaatgatt aacacgaata
     1501 ttcaggggat gctttatgca caataaaacc acaccggacg cagccgccga agccatcaaa
     1561 acgctgatgc acgcgctgat tgatatttct gttatcgcgg acagggcaca taagcacgcc
     1621 accagcgaaa cagaatatgc cggtgctttc gttcctcact cactggctgt catgcaattt
     1681 agtgctgata cggcgctgaa tgaggccaaa gctatcctga ttgctgattg tgaaaatggg
     1741 ggggttatgc gtgatgatcg ttttaattcc ctgaaacagg aattttccgg cgttcctgat
     1801 gatgcggctg atgcgctttc gtcaatgcca gaacttatta gagcggcttt tttcttactt
     1861 tccacgagag aatataaatc aacggggctt gatgtactga atatcgccgc cgattatgcg
     1921 gaatatgtgg cagaggcgcg ttatagaaga aaatttcctg aggatgtaag ccatgcgtga
     1981 tatttaccac caaacaatag accgcgcatt tcttgcactt tctcacagtg aaaacatgat
     2041 ggaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc
     2101 aagaattgcc acggcattaa taacgcttct tgagcctgta ataatggaac tgcaagaaat
     2161 agatctattg cacgacagat ataaagaaca gcacaccgga gaataaaaat aatgaaactt
     2221 aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg cggtgatatt
     2281 taccgcgacc agtacggcgg cacggtaatg attaagggcg tggaagaacg gcgcgtaacc
     2341 taccgccgtg aaggttacga atatgattgc gtgatgcctg tttatcagtt ccggcgtgat
     2401 ttttctctgg tacagaccgc gccgcataac gtgcccacca gcaacgccag ggcacgggca
     2461 aacatccaga agctgaaaac catggttaac ggattcaggg gcaagaaatg aaaagcgcac
     2521 cgaacttaaa aaaacagcct tacgacaaga tgaccgaagt cattattttt gcgggtagtg
     2581 atgcctgggc acatgcgaaa cagtggcagg aacaggacgg gcgactggct ggcgataatg
     2641 tgcctcccgt tgtgctggct gatgatcaac tggatgaact ggcagacctg agaatcatcg
     2701 acgaggggcg ctattgtgtc cggctgtaca aggcaggcca catcaggcca tcaaatatta
     2761 atgccattgc gcacaagctg gcggcggcgg gtgtaactga tgcgaattat taccccgaag
     2821 ggatgcacag ccatatgcgg gagaactggc gcgaatacct ggaacgggtg cgcgggaaag
     2881 agccggcgga agaaaaaaac caccagcgaa aaaccacgct accgatgagc gttggatcta
     2941 ccggatacga cacgcaactg gattacgtgg ttaaggggat tattccggcg gtatcgctat
     3001 gcagcatata cggggctagc gggtcctata aatcattcct tgccggatcg tgggcgtgcc
     3061 atgttgccac tggtcgccag tggggaggcc gcagggttgc acatggtgcg gttctctatg
     3121 tggttggtga aggcggtata ggtgttccgc gtcgtgtaaa agcctgggag gttgtgcacg
     3181 atgagcaggt gaaaaatctg tatctggtaa accgccccat ctttccggct gccccgcttg
     3241 atgttgatga aatggttatc gctgcccgtc aggtggagcg ggaaacgggt aaacctgtac
     3301 gcatgattat tctggatacg ctggcgcgtt gctttggtgg gaatgatgaa aatgattccc
     3361 gtgatatggg ggcgtttatc cgtggttgtg acgaactgaa acgacgcaca ggggccacgg
     3421 tgctggtggt tcaccattcc ggcaaggatg agacgaaagg cgcgcgcggt tccagtgcat
     3481 ttcgtgcttc gctggatgct gaataccgga tacgcaggga ggacgcagga agcgaagcgc
     3541 tggttatctc atgcaccaaa atgaaggacg cggaggaact caaagaagcc gcatatgact
     3601 tacgcgtggt ggagcttttt accgacgctg acggtgaatt aatcacgtcg ctggtggtgg
     3661 tggatgatcc gcgccctcct gttgaactgg agcgcatcga ggaggcaggg aacaagacga
     3721 aaaaccatac cgcgctatgg gggtgcatcc gttcacgcac acagaacggc gacaagtgca
     3781 cgatcccgct gttacgtgat gacatgaaaa agctggggta tgaaatgaaa aacttccggc
     3841 gctggctgta caagctggaa aaagatgggg ttattcgtat cgatggggat gatgtagcgc
     3901 cgctataaaa gtgaggagca aaagcgaggg ggatagaaag agggccaaaa ttagcccgct
     3961 ctccctcact tttcgacctg tatacatcct caaaagtgag gggtaaaaaa atacttatga
     4021 aacacacaca tagaaaaacc gaaaatccca actgcgacga agtgagacgc ttgaaaaagt
     4081 gaggcgaaaa agtgagaggt tgcgagaaat gacccaaaaa cgcagagaca gaacagagcc
     4141 aaaatataaa gcgttagaca tgactgagca caccttaaag gtggcaatca gaacgataga
     4201 ccgccacacg cgggaaggat acgcgaagga acatcccgac ctgataagcg cattcatgac
     4261 cacggcggcg gcaaactttg ccacgctgac agaacgggag attgccgaag cggaacaggt
     4321 aacaaccatc aacgttaaaa ccggagaggt ggaattatga cggcacagat agccgcttac
     4381 gggcggctgg tggacgaccc gcaggtaaaa cagaccagca agggcacacc gatgacgctg
     4441 gcgcgtatgg cggtatcttt gccatgcagc caggcacagg acggacaggc gacgttatgg
     4501 ttatcggtca tcgcatttgg caaacaggcc gacttcctgg ctaaacatca aaaaggcgat
     4561 gttgccagcg tatccggcac gatgcaggta agccagtgga ccggacagaa cggagaaacg
     4621 cggcagggct ggcaggttat cgcagacagc gtaatcagtg cccgcgcggc acgtccaggc
     4681 gggaacagac gcaaaaccac aggcacacag ggtaatcagc caccagcggg aggcgatgac
     4741 ccttacggtg atgatatttc gttctgaggg ggtgacgatg gtacatgacc gcatagcgga
     4801 ggaactggag gcgaaaggct tttaccggag ggcggcggcg cgatggggtg aagtcatgca
     4861 gctggtggag acagacaagg aacggcatca catcacgatg cgacggctgg aatgttcaag
     4921 gaaggcacag agggcaccgg agccgccgga taatttcgga gacctgaaaa aggcagtcga
     4981 tcgcacttat gccgaaatgg gtatagatgg tgctggtgat gaaatatggc gcaattacca
     5041 ggacagctaa tcaaacagcc ggagaaatcc ggcttttttt gcaccagttg aaacggtatg
     5101 gcgcattacc gggttttcgt cacggtcagg catagttact atctgaaaca aacagacaca
     5161 acagaggaaa aaaacaatgc cgatgaaatt tgatgagata ttaaaacagc gtgataaata
     5221 ccatgctgac aacatggaga cgatgagcat caatgattac cgcgcattcc tggagacggg
     5281 cgcactgatt gaaaaggatc agcatggttt tgtgagatgt gctctatccg gtgaaatgct
     5341 ggcggtaaat cctgaacaga tagatgcatt gatagaattt ctgaaagaga tcagagactg
     5401 agccagcaca cagcacacat agccggagca atccggcttt tttgcgccca aaaaaagccc
     5461 gataaggtca gagggttctt atcgggcttt tgcatatgag gtttttggat gcactggcgt
     5521 tcgtgatcgg gataatcatt tcataatttg caacataact caatatcatt gcataaaatg
     5581 caattctgat tataatcagg actggataaa catccagtta tgattttttt agtcgaagag
     5641 gaatttctta ctatggctga agagaaaaaa ggcggtgttt cggtgtacat aagccccgaa
     5701 atcgtggagg tgctcaagca gcgccacaaa aaaaactatg aagctggcgt tgcggctgga
     5761 ctggatccgc tgatgacgcc ggagccgtcg ataggttcac ttgtacgctc ttatttactt
     5821 gcggcgcttg ggatgcataa aaattatggg ggtgaataat ggcaggcaaa gcaacggcac
     5881 ttaacactaa ccagcttttc atgtacctga atcgcgggga tattgcggat tttaaattca
     5941 gccctctgtt taccacgctg tttttcccga acgtggcgac attcagcacg caaaacatca
     6001 tgctggatac cctggacatt gaagaagtca ccatgtcggc gttttgttcg cctatggtgg
     6061 gtagccaggt tcagcgcgat aaagggtacg aaaccagcac aatcaaacct ggctacatga
     6121 agccaaagca cgaaatcgat ccaacgaaaa ccatcatgcg catggctgga gaagatccgg
     6181 cacagcttaa cgaccctacc tatcgccgta tgcgcctgat tactggcaac atgcgccgcc
     6241 agataaacgc cattaaggca cgcgtggaat ggctggcggt gaatgcggta acgaccggaa
     6301 aaaacatcat tgagggcgaa ggcatagaac gctatgaaat cgactggaaa ataccggaaa
     6361 actgcatcat agagcaggcc aagggtaaaa aatggtccga gcaggataaa gacatgcacg
     6421 acccaatcta tgacatcgag ctttatgctg atcaggctgg ttgccccgca aacgtcatga
     6481 ttatgggcgc tgaggtatgg cgcacattac gcagctttaa aaaattccgt gagctgtacg
     6541 atctttcccg tggttcagaa tccgccgccg aactggcctg taaaaacctg ggcgaagtgg
     6601 tgagctttaa aggctatctt ggtgatctgg cccttatcgt ctattccggc aaatacactg
     6661 acagcgatgg taccgaaaaa tatttccttg agcctgattt gctggtcctg ggtaacacca
     6721 acaataaagg gctggtggcc tatggtgcga taatggatca ggaagcggta agaacgggcg
     6781 caacacaaaa catgttttac ccgaaaaact ggattgagga cggcgatccg gcgattgagt
     6841 acgtgcagac acacagtgca ccgcagccgg taccggcaga tattcgcaaa tttgttaccg
     6901 tcaaaattgg ttaacggggg gattctatgg acactccata cattgagtta tttgcaggca
     6961 gtcagcaggt atccacgacg ttggtacatt ttgccgctga tgctggcgtt attcaggaat
     7021 ttaccccgct gatgctggca gacaatggcg agtttaagcc gtgggatggt caggaatctg
     7081 gcaaggctgt ttatctgacc tcgtaccctg tggacacgtc aaagcagaaa tcagcacagt
     7141 gttacaagac ggggatattc aatattgccg ccgttaactg gcctgagagc gtcgacaccg
     7201 atgcgaaaaa atgcgccgcc tttgcgggtt ccggcgtatc cgttcagccg ctggcccgat
     7261 aagcaggggg aacgatggca acgaatgaaa gcatcatgac gctaccgctg gcgagtaaat
     7321 ttaaagtcga agcgcgggca atggctgaca gaggtttatc aacctatgag gccgtatatc
     7381 aactaaacaa actggaagag caggacaagc cgcgcgctga tgcgattatg gcgcttcatg
     7441 agcataaaga ctatcagccg ctgttgcgtg caatggcaaa cgtgccttgt atcgatgttg
     7501 gtacggctaa aagcatcctt agcatgacca tagagcagga acgtccgaag gttgcgccgg
     7561 aacttaccgc agcctttgaa aactttatgg acatgcacag cccgcaagcc gtatcagctg
     7621 gcatggcata cgatggcaga aaccagggcg atgacggcga catcgatcgc atactgaaaa
     7681 ccatctgaga caaggccgga gaaatccggc ttttttacgg gtccttcctg gaattatggc
     7741 ccgttacggg gcggcgacct cgcggatttt cgctatttat gagccttttc agggtggtgg
     7801 ttttgttgtt tgatctatct ttatgaatga aaagggaaag atgcaagcaa tacaccaacc
     7861 tgaagcagta attaagttgg tgtattaatg aaatctcatc tgatgaacaa aaaaaacatg
     7921 gcgaaaagct gccgtgtaag tgcgacagcg ttcgacaagt ggggagtaac tcccgttgag
     7981 cgtaaaggcc gtgaggcgtt ttatgatgtt gccagcgtga tagaaaatag ggttaacaat
     8041 gcaattagcc agcttataaa cgacaaaggc gagattgacg atgatgaact tttacgagtc
     8101 aggatcagat tactgacagc acaggcagag gcgcaggaac ttaaaaacga tcgcgatcgc
     8161 ggtgacgtga ttgatactga gttttgcctg tacgcgcttt caaagctggc gagtcagatt
     8221 tcatcaatca tggacagcct cccgcttact atgcaaagga gcttcccaca gattaccccc
     8281 gccatgctgg acaggcttaa aagggaagtg gttaaagcct gtaatgccag tgccagagtt
     8341 gccgacaacc tcccacagat actggctgat tacttgaaag aaacaaccgg aaacgtaccg
     8401 gaaaagttgc tacagaagaa aggcgagtaa cagacgcgca attattgaac aaaaatgaga
     8461 aaggatctga taatggcatt ttctggctaa a
//
LOCUS       DAZFAH010000181         8485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFAH010000181 DAZFAH010000000
VERSION     DAZFAH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.183"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..436)
                     /locus_tag="ACHJWB_003919"
     CDS             complement(<1..436)
                     /locus_tag="ACHJWB_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4315469.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVG"
     gene            694..1299
                     /locus_tag="ACHJWB_003920"
     CDS             694..1299
                     /locus_tag="ACHJWB_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4315470.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1339..2202
                     /locus_tag="ACHJWB_003921"
     CDS             1339..2202
                     /locus_tag="ACHJWB_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4315471.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEIIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2192..3739
                     /gene="fdrA"
                     /locus_tag="ACHJWB_003922"
     CDS             2192..3739
                     /gene="fdrA"
                     /locus_tag="ACHJWB_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4315472.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3739..5157
                     /locus_tag="ACHJWB_003923"
     CDS             3739..5157
                     /locus_tag="ACHJWB_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4315473.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5300..6250
                     /locus_tag="ACHJWB_003924"
     CDS             5300..6250
                     /locus_tag="ACHJWB_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4315474.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAHEIHADILVITTGVEKVCIHFGKPEQQALDRVDNATMTRYMQEGHFPPGSML
                     PKIIASLAFLEQGGKEVIITTPECLPAALRGETGTHLIKT"
     gene            6260..7642
                     /locus_tag="ACHJWB_003925"
     CDS             6260..7642
                     /locus_tag="ACHJWB_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4315475.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            7941..8351
                     /locus_tag="ACHJWB_003926"
     CDS             7941..8351
                     /locus_tag="ACHJWB_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4315476.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
BASE COUNT         2162 a         2136 c         2321 g         1866 t
ORIGIN      
        1 tccccacgca gttacccgcc agaaaaccca gtaacgtcac cgtccagttc atcccgctgg
       61 caaaaaacag cgtcatgccg agaaagccac cgggaataaa agacagtaac cagaaacgcc
      121 cttgccagac cactactgca gagaaaggca cggtagtaat cacgctcgcc cagaacccag
      181 aaatcccact caccgaggcc agccaaccgg cagccagcgc ggcgaaaaac gcccacacca
      241 tcccggaata attaaccgcc agacttctga taaaccccga tttaccgccg cctgcggcat
      301 aaaaactgca aaaggcgacg aagccaatgg ttcctaataa ttcccatccc tgagagagcc
      361 cgacatgtga agacactaac tgccaaagcc ctgcacaaat accgacggtg acacccgttg
      421 ccgtgagtcc gttcatcttg ttcccctctg gttagacatg gacaaaagct actcttacat
      481 gtgcagacta tagagaaata aaacctgatt gagtgaaagg gtatcgggtc aaagaaacaa
      541 atattgcact accgcacact gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa
      601 tacggcgaat taaatttaag cgggttcttt tatcagatta tcgttgctat tctcaattca
      661 tcgaaaacag ccacaaaaac caggggaatg tgaatgagta taaaaaatct acctgccgac
      721 tatttattgg ctgcacaaca gggtgatatc gataaagtaa aaacctgcct tgcgctgggt
      781 gtcgatatta atacctgcga tcgtcagggg aaaacggcaa ttacgctggc aagtttatat
      841 cagcaatatg cttgcgttca ggcattaatt gacgccggag cggatattaa taaacaagat
      901 catacctgtt taaatccttt tttaattagc tgtctgaacg atgatttaac actactacga
      961 attattttac cggctaaacc cgatcttaat tgcgtaaccc gttttggcgg tgtcggcctg
     1021 acgcctgcct gtgaaaaagg ccatttaagt attgtaaaag agcttttggc gcatacggag
     1081 attaacgtta accagaccaa ccatgtaggc tggacgccgc tactggaagc gattgtgctt
     1141 aatgatggtg gtattaaaca gcaggcgatt gtgcagttat tgctggaaca cggtgccagc
     1201 ccgcatctga ccgataaata tggcaaaacg ccactggaac tggcgcggga acggggcttt
     1261 gaagagattg cgcagttact gattgccgca ggtgcataaa ccgggaggct tgctatcaac
     1321 acaccagaaa aacggtgtgt gtgggcgcta actgcggatg cggattttct ggcgcagcgg
     1381 gggcaaggac aggttgaaca ggtctttgcc agagcggtaa atatcgcact cccggctcgc
     1441 cagcagttgc tgacgctgct ttgtgaagag tacgacaatg cgccaaacag ttgtcggttg
     1501 gcactcactc actttgatga tctgttccgg catggtgata aggttcagtt tgacgatcaa
     1561 ggtattacgg ttggtcaaca tcttcatata gagatgagtc gttgtcggcg ttggctgtcc
     1621 ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg cctggcaaca gtggcacgac
     1681 attattcatc agcacctggg ggaaaatgaa accctgttta attatcgcgg cgataatccg
     1741 ttttatcagg cgttaaataa agaattacat attaaacgac gggcagttat tcagacagta
     1801 aacgataagc aaaatatcgc cgcagcggtc gccagtatga tggggttagg gattggcctt
     1861 acgccatcag ccgatgatta tttaacaggt ctggcgctta ttttatttat tcccgggcat
     1921 ccggcagaaa aatacaaaga ggaattttat cgcggtctgc tacgcggcag aaataacacc
     1981 acgttattaa gtgccataac gctggaagcc gcattacaac aacgctgccg ggaaaatatt
     2041 catcgcttta ttcacaacat tatttatggc gtccctggga attcaactca ggcaatagaa
     2101 ataattaaac atattggctc cagttccggc tgcgacatgc tgtatggcat ggccgatggt
     2161 tgtgcgctga gccaaaccta cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa
     2221 tacctatttt gattctgtct cgctgatgtc tatctccacg cgtgcaaata aactcgacgg
     2281 cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac aaaggtgtgc tgaaaaattt
     2341 aggactgctg acgccggagc tggaacaggc gaaaaacggc gacctgatga ttgtcatcaa
     2401 tggtaaatcg ggtgcggaca atgagcagtt gctggcggag attgaagaac tgttcaatag
     2461 caaagcgcaa agcggctcgc acgaggcgcg ttacgccact attgccagcg ccaaaaagca
     2521 tatcccggaa agtaacctgg cggtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc
     2581 gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt tccgataacg tctcagttga
     2641 agatgaactg gcgctcaagc agttggccca cgaaaaaggg ctgctgatga tggggccaga
     2701 ctgtggcacg gcgattatca acggcgcggc gctctgtttt ggtaacgccg tgcgtcgcgg
     2761 caacatcggt attgttggcg catccggcac cggcagccag gagctgagcg tccgcattca
     2821 tgaatttggt ggcggcgttt cacaactgat tggcaccggc gggcgcgacc tgagcgagaa
     2881 aatcggcggc ctgatgatgc tcgacgccat cgggatgctg gaaaacgatc cgcaaactga
     2941 aattatcgcg ctgatttcaa aacctcccgc acctgcggtg gcccgcaaag tgctggaacg
     3001 cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc ggtcgcgttg aaacgccggt
     3061 ggatgagcag gggctacagt ttgcccgcgg caccaaagag gcggcgctaa aagcggtgat
     3121 gctctccggc gtgaaacagg aaaatctcga cctgcatacg cttaaccagc cgttgattgc
     3181 ggatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt cgtggcctgt tctgcggcgg
     3241 cacgttgtgc gacgaaacca tgttcgcggt gatggaaaaa catggcgatg tctacagcaa
     3301 cattcagccc gatccggaat tccgcctgaa agatatcaac cgcagcatca aacacacctt
     3361 cctcgacttt ggcgatgacg acttcaccaa cggcaagccg catccaatga ttgaccccac
     3421 caaccgcatc agtcgcttga tcgaagaagc gcgcgatcca gaagtggcgg tgatcgtgat
     3481 ggattttgtg ctcggatttg gatcgcatga agatccggtc ggctccacca tcgaggcgat
     3541 caaagaagcg aaagcgatcg ccgccgccga aggacgcgag ttgatcattc tcgcctatgt
     3601 gctgggcacc gatctcgata cgccatcatt agaacaacaa agccagatgt tgcttgatgc
     3661 cggagtgatt ctggcgagca gcagcaccaa taccggattg ctggcgcgtg aatttatctg
     3721 caaaggggag gaagcctgat gagccagtca ctgtttagcc aaccattgaa cgttattaac
     3781 gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc atgtagaagt gacccaactc
     3841 gactggacgc cgccggggca gggcaatatg caggtggtgc aggcgctgga taacattgcc
     3901 gattcgccgc tggcggacaa aatcgccgcc gctaaccagc aggcgctgga gcgcattatt
     3961 cagtcgcatc cggtgctgat tggttttgat caggcgatta acgtggtgcc gggcatgacg
     4021 ccgaaaacca ttcttcacgc cgggccgccg atcacctggg aaaaaatgtg cggcgcgatg
     4081 aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg cgaaagatct cgacgaggcg
     4141 gctgacctgg cggcctccgg ggagatcacc ttctcgccgt gccacgaaca cgactgtgtg
     4201 ggatcgatgg cgggcgtcac ctcggcctcg atgtttatgc acatcgtgaa aaacaaaacc
     4261 tacggcaaca tcgcttacac caatatgagc gagcagatgg cgaagatttt gcgtatgggt
     4321 gccaacgatc agagtgtgat cgaccgcctg aactggatgc gcgatgtgca ggggccaatg
     4381 ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc gcttaatgct ggcgcaggcg
     4441 ctgcatatgg gcgatgagtg ccataaccgc aataacgccg ggacgacgtt gctgattcag
     4501 gcgctgacgc cggggattat tcaggcaggt tattccgtgg agaaacagcg cgaagtgttt
     4561 gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt ggatggcgat gtgtaaagcg
     4621 gcgatggatg cggcgcacgg catcgaatac agcaccgtgg tcaccaccat ggcgcgtaac
     4681 ggcgtcgagt tcggcctgcg ggtcagcgga ttaccggggc aatggtttac cggtccggcg
     4741 cagcaggtca tcggcccgat gtttgccggt tataagccgg aagattcggg gctggatatc
     4801 ggcgacagcg ccatcactga aacctacggt attggcggat ttgctatggc gaccgcgcct
     4861 gcaatcgttg cgctggtggg cggcacggtg gaagaggcta tcgatttctc tcgccagatg
     4921 cgtgaaatca ctctcggtga aaaccccaac gtcaccattc cgctgctcgg ttttatgggc
     4981 gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg gcattctgcc ggtgatcaac
     5041 actgccatcg cccataaaga tgcgggcgtc ggcatgattg gcgcgggcat tgtgcatcca
     5101 ccttttgcct gcttcgagaa agccattctt ggctggtgcg aacgttacgg cgtctgacct
     5161 acatgttcat gccggatgcg gcgtgaacgc cttatccggc ctacggatgg cccgagaatt
     5221 gtaggcctga taagcgtagc gcatcaggca gtctggcgtt ggtcataacc ccatcacact
     5281 ctgtagcgga cataacaaca tgaaagaact tgtggtcgtt gccattggtg gcaacagcat
     5341 tatcaaagat aacgccagcc agtcgattga gcatcaggcg gaggcggtga aagccgtcgc
     5401 cgatacggtg ctggaaatgc tggcttccga ttacgacatt gtgctgaccc acggcaacgg
     5461 gccgcaggtc gggctggatt tacgccgtgc ggagattgcc cacgagcgcg aagggctgcc
     5521 attaacgccg ctggcgaact gtgtagcgga tacgcaaggc ggcattggct atctgatcca
     5581 acaggcgctg aacaaccgac tggcgcgtca cggcgagaaa aaagccgtca ccgtggtgac
     5641 tcaggtggaa gtggataaaa acgatccggg atttgcccat cccaccaagc ccatcggcgc
     5701 attctttagc gacagccagc gtgacgaatt acaaaaggca aaccctgact ggtgttttgt
     5761 tgaagatgcc gggcggggct atcgccgcgt ggtcgcctcg ccggaaccga aacgtattgt
     5821 cgaagcaccc gccattaagg cgctgatcca acaaggcttt gtggtgattg gcgcgggcgg
     5881 tggcggaatt ccggtagtgc gtactgaagc gggggattac caaagcgtgg acgcggttat
     5941 cgacaaagat ctctctaccg cgctgctggc ccatgaaatt cacgccgaca ttctggtgat
     6001 caccaccggg gtggaaaaag tgtgtattca ttttggcaaa cccgagcagc aggcgctgga
     6061 tcgcgtagat aatgccacca tgacccgcta tatgcaggaa gggcatttcc cgccgggcag
     6121 catgttgcca aaaatcatcg ccagcctggc gttcctggaa cagggcggca aagaagtgat
     6181 tatcaccacg ccggaatgcc tgcctgcggc gctgcgtggt gaaacgggca cccaccttat
     6241 taaaacgtaa ggacgtaaga tgaaagaaaa caatagccgc cgtgaatttc tgagccagag
     6301 cggtaagatg gtcaccgctg ccgcgctgtt tggtacctct gtgccgctcg cccatgcggc
     6361 agtctctggc acaacccact gcgacgcgaa taacaccatg aaaatcactg acccgcatta
     6421 ctatctcgac aacgtgctac tggaaaccgg ttttgactac gaaaacggtg tggcggtaca
     6481 gacccgcacg gcgcgccaga ccgtggagat tcaaaacggc aaaattgttg ccctgcgcga
     6541 gaacaagcag catccggacg ccacgctgcc gcactatgat gctggcggta agctgatgct
     6601 gcccaccact cgcgatatgc atattcatct cgacaaaacc ttctacggcg ggccgtggcg
     6661 ctcgctcaat cgtccggcag gcaccaccat ccaggacatg atcaagcttg agcagaaaat
     6721 gctgccggaa ctgcaaccgt acacgcagga acgggcagaa aaactgatcg atctattgca
     6781 gtcgaaaggc accaccattg cccgcagcca ctgcaatatc gaaccggttt ccggcctgaa
     6841 aaatctgcag aatttgcagg cggtgctggc gcgacgtcag gcgggcttcg agtgtgaaat
     6901 cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg gaacccttaa tgcgcgaagc
     6961 gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg accagtgttg atggcgcgat
     7021 ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac tacgacaaag gtgtcgatat
     7081 tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc aattatatgg ttgaaacggt
     7141 agagaaaacg ccgcaactga aaggtaagtt gaccatcagc cacgcctttg cgctggctac
     7201 gctcaacgaa caacaggtcg atgcgctggc gaatcggatg gcggcgcagc aaatttctat
     7261 cgcctcgacg gtgccgattg gcacgctgca tatgccgctc aaacagttgc acgacaaagg
     7321 cgtaaaagtg atgactggca ctgacagcgt tatcgaccac tggtcgcctt atggtctggg
     7381 cgacatgctg gaaaaagcca atctctacgc gcagctctat attcgtccta acgaacagaa
     7441 cctctcccgt tcgctgtttc tagccactgg cgatgtattg ccgctgaatg aaaaaggcga
     7501 gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg ctggtggacg cctcctgttc
     7561 cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg ttccataaag ggcaactggt
     7621 gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct gcaacggtga attgtaagag
     7681 atttaagccc cagggagtgg ggcgggaatg gtttgctaat gaatgatcct gaactttata
     7741 atctcgcggc ataatttcca ggggattatt tgagagaaat acacaaaagt gagttttaac
     7801 aaaggtattt atcaggccat aaatgtgacc agcttcggat aaaaaagatt cagtatcgaa
     7861 aaaccaatga tactcttgct gatggttacc aacagtaatt aagatgtgat ttgtttttcc
     7921 gtaatcgcaa aaatattgcc atgaccataa aagaaaaatt ctcacaaaaa tatccccatg
     7981 cttccttttg tacttttggc gattcagcag cgttggccga tcacctcgca acgttgattg
     8041 cgactggtgt taaaacagct tcctgtggtt cgctggcggg atgtattgaa gacaacgcat
     8101 ttccgatgat tggtgagtat aagattgtag aaaatagccg tggtgagcct gtatgtgtta
     8161 tcagagtgat tggcctgcat ttactgcgct tttctgatgt caccgtggag cttgcccgta
     8221 aggaaggtga aggtgacctc agtcttgaat attggcgtaa tgaacaccgt cgttttttcc
     8281 aggcagaagg aagctattca ccagaaatgg acgttatatt tgaagaatat gcccttattg
     8341 acgttgtgta gataactatt tatctacgcg aaataaactc acacacataa aatatcgcta
     8401 attttgttta ttttgctatt ttttacgtga taaatgtcac acttattgtt gctgcccgtt
     8461 ttgtccatat ttataaccat atttt
//
LOCUS       DAZFAH010000182         8447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFAH010000182 DAZFAH010000000
VERSION     DAZFAH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.184"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHJWB_003927"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHJWB_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4315477.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHJWB_003928"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHJWB_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4315478.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHJWB_003929"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHJWB_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4315479.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLDNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDHRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHJWB_003930"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHJWB_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4315480.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHJWB_003931"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHJWB_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4315481.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHJWB_003932"
                     /pseudo
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHJWB_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHJWB_003933"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHJWB_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4315482.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHJWB_003934"
     CDS             complement(7218..7283)
                     /locus_tag="ACHJWB_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315483.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHJWB_003935"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHJWB_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4315484.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2084 a         2236 c         2019 g         2108 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gctaatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggtgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta tccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcattgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg tacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg attttccaac cagggtcaaa tcggcgaata
     5941 cgataccgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttc
//
LOCUS       DAZFAH010000183         8385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFAH010000183 DAZFAH010000000
VERSION     DAZFAH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.185"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            306..1646
                     /gene="dcuB"
                     /locus_tag="ACHJWB_003936"
     CDS             306..1646
                     /gene="dcuB"
                     /locus_tag="ACHJWB_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4315485.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1724..3370
                     /gene="fumB"
                     /locus_tag="ACHJWB_003937"
     CDS             1724..3370
                     /gene="fumB"
                     /locus_tag="ACHJWB_003937"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4315486.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLIEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3493..4122
                     /gene="yjdF"
                     /locus_tag="ACHJWB_003938"
     CDS             3493..4122
                     /gene="yjdF"
                     /locus_tag="ACHJWB_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4315487.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4261..5682)
                     /gene="melB"
                     /locus_tag="ACHJWB_003939"
     CDS             complement(4261..5682)
                     /gene="melB"
                     /locus_tag="ACHJWB_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4315488.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5785..7140)
                     /gene="melA"
                     /locus_tag="ACHJWB_003940"
     CDS             complement(5785..7140)
                     /gene="melA"
                     /locus_tag="ACHJWB_003940"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4315489.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAVASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7423..8331
                     /gene="melR"
                     /locus_tag="ACHJWB_003941"
     CDS             7423..8331
                     /gene="melR"
                     /locus_tag="ACHJWB_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4315490.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2152 a         2211 c         2063 g         1959 t
ORIGIN      
        1 tgccctgaga acaaaaaata gaccgataaa tatcaataag ataacagcaa acaaaacatt
       61 aacatctgcg cagtacaaac tataaaccca tcgccagaga gtttttctct ctgaaaaagc
      121 cgcttatcac agtgcataaa tttgccgctg ctttaatcag ccaatattca ctgtgaggca
      181 tttgctaaag ccggtaacga ccaaacggat atttagtcag gctctgaaaa cagttcatac
      241 aaaacagaac gtgactgtga tctattcagc aaaaatttaa ataggattat cgcgagggtt
      301 cacacatgtt atttactatc caacttatca taatactgat atgtctgttt tatggtgcca
      361 gaaagggtgg tatcgcgctg ggtttattag gcggtatcgg tctggtcatt ctggtcttcg
      421 tcttccacct tcagccaggt aaaccaccag ttgatgtcat gctggttatc attgcggtgg
      481 tggcggcatc ggcgaccttg caagcttcgg gcggtcttga tgtcatgctg caaattgccg
      541 agaagctgct gcgccgcaac ccgaaatatg tctcaattgt cgcgccgttt gtgacctgta
      601 cactgaccat tctttgcggt acgggtcatg tggtttacac cattctgccg atcatctacg
      661 acgtcgccat taagaacaac atccgtccgg aacgtccgat ggcggcaagt tctatcggtg
      721 cacagatggg gattatcgcc agtccggtgt cggttgcggt cgtgtctctg gttgcgatgc
      781 tgggtaatgt cacctttgat ggtcgccatc ttgagttcct cgatctgctg gcaatcacca
      841 ttccatcgac gttaatcggt atcctggcga tcggtatctt cagctggttc cgcggtaaag
      901 atctggataa agacgaagag ttccagaaat tcatctccgt accggaaaac cgtgagtatg
      961 tttacggtga taccgcgacg ctgctggata aaaaactgcc gaaaagcaac tggctggcaa
     1021 tgtggatttt cctcggggca atcgctgtag tcgcccttct tggtgctgat tcggacctgc
     1081 gtccatcctt cggcggcaaa ccgctgtcga tggtactggt tattcagatg tttatgctgc
     1141 tgaccggggc gctgattatt atcctgacca aaaccaatcc cgcgtctatc tcaaaaaacg
     1201 aagtcttccg ttccggtatg atcgccatcg tggcggtgta cggtatcgca tggatggcag
     1261 aaaccatgtt cggtgcgcat atgtctgaaa ttcagggcgt actgggtgaa atggtgaaag
     1321 agtatccgtg ggcgtatgcc attgttctgc tgctggtttc caagtttgta aactctcagg
     1381 ctgcggcgct ggcggcgatt gttccggtcg cgctggcgat cggcgttgat ccggcataca
     1441 tcgtggcttc agcaccggct tgctacggtt attacatcct gccgacttat ccgagcgatc
     1501 tggcagcgat tcagtttgac cgttccggca ccacccacat cggtcgcttc gtcatcaacc
     1561 acagttttat tctgccgggg ttgattggtg tgagcgtatc gtgcgtcttc ggctggatct
     1621 tcgccgcgat gtacgggttc ttataaatgc actttgcgtg ccgcccgtga ctacgcggca
     1681 cgccattttc gaataacaaa tacagagtta caggctggaa gctatgtcaa acaaaccctt
     1741 tatctaccag gcacctttcc cgatggggaa agacaatacc gaatactatc tactcacttc
     1801 cgattacgtt agcgttgccg acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc
     1861 cctgacccta ctggcgcagc aagcctttca cgacgcttct ttcatgctcc gtccggcaca
     1921 ccagaaacag gttgcggcta ttcttcacga tccagaagcc agcgaaaacg acaagtacgt
     1981 ggcgctgcaa ttcttaagaa actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca
     2041 ggataccggc accgcgatca tcgtcggtaa aaaaggccag cgcgtgtgga ccggtggcgg
     2101 tgatgaagaa gcgctgtcga aaggcgtcta taacacctat atcgaagata acctgcgcta
     2161 ctcgcaaaac gcggcgctgg acatgtacaa agaggtcaac accggcacta acctgcctgc
     2221 gcaaatcgac ctgtacgcgg tagatggcga tgagtacaaa ttcctttgcg ttgcgaaagg
     2281 cggcggctct gccaacaaaa cgtatctcta ccaggaaacc aaagccctgc tgactcccgg
     2341 caaactgaaa aacttcctca tcgagaaaat gcgtaccctc ggtactgcag cctgcccgcc
     2401 gtaccatatc gcgtttgtga ttggcggtac gtctgcggaa accaacctga aaaccgtcaa
     2461 gttagcaagc gctcactatt acgatgaact gccgacggaa gggaacgaac atggtcaggc
     2521 gttccgcgat gtccagctgg aacaggaact gctggaagag gcccagaaac tcggtcttgg
     2581 cgcgcagttt ggcggtaaat acttcgcgca cgacattcgc gttatccgtc tgccacgtca
     2641 cggcgcatcc tgcccggtcg gcatgggcgt ctcctgctcc gctgaccgta acattaaagc
     2701 gaaaatcaac cgcgaaggta tctggatcga aaaactggaa cacaacccag gccagtacat
     2761 tccggaagaa ctgcgccagg ccggtgaagg cgaagcggtg aaagttgacc ttaaccgccc
     2821 gatgaaagag atcctcgccc agctttcgca atatccggtt tccactcgtt tgtcgctcac
     2881 cggcaccatt atcgtgggcc gagatattgc acacgccaag ctgaaagagc tgattgacgc
     2941 cggtaaagaa cttccgcagt acatcaaaga tcacccgatc tactacgcgg gtccggcgaa
     3001 aacccctgcc ggttatccat caggttcact tggcccaacc accgcaggcc gtatggactc
     3061 ctacgtggat ctgctgcaat cccacggcgg cagcatgatc atgctggcga aaggtaaccg
     3121 cagtcagcag gttaccgacg cgtgtcataa acacggcggc ttctacctcg gtagcatcgg
     3181 cggtccggcg gcggtactgg cgcagcagag catcaagcat ctggaatgcg tcgcttatcc
     3241 ggagctgggt atggaagcta tctggaaaat cgaagtagaa gatttcccgg cgtttatcct
     3301 ggtcgatgac aaaggtaacg acttcttcca gcagatcgtc aacaaacagt gcgcgaactg
     3361 cactaagtaa cctcttcggc ccagcgcctg gcagcatgct gccaggtgat ccccctgggc
     3421 cacctctttt gcgattgtaa tttcacgctt gctggtgaat agtcagtatt ttccctgact
     3481 tgcgaactca ccatgacccg cacactcaag ccgttaattc ttaacaccgg cgcactggcg
     3541 ctaacattaa tcctgattta taccggcatt tcggcccatg acaaactcac ctggctgatg
     3601 gaagtgacac cggtgattat tgtcgtgccg ctactgcttg ccaccgccag acgttatccg
     3661 ttaacgccgc tgctctatac gctcattttc tttcacgcca tcatcctgat ggtcggcggg
     3721 caatacacct acgcgaaagt ccccgttggt tttgaggtgc aggaatggct ggggttgagt
     3781 cgtaatccgt atgacaagct ggggcatttt ttccaggggc tggtgcctgc actggtggca
     3841 cgagaaattc tcgtgcgcgg gatgtacgtg cgcggacgta aaatggtggc gttcctggtc
     3901 tgctgcgtgg cgctggcgat aagcgcgatg tatgaattaa tcgagtggtg ggcggcgctg
     3961 gcaatgggcc agggagcgga tgattttctc ggcacccagg gcgaccagtg ggacacgcaa
     4021 tccgatatgt tctgcgcgct gcttggcgca ttaacgacgg tgatattcct cgctcgcttt
     4081 cattgccgcc agctacggcg ctttggcttg ataaccggat aacaacgtgc gctttgttga
     4141 tgccggatgc ggcgtgaatt ccttatccgg tctacaaaat ttgcaaatcc aaagcattgt
     4201 attaagacgt ggcctgataa acataacaca tcaggccatt ttcgttagct attgtccacg
     4261 tcaggctttc acatcactca ctgcaccgac cggaatatca gcatgaacag gctcgggcgg
     4321 tactttgcga tatttatcca gcagatggat ctggatcctg cgcagcgtgt caccattgag
     4381 gcgatagaaa cggaagtaga gaatcagcgt taccatgaaa aacagagttg gtagagcaat
     4441 cataataaac tgcataccta atagggcttg cgtagactgt tcaacattcg gtacatagcc
     4501 aatcatccct aacacaaccg caatgaaaaa agccgcaaag gctgaaccgc ccttcaccac
     4561 catagtctgc acggagtaag cgatactttc acagcgtacg tgcagtttat attcaccgta
     4621 atcaacggta tctgccacca tgatgacctg taatacccag aaaagcgccg ttcccacatt
     4681 cagcaaaata cccgcaatca caatgaggac gacgttgtga tagctcatta atgccattaa
     4741 caggagaaca ccacagctta acaccggaag aatagatgct ccggcccata aaatgcgtcg
     4801 ggataatgat ttaactaagc gtgggaagaa tactaacgtc accaggttag cagctcccgc
     4861 atacgacaga taatagggga acaaatccgc atcaccaata acatatgaga aataatagat
     4921 agcaaagccg gtaataatat tgctggctac attataagca agagccatac ccaagaggca
     4981 tgaaagctga tcgtttttat aaataagcgc aacgatggct ttaagtgtca gatggcttcc
     5041 ttcagcagac ggttgattgt ctgacgaaaa gacttcatgc acattgcgca gagtgatgat
     5101 ggttgaaaca ataaaaaagg cgatcagtac cagagtgaac atctgaaagc caaatccccg
     5161 atcgccaccg ccgacataat taacaaatgg tagcgtcaca cctgccgtaa caaagcccgc
     5221 cagactggca aaaaaacgcg gataaggaac cagttgttca cgctcacgtt tatcgagcgt
     5281 gatggttgga accagcgacc agaagggaat atccataatg gtgtaagtca tgccccagag
     5341 gatgtaggtc acgcaaacaa agacaatctg agtagtacct tcaaacagat gcgcactaaa
     5401 gaggagaaat aagattacag agtttgccaa cgtaccgatc aggatccagg gtttgaactt
     5461 accccatcgc gatcgcgtag catttacaat ccatcccata atcggatcgt taatagcatc
     5521 ccagatcctc gccaccagaa acaaagtacc gaccaaaccc acagacagcc caacgacatc
     5581 ggtgtagtaa tacatgaggt acatatacac aatgccgatc gcaaaatcct tcccgaacgc
     5641 tccaaatcca taactgagtt ttgtagtcat tgaaatgctc atagggtatc gggtcgctgt
     5701 taccaacgcc cgccttctgt tattggtatc gtcagaggtg tgcgccggaa taaatccccc
     5761 ggcgcagtag cgtttagtcg cgttttaacg gtgcaaccag cctggcagcc agtcgccgtg
     5821 ggcggcaatc aggtcgtcaa caagagcata tatttcgtca atgcccagca cggcggcagt
     5881 atgcgggtcc atcatcgcgg cgtggtaaac acggtcgcga ttttccgtaa gaatagcttc
     5941 ggtcagcagc gtctgtacgt tgatgttggt ttgcatcagg gcggccagat gcgaaggtag
     6001 cgtaccgact ttggtcggct gaatgccatt agcatcaacc agacaggcta cttccacgca
     6061 acatccttgt ggcaggttat caatcaaacc atcgttacgg acgttgccgt aaatcacact
     6121 cggctcgcca gtccagatag cgttcatgat tgtgctggca tattcccgtg acggtttaat
     6181 atcaatccgg gaggcgtttt tatactcctc cagctcttta tgccagttcg ccagctgctc
     6241 gacgcagcgt ttcgggtact catccagcgg tactttataa cgctcaatca aatcctcacg
     6301 acctggctta ataaaccacg gtgtgtactc agcaaaatgt tctgacgatt ccgtgacgaa
     6361 gtagcccagc tttttgaaca tttcgtagcg cacaatattc tggcagcgag tattaccatg
     6421 aatatttggc ttcggtgcct gccctgcttc ataagccgcc agcagttccg ggtagagatt
     6481 cacgtaactg ccgtcggcgg ttttacgctc cagttccagg taaaacgcca tatggttgat
     6541 accagcgcaa cggtaacgca gcgtggacgg atcgatattg aggtcacgcg ccagctcttc
     6601 cgccgttccc tgcaccgaat ggcacagccc tacctgtttg atatgcggat agcgggcata
     6661 catcgcccag gtattcatcg ccattgggtt aacgtagttg agcatggtgg catcggggca
     6721 gacttccgtc atgtcctcgc aaatttgcca cagatgcgga atggtacgta gcgcgcgcat
     6781 aataccgccc ggccccaacg tatcggcaat ggtttgttcc agaccatgcc gcttacagac
     6841 ctcgaaatca gtcaccgtgc aaggttcata accgccaatc tgaaatgcca ccacgacaaa
     6901 atcggcatcc tgtaaggctt ctttctgttg ggtgtggcag gtgattttgc cgctggccac
     6961 tgctgaatcc atcagcttac gcaccacaat atgcgactct tccaggcggg tgggatcaat
     7021 gtccatcagg gcaatatgcg ccgttttcag cgcctcgcga tggaacacat caccaagaat
     7081 atttttaacg aaaatcgtcg aaccagcgcc gataaatgta attttgggtg cagacatcat
     7141 gcagatctcc tggcttgctt gaataacttc atcatggcag gcgaatctgc agaaaacctc
     7201 cggcttccag gttgagcatt cccataaact cagatcctgc gtgaagcagc agtaaatctg
     7261 agtttatggt aataagttac ctgaaaagca gagttttcgg cctcatgatg ctcggacgct
     7321 cccctcgcct ctctgggaag agggtgaaaa ccgtgctccc actcgcagtc atcctccctc
     7381 actcctgcca taattctgat attccaggaa agagagccat ccatgaatac agatacgttt
     7441 atgtgcagca gcgacgaaaa acagacccgc agcccgctgt cgctgtattc ggaataccag
     7501 cggatggaga ttgagtttcg cgcaccgcat atcatgccca ccagccactg gcatggtcag
     7561 gtcgaagtga atgtgccttt cgatggcgat gtggaatacc tgatcaacaa tgaaaaagtg
     7621 aatatcaatc agggtcatat cacgctgttc tgggcctgta caccgcacca actaacagat
     7681 accggaacct gtcagagcat ggcgattttt aatctgccga tgcatctgtt tctctcctgg
     7741 ccgctggata aagacctgat taaccacgtc actcacggca tggtgatcaa atcactggcc
     7801 acacagcaac ttagcccgtt tgaagtgcgc cgctggcagc aggaattaaa cagtccgaac
     7861 gagcaaattc gccagctcgc cattgatgaa attggcctga tgctcaagcg atttagcctc
     7921 tctggctggg aaccgattct ggtcaataaa acctcgcgca cacacaaaaa cagcgtctcg
     7981 cgccatgcgc aattttatgt tagccagatg ctgggcttta ttgccgaaaa ctatgaccag
     8041 gcgctgacca tcaacgatgt ggctgagcac gtcaaactta acgccaacta tgcaatgggg
     8101 atattccagc gggtcatgca attgacgatg aaacagtaca ttaccgcgat gcgcatcaac
     8161 cacgttcgcg cgttactgag cgataccgat aaaagtattc tcgatattgc cctgacggca
     8221 ggctttcgtt caagtagtcg tttttacagc acgttcggca aatatgtcgg catgtcgccg
     8281 caacaatacc gcaaacttag ccaacagcgc cgccagacgt ttcccggcta aagacgatat
     8341 cagtatcagc caaaaaaata gtttagccgt cgataataag cagtg
//
LOCUS       DAZFAH010000184         8368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFAH010000184 DAZFAH010000000
VERSION     DAZFAH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.186"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(239..1129)
                     /gene="malG"
                     /locus_tag="ACHJWB_003942"
     CDS             complement(239..1129)
                     /gene="malG"
                     /locus_tag="ACHJWB_003942"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4315491.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1144..2688)
                     /gene="malF"
                     /locus_tag="ACHJWB_003943"
     CDS             complement(1144..2688)
                     /gene="malF"
                     /locus_tag="ACHJWB_003943"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4315492.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPTGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPLQNFFKITLPLLIKPLTPLMLASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2842..4032)
                     /gene="malE"
                     /locus_tag="ACHJWB_003944"
     CDS             complement(2842..4032)
                     /gene="malE"
                     /locus_tag="ACHJWB_003944"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4315493.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4397..5512
                     /gene="malK"
                     /locus_tag="ACHJWB_003945"
     CDS             4397..5512
                     /gene="malK"
                     /locus_tag="ACHJWB_003945"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4315494.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5584..6924
                     /gene="lamB"
                     /locus_tag="ACHJWB_003946"
     CDS             5584..6924
                     /gene="lamB"
                     /locus_tag="ACHJWB_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4315495.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADTNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7167..8087
                     /gene="malM"
                     /locus_tag="ACHJWB_003947"
     CDS             7167..8087
                     /gene="malM"
                     /locus_tag="ACHJWB_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4315496.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2070 a         2186 c         2162 g         1950 t
ORIGIN      
        1 tctaaaattg atgaaaacaa tactattgcc gtgactcaga gcacgaaaga gaattatcgt
       61 aagtgggaaa acaaataacg taaaaataat aagctctatg atggaaatat taaccggcga
      121 acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt ggagcgccga ataacagtca
      181 caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa caacatcttt
      241 aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga
      301 cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc cacaggtagt
      361 tttgcgggtt gaggtattgc tgcatcccca cggccagggt gtagctgttt acgtcacgca
      421 gtaacagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg atgaatacca
      481 ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg
      541 caccatccag cgcagccgct tcttccagcg aactatcgat ggtttcgaaa tagcctttga
      601 tggtccagac atgcagcgca atcccaccca gatacgcgaa aattacgccg ccgtgagtat
      661 tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac aacgcgacca
      721 gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc gtcgctttgc
      781 ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt agagagcgcc acaatgccaa
      841 tcgcggaaat cccggcgacc tttaccgagt tccatagcca cagcagtacc gggaatggcg
      901 gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc taacgccagt ttccagtgat
      961 cccaggagat ttgctccggg atcaggctgc cggtcgcaaa gttcccctgg cgcagtgaga
     1021 tagcgacgac catcagcagc gggaacataa tcgccgcgat aaaaagtagc agtagcaggt
     1081 gagtaataaa caaacgtgct ttttgcgatt tcggttggac cattgccatt tttgttatct
     1141 cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg cacccaccag
     1201 caggaagatc agcgtggcaa ttgctgccgc cagaccgaag tcctgacccc cgccgccttc
     1261 aaaagcgatg cggtaggtgt agttaacaag caggtcggta taaccggctg gcgtggtcgt
     1321 gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt tgttaaagtt
     1381 aaaggcgaag ctggcgagca tcagcggcgt cagcggttta atcagcagcg gcagcgtaat
     1441 cttaaagaag ttctgcaacg gacctgcacc atccattgct gaggcttcat acaaatcgtc
     1501 cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac ccagccaggt
     1561 attgacgata atcagcatcg tgcgggcggt ggtcggatcg ctgaaccagg cgggcttcac
     1621 gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag ctctggttaa acaatccttt
     1681 gaaaatcaag attgaaatga acgatggcac cgcgtagggc agaatcagca ggacgcgata
     1741 gaccgctttg ccgcgcaacg cttcccactg caccagacat gccagaacca tgccgaccgc
     1801 caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa
     1861 cggtttctga atgccttcgt cggtaaagac gcgggtaaag tttttccagc cggtggtcac
     1921 ggtgtaaccg gggcttagct tttcatcacc ccagttgccg tcggcggtaa tggactggta
     1981 aaagccaatt tggttattcg gacgatattt cacgccgctc tgattattcg tcaacgtgcc
     2041 gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg agctcatcat
     2101 cactttgttg ccatctggca gaatggcggt aatgtcactc agcgcctgac gattctgggt
     2161 aatcacgcgc agattcgcgc gttcaccttc gggctgggtg gtcgtttctt tcagttgcag
     2221 tttttgctcg ccgccaaatt taaacgcgtc ggaaaggtaa tttttgccgg tttcgccgtc
     2281 gctgagcgcc agttgccact catcgcccgt cgggtaaaga ccaaagttat aggttttgcc
     2341 tgcttgccag gagcgatcta acaacacttc ctgcgcacgt tcaaaagtca gctggttagt
     2401 gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga ggacgaataa
     2461 tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac gattggcgaa
     2521 aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt attccccttg
     2581 tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta gcactgacca
     2641 tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg ggttcttcct
     2701 cattccagga cggataaggc tttcacgcct tatccgacaa caactgcctg atgcgacact
     2761 gatgcgtctt atcaggccta catgcgtttc agttttgtag gccggacaag gcgttcacgc
     2821 cgcatccggc atttcatagc attacttggt gatacgagtc tgcgcgtctt tcagggcttc
     2881 atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
     2941 agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt ccatggtggc
     3001 ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcca ctgcacccag
     3061 cggtttgtct ttattgaccg cttccagacc ttcgtcagtc agcagatagt tttcgaggaa
     3121 ctctttcgcc agctctttgt tcggactggc ggcgttaata ccggcgctca gcacgccaac
     3181 gaacggttta gatggttgac ccttgaaggt cggcagtacc gttacaccat aattcacttt
     3241 gctggtgtcg atgttggacc atgcccacgg gccgttgatg gtcatcgctg tttcgccttt
     3301 attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat
     3361 aaggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt
     3421 aatatcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagcag caatcagcgg
     3481 ccaggtgaag tacggttctt gcaggttgaa catcagcgcg ctcttacctt tcgctttcag
     3541 ttctttatcc agcgccggga tctcttccca ggtttttggt gggttcggca gcagatcttt
     3601 gttataaatc agcgataacg cttcaacagc gatcgggtaa gcaatcagct tgccgttgta
     3661 acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt ccggggtgat
     3721 ttcagccaac aggccagatt gagcgtagcc accaaaacgg tcgtgcgccc agaagataat
     3781 gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt tatccggatg
     3841 ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt cagcgagacc
     3901 gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga ttttggcgag
     3961 agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt
     4021 ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga
     4081 ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc cccaccgttg
     4141 ctttgtgtga tctctgttac agaattggcg gtaatgtggg gatgcgcaca taaaatcgcc
     4201 acgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag ttcacagaag
     4261 ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg aggatttaag
     4321 ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg acaggttgtt
     4381 acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga
     4441 ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt
     4501 cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc ttgagacgat
     4561 caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc cagcagaacg
     4621 cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag tagcagaaaa
     4681 catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag gtgattaacc aacgcgttaa
     4741 ccaggtggcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga aagcgctctc
     4801 cggtggtcag cgtcagcgcg tggcgattgg ccgtacgctg gtggccgagc caagcgtatt
     4861 tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga
     4921 aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc acgatcaggt
     4981 cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gccggtcgcg tggcacaggt
     5041 tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg gatttatcgg
     5101 ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc accgcaatcg atcaagtgca
     5161 ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccggttgaaa gccgtgatgt
     5221 ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc cgagtgatat
     5281 cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca acgaaaccca
     5341 aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga acgacgtggt
     5401 gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt gccatctgtt
     5461 ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt aagcacccca
     5521 caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat
     5581 agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg
     5641 tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat tggctggaca
     5701 ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa ataccgtctt
     5761 ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg gaaagagggc
     5821 gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca gaatgactgg
     5881 gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg
     5941 ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc aacgtcatga cgttcatatg
     6001 atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa catcgatgtt
     6061 ggcttcggta aactctctct ggcagcaacc cgctcctctg aagctggtgg ttcttcctct
     6121 ttcgccagca ataatattta tgactatacc aacgaaaccg cgaacgacgt tttcgacgtg
     6181 cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt cgactacggt
     6241 cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga cggctggtta
     6301 ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt tgttcagtac
     6361 gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt
     6421 gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg tatcctcgac
     6481 cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat gtaccaggat
     6541 atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg cccgatgtac
     6601 aaatggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt cgaatcccag
     6661 cgcaccggcg acaagaacaa tcagtacaaa attaccctcg cacaacaatg gcaggctggc
     6721 gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag
     6781 aaatggggtt atgactacac cggtaacgct gataccaacg cgaacttcgg caaagccgtt
     6841 cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt
     6901 gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggtg cttgcaccgc
     6961 attcggcaac caacgcctga tgcgacgctt gcgcgtctta tcaggcctac aacggctgtc
     7021 aaatgtaggc cggataaggc gtttacgccg catccggcat aaaaacaggt tgtcattatc
     7081 tgaaaggggc gaaagcccct ctgattatcg ggtttagcgc gctattgcct ggctaccgct
     7141 gagctccaga ttttgaggtg aaaacaatga aaatgaataa aagtctcatc gccctctgtt
     7201 tatcagcagg gttactggca agcgcgcctg gaatcagcct tgccgatgtt aactacgtac
     7261 cgcaaaacac cagcgacgcg ccagccattc catctgccgc gctgcaacaa ctcacctgga
     7321 caccggtcga tcaatctaaa acccagacca cccaactggc gaccggcggc caacaactga
     7381 acgttcccgg catcagtggt ccggttgctg cgtacagcgt cccggcaaac attggcgaac
     7441 tgaccctgac gctgaccagc gaagtgaaca aacaaaccag cgtatttgcg ccgaacgtgc
     7501 tgattcttga tcagaacatg accccatcag ccttcttccc cagcagttat ttcacctacc
     7561 aggaaccagg cgtgatgagt gcagatcggc tggaaggcgt tatgcgcctg acaccggcgt
     7621 tggggcagca aaaactttat gttctggtct ttaccacgga aaaagatctc cagcagacga
     7681 cccaactgct cgacccggca aaagcctatg ccaaaggcgt tggtaactcg atcccggata
     7741 ttcccgatcc ggttgctcgt cataccagcg atggcttact gaaactgaaa gtcaaaacga
     7801 actccagctc cagcgtgttg gtaggaccac tatttggttc ttccgctcca gctccggtta
     7861 cggtaggcaa cacggcggca ccagctgtgg ctgcatccgc tccggcaccg gtgaagaaaa
     7921 gcgagccgat gctcaacgac acggaaagtt attttaatac cgcgatcaaa aacgctgtcg
     7981 cgaaaggtga tgttgataag gcgttaaaac tgcttgatga agctgaacgc ctgggatcga
     8041 catctgcccg ttccaccttt atcagcagtg taaaaggcaa ggggtaatta cgccccacag
     8101 tgctgatttt gcaacaactg gtgcgtctcc tggcgcacct ttttttatgc ttcattcctg
     8161 ggatatgagc gattttttat agtaactcac ttcttcttca ctaagaatat ccattatctc
     8221 aatgccttat cagagattct tttcctttcg ccggtagtgt ctggacgctc aggctacttt
     8281 tccaggttat tttatttctg ttatgcagag gttttatgat aagtcatatc ccaaattctg
     8341 gcggcaataa ctctttgatg aaacatga
//
LOCUS       DAZFAH010000185         8334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFAH010000185 DAZFAH010000000
VERSION     DAZFAH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.187"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..239
                     /locus_tag="ACHJWB_003948"
     CDS             <1..239
                     /locus_tag="ACHJWB_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315497.1"
                     /translation="SVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNL
                     TPETLFGSKSQDKHALFPIFTASWRATRIKNKGI"
     gene            462..959
                     /gene="ubiC"
                     /locus_tag="ACHJWB_003949"
     CDS             462..959
                     /gene="ubiC"
                     /locus_tag="ACHJWB_003949"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4315498.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            972..1844
                     /gene="ubiA"
                     /locus_tag="ACHJWB_003950"
     CDS             972..1844
                     /gene="ubiA"
                     /locus_tag="ACHJWB_003950"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4315499.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDQLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1999..4422)
                     /gene="plsB"
                     /locus_tag="ACHJWB_003951"
     CDS             complement(1999..4422)
                     /gene="plsB"
                     /locus_tag="ACHJWB_003951"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4315500.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4593..4961
                     /gene="dgkA"
                     /locus_tag="ACHJWB_003952"
     CDS             4593..4961
                     /gene="dgkA"
                     /locus_tag="ACHJWB_003952"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4315501.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5071..5679
                     /gene="lexA"
                     /locus_tag="ACHJWB_003953"
     CDS             5071..5679
                     /gene="lexA"
                     /locus_tag="ACHJWB_003953"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4315502.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5752..7077
                     /gene="dinF"
                     /locus_tag="ACHJWB_003954"
     CDS             5752..7077
                     /gene="dinF"
                     /locus_tag="ACHJWB_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4315503.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEILKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7193..7402
                     /locus_tag="ACHJWB_003955"
     CDS             7193..7402
                     /locus_tag="ACHJWB_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4315504.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7444..7959)
                     /gene="zur"
                     /locus_tag="ACHJWB_003956"
     CDS             complement(7444..7959)
                     /gene="zur"
                     /locus_tag="ACHJWB_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4315505.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1908 a         2029 c         2263 g         2134 t
ORIGIN      
        1 cctctgtcga tgatgggcct gttatggtgg taagctccca gcgcttacat gatatgttga
       61 atcctacaaa agataccaat tggaattcca cgtatatcta caaatccaga catgagatat
      121 tgcctgttaa tcttactccg gaaacacttt tcggctccaa atctcaggat aaacatgcac
      181 ttttccccat ttttactgcg agttggcgag ctactcgtat aaagaataaa ggtatttaag
      241 taaaggaaaa catcaccatt cctggcatca tgggcggtga tgcccctatg gttgccctcg
      301 ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt
      361 tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta aacacttttc
      421 tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa
      481 cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggagccg caactgctcg
      541 actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga aaaacggtga
      601 gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aattcccgaa gaactgccgc
      661 tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac
      721 cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt
      781 tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt cacatcatcg acattaaccc
      841 gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat
      901 taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag
      961 aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa
     1021 tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt
     1081 gggtggcgac accgggcgtt cctcagctct ggatcctggc ggtgtttgtc gcgggtgtct
     1141 ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag tttgatggtc
     1201 atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag aaagaggcgc
     1261 gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg ctgaatacga
     1321 tgactattct gttgtcgatt gccgcgctgg cgctggcgtg ggtataccca tttatgaagc
     1381 ggtataccca tctaccgcaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg
     1441 cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc ctcgccaata
     1501 ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat gatgatgtga
     1561 agattggcat taaatccacg gcaatcctgt tcggccaata cgatcaattg attattggta
     1621 ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat ggcttaggct
     1681 ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga
     1741 ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc
     1801 tggtactatt tttagggctg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg
     1861 gatgatcatc cggctttctt ctgggttgcc tgatgcgcgg cgcttctcag gcctacacaa
     1921 cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt tttcgccgca
     1981 tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac
     2041 gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg ccggttcggc
     2101 atcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag aactgaacac
     2161 cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac gttgcgcgac
     2221 ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac tcaacaacca
     2281 gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagcgt
     2341 gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg
     2401 ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat cccagcgcag
     2461 gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg
     2521 ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg
     2581 cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca gaatgatgat
     2641 gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag
     2701 ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt tgcgcatcag
     2761 atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga
     2821 tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg
     2881 taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg gacgcaccgc
     2941 ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa
     3001 cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc
     3061 gcgcagcatc tgcggcaggc tctctttctc tttcgtcgca ccgcgcagtt ctttggcgta
     3121 agtacccact tccatgacgt gctcataacc gatgtagatc ggaatcagcg taatcggacg
     3181 cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag
     3241 caaacgcccc gtacgggaac gaccgccttc cacgaagtac tcgacggaat aaccacggct
     3301 gaacagttcg ccgagatact cccggaaaac ggtagaataa agtttattgc ctttaaacgt
     3361 acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaaattcag
     3421 gttgatcccg gcggcgatat gcggcggcac cagcccctgg tgatacagca cgtaagaaag
     3481 tagcaggtag tccatgtgac tgcggtggca aggcacatac actagctcat gaccgtcgtg
     3541 ggccagctgg cgaacgcgct cagcgttatg gacgttgatg ccctggtaaa gtcggttcca
     3601 ggtgaagccc agaatacgat cagtcaggcg gatcatctcg taagagaaat tcgccgcaat
     3661 ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg
     3721 cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat taaacagatc
     3781 ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa agtgcatacg
     3841 cgccacgcgc gccagtttct gagcgatagt tttatccgtg ccgtgttcat ccgccatacg
     3901 gcgcagcgac actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacggc
     3961 gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc ctttttcacg
     4021 ccccggcgca cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt
     4081 gctgcggtgc aaatcgagat agtcgtggaa tagcttaata gactcttctt tcggcgtgta
     4141 ataggtgaac acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc
     4201 gatttccagc ggctctaacg ggtcaggcaa gtcatgtgcc agacactggg cgcgcaacgt
     4261 cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag aggtatccag
     4321 ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg
     4381 taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct
     4441 ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc
     4501 cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat
     4561 attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc
     4621 gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag
     4681 cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcattgcc tgctggctgg
     4741 atgtggacgc gattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg
     4801 aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa taccatgagc
     4861 tttccggacg cgcaaaagat atgggatccg ccgcggtgct gattgcgatt atcgtcgccg
     4921 tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag aattcgataa
     4981 atctctggtt tattgtgcag tttatggttc caaaatcgcc tttcgctgta tatactcaca
     5041 gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag gcaacaagag
     5101 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     5161 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     5221 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
     5281 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg
     5341 caacagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc gaatgctgat
     5401 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggcgacttg
     5461 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     5521 gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa
     5581 aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac cattgaaggg
     5641 ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga gaccgcgatg
     5701 ccgcctggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg catggcattc
     5761 ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt ctccaatatc
     5821 accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga tagcccggtt
     5881 tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat gctgttgctg
     5941 tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa aaatcctcag
     6001 gcattagccc gtgcgctggt gcaaccgttg ctgttggcat tgggggctgg ggcgttaatt
     6061 gcgctgctgc gtacacctat tatcgatctg gcgctgcata ttgttggcgg tagtgaagca
     6121 gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc ggcgtcgctg
     6181 gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt
     6241 ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt gatggggctg
     6301 catatgaacg tgcagggcgc ggcgctggcg acggttattg cggaatatgc aacattgctg
     6361 attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg cgaaatactg
     6421 aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg
     6481 cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc gcgactgggg
     6541 agtgacatta tcgctgttaa cgcggttctg atgacgctac tcacctttac cgcctatgcg
     6601 cttgatggtt ttgcctacgc ggttgaagcg cactccggtc aggcatacgg tgcgcgcgac
     6661 ggtagccagt tgctggatgt atggcgggca gcgtgccgcc agtcggggat tgtagcgtta
     6721 ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct gacgtcgtta
     6781 acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt gccgttggtt
     6841 ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc cgccgaaatg
     6901 cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac gctgccgtgg
     6961 ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg cgggctttct
     7021 ctggcggcta tctggcggcg tcactggcgc aacggtacct ggtttgccgc aacgtgacgg
     7081 ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa tctcacgttc
     7141 agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga ttatgaataa
     7201 agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa
     7261 actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg tcggtaaaat
     7321 ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac
     7381 ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag gatgtacgcc
     7441 ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact gttcaggatg
     7501 acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa tcacattatg
     7561 ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca cgccttctgc
     7621 acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg tatgcgtggg
     7681 ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat gcacaaaacc
     7741 ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt gcggttcagc
     7801 ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca gactcatcag
     7861 gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc agattttttc
     7921 agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca aagtgggggt
     7981 taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt attgttgccg
     8041 taatgttgat tttctgtttt gtaggtaagg tgttgtgttg ccttgtcgta ccattatcaa
     8101 cacgataata attaataatt catttttaaa taaattgctt caagttaact cgctatttat
     8161 ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt tgatagtgac
     8221 cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga tgaatcatgt
     8281 tgaagataat acccggtgca actggatact ttaataaaac actcaattca aatc
//
LOCUS       DAZFAH010000186         8325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFAH010000186 DAZFAH010000000
VERSION     DAZFAH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.188"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..625
                     /gene="yebB"
                     /locus_tag="ACHJWB_003957"
                     /pseudo
     CDS             23..625
                     /gene="yebB"
                     /locus_tag="ACHJWB_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
     gene            complement(627..1148)
                     /gene="ruvC"
                     /locus_tag="ACHJWB_003958"
     CDS             complement(627..1148)
                     /gene="ruvC"
                     /locus_tag="ACHJWB_003958"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4315506.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1183..1923)
                     /gene="yebC"
                     /locus_tag="ACHJWB_003959"
     CDS             complement(1183..1923)
                     /gene="yebC"
                     /locus_tag="ACHJWB_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4315507.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1952..2404)
                     /gene="nudB"
                     /locus_tag="ACHJWB_003960"
     CDS             complement(1952..2404)
                     /gene="nudB"
                     /locus_tag="ACHJWB_003960"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4315508.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2522..4294)
                     /gene="aspS"
                     /locus_tag="ACHJWB_003961"
     CDS             complement(2522..4294)
                     /gene="aspS"
                     /locus_tag="ACHJWB_003961"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4315509.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4604..5170
                     /gene="yecD"
                     /locus_tag="ACHJWB_003962"
     CDS             4604..5170
                     /gene="yecD"
                     /locus_tag="ACHJWB_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4315510.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNHAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPFPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5167..5985
                     /gene="yecE"
                     /locus_tag="ACHJWB_003963"
     CDS             5167..5985
                     /gene="yecE"
                     /locus_tag="ACHJWB_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4315511.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6038..6433
                     /gene="yecN"
                     /locus_tag="ACHJWB_003964"
     CDS             6038..6433
                     /gene="yecN"
                     /locus_tag="ACHJWB_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4315512.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6474..7217
                     /gene="cmoA"
                     /locus_tag="ACHJWB_003965"
     CDS             6474..7217
                     /gene="cmoA"
                     /locus_tag="ACHJWB_003965"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4315513.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYHGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7214..8185
                     /gene="cmoB"
                     /locus_tag="ACHJWB_003966"
     CDS             7214..8185
                     /gene="cmoB"
                     /locus_tag="ACHJWB_003966"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4315514.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
BASE COUNT         1891 a         2191 c         2102 g         2141 t
ORIGIN      
        1 tctatttcaa tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga
       61 tatcgtcttt acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg
      121 ctggagtaat cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga
      181 aagccgggtt cccctttcaa ccatcaccac gctatctcgt tttattaaac gttcgtctaa
      241 tcaacgctat gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat
      301 tgttgaacag gttccctccc ggctacgcaa actttaccac acgggtttta aatacgaatc
      361 ttcgcgccag ttctgttcaa aatttgtttt tgatatttat aaagaagcgc tatgtattcc
      421 ggtgggtgaa atagagacgt ttggagaatt gttaaatagc aatccgaatg caaaactcac
      481 tttctggaaa ttctggttcc tgggttctat tccgtgggag cgtaaaaccg tcacgccagc
      541 cagtttgtga catcatccgg gtttggtgtt gattcacgcg gagggagttg aaacgcctca
      601 gcctgaactg accgaggcgg tataacttaa cgcagtcgcc ctctcgccag gttcagccgc
      661 gattcgctca tctgcattgc attctggctg acgtgacaat gggtaatcgc gatagctaac
      721 gcatcggcgg catccgcctg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc
      781 tgcacctgac ttttttcggc actaccaata cctaccaccg tttgctttac ctgacgtgcc
      841 gcatattcaa ataccggcaa ttcctgattc accgccgcca caatcgccac gccgcgcgcc
      901 tgccccagtt tcagagccga gtcggcattt ttggccataa agacttgttc aatggcgaaa
      961 taatcaggct ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga
     1021 gacggtaaat catccacttt ggtgcgtatg catccgctac ccaggtagga cagttgccta
     1081 cctacctggc ggatgacgcc gtagccggtc acgcgcgaac ccgggtcaat gccgagaata
     1141 atagccatca cgcgtctccg ttttgctgtt tagcaggcct catcagagag tcgctgcgac
     1201 ctcatcagag atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat
     1261 atcgatcaga cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt tggtggacgg
     1321 gatcatggaa acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac
     1381 tttacccatt tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat atgtcacaac
     1441 gtcttcagca cctgcttcca gagctgcttc catgatggtg tcttcgtcgc ctttctcgaa
     1501 ggagatcacg ccttttttgc tgaacagata ggctacggaa ccgtcagtgc cgaggttacc
     1561 gccacatttg ctgaatgcgt gacgcacttc agcaacggta cggttgcggt tgtcagacag
     1621 acattcaatc atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat
     1681 gtttgcatca tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt
     1741 catgttgtta gacagtgctt tatccaccgc cgcacgcaga cgcgggttag cgtccggatc
     1801 gccaccgccc agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc
     1861 gcgcttagca tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc
     1921 cataaaaata tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa
     1981 tcgcctgccg gttgctccag gacttagtga gcgccgccgc agcaggcgca tcaagccact
     2041 tgtaagccag atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc
     2101 atgattccgt attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt
     2161 caaactctac cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg
     2221 tgacctcttc ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc
     2281 tgccggttac cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt
     2341 tcgtatcttg tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct
     2401 tcacgctgcc tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca
     2461 tgggagttca ctcagttgcc gcctcgatgc aacgcgaatg atttcgtgta tttgagtcat
     2521 atcagttatt ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt
     2581 tagcaaagct cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga
     2641 taacgtcacg gatattgtcg gtgccggtca gcagcatggt cagacggtca agaccgaatg
     2701 ccagacctgc gtgcggcgga gtaccatatt tcagggcgtc gagcaggaag ccgaatttct
     2761 cgcgctgttc ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat
     2821 tatggatacg tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt
     2881 tcgccaccgc attttccggt gcagctttca gctctgcagc cgtcatgtct ttcggtgagg
     2941 tgaacggatg gtgcattgcc gtcaggccgc cttctccgtc gtcttcaaac atcgggaagt
     3001 cgataaccca cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt
     3061 tcaggcgcag tgcgcccatc gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa
     3121 tcatatcgcc atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa
     3181 ggaacttcgc taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttaa
     3241 tgtaagccag acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct
     3301 tacgggtcag cgatgcgcca cccggaacgc gcagagccgc tacgcgacct ttcggatcgt
     3361 tcgccggacc tgcaaatact gcaaactcaa cagatttcag cagatcagca acgtcggtca
     3421 gttccatcgg gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa
     3481 aggtcattac cgggaaatcg cccagatcca cgcccttcac ttccagccac agatggcgca
     3541 ccagcgcttc catcacttca cgcacttgcg gggcggtcat gaaagaagtt tccacatcga
     3601 tctgagtaaa ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga
     3661 tctgatagta gcggtcaaag ccggacatca tcagcagctg tttgaacaac tgtggggatt
     3721 gcggcagtgc gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc
     3781 cttccggcgt ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca
     3841 taaaacggcg caccaggctg gtgattttag cgcgggtttt caggcgctga gccatttccg
     3901 ggcgacgcag gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag
     3961 agtcaagcgg cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcga
     4021 tttcgccggt cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg
     4081 tgacctgaat gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat
     4141 ccggatcgaa aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc
     4201 taccaagatc acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg
     4261 acaaacggag ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg
     4321 cggatgcccc ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa
     4381 tgaaaaaagg cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct
     4441 ggaacgcctc gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat
     4501 aaaggtgttg cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc
     4561 gcattaaggt gtgaggcacg attttgtttt ttcaggagtc agcatgcttg aacttaatgc
     4621 taaaaccact gcgttggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg
     4681 tccacatact gccgatgagg tagttaatca cgccgggaag ctggcggcga aatttcgcgc
     4741 cagcggtcag cccgtgtttc tggtgcgcgt tggctggtct gccgatcacg ccgaagcatt
     4801 aaaacagccg gttgatgccc ccttccccgc taaagtgttg cccgaaaact ggtggcaaca
     4861 tccagctgca ttaggtgcaa ctgacagtga tatcgaaatc atcaaacgtc agtggggtgc
     4921 gttttacggt acggatctgg agttgcaact gcgccgccga ggtatcgata caatagtgtt
     4981 atgtgggatc tcgaccaata tcggtgttga atccaccgcc cgcaatgcct gggaacttgg
     5041 ctttaatctg gtgattgccg aagacgcctg tagtgccgcc agcgccgagc aacacaataa
     5101 cagcattaat catatctacc cgcgcatcgc ccgtgtgcgt agcgtggaag agatccttca
     5161 cgcgttatga tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg
     5221 atcaccagcc ttgaagagta tgcccgccac tttaactgcg tggagggcaa caccacgctt
     5281 tacgccctgc cgaaacccga ggttgtcctg cgctggcgtg aacagaccac agatgacttc
     5341 cgcttctgtt ttaagtttcc ggcgaccatt tcgcatcagg cagcattacg acattgcgat
     5401 gatttagtga ctgaattttt gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac
     5461 tggctgcaac tgcctgccac attcggccca cgggagctgc ctgcgctttg gcattttctc
     5521 gattctctcc ctggcgaatt taattatggt gtggaagtcc gccatccaca gtttttcgcc
     5581 aaaggggaag aggaacaaac gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg
     5641 attttagaca gccgccctgt tcatgcagca cgtccacaca gtgaagctat tcgcgacgct
     5701 caacgaaaaa aacctaaagt tccggtacat gctgtactga cggcgacaaa tccgctgatt
     5761 cgttttatcg gtagtgatga tatgacgcaa aaccgggaat tatttcaggt ctggttacag
     5821 aaattagcgc agtggcatca gaccactacg ccttatcttt ttttacatac gccagacatt
     5881 gcccaggccc cggaactggt acataccctg tgggaagact tacgtaaaac gcttccagag
     5941 atcggagcag ttccggctat tccacagcaa tcttctcttt tctgaatttg ccacctatca
     6001 tagacaggtg ccatcggcca ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt
     6061 tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta
     6121 ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa agcgctattc gcattcatgg
     6181 taacgcggtg gaatatattc ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg
     6241 cgcagaaacc tggatggtgc atatttgcgg catcgttttg cttgctggtc gtctgatgca
     6301 ttattacggt tttcatcacc gtctgtttcg ctggcgacgt tccggcatga gcgccacctg
     6361 gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat atgccctggg agttggtttt
     6421 ctccctgcgt tagcgcacaa tacgccactt tctttttccc ggatttttac gttatgtctc
     6481 accgcgacac gctattttct gcccctatcg ccagactggg cgactggacc tttgatgaac
     6541 gggtagctga agtcttcccg gatatgatcc agcgttccgt tcccggctat tccaatatta
     6601 tttccatgat tggtatgtta gccgagcgct tcgttcaacc tggtacgcag gtttacgatc
     6661 tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg caacattcat catgataatt
     6721 gcaaaattat tgccatcgac aactccccgg cgatgattga acgctgccgt cgtcatattg
     6781 acgcctataa agccccgacg ccagtagacg ttattgaagg tgatattcgc gatatcgcca
     6841 ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca attcctggaa ccttccgagc
     6901 gccaggcgtt actggataaa atttatcatg ggctgaaccc gggcggcgcg ctggtgcttt
     6961 cggaaaaatt cagtttcgaa gatgccaagg ttggtgaact gctgttcaac atgcaccacg
     7021 actttaaacg tgccaacggt tacagcgaac tggagatcag ccagaagcgc agcatgctgg
     7081 aaaacgtgat gctgaccgat tccgtggaaa cacataaagc acgcctgcat aacgccggtt
     7141 ttgagcatag cgagttgtgg ttccagtgct ttaactttgg ttcactggtg gcattaaaag
     7201 cagaggacgc tgcatgatcg actttggtaa cttttattct ctgattgcca aaaatcatct
     7261 ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac tggcagcgcg agcagcagca
     7321 cgggctgttt aagcagtggt ccaacgcggt ggaatttctg cctgaaatta aaccgtatcg
     7381 tctggattta ttgcatagcg taaccgccga aagcgaagag ccactgagcg ccgggcagat
     7441 taaacgcatt gaaacgctta tgcgcaacct gatgccatgg cgcaaagggc cgttctcact
     7501 gtatggcgtc aacatcgata ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc
     7561 ccatctttct gatttaaccg gacgcaccat tctggatgtc ggctgcggca gcggttatca
     7621 catgtggcgc atgattggcg caggcgcgca cctcgcggtg gggatcgacc ctacgcaact
     7681 gtttctgtgc cagtttgaag cggtgcgtaa actactgggt aatgatcagc gcgcgcattt
     7741 gttaccgtta ggtattgaac aacttccggc actgaaagcc tttgataccg tcttttcgat
     7801 gggcgtgctt tatcatcgtc gttcaccgct ggagcatctc tggcagttga aagatcaact
     7861 ggtcaatgaa ggtgaactgg tgcttgaaac gctggttatt gatggtgatg agaacacggt
     7921 gctggtgccg ggcgatcgtt acgcgcaaat gcgtaatgtc tatttcatcc cttcggcgct
     7981 ggcgctaaaa aactggctga agaagtgtgg ttttgtagat atccgcattg cggatgtgag
     8041 cgttaccacc acagaagagc agcgacgcac cgaatggatg gttaccgaat cgctggccga
     8101 tttcctcgac ccacatgatc cgggtaaaac ggtggaaggt tatccggcac ctaaacgcgc
     8161 ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc
     8221 cttatccggc ctacaaaacc ttgctaattc aatatattgc agggactatg tagatactgt
     8281 tatggcctgc aagttttttc ctggtccggt ttcccggatt gcagg
//
LOCUS       DAZFAH010000187         8299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFAH010000187 DAZFAH010000000
VERSION     DAZFAH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.189"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..2883)
                     /locus_tag="ACHJWB_003967"
     CDS             complement(13..2883)
                     /locus_tag="ACHJWB_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4315515.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGPPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2880..3659)
                     /gene="ygfM"
                     /locus_tag="ACHJWB_003968"
     CDS             complement(2880..3659)
                     /gene="ygfM"
                     /locus_tag="ACHJWB_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4315516.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3710..5038)
                     /gene="ssnA"
                     /locus_tag="ACHJWB_003969"
     CDS             complement(3710..5038)
                     /gene="ssnA"
                     /locus_tag="ACHJWB_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4315517.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5041..8139)
                     /gene="ygfK"
                     /locus_tag="ACHJWB_003970"
     CDS             complement(5041..8139)
                     /gene="ygfK"
                     /locus_tag="ACHJWB_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4315518.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSPGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVMKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         1904 a         2169 c         2186 g         2040 t
ORIGIN      
        1 gccatttatt atttatattt tttccagcgc agtgagtatt ttctccggtg tgaaatgcca
       61 ttcgcgtaac cagatgccgc atgcatcgtg aattgcggta gcaatcgccg gagctgcgcc
      121 atttacaccg atttccgaga tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg
      181 caccagcaca gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg
      241 taaatcacgc gttaacgggg gaccttcggc gtcataaatg atctcttcgc tcatactgtg
      301 gccgatagct cgcagggtag caccgtagat ttgtcccaat gctaattccg ggttgaccgg
      361 tgtaccgcag tccagcaagg cgtagaattt atccaggcgg atttcacccg tacgcgtgtt
      421 gacggcaact tcagcgaagt ttgcgccata cgggaaggcg aaatcaggcg tgatataact
      481 gccagttccc accagtgaac caaagccggt gccggtttcg cctttatgag caatatcccc
      541 gaagctgact tcgcctttct tgccgcgcac gacgcccggc gttgccagtt gaacatctgc
      601 cactgcctca cccaacattt gcgcgccgtg gaacagaatt ttctcccgca gattttccgc
      661 tgccaaacgc gccgcgttac ctgagaagca agtaccggac gaggcatatg cgcctttatc
      721 aaacaacgca tgatcggtat caccggagat aacatgcacg tcctgcggtg ggcagtgcag
      781 cacttctgct gccagtttcg tcactacagt atccagacca gtaccaatat ccgcaccgcc
      841 agaatgaacg ataaaggtgc cgtccgattc cagtttgatc atgcagttag cctgatcgat
      901 atccgggata cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcacc
      961 attttgcggt ttcggtgacg accattggat catctcgcga ccctgacgca ggatctcttc
     1021 cagtgcacag ctggcggcgg aaggaacgga ggtcggcgct ttaccttcac cgattgcacc
     1081 gagaattttc agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc
     1141 gatctgtaac tgttcagcca gttccgctaa tgccatggtg atagcgaagt tacctttcgg
     1201 tgcgccataa ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg
     1261 gaaatcgacg ttatcgcacg gatataacgg cagcgacagc gccggtccgt tacacggtac
     1321 ggtgagtgag tggttgccgt aagggccagt gttggcgcgg aaatccatct tcactgccgt
     1381 caggcgacca tcttttttcg ctcccagttt gacggtgact ttcgcgacgt gacgagaggt
     1441 gttagcaata aactcttctt cacgggtgta gcggaacagt accggacgcc cggtcacgca
     1501 ggttgcccag gcgcacactt cttccagcag gatgtcctgt ttggaaccaa aaccgccgcc
     1561 aactcgctct ttaatgacat gaactttatg ctgtttcatg cccacgaggc gcgcgacctg
     1621 gcggcgtaag tgccatggta cctgggtgga ggcgtggata accagacgat cgccgtccat
     1681 ccgggtaaag cagatatgtg tttcagtcgg gcactgctgt gcctgcgttg agttataggt
     1741 tcgctcaatg atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg
     1801 aatactggcg gcgatatttt tgcgagggcg agaaccgatc gggaagttga tgatcatatg
     1861 ctcgccgcgc tgggctgcat ggctgttatc gtcttccaga gtatctggcg caccagcaac
     1921 atacaccacc ggttcatcgt gcacgacagg cgcatcttcc gccattgctt cgtcgatcga
     1981 cattaccggc ttaagcactt catattcaac gtcgatgagc ttcaatgctt cgagcgcaat
     2041 ttcttcactt tctgcgacga ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc
     2101 gaacatacgg cggtcaagcg gtgacggttc cggtgcgctc tgaccacccg gggtatagta
     2161 gatatccggg caattcaggt gagtaataac gtgaacgacg cccggtaagg cttcagcttt
     2221 gctgacatcc agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac
     2281 gcaggcgtca gccgttacgc ggtcttcaac atagcagggt ttcgcctgca ccattttcgc
     2341 ggcatcagtt ttaggataat gcttaccaat gacttctagg tcgtcacgga aagtcggagc
     2401 gatatcaatg gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata
     2461 gtattgctgc cagccagcat cgcggctgaa caaaccagaa agcgcgtcgt cgatctcttc
     2521 gcgggtaggt gcggcgatgc gatcgagaag atcggtgata atcagagctg cagctggatc
     2581 gttataacca gactgcacca cgccaacatc aaccattgcc tgttgaacca gacttaactc
     2641 gttccatttg cccagagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcaat
     2701 aagcaaggac gcgttaacga tattaccgtt aaagattatt gcgtcagaac cggcaaaccc
     2761 gaaaccatca tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac
     2821 gttttcgcct ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat
     2881 catacggctt cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc
     2941 gtgatatagc gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg
     3001 gcgacagcct gttcaagtgc attgccttcc agattttgtt tttcgacatc atgcagacgc
     3061 agtgctttac tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgtcattgca
     3121 acggctgccg tcacgacggt taaacctgcc tgagaacggc taattttgcg ggtcgcacag
     3181 gtgcgatacg gatctttaat gataatttcg gttagcaggc gatcgcatgg gcaggccagg
     3241 tagtcctcga ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cagtgccaac
     3301 aggacgggaa gcagcaccga ctcttcctgg cgggcggcga tttcaccacc aatggtcgac
     3361 tgattacgaa catggcgtga gtaaacaaaa ccgagggctt cacgcagcgc tgcaggaata
     3421 aatcgcgcat cacgcagtgg ctgcaagcga gacattgcgc caatccgcag tgcaccgtta
     3481 tcccagtcaa cccagtccag ttccagatcc tgtaaggaaa tggcaatctt tttatcggta
     3541 cgggttggtg tagcattgag tttgctgccc ccggcgaacc agacggcttc atcctggtag
     3601 cggcgcttca gttccagcgc ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc
     3661 ttctttcctt atttaaccct cttccctgcg ggaagagggc atctgtcatt tatgccagcg
     3721 catccatccg acgccacata ctggcagcgg cttttctggc ttgtgcataa atggaatcgc
     3781 aatcgaagtt aaactgacgg tcttcataga ccatcacacc attgaccatt acgctgtgaa
     3841 cgctgcctga gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg
     3901 tcggcgagtt gtaatcgcaa atggttaaat cagctttgta accggcttcc agaagcccaa
     3961 atttcgcgcc aaagttgcgg ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac
     4021 tgtcaggcca cagcggacca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt
     4081 caaacatgtc cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt
     4141 gatggttgta gccgacatgg ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat
     4201 cgcgctgatt gagtagggcg atgtcatctt tcgacaagta cagcccatga gcgaccagcg
     4261 ttttgctgtc gatgagatcg aattgcgcca gtcgtgccag caggtctttg ccgtaccagt
     4321 ggtgactgta ggaaacgtcg taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg
     4381 tggctttcac ggcttcatgc agcatctcca gaccggcatc cggcacggta aacggcgcgt
     4441 gagcaccgat atgtgcttcc accagatacg gctcgcttgt cgctttcttc gcctcatcaa
     4501 tcaaacgggc gaaacggatg ttttcttcta caccttcctg caactctttg atgccgttgt
     4561 tacggtcagt agtttcaaaa caggtcattg cgcgcaggcc aacttttaaa aatgcgtcgc
     4621 gcaatgtgga gagcgacccg ccgatatacg ccggagaggc atggtgatcg ataaccgatg
     4681 tacatccgct cttaatcgct tccagggaac aaatcagtcc gctgtaatag agcgactctt
     4741 catcaagggc gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc
     4801 agggggcgat gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc
     4861 agacaattcc cggcatcaca atccgaccat gcatctcttt gtagctggca tcggggtagc
     4921 gttgcgtcag ggcatcgccg atagcgacaa tcacgtcatt ttcgatagcg atatcaacgc
     4981 cttcctgcac tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga
     5041 ttacacctcc acgcggccca gcagataatg atgatgctgg tggacatggc tgatgatgcg
     5101 gcacatatcg ttcagctccg gtggtacgtt gttaaactga ccttcgctgt cgatgtttaa
     5161 cacccagctt tggttattca gacgtactcg tacccggcaa tcttccacaa ggaagcctgg
     5221 gttgctgctg ttatcaaagt cttgcgccag gctgaagacg gtgattttgt ctttgtatgg
     5281 tttaccgttc cacggacaga actgagcgca gttgccgcat tcgttacagt aagcgtcgag
     5341 gtgcagcgtc tggaatctat tctggaagcc agggactgca atggagacgt tggcgcggtt
     5401 cgggcagaca tccacgcact tgctgcaaac gtagttacat tcaaggcagc gagcagcttc
     5461 ctgcgcgaca aacgcatcac gatcgtcact gttcaccagc gtgatagaga tatcgccttt
     5521 acgttgatag atttccgccg gattgacgtt gttccagtat ttattgttct ggtgggaacg
     5581 gatattttcc cgactaagga tggcatcggt cgcccgacgc gcggttccga cagcagcgac
     5641 aatggaggat ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgagtttc
     5701 gccattatgg tcgacgtctg gccagccgtt tttgtccagc ggtacgccca tcgcattcag
     5761 ggcttcagta tcctgctgtt caccgatggc ggtgatcaga ctgtctacat gcagtgttac
     5821 tgtttcgttg gtttcaaccg gacggcgacg acctttctca tcgggttcgc caagcgacat
     5881 aacgcgcaag gttaaggtgc catcagcatc gaaacattcc ggattattca ggaaacggaa
     5941 ctccacgccg tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tctcttgcag
     6001 tgaacgacgg taaacgatcg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc
     6061 gcagtccatt gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc
     6121 tgtacccttg ttgtattcac gcaggaaggg gagtgatttc cagacatttt ggttgtcacc
     6181 cgccagtttc acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc
     6241 ctgatttttt aactgctcaa cggttaaatc gggtgagcag ccatactcaa atttcacgcc
     6301 gtgagcggca acaaaatcga tatcgtgctg aattaactct gcaggaatac ggaactgagg
     6361 aatgatattt ttcatcacgc cgccagcatt ggcttcgcgt tcaaacagcg taaccggatg
     6421 gcccgctctg gcaaggaagt aacctgctgc cagacccgcc ggacctgcac caatcacggc
     6481 aactggatgg cgtgaaccag aaccggctgg tttgtgccag cgttgcttat attcatccca
     6541 acctttttcc agcgcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag
     6601 gcgggtacag ttgtattggc actggtgatc gcaaatatga ccggtaatgg cgggcagagc
     6661 gttgcgttgg tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat
     6721 gtattccgga atatcttgct tgatagcaca ggcagtaaca cagggggcaa cgtagcagtc
     6781 ggtcagcggc aggtcttctg ccacttcaat acgctcttct ggcttccagt gtttctgggt
     6841 gtattccatg gttaacgcat ctgctgccag tctgttcagt cgttcgacgt caacatggtc
     6901 aagcccccag gcgtcggagc cttccagctc gcgcatgcag gcacttaagc gcagatagcc
     6961 gcctggtttc agcaggtcgg ttgccatagt aatagggcga ataccagtat caaaaatatc
     7021 gcggatagtc agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc
     7081 gcgagagaga actgctgcaa cattgatgga gagcgggaac agcgcacggc ctgacatata
     7141 catctcttca ccaggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt
     7201 tacgccaaag ccgggtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag
     7261 tgcttgcgtc agcttgaggt cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa
     7321 accgcagaca tcgaggatct cacgaacacg cgcgtatccc agtaaggtcg ggttaagttt
     7381 cacaaaggtg ttgagccctt tttcttccag catgtagcgg caaatggctt cgatttcatg
     7441 cggaggacag ccgtgcatgg tggagagggt gacgccatgc accatactgg tggggatgcg
     7501 agcgggtaag gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcgag ctaaaaatgc
     7561 gtcatcctgg agtaatttat tcagcgtatc gcgatattga gcgaatttcg gatggtcaga
     7621 tgcgtccatc atattgtcga tgaactgttg catcggcggt tgcttaatac cttcgaggtt
     7681 gtaaccgacg ctcatattaa agatgaacga tttaccggaa tcagaaggct ggaacatcgc
     7741 ttcgagaagg tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt
     7801 aaattcggta gaccattcgg tgttaaagca ctcgtcttcg gcatcgatac agggcttttc
     7861 cagctccagg cggtcaagaa tttggacggt ttttagttcg atgaagcgtc cgccagtcag
     7921 ccaggaagtg acaatatttt gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg
     7981 agtggcacag gtttcaccga agacgctaac agttttacct tttacgggtg agtaaaactg
     8041 ttgctcggga ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa
     8101 aagttcctca aacggaatgg gacgcataat atcccccata accctctcct tagcttatac
     8161 agatatcatt gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat
     8221 cgcaccactc ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt
     8281 aagggctttt tgtgaatga
//
LOCUS       DAZFAH010000188         8287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFAH010000188 DAZFAH010000000
VERSION     DAZFAH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.190"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..1723)
                     /gene="glnS"
                     /locus_tag="ACHJWB_003971"
     CDS             complement(59..1723)
                     /gene="glnS"
                     /locus_tag="ACHJWB_003971"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4315519.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1926..3872)
                     /gene="nagE"
                     /locus_tag="ACHJWB_003972"
     CDS             complement(1926..3872)
                     /gene="nagE"
                     /locus_tag="ACHJWB_003972"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4315520.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4205..5005
                     /gene="nagB"
                     /locus_tag="ACHJWB_003973"
     CDS             4205..5005
                     /gene="nagB"
                     /locus_tag="ACHJWB_003973"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4315521.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5065..6213
                     /gene="nagA"
                     /locus_tag="ACHJWB_003974"
     CDS             5065..6213
                     /gene="nagA"
                     /locus_tag="ACHJWB_003974"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4315522.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6222..7442
                     /gene="nagC"
                     /locus_tag="ACHJWB_003975"
                     /gene_synonym="nagR"
     CDS             6222..7442
                     /gene="nagC"
                     /locus_tag="ACHJWB_003975"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4315523.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7490..8242
                     /gene="nagD"
                     /locus_tag="ACHJWB_003976"
     CDS             7490..8242
                     /gene="nagD"
                     /locus_tag="ACHJWB_003976"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4315524.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         2019 a         2183 c         2120 g         1965 t
ORIGIN      
        1 ttgctatttc ttgccggatg cgacgtaaat gcctcacccg gcctggtaga acttaaaatt
       61 actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat accggttttt
      121 ccgctgtgga atggcggctg tcgaggcaga agtaaccttc acgctcaaac tggaatgctt
      181 tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc accagcgatt
      241 ccgggttaat caccgacagg aaatcatccg cagcaccagg gttcggcacg ctgaacagac
      301 ggtcgtacag acggatttca accggcagcg catgtgccgc gctcacccag tgaataacac
      361 ctttgacttt acgaccatct gccggatctt tgcttaaggt gtcggcgtca taagtacaga
      421 agatggtggt gatattgcct tcggcatctt tctccacgcg ttcagcctta atgacgtaag
      481 cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg ttagcttctt
      541 cgcggaaatc ggcgcgatcg atccaaatct caccgctaaa cggcacctga cgactcccca
      601 tttccggttt gttcggatgg ttcggcatgg tgaccatttc gccttcgccc tgatagtttt
      661 cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt tcgttgagat
      721 cttcacggat gcaggattcc agcgatgcca tctcaatggt gttgtcctgc ttggtcacgc
      781 cgatgcgttt gcagaactca cgaatagaag cagcagtgta accacgacga cgcagaccgg
      841 aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc accagcaggt
      901 tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat tcgtactggc
      961 gcgggtgaac aggaatggtg atgttgtcca gcacccagtc gtacaggcga cggttgtcct
     1021 ggaactcaag cgtacacaga gagtgcgtaa taccttccag ggcatcgctg atgcagtggg
     1081 tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt tcagcaaatt
     1141 taatacggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg tcgattttcg
     1201 cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg aacagcgcca
     1261 ggttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggttgc gtcagggtgc
     1321 cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg cctttattga
     1381 tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa cggacgttac
     1441 cagaccagtg aaaacctaac cactctacgt cgtttttgat cgactcaaca tactcgatat
     1501 cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta tagtcctggg
     1561 cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag ccattcggct
     1621 ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct tcatcgatga
     1681 tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat tcctcaaagc
     1741 gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa ctgttagttt
     1801 tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa aagcggcaga
     1861 gaagatctcc accgcttgtc attgttggat gcgacgctca agcgtcgcat caggcataaa
     1921 gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg gccctgagct
     1981 ttaatgatca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct aatcatcgag
     2041 cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc tgcgcttacc
     2101 tgcgcaccct cttccaccag acgtttaaag cctttacctt ccagcgctac ggtgtcgata
     2161 cccatatgga cgacgatctc cgcgcctttt tcggtttcca ggcagaacgc gtggttggtg
     2221 ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc tgtcggtttc
     2281 accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac ctgatccagt
     2341 gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc gtttggtaca
     2401 gcctgcggtt ttgctacagg cgcggcagtt gccggagttg cttcagctga cgcagcggct
     2461 accggaccac gggcaacgac tttcttcatc gcatcgccga tggattctgc tttcgcgcca
     2521 acaatcacct gaatagtctg tttgttcagt ttcactacac cagaagcacc cagacgttta
     2581 cacatcgtat cgttgacgcg ggcagagtca gccacggtca ggcgcagacg ggtgatacag
     2641 gcgtcaattg ctttcaggtt gtcagtgccg ccaaccgcag caatatagtt ggtcgccagt
     2701 tgattgaggc cttcttcagt gttgctgttg gcttcttcag taacgatctc gtcttcttta
     2761 tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa caccacgaag
     2821 tagatagcga agaagacaac acccataacc agcagcatcc agacgttctg gctggcggcc
     2881 ggcaggttat acatcaacgc gtagtcgata gcccccgcag agaaagagaa gccagcatgg
     2941 atacccagca gtgttgccac aaacaggctg ataccggtca gcagtgcgtg caggaggtac
     3001 agcagcggtg caaggaacat gaacaggaat tccagcggct cagtcacacc ggtcaggaac
     3061 gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc tttcggtgct
     3121 gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcataatcgg gaagaagccg
     3181 gacatgaaca tccccgcggt gccgtcaccg gcatagaagc ggttgatatc accgtggaaa
     3241 accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt gttcagcacc
     3301 tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc ggaacccagc
     3361 gcgcccgcag aaacgatcca ctcgcctcct gcatggatag cgtgctgtac cggcggccag
     3421 acgtaaccaa aaatggccgc cagcaccagg cagaagaagc cggtggcaat cggcacaaag
     3481 cgtttgccgc cgaagaaact caggaagtcc ggcagtttaa tatcggacca acggttatag
     3541 gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt aatttctggg
     3601 ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc cagcgccgcc
     3661 gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat taatgcgagg
     3721 ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa atctggctga
     3781 ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga tagggagctg taacgcccta
     3841 ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga accctatttg
     3901 gctcgtttaa agccgtattt ttattttgct gcaaattgta ctgccgatgt tctgtaatca
     3961 gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg
     4021 cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt tactggctaa
     4081 agcagaaaac ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa
     4141 tccaggttac gcttaaagat gcctaatccg ccaacggctt acattttact tattgaggtg
     4201 aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg ctgctcgcca
     4261 tatcgtcaat cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg ttctgggcct
     4321 gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg
     4381 ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg gtctgccgaa
     4441 agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc acgttgatat
     4501 tccagcagaa aacatcaacc ttctcaacgg caacgccccg gatatcgacg ccgaatgccg
     4561 ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg
     4621 taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat
     4681 caaaaccttg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata acgatgtcaa
     4741 tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ttgctggatg ccgaagaagt
     4801 gatgattctg gtgctgggta gccagaaagc actggcactg caggctgcag ttgaaggttg
     4861 cgtgaaccac atgtggacca tcagctgtct gcaactgcat ccaaaagcga tcatggtgtg
     4921 cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca atgaattaga
     4981 agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggctccttg ctcagggcaa
     5041 tattttttaa aatcgggggt cagaatgtat gcattaaccc agggccggat ctttaccggc
     5101 cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt
     5161 ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc cattctctcc
     5221 cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa cgacaccgct
     5281 gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa atcaggctgt
     5341 actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc
     5401 gttatgcgcg agtacctggc aaaacatccg aatcaggcgt taggtctgca tctggaaggt
     5461 ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg taagcctgat
     5521 gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt gaccctggca
     5581 ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat tgtggtttct
     5641 gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc
     5701 tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg
     5761 ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg cctgcatgtt
     5821 gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg tctggttacc
     5881 gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg taaaacaata
     5941 tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc atccttaacc
     6001 atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga tgaagtgctg
     6061 cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct cggcacactc
     6121 gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat caccaagacc
     6181 atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca ggcggacaag
     6241 ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga
     6301 ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag cttgcccccg
     6361 ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc
     6421 agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc cgcaatttcc
     6481 acgcaatcgg cgtacggctt ggtcgtcacg acgccaccat caccctgttt gatctcagca
     6541 gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcaa acgctggaac
     6601 atgccctgtt gaatgccatt gctcagttta ttgatagcta ccagcgcaaa ctgcgcgagc
     6661 tgatcgcgat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc aaaattcatt
     6721 acatgccgca tattcaggta gaaaactggg ggctggtaga agctctggaa gaacgtttta
     6781 aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag cactacttcg
     6841 gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga accggggccg
     6901 ggattatctc taacgggcgc atttttattg gccgcaacgg taacgtcggt gaaattggcc
     6961 atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc tgcctggaaa
     7021 ctatcgccgc caacgctgcc attgaacaac gggtgttgaa tctgttaaag cagggctacc
     7081 agagccgcgt gccgctggac gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag
     7141 gcgatagtct ggcgtcggaa gtaattgagt atgtcggtcg tcatctgggt aaaaccatcg
     7201 ccattgctat caacttattt aatccgcaaa aaattgttat tgccggtgaa atcaccgaag
     7261 ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaggcg ctgaaggcgt
     7321 ttcgcactaa tctgccggtg gtacgttctg agctggatca ccgctcggca atcggcgctt
     7381 ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg ctggaaaatt
     7441 aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca tgaccattaa
     7501 aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg taccgggtgc
     7561 agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc tgaccaacta
     7621 tccttcgcag accgggcaag atctggcgaa ccgctttgcc actgccggtg tcgatgtgcc
     7681 ggatagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc gccaggaagg
     7741 caagaaagca tatgtggtgg gcgaaggcgc actgattcat gaactgtaca aagccggttt
     7801 cactattacc gatgtgaacc ctgatttcgt gattgttggc gaaacgcgtt cctacaactg
     7861 ggacatgatg cataaagcgg cctatttcgt cgctaacggt gcacgcttta tcgccaccaa
     7921 cccggacacc cacgggcgcg gtttttatcc cgcttgtggc gcgttgtgtg cagggattga
     7981 gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc agcccgtgga tcatccgcgc
     8041 agcattaaac aaaatgcagg cgcattcgga agaaacggtg attgtcggcg ataacttgcg
     8101 caccgacatt ctggcgggct tccaggcagg tctggaaacg attctggtgc tttctggtgt
     8161 ttcatcgctc gacgatatcg acagtatgcc gttccgccca agctggattt acccgtcagt
     8221 cgctgaaatc gacgttatct gaataccacc cacgtctgcg gacgtggttg gctctgatga
     8281 caaacgc
//
LOCUS       DAZFAH010000189         8282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFAH010000189 DAZFAH010000000
VERSION     DAZFAH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.191"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..1908
                     /locus_tag="ACHJWB_003977"
     CDS             130..1908
                     /locus_tag="ACHJWB_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4315525.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNMQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1948..2427)
                     /gene="xdhC"
                     /locus_tag="ACHJWB_003978"
     CDS             complement(1948..2427)
                     /gene="xdhC"
                     /locus_tag="ACHJWB_003978"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4315526.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2424..3302)
                     /gene="xdhB"
                     /locus_tag="ACHJWB_003979"
     CDS             complement(2424..3302)
                     /gene="xdhB"
                     /locus_tag="ACHJWB_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4315527.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3313..5610)
                     /gene="xdhA"
                     /locus_tag="ACHJWB_003980"
     CDS             complement(3313..5610)
                     /gene="xdhA"
                     /locus_tag="ACHJWB_003980"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4315528.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMDDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6025..6780
                     /gene="actS"
                     /locus_tag="ACHJWB_003981"
     CDS             6025..6780
                     /gene="actS"
                     /locus_tag="ACHJWB_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4315529.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6859..6932
                     /locus_tag="ACHJWB_003982"
     tRNA            6859..6932
                     /locus_tag="ACHJWB_003982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6891..6893,aa:Gly,seq:ccc)
     gene            7154..>8282
                     /locus_tag="ACHJWB_003983"
     CDS             7154..>8282
                     /locus_tag="ACHJWB_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO4315530.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKIN"
BASE COUNT         2161 a         1936 c         2064 g         2121 t
ORIGIN      
        1 ttcattagaa aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat
       61 atcatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
      121 ggtaaagcta tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt
      181 cagcgttttg ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa
      241 aacttgtgtc gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc
      301 ggcaactcac gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
      361 tctcgcttcg atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
      421 tttctgggta cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
      481 gttacccacg agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt
      541 cgccatatat ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac
      601 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat
      661 gatgaaagtc gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa
      721 aataatatta ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact
      781 catggacata tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa
      841 ttgcataaca tgcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt
      901 ttttctgtag caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt
      961 gttatgcggc aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg
     1021 gtggttggtg aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc
     1081 ggaagacgga ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg
     1141 gaaagcgaac tgttcggtta tgttaaaggc gcatttactg gcgcttctgc caacggtaaa
     1201 acagggttga ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg
     1261 ccattaatgt tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt
     1321 ggtgccagta gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg
     1381 gcccagttca ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc
     1441 ccgataactc tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac
     1501 tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc
     1561 gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg
     1621 gaatatctgg ttaacgtagt tccttcaggt gaagttatcg acagcacgct attgccgcca
     1681 aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac
     1741 ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc
     1801 gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt
     1861 gctacgctct atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc
     1921 cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt
     1981 tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc
     2041 cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag
     2101 gccaggcgta caaaacccgc actgcactgc gccggatttt gcataagcct gctgaacatg
     2161 agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcttttc cttcagccca
     2221 ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc
     2281 acattcaccc acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc
     2341 cgagagcggc gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc
     2401 gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg
     2461 ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc
     2521 catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc
     2581 aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc
     2641 gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatctag tcggcaatgt
     2701 gcggcgcagc caatcgttga aatatccatc gcgtcgcgca tggcatattt aaaatgcgcg
     2761 ctgcccgcgt gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc
     2821 tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg
     2881 cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg
     2941 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
     3001 gcaatggacg tggccgcagc acataacgcc gggagatgac gttgagttat aggatcttct
     3061 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
     3121 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
     3181 tggtggagct gaatcagtac gtcagtgcca ccggcgagca gtttggcctg cgggttgtca
     3241 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
     3301 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
     3361 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
     3421 ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
     3481 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
     3541 atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa
     3601 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg
     3661 attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
     3721 cgcaatatcg accgtcagat caacaaaggt acagccaaac gccggtgggt tagtggtggt
     3781 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
     3841 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
     3901 ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
     3961 tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
     4021 tgaggcaaat gcgccgggat cgaacggcgt aacgtcggta tcttgagttg aaataacgcg
     4081 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
     4141 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
     4201 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtgt agctaaaaca
     4261 ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt
     4321 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat
     4381 acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc
     4441 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
     4501 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
     4561 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcccccccgc
     4621 agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact
     4681 ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg
     4741 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
     4801 gccaagcttg ctggtcagga atgccgccat tggctcttcc agtacatcct gtttattacc
     4861 aaaaccgcca ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc
     4921 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg
     4981 cgagtcatca tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
     5041 gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
     5101 acccgtcgac atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc
     5161 ttctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac
     5221 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
     5281 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
     5341 gtcaagtgtc caggcatgcc cagcagtagc aaatggaata tcaggcacat cttcccaggt
     5401 aaaaatcgcc agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata
     5461 accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca taacataatc
     5521 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc
     5581 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
     5641 ttatggcaaa gatttagtct aagcaagcaa gatttacgcc agaaacgacg ataagcgtta
     5701 ggggaggaag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct
     5761 gaatagggac agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaatcagg
     5821 attattacgc atcaggatgg gaaatagggt aaaaagtcag gaacttcgat acctctacag
     5881 cgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
     5941 cacgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg atatggcgtc
     6001 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
     6061 atcgtgatgt tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc
     6121 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
     6181 atttcgcgca ccacgggaac cagcgtaaaa gagctggcgc gactgaacgg catctccccc
     6241 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt
     6301 acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg
     6361 gtaccgaaat cttcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg
     6421 aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct
     6481 ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag
     6541 ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac
     6601 gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc
     6661 gccactatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt
     6721 gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga
     6781 tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa
     6841 tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct
     6901 atacgagggt tcgattccct tcgcccgctc caatttatct tcacctgatc aatctgccat
     6961 ttttgctgta tctgattttc tatattatct taattcaatc gcttcagact tttcgcaatc
     7021 gccgtaatac ttccccattt tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg
     7081 taacaaccca ggtaaaacac cttattttca agttgttaaa taagtggtta tctacgcttt
     7141 taacaaacga ttgatggaat attttatgaa agggaaatct gcattaactc ttctgctcgc
     7201 tggtatattt tcgtgcggca catgccaggc gacaggggca gaagtgacga gtgaatctgt
     7261 atttaacatt cttaattcca cgggggcagc aacagataaa agttatttat cgttaaatcc
     7321 agataagtat cctaattatc gtcttttgat tcactcagcc aaattacaaa atgaaataaa
     7381 gagccactat accaaagacg aaattcaagg attgctaaca ttaacagaaa acacacgaaa
     7441 gttaacgttg acggagaaac cctggggaac gtttattctt gcttctactt tcgaagatga
     7501 caaaacagcg gcagaaaccc actatgatgc tgtctggctg agggatagtt tatggggcta
     7561 tatggcatta gtatcagatc agggaaatag tgtagccgca aaaaaggttc tgctcaccct
     7621 gtgggattat atgtctacgc ccgaccagat taagcggatg caggacgtca ttagcaaccc
     7681 caaacgactg gatggagttc ccgggcaaat gaatgttgtg catatccgat ttgacagcaa
     7741 ctcccccgtg atggcagatg tgcaggaaga aggcaagccg cagctgtgga accataaaca
     7801 aaatgatgcg ttaggtcttt atcttgatct ccttatacaa gcaatcgaca ctgatacaat
     7861 taacgctgaa gattggcaaa aaggtgaccg attaaaatcg gttgcgctgc tgattgctta
     7921 tctggataaa gccaatttct atgttatgga agattccgga gcatgggaag aagatgcccg
     7981 attaaacaca tcttccgttg cactggtgac ctctggactg gaacgtttgt cgaacttact
     8041 ctcgaagaaa gactctgttt ttgtctctga tttactacgc gaagctaaag ccaatgaact
     8101 ggatgaacct ctttcaacaa cgaggttgaa ccacctcata gataaaggtt atgaaagaat
     8161 aacgttacag cttgatttag ggggagaatc cccaggttat ctggaaaaag ataaacatta
     8221 cagagaagcc gatgcggctt tacttaatgt tatttatcct gcaaatcttg caaagataaa
     8281 ca
//
LOCUS       DAZFAH010000190         8142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFAH010000190 DAZFAH010000000
VERSION     DAZFAH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.192"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1279
                     /gene="espL4"
                     /locus_tag="ACHJWB_003984"
     CDS             <1..1279
                     /gene="espL4"
                     /locus_tag="ACHJWB_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO4315531.1"
                     /translation="WSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATMNLL
                     MAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSL
                     SETGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNVLPKLAATHHLD
                     NEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTCHLTKEQVLDLLKA
                     KDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLARDTGLF
                     MAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNV
                     VETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNK
                     MAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1248..2984)
                     /gene="aceK"
                     /locus_tag="ACHJWB_003985"
     CDS             complement(1248..2984)
                     /gene="aceK"
                     /locus_tag="ACHJWB_003985"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4315532.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3265..4569)
                     /gene="aceA"
                     /locus_tag="ACHJWB_003986"
     CDS             complement(3265..4569)
                     /gene="aceA"
                     /locus_tag="ACHJWB_003986"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4315533.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4599..6200)
                     /gene="aceB"
                     /locus_tag="ACHJWB_003987"
     CDS             complement(4599..6200)
                     /gene="aceB"
                     /locus_tag="ACHJWB_003987"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4315534.1"
                     /translation="MTEQATTTDELAFIRPYGEHEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6469..7398)
                     /gene="metA"
                     /locus_tag="ACHJWB_003988"
     CDS             complement(6469..7398)
                     /gene="metA"
                     /locus_tag="ACHJWB_003988"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4315535.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7555..7998
                     /locus_tag="ACHJWB_003989"
     CDS             7555..7998
                     /locus_tag="ACHJWB_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4315536.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2030 a         2028 c         2045 g         2039 t
ORIGIN      
        1 atggtcaaat gatcatgtta ttaaccaatc tgttgcaata attccagcac ttccgaaaga
       61 acagctattg atgttaaaag gatctgtgga tgaaataacc cctccattat cacctgcaac
      121 gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcagct
      181 ccagaaaatg ccagaactac atagaacaga aatgttgact gcctataata gtattaacct
      241 ccccggttta tatttggcta taaattatgg caatgcggat atcgttgaga ctattttcaa
      301 ttcattgtca gaaacaggat atgaagggtt actctcgaaa aaaaatctca tgcatattct
      361 ggaggcaaaa gataaaaatg gtttttctgg attattttta gcgatatcac gtaaggataa
      421 aaatgttgta acctcgattc tgaacgtctt acctaaactg gccgcaacac atcatttaga
      481 taatgaacag gtgtataaat tcctgagtgc caaaaataga acgtccagcc atgttttata
      541 ccatgttatg gcgaatggtg atgccgacat gctgaaaatt gttttggacg cattaccttt
      601 gttaattcgc acatgtcatt tgactaaaga acaggtactc gatctcctga aggcaaagga
      661 tttttatggt tgcccaggac tatacctagc gatgcaaaat ggacatagcg atatcgtgaa
      721 agttattctt gaagcattgc ccagcctggc ccaggaaatt aacatttcag cttccgatat
      781 tgtagatctt ctgaccgcta aaagtcttgc gcgcgacacg ggtttgttta tggccatgca
      841 gcgcggacat atgaacgtta ttaatactat ttttaacgca ttacccactc tgtttaatac
      901 gtttaaattc gataaaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
      961 tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct
     1021 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
     1081 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
     1141 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
     1201 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
     1261 tccccatacc gtacgctaaa tctttgcctg cgccgatatg cataaacatc ttccacatgc
     1321 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
     1381 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
     1441 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
     1501 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
     1561 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
     1621 gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
     1681 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctca
     1741 atataaagat ggcgaatcac aatttgttcg ccgagatcgg tgattttttc cgctgcttcc
     1801 tggagcagta attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca
     1861 aactcctggg tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgataacag
     1921 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatcacttta
     1981 aatacccggt caaagcccgg cagcgtgaac accaacatca ccatgccacg aatacccggc
     2041 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
     2101 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcggtggt tttacctggc
     2161 agaatttccc gtagccactc gaccagcgct gcgggcagcg gcgcataaac cataaaataa
     2221 gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata
     2281 aataactcgc cgtcgtccgt ctggtggatc ggcagcaaaa atggcaatgt gccggaaggt
     2341 gtgatcagtt tgcctaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc
     2401 tgtaaatgac tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg
     2461 taatgaatgt cacggctttt attctgccag cgcaggcgca gcggtaggtc gctgataacg
     2521 cgcatcagta gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga
     2581 atggtacgaa agcggcgctc tggctgagag ctaaagataa aaagccgctc gggagtaagc
     2641 gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag
     2701 cgcgggtaat ccggcaacag ccgggtgtaa tgctctttaa cacgtagtaa aaatgccgcg
     2761 tccgtacttt ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga
     2821 tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg
     2881 aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct
     2941 tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag
     3001 gagcagagaa ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt
     3061 gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
     3121 gcgtaaatgc cttatccggc ctacgttcgg caacggctgt aggcatgata agacgcgcaa
     3181 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtgaacgcct tatccggcct
     3241 acagtcagca acggttgttg ttgcttagaa ctgcgattct tcagtggagc cggtcagcgc
     3301 ggtgactgaa gacgtgccgc cctgaataat agtcgtcact ttatcgaagt aacctgtacc
     3361 cacttcctgc tggtgagata cgaaggtata gccatctttc gcggcggcaa attccggctg
     3421 ctgcactttc tcaacgtagt gcttcatacc ctcgccctgg gcataggcgt ttgccaggtc
     3481 aaacatgttg aaccacatgc tgtggatacc tgctagggtg atgaactgga acttgtagcc
     3541 catatccgac agctgctgct ggaagctggc aatagttttg tcgtcgaggt ttttctgcca
     3601 gttgaacgac ggcgagcagt tataagccag cagtttgccc ggatatttcg cgtggatagc
     3661 ttgtgcaaag cgacgcgcca gttccagatc cggcgtggag gtttcacacc agaccaggtc
     3721 agcatatggc gcatacgcca ggccacggct gatcgcttgc tcaatgcccg catgagtacg
     3781 gaagaagcct tcactggtac gctcgccagt aataaattca ctgtcatacg ggtcgcaatc
     3841 ggaggtaatc agatccgccg catcggcatc ggtacgagca accagcaggg ttggaacgcc
     3901 cgtcacgtca gctgcaagac gcgccgcgac cagtttctga atagcttcct gagttggcac
     3961 caaaactttg ccgcccatgt gaccgcattt cttcactgac gccagctgat cttcgaagtg
     4021 aactgccgct gcaccggctt caatcatcgc tttcatcagt tcaaaggcat tcaggacacc
     4081 gccaaaaccg gcttccgcat cggcaacgat cggcaggaag taatcgacat agcgcggatc
     4141 gcccggctca atgcctgcgg accattggat ctgatcggca cgacggaagg tgttgttgat
     4201 ccgctccacc acagctggca ccgagtttgc cggatagagc gactgatccg gatacatgct
     4261 ggccgccagg ttagcgtccg ccgctacctg ccatcccgac agatagactg cttcaatacc
     4321 cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc
     4381 ttttttcgac tcgccatgca gcagacgcca cattttcgcc gcgcccagtt gcgccagggt
     4441 gcattcagga ttgactgaac cgcgtaattt caccacatct tccgcgctgt atgggcgagt
     4501 aatgccttcc caacgcggtt gagtccactc tttctgtaat tcttcaattt gttgtgtacg
     4561 ggttttcatg tgcagatgct ccatagttat gtggtggttt acgctaacag gcggtagcct
     4621 ggcagggtca ggaaatcaat taactcatcg gaagtggtga tctgttccat caagcgtgcg
     4681 gcatcgtcaa aacgcccctg ggagaaacgt tcttcgccca gttcgctggc aatgactttc
     4741 atctcttcac ccagcatctg gcggaacaag gctttggtca ccggtttgcc attgctcaac
     4801 gttttttgat gatggatcca ctgccagatt gaggtacggg aaatttcagc cgtcgccgca
     4861 tcttccatca ggccataaat cggcacgcag ccgttgccgg agatccatgc ttcgatgtac
     4921 tgcactgcca cgcgaatgtt ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt
     4981 gccagcagct gatcggcagt aatcggcgcg tcttgttcgc gcatcacttc aagctgattt
     5041 ttacgggagc cgagaatgtc gttgaatacc gccattgccg tatccgcaag gcctgggtga
     5101 gcaatccatg tgccatcgtg accgttattg gcttccagcg ctttatccgc ttttactttg
     5161 ttgagcacct ggttattgcg ctcttcatct ttgctcggaa taaacgctgc catgccgccc
     5221 atcgcaaaag caccgcgttt atggcaggtt ttaatcaaca ggcgtgagta agcattcagg
     5281 aatggtttat ccatcgtcac tgcctgtctg tctggcagga cgcgatcggg atagtttttc
     5341 aacgttttga tatagctgaa gatgtaatcc caacgaccgc agttcagacc aacaatatgg
     5401 tcacgcagcg cgtgaaggat ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc
     5461 aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata gctgaagact
     5521 tcgctccacc aggccgcttc ctgccaggac tgggttttcg gcagatagaa atagggaccg
     5581 ctgccctttg ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa atcaaacagg
     5641 ctaccgggga ttgcctcacc acgccaggtg acatgttttt ccggcaagtg cagaccgcgt
     5701 acccgacaaa tcaaaaccgc tggattgggc ttgagctgat aaattttgcc tgcttcattg
     5761 gtatagctga tggtgccgtt aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg
     5821 ttccagtctg gtgccagtga atcttcgaaa tcggccataa agactttcac attggcgttc
     5881 agtgcgttga tcaccatctt gcgctcaacc gggccagtta tctcgactcg acgatcttgt
     5941 aagtccgcgg gaataccacg aattttccag tccgcatcgc gaatggaagc tgtttccgaa
     6001 ataaaatcag gcaaagttcc gttatcaata tcttgctgct gctgaatgcg cgctgccaga
     6061 agtttattgc gttgtggcgt aaaatgcgtc accagctcag tcagaaattc taccgcttcg
     6121 gcagtaagaa tttgcttctc atgctcgcca tacggcctta tgaaagccag ttcatcggtt
     6181 gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg atccgaaaac ttccccactg
     6241 tgaacgaagg atcgttgtgc gcttttcgtt cagcacaact aagactactc atttaaaatg
     6301 caaaatcaaa aacaatttcc attttaatta aaaatacttg ataacttatt gataacaata
     6361 tgaataaaat ttattcacct cctgaggtgc gtttcggaaa taaaaaaggc acccgaaggt
     6421 gcctgaggta aggtgctgaa tcgcttaacg atcgactatc acagaagatt aatccagcgt
     6481 tggattcatg tgccgtagat cgtatggcgt gatctgatag acgtaatagt tgagccagtt
     6541 ggtaaacagc aaattaccgt gactacgcca gctcgctcgc ggtgtatttt gcggatcatt
     6601 gtgcgggaaa tagttatacg gtacatccgg gtctagtccg gcttccacat cgcggaaaaa
     6661 ttcctgcgcc agcgtttgcg catcatattc gggatggccc gtcacaaagg caatgcgctt
     6721 atctttgctg gcaaacagat atgcatcccc ttcttccgtc tctgccagaa tttccagatc
     6781 ggtgtaatca cgaatcaacg ctgccggaaa gtcagcatag cgcgaatgtg gtgccaggaa
     6841 tgaatcatca aagccacgcg tcagaagcgc atgaggatgg agaatatgat gctcgtaaac
     6901 gccagagagt ttgtcggtgc gagtttgctt aggaatgccg tagaggatat tgagcgcggc
     6961 ctgtaccgcc cagcagacaa acagcgtcga ggtgacgtga tctttcgacc actccagcac
     7021 ctgtttgatc tgcggccagt aagcgacatc attaaactcc accaggccca gcggcgcgcc
     7081 ggtaactatc agaccgtcaa agttttgttc ctgaatatct tcaaagttac agtagaagtt
     7141 gttcagatgc tctgcgggcg tgttgcgcga ttcacgggaa tcgatgcgca acagctgaat
     7201 atcgacctgt aagggcgagt ttgaaagcag gcgcagaaac tgattttcag tttcgatctt
     7261 cttcggcatc aggttaagga taagcacctt tagcggacga atttcctgac cagacgcgcg
     7321 agaagttgtc atcacaaaga cattttcttc acgcaagaaa ttgacggcgg gtagctcgtc
     7381 cggcacacga atcggcataa cctgattacc tcactacata cgcttatacg tttagacatc
     7441 cagatagctg aagataacca gaaaatttgc caatgtcgag cctgcatgtt gaaggtgaga
     7501 aagtttcaag gaaaaggcta cgttagaata taagaatgac gaaaaggaga gaaaatggtt
     7561 attagtattc gccgctcaca gcatgaggaa ggggaggaac tcgttgcgat ttggtgtcga
     7621 tctgtcgatg ccacccacga ttttttatca gcagagtatc gggccgagct ggaagagctg
     7681 gttcgttcct ttctgccgga agcaccgttg tgggtcgcgg ttaatgaacg ggatcagccg
     7741 gttgggttta tgttgttaag tgggcagcat atggatgcgc tgtttatcga tcctgatgtg
     7801 cgcggctgcg gcgtgggtcg gatgctggtg gagcatgcgc tctcgatggc accggaactg
     7861 acaaccaacg ttaatgagca aaatgagcag gcggttgggt tctataagaa ggtgggtttt
     7921 aaggttacgg gacgctctga ggtggacgat ttggggaaac cgtatccgtt gctgaatctg
     7981 gcgtatgtgg gggaataagc cgcccctgcc gtgcctgccc aggctggaga agacgcgcca
     8041 gcgtcgcacc cggcattttt ttcaccaacg caaaaaggcc atcctgacgg atggcctttc
     8101 gcttaatttg atgcctggca gttccctact ctcgcatggg ga
//
LOCUS       DAZFAH010000191         8135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFAH010000191 DAZFAH010000000
VERSION     DAZFAH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.193"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1967
                     /gene="acnA"
                     /locus_tag="ACHJWB_003990"
     CDS             <1..1967
                     /gene="acnA"
                     /locus_tag="ACHJWB_003990"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4315537.1"
                     /translation="LGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     ILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASE
                     PIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHJWB_003991"
     CDS             complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHJWB_003991"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4315538.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHJWB_003992"
     CDS             2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHJWB_003992"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4315539.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3704..4012
                     /gene="lapA"
                     /locus_tag="ACHJWB_003993"
     CDS             3704..4012
                     /gene="lapA"
                     /locus_tag="ACHJWB_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4315540.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4019..5188
                     /gene="lapB"
                     /locus_tag="ACHJWB_003994"
     CDS             4019..5188
                     /gene="lapB"
                     /locus_tag="ACHJWB_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4315541.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5381..6118
                     /gene="pyrF"
                     /locus_tag="ACHJWB_003995"
     CDS             5381..6118
                     /gene="pyrF"
                     /locus_tag="ACHJWB_003995"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4315542.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDMKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGNDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6118..6444
                     /gene="yciH"
                     /locus_tag="ACHJWB_003996"
     CDS             6118..6444
                     /gene="yciH"
                     /locus_tag="ACHJWB_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4315543.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6570..6788)
                     /gene="osmB"
                     /locus_tag="ACHJWB_003997"
     CDS             complement(6570..6788)
                     /gene="osmB"
                     /locus_tag="ACHJWB_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4315544.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7057..7806)
                     /gene="yciT"
                     /locus_tag="ACHJWB_003998"
     CDS             complement(7057..7806)
                     /gene="yciT"
                     /locus_tag="ACHJWB_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4315545.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(7896..8069)
                     /gene="yciZ"
                     /locus_tag="ACHJWB_003999"
     CDS             complement(7896..8069)
                     /gene="yciZ"
                     /locus_tag="ACHJWB_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4315546.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         2041 a         1901 c         2130 g         2063 t
ORIGIN      
        1 tgttaggcca gccggtttcc atgcttatcc cggacgtagt gggctttaaa cttaccggta
       61 aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca
      121 aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgc
      181 tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct
      241 tcccaatcga tgctgtaacc ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg
      301 ttgagttggt cgaaaaatat gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac
      361 cgatttttac cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc
      421 caaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta
      481 acgaactgga agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg
      541 gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca
      601 acacctctaa cccaagtgtg ctgatggctg caggcttgct ggcgaaaaaa gccgtaattc
      661 ttggcctcaa gcggcaaccg tgggtcaaag cgtcgctggc accaggctcg aaagttgttt
      721 ctgactatct ggcaaaagcg aaactgacac cgtttctcga cgaactgggg tttaaccttg
      781 tgggatacgg ttgtactacc tgtattggta actctgggcc gctgcccgat cctatcgaaa
      841 cggcaatcaa aaaaggcgat ttaaccgtcg gtgcagtgct gtccggcaac cgtaactttg
      901 aaggccgtat ccatccgctg gttaaaacca actggctggc ctcgccgccg ctggtggttg
      961 cctatgcgct ggcgggaaat atgaatatca acctggcttc tgagcctatt ggtcatgatc
     1021 gtaaaggcga tccggtttat ctgaaagata tctggccatc ggcacaagaa attgcccgtg
     1081 cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca
     1141 cagcagagtg gaaggaaatt aacgtcacac gatccgatac ctacggttgg caggaggact
     1201 caacctatat tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcaccagtgg
     1261 aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata
     1321 tctctccggc gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggtcggg
     1381 gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga
     1441 tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg
     1501 ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct
     1561 ataagcagga acaaacgccg ctggcggtga ttgccgggaa agagtatggt tcaggctcaa
     1621 gccgtgactg ggcggcaaaa ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat
     1681 cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc
     1741 cgcaaggcgt aacgcgtaaa acgttaggac taaccgggga agagaagatt gatattggcg
     1801 atctgcaaaa tctacaaccc ggtgcgacgg ttccggtgac gcttacgcgt gcggatggta
     1861 gccaggaggt cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc
     1921 agaacgacgg cattttgcat tatgtcattc gtaatatgct gaagtaacaa gtatttgctt
     1981 gccggttatt ttgcttccgg caagcaaact aattacacaa tgcaagaggg ttatttgttc
     2041 agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga
     2101 cctacaatca atggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact
     2161 tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca
     2221 gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg
     2281 gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta
     2341 cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat
     2401 gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga
     2461 cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc
     2521 gcgacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat
     2581 ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc
     2641 tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc
     2701 gatcgttcac ggaatggcac aaatctgtca atttttcctg gaactggcgt tttcagttat
     2761 gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg
     2821 ggagctgcac tattgcttgt catgccagta gccgtatgga tttctggctg gcgttggcaa
     2881 cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag
     2941 ccctggggcg tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt
     3001 cgcattaagg ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgtgggacaa
     3061 ggcgttaaat cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggctg
     3121 gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga
     3181 aatttagtga aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacac
     3241 tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg
     3301 gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg
     3361 ctggtctggg caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc
     3421 gatctggtag tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg
     3481 caacgaattt gtgggccatt aacaccacct gcggaagaaa atcgcgaaat agcgcaacga
     3541 gaacaagaaa gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc
     3601 gcgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag
     3661 ttgaccataa tttattcgct ctaaccacat aacgagaagt aatgtgaaat atttactcat
     3721 tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca
     3781 acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc
     3841 ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg
     3901 agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc
     3961 acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat
     4021 gctggagttg ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg
     4081 cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc
     4141 gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat
     4201 gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc
     4261 gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct
     4321 gacctatgaa cagcgtctgt tggcgattca acaactaggg cgtgattaca tggccgccgg
     4381 gttatacgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat
     4441 tggcgcgctg caacagttgc tacaaatcta ccaggctacc agtgagtggc agaaagcaat
     4501 tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca
     4561 tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac
     4621 cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg
     4681 acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat
     4741 atcccaggac agagaactgg tcagcgaaac gctggaaatg ttgcaaacct gctatcagca
     4801 gttgggtaaa actgccgaat gggcagaatt cctgcaacgc gcggtggaag agaacaccgg
     4861 tgccgatgct gaattgatgc ttgcggatat catcgaagcg cgcgacggta gtgaggctgc
     4921 acagatctat attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt
     4981 aatggattac cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct
     5041 gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg
     5101 ttttaccgca tacaccctct actggcattg tccgtcttgt cgggcctggt caaccattaa
     5161 accgattcgc ggtcttgatg gcctgtaatt tttaaaaaaa tccgacttta gttacaacat
     5221 actagatatt gatgttccat tgtgctccgg caacgacgga gcagaaaaag cctgcctggg
     5281 gagaaatcgc aactgttaat tttttatttc cacgggtaga atgctcgccg tttacctgtt
     5341 tcgcgccact tccggtgccc atcatcaaga aggtctggtc atgacgttaa ctgcttcatc
     5401 ttcttcccgc gctgttacga attctcctgt ggttgttgcc cttgattatc ataatcgtga
     5461 tgccgcgctg gcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa
     5521 agagatgttt acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga
     5581 tatctttctt gacatgaaat tccacgatat ccccaacact gcagcgcacg ctgtcgctgc
     5641 tgcagctgac ttaggcgtgt ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat
     5701 gaccgcagcg cgtgaggcac tggttccgtt tggcaatgat gcaccgcttt tgattgctgt
     5761 gacagtgttg accagcatgg aagctagcga cctggccgat cttggcgtta cactgtcacc
     5821 tgcagattat gcagaacgtc tggcagcact gacgcaaaaa tgtggccttg atggtgtggt
     5881 gtgttctgct caggaagctg tacgtttcaa acaggtattc ggtcaggagt tcaaactggt
     5941 tacgccgggc attcgtccgc aggggagtga agctggtgac cagcgccgca ttatgacgcc
     6001 agaacaggcg ttgtcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc
     6061 ggtagatcca gcgcagacgc tgaaagcgat caacgcctct ttacagcgga gtgcatgatg
     6121 agtgattcca acagccgtct ggtctactca acggaaaccg gacgtattga tgagcccaaa
     6181 gcggcccctg tacgccctaa aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga
     6241 cgtaaaggta agggcgtttg cctgattacc ggtgtcgatc tcgatgatgc cgaactgaca
     6301 aaacttgcag cggaactgaa gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt
     6361 attgaaatcc agggcgataa gcgtgattta ttaaaatcac tacttgaagc gaaagggatg
     6421 aaggtaaaac tcgcaggcgg ttaacataaa aagccacgga tatatccgtg gctttcgaat
     6481 attttactgt gcgtattatt cagttatttc gtactgaaca gaatcaagcg tattataatt
     6541 gcgagtcgat aatcgacatt tatttacgat tatttaccga cctggtgacc aataacacca
     6601 ccgacagctg caccacctaa tgtacccaac gtactgccat cggtcagtac tgcaccgcct
     6661 aatgcccctg cacccgcgcc gattgcggtg ttgcggtccc gtttagacca gttagaacag
     6721 gcactcagag acattgccaa agtaattgcc agaacagccg cggtcatttt tttgctcgtt
     6781 acaaacataa tactctctcc tgaatttatg attcacggaa gtaagctctc tataactata
     6841 atagctaaga ataagcctgg tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata
     6901 tcacgcaggt gataatgact tcctgttata tcgctgataa taattttata tcttaagcgt
     6961 gttaataaca ggtaaatagt cttaattatc aaccaggaat catcttagag cggatgattt
     7021 gccaaactgc aaatcatccg tagagaaggg aaatggttaa atgtcaacga cgtgaacggt
     7081 gagtttactg tgctcaagat gcatcagatc gctggcgcgt attttcgaat cggtaatcac
     7141 gcgattgaat cgctcaacgg gaccgatgga gtatgaatgt acagcaccaa atttcgagct
     7201 gtcagtcagg actatcgctt cgcactcttt ttccagcacg gcattgacca catcggtacg
     7261 catcatatcg cgaccggtga atccagtttc aggttgccag ccatcaatac caataaatgc
     7321 tttgctgaaa tgtacctgct ggatgcactg gcgtgtcaaa gggccaacca tactttcgct
     7381 ttttttctgg tacacgccac cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg
     7441 cgcaatgtag ctgctgactg tgatgatagt gacatttttc ttctgttcgc ccagagtccg
     7501 ggcgagcagg gcattgctgc taccattttc gataaagatg gtttcgcccg gttgaaccag
     7561 agacgcggca aactcggcga gttcacgctt cagcgtatag ttgctcatca tacgggtttc
     7621 gacgtcatca ctatcaagcg aaactgcaaa gccatgtgca cggcggaggt aactcagttt
     7681 ttcgagggtg ttgagatcct ggcgaatggt aacttcagaa actccagtgg cttttgccag
     7741 atcggttacg ctaacctgac cctggtcaat gaccatctgt agaatagttt gttgtcggga
     7801 attcatcgct gtaattctta taacgttata atactaaata aaaaaatact gacgcgccgg
     7861 gcatcctcaa ataggaaaac ctgaaggcac gattgttaaa tctcccacgg tgccttcggg
     7921 atgtctggcg tagattcagc aacctggcgc aagagttctg ctttaagtat ctcgagatta
     7981 tggatcgcag agtggtaatc gcctgccaca aggatatcta acacaatatc aatacgttcg
     8041 gcaacgcgct gggcatcaaa ttcagacatc aaggacatcc ttatagtaaa agtggaaaat
     8101 gaataatgca aaaattgccg ctaaaagaga agtgt
//
LOCUS       DAZFAH010000192         8099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFAH010000192 DAZFAH010000000
VERSION     DAZFAH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.194"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..277)
                     /locus_tag="ACHJWB_004000"
     CDS             complement(44..277)
                     /locus_tag="ACHJWB_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4315547.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            666..1211
                     /locus_tag="ACHJWB_004001"
     CDS             666..1211
                     /locus_tag="ACHJWB_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4315548.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1186..3111
                     /locus_tag="ACHJWB_004002"
     CDS             1186..3111
                     /locus_tag="ACHJWB_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4315549.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDIAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLAEYARALSGEDE"
     gene            3108..3314
                     /locus_tag="ACHJWB_004003"
     CDS             3108..3314
                     /locus_tag="ACHJWB_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4315550.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3311..4912
                     /locus_tag="ACHJWB_004004"
     CDS             3311..4912
                     /locus_tag="ACHJWB_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4315551.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4893..6212
                     /locus_tag="ACHJWB_004005"
     CDS             4893..6212
                     /locus_tag="ACHJWB_004005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4315552.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQACVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6222..6554
                     /locus_tag="ACHJWB_004006"
     CDS             6222..6554
                     /locus_tag="ACHJWB_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4315553.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6610..7635
                     /locus_tag="ACHJWB_004007"
     CDS             6610..7635
                     /locus_tag="ACHJWB_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4315554.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEEPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSATN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7677..8072
                     /locus_tag="ACHJWB_004008"
     CDS             7677..8072
                     /locus_tag="ACHJWB_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4315555.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDGAGQDSSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
BASE COUNT         1847 a         2072 c         2434 g         1746 t
ORIGIN      
        1 tgtgatatga gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctgacct
       61 gttttctgag caattcagta atgtcactct tttctttgtt tgcttcagaa gaaactcttt
      121 tttctgagca cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc
      181 ctttttgctc aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc
      241 gggttgttgt tctgcgggtt ctgttcttcg ttgacatgag gttgccccgt attcagtgtc
      301 gctgatttgt attgtctgaa gttgctttta cgttaatttg atgcagatca attaatacga
      361 tacctgcgtc ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat
      421 gtagatgata attattatca ttttgcgggt cctttccggc gatccgaccg gttacggggc
      481 ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct
      541 tcttcgccgt aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag
      601 gtgctgaaaa cgggcttttt ggcctttgtc gtttcctttc tctgtttttg tccgtggaat
      661 gaacaatgga agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca
      721 ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg
      781 tgctttatga ttctgccgcc gtcataaaat ggtatgccga aagggatgct gaaattgaga
      841 acgaaaagct gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc
      901 cagggactat tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc
      961 tgaaaaatgc cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt
     1021 cgcggatcgc aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc
     1081 gttttccgga actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca
     1141 tgaacaaagc agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac
     1201 agtcaggtta acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg
     1261 gagccacaga ccgccgttga atgggcggat gccaattact atctcccaaa agaatccgca
     1321 taccaggaag ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc
     1381 agcgactaca tccgcgaggt gaatgtggtg aagtctgccc gtgttggtta ttccaaaatg
     1441 ctgctgggtg tttatgccta cttcatagag cataagcagc gcaacacact tatctggttg
     1501 ccgacggatg gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat
     1561 attccgtcgc tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc
     1621 accatgaagc gtttcaccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa
     1681 aactaccgtg aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag
     1741 gatattgaac aggaaggctc tccgacgttc ctgggcgata agcgtattga aggctcggtc
     1801 tggccaaagt ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt
     1861 gcagccagtg aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag
     1921 gagcagtatc ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat
     1981 gacccctcca gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag
     2041 ctggacttta ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc
     2101 attctctggt tttcgtcatc cggtgaagag attgaaccgc ctgacagtgt gacctttcac
     2161 atctggacgg cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaag
     2221 acgaaagggg atacgggaaa acgtaaaacc ttcgtgaaca ccacgctcgg tgagacgtgg
     2281 gaagcgaaaa tcggcgaacg tccggatgct gaagtgatgg cagagcggaa agagtattat
     2341 tcagcgcccg ttcctgatcg tgtggcttac ctgaccgccg gtatcgactc ccagctggac
     2401 cgctacgaaa tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgaccgg
     2461 cagattatta tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc
     2521 aataaaacct atacccgccg gaatggtgca gaaatgtcgg tatcccgtat ctgctgggat
     2581 actggcggga ttgacccgac cattgtgtat gaacgctcga aaaaacatgg gctgttccgg
     2641 gtgatcccca ttaaaggggc atccgtctac ggaaagccgg tggccagcat gccacgtaag
     2701 cgaaacaaaa acggggttta ccttaccgaa atcggtacgg atatcgcgaa agagcagatt
     2761 tataaccgct tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc
     2821 ccgaataacc cggatatttt tgatcttacc gaagcgcagc aactgactgc tgaagagcag
     2881 gtcgaaaaat gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat
     2941 gaggcgctcg actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg
     3001 cagctggatc tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac
     3061 aagaaaacac tggcagaata cgcccgtgcc ttatccggag aggatgaatg acgcgacagg
     3121 aagaacttgc cgctgcccgt gcggcactgc atgacctgat gacaggaaaa cgggtggcaa
     3181 cggtacagaa agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa
     3241 aatacattgc ggagctggaa gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag
     3301 gattttatgt atgaaaacgt ccaccattcc cacccttctg gggccggacg gcatgacatc
     3361 gctgcgtgaa tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc
     3421 gtggaaccca ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa
     3481 tgcccgcgca gacgatctgg tacgcaataa cggctatgcc gccaacgcca tccagttgca
     3541 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     3601 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     3661 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     3721 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     3781 ggataccagt ccgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     3841 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     3901 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     3961 gaaatggaca tggatacccc gtgaattacc cggcgggcgc gcctcgttca ttcacgtttt
     4021 tgaacccgtg gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca
     4081 gatgaagatg ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat
     4141 gtatgccgcc accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg
     4201 cgcgaacagt caggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta
     4261 ttacgccgca gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga
     4321 ctcactgaac ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc
     4381 actgttgcgg tatatcgctg ccgggctggg tgtctcgtat gagcagcttt cccggaatta
     4441 cgcccagatg agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat
     4501 ggggcggcga aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga
     4561 agaggccatc gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga
     4621 agcccgcagt gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg
     4681 tctgaaagaa gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa
     4741 agagtgcgcg aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac
     4801 gatggagcgc cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc
     4861 cggactgcga caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc
     4921 atattgccag catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt
     4981 tcttttgtgc gcttgcaggc cagcttggga tcagccgcct gacggatgca gtatccggcg
     5041 acagcctgac tgccggagag gcactcgcga cgctggcgtt atccggtgat gatgacggac
     5101 cacgacaggc ccgcagttat caggtcatga acggcatcgc cgtgctgccg gtgtccggta
     5161 cgctggtcag ccggacgcgg gcgctgcagc cgtattcggg aatgaccggt tacaacggca
     5221 ttatcgcccg tctgcaacag gctgccagcg atccgatggt ggacggcatt ctgctggata
     5281 tggacacacc gggcgggatg gtggcgggag catttgactg tgctgacatc atcgcccgtg
     5341 tgcgagacat aaaaccggta tgggcgctgg ccaacgacat gaactgcagt gcaggtcagc
     5401 tgcttgccag cgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg
     5461 gcgtcatgat ggctcacagt aattacggtg ctgcgctgga gaaacagggc gtggaaatca
     5521 cgctgattta cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg
     5581 atgtccggga aacactgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg
     5641 tgtcggcata taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca
     5701 gcggtcagga ggccattgat gccggactgg ctgatgaact tgtgaacagt accgatgcga
     5761 tcaccgtcat gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga
     5821 ccaaagagac tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg
     5881 tggtgcaagc gacggagggc gagaacgcca gcgctgcgca gccggacgtg aacgcgcaga
     5941 tcaccgcagc ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg
     6001 ctcacggacg cgaagaacag gcatgcgtgc tggccgaaac ccccggtatg accgtggaaa
     6061 cggcccgccg tattctggcc gcagcaccac agagtgcaca ggcgcgcagt gacactgcgc
     6121 tggatcgtct gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg
     6181 ccgttaacga tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct
     6241 ttacccatta ccagccgctg ggcaacagtg acccggctca taccgcaacc gcgcccggcg
     6301 gattgagtgc gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc
     6361 tggttgcgtg ggatggcacc accgacggtg ctgccgttgg cattcttgca gttgctgctg
     6421 accagaccag caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc
     6481 tctggccgga ggctgccagc gacgagacga aaaaacggac cgcgtttgcc ggaacggcaa
     6541 tcagcatcgt ttaacctgac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg
     6601 gattttttta tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt
     6661 aagtttgatc cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag
     6721 aaagtctatc tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt
     6781 gtttccggtg aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat
     6841 gtcaaaccca agcatgaagt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagaa
     6901 ccgcagaatc tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt
     6961 gacgaagagc tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag
     7021 ggcaaataca ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt
     7081 gcgacgaaca acatcacgca gtccggcggc acggagtgga gcaagcgtga caagtccacg
     7141 tatgacccga ccgacgatat cgaagcctac gcgctgaacg ccagcggcgt ggtgaatatc
     7201 atcgtgtttg acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt cagggagaag
     7261 ctggataccc gtcgcggctc tcattccgaa ctggagacag cggtaaaaga cctgggcaaa
     7321 gcggtgtctt ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac
     7381 gtggaaaacg gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct gggtaacact
     7441 catgcacgcg gtctgcgcac ctatggctgt attcaggatg cggatgcatt gagtgagggt
     7501 attaatgcgt ctccccgtta tccgaaaaac tggaagacat ccggcgatcc ggcgcgagag
     7561 ttcaccatga ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc
     7621 gttcaactgg cgtaatcatg gcccttaggg gccattttct ctctgtggag gaatccatga
     7681 cgaaagatga actgattgcc cgtctccggt cgctgggtga gcaactgaac cgtgatgtca
     7741 gcctgacggg gacgaaagaa gaactggcgc tccgtgtggc agagctggaa gaggagcttg
     7801 atgacacgga tgacggagcc ggtcaggact cgtctgtcag cccggaaaat gcgctgaccg
     7861 gacatgaaaa tgaggtggta tcagcacaga cggataccgt gactgatacg gctgctctgg
     7921 tcacggttgt ggcactggtg acgctgcata ccgatgcact tcacgccacg cgggatgaac
     7981 ctgtggcatt tgtgctgccg ggaacggcgt ttcgtgtctc tgccggtgtg gcagccgaaa
     8041 tgacagagcg cggcctggcc agaatgcaat aacgggaggc gctgtggctg atttcgata
//
LOCUS       DAZFAH010000193         8050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFAH010000193 DAZFAH010000000
VERSION     DAZFAH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.195"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2756
                     /gene="evgS"
                     /locus_tag="ACHJWB_004009"
     CDS             <1..2756
                     /gene="evgS"
                     /locus_tag="ACHJWB_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4315556.1"
                     /translation="LDTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTD
                     ENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDREN
                     NVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVD
                     NASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEP
                     ELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSL
                     LWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYF
                     TADYYKNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHW
                     HTLCNLPASDNAVYICGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRT
                     PISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQP
                     QWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNAL
                     KFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTG
                     SGLGLMICKELIKNMQGNLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPE
                     KLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNM
                     DGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQL
                     HQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRT
                     FHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEI
                     AVF"
     gene            complement(2827..3972)
                     /gene="yfdE"
                     /locus_tag="ACHJWB_004010"
     CDS             complement(2827..3972)
                     /gene="yfdE"
                     /locus_tag="ACHJWB_004010"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO4315557.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHNKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4046..4990)
                     /gene="yfdV"
                     /locus_tag="ACHJWB_004011"
     CDS             complement(4046..4990)
                     /gene="yfdV"
                     /locus_tag="ACHJWB_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4315558.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLNPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     MAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5060..6754)
                     /gene="oxc"
                     /locus_tag="ACHJWB_004012"
     CDS             complement(5060..6754)
                     /gene="oxc"
                     /locus_tag="ACHJWB_004012"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4315559.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVIL
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6808..>8050)
                     /gene="frc"
                     /locus_tag="ACHJWB_004013"
     CDS             complement(6808..>8050)
                     /gene="frc"
                     /locus_tag="ACHJWB_004013"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4315560.1"
                     /translation="PLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRH
                     QLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHM
                     GFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVS
                     AAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKL
                     GYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWEN
                     TCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAP
                     VLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAA
                     VLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2351 a         1876 c         1688 g         2135 t
ORIGIN      
        1 ggcttgatac aggaaacctg gcctttctga acaaaccatt agaactcact gaacatgaaa
       61 aacagtggat taagcagcat cccaatttaa aggtgctgga aaatccttac tcgcccccct
      121 attctatgac ggatgaaaat ggctcggttc ggggcgttat gggggacatt cttaatatta
      181 ttaccttgca aacaggttta aatttttctc cgatcaccgt ttcacacaat atccatgctg
      241 gaacacagct tagccccgga ggatgggata taatacctgg cgctatttat agtgaagatc
      301 gagaaaataa tgttttattt gctgaagcct tcataacaac gccttacgtt tttgtcatgc
      361 aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc attccatatt
      421 attatgagct gcattcgcaa ttaaaagaga tgtatccgga ggttgaatgg atacaggtcg
      481 ataatgccag cgctgcattt cacaaggtta aggaaggtga acttgatgct ctggtcgcga
      541 cacagttaaa ttcgcgttac atgatcgatc attactatcc taatgaactt tatcattttc
      601 ttattcctgg cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac
      661 ttaaggatat tattaataaa gcactgaatg caattccccc aagcgaagtt ctgcgcctga
      721 cggaaaaatg gattaaaatg cccaatgtga ccattgacac atgggaccta tatagcgagc
      781 aattttatat tgttacgaca ttatccgttt tattagttgg cagtagcctt ttatggggat
      841 tctacctgtt acgctcagtt cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa
      901 tatcattccg aaaagcactc tcggattcct taccgaatcc aacttatgtt gtaaactggc
      961 aaggtaatgt cattagtcat aatagtgctt ttgaacatta tttcactgcg gattactaca
     1021 aaaatgcaat gttaccatta gaaaacagtg actcaccctt taaagatgtt ttttctaatg
     1081 cgcatgaagt cacagcagaa acgaaagaaa atcgaacaat atacacacag gtatttgaaa
     1141 ttgataatgg catcgagaaa agatgcatta atcactggca tacattatgc aatcttcctg
     1201 caagtgacaa tgcagtatat atttgtggtt ggcaagatat tactgaaacg cgtgatctaa
     1261 ttaatgcact cgaggtagaa aaaaataaag cgataaaggc taccgtagca aaaagtcagt
     1321 ttctggcaac gatgagtcac gaaataagaa caccaataag ctctattatg ggcttcctgg
     1381 aacttctgtc gggttctggt cttagcaagg agcaacgggt ggaggcgatt tcacttgcct
     1441 acgccaccgg acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg
     1501 aatcgggtaa ctatcaactt caaccacaat gggtcgatat ccctacttta gtccagaaca
     1561 cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt tgcagcagta
     1621 catttcctga tcattacctg gttaagatcg accctcaggc gtttaagcag gtcttatcaa
     1681 atttactgag caatgctctc aaatttacca ccgagggggc agtaaaaatt acgacctccc
     1741 tggttcacat tgatgacaac cacgctgtta tcaaaatgac gattatggat tctggaagtg
     1801 gattatcgca ggaagaacaa caacaactct ttaaacgcta cagccaaaca agtgcaggtc
     1861 gccagcaaac aggatctggc ttaggcttaa tgatctgcaa agaattaatt aaaaatatgc
     1921 agggcaattt gtcattagaa agtcatccag gcataggaac aacatttacg atcacaatcc
     1981 cggtagaaat tagccagcaa gtggcgactg tcgaggcaaa agcagaacac cccatcacac
     2041 tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc gaccaacagg ctattactca
     2101 aacgccagct aaatctatta ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc
     2161 tacacaaagt cagtatgcaa cattatgatc tgcttattac tgacgttaat atgccgaata
     2221 tggatggttt tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta cccatctggg
     2281 ggcttacagc caacgcacag gctaacgaac gtgaaaaagg gttaagttgc ggcatgaact
     2341 tatgtttgtt caaaccgttg accctggatg tactgaaaac acatttaagt cagttacacc
     2401 aggttgcgca tattgcacct cagtatcgcc accttgatat cgaagccctg aaaaataata
     2461 cggcgaacga tctacaactg atgcaggaga ttctcatgac tttccagcat gaaacgcata
     2521 aagatctacc cgctgcgttt caagcactag aagctggcga taacagaact ttccatcagt
     2581 gtattcatcg catccacggt gcggctaaca tcctgaattt gcaaaagttg attaatatta
     2641 gccatcagtt agaaataaca cctgtttcag atgacagtaa gcctgaaatt cttcagttgc
     2701 tgaactctgt aaaagaacac attgcagagc tggaccagga gattgctgtt ttctgacaga
     2761 aaaatgacta aatagcggct cccacaatgt tcaaatgtgg gagctattta ccagcacatc
     2821 ttacatttat gatgagaact cctggcgaat ttgttcccca tgctggtcga gcgttgccgc
     2881 tcccggcata acatgcgggt ccgcgcagcc gctgattttt atcggattac ccggcatcat
     2941 tattcccccg gcttcaatca acatatttct cgcctgagtt tgtggcaatt taatggcctc
     3001 agccacactt aataacggcg cgacgggtac accaacttca tgtattctgg ctaaccaaac
     3061 ttcagctgcc tgcgttttta acgtccgctc aatatattgt ttaagaatag cctggttttg
     3121 tacgcgtaaa atattgctgc taaatcgggg atcattaacc agttccgtaa gctccagtgc
     3181 ctggcataac gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg gcttatcctg
     3241 agtattgaaa acatcaaaag gtgccatgta gggatggcga tttcccagac gttgtggtga
     3301 cttcccagtt gcgatatatg ccatcagacc atgctccaga aaactcagcg tggcatcaaa
     3361 catcgctata tcgacatgcg cccctctctg gctcttttcg cggccataaa gtgcactcac
     3421 tattccgctg aataaataga caccgccgca tagatccgca agagaggtac caacgcgcac
     3481 tggcggagca tcaggatatc ctgtttccat cattatcccg ctcattgcct gaatgatggt
     3541 atcgtaggca ggagcatctt ttagcggacc ggtatgtccg aaacctgacg atgaagcata
     3601 tatgaggcgc gggttgattt cttgaagcgt ttcccatgaa aaccccagtt tttccattgt
     3661 acctgggcga aaattctcag ctaatacatc agcttgtttg agcatattta taaatatact
     3721 tttattgtga tcattcttta aatcaagaac cacactctct ttgccatgat taataaaact
     3781 gtaatagagt gactgtccat ccacataggg accaaatgtg cgggtatcat caccatgacc
     3841 cggcggctca actttaatta cccttgcgcc catattacaa agaagttgag ttccgaaagg
     3901 tccattaagg acatgtgtca tatcgataac taataagcct tcaaacggcc ctttgctttc
     3961 attatttgtc atttgcttat gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt
     4021 tatgtaaaat caataacatg attaattatg aaaccagtcg actgacataa atccacaagg
     4081 gagccgtgac gacaaagccc agtacgctca cagccaatga cgctgtacca gtacgggtgt
     4141 agacattaaa ccggctggca ataatgattc cggagaatgc cggcggtaat gcgcctgcca
     4201 gtaccatcat ctgcagatgt tcgctgttca aatgacatgc cataccaacg agaagcagtg
     4261 ccagtggcat cagaatcagc ttcaggaagg tgttataagc aatttcggca ctgaactcga
     4321 atttatgtgc agccatagtc aaccccgcag cgaataccgc tacccctgag ttagccttcg
     4381 caatcagatt aaaggttggg tcccatgctg ccggaatttt taccccaacc aacaccagga
     4441 tcgttgccag aacaggtgcc catactactg gctcctttgc cgcagaaatt aatgcactca
     4501 gattactatt cttcttacca tccgctcctg aagaaggatt cagcaaatac agaccaatag
     4561 gaatagtaat tgcgttaaca ataatagaaa taattgctac cactaaacct gttgatacgg
     4621 aatcaccata aataggattg agaactgcaa accccaggaa tccaatggta ggtgaacctg
     4681 caattaatgc acatacagct gcttctgcat gggtacgttt aaaaaatttg tagcaaccga
     4741 accaggagaa aaagaaacat ccgacaataa ccacaagtga tacaagggtc agacgagtgt
     4801 ccgcaaaaat catttccctg tttgcccgag taatagatac aaatagagcc gcaggaagcg
     4861 cgtagtttaa taccagttta ttaaatgccc gagcttgatc ttctgaaaat gtttctcgtc
     4921 tgccgctaaa ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa
     4981 atgttagcat aatagtattt ccgataaggg tcccctcacc atcgcataat gagttggcaa
     5041 gaggagacga tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat
     5101 ggccactttc agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg
     5161 actgaatacc ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa
     5221 cgccacgaaa cgcatccatt aatttgtcat accttgcatg gtgcaacaga tccgttggtg
     5281 atggtgcacc agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa
     5341 aaataacgat cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc atcccactga
     5401 aaccaaaagc actatcacct tcaatggcga caaccggaga accagaagtc acgctagcac
     5461 cgatggcata gcccataccg atgcccatga caccccaggt accacaatcc agacgacgac
     5521 gtggtttata catatcaata atatttcgtg cattatccag ggtatttgca ccttcattaa
     5581 ctaaataaat atcctggttc tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt
     5641 aatttaatgg ttgagtatct gtacttaatt tttcatgcat tttttgtgca ttttgctgct
     5701 tgtggatatt taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt
     5761 ctgccagcat accttgcata ctggatgcaa tatcaccaac gactggcaca gcaatggggc
     5821 ggttgctgtc aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc
     5881 atcctttttt accgtgtgcc aataaccaat tcagtcgtgc accaacaaga atgacaacgt
     5941 cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc gtatcttcaa
     6001 ggatcccttt cgccatagac attggcagga atggaatctg agcactttca ataaattcac
     6061 gaagctgttc atcagcttgt gaatacgccg cgcctttgcc aaggataatt aatggccgtt
     6121 cagcttttgc taaaagcgaa attgcgctag tgactgactt cgggcatggc aataatgctg
     6181 gcgacggatt ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg
     6241 ccaggacatt tgctggcaaa tcaagataaa ctccgccagg gcgacccgat acagagaccc
     6301 ggatagcgcg tgccaatgca atgccaagat cctgcggctg attaacgcga aatgctgctt
     6361 tggcatacgg ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt
     6421 cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg
     6481 cgttggccaa tgcggtcaaa ccattgagga atcctggcgc agaaactgtc aggcagatcc
     6541 ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac
     6601 gaaaaccaat ataacgaatg ccttccgcct gggcatggcg tgccatatcc gtcacaggaa
     6661 tacctacaac accataaata gtgtcaatat tattctgttt taatgcttca acgatgatat
     6721 gcataccatc tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa
     6781 attgggagcc agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat
     6841 ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg
     6901 cgcagcttta atatccggcg taaaggcaga gaatttcatt ggacaaccaa cggtcagata
     6961 ttttccacgc aacggttgtt ccacttcaac aacactgcca ctttggcgca aagagggatc
     7021 aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga atatcaaact gagtcaaata
     7081 ggccaccgct tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc
     7141 gaaaatatgt ggctgtcgtg catgggctgt actgtatgcc ggatcggtaa tccattctgg
     7201 tttgccgatg gctttacagg tgttttccca gttttgctcc tgaatagtga aataaatata
     7261 ggcgttagga tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccacc
     7321 acctgcatta ccaccgcggg gaactgcatc accaaatgta ccattcggat actgcgggta
     7381 ttcttccaga taacccaatt tatcgagacg ctgctggtca cgtaatttca cgcggcaaag
     7441 gttcaatacg gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg
     7501 atgcagcaaa gcagcaagta aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa
     7561 cgctgcagcg cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg ccgcgccacc
     7621 cgctgcctga gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa
     7681 ccctttgatc gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa
     7741 gcccatgtga tcaatggccc ctggatgaaa gttctcgact aagatatcag cttcgcggat
     7801 cagcttttcc attacctctt tgccttccgc tgttttggta tttaactcaa tagaacgttt
     7861 gttactgtta agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg
     7921 acgcgttacg tcaccaacgc cgggacgttc aattttaata acgtcagcgc caaaccaggc
     7981 cagcatttga gtacaagatg ggccagattg cacaccggtg aaatcgagaa ctttaattcc
     8041 ttgaagtgga
//
LOCUS       DAZFAH010000194         8021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFAH010000194 DAZFAH010000000
VERSION     DAZFAH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.196"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..2131
                     /gene="iha"
                     /locus_tag="ACHJWB_004014"
     CDS             41..2131
                     /gene="iha"
                     /locus_tag="ACHJWB_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4315561.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2349..2499)
                     /locus_tag="ACHJWB_004015"
                     /pseudo
     CDS             complement(2349..2499)
                     /locus_tag="ACHJWB_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2711..3031)
                     /locus_tag="ACHJWB_004016"
                     /pseudo
     CDS             complement(2711..3031)
                     /locus_tag="ACHJWB_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3454..4029)
                     /locus_tag="ACHJWB_004017"
     CDS             complement(3454..4029)
                     /locus_tag="ACHJWB_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4315562.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4098..4676)
                     /gene="terD"
                     /locus_tag="ACHJWB_004018"
     CDS             complement(4098..4676)
                     /gene="terD"
                     /locus_tag="ACHJWB_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4315563.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4725..5765)
                     /gene="terC"
                     /locus_tag="ACHJWB_004019"
     CDS             complement(4725..5765)
                     /gene="terC"
                     /locus_tag="ACHJWB_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4315564.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5788..6243)
                     /locus_tag="ACHJWB_004020"
     CDS             complement(5788..6243)
                     /locus_tag="ACHJWB_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4315565.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6266..7423)
                     /locus_tag="ACHJWB_004021"
     CDS             complement(6266..7423)
                     /locus_tag="ACHJWB_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4315566.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7423..8004)
                     /locus_tag="ACHJWB_004022"
     CDS             complement(7423..8004)
                     /locus_tag="ACHJWB_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4315567.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2026 a         2046 c         2022 g         1927 t
ORIGIN      
        1 tgtattgtct tgccggttaa catgatcgga gattagtaat atgcgaataa ccactctggc
       61 ttccgtagtc attccctgtc tcggattttc agccagcagc atagctgctg cagaggatgt
      121 gatgattgtc tcggcatccg gctatgagaa aaagctgact aacgcagccg ccagtgtttc
      181 tgtgattagc caggaggaat tgcagtccag ccagtaccac gatctggcgg aggctctgag
      241 atcagtagag ggtgtggatg ttgaaagtgg tacgggtaaa accggagggc tggaaatcag
      301 catccgagga atgccagcca gttacacgct gatactgatt gatggtgttc gtcagggcgg
      361 aagcagtgac gtgactccca acggtttttc tgccatgaat accgggttca tgccccctct
      421 ggccgccatt gagcgtattg aggttatcag ggggccgatg tccacactgt atggctctga
      481 tgcgatgggc ggtgtggtga atatcattac cagaaagaat gcagacaaat ggctctcttc
      541 cgtcaatgca gggctgaatc tgcaggaaag caacaaatgg ggtaacagca gccagtttaa
      601 tttctggagc agtggtcccc ttgtggatga ttctgtcagc ctgcaggtac gcggtagcac
      661 acaacagcgt cagggttcat cggtcacatc actgagcgat acagcagcca cgcgtattcc
      721 ttatcccacg gagtcacaga attataatct tggtgcacgt cttgactgga aggcgtcgga
      781 gcaggatgtg ctctggtttg atatggatac cacccggcag cgttatgata accgggatgg
      841 gcaactgggg agtctgacgg ggggatatga ccggaccctg cgctatgagc gaaacaaaat
      901 ttcagctggc tatgatcata ctttcacctt cggaacatgg aaatcgtatc tgaactggaa
      961 cgagacagaa aataaaggtc gtgagcttgt acgcagtgta ctgaagcgcg acaaatgggg
     1021 gcttgccggt cagccgcggg agcttaagga atcgaacctt atcctgaatt cattactgct
     1081 tacccctctg ggagaatctc atctggttac ggtggggggc gagtttcaga gctcgtccat
     1141 gaaagacgga gttgtccttg ccagcacagg tgaaactttc cggcagaaaa gctggtcggt
     1201 atttgctgag gatgagtggc atctcacgga tgcacttgcg ctgactgcgg gcagccgcta
     1261 tgaacatcat gagcaattcg ggggacactt cagtccgcgt gcatatctgg tctgggatgt
     1321 ggcagatgcc tggacgctga aaggcggtgt gaccacggga tataaggcac ccagaatggg
     1381 gcagctacat aaagggatta gtggtgtgtc cgggcaggga aaaacaaatc tacttggtaa
     1441 ccccgacctg aagccggaag agagcgtcag ttatgaggct ggggtgtatt acgataaccc
     1501 cgccggtctg aatgccaatg tcacaggttt tatgactgac ttctccaaca agattgtctc
     1561 ttattccata aatgataaca ccaatagcta tgtaaacagc ggaaaggccc ggttgcacgg
     1621 tgtggaattt gccggcacat tgccgctgtg gtcagaggat gtcacgctgt cactgaatta
     1681 cacctggacc cgaagtgaac aacgtgatgg tgataacaaa ggtgcgccgc tgagttatac
     1741 ccctgaacac atggtgaatg cgaaactgaa ctggcagatc accgaagagg tggcatcatg
     1801 gctgggtgcc cgttatcgcg ggaaaacacc acgtttcacc cagaattatt cgtcactgag
     1861 cgctgtacag aagaaagtgt atgatgagaa aggagaatac ctgaaagcct ggacggtggt
     1921 ggatgcaggt ctgtcgtgga agatgacgga tgccctgacg ctgaatgctg cggtgaataa
     1981 cctgctcaac aaggattaca gtgacgtgag cctgtacagt gccggtaaga gtacgctgta
     2041 tgccggtgat tacttccaga cgggatcatc aacaacagga tatgtgatac ctgagcgaaa
     2101 ttactggatg tcgctgaact atcagttctg ataataacaa aacgctatca ctgacggtag
     2161 aatacgttgc cactgcaact cctggcggaa cagtggcaac gtattaggtt aagtgcattt
     2221 ccgatccgct aatgagattt cgttaccaac aactaatatc gtcacaggaa atgcacggat
     2281 tatttttaac ttatcattta catacttgtc cagagtgtaa gcgcaccgtg aaggacgtgg
     2341 ggtaaaaatt agtttacaga gagagtgacg ttccagggca acaactcttt catgcggttg
     2401 gcaggccagg tgttgattac actgatcacg tggcgtagac acgtttccgg ctcgattccg
     2461 ttaagttttg cagctaccga tcaggctgta catcactgcc gcactatcgc tcgtcatctc
     2521 aaagtccggt ctcgtcagca ggaaggtatc attctctccc gccatttttc caggggcccg
     2581 gtcagataag tccctttgtc tatcgctgac tcctgactca taacccggtt agcagaatgc
     2641 agggtcacca cctcgccacg accaaatcca aataagtcaa ttgcaccttc tcaatcgcca
     2701 ttttgtcagt aagcgtacag cctgaactga tggtatcttc accatcaatg acaacggtga
     2761 tcgcaatttt actgacgttc gccggaacac gatccagtgc tatctcaatg ctggcctgct
     2821 gcgaaccggt aacgagcctg actgccccct caggagaaga caaattatta taaaagataa
     2881 agtcagaatc gccactgacc tttccctgag cattaagcat gaacaggcag gtatcgggtt
     2941 cgcctttaaa gccggatttg gctgggtact gaagattcag cctgatcgta gattgctgaa
     3001 ggggtatgtt ttgtccggat tgtaaattca tattaactct cctgattttt gattattatt
     3061 aatgcgcagc gtttatatat gttccctagg cttagttctg gacgctggat attcggtgag
     3121 gcgtaaatat ggtatgacac catttttcat aacgctgaag tttctatacc tgttgaattt
     3181 gaattttcat tgaccgggta tcttattttc cagggcccgt tccttcataa gtcgcaaaag
     3241 taacatatat ccgaagggca tgctgttgat atcagacacg gaatactggc tttaaccagc
     3301 aaccacagat aaacccgggg cctgcataaa aggttcatgc cagaattaac atagctctgc
     3361 tttctgcccc ccccctttcc catattcacc cgttgatagc ggatcgatac ccaaaaaaaa
     3421 ccccggcatt gccggggtca gactcagcgt cttttagaca ttgacgccgt gctgggaagc
     3481 aagcgctgac agaccaccgg caaaaccctg gccaacagct ttaaacttcc actcagtacc
     3541 atggcgatac agttcaccga agaccattgc ggtttcggtt gaggcgtctt cagacagatc
     3601 gaaacgggca atttccgtcc cgttgtcgtt gttgtaaacg cgcatgaagc tgttgctcac
     3661 catgccgaag ttttgtttac gcgcttctgc atcatagatg gtaacggcaa ataccagttt
     3721 tttgatgtct gctgagactt tggtcagatc gattttgacc tgctcatcgt cgccgtcgcc
     3781 ttcaccggta cggttgtcgc cctggtgctc tactgcgcca tcagggctgg ttttattatt
     3841 gaagaaaatg aaatgggcat ctgacagtac tttaccgtct tcacctactg cgaatacgga
     3901 agcgtccaga tcaaaaccct gaccatcggt tacacgggca tcccagccca ggccaaccat
     3961 agcgacattc atggttggtg cttctttggt cagagatacg ttgccgcctt ttacgagaga
     4021 aactgccatt tttagctcct gcaaacagtt gaatgaggct gaattaacag ccccagatat
     4081 gaaaagttac ttttcgatca ggacgcgtta atgccgtact gagcacatac agatgccaga
     4141 ccaccagcat aaccctggcc tactgcgcgg aatttccact caccattgtg gcgatacagc
     4201 tcgccgaaca gcatggcagt ctcagtggac gcatcttcgg tcagatcgta gcgagcgact
     4261 tcagtctggt tatcgtcatt aaccagacga ataaacgcac cggatacctg accaaagctc
     4321 tggcgacgag cctgagcatc gtggatggtc acaacgaaga tgatcttgtc aacttcagac
     4381 gggacggcgt ccagtttaat tttcagcgat tcatcatcac catcgccctc accggtgcgg
     4441 ttatcgccgg tgtgcgttac ggaaccgtcg gatgacgtca ggttgttata gaagatgaaa
     4501 tctgaatcgc cgcgcacttt gccgtttgag gccagcagga atgctgaagc atccaggtca
     4561 aagtcctgac cgtctgttga acgcgcatcc cagccaaggc ccaccaggac atttttcatt
     4621 gacggagctg ctttactcag ggagacgttc ccgcctttgg aaagagaaac actcataaaa
     4681 taacctcttc gattagtaat tgttcaggtt aacacttaag gggattaact ccccttttcc
     4741 tcagattcag gtttgcccgg gaacatgacg cttgcgagaa tgcccagcgc cagtacaccc
     4801 agtacaacat acaggctggt tgttgccgcg atgctgtaac catgatgcca gatgtgatcg
     4861 gtcgcattca ggccgagttt tgccgcgatg aagaacagca gcacgataac ggccttctcc
     4921 agatgaacca ggtactgttt cagtgcctca aggacaaaat acagagtacg caggcccagg
     4981 atagcaaaca tcatggcact atagacgata agcggttcac gactgacggc aatgattgcc
     5041 ggtaccgagt caaacgcgaa cattacgtcc gagagttcaa ccacagccac acacaggaac
     5101 agcggggtcg catacagcgc cgctttttta ccacggccaa tggtgacatc gctgttttct
     5161 ggtttcgcca gttcagcatc cacttccttc tggttaagta ggaaggcatg ccctctgagc
     5221 ttcggccaga tagggaagaa gcgtttaacc atacggtaag ccagatgctg ggagtaatcc
     5281 tcaatttcat catcgtcatc accgctttta agcatcatga ccgccgtcca ggcaacgata
     5341 atagcgaaga caacttcaac gtacggcccc agactcagca ggctcgtacc gatggcgaca
     5401 aagatgcccc tgaagacgat ggcaccaatg atcccccagt agagaacacg gtggcgataa
     5461 cgatccggaa cggcgaacca ggagaagatt gccatcatga cgaacaggtt atcgaccgac
     5521 agcactttct ccagcgcata acccgtgaca aacagactgg caacctcagc accgtggtgg
     5581 atatagagga aaccggcaaa tgccatcgca accacaaccc agaatacgga ccagagcgca
     5641 gcactcttca gcgagattgg cttgtcatca cggtgcataa acaggtcaat aaagatggca
     5701 ccgactgaaa gcgcaataaa aacaatgacc gtttcagtcg ggaagccgat gtgtgtggaa
     5761 accatatttt acccttaggg atgtggatca gaggccaaat tcagccgggt cgaagcccaa
     5821 cgcgatagcg atctcgcggg aggccagttt ctcgtcctga tcgaagttac cgtcactttt
     5881 cgcaacggca ataccaacac gtaaggccag ctgagcggcc tcaggctgat ctttcagcgc
     5941 caggatgtat ttcatggttt cgcccttgcc aatttcaaca tcgaaatcga agcttgaaac
     6001 cagtttattg aagaactcaa tcacctcatt ggtatcgaag accttcagct cttctgaaga
     6061 gcgcagaaag cccatcattt tctgcttttc ttccgaactt acgccgtcac tcgatacggc
     6121 aatacgggca catacagcaa cggtgccctg catgaatttt ttgtttttga aacggccaac
     6181 ctggcgggtc agttcgtcac ggcctgagtt aatggcacct ttaactttgt cgaaaaagct
     6241 catacctgtt tcctttgttg ctgtgttatt tagaaccggc gctccagcga aatccccagc
     6301 caaatgcccg gtccatttcg tcctggcctt tgaagtactg gttaattcgc tcgactttaa
     6361 ttgcgccgtt ttcgtttacg agtctggcaa tggcgcacaa tgtgcgacgg ttctcacctt
     6421 cggtcaggcg ggtctcaatg ggcggttgat ccggcacatg aatggtgact acaccatcag
     6481 tcttgtccca gctgggaaca ccttcataaa taaaggcgta aatcagcact tcgcggatat
     6541 gcttccattc acgtccattg atatgcagcc actcgccgtc tgatacatca cccgtccggt
     6601 catcgccctt gagctgaaca tacggttcat cgcgataatc accgaatgca ttaccgagcg
     6661 cctgaatgac cgatttatac ccgtcctgaa gctcaacaaa ggcccccaga tccaggtcga
     6721 ttcctttgga gccgaacata cctgcaaagc ctgatttgct actgccccgg tgccagttca
     6781 ggttgatccg gatttcaccg aagttatccc gtttggtcag gctgattgca ggcttctctt
     6841 ttgtcagcga taccttgctc aggctgattt ttgtctcaac tggcggaggg gttacaaccg
     6901 gaggagttgg ggctgcggga gccggttcgt cggcaatatt tacgccgaaa tgctctgcca
     6961 gcggtttaag tccaccgttg aacccctgcg caacgaagcg gaatttccag tcattattac
     7021 gacggtaaaa ttcccccaga attaaggctg cttcctgacg gccgctcagt tcgacactgc
     7081 cgctaaccag gccggtagcc ccttgctcca cgtcgatgga aagattacga agaccggaca
     7141 ccgtctgccc accgtcacag gtcacggtga acgcaatttt ctggacatca ggtttcagcc
     7201 ggttgagcgc gacagtaaag gttgagtact ggccttcact gaccaggctg acggtaccat
     7261 catcgttacg tggctgacca taaaacacca tgtccgcatc accctgcacc ttcccgtcgg
     7321 catacaggcg aaacgccgaa gcatccacct ggccgcccga ggtgatccgg acccgcaact
     7381 cctgggctgg aacgggggca ttgccccccg gcgtcaggtt cattagcgaa taaccgctga
     7441 aacgatatcg gagtgcatat cgtcaattgt gcggccgcga caggcatggc caagggcggt
     7501 aaagtcccag ttgccgttat tacggcgcag cgaagaaata acgatcccgg tatgtgagcc
     7561 ctgctcagtc agcttgtagc gagccagctc tttgccggtt tgatcgacaa cgcggcagaa
     7621 agcgttttcg acgtcattga acgactgacc acggaagctg tttaccgtaa acgccaggta
     7681 ttcaacgttt gcaggcaggc gggagaggtt gaccttaatc acttcatcgt caccgtcacc
     7741 ctcaccggtc aggttatcgc cactgtgcac aacagcgccg caggtggatt tcagtttgcg
     7801 gaaccagatg gtgtcaattg ttttacctgt gctgtccatt aatacacagc cagcatcgag
     7861 atcgattgag tcgttgcctc caaacaatcc tccaagaaga ccttttttct taaccggatc
     7921 ccaaccgaga ccgaaatgaa gctggcttaa tgcagatgat tctttgctga gtgataccgt
     7981 ctggttctta actaatgaaa ccatgtttta ttccttaaaa t
//
LOCUS       DAZFAH010000195         7855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFAH010000195 DAZFAH010000000
VERSION     DAZFAH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.197"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            113..202
                     /gene="kdpF"
                     /locus_tag="ACHJWB_004023"
     CDS             113..202
                     /gene="kdpF"
                     /locus_tag="ACHJWB_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4315568.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            202..1875
                     /gene="kdpA"
                     /locus_tag="ACHJWB_004024"
     CDS             202..1875
                     /gene="kdpA"
                     /locus_tag="ACHJWB_004024"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4315569.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNSGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVMTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1898..3946
                     /gene="kdpB"
                     /locus_tag="ACHJWB_004025"
     CDS             1898..3946
                     /gene="kdpB"
                     /locus_tag="ACHJWB_004025"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4315570.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASAFIPTQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3955..4527
                     /gene="kdpC"
                     /locus_tag="ACHJWB_004026"
     CDS             3955..4527
                     /gene="kdpC"
                     /locus_tag="ACHJWB_004026"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4315571.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4520..7204
                     /gene="kdpD"
                     /locus_tag="ACHJWB_004027"
     CDS             4520..7204
                     /gene="kdpD"
                     /locus_tag="ACHJWB_004027"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4315572.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEDENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7201..>7855
                     /gene="kdpE"
                     /locus_tag="ACHJWB_004028"
     CDS             7201..>7855
                     /gene="kdpE"
                     /locus_tag="ACHJWB_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4315573.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     "
BASE COUNT         1592 a         2071 c         2345 g         1847 t
ORIGIN      
        1 ggcttttgcc atttttatac tttttttaca ccccgcccgc agatttttgc gaaatctttg
       61 cagccagaat tctacccttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc
      121 aggcgtgata accggcgtat tgctggtgtt tttattactg ggttatctgg tttatgccct
      181 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgatcgc cacgttttta
      241 ctggtgttaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
      301 attcctcttc ccggtacaac gggcgttgag cgcgtacttt ttcgcgcact tggcgtctct
      361 gaccgtgaga tgaactggaa gcaatatctc tgtgccattc tcggcctgaa catgctgggg
      421 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag
      481 ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc
      541 aactggcaat cttacagcgg tgaaaccacg ttgagctatt tcagccagat ggctggctta
      601 acggtgcaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cctcatccgt
      661 gcgtttaccc gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc
      721 acgttatggg tgctcgtccc tgtggcgttg ttgatcgcac tattttttat tcaacaaggt
      781 gcgctgcaaa actttcagcc ttatcaggct gtgaataccg ttgaaggagc gcaacagctg
      841 ttacccatgg ggcctgtagc ttctcaggaa gcgatcaaga tgctcggtac taacagcggt
      901 ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
      961 gtgcagatgc tggcgatctt cttgatccca acggcgctgt gctttgcctt tggtgaagtg
     1021 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     1081 tgcgtaggcg tggtgatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc
     1141 gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     1201 ctgtttgcgg tcatgactac ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg
     1261 tttaccgctc tcggtggcat ggtgccgatg tggctgatgc aaattggtga agtggtgttc
     1321 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     1381 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     1441 atgaaactga ctgcactggc gattctggtt accccgacgc tggtgctgat gggcgcggcg
     1501 ctggcaatga tgaccgacgc cggacgtagc gccatgctca accctggccc gcatggtttt
     1561 agcgaagtgc tgtacgccgt gtcatccgcc gctaacaaca acggcagcgc ctttgccgga
     1621 ttaagcgcca actccccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     1681 ttcggggtga ttatcccggt gatggcaatt gccggttcgc tagtgagtaa aaagagccaa
     1741 cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc
     1801 accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg
     1861 gaatatctct cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct
     1921 attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca
     1981 ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg
     2041 tattagcatc gctatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat
     2101 tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg
     2161 ccgcagtaaa gcgcaggcca acagtctgaa aggggtgaaa aaaactgcct ttgcccgcaa
     2221 gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa
     2281 aggtgacatc gtgctggtgg aagctggcga tattatcccc tgcgatggtg aagtcattga
     2341 aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga
     2401 atccggcggc gattttgcct ccgtcaccgg cggtacgcgt attctttctg actggctggt
     2461 gattgagtgt agcgttaacc ccggagagac atttctggat cggatgatcg cgatggtgga
     2521 aggagcgcag cgacgtaaaa cgccgaacga gattgccctg accatcctgc tgattgccct
     2581 gactatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa
     2641 tgcagtcagc gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg
     2701 cgggctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg caaatgtgat
     2761 tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc ttctggataa
     2821 aactggcacc atcacactcg gtaaccgtca ggcgtcggcg tttatcccca cgcagggcgt
     2881 ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga
     2941 aggccgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca
     3001 gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat
     3061 cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccatg ttgaggctaa
     3121 cggcggtcac ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcagggagc
     3181 cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat
     3241 cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc aaaatgggca ttaaaacggt
     3301 gatgattacc ggcgataacc gtctgactgc cgccgccatt gctgcggaag cgggcgtcga
     3361 tgattttctc gccgaagcga caccggaggc caagctggca ttgattcgtc agtatcaggc
     3421 ggaaggccgt ttggtggcga tgaccggcga cggcaccaat gatgctccgg cgctggcgca
     3481 ggcagatgtc gcggtagcga tgaactccgg tactcaggcg gcgaaagaag cgggcaatat
     3541 ggtcgatctc gactctaacc cgaccaagtt gattgaggtg gtgcatattg gcaaacagat
     3601 gctgatgacc cgtggctcgc tgaccacctt cagtattgcc aacgatgtgg cgaaatactt
     3661 cgccattatt ccggcggcgt tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat
     3721 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     3781 cgtctttttg attcctctgg cattaaaagg cgtgagttat aaaccgctta ccgcttctgc
     3841 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     3901 tatcaaagtc attgatttac tgctgaccgt ttgcggtctg gtgtgaggtt taccatgagt
     3961 ggattacgtc cggcattatc gacatttatc tttctgttat tgattactgg cggtgtttac
     4021 ccgctgctga ccaccgcgct ggggcaatgg tggtttccct ggcaggccaa cggttcgttg
     4081 atccgtgaag gtgatacagt gcggggttcg gcattaatcg ggcagaattt tactggcaac
     4141 ggctattttc atggtcgccc gtcggcaacg gcagaaatgc cctataatcc acaggcttct
     4201 ggcgggagca atctggcggt cagtaaccct gagctggata aactaatagc cgcacgcgtt
     4261 gctgcattac gggccgctaa cccggatgcc agcgcgagcg ttccggttga actggtgacg
     4321 gcatcggcaa gcgggctgga caataatatc accccgcaag cggcggcctg gcaaatccca
     4381 cgcgtggcga aagcgcgtaa tctcagcgtt gaacagctca cgcaactgat cgcaaaatac
     4441 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     4501 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg atcgtctgct
     4561 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg
     4621 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     4681 tattgtggtt ggcgtggtag aaacccacgg acggaaagat accgccgcca tgctggaagg
     4741 gctggctgtt ctgccgccaa aacgcctggc gtaccgtggg cggcatatca gcgagtttga
     4801 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     4861 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     4921 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     4981 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5041 cgacgacgta gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5101 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     5161 tctgatcgcc ctgcgtgaac tggcactgcg ccgtactgcc gatcgcgtag atgagcaaat
     5221 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtgtgg cacacgcgcg acgcgatcct
     5281 tttatgcatc gggcataaca ccggcagtga gaaactggtc cgcgcggcgg cgcggttggc
     5341 ctcgcgcctg ggtagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt
     5401 accggaaaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg aactgggcgc
     5461 ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga
     5521 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     5581 aacgtttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     5641 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctctt ttaaagacaa
     5701 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     5761 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctact
     5821 tggcgtggtg gtggtggcgc tattttatgg acgctggcct tctgtggttg ctaccgtcat
     5881 taatgtggtg agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga
     5941 tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct
     6001 tactgctggc gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca
     6061 cttatatgaa atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac
     6121 cagcgaacaa tttattgcct ccacgtttca tgcccgcagt caggtgttgt tgcccgatga
     6181 caacggtaaa ttgcagccgt taacacatcc gcaaggaatg acgccgtggg acgatgccat
     6241 cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca cattacccgg
     6301 cgtgccgtac caaattttgc cgttaaaaag cggtgagaaa acctacgggc tggtggtggt
     6361 ggagccgggg aatctgcgcc agttgatgat cccggaacaa cagcgcctgc tggagacatt
     6421 tacgttgtta gtcgccaatg cccttgagcg gctgacactg accgccagcg aagaacaggc
     6481 gcggatggca agcgaacgtg aacagatccg caacgctctg ctggcggcgc tttcgcatga
     6541 tttacgcacg ccgctcacgg tgctgttcgg tcaggcagaa atcttaacgc tcgatctggc
     6601 aagcgaaggc tcgccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac
     6661 tacccgactg gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt
     6721 gaagaaagag tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc
     6781 gggtttatcg tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga
     6841 cgggccactc tttgaacggg tgctgattaa tctgctggag aacgcggtga aatatgcggg
     6901 tgcgcaggcc gaaattggta tcgatgccca cgttgaggac gaaaatctac aactggatgt
     6961 ctgggataac ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc
     7021 tcgcgggaat aaagagtcgg cagtaccggg tgtagggctt ggactggcaa tttgtcgggc
     7081 gattgtggat gtacacggcg gcaccattac cgcgttcaac cgaccggaag gtggtgcctg
     7141 ttttcgtgtt acacttcccc agcaaaccgc tcctgaactt gaagactttc atgaggatat
     7201 gtgacaaacg ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg
     7261 ctggagggcg acgggatgcg cgtctatgag gccgaaacgc tgcaacgcgg cttgctggaa
     7321 gcggctaccc gtaagccaga tttgattatt ctcgatctcg gcctgcccga tggtgatggg
     7381 attgagttta tccgcgacct gcgccagtgg agcgcggtgc cggtgattgt gctttccgca
     7441 cgcagcgaag agagcgacaa aatcgccgcg ctggatgccg gagcggatga ttatctgagt
     7501 aagccgtttg gcattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc
     7561 gccaccgccg cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc
     7621 cgcgtgattc accggggtga ggaagaggtg catctcacgc cgattgagtt ccgcctgctg
     7681 gcggtgctgc tgaataatgc cgggaaagtg ctcacccagc gccagttact caatcaggtg
     7741 tgggggccaa acgcggtcga acacagtcac tatttgcgta tttatatggg acatctgcga
     7801 caaaaactgg aacaggatcc cgctcgcccc cgccatttca ttactgaaac cggta
//
LOCUS       DAZFAH010000196         7801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFAH010000196 DAZFAH010000000
VERSION     DAZFAH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.198"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..512
                     /gene="lpfA"
                     /locus_tag="ACHJWB_004029"
     CDS             <1..512
                     /gene="lpfA"
                     /locus_tag="ACHJWB_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO4315574.1"
                     /translation="FALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPDTQKQE
                     VVLGQVNKSVFTAPGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKLISVST
                     EPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTIGYGNA
                     EVDFNLTYN"
     gene            596..1282
                     /gene="lpfB"
                     /locus_tag="ACHJWB_004030"
     CDS             596..1282
                     /gene="lpfB"
                     /locus_tag="ACHJWB_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO4315575.1"
                     /translation="MKRISLVVLAFLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1306..3837
                     /gene="lpfC"
                     /locus_tag="ACHJWB_004031"
     CDS             1306..3837
                     /gene="lpfC"
                     /locus_tag="ACHJWB_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO4315576.1"
                     /translation="MHWMHLPLYHSRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKTSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     LITARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3848..4903
                     /gene="lpfD"
                     /locus_tag="ACHJWB_004032"
     CDS             3848..4903
                     /gene="lpfD"
                     /locus_tag="ACHJWB_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO4315577.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4909..5433
                     /gene="lpfE"
                     /locus_tag="ACHJWB_004033"
     CDS             4909..5433
                     /gene="lpfE"
                     /locus_tag="ACHJWB_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4315578.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5686..7377
                     /gene="eptB"
                     /locus_tag="ACHJWB_004034"
     CDS             5686..7377
                     /gene="eptB"
                     /locus_tag="ACHJWB_004034"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4315579.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7469..7545
                     /locus_tag="ACHJWB_004035"
     tRNA            7469..7545
                     /locus_tag="ACHJWB_004035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7503..7505,aa:Pro,seq:cgg)
     gene            7580..7713
                     /locus_tag="ACHJWB_004036"
     ncRNA           7580..7713
                     /ncRNA_class="other"
                     /locus_tag="ACHJWB_004036"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2026 a         1796 c         1979 g         2000 t
ORIGIN      
        1 tgtttgcctt atctgcgctt gcgctgacct caacttccgt attcgcagct gatgcgggcg
       61 acggtactgt taaattcact ggtgagattg ttgacgccgc ttgtgttgtg tctccagaca
      121 ctcaaaaaca agaagttgtt ctgggacagg tgaacaagtc tgtgtttact gctcctggtg
      181 ataaatccac agcaactccg ttcaaaatca aactggaaaa ctgcgatatc tccactttca
      241 aaaatgtgga aatcagcttt aacggtgttg gcgatgcaga taacagcaaa ctgatttctg
      301 taagcactga accaggtgct gcaactggcg taggtatcgg tatttatgat aacaccaata
      361 ccttggttga cctgaatacc ggtaaatctg ctaccgtcct gaaagaaggc cagaccgttc
      421 tgtatttcac cgctaactat gtctctacta aagatgctgt aaccatcggt tacggtaacg
      481 ccgaagtcga cttcaacctg acttacaact aatcgatcct gacggaacga tcatctctca
      541 atggcaacag agactctgtt gccattattc acaccaatga ctcagggacc gaatcatgaa
      601 acgtattagc cttgtcgtgc tggcattttt aatgaccgca caacagtgtt ttgctggtgg
      661 cgtagcctta agtacgacgc gtgttattta tgatggcggc aagaaagaag cgtcgctgac
      721 cgttgaaaac cataataaaa atgaagagtt tttgattcag tcatggattg atgacgtgaa
      781 tggcaataaa aaaacaccat ttattattac accgccgtta ttcaaacttg atcctgataa
      841 aaataacgtc ttacgtatcg tcaatattga agatacttta cctaaagatc gtgaatccgt
      901 ttactggatt aatgtaaaag ccattcctgc gaaaagcgct gaaaatgaaa ataaaaacgt
      961 cttacagata gccgtgcgca cccgtctgaa gttgttctac cgtccaaaag gtttgccggg
     1021 aagcaacctc gaggcaatga aacagttacg tttccaacgt caaaataacc aaattgtggt
     1081 taataacgac tccgcttata acctgacttt taatcagttt tccatcaatg gacaaaagat
     1141 tgaacgggcg ggaatggttc tcgcgaaagg aaagttaaac attaaccttc ctgccggtac
     1201 aggcaacgcg aacgaagtga aatacagcat tattaatgat ttcggtgttg ctggcgaaat
     1261 gctgacgaag ccgattaact gatcctttta caaggcgctt ttactatgca ttggatgcat
     1321 ttacctctct atcactccag agcgcatttt tccttctcac tgctggcatt aacgattgcc
     1381 agcgcgttac ctgcgtatgg cggaaaattt aaccctaaat ttctcgaaaa cgttcagggt
     1441 atcgatcaac acatcgactt gtcggtatac gattcacctg tcggtcagca aatccccggt
     1501 aaataccgtg tttcggtgtt tgtaaacgaa gaaaaaatgg cttcccggac gctggatttt
     1561 agtaccgcca gcgaagcgaa acgcaaagcc agcggtgagt cgcttatgcc ttgccttagc
     1621 cgggtgcaac tagaagaaat gggcgtgcgc gttgacagct ttccggcgct gaagacgtca
     1681 ccaccagaag cctgcgtagc gtttgatgaa atcattcctc aagccaccag ccgctttgat
     1741 tttaatactc agacgctgca tctttccttt ccacaggcgg caatgttgat taccgctcgc
     1801 ggcacggttg atccttctcg ctgggacgaa ggaatacctg cgctgttgct ggattacagc
     1861 tttagtggca gcaatggacg caatgaaggt acaggttcat cttcagattc gaccagtgac
     1921 agttattacc tgaacctgcg cagcggtttg aacgttggtc cgtggcgttt acgtaataac
     1981 agtatctgga accgtaccga tggcaaaaac caatgggata acgttggtac atcgttaaac
     2041 cgggcgatta ttccactcaa gtcacagata acattgggtg ataccgctac gcctggagag
     2101 atttttgaca gtgtacaaat gcgcggggcg ttactggcat ccgacgatga aatgttgccg
     2161 gatagccagc gtggctttgc tccggtggtg cgcggcatcg ccaaaagcaa cgctgaagtg
     2221 agtatcgaac agaacggata tgttatttat cgtacctttg ttcagccggg cgcatttgag
     2281 attaacgatc tctatgcaac ctccggcagc ggcgatctga cggtgatcat taaagaagcc
     2341 gacggaagtg aacaacgttt tatccagccg ttttcggcag tggcgatttt ccagcgtgaa
     2401 ggttatctga aatacagcct ggctgccggt gagtatcgtg ctggtaatta cgacagcgat
     2461 aaaccacatt tcgggcagtt taccgccatg tacggcttgc cgtggggtat gaccgcctac
     2521 ggtggtgcat tgttgtcggc agattacaac gctttagctc tgggcctggg gaaaaacttc
     2581 ggtaccattg gcgcagtatc ggtggatgtc actcaggcga aaagtcagtt acgcaacaat
     2641 gaaaaagagg aaggccagtc ttatcgcttc ctctattcga aaagttttga gggtggcact
     2701 gacctgcgtc tgttgggcta caaatactca accagcggct actacacctt ccaggaagca
     2761 actgatgttc gcagtgacgc cgacagtgat taccgccgtt atcacaagcg cagccagata
     2821 cagggcaata ttacccagca actgggagac tacggctcgg tttatttcaa tatgacgcag
     2881 caggattact ggaacgttga cggtaaggaa aactccttaa gcgccggtta tcacggtcac
     2941 attggtcggg tgaattacag cgtggcctat acctggacga gaagtccgga atgggaagaa
     3001 gacgatcgct tgtggtcatt cagtgtttcc atccctctgg ggggcgcgtg gagcagttat
     3061 cgcatgacta ccgaccagaa tggcaagacg tcgcaacagg cctctgttag cggcacgtta
     3121 ctggaagatc gtaacctgag ctataacgtt cagcagggtt acaccagtaa tggcgtaggc
     3181 tacagtggca gcgtcaacat ggggtatatg ggtggctctg gtaacattga cgtaggctat
     3241 aactattcta aagataacca acaggttaac tatggcgtgc gcggcggtgt catcgttcac
     3301 agtgaaggta taacgctttc gcagccttta ggcgaaagtc tggcgattgt ttctgcaccg
     3361 ggcgcgcgtg gtggacatgt ggttaacagc agcggcgtag aagttgactg gatgggcaac
     3421 gccgtggtgc cttatctcac accatatcgt gaaaccattg tggaattgcg tagcgacacg
     3481 ttggggcaaa acgttgaact acaggaagcg ttccagaaag tcgtgcctac tcgtggtgca
     3541 gttgtgcgtt cccgttttga tacccgcgtc ggttaccgtg tgctgatgag cttaaaacag
     3601 gcgaatggca acgcagtacc gtttggtgca accgcagcgt taattgatga gagcaaacct
     3661 gccagcagca ttgtgggtga agaaggacag ctttatatca gcgggatgcc agaagagggc
     3721 gaattgcagg tgagctgggg gaacgagcag gcgcaacgtt gccgtgtgcc attccgcttg
     3781 ccagagaaca aggacaatgc ggctatcgtg atggttaatg cggtatgcga gaaataagga
     3841 tatttagatg aaaattaaaa atttgttggc aggtatggtg ctgttaggga cgagcacttt
     3901 tgctggaaat atatgggctg cggattgggg accatgtcag acatcaaatg gggtggccca
     3961 tgagtatagt tttgatttcg tacagactat ccaggtacct tcagaaaata aggctgggaa
     4021 aatattaact cagccctttg ctttaggtac aaaatattcc gcttattgtg aatgcccgga
     4081 tcccattcca gataatggag ttgtgactta ttttaaaggt gtaacgcttc tgcctgagcc
     4141 tggaactgta gatgggtact ataaattcaa taattacatt gatattctca ccaaaatata
     4201 tgtctacaca caattagata tcccggtgcc ttttaccgat cgaagcaatg gcacggcaca
     4261 gaaagaatgt acgccctata ctgctaacaa ttgggggaca ggtggtaaag gaagtatcag
     4321 tatttatatt tcacatcctt ttgttgggca aatgattatt cctaaaacac gagttgcgtc
     4381 cctctttggg acaaaaaaga aaggtgttta caatgatagt cagcccatgg ccaatgtcag
     4441 tatttccggg agtattacag ttactcaagg ctgtgaactt gccgctggca ccgtactgga
     4501 tattccattc ggagaatatc aggcccatga ttttaaaggg cgtgccggac aaccaccaca
     4561 aaatgttcag aaggtacaaa aagaattaag ctttgactgc aataatattt cagatggcgt
     4621 caaaatttat ctcagtattg atgcaacacc aaatactgcc tatcccagcg caattgattt
     4681 aggcaacgcc gatgttggtg cagtcattga ggacggtaaa ggtaatattc ttaagccgaa
     4741 tgacagtaat agcttattag aaatgaaccc aggctcgctg tatgaagatg tcaaacgcaa
     4801 agcgacaacc acaattaccg cttatcccgt tagcactacc ggaaaattac ccgctgccgg
     4861 ggattatagc ggtattgcta ccatacatgt cgaactggag taacagtcat gaagcgaaca
     4921 atacttgcgt tatgcctgct gcctctttcc ttttcgtcat tcgcggcaac cacggattta
     4981 gggccgaaag gaacattgaa attcaccctt aaaatcagcc agggtgcttg tgaactggtg
     5041 aaagacagcg ttgaggttga catgggcacc gcggttctca aaaagccggt aagaatcgga
     5101 acggaaatta atcccactcc tttttccatc ggcctgaaaa actgtagcga agtggtcaga
     5161 gcctacgtga ctatggacgg tacacccaat gcagacgact caaatctttt tgcgctggat
     5221 gcgggtggcg ctacaggcgt tgggctgaaa atcaaaaccg ctgctggagt acaacagttc
     5281 ccggaaaata caacgccgac accggttgag tttgcaatta acggtgatgg cgcacatcaa
     5341 ctgaactata tcgccagcta cgtgcctatt cgcgccgatg caactattgg tcgcgcagat
     5401 gcaacggtcg actttactgt acagtacgaa taatttcaca tcttaagggc cagttaactg
     5461 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     5521 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     5581 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
     5641 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
     5701 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
     5761 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
     5821 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
     5881 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
     5941 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
     6001 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
     6061 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
     6121 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggc ggtcgtcgta
     6181 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
     6241 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
     6301 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
     6361 gataataatt cattgcttaa tccggcgaag aaattcactt atcaggcacc gcaaaacgtt
     6421 gatgacactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
     6481 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
     6541 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
     6601 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggtgctg
     6661 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
     6721 aacacgatgg cggacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
     6781 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
     6841 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
     6901 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
     6961 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
     7021 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
     7081 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
     7141 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
     7201 aatgcgcagg catttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
     7261 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
     7321 aacgaaaaca acaactggtg tcacatcccg caggcaaaag aggcagcggc taactaagcg
     7381 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
     7441 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
     7501 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
     7561 ctcagcgagc aggctttttt gcatttgaag cccatgagga tgagaacctc cgggggcggg
     7621 gaggttcgac tcgagcgaag cgagagaacg ttgcgccagc aacggcccgc agggcgagcc
     7681 acgaagtggc gagtaatcct ctatcaccga ccaaattcga aaagcctgct cagcgagcag
     7741 gctttttttg catttgtagc ccatgaggat gagaacctcc gggggcaggg aggttcgact
     7801 c
//
LOCUS       DAZFAH010000197         7775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFAH010000197 DAZFAH010000000
VERSION     DAZFAH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.199"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(93..917)
                     /locus_tag="ACHJWB_004037"
     CDS             complement(93..917)
                     /locus_tag="ACHJWB_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4315580.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(970..2688)
                     /gene="proS"
                     /locus_tag="ACHJWB_004038"
     CDS             complement(970..2688)
                     /gene="proS"
                     /locus_tag="ACHJWB_004038"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4315581.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFSLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMNDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHJWB_004039"
     CDS             complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHJWB_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4315582.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MPVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEGTGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHJWB_004040"
     CDS             complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHJWB_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4315583.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHJWB_004041"
     CDS             complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHJWB_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4315584.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4880..5533)
                     /locus_tag="ACHJWB_004042"
     CDS             complement(4880..5533)
                     /locus_tag="ACHJWB_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4315585.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHJWB_004043"
     CDS             complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHJWB_004043"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4315586.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHJWB_004044"
     CDS             6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHJWB_004044"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4315587.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            7684..>7775
                     /locus_tag="ACHJWB_004045"
     rRNA            7684..>7775
                     /locus_tag="ACHJWB_004045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1931 a         1989 c         1970 g         1885 t
ORIGIN      
        1 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
       61 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
      121 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
      181 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
      241 tgcgccagtg taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa
      301 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
      361 acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
      421 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
      481 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
      541 agcttatcac tatgcttcat cgctttttta agggaaacga tctggacgcc agcgcctgtc
      601 gcttctgcaa cgttattatc acccagatag attgcaacgt gactcacaga ggaagtgctg
      661 aagacgcgga ttccaaatga agttacccca aggcttgagg agaacagcaa atcgccgggt
      721 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
      781 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
      841 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac
      901 gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc
      961 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
     1021 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
     1081 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
     1141 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
     1201 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
     1261 cacctggaac ggcgcgatcg cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc
     1321 aatcgccgca gctactacac gcgttacccc gataccgtag caacccatag tcaggatttg
     1381 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
     1441 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
     1501 gcttggatcg ccagcgacca cgttgcggat atcagcaatt tccggggtag cgacatcgcg
     1561 atcccagttg ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcatt
     1621 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
     1681 acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
     1741 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
     1801 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
     1861 tttctcaatc ggcagactga actgttcaac cagttccgcg atggttttcg cgttcggcgt
     1921 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
     1981 tgccagctca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
     2041 gctttgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
     2101 ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
     2161 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
     2221 aaggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
     2281 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
     2341 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
     2401 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
     2461 tggctgaacc accggcatcg acacctcgat tgcaccggcg ttgttcatct cttcacgcac
     2521 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
     2581 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
     2641 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
     2701 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
     2761 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
     2821 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgcag ccagacggca
     2881 taggttttgc ccgttccctc acctttacga taggccgggc gcgggtcctg cgccagtact
     2941 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
     3001 acctctgcgg taaaactcac cggcatttct gccgctggcg cactttgcgc atagctggca
     3061 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac caccggcgta
     3121 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
     3181 tctttcagct ctaccagcga cataccaatt ggattaggac ggaaagtaga gcgtgttgcg
     3241 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
     3301 ccttccatcg tttgatgaaa gacgaaaagg atccacaaat ggctgaacgc ttccaggccg
     3361 cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt
     3421 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
     3481 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagaa ccgatacata
     3541 cagcctgacg atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg
     3601 cattggcctt cattttcgag gcgttgattt gcatccgctt acgtgcggtt ggaatgctcg
     3661 gcggagagtc ctgattggag gcctggcaag aatcgccact gacttcaccg agatcgcgga
     3721 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
     3781 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
     3841 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
     3901 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
     3961 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
     4021 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
     4081 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
     4141 gcgttacgat caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg
     4201 ctggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
     4261 cgtcgtccag agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg
     4321 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
     4381 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
     4441 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
     4501 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
     4561 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
     4621 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
     4681 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
     4741 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
     4801 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
     4861 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
     4921 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
     4981 tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
     5041 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
     5101 ccggcaacgc ttccggtaac agcactttac ggacgatctg cattggcgtg gcacccattg
     5161 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
     5221 caataaacgg tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg
     5281 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
     5341 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
     5401 gccccggacg cgtgacataa agcagaacgc caaccggcag accaatcaca aagccaaaaa
     5461 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca
     5521 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
     5581 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg
     5641 ccaccggcgt agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
     5701 gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc
     5761 acgcagtcag taaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga
     5821 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
     5881 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
     5941 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
     6001 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
     6061 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
     6121 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
     6181 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
     6241 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
     6301 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg
     6361 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
     6421 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
     6481 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
     6541 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa
     6601 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
     6661 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
     6721 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
     6781 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
     6841 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
     6901 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
     6961 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
     7021 ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgactg ccgtaaacca
     7081 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
     7141 atggtgggcg ataaattaga agatatgcag gcagcggctg cggcgaacgt gggaacaaaa
     7201 gtgctggtgc gtacgggtaa acctattacg cctgaagcag aaaacgcggc agattgggtg
     7261 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
     7321 ttaaaagatg agcggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
     7381 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
     7441 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata
     7501 cgccacctcg cgacagtgag ctgaaagctg cgtcgcaact gctctttaac aatttatcag
     7561 acaatctgtg tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata
     7621 ccaagtctca agagtgaaca tgtaattcat tacgaagttt aattctttga gcatcaaact
     7681 tttaaattga agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg
     7741 caagtcgaac ggtaacagga agaagcttgc ttctt
//
LOCUS       DAZFAH010000198         7727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFAH010000198 DAZFAH010000000
VERSION     DAZFAH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.200"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..690
                     /gene="ecpR"
                     /locus_tag="ACHJWB_004046"
                     /gene_synonym="matA"
     CDS             148..690
                     /gene="ecpR"
                     /locus_tag="ACHJWB_004046"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO4315588.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            765..1352
                     /gene="ecpA"
                     /locus_tag="ACHJWB_004047"
                     /gene_synonym="matB"
     CDS             765..1352
                     /gene="ecpA"
                     /locus_tag="ACHJWB_004047"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO4315589.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1410..2078
                     /gene="ecpB"
                     /locus_tag="ACHJWB_004048"
                     /gene_synonym="yagY"
     CDS             1410..2078
                     /gene="ecpB"
                     /locus_tag="ACHJWB_004048"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO4315590.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2104..4629
                     /gene="ecpC"
                     /locus_tag="ACHJWB_004049"
                     /gene_synonym="yagX"
     CDS             2104..4629
                     /gene="ecpC"
                     /locus_tag="ACHJWB_004049"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO4315591.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNIYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4619..6262
                     /gene="ecpD"
                     /locus_tag="ACHJWB_004050"
     CDS             4619..6262
                     /gene="ecpD"
                     /locus_tag="ACHJWB_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO4315592.1"
                     /translation="MRVNLLIKMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6231..6941
                     /gene="ecpE"
                     /locus_tag="ACHJWB_004051"
     CDS             6231..6941
                     /gene="ecpE"
                     /locus_tag="ACHJWB_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO4315593.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7254..7583
                     /gene="ykgJ"
                     /locus_tag="ACHJWB_004052"
     CDS             7254..7583
                     /gene="ykgJ"
                     /locus_tag="ACHJWB_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4315594.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
BASE COUNT         1996 a         1980 c         2006 g         1745 t
ORIGIN      
        1 tctcgtttaa cctttattga tatgtactac gtatcttatt tacttccggt ttactaagga
       61 aactgaatgc acctgtaaaa attacaggtt tggaaagtag tgacatggca aagtgattac
      121 agtagggact atgaggttaa aaaccatatg gaatgtcaaa accgttctga taaatacatc
      181 tggtctcccc atgacgccta cttctataaa ggactatctg aactgattgt ggatatcgac
      241 agattaattt atctatcgtt ggagaaaatt agaaaagatt tcgtgtttat caatctcaat
      301 acggattctt tatctgaatt tataaaccgt gataatgaat ggttatccgc ggtaaagggg
      361 aaacaggtcg tattgattgc ggccagaaag tcagaagcct tagcaaatta ttggtattac
      421 aacagcaata ttaggggcgt ggtatacgca ggactgagtc gtgatattag aaaagaactg
      481 gcctatgtga ttaatggcag gttcctgaga aaagatatta agaaagataa aatcacggac
      541 cgggaaatgg aaattatccg catgacggcc cagggaatgc aacctaaatc gattgccaga
      601 attgaaaatt gtagtgtgaa gacagtgtat acccatcggc gtaatgctga ggccaagctg
      661 tactcaaaaa tatataagtt ggttcagtaa actccaggca agttagtttt aaaaaatgac
      721 tcactgggac atcacgtcct caattcaact cgggaagaaa tgcaatgaaa aaaaaggttc
      781 tggcaatagc tctggtaacg gtgtttaccg gcatgggtgt ggcgcaggct gctgacgtaa
      841 cagctcaggc tgtagcgacc tggtcggcaa cagccaaaaa agacaccacc agtaagctgg
      901 ttgtgacgcc actcggtagc ctggcgttcc agtatgccga aggcattaaa ggttttaact
      961 cacagaaagg tctatttgac gtggctatcg agggtgactc aacggctacc gcctttaaac
     1021 tgacctcacg tcttatcacc aacacattaa cccagttgga tacctcaggt tccacactga
     1081 atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac tggcgatacc gtgatgatcg
     1141 ataccgccaa cggcgtactg ggcggcaacc ttagcccgct ggctaacggt tacaatgcca
     1201 gcaatcgtac caccgcacag gatggtttca ccttctccat catcagcggt accaccaatg
     1261 gtaccaccgc agtaacagat tacagcactc taccggaagg catctggagc ggcgacgtta
     1321 gcgtacagtt cgacgcgacc tggaccagtt aatctctctg atgtaccagc aggggtagcc
     1381 cccctgcttt attcctggac ggaattgtta tgaaaaagca ccttctgcct ctcgctctgc
     1441 tgttttccgg aatatctccg gcccaggcgc tggatgtcgg cgatatatca tcgtttatga
     1501 acagtgacag cagcacgctg agcaaaacga tcaaaaacag taccgacagt ggtcgcctta
     1561 tcaatatccg tctcgaacgg ctctcttcac cgcttgacga cgggcaggtt atctcaatgg
     1621 acaagccgga tgagttgcta ctcactcccg ccagcttgct gctacccgcc caagccagcg
     1681 aagtgatccg cttcttctat aagggacccg cagatgaaaa agagcgctac taccgcattg
     1741 tctggtttga tcaggccctc agtgatgcgc agcgcgataa tgccaaccgc agcgctgtgg
     1801 ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc ccctcgtcag gcgaactacc
     1861 actttcagta cgccaacggc tccctgacaa atacaggaaa tgcgacgctg cggatcctcg
     1921 cttacggccc ctgcctgaaa gccgccaatg gtaaggagtg taaagagaat tactacctga
     1981 tgccaggcaa gtcgcgtcgt tttacccgcg tggacacggc ggataacaaa ggacgggttg
     2041 cactttggca gggtgataag ttcattcccg tgaaatagat agctgtgcag atggataacg
     2101 acaatgcctt tacgacggtt ctccccagga ctgaaagccc agtttgcctt cggcatggtc
     2161 tttttgttcg ttcaacccga tgccagcgct gctgacataa gtgcgcagca aataggtggg
     2221 gtgattattc cgcaggcctt cagtcaggcg cttcaggacg gcatgagcgt cccgctctat
     2281 attcatctcg ccggtagcca gggtcgccag gacgatcagc gaatcggcag cgcttttatc
     2341 tggctggatg atggacagct acgcatccgg aaaatacagc tggaagagag tgaagataac
     2401 gccagtgtca gcgaacaaac tcgacagcag ctgatggctc tggcgaacgc cccgttcaat
     2461 gaggccctta ccatccccct gactgacaac gcgcagctgg atctcagctt gcgccaactg
     2521 ctgctgcagc tggtggtcaa gcgcgaagcg ctgggcaccg tactacgctc acgtagcgaa
     2581 gacatcgggc agtccagtgt taacaccctc agcagtaatc tgagctataa cttgggcgtc
     2641 tataacaacc agttgcgtaa cggcgggagc aacacatcca gctatctgtc gctgaataac
     2701 gttactgcgc tgcgcgaaca tcatgtggtg ctcgacggct cgctgtacgg gatcggtagc
     2761 ggtcaacagg acagtgaatt atataaagcg atgtatgaac gcgattttgc cggtcaccga
     2821 tttgccggtg gaatgctcga cacctggaac ttgcagtcct tagggccgat gaccgccatt
     2881 tcagcaggga agatttacgg cctttcctgg ggaaaccagg ccagctccac catcttcgac
     2941 agcagccagt cagccacgcc agtgatcgcc tttttaccgg cggcgggcga agtacatctc
     3001 acccgtgatg ggcggctact aagcgttcag aacttcacta tgggcaatca tgaagtggat
     3061 acccggggtc taccgtacgg gatttacgat gtggaagttg aggtgatcgt taacggtcgc
     3121 gtgatcagca aacgcaccca gcgggtcaat aagctgttta gccgggggcg cggcgtcggt
     3181 gcaccactgg cgtggcaggt atggggcggt agctttcata tggatcgctg gtcggaaaac
     3241 gggaaaaaga cgcgaccagc taaagagagt tggctagcag gtgcctcgac ctccggctca
     3301 ttgagtacgc ttagctgggc ggcaacggga tatggatacg ataatcaggc ggtgggtgaa
     3361 acccgtctga cgctgccgct tgggggggcg atcaacgtta acctgcaaaa tatgctggcc
     3421 agtgacagct catggagcag catcggcagc atcagcgcca ctctaccggg aggctttagt
     3481 tcgctgtggg ttaaccagga aaaaacccgc attggcaatc aattgcgacg tagcgatgcc
     3541 gacaaccgtg caatcggcgg cacactcaac ctgaactcac tgtggtcgaa gctgggcaca
     3601 ttcagcatca gctacaatga tgaccgccgt tacaacagcc attattacac ggcagattac
     3661 tatcaaaata tctacagcgg tacctttggt tcgcttggcc tgcgggccgg tattcagcgc
     3721 tataacaacg gcgacagcaa cgccaataca gggaaatata tcgctctcga tctctcgcta
     3781 ccactgggca actggtttag cgcagggatg actcatcaaa acggctacac catggcaaac
     3841 ctgtcagcac gcaaacagtt tgatgaagga accattcgca ctgttggtgc caatctgtca
     3901 cgtgccatct ccggcgatac cggtgatgac aaaaccctca gcggtggggc gtatgcacaa
     3961 ttcgacgctc gttacgccag cggaacgctg aacgtcaata gcgcggcgga cggctacgtc
     4021 aataccaact tgaccgccaa tggcagcgtc ggctggcagg gtaaaaacat tgctgccagc
     4081 gggcggactg atggcaacgc tggggtgata ttcaacaccg ggctggagga cgacggtcag
     4141 atcagcgcca aaatcaacgg gcggattttc ccgcttaacg gcaagcgtaa ctatctcccg
     4201 ctctctccct atggaagata tgaggtggag ttacagaaca gcaaaaactc actcgacagc
     4261 tacgatatcg tcagcggccg caaaagccat ctgactctct atccaggcaa tgtcgctgtc
     4321 attgagccag aggtgaagca gatggttacc gtctccggtc gtatccgtgc ggaagacggc
     4381 acactgctgg ctaacgcacg gattaacaac catatcggcc gaacccgaac cgatgaaaac
     4441 ggcgagtttg tcatggacgt ggataagaaa tatcccacta tcgattttcg ctacagtggc
     4501 aataaaacct gcgaagtggc actggaactc aaccaggcgc gcggtgccgt ctgggtcggt
     4561 gatgtggtct gcagcggcct ctcatcgtgg gcggcggtga cgcagacagg agaagagaat
     4621 gagagttaac ctactaataa agatgataat tttcgcgcta atctggccag taactgagct
     4681 cagagcggca gtgagcaaaa caacctgggc ggatgcaccg gcacgcgagt ttgtgtttgt
     4741 cgaaaacaac tcagacgaca actttttcgt cactcctggc ggggcgctgg atccgcgcct
     4801 gaccggtgcc aaccgctgga ccggtttaaa atacactggt tcaggaacca tctatcagca
     4861 aagcctcggc tacattgata acggttacaa caccggcctt tataccaact ggaagtttga
     4921 tatgtggctg gaaaattcac cagtttcatc tcctttaact ggcttgcgct gcatcaactg
     4981 gtacgctggg tgtaatatga ccaccagtct tatcctgccg caaaccaccg acgccagtgg
     5041 attttatggc gcgacggtga ccagcggcgg cgcgaagtgg atgcacggca tgttgtcaga
     5101 cgcgttttac cagtatctgc aacaaatgcc cgtcggcagc agctttacaa tgaccatcaa
     5161 tgcctgccag acctctgtga actatgacgc cagcagcggc gcacgctgta aggatcaggc
     5221 ctccggcaac tggtatgttc gcaacgtcac ccatacgaaa gcagcaaatc tgcggttaat
     5281 aaatacccac tcgctggcgg aggtatttat caacagcgac ggagtaccga ctctgggcga
     5341 agggaacgcc gactgccgga cacagaccat cggcagcctt tcaggattaa gttgtaagat
     5401 ggtcaactat accctgcaaa caaacggact cagcaacacc tcaatccata tattcccggc
     5461 gatcgccaac tcgtcgttag cctcggccgt cggggcgtac gatatgcagt tcagtctgaa
     5521 tggcagttca tggaaaccgg tgagcaatac cgcctattac tacaccttca acgagatgaa
     5581 gagcgcagac tcgatctatg ttttcttctc gagcaacttc tttaagcaga tggtgaacct
     5641 cgggatcagc gatatcaaca ccaaagatct attcaacttt cgctttcaga acaccacatc
     5701 accggagtct ggctggtatg aattttctac ctccaacacg ctgattatca aaccccgtga
     5761 tttcagcatc agtattatct ccgatgaata tactcagaca ccgtcgcggg agggatatgt
     5821 tggcagcggc gagtcggcac tcgatttcgg ctatatcgta accaccagcg gtaaaacagc
     5881 tgccgacgaa gtgctgatca aggtgaccgg acccgcgcag gtgattggcg ggcgctccta
     5941 ttgtgtcttc agctccgatg acggtaaggc gaaagtaccg ttcccggcga cactttcctt
     6001 tattacccgc aacggagcta caaaaaccta cgatgccggg tgcgatgata gctggcggga
     6061 tatgaccgat gcgctgtggt tgaccacacc gtggactgat atctctggcg aagtggggca
     6121 gatggataag accacagtca aattttcgat tccaatggat aacgccattt cgctgcgtac
     6181 ggtagatgat aacggctggt ttggcgaagt cagcgcttca ggagagattc atgttcaggc
     6241 gacgtggcgt aacattaact aaggccctgc tgacagcggt ctgtatgctg gcggcacctt
     6301 tgacacaggc gatttcggtc ggcaatctga cattttcgct gccgtccgag actgactttg
     6361 tcagcaaacg tgtagtgaat aacaacaaaa gcgcacggat ataccgtatt gccatcagtg
     6421 ctattgatag cccgggcagc agtgaattgc gcacccgacc ggtggatggt gaactgcttt
     6481 tcgccccccg ccagctggcg ttgcaggctg gtgagagcga gtattttaaa ttttactatc
     6541 atggtccacg ggataaccgc gagcgctact accgggtctc atttcgcgag gtccccactc
     6601 gtaaccagac aagacgcagc ccaaccggcg gcgaggtcag cacggagccg gtggtggtga
     6661 tggataccat tctggtagta cgaccacgtc aggttcagtt taaatggtcc ttcgacaagg
     6721 tgacaggaac ggtaagtaac actggcaaca catggtttaa gctactgatt aagccaggat
     6781 gtgattcgac cgaagaggaa ggcgatgcct ggtatctacg tccgggagac gtggttcatc
     6841 agcctgagtt acgtcagccg gggaatcatt atctggtcta taacgacaaa ttcattaaga
     6901 ttagcgattc ctgtccggct aagccccctt cggcggacta agacttatcc atcggtcgag
     6961 ggaagaattc cacctcagag ctccaaaaat cgtttaaatg atgtggaagc gatcgtcaat
     7021 gtcacctgta acgatgggct ttctcatatg cacaccatag tgttcaggag acacggaaag
     7081 gtatcaggca gcctgctaac gagtggttta atcacgaatt agtacgtaaa atcggtaacg
     7141 gctggaaatc attcaatact cacactatcg aaagttcccc agccaaccgc ggtacgttct
     7201 tacatacgat gtaccgctgt tctctttacg atttttagct gtactggtga attatgagca
     7261 atctgaatcc atgcatgacg tgtggtgcct gttgtgcatt tttccgcgtc tctttttact
     7321 gggccgaagc cgacgatgct ggcggtacta ttcccgccag gctcactgaa caaatatccc
     7381 cttttcaccg atgcatgagc ggtaccaatc agaaaaaccc ccgatgtatt gcccttgcag
     7441 gaaccccggg caaaaatgcc tgctgcacga tatataaaaa tcgatcgtcc acatgcagag
     7501 aatttgccat gtctggtgag aacggagtcg tcaatgaggc ttgcaatcgt gcaagggcta
     7561 aatacgggct gacaccgtta taaacataca ataattaatt gcactgcccc gccagcgata
     7621 atagcggggc ttcgtttttc aggggtaaca aaacccgata cttcttctat ttgccagcaa
     7681 caatgcctct cttctgtagc gttcttcgga tctacatcat cctgaag
//
LOCUS       DAZFAH010000199         7698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFAH010000199 DAZFAH010000000
VERSION     DAZFAH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.201"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..105)
                     /locus_tag="ACHJWB_004053"
     tRNA            complement(29..105)
                     /locus_tag="ACHJWB_004053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Arg,seq:acg)
     gene            complement(303..379)
                     /locus_tag="ACHJWB_004054"
     tRNA            complement(303..379)
                     /locus_tag="ACHJWB_004054"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Arg,seq:acg)
     gene            complement(383..475)
                     /locus_tag="ACHJWB_004055"
     tRNA            complement(383..475)
                     /locus_tag="ACHJWB_004055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Ser,seq:gct)
     gene            complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHJWB_004056"
     CDS             complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHJWB_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4315595.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHJWB_004057"
     CDS             complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHJWB_004057"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4315596.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAVLASVKGWVSAKLQ"
     gene            complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHJWB_004058"
     CDS             complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHJWB_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4315597.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHJWB_004059"
     CDS             complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHJWB_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4315598.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHJWB_004060"
     CDS             complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHJWB_004060"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4315599.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHJWB_004061"
     CDS             complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHJWB_004061"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4315600.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1794 a         2164 c         1859 g         1881 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattcga acctccgacc
       61 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaacttact
      121 ttactgcaga ttttttgata ccgctactaa agccgtatca agtaagagat ggtgcatccg
      181 ggaggattac tcgccttgcg gctcgccctt tgggccgttg ctaaagcaac gttatcctcc
      241 ctggtgcctg cggttatacc gccacctctt ccataccttc atcgctgaaa gtacgtagaa
      301 tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat
      361 ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt
      421 ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg
      481 cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata
      541 ttactttctg agacttataa gtcaaacaat ttttcctaca cttttatcgt ttgcacactt
      601 cacgttcaat tagtctgtaa aaacgtcaaa aagagtgttt tatcaacaga aaaatggagg
      661 tctgacagat agtagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag
      721 cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga
      781 cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct
      841 cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct
      901 ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac
      961 gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc
     1021 ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag
     1081 cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg
     1141 actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct
     1201 gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct aacactgcag
     1261 gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc
     1321 cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt
     1381 ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa
     1441 ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa
     1501 caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa
     1561 gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact
     1621 gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca
     1681 gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt
     1741 ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc
     1801 ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga
     1861 aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg
     1921 cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac
     1981 gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt
     2041 tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat
     2101 gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac
     2161 gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag
     2221 aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca
     2281 ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt
     2341 ccgcatagaa tggtgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat
     2401 caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat
     2461 agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc
     2521 cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt
     2581 caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg
     2641 tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct
     2701 cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct
     2761 tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa
     2821 cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc
     2881 cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg
     2941 gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca
     3001 gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc
     3061 ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca
     3121 gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc
     3181 ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg
     3241 gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg
     3301 ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt
     3361 tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt
     3421 cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa
     3481 accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt
     3541 cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga
     3601 cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc
     3661 gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt
     3721 tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat
     3781 gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca
     3841 taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc
     3901 cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc
     3961 gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca
     4021 cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca
     4081 ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc
     4141 atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt
     4201 gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca
     4261 tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca
     4321 tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct
     4381 tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat
     4441 gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag
     4501 ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag
     4561 aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
     4621 gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
     4681 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc
     4741 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4801 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
     4861 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4921 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
     4981 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
     5041 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     5101 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     5161 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     5221 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     5281 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5341 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
     5401 gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
     5461 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
     5521 gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga
     5581 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5641 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5701 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5761 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5821 ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5881 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag
     5941 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
     6001 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
     6061 tcagcacgag ccgctttcag tgcgcctatc accatttcca ccacgacggg ttcactcacc
     6121 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
     6181 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     6241 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
     6301 agcgcctgcc caacctgttc acttaactgc atcagttcac tgtcagtcat cacaacctct
     6361 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6421 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6481 cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6541 gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg
     6601 taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg
     6661 cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct gtacctggtt
     6721 ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcaac ctgatcgcct
     6781 ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc
     6841 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6901 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6961 tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
     7021 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     7081 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
     7141 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     7201 acgttgtccg gcgtaataaa ttttttgcga taacggagcc atgcgccgtt cggacctgat
     7261 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7321 cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7381 attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
     7441 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7501 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7561 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7621 taaagcatca cgccccacac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
     7681 ctcaactcat tcccctcg
//
LOCUS       DAZFAH010000200         7657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFAH010000200 DAZFAH010000000
VERSION     DAZFAH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.202"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..169)
                     /locus_tag="ACHJWB_004062"
     CDS             complement(<1..169)
                     /locus_tag="ACHJWB_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4315601.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKEN"
     gene            382..1173
                     /locus_tag="ACHJWB_004063"
     CDS             382..1173
                     /locus_tag="ACHJWB_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO4315602.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1183..2025
                     /locus_tag="ACHJWB_004064"
     CDS             1183..2025
                     /locus_tag="ACHJWB_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4315603.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AYYC"
     gene            2035..2811
                     /locus_tag="ACHJWB_004065"
     CDS             2035..2811
                     /locus_tag="ACHJWB_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO4315604.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTI
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2821..4362
                     /locus_tag="ACHJWB_004066"
     CDS             2821..4362
                     /locus_tag="ACHJWB_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase subunit
                     alpha"
                     /protein_id="HFO4315605.1"
                     /translation="MRKITTAGALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVVQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4359..6440
                     /locus_tag="ACHJWB_004067"
     CDS             4359..6440
                     /locus_tag="ACHJWB_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO4315606.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQHLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6461..6937
                     /locus_tag="ACHJWB_004068"
     CDS             6461..6937
                     /locus_tag="ACHJWB_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO4315607.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            6980..>7657
                     /locus_tag="ACHJWB_004069"
     CDS             6980..>7657
                     /locus_tag="ACHJWB_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFO4315608.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPS"
BASE COUNT         1834 a         1879 c         2015 g         1929 t
ORIGIN      
        1 aattttcttt attaccaaag tgatgataaa ccgtctgttt actacacccc gcagccttaa
       61 caatattatc gaccctggcc ccttcataac catattcggc aaattcatta accgcgcata
      121 acagcagctt ttccttcagg ctctgtacgt tgctggtatc aagatacata acgcttcccc
      181 acgacctctt caagataact atcttaaccc gattaattag aagctccagt aaaaaggata
      241 aaaacttgta ctttcatcaa gttatcaaat gtcctgtgat cactatcgca tatcaaccaa
      301 ccagatggtt gatctttctt gcggcacgtc gcaagctaat tgtcacttag taaacaatat
      361 tcaaaaaaag agagctattg tatgaaatcc actcgtcctg ttgccgtgat taccggagcc
      421 gctcgtggta ttggaaaggg ctgtgcgctg gagcttgctc gtggcggatt taacctgctg
      481 attaacgatc ttcccgatgc cgacagcgta gaaaaactgc atatcacaca acaagaatgt
      541 atagctgaag gcgtggaagt gatttgcttc cctgccgatg tcggcgatct ctccttgcat
      601 gaagagatgc tcgacgcggc gcaaaacctg tggggacgtc tggactgcct gctcaacaac
      661 gcaggcattt cagtgaaaaa acgcggtgac ctcctcgacc tcgaaccaga cagcttcgat
      721 caaaacattg ccattaacac ccgcgcgccg ttcttccttg ctcaggcatt cagtaagcgc
      781 ttactggcac agccgaaacc ggaagcggag ctgccgcacc gctcgattat tttcgtcagt
      841 tccatcaacg ccatcatgct ggcgatgaat cgcggcgaat acaccattgc caaaaccgcc
      901 gtttctgccg ctgctcgtct gtttgctgct cgcctttgta atgaacaaat tggcgtttat
      961 gaagtgcgcc ctggtctcat caaaactgat atgactattc cggcaacggc ttattacgac
     1021 gaactgattg ccaaaggtct ggtgccgtgg ggacgttggg gttatccggc tgatattgct
     1081 tccaccgtcc gcgcgatggc ggaaggtaaa ctgatttaca cctgtggtca ggcggtcgcc
     1141 atcgacggcg gcttaagtat gccgcgcttc tgaggctgaa tcatgagaga tctgttacaa
     1201 cgcccggatt tattcagtat caataccgcg acactgggct ataaaacgcc gctacccgcc
     1261 attattgatg cctgcgccgc acgcggtatt ggcgcaattg ccccctggcg cagggagttg
     1321 cagggtgaag atctccagca aatcacccgc cagcttgccg ccagtaacat gagcgtttcc
     1381 ggtttgtgcc gtagcactta ctacaccgcg ccgacactgg cggaacgcaa actggcgatt
     1441 gatgataacc gccgggcgct ggacgacgca gcggtgctta acgccgcctg ttatatgcag
     1501 gtggttggcg gtctgccaac gggcactaaa gatctatatg aagcgcgaga acaagtgaaa
     1561 caagggattc gccagttgct gccacattcg aaagatgtcg gcgtgccaat tgcgctggag
     1621 ccactgcacc cgatgacagc tgccgatcgc tcttgcctgt gcacgttacg tcaggcgctg
     1681 gactggtgtg atgaactcga tcccgatggt gagtttggcc tcggcgtggc ggtggatgtt
     1741 tatcacgtct ggtgggatcc ggatctcgcc agtcagatcc tgcgtgccgg aaaacgcatc
     1801 ctcgcgtttc atgtttccga ctggttagtg ccgaccaccg atctggtcaa tgaccgggga
     1861 atgccgggag atggtgtcat caatattccg tcaattcgtc ggctggttga aaacgccgga
     1921 tttaatggcg ctatagaact ggaaatattc tcgccttact ggtggcagaa agatattaac
     1981 agcacgctgg atattagcgt cgatcgcatc gcgtattatt gttaaggaat gatcatgagt
     2041 gaccaacccg ttctgttcag cagagcggca gcttcctgcc gcctgacatt gaaccgtgaa
     2101 gataaatgtc atgctattaa cgaagagatg atcgaatctc tcgaccatta tttaaatgaa
     2161 attgaaaacg acaccacatt acgattagtc gaattaacgg caaccggcga taaattcttt
     2221 tgtgcgggcg gcgatattaa atcgtggtca gcctattcgc cattagatat gggcagaaaa
     2281 tggattaaac gcggtaatga cgtttttaat cgcctgcgaa acttacccca attaaccatt
     2341 gcgaatctca acggtcatac catcggcggt ggtattgaac tggccttgtg ctgcgatatt
     2401 cgtattgccc gccccggcgc gaaattctct aacccggaag tgatgctcgg catggtgcct
     2461 ggctggatgg gcattgaacg tgtgcttaat caggttggtc cggtggtcgg tcgccagatg
     2521 ctgatgctgg gtaaacgcct gacggcgcag gaagcccagg cggcgaattt aattgatgaa
     2581 gtggtagaaa aagaacaggt ggaaagctgg atggcgaacc agttagcgca gctggaaaaa
     2641 tgcggtccgg tggcactggc gcatattaag caactgattc tggcactgga aaataaacat
     2701 gccgattacc cgcaccagct actggccgga ttaatgtctg ccactcagga ttgccagcag
     2761 gcgacgcgcg cctttgccga aaaaagtagc gtgagcttcc ataatcaata aggaaattgt
     2821 atgcgtaaga taacaaccgc cggggcactg gcggcgcaaa ttcaggacgg tgcgactatt
     2881 gctattagcg gtaacggcgg cggtatggta gaagccgacc atattctggc tgctattgaa
     2941 gcccgtttcc tgcaaaccgg acaccctcgc gacctgacgc taatccactc gctgggtatt
     3001 ggcgatcgcg actgcaaagg cactaaccgt tttgcgcacg cagagatgct caaacgcatt
     3061 attgccgggc actttacctg gtcgcccaaa atgcaggcgc tggtaaaaaa taacacgatt
     3121 gaagcctact gctttcctgg tggcgtggtt caggcactgc tacgggagat cggcgccgga
     3181 cgtccggggc tttttaccca cgttgggctg ggatcgtttg ttgatccacg caatggcggc
     3241 ggtaagtcga atgaatgcac taccgacgac ctggtagaac tgattgaaat cgatggtgaa
     3301 accaaacttc gttatcgccc tttcaaagtg gattacgcga ttttgcgtgg cacttatgcc
     3361 gatcctcgag gcaacgtcag ccttgaagaa gaagcgattg atatggatag ctattccatg
     3421 gcgctggcag cacacaacag cggcggcaaa gtgttcgtac aggtacgcga tgtgctggaa
     3481 gcaggtgcca ttgaaccacg tcgggtcaag ctaccaggaa ttctggtcga tggcatcgtt
     3541 gagcaccgcg aacaaccgca aacatatctt ggtggttacg acctgaccat tagcggccaa
     3601 catcgccgtc tgagttctaa cgacgctatt gaactggtta gtcatccggt gcgccgcctg
     3661 attgcccgtc gggcagcacg cgaactggtg gctggcgctt caaccaactt tggctttggt
     3721 attcctggcg gtattccagg agtagcgctg cgcgaaggcg ttccttatca aagtttatgg
     3781 ctgagcgtcg agcaaggcgt gcataacggc atgatgctgg atgatgctct gttcggctgc
     3841 gcccgcaacg ccgatgccat tattccatca ctcgatcaat tcgaattcta cagtggcgga
     3901 gggatcgata tcaccttcct cggcatggga gagatggatc agtacggtaa cgtcaacgtc
     3961 tcccatctca atggcaatct gattggcccc ggcggatttc tcgaaattgc gcaaaacgcc
     4021 cgtaaagtgg tgttctgcgg cacgttcgac gccaaaggca gcaagattga tgtaacacca
     4081 gatggcttgc atatcgccca gtcaggtcaa atccctaaac tggttaccca ggtggaaaaa
     4141 atcactttta gcgccgccta cgcacagcaa agtggtcagg aagtgttgta tatcactgaa
     4201 cgcgcggtgt ttcagttaac ggcagaaggc gttgaattaa ttgaaatcgc accaggtgtg
     4261 gagattgagc gcgacattct gccgtatatg gctttccgtc caattatcaa gcacccacgc
     4321 ctgatggaaa gtagcctgtt tacgccgatg gaggatgcat gagtcagctt gatgacacca
     4381 tccttgatgc gctgacgcac gttactttcc caaagggttt tgcacaggca gagcccgcat
     4441 gggttgtcac ggtagacggt gttgattacc cactctggca aacagatgcc ctggtagtcg
     4501 gcagtggtgc agccgggctc cgtgcagctg tggaacttaa acgccgccag caaaatgtgc
     4561 tgatcgccac cgccgggtta tatatgggga cgtcggcatg ttctggttcc gataaacaga
     4621 cactgtttac cgccgctacc gcgggcaacg gcgacaactt caccaaactg gcagaggcac
     4681 tggcgagcgg tggggcgatg gatcacgaca ccgcttatgt cgaagcggta ggttctctgc
     4741 acactcttgg cgggttgcaa tatcttggtc tggaattacc ggaagatcgc tatggcgcga
     4801 ttcttcgtta tcaaaccgac catgacgaag ccgggcgtgc aacctcgtgt gggccgcgta
     4861 cctcaaggtt aatggtgaaa gtgctgttgg aagaagtaca gcacctcgcc attccagtgt
     4921 tgaccagtgc aacagtgatt aaactgctgc atcagcgtga cgaaaacggc gaagaccgcg
     4981 tggcaggggc aatcctcgcg accggtcatc gcgcccataa cccatggggg ctggcaattg
     5041 tgactgcgcc caatgtggta ctggcaacgg gcggaccggg cgagctttat cgcgacagtg
     5101 tttatccaca taaatgcttc ggctcgctgg ggctggcgct ggaggaaggt ctgacgctaa
     5161 ccaatctgac cgaaagccag tttggtatag gcacgccgcg tagcacgttt ccgtggaatt
     5221 tatccggcac ctatgtacag gtgatcccgt atatctattc cgtggatgct gagggtaacg
     5281 agtataactt cctcgcggat tactatcgca ccacccagga gctggcttca aacattttcc
     5341 gtaagggcta tcagtggccg ttccacgcca ctcgggtgat ggattttggc tccagcttgt
     5401 tagatatggc agtagcgcaa gagcagcaat cagggcgtca ggtatttatg gatttcaatc
     5461 gcaatcctga agcggtgccg ggtgacctgc cgttctcatt agatcgactg gacgacgacg
     5521 ttcgcgcgta tctggaaaat aacgacgctc tggcaccatc gcccatcgaa cggctgcaac
     5581 gaatgaatcc gctgtctatc tcgctgtata agatgcacgg ttacgatctc accacacagc
     5641 ctttgcagtt tgccatgaat aatcagcata tgaatggcgg cattgaagtg gatatctggg
     5701 gacaaacctc cctgcccggc tgtttcgccg tgggggaagt tgctggcaca cacggcgtca
     5761 ctcgccctgg tggtgcagca ttgaatgccg ggcaggtttt tgctgttcgt ctggcacgtt
     5821 ttattggttg cacgcaaaag cggaatattg atggagatat agcacagctg gtagctccta
     5881 cactggcttc tataagagag atcattactc aggcgcacga taacgggacc gggatgccgt
     5941 tgtcggttgt gagagaaaaa attcaggcac gaatgtctga ccatgcggga tttatttgcc
     6001 atgccgataa agtccgacgc gccactcgcg atgccctgct attgagcgaa tttgtccaac
     6061 ggcatggatt ggctatcaaa catgtgggtg aagttgccga gctgtttatg tggcggcata
     6121 tggcactgac ttctgccgcc gtattaacgc aattaacgca ttatattgac gcaggtggtg
     6181 gtagtcgggg agcacgtatt attctcgaca gggatggaaa ttctattccg caaacacgaa
     6241 atggtttttg tgatgcctgg cgctttcgtt cagagcgtac tgaggataaa aaagataaac
     6301 tacttattca ttattgcaac ggtatctttc acgtcagaga aacacctgtt cgtgagtttc
     6361 ctattatccg aggtatttgg tttgaaaaaa actggcctgg ttttcttaat gggacaattt
     6421 accagccaca ggatgaatga tattgcaaag gttaatcact atgcgaaata taattagatc
     6481 tattaatgct ctgcttgcag cattgaacat taccattttg gcgatcatcg tggcatgtgt
     6541 gacgtggcag gtggctgcac gatttatatt tactagtccc agtattttta cagatgaact
     6601 cagccgtttg ctctttattt gtcttggact ttttggtggg gcatacaccg cagggcaaaa
     6661 ccggcatctg gcgattgacc tgctacctat gatgctgaaa ggaaaagccc gacgtcattt
     6721 atttttgtgt attcagatta tcgttattat cttcgcaacc atcattatgg tatatggtgg
     6781 tgggctatta acgatggata cattcgattc aggtcagacc tcgcccgctt taggctggca
     6841 aatgggatat atctatatga gcatcccgat tagtggtgta cttattatta tatacaccat
     6901 agatatggtt ttaaccgaac ttaaacaacc actctaaaga taacaaacaa caaccaacca
     6961 tagtaggaat ggagaaatta tggattactg gttaccgatt attgtgttat ttggtgcctt
     7021 cttttttatg ttggcattag gtgtacctat tgtttatgcc attggtttat cgacactggc
     7081 atcaatttcc acacaactcg atttcaactc agcattgagt gtggtttccc aaaagttagc
     7141 ctccgggtta gatagtttta cgttgttggc aatcccattt tttatcttgt ctggcaacat
     7201 tatgaaccat ggaggaatag cgcgacgatt aatcaacttt gcccgtattc tcggcggtcg
     7261 tctgccaggc tcacttgccc actgtaatat tctcgccaat atgctgttcg gggcaatttc
     7321 tggttcagcc gtggcttcgg cggctgcgat gggcggcgtc atgcatccgc aacaggttaa
     7381 agagggttac gacccggcat tcagtactgc ggttaacgtt gcctctgccc cgacgggttt
     7441 gcttattcca ccaagtaata cgttaatcgt ctattcactg gtcagtggtg gtacttctat
     7501 tgccgcactg ttcctggcgg gttatgttcc gggaattttg ctgggtctgg cactgatggt
     7561 aatagccggt attatcgcgg tacgtcgcgg ttatccaaag ccagaacgcc cgactttacg
     7621 ccaggctggc gttgcgatat ggatggctat ccccagt
//
LOCUS       DAZFAH010000201         7538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFAH010000201 DAZFAH010000000
VERSION     DAZFAH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.203"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..245
                     /gene="yafQ"
                     /locus_tag="ACHJWB_004070"
     CDS             <1..245
                     /gene="yafQ"
                     /locus_tag="ACHJWB_004070"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO4315609.1"
                     /translation="SKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSW
                     KGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            401..1141
                     /gene="dpaA"
                     /locus_tag="ACHJWB_004071"
     CDS             401..1141
                     /gene="dpaA"
                     /locus_tag="ACHJWB_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4315610.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1112..1879)
                     /locus_tag="ACHJWB_004072"
     CDS             complement(1112..1879)
                     /locus_tag="ACHJWB_004072"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4315611.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKLLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHJWB_004073"
     CDS             complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHJWB_004073"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4315612.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2903..5347
                     /gene="fadE"
                     /locus_tag="ACHJWB_004074"
     CDS             2903..5347
                     /gene="fadE"
                     /locus_tag="ACHJWB_004074"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4315613.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHJWB_004075"
     CDS             complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHJWB_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4315614.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTIRSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCCSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6017..6784
                     /gene="yafV"
                     /locus_tag="ACHJWB_004076"
     CDS             6017..6784
                     /gene="yafV"
                     /locus_tag="ACHJWB_004076"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4315615.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7008..>7538)
                     /locus_tag="ACHJWB_004077"
     CDS             complement(7008..>7538)
                     /locus_tag="ACHJWB_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4315616.1"
                     /translation="NKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVLDE
                     LIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWH
                     VENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAA
                     MDRNYLASVLAGCGLS"
BASE COUNT         1783 a         1901 c         1971 g         1883 t
ORIGIN      
        1 tttcaaagga tgtaaaactt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc
       61 ttatgacgct tcttatcaat aatgctttac cgcttccagc tgtttataaa gaccacccgc
      121 tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt
      181 acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg
      241 ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc
      301 ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat ccattttgcc
      361 gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat
      421 tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggc ttgctgggca gcagtagctc
      481 gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca
      541 gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcagct
      601 gctcgacagt tataaaatct gtaaatattc cggcggatta gggccaaaac agcgtcaggg
      661 tgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag
      721 ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta
      781 tgaagggaaa tatctgatga tccacggcga ttgtgtttcc atcggctgct acgcaatgac
      841 caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc
      901 gagcgtgcaa gtgagtattt acccgttccg catgaccgac gccaatatga agcgccataa
      961 gtattccaac tttaaggact tctgggagca actgaaaccg ggctacgact actttgagca
     1021 aacccgtaag ccaccgaccg tttctgtggt caatggccgt tacgtggtca gcaagccgtt
     1081 aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata
     1141 agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg
     1201 caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct
     1261 gatccagcaa cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg
     1321 agcaatacgc cattacatag cgcccgtcag aaagcagcat attgaaaacg cccttctgcc
     1381 gcagttcatc cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtac
     1441 gcgggtaacg ctgcgttaat ttatgcagca gccagcagaa ggctttttcg ctgtcggttt
     1501 cgcctaccgg gcggaagttg ccggtttcca gtaatttgta gcccgtcagt tgtccgttat
     1561 gggcataagt ccagttacgc ccccataact cgcgggtaaa tgggtgagta ttttccagcg
     1621 ccacctcgcc ccgattagcc tggcgaatat gagccaccac cgaacaggat tttatcgggt
     1681 agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct ttaaatgtgc
     1741 gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac
     1801 caccacgctg tacaagtccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca
     1861 tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga
     1921 agattgattt aagaattttc aggccggatg cggcgtaaac gaattattcg gcctacggtt
     1981 cgcgtacaga tttgtaggca tgataagacg cgtcagcatc gcatcaggca tcagcgcaca
     2041 agtgccggat gcggcgtaaa cgccttatcc ggcctacgcc agacttactt aaccatctct
     2101 ttttcaatta actggatcag gatatggatc actttaatgt gaatctcctg aatgcggtcg
     2161 gcataaccaa agtgcggtac gcgaatttcg atatccgccg tgccagccat tttgccgccg
     2221 tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgctgc gatcgctttg
     2281 atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc ttcgcgacct
     2341 accgcttcaa cgtagcggga gaaaatatca ttgaaaccga aatcattacc gacgcaggaa
     2401 atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc acggtagcga
     2461 ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa
     2521 agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg ctgaatggcg
     2581 tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc gttcagttcg
     2641 ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat agacaaaatg
     2701 cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac caggactttt
     2761 tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt atattgtaaa
     2821 cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg tcagacctcc
     2881 tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct
     2941 cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt tgctcgcctg
     3001 gacagccgcc ctcggcgttg ctggtctgtg gtcggcgtgg gtactggtgc ctctggccat
     3061 tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt
     3121 tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag cgattgatgc
     3181 gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga aaaagctgca
     3241 taactatccg cagccgcgcc tgaccgccga agagcaagcg tttctcgacg gcccggtaga
     3301 agaagcctgc cggatggcga atgatttcca gatcacccat gagctggcgg atctgccgcc
     3361 ggagttgtgg gcgtacctta aagagcatcg tttcttcgcg atgatcatca aaaaagagta
     3421 cggcgggctg gagttctcgg cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt
     3481 gagcgggatc ctggcgatta ccgtcggcgt gccaaactca ttaggcccgg gcgaactgtt
     3541 gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca
     3601 ggagatcccc tgctttgcac tgaccagccc ggaagcgggt tccgatgcgg gcgcgattcc
     3661 ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg ggatgcgtct
     3721 gacctggaac aaacgctaca ttacgctggc accgattgcg actgtgcttg ggctggcgtt
     3781 taaactctcc gacccggaaa agctgctggg tggcgcagaa gatttaggta ttacctgtgc
     3841 gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt
     3901 accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg attacattat
     3961 cggcggacca aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg
     4021 ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag cgctggcaac
     4081 gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga tggaagggat
     4141 tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg cggcatcgct
     4201 gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta
     4261 tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca ctggtggcaa
     4321 aggcattatg ctcgggcaga gcaacttcct ggcgcgtgct taccagggcg caccgattgc
     4381 catcaccgtt gaaggggcca acattctgac ccgcagcatg atgatcttcg gacaaggagc
     4441 gattcgttgc catccgtacg tgctggaaga gatggaagca gcgaagaaca atgacgtcaa
     4501 cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag
     4561 cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg atgccactaa
     4621 acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt ctgatgtctc
     4681 gatggctgtg ctgggcggca gcctgaaacg tcgtgagcgc atctcggcac gtctggggga
     4741 tattttaagc cagctttacc tcgcctctgc cgttctgaag cgttatgacg acgaaggccg
     4801 taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt atcaggctga
     4861 acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa
     4921 tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc tggatcataa
     4981 agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc gcggtcagta
     5041 cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg tggatgtgat
     5101 tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc tgccgtttac
     5161 ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata aagatgaagc
     5221 cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg atgactttga
     5281 tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga aagttgaagc
     5341 cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat tatttaaaat
     5401 taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc accgttttac
     5461 catcaatcga aagcgcatcg ttcacattca accaggtgag tttctcttgc gacgttttct
     5521 catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac atcacagcga
     5581 tacgttgcga gcaacagtca tgcggtttgc acgcgctcat cacctgatac gtctcatctc
     5641 ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag gcaggcagct
     5701 tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt gcaaggctcc
     5761 taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg gcgactgtcg
     5821 ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta accttctaaa
     5881 gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca tttgttatca
     5941 atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa cccttcgcat
     6001 aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac cactggtgtg
     6061 gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg
     6121 cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag cggcagcttc
     6181 gtcgctagca caagatgacg tagtgaactg gatgacagct aaggcgcagc agtgcaatgc
     6241 gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc gctttttgct
     6301 ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc gcatggcaga
     6361 tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc gcggctggcg
     6421 tattttgccg ctggtgtgct acgacttacg ttttcctgtg tggtcgcgca atctcaacga
     6481 ttatgacctc gccctgtacg tcgccaactg gcctgctccg cgctctctgc actggcaggc
     6541 attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg
     6601 cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc cacaaggcga
     6661 gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgccgagc tgtcgatggc
     6721 ggtgttaagg gaatatcgcg ataagtttcc ggcatggcgg gatgcggatg agtttaggtt
     6781 gtagtgagtg ggggctgtat ttagggcggg gaattcagaa agggatgttt agtgtgctga
     6841 gcgagagtta aataataaaa aactgggaag atttaataat ccccccgcag ggcaagagca
     6901 tgaattaaat ttgtgttaaa tttttcattt tatcagttcg aaacccaagc tctgagcagg
     6961 atgcaagcac ttcttctata taacatgtgt ttaacgccag ggcaagatta cgaaagcccg
     7021 caccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg catcttacgt
     7081 cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat gtgccgtatc
     7141 cctgaaaata attctgctgc atttcctctt cttattttgc agtcgtcttc attcattacc
     7201 acgtccagac gccagtgcag cttattctcc acgtgccagt ggtttcggat tgctgtggcg
     7261 aacttctctg cggttaaatc agcagaactg atataatatc tgaccgtcat ttctggctcc
     7321 ttcttttgtt ctgctattat ggaccgaaag gagactgcca cgcataattt cttcagccct
     7381 ttccattcaa acgtgaaatc aataagttca tcaaggacat cgcaaacaat atgaagacgg
     7441 atttcttctc tgccgtgact cttttcacta attgcgtaac tgtcatgctc tggattattt
     7501 aattctttca gcggaaattt ttcctcaaag gctttatt
//
LOCUS       DAZFAH010000202         7534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFAH010000202 DAZFAH010000000
VERSION     DAZFAH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.204"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..488
                     /gene="dicB"
                     /locus_tag="ACHJWB_004078"
     CDS             300..488
                     /gene="dicB"
                     /locus_tag="ACHJWB_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4315617.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            485..676
                     /locus_tag="ACHJWB_004079"
     CDS             485..676
                     /locus_tag="ACHJWB_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4315618.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKKVSLYLNNETPAG"
     gene            769..3213
                     /locus_tag="ACHJWB_004080"
     CDS             769..3213
                     /locus_tag="ACHJWB_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4315619.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNSEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHKTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDNASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQ
                     NDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGHSGGGNCPDCGAVMGDATYQ
                     EIFDGENQPEVQENDPEEMEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHKS
                     TSTSRVWIHLSVDLETMGTNPDAPINSIGGKFFDPATGEMGPEFSKAIDLETSGGIID
                     RKTIKWWAKQSREAQSAIFTDEISLDVALRLFIEFIEKNSGGCFVQVWGNGANFDNVI
                     LRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNALDDARHQAK
                     QVSAIWQKLFPNQADF"
     gene            3284..3535
                     /locus_tag="ACHJWB_004081"
     CDS             3284..3535
                     /locus_tag="ACHJWB_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4315620.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            3571..4851
                     /locus_tag="ACHJWB_004082"
     CDS             3571..4851
                     /locus_tag="ACHJWB_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO4315621.1"
                     /translation="MKYPTGVENHGGTLRIWFVYKGVRVRENLGVPDTVKNRRIAGEL
                     RASVCYAIKTGIFDYAKQFPYSHNLGKFGEARQNLTIGELAEKFLALKEAEVARSSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            5029..5271
                     /locus_tag="ACHJWB_004083"
     CDS             5029..5271
                     /locus_tag="ACHJWB_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4315622.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(5282..5353)
                     /locus_tag="ACHJWB_004084"
                     /pseudo
     CDS             complement(5282..5353)
                     /locus_tag="ACHJWB_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            5438..5521
                     /locus_tag="ACHJWB_004085"
                     /pseudo
     CDS             5438..5521
                     /locus_tag="ACHJWB_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(5642..6656)
                     /gene="nleC"
                     /locus_tag="ACHJWB_004086"
                     /pseudo
     CDS             complement(5642..6656)
                     /gene="nleC"
                     /locus_tag="ACHJWB_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            complement(7059..7328)
                     /locus_tag="ACHJWB_004087"
     CDS             complement(7059..7328)
                     /locus_tag="ACHJWB_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4315623.1"
                     /translation="MNVLKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(7330..>7534)
                     /locus_tag="ACHJWB_004088"
     CDS             complement(7330..>7534)
                     /locus_tag="ACHJWB_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4315624.1"
                     /translation="NIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT         2191 a         1760 c         1676 g         1907 t
ORIGIN      
        1 tgaggacacc gggttgtcag gttgaccata cgcttaagtg acaaccccgc tgcaacgccc
       61 tctgttatca attttctggt gacgtttggc ggtatcagtt ttactccgtg gctgctctgc
      121 cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac gcggaacagt
      181 taaaaccaaa aacagtgtta tgggtggatt ctctgtatcc ggcgttaatt gttaactggt
      241 taacgtcacc tggaggcacc aggcaccgca tcacaaaatt cattgttgag gacgcgataa
      301 tggaaacgtt attaccaaac gttaatacgt ctgaaggttg ttttgatatt ggtgttctgc
      361 tcagtaaccg ggagtttact gaagatgcca ttaatatgag gaaatatgag ccttatctgc
      421 tcaatgataa ttccatactt tcccgaattg ctcttcttga acttggtatt ttcggagaac
      481 gtcaatgact tcagcatttg cactgatgat gacggttttt cttataacgg gtgaatcaca
      541 gaatgtgatt accggaattt atgccagtaa agaatcctgc ctccaggcaa gagacgagca
      601 aaaaatttct ggtgaatgcc tcccggtaaa aaaaaaagta tcgctgtacc tgaataacga
      661 aacaccggct ggataaccct ccagccatat taacaccata ccaacggatt aaaaatgcca
      721 gcaatggcag ggatttgttc acccttaaat ctgtaatgag gtttatcaat gagcactgat
      781 aaagaagaat ttgcgctata ttgcgaagca aaaaatgaca aagtcagaaa acgtctggga
      841 attaaaggtg gtttttactg gactacagca aaaaaattat ctgttgccat ctcccgctgc
      901 attaccgcaa tggatgacaa cgattatgat gaagacgact ttaaaaaacc cgttcgcgtc
      961 catttacccg ttgtgaatga ccttccacct gaaggcgtgt ttgataccga attctgcaac
     1021 cgatacgaaa aaggcgggga agatggcatt acaatggtat ttatcgcgcc ctctccctcc
     1081 gtgcaggaga aaccagccag tactgacaat accaacgtca acagcgaaga catgacggag
     1141 attgaggaga atatgctcct gccggtttct ggtcaggagc tgcccattcg ctggcttgcg
     1201 caacacggca gcgaaaaacc agtaacgcac gttgcacggg aagaacttca ggcattacat
     1261 atagcacggg cggaagaact gcctgctgtt actgccctgg ccatttctca caaaacaaag
     1321 ctgctcgacc cgctggagat tcgcgacctt cacaaactgg tacgcgacac agacaaagtt
     1381 ttccctaatc ccgttaattc cagtctgggg ttaatgactg cttttttcga agcatacctg
     1441 gacgctgact ataccgatcg aggtctgctg acaaaagagt ggatgaaagg aaatcgtgtt
     1501 ttacgcatca gccgcacgcc atccggcgct aatgctggcg gaggaattct taccgatcgc
     1561 ggtgaaggtt ttgtccacga taatgcgtca gtggaacgtg acgttgccgc tggcgttctg
     1621 gcccgttcaa tggacatcga tatttacaat ccacatccgg cacacgccaa acgcattgaa
     1681 gaaatcgttt cagagaataa gccgcccttt tctgtttttc gtgacaaatt catcgccatg
     1741 cctggtcacc tggattattc ccgcgcgata gtggttgcgt ccgtgaaaga agcaccaatt
     1801 ggtatcgagg ctactcccca ccgtgttacc gaatatctga acaaagtact gaccgaaacc
     1861 gaccatgcca accctgatcc agaaatcgtg gatattgcct gcggtcgctc ctctgctcca
     1921 atgccgcagc gtgtaacaaa agaaggaaaa caggatgatg aagaaaaacc gcagccatct
     1981 ggcgcaatgg cagatgaaca ggcaacgact gaagcagtgg aaccggatac aactgaacat
     2041 aatcaggaca cgcagtcgat ggatgctcag ccacagataa attctgttga tgcgaaatat
     2101 cagaaactgc gggcagaact ccatgaagcc cggaaaaaca ttccgcccca aaatcctgtc
     2161 gatgcagaca aattactggc tgcctctcgc ggagaatttg ttgaagggat tagcgacccg
     2221 aatgatccga aatggattaa ggggatccag acccgcgatt ctgtgtacca gaatcagcca
     2281 gaaacggaac agaacgacca gaaagcggaa caaaacagcc caaatacgca acaaaacgag
     2341 ccagaaacga aacaacctga accagtagtg caacaggaac cggaaaagat ctgcaccgcc
     2401 tgcggtcata gcggtggcgg caactgccct gattgtggtg cggtgatggg tgacgcaaca
     2461 taccaggaaa tattcgatgg agagaatcag cctgaagttc aggaaaatga tccggaggaa
     2521 atggaaggcg cggaacatcc gaacaatgag aatgctggta acgatcagca tcacaccagc
     2581 gatagtgaaa ctggcgaggc ggcagatccc ttaattgcgg tgaacggtca tcacaaaagc
     2641 acatccacca gcagagtgtg gattcacctg tcggtcgacc ttgaaacgat gggaacaaat
     2701 cctgatgcgc caatcaactc tattggcggt aaattttttg atccggcaac cggagagatg
     2761 gggcctgaat tcagtaaagc tatcgatctg gaaacctccg gcggaatcat cgaccgtaaa
     2821 acaataaagt ggtgggcgaa acaatcacgc gaagcacaat ccgctatttt taccgatgaa
     2881 atctcgttag atgttgctct ccggctattc atagaattta tcgaaaaaaa ctccggcggg
     2941 tgttttgtcc aagtatgggg aaatggagcc aacttcgaca acgtaatttt acgccgttca
     3001 tatgaacggc aggggatccc ctgcccgtgg ctttactaca acgatcgcga tgtacgcaca
     3061 atcgttgaac tgggtaatgc cattggcttt gatgtcagaa tggctattcc attcgaaggc
     3121 gtaccccaca atgcgctgga cgatgcccgt caccaggcaa aacaagtttc agcaatctgg
     3181 caaaaactgt tcccgaatca ggctgatttt taatgttcaa ccgtcgccgg ttgtgactgg
     3241 tattctgcaa ccggcgctca tctgatgtaa gagataaaaa gcgatgagtg aagtaatcat
     3301 gattgtctct cccggcaaat gggtatctga agagcagtta attgcgctga aaggaataaa
     3361 aaaaggaaca ctgaaaaaag ccagggaaaa atcatttatg gaagggaggg agtataaaca
     3421 tgtcgctcat gacggcatgc cgtgggataa cagcccatgt ttttacaatc tggaagaaat
     3481 tgatcgctgg atcgagcgtc aggcatcggc aaggccaaga cgtcaccttg cttaatccaa
     3541 aacaacacta atcaacgaga gaaagctggc atgaaatatc cgacaggcgt agaaaaccac
     3601 ggggggacat tacgcatctg gtttgtttat aaaggcgtaa gagtcaggga aaacctcggt
     3661 gtccctgaca cagtaaaaaa cagacgcatc gctggtgaac tacgcgcttc tgtttgttac
     3721 gcaataaaaa cggggatttt tgactacgca aaacagttcc cctactccca caatctggga
     3781 aaatttgggg aagccagaca aaatttgact ataggtgaac tggccgaaaa atttctggct
     3841 ctaaaagagg ccgaagttgc cagaagttca ctcaatacat accgtgccgt catcaaaaat
     3901 atcctgagca taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg
     3961 gaggttcgta aagagttact gactggatac cagatcccca aaagtaacta tattgttaca
     4021 caaccaggga gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc
     4081 cagtttggtg ttgataacgg ttacctggca gataatccgt ttaaggggat ctcgccatta
     4141 aaggaatcaa gaaccattcc ggatcctctt tcgcgggaag aatttatccg tcttatcgat
     4201 gcgtgcagaa atcagcaagc aaaaaattta tggtgtgttt ctgtttatac tggagttcgc
     4261 cctggtgagc tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg
     4321 atcaggagaa atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat
     4381 cgggtcattc atcttattaa gccagcaatc gacgctctcc ggagtcagat gacattaacg
     4441 agactgagca aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa
     4501 caaaaatgca cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac
     4561 cattttaccg ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc
     4621 cgccatcgaa aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt
     4681 gctaacccgg catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag
     4741 gtatacggaa aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag
     4801 ttaagcgagt ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt
     4861 tatcaaatag ttaacacgca tgactcttga aatccataat tcatagatgt tttttactat
     4921 tctgtgggtt tttgggtgtt ttctaagttt tttcagatgg ttgtattttt tctaaaaatc
     4981 cctaatctcg attttgctgt ttatttgagg cctttttatg tcccatatat gccccacaga
     5041 taccccgcag ccaaaatcaa caaaatgcca aaaggttctg ttcctgccct gcaacaagaa
     5101 atgctgcgac gtgtcagtaa acgttatgac gatgtagaag tgatcatcaa atccaccagc
     5161 aacgatggcc tttcagttac tcgcaccgcc gataaagatt ctgcaaaaac ttttgttcag
     5221 gagacgctga aagatacctg ggagtctgct gacgagtggt ttgttcgcta ataaacacgt
     5281 aaatcggtaa cggctggaaa tcattcaata ctcgcactat cgaaagttca ccagccaacc
     5341 gcgacacgct cttacatacg aagtgccgcg ctttccttaa taatttttta gcagtactgt
     5401 gtaaatgatg agcgacctaa tccatgcatg acgcgtagtg cctattgtgc atctttctgc
     5461 gtctcttttt actggaccca agccaatgat gctggcgata ccattcccgc caggcccact
     5521 tacaacagta aaattcgggg ctctgccttt acatacaata caatgttgca cgattgccca
     5581 tcatcaactc cgttaatatc tatgagtagc aaccctatgt aactgtaaag acgaatcagc
     5641 gctaattgtg tctgtccaca tccccaaatg taaagatata aggagcacca tgatacagtt
     5701 ggatctgatt tacatcctgt aaattctcgt cgatagccac ccctggaaaa tcatgttgct
     5761 ggataaatcc gtatcctatg ttagacacag cggaatactc tgtgaaatca ggactcgcct
     5821 catatcggtc actgatcgta cccagtcttt tcgaagaaag ccctctcatt cttttcatgc
     5881 ctcatggcag cctgtcgaag ggcattcagg ttacgtagtc taagatgagc ttctcgttct
     5941 ggctctgcat atcctttgaa gtcgggaaca ctccatccca gctcttgagc gacacgacgt
     6001 gcgagaatct cggtgggtcc tagctctata ttactatctc cagatggatc gctggcccca
     6061 gtaacatgat gaataatctc gtgaatcagt ccttcctgcc acgatggcat ctcataagaa
     6121 tcagtatctg gcgcaacact aaaactgaca taaggctctt cgttctcgtt ttctcctgcc
     6181 tcgcaaatgg ggagaattgg ttcttgcccg acatcgtatc catataattc gttacaggtt
     6241 agtgattgaa tatcatcaat tttgatagca gattctccgt taagctcgta ttcgtttctg
     6301 tatttaatgc aaccaatgtg tacctcctca ttatgaatgc catagcttac ggcatcacga
     6361 aatgtttgtg atcggctcaa cgcatcaagt acagtgttgc caatcatatc gaccgtatgc
     6421 tgatcgatga gcctgctacg gctgtcatgg acggcgcgaa ttacactttc gtatacgttg
     6481 cttaaatctg ctgcggaaag cggtattcgc ttgcctatat ccaaaacgta atccgcatag
     6541 gcattttcag cacgattagg agcaacggca gcagagtatc cagcaggggc aaaaaagttg
     6601 aagccaggtt ggagaacggg aattttcata ttgatactta atatggtttt atatatttca
     6661 gtgccaccaa ttcactagct tacaaccaga aactaaaaaa acgtcagggc gttgataata
     6721 atgaatacca actttctgcc catttatagt aatatctttc gttatcaggt tttgtctggc
     6781 agcttcacgt aatatttcaa tactctgcaa agcaacattt gatgaaatgc tggatgttat
     6841 caatggcata tgtcatttac cccaacaaga ctaaatttta gaaaaaacaa ctgccgggac
     6901 aactttcgcc ccctgtaacc ccaaataaga tgtcagattt atcggcatta atttatctcc
     6961 ctgttgaatt agctcctcta tcatctactt aacaaatatc ccccggacat tgcaacacaa
     7021 aaaccggagc cggactccgg tttttgtgaa gctgtcggct atttcattcc gccaatattt
     7081 tcccacctcc cgtcagcacg caggatttgc agcggtctta ccacgcactg tatctgcttt
     7141 ttatccgcat ccagtatcac cacctgcgtg attaccctgt cctgctccgg gataatgcca
     7201 ttctcatcgg actccagaat gtctgccggt cccagtcgca gctgtgctgt aagtaactcc
     7261 ccgttttcac ggtcatcatg ctttccgcaa ccgcacagac gctgcataag tttttttagt
     7321 acgttcatgt cattctcctg ttctgcctgt atcactgccc acttcatcca gtcccttgac
     7381 atcctgccac ggcccgtcac caaacctgac ctgcaaatgc tgaaaaaaac cctgaacccg
     7441 tgtggcatct ttggggtcaa gaaaggtcag tccggtgatg agtgcgccat ctgtatccgg
     7501 gaaccagcca tggctgtttg tctcaataat gttt
//
LOCUS       DAZFAH010000203         7521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFAH010000203 DAZFAH010000000
VERSION     DAZFAH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.205"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            176..4429
                     /gene="fdeC"
                     /locus_tag="ACHJWB_004089"
     CDS             176..4429
                     /gene="fdeC"
                     /locus_tag="ACHJWB_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO4315625.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGDWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAPAQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITALAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4550..5407)
                     /locus_tag="ACHJWB_004090"
     CDS             complement(4550..5407)
                     /locus_tag="ACHJWB_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4315626.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5656..6525
                     /locus_tag="ACHJWB_004091"
     CDS             5656..6525
                     /locus_tag="ACHJWB_004091"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO4315627.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGELVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6685..7278)
                     /gene="rclC"
                     /locus_tag="ACHJWB_004092"
     CDS             complement(6685..7278)
                     /gene="rclC"
                     /locus_tag="ACHJWB_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO4315628.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWKTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7290..>7521)
                     /gene="rclB"
                     /locus_tag="ACHJWB_004093"
     CDS             complement(7290..>7521)
                     /gene="rclB"
                     /locus_tag="ACHJWB_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO4315629.1"
                     /translation="KKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDT
                     IADIARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2042 a         1669 c         1932 g         1878 t
ORIGIN      
        1 cctgatgagt ggctacaggc actggatata ttaggtggcg gtgtactttc ttacataaag
       61 gtatttcctt ttctgcggaa aaggaaaccg ggaaatctcc ggtttttctg acaagcagac
      121 gccattattt gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc
      181 acgttataaa acaggtcata aacaaccact atttcgttat tcagttctgg cccgctgcgt
      241 ggcgtgggca aatatctctg ttcaggttct ttttccactc gctgtcacct ttaccccagt
      301 aatggcggca catgcgcagc atgcggttca gccacggttg agcatggaaa ataccacggt
      361 aactgctgat aataatgtgg agaaaaatgt cgcgtcgttt gccgcaaatg ccgggacatt
      421 tttaagcagt cagccagata gcgatgcgac acgtaatttt attaccggaa tggccaccgc
      481 taaagctaac caggaaatac aggagtggct cgggaaatat ggtacagcgc gcgtcaaact
      541 gaatgtcgat aaagatttct cgctgaagga ttcttcgctg gaaatgcttt atccgattta
      601 tgatacgccg acaaatatgt tgttcactca gggggcaata catcgtacag acgatcgtac
      661 tcagtcaaat attggttttg gctggcgtca tttttcagga aatgactgga tggcgggggt
      721 gaatactttt atcgatcatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga
      781 atactggcgc gattatctga aactgagcgc caatggttat attcgggctt ctggctggaa
      841 aaaatcgccg gatattgagg attatcagga acgcccggcg aatggttggg atatccgcgc
      901 agagggctat ttacctgcct ggccgcagct tggcgcaagc ctgatgtatg aacagtatta
      961 tggcgatgaa gtcgggctgt ttggtaaaga taagcgccag aaagacccgc atgctatttc
     1021 tgccgaggtg acctatacgc cagtgcctct tctgacactg agcgccgggc ataagcaggg
     1081 caagagcggt gagaatgaca ctcgctttgg cctggaagtt aactaccgga ttggcgaacc
     1141 tctggagaaa caactcgata cagacagcat tcgcgagcgt cgcatgctgg caggcagccg
     1201 ctatgacctg gttgagcgta ataacaacat cgttcttgag taccgcaaat ctgaagtgat
     1261 ccgtattgct ctgcctgagc gtattgaagg caagggcggt cagacacttt ccctcgggct
     1321 tgtggtcagc aaagcaactc acggactgaa aaatgtgcag tgggaagcgc cgtcattact
     1381 ggctgaaggt ggcaaaatta ccggtcaggg tagtcagtgg caagtaacgc tcccggctta
     1441 tcgtccaggc aaagacaatt attatgcgat ttctgcggtt gcctacgata acaaaggcaa
     1501 tacctcaaaa cgcgtgcaga cagaggtggt cattaccgga gctggtatga gcgccgatcg
     1561 cacggcgtta acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca
     1621 aaaaccgctg gtgctgtctc tgcgcgacgc cgagggccag ccagtcacgg gcatgaaaga
     1681 tcagatcaag actgaactaa ctttcaaacc ggctggaaat attgtgactc gtaccctgaa
     1741 ggccactaaa tcacaggcaa agccaacact gggtgagttc accgaaactg aagccggggt
     1801 gtatcagtct gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt
     1861 tgatggcatg agcaaaaccg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa
     1921 ctccaccctg agcgctaacg agccgtcagg tgacgtggtt gctgatggtc agcaagccta
     1981 tacgttgacg ttgactgcgg tggactccga gggtaatccg gtgacgggag aagccagccg
     2041 cttgcgattt gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa
     2101 accaggcgtt tacagcgcca cggtttcttc gacccgtgcc ggaaacgttg ttgtgcgtgc
     2161 tttcagcgag cagtatcagc tgggcacatt acaacaaacg ctgaagtttg ttgccgggcc
     2221 gcttgatgca gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttggcggtac
     2281 agttacggca atctggacgg caaaagatgc taatgacaac cctgtaactg gtctcaatcc
     2341 ggatgcaccg tcattatcgg gcgcagctgc tgtaggttct acggcatcag gctggacgga
     2401 taatggcgac gggacctgga ctgcgcagat ttctctcggc actacggcgg gtgaattaga
     2461 ggttatgccg aagctaaatg gacaggatgc ggcagcgaat gcggctaaag taaccgtggt
     2521 ggctgatgcg ttatcttcag gccagtcgac agtgtctgta gccgcagatc gcgtaaaagc
     2581 aggcgaaagc acaaccgtga cgcttattgc aaaagatgca catggcaacg ctatcagtgg
     2641 tctttccctg tcggcaagcc tgacgggtgc tgcgtctgaa ggggcgactg tttctggttg
     2701 gaccgaaaaa ggtgatggtt cctatgtcgc tacgctgaca acaggtggaa agacgggtga
     2761 gcttctcgtc atgccgctat tcaacggcca gccagcagcc accgaagccg cgcagttgac
     2821 ggtcatcgcc ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaggctcc
     2881 gaccgtcaaa acgacgacgg aactcacctt cactgtgaag gatgcgtacg ggaacccggt
     2941 caccgggctg aagccagatg caccagtgtt tagcggtgcc gccagcacgg ggagtgagcg
     3001 tccttcagca ggagactgga cagagaaagg taatggggtc tacgtgtcga ccttaacgct
     3061 gggatctgcc gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc
     3121 tcagccactg gtgctgaatg ttgcaggtga cgcatctaag gctgagattc gtgatatgac
     3181 agtgaaggtt aataaccaac tggctaatgg acagtctgct aaccagataa ccctgaccgt
     3241 tgtggacagc tatggtaatc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg
     3301 tgtgaccagc aagacgggga atacagtaac aaccaatgcg gcagggaaag tggacattga
     3361 gcttatgtca acggttgcgg gagaacacaa tatttccgct tcggtgaatg gtgctcagaa
     3421 gacggtcacg gtgaaattca acgcggatgc cagcaccggt caggcaaacc tgcaggtaga
     3481 cgcccctgct caaaaagtgg caaacggcaa agatgccttt acgctgacgg cgaccgttga
     3541 ggataaaaat ggtaaccctg ttccagggag cctggtgacc tttaatctgc cccggggtgt
     3601 caagccgctt acaggcgata atgtctgggt gaaagccaac gatgagggga aagccgagtt
     3661 gcaggtggtt tcagtgactg ccggaacgta tgagatcacg gcattggcag ggaatagcca
     3721 gccttcgaat acgcagacta taacgtttgt agccgataag gctaccgcaa ccgtctccgg
     3781 tattgaggtg atgggcaact atgcgctggc ggacggcaaa gccaaacaaa cgtataaagt
     3841 tacggtgact gatgccaata acaatttggt gaaagatagc gaagtgacgc tgactgccag
     3901 cccggcttcg ttaaacctgg aaccgaatgg cactgcgaca acgaatgagc aagggcaggc
     3961 tattttcacc gctaccacta ctgttgcggc gacatacaca ctcaaggcgc aagtgagtca
     4021 gaccaacggt caggtatcaa cgaaaactgc cgaatctaaa ttcgttgcgg atgataaaaa
     4081 cgcggtactc accgcatcat ctgatatgca atctctggtg gcggatggga aatcgactgc
     4141 gaagctggag gtgacactga tgtcggcaaa caaccccgtt ggcgggaata tgtgggtcga
     4201 cattcagacg ccggaagggg tgacggagaa ggattatcag ttcctgccgt cgaaaaatga
     4261 ccatttcgtg agcggaaaaa tcacgcgtaa atttagtacc agcaagcctg gtgtctatac
     4321 gttcacattt aacgccctga cctatggcgg gtacgaaatg aagccagtga cggtgaccat
     4381 taccgcggtg gatgccgata cggcaaagga cgaggaggcg atgaaataat tttaagaata
     4441 tgaaagtaac attctaataa acaataatgg cgttgacatc ttcaacgcca ttatttattg
     4501 aatttcctga cagtagtctc gttataaatt catttagata tagcgaaaac taaaccggac
     4561 gaacggggat aaaaatttcg caaacaacat cattgctatc cttttctacg aaatcttcat
     4621 tgtaataaaa ccgctcaata tcactgccgt tcactctggc taaccccctg gcgggtaatt
     4681 ccatcagata gaggtttcgc gcccattcag ggtagtgctc ccactcacct tcatagcgga
     4741 aagaggcata cagtcctcgg ttaattgtcc actctttcgg catctctgaa tcggtttgtt
     4801 tgcataccgt accggcaaaa gtttcaaccg tgagcaaatc gaccgatttt gttgacggtt
     4861 caaacgtgga gacgaaatag atatccgtct taaaattatt catacatctg gctaaatgat
     4921 gacgtcgcag tttgacttct tcatccggtg atttattcga cacttcaatg tcatatttaa
     4981 atgagttgcc aaaaataggt gtctcaggaa aattgactaa tctacactcc gtcttttgct
     5041 gttgacgtat taaaaaagaa gggaagatat ttgcgagatc ccaataatca cggtggcggt
     5101 attcacgggg agagcagccg aataactttt taaattcgcg gctgaatgac tgctgtgaat
     5161 caaaatgcaa agagagtgca atatcaagca tagatttcgc ggtgagccgg acaagaatag
     5221 cggcacggca aagccttcgt ttgcgaatgt attctcccaa aggcacatgc atgaaatttc
     5281 ggaacagaag ctggatgttg cgtctgctgt agccagattt ctgtgcgata tcttcgattg
     5341 aaatagggtg ctcaagattg cactcaatcc actccaggag ctgctgtaga atcttctgcc
     5401 tgatcatcac attctccgtt aattctttgt catatcgcac attttcgtta tgccctgggc
     5461 cctaagtgta tcaaaaacag caagggcgaa ggcaaaagca ctggtagcgt ctgcttcgtt
     5521 gtccccgaaa atagagaggt ttcagtcctg cattattaat gaattttttg cataagtgat
     5581 atcaaaatcc acgtactaat ttgaggttac gttttaacgt agactcattg ttcatgccta
     5641 atggagggac tgacagtgga attttccgta ttaagtaaca atctgaaaat gccgatgatg
     5701 ggatttggtg tttttcaggt taccgataaa aacgtgtgca aacagtcagt gttgaacgct
     5761 atccgcacag gttatcgact catcgacact gcagcggtat acggtaatga agatgccgtc
     5821 ggtgaagccg ttcgtgaagc tatatctgaa ggtttatgta cccgagatga gttatttata
     5881 acgtctaaat tgtgggtgca ggatatgttg aatcaggata cggcagcagc aggtattgaa
     5941 gcatcattaa aaaaatcggg actagagtac ttcgacctct atttattgca ccaggctatg
     6001 cgcgattatt tcagtgcgtg gcgtgcactc gaagatgcct atgaagaggg caaattaaaa
     6061 gcaattgggg tttccaattt ctatcctcat gttctggcga acttttgtga aacggtaaga
     6121 gttaaaccga tggtcaacca ggtcgagttg catccctatt ttgcccaacc agaggcgctg
     6181 gcaaccatga agtattataa cgtgcagcct gaagcatggg ctccgttagg tggtggacga
     6241 cataaaccct ttgaaaataa tctgcttcag agtattgcag atgcccatca aaaatcgatt
     6301 tctcaagtca ttctgcgttg gaatattcaa cggggagtgg tcgttattcc gaaatcgaca
     6361 catcaacagc gtatcgaaga aaattttgct atctgggatt tctcactgac agagaaagaa
     6421 atggcacaaa ttagttcgct tgatttgggt tatgttgggg agttggtaaa acattttaat
     6481 cctgaatttg ttcgtggttg tcttgctgta aaaatacatg attgatatta atcatatatt
     6541 ttacctgaga cgacaagaat cttttaacag gggagtgata ttgatcttca ctctgtcata
     6601 tctccggtaa tatggcgtca ggcttcatca tcagaaaggg ggcctgacgc ctgaaaaagt
     6661 gaacaacaga cagtgttcgg attatcaata ttcagttttt aacgttgaac tggattcatt
     6721 actgcgttgt ttaagaattt cccgcgccga atctgccatt atcatgacag cacctgccag
     6781 catcagagta tctttcaata ccaggcgacc agcaccggat aaataaggga aaccatgatg
     6841 agcgtcaccc aatgcgggta cccatgcctc cggggtggtg attaaaaatg agagtgttac
     6901 cagcggcgtg gtaaatgcca tcagtcctcc caataaacct aaccagcgat tgacaggatt
     6961 agccaaaacc agcagagcaa taatcacctc cacgacgcca agaccgttgg aaaaaccata
     7021 ggtattattg gccgttttcc atgcccttgc ttctggtttg tattcgcctt cgtgagtcag
     7081 atactgttta tagtcttccg ggtgttcata aaagaacgac attagtggac tgtttgcgac
     7141 gaatggtgta atgctgtctg cctcgtaagg gacaaacttt aataacccaa tccacataaa
     7201 aacaattgca atactcagac gaatcaatgt caggccaatt ttatctcccc gacttaacag
     7261 gtgcaggtat ttttccattg tgaacatcct tatttataca atactgctgt tgcggttgag
     7321 ttattatcaa ttcgcatcga tgtgactttc caggatgaag cattatattt tctggctata
     7381 tcagcaatgg tatcctccag aactgtcggt gatgctgata ctgacgagac gctgatatgt
     7441 ttcagaatta ttttatcatc tgcattggtg gaaaatgcca taacgccaga taatagtgtt
     7501 gcaaataaaa cagatttttt a
//
LOCUS       DAZFAH010000204         7433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFAH010000204 DAZFAH010000000
VERSION     DAZFAH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.206"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..406)
                     /gene="yajD"
                     /locus_tag="ACHJWB_004094"
     CDS             complement(59..406)
                     /gene="yajD"
                     /locus_tag="ACHJWB_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4315630.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(535..1506)
                     /gene="secF"
                     /locus_tag="ACHJWB_004095"
     CDS             complement(535..1506)
                     /gene="secF"
                     /locus_tag="ACHJWB_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4315631.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1517..3364)
                     /gene="secD"
                     /locus_tag="ACHJWB_004096"
     CDS             complement(1517..3364)
                     /gene="secD"
                     /locus_tag="ACHJWB_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4315632.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALSKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(3392..3724)
                     /gene="yajC"
                     /locus_tag="ACHJWB_004097"
     CDS             complement(3392..3724)
                     /gene="yajC"
                     /locus_tag="ACHJWB_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4315633.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3747..4874)
                     /gene="tgt"
                     /locus_tag="ACHJWB_004098"
     CDS             complement(3747..4874)
                     /gene="tgt"
                     /locus_tag="ACHJWB_004098"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4315634.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPSLNVD"
     gene            complement(4930..6000)
                     /gene="queA"
                     /locus_tag="ACHJWB_004099"
     CDS             complement(4930..6000)
                     /gene="queA"
                     /locus_tag="ACHJWB_004099"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4315635.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            6093..6674
                     /gene="acpH"
                     /locus_tag="ACHJWB_004100"
     CDS             6093..6674
                     /gene="acpH"
                     /locus_tag="ACHJWB_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4315636.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6679..>7433)
                     /locus_tag="ACHJWB_004101"
     CDS             complement(6679..>7433)
                     /locus_tag="ACHJWB_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO4315637.1"
                     /translation="AGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTF
                     PLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLL
                     GRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALY
                     QRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLPASPF
                     LNVAQWQRKEGHGQLTNGILALPAISATVWMN"
BASE COUNT         1805 a         2034 c         1818 g         1776 t
ORIGIN      
        1 ctggagggat aatacaaggc ctgataagcg tagcgcatca ggcaatttta cgttttaatc
       61 acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga
      121 catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat
      181 atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta ctgccatctt
      241 ccgggttatt ggtatggtcg tgatcaatgt ggtgaacagt aagttcacgc aggttggaat
      301 aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc
      361 gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaag agttacctgg
      421 atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca
      481 tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattacgg
      541 cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg
      601 cttcataccc agtttcagag ccaacgcaga tgccacatag atggaagatg cagtaccgat
      661 ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagaggta
      721 cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg
      781 ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc
      841 acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa
      901 cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat
      961 gataacgtcg tgcgccagcg caataacaac ccctgccgcc agtcgccact caaagcgaaa
     1021 acctacgtac acaaggatag acagcagcgc cgccatcaac gccatcgcac cggtttgcgc
     1081 aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt
     1141 ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccgc cggtttcgcc
     1201 ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg
     1261 ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc
     1321 gagcgtaatt tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac
     1381 gcccataata acgatagcgg cgattaacag cagaccagag atgccgaaag cccagtagtc
     1441 ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg
     1501 tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt
     1561 ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg
     1621 tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa
     1681 tcagcgtggt gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa
     1741 ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta
     1801 cgttcgcatc gaccgccacc gcaagggtta agacgatacc cgcgatacct ggcatactca
     1861 gcgttgcgcc tggcaacagc gacataatgc cgacgattaa gatcaagttg gcaatcagag
     1921 cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca
     1981 gcagaccggc aaggcaagcc tccagcccct gttcaatgtt ctgcataccc agggttgggc
     2041 caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca
     2101 gtgacagctg acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca
     2161 gacgagactg gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac
     2221 catttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc
     2281 cgatgttgtc cttagtgaag ttagacatga tgttaccacc agcgctatcg agcgagatgt
     2341 taacctgcgg ttggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg
     2401 tcagaattac gcgtttgtac agcacaactg gctgaccttc gcgggtctgt ttcacttcag
     2461 agtcgcccgg tacgcgaccg gatgccgcag cggcctggtc aacgttggtg tttaccagac
     2521 ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg tcctgaatac
     2581 ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc
     2641 caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca tattcacgcg
     2701 cttcactcag acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctactaa
     2761 tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat
     2821 cgcggaaagt aatgctcagg ccgtagttgt tttctttacg aacggtggta tacgggatgc
     2881 ccttttcacg cagatcgctg cgcaggctat cgatattttg ttcctggagt ttgctaagcg
     2941 cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct
     3001 tcatcggctc agcgtgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg
     3061 ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc agctgggtgt
     3121 cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta
     3181 ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc
     3241 cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca
     3301 gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta
     3361 acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca
     3421 ggacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag
     3481 caatgtagcc gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa
     3541 cttcatcacc tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct
     3601 gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag
     3661 acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc
     3721 tcattaaatt tccctcatta ttaatattaa tcaacgttca aagatggtac ttctcgcccc
     3781 tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt
     3841 aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct
     3901 aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg
     3961 tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc attgcggatt
     4021 ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg
     4081 cagtcaaaca tatcgatacc acgacgtacg ccttcaacca ggtcttctgg tttaccaacg
     4141 cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc cagaatgcgg
     4201 tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaaaccg
     4261 atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacgct gccctgaatg
     4321 ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc
     4381 caacgcagag acatctccat ggagcgtttt gcgtaatccc agtcagcagg atacggcgta
     4441 cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc cattgatttt
     4501 tcaggatcga ggaaaatcgg atcgccgttg atcgggttac ggaagtgcac gccctgttcg
     4561 gtgattttac gaatatcgcc aaggctgaag acctggaagc cgccggagtc ggtgaggatc
     4621 ggccccttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc
     4681 gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact
     4741 tcttccggcg tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt
     4801 tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc
     4861 agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc
     4921 gccgcggaat tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat
     4981 cgcatcaccg taactaaaaa agcgatattt ctcttctacc gctgctttat aggcgttcat
     5041 ggtgtgttga taaccggcaa aggccgaaac cagcataatc agcgtcgact ctggcaagtg
     5101 gaagttcgtc accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg
     5161 ggtatcgtcg aagaacggtt caatgagatc gtttttcgct gcctgagccg cgctttccag
     5221 cgaacgtact gaagtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag
     5281 taccgcgtct accacatcct gcggtacttc agcgtattcc gagtgcatga tgtgatcttc
     5341 aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc
     5401 catctccacg cctttggcgc gcaatttttc cagcaaaggc tcgtcaaaat gcagaccagc
     5461 ggtcggggct gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc
     5521 agcgtcttcg tccggacggt cgatatacgg cggcagcggc atatggccga tactgttgag
     5581 gatatccagt accgaacgat catcattgaa ttcgacttca aacagtgcgc cgtggcgcgc
     5641 ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg
     5701 cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag
     5761 cacttcaatc ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt
     5821 attattaaaa accagaagat cgccggggtt gagcttatca agtaaatcgg tgaaagtacc
     5881 gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg
     5941 catgggatag tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat
     6001 gacactgact cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca
     6061 acctttaccc ccttttggat agaatataat cgatgaattt tttagctcac ctgcatttag
     6121 cccatctcgc ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc
     6181 ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg
     6241 tattaaccga caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaactc
     6301 gccgcgttgc gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt
     6361 cgcagctgtc gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga
     6421 tgacgatttt gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag
     6481 agcagtggct ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg
     6541 caagccgccg cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt
     6601 atgacgccct cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac
     6661 acaaggcgtt ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag
     6721 aatcccgttt gtcagttgcc catggccttc tttgcgttgc cattgcgcga cattaagaaa
     6781 cggtgacgcg gggagcacca cttcacaggc ctcgccacgg ttgattgcca ccagtacacg
     6841 ttgctgattc agcacgcgga caaataccac tacgttatct tccgcataca gcacctgaca
     6901 gccgccacga cgcagcgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc
     6961 gaataacgcc gtatcctgct tttccacctg ccaggggaac ggtttacggc aaaacggatc
     7021 gtttttgcca tccagtccta cttcatcacc gtaataaatg cacggtacac caggccaggt
     7081 gaacagccag accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa
     7141 tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag
     7201 ccctgcgcgg taattatcca tccaggccat acaggtttgg gcatcaattt gctgcggatc
     7261 gtaagagata tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt
     7321 catggcggca tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc
     7381 gacaatatac gcttccggct gggtttcttt cgccgcttcg gtgatcccag caa
//
LOCUS       DAZFAH010000205         7265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFAH010000205 DAZFAH010000000
VERSION     DAZFAH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.207"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..444)
                     /gene="folK"
                     /locus_tag="ACHJWB_004102"
     CDS             complement(<1..444)
                     /gene="folK"
                     /locus_tag="ACHJWB_004102"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4315638.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVL"
     gene            complement(441..1838)
                     /gene="pcnB"
                     /locus_tag="ACHJWB_004103"
     CDS             complement(441..1838)
                     /gene="pcnB"
                     /locus_tag="ACHJWB_004103"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4315639.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1898..2794)
                     /gene="gluQRS"
                     /locus_tag="ACHJWB_004104"
     CDS             complement(1898..2794)
                     /gene="gluQRS"
                     /locus_tag="ACHJWB_004104"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4315640.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2861..3316)
                     /gene="dksA"
                     /locus_tag="ACHJWB_004105"
     CDS             complement(2861..3316)
                     /gene="dksA"
                     /locus_tag="ACHJWB_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4315641.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3494..4198)
                     /gene="sfsA"
                     /locus_tag="ACHJWB_004106"
     CDS             complement(3494..4198)
                     /gene="sfsA"
                     /locus_tag="ACHJWB_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4315642.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSISDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4213..4743)
                     /gene="thpR"
                     /locus_tag="ACHJWB_004107"
     CDS             complement(4213..4743)
                     /gene="thpR"
                     /locus_tag="ACHJWB_004107"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4315643.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4817..7246
                     /gene="hrpB"
                     /locus_tag="ACHJWB_004108"
     CDS             4817..7246
                     /gene="hrpB"
                     /locus_tag="ACHJWB_004108"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4315644.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDLELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1647 a         1907 c         2035 g         1676 t
ORIGIN      
        1 taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa tctcaaacag
       61 cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca ggcgttcggt
      121 atttattact tcattaccaa acagcatgat gtcgagatcc agcgtgcgcg gtccccagcg
      181 ttccgcttta cggacgcgac cttgttgtaa ctcaatacgc tgagtgtgat tgagtagctc
      241 ttcaggggca agagaggttt ccagcgctac ggctgcattt aagtaatcgg gttgatcttg
      301 cggccccagc ggtggagtgc ggtaaaacga agaaacggca agaatgtggc tttcagggat
      361 atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc
      421 tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga
      481 cgacgagtac ggcgacgcgg tgacggctct tcatccaact cgttaagcat ccctttttga
      541 tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg cagttcagcg
      601 ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa cttaggatgc
      661 tccagcagtt tccatgcgcg tttaccctga cgtcgggaca tacgcaactg caactgccag
      721 atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca ggcttcgtcc
      781 agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg
      841 atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag gaacgccggg
      901 ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc
      961 cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc
     1021 tggaacagat gatattcaca caacagctta taggtttcgt aaccgtagcc cgcttgtagc
     1081 agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcagcag ggtagcgagg
     1141 cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc ggcaaaacgc
     1201 accgcgcgca gcatacgtac cggatcttca cggtagcgtg tttccgggtt accaatcaga
     1261 cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac ggtaaaatcc
     1321 gctacgctgt aatacaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg
     1381 gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg
     1441 tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca
     1501 aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg
     1561 cgcacctgct caggcgtggc gttagtggtt acgtcaaagt ctttcggctt tttgccaagt
     1621 aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt attgagcctg
     1681 tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc
     1741 gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt
     1801 agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa
     1861 catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg
     1921 ttgaatttac aattgccgac tcaggcacgg cggtcagcgt ccagtttttg acggctgact
     1981 gaaggatttg ttcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt
     2041 gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc gcatgattct
     2101 gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg taatctggca
     2161 ctttccagcc aaaaagctgg tacagcgaga tttgccgcac tgtcggttcg atcaaatctg
     2221 ccccgcggac aatttcgctc acgccctgga agtgatcatc caccaccacc gccaggttat
     2281 aggcaaacaa accatcacgg cgatgaataa taaaatcttc ccgtgccagt ttttcgtcgg
     2341 cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc tggcggatac
     2401 gtactgcggc gttgtctggt ccatgatgca actcccggca atgaccgtcg taaataccgc
     2461 caatgctttg aatacgcgca cgcgtgcagg tgcagtaata acttagtcct tgttcatgta
     2521 accaggcgag tgcttcacga taggcatcgt gacgttgcga ttgccagaga acatcgccgt
     2581 cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt
     2641 cacgaggcgg gtcgatatct tcaatacgta ccagccagcg accgtgccgg gcgcgagcct
     2701 gcaaatagct gccaagcgca gcaatcagag aaccaaaatg aagctcgccg gaaggagagg
     2761 gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga
     2821 gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt
     2881 cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggcagtcggg cgcgcttcca
     2941 gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca
     3001 cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca
     3061 gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg
     3121 cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg
     3181 cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct
     3241 tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt
     3301 tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg
     3361 aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac
     3421 acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca
     3481 gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg ccttcagcag
     3541 aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt
     3601 gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca
     3661 cggcgaaaaa gataaccgca cgttggcctt cagccgctac gctcatcaac tcccgaaggt
     3721 gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg
     3781 taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa
     3841 tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt
     3901 ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtgttga
     3961 cgcaaataaa tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt
     4021 tggtgttgtc tgaaatcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcatcg
     4081 cacccgtatt tgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta
     4141 aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa aattccattg
     4201 cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc
     4261 acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcatacgacc agttaaaacc
     4321 tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa
     4381 cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca tattcgccag
     4441 ctggattaag ccgcgtggcg gctgacgcat ccctaaccac accacacgcg aacgcagcca
     4501 ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag
     4561 agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag tcagatgcaa
     4621 attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat
     4681 aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt gcggttcaga
     4741 catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa
     4801 ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg aattacttac
     4861 cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg ggaaatcaac
     4921 ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctgctgga
     4981 gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg gcggagctgc ttaacgaaaa
     5041 gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg
     5101 cctggaagtg gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc tggaactgag
     5161 cggtgttgga ctggtgatcc ttgatgaatt tcatgagcgc agcttgcagg cggatttggc
     5221 gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat
     5281 gtcggctacc ctggacaacg accgcttgca gcaaatgctg ccagaagcgc ctgtcgtcat
     5341 ctcagaaggg cgctcgtttc cggttgaacg ccgttattta ccgctgcccg cgcatcagcg
     5401 ttttgacgat gccgttgcgg tagccaccgc tgaaatgctg cgtcaggaaa gcggatcatt
     5461 actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tggcttcgcg
     5521 catcggcagt gatgtattgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg
     5581 aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc
     5641 tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc aggagcgtgt
     5701 ggcgcgtttt gatccgcgca cggggcttac gcgactgatt actcaacgcg ttagccaggc
     5761 atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatca gcctgcattt
     5821 aatcgccaaa gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga tcttacaaag
     5881 cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat
     5941 gagctggctg gatcaaccgc cagtagtgaa tctactggcc gcgaaacgtc tgttacaaat
     6001 gctgggggca ctggagggtg aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg
     6061 taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg aagctgctac
     6121 cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg
     6181 cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg
     6241 cttaaacgta cgtggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggggc
     6301 gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac tggcaaacgg
     6361 catgggagcg atgctcgatg ccaacgacgc gctaagccgc cacgaatggt tgatcgcacc
     6421 gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc tgccggtcga
     6481 tattgatgag ttagtacaac gctgcccgca gctggtacag cagtctgaca ctgtggagtg
     6541 ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc tgctgacggt
     6601 gaaagtgcag ccgctggcga aaccctcgga agacgagttg catcaggcga tgcttaatgg
     6661 catccgcgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt
     6721 gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga
     6781 aagtttattg gcgacgctgg aaacgtggct gctgccacat atgactggcg tacattcact
     6841 acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg attggggaat
     6901 gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat
     6961 cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aagagatgtt
     7021 tggcgaggcc accaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggagttgct
     7081 ttcacctgcc caaaggccat tacaaatcac acgagatttg agcgccttct ggaaaggagc
     7141 gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga
     7201 cccggcaaat actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc
     7261 ctctc
//
LOCUS       DAZFAH010000206         7258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFAH010000206 DAZFAH010000000
VERSION     DAZFAH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.208"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(319..1455)
                     /locus_tag="ACHJWB_004109"
     CDS             complement(319..1455)
                     /locus_tag="ACHJWB_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4315645.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="ACHJWB_004110"
     CDS             complement(1458..1820)
                     /locus_tag="ACHJWB_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4315646.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHJWB_004111"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHJWB_004111"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4315647.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4500..5648
                     /gene="mtlD"
                     /locus_tag="ACHJWB_004112"
     CDS             4500..5648
                     /gene="mtlD"
                     /locus_tag="ACHJWB_004112"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4315648.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKTWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5648..6235
                     /gene="mtlR"
                     /locus_tag="ACHJWB_004113"
     CDS             5648..6235
                     /gene="mtlR"
                     /locus_tag="ACHJWB_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4315649.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKIRLKKAFQK"
     gene            complement(6247..6456)
                     /gene="yibT"
                     /locus_tag="ACHJWB_004114"
     CDS             complement(6247..6456)
                     /gene="yibT"
                     /locus_tag="ACHJWB_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4315650.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6741..7103
                     /locus_tag="ACHJWB_004115"
     CDS             6741..7103
                     /locus_tag="ACHJWB_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4315651.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1842 a         1826 c         1837 g         1753 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta ggatccagtt caatggttcc
      481 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      721 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca agtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta ctcacattta
     2101 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgcgatg
     2161 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcactac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgccattct ggcatttctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat ccatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagt gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat
     3121 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctgg ccgttatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccagc
     3241 atctccgggg tctatccttg cggtactggc gatgacgcca aaaggtgctt acttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggcg catctccgct gtctgctggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc
     3541 aggcgttctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttctg ttactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctga ccgaacgtct ggttgctgcc caacgccaca cggcaaacga
     3781 agagaaagta aaagacagcc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 gctaggcgcg gagaacatct tcctcggtcg caaagcggca accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtc gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgctgc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4261 tcgtaagtaa tccaatccca ccctctccac atggagaggg tggggctaat tgcctgatgc
     4321 gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgtttacgc cgcatccggc gctgcccctc tccacatgga gagggtttgg
     4441 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4501 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4561 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4621 atgcccgtca tagctatcag gttcatgtgg ttggtgaaac cgagcaggtt gataccgttt
     4681 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
     4741 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
     4801 aagggctggt gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
     4861 aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccagaag
     4921 acgccaaaac gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg
     4981 tgccgccttc ggcttcggca actaacgatc ctctggaagt gacggtagaa accttcagcg
     5041 aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca ggcatggagt
     5101 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5161 ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac gcgattctcg
     5221 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgca gtattgatca
     5281 agcgctacgg ctttgacgct gacaagcatg cggcgtacat ccagaaaatt ctcggccgtt
     5341 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagcca ctgcgtaaac
     5401 tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatat ggtctgccac
     5461 ataaaaacct gattgaaggt attgccgctg caatgcactt ccgcagtgaa gatgatccgc
     5521 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
     5581 tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa
     5641 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5701 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5761 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag ctcctgaccg aggcggtaaa
     5821 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
     5881 gttactcgac ggcgatggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5941 gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg cattgcgtga
     6001 agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc ttggaccctt
     6061 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6121 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6181 ttcactgact gagctgattt ccaaaatcag attaaaaaaa gcctttcaaa agtaagcaac
     6241 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
     6301 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6361 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6421 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6481 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6541 aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc
     6601 ttaattaatc aacgaattgt gtagtttttg agcatgttaa tgattacgtt acagaactat
     6661 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6721 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6781 atcgtctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca
     6841 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     6901 aagttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6961 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     7021 tggtggttgt gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg
     7081 gattctctaa gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca
     7141 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     7201 caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg cattttta
//
LOCUS       DAZFAH010000207         7220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFAH010000207 DAZFAH010000000
VERSION     DAZFAH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.209"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1667
                     /locus_tag="ACHJWB_004116"
     CDS             <1..1667
                     /locus_tag="ACHJWB_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4315652.1"
                     /translation="KTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQNKQ
                     DLKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNSFTA
                     AHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGSP
                     QTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSA
                     NENIMQFSDIYLTTRGFLPFAPEADFWGGKHKLPQYEIQMLDWKNLTTDVAAGVGIEN
                     WALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGE
                     SILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            1692..3131
                     /locus_tag="ACHJWB_004117"
     CDS             1692..3131
                     /locus_tag="ACHJWB_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4315653.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(3188..3406)
                     /gene="marB"
                     /locus_tag="ACHJWB_004118"
     CDS             complement(3188..3406)
                     /gene="marB"
                     /locus_tag="ACHJWB_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4315654.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3438..3821)
                     /gene="marA"
                     /locus_tag="ACHJWB_004119"
     CDS             complement(3438..3821)
                     /gene="marA"
                     /locus_tag="ACHJWB_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4315655.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(3841..4275)
                     /gene="marR"
                     /locus_tag="ACHJWB_004120"
     CDS             complement(3841..4275)
                     /gene="marR"
                     /locus_tag="ACHJWB_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4315656.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            4487..5152
                     /gene="marC"
                     /locus_tag="ACHJWB_004121"
     CDS             4487..5152
                     /gene="marC"
                     /locus_tag="ACHJWB_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4315657.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(5177..6367)
                     /gene="ydeA"
                     /locus_tag="ACHJWB_004122"
     CDS             complement(5177..6367)
                     /gene="ydeA"
                     /locus_tag="ACHJWB_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4315658.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6517..7220)
                     /gene="yneK"
                     /locus_tag="ACHJWB_004123"
                     /pseudo
     CDS             complement(6517..7220)
                     /gene="yneK"
                     /locus_tag="ACHJWB_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2003 a         1565 c         1759 g         1893 t
ORIGIN      
        1 taaagacgct taacgtcagc cttttgtctt tttctattat tacagcattg tttccattga
       61 acgcgatggc aacaaaatta accatagagc agcgccttga attgcttgaa aatgaattgt
      121 cgcaaaataa acaagatctg aaagcaacac agaatgaact gggagtatat aaatcccgac
      181 tttcgacctt acaaaaaagc atcacggaaa acaaatataa atcagcctcg cttgctgaaa
      241 tatcagcgac atctcccgtc gctgataaca tcaaaaatga aaacggtgaa cagaactcgt
      301 ttaccgcagc gcatactata aatggatcgc agcaagttgc cgttattgaa agtaaaggcg
      361 acaaaaccac tatcgaaagc gtgaccctga aagatatcag taaatatata aaggatgata
      421 ttgggttcag ctatcagggg tacttccgct caggctgggg tactggtaat cacggctcac
      481 cacaaactta tgcagcgggt tctctgggac gttttggtaa tgagatgagc ggttggtttg
      541 acctgacctt aaatcagcgt gtttataatc aggacggtaa aacggcaaat gcggtcgtta
      601 cctatgatgg caacgtgggt gagcagtata acgatgcctg gtttggtgac agtgccaatg
      661 aaaatatcat gcagttcagc gatatttacc tgacaacgcg aggtttttta cccttcgcac
      721 cagaggcaga cttctggggc ggcaaacata aactcccgca atatgagatc cagatgctgg
      781 actggaaaaa cttaaccacg gatgtcgccg cgggtgtggg gattgaaaac tgggcacttg
      841 gtgtagggct gtttgatatg tccttaagcc gtgatgatgt cgatgtttac tctcgtgatt
      901 tttcgcgtac cagccagatg aataccaatt ctgtggatgt tcgttatcgc aatatcccgt
      961 tatgggatga tgcgacatta tcattaatgg ctaaatattc cgcacctaat aaaacggatc
     1021 aacaacaaga taacgaaaat gacaacagtt attttgaaat gaaagatagc tggatgctga
     1081 cttctgtttt acggcaaaac ctgcaacgcg atacgtttaa tgaatttacg ttacaggttg
     1141 ccaataattc ctatgccagc agttttgcca gtttctcaga tgccagtaat acgatggcgc
     1201 atggtcgcta ttactatggt gatcatacca atgggatcgc ctggcgttta atctctcagg
     1261 gcgagatgta tcttactgac aatattatta tggctaacgc gcttgtctat tctcacggcg
     1321 aagatgttta tagttatgaa agtggtgctc atagtgattt tgacagtatt cgcactgtaa
     1381 taagaccggc ctggatctgg aatacatgga atcagacggg gcttgaatta ggctggttta
     1441 agcaacagaa caaaactcag cagggtgtaa cactaaatga atcggcttat aaaacgacac
     1501 tctggcatgc attgaaagtg ggtgaaagca ttttaggttc acgaccagaa attcgcttct
     1561 atggaacgta tatcaatatt ctggataacg aattatcgaa ttttaagttt aatgagaaca
     1621 gcaaagacga gtttatggcc ggcatccagg cggaagtctg gtggtaatct attttagtta
     1681 aggagttgtg tatgtcagga tttaaaaaag gttttttatg gggcggcgcg gtagccgcgc
     1741 atcagttgga aggtggctgg aatgaaggag gaaaaggcat cagcatcgct gatgtgatga
     1801 ctgctggcgc tcacggggtg ccgcgtgaag tgacagaagg cgttatcgac gggcttaatt
     1861 atcccaatca tgaagcaatt gatttttatc atcgctataa aacagatatt cagttatttg
     1921 ccgagatggg attcaaatgc tttcgaactt ccattgcctg gacacgaatc tttccgcaag
     1981 gtgacgaaca ggagccgaat gaagagggtt tacaatttta tgatgatctg ttcgatgaat
     2041 gccttaagca gggaatggaa cctgtggtga cgctttcaca ttttgagatg ccttatcatc
     2101 tggtgacaaa atatggtggc tggcgaaacc gtaaactgat cgactttttt atccgcttcg
     2161 catcaacggt cttcacgcgc tataaagaaa aagtaaagta ctggatgacg tttaacgaaa
     2221 tcaataatca ggtgaatttc agcgaaagcc tgtgtccatt tactaattcc ggtatcttgt
     2281 attcgccaga ggaagatatc aatgagcgcg aacaaataat gtaccaggcg gtacattacg
     2341 agttagttgc cagtgccctg gcggtacaga ctggaaaatc gatcaatcct gaatttaaca
     2401 ttggctgtat gatcgccatg tgccccatct atcctctgac gtgtgcaccc aacgatatga
     2461 tgatggccac gaaagcgatg catcgtcgtt actggtttac tgatgttcat gctcgtggat
     2521 attatccgca acatatgctg aattactttg ccaggaaagg attcaacctc gatatcacac
     2581 cagaagataa cgcgattctt gccagtggtt gtgtcgactt tatcggcttt agctactaca
     2641 tgtcttttac gacacaattt tcgcccgata acccgcaact ggattatgtt gaaccacgag
     2701 atttggtcag caacccttat atcgatacat ccgaatgggg atggcaaatt gatccggcag
     2761 ggctacgtta ttcactcaac tggttctggg atcatttcca gttgccgctg tttattgtcg
     2821 aaaatggatt tggtgcggtt gaccagagac aagctgacgg cacggtgaac gatcactatc
     2881 gcattgatta ctttgcttcc catattcggg aaatgaaaaa agccgtagtt gaagatggtg
     2941 ttgacttaat tggctacacc ccgtggggct gcattgacct ggtttctgcc ggaacagggg
     3001 aaatgaaaaa gcgctacgga atgatttatg tcgataaaga caacgaaggg aagggaacgc
     3061 tggaacggat acgtaaagcg tcgttttact ggtatcggga tctcatcgcc aacaatggcg
     3121 aaaatatttg agacttgagg aagatattac ggagatgtca ggctctgtga tcagagcctg
     3181 acaataacta catagcgtgt tgattataat agggcacgcc gagcgcatcc gacttatcac
     3241 tgccagtacc catgtgattt aaatcaaacg gtggttgttc ctgcgatggg ggaacaacca
     3301 cgacattggc acaagaagta acaactggct gcgtggtttg ttccgcaacg ccctgcgcgg
     3361 aaaagagaat aagcgcagct gctattgcgg atgaaagtgg tttcatgatt gcctcagtga
     3421 cgttgtcacg ttatcaacta gctgttgtaa tgatttaatg gatgtaaaaa gcgcgattcg
     3481 ccctgcatat tggtcatccg gtatttatgc ggcggaacat caaagtaatt tttgaaggtt
     3541 cgggtcagag tttgttgcga ctcgaagcca tatcgttctg ccagatagag tatcggctcg
     3601 ttactttcct tcagcttttg cgcgatttcc gtcatcttac ggctgcggat gtattggcct
     3661 aatgaatgac cggtttcttt tttaaacatc cgttgcaggt gccatttaga gtaacctgaa
     3721 cgctctgaca ctttctccag tgacagtggc gattccaggt tgtcctcgat ccagtccaaa
     3781 atgctatgaa tggtaatagc gtcagtattg cgtctggaca tcgtcatacc tcttttttgt
     3841 ttacggcagg actttcttaa gcaaatgctc aagtgttgcc acttcgtccg ccgtcaggtt
     3901 ttttgttaat tcttgatgca ggtcctggcc aactaattga tggcattgtt cgcatattgc
     3961 cgcgccgctg gtggtaagtt ttaccagtac gccgcgctta tcattcgggt tcggcaacct
     4021 ttcaacccag cctttacaga ccaggcgatc cagcatacgg gtgagtgctc ccaggtcgac
     4081 cgacaacact tttttcagtt caaccggagt aatacacgcc gcgcagcgga tagagcagag
     4141 caccttaaac tgtgccgcgg taatatccag cggagacaga tactcgttaa gcagacgatc
     4201 tttcttctga ttaaccatat ggattaagcg acccaatgga ataatttcat tgaacagatc
     4261 gctggtactt ttcacattag ttgccctggc aagtaattag ttgcagggga taatattgcc
     4321 caggcaagta taagtcaaat aaatgaattg accgatgcca cgttttgata aatcggttca
     4381 agttttggtc aatgcgatgc aaggttagct aaagagtgac actttatcct tgtttacccc
     4441 ttatactttt cgctgataac ccagatacac aggataacaa ccaccaatgt tagatttgtt
     4501 taaagcaatt ggcctggggc tggtggtgtt actgccgtta gctaacccat taacaactgt
     4561 cgcgttattt cttggcctgg caggcaacat gaacagtgcc gaacgtaatc gtcagtcgtt
     4621 gatggcctcg gtgtacgtat ttgccatcat gatggtggcg tattacgccg ggcaactggt
     4681 gatggataca tttggcattt cgattcccgg tctgcgaatt gcaggcggct taatcgtcgc
     4741 gtttatcggt tttcggatgc tctttccgca acagaaagcg attgattcac cggaggcgaa
     4801 aagcaagtcg gaagagctgg aagatgaacc cagtgccaat attgcctttg tgccgctagc
     4861 aatgccaagt accgctggtc cgggaaccat agcgatgatt attagctcgg cgtccacagt
     4921 gcgtcagagt tcaaccttcg ctgattgggt actgatggtt gcgccgccgc tgatcttttt
     4981 cctggtggcg gttattttgt ggggaagcct acgcagctcc ggcgcaatta tgcggctggt
     5041 gggcaagggt ggcattgaag cgatctcccg tctgatggga ttcctgctgg tatgtatggg
     5101 tgtgcagttt attattaacg gcatcctgga aatcattaaa acgtaccatt aaaaaaggcc
     5161 cgaatgggcc tttcaactat tgcgtctgtt cttcgagtgt cactggccag cggcgaaata
     5221 taatgattga ccaaattaac gcggcaaaag caggcaccgc gcccacataa ccaatcatcg
     5281 acattgacca gtgcaaactc acctgattac ctaccaacgc acccgccccg attccaatat
     5341 taaatatgcc ggagaatagc gccatcgcga cgtcggtagc atctggtgcc agcgccagca
     5401 ctttaacctg cataccaagc ccgatgatca tcatcgcgat cccccagaaa atactcagca
     5461 ccccgaggtg tatttcactg ttcgccgcag gtagcagcag tgccagacac accagcaaca
     5521 gcgcaatcgc cgtactcacc aacgcagacg catactgatt acccagttta ccgaaaatca
     5581 cgctgccaat aatgcccgca ccaccgagta ataacagtaa tgccgtggca aagttggcgc
     5641 tgaatcccgc aatgttttgc acaaaaggct cgatatagct gtatgccgtg taatgggcgg
     5701 tgacaaccac cacagttaac aaataaatgc tcatcaatgc cgggcggcgg aacaatagcg
     5761 ggaggctttt caatgaaccg gaatgctcac tgggcagtaa gggaagtaac ttaatcaggc
     5821 acaaaagggt gataagcgcc ccaataccaa tcgcgaagaa ggtcattcgc caaccgaaat
     5881 actggcccac aatgcgcccg agaggtaaac ctaagaccat cgccagtgct gtaccggtgg
     5941 caattaaact caaggcctgt gcccgcttcc cggccggagc catacggatc gccagagacg
     6001 ccgtaatcga ccagaaaatc gcatgtgcaa aagccacacc aatgcgactg atcaccagaa
     6061 cggtaaagct ccacgataaa aacgacagta cgtggctggc aataaacacc acaaacaggc
     6121 agatcagtaa tttgcgccgt tcaacctggc tggtcattaa cataaaaggc aatgacatta
     6181 gcgccactac ccatgcataa atggtcaaca taatgccgac ctgagcggtt tgcatgtgaa
     6241 aactttgcgc aatgtcagag agcaggccaa cagggacaaa ttcggtggtg ttgaagatga
     6301 aggcggcgac tgccagcgta acgacccgta gccacgccac tttgcgggaa acagtgtttg
     6361 ttgtcataac agattatcgg gtgagttgcg aaaaagtgat gaaatgatct taaaacggtt
     6421 aatgatgtga atacaatgca tttgtgatct ggatcgcgtt ttccgtttct ctgacgctga
     6481 ttctgttccg cgtcagagag tcggtaacat cacggtttaa tgacgttcag ggaggtctgg
     6541 cgttttggcg gttgcgggta taggtggtct gacaggggag cgaggccttt ttttaccagg
     6601 tcttcgtgag taacttttgg cggcgtttca ttttttacaa acagtttgag ggtcctgcgc
     6661 gccatatgtt tgataatttt atcatcactt aatgatgctg gcatcaacac gtcattcaga
     6721 tcactttcat tgggattcat aaattggtga tagtgcaata acatattggc gaaaaataaa
     6781 aaaaagtcct gaaaatgagg agattcttgt tctgctaaaa gggataaaaa gcagatgcag
     6841 acttcgggta gtacatgttt gttagaacct aatgaatcca tcaggctttg caataaatcc
     6901 tcttttaatt cgccgtctgt tatgtcgaga atggcggtga tcaagttagg atctttattg
     6961 acgaaccatc tgtgaacctc agagttactc cataatcggg ggagtatgac aaaattaccg
     7021 cgagagataa aattcaggac aagcaaatca aactggcgta agtaatctgc agcggtatgg
     7081 cgatccggag gcaggggttc aatatgtggt gtatgcgctc ttttgatggg aacgccaagg
     7141 ttgatcattg gaaattgttc aataaaatca ttcagaaaca gaaatagcac acgctcgacg
     7201 ccggtctgaa atacgcgccc
//
LOCUS       DAZFAH010000208         7211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFAH010000208 DAZFAH010000000
VERSION     DAZFAH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.210"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..185
                     /locus_tag="ACHJWB_004124"
     CDS             <1..185
                     /locus_tag="ACHJWB_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315659.1"
                     /translation="IRQILTRRFRLYAYDCNDRPNYQIQALFTASPVPVTSEELVKAV
                     HFYEQLKRENPPTSGN"
     gene            975..2045
                     /locus_tag="ACHJWB_004125"
                     /pseudo
     CDS             975..2045
                     /locus_tag="ACHJWB_004125"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            2118..2267
                     /gene="ydfW"
                     /locus_tag="ACHJWB_004126"
     CDS             2118..2267
                     /gene="ydfW"
                     /locus_tag="ACHJWB_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO4315660.1"
                     /translation="MDNARIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKKD"
     gene            complement(2712..3044)
                     /gene="flxA"
                     /locus_tag="ACHJWB_004127"
     CDS             complement(2712..3044)
                     /gene="flxA"
                     /locus_tag="ACHJWB_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4315661.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(3247..3552)
                     /gene="ydfV"
                     /locus_tag="ACHJWB_004128"
     CDS             complement(3247..3552)
                     /gene="ydfV"
                     /locus_tag="ACHJWB_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO4315662.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            3577..3816
                     /gene="relB"
                     /locus_tag="ACHJWB_004129"
     CDS             3577..3816
                     /gene="relB"
                     /locus_tag="ACHJWB_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO4315663.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            3816..4103
                     /gene="relE"
                     /locus_tag="ACHJWB_004130"
     CDS             3816..4103
                     /gene="relE"
                     /locus_tag="ACHJWB_004130"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO4315664.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            4175..4330
                     /gene="hokD"
                     /locus_tag="ACHJWB_004131"
     CDS             4175..4330
                     /gene="hokD"
                     /locus_tag="ACHJWB_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4315665.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            4547..4798
                     /gene="rem"
                     /locus_tag="ACHJWB_004132"
     CDS             4547..4798
                     /gene="rem"
                     /locus_tag="ACHJWB_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO4315666.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            4865..5143
                     /locus_tag="ACHJWB_004133"
     CDS             4865..5143
                     /locus_tag="ACHJWB_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315667.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            5145..6194
                     /gene="ydfU"
                     /locus_tag="ACHJWB_004134"
     CDS             5145..6194
                     /gene="ydfU"
                     /locus_tag="ACHJWB_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4315668.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            6208..6960
                     /gene="ydfT"
                     /locus_tag="ACHJWB_004135"
     CDS             6208..6960
                     /gene="ydfT"
                     /locus_tag="ACHJWB_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4315669.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
BASE COUNT         1923 a         1547 c         1821 g         1920 t
ORIGIN      
        1 taattcgtca gattctgacc agacggttca ggctgtacgc ttacgattgt aacgacaggc
       61 cgaactatca aattcaggcc ttattcactg cctcaccagt cccagttaca tcagaagaac
      121 tggttaaagc tgtgcacttt tatgaacaac taaaacgcga aaatccacca acatccggca
      181 actagattaa tggattaact atgtcggtta aacgaccagc caactgaaaa agcggaaacc
      241 tgattacagg ttgccagata aggcaatgag ctacctggcg cggaacggac tgataagtat
      301 ggggaatgtt ttacgatgaa cctttagact aaagagtttg taacgctatg taagtgattt
      361 tttctggttt agatatttat atgtccggcc aaattgaggt gtgtttaaat gtaattgcac
      421 attgattgta ggaggaataa tgaaaaacgc attgcagttt ttgtttgttg cgttctggtt
      481 gttcgcatca tgtatgccca tcatcttcac agcaaggtat atggaaaaag ttgatgtttt
      541 gatattaata tttggatata taaatgccct ttttttaggg gtgttcatgg cggtcatgtg
      601 cattgaatac tggcggtaaa tacagcgaac tccattggtt tagttggata tttactgtgc
      661 tggacaaaaa cggtttgcgg ggaaatctta gttaagtaga atgactgcgg gtgcttgagg
      721 ctatctgcct cgggcatgga caccaacggc agatagagaa aagccccagt taacattacg
      781 cgtcctgcaa gacgcttaac attaatctga ggccaatttc atgctagaca catgtaggtt
      841 agcctcttac gtgccgaaag gcacggagaa gcaggctatt gttaacacca agctgtaatg
      901 tcccctttga accattctaa aatgtcccca gacaattctc tgggggattt ttcatgatca
      961 aagagactgt tacgatgagt cataaggaac tccaccgact tcagattatt caggaacaag
     1021 ctgcggcacg cattggcatt tctattcggc aggttaaacg tctggtgcaa cggtatagaa
     1081 atgaagggcc ttctggtctg gtttcccacc gacgtggaaa gcgtcctaat aattcctttt
     1141 ctactgaatt cagagcaaca gtaatttcac tcctcaaagg ccgttacgct gattttggac
     1201 ctacgtttgc gtgcgaaaaa ttgcgcgaga tacacggttt atctttatcc gttgaaactc
     1261 tcagaaagtg gatgatagaa gaggggttat ggcgtgaacg ccgtcgtaaa attgcccgta
     1321 tatatcaacg ccgcatgcga cgaccatctt acggtgaact gatccagatt gatggctcac
     1381 ctcatgactg gtttgaaaat cgaggcccca gatgtacact gatcgttttc attgatgatg
     1441 ccaccagtgc gttgatggcg ttgcgttttg tgcctgctga aacaacccgg gcttacatgg
     1501 aaaccctccg gggttacctt aatgatcatg gcgtaccgct cgctctctac tctgatagac
     1561 acagtatatt cagggtaaat aacccagagc gggaaggtga gctgacccag ttcactcgtg
     1621 cgataaagac actgggcatc gagccaatcc atgccaacag cccgcaggca aaagggcggg
     1681 tagagcgcgc caatcagaca ctacaggaca ggctggtcaa agaaatgcgg cttcagaata
     1741 tcagtgatat tgaaacagca aatgcatggt tgccgacctt tattgaagcc tataacaacc
     1801 ggttcgctac gtcgcctcgt actactgata atgctcatct tgatgtgcac cattctgaag
     1861 aggaactggg ttatatcttc agcctacagg cgaagcgcgt tctgtctaaa aatctcactt
     1921 tccagtacaa aagcagtgcg tttcaggtac gcagtgaggg ccggggatat cgacttaggc
     1981 attcggttgt tactgtatgc gagaactttg acggtgaaat taacgttctg tatgacggga
     2041 aagcgctggg ctgggaaaag tatgttgatg gcccggagcc tataccactg gatgatgaaa
     2101 agagtgtcca tgaacgagtg gataatgccc gtattgattt acgctcaaaa tactatgtta
     2161 aacctaaagc tgaccatccc tggcttacgc gccgaacgca aagtcatcag caagttaagc
     2221 ccccgaagtt acctaaaaag aagcctgatc ccgataaaaa agattgaaac caagatcgat
     2281 tcggttgagt gcatatccat tcatagggta gattcttaag tcgcgtttct ggtgttcatt
     2341 ttcgggtggt ttgttacttg ttttaccggg gatatgccag aaacgcgctg agtcagtctg
     2401 ggcggtgcgc gtaatgaggc gttaaggcgt tatggtaaat agcctatgct aatgtccgct
     2461 aagagcaaga agcggaagtt ggcagttttg tggactgtcc ccacaaaagt gactacagaa
     2521 atagttgcaa ttcataattg atcatgggtt gtcagttaaa ctcgtggcga tttaaataga
     2581 ctaattggga gtgcgtccat tacttatatc ttgtaatgtt aactatcaga aatgatacaa
     2641 agataatatg tctttaaaga aaaggctgat ggcgaaaagt ggcccgatga gggccacaat
     2701 acggctgtca cttagacgta aatatcaatg gtgccagcgg tatttgtatc gtcttttttc
     2761 tcttcttttt tatcaggctg aactgtcgcg tcttcattct ttttctctgc ctgctgcctt
     2821 aacaactgct ccagttgagc ccagaggctt tcaatttgct tctgtaccaa tgcagccatt
     2881 tcttttttct gctgtgtcgt catcccctct tccgatgaga ttttcccaag cttttcagtc
     2941 agcacctgaa tttgtcttgt gattttggct atttctgatg ttccttccgg ggcggagttg
     3001 tttgaaataa cggttgaggt atttccctga attgtgacag acatagattt ctccttttaa
     3061 aaaagcacta tcggcatgca caaaaaaatc tttaatcgta tttcttgtgt cattaattgt
     3121 ttgatgttca gattgttttc ctcgcgggct ggcgcgcctc agaaagtaaa gcttgttgac
     3181 aggggtaaac gttcggcaat aattttctgc cgcatgcggg tgttgcataa aacgtgttac
     3241 gttcctttat cgacaggtca ggtcaccgct cacccgccga cgagaaagca acactgacat
     3301 gctaaagcaa aaaatagatg aataagttga gttgtgcata tgtagcctga ccgtcacaaa
     3361 gtatatggtg tctgtaccag taagatgatg gccggactct ttaaaaacga gctgacctgc
     3421 acaatacagg atggacttag caatggctgc tcctggcaca aagcggacag tgatcaccgt
     3481 tcttacgact actttctgac ttccttcgtg acttgcccta agcatgttgt agtgcgatac
     3541 ttgtaatgac atttgtaatt acaagaggtg taagacatgg gtagcattaa cctgcgtatt
     3601 gacgatgaac ttaaagcgcg ttcttacgcc gcgcttgaaa aaatgggtgt aactccttct
     3661 gaagcgcttc gtctcatgct cgagtatatc gctgacaatg aacgcttgcc gttcaaacag
     3721 acactcctga gtgatgaaga tgctgaactt gtggagatag tgaaagaacg gcttcgtaat
     3781 cctaagccag tacgtgtgac gctggatgaa ctctgatggc gtattttctg gattttgacg
     3841 agcgggcact aaaggaatgg cgaaagctgg gctcgacggt acgtgaacag ttgaaaaaga
     3901 agctggttga agtacttgag tcaccccgga ttgaagcaaa caagctccgt ggtatgcctg
     3961 attgttacaa gattaagctc cggtcttcag gctatcgcct tgtataccag gttatagacg
     4021 agaaagttgt cgttttcgtg atttctgttg ggaaaagaga acgctcggaa gtatatagcg
     4081 aggcggtcaa acgcattctc tgaaccaaag catgacatct ctgtttcgca ccgaaggtga
     4141 cacttctgct ttgcgttgac aggagaagca ggctatgaag cagcaaaagg cgatgttaat
     4201 cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga ggaaagacct
     4261 ctgcgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcacag cttacgaacc
     4321 tgaggagtaa gagacccggc gggggagaaa tccctcgcca cctctgatgt ggcaggcatc
     4381 ctcaacgcac ccgcacttaa cccgcttcgg cgggtttttg tttttatttt caacgcgttt
     4441 gaagttctgg acggtgccgg aatagaatca aaaatactta agtagcgcgc agggataaga
     4501 gggatggtcc cttaaagggg agagctaatt atccggaagg attctgatga tgaacatcga
     4561 agaactgcgt aaaatttttt gtgaagatgg cctctatgct gtgtgcgttg aaaatggaaa
     4621 tcttgttagt cattaccgca ttatgtgttt gcgaaagaat ggggctgcgt taattaattt
     4681 tgtggatgct cgggtcacgg acggatttat cttgcgcgaa ggtgagtttg tcacttcatt
     4741 acaggcattg aaagagatcg gaataaaagc tggcttttct gctttttcag gagaataaac
     4801 tcatctacaa tcttgcgcgg ggctgaactc ccgctgagta acaccgtgcc accggagaaa
     4861 accgatggca cgcaacgcaa aatattacaa ttctgataat tcgcccgttc ttgcctgcac
     4921 gcacgggcgg tattctcacg cattcaagtc tgaatggttc cagcaccctc catgcactgc
     4981 agaacaggcc gaatggctga ttcattctta ccgcaggcgc gggttcgagg ttaagaaagc
     5041 tctcagtctc gactatcggc actggataat ctctgtcagg ctgccttatt ccgaacgccc
     5101 accacgtgcg tcccgcactt tccagcaacg gatctggagg taacgtgcgg gtattactta
     5161 gacctgttct ggtgcctgag cttgggctgg tggtccttaa gccgggccgt gaatccatac
     5221 agatatttca taatcctcga gtgctggtgg aaccggaacc aaaaagcatg cgtaatctgc
     5281 catccggagt cgttcctgcc gttcgccagc cgctggcgga agacaaaaca ttgctgccgt
     5341 tttttagtaa cgaacgggtg attcgtgctg ctggcggcgt tggcgcattg tccgactggc
     5401 tattacgtca tgttacatcc tgccagtggc ctaatggcga ttaccatcac actgaaacag
     5461 tcattcaccg ttatggtacc ggcgcaatgg tgttgtgctg gcactgcgac aaccaactgc
     5521 gtgaccagac atcggaatca ctggagctgc ttgctcaaca aaatctgaca gcatgggtga
     5581 ttgacgtcat ccgtcacgca ataagcggta cgcaggagcg ggaattatct ttggctgaat
     5641 tatcctggtg ggcggtctgc aatcaggtgg tggatgcact acctgaggct gtatcgcgtc
     5701 gttcgctggg attaccagcg gaaaaaatct gctcggtgta ccgcgaaagc gacatcgtac
     5761 cgggagagca gaccgccacc agcatattga aacaacgcac aaaaaatctt gcaccgttgc
     5821 cttacgccca ccagcaacaa aaatcaccac aggaaaagac ggtggtaagc atcaccgttg
     5881 atccagagtc tccggaatct ttcatgaagc tgcctaaacg tcgccgctgg gttaaggaga
     5941 aatacacacg ttgggttaag acacagccgt gtgcttgctg cggtatgcca gccgacgatc
     6001 cgcatcatct gattggtcac gggcagggcg gaatgggaac aaaagcacat gatctctttg
     6061 tgttgccttt gtgcagaaag catcacaacg agctgcatac ggatacagtg gcatttgaag
     6121 ataagtatgg ctcccaactg gagctgatat ttcgttttat cgatcgcgcg ctggcaattg
     6181 gcgtactggc gtaagtggag aacgagcatg aaccttgaag ccttaccaaa atattactcc
     6241 ccaaaatctc caaaattgag cgatgacgct ccagcgacag gcaccggttg tttaacaatt
     6301 acggatgtaa tggcagcgca ggggatggtg cagtcgaaag caccacttgg gttggcctta
     6361 tttctggcaa aagttggtgt tcaggaccct cagtttgcga ttgaaggcct gctaaattac
     6421 gcgatggcac tggataaccc gacattgaac aaattgagtg aagaaatccg gttacagatt
     6481 attccttacc tcgtgagttt tgcctttgct gattactcca ggtctgcggc aagtaaggct
     6541 cgctgtgagc attgttcagg tacgggattt tataatgtat tgcgcgaagt ggtgaaacac
     6601 tacagacgcg gggaatctgt aatcaaggaa gaatgggtga aggaactatg tcagcattgc
     6661 catggtaagg gcgaagccag cacagcgtgc agagggtgta agggtaaagg gattgttctg
     6721 gatgaaaaaa gaacccggtt tcatggcgta ccggtatata agatttgtgg gcgttgtaat
     6781 ggaaaccggt ttagtcgttt accgaccacg ctggcacgac gtcatgtcca gaagctggta
     6841 ccagacctga ccgattatca gtggtataag gggtatgcgg acgtcattgg taaactggta
     6901 acaaagtgct ggcaggaaga agcatacgcg gaagcgcaat tgaggaaggt gacgagataa
     6961 atgatttttg ctgaagatgg cgacatgatg tttgcatttt tcaaaaaata tggataaaat
     7021 tttttcaacg atgggctttg tatacccgac gttaagaaaa agtagaaaac ccgctgatga
     7081 gcgggttttg tgctttaaat ggggcaatgg taatgttgaa tctcatcccg ggactcatgt
     7141 ctgttaactt attatttagc tggtgacttg gttatttgcc tgatgtttaa aatgttttct
     7201 tccagtacaa t
//
LOCUS       DAZFAH010000209         7080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFAH010000209 DAZFAH010000000
VERSION     DAZFAH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.211"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            512..1501
                     /gene="araF"
                     /locus_tag="ACHJWB_004136"
     CDS             512..1501
                     /gene="araF"
                     /locus_tag="ACHJWB_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4315670.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1571..3085
                     /gene="araG"
                     /locus_tag="ACHJWB_004137"
     CDS             1571..3085
                     /gene="araG"
                     /locus_tag="ACHJWB_004137"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4315671.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3100..4086
                     /gene="araH"
                     /locus_tag="ACHJWB_004138"
     CDS             3100..4086
                     /gene="araH"
                     /locus_tag="ACHJWB_004138"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4315672.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4253..5053
                     /gene="otsB"
                     /locus_tag="ACHJWB_004139"
     CDS             4253..5053
                     /gene="otsB"
                     /locus_tag="ACHJWB_004139"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4315673.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5028..6452
                     /gene="otsA"
                     /locus_tag="ACHJWB_004140"
     CDS             5028..6452
                     /gene="otsA"
                     /locus_tag="ACHJWB_004140"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4315674.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6459..6887)
                     /gene="uspC"
                     /locus_tag="ACHJWB_004141"
     CDS             complement(6459..6887)
                     /gene="uspC"
                     /locus_tag="ACHJWB_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4315675.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1801 a         1645 c         1825 g         1809 t
ORIGIN      
        1 gatgaagtga tgattttata aaacgttttt tcatttttgc gagatggctc tcattatacg
       61 tgttctgtta attaatgagc acagtgataa ttaatgacaa aatgaatgac gtgcattttc
      121 cacatctttg agttgcggtt attacaccat ttcaaaaact caacgccagg taatgcggcc
      181 tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa ggatttccag
      241 gctaatctta tggattaatc tgctgtgcat tcgacaattt gtctgacaaa ttggctttcc
      301 cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg atgtgatatt
      361 gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacggtca cttatctttt
      421 agttaaaagg taatgctttg ttttccgatt aatttaacga atgtcattcg tttttgccct
      481 acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag ccctggcagc
      541 cattggtctg gcagccgtta tgtcacaatc cgctatggcg gagaacctga agctcggttt
      601 tctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg ccgataaagc
      661 cgggaaggat ttagggtttg aggttattaa gattgccgtg ccggatggcg aaaaaacatt
      721 gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt gtactccgga
      781 ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga aagtcattgc
      841 cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc cgctggtgat
      901 gatggcggcg actaaaattg gcgaacgtca gggtcaggaa ctgtataaag agatgcagaa
      961 acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg aactggatac
     1021 ggcacgtcgc cgtacaacgg gttctatgga tgcgctgaaa gcggccggat tcccggaaaa
     1081 acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg acgctgccaa
     1141 ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta tgaacgacag
     1201 caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagctg ccgatatcat
     1261 cggcattggc attaacggtg tggatgcggt gagcgaactg tctaaagcac aggcaaccgg
     1321 cttctacggt tccctgttgc caagcccgga cgtacatggt tataagtcca gcgaaatgct
     1381 ttacaactgg gtagcaaaag gtgttgaacc gaccaaattc accgaagtta ccgacgtggt
     1441 gctgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag gcggtaagta
     1501 atttgccgga aaaattcccc tctgcatgat gcagaggggg tgtgaacgac cagtgattca
     1561 cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg gtaaaacgtt
     1621 tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc aggttcatgc
     1681 gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca gcggcaacta
     1741 tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt ccgacacgac
     1801 cgcagcactt aacgcgggtg tggcgattat ttaccaggaa ctgcatctcg tgccggaaat
     1861 gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca ttgtgaatcg
     1921 ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg atattgaccc
     1981 ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa tcgccaaagc
     2041 gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca accagctccc tttctgcccg
     2101 cgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggc gggtgatctt
     2161 atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca ctgtctttaa
     2221 agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg acgcgctggt
     2281 gcaggcgatg gtcgggcgcg acattggcga tatctacggc tggcaacctc gtagttatgg
     2341 cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc caataagtct
     2401 ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg ggcgtagcga
     2461 attaatgaaa ggcttgtttg gcgggacgca aatcaccgcc ggtcaggttt atatcgacca
     2521 acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga tgctctgccc
     2581 ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca atatcaacat
     2641 cagtgccaga cgcaaacatg tgctcggcgg ttgtgtaatc aacaacggtt gggaagaaaa
     2701 caatgccgat cagcacattc gttcgctcaa catcaaaacg ccgggcgctg agcaactgat
     2761 catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt tatcggaaga
     2821 gatgaaggtc attttgctgg atgaacctac gcgcggcatt gatgttggtg ctaagcacga
     2881 aatatataac gtaatttatg cgctggcggc gcagggtgtg gcggtgctgt ttgcctccag
     2941 cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg aaggtgaaat
     3001 cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc ttgcgatgcc
     3061 taaagtcagc caggcagttg cctgagtaag gagagtatga tgtcttctgt ttctacatcg
     3121 ggttctggcg cacctaagtc gtcattcagc ttcgggcgta tctgggatca gtacggcatg
     3181 ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt ttgtcccaaa ttttgccacc
     3241 ttcattaata tgaaagggtt gggcctggca atttccatgt cggggatggt ggcgtgtggc
     3301 atgttgttct gccttgcttc cggtgacttt gacctttctg tcgcctccgt aattgcctgt
     3361 gcgggtgtca ccacggcggt ggttatcaac ctgactgaaa gcctgtggat tggcgtggca
     3421 gcggggttgc tgcttggcat tctctgtggc ctggtcaatg gctttgttat cgccaaactg
     3481 aaaataaatg ctctgatcac aacactggca acgatgcaga ttgttcgagg tctggcgtac
     3541 atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc ccttggttac
     3601 gctaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct gattatcttt
     3661 ggtttgttgc tgaataaaac cacctttggt cgtaacaccc tggcgattgg cgggaacgaa
     3721 gaggctgcgc gtctggcggg tgtaccggtt gttcgcacca aaattattat ctttgttctc
     3781 tctggcctgg tatctgcgat agccggaatt attctggctt cacgtatgac cagtgggcag
     3841 ccaatgacgt cgattggtta tgagcttatt gttatctccg cctgcgtttt aggtggcgtt
     3901 tctctgaaag gtggcatcgg aaaaatctca tatgtggtgg cgggtatctt aattttaggc
     3961 accgtggaaa acgccatgaa cctgcttaat atttctcctt tcgcgcagta cgtggttcgc
     4021 ggcttaatcc tgctggcagc ggtgatcttc gaccgttaca agcaaaaagc gaaacgcact
     4081 gtctgatgct tttttccaca acaatttaac gttttttccc accacagcca gccgccacaa
     4141 cggttggctg ttcttcattg caaatggcga cccccgtcac actgtctata cttacatgtc
     4201 tgtaaagcgc gttctgcgca aaacaataag aaaagagaag gaggagaacc gggtgacaga
     4261 accgttaacc gaaacccctg aactatccgc gaaatatgcc tggttttttg atctcgatgg
     4321 aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtt gtgcctgaca atattctgca
     4381 aggacttcag ctactggcaa ccgcaagtga tggtgcattg gcattgatat cagggcgctc
     4441 aatggtggag cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg gcgtgcatgg
     4501 ggcagagcgc cgtgacatca atggtaaaac acatatcgtt catctgccgg atgcgattgc
     4561 gcgtgatatt agcgtgcaac tgcatacagt catcgctcag tatcccggcg cggagctgga
     4621 ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct ccgcagcatg aagacgcatt
     4681 aatgacatta gcgcaacgta ttactcagat ctggccacaa atggcgttac agcagggaaa
     4741 gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt gaggcaattg cagcttttat
     4801 gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg ggcgatgatt taaccgatga
     4861 atctggcttc gcagtcgtta accgactggg cggaatgtca gtaaaaattg gcacaggtgc
     4921 aactcaggca tcatggcgac tggcgggtgt gccggatgtc tggagctggc ttgaaatgat
     4981 aaccaccgca ttacaacaaa aaagagaaaa taacaggagt gatgactatg agtcgtttag
     5041 tcgtagtatc taaccggatt gcaccaccag acgagcacgc cgccagtgcc ggtggccttg
     5101 ccgttggcat actgggggca ctgaaagccg caggcggact gtggtttggc tggagtggtg
     5161 aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa aggtaacatt acgtgggcct
     5221 cttttaacct cagcgaacag gaccttgacg aatactacaa ccaattctcc aatgccgttc
     5281 tctggcccgc ttttcattat cggctcgatc tggtgcaatt tcagcgtcct gcctgggacg
     5341 gctatctacg cgtaaatgcg ttgctagcag ataaattact gccgctgttg caagacgatg
     5401 acattatctg gatccacgat tatcacctgt tgccatttgc gcatgaatta cgcaaacggg
     5461 gagtgaataa tcgcattggt ttctttctgc atattccttt cccgacaccg gaaatcttca
     5521 acgcgctgcc gacatatgac accttgcttg aacagctttg tgattatgat ttgctgggtt
     5581 tccagacaga aaacgatcgt ctggcgttcc tggattgcct ttctaacctg acccgcgtca
     5641 cgacacgtag cgcaaaaagc catacagcct ggggcaaagc atttcgaaca gaagtctacc
     5701 cgatcggcat tgaaccgaaa gaaatagcca aacaggctgc cgggccactg ccgccaaaac
     5761 tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc gaacggctgg
     5821 attattccaa aggtttgcca gagcgttttc tcgcctatga agcgttgctg gaaaaatatc
     5881 cgcagcatca tggtaaaatt cgttataccc agattgcacc aacgtcgcgt ggtgatgtgc
     5941 aagcctatca ggatattcgt catcagctcg aaaatgaagc tggacgaatt aatggtaaat
     6001 acgggcaatt aggctggacg ccgctttatt atttgaatca gcattttgac cgtaaattac
     6061 tgatgaaaat attccgctac tctgacgtgg gcttagtgac gccactgcgt gacgggatga
     6121 acctggtagc aaaagagtat gttgctgctc aggacccagc caacccgggc gttcttgttc
     6181 tttcgcaatt tgcgggagcg gcaaacgagt taacgtcggc gttaattgtt aacccctacg
     6241 atcgtgacga agttgcagct gcgctggatc gtgcattgac tatgtcgctg gcggaacgta
     6301 tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac cactggcagg
     6361 agtgcttcat tagcgaccta aagcagatag ttccgcgaag cgcggaaagc cagcagcgcg
     6421 ataaagttgc tacctttcca aagcttgcat aggagcggtt aatctcccgt aagtggaact
     6481 aacagcacat cgacctcact tgaggcaatc actcttttcg ccgagcagga tgctcgcgaa
     6541 aagaaactgt gattgtgatt accgcaaatc accaaatcga aatgatgttt gtgacatact
     6601 tccagaatat gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac gggatacccc
     6661 gcatcctgaa ttaacttatc aagaaagctt tgcgtttctt cctgcatcac gctgcgtaaa
     6721 tcttcaagca tcggcgcagc taattgattg tacatttccg ggtcggaagc gagagtaatt
     6781 aaactgatgt gtccctttac tggcctggcg atagataccg cttttgccag cagttgctga
     6841 ctttccggtg taaccgcaac agcgacaaga atattgctat agctcataac ctgttcctta
     6901 ttctgtgaac ttcaggtgac attaaagctt tactcctaat ggagaaacga cgcaatccca
     6961 actcagtcaa acaggtaaat attagtctga ttattagtaa agacaattaa tcaatcactc
     7021 ccggtgatca tatgatttca actgtaatta taagaaaata atgtactgat tccccgtatt
//
LOCUS       DAZFAH010000210         7016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFAH010000210 DAZFAH010000000
VERSION     DAZFAH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.212"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..803
                     /gene="hlyE"
                     /locus_tag="ACHJWB_004142"
     CDS             <1..803
                     /gene="hlyE"
                     /locus_tag="ACHJWB_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO4315676.1"
                     /translation="QTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQ
                     TVYEWCGVATQLLAAYIFLFDEYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQ
                     SFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYS
                     IAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKT
                     ETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1010..1456)
                     /gene="ycgN"
                     /locus_tag="ACHJWB_004143"
     CDS             complement(1010..1456)
                     /gene="ycgN"
                     /locus_tag="ACHJWB_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4315677.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1548..2207)
                     /gene="ycgM"
                     /locus_tag="ACHJWB_004144"
     CDS             complement(1548..2207)
                     /gene="ycgM"
                     /locus_tag="ACHJWB_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4315678.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2279..2572)
                     /locus_tag="ACHJWB_004145"
     CDS             complement(2279..2572)
                     /locus_tag="ACHJWB_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4315679.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2814..3215
                     /gene="pliG"
                     /locus_tag="ACHJWB_004146"
     CDS             2814..3215
                     /gene="pliG"
                     /locus_tag="ACHJWB_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4315680.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3335..3703)
                     /gene="ycgJ"
                     /locus_tag="ACHJWB_004147"
     CDS             complement(3335..3703)
                     /gene="ycgJ"
                     /locus_tag="ACHJWB_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4315681.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4223..4918
                     /gene="minC"
                     /locus_tag="ACHJWB_004148"
     CDS             4223..4918
                     /gene="minC"
                     /locus_tag="ACHJWB_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4315682.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4942..5754
                     /gene="minD"
                     /locus_tag="ACHJWB_004149"
     CDS             4942..5754
                     /gene="minD"
                     /locus_tag="ACHJWB_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4315683.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5758..6024
                     /gene="minE"
                     /locus_tag="ACHJWB_004150"
     CDS             5758..6024
                     /gene="minE"
                     /locus_tag="ACHJWB_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4315684.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6396..6739)
                     /locus_tag="ACHJWB_004151"
                     /pseudo
     CDS             complement(6396..6739)
                     /locus_tag="ACHJWB_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6774..6893)
                     /locus_tag="ACHJWB_004152"
     CDS             complement(6774..6893)
                     /locus_tag="ACHJWB_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4315685.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAV"
     gene            complement(6854..>7016)
                     /gene="ymgJ"
                     /locus_tag="ACHJWB_004153"
     CDS             complement(6854..>7016)
                     /gene="ymgJ"
                     /locus_tag="ACHJWB_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO4315686.1"
                     /translation="TITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLF
                     LMRDIIISS"
BASE COUNT         1994 a         1671 c         1555 g         1796 t
ORIGIN      
        1 ggcagacctt tgatgaaacc ataaaagagt taagtcgctt taaacaggag tattcacagg
       61 cagcctccgt tttagtcggc gatattaaaa ccttacttat ggatagccag gataagtatt
      121 ttgaagcaac ccaaacagtg tatgaatggt gtggtgttgc gacgcaattg ctcgcagcgt
      181 atatttttct atttgatgag tacaatgaga agaaagcatc cgcccagaaa gacattctca
      241 ttaaggtact ggatgacggc atcacgaagc tgaatgaagc gcaaaaatcc ctgctggtaa
      301 gctcacaaag tttcaacaac gcttccggga aactgctggc gttagatagc cagttaacca
      361 atgatttttc agaaaaaagc agctatttcc agtcacaggt agataaaatc aggaaggaag
      421 cgtatgccgg tgccgcagcc ggtgtcgtcg ccggtccatt tggattaatc atttcctatt
      481 ctattgctgc gggcgtagtt gaagggaaac tgattccaga attgaagaac aagttaaaat
      541 ctgtgcagaa tttctttacc accctgtcta acacggttaa acaagcgaat aaagatatcg
      601 atgccgccaa attgaaatta accaccgaaa tagccgccat cggggagata aaaacggaaa
      661 ctgaaacaac cagattctat gttgattatg atgatttaat gctttctttg ctaaaagaag
      721 cggcaaaaaa aatgattaac acctgtaatg agtatcagaa aagacacggt aaaaagacac
      781 tctttgaggt acctgaagtc tgataagcga ttattctctc catgtactca aggtataagg
      841 tttatcacat ttaaaaacac agacgttttt taaagattga gagttacctg ttgataataa
      901 ggtatcgggt aactctcatt atgacagagc gctttcaaaa acagggctac gaaatggcga
      961 gcatccagac gtatattaag aaagccccca tcaacatcta ttaaatttat cactgcgccc
     1021 agtcaggttt atttaagata tgatcctgcc agtcaatcac ttctgattct ttcactgcga
     1081 tatgacgcac agagatacgt tcaccatgca ttgccgcttt cgaaccagta agtagcggat
     1141 gccacgcagg taaatcttta ccttccgcca gcaaacgata agcgcaggtc attggcagcc
     1201 attcgaatgt tggcagattt tcacgggtta atttaatgca gtcgggttca aactcgaaac
     1261 gacgttcgta gttccgacat tgacaggttt taatattgag ctggcgacag gcgacgttag
     1321 tgaagtagat ttcgtcggtg tcttcatcca tcagtttatg caggcaacac tgaccgcaac
     1381 catcacacaa cgactcccat tccgcatcgc tcatttcgtc cagggtttta ctttgccaga
     1441 aaggtacatc gctcatcagg tgttccgcca ttacgttaaa acgcacctta taaccagtct
     1501 ggcacagcga tgcaagtttt gccgccgctt tcaggcggca aaaagtatta caaaacgcga
     1561 gttgtcaaag aatgcccatc gaaagtgact gtcagctcat caccgctttg caacgggccg
     1621 acgccatcag gcgtgcctgt caacacaacg tcaccggcct tgagggtaaa aaacttgctc
     1681 atataagcga tcaacggaac gattttatgg atcatgtccg cagtcgtacc ttgctggcgt
     1741 tgttcgccgt ttacgctcag gcccagcgtt gtattttgtg gatcgccggt aaattccgcc
     1801 gcggggataa acccggaaag cggacaagag ttatcaaacg ctttagcctt ttcccacggc
     1861 tgcccggctt tcttcatttt tccctgaaca tcacgcaatg tcagatcgag cgccacgccg
     1921 taaccggcaa tggctttgcg gacatgctct tccgttgcct gacgcagcgt cgcgccaatc
     1981 aacaccgcca gttcgacttc atgatgaact gaaccgaaat cggatgggat cgccaatggc
     2041 tgccgcagat cgcacagtgc cgtttctggt ttaataaaca gcactggctc ttcgggcact
     2101 gcgctgccca tctctttaat atgtttggca tagttactgc caacacagac tactttactc
     2161 accggataat ccagcagcgc accttgccag ttgtgatgtt gatacataac gttcccctca
     2221 ccttatcgtg gatatgaaaa cccgcgccga caaagataat gccaccgccg gatatcggtt
     2281 atttgttagt gtcgtctgtt ttctgcccca tgacggaaag atgttgcttc agcaaatctt
     2341 cgggtggtgg cggtaactgc aaatagtaac cttgctcggt taatgccagt ttaacctttt
     2401 caatatcagc attgaccagt ttcttccgcc catccaacgg cagaatcatc gctaactgag
     2461 gctgaccaaa acctttcatc agttcctcag gaacacgcga aaaatcgtct tttttttcga
     2521 catataaata ggtctggtca cgcttgctgc ttcgataaat cacacaaaac atacttttac
     2581 tctttaagat gccaggtttt ggcataagct aacatcaatg aaacccgcgc cacaagccag
     2641 ccagctgtag atttctgcgg tttgttgagt ccatgcccct ttataagtaa tccccccggc
     2701 aaaaaatgcc gcgccgggat tttaccgaac gcagaaaagc cactggaatt ctcccgtaag
     2761 ggattgttat attataacag ttcatcgtac tcattctgaa caggagacta ccaatgaaaa
     2821 tcaagagcat cagtaaggct gtattgctgt ttgcactgct aacctccacg agctttgctg
     2881 cgggtaagaa tgtcaatgtt gagttcagaa aaggccatag cagcgctcag tactccggcg
     2941 aaataaaggg atacgattac gatacatata ccttctatgc caaaaaaggt cagaaagtac
     3001 atgtgagtat ctctaatgaa ggcgctgata cttacctttt cgggccagga atcgatgatt
     3061 ctgttgattt atccagatat tccccagaac tcgatagtca cggacaatac tcactccctg
     3121 cttctggcaa atatgagctg cgagtgctgc aaacccgaaa cgacgcccgt aaaaacaaaa
     3181 ctaaaaaata taacgtcgat attcaaatta agtaaatgtc aaccgacggg gactctcccc
     3241 gtcataaacg ttaacgcccc actcctctct gtcgctcaag aggttaatga cgaatagcag
     3301 acaatgccga atagaacata atgtaaacaa ggcatcactc acgacacata aataacatct
     3361 ttgtggtgat ttgattgatt tttccactcc gcttaccatc cacaccaaaa taacgatcat
     3421 cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg
     3481 gatcgtagcc ttgtaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg
     3541 ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc
     3601 acgccggaga ttgaagtccg ccagcatcat gtccagacgc cattgatgag aacatcatcc
     3661 ccacacctga caatccgata taaaaaatac gcatcatatt catagcacat cctgataatc
     3721 caaccgtaca acacttatgc tcatttcatc atcaatccgt aatcaaaacc gctgacctgg
     3781 acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta tgatagagca
     3841 attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg caaaaaatgg
     3901 aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg gatatttaaa
     3961 cagttctgga gatgctgtca cgattaggat gcggaaaaag gaagcgtaaa taagaagaat
     4021 caaaagtacg gcggcaatgg gttgattgac aagggtattt tttaagctat gaatcagcgc
     4081 catttatcac agaatagact tttactctga ataaatggga gggtgacttg cctcaatata
     4141 atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa
     4201 tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag gcagtagctt
     4261 cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc aggcgctgga
     4321 agacaaaatc gctcaggccc ccgcattttt aaaacatgcc cccgttgtac tcaacgtcag
     4381 tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg caaccggttt
     4441 gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaagat
     4501 ggggctgcct atcctgacgg aaggaaagga aaaagcgccg cgtccagctc ccgcaccaca
     4561 ggctccagcg caaaatacaa cgccggtcac aaaaacgcgt ttaatagata ccccggtgcg
     4621 ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc acgttagcgc
     4681 tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc
     4741 gcttgcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaatc tgatggcgga
     4801 actggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag aattttatgg
     4861 caaagcggcg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc
     4921 cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg caaagggggt
     4981 gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa gggaaagaaa
     5041 actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat gggttgtgaa
     5101 cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg
     5161 ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca aacacgcgat
     5221 aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa agcgatggat
     5281 tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt aatggcactc
     5341 tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt acgcgactct
     5401 gaccgtattt taggcattct ggcgtcgaaa tcacgccgcg cagaaaatgg cgaagagcct
     5461 attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag aggtgacatg
     5521 ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag
     5581 gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga cattaacgcc
     5641 gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga acgtcctttc
     5701 cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg ataagttatg
     5761 gcattactcg atttctttct ctcgcggaag aaaaacacag ccaacattgc aaaagaacgg
     5821 ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta tctgccgcag
     5881 ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc tgagatggta
     5941 accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa cgtgacctta
     6001 ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt caaggcagag
     6061 tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc ggcgagtatt
     6121 cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag gctgttccca
     6181 cgcacagaac aaaatgttta tgcctggttg agtaaataac ctcattgtta gtgtggatac
     6241 actgggggat gataaccccg caatttcatt attcattgtt ctcccatagg atgaggtttc
     6301 ccgccgtaac tggcgggctt tttttgcccc agaattttcc cttttagccc cctgtaaacg
     6361 aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt cacaccagcc
     6421 accgcattcc acggggattc cacaccggca ccatggctat accccacccc gagatgccgc
     6481 ttaacgtact gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca
     6541 aatcattgac gaaattaccg tcattattca ccttcacccg gttatcatcg acaaattctt
     6601 tgcgcacagc cgccttcagc cacggctcaa tttccatacc gttccccaga cgcatgttgt
     6661 aactcagcgt tgcacccagt tcacgatata tactgcgggt atcgactgat ttcgattcca
     6721 tgccattgga taaatgatag tagcgtctaa gtgacccaag ctctgctgca acatcagacc
     6781 gcaggcacca gaccagggga ttcatcctga agagacagcg caggtgtatt gtgttcaccg
     6841 ctcaccaaag acatcatgat gaaatgatga tatcccgcat aagaaagagg cattttttaa
     6901 acgcagtgcg ctgaagtgta gttggataaa aaatcactct tttcagataa tactgtcgta
     6961 cgcttatctt tcgacaatga ggccgtcggc aaaataaaat gactaatgta atcgtt
//
LOCUS       DAZFAH010000211         7011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFAH010000211 DAZFAH010000000
VERSION     DAZFAH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.213"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..1403
                     /gene="adeQ"
                     /locus_tag="ACHJWB_004154"
                     /gene_synonym="yicO"
     CDS             69..1403
                     /gene="adeQ"
                     /locus_tag="ACHJWB_004154"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO4315687.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSISPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1456..1908
                     /gene="yicN"
                     /locus_tag="ACHJWB_004155"
     CDS             1456..1908
                     /gene="yicN"
                     /locus_tag="ACHJWB_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4315688.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2119..3309
                     /gene="nepI"
                     /locus_tag="ACHJWB_004156"
     CDS             2119..3309
                     /gene="nepI"
                     /locus_tag="ACHJWB_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4315689.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3350..3643)
                     /gene="yicS"
                     /locus_tag="ACHJWB_004157"
     CDS             complement(3350..3643)
                     /gene="yicS"
                     /locus_tag="ACHJWB_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4315690.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3865..4683
                     /gene="nlpA"
                     /locus_tag="ACHJWB_004158"
     CDS             3865..4683
                     /gene="nlpA"
                     /locus_tag="ACHJWB_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4315691.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4687..5610)
                     /gene="yicL"
                     /locus_tag="ACHJWB_004159"
     CDS             complement(4687..5610)
                     /gene="yicL"
                     /locus_tag="ACHJWB_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4315692.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5721..6905)
                     /gene="setC"
                     /locus_tag="ACHJWB_004160"
     CDS             complement(5721..6905)
                     /gene="setC"
                     /locus_tag="ACHJWB_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO4315693.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     IAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1750 a         1588 c         1774 g         1899 t
ORIGIN      
        1 tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct cctccaatgg
       61 acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg acgctttcgc
      121 gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt gcggggatga
      181 ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc ggcgcggcac
      241 aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc ggcagtattg
      301 cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg ctgaacgcct
      361 tctttgcctt cgtggtcgtg ggggcgatgg gcatctcctg gcagaccggg atgggcgcaa
      421 tattctgggg cgcagttgga ctatttttgc tcacgctgtt tcgtatccgg tactggatga
      481 tctccaacat tcccttaagt ttacgtattg gtatcaccag cggaattgga ttatttatcg
      541 ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacacg ctggtgatga
      601 ttggcgattt aagttctcac ggcgtgttgt taggtatttt agggtttttt attataaccg
      661 tgttgtcatc acgtcatttt catgccgcgg tgctggtttc tattgtggtg acgtcttgct
      721 gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccatttcg cctgatatta
      781 gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc gccggtatca
      841 ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt ggtgtaactg
      901 ataaagcggg cttaatagat agtaacggta aattccccaa tatgaataag gcgctgtatg
      961 ttgatagcgt cagttcggtg gcgggggcgt ttatcggcac ctcgtctgtt actgcctata
     1021 ttgaaagtac ttctggtgtg gcagtcggtg gtcgcacggg gctgactgcg gttgtggttg
     1081 gcgttatgtt cctgttagtt atgttcttct caccgctggt ggcgatggtt cctccttacg
     1141 caaccgccgg agcgttaatc tttgttggcg tgctgatgac ttcgagcctg gcgcgcgtta
     1201 actgggatga ttttaccgaa tcggtgcctg cgtttattac cacggtgatg atgcccttta
     1261 ctttctcgat caccgaaggg attgcactcg gctttatgtc gtactgcatc atgaaagtat
     1321 gcaccgggcg ctggcgcgat ctgaacctgt gtgtggtggt ggtcgcagct ctgtttgcac
     1381 tgaagattat tctggtggat tagtaactgg cgagggtttc cagatatcat gagttctgat
     1441 tacgcaggag aactcatgat ctggataatg ctcgccacgc tggcggtagt gtttgtggtt
     1501 ggttttcggg tgctgacatc cggggccaga aaagcgattc gccgtctcag cgatcggctg
     1561 aatatcgatg tcgtacccgt ggagtcgatg gtcgatcaaa tgggaaagtc agccggtgac
     1621 gaatttttac gttatttgca tcgcccggat gagtcgcacc tgcaaaacgc cgcgcaggtg
     1681 ttgctaatct ggcaaattgt cattgtcgat ggtagcgaac agaacctgct gcaatggcat
     1741 cggattttac aaaaagcccg tcttgccgcg ccgattaccg atgctcaggt caggctggcg
     1801 ctgggttttc tgcgcgaaac cgaacctgaa atgcaggata ttaatgcttt tcagatgcgc
     1861 tataacgcgt tctttcagcc tgccgagggc gttcactggt tgcattgaaa ccgtttcgtc
     1921 ttatcagcct acagaccgcg cctgacctgt cgggtggata agacgttcac gccgcatccg
     1981 gtaatctttg ccctgttccg tttcgatctt aactccagct caagcaacac cgtaaaagtt
     2041 gcaaaaacgt taaatgcgtc acacatttca atgttaaagt ggtcggcttt tcccctgaaa
     2101 catgccacgg gtaacaccat gagtgaattt attgccgaaa accgcggcgc ggatgccatc
     2161 acccgaccga actggtcagc cgttttctcg gtggcgtttt gtgtcgcctg tctgattatc
     2221 gttgagtttt tgcccgtcag tttgttgacg ccaatggccc aggatttagg catttcggaa
     2281 ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt
     2341 attacccaga caattcaggc tactgaccgc cgctacgttg ttattttgtt tgccgttttg
     2401 ctgacgctct cctgcttgct ggtttccttt gctaactcat tcagtttgct tttaatcggt
     2461 cgtgcctgtc tggggctggc gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg
     2521 cgtctggtgc cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct
     2581 attgcgctgg tgattgccgc gccgttgggc agttttttag gcgagcttat cggttggcgc
     2641 aatgtcttta atgcggcggc ggtgatgggc gtgctgtgta ttttctggat tatcaaatca
     2701 ttgccttcac tgccaggcga accctcgcat cagaaacaaa atactttccg cttattacaa
     2761 cgtccgggtg tgatggcagg gatgatcgcc atcttcatgt ctttcgccgg gcagtttgct
     2821 ttcttcacat atattcgccc ggtgtatatg aacctggcgg gattcagcgt ggatggctta
     2881 acgctggtgc tgttgagttt tggcattgcc agctttatcg gtacgtcgct ttcgtcattc
     2941 attcttaaac gttcggtaaa actggcctta gcaggcgcgc cgttaatact ggctgtgagt
     3001 gcgttggtgc tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt ggcgattatc
     3061 tgggggctaa cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac ccgctcgctg
     3121 gccgatcagg cagaaaaagc cgggtctatt caggtggcgg ttattcagct tgctaatacc
     3181 tgtggcgcgg caatcggcgg ttatgcgctg gataatattg gtctgacttc gccgttgatg
     3241 ttgtccggca cattgatgtt gctgactgca ttgttggtta ctgcaaaggt gaaaatgaag
     3301 aaatcctgat atcaggtaaa gaagcgttgg tcagtagttg ttgctgttct tacatatccg
     3361 ggcattctac cttacccaac gcttcccaat aattgctctg attattacgt tccatcgcca
     3421 cgatggcctc gcgtatatgt tgtttattgt tttcatctga caacattaac ttttcccacg
     3481 cttcatctga taatgacgcc tgtggcgtgc aaattttacg taaatcttgt aataccgtgg
     3541 ttttctcttt tgcggactgc agtgagctaa aaggcgactc ggcgtatacg atgcctggca
     3601 tagcgaaaaa agtgaaaata agcagtagtg tcgttggttt catggattca accttttaca
     3661 aatgtgtatc gaatgaagca taagtgtagt ttgctttcat cgctctcatc atgctgtacg
     3721 aaacgcgctt gcgtggtcag taagaagtgc cagactttat attccacttt tattcctttt
     3781 tattcttatc gatagcgttt cgttttttaa accgcagcga ccttaccgct atagtcaggt
     3841 aatcattaat aaaaggataa aaaaatgaaa ctgacaacac atcatctacg gacaggggcc
     3901 gcattattgc tggccggaat tctgctggca ggttgcgacc agagtagcag cgatgcaaaa
     3961 cacattaaag ttggcgttat aaatggcgca gaacaagatg tcgcggaagt cgctaaaaag
     4021 gtggcaaaag agaaatatgg cctcgatgtt gaactggtag gatttagcgg ttcgttgcta
     4081 ccgaacgatg cgacaaatca tggcgaactt gatgcgaatg tcttccagca tcgacccttt
     4141 cttgaacaag ataatcaggc gcatggttat aaactggtag cggtgggaaa tacgtttgtc
     4201 ttccctatgg cgggttattc caaaaaaatc aaaaccgtgg cgcaaataaa agaaggcgcg
     4261 acagtggcga tccccaacga cccgaccaac cttggtcgcg cacttttact gttgcaaaaa
     4321 gagaagctaa ttactctgaa agagggaaaa gggttattac ctaccgcgct ggatataacc
     4381 gataatcctc gtcatttgca gattatggaa ctcgaggggg cgcagctccc gcgcgtactg
     4441 gatgatccca aagttgatgt agcgattatc agcaccactt acattcagca gacagggctt
     4501 tctccggtgc acgacagcgt atttattgaa gataagaatt cgccgtatgt gaatattttg
     4561 gtggcacggg aagataataa gaatgcagaa aacgtgaagg aatttctgca atcttatcaa
     4621 tcacccgaag tcgctaaagc ggcagaaacc atttttaacg gtggcgcggt gcctggctgg
     4681 taattctcac tcatgccgcg ccggacgatt tatttttctg gcgcggcgac gggaatccat
     4741 tgaaatcaaa atcaccgatg acagaatcag cagcgttccc agccagtccg gtaatgtgaa
     4801 cgtgatcccc agcaacagca aagagagtag cgcgctactt aatggttctg cacagctcaa
     4861 aatgctggct tttggaccgc caattaattg tgctcctttc aggtacagac taaatgtcag
     4921 ggacgtacca atgaccacca aataaaaaaa cgccagaatc aaactgccgt taacgacaaa
     4981 gtttgttcct tgtctggcat aaaaaggcaa cagaatcaga ccgccaatca gcatactcca
     5041 gccgacgact ggtaacgtgc catagcgggc aattagcgtt gagggatagg tggtatagaa
     5101 tgcagcagca aatgccgagg caatgcccca gaacaacgcg gcaggagaga gcgataatga
     5161 cgtcggatta ccgtgtgtca ccaataaaaa agtcccgacc agcgatgtca aaatagcgca
     5221 gaaaaccaga atgcccgggc gcgatttacg caccagtgag aaccaggcga cgataatcgt
     5281 cggtgagagg aattgcagca ccgttgccgt ggctgcgttc gatttttcga tggttagcaa
     5341 aaaagtgagc tgtacagtta gcgcgccaac cacggaaaaa atcagcaggc taatggcatc
     5401 tttatgattg ttaataatag aaaagatttt atcgccatga acaaatgaca gcgtcagtag
     5461 aatcaaaccg gcgaatatca aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg
     5521 ctccatgatg tattgcgcgc agacccctga acttccccac aacacggcgg caatcagaac
     5581 gttcagcatc ccctttctgg tggaacccat ttttccctct gcatactgtt ttgtcgttta
     5641 tgtatgcagg catgatagca aaaatggcga ggatggctct gtttgcgcca cgccgatttt
     5701 tcttagccca gactggtgag ctaaatatct ttaataaaca gcaggcaaat catcgcaata
     5761 cccaacatcc ctatcgccag ccagaacaac gcatggtagc tccagatttc aaccataatt
     5821 ccgtcaacag agccggcgat aatccagccg acgcgtgaag tatttgcata taacgttgtc
     5881 gcagagccta ttttttcagg catcaagtcc tgaaaataaa gcatgccgat accacagaga
     5941 ataccaagga agatggcatt aagaatttgc aattccagct caaccgccgg agtcgtcgcc
     6001 atgagtacgc tggcgtaaaa acacattcca ctcacgatag caatgagcat taatagtcgc
     6061 ttgccaatac gtttcatgta atagcctgcg attaacatca tcggaatttc cagaccggca
     6121 gcgataccaa tcatttctcc agccagttta tcggttagat gcagttcatc aataataaat
     6181 agcggcatat ttatcatgta gagattattc gccgcccaca tcattgaaca gaccacaaaa
     6241 agtagccgcg tatcccgctt cctgtgggtg gagggtaaaa tttctacggg ttgggtaacg
     6301 acaggaatat ttctttgtat tgatggcaaa aacaaccaga ctatcagtcc gcaaacaaca
     6361 aatgcgatgg cagcggtgag atacatcact ttaaaactaa atcccattgc cagttcataa
     6421 gcgatcggtg gcccgataac ccaggcaagc gagatctgag cacgtaaaaa tgtactgaac
     6481 atgaccgtct cacggcctgt tctgtcggcg tgttcacggg cgagggcgaa catttgcggg
     6541 tttgcggtgg aagcaaaact actcagaagt acgcccgttg agaggagaat gaagtagttg
     6601 cgattccacg caaaaagcgt gcaggccagc actccaaata agcaacatag cagaatcagt
     6661 aatttacggt cgccttgttt atcggagtgc cttgccagaa attgactgac cagaattccc
     6721 ataatagcgc taccggtgaa gaaaaaacct accattatag gacgggcttt cagttcatct
     6781 gcgaggaata tacttagggt aggagtctga agagcgcccg caatacccgt cagaaaggcg
     6841 acaagtaaaa atgccgcggc agtgagatca agtatttttg atggagtggt agccgttttt
     6901 tgcattcttc tgaatcaccg ggcgaaaaaa tataactgag ttacaaataa gtaccagtcg
     6961 aatgagtatt gtattaagaa tttttgagta tcgctgatac aaccagaaag c
//
LOCUS       DAZFAH010000212         7000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFAH010000212 DAZFAH010000000
VERSION     DAZFAH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.214"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..814
                     /gene="gadX"
                     /locus_tag="ACHJWB_004161"
     CDS             <1..814
                     /gene="gadX"
                     /locus_tag="ACHJWB_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4315694.1"
                     /translation="HGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCV
                     ENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNL
                     RYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARI
                     ASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVS
                     YFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1182..1910
                     /gene="gadW"
                     /locus_tag="ACHJWB_004162"
     CDS             1182..1910
                     /gene="gadW"
                     /locus_tag="ACHJWB_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4315695.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2273..5386)
                     /gene="mdtF"
                     /locus_tag="ACHJWB_004163"
     CDS             complement(2273..5386)
                     /gene="mdtF"
                     /locus_tag="ACHJWB_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4315696.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMALLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5411..6520)
                     /gene="mdtE"
                     /locus_tag="ACHJWB_004164"
     CDS             complement(5411..6520)
                     /gene="mdtE"
                     /locus_tag="ACHJWB_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4315697.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6628..6906
                     /locus_tag="ACHJWB_004165"
     CDS             6628..6906
                     /locus_tag="ACHJWB_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315698.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKIVLASPLVITGGNEISICIPRHASHQFQLFMS"
     gene            complement(6907..>7000)
                     /locus_tag="ACHJWB_004166"
     CDS             complement(6907..>7000)
                     /locus_tag="ACHJWB_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO4315699.1"
                     /translation="TLKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1774 a         1683 c         1730 g         1813 t
ORIGIN      
        1 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
       61 tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcaagt
      121 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
      181 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt
      241 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      301 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      361 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
      421 aacaagagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
      481 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
      541 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
      601 acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
      661 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
      721 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg
      781 cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc
      841 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
      901 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
      961 aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg
     1021 ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt
     1081 cctaccaaat ctggcagttt ttgcgctaag aaacagtctg gcaacatttc attagtatac
     1141 tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg
     1201 tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata tcgctgcata
     1261 acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg
     1321 atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc
     1381 agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac cagtcacccc
     1441 ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga
     1501 tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga
     1561 tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct
     1621 ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga
     1681 gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgctt
     1741 ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg catactattg
     1801 cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg
     1861 gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga catattttgc
     1921 atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc
     1981 tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga
     2041 ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt
     2101 gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc tactttcagt
     2161 gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag
     2221 caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt
     2281 ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg
     2341 ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc
     2401 cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg
     2461 tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct atcggcgttt
     2521 tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg
     2581 aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt aagccgcgca
     2641 gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg
     2701 gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc agtgaaatgg
     2761 catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg
     2821 agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc atagcgtcac
     2881 cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc
     2941 gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca
     3001 tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg
     3061 gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt
     3121 agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccattg
     3181 cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg
     3241 ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg
     3301 cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg
     3361 acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga
     3421 tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga
     3481 cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc ccctggccgc
     3541 tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct tttgtaagat
     3601 aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca ccggaaggta
     3661 actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag gtcggtgtac
     3721 gcaggaacag cagcgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg
     3781 tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg
     3841 tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg gctttcagaa
     3901 tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc agcatggagg
     3961 agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc ataaaggcca
     4021 tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt tggatctgcc
     4081 ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg acacgctcga
     4141 cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga
     4201 acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa
     4261 taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc aaaaacagat
     4321 acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact tcctgaatag
     4381 aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gcggggaaat
     4441 aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg gcgtttgctc
     4501 cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg
     4561 aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat
     4621 cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca
     4681 caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac
     4741 cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt accagattgt
     4801 atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct
     4861 gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt
     4921 ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagaa
     4981 tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca ggtaatgaag
     5041 gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgtcccag
     5101 tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca
     5161 tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg
     5221 tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg
     5281 gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg
     5341 caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa
     5401 gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc
     5461 gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct
     5521 gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta
     5581 gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg gcgttgtggg
     5641 tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg
     5701 tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca
     5761 cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg gtctggctgt
     5821 agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt
     5881 gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc gtgagatcga
     5941 cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga
     6001 gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg
     6061 cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt
     6121 ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct
     6181 tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt
     6241 tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat
     6301 acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac
     6361 cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc
     6421 tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc gtcatggcgg
     6481 cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg
     6541 gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg
     6601 ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg
     6661 ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt caacgacaaa
     6721 aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact
     6781 ggtggataca cataccaggg gaataaaatc gttttagcct ctcctttagt aatcaccggg
     6841 ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca gttgtttatg
     6901 tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt
     6961 cagtttgtac ataatgttga atttctgaat ctttaatgtt
//
LOCUS       DAZFAH010000213         6988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFAH010000213 DAZFAH010000000
VERSION     DAZFAH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.215"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..286)
                     /locus_tag="ACHJWB_004167"
     CDS             complement(<1..286)
                     /locus_tag="ACHJWB_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4315700.1"
                     /translation="MSREDPQLRIRLPVEVKEKIEISAKANKRSMNAEIVQRLDTSFL
                     KDIHEDDVISAYEAKIIANNARHEISNIIFKRTFNEINKKITLGHTSFYIN"
     gene            407..574
                     /locus_tag="ACHJWB_004168"
     CDS             407..574
                     /locus_tag="ACHJWB_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HFO4315701.1"
                     /translation="MQKAKEMCQRRVRFPDDVKLAIERNGEKECRKFNTEIIYQLRKV
                     YGLTGEKNSVA"
     gene            647..1591
                     /locus_tag="ACHJWB_004169"
     CDS             647..1591
                     /locus_tag="ACHJWB_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4315702.1"
                     /translation="MTSIATALSTINVPFHGAELYVVNHNGEPYTPMKPIVEGMGMDW
                     ASQFTKMKQRFKTCIVKITMQLPGDEQRREIICLALRKLAGWLQTISPNKVRPEIRDK
                     VIQYQEECDDVLYEYWTKGHVVNPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEK
                     QAKAMITMWSSLKSHFGCSYKEIDEDQFAEALSLVARVPLEGEHLPAGSASENDEVAV
                     KMLDALREAIKTQTKCYGYPLKPGYRSLIHSPSGVLGLTENSLLMNLLNQLQDDGHDV
                     SGAAAELTTMFCYIVGVSKCLRDIQTHAEYINDKAGFF"
     gene            complement(1665..1826)
                     /locus_tag="ACHJWB_004170"
     CDS             complement(1665..1826)
                     /locus_tag="ACHJWB_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HFO4315703.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(1918..2463)
                     /locus_tag="ACHJWB_004171"
     CDS             complement(1918..2463)
                     /locus_tag="ACHJWB_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HFO4315704.1"
                     /translation="MSLSASQNDKNQIVRYKGRVLHTQNFSALCASDLELKKVSDAFT
                     QYWKTGYHPSLGKDAAFARPTEMLKLNVRHTHVDNQDYIPEDSDKKHTGKKSSWDAWK
                     NIASVQVKCIPTSDCFLVYSVNHNRDALVMFFVDADAHNITEQEEFKEAAITISYQFF
                     EKTKTKPMPLEEDLFSDKWKE"
     gene            complement(2758..3450)
                     /locus_tag="ACHJWB_004172"
     CDS             complement(2758..3450)
                     /locus_tag="ACHJWB_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO4315705.1"
                     /translation="MNTKPSIFSFESSCQIRMFMIDGEPWFVTKDVCNALNIDVTQAR
                     KLDKKGWNKKGLYSIQTPGGIQELSIVSESGLYILILRCKEAMTEGTRAFRFLEWVTG
                     EVLPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVMQAMKIENKTYSAPLKPGYR
                     SLIHSPSGVLGLTENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKCLRDIQT
                     HAEYINDKAGFF"
     gene            3846..4574
                     /locus_tag="ACHJWB_004173"
     CDS             3846..4574
                     /locus_tag="ACHJWB_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315706.1"
                     /translation="MEVFWIVVGVVAVIIYVLHQNKTKISDRTVVNHNKTIKTEDGEI
                     TINRTQVIEHTSTQFQKTGGNAPNISAPPAYDSEVIQTYYKQQELAKERQLIQPKPFT
                     AELPPGVSTRPAYHGRFPGDDISSQSPKKAPQAVSESARIPSVSPSKEESANRVSSGR
                     KQCLRCRINLPYEKFRKSSKNPDGLTKWCARCLDGPKNTRHMKWCPICNVRRKRTSFY
                     PNNQNADGLMAWCKTCWDEHKAKR"
     gene            complement(4596..4853)
                     /locus_tag="ACHJWB_004174"
     CDS             complement(4596..4853)
                     /locus_tag="ACHJWB_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315707.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRSLVEGSHLMKLLRDLDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTT"
     gene            5048..>6988
                     /locus_tag="ACHJWB_004175"
     CDS             5048..>6988
                     /locus_tag="ACHJWB_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HFO4315708.1"
                     /translation="MTVSTEVDHNEYTGNGVTTTFPYTFRIFQKSDLVVQVIDLDENI
                     TVLALDTDYAVTGAGGYRGGNIILSKALANGYQISISRELPVTQETDLRNQGKFFAEV
                     HEDAFDKLTMLIQQCRSLFRLALCKPSSIANWYDALNNRIRNLKDPESPQDAVTLSYL
                     EGYIEGGDSTLRPDITRTGAVGNNIQQAINLSIAGQTVITMKDDEIVSSLLNEYGVEI
                     EQRRGRVFNTEGVQLTTYSDPRSRFVTGDQYLNYVISKMIKADGVDKSQKTYILWSGD
                     STIVGVNSELWPPATIADFYAKRYGIIDVKNTSLGHSGKTLYEWGNWYIDQEWSVFSA
                     ADLIVLRWGINDPYYGYTLDQVKDALDRGLSKLRNNKSVSQQSIIIMSPNSTSDTPNG
                     RDEKWYEQLTAVLRAKAKQYKCMFFDTYGMWQDSRGSAGNWLDDPYGDGRGIHPNSTF
                     SAQIHKLLFEYIYGPCAVINGSSNLFSNNGSSIQFINSSYPPNTFHMGTSWWRTNPDD
                     ASWPLDGVIQVDRFVDGIVVQKNYGYSSDNARVCLMRIGRISENTWSTWRGNQYDMSG
                     ILQNGWSVGEARSGTYQKSAEGVVTLSAVLTGGNTSVGTVIMVLPEGFRPKNDISYIP
                     SGTDSSVALINILSNGKVTIQSY"
BASE COUNT         1992 a         1433 c         1584 g         1979 t
ORIGIN      
        1 ggtttatata aaaacttgta tgtccaagtg ttattttctt attgatttca ttaaaagttc
       61 ttttgaaaat aatatttgat atttcatgcc gtgcattgtt tgcaataatt tttgcttcat
      121 aagcagaaat tacgtcatct tcatgaatat ctttcagaaa gctggtgtca agacgttgta
      181 ctatttcagc gttcattgag cgcttattgg cttttgcaga aatttctatt ttttctttta
      241 cttcaacggg aagtctgatc cttagttgtg gatcttctct gctcatgttc ttcaccaaat
      301 attgagttac ttgcaatgat tgaagtatgc ctcaccgtgt gcttgacatc aatgacgcac
      361 ggtgtgacaa ttgttttgcc ccacggtggg gctaaggaga tatgaaatgc aaaaagcaaa
      421 agagatgtgt caacgacgag ttcgttttcc ggacgatgtg aagttagcta ttgaaagaaa
      481 tggcgaaaaa gaatgccgaa agttcaatac agagattatc tatcagctga ggaaagtgta
      541 tggactcact ggtgagaaga atagcgtggc ataaaacagc gaagcccaac ggtggccaga
      601 ccgtcagggc ttcagtatcg taaaactacg tataggaatt aacgacatga cgagtatagc
      661 aacagcgcta tctactatca acgtgccatt ccacggcgca gaactttatg ttgtcaatca
      721 caacggagaa ccgtacaccc caatgaaacc tatcgttgaa ggcatgggta tggattgggc
      781 ttcacagttt acgaaaatga agcagcggtt taaaacctgc attgtgaaaa tcacaatgca
      841 gcttcctggg gatgaacagc gccgtgagat tatttgtttg gcacttcgca aacttgctgg
      901 ctggttgcaa accatcagcc ctaacaaagt ccgccctgaa atccgcgaca aggtaatcca
      961 gtatcaggaa gagtgtgacg atgttctcta cgagtactgg actaaaggcc atgtggttaa
     1021 cccacgcaaa gctaaaaagg cgttgccggg taaaataacc actgaacagc aggaagccat
     1081 taaacaactc gtcatgagtc gcggtcagtc tctgccaaaa gaaaaacagg ctaaggcgat
     1141 gatcaccatg tggtcgtcac tgaaatctca ttttggctgt tcgtacaaag aaattgatga
     1201 agatcaattc gccgaagcgt tgtctcttgt tgctcgggtt ccgcttgaag gggagcatct
     1261 ccctgctgga tctgcatcag agaatgatga agtggcagtc aagatgttag atgcgcttcg
     1321 tgaggcaatt aaaacacaga caaaatgtta tggttatccg ctgaagcccg gctaccgcag
     1381 tctgattcat tcgccgtctg gtgttctcgg cctgacggag aactcactgc tgatgaatct
     1441 gcttaaccag ttacaggacg acgggcacga tgtatcgggc gcggcggcgg agctgaccac
     1501 catgttctgc tacatcgtcg gtgtgagcaa gtgcctgcgt gatatccaga cccacgcgga
     1561 gtacatcaac gacaaggcag ggttcttctg acagaacggc ggcacaggga tgtgcaaaac
     1621 ggaactatcg tgacatgtca caggccgctt tcgcggcctt gtttttaacg aatgccaccg
     1681 ccgcccgggc gggaatccgc agaacgccca ccgcagcggg agccgtcagc ggcagtatcg
     1741 ctgtcgtgct gacaacgacc ggcaaaggcc tgagttgaag ctaccagaga caacaaaacg
     1801 aacagtgcag caaatgcttt tttcatttga aatttccatc tataagccac ctcaatgtgg
     1861 cgtcaatgag tgtagcactg acttttgttt cgtccacaaa aaagcccgca gtgcgggcta
     1921 ttccttccat ttatcagaaa aaagatcttc ttctaaaggc attggttttg tttttgtttt
     1981 ctcaaagaat tgataactga tagtgattgc tgcctcttta aactcttctt gctcagttat
     2041 gttgtgagca tctgcgtcaa caaaaaacat aaccagcgca tcacgattgt gatttaccga
     2101 ataaactaaa aagcaatcac ttgttggtat gcattttact tgtacagacg ctatattttt
     2161 ccatgcatcc caggatgatt ttttaccagt gtgtttttta tcactatctt ctggaatata
     2221 gtcttggtta tcgacatgag tatgcctgac atttagttta agcatttctg tcggacgagc
     2281 aaatgcggca tctttaccaa gagatggatg gtatcccgtt ttccaatact gggtaaatgc
     2341 atcagatact ttcttcagct caagatcaga tgcacaaagg gccgagaaat tttgcgtatg
     2401 caatactcga cccttatatc tgacaatttg atttttatca ttctgcgacg cagacaaact
     2461 cataattttc cttattgtta cgagaatcaa agaaagcgct ggatacgcga cttgcgtgat
     2521 cttttgtcat agtaactttt acgtactcta cgcttccatt gaaagaacgt cttgcggcag
     2581 cttgcgctct acgcatctgc aatttttcgt ttcgcatgac attacctcat atctcataag
     2641 ttcattacac ggattaataa aaatgaaacc aatccgttta cccttgaggt aatagtacgc
     2701 tattcacctg caatctgtac agaattattt aaaggcacat ccctgtgccg ccgcccgtca
     2761 gaagaaccct gctttgtcgt tgatatactc cgcgtgagtc tggatatcac gcaggcattt
     2821 gctcacacca acgatgtagc agaacatggt ggtcagctcc gccgccgcgc cagatacgtc
     2881 gtgcccgtct tcctgtaact ggttcagcag attcatcagc agtgagttct ccgtcaggcc
     2941 gagaacacca gacggagaat gaatcaggct gcggtatccg ggcttcagtg gggcgctgta
     3001 ggttttgttc tctatcttca ttgcctgcat tactgctgac gccgtggcgt tggctacctg
     3061 gtcggcaacc atctttatgc gttcttcctg cgggagcgag tttttaatgt aacttccagt
     3121 gcggcggatc tgaggaagaa cctcacctgt aacccattca agaaatctga atgctctcgt
     3181 tccctctgtc attgcctctt tgcaacgcag aataaggatg tagagacctg attctgaaac
     3241 gatggatagt tcttgtattc caccaggggt ctgtattgaa tacagcccct ttttgttcca
     3301 gcctttttta tcaagttttc tcgcttgtgt aacatcaata ttcaaagcat tgcacacatc
     3361 tttggtgaca aaccaaggtt ctccgtcaat catgaacata cggatctggc aggatgactc
     3421 aaaggaaaag atggaaggtt tggtattcat ggcgatcacc tttgtagtta ggttaatcac
     3481 caccgctgag accaatcaga tggtggtgaa ctgtgcagag ttggtcttac cggctacaaa
     3541 ggaacccggc gcacctttcg gtgcccccac acagcccacc atagaatggg tgcgctttac
     3601 acataaaaaa accgcttatg cggcatatgt gcctctgtag taatccggga gaccaatccc
     3661 ggcactggat tttgccagtg cccggttact atggcacagg atgagtgcga tgtaaattta
     3721 ccgtaaaggt aatgataagc gctgagaaat atcaaaatca accgtatttg gttgatggag
     3781 tttaatgctt gatcacctga aagcaagata ttacctttaa ggtaatgtta ttgtgaggaa
     3841 aagcaatgga agttttctgg atagttgttg gtgtggttgc ggtgattatt tatgtccttc
     3901 accagaacaa gactaagatc tctgatcgca cggtcgttaa tcataacaaa acgataaaga
     3961 ccgaagatgg ggagataacg attaatcgta cacaggtgat agaacacacc tctactcagt
     4021 ttcaaaaaac tggaggtaat gcgcctaata tttccgcacc tcctgcttat gatagtgagg
     4081 taatccagac atattataaa cagcaggagt tagcaaaaga gaggcaactg attcagccaa
     4141 agccatttac agcagagctt ccacctggag tgtcaacgcg tccggcatat catggaagat
     4201 tccctggtga tgacatatcg tctcagtcac ctaaaaaagc acctcaggca gtatcagagt
     4261 cagcaagaat accttctgta tcgccgtcaa aagaagaatc agctaacaga gtttcaagtg
     4321 gccgcaagca gtgtttgcga tgcagaataa accttccata tgaaaaattc cggaaatcgt
     4381 caaaaaatcc agatggattg actaagtggt gtgcaaggtg tctcgatggc ccaaagaata
     4441 cacgtcatat gaagtggtgc ccaatttgta atgtccgcag aaaacgaacc agcttttatc
     4501 ctaataatca aaatgcggat ggtttaatgg catggtgcaa aacgtgctgg gacgagcaca
     4561 aagcgaaacg ataggccgct cttgcggcct tttctttatg tagtttgttt tcgtaattgt
     4621 tcggcacaat agtcgagatg tgtttgcaga tcctgcatag acatctgtga gctggtgacg
     4681 tagttaatca gtgcagtcag ttcggcaagt gggccatcga cattaaatcc atccttatcg
     4741 agatcccgga gtaatttcat caagtgcgat ccctccacca gtgacctgac gcctcccggc
     4801 gtgtgaatcc tttcggtaaa tccgtcttcc agtggatagt gatactgctg catcttatct
     4861 tctccatgca atagctgtat aaatatacag tatcaaataa tttgtttgct atccagcacg
     4921 ttttgcgaat cacctgaaag gtaatatctg ttcgtattta tgggttatct atccatatat
     4981 ggttttaagg taatagaata accggatatg cggcgcaacg ggtgctgcga ctatctggag
     5041 atttaacatg acggtctcaa ccgaagttga ccacaacgaa tatacaggta acggtgttac
     5101 aactacgttc ccttatacct ttaggatttt ccagaaatct gatctggtgg tgcaggtgat
     5161 tgaccttgac gagaatatca ctgtgctggc tcttgatact gattacgcag tcactggtgc
     5221 gggaggctat agaggcggta atataatact gtcgaaggca ttggccaacg gttatcagat
     5281 ctctatatca cgagagctcc cggttactca ggaaacagat ctgcgtaatc agggtaaatt
     5341 ctttgcagag gtgcatgagg atgcgtttga taagctaacg atgctgattc aacaatgtcg
     5401 tagcttgttt cgtcttgcac tttgcaaacc atcaagtatt gcaaactggt acgacgcttt
     5461 aaataacagg attagaaatc ttaaagatcc agaaagccct caggatgcag taactttaag
     5521 ttatcttgaa gggtatatag aaggtggtga tagtactttg cgtccagata ttacaagaac
     5581 tggagctgta gggaataata tacagcaagc cataaattta tctatagccg gtcaaactgt
     5641 aattacgatg aaagatgatg aaattgtatc ttcattgtta aatgaatatg gtgttgaaat
     5701 tgagcaacgc agggggcgtg tatttaatac agaaggtgtt caactaacaa cgtattctga
     5761 ccctcgttcc cgttttgtaa ctggtgacca atatcttaat tatgttatta gcaagatgat
     5821 aaaagcagat ggcgtggata aatcacagaa aacatatatt ttgtggtctg gtgactccac
     5881 gattgttggt gttaactctg aattatggcc accagcaact attgctgatt tttatgcaaa
     5941 gaggtatgga attattgatg taaaaaatac atcgttaggt cattcaggta aaactctata
     6001 cgaatggggt aattggtata ttgatcagga atggtctgtt ttttctgctg ctgatttgat
     6061 tgttttacga tggggaatta acgatccata ttatggttat acattggatc aagtaaaaga
     6121 cgcattagat agaggccttt ccaagttacg aaataacaaa tcagtttccc agcaatcaat
     6181 tattatcatg tccccaaact caacatcaga cacaccaaac ggacgtgatg aaaaatggta
     6241 tgaacagtta acagccgtac ttagagccaa agcaaaacaa tataagtgta tgttttttga
     6301 tacttacggt atgtggcagg attcgcgagg atctgctggt aactggcttg atgaccctta
     6361 tggcgatggc aggggaatac acccaaactc aacatttagc gcacagatac ataaattatt
     6421 atttgaatat atttatggac cgtgtgctgt tataaatggg agtagcaacc ttttttctaa
     6481 caatggttct tccatacaat tcatcaatag ctcatatccg ccaaatactt ttcacatggg
     6541 gacttcatgg tggagaacaa accctgatga tgcttcttgg cctcttgatg gtgttattca
     6601 ggttgataga tttgttgacg gaatagtagt acaaaaaaat tatggttatt cttcagataa
     6661 tgctcgcgtt tgcttaatga ggattggaag gattagtgag aatacatgga gtacatggcg
     6721 aggtaatcag tatgatatga gtggcatatt gcaaaatggt tggtctgtag gtgaggcaag
     6781 aagtggaaca tatcagaaat cagccgaggg cgttgttact ttatctgctg ttttaacagg
     6841 tggaaataca tctgttggca cggttattat ggtattgcca gaagggttta gaccaaagaa
     6901 tgatatttct tatattccat caggtactga ttcatcggtt gccctcatta atatactttc
     6961 aaacggtaaa gttacaattc agagctat
//
LOCUS       DAZFAH010000214         6959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFAH010000214 DAZFAH010000000
VERSION     DAZFAH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.216"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            190..708
                     /locus_tag="ACHJWB_004176"
     CDS             190..708
                     /locus_tag="ACHJWB_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4315709.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            919..3060
                     /locus_tag="ACHJWB_004177"
     CDS             919..3060
                     /locus_tag="ACHJWB_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4315710.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGSIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3136..>6959
                     /locus_tag="ACHJWB_004178"
     CDS             3136..>6959
                     /locus_tag="ACHJWB_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO4315711.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPGETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVLKQHKGHPLA
                     RLWRALPEAVRLSPHTYMMAVSTTGQWLILGWPERVPEADEVPPELPAYRVLTGVVDG
                     FGRTLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLTTQAQRAEAFRKQRATSLSSPAGP
                     RSASSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDPGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLISRKDAQGRETRYEYSAAGDLTATISPDGKR
                     SATEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGEPAEQWQYDDHGWLTE
                     ISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLRSRHLNLPQLDREYTWNDNGQLVRISGPQESREYRYSDTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPE
                     GVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQM
                     EAEYIPERKLHLYHCDHRGLPQALISPEGETAWCGEYDEWGNLLGETSAQHLQQSLRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLKGGWNLYAYPLNPVQRGDPLGLSG
                     ETLVLGSATTVTG"
BASE COUNT         1600 a         1837 c         2278 g         1244 t
ORIGIN      
        1 ttattgctga ataagagatt taatgatttt aaaaatagtt tttacgacaa ccgtaaattc
       61 tagctcggtt tgtatgtaaa aaatactgaa aaatgatgcg gttccgtgaa gtgagctgca
      121 tctgcctgtc tgggattaac ggcaggtcat tgtaagtagt aattaaccac gttaacaagg
      181 agtttacaaa tgccaacacc gtgttatatc tccatcactg gtcagaccca gggaaatatt
      241 actgcaggtg catttaccgc tgattctgtc ggcaacatct atgtgcaggg gcacgaagac
      301 gagatgctgg tgcaggaatt cctgcataac gttactgtcc cgaccgatcc tcagtccggt
      361 cagcctgcgg gtcagcgtgc tcacaagcct ttcattttta ccgtggcgct gaacaaagcg
      421 gttccgctga tgtacaacgc actggcctcc ggcgaaatgc tgccaaccac tgaacttcac
      481 tggtggcgta cctctgtgga gggcaaacag gagcattatt tcactacccg cctgactgat
      541 tcgacgattg tcgatatgaa actgcatatg ccacattgcc aggatccggc gaaacgcgag
      601 ttcacccagt tgctcgaagt ttcactggcc taccgcaaaa ttgagtggga acacgtcaaa
      661 tcaggcacct ctggtgccga tgactggcgc gcaccgctgg aagcataagt taagccaaca
      721 gcatccgtct atcaggccgg atgctgtttt tgtattacga cgtaataaag aaaggttgtc
      781 agtaaggggt tacgttcacg gaagatggcg gatgccggaa acttcggcgg tagcgtacct
      841 gaacgcaagc gtaacactgt actgtcacag ccgctttaac agcgaactgt ctgtttttcc
      901 ttaaccccgg aggttgctat gtcaaccgga ttacgtttca cgctggaagt ggacggcctg
      961 ccaccggacg cgtttgcggt ggtctccttt catttgaacc agtcactctc ttcgctcttt
     1021 tccctcgctc tctctctggt cagccagcag tttctctccc ttgaatttca gcaaatactg
     1081 gacaaaatgg cctacctgac gatatggcag ggcgatgacg tacagcgccg ggtgaaaggt
     1141 gtggtgacct ggtttgaact gggggagaac gacaaaaacc agatgctgta cagcatgaag
     1201 gtgtgcccgc cgctgtggcg cacagggctg cgccagaact tccgtatctt ccagaatgag
     1261 gacatcgaaa gcatcctcgc gacgatcctg aaagaaaacg gtgtgaccga gtggagtccg
     1321 ctgttcagcg agccgcatcc ttcccgtgag ttttgtgtcc agtacggcga aaccgattac
     1381 gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa
     1441 aaaagtaccg accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt
     1501 gagatcccct ggaatccgaa cacccgtacc gaagtaagca ccctctgcat cagccagttt
     1561 cgctacagcg cacaaatccg cccttcttcc gtggtgacca aagactacac ctttaaacgc
     1621 cccggctggg ccggacgttt tgatcaggaa ggccagcacc aggattacca gcgcacacag
     1681 tatgaagtgt atgactaccc cggacgtttc aagggtgccc acgggcagaa ctttgcccgc
     1741 tggcagatgg atggctggcg aaacaacgca gaagtggcgc gcggaacaag ccgttctccg
     1801 gagatatggc cgggacggcg aattgcgctg acggggcatc cgcaggcgaa cctgaaccgg
     1861 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggacgc
     1921 agtggttcag gcaccacgct gaataatcac tttgcggtga tcccggcaga cagaacatgg
     1981 cgaccacagc cgttgctgaa accgctggtg gacggcccgc agagcgctgt cgtgacggga
     2041 ccggcaggcg aggaaatctt ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg
     2101 gaccgttata acccgtcaaa ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg
     2161 gcaggcaccg gattcggtaa cctggcgata ccgcgtgtgg gtcaggaggt gattgtggac
     2221 ttcctcaacg gcgatccgga ccagccgatc attatggggc gcacctacca ccaggaaaac
     2281 cgcacacccg gcagcctgcc gggaacaaag acgcagatga ccattcgttc gaaaacctat
     2341 aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga acaggtctac
     2401 atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac ggatgtgata
     2461 aacaaccatg ccgaaaaaat aggtaacaac caggcgatca ccgttaccaa taaccagatc
     2521 cagaacattg gcgttaatca gatacagacg gttggtgtca accaggtgga aacggtgggc
     2581 agtaaccaga ttatcaaagt gggatcaaac caggttgaaa aggtggggag cattcgtgcg
     2641 ctgacggtgg gtgtggctta ccagacgacg gtaggcggca ttatgaatac ctcggtggcg
     2701 ttgttgcagt cctcacaggt agggctgcat aaatcactga tggtgggaat gggctacagc
     2761 gtcaatgtgg ggaataacgt caccttctcg gtgggcaaga cgatgaagga aaacaccgga
     2821 caaacagcag tttattctgc cggtgagcat cttgaactct gctgtggtaa ggcaaggctg
     2881 gtgctgacga aggacggaag catatttctc aacggtacgc acattcatct ggaaggggag
     2941 tcggatgtga acggtgatgc gccagtgatt aactggaact gtggtgccac acaacctgta
     3001 ccggatgcgc ctgtgccgaa agatttaccc cccggaatgc cggatatgcg gcaattttga
     3061 gtcaggtgca aaaaattaaa acggaactgg tgcagtgacc agatgaacca tattaaaagc
     3121 agaaggaatt gattcatggg cggcggaaaa ccggcggcgc ggcagggcga catgacccgc
     3181 aagggactgg atattgtgca gggttcagca ggggtgctga taggcgcgcc aaccggtgtg
     3241 gcctgctcgg tgtgtcccac aaaaaaggac agtccgaact atggcagccc ggtgaacccg
     3301 ctgctgggag caaaggtgct gccgggcgag acggaccttg cgctgcccgg cccgctgccg
     3361 tttattcttt tccgtgccta cagcagctac cggaccagga cgcccgcgcc agtgggcgtg
     3421 tttggtcccg gctggaaagc gcccttcgat atccgcttac agatacgcga tgaaggcctg
     3481 atactcaacg acagcggtgg caggagcatt cactttgagc cgttgtttcc cggcgagata
     3541 agctacagcc gcagcgagtc gttctggctg gcgcggggcg gagtgctgaa acaacacaaa
     3601 ggccatccgc ttgcgcggct ctggcgggcg ttgccggaag cggtccgtct gagtccacac
     3661 acctatatga tggctgtcag cacaaccggg cagtggctga tactcggctg gccggagcgg
     3721 gtgccggagg cggacgaggt gccgccggag ctgcccgcat accgggtgct gacgggcgtg
     3781 gtggacggct tcgggcggac gctgactttc caccgggcgg cggagggtga cgtggcgggc
     3841 gcggtgacgg gggtgacgga cggcgcgggc cgctgttttc acctggtgct gaccacacag
     3901 gcgcagcggg cggaagcctt ccgtaaacag cgcgccacgt ctttatcttc ccctgccggt
     3961 ccccgttctg cttcctcttc ttctgctttc cccgacacgc tgcccgccgg tacagaatac
     4021 ggtgccgata acggtatccg gctggaggcg gtgtggctga cacacgaccc ggcatacccg
     4081 gatgaacagc cgactgcgcc gctggcgcgc tacacgtaca cggccagcgg agaactgcgg
     4141 gcggtgtatg accgcagcgg gatgcaggtg cgcgggttta cttatgatgc ggagcacgcc
     4201 gggcggatgg tggcgcacca ttatgcgggg aggccggaga gctgctaccg gtatgatgat
     4261 cccggtcggg tgacggagca ggtcaacccg gaggggctgg actaccgctt tgagtacgga
     4321 gagagccggg taatcatcac ggacagcctg aaccggcggg aggtgctgta cacggaaggc
     4381 gagggtggcc tgaagcgggt ggtgaagaag gagcatgccg acgggagcat cacccgcagc
     4441 gagtatgatg aggcggggag gctgaaggcg cagacggatg cggcggggcg gcggacggag
     4501 taccggctgc atatggcgtc gggcgcggtg acagcggtga cggggccgga cggcaggacg
     4561 gtgcggtatg gctataacag ccagcggcag gtgacgtcag tgacgtaccc ggacgggctt
     4621 cgcagcagcc gggagtatga tgagagggga aggctgacgg cggagacctc gcgcagcggt
     4681 gagacaacgc ggtacagcta tgatgacccg gcgagtgagc tgccgacagg gatacaggac
     4741 gcgacgggca gtacaaaaca gatggcatgg agccgttacg gtcaactgct ggcctttacg
     4801 gactgctcgg ggtacacgac gcggtatgag tacgaccggt acggtcaaca aatcgccgtt
     4861 caccgggaag aaggcatcag cacttacagc agttataacc cgcgtggcca actgatcagt
     4921 cggaaggatg cgcaggggcg tgaaacccgt tatgagtaca gcgctgcagg cgacctgaca
     4981 gcaacgatat caccggacgg taaacgcagc gccaccgaat atgataaacg cgggcgtccg
     5041 gtgagtgtga cggaaggtgg actgacgcgc agcatggggt atgacgccgc cgggcgcatc
     5101 accgtgctga ctaacgagaa cggcagccag tccacgttcc ggtatgaccc ggtggacagg
     5161 ctgacggagc agcgcggctt tgacggccgg acgcaacgtt accagtatga cctgaccgga
     5221 aaactcacgc agagtgaaga tgaggggctt atcaccctct ggcattacga tgcgtcggac
     5281 cgtatcacgc accgtacggt gaacggtgaa ccggcggagc agtggcagta tgacgaccac
     5341 ggctggctga cagaaatcag ccacctgagt gaaggccacc gtgtggcggt ccactacggc
     5401 tatgacgata aaggccgtct gacgggcgaa cgacagacgg tggagacccc ggagacgggg
     5461 gagatgctgt gggagcatga gacggggcac gcgtacagcg aacaggggct ggcgacccgt
     5521 caggagccgg acggtctgcc gccggtggag tggctgacat atggcagcgg ttatcttgcg
     5581 gggatgaagc tgggcggaac gccgctggtc gagtacacgc gggacaggct gcaccgtgag
     5641 acggcccgca gcttcggcgg ggcgggcagt acagcggggt acgaacaggc cacggcgtac
     5701 accctcacag ggcagctccg gagcaggcac ctgaacctcc cgcagcttga ccgtgagtat
     5761 acgtggaacg acaacgggca actggtgcgc atcagcggcc cgcaggagag ccgggagtac
     5821 cgttacagcg acacgggaag gctgacgggc gtccacacca cggcagcgaa cctggatatc
     5881 gatatcccgt atgcgacgga cccggcagga aaccggctgc cggacccgga actgcacccg
     5941 gacagcaccc tcacggcatg gccggataac cgcatcgcgg aagatgcgca ctatgtctac
     6001 cgccacgatg aatacggcag gctggcggag aagacggacc gcatcccgga aggggttatc
     6061 cggatgcacg acgagcggac ccaccactac cattacgaca gccagcaccg tctggtgttc
     6121 tacacgcgga tacagcacgg cgaaccacag gtggagagcc gctacctcta tgacccgctg
     6181 ggccgccgga cgggaaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg
     6241 ctgtcgcgta aaccggaggt gacatggtac gggtgggacg gtgacaggct gacgacgata
     6301 cagaccggca ccacgcgtat ccagacggtg taccagccgg ggagcttcac gccgctcctg
     6361 agaatcgaaa cagagaatgg cgaacaggcg aaggcgcggc accgtagcct ggcggaggtg
     6421 ttgcaggagg acacgggtgt gacgctaccg gcggagctgg cggtgatgct ggggaggctg
     6481 gagcgggaac tgcgcgcggg agcggtgagt gcggagagtg aggcatggct tgcgcagtgc
     6541 gggctgacgg cggaacagat ggccgcgcag atggaggcgg aatacatccc ggagaggaaa
     6601 cttcatcttt accactgtga ccaccgggga ctgccgcagg cgcttatcag cccggaaggg
     6661 gaaacggcgt ggtgcgggga gtatgacgaa tggggaaacc tgctgggcga aaccagtgcg
     6721 cagcaccttc aacagtcact ccgtctgccg gggcagcagt atgatgagga gtcggggctg
     6781 tactataacc ggaaccggta ctacgatccg ttgcagggac gctatatcac ccaggatccc
     6841 attgggctga aggggggatg gaacctgtat gcgtatccgc tgaacccggt gcagcggggg
     6901 gatcctcttg ggttgtctgg tgagacactg gttcttggtt ctgccacaac agtaacagg
//
LOCUS       DAZFAH010000215         6949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFAH010000215 DAZFAH010000000
VERSION     DAZFAH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.217"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..2784)
                     /gene="pqqL"
                     /locus_tag="ACHJWB_004179"
     CDS             complement(1..2784)
                     /gene="pqqL"
                     /locus_tag="ACHJWB_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4315712.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQRLRKNQ
                     DAFWEQMVNNEVAAKRALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGQEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEEKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2841..5213)
                     /gene="yddB"
                     /locus_tag="ACHJWB_004180"
     CDS             complement(2841..5213)
                     /gene="yddB"
                     /locus_tag="ACHJWB_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4315713.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYISESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5251..6936)
                     /gene="yddA"
                     /locus_tag="ACHJWB_004181"
     CDS             complement(5251..6936)
                     /gene="yddA"
                     /locus_tag="ACHJWB_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4315714.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMIIG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFIVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1797 a         1603 c         1563 g         1986 t
ORIGIN      
        1 ttattttggc aataatactc cggtataagt atttaccgga tgagaaagat attgtttaac
       61 ggcagtgtta acattctcta ccgtcatttg tttcaacaat tgctcctgct cagtccatgc
      121 tgcaggatcg tcatattgaa taagactatt tacaatagtg ttcgctaatt gttgaacgct
      181 acgctgttgg atatcgaggc tgcgctgaac gttttgctgg tattcattca gttcttgctc
      241 actgatccct ttagccagac gcttaaccat cacttcattc gctaacgtta acagttcatc
      301 atgtcgttct ggttgacaag taaaagccag caaatgactg atatctttgg cctgaggatc
      361 aaccgagagg cgagaagaaa cgctgtatgc cccagatgcc tgttcacgaa tattaacgcg
      421 tagatctttt gccagtgcga cgttaaaagc atcgagcgcc atacgcgtcg ccagattaac
      481 aggtgtccgg gaatcataac gcttccactg tgaaacctgt gccacaggtt cattttgttc
      541 ttttacagta accgatgcgt tgtccgtcgc gcgagttaat ggtttacctg cggctaatgg
      601 cgaatcagag tgtttgattg atcctaagta acgcgtaatt aacgccacga gtttttcttc
      661 tgcgacatta ccgacaatga caaacgtgat atccgctgga gatgaaaaca actgacgatc
      721 ggcagccagc gcatctgcgg cagtaaactg tgcaatctga ttttcttgca gtaatttcgt
      781 gcggtcatca gcatagcgcg tctcatacat ctgctgggcg aatttctccg ctggacgctg
      841 gtcgagcgtt ttcaacgcct gaatttgagc attttgtagc gatgcccaaa tattatcgtt
      901 aatcgtgctg tgggtgattc gctggttaat caactgaaaa ccaggttcag ggttattagt
      961 ccgcgcgcta acggagagca acgtattcat gccactgact ttactgctca tggttaccga
     1021 attttccgca ctccagcgtt tcaggctgga agaggagagt tcgccaacgc cgcttccgct
     1081 tactgcttta tttgccagcg cgataagtga tttttgctgc gcagggaaac ttaaatcgcc
     1141 tttattcgat acggcaataa tttgtagctt ttgctcttga cccgcggatt ttgccagaat
     1201 aaccctggca ccattggaaa gtgttaatga tgtcagattc tccgccagcg tttctttgct
     1261 gctaatttcc gcctgtgggt cagcgtctac tgttaacgat aaatttctgc cagggaagat
     1321 gtaagccgcc agctttttgt tggcgtactc tttttccagc gccaggatag ccgcaggaga
     1381 caatgctctt ttggcagcaa cctcattgtt taccatttgc tcccaaaatg cgtcctggtt
     1441 ctttcttaac cgctgccatt tttccgccag actttgcacg gtaatttgct gccacagacg
     1501 tttcgaaagt tgatatgtct cttccggcga caagaacggc gtattattta atgagctgga
     1561 tgccaggcga ctggtcagca tacgtaaatc acgttcagct tgctgatcaa ccgcattttt
     1621 cagccaggtg aggcgggtag atttgacatc atcgagttct tcagcagaaa aaccatgctg
     1681 atcaatggtt gccaactctg ccattaatgc attcgcagca tcctgcatat tatcgtctcg
     1741 tgcatttaca cggaaaaaca gcgactgata atcgggtgca attttaacgc tacgcgcagt
     1801 gccgccagaa atagtcttca actcgcccga ctgtatgcgt tcctgcagac gttgattgaa
     1861 cagctgaact aacatgctcc attcagcttg ttcgataaag ctttgctcat cgttcacttg
     1921 taccattggc aggcgataat agagtgcgat gccgttcacc cggttttctt tatcattaat
     1981 gatattaaag cgcaggtgat tttcggcttt tgtcggccag acgcgatttt cagctgcttt
     2041 gttagccgga agcttactta aattatcctt tatcagcgcc agcgcttctt tactgtcgat
     2101 atcgccgacc acgataaagg tcatattatt tggttgatac cagcgttgat aaaattgacg
     2161 caattgtgcc ggtgtgaccg tggcgacagt atccatcagg ccgataggtt cacggtctaa
     2221 attacgggta tttgccagca ggaaagggcg gcgcgcctga gaggtgcgcc atttcgcatc
     2281 ctgatgggca cgccattcct cagtaattac gccgcgttca gcgtctactt cgagtttttc
     2341 aaaggttgcg gcattactcc attcactgaa gattgccatc acttgttgca gattttgttt
     2401 ctgcgtagtc ggcagactca cctgatacac cgtttcgtca tagctggtat aggcattaac
     2461 atcgcgacca aaacgcaggc ccattgactc aaatgtttcg atgactttat tacccggcca
     2521 tgtttttgtg ccgttaaaca tcatatgttc tacaaaatga gccacgccgc gctcattgtc
     2581 ttcttcctgc aatgaaccgg tatgaatttg cagccataaa tttacctgat cctttggttg
     2641 agcatgcgga taaatcatat atcgcaagcc attgtccagt tgcccggtaa ttaacttttc
     2701 atcctgcggt aaggcggcgg caatcagccg cccggggagc aacagagttg ccactaacgt
     2761 cagtaagaaa cagaggtttc tcataattat ctccatgcga aaaccgggcg aatttacccg
     2821 gttaagtaaa atccgaacta ttaaaatttc atgctgacat caagccagaa agtacggcca
     2881 ctggcgtagg tcgccacacc ggtattcgtt gtatcaacag cggttttgct atccagtaca
     2941 ttgagaatat cggcactgat cgtcaggttt tgttgtttca gaaattgtgg cgtccaggac
     3001 aagcgggtgt cccaggtcag gctgctatcc agtttttcgt caacatactg cttgtaatct
     3061 gaatattcgc tgatgtattg cgcatttgtc ttacccagga taatgcgagc tttacgcgct
     3121 tcttgccagg ccagcgtatt cgcccacact aacccgctcg gttgatgcgt gaaatccatg
     3181 tttaaggaga tctttaatgg gttattaaaa tctgccactg gaacgctatc gtaagagacc
     3241 agattaccgt tataaaccac ctggttatca ccggtattgc tctcctcata accattgttt
     3301 aacgacaagt tgcccttgct cttgatatag ctaaagacaa tttgtgggtt aatatctacc
     3361 tggcggatat gcaggggttc ggccagctca aaactgaggt tgaacgaatg cgttttggtt
     3421 ttgccgtcgt tgttatattc agtaatggtg gttttagtcg cgctgtcggt acgactgctt
     3481 ttgctgattt gatcatgcgc ttcacggtaa acatagtttg cacgcgcaat aacgttctta
     3541 ccgattttct gctgcaatcc cattgccagt tcatcgttat aaggtgtttt caaatcctga
     3601 taacgcgtca gggttttatt acctgatacc gattccgtcc agctattgcg gatatcacgt
     3661 aatcccatat caagaatatt cccgccatag taacggttat aaccggcggt aatcattgag
     3721 gtttgatcag caaaaatatc ccattccgtc ataaagcgcg gggagatatt gtggtttgac
     3781 agatagttgt catagtcata acgcacaccg ggcattaacg acacattccg ccagctaatg
     3841 tgatccgcca tatacagcgt gtagttgtca attcccaggc tgcctttacc tttatggtaa
     3901 atggtatggt tagttttctt tcctgcagca ttaatcacat aggattcaga ctggttatgg
     3961 cgttcagtcc acgcatcgga atagatgtat tccgcgccga agtagggttg atgcgaaaca
     4021 ttacccacgg cgaatttttg ccagtccagg cgtgttttga aggtgtaatt atctacagcc
     4081 tgggaaatgt gtcctaatcc gccacgggtg caacgccctg taatatcacc atatgtacat
     4141 gaaagttcgg tgtaccagat gtcatgatcg tgacgggtat aatcactaat atgatcccaa
     4201 ccaacggtgg tacgaagttt ggcccatgcg agctgcgtat ccatatccca tgccagacca
     4261 taggatttat tacccatttc gcgatcagac tgcgggaagg tgctggtatt ataatcacgg
     4321 ctggagccgg tatattttaa ggttaaatcg tgggtaaagc ggtcgctggc aaaccaggta
     4381 aatttgctca atgcagtatc gataacgttt ttatactgtg cccgaccggc gacaatgccg
     4441 tcattcgaaa cataatccgc gcgggtgata tcagactggc ggcgcgataa accggcggta
     4501 acgccgaagt tatcagcgag ttcctgatta aacgacaagg tataaaagtt ctttttaaaa
     4561 tctggggagt aataagtact tcctgaagag ccttgattaa atgcgctctt gttattctca
     4621 tcgatatgcg atgttaacca gtccgaacgc gtagtgcgat aacccagttt cacgctgcta
     4681 tcatcagcgt tgaagcgttt gatctttgca tcaattaccc cgccattgaa gcgaccgaat
     4741 tcaaccggca caaaactgtc ataaagcgtc acattgtcga gtaagctgac atcaagataa
     4801 tacccctgtg acatcccgct aatattggtt gcactactag catcggactc attcgctggg
     4861 ttcaggttat tagtggcact aataccgtca atcaaatagg cattctgata gggcgacgca
     4921 ccgtgaatag agattttctc cgggcgaata tctccctggt tcaacgaggt actttgcgtt
     4981 gaatccatgc gtaccgcagg gttggttctc agcagatcgc tgatattgcc attcccggtc
     5041 ggtaaacgct cgatgctttc actgatgtaa tgcgtgttgc cgtttacggg tacagggaca
     5101 ggagcataga ccgtcatctc ttcaaaaaca tgcatgtaca tgtttttgtc atcgacggcc
     5161 tgcgccacat cagcgccaca taaaatgacg ccaggaataa gaactcgctt catttattta
     5221 tatctccgta cagcagggct tttattgttt ttataaaacc gcgctaatgt cacaaatatc
     5281 atcggccagg ttccagacac cgggttgatg tgtaaccata atgacgccgc ttgtgggtag
     5341 tttttcacgc actaaacgca gtaagcggat agcctcttgt tcctcaagat gagaggtagt
     5401 ttcgtcaaga aatatccatt tcggacgtcg taaaattaat cgtgccaggg cgatacgttg
     5461 tttttcgccg ctggaaagaa tatctcccca gcgatcgtgg tcatgaatac gcgcagccaa
     5521 tttcccaaga ccaacctgat gcagtacttc gctcaacgat ttatcgtcta cgggcagggg
     5581 aagtgcttta caaataatct ctttcagtaa gccggttttg attaacggtg tttgtgacac
     5641 ataccaactg tcagcaggag aagaaatatc acctttaaac cacggccagc agtgggataa
     5701 tgttttaagc agtgtggttt ttcccgcacc agagtagcct ttcagtaata gccatttgcc
     5761 tggcgaaaca tgaaagttca agttctctaa tatgatctta ttatcagggg tacgaatact
     5821 cgcgtcagcc acttgtaccg catgttggca atttttaggc ttattcgtag ggcgctgttc
     5881 ggtgagttga tggaactcat acaagcgatc gataaccgca gccagttcag caagttcatc
     5941 atatttataa ataaaccagc ttaaattgtt cgataccagc ataaatgcct ggcgcgattt
     6001 catcagtcca cccagattga tctgcccgct aataaactgc ggcaataaca gaaagtacgg
     6061 aagaacgcta agcgagcgcg aataaatatt ctgccagtaa tcaagccacc gctgacgatt
     6121 cattaaacga tgccagttct ctttaatggt atgaaaattg tcgctcaact cctggcgttg
     6181 tagagactca gcattgctta gcgcaatcag ttctgcctgc ttattatgct gcacaagatt
     6241 agttcgaaat gtcgcttcgc tacgttgttt ttccacatta agcggacgaa tacgcttacc
     6301 aaccttatgg gtaaataaag ttccaccgat cacaatgagc acaacggtat agaccatata
     6361 cccctggata ttccattctg ttccaccaac agtgaatgag agcgtacccg cgctttgcca
     6421 aagaataacg ataaaggtga tcagcatgct aagtgactgg atgaagccaa atgaaagact
     6481 gagcgttttg ctgatcagta agagaatgtc ttcagcgata cgttggtcag ggttatctgt
     6541 atttttatgc tcgccgtaga tctgcgtgaa gtagtagttt ttatctgcaa accagcgatt
     6601 aaggtagtaa tctgttaacc attcgcgcca gcgaatagtc agcagtttaa ttaaccatgt
     6661 tttgttcacg gatattaata caaagatccc cagcagggca gggaaccaaa ggacaagctg
     6721 ccaaagctta tcggtttctt tctggcttag cgcattgaaa aaatcattat tccagtcgtt
     6781 taaccagacc tgaattttaa ccacgccaat gatcatggcg aggataataa tgattaacaa
     6841 cactgaagtt ttattattct tacgcagcca aaagggcttc agcagacaga gatactttgc
     6901 tatcaacata caaaacgtaa tgggaatggt tatcattagc gaaaattga
//
LOCUS       DAZFAH010000216         6942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFAH010000216 DAZFAH010000000
VERSION     DAZFAH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.218"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..1801
                     /gene="pdeC"
                     /locus_tag="ACHJWB_004182"
     CDS             215..1801
                     /gene="pdeC"
                     /locus_tag="ACHJWB_004182"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4315715.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQEQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1804..2127)
                     /gene="soxS"
                     /locus_tag="ACHJWB_004183"
     CDS             complement(1804..2127)
                     /gene="soxS"
                     /locus_tag="ACHJWB_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4315716.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2213..2677
                     /gene="soxR"
                     /locus_tag="ACHJWB_004184"
     CDS             2213..2677
                     /gene="soxR"
                     /locus_tag="ACHJWB_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4315717.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3223..4572
                     /gene="ghxP"
                     /locus_tag="ACHJWB_004185"
                     /gene_synonym="yjcD"
     CDS             3223..4572
                     /gene="ghxP"
                     /locus_tag="ACHJWB_004185"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4315718.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4724..6373
                     /locus_tag="ACHJWB_004186"
     CDS             4724..6373
                     /locus_tag="ACHJWB_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4315719.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6527..>6942)
                     /locus_tag="ACHJWB_004187"
     CDS             complement(6527..>6942)
                     /locus_tag="ACHJWB_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4315720.1"
                     /translation="LNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSTMANVNFNNA
                     NLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQK
                     FFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1577 a         1609 c         1828 g         1928 t
ORIGIN      
        1 cattaagggc atatcttccc gccgcctctg cattcctgta ggaaattaat tttgaatatc
       61 aatgaattat tttcatccag gtgacgatta gaaaggtatc aatttcaaat caggcaaaag
      121 tgctatttat accgtaagat ttatctaaag acgtcggtac ccagggtttt caccttgcaa
      181 tggccgggta taaacaggca ggaaattgat agcaatgagt catcgtgcac gacaccaatt
      241 actggcgttg ccgggcatta tctttttagt tctctttccc atcattcttt cgctatggat
      301 tgccttcctt tgggcaaaat cagaagtgaa taatcagctc cgaacctttg ctcaactggc
      361 actggataaa tccgagctgg tcattcgcca ggcagattta gtgagcgatg cagctgaacg
      421 ctatcagggg caagtttgca ctccagccca tcaaaagcga atgttgaata ttattcgtgg
      481 ctatctttat attaatgaat tgatctatgc ccgtgataac cattttttat gctcatcgct
      541 gatagcgcct gtaaacggct atacgattgc accggccgat tataagcgtg aacctaacgt
      601 ttctatctat tattaccgcg atacgccttt tttctctggc tataaaatga cctatatgca
      661 gcggggaaat tatgtggcgg ttatcaaccc tctcttctgg agtgaagtga tgtctgatga
      721 tccgacattg caatggggtg tgtatgatac ggtgacgaaa acctttttct cgttaagcaa
      781 agaggcctcg gcagcaacgt tttctccgct gattcatttg aaggatttaa ccgtacaaag
      841 aaatggctat ttatatgcga cagtttattc gacaaaacgc ccaattgcag ccattgttgc
      901 gacttcatat caacgtctta taacccattt ttataatcat cttatttttg cgttgcccgc
      961 cggtattttg gggagtcttg ttctgctatt actctggcta cgtattcgac aaaactattt
     1021 gtctcccaaa cgcaaattgc aacgcgctct agaaaaacat caactttgtc tttattacca
     1081 gccaataatc gatatcaaaa cagaaaaatg tatcggcgct gaagcgttgt tacgttggcc
     1141 tggtgagcag gagcaagtaa tgaatccggc agagtttatt ccgctggcag aaaaggaggg
     1201 gatgatagaa caggtaactg attatgttat tgataatgtc ttccgcgatc tgggcgatta
     1261 cctggcaaca catgcagatc gctatgtttc tattaacctg tcggcctccg attttcatac
     1321 gtcacggttg atagcgcgaa tcaatcagaa aacagagcaa tacgcggtgc gtccgcagca
     1381 aattaaattt gaagtgactg aacatgcatt tcttgatgtt gacaaaatga cgccgattat
     1441 tctggctttc cgccaggcag gttacgaagt ggcaattgat gattttggta ttggctactc
     1501 taacttgcat aaccttaaat cattgaatgt cgatattttg aaaatcgaca aatcgtttgt
     1561 tgaaacgctg accacccaca aaaccagtca tttgattgcg gaacacatca tcgagctggc
     1621 gcacagcctg gggttaaaaa cgatcgctga aggcgtcgaa actgaggagc aggttaactg
     1681 gctgcgcaaa cgcggcgtgc gctattgcca gggatggttt tttgcgaagg cgatgccgcc
     1741 gcaggtgttt atgcaatgga tggagcaatt acccgcgcgg gagttaacgc gcgggcaata
     1801 aaactacagg cggtggcgat aatcgctggg agtgcgatca aactgccgac ggaaaacgcg
     1861 ggagaaggtc tgctgcgaga cataacccag gtccattgcg atatcaaaaa tcggacgctc
     1921 ggtggtgcgc aactcaacgg cggccagtaa caggcgacgt tggcgaatgt aatcgccaag
     1981 cgtctgatgc gtcaccgtgc ggaacattcg ttgcaagtac cactttgaat agcctgattt
     2041 ttttgcgact acatcaatgt taagcggctg gtcaatatgc tcgtcaatcc atgcgataag
     2101 atcctgaata attttctgat gggacataaa tctgcctctt ttcagtgttc agttcgttaa
     2161 ttcatctgtt ggggagtata attcctcaag ttaacttgag gtaaagcgat ttatggaaaa
     2221 gaaattaccc cgcattaaag cgctgctaac ccccggcgaa gtggcgaaac gcagcggtgt
     2281 ggcggtatcg gcgctgcatt tctatgaaag taaagggttg attaccagta tccgtaacag
     2341 cggcaatcag cggcgatata aacgtgatgt gttgcgatat gttgcaatta tcaaaattgc
     2401 tcagcgtatt ggcattccgc tggcgaccat tggtgaagcg tttggcgtgt tgcccgaagg
     2461 gcatacgtta agtgcgaaag agtggaaaca gctttcgtcc caatggcgag aagagttgga
     2521 tcggcgcatt cataccttag tggcgctgcg tgatgaactg gacggatgta ttggttgtgg
     2581 ctgcctttcg cgcagtgatt gcccgttgcg taacccgggc gaccgcttag gagaagaagg
     2641 taccggcgca cgcttgctgg aagatgaaca aaactaaagc gccacaaggg cgctttagtt
     2701 tgttttccgg tctttgtctt tctctctatc ccgctggtac acaggagggt ttcccccgac
     2761 gtcaacacac ctcattcgag cacgtggtgg aggttccggt tggtgttgat gctttaattg
     2821 tatgtcaccg acgtttcttc gccagtgtaa aagtatactt tttaaccgca atatttttgt
     2881 catctcagac gattttttat cgcaatcctg aacggtatac ggctcgataa cgctgcaatc
     2941 ttgcgcaccg acgataacgt ttgcgcatca attgcctgtt ttttcatcgt caagacaata
     3001 aaagagaaaa aagcagcaaa cttcggttga aaaagccgct atgatcgccg gataatcgtt
     3061 tgcttttttt accacccgtt ttgtatgcgc ggagctaaac gtttgctttt ttgcgacgca
     3121 gtaaattgtc gcaaacctgg agcaggaaga taacgtttcg ctggcagggg attgtccgcc
     3181 acgcatcttg acgaaaatta aactctcagg ggatgttttc ttatgtctac gccatcagcg
     3241 cgtaccggcg gttcactcga cgcctggttt aaaatttcac aacgtggaag cactgtccgt
     3301 caggaagtgg ttgccgggtt aacaacgttt ctggcgatgg tctactcggt catcgtcgtt
     3361 ccaggtatgt tgggtaaagc aggcttcccg cctgcggcag ttttcgttgc aacctgtctg
     3421 gttgccggac tcggttctat cgtgatgggt ctgtgggcta atctgccgtt ggcgattggt
     3481 tgcgccatct ccctgacggc atttaccgca ttcagcctgg tgctggggca acatattagc
     3541 gtacctgtcg cgctgggtgc cgtgttcctg atgggtgtgc tgtttacggt aatttctgcc
     3601 acgggtatcc gtagctggat tttgcgcaac ttgccgcacg gtgtggcgca cggcacgggg
     3661 atcggtatcg gtctgttctt gctgctcatt gccgctaatg gtgtcggtct ggtgattaaa
     3721 aacccgcttg atggtctgcc cgttgcgctg ggtgatttcg cgaccttccc ggtgattatg
     3781 tcactggtag gtctggcggt gatcatcggc ctggaaaaac tgaaagtccc tggtggcatt
     3841 ctgctgacca ttatcggtat ctcaattgtc ggtttgatct tcgatcctaa cgtccatttc
     3901 tccggcgttt tcgccatgcc ttcattgagc gatgaaaacg gcaattcact gattggcagc
     3961 ctggacatta tgggcgcgct gaatcctgta gtcctgccaa gcgttctggc gctggtgatg
     4021 acggcagtat ttgatgccac cggaactatc cgtgccgtcg ccggccaggc gaacctgctg
     4081 gataaagatg ggcagatcat cgacggtggg aaagcactga ccactgactc catgagcagc
     4141 gttttctctg gcctggtggg tgctgctccg gcagcggtat acatcgagtc tgcggcgggt
     4201 acggcggcag gcggtaaaac cggtttgacg gctatcaccg ttggcgtgct gttcctgctg
     4261 attctgttcc tctctccgct ctcttacctc gttccggggt atgcaacggc tccggcgctg
     4321 atgtacgttg gcctgctgat gctgagcaac gtggcgaaaa tcgactttgc tgattttgtt
     4381 gatgcgatgg cgggtctggt tacggcggta ttcatcgtgc tgacctgtaa catcgtaaca
     4441 ggcatcatga taggcttcgc gactctggtg attggtcgtc tggtttccgg cgaatggcgc
     4501 aagttgaaca tcggtacggt cgttatcgcc gtggcgctgg tgaccttcta tgcgggtggc
     4561 tgggctatct aatctttcat cggattctga aaacgggtgg caatggctgc ccgtttttat
     4621 tttctccgca cattgtgtga gcttttgcga tattctgaaa aaatgagaat tcaggcataa
     4681 cgcctttctc agatcataaa aaaacatcgc aaacagggaa cgcatggaaa tcttcttcac
     4741 catactgata atgaccctcg tggtctcgct gtccggggtg gtcactcgtg tcatgccctt
     4801 tcagatcccg cttccgctta tgcaaatcgc catcggtgcg ctactggcgt ggccgacgtt
     4861 tggtttgcat gtggagtttg atcctgaact ctttttagtc ttgtttatcc cgccgttgct
     4921 gttcgctgat ggctggaaaa cgccgacccg tgaatttctc gaacatggtc gagagatttt
     4981 cggcctcgcg ctggcgctgg tggtggtcac cgtggtcggc attggcttcc ttatttactg
     5041 ggtggtgccg ggcattccgc tgatccccgc ctttgcgctg gcggcggtac tttctccgac
     5101 cgatgctgtg gcgctctccg ggattgttgg cgaagggcgc atcccgaaaa aaatcatggg
     5161 cattttgcag ggcgaagcgt tgatgaatga cgcctccggc ctggtgtcgt tgaagtttgc
     5221 cgtggcagtg gcgatgggga cgatgatctt caccgtcggc ggtgcgacgg tcgaatttat
     5281 gaaagtagcc attggtggta ttcttgccgg ttttgtggtg agctggctgt atggtcgctc
     5341 gctgcgattc ctcagccgct ggggcggtga tgaacccgcg acgcagatcg ttctgctgtt
     5401 cttgctgcca ttcgcttctt atctgattgc cgaacatatt ggcgtttccg gcatcctcgc
     5461 tgcggttgcc gccgggatga ccatcacccg ctccggtgtg atgcgccgtg cgccgctggc
     5521 aatgcgcctg cgcgcaaaca gcacctgggc gatgctggaa tttgtcttta acggcatggt
     5581 gttcctgctg ttaggtctgc agctgccggg tattctggag acgtcgctga tggcggcaga
     5641 aatcgaccct aacgtcgaaa tctggatgct gtttaccgat attattctga tatatgcggc
     5701 gctgatgctg gtccgtttcg gctggctgtg gacgatgaaa aagttcagca accgcttcct
     5761 gaagaagaag ccgatggagt ttggttcgtg gaccacacga gaaatcctga tcgcgtcttt
     5821 cgccggggtg cgtggggcga tcactctggc cggtgtgctc tctatcccgc tgctcttgcc
     5881 ggatggtaac gtcttcccgg cgcgctatga gctggtgttc ctggcggctg gcgtcattct
     5941 cttctcgctg tttgtcggcg tggtgatgtt gcctattctg ctacaacaca ttgaagtcgc
     6001 ggaccattcg caacaattga aagaagaacg tattgcgcga gcggcaacgg cagaagtggc
     6061 gattgtggcg atccagaaaa tggaggagcg tctggcggcg gataccgaag agaatatcga
     6121 taaccagctg cttacggagg tcagttctcg cgtcattggt aacctgcgtc gtcgcgccga
     6181 tggacgtaac gacgttgaaa gttccgtgca ggaagagaac cttgagcgtc gcttccgtct
     6241 ggcggcattg cgttctgaac gtgctgaact gtaccacctg cgcgccacgc gggagatcag
     6301 caacgaaacg ctgcaaaaat tactgcacga tctcgatttg cttgaagcgt tgctaattga
     6361 ggaaaatcag taagccgaaa gcgacgtaac caggcccgag gtaatctgaa aaaggtgcaa
     6421 tcttttgcac ctttaacagg ttagctcggg ctaacattca tgattctaaa acaaataagt
     6481 tatctctctt gtaaagtcaa ttcattggct gttcttcgac ttaacctcaa tctgcgtgtt
     6541 gcataatatc tgcagtaatc ttcatagcga ctgcattaat attatcctca gtttgtgaca
     6601 tgccagtatg agaggtacaa tcttcttcaa agaatttctg tagtgttacc agggtatcac
     6661 tatcaatggg gatattttcc tccccacgga gaatggaaat gcccattccc tggacgctag
     6721 cctcatcaaa attaacccga tagagacggg tattaaacat acaggctttt gttaaaatag
     6781 aacagttgaa atggcagttg cttaaattgg cattattaaa attaacatta gccatagtag
     6841 agccggtaca tataaccatt tcgaggaatg tatttgtcag ccacgtacaa aagagtttgc
     6901 tatcaattaa gtttatattc tggaggattg ctttgttcag at
//
LOCUS       DAZFAH010000217         6940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFAH010000217 DAZFAH010000000
VERSION     DAZFAH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.219"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            118..729
                     /gene="ruvA"
                     /locus_tag="ACHJWB_004188"
     CDS             118..729
                     /gene="ruvA"
                     /locus_tag="ACHJWB_004188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4315721.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            738..1748
                     /gene="ruvB"
                     /locus_tag="ACHJWB_004189"
     CDS             738..1748
                     /gene="ruvB"
                     /locus_tag="ACHJWB_004189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4315722.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1895..2680)
                     /gene="znuB"
                     /locus_tag="ACHJWB_004190"
     CDS             complement(1895..2680)
                     /gene="znuB"
                     /locus_tag="ACHJWB_004190"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4315723.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIVIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2677..3432)
                     /gene="znuC"
                     /locus_tag="ACHJWB_004191"
     CDS             complement(2677..3432)
                     /gene="znuC"
                     /locus_tag="ACHJWB_004191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4315724.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3511..4443
                     /gene="znuA"
                     /locus_tag="ACHJWB_004192"
     CDS             3511..4443
                     /gene="znuA"
                     /locus_tag="ACHJWB_004192"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4315725.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4459..5781
                     /gene="mepM"
                     /locus_tag="ACHJWB_004193"
     CDS             4459..5781
                     /gene="mepM"
                     /locus_tag="ACHJWB_004193"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4315726.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5901..6872
                     /gene="lpxM"
                     /locus_tag="ACHJWB_004194"
                     /gene_synonym="msbB"
     CDS             5901..6872
                     /gene="lpxM"
                     /locus_tag="ACHJWB_004194"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4315727.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1804 a         1722 c         1806 g         1608 t
ORIGIN      
        1 tgccattttt cagatcatcg ggacacctcg caagttttct tcatccttcg ctggatatct
       61 atccagcatt tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg
      121 ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg
      181 ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg
      241 ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac
      301 ggttttaaca ataaacaaga acgcacgttg ttcaaagagt tgatcaaaac caacggcgtc
      361 ggcccgaagt tggccctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc
      421 gttgagcgtg aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc
      481 gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg
      541 ccagccgccg acctggtact cacgtctcct gccagcccgg cgaccgacga tgctgaacaa
      601 gaagcagttg ccgcgctggt ggcgctaggc tataaaccac aagaagcaag ccgcatggtg
      661 agcaaaatcg ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc
      721 gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc
      781 cggaagatgt ggcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc
      841 cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc
      901 tcgatcatct gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg
      961 tcgccaatga aatgggcgtt aatttgcgca cgacttctgg tccggtgctg gaaaaggcgg
     1021 gcgatctggc agcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga
     1081 tccaccgtct ttcaccagtg gtggaagagg tgttgtatcc ggcaatggaa gattaccaac
     1141 tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt
     1201 ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt
     1261 ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc
     1321 gcagcgcacg ctttatggga cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc
     1381 gcgctcgcgg tacgccacgc attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg
     1441 aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga
     1501 atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata
     1561 agttctttgg tgggccggta ggtctggata accttgcggc agccattggc gaagaacgtg
     1621 aaaccattga ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac
     1681 cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa
     1741 tgccgtaagt cggattggtc gctcgcgccg catccgacac tggcagtgtg tctgatgcga
     1801 cactgttcgc gtcttatcag gccagccaga gatagcgaac cgtaggtcgg ataaggcgct
     1861 cgcgccgcat ccgacaaatg tgttcagcga tagattagct ggcctgcttt ttcatcatac
     1921 tgagaataaa taacagtgcc gcacatagca ccaccgacgg acccgccggc gtatcgtaaa
     1981 acgcggaaaa ggttaaaccg ccagttactg ccaccatccc caccaaaaca gcgacaccag
     2041 ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc tgcaggaata atcagcagtg
     2101 aagtaataat caacgcgcca acaaatttca tcgctacacc aatcgtcaat gccgtcacca
     2161 gcatcaacaa caatttcacg cgctgtaatt tcacaccatc aacaaacgcc agatccgggc
     2221 taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa aatagccacc acgatgacca
     2281 cgccaatcac aatagcgatg agatcttctg gcgtcactgc cagcaaatca ccgaacaggt
     2341 aagccatcaa atcaacacga atgttagaca tcagactaac gaccaccagg cccagcgaca
     2401 gggcactgtg cgccataatc cctaataacg tgtcaatcgc cagctgtgga cgcttctcca
     2461 gccataccag accgcccgcc aggagcagcg taacggcaat taccgcatag aatggattca
     2521 cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc atgagccagc gtatcaccga
     2581 aataagacat acgacgccag actacaaacg aacccagcgg acccgcggca caggcgagca
     2641 tgatcccggc taaccaaccg ggaaataata attcaatcat gagcgatcat ttccccgacg
     2701 caaaacaatt cgtccctgta aatcgtgacg atgattatga tgatggcgat agatacccag
     2761 ttgttcagca ccacgaggac caaacataga aataaactcc ggatgtaggg aaacaacttc
     2821 cggtgtgccg gaacaacaaa tgtggtgatt cagacacagc acttcatcgg tttttgccat
     2881 taccagatgc agatcgtgag agaccattaa aacgccacaa tccagttcgc gacgcaactg
     2941 gtcaataagg tcatataacg ccacctggcc atttacatcc acgccttgag tgggttcatc
     3001 cagcactaat aattgcggtc gatttaacaa tgctcgcgct aacagtacac gctgtgtttc
     3061 gccaccagag agcttttgca tcggtgcgtt aatcagatgc ccggcctgga cacgtttcag
     3121 tgcaggcaaa atatcttctt tatgtgtgcc agggcgtaag cgtaaaaaac ggtttacggt
     3181 cagtggcaac gtggtgtcga gatacagctt ctgcggtaca tagccgatgc gtagttttcc
     3241 gttgcgcttg ataacccctt catcgggtgt taccagcccg agcactaccc gtaccagtgt
     3301 cgacttacct gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt taagttccag
     3361 cgacacatca gagaggacgc ggcgttggcc aaaagaaacc gagacatttt ccagggaaac
     3421 cagacttgtc atgttaattt tagtcttgca gtagtcatga aatgttataa tatcacactt
     3481 ctcatattca ttacgatgat tggtcgcatt atgttacata aaaaaacgct tcttttcgca
     3541 gcattatccg ccgctctctg gggaggtgca acacaggccg cagatgccgc cgttgtcgct
     3601 tcgcttaaac ccgttgggtt catcgcttct gccattgctg atggtgtaac agaaacggag
     3661 gttttactgc ctgacggcgc ttcagaacat gattattcac tgcgtccatc ggatgtaaaa
     3721 cgcttacaga acgcggactt agtcgtttgg gttggcccgg agatggaagc gtttatgcaa
     3781 aaaccggtaa gcaaattacc aggagcgaag caggtaacga ttgcgcagct tgaagatgtg
     3841 aaaccgctac tgatgaaaag tattcacggc gatgatgatg atcacgacca cgcggaaaaa
     3901 agtgacgaag atcaccatca cggcgatttc aacatgcatc tttggctttc cccagagata
     3961 gcgcgggcta cagcggttgc aatccatgga aaattagtgg aacttatgcc gcaaagtcga
     4021 gccaaacttg acgccaacct gaaggatttt gaggcacaat tagcctcaac cgaaacgcag
     4081 gttggtaacg agctcgcgcc gctcaagggg aaaggttatt tcgtttttca cgatgcttac
     4141 ggctatttcg aaaaacagtt cggactgaca ccgcttggtc attttaccgt taaccctgag
     4201 attcaacctg gcgcgcagcg tttacatgaa ataagaacac agttggttga gcaaaaagca
     4261 acctgcgttt ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg
     4321 acatccgttc gtatgggaac gttggatccc ctggggacga atatcaaact gggtaaaaca
     4381 agctattcag aattcctgag tcaattagcc aaccagtatg cgagctgcct gaaaggagat
     4441 taatgaggaa gtgattacgt gcaacagata gcccgctctg tcgccctggc gtttaataat
     4501 ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg ttcttactct ggccgtcgct
     4561 gtctggcggc cttatgttta tcaccgtgat gccacgccaa ttgtcaaaac cattgagctg
     4621 gaacagaacg aaattcgttc gctcttacct gaagccagtg agccgattga tcaagctgca
     4681 caagaagatg aagccattcc ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc
     4741 gtgcatgaat atgttgtttc cactggcgat acgctaagca gcattctcaa tcagtatggt
     4801 attgatatgg gtgatatcac ccaactggct gcggccgaca aagaattgcg taacctgaaa
     4861 atcggtcaac aactctcctg gacattaacc gcggacggcg aactgcagcg cctcacctgg
     4921 gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg
     4981 accagcgaaa tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac cgtcgggggg
     5041 agctttgttg ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt
     5101 aaagccatgc agtggcaaat ggatttccgc aaactgaaaa aaggcgatga atttgcggta
     5161 ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt
     5221 ttgcgttcag aaggtaaaga ttattacgct atccgcgctg aagatggcaa attctacgat
     5281 cgtaacggta ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa acagttccgt
     5341 atctcgtcta actttaaccc gcgtcgtact aatccggtga ccggtcgcgt tgcaccacac
     5401 agaggtgttg atttcgccat gccgcagggt acgccagtgc tttcagtggg tgacggtgaa
     5461 gtggtggttg ccaaacgcag tggcgcagca ggttattatg tggctattcg tcatggtcgc
     5521 agctacacca cgcgttatat gcacttgcgc aagattctgg tgaaaccggg acagaaggtg
     5581 aaacgtggcg accgtatcgc gctttccggt aataccggac gttcaaccgg gccgcatctg
     5641 cactatgaag tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt
     5701 accgaagggc tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg
     5761 ccgcagctac ggtttgatta attaacatcc attcgcagcc ggtacgcagt cagtaccggc
     5821 tttttttatt tggtgcgggg caagttgcgc cgctacacta tcaccagatt gatttttgcc
     5881 ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa tagcgaatac attcctgagt
     5941 ttgataaatc ctttcgccac ccgcgctact ggggagcatg gctgggcgta gcagcgatgg
     6001 cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat tctggcacgg ctgggacgtt
     6061 ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt aatcaatttg tcgctctgct
     6121 ttccagaacg tagtgaagct gaacgcgaag cgattgttga tgagatgttt gccaccgcgc
     6181 cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg accggagaaa attcagccgc
     6241 gcgttgactg gcaagggctg gagatcatcg aagagatgag gcgtaataac gagaaagtta
     6301 tctttctggt gccgcacggt tgggccgtcg atattcctgc catgctgatg gcctcgcaag
     6361 ggcagaaaat ggcagcgatg ttccataatc agggcaaccc ggtttttgat tatgtctgga
     6421 acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag aaatgacggt attaaaccat
     6481 tcatccagtc ggtacgtcag gggtactggg gatattattt acccgatcag gatcatggcc
     6541 cagagcacag cgaatttgtg gatttctttg ccacctataa agcgacgttg cccgcgattg
     6601 gtcgtttgat gaaagtgtgc cgtgcgcgcg ttgtaccgct gtttccgatt tatgatggca
     6661 agacgcatcg tctgacgatt caggtgcgcc caccgatgga tgatctgtta gaggcggatg
     6721 atcatacgat tgcgcggcgg atgaatgaag aagtcgagat ttttgttggt ccgcgaccag
     6781 aacaatacac ctggatacta aaattgctga aaactcgcaa accgggcgaa atccagccgt
     6841 ataagcgcaa agatctttat cccatcaaat aaaaaacagc ctctcaaatt attgagaggc
     6901 tgtacttgat aaaaaatctc aatgagtgtt ctcaccattt
//
LOCUS       DAZFAH010000218         6755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFAH010000218 DAZFAH010000000
VERSION     DAZFAH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.220"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHJWB_004195"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHJWB_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4315728.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHJWB_004196"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHJWB_004196"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4315729.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHJWB_004197"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHJWB_004197"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4315730.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHJWB_004198"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHJWB_004198"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4315731.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHJWB_004199"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHJWB_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4315732.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELCRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1474 a         1834 c         1827 g         1620 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatagtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaagcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc actgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggctg cgaaggtagc cgcaaagcca acagctgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacataacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac aggcccgcat tgtcgtaacc gcgggttttc agctccgcca
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaagt
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gctatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcatgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc gaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatgccggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggatta
     6121 tcgaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc tgtaatctga
     6661 cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tatta
//
LOCUS       DAZFAH010000219         6744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFAH010000219 DAZFAH010000000
VERSION     DAZFAH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.221"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..618)
                     /gene="sixA"
                     /locus_tag="ACHJWB_004200"
     CDS             complement(133..618)
                     /gene="sixA"
                     /locus_tag="ACHJWB_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4315733.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(821..2965)
                     /gene="fadJ"
                     /locus_tag="ACHJWB_004201"
     CDS             complement(821..2965)
                     /gene="fadJ"
                     /locus_tag="ACHJWB_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4315734.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINYALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGLGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2965..4275)
                     /gene="fadI"
                     /locus_tag="ACHJWB_004202"
     CDS             complement(2965..4275)
                     /gene="fadI"
                     /locus_tag="ACHJWB_004202"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4315735.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4455..4739)
                     /locus_tag="ACHJWB_004203"
     CDS             complement(4455..4739)
                     /locus_tag="ACHJWB_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4315736.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5111..6451
                     /gene="fadL"
                     /locus_tag="ACHJWB_004204"
     CDS             5111..6451
                     /gene="fadL"
                     /locus_tag="ACHJWB_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4315737.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1674 a         1817 c         1606 g         1647 t
ORIGIN      
        1 cgaatgaatg tagaaagtgt tttatttata gataaggaga gtcttcgata gtagtgaggt
       61 tgacagatat ccgctgcctg atgagcaatt atgcccaatg aatctacctc attgggcata
      121 atttggaaca catcagatag cttttgccat cttcagatta cacgggctca tctgccagtt
      181 gaatgtaccg tttccgcttt catcaagcgt tacgctggca atggccgagg tggtaaacat
      241 aggcggcgtt tcgcccggac ataactcggc aaccagatac ccgactaacg gtaagtgaga
      301 gatcaccagt accgaagcaa caccttcatt ggtcaacgcc tgcaaatagg cactgaccag
      361 accaacatcg ccgcagggcg ttaactccgg cagaacttct gcactggaag gcaggttcag
      421 acaatcccct acttcttcca gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg
      481 ttcgatttcc actttttgac ctttcagcca gttcgccatc aggcgagatt cgtcacaacc
      541 attagtggtc agaggacgaa ccgaatcact ggcggcatcg agggctgcgt cgccgtgacg
      601 catgataaaa acttgcatat tgcaccgctt ttgttaacca gattcgccca ttttctttca
      661 tctgaaacca gagaaagaaa acggtggtcg gcattgtgcc ttatccattc accgaatgaa
      721 acgctgtttt ttacctcaat ggcgtaagta tagtcaatat gcgtttacat tttgaccagc
      781 actccgccat tcagcgcgga ttcatatagc tttgaccttc ttattgcagg tcagttgcag
      841 ttgttttcca aaaactttcc ccacgtgcgc ccatctcgac caaacgctcg caaggggtaa
      901 aacgggaacc atactgcgtg gccagtcgtt gcattattgc aaccacttcg cccgcgccga
      961 gagaatcgat atagcggaac ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg
     1021 caccaatatc cccgtcacgc acgctacgga taacctgctc atcaacacaa cgtactgctt
     1081 cattcagcat caacatcaca caccgttcag caacctgcgg tgcggagagt cgccccagcc
     1141 cttgtgcgcc aatgagcggg taaatggcgg gatcgacctg ttttttgctt ttacgccctt
     1201 tctgaccata aagatagaaa ccccgaccat tttttctgcc tttgcgatcg tcgttcaaaa
     1261 ttgaagaaac aacatttgca ggcgcgctaa aacgttctcc ataagcggct tccagtacag
     1321 gaataatttt agtcccggtg tcgattccta cctcatccaa aagttggatt ggacctaccg
     1381 gaaaaccaaa tttcactagc gcggcatcaa tgtgctctac ccgttcacct tcggtcagca
     1441 tgcggatagc ttcattaatg taaggcgcta agatgcgatt gacgtaaaac ccggctttgt
     1501 cacgcacgac aatgggcgtt ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg
     1561 tttgcgctga tgtacccgca tgaggaataa tctccaccag cggcattttt tccaccggac
     1621 tgaagaaatg caggccgata acttgctcag gtcgcgtggc gtgagcggcg atatcaccaa
     1681 tcggtaaaga tgacgtattc gaagcaaaga tggtatgagc ggcgctattt tgctcaactt
     1741 ccgccaccat ctgttgtttc aattcgagat tttcaaacac cgcttcaata atcagatcgc
     1801 gatgggcaaa gccgcgatag tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt
     1861 cgctggcttt gagatgacga cggcgaactt tgccctccag ctgatcccaa ctgtacttca
     1921 gcgcataatt tatgccctga gggttgatat ctttaattct gaccggaagc cccgctttac
     1981 aagcagtgac ataagcaata ccgccgccca tcaagccacc acctaaaatt cccacgctgt
     2041 ttaatggcgc aggcggtgca tcactaccgg gatctttctt cacgtccgta ctggcaaaaa
     2101 agatactacg caaagcttgc gattgtggcg tcatcgccag ttcgccaaac gcccgagctt
     2161 cggcgtcata accgctgctg gtgccctgcg ctaatcccgt ttcaacaacc tccagaatgc
     2221 gttctgtcgc cggataattg ccttgagttt tgtgttctgt tttcttgccg accattttga
     2281 acagcagcgc acgacctaac ggccccgcca gaatacgctc gcgtacaggt agagggcggg
     2341 aagatgggcg atccttcttc gccagctcaa cagcgacttc cagcaaaatg gagtgcggaa
     2401 caacgtcatc caccagcccc agctttaatg cctgtttcgc ccgaagttgt tttccggtga
     2461 ggatcatctc taatgctgtg ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg
     2521 aaccgggtaa caatccaagt tgtacttcag gcaaaccgag caccgtttta ggatcgtcag
     2581 tacaaacgcg accgtggcac gccagcgcca actccagccc accacccagg caagcgccat
     2641 gaatagccgc gataaccgga atgggcaaag catgaatctc cgccatcaac tgttggccct
     2701 gccgcgccag tgcttccgct tcttgcgccg ttttgcagtt gccaatcatg ttgatgtctg
     2761 ctccggcgat aaaattgtcc ggtttcgcgg agataaacac cacgccgcgc aactctttgt
     2821 tttcacggag ttgcttaata atggcgcgca cctgcgaggc aaattccgcc ttcagggtat
     2881 tcattttctc atccggtacg tcgatggtga tgatagcaat gttgtccaga cgaacattaa
     2941 gagtaaacgc tgatgccatt tccattattc cgcctccaga accattgccg cgccaagccc
     3001 accggcagca caggcggtaa ctaaaccaaa tccaccgccg cgacggcgaa gttcatgcaa
     3061 tgtctgggta atcatccgcg cgccggtcgc cgcgaaggga tgtccgtaag caatcgaacc
     3121 gccaagcacg ttaaatttgc tatcgtccac ttcgccagtg gcatgtgcac gccccagtac
     3181 ttcacgagca aaacgttcac tacccagcaa ctgaatattc gccagtgtct gagctgcaaa
     3241 ggcttcgtgc atatcgatca atgtcagttc gctcatcgtc aaaccggcac gttccagcgc
     3301 cagcggtgtt gaccaggctg gaccgagcaa catgtcctgc cagacatcaa tcgcagtaaa
     3361 tgcgtagctg cgcagatacc ccagcggcac cagccctaat tctttcgccc gggattcagt
     3421 catcaggatc accgctgccg cgccatcggt cagcggcgta ctgtttgccg ccgttaccgt
     3481 tccgtgtttg cgatcaaacg ccgggcgcag ctttgcgtaa tcggcaagcg aggaattacc
     3541 gcgaatattg ttgtcttcga caagcggttg tttataagga gggataaagg cagtcatcac
     3601 ctcttctttg agttttcctt ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc
     3661 taatgcatct tgctgttctc gggtgatgcc gtaggttttc gccatttgtt cggcggtgtc
     3721 gcccatccgc aagccagtag aatattctgc taccgcaggc ggtacgggca ttaagtcgcg
     3781 caaacgcagg cgagagaaga gtttcaggcg ctggctcatg gttcgagctt tgttgacatc
     3841 aaccagcacg cgcgccagtt ttttactgac gccaattggc aatacggaag aggaatctgc
     3901 cccaccggca atccccgctc ggatagttcc cgccatcagg ctttctgcga cgtttgcaac
     3961 cgcctggaaa ctggtagcgc aagcgcggct gacgctgtaa gcatcggtat gcacattcat
     4021 tccggtaccg agaacaattt cacgcgcaat gttgggggct tcaggcattt gtacgacctg
     4081 accaaagacc agctgttcaa tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc
     4141 taccaccatc ttccctaaat caaccgcggg aatgccatga aaagccgtcg cctggcgggc
     4201 aaaaggcgta cgtaaaccgc taacaatggc gatacgatcg ccctggcggg taaccagcgg
     4261 taaaacctga cccataacac tcccctgtaa aaaaataaat aaagtggtct gacctgatca
     4321 tagtcttaac cattttttta catttagcca agcggagaaa aaagaaagtg ggagctatga
     4381 cacagtgaag acaggagaaa gaaaagaaaa cgcccctgcc agcaactgac aggggcgaac
     4441 gtacccgaga ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca
     4501 aaagtgaaat cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggagatt
     4561 ttgcacggtt cggattccac gctacgggct ttttcagtca gcgccgccag cgtttgttct
     4621 gcttctgcgc ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata
     4681 atggtgccaa catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc
     4741 gttgatttcc tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct
     4801 tccggaaagt gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca
     4861 cttaaaaatg atctaaaaca aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt
     4921 gccagctgga tcgcgtttct tagatcatat ttgaaaaaag atggaaacat acttgcaaca
     4981 ttccagctgg tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac
     5041 cctacacttc gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga
     5101 ggttatggtc atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc
     5161 acttatctcc acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg
     5221 cctgggccgg gcttattcag gggaaggcgc aattgccgat gatgcaggta acgttagccg
     5281 taaccccgca ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat
     5341 tgacccggat gtgaatatca gcggaacgtc tccgtctggt cgtagcctga aagccgataa
     5401 catcgcgcct acggcatggg ttccgaacat gcactttgtt gcaccgatta acgaccaatt
     5461 tggttggggc gcttctatta cctctaacta tggtctggca acagagttta atgatactta
     5521 tgcaggcggc tctgtcgggg gtacaactga ccttgaaacc atgaacctga acttaagcgg
     5581 tgcgtatcgc ttaaataatg catggagctt tggtcttggt ttcaacgccg tctacgctcg
     5641 cgcgaaaatt gaacgtttcg caggcgatct ggggcagttg gttgctggtc agattatgca
     5701 atctcctgcc gggcaaactc ctcaagggca agcattggca gctaccgcca acggtatcga
     5761 cagtaatacc aaaatcgctc atctgaacgg taaccagtgg ggctttggct ggaacgccgg
     5821 aatcctgtat gaactggata aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa
     5881 aattgacttc aaaggtaact acagcagcga tcttaatcgt gcgtttaata actacggttt
     5941 gccaattcct accgcgacag gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc
     6001 tgaaatgtgg gaagtgtcag gttataaccg tgttgatcca cagtgggcga ttcactatag
     6061 cctggcttac accagctgga gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga
     6121 cacgctgttc cagaaacatg aaggctttaa agatgcttac cgcatcgcgt tgggtaccac
     6181 ttattactac gatgataact ggaccttccg taccggtatc gcctttgatg acagcccagt
     6241 tccggctcag aatcgttcta tctccattcc ggaccaggac cgtttctggc tgagtgcagg
     6301 tacgacttac gcatttaata aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg
     6361 tcagagcgtg aaaattaacg aaggcccata ccagttcgag tctgaaggta aagcctggct
     6421 gttcggtact aactttaact acgcgttctg ataacgcgtt cgcctggata aagtcacctg
     6481 catagcaggt gactttaact cccccacttc accgaaagta gtgtcctcat tgcttacctc
     6541 ctgagttttg caaacagcct gttggcagct tgccctttca aatcaataag cggtatccac
     6601 agacaaacct gaagggaaag gcatattttc aggcgttctg ctcgtccttc tcaaagagtt
     6661 tacttttctg catttccagg atactccccc cctggctatt gtgcgctcat acactcaaat
     6721 taaagatagg ttctaaataa atga
//
LOCUS       DAZFAH010000220         6577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFAH010000220 DAZFAH010000000
VERSION     DAZFAH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.222"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..491
                     /gene="ykgH"
                     /locus_tag="ACHJWB_004205"
     CDS             <1..491
                     /gene="ykgH"
                     /locus_tag="ACHJWB_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO4315738.1"
                     /translation="GILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIRLE
                     WSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLT
                     RNWPLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMEDEYQQNS
                     GQ"
     gene            complement(734..1429)
                     /locus_tag="ACHJWB_004206"
     CDS             complement(734..1429)
                     /locus_tag="ACHJWB_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO4315739.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     KSSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1422..2849)
                     /locus_tag="ACHJWB_004207"
     CDS             complement(1422..2849)
                     /locus_tag="ACHJWB_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO4315740.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2860..3579)
                     /locus_tag="ACHJWB_004208"
     CDS             complement(2860..3579)
                     /locus_tag="ACHJWB_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO4315741.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKIVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4106..4960)
                     /gene="rclR"
                     /locus_tag="ACHJWB_004209"
     CDS             complement(4106..4960)
                     /gene="rclR"
                     /locus_tag="ACHJWB_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO4315742.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5186..6511
                     /gene="rclA"
                     /locus_tag="ACHJWB_004210"
     CDS             5186..6511
                     /gene="rclA"
                     /locus_tag="ACHJWB_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO4315743.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1782 a         1627 c         1441 g         1727 t
ORIGIN      
        1 caggtatact tgttagctgt gatagcaata ataattttgc ctgtcagacc acaatgactg
       61 aagatgttat tcagcgaatt actacatttt ttcagaccag cccagatgtc aagaacagag
      121 aaataaggct ggaatggtca ggagataaga gagctttacc aactgctgaa gaggaaatat
      181 ctcgcgtgca ggcctctatt atcaaatggt atgcgtcaga atatcataat ggcaggcaag
      241 ttctcgatga gatacaaacg ccttcagcaa ttaacagtga gctttataca aaaatgatat
      301 acctgaccag gaactggcca ttgtatccga acggtgatgg ctgtgtaact ataagctcac
      361 cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca
      421 ttgtaatttc tgtaatgttt tgccttgtca aaaaaatgga agatgaatac caacaaaact
      481 ctggacagta ataaacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg
      541 accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt aaatatcctg
      601 gcattactaa tgccaggata tttacagaag agcagcataa ccatgacgga acggggttaa
      661 gtcaggttgt ccttaacgct attttcaacc tcgtatgcct tcttcaggtt tatgttcaga
      721 tctcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcact ggaccatgaa
      781 ccccgacgac tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa
      841 tgcaggacgg cattcgttca ccggcctgcg ctttctgatg caatttttct gcgagttgcg
      901 ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagatttc gggagcaggc
      961 tcaatgaacg cccgcgctcg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
     1021 catattcagc atacacgaca cccactttag cctgctctgc ctgcgagata ttctcggcac
     1081 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttca ctaatcccca
     1141 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
     1201 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa
     1261 catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc
     1321 gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
     1381 gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc
     1441 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
     1501 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
     1561 aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta
     1621 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca
     1681 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg
     1741 ctgtcacaag cagtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
     1801 ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
     1861 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc
     1921 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
     1981 tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg
     2041 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
     2101 tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt tttaggcagc
     2161 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc tgtctctgcc
     2221 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
     2281 tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttca
     2341 tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagat
     2401 ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatg
     2461 ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga tttagatttc
     2521 accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct
     2581 ttggtttttg caaaccagac gtgaccgccg ttttgcgtca ctttttctga gagctggtac
     2641 agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac
     2701 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
     2761 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
     2821 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc
     2881 agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac tgatgttcag
     2941 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcacaat
     3001 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
     3061 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
     3121 cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata
     3181 cacagctctc ccttgcaaac tggcacctac atcgactacc cctaatttat taacaataaa
     3241 agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc attcaggttc
     3301 atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat
     3361 aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
     3421 tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
     3481 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
     3541 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
     3601 tgcccattta tgcccgatgt ttccccccca ccgggggatc aatcatcaat aaatatggcg
     3661 ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat gataatcttt
     3721 catccaaacg tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
     3781 gggaaatcga cgatcatttt tggcataaga aatccattta aaaatggcta catcccttat
     3841 acctattcag agctacttat tcaggggtta ttgagatata cactgatggt gtacaaaaaa
     3901 atcaccgaca ataatcacgt atcaagtaat agtcgggatt ttatttaatt gaagcaattt
     3961 agtttgcatt tttatttaaa aactgagcta gagtccatca gaaaaaatat ataaagccca
     4021 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
     4081 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
     4141 ctcccggggt acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat
     4201 agcctactga ctcagcgatc accacaacag ggagcgtttc ccgggaaaac atctgggccg
     4261 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
     4321 aaagctgggc aaaacttgcc cgggacatgt gagcgatgct ggccagcgat tcgacggtcc
     4381 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
     4441 gcaaactgag aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg
     4501 taaagaatgt agcgcagatc tggctacaca gtgcatccac tcccggcatt gccgatctgg
     4561 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
     4621 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
     4681 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
     4741 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tcccccgtcg
     4801 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
     4861 cggcaccatg tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
     4921 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
     4981 ttgattacaa acaccaacaa tcttagtaaa taactaatct aataccatca aatagttaat
     5041 catgattttt gttttgtgat tctgattaag aaaaagaaaa tatcagacga taagacaaaa
     5101 tatcaagcgt tgtgaagaaa tgttatttgc tcttttgcgt ctatagtcat gatgtcaaat
     5161 gaacgcgttt cgacaggaaa tcatcatgaa taaatatcag gcagtgatta ttggttttgg
     5221 caaggctgga aaaacattag ccgtcacgct ggcaaaagca ggttggcgtg tggctctcat
     5281 cgaacaatca aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa
     5341 aacattggtt catgacgcac agcagcacac agattttgtc cgagccatac agcgtaaaaa
     5401 tgaagtggtt aattttttac gtaataagaa ttttcataat cttgcggata tgcccaatat
     5461 cgacgtgatc gacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc
     5521 tgaggcaaat ctggaaattc atggcgagaa aatttttatt aataccggtg cacaaaccgt
     5581 ggttccgcca attcctggaa ttaccaccac gccaggagta tatgacagca ccggattact
     5641 taatctaaaa gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga
     5701 gttcgcctct atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct
     5761 gtttttgcct cgggaagatc gggatattgc tgataatatc gcgacgattt tacgcgatca
     5821 gggcgtcgat attatcctca atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt
     5881 gcaagtgcat agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg
     5941 tcaaccggct accgcttcgt tacatccaga aaatgccggt atcgcagtaa acgagcgcgg
     6001 ggcaattgtc gttgacaagc aattacatac caccgcagac aatatttggg cgatgggaga
     6061 tgttaccggc gggctgcaat ttacttatat ttcactggat gattaccgca ttgtgcgtga
     6121 tgagttgctg ggagaaggca gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt
     6181 attcatgaca ccgccactgt ccagggttgg tatgacagaa gaacaggcca gagagagtgg
     6241 tgctgatatt caggtggtga cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa
     6301 tgatactcgt ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc
     6361 atcactgctg tgtgttgact cccacgagat gatcaatata gtgaaaatgg tgatggatgc
     6421 cgggctgcct tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc
     6481 actcaatgat ctattttcat tagtcaaata aactcataat cagacgccag aacaaatatt
     6541 ctggcgcctc agagaaaaga atcttattaa ttctctg
//
LOCUS       DAZFAH010000221         6573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFAH010000221 DAZFAH010000000
VERSION     DAZFAH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.223"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..713
                     /locus_tag="ACHJWB_004211"
     CDS             150..713
                     /locus_tag="ACHJWB_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315744.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            795..3437
                     /gene="yfcU"
                     /locus_tag="ACHJWB_004212"
     CDS             795..3437
                     /gene="yfcU"
                     /locus_tag="ACHJWB_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO4315745.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKTGQLEGMEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQLQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDSAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3457..4209
                     /locus_tag="ACHJWB_004213"
     CDS             3457..4209
                     /locus_tag="ACHJWB_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4315746.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4184..4732
                     /locus_tag="ACHJWB_004214"
     CDS             4184..4732
                     /locus_tag="ACHJWB_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315747.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4729..5199
                     /locus_tag="ACHJWB_004215"
     CDS             4729..5199
                     /locus_tag="ACHJWB_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315748.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5196..5720
                     /locus_tag="ACHJWB_004216"
     CDS             5196..5720
                     /locus_tag="ACHJWB_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315749.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSAAGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5707..6555
                     /locus_tag="ACHJWB_004217"
     CDS             5707..6555
                     /locus_tag="ACHJWB_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4315750.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGRG
                     AYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQPIDSTCRFQ
                     YVLNEL"
BASE COUNT         1738 a         1478 c         1669 g         1688 t
ORIGIN      
        1 ggccccacac caacgaatac aattttctac gacctgttct ttttggatcg catgccgtat
       61 tcaatggctg ggtataaaca ttggctattg actgaatcaa tgtattgcaa tgtttatatt
      121 tttaattatt tatagtttaa ggacaaataa tgagtaagtt tgttaaaaca gctattgctg
      181 cggcaatggt gatgggcgtg ttcacctcta cggcaacaat cgctgcgggt aacaacggta
      241 ctgcacgttt ttacggcacc attgaagact cagtgtgttc tatcgttccg gacgatcaca
      301 aactggaagt tgatatgggt gatatcggtg ccgaaaaact gaaaaataac ggcaccacca
      361 cgccgaaaag tttccagatt cgtctgcaag attgcgtatt tgatactcag gaaacaatga
      421 ccactacctt tactggtacc gtttcttctg caaatagcgg caattattac accattttca
      481 ataccgatac cggtgcggca tttaacaatg ttagcctggc gatcggtgac tctctgggta
      541 cctcttacaa aagcggcatg ggtattgacc agaaaatagt gaaagacact tctaccaata
      601 aaggcaaagc gaagcagaca ctgaacttta aagcctggct ggtcggcgca gctgatgcgc
      661 cagatctggg taatttcgaa gcaaatacca ccttccagat tacttacctg taaggcatac
      721 ccccatagcg acactgctat gggggattta aatggatttt gaccgaacaa gaataaaaac
      781 ggatagaaac gtgtatgcct gaccattctc tttttcgatt acggattctt ccgtggtgca
      841 ttgcgctggc aatgtcaggg agttatagca gtgtctgggc tgaagacgac attcagtttg
      901 attcccgttt tctggaatta aaaggcgaca caaaaattga tctgaagcgt ttttccagcc
      961 agggatatgt tgagcccgga aaatacaatt tacaggttca actaaataaa cagccattgg
     1021 cggaagagta cgatatttac tggtatgctg gtgaagatga cgcgagcaaa agctatgctt
     1081 gtctgacacc ggaactggta gcgcagtttg gtttaaaaga agacgtggcg aaaaatctgc
     1141 aatggagcca cgatgctaaa tgcctgaaaa ccggtcaact ggaaggcatg gaaattaagg
     1201 ctgatttaag ccagtccgca ttagtcattt cactaccgca ggcttacctc gaatatacct
     1261 ggcccgactg ggatccgcct tcacgttggg atgacggcat ttccgggatc gtcgcggact
     1321 acagcatcaa cgcacaaacc cggcacgaag aaaatggcgg tgatgatagt aacgagatca
     1381 gcggcaacgg gacggtcggg gttaacctgg ggccgtggcg tatgcgtgct gactggcaga
     1441 ctaactatca acatactcgc agtaatgatg atgatgaatt cagcggcgat gaaactcaaa
     1501 aaaaatggga gtggagtcgc tactatgcct ggcgggcgtt accatcatta aaagccaaac
     1561 tggcgctggg cgaggattac ctcagatccg atatttttga tggttttaac tatgttggtg
     1621 gcagtgtcag tactgacgat caaatgttgc ctcccaatct gcgcggctac gcgccagaca
     1681 tttccggcgt ggcacacacc acagcaaaag tgaccgtcag ccagatgggg cgtgtgattt
     1741 acgaaacgca ggttccggct ggaccgtttc gtattcagga tcttggtgat tctgtctccg
     1801 gtacgttgca tattcgcatt gaagaacaga acggccagtt gcaggaatat gacatcagca
     1861 ccgcctcgat gccatacctc actcgtccag gtcaggttcg ctataagatc atgatgggcc
     1921 gtccgcaaga gtggggacac catgtcgagg gtgaattttt ttctggtgct gaagcttcct
     1981 gggggatcgc taacggctgg tcgttatatg gcggcgcact aggagatgaa aactatcagt
     2041 ctgcggcgct tggcgtcggt cgcgatttgt ctacattcgg cgcggtcgcg tttgatgtta
     2101 ctcactcgca caccaaactg gataaagaca gcgcttatgg caaaggttcg ctggacggta
     2161 actccttccg tgtgagttat tccaaagact ttgaccagct caacagccgc gttacttttg
     2221 ctggatatcg cttctcggaa gagaacttta tgaccatgag cgagtatctg gatgccagtg
     2281 acagcggaat ggtacgcacg ggcaacgaca aagagatgta caccgccact tataaccaga
     2341 acttccgcga tgcgggtgtt tcggtttatc tcaactatac ccgccatacc tactgggatc
     2401 gcgaggagca gacaaactac aacatcatgc tctcgcacta tttcaatatg ggcagtattc
     2461 gtaatgtcag catctcgatg actggctacc gttacgagta tgacaaccag gccgacaaag
     2521 gcatgtacat ttcgctcagt atgccgtggg gcgacaacag taccgttagc tataacggta
     2581 actatggcag tgggacggac agcagtcagg tcggttattt cagccgtgtc gatgacgcga
     2641 ctcactatca gttgaacgtc ggcaccagtg acaaacacac cagcgttgac ggctattaca
     2701 gccatgatgg ttcgctggcg caggttgacc tcagtgcgaa ctaccatgaa gggcaataca
     2761 cctctgcggg cttgtcgtta cagggcggcg caacgcttac tgcccacggt ggcgcacttc
     2821 accgtaccca gaatatgggt gggacacgct tgttgattga tgccgatggc gttgccgatg
     2881 ttccggtaga aggtaacggt gctgctgttt ataccaatat gtttggtaaa gccgtcgttt
     2941 ctgacgtcaa taactattac cgcaatcagg cgtatatcga cctcaacaaa ctgccggaaa
     3001 acgccgaagc aacccagtcg gtggtacaag ccacgctaac tgaaggggcg attggctacc
     3061 gcaaatttgc cgtcatcagt ggtcaaaaag cgatggcggt gctgcgttta caagacggca
     3121 gccatcctcc gtttggcgca gaagtaaaaa atgataacga gcagacagtg ggccttgtcg
     3181 atgatgacgg caatgtttat ctggctgggg tgaaacctgg cgaacacatg agtgtgttct
     3241 ggagtggtgt tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc gatctgttta
     3301 acggcttgtt actgccatgc cagcataaag gcaacgtcgc gccggttgtt cctgatgaca
     3361 taaagcctgt cattcaggag cagacgcaac aggtgacacc caccaatccc cctgtttctg
     3421 tttcagctaa ccaataacgt gattaaggaa tgatccatgt ctgatttact ttgctctgcc
     3481 aaattgggtg ctacgacgct agcgttactg cttagcgctg ccagtttgtc tgcccaggct
     3541 tctgtaacgc cagatcgtac ccgtttgata tttaatgaaa gcgacaaatc aatcagcgtg
     3601 acgttgcgca ataacgatcc gaaactgcct tatctggccc aaagctggat cgaagatgaa
     3661 aagggtaaca agatcagttc accgttaact gttctgccac cggtgcaacg catcgattca
     3721 atgatgaacg ggcaggtaaa agtgcaggga atgccggata tcaacaaatt gcctgccgat
     3781 cgtgaaagcc tgttctactt caacgtgcgt gagatcccgc caaaatcaaa caaagccaac
     3841 acgctgcaaa tagcactaca aacgcgtatt aagttgttct ggcgaccgaa ggcgctggag
     3901 aacgtcagca tgaaaaaccc ctggcaatat aaggttactt taacccgcaa cggtcaggaa
     3961 tttaccgtca ataacccgac accgtattac gtcattttca gtaatgccag cacacagaaa
     4021 aatggcaatc cggcagccgg tttctcgcca atagtgatgt cgccgaaaac ctctgcaccg
     4081 ctgaatgtga agatgggtag cgtgccggta ctgacctatg tcaacgacta tggcgctcgg
     4141 atgccgctct ttttcacttg caacggtaac agttgccagg tcgatgaaga gcagagccgc
     4201 aaaggctgag gccgggagag ccagtatgaa aggatcttgt tctgtctggg cggtaactgg
     4261 gttactgata tgttgtgttg gtagcggctt tgcagttgca gcgagtaagt cagtaagtat
     4321 gaatttacgg gtgcttgtcg atgcccctcc tccttgtacg gtaaatggtg cggcagttga
     4381 gtttggtaat gtattcatta ataagatcaa tggggttgat tataaacgtc ccattgacta
     4441 ctcgcttgtt tgtaacaatc tggcaatgga tgacctgcgt ctacagatgc aggcgacaac
     4501 ggtcgtgatt aacggtgaaa cagttatcag cacggggatt cctgggtttg gtattcgggt
     4561 tcagaaaagt agtgatcaca caatactcga cttaacctct ggctcatggt tgccatttaa
     4621 tttttcctcg ggagttcccg ttctggaagc tgtgccggtg aaacagagtg gtacaacgtt
     4681 agcggcagct gaatttaacg ccagtgccac aattgtggtg gattaccaat gaaacggatc
     4741 tcgcttattt tgctgtgggg cttttgctcg atggcactct caaacgtctc ttttcacggt
     4801 tatttagtgc agccacctaa ctgcacgatt agcaacgctc aaaccattga aataactttt
     4861 caggatgtgc ttattgatga cattaatggc agcaattatg agcaaaccgt gccgtattcc
     4921 attacctgcg atacggcggt tcgcgaccca ttaatggaaa tgaccttgag ctggagtggc
     4981 acaccaagcg attttgataa tgctgcagta agcagcaata tcactggttt aggcattcag
     5041 ctcaaacagg cagggcagag ttttacgatc aatacgccac ttgtggtgaa tgaaaccgat
     5101 ttaccggtgt taacggctgt gccagtaaaa aagagtggtg tcgttctgcc agaggcggac
     5161 tttgaggcct gggccacgtt acaggtagat taccaatgaa gaaaaagcgt acgttgtttt
     5221 ttattagtag cctgatgttg ctgggcagtg gaaccacaat agcaggagat aatttgcact
     5281 ttaccggcaa tttaatcagt aaatcgtgta cgccagtgat taatggtagc cagttggcag
     5341 aagtacattt tcctgccatt gccgccagcg atttaatgaa ccttgggcag tcagagcgag
     5401 tgccattggt gtttcaattg aaagattgtc atagttctac attgtttaat gtcaaagtaa
     5461 cgttgacagg aacagaagat agtgctctac ccggctttct ggcctttgac tcctcgtcat
     5521 cagcagccgg cgctgggatt ggtattgaaa ctgcggcagg cacatcggtg ccaatcaaca
     5581 atacaacagg cgtcacgctc ccgctgaatc agggaaataa cagcctaaat tttaatacct
     5641 ggttacaggc aaagagcggg cgagatgtaa cgtccggaga ctttagtgcc acagtgacgg
     5701 caacctttga gtatttctga aatgaaaaaa tggaccataa ttttcgcgag cctaatgctg
     5761 ctcgttctga gtgttgtcgg cgcatctaag agttatgctg gagataaact tatgagtgcg
     5821 agttttgaca gtgtgagagt ttattatgcc atggacaagg taaccggtgc tgtagcgagc
     5881 agtgttttca atgttacggt gataacgcca aaggaggttg catatgggaa atatgattct
     5941 tttgcgtata aaggcgatac gttgcgggtt atttcctggt ctgggtcggg ttcagctcca
     6001 actttggtac tgactgactt tgatacaatt aataactcaa attgccctgg catagatacg
     6061 aaaatttttc gttgtgcata tatgacgttt aaaattaccg ttgcatctga tgattatggt
     6121 tgcccttgga tcgcatcctt ttattcctat acagatttac ccggatttgg aagctatacg
     6181 gcccctacag tgcataacac aatatgccca acgataccgg tcgcttcata cgatatctcc
     6241 tggagtgaaa attatgtcag ccataataag gcgttacgaa ttcagtccac ggggtcaaca
     6301 gtgaccacca cgctttcgac ctacttgatg gaaggcggca gactctgtga tggcagagga
     6361 gcatactgcc gcgcggtatc agaattgtta acctttacct cttacggttg tgataaatca
     6421 acggtaacgg ttacgccaac tcgtcatccg gtaacggata aagtactaca cgatattgtg
     6481 gtgaatgtga atactagtag tgggcaaccg attgattcga cgtgtcgttt tcagtatgta
     6541 ttgaatgaac tgtagttcgt agtattgctc tga
//
LOCUS       DAZFAH010000222         6512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFAH010000222 DAZFAH010000000
VERSION     DAZFAH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.224"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..2380)
                     /gene="rhsC"
                     /locus_tag="ACHJWB_004218"
                     /pseudo
     CDS             complement(1..2380)
                     /gene="rhsC"
                     /locus_tag="ACHJWB_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
     gene            2609..3217
                     /locus_tag="ACHJWB_004219"
     CDS             2609..3217
                     /locus_tag="ACHJWB_004219"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4315751.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKQVENLFSRESFARTEPPKA"
     gene            3315..4706
                     /gene="selA"
                     /locus_tag="ACHJWB_004220"
     CDS             3315..4706
                     /gene="selA"
                     /locus_tag="ACHJWB_004220"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4315752.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            4703..>6512
                     /gene="selB"
                     /locus_tag="ACHJWB_004221"
     CDS             4703..>6512
                     /gene="selB"
                     /locus_tag="ACHJWB_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4315753.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT         1311 a         1799 c         1941 g         1461 t
ORIGIN      
        1 tccacggcgg gcaggctgtc cggtatacag cggttcgcca gtccctgcgc gttgtacgca
       61 tgtctggtct catgctgcca gagcagttca ttcgtctgcg gatgatgcac cgtcaggtgt
      121 tcactggcga ggcggccttt actgtcataa ccatagtgca ccgccacccg gtgcccttcg
      181 ctgatatggc tgatgtctgt cagccagccg cgttcgtcat actgccactg ctctgcggtt
      241 tcgccattca cggtgcggtg cgtgaggcgg tctgcttcgt catagtgcca gtgggtgacc
      301 agcccctcat cctcgctgcg gataagtttg ccggtcaggt cgtggtgata acgctgtgtg
      361 cggccgtcaa agccggtttc ctgtatcagc cggtcgagta catcgtaacg gaaggtggtg
      421 tggctgccgt tttcactggt caggcggatg acccgtccgg cagcatcgta ttccatactg
      481 cgcgtcagac cgccctgcgt ggtacagatg gcttttcccc acgcatcgta ctgtgtcccg
      541 tttctgctgc cgtccggggc aatgacggtg gtcaggtcac cggcggcgtt gtattcatac
      601 cgcgtttcat ggccctgcgt gtctttcacg gcaattaact gtccacggct gtcgtatgcg
      661 tggtactggc tcaggccttc ctcgcggtgc accgccgtca cctgaccaaa acggtcatgg
      721 tcatagcggg ttacataacc ggaacagtca gtgaaggtca gcaactgtga ccgtaacggc
      781 tccacgtcat ggttttccgg ctgccggtgg catcttccgt tgcgcagggg aggtcactgt
      841 gtggattatc ataacggtag cgggtgatat cgccgtcagg ggcggtttcc tgaatcagac
      901 gtccgtactc atcatattcc cggcgcaatt ccagcccgtc tggcccggtg gctgacgtta
      961 actggctgtg gtggttatag taaaacgccg atgccctgcc atccggcgtg gtgatgcgcg
     1021 tgatgaggcc cgtcaccaca tccgggctgt actctgttgt cctgcctgcg gcatccgtct
     1081 gtgccttaag cctgcccacc gcatcaaact gactctgcgt gacgctgccg tccgcgtgtt
     1141 cctttttcac cacccgcttc agcccggctt caccctgcgt gtgcagcact tcacggcggt
     1201 tcaggctgtc ggtgatggtg atgcggtcct tctcatactg atacgtgtag cttaagcctg
     1261 ccgggtttag ctgttctgtc acccgcccgt cgctgtcgta acggtaacgg atttccggtc
     1321 ggcccgtgtg acggtgcgcc accatccggc cccggtattt atcatcgtaa gtaaagctgc
     1381 gcacctgtgt attgctgcgg tcatacactg ccgccagttc gccgcgcggc gtccagccat
     1441 agcgcaccag cggcgcggca ggtaaattct ccgggtattc cgggtcgtgc gtcagccaca
     1501 cggcagacag acggatgccg ttgtcccggc catattcggt gtaacccggc agggtatcag
     1561 gaaaagcgga cggttccgtc ccgccggaaa tggcctgctg ccgggcttct tctgcccgct
     1621 gcgcctgcgt ggtcagtacc agccggaagt gacgcccggc accatccgtc acgccggtga
     1681 tttcgccgct gaattcaccg gcggcttcgc ggtggaacgt ctgtgtgcgc ccgaagcggt
     1741 ccaccagccc ggtcagtacc cggtacggcg gcagcggcgc aggcagcacc tcatccgctt
     1801 ccggcacccg ctcacaccag ccgagcagcc accacggccg ctgcggactg tttgtcgcca
     1861 gataacgatg cggacttaag cggagttctt ccggcagcgc ctgccagagt gcggccagcc
     1921 ggtgaccttc atccagtttc gccacgccgc cgcgcaccag ccacagtgac tcgctgcggc
     1981 tgtaaccgtc ctcaccggga aacaggtgct caaaatacag gcttctgccg ccgttatcac
     2041 tgagtatcag tgtgttatcg cgcagctgta agcggatatc cgcaggcatt ttccagccgg
     2101 ggtcgaggct ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtgcgggaga
     2161 ggatgaacgg cagcggggcg ggcagggcga tgtcggtttc accgggaagg acctttgcac
     2221 cgagcagcgg attgaccgga tggccggacg tcaccccgcc ggggcacacc gaacaggcca
     2281 cgccggtggg ggcaccgatg cgtaccccgg ctgaaccctg gacaatgcta ccgccatact
     2341 gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat ctgctcatcc ttgttttatc
     2401 gggttatttg tctgcttcca ggcaaaaccc tacagattta taagaaagcc ttaagtgaga
     2461 tatccatcag atagaaacaa ttaggaaaat atttatatca ccatgataaa tatgtgattt
     2521 atttggaagt aacaaaccat caacattttc tgcccttttt cccatcacca ggccatactg
     2581 atatttttcc tcgcacaaag gactcggcat gaaactcgtc ggtagctaca ccagcccgtt
     2641 tgtacgcaaa ctttctattc tgttgttaga aaagggcata actttcgaat ttattaatga
     2701 actgccctat aacgcggaca acggcgtggc gcaatttaac ccgttaggaa aagtgccggt
     2761 gctggtgacc gaagagggcg aatgttggtt tgattcgccg atcatcgctg aatatattga
     2821 attaatgaat gtcgctccgg cgatgttgcc gcgcgatccg ctggagtcgt tgcgggtgcg
     2881 caaaattgag gcactggcgg atggcattat ggatgccggg ctggtatcgg tgcgtgaaca
     2941 ggcgcgtcca gcggcgcagc agtctgaaga tgaattgtta cgccagcggg agaaaatcaa
     3001 ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc acactcaaaa ccgatacggt
     3061 caatctggcg actatcgcca ttgcctgtgc cgtcggatat ctcaatttcc gccgcgttgc
     3121 gccaggttgg tgtgtcgacc gcccacactt agtcaaacag gtcgaaaacc tgtttagccg
     3181 cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc gcgatatgtc ctcctgaccc
     3241 atctcacgtt acaatccgtg gttatgttaa acgcccttct ccgtgtgaga gggccttgat
     3301 cagccaggtt tcctatgaca accgaaacgc gttccctcta tagtcaactt ccggctattg
     3361 atcgcttatt gcgcgatagc tccttccttt ctttgcgtga tacttatggt cacacccgcg
     3421 tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga agtgattcgt ggcagccaga
     3481 cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga tgcccggttg acgaaagaag
     3541 cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac cgtgctgcat accaaccttg
     3601 ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc gcaggctatg cgttcgccag
     3661 tgaccctcga gtacgatctg gacgacgccg gacgcggaca tcgcgatcgg gcgctggcgc
     3721 agctgctgtg ccgtattacg ggggcggaag atgcctgtat cgtcaataac aatgcggcgg
     3781 cggtgttatt gatgttggcg gccactgcca gcggaaaaga ggtggtggta tctcgcggcg
     3841 aactggtgga gattggcggc gcgtttcgta ttcccgatgt tatgcgtcag gcaggctgca
     3901 ccctacacga agtagggacc accaaccgca cgcacgcgaa tgattatcgt caggcggtga
     3961 atgaaaatac cgcactgttg atgaaagtac ataccagtaa ctacagcatt caggggttca
     4021 ccaaagcgat agatgaagcg gaactggtgg cgctcggcaa agagctggat gttcccgtag
     4081 tgactgattt aggcagtggc tcgctggtcg atcttagcca gtacggtttg ccgaaagagc
     4141 caatgccgca ggagttgatt gcggcgggcg tcagtctggt gagtttctcc ggcgacaagt
     4201 tgttaggcgg gccgcaggca ggaattattg ttggtaaaaa agagatgatc gcccgcctgc
     4261 aaagccaccc gctgaagcgt gcattacgcg cggataaaat gaccctcgcg gcgctggaag
     4321 ccacgttgcg tctttattta caccctgaag ctctgagtga aaaattaccg accctgcgcc
     4381 tgcttacccg cagcgcagag gtcattcaaa tccaggcaca acgtttacag gctccccttg
     4441 ccgcacatta cggcgcggag tttgcggtac aggttatgcc atgtctttcg cagattggca
     4501 gtggttcgct gccggttgat cgcctgccga gcgcggcatt aacgtttaca ccccatgatg
     4561 gacgcggtag ccaccttgag tcattagccg cccgctggcg tgaattgcca gtgccggtga
     4621 ttggtcgtat ttatgacgga cgattgtggc tggatttacg ctgccttgaa gatgagcaac
     4681 ggtttttgga gatgttgttg aaatgattat tgcgactgcc ggacacgttg accacggcaa
     4741 aacaacctta ttgcaggcga ttactggcgt aaatgctgac cgtctgccgg aagaaaaaaa
     4801 gcgcggcatg accatcgatc tcggctatgc ctactggccg cagccggatg gtcgcgtgcc
     4861 tggttttatc gacgtacccg gtcatgaaaa gtttctttcc aacatgctgg cgggcgttgg
     4921 tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac ggcgtgatgg cgcagacccg
     4981 tgagcatctg gcgattttgc agctgaccgg taacccgatg ctgacagtgg cgctgaccaa
     5041 agccgatcgc gtggacgaag cgcgtgttaa tgaggttgaa cgccaggtaa aggaggtgct
     5101 gcgggaatac ggttttgctg aggcaaaact gtttatcacc gcagcaaccg aaggtcgggg
     5161 aattgatgcc ctgcgcgagc atctgcttca gttgccggaa cgcgagcacg ccagccaaca
     5221 tagtttccgc ctcgctattg accgcgcatt taccgtaaaa ggtgccgggc tggtcgtcac
     5281 cggtacggcg ttaagcgggg aagtgaaggt aggcgattca ctctggctga ctggtgtaaa
     5341 taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag ccaacagaaa ccgccaatgc
     5401 cgggcagcgt atcgcgctta acatcgcggg tgatgcggaa aaagagcaga ttaaccgtgg
     5461 cgactggctg cttgccgatg tgccgccaga gccgttcaca cgggtgattg tcgagcttca
     5521 aacccataca ccgctgaccc agtggcagcc gctgcatatt caccacgccg ccagccacgt
     5581 cacgggacgc gtttcactgc tggaagataa ccttgccgag ctggtgttcg acacgccgct
     5641 gtggctggcg gataacgatc gcttagtgct gcgcgatatc tcggcccgca acacgctggc
     5701 gggggcgcgt gtggtgatgc ttaacccgcc gcgtcgcggt aaacgtaagc cggaatatct
     5761 gcaatggctg gcgtctcttg cacgggcgca gagcgatgcc gatgcgttat ctgttcatct
     5821 ggaacgcggc gcggttaacc ttgcggattt cgcctgggcg cgccagctca acggcgaagg
     5881 gatgcgcgaa ttgctgcaac agcctggcta tattcaggct ggttatagct tgttgaatgc
     5941 gccggttgcc gctcgctggc aacggaaaat tctcgacaca ttagcgactt atcatgagca
     6001 acatcgcgat gaacctgggc ctgggcgcga acgtctgcga cgtatggcgt tgccaatgga
     6061 agatgaagcg ctagtactgt tgctgattga aaagatgcgc gaaagcggcg acatcctcag
     6121 tcatcacggt tggctgcatc tgccggatca caaagcgggc ttcagcgaag agcagcaggc
     6181 catctggcaa aaagcagagc cgctgtttgg tgacgagccg tggtgggtgc gtgatctggc
     6241 aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg ctacgccagg cggcgcagca
     6301 aggaataatt accgcgatcg tcaaagatcg ttattaccgt aacgatcgga ttgtcgaatt
     6361 tgccaatatg atccgcgatc tcgatcagga gtgtggttca acctgcgcgg cggatttccg
     6421 cgatcgctta ggcgtaggcc gaaagctggc aattcagatt ctggaatatt ttgaccgcat
     6481 tggctttacg cgtcgtcgtg gaaatgatca tt
//
LOCUS       DAZFAH010000223         6462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFAH010000223 DAZFAH010000000
VERSION     DAZFAH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.225"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..390
                     /gene="ydjO"
                     /locus_tag="ACHJWB_004222"
                     /pseudo
     CDS             1..390
                     /gene="ydjO"
                     /locus_tag="ACHJWB_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(394..1785)
                     /gene="tcyP"
                     /locus_tag="ACHJWB_004223"
     CDS             complement(394..1785)
                     /gene="tcyP"
                     /locus_tag="ACHJWB_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4315754.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1918..2508)
                     /gene="ydjM"
                     /locus_tag="ACHJWB_004224"
     CDS             complement(1918..2508)
                     /gene="ydjM"
                     /locus_tag="ACHJWB_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4315755.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLF
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2671..3339)
                     /gene="hxpB"
                     /locus_tag="ACHJWB_004225"
     CDS             complement(2671..3339)
                     /gene="hxpB"
                     /locus_tag="ACHJWB_004225"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4315756.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRHEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3486..4022
                     /gene="yniB"
                     /locus_tag="ACHJWB_004226"
     CDS             3486..4022
                     /gene="yniB"
                     /locus_tag="ACHJWB_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4315757.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4063..4923)
                     /gene="yniA"
                     /locus_tag="ACHJWB_004227"
     CDS             complement(4063..4923)
                     /gene="yniA"
                     /locus_tag="ACHJWB_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO4315758.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAERDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5029..5319)
                     /gene="ghoS"
                     /locus_tag="ACHJWB_004228"
     CDS             complement(5029..5319)
                     /gene="ghoS"
                     /locus_tag="ACHJWB_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4315759.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5420..6349)
                     /gene="pfkB"
                     /locus_tag="ACHJWB_004229"
     CDS             complement(5420..6349)
                     /gene="pfkB"
                     /locus_tag="ACHJWB_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4315760.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMALKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1685 a         1601 c         1575 g         1601 t
ORIGIN      
        1 ggactatata ctatgaaaaa aattgtatac atcctgccgg gagcattttt gctgcgattc
       61 aaaaaaaatg agagtctcct aaagtttcct tcgataatat caatgcttaa tatcaatctt
      121 ctttttaatc cactaaatct tccgggaatg ggaagtggtg tgcttgaaga tattatgagt
      181 attccagata gttcacttag aaaacgtctc gggtatgagg ttttaagctt ctcactacag
      241 gcacattcat tgagccagga atgtattgat aagttggaca tttttttcgc tgacgatctg
      301 tttaaatatg agtcagtatg catcgccgcg atggaacatt taaaaagtaa agcaacggcc
      361 cctatacaaa acggaccgtt gccagcataa gaattaacgg tgtgccagtt cggcgtcgtc
      421 ttcgctatcc agaatggctt tatcggtttg cttcagccac tggctggtca gcgtgccagc
      481 tgtcatcgag ccactaacgt ttagcgccgt acggcccatg tcgataagcg gttcaacgga
      541 gattaacagc gccaccaggg ttactggcag gcccatcgca ggcagcacaa tcagtgcggc
      601 gaaagttgca ccaccaccga caccggcaac gcctgcggaa ctaacggtaa caataccgac
      661 cagcgtcgca atccacatcg ggtccagcgg gttaatgcca accgtaggcg caaccatcac
      721 cgccagcatt gccggataca aaccggcgca gccgttctga ccaatggttg caccgaaaga
      781 ggcggcgaaa ctggcgatgg attcaggaac gcccagacga cgcgtttgtg cttccacatt
      841 cagtgggata gacgcagcac tggaacggct ggtaaaggca aacgtcagca caggccatac
      901 cttacggaag tacttcagcg gactcacgcc attaatgccc agcagaatgc catgcactgc
      961 aaacataatc agcagaccga ggtaggacgc gacaacgaaa cttcccagtt tgatgatgtc
     1021 ttgcaagtta gaacctgcaa ccactttggt cattagagcc agaacgccgt aaggggtcaa
     1081 ctgcatgaca aggcgaacca gtttcatcac ccagctttgt agggtatcga tagcggctaa
     1141 gacgcgttca cctttcggcg catcatcctt cagcagtttc aatgcagcta cgccgaggaa
     1201 tgcggcaaaa attaccacgc tgataattga cgtcggattg gctccggtaa gatcggcaaa
     1261 cgggtttttc gggataaagg acaagaccag ctgcggaacg ctcagatcag agactttacc
     1321 aacatagtta ctttcaatgg cgttcagacg tgcagtttct gcaccaccct gaaccagacc
     1381 ttcagccgtc aaaccaaaca ggttggtgac cagcacaccg accagcgccg caatcagcgt
     1441 ggtaaacaaa agcgtaccga tggtcagaaa actgattttg cctaactgag atgcgttatg
     1501 cagacgggca accgcgctca gaatagaggc gaagactaac ggcataacga tcatttgcag
     1561 cagttgaaca tagccgttac caacgatgtt aaaccactgt acagaatctt taagtacctg
     1621 gctgtcagaa ccataaatgg tatgcagggc aaggccaaaa accacaccca tcaccagacc
     1681 caccagcact tttttcgcca gactccactg tttatggcgg gtctgagcca gcgcaaacag
     1741 cagtacaacg aacaccacga tgttcgcaat taatggaaag ttcatccccg ttctcctgat
     1801 cttattattt ctatcactgc gtagcagtca tagtgttggc gcaagattaa cagaactgtg
     1861 agctggctct tatattcaaa tggaattgct tattccagaa aggtgatttt tgtcttttta
     1921 gtattcaacc tgatgcttaa taagccgatg aaactggatc tgcaaggtat tgatcatttg
     1981 cgatgaccaa cgaacagcgc tgttctcggg taatgaatgg ggcatccata ccgaccagac
     2041 aaataatgcc atgcagataa aacgttccag ttggttgccc ttttggggaa ccaggatagg
     2101 caagcggaaa cgccagcggc atggccagag caggggaaca ccggcgggtg tcaacatatc
     2161 ggcaagtatg tggctcaaat aacccagcac cattccttgt agcgcatcag ccggaatgaa
     2221 ccagccttcc ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg ccagcagact
     2281 gtgggtaaaa ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc attttaatcg
     2341 ctgcccaaga aacgactttg ggtgatcgat gtccgggaac aaacacgtca ggattgcgga
     2401 agggacaata tgccaccagt caccctgtgc cagcacgggc gtcagctcgg catttttggc
     2461 aaataccgca caagcaatag aaaagagaag gtgaccttcc gccgtcatga taaaactcac
     2521 taaactgtcg atacgtacag tataggattt ttatacagtg cacggaagtg gtgacggtgt
     2581 aactctttct cttaaattga cgattaatcc gtcagttgaa tgcaaattca gataaagaac
     2641 caggggcagc gtgatcactg cccctggtca ttaaccgaga aggtcttttg cggtgagttc
     2701 tgtcagcgat gagagtttga cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg
     2761 cgcaggaacg acgatggaac gcatgcgggc tgctttagaa gcgatcatgc catttaccga
     2821 gtcttccagc gccacgcagg tcagtgggtc aacgcccagt tttgctgcgc agtcgagata
     2881 tacttgcgga tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg catcgaaact
     2941 gtcgcgtaag tcaaacatgg tcaacacttt ttccagcata tgtagtggtg acgcggaggc
     3001 cagtcctacc aataaacctt gttctttgca taacgcaacg gcttcgcgca cgcctggtaa
     3061 taatggacgt gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt ctactacttc
     3121 gtgacggctt ggcccattcc acggttgccg ggcgtaccaa agatcgacca ccatatcgat
     3181 gcgtaaacct aaggtgtccg gcagctcgtt acgacgggag atatccaccc ccaggcttgc
     3241 catcacatcc agttcggctc gatcccataa aggttctgag tcgataagta atccatccat
     3301 atcaaaaatt gcagcaagaa tctgacgcgg ggttgacatt acaacctctc ctttagagca
     3361 ttatgtgtaa ggtattaagc cgtgcttatg attaagcgcc tatcatatcg cctttaaaga
     3421 atgggcgaaa acagatatct acaggtaaac taagtgaaac gacgcgtctt aatcaaggag
     3481 aactcatgac gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt ttgggttggg
     3541 tgatttttat acctgcattg atctctacat tgatttcttt gctgaaattt atgaataccc
     3601 gacaggaaaa ccaggaaggc attaatgcgg tcatgctcga ctttactcat gtcatgatcg
     3661 atatgatgca ggcgaatacg ccttttttga atctgttctg gtataactcc ccgacgccca
     3721 attttaatgg tggcgtgaac gttatgttct gggtgatttt catcctgatt tttgtcggac
     3781 tggcgttgca ggattccggt gcccggatga gtcgccaggc gcgcttcttg cgggaaggtg
     3841 ttgaagatca actcattctg gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa
     3901 ttgagtcccg tattgttgtt ccgcaccata ctattttcct gcagtttttc tcactgtata
     3961 tcctgccggt tatttgtatt gctgcaggtt atgtgttctt ttctctgctt gggtttattt
     4021 aatcaaaagg ccgcacagag cggccattat cttcaaccca tatcatgctg ctaataatct
     4081 atccagtgac tgctgagcaa taaccaaatg ctgaccgcca aataaccttg cacgatttaa
     4141 cagcgtgtag agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta gcggggatac
     4201 tgactgatag ccgtcataga tttgtggcgg ctgttcagta tgcagcggta acatcgccag
     4261 gtcgcactct cggtcacccc agtagcaggc cgggtcgaaa atgtacgggc catccggacc
     4321 cagcgcacag ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg gttgatgcga
     4381 ggccagacgc tgctgaatat gctcgacgat ggcgtcgata ttgccgaaag cgatcccttt
     4441 ctctgctgcc agttccaact gccagccaat ccgttgttca gcaaaaaacg ttgaccagcg
     4501 acgttgccag gtgttgggct gtggagttgt ggagagcgcg ttatcgaaat caaggccaaa
     4561 ttgtggttgg tcactccatt gatgtaaacg cgcaatttgc tgaccaagaa taaatgcgct
     4621 atgcgcatcc agcggacgag gtgggagata atccatcacc agaaaactgt agtcacggtc
     4681 agcgccaact gcccaaacct taggtacggt gacggtttta ctacgcgaca gtaactccag
     4741 ttggtcggct tcggcggtaa aaccgggaag cagttccctt tcatcacatt tgacgaaaaa
     4801 gtcacgttct gcatagcgca aatgccatgc ggcatggact tctccgccag gcagttcatt
     4861 acgcagttcg atttcgcctt cacctaactg ctcgctcaaa agacgactga ttgcctgcca
     4921 catgatgtct ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa tacggtgtaa
     4981 aaacacgagc taacgcacac cacagactga ttattgccca aaaaccattt attttctgtt
     5041 gctattccat tcctcccagg tcgtgatggt gatttcagga tctttgccgg ttgcactttg
     5101 ggtaagcccc gaaaccagtt ctctaatttc ttcttcactt tgggtactga taagtccaaa
     5161 agtgttagtt cccagctcat ggatatttcc ctcatcatcc gtcatggtga gcaaaaaacc
     5221 gtcgcgagtc aggtaattat tcaactcgtt aatttcagtc aatgtatcct catgcaacat
     5281 gacggttatg acataacgga caagatcgcc actggccata gttcacccct ttgttatcac
     5341 atgacttttg agcatagtcg gagaaacgcg ttgccgacag gttggtggtg attcccccaa
     5401 tgctggggga atgtttttgt tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat
     5461 gggagcacag acgtgttccc tgattgagtg tggctgcact ccccgcagct acgccaaaac
     5521 ggaccatctc ttcaagagag gcattttctg ccagtttcag tgccatcgcg ccgaccatgc
     5581 tgtcaccagc gccaacggta ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt
     5641 tttcactatc aacacccagc gctccttgtg gaccaaggga aacgacaacc cgtttggcct
     5701 tgccgctatt aacgatttcc tgcgcggctt tgcggacatc gtccggctgg gtgagttcgc
     5761 gattcaccag cgcactgagt tctttttggt taggcttaac caactcgatg ttaccaattg
     5821 ccagtgctgc acttaacgct tcgccagaac tgtcgacgat gcagcggatc ccttgttttt
     5881 gcgcagcgga aatcagttgg gttaattttt ccagcttcac acctggcggc aggcttccgc
     5941 ttatgaccag gatggccccg gattcaattt ccagaacttg ctcttcaagc tggcgaaact
     6001 catcttcatt taatgccgcg cctggcataa caaaacgata ctgctcaccg cttgcttcca
     6061 catgtacatg taaattctgc cgggtccagt ctttggcttc tacagtagcg acggggacat
     6121 tttcatccgc caacagtgaa accaggtgtt cgccggtcgc gccacccgcc gggaagatcg
     6181 ctgtggcact gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg ccgcccccgg
     6241 gttcgaacac cggtgcggta cagcgcagtt ttccttcggg ataaatttgc ggggtaattg
     6301 ttgcgctatc gagagagggc gcaagtgtca acgtatagat acgtaccatc atttcctcct
     6361 ataggctgat ttcagtctgg caccgaatca gcggaaagtg aagaaattaa caatatgatt
     6421 tattggagct ttatttaaaa ttttgcagat aaatatatat aa
//
LOCUS       DAZFAH010000224         6447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFAH010000224 DAZFAH010000000
VERSION     DAZFAH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.226"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(152..1015)
                     /gene="potB"
                     /locus_tag="ACHJWB_004230"
     CDS             complement(152..1015)
                     /gene="potB"
                     /locus_tag="ACHJWB_004230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4315761.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(999..2135)
                     /gene="potA"
                     /locus_tag="ACHJWB_004231"
     CDS             complement(999..2135)
                     /gene="potA"
                     /locus_tag="ACHJWB_004231"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4315762.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2385..3611
                     /gene="pepT"
                     /locus_tag="ACHJWB_004232"
     CDS             2385..3611
                     /gene="pepT"
                     /locus_tag="ACHJWB_004232"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4315763.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3660..4781)
                     /gene="roxA"
                     /locus_tag="ACHJWB_004233"
                     /gene_synonym="ycfD"
     CDS             complement(3660..4781)
                     /gene="roxA"
                     /locus_tag="ACHJWB_004233"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4315764.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5030..6259
                     /locus_tag="ACHJWB_004234"
     CDS             5030..6259
                     /locus_tag="ACHJWB_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4315765.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
BASE COUNT         1702 a         1531 c         1663 g         1551 t
ORIGIN      
        1 ttgttttttt atagcgattt gctaaaaggg agaagaaaaa acgttctcca aaattagatt
       61 gcaactgttt gattttatgg atagaggcat gctgtttact agacagtaaa aagcatgcca
      121 aatttcctat ttaccttttg aatataaagc actaattatc tatttctgtt gccttcttat
      181 tcagcagacg agaagcgcgc cagtaaacca gcaacatcag gcccattacg atagtcagcg
      241 taatgctggt ggctgcacca aacggccagt cacgaatatt gaggaactgg accttgatga
      301 cgttaccgat cagcaggttt ttcgcaccgc ccatcaggtc ggatacatag aacaggccca
      361 tcgctggcag catcaccagc agacatccgg caataattcc cggcatcgtc agtggaataa
      421 tgatacggat aaaagtctgt aacttgctgg caccgagatc gcgcgccgcc tcaagcaacg
      481 gcttatccag tttttcgata ctggagtaca gcggcatcac cataaacggt agcagaatgt
      541 aaaccagacc gataatcacc gcactgggcg tgaacatgat acgaattggt gtgtcgataa
      601 cgcccagcca gagcaaaaac tcgttgagat agcctttggt gctgaggaaa attttcagcc
      661 cgtagatgcg aatcagtgag ttggtccaga agggaacaat cagcagaaac agcagcagcg
      721 gacgcacctt gtgtggtagc ttcgccagaa accaggcaaa cgggtagccc agcaccaggc
      781 aggcgagggt ggcgatcagc gccatattca gcgagtgcaa tagcacttca aaatagagcg
      841 gatcgagcag acgcgtgtag ttatccagcg taaagaccat tttgacgaaa ctggcgtcgt
      901 cgcgggtcaa aaagctggtg ccaatgatca tcaggttggg cagaaagaca aacaacacaa
      961 gccaaccgac aatagtgaca atcactacat tctggaactt acttgtgttc ttcatcagcc
     1021 agtacgacct cccagctttc tacccaatta atggccattt tttggtcgag agagtggtca
     1081 aagtcagggt cgtcttcatt gaagaattcg ctgaccatca ccatcttgcc attttccagt
     1141 tcaacaaccg actccagcgt catgcctttg tagttacgct cacgaacgta accaatcagc
     1201 ccttcagcgt ggttgtcgtc gttaatctct tcaacacgta agtcttccgg gcgcagcaga
     1261 acatgcagtt tttgccccgg ttcaacggcg aagttaacgt agatattaca ttcgcggcct
     1321 tcaacgttgg cgcgtacgcg ctgctcgtct agacgttcga tgacagtggc gttaaacata
     1381 ttgatttcgc caatgaagcc ggcaacaaac aggtttttcg gctcttcgta aatttcgcgc
     1441 ggtgtaccat cttgctcaat acgaccctcg cgcatcacca caatcctgtc tgacatggtg
     1501 agcgcttctt cctgatcgtg agtgacaaag acgaaggtaa taccaagctt acgctgtaac
     1561 gctttcagct cgttctgcat ttgcttacgc agtttgtaat ccagcgctga gagcgactca
     1621 tccagcaaca acagacgagg cttgttaacc accgcgcgag caatggcgac gcgttgctgt
     1681 tgaccaccag agagctgatg cggtttacgt tgagcgaagg tttccagttg caccatccgc
     1741 agggcttcca tcacgcgggg cgtaatttca gcagcggggg ttttttgcat gcgcaaccca
     1801 aaggccacat tttcgaacac ggtcatgtgg gggaaaagtg cgtagctttg gaaaacagtg
     1861 ttcacatagc ggttttccgc cggaacgtgg gtgatgtcct cgttatccag catgatgcgt
     1921 ccggaatcaa cagtttccag acctgcaatc aggcgaagaa cggttgtttt accgcagcca
     1981 gaagggccaa gcagcgtgag gaactcgcca ttgttgatag tcagatccag ctggggaatg
     2041 acctctttac catcaaagca tttgcgaatt cccgccaatt gcaccagcgg tgaaagcgaa
     2101 ctcggttgtt tattcaattt tttactctgt cccatgtaaa cgcaacggat ggcttaccga
     2161 tgcggggttt gtggttaacc accttggtga ctcttaatga gggcggtaat tctacggcaa
     2221 accgcttgaa tcgccaatct ttgttgtgaa ttactggctt agctttatat tcattaaggt
     2281 aatgctgata aatattcccg cttgcagggg taaaagtgac ctgacgcaat atttgtcttt
     2341 tcttgcttct taataatgtt gtcacaaaaa gtgagggtga ctacatggat aaactacttg
     2401 agcgattttt gaactacgtg tctctggata cccaatcaaa agcaggggtg agacaggttc
     2461 ccagcacgga aggccaatgg aagttattgc atctgctgaa agagcagctc gaagagatgg
     2521 ggcttatcaa tgtgacctta agtgagaagg gcactttgat ggcgacgtta ccggctaacg
     2581 tccctggcaa tatcccggcg attggcttta tttctcatgt ggatacctca ccggattgca
     2641 gcggcaaaaa tgtgaatccg caaattgttg aaaactatcg cggtggcgat attgcgctgg
     2701 gtatcggcga tgaagtttta tcaccggtta tgttcccggt gctgcatcag ctactgggtc
     2761 agacgctgat tactaccgat ggtaaaacct tgttaggtgc cgatgacaaa gccggtattg
     2821 cagaaatcat gaccgcgctg gcggtattgc aacagaaaaa cattccgcat ggtgatattc
     2881 gcgtcgcctt taccccggat gaagaagtgg gcaaaggggc gaaacatttt gatgttgacg
     2941 ccttcgatgc ccgctgggct tacactgttg atggtggtgg cgtaggcgaa ctggagtttg
     3001 aaaacttcaa cgccgcgtcg gtcaatatca aaattgtcgg taacaatgtt cacccgggca
     3061 cggcgaaagg agtgatggta aatgcgctgt cgctggcggc acgtattcat gcggaagttc
     3121 cggcggatga aagcccggaa atgacagaag gctatgaagg tttctatcac ctggcgagca
     3181 tgaaaggcac cgttgaacgg gccgatatgc attacatcat ccgtgatttc gaccgtaaac
     3241 agtttgaagc gcgtaaacgt aaaatgatgg agatcgccaa aaaagtgggc aaagggttac
     3301 atcctgattg ctacattgaa ctggtgattg aagacagtta ctacaatatg cgcgagaaag
     3361 tggttgagca tccgcatatt ctcgatatcg cccagcaggc gatgcgtgac tgcgatattg
     3421 aaccggaact gaaaccgatc cgcggtggta ccgacggcgc gcagttgtcg tttatgggat
     3481 taccgtgccc gaacctgttc actggcggtt acaactatca tggtaagcat gagtttgtga
     3541 ctctggaagg tatggaaaaa gcggtgcagg tgatcgtccg tattgcagag ttaacggcgc
     3601 aacggaagta agcgaaaagg gatgcggcat gtgatgccgc atccggctta aatctaaact
     3661 tacccttcga agaaccagta tccgctattg accagcgccg cgagcatcgc gaggaatgac
     3721 ggatcttcca gcgcatcgcc aaaattctcc gcggtcagcg caatgttgct ggcgagtgca
     3781 tccagtgccg gacggtgcgg ggaatcgatc ttctcaccat tggcatacac gtcgtcgcca
     3841 atgcgcaata cgcgcagacc acccaggcgc accagcactt caccttgttt cagcgcatcg
     3901 tagatttcat ctggctgata aggcggttcc ggcggcgcga tatccagttc atgacgtgac
     3961 tgggatataa actcgccaaa ccattgctta aagtgttccg gctggttgat caattcgagc
     4021 atcatctcac gcagtttatc catctcttgc ggcagaacgt ccgcaggatg agcgcgaggt
     4081 ggaacatccg gatcgctgta gtagttgccg cccagttcac gttgcagcac ataatcggca
     4141 aatccgctaa ttaattcccg cgtatttggc gcgcgaaaac ccacggaata gttcatcgca
     4201 ttttccagcg cgtagccttc atgcgggaat cctggcggaa tataaagaat atcgccaggc
     4261 tccagctctt catcgatgat ggcttcgaac ggatcgacct gtaacagatc cgggtgcgga
     4321 cagtgctgtt tcatttgcag cttttcgccc actcgccagc gacgacgacc ggtaccctga
     4381 atgataaaca cgtcgtactg atcgagatgc gggccgacgc cgccgcccgg tacagaaaaa
     4441 gagatcatca gatcatcaat acgccagtcc ggtagttcac ggaacggtcg catcagcgcg
     4501 gcggtcggct catgccagtg gttcactgcc tgtaccagta atgaccagtt ggtttcaccg
     4561 agatgatcgt agctttcgaa cgggccgtgg ctgacctgcc atttgccatc ctggtgactg
     4621 accagtcgac tgtcaacttc gctttccatc gccagacccg ccaactcgtc tggagagatc
     4681 gggtcaataa aattattaaa gccgcgtttt aacaccaccg ggcgtttctg ccagtgacgt
     4741 tcaagaaaat cgggccagtt aagagtgagt tggtattcca tgttaagcat ccacaggctg
     4801 gtatctgcaa ccgattataa cggatgctta acgtaatgcg tgaagtatgg acatatttta
     4861 tcaattagtg ttttttgccg tttcataatg tttcagattg ttcattatgt ttttgattat
     4921 aaacagattt gtgattttcc ttgttccagc ttgtttcacg ttgtagccct ttgtgcaacg
     4981 atcggtgtag ttaatggtgt agttaattgt gatgaccgga atcaaaatta tgagcagagc
     5041 acttaacaaa ctgagcgata cacagctgag gaaaatcaac ggcacacccg cccaaaaaac
     5101 agcctttctt aatgacggtg gaaacctgag cgtcaggcat tcaaccagtg gccttttaac
     5161 ctggtatttc acttacaggg ccggaacggg aaggggggca ccaccggaac gcattaagct
     5221 gggaaattat cctgatctga gcctgaaatc agccagggaa aaagccgccc agtgtcgcgc
     5281 atggctggca gaggggaaaa atccacgtca tgagcttaat tacaccgtac aggaagcgtt
     5341 aaagccggta acggttggcg atgcgctcac ctactggctt gagtcgtacg caaaggaaaa
     5401 ccgcgtggat tatgccgccc tgaaaaagcg ccttaataat cacgtaatac agcacattgg
     5461 tgctatgccg ctggataaat gcgagctacg gcactggctg gcctgttttg accaggtggc
     5521 aaagcgaacg cctgttactg ccggattctt gctacagacg tgcaaacagg cgcttaagtt
     5581 ctgccggagg cggcgctatg caatcagcaa cgttcttgat gatatgagtg tggcggacgt
     5641 tgggaaaaaa ccggatataa gcgagcgtgt cttaagcacc aaagaactgg gcgaattatt
     5701 gcaggcactg gacaaaaaaa tattctcccc ctactacatc gcgttaatcc gcctcctgat
     5761 tgtgttcgga tgccggacgg tagaactgag gttatcggag atcagcgagt gggattttac
     5821 cgaaatgctc tggaccgttc cgaaggagca cagcaaaacg aaggtagcca tattccggcc
     5881 tataccggaa gcaatactgc cgttcatcac gcagctggtg gagcagaaca ggcacacggg
     5941 cttattgctg ggggaagtga aacaggaggc cagcgtatcg cagtatggaa gattagcgca
     6001 caggaggctt aatcaccctc actggtcact gcatgacatc cggcgcacct ttacaaccat
     6061 gctgaacgat ttaggcgttg atccgcatgt cgtggagcag cttacaggcc accagatgcc
     6121 aggaatgcag cgagtttata atcattcccg ttatctggat gcgaaacgca atgcgctgga
     6181 tatgtggacg gagcggttag ggatactggc gggaacacat gaaaacgtaa ccacgctacc
     6241 agtagccaga agaaaataat ttttttcgtg ttttttcagt atgcgcatac tggatatgcg
     6301 aacagataca acgtgcaaca atgagcaaca atgtggaaca actacgaaca agaggcgaaa
     6361 aagcgtacgg atttataagt agctgatttt taatgtgtta ctggtttttt atacactagc
     6421 gaatcactct ttaaagagcg agaaaaa
//
LOCUS       DAZFAH010000225         6442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFAH010000225 DAZFAH010000000
VERSION     DAZFAH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.227"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHJWB_004235"
     CDS             complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHJWB_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4315766.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHJWB_004236"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHJWB_004236"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4315767.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHJWB_004237"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHJWB_004237"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4315768.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHJWB_004238"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHJWB_004238"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4315769.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5270..5389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHJWB_004239"
     CDS             complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHJWB_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4315770.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHJWB_004240"
     CDS             complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHJWB_004240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4315771.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1539 a         1782 c         1566 g         1555 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagccctg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgattgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
     2641 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
     2701 acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
     2821 ggcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcgg caggcaccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cagcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgagcgaga atcaatagct tacgcgctac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgcgccag agtagtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caagaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg
     4141 cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc
     4681 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
     4981 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
     5281 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
     5341 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
     5401 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
     5461 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
     5521 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
     5581 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
     5641 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
     5701 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
     5761 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
     5821 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
     5881 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
     5941 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
     6001 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg
     6061 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gcgtcgggaa aactttaata
     6121 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgcgct
     6181 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
     6241 ccaatatttt ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc
     6301 ttcttgacca aataaccgcg aacagggtga tcactgccaa cttattacgc ggtgcgaatt
     6361 tacaaattct taacgtaagt cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
     6421 tatttcctga ctgtactaac gg
//
LOCUS       DAZFAH010000226         6435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFAH010000226 DAZFAH010000000
VERSION     DAZFAH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.228"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(16..630)
                     /gene="yagU"
                     /locus_tag="ACHJWB_004241"
     CDS             complement(16..630)
                     /gene="yagU"
                     /locus_tag="ACHJWB_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4315772.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            1048..1737
                     /gene="paoA"
                     /locus_tag="ACHJWB_004242"
     CDS             1048..1737
                     /gene="paoA"
                     /locus_tag="ACHJWB_004242"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO4315773.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1734..2693
                     /gene="paoB"
                     /locus_tag="ACHJWB_004243"
     CDS             1734..2693
                     /gene="paoB"
                     /locus_tag="ACHJWB_004243"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO4315774.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSSRALLAGASG
                     QLRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIA
                     THPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPLGKTPHIETALLPGELIVAVT
                     LPPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQL
                     SQGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            2690..4888
                     /gene="paoC"
                     /locus_tag="ACHJWB_004244"
     CDS             2690..4888
                     /gene="paoC"
                     /locus_tag="ACHJWB_004244"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4315775.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEILGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            4898..5854
                     /gene="paoD"
                     /locus_tag="ACHJWB_004245"
     CDS             4898..5854
                     /gene="paoD"
                     /locus_tag="ACHJWB_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO4315776.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            5905..6243
                     /locus_tag="ACHJWB_004246"
                     /pseudo
     CDS             5905..6243
                     /locus_tag="ACHJWB_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
BASE COUNT         1519 a         1783 c         1778 g         1355 t
ORIGIN      
        1 ttatcattca atgcattatc tgtttgagcc taaagggatc tcagggtctg gctcatgagt
       61 aattctgttt cgtaaatctc tgcgaataat ttcaatagac cagaaccaga ctaaatgtcc
      121 aaaaatttca gaaacattct cataccacgg gagatcaaac agaggtggcg tcagtcccat
      181 gagagggaat gaaatcatat gaacaaaaag ttgggctaaa gcacctgcca gtaagccctg
      241 ccagagttta atttttggaa atacttcagc gaccacacaa taaccgacag caaacactat
      301 cgaaaagata atgtgcgtaa caccaaccca gttaaagaca tgcccggcaa aggtataaac
      361 agccgcattg ggatctgtca gccccaacca gtctcgaaga aaaatatacg gtggattgag
      421 aaaattacgc gagcaatcaa tttggcctgc agccctgatt aatgattccg ggccacacgc
      481 tgcattaaac atatccaccg ggctacgtgg cggcaatgga acttcagccc cccacttcac
      541 gaatgcggaa acaacgccag caatcagccc aatgaatgca gcaagaccat aacgtctgcg
      601 gttcggtgga gtttgttcaa atatattcat atctaccctg cttgtaccat tatgttatac
      661 acctcttcag gagtattcat aaaacaaggc aaatgtaaag aactgtattg ttttgtataa
      721 caagatagtt tcctaatcgc caatgaatat aagctccatc atttctccct atttttatat
      781 tagaagtgac agagatttgc agggtgatgc agagctgaaa atcacaggtt tccttattgg
      841 tttttgcatc gtacaactaa agcaataaac cagctcccat catcatcaac atcccggcga
      901 acatgaattt attagccggg ctcatagctg cattcgcgtt ttaagaataa tcctcctgct
      961 gtcgccgact atgcttaacg tttaaaaaag catcagcact ctcgcaacgc actcttattt
     1021 tcccctttag aataccggag gcctggtatg agcaaccaag gcgaataccc cgaagataat
     1081 cgggttggga agcacgagcc gcacgattta agtttgaccc gtcgcgatct gattaaagtg
     1141 agcgccgcaa cagcggcgac cgccgtggtt tatcctcatt ctacgctggc ggcaagcgtt
     1201 ccggcagcta cacccgcgcc agagataatg cccctgacac tgaaggtgaa cggcaaaacc
     1261 gagcagcttg aggtggatac ccgaaccacg ctactggacg ctttgcgtga aaatctgcat
     1321 ttgatcggta ccaagaaagg ttgcgatcac ggacagtgcg gagcctgtac cgtgctggtc
     1381 aatggtcgca ggcttaatgc ctgcctgacg cttgcagtca tgcatcaggg ggccgagatc
     1441 accaccattg aaggcctggg ttcgccagat aatcttcacc ccatgcaggc ggcctttatc
     1501 aagcatgatg gcttccagtg cggctactgc acctccgggc aaatttgctc atcagtagcg
     1561 gtgctaaaag agattcagga cggcattccc agtcacgtca cggtcgattt ggtttccgct
     1621 ccagaaacaa ctgccgatga gatccgtgaa cgtatgagcg gcaacatctg tcgctgtggt
     1681 gcatacgcta acatccttgc cgccattgaa gatgctgcgg gggagataaa atcatgaagg
     1741 cgtttaccta tgaacgagtg aacaccccag ccgaggcggc acttagcgct cagcgcgtac
     1801 ccggcgcaaa atttatcgcg ggcgggacga atctgctgga cctgatgaag ctggaaattg
     1861 aaacgcccac ccaccttatc gatgtgaacg gcctcgggct cgataagatt gaagtgaccg
     1921 acgcgggtgg gctgcgcatc ggcgcactgg tacggaacac cgacctggcg gctcacgagc
     1981 gcgtgcgtcg tgattacgcg gtactctcct cccgcgccct gctcgctggc gcatctggtc
     2041 agttacgtaa tcaggcaacc accgcaggta atctgctcca gcgcacgcgc tgcccctatt
     2101 tttacgacac caatcagccc tgcaataagc gcctgcccgg gagcggctgc gcggcgcttg
     2161 aaggctttag ccgtcagcac gcggtggtag gcgtaagtga agcctgcata gccacccatc
     2221 cgagcgatat ggcggtcgca atgcggttgc tggatgcggt ggtggaaacc atcacgccgg
     2281 agggaaagac tcgcagtatc acactggctg atttttatca cccactgggg aaaacgccgc
     2341 acattgaaac cgccctgctt cccggtgagc ttatcgttgc ggtgacgtta cctccaccgc
     2401 tcggcggaaa acatatctac cgtaaggtgc gcgatcgcgc ctcctacgcc tttgccctgg
     2461 tatcggtcgc ggcgattatt cagcctgacg gcagcgggcg cgtcgcgctg ggcggagtag
     2521 cacataagcc ctggcgcatt gaggctgcgg atgctcagct atcccagggg gcgcaggccg
     2581 tatatgacac gctgttcgcc agcgcccatc ccaccgctga aaacaccttt aaactcctgt
     2641 tggcgaagcg aacgcttgcc tccgtactgg ctgaagcgag ggcacaggca tgaaatttga
     2701 taaacccgca ggggaaaacc cgatcgatca gctgaaggtt gtcggtcgtc cccatgaccg
     2761 catcgacgga ccgctgaaaa ctaccggcac ggcacgctac gcctacgaat ggcatgaaga
     2821 ggcccccaac gccgcctatg gctatatcgt cggttccgcc attgccaaag gacgcctcac
     2881 cgcccttgat acggacgccg cgcaaaaagc gccgggcgta ctggctgtca ttaccgccag
     2941 taacgccggg gcactcggca aaggcgacaa aaacaccgcc aggctgttag gcggccccac
     3001 tattgagcac tatcatcagg ccattgcgct ggtagtggcc gagaccttcg aacaggcgcg
     3061 agcggcggcc tcgctggtgc aggcacacta tcgccgtaat aaaggagctt actccctggc
     3121 ggacgaaaaa caggccgtca atcagccgcc ggaagacacg cccgacaaaa acgtcggtga
     3181 ctttgacggg gctttcacct ccgctgcggt gaagattgat gctacctaca cgaccccgga
     3241 ccagagccat atggcgatgg agccgcatgc ctcgatggcc gtctgggatg gaaataagct
     3301 taccctctgg acctcaaatc agatgattga ctggtgccgc accgatctgg caaaaacgct
     3361 gaaagttccc gtggagaatg tgcgtattat ctccccgtat atcggcggag ggtttggcgg
     3421 caagctgttc ctgagaagcg atgcactgct ggcggcgctc gccgcccgag cggtgaaacg
     3481 tccggttaaa gtgatgctcc cccgccccac tattcccaat aacaccacgc accgccccgc
     3541 cacccttcag cacctgcgta tcggtgccga ccagagcggg aaaatcacag ctatctcaca
     3601 tgaaagctgg tccggaaacc tgcccggcgg cacgccagaa acggcggtac agcaaagcga
     3661 attactctac gccggggcga atcgtcatac cggcctgcgg ctcgccacgc ttgatttgcc
     3721 ggaagggaac gccatgcgtg cgcccggcga agcccccggt ctgatggcgc tcgaaatcgc
     3781 gatcgacgaa ctggcggaaa aagcgggcat cgatcccgtc gagtttcgca tcctgaatga
     3841 cactcaggtt gaccccgccg acccgacgcg ccgcttctct cgccgtcagc ttatcgagtg
     3901 cttgcgcacc ggagcggata aatttggctg gaagcagcgc aacgccacac ccggacaggt
     3961 gcgcgacggg gagtggctag tcggccacgg tgttgcggcg ggctttcgca ataatctgct
     4021 ggaaaaatcg ggggctcggg ttcacctcga accaaacggc accgttaccg tggaaacgga
     4081 catgaccgac attggcaccg gcagctacac cattctggcc cagacggcag cggaaatact
     4141 tggcgtaccg ctggagcagg ttgcggttca cctcggcgat tccagtttcc cggtttctgc
     4201 gggttctggt ggacaatggg gcgcgaatac ctccacctcc ggcgtttacg ccgcctgtat
     4261 gaagcttcgc gaaatgattg cctcggcagt cgggtttgat cctgagcagt cgcagtttgc
     4321 cgacggcaag attaccaacg gtacccgaag cgccacgcta catgaagcca ccgcaggcgg
     4381 cagactgaca gcggaagaga gcattgaatt cggaacactg agcaaagagt accagcagtc
     4441 gacctttgcc gggcattttg tggaggtcgg cgtgcatagc gcgacgggag aagttcgggt
     4501 ccggcgtatg ctcgctgtgt gtgctgcagg acgcatcctg aatccgaaaa ctgcgcgcag
     4561 ccaggtcatt ggcgcaatga ctatgggcat gggtgcggca ctgatggagg agctggcggt
     4621 ggatgaccgt ttgggctact tcgttaatca cgatatggcg gggtatgagg tgccggtcca
     4681 tgcggatatc ccaaaacagg aggtgatttt cctggatgat accgacccca tatcctcccc
     4741 gatgaaggcc aaaggtgtcg gtgagttggg cctgtgcggc gtgagcgcgg ctatcgccaa
     4801 cgcggtgtat aacgccaccg gtattcgggt acgggattat cccatcactc tggataagct
     4861 gctcgataag ctgccggatg tggtttaagg agaaacaatg tcatacccgc tttttgataa
     4921 agacgaacac tggcataagc cagagcaggc gtttctcacc gatgaccacc ggaccattct
     4981 gcgcttcgcc gtagaggcgc taatgtccgg taaaggagcg gtgctggtga cgctggtgga
     5041 gatacacggc ggcgcggcgc gcccgctcgg ggcgcagatg gtggttcgcg aagatggtcg
     5101 ttactgcggt tttgtctctg gcggctgcgt ggaggccgct gccgcttttg aagcgctgga
     5161 gatgatgggc tcaggccgcg atcgcgaaat tcgctatggc gaaggttcgc cgtggtttga
     5221 catcgttctg ccctgcggcg gtgggatcac gctgacgctc cataaactac gctcggcaca
     5281 gcctctgctc gccgtgctga accgtctgga acagagaaaa ccggcggggc tgcgctacga
     5341 tccgcaagca caatcgttgg tgtgcctgcc cacgcaaacc cgaacgggct ggaatctcaa
     5401 tggctttgag gtggggttca ggccatgcgt caggctgatg atttacggac gttctcttga
     5461 ggcgcaggca accgcgagtc ttgcagcagc cacaggctat gacagccata tcttcgatct
     5521 ttttccggcc tcagccagcg ctcagatcga taccgacacg gcggtcattt tgctgtgcca
     5581 tgatctcaac cgggagctgc cagcattgca ggccgcgcga gaagcaaaac ccttttatct
     5641 cggcgcattg ggcagccatc gaacccacac gttacgtctg caaaagctcc acgagctggg
     5701 atggtccagg gaggagacag cgcaaatccg ggcccccgtc gggatattcc ccaaagcccg
     5761 ggatgcgcat actctggcac tctccgtgct ggcagaaatc gcctctgtac gtctccatca
     5821 tgaggaggat tcatgcctgc ccccgtcgtc ctgatccttg cggccgggcg tggagagcgc
     5881 tttctcgcct ccgggggaaa tacccataag tgtatcggct ggcgtcagtc cccggaggtt
     5941 gcgccttatc gctggccatt tgaagaaaac gggagaactt tcgaccttgc gattgaaccg
     6001 cagattacga ctaatgatct gcgtctgatg ttgaggctgg ctcttgccgg cggaggaata
     6061 acaattgcca ctcaggaaac tttcaggcca tatattgaaa gcggtaagct tgtatcgctg
     6121 cttgatgact ttcttccaca atttccgggc ttctatctgt atttcccaca gcgtcgcaat
     6181 attgcaccaa agctccgcgc cctgattgac tacgtcaaag aatggcggca gcaattggtt
     6241 taaatagctg cacctgcact gcctgatgtc agaacagtat tttgatgaat tgccaaggtt
     6301 acaatggcac aaatgcggca caggaggaaa cgtggtgtat ttaaatatgg ggtaactctt
     6361 tgattttaat ggtgcgataa taggaatgaa cattacaagt taacacattg atttataata
     6421 gattataata ttcaa
//
LOCUS       DAZFAH010000227         6327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFAH010000227 DAZFAH010000000
VERSION     DAZFAH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.229"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..1021
                     /locus_tag="ACHJWB_004247"
     CDS             89..1021
                     /locus_tag="ACHJWB_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4315777.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(1063..2151)
                     /locus_tag="ACHJWB_004248"
     CDS             complement(1063..2151)
                     /locus_tag="ACHJWB_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4315778.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(2293..6276)
                     /gene="ehaA"
                     /locus_tag="ACHJWB_004249"
     CDS             complement(2293..6276)
                     /gene="ehaA"
                     /locus_tag="ACHJWB_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4315779.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGGRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVISDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAST
                     SDKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALIGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         1534 a         1703 c         1372 g         1718 t
ORIGIN      
        1 ttattcaaaa atgattatct atctttaacc gatacctcta cagtcatagc tggagaaatt
       61 catagtagat aatggcgaga atggtttaat gaactcaatt tttaccgaag agaatctgtt
      121 ggcatttact accgctgcgc gttttggcag cttcagtaaa gccgcagaag agttgggttt
      181 aaccacttcc gccattagct acaccattaa gcgtatggag acggggctgg atgtggtgct
      241 gttcactcgc agtacccgca gcattgagtt aacggagtcc gggcgttatt ttttccgcaa
      301 agctaccgac ctgctgaatg attttcacgc catcaaacgc agtatcgata ctatttcgca
      361 gggcatagaa gcacgtgtgc gcatttgtat taatcagctg ctttatacgc ccaaacacac
      421 cgcacgcttg ttacaggtgc tgaaaaagca gtttcctacc tgccagatta ccgtcaccac
      481 cgaagtgtat aacggcgtct gggatgcgat tattaataat caggcgaata tcgctatcgg
      541 cgcacccgac acgctgctgg acggtggcgg cattgattac acggagattg gcgcgattcg
      601 ctgggctttt gccatcgccc cggatcaccc gttagccttt gtgccggagc cgatcgccga
      661 aagccagctg cgtctctacc ctaatatcat ggtggaggac accgcacata cgattaataa
      721 aaaagtgggc tggttgctgc atgggcagga gtcaattctg gtgccagatt tcaacaccaa
      781 atgtcagtgc cagattttag gtgaaggaat tggttttttg ccggattaca tggtccgtga
      841 ggcgatgacg caatccctgc tggttacccg acaaatccat aatccgcgcc aggattcgcg
      901 catgttactg gcgactcagc attctgctac gggtcaggtg acgcagtgga ttaaaaaaca
      961 atttgcgcca aatggcatat taacggggat ctatcaggat ttactgcatc gggagaatta
     1021 atcgctgcca acctcccgat gcgctgtgaa tagcggttta catcaaccac ctgctttcat
     1081 tacccattca gagataaatt tattacccgg tactggcgga gagtacaaat agccctgcaa
     1141 aaagtgaacg cctttaccga tcattaaatc cgcctgctcc tgggtttcta ccccttccgc
     1201 cacgatactc agaccaggct tacgcgctag ttcgacaata ttgtccacaa tatgaccgga
     1261 gatttcgtcg acactcgcca tttgcacaaa tgacttatcg atcttaataa aatcgaccgg
     1321 gaacgcctgc aagtaacgat aggtcgcata acccgtacca aagtcatcca gcgcaaaggt
     1381 aatgttgtgc tgatgaaggc tgtcaaatat cgctctggct tctggcgtta ccggaatagg
     1441 gttacgttcc gttagctcga gaaccagctt gattttatcg ttacctaatt tcttaaccag
     1501 gttcagacac tctttttcaa atcccgctgc caaaaaacaa cccgccgaga cgttgatgcc
     1561 aatatggaaa ttgtccggca gcaaatgttt taccggcatc agaatatccg cagtctgttt
     1621 catcagttgg cgggtcatta tgacaataag accggatgac tccgccagag gaataaactg
     1681 atccggtggg ataattcccg tttgtggatg ttcccagcgg acaagtacct cacagcccgt
     1741 cagtacccca gtctgcgcgc agaaaaccgg ttggatccac ggtttgaatt cgtggttttc
     1801 caacgccaga ctgatggctt caggcgtgac gatatggtga caacggttat cagggatata
     1861 aaaattattt ttatttcccg tgccactgat gacttgcgga tgatattgaa aaaagatatc
     1921 ccgccagaag gtaatatcac tctggatgcc tagtttttca tacagctgct tcttttgatg
     1981 tgaaattgtc tttgcactgc gatttctgta ctgtgagatt tctgtaaccg acattccctc
     2041 cattagcagc ttgagtaaaa gccgttcttt ttcggacacg ataccaggca acgtcaaatt
     2101 caccgttgcc ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca ttgaacacac
     2161 cttgattaaa tcttgtgcgc cagctaaatc tgcgagtctt cactctgact gataaagctg
     2221 gctgcaagaa aatgacacag agagccgaat tatctcccgt tcggctctct ctccttttac
     2281 tcattattca gatcagaatt gccacttaat gccaaccatt gcagaggtgt cattatatcc
     2341 cctatcggca acctgaacac ccacattccc ccagacattc aggttggcat ttagctgtcc
     2401 ttccaccccg gtttttatct cagcaatatt tcgggctcca tcctgagtga cggacacgcc
     2461 atccatactg gtgctgaaat ccttactgtt atgcagccag ttcacttcta caaacggctg
     2521 gaactcgcgg gatttaccgt catccatttt atggtggctc ttcagccagg tttttacgcc
     2581 aagtcgggtt tgaacattgc catcaccgtt gctatgaacg agggttccgt tgctttcgcg
     2641 gtgcttatcg gctttgactc ccatccaggt aacctgtgct tgcggctgaa cataccattc
     2701 attacgcgtt ccctggctgc cattaaattc agctaatttg tgtttgtatc cagcttccag
     2761 tgaagcggta aatccttttg atttatagga ttcactttgt aagtcatccc ctttcactgt
     2821 gttatcaaac cagctgtact gcgcccaact gtcgagatac gcgccattac gcgactcgtc
     2881 atcggcatac caggtggcat agaggccggt gctatatccg ttcacactcg cttttgcacg
     2941 ataaccggtt cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg ccatgacccc
     3001 aacatgccag ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc ccagttgcag
     3061 aacatagcga ttgctttggg ttttcagctg gccgctgcca tcacgccatt tattatgacc
     3121 accttcatgg cgcatccaca tagtggtttg tttctgctca cccgtcacca tgtcggtata
     3181 gtacgtatta cccagacgct catgcagacg cgtggtgaac attgtattcg ctgctgcaag
     3241 gttcgcaatg tagctacccg cctccggtcg caggtcatta tccacattca gatccggacc
     3301 cggcgtaggt gtcgggttag ggttcggctc tggattcggg tttggctccg gattcggcat
     3361 cgggtctggc tccggctgca gttcaggaga atcactgccg ctggtcagat accagttacc
     3421 actatttgcc ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa cgatacgacc
     3481 agcctgaaca aattcgccct cagacttacc gtctacatgg ataagttcga taccgttaag
     3541 ggttttcgca cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc cgctagtatc
     3601 gccctcgaca accaatttat cggttactga gtcatcgcca ttcagtaccg tattcattac
     3661 tagttgaccg ttattaccgt ggtagttccc cttcaccact aacacattgc cagcttgctg
     3721 tcctgacatt ccagtcatca gcgtaccgtt gttggtcaga ttgccgccca gggtgtaatt
     3781 cacagacgca gccgctcttt tttcagcgct attattcaac gccagggtac cgttgttctc
     3841 aacgtttgcg ctacccaatc cctgcgcatg acttaccagg acattagccg aattagcaat
     3901 gttcacattc gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc cgccctgaat
     3961 attcagctct ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg taaccgtcag
     4021 actgccgcca tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc ccacggtctg
     4081 gctgtgacca ttcatatcca gttcagtctc tgccgccaga cgcagttcat gggtattacc
     4141 caacacgtta tcgttattca acaaaagcgt cccactgcgc agatcggtaa caccggtata
     4201 gtcgttgtct ttgttagaaa gcgatacggt ctgacccttc tggctgctga atgccaggtc
     4261 accactgccg gtaatctttg cgctgagatc ggcggcattc tcgcttttac cgttagagct
     4321 cagcaccaga gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc cgtaaccgat
     4381 atacagaccg tcattgttta ccccactcgt cagacgatag tcgtaagtgc cgacggccgc
     4441 tttttgccca ttctgctgaa tatcacgttg ggcactgctg gagaggattt gcccattttt
     4501 atcctgcaat tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg catcaaccaa
     4561 cttaatggtg ctgttaccat catcgacttc agtgagcgat aacgcagagt caatatcacg
     4621 agatactgag cttacaacgt cactgtcaga aacctgaatc acaccctcgc cgcgcaaatc
     4681 cagcgtttta ctaacgttga ccgtcccttc tgtcatctgt gcacctgctg tcagggcacc
     4741 aaaatcaacg gttccgccgg agaatcccaa agtgctaatg gagtgagcgc ccttacccac
     4801 tgttaccagg cttccctgac ccgcagtgat cccgcctctc tgcagaaact cagcattctt
     4861 ttccaggtta aatgtcgtgt tctggaacgc cagtgaaccc tggaaccagt ctgtataagc
     4921 aaactcatcg aaggagaatt cgtgattccc catgtttatt tcaacaatac ctctaccttg
     4981 aagatcgtta tcaaataccc aatctttatt ggcattgatt tgcagtttgc catcaacatc
     5041 caggtccgca cggccgatgt tcttgtcttc agaaataacc gcgacagcac cagaagcgac
     5101 atcaatatat ccgctgaatg catagttatt gccgatcagt gccactgtcc cactgtcgat
     5161 acttattgta ccattgccgg aaatcttatt ggcaaaggtc atgctatcgt cagccttcag
     5221 gtgtaatttc ccgcggttgt tgatctctgc tgtacctata ttttgggtga tccctaaagc
     5281 gccattttgc tcaattttga ctttaccact aaaggcagag ttatcaccgg ttaagtaagt
     5341 cgtgccattg agcgcattga tatagcccag gccctcgctg ccactgacaa tatccgcgga
     5401 aatagtcgcg tctttatccg tgtggtttaa ggtgattttc ttatcggatg tcgaagccca
     5461 cacaaactca attttttcgg catcaataat ccctgccgcc ttcgcagcac ttccttcctg
     5521 tgcacctaac gctaactcag agttggtgct taaactaatt ttcggtgcgg atattttacc
     5581 tccatctgat atgactaatg ttgtatcaga acgttggtcg taataaccca ggctaatatg
     5641 ttcacgagca ttaacttcac caccgttatt aaccgacagg aactgattac cgccatatcc
     5701 gccgtaaacg cccataaaga aggtccctgc atttacctga ctaccttcgc cgcttacatt
     5761 cccctgcgta taagtcagac gactggtgcc cttaacatga attttgccgc cattggagac
     5821 attaaaatag ccttgatcat gtctcatgcc tgagtttgac tcatacaaat catcagcata
     5881 taactcgctg ccagcattgg agacattcag gaaccccgta cctttcagca aaaagttaag
     5941 tgggccattg atagttacct ttcctccggt aatatctaac ccaccaccag aagcgagagt
     6001 cagacgcgga cgggaagtat ctgtcgcatt gattaccaga ttggcattgt cagtcaccgt
     6061 aatacggtaa tcgatgctct ggttctcttt gccaaaatca atagttttat cctggtttac
     6121 gatgacatca tcggcgaata ccagagatga atataaactt atcgcgctaa ttaaaacagc
     6181 ccgggacttt tttcctttta ctctgccccc cagctccgag cagacgctcc aacagttacg
     6241 ggacctgttc cactttagcc gatatatttt attcatcaca atctccagca atacatccaa
     6301 ataaaaccaa gattgcataa acagcaa
//
LOCUS       DAZFAH010000228         6308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFAH010000228 DAZFAH010000000
VERSION     DAZFAH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.230"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..298)
                     /locus_tag="ACHJWB_004250"
     CDS             complement(<1..298)
                     /locus_tag="ACHJWB_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO4315780.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHK"
     gene            complement(555..1190)
                     /gene="msrQ"
                     /locus_tag="ACHJWB_004251"
     CDS             complement(555..1190)
                     /gene="msrQ"
                     /locus_tag="ACHJWB_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4315781.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1191..2195)
                     /gene="msrP"
                     /locus_tag="ACHJWB_004252"
     CDS             complement(1191..2195)
                     /gene="msrP"
                     /locus_tag="ACHJWB_004252"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4315782.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISATALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPYVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2304..2717)
                     /gene="hiuH"
                     /locus_tag="ACHJWB_004253"
     CDS             complement(2304..2717)
                     /gene="hiuH"
                     /locus_tag="ACHJWB_004253"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4315783.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVNAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2850..3521
                     /gene="hprR"
                     /locus_tag="ACHJWB_004254"
     CDS             2850..3521
                     /gene="hprR"
                     /locus_tag="ACHJWB_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4315784.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISCLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3521..4879
                     /gene="hprS"
                     /locus_tag="ACHJWB_004255"
     CDS             3521..4879
                     /gene="hprS"
                     /locus_tag="ACHJWB_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4315785.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4977..5828)
                     /gene="hchA"
                     /locus_tag="ACHJWB_004256"
     CDS             complement(4977..5828)
                     /gene="hchA"
                     /locus_tag="ACHJWB_004256"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4315786.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
BASE COUNT         1738 a         1327 c         1497 g         1746 t
ORIGIN      
        1 ctttgtgata ataatcttta atttcagcaa atgttttagt tttatctgca tccgctttct
       61 tctgaaagac ggggtcaagt ttgccacttt gcagtaaagg ataaacggat tgccagactc
      121 catcccagtc actgagcgtt cggttttgta cattggcatc atcaaaaacc ccattagcag
      181 ctttttgttc gacctctgtt aagggtttgc cgtgtgagtg atgaccatgc gaaaaggcag
      241 gagcgctaac aataaagaca cctaaagcaa cagccagttt gtaaagacga atcgccaaaa
      301 cagtttcctc cagagttaat gtatatgatg tgtaatgtta tattgtaaca tatagcaaaa
      361 tgcaacaact cttaggaaac gagagcatgg ctttctcagt tctacttatc tatcttattg
      421 aaataaaata aattattttg gtgatttgag gttgatgaca aacgaaacat aacctgatgc
      481 gatctgtgta tgccggatag ggcgctcgcg ccacatccgg cagggataac gcgtactttg
      541 tcggcaatat gaagttatac agataatttg ttgtgaacct gcttgcgtaa ccggttaaaa
      601 agggaacgca acttcttata ccgtaaggct aaaagcagta cagccagccc agcgtagatg
      661 agcggctgcg gtgaaataat cttcactgac cacagataat gtattggggc cagaatcgcg
      721 acaagataga cgaaattatg caactgttgc cagtgcttgc cgagttttcg ctgcatcgcc
      781 tgggttgaag tgaacgctaa agcaagcaaa attacccagc tgataatacc taacgttaaa
      841 taaggccggg taattaactc cttacctaat aacgccagat tattcacgcc cagctccagc
      901 aatgcgtaac ttgttaaatg cagtgtcgcc caggcaaagc accataatcc taacaggcgg
      961 cgagtgcgta tcaataacgg ctgttttgcg tagcgtgcca gaggtgtgat taacaaggtc
     1021 gccagcaaaa atttcagtgc agtgcgacca gtaaaatgct gaatatcttt caccggatcg
     1081 gcacccagtc caccgtgatt gatcgcccag accagccaga gaaacggcaa caatccggca
     1141 agatgcaggc aaactttcag ccatgtcacc tgttttgctg tcagacgcat ttagaaattc
     1201 tcccgcaaat ccaggccacg atacagcgat gccacctggt cggcgtaacc attaaacagt
     1261 agcgttggct ggcgctgtac atcgaggatg ccgcctgaac caataaatcg ttcggtagcc
     1321 tgtgaccagc gcgggtgatc aacatacgga ttaacgttgg cgtaaaaacc gtattcgtca
     1381 ggcgctgcca gattccaggt ggttggcgga cgctcgcggg tcagcttaat actgactatc
     1441 gatttaatcc ctttaaagcc atatttccac ggcacaatca gtcgcaccgg cgcgccattt
     1501 tgtggcggta acgccttgcc ataaacacct acggtcatca gtgtgagcgg atgcattgct
     1561 tcgtcgagac gcaatccttc gacataagga tatttcagcc caccgccgat aaagcggtcc
     1621 tgctggcctg gcatctgttc cggtgcataa attgtttcga aagcgacata cttcgcattg
     1681 ctggtaggtt cggcaagcgc cagcaatttg tgcagcggaa aaccaatcca cggcaccacc
     1741 atcgaccacg cttccacgca gcgcatacga taaatacgct cttccagcgg gaagcgacgg
     1801 gttaagtcat catgatcgag ggtcaatggt tttgccactt cgccgctgat tttcagtgtc
     1861 catggatcgg ttttcaggct accagcatta gcggcgggat cggctttatc cagcccgaat
     1921 tcatagaagt tgttataacc ggaaacttta tctgctggcg tcagtggcag gttattttgc
     1981 caggcggcag gcttgctgaa ctccagcgct tttccggcgg gggcgggcgg gcgatcgttc
     2041 cctttaaacc agctaagcag atcggcatgc gcagcgtgag gcaaagaaag tgcagttgca
     2101 ctgatgccca gtgcttttaa cacctgccga cgcttcataa agaataccga ctcggccgta
     2161 acatctgatt cttttaaaaa ttgattcttt ttcataagcc atcacacttt tttgtcgtta
     2221 gccagcaaga gccgtttact gttaatctgt cagggagtgt cctccctaac agtagcagag
     2281 atctgctttt acatatttat gcattaactg ccacgatagg ttgaataccc atattggcta
     2341 agtaataaag gcacatgata atgctcgttc actttattaa tatgaaattc aacggggatc
     2401 tcagggaaga aactttcaag attttgtttc ttgaaatagt ccccggtttt aaatacgaca
     2461 cggtaatcgc ccgtagttgc agtttgctcg ggccacagtg ccttaattcg tccatcctta
     2521 tctgttttgg cggtattaag ttgtaaccag ccgttatccg ccttcttttc aagagtgact
     2581 gtcacgtcag cagcgggttt tcctgtttgc tggttcaaaa tgtgcacgct aagaatgttt
     2641 tgttgtgcgg cattaaccaa agaaggtaat gaaaatgctg ccgttgctac cgacagtact
     2701 aaataacgct ttaacatgtt tatatccttg tcatgtgaat gagttttgag gatagcgaaa
     2761 aagcgtccag ggtaacctta cagcagcatt acaattttgt aatgaaccag gctgtttcta
     2821 taacatatga tttatggcat attattttca tgaagattct acttattgaa gataatcaaa
     2881 ggacccagga atgggtaacg caggggcttt ccgaagcggg ttatgtcatc gatgccgttt
     2941 ctgatggcag agatgggctt tatcttgcgc tgaaggatga ttatgcattg atcattctgg
     3001 atattatgct tccgggtatg gatggctggc agatcttaca aacgttaaga acagcaaagc
     3061 aaacccctgt tatttgcctt actgcaaggg attctgtcga tgacagagtc agagggctgg
     3121 acagtggggc aaatgattat ctggtaaaac ctttttcatt ttctgagttg ctggcaaggg
     3181 ttcgggcaca attaaggcaa catcacgctt tgaattcaac attagaaatc agctgcttaa
     3241 gaatggactc tgttagtcaa agtgtgagca gggacaatat cagtattaca ctgacgcgca
     3301 aggagtttca gttactttgg ctactggcct ccagagctgg cgaaattata cccagaacgg
     3361 ttattgcgag tgaaatttgg ggaatcaact ttgatagtga taccaatacg gtggacgtcg
     3421 ccattcgcag gctccgcgca aaagttgatg atccttttcc tgaaaagcta atcgccacaa
     3481 tccgggggat gggctattca ttcgtagcgg taaaaaaata atgaaaagat tatctataac
     3541 cgtccgttta accttgcttt ttatattgct gctgtctgtt gctggcgctg gaattgtctg
     3601 gactctctat aatggcctgg caagtgagtt gaaatggcgc gatgatacaa cactcattaa
     3661 ccggacagca cagatcaagc agttgttaat tgatggggta aatccagata cgttacctgt
     3721 gtactttaac cggatgatgg atgttagtca ggatatcttg atcattcatg gtgatagcat
     3781 caataaaatt gttaaccgga caaatgtcag tgatggcatg ttaaataaca tacctgctag
     3841 tgagacaatc agcgcagctg gcatttacag aagcattatt aatgacacag agatagatgc
     3901 tttacgaatt aatattgatg aagtttcgcc atcattaacg gttactgtgg ctaaattggc
     3961 ttcagccaga cataacatgc ttgaacagta taaaatcaat agcattataa tttgcattgt
     4021 cgccattata ctgtgctcag tattaagtcc gctgttaatc cgaacggggt tacgagagat
     4081 caaaaagttg agtggtgtaa cggaagcgct gaattataac gatagccggg agcctgttga
     4141 ggttagtgca ttaccgagag aactaaaacc tcttgggcag gcgttgaata aaatgcatca
     4201 tgctttagtc aaagattttg agcgtctaag tcagtttgct gacgatctcg ctcatgaact
     4261 tagaacgcca attaatgcat tactgggtca gaatcaggtt acgctcagtc aaaccagaag
     4321 tatcgctgaa tatcaaaaaa caattgccgg aaacattgaa gagctggaaa atatttcgcg
     4381 gttaacagag aacatactgt ttcttgccag ggcagataaa aacaatgttt tggtgaaact
     4441 ggactcgctt tctctcaata aggaagtcga aaatttgttg gactatcttg aatatctttc
     4501 agacgagaaa gagatttgct ttaatgtcga gtgcaatcag caaatctttg cggataaaat
     4561 tttactgcaa cgaatgttat cgaatcttat tgttaacgct attcgatatt cgccagaaaa
     4621 atcgcgtatt catataacca gttttcttga taccaacagc tatcttaata ttgatatcgc
     4681 cagccctgga acgaaaatta atgagcctga aaaactcttc cgtagatttt ggcggggaga
     4741 taattcgcgt cattccgtag gtcagggact tggtctttct ttagtcaaag cgattgccga
     4801 attacatggg ggaagtgcta cgtatcacta tctcaataag cataatgtgt tccggattac
     4861 gttaccgcaa agaaattaac atagggcttc agtacgcccg tgaaaaaacg cagccacgtc
     4921 gtgagtacta acgcggctgc gattgattat gcgcttacat tcaaacgtaa cagggattaa
     4981 cccgcgtaag ctgccagcat ttcctgcgcc gccagtttac ccaacgcatt cgctgcaaaa
     5041 ggactgtcgc cggtgagaag tttacggtcc ttatgtactc gcccggtgat gtcgtcatta
     5101 atgatgttca tgcccatttt cttcagttct tcgccgaagt accaggtgag atgacccggc
     5161 atatagccaa tctctggcgt ttgtttgtct gcggcgtctg ggaatgcgca aatggaataa
     5221 ccattcagtg ggttatcgcc gtggcgaagc gccagaaaag ccgccgggcc gtggcaaagg
     5281 gagataacaa aacggtcatt tttgattgcc cactgtaaag cggcagctac gtcctggctt
     5341 tcaggtaaac caataagtgc gccatgacca ccaggaacaa agattgctgc atattcgcta
     5401 tcagcgttga ggctggcgac aacatccgcg agtttcttcg gattgcggaa caacgattta
     5461 tgctgctcaa agaatggcat tactttttca tctttgtgcg gcatagccca gtattcaaac
     5521 ttggtcatca gaccggaaat ggtcgccact tcgaattcga aacctgcagc atggagatga
     5581 tacaacggca gcaacgtttc aatcggatgg ttaccggtcg agaacagttt tccgttatcg
     5641 gtcggcaaat aacgttcgtc cgcggcgatc accagaattt tatgtttacc gcgataaggt
     5701 tttggatagt ccacgccatc aagatcagag acaggactgg tatattggct aagcgaatat
     5761 tctgaaggga agaatgcatt atcttcagca atatcgacct gcggattttt acttgtttga
     5821 acagtcatag tgtattcctt attgttgctt agttagggta gtcactatat ttgcgactga
     5881 gctggtcgct atttttaacc aggctggaaa attgagtcaa tatatcgaaa gcggtagtcg
     5941 gcgggtgtaa gtatgtaatg atgatgctgc actggtgttg aatattattc acgccaaatt
     6001 ttccttattg agcaacgtcg tcgcaagtgg cctcaatgat tttagaccat tatcagaggt
     6061 acagggcggc ggggagagat ttagtgctgt ggaatattgt ggtgctgatg aatatttata
     6121 tcccggattg gtagagaaaa agctttttcc atgcactttt tttgcaaata tttctatatg
     6181 ggaagtgtgt ttgttgcgga ttaattcata aagaaatgca tttggggtgt tggcttgaag
     6241 agtcgctggt tgatgttatt gtgccaggag gggcagggta atgtgccgta acgatatgag
     6301 gtgtactg
//
LOCUS       DAZFAH010000229         6294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFAH010000229 DAZFAH010000000
VERSION     DAZFAH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.231"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..769
                     /gene="yjbM"
                     /locus_tag="ACHJWB_004257"
                     /pseudo
     CDS             1..769
                     /gene="yjbM"
                     /locus_tag="ACHJWB_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            1132..1236
                     /locus_tag="ACHJWB_004258"
                     /pseudo
     CDS             1132..1236
                     /locus_tag="ACHJWB_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            1377..1958
                     /locus_tag="ACHJWB_004259"
     CDS             1377..1958
                     /locus_tag="ACHJWB_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFO4315787.1"
                     /translation="MRLHLPELDDVVWMKGVLMLNVRFDSEKALEAILYVASKAPIPD
                     IYHVGKILYYADRFHLESFGRLITGDHYNAMKDGPVASNTYDIIKIARGNGRYIPNGC
                     DVDSVRKAFSVSGMTIVPSREADEDFFSDSDLECIDKSIAMLGNMSFEAIRTMSHDAA
                     WEQADHNGEMSLESIVAQLKNSKLILDYLKNGY"
     gene            1964..2425
                     /locus_tag="ACHJWB_004260"
     CDS             1964..2425
                     /locus_tag="ACHJWB_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315788.1"
                     /translation="MLGDCFPPDFKANFSRERGISPGDVLYLHCDFTTPPKVKYMVVV
                     CCEPLLVLLINSDINEFIKRNNDLMACQVEINREDHDFLKWDSFVNCIEAHAAFDLEI
                     IKEKIAIQYGDVLKGRITDHCMRQVRCAVEISKTMVKRHKKLILAALQHYE"
     gene            complement(2459..2731)
                     /locus_tag="ACHJWB_004261"
     CDS             complement(2459..2731)
                     /locus_tag="ACHJWB_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFO4315789.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRAEWERVRTHKQRLI"
     gene            complement(2768..3340)
                     /locus_tag="ACHJWB_004262"
     CDS             complement(2768..3340)
                     /locus_tag="ACHJWB_004262"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO4315790.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYTVVSL
                     ESAMGQGATPDGDTILWWLKQSSEARAAICIDDTLSISDALSELSHFINQHADNTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            complement(3340..3651)
                     /locus_tag="ACHJWB_004263"
     CDS             complement(3340..3651)
                     /locus_tag="ACHJWB_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315791.1"
                     /translation="MSNYLYWSGLVANIALMLFVALCIWVWFIWPFVEAMSITRCFIC
                     ASKTSGCKPTVRAIIRTLKYWYLDLLFGRGWTRISNRQFEWEGVGNWRIHSSKETQEV
                     K"
     gene            complement(3675..3932)
                     /locus_tag="ACHJWB_004264"
                     /pseudo
     CDS             complement(3675..3932)
                     /locus_tag="ACHJWB_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
     gene            complement(3906..4031)
                     /locus_tag="ACHJWB_004265"
                     /pseudo
     CDS             complement(3906..4031)
                     /locus_tag="ACHJWB_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(4033..4224)
                     /locus_tag="ACHJWB_004266"
     CDS             complement(4033..4224)
                     /locus_tag="ACHJWB_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315792.1"
                     /translation="MIGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDLPVDIKLRNVEIKVK"
     gene            complement(4226..4690)
                     /locus_tag="ACHJWB_004267"
                     /pseudo
     CDS             complement(4226..4690)
                     /locus_tag="ACHJWB_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4667..4813)
                     /locus_tag="ACHJWB_004268"
                     /pseudo
     CDS             complement(4667..4813)
                     /locus_tag="ACHJWB_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4804..5340)
                     /gene="yfdR"
                     /locus_tag="ACHJWB_004269"
     CDS             complement(4804..5340)
                     /gene="yfdR"
                     /locus_tag="ACHJWB_004269"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4315793.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSGLRKCA"
     gene            complement(5468..6292)
                     /gene="yfdQ"
                     /locus_tag="ACHJWB_004270"
     CDS             complement(5468..6292)
                     /gene="yfdQ"
                     /locus_tag="ACHJWB_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO4315794.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         1733 a         1340 c         1350 g         1871 t
ORIGIN      
        1 ataatttatg gcatgtacgt aaaaaaatgt gtttttaatt gagcaaataa atgatctcaa
       61 aatgtgggtc aataagtata ttgacgattg cactgatgag gatttaaacg atcgtgactt
      121 tattgcatca gttgttgacc gggctatttt tcatttcgcg attaatagta tatgtaatcc
      181 tggggataat aaagatgcga ctcccattga acaatgtact tttgatgtag aaactaagaa
      241 tggccttccc tccacggttc agctatttta tgaggaatct aaggataatg aacctttagc
      301 gaatatacat tttcaagcaa taggttctgg ttttttaacg tttgttaatg cctgccagga
      361 acatgatgac aacagcttaa aattatttgc tttgctgtta atttcacttt catattctag
      421 tgcctacgca gatttatcag aaacagtgta tattaatgaa aataatgaga gctacctgaa
      481 agctcagttt gaaaaattat ctcaacgtga tatgaagaag tacctgggag agatgaagcg
      541 tctggctgat gggggagaaa tgaattttga tggctatctg gataagatgt cacatctggt
      601 gaatgaagga acgctcgatc ctgatatttt aagcaaaatg cgagatgctg caccacaatt
      661 aattagcttc gcgaagtcgt ttgacccaac ctcaaaggaa gagattaaaa tacttacaga
      721 cacttctaaa ttaatttatg atttgttcgg ggttaaatcg gagaaataat atgtgaagtt
      781 cttcgatagt atggaaggca ttatataaaa ggacccaata tttattgggt tcttttttct
      841 ctatcaatgc tattagcagg gagatatatc accagagttt aatgtgtgat tttttattta
      901 tcgtcgaacc tggattgttt atcattggcc ttaacaaagt taacggctaa taagattatt
      961 tccatcactt cgtgagagct tcatgccttg agaggatctc aattttcttt tgcaatgaga
     1021 caggcgcttc ctgttgttat ggtatagtac cccgctattg agcctcctga acagtgaagc
     1081 tgaataacat aaccccatga tatattgata aaataatctc tacatttgaa aatgcacggt
     1141 aattctgaaa tgcaaaaaat caaccaaacc agcgcaatgc ctgaaaaaac tgacgttcac
     1201 tggagtggtc ggtttagcgt tgcaccaatg ctcgatagga tgtaccgttc ttgaaaaaca
     1261 ggtggttata cactttgtgg gggcctattg ggaacctgtt cttcttagaa agcatcattt
     1321 gctcatcact tcaattgacc tagcgtaggt gaagacttta agattgcaga aggcggatgc
     1381 ggttgcattt gccagagctt gacgatgttg tttggatgaa gggtgtattg atgctaaatg
     1441 taaggtttga ttcagaaaag gctttagagg ccattctgta cgtcgcctct aaagcaccga
     1501 tcccagatat ctatcatgtc ggaaaaattc tttattatgc tgacaggttt caccttgaaa
     1561 gttttggacg cctcataacg ggcgatcact ataacgctat gaaggatggg cctgtagcaa
     1621 gcaacacata cgatattata aaaattgctc gtggaaatgg acgttacatt cctaatggtt
     1681 gtgatgtcga ttctgtgcga aaagcatttt ctgtatcagg tatgacgatt gtgccgagcc
     1741 gtgaagctga tgaagacttt tttagtgact cagatttaga gtgtattgat aagtctatcg
     1801 cgatgcttgg aaacatgtcc tttgaagcta tcagaaccat gagccatgac gcggcatggg
     1861 aacaggcaga tcacaatggt gagatgtctt tggaatcgat agttgcacag ctaaaaaata
     1921 gcaaattaat actggattat ctgaagaacg gatattgata cttatgctgg gtgattgttt
     1981 ccctcctgat tttaaggcta attttagtcg tgaaagaggt atatcacccg gtgatgtatt
     2041 gtatcttcat tgtgatttta cgaccccacc taaagtaaaa tatatggtgg ttgtttgctg
     2101 tgaacctctt ttagttcttc taataaattc agatataaat gaatttatta agagaaacaa
     2161 tgatcttatg gcttgtcagg tcgaaattaa tagagaggac catgattttc ttaaatggga
     2221 ctcatttgtt aattgcattg aggctcatgc tgctttcgat cttgaaatta ttaaagagaa
     2281 aatagccatc caatatggtg acgtgctaaa aggtcgcatt acagatcatt gtatgagaca
     2341 agttcgatgt gcagttgaga tatctaaaac tatggttaaa cggcataaaa aactgatact
     2401 cgctgctctt caacattatg aataaaccca tctttggatg ggtttattca tattctattt
     2461 aaatgagcct ttgtttatgg gttctgaccc tttcccactc agcacgccct tcttctcgcc
     2521 ttttgtctat gtattccgca agatcctgaa tattgatgca acgttttgct ttttgtgatg
     2581 tgccgatgcg atatgttgga acgggcaact tacaagcgtt tgcttttgct tctgccgtgg
     2641 ctggactcat gccaaagtac ttttggctaa ctgctgagag ttcaatgtta ggggtattga
     2701 attcagccat cagtaaaaac aaggtgttca taattttctc catcaaaacc ggctgcaccc
     2761 gggaaaatca taattctgtg ctggtggcag gaattagttt ctgccagata gcggaaacat
     2821 attttgcctg atgacgggca tcagccaggg cgttgtgccg ttcgccatcg aaaggcatgt
     2881 ccatttttgg gtcgaatccg atggaacgcc caagcgtaac gatcgtgcgt acatcgtggt
     2941 cattccagta cgcccacggg cagatttgtc ctgctcgctc gtaagctcca cgtaaaatta
     3001 cgttgtcgaa ggtggccccg ttaccccaga cttttaaata ttttgtattg tctgcatgct
     3061 gattaatgaa atggctcagt tcagagagtg catcgctgat cgacaaagta tcatcaatac
     3121 agattgcagc tcgtgcttca gagctctgtt tcaaccacca caggatggta tcgccgtcag
     3181 gtgtagctcc ttgccccata gcactttcca ggctaacaac cgtatagaat tcttgtccga
     3241 tgtctccggt ttctggagtg aagaacaccg cgccaatgga aacgatcggt gcatccttat
     3301 ttttccccat cgtctcaagg tcgatcatta agttattcat tacttcacct cctgcgtttc
     3361 tttgctgctg tgaattcgcc agttaccgac gccttcccat tcaaactggc ggttactaat
     3421 tcgcgtccag cccctgccga aaagcaaatc cagataccag tattttaaag ttctgataat
     3481 tgctcttaca gttggtttgc atccagaagt ctttgatgcg caaataaagc accgagttat
     3541 gctcatggct tctacaaaag gccagataaa ccaaacccag atgcaaagag ccacgaacaa
     3601 catgagcgcg atgttagcca ctaagccaga ccagtacaga taattgctca ttggttgcct
     3661 ccaccttggc tctgaagcat ggcagcgcgg caggcgttcc agccatcaac atacccagcc
     3721 atgatatatc gattaaaacc cacctctttc tgtgcgcgtg ccagattcat ttcctccggc
     3781 acgtgtactg gctgagctat atatagcggc tgaacatacc aaccctttga taaccaactg
     3841 tcagcaacgt ttttactccg tgttattgcc ggaataccta agccattgtc tgaatgcagc
     3901 catgccaccg gttctgcttc cagcgatgcc agcgtaatcc gtgcaagttc catttgttca
     3961 ccacgggtaa gcccgttttc aagcggattt ttaatgaata attcgatacg ttctttggta
     4021 atagaattca tgctatttca ccttaatctc aacatttcgc agctttatgt ctactggtag
     4081 gtctgacttt cctgttaacg ctaatgcgag attttcagga gtaatgagag cagttattgt
     4141 tttccccatt gccagacgaa taatcattcg tatctcgcga tcgtcacatg ctcccggtcg
     4201 aacaattgat atttgtccga tcatctcact ctcctttgat gcgaatgcca gcaagccagt
     4261 ttcttatgcc gatatattca gcgttcctga aaccgctttt tacatatata aatggcaagc
     4321 gaagattgtg accgttggct gccaggtagt ctttacaacc ctgttcggtg aaacagcagg
     4381 taacgaattc atcaatatct ttcacagcaa cgcgccgcca tttttctggt ggttcccgaa
     4441 agttttcatg aagtagttcg agacgacgac tttggagttt attggcttca ttgccatctt
     4501 catcaaccca gacaatccgg tcatagtcat aatcagcatc aacaacaatt tcgcgctttt
     4561 gatacacaca aaacataggg tctgacgtta ttcgattatc ctgtgttcga atattttcac
     4621 cgatgatgcc aaacgaatct ggcgtaggtt ttgtctgtaa ctcttcgata cgttcagcca
     4681 gcgccgcgca cttggcctca gcttcagcaa atttacgcac caggtactca gcgtttgttt
     4741 cgttcacttt cagatctcgc ggtacacatt tcccgcgaag aaacccttcc atttcgaaaa
     4801 cattcatgcg catttgcgta accccgataa ctcgttaaaa cgttccataa acatcccgta
     4861 ggcatggcct ggtgacagtg gaataacttt gaacatctct gtcgccggga taccttccag
     4921 tacaggccag aaagagccat catcaagccc gagatcgcgg cgttcggttg ccagcataat
     4981 gagatcggca tatttcacag gcgtgctcat aaccgggggt aacccgtatt tctcacggat
     5041 tacggcgtct attttttctt ccatccgttt atagtcagga agaaggcgtt tcagtggcgc
     5101 ggggatgtcc tggcaatacg cttctgttgc atcatgcatt aacgcttcaa aagcaaattc
     5161 ctgtggcacc agctggctgc aaagcaccgc atgttgggcg acgctgtaga agtgtgaaag
     5221 atgtcctgca aagcgacaga tatttgaaag ggaaaccgcg atatcgttaa tcacgatgtc
     5281 gtctttattt atcctgtcat aataaaaatg cttcccggaa aaagttttaa taaatgacat
     5341 tttgttctcc acgtaaatgt gttgcaccac gctgaattct ggtaaaagga agccctcacc
     5401 atccggtgat tattgagtta attacgtttc cataaatgcc cccgcagggg catttgcagt
     5461 aatgaaatca ggcggtgaaa gtaccaataa aggtttctac tttgctgtct ttgaatttct
     5521 caacaagcag atcacgaaat tcgttagcca tttcctcctg cactgcttcc agctgaataa
     5581 tgcgcagaac cagtacagga cgatcgccag tgataatgct gaggcgtaat ttaaacggac
     5641 gttctttcag gccttcaaac ggaacgcatt tgaattcaaa tgccactggc ataatgtctt
     5701 tggttttcgc ttcgacagac tccatcaggg agcgtttgcc gctgaagtca ttgtcttcaa
     5761 aatcagcggt ctggtttgct tcaatcgtga ttttacggac tgccgcagct gcttttgttg
     5821 cctgaatagc gtcaccatta gcatcaaagc ccacaaggta gtccgcccag tcttcaatcc
     5881 attctgccag tgacttctgg gagttacgct cgccattaac agacaacaga gcagagaacg
     5941 gtgctgtctt tttcagtttg agagtggcgg tgttatctgc gtggcctggt tcatcaatag
     6001 taccaagatt aagcacactg acagcacgca tattatcagc atcgataaag cagcgggtgc
     6061 cttcatctgc aagatcttta gaataacggg taaagtcatc gatgctggca gtggaaagcg
     6121 caccacggaa acggaagcga tttaaattaa atttttccag atcatgaatg cggaaattct
     6181 caggcaatgc cacagcatca gcaccaatct tactgataat ttcattaaca ccctgagcag
     6241 aaataagggc atggatttga ttaattgcag ttgcgtctaa gttctgagac ataa
//
LOCUS       DAZFAH010000230         6255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFAH010000230 DAZFAH010000000
VERSION     DAZFAH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.232"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..70)
                     /locus_tag="ACHJWB_004271"
     CDS             complement(<1..70)
                     /locus_tag="ACHJWB_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315795.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIR"
     gene            complement(283..1953)
                     /gene="betA"
                     /locus_tag="ACHJWB_004272"
     CDS             complement(283..1953)
                     /gene="betA"
                     /locus_tag="ACHJWB_004272"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4315796.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRANTAHGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1967..3439)
                     /gene="betB"
                     /locus_tag="ACHJWB_004273"
     CDS             complement(1967..3439)
                     /gene="betB"
                     /locus_tag="ACHJWB_004273"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4315797.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3453..4040)
                     /gene="betI"
                     /locus_tag="ACHJWB_004274"
     CDS             complement(3453..4040)
                     /gene="betI"
                     /locus_tag="ACHJWB_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4315798.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4169..6202
                     /gene="betT"
                     /locus_tag="ACHJWB_004275"
                     /pseudo
     CDS             4169..6202
                     /gene="betT"
                     /locus_tag="ACHJWB_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1285 a         1728 c         1729 g         1513 t
ORIGIN      
        1 cacgaatctt tttcttcaga taaaataaaa tctcaatcag atcgatatcc tgtttgattt
       61 gttcacgcat aatatatcca gaaaataaaa tctgtcgcag ataaggttgt attaatagtc
      121 tgtatcagga atgttcgggt taaatatcag caaaaagcca gcatcatgaa tactggatat
      181 gaagcatgag agttacctca gtgtttatat aaggattcgg tccccctctc tggaacggta
      241 actctcaatc tgatcggttc ctgcgttagt tcacatcacg actcattttt tcgctctcac
      301 cggcatccca tttgccacaa aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg
      361 aatcatatcc gctattttct cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat
      421 aatctgcggc ataatcgacg catccaccac acgcaggcct tctaacccgt gtacgcggcc
      481 ttcgccgtca accacggaca tctcgtcgta acccattttg caggtaccgc acggatggaa
      541 ggcggtttcg gcgtggttac gcacgaactc atcgagctgt tcatccgtct gacattccgt
      601 gccggggctg atttcgcggc cacgatactg atccagcgcg ggttgatgca tgatctcgcg
      661 ggtgatgcga attgcgtcgc ggaactcctg ccagtcctgc tcgtgcgaca tgtagttaaa
      721 cagaatcgcc ggatgctggt gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg
      781 cgagcgcatt gagccgacgt ggcactggaa accgtgctcc ttcaccgcat tcgagccgtt
      841 atagttaatc gctactggca ggaaatggta ctgaatattc ggccacgcaa attcctcacg
      901 gctgcgaata aatccacctg cttcaaagtg gttgctggca ccaacgccag tgccgccaaa
      961 cagccactcc gcaccgattt tcggctggtt ccaccactgc agggcagggt agagggaaac
     1021 cggttctttg cactcatatt gtagatacat ctccagatga tcctgaagat tttcgccgac
     1081 gccgggtaat tcatgcacca gcggaatatc aaactccgcc agcagttcag cgttgccgac
     1141 gccggagcgt tgcaggatct gcggtgaggc aatcgcgcct gcacataaca gcacttcttt
     1201 gttggccgtt gcgcgggttg ggatggtgct gtcgccttcc agccattcga cgcccaccgc
     1261 gcgtttgccg tcaaaaatga tgtgatcggt catagcgtga gtacgaatgg tcaggttagg
     1321 gcgcgatttg gcctgatcga gataaccgtg cgcggtattg gcgcgacggc cctgcggcgt
     1381 gacggtgcga tccatcggac caaaaccttc ctgctgataa ccgttgagat cgtccgtgcg
     1441 cgggtagccc gcctgcacgc ccgcttcaat catcgcttca aacagcggat tgacgccggg
     1501 tttggaggta gtgacgctca ccgggccatc gccaccgtgg tagtcgttct cgcccacatc
     1561 gcgagtctcg gccttgcggt agtagggcag gcagtcgaga taactccagt tctccagacc
     1621 gggttcttgc gcccagttat cgagatccag cgcattgcca cggatgtagc acatgccgtt
     1681 gatcagcgac gatccaccca ggcctttacc gcgtccgcac tccatgcggc ggttattcat
     1741 aaacggttca ggctctgttt cataggccca gttgtagcgt ttgccctgta gcgggaacgc
     1801 cagcgctgcg ggcatctggg tgcggaagtc aaagcgatag tccgggccgc ccgcttcaag
     1861 cagcagcacg gaggtattcg gatcttcagt cagacgggta gcgagaacgt tgccggctga
     1921 gccggcacca ataatgatgt agtcaaattg caaataaacc tcctggttag aatatggact
     1981 ggaatttagc catctcaacc tggatggact tcacctgggt gtaactctgg agcgtcatca
     2041 cgccgttctc gcgaccaatg ccggagtgtt tgtagccgcc aacgggcatc tctgccgggg
     2101 attcgcccca ggtgttgatc cagcaaatac ccgcttccag ctgatgaatg acgcgatgcg
     2161 cgcggttcag gtccgccgtc acgatacccg ccgccaggcc gtagtcggta tcattggcgc
     2221 ggcgaatcac ttcgtcctca gattcataag tgagaagcga catcaccgga ccgaagatct
     2281 cttcgcgcac gatggtcatt tcgtcgcggc aatcggtgaa cacggtcggt gcaacccatg
     2341 cgccgttatc gaagccatcg cctttcagta catcgccgcc gcacagtacg cgcgcgcctt
     2401 cctctttgcc tttggcgata tagcgcagca cgttatcgcg atgcgggaag ctgaccagcg
     2461 ggccgaagtt agtttgcgga tcgaaaacgt cgcccgcgcg aatgcgctca acgcgcgcca
     2521 gaattttctg ctcaaatgcg gctttgcatt tcgccggaac gaagacgcgg gtgccattgg
     2581 tacacacctg accggagctg aagaagtttg ccatcatggc gatatcggcg gcgagatcga
     2641 gatccgcatc atcgaaaacg atcagcggtg atttaccgcc cagttccatg gtcacttctt
     2701 tcagggaaga ggccgccgag ttagccatca cttttttgcc gctggcgaca ccgccggtaa
     2761 atgacacttt ggcaatgccc ggatgctcgg tcagatattg cccggtctcc gcgcccaccc
     2821 ccggcaacac gttaaatacg ccgtccggca ggcccgcttc gctgtaaatt tcagccagct
     2881 ttaacgcggt aagcggggtg acttcgctcg gtttgaaaat cattgcgttg cctgccgcca
     2941 gcgccggggc ggatttccac agggcaatct gaatcgggta gttccatgca ccaatccccg
     3001 ccaccacacc cagcggttcg cggcgggtat aaacaaatga cgtctcacgc aacgggatct
     3061 ggctgccttc cagcgccggg atcagcccgg cgtagtactc cagcacgtcc gcaccggtaa
     3121 cgatatcgac ggttgaggtt tccgaatatg cttttccggt atcgagggtt tccagttttg
     3181 cgagttcgtc attgcgttca cgcagaatat caacggcccg acgcagaata cgcgagcgct
     3241 ccatagcggt catcgccgcc cagatttttt gcccctgttg ggcactcttc acggcgcgat
     3301 cgacatcctc acgcccggcg gcctgcacgg tcgccagcac gttaccgttg gccgggttaa
     3361 tggtctcgaa ggtgcgaccg ctggtggcgg aggtataacc accatgtata taaagctgct
     3421 gttctgccat tcgggacatc acgtctcctc ggttaatcgg tgggtaaatg ctgagtaata
     3481 aagtgacggg tcagcgagtg tgcgagaggt ttatccagcg gtttgccgct tagtgccgcg
     3541 cgcagccata atccgtcaat cagcgcggca aggccgtagc ccgcttcctg tgcctgttcg
     3601 cgcggcaatt cgcggcgaaa ctcgctcacc agattcgaca gcaagcggcg gctgctgacc
     3661 tgctgtaaac gatagagcat cggctgatgc atactgctgg cccagaacgc cagccaggct
     3721 ttcatcgccg cactgctcac ctgagtttca tcgaagtttc cggcaacaat cgcctgtaat
     3781 cgctgctctg cactgccctg cggaagtgca tgtaatcgat tcaaaaccgc gtcacgtaac
     3841 tgactggtga tgtcgcgcat ggttgcttcc agcagaccat ttttgtccct gaaatagtgg
     3901 ctgatgatcc ccgtagaaac gcctgcacgg cgggcgatct gcgcgatcgt tgcatcgtgc
     3961 atgcccactt catttattgc ttccagtgtg gcgtcgatca gttgtctgcg ccggatcgac
     4021 tgcatcccca atttgggcat tttcgccact ccattcatca gcattgttta tatattaaag
     4081 cggttattga ttggacgttc aatataaaat gtgtcttaat tgttacgaat ttgattttaa
     4141 atggtaacaa taacagtggg gatactggat gacagacctt tcacacagca gggaaaagga
     4201 caaaatcaat ccggtggtgt tttacacctc cgccggactg attttgttgt tttccctgac
     4261 aacgatcctg tttcgcgact tctcggccct gtggattggc cgcacgctgg actgggtgtc
     4321 taaaaccttc ggttggtact atctgctggc ggcaacgctc tatattgtct ttgtggtctg
     4381 tatcgcttgt tcgcgttttg gttcggtgaa gctcgggcca gaacaatcca aaccggaatt
     4441 cagcctgctg agttgggcgg cgatgctgtt tgctgccggg atcggtatcg acctgatgtt
     4501 cttctccgta gccgaaccgg taacgcagta tatgcagccg ccggaaggcg cgggacagac
     4561 gattgaggcc gcgcgtcagg cgatggtctg gacgctgttt cactacggct taaccggctg
     4621 gtcgatgtat gcgctgatgg gcatggcgct cggatacttt agctatcgtt ataatttgcc
     4681 gctcaccatc cgctcggcgc tgtacccgat cttcggtaaa cggattaacg gacctatagg
     4741 tcactcagtg gatattgcag cggtgatcgg caccatcttc ggtattgcca ctacgctcgg
     4801 tatcggtgtg gtgcagctta actatggctt gagcgtactg tttgatattc ctgattcaat
     4861 ggcggcgaaa gcggcactga tcgccttgtc ggtgatcatc gcaacgatct ctgtcacctc
     4921 cggtgtcgat aagggcattc gtgtgttatc ggaactcaac gttgcgctgg cgctggggct
     4981 gatcctgttc gtgctgttta tgggcgacac ctcgttcctg cttaatgcgc tggtgctgaa
     5041 cgttggcgac tatgtgaatc gctttatggg aatgacgctc aacagttttg ccttcgaccg
     5101 tccggttgag tggatgaata actggacgct cttcttctgg gcatggtggg tggcatagtc
     5161 gccgtttgtc ggcctgttcc tggcgcgtat ctcgcgtggg cgtaccattc gccagttcgt
     5221 gctgggcacg ttgattattc cgtttacctt cacgctgtta tggctctcgg tattcggcaa
     5281 tagcgcgctg tatgaaatca tccacggcgg cgcggctttt gccgaggaag cgatggtcca
     5341 tccggagcgc ggcttctaca gcctgctggc gcagtatccg gcgtttacct ttagcgcctc
     5401 cgtcgccacc attactggcc tgctgtttta tgtgacctcg gcggactccg gcgcgctggt
     5461 gctggggaat ttcacctcgc agcttaaaga tatcaacagc gacgcccccg gctggctgcg
     5521 cgtcttctgg tcggtggcga ttggtctgct gacgcttggc atgctgatga ccaacgggat
     5581 atccgcgctg caaaacacca cggtaatcat ggggctgccg ttcagctttg tgatcttctt
     5641 cgtgatggcg gggttgtata aatctctgaa ggtagaagat taccgccgtg aaagtgccaa
     5701 ccgtgatacc gcgccgcgac cgctggggct tcaggatcgc ctgagctgga aaaaacgtct
     5761 ctcgcgcctg atgaattatc cgggcacgcg ttacactaaa cagatgatgg agacggtctg
     5821 ttacccggca gtggaagaag tggcgcagga gctgcggttg cgcggcgcgt acgtggagct
     5881 aaaaagcctg ccgccggaag agggacaaca gttggggcat ctggatttgt tggtgcatat
     5941 gggcgaagag caaaactttg tctatcagat ttggccgcag caatattcgg tgccgggctt
     6001 tacctaccgc gcacgtagcg gtaaatcgac ctactaccgg ctggaaactt tcctgttaga
     6061 aggcagctag ggcaacgacc tgatggacta cagcaaagag caggtgatca ccgatattct
     6121 cgatcagtac gagcggcacc ataactttat ccatctccat cgtgaagcgc cgggccatag
     6181 cgtgatgttc ccggacgcgt gattgttatt gcataaaacc gcgccatgtc tgcatatggc
     6241 gcgggaatca ttgac
//
LOCUS       DAZFAH010000231         6191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFAH010000231 DAZFAH010000000
VERSION     DAZFAH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.233"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..246
                     /locus_tag="ACHJWB_004276"
                     /pseudo
     CDS             1..246
                     /locus_tag="ACHJWB_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            296..535
                     /locus_tag="ACHJWB_004277"
                     /pseudo
     CDS             296..535
                     /locus_tag="ACHJWB_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(583..1200)
                     /gene="ureG"
                     /locus_tag="ACHJWB_004278"
     CDS             complement(583..1200)
                     /gene="ureG"
                     /locus_tag="ACHJWB_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO4315799.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1212..1886)
                     /locus_tag="ACHJWB_004279"
     CDS             complement(1212..1886)
                     /locus_tag="ACHJWB_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO4315800.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1887..2351)
                     /gene="ureE"
                     /locus_tag="ACHJWB_004280"
     CDS             complement(1887..2351)
                     /gene="ureE"
                     /locus_tag="ACHJWB_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO4315801.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2361..4031)
                     /gene="ureC"
                     /locus_tag="ACHJWB_004281"
     CDS             complement(2361..4031)
                     /gene="ureC"
                     /locus_tag="ACHJWB_004281"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO4315802.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(4057..4377)
                     /locus_tag="ACHJWB_004282"
     CDS             complement(4057..4377)
                     /locus_tag="ACHJWB_004282"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO4315803.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4386..4688)
                     /locus_tag="ACHJWB_004283"
     CDS             complement(4386..4688)
                     /locus_tag="ACHJWB_004283"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO4315804.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4698..5477)
                     /locus_tag="ACHJWB_004284"
     CDS             complement(4698..5477)
                     /locus_tag="ACHJWB_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO4315805.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5858..6133
                     /locus_tag="ACHJWB_004285"
     CDS             5858..6133
                     /locus_tag="ACHJWB_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315806.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1412 a         1598 c         1658 g         1523 t
ORIGIN      
        1 caacgttact ctaaagagtt caaagccgaa gctgtcagaa cggttcttga aaatcaactt
       61 tcgatcagtg aaggcgcttc ccgattatct cttcctgaag gcactttagg acaatgggtt
      121 accgccgcca gaaaagggct cggtactcct ggttcccgca cggtggctga actggaatct
      181 gaaattctgc aactgcgtaa ggcgttaaat gaagctcgcc ttgagcgaga tatattaaaa
      241 aaagcagctg tagattcaat ctgtcaatgc aacacccctt tcaattatct ctttcactgc
      301 ccgcgacggt atcggttatc agtttgacct gctggctaac ggtgaaaccg tactgcgtga
      361 tgcatccggc gaaaaacgca tcaagggtga aaaagacggt cgtatgctca tgaatgttgg
      421 cctgaatgcg gaaattcgcg acaacatccg cttcggtctg gagtttgaaa aatcggcatc
      481 tggtaagtac aacgtggata acgcggtgaa cgccaacttc cgttattcct tctgataaaa
      541 acaaggggcc gcctgccggc cctgatacag aggatgttgt gattacttac ccagcatgcc
      601 ctgctcttcg ataaaggcga tgatgttttg cagaccttca ccacttttca ggttagtgaa
      661 cccccatggg cgttctccgc gcatacgtaa agtatcacgc tccattacct cgagggaggc
      721 gcccacgtat ggtgcaaggt cagttttatt gatcacgagg aagtcggatt tggtgatccc
      781 cggaccgcct ttacgcggga tcttttctcc ttctgccaca tctatgacat atatggtaag
      841 gtccgccagt tcggggctga aggtcgcact caggttgtcg ccgccgcttt ccacaaagat
      901 cagatcgaga ttaccaaatt tctcactcag tgcttccacg gctgccagat tcattgatgc
      961 gtcttcacga atggctgtat gagggcagcc ccccgtttcg acaccgacaa tgcgttccgc
     1021 ttccagtgct ccggcctcag tgagaatgcg ttgatcttct ctggtgtaga tgtcgttggt
     1081 gactacggct aattgccagg tgtcacgcat ggatttgcag agggcttcca gaagcgcggt
     1141 tttacctgag ccgaccggac cgccgacgcc gacacgcagc ggttgcttga ttatgttcat
     1201 tttattttct cctaagaacg gaataatcgg gaatactggg tttcatgtcg cgcggaagcg
     1261 atagatgcaa gtggcgttgc tgaaccaatt gcgtgatccg gggttgcaag ggcactgtcc
     1321 atgttcgccg catagcgggc acacaggcgc ataattaact gctgggcagc cagctggccg
     1381 tagggtacca gcctgacgcc cgccatcact gcgctttcaa tccagctgta gccaaggctc
     1441 agggccaggt ctgaaacgga tatttgccag cgtacgccga gccatgccat accggttaac
     1501 tggctttgct ggcatagttt gcgccattcg gccgggcagt cgggctgcca gtcgaccaga
     1561 agtctggtga aggctgcacc gcggttacgt tcttcatcgc gtagctcacg ggtttccctg
     1621 caggccagca gataagcggt ccagcgcccg gcactgtcag gatcgcccgc ttcacaggcc
     1681 cggtatagcc gggcaagcag tggcaggtcg acggcaaaaa aactctgttc catctgcaac
     1741 tgctgccagt gttcaaaggc ggcgctatct tctacccagc ctgcttccac tgcccactcc
     1801 agtccctggg accagctgta gcctccaaca gggaggctgt tactggcaag ctgcatcagc
     1861 cgcagacgtt tttccggagt cggcattcag tggctgtgct cctgatcgtg attatgggcg
     1921 tgcgacttgc tggcgtaagc gccagcttcc ggctcaaacg gaagatgtgc aaaatcaaca
     1981 tccagcccaa actggcgcag catatcatcg aggacgtgat cgtgatggta gcgcagctct
     2041 ccgggcatta tttgcagcgg aacgtggcga ttgccgagat gccagcaggc tttcgcgagc
     2101 ataaaaggat ctgcgcagcg cactacagaa acggcttcat cagcggcgat caccttaatg
     2161 aattcatcac cgttttcgtt actgagaata tcaccatcgc gcagcagcag accgcgtggc
     2221 agcaacagtc cagcctgacg tccatcgtta agggtgactt taatacggct ttttacccgc
     2281 atgtcaacag ggagggtaac gctggcagtt gtctgagccg gggtctccac ccgccgggtc
     2341 aaataaagca tttgtgctcc ttaaaacagg aaataacgtt gcgccattgg cagaatgtcc
     2401 gccggttcac tggttatcag ttcgccgttg atacgcactt cgtaggtttg tgaatccacg
     2461 gttatatcag gcaacaggct gttgtggcgc atatcggctt tttgtactgt gcggcagcct
     2521 tttaccacag cagttgtgct gtgcaggtta agctgttcag cgacgccatt tgctgctgct
     2581 gcctgcgaca ggaaagtcac acgacagcgg tgacgggcac tgcccaatgc agcgaacatt
     2641 gggcgatagt gcaccggctg cggtgtgggg atagagccgt tgatatcacc catcggcgcc
     2701 atggctatca ttccgccttt gacgatagtc gccggtttta cgccaaagaa cgccggggac
     2761 cagagcacca gatccgccag ttttcccact tcaatcgagc cgacttcatg agcaataccg
     2821 tgggttaatg ccggattaat ggtgtattta gcgatatagc gcttcacgcg gacgttgtcg
     2881 ttatcaccac tttcttccgg taacgggccg cgctgaactt tcatccggtg tgccacctgc
     2941 caggttcgta acactacttc tccgacgcgt cccatggcct gcgaatcgga cgaggtgagg
     3001 gagaacgcgc caaggtcgtg caggacgtct tccgcggcaa tggtttcctg gcgaatgcgc
     3061 gattcggcaa aggctacgtc ctcggcgata tccgggtcaa gatgatggca aaccatcagc
     3121 atgtccagat gctcatcaat agtgttgacg gtatagggta gcgtcggatt ggttgaggaa
     3181 ggcagaatat tggggtgcgc gcaggcggtg ataatatccg gagcatgacc accacctgca
     3241 ccttctgtat ggaaggtatg gatagtgcgc ccgccgatgg cagtcagagt atcctcaacg
     3301 aatcctgatt cgttaagcgt atcactgtgt agcgcaacct gaacgtccat ttcgtcggct
     3361 acagtcagtg cgcagttgat taccgcaggt gtagctcccc agtcttcgtg aattttgagg
     3421 ccgataaccc cggccgcgac ctgctcacga agcgcatccg gattggagca attgccttta
     3481 cccagcaacc cgatattgac cggcaggctg tctgcagcct gcagcatttg ataaatgtac
     3541 catggtcctg gggtacaggt tgtggcgtta gaacccgctg tcgggccagt accgccaccg
     3601 atcatggtgg taatgccgga tgtcagcgct tcttcagcct gctgtgggca gatccagtga
     3661 atatgcgtat cgacgccacc tgcggtaacg atcctgcctt ctgcggcaat aatttccgtg
     3721 gatacgccga ttgggatagt gacgttgggt tgtatatcag gattaccggc tttgccgata
     3781 gcaaatatcc ttccatcttt gacgccgata tcggctttaa cgatccccca gtaatcgatg
     3841 atcagggcat tggtcagcac cagatcagcg cagccggcgg agagcatttg cccctgtccc
     3901 ataccgtcgc ggattacttt accgccgccg aatttgacct cttcgccgta ggtagttaaa
     3961 tcatcttcga cctcgatcca cagctcagtg tctgccagac gaattttatc accggtggta
     4021 gggccgaaca tgtcagcata ggcctagcgt gaaatattac tcatcatttg cctccagctc
     4081 acccattata tctccgcgga aaccgtgcac gatacgggca ccgctcagcg ccaccagatc
     4141 aacctcacgc ttttggcctg gttcgaagcg cactgcagtt ccggcggcga tattgagacg
     4201 atagcctctt gccttctggc gatcgaactt cagtgccggg ttaacctccg cgaagtgata
     4261 atgggaaccg acctgaattg gtcggtctcc gtgattttca acgataatac tgcaggtagc
     4321 ccggccgatg ttaagctcta tatagccagg tttaacctta tattctccag ggatcatctg
     4381 ttacctcaga ttatcggatt atggacggta accagcttgg agccgtccgg aaaggtggct
     4441 tcgacctgga tatcggggat catttctggt atgccttcca tgacctgctc gcgactcagg
     4501 acatgccgtc cttcttccat cagcgcagcg acgcttttgc cgtcgcgagc gccctccatt
     4561 ataaaagcgc taatcagggc cacggattcg ggatagttaa gtttcaggcc gcgggccaga
     4621 cgacgctctg ccagcagtgc agcggtaaac agtaatagtt tgtctttttc tcttggagtc
     4681 agttccatag cgttctctta agtcagccag atccggggaa gtaccggtga tttgtcagtc
     4741 agttgcggcc gcataaattg ccagatatca cgcattacct gctggcaacg cagattatca
     4801 tcacttaaaa agcgaactgt cagtagacca tcggtgagcg tggctccggc gtagttttcc
     4861 agtggtgtga gtttttcgcg tatgtcttcc agttgcgttt cattccccag gtagaacagc
     4921 atcgttccca cccatggttt ccgggcaacg caggtaagtt ctccattggc cacgtgcaga
     4981 cgctcaatca acagcggaga accgtcgacc catatctcca gacggttagc gagtgttccg
     5041 tggctgaagg tttctccgat gaccggacgt ccaaggcaca gaagatccca ggccagcagt
     5101 gtactggagg acgacagatg aaacacggta tgcaacgtgg cgtgagcgcc gggaaaaaaa
     5161 atcgaatcct gtggcaacca ctccagcgtt gcgttattgc acagcgtcag gttttgctga
     5221 aggtgcgcct gcgcgccgct gctgcggtaa aatttactgg caccaggcat ggtgatcagc
     5281 gtatggcaat cagagtcaat tattgcgttg atgatgagtt cgtcaccgcc gacgatacca
     5341 ccgggtggat gaagcagaca gaggtggcag gtttcttctt cgggataaaa aggacgctga
     5401 acagtgagcg gaccgacatg atgacgggtg gtgagcgtgg ttttcccgtg cagacgctga
     5461 aaacggagat ccagcatggc ctgccagcct tttttatttt gtggtaacgc ggtactgagc
     5521 aatcttacgc ccctgataaa taaagataaa acgacagtaa agagccggat ttccttgtta
     5581 tataaaagat gatgcctttc cagtgagttt tcgtctgaat aatacgaatg tttgttttcg
     5641 tcatgaagcg ttccggctcc cggggctata catcgccaga aaagcaatgc atattgtcgg
     5701 gattatccag agaatcaggc agaaagatcc gacaatgggc tgtgattatg ccatctggct
     5761 cagaatgata tcaaggtgtt atggggataa cttgctgata caatagcagt atgacagatt
     5821 cattaatgcg gaaactgctc actgaaacag agtgttcatg actgagcact ggtcgggtca
     5881 tgcagatgat aatttcccct tttcttgggg aagcgacagt attttcatgc atgcctgtct
     5941 tcagtcatac ggggagagca tctgtgatac agtccataca tttgttgcgg acgtcgctcc
     6001 tgagtcactg actggtctgc aaacagttta tctgtacacg gcagggcttc tgtgtgatat
     6061 cgtttttcat atcgaggggg ggcctccaga gatgaatgga ataataatat ttcctgatga
     6121 tataatacct tagagaattt ctgtgtatgc tattgtgaac atatttttta ttaaaaagat
     6181 gttagtggtg t
//
LOCUS       DAZFAH010000232         6105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFAH010000232 DAZFAH010000000
VERSION     DAZFAH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.234"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            365..1849
                     /locus_tag="ACHJWB_004286"
     CDS             365..1849
                     /locus_tag="ACHJWB_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4315807.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2171..2773)
                     /locus_tag="ACHJWB_004287"
     CDS             complement(2171..2773)
                     /locus_tag="ACHJWB_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315808.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2860..3066)
                     /locus_tag="ACHJWB_004288"
     CDS             complement(2860..3066)
                     /locus_tag="ACHJWB_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4315809.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3818..3967
                     /locus_tag="ACHJWB_004289"
     CDS             3818..3967
                     /locus_tag="ACHJWB_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO4315810.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(4312..4509)
                     /locus_tag="ACHJWB_004290"
     CDS             complement(4312..4509)
                     /locus_tag="ACHJWB_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315811.1"
                     /translation="MVIRKTVCRDCDNAIPHNTECCPGYVSADPFDYYRNTDRLLCLL
                     TLLPVLILAVVSGVSVFVLLQ"
     gene            4713..5282
                     /locus_tag="ACHJWB_004291"
     CDS             4713..5282
                     /locus_tag="ACHJWB_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4315812.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHAALVHFPAWPAVWLERDDDAGLR
                     DMLKRADYLAKEYTKVNGTGERNFGCSRS"
     gene            5542..5943
                     /locus_tag="ACHJWB_004292"
     CDS             5542..5943
                     /locus_tag="ACHJWB_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4315813.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            5931..>6105
                     /locus_tag="ACHJWB_004293"
     CDS             5931..>6105
                     /locus_tag="ACHJWB_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4315814.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQM"
BASE COUNT         1556 a         1421 c         1607 g         1521 t
ORIGIN      
        1 gcattcagtg caggaactgt agtcttcttt attttattct tcttcaggag ggggggattc
       61 ctgaaggcgt tattcccggt gttctgttta tgattcttcc cggcaggaaa ccggtatctg
      121 caatacccgg gatgatgctt tttgttcgta cgtggttgtg taaaatttga atagtggatg
      181 tgctctgaag ggaaggaggg gacacacaga ttcattttgt ctttcttcag cagcaaaaca
      241 cactgtggtt attatttaac cattaaatca acaaaaattt ttacctcata ttgatttgac
      301 tgctattttt gctattaata gccagaattg cagtgctttt atatctgtgt gtagtgaggt
      361 tactatgtca ttcggtcagc cttgtgatga gtttcccctg agttcgctgc ctccactcat
      421 tcgtgatgcc gttattgaag ctcagcaaat tacgcaggct ccccttgggc ttgtggcagc
      481 gtctgccctg ggcgctgttt cacttgtctg ccagaatctg attgatgtct gcagactgaa
      541 tacactgcgg gggccggtct ctcttttttt cctgacgctt gccgagtccg gagaacgaaa
      601 aaccgcagtg gacaaattat taatgaagcc cctgtaccag caggagatgc agttatattc
      661 cagatacaaa agtgaactgg ctgtctggaa aaacaaggaa gagcttttaa aggctcagaa
      721 aaaagcattg ttgtcaaaac tgaataaaga actccgcaaa ggcgcggatg agtcagaaac
      781 cctcagacaa ctggaagtac ttcagaaaaa cagtgcggaa gaacctgtga ggtataaatt
      841 catttttaat gatgccacga ctgcggcaat taagaatcag ctttgtggta aatggcgttc
      901 agtgggtatt atgtcagatg aagccggtat tatatttgat ggatataccc tgtctgagtt
      961 gccttttatt aacaaaatgt gggatggctc tgtattgtct gtggacagaa agaatgaacc
     1021 cgaacagatg attgaaaatg ccagaatgac gttatccctc atggtgcaac ccggtctttt
     1081 tgaccgatat atggagcgaa aaggttctgt tgccagggac agtggttttc ttgcccgttg
     1141 tcttatcagt aaacctgcaa ccacacaggg gaaacgcttt attaacggag ctgtgattcc
     1201 gggaggatca ttaacggcat ttcatgaacg attaatggaa ctggccagag gcagtattga
     1261 aaaatccagt gaggatgaac ggtattgtct tcatttttcg cctgaagcgc aaaaaatatt
     1321 tattgagcac tataatgttc tggagcagga tttatcccct tccggaccct taagcccgtt
     1381 tcggggacat gtttcaaaaa aaacagaaaa cattgcaaga attgcagcgt tatttcagta
     1441 tttcagctat ggtgaaggga aaatctctgc cgatatcatg acgtctgctg tcgtaataag
     1501 ttcgtggtac acagatgaat ataaaaaatt atttgcgtta cctgatgaat ctgaactgca
     1561 gcagaaggac gccgaggagc tgttcgactg gctcattgag gagtgccgcg gagaatgccc
     1621 gccccgtgtc aggaaaaatt atattctcca gtgtggcccg ggccggttca gaaacaggaa
     1681 aaaactgaac gcattgctga acattcttga atctcagttc aggctttctg tcgtgccgga
     1741 aggaaaaacc atgtatgttc tgcttcccca gatagccagt ttaaagttgt ctgatgtctc
     1801 tggtattttt acctcgggtt atcactacaa taagctgaga gcgaagtaac cggtgagcag
     1861 tttttctcca ttatgcttca gacttagatg attgattatt attgggattt catcaccgat
     1921 attcagatgt atgaatatcg gtgatgaact aaagctcagg agctgcgtgg tggtgatgaa
     1981 tcaacaggca tataactgat acccgccggt cagaagttcc agcctgcgac tttcctgtct
     2041 gcgtgcccgc agtccggcgt tcaggtgcag cgtgtgacag aacgcacgga gagcgaaacg
     2101 gcaggcagac aggccgttca gtcagccctg gcaccctgcc cggataccag cttccctgaa
     2161 acaactggca tcaggtgcgc ttatcgtcaa ccgttgtgcg gtatgccagg agcacgtact
     2221 gatccatcag atccgtcagt gctgcgctct gcttctgacg gttgatgatc cattcctcca
     2281 gctctttttc tgatttccgt ttcagattct ccgtccagtc gtcaaggcag tttccggtca
     2341 ttaaatcaag ccagcaaagc cagaccagtt tgaggcgcaa ctccgtgcca ccaaactgac
     2401 ggaagaggct gatcagttta tttgccagcg aacgctgcct cagacgttca cattcagaca
     2461 ggagaatgcc ttccagcatc ggatgatgaa ccagcagatg ttcactggtg cataaattca
     2521 ctatcagatc atcatccagg ggcggcgtgg tccgtttgtc cggaccataa atcagccggt
     2581 gcagactctg gggtacgatg gcaatctccg gtttttcacc attgaatgac acccacccgg
     2641 gcgtcagatt atggaagtag tagatattcg gatgacacac gccccaggat acggcgtgcc
     2701 gggtgaagcc ggtcagccat ttctggtagc tttcgtggag cagctcgtaa cgggtctgat
     2761 actgtggttt cattttcata ttcctcacgg gggataacag gcagcagaac gccgccggca
     2821 ggccggagac tgcgtctgct gcagatgaac ggttaagaat tacggacggg actgtgcaat
     2881 acgcgcctgc agccaggctt ccacttcgct tttcagccat cgggagctgc ggcccatttt
     2941 gatgggcgca ggaaagtcac catccttgat gagtttgtaa aaccacttat cggtcaggcc
     3001 ggtcagttga gtgataaatg ccatgtcgac catctggtca tccatcagtg aaactggggt
     3061 gatcatgaga atgttcctcc ggatgttgtt aaaccagccg gtgtggtcat gaaaacaacg
     3121 caccaccttc accggcataa caaccgggga gaaacgtaaa aaataatcgg gaatgtgacc
     3181 gccaggagat aaacggggag gttacggtgg ctgagccgta ccggtatgcc gggtggttgt
     3241 tacgaaacga tgcttccgca cgcgcgcgcg ataaagaagg gatgattttc gtgaggaaga
     3301 ggggaaaact ggcgttctga taattgcaga gttatttacg gggcggttac gatggggcag
     3361 accgtgtccg gtgggcaggc gacaggtatc accgatggga taagactgtc tgattggtat
     3421 tataaatata taagagagag caaacacatg agatcaaata catgagttca tatatgtgaa
     3481 ataaacacgg tgagtgtgat cagtgggcat gatgagaacg cagtagcaac agactgcgct
     3541 atccctgata gctggcgtat aacctgcgta tataaaacgt accagcaacg cacgtgaaca
     3601 gagtgcatta agcatccgtc gttgacaccg acctggtgag tgacagtctg agagaccggt
     3661 taagtggtgg tacgggagta atgagtttgg ggagggtacc agaatgtcag tctggtacgg
     3721 tatgctgaca gtatcacgtt aattgcagca taaagtgatg agaggctaat ggaagttaaa
     3781 accagagcag ttccgtcgtt gttcatcccg ggagacactg gaaaaagtga tttcccacac
     3841 gcgttataag cttactccag cggagatgga agccttcaac tctgcggtcg atcaccgact
     3901 ggcagaactg acaatgaaca agctttacga tcgtgttccc gcttcagtct ggaaatatgt
     3961 catctgagcg ttcggtaatc acaactgcat cgcgccaggt gttatccgct ttgtgattct
     4021 tattggctct tctcaggtta ttaaaagctg aatatgttcc tgttgtcggg aaccaggata
     4081 ataccgggaa cgttaagcgg tatagcgaca acaaagaatt cgaaatcaaa agggaatggt
     4141 gtacggaggt gtaggcggta atgggccgat tttcgggcat taccgacctc tgctgaggtt
     4201 ttctcgtatt tatcggggag ctggtgcgtt actgtcactg tctggcacag caaacgcaga
     4261 ggtggcgggg cgctctgctc cgctgactcc ggctaatctc tcccctttta cctactgcaa
     4321 cagtacaaat acactgacgc ctgaaacgac agccagaatc agcacaggca gtaacgtcag
     4381 tagacaaagc agacgatcgg tatttcggta ataatcgaag ggatctgctg acacgtagcc
     4441 cgggcaacat tccgtattat gagggatggc gttatcacaa tccctgcaca ccgttttgcg
     4501 tatcaccatc acgtcctcct cacaatctta tccgtaaccg gctttttgca ttaaaaaaac
     4561 aacgctgaaa attcagccgt cagtccggag acgacagtcg ggttatctca gagtgcctga
     4621 gatattgcct ggttggagat cacttcaggg actctgcgca ttttttacgg cgtggttaat
     4681 ccgtatgaac gaaaccatac ggagatttaa ccatgaccta caaatacaac cccttctggc
     4741 agcaacgtat tcgtgagacg gtgcggcacg cactggatgt tcatccccgt ctgacggcat
     4801 tgcgggttga tctgcgtctc ccggatgtac cggcagcaac ggacgcggct gtgatatccc
     4861 gcttcatcaa tgccctgaaa gcccgaatcg acgcttacca gaaacgtaag catcgggaag
     4921 gtaaacgcgt gcattcctcg accctgcatt acgcctgggc ccgggagttc ggggagctta
     4981 aaggcaaaaa acactatcac ctgctgctgc tggtcaaccg ggatacctgg tgtcgtgccg
     5041 gcgattaccg cgctccgggg tcactggccg gaatgattaa acaggcgtgg tgcagtgccc
     5101 tgggagtgga tgtcgggtgc catgccgcgc tggtgcattt tccggcctgg ccggcggtgt
     5161 ggctggaacg cgatgatgat gctggtttac gggatatgct gaagcgtgct gactatctcg
     5221 cgaaagagta caccaaagtc aacggtaccg gtgagcgcaa ttttggctgt agccggagtt
     5281 gagcacgact ggcgcgccaa tatccggcaa accaaaagta tgggttgttg tctggcggca
     5341 acgatgtatc cactcgccca ctgactgttt tttacgtcag tgttccattg tgtgacaacc
     5401 acagtgctgt aacagcaacc agcctgcttt acccaaactc cagtccagat tttaactgaa
     5461 ccgccattca caccctgacc acgctgcccg taccgggacg gctgttgcct gcgtgtcgtc
     5521 tggcggctga ggaatatgac gatgtacgca aaatcctttc ttgcacttga tggcaacggg
     5581 cgtctgacgg gtgcccgtac cgcacagact gcaccttacg atcgctacac ttgccacctt
     5641 tgcggcagtg cactcagata ccacccgcaa tacgacactg aacgtccctg gtttgaacac
     5701 actgacgacg ggctgactaa gcacggtcat gagtgccctt atgtcagacc ggaacgcagg
     5761 gaggtacgct tgattaagcg tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt
     5821 aaagccagct ggcactgcag acaatgtcac cacgattatt atggggagca gtactgcaca
     5881 aactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat atgcgaattc
     5941 tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa gaagctgcga
     6001 agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta acacttcagc
     6061 ggggcaatga cggacaacca ccgtggtttg aacatgacca gatga
//
LOCUS       DAZFAH010000233         6074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFAH010000233 DAZFAH010000000
VERSION     DAZFAH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.235"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1295
                     /gene="dgcT"
                     /locus_tag="ACHJWB_004294"
                     /pseudo
     CDS             1..1295
                     /gene="dgcT"
                     /locus_tag="ACHJWB_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(1336..2498)
                     /locus_tag="ACHJWB_004295"
     CDS             complement(join(1336..2237,2237..2498))
                     /locus_tag="ACHJWB_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO4315815.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRDQGYPFNVKTVAASLRRQGLRTKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            2565..3092
                     /locus_tag="ACHJWB_004296"
                     /pseudo
     CDS             2565..3092
                     /locus_tag="ACHJWB_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            3089..4075
                     /gene="ycdU"
                     /locus_tag="ACHJWB_004297"
     CDS             3089..4075
                     /gene="ycdU"
                     /locus_tag="ACHJWB_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO4315816.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNYKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(4268..4404)
                     /locus_tag="ACHJWB_004298"
     ncRNA           complement(4268..4404)
                     /ncRNA_class="other"
                     /locus_tag="ACHJWB_004298"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            4622..5824
                     /locus_tag="ACHJWB_004299"
     CDS             4622..5824
                     /locus_tag="ACHJWB_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4315817.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         1525 a         1268 c         1496 g         1785 t
ORIGIN      
        1 tggttttggt gaatagttgg tttaatcagc caggcgttga ggaagttgtt ccacgatcaa
       61 cctatctgat ggtaatgatt gctttatttt ttatcgatac tgttgcattt atttttatgc
      121 agttgtattt catttatgac cgtaggcaat tttcaaactg tatacttagt ttggcttttc
      181 tgagttgttt gatttacttt gttaaaaccg tcattatcat tcagcaaatt attgaggggc
      241 gtttgacaag cagtgttgtc caaaatgaca ttgcaatcta ttatttgttt cgtcagatga
      301 gtttgtgcat attaatattt ctggcattgg tgaataaagt tagtgaaaac acaaaatagc
      361 gcaatttatt ttccaaaaaa atgactttgt gcataagttt gttttttgtt tttggggggg
      421 cgatagttgc tcacatactt tctagtcatt atgagagcta caatttacac attgccgagt
      481 taaccaatga aaatggtcag gtggtttgga aagcctcata tgttaccata atgattttca
      541 tgtggttaac cttgctttcg gttaatctat actttaatgg tttacgctat gatatctgga
      601 atggagtgac ggtgattgct ttttgtgccg tactgtacaa tatctctctg ttatttatga
      661 gtaggtatag cgtgtccact tggtatatta gtcgcactat tgaagtagtc agtaagttaa
      721 ccgttatggt aatatttatg tgccatatat tcagtgcgct acgagtaaca aagaacattg
      781 cacatcgcga tcccttaacc aatatattta acagaaatta tttttttaat gaactgacag
      841 ttcaatcagc atcagccaaa aaaacgcctt attgcgtcat gattatggat atcgaccact
      901 tcaaaaaagt caacgacacc tgggggcatc cggttggcga tcaggtgata aaaacagtgg
      961 tgaatatcat tgggaaaagc atacgaccag atgacctttt agcgcgcgtc ggcggcgaag
     1021 agtttggcgt cttgctgacg gacatcgata ctgaacgtgc gaaagcttta gcggaaagga
     1081 ttcgggaaaa tgttgagcgt ttaactggcg ataatcctga atacgctata ccacaaaaag
     1141 tgacgattag tattggcgct gttgtgactc aggaaaatgc gttaaacccg aatgagatct
     1201 atcgactggc tgataacgca ctttacgagg cgaaagagac cgggcgtaat aaggtggttg
     1261 tgagggatgt ggtgaatttt tgtgagtcac cataaagcgg cattttgatc ctacccacgt
     1321 aatatggaca caggcctaag cgaggttctt gttttcaaat tgttccggac tgaggccgcc
     1381 acaccaactg tgccgccgcc accgattgta atcacattcg atataattaa acaccgttgc
     1441 ccgcattatt tcccggctga taaagtgttc tccatggata cattccactt tcagcgaatg
     1501 aaagaagctt tccacgcagg cattatcgta gcagcaacct tttgcgctca tacttccacg
     1561 cagattatgc cgcttcagtt gcgcctgata atctgctgaa cagtactggc ctccacggtc
     1621 cgtgtgaacg ataacgttcc ggggcctctt acgccgccac agcgccatct gcagggcatc
     1681 gcaggccagt tgcgccgtca tgcgtggcga cattgaccag ccaataacgg cacgtgacca
     1741 caggtcaatg accactgcca gatacagcca gccttcatct gtacgtaagt acgtgatgtc
     1801 tcctgcccac ttctggttcg ggccactggc gtaaaaatcc tgctccaaca gattttctga
     1861 cacaggcagg ccgtgtgcgc ggtagctgac cgggctgaac ttccgggagg cctttgtcct
     1921 cagtccctga cggcgcaggc ttgccgccac ggtttttacg ttaaaggggt aaccctgatc
     1981 acgcagttca tccgtcaggc gtggggcacc gtaacgctgt tttgaccggg taaaagccgc
     2041 gaggacaacg ctgtcgcagt gttggcggaa ctgctgacgc gtgcttatcc ttgtccgccg
     2101 ctgacaccac gtataccagc cgctgcgggc cacccggagc acgcggcaca ttgctttgat
     2161 gctgaactca gcctgatgtt tttcaataaa gacatacttc atttcaggcg cttcgcgaag
     2221 tatgtcgcgg ccttttggag gatagccagc tcttcatccc gttctgccag ctggcgtttg
     2281 agacgtgcaa tctcggtaga catctccagt tcacgttcag aagacgtctg ctgattttgc
     2341 tgtttactgc gccagttgta gagctgtgat tcatacaggc tgagttcacg ggctgcggca
     2401 gtaacaccga tgcgttcagc aagcttcagg gcttcactgc gaaattcagg cgaatgctgt
     2461 ttacggggtt ttttactggt tgatactgtt tttgtcatgt gagtcacctc tgactgagag
     2521 tttactcact tagccgcgtg tccactattg ctgggtaaga tcaggcggtt aaaggcatca
     2581 accgcgaaca actgcagacg atgattacgg aatcaggcat tgcgctggat attagctgtg
     2641 ataacacgcc gcgccagcag gttattgggg gaacgcaggc tgcgttgaat gaatttgcca
     2701 cgctgctgat ggccgctgga tacgaaccgg ttaaactcgg tgtgagcggt gcctggcaca
     2761 cgcgtttgat ggaagacggc gttcaggcaa tgcgcgatta tcttgccgga ctggatatcg
     2821 cttcgccaga gcatcaggtt ctgatgaacg taacagcaaa aagcgaggtc gctccgtcaa
     2881 ttatcaaaga aaatctctca ttacacttaa ctcatacggt gaaatggact gaatctttcg
     2941 atacatttct gaatatgcca accccggtag ctttccttga aataagtaat aagccctatt
     3001 tggggaatat gttaaatgat tttgctggcg tggatcagca acgagttatg cattgtcgta
     3061 aagcatttag tgatgcaaag gtattcaaat gattcctgat tatttaactt ttattcgctt
     3121 tcaggataaa cgaaatctga tatacattta tgctattgga cttattctga taggctttta
     3181 ttggaagaat gcagggttta cttttccatc agaggatatt ggtgtagtta gtgggattct
     3241 ggccctggtg ctgtataatt ttatttttga tctcaaggcg tactgggctt ataaatgcgt
     3301 cacgaagaat atcgattttt cgtggtttaa gaaaaagcag aactacaaaa tagaattatt
     3361 tcttacacaa cctctggtgg caggatttct gtcgttaatc atgttgagtg caatgagttg
     3421 ggggctatac cagcttctac cctcgttata tgcgctgttc ctgatttcgt tacttgggcc
     3481 gttggtcatc tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc aggtcgctat
     3541 ttcagtagcg aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc tttcggtgtg
     3601 catctcaacg gttgttaacc tgcttactat cagcccgttg cgtaacagtg attcttttgt
     3661 gacagagggg cagtggttaa cgtttaaatc gataattgca ttgctcattc tttgtggcgt
     3721 agtgttggcg attaatctgt tttttctgcg cttctccaag cggtacgctt ttctgggcag
     3781 gctttttttg caggaaatcg atctgttttt ctccagtgaa aatgcgttgt cgaccttttt
     3841 tgccaagccg ctttggcttc ggttattcat attgctggtt attgaaatga tgtggattac
     3901 gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag cctatttttt
     3961 actctgctat gtaccgtgct taatttacta ttttttccat tgtcgattcc tctggcataa
     4021 cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata agtgaggaag
     4081 ttacaggaag tgaccagata acgcatatat gttctctact atcttgcgca ttttgattgt
     4141 tgactgagta accagacagt tgatgtgcac aatttcccct cgccctaaca gacgtgggcg
     4201 ggggcacccg gtgtgtgcaa tatacgaaaa aaaagcccgt actttcgtac gggctcttct
     4261 ttaaatatgg cggtgagggg gggattgact cgcttcgctc gccctgcggg cagcccgctc
     4321 actgcgttca cggtctgtcc aactggctgt cgccagttgt cgaaccccgg tcggggcttc
     4381 tcatcccccc ggtgtgtgca atatacgaaa aaaagcccgt actttcgtac gagctcttct
     4441 ttaaatatgg cggtgagggg gaccgcctga aataaatctg acttaatcca ggggtgttat
     4501 ataatttcat tataaacaat gtattacact ttttgttgtc cgatattatc taatgtattc
     4561 ttagtgaagc gagagactaa tggcatactg gatggcatac cagagtgacg ggaattagcg
     4621 tatggcagta ttgacggata cgaaagcaag acatatcaaa cctgatgaca aaccattgcc
     4681 ccatggggga attaccggac tgacccttca tccttcttca gtaaaggggc gggggaaatg
     4741 ggtttttcgt tatgtaagtc cggtgacaca aaaaagacgt aatgctggat tgggaactta
     4801 cccagaggtc agtattgctg aagctgcacg tactgcccgg ataatgcgag agcaacttgc
     4861 tgcaggtgat gatcctctgg agattaaaaa ggctgaatct gagaaagtcg ctatcccaac
     4921 atttgccgat gcagccaggc gtgtacatgc agaactgtct cctggatggg aaaatccaaa
     4981 gcatgtaagg cagtggttat cgacgcttga gaattacgcg tttcctcaac tgggagcaaa
     5041 aacgctggat tcgattacgg ctgcggacgt ggcagaaaca ctgcgtccag tctggttaac
     5101 cttgtcagaa acggcaagcc gggttaaaca gcgcattcat gttgttatgc agtggggatg
     5161 ggcgcacggt ttttgtgtag caaatcctgt tgatgtggtt gaccatttgc ttcctcagca
     5221 gacaagagga cgtgatgaac accaacccgc aatgccctgg aggcagttac cgctttttgt
     5281 ggcgaccagt gtgtataccg atgaacctta taatgttacc cgcgcactgt tattaatggt
     5341 gatacttaca gcaactcgct cgggcgaagc caggggaatg cgctgggctg aaattgattt
     5401 tcataagcgg gtatggacta tacctgcaga aagaatgaaa gccaggatac agcatcgtgt
     5461 tcctttatcc cggcaggcta tttacattct ggaaaatata cgtggcctgc atgatgaact
     5521 ggtgttccct tcacccagaa agcagcagat cctttccgat atggtgttga caagttttct
     5581 gcgtaaaaag aaagccgtca gtgacattcc ggggcgagtt gccacggcac atggttttcg
     5641 ctcaacattc agggactggt gtagcgaaca ggggtattcg cgggatctgg cggaaagggc
     5701 gctcgctcat acgctgaaaa ataaggttga ggcggcatat catcgtactg atctactgga
     5761 gcagcgtgta ccgatgatgc aggcatgggc ggattatgtg atgtctcaaa ttgtgaataa
     5821 ataatagtga aaatgtatat tgtacgatca tgatttgata tgtgatcgta gcagtagtaa
     5881 ttaagaaacc gcttttatgt caaatgtatt gagctgataa ttatcagatc gtcaggaaag
     5941 cgtattttga ttaacagggg attacttaac aatccccttt ttataaaaaa taatttgcct
     6001 gtttaactct tcactcctgc tgagactgaa ttattcctag tgcgtcataa atcagtcttc
     6061 tgcgtagttt attg
//
LOCUS       DAZFAH010000234         6024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFAH010000234 DAZFAH010000000
VERSION     DAZFAH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.236"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..581)
                     /locus_tag="ACHJWB_004300"
     CDS             complement(<1..581)
                     /locus_tag="ACHJWB_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4315818.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIIYRLS"
     gene            850..1050
                     /gene="glgS"
                     /locus_tag="ACHJWB_004301"
     CDS             850..1050
                     /gene="glgS"
                     /locus_tag="ACHJWB_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4315819.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(1093..2157)
                     /locus_tag="ACHJWB_004302"
     CDS             complement(1093..2157)
                     /locus_tag="ACHJWB_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4315820.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(2159..2907)
                     /locus_tag="ACHJWB_004303"
                     /pseudo
     CDS             complement(2159..2907)
                     /locus_tag="ACHJWB_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(2923..5427)
                     /locus_tag="ACHJWB_004304"
     CDS             complement(2923..5427)
                     /locus_tag="ACHJWB_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4315821.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPSPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVIITTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            complement(5487..>6024)
                     /locus_tag="ACHJWB_004305"
     CDS             complement(5487..>6024)
                     /locus_tag="ACHJWB_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4315822.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKLGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSK
                     DATPGNVYAQADYKIAYE"
BASE COUNT         1718 a         1206 c         1260 g         1840 t
ORIGIN      
        1 gaaagtcggt aaataattaa cactttgtct cctttagtga aaaattttcc ttcttcaggc
       61 tccagtagta aataatgtat ttggccaaat tgatcggtga gttttccttc gcagggatta
      121 cctgaagtgg cttgatgacc ggtaattaat gccatgctac caatatattc ttcttctgta
      181 atagctgaac tatggtcgcg tggaatccag ggcgcaacta ttttaccggt ataatgcacc
      241 gcaataatcg taaacagtaa actgacagga accacgaaca ggttcgacag tggtgattgc
      301 cagaccatga tgcaggcatg ctgcaacagg atgccaataa gaccaaaaaa accagccagc
      361 aagcagagaa cgacgagagc tggcaatctt ccgatattga gataatgaag tgcctggcta
      421 atatgacctg tggttatgga atcataatgg tcaagatgtg catcaagagc accggagagc
      481 atgtgaccgc agataagcgc taaaatttct aaaaggccga tcaacagtac aaaggaaata
      541 gcgaaaagat aaggggtatt atagtcggcg aataaaatca tatgtcactc caatgtccat
      601 ttagtgacgc aatatttact ttgggatagt ttccttgctt acatttatct ttgttaataa
      661 taacacatat tagagaagtt ctggaagaga aagtttttta atatggtttt ttatttttca
      721 ctaaaaagtg tgatcgggga caatatattt acgcacgtta tgtttaaagg cactacactg
      781 attggggaat actgaaatca gaaaaagcat atggccttaa aaggtatgcg ataaccggag
      841 gatgtgctta tggatcatag tcttaattct ttaaataatt tcgatttcct ggcgcgtagt
      901 tttgccagaa tgcacgcaga aggtcgcccg gtcgatattc ttgccgttac tggtaacatg
      961 gatgaagaac atagaacctg gttttgcgca cgttatgcct ggtattgtca acagatgatg
     1021 caggcaagag agctggagtt agagcactga tataacgggc ctgatggccc gttttagtgt
     1081 ttgtattaga tgctagttat aggtgatttt gaggatgacg tctttactaa acggtccctc
     1141 aacaacagtc tctgccggat tcgcactaag ttctgccctg aatggtgcgg tgaccgaagg
     1201 tgcactagac gatgaaaaag aagcaaattg tgagaagcgg ttaaattccg ttgctgttcc
     1261 atctttatga aatatcttca atagcagtcc attacccata tccagatggc tataatcact
     1321 caaaatatcc gtagtgaaaa agctccctaa aatattgaat tgctcagtac aggccgcacc
     1381 aggatctttt gtcatgacta cactaaacgt ctcactaacg gtggtatttt taattgttcg
     1441 ggaatttatt ttaccaaagt caacaacgcc atcagctggt tccagtgcga cattaacaaa
     1501 gcagtcaagg acatgaatat tatttaatcc acgaatcctg aaggtcaaat ttttgtctac
     1561 ttctatagct cctgcgccac tcataccttt aaactctaga gcgatatatt catcaggtaa
     1621 caccaggttt ttattaactc tattggcatt ttttgctaat ttaatataga gttgtacgcg
     1681 agctggaaag gttactgata attgaggctc aggacctcca ctacagacag cggggttttt
     1741 atagtgatta ttaaaggtac ccgctccttt tatatcaatg cattctggtg ctgcgatagc
     1801 attaggggca gtcgaatcat aatcaactcc attgtatgtt acgccaaaaa tataggagtt
     1861 ctgattataa aagtcctgac cgcctggtaa aaacatcatt atattagtcc aggctgatgg
     1921 gtattcttct ccaggtgatg gggcaggtaa tgcattatta cagaagacat tcataaaata
     1981 ctcactgctt tgccagatct tatcaccttt tttaccatta ctggggatag aaattcttgg
     2041 tacgtaacca atgactacgg tatttccccc ttcatgttca gcatagcagt ccattgccca
     2101 tacaggtaat gcactaaaac ctataataaa tataattaca attaataagt agcgcatatt
     2161 atttatctcg tttggctacg gaaacattac aatagttatt aaccgtcggg cagtttattt
     2221 cataaaaact taatccacca aaatcattga tataaccaac ctgaaaagct gacggaacat
     2281 ttttaaactg cgtatctgca tggctataag gggttaccat taaactttgc gtttgtggca
     2341 acattgtgat tccgtttgtt ccgatataac ccagcgtaac gtgataaggc atcttattgt
     2401 taaagcggat cgaaccatca tttctacgga tgatttcaac tttttcgtaa gacattaccc
     2461 catcatcttc taattcaata gcttttggtc gccagaatag tttcaatgcg tgctgcatag
     2521 tgacttgcat cacatttttt tatctgtttt tggcggtatt tctcgcacgt taaaatagaa
     2581 gagcgtttct ctgtcattag gcagttgagc aattttttcc tgacttatca ttcttatttg
     2641 aatttgctca ctgggattta aacgtaccat agggggaacc gtgacaataa agtcacgtga
     2701 tttattgcct ttcgtatctt ctatccatgt ttgcgccaga tagggtagat ttttgctttg
     2761 attagtaagt tttaatgctt cccctttatc tgactcattc attatgatac gggtacggtc
     2821 cgggatgatg gcaccccagg caatgctgct catgcaggcg agtaatactc ctgctgcaat
     2881 gagattcttt gtgtttaagt agcgcattag tttcatcctg aattaatttt ggctaataca
     2941 cggtaaaata accgttccgg tggttaagtt gctgctattg ggtggttgaa taaaacagga
     3001 tttatcaccc cagcgtaaag taagtttgct gtcatcctga atgccgttga ggtatacaaa
     3061 gccgccatca gcaaccagtc ctaattcttt atggctggtt ttatctttta cgctaatccc
     3121 cagtggtggg tgactaccat ctgccaggcg aataaccccc atgatttgat agcccttgcg
     3181 ggtatcaagt ttggcgtagc caatagcacc ttcggttaag gtggtgataa taaccgagtt
     3241 ttcgatatcc acattttctg gaagatttcg ggtatcaacg cttaatgatg ttgttatgta
     3301 actgctgact gatggaataa ctccgatgcc ataccgatta gttaccgcgc gtttattgtt
     3361 caacggaata tcttctgcac catctgcgtc gatcataaaa cgcgagtcag cactaccact
     3421 atagtcatgg aaagcggctc cgtgacgagt cgccgtgaat gaaccattcc aactggcatt
     3481 cagagaataa taactgtctc gctgtgatgt cccggagaga tagagacgac cgttctcggt
     3541 attatgctga taactggcgc gtagtgacgc tttcatatct ttgaattcat cattgtcgcc
     3601 tgaagcagaa acgctccagg aattatttcg atcggaagag tcataccacg aagcagtatg
     3661 cgaaatcttg ttatcctgat ttcgttgcat accatagctc aatgttctac tggttcccca
     3721 gggaatcgaa atatttaggt acagttgatc ctggttgtct tcttcccagt ttattcggct
     3781 aaatgttaga gaagtggaga cgttttttaa tggcccgata tcaaaatcgc ggctaagtga
     3841 taatgaataa ttcacattac tgctggcgtc ccagtaagta ttacgagcca aacttacgta
     3901 tgtatttaac cttaatgatt caagatactg gttaaacgtg acaatataac tctctttttc
     3961 attctgataa ttcgtgtaat ggttagtgtc attgatatat tcattcattg tcacaaagtt
     4021 tttatcagag aaacggtaac cagcgaaagt gagctggcta ccggtagatt caaaacgttt
     4081 tgaatagtta gcgcggtagc tataacccgt ttctttatcc tgattatgca actgagcatc
     4141 agatcgtgtg acatcaaaag ataatgaacc caatgagtta aggttaaagc caacgccggc
     4201 agccagagat tgataatcac ggtttgttaa aactgaacca ccatacaggg atacattgtt
     4261 cagccaaccc catgagaatt caccagtcca gaagaagggt tgttgtaagg aatcaccacc
     4321 aacgctggtc ggtttaccca tagcagtggt gtagcgaatc tgccctttac gcgtcagata
     4381 aggaatacta ttagatgcaa cttgccattg cgtgacttgc ccgtcctctt cttcaacctt
     4441 gacgtccagt tgcccctgta atgtattgaa taaattagta atagcaaatg ggctaggagg
     4501 aacattttct tgataaataa tacggttgtt ctgtgagaca gtgacctttg cattcgtttg
     4561 tgcaacaccc gtgatttgcg gtgcgtaacc acgtagatca ggcggcaaca tattctcatc
     4621 gcttttcaac gaaacgccag aaaaatggaa agagtcgaaa atagaggatt cagtatcgta
     4681 ttgacctata gttagttttg cattaattgc tgggattggg cgaaacaaat aaatacgcgg
     4741 aaaatccagg ttgttcgcct ggcttttgcc attgttaaat gactgatcgt actggtaatc
     4801 agtacgcagg cgccaggccc caagattaac cccagcctgc ccataggaac ttatattttg
     4861 tgaattatcg ccctgatgtg gtgcataatg agaggcatat aaattgtagt caagaaatgc
     4921 cccagcaata ccatgatccc aaagttcagg aggcgtccag tttgcatctt tgtattttaa
     4981 ccatgcctga ggtgcagata tggataattg cattttccct ttatcgagat aagttgtaat
     5041 ttgtttttct ttttcgatag ggtaacaacc atcgatcagg ttttgctgag ccacttcaat
     5101 aatctcatca gtaaagccta ataatgttaa ttgctctgga gtcagacaaa ctgcagtttg
     5161 gttatcaatt aatacccatt gcaggtccag atggattggc gtccttttat tattaataag
     5221 agcgtttaat gagtatttac ctacgggtaa ttggccatca gtttcaaatt gggaaaggtt
     5281 aacgttttca cgatcttcgg cttcgattaa atctttgttg aattcaacgg cataagctga
     5341 cgcacaacca atgatgagaa cggataatga acaataaata tttttaatca attcgcttat
     5401 tgtggttaat tttaattttt tatacatctg ttccacttat cattaatccg ttaatgatgt
     5461 ggccgggcga acccggccta atgtgattac tcgtaagcaa tcttatagtc agcctgagcg
     5521 taaacgttgc ccggggtagc atctttgctt ggggattcca tacgagcttt gaagttcagt
     5581 tcctgatagc tgttagtatt gttgaatgta attactaccg gagtacctaa agtcacttcg
     5641 ccgctctggc tatccagaat acgtacgcca acgcctgtgg caccaccgat ttcagtttct
     5701 ttggtgtttt tcagcaggtc agtctgaccc gtcatagtat tcgctgtggt gaaagtaacc
     5761 ttcgcttttg aataagtggt tccaccctgg ctcaacatgc agtcttgcag gtgaatagtg
     5821 aaaggctcag ccaaagattt ctggtcgctt ttcagaacgg tatctgctac ttcacccaga
     5881 tttacagtta agtcttcgtc acctagttta attgaacatg gagcagagat aacttcacct
     5941 ttaaatttaa caatgccgtg accttcgtca gcaaatacgt tattggaaag aatcattgct
     6001 acgccagcaa caagaagtga cttt
//
LOCUS       DAZFAH010000235         6007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFAH010000235 DAZFAH010000000
VERSION     DAZFAH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.237"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3541)
                     /locus_tag="ACHJWB_004306"
     CDS             complement(<1..3541)
                     /locus_tag="ACHJWB_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO4315823.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQ
                     RYHYDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLS
                     EGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPP
                     VEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQ
                     LQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIP
                     YATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIR
                     TDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWM
                     SLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSL
                     AEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRVWLAQCGLTVEQLARQ
                     VEPEYTPARKVHFYHCDHRGLPLALISEDGNTVW"
     gene            complement(3609..5716)
                     /locus_tag="ACHJWB_004307"
                     /pseudo
     CDS             complement(3609..5716)
                     /locus_tag="ACHJWB_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1061 a         1913 c         1660 g         1373 t
ORIGIN      
        1 tccacacagt attgccgtct tcgctgatga gcgccagcgg caggccccgg tggtcgcagt
       61 ggtaaaaatg aacttttcgc gccggtgtgt attccggctc cacctgtctg gccagttgct
      121 ccaccgtcag cccgcactgc gcaagccaca cccggctttc actgctcacg cggtctgccc
      181 ggatttcttc ctccagtctg tccagcagcc gcaccagttc agccggaaat accacgccgt
      241 gcccgtcctc gctcccttcc tgctgcagct tctccgccag gctgcggcac tgcgctttct
      301 cccgttcacc gttgtctgtt tcgatgcgga tgagcggcgc gaagctcccc ggctggtata
      361 ccgtctggat acgcgtggtg tcggtctgta ccgtcgtcag cctgtcgccg tcccagccgt
      421 accatgtcac ctccggttta cgcgacagcg acatccaccc cgtcaggtca cgctcccgcc
      481 gccatacccg ttttcccgtc cggcggccca gcgggtcata gaggtagcgg ctctcgacca
      541 gtggctcgcc atgctgtatc cgcgtgtgga acaccaggcg gtgctggctg tcgtagtggt
      601 agtggtgggt ccgctcgtcg tccgtccgta tcacacccgt cgggatgcgg tccgtcttct
      661 ccgtcagtct gccgtattca tcatagtggt aaacatagtg cgcatcctct gcgatgcggt
      721 tatccggcca cgccgtgagc gtgctgtccg ggtgtagctc cgggtccggc aggcggtttc
      781 ccgccgggtc ggtggcatac gggatgcgga tgtccaggtc cggtgcgagg gtgcgcacgc
      841 tctccagcct gcccgtggca ctgtagccgt attcccgcgt ctgtcgcggg ccgctgatgc
      901 gcaccaggtc gccgttgtca ttccacccgt agtcacggtc atacaccagg ctgttcaggt
      961 gctggctctg taactggcct gcgggggtgt atgtgctggt cagttcatat gcggcattac
     1021 tgcctgccct gctgccgaag ctgcgcaccg tctcacggtg cagcctgtcc cgcgtatact
     1081 cgaccagcgg cgtgtcgcca agcttcatgc ccgccagata accgctgccg taggtcagcc
     1141 actccaccgg cggcaggctg tccggcgtga cgcggtttgc cagcccctgt tcgttgtagg
     1201 cgtgtgttgt ctcatgctgc cacagcagtt cccccgtctc cgggttctcc accgtctgcc
     1261 gttcgccggt caggcggccc ttatcgtcgt agccgtagtg gacagcgaca cggtggcctt
     1321 cgctcaggtg gctgatgtct gtcagccagc cgtggtcatc atactgccac tgctctgccg
     1381 gttcgccgtt caccgtgcgg tgagtgatac ggtccgattc atcgtagtac cagagggtga
     1441 caagcccctc atcttcactc tgcgtgagtt ttccggtcag gtcataatga taacgttgcg
     1501 tccgcccgtc aaagccgccc tgctgtacca accggtccag cgcatcgtag ctgaagtcgc
     1561 tgtggctgcc gttctcgtta gtcagactga tgacacgtcc ggcagcatcg tactccatac
     1621 tgcgcgtcag cccgccctgc gtggtgctga ccgcctttcc ccacgcatcg tactgtgtct
     1681 cgctccggtt gccgtccggg gtgataacgg cagtcaggtc gcctgcggcg ttgtactcat
     1741 actgcgtttc acggccctgt gcgtctttca ccgaggttaa ccggccacgg ttgtcatagt
     1801 ggcggtaaag gctgatacct tcctcgcggt gaaccgccgt catctggccg aagcggtcgt
     1861 attcatagcg ggtctggtag cccgagcagt cagtgaacgc cagcaactgc ccgtagcggc
     1921 tccacgtcat ctgccgggtg ctgcccgtcg catccgttgt cgtcgccggt aactcactgt
     1981 gcgcgtcatc gtagcggtag cgtactgtct ccccgctgcg cgatgtctcc gataccagcc
     2041 tgcccggttc atcatattcc cggcggctct ccagcccgtc cgggtacacc accgccgtca
     2101 gctggttccc gtcgttatag taaaatttcg tctcccgccc gtccggtgtg gtgatgtccg
     2161 tgatatcgcc ggacaccaca ttcagaccgt actctgtccg ccgtcccgcc gcgtccgtct
     2221 gcgccgtgag ccttcctgcc gcgtcatacc cgctgtgcgt gacgctgccg tccgccagtt
     2281 cttttttcac cacccgcttc agcccggccc cgccttctgt atgcagcacc tcacgccggt
     2341 tcaggctgtc ggtgacggtg atgcggtcct gctcatactg gtagcggtaa ctcaggcctg
     2401 ccgggttaag ttgctccacc acccgccccg catcgtcgta gcggtagcgc atctccggcc
     2461 ttcccgcgta acggtgcgcc accatccggc ccgggtgctg cgcgtcatac gtgaaagcgc
     2521 gcacctgcgt attgctgcgg tcatataccg ccagcagttc accggcttcc gtatacgtgt
     2581 accgcaccag tggcgcagcg ggcaggctct ccgggtatgc cgggtcgtgc atcagccaca
     2641 ccgccgaaag acggataccg ctgtcagcgc cgtattccgt accgggcagt gtgtcgggga
     2701 aggaaggagc tgagagaggg cggggactgt caggggaaga taacgaagcg gtgtgcggtt
     2761 tacgggcctc ttccgcccgc tgtgcctgtg tggtcagtac cagacggaac tcccgcccgg
     2821 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgccggtacg
     2881 ccagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
     2941 ctggcagtaa gtcctcagca cccggcaccc gctcagacca ccccagtatc caccacggcc
     3001 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggtggcagtg
     3061 acgcccacag ccgtgccagc gtgtggccgt ccggctgcgc tgccttaccg ccacgcacca
     3121 gccacattga ctcactgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
     3181 tgctccgccc gccgttgtcg ttgagtatca gtgcatcatc gcgtatctgt aagcggatat
     3241 cagaaggcgc tttccagccg gggccgaaaa cgcccaccgg tgcaggcgtc ttcgtccggt
     3301 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
     3361 cgccgggctg tacttttgca ccgagcagcg ggttcaccgg gttgcccgag gtcatcccgc
     3421 ccggacacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
     3481 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
     3541 tgattcaaat tccttcttct gtttattttt aatgctggtt tgttactgaa ttccgaacag
     3601 gtacgggatc agtactctct catatccggc gtatcctgct tttcatcagg ggtcttcggt
     3661 ggggatttcg aggctgcaca gttccagtta atcaacagtg agtcaccatt aacctgctcc
     3721 ttaccctgca aatgaatttt tgtgccgttg agaaaaattt gtccgtcctt cgtcagcacc
     3781 agccttgcct taccacagca gagctccaga tgctcaccgg cggagtaaat cgcggtctgc
     3841 ccggtatcat cctttttcgt tttaccaacg gtgaaggtaa cgttatttcc gactttgacg
     3901 tcataaccca gcccgaccct caacgattta tgcaaaccga tctgcgagga ctgcatcagt
     3961 gccaccgagg tgttcataat gccacctacc gtcgtctgat acgccacgcc cacggtcagc
     4021 gcacgaacaa gtccaatcgt ttccacctga acagaaccca ctttaattat ctggttactg
     4081 cccaccgtct ctatctggtt aacacccacc gtctgtatct gattgttggt aaccgcaatc
     4141 atctgattgt tgccaatggt ttcagcatgg ttgtttatca catcagtggt gcggttattc
     4201 agcacctcgg tgttcatgtt cttctgcgcg tggatgtagg cctgttcttt ccctgtcgca
     4261 tcgtcaaact tcagttcatt aaacccgctg cccttatagg ttttcgaacg aatggtcatc
     4321 tgcgtcttcg tccccggcag gctgccgggt gtgcggtttt cctggtggta ggtacgcccc
     4381 ataataatcg gctggtccgg gtcgccgttg aggaagtcca caatcacctc ctgacccact
     4441 cgcggtatcg ccaggttgcc aaagccggtg cctgcccacg cctgtgccac acggatccag
     4501 catgaactgt cctggtttga cgggttatag cggtcccagt taaatttcac ccgcacgcgg
     4561 ccatgttcat cacagaagat ttcctcgcct gccggtcccg tcacgacggc gctctgcggg
     4621 ccgtccacca gcggtttcag caacggctgt ggccgccatg tccggtctgc cggtattacc
     4681 gcaaagtggt tattcagcgg gtacctgaac cactgcgtcc cggcaccgcc tgcggctgct
     4741 cgccgtgcag ttcacttgcc accacctgcc attcccggtt caggttcgcc tgcggatgcc
     4801 ccgtcagcgc aattcgccgt cccggccata tctccggaga acggcttgtt ccgcgcgcca
     4861 cttctgcgtt gtttcgccag ccatccatct gccagcgggc aaagttctgc ccgtgggcac
     4921 ccttgaaacg tccggggtag tcatacactt catactgtgt gcgctggtaa tcctggtact
     4981 ggccttcctg atcaaaacgc cctgcccagc cgggtcgttt aaaggtgtag tccttggtca
     5041 ccacggaaga agggcggatt tgtgcgctgt agcggaactg gctgatgcag agggtgctca
     5101 cctcggtacg ggtgttcgga ttccagggga tctcaaagga ctccggcaga taacgcacgg
     5161 tgtcgcacag gaccaggctc tggtcggtac ttttttgcgc gtgctcctca taaaagaaga
     5221 tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtttcgccg tactggacac
     5281 aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc acaccgtttt
     5341 ctttcaggat cgtagcgagg atgctttcga tgtcctcatt ctggaagata cggaagttct
     5401 ggcgcagccc tgtgcgccac aacggtgggc acaccttcat gctgtatagc atctggtttt
     5461 tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc tgtacgtcat
     5521 cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat tcaagggaga
     5581 gaaactgctg gctgaccaga gagagagcga gggaaaagag cgaagagagt gactggttca
     5641 aatgaaagga aaccaccgca aaagcatccg gtggcaggcc gtccacttcc agtgtgaaac
     5701 gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac atattgcatg
     5761 tacaacatgt cgggcggatg agataagcga gtcgacatct ggcgttaccg gggaatatca
     5821 gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag aaacgggtgt
     5881 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
     5941 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     6001 taatgtc
//
LOCUS       DAZFAH010000236         5971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFAH010000236 DAZFAH010000000
VERSION     DAZFAH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.238"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..454
                     /locus_tag="ACHJWB_004308"
     CDS             173..454
                     /locus_tag="ACHJWB_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4315824.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(553..1089)
                     /gene="ssb1"
                     /locus_tag="ACHJWB_004309"
     CDS             complement(553..1089)
                     /gene="ssb1"
                     /locus_tag="ACHJWB_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4315825.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1344..4166
                     /gene="uvrA"
                     /locus_tag="ACHJWB_004310"
     CDS             1344..4166
                     /gene="uvrA"
                     /locus_tag="ACHJWB_004310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4315826.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4201..4557)
                     /locus_tag="ACHJWB_004311"
     CDS             complement(4201..4557)
                     /locus_tag="ACHJWB_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4315827.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4561..4977)
                     /locus_tag="ACHJWB_004312"
     CDS             complement(4561..4977)
                     /locus_tag="ACHJWB_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4315828.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5088..5801)
                     /gene="aphA"
                     /locus_tag="ACHJWB_004313"
     CDS             complement(5088..5801)
                     /gene="aphA"
                     /locus_tag="ACHJWB_004313"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4315829.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1398 a         1564 c         1564 g         1445 t
ORIGIN      
        1 cctaatatgg ctaacaaaca atcaattgtg atatagttca caaaattaat gaaacaaaca
       61 gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg
      121 tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt tgatggctac
      181 cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa tgatgtttct
      241 ggccagcaca ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg cggtgatcag
      301 cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg gcatcacaat
      361 ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag agatgaatca
      421 gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca taacggaggc
      481 ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat aacctattgt
      541 tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct cgttagacgg
      601 cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact gattgccacc
      661 ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga tattaccacc
      721 tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc caacgttcac
      781 cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac gggtacgcag
      841 ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca cttctgccag
      901 tttgccgaac agcacaacgc ggtgccattc agtctgctct ttcatctcgc cggtcgcttt
      961 atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac catttggcat
     1021 gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt ttacgcctct
     1081 gctggccatg ttcgtgtctc ctgaaaaaaa atcgttctga ataagtgtaa acgcgcgatt
     1141 gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg
     1201 ccagacattg ttacacaaca ctccgggtat tgcattccaa tactgtatat tcattcaggt
     1261 caatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa
     1321 agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata
     1381 atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg accgggcttt
     1441 cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt
     1501 acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg
     1561 tcgatcatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata
     1621 acccgcgttc tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgttcg
     1681 cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg caaaccgtca
     1741 gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg ctgctcgcgc
     1801 caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg gcaagccagg
     1861 gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg
     1921 aactgcaaaa gaaacacact attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc
     1981 ttacccaacg tcttgcggag tcgtttgaaa ccgcgctgga gctttccggt ggtacagcgg
     2041 tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttctccgcc aacttcgcct
     2101 gcccaatttg cggctacagt atgcgtgaac tggagccgcg actgttttcg tttaacaacc
     2161 cggcaggtgc ctgcccgacc tgtgacggcc ttggcgtaca gcaatatttc gatcctgacc
     2221 gcgtgatcca aaaccccgag ctgtcactgg ctggcggtgc gatccgtggc tgggatcgcc
     2281 gcaacttcta ttatttccag atgctgaaat cgctggcaga tcactataag ttcgacgtcg
     2341 aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca
     2401 aagaaaacat tgaattcaaa tacatgaacg atcgtggcga tacctccatc cgtcgtcatc
     2461 cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacagaa tccagtgcgg
     2521 tacgtgaaga attagccaag tttatcagca atcgcccatg cgccagctgc gaagggacgc
     2581 gtctgcgtcg ggaagcgcgc cacgtttatg tcgagaatac gccgctgcct gctatctccg
     2641 acatgagcat cggtcatgcg atggaattct tcaacaatct caaactagct ggtcagcggg
     2701 cgaagattgc agaaaaaatc cttaaagaga tcggcgatcg tctgaaattc ctcgttaacg
     2761 tcggcctgaa ttacctgaca ctttcccgct cggcagaaac actttccggc ggtgaagccc
     2821 agcgtatccg tctggcgagc cagattggtg cgggcctggt tggcgttatg tatgtactgg
     2881 acgagccgtc tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc
     2941 atctgcgcga tctcggtaat accgtgattg tggtggagca tgacgaagac gcaattcgcg
     3001 ccgctgacca tgtgatcgat atcggtcctg gtgcgggtgt tcacggcggt gaagtggtcg
     3061 cagaaggtcc gctggaagcg attatggcag tgcctgaatc gttgaccggg cagtacatga
     3121 gcggtaaacg caagattgaa gtgccgaaga aacgcgttcc ggcgaatcca gaaaaagtgc
     3181 tgaagctgac aggtgcacgc ggcaacaacc tgaaggacgt gacgctcacg ctgccagtcg
     3241 gtctgtttac ctgcatcaca ggggtttcag gttccggtaa atcgacgctg attaacgaca
     3301 cactgttccc gattgcccaa cgccagttga atggtgcgac catcgccgaa ccggcaccgt
     3361 atcgcgatat tcaggggctg gagcatttcg ataaagtgat cgatatcgac caaagcccaa
     3421 ttggtcgtac tccacgttct aacccggcga cctataccgg cgtgtttaca cctgtgcgcg
     3481 aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga cgtttcagct
     3541 ttaacgtccg tggcgggcgc tgcgaagcct gtcagggcga cggtgtgatc aaagtggaga
     3601 tgcacttcct gccggatatc tacgtgccgt gcgatcagtg caaaggtaaa cgctataacc
     3661 gtgaaacgct ggaaattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca
     3721 tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag ctgcaaacgt
     3781 tgatggacgt tggtctgacg tacattcgcc tggggcagtc cgcaaccaca ctttctggtg
     3841 gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc
     3901 tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag caactcctcg
     3961 acgtgctgca taaactgcgc gatcagggca ataccattgt ggtgattgag cacaatctcg
     4021 acgtgatcaa aaccgctgac tggattgtcg acctgggacc agaaggcggc agtggcggcg
     4081 gcgaaatcct cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcgcatacgg
     4141 cacgcttcct caagccgatg ctgtaatcgt taaggccgct ttctgagcgg ccttttcctt
     4201 tcagagttgt accagcaatt tacgcttttc ttccggtagt aaattcactg cctgctgata
     4261 agacgcatcc accagataat agatttgcga atccggcagc gaaccgtcga gatacacggt
     4321 gctccagtgc gctttattca gatggcggct aggacgcaca tcgctgtgct gctgacgtag
     4381 cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt ctacttcttt
     4441 caccatcgca aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac
     4501 gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt
     4561 ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc gatgttcccc
     4621 cagccagatg ccttgccagg tgccggtctg aatacgccct ttatgtaacg gcaatacaag
     4681 cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc
     4741 atgctcataa tttccgttgt cgggaacggt gcggaggaaa aaacgctcca tgtcgtggcg
     4801 tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag
     4861 atgcagtaag ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt
     4921 taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg
     4981 ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag gataaaggtg
     5041 tgattaaaaa aacagcattc gggagagtgt cacgctctcc cgctctgtca gtattctgaa
     5101 ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg
     5161 gaggcgcgca gaatgcggat accacgagcg ccgacatcgc gtgcagcggt aatatcgtta
     5221 tcggaatcgc cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt
     5281 tgccctggtt tatcgcccgc aaagatcacc ggattcatgt tggtgacagg aatatgaaaa
     5341 ttatccgcca gcgtttttga aaccgtttct gttttcgtcg ggctacgacc agtcacaaag
     5401 aagatcgcgt caccgcggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga
     5461 atgctgaatt catcccagcc attgttcatt ttttcccaga acacaggatt tttcagataa
     5521 tcttcgcttt ctggtgagaa ggtttttttg ccgcgccaga agcccgggct ggaaaaaagt
     5581 accgtgtcat cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca
     5641 atttgtgcga ccgaaaccca atgaattggt gcctgttcag caagcctggc aacgttagtc
     5701 ccagggttaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac
     5761 aataagcaaa cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca
     5821 gtatgttgaa actttttatt gtgagatttg ttgcaaaaac catctgacca taacgccagc
     5881 tgccggggaa aggaaggatt atctgcggtt tttgtgatga aaagcaggat gattatgttt
     5941 aacccagtaa atacgttcct gtcttttttt g
//
LOCUS       DAZFAH010000237         5953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFAH010000237 DAZFAH010000000
VERSION     DAZFAH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.239"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..1238
                     /gene="ompC"
                     /locus_tag="ACHJWB_004314"
     CDS             135..1238
                     /gene="ompC"
                     /locus_tag="ACHJWB_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4315830.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1350..2405
                     /gene="apbE"
                     /locus_tag="ACHJWB_004315"
     CDS             1350..2405
                     /gene="apbE"
                     /locus_tag="ACHJWB_004315"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4315831.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2479..3543
                     /gene="ada"
                     /locus_tag="ACHJWB_004316"
     CDS             2479..3543
                     /gene="ada"
                     /locus_tag="ACHJWB_004316"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4315832.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3543..4193
                     /gene="alkB"
                     /locus_tag="ACHJWB_004317"
     CDS             3543..4193
                     /gene="alkB"
                     /locus_tag="ACHJWB_004317"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4315833.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4269..5912
                     /gene="yojI"
                     /locus_tag="ACHJWB_004318"
     CDS             4269..5912
                     /gene="yojI"
                     /locus_tag="ACHJWB_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4315834.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1402 a         1511 c         1624 g         1416 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaaaga tgtagatggc gaccagacct
      301 acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg
      421 tggcattcgc aggtctgaaa ttccaggatg tgggttcttt cgactacggt cgtaactacg
      481 gcgttgttta cgacgtaact tcctggaccg acgtactgcc agaattcggt ggtgacacct
      541 acggttctga caacttcatg cagcagcgtg gtaacggctt tgcgacctac cgtaacactg
      601 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggc aaaaacggta
      661 gcgtaagcgg cgaaggcatg accaacaatg gtcgcgatgc tctgcgtcag aacggcgacg
      721 gcgttggcgg ttctatcact tatgattacg aaggcttcgg tatcggtgct gcagtttcca
      781 gctccaaacg tactgatgct cagaacaccg ctgcttacat cggtaacggc gaccgtgctg
      841 aaacctacac cggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
      901 cccagaccta taacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
      961 ttgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtctgta gcatacctgc
     1021 agtctaaagg taaaaacctg ggtaccatcg ctggtcgtaa ctacgacgac gaagatatcc
     1081 tgaaatatgt tgatgttggt gctacctact acttcaacaa aaacatgtcc acctacgttg
     1141 actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg
     1201 ataacatcgt agctctgggt ctggtttacc agttctaatc tcgattgata tcgaacaaag
     1261 ggcctgcggg cccttttttc attgttttca gcgtacaaac tcattttttt ggtgtactct
     1321 tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc
     1381 tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc aaaacccacg
     1441 ctactgaagt taccgttctt gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc
     1501 cgggcattga tgccaaacgc agtgccgaac ttaaagaaaa gattcagacc cagctggacg
     1561 ccgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgact
     1621 cgcaaagttt gtcgccgtgg ccagtaagtg aagcgatggc cgaaatcgtc actacctcgc
     1681 tgcgcattgg cgcgaagacc gatggtgcga tggatattac cgtcgggccg ctggtgaatc
     1741 tgtggggctt tggcccggaa caacagccgg ttcaaattcc gagccaggaa cagatcgatg
     1801 cgatgaaagc caaaaccggc ttacagcacc tgacggtcat caatcagtcg catcagcaat
     1861 atctgcaaaa agacctgccg gatttatata tcgatctctc caccgtcggt gaaggttatg
     1921 cggcggatca cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg
     1981 tgggcggcgc gctgaacagc cgtggtatga acggtgaagg ccagccgtgg cgggtagcga
     2041 ttcaaaaacc aaccgataaa gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg
     2101 ggattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttccc
     2161 atgttatcga tccgcaaacc gggcgtccca tcgaacacaa tctggtatcc gtgacggtga
     2221 ttgctccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta ctcgggccgg
     2281 agaaagccaa agaggttgtt cgccgggaag ggctggcggt ttatatgatc accaaagaag
     2341 gcgatagctt taaaacctgg atgtcgccgc agtttaaaag cttccttatc agcgaaaaaa
     2401 attaaagcgc aagattgttg gtttttgcgt gatggtgacc gggcagacta aaggctatcc
     2461 ttaaccaggg agctgattat gaaaaaagcc acatgcttaa ctgacgatca acgctggcaa
     2521 tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg ttttcgccgt gcgtaccaca
     2581 ggcatctttt gccgtccgtc ttgccgcgcc agacatgctt tgcgggaaaa cgtctccttc
     2641 tacgcaaatg ccagcgaggc actcgccgct ggctttcgcc cctgcaaacg ttgtcagcca
     2701 gacaaagcca atgcccagca acatcggttg gataaaatca cccacgcctg tcgactgctg
     2761 gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc atgtggcgat gagtccgttc
     2821 catctgcatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc
     2881 tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt
     2941 cttaacgccg gattccccga cagcagcagt tactatcgca aagctgacga aacgctgggc
     3001 atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct
     3061 gattgtgagc tgggtcgttg cctggtggca gaaagcgagc gggggatttg cgcgatattg
     3121 ctgggcgatg atgacgcgac actaatcagc gagttgcagc agatgtttcc cgctgccgac
     3181 aacgcgcctg ccgatctgat gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat
     3241 caacgcgata cgccgctgac gttaccgctg gacattcgcg gcactgcttt tcagcaacaa
     3301 gtctggcagg cactgcgcac gataccttgc ggtgaaaccg tcagttatca gcaactggct
     3361 aacgccatcg gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag
     3421 ctggctatca taataccctg tcatcgggtg gtccgtggtg atggcacact ttccggttac
     3481 cgctggggcg tgtcgcgtaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg
     3541 taatgttgga tctgtttgcc gatgctgaac cgtggcaaga gccactggcg gctggagcgg
     3601 taattttgca ccgttttgct tttaacgctg cggagcaact aatccgcgat attaatgacg
     3661 ttgccagcca gtcgccgttt cgccagatgg tcacccccgg gggatatacc atgtcggtgg
     3721 cgatgaccaa ctgtgggcat ctgggctgga cgagccatcg gcaaggttat ctctattcgc
     3781 ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc acagagtttt cataatttat
     3841 gtcaacgtgc ggctacggcg gcgggctatc cagatttcca gccagatgct tgtcttatca
     3901 accgctacgc tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc
     3961 gcgcgccaat tgtttctgtt tctctgggct tacccgcgat ttttcaattt ggtggcctga
     4021 aacgtaatga tccgctcaaa cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg
     4081 gtgaatcgcg gctgttttat cacggtattc aaccgttgaa agcggggttt catccactca
     4141 ccaccgactg ccgctacaac ctgacattcc gtcaggcagg taaaaaagaa taaaaataag
     4201 aattattatt gctgtgcgcg cgaagatgtt taaactgcgg gctgtaattc attgtccggg
     4261 ttttctgcat ggaacttctt gtacttgtct ggcggcagta tcgctggcca tttatcagtg
     4321 tgatggcgct aagcctcgcc agtgcggcat taggcattgg cttaattgct tttatcaatc
     4381 agcgccttat cgaaacggcg gataccagtc tgctggtgtt gccggagttt cttggattat
     4441 tactgctgtt gatggcagtc actctcggat cgcaactggc gctcaccact ttggggcatc
     4501 acttcgttta ccgactgcgt agtgaattta tcaagcggat tctggatacc cacgtcgagc
     4561 gcattgaaca actcggtagc gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata
     4621 tcaccattgc ttttgtgcgt cttccggaac tggtgcaggg gatcattctc actatcggtt
     4681 ccgcggcgta tctgtggatg ctgtcgggca aaatgttgct ggtaacggct atctggatgg
     4741 cgatcaccat ctggggtggt tttgtactgg tggcgcgggt gtacaaacat atggcgaccc
     4801 tgcgtgaaac cgaagacaag ctgtacacgg attttcaaac cgtactcgaa gggcgcaaag
     4861 agctgactct gaaccgggaa cgcgccgagt atgtgtttaa caacctctac atccccgatg
     4921 cgcaagagta tcgccatcat attattcgcg cagacacctt ccatcttagc gccgtgaact
     4981 ggtcaaacat catgatgctg ggcgctatcg gcctggtgtt ctggatggcg aacagtctcg
     5041 gttgggctga taccaacgtt gctgcgacct attcgttgac gctgttattc ctgcgtacgc
     5101 cgctgctttc ggcggttggc gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca
     5161 agctgaacaa attcgcgctc gcgcctttca aagctgagtt tccgcgcccg caggcttttc
     5221 ccaactggca aacgctggag ctgcgtaacg tgacgtttag ctatcaggat aacgcgtttt
     5281 ccgttgggcc gattaacctc accattaaac gtggcgagct gctgtttctg attggtggca
     5341 acggtagtgg aaaatcgacg ctggcgatgt tgctgacggg cttataccag ccacaaagcg
     5401 gcgaaatctt gctggatggc aaacctgtca gtggcgaaca accggaagat tatcgcaaac
     5461 tgttttcggc agtgtttacc gatgtctggc tgtttgatca actgctgggg ccggagggta
     5521 aacccgctaa cccgcaactg gttgagaagt ggctggcgca gctgaaaatg gctcataagc
     5581 ttgagttaag caacgggcgt attgttaacc tgaagttatc aaaagggcag aaaaaacgcg
     5641 tggcgctgtt gctggcgctg gcagaagaac gcgatattat cctgctggat gaatgggcgg
     5701 cggatcagga tccacacttc cgtcgtgagt tttatcaggt gttgctgccg ctgatgcagg
     5761 agatgggtaa aactattttc gctatcagtc atgatgatca ttactttatc cacgccgacc
     5821 gcctgctgga aatgcgcaat gggcaactta gcgagctgac gggcgaagag cgcgatgccg
     5881 cttcgcgtga tgccgttgcc cggacggcat aatattacga cgctgacctg gcggactgct
     5941 gccgtcaggt caa
//
LOCUS       DAZFAH010000238         5930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFAH010000238 DAZFAH010000000
VERSION     DAZFAH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.240"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1025
                     /locus_tag="ACHJWB_004319"
     CDS             <1..1025
                     /locus_tag="ACHJWB_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4315835.1"
                     /translation="SSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAK
                     AEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGE
                     ERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDK
                     YYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEER
                     EGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFS
                     FINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVD
                     DYNSLR"
     gene            complement(1075..2823)
                     /locus_tag="ACHJWB_004320"
     CDS             complement(1075..2823)
                     /locus_tag="ACHJWB_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4315836.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEDKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2823..3893)
                     /locus_tag="ACHJWB_004321"
     CDS             complement(2823..3893)
                     /locus_tag="ACHJWB_004321"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4315837.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3883..5334)
                     /locus_tag="ACHJWB_004322"
     CDS             complement(3883..5334)
                     /locus_tag="ACHJWB_004322"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4315838.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIVSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5345..5791)
                     /locus_tag="ACHJWB_004323"
     CDS             complement(5345..5791)
                     /locus_tag="ACHJWB_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4315839.1"
                     /translation="MATLTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1539 a         1512 c         1511 g         1368 t
ORIGIN      
        1 tatcttccgc tattatagtc gccatcatgg ccctcggtct gacgggttgt gatgataaaa
       61 aagccgaaac agaaacgccc ccgcctgcca atagtcaacc tgccgcacca gctcctgaag
      121 ctcccgttgc aaaagcagaa gctaaacctg aaacacctgc gcaaccggtg gtcgatgaac
      181 aagcggtttt cgacgaaaaa atggatgtct atatcaagtg ctataacaag ttacagatcc
      241 ctgtacagag cagtctggcg cgttatgctg actggctgaa agattttaaa caggggccta
      301 ccggtgaaga gcgtactgtt tatggcatct acggcattag tgaatccaac ctcgctgagt
      361 gtgaaaaagg cgtaaaaagt gctgtggcgt taacgcctgc gctgcaacca attgatggcg
      421 tggcggtgag ttatatcgat gctgccgtgg cgttgggtaa taccattaac gaaatggata
      481 aatattacac ccaggaaaat tataaagacg atgcgtttgc gaaagggaaa acgctgcacc
      541 agacattctt aaaaaatctg gaagcctttg aacctgtagc agaatcttat catgcggcga
      601 ttcaggaaat taacgataag cgccagcttg cggaactgaa aaatattgaa gaaagagaag
      661 ggaaaacatt ccactactac tctctggcag tcatgatttc agcgaaacaa attaataacc
      721 tgatatcgca agagaagttt gatgttgatg cggcaatgaa gaaagtgtct gaactggaaa
      781 cgctagtggc gcaggccaaa gaagcggata aaggcggcat gaatttctcg tttattaatt
      841 cggcaggcca gtatcaactc gaggctaaaa aatacgttcg ccgcatcaga gataaagtcc
      901 cgtactctga ctgggataaa gagcaacttc aggacgcaaa ctcaagctgg atggtcgaag
      961 actctttccc gagagcatta cgcgagtaca acgaaatggt tgatgactat aatagcctgc
     1021 gttaaacgtt tttgatgata aatgcagtgt cggatgcggc gcatccgaca cagttcaccc
     1081 cttgtgaagt aactccataa atgcctcata tcctgttaac ccggcaataa tctccgccgg
     1141 atgctgacac aatatgcttg ccagatagct aaatattttt agctcatgac gcgcatcggc
     1201 ggcggcgcag gcgatcaaga catggttaat gacttcgtta ttcacctcaa ctggttgggc
     1261 gagggtaata aaaaatccac gaaaacgtcg ctcctgttcg ctccagcaat gtgggatggc
     1321 gaggcgatta acaatcaggt tttcaccttc gccttcgcgg gcgatgatgc gttgtgcttc
     1381 atcggcggta atatggtgtt gtgcgactaa ttgcgcacaa atttgccggg taatatgttg
     1441 ccagctttca ccgcgtacat tcgaataatg aaaacttccg ggggcagaga aaaaacgctc
     1501 cggctgttgg cgaataaatg ccgtcgccag aaaatgtttt atttgttcga taccggcagc
     1561 cgtaatgata ttttttacgg tgatgtaatt atttaccgcg tcatccagta aataatggct
     1621 gttgttaatg atcaataacg gctcaatctc ttcgcgaatg gcaacaagtt cgcttaagct
     1681 acgggcaata atcacccgac aatgtaaaac atcgcgctca atggcgagct gattaatagt
     1741 ggcaatcgcg ttctggtccg agagcaaaat gatcggctgg cgttcgtttt gatgccgttc
     1801 cagcgcacag gcaaaataca aaccgatcag atcgctgtcg aaaaggggaa tatcgagctg
     1861 ttcacgcagt agcgtaataa agtgcagact catatcaaag gccgcaggcc aggctgcttt
     1921 caggttattc atgctgcttt ggcggtgctc agcaatccac actggtgcgg caaggcaacg
     1981 taataaatgc ccggtaatgt tctcaaccag ctgcgtatca ataatattca atgacgcgat
     2041 gcccggcacc ctttgcagca accgctgcac attatcggca gaaattatct gcgcttgctg
     2101 atgctgtttc tctatcaaac ctgtcagggt tcttacagcg ttatcaccaa gaaacatgcc
     2161 gctttgttca acgatttgtt ttatctcgtc accgggatat tgcggccact catcggtcag
     2221 ttgattacgc agggcgtaaa tcgccagaat caggctggag agataatcac cctgcatgag
     2281 cggccagcgg tgttggttgt cgcaggccgt ggataaatgc tgaaggttgt ctcgtgttac
     2341 gcccgccagc ggaattaaaa acggatcttt gcgcagcaag ttcgccagca agataacgcg
     2401 cttatcttct gtctcatcaa tgaaatggcc caaaccgggg cggctggcca ggcaacaagc
     2461 gcgttcataa cgctgtttta accggggcag acgctctgcg acccacgttt ctggcaaatt
     2521 aagcgccgag gcgagttgcg cacggggagt aaaggtattc agtaataaca gtgccagcag
     2581 ccgatcgtcg ttatcgtgct tttgcagcaa ctgaaaaaag ctgcggcgct cgaagatttc
     2641 cagctgatag cccgcactac cactggcgga aatgcgggct ttgccgttaa gggtaaagtt
     2701 gagatagtca atatcacgca ggatggtcct gcctgaaacg ccagtctgtt gcgccagctc
     2761 accaggcgtg cgcggctgtt gctccagcag atcgacaatc tttaactggc gttcgtttag
     2821 cattaatcca cctgacggaa ctggctaaac gcgttgactt tctccgcagt taaggtcgtc
     2881 agaaaacccc gtatcagcgt gagcagagca tcgtaatcgg cttttgaaat cataccgctg
     2941 ttggcgtgca gataacgagt tggcagacac agcgcgacaa ccggacgccc gccgcccatc
     3001 acgttgtagc gcccgccatc cgtcgctccg gttttcatgg tggaaaattg cagcggtaaa
     3061 tcattctgcg tggcacagtt tttaaacgct gctaccagtt tctggttggg gaagtagcgc
     3121 ttgtcaaaca gcatcagccc cggcccctgg cccagtttca gcgggtattt aatgttatca
     3181 atgcccggaa catcacctgc gacagcggta tccagcacaa tcaccacatc cggtttaatg
     3241 tgttccgccg aggtttgtgc cccgcgtagc ccaacttctt cttccacgct gccgacgcca
     3301 tacagcgtaa tttcgggatt attcaccgtc tgcaatagtt cagccatcat tgcgcagccg
     3361 atgcggttat ccagcgcctt gccgaccact ttatcttcgc cccagcaggc aaaattggct
     3421 tccgggctaa taaaatcgcc catcgccaca ccgcgttttt ccacctcatc gcgactgttc
     3481 gcgccaatat caatgaacat ctcatcaaat gacagcggct gttgcttttg cttttccgtt
     3541 aacgcatgag gtgcgacgga accaatcaca ccagggattt taaatccctt gtgtgtgcgt
     3601 atggttaccc ggtggttgag cattgactga ttccaccagc cgccaatggt ggtaaagcgc
     3661 agaaaaccgc tctcgtcgat gtgggtgacc ataaagccga cttcatccat atgcccgaca
     3721 acggcaactt ttggaccttt attcccctta cgggcaacaa agctgcccag accatcaaag
     3781 gtgatctcat taacgcaagg ttccagcgtg tttatcagaa tgtcgcgaac ttcctgttca
     3841 tcgccgctga cggcgctggc ttcgcacaac tgttgcagta actcaatgtt catggacaag
     3901 ctcctgttgc gcggctttcg ctttgcggcg taaccacaat cctttcagga cgataatgaa
     3961 tgaaatgttc agcgcgaggc caatcgccag caccagataa aacgaaccca ccggcgacat
     4021 caggccgatc agcgggtcaa agatacccaa accaggtgcc agacgtttga ttccgaaagc
     4081 gatcaccagc acgccggtaa tgccgcccgc cagcgtattg gcggtaatca tcggcaacgg
     4141 agctgccaga gcgtaaggta tagccggttc ggttgcgact gtggcaccga ccacaatggc
     4201 actactggca gcctcttttt cctgtcgggt aaacagtttg ggcgcaatga acgtcgcaag
     4261 acccgccgca acgggcggca tcaacgcgac aacgccgacg atggcgtacc agtcataaat
     4321 gtgtttttcc agcagtgaga agcagaagaa ccaggcggtt ttattgatgg ggccgcccat
     4381 atcgaacgcc agcatcgcac cgaccagaaa cgctgccgca aacttcattg atggaggaat
     4441 ggtattcagg aagtggagca gcccgcccat gaggtcggac atcaccggcc cgataacgta
     4501 gtaagtgagc acgccgaaaa ccagcagagt aacgaacggg atcagcattg aaccgagcag
     4561 cggttgtaat gctttgccaa ggcgcacttt gcggaaccag aagacgaaat agccaatcgc
     4621 cagccccagc accaccgcgc cgaggaatcc cgcacccgac tgggtgccga gcagcgcttt
     4681 gtcgttcgcc agatagcaaa ccagaaacgc cggagcgaaa gccggtttat cggcaatcga
     4741 cgaggcgata taagcgccca tgatggggat cataaaggtg aagccgaggt aaccgatgga
     4801 ttcaactacc caggtaaacg acggcgcacc tttcgacata tcggtatacg gcaaaccgaa
     4861 ctgcaccagc atgttggcaa gcgccaccag aataccgccg ccgatgacaa acggcagcgc
     4921 cgcagagacg cccgccatca ggtggctcat cacgctgccg ctttgcactt cctgtttgcc
     4981 aagcttcacg ccgctatctg cagcaaaaag ctgcgagttt gttggtaatt cactgacaat
     5041 ctgatcgata tttttcagcg cctgggagat ggcaatctca taaaccttct tcccggcaaa
     5101 ccgcgcgcga tcgtcaccgc tcaggccacg cccggtagcg agaatgacgt aatcggcagc
     5161 ggcaatctct tcgctggata agcggttttc gacgccgctt gatccttgag tttcaacttt
     5221 aatggtatga ccgagagaac gggctttctg ttccagggct tccgccacca tgtaggtgtg
     5281 agcgatcccg gcggggcagt tggtgatcgc gacaatacgt aaggatgact ccatcgccac
     5341 ttccttatga gctgagggtt tgatttaaca aggcaagcac ctggtcggcg tcgccctgtt
     5401 gcagttgatg aataaaatcc tggtggataa ttttgcgaca cagtgtgccg atgattttga
     5461 cctgatcctc ttcgccgctt tgcggcacgc cgaggcagat ccagcaattg acgtcttcgc
     5521 catcgctggc tttccagtca atagcctgcg ctttgcgggc gaataatacg aacggttgtt
     5581 taacgcaggc gcttttaccg tgcggcacgg cgacaccgga accaaatcca gtagagtgca
     5641 ttttttcgcg cagcagcagg gtttgcagga actggtgtga gtcggtgata aaaccgtttt
     5701 gtaacgctat tgtcgccaac tgcttcagaa cggaataagc gccattgccc tgaatattca
     5761 ggtcaataca gcttgcagta agagttgcca ttaatcggac tccataaaat atattttaat
     5821 caattgtaat ggtggagcga ttaagtgatg agaggcgata tgtcggtaaa caatgacaat
     5881 ttgagacaga gtgaaaggtc agatttgcgg agtaatgcac ataatggtta
//
LOCUS       DAZFAH010000239         5899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFAH010000239 DAZFAH010000000
VERSION     DAZFAH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.241"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            198..1405
                     /locus_tag="ACHJWB_004324"
     CDS             join(198..519,519..1405)
                     /locus_tag="ACHJWB_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4315840.1"
                     /translation="MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYQEGSLTAVAAGEDVVPASELATAIKQINQLQRLLGKKTMEVEILKEAV
                     EYAQSPKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRPRRDEAAD
                     AEILSRILDIIGDMLGYGYRRVWAILRRQSRNEGLPFVNAKRVYRIMSENSLLLLHDK
                     PSRSQREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVMDCCDREAIDWAASTGG
                     YGKATVQDVMLGAIEKRFGDKGPERPIQWLTDNGSAYRAHETRQFTRELNLEPCTTTI
                     SSTQSNGMAERFVKMMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPR
                     EYRRQRVTLT"
     gene            1535..2763
                     /locus_tag="ACHJWB_004325"
     CDS             join(1535..1850,1850..2763)
                     /locus_tag="ACHJWB_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4315841.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGISLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMPGAVERRFGSELPTSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRASNGLSDNRCLEI"
     gene            complement(2967..3533)
                     /locus_tag="ACHJWB_004326"
     CDS             complement(2967..3533)
                     /locus_tag="ACHJWB_004326"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4315842.1"
                     /translation="MYTESNIKLSCNVLWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAMANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLDEDNSVTHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTAQAYIESHGAIERHLGCSDF"
     gene            complement(3561..4175)
                     /locus_tag="ACHJWB_004327"
     CDS             complement(3561..4175)
                     /locus_tag="ACHJWB_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4315843.1"
                     /translation="MITIETLYGECHEAHGLKIRNQVNNLFQKHPRITAIRVDLHYPP
                     VIDNGDTITCFPNLSPGEISRFRNSLNAKINADQHRKKQQGKRTYATSVCIIWAKEYT
                     QSFKCHYHVCLFFNKDAYYHLGDYERSDTLRGLITGAWYSALGLELEDHQGLVHFPAN
                     GRYILDINDPDFPEQYQRLLARLDYLTKLDTKASGQRNFGYSGF"
     gene            complement(4566..5126)
                     /locus_tag="ACHJWB_004328"
     CDS             complement(4566..5126)
                     /locus_tag="ACHJWB_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4315844.1"
                     /translation="MASFAYIDSEKWSKLNRLKSMIQRYFKEPLLQLTGELGRPDYTL
                     ADRKRLVRLLTQDMQRGDILITKSLTCFGNTPEKIQEVLSLSIRQGINICFFMSSELP
                     DGDDIPVVLTGMILPTDIRGKIPDISIRKIKRKPGRKEGSIHSKNIRQLKLQGFSQSQ
                     TARKLSISLSTVKRHWDKGIVDLTAE"
     gene            complement(5348..>5899)
                     /locus_tag="ACHJWB_004329"
     CDS             complement(5348..>5899)
                     /locus_tag="ACHJWB_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315845.1"
                     /translation="NEEPFVQRTKPEITKGEFFHSIYKSHIKYEYDVLDRKIFPHEST
                     RNAMGVAEKKGIKENATLMLEYYKVEKAICIYTNRKVSHTLNRAGGFYKTILIKTSVF
                     GDYFFDFCNSVCLPIDELIEYGTKETVRRHQIRSTGFCTFHIPIFYINNKAVIVPVLR
                     TEEVSQSSRTGGDVIIINPFEDE"
BASE COUNT         1697 a         1291 c         1344 g         1567 t
ORIGIN      
        1 attaatacgg gggatagttg acaataagac aattaatgac agatattagt gaataaatat
       61 aatcaggcgc ccaatgcttt atttattttg gggcgtctga ttaaaatgag ctggccccct
      121 gaaacaccag acagtagatg tatcttaaga taagaggtag gcttggatat atgtctaaca
      181 ctagttctaa ttttgaggtg gctggagttt tgttagggaa agaggtgcgt aaacgtaaaa
      241 cacctcagga aaaaatcgca attattcaac agactatgga gccaggtatg acggtgtccc
      301 acgtggcgcg tttgcatgga attcagccaa gtctgttgtt taagtggaag aaacaatacc
      361 aggaaggaag tctcactgct gtggctgccg gtgaagacgt tgttcctgct tctgaactgg
      421 ccactgcaat aaagcagatt aatcagcttc agcgtttact gggcaagaaa accatggaag
      481 ttgagatcct caaagaagcg gtggagtatg cgcaatcgcg aaaatggata gcgcatgtgc
      541 ccttgttacc ttcggacaag gaatagcccc ggtaagccgt gttctgggcg tgtcacgtgc
      601 gcagttaaca ttacggatga aggcatctga taacaagcca gataaacggc gtccgaggcg
      661 cgatgaggca gcggatgcag aaatcttatc gcgcatcctg gatatcattg gcgatatgct
      721 aggatacggg tatcgccgtg tctgggcgat actcaggcgg cagtcccgaa atgaaggact
      781 accgttcgtg aatgcaaagc gcgtttaccg gataatgagc gaaaatagcc tgttgttgtt
      841 acatgacaaa ccatcacgat cgcagcggga acataaaggc cgaatatcgg tgaaggaaag
      901 cgaccagcgt tggtgttccg atggctttga gttcggttgc gatgatggcg aaaaactgcg
      961 tgttacgttc gtcatggact gttgcgatcg tgaagccata gactgggcag caagcacggg
     1021 aggttatggc aaggctacag tgcaggatgt catgttagga gcgatagaaa aacgttttgg
     1081 cgataaagga ccggaacggc caatccagtg gctgacagat aacggttcag cgtacagagc
     1141 gcatgaaaca cggcagttca ccagagagtt gaatctggag ccctgcacaa cgacaatcag
     1201 cagcactcag agtaatggaa tggcagaaag gttcgtgaaa atgatgaagg aagactacat
     1261 cgcgttcatg ccgaaaccga atgtaagaac ggcattgcat aatcttgcag tggcgatcga
     1321 gcattacaat gaaaaccatc cgcacagtgc gttgggttat cgctctccgc gagaatatcg
     1381 acgtcagcgg gtaacgttaa cttaagatac aacccctgtc tggaaataag ggggcaagaa
     1441 cagtagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     1501 aatactagtt tttagactag tcattggaga gcagatgatt gatgtcttag gaccggagaa
     1561 acgcagacgg cgtaccacac aggaaaagat cgcaattgtt cagcagagct ttgaaccggg
     1621 gatgacggtc tccctcgttg cccggcaaca tggtgtggca gccagccagt tatttctctg
     1681 gcgtaagcaa taccaggaag gaagtcttac tgctgtcgcc gccggagaac aggttgttcc
     1741 tgcctctgaa cttgctgccg ccatgaagca gattaaagaa ctccagcgcc tgctcggcaa
     1801 gaaaacgatg gaaaatgaac tcctcaaaga agccgttgaa tatggacggg caaaaaagtg
     1861 gatagcgcac gcgcccttat tgcccgggga tggggaataa gcttagtcag ccgttgtctc
     1921 cgggtgtcgc gtgcgcagtt gcacgtcatt ctcagacgaa ccgatgactg gaaagatggt
     1981 cgccgcagcc gtcactcaga tgatacggat gtgcttctcc gtatacacca tgttatcgga
     2041 gagctgccca cgtatggtta tcgtcgggta tgggcgctgc ttcgcagaca ggccgaactt
     2101 gatggtatgc ctgcgatcaa tgccaaacgt gtttaccgga tcatgcgcca gaatgcgctg
     2161 ttgcttgagc gaaaacctgc tgtaccgcca tcgaaacggg cacatacagg cagagtggct
     2221 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
     2281 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
     2341 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgccagg agcggtggaa
     2401 cgccgcttcg gcagcgagct tccgacatct ccagtggagt ggctgacgga taatggttca
     2461 tgctaccggg ctaatgaaac acgccagttc gcccggatgt tgggacttga accgaagaac
     2521 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
     2581 cgtgattaca tcagtatcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
     2641 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactgggtta tcgctcgcca
     2701 cgggaatatc tacggcagcg ggccagtaat gggttaagtg ataacaggtg tctggaaata
     2761 taggggcaaa tccaatggcg cagatacgct tgcggatcga taccattcag acagcaggtc
     2821 cgttcactac cgtaatcact gccgaaaaac acattgattg cccctcaata atcctgccgt
     2881 caacgtcaaa attgctggtc gggttcgact tatttaattg tgtaaacagg gcctctcagc
     2941 gaaaggccct tatgcatcac ctgatgttaa aagtcactac atccaagatg acgttcaatg
     3001 gcaccatgcg attcaatgta agcttgggcc gtctgttcca gtacaccagg ctcagcgtta
     3061 tatgtgttag ctgcatcaaa taccaacgac agcacttcag gatacacaac cagatgtgta
     3121 actgagttat cttcgtccaa tacttttccc cacgcctgct caatcagatt tctgagaacc
     3181 accacctcac gactcttaca ccagacatcg ttattaagta gcagtaccat aagataagga
     3241 gtggtatcgt tagccatggc ctccctactc cagagataat ataaaggggt gggctcaaca
     3301 gatttatctt tacgtcgctt gcactgcaaa tattcagaaa ttagtctatg cagttcacca
     3361 gtaaaatcag ccatcagaga tggaattgcc ttattcatac ctgggcaagg tattaattta
     3421 aattgtaata atttaatttt aggatgtgta gctgcagccc gatacagagt tgcaaggaca
     3481 cacttttgcc agaggacgtt acaggaaagc ttaatgtttg attctgtata cataataaat
     3541 caccttacag ttacaatagg tcaaaaaccg ctgtagccaa agttacgctg acctgatgct
     3601 ttagtgtcga gcttcgtcag ataatccaga cgtgccaata agcgctgata ctgctcaggg
     3661 aaatcaggat cgttaatatc caggatgtaa cgtccattag cagggaaatg aactagtccc
     3721 tggtgatctt ccagttctaa ccctaaggca ctgtaccagg ctccggtaat taaccctctc
     3781 agggtgtcag agcgctcata gtctcccagg tggtaatagg cgtctttgtt aaaaaaaagg
     3841 cagacgtgat aatgacattt aaaggactgc gtatattctt tcgcccagat aatacaaacc
     3901 gacgttgcgt aggtgcgttt gccttgttgt tttttacggt gttgatctgc attaattttc
     3961 gcattcagcg agttacggaa acgagatatc tctccgggac tcaggttagg aaaacaggta
     4021 atggtatccc cgttgtcaat cacgggagga taatggagat ccacccgtat tgccgttatt
     4081 ctgggatgtt tctggaagag gttattcacc tggtttctta tttttaaccc gtgagcctca
     4141 tgacattcac cgtaaagagt ttcaatcgta atcatgttaa tattcctttt gctttcaggg
     4201 ataagctaat agttaaccta cccaatacac aacagaagca gatattaata ttcgtatcag
     4261 tagttcccag agggatatat aaccctctgg gcgtcaatta agaaaaacgc tactgcctat
     4321 tgttacatta gagaacagga tgatgttaca cagttacatt agaacgtttt aaaacattaa
     4381 acagagttat gatctacaca caactaatta tttggtatta gaataccatt agctctatta
     4441 gtacatcata tccatcatta cacatatacc agatactata catcacagat acaatatata
     4501 tattacctat ccaacgcaca tgcatttata actccattgc caatagctac aaggactata
     4561 aaatattact ctgcagttag atcaacaata cctttatccc agtgtctttt tacagtggat
     4621 aaactgatgc ttaattttct ggctgtttgt gactgactga acccctgcaa tttcaactgt
     4681 ctgatatttt tactatgaat actcccttct ttccttcctg gttttctttt aattttccgg
     4741 atactgatgt cgggaatttt tcctctaata tctgtaggta aaatcatccc ggtgagtacc
     4801 acaggaatat catcaccatc cggcaactct gatgacataa aaaagcaaat attaatacct
     4861 tgtctgatac ttaaagataa aacctcctgt attttttccg gcgtattacc aaaacaagtt
     4921 aacgatttag taatcagaat atcacccctt tgcatatcct gcgtcaggag tcgaactaac
     4981 cgttttctgt ctgccagagt ataatccggg cgacccaatt cgcctgttaa ctgcaatagt
     5041 ggttctttaa aatatctctg aatcatggat tttaagcgat taagttttga ccatttttca
     5101 gagtctatgt atgcaaaact cgccataatg cctcaaactg gtgtcatgaa ttaattattg
     5161 aacctgtttt ttcctgagtc gcattactct tttctaaggg agactctcca gatacatcgg
     5221 aaaagtactc gataaaagag ttggccactc ccgggtaatg attgctctct ttaacctcag
     5281 aaagcggttt ctgttcatca tttacaccac gatttctggg ttatgaataa atctacgcgg
     5341 gccataatca ttcatcttca aatgggttaa taataataac atctccacct gttctggaac
     5401 tttgtgaaac ttcctctgtt ctcagaacag gcactattac agccttatta ttgatataaa
     5461 aaatcggaat atgaaaagta caaaatcctg tactacgaat ctgatgtcgc ctcactgttt
     5521 ctttcgtacc atactcaatc aactcatcta tcggcaggca aactgaattg caaaagtcaa
     5581 aaaaataatc cccaaaaaca ctggttttaa tcagaatggt tttataaaac cctcctgcac
     5641 gattcagggt atgggaaact tttctgttcg tgtaaataca aattgccttc tcaactttat
     5701 aatattccag cattaaggtc gcattctctt taattccctt tttctctgca actcccatag
     5761 catttctggt gctttcatga ggaaatatct ttctatcgag aacatcatat tcatatttga
     5821 tatgcgattt ataaatgcta tgaaaaaact ctccttttgt tatttcaggc tttgtccgtt
     5881 gcacaaatgg ttcctcatt
//
LOCUS       DAZFAH010000240         5881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFAH010000240 DAZFAH010000000
VERSION     DAZFAH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.242"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..352
                     /gene="yjbE"
                     /locus_tag="ACHJWB_004330"
     CDS             110..352
                     /gene="yjbE"
                     /locus_tag="ACHJWB_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4315846.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            466..1104
                     /locus_tag="ACHJWB_004331"
     CDS             466..1104
                     /locus_tag="ACHJWB_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4315847.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1101..1838
                     /locus_tag="ACHJWB_004332"
     CDS             1101..1838
                     /locus_tag="ACHJWB_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4315848.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1838..3934
                     /locus_tag="ACHJWB_004333"
     CDS             1838..3934
                     /locus_tag="ACHJWB_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4315849.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3981..4259)
                     /locus_tag="ACHJWB_004334"
     CDS             complement(3981..4259)
                     /locus_tag="ACHJWB_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315850.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4275..4403
                     /locus_tag="ACHJWB_004335"
     CDS             4275..4403
                     /locus_tag="ACHJWB_004335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315851.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4529..4939
                     /gene="psiE"
                     /locus_tag="ACHJWB_004336"
     CDS             4529..4939
                     /gene="psiE"
                     /locus_tag="ACHJWB_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4315852.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4983..>5881)
                     /locus_tag="ACHJWB_004337"
     CDS             complement(4983..>5881)
                     /locus_tag="ACHJWB_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO4315853.1"
                     /translation="LNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAE
                     SILRIIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINV
                     VLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMA
                     IGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIA
                     VAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGK
                     TLEELEALWEPETKKTQQTATL"
BASE COUNT         1392 a         1533 c         1614 g         1342 t
ORIGIN      
        1 ccatctctaa ttccccgcgc cgtaattcgc atgctttagt tgtgtatact cgatcccgcc
       61 cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt
      121 tctgtatggc atttttgcca tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt
      181 acaggtgggc gaagcggcag ggtcggcagc aacgtcggtt tcggcgggga gttcctccgc
      241 gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg
      301 tgatggttct aataccggga ccaccacaac cacgaccacc agtacccagt aataaagtat
      361 gtatccccaa aataatttga gtcattgcat ctgtggcttg aagtatgaag ggatttacca
      421 taaccacact ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa gcgacctgca
      481 ctcattctta tctgcctgct attacaggcc tgttcagcca cgactaaaga gctgggcaat
      541 tcactgtggg acagtctgtt cggcacgcca ggcgtacagc tgacggacga tgatattcaa
      601 aatatgccct acgccagcca gtacatgcag cttaatggcg ggccgcaatt gtttgtggtg
      661 ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc cactcttgtc
      721 acacaacatg gtcgtctggt gaagactttg cttggcggcg acaacctgat tgaagtgaat
      781 aaccttgccg ccgacccgct gattaaaccc gcacaaattg ttgatggtgc aacatggacg
      841 cgcacgatgg gctggaccga gtaccagcag gtacgctacg ccaccgcacg ctcagtcttc
      901 aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg aaaccccggt tcgcgtgctg
      961 gacgaagaag tctccaccga ccaggcgcgc tggcataacc gctactggat cgacagcgaa
     1021 gggcaaattc gccagtcgga acagtatctc ggcgcggatt atttcccggt gaaaaccact
     1081 ctcatcaagg cggcaaaaca atgattaaac aaactattgt cgcattgatt ttgagtgtgg
     1141 gggcgtcatc ggtcttcgcg gcgggaaccg tcaaggtctt cagcaatggc agcagtgagg
     1201 ccaaaacgct gacgggcgca gagtatttaa tcgatctggt aggccagccg cggctggcaa
     1261 acagttggtg gcctggtgcg gtgattagcg aagagctggc aacggcggca gcattgcgtc
     1321 agcagcaggc gttgctgaca cggctggcag aacagggcgc agattccagc accgacgatg
     1381 ccgccgccat taacgcctta cgccagcaaa tccaggcatt gaaagtgaca ggcaggcaaa
     1441 aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg ccgttgcagg
     1501 gcaactacac gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc gggcttatca
     1561 gccatcctgg caatcagcca ttcactccag gtcgcgacgt ggcgagctat ctctctgacc
     1621 agagcctgct cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg gacggacgca
     1681 cgcaaaaagc gccggtggct tactggaaca agcgtcacgt agagccgatg cccggcagca
     1741 ttatttatgt aggcctcgca gactccgtct ggagtgagac gcctgatgcc cttaacgccg
     1801 acattcttca gactctgacg cagcggatac cccaataatg aaaaaaagac atctgcttag
     1861 cttactggcg ctgggcatta gcacagcttg ctacggcgaa acatatcctg cgcccattgg
     1921 tccgtcgcag tcggatttcg gtggcgtagg attattacaa actcccaccg cgcgtatggc
     1981 acgggaaggg gagttgagtc tgaactatcg cgataacgat cagtaccgtt attactcagc
     2041 ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgc tacaccgacg tgcgcacccg
     2101 gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg tataaagata aagccttcga
     2161 tctcaaactg cgtttgtggg aagagagtta ctggctgccg caagtggcgg ttggtgcgag
     2221 ggatatcggc ggtacggggc tgtttgatgc ggaatatctt gttgccagca aagcctgggg
     2281 gccgttcgat tttacgctcg gcctgggctg gggttatctg ggcaccagcg gtaatgtgaa
     2341 aaatccgctc tgttcagcca gtgataaata ttgctatcgc gataacagct acaaacaggc
     2401 gggatctatc gacggtagcc agatgttcca cggtcctgcc tcactgtttg gcggcgtgga
     2461 ataccagacg ccctggcaac cgctgcgcct gaaactggag tatgaaggca ataattatca
     2521 gcaggatttt gccgggaagc tggagcaaaa aagtaagttt aacgtcggtg cgatttatcg
     2581 cgttaccgat tgggccgacg ttaaccttag ctatgaacgt ggcaacacct ttatgtttgg
     2641 cgtcacgctg cgcaccaact ttaacgatct gcgcccgtct tacaacgata acgcccgccc
     2701 gcaatatcaa ccgcagccgc aggatgccat tttgcagcat tcggtggtgg cgaatcagtt
     2761 aacgctgttg aaatacaatg ccggacttgc cgatccgcag atccaggcga aaggcgatac
     2821 gctgtatgtc accggcgagc aggtgaaata tcgtgattcg cgcgaaggga tcatccgtgc
     2881 taatcggatc gtgatgaacg atctgccgga tgggatcaaa acgatccgca ttacggaaaa
     2941 tcgtcttaac atgccgcagg tgacgacgga aaccgatgtc gccagcctga aaaatcatct
     3001 cgccggagag ccgttgggcc acgaaacgaa gctggcgcaa aaacgcgtcg agccagtggt
     3061 tccgcagtcc accgagcagg gctggtatat cgacaaatca cgctttgatt tccatatcga
     3121 tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc agctgggcgt
     3181 gatgggaacg gcagatttgt ggctgacgga ccatctgctg accaccggca gcctgtttgc
     3241 aaatcttgcc aacaactacg acaagtttaa ctacaccaat ccgccgcagg actcgcactt
     3301 accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac gatgtctatg tgaataacct
     3361 gcaagccaac tacttccagc atctgggcaa cggcttctac ggtcaggtct acggtggtta
     3421 tctcgaaacc atgtttggcg gtgcgggggc agaagtgttg tatcgcccgc tggacagcaa
     3481 ctgggcgttt ggtctggatg ccaactacgt taaacagcgc gactggcgta gtgcaaaaga
     3541 tatgatgaaa ttcaccgact acagcgtgaa aaccggacat ctgaccgcgt actggacgcc
     3601 atctttcgct caggatgtgt tagtgaaagc gagcgtcggg cagtatctgg cgggcgataa
     3661 aggcggcacg ctggagatcg ccaaacgctt tgatagcggc gtggtggttg gcggctatgc
     3721 cacgatcacc aacgtttcga aagaggagta cggcgaaggg gacttcacca aaggcgtgta
     3781 tgtctcggta ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg cggcgattgg
     3841 ctggacgccg ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc agttgtatga
     3901 catgaccagc gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc cagttttcgc
     3961 aatcactggc accctcattc ttaatggcct gttgagccat caggaacgca gttcaccccc
     4021 ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt cgatatcggt
     4081 tgcagatctg gttgcctgag cgactgggcc atcattgggc tggagacatt gctttgcggt
     4141 aggtcggctt tatcagcagt accgagcgga ccagcataag caggaagaac agagactgat
     4201 aacatcaaag cagcaaaata aggcttcatt tttaccacct ttatcaggtt acgtttcatt
     4261 tgttccagag gaacatggtc gatttttcgc gcattgctgg tggctgggaa tcacctgaat
     4321 gggtgatttt tgaattaccg gctttggtgc ggtttgtctt gccggatgcg ccgccaggcg
     4381 cggcttatcc ggcctacggg taggtatatc cggctttggt ggaggcgcgc tccaaatcca
     4441 ggttgaacaa aacatacaca aaaaatatag atctccgtca catttttgcg ttatacagga
     4501 agctcgccac tgtgaaggag gtactgctat gacgtcactc tctcgtccgc gcgtggagtt
     4561 tatctccacc attttgcaga ccgtactcaa tcttggcctg ttgtgcctcg gcctgatttt
     4621 ggttgtcttc ctcggcaaag aaacggtgca tctggcagat gtgctgttcg cgccagaaca
     4681 aaccagcaaa tatgagctgg tagaaggact ggtggtttac tttctctatt tcgaatttat
     4741 cgcgctgatt gtgaaatact ttcagtccgg ttttcacttc ccgctacgtt actttgtcta
     4801 tattgggatc accgccattg tgcgcttgat catcgtcgat cataaatcgc cactggatgt
     4861 gttgatctac tcggctgcga tcctgttact ggtgatcact ctgtggctgt gtaattcgaa
     4921 acggctgaag cgggagtaaa aagacagcac gccgaactgg cgcggcgtgc tggacaggaa
     4981 gattacagcg tagcagtttg ttgtgttttc ttcgtttccg gttcccagag ggcttccagc
     5041 tcctcaaggg ttttaccttt ggtttccggg acaaatttcc acataaatag tgctgccaga
     5101 acgcccatac acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc caccagccag
     5161 gagtttttgt ccatcatcgg gaaggtccag gagacgaagt agttcgccag ccactgggcc
     5221 gccaccgcga ttgccagcgc tttaccacga atagcattcg ggaagatttc cgacagcagt
     5281 acccagcata ccggacccca ggacatggca aaggcagcaa catagaacag catcgacagt
     5341 agcgccacaa tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt
     5401 gccattccga gtgcgccgat aatttgcagt ggcttacgac caaatttatc caccgtcata
     5461 attgccagaa cggtgaaggt gaggttgata actccgacaa taatggtctg caacagcgcg
     5521 atatccgtgc tggcccccag cgttttgaac acttccggcg cgtagtacaa caccacattg
     5581 atgccgacaa attgctggaa gatggagagc ataacgccga ttacaatcac gcccacgcca
     5641 aacatcagca gacgaccacc ggttttgcgg ccatgatcca gggagtgttt aatttcctgt
     5701 actgcctgag ttgcaagcgt gttgcccata attatgcgca ggatactttc cgcctgttct
     5761 tgcttgccgc gtgacatcag ccagcgagga ctttctggca cggtatacag cagcattaag
     5821 aacagcagtg cagggataca ttccgaggca aacatataac gccagccgtc agtattcagc
     5881 c
//
LOCUS       DAZFAH010000241         5878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFAH010000241 DAZFAH010000000
VERSION     DAZFAH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.243"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..1069
                     /locus_tag="ACHJWB_004338"
     CDS             86..1069
                     /locus_tag="ACHJWB_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4315854.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKINAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1118..2602
                     /locus_tag="ACHJWB_004339"
     CDS             1118..2602
                     /locus_tag="ACHJWB_004339"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4315855.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFLNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2595..3566
                     /locus_tag="ACHJWB_004340"
     CDS             2595..3566
                     /locus_tag="ACHJWB_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4315856.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3563..4519
                     /locus_tag="ACHJWB_004341"
     CDS             3563..4519
                     /locus_tag="ACHJWB_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4315857.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            4606..5655
                     /gene="yahK"
                     /locus_tag="ACHJWB_004342"
     CDS             4606..5655
                     /gene="yahK"
                     /locus_tag="ACHJWB_004342"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4315858.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAVPHNLDDFTTLLKRDGTMTLVGAPATPHKA
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1355 a         1301 c         1636 g         1586 t
ORIGIN      
        1 ataaaaatat tcgaaaattc ttattctctc cagcgcgcta atttgggcga acacttcctg
       61 actaccctgc aatgaggctg aaatgatgaa taaacgtttt gttatcaata tggtgtcttc
      121 gctgttatta ggcgctgcgc taatttctgc gccgctccag gcagcggaaa aagtcgtggt
      181 gaatatttct aaagtcgatg gtatgccgtg gtttaatcgc atgggcgaag gtgtggttga
      241 ggcgggtaaa gcctttggtg ttaatgcctc acaggtgggg ccttcaagta ctgatgctcc
      301 gcaacaagtt aaaattattg aggatttaat cgcccgtaag attaatgcaa ttactatcgt
      361 gccaaacgat gccaacgtgc tggaaccggt attcaaaaaa gcgcgagatg ccggaattgt
      421 ggttctgacc aacgaatccc ccggtcagcc aagtgctaac tgggatattg aaatcattga
      481 taacgaaaaa tttgccgctg aatatgttga gcatatggcg aaacgcatgg ggggcaaggg
      541 cggctatgtg atttacgtgg gtagtttgac cgtgccgcag cataacttat gggctgactt
      601 gctggtgaaa taccagaaag agcattaccc ggatatgcat gaagttaccc gccgcatgcc
      661 ggttgccgag agtgtggatg actcacgtcg taccacactg gacctgatga aaacctatcc
      721 tgacttgaaa gcggttgtct cctttggttc aaacggtccg attggcgcgg gacgtgcggt
      781 gaaagagaaa cgggcgaaaa ataaagtcgc agtctatggc atgatgatcc cttcacaggc
      841 ggcatcactg attaaaagtg gtgatatcac cgaagggatt acttacgatc cggcctctgc
      901 aggttacgca ctcgcagccg tcgccagcac gttgctgaag ggtgaagaga tcaaacctgg
      961 attagaaatg cagaatctgg gtaaggccga cgtagatatg gataagcgaa ttatccgttt
     1021 ccataaggta ttactggtca acaaagacaa tatcgactca ctttattaat ccatttcttc
     1081 ggggcgaaag ccccgattct tattgccagg ggatgttatg gaaactttcc tttcccttcg
     1141 tcatatcaac aaaacgtttc atgccacgcg agcgctgcgc gatgtttctc tcgactttat
     1201 gtcgggtgaa gtgcactgcc ttgccgggca aaacggctgt ggtaaatcga cgttgattaa
     1261 aattatgtcc ggtgtttatc gcccggatga gggagcggaa attacgcttg gtgggaaaaa
     1321 ctggtcaaag ctgacacccg ccgcttcggt ggcgcagggg attcaggtga tttatcagga
     1381 cctctcttta tttcttaacc tgagtgtctg ggagaatatc gccgtgaatc actatcacca
     1441 cggtctgttt gttaaccgcc gtcgtctgcg tgaggtggcg caggcggcaa tgaccagtat
     1501 taacgtcaca ttgccactgg atacactcgt ttctgagctt tcaattgccc gctgtcagct
     1561 ggtggctatt tgccgtgcac tggcgcagga cgcacggttg atcgtgatgg atgaaccaac
     1621 cgcttcgctg acccatcagg aagtgcaagg gttattacag gtggtgcatc aattgcgtga
     1681 acgcgggatc tgcgtggtct tcgtcagtca tcgtctggaa gaagtgatgg aagtttccga
     1741 ccgtatttca gtgctgaaag atggtgagct ggtcgggact tttccggctg cagagatgac
     1801 cacaaaacaa ctcggtttcc tgatgaccgg ccaggagttt gaatatcagg tgcgagagtt
     1861 gtggcaggga aaatccagca ccccggtgct ggaggtgcgt aatttaagtc gtcatgggga
     1921 atatctaaat attaacttgc gggtggaagc gggcgaagtg gtatcgattg ttggcctgct
     1981 tggtgcgggg cgcacggaat tatgtctgag tctgtttggt atgacccgac cggatgctgg
     2041 cgagatcctc atcaacggcc agccggttac actgcatagt aaccaggacg ctatccgcca
     2101 cggtattggt tatgtttcgg aagatcgcat gtcgcgcggt ttggtgatgg cgcaatctat
     2161 tgaagacaac atcattagca ctgtttttca caaggtaaaa gatcgcttcg gctttctgag
     2221 tgaaactaaa gttcgtgacc tggtggacag gctaatcaag gcattgacca tcaaagtctc
     2281 tgatcctcat cttccggtga atacgctttc tggtggtaac gcgcagcgag tatcgatcgc
     2341 caaatggctg gcgattgggc cacgactgtt gattctcgat agcccgacag tcggtgttga
     2401 tatcgccaac aaagcaggaa tatacggcat tatcagcgat ctggcggctc atggcattgc
     2461 ggtattgatg atctgcgatg aaattgaaga agcgtggtat caaagccacc ggattttggt
     2521 aatgcaaaaa ggtcagatca cccatagctt cctgcctgac agcagttctc aggccaggat
     2581 tgcggaggtg gtaaatggct gaactgaaaa aacgccatga attctggctg gcattactga
     2641 ttgtggtgct gtttgttggc cttgcctggc gcagcgacga gtttctgaca ttcggtaatt
     2701 tgtacgatct cgccaataac tatgccatgt tgactattct cgcctgtggt ttgtttgtgg
     2761 tgttgatttc cggtggaatt gatatttcgt ttccagcaat gaccatcatt gcgcaatacg
     2821 gcatggtgct gttgctgcaa aaaattggtg gcaacttcgc tgtcgcgttt gcactggcgg
     2881 gctgcatcgg cattttactt ggcttaatta acgccttact ggttaatcgc ctacgggtgc
     2941 cttctatcat catcactatc tcgacgctga atattttcta tggcctgctg ttatggttga
     3001 gtaaaggtgt gtggctgtac gactttccgc cgtggtttga gcagggggtt atgttgttca
     3061 agtacaccga tgctgatggc tatgactatg gccttggtct gccgctgatc gccatgatta
     3121 cggtggtgct gctaacagcg tttatcatga atttcaccag tgtagggcgc aaaatttatg
     3181 cccttggcgg gaaccgcgaa tcagccagtc gcatcggctt tagcgtgctg aaactgcaac
     3241 ttttcgtcta tggctatatg ggattgatgt ctggcgctgc gggtgtagtg cagtcgtgga
     3301 cggtgatgac tgtcgccccc gattctcttc tgggttatga gctgacagta ctggctgcgg
     3361 tggtgcttgg cggcactagt ttgctcggcg ggcgcggcac gttaaccggt actttgctcg
     3421 gcgtggtgtt gttggcagtg atgcaaaacg ggctaaattt attgggagtc tcgtcttact
     3481 ggcaaacatt gatcaccggc atcatcatcg ttgccagcat tagtgccacg gcgtggagtc
     3541 agcatcagaa ccggagtctg ctatgaaaaa atcgtggcgt aataacgttg agttttactt
     3601 gatcggcctg ctggtgctaa ctgttgccgc gttcagcatt actatgccgg agattttctg
     3661 gtcgataagt aatttccagt ccgtcgcttc gcaaatgccc gtgctgggca ttctggcgtt
     3721 ggctatggcg gtgaccatgc tttgcggtgg cattaacttg tcaattatag ccactgctaa
     3781 cgcctgttca ctggtgatgg cgtgggtggc gactcagtat ccaccgggta ttgctacagt
     3841 tgtcgctacg ttactggcgg gcgctggcgc ggcggtgata atcggtttat gtaacggcgt
     3901 attgattgct ggtattcgag tgtcgccaat tcttgcaacg cttgggatga tgacgttgct
     3961 caaagggata aatattctgg tgacaggtgg tagcgccatt gccaattatc cctcatgggt
     4021 gctgtggttg aatcatgcgc aatggttcgg tattccgcta ccgatgtggt tgtttgccgc
     4081 agtggcactg gggctatgga tattgctgga gaaaacgccg ctcggtaaag ccatttacct
     4141 gattgggtct aacgaacgag caacacttta ctccggcatt aacactcgtc gggtgctgat
     4201 ttgggtttat gtcatttccg ccttgctttg tgcggtagcg gcatttttga tgatgtcgaa
     4261 actcaactcg gctaaggcct cttatggcga atcgtacttg ctggtgtcaa tcctcgcggc
     4321 agtactcggt ggggttaatc cggacggcgg cagcgggcgg ataattggta tggtgctggc
     4381 gctgtttctt ctgcaaatta ttgaaagcgg cttcaatatt ctcggtataa gcccctacct
     4441 gacgatggcc ttatggggaa cgttgctgct ctgtttcatt caggttcgcg gcatgttggg
     4501 gctggatcgg gtggtttaaa tattggctat attcaatgga cgcgttttgc cgcgatgaca
     4561 tatcaggcat cgccaaatac acatagctaa tcaggagtaa acacaatgaa gatcaaagct
     4621 gttggtgcat attccgctaa acaaccactt gaaccgatgg atatcacacg gcgtgaaccg
     4681 ggaccgaatg atgtcaaaat cgaaatcgct tactgtggcg tctgccattc cgatctccac
     4741 caggtccgtt ccgagtgggc ggggacggtt tacccctgcg taccgggtca tgaaattgtg
     4801 gggcgtgtgg tagccgttgg tgatcaggta gaaaaacatg cgccgggcga tctggtcggt
     4861 gtcggctgca ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg gctggaaaac
     4921 tactgtgatc acatgaccgg cacctataac tcgccgacgc cggacgaacc gggccatact
     4981 ctgggcggct actcacaaca gatcgtcgtt catgagcgat atgttctgcg tattcgtcac
     5041 ccgcaagagc agctggcggc ggtggctcct ttgttgtgtg cagggatcac cacgtattcg
     5101 ccgctacgtc actggcaggc cgggccgggt aaaaaagtgg gcgtggtcgg catcggcggt
     5161 ctgggacata tggggatcaa gctggcccac gcgatggggg cgcatgtggt ggcatttacc
     5221 acttctgagg caaaacgcga agcggcaaaa gccctggggg ccgatgaagt tgttaactca
     5281 cgcaatgccg atgagatggc ggctcatgtg aagagtttcg atttcatttt gaatacagta
     5341 gctgtgccac ataatctcga cgattttacc accttgctga agcgtgatgg caccatgacg
     5401 ctggttggtg cgcctgcgac accgcataaa gcgccggaag ttttcaacct gatcatgaaa
     5461 cgccgtgcga tagccggttc tatgattggc ggcattccag aaactcagga gatgctcgat
     5521 ttttgcgccg aacatggcat cgtggctgat atagagatga ttcgggccga tcaaattaat
     5581 gaagcctatg agcgaatgct gcgtggtgat gtgaaatatc gttttgttat cgataatcgc
     5641 acactaacag actgaaaaaa ttaataaata cgctgtggtt taacatatta acttcgctct
     5701 ccacttaact ttttagttaa ggagagcgaa ataatatcaa agtagcagta aaacctataa
     5761 cgtaaattca aattgttaaa ttaactccct ccagtacact atacttcaca cgttagttat
     5821 gagcgatttc tgatagtgcc tggtttaatc agcgctttat tatctgcgac gtttattt
//
LOCUS       DAZFAH010000242         5859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFAH010000242 DAZFAH010000000
VERSION     DAZFAH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.244"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..316
                     /locus_tag="ACHJWB_004343"
     CDS             <1..316
                     /locus_tag="ACHJWB_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4315859.1"
                     /translation="RADETIRGYMGTSATMTSGEQSGAVIRGVFDDPENISYAGQGVR
                     VEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRVTPDDGGSCHLWLGRGVPPAVNR
                     RR"
     gene            328..906
                     /locus_tag="ACHJWB_004344"
     CDS             328..906
                     /locus_tag="ACHJWB_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4315860.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            903..1298
                     /locus_tag="ACHJWB_004345"
     CDS             903..1298
                     /locus_tag="ACHJWB_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4315861.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1306..2046
                     /locus_tag="ACHJWB_004346"
     CDS             1306..2046
                     /locus_tag="ACHJWB_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4315862.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            2062..2484
                     /locus_tag="ACHJWB_004347"
     CDS             2062..2484
                     /locus_tag="ACHJWB_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4315863.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            2466..2900
                     /locus_tag="ACHJWB_004348"
     CDS             2466..2900
                     /locus_tag="ACHJWB_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4315864.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2893..5454
                     /locus_tag="ACHJWB_004349"
     CDS             2893..5454
                     /locus_tag="ACHJWB_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4315865.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            5451..5780
                     /locus_tag="ACHJWB_004350"
     CDS             5451..5780
                     /locus_tag="ACHJWB_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4315866.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5780..>5859
                     /locus_tag="ACHJWB_004351"
     CDS             5780..>5859
                     /locus_tag="ACHJWB_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4315867.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEI"
BASE COUNT         1368 a         1442 c         1907 g         1142 t
ORIGIN      
        1 ccgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
       61 gcagtccggc gcagtaatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
      121 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
      181 gcagctgcgg cgtggagaca cgctgaccat cggtgaggaa aacttctggg tagatcgggt
      241 tacgccggat gatggcggaa gctgtcatct ctggcttggg cggggggtac cgcctgccgt
      301 taaccgtcgc cgctgaaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
      361 aacctcagcc gtatcagcaa aacggcggtg cctggtgccg ccgcaatggc cattaaccgc
      421 gttgcttcat ccgcgatatc gcagtcggcg tcacaggttg cccgtgagac aaaggtacgc
      481 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
      541 agaatcaaag ttaaccgggg ggatttgccc gtaatcaagc tggggaatgc gcgggttgtc
      601 ctgtcccgac gcaggcgtcg taaaaagggg cagcgttcag ccctgaaagg tggcggcagc
      661 gtgcttgtgg tgggaaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggg
      721 cggtggcatg tcatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
      781 atcccgatgg cggtgccgct taccacggcg tttaaacaga atattgaacg gatacggcgt
      841 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgagaat ggtaataaag
      901 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
      961 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     1021 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     1081 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     1141 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gatttgatca
     1201 ccagtatggt ggccagcggc tatgactacc ggcgcgacga tgatgcgggc ctgtggagtt
     1261 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     1321 cctacaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     1381 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     1441 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgca
     1501 gactggactg cgaccgggca ggggcagaaa tccgccggag ataccagctt cacgctggcg
     1561 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     1621 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     1681 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa agtcaccaac
     1741 gtgggacgtc cgtcaatggc agaagatcgc agcacggtaa cagcggcaac cggcatgacc
     1801 gtgacgcctg ccagcacttc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     1861 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     1921 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     1981 attccggttg tatccggtaa tggtgaactt gctgcggttg cagaaattac cgtcaccgcc
     2041 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggtgtg
     2101 accgtcacgc tttctgaact gtcagccctg cagcgtattg agcatctcgc cctgatgaaa
     2161 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttactgtgga agacgccatc
     2221 agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     2281 ctgccgtcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     2341 acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     2401 gtggtgaata atgcccctga acagacagag gacgccgggc ccgcagagcc tgtttctgcg
     2461 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgtgag atggggcgac
     2521 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     2581 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     2641 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     2701 tgaaccggcg cgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     2761 ggcttgccgg aggtgtccgc tttggcccgg acgggaatga agttatccct acttccccgg
     2821 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     2881 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttgag tctggatgcg
     2941 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     3001 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     3061 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     3121 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     3181 ggtggtcagg ttaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     3241 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     3301 tatgcctggt atcagggcaa ctcaaccttg tccgatttca acaaaacgct ggtcctttcc
     3361 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     3421 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggagta
     3481 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     3541 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     3601 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     3661 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgcg
     3721 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggaaacc
     3781 tgggcagaca ggacagcacg ggcattcaaa tccatgtggg atgcggtgct ggatattggt
     3841 cgtcctgata ccgcgcagga gatgctgatt aaggcagagg ccgcgtttaa gaaagcggac
     3901 gatatctgga atctgcgcaa ggatgattat tttgttaacg atgaagcgcg ggcgcgttac
     3961 tgggatgatc gtgaaaaggc ccgtcttgcg cttgaagccg cccgaaagaa ggctgagcag
     4021 cagagtcaac aggacaaaaa tgcgcagcag cagagcgata ccgaagcgtc acggctgaaa
     4081 tataccgaag aggcgcagaa ggcttacgaa cgcctgcaga cgccgctgga gaaatatacc
     4141 gcccgtcagg aagaactgaa taaggcactg aaagacggga aaatcctgca ggcagattac
     4201 aacacgctga tggcggcggc gaaaaaggac tatgaagcga cgctgaaaaa gccgaaacag
     4261 tccggcgtga aggtgtctgc gggcgatcgt caggaagaca gtgctcatgc tgccctgctg
     4321 acgcttcagg cagaactccg gatgctggag aagcatgccg gagcgaatga gaaaatcagc
     4381 cagcagcgcc gggatttgtg gaaggcagaa agtcagttcg cggtactgga ggaggcggca
     4441 caacgtcgcc agctgtccgc acaggagaaa tccctgctgg cgcataaaga cgagacgctg
     4501 gagtacaaac gccagctggc tgtacttggc gacaaggtca cctatcagga gcacctgaac
     4561 gcgctggcac agcaggcgga taaattcgca cagcaacaac gggcaaaacg ggccgccatt
     4621 gatgcaaaaa accgggggct gactgaccgg caggcagcgc gggaagccac ggaacagcgc
     4681 ctgaaggaac agtatggcga taatcctctg gcgctgaata acgtcatgtc agagcagaaa
     4741 aagacctggg cagctgaaga ccagcttcgc gggagctgga tggcaggcct caggtcaggc
     4801 tggagtgagt gggaagagag cgccacggac agtatgtcgc aggtaaaaag tgcagccacg
     4861 cagacctttg atggtattgc acagaatatg gcggcgatgc tgaccggcag tgagcagaac
     4921 tggcgcagct tcacccgttc cgtgctgtcc atgatgacag aaattctgct taagcaggca
     4981 atggtgggga ttgtcgggag tatcggcagc gctattggcg gggctgttgg tggcggcgca
     5041 tccgcgtcag gcggtacagc cattcaggcc gctgcggcga aattccattt tgcaaccgga
     5101 ggatttacgg gaaccggcgg caaatatgag ccagcgggga ttgttcaccg tggtgaattt
     5161 gtcttcacaa aggaggcaac cagccggatt ggtgtcggca acctgtaccg cctgatgcgg
     5221 ggctatgcgg aaggtggtta tgtgggcggt gccggaagtc cggcgcagat gcggcgggct
     5281 gaaggcatta attttaatca gaacaatcac gtggtgattc agaacgacgg tacgaatggt
     5341 ctgccaggtc cacagatgat gaaggcagtg tatgacatgg cccgcaaggg tgcccgtgat
     5401 gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg atgaagacct
     5461 tccgctggaa agtgaaaccc ggtatggatg tggcttcggc cccttctgta agaaaggtgc
     5521 gctttggtga tggctattct cagcgagcgc ctgccgggct gaatgccaac ctgaaaacgt
     5581 acagcgtgac gctttctgtc ccccgtgagg aggccacggt actggagtcg tttctggaag
     5641 agcacggggg ctggaaagcc tttctgtgga cgccgcctta tgagtggcgg cagataaagg
     5701 tgacctgcgc aaaatggtcg tcgcgggtca gtatgctgcg tgttgagttc agcgcagagt
     5761 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     5821 tgcggagcag tcggccagcg tggtgctctg ggaaatcga
//
LOCUS       DAZFAH010000243         5849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFAH010000243 DAZFAH010000000
VERSION     DAZFAH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.245"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..411)
                     /locus_tag="ACHJWB_004352"
     CDS             complement(<1..411)
                     /locus_tag="ACHJWB_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4315868.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPL"
     gene            729..1370
                     /gene="udk"
                     /locus_tag="ACHJWB_004353"
     CDS             729..1370
                     /gene="udk"
                     /locus_tag="ACHJWB_004353"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4315869.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERIKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1462..2043
                     /gene="dcd"
                     /locus_tag="ACHJWB_004354"
     CDS             1462..2043
                     /gene="dcd"
                     /locus_tag="ACHJWB_004354"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4315870.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2065..3918
                     /gene="asmA"
                     /locus_tag="ACHJWB_004355"
     CDS             2065..3918
                     /gene="asmA"
                     /locus_tag="ACHJWB_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4315871.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPACQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4192..5775)
                     /gene="yegH"
                     /locus_tag="ACHJWB_004356"
     CDS             complement(4192..5775)
                     /gene="yegH"
                     /locus_tag="ACHJWB_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4315872.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         1449 a         1491 c         1578 g         1331 t
ORIGIN      
        1 cagaggcgga acaatggcgc tgccgagtgc cagacgcagc cagtcagcca gattttgcaa
       61 ggggttatac cacggcagca atttacgtag cagcactgcc ccgaccacgg cttcaacaat
      121 attgatggtc gtccaggtca tgttcagcga gctggtggaa aaaagcagga tggatgcggc
      181 gatatttccc agcgagcagc tcagcgcaat tcccggccac atgcgcccgg catggcgata
      241 aaacgccacc atcatgatgg acgtcgggaa ccacagtggg gcgagttggg tgccaaactg
      301 cgaaagctca agcgagaaaa gggtaaagat aaacgagact aaacctaaac tgaccaggtg
      361 aagcagcggg tggggcaggg caattaatac atgctgtgat tgtttgctca ttaccgcttt
      421 atccgcaaga ccaggtcgcc agtattcgtt tctgtgttta tgctagtaca aacgttttac
      481 attttatatg cgaattgctc ataaagtgac gtaaaggagg ataatttgcg caactgcgta
      541 taacattttt taccttgtat aaaactgatc aacgtaattt gccaccggga attcttcgtc
      601 aacaattaac tctgagcgcg cggcaacgct attcgactgg tatcaggcgg atgaaatccc
      661 tataattgcc gcgtttggcg cttcgtcgcc cccttcctaa catccaggtt aatcaggtcg
      721 ctaaatatat gactgatcag tctcatcagt gcgtcattat cggtatcgct ggcgcatcgg
      781 cttccggcaa gagtcttatt gccagtaccc tttatcgtga attacgtgag caagtcggtg
      841 atgaacacat cggcgtaatt cccgaagact gctattacaa agatcaaagc catctgtcga
      901 tggaagaacg cattaagacc aactacgacc atcctagcgc gatggatcac agcctgttgc
      961 ttgagcattt acaagcgttg aaacgcggct cggcaattga cctgccggtt tacagctatg
     1021 ttgaacatac gcgtatgaaa gaaacggtga cggttgagcc gaagaaagtc atcattctcg
     1081 aaggcatttt gttgctgacg gatgcgcgtt tgcgtgacga acttaacttc tccattttcg
     1141 ttgatacccc gcttgatatc tgcctgatgc gccgcatcaa gcgtgacgtt aacgagcgtg
     1201 ggcgttcaat ggattccgtg atggcgcaat atcaaaaaac cgtgcgcccg atgttcctgc
     1261 aattcattga gccttctaaa caatatgcgg acattatcgt gccgcgcggc gggaaaaacc
     1321 gcattgcgat cgatatattg aaagcgaaaa taagtcagtt ctttgaataa gcttgataaa
     1381 ttgtgtaccg ttcagtgata acctggtatg cccttgacgt aattggcgtt aagggagtga
     1441 tgcgcgaaag gagaaaatgc catgcgtctg tgtgaccgag atattgaagc ctggcttgat
     1501 gaaggccgtt tgtcgatcaa cccacgtccg ccagtggagc gtattaacgg cgcgacggtg
     1561 gatgtacgcc tgggcaataa atttcgtacc ttccgtggtc acacggcagc gtttatcgat
     1621 ctgagcggtc ccaaagatga agtgagcgcc gcgcttgacc gcgtgatgag cgatgagatc
     1681 gttctcgacg agggcgaggc gttctatctt cacccaggag agctggcgct ggcggtgacg
     1741 ctggagtcgg tgacgctgcc agccgatctg gtgggctggc tggacgggcg ttcctctttg
     1801 gcgcgtctgg ggcttatggt gcacgtcacc gcgcaccgca tcgatccggg ctggtctggc
     1861 tgcattgtgc tggagttcta caactccggt aagctgccgc tggcgctgcg tccgggcatg
     1921 ttaattggtg cgctgagctt tgagccgctt tccggcccgg cggcgcgacc ttacaaccgc
     1981 cgtgaagatg cgaaatatcg caaccagcag ggcgcggtag ccagccgaat cgataaagac
     2041 taattcaggc ccattgagga taccatgaga cgatttctga cgacgctgat gatactcctg
     2101 gtcgtgctgg tggccgggtt atctgcgtta gtgttgctgg tgaatccgaa tgatttccgc
     2161 gactatatgg tcaagcaagt tgctgcacgt agcggttatc aattgcagct cgacgggcca
     2221 ctgcgttggc acgtctggcc gcagcttagt atcctctccg ggcgaatgtc tctcaccgcc
     2281 cagggcgcaa gccagccact ggttcgcgcc gacaacatgc gtctggacgt ggcgctttta
     2341 ccactactga gtcatcaact gagcgttaag caggtgatgc taaaaggggc agtgatccaa
     2401 ctgacgccgc agacggaagc ggtgcgcagt gaagacgctc cggttgcacc gcgcgacaat
     2461 accttgccgg atctgtcaga cgatcgcgga tggtcgtttg atatatccag tcttaaggtg
     2521 gcggacagcg tgctggtgtt ccagcatgaa gatgacgagc aggtgacaat ccgcaatatc
     2581 cgcctgcaaa tggaacaaga tccccaacat cgtggctcat ttgagttctc cgggcgggtt
     2641 aatcgcgatc agcgcgatct cacaatatcc cttaacggta cggtggatgc ttctgattat
     2701 ccgcatgatt taacggcggc tattgaacaa attaactggc agttgcaggg tgccgattta
     2761 ccaaaacaag gtattcaggg gcaggggagt ttccaggccc agtggcagga gtcacataaa
     2821 cgcctttcat ttaaccaaat cagcttgacc gccaatgaca gtacgctgag cgggcaagca
     2881 caggtcacgc tgacagagaa accggaatgg cagctgagac tgcaattccc gcaactgaat
     2941 cttgacaacc tcatcccgct taatgaaacc gcgaatggtg aaaacggtgc cgcgcagcag
     3001 gggcagagcc aatcaacgtt gccgcgcccg gtcatttctt cgcgtattga tgaaccggcc
     3061 tgtcagggac tgcaaggctt tacggctgat attttgttgc aggccagtaa cgtgcgctgg
     3121 cgcggaatga attttacaga tgttgccacg caaatgacca acaagtcggg tttgctggaa
     3181 attactcaac tgcagggcaa acttaacggt ggacaggttt cactgccggg cacgctggac
     3241 gcgacatcaa taaatccgcg gataaacttc cagccacggc tggaaaacgt tgagattggt
     3301 actattctga aggcgtttaa ctatccgatt tcgttgaccg gaaaaatgtc actggctggt
     3361 gatttctccg gtgctgacat agatgccgac gcattccgcc acaactggca aggacaggca
     3421 catgtcgaaa tgaccgacac gcgcatggaa gggatgaact tccagcagat gattcagcaa
     3481 gcggtagagc gtaatggcgg tgatgtgaag gccgctgaaa actttgataa cgtgacgcgg
     3541 cttgaccgct ttaccaccga tttgacgttg aaggatggcg tcgtgacgtt aaacgacatg
     3601 caaggtcaat cgcctgtgct ggcgctgaca ggggaaggca tgttgaatct ggcagatcaa
     3661 acctgcgata cccagtttga tattcgggtc gtgggtggct ggaacgggga aagtaaactg
     3721 attgatttcc tgaaagaaac gccagtaccg ctgcgggttt atggcaactg gcagcaactc
     3781 aattacagcc tgcaagtgga tcagttactg cgcaaacatc tacaggacga agcgaaacgt
     3841 cgcctgaatg actgggccga gcggaataaa gattcccgca atggcaagga tgtgaagaag
     3901 ttgctggaga agatgtaggt taccaggtga ccagtcctgt aggcctgata agacgcgtaa
     3961 agcgtcgtat caggcgttcg tggcggcatc attatattct gaatatgtaa gatcgaaggc
     4021 tgttccgttc acttgatgtt cctcgctact atggaacgct caagccggtg aacgtgccag
     4081 ggtggctcaa tcgccaccac cctggcaacc cgggctcccg gcggtaaatc gccgctggcg
     4141 cggttccctc atcttattcc tttcggctat tgggtaaaat ggccgatacc atcacacctc
     4201 gtactccatc tcgccatctt tacgcagtgg aataatctgc actttctgca cgcgatggct
     4261 ttccacctgc aacgttttaa gcaaataatc ccccacctga acttcttcgc caggttttgg
     4321 aatacgctgt aaatattcca tcaacagccc ggcaatggtg tgatattcac gtttttcatc
     4381 cagcggcagc ggcacatatt gcaccagatc ctccagcggc atatgaccat tcgccgtcca
     4441 ggaaccgtcc gcattcttct gaatatcatg gcgggcgtcg atctcttcca cttcgttcgg
     4501 taagttaccg gcaatggttt cagtgacgtc acttaatgtc acaatccctt ccaccgagcc
     4561 aaactcatcc accacaaaag caaagtgcgt gcgggcatta cggaactgct ccagggcagg
     4621 taacaacggc aaggtttccg ggaacaccag cggctggcga atcaacaccc gcaggttgag
     4681 cggttcgccg cgcagagact gttgtagcag gtcgataacg tgaacaacac cgagcaaatc
     4741 ttctgcgtca tcaccgtcgg taaccaccag ccgcgtatgc tgatttcgct ccagtaactg
     4801 gcggatctcg tcttccggcg cgttgagatc gatatgctca atatcgtggc gcgacgtcat
     4861 aatgctgctg acggtacgct ggttaagatt aagtacccgc tcaatcatcc gccgttcctg
     4921 cggattaaag atctgctggt taccatgatc caccagcatg gacgcggttt cggcgtccag
     4981 ttccgcatct tctttttgcc cgctaagcag gcgcattacc gcttcggtag tccgctggcg
     5041 cagcgtctgg ttagcggaaa gaaaacgtcg ccggttaaag atagccaact gattgagcgc
     5101 ctcgatcatc accgagaaac caatggcagc gtacaggtag cctttcggaa tgacgaagcc
     5161 gaaaccttct gccaccaggc taaagccaat catcaacagg aagcttaagc agagaataac
     5221 gatcgtcggg tgactgttaa caaattgcgt taacggcttg ctggccatca acatcaggct
     5281 gatagcgata accacggcgg ccatcatgac cagtaaatgg tcgaccatcc cgacggcggt
     5341 aatcactgag tcgagtgaga aaatggcgtc cagtaccaca atttgcgtca ccacgcccca
     5401 gaacttcgca cctttgcgtt gtgtgggatt attgctgtct ttcccttcca gccgttcgtt
     5461 cagctccatc gtggctttga acaacaggaa gaaaccacca aacagcatga ttaagtcgcg
     5521 ggcgctaaag gtaaacgagc ggaagctgaa cagcggttga gtcagggtga ccagccagga
     5581 gattgacgcc agcagtaaca ggcgcattaa catcgccagc agcagcccgg taacccgtgc
     5641 gcggtcgcgc tgcttcggcg gtagtttttc ggcgaggatg gcaataaaga ccaggttatc
     5701 aatgccgagg accagttcga tcacaatcag cgtgattaac ccggcccaga tagacggatc
     5761 ggcaatccat tccatagtaa gtacaaaacc tattcgttgt atgacaaatg tcacaattcg
     5821 atcatggata aatcggggca aaaatgcaa
//
LOCUS       DAZFAH010000244         5838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFAH010000244 DAZFAH010000000
VERSION     DAZFAH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.246"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..704
                     /locus_tag="ACHJWB_004357"
     CDS             33..704
                     /locus_tag="ACHJWB_004357"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO4315873.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            714..1910
                     /gene="yaiP"
                     /locus_tag="ACHJWB_004358"
     CDS             714..1910
                     /gene="yaiP"
                     /locus_tag="ACHJWB_004358"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO4315874.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1888..2469
                     /locus_tag="ACHJWB_004359"
     CDS             1888..2469
                     /locus_tag="ACHJWB_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4315875.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2471..3244
                     /gene="yaiO"
                     /locus_tag="ACHJWB_004360"
     CDS             2471..3244
                     /gene="yaiO"
                     /locus_tag="ACHJWB_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO4315876.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3429..3704
                     /gene="frmR"
                     /locus_tag="ACHJWB_004361"
     CDS             3429..3704
                     /gene="frmR"
                     /locus_tag="ACHJWB_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4315877.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3739..4848
                     /gene="frmA"
                     /locus_tag="ACHJWB_004362"
     CDS             3739..4848
                     /gene="frmA"
                     /locus_tag="ACHJWB_004362"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4315878.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHGGKSIRTVIRY"
     gene            4942..5775
                     /gene="fghA"
                     /locus_tag="ACHJWB_004363"
     CDS             4942..5775
                     /gene="fghA"
                     /locus_tag="ACHJWB_004363"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4315879.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
BASE COUNT         1434 a         1390 c         1494 g         1520 t
ORIGIN      
        1 gtagtataat aaataaaatg gagtgtcatt aagtggataa ggttttagat tcagccctcc
       61 tttcttcggc aaataaaaga aagggtatcc ttgctattgg cgcacatcct gacgatatag
      121 aattaggctg tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat atcgcagccg
      181 tggtgatgac taccggcaac tctggtacag atggaataat cgatcgccat gaagaatcgc
      241 gcaacgccct aaagatatta gggtgccacc aaactattca tcttaatttt gctgacaccc
      301 gcgctcattt acagctcaat gatatgattt ccgctctgga agacatcatt aaaaatcaaa
      361 ttccttctga tgttgaaatc atgcgggtat ataccatgca tgatgccgac cgccatcagg
      421 atcatctcgc tgtttatcaa gcttcaatgg ttgcctgccg cactattcca caaattctcg
      481 gctacgaaac cccaagtacc tggctttcat ttatgcctca ggtttttgaa tccgttaaag
      541 aagaatattt cacagtcaaa cttgctgcat taaaaaaaca taaaagccag gaacgacgtg
      601 attatatgcg tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa caggtcaata
      661 gcgatctggg tgaaggcttt gtaattcata aaatgattct ttgagcaaat tgcatgaaaa
      721 cctggatatt tatctgtatg tccatagcaa tgttgctatg gtttttaagt acgctaagac
      781 gtaaacccag tcaaaagaaa ggctgtattg acgccattat acctgcgtat aacgaaggcc
      841 cgtgtctggc gcagtcactg gataatctac tgcgtaaccc ttatttttgc cgggtaattt
      901 gcgttaacga cggctccacg gacaataccg aagcggtcat ggcggaagtc aaacgcaaat
      961 ggggcgaccg ctttgttgcc gtcacgcaaa aaaataccgg taaaggtggt gcgctgatga
     1021 atggcctcaa ttatgccacc tgcgaccagg tttttttaag tgatgccgac acctatgttc
     1081 cgcccgatca agacggaatg ggctatatgc tggcagaaat tgagcgcggt gctgatgccg
     1141 taggcggcat tccctctact gcgttgaaag gcgcggggct gttaccgcac atccgcgcga
     1201 ccgtaaagtt gccgatgatt gttatgaagc gcacgctaca gcagctcctg ggcggcgcac
     1261 cgtttattat cagcggtgcc tgcgggatgt tccgtactga tgtattgcgt aagttcggtt
     1321 tctcggatcg tactaaagtc gaagaccttg atctcacctg gacattagtg gcaaacggct
     1381 accgtattcg gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac agcccgcgtg
     1441 aggagtggcg tcgctggcgg cgttggattg tgggatacgc ggtctgtatg cgcctgcata
     1501 aaagactttt atttagccgc ttcggtatct tcagtatatt tcctatgctg ttggttgtga
     1561 tttatggcgt tgggatttat ctcactacct ggtttaatga attcatcacc accgggccgc
     1621 atggtgtggt gttggcaatg tttccgctta tctggatcgg cgtagtttgt gttattggtg
     1681 cttttagcgc ctggtttcat cgttgctggt tgttggtgcc tttagcgccg ctttccgttg
     1741 tgtatgtatt attagcttat gccatctgga ttatttatgg acttattgcc ttttttactg
     1801 gacgcgaacc tcagcgcgac aaacccaccc gctattccgc actggtggaa gcgtcaaccg
     1861 cttattccca accttctgtc acaggaactg aaaaactatc tgaagcttaa tgttaaagag
     1921 aaaaacattc agattgcaga ccaggtaatt attgatgaaa ctgcaggtga agttgttatc
     1981 ggcgcgaata cccgtatttg tcatggtgcc gttattcagg gtccggtagt gattggcgca
     2041 aactgcctga taggtaatta tgcgtttatt cgtcctggca caataatcag caatggtgta
     2101 aaaattggtt ttgccaccga aattaaaaat gcggttattg aagcggaagc aacgattggt
     2161 ccgcaatgtt ttattgccga ctcggtagtt gcaaaccagg catatttggg cgcacaagta
     2221 cgtaccagta atcatcgtct ggatgaacag cctgtgtctg ttcgaactcc agagggaatt
     2281 atcgctaccg gatgcgataa attaggttgt tatatcgggc agcgttcacg ccttggcgta
     2341 caagttataa ttttgcctgg gcgaattatt tccccgaaca cgcaacttgg cccgcgcgtg
     2401 attgtagaac gtaatttacc tgccggaact tattcactcc gacaagaact tatccgtaca
     2461 ggagattaaa atgataaaac ggacgttatt agcggcggcc atttttagcg cattgcccgc
     2521 ttatgccggg ttaacttcca ttaccgcggg ctacgatttt accgattatt ctggcgatca
     2581 tggcaaccgt aatttagcgt atgctgaact ggtggcgaaa gttgaaaacg caacgctgct
     2641 ttttaatctt tcccaggggc gtcgtgatta tgaaactgaa catttcaatg ccactcgcgg
     2701 tcagggtgcg gtctggtata aatggaataa ctggctgaca acccgaacgg gtattgcctt
     2761 tgcggataat acgccggtct ttgcccgcca ggattttcgt caggatatta acctggccct
     2821 gttgccaaaa acgcttttca cgaccggtta tcgctacact aaatattacg atgatgtcga
     2881 agtcgatgcc tggcaaggcg gcgtatcact ctatactggc ccggtgatca ccagctaccg
     2941 ctatacccat tatgactcca gcgatgcagg tggtagttat agcaatatga tttccgtgcg
     3001 tctgaatgac ccgcgcggca ctggttatac gcaactatgg ctaagccgcg gaacaggtgc
     3061 ttacacctat gactggacgc cagaaacacg ctacggcagc atgaagagcg tcagtctgca
     3121 acgtattcaa ccgctgactg agcaacttaa tctcgggctg acggcaggta aagtgtggta
     3181 cgacacccca accgatgatt ataacggtct gcaacttgca gcccatctga cctggaaatt
     3241 ctgattcctt ctgccgcccg ctatccgggg cggccttccc tgccgattag cccccccttt
     3301 cctctttgtt ttccgaccac attcaccgga taaattttat tctccagtgt tatatactat
     3361 aggggggtat gcattgacat atagcatacc cccctatagt atattgcgtg cagatgatga
     3421 ggtgcgaaat gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta
     3481 ttcgagggca gattgatgct ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca
     3541 tactccaaca gatcgctgcc gttcggggcg cggctaacgg gctgatggca gaagtgcttg
     3601 aaagccatat ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat
     3661 ccgttgacga cactattgaa ctggttcgag cctatcttaa atagctgaat ctattaccat
     3721 attgaggaag agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg
     3781 ctggaaatcg ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc
     3841 acccataccg gcgtttgcca taccgacgca tttaccctct ccggggatga cccggaaggt
     3901 gtattcccgg tggttctcgg tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc
     3961 gtaaccagcg tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag
     4021 tgtgagttct gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt
     4081 aaaggcttga tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac
     4141 tacatgggat gctcaacatt cagtgaatac accgtggtcg cggaagtgtc tctggcaaaa
     4201 attaatccag aagcaaacca tgaacacgtc tgcctgctgg gctgtggcgt gaccaccggt
     4261 attggcgcgg tacacaacac agctaaagtc cagccaggtg attctgttgc cgtgtttggt
     4321 cttggcgcga ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggtcggatt
     4381 atcgctattg atacgaaccc gaagaaattc gatctggcgc gtcgcttcgg tgccaccgac
     4441 tgcattaacc cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa
     4501 tggggtatcg accatacctt tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg
     4561 gaaagtgcgc accgcggctg gggtcagtcg gtgatcatcg gggtcgccgg ttctggtcag
     4621 gaaatctcca cccgaccatt ccagttggtc accggtcgcg tatggaaagg ttccgcgttt
     4681 ggcggcgtga aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat
     4741 atcgatctgg aaccgtttgt cacgcatacc atgagcctgg atgaaattaa tgacgccttc
     4801 gacctgatgc atggaggcaa atccattcga accgtaattc gttactgatt tcccgcaggt
     4861 ttaccccgtc cagttcaggc gggggttctt aatactctcc ccgggcagcc gtccggggga
     4921 ttaaccgtga gataatgact gatggaactc attgaaaaac atgccagctt tggcggctgg
     4981 caaaatgtgt atcggcatta ttcccaatca ctgaaatgtg aaatgaatgt cggcgtctat
     5041 ctcccaccaa aagccgcgaa tgaaaaattg ccggtgctgt actggctttc aggcctgacc
     5101 tgcaacgagc agaatttcat tactaaatcg gggatgcagc gttacgcggc tgaacacaac
     5161 attattgttg ttgcgccgga caccagtccg cgaggcagtc atgtcgcaga tgccgaccgt
     5221 tacgatctcg ggcaaggtgc cgggttttac ctgaacgcaa cgcaagcgcc gtggaatgaa
     5281 cattacaaaa tgtatgacta tatccgcaac gagctgccgg atttagtgat gcaacatttt
     5341 ccggcaacga ccagaaagtc tatctctggt cattctatgg gcgggctggg cgcgctggtg
     5401 ctggcgttac gtaacccaga tgaatatgtc agcgtctcgg cgttttcgcc cattgtctcc
     5461 ccatcgcaag taccgtgggg acagcaagcc tttgccgcat atcttggtga aaataaagat
     5521 gcctggttgg attacgatcc ggtgagtctt atttcacaag gtcaacgcgt tgcggaaatc
     5581 atggttgatc aggggttgag tgatgatttt tacgcggaac agctgcggac tccaaatctt
     5641 gaaaagatct gccaggagat gaatatcaag acgttaatcc gttatcacga gggttacgac
     5701 cacagctatt attttgtctc cagttttatt ggcgagcata ttgcctacca cgccaataaa
     5761 ctgaatatgc gttgataata gtgcacgact gccggatgcg gcgtgaacgc cttatccggc
     5821 ctacacttcg cccgtaaa
//
LOCUS       DAZFAH010000245         5818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFAH010000245 DAZFAH010000000
VERSION     DAZFAH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.247"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..1041
                     /gene="ldhA"
                     /locus_tag="ACHJWB_004364"
     CDS             52..1041
                     /gene="ldhA"
                     /locus_tag="ACHJWB_004364"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4315880.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKSETCPNELV"
     gene            1152..1574
                     /gene="hslJ"
                     /locus_tag="ACHJWB_004365"
     CDS             1152..1574
                     /gene="hslJ"
                     /locus_tag="ACHJWB_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4315881.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1571..1837)
                     /locus_tag="ACHJWB_004366"
     CDS             complement(1571..1837)
                     /locus_tag="ACHJWB_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4315882.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2111..5635
                     /gene="nifJ"
                     /locus_tag="ACHJWB_004367"
     CDS             2111..5635
                     /gene="nifJ"
                     /locus_tag="ACHJWB_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4315883.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1474 a         1493 c         1490 g         1361 t
ORIGIN      
        1 aaaatatttt tagtagctta aatgtgattc aacatcactg gagaaagtct tatgaaactc
       61 gccgtttata gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa cgagtccttt
      121 ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa aacggccaat
      181 ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccgcccggt gctggaagag
      241 ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa taacgtcgac
      301 cttgacgcgg caaaagaact ggggctgaaa gtagtccgtg ttccagccta tgatccagag
      361 gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat tcaccgcgcg
      421 tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggctt tactatgtat
      481 ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct gcgcattctg
      541 aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcagc ggcgctggaa
      601 ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt tatctctctg
      661 cactgcccgc tgacaccgga aaactaccat ctgttgaacg aagccgcctt cgatcagatg
      721 aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc tcaggcagca
      781 attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtgta tgagaacgaa
      841 cgcgatctgt tctttgaaga taaatccaac gacgtgatcc aggatgacgt attccgtcgc
      901 ctgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac agcagaagct
      961 ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa aagcgaaacc
     1021 tgcccgaacg aactggttta atcttgccgc tcccctgcat tccaggggag ctgattcaga
     1081 taatccccaa tgacctttca tcctctattc ttaaaatagt cctgagtcag aaactgtaat
     1141 tgagaaccac aatgaagaaa gtagccgcgt ttgttgcgct aagcctgctg atggcgggat
     1201 gtgtaagtaa tgacaaaatt gctgtaacgc cagaacagct acagcatcat cgctttgtgc
     1261 tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa tccgccagaa atcagctttg
     1321 gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa ggcaaactgt
     1381 ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc gctaacccgc
     1441 agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca caagtggatc
     1501 tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat aagctggcgg
     1561 atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttca ctacagcgtt
     1621 tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg gcaacagaaa
     1681 gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac gcttttaaac
     1741 ctggcgctac gtgcgccgca gtcgcctgct gaacaggctc actactacac gccgacaaca
     1801 ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct tatcttcaag
     1861 ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga gctgtcctgc
     1921 gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt tgatcaaaac
     1981 attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc catttgctaa
     2041 aattgaaaga ttactactgg gcgcgcagca atttcgtgcg cccctcattt gcgcaatgta
     2101 agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg cgtttcgcac
     2161 cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag aacaggctga
     2221 tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg tggttgaaat
     2281 gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg gtgccctttc
     2341 aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt acaaactggc
     2401 aggcgaactc acaccgtttg tcctgcatgt agcggcacgt accgttgcca cacatgcact
     2461 ctctattttt ggcgatcatt ccgacgtcat ggcggtgcgc cagacgggtt gcgcgatgtt
     2521 gtgtgcggca aacgtccagg aagcgcaaga ctttgctctc atttcgcaca tcgcgacgct
     2581 gaaaagccgc gtgccattta ttcatttctt tgatggtttc cgcacgtccc acgaaatcaa
     2641 taaaattgtc ccgctggccg atgacacgat tcttgatctc atgccgcagg ccgaaattga
     2701 tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgtggta cgtccgccaa
     2761 tcctgacact tatttccagt ctcgcgaagc caccaacccc tggtacaacg cagtctatga
     2821 ccatgttgaa caggcgatga atgatttctc tgccgcgaca ggtcgtcagt atcagccgtt
     2881 tgaatattac gggcatccgc aagctgaacg ggtgattatc ctgatgggct cagccattgg
     2941 cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagtcg gcgtgctgaa
     3001 agttcgcctg taccgcccct tctcagccaa acacttgcta caaactctac cgggatccgt
     3061 acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag aaccgctcta
     3121 tctggatgtg atgaccgcac tggcagaagc ctttaataat ggcgagcgtg aaaccctgcc
     3181 ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg actgtgtact
     3241 ggcggtattt gccgagctca acgcggctaa accgaaagcg cgctttacgg ttggtattta
     3301 cgatgatgtg actaatctgt cactgccgtt gccggaaaac accctgccaa actcggcgaa
     3361 actggaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcga ccaaaaacaa
     3421 tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg tttacgactc
     3481 caaaaaggcg ggcggcctga cggtttctca ccttcgagtg agcgaacagc cgattcgttc
     3541 cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt ttatcgataa
     3601 atatcagatg gctgagcgtt taaaacctgg cggcattttc ctgctcaaca caccgtacag
     3661 cgcagatgaa gtgtggtcgc gcttgccgca agaagttcag gccgtgttaa accagaaaaa
     3721 agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc tggcggcccg
     3781 tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc ctggcgatag
     3841 cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag gccaggatct
     3901 ggtggaacgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag aagttccgtt
     3961 gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg ccgcccctga
     4021 tttcgtgaaa accgtaaccg ctgcgatgct cgccgggctt ggtgatgccc tccccgtttc
     4081 ggcgctgccg ccagacggca cctggccgat gggcactacg cgctgggaaa aacgcaatat
     4141 cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc actgcgtcgc
     4201 cgcttgccca cactcagcta ttcgcgcaaa agtggtacca cccgaagcga tggaaaacgc
     4261 ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat atgcgcgggc agaaatatgt
     4321 cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag tttgcccggc
     4381 gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc tggaacatgt
     4441 cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg accgtagcaa
     4501 actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg aatattcagg
     4561 tgcttgctcc ggttgtggcg agacgccgta tattaaattg ctgactcagc tctatggcga
     4621 ccggatgttg atcgctaacg ccactggctg ttcttcaatt tatggcggta acctgccctc
     4681 tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact ctctgtttga
     4741 agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc gtgtccgcgt
     4801 gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga cagcgttgaa
     4861 atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc agcaactcaa
     4921 cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg aaaaatcaat
     4981 ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc tggatcatgt
     5041 attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct attccaacac
     5101 cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaaatttg gcgagcacgg
     5161 caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc atgtttatgt
     5221 ggcgcagatt tctctcggcg cgcagctcaa ccagacggtg aaagcgattc aggaagcgga
     5281 agcgtatccg gggccatcgc tgatcatcgc ttatagccca tgtgaagagc atggttacga
     5341 tctggcactc agccacgacc agatgcgcca actcacagct accggcttct ggccgctata
     5401 tcgcttcgat ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg attcacgccc
     5461 gccgtcagaa gcactggaag aaacgttact tcacgagcaa cgtttccgtc ggctgaattc
     5521 acagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt tgcaaaaacg
     5581 ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa agcaacaccg attaaatgct
     5641 ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg gcttgtcttt
     5701 ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa
     5761 taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatt
//
LOCUS       DAZFAH010000246         5810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFAH010000246 DAZFAH010000000
VERSION     DAZFAH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.248"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..469)
                     /locus_tag="ACHJWB_004368"
     CDS             complement(<1..469)
                     /locus_tag="ACHJWB_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315884.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYD"
     gene            complement(722..1345)
                     /locus_tag="ACHJWB_004369"
     CDS             complement(722..1345)
                     /locus_tag="ACHJWB_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4315885.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(1342..2007)
                     /locus_tag="ACHJWB_004370"
     CDS             complement(1342..2007)
                     /locus_tag="ACHJWB_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO4315886.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(2004..2624)
                     /locus_tag="ACHJWB_004371"
     CDS             complement(2004..2624)
                     /locus_tag="ACHJWB_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4315887.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVNAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            complement(2617..2787)
                     /locus_tag="ACHJWB_004372"
     CDS             complement(2617..2787)
                     /locus_tag="ACHJWB_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4315888.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSPMHEEEDDG"
     gene            complement(2784..2966)
                     /locus_tag="ACHJWB_004373"
     CDS             complement(2784..2966)
                     /locus_tag="ACHJWB_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4315889.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            complement(2963..3403)
                     /locus_tag="ACHJWB_004374"
     CDS             complement(2963..3403)
                     /locus_tag="ACHJWB_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4315890.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(3477..3767)
                     /locus_tag="ACHJWB_004375"
     CDS             complement(3477..3767)
                     /locus_tag="ACHJWB_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4315891.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(3764..4465)
                     /locus_tag="ACHJWB_004376"
     CDS             complement(3764..4465)
                     /locus_tag="ACHJWB_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4315892.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(4462..5361)
                     /locus_tag="ACHJWB_004377"
     CDS             complement(4462..5361)
                     /locus_tag="ACHJWB_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4315893.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYVRLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(5394..5690)
                     /locus_tag="ACHJWB_004378"
     CDS             complement(5394..5690)
                     /locus_tag="ACHJWB_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4315894.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
BASE COUNT         1326 a         1494 c         1279 g         1711 t
ORIGIN      
        1 tatcatagaa gcatgatacc gataaaaatc gcccataaga tcttatcgaa attgtaaccg
       61 ccagcgcaat cgctgctgac agtagcaaaa gaggtacctg aatcttctgt ctcaatatag
      121 aaaactcaat aattgccggc acaaacaata attccacagc aaaataaagg cgaaatacat
      181 ttagctcttg catagaatgt tttcttttca ctgcgaaaaa gaatacaaca ccaatacccc
      241 aaccgataag aaatatagca atgacgataa ctgcaaaaaa taaacttctg gcaacatcat
      301 caacacctgc acctacaatc caccatggga agccgtagta aaaagaagta ccccatccat
      361 agaaataagc actcccccat ccaaggcatc ccatgtaggc aataaaaagt gaagaactcc
      421 tgagcagcgc accatccttc ataaccaccc caatacaaga tgataacatt ggcttacaac
      481 tcataacaaa agcaattcaa tgccgtcaag aggttacagg ctaaaaaaac tctattacat
      541 tgcagtcagc atgtttacta cacaaataca attcagagca taaaaactac tcggcggcag
      601 gttattgaga ctcatcaatg acatgtaaaa aacgcccatt attggtgtca agtttcccca
      661 aagttattca aaaagtcaat attatgccgt taatatgttg ccatccgtgg caatcatggc
      721 gctaacgtgt gatcgcattc aaaatgttgt ctgcgattga ctcttccttg tggcattgca
      781 caaccagagc gtcatacagc ggcttaacag tgcgtgacca ggtgggttgg gtaaggtttg
      841 ggattagcat cgtcacagcg cgatatgcgg cgcttgctgg cattcttgaa taaccgacgc
      901 ctttacatct tccgcactct ttctcagcaa ctatccccca ctgctctgtt ttggctatat
      961 caaccgcacg gcctgtaccg tggcaatctc tgcatcttgc gcccggcgta gcggcactac
     1021 ggcaataatc cgcataagcg aatgttgcga gcacttgcag tacctttgcc ttagtatttc
     1081 cttcaagctt tgccacgcca cggtatttcc ccgatacctt gtgtgcaaat tgcatcagat
     1141 agttgatagc cttttgtttg tcgttctggc tgagttcgtg cttaccacag aatacagcca
     1201 ttccgaatcc ggcttgtgat tgcgccatcc ccatagcagc catcacatca gtaccggaaa
     1261 gagagtcaga agccgtggcc cgtggtgagt cgctcatcat cgggcttttt ggcgaatgaa
     1321 atttagctac gctttcgagt ctcatgcgcc ttctccctgt acctgaatca atgtgaggtt
     1381 tccgcagaac actgcgccag tatcgatata catctggttg gcaaatttga gtggtttcac
     1441 tgctggcgta tgaccaaaga tgaacgtgtc cgcgcctttg atttctttca cgatcccgtc
     1501 ttgtgagttg ctgattcgtt cgcggttcca gattacctgc tgatgatcaa ctggctttcc
     1561 aaactcgtat ttatcacaag gataatcggc gtggcagatg acatattttt ttcctttact
     1621 caccagttcg atgattaacg gaagttcatc tgctttatgg gcaagagctt tagccagaat
     1681 ttctttgtcg taatcgagat taaagaacca gccaccgcca ttaagcagcc agtgattgac
     1741 gtttccacgc tctgataagc catcaatcat catttgctca tggtttccac gtacagctct
     1801 gaaccagggg aatgtgatta attccaggca ttctacgttc tctgtaccgc gatcaaccaa
     1861 atcgcccacc gagataagca ggtctttttt ggtgtcgaat cctatcgtct ccagtttttt
     1921 catcaggttc gtgtagcatc cgtgcagatc gccaactacc caaatatttc ggtatttgct
     1981 gccatcaatt ctttcgtaga tattcatgca acctcacttc tgctgtttcg cagtttttta
     2041 agtttctgtt gatactccgc cttgatggcc ctgcactctt cgacagtcca gcgatagcgg
     2101 ttatggtttg attcgatttc ctctactgct tcctgcccga tgcggctaat cagttcgacg
     2161 cgatacggaa cgagatttcc gcttttgtgc tggttgcaca ccacgcattg cttgtgaata
     2221 ttgcgttcat caaatcggag ttgaggtgcc gcagcagttg tccggtaatg tccggcatcc
     2281 cactgagcag acgtgagcgt tccgcacgag atacatggta agtcgcggtc tctttctctg
     2341 atgaaggcgt ttacggcttg ttgggcttgt ttaatccagt aactgcgggg ctttaaggcg
     2401 agtttttgaa tcttaagttt atctttctgt ttctgctcct ctcgtcgtcg tttcttctct
     2461 gctgcctttt ctgccttttc cgctttttcg cgttctttgc ttcgtcgttc gagtgctatc
     2521 ttggttccac actctggaga gcaccaccac tgattagcga atgcagggtg aaaccattcc
     2581 cgacattcat cgtttttaca tcgtcttcgc gctggtttag ccatcatctt cttcctcgtg
     2641 catcgggcta ttcgaatcgc tcatcagctc tgcgcagcag tgctcacaca cgtgaacttc
     2701 cagcacatgc agcttctgac cgcagttagc gcacgttaaa gctcgctcga cgctttcttg
     2761 ttcgtaactt cgattttggt caatcacctt gttttcctcg cacaatgtct tagccaccgg
     2821 atatcccaca ggtgagccgt gtagttgaag gtttttacgt cagattcttt tgggattggc
     2881 ttgcgtttat ttctggagcg tttcgttgga aggtatttgc agttttcgca gatgatgtcg
     2941 gtgaaacttc gtcgctgtcg tctcatgccg ccctgtctcc ccatcgcgct ttccattcga
     3001 gagccagtcg cgcttcgtct gaccacttaa cgccacgctc tgtaccgaat gcctgtataa
     3061 gctctaatag ctccgcaaat tcgcttacac gcatcctgct ggttgactgg cctattacca
     3121 caaagccatt cccggcaagg ttaggaacaa catcctgctg ctttaatgct gcggtaaaca
     3181 cacacttcca gctttctgca tccagccagc gaccatgcca ttcaacctga cgagagacgt
     3241 cacctaagca ggcccatagc ttcctgtttt ggtctaagct gcggttgcgt tcctgaatgg
     3301 ttactacgat tggtttggtt gggtctggaa ggatttgctg tactgcgtga atagcgtttt
     3361 gctgatgtgc tggagatcga atttcaaagg ttagtttttt catgacttcc ctctccccca
     3421 aataaaaagg cctgcgatta ccagcaggcc tgttattagc tcagtgatgt agatggtcat
     3481 cttttaactc catataccgc caatacccgt ttcatcgcgg cactctggcg acactcctta
     3541 aaaatcaggt tcgtgctcat ctttccttcc cgttcttccc tggtagcaaa ccggtaatac
     3601 accgttcgcc agaccttacc ttcgataacc agaagacctg cccgtgccat tttagccgcg
     3661 gcctgattta tgctggttac tgttgcgcct gttagcgcgg caacgtccgg cgcacagaag
     3721 ctattatgcg tccccaggta atgaataatt gcctctttgc ccgtcataca cttgctcctt
     3781 tcagtccgaa cttagctttg atttctgcga tcttcgccag agcctgtgca cgatttagag
     3841 gtctaccgcc catgacagga agttgtttta ctggttcagg gatcgcctca ccacggttaa
     3901 ttctcgcagt catatggaca agctcatctg cggccttacg gcgtaattcc gcatcagtaa
     3961 gcgcattggc ccgcatgttc tgatacaggt tggtaaccag ccagtagtgc gcgtttgatt
     4021 tccacggata agactccgca tccggataca ggcctcgctt ccggcaatac tcgtaaacca
     4081 tatcaaccag ctcgctgacg tttggcagtc cggcgataac ggatgcttct tcccggcacc
     4141 atgcaacaaa ctgcccgggt gatggcagaa atggtcgatt ctgccgacgg gctacgcgca
     4201 ttcctgcgtt aacctgttcc attgtggtga tcccgttttc ccggaaagcc agaacccact
     4261 ggcggcggat ttcgttcagt tcgttctggt cacggttagc caggctcgcc gggaaagttg
     4321 ccagtaactg gctgaacaca ccgttgatga tctgcgctac ctgctgtacc tgcggctttt
     4381 cgtcgtactg ttccggcatg ttgttggcga ttcgacgcat ctgctcacgg tcaaagttaa
     4441 ccatctgtgc ggcgatgttt ttcataaatc caccccgtaa atccagtcag tgtttgtcag
     4501 gtcgagtttt ggtttgctgg ctgtcacgcc tgcctgttgc ttgttacggt tgatttcgag
     4561 ctgggtccac ttgtcgcgga gtttggccgg actcagcacg ttaccggacc agaagttgtc
     4621 ctggcaggcc cagcggaaaa gcacacacat atcgcggtgg ttacgtccgt cacgttcacg
     4681 catcaggcgg atatcgttag cccacccagc aaaattcggt tttctggctg atggcgcgat
     4741 agtcttcacc atgtcaaaca tccactctgc ggcggtcagg tcttctgctg tcccccactt
     4801 gctgccgctc tgaattgcag catccggttt aaccacagaa aggtcgtttt ctggctggtc
     4861 agaggattcg ccagaattct ctgacgaata atcttttctt ttttcttttg taatagtgtc
     4921 ttttgtgtcc ccctgttttg agggatagca atcccccaat ttgagggatg ttttatccct
     4981 cgttttaggg gattttccct cgttttgagg gatgcaccat tctgagatgt ttttgtttgg
     5041 tccaaacatg ccgccttgct gcttgataat attcattctg acgagttcta acttggcttc
     5101 attgcaccgt ttgacaggta actttgtaat ctcgctaagt tgagaatcgg tgattctgtc
     5161 cattggttta ttccacccat aggttttacg cagaatggca agcagcactt taaactgtcg
     5221 cttggtcaga tctgcgcctg aataagcctc aagcagcata tttgatagtc tgacgtaacc
     5281 atcatcgaga tctgccacat tacgctcctg ttcggcaaag ttacctctgc cgaagttgag
     5341 tatttttgct gtatttgtca taatgagtcc tgtggattga tccagtaatt ccctcagaat
     5401 tgcatatcaa tttgcttaaa atcctcggtg gcagccgggg attttttctt tgtgatttca
     5461 tcaagcgcat acttaaaagc cctgctaatc ggactgatgt ctgatgccat tccgaaagca
     5521 cacaagaccg aagcaataaa tctccagtcc gttctgctta tcttcgattc atgacagcca
     5581 atcatctttg ccaaaccgcg ctgtgtaagc gttgataggt tgatgagtaa atctgtttct
     5641 gcgcgatcaa cgtcacgctg tgatagtttg ctgtaacttg ttctttccat ttcttaagat
     5701 ttccaatagt gaatagttag ttgaaaggta tgcgtggaaa cgcatatggc cttagttggt
     5761 cagatatctt gggactcgct tttcagcgac gtaggacgaa tgtccgttgt
//
LOCUS       DAZFAH010000247         5792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFAH010000247 DAZFAH010000000
VERSION     DAZFAH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.249"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..807
                     /gene="fucA"
                     /locus_tag="ACHJWB_004379"
     CDS             160..807
                     /gene="fucA"
                     /locus_tag="ACHJWB_004379"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4315895.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            835..1983
                     /gene="fucO"
                     /locus_tag="ACHJWB_004380"
     CDS             835..1983
                     /gene="fucO"
                     /locus_tag="ACHJWB_004380"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4315896.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALNDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2038..2793)
                     /gene="xni"
                     /locus_tag="ACHJWB_004381"
     CDS             complement(2038..2793)
                     /gene="xni"
                     /locus_tag="ACHJWB_004381"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4315897.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2905..4272)
                     /gene="sdaB"
                     /locus_tag="ACHJWB_004382"
     CDS             complement(2905..4272)
                     /gene="sdaB"
                     /locus_tag="ACHJWB_004382"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4315898.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4330..5619)
                     /gene="sdaC"
                     /locus_tag="ACHJWB_004383"
     CDS             complement(4330..5619)
                     /gene="sdaC"
                     /locus_tag="ACHJWB_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4315899.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1432 a         1452 c         1566 g         1342 t
ORIGIN      
        1 agttatttac tgtgattagt tgaaccaggt cacaaaatat aagcttgtga atttctttct
       61 ggttaatcag gaagaagatg gtgggtaatt aaacggctaa ttcaatagtg tgaaaggaac
      121 aacattattg ccctgttttg aatcagagag aggtagctaa tggaacgaaa taaacttgct
      181 cgtcagatta ttgacacttg cctggaaatg acccgcctgg gactgaacca ggggacagcg
      241 gggaacgtca gtgtacgtta tcaggatggg atgctgatta cgcctacagg cattccatat
      301 gaaaaactga cggagtcgca tattgtcttt attgatggca acggtaaaca tgaggaagga
      361 aagctcccct caagcgaatg gcgtttccat atggcggcct atcaaagcag accggatgcc
      421 aacgcggttg ttcacaatca tgccgttcat tgcacggcag tttccattct taaccgatcg
      481 atccccgcta ttcactacat gattgcggcg gctggcggta attctattcc ttgcgcgcct
      541 tatgcgacct ttggaacacg tgaactttct gaacatgttg cgcaggctct caaaaatcgt
      601 aaggcaactt tgttacaaca tcatgggctt atcgcttgtg aggtgaatct ggaaaaagcg
      661 ttatggctgg cgcatgaagt tgaagtgctg gcgcaacttt acctgacgac cctggcgatt
      721 acggacccgg tgccagtgct gagcgatgaa gagattgccg tagtgctgga gaaattcaaa
      781 acctatgggt tacgaattga agagtaattt cgtaaagcaa caaggagaag gatgatggct
      841 aacagaatga ttctgaacga aacggcatgg tttggtcggg gtgctgttgg ggctttaacc
      901 gatgaggtga aacgccgtgg ttatcagaag gcgctgatcg tcaccgataa aacgctggtg
      961 caatgcggcg tggtggcgaa agtgaccgat aagatggatg ctgcagggct ggcatgggcg
     1021 atttacgacg gcgtagtgcc caacccaaca attactgtcg tcaaagaagg gctcggtgta
     1081 ttccagaata gcggcgcgga ttacctgatc gctattggtg gtggttctcc acaggatact
     1141 tgtaaagcga ttggcattat cagcaacaac ccggagtttg ccgatgtgcg tagcctggaa
     1201 ggactttccc cgaccaataa acccagtgta ccgattctgg caatccccac cacagcaggc
     1261 actgcggcag aagtgaccat taactacgtg atcactgacg aagaaaaacg gcgcaagttt
     1321 gtttgcgttg atccgcatga tatcccgcag gtggcgttta ttgacgctga catgatggat
     1381 ggtatgcctc cagcgctgaa agctgcgacg ggtgtcgatg cgctcactca tgctattgag
     1441 gggtatatta cccgtggcgc gtgggcgcta accgatgcac tgcacattaa agcgattgaa
     1501 atcattgctg gggcgctgcg aggatcggtt gctggtgata aggatgccgg agaagaaatg
     1561 gcgctcgggc agtatgttgc gggtatgggc ttctcgaatg ttgggttagg gttggtgcat
     1621 ggtatggcgc atccactggg cgcgttttat aacactccac acggtgttgc aaacgccatc
     1681 ctgctaccgc atgtcatgcg ctataacgct gactttaccg gtgagaagta ccgcgatatc
     1741 gcgcgcgtta tgggcgtgaa agtggaaggt atgagcctgg aagaggcgcg taatgccgct
     1801 gttgaagcgg tgtttgctct caaccgtgat gtcggtattc cgccacattt gcgtgatgtt
     1861 ggggtacgca aggaagacat tccggcactg gcgcaggcgg cactgaatga tgtttgtacc
     1921 ggtggcaacc cgcgtgaagc aacgcttgag gatattgtag agctttacca taccgcctgg
     1981 taaatgcgct gatgtgataa tgccggatac gacgtttgcg gcgtatccgg ctcgccgtta
     2041 ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc aaatcggttt gtaagcgggc
     2101 aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc gccacttttc
     2161 ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga ccagcagctg
     2221 cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc tgatccccgc
     2281 aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat cgataaatgg
     2341 cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg aaagtaactg
     2401 acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggcctgtg tcactttgac
     2461 cgccagcgtg gcggctaagt catcggcttc gttgccgctg gttgaccagc acgggacgcc
     2521 gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt ccggcattgg
     2581 cggtcgaccc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac tgcggttttc
     2641 atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc ataatgagct gatcgagcgc
     2701 atgctggcag gtttcgacac agggcgatcc ctgaacggca tgaatgcgac gaataagatt
     2761 cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga tcaggcgata
     2821 agggtaacat tcgatgccgg aacaggctac ggctggatga gaaatcggga agaggcctcg
     2881 caaaacgagg cctctggaga gcgattaatc gcaggcaacg atcttcattg ccaggccgcc
     2941 gcgagaggtt tcgcggtact tggcgttcat atctttacct gtttcgtaca tggtttcgat
     3001 aactttatcg aggcagacgc gcggctcgct ggtacggcgt agcgccatac gtgcggcgtt
     3061 caccgctttt actgccgcaa tggcgttacg ctcgatgcat ggcacctgga cctgtccggc
     3121 gaccgggtca cacgtcagac cgaggttgtg ctccatggcg atttccgccg cgatgcacac
     3181 ctgcgccggg cttgcaccta acagttctgc cagaccagcc gccgccattg agcacgccac
     3241 accaacttca ccctggcagc cgacttcagc accagaaatt gacgcgttca tcttataaag
     3301 agaaccaatg gcactggcta ccagcaggta acgagccagt gagttagcgt tcacttcgcg
     3361 gataaacttg tcgtagtacg ccagcactgc cgggataatc ccgcacgcac cgttagtcgg
     3421 cgcagtcacc acgcgtccgc cagcagcgtt ctcttcgttc actgccagtg caaacatgtt
     3481 gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga ccagcatccg
     3541 gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggcaacacgc cttcggtgga
     3601 aataccgcgc tcaataccgc cacgcatgac ttcccatacg ttcgccaggt gctgttccag
     3661 ctcttctttg ctgtgcagcg ccagctcatt tttcatcatc aggccagaga gtgacagccc
     3721 ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag gaacttcaac
     3781 cggtgcgcta ttctgctggc caaaatgctc ttcatcaacg ataaagccac cgccaataga
     3841 gtagtaagtc tggctgtaaa cgactttatc gcccgccagc gcggtaatgc gcataccgtt
     3901 ttcatgcaga gaaaggttgt ctgcatggaa gttcatgcac tgatcaaccg ggaactccac
     3961 ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct gaataaaacc
     4021 agggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca taataatggc
     4081 gatatcagtg tggtggcctt taccggtcag agagagcgag ccgtacacgt caaccaccac
     4141 gcgggtcacg tctttaagca gattacgggc aatcagatcg tcggtaaatt gtttacccgc
     4201 tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga aaatatcgaa
     4261 tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc gaaagcggcg
     4321 cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca gacccatcac
     4381 gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta ctttctgaat
     4441 tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca gggtttcaat
     4501 catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca gcatgaacag
     4561 cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac gcagagattt
     4621 aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga aggatttggt
     4681 gatagcgata atcgcgataa tcggagccat ccacgcgata accggtgcgt taaagtggtt
     4741 agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt ctgccggagt
     4801 caggctcagt acacagctga agacgaagaa cattacggtc agcaccatca tgatgtgtgc
     4861 gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt cacgcttcgc
     4921 aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta ccggaattgc
     4981 cagccacagg gtcatccaca gaccgtttcc ggttgcagat gcagtgtcca gagacagcgt
     5041 ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca tcaatacgcc
     5101 aacaaacggg aacaccagaa tactcatcgc tttaacgatc atctgctcac cgaagcgaac
     5161 gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg gtggcgtcat
     5221 acccagctgg tgagacatga agctttcaac tgtattggtg attgccacgc tataaaccag
     5281 caggatcggg tagatagcga agaagtagag cagggtaatc agtttacctg cgccaatacc
     5341 aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag acagtacgaa
     5401 gcgagtcagg ccgcggtgag caaaaaacgt catcgggaac gcaaggatag ccatgatgat
     5461 cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc ccgcgccgat
     5521 tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg cactacgaga
     5581 gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct ggaggaatgc
     5641 aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa aaatgaaatc
     5701 tgtctatcgg cgatggctta atcatttacc gccgtaataa tttgagggcg gaaagataca
     5761 cacaagttat gtatctatca gtgatcttga tc
//
LOCUS       DAZFAH010000248         5777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFAH010000248 DAZFAH010000000
VERSION     DAZFAH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.250"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(181..900)
                     /gene="dcuR"
                     /locus_tag="ACHJWB_004384"
     CDS             complement(181..900)
                     /gene="dcuR"
                     /locus_tag="ACHJWB_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4315900.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(897..2528)
                     /locus_tag="ACHJWB_004385"
     CDS             complement(897..2528)
                     /locus_tag="ACHJWB_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4315901.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSCSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2709..2939
                     /gene="yjdI"
                     /locus_tag="ACHJWB_004386"
     CDS             2709..2939
                     /gene="yjdI"
                     /locus_tag="ACHJWB_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4315902.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2951..3223
                     /locus_tag="ACHJWB_004387"
     CDS             2951..3223
                     /locus_tag="ACHJWB_004387"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4315903.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3450..3746
                     /gene="ghoS"
                     /locus_tag="ACHJWB_004388"
     CDS             3450..3746
                     /gene="ghoS"
                     /locus_tag="ACHJWB_004388"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4315904.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3774..3947
                     /gene="ghoT"
                     /locus_tag="ACHJWB_004389"
     CDS             3774..3947
                     /gene="ghoT"
                     /locus_tag="ACHJWB_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4315905.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4066..5583)
                     /gene="lysS"
                     /locus_tag="ACHJWB_004390"
     CDS             complement(4066..5583)
                     /gene="lysS"
                     /locus_tag="ACHJWB_004390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4315906.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         1521 a         1315 c         1350 g         1591 t
ORIGIN      
        1 cttttgttag cgttttgaaa ttaaaaacac cgttcacctg aagagatatt aatttttagc
       61 gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa ggatgtaact
      121 tgtgccaggg gtattttgca ttacggtaat aattattact tacagataac cagcgatccg
      181 ttattggcaa tattgtttca gcagtgagta gtgttctgcc tgaatacggt aacggtaaac
      241 cggacgcccc gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca
      301 gatgaggtat tttcggcagg aaacacgcga gatgttaacc tcgttagcca gctcgtcggt
      361 tgaaaactca tagtcctgat gcgcgtcaat ccactggcac agcgtgcgta acgtctgcgg
      421 cgttaagcct tttggcaagc gacgaggatc ctgttcgttg gagctgctgc cgtggattag
      481 ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg
      541 ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac
      601 gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat
      661 cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt
      721 ttgcatatag atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc
      781 tttggctttc tccagcgtcg aggctgttcc acagcattga aagcctggga tttgtgctac
      841 gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat
      901 ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt
      961 tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa
     1021 ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg
     1081 tcgggtgcga tccccggtcc atcatcgtta acttcgcagt gcagccagcc gtgacggtag
     1141 tgcaatgtta cgctaatttc gccaccgggt tccggcccta atgcttccag cgcgttttct
     1201 atcagatttc ccaatgtggt aatcagcgtc gcgacctggt cctcactgcc gctgtctggc
     1261 agcaggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatcttg
     1321 ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct
     1381 tcctgatagt tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga
     1441 tgcaataatc cgagaatcac atgcaattta ttcataaatt cgtgggatcg ttcacgaagt
     1501 gcatcagcat agttgaccag accgtcgagt cgctgcatca gtttacgaac ttcagttttg
     1561 tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg
     1621 atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc ggcgcggcgt accgtcgcgt
     1681 aacacttccg agacatctac cacctgtgag catgagtggc ttagcgtcga cagtttctca
     1741 tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgacc
     1801 tcgccgcgat cgtccacggc aacgacgcct tctttgatag actgcaacat ggcctggcgc
     1861 tgctcaaaca gcgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacc
     1921 ttaaccagaa tgcaggtgcc aatcagtccg accagcatgc caaataatac cgaccagata
     1981 atgctccagc gactgtcatt gatttgttgg gtcacgcggc ttaactcaag gccgatcgcc
     2041 accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc
     2101 gccagaaaac cgcgattgat agcgacattt tctttgccat tcagcgcttt aaggatgtca
     2161 tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc
     2221 atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc
     2281 tggattccgc tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg
     2341 agggtacgcg ccactgccag tgccttgttg gccagcccgt ctcgcgtcat atcactgatt
     2401 tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt
     2461 aagatcactg tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa
     2521 tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta
     2581 attcaaaggg agtaagaatg attggctata taggggaaga gactctggca acggaaactg
     2641 ccagtgctgt atgaagattc cagggctatg cttatagcga taatcatact gatgagagag
     2701 ggaaagtcat ggatcaggcg ctactggacg agggttatcg ctgttatacc ggcgaaaaga
     2761 tcgatgtcta tttcaacact gcgatatgtc agcattctgg caattgcgta cgtggcaacg
     2821 gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgtcgtca
     2881 ctgtggttaa agtgattgat acgtgcccga gcggcgcgct gaaataccgt cataaataag
     2941 cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaggg
     3001 caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca
     3061 taccgatgtc gatgaaagcc tgaaagggca ggggattggt aaacagctgg ttgcgaaagt
     3121 cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca
     3181 cgaatttgat aaaacgcggg agtatgatga tattcgcagt tgatgggaga gtacagagtc
     3241 acgatatttt tcatgctctc cgcggagtga tgcaggagag catctgaagg gtagggggat
     3301 gcacaaagaa tgggcagaga gcgcgttttt ttgtcccaag tcatcccctt tactgagcaa
     3361 aaaaaagaat atctcctata tgagaatcat caatcggggt taataatttt tgcgtcccca
     3421 gagcattcaa tattgatagg agtcatatta tggaaggtaa aaacaagttc aatacttatg
     3481 ttgtttcttt tgattatcca tcatcttatt cctcagtgtt cttaaggtta agatcattga
     3541 tgtatgatat gaatttctct tctatcgtgg ctgatgaata tgggatacca cgacaattga
     3601 atgaaaactc cttcgcaata acgacatcgt tagccgcaag tgaaatcgaa gatttaatca
     3661 ggcttaaatg cttagactta ccggatattg attttgacct caacattatg acagttgatg
     3721 actatttccg tcagttttac aagtagccag gaaggataag aagaacataa aaaatggcac
     3781 tattctctaa aatattaatt ttttatgtga ttggtgtgaa catatccttt gtcattatct
     3841 ggtttatctc acatgagaaa acacatattc gtttacttag tgcattcctg gtcggaataa
     3901 cctggccaat gagtctgcct gtggcattac ttttttctct cttttaggag ctgtagttga
     3961 gtggacccgc tggttatcgt gaaacgaagc gtctctcctc aaaactggat ttgcggaacg
     4021 acgaacgaag cagtcgcctg actgcttcgt tcattaaagt gaaatttatt tctgtgggcg
     4081 catcgccggg aagagaataa cgtcgcgaat agtatgactg tttgtaaaca gcataatcat
     4141 tcggtcgata ccaataccca gaccagcggt tggcggcaga ccatattcca gcgcagtcac
     4201 gtaatcttcg tcatagaaca tggcttcgtc gtcacctgca gctttagcgt taacctgttc
     4261 ctggaaacgt tcagcctgat cttctgcgtc gtttaattcg ctaaaaccat taccgatttc
     4321 acgaccaccg atgaagaatt caaaacggtc ggtgatttcc gggttaacat cattacggcg
     4381 cgccagcggt gacacttctg ccggatattc cgtaataaag gttggctgaa tcagatgtgc
     4441 ttctgccact tcatcaaaga tctccgtgac aatacgtccc agcccccagc ttttctctac
     4501 cgtaattccg atagattcag ctaatgcttt agcagcatca aaattatcca ggtcggccat
     4561 atcggtttct ggacgatatt ttttgattgc ttcgcgcatg gtgagttttt caaacggttt
     4621 gccgaaatca aacacatgct cgccataagt gactttagtg gtacccagaa cctcttgtgc
     4681 cagggtgcgg aacagggact ctgtcagttc aatcaaatcg tggtaatccg catacgccat
     4741 gtagagttcc atcattgtga actcaggatt atggcgaaca gaaatacctt cattacggaa
     4801 gttacggttg atttcgaata cccgttcaaa accgcctaca accagacgtt tcagatacag
     4861 ctccggtgcg atacgcagat acatatctaa atccagagca ttatgatggg taataaacgg
     4921 gcgagcagat gccccacctg gaattacctg catcatcggg gtttctactt ccataaagcc
     4981 gcgcgcgacc atgaactgac ggatagcggc cagaattttt gaacggacaa cgaacgtttg
     5041 acgggattta tcgttagcga tgaggtccag ataacgttga cgataacgga cttcctgatc
     5101 ctgcagacca tggaatttat ctggtaaagg acgtagtgct tttgtcagca ggcgcaactc
     5161 agtacagtga atggaaagct caccggtttg cgtcttaaac agcgtaccgc gggcaccgat
     5221 aatgtcaccc agatcccatt ttttaaactg atcgttataa acaccttctg gcaggctatc
     5281 tcttgcaacg tacagttgaa tacggccacc gacatcctgc aacgttacaa aggaggcttt
     5341 ccccatgata cgacgggtca tcattcggcc agcaaccgag acttcaatgt ttaaggattc
     5401 cagttcctgg ttattcttcg catcaaactc ttcgtgcaac tggtcagagg tatggtcgcg
     5461 gcgaaaatca ttgggaaacg ccacaccttg ctgacgtagt gctgccagtt tttcgcggcg
     5521 atttctcagt tcatcgttaa aatcaatagc ctcattggct ccccgtgttt cttgttcaga
     5581 cattttggtt cctctaaatc cagctttcaa tttagcgtag ataaagagac agatcggtac
     5641 actgtattat ctgcctcgac tattaataac tcagtttatc ttatcgaaac taacgcttac
     5701 ctcaagttga tatcactaat aaataaccat caaaatggtt taaatgactt attaatctcg
     5761 atttactagt gtgatta
//
LOCUS       DAZFAH010000249         5661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFAH010000249 DAZFAH010000000
VERSION     DAZFAH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.251"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..172)
                     /locus_tag="ACHJWB_004391"
                     /pseudo
     CDS             complement(14..172)
                     /locus_tag="ACHJWB_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            170..448
                     /locus_tag="ACHJWB_004392"
                     /pseudo
     CDS             170..448
                     /locus_tag="ACHJWB_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1202..5293
                     /gene="espI"
                     /locus_tag="ACHJWB_004393"
     CDS             1202..5293
                     /gene="espI"
                     /locus_tag="ACHJWB_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO4315907.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSTLPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFS
                     SLSRDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL
                     VTEVIPADIQGKDKFNNNRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTV
                     GKPYYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVT
                     LHNYGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQG
                     EKIFSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTW
                     TGAGIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQP
                     DANQKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAA
                     DYGAEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYY
                     LLKSNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNT
                     SVEIPKLAGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNVDFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AASGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINISSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATMNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QEIGFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGT
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT         1653 a         1140 c         1356 g         1512 t
ORIGIN      
        1 atagcgaaat aaattacttc agggtttcat taaagttctt tcgtaatact ttgtagtcaa
       61 acacgttatg actggcatca aaaaccacgg atttcccctg ccagcgggta atcacctcct
      121 ccggcgtttt cggttgagcc ggtctggtta cgcccatcca tgcattaatc agtttgcccg
      181 gatgttgggg cttgaaccga agagcacggc ggtgcggagt ccggagagta acggcatagc
      241 agagagcttc gtgaaaacga taaagcgtga ctacatcagt gtcatgccca aaccagacgg
      301 gttaacggca gcaaagaacc ttgcagaggc gttcgagcat tataacgaat ggcatccgca
      361 tagtgcactg ggttatcgct cgccacggga atatctacgg cagcaagcca gtaatgggtt
      421 aagtgataac aggtgtctgg aaatataggg gcaaatccac cgtttgatta tgcagtgatt
      481 aacaaaactc cgggaagatg aatgagtata cctctcctgc aatccgtttc ctgagaaaac
      541 aggtggtaac acaatattct taatatgtta ctgtttcccc acacaaccag gtttgcggtt
      601 gtgtggtgaa ctatagtaat ctcgggtaac aatccggagc tgttcctttt atactcttca
      661 gactgtgtac tcatccacgc catccgtttt acagtctcag tgtgtgaata ataaattaca
      721 ctttgaagtg aacgccagtc aggaccatgt cgtatttccg ggtggatgag catatatcag
      781 aaactacaat aactacagat aacagttttc agtaattctg tcataacttg ccggaatgta
      841 atgtttttgt gacatggttc ttatttttta tacacaggca ggttttgtct ggatggaatg
      901 gataatgaac tcttatcatt ataaggcggt aacctctttt ttatgtgatt aactttcatt
      961 attaccaatt ctgtttatcg attcagttct ggaaatacag aaaaattcag tgcaggtgta
     1021 taaatcatca ccatgcgaaa tacataagat atattaatgt ttgttttcca taggaaaaat
     1081 cttaagtatg gacaggtatt cctgatgtca tttaaaatag attcagggtg agtatccagt
     1141 actcactctt tccggacaga caatacattt taataccata taattatata gcaggaatta
     1201 tatgaataaa atatacgcgc ttaaatatag ctcccttact ggtgggctta tagctgtgtc
     1261 agaattaagt aagaaggtca caggaaaaac cggcagaaga ttaatgacgg tttccctggt
     1321 attatcagtg actctttcta ctttaccggg taaagcatca acggtcagcg cagaaatacc
     1381 atatcagact tttcgtgact ttgctgaaaa taaaggtgtg tttacccccg gagtgacagg
     1441 aattgaaata aacgacaaca atggaaataa agttggggtt cttgatgttc ccatgcttga
     1501 tttttccagt ctttctcgtg atggtcatac cacattgatt catcctggtt atgttgtatc
     1561 ggctaaacat ggtggtttac aaagtgtttc atcagcaact tttggttatg accagatata
     1621 taaaatagtt gataataacc ttgctggtat agatttttct gccccacgat taaataagct
     1681 tgttacagaa gtaattcccg cagatataca gggaaaggat aaatttaata ataaccggta
     1741 tacggctttt taccgtgcgg gcgttggctc tcaatatatc cgttatgcaa atggcacaga
     1801 taaactactg caggcttaca ctccagaaaa ggcttatctg accggcggaa cagtggggaa
     1861 accttattat actcactata acggtatgaa gatgatttca gcaaacccgg gaaatacctt
     1921 tgataaaaac cagggacctc ttgccagtta tggacagagt ggagacagtg gttcgccgtt
     1981 atatgcctgg gataacattg acaaaaaatg ggtattagct ggagttactc tgcataatta
     2041 tggagtaaaa ggtgcacgaa atgactggct cctgatacct catgacttta tcagtcaaaa
     2101 attacaggat gaccttaaac caattattgt tgcttctccg gaggagaata tcttacgctg
     2161 ggaatttgat cgttccagag gtacaggtac tctcagtcag ggagagaaga ttttttccat
     2221 gactggtagt gtaaacggaa atgcaaatac cgggaacaat cttgtgttct caggtaatga
     2281 agggaaaatc gaactggtat ccagtgtgga acagggagcc ggatatcttc agtttgataa
     2341 agactacact gtactgacaa ataataacag tacatggact ggtgccggaa ttattgtcgg
     2401 tgacgaggca aatgtcaaat ggggagttaa tggcattgcc ggtgataatc tgcataaagt
     2461 tggttccggg acattaactg ttaatggtca tggtgagaat aaaggtggcc ttaaagttgg
     2521 ggacggcgtt gttgttcttg agcaacagcc agatgcaaac cagaaacaac aggcgtttag
     2581 ccatatcaat atagccagtg gtcgggcaac agttaaactt aacggggcaa accaggtaga
     2641 tgcagataat atcagctggg gatatcgggg tgggaaactg gatttaaatg ggtatgattt
     2701 taccttttcc cgtcttcagg ctgcagatta tggtgctgaa atcagcaacg ataatcagac
     2761 agataaatcc atagtcacac tttcgttatc tcctctgaaa gcagaagaaa taaatgtggt
     2821 tgttaataat ataaatataa tgggggggac aggcaaacca ggtgatctgt attatacgac
     2881 ctttgacgga aattattatc tgttgaaaag taaccgatat ggcagcgctt tgtttggcgc
     2941 gctgaataat cagagcgaat ggcaaaggct gggtaaggat aaagaaaaag caattgggtt
     3001 atatactcag atgaaaatgc aggaaagcgc tcctttatca tatatatatc atggaaaaat
     3061 aaccggtaat accagtgtgg aaatccccaa actggcaggc aatgatattt taacgcttga
     3121 tggctctgtc agtatatcag gagatatgtc aaaacaggac ggtgctctta tcttccaggg
     3181 acaccctgtt attcatgcag ggcaaactgt ttctgcatcg cagagtgact gggagaacag
     3241 ggagttctca ctcaacaatc tgaatcttaa taatgtggac ttcagtctgt cccgtaatgc
     3301 atttatgaac gggaatatca gggccgttaa ccagagcact gttattatcg gcggagatac
     3361 agtctttact gataaaaatg acggaacagg taatgatgtc atcagtgttg aagggaaatc
     3421 tgctgcctca ggaacatcct cctatacagg gcatatcact ctggagcaaa aatcagcact
     3481 ggatatccgc gataattttc gtggcggggt tacgtctgaa gacagtcata tcaatatttc
     3541 ttcatcttca gtcctgttct cagatgcatc gtcatttata aacagctccc tgaatattca
     3601 taaaggaggt gcgctgaccg ctcagggagg gctgtttaca agtggaagca ttgatattgg
     3661 tgacgcttcc cttctgctta ccggtacacc agtgaattca gatgatgctg cttttttacc
     3721 gaccatcaat atggctgatg gcggatttaa actgatgtct gattcatcag tactgaaagc
     3781 cagagaccag gcatctgttg ttggtgatat tatttctgat aaacaggcca caatcagctt
     3841 cggaactgaa tcaggtaaag agggcatatt atctgagaag gcatcccggg gactcgcggt
     3901 aggattactg agtggtttta atacggcata ccgcggtgca attcatgccc cgtcagcatc
     3961 tgccactatg aacaacacct ggtggcaact gacaggagac tcctcacttc gctcgttaaa
     4021 aaataccgga agcatgacat attttacagg aagtgcagcg aataaagcat tccatacact
     4081 gacggttgat gagctgacga cgaatggcac tgcgtatgcc atgcgtacgg acctgaaaaa
     4141 tgcggataag ctggtagtaa accaaaagct gtcaggtaag gataatattc tgctggttga
     4201 ttttctgaac aaacccaccg gagaaaaact ggatattgaa ctggtgagtg caccggggaa
     4261 cagcagtaag gatgttttta aaggaagtga acaggaaata ggtttcagca atgtcacacc
     4321 tgttattaca gctatagacg ccggagataa aacaacatgg aacctgaccg ggtacaggat
     4381 ggcagaaaat cctgccgcaa cccaaagtgc ctcaggcctt gcatctgtgg ggtacaaatc
     4441 atttttgagt gaggtcaaca acctgaataa acgtatgggt gacctgcgtg acatcaatgg
     4501 tgaagctggc gcatgggcac gtatcatgag cggaaccggc tctgccggtg gtggtttcag
     4561 tgacaaccac acacatgttc aggtcggtgt cgacaaaaaa catgagctgg acggactgga
     4621 tttgtttacc ggcttcactg tcacacacac tgacagcagt gcctccgccg atgttttcag
     4681 tggtaaaacg aagtctgtgg gggctggcct gtatgcttcc gccatgtttg attccggtgc
     4741 ctatatcgac ctgattggta agtatgttca tcatgataat gagtacaccg caacctttgc
     4801 cggactcgga acccgtgatt acagtacgca ttcatggtat gccggtgctg aagcaggcta
     4861 ccgctgtcat gtcactgagg atacctggat tgagccacag gcagaactgg tttacggtgc
     4921 tgtatccggt aaacagtttg catggaagga ccaggggatg catctgtcca tgaaggacag
     4981 ggactacaat ccgctgattg gtcgtaccgg tgtggatgtg ggtaaatcct tctcaggtaa
     5041 ggactggaaa gtgacagccc gtgccggtct gggctaccag ttcgacctgc tggctaacgg
     5101 cgaaaccgta ttgcgggacg catcaggtga aaaacgtatc aaaggtgaaa aagacagccg
     5161 tatgctgatg tccgttggcc tgaatgcaga aatcagggac aacgtccgct ttggactgga
     5221 gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gctgtcaacg ctaacttccg
     5281 ttactcgttc tgatactccg tattcaaaag ggcctgaggg ccctttatgc tcaggacctg
     5341 ccgcccaggt atacatttcc ggggtatcag tcgatttgtt tacttaaccg ccatgaccaa
     5401 agccggcagc aatgtcggct ttttacgtcc atatattcca ctcatccgga actgtgacac
     5461 agaacctgaa tacaggattg atcaccggtg cccacaaatg gcacattttc agtaagattc
     5521 tgaatgttac attccccctt catgtgtgac acagctgtca cagagaacga aacggagtaa
     5581 gcgtacagcc tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa
     5641 ataagtagtg ggaaccaaag t
//
LOCUS       DAZFAH010000250         5645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFAH010000250 DAZFAH010000000
VERSION     DAZFAH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.252"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..627
                     /locus_tag="ACHJWB_004394"
     CDS             <1..627
                     /locus_tag="ACHJWB_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4315908.1"
                     /translation="GHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADD
                     RALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDA
                     VFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELCTESVRGQA
                     REQFTGAVDRIFDTVESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
     gene            999..3848
                     /gene="cah"
                     /locus_tag="ACHJWB_004395"
     CDS             999..3848
                     /gene="cah"
                     /locus_tag="ACHJWB_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO4315909.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLSHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            3959..>5645
                     /locus_tag="ACHJWB_004396"
     CDS             3959..>5645
                     /locus_tag="ACHJWB_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO4315910.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1405 a         1432 c         1644 g         1164 t
ORIGIN      
        1 ggtcacagca tggttatcac tgacctgccc ggggtgggcg agagccggga cagggatgca
       61 gagtatgaag ccctgtaccg tgacattctg cctgaactgg acttggtact gtggctgatt
      121 aaagccgatg accgtgccct gtctgtggat gagtatttct ggcgacacat actgcaccgg
      181 ggacatcagc aggtgctgtt tgtggtgacg caggccgaca aaacagagcc ctgccatgaa
      241 tgggatatgg caggtattca gccttctccc gcacaggaac agaacattcg cgaaaaaaca
      301 gatgcggtat tccggctgtt ccggcccgta catccggttg ttgccgtatc ggcccgcacc
      361 ggctgggaac tggatacgct ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc
      421 agtcccctga tgacccgcct gcaggacgag ctgtgcacgg aatctgtccg ggggcaggcc
      481 cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca cggtggagag cgtctgtgtt
      541 gcctctgttg cacgcacagt tctgcgtgcc gtccgtgaca cggtggtctc tgttgcccgc
      601 gcggtatgga actggatttt cttctgaaca gtctgccagg ggaaatgtct gattacattg
      661 cgttaaccaa ctgaaaacat gatacaaaca cctcatgccc ctcctgtttc tcttgcgtga
      721 ctgccctact gttaatagaa taaaacgatc gataaaacag gtatcgatcg tttatatcga
      781 tcgtttatag tggttaatgg cctgcgaaaa ggcagctgaa tctcttcatc atgcagaacg
      841 gaattgcaca caacagactg atacttctct gtctgatgaa gggggagagc gtttacgccc
      901 gggacgtatc gctgtgcccg ataactctgt tttgtacctg ccggtatcca cttttgtggg
      961 taccggcttt tttattcacc ctctgtaagg aaaagctgat gaaacgacat ctgaacacca
     1021 gctacaggct ggtatggaat cacattacgg gcaccctggt ggtggcctcc gaactggccc
     1081 gctcacgggg aaaacgcgcc ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag
     1141 tcccggcact ggctgctgac aaggttgtac aggcgggaga aaccgtgaac gatggaacac
     1201 tgacaaatca tgacaaccag attgtcttcg gtacggccaa cggaatgacc atcagtaccg
     1261 ggctggaact ggggccggac agtgaagaaa acaccggtgg gcaatggata cagaatggcg
     1321 ggatagccgg aaacaccact gtcaccacaa atggtcgtca ggtcgtgctg gaggggggaa
     1381 cagccagtga tacggttatt cgtgacggcg ggggacagag cctgaacgga ctggcggtga
     1441 acaccacact gaataacaga ggcgagcagt gggtgcatga gggcggggtt gccaccggta
     1501 caattatcaa ccgcgacggt taccagagcg ttaaaagtgg cgggctggca acaggaacca
     1561 tcatcaacac cggcgcagaa ggcggccctg attctgacaa ctcgtatacg ggtcagaagg
     1621 tccagggaac agcagaatcc accaccatca acaaaaatgg acggcagatt atcttatttt
     1681 ccgggctagc ccgtgacact ctcatttacg caggtggtga ccagtcggta cacggaaggg
     1741 ccctgaatac cacactgaat ggcggttacc agtatgtgca cagggacgga cttgcgctga
     1801 acacggtaat taacgagggg ggctggcagg ttgttaaggc aggtggcgct gccggtaaca
     1861 ccaccataaa tcagaacggt gaactgaggg tacatgccgg cggggaagcc actgcagtca
     1921 cccagaacac gggcggtgca ctggttacca gtactgctgc aactgtcatc ggcacaaacc
     1981 gtctggggaa tttcacggtg gaaaacggta aggctgacgg tgttgttctg gaatccggcg
     2041 gtcgtctgga tgtactggag agccattcag cacagaatac cctagtggat gacggcggta
     2101 ccctggcagt gtctgccggc ggtaaggcga caagtgtcac cataacatcc ggtggtgccc
     2161 tgattgcaga cagtggtgcc actgttgagg ggaccaatgc cagcggtaag ttcagtattg
     2221 atggcacatc cggtcaggcc agcggcctgc tgctggaaaa tggcggcagc tttacggtta
     2281 atgccggggg acaggctggc aacaccactg tcggacatcg tggaacactg acgctggctg
     2341 ccgggggaag tctgagtggc agaacacagc tcagtaaagg cgccagtatg gtactgaatg
     2401 gtgatgtggt cagtaccggc gatattgtta acgcagggga gattcgcttt gataatcaga
     2461 cgacaccgaa tgccgcgctg agccgtgctg ttgcaaaaag taactccccg gtaacgttcc
     2521 ataaactgac caccacgaac ctcaccggcc agggcggcac catcaatatg cgtgttcgcc
     2581 ttgatggcag caatgcctct gaccagctgg tgattaatgg tggtcaggca accggcaaaa
     2641 cctggcttgc gtttacaaat gtcggaaaca gcaacctcgg ggtggcaacc accggacagg
     2701 gtatccgggt tgtggatgca cagaatggcg ccaccacaga agaaggtgcg tttgccctga
     2761 gtcgcccgct tcaggccggc gcctttaact acaccctgaa ccgtgacagc gatgaagact
     2821 ggtacctgcg cagtgaaaat gcttatcgtg ctgaagtccc cctgtataca tccatgttga
     2881 cacaggcaat ggactatgac cggattctgg caggctcccg cagccatcag accggtgtaa
     2941 acggtgaaaa taacagcgtc cgtctcagca ttcagggcgg tcatctcagt cacgataaca
     3001 acggcggtat tgcccgtgga gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc
     3061 tggagggtga cctgctcaga acagaggttg ccggtatgtc tctgacgaca ggggtgtatg
     3121 gtgctgcagg ccattcttcc gttgatgtta aggatgatga cggttcccgc gccggcacgg
     3181 tccgggatga tgccggcagt ctgggcggat acctgaatct ggtacacaca tcctccggcc
     3241 tgtgggctga cattgtggcc cagggaaccc gtcacagcat gaaagcgtca tcggacaata
     3301 acgacttccg cgcccggggc tggggctggc tgggctcact ggaaaccggt ctgcccttca
     3361 gtatcactga caatctgatg ctggagccac aactgcagta cacctggcag ggactctccc
     3421 tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt gcacaacatg
     3481 tgcgtgccgg tttccgtctg ggcagccaca acgatatgac ctttggtgaa ggcacctcat
     3541 cccgtgacac cctgcgcgac agtgcaaaac acagtgtgag tgaactgccg gtgaactggt
     3601 gggtacagcc ttctgttatc cgcaccttca gctcccgggg tgacatgagc atggggacag
     3661 ccgcagccgg cagtaacatg acgttctcac cgtcccggaa tggcacgtca ctggacctgc
     3721 aggccggact ggaagcccgt atccgggaaa atatcaccct gggcgttcag gccggttatg
     3781 cccacagcgt cagcggcagc agcgctgaag gctataacgg tcaggctacg ctgaatatga
     3841 ctttctgatt atacggtatc ccggaaatac gatttcagga aaattcatgc agtaatggga
     3901 aaggacgctc attgctgttc accgcagttc tgaattattg aatttataaa ggtgaatgat
     3961 gcacgaaaag aacatcgccc tgctttgtga tgaagccgac cggcttttgc aactgaatat
     4021 caatctgctc cggcaaatgg ttgatgagcc ggatgtgtta tctgacagta agaacgaaaa
     4081 cgggctgctt tttgataaac ggaaagcgct gaaaagaatt gaggagctgg agggcgaaca
     4141 aatcaaaacc gcccgcaggg agatggtgct ggctgtggtc ggcacgatga aggcgggcaa
     4201 atcaaccacc atcaacgcca ttgtggggca ggaaattctg cccaaccgta accgccccat
     4261 gacctcggta ccgacattga tccgccacgt tcccggaaaa acggaaccgg tcctccatct
     4321 ggaacatatt cagcccgtcc gcaatttatt aatcacactg caggaaaaac tcgccacccc
     4381 ggcagggcag caagtcgcac agtccctgca gcaaaccggg gatacccgcg aactactgga
     4441 tattctggcg gatgatgtct ggcttaaaaa tgaataccac ggggaagacg aaatctttac
     4501 cggactggca tcgttaaatg atctggttcg tcttgctgcg gcaatgggga ctgaatttcc
     4561 ttttgacgaa tacgcagaag tgcagaaact accggtgatc gacgtggcgt tcagccacct
     4621 ggtgggaatg gatgcatgtc agggaacact cacactgctg gatacccccg gccctaatga
     4681 agccggacaa ccacagatgg aaatgatgat gcgggatcaa ctacagaaag cctctgcggt
     4741 gctggcggtg atggattaca cccagatgaa ctcgaaagcc gatgaggagg ttcgtaaaga
     4801 gcttaatgcc attgctgacg tctcagccgg tcgtctgttt gtgctggtca ataaattcga
     4861 tgaaaaagat cgcaatggtg atggtgcgga tgccgtacgc cagaaggtgc cagcaatgct
     4921 gaacagcgat gtgctgcccg cctcccgcgt ttatcccggc tcatcacgtc aggcgtacct
     4981 ggctaaccgt gcgcttcatg agttacgaaa aaacggtgcg cttccggttg atgaagcctg
     5041 ggtcgatgat tttatcaggg aagctttcgg acctatggtg gaagaggacg actggaaaga
     5101 cagcactaag gtaaataaaa aggcagaaaa actctggaat atatctctga tcgatcaact
     5161 gataacggaa gtcatacaga gctcccattc cagagcggcg gcactggcgg ttgactctgc
     5221 cgccgcaaaa ctgatgcaga atgcagaaaa tgtcagcgaa tatctgtcat tacgtcatca
     5281 ggggctgcag caaagcattc agtcactaca ggcgcatatt accagcctgc tggcggatat
     5341 ccaggagatt gaggaatgtc agaatcaggt gaccggtgat gtcagaatgg ccatggagga
     5401 catcaatacc aaaaccgggg aattactgac gaaggtctgt gcctcactgg aggaggagct
     5461 gaacgactat ttcagaagcg gtaaacgcaa agaacagcaa atgctggagg aagaaaattc
     5521 agcgcagccc cgggagagaa acgcattcgc tttctttcac gatattttcg gaaccggaaa
     5581 tcagcatgac aggatgcggg actttgatcc ggacagcccc gaaataaaat tcagtgatcg
     5641 caggg
//
LOCUS       DAZFAH010000251         5629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFAH010000251 DAZFAH010000000
VERSION     DAZFAH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.253"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(200..756)
                     /locus_tag="ACHJWB_004397"
                     /pseudo
     CDS             complement(200..756)
                     /locus_tag="ACHJWB_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            877..987
                     /locus_tag="ACHJWB_004398"
                     /pseudo
     CDS             877..987
                     /locus_tag="ACHJWB_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1064..2029)
                     /locus_tag="ACHJWB_004399"
                     /pseudo
     CDS             complement(1064..2029)
                     /locus_tag="ACHJWB_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2096..2809
                     /locus_tag="ACHJWB_004400"
                     /pseudo
     CDS             2096..2809
                     /locus_tag="ACHJWB_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            2938..3318
                     /locus_tag="ACHJWB_004401"
     CDS             2938..3318
                     /locus_tag="ACHJWB_004401"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4315911.1"
                     /translation="MQKNVTLGRRKGCPNYPPEFKQQLVAASCEPRISISKLALENGI
                     NANLLFKWQQQWREGKLLLLSPESPQLLPVTLDATAEQPESPAESPAPLSISCEVTFR
                     YGTLRLNGNVCEKRQALLIQELKR"
     gene            3315..3652
                     /gene="tnpB"
                     /locus_tag="ACHJWB_004402"
                     /pseudo
     CDS             3315..3652
                     /gene="tnpB"
                     /locus_tag="ACHJWB_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            3723..5232
                     /locus_tag="ACHJWB_004403"
                     /pseudo
     CDS             3723..5232
                     /locus_tag="ACHJWB_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060488.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            5283..5468
                     /locus_tag="ACHJWB_004404"
                     /pseudo
     CDS             5283..5468
                     /locus_tag="ACHJWB_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1446 a         1456 c         1371 g         1356 t
ORIGIN      
        1 atatacgaaa atacagaaat gtgtatttat tgttcattaa tatactttca aatcgtcgtg
       61 gaacaatgat ccaacccacg taatttggac acaggtctaa gcgaggctct ggttttcaaa
      121 ttattccgaa ctgaggtcgc cacaccagct gtgccatcat ctgtgatggc attgaccagc
      181 caataacgtc acgcggtcat aggtctatta ccaccgctag tacagccagc tttcatcagt
      241 acgcagatac gtgatattac cctcccactt cggttctgag ccttggtgta aaaatcatgc
      301 tccagcagat tttctggcac aggcagatcg tgaacactgt agctaactgg gctgaacttt
      361 cgggaagctt ttgccctcat ccctaacggc acaagcttac tgctactgtt attactgttc
      421 aagtggtaac tctgagcaca caattcatta ctcaagcgtg aagcaccgta acactggacc
      481 aagtaaaaac ctcgcagaca ctgatgtcac agtgcttacg gaactgctgg cgtgtgctta
      541 tctttgttcg ccgctgacac cacggatatc aggcgctgtg accatccgga agcttcgatg
      601 ctaaactcat ccagatattt ttcaataaaa gatagtctgc tttttatccc attctgctaa
      661 ctggcgtttg agacgtgcaa tctcaataga catctccaac tcaccttcag aggaaatctg
      721 ctgattttgc tatttactgc gccagttgca gagttttcag gacttcactg cgaaattcaa
      781 atgaatactg gtaagattgt aagtatcccg cataatcgtg ccattcacac ttagagatca
      841 tccggcataa tcagtctgtc aacgaaggag atcgctatgt gtaaagcccg ttttactgag
      901 catcagatca tcgctgtgat taagtcggtt gaagccggac gagacgttga agatgtcggc
      961 cgggaggctg gtatctctga ggccaatata atagtcaggt tccgattgtg caggcgcaac
     1021 ctgtcactat ttttcagtac actgcaactc gtctggctgg gtactaactt ccaactgcct
     1081 gatactcggt atcatttctc agcatcgacc agattattct cgcgttcttg ttagcgaccg
     1141 ccacggtcgc tttattaaac cagcgccgtt cctttaacta gttaacccac tgatgcaagc
     1201 agccaattgt tattcatatc aactctgacg acagcgcggg caccatgaat aaaaaatgta
     1261 cgcaaatgct tgtcgccttt tttcgtcata ttcattagta cctgcttgtt gccactagag
     1321 tgctgtcgtg gaaccaggcc aagccatgcg gcaaagtgac gaccattttt aaattcagtt
     1381 cctttggcaa tgctctggca ggcttctgat tgcatgaaca cagcgtcaat ttctttatca
     1441 aaaaagttta tacggcggcc aagatcgtta aagagatcat aaagctctgc aaccgttctg
     1501 cgcatacggg cacttaaccc gttttctgca tcctcaagga taagcagaac ggcacggcga
     1561 actcttgaga ctgccgcacc aattgttatt ccccgatcaa gcagcagctc tcggatttga
     1621 cagactatcg cagtacgatg attgacaata cgctgtcttg cacggtgtag cccctcaata
     1681 tcctgccgtt cagggctttt cggtggaaca aactgcatcg taggttgcat cagagccaca
     1741 gctatcgcct gcgcatcgtt accgtcattt tttgcccacg gacaaaaggt tttacatact
     1801 gggggctgat gacttttaca ttatgcccca gtttctcaaa ctcacgctgc cagtaaaatg
     1861 cacctgtaga tgcttctata ccggtcagac acgccggaat attcgacacc gtctggagta
     1921 actcttttcg gccggtacgt tttgaataaa acggtttacc tgtctagttt aacccgcaga
     1981 gctgaaagac atttttagcc agatcaatac ccagaaatac gatattcata gtgattctcc
     2041 tgctgagcac atttcgtaca gttaaccgca gaaggaggag gtagtccatc ccattaactg
     2101 gaagtctaga tacggcggta tggcatcttc tgatattaaa aagatcaagg atcttgagga
     2161 cgataacagg catcttaagc agatgtttgc cgatctcagt cctgagaacc gggcgctgaa
     2221 agatgttatc gaaaaaagct ttaaaaccag cctttaagcg tgagctggtc actcatctga
     2281 taacgacgtt cggactcagt attcatcaag ccagccagag tctcaatctg agcagaacgg
     2341 tttaccatta ccgcccggat atcacgcgtg atgagcccgt tattgccgca ttgcaggcgg
     2401 aggctaaacg cccacgacat ggttttccta aactttacct agttctgcgg cggcagggat
     2461 atccgtggaa tcacaaaagg ctccaccgta tttagtgtct gctgaaactg aattttcgcc
     2521 gtaagggcaa acagcggttg ccggtacgta atccatcacc attggccacg ctgtcagcgc
     2581 tgaaccagag ctggtctgtt gattttatgc atgatgctct ggcctgtggt cgtcgttttc
     2641 gtacgttcaa tatcgttgat gactttaacc gtgaggcgtt gtcgattgaa atcgatctga
     2701 atctgccagc tcagcgagcg attcgtattc tcgacaggat cgcggcaaac cgtggcgcta
     2761 tccggtcatg ctgcgcatgg ataatggtcc ggaatttatc tcactggcgg cagactgaat
     2821 ggacagagag gcacacagta aaactgtaag cgtacagcct gaaccgtctg gtcagaatct
     2881 gacgaattag acaaagttgt gtccacaaaa taagtagtgg gaaccaaagt gtcagatatg
     2941 cagaaaaatg tgactctcgg caggcgaaaa ggctgcccta attatcctcc cgaatttaaa
     3001 cagcagctcg ttgctgcctc ctgtgaacca aggatatcca tctcaaaact tgctcttgaa
     3061 aatggcatta acgccaatct gttgttcaaa tggcaacaac aatggcgcga gggaaagctg
     3121 ctattacttt ctccagagag ccctcagcta cttcctgtga ctctcgatgc aacagccgaa
     3181 cagccagaat cgcccgctga gtccccggcc cccctcagta tcagctgcga ggtaacgttc
     3241 cggtacggga cgctccgcct caatggcaat gtctgcgaaa agcgccaggc tctgctgata
     3301 caggaactga agcgatgatc ccgttacctt cctggaccaa aatctggctg gttgccggta
     3361 tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa ggtacagact acgctgaaag
     3421 acgctcccat gccacccatg ttttcatttt ccggggccgc agcggcagtc aagttaaact
     3481 gctgtggtcc accggtgacg ggctgtgcct cctgaccaaa cggcttgagc gtgggcgctt
     3541 cgcctggccg ccaacccgtg atggcaaagt gttcctcacg caggcgcagc tgttggaagg
     3601 tatcgactgg cgacagccta agcggctgtt gacctccctg accatgctgt aaatctcttt
     3661 atcctggttg tcacagaata agcccggtaa aatacgggtt tatgacgaca tagtctgacg
     3721 acatcttcct gctgaaacag cgcctagccg aacaagaaac gctgacccag gccctacagg
     3781 aaaagctgag caaccgggtg cgcgaaatag accatctgca ggcgcaactg gataaactcc
     3841 gccggatgac cttcggcaat cgttccgaaa aagtctcccg ccgtatcgca taaatggaag
     3901 ccgatctgaa ccagttgcag aaagagagcg atactctgac cgttagggtg tatgacccgg
     3961 cagtacagcg tccgttgcgt cagagccgcc ctagtaagcc gtttcctgaa tcactacccc
     4021 gcgacgaaaa gcgactgttg cctgcgacgc cgtgctgccc gaactgcggc ggttcacgga
     4081 actatctggg cgaagatacc gccgaacagc tggagctgat gcatagcgcc tttcgtgtta
     4141 tccggacaat acgggaaaac atgcctgtac tcagtgcgat gccatcgtgc aggcaccagc
     4201 accttcgcgg cccatcgagc ggggtatcgc cggaccgggg ctgctggccc tcgtgctgac
     4261 ctcgaagtat gcagcccccc cattgtatcg ccagtcagaa atatacgacc ggaaaggtgt
     4321 ggagctgagc cgttcactgc tgtcgggttg ggtggatgca tgctgccgac agcagtctcc
     4381 gctggaagaa gcgcttcatg gctctgtcat gactgacggc aaactccatg ccgatgatac
     4441 cccggtccag tactgctgcc gggtaataag aagacgaaga ccaggcggtt gtgggcgcat
     4501 gttcgtgatg accgcaatgc cggctcagcg ttggcacctg cagtgtggtt cgcttacagc
     4561 ccagacagaa aaggcatcca tccgcagact cgtcttgcct gcatcagcgg tgtgctgcaa
     4621 tcggatgcgt actccgggtt caacgagctg tatcgcaatg gtcggataac ggaagctgcc
     4681 tgctgagctc atgtccgccg aaagatccac gatgtgcacg ttcgcatccc gtcagcactg
     4741 acggaagaag tcctggggca gatcggtcag ttgtacgcca tagaggcgga tataagggga
     4801 atgccgacat agcagcggct tgctgaacgt cagctaaaaa cgaaaccgct gttgaaatcc
     4861 ctggaaagct ggttgcgtga aaagatgaag accctgtcgc aacacacaga tttggcaaag
     4921 gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgga cgatggctag
     4981 gtcgaagcag acaacaacat cgctgaaaat gccctgcggg cggtcagcct gggtcgtaaa
     5041 aacctcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctgct gtacagcctg
     5101 atcgggacgt gcaaactgaa cgacgtggat ccagaaaact accttcgcca tgtgcttggc
     5161 gtcatagctg actggccggt caaccgtgtc agcgaactgc ttccgtggcg catagcactg
     5221 ccagctgaat aacacatccc tgtcaatacg gccctcgctg tgcgcttacg taaaactgga
     5281 ttatccagcc aagtaagccg acgtagaatg ctttcatcga gcgttttaac aggacatacc
     5341 gtacagaaat actcgatttt tatctgttca gaacgctgaa taaagtgcgg gaaatcacgg
     5401 agaaatggtt atcagaatat aactgtgaac gcccgaatga atcactgaac aatatggtac
     5461 cagagtagta ccgacagcac cattatttag ccgagttgtc aaaaaatacc tagaactaaa
     5521 acgggcctct ttataagatc attcgacatg cagaccccat aaatagtctc aagcgttcgg
     5581 ttagcgctta tctaaataac aatctacctt tgtaaaataa ctgtatgtc
//
LOCUS       DAZFAH010000252         5609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFAH010000252 DAZFAH010000000
VERSION     DAZFAH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.254"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..967
                     /locus_tag="ACHJWB_004405"
                     /pseudo
     CDS             1..967
                     /locus_tag="ACHJWB_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1032..1994)
                     /locus_tag="ACHJWB_004406"
     CDS             complement(1032..1994)
                     /locus_tag="ACHJWB_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO4315912.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDASVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPVINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(1991..3196)
                     /locus_tag="ACHJWB_004407"
     CDS             complement(1991..3196)
                     /locus_tag="ACHJWB_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4315913.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGAVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVTEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            3567..3740
                     /locus_tag="ACHJWB_004408"
     CDS             3567..3740
                     /locus_tag="ACHJWB_004408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315914.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCVELDNDQ
                     LNEIIEWGRLYQR"
     gene            3913..4164
                     /locus_tag="ACHJWB_004409"
     CDS             3913..4164
                     /locus_tag="ACHJWB_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO4315915.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            4161..4448
                     /locus_tag="ACHJWB_004410"
     CDS             4161..4448
                     /locus_tag="ACHJWB_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4315916.1"
                     /translation="MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDKQLIIAVVAVGKRERSDVYNLASERIR"
     gene            4450..4728
                     /locus_tag="ACHJWB_004411"
                     /pseudo
     CDS             4450..4728
                     /locus_tag="ACHJWB_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5376..>5609
                     /locus_tag="ACHJWB_004412"
     CDS             5376..>5609
                     /locus_tag="ACHJWB_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4315917.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPPLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMD"
BASE COUNT         1535 a         1293 c         1322 g         1459 t
ORIGIN      
        1 gcgcgatcgt gatggctctt ttgaaccgca acttgtgaag aaaaaccaga cccggatcac
       61 cgggatggat aaccagatcc tgtcgttgta cgccaaaggc ctgacaaccc gcgaaatagc
      121 ggctgcgttt aaagagctgt atgacgctga tgtctcaccg gcgctggtat cgaaggtcac
      181 cgatgccgtt atggagcagg tagtcgaatg gtaaaaccgg ccactggatg cggtctatcc
      241 cattgtttat cttgactgta ttgtcctgaa ggttcggcag gacagtcgcg taatcaataa
      301 atccgtattc ctcgcactgg gtatcaatat cgaaggccag aaagagttac tgggtatgtg
      361 gctggccgaa aacgagggag cgaagttctg gctcaatgtg ctgactgaac taaaaaaccg
      421 cggcctgaac gatattctca tagcctgtgt cgacgggctg aaaggcttcc cggacgctat
      481 caacacggtg tatccggaag cccgcatcca gctgtgcatc gtgcatatgg tgcgcaacag
      541 cctgcggttc gtttcctgga aggactacaa agccgtcacc cgcgacctga aagccatcta
      601 ccaggccccc acagaagaag caggccagca ggcgctggaa gcgttcgcca gcgcctggga
      661 cagtcgctac ccgcagataa gccggagctg gcaggcaaac tggaccaacc tggcgacgtt
      721 cttcgcttac ccggcagata tccgcaaagt catctacaca accaacgcca tcgagtcgct
      781 gaacagcgta atccggcatg ccatcaaaaa acgcaaggtg ttcccgacgg atgactcagt
      841 gaaaaaggtc gtgtggctgg caatccaggc ggcttcacag aaatggacga tgccactgcg
      901 ggactggcgt atggcaatga gccgctttat tatcgagttc ggtgaccgcc tggacggtca
      961 cttctgagaa aaggcattta cacagaatcg tgtacagggt ctttggcatc ctgatattaa
     1021 cccggcatcc cctattcagg cagttttctt agaatgtttt ttatagcctc atcgatttct
     1081 aactgcacct cattagatat gcgggagaac tcataaacaa caagacgctt tctggggtca
     1141 gtcttctttc tggcaaattg gttacgatca gaaaactctt tcaacttctc agtttcaact
     1201 gtttttacag gccggtctgt aagcaatttt ccttcagacc tgaagaccgc cagaattttg
     1261 cttttctcaa ttgttggata gtcaggtaaa gccatcagtt tttggcgaac tctatccata
     1321 aactcttcca ctgacatttt tttgttggtt gccttttcag acagtttcaa caaaaattga
     1381 tagtccgaca gcgaaatatc attgattacc ggaaatacag agatcatttc atccggtact
     1441 gatgccgcct gaaatgcgcg tgttactttt gccggagaga tgctttcagc ctgagcgatc
     1501 tcttcttttg tcatacttgc cccataagta acttcaaggc gttttcctag ttctcggagg
     1561 ctatgctctc tggctgtctg tatatctttt gcaagctgtc tcgcgtcggc aatatcaata
     1621 ctgtcttttg taaccagtat ttcgaatttt acgttgttat agatacaggc agcgcgacga
     1681 cgcgtaccgt ctaatacttc aattcgtcca tctacttccc gaccaatagc cggaaaaaat
     1741 tgctgaagtt taatcgtgcg agttatgtca ctaactgact cacgcgaaag catagtctga
     1801 tcacgtccat taacagatgc atcaacgaac gttttagatt ctatatctcc acttaataca
     1861 accgttttaa tgaattttgc ttgtttccca gacttaagag taaacgtttt gatctctccg
     1921 tcaccttcaa gcatgcgagc gaactctgaa ttgctattgc cgagaactcg ccctcgagct
     1981 aaaacctttt tcatttggat gctccccgaa tgtattcaat gcggtcaaaa acggctttag
     2041 taaatcgttc cgcttcggta cgagcttttt tcaatgcctc tgcacttcct ggatacgaaa
     2101 cagggttagc actgatgata gtgtcaaaag attcaccaca tcgttcgaac ccgtccagtc
     2161 tcggaagcga tgagtcaaga atattactcg catacacttc acgagccagg ctatgggaag
     2221 tttcgtgatc gcgcttactg gtcattttgg acataaaccc tatgctcgca ctcaggcggg
     2281 gctcaacgcc ttcttcttcc aaacgttcta gcatttcagg taaacgagtc aaatatttga
     2341 gagttgaatg gaaatcaact tgcgccgggg gagtaggggt gagaagtaaa tcactcgcag
     2401 ccaggccatt gagaagaaat ggatcaagat gagggccagt atcaataaag acaaaatcat
     2461 aatcatcagc gatacggtca atgatggttt tcctgagtac ttcagaaggc ttaaaaccag
     2521 gaaggtgttc agtaaccaaa gactcccatt ggcttgctac gaagccgtca tcaatagaag
     2581 cagggatgac atcaacgcca ggaatcatag tgggtcggat aaccgcttct cgcagtgttt
     2641 cagcatcgag attatttagc attgcctggg cggctgtttc aaggacagca ccaatactat
     2701 gggtatggtc caaaaacata gtgctggacg cctgcgggtc aaggtcgatc acaaggatac
     2761 gaaggtcatg acgcagcaaa tccggatgta ctcgtagtgc atgcgccagc gttactgtgc
     2821 tgacggtttt tgaaacccca cctttcaagt tgacgacaaa aattacgtaa ggttctttat
     2881 gaacatcgcg atattttggt atctgtcggt gagcgtaaat atcaataacg ttctgaattg
     2941 ttaatgcata ttgctcgacg ttgcctaccc gctttttatt aaattcatag ccactttctt
     3001 ccatttcttt aacggccagt tctacgatgc gtcggctcag cttcggcaat tttgcgaccg
     3061 cattacgagt atatgtttga tgatactctg tcagattgaa ttcttttctt tgctcctcaa
     3121 tatcccgact catggcctga agtagagcac ttgctctgag cgctatggta ccgacactac
     3181 cgtaatctcg tttcatagtt ttcctctgag atagattccc tgcccatata ttgcgtacaa
     3241 atttcaatca cattgtacac aaacatgaaa aacgtacaat atattttgca ttgtcattaa
     3301 attattgtac attgcaagtc accattcaca tcaaaccgca tagttagaca ttgtctctga
     3361 gcaggcgagg ggttagacaa gaccgttggg cgcgttagag tgtcatatta ggatacgggt
     3421 gggtgacgcc cacctctggc atagaacgga cattcattga tgccatgcca gaatggacgt
     3481 tcaggttatt ccgtccagtt ctgctggcaa cgcgagatct ccccaggtat agtgatgcca
     3541 cggcaaagcg ctcaaacagg gataatatga tggaaatcaa ggctcaacag tttgtcacat
     3601 caacggggcg gcaagttctt actgacaacg gacaacaagg tatgggcggc gtggcggaag
     3661 cgatttttgc gaactgcgtg gaactggata acgaccagct taacgagatc atcgagtggg
     3721 gtcggctcta tcagcgctga atgccactat caggctgcgc aagcggcctt ttttacgccc
     3781 cttgtttaat tcccgcacta cctggacgtt caggtgattt tgtctatctg tacaaaaaac
     3841 aacaaaagac ttgttaacag gtcatgtaag gagtatcttt gagactggtt aaacagtctt
     3901 gaaaggtggc ctatgcctaa cattattctc agtgatacaa gcgccagtgt cagcgagctg
     3961 aagaaaaacc cgatggcgac agtcagcgcc ggtgatggtt tcccggtcgc tatcctgaac
     4021 cgtaatcagc ctgctttcta ctgtgtaccc gcagagctgt acgaaaagat gctcgatgcc
     4081 ctggacgatc aggagctggt taaactggta gccgaacgca gcaaccaacc gctgcatgat
     4141 gtagacctgg ataaatacct atgaggtatc aggtaaaatt cagggaagat gcgctgaaag
     4201 agtggcaaaa actggataag gctattcagc aacagtttgc gaaaaagcta aaaaagtgct
     4261 gtgacaatcc gcatattcct tccgcaaaac tgcgcgggat aaaggactgc tacaaaataa
     4321 aattacgtgc gtcaggtttt cgcctggtct atcaggtgat tgacaaacaa ttaattatcg
     4381 ctgttgtagc tgtgggtaaa cgtgagcgca gtgacgttta taatcttgcc agcgaaagaa
     4441 taagataaaa gcaataaata cagaaagtta ctctggcgtt atggggtaat gcaaagtatg
     4501 agtcgcaggg ggaattgcct ggataattcg ccgatggaaa gagtctttcg cagccttaaa
     4561 agtgaatggc ttctgaaagg tggttatggt gattttagcc atgcggtacg cgatataaac
     4621 cagtggatta atggttatta caacgtttat cgacctcaca cgaacaacga tggtttaccg
     4681 ccttgcttac atgaagaaaa gtggaaacag gttatcccgg tgtcctgatt ttgtgatcca
     4741 ctacaatata caatccttgg cattgaaaca aacatatgaa gtgtactttg gatatgagcg
     4801 tacgttcagg aagtacggca gattgagtcg aaaattacct gtaaagacag acatcagaaa
     4861 aaacagtgct cgccgcagac cggtgaccgg gcaaagcccg cgaaccggcg aggaagcgca
     4921 atgtgtctgt agttacaatg agcaggtacg ccctgaagcc cttataagcg gttgtgtatg
     4981 ctgtttcccc tgggatgatt tatccacata tccagaggtt cgatccagta aacagatcca
     5041 tgaatgatca acaaaggatc cattaaagat ccccatatct ctgtaaacct tgtcactcat
     5101 gggccgggac cacgatcaca taagctgtgg catgttactg ataaactgta acatgctaat
     5161 gataagctgt attcagtaat ccatatactg aagtaagtta atgacataaa ctgtggtcag
     5221 tacgccagac tcagttgtta aatacaggct gcaggttttt cttcagtcag ttagcggggc
     5281 tctgacacac gatttgctgt ttattctttt actgtccaca ggcaggaggc tttctggaaa
     5341 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcatcc ggtgagctgg
     5401 tgacactgac accaaacaat aacaacacag tacaacctgt ggcgctgatg cgtctgggtg
     5461 tctttgtacc gccccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg
     5521 atgccacgga agagctgaca cgtctttccc tggcccgtgc tgagggattt gataaggttg
     5581 agatcaccgg cccccgcctg gatatggat
//
LOCUS       DAZFAH010000253         5601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFAH010000253 DAZFAH010000000
VERSION     DAZFAH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.255"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(88..1065)
                     /gene="wzzB"
                     /locus_tag="ACHJWB_004413"
     CDS             complement(88..1065)
                     /gene="wzzB"
                     /locus_tag="ACHJWB_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4315918.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(1212..2378)
                     /gene="ugd"
                     /locus_tag="ACHJWB_004414"
     CDS             complement(1212..2378)
                     /gene="ugd"
                     /locus_tag="ACHJWB_004414"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4315919.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(2625..4031)
                     /gene="gndA"
                     /locus_tag="ACHJWB_004415"
     CDS             complement(2625..4031)
                     /gene="gndA"
                     /locus_tag="ACHJWB_004415"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4315920.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIICAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(4228..>5601)
                     /locus_tag="ACHJWB_004416"
     CDS             complement(4228..>5601)
                     /locus_tag="ACHJWB_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFO4315921.1"
                     /translation="SGARGLVIDFSHDVCAAFTHAFLSVIDDKYNFNKVALAIDNRPS
                     SYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPCIMVTGSHIPFDRNGLKF
                     YRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYYLERYVSLFNPEILKGKR
                     IGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEAVSDEDRILAREWSKKYN
                     LDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKALAIPVSCNTAIEESNKF
                     ASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASDLQINGKELKSLPTRDAV
                     LPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQQIIKNAISSPNNFFNSL
                     GYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYAEASDENQAKQYVTNVLG
                     NITSLIS"
BASE COUNT         1583 a         1278 c         1178 g         1562 t
ORIGIN      
        1 actttggttc ccactactta tttggtggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttacgatta cttcgcgttg taattacgca gagcgttacg
      121 ccccagcaca atccccgcgc caaccatgcc gcccagcagc accgccagaa tcaaggtgat
      181 tgcctttttc ggactatcgc gacgaatagg taacgtcggt ttcattacat agcggtaagc
      241 atgaatatca agatcatcaa cctttaagtt ttcgatatcc agcaggtttt gacgagtctg
      301 atagtagttt ggtgagaaca ccaacggacg ggtcgcctca tgcttaatca tcgactccag
      361 cgcttcgctc cccaaaagga acaatgtatc ttgcgtcaca tcttcagtct gctgaacctg
      421 tggctttgtc acctgcgcct gattcgcata ctgcaacgct tcctgaatct gacggatacg
      481 cagatctttc tgctcctgcg caactacttc ctgagttttc agagaatcct gcaagttttt
      541 catacgcagc gcgatgttgt ctttcaggtc tttttccagt tcttcgttta cctggtcgtc
      601 aacttgctgg atatacttcg ccagctgctt ctgcgcacct tcaggtgttt caccaacata
      661 agacaccgcc aatggtaacg actggttttt aacggtaggc tcaatggtca gcttctctgc
      721 ttcttcctgg ttatccagcg tttccgctaa tgctgagaat gcagtgctgt aacgtccgat
      781 aagcgaagcc tgaatgtcgg agactttcgg tacagccgat ccgtagatca cattcatcgc
      841 gttggtataa ccagcgattt gccccacatc aggttgagta acgatagctg tggaagtcca
      901 cttttctttg gcaacggcca gataccctat agccagggca atagcaacaa tgacagagat
      961 gataattgtc atcttgccgc gccacaactg caccagtaaa tcaatcaaat caatctgttc
     1021 cgggtcatgg ttttgcccag aaacattatt attttctact ctcatcatta ccctaacgga
     1081 cgaaataatt ttaagtcgcc atagtttagc gataatcgcc atgaatgcta taggataaat
     1141 gataaaaata atgaattatt acaaagggaa cataagagaa tcagggcgaa aatcgccctg
     1201 ataacaggat gttagtcact gccaaagaga tcgcgggtgt agactttgtc tgccacatcc
     1261 ttaagctctt ctgccatacg gttggaaata atgacgtcgg cttgttgttt gaaggtggcg
     1321 agatctcgtt ccaggcgaga gttgaagaat gagtcttctt tcatcactgg ctcgtatatg
     1381 atcacttcaa cgcctttcgc cttgatacgc ttcataatcc cctgaatgga agacgcgcgg
     1441 aagttatctg aaccgctctt cataatcaga cgataaatac ccaccacttg cggcttgcgt
     1501 gacaaaatgg catcggcaat aaaatcttta cgcgtgcggt ttgcatcgac aattgccgaa
     1561 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     1621 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatgcg tgggtcgaga
     1681 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata gctatccagt
     1741 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     1801 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     1861 aacgcagcaa aacgttctgc acgttctgaa cgctcaccga tgacaatacg tgacggatgg
     1921 agattatcgt aaagggcttt accttcacgg agaaattccg gagagaaaat aatattttca
     1981 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     2041 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     2101 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     2161 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
     2221 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
     2281 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
     2341 aagcctacat agccagtacc agaaatggtg attttcattt catgctctca gaattaactt
     2401 aactgtgaat catgatgttt ttagcatcct gataagagct aaaagtttta acgctacgtt
     2461 gtcagatggt caacgcaacg aaaaacaatt cagattttct ttataagaat agctgatatt
     2521 tattaagtta atattaagca gtgaaattta gtctataaga taattggcaa aaaaagcccg
     2581 gcgcaatata cgccaggcct caatttattg ttagttaaat aagattaatc cagccattca
     2641 gtatggaaca caccttcttt atcaatgcgc ttataagtat gtgcaccgaa atagtcacgc
     2701 tgtgcctgga tcaggttcgc aggcagaaca gcggcacggt agctatcgta ataggcaacc
     2761 gcagcagcga aggtcggaac cgggataccg ttctgtactg cataagcaac gacatcacgc
     2821 agcgcctgct ggtagtcatc ggcaatttgc ttgaagtacg gagccagcag caggttagcg
     2881 atctgcggat tttcggcata agcatcggtg attttctgca ggaactgcgc acagatgatg
     2941 cagccagcac ggaaaatttt cgcgatttcg ccgtagttca gatcccagtt gtactcttca
     3001 gacgcagcac gcagctgaga gaagccctgg gcgtaagata cgattttgcc cagatacagc
     3061 gcacgacgaa ctttttcgat gaactcagcc ttgtcgccag ctggctgtgc ttgcgggcca
     3121 gagagaactt tagatgcggc aacacgctga tctttcagag aagagatata acgtgcaaat
     3181 acagactcgg taatcagcga cagcggttcg ccgagatcca gcgcactctg gctggtccat
     3241 ttacccgtac ctttgttagc cgcttcatcc aggatcacat caaccaggta gttaccgtct
     3301 tcatcttttt tggtgaagat atctttggtg atgtcgatca ggtagctgct cagttcaccg
     3361 ttattccact cggtaaaggt ctgcgccagt tcttcgttgg tgaggttcag gccaccttta
     3421 agcagagaat aggcttcagc aatcagttgc atatcaccgt attcaatacc gttgtgaacc
     3481 atcttcacat agtgacctgc accatcggca ccaatatagg taacgcatgg ctcaccgtct
     3541 tcagccactg cggcgatttt ggtcaggatc ggcgcaacaa gttcataggc ttctttctgc
     3601 ccaccaggca taatggaagg acctttcagc gcaccttctt caccgccgga aacaccggta
     3661 ccaatgaagt taaaaccttc ggcagaaagc tcacgattac gacgaatggt gtcctggaag
     3721 aaggtattac caccatcaat gataatgtca cctttatcga ggtatggctt gagggaatca
     3781 atagcagcat ccgtgcctgc acctgctttc accattaaca ggatgcgacg aggcgtttcc
     3841 aaagattcaa caaattcttt caccgtatag taaggaacca gtttcttgcc tggattttcg
     3901 gcaatcactt cttccgtttt ctcacgggaa cggttgaaaa tagagacggt ataaccacgg
     3961 ctttcgatgt tgagcgcaag gttgcgcccc atcactgcca taccgacgac gccgatctgt
     4021 tgctttgaca ttgtttactc ctgtcagggg gtgttcaccg cgatgtatgc gcgacttgaa
     4081 tatgattgag atgttatctc aagttgcacg aatgtgtgta gtgctgaatt tcattaatgc
     4141 aacaaatacc gttcttcaat tctgtatacc ggcatctacg tgtaatcaaa cctacgcaca
     4201 catataagcg tagataaacc tataacatca agaaatcaaa gaggtaatat ttcccagcac
     4261 attcgtaaca tattgcttag cctgattttc atcactggcc tcagcataac aacggagttc
     4321 tggagcatta ccggaaggac ggagatgtat aatatcacca ttatttaaag taaatcttgc
     4381 accatccgtt tcatcaatag cggaacagga taatgattca taacctaaac tattaaagaa
     4441 attattgggt gacgatatgg cattttttat aatttgttga cttgaatctg aagggaagtt
     4501 tttaactcta tctgaccaag tgaatcgctg aggaagatta ttaatcagtt gagaaatagt
     4561 actattgcga gaagctatta agagcattaa tgctggtaac acagcatctc gtgtaggtaa
     4621 cgattttaat tccttgccat taatttgtaa atcagaggca aggagaaaac caccattagc
     4681 ttcaaaacca gcgactgaat caaattgctt agcaagatct gcaaatgctg caattacata
     4741 aggagaacct attttcgttc gttgtatact tgcaaattta ttagactctt caattgctgt
     4801 attacaactc actggaatag ccaacgcctt gatattaagt tcaatagcag taagtagtcc
     4861 cagaatatcg cctcttaacc attcaccatt ttcatcggca actaaaggac gatcaccatc
     4921 gccatctgtt gagaaaatag catcaagatt atatttttta gaccactctc ttgcaagtat
     4981 acgatcttca tcgcttactg cttccgtatc aatcggaacg aactcatcac tcctaccgag
     5041 ggaaatgacc tctgcaccca tttgattaaa aagaggagca tataaatcgc gtcccgcact
     5101 agaatgttca tataccccta ttcttttccc ctttaaaatc tcgggattaa aaagagaaac
     5161 ataacgctcg aggtagtagt ctgcaccttg agtgcttgtt tcaagatgag gtaatacacc
     5221 tacaggcgaa aatgtatact cactatttat aattgcgagc tcatcctctt tagtgatttc
     5281 accatcaggt ctgtagaatt ttaagccatt acgatcgaaa gggatatgac tccctgtgac
     5341 cattatgcag ggaatgtttt tctgcatcga ataatgagct aatgcaggag tcggaattac
     5401 accatgatat tcaacctcga atccatgttg ttttatagca tacgcgcatg cctgagcaat
     5461 ttcgtaacta cttggacggt tatcaatggc taaggcaact ttattaaaat tgtatttgtc
     5521 atcaataaca gaaagaaacg catgagtgaa cgcagcacaa acatcatggg aaaaatcgat
     5581 cactaaaccg cgagcaccac t
//
LOCUS       DAZFAH010000254         5596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFAH010000254 DAZFAH010000000
VERSION     DAZFAH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.256"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..335)
                     /locus_tag="ACHJWB_004417"
     CDS             complement(24..335)
                     /locus_tag="ACHJWB_004417"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4315922.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            534..1232
                     /locus_tag="ACHJWB_004418"
     CDS             534..1232
                     /locus_tag="ACHJWB_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4315923.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1277..2176)
                     /gene="eamA"
                     /locus_tag="ACHJWB_004419"
     CDS             complement(1277..2176)
                     /gene="eamA"
                     /locus_tag="ACHJWB_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4315924.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2371..3558
                     /gene="ydeE"
                     /locus_tag="ACHJWB_004420"
     CDS             2371..3558
                     /gene="ydeE"
                     /locus_tag="ACHJWB_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4315925.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSHQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3685..3780
                     /gene="mgtS"
                     /locus_tag="ACHJWB_004421"
     CDS             3685..3780
                     /gene="mgtS"
                     /locus_tag="ACHJWB_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4315926.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3780..3884
                     /gene="mgtT"
                     /locus_tag="ACHJWB_004422"
     CDS             3780..3884
                     /gene="mgtT"
                     /locus_tag="ACHJWB_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4315927.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3999..4889)
                     /gene="dgcZ"
                     /locus_tag="ACHJWB_004423"
     CDS             complement(3999..4889)
                     /gene="dgcZ"
                     /locus_tag="ACHJWB_004423"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4315928.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5144..5536)
                     /gene="ydeI"
                     /locus_tag="ACHJWB_004424"
     CDS             complement(5144..5536)
                     /gene="ydeI"
                     /locus_tag="ACHJWB_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4315929.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1528 a         1342 c         1168 g         1558 t
ORIGIN      
        1 aatagtttaa taatattaga ttcttactct tgcagtagtt tcatcgcaaa ttcaagaact
       61 gcctgtccgt tcatggtgcc gtagtctttc atctcgataa cggctacagg cattttcccc
      121 tgtgcaacgg cttcaatctc tggtttctga aagcgaactt gcgggccaag taaaacaacg
      181 tcaacttcgt tatttttaat tttcccttta gcctctgcaa tcgccaatgc ggaaatatta
      241 acttcaagtg aaatagcgtt agcatgatca atcatacgtt taaccagcat actggttgac
      301 atgcccgcag cgcaaactaa caatattttt ttcatatttt cccttactgg tatataacag
      361 attacattgt ttaaatatca tatcccgcag tagctatacg tcctggtaca ttgatcacag
      421 taatacagtg ataaagttgt tcaacatcag ggtgattatt aaacgactcg gccatacgat
      481 aaaccaatca ggactcaact ccttgttttc aatggctctt aaaaggtgca aaagtgattt
      541 ttcaaaagat tgcccgctta cttaaatccg aaatcaatgg caattcatgg catgttggtg
      601 atttgttgcc gtcagaagcg gaactcgctg ttcgttataa tgtttcgcgt aataccctgc
      661 gtaaggcatt atccttgctg gaaggcgaag gaattattca cagaaagcat ggttcaggaa
      721 catacattca aaaaaagaat tttgttgcac acattgatca catgaacagt ttcagtgaaa
      781 ttgcgcataa aagcggcaaa gaggcaggga gtcagattat gaaatttgaa gtgcaggatg
      841 cttcacccac tattgcgact gagctgaatt tagtgactgg tgagcaggtt tattacataa
      901 agagactgcg attcattgag gataatgcag cacaattaga agaaacgtgg atgtcagtag
      961 cacgttttcc tgatttaacc gtgtcacata tgcaaaaatc caaattttca tatattgaga
     1021 atgaatgcgg gatcaaaatc attggcacct ttgaaacttt ctcccctact tttcctaccc
     1081 cagaaatcgc cagtatttta cggatcagcc cacgggatcc catacttaaa attcagaccc
     1141 aggctgtgga cagtaactct attccgctgg attattcgtt actttacagc aatattttcg
     1201 agttccaggt aaagtacttt ttcccgcgat aaaagcagac gcacagagtg ctgacatcac
     1261 attctgtgcg tatggtttag cctctaacct ttaccgcttt acgccaccgc aagccaaata
     1321 cattgatata cagcccggtc ataatgagca ccgcacctaa aaattgcaga cccgttaagc
     1381 gttcatccaa caatagtgcc gcacttgcca gtccgactac gggcaccagt aacgataacg
     1441 gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca taaccaacaa
     1501 ttgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatcg atagtaacca
     1561 gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     1621 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     1681 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca
     1741 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     1801 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa
     1861 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     1921 caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     1981 atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg
     2041 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     2101 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     2161 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     2221 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     2281 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaaacaaa
     2341 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ttctaccagc
     2401 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg
     2461 accatttact tgagtcacca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     2521 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     2581 gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt
     2641 cctttagtga ataacgtaac gctggtggtg ctcttttttg ccctcattaa ctgcgcctat
     2701 tccgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     2761 acgaaaatct tctcaatcaa ctacactatg ctcaacattg gctggaccat cggtccgccg
     2821 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tctggctggc agctatctgt
     2881 tccgcgtttc ccatgctttt cattcaaatt tgggtaaagc gcagcgagaa aatcatcgcc
     2941 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     3001 tttacctgct ctggctttct ggcttctttt gttagcggtg cctttgcctc gtgcatttca
     3061 caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt
     3121 ccggttaatg ctgccatggt agttacgttg caatattccg tgggccgccg ccttaacccg
     3181 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg tctggtcggt
     3241 tttatttttt ccggcaacag cctgctattg tggggtatgt cagctgcggt atttactgtc
     3301 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     3361 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca
     3421 ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg
     3481 ttggtgatca ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag accgtggggg
     3541 cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag ccttcgttta
     3601 ggttacctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg aggaaacaca
     3661 ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat ggccgtactg
     3721 ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg ggatgactaa
     3781 tgaacggaga taatccctca cctaaccgcc cccttgttac agttgtgtac aaggggcctg
     3841 atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt gcatggatag
     3901 atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg atcgttgaca
     3961 cagtagcatc agttttctca atgaatgtta aacggagctt aaactcggtt aatcacattt
     4021 tgttcgtcaa taaacatgca gcgatttctt ccggtttgct taccctcata cattgcccgg
     4081 tccgctcttc caatgaccac atccagaggc tcttcaggaa atgcgcgact cacacctgcc
     4141 gtcacggtaa tgttgatatg cccttcagaa tgtgtgatgg catggttatc gactaactgg
     4201 caaattctga cacctgcacg acatgcttct tcatcattag ccgctttgac aataatgata
     4261 aattcttcgc ccccgtagcg ataaacagtt tcgtaatcac gcgtccaact ggctaagtaa
     4321 gttgccaggg tgcgtaatac tacatcgccg attaaatgcc cgtaggtatc attaaccaat
     4381 ttaaatcggt caatatccaa caacattaaa taaagattca gaggctcagt gttgcgtaac
     4441 tgatgatcaa aggattcatc aagaacccga cgccccggca atcccgtcaa aacatccata
     4501 ttgctacgga tcgtcagcaa ataaattttg taatcggtta atgccgcagt aaaagaaagc
     4561 aacccctcct gaaaggcgtc gaaatgcgcg tcctgccagt gattttcaac aatagccagc
     4621 attaattccc gaccacagtt atgcatatgt tgatgggcag aatccattag ccgaacgtaa
     4681 ggtaattcat cgttatcgag tggccccaga tgatcaatcc accgaccaaa ctggcacagt
     4741 ccataagaat ggttatccgt tatttctggc ttactggcat ctctcgcgac cacgctgtga
     4801 aacatactca ccagccactg gtagtgggca tcgatagcct tattgagatt taacaagatg
     4861 gcatcaattt ccgttgtctt cttgatcatt gccactcctt tttcacagtt ccttgtgcgc
     4921 gctattctaa cgagagaaaa gcaaaattac gtcaatattt tcatagaaat ccgaagttat
     4981 gagtcatctc tgagataaca ttgtgattta aaacaaaatc agcggataaa aaagtgttta
     5041 attctgtaaa tcacctctgc attatcgtaa ataaaaggat gacaaatagc ataacccaat
     5101 accctaatgg cccagtagtt caggccatca ggctaattta tttttatttc tgcaaatgag
     5161 tgacccgaac gacggccggc gcgcttttct tatccagact gccgctaatg ttgatcatct
     5221 ggtccggctg aacttctcgt ccatcaaaga cggccgcagg aataacgaca ttaatttcac
     5281 cgctcttatc gcgaaaaacg taacggtcct ctcctttgtg agaaatcaaa ttaccgcgta
     5341 gtgaaaccga agcgccatcg tgcatgtttt ttgcgaaatc aacggtcatt ttttttgcat
     5401 catcggttcc gcgatagcca tcttctattg catgaggcgg cggtggcgct gcatcctgtt
     5461 ttaaaccgcc ctggtcatct gccaacgcat aaggcatgac aagaaaactt gctaatacaa
     5521 tggcctgaaa tttcatacta actccttaat tgcgtttggt ttgacttatt aagtctggtt
     5581 gctattttta taattg
//
LOCUS       DAZFAH010000255         5557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFAH010000255 DAZFAH010000000
VERSION     DAZFAH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.257"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="ACHJWB_004425"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="ACHJWB_004425"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4315930.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1532..2302)
                     /locus_tag="ACHJWB_004426"
     CDS             complement(1532..2302)
                     /locus_tag="ACHJWB_004426"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4315931.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVD
                     AIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2380..3180)
                     /locus_tag="ACHJWB_004427"
     CDS             complement(2380..3180)
                     /locus_tag="ACHJWB_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4315932.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3421..4335
                     /gene="yafC"
                     /locus_tag="ACHJWB_004428"
     CDS             3421..4335
                     /gene="yafC"
                     /locus_tag="ACHJWB_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4315933.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHJWB_004429"
     CDS             complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHJWB_004429"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4315934.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5298..5374)
                     /locus_tag="ACHJWB_004430"
     tRNA            complement(5298..5374)
                     /locus_tag="ACHJWB_004430"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5338..5340),aa:Asp,seq:gtc)
BASE COUNT         1447 a         1349 c         1379 g         1382 t
ORIGIN      
        1 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
      661 gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
      721 aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcgcg tgtgcacctg agtagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
     1021 caagtgggcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagcatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag
     1261 gccaaagttt gacgattggt gcaggcagta gcgcacagcg gttggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgaca
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
     1501 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga
     1561 tgatatcact ggtgaaagag ccatcagtct gcaaggaaaa atacgttctc acctctgtcg
     1621 atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta cgcattctca
     1681 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat
     1741 tggcttcatt gattaacgtc aaccactcac cgctggcgta gtttcgtacg tgagaggtat
     1801 cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tgggtggccc ggagacatta
     1861 cgtccatcac aatcagccta ccgccaggtt tcaatatccg gttcacttca cgcagtgctg
     1921 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttat
     1981 cggcaaatgg cagactttcg gcataaccct ggcgggtggt gatatttttc agttgccgga
     2041 cttcggcagc ttgtgccacg acatccagca tgtgggcaga taagtcatac gccaccaccg
     2101 tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta
     2161 cacttgcacc aggataatca gccagacgca ccgccaggcg ctgtaaatct cgtccggatg
     2221 catgtacggt actggtcaga tattcgcagg cttgagagct aaactgcttt tctacgtggt
     2281 catggtggga ttgtgttgtc attgcgctgt ccttttgttg tttgagtata aaaggacagc
     2341 gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg
     2401 actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccaata cagaagcttc
     2461 gctgacgttc agaccacggt agaaaacaaa atcgagcggg cggccaaacg cccgacggcg
     2521 ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc
     2581 gttcatcctt ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccgctgtg
     2641 gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca cgcccagact
     2701 gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac
     2761 cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca
     2821 atacactgga tgcgccgccg aaagggtcat tacaccggaa ggatgttgtg gcagcacgaa
     2881 agcgggcacc tgatcggcgg caagatagtt agcggtcgca aactgtacta actctggcgt
     2941 tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga
     3001 caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc
     3061 ggtacttaac ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg
     3121 taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac
     3181 ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta
     3241 tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt
     3301 acacaggtca gaattttgct cattattgag aacattaaat cggatgacat atcacgcatc
     3361 ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa
     3421 atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtagaaag cggcagcttt
     3481 agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc agtgaaaaag
     3541 ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact tagcctgacg
     3601 gaagaaggcc agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca
     3661 gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttgcgcat cgatgccgca
     3721 actccagtgg tgctgcactt tctgatgccg ttgattaagc ctttccgtga acgctatccg
     3781 gaagtcactt tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg
     3841 gatgtcgcga tacgcgctgg tacgttaacg gattccagct tacgtgccag gccgttattt
     3901 aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa
     3961 acgatcgacg atttaaagca acatatttgc ctgggattca ctgaacccgc ttccctcaat
     4021 acctggccga tagcccgtag cgatggacaa ttacatgagg tgaagtacgg tttgtcatcc
     4081 aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc
     4141 gactacatga tcgacaaaga aatcgctcgc ggagaattgg tggagttaat ggcagataaa
     4201 gtgttgccag tggaaatgcc attcagtgcc gtctattaca gcgaccgtgc ggtaagtacg
     4261 cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct
     4321 gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg
     4381 caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta
     4441 agattacttt ccaggttttt acgtttagta gaagaaggaa ttactgagta accttccccc
     4501 atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca
     4561 ataacctcat ctttcagggc cttaccatac gccagcgtca tataggaagt aatatggatg
     4621 ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga gagttcaatc
     4681 tggttagtag cgatgttttc agcaccaaca gcagcaatcg ctttttccat caacgggatc
     4741 gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc
     4801 tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga
     4861 tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat caatttgtct
     4921 ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca
     4981 ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt
     5041 gcgcgataac caagttcaag cgccgttatc acagatgaaa taacaacgtc gtctttcaga
     5101 cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt
     5161 cgttaactga ggtgattatg ggcttcagac tgttgaggaa aaagagcgta aaatgcagag
     5221 gatttttgcg attctggcaa taatagatat acagacgaaa aaaaacctcg agcgttaact
     5281 cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac
     5341 aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt
     5401 ttatgaccgg cttactgctt aatttgatgc ctggcagttt atgaatcact tcgtgattca
     5461 cccttcgggc cgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5521 gagtagtgaa ctgcctcaaa ggtcagtgcc attcttt
//
LOCUS       DAZFAH010000256         5527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFAH010000256 DAZFAH010000000
VERSION     DAZFAH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.258"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..2133)
                     /gene="glyS"
                     /locus_tag="ACHJWB_004431"
     CDS             complement(64..2133)
                     /gene="glyS"
                     /locus_tag="ACHJWB_004431"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4315935.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="ACHJWB_004432"
     CDS             complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="ACHJWB_004432"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4315936.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3149..3448)
                     /gene="ysaB"
                     /locus_tag="ACHJWB_004433"
     CDS             complement(3149..3448)
                     /gene="ysaB"
                     /locus_tag="ACHJWB_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4315937.1"
                     /translation="MMMNAFFPVMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3623..4618
                     /gene="wecH"
                     /locus_tag="ACHJWB_004434"
     CDS             3623..4618
                     /gene="wecH"
                     /locus_tag="ACHJWB_004434"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4315938.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALNNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4660..5097)
                     /gene="yiaA"
                     /locus_tag="ACHJWB_004435"
     CDS             complement(4660..5097)
                     /gene="yiaA"
                     /locus_tag="ACHJWB_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4315939.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5143..5484)
                     /gene="yiaB"
                     /locus_tag="ACHJWB_004436"
     CDS             complement(5143..5484)
                     /gene="yiaB"
                     /locus_tag="ACHJWB_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4315940.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1343 a         1429 c         1354 g         1401 t
ORIGIN      
        1 ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg
       61 ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag
      121 catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa
      181 gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc
      241 ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat
      301 ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt
      361 cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg catccagggt
      421 acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag tcggacgacg
      481 cgccagtacc gcctggatgg tgtcaacggt ataaccttcg tcctgatacc aggcgcggaa
      541 gcgaccgagc ataaagtcga taacatcatc aactacgttg gcattagtca gcttatcgcc
      601 atacagacgc accgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac
      661 gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt
      721 cggatgctgg ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag
      781 cgcacaggct accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc
      841 attcagcgcc accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat
      901 aacgccctgg gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag
      961 caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc
     1021 cagcgcctgg atgcggtcag ttttgtcgcg cagcgtaccc agctgttgct ggaacagcac
     1081 ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat tgaagaagaa
     1141 ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa tttgctgcgg
     1201 atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc catcgttcgc
     1261 atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt cagccggcac
     1321 cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc
     1381 cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc
     1441 ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg
     1501 ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat
     1561 aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac
     1621 tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc
     1681 gccccaacgc atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg
     1741 cagcagtgct tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt
     1801 atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc
     1861 tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt
     1921 ttcgatttca cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg
     1981 cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt
     2041 aaaattcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt
     2101 gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca
     2161 catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag
     2221 ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc
     2281 cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg
     2341 attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag
     2401 gaagtccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa
     2461 cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct
     2521 gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac
     2581 cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc
     2641 gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc
     2701 ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag
     2761 gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg
     2821 ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac
     2881 ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt
     2941 tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta
     3001 atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt
     3061 tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata
     3121 tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat
     3181 ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc
     3241 ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt
     3301 ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta
     3361 cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca
     3421 ttaccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta
     3481 ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt
     3541 agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt
     3601 ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg
     3661 gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca
     3721 tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag
     3781 cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc
     3841 gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta
     3901 cattgcgctg tttacctcca tcaatatgga gttagcgctg aacaacctgc tgcaaaagcc
     3961 agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct
     4021 gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg tgattggcat
     4081 tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa
     4141 cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat
     4201 agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac
     4261 gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc
     4321 cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac
     4381 tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca
     4441 ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat
     4501 tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc
     4561 gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt
     4621 aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg
     4681 ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc
     4741 tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa
     4801 taatgtcgca ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag
     4861 gcaggtcata taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt
     4921 ttgataagac gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc
     4981 atttaactgc atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag
     5041 agctatccat gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac
     5101 agtattcctt tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa
     5161 acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc
     5221 attgataggt gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa
     5281 ctggcagata tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc
     5341 atagttacca aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa
     5401 tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt
     5461 tgccacagtt tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt
     5521 aaccaac
//
LOCUS       DAZFAH010000257         5522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFAH010000257 DAZFAH010000000
VERSION     DAZFAH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.259"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..701
                     /locus_tag="ACHJWB_004437"
     CDS             3..701
                     /locus_tag="ACHJWB_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4315941.1"
                     /translation="MRDKAKRDLSHISIEIPKSVTQVIKEKIRDMIIHGDLSLGQPIS
                     ENELSNILAVSKTPIREAFILLSHNENLINIIPRSGTFVFSVTDKDINDLIKMRVILE
                     QGAIREAMEKNANNVTIELSSILSKSAKINAERDIQAYLKLDHDFHYVFVKYADNKYI
                     SQAHQLISARLLAIRYRLDFTTEYITNSNRGHATILDMLKNNNVEGVCNFITHHVSSG
                     FTERARKILSLSTQ"
     gene            779..1501
                     /gene="ubiG"
                     /locus_tag="ACHJWB_004438"
     CDS             779..1501
                     /gene="ubiG"
                     /locus_tag="ACHJWB_004438"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4315942.1"
                     /translation="MNTENLLKNNNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(1642..5358)
                     /gene="yfaL"
                     /locus_tag="ACHJWB_004439"
     CDS             complement(1642..5358)
                     /gene="yfaL"
                     /locus_tag="ACHJWB_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4315943.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGNG
                     QTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQ
                     GESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEQDDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         1402 a         1452 c         1255 g         1413 t
ORIGIN      
        1 gaatgagaga taaagcgaaa cgtgacttaa gtcacatatc aattgagata ccaaaatccg
       61 taacacaggt catcaaagaa aaaatcaggg atatgataat tcatggcgat ctcagtcttg
      121 ggcaaccgat aagtgaaaat gaactatcga atattctggc ggtaagtaaa acacctattc
      181 gtgaagcttt tattttactt agtcacaatg aaaatctgat caatatcatt ccacgcagcg
      241 gaacattcgt tttttccgta acagataaag atattaatga cctcataaaa atgcgagtaa
      301 ttctggagca gggagcaatt cgtgaagcaa tggaaaagaa tgcaaacaat gtcaccattg
      361 aattaagtag catactatca aaatcagcaa aaattaatgc cgaacgtgat attcaggctt
      421 atcttaaatt ggatcatgac tttcattatg tgtttgttaa atacgctgac aataaataca
      481 tctctcaggc acaccaattg atatccgcac gtttacttgc tattcgttat cgactggatt
      541 tcactacgga atatatcacc aattcaaatc gtggccatgc tactatttta gatatgctaa
      601 aaaataataa cgttgaaggt gtttgtaatt ttattactca tcacgtaagt agtggattta
      661 ccgagcgcgc acgaaaaatt ctctcgttat ctacacagta aaatgtgaat gagtttataa
      721 cactggcact tttcacctgc tatactgccc gacaaccttt atagactctt atgtaacaat
      781 gaacactgaa aatctcctta aaaacaataa cgtagatcac gaagagatcg ctaaatttga
      841 agccgttgcc tcccgctggt gggatcttga aggtgagttc aaaccgctgc accgcattaa
      901 cccgctgcgt ctgggctata ttgccgaacg tgctggcggt ttatttggca aaaaggtgct
      961 cgatgtcggt tgtggcggcg gcattctggc cgaaagtatg gcgcgcgaag gcgcgacggt
     1021 gaccggtctg gatatgggct ttgagccatt gcaggtggca aaactgcacg cgctggaaag
     1081 cggcattcag gtggattatg tgcaggaaac cgtggaagag cacgcggcaa aacatgccgg
     1141 gcagtatgat gtggtgacct gcatggagat gctggagcac gtccccgatc cgcagtcagt
     1201 ggtcagagcc tgtgcgcaac tggtgaaacc aggcggcgat gtctttttct cgacgcttaa
     1261 ccgcaacggc aagtcatggc tgatggcggt ggttggcgcg gaatatattt tgcgcatggt
     1321 gcccaaaggc acgcatgatg tgaagaagtt tattaaaccg gcagaattgc tgggctgggt
     1381 ggatcagacc agtttaaaag agcggcatat cactgggctg cattacaacc cgatcactaa
     1441 tacttttaaa ctcggccccg gcgtggatgt gaactatatg ctgcacacgc agaataagtg
     1501 aggttgttgt ttggattcat ccggcaaccg cgccaatgcc tgatgcgacg cttgccgcgt
     1561 cttatcaggc ctacaaatgc tccccgtagg ccggataagg cgtttacgcc gcatccggca
     1621 accgtgccga ctagacagtg attaccattt caccgtcatc gacaaaaacc ctgccatctg
     1681 ggcaaaatca tcactccctt tctgccacgc cacgctgccg cgcagggaca ctcgctgact
     1741 gatattgccc gtgactccca cttttatttc accccgtcgc ttcaccgcat cgtcactgat
     1801 agtgctgcca tcttcttcaa tttccgtcgc atggggatcg tgataataat tcagatctaa
     1861 tgttggtgtg acaccaaccg cggtacgcca ttcgctgtgt aaacccagcc gcgtctgaat
     1921 atcatccccc tgcgattgtg acacgcgcgc atggttagcg gcggtaaaat catcctgctg
     1981 cacgccctga taaatcacct gcgcctgcgg ttcaatcacc acaccacgcc ccggtaacca
     2041 ctgatacccg gcttccagcg aggcgataat ccccgacgag tggtaatgat ctgtgccatc
     2101 gtcttgttcg gaaacatcat tgttaaacca cgcgtattgc agccagctat ccagccaggc
     2161 cccctgcttc tgattaccgt gctgatacca gcttgaggtc agcccaacgg cataaccgtg
     2221 gttctggtta tcggcgcgag ttccggtcat attcgagcgg ctctcgccct gattatcact
     2281 gtagccacca accgccccaa gcatccactc gccatcatcg ccccagcgcc cgctaaacag
     2341 gtcaccgcta agctgcaccg tagaagtgtc ttcatgctga gccagttgcc ctgccgccgt
     2401 gtaatgataa cgtccgccga taacacgtaa attcagcgtc tgaccattgc cacctgcatg
     2461 atcgcgtcgc tccatcataa acgcctgatt tgccgcccgc agattattga gataaccgcc
     2521 aactttggca ttcagcaccg gctggtaagc ggggattgga tcgggtgttg ggtcagggtc
     2581 tggatcaggt ggcaatggtg gcgttacttc ttgcgatcgc agataccagt cgttgttatc
     2641 ttccaccagc gtgtagtcat atgctcccat attgacgtag ccgctgcctg ccagactgaa
     2701 cttcgcattg ttttgaaact gcgtgggatc ggctgcgaaa tcaaccactt taatgcctgt
     2761 cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt tgccagcagt
     2821 attaccgttc aacaccaatt gatcgcttac cgagtcatcg ccgtttaatt cgctatcgag
     2881 cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc cagcgacgcc
     2941 attttgcagg ctaaccgcac cggacgtatt ggtcagattg ccattaaccg tcacgtcgga
     3001 aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca acgtcacgcc
     3061 atcggtggca ttcaccagcg tcccatcgcc gttaaacagt gaggcattaa gggcgacaga
     3121 agtatcctgc ccggtcaggc gcaaaaccgt accatcgctg atgtcgatag tgccgctgga
     3181 agtagcatca atagattgaa tatcctgatc cgcgccagta acgaacgtcg ccccatcacc
     3241 gacccacagt gacgaagcat aaggaaggat atccgcgaca tcgcctttca gggtgccttc
     3301 ttcgacacgt accgccgact gcgttgtacc gctggcggtc agctccagcg tacccgcccc
     3361 cgttttagtc aatgtgctac cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc
     3421 gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggctc gcatttcaat
     3481 atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa tctccatcac
     3541 gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat ctccaagctg
     3601 tgagttgtcg ctcacctcca gcataccgga ggcgatttgt gttgtgccca aataactatt
     3661 attgtttgcc agacttacct gcccgtcgcc cgcatcgacc accacgtaga cgccatcttt
     3721 cccgtcaccg ccgatatgca tatcgtttcc acctgcaaga tctaccacat cctgactgcc
     3781 gataaccgtt ccgctgctgc cgctgacttc aaggccatcg ttgtatgatg tgccactctg
     3841 ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg actgcggatc
     3901 gtcctggaga gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg caccatcatc
     3961 aacgactata tcgccggtta gcgccatcga ctgcgcccct gccagcacgt agctgccgtt
     4021 ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc cctgattaac
     4081 agtaacgttg ccaccagcat cgatatttaa agtgccattc tgaaagcccg tcaatgcgtg
     4141 cacaaaagtt tgttgggtcg agccaacgtt tagctcagcc tgattctgat actgatcaat
     4201 actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg tatcgccgac
     4261 attcatcagg gagttgctgc ggcccagggt aacttcaccg ttttcaatct gcatctcgcc
     4321 agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt tggtgattaa
     4381 cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccga taaccagcgt
     4441 ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa agccacccgc
     4501 cgcagtggaa ggaccatctc catagcctgc tgcgctgttg ttctcatcca ctaacacgcc
     4561 gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag ccgtcgcgct
     4621 attggtatat atcgccccgc cataaccttc ggcagtgtta tttgtaaagg cggtgttatt
     4681 tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa catcgattgc
     4741 cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat caaaaataac
     4801 atcacttaaa taaacgtcat tattaatggt ataaattgcg ccacctttgc catcattagc
     4861 gatgttattg cgaaacatgg cgttagtgac acgtaaatcg acggcaccag tatcattagt
     4921 accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccagaaa aaataacatc
     4981 agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat tatattctcc
     5041 tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta atagtgaaaa
     5101 ttccgctcct tgttgcaaaa atacggctcc tccgctggcg ttattggtca tatccgaaaa
     5161 aaccagccat tgcccatcgg cgacactcca gtcctgcgta atgcctgaaa ggctttgcct
     5221 gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg ccgccgcgac
     5281 accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat actccttgcg
     5341 tagaaagata atccgcatat taatctgacc atcattttat ataagacggc atatttaaac
     5401 gcattataca atatgggtta tctttctttt gtaaattgtc caacccgatg caatggctat
     5461 tttgttatta tttgatgaat aatatcagtg cgtcataatt caagttaata accttaaggg
     5521 at
//
LOCUS       DAZFAH010000258         5482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFAH010000258 DAZFAH010000000
VERSION     DAZFAH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.260"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..168
                     /locus_tag="ACHJWB_004440"
     CDS             <1..168
                     /locus_tag="ACHJWB_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315944.1"
                     /translation="SSEEFLSDNTLEKDENSPDNCFEVVKYNTYEAYNSENLYFTREE
                     YTYDYDLLNAI"
     gene            complement(669..1045)
                     /gene="rnpB"
                     /locus_tag="ACHJWB_004441"
     ncRNA           complement(669..1045)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJWB_004441"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1078..2223)
                     /gene="garK"
                     /locus_tag="ACHJWB_004442"
     CDS             complement(1078..2223)
                     /gene="garK"
                     /locus_tag="ACHJWB_004442"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4315945.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2320..3210)
                     /gene="garR"
                     /locus_tag="ACHJWB_004443"
     CDS             complement(2320..3210)
                     /gene="garR"
                     /locus_tag="ACHJWB_004443"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4315946.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3240..4010)
                     /gene="garL"
                     /locus_tag="ACHJWB_004444"
     CDS             complement(3240..4010)
                     /gene="garL"
                     /locus_tag="ACHJWB_004444"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4315947.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4026..5360)
                     /gene="garP"
                     /locus_tag="ACHJWB_004445"
     CDS             complement(4026..5360)
                     /gene="garP"
                     /locus_tag="ACHJWB_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4315948.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHIFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1349 a         1538 c         1295 g         1300 t
ORIGIN      
        1 agctcagagg aattcctttc agataataca ctcgagaaag acgaaaattc tcctgacaat
       61 tgctttgagg ttgtaaaata taacacatat gaagcatata actcagagaa tctatatttt
      121 accagagagg aatatacgta tgattacgac cttttaaatg cgatatagac ttaataactt
      181 cttacaaaat ctataattaa taccgtagat attcattcat cttaccctgt gcaccaaaag
      241 caaaaactca ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta
      301 cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt tgtaggcagg
      361 ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc
      421 ggaacggttt attacgtaaa tcaggtgaaa ctgaccgata agccgctttc ttttgggtat
      481 agcgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg
      541 ttttttacgt aaaccaggtg aaactgaccg ataagccgct ttcttttggg tatagcgtcg
      601 tggacagtca ttcatctttc cgctcctcca aaagcaaaaa cccgccgaag cgggttttta
      661 cgtaaatcag gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag
      721 gccagcaatc gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat
      781 gaacccctat ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg
      841 cgcggtgcgc tcttaccgca ccctttcacc cttacctgat cccgcttgcg cgggccatcg
      901 gcggtttgct ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct
      961 gccctatgga gcccggactt tcctcccctc cgcccgtctc ccccgaagag gacgacgacg
     1021 aagcggcgac tgtctggtca gcttcggcgc gcagtataga gggtttgcgc gcccttgtca
     1081 ccccgcgttg cgcattccaa tcgccagtgt cgcggcgata ttacgtgaag cacggcagat
     1141 attgtcataa gccccgcgga atgcttcgtc caacgtacct atgctggtca atacgctgaa
     1201 gaccgcatcg atgccatgct gatgtacaac gccaacatca tcggtcaggc tacccgcaat
     1261 gccaatcacc ggtttatggt acttcttcgc cacgtttgca acgccaatcg gtaccttgcc
     1321 gtgaatactc tggctgtcaa tacgcccttc accggtgatc accagcgtac aatcgtgaat
     1381 atgttcctcc agattcagcg ccgtagtgac gatttcaata ccacttttca gttccgcacc
     1441 aagaaacgcc atcagcgccg cacccatacc gcccgcagct cctgcgccgg ggacatcttt
     1501 cacatcaaca tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattgtccag
     1561 ctcaacaatc atcgcttcac tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt
     1621 atcgcccacc agcggattgg tgacatcaca agcgacgcga atgacgcaat cttttaagcg
     1681 cggatcgagg ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc
     1741 aatttcattg ccgttggcgt cgcataattt cgcccccagc gcctgtacca tgcctgcgcc
     1801 gccatcattt gtagcgctac cgccaatgcc gataataatg tttgtcgcgc cgctctccag
     1861 cgcctgcagg attaactcgc ctgtgccgcg tgaagtggtc acgagtggat cgcgtttttc
     1921 cgcaggtacc agctccagcc cactggccgc cgccatttca ataaacgcgg ttttgccatc
     1981 gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg
     2041 acgttcagaa ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc caccgtcggc
     2101 aaccggaata gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc
     2161 ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt
     2221 catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc
     2281 caggcccgga ttgcaatacg ccatccgggc acgacgtcat taacgagtaa cttcgacttt
     2341 cgccagtttt tcgtagtagc acgccagggc gctatgatcc gccgttccta aaccatctgc
     2401 tcgcagtgcc tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc
     2461 gtgggaagta tccagcgcat ttgccagatc cttaatatgc agatcaatac ggaagcccgg
     2521 cttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag
     2581 tccaccgcga attgcctgat acaccaggtc cgggttaacg cccgctttgg ttgccagcgt
     2641 tagcgcttct gacatcgcgg caatattcag cgccacaatg acctgatttg ccagtttggt
     2701 gacgttacct gcaccgattt ccccggtatg caccacggaa cccgccatcg ctttcatcaa
     2761 atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc
     2821 gatggctttc ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt
     2881 cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac
     2941 cgtacctggc ttcgcgcctt caatgatgcc attctcaccc agcgccacct ctttcacatg
     3001 aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt
     3061 agacgctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc
     3121 aaccaccagc gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc
     3181 caggccaata aaaccaactt tcatagtcat atcaatcatc tctcttgttg cggtggtggt
     3241 tattttttaa aggtatcagc cagtttctga gtggcagagc ggaagacgcc aagatcgctg
     3301 ccgacagcca caaacgtcgc gccccattcc agataacgac gcgcatcggc ttcgaccggc
     3361 gcgagaatac cgctgggttt gccgtgcgca ctggcacggt taaaaatgtg ctgaattgct
     3421 ttttgtacat ccgggtgcga ggcattgccg agatggccta atgccgcagc cagatcgctg
     3481 gggccgacga agataccgtc tacaccttcg gtagcggcaa tggcatcgac gttatctacg
     3541 ccctgctgac tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa
     3601 tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc
     3661 ggtgggtaac gggttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga
     3721 atcaggaagt tatagaaacc gatatccaga agacgcttaa taattaccgg ctcgttggtc
     3781 ggcactcgca ctactggcgc gctggcgctg cctttcaggg ccattaactg cggaataaac
     3841 gtggaaatat cgtttggcgc atgttcgcca tccagcacca gccagtcaaa cccagccaaa
     3901 ccaagaactt cagtgctaat cgggttagag agtgctgacc agcaaccaat ttgtacctgt
     3961 ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc
     4021 tttgcttatt tctgcaattc catacgttta atgtcgccaa ctacgaagag gtagcagacc
     4081 atcgccatca gcgctgaaca tcccacgaaa accagtgctg cattgaagga gtgcagttca
     4141 cttaccaggt agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta
     4201 aagacgccgc cgcagaggcc aacaatctct ttcggcgcgg tgtcagaaat caccggccag
     4261 cccagagcac caaatccttt gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg
     4321 gtgttgttgg tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatc
     4381 ggtagcttac gtgccagggt cagggataaa ccgcgtttga tcagataatc cgagaagaca
     4441 cctcccagca cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac cagacccact
     4501 ttcagaatcg acatgccttt ttcctgcacc agataaatcg ggaaccaggt gaggaagaac
     4561 caggtgatgg tgttgataaa atattgtccg aaaaatacgc ccaacatcat gcggttagag
     4621 agcaattgct tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc cggctttttg
     4681 tggtccatat cgaccaccgc gccattttca gagataaact tcagctcttc cgcagacata
     4741 cgtgggtgat ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacca
     4801 atcaccccca taacggtgaa gatgtgctcc cagccccagg cgaaagtcag ccagccaagc
     4861 agcggcgaaa agagcgccag cgagaaatat tgcgccgagt taaagatggc ggaggcagta
     4921 ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatggc
     4981 gcttccgaga accccagcat aaagcgcata aagaacatgg agatccctgc ccaggccagc
     5041 gggaacatat caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag
     5101 gtgtaaactt ttttcgagcc aaacttatca agcagccagc cgccgggaat ttgcatcagc
     5161 aggtaggccc agccaaaagc ggagaagatg taacccatcg aaaccgcact taactgcaac
     5221 tcttttgcca cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg
     5281 gtaacaataa aaataatcag taatattaaa tagcgggtat gcacgccttt cttttttacg
     5341 tcaacggtgt ccagaatcat tttatttacc tcgggtactt atgctgattt ttattattat
     5401 ggtgaaggtg ttatttatga gttttattta tgccgttacg acaatgaact cgggaattag
     5461 tataagcagc gcgagaataa ta
//
LOCUS       DAZFAH010000259         5384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFAH010000259 DAZFAH010000000
VERSION     DAZFAH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.261"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..120
                     /locus_tag="ACHJWB_004446"
     tRNA            45..120
                     /locus_tag="ACHJWB_004446"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:ggt)
     gene            160..275
                     /gene="rrf"
                     /locus_tag="ACHJWB_004447"
     rRNA            160..275
                     /gene="rrf"
                     /locus_tag="ACHJWB_004447"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(506..1264)
                     /locus_tag="ACHJWB_004448"
     CDS             complement(506..1264)
                     /locus_tag="ACHJWB_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4315949.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1272..2375)
                     /locus_tag="ACHJWB_004449"
     CDS             complement(1272..2375)
                     /locus_tag="ACHJWB_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4315950.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2385..3566)
                     /locus_tag="ACHJWB_004450"
     CDS             complement(2385..3566)
                     /locus_tag="ACHJWB_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4315951.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3634..4659)
                     /locus_tag="ACHJWB_004451"
     CDS             complement(3634..4659)
                     /locus_tag="ACHJWB_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4315952.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5090..5311)
                     /locus_tag="ACHJWB_004452"
     CDS             complement(5090..5311)
                     /locus_tag="ACHJWB_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315953.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1477 a         1385 c         1289 g         1233 t
ORIGIN      
        1 tccccatgcg agagtaggga actgccaggc atcaaattta gcgtgctgat atggctcagt
       61 tggtagagcg cacccttggt aagggtgagg tccccagttc gactctgggt atcagcacca
      121 ctttttaggt taaagttcgg cagattagaa aagaatttgt ctggcggcag tagcgcggtg
      181 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
      241 gggtttcccc atgcgagagt agggaactgc cagacatcaa ataaaacaaa aggctcagtc
      301 ggaagactgg gccttttgtt ttatctgttg tttgtcggtg aacactctcc caaccaggac
      361 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
      421 acgcccgcca taaactgcca gacatcaaat caagcgaaag gccatccgaa aggatggcct
      481 ttttgctttt cgaactaaca ttcaattaat ggattacctg cgataaaaat gccctcgtac
      541 gctctgattt aggatgcgca aaaaattcat caggtgcagc ttgttccact atctccccac
      601 gatccataaa aattacccgg tcagcgacgg tacgtgcaaa ccccatctca tgtgttacac
      661 acaacattgt catacccgac tgcgccagcc caatcatcgt atccagcacc tctttcacca
      721 tctccggatc gagcgccgac gttggttcat caaacaacat aattttcggc ttcatacaca
      781 gcgaacgcgc aatggcaacg cgttgctgct gaccacctga aatctgtccg ggaaacttat
      841 gcgcatgttc ggcaattctc acccgctcaa ggtaatgcat cgccagagct tcagcctctt
      901 tcttaggcat cttgcgtacc caaatcggtg ccagggtaca gttctgtaga acggtcagat
      961 gagggaagag attgaaatgc tgaaagacca ttcccacttc ctgcctgacg cgctcaatat
     1021 tgcggatatc ttcattaagt tcgatgccat ctactacgat ccgtccctgt tgatgttctt
     1081 ccagatgatt aatacaacga atggttgttg atttaccgga accagaaggg ccacacagaa
     1141 cgatccgttc tcccggttgc acggttaaat ttatattttt caaaacatgg aattgtccat
     1201 accatttatt gacgttttcc agcgtaatca tcgcgttagc aggttgcagt aaaatttggc
     1261 tcatagtgtc ctcaatgcgg tgtacgcccg gtgttaaaac gtttttccag atgctggcta
     1321 tagcgcgaca tgctgaaaca gaagatccag tagatcagcg cggcgaaaac atacccttcc
     1381 gtcgacatac ccaaccaggc gggatcaacg gttgcctgct gaacgctact gaaaagatcg
     1441 aacaacccga taatgatcac caggctggta tctttgaaga gtgcgatgat ggtatttacc
     1501 agcccaggaa ttaccagctt caacgcctgt ggcagaataa ccagcccctg agttttccag
     1561 taacccaatg ccagtgactc tgccgcttca tattgccctt taggcagcgc ctgtaatccg
     1621 cctcgcacga cttccgcaac atatgctgac tgaaacagga tcacgccaac cagcgcgcgg
     1681 atcagtttgt cgatattggt gccttctgcc ataaacaaag gcagcatgac cgaagacata
     1741 aacaatacgg taatcagcgg tacgccgcgc cagaactcga taaaaatgac cgataagata
     1801 cgcacaatcg gcatatggga gcggcgacct aacgccagta agatccccca cggtagcgcc
     1861 cccgcaatcc caacggatgc aataattaaa gttagcgtca gcccgcccca ttgccgggtt
     1921 tcaacccgct ccagaccaaa aaaaccgcca tacatcagcc accagacaat cagtgggtaa
     1981 atcaccgccc agaccgcaat atagcgaccg cgatgcggga gtattttcca gaacattggt
     2041 gcgattgaca caaggccaat cagtaatgcc aggttaattc gccagcgttg gtcgtgtggg
     2101 taaagtccat acataaactg accaaatcgt tcgtggatga agacccaaca ggcaccggct
     2161 tttgtgcagt cggcacgcgt tgagccaacc caattagcct gcaaaaatgc ccagttcagc
     2221 aacggtggga tcagttccca catcaaccat atgcagccaa tagtcagcag gctattgctc
     2281 cagctggaga acagattttt tcgcacccac accatcgcgc ggcttgagtt atggctcgct
     2341 gggcgcgagg gctgagacag caatactttt gtcatcatga ctccttagcg ttcaacgatc
     2401 gcgatgcggc ggttatagat attcatcagc agcgagatag tcaggctgat aatcagatag
     2461 accgacatgg tcatggctat cgtctcgatg gcttgccccg tctggttcag cacggtgcca
     2521 gcaaacagcg aaaccatatc gggatagcca atagcggcgg caagagagga gtttttgacg
     2581 atgttgagat actggctggt taacggcgga ataatcactc gcagtgcctg gggaataatg
     2641 acctggcgta gcgtaaccgg gttgggtaat cccagtgacc gagccgcttc atgttgacca
     2701 taaggcactg cctggatccc agcgcggata atctcggcga taaatgcaga tgtataaacc
     2761 gaaagtgcca gcgttaaggc tgccagttca ggaattaaaa ccatcccgcc gcggaagtta
     2821 aagcctcgta gggctgggac atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt
     2881 aaaggcaaac caatgatcaa cactgcggcg atcggccagg tgcggcgcag ttgtccggtc
     2941 tttatctggt atgttttatt aaagcggaat agcccaacag aaaggactat agccataaca
     3001 acagccagga taaacgcaat aaatccgtct cccagctgcg gtgacggaat ataaagccca
     3061 cgattgctca aaaaggccag atcaaacgcg ctcacagcct ggcgtggtcc gggcaaattg
     3121 cgcaacacgg caaagtacca gaagaagatt tgcagcagcg ggggaatatt acggaagatc
     3181 tcaatataaa ttgtggatag ttttcgtagc agccagttat ccgaaagtct cgccagaccg
     3241 ataaagaagc ctaaaacaga agcgaacaca atacacaatg cagaaaccag tagcgtattg
     3301 agtaagccga caataaaaac gcgtccgtag gtgtcgccct gctggtaatc gatcaaatgc
     3361 tggacaatac cgaagccagc gccgcgatcc agaaaggcaa aacctgaagt aatgccacga
     3421 ttattgagat tcgttacagt gttgtgaaat aaccaaccaa caatgccgac aacagcaaca
     3481 acggcaagga tctggaataa ccaggcgcga accgtagggt tggcaaaaga gagtgagcct
     3541 ttaacggttg agcggcgatg agacatagaa acctcggaaa gcataacgct gaagagagcg
     3601 ccgccgtagc gacgcccgtt acctcacacc gacttaacgc acgggcggcg cgtactgaat
     3661 accgccgtta ttccagagat tattttgccc acgtttaatt ttcagcgggc tttctgaacc
     3721 tacgttacgc tcgaaaattt ccgagtagtt acccacctgt ttgatgatgt tataggccca
     3781 tttattatcc agcttcagat ccttgccgta atcgccttct ttacccagca gatgtgccat
     3841 atcaggcgtt gctggattag ccgctttttc atcgacgttc tgggaattga tgcccatctc
     3901 ttcagcattc agcatggcga aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc
     3961 acgacgaact accggcccaa gcggttcttt agagataacc tccggtaaga caatccattc
     4021 agctgggttg cttaatttga tgcgcagggc atacagttgc gactgatccg aggccagcgt
     4081 atcgcagcga ccagattcca gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg
     4141 tgtgtacttc atattgtttg ccttgaaata gtcggcaacg ttgagttcgg tatcagtacc
     4201 cgcctgaata cagacggtag cgccatccag ttctttcgcg ctttttagcc ccgctttatc
     4261 gtgcgtcagg aagccaatgc cgtcgtaata agtgacgccg gtaaatgcca ttcccatccc
     4321 ggcatcgcga gatgaagtcc aggtcgtatt acgggagagc aaatccactt cccctgactg
     4381 taaagcggtg aagcgttctt ttgctgtgag tggggtatat ttcactttcg tgtcgtcacc
     4441 aaatacagca gcggcaacac cacgacaaac atcaacatca atacctgaaa acttaccgtc
     4501 tgcatcggca taagagaacc caggtaatcc atcactgatc ccgcattgca caaaaccttt
     4561 tttctgaacg gcatcaagcg tcgcgccagc atgcgcctga tttgcaacgg caagcagcac
     4621 gctggcggca gccagtgtgg ctatcatcat ctttttcata atgcttcctg tgaggcgaaa
     4681 attatcgtta taggacgttt gggaaacgtc ttttacctgt ctgtggccat cctcttataa
     4741 gcaaaagtag tgccagcttt tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag
     4801 gaaataataa aaacagcgcc ccgcaatggt gcgaaaatga aacatgcgcc acattttggc
     4861 gcaaaatatt tacgcgtgtt ttactaagca gaataatgat tgttagcgtg agtatatttc
     4921 tggtgggaag tgtgaaagtt aaattgatgc gttatttcga aagcagcgaa ataacgcgat
     4981 ataacatgct aaatcagcag agaattagcc agcagaatat agcaatattt tatcttatgt
     5041 aaagatactg tatcgttaaa cagagcgcga aactccgcgc cctgttcagt tatttcgaca
     5101 gagcgatgat ccccatcgtc aggccagcaa cagccgctgc cccgccggca atagccccag
     5161 ccgtttccca gttatcctga gtcgtgcctt tcatacaggt attggtgtga accagacgac
     5221 cctgatcgtc ataaaccgga acgcagggcg aatcgtgagc acagcccgcc agcaatgcgg
     5281 tgagcaatgc cacaggaatt aatcttttca tggtgttacc tcagtaataa acgctaacgc
     5341 ttaataagtt aactctctta ttaacgagct gaatgccatt ttat
//
LOCUS       DAZFAH010000260         5341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFAH010000260 DAZFAH010000000
VERSION     DAZFAH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.262"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..234)
                     /gene="ypaB"
                     /locus_tag="ACHJWB_004453"
     CDS             complement(91..234)
                     /gene="ypaB"
                     /locus_tag="ACHJWB_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4315954.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            362..2647
                     /gene="nrdA"
                     /locus_tag="ACHJWB_004454"
     CDS             362..2647
                     /gene="nrdA"
                     /locus_tag="ACHJWB_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4315955.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2794..3924
                     /gene="nrdB"
                     /locus_tag="ACHJWB_004455"
     CDS             2794..3924
                     /gene="nrdB"
                     /locus_tag="ACHJWB_004455"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4315956.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3924..4178
                     /gene="yfaE"
                     /locus_tag="ACHJWB_004456"
     CDS             3924..4178
                     /gene="yfaE"
                     /locus_tag="ACHJWB_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4315957.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4232..4882)
                     /gene="inaA"
                     /locus_tag="ACHJWB_004457"
     CDS             complement(4232..4882)
                     /gene="inaA"
                     /locus_tag="ACHJWB_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4315958.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4962..5066)
                     /locus_tag="ACHJWB_004458"
                     /pseudo
     CDS             complement(4962..5066)
                     /locus_tag="ACHJWB_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5061..5303
                     /locus_tag="ACHJWB_004459"
                     /pseudo
     CDS             5061..5303
                     /locus_tag="ACHJWB_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1340 a         1410 c         1305 g         1286 t
ORIGIN      
        1 gtgatgactc gtgcttagat caatttttgc aatcattagc aaaaagatta ataaaccatc
       61 tatatcaatt tatccaacct attctgccgt tcaagaaatc gccgaacagt tatttttaac
      121 aaatttttct cttcccattg actttcccgg acaccttgtc tgacctaagg tgcgcgaaag
      181 ccactttttc cttcctgagt tatccacaaa gttatgcact tgcaagaggg ccattttcac
      241 actatcttgc agtgaatccc aaacataccc cctatatata gtgttctaag cagcttcccg
      301 tactacaggt agtctgcatg aaactattgc ggaaagaatt ccaaaaacag gtacgacata
      361 catgaatcag aatctgctgg tgacaaagcg cgacggtagc acagagcgca tcaatctcga
      421 caaaatccat cgcgttctgg attgggcggc agaaggactg cataacgttt cgatttccca
      481 ggtcgagctg cgctcccaca ttcagtttta tgacggtatc aagacctctg acatccacga
      541 aaccattatc aaggcggccg cagacctgat ctcccgtgat gcgccggatt atcagtatct
      601 cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc tacggccagt ttgagccgcc
      661 tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc aaatacgata atcatctgct
      721 ggaagactac acggaagaag agttcaagca gatggacacg tttatcgatc acgaccgtga
      781 tatgaccttc tcttatgctg ccgttaagca gctggaaggc aaatatctgg tacagaaccg
      841 cgtgaccggc gaaatctacg aaagcgccca gttcctttat attctggttg ccgcgtgcct
      901 gttctcgaac tacccgcgtg aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt
      961 ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg
     1021 tcagttcagc tcctgcgtac tgatcgagtg cggtgacagt ctggattcca tcaacgctac
     1081 ctccagcgcg attgtgaaat acgtttccca gcgtgccggg atcggcatca acgccgggcg
     1141 tattcgtgcg ctgggtagcc cgattcgcgg tggtgaagcg ttccataccg gctgtattcc
     1201 gttctacaaa catttccaga cagcggtgaa atcctgctct cagggcggcg tgcgcggcgg
     1261 tgcggcaacg ctgttctacc cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa
     1321 aaacaaccgt ggtgtggaag gcaaccgcgt gcgccatatg gactacgggg tacagatcaa
     1381 caaactgatg tatacccgcc tgctgaaagg tgaagatatc accctgttca gcccgtccga
     1441 cgtaccgggg ctgtacgacg cattcttcgc cgatcaggaa gagtttgaac gcctgtatac
     1501 caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc
     1561 gctgatgatg caggaacgtg cgtctaccgg tcgtatctat attcagaacg ttgaccactg
     1621 caatacccat agcccgtttg atccggccat cgcgccagtg cgtcagtcta acctgtgcct
     1681 ggagatagcc ctgccgacca aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc
     1741 gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat agcctggatg aactggaaga
     1801 gctggcaatt ctggcggttc gtgcacttga cgcgctgctg gattatcagg attacccgat
     1861 cccggccgcc aaacgtggag cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt
     1921 cgcttactac ctggcgaagc acggtgtgcg ttactccgac ggcagcgcca acaacctgac
     1981 gcataaaacc ttcgaagcga ttcagtatta cctgctgaaa gcgtctaacg agctggcaaa
     2041 agagcaaggc gcgtgcccgt ggtttaacga aaccacttac gcgaaaggga tcctgccgat
     2101 cgatacctat aagaaagatc tggataccat cgctaatgag ccgctgcatt acgactggga
     2161 agcattgcgt gaatcaatca aaacgcacgg cctgcgtaac tccacgcttt ctgccctgat
     2221 gccgtcagag acttcttcac agatctctaa cgccactaac ggtattgaac cgccgcgcgg
     2281 ttacgtcagc atcaaagcgt cgaaagacgg tattttgcgc caggtggtgc cggactacga
     2341 gcacctgcac gacgcctatg agctgctgtg ggaaatgccg ggtaacgatg gctacctgca
     2401 actggtaggt atcatgcaga aatttatcga tcagtcgatc tctgctaaca ccaactacga
     2461 tccgtcacgt ttcccgtcag gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac
     2521 cgcctacaaa ttcggggtca aaacgctgta ttatcagaac acccgtgacg gcgctgaaga
     2581 cgcgcaggac gatctggtac cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa
     2641 gatctgatat tgagatgccg gatgcagcgt gaacgcctta tccggcctac ggctcggatt
     2701 tgtaggcctg ataagacgcg tcagcgtcgc atcaggcaca ggatgcggcg taaaatgcct
     2761 tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt ttcacagacg
     2821 aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt ggctcgctac
     2881 gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc tttcttctgg
     2941 cgtccggaag aagttgacgt ttctcgcgac cgtatcgatt accaggcact gcctgaacac
     3001 gaaaaacaca tctttatcag caacctgaaa tatcagacgc tgctggattc cattcagggc
     3061 cgtagcccga acgtggcact gctgccgctg atctcgatcc cggaactgga gacctgggtc
     3121 gaaacctggg cgttctcaga aacgattcat tcccgttcct atactcatat cattcgtaat
     3181 atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca gatccagaaa
     3241 cgtgcggaag ggatctccag ctattacgat gagctgatcg aaatgaccag ctactggcat
     3301 ctgctgggcg aaggtaccca caccgttaac ggtaaaactg tgaccgttag cctgcgcgag
     3361 ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc gattcgtttc
     3421 tacgtcagct ttgcttgttc cttcgcattt gcagaacgcg aattgatgga aggcaacgcc
     3481 aaaattattc gcctgattgc ccgcgacgaa gccctgcacc tgaccggcac ccagcatatg
     3541 ctgaatctgc tgcgcagcgg cgcggacgat cctgagatgg cggaaattgc cgaagagtgt
     3601 aagcaggagt gctatgacct gtttgttcag gcagctcaac aggagaaaga ctgggcggat
     3661 tatctgttcc gcgacggttc gatgattggt ctgaataaag acattctctg ccagtacgtt
     3721 gaatacatca ccaatatccg tatgcaggca gtcggtttgg atctgccgtt ccagacgcgc
     3781 tccaacccga tcccatggat caacacctgg ctggtgtctg ataacgtgca ggttgctccg
     3841 caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt ggacaccgac
     3901 gatttgagta acttccaact ctgatggccc gcgttaccct gcgcatcact ggcacacaac
     3961 tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac aatgtggcgg
     4021 ttgagtatca gtgtcgcgaa ggttactgcg gctcctgtcg cacccgtctg gttgcaggtc
     4081 aggttgactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt ttgccctgtt
     4141 gttgccgggc aaaaggcgat attgaaatcg agatgtgaat tggttgtagt gccagataca
     4201 acgcttatgc gtcttatctg gcctacaacg attacatggc gtagtaatac gctttcacct
     4261 gctcccagtc cgctttgggg attggctcca gatatttttc cagctggcgg aagtcatgat
     4321 taatcgcttt atcgcgacgc aagcggcgac gacttttctc caggtcaaga aaaccagctt
     4381 ctgcattacc ttctgttttc acatagatat ggcgaacata acaacagcca tgctgacgat
     4441 taatgctatg cattttctta aacgccagcg ccactgcttt caacatggct tgccgtactt
     4501 cgtcagaata aggcgatact gcatgctggg cataccagtc agcaatgctg atgaaccccg
     4561 ccatatcttc agtcaccagc aacgctcgcc attcaccctc aattttcacc gcttcaccaa
     4621 aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc gcaacctcac
     4681 ggacaatcgt tggtcggccg aacggataac gtacggaatg aaacagatga tgcgtcatgc
     4741 gctttacata cagctttttg ccgttgcgct cgacgcattg caccccgctc ataccattac
     4801 ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga ttaaattcgt
     4861 cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg acaatgacta
     4921 taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attaatgaat ttcttttgcc
     4981 actactagct tgacaccgct tttacccttc atttgcagcg catagattcc accagccact
     5041 accactaata gtgcgcccag cagataaaat gtgaatttaa tcaggcggat acagcgatga
     5101 attttattcg acagggatta ggcatcgcct tacaaccaga gttaacgctg aaaagcattg
     5161 caggtgaatt gtgttccgtt cctctcgaac caactttcta tcgacagatt tcgttgctgg
     5221 ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaatgtgc atggaacaat
     5281 tagtggcgat tggaaaaatt tgatatgaga caggatggcg catgaacgcc atcctgcagt
     5341 a
//
LOCUS       DAZFAH010000261         5278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFAH010000261 DAZFAH010000000
VERSION     DAZFAH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.263"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            206..1540
                     /gene="cadB"
                     /locus_tag="ACHJWB_004460"
     CDS             206..1540
                     /gene="cadB"
                     /locus_tag="ACHJWB_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4315959.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1620..3767
                     /gene="cadA"
                     /locus_tag="ACHJWB_004461"
     CDS             1620..3767
                     /gene="cadA"
                     /locus_tag="ACHJWB_004461"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4315960.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3826..>5278
                     /gene="dtpC"
                     /locus_tag="ACHJWB_004462"
     CDS             3826..>5278
                     /gene="dtpC"
                     /locus_tag="ACHJWB_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4315961.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESN"
BASE COUNT         1233 a         1274 c         1296 g         1475 t
ORIGIN      
        1 tcttttcatg atatcaactt gcgatcctga tgtgttaata aaaaacctca agttctcact
       61 tacagaaact tttgtgttat ttcacctaat ctttaggatt aatccttttt tcgtgagtat
      121 ccttagcgcc agtttggtct ggtcaggaaa tagttataca tcatgacccg gactccaaat
      181 tcaaaaatga aattaggaga agagcatgag ttctgccaag aagatcgggc tatttgcctg
      241 taccggtgtt gttgccggta atatgatggg gagcggtatt gcattattac ctgcgaacct
      301 agcaagtatc ggtggtattg ctatctgggg ttggattatc tctattattg gtgcaatgtc
      361 gctggcgtat gtatatgccc gactggcaac aaaaaacccg caacaaggtg gcccaattgc
      421 ttatgccgga gaaatttccc ctgcatttgg ttttcagaca ggtgttcttt attaccatgc
      481 taactggatt ggtaacctgg cgattggtat taccgcggta tcttatcttt ccaccttctt
      541 cccagtatta aatgatcctg ttccggcggg tatcgcctgt attgctatcg tctgggtatt
      601 tacctttgta aatatgctcg gcggtacttg ggtaagccgt ttaaccacta ttggtctggt
      661 gctggttctt attccggtag taatgaccgc tattgttggc tggcattggt ttgatgcggc
      721 aacttatgca gctaactgga atactgcgga taccactgat ggtcatgcga tcattaaaag
      781 tattctgctc tgcctgtggg ccttcgtggg tgttgaatcc gcagcagtaa gtactggtat
      841 ggttaaaaac ccgaaacgta ccgttccgct ggcaaccatg ctgggtactg gtttagcagg
      901 tattgtttac atcgctgcga ctcaggtgct ttccggtatg tatccatctt ctgtaatggc
      961 agcttccggt gctccgtttg caatcagtgc ttcaaccatc ctcggtaact gggctgcgcc
     1021 gctggtttct gcattcaccg cctttgcgtg cctgacttct ctgggctcct ggatgatgtt
     1081 ggtaggccag gcaggtgtac gtgccgctaa cgacggtaac ttcccgaaag tttatggtga
     1141 agtcgacagc aacggtattc cgaaaaaagg tctgctgctg gctgcagtga aaatgactgc
     1201 cctgatgatc ctcatcactc tgatgaactc tgccggtggt aaagcatctg acctgttcgg
     1261 tgaactgacc ggtatcgcag tactgctgac tatgctgccg tacttctact cttgcgttga
     1321 cctgattcgt tttgaaggcg ttaacatccg caactttgtc agcctgatct gctctgtact
     1381 gggttgtgtg ttctgcttca tcgcgctgat gggcgcaagc tccttcgagc tggcaggtac
     1441 cttcatcgtc agcctgatta tcctgatgtt ctacgctcgc aaaatgcacg agcgccagag
     1501 ccactcaatg gataaccaca ccgcgtctaa cgcacattaa ttaaaagtat tttccgaggc
     1561 tcctcctttc attttgtccc atgtgttggg aggggccttt tttacctgga gatatgacta
     1621 tgaacgttat tgcaatattg aatcacatgg gggtttattt taaagaagaa cccatccgtg
     1681 aacttcatcg cgcgcttgaa cgtctgaact tccagattgt ttacccgaac gaccgtgacg
     1741 acttattaaa actgatcgaa aacaatgcgc gtctgtgcgg cgttattttt gactgggata
     1801 aatataatct cgagctgtgc gaagaaatta gcaaaatgaa cgagaacctg ccgttgtacg
     1861 cgttcgctaa tacgtattcc actctcgatg taagcctgaa tgacctgcgt ttacagatta
     1921 gcttctttga atatgcgctg ggtgctgctg aagatattgc taataagatc aagcagacca
     1981 ctgacgaata tatcaacact attctgcctc cgctgactaa agcactgttt aaatatgttc
     2041 gtgaaggtaa atatactttc tgtactcctg gtcacatggg cggtactgca ttccagaaaa
     2101 gcccggtagg tagcctgttc tatgatttct ttggtccgaa taccatgaaa tctgatattt
     2161 ccatttcagt atctgaactg ggttctctgc tggatcacag tggtccacac aaagaagcag
     2221 aacagtatat cgctcgcgtc tttaacgcag accgcagcta catggtgacc aacggtactt
     2281 ccactgcgaa caaaattgtt ggcatgtact ctgctccggc aggcagcacc attctgattg
     2341 accgtaactg ccacaaatcg ctgacccacc tgatgatgat gagcgatgtt acgccaatct
     2401 atttccgccc gacccgtaac gcttacggta ttcttggtgg tatcccacag agtgaattcc
     2461 agcacgctac cattgctaag cgcgtgaaag aaacaccaaa cgcaacctgg ccggtacatg
     2521 ctgtaattac caactctacc tatgatggtc tgctgtacaa caccgacttc atcaagaaaa
     2581 cactggatgt gaaatccatc cactttgact ccgcgtgggt gccttacacc aacttctcac
     2641 cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa gtgatttacg
     2701 aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg atccacgtta
     2761 aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc accacttctc
     2821 cgcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa ggcaatgcag
     2881 gtaaacgtct gatcaacggt tccattgaac gtgcgatcaa attccgtaaa gagatcaaac
     2941 gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat catatcgata
     3001 cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa aacatcgata
     3061 acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg atggaaaaag
     3121 acggcaccat gagcgacttt ggtattccgg ccagcatcgt ggcgaaatac ctcgacgaac
     3181 atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc agcatcggta
     3241 tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa cgtgcgttcg
     3301 acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat cctgaattct
     3361 atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt gttcaccaca
     3421 atctgccgga tctgatgtac cgcgcatttg aagtgctgcc gacgatggta atgactccgt
     3481 atgctgcatt ccagaaagag ctgcacggta tgaccgaaga agtttacctc gacgaaatgg
     3541 taggtcgtat taacgccaat atgatccttc cgtatccgcc gggagttcct ctggtaatgc
     3601 cgggtgaaat gatcaccgaa gaaagccgtc cggttctgga gttcctgcag atgctgtgtg
     3661 aaatcggcgc tcactatccg ggctttgaaa ccgatattca cggtgcatac cgtcaggctg
     3721 atggccgcta taccgttaag gtattgaaag aagaaagcaa aaaataatta gctcgtacaa
     3781 gggaagtggc ttgccacttc ccttttttgc tcataaggag aacacatgaa aacaccctca
     3841 cagccgcgcg cgatatacta tatcgtggcg atccaaatct gggagtactt cagtttttac
     3901 ggcatgcgtg ccttactcat tctctatctc acccatcagc ttggttttga tgataaccat
     3961 gccatcagcc tgttcagcgc atatgcttct ctggtttacg ttacccctat tctcggcggc
     4021 tggcttgccg accgcctgct cggcaaccgc actgcagtga ttgccggcgc gctgttaatg
     4081 acccttggcc atgtggtgct gggcattgat acaaattcaa cctttagcct gtatctggcg
     4141 ctggcaatca ttatttgtgg ctacggttta ttcaaatcaa acatcagctg tttgcttggc
     4201 gagctttacg acgagaacga tcatcgacgt gatggcggtt tttcgctgct gtatgctgcg
     4261 ggcaatatcg gttctatcgc cgccccaatc gcctgcggcc tggctgctca gtggtatgga
     4321 tggcatgttg gctttgccct tgcgggtggc ggcatgttta tcggtttgtt gattttctta
     4381 agcgggcatc gtcatttcca gtccacgcgt agtatggata aaaaagcgct cactagcgtc
     4441 aaatttgcct taccggtatg gagctggtta gtggtgatgc tctgtttagc cccagtattt
     4501 tttaccctgc tgctggagaa cgactggtcc ggatatttgc tggcgatcgt ttgcctcatt
     4561 gccgcacaaa tcattgcccg catgatggtc aaattccccg aacaccgccg tgctctttgg
     4621 caaattgtat tgttgatgtt tgtcgggaca ttgttctggg tactggcaca acagggcggc
     4681 agtaccatca gcttgtttat cgatcgcttt gtgaatcgtc aggcattcaa tattgaagta
     4741 cctacagcac tattccagtc ggtgaatgca attgcggtga tgctcgctgg ggttgtactg
     4801 gcctggctgg cgtcgccaga aagccgcggc aactcaacat tgcgcgtctg gctgaagttt
     4861 gcctttggct tactactgat ggcttgtggc tttatgttgt tggcatttga tgcccgacat
     4921 gcagcggctg acggtcaagc gtcaatgggc gtgatgatat ccgggttggc gctaatgggc
     4981 tttgccgaac tctttattga cccggtggcg attgcgcaaa tcacgcgtct gaaaatgtct
     5041 ggcgtattaa ccggtattta tatgctggca acaggcgcgg tcgccaactg gctggcaggc
     5101 gtcgtggcac agcagacgac agagtcgcaa attagcggta tggcaattgc agcttaccag
     5161 cgattctttt ctcagatggg agagtggacg ttggcttgtg tcgcgatcat cgtcgtattg
     5221 gcctttgcta cccgttttct gtttagcacg ccgacgaata tgatacagga gagcaacg
//
LOCUS       DAZFAH010000262         5223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFAH010000262 DAZFAH010000000
VERSION     DAZFAH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.264"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..357
                     /gene="lpfD-O113"
                     /locus_tag="ACHJWB_004463"
     CDS             <1..357
                     /gene="lpfD-O113"
                     /locus_tag="ACHJWB_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO4315962.1"
                     /translation="GSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMV
                     VTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPA
                     PGQFTATATITVEIVR"
     gene            605..1645
                     /gene="pstS"
                     /locus_tag="ACHJWB_004464"
     CDS             605..1645
                     /gene="pstS"
                     /locus_tag="ACHJWB_004464"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4315963.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVDQVRAAWK
                     TNIKDSSGKPLY"
     gene            1732..2691
                     /gene="pstC"
                     /locus_tag="ACHJWB_004465"
     CDS             1732..2691
                     /gene="pstC"
                     /locus_tag="ACHJWB_004465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4315964.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2691..3581
                     /gene="pstA"
                     /locus_tag="ACHJWB_004466"
     CDS             2691..3581
                     /gene="pstA"
                     /locus_tag="ACHJWB_004466"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4315965.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3672..4445
                     /gene="pstB"
                     /locus_tag="ACHJWB_004467"
     CDS             3672..4445
                     /gene="pstB"
                     /locus_tag="ACHJWB_004467"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4315966.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4460..5185
                     /gene="phoU"
                     /locus_tag="ACHJWB_004468"
     CDS             4460..5185
                     /gene="phoU"
                     /locus_tag="ACHJWB_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4315967.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1245 a         1295 c         1411 g         1272 t
ORIGIN      
        1 ggaagtgcgg taacaacaca taaaattact aaaacggtca ctattgagtg tgaaaacctg
       61 gggatactga ataccgatga tatcagtacg tctttttatg ctaccgaacc caatacggac
      121 aattcaatgg tcgtaacttc aaactctaac gtggggataa aaatttacga caaaaataat
      181 aaggaaatca aagtgaacgg tggtgagttg ccaacagaca tgggtaaatc aacagtgtat
      241 ggtgaaaaat caggtagtgt aactttttca gctgctcctg cgagccttac tggagctcgc
      301 ccagccccgg ggcaatttac tgcaacagcg acgataactg ttgagattgt gcgctaatag
      361 gtctgacaac gattgttcat cacatcgttg tcatctcacc catttcttta tcacttttaa
      421 atgcatcatg acaaaaagat gacatgtggg attttcatct tcacatttaa aatcagtaag
      481 ttatttggta attcctttct gtcataaaaa tgtcatattc cttacatata actgtcacct
      541 gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc tcccaggaga
      601 cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct tatcgatgag
      661 cgctttctct gtgtttgcag aagcaagcct gacaggtgca ggtgcaacct tccctgcgcc
      721 ggtgtatgcc aaatgggctg acacttacca gaaagaaacc ggtaataaag ttaactacca
      781 gggtatcggt tcttccggtg gcgtaaaaca gattatcgct aataccgttg attttggtgc
      841 ctctgacgcg ccgctgtctg atgaaaaatt ggctcaggaa ggcctgttcc agttcccgac
      901 cgtgattggc ggcgtggtgc tggctgttaa cattccaggg ctgaagtctg gcgaactggt
      961 gctggatggt aaaaccctcg gcgacatcta cctgggcaaa atcaagaagt gggatgatga
     1021 agccatcgcc aaactgaatc cgggtctgaa actgccttca cagaacattg ctgtagtacg
     1081 ccgcgcagat ggctccggga cttccttcgt cttcaccagc tacctggcga aagtgaacga
     1141 agagtggaaa aacaacgttg gtactggctc taccgtaaaa tggccgatcg gtctgggcgg
     1201 taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa ttggttacgt
     1261 tgaatatgct tacgcgaagc agaacaacct ggcgtatacc aaattgatct ccgctgatgg
     1321 taaaccggtt agtccgaccg aagaaaactt cgctaatgca gcaaaaggcg cagactggag
     1381 caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcgtggc ctattacctc
     1441 taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca cagaagtgct
     1501 gaaattcttc gactgggcgt acaaaaccgg ggctaaacag gcgaacgacc tggattacgc
     1561 cagcctgccg gatagcgtag ttgaccaggt tcgcgctgcg tggaagacca atattaaaga
     1621 cagtagcggt aagccgctgt actaataaaa ctccaggccg ggtacggtgt tttacaccgc
     1681 atccggcatt acaaaataac tttgtaaacg cgtttaactg aagagtaact tatggctgca
     1741 accaagcctg cttttaaccc accgggtaaa aagggcgaca taattttcag cgtgctggta
     1801 aaactggcgg cgctgattgt gctattgatg ttgggtggca ttattgtctc tctgatcatc
     1861 tcctcctggc cgagcattca gaaatttggt ctggcttttc tgtggaccaa agagtgggat
     1921 gcaccgaacg atatctacgg ggcgctggtg ccgatctacg gtacgctggt gacttcgttt
     1981 atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg ccctgttcct gactgagctt
     2041 gcgcctggct ggctgaaacg cccgctgggt atcgccattg agctgctggc agccattcca
     2101 agtatcgttt acggcatgtg gggcctgttt atctttgcgc cgctgttcgc cgtttacttc
     2161 caggagccgg tcggcaatat catgtcgaat atcccgattg ttggcgcgct gttctctggc
     2221 cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc tcgccatcat gattattccg
     2281 tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc cggtgatgat gaaagagtcg
     2341 gcctacggta ttggctgcac cacctgggaa gttatctggc gtatcgttct tccgttcacc
     2401 aaaaatggtg ttatcggcgg catcatgctg gggctgggcc gcgcgctcgg tgaaaccatg
     2461 gcggtgacct ttatcatcgg taacacctac cagctcgaca gcgcctcgct gtatatgccg
     2521 ggcaacagta tcacctctgc gctggcgaac gaatttgcgg aagcggaatc cggtctgcac
     2581 gttgccgcac tgatggaact gggcctgatc ctgtttgtga ttaccttcat cgtcctcgcc
     2641 gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta atggctatgg
     2701 ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag gcgcgtcgcc
     2761 gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc ggcctgttct
     2821 ggctgatctg gattttaatg tccaccatca ctcgcggtat cgacggtatg tcgctggcgc
     2881 tgttcactga aatgacgccg ccgcccaata cggaaggtgg tggtctggcg aacgccctgg
     2941 cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg tacgccgctg ggcattatgg
     3001 cggggattta tctggcggaa tatggtcgta aatcctggct ggcagaagtg attcgcttca
     3061 ttaacgacat tctgctctcc gcgccgtcga ttgtggttgg tctgtttgtt tacaccatcg
     3121 tggtggcgca gatggagcac ttctccggct gggcgggcgt gattgccctg gcgctgttgc
     3181 aggtgccgat tgttatccgc accaccgaga acatgctgaa actggtgccg gacagcctgc
     3241 gtgaagcggc ttatgcgctg ggtacaccga agtggaagat gatctccgcg attacgctga
     3301 aagcgtcggt gtccgggatc atgaccggta tcttgctggc gattgcccgt attgctggtg
     3361 aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt ctggagcacg gacatgatgc
     3421 agccgatcgc caacctgccg gtgacgatct ttaagtttgc gatgagtccg tttgcggaat
     3481 ggcagcaact ggcctgggcc ggggtattga tcattaccct gtgcgtactg ctgctgaaca
     3541 ttctggcgcg cgttgttttt gcgaagaata aacacggtta acactttgcc ggatgcggcg
     3601 tgaacgcctg atccggccta cggtaagcct gattcgcgaa gtgcatcagg cacgatgagg
     3661 aaaagattgc aatgagtatg gttgaaactg ccccgagtaa aattcaggtt cgtaatttga
     3721 acttctacta cggcaaattc catgccctga aaaacatcaa cctggatatc gctaaaaacc
     3781 aggttacggc gtttatcggg ccgtccggct gcggtaaatc gacgctgctg cgtaccttca
     3841 acaaaatgtt tgaactgtac ccggagcagc gtgcggaagg tgaaattctg cttgatggcg
     3901 acaacatcct gaccaactct caggatatcg ccctgctgcg tgcgaaagtg ggcatggtgt
     3961 tccagaaacc gacgccgttc ccgatgtcca tctacgacaa cattgccttt ggcgttcgtc
     4021 tgtttgagaa gctctcccgt gccgacatgg acgagcgcgt gcagtgggca ttgaccaaag
     4081 ccgcattgtg gaacgaaacc aaagataaat tgcaccagag cggttactct ctctctggtg
     4141 gtcagcaaca gcgtctgtgc attgcgcgtg gtatcgccat tcgcccggaa gtgctgctgc
     4201 tcgacgaacc gtgttcggcg ctcgacccca tctctaccgg gcgtattgaa gagctgatca
     4261 ccgaactgaa gcaggattac accgtggtga tcgtcaccca caacatgcag caggctgcgc
     4321 gttgttccga ccacacggcg tttatgtacc tgggcgaatt gattgagttc agcaacacgg
     4381 acgatctgtt caccaagcca gcgaagaaac aaacagaaga ctacatcacc ggtcgttacg
     4441 gttgattcag gagtgcgtta tggacagtct caatcttaat aaacatattt ccggccagtt
     4501 caacgccgaa ctggaaagta tccgcacgca ggtgatgacc atgggcggca tggtggagca
     4561 gcagctttct gatgcaatca ccgcgatgca taaccaggac agcgatctgg cgaagcgcgt
     4621 gatcgaaggc gacaagaacg tcaacatgat ggaagtggcg atcgatgaag cctgcgtgcg
     4681 cattatcgcc aaacgtcagc cgacggcgag cgacctgcga ctggttatgg tgatcagtaa
     4741 aaccattgcc gagctggagc gtattggcga cgtggcggac aaaatctgcc gtactgcgct
     4801 ggagaaattc tcccagcagc atcagccgtt gctggtaagt ctggagtcgc tgggccgtca
     4861 taccatccag atgctgcacg acgtgctgga cgcgttcgcg cggatggaca ttgacgaagc
     4921 ggtacgtatt tatcgtgaag ataaaaaagt cgatcaggaa tacgaaggta ttgttcgtca
     4981 actgatgacc tacatgatgg aagattcgcg taccattccg agcgtactta ctgcgctgtt
     5041 ctgcgcgcgt tctatcgaac gtattggcga ccgctgccag aatatttgtg agtttatctt
     5101 ctactacgtg aaggggcagg atttccgtca cgtcggtggc gatgagctgg ataaactgct
     5161 ggcggggaaa gatagcgaca aataattcac cagacaaatc ccaataactt aattattggg
     5221 att
//
LOCUS       DAZFAH010000263         5219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFAH010000263 DAZFAH010000000
VERSION     DAZFAH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.265"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(108..590)
                     /gene="ybiA"
                     /locus_tag="ACHJWB_004469"
     CDS             complement(108..590)
                     /gene="ybiA"
                     /locus_tag="ACHJWB_004469"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO4315968.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            710..2860
                     /gene="dinG"
                     /locus_tag="ACHJWB_004470"
     CDS             710..2860
                     /gene="dinG"
                     /locus_tag="ACHJWB_004470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4315969.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLISGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILDLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2888..3850
                     /gene="ybiB"
                     /locus_tag="ACHJWB_004471"
     CDS             2888..3850
                     /gene="ybiB"
                     /locus_tag="ACHJWB_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4315970.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3991..5076
                     /gene="hcxB"
                     /locus_tag="ACHJWB_004472"
     CDS             3991..5076
                     /gene="hcxB"
                     /locus_tag="ACHJWB_004472"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4315971.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQGFCQQLAS"
     gene            5142..5201
                     /gene="ybiE"
                     /locus_tag="ACHJWB_004473"
     CDS             5142..5201
                     /gene="ybiE"
                     /locus_tag="ACHJWB_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4315972.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
BASE COUNT         1231 a         1314 c         1474 g         1200 t
ORIGIN      
        1 gataaggcgt ttacgccgca tccggcattt cacaacaagc acttgtcagc aatttgagaa
       61 cacgggaaaa aatttatctg ttttaccgcc cataagccac cgagacgtta cttctctata
      121 gccaattgtt cgcgcaactc cattaaaagg tagcccagtc gattcttgcc cttaccatta
      181 ccaccgtctc cccagtaagc atcgttttcc gtatgctcaa ccagttttgc gggcgcggtt
      241 gccagcaaga gcgcacgcag ttctgcatgc tgttcgaatt tagcgcgaag tgcttttcgc
      301 atcacctgtt ctttgaccga ctcccagttt ttacgcagag gcttagaacg atcgcgcccc
      361 atgcgtgcgg cgaccatagg ggaagaaacc cgacggatct cttcgcggta tttttcatca
      421 aggaattttt gtgcctgaaa atagtgttct gaggtgggcc aggttttccc gtcaaccttg
      481 atgggccagg cggcaaagtt agaaaaatca ccgtagtcat cgctggtgct gtagaaattt
      541 atgatggtgt cttgcatgac gtgttggatc ctttgtgctc gaacgggcat taaaccgcat
      601 tatgttggtg gttattgcga gccgctttcc agaaacagaa aaaccattac ccctgaaaac
      661 cgaaaaatgc cacaatattg gctgtttata cagtatttca ggttttctca tggcattaac
      721 cgccgcgctt aaagcgcaaa ttgccgcctg gtataaggcg cttcaggaac agatccccga
      781 ctttattccc cgtgcgccgc agcggcagat gattgcggac gtcgccaaaa cgctggccgg
      841 agaagaaggg cgacatctgg cgattgaagc ccccaccggc gttgggaaaa cgctctctta
      901 tttgatttcc ggcatcgcca ttgcccgcga agagcaaaaa acgctggtgg tgagtaccgc
      961 caacgtggca ttgcaggatc agatttacag caaagattta ccgctgctga aaaagatcat
     1021 tcccgatctt aaattcactg ccgcttttgg gcgtgggcgc tacgtttgtc cgcgtaatct
     1081 gacggcgctc gccagtactg aacccacgca acaggatctg ctggcgtttc ttgacgacga
     1141 actgacgccg aacaatcagg aagagcaaaa acgttgtgcg aagctgaagg gcgatctcga
     1201 tacttataaa tgggatggtc tgcgcgatca tacggatatc gctataaatg acgatctctg
     1261 gcgtcgtttg agtaccgaca aagccagctg cctcaaccgt aactgttact actatcgtga
     1321 atgcccgttt tttgttgctc gtcgggagat tcaggaagcg gaagtggtgg tggcaaacca
     1381 tgcgctggtg atggcggcga tggaaagcga agcggtattg cctgacccga aaaatttact
     1441 gctggtgctg gacgaaggtc atcacctgcc ggatgtggcg cgggatgcgc tggagatgag
     1501 cgccgaaatc accgcaccct ggtatcgact acagctggac ttgttcacga aactggtcgc
     1561 tacctgcatg gagcagtttc gcccgaagac catcccgccg ctggcgatcc ctgaacgttt
     1621 gaatgcgcat tgtgaagagt tgtatgagct tatcgcctcg ttaaacaaca ttctcgatct
     1681 ctacatgcct gccgggcagg aggcagagca ccgttttgcg atgggcgaac tgccagatga
     1741 agtgctggag atctgccagc ggctggcaaa actcaccgag atgctgcgtg gtctggcgga
     1801 gttatttctt aacgatttaa gtgagaaaac cggcagccat gacattgtac gcctgcatcg
     1861 gttgattttg cagatgaacc gcgcgttggg gatgttcgag gcgcaaagca aactctggcg
     1921 gctggcgtct ctggcgcaat cttccggtgc accggtgacc aaatgggcga cgcgggaaga
     1981 gcgcgaaggg cagctgcatc tctggtttca ctgcgtggga atacgtgtca gcgatcagct
     2041 ggaaaggctg ctgtggcgca gtattccgca cattattgtc acctccgcaa ccttgcgttc
     2101 gctgaacagt ttttcgcgtt tgcaggagat gagtggtctg aaagagaaag cgggcgaccg
     2161 ttttgtggcg ctggattccc cctttaacca ctgcgaacag ggcaaaattg ttattccccg
     2221 gatgcgcttt gagccttcca tcgacaacga agagcagcat attgccgaaa tggcggcctt
     2281 tttccgtaag caggtggaga gcaaaaaaca tctcggtatg ttggtactgt ttgccagcgg
     2341 acgggcgatg cagcgctttc tcgactatgt gacggattta cgcctgatgc tgctggtgca
     2401 gggcgatcag ccgcgttatc gcttagttga actgcaccgc aaacgcgttg ccaacggtga
     2461 gcgtagtgtg ctggtgggct tacagtcatt tgccgaaggg cttgatttga aaggtgatct
     2521 gctcagccag gtgcatatcc acaaaatcgc ttttccgccc atcgacagcc cggtggtgat
     2581 caccgaaggg gaatggctga aaagcctcaa ccgctatccg tttgaggtgc aaagcctgcc
     2641 gagcgcctcg tttaacctga ttcagcaggt tgggcgactg attcgaagcc acggttgctg
     2701 gggcgaagtg gttatctacg acaaacgctt gctgaccaaa aattatggca agcgactact
     2761 ggatgcatta ccggtatttc cgatagagca accggaagtc cctgaaggta tagttaaaaa
     2821 gaaagaaaaa acgaaatccc cacgccgtcg gcggcgttaa tgatgtgagt caggtaagga
     2881 gtcgtaaatg gactatcgca aaatcattaa agagatcggg cgcgggaaaa accacgcgcg
     2941 cgatttagac cgggatactg cgcgcggtct gtatgcccat atgctcaatg gtgaagtccc
     3001 tgaccttgag ttgggcggcg tattgattgc gctgcgtatc aaaggggaag gggaagcaga
     3061 gatgctcggc ttttacgaag ccatgcaaaa tcacaccatc aagctgacgc cgcctgcagg
     3121 caagccgatg ccgattgtca tccccagtta caacggcgcg cgtaaacagg ccaacctgac
     3181 gccgttgttg gcgattctcc tgcacaaact cggttttcct gtggtggttc acggggttag
     3241 cgaagatcca acccgcgtgc tgactgaaac catttttgaa ttgatgggta ttacgccaac
     3301 gttgcacggc gggcaggcgc aggcgaagct cgacgaacat caaccggtgt ttatgccagt
     3361 cggcgcgttt tgcccgccgc tggaaaaaca actggcgatg cgctggcgga tgggcgtgcg
     3421 taacagtgca cataccctgg cgaaactggc aacgccattt gccgaaggtg aggcgctgcg
     3481 tctttccagc gtttcgcatc cggaatacat tggacgcgtc gcgaagttct ttagtgatat
     3541 cggcgggcgg gcgctactaa tgcatggtac tgaaggtgaa gtgtatgcta atccgcagcg
     3601 ctgcccgcag atcaatctca ttgaccgtga agggatgcgg gtgctgtatg aaaaacagga
     3661 cactgctggt agcgagttac tgccacaagc aaaagatccg gaaaccacgg cgcagtggat
     3721 tgagcgttgc cttgctggca gcgaaccgat tcccgaatcg ctgaaaatcc agatggcttg
     3781 ctgcctggtg gctacgggtg aagcggcaac tatcagcgac ggcctggcgc gcgttaatca
     3841 ggcattttaa ttcttttctc agccggatga gccatgtcat ccggctttta ccccaccgca
     3901 atatgaaatt cctgcatctt tattgacctt cccacgcccg gcgtgcagca taaaaataca
     3961 acaaacacat aacataaaca ggagttaacc atggaaagtg gtcatcgctt tgatgctcag
     4021 acgctgcaca gttttattca ggctgtattt cgtcagatgg gtagcgagga acaagaagcg
     4081 aaattagttg ccgatcattt aatcgcggca aacctggcag ggcatgattc acatggtatt
     4141 ggcatgatcc caagctatgt acgctcctgg agtcaggggc acctgcaaat taaccatcat
     4201 gccaaaaccg ttaaagagtc gggggcggcg gtcacgctcg atggcgatcg cgcatttggt
     4261 caggtcgcgg cacatgaagc gatggcgctg gggattgaga aagcgcatca gcacggtatt
     4321 gccgccgtgg cgctacataa ctcgcatcat atcggccgta tcggttactg ggcggagcag
     4381 tgtgcagcgg cggggtttgt ctctatccac tttgttagcg tggtcggtat tccaatggtc
     4441 gcgccgttcc acggtcgcga cagccgcttt ggcaccaatc ccttctgtgt ggttttccct
     4501 cgtaaagata atttcccgct gttgcttgat tacgccacca gcgccattgc atttggcaaa
     4561 acccgcgtcg cctggcataa aggcgtcccc gtgccgccag gttgcctgat tgacgttaac
     4621 ggcgtgccga cgaccaatcc ggcggtaatg caggagtcgc cgttgggttc gctgttgacc
     4681 tttgccgaac ataaaggcta cgcccttgca gcgatgtgtg aaattcttgg cggggcgctt
     4741 tccggcggta aaacgacgca tcaggaaacg ttacaaacca gtcccgatgc cattcttaac
     4801 tgcatgacca ctatcatcat caacccggaa ctcttcggcg cgccggattg tagcgcgcag
     4861 accgaagcct ttgccgagtg ggtgaaagcc tcgccgcatg atgatgataa gccgattttg
     4921 ctaccgggcg agtgggaagt gaacacgcgt cgcgaacggc aggagcaggg gattccattg
     4981 gatgcgggaa gctggcaggc catttgtgat gcggcgcggc agattggtat gtcggaagag
     5041 acgttgcagg gtttctgtca gcagttagcc agctaaaaaa agcccgtcca gtaacggacg
     5101 ggcaaacaag ggtaatatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
     5161 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcgga
//
LOCUS       DAZFAH010000264         5192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFAH010000264 DAZFAH010000000
VERSION     DAZFAH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.266"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..813
                     /gene="nanM"
                     /locus_tag="ACHJWB_004474"
     CDS             <1..813
                     /gene="nanM"
                     /locus_tag="ACHJWB_004474"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4315973.1"
                     /translation="GLTQVFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYV
                     TGGVNQNIFNGYFEDLNEAGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWS
                     YAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSS
                     PDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTNIHLWHNGKW
                     DKSGELSQGRAYGVSLPWNNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            878..1828
                     /gene="nanS"
                     /locus_tag="ACHJWB_004475"
     CDS             878..1828
                     /gene="nanS"
                     /locus_tag="ACHJWB_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4315974.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSEQHGASHDACRWGTDTPLYHDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFV"
     gene            complement(1866..1970)
                     /locus_tag="ACHJWB_004476"
                     /pseudo
     CDS             complement(1866..1970)
                     /locus_tag="ACHJWB_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2070..2942
                     /locus_tag="ACHJWB_004477"
     CDS             2070..2942
                     /locus_tag="ACHJWB_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO4315975.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEEHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            3112..5187
                     /locus_tag="ACHJWB_004478"
     CDS             3112..5187
                     /locus_tag="ACHJWB_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4315976.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
BASE COUNT         1503 a         1155 c         1215 g         1319 t
ORIGIN      
        1 ggcttgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt
       61 aaattgatgt cgcacgcacc gatgggcatg gcaggccatg tgacttttgt acacaacggc
      121 aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc
      181 aacgaggctg gaaaagattc aaccgctgta gataaaatca acgctcacta ttttgacaaa
      241 aaagcagaag attatttctt caataagttt ctgttgtctt ttgatccctc aacacagcaa
      301 tggagttacg ctggcgaatc tccctggtac ggaacggctg gtgcggcggt tgtgaataaa
      361 ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta
      421 tttgaacttg atttcaccgg taataattta aaatggaata agcttgctcc cgtctcatca
      481 ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg attctcttat atttgccgga
      541 ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa
      601 ggcctgaaaa aatcatatag cactaatatt catctttggc ataacgggaa atgggataaa
      661 tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagtcta
      721 ttgattattg gcggtgaatc tgcaggcggc aaagcggtga cggattcagt tttaatctct
      781 gtgaaggata ataaagtcac agtacaaaac taacgcttca gggccccagt aaaggggccc
      841 tgctatcaat ttgtcatttc aattacgcga aaattatatg gacgcaataa tatcgccaga
      901 ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg gcgaaggact
      961 gccattacca gacagggaag atgcgcctca tcccagaatt aaacaattag cgagatttgc
     1021 gcatacgcat cccggaggcc cgtcatgtca ctttaacgac attattccac tgactcactg
     1081 cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac
     1141 acagtacggc accgttggcc aggcattgca tattgcacgg aaattactgc cctttattcc
     1201 tgataatgca ggggttctca tcgttccatg ttgccgtggc ggatcggctt ttaccgcagg
     1261 cagcgaaggg acatattcag aacagcacgg agccagccat gatgcttgtc gttggggaac
     1321 ggatactccg ctgtatcatg atttagtcag cagaacgcga gccgcactgg caaaaaatcc
     1381 gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga
     1441 ctacgcgtca caccctcaac actttaatca tatgattgaa gcctttcgta gggatctaaa
     1501 acaataccat tctcagctta ataatattac tgacgcgccg tggttttgcg gcgataccac
     1561 ctggtactgg aaagaaaatt tccctcatgc gtatgaagtt atttatggca attatcaaaa
     1621 taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac
     1681 gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg
     1741 gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcgcgg cagcccgtcg
     1801 ggggattatt tctgacaggt ttgtataagc aattttgcag ttttggggcg aaaagtgagc
     1861 gttcattaat ttgatttatt cgcatccatc aacgctttta actcctccgg cgtaaacggt
     1921 ggtttgcgtc ggtgcagcat cgcatggcaa ttagggtaca caggcagcaa aaaaacaaat
     1981 gagatcaaag taatatatct tcacttgcag gcttgaacag ccgaaaattt gcgagcatga
     2041 tcgcaattat ttcactgcca gggatatcaa tggcttccag taaatcgcta cagcaggcaa
     2101 ttgccaatat aaaaatctgg cacaagggtg aacagcgcgc gccgcataag ccattgttat
     2161 tgctatacgt attagcggga tacctgaatg gacatccacg ccttttcgat tatggatcgg
     2221 aaatctacga acctctgcac agtttactgg aacgttttgg gccacagcgt tcacagtacc
     2281 gacctgatat gccgttctgg cgattacaag gcgacggatt ctggcaatta cacaatgccg
     2341 agctttgctc aacagcaggg agcagccgac aaccgccagt gaaggaactg aacgaatacc
     2401 acgttgcagg cggtttcgat gaacagcact acgccctggt aaccggcaat aaaaaactca
     2461 tcaatactct ggcgcaacag atactcgaag agcacttcac cgagagtatt caggaagaaa
     2521 ttgctgacga gctgggtttt gatctccagc aaatccgtaa acagcgcgac ccgttatttc
     2581 gcaaaaatgt attacgcgct tataactacc agtgcgcgat atgtggcttt aatatgcgcc
     2641 acgacgacac caccgtcgcg ctggaagcgg cgcatataaa atggaaacag cacggcggtc
     2701 cttgtgaaat tcctaatggc ctggcgcttt gcgcaattca ccataaagcc tttgataaag
     2761 gatcgattgg cctggacgaa aatatgcggg tactggtatc agatgcggta aacggcggag
     2821 ggatcgttga gagattattc tgggattttg acgggaaaac tattgcgttg ccgcaggtgc
     2881 gtaaaaatta cccttatgag gtgtttgtag aatggcatcg gaaagaggtt tttagggggt
     2941 gaggttgata acagcattca ccctacaacc ttacattgta tttaaattag agctgagtta
     3001 acagatagtt gtatatataa caggcacttg tcacaacgat taactcaata tagttagaat
     3061 acctcatatt aattaaaaaa taagtaatgt cacaacagag gaaaagtcac gatgactctt
     3121 gtagatagtg tcgaagcagg taggctaacg attagtgaat tgattgatgc cctggcgaaa
     3181 gataaaaatt acacagcttc cagatggtat cagcgatacc gtgcatttac gactttgcta
     3241 cagcaaacct caacttttgc tgagcctgca acagatggtc tggtcaaaca gctttggtat
     3301 gagcgtgaca acggcattgc aagtattcgc cagggcgttc catccttagc agaatatcag
     3361 caaagcctgc cactgcttag agaactaact gaacgaattc ggcaacagcc ggatgaagaa
     3421 acttaccaat atgttggcaa tgcacttcaa caagctaaag aaaacggact tctcaagcgt
     3481 atgtattgga gtttgagaaa tcgcgtcttt gccgcgttct cgccagaaaa ctacaccagt
     3541 actgtggatg agaatgcttt taataaagca gcagaattct taaatcagca cttccatctc
     3601 ggtttggtac tgaccggaaa ttggttacag aaaaactatg aattgaaaca agccatacac
     3661 gcccaatctc ctgatacaga tccttattat gtgaatatgg ccatctggca tctctatgaa
     3721 ttgctccgtg aacgcgataa tgaacaaaag caggagaaag tagctagcac tacatccata
     3781 acccgcagtg agcccatcga gaacaagatc atcctacatt caccaactaa cgtgatcttc
     3841 tttggccccc ctggcactgg caagaccttc aggttgcagc aaaaaatgaa agagtacact
     3901 tctcatgctg ttcccgctga tcgtgatgcc tggctggatt ctcgccttga atcgttgaac
     3961 tggatgcagg ttataacgct ggtgctgctc gatcttggga aacgagcgaa agttcgccaa
     4021 attattgaac atatgtggtt tcaacgtaag gcattattaa acggtcgtaa tggcaatcta
     4081 tcgaatactg cctgggcagc tttgcaatcc tatacagttc ccgagtcgtt aaccgttgat
     4141 tataagaatc ggcgtgagcc tgccgtattt aacaaaacag ataacagcga atggtttcta
     4201 gttgattcac agctcgagca agtggaggat ttggtagagc tctacgccga acttaaacgt
     4261 ggccctaaat ctgccgaagc catccagcgt tttgcggtgg ttacgttcca ccaatcttac
     4321 ggctatgaag aatttattga aggtatacgc gctcgctctg acgagagtgg caatatctct
     4381 tatcccattg agccgggtat ctttatgcgc ctttgccaac gtgcgaatgc cgatccagga
     4441 catcgctacg ccattttcat tgatgagatc aatcgcggta acatatccaa gatctttggt
     4501 gaactaatct cactcattga agtagacaag cgtgcaggca tgcccaatgc gatgagcctg
     4561 caactggctt atagcggtga tcacttcagc gtacccggca atgtcgatat catcggagcc
     4621 atgaatacag cggaccgttc tttagctctg atggacacgg ctttgcgccg tcgctttgac
     4681 tttgtcgaaa tgatgcctga tctctcttta ctgagtgaag ctaaggtgaa aggcatagag
     4741 ctcgagtcgt tgttagagaa actcaatagc cgcatcgagg ctctttacga tcgtgaacat
     4801 acgctggggc atgcgttctt tatgccggta aaaaatgcac tcgatgccgg tgatgaagaa
     4861 gctgcgttta aacaattgaa gatcgcattc cagaaaaaga tcattccgct tttacaggaa
     4921 tactttttcg atgactggaa caagatccgg ttggtgctgg cagacaatca aaagcaagac
     4981 gacaacctgc aattcgtgat tgagaaaacc gacgatctcg atacgctttt tggtaacaac
     5041 catggtttac gacgccatga tcagcaatca acagcttatg agctcaaaga tttcgatcaa
     5101 gagatctgga atattccaca ggcttatcgt tcaatttatc agccccaaca gactcccctt
     5161 gatgagcagg cagtaaatca tgggtgaagt ta
//
LOCUS       DAZFAH010000265         5116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFAH010000265 DAZFAH010000000
VERSION     DAZFAH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.267"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..496)
                     /gene="yehS"
                     /locus_tag="ACHJWB_004479"
     CDS             complement(26..496)
                     /gene="yehS"
                     /locus_tag="ACHJWB_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4315977.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(543..1262)
                     /gene="btsR"
                     /locus_tag="ACHJWB_004480"
                     /gene_synonym="yehT"
     CDS             complement(543..1262)
                     /gene="btsR"
                     /locus_tag="ACHJWB_004480"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4315978.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1259..2944)
                     /gene="btsS"
                     /locus_tag="ACHJWB_004481"
     CDS             complement(1259..2944)
                     /gene="btsS"
                     /locus_tag="ACHJWB_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4315979.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILNRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3166..3231
                     /locus_tag="ACHJWB_004482"
                     /pseudo
     CDS             3166..3231
                     /locus_tag="ACHJWB_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3560..4786
                     /locus_tag="ACHJWB_004483"
     CDS             3560..4786
                     /locus_tag="ACHJWB_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFO4315980.1"
                     /translation="MILIADMFAAKPELSQRCISGLLTTGPNDENVNNKPSSDMPNRI
                     KPVVSDSQIGIYTADSNTLKDGLCSQAHIGTQPYRPGADSPITVRSFLQITLSNNNYK
                     SLEHLQTRAENYLRYRKAEENILRSTVEGLTNPESPVFKQTAWMGHLERGLWKTETRW
                     DGNDREQLGKEALGSEEPKPGSPFYGSRGLKLSDSAHSAFSMMLCGSEGPFTKEQALS
                     GFELAQTGQVLAGRLKIQERVKFRADNRIDAQRNGTHSTRTPTGMDLSQDIGTIMRDK
                     AGLPVMSGTSGSSSDATLATRYAAEHFGKTWAAPGLSQAEGCKAISDLSHHYFRAEGS
                     SPPQSMATGINKVRYDAGMEEKYVNTLDIFTHSYPEIYAGVALTIAGAGGNDEQAMYN
                     VTQEAARILHEAETKD"
BASE COUNT         1415 a         1267 c         1165 g         1269 t
ORIGIN      
        1 gtttcgcatg gccgccatac ccgtcttagc ttttcttcac atgctggcgc gctgccagtc
       61 cacgcagaaa ataacgtaaa aattgatcgc cgcattcgcg gaagttttta tgatccggtg
      121 cgcgcatcat cgctgtaatt tccggcatcg aaacgcggaa ctgctgttcg gtgaggatag
      181 ccagaatgtc atcggttttc agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt
      241 tattaatgcg acgttccggc tccagtgccg gagcagactc atccttgccg cgtttttcat
      301 aaatcaggcc attgaggaat gacgacaaaa caatgtccgg acaacgctga aaaccctctt
      361 cgtcttcttt acgtagccag acggcgatct gttccgcggt ggcttcgaca ttacccagcg
      421 ccagaatacg caccaggtca ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa
      481 tatcgttact tagcatgagg ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc
      541 ttttacaggc caatcgcctc ttttaagctt ttcagataac ggcggctgac cggcacggtt
      601 aagccattac gcaaaatcaa ctcggcctgg ccgttatctt ccagacgaat ctcctgtaaa
      661 tgcgcgaggt taaccagata ctgacgatgg cagcgcagta gtggtgtacg actttccagg
      721 gtacgtaatg tcaattcggt aaagccctct ttcccttcgt ggctggtaac gtagacaccg
      781 ctcatccgac tgctgacaaa tgccacatct ttcatttgca gcaaataaat ccgactatgc
      841 cccgtacaag ggataaattt cagcgcctgt tgattttccg gtaacagcga aacatcctgc
      901 ttgctgcgct cctgacgcaa tcgcgccagc gttttctcca gtcgcgcttc atcaattggc
      961 ttcagcagat aatcaaaggc atgttcttca aaggctttaa tagcgtattc gtcaaacgca
     1021 gtgagaaaaa caatatacgg gcgatgttcc gggtcaagca tccccaccat ttccagacca
     1081 ctgatgcgcg gcatctggat atcgagaaac agcacatccg ggcgcagttt atgcaccgcg
     1141 ccgatccctt ccacggcgtt tgaacactct ccaacgattt caatatcgct ctgctcctgc
     1201 aaaaatacac gcaggttttc ccgtgctaac ggttcatcat cgacaattaa gactttaatc
     1261 atgcctcgtc cctccatggt agtcgtaacg ttattcgggt gtaactatca ggctcacagg
     1321 cgacgcttat tccatagtca tcgccaaacc gttcacgtaa acgcttatcc accagattca
     1381 tccccagccc actggcattg gttaccggtt gatacaaacc ggcattgtct tcgatctcca
     1441 gcatcaaatg ttgcccctca cgtcgggcgc tgattgccac tcgccctgta tccagcagtt
     1501 gtgatgtccc atgtttaatg gcgttttcca ctatcggttg cagggtaaac gcgggcaatt
     1561 gctgctggga taattcttgc ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg
     1621 ccttttcaat ttgcagataa gcgttcacat gttcaatttc gtcggcgaga gtaacaaact
     1681 ccgaaggccg ctttaagttt ttgcggaaaa aagtggaaag atactgcacc agctggctgg
     1741 cctgttcgct gtcgcggcgg atcaccgctt taatggtgtt aagcgcatta aacaaaaaat
     1801 gaggattcac ctgggcgtga agcagtttga tttctgactg ggtgagcatc gctttttgcc
     1861 gctcatattg cccggcaaga atctgcgccg aaagcagttg cgcaatcccc tcgcccagcg
     1921 tgcggtttat tgaactgaat aaacggtttt tggcttcata caatttgatg gtgcccatca
     1981 cgcgctgatt ttcgccacgc aacggaatta ccagcgtcga ccccagtttg cattgcggat
     2041 gcaaagagca acgataaggt acttcgttgc catcagcgta gaccacttca ccggtttcaa
     2101 tcgcttttaa ggtgtaagtc gaagaaatcg gtttgccggg taaatggtgg tcgtcaccaa
     2161 ttccggtaaa ggccagcaat ttctctcgat cggtaatcgc gactgcacca atatccagct
     2221 cctgatacag cacctgagcc actttcatgc tgttcacttc gttaaacccc tgtcgcaaaa
     2281 tgccttccgt cgaggctgcc actttcagcg cagtggcaga aaaagcagaa gtgtattttt
     2341 caaacatcgc gcgtttatcg agcaatatac gcataaacag cgccgcgccg acggtattgg
     2401 tgaccatcat tggtgcagca atattactca ccagacgcac cgcatcttca taaggtcggg
     2461 cgatcgcaag gatgatcagc atttgcacca tttcagcgac gaacgtgacg gcaccggcgg
     2521 taatggggtt aaagacttta tcagtgcgcc cgcgacggtt caggatgctg tgtaccaggc
     2581 cgccgagcaa tccttcaacg atggttgaga tcatgcaact tagcgcggtc atgcccccca
     2641 tcgaatatcg atgtaagccg ccggtcagac caaccagccc accgacgacc ggaccgccga
     2701 gtaagccgcc cattaccgcg cctatcgcac gggtattggc aatagaatcg tcaatgtgca
     2761 acccaaacca ggtgcccatg atgcagaaga tggaaaagac gatgtagcag agaaatttat
     2821 gcggcagacg aaccgtgacc tgcattaacg gtatgaataa tggcgtttta ctcattaacc
     2881 acgcaatgac taaaaaaacg cacatctgct gaagcagcag caataccaga ttaaaatcgt
     2941 acatacccgc aaaccacact tccctttaaa acgcgtaaca tacattgcct gcgtttaact
     3001 ttctttgaac tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg
     3061 caaaaataaa atcaccctat agatgcacaa aaaacgggca aaactacctg gttcgcaaaa
     3121 ctgcgtctaa agttaaaccg ggacctcgcg agcaagggtg agacgatggc gctttacaca
     3181 attggtgaag tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc gtgacgcaac
     3241 atttttaaaa atcaacgaag atcgtccagg agcgccgatt ttcaagggtt ggacagataa
     3301 ttcgttttcg atgtcgacga acttcgacgg atttcgccta taaaaagtgt cccatacatg
     3361 ccccattgat gccccataga caatatcagc aattgacact ggttatctat acaactaaaa
     3421 ataacacctg cacataacca cagttacagg agggtgttat gcgtgttgag agctgtatcg
     3481 ctaaagacaa aatcagcaaa atgaggtgta ctggcaatag cggacactac catttgttct
     3541 ttttttaagc agccatctga tgatattaat agcagatatg tttgcagcaa agcccgaatt
     3601 aagtcaaaga tgtatttctg gactcctcac aactgggcca aatgatgaaa acgtaaataa
     3661 taaaccgtca agtgatatgc cgaacagaat caaaccagtt gtaagcgatt cacaaatcgg
     3721 tatatataca gctgacagta atactctgaa ggatggattg tgtagccaag cccacatagg
     3781 aactcaacct tatcggccag gtgctgactc gccgataact gtgagatcat tcctacaaat
     3841 cacactttcc aacaataatt acaaatcatt agagcatctg caaacacgag cagagaatta
     3901 tctgaggtat cgcaaagcag aagagaatat tctgcgttcg accgttgagg ggctaaccaa
     3961 tccagaatct cctgtattca aacaaacagc atggatgggg catctggagc gtggcctttg
     4021 gaaaacggaa acacgctggg atggaaacga ccgcgagcag ctaggcaaag aggctcttgg
     4081 ctcagaggag ccaaaacctg gttcaccatt ttacggtagt cgtggactaa aattatccga
     4141 cagtgcgcat tctgccttct caatgatgct gtgcggttct gaaggtccat tcacaaaaga
     4201 acaagcactc tctggtttcg agcttgctca aactggccaa gtgctggctg gaaggctaaa
     4261 gattcaggag cgagttaaat tccgcgcaga caaccgcatt gatgctcagc gtaacggtac
     4321 acactcaacc cgcacaccaa caggcatgga tctttctcaa gatatcggca caattatgcg
     4381 tgataaagct gggctaccgg tcatgagcgg aacatccggt tcctcctctg atgccactct
     4441 tgcaacacgt tacgctgccg agcattttgg taagacatgg gccgctccag gcttaagcca
     4501 agcagaagga tgcaaagcta tctcggatct gtcacatcac tattttcgtg cagaaggctc
     4561 cagtccacca caatcaatgg caacgggaat aaataaggtt cgttatgatg caggaatgga
     4621 agagaaatat gtaaatacac tggatatatt tacacatagc tacccagaaa tttatgctgg
     4681 ggttgcattg acgattgctg gggctggcgg taatgatgag caggcaatgt ataatgttac
     4741 gcaagaagcc gctcgtattc tgcacgaagc tgaaactaaa gattgaacat gttctagtga
     4801 agaggcctac cccagaagaa tatattccac attcaaggca ttattgatga attttataat
     4861 caatgcgaaa aaactggcat tgattgtata cagattctcc tgaaggagaa tctgttctgt
     4921 cccttctgcc aagcagtgtt acgcaacgat caatctttcg cgttcctatt aatcaatgag
     4981 ttagagttga ttagtgacct gagacagagc attagcgcaa ggtgattttt tgtcctcttg
     5041 cgctaatttt ttgtcatcaa acatacagcc aatcagaagg gcataaagct gccaagcatt
     5101 atatgtctta gtttta
//
LOCUS       DAZFAH010000266         5114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFAH010000266 DAZFAH010000000
VERSION     DAZFAH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.268"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1562)
                     /locus_tag="ACHJWB_004484"
     CDS             complement(<1..1562)
                     /locus_tag="ACHJWB_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4315981.1"
                     /translation="MAFKHYDVVRAVSPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWG
                     VDKPLYKDLIGRTKAALKKNPKNVLLAVVWMQGEFDFGGTPANHAAQFGALVDKFRAD
                     LADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTD
                     ENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLAT
                     AILRHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSAS
                     GGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGAL
                     AANQYVMAFYWPV"
     gene            complement(2063..3121)
                     /locus_tag="ACHJWB_004485"
     CDS             complement(2063..3121)
                     /locus_tag="ACHJWB_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4315982.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(3273..3470)
                     /locus_tag="ACHJWB_004486"
     CDS             complement(3273..3470)
                     /locus_tag="ACHJWB_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4315983.1"
                     /translation="MLKQRDMTETARAVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(3683..4372)
                     /locus_tag="ACHJWB_004487"
     CDS             complement(3683..4372)
                     /locus_tag="ACHJWB_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4315984.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFATSSWRRTLLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWNEFKTQSGTRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(4369..4728)
                     /locus_tag="ACHJWB_004488"
     CDS             complement(4369..4728)
                     /locus_tag="ACHJWB_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4315985.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLEIKITEVGCA"
     gene            complement(4741..>5114)
                     /locus_tag="ACHJWB_004489"
     CDS             complement(4741..>5114)
                     /locus_tag="ACHJWB_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4315986.1"
                     /translation="HTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRW
                     VKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKH
                     GSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1155 a         1504 c         1176 g         1279 t
ORIGIN      
        1 accggccagt aaaacgccat aacatactgg ttcgcagcca gcgctcccga aaccttaaag
       61 cggcagcgaa tctgcccccc tttctgtaac agagccgcac cgttgcccgc ggcgtactcc
      121 agcacccagc tgcttttacc tgcttccttg gtcagcttca ctgccttacc tccggttccc
      181 tccgcatcgc tgaccacttc tgccctgccg ccactggctg accatccctg tactttcagg
      241 cttccctctg actcgctggc aaggtaagag agcagtgttg tgacgcctgt ggcttctgca
      301 ccggaagacg atgacgggcg cacctctgat actgtcgatg atgcccccgc gtttagcgcc
      361 actcttcccg catggcgcaa aatcgccgtt gccagacggt cggaaataat cccgcggcga
      421 gcccatgaac tgaaatggct cgccctgtcc tgtgacgtcc aggtggctga gctgtcacgc
      481 catttcgaac cgtaataccc gatacccgga atgtccgggt cttcttccgg tttgttcgtc
      541 ggcacattca ccccgttctc atcggtcatg aacggtacga aatggatatt cttttccgtt
      601 ttgtttttgt agctgccgta caccgtctgg tacgtggatt cgttcttctg cttccagaaa
      661 tacgtcgtat ctccacatat ccagggaaca ccgccagcag agccaccgac gcactgacct
      721 gccatatccg ccaggtctgc acggaattta tcaaccagcg caccaaactg tgctgcgtga
      781 tttgccggcg taccgccaaa atcaaattcc ccctgcatcc acaccacggc aagcagcaca
      841 tttttcgggt tcttcttcag tgctgctttt gttcgaccga taaggtcctt atacagcggc
      901 ttgtccacac cccagcgggt tgaattctcc gaagcaccac tcgcgtcact gtatgtgcca
      961 tcagctccgg tggtgaacgc tgaaccacca cgacagcacg gaaccagcag aatgcccgca
     1021 ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc cacggtaccg
     1081 tactgcccct ttgacaggtc cgctttcgga tggttaaggc ggctcatgtc ctgcacatca
     1141 tgcagacaat ggtccgccgg aatgatgtcg ttatatttac aggcgacacc gcccggtgtc
     1201 accgtactgc gacgcgccag ctgcttaatg cgcgggtccg gacggtcata tgtctccggc
     1261 agcggaaggc cttcaccata tgccatgctg tttgactgcc ccgccagaac cacaacaaag
     1321 taatactccg ggtctctggt ggcgctgatt actgtgcctt ctccatccga cggcttcacc
     1381 acaacaggtg tgctcacatc accttctgcg acaatcgcct gaataagtgc tgcgccatca
     1441 tccgtatacg aagaaaacgg cccgccgtat ggttgccatc cttcacgaat tttttgagca
     1501 agtgcatcag caaggtctga aggcgacacc gccctgacca catcgtagtg tttaaatgcc
     1561 atgaatcctc ccggccggga taatattgtg agtaaaatga ggagcgggct gaaatccgga
     1621 agttacagga caatggcaga agagagacaa cagcccgcaa tacgaaaaag gccgcgctat
     1681 tgcgcagagt gattactgtc ggatattatt cgccagctga aatattactt cacgttttgt
     1741 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgtccat aagaaagccc
     1801 ctccggagag gggctggaga gtggcgctat gtgccattgc atggtgccgg gtgcctcccg
     1861 gtgaattcag tatcagcacc tgaatccgca cagaaaggat agagtagaaa cacctgcgct
     1921 gatatgcccc tccgctcagg gggattcacc atgccagttt cttttaacaa actccccgca
     1981 aaccagacaa ctgtcaaccg tctgaattgt gagacattta aaattttcgg ggcgtacctg
     2041 atacccggtt aattctgcaa tatcatccgt tctgactgac taaatcctgt acttccctga
     2101 ccgtctgctc aaaacgttca gtctccagct caacgccagt tgcacgacgc cccagcgcca
     2161 tcgcggcttt gactgtcgaa cccgacccca tgaagaaatc tgcaaccagg tcacccggac
     2221 gactgctcgc actgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc
     2281 cgggatagta ctgcaccggt ttatgcgtcc acacatccgt gtacggcacc tgcgccgtca
     2341 cgccaaaata ccgccgcaga tgcttatatt cactctgcag ttccgcatac tgccggttca
     2401 gtgaagtata cgtctccagc agctggtggt ggggcttttc cagttcaccg cgctgatgct
     2461 tctcttctgc cacccgggca aacagcgcct gtaatttcag ataatcgctt tcgttcggta
     2521 gctgccactg actggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatcca
     2581 ctatctgttt tgccgttatc cccagggcag cacgcgcatc acgaaagtaa gaaatcagcg
     2641 gagccatcac atgctgtttc agtgccctgc ccttcgcctc atacccggca tctttcggac
     2701 gatacggccc ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc
     2761 tcaggctttc cttgttgcac ccgttccagc gtccggacgg cttcgcccag ataatatggt
     2821 tcagcacact gaagcgttca cgcatcatga tttcaatgtc agatgccagg cgatggccac
     2881 agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt
     2941 ccagccattt caggtaatcc tcatcacccg tccactggtt atcccagccc tcaggcttca
     3001 ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga
     3061 taaattccag gcagtcggcg ttgatcaact cacaactgga tatttttaca gtattaaaca
     3121 tggatcatta agcctgtctc tgataggctc ataccgcttt tgcgcaaagc agatgggcct
     3181 gaggtttgct tgtgacccca acgcatgagc agatggctgg tgagtgcccc taacacccac
     3241 cagccgccca tttaccacaa ataaaaaagc cttcaggact gaaggcgtct gtaacaacca
     3301 aactgatagt ctgccagacc cgccataaca agctgggtca gtattagctg gcagcgttcg
     3361 cgtgaaaggt acgtattctg tgcaatctcc ccgactgtcg ccggttcggt ggcacttaat
     3421 tcattaaaaa ctgctctggc ggtttctgtc atatcccgct gtttcagcat gtctttttcc
     3481 ctttttcggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
     3541 ccggtattca ccgccgccag cgcgtttact gtactgacgc gattttcgga cataaaaaaa
     3601 ccgcctggcg gcagtttttt tcttattttg tcatcgcgta caaaataggc aaaatatcag
     3661 atttatacga aacatacgcg atttaattga cttttgcaat atctcgtcgt gaaaaggtcg
     3721 ctttttgttg cgctcttatt ttcacggagc aaatcaagga ttctctatcg aggcgcttaa
     3781 aaatatcgca catctcacgc cagtagttcg cataattatg gctccagtta tcaggcttaa
     3841 ctccacacag tctggcaagc tcctgtctct ggtagacctc acacccggta acccatcctc
     3901 tgacatcctg tgccgccagc cagatcaact tcttcacacg ctccagcgtt ttcactgcaa
     3961 tttttctggt accagactga gttttaaatt cattccacac ccactgcgtt atcgcgatct
     4021 gatgctccca gcaaatgttt ccgccataac accacaacaa ccacgccttc tgatgttctt
     4081 ccagttccag aagggtacgc cgccacgatg atgttgcaaa ctcaacagga ctgaccaggg
     4141 caattgatga gcctttcgcc agtgattgtt tacccgggat tgggggatta tcccgcgtga
     4201 tcatttttcc ggttacctca tcgcggtaac gaattttttt gcgtctgtaa cgccctgtat
     4261 cgaacagggc attttcctgc caggcttcca gttgaccttt tgtcgcccca ctgagatccg
     4321 cagtggcaat catgagttgc tcacgaacaa actgtaaata ctggttattc atgcacaccc
     4381 cacctctgta atttttatct ccagccgtcc accagatact ggcttgccac gtacaatatt
     4441 gatttcatca aactgctcat cgtccattaa caaccccgcg tgcgtcagcg catccagcgg
     4501 tgctttcaga atattgtcca ggtcacggcg gcgcttatcc ggtggctctg caataatttt
     4561 tattgccagc cgtccggaca ggcttaattt cagtcgctgc tggcgaacaa taagcgccac
     4621 tgcccggcga taacgctccc cggcttttga tacaaaatat gtgctgccac ggcgtcgcca
     4681 gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca gtcacctctt
     4741 ttacccaagc acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg
     4801 agaaccatgc ttttcttcga acgccagcgg atccgcatga agctcgttgt gatgctcccg
     4861 acacagcggt agcgtgaaaa tatcgtgaga ttttgtcccc attccgccct gaccatgacc
     4921 aatcaggtga tggggatcgt cggctggctt accacaacac gcacacggct gtgtctttac
     4981 ccagcgtgtg tatttctcat ttacccagcg gcgacgttta ggtcgtttca tgaaggattc
     5041 cggagactcc ggatcaacgg caatgctgac caccgtcttt tcctgtggtg ggttctgttg
     5101 ctggtgggtg tgag
//
LOCUS       DAZFAH010000267         5084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFAH010000267 DAZFAH010000000
VERSION     DAZFAH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.269"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            14..97
                     /gene="yqiM"
                     /locus_tag="ACHJWB_004490"
     CDS             14..97
                     /gene="yqiM"
                     /locus_tag="ACHJWB_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4315987.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            119..781
                     /gene="yqjA"
                     /locus_tag="ACHJWB_004491"
     CDS             119..781
                     /gene="yqjA"
                     /locus_tag="ACHJWB_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4315988.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            785..1168
                     /gene="mzrA"
                     /locus_tag="ACHJWB_004492"
     CDS             785..1168
                     /gene="mzrA"
                     /locus_tag="ACHJWB_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4315989.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            1315..1683
                     /gene="yqjC"
                     /locus_tag="ACHJWB_004493"
     CDS             1315..1683
                     /gene="yqjC"
                     /locus_tag="ACHJWB_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4315990.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            1721..2026
                     /gene="yqjD"
                     /locus_tag="ACHJWB_004494"
     CDS             1721..2026
                     /gene="yqjD"
                     /locus_tag="ACHJWB_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4315991.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2029..2433
                     /gene="yqjE"
                     /locus_tag="ACHJWB_004495"
     CDS             2029..2433
                     /gene="yqjE"
                     /locus_tag="ACHJWB_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4315992.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            2423..2722
                     /gene="yqjK"
                     /locus_tag="ACHJWB_004496"
     CDS             2423..2722
                     /gene="yqjK"
                     /locus_tag="ACHJWB_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4315993.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTPLKQQQLRG"
     gene            2908..3300
                     /gene="yqjF"
                     /locus_tag="ACHJWB_004497"
     CDS             2908..3300
                     /gene="yqjF"
                     /locus_tag="ACHJWB_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4315994.1"
                     /translation="MKKLEDIGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            3370..4356
                     /gene="yqjG"
                     /locus_tag="ACHJWB_004498"
     CDS             3370..4356
                     /gene="yqjG"
                     /locus_tag="ACHJWB_004498"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4315995.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            4649..5014
                     /gene="yhaH"
                     /locus_tag="ACHJWB_004499"
     CDS             4649..5014
                     /gene="yhaH"
                     /locus_tag="ACHJWB_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4315996.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1241 a         1249 c         1299 g         1295 t
ORIGIN      
        1 aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca gcaataacat
       61 tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat
      121 ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa
      181 tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc ttgaaaacgg
      241 cttgcttcca gcggcctttt taccgggcga cagtttactg gtattggtcg gcgtgttgat
      301 tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg ccgccagcct
      361 cggctgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa
      421 ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca
      481 cggtttatcg gcgctgttaa ttggtcgctt tattgcgttt gtcagaacac tgctgccgac
      541 gattgccggg ttatcagggc tgaataacgc gcgctttcag tttttcaact ggatgagcgg
      601 tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa cgccggtatt
      661 tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt
      721 gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta
      781 agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca ctgttttact
      841 tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc
      901 gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct ggcatcacct
      961 tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac
     1021 atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca
     1081 tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt
     1141 acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat cactcacttt
     1201 ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt
     1261 gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa
     1321 taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc
     1381 ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa
     1441 aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca
     1501 gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg
     1561 gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgataa gattgccaaa
     1621 cgcgaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     1681 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     1741 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     1801 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     1861 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     1921 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggcattgg
     1981 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     2041 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     2101 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     2161 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     2221 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     2281 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     2341 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     2401 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     2461 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     2521 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     2581 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     2641 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgcccctcaa
     2701 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     2761 aattttttga agatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     2821 attctctcca tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc
     2881 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatattgg tgtactggta
     2941 gcgcgcattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     3001 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg
     3061 attctgcttg agttcggtgg tggtctggca atcctgttcg gtttcctgac tcgcaccaca
     3121 gccctgttta ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct
     3181 gaaggcgtca actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg
     3241 ctggcaatta ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa
     3301 gcacgcgcta tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag
     3361 gagagaaaaa tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa
     3421 tctaccggcg gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat
     3481 ggcgcacctg gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc
     3541 tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga
     3601 ctggaaccgt ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc
     3661 tttgatgaca gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat
     3721 cagctttatc tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg
     3781 gacaaaaaga accacaccat cgtcagcaac gaatcagcag aaatcatccg catgttcaat
     3841 accgcgtttg atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg
     3901 aaaattgacg aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct
     3961 ggttttgcca ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg
     4021 gcgcgactgg agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa
     4081 gccgatattc gcctgtggac cacgctggtc cgttttgatc cagtgtatgt gacccacttc
     4141 aagtgtgata agcaccgcat cagcgattac cttaatctgt atggcttcct gcgtgatatc
     4201 taccagatgc cgggaattgc cgaaacagtc aatttcgatc acatccgtaa tcattacttc
     4261 cgcagccata agaccatcaa ccctacgggg attatttcaa ttggtccatg gcaggatctc
     4321 gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct
     4381 ctgataatta gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac
     4441 ctacaaaaca tttatattca atatgttgca atgaccgcgc aggccagata agcgtagcgc
     4501 atctggcgat tttgcgcttg tcatcagtct tagcgcgcct tcacagcact ttattaattc
     4561 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     4621 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta
     4681 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     4741 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg
     4801 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcagttca
     4861 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgttgt tcttaatccc
     4921 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     4981 ccgctttgga ccagatccga aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga
     5041 ctcatccggc ttttatcatc atgttgatga atgaataatt tact
//
LOCUS       DAZFAH010000268         5023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFAH010000268 DAZFAH010000000
VERSION     DAZFAH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.270"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..442
                     /locus_tag="ACHJWB_004500"
     CDS             <1..442
                     /locus_tag="ACHJWB_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315997.1"
                     /translation="FFSEYIVPLSGSGITNKYTDYDIKYGIDDGETIALHVIIKNLMT
                     VNKNTDFNDKNTIHHNNSKTLISYSSNIYTDKYLKYISEDTIKATSEKLKNIYCASGS
                     FHKLSPQERLFYEARKQRKSIILHYYADSGESLIFNIGVSPESC"
     gene            complement(454..3468)
                     /locus_tag="ACHJWB_004501"
     CDS             complement(454..3468)
                     /locus_tag="ACHJWB_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4315998.1"
                     /translation="MPVYAQAEDLNNGLIPSGNVLPEQTGFDVALPEGANPEPLPPEP
                     SVWGAAMRQNNILSGFFRPARQFEPVEGYNPYADKNELHGYEYWGAKFADSRSPEETA
                     WIKQQIDDENEDRRLLSDAGVVGTLASIAAGMDPVTVASMFIPGAQGGALARIGSQIA
                     IGAAGTALSEVVLNNQQITRSWGESAAHVAAGAMMSGVFASAGVALSPSVRAAATREV
                     ADALDNMSITSATDRAAASLSDGGSVGAMKIDTAALDDLTPVSGGWVGKAAWKAGSYL
                     TPLTRLMESPSKTVRKTALELAENNFTLKGNERGIETPVAVETRTRGWQREEAAVVVG
                     NKQAYAKYKADGGDMSFDSFRQQVGNAMRSGDVHANPVVQETAQAMRTVLNRVKVEMQ
                     KLGLLPPDEELKALGQASYFPRIYKVGKIISERDKFRRILVDWWSRGNKTLDPEDAEI
                     AADIVINKITGAKVPQDFVSVFSVKAAGSTKERTLNVSDSLIRDYLESDVNYVLQRHI
                     REAAAEIELTRTFGKRTMTERLQLIEDEYDSLLREVPEKIKAKYDESVANLKARYESN
                     GEVVPQGKLDSLMRKYEKELRKEQSRLSKSRANDLRDITALRDRLVGTYGMPDDPSSF
                     FVRAGAFLRDVNFTTKLGGMTVSAIPDLARGVMVNGFRNTMKGYASQISQSPAFKASK
                     EEMLKMGIGLEAVLHSRSRAIGDLVDSSSRTTAVEAGMERITDAFGKLTLMDRFNDIN
                     KSMNGMLTSDGILSGAFSARRMAKLGINDNMAARIRSEFEKHGEVIDGWHIGNFDKWD
                     DQYVAGVFQSAVLKDVNNTIITPGIGDTPLWASTPMGRTIFQFKSFTTASYNRALLGG
                     LQEGTAQFYYGTVFQIALGSLVYALKEASKGKNVDWSPEKLVLEGIDRSGILGPLMEY
                     NNMAEKATGGAVGLGALFGTGTQSRYASRGFVGSLFGPSFGLADSIIDVTAGVLNGDA
                     GDRIVHNVRTLIPGNNLFWIAPLINQVDPVMR"
     gene            complement(3468..>5023)
                     /locus_tag="ACHJWB_004502"
     CDS             complement(3468..>5023)
                     /locus_tag="ACHJWB_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFO4315999.1"
                     /translation="EKQRKLILEQLEPAINNTMAQLYNGEVPDYIPAQETIIRGYGKN
                     ADKIINQLDIAIDNARIFQAIQYLPPSQQQEEMQKVKPEVNDPHYALKLDAYGKLSAL
                     LQRSNEAIQAQRDSRRFNEALTIGEKLDPSNKSMQKAADYTEMAQNFRINDASTHDGV
                     VRLVAQTGIMPSQIITQLSAVSRSSNQEVVKNAAELFSRLYETDNASIGNMPKDMQGF
                     YLTVKQLTDSGMSADAAIEQAQNLTYNQTDALKAQLASTQSSKEYKNDRSKAMNSAVS
                     NMAQWFRWDPSADDQTPEAARFRNDYQTLYDINYRVAGGNADVAKQMTNQQIARTWSI
                     SEVNGEAQFMKYAPEALYQYGPSGWQAAQWKAEKERLMYGERKEIITTSPTLLGITSG
                     NAPVVETKTPESRINGELFITPDVLTPRNGDYAIMVRTKDENGIDRVQPFYNKHGRPM
                     RWEPSLEDWEPYKKMQQEREQYEQEEIMRGQAIRNFKDKHRALDEQYQRLHNERMDKF
                     KDYFSWGSK"
BASE COUNT         1326 a         1297 c          968 g         1432 t
ORIGIN      
        1 atttttctca gagtacatag tgccgctatc aggaagcggt ataacaaata aatatactga
       61 ctatgacatc aaatatggaa ttgatgatgg ggagactatt gcattacatg taatcattaa
      121 aaacctaatg acagtaaata aaaatacaga cttcaacgat aaaaatacaa tacatcacaa
      181 caacagcaaa acattaattt cttatagtag caacatctat acggataaat acctaaaata
      241 catctcagaa gatacaataa aagcaaccag cgaaaagtta aaaaacatat attgtgcatc
      301 cggcagtttt cacaaattat cgccgcaaga gcgtttattc tacgaagcaa gaaaacaacg
      361 taagtcaata atattacatt attatgctga ttcaggagag tcactcatat ttaatattgg
      421 ggtttcccct gaatcatgct aaaggcgttt aactcaccgc atcaccggat ccacctggtt
      481 tattagtggc gcaatccaga acaggttatt gcctggtatc agggttcgga cattatgcac
      541 aatacgatca ccggcatcac cattcaacac tcctgcggtc acatcaatga tgctatccgc
      601 aagaccaaat gacggtccga atagagatcc tacgaatcca cgactggcat acctagactg
      661 tgtgccagtg ccaaataaag cccccagccc aacagcacca ccagtagcct tttcagccat
      721 gttgttatat tccatcaatg gcccaagaat accggatcta tctataccct caagcaccag
      781 cttctctggt gaccagtcaa catttttccc tttcgatgct tcttttagcg catagaccag
      841 tgagccaaga gcaatctgaa atacagtgcc ataataaaat tgcgcagttc cttcctgtaa
      901 cccaccaagt agcgcacggt tgtatgaagc cgttgtgaat gatttaaact gaaatatcgt
      961 tcgccccatt ggagtactcg cccataaagg tgtgtcacca ataccggggg tgatgatagt
     1021 gttattaacg tctttcagaa ccgctgactg gaatactccg gcaacgtact gatcgtccca
     1081 tttatcaaag ttaccaatgt gccatccatc aattacctca ccatgtttct cgaactcact
     1141 gcgaatacgc gcagccatat tgtcgttgat accgagtttt gccatgcgac gtgcagaaaa
     1201 cgcaccagac aaaataccgt ctgacgtgag cattccgttc atggatttgt ttatgtcatt
     1261 aaatcgatcc atgagtgtca gcttgccgaa ggcatcagta attcgctcca ttcctgcttc
     1321 gactgctgtt gtcctggaag aactgtcaac aagatcacca attgcacgag aacgtgaatg
     1381 tagtacagct tccaatccaa tccccatctt caacatctct tctttgctgg ccttaaatgc
     1441 cggtgattgg gatatctgag aagcatagcc tttcatggtg ttacggaaac cattaaccat
     1501 aacccctctg gccagatctg gaatagctga tactgtcatt ccaccgagtt tggtcgtgaa
     1561 gttcacatcc cgcagaaaag cgccagcacg aacaaaaaac gaagacggat catcaggcat
     1621 accatatgta ccaacaagac gatcgcgtaa tgctgttatg tctctgagat catttgctct
     1681 tgattttgaa agtctggact gttctttccg taattccttt tcgtactttc gcattaatga
     1741 atcgagttta ccctgaggaa caacttcacc attgctctca taacgtgctt tcagatttgc
     1801 cacactttcg tcatatttcg cctttatttt ttcaggcact tcccgtaaca gactgtcata
     1861 ttcgtcctca attaattgca gacgctcagt catagttcgt ttgccaaatg ttctcgtcaa
     1921 ctcaatttct gctgccgctt cacggatatg acgttgcagc acgtaattca catcactttc
     1981 aagataatcc ctgataagac tatcagaaac atttaatgtt ctttctttcg tactacctgc
     2041 ggcttttaca gaaaatacgc tgacaaaatc ctgtggaacc ttagcaccag taattttatt
     2101 aattacgata tccgctgcaa tttcagcatc ctcaggatcc agtgttttat ttcctctcga
     2161 ccaccagtca accaaaatac gtcgaaattt atcgcgttca ctgattattt ttccaacttt
     2221 atatatgcgt gggaaatagc ttgcctggcc taatgctttc agttcttcat ctggcggcaa
     2281 taaaccaagc ttttgcattt caactttcac ccgatttaat acagttcgca tcgcctgcgc
     2341 cgtttcctga acaacaggat tagcatgcac atcaccgctt cgcatagcat tcccaacctg
     2401 ctgacgaaat gaatcaaaac tcatgtcacc accatcagct ttatactttg cgtatgcctg
     2461 tttatttccg acaacaacag cagcttcttc acgctgccat ccacgtgtac gggtttctac
     2521 agctaccggt gtttcaatcc ccctttcatt tcctttaagg gtgaaattat tttcggctaa
     2581 ctccagcgct gtttttcgca ccgtcttgga cggagactcc attaaccttg tcaaaggagt
     2641 aagatagctc cctgctttcc atgcagcctt tccaacccac ccaccggaaa caggggttaa
     2701 atcatccaga gccgctgtat caattttcat agcaccaaca ctaccaccat cggaaagcga
     2761 agcggcagcc ctgtcagtcg ctgatgtaat gctcatatta tcaagagcat cagcaacctc
     2821 acgtgtggct gcagcccgga cggatggcga aagcgcaaca ccagcactgg caaacacgcc
     2881 gctcatcatc gcacccgctg caacgtgagc ggcactttca ccccatgagc gtgttatttg
     2941 ctgattattc agtacaacct cgcttaatgc tgtaccggca gcaccaatcg caatctgtga
     3001 gccaatacgc gccagtgccc ctccttgagc accgggaata aacattgacg caacagtaac
     3061 cggatccatt cccgcagcaa tactggcaag ggttccaact acgccagcat cagacaataa
     3121 acgtctgtct tcattttcat catctatctg ctgcttaatc cacgccgttt cctctggcga
     3181 tcgggaatct gcaaatttcg ccccccagta ttcataaccg tgcaactcat ttttatcagc
     3241 atatgggtta taaccctcga ccggttcaaa ctgtctggct gggcggaaaa aaccagacag
     3301 aatattgttc tgtcgcattg cagcccccca tacggaaggc tcaggtggca atggctcagg
     3361 attagcccct tccggaaggg caacatcaaa cccagtttgt tccggcaaaa cgttacctga
     3421 cgggatcagt ccgttattaa gatcttcagc ttgtgcataa actggcatta tttagatccc
     3481 cacgaaaagt aatctttaaa tttgtccata cgttcgttat gcaggcgctg atactgctca
     3541 tccagagcgc gatgcttgtc tttgaagttt cgtatagcct gtccacgcat aatttcttcc
     3601 tgctcgtact gctcccgttc ctgctgcatt ttcttatagg gttcccaatc ttctagtgat
     3661 ggttcccaac gcataggacg cccatgtttg ttataaaacg gctggacccg atcgatgcca
     3721 ttttcatcct tagttcttac cataatggcg taatcaccat tacggggtgt taacacgtca
     3781 ggggttatga ataattctcc attaatacga ctctcagggg tttttgtctc aactacagga
     3841 gcattacctg acgtgatccc aagcaacgtc gggctggttg ttataatctc tttgcgctca
     3901 ccgtacatca gccgttcttt ttcagctttc cactgcgccg cctgccagcc tgacggccca
     3961 tattgataaa gcgcctccgg tgcatatttc ataaactgcg cttctccgtt aacctcgctg
     4021 atactccagg tgcgggctat ctgctggttg gtcatttgct tcgctacgtc agcgttacca
     4081 ccagcaacgc ggtagttaat gtcatacagc gtctgatagt cattacggaa tctagctgct
     4141 tccggcgtct ggtcatccgc agacggatcc caacggaacc actgcgccat attgctgaca
     4201 gcagaattca tcgccttgct gcgatcattt ttgtactctt ttgaactctg cgttgatgcc
     4261 aattgagctt taagagcatc ggtctggttg tacgtaaggt tctgcgcctg ctcgatagcc
     4321 gcatcagcag acatgccaga atcagttagt tgcttaacag tcagataaaa accctgcata
     4381 tccttcggca tatttccaat agatgcattg tctgtttcat ataaccgact aaacagttcc
     4441 gccgcatttt taaccacttc ctgattgctg gatcgggata ctgctgaaag ctgcgtgatg
     4501 atctgcgaag gcattatgcc agtctgagcc acaagccgaa caaccccatc atgagtggag
     4561 gcatcattaa tacgaaagtt ctgcgccatt tctgtgtaat cagcagcttt ctgcattgac
     4621 ttgttgcttg ggtctaattt ttcacctatt gtcagcgcct cattgaatct gcgtgaatcc
     4681 cgttgcgcct gaattgcttc atttgatctc tgaagcaatg cagacaattt tccgtaagca
     4741 tcgagtttta acgcatagtg aggatcgtta acctcaggct tcactttctg catttcttct
     4801 tgctgctgag aaggaggcaa atactgaatt gcctggaata ttctcgcgtt atcaatcgct
     4861 atatccagtt gattgattat tttatctgcg ttttttccat accccctgat gatggtctcc
     4921 tgagccggta tataatctgg aacctcaccg ttatatagct gggccatggt gttattaata
     4981 gctggctcaa gctgttctaa tattaacttc ctttgctttt cta
//
LOCUS       DAZFAH010000269         5016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFAH010000269 DAZFAH010000000
VERSION     DAZFAH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.271"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..665)
                     /locus_tag="ACHJWB_004503"
     CDS             complement(<1..665)
                     /locus_tag="ACHJWB_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4316000.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTALDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENIT"
     gene            complement(750..947)
                     /locus_tag="ACHJWB_004504"
     CDS             complement(750..947)
                     /locus_tag="ACHJWB_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4316001.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            complement(959..1447)
                     /locus_tag="ACHJWB_004505"
     CDS             complement(959..1447)
                     /locus_tag="ACHJWB_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4316002.1"
                     /translation="MNLSLTLEADSLNVQALNMGRIVVDVDGIELAELINNVTENGYS
                     VRVVEDSGQQSTCTPPPLTILAGIRCSTAHITETDNAWLYSLSHQSSNFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(1444..1821)
                     /locus_tag="ACHJWB_004506"
     CDS             complement(1444..1821)
                     /locus_tag="ACHJWB_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4316003.1"
                     /translation="MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRSHYRTVNDITLGKRTEAKR"
     gene            complement(1868..2242)
                     /locus_tag="ACHJWB_004507"
     CDS             complement(1868..2242)
                     /locus_tag="ACHJWB_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4316004.1"
                     /translation="MSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(2317..2538)
                     /locus_tag="ACHJWB_004508"
     CDS             complement(2317..2538)
                     /locus_tag="ACHJWB_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4316005.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            complement(2607..3083)
                     /gene="radC"
                     /locus_tag="ACHJWB_004509"
     CDS             complement(2607..3083)
                     /gene="radC"
                     /locus_tag="ACHJWB_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4316006.1"
                     /translation="MQQLSFLPGEMTPGERNLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGRQVFSFAEHGLL"
     gene            complement(3099..3584)
                     /locus_tag="ACHJWB_004510"
     CDS             complement(3099..3584)
                     /locus_tag="ACHJWB_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4316007.1"
                     /translation="MTTSSHNSTTPSVSVTTASGNNQSQLVATLIPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDEKWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(3676..4494)
                     /locus_tag="ACHJWB_004511"
     CDS             complement(3676..4494)
                     /locus_tag="ACHJWB_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4316008.1"
                     /translation="MRLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     SYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEITSQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4598..4786
                     /locus_tag="ACHJWB_004512"
     CDS             4598..4786
                     /locus_tag="ACHJWB_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316009.1"
                     /translation="MSEKPSTGEPRIKLFSGISFRVPAASASLTFRVADNPTEMHSRV
                     ILDKHIAVSGYDSNRLPV"
     gene            complement(4742..4870)
                     /locus_tag="ACHJWB_004513"
                     /pseudo
     CDS             complement(4742..4870)
                     /locus_tag="ACHJWB_004513"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4905..>5016)
                     /locus_tag="ACHJWB_004514"
     CDS             complement(4905..>5016)
                     /locus_tag="ACHJWB_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316010.1"
                     /translation="KATEESNGHRVSLPLSVFDPLDYHPLLITVSGKNVN"
BASE COUNT         1138 a         1278 c         1377 g         1223 t
ORIGIN      
        1 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
       61 aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac
      121 ctcactaggt tgttgacgat aattttactg ccaaatttac aggggcaatt ggttttgtaa
      181 ttccagctca gccctctgaa gaggttgatc acacctcgct caaatacttc gtccttattc
      241 tggtgcagtt gttcaaacgt actcaggata ttcgcctcgc tgatttccgg aaaattatcg
      301 tactccagtg atcggtacca tgtatcacgt gcctgtgcat ccattaatga cagcatccct
      361 gaacgttgca tcaggtcacg ccagatttcg cggtcaaggt tgcgggtgat agctttcatt
      421 gcggtttccg ttttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag
      481 tccagtgcag ttttaccgcc aatgctttcc gtcataactg agatttcagc cagttgccgg
      541 ataagtgtgt cgatttgcag cagtgtactc tttcgtacag ccagaatgtg gctgactgag
      601 gttgagcaaa taacgtccgt atgcccgttg accacttccg gacgggaggc tgtgatatca
      661 gacataatcg atattccttt ttacagataa acgaaaacgc cagccgtatt caggctggcg
      721 tcagtggaat gaagagtggt gaatgagact caccggttct ggtctggaag ctgcagctgg
      781 cgcagggtac ggatatcctc cctggcctgt tcaatacagg gcaacagtgc tgccgaatcc
      841 gttttatctt catcgttgtc aaagataatt cccgattcgc agtcgatatt gtcctgcagc
      901 catgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt gtttctggtt
      961 accagtcgaa agtgggtaaa tccggcagac attcggcgct ggcatccaga tgaatgagac
     1021 tgacgccata acgtcgggta agtgtgacaa ccagacggcg gaacgttctg gacaggccca
     1081 ggcgtttaag ccgcagaacc gggtatgacc acgcatcagt acgtaacaga tatccgctac
     1141 ctgtaaaatg aatccattct gattcgccga agttactgct ctgatgcgac agcgagtaca
     1201 gccaggcgtt gtctgtttcc gtgatatgtg cggtgctgca gcgtatgccg gcaaggattg
     1261 ttaacggtgg tggtgtgcag gttgactgtt gcccggagtc ctccaccacg cggactgaat
     1321 aaccgttttc agtgacattg ttaatcagtt cagcgagctc aataccatcg acgtcaacga
     1381 caatgcgtcc catgttcagt gcctgtacgt taaggctgtc ggcttcgagc gtcagggaaa
     1441 ggttcatcgt tttgcctccg tgcgtttacc cagggtaatg tcatttaccg ttctgtagtg
     1501 gctgcgggtc atcaggccgg ttgcccggcg ggcccggaga atatcaatgc tgttgattaa
     1561 ctgcgacggg gctcctgcgc tgaatcccgg ctggtcagtt cgtaccagcg cgtatttttc
     1621 aacgagaaag ttcaccgcat cacacagtga aatccctgcc tcaatatgct gctcaatcac
     1681 acgttcatca gcgaacggtg tgtcattcag cgtgaggcca tagtgctggt ccagcagtcg
     1741 ggtgagcagt gtctgccaga tggtgacggg agacgggcag cccgatgcct cccgtacgtg
     1801 tgtgtcaggt aatgttttca tcttaaattc tctgtaactg gtagtgctaa aggtgagata
     1861 ttcggggtta tgaggtggtt gcgggtgcca gtgtcggata aacagccata taaacgtaac
     1921 cacagctgcc gagggtgtcg gcttcgcagg tcagccctct ggcatacagc gtgacggtat
     1981 gctgatggcg gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca
     2041 gcggaaaggc ctggtccaga tgatacgcat ccgcgtcgct aaactgcccc ctgataccgg
     2101 cgcggtcggc aaggtaatgt aattgattgc cctcctgcac cagacgtgcc ccgaaacagg
     2161 gcgtcacggt gcaggggagc ccccaccagg ggcggtcgtt gttgtcgttg ggataatttg
     2221 tctcatggag tgtgcctgac acgataaaat cctcacaaaa aatcagttaa gaatggtccg
     2281 gtggcagcga taattctgct catcatttct cctgactcag ttcagtgtga cgctcatcag
     2341 ccggacatac gagccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg
     2401 aatatcctgt acttcgcgac cggtatacgc ctcagcgcta ccgtgccagc gatacttacc
     2461 ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc
     2521 acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag cgaaaacagg
     2581 tatgatgtgg tggtgacggt gacgggttaa agcagaccat gttcggcaaa ggagaaaacc
     2641 tgtctgccac caactatcag atggtccggt acccggatat ccaccagggc cagtgcctgt
     2701 accagacgct ccgtaataag ccggtctgcc ttactgggtg tgacttcacc ggacgggtga
     2761 ttgtgtgcca gcaccacggc agcggcattg tgatacaggg cgcgtttaat cacttcccgg
     2821 ggatggactt ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga
     2881 ttctggttgt tcagatacag cacccggaat tcttcacgct ccagtcccgc catgttcaga
     2941 atcagccatt cccgtgccgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg
     3001 cggtccaggg ttttcagggc ccgctgaatg aggttgcgct cgccgggcgt catctctccg
     3061 ggcagaaagg aaagctgctg cattgtcccg gcctccattc agtcgatgat gcgcataatg
     3121 gcgctgcatt ccggatgctg cagggcgtaa tcccgcaacc ggtaataatg gaccgtcatg
     3181 gcataacatt ccgtacggca ggcatgatgg ctgtacgtca tcagacaggc agcaatgccg
     3241 gcggcttccg ggctcatttc agcgcggtta ccgttcatgg cattgaacag tacccatttt
     3301 tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac
     3361 agattccaga taccaccgca gtagtcttcg caaagacggt ccatccagcc gaagacacgg
     3421 ggctccaggg tcacccactg tggaatgagg ccaaaatgtt gcggccagaa gcttatgcgc
     3481 tgttcatcag ggatgagggt ggcaaccagt tgagactggt tattccctga tgcagttgtt
     3541 acggaaacag aaggtgtggt ggaattatgc gaagaggttg tcatgagatt attccttata
     3601 aaaagcaaat gaatggaaga atgcccgggg actggtacag acgtgagtca gggctgcact
     3661 ttcagtaaaa cggcatcagc gcatactctc cagcagcgtt tctgccatca cccacaatgc
     3721 gcgattgagc ttaatgtccg tgtcgatgct gtgaatggca cgggtgtgga tacgttttcc
     3781 ttttgcactg cgaccggaaa tcccgccttt cagcatattc tcctggatgg tctgataagc
     3841 actccacagg tccttaccgt aatcctcccg gcgtcgtggc gtcagaatgt cggcggtggt
     3901 gacgggctga tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg
     3961 tgccggtggc ggcagaatca gcgactgcat ggcatcacgc ttttcctcaa tccggtcaaa
     4021 aacccccacc acctcgtaag ccccttcaat aactttctcc accacatttc cccggtgtgg
     4081 aacacgcaat tctcccagag actgaccgca gaccagtcca ttggtacaaa tggcacgaaa
     4141 atatccgggt aacatctggt agctggaggt accgtcatgg gagttgagca gaataatttc
     4201 aggcacatgc tgactggtta tctctccggc ccgccgcaga cgcagcatgt gtttggtata
     4261 ttcccggcgg ctctggtcgc gcacacgggt ctggcaggcg aagaacggct ggaagccttc
     4321 ctgctgcagg ttttccagaa cggtgatggt gggaatgtat gaataccgtt cactgcggga
     4381 ggtgtgccgg tcttcaccaa aaatactcgg aacgtggtac atcagttctt cgcgtgtcag
     4441 cgggcgctcc cggcggatct gatttacatg accaaaacgg ctggctaatc gcatactttg
     4501 ctccttatcg gtggttaaga tttactggtg taataaatga aaaaagccgc gtctcccgga
     4561 gaagacgcgg cctgatggat gaaatcaatg aggtttattg tctgagaagc cctccactgg
     4621 cgagccacgc attaagctgt tttccggcat cagcttcaga gttccggcag cgtcagcgtc
     4681 gctgaccttc cgcgttgcgg ataacccgac ggaaatgcat tcccgggtca tcctggataa
     4741 acacattgcg gtaagcggtt atgacagcaa ccgcctgccc gtatgagaaa gatccttcag
     4801 ctaggacata ctctgtgtgt aacccggcat atctggtttc tcctgataaa tagcctctgc
     4861 cacaacgtat ggcagaggct gaagcatgaa actgacttca gggatcagtt aacatttttt
     4921 ccggaaacgg taatcagcag tggatggtag tccaggggat cgaaaaccga taacggcaga
     4981 ctgacacgat ggccgttact ttcttcagtt gcttta
//
LOCUS       DAZFAH010000270         4931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFAH010000270 DAZFAH010000000
VERSION     DAZFAH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.272"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            159..425
                     /locus_tag="ACHJWB_004515"
     CDS             159..425
                     /locus_tag="ACHJWB_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316011.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            422..1021
                     /locus_tag="ACHJWB_004516"
     CDS             422..1021
                     /locus_tag="ACHJWB_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4316012.1"
                     /translation="MMMPVLQKLPFSGLPFSGICGYSFSAAAKSAAGRGNPSMLSATY
                     DAPCVFFCVAINATERQIMVWCVVSRANGTPCILPASAHYTAESMVAQAGQPPGWPVS
                     DNAGISTPVWAIAIERGNSGDSVIYAVIGGCLMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1014..1301
                     /locus_tag="ACHJWB_004517"
     CDS             1014..1301
                     /locus_tag="ACHJWB_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO4316013.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1294..1749
                     /locus_tag="ACHJWB_004518"
     CDS             1294..1749
                     /locus_tag="ACHJWB_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316014.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            1885..2205
                     /locus_tag="ACHJWB_004519"
     CDS             1885..2205
                     /locus_tag="ACHJWB_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO4316015.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2220..4553
                     /locus_tag="ACHJWB_004520"
     CDS             2220..4553
                     /locus_tag="ACHJWB_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4316016.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETEAARKNAAALAQTLMAKTRPGTGNAYLTRKDFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLASDRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT         1080 a         1438 c         1372 g         1041 t
ORIGIN      
        1 agctatctgg cgctgatgta caaaaaatgc acagccattg taaggctgcc ggaacaaatc
       61 ccccctgttg cgtctgccga aaatattcac aaaataaagc gctacccgaa gccggacgga
      121 cttatccggt gctgtatgga cattaacgag gtagcccgat gcaagctgtt ttttcttccc
      181 cgtctcccgc ccctgtgacg ccactgatgc cgctgccgga catcacgcag gagcgttttt
      241 tacgtctgcc ggaagtgatg cacctgtgcg gcctgtcacg ctcgaccatc tatgaactca
      301 tccgtaaggg ggaatttccg ccgcaggtga gtcttggcgg taaaaatgtg gcctggctgc
      361 actctgaagt caccgcatgg atggccgggc gcattgccgg acgcaaacgg gggtacgacg
      421 catgatgatg cctgttctgc aaaaacttcc tttttctggc ttgccttttt ccggcatttg
      481 cggatatagt ttttccgctg ccgcaaaatc ggcagccggg cgtggaaacc cgagtatgtt
      541 atcggcgaca tatgacgcgc catgcgtctt tttttgtgtc gcaatcaacg ccacagagcg
      601 ccagattatg gtgtggtgtg tggtcagtcg agcaaatggc actccgtgca ttctgcccgc
      661 gtccgctcat tataccgcag agtcaatggt agctcaggcg gggcagcctc cgggctggcc
      721 ggtttccgat aacgccggta tttccacccc cgtctgggct atcgccatcg agcgtggaaa
      781 ctccggcgat agcgtcattt acgctgttat cggaggttgc cttatggcta cgacccttac
      841 cccctcacac cctgaatttg tctttgtgtt tgcggctgtc cgtcgcgcag accgtcatcc
      901 ccgtatctgt atgcttcgca ccgtcgccgg tgatgaacgc agcgcccgcc gttcccttgt
      961 ccgtgactat gtgctctccc ttgctgcccg tctgccggtg gtggaggtgt cccgtgcgta
     1021 ataaaaaagc ccctcagacc gtctcagcgc gtcatgacgc ccgtgaacac ctcagcattg
     1081 aggcttacca taagctcaac cgcgccagcg ccgtatccca gtttgttggg ggtgatttga
     1141 ttcaccgtga actctccggc ctgcatcagc tctacattcc gcatattttc agttacctga
     1201 atgaagatat tgattttgtg ctgaatgaac tgaaagccaa aggcctgtgc cgcgattttc
     1261 tcgcccagca gaaagaccgg ggagacagga cgcatgtttg attttcccca gcccggtgag
     1321 atttaccgtt ctgccggttt tcccgatgtg gccgtggtcg gcattctgga agacggtatt
     1381 ccgtgggaaa tgccgtaccg ctgcccggaa attgtctgga acccgtaccg ccgtaaattc
     1441 agtatccttg tgcgtatcct cgccgacggg cgcaccacag acatcccgct ggggcgtttt
     1501 ctgcgggaat ttacctgtga ccgtcctgac ctgttcaaac gcagccccgt aaaccggcat
     1561 gcggtactga aagaaatggc cggagacccg gaattacaga aatggcggga gaaatatctg
     1621 gatatttacc cgcaggaccc tgttccggtc agccgggcgg caccggtggc gcgggaatgg
     1681 cgggaaattc cccgcacgga gcctgacccg gaaatcaccc cggataacag ttaccgcaat
     1741 tatctgtaat taaaaaacga cacccgaaaa attaaatgtg cgtattcgcg cagggatacg
     1801 cacgtcttca ggagacgcag atatgcctta tcagttaatg caaccggcac agaatgcagt
     1861 catctgtcac agggaggaaa gcaaatgaaa acacccttac cgcccgtctt acgcgctgcc
     1921 ctttaccgtc gcgctgtcgc ctgtgcctgg ctgaccgtgt gcgaacgtca gcaccgctac
     1981 ccgcatctca cccttgagtc actggaggcg gccatcgccg ctgagctgga aggcttttat
     2041 ctgcgccagc acggtgagga aaaagggcgt cagatagcct gtgccctgct ggaagattta
     2101 atggaatccg gccccctgaa ggccgcgccg tcgctgtcct ttctcgggct ggttgtgatg
     2161 gatgaactct gtgcccgtca cataaaagcg ccggtactgc actgaaggag aactacacca
     2221 tgaaaatgaa cgtaaccgcc accgtcagtc atgcgctcgg ccactggccg cgtattctcc
     2281 cggcgctggg gattcaggtg ctgaaaaacc gtcatcagcc ctgtccggtc tgtggcggga
     2341 gtgaccgctt ccgttttgat gacagggagg ggcgcggcac ctggtactgc aatcagtgtg
     2401 gtgccggtga cggcctgaaa ctggttgaaa aggtgtttgg tgtttccccg tccgacgcgg
     2461 ccgcaaaggt ggctgccgtg accggcagtc tgcccccggc tgacccggca gtgacggccg
     2521 ccgccgttgc tgaaacagaa gccgcccgga agaacgccgc cgcactggca caaaccctga
     2581 tggcaaaaac ccgtcccgga accggtaacg cctacctgac ccgcaaggac tttcccggcc
     2641 gggaatgccg gatgctgacc ggcacacaca gagccggtgg cgtgagctgg cgcgccggtg
     2701 accttgtggt gccactgtat gacgacagcg gcgaactggt taaccttcag ttaatcagtg
     2761 ctgacggccg taagcgcacc ctgaaaggcg gacaggtcag gggcacctgt cacacccttg
     2821 aaggacagaa tcaggccgga aaacgtctgt ggatagcgga gggatacgcg accgcactta
     2881 ccgtacatca cctgaccggt gaaacggtga tggtggcgct ttcttccgtg aacctccttt
     2941 ctctggccag ccttgcccgg cagaagcatc cggcctgtca gattgtcctt gcctcagacc
     3001 gtgacctcag cggtgacggc cagaaaaaag ccgccgcagc cgcagatgcg tgtgaaggtg
     3061 ttgttgccct gccgccggtc ttcggtgact ggaatgatgc cttcacgcag tacggcgggg
     3121 aagccacccg taaggccatt tacgatgcca tccggccacc ggctgaaagc ccgttcgaca
     3181 ccatgagcga agcggagttt tccgccatga gtaccagcga aaaggccatg cgtatctatg
     3241 agcattacgg cgaggcgctc gcggttgatg ccaacggcca gcttctgtcc cgttatgaaa
     3301 atggtgtctg gaaggtgctg ccgccacagg actttgcccg ggatgtggcc ggactgtttc
     3361 agcgtctgcg cgcgccgttc tcctccggga aggtggcctc cgtggtggac accctgaagc
     3421 tgattattcc gcagcaggaa gccccctccc gccgcctgat tggctttcgt aacggcgtgc
     3481 tcgacacgca gaacggtacg ttccacccgc acagtccgtc acactggatg cgtaccctgt
     3541 gtgatgtgga tttcaccccg ccggtggacg gtgaaacgct ggaaacccac gctcccgcgt
     3601 tctggcgctg gcttgaccgt gctgccggtg gtcgtgcgga aaaacgcgac gtgattctgg
     3661 ccgcactgtt tatggtgctg gcaaaccgct acgactggca gctctttctg gaggtgaccg
     3721 gtcccggcgg cagcggcaaa agtatcatgg ccgaaatagc caccctgctg gccggggagg
     3781 ataacgccac gtcggccacc attgagacgc tggaatcccc gcgtgaacgt gccgcgttaa
     3841 ctggcttctc actgatacgc ctgccggacc aggaaaaatg gagcggcgac ggagccggac
     3901 tcaaggccat caccggcggc gatgcggtgt ccgttgaccc gaaataccgg gatgcgtact
     3961 ccacgcacat cccggcggta attctggccg tgaacaataa cccgatgcgc ttcaccgacc
     4021 gcagcggcgg cgtgtcacgc cggcgggtga ttattcactt cccggaacag atagccccgc
     4081 aggagcgcga cccgcagctt aaggacaaaa tcacccgcga gctggcggtc atcgtgcgtc
     4141 acctgatgca gaagttcagc gacccgatgc tcgcccggtc actgcttcag tcccagcaga
     4201 actcagacga ggcgctgaac atcaaacggg atgccgaccc gacgtttgat tttatcggct
     4261 atctggaaac cctgccgcag accagcggca tgtatatggg gaacgccagt atcatcccgc
     4321 gtaattaccg taaatacctc tatcacgcct atctggccta catggaggca aacggctacc
     4381 ggaatgtact cagtctgaaa atgttcgggc tggggctgcc ggtgatgctg aaggaatacg
     4441 gactgaatta cgagaagcgc cataccaaac aggggataca gaccaacctg acactgaaag
     4501 aggaaagcta cggcgactgg ctgccaaaat gtgacgaccc tgcaacaacc tgacccacct
     4561 gaccggcatc tgccggtctt ttttttatcc cgacctcccc cgaaggtgaa caatccactg
     4621 ttcacccttc accgtatatt cacccgttat cacactgaaa ttaaaagaga aaaatgaaag
     4681 gtgaacagtg tgaacaatca aatcaaaaaa aacttttttc tccctgtgcg attttcagta
     4741 cgggggatta atcaccggta tgagtcacac cggcagaatg ccggaggtga agaatcgaat
     4801 gttcaccctt cacccattat tcaccaccta tcatgctgaa ataaaaggag aaaacagaaa
     4861 tgtgaacagt gtgaacagtt ctttcgaaaa aaaacttttt tcttattttt aattgtgcga
     4921 aaaacaagcg a
//
LOCUS       DAZFAH010000271         4890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFAH010000271 DAZFAH010000000
VERSION     DAZFAH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.273"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            188..1162
                     /gene="hemB"
                     /locus_tag="ACHJWB_004521"
     CDS             188..1162
                     /gene="hemB"
                     /locus_tag="ACHJWB_004521"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4316017.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1268..2119)
                     /gene="tauD"
                     /locus_tag="ACHJWB_004522"
     CDS             complement(1268..2119)
                     /gene="tauD"
                     /locus_tag="ACHJWB_004522"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4316018.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRAHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2116..2943)
                     /gene="tauC"
                     /locus_tag="ACHJWB_004523"
     CDS             complement(2116..2943)
                     /gene="tauC"
                     /locus_tag="ACHJWB_004523"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4316019.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2940..3707)
                     /gene="tauB"
                     /locus_tag="ACHJWB_004524"
     CDS             complement(2940..3707)
                     /gene="tauB"
                     /locus_tag="ACHJWB_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4316020.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3720..4682)
                     /gene="tauA"
                     /locus_tag="ACHJWB_004525"
     CDS             complement(3720..4682)
                     /gene="tauA"
                     /locus_tag="ACHJWB_004525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4316021.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLVVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1137 a         1406 c         1242 g         1105 t
ORIGIN      
        1 accaagagct ataaacaaag attaaaaata ttttcagtga atatgaaatt attgctattt
       61 ctattacaaa gatgtcctgg tctctctttc attctcttct tgtcaacatc acgacaactt
      121 tcgtaaaaca ctcctgcccc gcttcaggta tactgtgcac cttaattcca caaacatcag
      181 gcagaccatg acagacttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg
      241 cgctatgttt gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga
      301 agaagaaatt gacgactaca aagccgttga agccatgcca ggcgtgatgc gcattccaga
      361 gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc ggtattcgtt ccgtgatgac
      421 tttcggcatc tctcaccata ccgatgaaac cggcagcgac gcctggcggg aagatggact
      481 ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg tcatgtcaga
      541 cacttgtttc tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg agcatggcgt
      601 cgacaacgac gcgactctgg aaaatttagg caagcaagcc gtggttgcag ctgctgcagg
      661 cgcagacttc atcgcccctt ccgccgcgat ggacggccag gtacaggcga ttcgtcaggc
      721 gctggacgct gcaggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc
      781 ttccttttat ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag
      841 ctatcagatg aacccaatga accgtcgcga ggcgattcgt gaatcactgc tggatgaagc
      901 ccagggcgca gactgcctga tggttaaacc tgctggagcg tacctcgaca tcgtgcgtga
      961 gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat
     1021 gattaagttc gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt
     1081 aggttcgatt aagcgtgcgg gtgcggatct gattttcagc tactttgcgc tggatttggc
     1141 tgagaagaag attctgcgtt aatttttttc ctctcagaaa gggggagtta acctgttgaa
     1201 agtacggtga acgccccctc accctaaccc tctccccaag ggggcgaggg aaccgtccgc
     1261 actcgtatca ccccgcccga taaaacggtt tatccccaag aatcgtcgcc cgatgcataa
     1321 tccgccgctg tggcaggtaa tcggcattgg catagtgctg agtcacgcgg ttatcccaaa
     1381 tcgcaatatc attcggttgc cagcgccaac gcacctgaaa ctccggttta gtgatatggg
     1441 cgaataaaaa gcctaacaat gcttcgctct ctttctcgct gacatcgaca attcgcgtag
     1501 taaagccttc attcacaaac agcgcctgtt taccgctcac cggatgcgct cgcaccaccg
     1561 gatgtagcaa cggaggattt ttcgcgaccg cctcgcgcca gcgttgatgt tcctcctcgg
     1621 ttttgcggta tttgtattcc gggaacgatt tacggaaatc atgctccgca cgcagcccgc
     1681 tcagcagctg gcggaaggga acagagagcg cctcataggc cgcaataccg ctggtccaga
     1741 gcgtatcacc gccggtcgaa ggtaattctt tagctgccag aatcgcccct gcgggtggcg
     1801 tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc tggcggatta tcgttatggg
     1861 tatccagcac gatgatctcg tcaacccctt cggcatgagg gtaaacaggg tgaatatgca
     1921 gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg cgtaatggct tgatcgcgca
     1981 gaaacaccac ctgatggcgc agcaccgcat ggtaaagctg ttcaaactga ttatcgctta
     2041 acgggcgcgt caggtcggca cccgaaattt gtgcgccgat atacggcccc agcggggtaa
     2101 tgctcagacg ttcactcatt gtacttctcc atgccagggc gtcagacggc gctgtaacgc
     2161 gcgcaaaccc agttctaaaa gaaaggcgat aatcgcaatc accgcgattc ccgccagcac
     2221 cacgtcagtc gcgagaaatt cacccgctga ctgaaccata aatcctaaac cgcgcgtcgc
     2281 ggcaatcagc tccgccgcta ccagcgtaga ccagcccaca cccagaccaa tgcgtaatcc
     2341 ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac cacagcacct gcgcacggct
     2401 ggcacccagc gactgggcgg cacgaatgcg aacctgctgc gcgcttttca cccccgccag
     2461 cgccgacatc gccaccggtg caaaaatcgc taaatagatc agtaagatct tcgaggtttc
     2521 accaatacca aaccagatca ccatcagcgg caaataagcc agcggcggca ccggacgata
     2581 aagttcgatt accggatcca gaatgccgcg caccgtcggg cttagcccca tggcgatccc
     2641 gaccggaata ccgatcacca ccgccgcgag tagcgccagc acaatgcgcg tcaggctggc
     2701 tgccagatgc tgccacagcg tggcgtccat aaatccctgc ggtccggcaa tggtgagcag
     2761 ttttgccagt acttgttgcg gcggcggcaa aaacagcggg ctaatcagtt gcagcgccgc
     2821 caccgtccac catacagtga gtaaaaccgc taacgtgcca atgcttaagg tcacctgacg
     2881 cgagagcggc cagcgccatt tcagccgccg cgaatgcagt ttttcattaa tgagcacact
     2941 catgagaacg cctcccgttg ctcaaatacg cggcttaaaa catattcgcg catggcgatg
     3001 aattgtggat cggacttgat gctgcggctc gactctcccg caacaaagcg gcgagcaaag
     3061 ttgagcggca gccgctccag cacacggcca gggccggatg aaagcagaac cagttcagtc
     3121 gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt gcccgtctcc
     3181 tgccagagtt tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc cagcgcacca
     3241 aacggttcgt cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc aatccccacc
     3301 cgctgacgtt gaccaccgga aagctgccag atatagcgtt tttctgcgcc ttccagcccc
     3361 acttttttca gcatctggtg cgcgatttcc agtcgctgca ttttctctat acctgccaat
     3421 tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg gtagtagccc ttcattctga
     3481 aaaaccaccc cgcgctctgc tcccggtccc tcaatacgct tacccgccag ttgaatgctg
     3541 ccatgctgat aaggcacaaa accggcaatc agattcagca acgtggtttt accgcagccg
     3601 gacggcccca gcaccaccag tagctcgccg ctttccagcg tcaggttgat atcttccagt
     3661 gccggtttgc cgccataatc ggcgtaaaga tgagagattt gcagcatctg cgcctccttt
     3721 tattgcacga agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc cgggaccttg
     3781 ccctgctctt tcaaaaactg cgcggtgtcg atgatcgctt tgttcaccgg tccggtcagt
     3841 tctaccgttt gttgctgcgg cgtcagatag gtattcccct tcaccagccc cggaacgtca
     3901 ccttccggca cgccgcttaa acgcgccagt ttgctgatgt tttccggctg tttcagccac
     3961 gcgtctgggt tagcaatgta cggttgctga gcatcgatgg cgcttttagc gaacgctttc
     4021 acgacctcag gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc cagcgttggc
     4081 gcaccccact gcccgacctg ttcagaatcg gtcaacacct tgccgtcttt ttccagggcg
     4141 ttaaccgccg gtgcccagac ataagcacca tcaatatcac cccgctgcca tgcggcgata
     4201 atcgcgggcg gctgcaggtt cacaatctcc acttgccctg gtttaatccc ccagtgtttc
     4261 agcgccgcca gcaggctgta gtgggtggtg gagataaacg gtacggcaat gcgtttgcca
     4321 atcaaatctt cgggtttgct gatagttttc tttaccacca gcgcttcgga gttacccagt
     4381 tttgacgcca gcaagaagac ttcaatcggc acctgttggc tggctgcaac cactaatggg
     4441 ctggaaccga ggttgccgat ttgcacgtcg ccagaagcca gcgcccgcac gatgctggct
     4501 ccgctgtcaa acttacgcca gtccacggtt gctccgcttt ctttagcaaa ggtgttgtcg
     4561 gcctgagcca ctttcgccgg ttcggctgag gtttgatacg ccacggtgac gttcaccgcc
     4621 tgtgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg tgttacgcga tgaaattgcc
     4681 atgattgtct gctcccctgt cttgttatgt gagcagtatt caggaataaa aacattcatt
     4741 aaaagaatta gtcgttatcg tacagatgat tttattctta gcaaaaaaac ggcaaaggaa
     4801 agattaaata tgcaattact ccgttctaac aattgggaaa attactacac ttcattttag
     4861 tgatgcataa acaaccacat tacgagattt
//
LOCUS       DAZFAH010000272         4858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFAH010000272 DAZFAH010000000
VERSION     DAZFAH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.274"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..1994
                     /gene="mngA"
                     /locus_tag="ACHJWB_004526"
     CDS             78..1994
                     /gene="mngA"
                     /locus_tag="ACHJWB_004526"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO4316022.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2012..4645
                     /gene="mngB"
                     /locus_tag="ACHJWB_004527"
     CDS             2012..4645
                     /gene="mngB"
                     /locus_tag="ACHJWB_004527"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO4316023.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4670..>4858
                     /locus_tag="ACHJWB_004528"
     CDS             4670..>4858
                     /locus_tag="ACHJWB_004528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316024.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIF"
BASE COUNT         1184 a         1136 c         1345 g         1193 t
ORIGIN      
        1 gtgaaattga tcacataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
       61 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgtgtctga
      121 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
      181 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
      241 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
      301 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
      361 cggaagcggt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactacgc
      421 atatgcaact gctgacagcg ctgaccacgc gcctggcgga tgatgagatt cgggcgcgta
      481 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
      541 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaacggcc tgtccggcgg
      601 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
      661 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
      721 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
      781 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
      841 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
      901 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
      961 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
     1021 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
     1081 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
     1141 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
     1201 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
     1261 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
     1321 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
     1381 aacctgtcgc ctggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
     1441 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
     1501 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
     1561 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
     1621 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
     1681 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
     1741 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
     1801 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
     1861 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
     1921 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
     1981 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
     2041 cccgcatatg cattgggatc gggagtggta tttcaccacc gaagagtcac gcattctgct
     2101 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
     2161 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
     2221 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
     2281 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
     2341 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
     2401 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
     2461 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
     2521 tagcgaagtg acggcgcagg tgctgccgtt gggttatgcc atcggtaagt acttacctgc
     2581 cgacgaaaac ggattacgca aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
     2641 tgtaaccaaa gagattttat tgccgaatgg tcatgaccag atgccattgc agcaaaatat
     2701 cttcgaagtg atggagaaac ttcgcgaaat ttatcctcaa cgaaagtttg tgatgagccg
     2761 ctttgaagag gtattcgagc agattgaagc gcagcgtgaa agtctggcga ccctgaaagg
     2821 tgaatttatt gatggcaaat atatgcgcgt gcatcgcacc atcggttcca cgcgcatgga
     2881 tatcaaagtt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
     2941 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
     3001 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
     3061 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
     3121 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
     3181 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
     3241 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
     3301 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
     3361 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
     3421 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
     3481 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga acgctacaac tggtagataa
     3541 agatagcggt gtgcgctatg accgggtgtt gcaaattgaa gaaagctctg atgatggtga
     3601 tgaatatgac tattcacccg caaaagaaga gtgggtaatg acttctgcaa cagcgaaacc
     3661 gcaatgcgag attactcatg aggcctggca gagcagggct gttatccgct atgacatggc
     3721 ggtgccgctc aatttgttgg aacgaagcgt ccggcaatcc acgggcagag taggggtgga
     3781 gatggttgtc actctgagtc ataacagcag acgtattgat gtggatatca accttgataa
     3841 ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca tcttttaaca ccgatagtgt
     3901 tctggcagat acccagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
     3961 ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgt
     4021 tgccttacag gaagggcgta acggcatggc tgtctttagc gaagggttac gtgaatttga
     4081 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg tgggcttact
     4141 gggcaaagaa gatctgcttt taaggcctgg gcggccttcg ggaattaaaa tgccagtccc
     4201 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
     4261 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
     4321 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
     4381 ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta agaaagctga
     4441 agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa cctgtgatgc
     4501 gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg atgaacacat
     4561 taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg gccagtcacg
     4621 gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata tgcgcggcca
     4681 gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt tattcggtct
     4741 acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca agcgtagcgc
     4801 atcaggtaat tttgcgttta tcttcactct caagccacgt atatgtggct ttattttt
//
LOCUS       DAZFAH010000273         4854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFAH010000273 DAZFAH010000000
VERSION     DAZFAH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.275"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..800)
                     /gene="kduD"
                     /locus_tag="ACHJWB_004529"
     CDS             complement(39..800)
                     /gene="kduD"
                     /locus_tag="ACHJWB_004529"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4316025.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(830..1666)
                     /gene="kduI"
                     /locus_tag="ACHJWB_004530"
     CDS             complement(830..1666)
                     /gene="kduI"
                     /locus_tag="ACHJWB_004530"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4316026.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1953..3134)
                     /gene="yqeF"
                     /locus_tag="ACHJWB_004531"
     CDS             complement(1953..3134)
                     /gene="yqeF"
                     /locus_tag="ACHJWB_004531"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4316027.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3389..4618
                     /gene="yqeG"
                     /locus_tag="ACHJWB_004532"
     CDS             3389..4618
                     /gene="yqeG"
                     /locus_tag="ACHJWB_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4316028.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1259 a         1237 c         1066 g         1292 t
ORIGIN      
        1 ctgccatgcg gcagggtcat aaaagtaaga agaatgaatt aacgcgccag ccaaccgcca
       61 tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag gaacactacc
      121 ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc gaggatttcc
      181 gcgctacgtt gttcatctgc ccgtagttgt tgagtattgt tggtcgccat gtaacccggg
      241 gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa tcgcgtcaca
      301 cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc ctggaaggag
      361 agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa gtgtttcgcc
      421 gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc gtcccagtct
      481 ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt caccaggata
      541 tcaatatgac caaactccgc taccgcgcga tccagcagtg ctggaatacc atcaatcttt
      601 cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg cttgatggtt
      661 tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc cagccccaac
      721 gccatcccct ggcccagccc agtatcacag ccagtgacga ccgcaacttt accttcgaga
      781 gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac tagcgcaaat
      841 ctttaacggc cacatggtcc atatcatcaa agacctggtt ttcaccgacc atgccccaga
      901 taaaggtata agctttggtt ccgacgccgg aatgaatcga ccagctcggg gagatcaccg
      961 cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc atgtggaaaa
     1021 cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc tcgtgagtgt
     1081 gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc atactcaatt
     1141 ggcaggtttc cagcacatcc gggacaaaat atttgttaat tgtgcgacgg ttactggtga
     1201 ggttatcgcc taacgtgact ggagatactt cgtccggagt gacttttttg gtgggatacg
     1261 tcgtatgcgc gggtgcgcaa ttgtaataaa actttgccgg agtggcggta tcaatactgg
     1321 caaagacaac ttcttttgca cctttaccca catacagggc gtcgcggtga ccgatttcat
     1381 agcattggcc atcgacagta atcgtaccgg caccgccaat attgataaca cctaactcgc
     1441 gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca acggaaaccg
     1501 ttttagttat cggcataatg ccgccaacaa taattcggtc aatgtggctg taaaccatgg
     1561 tgtactcatc ggcgacaaat acctcttcaa ccaaaaattc attgcgcagc ccttgggtat
     1621 ccagcgtttt tgcgtgcgca ctgtggatgc tctgtcttac gtccacatca acctccgata
     1681 atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt ctctataaag
     1741 ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt tcatttttaa
     1801 gttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat ggaaaaatga
     1861 gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga ttaatgacgg
     1921 cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat ggtcaatgcc
     1981 acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt acgtttcacc
     2041 atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg gtgaccgagt
     2101 gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat cttgccaacc
     2161 gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc agccaactgc
     2221 cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc catcaatgcc
     2281 ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa attcaacgct
     2341 cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat ggatgatgca
     2401 ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc taaaccttct
     2461 gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg accgttactt
     2521 tgggtcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc cgctcgcgct
     2581 ttttgttgcg agctaagtgc gtaagcatcc tgcaattgac ggctgatgcc atattcgcga
     2641 gccagatttt cggcggtgac accaatatga taatcattga aggcatccca taacccatca
     2701 tgcacaagac tgtcaaccag ctggctattg ccaagctgtg caccggtgcg gctatcagtc
     2761 agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat atcagcctcg
     2821 ccacactgta tcgcctgagt agccagatgc agtgctttaa gtccggaacc gcagacgtca
     2881 ttaatcgtga ttgcagaaac gctattaggc agaccacctt taatagccga ttgccttgcc
     2941 ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc caccgcatat
     3001 gcaggaacgc cggtacgttc tattaacgct ttcacgacca gactaccaag ttccacggcg
     3061 gaatgacccg ctaacgcacc gcgaaagcag ccgataggtg tccgtaacgc cccgacaatc
     3121 acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat gattgttaat
     3181 tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat atgcctgagc
     3241 agtagtcaga gtcataactg gcacgtaagg tttgcaacca ctaacccacc aatagagggg
     3301 tagatagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc ctccattttg
     3361 tttaatttgt agatgatacg ttcagataat gtctaatatt tggtctaaag aagaaactct
     3421 gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc ttcctattca
     3481 gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct ggcctttaac
     3541 atattggcca cataaagcct tatgccagtt catcctctca tcgaaaacat cagcaggtga
     3601 agggataacg ggcgcagtaa cacactacta tggcaagaag attggtaatc tgattaccac
     3661 gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa ttaccaactc
     3721 acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta tgttggtgag
     3781 tctgggtgtt gtattaatcc tgaatctcat ttttctgatg ggacgccatg caaccattcg
     3841 ggtaatgggt tttttggtat tcccattgat tgcctatttc ttatttcttt ccatttacct
     3901 tgtcggtagt tggcaacctg atctattaac gacccaggta gagttcaatc agaataccct
     3961 tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata cgcccattat
     4021 ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg ataaatgcaa
     4081 aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct ttgtctttag
     4141 ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag gggtcaccat
     4201 tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ctgtcaattt ccgggattat
     4261 tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg ttattgaagg
     4321 tgccacggag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa gtcgtgcatt
     4381 taaccgcgca ctatcaatta tgttggtatc gctgatcacc ttcattgttt gttgcattaa
     4441 cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca tgatactttt
     4501 catcatgcct acgttgtcaa cgtatctgat cccggcgctt aaaccctggc gttccatcgg
     4561 aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct ttagctgata
     4621 tccattgtga atggttcaaa attgctacaa ttttgaacca ttccagtttt cataatgttc
     4681 attcgttatt attccgcact atcacttccc tcatcatatc ctagtattat cagttagtta
     4741 aacacacaaa gagatctgaa catcttaaat aagactattt aagatgcata acttagattc
     4801 gcaagatatc ttctggtcat tcaaaaacaa tttgcgatat agatattctc atac
//
LOCUS       DAZFAH010000274         4777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFAH010000274 DAZFAH010000000
VERSION     DAZFAH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.276"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHJWB_004533"
     CDS             complement(<1..596)
                     /locus_tag="ACHJWB_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4316029.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHJWB_004534"
     tRNA            complement(711..786)
                     /locus_tag="ACHJWB_004534"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHJWB_004535"
     tRNA            complement(793..867)
                     /locus_tag="ACHJWB_004535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHJWB_004536"
     tRNA            complement(984..1068)
                     /locus_tag="ACHJWB_004536"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHJWB_004537"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHJWB_004537"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHJWB_004538"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHJWB_004538"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4316030.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHJWB_004539"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHJWB_004539"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4316031.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHJWB_004540"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHJWB_004540"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4316032.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1209 a         1214 c         1088 g         1266 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcctgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggaag agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg ctttccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgcaaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgtttg
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagct
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAZFAH010000275         4751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFAH010000275 DAZFAH010000000
VERSION     DAZFAH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.277"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..3073)
                     /locus_tag="ACHJWB_004541"
                     /pseudo
     CDS             complement(1..3073)
                     /locus_tag="ACHJWB_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(3134..3706)
                     /locus_tag="ACHJWB_004542"
     CDS             complement(3134..3706)
                     /locus_tag="ACHJWB_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316033.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRISGRDVSTSGLTAQ
                     LHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(3703..4446)
                     /locus_tag="ACHJWB_004543"
     CDS             complement(3703..4446)
                     /locus_tag="ACHJWB_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4316034.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            complement(4452..>4751)
                     /locus_tag="ACHJWB_004544"
     CDS             complement(4452..>4751)
                     /locus_tag="ACHJWB_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4316035.1"
                     /translation="RWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTY
                     RGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          977 a         1505 c         1210 g         1059 t
ORIGIN      
        1 ttctgatcat cactgattct taccgtgatg gtgccactgg cataactgtt cgtgcggggg
       61 aaagacttgc tgaccgtttt gacaatatcg ccttcaatct gattggctaa cagtttcccc
      121 ttaatctgac agttctcatt aatcgtgacg ttgttgagcg tcccggagtt cgcattcaca
      181 ctaccactga tatccgcatt tttagcagtc agctttccgt ccggtgtcag ggaaaatgcc
      241 ggaggactgc caccactggt aatggtgggg gccgtcaggc gcttcaggaa cacgtcgttc
      301 atgaatatct ggttgccctg cgccacaaac atcggagttt cattcccgtt tgccgggtca
      361 ataaacgcga tacggttagc ggcaaccaga aactggctca gcttgccttc ctccgtgtcc
      421 tccatgctga ggccaatacc cgcgacataa tgtttgccgt ctttggtctg ctcaattttg
      481 acgccccaca tggcattcca tttatcgtta gcgtccttcc actctttcga aaactcatcc
      541 agtctgctgg cgttatcctc cgtcagatcg actttttcca gcagctcctt gccgagatga
      601 gattcggtta tcttgccttt gaaaaaatcc agataacctt ccgcatcatc gctcgcccga
      661 ccgacggcct ccacgaatgc cgatttgcca acagtgttca cactgcggat ataaaagtaa
      721 taatcatggc ccggtttgat attgatactg gcggctatcc agtacagccc cgtgccaagg
      781 tagcgggctg tggtttcaac ctgcctgata tcggtaatcc gcttttccga gaaccagaac
      841 tcaaactgta ccgtcgggtc ataaacggca agatgcggcg tggcggttat ctgaaaatag
      901 cccggcgtca gctcaatcct cgacggtgct gccggtgcgg caatccggaa cgataccgac
      961 gccgggtctc cctgctgccc ccacgcattt accgcccgga ctgtcagcct gtagttcccc
     1021 agcgccagtt gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc
     1081 tcactgccat catccgctgt tacggtcaga cggagcagga agctcacgcc cttcaccacc
     1141 ttcggcgtgt cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggcg
     1201 gtcaggtgct gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca
     1261 aagtacgccc cgttatccac gatggcttct ttttcaggca catgctgcac ggcggtgatg
     1321 gcatacgtgc cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc
     1381 gtcggcagct tcagccccca cacgctgtat tcagcaacac cgtcaggaac acggctcact
     1441 ttcaccttca cgccgtcggt gacggactga acctccacgc tgatcggatt gccacttccg
     1501 tcaaccaggc ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagtgtc
     1561 agcgtccggg tctgactgtt taccgccagc acgcgcccgc cgatgctgat accggcatag
     1621 tcatcatcac agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg
     1681 cagaagtcca cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg
     1741 tgtgcctgcc cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa
     1801 cgggcaatgg cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg
     1861 tcaatccagt tcacctcaac ggcattatgg cggtctttca gggcgctaaa gctgtagcgg
     1921 aacggcgcgc catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc
     1981 gacggtcggt cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc
     2041 gccgagcaga aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta
     2101 caggtgatgc gcggctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg
     2161 ccgatgacat acagcgccca tttatccaca tccgccgcac caaggcgttt ccccatgccg
     2221 tagcgcggat gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgccggt
     2281 ttaaacgttc cgtcccagat accgctgtat tgccgcgtct gcgggttata attcgacggc
     2341 acctgcagaa tacgcccgcg cagatgataa ttacggctca cctgctggct gccgaactgc
     2401 tccgagtcca cctgcacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgata
     2461 atttcggtgt atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc
     2521 gtcatcctgc gcatccggat attaaacggg cgcggcggca ggttatccac caccaccgag
     2581 gccagatact gtgaagtggt tttaccctta atggtgatgt ctttttccgt cacccagcca
     2641 ccattacgtt gtatctgaac cagcaggcgg acttccgatg gattcctgtc cccctttgag
     2701 gtggtttcca ccagtgcctg cacgccgaaa gtaaaacgca gacggtcaat gtttgccgac
     2761 gtgatggtcc gggtgatcgg cgtgtcatat ttcacttctg taccgagcac cgtctcggag
     2821 ccggaggatt caaatccctc cggcgatgtc tgctcctgct caccggcccg gaacaccacc
     2881 gtgacaccgg agatattggt attcccctca ctgtccagca ccggcgtact gttcagcagc
     2941 acgcttttta atccatccac cggaccttca atcgcccttc gctgatggca tcgatcacac
     3001 tcagcagctg cgtggacttc aggttgtcct tcgcttcgcg cggggtatgc cccttactgc
     3061 tgcctttacc cattcgtcac gctccataaa cgacaaaacc gcccgcaggc ggtttcacat
     3121 aaaatgtttt gcatcagcga ccaatcacca caacctgacc accatcccct tcgtctgccg
     3181 tgctgatctc ctgagaaacc acacgtgacc ccacgcgcat ttcaccgtac agaacaggca
     3241 gaacattgcc ctgggcaacc atgttatcca gtgaggagaa ataggtgttc tgcttaccgt
     3301 tatccgttgt ctgtgtacgg ggagttctgg ctttcggtgc cagcatctgc gccacaccac
     3361 caagtaccat actggcaccg agagaaaaca ggataccggt cataccaccg gccccaatgg
     3421 ctgcccccca tgctgcaagg gtggctccgg cagtaaagaa tgatccggca atggcggcag
     3481 cccccagaac aatctggaat acgccccctg acttagcccc ggcgactctg ggaacaatat
     3541 gaatcacagc gccgtcaggc agaacctcat gtaactgcgc cgtcaatccg gacgtgctga
     3601 cgtcccgccc ggaaatccgt acctgatacc agccgtcgct cagtttctga cgaaacgccg
     3661 ggagctgtgt ggccagcgcc cggatggctt cagcccccgt tttcacacga aggtcgatgc
     3721 ggcggccaaa tcgttgtaaa tccccgtaaa ggcagatgcg cgccatgccc ggtgacgcca
     3781 gagggagtgt gtgcgtcgct gccatttgtc ggtatacctc tctcgtttgc tcagttgttc
     3841 aggaatatgg tgcagcagct cgccgtcacc acagtaaatg gcggcatgat tcggcaccga
     3901 tgaaccaaag cagcacagca gcacgtcgcc aggctgcgcc tctgtcagtg cgacacggta
     3961 aaaaccagtc gcctccatat tgtcaagata gagattctga ccgttacgcc accagtcatc
     4021 cccgcgatga aaatccggca tctcaatccc cgccagatga taagcatccc ggaacagcgt
     4081 gtaacagtcc gtcaccccgt gctcaaagcg ccgcccggtg agatgcggca cacagcggaa
     4141 cttatgaatc gtcccccggc agaccagcca ccacggcaaa tcactctgca cctgcagccg
     4201 ccggtcggcc tcactcagcc agggcagacc accggggtgg ctgtggacca gcgccacaat
     4261 ctcaccctgc atttctgcct gcagccagtc ttccggtgca atacgaaaat acgcctccgg
     4321 ctcaccggag atattcacgc aggggaaata tctttccccc tccggcgtgc ttaccacgaa
     4381 gccgcacgac tccgctggcg cacatcgccg ggcgtgcgcc agaatcgctg attctgtctg
     4441 tgtcatggta tttactgcga aagtttgtta atggaaagga agccgccaaa gttgccgacg
     4501 ttattgcgga acttacaacc gctcaggcat ttgctgcatt tatccttcgt gatatcggac
     4561 gttggctggt catattcatc cgcgaccgcc ggaccgtgat aaccgcactc atcgccgcga
     4621 taggtccagg tgcaggtgtt ggccagcatg atacgtcccg gaaaaacagc gccgtccgtt
     4681 tccgtcggcg tggacagtac aaaagaggca ctcaccgcgc tcagttcgct gcactgctca
     4741 atgcgccagc g
//
LOCUS       DAZFAH010000276         4688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFAH010000276 DAZFAH010000000
VERSION     DAZFAH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.278"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..417
                     /locus_tag="ACHJWB_004545"
     CDS             <1..417
                     /locus_tag="ACHJWB_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4316036.1"
                     /translation="NQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGN
                     KYRMKPSLNREFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTP
                     LRIYEGGALECILNRYKPAWQTFIYVYANSHKEENI"
     gene            417..1328
                     /locus_tag="ACHJWB_004546"
     CDS             417..1328
                     /locus_tag="ACHJWB_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316037.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGGYYRPVQIYINNAWRTVSSV"
     gene            complement(1363..1884)
                     /locus_tag="ACHJWB_004547"
     CDS             complement(1363..1884)
                     /locus_tag="ACHJWB_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4316038.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            2241..2426
                     /locus_tag="ACHJWB_004548"
     CDS             2241..2426
                     /locus_tag="ACHJWB_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316039.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGFSTSF"
     gene            2440..3000
                     /locus_tag="ACHJWB_004549"
     CDS             2440..3000
                     /locus_tag="ACHJWB_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4316040.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            3100..>4688
                     /locus_tag="ACHJWB_004550"
     CDS             3100..>4688
                     /locus_tag="ACHJWB_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4316041.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGL"
BASE COUNT         1223 a         1104 c         1199 g         1162 t
ORIGIN      
        1 aaccagatgc tggcagaacg ttttcctgat tcatcccgtc tgctgctgga agactgggag
       61 cgttatctgg ggttaccgga atgtgatatg accggcgcaa ccattcagga acgtcagcgt
      121 tatgcaggga ataaataccg gatgaaaccc tctcttaacc gtgaatttta tatccggttt
      181 gcggcagagt ttggttatga aatagatatt cagccatcac cggattcaca gtgggtcagt
      241 attgtcacga taaacagtga aaccggctac cggaatatga atgtgctgga tgatattctc
      301 accccgctgc gtatttatga gggtggtgcg ctggaatgta ttctgaaccg ttataagcct
      361 gcatggcaga cgtttattta cgtgtatgca aacagtcaca aagaggagaa tatttaatgt
      421 tccatgttga taataattcc ggcgtggcga atatgcctga actggctccg gcgcagagta
      481 ataccaccac ctggtttacc gaaggtgacg gacaaaaagg tatcagctgg attggtcagg
      541 actggctgaa tattctccag gccgaactgc tgaatattct ggctgaagcc agtattcagc
      601 cggataaggc gcagttaaac cagcttacgc tgtccattaa agccattatc gctgcgaatg
      661 ccttttcccg gaaaaacaac ctgaaagaaa ttgctgatgc cggtgcggaa gcccagcgtc
      721 tggcccgtgg ttatcttggt ctggggacgc ttgccacaaa aaacagtctt ggtcccgctg
      781 acgttaatgc cctggcgaag gatcagaacc ttgccgacct ggagaatgcg gaaaccgccc
      841 gtaataatct ggatgtttac agcaggagcg aaggtgataa ccgttacctg cgcagggacc
      901 agaatggcgc ggatatccag gacaaaggaa cctttatcga taacctcggt ttacgggaaa
      961 cggtaaataa ggctgctgat gccctgccat cggctggaac cgccgtggca gcaaacagac
     1021 tcgccacccc aagaaatatt aatggtgttc cttttgacgg aacgctggat atcaacatca
     1081 cgtcaggaat gacgcagtca accgcagatg gccggtatgt acagaatgtt cagcttggag
     1141 cacagagcta tcattcaccc ggaggcaatg aaatgtcatg gaattacagc gcaccttccg
     1201 gttgtatgct ttccggtatt aacgtgcagg aaaccggctc ccggtctgcg gacaatatcg
     1261 gtgggggcta ttatcgcccg gttcagattt atattaataa tgcctggaga acggtaagtt
     1321 cagtgtaaac cacagaaaag ggtgctgaat gcaccctctg aattattccg gtttttccgg
     1381 ccagggaatc gcctggtaat gcccctcact ggcaatatca ttaaagtgca tttctttaag
     1441 ctgtcggata tacgccatcc agcgcgtcag ctgtaattta ttttcatcgc taatcatgtc
     1501 cagttttaat tctgttttcc agtcatccac ggtttcgttg gcttcctgga gcagcgatga
     1561 gcgacgcttt tcagcaaccg tcttccagtt taccggcacc tttgatatca ggccatcaga
     1621 aaactgccag ttaccgtcaa tattcacacc ttccggcagt tcgtccactt caacaacagt
     1681 aaaacctgcc ggatataaag cagagacatc ttcagcaaca gagcagataa tacccgttac
     1741 aggcgaaata cagagtttgt atttctttgt aaaaagcggg atagattcat aaaaatccct
     1801 tccgtcctca ctctggaaat actgaacatc ctcaccatag ggtttgtctt tcgggtaata
     1861 tcgtttgaca ttaatcagtt gcataaacat caccatgtaa atcaatgata aaaacaggaa
     1921 taatactatc gccatataca ggcaatgctt gtctgaaata acgcatattt cagatgctgg
     1981 ctgctgtgtg ctatgccccc gttaattaaa tacttgcagc ggatacccaa tgcccattaa
     2041 tacaatattg tatctgtctg taataaacac ggtcatcacc accacgcatt tcccagtctc
     2101 cgtcagtctt aaaaccggta atcacatttc ctgatgacag ggagtaatta tattccagtc
     2161 cgccgcttaa tgcctgcgct cccagtctta cccctgtaac ataacgctga ttggccgttg
     2221 attccggcat tcctgacgta atgtttatat cctgcgtgcc gtcaaaggcg acaccattga
     2281 ttctgcgggc atttgccagc ctgcttgctg caacagccgt accgtttgat ggcagggcat
     2341 tcgcggccct gtttaccgta tcccgtaaac cgaggtttca gggatgccgt atatctgctt
     2401 taaaaggctt ttccacctct ttttaaggaa ggtaaataca tgctgattgg ctatatacgc
     2461 gtatcaacaa atgaccagaa caccggttta caaagaaatg cgctggaatg tgcaggatgt
     2521 gagcagattt ttgaagacaa aatgagtggt aaaacgacac agcggccggg actgaagcgg
     2581 gcactgaaga aactaaaacc cggtgacacg ctgatggtct ggaaactgga caggctgggg
     2641 cgcagtatgc gccaccttgt tgcgctgaca gatgaattac agcaacaggg gataaatttt
     2701 cgtagtctta ctgacaacat tgatacgtca acccccatgg ggcgcttctt ttttcacata
     2761 atgggtgcgc tggcagaaat ggagcgtgaa ttaattgtcg aacggacacg ggctggtctt
     2821 gcagctgccc gtgaaagggg acgaaccggc ggcagacccc gccgtctcac gcaggaacaa
     2881 tacgagcagg tcgggcgttt actggccggg ggtgtcagtc gaagacaggt ggcacttatt
     2941 tttgatatcg gtctttcaac cctttatcgt tattatcctg cgagcgtttc ggaaaaataa
     3001 gacgctctct ttgttgggcc tttactgaat taacgcaaaa tggcgtttat cggtctgtgg
     3061 atatgataat tcagtttatt caggaacagg ctgattatta tgacgtccaa atgggttcaa
     3121 ctatcatcaa tgcccggaaa ctttactgtt aaagtttccg gtggtacagc ggcatttctt
     3181 gaggctccct ttcctccggc cgaaacaaaa ggaggaatga catttgctga ctgtctcatc
     3241 agttttaata cccgggattg cctgtgggta aggccagtat ccggtgatcc gagtgtggaa
     3301 attaccggag cgggtattgg tgcggtcatt ccgttaagtg ctgatgttgc cggtactgct
     3361 gaaccgtcag actgggataa tgctgaaaca catacccgtc cgtcaggaaa tgaaactgct
     3421 tccagctccc cttcctggta ttacgtggtg gttcttgctg gtcagtcaaa cggtatggct
     3481 tacggtgaag gtctgccact gccggagaca tatgaccgcc ccgaaccgcg tattatgcag
     3541 ttagcccgtc gcagtacagt cacgccggga ggtaaagcct gtcagtataa cgacatcatt
     3601 ctggccgacc actgtctgca tgatgtgcag gatatgagtg gaaaaaatca cccgaaagcg
     3661 gatgtggcta aagggcaata tggcaccgtg ggtcaggggc ttcacattgc caaaaaactg
     3721 ctgccgttta ttccggcaga tgcggggatt ctgctggttc cctgctgccg tggtggttct
     3781 gcattcacgg cgggtgctga cggcacgtac agtgacagca cgggtgcttc agaggactca
     3841 gcccgctggg gtgtggataa gccgctgtat aaggacttaa tcagccggac aaaagcggca
     3901 ctggcgaaga acccgaaaaa ccgcctgctt gctgtggtgt ggatgcaggg cgagtttgat
     3961 attgatgcga agccgacgga gcattctgcg ctgtttctgg cgatggtgga aaaattccgc
     4021 gcagacctgg ctgaacaggc ggaacagtgt accggtggca gtgctgctgg cgttccgtgg
     4081 atttgtggcg ataccacgta tttctggaaa cagaaaaatg aaccagcata ccaggcaatc
     4141 tatggcggct acaaaaacaa aacagataaa aacattcatt tcgttccatt aatgacagat
     4201 gaaaatggcg tgaatgtacc caccaacaat ccggcagaag acccggatat tgaatccatt
     4261 ggttattacg gctcaacgtg gcgtaacagt gccgctacct ggacatctgc ggaccgggcc
     4321 agccatttca gtgcctgggc gcgccggggt attatttctg accgtctggc cacggctgtt
     4381 ctgactcatg cagggcgaac cacagtaaaa gccgatgttc catcttctga aactgaagca
     4441 ccagtgccgt caccttcaga aactgaagca gtaaccacaa cactgctgtc ttaccgtgtc
     4501 agtgagtcag aaggaaatct gaaagcccag ggctgggagc cggctggcgg taaagctgaa
     4561 attatcagtg atgcaggagg cacaggtggt aaggcaatga aactgaccaa ggaaacaggt
     4621 aaatcatcct ggtatctgga tcatgatgcc ggtactggtg ctgaactgct gaaaaatggt
     4681 ggtttaat
//
LOCUS       DAZFAH010000277         4675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFAH010000277 DAZFAH010000000
VERSION     DAZFAH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.279"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..501
                     /gene="repA"
                     /locus_tag="ACHJWB_004551"
     CDS             241..501
                     /gene="repA"
                     /locus_tag="ACHJWB_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4316042.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLTQLCKDSGLTQKEMIEHWILKEKAAMDDANRR"
     gene            complement(563..649)
                     /locus_tag="ACHJWB_004552"
     CDS             complement(563..649)
                     /locus_tag="ACHJWB_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO4316043.1"
                     /translation="MIGVFAFLAPVQHPHQNQFPATLRRGQP"
     gene            739..813
                     /gene="tap"
                     /locus_tag="ACHJWB_004553"
     CDS             739..813
                     /gene="tap"
                     /locus_tag="ACHJWB_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4316044.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            806..1663
                     /gene="repA"
                     /locus_tag="ACHJWB_004554"
     CDS             806..1663
                     /gene="repA"
                     /locus_tag="ACHJWB_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4316045.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGETVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2578..2847
                     /gene="yacA"
                     /locus_tag="ACHJWB_004555"
     CDS             2578..2847
                     /gene="yacA"
                     /locus_tag="ACHJWB_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO4316046.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVFPHDMVESSAAAWRDEMSRKVAGK"
     gene            2847..3125
                     /gene="yacB"
                     /locus_tag="ACHJWB_004556"
     CDS             2847..3125
                     /gene="yacB"
                     /locus_tag="ACHJWB_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO4316047.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(3361..4548)
                     /locus_tag="ACHJWB_004557"
     CDS             complement(3361..4548)
                     /locus_tag="ACHJWB_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO4316048.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTPDHTWKNLHFYARKVMSMWRYRITWLLSRKYPELVIPDALAVEGSSRR
                     DWNRFLDSHYRRGWNVNVSRVIDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGCQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
BASE COUNT         1165 a         1263 c         1184 g         1063 t
ORIGIN      
        1 ggtatttcag gggcagacac ccgacatgcc agaaacagcc ggtcccgccc ggggccggca
       61 tctaggttaa ggcatttcca gcttttcagt catttcatta tcaaaatcac attaaacggt
      121 cgtaatcaga catgatttgt gcgccaacac agatcatcgt cacaactttc aagtcgctga
      181 tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg aggaggcggg
      241 atgtcgcaga tcgaaaatgc agtaacttcc tcaccgaaac gcatttacag aaaaggtaat
      301 ccgttaaccg gagccgagaa acaacgtata tctgtttccc ggaaaaaggg gacacataaa
      361 gcgattaatg ttttcatcca gagtgaactc aaagatgatt tgacacagct ttgtaaagac
      421 tcgggcttaa ctcaaaaaga aatgatagag cactggattt tgaaagaaaa agctgcgatg
      481 gatgatgcga atagaaggta actaagttac tttcttgaac atttgaattg cggatgctag
      541 attactgatc gtttaaggaa ttttatggct ggccacgccg taaggtggca gggaactggt
      601 tctgatgtgg atgttgtaca ggagccagaa aagcaaaaac cccgataatc ttcgagaagt
      661 ttggcgacta agagaagatt accggggccc cataaaaccg tatagccaac aattcagcta
      721 tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg ctcgctcctt
      781 ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacgtatt accgccaggt
      841 aaagaacccg aatccggtgt ttacaccccg taaaggtgcc ggaacgctga agttctgcga
      901 aaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca ttcatgtggc
      961 gcatgcccgt tcccgtggtc tgcgtcggcg catgccaccg gtgctgcgtc gacgggctat
     1021 tgatgcgctg ctgcaggggc tgtgtttcca ctatgacccg ctggccaacc gcgtccagtg
     1081 ttccatcacc acactggcca ttgagtgcgg actggcgaca gagtccggtg caggaaaact
     1141 ctccatcacc cgtgccaccc gggccctgac gttcctgtca gagctgggac tgattaccta
     1201 ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgacatca cgttcacacc
     1261 ggctctgttt gctgcccttg atgtgtctga ggatgcagtg gcagctgcgc gccgcagtcg
     1321 tgttgaatgg gaaaacaaac agcgcaaaaa gcaggggctg gataccctgg gtatggatga
     1381 gctgatagcg aaagcctggc gttttgtgcg tgagcgtttc cgcagttacc agacagagct
     1441 taagtcccgt ggaataaaac gtgcccgtgc gcgtcgtgat gcgaacaggg aacgtcagga
     1501 tatcgtcacc ctggtgaaac ggcagctgac gcgtgaaatc tcggaagggc gctttactgc
     1561 taatggtgag acggtaaaac gcgaagtgga gcgtcgtgtg aaggagcgca tgattctgtc
     1621 acgtaaccgc aattacagcc ggctggccac agcttccccc tgaacctgac ctcctctgaa
     1681 taatccggcc cgcgccggag gcttccgcac gtctgaagcc cgacagcgca caaaaaatca
     1741 gcaccacata caaaaaacaa tctcatcatc cagcttctgg tgcatccggt ccccctgttt
     1801 ttgatacaaa acacgcctca cagacgggga attttgctta tccacattaa actgcaaggg
     1861 acttccccat aaggttacaa ccgttcatgt cataaagcgc cagccgccag tcttacaggg
     1921 tgcaatgtat cttttaaaca cctgtttata tctcctttaa actacttaat tacattcatt
     1981 taaaaagaaa acctattcat tgcctgtcct gtggacagac agatatgcac ctcccaccgc
     2041 aagcggcggg ccccgaccgg agccatttta gttacaacac tcaaatacaa ccaccgggaa
     2101 aaccccggtc cagcgcagaa ccgaaaccac aaagcccctc cctcataact gaaaagcggc
     2161 cccgccccgg cccttcgggc cggaacagag tcgcttttaa ttatgaatgt tgtaactata
     2221 ctaagtcatt gctgtcagta ttctggctgg aagtcctcag tacgcgctcg taagcggccc
     2281 tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt cgggaccact
     2341 ccgaccgcgc acagaagctc tctcatggct gaaaacgggt atggcttggc agggctgggg
     2401 atgggtaagg tgaaatctat caatcagtac cggcttacgc cgggcttcgg cggttttact
     2461 ccggtatcac atgtaaccac agcgtgccgc cttccatgcc gctggcgcgg catatcctgg
     2521 tgacgatatc tgaatcgtta tatactgtgt atatacgtag taatgacgag gtgataaatg
     2581 gcacaggtta atatgagttt aagaatcgac gctgaactga aggatgcttt tatggccgct
     2641 gcaaaaagca tggaccgtaa tggctctcag ttaatccggg attttatgcg ccagaccgtt
     2701 gaacggcagc ataatacctg gttccgtgac caggttgcgg caggacgtca gcaactcgag
     2761 cgcggcgatg tgtttcccca tgacatggtc gaatcttctg ccgctgcatg gcgggatgaa
     2821 atgagcagga aggtcgccgg taaatgatgg agatattctg gaccatgctg gccagccagg
     2881 acaggaagcg cattcgtgag tacattgcgg agcagaacct gatggccgcc attgaactgg
     2941 acgaacggat tggttattca gccagcagcc tggccgggca gccgtataaa ggccgcaacg
     3001 gtcgtgtgga aggtacccgg gaactggtta ttcacccgca ttttgttctg gtttatgagg
     3061 tcgacagcca gtggggaaaa gtgtatatcc tgcgtgtatt gcatactgca cagaagtggc
     3121 catagagggg ctgtccggat ataaaaacgt ctgacggaga tggggtccgc ttgatcgata
     3181 ggaatttaaa tcccaaaaag attaaaaaaa cacccaaaaa cggatgttta ttcaacactg
     3241 cctgcgtgcc atgagtgcac cgccgtcaat taaatggatc ttatcgattg atactgtata
     3301 tcactgatgg ttttttactc cattcccctg aaaatggacg caacttcccc gatgcggctc
     3361 tcagccgcac acccgctgtt gcgccagtgg ctcatgcatc aggaccagct ctgtcagacg
     3421 gtagccccgc ttcagccccg taaaacgcat ctgacatccg cacaggatgc acttcagcgg
     3481 gtcaaccttc agtaaccgct gatacatccc tctccacctg atttgcatcg ccgtctttct
     3541 caccgtctcc gttatgacgt acacaacatc ttccagtaac cggcgcttca ccggactcag
     3601 gaaaccgtag tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc
     3661 cataaactca tcaccactca tcaccaggta ttcttcccgt tttgtccggt gactgttgta
     3721 acgcagacca atttcatcct gaccagcata gtgctccaga cggctcatcg gcaccggcgg
     3781 ttttttcagg taagagccga agtacaccgc cacatgtgtg gcgttatcaa tcacccggga
     3841 tacgttgaca ttccagcccc gccggtaatg actgtccagg aagcgattcc agtcccgtct
     3901 gctgcttcct tcaactgcca gcgcatccgg tatcaccagc tccgggtatt ttcgtgacag
     3961 taaccacgtt atcctgtaac gccacatgct catcacctta cgggcgtaaa aatgaaggtt
     4021 tttccaggtg tggtctggtg tcacgccgcc ggccgttgtc gacaggtgaa tgtgcggatg
     4081 ccactgctgg tcacgtcccc atgtgtggat caccgtgaat atccccggca ccacatctgc
     4141 ctggcggcag atttcctgta tcacatccgc agcaatgcgg ctcatctctg ccagtaacca
     4201 ccggttgtgg aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca
     4261 cggacagtcg ggaaccagac tcagcagata ctgtatccac tgtgcgccag ccttcactcc
     4321 gcagtgcggg caggagcgac ttttacaccg gaagcagacc ttttttgtgt ggcaacagtc
     4381 cggagatgaa cagcaccact gtgtgtaccc catcagcgtg gttccgcatg ccatgatttt
     4441 tgtcaccgac tcaatgacca ccggacgcac agaaccttcc ggttgtttct ccagccagtt
     4501 aagccagcgg tttccctgct gaaaaatgtc ggcaaaacgg ggaagcatca gaagggcggg
     4561 gggactccgt ccggccagtg aaccgtgcca cactccgggc agtacatgcc gccggcgctg
     4621 ataccggcaa gaatggtcgc aaactcccgc tccgtgcagc gggctatttc aggat
//
LOCUS       DAZFAH010000278         4588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFAH010000278 DAZFAH010000000
VERSION     DAZFAH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.280"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..568
                     /gene="fimA"
                     /locus_tag="ACHJWB_004558"
     CDS             5..568
                     /gene="fimA"
                     /locus_tag="ACHJWB_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4316049.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            929..1639
                     /locus_tag="ACHJWB_004559"
     CDS             929..1639
                     /locus_tag="ACHJWB_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4316050.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATRDISF
                     QTINDYGALTPRIKATMQ"
     gene            1681..4331
                     /locus_tag="ACHJWB_004560"
                     /pseudo
     CDS             1681..4331
                     /locus_tag="ACHJWB_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            4345..>4588
                     /locus_tag="ACHJWB_004561"
     CDS             4345..>4588
                     /locus_tag="ACHJWB_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316051.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKG"
BASE COUNT         1307 a          990 c         1013 g         1278 t
ORIGIN      
        1 aattatgaaa ctcaaacatg ttggtatgat tgtcgtttct gtgttggcga tgtcgtctgc
       61 tgcggtaagc gcagccgagg gtgatgaatc agtaacgacc actgttaatg gcggtgttat
      121 tcattttaaa ggtgaagtgg taaatgccgc ttgtgcaatt gattcagaat caatgaacca
      181 aacggttgag ctgggtcagg ttcgttcttc tcgcctggct aaagcgggtg acctcagctc
      241 cgccgttggc ttcaatatca agctgaatga ttgtgatacc aatgtttcca gtaatgcagc
      301 tgttgcattc ctgggtacta ctgtcaccag taatgacgat acgttagcgc tgcagagttc
      361 agcggcaggc tctgcccaaa atgtcggtat tcaaattttg gaccgtacgg gtgaggtatt
      421 aatacttgat ggggccactt ttagtgctaa aaccgacttg attgatggca cgaatatact
      481 gccattccag gctcgttata ttgctctcgg gcagtccgta gctggtactg caaacgctga
      541 tgcgaccttc aaagttcaat atctataatt caatcttaag gtggctatac gtcatctgaa
      601 attgaaacag ggtgtgtgtg gcatggctac gcacacccga gcaatttacc aatattcatc
      661 tcatttttaa tgagaaatgg tttgtatttg gagcgctatg aaaaatcaca taatatctaa
      721 actatcaata aacaacagta gttaaactta ctgttattta aaatatttac tttgtagcaa
      781 ggttttccta ttaaaagaga accaacattt attacaaacg cattaagtct ggaaacccgg
      841 cgactcgcca atgtattagc aacattaatc gattgtctga tgtaacggtt ttcccgctta
      901 tcctcttaac acatggagaa tgcatcagat gcaaactacg cgtaccccct actctatttc
      961 ctttatggcg acagtgttac ttttattgct ctttgcttgc catagcaccg ttgccaacgc
     1021 agctgtcgca ctgggtgcga cgcgggtaat ttatccggca aaccagaaac aggttttatt
     1081 gcctgtcacg aataatgatc ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc
     1141 aggtgaccaa aaagacaccc agtttgtcat taccccaccg cttttttcca tgcaaggtaa
     1201 aaaagagaat acgctgcgga taatcaacgc tactaatcat cagttaccag gcgaccgtga
     1261 aagcctgttc tgggtgaatg ttaaagctat tcctgctatg gaaaaagacc agaagaatga
     1321 gaatacatta cagctggcaa ttattagccg cataaaaatg ttttatcgcc ccacgaacct
     1381 ggcaatggca cctgaagaag cgcctgcaat gcttcggttt cgtcgcagcg gtagcaaact
     1441 cacgcttatt aatccaacgc cctatttcat taccgtaaca aatatgaaag caggaaatag
     1501 caatctccca aacaccatgg tgccgccaaa aggtgaagta tcggtggata tcacgcatgc
     1561 tgcaaccagg gatatcagtt ttcagacgat caatgattac ggtgcattaa ctccgcgtat
     1621 taaggcaacc atgcagtaag ccagtgacct aattaataat aaactcaatc ggaattaata
     1681 atgacagcct ttcgtgccgc atttaaggca tatcgtatgc atcaggtgtt aataatgcct
     1741 cgctttgcac ggcttaccat tgcccttggc ttagcaactg cggtatttcc cgttgacgca
     1801 gagtattatt ttaatccacg gtttttaagc aatgatcttg ctgaatctgt cgatttatcc
     1861 gcctttacta agggacgcga ggcaccacct ggtacatatc gggtcgacat ttatttgaac
     1921 gacgaattta tgactagtcg ggatattact tttattgcag atgataataa cgcagaactc
     1981 atcccatgcc tgagtacaga tcttcttgtc agtcttggta taaaaaaata gcgttattag
     2041 acaataaaga acattcggca gaaaaacatg tgccggacaa cagcgcatgc acaccacttc
     2101 aggatcgttt ggccgacgca tcaactgagt ttgatgttgg tcagcaacat ttgtcactct
     2161 ccgttccgca aatttatgtt ggcagaatgg ctcgcggcta tgtttctcct gatctgtggg
     2221 aagaaggaat aaatgctggg cttctaaact atagttttaa tggtaattct attaataatc
     2281 gtagtaacca taatgcagga aaatccaact atgcatattt gaatttacag agtggcatca
     2341 acattggtag ttggcgactg cgcgataact caacgtggag ttataacagt gggagtagca
     2401 attcatctga cagcaataaa tggcagcata tcaatacgtc ggctgaacgt gacattattc
     2461 ccttacgctc acggttaacg gtaggtgata gttataccga tggcgatatt tttgatagtg
     2521 tgaactttcg tggcctcaaa ataaattcaa cagaagcgat gttgcccgat agccaacatg
     2581 gtttcgctcc ggtgattcat ggtattgccc gtggaaccgc acaagtgagt gtaaaacaga
     2641 acggatacga tgtttatcag actactgttc cacccggccc ttttactatt gatgatatca
     2701 actctgcggc caatggtggt gatctgcaag taaccataaa agaggcagac ggcagtattc
     2761 agacattata tgttccttat tcgtctgttc cggttctcca acgtgctgga tatacgcgtt
     2821 atgcgcttgc catgggggaa tatcgtagtg gaaataacct gcaaagctct cccaagttca
     2881 tacaaggtag cttgatgcat ggtctggaag gaaactggac accttatggc ggaatgcaaa
     2941 ttgcagaaga ttatcaggcc ttcaaccttg gtattgggaa agatttagga ctttttggtg
     3001 ccttttcttt cgatatcacg caggccaata cgacacttgc agatggcacc cgtcacagtg
     3061 ggcaatcggt taaatccgtc tacagcaaat ccttctacca gacaggaacc aatatccagg
     3121 tcgcaggata tcgctattct acgcaaggtt tttataactt atccgacagt gcctacagtc
     3181 gaatgagtgg ttacaccgtc aagcctccta ccggagacac caatgagcag acacaattta
     3241 ttgattattt taatctgttc tacaataagc gtggtcagga acaaataagc atctctcagc
     3301 agcttggaaa ttacggtacg acatttttca gtgccagtcg ccaaagttac tggaacacgt
     3361 cacgcagcga ccagcaaata tcatttggat taaatgtgcc gtttggtgat attacgactt
     3421 cgctgaatta cagctattcc aataatatat ggcaaaacga tcgggttcat ttactcgctt
     3481 ttacgcttaa tgttcccttc agtcattgga tgcgtacaga cagtcagtcg gcatttcgta
     3541 attcaaacgc cagttacagt atgtcaaacg atttgaaagg cggcatgacc aatctatcgg
     3601 gggtttatgg cactctgctg ccggataata acctgaatta tagcgttcag gtcggtaaca
     3661 cccacggagg taatacatcg tctggcacca gtggttacag ttctcttaat tatcgtggag
     3721 cttatggtaa tactaatgtc ggttacagtc ggagtggtga cagcagccag atttattacg
     3781 gaatgagtgg tgggattatt gctcatgctg atggcatcac ctttggacag ccgctgggcg
     3841 acacaatggt tctggttaag gctcctggtg ctgataatgt caaaatagag aaccagaccg
     3901 gaattcatac cgactggcgt ggctatgcca tattaccatt tgcgacagaa tatagagaaa
     3961 accgtgttgc tcttaacgcg aattcccttg cagataatgt tgaactgaat gaaaccgtgg
     4021 tcactgtcat cccaactcac ggtgctattg ccagagcaac atttaatgca caaatcggcg
     4081 ggaaagtatt aatgacgttg aagtacggta ataagagcgt tccattcggt gcaattgtca
     4141 cacacggaga gaataaaaat ggcagcattg tcgcggaaaa tggtcaggtt tatctgactg
     4201 gacttccaca gtcagggcaa ttacaggttt catggggcaa agataaaaac tcaaactgta
     4261 ttgtcgagta caagcttcct gaagtttctc ctggtacctt actgaaccag cagacagcaa
     4321 tctgtcgcta aggaacaata gattatgaaa tacaataaca ttattttcct cggtttatgt
     4381 ctggggttaa ccacctattc tgctttatcc gcagatagcg ttattaaaat tagcgggcgc
     4441 gtcctcgatt atggctgcac agtctcatcg gattcgctta attttaccgt agatctccaa
     4501 aaaaacagtg ccagacaatt tccaacgacc ggtagcacaa gtccagccgt cccttttcag
     4561 attacgttaa gtgaatgcag caaaggga
//
LOCUS       DAZFAH010000279         4474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFAH010000279 DAZFAH010000000
VERSION     DAZFAH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.281"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            159..1316
                     /gene="acrE"
                     /locus_tag="ACHJWB_004562"
     CDS             159..1316
                     /gene="acrE"
                     /locus_tag="ACHJWB_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4316052.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1385..4431
                     /gene="acrF"
                     /locus_tag="ACHJWB_004563"
                     /pseudo
     CDS             1385..4431
                     /gene="acrF"
                     /locus_tag="ACHJWB_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1075 a         1092 c         1189 g         1118 t
ORIGIN      
        1 tttttgtgtt tatgtgcctt gagacgccag tattcataac tattccttac atcgacgaat
       61 gataatttgt aggatagcaa actgtatttt tctttctgcg agttaacgcg ttgcctttct
      121 gggtaaataa cgcgcttttg gttttttgag gaatagtaat gacgaaacat gccaggtttt
      181 tcctcctgcc ctcctttatt ctgatctccg cggctttaat cgccggttgt aacgataagg
      241 gagaagagaa agctcacgtc ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc
      301 cgttagaagt taagactgaa ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc
      361 gcccacaggt tagcgggatc gtactgaatc gtaatttcac tgaaggcagc gatgtacaag
      421 caggccagtc cctgtaccag atcgatcccg cgacctatca ggcaaattat gacagcgcga
      481 aaggcgaact ggcgaaaagt gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt
      541 acgttccgct cgtgggtacg aaatacatca gccagcagga gtacgaccag gccattgctg
      601 atgctcgcca ggccgatgcc gccgtgattg ccgcaaaagc cactgtcgaa agcgctcgca
      661 tcaatctcgc ttataccaaa gtgactgcgc caattagcgg acgtatcggc aaatcgaccg
      721 tgaccgaagg cgctcttgtc actaatgggc aaacgactga actggcgact gtccagcagc
      781 tcgatcctat ctacgttgat gtgacccaat ccagcaacga ttttatgagg ctgaagcaat
      841 ccgtagagca aggaaatttg cataaggaaa acgccaccag caacgtagag ttggtcatgg
      901 aaaacggtca aacctatccc ctgaaaggta cgctgcaatt ctctgatgtg accgttgatg
      961 aaagcaccgg ctccataacc ctacgtgctg tcttccctaa cccgcaacat acgcttttgc
     1021 cgggtatgtt tgtgcgtgca cggattgatg aaggcgtcca acctgacgcc attcttatcc
     1081 cgcaacaagg cgttagccgc acaccgcgtg gtgatgcaac cgtgctgatt gttaacgata
     1141 aaagtcaggt ggaagcgcgc cctgtcgttg ccagtcaggc gattggcgat aaatggttga
     1201 ttagcgaagg tctgaaatct ggcgatcaag tcattgtcag cggcctgcaa aaagcgcgtc
     1261 cgggagagca ggttaaagcc actaccgata cccccgcaga tactgcatcg aagtaaggta
     1321 atctgacatg gcaaactttt ttattcgacg accgatattt gcatgagtgc tggccattat
     1381 tctgatgatg gcgggcgcac tggcgatcct acaattgccc gtcgctcagt atccaaccat
     1441 tgcaccgcct gcggtttctg tttcggcaaa ctatccaggt gctgatgcgc agaccgtgca
     1501 ggatacggtg acgcaggtta tcgaacagaa tatgaatggt atcgataacc tgatgtatat
     1561 gtcctccacc agcgattccg ccggtagcgt gacaattacc cttaccttcc agtccgggac
     1621 cgatcctgat atcgcgcaag tgcaggtgca gaacaaactc cagctcgcca cgccgttgct
     1681 gccgcaggag gttcagcagc aggggatcag tgttgaaaag tccagtagca gctatttgat
     1741 ggtggcgggc tttgtctctg ataacccaga caccacacag gacgatatct cggactatgt
     1801 ggcctctaac gttaaagata cgcttagccg tctgaatggc gtcggtgacg tacagctttt
     1861 cggcgcacag tatgcgatgc gtatctggct ggacgccgat ctgctaaaca aatataaact
     1921 gacaccggtt gatgtgatta accagttgaa ggtacagaac gatcagatcg ctgccggaca
     1981 gttgggcgga acgccagcgt taccagggca acagttgaat gcctcgatta ttgctcaaac
     2041 gcggttaaaa aatccggaag aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc
     2101 ggtggtacgc ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact ataacgttat
     2161 cgctcgtatc aacggaaaac cggcggcggg cctggggatt aagctggcaa ccggcgcgaa
     2221 tgctctcgat accgcgaaag ccattaaggc aaaactggcg gaattacagc cattcttccc
     2281 gcagggaatg aaggttctct acccttatga caccacgcca ttcgtccagc tttctattca
     2341 cgaagtggta aaaacgctgt tcgaagccat tatgctggtg ttcctggtga tgtatctgtt
     2401 cttgcagaat atgcgagcaa cgctgatccc caccattgcg gtacccgtgg tgttattagg
     2461 gacgtttgcc atcctcgccg cttttggtta ctccatcaac acactaacga tgttcgggat
     2521 ggtgcttgcc atcgggctgc tcgtcgatga tgcgatagtg gtggtggaga acgtcgagcg
     2581 cgtgatgatg gaggataagc tcccgccaaa agaagcgacg gaaaaatcga tgtcgcaaat
     2641 tcagggcgca ctggtgggta tcgcgatggt gctgtcagcg gtatttattc cgatggcatt
     2701 cttcggcggt tctactgggg caatttatcg ccagttctct atcaccatcg tttcggcaat
     2761 ggcgctttct gttctggtgg cattgattct tacccctgcg ttatgtgcaa cactgctcaa
     2821 acccacctca gctgagcatc acgaaaataa gggtggtttc ttcggctggt ttaataccac
     2881 cttcgatcat agcgttaacc actacaccaa cagcgtcggc aaaatcctcg gttccacagg
     2941 acgatattta ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt ttttacgtct
     3001 tccgtcttcc ttcttacctg aagaggatca gggtgtcttt ctgaccatga ttcagttacc
     3061 cgctggcgcg acgcaagagc ggacgcaaaa agtgttggat caagtgacgg attactatct
     3121 gaagaacgag aaagcgaacg ttgaaagtgt ctttacggtt aacggcttta gcttcagcgg
     3181 ccaggcacaa aacgccggta tggccttcgt cagtctgaac cgtgggaaga gcgtaatggt
     3241 gatgaaaaca gtgcggaagc ggtaatccat cgtgccaaaa tggaattggg caagatccgc
     3301 gacggttttg tcattccatt caatatgcca gccattgttg aactgggcac ggcaacgggt
     3361 ttcgactttg agttaattga tcaggctggg ctgggtcacg atgccctgac ccaggcccgt
     3421 aaccagttgc ttggcatggc ggcgcaacat cctgccagct tagtcagcgt gcgtcctaat
     3481 ggcctggaag acaccgcgca gtttaaactg gaagttgacc aggaaaaggc gcaggcatta
     3541 ggtgtttcac tttctgacat caatcagacc atttcaacgg cgttgggtgg gacttacgtt
     3601 aacgacttca tcgaccgtgg tcgcgtgaaa aaggtgtatg ttcaggcgga tgccaaattc
     3661 cgtatgctgc cagaagatgt cgataaactt tatgtccgca gcgccaacgg cgaaatggtg
     3721 ccattctcgg cctttaccac ttcacattgg gtgtatggct ctccgcgact ggaacgctac
     3781 aacggtctgc cgtcaatgga gattcagggg gaagccgcgc caggaaccag ttccggcgat
     3841 gccatggcgt tgatggaaaa ccttgcgtca aaattacctg cgggcattgg ttatgactgg
     3901 acgggtatgt cgtatcagga acgcttatcg ggaaaccagg ctcccgctct ggtagcaatt
     3961 tcctttgtgg ttgttttcct gtgccttgct gcactctatg aaagctggtc aattcctgtc
     4021 tcggttatgt tggtcgtgcc gttagggatt gtcggcgtgc tgctggcggc gacactcttt
     4081 aatcaaaaaa atgacgtcta ctttatggtg ggcttgctaa cgacaattgg cttgtcggcc
     4141 aaaaacgcta ttttgattgt tgagttcgct aaagatctca tggagaaaga gggtaaaggt
     4201 gttgttgaag cgacactaat ggcagtacgt atgcgtctgc gtcctatcct gatgacctct
     4261 ctcgccttta ttctcggcgt attaccgcta gctatcagta acggtgccgg cagtggcgcg
     4321 cagaacgcag tgggtatcgg ggtaatgggc ggaatggtct ctgcaacgtt gctggcaatc
     4381 ttcttcgtac cggtgttctt tgtggtgatc cgccgttgct ttaaaggata aatcagaaac
     4441 ataaaggcac tttcgggtgc ctttattatt ttca
//
LOCUS       DAZFAH010000280         4412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFAH010000280 DAZFAH010000000
VERSION     DAZFAH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.282"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..784
                     /locus_tag="ACHJWB_004564"
     CDS             44..784
                     /locus_tag="ACHJWB_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4316053.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(905..2884)
                     /locus_tag="ACHJWB_004565"
     CDS             complement(905..2884)
                     /locus_tag="ACHJWB_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO4316054.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGV
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(2895..4295)
                     /locus_tag="ACHJWB_004566"
     CDS             complement(2895..4295)
                     /locus_tag="ACHJWB_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4316055.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIARAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         1051 a         1151 c         1192 g         1018 t
ORIGIN      
        1 atgccgactc ttttttatct tattttaaaa tggaataccg gacatgttaa ttaatcgcaa
       61 tattgtggcg ttatttgcgt tgccttttat ggcaagcgca actgcttctg aattatccat
      121 tggtgctggt gcagcttata atgaatcgcc ttatcgcggt tataatgaaa atacaaaggc
      181 aattccgctg attagttatg aaggtgattc tttttatgtt cgtcagacca cattaggttt
      241 tattctgtcg caaagtgaaa aaaatgaact tagcctgacc gcatcctgga tgccgctgga
      301 atttgaccct gccgataatg acgatcatgc catgcaacaa cttgataagc gtgatagtac
      361 ggctatggcg ggggttgcct ggtatcacca cgagcgttgg ggaaccgtga aagcctctgc
      421 ggctgcggac gttctggata acagcaacgg ctgggtgggg gagctatcgg tattccacaa
      481 aatgcagata ggtcgcctgt cgctgacacc tgcgctgggc gttctctatt atgacgagaa
      541 tttcagtgac tattactatg gcatttcaga gagtgagtcc cgtcgtagcg gtctggcaag
      601 ttattccgcg caggatgcct gggtgcccta tgtcagcctg acggcaaaat acccgatagg
      661 agagcacgtc gtattgatgg cgagcgcagg atacagcgag ctgccggaag agattaccga
      721 cagcccgatg attgatcgta atgagagttt tacctttgtc accggcgtga gctggcgttt
      781 ttaattcacc ggtggatgtc ggtgcggctg gcctacgctc tcgcaccgac tcgtatgcct
      841 gataagacgt gttaagcgtc gcatcaggca atgtgctccc tacgttggat gcggcgcgag
      901 cgccttatcc aacctccgga tggcgatgct tttcctcatt cacccagatc cgcatttcgc
      961 cttcgccccg gttagcccag ctaaaccacg ggataaacgt cagagtttgc ggctggcgtt
     1021 tggctggcgc gctgtcgtaa tgccacagcg gttgctgctc tggattgctc tgttcatacc
     1081 ggtaacccgg tgcctggatt aagatcttat ggctaaacaa tcccttgcct tcaaatgtcg
     1141 taaatggcgc atcggtgggc agccacagat tatgcagtga ctcgccgttg tcggcctgtt
     1201 ccaggcaata caccagcggg ccgcgctgaa tcgccacttt tccggcgacg tgacgcacca
     1261 gcgggttacc gtaaacgcgg cgtaccggca tcggcaaagt cagattcagc gtatcgccct
     1321 cctgccattc gcgggtaatg tgcaaatacc ctttacgaat atcctgctcg acctcttccc
     1381 cattcaatat gatctgcggc tgtgtgcacc agtccggcag acgtaaagcc agcgtatgac
     1441 gcaccggctg gggcgattcg accgcaattg tcacctgctc ctgccacgga tagttcccgc
     1501 taacccgcag gcgcagtgtg ccattttcta ccggcacttc catgctgttt cctgcgtata
     1561 tgttgatata caacgcatct tcacgcggcg tgtagagata atgaccaatc gaggtcagca
     1621 cgcgggcgat atttggcgga caacaagcgc agccaaacca acgctggcgg atcggtttaa
     1681 cgtgatcgta gatatggttg aatttcagcg attttggatg tacttccagc ggattcacat
     1741 agaagaaatg tttgccgtcc agcgccatgc cgccgagcac ggtgttgtac agtgcgcgct
     1801 ccatcacatc ggcatattga ctgtcgcctt ccatttccag cattcgtcgg gcaaacatca
     1861 tcaggccgat ggaggcgcaa ctttcagcgt ataccgtgtc attcggcaga tcgtaatcgc
     1921 tgctgaacgc ttcaccgctg ctctgtgagc caatgccgcc ggtaatatat aactgacgct
     1981 gggccatatt gttccacagc cgcaggcagt cctgacgctt gctgtcatcg tgacttaaac
     2041 gcgcgagatg cgcgacgccg gtcatcagat agacaaaacg taccgcgtga ccgatggcgg
     2101 tttgctgttg cgcaaggggc aaatgtgcct ggctgtaggc tttgtctttc accatccacg
     2161 ccgggccgta ggtgtgccag tgcgaggtct gcccgcgctt ttcatattcc tggtcgtaat
     2221 agtgcggttg cacaccacgc tgttcgacaa aatagttcgt cagcgccagg tagcgcggct
     2281 cttcggtgac ttcgtagaga cgcatcagcg ccagttcaat ttccggatga ccgggataac
     2341 cctgcagctt gtcttcatct ggaccaaata cgcggtcgat atgatcggcc agacggcaaa
     2401 ccacctccag caagcgtcgt tttcccgtgg cctggaagaa ggcgactccg gcttcaatca
     2461 gatgaccggc gcagtaaagt tcatgacact ccgcaagatt gctccagcgt tcttcgggtg
     2521 cttttaccgt aaagtaagta ttgagatagc cgtcttcgca ttgggcggag gcgatcagtt
     2581 cgattacctc gtcagcggtt ttttccagtt cggcatcagg cttctggcac agcgaccagg
     2641 ctaccgcttc cagccatttg gcgacgtcgc tgtcctgaaa caccatcccg taaaattcac
     2701 cctcctgaag tccggcggca atgcgaaagt tttcaatcgc atggctgggt tccgcttctg
     2761 ggatacgatc gttcaaggca tcccattgat aaggaatcac cacgtcgcgg accagttgtt
     2821 ggtactgacc gaggaacgga tcgctgaccg tcagtttatg cagatcgact tccgaaatgt
     2881 tcatcttgtg ctccttaagg ctgtacatga cgctgacgca ggtcggtagc tatgcgtgac
     2941 atcataggat tattgagttt gcaaaagcgg atcgcccctg ccagcagcag gtggaatagc
     3001 gcaggcagta atgtttccat cgccgcgatg ccgccaagcg aactggcggt ctggttttcc
     3061 acgccaggct ggtaggcgac aaaaataaac agcaggctga tgatcccggc gctggaagcc
     3121 caggccagct tgatgaaaaa cagattgaag gcgaagttca tgccggaaga acgtacgcgg
     3181 gttttccact cgccgtagtc atcggcaaag gccatcaatg agaagtgcaa tggcaatgtg
     3241 aagccaagaa tcacgccatt accgaggatc accgccagcc acagcgtttg ataagccggg
     3301 ccggagggca ggaaccacat caacaccgcc agtgcagcga ggagcaggtt ggtgtagtaa
     3361 taaattttga cggtatcgaa acgccgcgtt aacgggctga caatcaccga gccgataatg
     3421 gaggcgaagg tgaccatggt gaagaacagc gacgtgtagc ccgtactgcc ttgtaagacg
     3481 taggtaatga agtacatata cccaccgccg cgaatattaa agacgttaat cagcaggaaa
     3541 gacatgacca gcatcagcag cagttggtcg ttgttccgca gcccggcaag atgttcgcgc
     3601 agggtaaatt tccccattgt ggagagcggc acccgttcac gaacccagaa aaaacagcac
     3661 aggaacatca ccacggcaat ggcgcacaat acgccgacgc ccagttgata gccgcgtgca
     3721 gcgttgccct gaccgaagag atctaccatc cacggtaagc ctaccgaaac cagaaatccc
     3781 gctacgccgc agagaacgaa tcgccaggac tggcaggaga tcacttcatt gtggcgggtg
     3841 gtcatggtgt tgatcagcgc gcagtacggt acgttgatgg cggtataacc gactgaaagg
     3901 agcaggtaag taccgaacgc ccgggcgatt tttactccca tgctggcatc tggcacggtg
     3961 aacgtcagga tgccgataat gccgatggga atggcaatcc atagctgcca cggacgaaag
     4021 cgcccccatc ggctttgcgt ccggtcggca atgacgccca tcaccgggtc ggagatggca
     4081 tcaaacacgc gcagtgcaat aaacagcgtg ccaaccagcg ccggagttaa tccaaaaacg
     4141 tctgtgtaga aaaaggtgag aaaattcatg atcaggcaag ttattacggt gccgcctgcg
     4201 tcacccaggc cgtagcctat tttttcccgt acagataacg catcgtcaat tttaccgtta
     4261 gcgatatcag cggtagtaat cggtgtagag gtcatagata ttcctcgtga atagggagca
     4321 atttttagtt ttattgaaat gacctgatta attctgccgt gaacgggaat atcaacaagg
     4381 cgagaaaaga gtaaaaaaag gtgacgattc cg
//
LOCUS       DAZFAH010000281         4406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFAH010000281 DAZFAH010000000
VERSION     DAZFAH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.283"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..192)
                     /locus_tag="ACHJWB_004567"
     CDS             complement(<1..192)
                     /locus_tag="ACHJWB_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4316056.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGF"
     gene            complement(195..914)
                     /gene="sanA"
                     /locus_tag="ACHJWB_004568"
     CDS             complement(195..914)
                     /gene="sanA"
                     /locus_tag="ACHJWB_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4316057.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1064..1948)
                     /gene="cdd"
                     /locus_tag="ACHJWB_004569"
     CDS             complement(1064..1948)
                     /gene="cdd"
                     /locus_tag="ACHJWB_004569"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4316058.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2078..2773)
                     /gene="yohK"
                     /locus_tag="ACHJWB_004570"
     CDS             complement(2078..2773)
                     /gene="yohK"
                     /locus_tag="ACHJWB_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4316059.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2770..3168)
                     /gene="yohJ"
                     /locus_tag="ACHJWB_004571"
     CDS             complement(2770..3168)
                     /gene="yohJ"
                     /locus_tag="ACHJWB_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4316060.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3407..4357
                     /gene="dusC"
                     /locus_tag="ACHJWB_004572"
     CDS             3407..4357
                     /gene="dusC"
                     /locus_tag="ACHJWB_004572"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4316061.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1163 a         1126 c         1139 g          978 t
ORIGIN      
        1 gaagccgata tatgccacca tataagcgaa aaagagtccg ggattttttg ctcgccagac
       61 atacaccggt tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc gccagccttt
      121 ccatgcttcg cggaaacaaa atatcgggat aaggaaaaaa acggcaacga caaaaaactg
      181 ctgtacatcc ataactactt tccttgtttc ttttgcaatt caagtaactg ttcaggcgtg
      241 acggcgggat acccctgcgc atcttccggc acctggtgca tagccggaat agggaccagc
      301 ggccctaaaa accgcggttc acgtttaaaa atataaaggt cagccagcgc accgaaacgg
      361 gcggcaaatt cgcgaatacg tactgacagc atatctttcg gtgacggtac ggcataacac
      421 tgagcctgaa tccccatatg cagcgcaata aataatgctc gctcacagtg gaaacgttgg
      481 gtgataataa tgaaatcatt agtatcgaaa actttgcgtg tacgcacgat ggaatccagc
      541 gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt cgacaccagc agcgattaaa
      601 tctttgcgca tggtcatcgg ctcattataa ctttgcaatg cgttatcgcc gctcagtaat
      661 agataattta ccttaccgct gttataggca ttaatcgctc cttgaatgcg gtagcgataa
      721 tactgattaa ttacgccagt acgataatat tttgctgttc cgagcaccac accgacctgg
      781 cggtagggga gatcctgcaa ttcgtcgtag atataaggcg cggttttcca gctcatccag
      841 cgatcgaggc cgagcacagt caacagcagc aagccgatca ggactaacag gctgaggaac
      901 acgcgcttta acatgcagga gactcgatct aaggaaaatt tccatcaggc tactttaccc
      961 gctggttttg cgcaagaaac ggcaaactgt ttgccgggat tttcgacacc aggagagggc
     1021 tggcaagccg gacaaggcgt tcacgccgca tccggcacca ggcttaagcg agaagtacgc
     1081 ggtcgatact gtgacagccg agagctttca gtgttgcgga ggtggcatcc cactgaatca
     1141 acggcgcatc ggctttttct gccagaaccg cgcgctggat atccgggtaa tcataaccct
     1201 tgagattcaa tagaattaac gctccctgca acggtggcag agtcgggttg aatgcggcgt
     1261 tttcagcgta gctgccgctg aaaatacgac cgtctttaca ttccagcgcg acaccgcttg
     1321 gcgacttact gtaaggcatg tgcgaacggt ttgccgctgc aatcgccgcc tgagaaagcg
     1381 catcaccagt cagcgcatag ccgtgatcct gctcgtccat cagcagcgtt ttaatctcca
     1441 gatctttcgg cccaaaggca tccggcagat agtcacgcag cgcgtgtgcc tcgcggcccg
     1501 gcagatgaat acgcagatcc agaccgctgt tcagttcatt cataaactga cggcagtgac
     1561 cacaaggcgt gtagttaacg gtgatggctg caagcgcttt ttcaccactc aaccaggcgt
     1621 ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat tgtcgcacca ataaattcca
     1681 tattggcacc gaaataccag gttccgctca caccgcgcgc aattgcgcca acattaaaat
     1741 tcgacaatgg cgtacgcgca caggccgccg ccagcggaag tagtgcgaat gccagcgcgt
     1801 cttcgtccag ccccgttgcg ctcttcagcg atgagacttg ctccccggtc aacaaagcgg
     1861 ggaagtactt gtctgccaga ataggttcca gtgcagattg caagttatcc gcaagttggg
     1921 caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta atgggataac
     1981 agtttacgaa cccgttatgg ctttatatgt gatctgaatc tcattaatta tgcaacgcgt
     2041 gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta gcccattact gccagaataa
     2101 tcgggaaaag gaacggtgcg atcagcgaag taattatccc gcataacacc agcgctagcg
     2161 aactaaatgc accttcctga taatccagct cggcgcagcg cgccgtaccg agggcgtgcg
     2221 aggcagtccc cattgccaga ccgcgcgcag ctttggtacg aatacgcatc gcattaagca
     2281 atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg
     2341 ccggaatacc gccaatactg ccgccaaccg ccattgcaat aggcgtggtg actgatttcg
     2401 gcaggattga cgcggcgatt tccggtgaag cgcccatcaa caatgccacg gaagtccccg
     2461 tcaccatcgc aaccacgctg ccgataaaac agatggtgat gatcgatttc cagcgcgcgc
     2521 ggatctggtg tagctgctca tataaaggat aggccagcgc gacgaccgcc ggttgcagca
     2581 gatcgtttag cacttcgcta cctttaaagt agctgtcgta agagatgccg gtcagcatta
     2641 aaaaaggaat gatcaccacc attgctacca gcaacgggtt aagcagggga aacttatacc
     2701 gtgccgccag tttgcgggcg gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga
     2761 tattcgccat cattcttctg atcctttctg acctacgact ttacgttcac cgtgtaccag
     2821 ttgcgaactc cagctcacca ccagaaaaac caccagcgta ctgattgcac aggaaaccac
     2881 taccgggcca aactgtgcgc ggagcaaatc aaaatattgc atgacgccta cgccaatcgg
     2941 cacaaacaat agcgccatat agcgaatcag tacgtagcac cccggattga cccatttcgc
     3001 cggaagaatt tgcaaggcca gcaggacaaa caggatcagc atcccgatga tgctgcccgg
     3061 aatggttacc ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg cataaatcag
     3121 gacgaaagcg cgtaaatatt gccagataat gttcagtgtc ttactcataa tcaacacccc
     3181 agatgaaatg gattcatcat acaattaatt cgtaaaacgt gctaccgatc acattaagaa
     3241 atcagcgctt attttttcgc tccccggcat tacgcaatgc gcaacgaact caggcataca
     3301 tcaagcatag agagggatta cagtttgcag caatccctct ttgcagacct ttaatccgtt
     3361 gaccataggc gctaacatgg cgcctcattt ttttgcgggt gaagatatgc gtgtgttact
     3421 ggctccgatg gagggtgtgc ttgactctct ggtgcgtgaa ttgctgaccg aagttaacga
     3481 ctacgatctg tgcatcaccg agtttgtccg cgtggtggat caactgctgc cggtaaaagt
     3541 ctttcatcgc atttgccctg agctacaaaa cgccagccgg acaccatctg gtacgctggt
     3601 gcgcgtgcag ttgttaggtc agttcccaca atggctggca gagaacgccg cccgtgcggt
     3661 ggagttaggt tcctggggcg tggatctcaa ttgcggctgc ccgtcgaaaa cggttaacgg
     3721 tagcggcggc ggcgcgacgt tactcaaaga tcctgaactc atctaccagg gtgcaaaagc
     3781 gatgcgtgaa gctgtaccgg cgcatttgcc cgtcagcgtg aaagtgcgtc tgggctggga
     3841 cagcggtgag aagaaatttg aaatcgccga tgcggttcaa caggctggtg ctacggagct
     3901 ggtggtgcat ggacggacaa aagagcaggg ttaccgcgcg gagcatattg actggcaggc
     3961 gattggcgag attcgccagc ggctgaatat tccggtgatt gccaacggcg aaatctggga
     4021 ctggcagagc gcgcaacaat gcattgcgat cagcggctgc gacgcagtga tgattggtcg
     4081 cggggcgctc aatattccca acctgagccg ggtagtaaaa tataacgaac cgcgaatgcc
     4141 gtggccggag gtggttgctt tgctgcaaaa atatacccgt ctggaaaagc agggcgatac
     4201 cgggttatat cacgttgcgc ggattaaaca gtggttgagt tatttgcgta aagaatacga
     4261 tgaagcaacg ggattatttc agcatgttcg ggtgttgaat aattcccctg atattgcaag
     4321 ggctattcag gcaattgata tcgataagct gcgataatag tggaatcgca gtttatcaga
     4381 gacaatgaat cgcttattca gcttca
//
LOCUS       DAZFAH010000282         4208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFAH010000282 DAZFAH010000000
VERSION     DAZFAH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.284"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..125)
                     /locus_tag="ACHJWB_004573"
     CDS             complement(<1..125)
                     /locus_tag="ACHJWB_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316062.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQ"
     gene            complement(122..346)
                     /locus_tag="ACHJWB_004574"
     CDS             complement(122..346)
                     /locus_tag="ACHJWB_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316063.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            complement(343..1245)
                     /locus_tag="ACHJWB_004575"
     CDS             complement(343..1245)
                     /locus_tag="ACHJWB_004575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4316064.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLKLSTAMYDAPASFLSSAFAHIQIMVGWAGAPKGAPVSVDAGYANPVQFTTSEIGVS
                     GGGSFSLSTEAAIMATIQALTQPEITIDNGQAVTTSLAVANFFSKRHDDVLKKIRTLD
                     CSPEFCARNFAETSISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYIN
                     AFNQMEKLLSKPSTLSDAADNASVLYSHLSVIHKVWLQQLYPMLAKAESPLAVSLYDY
                     INDASALACLINLSLNPSEVRGRK"
     gene            complement(1175..1354)
                     /locus_tag="ACHJWB_004576"
     CDS             complement(1175..1354)
                     /locus_tag="ACHJWB_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4316065.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            complement(1500..2051)
                     /gene="ymfL"
                     /locus_tag="ACHJWB_004577"
     CDS             complement(1500..2051)
                     /gene="ymfL"
                     /locus_tag="ACHJWB_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4316066.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQFFPLGWAMVLQRAAGTHHIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSNARECAAPGVVAFCVCGETNA"
     gene            complement(2044..2304)
                     /locus_tag="ACHJWB_004578"
     CDS             complement(2044..2304)
                     /locus_tag="ACHJWB_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316067.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEISEMQILYPARFQSSQNRNELKPQEQEVSRG"
     gene            2402..3094
                     /locus_tag="ACHJWB_004579"
     CDS             2402..3094
                     /locus_tag="ACHJWB_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4316068.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSISEDIAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            3817..4179
                     /gene="yfdP"
                     /locus_tag="ACHJWB_004580"
     CDS             3817..4179
                     /gene="yfdP"
                     /locus_tag="ACHJWB_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO4316069.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
BASE COUNT         1142 a         1031 c         1004 g         1031 t
ORIGIN      
        1 tgctggtagc tgggccagca ctcaccctga tcgctggcat tatcagccag cttgatcaga
       61 accagttttc gcaatggatt acccactcga attttcatcg ctttaaccat cagctccata
      121 ctcatgctgc acctccgaga tgcttcatgt tttttccgga gcgaaaggct ataagcggca
      181 tactgacgcg gtaattacgg ccaagcggtt cacaaatcac cttctgacat tcacggtcaa
      241 ccaggctaac acgtagaaca tgccctgcag gtgtggtgta ccactgaccc ggacgaggac
      301 aacggaaagt ctgattggta aaccgtttga aaatattccg gatcatttgc gcccccttac
      361 ctctgaaggg ttcagcgaca aatttatgag gcaggccagc gccgaagcat cattaatata
      421 atcatataag ctaacagcca gcggagattc agcttttgcc aacataggat aaagctgctg
      481 cagccagacc ttgtggatta ccgacaggtg ggagtaaagc acgctggcgt tatctgcggc
      541 atcgctcagc gtggatggct ttgaaagcag tttttccatc tggttaaagg cattgatgta
      601 tgcctctttg aaccgggcag cacgtttacc cgtgaaaccc atagcaagaa acgcaaaacc
      661 gtctcgtgtg atttgatagc aagggagctt gcgtgtacca ccgttcggtt gatttaccga
      721 aatcgatgtc tccgcaaaat tgcgggcaca aaactcaggg gaacaatcca gagtgcggat
      781 ctttttcagc acatcgtcgt gacgcttgga gaagaagttg gcaacagcca aagaagtggt
      841 aacggcctgg ccgttgtcaa tggtgatttc aggttgagtg agggcttgga tcgtagccat
      901 gatggcagcc tccgttgaca gtgaaaaact tccaccaccg gaaacgccaa tttcactggt
      961 ggtgaactgg acggggttgg cgtaaccggc gtcaacggag accggcgcac ctttcggtgc
     1021 ccccgcccag cccaccataa tctggatgtg agcaaatgcg gacgataaaa aagacgctgg
     1081 cgcgtcatac atcgccgttg acaatttcag gacgccaatc ccggcacccg ctttataagg
     1141 tgcctgaaca gtgtaacgtc ccggaattgc agaatcaata tgctggtggt ccttcacact
     1201 caacaaaatc acgcctgaat ttccacaaag gactaaagca ctcatgcggg tagtctttgc
     1261 gaagatagat aacgcgctgt gtttctggct cccaacgaat aacatgaaca taaagtcctc
     1321 ttccgtcacg aaaccagcgg ttaagttcct gcacaactcg ccccccacag tcaggtaaag
     1381 ttctctgtgg ttacttacag ccaggtgatt tggtaatctg cattcatgcc gtaacaacag
     1441 gtgtgcagcg acactgacca ccagctgttg cgacaaacgg ttatttgccg ttaaactgtt
     1501 catgcgttag tttctccaca gacacaaaac gccacgacgc ccggagctgc acactcgcgg
     1561 gcgttactct tttctggagc gcagaagatt ttgtagacca gtgctgcatg ctcctggagc
     1621 ttcgaaattg acagatacaa ctcatcatta attgctgtct gctcgtgtgg ctccactacc
     1681 ccatcttcga ttgccgaacg aatctgcttt gagtaactcc cgatctgttc gatgacttcc
     1741 agcaggcgct ggtttatatc ggcgttctct acttcctcaa tttctggaag tgatacaaac
     1801 accccaccag cagactgtgc gacagcatcc gcaatgtggt gagtaccagc cgcacgctgt
     1861 aaaaccattg cccatcccaa cggaaaaaac tgatcgccat cggcacgaag gcggttgaat
     1921 aaagcgttct ctgttacatc cagccactca gcagcttcag cgtaaccccc cggcaacgcc
     1981 gcgatagttt ttctgacagc tttcacgtac cactcaggct gtttttccac tttccagtga
     2041 tgcttaccca cggcttacct cctgttcctg tggttttaac tcattccggt tttgactaga
     2101 ttgaaagcgt gcaggataga gaatctgcat ttcgctgatt tctcccttaa aaaaattggc
     2161 cagacgttct gcaagctcga tagatggaat ttgttccagt ctttcaatac gactcagcgt
     2221 cgctggattg acctgaacgc ccgcagcaac atgctgcaaa gtaaatccgt gcgccttacg
     2281 cacattccgt aatggtgatt gcatataacc tccacatatt gcgtgatgag catattattt
     2341 cacgcaaata ttttgcgcaa gttgatttgc ttaacgcgca ataaagaaat gtaataaacg
     2401 catgaacata ggaaatcgag tcagacaact tcgccaggcg aagaacatga aaatcgccga
     2461 tctcgctgaa gcaataggag tggatgcggc gaatatctcg cgcctcgaaa caggtaagca
     2521 gaaacaattc actgaacaag ccctgagtaa tattgccagg agcttaggtg ttgatattgc
     2581 tgatctcttt acctcagact tcaaaagtaa tactgtatgt aaaaacagta ttagtgagga
     2641 tattgcgcag gtgaaggatg tattccgtat tgaaatgctg gatatcagtg ccagtgcggg
     2701 aaatggcctt atccagggcg gagatgtcat tgatgtgatt catgccattg aatacagaac
     2761 tgataatgct gtatcgatgt ttggcggacg gccagcaaat cacattaaag ttatcaacgt
     2821 tcgtggggac agtatgtgtc caaccattga gccaggagat ctcatcttcg ttgatgtcag
     2881 tatcaatcag tttgatggag atggtatcta tgtatttggt tttgatgata aaatttatgt
     2941 caaacgactg caaatgatac ctgacaaact actggtgatt tctgataacc agatttatcg
     3001 tgaatgggga attaccagcg aaaatgaaca ccggtttatg gtctttggaa aggtcttaat
     3061 cagccagtca caaaccctta agcgacacaa ttaaccctta cctcctcatc aattagccac
     3121 ccgaaggtgg cttttcatta cccatcaaat tgcatatctc gcaacaaaaa tacttgcata
     3181 atgcgcaact tcattttatc tttctttcca gaccaacaaa caaggtacta acaaaatttg
     3241 gttgtaacac ggcgtatggc acatgcgtcg ttagcggtct ggggacgtta aaggggacaa
     3301 tccactcctt gctcgggcaa acaaaccagg tagccggaat gtgcaagtca atgatgatgc
     3361 tgataagacg cctaaccagc gtggcgattc ggtttgacgc ctgggaagag accagggggc
     3421 aactatgagg gcatttatgg aaccgcgaca aagtgtggtg ccgtaactgg ctaagtgctc
     3481 tcagcgttgt ggtgaatgcg caggctgatg cgcgaaagac attgcagcta ttgcggaaaa
     3541 gagctgttcg gcggggcaat taaatgcccg tgagagtctg aaataaccgc aagccggaga
     3601 tcatcaccga tcaccacaac agccactgct ttggcggtac cagtttgtac acttgcttcc
     3661 ggctggtacc gctcttttta caaaacagag aagagcatca ccggacgacg ggctcataac
     3721 ccaatccatc cgggcggctg ccaccgcagg tgttcttctc tgttttgtgg agaaaccaac
     3781 cgaccttgca gggtcgatat gatgaggagc agcaaaatgg ctagcgaacg cagtactgat
     3841 gtgcaggcat ttatcgggga gctggacggc ggcgtatttg aaaccaaaat cggcgcagtt
     3901 ctcagtgaag tcgcttccgg tgtgatgaac acgaaaacca aaggtaaggt ctcactcaac
     3961 ctggaaatcg aaccatttga tgagaaccgt gtgaaaatca aacacaaact ctcatatgtt
     4021 cgcccgacta accgcgggaa aatttccgaa gaagacacca ccgaaacgcc gatgtatgtc
     4081 aatcgcggtg gtcgtctgac tattctgcag gaagaccagg gacaattact gactcttgcc
     4141 ggtgaacctg acggaaaact ccgcgcagca ggtcgttaat atcgttctta ataaactgat
     4201 tttttatc
//
LOCUS       DAZFAH010000283         4200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFAH010000283 DAZFAH010000000
VERSION     DAZFAH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.285"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..1861
                     /locus_tag="ACHJWB_004581"
     CDS             41..1861
                     /locus_tag="ACHJWB_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4316070.1"
                     /translation="MKMIFTGKVSGEKTVLTAGGRHTVKAQPGEQYGLIDEVTGLVPD
                     GVEADRSGDDLILRKKEEDTEVRIEGFWEECQPGETQCTAVFNIVGEDGQVTEAVLTQ
                     DGPVLDDITAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGSKHRHENDDSD
                     TTAPSSPALKAEDDGSVSVVLPGDANKGDTVEITFEDEKGGKHTVTLEKGDNGWTSSD
                     PTLIPDSNGDKVTIPADNVKDNSEVTAIAKDPSGNESDPSTVTSKTDGVADAPVLSIP
                     EVADGYANADELKDGLQAEVTLPAGTAEGAVITLTVTRPDKTTENVTHTVTKDEVAAG
                     KVSMDIPKDAVIDGQNSISVSLTQGSNPAKPGNVVDFTADTQIPGDTDGDGATDATPV
                     VAIPEAADGVNAEELKDGVQTEVTVPKGSAAGDTLTLTVTKPDGTTDTVEHTLTADEV
                     TAGKAAVTIPADKVTADGQYSVTAEITDPAGNTSGQGQPTDFTVDTQIPGDTDGDGVV
                     DATPVVTIPEAANGVNAEELKDGVQTEVTVPKGSAAGDTLTLTVTKPDGTTDTVEHTL
                     TADEVTAGKADVTIPADKVTADGQYSVTAEITDPAGNTSG"
     gene            2297..>4200
                     /locus_tag="ACHJWB_004582"
     CDS             2297..>4200
                     /locus_tag="ACHJWB_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316071.1"
                     /translation="MDVPATIEDKDDSTGLVYTVSLNHVTTTDVTVTVTLGSGAGHSD
                     AADYSDIGGAQHNGKIGLHGDTGKVTYDGATTVTIVIPAGSKSVSFIVDPTLEANQDA
                     FNAEGMEKVVATITGTSDNVTAATDIVDNAGASATGVIYDGNAISLRNLDGDFTLKYS
                     LSSSVAEKGDFGYTIGANSGENDPMVTTDYNDTVYVGYYQSGKETTSYSNVANSQDNG
                     PDGTKTDGNQSITTVDLGAGDDLMVIRGNMLANTRVYTGEGNDTFTMDGMNTALRVMY
                     AGSYIFTESGDDIVTIKRTGVTNAGQIYLGSGSDTFIQGDATDNNDTTLSGLLDLGSG
                     TKDISNMPKEYLSVYQDGSNLSLGNDNNIDTATDVNTVTIYGSVSGEILGGYGSDNIT
                     VTKNLTGNISVGDNADTLTAGWIYGGATVSMGDGNDTVTVTDGAYNTTISLGAGDDVF
                     DSTGATLGSAATTIDGGEGNDTIKIGTISNGNITIDAGAGDDIVVLTKDYDTKPVGNQ
                     GSINGGEGSDTLVLAGNISVNLATGKNEGIAGFEKVDMTVGSDLKAGNTAQLVKLTAS
                     DVLGMNDNSTLYISGGANDKVDLGADGAGSLGTFTATATTVKATALDGIEHTYTLYSS
                     VSGANVYIDN"
BASE COUNT         1175 a          815 c         1190 g         1020 t
ORIGIN      
        1 tattgataat cgcaggtttt aattagccta aggattttat atgaaaatga tttttaccgg
       61 taaggtcagc ggagaaaaaa ccgtgctgac tgccggaggt cgtcatacag ttaaagcaca
      121 gcccggggaa cagtatggac ttatcgatga ggtgaccggc ctggtgcctg atggtgttga
      181 ggcggatcgt tccggtgatg atttaattct gcgtaaaaag gaagaagata ctgaggtccg
      241 gatagaggga ttctgggaag aatgccagcc cggggaaaca cagtgtacgg cggtatttaa
      301 tattgttggg gaagatggac aggtcaccga agctgtactg actcaggatg gtccggtgct
      361 tgatgatatt actgccggac aatcgggaac cttgtcagat gatgatcgtg gcggttttat
      421 atggttgggc ggtctggcat ttggtggggg actggctgct atggctttag ctgcaggtgg
      481 cggtggaagc aaacatagac atgaaaatga tgactctgat actacagccc catcgtcacc
      541 agcactgaag gcagaggatg acggttctgt ttctgttgtc ttgccgggtg atgccaataa
      601 aggtgatacc gttgaaatca cctttgaaga tgaaaaaggt ggtaagcaca ccgtgacgct
      661 ggagaaaggg gataacggct ggacatccag tgacccgacg ctgatcccgg acagcaatgg
      721 cgataaagtg accattccgg cagataatgt gaaggataac agtgaagtca ccgccattgc
      781 caaagatccg agcggtaatg aaagcgatcc ttcaaccgtg acgtcaaaaa ctgatggtgt
      841 ggcagatgcc ccggtcctga gcattcctga agtggctgac ggttatgcca atgccgatga
      901 gctgaaggat ggtttgcagg cagaggtcac gttgccggca ggcacggcag aaggtgcggt
      961 tattacactg actgtgaccc gtccggataa aaccacggag aacgtgacgc ataccgtgac
     1021 taaagatgaa gtggctgccg gtaaagtatc catggatatt ccgaaggatg cggtgattga
     1081 cgggcagaac agcatcagtg tcagtctgac tcagggcagt aacccggcga agcccgggaa
     1141 tgtggttgat tttactgccg atacccagat cccgggggat accgatggtg atggtgcaac
     1201 agatgcaaca ccggtagtag ccattccgga agcagcagac ggcgtgaatg ccgaagagct
     1261 gaaggacggt gtgcagaccg aagtcacggt accgaaaggc agtgctgccg gtgataccct
     1321 gacgctgacg gtcactaaac cggacggcac gacggacacg gtggagcaca cgctgaccgc
     1381 agatgaagtg actgcaggta aagcggctgt gaccattccg gcggataaag tgacggcaga
     1441 tggccagtat tctgtaactg cggaaatcac agacccggca ggtaacacga gtggccaggg
     1501 ccagccgaca gactttactg tcgatactca gatcccgggc gatactgacg gtgacggtgt
     1561 ggtggatgca acaccggtag tgaccattcc ggaagcagca aacggcgtga atgccgaaga
     1621 gctgaaggac ggtgtgcaga ccgaagtcac ggtaccgaaa ggcagtgctg ccggtgatac
     1681 cctgacgctg acggtcacta aaccggacgg cacgacggac acagtggagc acacgctgac
     1741 cgcagatgaa gtgactgcag gtaaagcaga tgtgaccatt cctgcagata aagtgacggc
     1801 agatggccag tattctgtaa ctgcggaaat cacagacccg gcaggcaaca ccagtggcta
     1861 ggggcagccg gcagacttcg cagtggatac agtggcgccg tcggcaccgg tattaaaagc
     1921 agaggatgat ggttctgttt ctgttgagct gccgggtgat gccaataaag gcgatactgt
     1981 ggatgtcacc tttgaagatg aaaaaggtgg taagcacacc gtgacgctgg agaaagggga
     2041 taacggctgg acatccagtg atccgacgct gatcccggac agcactggcg ataaagcgac
     2101 cattccggca gataacgtga aggataacag tgaagttacc gggggtgcca aagatccgag
     2161 cggtaatgaa agtgacccgt caaccgtaac ctcaaaaact gatgtgttac caacagtgag
     2221 tattagcgtt gagacaacat caacagatgt caatggtgat ggttttactg gtatagctag
     2281 tgtcaatggg accgtaatgg atgttcccgc aactattgaa gataaggatg attccactgg
     2341 actggtttat acggtttctc tgaatcatgt cactacaaca gatgtaacag ttacagttac
     2401 attgggtagc ggagctgggc attctgatgc tgctgactat tctgatattg gtggagcaca
     2461 gcataacggg aaaataggcc ttcatggtga tactggaaaa gtaacctatg atggtgcaac
     2521 aaccgtaaca attgttattc ctgccggttc aaaaagtgtt tcatttatag ttgatccaac
     2581 gcttgaggct aaccaagatg cctttaatgc cgagggtatg gaaaaagttg ttgcgaccat
     2641 tacgggaaca tctgataacg ttacggcagc tactgacatc gttgataatg cgggagcatc
     2701 cgcaacaggt gttatttatg atggtaatgc catttcatta cgtaatctgg acggtgattt
     2761 tacactgaaa tattcactat catcttctgt cgccgaaaaa ggggactttg ggtataccat
     2821 tggcgcgaat agcggtgaaa atgatccaat ggtaacaaca gattacaatg atacggttta
     2881 tgttggttat taccagagtg gtaaagaaac aacatcgtat agtaatgttg ctaactcaca
     2941 ggataatggg ccagacggaa caaaaaccga tggcaatcaa agcattacga ctgttgattt
     3001 aggtgctggt gatgatctaa tggtcattcg tggtaacatg ctggctaata caagagtata
     3061 cactggtgag ggtaacgata cttttactat ggatggtatg aatactgcat tacgtgttat
     3121 gtatgcgggt agttatatct ttactgagtc tggcgatgat attgttacga tcaaacgtac
     3181 tggtgtaaca aatgccggac agatttatct tggttcaggt agtgatacat ttatccaggg
     3241 ggatgctact gacaataacg atacaacatt atctggttta ttggatcttg gtagtggtac
     3301 aaaagatata tctaatatgc ctaaggaata tctttctgtt tatcaggatg gcagtaattt
     3361 aagcttgggt aacgacaaca atattgacac agccacagat gttaataccg tcactattta
     3421 tggctctgta tctggtgaaa ttttaggcgg atacggtagt gataatatta cggtaactaa
     3481 aaatctgacc ggtaatattt ctgttggtga taatgccgat accttaactg ctgggtggat
     3541 ttatggtggt gctactgttt ctatggggga cggcaatgat actgttaccg tcactgatgg
     3601 tgcatataac acgactattt cactgggggc gggagatgat gtatttgatt ccactggtgc
     3661 aactttaggt tcagctgcga cgaccattga tggtggggag gggaatgaca caatcaaaat
     3721 aggtactatt tcaaatggta atataaccat tgatgcgggt gccggtgatg atatcgttgt
     3781 tctaacaaaa gactatgata ccaaacctgt tggtaatcaa ggttccatta acggtggaga
     3841 aggtagcgat actctggttc tggcaggtaa tatttctgtc aatctggcta cagggaagaa
     3901 tgaaggtatt gccggcttcg agaaagtgga tatgactgtt ggcagcgatc tgaaagctgg
     3961 caatacagct cagttagtca agttaacggc atcggatgta ttgggtatga atgacaattc
     4021 aacgctttat atttccggtg gtgccaatga caaagttgat ttgggtgctg acggtgcggg
     4081 aagtctgggt acatttacgg caacggcaac aactgttaaa gcaactgcgt tagatggtat
     4141 tgagcatact tatacgcttt acagcagtgt aagtggcgca aatgtgtata ttgataataa
//
LOCUS       DAZFAH010000284         4161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFAH010000284 DAZFAH010000000
VERSION     DAZFAH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.286"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..688
                     /locus_tag="ACHJWB_004583"
                     /pseudo
     CDS             1..688
                     /locus_tag="ACHJWB_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            685..768
                     /locus_tag="ACHJWB_004584"
                     /pseudo
     CDS             685..768
                     /locus_tag="ACHJWB_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            783..1436
                     /locus_tag="ACHJWB_004585"
     CDS             783..1436
                     /locus_tag="ACHJWB_004585"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4316072.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            1433..1822
                     /locus_tag="ACHJWB_004586"
     CDS             1433..1822
                     /locus_tag="ACHJWB_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4316073.1"
                     /translation="MKLILPFPPSVNTYWRHPNKVAFAGKSLISEAGRKFQSAACAAI
                     IEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            1842..2651
                     /locus_tag="ACHJWB_004587"
     CDS             1842..2651
                     /locus_tag="ACHJWB_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4316074.1"
                     /translation="MNNLMVIDGIEVRRDVYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSTPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            2659..3648
                     /locus_tag="ACHJWB_004588"
     CDS             2659..3648
                     /locus_tag="ACHJWB_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4316075.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWLHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            3666..4007
                     /locus_tag="ACHJWB_004589"
     CDS             3666..4007
                     /locus_tag="ACHJWB_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4316076.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
BASE COUNT         1033 a          946 c         1180 g         1002 t
ORIGIN      
        1 ttgctgacca gtgcgagatt agcaaacgtt ctgtgatgaa tcatattgcg gctctttgtg
       61 attccggact ggtaaaaaaa gtcacccgga aaggtgaaaa aggtaactca agtaatatct
      121 atctccttca tcttgatggt gcaggagatt cactaggggg tagtgcaaat aattcactat
      181 ctggtgcagc aaattcacca ggtagtgcag gagttgcacc agggggtggt gcaggagatt
      241 cacccagaac cagtcactct tttgaaccag tcaaagaacc agtcaatgaa ccaatagctg
      301 ttggtgcatc tgctgatgag tctgtgcgag ttcgttcaaa ccgaccggaa tactctccgg
      361 agtttgagca ggcatggctg gcatacccca aacgtgctgg tggcaattca aaatctgcag
      421 ccttcaaagc ctggaaagcc cgtttgaatg agggggtaaa acccgaaacc atgctggaag
      481 gtgtgaaacg ctacgcgggc tgggtatctg cgatgggtaa cagcggcaca caatttgtga
      541 aacaggcacg ttctttggcc cggatcgtca tttcgaagag tcctgggaag ttcctgcggt
      601 atctgcagcc agacgcgagg acccgtactt caaagccagt tacgacaacg tggactacag
      661 ccagatcccg gcaggattca gggggtgatc atgagtctgt taaatgacgt tcagaaattc
      721 attgaagccc atccgggctg tacttccgga gacattgcgg atgcttttta cgtgggggct
      781 taatgagtaa taaatattgc caggcgctgg tagaactgcg gaacaaacca gcccatgaac
      841 tgaaggaagt gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacacct
      901 tgtttggtcc gtttgttctg gatctgttca ctgacggtga taacgccaaa tgtgccgcgt
      961 attacacggc ggaagacaac gcgctggcgc atgactggtc agaacgtctt gcggagctta
     1021 aaggtgctgc ctttggcaat cccccgtaca gccgcgccag tcagcatgag gggcaataca
     1081 tcaccggcat gcgttacatc atgaagcatg ccagtgccat gcgtgataaa ggcgggcgct
     1141 atgttttcct gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcagatcata
     1201 ttgcttttat tcgcgggcgt attggttttg aactgcctgc ctggtttatc ccgaaagacg
     1261 aaaagcaggt gccaacaggt gctttcttcg ctggtgctat tgctgttttc gacaagacct
     1321 ggaagggacc ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcat
     1381 ttctggcgca ggttcgccag caggcggaaa aactggtcag ggagatggtg gcatgaagct
     1441 aatactgcct tttccgccca gcgtgaacac gtactggcga caccccaaca aagtggcatt
     1501 tgctggtaag agcctgataa gcgaggcggg gcgaaaattt cagagcgcgg cgtgtgccgc
     1561 catcattgag cagttacgtc gtctgccgaa accaacgtcg gcacctgctt cagtggagat
     1621 cgtgttgttt cctccggata acaggatccg cgatctggac aactataaca aggcgctgtt
     1681 tgacgccctg acccacgcgg gtgtgtggga agacgacagt caggtgaaaa gaatgctggt
     1741 ggagtgggga ccggttatcc cgaaagggaa ggtcgagatc actatcagta agtacgagaa
     1801 aacggcgggt gcagccgcct gatcaagagg agaaacgaag tataaataat ctgatggtca
     1861 ttgatggtat tgaagttcgt cgtgatgttt atgggcgtta cagcctgaac gatctacatc
     1921 gcgcagcagt agcatctggt gcaaatgcca gaaccaagga gccaggaaag tttctttcca
     1981 gccaacaaac tgttgaactt gttcatgaat tgaccaacac ccagaatttg ggtgttgacc
     2041 cggtgagtgt gattcatggg ggaaatgaac ggggaactta tgtctgcaag gaactggtgt
     2101 atgcctatgc aatgtggatc agcccgtcat tccatctgaa ggtgatccgt actttcgaca
     2161 tggtaaccag cacaccggaa aaattatccg gacaggctgc tgacaagatg caggctggtg
     2221 tgattctgct ggactttatg cgtcgggaat taaacctgtc taactcatct gtgcttggtg
     2281 cctgtcagaa actccaggag gctgttggct taccgaatct ggcaccgcgc tatgccattg
     2341 atgctcctgc tgatgcacac gatggctcaa gtcgcccgac actgtcactg agtgcactgc
     2401 tgaaacagta tggtatccgc ctgacggcta atcaggcata tcaccagatg gtgaagctgg
     2461 ggatcgtcga gcagcgcgaa cgatacagcc gtaccgcgat taacaacatc aaaaaattct
     2521 ggtcgctgac agcgaaaggc tgcatgttcg gcaagaacat caccagtccc gcaaatccgc
     2581 gcgagacgca gccgcatttc ttcgaatccc gattccctga gctgttaaag ctgctcgata
     2641 ccgttcattg aggtgaccgt gagagcacta ctgacccctg aaattgcccc gcgtatgggg
     2701 atcgtattgt tcaggccagg ttcagagctg atgcccctgt ttatgcaggg gcgtgtcctg
     2761 ctggagcctg agccggaacg ttattcatct ttcgctagtg gtgccgttcc ggcggcatca
     2821 caaccgctgg cggatgatcc tgccgttcgg gccgtgttcc gcaatgaggc agtgatccgt
     2881 cgtgctggtg gcgtggaatg tcttgaaagc tggttacttc gtgaaaaagg ctgccagtgg
     2941 cttcattccg actggcacag cgagaacatg accacaatgc gacacgctcc gggtgcaatc
     3001 cgtctgtgct ggcactgcga taaccagctg cgcgatcagt tcacggaacg gctggaatca
     3061 atggcaacgg ataactgtgc ccgctgggtg ttgtctgttg tgcgtcggga tctcggtttt
     3121 gatgacagtc acgttgtgac aatgccggaa ctgtgctggt ggctgattcg taatgacctg
     3181 gcggatgcct taccggaaag tgcagcccgt aaggcactga gattaccgaa gcctgttgtg
     3241 ccgtctgtca cccgggaaag tgaccttgtg ccttcggttc ctgccaccag catcatccag
     3301 gataaagcga aaaaggtgct ggtgctgaaa gtggatccgg agtcgccgga gtcttttatg
     3361 ttacgcccca aacgtcgccg ctgggttaat gaaaagtaca cgcgctgggt taagacgcag
     3421 ccgtgcgcat gttgtggaaa gccagctgat gatccccacc acctgatagg ccacggtcag
     3481 ggtggaatgg gtacaaaagc gcatgatctc tttgtgttgc ctttgtgcag aaagcatcac
     3541 gacgagctgc atgcggatac cgtgacattt gaagagaagt atggctccca gctggagctg
     3601 atatttcgtt ttatcgatcg tgcactggca attggcgtgc tggcctgatt ttgtggagaa
     3661 agttgatgcg tgatatgtat gaagtattgg accgctgggg tgcatgggct gcagcagata
     3721 acagtggtgt ggactggcag cctgttgctg cagggtttaa aggtttacta cctcatggaa
     3781 agaaaacacg ccaacaatgc gatgatgatg aaggaatcat gattgacagc tgtgttgcgc
     3841 gattgcggaa atataagccc gaagagtatg agttggttat tgctcatttt gttatcggca
     3901 tctcactaag aactattgca aagaagcaga agtgttctga tgggacaata agaaaagagt
     3961 tgcaaacagc tcttggtttt ctggatggtg tgctttcaat gttgtaatat tagggggaat
     4021 tacccccctt tttttctctg ttgctttaat aaaattttaa tattttgtct gattatgatg
     4081 aggcaatgta ataaaagaaa taccgttagt attgcaagcc atacgcaaaa taagcatgca
     4141 tataaattag ttgaaagtcc a
//
LOCUS       DAZFAH010000285         4152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFAH010000285 DAZFAH010000000
VERSION     DAZFAH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.287"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1949)
                     /gene="speA"
                     /locus_tag="ACHJWB_004590"
     CDS             complement(<1..1949)
                     /gene="speA"
                     /locus_tag="ACHJWB_004590"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4316077.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGL"
     gene            complement(1958..2089)
                     /gene="yqgB"
                     /locus_tag="ACHJWB_004591"
     CDS             complement(1958..2089)
                     /gene="yqgB"
                     /locus_tag="ACHJWB_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4316078.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2183..2326)
                     /gene="yqgG"
                     /locus_tag="ACHJWB_004592"
     CDS             complement(2183..2326)
                     /gene="yqgG"
                     /locus_tag="ACHJWB_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4316079.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2437..2688)
                     /gene="yqgD"
                     /locus_tag="ACHJWB_004593"
     CDS             complement(2437..2688)
                     /gene="yqgD"
                     /locus_tag="ACHJWB_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4316080.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2744..3898
                     /gene="metK"
                     /locus_tag="ACHJWB_004594"
     CDS             2744..3898
                     /gene="metK"
                     /locus_tag="ACHJWB_004594"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4316081.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1040 a         1124 c         1008 g          980 t
ORIGIN      
        1 tacaaacctg cctcgaactc ttcaaggaac tgttgttgca gttcagcatc aagatcggtt
       61 ttcttcactt gatcgcggaa ctgggttaac agcgttttcg gatcgagctg tacatattgc
      121 agcatgtccg ccacggtatc gccttcgtca gacagttcta cttctacgct accgtcaggg
      181 aacacgaaca cgtcaaccgc ttcggtatca ccgaacaggt tgtgcatatt gccaaggatc
      241 tcctgatatg cgccgaccat aaagaaaccg agcatcggcg gattctctgg atcgtactcc
      301 ggcattggca ttgtcgtggc aataccatca ccatcaatat agtggtcgat agcaccgtca
      361 gagtcacagg taatatccag cagcacagcg cggcgttccg gcacttgatc cagcccttcc
      421 agcggcagaa ccgggaacaa ctggtcgatc ccccacgcat ccggcatcga ctggaacagc
      481 gagaagttga cgtacatttt gtctgccata cgttcctgca gctcgtcgat aatcggacga
      541 tgagcacggt tttgcggatc cagttgcttc tgcacttcat ggcacatgct cagatagagt
      601 tgttctgccc atgcacgttc ttgcaggcta aaggtgccgg aagagtagcc gatatgaatg
      661 tcgtgcagat ccatctggct gtcgtgtaac cattcacgca aagaacggcg agttcccggt
      721 tcgtgcatct cctgccaggt ttcccacatg ctttgcagcg cgcgcggcgc atcttctgcg
      781 ggcgcggtcg gcaccgtata ttcgttacgt tccacgccga tgatattcga taccagcacg
      841 gtgtgatgcg cagttaccgc acgacccgat tcggtgatta ccgtcggatg cggcagacca
      901 ttttcttcac acgcatcacc aatcgcccag atgatgttgt tggcgtattc attgaggcca
      961 tagtttacgg agcagtcaga ctgcgaacgg gtgccttcat agtccacgcc cagaccgccg
     1021 ccgacgtcga agcactgaat attgacgccc agcttgtgca gttccacata gaaacgcgcg
     1081 gattcacgaa cgcctgtcgc gatatcgcga atattcgcca tctgcgaacc gaggtggaag
     1141 tgcagtagtt gcaggctgtc gagacgcccg gcttcacgca gggtttcaac cagttgcagt
     1201 acctgagtcg cagccaggcc gaacttcgat ttttccccgc cggaggactg ccatttaccc
     1261 gagccctgcg aggccagacg tgcacgcacg cccagacgag gaacgacatt cagacgttct
     1321 gcttcatcca gcacaatggc gatttctgac atcttctcaa tgaccagata gaccttgtgc
     1381 cccatcttct cgccaattaa tgccaggcgg atatattcgc ggtctttata accgttgcag
     1441 acgatgacgc tacgggtcat gccagcatgt gccagcaccg ccatcaactc tgctttggaa
     1501 ccggcttcca gacccagcgg ttcgcccgaa tgaatcaggg actcgatcac gcggcggtgc
     1561 tggttaactt tgatcggata aacaaggaag taatcgccgt tatagccgta ggattccctc
     1621 gcacgtttga acgcggcgtt aatggaacgc aaacggtgct gcaggatctg tgggaaacag
     1681 aacagtgcag gcagacgctg accctgtgct tcacgagttt tcactaactg cgcgagatcg
     1741 acgcgagctt ccgggacgtc cgggtccggg cacacgctga tgtggcccag ctcgttaacg
     1801 tcatagtagt tattgcccca ccaggcaata ttgtaagtac gcagcatctt gctggcttcc
     1861 tgggagctca ttgcaacctc ctgcatggag cgtagtacac cgtgttcgcc cgctgacgaa
     1921 ggcaaaccca tagacatgtc gtcagacata gcgaacctca aattatttta ttaagtgtaa
     1981 aacagttaac gactatcgca gcctggaact gcgataacaa cccataagca catggatttt
     2041 ccagcagtgt atgctgacgc tccgactgcg caaccggttt ctttttcata acattattaa
     2101 gcacataacc gaacgtaagt gtgacagttc ggcgaaacca cgagaaaact cttgttttta
     2161 caagagcgcc cttgttcagt cctcagtaac tgtaaccagc tcttgaatcc tgagaagcgc
     2221 cgagatgggt ataacatcgg caggtatgca aagcagagat gcagagtgcg gggaacgaat
     2281 cttcaccaga acggtgagac aggttaagca gcagacaacg gttcattatt tcgtatcacc
     2341 tccacggccg cctgttaaga cgaacccaca agccaaaact ctgatttcaa cccggctgga
     2401 agtggcaaca cgaaagaaac gtcgtgtgct ttttatttaa gccgcgcgcc gcgttttata
     2461 ccccacaatg gcagaaaatt gcaaaagata aatacgcaga atgccggcat tgtcaggaaa
     2521 aatttccagc cacgttttta acagaatgag acacgattca aaaaaaagtg gaaatcgggt
     2581 gaagaattga cctaaaatag ccatccagat gttaatccat ccataccgat taacactcag
     2641 actgccagtg tttttaactt gcagagtcgt ggtaggatcc gctaccacag aaaatccaca
     2701 caacagtttg agctaaccaa attctcttta ggtgatatta aatatggcaa aacacctttt
     2761 tacgtccgag tccgtctctg aagggcatcc tgacaaaatt gctgaccaaa tctctgatgc
     2821 cgttttagac gcgatcctcg aacaggatcc gaaagcacgc gttgcttgcg aaacctacgt
     2881 aaaaaccggc atggttttag ttggcggcga aatcaccacc agcgcctggg tagacatcga
     2941 agagatcacc cgtaacaccg ttcgcgaaat tggctatgtg cattccgaca tgggctttga
     3001 cgctaactcc tgtgcggttc tgagcgctat cggcaaacag tctcctgaca tcaaccaggg
     3061 cgttgaccgt gccgatccgc tggaacaggg cgcgggtgac cagggtctga tgtttggcta
     3121 cgcaactaat gaaaccgacg tgctgatgcc agcacctatc acctatgcac accgtctggt
     3181 acagcgtcag gctgaagtgc gtaaaaacgg cactctgccg tggctgcgcc cggacgcgaa
     3241 aagccaggtg actttccagt atgacgacgg caaaatcgtt ggtattgatg ctgtcgtgct
     3301 ttccactcag cactctgaag agatcgacca gaaatcgctg caagaagcgg taatggaaga
     3361 gatcatcaag ccaattctgc ccgctgaatg gctgacttct gccaccaaat tcttcatcaa
     3421 cccgaccggt cgttttgtta tcggtggccc gatgggtgac tgcggtctga ctggtcgtaa
     3481 aattatcgtt gatacctacg gcggcatggc acgtcacggt ggcggagcat tctctggtaa
     3541 agatccatca aaagtggacc gttccgcagc ctacgcagca cgttatgtcg cgaaaaacat
     3601 cgttgctgct ggcctggccg atcgttgtga aattcaggtt tcctacgcaa tcggcgtggc
     3661 tgaaccgacc tccatcatgg tagaaacttt cggtactgag aaagtgcctt ctgaacaact
     3721 gactctgctg gtacgtgagt tcttcgacct gcgcccatac ggtctgattc agatgctgga
     3781 tctgctgcac ccgatctaca aagaaaccgc agcatacggt cactttggtc gtgaacattt
     3841 cccgtgggaa aaaaccgaca aagcgcagct gctgcgcgat gctgccggtc tgaagtaatc
     3901 tttcttcacc tgctttcaaa ggccagcctc gcgctggcct ttttctttta cctcaccgcg
     3961 gtggataggc attcacgccg catccggcaa aaagctgccc gcacaataac atcattcttc
     4021 ctcaacacgt tttatcgcaa attatcatca ctactgaaac cgattacacc aataacacca
     4081 gccaaatatt tgtaatattt tcatattatt attcagtttt cacagttgtt acatttcttt
     4141 tcagtaaagt ct
//
LOCUS       DAZFAH010000286         4136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFAH010000286 DAZFAH010000000
VERSION     DAZFAH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.288"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..926
                     /gene="ybeF"
                     /locus_tag="ACHJWB_004595"
     CDS             <1..926
                     /gene="ybeF"
                     /locus_tag="ACHJWB_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4316082.1"
                     /translation="LSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLN
                     LTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGS
                     YDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIID
                     NMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLR
                     QRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLN
                     EEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1135..2100
                     /gene="lipA"
                     /locus_tag="ACHJWB_004596"
     CDS             1135..2100
                     /gene="lipA"
                     /locus_tag="ACHJWB_004596"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4316083.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHJWB_004597"
     CDS             complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHJWB_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4316084.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2533..3321)
                     /locus_tag="ACHJWB_004598"
     CDS             complement(2533..3321)
                     /locus_tag="ACHJWB_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4316085.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIVPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3414..3797
                     /gene="crcB"
                     /locus_tag="ACHJWB_004599"
     CDS             3414..3797
                     /gene="crcB"
                     /locus_tag="ACHJWB_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4316086.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="ACHJWB_004600"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="ACHJWB_004600"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4316087.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1072 a         1067 c          948 g         1049 t
ORIGIN      
        1 gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg
       61 atcttaacct tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag
      121 cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta
      181 tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga
      241 tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgca ctggatatcg
      301 aaggaagcta tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc
      361 tggtccttcc tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca
      421 acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg
      481 ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg
      541 tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg
      601 acaacgctgc gcatgtactc ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga
      661 gagttcaaga gatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa
      721 tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc
      781 tgtttagtcg ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa
      841 tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg cacggggtaa
      901 tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa
      961 cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc
     1021 aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca
     1081 atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt
     1141 aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc
     1201 ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa
     1261 atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat
     1321 ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac
     1381 ggcacagcaa cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgac
     1441 gttgcccacg gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc
     1501 attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc
     1561 gatggcggtg cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa
     1621 atcaaaattg aaactctggt gccggatttc cgtggccgta tggatcgtgc tctggatatt
     1681 ctgactgcaa cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac
     1741 cgtcaggtac ggcctggtgc agattacaac tggtcgctga agctgctgga acgctttaaa
     1801 gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat
     1861 gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg
     1921 gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat
     1981 gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt
     2041 ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa
     2101 ttctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt
     2161 gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct
     2221 tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg
     2281 ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag
     2341 tcccaaacag cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac
     2401 ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata
     2461 ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg
     2521 agtaaaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag
     2581 ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat
     2641 ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt
     2701 tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc
     2761 aagcaacgtt gaccagtgat gctctttgag cggcccgcga acccaggcgg caggaagtac
     2821 caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata
     2881 gcaggtcatc agaccgacct tcatcccttc cacctccagt aacggaacga tttcattacc
     2941 agcatcaaca tggcgtgatt cctgaatggc aaatgcatca tagagatgca gtttggcata
     3001 acgggcgacg atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt
     3061 tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa
     3121 acgtccgagg aattcacctt ccagcagctg tgctgattta accgatagat ctgcatcatg
     3181 atcatcgcgc gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc
     3241 ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa
     3301 ctgtcctgca gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg
     3361 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
     3421 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
     3481 gtatgcgatt taacccactg catcaggcga ttccgttggg gacgctggca gcaaacctga
     3541 ttggggcatt catcatagga atgggattcg cctggttcag caggatgacg aacattgatc
     3601 ccgtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
     3661 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggcg ttactgaacg
     3721 ttttcgtcaa ccttctgggg tcttttgcca tgaccgcact ggctttctgg ctgttttcgg
     3781 cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg
     3841 ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga
     3901 tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag
     3961 agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg
     4021 actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa
     4081 aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtc
//
LOCUS       DAZFAH010000287         4106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFAH010000287 DAZFAH010000000
VERSION     DAZFAH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.289"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..517
                     /locus_tag="ACHJWB_004601"
                     /pseudo
     CDS             1..517
                     /locus_tag="ACHJWB_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            694..942
                     /locus_tag="ACHJWB_004602"
                     /pseudo
     CDS             694..942
                     /locus_tag="ACHJWB_004602"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1448..2818)
                     /locus_tag="ACHJWB_004603"
                     /pseudo
     CDS             complement(1448..2818)
                     /locus_tag="ACHJWB_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2838..3185)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_004604"
     CDS             complement(2838..3185)
                     /gene="tnpB"
                     /locus_tag="ACHJWB_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4316088.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3182..3856)
                     /gene="tnpA"
                     /locus_tag="ACHJWB_004605"
     CDS             complement(3182..3856)
                     /gene="tnpA"
                     /locus_tag="ACHJWB_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4316089.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVAQIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          924 a          988 c         1112 g         1082 t
ORIGIN      
        1 gctgataatc atacgctatc ggctggttat gctcagagta aggtacagga ctttaagaat
       61 attaaaggag tgaatctgca atatcgatat gagtgggatt ctccggtcag tgtcgtcggg
      121 agtttttcgt atatgaaagg tgattgggcg gacagtcatc gcgatgaagc tgacgatttt
      181 tacagacatc aggctgatat taaatattat tcatttctcg caggcccagc ttatcgtctg
      241 aatgactata tcagttttta tggtttggtg gggatatctc atactaaagc gaagggagat
      301 tatgagtgga gaaacagtgt gggtgctgat gagtctgatg gatatttaag tgagtctgta
      361 tcaaaaaaaa tccactgatt ttgcttatgc agctggtgtt attattaatc cgtggggcaa
      421 tatgtctgta aatgttgggt atgagggaac taaagcagat atatatggaa aacattcggt
      481 caatggattt actgttggag ttggttatcg tttttaatca ttagacggga ctggataatt
      541 gcacttgcgt agtgaagttt actgaatgtt tgtattttaa tgaagtattc gacatattcc
      601 cttcagacgg aatatgtcga cggttatggt tttactggag catgagtatg ttatggcgaa
      661 actgataaat atatcaggtc cactgaggag agaatgacgc caggggagag gagggtggct
      721 tcacgactgg agagctttct gaatgatgat tgctttgtct ggtacgatat cccggtggga
      781 cggaaaaacc gacatccgga ttttgtcatt attgacccgg ataacgggct ggtatttctg
      841 gaggtcaagg actggactgt atccacatta cgtaaagcaa atcaggagca ggttacactg
      901 gaaaccgacg gtcttctgaa atcagaaata aatccactgg tacaggttcg tcgttatgcc
      961 tgtgatacgg ttaatgcctt acctgctgac ccgtgcttac ggcagaatga cggtcagtat
     1021 aaggggcggc tgaacctggc ctgggtgtat ggtgtggtgt ttacccgcat tacccgtcag
     1081 caactgaagg cactgacagg gaataatgag aatgcggtgg aaaagatttt tccttcagca
     1141 cagacaatct gtcaggatga aatgacgcag tctgttttac ctgaggtatt ccggcaaaaa
     1201 attgccggga tgtttaccac ggggttcaga acccgggtaa caccccggat gagggatatt
     1261 ctgcgcgccc atctgtttcc tgaagtgacc gtgaaacaga actcacaaat caaaattatg
     1321 gatattcagc aggagattct ggccagaaat attggtgacg ggcatcgtgt cattcatggt
     1381 gtggccggtt ccggaaaaac catgattctt ttgttccgtt gcctttacct ggcagaaacc
     1441 acgtccggta gttacgccgc cccagtgcaa ctacccggag ggcgttttca cacaggttat
     1501 tgtcgatctc cacccagcca ttgcggcagt attcgttcag agcgtcccat tgcttcagca
     1561 gataggtgaa cgctttcgct gtatccgagt ggcgcgacag cgtgctcatc tgcccctgga
     1621 gccactcata caacgactgc attagcggta ccgttctggc ttttctgacc gccagtcgct
     1681 cttctgccgg actgccgcgg atctcagcct cgatagcgta cagttccccg atacgacgga
     1741 gcgcttctcc cgttactgtc gttggatggc ggacatgtac atcgtggatc ttgcgtcggg
     1801 catgtgccat acaagccgct tcggttacct gaccgctttc gtaaagagca ttgtaacccg
     1861 catatgcatc ggcctgcagg atacctctgt agtccgccag atgttgctgt gggtggatgc
     1921 ctttgcggtc gggagagtat gagaaccaca ccgctgccgg catggtcgaa ccggcgttgc
     1981 gatcgtcacg aacatagacc cacagccgtc cggtacgggt tttaccctga cccggctcca
     2041 gtacatttac cggcgtgtca tcggcatgca ctttacccgg catcagcaca tagtgcttca
     2101 gttcatcata cagcgggcga agctgctctc ccatgatgtc aacccagcgc cccatcgtat
     2161 tgcggtgcag ctccacgccc tggcgggcat agatttctga ctgacggtac agcggcagat
     2221 gctcggcgaa cttagccatg attatgcggg ccagcagagc cggactggcg taactgcgct
     2281 cgatgggttt tggtggctgc ggagcctgaa ctatacagtc gcaccggctg caggcccgtt
     2341 ttgggcgaac cgtttcgatt accctgaacg cggtgttgat gatatccagt tgttcagaga
     2401 tgctttctcc cagcggtttc agtttgccgc tgcagacggg gcattcggtt tctgccgggg
     2461 agataacctg cctgtcacgg ggaagtgttg ccggaagtgc tttgcggacg ggagagtctg
     2521 atgttttcgg cgctgtctct ccggccattg aggtgagttg caactgcgcc tcaccaagca
     2581 tgttctggag ctcggttata cgcgtttctg cccgtgcgat cttcttttct atcttctcgc
     2641 ggcttttctc gctgctgcga ccgaacaaca ttctctgcag tttagcgacc agcgctctga
     2701 gtgagctgat ctcgcgggca tagccggtta tttcaccaga cagacggacg ataacagcct
     2761 gctgtgcgat cagcagggcc ttcagttgct cgatgtcgtc ggggagtgtg ttgttcattc
     2821 ccctgtttta tcacgggtta tatccggatg ccaggccgtt ctgtccgttt gggatgttgc
     2881 catgcgatcc cctccagtag catggataac tgagctggcg tcaggtgcac tttcccttcc
     2941 cgggttaccg gccagacgaa gcggccccgt tccaggcgtt tggcgaacag gcataacccg
     3001 tcacgatcgg cccacagtat tttcaccatt ttgccactgc ggtcccggaa gacgaagata
     3061 tgcccggaga acgggtcatc tttcagcgtg ttctgcacct tcgaagccag gccgttgaag
     3121 ccacaacgca tatctgtgat gccagcgatg atccagattc tggtaccggt tggcagcgtt
     3181 atcatcgggt acctcctttc atttcgcgga ttagcgcccg taacatttcc ggagtgagag
     3241 ggtcaaacag ttttaccaca cctgatttaa gatgcagctc gcaccgtggg acgtttccgg
     3301 gatcactctc agggcactca tcaggcttgt tacgccagaa gggatttgta actggtctgg
     3361 tcggctctgg cgtatcagtc agagccaccg ggacaggcat gcattcctgt atgtcatcat
     3421 cgctcagtaa gccgtcctcg tactggcttt tccatttaaa cagcaggtta tcattgatac
     3481 cgtgctctct ggcgatctgg gcaacaacag caccgggctg taatgcctgc ttagccagac
     3541 ggaccttaaa ttcacggctg tagctggctc gccgttcttt tcgccatgtg ccttcgctga
     3601 tttgaggctc tgttaattcc ttctttctgt tggcataaag gatggcgtca agctgagcta
     3661 atgaaactga atcgggcaat ggccatgcga taccggatgc aataaatcgc tgaaaaagcg
     3721 tatgtattgt ggaatgactg agacctagac gctgagcgat ggcccggatg gtcagtttat
     3781 cttcaaatct taaacgcaga gcatcaggca aataagaacg gaagcaggga atatcttttt
     3841 ttgtctggga attcatcgtt cgtgtccatc tatatagatg ggcgcgattg ttgccagaca
     3901 ggacaatttt cacaagacgt cgcagatggg gcgcttacac aacaatggcg cgagggaaag
     3961 ctgctattac cttcttcaga gagcccccag ctacttcctg tgactctcga tgcagctgcc
     4021 gaacagccag aatcgctcgc agaggacccg gaaaccctca gtatcagctg ttgtcaacga
     4081 cggatgacta ttgcaccgtt cgtgac
//
LOCUS       DAZFAH010000288         4021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFAH010000288 DAZFAH010000000
VERSION     DAZFAH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.290"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACHJWB_004606"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACHJWB_004606"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4316090.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACHJWB_004607"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACHJWB_004607"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4316091.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="ACHJWB_004608"
     tRNA            2652..2727
                     /locus_tag="ACHJWB_004608"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            2892..>4021
                     /locus_tag="ACHJWB_004609"
     CDS             2892..>4021
                     /locus_tag="ACHJWB_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4316092.1"
                     /translation="MPLNDMQIRRAKPEAKAYTLGDGQGLSLLVEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLAEARSRRDEARKIVAEGKNPSEVRKEQKLALRIQSENAFEK
                     IAREWHQMKSAKWSAGYASDIIEAFQNDIFPYVGARPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEFNPAADLSSALEVHKSNHFPFLKSDEIPDFLRA
                     LDSYTGSRLVQIATKLLMVTGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSTQALGFLNELKSMTGNYRYVFPGRNDPNKPMSEASVNQLIKRIGYAGKLTGHGFRH
                     TLSTILHENGFNTAWIEMQLAHVDKNSIRGTYNHAQYFEKRKV"
BASE COUNT         1043 a          928 c         1065 g          985 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaagtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttcttgaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggcgtca gtcgttacaa cagcgatgca ttacgccgta ttgccgggca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagcagg ctgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgtgccaa tggccttggc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgatgat gtgcgccagg tgtgtaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cagcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg
     1741 ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc
     2161 agttgcaggc aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccatct ccacttcttc aaacatccgt attaatccat
     2761 aatcatactg ttttataaaa gattttttgt tctttttgtc catgttagtc cataaccttc
     2821 cagtggaatc cagtactaaa tgtgtatagg attatgtata tgttcctgtt cgattatgga
     2881 attctataca catgccccta aatgatatgc agattcgccg tgctaaacct gaagctaaag
     2941 cctatacact tggggatggg caagggttgt ctttacttgt agagccaaat ggaagtaaaa
     3001 gttggcgatt tcgttaccgc tatgcaggta agcctaaaat gatctcgctt ggtgtctatc
     3061 caacgatcac acttgctgaa gctcgttctc gccgtgatga ggcacgaaaa atagtggctg
     3121 agggaaaaaa tcctagtgag gttcgaaaag aacaaaaact agcattaaga attcaatctg
     3181 agaacgcatt cgaaaagatt gccagagagt ggcatcagat gaaatcggca aaatggtctg
     3241 ctgggtatgc ctcagatatt attgaagcgt tccagaatga tatttttccg tatgtggggg
     3301 cgaggcctgt aggggaaatt aaaccgctag aattgctaaa tgtactgcgt aaaattgaga
     3361 agcgtggtgc attggagaaa atgcgcaaag tgcggcaacg ttgctcagag gtttttcgat
     3421 atgccattgc tactggaaga gcagagttta atcctgcggc agatctgtcc agtgcccttg
     3481 aagttcataa atcaaatcat tttcctttct taaaatcaga tgagatacct gattttctgc
     3541 gtgctttaga tagttataca ggaagccgac ttgttcagat tgccacaaaa ttattaatgg
     3601 ttacgggggt tagaactatc gagttacgag cagcattatg gcaagagttt gatctagata
     3661 acgctatttg ggaaattcct gctgagagaa tgaaaatgcg taggccgcac cttgtcccct
     3721 tatcgacgca ggcgctaggc tttcttaatg aactcaaatc tatgacagga aattatcgtt
     3781 atgtttttcc tggacggaat gatccaaata aacctatgag tgaggcaagt gttaatcaac
     3841 ttatcaaacg aataggctat gctggaaaac ttacaggaca tgggtttagg catacattat
     3901 caacaattct tcatgaaaat ggctttaata ccgcttggat tgaaatgcaa cttgctcatg
     3961 tagataaaaa ctctataaga ggaacatata atcacgccca atattttgaa aagcgaaagg
     4021 t
//
LOCUS       DAZFAH010000289         3975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFAH010000289 DAZFAH010000000
VERSION     DAZFAH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.291"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..380)
                     /locus_tag="ACHJWB_004610"
     CDS             complement(<1..380)
                     /locus_tag="ACHJWB_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4316093.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIP"
     gene            complement(407..838)
                     /locus_tag="ACHJWB_004611"
     CDS             complement(407..838)
                     /locus_tag="ACHJWB_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4316094.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(852..1604)
                     /locus_tag="ACHJWB_004612"
     CDS             complement(852..1604)
                     /locus_tag="ACHJWB_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316095.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(1612..2007)
                     /locus_tag="ACHJWB_004613"
     CDS             complement(1612..2007)
                     /locus_tag="ACHJWB_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4316096.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(2004..2537)
                     /locus_tag="ACHJWB_004614"
     CDS             complement(2004..2537)
                     /locus_tag="ACHJWB_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316097.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELASALKQQLRIAIKR"
     gene            complement(2553..2906)
                     /locus_tag="ACHJWB_004615"
     CDS             complement(2553..2906)
                     /locus_tag="ACHJWB_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4316098.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(2899..3282)
                     /gene="gpFI"
                     /locus_tag="ACHJWB_004616"
     CDS             complement(2899..3282)
                     /gene="gpFI"
                     /locus_tag="ACHJWB_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4316099.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            complement(3334..>3975)
                     /locus_tag="ACHJWB_004617"
     CDS             complement(3334..>3975)
                     /locus_tag="ACHJWB_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4316100.1"
                     /translation="QAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGKKWSEQ
                     DRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQLETA
                     VKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIRCYGAI
                     QDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          878 a         1252 c          914 g          931 t
ORIGIN      
        1 ggaatccccg ctgaagccat cattaatgcg gcatcatcca ccatgacatc cgccacatcc
       61 gcagacgata aaatatcgcg ccctccgtcc ccaccgaacc ggacgcctcc ggcaagtcct
      121 gccgctttct gcatcagcat tttgtcctca tccggcctct ccacctgctc ttcctcatgc
      181 cgggggacaa gcagactgaa atcagaggga tgcatatccg gatcgcaaaa aaacaggctg
      241 agtacagcgt acgtcagccc ggaaaaatgc atatccagct gggtatcctg aaaataatgc
      301 gtgcggtaaa aacggtgcca gtcggcatat tcggtggatg tcatcccggc aagcatggcg
      361 cgccagtcgg gtctccccat ctcacgcgcc agtctgaggg caaagttcag ctcgccgtcg
      421 aagactttcc cgcagaaaaa tcatcatcag tcagcgtgtt atttttcgcc acttcagtaa
      481 tatcagtatc cggacgaaca gcttcgatca tcccggacag gcacaacacc acgtcttccg
      541 cccgggcaat ggcatcggca ggccaggtgg tgagcacttc ctgctctatc ttcatcacgg
      601 cctcattcat tgacggtgac tgcgttttct gtggatggtt atgccacagg gacatcgcca
      661 ccagaaacgc gccggttctg acgagatctt ccacgcttac ctgcaggttg ccgctggatt
      721 ctgcctgttc tgcacgccgt ttcaggaggg caagatgctc gatacgctgc agcgcagaca
      781 attcggaaag cgtgacagac acaccgttat attcaaattg ttctgttttc agaaacatgt
      841 atgacctccg tttaccctgc agcgcccgct tcagtaacgg tgacttcagc cactgcggcg
      901 aactgaccat ttccgctcac cacagggatc tgcaccttac ctgtcgccac gccgtttacc
      961 gtaattgtca tatctttcac actaatggtg gctttcgacg gatcggcgga aaccgctctg
     1021 aacgtcttgt ctgttgcact ttccggctca aaagaaaccg tcagggtggt tgttttccct
     1081 tttgccaccg taccggatgt cggcgtcacc ttaatcgcac tgaccggcgt aattttgctg
     1141 cgttcttccg ctacagaagg tttacccacg ttagtgactt tcaccgtgcg ggtgatcact
     1201 tctttcgccg tcacggcctt accgatactg ctgacccagc cacgaaacac atccaccgtg
     1261 ccattcggaa aacggatttt ataggcccgg acatcgccgc tttcaaacca gcctataagc
     1321 cctttctggc cttcctctcc cggtttccag gccagcgtaa aactggtatc acctgcagat
     1381 ttctgcccct gcccggtcgc ggtccagtcc gcgtcttcat catccaggta gttatcatcg
     1441 taggattctg ccgtcatctc gcccggcgtc agatccttca ccttagccag tcgctgccag
     1501 tcatcgtctg acaacgggtt tgcataagca tcacccttgc cgttgtaaac ccacagagtg
     1561 gtaccggcac cttttaccgg ctcaagggga tttggtgttg ccatatcgtc ctcacatctc
     1621 gtatgtaatg gaataagtca gatctgcaga actccataac gccatatcgt catcacgacg
     1681 atactcatag ccctgcgtaa ccatcgtggt aatcagtcct gccagtgccg ggatcgcggt
     1741 catcgccggg taaatccggc tttccatcca ctgatcaagc tctgaatccg gtacctgtgc
     1801 cggtaaaaac acctcaatat gcagcgtggc ccgccaggta tctgcatcca gctcttcacc
     1861 ggtatactct gcatccgtca gataaaccgc gatcgcagga aaatcctctt cgtcaaaaac
     1921 aacggggcga ccatcaaaca gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac
     1981 tgcggcacga atgtcagtgt gtttcatcgt tttattgcaa tcctcagttg ttgtttcagc
     2041 gcggatgcca gttctttagg caggcgttca cgccggatac ggtcaacatt ctcatcaaat
     2101 gcctgtttca gtggggccgc catcgggatt ttcaccacat caatggggta acggtttttc
     2161 ccggccacac gctgcatgac atgccagcga ccatttttta atcgctgaat aaatgcccgc
     2221 tgataccgat gctgaccggc tttaagtatg ctgttcgggc gacgccccgg catcctgatc
     2281 cccagcttaa tcacagggag atcaccgcgg ttaacgataa ttctggcatt cggatttctg
     2341 accgtcgccc gtttcagtct ggaccgttcc ttaaccagtt tccggcgaac ctttgtctcc
     2401 cgggcaacct gtgatgaaga ctgattaatc gccgttgtgg ccacgcggtt aatggccatt
     2461 gctgaagccg ccggaatggc gtttttacga acccggctca gattgtcaat cgcctgatca
     2521 agccctttta tcgccataat ttcaccctgc gtttatcgtc gccggttaac tgcgggtggt
     2581 tgaccacggt tgagccagag ataacagctg cccccgtcat ccggagaaac acgatccacc
     2641 cagaatatct caccattaat ggtcagcgtg tcaccacgcc gcacggcacg aaccgtatcc
     2701 gtccgcacaa ataatgacgg gctgcttcct tcaatacgga ccccgccacc ggcaaacccc
     2761 agcgactccg gatcgtcaaa aaccccctga acttcgctgc cacactgtgc ccccgaggtg
     2821 aactgcgcac agagccccat cacttcaaca atcgtgctgt ccaccccggc gagggcagca
     2881 tcaaaggcat tctgaaaatc acgcatgctc agccgttccg tgctgtatca tggctgtcgc
     2941 cagtggtgat ggcaccagaa cacgcatgcc ccggtacgtc agttcaacgg gacggcctgt
     3001 ctccgggcaa taccccatca catgcaggca tttccgcaca cggaccgctt taacatcatc
     3061 cgtagcatca gtgttgtgca actgctcacc atcgtctgtg tgattttgct caggcccgct
     3121 ctcatcacca ggcataatgc cctcccggga agcagcaagc tcctcttccc actcagacac
     3181 acgttgcgca atatccgcag cactccccga catatccgcc tcgcgcccca gcaggccagc
     3241 cagttgacga agacgtttct gattttcttc ttttgttgcc atatcagccc cctgtgaaaa
     3301 aagacacggg ggcatttcgc ccccgctcac ggattatttc acctgcacca ccacaaactc
     3361 atccgggtcc ggcagcacca tcagcggtgc ggactgcgtc atggtaaatt cacgggccgg
     3421 atcgcccacg gtcagccagt gtttcgggta acgggaagaa gccaccacac cttcggacaa
     3481 cgcctgcgca tcctgaatgg caccgtaaca acggatccca tctgcagccg tatttcccag
     3541 gaccagcgtg ccctccggca gataacgttt ttcggtaccg tcctctgcca cataagacgt
     3601 tttcgccacc acaatggcca gatcgccgta ataccctttg aaagacacca ccgcccccag
     3661 gtctttcact gccgtttcga gttgtgaatt tgagccgcga cgggtatcca gtttttcgcg
     3721 gaacagctta aaaccattca gcagacgcca gacagtaccg tccataatgg caatattcac
     3781 aagaccgctg gcctgatcgc agtagaggtc aatatcatgc gtcggatcaa acgtatcacg
     3841 gtcctgctca gaccattttt taccgtcggc ctgctcaatg ttatttcctt cagagcgtcc
     3901 aaaatccacc tcgacagtat caaactgatc cccttccatg gtgtatttgc catacagcac
     3961 ggcattcacc gcctg
//
LOCUS       DAZFAH010000290         3962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFAH010000290 DAZFAH010000000
VERSION     DAZFAH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.292"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            411..974
                     /locus_tag="ACHJWB_004618"
     CDS             411..974
                     /locus_tag="ACHJWB_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4316101.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            1129..3489
                     /locus_tag="ACHJWB_004619"
     CDS             1129..3489
                     /locus_tag="ACHJWB_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO4316102.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3651..>3962)
                     /locus_tag="ACHJWB_004620"
     CDS             complement(3651..>3962)
                     /locus_tag="ACHJWB_004620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316103.1"
                     /translation="DYHYKKAAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDTAYDD
                     RMTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMANYQLDEQLLEGCR
                     P"
BASE COUNT         1022 a          809 c         1011 g         1120 t
ORIGIN      
        1 taccatcatt tttatggtga agttaaatat ctgtagaggc ctcaagctgg gtcgctgttt
       61 ttacttaatg gtacagaagt attaatagcg gtgttgcctg tggctacccg atactggctt
      121 ttgtgaatgt cagattactt cctgtaagtg attgaatggt acttccttaa actcaattct
      181 atgaggtgtg tttttaccat tgtaattcgt actggtcctc agtttaagtg agtcttactg
      241 tactctgaaa tattacctct ttggagtaat gaggtaatac gaaacagttt tatcaggttg
      301 tctgtgctgc tggttacctc gtttattaat gccgcggtac ggcgtttctg tactctcagt
      361 atttatgtaa ttccaccttc ctgtaacttt tataaagtaa ggactataaa atgtataatg
      421 ctaatcccaa ttacgaaatg gacttcatga ttctgaaaga cgtgaatgaa cacatggaag
      481 gtctgtttca acgtttttct aaactgttac ctttcagaat tgattttgcc tacaggaaag
      541 acacgcccag ttttggtcat tcctgtaggc acagtatgtg tattgaaatg caccgcttac
      601 ttagtgaaac ccaaacgatg ctggcgggat attactgggt aatggaatat acgtcaaaca
      661 agggacttca tattcatttt atcggctacc tggatgggca acgtcacaac aaatcttatc
      721 ggatatccag gcaactggga gatatctgga ggcggatcac ggaaggggag ggttattttc
      781 acctgtgcag ggctaaagac aaatacccgg tgcgtatcga tcacgtaatt cactatagtg
      841 ataaatctgc ggtagacgac ttacgttacg cccttagcta tctggccaaa caggatcaga
      901 aagaacacgg tattattctg aggcgtagca ggcttcccga aaaaagtaac cggggccgac
      961 cacgacataa ctgaattaca gctgtattag gtattagaat taagtttctg taagttgttg
     1021 ggaggtgctt gagttaatag gcagaagagg gcggggcgct ttgtcatcag tgtaggggac
     1081 ccgaatttct gccgtggtgc ctcagtggat cggatacagg taacgaatat ggttcataaa
     1141 tctgacagtg atgaattagc tgcattgagg gcagaaaatg cccgtctggt ctcattactt
     1201 gaagctcatg ggattgaatg gcgacgtaaa ccgcagactc ctgtgcagcg cgtttctgta
     1261 ttatccaccg acgagaaggt tgcattattt cgtcggttgt ttcgtgggcg tgatgatgta
     1321 tgggcactta gatgggaaag taaaaccagc ggcaaatcag ggtactctcc tgcctgcgct
     1381 aacgaatggc aggcgagaat atgcggtaaa ccccggataa aatgtgggga ctgcgctcac
     1441 cgtcagttga ttcctgtatc cgatctcgtc atctaccacc atctggccgg tacccatact
     1501 gtcgggatgt atccgttgct ggaagatgat tcctgttatt ttctggcagt tgattttgat
     1561 gaagctgaat ggcagaagga tgcatccgca ttcatgcgat cctgtgatga gctgggtgta
     1621 cctgctgcgc tggaaatatc ccgttcacgt cagggggcgc atgtctggat attttttgcc
     1681 tcacgagttt cggcccgcga agctcgccgt ctggggactg ctattattag ctatacgtgt
     1741 agtcggaccc gacagctgcg attggggtct tatgaccgat tatttcctaa tcaggatact
     1801 atgccaaaag ggggatttgg taatctcata gcgttacctc tgcaaaaaag accgcgtgaa
     1861 ttggggggaa gcgtttttgt tgatatgaat ctccagcctt atcctgatca gtgggctttt
     1921 cttgtatcgg tgaccccgat gaatgtgcag gatattgaac cgacgatatt acgggctaca
     1981 gggagtatcc atcctctgga tgtgaatttt atcaacgaag aagacctggg tacgccgtgg
     2041 gaagagaaaa aatcatcagg aaacagactg aatatttcta ttgcagaacc gctgaaaatc
     2101 acgctggcaa accagatcta tttcgaaaaa gcgcaattac ctcaggtgct gattaaccga
     2161 cttattcggc tggcagcatt tccgaaccct gagttttata aggctcaggc aatgcgtatg
     2221 tcagtctgga ataagccccg tgttataggt tgtgcggaga attacccgca acacattgcg
     2281 ttgccccggg gatgtctgga cagcgtatta tctttcctta gggacaacaa tattgctgca
     2341 gaattaatcg ataaacgatt tgccgggacg gaatgtaatg ccgtttttat gggaaacctc
     2401 agagcggagc aggaagaggc cgtttcggca ttactccgtt atgacactgg tgtgctttgt
     2461 gcgccaacgg cttttggtaa gacagttacc gcagcggcag tgattgccaa gcggaaagtg
     2521 aatacactga tactggtaca ccggactgaa ttgctgaagc agtggcagga gcgtctcgcg
     2581 gtgtttctgc aggccggtga cagtattggt attatcgggg gaggtaaaca taaaccctgt
     2641 ggcaatattg atattgcggt ggtgcagtcc atatccagac aaggagaagt tgaacctctg
     2701 gtcaggaatt atgggcaaat cattgtggat gagtgccatc atattggcgc ggtttcattt
     2761 tctgcgattc tgaaggaaac gaatgccaga tatctgcttg gcctgacggc aacaccaatc
     2821 cgacgggatg gtctgcatcc cattattttt atgtactgtg gtgccattcg ccatacagcg
     2881 gtccgcccga aggaaagccc acataatctg gaggtactga tccgttcccg ttttacatct
     2941 ggtcatttac catcggatgc gagaatccag gatattttca gagaaattgc tctggatcat
     3001 gacagaacgg tggcgatagc tgaagaagcc atgaaagctt tcgggcaggg gcgaaaagtt
     3061 ctggtactga ctgaacgtac agatcatctg gatgagatag catcagtgat gaattcactg
     3121 aaattgtctc cctttattct ccatggtcga ctatcgaaaa aaaagcgtgc gatgctgata
     3181 tccgggctga atgctcttcc tcccgattct cctcgaattt tgttgtcaac aggcagactt
     3241 attggtgagg gatttgacca ccctccgctg gatacgctga ttcttgccat gcctgtgtca
     3301 tggaaaggga cattacagca gtatgcaggg cgtcttcaca gagagcatac cggtaaaagc
     3361 gatgtcagga tcattgattt tgtggatacc gcgtatcctg tgctgctcag aatgtgggat
     3421 aaacgtcagc ggggttataa agcgatgggg tacaggatta tagctgacgg cgatgaatca
     3481 gtgatttaat tttaaggagt actttaccct ttttaaataa tttgagctcc ccgggttgtt
     3541 cgggcaacaa agagtcccag tatacaggct tcaggaatat gttgtagtat caggcttttc
     3601 atcgctttaa aatgactacc ggcagtgaga acgtcatcaa aaataacaat ctagggcctg
     3661 cacccttcca gtaattgttc atccagttgg tagtttgcca taatcgcttc tggtaagggc
     3721 cggtttgttg cggtatgaga ggctattgtt gatacgtttt gcacaaccag ttcccagaaa
     3781 tccagccatg gttcgtaggt ttgcgcaatt cgcagaattt ctgtcatacg gtcatcgtaa
     3841 gcggtatctg tacggcattt ggatggtggt acgggaacaa atgtgtattc ctcaagatta
     3901 ccgatatttg atatgagcat ctgagcaaca tactcaatag cagccttttt atagtggtaa
     3961 tc
//
LOCUS       DAZFAH010000291         3802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFAH010000291 DAZFAH010000000
VERSION     DAZFAH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.293"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..436
                     /locus_tag="ACHJWB_004621"
     CDS             <1..436
                     /locus_tag="ACHJWB_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4316104.1"
                     /translation="EQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            477..899
                     /locus_tag="ACHJWB_004622"
     CDS             477..899
                     /locus_tag="ACHJWB_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4316105.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNTRKKLSRAKLKKPAVVDPDLIWSLPDG
                     EIRRYDRHQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            896..1192
                     /locus_tag="ACHJWB_004623"
     CDS             896..1192
                     /locus_tag="ACHJWB_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4316106.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            1189..1650
                     /locus_tag="ACHJWB_004624"
     CDS             1189..1650
                     /locus_tag="ACHJWB_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO4316107.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            1628..1984
                     /locus_tag="ACHJWB_004625"
     CDS             1628..1984
                     /locus_tag="ACHJWB_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316108.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRVTKSRTE"
     gene            2035..2247
                     /locus_tag="ACHJWB_004626"
     CDS             2035..2247
                     /locus_tag="ACHJWB_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316109.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            2280..2497
                     /locus_tag="ACHJWB_004627"
                     /pseudo
     CDS             2280..2497
                     /locus_tag="ACHJWB_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            2499..2762
                     /locus_tag="ACHJWB_004628"
     CDS             2499..2762
                     /locus_tag="ACHJWB_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316110.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            2773..2898
                     /locus_tag="ACHJWB_004629"
                     /pseudo
     CDS             2773..2898
                     /locus_tag="ACHJWB_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3454..3642
                     /locus_tag="ACHJWB_004630"
     CDS             3454..3642
                     /locus_tag="ACHJWB_004630"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4316111.1"
                     /translation="MPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WIPSHWMPLPEPPQEVRQ"
BASE COUNT         1090 a          768 c         1063 g          881 t
ORIGIN      
        1 ggagcaatca caccaacttc agcagcctga agccgattcc gccattcagc gggaagccga
       61 tcgggtagtc ccggaaaaca ccggtcagtc tgtgggacga gtggattatc cggatgtgtt
      121 cgaacaggtc tggcgggagt acccgttgcg tgccggagca aacccgaaga aatccgcatt
      181 cagtgcctgg aaggccagat tacgcgaggg ggtgccacca gaggccatgc tggatggcgt
      241 gaggcgttac gcaagatact tggcggctac cgggaaaacg ggaacggaat ttgttcagcg
      301 agcgacgacg ttttttggac cggaccggaa ttttgagaac ccctggttgc tcccggtaag
      361 cggcacgaac aaccagcgtt gtgtgaatca tatttctgaa ccggataacg aaattccgcc
      421 gggcttcagg gggtaagtgt taatttctgg tcatgaggta attttcagga gggcttgtgg
      481 caaaagtttt tacacaagaa gagcgggaaa aaattaaagg gcaggttctt gaactcgtac
      541 gccagagtgg gcgcgagacg ttacgacaac tggaagctaa aactggggca acaagatatc
      601 tgatgagcgt tctggccaga gagctggttg ccagtggcga tgtatacaac tctggttacg
      661 ggttattccc gtctgaacag gcgcgtaagg actggcaaaa tacccgtaaa aagctctcaa
      721 gggcaaagct gaagaaacct gcagtggttg atccggacct tatctggtcg ttaccagacg
      781 gcgaaatacg ccgctacgac aggcaccaga acataatttg tcgtgagagc aggaggagcg
      841 aagttatgca gcgtgtgctg gcgttctatc ggggaaactt tcaggaggtg atggagtgag
      901 ggtgagagtt tatattgccg gtccaatgac gggatatgaa aatttcaacc gtgaggcatt
      961 tcacaaggtg gaagaggaac tgaaacggga agggcatacc gtcttaaacc cggcagtact
     1021 tccggacggg ctgacacagc cgcactacat ggatatttgc atggcaatga ttcgttgtgt
     1081 ggatgcgatt tacatgctga atggctggca gcggtcagcg ggcgctaagg cagagctggc
     1141 actggcggag aaactggggc atgcggtgat ttatcaggag gtggctcaat gagagaggtt
     1201 aactatgagg cgcttcgtga ggcagcacaa aactatcagt cgacgctggc gtggtatcag
     1261 gctaccccgg acagcccaaa tgctgaacgg gattgtgatg cggctcttgc tgcgtttaag
     1321 cgtcatatcc gtcatcggga agcggatatt atcgctggtt tgctggatgg actggaagaa
     1381 gcaaaatcac aactcaacga gcagcgtgag tattacgaag gcgttatctc tgatgggagc
     1441 aagcgtattg ctgaactgga tgcgcgggaa gttcaattac cgactcgcta cgaccttcga
     1501 tatggacacc cgataaatgc agatgagcga catgtcatga tacctaaaga aaatggcagt
     1561 tggctttacc tgattgacct agaacacgca ttacgcgtcg ctggcattcg catcaaagga
     1621 gaggagcatg gaaataaaac cagaggatga gttaagcaat atcgttttat ttccggtaaa
     1681 agaggatgac cctcgtaatc aggttaattt tctttatgag ccatcggaaa gaccatattg
     1741 tcatcacgcc tctgtccggg ttgacgaaaa agagcgtcag gtccgctgta aaatctgcgg
     1801 tgcagttgtg gagccatttg actggatgct ctctgtggcg aaaagagaaa ctaggctggc
     1861 agatgatgta aagctattgc gccaggagga acaggaaagg cggaaaaata tagaaaagtt
     1921 aattcagatt gagcgtaacg cgaaagcgcg gatacgcagg gtgacaaaat ccagaactga
     1981 ataaataaat ttagcgctgt aaataaaatc taatccttaa ctggaggtgt atttatgtta
     2041 aatacacaga aagccattaa tgcggaaaaa tataacgagt gggcaagaaa attctctgag
     2101 cagattttta aaattactgg cgatgagaat gcggcaaaaa atgaattaga accgtggacg
     2161 cctgaaggag ccgacccaaa ttattgctgg agggaggttg atccagttga tgctgcaaat
     2221 gaagctatga gttatcacaa cgattaatgt caggaggccg cccgaaaggg cggtaatgaa
     2281 tggtcacatt atttagaaaa aatatccgcg aaagagtaga acaacagaat ttctgtttct
     2341 cattctgttt atcgtgttga tgataccgat atccccgtta atcctagtct ggataatcgg
     2401 aaaaataatt gagccagtta ttgaattgta taacgacgtg gtatgggcgt cattcaacac
     2461 actgcacaat aaaattaatc cgtataagga aaactgatat ggcaactttg acaaaaaaag
     2521 aacgggcatg gttgaacgag ttacaggaag ttcttgatcg ctgtccatca ccgaaaaaaa
     2581 ttggctttta caccattggc gataaaagca tttacctgta tgacctacgc cgcatggatg
     2641 aaatcatgga ggctcttgat aatcgttcgt cgatggattg gtgtgttgct gttcatgata
     2701 tgaatgcagg gtttgatgaa aagattttgt tcccctcatc agttgaaagc actgcgggtt
     2761 aaggagtaac acatgaccac tattaccaaa gaacgtattg aattgttcat taaaaatccg
     2821 cttgaaaacg ggcttacccg tggtgaacaa atggaactgg cacggattgc gctggcatcg
     2881 ctggaagcag agccggtggc aaagattata gctcattacc cattaggggt tgacgtaggc
     2941 aaacaaaaat ttgtacaggc cattagagag cttcctgact ttggcggata tctatttgcc
     3001 gcccctccag cgccgatagt gccggaagaa atgtattggc aggatgcgcc agttgaaggc
     3061 agcagcaaag cggctgcata cgctacaggc tggaacgatt gccgcgaagc catgtttcag
     3121 tccggaaact ttcgggaaaa taaagattcg tcaaccaata attttcggaa aatcccggaa
     3181 gcgtcaacca gctctccggt aactccggct cttctgcctg gtggtttcac cattgaggag
     3241 gcgaaggaat tacatgaaga cctggcacgc agccacataa gcaaggcctt aagtggcgaa
     3301 aagatgaaaa agaaagatcg cgatgctgat ttgcgctgga ttcatggcgt tatagttcag
     3361 gcagcgtggt ttgtaaaagc atcactggag cagaatgcac tatcgggcaa ctatccggta
     3421 actccggata gttggataag ctgtagtgag cgaatgccgg ataccaaaac agccgttctt
     3481 gttgccaggg attttggcag gaaaggtgac tggcgaatga aatgggcgac ttacatcccg
     3541 gggcatcctg acgctaatga tgggtggata atacctggtg cgtcgtggat accatcacac
     3601 tggatgcctc taccagaacc gccgcaggag gtgcgccaat gaactggcct gaagcatttg
     3661 caattacagg cgttgctatg gctatcgctt ttttagtata tgttatttgt cggtgagggt
     3721 aaaaacgttc gccgggatta acaccaaagg agggaatatg tcggatgata tatcactggc
     3781 aatggaaggt gcgctggctg tt
//
LOCUS       DAZFAH010000292         3782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFAH010000292 DAZFAH010000000
VERSION     DAZFAH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.294"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..293
                     /locus_tag="ACHJWB_004631"
     CDS             66..293
                     /locus_tag="ACHJWB_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316112.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(370..612)
                     /gene="yfdY"
                     /locus_tag="ACHJWB_004632"
     CDS             complement(370..612)
                     /gene="yfdY"
                     /locus_tag="ACHJWB_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4316113.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            965..1885
                     /gene="lpxP"
                     /locus_tag="ACHJWB_004633"
     CDS             965..1885
                     /gene="lpxP"
                     /locus_tag="ACHJWB_004633"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4316114.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2241..2312
                     /gene="ypdK"
                     /locus_tag="ACHJWB_004634"
     CDS             2241..2312
                     /gene="ypdK"
                     /locus_tag="ACHJWB_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4316115.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2377..3615)
                     /gene="alaC"
                     /locus_tag="ACHJWB_004635"
     CDS             complement(2377..3615)
                     /gene="alaC"
                     /locus_tag="ACHJWB_004635"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4316116.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          976 a          954 c          886 g          966 t
ORIGIN      
        1 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
       61 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
      121 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
      181 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa
      241 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
      301 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
      361 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
      421 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
      481 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
      541 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
      601 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
      661 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcatgt
      721 taaatgcaag actgttgtgt acggaaaaat atttactttg cacgattaat aatcagtagc
      781 tgaaagcagt cagcgagtga taaagtacag ataccgcatt catgctcctc gtggttatgt
      841 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
      901 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
      961 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
     1021 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
     1081 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
     1141 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
     1201 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
     1261 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
     1321 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
     1381 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
     1441 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
     1501 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
     1561 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
     1621 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
     1681 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
     1741 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
     1801 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
     1861 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
     1921 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc
     1981 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
     2041 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
     2101 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
     2161 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
     2221 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
     2281 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
     2341 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
     2401 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
     2461 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
     2521 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
     2581 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
     2641 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg
     2701 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
     2761 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
     2821 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
     2881 gcttttcgac agcgtaaaga actcgactgc cacatcgcgt gcacccggta cctgcataat
     2941 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
     3001 cacatcgtag cgtttcgcca gcgccactac tttttcgaag aactccagtt ccacgcattg
     3061 cgcggttggg tttgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
     3121 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
     3181 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
     3241 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
     3301 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
     3361 gcgggaaatg gcgcgacgta agcgcggaat gccgcgtgac gtggagtaac cgtgcgtgtc
     3421 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
     3481 gttacccata ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
     3541 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
     3601 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
     3661 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
     3721 gaacattttt atcaatggct ttaaatgagg aactccactt caaaaacagg aaatttttct
     3781 ac
//
LOCUS       DAZFAH010000293         3753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFAH010000293 DAZFAH010000000
VERSION     DAZFAH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.295"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /locus_tag="ACHJWB_004636"
     CDS             <1..276
                     /locus_tag="ACHJWB_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4316117.1"
                     /translation="EHDLHTLSTQQKADCVVLNPEKSHPYIEDMAMFLSPLPTVLEWH
                     CVMCEGLFHGKKFCEACGTGIYCRATCTKYVYRYQPDLYQDCGGALT"
     gene            464..897
                     /locus_tag="ACHJWB_004637"
                     /pseudo
     CDS             464..897
                     /locus_tag="ACHJWB_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            979..1146
                     /locus_tag="ACHJWB_004638"
                     /pseudo
     CDS             979..1146
                     /locus_tag="ACHJWB_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            1440..1916
                     /locus_tag="ACHJWB_004639"
     CDS             1440..1916
                     /locus_tag="ACHJWB_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316118.1"
                     /translation="MRRFVQQAVDHWPNLLAFHFTFHSTEGNIYGQQIHAFCISFYQQ
                     IHKRITARTHTTSPSSPLVLHWLLEQHGGAVIRCVLLLSQTSICHPRASVTVDEECSK
                     LVDLLQQIWPVISTGGQCQVERCFQVARGDTSEQYVALKTDALSFAVSDHKSSGAE"
     gene            2089..3316
                     /locus_tag="ACHJWB_004640"
                     /pseudo
     CDS             2089..3316
                     /locus_tag="ACHJWB_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          938 a          880 c         1002 g          933 t
ORIGIN      
        1 gagcatgatt tacataccct gagtacgcaa cagaaggcgg actgtgtggt gctgaatccg
       61 gaaaagtccc acccttacat tgaggatatg gcgatgtttt tatcgccatt accgaccgtg
      121 ctggagtggc attgtgtgat gtgcgagggg ctttttcatg ggaagaagtt ctgtgaggcc
      181 tgtggtacgg ggatttattg cagagcaaca tgtacgaagt atgtttacag gtaccagcca
      241 gatttgtacc aggactgcgg aggtgcactc acttaatccg aaatgcccgc tctgctcagg
      301 gcgttcaggc tgactatcaa tggcatacat tttatccggt gtttcgcacc cacaccgtgc
      361 aacgactcga caccggatat ccgcagtccc gcttacctga ataaaacccg ggggattgat
      421 tgtgtactgt ggtgtgaaat cgcaccgtga tcgctgattc gccgaatccg cactgtggtg
      481 tcaatcaacc acacgcgaga gtcgtctcag gcttcgcctg cctcaggacg atttctgcat
      541 agctaattcc cggggcaaca tttatagagg cgctgtgcgc tctgatggtc gccacttctg
      601 acccggacca tgctcctgat atgtcggaat ataatgtgat gttaggcatc agagaggaat
      661 atttcgatgc ccctaatctg gggcgtttga ttctggatgt ctggagcaat ccgggctttg
      721 tgtcaccgga cgaatcaaag cagtaccacc cgggatgtgg cgctggatcg tcattcaccg
      781 gactgtgaag acatacttaa agagctgata tgggcgttca gtgatttcgc ccgcgatcat
      841 cgtggtgtgt gtgatccgga agcccgttac ctcgccggta atccttggta tccgtagcag
      901 gccagctctg aaagcacagc tcagcagatt ccccgcattc gcacaacctt cataaatctc
      961 acagaggaca ttgtggccat gttgaccaca acaagccacg acagcgtatt gctgcgttcc
     1021 gatgatcccc tgctcgacat gaactacatc accagtttca ccggcatgac cgacaaatag
     1081 ttttataaac tgatcagtga aggctatttt caaactgggc ggtacaaaag cgaagtggag
     1141 cagtggatgc agcagcgaat agagggagcg tgggggcagc aggatgaaac gtgttgtgat
     1201 gccggtacgc tggtattgtg caaaatgccg gcgctactat tgtggaaatc agccctgtcc
     1261 ctggtgctgg cgatactccc tgcagacatt ctccggtcag ccaataggtt gttgctcttt
     1321 cctgatcgat tcgtcattcc attctttttg tttataatgg gcattacact ggtgttggcg
     1381 tgtttacctg cgtgtctgca ccggttcagt aaaattagaa aagttcagag tcggggaaaa
     1441 tccggcgttt tgttcagcag gcggtggatc actggccaaa tctgttggca tttcacttca
     1501 cgttccattc gacagaagga aacatctacg ggcaacagat tcatgcattc tgcatttcct
     1561 tttaccaaca gatacacaaa cgtattactg cacgtactca cacgaccagt ccatcatcgc
     1621 cgttggtgtt acactggctg ctggaacaac atggaggagc agtaatacga tgcgtattgc
     1681 tcctcagcca gacgagtatt tgtcatccgc gagccagcgt cacggttgat gaagaatgtt
     1741 cgaaactggt ggatttattg caacagatct ggccagtgat aagcactggc ggacaatgcc
     1801 aagtggaaag gtgttttcag gttgcccggg gtgatacatc agagcaatac gttgcgttaa
     1861 aaacagatgc gttgtcgttt gctgtgtcgg atcacaaatc atccggtgca gagtagcatt
     1921 ctggctgtat tctggtaaat cagcgtatta tcgatatatt atcgtactat cagaaaccgt
     1981 atcgtctgtt gatggctaga ctggcccccc tgaatcttca gacaaccaat atcacttatt
     2041 taagtgatag tcttactact agtttttaga ctagtcattg gagaacagat gattgatgtc
     2101 ttaggaccgg agaaacgcag acggtgtacc actcagaaaa aatcgatatt gttcagcaaa
     2161 gttttgaacc gggaatgaca gtctcccttg ttgcccggca acacggtgtg gcagccagcc
     2221 agttatttct ctggcgcaag caataccagg agggaagtct tactgctgtg gccgcaggag
     2281 agcaggtcgt tcctgcctct gaacttgctg ctgccatgaa gcagattaaa gaactccagc
     2341 gcctgctcgg aaaaaaaacg atggaaaatg aactccttaa agaagccgtt gaatatgggc
     2401 gtgcaaaaaa gtggatagcg cacgcgccct tattgcccgg ggatgaggag taaacgtcgt
     2461 cagtcgttgt cgccgggtgt cgcatgagca gttgcacatc attctcagac gaagtgatga
     2521 ctggaaggat ggccgccgca gtcttcacac tgatgatacg gatgtgcttc tccggataca
     2581 tcatatcatc ggagagttgc ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag
     2641 acaggcagaa cttgatggta tgtctgcgat caatgccaaa caagtttacc ggatcatgcg
     2701 ccagaatgcg ctgttgcttg agcgaaaacc cgctgtaccg ccatcgaaac aggcacatac
     2761 cggcagagtg gccgtgaaag aaagtaatca gcgatggtgc tctgacgggg tcgagttccg
     2821 ctgtgataac ggagagagac tgcgtgtcac attcgcgctg gactgctgtg accgtgaggc
     2881 actgcactgg gcagtcacta cgggcggctt caacagtgaa acagtacagg acgtcatgct
     2941 gggagcggtg gaacgccgct tcggcaacga gcttccggcg tctccagtag agtggctgac
     3001 ggataatggt tcatgctacc ggcctaatga aacacggcag tttgcccgga tgttggggct
     3061 tgaaccgaag aacacggcgg tgcggagtcc ggagagtaac ggcatagcag agagcttcgt
     3121 gaaaacgata aagcgtgact acatcagtgt catgcccaaa ccagacgggt taacggcagc
     3181 aaagaacctt gcagaggcat tcgagcatta taacgaaagg catccgcata gtgcactggg
     3241 ttatcgctcg ccacgggaat atctacggca gcaagccagt aatgggttaa gtgataacag
     3301 gtgtctggaa atataggggc aaatccagcg acaaagaaca gggttaatga atctgtaacc
     3361 agtcccacct tagcgctatg cacttttgaa tcacagagct tgctaatcgt ttgtccgact
     3421 ttaacagagt taatgacggc gatgatctga tgccaagtaa aacggacttg atgtatggca
     3481 gatcctcttt cgccgaatgg atatgggcaa tacttcttaa tacgaatggt acggttatgc
     3541 gggaagagac taacgcggtt acttgttcag ctatttgtcc cttgttcctt tttataatgc
     3601 acccgttcca ggttagtgct ggctgtagct tatgtccggg agacagctaa tagatatata
     3661 tactcgtcat acttcaagtt gcatgtgctg cgactgcgtt cgcttacccc aatcacttac
     3721 ttatgtaagc tcctggggat tcactcgctt gcc
//
LOCUS       DAZFAH010000294         3750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFAH010000294 DAZFAH010000000
VERSION     DAZFAH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.296"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1388
                     /gene="dcuC"
                     /locus_tag="ACHJWB_004641"
     CDS             3..1388
                     /gene="dcuC"
                     /locus_tag="ACHJWB_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4316119.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMPHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1429..2109)
                     /gene="dpiA"
                     /locus_tag="ACHJWB_004642"
                     /gene_synonym="citB"
     CDS             complement(1429..2109)
                     /gene="dpiA"
                     /locus_tag="ACHJWB_004642"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4316120.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2078..3736)
                     /gene="dpiB"
                     /locus_tag="ACHJWB_004643"
     CDS             complement(2078..3736)
                     /gene="dpiB"
                     /locus_tag="ACHJWB_004643"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4316121.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVVNFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESKAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYITKVKPNDSSINPIDR"
BASE COUNT          859 a          986 c          908 g          997 t
ORIGIN      
        1 ttatgctgac attcattgaa ctccttattg gggttgtggt tattgtgggt gtagctcgct
       61 acatcattaa agggtattcc gccactggtg tgttatttgt cggtggcctg ttattgctga
      121 ttatcagtgc cattatgggg cacaaagtgt taccgtccag ccaggcttca acaggctaca
      181 gcgccacgga tatcgttgaa tacgttaaaa tattactaat gagccgcggc ggcgacctcg
      241 gcatgatgat tatgatgctg tgtggatttg ccgcttacat gccccatatc ggcgcgaatg
      301 atatggtggt caagctggcg tcaaaaccat tgcagtatat taactcccct tacctgctga
      361 tgattgccgc ctattttgtc gcctgtctga tgtctctggc cgtctcttcc gcaaccggtc
      421 tgggtgtttt gctgatggca accctatttc cggtgatggt aaacgttggt atcagtcgtg
      481 gcgcagctgc tgccatttgt gcctccccgg cggcgattat tctcgcaccg acttcagggg
      541 atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat tgacttcgcc ttcaaaacga
      601 cactgcctat ctcaattgct gcaattatcg gcatggcgat cgcccacttc ttctggcaac
      661 gttatctgga taaaaaagag cacatatctc atgaaatgtt agatgtcagt gaaataacca
      721 ccactgcccc tgcgttttat gccattttgc cgttcacgcc gatcatcggg gtgctgattt
      781 ttgacggcaa atggggtccg caattacata tcatcactat tctggtgatt tgtatgctga
      841 ttgcctccat tctggagttc ctccgcagct ttaataccca gaaagttttc tctggtctgg
      901 aagtggctta tcgcgggatg gcagatgcat ttgcaaacgt ggtgatgctg ctggttgccg
      961 ctggggtatt cgctcagggg cttagcacca tcggctttat tcaaagtctg atttctatcg
     1021 ctacctcgtt tggttcggcg agtatcatcc tgatgctggt attggtgatt ctgacaatgc
     1081 tggcggcagt cacgaccggt tcaggcaatg cgccgtttta tgcgtttgtt gagatgatcc
     1141 cgaaactggc gcactcttca ggcattaacc cggcgtattt gactatcccg atgctgcagg
     1201 cgtcaaacct gggccgtacc ctttcgcccg tttctggcgt agtcgttgcg gttgccggga
     1261 tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc ggtgccggtg cttgttgggc
     1321 tggtgattgt tatcgttgct acagagctga tggtgccagg aacggcagca gcggtcacag
     1381 gcaagtaaat agtaatgccg gaggcaagtt tcctccggct ttatcatgtc acccactgtg
     1441 gtatatgcgt tgcggtctgc caactttgcc gtgaacaatt tcagcaataa tcagatggcg
     1501 gctggcgcaa tattcaagat aacgcctggc agtggtgcgg ctgatggtca gtgcctgcgc
     1561 caccgtttct gccgtatgtt gcacaccagg ctctttgaac agttttcgca ccgcgtttag
     1621 cgtcaaaggg tcaatgccgg tcggcagttc gtctttgggt tcaccgcgag cataggcatt
     1681 aaacatctca tcaatttgct tctggctggc gctatcaata ctttccagca tatgtttacg
     1741 ctggcggaaa cgggttagcg tttgccccag ccgttcatag gcaatgggct taatgagata
     1801 atcaaataca ccacaacgta cggcttcaga caccgtttcc atatcgctgg ctgcagtggt
     1861 aaacaccacg tcgccgggat aatgcgcctg caccagttca tgcaataaat taatccctct
     1921 accgtcagga agatagttat cgagcaagat tagccccggc ttaaaacgct cgatcatcat
     1981 tcgggcctgc gccaggtttc ccgccagtaa tatctgactg aatccgggaa tgtgacgaat
     2041 atattccgca tgcatctctg ccagcggcgt ttcgtcctca acgatcaata gggttaatgg
     2101 agctgtcatt aggtttcact ttcgtaatat agattgaaaa taaggtaccg cagggatcat
     2161 tatcttcgag agtgataaca ccaccgcagc gcgttacgta gctggcaatc aagtacaacc
     2221 caatgccatg ttcaccgggc tcgtcagcac gcgtactgac cccctgctca aatattttgt
     2281 ctcgtagaga ctctggaacg ccgcagccct gatcggcgac ttcaatcacc acatcatcgc
     2341 cttcatcgct gaggaataat tcaacgatct tgtttccttc atcgctacgc aggctggctt
     2401 cgaaggcgtt atcaagtaaa ttgccaacaa tggcggcaaa ctcggtgcta tccagtcctg
     2461 gcggcagttg cgaaagctgg ctaccgggga cgatgatcat ttttagcccc agttcgcggg
     2521 cgcgctgcac tttaccaaaa agcagccccg ccacctggcg atcggcaaac gcctcacgca
     2581 ggctgtcaat aagctgttgc tgggccttag actccccctg caccatcgcc agcacgcgat
     2641 catactcttt catctgcaac agaccattga gcgtcgacat ccaattgaga tgctcgtgtc
     2701 gcaatgtacg aaggctctca acgtattgtt ttatttgcgt cagttgcgca ttgagggtgg
     2761 aaatttcgtc tttactacga aagctgataa tggcccccag caaatcatca cctgaacgaa
     2821 tagcttcccg gttggcaata acgctcagac cgttaaagtt caccaccaca tcctgacgtt
     2881 tttcatcaat ctgttcggta aagaaatcgg cgggcctgac cacttcagca atgggtttac
     2941 ccaaccattg ccgtccaggg gaactcagcc ccagcatctt tcttgcgtta cgattgatgg
     3001 cggtaatgta accgtgcgga tccaccgcaa tcagcccttc ataaaccgaa ctaaacagcg
     3061 cctcttgctg acggaccacc cgtgcgattt gctttggctc catgcccatc atctgccgac
     3121 ggatatgcgc ggccaggaac cacgacagca acatcagaat ccctaacagc acgacaaaca
     3181 cgcctgccat cggtaataaa aactcagccc gccagctatc gattttactc accaggtagc
     3241 caatcgacac cacgccgatg acttttccat cgtcatcaaa gattggcgtt ttggcgcgca
     3301 tcgccatacc cattgaccct ttcccggtaa tgaagtagct ctcccctttc tccagcgcgc
     3361 cctgtttggt gaactgcata ggataaccaa ttttctccgg attaggatgg taaaggcgga
     3421 tcgagtgccg gtcgccaatc accacataat caaaatcggt atctctttgt aatttgttag
     3481 cgatggtcgc cagccgtttg tagtcacgcg ttttcaccgc agagatgaca ctgtcattgg
     3541 aggcaataat tttcgcctga ttcattgcca tgtcgcgtac atgaagcgtt aaatagtcct
     3601 caaaactggc cgtaaaatat tgcgccaggg ctgcaatgac aaatattgag aacaccagaa
     3661 tcagcaaaaa gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct
     3721 cgttaagctg caacattgtt ttccttgcgg
//
LOCUS       DAZFAH010000295         3659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFAH010000295 DAZFAH010000000
VERSION     DAZFAH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.297"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..358)
                     /locus_tag="ACHJWB_004644"
     CDS             complement(80..358)
                     /locus_tag="ACHJWB_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4316122.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            complement(360..551)
                     /locus_tag="ACHJWB_004645"
     CDS             complement(360..551)
                     /locus_tag="ACHJWB_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO4316123.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRTKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            complement(572..943)
                     /locus_tag="ACHJWB_004646"
     CDS             complement(572..943)
                     /locus_tag="ACHJWB_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316124.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            1041..1343
                     /locus_tag="ACHJWB_004647"
     CDS             1041..1343
                     /locus_tag="ACHJWB_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4316125.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            1340..1765
                     /locus_tag="ACHJWB_004648"
     CDS             1340..1765
                     /locus_tag="ACHJWB_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4316126.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            1788..2750
                     /locus_tag="ACHJWB_004649"
     CDS             1788..2750
                     /locus_tag="ACHJWB_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316127.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            2757..3497
                     /locus_tag="ACHJWB_004650"
     CDS             2757..3497
                     /locus_tag="ACHJWB_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4316128.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            3523..>3659
                     /locus_tag="ACHJWB_004651"
     CDS             3523..>3659
                     /locus_tag="ACHJWB_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316129.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KT"
BASE COUNT          939 a          811 c         1000 g          909 t
ORIGIN      
        1 aactaaaccg cgggacataa caaacacaac gcttttgata aagtcgttgc ggttgttatg
       61 tttctattgg tagtgaaagt tagggaaact ggcgtcttgc gtggatcacg tttactactt
      121 caacgcttga tgttgttacg cggtatagaa ttatatagtt agggtgggct acaatctcac
      181 gcaagccagg tactctgtcg cttggtgggt ataaatacgg atgttcggat aacggcagca
      241 cacaacccct taatcgctgc cataagcgtt cagccgcatc tatgtcgaaa cgagcaatat
      301 aactagttat atcatctagg tcggtatctg cgctttcaag ccataacacg ggtaacattt
      361 tactgcttgc tccgttcctt gcgcatctta gcaaagcgtt ctgccattct gcgctcaact
      421 tcgtcatggg gaattgctgg gcgcggatct gcaaggctcg ttgctacttt cgtacgcagc
      481 cattcgttgt aactgttttc ttgttcaatg gtttcaaatt cagaaaccat tggtgaaagg
      541 gctctattca tgtttcctcc ggttttataa ctcaggcgcg gcggcatttt tgcgccgcaa
      601 tccatctcgc tatgagatct tccattgatt cttttttctg ctttaactcg ctgattatct
      661 ggcgttgctc atcctcaggg aaggctgaaa aaatctgcaa taattccagt tgattagacg
      721 ttaaccctgc atgtggtgga gaaacccccg gttgttctgc gtattccgca tccagatacc
      781 cttccggcat cccgtatgtt tgctctattc ttctggcagc cttttctcca aacgaggctc
      841 tcccactcat tagttgagat aggtagctct tctctttggg tggcagagtt ttatctttaa
      901 accactcctt gagacgtaaa cggcgaattt cttttttttg catgtggtaa ttatctttag
      961 taatcactaa acaagcaaat acttgactta atggtttatt aaacactaaa ctcgcaaaaa
     1021 aacactaaac cgaggaaggt atgacattaa aagagtttat taaatcatta agggttggtg
     1081 atgctaagaa attcgcggcc agacttggtg tatcgccatc ttacttatcg caaatggcgt
     1141 ctggacgaac agctatatct ccaacccgcg cccttatgat cgaatctgcg acggaaggcc
     1201 aagtaagtag ggcggagcta cgaccccatg attgggagct tatttggcct gagtatgcga
     1261 gcggcattcg tttggggcaa acacatgtag ttcatgctga aggtgattgt agtgcatgct
     1321 tatctgatgg agttgattca tgaaaatcaa gcatgaacac atccgcatgg cgatgaatgt
     1381 ctgggcgcat ccggacggcg aaaaagtacc ggctgcgaaa attaccaaag cgtatttcga
     1441 gctgggaatg acgttcccgg aactgtatga cgacagccat ccggaagccc tggcccgtaa
     1501 tacccagaaa attttccgtt ggctggataa agacacccct gatgctgttg aaaaaatgca
     1561 ggctctgtta ccggcgatcg aaaaggcgat gccgcctttg ctggtggccc gtatgcgcag
     1621 ccacagttct gaatattacc gtgagatcgt cgaacggagg gatcggctgg tgaaggatgt
     1681 cgatgatttt gttgcgtcag cggttgtttt gtatgaccag atgaatcgcg gcggcccggc
     1741 agggaatgct gtggtgatgc actaaaagca cggtgttcgg gggttttatg agcagcaagc
     1801 ttcatggtct tgtctgggaa gggtgcgcct tcaccggcat gatcttatcc agggtggcgg
     1861 ttatggcccg tcttgcagac tacagcaatg acgagggcgt gtcatggcct gccattgaaa
     1921 ctatccggcg tcagatcggt gcaagaagtg aatccacagt gaaatcggct attgcagaac
     1981 tggcgaaaga gggctggctg acgaaggaag agcgtaaggt cggtgggcgt aatgtaagca
     2041 atatctatcg gcttaatgtg gaaaaactcg aagcagctgc tgcggcggcg cgtgagtcat
     2101 ataaaccgaa aagaaaaatt agcccggcaa aaaatgaccc gttaacagtt gacccgtcaa
     2161 atattgcccc ctcaacggtt gacccgtcaa attttgatgg atcaactgtt gataacaaac
     2221 tgccgattag gggggcgatg attgaccccg atccgtcagt attaaaacct gatccgtcag
     2281 ataaaagatc ttcttgtccg gacgcttcgc aaccggaccc gcagacggct gaacagaatt
     2341 ttttaacccg acaccctgac gcggttgtgt tcagtgcgaa aaaacgccag tggggaagtc
     2401 aggaagattt ggtgtgcgca cagtggatct ggggacgaat cgtgagtctt tacgagcagg
     2461 cggccagcga tgatggcgag atcactagac cgaaagaacc caactggaca gcatgggcca
     2521 atgacgttcg cacaatgcgg atgctggatg gcagaactca cagacaaatt tgtgaaatgt
     2581 ttgggcgtct ccagcgggat tcgttctggg taaaaaacat catgagtccg gcaaaactcc
     2641 gggaaaaatg ggatgaactg gttatccgcc tggggcgttc gcctgcgcag cgttgcgtga
     2701 atcacatttc tgaaccggac actgaaatac cgccgggatt cagggggtga cgtgtcatga
     2761 aaaacattgc ggcagttggg gttcttgaac gtattcgcag acttgcacca cagggggcgg
     2821 ttccaccgta ccggacggtg gaggagtggc gggaatggca acttgctgaa ggacgaaaac
     2881 gcagcgagga gattaaccgc ctgaatcatc aggtgcgggt tgaaaaaatc ctgaaccgtg
     2941 cgggcatcca gccgcttcac aggaagtgct cattcgggaa ctaccgggtg cagaacgacg
     3001 gtcagcgcca tgctctgagc caggcgaaat ccattgccga tgaattgatg accggatgta
     3061 caaacttcgt gttcagcggt aaacctggta ccggtaaaaa tcacctggca gcagcgattg
     3121 gcaatcggct gatggcgaag gggagaagcg tgattatcgt caccgtgtcc gatgtcatga
     3181 gcgtgttgca tgacggctac gacaacggcc agtccgggga aaaattttta caggagcttt
     3241 gtggagttga ccttctggtc cttgatgaaa ttggcatgca gcgggatacg cgcaacgagc
     3301 aggtcacgct gaaccagata gtcgaccgca gaacggcttc gatgcgtagt gtcggaatgc
     3361 tgacgaacct gaatcacgca gcgatgagca cactcctcgg agatcgggtg atggaccgta
     3421 tgaccatgaa tggtggtcgt tgggtgaatt ttaactggga gagctggcgg tcaaacgttg
     3481 gacgtcaggg tatgtgagaa tttttgacga ggtaaatttt cgatggaaac tgtattgcat
     3541 gcactgaaag cgatgggaaa agccaattct gttgaactgg cggcgcggct tgatatcagc
     3601 cgtgaagaag ttcttaacga actgtgggaa ctcaaaaaaa atggcgttgt tgataaaac
//
LOCUS       DAZFAH010000296         3619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFAH010000296 DAZFAH010000000
VERSION     DAZFAH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.298"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..533
                     /gene="lpfB-O113"
                     /locus_tag="ACHJWB_004652"
     CDS             <1..533
                     /gene="lpfB-O113"
                     /locus_tag="ACHJWB_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO4316130.1"
                     /translation="YLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKG
                     VSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKV
                     KWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWR
                     FINDYGAVSDPIKMTL"
     gene            558..3080
                     /gene="lpfC-O113"
                     /locus_tag="ACHJWB_004653"
     CDS             558..3080
                     /gene="lpfC-O113"
                     /locus_tag="ACHJWB_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO4316131.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISAWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3091..>3619
                     /gene="lpfD-O113"
                     /locus_tag="ACHJWB_004654"
     CDS             3091..>3619
                     /gene="lpfD-O113"
                     /locus_tag="ACHJWB_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO4316132.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLII"
BASE COUNT          997 a          776 c          870 g          976 t
ORIGIN      
        1 cttatctcat ccaggcttgg gtagatccat ttaataaaga ggataaaagc aaagcgccgt
       61 ttaccgttat tccgccagta tcacgattag aaccttcgca agaaaaaata ttacgcatta
      121 ttcacaccaa aggtgtgtca ttgcctgatg atcgcgagtc agtcttctgg ctgaatatta
      181 aaaatattcc gccatcagcg agcaacaaag cgacgaactc gctggaaata gcagtaaaaa
      241 cgcgtatcaa gctgttctgg cgaccagcaa atatccgcct tattcccgaa gatgctgcgc
      301 cgaaagtgaa atggcgccgg gaagggcgaa atctgatagc agagaatccc aacccaattc
      361 atatcagtgt gatggatgtc atcgttgatg gccatgatgt gcccctcaat atgatccgtc
      421 cttttgaaac gctcacgtta cctttacctg ctaattctgc gggaggccaa atgacctggc
      481 gatttattaa tgattacggc gcagtcagtg acccgataaa aatgacgctg taagcaaggg
      541 ataaggatag gcgtattatg atgacgacca gaatagtggt tggcctcacg gcagggacgt
      601 gtctgatttt ctcgcaaaac ctgatggccg aggtcagtgt attcaatccg gcgcttctgg
      661 aaatcgacca tcaatccgga gtcgatattc gccagtttaa tcgggcaaac ctgatgcccc
      721 caggtgttta tagcgttgat atttttatca acggtaaaat gtttgaacgt caggatgtga
      781 catttgttca ggataatcca gatgctgatc tgcacgcttg ctttattgcc attaaaaaaa
      841 cactgtcctc ctttggcata aaagttgatg cgctcaaatc gttcaatgat gtggatgaga
      901 cggtttgcct cgatcctgct ccacgtattg aaggctcatc ctggcagttt gacagtgata
      961 aattgcagct gaatatatcc attccccaaa tctacatgga cgcgatggct tatgattaca
     1021 tcagccccac gcgttgggat gaggggatta acgcgctcac catcaactac gatttttctg
     1081 gttcacatac actacgttca gattatggtt cacaagagac agataccagt tatctcaatc
     1141 tgcgcaatgg actgaatatt ggaccgtggc ggctacgtaa ttacagtact ttaaacacca
     1201 gcgatggccg tgcggaatac aactccatta gtgcctggat acagcgcgat attgccgcgt
     1261 taagaagcca gattatgatt ggtgatacgt ggacggcgag cgatattttc gacagtacgc
     1321 aaattcgcgg cgcgcgtttg tatactgata acgatatgct acccgccagc cagaatggct
     1381 ttgctcctgt ggttcgtggg attgcaaagt ccaacgccac cgtcatcatt cggcagaatg
     1441 gctacgtgat ttatcagtct gccgttccac aaggtgcttt tgagattacc gatctcaaca
     1501 ccgccagtac aggtggcgat ttggacgtaa ccatcaaaga agaagacggt agcgaacaac
     1561 gattcaccca accttatgct tcattggcga ttcttaaacg tgaaggtcag acagatgttg
     1621 atgtcagcgt gggtgaattg cgcgatgaag acggatttac accggacgtc cttcaggcgc
     1681 aaatacttca tggtttttcc cacgggatca ctttatatgg aggtatgcag gctgctgaaa
     1741 attatggttc tgcagctctg ggtgtcggta aagatcttgg cgctttgggc gcaatttctt
     1801 tcgatgtgac acatgctcgt gcgaatttta gccatgatga tacagaaacg ggtcagtcat
     1861 atcgctttct ctattcaaaa cgatttgacg acacagacac tagcttgcgc ctggttggct
     1921 atcgttactc caccgagggc tactataccc tcaatgagtg ggcatcgcgg cgcaacagcc
     1981 ctgaagactt ttgggaaaca ggtaaccgac gtagtcgcgt ggagggaacg ctaacgcagt
     2041 cgttggggag agattatggc aatttatacc tgacattaag ccggcaacaa tactggcata
     2101 ccgatgatgt cgaacgatta atgcaatttg gctacagcag tagctggaag cgtctctcgt
     2161 ggaacgtctc ctggagttat tccaatactg ccagacaggg gacggggaac aaccatgcca
     2221 gtgataacac cagtgagcag atctacatgc tctctttatc tgttccttta tcgggctggt
     2281 ggggtaatag ttacgccacc tattctgttt cgcaaaacga taattccggt agctcacatc
     2341 aactcggact cagcggtacg gcgctggaaa gaaataacct ttcatggaat ttaatgcagt
     2401 cctataacag tcatgatgat gaggttggcg gtaatatgtc cctgacctat gatggctctt
     2461 atggcacggt gaacggcagc tataactaca gccaaaattc ccagaggctg aattatggta
     2521 tcagaggggg aattctggca cacagcgaag gggtaacgtt aagtcaggag ttaggtgaaa
     2581 ctattgctct tgttaaagca cctggggccg ccgggttaga aatagataat atgcgcggtg
     2641 ctgcgacgga ttggcgcggc tatacggtca agacacagct aaacccttat gatgaaaatc
     2701 gggtagcaat cagcgataac tatttctcga agtcgaatat agaacttgat aataccgtcg
     2761 ttacgatggt tcccacgcgt ggtgcagtgg ttaaagcgga gtttgtgact catgtgggtt
     2821 atcgcgttct cttcagagtg ttaaatgcaa atggtaaacc ggtacctttt ggagccattg
     2881 ctgcgataca agatgcaagt ttggcagatt caggaattgt cggtgaccgt ggcgaacttt
     2941 atctttctgg tctaccagaa aaaggacagg ttacgttatc ctggggagaa aacgcctcaa
     3001 caaaatgcat cttcaattat tcactttcga caccagaaag tgagagcgga ttaattgaac
     3061 agggtgtgac atgtcattaa aaggattttt atgaacaagt atatcaaaca gtggtgcttt
     3121 gctgtgttta tgctctcgtt aagtagtgta gcacttgcag ctcctaaagg tatctgtacc
     3181 cctgataatg gcgtatttca cagtacgctt gatttctcgg gatatcttat taccgcgaac
     3241 gaaaataagg ttggaacgac atttaacacc actgtaacta atggatcctc atatcctgga
     3301 cgctgtcatt gtgatactgg taacgtagga gaattcccct atatctatta tacgtcaaaa
     3361 ataaaccagg cattaactta tgctggggtt cactctaata ttaattatta tgatcttaat
     3421 ccgaaccttg atgttgggat tgctatagat attcttgggg ttgggtatgt gaatgcacct
     3481 tttgaatatc atgctaacaa cccgtctggt aatacaaagt acaattgcaa ccgcattgaa
     3541 cctttaagta tatctagtgg ggctaaagcg atagtatatt tttatatcaa gaagacattt
     3601 gcaggaaaat tgattattc
//
LOCUS       DAZFAH010000297         3617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFAH010000297 DAZFAH010000000
VERSION     DAZFAH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.299"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            257..2191
                     /gene="yeaG"
                     /locus_tag="ACHJWB_004655"
     CDS             257..2191
                     /gene="yeaG"
                     /locus_tag="ACHJWB_004655"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4316133.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2304..3587
                     /locus_tag="ACHJWB_004656"
     CDS             2304..3587
                     /locus_tag="ACHJWB_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4316134.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT          974 a          881 c          906 g          856 t
ORIGIN      
        1 aattatgcaa aatttatgga tgagttgttg atatgccatt gaaattaaga aagccgtgca
       61 ggcaagtttt ccatttgcca tctacgctta attttgaagg tgtatcaccg ggcacgttgt
      121 tctcatcgtc gataaaatgg catgagagtt gctgtgtttt agcaagagac gtcgttcagt
      181 ttacctcttc cgggagcctc tactattcat atgaacggct cttaacctgt gctaaaaaac
      241 gaaaggacgg cataccatga atatattcga tcactatcgc cagcgatatg aagctgccaa
      301 ggacgaagag ttcacactgc aggagtttct taccacttgt cggcaagatc gcagtgctta
      361 tgccaacgcg gctgagcggc tattgatggc tatcggtgag cctgtcatgg tcgatacagc
      421 ccaggaaccc agactttctc gactcttttc taaccgggtc attgcacgtt atccggcatt
      481 tgaagagttt tacggcatgg aagacgcgat tgaacagatt gtctcttatc tgaaacacgc
      541 ggctcagggg ctggaagaga agaaacaaat cctgtatctg ctggggcctg tgggtggggg
      601 taaatcatcg cttgctgagc gactgaaatc attaatgcag ctcgtaccga tttatgtatt
      661 gagcgccaac ggtgagcgta gcccggtcaa cgatcatccg ttctgtcttt tcaatccgca
      721 ggaagatgct cagattctgg aaaaagagta tggcattcct cgccgttatc tcggcaccat
      781 catgtcgcca tgggcggcaa aacgcctgca tgaatttggt ggcgatatca ctaagttccg
      841 ggtagtgaag gtctggccgt caattctgca acaaattgct atcgccaaaa cggaacccgg
      901 tgatgagaac aaccaggaca tctccgcgct ggttgggaaa gtcgatattc gtaaactcga
      961 acactacgcg cagaatgacc cggacgccta cggctattcc ggtgcgctgt gccgcgccaa
     1021 tcaggggatc atggaattcg ttgagatgtt taaagcaccg ataaaagtgc tgcatccctt
     1081 gttaaccgcc actcaggaag gtaactacaa cgggacggaa ggtatctccg ccctgccgtt
     1141 caacgggatt attctcgccc actcgaacga gtccgaatgg gtcactttcc gtaataacaa
     1201 aaacaacgaa gccttcctcg accgtgttta catcgtgaag gtgccgtatt gcttgcgcat
     1261 ttccgaagag atcaaaatct acgagaaatt gcttaatcac agtgaattga ctcacgcccc
     1321 ctgcgcccct ggcacgctcg aaacactgtc acgtttttcc attctttcgc gcctgaaaga
     1381 gccagaaaac tccagcattt attcaaagat gcgggtttat gatggcgaaa gtctgaaaga
     1441 caccgatccc aaagccaagt cgtatcagga atatcgtgac tacgccggtg tcgatgaagg
     1501 gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc tcccgcgtgt tcaacttcga
     1561 tcatgtagaa gtggcggcaa acccagtcca tctgttctac gtcctggaac agcagatcga
     1621 gcgcgagcag ttcccacaag agcaggcaga acgctatctg gagttcctga aaggttatct
     1681 gatcccgaaa tatgccgagt ttatcggcaa agagatccag acggcctacc ttgaatccta
     1741 ttccgaatat gggcaaaaca ttttcgaccg ttatgttacc tacgcggatt tctggattca
     1801 ggatcaggag tatcgcgatc cggataccgg gcagctgttt gaccgcgagt ctcttaacgc
     1861 cgagctggag aaaatcgaga aaccggcggg gatcagtaat ccaaaagatt tccgcaacga
     1921 gattgttaac ttcgtactgc gcgccagagc gaataacagc ggacgcaatc cgaactggac
     1981 cagctatgaa aaactgcgca cggtcatcga gaagaaaatg ttctccaata ccgaggagct
     2041 gttgccggtt atctcgttta acgccaaaac gtcaaccgac gagcagaaga aacacgacga
     2101 ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa caggtgcgtt tactgtgcga
     2161 atggtatttg cgcgtacgta aatcgtctta acaaccctgg cccggtacac atgttaccgg
     2221 gcctacaacg acaacgaacc gtgggcctga gaagcggcaa cacaggcgta gcatacagtt
     2281 ggcaaatgta gtacgggggg catatgacct ggtttattga ccggcgtctg aacggcaaaa
     2341 acaaaagcat ggtgaatcgc cagcgttttt tacgccgtta taaagcgcaa attaaacagt
     2401 cgatctccga ggccattaat aagcgttcgg tgactgacgt cgacagcggc gaatccgtat
     2461 ccattcccac ggaagatatt agcgaaccga tgtttcatca ggggcgtggc ggtctgcgcc
     2521 accgcgtgca tccgggcaat gaccatttcg tccagaacga ccgaattgaa cgtccccagg
     2581 gtggcggcgg aggttccggc agtggtcagg gccaggccag ccaggatggt gaaggtcagg
     2641 atgaatttgt ctttcagatt tcgaaagatg agtatcttga tttgctcttt gaagatttgg
     2701 ccttaccgaa tctgaaacaa aaccaacaac gccagctgac cgaatataaa acgcatcggg
     2761 cgggttatac tgctaacggc gttccggcca atatcagcgt tgtgcgttcc ttgcagaact
     2821 cactggcgcg acgcacagcc atgacggcag gcaagcggcg ggaacttcat gcactggaag
     2881 agaatttggc catcatcagc aacagtgaac ctgcgcagct gctggaagag gaacgtctgc
     2941 gcaaagaaat tgcagaatta cgtgccaaaa ttgaacgcgt cccttttatt gacaccttcg
     3001 atttacgtta caagaactac gagaagcggc ccgatccctc cagccaggca gtgatgtttt
     3061 gcctgatgga cgtttccggt tcaatggatc aatccactaa agatatggct aagcgttttt
     3121 atattctgct gtatctgttc ctcagcagaa cgtataagaa cgtggaagtc gtatacatcc
     3181 gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt tttctactcg caggaaacag
     3241 gcggcaccat tgtttccagc gccctgaaac tgatggatga ggtagtgaaa gagcgttata
     3301 acccggcaca gtggaatatt tacgctgcac aagcatcgga cggcgataac tgggccgatg
     3361 actctccgct ttgccatgaa atcctggcga aaaaattatt acctgttgtt cgttattaca
     3421 gctatatcga aattacccgt cgtgcacatc agacattgtg gcgagaatat gagcatctgc
     3481 aatctacttt cgacaacttt gcgatgcagc acatccgcga ccaggatgat atttatccgg
     3541 tgttccgtga actgtttcat aaacaaaatg caacagctaa agactaaaac tatcagccag
     3601 gtcattatcg cctggct
//
LOCUS       DAZFAH010000298         3600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFAH010000298 DAZFAH010000000
VERSION     DAZFAH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.300"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1082
                     /locus_tag="ACHJWB_004657"
     CDS             <1..1082
                     /locus_tag="ACHJWB_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4316135.1"
                     /translation="GLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEE
                     YQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETH
                     RQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKG
                     IAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDAS
                     GKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEGGIKELTVNGEWQADEYGNQWRQ
                     ASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGART
                     SMSENELDILTRYFQYNAKDITEKQ"
     gene            1107..3536
                     /gene="torZ"
                     /locus_tag="ACHJWB_004658"
     CDS             1107..3536
                     /gene="torZ"
                     /locus_tag="ACHJWB_004658"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4316136.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQNIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
BASE COUNT          983 a          820 c          993 g          804 t
ORIGIN      
        1 ttgggttatt gtttttgctg atagcggttg tggttggtgg cggcgggtta ttgctggcgc
       61 aaaaagcctt acataaaacg tcggatacag cattttgcct ttcctgccac tcgatgagta
      121 aaccttttga ggaatatcag ggaactgtcc acttttcgaa ccagaaaggg atacgtgcgg
      181 aatgtgccga ttgccatatt ccaaagtcag ggatggatta tttatttgct aaattaaaag
      241 catctaaaga tatttatcat gaatttgtta gcggcaaaat agacagtgac gataagttcg
      301 aaactcatcg ccaggaaatg gccgaaacag tatggaaaga attaaaagcc actgactctg
      361 caacgtgccg tagttgccat tcttttgatg ccatggatat tgcctcgcaa agtgaatctg
      421 cgcagaaaat gcataacaaa gcacaaaagg gcggcgaaac ctgtatcgat tgtcataaag
      481 gcattgccca ttttccgcca gaaataaaaa tggatgacaa cgcggcgcat gagctggaaa
      541 gtcaggccgc tacttcagtg actaatggcg cacatattta tcctttcaaa acttctcgca
      601 taggcgagct ggctaccgtg aatcctggta ccgatctcac cgtcgttgat gccagtggca
      661 aacagccgat cgttctgttg cagggttatc aaatgcaggg cagtgaaaac acgctctacc
      721 tagcggcagg tcaacggctg gcgctagcca cattaagtga aggaggtatc aaggagctca
      781 cggtaaacgg ggaatggcag gctgacgaat acggcaatca atggcgtcag gcgtctttac
      841 agggtgcgct taccgatccc gcattagcgg accgtaaacc gctatggcaa tacgctgaaa
      901 aacttgacga tacctattgc gctggttgtc atgcccctat tgccgccgac cattacaccg
      961 tcaatgcgtg gccgtccatt gccaaaggaa tgggggcacg aaccagcatg agcgaaaacg
     1021 aactggacat tttaacgcgg tatttccagt acaacgccaa agatattacc gagaaacagt
     1081 gatcctcgtt gatcagggag gaagttatga cattaacaag acgtgaattt attaaacaca
     1141 gcggtattgc cgctggagcg ctggtggtga catcggcagc accgctcccc gcatgggcag
     1201 aagagaaggg cggtaaaatc ctcaccgcgg gacgttgggg agcgatgaac gtagaagtga
     1261 aggacggcaa gattgtttct tcaacaggcg cgctggcgaa aaccataccg aattccttac
     1321 agtctacggc ggcggatcag gtacacacca cggcgcgtat tcagcatccg atggtgagaa
     1381 aaagctatct cgataatcca ctgcaaccgg cgaaaggtcg aggcgaagat acctatgtac
     1441 aggtgagctg ggaacaggca ttaaaactga ttcatgagca acatgagcgt attcgtaaag
     1501 ctaacggacc atcggccatt tttgccggat cttacggctg gcgctcttct ggcgtgttgc
     1561 ataaagcgca aaccttactg caacgttata tgaacctggc gggcggttat tccgggcata
     1621 gcggcgatta ttcaaccggg gcagcacagg tgatcatgcc gcatgtggtc ggttctgtag
     1681 aggtgtatga acagcagacc tcctggccgc tgattctgga aaacagccag gttgtggtgc
     1741 tgtggggaat gaacccgctg aataccttaa aaattgcctg gagcagtacc gatgagcaag
     1801 ggctggaata cttccatcag ctgaaaaaat ctggcaaacc agtgattgcc attgatccta
     1861 tccgctccga aaccatcgaa ttctttggcg ataatgccac ctggatcgcg ccgaatatgg
     1921 gcaccgacgt ggcactgatg ttagggattg cgcataccct gatgacacaa ggcaaacatg
     1981 ataaagtgtt ccttgaaaaa tacaccaccg ggtatccgca gtttgaagag tatctgacag
     2041 gtaagagcga taatacgccg aagagcgcgg cctgggcggc ggaaattact ggcgtaccgg
     2101 aagcgcagat agtcaaactc gctgaactga tggcggcgaa tcgcacaatg ctgatggcag
     2161 gctggggaat tcagcgccag caatatggtg aacagaaaca ctggatgctg gtgacgctgg
     2221 cggcgatgtt agggcagatt ggtacaccgg gcggcggttt tggtttctct tatcattact
     2281 ccaatggtgg caacccgacg cgtgttggcg gcgtgctgcc ggaaatgtct gcggcgattg
     2341 ccggacaggc cagcgaggcc gctgatgatg gcggaatgac ggcgattcct gttgcccgta
     2401 tcgtcgatgc gctggaaaat cctggtggca aatatcagca taacggtaaa gaacaaacgt
     2461 atccgaatat caaaatgatc tggtgggcgg gtggcggtaa ctttacccat caccaggata
     2521 ctaatcgtct gattaaagca tggcagaaac cggagatgat cgtcgtttct gaatgctact
     2581 ggaccgcagc tgctaaacat gcagatatcg tattaccgat caccacatcg tttgagcgca
     2641 atgacttgac gatgaccggt gattacagca accagaatat tgtgccgatg aagcaggctg
     2701 ttgctccgca atttgaagcg cgaaacgatt ttgacgtgtt tgccgaactt gcggaattac
     2761 tgaaacctgg cggaaaagag atctataccg aaggtaaaga tgaaatggcg tggctgaaat
     2821 ttttctatga tgccgctcag aaaggtgccc gtgcgcaacg cgtgactatg ccgatgttta
     2881 atgccttctg gcagcaaaat aaactgatcg aaatgcgccg cagcgagaag aacgaacagt
     2941 acgttcgtta tgctgatttc cgcgccgatc cggtgaaaaa tgcgctgggt acgccaagcg
     3001 gcaaaattga gatttactcc aaaacgctgg aaaaatttgg ctataaggat tgcccggcac
     3061 acccaacctg gctggctcct gatgaatgga aaggtaccgc cgacgagaag cagttacagc
     3121 ttctgaccgc acatccggca caccgtttac acagtcagct taactatgcg gaactgcgta
     3181 aaaaatatgc gattgccgat cgtgaaccaa tcactattca caccgaagat gctgctcgct
     3241 ttggtattgc gaatggtgat ctggtgcgtg tctggaacaa acgtggtcag attctgacag
     3301 gtgcggtggt gactgacggg atcaaaaaag gcgtggtatg cgtgcatgaa ggtgcatggc
     3361 cagatctgga aaatggcttg tgtaaaaacg gcagtgcgaa cgtgttaacg gcggatatcc
     3421 ccagctcgca gctggcaaat gcctgtgccg gtaactctgc gttggtgtat atcgaaaaat
     3481 atacgggcaa tgcgccgaag ttaacggcgt ttgatcagcc agctgttcag gcgtaagaag
     3541 tgaatagcgc ccggaaacgg gcgctgaggt tatgcaaacg taaagctgcc tgatgcgcta
//
LOCUS       DAZFAH010000299         3561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFAH010000299 DAZFAH010000000
VERSION     DAZFAH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.301"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..96)
                     /gene="escG"
                     /locus_tag="ACHJWB_004659"
     CDS             complement(<1..96)
                     /gene="escG"
                     /locus_tag="ACHJWB_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO4316137.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAEAILN"
     gene            complement(102..323)
                     /gene="escF"
                     /locus_tag="ACHJWB_004660"
     CDS             complement(102..323)
                     /gene="escF"
                     /locus_tag="ACHJWB_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO4316138.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            complement(358..765)
                     /gene="cesD2"
                     /locus_tag="ACHJWB_004661"
     CDS             complement(358..765)
                     /gene="cesD2"
                     /locus_tag="ACHJWB_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO4316139.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFIMNVNS"
     gene            complement(772..1734)
                     /gene="espB"
                     /locus_tag="ACHJWB_004662"
     CDS             complement(772..1734)
                     /gene="espB"
                     /locus_tag="ACHJWB_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO4316140.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTGSASSTTTSTSSISSSLLTDGRV
                     DISRLLLEIQKLLREMVTTLQDYLQKQLAQSYDIQLAVFESQNKAISEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDAVQQAASTSAKSISTASEASTKALAKASESIA
                     DAADDAASVQQTIAAAAKAASRTSGVTDDVAASAQKASQVAEEAAGAAQELAEKAGLL
                     SRFTAAAGRISGSTSFIVVTSLAEGTKTLPTTISESVKSNHDISEQRAKSVENLQASN
                     LENYKQDVRRTQDDISSRLRDMTTTARDLTDLINRLGQSARLAG"
     gene            complement(1755..2897)
                     /gene="sctE"
                     /locus_tag="ACHJWB_004663"
     CDS             complement(1755..2897)
                     /gene="sctE"
                     /locus_tag="ACHJWB_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO4316141.1"
                     /translation="MLNVNSDIQSVMSGVSAATTTSGINQPGGSSALDLQLVKSAAPS
                     ASWTESTALPKPPAGHSLVTPSVAEDVLSKLFGGISGDVMSRTSGTEPQSSTQSTTSG
                     YPYLSQVNKVDPQTMMMMATLLSLDASAQKVASMKNSNEIYKDGQNQALENKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAVAAIFNPVLWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLMSKVGDVAGKVGSNIVKAVTT
                     LADTLVENVASKISAVANGLTTSSRSIGTTVLNNDAANHNLLSQISSFVVENLTRQSE
                     SLSQSAKAELDKAALEVQNQANYIQSASKLISDSARVNSRIVSGRV"
     gene            complement(2910..3488)
                     /gene="espA"
                     /locus_tag="ACHJWB_004664"
     CDS             complement(2910..3488)
                     /gene="espA"
                     /locus_tag="ACHJWB_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO4316142.1"
                     /translation="MDTLNTASVASTNASTSTSLTYDLGSMSKDQVVELFNKLGVFQA
                     ALLMFSYMYQAQSDLSIAKFADMNEASKASTTAQKMANLVDAKIAEVQSSSDKNAKAQ
                     LPKEVLDYINDPRNNVTISGITDLNAALGAGNLQTVKAAISAQANNLTTTVNNSQLEI
                     QQMSNTLNLLTSARSDMQSLQFRTVSSISLGK"
BASE COUNT          991 a          840 c          698 g         1032 t
ORIGIN      
        1 attcaggata gcttccgcat atttgggaag cttatgattt gccgcggcta cagctgccca
       61 caccagtatc ttattggcag aaacatcatt aaccataaaa attaaaagct acggttggaa
      121 atggttgaga ccagatcttt tatcgttttc agcattccac tttctacgtt aacataagca
      181 gaatattgct gaaccgcaaa ctgcaactct aacatttttt cagggtcatt aaccaaatcg
      241 gtgctattaa gtaactctgg cacagaatcg ctcagtgttt tacctacctc acccatttgt
      301 tgagtaattt cagataaatt cattaaatct ccttaatagt taataaaata aaaaccccta
      361 actatttaca ttcattatga accaaattcc gtcttctgtt tcttgctgca cggcaccctt
      421 tctgatgaaa taattaaaca actcttcgcg cttttgacgt tctgaatctg aaccaatctt
      481 agatttcatc gcactgattt tcttaatacc cgaaaaatgg tgatacaact cttcgagcaa
      541 acgagtcagt gataaaacag ggccgttaag ccctgtttgg ttacgtgctt caaaagagca
      601 caaaatcagt tcttgttcat ccagtctgta cgctaatgag aaaaaaggtg tctcaatccg
      661 ccatccgata aaaaaggcac tgccacaaag aaactccttc tgtattgtgt accctttttg
      721 ttcaagatag tgattaagca cttcatcatt aaacgtatcg accatgatca attacccagc
      781 taagcgagcc gattgcccca ggcgattaat aaggtcggta aggtcacggg ctgtcgttgt
      841 catatcacgc agacggctag agatatcatc ctgcgttctg cgaacatcct gtttgtagtt
      901 ctctaaattc gacgcctgga gattttctac agatttagca cgctgctcac tgatatcatg
      961 attggacttg acagattcag atatcgttgt tggcaatgtt ttcgttcctt cagcaaggct
     1021 ggtaacaaca ataaatgacg tagaaccgga aatccttcca gcagcagcag taaagcgact
     1081 taataatcct gccttctctg ctaattcttg agcagcacca gcagcctctt cagctacttg
     1141 agaagctttc tgagcagaag cagccacatc atcagtgaca ccagatgtac gactggccgc
     1201 ttttgcagct gccgcgatag tttgctgcac tgaggctgca tcatctgctg catctgcaat
     1261 actttcggaa gccttcgcca gtgctttagt tgaagcttca gaggctgtac tgatagactt
     1321 cgcagaagta gaagcggctt gctgaacggc atcactcgct cctttcgttg cgctattaat
     1381 ggcagcaaaa gagcctaaaa tccccaggac agatgagata gcaccaccga tcagagcggc
     1441 tgtcgcaccg gcttttttct cactaatagc tttattttgg ctctcaaaaa cggctagttg
     1501 gatgtcatag ctttgcgcca attgcttctg aagataatct tgcaatgtgg tgaccatctc
     1561 acgcaacagt ttttgaatct ccagcagaag cctagaaata tccactctgc catcagtaag
     1621 taaagatgaa ctgattgacg aggttgatgt cgttgttgaa ctggcgctgc cagtcgtgct
     1681 gctcaaaaca gaattaactg cgattgccgc attgttatta tcgatagtat tcattataaa
     1741 cgcctcttta acaattaaac tcgaccgctg acaatacggc tattcactcg cgcagaatca
     1801 gaaattagct tagaagcact ctgtatataa ttcgcctggt tttgcacctc gagtgcggct
     1861 ttatccagct ctgcctttgc actctgactt aagctttcac tttgtcgagt taaattttcc
     1921 acaacaaaag aagaaatctg tgataacaga ttatgattcg ctgcgtcatt attcagcaca
     1981 gttgtaccaa tagagcgcga agaagtggtt aaaccatttg ccacagccga aattttcgat
     2041 gcaacgtttt cgacaagagt gtcggccaac gtagtgaccg cttttacgat gtttgaaccc
     2101 actttgccag ccacatcgcc aactttagac ataagtgaag acaccccgcc aatgccggct
     2161 gtcagaatac ctgcggcaag agatagaccg ccaaaagctt gcgctgctgt ttttaacgcc
     2221 tgcggagcct tatcgcccat aacatcaacc gcagtttgca gcgccattgc tgttgcacta
     2281 acagcaacaa cagcccagag aactgggtta aaaatagctg caacagcagt taccgcaacc
     2341 cctaaccaac caaagacctg accaacaatt ttactttttt gtgctttctc ttcggctttc
     2401 tgttgttctt caagctgttt tttaaattcc agagttttat tttccagcgc ttgattttgt
     2461 ccatccttgt agatctcgtt ggagttcttc atactcgcaa ctttctgcgc tgaggcatcc
     2521 agggataaca gagtggccat catcatcatt gtctgagggt caaccttatt cacctgagaa
     2581 agataaggat agccagaggt tgtactttgt gtactactct gtggttctgt cccactagta
     2641 cggctcataa catcaccact aatgccgcca aaaagtttgc tcaaaacatc ttcagcaaca
     2701 gagggcgtca ctaatgagtg gcctgccggc ggctttggta aagcggtact ttctgtccaa
     2761 cttgctgatg gggcagcgga tttaaccagc tgtaaatcca gcgcggaaga ccctccaggt
     2821 tgattaatac ctgaagtagt cgtagcggca ctgaccccag acatcacaga ctggatatcg
     2881 ctatttacat taagcatagt tatctccggt tatttaccaa gggatattga tgatacagtt
     2941 ctgaattgca gtgactgcat atcagaacgt gcactcgtta ataggtttaa cgtatttgac
     3001 atttgctgta tttcaagctg gctattattc accgtcgttg tcaaattatt cgcttgagct
     3061 gaaatagctg ctttcaccgt ttgcaaatta ccagcgccta atgcagcatt tagatcggta
     3121 ataccactta ttgtaacgtt attgcgagga tcatttatat agtcaagcac ttctttagga
     3181 agttgagcct tcgcattctt gtcggagcta ctctgaacct cagcaatttt agcatccaca
     3241 agattcgcca ttttttgggc agtggttgac gccttagatg cctcattcat atcagcaaac
     3301 tttgcaatcg acagatcgct ttgtgcctga tacatatagg aaaacatgag aagcgcagcc
     3361 tgaaaaacac cgagtttatt gaatagttca acaacctgat ctttcgacat gctccctaaa
     3421 tcataggtca gcgatgtcga agtactcgca ttcgtactag caactgatgc tgtatttaat
     3481 gtatccatgt atatacctct taataatctt tcttagcatg acaaaaacta ttaaaaaaca
     3541 attaaatcac ataacatcct c
//
LOCUS       DAZFAH010000300         3542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFAH010000300 DAZFAH010000000
VERSION     DAZFAH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.302"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..624
                     /gene="fimC"
                     /locus_tag="ACHJWB_004665"
     CDS             <1..624
                     /gene="fimC"
                     /locus_tag="ACHJWB_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4316143.1"
                     /translation="AGGIALGATRIIYPADAKQTAVWIRNSHTNERFLVNSWIENSSG
                     VKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDKNALNGR
                     NVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNLQVG
                     SQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
     gene            655..3264
                     /locus_tag="ACHJWB_004666"
     CDS             655..3264
                     /locus_tag="ACHJWB_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4316144.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGYW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYLVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3277..>3542
                     /locus_tag="ACHJWB_004667"
     CDS             3277..>3542
                     /locus_tag="ACHJWB_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316145.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIV"
BASE COUNT          931 a          783 c          865 g          963 t
ORIGIN      
        1 gccggaggga tcgcattagg tgccacgcgt attatttatc ccgctgatgc taaacagact
       61 gcggtatgga ttagaaatag ccataccaat gagcgctttc tggtcaattc gtggattgaa
      121 aacagcagcg gtgtaaaaga aaagtcattc atcattacac cgccactgtt tgttagtgaa
      181 cccaaaagcg aaaatacctt gcgtattatt tacaccggtc caccgctggc ggcagatcgt
      241 gagtctctgt tctggatgaa tgttaagacg atcccttcgg tagataaaaa tgcattgaac
      301 ggcaggaatg ttttgcaact ggcgatttta tcgcgcatga aattatttct ccgtccaatt
      361 caattacaag aattacccgc agaagcgccg gacacactca agttttcgcg atccggtaac
      421 tatatcaatg ttcataatcc atcacctttt tatgtcaccc tcgttaacct gcaagtgggc
      481 agccaaaagt tggggaatgc tatggcggca cccagagtta gttcacaaat tcccttaccc
      541 tcaggagtgc agggaaagct gaaatttcag accgttaatg attatggttc agtaaccccg
      601 gtcagagaag tgaacttaaa ctaaccgaat catctgacaa tatcagagct aattatgaaa
      661 atacccacta ctacggatat tccgcagagg tatacctggt gtctggccgg aatttgttat
      721 tcatctcttg ccattttacc ctccttttta agctatgcgg aaagttattt caacccggca
      781 tttttattag agaatggcac atccgttgct gatttatcgc gctttgagag aggtaatcat
      841 caacctgcgc gcgtgtatcg ggtggatctc tggcgtaatg atgagttcat tggttcacag
      901 gatatcgtat ttgaatcgac aacagaaaat acaggtgata aatcaggtgg gttaatgccc
      961 tgttttaacc aggtactcct tgaacgaatt ggccttaata gcagtgcatt tcccgagtta
     1021 gcccagcagc aaaacaataa atgcatcaat ttactgaaag ctgtacctga tgccacaatt
     1081 aactttgatt ttgcagcgat gcgcctgaac atcactattc ctcagataac gttgttgagt
     1141 agcgctcacg gttacattcc gcctgaagag tgggatgaag gtattcctgc tttactcctg
     1201 aattataatt tcaccggtaa cagaggtaat ggtaacgata gctatttttt tagtgagctc
     1261 agcgggatta atattggccc gtggcgttta cgcaacaatg gttactggaa ctattttcgc
     1321 ggaaatggat atcattcaga acagtggaat aatattggca cctgggtaca gcgcgccatt
     1381 attccgctga aaagtgaact ggtaatggga gacggcaata caggaagtga tattttcgat
     1441 ggtgttggat ttcgtggtgt acggctttat tcttccgata atatgtatcc tgatagccag
     1501 caagggtttg ccccaacggt acgtgggatt gcccgtacgg cggcccagct aacgattcgg
     1561 caaaatggtt ttattatcta tcaaagctat gtttcccccg gcgcttttga aattacagat
     1621 ttgcacccga catcttcaaa tggcgatctg gatgtcacca tcgacgagcg cgatggcaat
     1681 cagcagaatt acacaattcc gtattcaaca gtgccgattt tacaacgcga agggcgtttc
     1741 aaatttgacc tgacggcggg cgattttcgt agcggtaata gtcagcaatc atcacctttc
     1801 ttttttcagg gcacggcact cggcggttta ccacaggaat ttactgccta cggcgggacg
     1861 caattatctg ccaattacac cgccttttta ttaggactgg ggcgcaatct cggaaactgg
     1921 ggggcagtgt cgctggatgt gacccatgcg cgcagtcagt tagccgacga cagtcgtcat
     1981 gagggggatt ccattcgctt cctctatgcg aaatcgatga acaccttcgg caccaatttt
     2041 cagttaatgg gttaccgcta ttcgacacaa ggtttttata cccttgatga tgttgcgtat
     2101 cgtcgaatgg aggggtacga atatgattac gattacgacg gtgagcatcg ggatgaactg
     2161 ataatcgtga attaccacaa tttacgcttt agccgtaaag accgtttgca gttaaatatt
     2221 tcacaatcac ttaatgactt tggctcgctt tatatctctg gtacccatca aaaatactgg
     2281 aatacttcgg attcagatac ctggtatctg gtggggtata ccagcagctg ggttggcatc
     2341 agttattcac tctcattttc gtggaatgaa tctgtaggga tccccgataa cgaacgtatt
     2401 gtcggactta atgtttcagt gcctttcaat gttctgacca aacgtcgcta cacccgggaa
     2461 aatgcgctcg accgcgctta tgcctccttt aacgccaacc gtaacagcaa cgggcaaaat
     2521 agctggctgg ctggtgtagg tgggacctta ctggaaggcc acaacctgag ttatcacgta
     2581 agccagggtg atacctcgaa taatgggtat acgggcagcg ccacggcaaa ctggcaggcc
     2641 acttacggta cgctgggggt cgggtataac tacgaccgcg atcaacatga cgttaactgg
     2701 cagctgtctg gcggtgtggt cgggcacgaa aatggcataa cgctgagcca gcctttaggg
     2761 gataccaatg ttttgattaa agcgcctggc gcaggcggtg tacgcattga aaatcaaact
     2821 ggcattttaa ccgactggcg cggctatgcg gtgatgccgt atgccacggt ttatcggtat
     2881 aaccgtatcg cgcttgatac caatacgatg gggaattcca tcgatgttga aaaaaatatt
     2941 agcagcgttg tgccgacgca aggcgcgttg gttcgtgcca attttgatac ccgcataggc
     3001 gtgcgggcgc tcattaccgt tacccagggc ggaaaaccgg tgccgtttgg atcactggta
     3061 cgggaaaaca gtaccggaat aaccagtatg gtgggtgatg acgggcaagt ttatttaagt
     3121 ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa ctcacgctgc
     3181 attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt tatttcggca
     3241 gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg cagattatta
     3301 ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg tgcaaatagt
     3361 actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag caccaataac
     3421 cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt gtcagcaatt
     3481 tgtccacccg gcacgctggt gaattataca taccgtagtt atgtcaccaa ctttattgtt
     3541 ca
//
LOCUS       DAZFAH010000301         3533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFAH010000301 DAZFAH010000000
VERSION     DAZFAH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.303"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..908)
                     /locus_tag="ACHJWB_004668"
     CDS             complement(<1..908)
                     /locus_tag="ACHJWB_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4316146.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSVNC"
     gene            complement(992..1480)
                     /locus_tag="ACHJWB_004669"
     CDS             complement(992..1480)
                     /locus_tag="ACHJWB_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO4316147.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLVYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(1571..2527)
                     /locus_tag="ACHJWB_004670"
     CDS             complement(1571..2527)
                     /locus_tag="ACHJWB_004670"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4316148.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSSAKAKIVVDYLNVNNLNKVLRYYKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMADERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGEHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(2546..>3533)
                     /locus_tag="ACHJWB_004671"
     CDS             complement(2546..>3533)
                     /locus_tag="ACHJWB_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4316149.1"
                     /translation="LGSTGAFAANDRGFTSDTDWSEEQAGFNGHVGTTFEYETKSTDN
                     YLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQEEKGYHENETSLQNYISL
                     NKTFTIGNGFDFSLVNDLMYTDGNIDSPYVTGLHTVTVDNSFRPTLTYFNSDWNAGFY
                     SNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIEFFYQKKHNDEFNHGKIN
                     DRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSLQYNANRTYFKTIRKATL
                     GYEQNIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQDNIYVHAFYRF"
BASE COUNT         1010 a          745 c          643 g         1135 t
ORIGIN      
        1 ttgcaattca cactcaaaga gatatgttct ggacgaatac caaaacaaac gggtttatct
       61 atatacgctt tgagtactga ttgtttattc tcaggtatgt acaaccgaat atttttggca
      121 atgtcaatat agagtttacc gtcttcctca cgtacaaccg catctatcaa attcattgac
      181 ggggaaccaa taaagccagc aacgaatctg ttagcagggt ggttatacag attaactggt
      241 gtgtcgacct gcattattcg cccctgatta agcacacaaa tacgatcacc gagggtcaat
      301 gcttcaattt gatcgtgtgt aacataaatc atggttgctg ggtgaccctc atccttgaga
      361 gactgatgca actgagcaat tttgactcgc attgaaacac gaagtttcgc atcaaggtta
      421 gatagtggct catcaaataa gaaaacatct ggcttacgga caattgcacg gcctaccgcg
      481 acacgctgac gctgaccacc cgacatttct ttcggttttc ggtaaagtaa atcgctaatt
      541 tcaagttttt ctgcaacttc ttcgacaatt ttcttaatct cttcttttgg ccttttttgc
      601 atttttaaac caaaagccat attgtcaaat acagtcttat ggggatacag cgcatagttc
      661 tggaacacca ttgcaatgct gcgatcttta ggcggaaggg tattaacaag gcggtcgcta
      721 atgtaaattt caccacctga tacgtcttca agacctgcaa tcatgcgtaa tgtagttgat
      781 ttggcacacc cagatggtcc aacaaaaacc atgaattcac catcgtttat atcgagatca
      841 atgccgtgaa cagctttgaa tccattgtca taaatttttt caactttgcg tagaattacc
      901 gcggccataa tctgctctca ttaacaagaa ttttggaata ctgatactcc actattcaac
      961 gaatgttgaa tagtggagtg ctaaaaaata actatttaca tggtgctacc atggcggcta
     1021 ttgccttttt atcaggacca atttgaatcg tccaaacata ctttccatcg gcaggaatga
     1081 cgacttttgt atttttatcc atgcctcctt tcttgagtgt tttagcctga tttaatggca
     1141 attcaagacc tgttacagtg aactgcgggt tccagttcat agtcgcaaat tgcattgtta
     1201 ccgcacctgc tttttcagaa taaacaagct gataaatacc atctccctta taatgcattt
     1261 tacgctctgg cttcattaac caatagcctt ccgggagggt tccggaaatg taaagacccg
     1321 cagcaattgg ccctctttca tttacatcag ggaggttaca ttctgaatag cgaggatccg
     1381 ctttcacgac accttcctga ctgccagagg gtgaattgcc atcaacgcat cctgaaagaa
     1441 gaccagtcaa cacagccgca ataagcaatt tatttttcat ttaaactctc aataaaagta
     1501 gtatagctta gttaagctat ttctatgtca gtgttaatgt taatgtatat aaataatact
     1561 tctcaacgac tcactgttta tatacaccat gctgtttagt tagtttatta aggacagcga
     1621 gatttttgtt gtattctcgc gtatttttgg cagggtaacc aaaaaggtca ctcttttcag
     1681 gacaagaatg gcttacacct gatttagcct taattaccga ataactacca attgatatgt
     1741 gaccggcgat tccgacttga ccatgtgtaa taacatgttc gcctaaaaca acatgtcctg
     1801 agaaaccgca ttgtgaaaca atcaggcatt tattgccgat tatgcagtca tgtccaatct
     1861 gcacttgatt atcaatacga gtgccttgac caattatcgt atctccaagt gttcctcggt
     1921 caatggtgtt gttacaaccg atttcaacat catcacctat gataactcta ccgatgctat
     1981 cgactcggac atatgaatca cgttcatcag ccatatattc aaagctataa ttaccgatgg
     2041 aattattaga atcaataata acatcattac caataatagt gccttcttta atcactgtat
     2101 tcgcatgaat ggcaacatta tcacctatgg tcacacagtt cataatttta acacccggca
     2161 taaaatggca gttcatacct atctggcagt gtttaccaat gtatacatca ggtatcgttg
     2221 aagtgttttc ttgctcaaac aaacgatact tatgtacctt atagtatctt aaaactttgt
     2281 taaggttatt cacgttcaga taatcgacta caatctttgc cttagcgcta gaactaatga
     2341 tctcttccgg tccgataatt atatccgcac cagtcgttgg tatatgcaac aaatctccag
     2401 gtgtcataac aatcgagagt ccaccaacta aaggactatg gattggccga atggtatcaa
     2461 ccctgagtga tggatcacca tataaagttc cttgcatcaa cgttttaata tctagtgcgc
     2521 tgaacatagg tttccctctg tttaatcaaa agcgatagaa agcatgaaca taaatattat
     2581 cctgattgag cacattatat ttttcttcat ctcttggatc ataaaagttt ttgtgctctt
     2641 tttcccaggc atgtttatat tcaatcttgg ctatccagtc gtcacctatg ttttgttcat
     2701 aacctagagt ggcttttcga attgttttga aataggttct gtttgcattg tattgcaaac
     2761 tatctgcgtt ttctgttttc ccgtgtccga agcgggtgcg tagatacaga ttacctgcgt
     2821 tttcaaaagc atatttgata attggctcgt agtaagtttc ggtataaata ctacgatcat
     2881 ttattttacc gtgattaaat tcatcgttat gctttttctg gtagaaaaat tcaataccaa
     2941 actcccatgc accaaaacgt ttatagggct gaaatagtat actttgacct tcttcttcgc
     3001 gtacgcccca ggagctctta tcatttcgac tgtataagta ttcaacgttt gaataaaaac
     3061 ctgcgttcca gtcagagtta aagtacgtca acgtagggcg aaaactgttg tcaacagtta
     3121 cagtatgcaa gcctgtaaca taagggctat ctatatttcc gtcggtatac atcaggtcgt
     3181 ttaccagact gaagtcgaaa ccgttaccaa tagtgaacgt tttgtttagt gatatatagt
     3241 tttgcagact tgtttcattt tcgtgatagc ctttttcttc ttgtcggcga tcgatagact
     3301 taaggccata gattgctgca aattgccaat taggttggct gaaataggcc tggaaaatct
     3361 cgtgtgtttt agtatattcc tttactgttt tacctaaata gttatcagtg cttttggtct
     3421 catattcaaa tgtagtacca acatgtccat taaagcccgc ttgttcttct gaccagtcag
     3481 tgtcggaggt aaagccgcga tcgtttgccg caaaggctcc tgttgagcct aaa
//
LOCUS       DAZFAH010000302         3463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFAH010000302 DAZFAH010000000
VERSION     DAZFAH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.304"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2633
                     /gene="eae"
                     /locus_tag="ACHJWB_004672"
     CDS             <1..2633
                     /gene="eae"
                     /locus_tag="ACHJWB_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin"
                     /protein_id="HFO4316150.1"
                     /translation="LFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAP
                     GQQIILPLKKLPFEYSALPLLGSVPLVAAGGVAGHTNKLTKISPDVTKSNMTDDKAFN
                     YAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNN
                     FDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNIFIDQ
                     DFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYL
                     PSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTG
                     NENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDIL
                     SLNIPHDINGTERSTQKIQLIVKSKYGLDRVVWDDSSLRSQGGQIQHSGSQSAQDYQA
                     ILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTADKTSA
                     KADNADTITYTAVVKKNGVTQANVPVSFNIVSGTATLSAKSANTNSSGKVTVTLKSDK
                     PGQVVVSAKTAEMTSALNAHAVIFVDQTQASITEINADKKTAKANGSDAITYTVKVMK
                     DGKPLSAQDVTFTATLGTLSKSTEKTDANGYAKVTLTSKTTGKSLVSARISGSAIDVK
                     APEVEFFTPLAIDDGNVEIVGTGIKGTLPTVWLQYGQVKLKASGGDGKYTWSSANTGI
                     ASVDSTGQVTLRDKGSTTITVVSGDKQTATYIIARPSSMIVSINERMTYNNAMSSCQS
                     LSGRLPSSQKELADVFDTWGAANKYEHYETRNAMISWIKQTDQDMRQGVASTYDLVKK
                     NPLTNKVDINKPNAYATCVK"
     gene            complement(2847..>3463)
                     /gene="escD"
                     /locus_tag="ACHJWB_004673"
     CDS             complement(2847..>3463)
                     /gene="escD"
                     /locus_tag="ACHJWB_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4316151.1"
                     /translation="YYNSIICNNQIISAINDILTEYGYKDIIITKGNKPGFFLLSGYI
                     PPSPKWSEVENLLLNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVII
                     VAGEVSQQNESKILAIINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMTNPTI
                     ALDNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1004 a          664 c          785 g         1010 t
ORIGIN      
        1 gcctttttta tacgttgaaa acaggtgaaa ctgttgccga tctttctaaa tcgcaagata
       61 ttaatttatc gacgatttgg tcgttgaata agcatttata cagttctgaa agcgaaatga
      121 tgaaggctgc gcctggtcag cagatcattt tgccactcaa aaaacttccc tttgaataca
      181 gtgcactacc acttttaggt tcggtacctc ttgttgctgc aggcggtgtt gctggtcaca
      241 cgaataaact gactaaaata tccccggacg tgaccaaaag caacatgacc gatgacaagg
      301 cattcaatta tgcggcacaa caggcggcga gtctcggtag ccagcttcag tcgcgctctc
      361 tgaacggcga ttacgcgaaa gataccgctc ttggtatcgc tggtaaccag gcttcgtcac
      421 agttgcaggc ttggttacaa cattatggaa cggcagaggt taatctgcag agtggtaata
      481 actttgacgg tagttcactg gacttcttat taccgttcta tgattccgaa aaaatgctgg
      541 catttggtca ggtcggagcg cgttacattg actcccgctt tacggcaaat ttaggtgcgg
      601 gtcagcgttt tttccttcct gcaaacatgt tgggctataa catcttcatt gatcaggatt
      661 tttctggtga taatacccgt ttaggtattg gtggcgaata ctggcgagac tatttcaaaa
      721 gtagcgttaa cggctatttc cgcatgagcg gctggcatga gtcatacaat aagaaagact
      781 atgatgagcg cccagcaaat ggcttcgata tccgttttaa tggctatctg ccatcatacc
      841 cggcattagg tgccaagctg atgtatgagc agtattatgg tgataatgtt gctttgttta
      901 attctgataa gctgcagtcg aatcctggtg cggcgaccgt tggtgtaaac tatactccga
      961 ttcctctggt gacgatgggg atcgattacc gtcatggtac gggtaatgaa aatgatctcc
     1021 tttactcaat gcagttccgt tatcagtttg ataaaccgtg gtctcagcaa attgagccac
     1081 agtatgttaa cgagttaaga acattatcag gcagccgtta cgatctggtt cagcgtaata
     1141 acaatattat tctggagtac aaaaagcagg atattctttc tctgaatatt ccgcatgata
     1201 ttaatggtac tgaacgcagt acgcagaaga ttcaattgat cgttaagagc aaatacggtc
     1261 tggatcgtgt cgtctgggat gatagttcat tacgtagcca gggcggccag attcagcata
     1321 gcggaagcca aagcgcacaa gattaccagg ctattttgcc tgcttatgtg caaggtggta
     1381 gcaatattta taaagtgacg gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg
     1441 tacagcttac tattaccgtt ctgtcgaatg gtcaggtggt cgaccaggtt ggggtaacgg
     1501 actttacggc tgataagact tcggctaaag cggataacgc cgataccatt acctataccg
     1561 cggtggttaa aaagaatggg gtgactcagg ctaatgtccc tgtttcattt aatattgttt
     1621 caggaactgc aactttaagt gccaagagtg ccaatactaa tagtagtggt aaggtgactg
     1681 taacgctgaa atcagataag ccgggtcagg tagttgtgtc tgccaaaacc gcggagatga
     1741 cttcagcact taatgcccat gcggttatat ttgttgatca aactcaggcc agtattactg
     1801 agattaatgc tgataaaaaa acagcgaagg caaatggatc tgatgcgatt acctatactg
     1861 ttaaagtgat gaaagacggt aagccgttat ctgcccagga tgtgaccttt acggcaacct
     1921 taggtacgtt aagtaagtcc actgaaaaaa cggatgcgaa tggctatgct aaagtaacat
     1981 taacatcgaa gactacagga aaatcgcttg ttagcgctcg tattagcggg agcgcaattg
     2041 atgtcaaagc acctgaagtt gaattcttca ccccacttgc tattgatgac ggtaatgttg
     2101 aaattgtagg aactggaatc aaagggacgt tacctacggt atggttgcaa tatggacaag
     2161 ttaagttgaa agccagtgga ggtgatggga aatatacatg gagttcggca aatactggaa
     2221 ttgcttcggt ggattccact ggtcaagtta ccttaaggga taaggggagt acaactatta
     2281 ccgttgtatc aggtgataag caaactgcaa cttatataat tgcaagacct agcagtatga
     2341 ttgtctctat aaacgagcgt atgacttata ataacgcaat gagttcatgc cagagcttga
     2401 gcgggaggtt gccatcttct caaaaagaac tagctgatgt attcgataca tggggggcag
     2461 caaataaata tgaacattat gagactcgta acgccatgat atcgtggata aaacaaacag
     2521 atcaggatat gaggcaggga gttgcaagta catacgatct ggttaagaaa aatccgctta
     2581 ctaataaagt agatataaat aagcctaatg cttatgcaac ttgcgtaaaa taaactcaat
     2641 aaacatctaa tagatgtccg gctaagatgt atgttttagt cggacatttt tatgcgatat
     2701 attctatatt agtacctttc gattagtatg ctggtaagta atcctccagc caccttttct
     2761 cttcgcccaa taagctgaca ttatggagac catatctgca tccacacaat aaaaaaccct
     2821 ccgaagaggg ggaagatgac aagacgttaa tacgatagcg gaatatgtat atattcgtct
     2881 gggcgggaga tgttgatacc atctttagga tcaatcgcgt ctatgacata gcctcctttc
     2941 agaatgctac caattcccag gcttgtaccg ttatcaagag caattgttgg attcgtcatc
     3001 gtgccgctga ttctgagtat ttttccagga aagatatctg ctgaaatgta cggttgaata
     3061 ttctgaaata gtatcttgac gttactattt ttgttcattg catttataat ggcaaggatc
     3121 ttactctcgt tctgttgaga aacttccccc gcaacaatga tgacatcatt ctttttgaaa
     3181 atattgacat agtttattaa tttattcttc ttaaactctg atgccagttc attgatttta
     3241 ttgttactgt tgttgtgtat ttcccatcca gcaactcctg gcgtattcag taacagattt
     3301 tcaacttcgc tccatttggg tgaaggggga atgtatcccg ataacagaaa gaatccaggc
     3361 ttattacctt ttgtaatgat gatatcttta taaccgtatt ccgttaaaat atcattaatt
     3421 gcggatatga tttggttatt gcaaatgatg ctattgtaat aaa
//
LOCUS       DAZFAH010000303         3439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFAH010000303 DAZFAH010000000
VERSION     DAZFAH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.305"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="ACHJWB_004674"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="ACHJWB_004674"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(298..825)
                     /gene="mobB"
                     /locus_tag="ACHJWB_004675"
     CDS             complement(298..825)
                     /gene="mobB"
                     /locus_tag="ACHJWB_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4316152.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(807..1391)
                     /gene="mobA"
                     /locus_tag="ACHJWB_004676"
     CDS             complement(807..1391)
                     /gene="mobA"
                     /locus_tag="ACHJWB_004676"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4316153.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDTPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1461..1730
                     /locus_tag="ACHJWB_004677"
     CDS             1461..1730
                     /locus_tag="ACHJWB_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4316154.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1807..2793
                     /gene="srkA"
                     /locus_tag="ACHJWB_004678"
     CDS             1807..2793
                     /gene="srkA"
                     /locus_tag="ACHJWB_004678"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4316155.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2810..3436
                     /gene="dsbA"
                     /locus_tag="ACHJWB_004679"
     CDS             2810..3436
                     /gene="dsbA"
                     /locus_tag="ACHJWB_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4316156.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          870 a          825 c          849 g          895 t
ORIGIN      
        1 tgttttggcg gattgagaga agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt tgcctggcgg ccttagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtttc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc gaaaggccat ccgtcaggat ggcctttttg
      241 cgttggtgaa aaaatgccgg gtgcgacgct ggcgcgtctt atccagccta ctcttgctta
      301 tccgttctgc ttttgcatcc actccaccac aaaatcagcc agcccctcaa catcattaat
      361 atccagtaat gcgacatcaa gattaagcgg cacatcactg gctacagcaa taacatgcct
      421 gtctatcact aattcttcag gtcgatgtcc ggctccatcg cgaaacagca caatctttgc
      481 gatctcttca tgcttaaacc cttcgaccag aatcaaatcc agctttgagg tatccattcg
      541 acttgcgaga aactgtagat ccagctcttc ttcgtctggt gtttccgtca tcaaggccca
      601 tcgttgctgg ctggcaacga tggtttgtgc cgcgccagcc ttgcgcagct catagctatc
      661 tttgcctggc ttatcaacat ccatatcatg gtgcgtatgc ttaatcagcc ctggacggat
      721 ccctctggcg cataatgccg ggatcagttg tttcaaaagc gtagtttttc cggtaccact
      781 ccacgcggca aaggcgagta acggtatcat cgtttttcct gccatcgggc aagctcctct
      841 ggcgtattca cgttaacaaa tgcatcttta tgatcgctga aatcaaccgc atgaccgcca
      901 gccagacgca taaatgccat tacccggcgt tctcctgctt gcagatattc cagtaataaa
      961 ggctcaattg cgcggtttac cagagcaata gtcgggtgat cgcgttcacc gtcgtggacc
     1021 cacacgacag gcgtatcttt gcgctgatga ttaagccggg ctgctaaatc ctcgggaata
     1081 taaggagtat cgcacggaca aaacaaaaac cactcgcctg cttcctgctg cattactgaa
     1141 agcattcctg ccagagggcc tgggtaatcc gccagtgaat cttcaatcac tttcagaccg
     1201 cttgcctgat agatttcctg atgacgatta gcattaacca cgacgtgaga gagctgcgcc
     1261 ataagcgcgt cagcgacatg ttgccataat ggtttgccgt ttaattccag caatccttta
     1321 tctacgccgc ccattcgtct ggctttaccg cctgccagca caacgcctgt tatcgtcgtc
     1381 atcagattca ccgatatcgc ctcttttatt gtgggattga ccctgctaac gtgtctgtct
     1441 caagagtaag gagcattact atgaaatgta aacgtctgaa tgaagttatt gaactcctcc
     1501 agccagcctg gcaaaaagag ccagacctta acctgttgca atttttgcag aaactggcga
     1561 aagagtcagg ttttgacggc gaactggcgg atttgacgga tgacattctg atctatcacc
     1621 tgaaaatgcg tgattccgcg aaagatgcgg tgatcccggg tttgcagaaa gattatgaag
     1681 aagacttcaa aacggcgctg ttacgcgctc gcggcgtaat taaagagtaa aagcttgtaa
     1741 gcggtgccac caaaatcatc gtgaaatgat atccttcatc attcgtaatg ttttccggat
     1801 gatgggatga acaacagcgc ttttactttc cagacactac acccggatac catcatggat
     1861 gctctatttg agcaagggat ccgggtggat tccggtctta ccccgcttaa cagctatgaa
     1921 aaccgtgtct atcaatttca ggacgaagat cgtcgacgtt ttgtcgtcaa attttatcgc
     1981 cctgaacgtt ggacagccga tcaaatcctc gaagaacatc aatttgcgtt gcagttggta
     2041 aatgatgaag ttccggtcgc agcacctgtg gcctttaacg gtcagacttt attgaatcat
     2101 cagggatttt atttcgctgt ttttccaagc gtcggtggtc gccagttcga agctgataat
     2161 atcgatcaga tggaagcggt tgggcgttat ttagggcgta tgcaccagac ggggcgcaaa
     2221 cagcttttta tccatcgccc gaccatcggt ctgaatgaat atctcattga gccacgcaag
     2281 ctgtttgagg acgctacact gataccttcc gggttgaaag cggcattcct gaaagcgaca
     2341 gatgagctga tcgccgccgt tacagcgcac tggcgggaag atttcaccgt tctgcggcta
     2401 catggagact gccacgccgg gaatattctc tggcgcgatg gtccaatgtt tgttgatctg
     2461 gatgatgcac gtaatggtcc agctattcag gatttgtgga tgttgctcaa tggcgataaa
     2521 gccgagcagc ggatgcaact ggaaactatt attgaagctt atgaagaatt tagcgagttc
     2581 gacaccgctg aaatcggact gattgaacct ttacgcgcca tgcgtttggt ttattatctt
     2641 gcctggctaa tgcggcgttg ggctgatccc gcgttcccga aaaatttccc gtggttaacc
     2701 ggggaagatt actggctgcg acaaacggcg acttttatag aacaggcaaa agttctacaa
     2761 gaaccccctt tacaattaac acctatgtat taatcggaga gagtagatca tgaaaaagat
     2821 ttggctggcg ctggctggtt tagttttagc gtttagcgca tcggcggcgc agtatgaaga
     2881 tggtaaacag tacactaccc tggaaaaacc ggtagctggc gcgccgcaag tgctggagtt
     2941 tttctctttc ttctgtccgc actgctatca gtttgaagaa gttctgcata tttctgataa
     3001 tgtgaagaaa aaactgccgg aaggcgtgaa gatgactaaa taccacgtca acttcatggg
     3061 tggtgacctg ggcaaagatc tgactcaggc atgggctgtg gcgatggcgc tgggcgtgga
     3121 agacaaagtc actgttccgc tgtttgaagg cgtacagaaa acccagacca ttcgttcagc
     3181 atctgatatc cgcgatgtat ttatcaacgc aggtattaaa ggtgaagagt acgacgcggc
     3241 gtggaacagc ttcgtggtca aatctctggt cgctcagcag gaaaaagctg cagctgacgt
     3301 gcaattgcgt ggcgttccgg cgatgtttgt taacggtaaa tatcagctga atccgcaggg
     3361 tatggatact agcaatatgg atgtttttgt tcagcagtat gctgatacag tgaaatatct
     3421 gtccgagaaa aaataattc
//
LOCUS       DAZFAH010000304         3318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFAH010000304 DAZFAH010000000
VERSION     DAZFAH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.306"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..209
                     /locus_tag="ACHJWB_004680"
     CDS             <1..209
                     /locus_tag="ACHJWB_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316157.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKREQQQANAEPEEQVDPRK
                     AAVAAAIARAQAKKAAQQKVVNED"
     gene            210..1268
                     /gene="rsxD"
                     /locus_tag="ACHJWB_004681"
     CDS             210..1268
                     /gene="rsxD"
                     /locus_tag="ACHJWB_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4316158.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1272..1892
                     /gene="rsxG"
                     /locus_tag="ACHJWB_004682"
     CDS             1272..1892
                     /gene="rsxG"
                     /locus_tag="ACHJWB_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4316159.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1896..2591
                     /gene="rsxE"
                     /locus_tag="ACHJWB_004683"
     CDS             1896..2591
                     /gene="rsxE"
                     /locus_tag="ACHJWB_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4316160.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFFGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2591..3226
                     /gene="nth"
                     /locus_tag="ACHJWB_004684"
     CDS             2591..3226
                     /gene="nth"
                     /locus_tag="ACHJWB_004684"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4316161.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQYNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          778 a          845 c          882 g          813 t
ORIGIN      
        1 ctaatgcgga accagaagaa caggttgatc cgcgcaaagc cgccgtcgaa gccgctattg
       61 cccgagccaa agcgcgcaaa cgggaacagc aacaggctaa tgcggaacca gaagaacagg
      121 ttgatccgcg caaagcggca gttgccgcgg ctattgcccg cgctcaggcc aaaaaagccg
      181 cccagcagaa ggttgtaaac gaggactaaa tggtattcag aatagctagc tccccttata
      241 cccataacca gcgccagaca tcgcgcatta tgctgttggt gttgctcgca gccgtgccag
      301 gaatcgcagc gcaactgtgg ttttttggtt ggggtactct cgttcagatc ctgttggcat
      361 cggttagtgc tctgttagcc gaagctctcg tactcaaact acgcaagcag tcggtagccg
      421 caacgttgaa agataactca gcattgctga caggcttatt gctggcggta agtattcccc
      481 ccctcgcgcc atggtggatg gtcgtgctgg gtacggtgtt tgcggtgatc atcgctaaac
      541 agttgtatgg cggtctgggg caaaacccgt ttaatccggc aatgattggt tatgtggtct
      601 tactgatctc cttccctgtg cagatgacca gctggttacc gccacatgaa attgcggtca
      661 acatcccagg ttttatcgac gccatacagg ttattttcag cggacatacc gccagtggtg
      721 gtgatatgaa cacactacgc ttaggtattg atggcattag tcaggcgaca ccgctggata
      781 catttaaaac ctctgtccgt gccggtcatt cggttgaaca gattatgcaa tatccgatct
      841 acagcggtat tctggcgggc gctggttggc aatgggtaaa tctcgcctgg ctggctggcg
      901 gcctgtggtt gctatggcag aaagcgattc gctggcatat tcccctcagc ttcttagtaa
      961 cgctggcgtt atgcgcaacg ttgggctggt tgttctcacc agaaacactg gcagcaccgc
     1021 aaattcatct gctgtctggt gcgaccatgc tcggcgcatt ctttattttg actgacccgg
     1081 ttaccgcttc tacgaccaat cgtggtcgtc ttattttcgg cgcgctggcg ggcttattag
     1141 tctggttgat ccgcagtttc ggcggctatc ctgacggcgt ggcttttgcc gtcctgctgg
     1201 cgaacatcac ggttcctctg atcgattact acacgcgtcc gcgcgtctac ggccatcgca
     1261 aagggtaaac catgctgaaa actatccgaa aacacggcat tacgttggcg ctatttgcag
     1321 cgggttcaac agggttaact gcggccatca accagatgac caaaacgacg attgctgaac
     1381 aggccagtct gcaacaaaag gcgttatttg atcaggtgct gccagccgaa cgctataaca
     1441 atgcgctggc acagagttgc tatctggtaa ctgcgccaga gttaggtaaa ggtgagcatc
     1501 gggtttacat cgccaaacag gatgacaaac cggtagccgc cgttctggaa gcaaccgcgc
     1561 cagatggcta ttccggtgcg attcagctgc tggtgggagc cgattttaac ggcacggtac
     1621 ttggcacgcg cgtgacagag caccacgaaa cgccagggct tggcgataaa atcgaactgc
     1681 gcctttctga ctggatcacc cattttgcgg gtaaaaaaat cagtggtgca gatgatgcgc
     1741 actgggcggt gaagaaagat ggtggtgatt tcgaccagtt caccggcgcg acgattactc
     1801 cccgcgcggt ggttaatgcg gtaaaacgcg ccggattgta cgctcagacg ttaccggcac
     1861 aactttctca acttcctgcc tgtggagaat aaagcgtgag cgaaattaaa gacgttattg
     1921 ttcaggggtt gtggaaaaac aactctgcgc tggtccagct gctcggcctt tgtcctctgt
     1981 tggcggtcac gtccactgcc actaacgctc tgggtttagg acttgcgact acgctggtac
     2041 tgacgctgac caacctgacc atttcgacgc tgcgtcactg gacgccagcc gagatccgca
     2101 ttcccattta cgtgatgatc atcgcctcgg tggtcagcgc tgtacagatg ctgatcaacg
     2161 cctacgcctt tggcctgtat caatcattag ggatttttat tccgctgatt gtcactaact
     2221 gtatcgttgt gggccgcgct gaagccttcg ccgccaaaaa aggtccggcg ctttcggcac
     2281 tggacggctt ttcaattggt atgggcgcaa cctgcgccat gttcgtgctg ggttcactac
     2341 gcgaaattat cggcaatggc acattgtttg acggtgcaga tgcgctgtta ggtagctggg
     2401 caaaagtatt acgcgtggaa attttccaca ccgactcccc tttcctgctg gcgatgctgc
     2461 ctccaggtgc attttttggc ctgggactga tgctggcagg aaaatacctg attgatgaaa
     2521 gaatgaaaaa gcgccgtact gaagcagctg ccgaacgtgc attgccaaac ggtgaaacag
     2581 ggaatgtctg atgaacaaag caaaacgcct ggagatcctc actcgcctgc gtgagaacaa
     2641 tcctcatccc accaccgagc ttaatttcag ttcgcctttt gaattgctga ttgccgtact
     2701 gctttccgct caggcgaccg atgtcagtgt taataaggcg acggcgaaac tttacccggt
     2761 ggcgaatacg cctgcagcga tgcttgaact gggcgttgaa ggggttaaaa cctatatcaa
     2821 aacgattggg ctttataaca gcaaagcaga aaatatcatc aaaacctgcc gtatcttact
     2881 ggagcagtat aatggcgagg ttccggaaga tcgtgctgcg cttgaagccc tgcccggcgt
     2941 aggtcgtaaa acagccaacg tcgtattaaa cactgcattc ggctggccga ctattgctgt
     3001 cgacacgcac attttccgcg tttgtaatcg tactcaattt gcgccgggga aaaacgtcga
     3061 acaggtagaa gaaaagctac tgaaagtggt tccggcagag tttaaagtcg actgccacca
     3121 ttggttgatc ctgcacgggc gttatacctg cattgcccgc aagccccgct gtggctcttg
     3181 tattattgaa gatctttgtg aatacaaaga gaaagttgac atctgaagaa aaggggtaac
     3241 accgattacc ccattgataa cctttcttta tcctctttta aaacatcttt ttaacgtgaa
     3301 tgatgccatt gcttagcg
//
LOCUS       DAZFAH010000305         3305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFAH010000305 DAZFAH010000000
VERSION     DAZFAH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.307"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..453)
                     /locus_tag="ACHJWB_004685"
     CDS             complement(<1..453)
                     /locus_tag="ACHJWB_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO4316162.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAA"
     gene            complement(473..934)
                     /locus_tag="ACHJWB_004686"
     CDS             complement(473..934)
                     /locus_tag="ACHJWB_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4316163.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(962..2863)
                     /locus_tag="ACHJWB_004687"
     CDS             complement(962..2863)
                     /locus_tag="ACHJWB_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4316164.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IMGGSVFIN"
BASE COUNT          705 a          927 c          838 g          835 t
ORIGIN      
        1 ggcagcgggc tttccgttaa tgtttacatt ttcagaaccg gtattgatgg cgccgtccgt
       61 gtcacaaaag ctgtttatcc atgattcaag ctggttctgt ttctcctcca cataatcact
      121 cgccttatag ctggcataca tcagcgcagg caccagaacc agcgcataag ggccggtggc
      181 acaggcggcc ataatcaggc aaccttctgc gatggtcact atcgccccca gcacggcccc
      241 tgccagcgcc cccaaaaaac tcttgtgctg aatttcatcg cccagcctgg cggcgggggg
      301 gccggacttt tttgatgcca gcatctgtgc aatggctttg tcgttggctg cccgtttcat
      361 ctggctggcg accatcgcct gctgtgacag cattcgcatt gccagcgtac cgcctgcggt
      421 cgctccctgt ggcccgcctg gtccttcact catgtgttat ccctcgttta tgtcagcgcg
      481 gctggtcctt ttggatgtca ttctccggtg gcaacggggt gaagctcttt atcacctcca
      541 gcagcatcgt cttttgactg ttcttcaggg ataaggaggt gctgaaagtg aaggtcagca
      601 gcgtatctcc acgccgctgg aatacggcta tctggctaat ccgcccctga ttcccctcat
      661 cttccggacc gttccaggaa taatccagca gccaggccgg ggcctctgcc agagtgattt
      721 cccggcaggc atgttcggtg tacccatcaa gtttgccctg gtacagcgcc agcgtttcct
      781 ggtacagctc tggtgtcagg gttccctgtg gaacggcttc gcgggtaatg accaggttga
      841 tgccactgtt atctgtggac accaggacct gcattgactg atcctgccat tgagacggca
      901 gttgaatgtg gccttcatta aaagtgaact gcataaaata ctccctgata atctgagtga
      961 atcagttgat gaaaacagaa ccgcccatga tggtgatgcc atccttcgac agcctgatta
     1021 ccgtcgattc cccgacctgt agctgaatgc catccggcgt cagcgcaatt ttgctttcct
     1081 ccacggccag caaaatttcc ttaatcgccg tataagcctg tataccgtcg ccttcgccac
     1141 cattgtttac agcgtcaaca tgcaccgaac cggcaatcgt gcctctgtcc tgaaagacat
     1201 cctcagataa agaccctttc agtactccgg tgatcttgtg gcctttttcc accgtaagaa
     1261 ggcggttacc ttcctggatc gtgaccgtct ggtcattttt tacggtttcg cggtgatcgt
     1321 gttttacggt ggtggtacgg tcgttgagca cttcggtatc catgttcttc tgcgcgtgga
     1381 tgtagacctg ttctctccct gtcgcatcat caaacttcag ctcattaaat ccgctgccca
     1441 tgtacgtttt ggagcggatg gtcatctgcg tctttgttcc cggcaggctg ccgggggtgc
     1501 ggttttcctg gtggtaggtg cgccccataa tgatcggctg gtccggatcg ccgttgagga
     1561 agtctacaat cacctcctga cccacacgcg gtatcgccag gtggccaaaa ccggtgcctg
     1621 cccacgcctg tgccacacgg atccagcacg aactgtcttg gtctgccggg ttataacggt
     1681 cccagttgaa cttcacccgc acgcgaccat gttcgtcgca gaagatttcc tcgcctgccg
     1741 gtcctgtcac gacagcgctc tgcgggccgt cgaccagcgg tttcagcaac ggctgtggtc
     1801 gccatgttct gtctgccggg atcaccgcaa aatggttctc cagcgccgtc cccgctccct
     1861 gccgtcctgg caccgcctgt ggctgttcgc cgtgcagttc acttgccacc acctgccatt
     1921 cccggttcag gttcgcctgc ggatgccccg tcagcacaat tcgccgtccc ggccatatct
     1981 ccggcgagcg gctcattccc cgcgcggttt ctgcgttgtt tcgccagccg tccatctgcc
     2041 agcgggcaaa gttctgcccg tgggcgctct tgaaacgtcc ggggtagtca tacacttcat
     2101 actgcgtgcg ctggtagtcc tggtgctggc cttcctgttc aaaacgtccg gcccagccgg
     2161 ggcgtttaaa ggtgtagtct ttggtcacca cggaagaagg gcggatttgt gcgctgtagc
     2221 ggaactggct gatgcagagg gtgctcacct cggtacgggt gttcgggttc catgggattt
     2281 caaaagattc gggcagatgg cggactgtgt cgcacagcac caggctctgg tcggtacttt
     2341 tgtaagcatg ctcctcataa aagaagatgc cttcctccgc cgccatccgg cacaggaaat
     2401 cgtaatcagt ctcaccgtac tggacacaaa actcacggga aggatgcggc tcgctgaaca
     2461 gcggactcca ttcggtcacc ccgttttcct gcaacatcgt gccgaggatg cttttgatgt
     2521 cctcgttctg gaagatacgg aagttctggc gcagaccggc acgccacagc ggcgggtgca
     2581 ccttcatgct gtacagcatc tggtttttgt cgttctcccc gagttcaaac caggtcacca
     2641 cgcctttcac ccggcgctgt acgtcatcgc cctgccatat cgtcaggtag gccattttgt
     2701 ccagcacctg cgcaaattca agggagagaa actgctggct gaccagggag agatcgaggg
     2761 aaaagagcga agagagtgac tggttcagat gaaaggaaac caccgcaaaa gcgtccggcg
     2821 gcaggccgtc cacttccagt gtgaaacgta atccggttga catagcaacc tccagtaaaa
     2881 tgaataatga tccgtaccgt acaactgcgg ataatgtgat aagcgggacg acttcagacg
     2941 ttattaggga aggaacagaa aatgctatgg ttatgtgaga attttgttta aatattactt
     3001 tacagaaacg ggtgtctgtt cagtcaaaaa aataatcaca agctcaacct cttgtttctt
     3061 ggcgttattc aggacaggta cgcatatcac aacgagtaac aaaaacgcaa agttaaacca
     3121 atattcataa tatattttta ttcacagcaa taaataatcc aattaacatt attaaaatga
     3181 atgattgcat aagaaatata tgctaccgct ccaccctgat aacatgatct gcacattgca
     3241 catcttaacg ttgttaaata ctggctcaat agtggatatt ttaaattagg aatttatcaa
     3301 attca
//
LOCUS       DAZFAH010000306         3298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFAH010000306 DAZFAH010000000
VERSION     DAZFAH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.308"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..1680
                     /locus_tag="ACHJWB_004688"
                     /pseudo
     CDS             160..1680
                     /locus_tag="ACHJWB_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(1705..2043)
                     /gene="maoP"
                     /locus_tag="ACHJWB_004689"
     CDS             complement(1705..2043)
                     /gene="maoP"
                     /locus_tag="ACHJWB_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4316165.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2162..3001
                     /gene="hdfR"
                     /locus_tag="ACHJWB_004690"
     CDS             2162..3001
                     /gene="hdfR"
                     /locus_tag="ACHJWB_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4316166.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3097..3172)
                     /locus_tag="ACHJWB_004691"
     tRNA            complement(3097..3172)
                     /locus_tag="ACHJWB_004691"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3137..3139),aa:Trp,seq:cca)
     gene            complement(3181..3257)
                     /locus_tag="ACHJWB_004692"
     tRNA            complement(3181..3257)
                     /locus_tag="ACHJWB_004692"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3221..3223),aa:Asp,seq:gtc)
BASE COUNT          885 a          813 c          808 g          792 t
ORIGIN      
        1 ttcatccttt gtccgctgca tttttgcgag catcattcca ccgccagaac aactcaactt
       61 cacttgcaca aataaacagg aatcagccac taaaattcac gaattagcgg gctacagcag
      121 cgatatctta tgcacataat ggtgactcaa ggagggctta tgtcactgtc aattgttcat
      181 acccgcgcag ccctgggagt aaatgcgccc acgatcactg ttgaggtaca tatcagtaaa
      241 ggtctacccg gcttaacgat ggtgggctta ccagaaacaa cggtaaaaga agctcgcgat
      301 cgcgtgcgca gcgccattat caatagcgga tatgaatatc cggcgaaaaa aatcaccatc
      361 aacctggctc cagctgatct gccaaaagaa gggggacgat atgatttacc tatcgccatt
      421 gcgttgctgg cggcctcaga acagcttaca gccaataagt tagatgaata tgaattagtc
      481 ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg gcgcaatctc cagtgcaact
      541 gaagctatta agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg
      601 ctaattaacg gtgaaggatg cctgatagcc gatcatctgc aagctgtctg tgcgtttctg
      661 gaaggtaagc acgctctcga acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa
      721 catgatctca gtgatgttgt cggtcaggaa caaggaaagc gaggactgga aattaccgcc
      781 gctggcgggc acaacctttt actgattggg ccgccgggaa caggtaaaac aatgctcgcc
      841 agccgtatta atggcctgtt gccagattta agcaatgaag aggcactgga gagtgctgcg
      901 atattaagtc tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg cccgttccgc
      961 tcacctcatc acagtgcatc gttaactgcg atggtaggcg gtggcgcaat tccagggccc
     1021 ggtgaaattt cgctggcgca taacggcgtg ctttttcttg atgagttacc tgaatttgaa
     1081 aggcgtacac tggatgcctt gcgagagccg attgaatccg ggcagatcca tctttcacgc
     1141 acgcgagcaa aaataaccta tccagcccgt ttccagcttg tcgcggcgat gaatcccagc
     1201 cctaccggac attatcaggg aaaccataac cgctgcacac cagagcaaac gctgcgttat
     1261 ctcaaccgac tctctggtcc ctttctcgac cgcttcgatc tctcactgga gatcccatta
     1321 ccaccccccg gcattttgag taaaacggta gtgccgggag aaagcagcgc caccgttaaa
     1381 caacgcgtaa tggccgccag agagcgccaa tttaagcggc agaataaact gaatgcctag
     1441 ctggatagtc cggaaatacg ccaattctgc aaacttgaga gcgaagatgc gatgtggttg
     1501 gaaggaacac tgatccatct ggggttatcg attcgtgcct ggcagcggtt attgaaagtt
     1561 gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcagca tttgcaggag
     1621 gcagttagct atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact actgacataa
     1681 aaaaagggca tttcgccctt tttattaatc atcagaatcg gtgtagtctt cagcaccttc
     1741 aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat
     1801 atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc
     1861 tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa
     1921 ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga
     1981 gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc
     2041 catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc
     2101 ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac
     2161 tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg
     2221 agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga
     2281 aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc
     2341 cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgtaa
     2401 ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg cctcgttgtg
     2461 ggaatgtatg cttaatcagt ggctgggacg tttgtatcaa aatcaggatg cccatacagg
     2521 cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg
     2581 ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt
     2641 gctgggatat ttcactttag cgctttatac cagtgcccct tcaaaactaa agggagatct
     2701 taattatctg cgacttgagt gggggccaga ttttcaacag catgaggcag gtttgatcgg
     2761 tgctgacgaa gtgcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc
     2821 gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca
     2881 taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc tgcaaaatag
     2941 cgataaaaat gcgttgattc gcgatctttt gaaaattaac gtgctggatg aagtgtatta
     3001 atatgaatgg ctggcaagga tgccggtaga aggatttact tcggagaggg ttatttcaga
     3061 taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac
     3121 tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattaggg
     3181 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
     3241 gactgaacta ccgctccacc gaattctttt acaaccaccg gttttatgac cggcttac
//
LOCUS       DAZFAH010000307         3286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFAH010000307 DAZFAH010000000
VERSION     DAZFAH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.309"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..1174
                     /gene="yiaY"
                     /locus_tag="ACHJWB_004693"
     CDS             23..1174
                     /gene="yiaY"
                     /locus_tag="ACHJWB_004693"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4316167.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1339..2877
                     /gene="aldB"
                     /locus_tag="ACHJWB_004694"
     CDS             1339..2877
                     /gene="aldB"
                     /locus_tag="ACHJWB_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4316168.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3096..>3286)
                     /locus_tag="ACHJWB_004695"
     CDS             complement(3096..>3286)
                     /locus_tag="ACHJWB_004695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316169.1"
                     /translation="VEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLG
                     TPMVIYGLVCPLTIETRD"
BASE COUNT          797 a          811 c          892 g          786 t
ORIGIN      
        1 tttacacttt caggagtgtg ttatggcatc ttcaactttc tttattcctt ctgtgaatgt
       61 catcggcgct gattcattga ctgatgcaat gaatatgatg gcagattatg gatttacccg
      121 taccttaatt gtcactgaca gtatgttaac gaaattaggt atggcgggcg atgtgcaaaa
      181 agcactggaa gaacgcaata tttttagcgt tatttatgat ggcacccaac ctaaccccac
      241 cacggaaaac gtcgccgcag gtttgaaatt acttaaagag aataattgcg atagcgtgat
      301 ctccttaggc ggtggttctc cacacgactg cgcaaaaggt attgcgctgg tggcagccaa
      361 tggcggcgat attcgcgatt acgaaggcgt tgaccgctct gcaaaaccgc agctgccgat
      421 gatcgccatc aataccacgg cgggtacggc ctctgaaatg acccgtttct gcatcatcac
      481 tgacgaagcg cgtcatatca aaatggcgat tgttgataaa catgtcactc cgctgctttc
      541 tgtcaatgac tcctctctga tgattggtat gccgaagtca ctgaccgccg caacgggtat
      601 ggatgcctta acgcacgcta tcgaagcgta tgtttctatt gccgccacgc cgatcactga
      661 cgcttgtgca ctgaaagccg tgaccatgat tgccgaaaac ctgccgttag ccgttgaaga
      721 tggcagtaat gcgaaagcgc gtgaagcaat ggcttatgcc cagttcctcg ccggtatggc
      781 gttcaataat gcttctctgg gttatgttca tgcgatggcg caccagctgg gcggtttcta
      841 caacctgcca cacggtgtat gtaacgccgt tttgctgccg catgttcagg tattcaacag
      901 caaagtcgcc gccgcacgtc tgcgtgactg tgccgctgca atgggcgtga acgtgacagg
      961 taaaaacgat gcggaaggtg ctgaagcctg cattaacgcc atccgtgaac tggcgaagaa
     1021 agtggatatc ccggcaggcc tacgcgacct gaacgtgaaa gaagaagatt tcgcggttct
     1081 ggcgactaat gccctgaaag atgcctgtgg ttttactaac ccgatccagg caactcacga
     1141 agaaattgtg gcgatttatc gcgcagcgat gtaatcatca tttccacaac ggctggcaaa
     1201 ttgtcagccg ctttttcaac tatctctgta acccttgccc gtaaattcgt gatagctgtc
     1261 gtaaagctgt taccgactgg cgaagatttc gccagtcacg tctacccttg ttatacctca
     1321 caccgcaagg agacgatcat gaccaataat cccccttcag cgcagattaa gcccggcgag
     1381 tatggtttcc ccctcaagtt aaaagcccgc tatgacaact ttattggcgg cgaatgggta
     1441 gcccctgccg acggcgagta ttaccagaat ctgacgccgg tgaccgggca gctgctgtgc
     1501 gaagtggcgt cttcgggcaa acgagacatc gatctggcgc tggatgctgc gcacaaagtg
     1561 aaagataaat gggcgcacac ctcggtgcag gatcgcgcgg cgattctgtt taagattgcc
     1621 gatcgaatgg aacaaaacct cgagctgtta gcgacagctg aaacctggga taacggcaaa
     1681 cccattcgcg aaaccagtgc tgcggatgta ccgctggcga ttgaccattt ccgctatttc
     1741 gcctcgtgta ttcgggcgca ggaaggtggg atcagtgaag ttgatagcga aaccgtggcc
     1801 tatcatttcc atgaaccgtt aggcgtggtg gggcagatta tcccgtggaa cttcccgctg
     1861 ctgatggcga gctggaaaat ggctcccgcg ctggcggcgg gcaactgtgt ggtgctgaaa
     1921 cccgcacgtc ttaccccgct ttctgtactg ctgctaatgg aaattgtcgg tgatttactg
     1981 ccgccgggcg tggtgaacgt ggtcaatggc gcaggtgggg aaattggcga atatctggcg
     2041 acctcgaaac gcatcgccaa agtggcgttt accggctcaa cggaagtggg ccaacaaatt
     2101 atgcaatacg caacgcaaaa cattattccg gtgacgctgg agttgggcgg taagtcgcca
     2161 aatatcttct ttgctgatgt gatggatgaa gaagatgcct ttttcgataa agcgctggaa
     2221 ggctttgcac tgtttgcctt taaccagggc gaagtttgca cctgtccgag tcgtgcttta
     2281 gtgcaggaat ctatctacga acgctttatg gaacgcgcca tccgccgtgt cgaaagcatt
     2341 cgcagcggta acccgctcga cagcgtgacg caaatgggcg cgcaggtttc tcacgggcaa
     2401 ctggaaacca tcctcaacta cattgatatc ggtaaaaaag agggcgctga cgtgctcaca
     2461 ggcgggcggc gcaagctgct ggaaggtgaa ctgaaagacg gctactacct cgaaccgacg
     2521 attctgtttg gtcagaacaa tatgcgggtg ttccaggagg agatttttgg cccggtgctg
     2581 gcggtgacca ccttcaaaac gatggaagaa gcgctggagc tggcgaacga tacgcaatat
     2641 ggcctgggcg cgggcgtctg gagccgcaac ggtaatctgg cctataagat ggggcgcggc
     2701 atacaggctg ggcgcgtgtg gaccaactgc tatcacgctt acccggcaca tgctgcgttt
     2761 ggtggctaca aacaatcagg tatcggtcgc gaaacccaca agatgatgct ggagcattac
     2821 cagcaaacca agtgcctgct ggtgagctac tcggataaac cgttggggct gttctgatat
     2881 aagaagctgg tcgcattggg tattcattgc ctgatgcgac gcttacgcgt cttatcatgc
     2941 ctacgggaac ctgaccgtag gccggataag gcgtacacgc cgcatccggc ataaacaaag
     3001 cgcacgttgt tacaatttac gttttaaacc tacgtgcgca ataacccaga ctacgccaac
     3061 agaagtgcgt ttctggggcg tgttttgcca ggagtttaat ctcgcgtttc gatggtgagc
     3121 gggcaaacca ggccgtaaat caccattggc gttccgaggc caatcagcaa taaacggtag
     3181 ataattgaag gtttaatacg ctttatctgt actgtggatg ttttactgga gttattcatt
     3241 agcttggtca atagttccat tatttcaata tgataaacct cgacct
//
LOCUS       DAZFAH010000308         3278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFAH010000308 DAZFAH010000000
VERSION     DAZFAH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.310"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(601..798)
                     /locus_tag="ACHJWB_004696"
     CDS             complement(601..798)
                     /locus_tag="ACHJWB_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316170.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(864..1134)
                     /locus_tag="ACHJWB_004697"
                     /pseudo
     CDS             complement(864..1134)
                     /locus_tag="ACHJWB_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1439..1627
                     /locus_tag="ACHJWB_004698"
     CDS             1439..1627
                     /locus_tag="ACHJWB_004698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316171.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1955..2149
                     /locus_tag="ACHJWB_004699"
     CDS             1955..2149
                     /locus_tag="ACHJWB_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316172.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2492..2620
                     /locus_tag="ACHJWB_004700"
     CDS             2492..2620
                     /locus_tag="ACHJWB_004700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316173.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2930..3265)
                     /locus_tag="ACHJWB_004701"
     CDS             complement(2930..3265)
                     /locus_tag="ACHJWB_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316174.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          967 a          754 c          721 g          836 t
ORIGIN      
        1 cagtttgtag gagctttctt ttttgaacaa tgatttgcag gacagttatg ctaaactagc
       61 ggaatcagta tcacaacaat aaatgactga tcatacctga aatttgaatt aacaggcatc
      121 atgaatgtca atagtctaag cggtgactac ttatatcgct tgcataaata ctaaacactg
      181 actagaaaag tattggataa ccccgtcact aaaagtaacg cttcggttat ttgctcggaa
      241 tgtattaacg ccaagattgt tagccgttac gccttatatc actggcgctg acaaatgttt
      301 cttttctaat ttactgaacc ggcgcagaca cgcaggaaag cacgccggca ccggtgtaat
      361 gccagttcta atcaataaga atggaatgac gaatcagtca ggaagggata acaacccgct
      421 gctgaacgga aggtatcagc aggaagatga acgggaatat cgccagcacc aagggcaggg
      481 ccgggttcca caataccagc gctggcattt tgcacattgc cagcgtaccg gcattacaac
      541 acgtttcatg ctgctgcccc ccaggattcc tcgattcgtt gctacatcca ctgctctact
      601 tcacttccac tgctccactt cacttttgta ccagcggctg ctgcgcccca gtttgatggg
      661 tttagggaaa tggccttcac tgatcaactt gtaaaaccat ttgtcggtca taccggtgaa
      721 actggtgata tagttcatgt cgatcaggga attatcggca cgcagaaata cgctgtcgtg
      781 gcttgttgta gtcaacatgg tcagaatgtc ctgtgagatt tatgaagatt gtgcgaatgc
      841 gggaaatcta ctgagctgtg ctttcagaac tggcctgtta cgggatacca gggattaccg
      901 gcggggtaac gggcttccgg atcatacaca ccacgattat cgcggacaaa atcactgaac
      961 gcccatatca cctctttaag tatgtcttcg cagtccggta aatgacgatc cagcgccaca
     1021 tcccgggtgg tacagctttg atgcgcccgg tgacacaaag cccggattgt tccagacatc
     1081 ctgaatcaaa cgccccagat taggggcgtc gaaatatgcc tctctgacca ttatattccg
     1141 gcgtataggt agcaggtctg aagtgacaat gcgtcacctg acgttaaaag tcacccaaga
     1201 tgaccccagt gggggcaaca agtataaaac cgttttttga ttaagtggtc ggatatgaaa
     1261 aggggaaggt ttatgtgaaa aatacctgtg ttttctttga aaacacacaa cagtatcagg
     1321 ttatttatgt ttatacagtg cgttccaaac tgacctgaat aattacgcaa gttgcaacaa
     1381 taaaacattt ttgccgtagt gtgaggtact ccggtaaatt gcttcccagg tgcagacaat
     1441 gactaacgaa gaactacagg caatcgcatt atccatgctt gagcgccagc gcgaaaaagt
     1501 acaccagcaa cgagagtctt tgcttaaccc atctccattc accaggccaa aacgattgga
     1561 gcttgaagca ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc
     1621 aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac catcagcgcg
     1681 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
     1741 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
     1801 gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat ctcctttagt
     1861 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
     1921 acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
     1981 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
     2041 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
     2101 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
     2161 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatatcc
     2221 actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta caagcacact
     2281 agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata cactcagtgg
     2341 cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc catctggtca
     2401 taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc ccaatatcct
     2461 gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag agttctcggt
     2521 acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg aaattatctc
     2581 ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta tacgatgcat
     2641 tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt ggcaatatcg
     2701 aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccgcacaaa
     2761 ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct aaaacgcata
     2821 gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct gcattatgtt
     2881 aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac tatacggtat
     2941 cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc acaatactga
     3001 atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct gcgttccggg
     3061 cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc cttttaccag
     3121 tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt atagcattgt
     3181 ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca gtccgttttt
     3241 cataacccac tgtactgata accataatct aatcagta
//
LOCUS       DAZFAH010000309         3262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFAH010000309 DAZFAH010000000
VERSION     DAZFAH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.311"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..1160
                     /gene="rfaD"
                     /locus_tag="ACHJWB_004702"
     CDS             228..1160
                     /gene="rfaD"
                     /locus_tag="ACHJWB_004702"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4316175.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1170..2216
                     /gene="rfaF"
                     /locus_tag="ACHJWB_004703"
     CDS             1170..2216
                     /gene="rfaF"
                     /locus_tag="ACHJWB_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4316176.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQMVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2220..3212
                     /gene="rfaC"
                     /locus_tag="ACHJWB_004704"
     CDS             2220..3212
                     /gene="rfaC"
                     /locus_tag="ACHJWB_004704"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4316177.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
BASE COUNT          828 a          798 c          859 g          777 t
ORIGIN      
        1 caaatgcttc cgttatttat catacatcgg aatattgata ctaaagcact attcacatgc
       61 aaaaccaaca tccgccatga aggactagct aaaacccaaa ctagtttgtt gcaattagca
      121 tccttgcacc tctatgtaaa gggctgaagg gattcggatg tgatggtatg attacagaca
      181 ttcgtgtctg agattgtctc tgactccata attcgaaggt tacagttatg atcatcgtta
      241 ccggcggcgc gggctttatc ggcagcaaca tcgttaaagc cctgaatgat aaaggcatca
      301 ccgatattct ggtggtggac aacctgaaag acggcaccaa gtttgtgaac ctggtggatc
      361 tgaatatcgc agactatatg gataaggaag acttcctgat ccagattatg gctggcgaag
      421 agttcggcga tgtcgaagcg attttccacg aaggcgcgtg ctcttccacc accgagtggg
      481 acggcaagta tatgatggat aacaactatc aatactccaa ggagctgctg cactactgcc
      541 tggagcgtga aatcccgttc ctgtacgctt cttccgcagc cacctacggc ggacgcacct
      601 ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa cgtctacggt tactcaaaat
      661 tcctgtttga tgaatatgtt cgccaaatcc tgccggaagc gaactcgcag attgttggct
      721 tccgctattt caacgtttat ggaccgcgtg aaggccataa aggcagcatg gcgagcgtcg
      781 ctttccatct caacacccag cttaataacg gtgaatcacc gaagctgttc gaaggtagcg
      841 agaactttaa acgcgacttc gtctatgttg gcgatgtggc agatgtaaac ctgtggttcc
      901 tggaaaatgg cgtttccggc atcttcaacc tcggcaccgg tcgtgcggaa tccttccagg
      961 ctgtagctga tgctacgctg gcttatcaca agaaaggcca gatcgaatac attccgttcc
     1021 cggataagct gaaaggccgc taccaggcgt tcactcaggc agatctgaca aatctgcgcg
     1081 cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg tgtaacggaa tacatggcct
     1141 ggctgaatcg cgacgcataa gagctctgca tgaaaatact ggtgatcggc ccgtcttggg
     1201 ttggcgacat gatgatgtcg caaagtctct atcgcacgct ccaggcgcgc tatccccagg
     1261 cgataatcga tgtgatggca ccggcatggt gccgtccatt attatcgcgg atgccggaag
     1321 ttaacgaagc tatccctatg cctctcggtc acggagcgct ggaaatcggc gaacgccgca
     1381 aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc ctacgtctta cctaactcct
     1441 tcaaatccgc attagtgcct ttcttcgcgg gtattcctca tcgcaccggc tggcgcggcg
     1501 agatgcgcta cggtttactc aacgatgtac gcgtgctcga taaagaagcc tggccgctaa
     1561 tggtggaacg ctatatagcg ctggcctatg acaaaggcat tatgcgcaca gcacaagatc
     1621 tgccgcagcc attgttatgg ccgcagttgc aggtgagcga aggtgaaaaa tcatatacct
     1681 gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg tttttgcccg ggtgcggagt
     1741 ttggtccggc aaaacgctgg ccacactacc actatgcgga gctggcaaag cagctgattg
     1801 atgaaggtta tcagatggtt ctgtttggct cggcgaaaga tcatgaagca ggcaatgaga
     1861 ttcttgccgc tttaaatacc gagcagcagg catggtgtcg gaacctggcg ggggaaacac
     1921 agcttgatca agcggttatc ctgattgcag cctgtaaagc cattgtcact aacgattctg
     1981 gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt tgccctgtat ggtccaagta
     2041 gcccggactt cacaccgccg ctatcccata aagcgcgcgt gatccgtctg attaccggct
     2101 atcacaaagt gcgtaaaggt gacgctgcgg agggttatca ccagagcttg atcgacatta
     2161 ctccccagcg cgtactggaa gaactcaacg cgctattgtt acaagaggaa gcctgacgga
     2221 tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt tctccatacg ttgcccgcac
     2281 tcactgatgc ccagcaggca atcccaggga ttaagtttga ctgggtggtg gaagaagggt
     2341 tcgcacagat tccttcctgg cacgctgccg ttgagcgagt tattcctgtg gcaatacgtc
     2401 gctggcgtaa agcctggttc tcggccccca taaaagctga acgcaaagcg tttcgtgaag
     2461 cgctacaagc agagaactat gacgcagtta tcgacgctca ggggctggta aaaagcgcgg
     2521 cactggtgac acgtctggcg catggcgtaa agcatggatt ggactggcaa accgctcgcg
     2581 aacctttagc cagcctgttt tacaattgta agcatcatat tgcaaaacag cagcacgccg
     2641 tagaacgcac ccgcgaactg tttgccaaaa gtttgggcta tagcaaaccg caaacccagg
     2701 gcgattatgc tatcgcacag cattttctga cgaacctgcc tacagatgct ggcgaatatg
     2761 ccgtatttct tcatgcgacg acccgtgatg ataaacactg gccggaagaa cactggcgag
     2821 aattgattgg tttactggct gattccggaa tacggattaa acttccgtgg ggcgcgccgc
     2881 atgaggaaga acgggcgaaa cgactggcgg aaggatttgc ttatgttgaa gtattgccga
     2941 agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc taaatttgta gtgtcggtgg
     3001 atacggggtt aagccattta acggcggcac tggatagacc caatatcacg gtttatggac
     3061 caaccgatcc gggattaatt ggtgggtatg ggaagaatca agtagagtgt cgttctacaa
     3121 gtatgtctct tgcagatttg ccagctcaaa ccgtttttca aaatttaaat cttgaaataa
     3181 taaccaataa gttgacatcg gagataagat gacctcaaca ttatttttct ctctcgagaa
     3241 aaaaaactgg atagcgtact gg
//
LOCUS       DAZFAH010000310         3253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFAH010000310 DAZFAH010000000
VERSION     DAZFAH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.312"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..200)
                     /locus_tag="ACHJWB_004705"
     CDS             complement(<1..200)
                     /locus_tag="ACHJWB_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4316178.1"
                     /translation="MSEKDESNFIERFTVRMPDGMRSAIAERAKRNGRSMNSEIVQIL
                     EDALSSDVSSVDKSLDLKQIKS"
     gene            324..497
                     /locus_tag="ACHJWB_004706"
     CDS             324..497
                     /locus_tag="ACHJWB_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4316179.1"
                     /translation="MSDVLYSGRKSQSFQLRLPARMKEEIRRVAEMDGISINSAIVQR
                     LAKSLREERANAQ"
     gene            571..1209
                     /locus_tag="ACHJWB_004707"
     CDS             571..1209
                     /locus_tag="ACHJWB_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO4316180.1"
                     /translation="MNIVAKSDYNFQGFTFNPVTEGGSIWFTSTELAKALGYKKTDAI
                     SQIYARNADEFSDSMSLTLNMKVNGINNSLRNKSVRVYSLRGAHLVAMFASTPKAKEF
                     RRWVLDILDRQAECSPIAKQFTDEELVNLCYLQLWMEKSQQMCKHIYPGMKQIGSELS
                     GKIYDIAYETRYMSEETKKSLLREMKNLDPNNFVVKNAQPMLAKLRGEEWIH"
     gene            1297..2025
                     /locus_tag="ACHJWB_004708"
     CDS             1297..2025
                     /locus_tag="ACHJWB_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316181.1"
                     /translation="MTFKNVANIGSVVTDKTIDSQYLLEMVNHARRQCGEKEVRNNDF
                     IARIKDELEGEHYEIFVVQKSNKTTSEKVVMSIKQALRVAARESKAVRRSLVDQLESM
                     QEAHIKSGKSSSGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPIVG
                     FNAIPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQ
                     VEVFRYNAEGVKALQHLIHGSNVA"
     gene            2025..2246
                     /locus_tag="ACHJWB_004709"
     CDS             2025..2246
                     /locus_tag="ACHJWB_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316182.1"
                     /translation="MAKKKYGIMPPRIKGRARVKGDAGRYHILGVLWHERALILSRPH
                     GYIEKVSIDRVEILPLTPEEEETYGLFDN"
     gene            complement(2261..2548)
                     /locus_tag="ACHJWB_004710"
     CDS             complement(2261..2548)
                     /locus_tag="ACHJWB_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4316183.1"
                     /translation="MPFHPFGEALMMYSKYDEAQFHLRLTHELHAKIKQRAKMNNRSI
                     NSEIVATMEESLSKPSPVSGYRDEEERLASLISERVKEVAADILRKEKTRD"
     gene            2651..>3253
                     /locus_tag="ACHJWB_004711"
     CDS             2651..>3253
                     /locus_tag="ACHJWB_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFO4316184.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIPVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAKQFY
                     FPNVLKYDPDQAYYRAEKDIKRSGAIETKIQDAINSSVSGQTVLTINEDEVVDSINND
                     YGVEIDARRGRVFNRDGVQLSTYTNPRNRFVIGQEYLNYVT"
BASE COUNT          995 a          667 c          722 g          869 t
ORIGIN      
        1 aaagatttta tttgtttaag atctaatgat ttgtctactg atgagacgtc agatgataac
       61 gcatcctcaa gaatttgaac tatctctgag ttcatagatc gaccgtttcg tttagccctt
      121 tccgcaatag cactacgcat gccgtcaggc attctaaccg taaatctttc tatgaaattt
      181 gattcgtctt tttctgacat tttcttattc gcaaggcata tgagcaataa aaaacacagt
      241 agcatcatat tgacattatg agcaatggca tcataatggt gtcatgacat catggtgatg
      301 tcattatgac ggagatattg atgatgagcg atgttcttta cagtggtcgt aaaagtcaga
      361 gtttccagct tcgtttgcca gcgcgaatga aggaggagat cagacgcgta gctgaaatgg
      421 atggaatttc tatcaactct gcgattgtgc agcgactggc taaaagcctg agagaggaaa
      481 gagctaatgc ccagtaaaaa tagtgaagcc cggaagtgcg cgaacacaaa ccgggcctct
      541 atgtcagtaa ccgtactcaa ggaaactaac atgaatattg tagcaaaatc agattataac
      601 ttccaaggat tcacttttaa ccctgtaaca gagggcgggt ctatctggtt tacctccacc
      661 gaactagcta aggctctcgg ctataaaaaa actgatgcca tcagccaaat ttatgcccgt
      721 aatgctgacg aattttctga ctcaatgtca ttgaccctca atatgaaggt caacggaata
      781 aacaatagct tacgtaacaa atcggtcaga gtttactcac tccgaggcgc tcacttggtg
      841 gcgatgtttg cttctacgcc caaggccaaa gagttccgcc gctgggtgct ggatattttg
      901 gatcggcagg cagaatgctc accgattgca aaacagttta ctgacgaaga actggttaat
      961 ctctgctact tacaattgtg gatggagaag agtcaacaaa tgtgcaaaca catctaccct
     1021 ggaatgaagc aaattggttc tgagctttca ggaaagattt acgatattgc atatgagact
     1081 cgctacatgt cagaagaaac caagaaatcg cttcttcgtg aaatgaagaa tcttgatccc
     1141 aacaattttg tcgtaaagaa cgctcagcca atgctggcaa aacttcgcgg cgaggaatgg
     1201 attcattgat tggcgcacag gacggcgcaa aagaaaaccg ccagtgtgct gctggcggcc
     1261 tatgtcacac ccttactacc acataaggaa tgcctaatga cttttaagaa tgtagcaaac
     1321 atcggatccg ttgtcacgga taaaaccatt gatagccagt acctgttaga gatggtcaat
     1381 catgctcgta gacagtgcgg ggaaaaagaa gtccgaaata acgactttat tgcacgcatt
     1441 aaggatgaac ttgaaggtga gcactacgaa attttcgtag ttcaaaaatc aaacaagaca
     1501 acttctgaaa aagttgttat gtcaattaag caagcccttc gagtggctgc tcgtgaatct
     1561 aaagctgttc gccgctcact tgtagaccaa cttgaaagta tgcaagaagc gcacattaaa
     1621 agcggtaaat cgtcgagtgg acttgttgag tatcgccagg cgcgaacatt gaaaatgacg
     1681 gttgaagctg ttaccaatct gttcgatttg atgccaaatc ttgcgccgga agcaaagcag
     1741 actgcggcag caagcataat caacccgatc gttggtttta atgcaatacc tcttccagca
     1801 atagaagagc attactactc agcaggggag gttgcagagc agcttggagt aacggccaac
     1861 aagattggtc gcattgctaa cgcaaacaac ctcaaaactg agcagtacgg taagttcttc
     1921 ctggataaat ctgcgcattc cagcaaacag gtggaagtat tccgctacaa tgcggaaggt
     1981 gttaaagcac tacaacacct gattcatggg agtaatgttg cataatggca aagaaaaaat
     2041 atggcattat gccgccaaga atcaaaggaa gagccagggt aaaaggcgat gctgggaggt
     2101 atcacattct tggagttctg tggcatgaga gagctttaat tttaagtaga cctcatgggt
     2161 acattgaaaa ggtatctata gatagagtag agattcttcc ccttacacct gaagaagaag
     2221 aaacgtacgg actttttgat aactaaccaa ctaaacccgc ttaatcgcgg gttttttctt
     2281 ttctaaggat atcagccgca acttctttta ctcgttccga gattaatgag gccagcctct
     2341 cttcttcatc acgatacccg cttacaggtg atggtttgga gagtgattct tccatcgtag
     2401 ccacaatttc ggaattgata gacctgttat tcatttttgc acgctgttta atcttggcgt
     2461 gcaactcgtg cgtaagcctc aagtggaact gcgcctcatc gtatttgctg tacatcatca
     2521 atgcctcacc aaatgggtgg aatggcatcg taaaacctac tgtacaaatc aacaatcgta
     2581 ccgtttcggt atgcaacaaa taccaaccgt agccacgctg cggcgattca ttgtatctgg
     2641 agcaaattaa atgacagaca ttacagccaa tgtgatcgta tcgatgcctt cgcaactttt
     2701 cactatggcg cgttctttta aagcggttgc caatggcaaa atttatatcg gtaaaattga
     2761 cacggacccg gtaaatcctg aaaaccagat tccggtttat gtagagaacg aagacggctc
     2821 tcacgttcct gtttcgcaac caatcatcat taacgctgct ggatatccgg tatataacgg
     2881 acagattgct aaattcgtaa ctgtgcaagg ccattctatg gctgtttatg atgcatatgg
     2941 tgcgaagcag ttctattttc ctaatgttct taagtatgac ccagatcagg catattatcg
     3001 tgctgaaaaa gacattaaaa gatctggcgc tatcgaaaca aaaatacaag acgctattaa
     3061 ttcatccgtc tcaggtcaga cggtgttaac aattaatgag gatgaggttg ttgattctat
     3121 taacaatgat tatggtgtgg aaatcgatgc tagaaggggg agagttttta atagagatgg
     3181 tgtccagtta tcaacataca caaatccgag gaatagattt gtcataggtc aagagtattt
     3241 aaattatgtt aca
//
LOCUS       DAZFAH010000311         3215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFAH010000311 DAZFAH010000000
VERSION     DAZFAH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.313"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHJWB_004712"
     CDS             <1..783
                     /locus_tag="ACHJWB_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4316185.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARPEQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            790..1536
                     /locus_tag="ACHJWB_004713"
     CDS             790..1536
                     /locus_tag="ACHJWB_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4316186.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLVEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            1558..2274
                     /locus_tag="ACHJWB_004714"
     CDS             1558..2274
                     /locus_tag="ACHJWB_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4316187.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2307..2588
                     /locus_tag="ACHJWB_004715"
     CDS             2307..2588
                     /locus_tag="ACHJWB_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4316188.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2585..2812
                     /locus_tag="ACHJWB_004716"
     CDS             2585..2812
                     /locus_tag="ACHJWB_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316189.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2805..3116
                     /locus_tag="ACHJWB_004717"
     CDS             2805..3116
                     /locus_tag="ACHJWB_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316190.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          879 a          694 c          981 g          661 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc ctgcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcgc gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggccgga gcaatcacac
      361 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg gtcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatactgg atggtgtgag gcgttacgca
      601 agatacctgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
      781 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
      841 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgtt
      901 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
      961 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     1021 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     1081 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     1141 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     1201 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     1261 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     1321 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     1381 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gcacgcttct tggtgagagg
     1441 attatggacc gcatgaccat gaacggtggt cggtgggtga cgtttaactg ggatagctgg
     1501 cgtccaaatg tcagcaatca gagggttgtg aagtaatttt tgttggagga cgttttaatg
     1561 gaaactgtat ttgacgcact gaaagcactg aaaaaagcct cttcacaggt agtggcatcg
     1621 cgccttggaa tcagccgcga agatgctgtc aacgaactgt ggaaactgaa gcgccgtggt
     1681 gaagcggata acaagggttc gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg
     1741 tcaccggtac cgaaagtgac agcgcaaatg ctgactgagg cgattgaaca tcatggccca
     1801 caaacggcgg atgagctggc actgatgttc gggattacct cccgccgggc gaattcatca
     1861 ctggccatgg caatcagcaa agggcgtctg attcgcgtga atcagggcgg taaatttcgg
     1921 tactgcatac cgggcgctga tttaccggca gagccgaaag ccgcatccat agcggaaacg
     1981 gatggtaaag cctttcctca gccagcaggt gttgcgttac cagtcgggga agcggaaaca
     2041 caggaagaaa taaaaacgga aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc
     2101 agaaagcatc cggatggtct gattttacca tcgctgcatg tggctaaccg cgagctgcgc
     2161 cgggcaaaag gtcaggttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
     2221 aagtgccggg atattctccg ggatattacc gccaccagag aacagcagcg gtgagtgggt
     2281 ggaagacgtg gtgccgggcg gaaatcatga tactccggca gtgtgcggga acgaggaagg
     2341 taaaaagcgt tggcgcactt atcggacgaa ctgaagcggc agtgagaacg aaggcacggg
     2401 agctgggcat cagcatgatg ttacgtggtg attttcaccc gtcggcaaaa tattctcagc
     2461 gtgatattga gctggcgcgg caactgcatc agagaggcat gcaaagaagg gaaattgcca
     2521 gaaaattagg catgccgctg cgcatagtga ataactacgt ttatttcgac aggagggtgt
     2581 ctgcgtgaaa atcctgtatc aggattacgg cccggtgggg caggtggtta tcagcagtac
     2641 tgtaatggag tttcggaagc ataaccgtgt ggtggatgct gtgctgttaa cctgtccggg
     2701 gatatcggcg agtcgtgcag gtgtgtttat tatgaagacg aaattatatg gcagtaaggc
     2761 gtggataaag aaggcgtatc gtgtagcgtt gcaggaggtt aacagtgagt aaaattaaag
     2821 aaatgccggt agttcgtgac ggatatggct actggacaca tcctgaatat gaaaaattct
     2881 gtgatggtcg ggaatatatt tcaacggaag agtttaacgc ctggatggag gaaaataatc
     2941 ttcaatacgt cctctgcttc agagatgaag gatgtgctga ccttgatgcg tgtgatgctg
     3001 atatttctgc atgggaaccg gaacgaccag agggcgatgg ctggtttatt ggttccattc
     3061 atgatacgga agatggcccg gtttgtgtct ggttgcgaaa taaggctgaa gcataaaggc
     3121 gataaaccaa ctaacaacta aatactgaag atttaaatca gaaacgattt ttattaaatc
     3181 cttaaccgga gggattcctg caccctcaga acatc
//
LOCUS       DAZFAH010000312         3189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFAH010000312 DAZFAH010000000
VERSION     DAZFAH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.314"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..640
                     /locus_tag="ACHJWB_004718"
     CDS             <1..640
                     /locus_tag="ACHJWB_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316191.1"
                     /translation="ESAMDSFSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVL
                     SMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGD
                     KYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYADGGYVGGAGSPAQMRRAEGINF
                     NQNNHVVIQNDGPNGQAGPQMLKAVYDMARKGAQDELRLQLRDGGLLSGSGR"
     gene            637..966
                     /locus_tag="ACHJWB_004719"
     CDS             637..966
                     /locus_tag="ACHJWB_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316192.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVELSA
                     EFKQVVN"
     gene            966..1664
                     /locus_tag="ACHJWB_004720"
     CDS             966..1664
                     /locus_tag="ACHJWB_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4316193.1"
                     /translation="MQDIHEETLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            1675..2418
                     /locus_tag="ACHJWB_004721"
     CDS             1675..2418
                     /locus_tag="ACHJWB_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4316194.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            2415..2993
                     /locus_tag="ACHJWB_004722"
     CDS             2415..2993
                     /locus_tag="ACHJWB_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316195.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          663 a          774 c         1080 g          672 t
ORIGIN      
        1 ggaaagtgcc atggacagtt tttcgcaggt taaaagcgcg gccacgcaga cctttgacgg
       61 tattgcacag aatatggcgg cgatgctgac cggcagtgaa cagaactggc gcagcttcac
      121 ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg tggggattgt
      181 cgggagtatc ggtagtgcca ttggcggggc tgttggtggc ggcgcatccg cgtcaggcgg
      241 tacagccatt caggccgctg cggcgaaatt ccattttgca accggaggat ttacgggaac
      301 cggcgacaaa tatgagccag cggggattgt tcaccgtggt gaatttgtct tcacgaagga
      361 ggcaaccagc cggattggtg tcggcaacct gtaccgtctg atgcgggggt atgcggatgg
      421 cggttatgtc ggcggtgccg gaagtccggc gcagatgcgg cgggcggaag gcattaattt
      481 taatcagaac aaccatgtgg tgattcagaa cgacggcccc aacgggcagg ccggaccaca
      541 gatgctgaag gctgtgtatg acatggcccg taaaggtgca caggatgagc tccggctgca
      601 gttacgtgat ggcggtctgt tatcagggag cgggcgatga aaacatttcg ctggaaagtg
      661 aagccggata tggaggtgaa ctcgcagcca tcggtgcgtg aagtgcgttt tggtgacggg
      721 tattcgcagc gtatggctgc ggggctgaat gctgacctga agacgtacag ggtgatgctt
      781 tccgtgaccc gggaggaggc ccggcatctg gaagcgttcc tggcagagca cggaggctgg
      841 aaggcatttt tgtggaagcc accctatgca taccggcaga taaaggtgac ctgtgccggg
      901 tggtctgcgc gggtcgggat gttgcgcgtt gagctcagcg cggagtttaa gcaggtggtg
      961 aactgatgca ggatattcac gaagaaactc ttaacgagtc ggttaagtca gagcagtcac
     1021 cgcgggtggt gctctgggaa atcgacctga cggtgcaggg cggtgagcgg tattttttct
     1081 gcaatgagct gaatgaaaaa ggggagccgg tgacctggca ggggcgtgaa tatcaggcgt
     1141 acccgattga gggcagcggc tttgagatga acgggaaggg cagcagtgcc cgcccgtcgc
     1201 tgacggtgtc caatcttttc ggccttgtca cggggatggc ggaggatttg cagagcctgg
     1261 tgggtgccac ggtggtccgc cgccgggtgt atgcacgttt tctggatgcg gtgaatttcg
     1321 ttgcgggcaa tccggaagcg gacccggagc aggagctgag cgaccgctgg gtggtggagc
     1381 agatgtcgca gctgacagcc atgacggcct cgtttgtgct ggccacaccg accgagacgg
     1441 acggagcgct gtttcccggt cgtatcatgc tggcgaacac ctgtatgtgg acctaccgct
     1501 ctgatgagtg tggttacacg ggcggggctg tggcggatga gttcgacaac cccaccacgg
     1561 atatccgtaa ggacagatgc agcaagtgca tgcgcgggtg tgagatgcgc ggcatggtgg
     1621 ctaattttgg cggtttcctt tccattaata aactttcgca gtaaatcccg gtttatgaca
     1681 cagactgaat cagcgattct ggcgcatgcc cggcggtgtg cgcctgcgga gtcgtgcggc
     1741 ttcgtgatag gcaccccgga gggcgaacgg taccagccct gcgtgaatat ctccgcagag
     1801 ccggaggcgt attttcgtat tgcgccggaa gactggctgc aggcagagat gcagggggag
     1861 attgtggcgc tggttcacag ccaccctggt ggtctgccct ggctgagcga ggccgaccgg
     1921 cggctgcaga taaagagtgc cctgccctgg tggctggtct gccgggggga aattcacaaa
     1981 ttccgctgtg tgccgcacct gaccgggcgt cgttttgagc acggggtgac ggactgttac
     2041 accctgttcc gggatgcata ccatctggcg gggataacgc tgccggattt tgtgcgtgag
     2101 gatgactggt ggcgcaacgg tcagaacctt tacctggaca atatggcggc gactggtttt
     2161 taccgggtgc ccctgtcctc tgcacaggcg ggcgatatcc tgctgtgctg ctttggcgca
     2221 tcggtggcca atcatgccgc catatactgc ggcaacggtg aactgcttca ccatctgcct
     2281 gaacaactga gtaaacggga gaggtattct gaaaaatggc aacgacgaac gcattctgtc
     2341 tggcgtcacc gccactggca cgcatctgcc ttcacgggga tttgcaacga tttggccgcc
     2401 gcctcagcct gtacgtgaac acggcagcgg aagccatccg tgccctgtcg ctgcagatgc
     2461 cgggattccg ccgtcagatg aacgaaggct ggtaccagat acgtattcgc ggtgaggaca
     2521 cggcaccgga ggcggtgtac gcccgtcttc acgaacctct gggtgagggg gcggtcatcc
     2581 atattgtgcc gcgactggcc ggagccggaa agggcggact gcagattgtg ctgggggcag
     2641 cagccatcgt gggctctttc ttcaccgccg gcgcaacgat ggcgttgtgg ggcgcagccc
     2701 tgagtgccgg agggctgact gccaccacga tgctgttctc actgggtgcc agcatgatac
     2761 tgggcggtgt ggcccagatg ctggcaccga aggcaaaaac accggattac cgcgcaacgg
     2821 ataacggtaa acagaacacg tatttttcgt cactggataa catgattgct caggggaacc
     2881 cgatgccggt gccttatggt gaaatgctgg ttggttcacg acggatatcc caggacatca
     2941 gcacccgtga tgagggcgga gacgggaaag tggtggttat cgggcgggga tgaaaataaa
     3001 aaaatcccgc agagttagcg gagctgcggg agagaacgat gaagattaac gttatggagt
     3061 tatttttcag gcatcaaaaa agtaatgcag cgtcattatt gcggctacag gcaattgccg
     3121 gaaatgtgaa gagtttcaga aattttattc cgtcatgaca caggcaccct ccggggtgcc
     3181 tgttgtttt
//
LOCUS       DAZFAH010000313         3176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFAH010000313 DAZFAH010000000
VERSION     DAZFAH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.315"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..556
                     /gene="yfgH"
                     /locus_tag="ACHJWB_004723"
     CDS             38..556
                     /gene="yfgH"
                     /locus_tag="ACHJWB_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4316196.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            572..1111
                     /gene="yfgI"
                     /locus_tag="ACHJWB_004724"
     CDS             572..1111
                     /gene="yfgI"
                     /locus_tag="ACHJWB_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4316197.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1331..1813)
                     /locus_tag="ACHJWB_004725"
     CDS             complement(1331..1813)
                     /locus_tag="ACHJWB_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO4316198.1"
                     /translation="MIWVFVKAYWKQMLIVLMLAALFIVGVVAWNIHGSRQYDAGYAQ
                     AEEDRKDEEDKVRQYYEQEKVTNEREAQQRIDQARNDALDAAARAGRLQQQLFAIREQ
                     LRQYNAIVGAGTSAADTGVLLADVLSKSLERNRQLAEYADRAAEAGRVCEKQYDTLTR
                     "
     gene            complement(1810..2439)
                     /locus_tag="ACHJWB_004726"
     CDS             complement(1810..2439)
                     /locus_tag="ACHJWB_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HFO4316199.1"
                     /translation="MEIKQFQRAAGISEALAARWFSHITSAMKEFGISKPEDQAMFIA
                     QVGHESGGFTRLQENFNYSVTGLANFVQAGRLTQGQANALGRRAGEPPLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLLENPELLAQDEYAA
                     RSAAWFFSSKGCMKYTGDIARVTLIINGGRNGIDDRRARYVTASKVLAV"
     gene            complement(2429..2737)
                     /locus_tag="ACHJWB_004727"
     CDS             complement(2429..2737)
                     /locus_tag="ACHJWB_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4316200.1"
                     /translation="MVTHELFLLITNAVICTGIAIRVVTFQRNGSQHRRWGGWIAYFL
                     IVAAASIPVRVVYAIWLRTPMAVDLSEVIINAVMLAAVLKTRGNVVQIFKITRSKYGD
                     "
     gene            complement(2724..3128)
                     /locus_tag="ACHJWB_004728"
     CDS             complement(2724..3128)
                     /locus_tag="ACHJWB_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO4316201.1"
                     /translation="MSFQITNDHVNQYLTVGSLAAVIGGTPPEVALGSLAGAVIFVTS
                     AIEYPIRRRVLLSVLSFICGLLFYKPAASILIGIASLIPTITQDSFEKGIVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELMPGRKDDGNA"
BASE COUNT          919 a          788 c          738 g          731 t
ORIGIN      
        1 aatgtcttat ttttcgtaat gtgtataaca aggaatagtg atgaaattta aaaaatgtct
       61 tctgcctgtg gcaatgttag cgtcattcac tctggcagga tgccagtcaa atgctgacga
      121 tcatgccgcc gatgtttatc aaaccgatca actgaatacc aaacaagaaa ctaaaaccgt
      181 taatattatt tccattcttc ccgcaaaagt tgccgtagac aactcccaaa ataaacggaa
      241 cgcacaagcc ttcggcgcgc ttattggcgc agtcgctggc ggtgttatcg gccacaacgt
      301 cggttctggc agcaattccg gaacgacggc aggggcagtt ggcggcggag ctgtaggcgc
      361 ggcagcgggt tctatggtga atgataaaac cttagtggaa ggtgtttctt taacatataa
      421 ggaaggcacc aaagtgtata cctctaccca ggtgggtaaa gagtgccagt ttacgacagg
      481 tttagccgtt gttattacca caacgtataa cgaaacgcgt attcagccaa atactaaatg
      541 tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt gcgccatctt
      601 ggcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct ctgctgtagc
      661 agaagctgaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat gggtcgcgga
      721 gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aatgcccagg ccgccgctaa
      781 caaaagagcg gcaacggcag cagcaaataa gaaagctcgt caggataaac tggacgccga
      841 agcctctgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgca gcttagagat
      901 tcagaaacaa aaactggcgc tggcgaaaga agaagcccgc gttaagcgag aaaacgaatt
      961 tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat ctgaagctga
     1021 cgccaacaga aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg gcaaagcaga
     1081 agagaacaaa tcggacagct ggtttaatta atcgatgtta gtaacttcaa gcctatgatt
     1141 cttgaagata aaaaaccctc tgtagtaaca gagggttttg ttcattcata gtgcagggat
     1201 taaaatcatt cccactcaat tatttacgga aaccataacc aattgagtga taacactttt
     1261 ccaaatctca attttcctcg taccgtttta tataccgtca caggaaatca gtaccatgaa
     1321 aaatgccata ctatctggtc agggtgtcgt actgtttttc acagactctt ccggcttcgg
     1381 ctgcccggtc agcatactct gccagttgtc tgtttctctc gagagatttg ctgagcacgt
     1441 cggcaagcaa aaccccggtg tctgcggctg acgtcccagc gccgacaatg gcgttatact
     1501 gcctgagctg ctcacggatg gcaaagagtt gttgctgcaa ccggccagcg cgagcggcag
     1561 catcaagagc atcattgcgc gcctggtcga tcctctgctg agcttcacgt tcattggtca
     1621 ctttctcctg ttcgtagtac tgacgaactt tgtcttcttc gtctttgcgg tcttcttccg
     1681 cctgcgcata cccggcatcg tactggcggc tgccgtgtat attccaagca accactccga
     1741 cgatgaacag agcagcaagc atcaacacga taagcatctg tttccagtat gcttttacga
     1801 atacccagat catacagcca gcaccttact ggcagtgacg taccgcgcgc gccggtcgtc
     1861 gatgccattc ctgccaccat tgataatcag agttacacgt gcaatatcgc cggtatactt
     1921 catgcaacct ttgctggaga agaaccacgc cgcgctacga gccgcgtatt cgtcctgcgc
     1981 caacagttca ggattctcca gcaggtcaac tttcagaccg tttccacagt cacgatagtt
     2041 attcaaaccg gtaatctgga taagcccgcg cccacggtaa ttccagccat caccaggggc
     2101 attgttcccc atgcgtttgc tgtacaccag atttgcgatc gcgcgctggc gctcgagtgg
     2161 caatggtggt tcaccagcac ggcgaccaag tgcattagcc tgcccctgag tgagacgccc
     2221 agcctgaacg aagttagcca atccggttac gctgtagttg aaattttcct gcaaccgggt
     2281 gaagcctcca gactcatgcc cgacctgagc aataaacatt gcctgatctt ctggtttgct
     2341 gataccaaac tctttcatcg cagaagttat atgcgagaac cagcgagcgg ccagcgcctc
     2401 gctaatacca gcagctcgct ggaattgttt aatctccata tttagacctc gttattttga
     2461 aaatctgaac gacgttaccg cgagttttaa gaaccgcagc aagcatgaca gcgttgataa
     2521 tgacctcaga taaatccaca gccattggcg tgcgtaacca gattgcataa acgacacgaa
     2581 ctggaatact cgcagcagca acaatcagga aataagcaat ccacccaccc catcttcgat
     2641 gttgagatcc gttacgctgg aaagtgacaa cgcgaattgc tatgccagta caaataaccg
     2701 cattggtgat aagtaaaaaa agctcatgcg ttaccatcgt cttttctccc cggcattaat
     2761 tcgcgtggat tatcggaacg gtgatagagc cagataccaa tacgcacagc gacaattgct
     2821 gacacgaatg cgccagcaga gaaaacaatc cctttctcaa aagagtcctg tgtgatggta
     2881 gggatcaggc tggctatgcc aataagaatt gatgctgctg gtttgtaaaa gagaaggccg
     2941 caaataaagc tgagcactga cagaagaaca cgacgacgga ttggatactc gatagcagag
     3001 gtaacaaaaa tcacagctcc ggcaagtgag cccaaagcaa cttcaggtgg agtcccacca
     3061 ataaccgcag caagagaacc aacagtaaga tactgattta catgatcatt agttatttga
     3121 aatgacatat caaccaccat ttaacattca tcaaatctcc agtttctggt agaaca
//
LOCUS       DAZFAH010000314         3163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFAH010000314 DAZFAH010000000
VERSION     DAZFAH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.316"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..530
                     /locus_tag="ACHJWB_004729"
     CDS             <1..530
                     /locus_tag="ACHJWB_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFO4316202.1"
                     /translation="EKKRTGKISKIITSGGSFWKDNRFTVLSGGLIIAVLNSLVLAMH
                     GSAWTITGAIYDTSLHTASFLGVLSDNSVITKPLFQNPMVGMFVGGLLGAAMATVLTG
                     KRIKEPLTRGSIMAGITGGLLMGTGGAYASCNLGGFFDGTASGSLHGWLWMFMALAGS
                     VIGINLRRFFICSRC"
     gene            843..1235
                     /locus_tag="ACHJWB_004730"
     CDS             843..1235
                     /locus_tag="ACHJWB_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4316203.1"
                     /translation="MRDIQQVLERWGAWAANNYEDVTWSPIATGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            1394..2622
                     /locus_tag="ACHJWB_004731"
                     /pseudo
     CDS             1394..2622
                     /locus_tag="ACHJWB_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(2892..>3163)
                     /locus_tag="ACHJWB_004732"
     CDS             complement(2892..>3163)
                     /locus_tag="ACHJWB_004732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316204.1"
                     /translation="CGHYDHPYRNDDSRFAKGITKIIIPSAGGVRLRGLFVMLPTDST
                     RRRINPRGVLTLAEEKHESVVFTGAVSGLFLANHSLHTWLFPVNL"
BASE COUNT          879 a          666 c          865 g          753 t
ORIGIN      
        1 ttgagaaaaa acgcaccggg aaaatatcta aaattatcac cagtggtggt tctttctgga
       61 aagataacag atttactgtt ctttctggcg ggttaattat agctgtactg aactcactgg
      121 ttctggcaat gcacggttca gcatggacaa ttaccggagc catctacgat acatccttgc
      181 atactgcatc atttcttggg gttttatctg ataattctgt tattactaaa ccattatttc
      241 agaatcctat ggttgggatg ttcgtaggtg gattgctggg ggctgcaatg gctactgtac
      301 taacgggaaa aaggatcaaa gagcctctga ctcgtggaag cataatggca ggaataactg
      361 gtggattgct tatgggaaca ggaggagcgt atgcttcctg taatttggga ggattcttcg
      421 atggaactgc atcaggaagt ttacatggat ggttgtggat gtttatggct ctggctggca
      481 gtgtaattgg gataaatctt cggagatttt ttatttgttc acgctgttaa aaacaaataa
      541 ttgaatcctg acaggaaact gtgggaagaa ttctattatc agaaagtaaa gcccccttgt
      601 tttaaatgta tacaaggggg atgcatgctg aaaaagcgta ctatcaaaaa acagtaacta
      661 cgcgacgtag tactacctac tctagaaaaa ttagctgagc aaccgactat ctgaaatata
      721 ctcacaacct acgaaatttt cgtattttaa cctccgtccg gtatataccc cgaattacgg
      781 ggatgtacgg agttactgta cggagttacc gttgccaata attattcgca aggtgattgt
      841 tgatgcgtga tattcagcag gttctggaac gatggggggc gtgggcggca aataactatg
      901 aggatgttac atggtcgccc attgctaccg gatttaaggg actgatcccc gaaaaagtaa
      961 aatcacgtcc acagtgttgt gacgatgacg cgatgattat atgcgggtgt atggctcgcc
     1021 ttaacaggaa caacagcgat ctgcatgact tgctggttga ttattacgtg ttggggaaaa
     1081 cgtttatggc gctggcacgt aagcatggat gctctgatgg acatatcgga aagcggctac
     1141 agaaagcgga aggtgttgtt gaaggcatgc tgatgatgct ggatgtgaag ctggagatgg
     1201 atcggtatgt tgagcgcgat tcagtgacgg cataacgcgc ataaaaaaaa ccgcagtcat
     1261 gaggaaactg cggcggagaa gtcagacttg ccaatcaatc attagactgg ccccctgaat
     1321 ctccagacaa ccagtatcac ttaaataagt gatagtctta atactagttt ttagactagt
     1381 cattggagaa cagatgattg atgtcttagg gacggagaaa cgcagacggc gtaccacaca
     1441 ggaaaagatc gcaattgttc agcagagctt tgaaccgggg atgacggtct ccctcgttgc
     1501 ccggcaacat ggtgtggcag ccagccagtt atttctctgg cgtaagcaat accaggaagg
     1561 aagtcttact gctgtggccg ccggagaaca ggttgttcct gcctctgaac ttgctgccgc
     1621 catgaagcag attaaagaac tccagcgcct gctcggcaag aaaacgatgg aaaatgaact
     1681 cctcaaagaa gccgttgaat atggacgggc aaaaaagtgg atagcgcacg cgcccttatt
     1741 gcccggggat ggggagtaag cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg
     1801 cacgtcattc tcagacgaac cgatgactgg atggatggcc gccgcagtcg tcacactgat
     1861 gatacggatg tgcttctccg tatacaccat gttatcggag agctgcccac gtatggttat
     1921 cgtcgggtat gggcgctgct tcgcagacag gcagaacttg atggtatgcc tgcgatcaat
     1981 gccaaacgtg tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaacctgct
     2041 gtaccgccat cgaaacgggc acatacaggc agagtggccg tgaaagaaag caatcagcga
     2101 tggtgctctg acgggttcga gttctgctgt gataacggag agagactgcg tgtcacgttc
     2161 gcgctggact gctgtgatcg tgaggcactg cactgggcgg tgactaccgg cggcttcaac
     2221 agtgaaacag tacaggacgt catgctggga gcggtggaac gccgcttcgg caacgatctt
     2281 ccgtcgtctc cagtggagtg gctgacggat aatggttcat gctaccgggc taatgaaaca
     2341 cgccagttcg cccggatgta gggacttgaa ccgaagaaca cggcggtgcg gagtccggag
     2401 agtaacggaa tagcagagag cttcgtgaaa acgataaagc gtgactacat cagtatcatg
     2461 cccaaaccag acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac
     2521 gaatggcatc cgcatagtgc gctgggttat cgctcgccac gggaatatct gcggcagcgg
     2581 gcttgtaatg ggttaagtga taacagatgt ctggaaatat aggggcaaat ccaatcatga
     2641 aaaacgtaat cgcctgataa ttatgccacg aagcacggaa aaacagtact ggtgctaaga
     2701 catgaaccat ttgcattgca ataagttaag ccgaaatgcg tcgggtcctg aatggtgata
     2761 atcaccacgc agacaatcaa agctaccata acgaacatcg ttacagttgg agcttttacc
     2821 ccgttgggga aaaggtgcac atctggatca tcattatggt gatcgagggt atgtaaagcc
     2881 caccagcctg attaaaggtt aaccggaaaa agccaggtat ggagggagtg gttagccaag
     2941 aacaagccgg acaccgcgcc agtaaaaaca acagattcgt gcttttcttc agccagggta
     3001 agaactccac ggggattaat ccgtcgtctg gtggaatcag tcggcagcat gacgaacagg
     3061 ccgcgcagcc ggacaccgcc agcagaaggt attatgattt tcgtaatacc tttagcaaag
     3121 cgtgaatcat cattacggta gggatggtca taatgaccac acc
//
LOCUS       DAZFAH010000315         3157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFAH010000315 DAZFAH010000000
VERSION     DAZFAH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.317"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(533..898)
                     /gene="ylbG"
                     /locus_tag="ACHJWB_004733"
                     /pseudo
     CDS             complement(533..898)
                     /gene="ylbG"
                     /locus_tag="ACHJWB_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1014..2108)
                     /gene="mnmH"
                     /locus_tag="ACHJWB_004734"
     CDS             complement(1014..2108)
                     /gene="mnmH"
                     /locus_tag="ACHJWB_004734"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4316205.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            complement(2177..3103)
                     /gene="allS"
                     /locus_tag="ACHJWB_004735"
     CDS             complement(2177..3103)
                     /gene="allS"
                     /locus_tag="ACHJWB_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4316206.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT          783 a          830 c          758 g          786 t
ORIGIN      
        1 tgtgcagagt aattgcttga attaagagtc tatcccatat cgaagtcgtc aacttcgtag
       61 tgaggaaaag taaaattcat gactgagaaa agacatgtcg gctattgtgt aaagccatat
      121 agctcagacg atgaatatct actcgtattc agttgtttat tgagggtgag ttccgaccct
      181 gaaacaacaa ataaaatgaa cagtcagaga gtttacatag aattgcactg gtcttttacg
      241 atatctgaca ttgtgtaata catattcagc catgcattaa ttaaaatgtt acgtgtttaa
      301 tgtgagtccc tatctgaaaa taaataatcc ttccggatta aaataaattc ttgccgggaa
      361 agaaagagga aataaaccat tagcggaaaa ccagcggcac gccagggtga catgccccag
      421 tacggcggcc cgatagtcca gggttcagcg ggggtactga tagtcgcgcc gactgcatcg
      481 agaactccat acaacttttt acggaacgtc acctgataaa atttgttcag tatcgtcttt
      541 tgagattctc aaggttatgg agtaaccaga cagaacggac accactgtcg gagataaaaa
      601 ccccctgttt acgcagctcg ttgctggccc ggtgctgacc atgtgtcggg aaagcgacgg
      661 cgtaatcaac aacagcctgt tcagttgcct catcggtacg gttcttaagg ttaggagcgc
      721 ggcgactacg attaatctgc gcatccacac cgccttcagc gaccagttcg cggtaacgat
      781 aaaacgtatc acgcgaaacg cccatgattt tacaggcttt tgatacgttg ctgagttctt
      841 cagccagatt gagcaaaccg gctttgtgtt tgatgacggg attggcagta tgaagcatga
      901 gagttacctc ttgttttgga taaggattcg acactcatat caaaaccggt aactctcaac
      961 ctttcaaggc catgtgtcag atcaagtcgc gactaataca aatacgtccc tcattaccgc
     1021 gccttaaccc attccgccac ttccgcccac tcaccgcgaa agacaacttt ttccgctttt
     1081 ttctcaagct gatagcgata cattgggtcg taatattctt taagtaacgg caccagccag
     1141 gccagatgac cgtcggtgct gccggtggtg agttgcgttg tcagcgccgt atccagctgg
     1201 gcagcgagtt cgttatagcg ctgtaacccc agccgacgct taatcgccga aagtccgtga
     1261 tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc cataagcgtg ggtaaaatca
     1321 tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa gacggatctc aaacggatct
     1381 tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca gacattccgg caggtgattc
     1441 gaaccgatca tccggctttc gtcttccagc acccacaggc gcaaatcctg acgggcgtcg
     1501 gttttcagca tttcggcagc cagcaggttt tcaaaactcg cctggctaag ttgtggttgt
     1561 aacgtgcgac caaacgccga accgcgatgg cgcgccagcc cttccagatc aacaccgttc
     1621 ggctgttgct gcactaacag cgttttaccg ctgccagtac aaccgccaat cagcactatc
     1681 ggtttttgtg ccagttcaat agtcgcctga atcgcggtct ggcgcagtgc cttataaccg
     1741 ccttccacca gcggataatc aatccccgct gcatacaacc agctttgcac aatatgtgag
     1801 cgctgaccgc cacgggcgca gcagagaata ccttgcggat tttgcaggca cgctgcccgc
     1861 caggcgtcca tgcgctgctg acgaatttca cccgccacca gtttatgtcc cagcgccagc
     1921 gctgcgtctg agccttgctg tttatagcag gtgccaacgg cggcgcgttc atcgttattc
     1981 attaacggca gattgatagc ggcgggcatt gcgccttgct caaactcgat aggggcgcga
     2041 acatcaataa tgggcgtatc agcaatcagc agggcacgat agtcctgttc cgtgtgtctc
     2101 tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg ctattttacg cgccaacgcg
     2161 caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat ttctaaaaat ccgctgattt
     2221 ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac cgctttgccg ctgccaaatt
     2281 tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt tgggattacc cggctaacca
     2341 gttgttgatt atcgatcatt gactggcaaa gcgattttgg caaaaaacca atgccaacgc
     2401 ccgcaagatg ggcggcgatt ttcgtttcca tatcaggaac aataatctct ttttgccctg
     2461 gcaatcgcca ggcgacgcgt ttggttaagg tgcgggcgct gtcttcaata ttgaccgccg
     2521 gaaagcgccg caactgcgct tctgttagcg gctcttcaac gttcgccagc ggatgatccg
     2581 ccgccatgac aaagcgccat tgcaccgatc ctaagggatc aagactaaag gtatttgcca
     2641 gcgcctcggt tcccgtgacg ccgatagcca gtgaaaaacc ttcgtacaat agcgaatccc
     2701 agacgcccat atagatttgt cgggagatgt gaaactgggt aaaggggtaa cgttcattca
     2761 gccacgccag caactgggcg acggcctggg ggttgtagag caggttgttg atgacaatat
     2821 tcacctggcg ttccacgcca tcattcacct gttgcagctc gcttggcata ctttccagcc
     2881 agctcagcca gtctctggcc tgggaaagta gatgctcgcc agccgctgtc aacgtcacgc
     2941 tacgagtcgt acggaaaaac agcgctactc cggtattctc ttccagaagt ttaatgcgat
     3001 aactgatcgt cgccgtggtt ttacataatc gttctgccgc ttttgaaaaa cttcctgttt
     3061 cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa catcttcagg tatccccttt
     3121 taaatccgca agttgcgtga ttttcttatc ctctgat
//
LOCUS       DAZFAH010000316         3135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFAH010000316 DAZFAH010000000
VERSION     DAZFAH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.318"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..1785
                     /gene="ydeN"
                     /locus_tag="ACHJWB_004736"
     CDS             103..1785
                     /gene="ydeN"
                     /locus_tag="ACHJWB_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4316207.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1837..2994
                     /gene="ydeM"
                     /locus_tag="ACHJWB_004737"
     CDS             1837..2994
                     /gene="ydeM"
                     /locus_tag="ACHJWB_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4316208.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKFELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2960..3082)
                     /locus_tag="ACHJWB_004738"
     CDS             complement(2960..3082)
                     /locus_tag="ACHJWB_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316209.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          956 a          677 c          627 g          875 t
ORIGIN      
        1 gctaaatctg ctatctctta atgtgactta cttcacttac atccacttat ttctcttcgt
       61 aaaattactt cggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag
      121 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
      181 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
      241 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact aaccatggat
      301 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
      361 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa
      421 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      481 gtggcacacg gtgtttccgg cccctcccgt gccgcaataa tgaccggtcg agctcccgcc
      541 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
      601 ctgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      661 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
      721 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
      781 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
      841 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
      901 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccacac
      961 ctgccaaatg ataatcctgc accggatcaa tatcagaaac aatttaatac cggtagtcaa
     1021 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
     1081 gaacaactga agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat
     1141 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     1201 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1261 ggtaattatg acaagctgat ttccgcaatg gatttctacc cgacagcact tgatgcagcc
     1321 gatatcagca ttccaaaaga ccttaagctg gatggtgttt ccttgctgcc ctggttgcaa
     1381 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1441 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     1501 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1561 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1621 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1681 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga agtaaatcag
     1741 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1801 gcggaatttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     1861 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     1921 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca
     1981 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     2041 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     2101 aatgcattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     2161 catgaatttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     2221 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     2281 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct
     2341 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2401 ctagaaaccg ggacgccgaa tattgatttc agtggccata gtgagaacac attccgtatc
     2461 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2521 tgggttaaaa acgatgtggg tgaaattttc attcgtcagt ttgaaagctt tgtcagccgt
     2581 tttttgggga atgggcatac aagttgtatt ttccaggagt cctgcaagga taatctggtt
     2641 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     2701 ggaaacatta ataaatttga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2761 aaacggattt cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggcggttgt
     2821 cctaagcatc gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat
     2881 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggttgagtt agctaagaac
     2941 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     3001 tctcttttaa ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa
     3061 ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga aaatcctggc ttacgtggta
     3121 tgcgtcaggg catta
//
LOCUS       DAZFAH010000317         3089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFAH010000317 DAZFAH010000000
VERSION     DAZFAH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.319"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            212..1443
                     /locus_tag="ACHJWB_004739"
                     /pseudo
     CDS             212..1443
                     /locus_tag="ACHJWB_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1428..2066
                     /locus_tag="ACHJWB_004740"
     CDS             1428..2066
                     /locus_tag="ACHJWB_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO4316210.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2145..2414
                     /locus_tag="ACHJWB_004741"
     CDS             2145..2414
                     /locus_tag="ACHJWB_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4316211.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2435..3079
                     /locus_tag="ACHJWB_004742"
     CDS             2435..3079
                     /locus_tag="ACHJWB_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316212.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
BASE COUNT          819 a          638 c          853 g          779 t
ORIGIN      
        1 caagttgaaa ggatggtata tgttgaaaat gcatggattt tccagatgtg ttaccagtaa
       61 gaagcaggtg gataaaggat gtgataacaa atccaacaac cagattaata tctgaagagt
      121 tttattgtta ttatttatcc ccaaagcggt gagttattaa tgaagtttgt ctgaatgcat
      181 aaacagaatt attcgacata aagccaggaa aatgagtgat acgcttttaa ccgaaaaaat
      241 ccttaccgga gaaaatgttt tgcgtgccgc aatcgcccgc attgagtgga tttttgaaac
      301 atttccgtcg gtatgcctgt ctttttcagg tggaaaggat tccactgtgc ttttccatct
      361 tgtggcagag gtggcccgca ggagaaaacg tcatttctct gttctgttca ttgactggga
      421 ggcccagtat cggtgcacta ttgaacatat tcagaagatg cgggaaatgt accatgatgt
      481 gacggaaacc ttttactggg tggcacttcc ctgaccacgg taaacggcgt ctcgcagttt
      541 cagccggagt ggatatgctg ggagccagga gtgacctggg ttcgccagcc accggaagag
      601 gccattacag acatgacgta ttttcccttt taccggtatg ccatgacgtt tgaagaattt
      661 gttccggcat tttcttcctg gtttgccggt aaccggtgtg gagtggcaat actgactggt
      721 gtccgtgctg atgaatcgct caatcgcttt atgggactgg tatctcagcg caaactgaga
      781 tatgccgatg ataagccctg gaccacggca tcgcctgaag ggttttatta caccatgtat
      841 ccgttgtatg actggaaggc ccgcgatatc tggatatata acgccagaac ctgtgccatt
      901 tacaatcctc tgtatgacct gatgtaccgt gccggcgtgc cgttacgcaa catgcgtgtc
      961 tgtgaacctt ttggtcctga acagcgtaag ggactgtggc tttaccatgt tctggagccg
     1021 gaaacctggg ccaggatgtg tgaacgggtg tcaggggctg ccagcggggc gctttatgcc
     1081 aatgagagtg gtgcctattt tgccctgtgt aagcgtatca cgaagccgcc tcatcatacc
     1141 tggcgcagct atgcgatgtt cctgctggat gtgatgccgg aaagaacagc agaacattac
     1201 cgtaataaaa ttgctgtcta cctgcgttgg tatcagacgc ggggcttccc ggatgacatt
     1261 ccggatgaac aggagaatga cctggggagc cgggatattc cgtcctggcg acgtatctgt
     1321 aagactctca taaagaatga tttctggtgc cggaccctct cctttagccc taacaaaccc
     1381 cggcactatg aacgttacct gcagcgtatg aaggaaagga ggaaggaatg ggggattctg
     1441 tgacaccgga gatacttggc aacatgattc gtcagtactt cagccaggaa cgaccagaag
     1501 aagaggccat acaggccctt aatcacctgc gcagggtgct tcatgaggtc agtccttttg
     1561 cacaggagcc tgtggactgt gtgctgtggg taaaagcaga tgaggtggtg gccaatgatt
     1621 acaatcctaa tgtcatggcg ccgggtgaga agaaactgct gaagcagtca ctggagaaag
     1681 atggtttcac acaaccggtg gtagtgtctg aggaaaagaa ccattacttg gtggtggatg
     1741 gttttcatcg ccaggtactg ggcagggaag caggtacggg aaaacgtctg aagggctggc
     1801 tgccggtggt ctgtattaat ccggagagaa agggacaggc ctcacgtatt gctgcgacca
     1861 tccgacataa ccgggccagg ggcaaacatc agataacctc aatgtctgat attgtcaggg
     1921 acctgtcgcg cctgggctgg acgaatgaac gcattggtac ggagctgggt atggaccagg
     1981 atgaagttct cagattgaaa cagattagcg ggctgacgga gctgtttcag gaagaggatt
     2041 tcagcccggc atggacggtt cgctgagacc gtcagatatg tatattattt cagtgagtta
     2101 atgttttgag cgggaaaaag agtaccccgg aagttgtgga agacatggtt gcgcagaaac
     2161 tggaggctgc tggttgctgg cgcagagctt ctgaccgctg gctttttgtt atgggaaatg
     2221 ttgaatgtac agaagcacaa cgggagtggt tgttgttgcg ccggaattat tgtctggcgc
     2281 agatatcgtc cccgccgcta cctgaaacgc tggatatcag cgaggtggcg aaagctgcag
     2341 atgccacgct gaggcgaatg gggattgcca ctccgtccgg ggaggtattc agaaaaggta
     2401 caccggtctg ttgatattca ggtaacaggt attaatgatg aagcaaggaa tggcaaacag
     2461 tatatcaatg acatggcaac ttttatgcga tgaacatggt tttacccggg agcactacaa
     2521 tcagctgaag aagttcagcc cggagacatt acgtgaaatt attgctgaaa tagccagctg
     2581 tcatccatca accagtgtat tgctcaggaa taagtggctg acaccgccgg aggatattct
     2641 ggagcagatt acccgtgaat acgaacgaag aatacaaaac tgtccaccgt tcaggagcga
     2701 gaaggaagcg gaatcctggt taaatgaact atcttttgct gtgattgctc cgctataccg
     2761 tgttgccccc gaacaaacag aacaggttga gtcctttgtt ctgcagctta ttgccgagca
     2821 ggagcgggtt tgggagctta ttttaaccaa tgatggttat tcatggcatt gcgcacttta
     2881 tgatatatta tttcatctgg ttttacgaaa tatggaagac cagcctgaac atcagaaaca
     2941 gcgaataaca aaaatatttt atgagcccga tgcaagactg gttgcagaag agattaaatt
     3001 ccggattcaa tctctgtatg atgatgaaca aaagagagct ttaagtgaac tggtaaatga
     3061 ttttacttca aaagaataaa atgcggcga
//
LOCUS       DAZFAH010000318         3087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFAH010000318 DAZFAH010000000
VERSION     DAZFAH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.320"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..1641
                     /gene="ydiF"
                     /locus_tag="ACHJWB_004743"
     CDS             46..1641
                     /gene="ydiF"
                     /locus_tag="ACHJWB_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO4316213.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1655..2806
                     /gene="ydiO"
                     /locus_tag="ACHJWB_004744"
     CDS             1655..2806
                     /gene="ydiO"
                     /locus_tag="ACHJWB_004744"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4316214.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2849..>3087)
                     /locus_tag="ACHJWB_004745"
     CDS             complement(2849..>3087)
                     /locus_tag="ACHJWB_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316215.1"
                     /translation="INYVIQRRMMEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLR
                     HVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
BASE COUNT          829 a          710 c          792 g          756 t
ORIGIN      
        1 ctccatctgg cgtagggaaa atcacatctg aatcaggaat taacaatgaa acctgtaaaa
       61 ccacctcgta ttaatggacg agtgccggtc ctgtcggcac aggaagcggt gaattatatt
      121 cccgacgaag caacactttg tgtgttaggc gctggcggcg gtattctgga agccaccacg
      181 ttaattacag ctcttgctga taaatataaa cagactcaaa cgccacgtaa tttatcgatt
      241 attagtccga cagggcttgg cgatcgcgcc gaccgtggta ttagtcctct ggcgcaagaa
      301 ggtctggtga aatgggcatt atgtggtcac tggggacaat cgccgcgtat ttctgaactc
      361 gcagaacaaa ataaaattat tgcttataac tatccacaag gtgtacttac acaaacctta
      421 cgcgccgccg cagcccacca gcctggtatt attagtgata ttggcatcgg gacatttgtc
      481 gatccacgcc agcaaggcgg caaactgaat gaagtcacga aagaagacct gattaaactg
      541 gtcgagtttg ataacaaaga atatctctat tacaaagcga ttgcgccaga tatcgccttc
      601 attcgcgcta ccacctgcga cagtgaaggc tacgccactt ttgaagatga ggtgatgtat
      661 ctcgacgcat tggttattgc ccaggcggtg cacaataacg gcggtattgt gatgatgcag
      721 gtgcagaaaa tggttaagaa agccacgctg catcctaaat ctgtccgtat tccgggttat
      781 ctggtggata ttgtggtggt cgatccggat caaagccaac tgtatggcgg tgcgccggtt
      841 aaccgcttta tttctggtga cttcaccctt gatgacagca ccaaacttag cctgccccta
      901 aaccaacgta aattagttgc gaggcgtgca ttattcgaaa tgcgtaaagg cgcggtgggg
      961 aatgtcggcg tcggtattgc tgacggtatt ggcctggtcg cccgggaaga aggttgtgct
     1021 gatgacttta ttctgacggt agaaacaggt ccgattggcg gaattacttc acaggggatc
     1081 gcctttggcg cgaacgtgaa tacccgtgcc attctggata tgacgtccca gtttgatttt
     1141 tatcacggtg gtggtctgga tgtttgttat ttgagttttg ctgaagtcga ccagcacggt
     1201 aacgtcggcg tgcataaatt caatggcaaa atcatgggta ccggtggatt tattgatatc
     1261 agtgccacct cgaagaaaat tattttttgc ggcacgttaa ctgcgggcag tttaaaaaca
     1321 gaaattgccg atggcaaatt gcatatcgtc caggaaggac gggtaaataa atttattcgg
     1381 gaactaccgg aaattacttt tagcggaaaa atcgcgctcg aacggggact ggatgttcgt
     1441 tacatcaccg aacgtgctgt attcacgctg aaagaagacg gcctgcattt aatcgaaatc
     1501 gcccctggcg tcgatttaca aaaagatatt ctcgacaaaa tggacttcac cccagtgatt
     1561 tcgccagaac tcaaactgat ggacgaaaga ttatttatcg atgcggcgat gggttttgtc
     1621 ctgcctgaag cggctcatta acaggagtat aatgatggat ttttctttaa ctgaagaaca
     1681 agaactgctg ctggccagta ttcgcgaact gattacgact aactttccgg aagagtattt
     1741 ccgcacctgc gatcaaaacg ggacatatcc gcgtgagttt atgcgggcgc tggcggataa
     1801 cggtatttcc atgcttggcg tgccggaaga atttggtggt atccctgcgg attacgtcac
     1861 ccaaatgctg gcgctgatgg aagtgtcaaa atgcggtgct ccggcatttt taattaccaa
     1921 cggtcaatgt attcacagta tgcgccgttt cggttctgca gagcagctac gtaaaacggc
     1981 agagagcacc ctggaaacgg gtgatcccgc ctatgccctg gcgttgacgg aaccaggtgc
     2041 tggctcagat aacaacagtg ccactaccac ttacacgcgt aaaaacggca aggtctacat
     2101 caacggacaa aaaaccttta ttaccggtgc gaaagagtac ccgtatatgc tggtgctggc
     2161 gcgcgatccg caaccgaaag atcccaaaaa agccttcacc ctgtggtggg tcgactccag
     2221 taagcccggc attaagatta acccgctgca taaaatcggc tggcatatgc tcagcacctg
     2281 cgaagtctat ctcgacaatg tggaagttga agagagcgac atggtgggcg aagaaggaat
     2341 gggtttcctc aatgtgatgt acaactttga gatggagcgc ctgatcaacg ccgcgcgcag
     2401 caccggcttt gccgaatgcg cctttgaaga tgccgcccgc tatgccaacc aacgtatcgc
     2461 ttttggtaag cccattggtc ataaccagat gatccaggaa aaactggcgc tgatggcgat
     2521 taagatcgac aacatgcgca acatggtgct gaaagtggca tggcaagccg atcagcatca
     2581 gtcactgcgc accagcgcgg cgctggcaaa actgtattgc gcacgtaccg caatggaagt
     2641 cattgatgat gcgattcaaa tcatgggcgg tctgggctat accgatgaag cgcgcgtctc
     2701 ccgcttctgg cgtgatgtcc gttgtgaacg tatcggcggc ggtacagacg aaattatgat
     2761 ttacgtagca ggtcggcaga tcctgaaaga ctatcagaac aaataatctg caggcggcgc
     2821 agcttcttaa caaactgcgc cgccagattt atccaacaag acttaccggt tgaggaaatt
     2881 cagataggat ttcttgttcc gcaaaacagt ttttaaattg tctacggtaa tcgctgggcg
     2941 aacaaccgac atggcgcaaa aacagtttgg caaagtgatc gacattttca taacccacac
     3001 gccaggagat ctctgcctgt gataattcag tattagtgag tgaccatttt gcttccatca
     3061 tacgccgttg aataacatag ttaatag
//
LOCUS       DAZFAH010000319         3051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFAH010000319 DAZFAH010000000
VERSION     DAZFAH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.321"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..1853
                     /gene="espL1"
                     /locus_tag="ACHJWB_004746"
                     /pseudo
     CDS             2..1853
                     /gene="espL1"
                     /locus_tag="ACHJWB_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2025..2132
                     /gene="yniD"
                     /locus_tag="ACHJWB_004747"
     CDS             2025..2132
                     /gene="yniD"
                     /locus_tag="ACHJWB_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO4316216.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(2185..2943)
                     /locus_tag="ACHJWB_004748"
     CDS             complement(2185..2943)
                     /locus_tag="ACHJWB_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4316217.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          917 a          695 c          601 g          838 t
ORIGIN      
        1 cattatcaga actcattata agtctcctca tagattgcac atcgatagcg acataccaac
       61 accttcatca gagcctatta ataaatttgc gcgccagctc atcaccctac ttgatacctc
      121 tgacttaagt tcgatgctgt catactgtgt tattcaggaa tttaccgcaa actgtcgaaa
      181 aatatcacaa aattgttatt ccactgccct ttttaccatt aactttgcca cttcacccat
      241 ccatgcagaa aatatactca ttacattaca ctataaaaaa gatttcattt ccttattact
      301 gaaaaccacg cctattaaag ctaaccattt gcgaagcata ctggattata ttgaacagga
      361 acagttaact gctgaagatc gtaaccattg tatgaaactg tctaaaaaaa tccatagaga
      421 aaaaactata cacccaacag taaatctcaa tggtagtgca tgttttttgc aatctccttc
      481 tgacgctatt ttttgtcgcc atctgtcatt gcaatacgcc cttgattcat tgagaaatgg
      541 aaaaggcaaa gtcaacctga ttaaacatta ctcctccgtt gaatccatac agcagcatgt
      601 ccccttagtc cgggacgcgg agttcagagc attacttcgc catcctcctg cagggagtcg
      661 cgttatcgcg agtaaggatt ttggcttcgc tttagatatt ttcttttgtc gaatgatggc
      721 aaacaatgtc agtcatatgt ccgcgatttt atatatagac aatcatactt tgtcagtaag
      781 gctacgaata aagcagtcag tgtatggaca attaaactat gttgtgtccg tttacgaccc
      841 gaacgatacc aacgttgccg tcagagacac ccacaggaca gcacgggcct ttctttcgct
      901 tgataagttc atcagttcag gtcccgatgc tcagacctgg gctgataggt atgttcgcaa
      961 ctgtgcaatt gctattctgc ccctattacc tgtgggagtt ccaggggcta ttttcgcggg
     1021 tattgcatca cgaatgccat ttgcccctat acatccatcg gcaatgttgt taataatggc
     1081 tacaggccag actcaacagc ttattacatt attcaaacag ttgcccatac tccctgaaaa
     1141 agaaatcatt gaaataataa ctgcgcagaa tagcgttggt acacctgctt tatttttggc
     1201 tatgatgaac ggacatactg acaacgtgaa aatatttatg caagaaattc agtcactggt
     1261 agataatcac atcattcatg aagataatct ggttaaatta ctgcaaacta aaagtgctaa
     1321 cgaaacacct ggactttata tctccatgtt gtatggattc gatgaaataa tcgatatctt
     1381 tctgaatgca ttaaccactc caatagcaca agagctttta aacaaaaaac tggtgatgag
     1441 tattttagcc atgaaaatac atgatggtga gccaggatta tacgccgcaa tggaaaataa
     1501 tcaccctttg tgtgtcacac ggttcctctc taaaattaat ggcatcgcct ttaaatacaa
     1561 gttgagcaaa gctagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg
     1621 aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta
     1681 gtcaacatca gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc
     1741 atatagccat tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg
     1801 tcatcagcca aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta
     1861 tttacgatat atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca
     1921 ggtgtacaac gccacccaac ggctgtaata acaaaaaatc ccttgatgcc cgtccctttt
     1981 gttacactcc gttatcacgc acaagaaata tgcaggacac tggtatgccg actaaacgct
     2041 ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt
     2101 tgctacgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg
     2161 cataacacgg tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg
     2221 atcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac
     2281 cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt
     2341 atcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca
     2401 tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt
     2461 gtcgtaacgc acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa
     2521 ctggcgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt
     2581 cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc
     2641 gcccgccgca tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc
     2701 ccacagcgac caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga
     2761 ggagcttttc gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg
     2821 ttttttagcg ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt
     2881 cagtgacgca aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt
     2941 catttatcaa gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta
     3001 aggatcaaag attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc g
//
LOCUS       DAZFAH010000320         3047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFAH010000320 DAZFAH010000000
VERSION     DAZFAH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.322"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHJWB_004749"
     CDS             complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHJWB_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4316218.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2091..2957
                     /gene="yghU"
                     /locus_tag="ACHJWB_004750"
     CDS             2091..2957
                     /gene="yghU"
                     /locus_tag="ACHJWB_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4316219.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDATEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          719 a          819 c          773 g          736 t
ORIGIN      
        1 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
       61 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      121 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      181 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      241 tgatggctga ccaggtcgat attgctacca cagcgaccgg caatcggttt cactgcgtaa
      301 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      361 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      421 ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      481 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      541 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg cccttcccca
      601 tcaatcagtt gcccggcagc atcccagccc agttcatcca atccacgcaa gatacgcgtt
      661 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      721 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      781 aattcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      841 caacgttcga ggatcaagcc agcttcggta tgacaggagg cggagtcggc gttgtattcg
      901 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
      961 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1021 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1081 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1141 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1201 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1261 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1321 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1381 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1441 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1501 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1561 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1621 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1681 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1741 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1801 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1861 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1921 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     1981 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2041 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2101 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2161 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2221 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2281 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2341 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2401 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2461 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2521 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2581 tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2641 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt gcgggcgatg
     2701 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg ttaggcggcg
     2761 tgtatgatgc cacagagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2821 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2881 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     2941 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3001 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctg
//
LOCUS       DAZFAH010000321         3041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFAH010000321 DAZFAH010000000
VERSION     DAZFAH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.323"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..837)
                     /gene="ycjM"
                     /locus_tag="ACHJWB_004751"
     CDS             complement(<1..837)
                     /gene="ycjM"
                     /locus_tag="ACHJWB_004751"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO4316220.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDN"
     gene            complement(1049..1363)
                     /gene="pspE"
                     /locus_tag="ACHJWB_004752"
     CDS             complement(1049..1363)
                     /gene="pspE"
                     /locus_tag="ACHJWB_004752"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4316221.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1438..1659)
                     /gene="pspD"
                     /locus_tag="ACHJWB_004753"
     CDS             complement(1438..1659)
                     /gene="pspD"
                     /locus_tag="ACHJWB_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4316222.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1668..2027)
                     /gene="pspC"
                     /locus_tag="ACHJWB_004754"
     CDS             complement(1668..2027)
                     /gene="pspC"
                     /locus_tag="ACHJWB_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4316223.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2027..2251)
                     /gene="pspB"
                     /locus_tag="ACHJWB_004755"
     CDS             complement(2027..2251)
                     /gene="pspB"
                     /locus_tag="ACHJWB_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4316224.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2302..2973)
                     /gene="pspA"
                     /locus_tag="ACHJWB_004756"
     CDS             complement(2302..2973)
                     /gene="pspA"
                     /locus_tag="ACHJWB_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4316225.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQQ"
BASE COUNT          769 a          768 c          675 g          829 t
ORIGIN      
        1 gttgtcttta tgcggaacat tggtctcggt aatgatcact gtacctggcg caacgttatc
       61 aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga tgcagcttgt
      121 tcccggctct ttccacataa agccaacggc atccaggcgg acatactcag cacctttcgc
      181 aaggtaacag agtaaaacat ccaccatcgc cagcaacact tcagggctac ggtaattcag
      241 gtcaatttga tcgtcactaa aggtggtcca taaatggcgc gttgaatgat cgcgcatctg
      301 gaatggcgtt aataacggta acgcacgcgg acgggtgacg gcgctgagat cggtttgcgg
      361 atcaaccgca ataaaaaaat cttcaaaacc tggctgctgt tgtaaatagt ttttaaacca
      421 ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc
      481 gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac
      541 cgaaaagcca tcatcagatg accatggata aaacggcaac aaatgaacat gtgaaaaaat
      601 gctttgcagc cattgatggt aaaactgatt aaatgtgggt aatggtttta aatcattgct
      661 gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg
      721 tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt
      781 tgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct gtttcatgtt
      841 gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcagaaaa taaactgtca
      901 attgagcaca agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt
      961 tgtgagctga cgcaaataat ctatggggat gtaaataagg tgtgacagaa aaaaaacggc
     1021 gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt
     1081 tcaggccacc ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgctt
     1141 gccctgactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg
     1201 cggtggcaat gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct
     1261 cttgctgata ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca
     1321 gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg
     1381 gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca
     1441 ccttttataa cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac
     1501 agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgctacacc
     1561 cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccagg
     1621 ctttaccttt tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg
     1681 aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc
     1741 gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc
     1801 tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa
     1861 gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag gatacgcacc
     1921 agttttaccg gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc
     1981 tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc
     2041 agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt
     2101 ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac
     2161 cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg
     2221 ttaacggaat agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg
     2281 cgttcggacg ccgcctggat gttattgttg attgtcttgc ttcattttgg cttttaattg
     2341 tgccagttgt tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga
     2401 tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc
     2461 gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac
     2521 atcgcgcgac gagtttgccg cctgatgacg taacatcaat gcctgctggc gagcgcgtgt
     2581 ttcgctcaat ttgttttcca gctcgccaat ctctttcttc atgcgtgcca gcgtatcgtc
     2641 caccagcgtc acttcatgtt ccagggactt aatcagatcg gtcagtttct gtttttcaat
     2701 taacgctgca cgtgccagat cctctctctc tttcagcagc gccagttcgg ctttttcctg
     2761 ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc
     2821 caacgcgcgc gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat
     2881 cagacgaacc agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt
     2941 cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc
     3001 aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca a
//
LOCUS       DAZFAH010000322         3000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFAH010000322 DAZFAH010000000
VERSION     DAZFAH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.324"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            100..1389
                     /gene="hpaG"
                     /locus_tag="ACHJWB_004757"
     CDS             100..1389
                     /gene="hpaG"
                     /locus_tag="ACHJWB_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO4316226.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1386..2852
                     /gene="hpaE"
                     /locus_tag="ACHJWB_004758"
     CDS             1386..2852
                     /gene="hpaE"
                     /locus_tag="ACHJWB_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4316227.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHREKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2854..>3000
                     /locus_tag="ACHJWB_004759"
     CDS             2854..>3000
                     /locus_tag="ACHJWB_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO4316228.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVD"
BASE COUNT          719 a          838 c          861 g          582 t
ORIGIN      
        1 tcacatttac atcacttact tttgccaaac tacgcaatcg ggaataaaag taatcattaa
       61 catattaatg attaaaatga tcgaatccga ggagtggtaa tgaaaggcac tatcttcgcc
      121 gtagcgttga accatcgcag ccaacttgat gcatggcagg aagcgttcca gcaatccccc
      181 tacaaagccc cgcctaaaac tgcggtctgg tttattaaac cgcgcaatac ggtgattggt
      241 tgcggtgaac cgattccctt tccacagggt gaaaaggtac tgagcggtgc gactgttgcg
      301 ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag atgcggcaga gtacatcgcc
      361 ggatatgcgc tggctaacga cgtcagcctg ccggaagaga gcttttaccg cccggcaatc
      421 aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa ccgtggctct cagcaatgtc
      481 gataatctga ccatctatac cgagatcaac gggcgtcctg ccgatcactg gaataccgcc
      541 gatttacaac gtaacgccgc gcagttgctg agcgccctga gcgaatttgc cacgctgaat
      601 ccaggcgacg ccattctgct cggcacgcca caggcgcgcg tggaaataca gccaggcgat
      661 cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa atccggtagt ggacgaacgt
      721 gaagtgacca cgcgcaagag cttcccaacg cagccacacc cgcacggcac gctgtttgcc
      781 ctcggcctga actacgccga ccacgccagc gaactggaat ttaagccacc ggaagaaccg
      841 ctggtgttcc tgaaagcgcc aaataccctc actggcgata accagacctc cgtgcgtcca
      901 aacaatattg aatacatgca ctatgaagcg gagctggtgg tagttattgg caaacaggcg
      961 cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg gctacaccgt gtgtaacgac
     1021 tacgccattc gcgactatct ggaaaactac taccgcccta acctgcgggt caaaagccgc
     1081 gacggactga cgccgatgct ttcaaccatc gtgccgaaag aggcgatccc ggacccgcat
     1141 aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc agcaaggcac caccgccgat
     1201 ctgatcttca gcgtgccctt cctgatcgcc tacttaagcg aatttatgac cctgaatccg
     1261 ggcgacatga tcgccaccgg cacaccaaaa ggcttatctg acgtggtgcc tggcgatgaa
     1321 gtggtggtgg aagtagaagg cgtgggccgc ctggtgaacc gaattgtgag tgaggaaaca
     1381 gcgaaatgaa aaaagtaaat cattggatca acggtaaaaa tgttgcaggt aacgactact
     1441 tccagactac caatccggca acgggtgaag tgctggcgga tgtggcctcc ggcggtgaag
     1501 cggagatcaa tcaggcggta gcggcagcga aagaggcgtt cccgaaatgg gccaatctgc
     1561 cgatgaaaga gcgcgcgcgc ctgatgcgcc gtctgggcga tctaatcgac cagaacgtgc
     1621 cggagatcgc cgcgatggaa accgcggaca cgggcctgcc gatccatcag accaaaaatg
     1681 tgttgatccc acgcgcttct cacaactttg aatttttcgc ggaagtctgc cagcagatga
     1741 acggcaagac ttatccggtc gacgacaaga tgctcaacta cacgctggtg cagccggtag
     1801 gcgtttgtgc actggtgtca ccgtggaacg tgccgtttat gaccgccacc tggaaggtcg
     1861 cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat gtcggaactc tccccgctga
     1921 ccgccgaccg cctgggtgag ctggcgctgg aagctggtat tccggcgggc gtgctgaacg
     1981 tggtacaggg ctacggcgca accgcaggcg atgcgctggt ccgtcatcat gacgtgcgtg
     2041 ccgtgtcgtt caccggcggt acggcgaccg ggcgcaacat catgaaaaac gccgggctga
     2101 aaaaatactc catggaactg ggcggtaaat cgccggtgct gatttttgaa gatgctgata
     2161 ttgaacgcgc gctggacgcc gccctgttca ccatcttctc gatcaacggc gaacgctgca
     2221 ccgccggttc gcgcatcttt attcagcaaa gcatctaccc ggaattcgtt aaacgctttg
     2281 ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga tccgaatacc caggttggcg
     2341 cgcttatcag ccagcagcac cgggaaaaag tctccggcta tatccgtctc ggcattgaag
     2401 aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc cgacctgcct gcgcacctga
     2461 aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt tgataaccgt atgcgcgtcg
     2521 cccaggaaga gattttcggg ccggtcgcct gcctgctgcc gtttaaagac gaagctgaag
     2581 gcttacgcct ggcaaacgac gtggagtacg gcctcgcgtc gtacatctgg actcaggatg
     2641 tcagcaaagt gctgcgtctg gcgcgcggga ttgaagcggg catggtgttc gtcaacaccc
     2701 agaacgtgcg tgatttgcgc cagccgtttg gcggcgtaaa agcctccggc accgggcgtg
     2761 aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa gaacgtctgc atttccatgg
     2821 gcgaccatcc aattccgaaa tggggagtct gatatgggta agttagcgtt agccgccaag
     2881 atcacgcacg taccgtcgat gtatctctct gaactgccag ggaaaaacca cggttgccgc
     2941 cagggcgcga tcgacgggca taaagagatc agcaagcgtt gccgggaaat gggcgtcgat
//
LOCUS       DAZFAH010000323         2995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFAH010000323 DAZFAH010000000
VERSION     DAZFAH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.325"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..686
                     /locus_tag="ACHJWB_004760"
     CDS             <1..686
                     /locus_tag="ACHJWB_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316229.1"
                     /translation="YTWKFTTIISDESDTIENTTAWEKDYCQITIQRDELLKLIYTNT
                     LQDAAMNLAGCLQQAYIEPLKREINYPGINIDAKEREIRKSIEFRSELNNFTNIMKQL
                     ETFEKWYNANKILLVSNKKKRTEKIDVNIRLAGLKGYDLNEGIPDGQGRKIKDGVNEL
                     VKADGSLQLPQDISDTSLNRYRQAVQHIIKDEIDTLLIAQREKNKINPYSEDRYSIRP
                     LWGKCLVEQ"
     gene            complement(769..1365)
                     /locus_tag="ACHJWB_004761"
     CDS             complement(769..1365)
                     /locus_tag="ACHJWB_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316230.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDVRHKVVATLELQRRPRLEGVFVQKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETIVTKAGVVLLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGE
                     RISYDGKSIPESEIWSEHPERNRHAVVLVAESVNGKAA"
     gene            complement(1495..2811)
                     /locus_tag="ACHJWB_004762"
     CDS             complement(1495..2811)
                     /locus_tag="ACHJWB_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4316231.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGKKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQAELERQQFAE
                     QQRIQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSAGITFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRFCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRAFNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
BASE COUNT          940 a          675 c          589 g          791 t
ORIGIN      
        1 gttacacctg gaaattcaca acaattattt ccgatgaaag tgacacgatt gaaaacacca
       61 ccgcatggga aaaagactat tgtcaaatca ctatacagcg cgatgaactt ctgaaactga
      121 tttataccaa taccctgcag gatgcagcaa tgaatcttgc tggttgcctg caacaggcat
      181 acatagaacc gttaaaaagg gaaataaatt atccaggaat aaacattgat gcaaaagagc
      241 gtgaaattag aaaatctatt gaattcagga gcgaactgaa taacttcaca aacatcatga
      301 aacaactgga aacatttgaa aaatggtata atgccaataa aattctgttg gtaagtaaca
      361 aaaagaaacg tacagaaaag attgacgtga atattcgtct tgcaggactg aaaggctatg
      421 atctgaatga aggtatacct gatggtcagg gacgcaaaat taaagatggt gtgaatgaac
      481 tcgtcaaggc agatggcagt ctacaacttc cacaagatat cagtgacact tcgcttaacc
      541 gttaccgtca agcagttcaa cacattatta aggatgagat cgacacgttg ctgatagcac
      601 agagggaaaa aaataaaata aatccatata gcgaagatag atactcgata cgacctttat
      661 ggggaaaatg tctggtcgaa cagtaactca ctgagaaagg ggatagcagt tcgctatccc
      721 caagggagac tttccagcaa gatctcaaaa aacgagacac tcggcaaatt atgcggcttt
      781 accattaaca gattcagcca caagaacgac agcgtgtctg tttcgctcag gatgttcact
      841 ccagatttct gattccggga tgcttttccc gtcataactg atccgttcgc catcaaacgg
      901 gtaataccgt gcggaatcag tatctaaaat aaacaccttc agattcgttg aacgacgcgc
      961 aatatgctgc cagagagctt tacctccttc atattgctcg ttatcactta atagtacaac
     1021 gcccgctttt gtgactatcg tttcataaac aaacgttgca agaccaacat cacgtacagc
     1081 gccatcgaca acaatccctt caatctgagc aacatcctgt tcttcccaga atttctggac
     1141 aaacacgcct tcaagccggg gacgacgctg aagctccaga gtggcaacaa ccttatggcg
     1201 aacatcacca tcaataacag aaggtgcagc ttcgattacc gcagcaaacg gagtctttgg
     1261 atcccagatg atctggtaaa gcacgccctg aatagttact ccatccagta tcgaaaaacg
     1321 acgagctaca gtagcagggg agtaagacat tggaattaca gccatttttg aatcccctct
     1381 gagtaatacc ggcacgaaaa tttacctcaa taattatagt aaacaataca agaaccaagt
     1441 gcatcaacaa tacccatcaa ttgtacacaa ttcgtacagc gaccaggaac tccctcacct
     1501 tctgccaaac agattgacaa cattattgcc tgtttgctgt tgatagttat taacccggtc
     1561 ctcccagatc tcaagtgctc tgcgtttttc tggcaaataa tcgtatcgat cataatgttt
     1621 tgaactgaca tcgttcaacg catgattttg aatacgatcg cggatttcct tactgatccc
     1681 agcttctccc attaacgttt tacaggtccg acgtaagtca cgtgctgtaa aaattttaaa
     1741 gtcaggatta aaagcccgga aatacatgat agcgcgagcc agactgtccg tacgaaccgg
     1801 acgcttacca ttagtcgaaa gtggaaaaat gtaagggctg gatttttctt ttgttaactc
     1861 ctgcacacag gataattcct gtaatgctga ttcagtcaac ggtatcaaat gctctcgttt
     1921 atttttagag acatcagcag tcaccagcag cgttttttgt tgaaaatcaa catcacacca
     1981 ctgactggcg atcatttcga agggacgttg ccctccggta tagatacaga atcggatcaa
     2041 atgttgcatc aataatccaa cgttggttgc ttcagcaaaa tgttccatta gaaaacgcaa
     2101 ctcttcaagt gtcagccagg tatcaccgac tttttcagca gatgactgtt tcggtatcac
     2161 tgacaccgga tttgcctcca gcccaaaagt aatccctgca cttgtgttca tcggatcgtt
     2221 atctgctttc agtccatagt taaatgcagc catcaggtaa gaacggatcc ggttagagtg
     2281 aaccacagca ccacgctgaa tgatcccggc caggatatgt ttaatctgtc cggaagtcac
     2341 atctttagct ttagtttcac gggggatcac tgtatagcat tcattttcaa ggcgtttcag
     2401 aacatcctgc catgtcctct tattatcaat tttcattttg ttaacataac catgaatcaa
     2461 tgcttcaaat gagccctgtg attgatggag ttgtatccgc tgctgttctg caaactgttg
     2521 tcgttccagt tctgcctggg gatcctttcc ttcaatgagc caggcaccat acttttttgc
     2581 cagttcattc gctgtggcca gtttcatctc aggccatata cccaattgaa tgaatctttc
     2641 ttttttccct ttttccacgt aatagcggaa atagaaaatc ttgctgccag aagactgaac
     2701 tttgacacca agtcgtccag tgccacgttg agagctgttg ctccagacat agaacgatgt
     2761 cttttttgct ttcaatgctc ggatggctgt ttcagtaaga ttgacggcca tagaacctct
     2821 tttctaaacc agtaacaatc agaataactc gtgattttgg gtcaggtaat gggtcaggta
     2881 acgatcgtac aatgaagaaa aaacggaacc aagcggagac agagaaatta cataattcat
     2941 tgattataaa caactaataa agcaatcgcg aacaaacaaa aacaatatag aacga
//
LOCUS       DAZFAH010000324         2991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFAH010000324 DAZFAH010000000
VERSION     DAZFAH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.326"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHJWB_004763"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHJWB_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4316232.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHJWB_004764"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHJWB_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4316233.1"
                     /translation="MNISSLRKAFIFMGAVAALLLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHJWB_004765"
     CDS             902..1549
                     /locus_tag="ACHJWB_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4316234.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="ACHJWB_004766"
     CDS             complement(1591..2121)
                     /locus_tag="ACHJWB_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4316235.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHJWB_004767"
     CDS             complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHJWB_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4316236.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          864 a          592 c          662 g          873 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtt actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     1381 tgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggc actttcccca ggcagcgttt aatattaaag ggggagaaaa taatcacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt aattaacagg acagggctta taataataat agttagctta ctgtatatta
     2221 attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg gtggtgttat
     2281 ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc accccagccc
     2341 ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt attaaccact
     2401 tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt atacgggact
     2461 ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac aaagtgatta
     2521 cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc ctgatagttg
     2581 gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc taacaacgta
     2641 tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca
     2701 tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg ttggttattg
     2761 cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag gctgaggatg
     2821 agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa aaccataata
     2881 gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt aacgtaaacc
     2941 tgtctgatgt ttatatccct tttgtttagt tcaattccat tgattttggg g
//
LOCUS       DAZFAH010000325         2985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFAH010000325 DAZFAH010000000
VERSION     DAZFAH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.327"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..74
                     /locus_tag="ACHJWB_004768"
                     /pseudo
     CDS             1..74
                     /locus_tag="ACHJWB_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(179..249)
                     /locus_tag="ACHJWB_004769"
     tRNA            complement(179..249)
                     /locus_tag="ACHJWB_004769"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Met,seq:cat)
     gene            complement(413..970)
                     /locus_tag="ACHJWB_004770"
                     /pseudo
     CDS             complement(413..970)
                     /locus_tag="ACHJWB_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(1241..1795)
                     /locus_tag="ACHJWB_004771"
     CDS             complement(1241..1795)
                     /locus_tag="ACHJWB_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4316237.1"
                     /translation="MIYPTNTGKSGERLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLVHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDSHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(1792..2082)
                     /locus_tag="ACHJWB_004772"
     CDS             complement(1792..2082)
                     /locus_tag="ACHJWB_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4316238.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(2082..2681)
                     /locus_tag="ACHJWB_004773"
     CDS             complement(2082..2681)
                     /locus_tag="ACHJWB_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO4316239.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSISFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(2753..2965)
                     /locus_tag="ACHJWB_004774"
     CDS             complement(2753..2965)
                     /locus_tag="ACHJWB_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316240.1"
                     /translation="MEKIKKLFSCKYAVIRRDDLSVIVEMDYFPETPKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          754 a          780 c          668 g          783 t
ORIGIN      
        1 cagcacctga atccgcgatt accccatata cctggttgct gattgcccct ccgcacaggg
       61 ggattcacca tgccagtttc ttttaacaaa ctccccgcaa accagacaac agtcaaccgc
      121 ctgaattgtg agacatttaa aaaaaaggcc cgcaaaagcg agccagggaa aataagtgtg
      181 gcgcgttgta ctggattcga accagcgacc tggcgattat gcgtcgctcg ctctcaccac
      241 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaattccgc aataaaaaac
      301 ccgctcaatg gcgggttctg gtaaagttca tgcgcttggt tcgcctcgcg atacagcttt
      361 gcgaagcgta ccggaattga agcagtttat gtgcaaaaat gcaagaactt ttttaaagct
      421 gcatcaacct ttccaccagt ttatctctgc gaacaacaaa ccaaccattg gctctctcca
      481 gttccagcca tgactcaagg gaaataacaa tatcatcatc ccgtaactga attgtggaaa
      541 cagtgacacc acctcactga taacagagaa ctcgcgtgtc gtaacttttc tggcatgcaa
      601 ctggcgctga cggatccttt tgactgaaat agcagtcttc cagtttttcg aacacatccc
      661 acgcctgatc ggtttcgagc atttttgcgt ggcgggctgc tccgcgttct gtccagagga
      721 taagggagcg ggctttggga gagatgggat tttgtgagta gtttaaagct acccgcaatt
      781 ctttaaggtc attaccaaca actttgaaaa agtgtttccc ttcaacgaag cgtactttgt
      841 tctcatgatg attctggcga atacgcaccg gctcagtgcc gtaaagctgc gccaaaagtt
      901 cggtggtaat aacaggaatc cggttatggg tgatcgggga gagagtttca acagaaattt
      961 gagttgtcat aatgacgccc tctggtggtt tctaaactat caccaccatc aggttccaat
     1021 catcgggtgg tgagacgcac agggttggaa ctaccgggaa accgaccggc gagcttttca
     1081 gctcccccat gcgccccacc ataattcaga tgtgcgcgtg catacgacaa taaaaaacac
     1141 gctcgcggcg tgtgtctgtc gcggtctcta tccggggttc caatcccgac gccagatttt
     1201 gctggcgcgt gaggaatata gccccggata acagattgag tcaacagacg gtttttagat
     1261 ccccggaaga gaatgcatca cgcatcggta gatagagcat atattcagca agatttagcc
     1321 atacatctac ccgactgcag cacgtccgaa aacaccactc agggtgtgaa tcattcagca
     1381 attcagccat ttttcgctta gtcatccccc tcccttcata tcgttgccgg aggatacaaa
     1441 tcaatcctgg gtgttctgcc agcacttcac taatcacccg atcaatgcat aacgcctctg
     1501 catcagtaca atgcaccagc cagctttttt gcttgccgtt gatcatatcc cgcaaaaaag
     1561 cctcaagttc aggtttgtcc aaacctgctt ttttcatcct ccggagcgcc tcgttaattg
     1621 ccgtttttgt cagcttttta gaggtcagca actggttgaa catatttccc gtcttaccgc
     1681 cgccaatata cgaccagcgc ccccacatgc gcagttttcc ctgaatccag acactttcca
     1741 gcgtggtgag acgaaggcgt tccccgcttt tgcctgtatt tgttgggtaa atcataaata
     1801 accttccttt ctccagattt cttgtgtgcg aaaaacacct tctgcatgca tcaggcgcaa
     1861 ttcttctttg gtgtaatcgc tggtttttac ccgcccgtcg attaaatcgt ggcatgagct
     1921 acaggcaatt gccgcctgca tatcgtgtgg ttttgtcgct gttccacacg tccccgccag
     1981 cctgtaatgc gccagcacgg acgtttcggg attgtgattg cagtagccag gaattctgac
     2041 ggtgcacatc tgcccccgcg ccgctttacg taaatccacc attacgcaaa ctccagtagc
     2101 tgcgcggcca cattttcgac ttgttccgga gaggaaaatt tacggaacag gatccagttc
     2161 cacagtacat tcagcacaga tttataaacc tgctgaaact cggtttcgtc catattcgca
     2221 aacgagatgg atttcgccct gcgcccacgg ctaccgtccg gataaatatg ctcggtgtaa
     2281 aatccggcct gaatggttac ccactcgcgg aaagcctcaa acgactttag caatgctgta
     2341 tctcgggttc tgcgtgtcgc aactgtattc agatattgct ctgcggcttc gctcagagct
     2401 ggcgtatgtt cccggcctac tgattcacac agataatcaa cgaaaccgga caccagtttt
     2461 cgttcgcgag gagtgatcgc cccaccgacc ggagtccagt aatcgaatcc gagttgcaga
     2521 agtttgaaaa aacgcttatg gaatgcgtag ttacgaacgc gcttaaaatc agcgtgtatc
     2581 cactcgccta ttttgatttg atgcagaaaa tcacaactct ccggcgtcgc cgggagaagt
     2641 aatccggaag aggtttgttt gaccagttgt atatgcgcca tcgtagttct ccgctggcgc
     2701 agtagaatgg gtgttcagcc cgttatgtag tataccagaa ttaatgccaa tactaacagg
     2761 atgctctgac tcgcaattca tccagcagtt tatcatttcc cataatgtca cttaccctca
     2821 tcggtaaaaa aattgccttt cgaccattac gatacatcat tgattttggg gtttcaggga
     2881 agtaatccat ttcgactata actgacaggt catcacgacg tatgactgcg tatttgcaac
     2941 taaatagttt ctttattttt tccacgatgc ctccgagttt ataag
//
LOCUS       DAZFAH010000326         2978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFAH010000326 DAZFAH010000000
VERSION     DAZFAH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.328"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..>2978
                     /locus_tag="ACHJWB_004775"
     CDS             2..>2978
                     /locus_tag="ACHJWB_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO4316241.1"
                     /translation="MSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLTIGS
                     DWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGV
                     PPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLY
                     YDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTLITFCNEE
                     IIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALANIE
                     KYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESL
                     IRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINK
                     FNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICPYALMVYGVG
                     DGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGELYFEAHQ
                     GCFTSESATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDEIWKAMLTLQFHDILP
                     GSCISRVYHETEKEYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNE
                     WININNNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSESGDLISLYD
                     KRYGKEYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTT
                     MTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQ
                     FGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLELTLL
                     RSQHKPGEVVKADNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIMNHPL
                     QIVPVKPHPGELPPSLSFCAIDTNSVIIETIKLAEDGDGIIVRLYESYGLEISAMLSW
                     VCNYHYVQYVDLQENKLDDS"
BASE COUNT          937 a          585 c          582 g          874 t
ORIGIN      
        1 actgtcgaat aaaataggtc agcgtgttta tactgttgtc tctgaaatgt cgattgaagc
       61 atggataaca aaagaaccgc agccttttgt ttatcgccgt agcggcgctt accagaaact
      121 caccattggc tcagactggg gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa
      181 acgtccagca gataaattta gacacaaact cgtcgctctc gttgatatcg gtggcgaagg
      241 tcttattgtg gataagaatg gttcacccat ctgcggcatc acgaataaag catcatctta
      301 tggcgtaccg ccagataaac caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa
      361 tgacgaggtc gagttttgga ttgatgctgc gtgcaacgat ttatttggct atgtcactaa
      421 tggagggatt attactgatg ttcatatcgc gacatgtaat caactactga aatcccttta
      481 ttatgatgtt gaagtgcttt tcgactggat taatgatggt cagaagtttg aatccataca
      541 tcctaaaggt attatcccag aaaaaattat caccaaaaga agcgagcgta ctgacgaaat
      601 cataagaatt ctcgaatata ttgataatac gttaattacc ttttgcaatg aagaaataat
      661 taaatgccaa attgcaatcc aatccataat tagtaaaaac aataatcctt cagagttcag
      721 gattatggct actggtcatg cccatttaga tatagcgtgg atgtggcctt taagagaagg
      781 acgcagaaaa gcaataagaa catttgccac cgccctggca aacattgaaa aatatcctga
      841 ttacattttt ggcgctagtc agtaccaact ttttcactgg ataaaaaaag attatcccta
      901 tttcttcgaa aagttaaaaa aacaaatcgc cgcaggccgc tttgagttac aaggttgctt
      961 ttgggtcgaa tgcgatttaa atctggtaag tggtgaatcg ctgataaggc aaatcatgca
     1021 tgggacacga ttcacactgc agaatttttc aaagaagata aattatgtct ggcaaccgga
     1081 tgtctttgga ttccccgcca cgcttccaca aattctcaaa aaatcaggca ttaactatat
     1141 tgcatcgcaa aagctctcac aaaacaagat taataaattt aataattacc ttttccgctg
     1201 gcaaggcctg gatggctcag aaattctgat gcataacttt cctgaagata cgtatgatag
     1261 ccgagcccgc gctcgctctt tagaatatat tgaacaaaac tataatgaaa aagaaatctg
     1321 tccctatgcg cttatggtgt atggtgtcgg tgatggcggc gctggccccg gtgaggaaca
     1381 tatagaacga ttaacacgca tacgcaatat tgatggctta cctcatgttg atttcagccg
     1441 tgttgataag ttcttcactc acgccgacgc ctttcgcgaa tccttgccca ttatttcggg
     1501 tgaactttac ttcgaggctc atcaagggtg ctttacatca gagtcagcaa ctaaagccca
     1561 taatcgaatt atggaaaata aactccatga tgctgaattt tttataacta ttactaataa
     1621 tatgacgtca attcttcgca gtgagtttga tgaaatatgg aaagcgatgc tgaccttgca
     1681 attccatgac atccttcccg gctcatgtat ttcaagagtt tatcatgaaa ctgaaaagga
     1741 atatcttaaa ctagaagcaa aaaccgagaa aattatttct gatgcgcagt ctactttatt
     1801 aagtaaaatt gacacttcat cttataaaga tccgcacatt ttatttaata ccacctgttt
     1861 tgctcgtaac gaatggatca atataaacaa caactggctc aaagcacgag taaactctta
     1921 tggttatgct gtgattgatc caaagaataa gatcgtaaat ggtctgaagg cagaatctcg
     1981 aagtattgaa aataattaca ttaaacttct tttttcagaa agcggtgatt taatttcgct
     2041 atatgataaa agatatggta aagaatacat tactgaaaat atgcacagtg agatccgtgc
     2101 ataccacgaa gatgcaggtt ttttcgcggc ctgggatttt gcttcaaatt atcgtgatgg
     2161 ggaatcatat gttttactcg ctgaaaaaat gacaactgtg atcagcgggc ctaaaactac
     2221 gatgacgctg atttatcact acaattcctc ttatcttcgc ttcgcattta ccctgacaca
     2281 ggacagtcca cgcgttgatg tccaaacttt tattgattgg cacgaaccta atgtttctct
     2341 caaagtaaaa ttcccggtat cagtacaaac atctcttgca caatgtcaaa ttcagttcgg
     2401 tgtaattgac agaccaacgc atagcgatga ttcgttcgct tttgccaaag atgaaatacc
     2461 agcacatcac tgggtcgacc tttctgatca agaaactggc attgctttaa tagcaaaaaa
     2521 caaatatggc tatcgtgtca aagaccagac gctagagtta actttgttgc gttcgcagca
     2581 caaacctggg gaagttgtta aggcagataa ttatgataat ttcctgatta ataattttgc
     2641 agatattggt aaacaaaaat tcacattctc cttatttcct catcatggag attaccgtgt
     2701 gggtggtgtc gttaaccagg cctatataat gaatcatccc ttacagatcg tccctgtcaa
     2761 accacacccg ggtgaactac ccccttcact atcattttgt gctatcgata ctaacagcgt
     2821 tatcatcgaa acaataaaat tagctgaaga cggcgacggt ataatcgtaa gattatatga
     2881 gagttatgga ttagaaattt cagccatgct ttcgtgggta tgtaattatc actacgttca
     2941 atatgtcgat ctccaagaaa ataaattaga tgacagtt
//
LOCUS       DAZFAH010000327         2932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFAH010000327 DAZFAH010000000
VERSION     DAZFAH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.329"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..1655)
                     /gene="cadC"
                     /locus_tag="ACHJWB_004776"
     CDS             complement(117..1655)
                     /gene="cadC"
                     /locus_tag="ACHJWB_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4316242.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1915..2029)
                     /locus_tag="ACHJWB_004777"
                     /pseudo
     CDS             complement(1915..2029)
                     /locus_tag="ACHJWB_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2404..>2932)
                     /locus_tag="ACHJWB_004778"
     CDS             complement(2404..>2932)
                     /locus_tag="ACHJWB_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4316243.1"
                     /translation="NTDVICSTSVRSRSLEYDNFPEISEANILSTFEQLHQNKDEVFE
                     RGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLH
                     LLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKGSAHITFKRLELIDR
                     INDIIARYFPSVLSA"
BASE COUNT          830 a          600 c          621 g          881 t
ORIGIN      
        1 aaatttatgt aataaaaatt atgcggcaaa taaattgccg caacatatta taccaacaga
       61 aacatacaaa aactcaacaa caaatattcc cgagcataaa tcaacccggg agttacttat
      121 tctgaagcga gaaatttgtc gagataaggt acaacataag gaacagaagt ctggaatata
      181 ccattttcaa tccagtaaag ggtgtttgcc cctgggcgta aattaaaggc ggtgagatat
      241 gcatcagccg cttcccggtt catccccttc atttcataaa ccttgccaag caacacataa
      301 tttagccagg acatttcaag atcaatgcca gtatttatcg cctggtaaga ctcatctgtt
      361 ttacctttta ccagagcact gaccgctttt atttgatata taatggacag gttattcaat
      421 tccggcagtg taacaatgtt gtctatttct gtgttcagtg ctgctaattg tttttcatct
      481 aaaggatgtt gagaatggcg cacgatatca actaatgctt tttctgctct cgcgtaggta
      541 aattctgggg atgattgaac aatctcacct aataattcac tggcacgatt caatgattta
      601 tcatcgccat gcagtaaata atcatgtgcc tgataaaaat tagttaataa cgcaccacga
      661 tgaggcaata tttgctggag cgtctcctgc attcgttgtg gccacggttg gtttaacgct
      721 tttgataaac tctccagtaa atcattttga atcgccagct gattaccgtt agtgatgaca
      781 taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttat cgacataaag
      841 cattgttgag cacggtattg gcgctgatta acaaacgcaa tagataatgt tttaccggaa
      901 ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt ggagaaggtg
      961 ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg atacggggaa
     1021 ctccagctgt tacaactttt atttaccata ttaatgtcaa tatcgcgtgg attgagcaaa
     1081 atacgcgatt tgctcatagg aagacgtgta tcaagagttg aaaacgctac cagtgctaca
     1141 cagataccta acgacaacag gaaaaaaaac catacccaaa aggtagtgaa tcgtttgctt
     1201 ttaactgggg attgttcagg tggcgttgcg gtgttttgaa tgttaagact gtgggaggga
     1261 gaatctgtgg caggaaccgc ctctggtata gggggaggcg aagatagcat tatttcctct
     1321 ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg ctttggtaca
     1381 gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg tagttctgag
     1441 atactctgcg tcacaacatg attggtgaca atacttctct tccagacatt atcgataagt
     1501 tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag atcgattaat
     1561 ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga cggagtaaca
     1621 agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc gaaaagggaa
     1681 tgatgcaatg ataattgcca caacctattt ttaccatcta tagatgggtt tatttacata
     1741 ttattggtga atgcaagacg ttatttttac cagccataaa cttcttgatt acatagtatt
     1801 acgaaaggat tttactgaga accagaagta atattcctta ccatcaaaat tcatcatctt
     1861 tgccaaagaa aaatgttcag aaaataatcc atggaaaatt gtccggagcg cttactattt
     1921 taatggattg ttagtctttg catgagcaag cggactgata catttctctt tgttctcatt
     1981 cagaaaatct catcagtcgg cgttcagacc ggtgttgtgc tttatggatg cgttacgctc
     2041 ctgatgacgt catttgacgt tcaacaacat cacggggccg cacgacattt cacgtcagtt
     2101 agtgctatcg ctcaggaaca aattttcccg aattgggata tgcccgcaaa ttgctggtga
     2161 tgtggggaga atctggttga gttcggtaga attgatttgg aggcagaacg cttaaattgt
     2221 ggcgtccaga aacgaaaaac ggacctccgt ggaggtccgt tgatatgaat ttggtgccgg
     2281 actcggaatc gaagaatgct tctatcatgt tgatattagt ttattttgtt tggtttggtg
     2341 aaatatgtac cctcttttgt acccgcagtc ccttttgata tcgaaaggga atcaaactcg
     2401 gggtcaggct gagagcacag aaggaaagta cctggctatt atatcgttaa ttctgtcaat
     2461 cagttccagc cttttgaaag tgatgtgtgc agatcccttc ttaaagtatt taatgatgaa
     2521 catctcatct tcatagcgtc ctttaccctg aacggactgt atgtggtcat ccagattaat
     2581 ggtgatattc cctcggttgt cggggatcgg tttaccgctg agcagatgca acattctttc
     2641 caggtcagcc aggcgatctg cctgcattcc actgataaga tgaaatcccc actggtccca
     2701 tctcaccaga ttgttgacga taattttact gccaaattta cagggtgagt tggttttgta
     2761 atcccagctc agccctttga agacgttgat cactccccgt tcaaacactt catctttgtt
     2821 ctggtgcagt tgttcaaatg tgctcagaat gttcgcttcg ctgatttccg gaaaattatc
     2881 atattccagt gacctggacc tgactgaggt ggagcagata acgtccgtat tc
//
LOCUS       DAZFAH010000328         2874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFAH010000328 DAZFAH010000000
VERSION     DAZFAH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.330"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(557..730)
                     /locus_tag="ACHJWB_004779"
     CDS             complement(557..730)
                     /locus_tag="ACHJWB_004779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316244.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            908..1111
                     /locus_tag="ACHJWB_004780"
     CDS             908..1111
                     /locus_tag="ACHJWB_004780"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO4316245.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(1043..1507)
                     /locus_tag="ACHJWB_004781"
     CDS             complement(1043..1507)
                     /locus_tag="ACHJWB_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316246.1"
                     /translation="MPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGTFFS
                     LVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRTVTE
                     VNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1436..2245
                     /locus_tag="ACHJWB_004782"
     CDS             1436..2245
                     /locus_tag="ACHJWB_004782"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO4316247.1"
                     /translation="MAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            2410..2818
                     /locus_tag="ACHJWB_004783"
                     /pseudo
     CDS             2410..2818
                     /locus_tag="ACHJWB_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          776 a          708 c          661 g          729 t
ORIGIN      
        1 gagttttttt catttaaccc tccttcatta ataaatgatt tctgaaaatc aaaaaggacc
       61 acacatctca aaaacattat ttgaaatcat catcaaaaaa ctataaatat atcacctgac
      121 gatttgaaca cactgacaaa aaaccaagca tggtaataaa ccgaactccc cacaaacttc
      181 tgttgcctca gatactcccc ccctttcctc aggcacaaat gttgttccat aacgaaccag
      241 ccctgttcct ttaaactctg catcaatggc ataatgtata ctcaccagac tgagtttgct
      301 cctgcatcat tatttcgata cgttaatgca ttgttcattg catccggcat tccccatcga
      361 atatttactt tgttatatca gtcagcccat aaccaggtaa atttttaagg cttactgact
      421 gaatattcaa aatgtgttac atgttatcaa acctttaaag aataacctgc tcttcttcat
      481 tggccggatt tagctcaatc ttgtcagcaa aactaatata aagccgttca taaagagaag
      541 gatgtagctt cgttatttat cttttacatg taacagtgcc aggggatatt ccgcacatct
      601 ctgtggctat catccccacc acgtctccag tggcagcacc tgcggcactg gcacgggcgt
      661 tatttccctg taccaccgcc agtgcagcat tcaccacggc atgggctgcc acatttgcag
      721 tcttattcac cacacctgta acctgtctct tatacacaaa tccccacccg gggatttgtg
      781 atgtctgtca gtctgcttta gggggactct ttccatccgg tagtttcctt cctccccggc
      841 agcgattttt tgaacttttt cttcacccgt tgatcttctc ttatatttca ttttcttcgg
      901 gtctgttatg gtactttctg attgttattc actggctaac gagcagtctg gacatgcccg
      961 tctcggtgat ccgcgtcgta cacgacgcct tgtgtcactg acctcttctc tcgctcagca
     1021 tgcaggtctt tctattgtga aatcatccca cttcactgct caggttgaag gtgcgtaccg
     1081 cctgatacgt aacccgtcgg tgtccccgta agccattgcc gaagccggat ttactgccac
     1141 tgcaagggca tgtgaggcac atccgctgct cctcgcgctg gaagatacca ccaccattaa
     1201 cttcggtcac agtacggcat ttgatgacct ggggaatacc accactaacc cgaagacgca
     1261 cgggctgctg gctcactctg tcctgatgta cgcccccgac agcgccctgc cagtcgatct
     1321 tatcgaacag cagtgctgga gtcgtgccac ggacacctac ggtgttaagc accagcgaaa
     1381 agaacgtccc tgtgaagaaa aagaaagtta ccgctggcag caggcttcgg agcgcatggc
     1441 cgaacggctg ggggagatac agaaacaggt aatcactgtc tgtgaccggg aagcggatat
     1501 ctggcattac ctgcattata aagtcagccg cgggaagcca cacgctgaat gtgatgcaaa
     1561 aaggcgggcg ggcagcccgc caggcccgga tgctcatcag ttacagcgaa gtcagcataa
     1621 aaaatcccga caacagcggc caggcgctct cgctcacgta tgtctgttgc ggggagcagg
     1681 agaggacgga gcctgctggt atccgctgac atcagaaaaa gtggcgagtg ccgccgatgc
     1741 gcgacgtatc gtcagccatt acgagcaacg ctggctgata gaggaatacc acaaagcgtg
     1801 gaaaagtggt ggtacatgtg tggaatcgct gcgaatgcag acccgggata accttgagcg
     1861 catggtggtt atcgcggtgc gggtgctggg cctgcggcag ggaggtatca gtgaagaaac
     1921 acaaaatgac agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa
     1981 gctggaagga aagcagcttc cggcgcaaac accgaccctg aaatgggcct gtttgaaact
     2041 ggctaaactg gggcgatggc atgacagcaa acgcacaagc agccccggtt gggtagttat
     2101 gtgggatggc tggttcagac atcaggatat ggctgagggt tacctggtga tgaagtctct
     2161 tgatcaggag atctgctcaa gagacaggtc aaagatgggg gacaacgtct ggtgctgtat
     2221 aacagcacag gcctcacact cttgacgccc gtggaatata ttagagaaca aaaatttcaa
     2281 tatgtggtgg acgcatacag aggattgaca gcattcccat actaattgtt aagattcaaa
     2341 ttgaagtata tctatctgtc attttgcaaa tgatatgcgg tagctttacc taactatcat
     2401 gaacatttcg aaaaatcatc cacaaagccc cgaagaataa agcactatcg cccgataaag
     2461 acctgtaagc tcagtggcat ggatactatt gagctcagaa tggcgagctg taccgttatt
     2521 tcattaccat gctcgacgaa cacagcaact actctctggc cgtactatcg cttaaaagtg
     2581 atatcatcag gcatttcttc tgctgtgtag cccagttgtt ccttgttggt atcaaccaga
     2641 tcatcactga tgatgggcaa agagttctcg ggaaaattcg acaaaacgct acaggaagcc
     2701 accgtcgggt tcgtatggac ttatctctat acgccgaaaa ttaaatgcca taagtgagca
     2761 ttttaaccga acactgaggg aacaatttat tgagtttaat gaagaattgc tctttgaata
     2821 gctgaatatg ttcaatctga aactggctga gtatctggta ctgtacaaca gtaa
//
LOCUS       DAZFAH010000329         2830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFAH010000329 DAZFAH010000000
VERSION     DAZFAH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.331"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1257)
                     /locus_tag="ACHJWB_004784"
     CDS             complement(<1..1257)
                     /locus_tag="ACHJWB_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4316248.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKYLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKI"
     gene            complement(1498..2544)
                     /locus_tag="ACHJWB_004785"
     CDS             complement(1498..2544)
                     /locus_tag="ACHJWB_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4316249.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(2648..>2830)
                     /locus_tag="ACHJWB_004786"
     CDS             complement(2648..>2830)
                     /locus_tag="ACHJWB_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316250.1"
                     /translation="SCCCSSDHYSIMIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQ
                     NMVGGIVRRLVIVGMT"
BASE COUNT          773 a          704 c          573 g          780 t
ORIGIN      
        1 aattttaaaa tcagattcct ttaatacttc ctcatattca ttcagagaaa cttgtttttc
       61 ccggatgaat ttttcataat aagaaagctg aagagaagta aatagtgcca gccttctctc
      121 cggaatcacc aatggtttga ctttcagtat cctgaagttt aacaacaaat caattctgtt
      181 tctgaatcca atccgcctgt cagaaaggta atgaatataa gccatcaggt cagttgtttt
      241 tcgtgaggaa agtcggtatt tgtgagaaaa aagctcctcc ggtcgaagat tctgactctg
      301 ccattgctgc aggtatttat actcaatatt cagctcatta atttctgctt ttaacgaagc
      361 gacctggtta atggcattca ggtatttttt cacttgttga agtacatcat caatcgcatt
      421 tgcagacggt gagtcaggta agacttcctc agaacgcgaa atcgacgttg aaaaaaactg
      481 ttgcctgttc tccgtactcc ccagtcgtgc caccacatat cccagttgct gtttgtccat
      541 cttttcataa acattactga cagctgagtt attattggac aaaattgcca ctgttttatt
      601 ctgaattaat atattagcaa caatattgag tatagtctgt gttttacctg ttccaggtgg
      661 tccttcaata actgaaacct gtgaggaaaa tgcccgttcg acagccagca actgactttc
      721 attaagacca aacggaaaaa tcaaacccca tggcgaatca cgttttttac tctgtcctga
      781 acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct gcctaaggat
      841 attctcatcg atatttctgt tattaccgcg ggcataaaaa atacgttcat tagccaccct
      901 acacaagtaa ttaaacacgg catgttctgt aaatgctgaa cactgttgaa agtcaagacc
      961 ggtcgttttc atgatgtagg gtcgttcacc ctcgcgataa taaacagcag catatttatc
     1021 accatatata ataaccagtt cagcccggga tgttacagcg ttccctttcc tttcgaggaa
     1081 aacatttcgc aaatttacag ttggcgttat ctgacataca gaaagcggac gagtatattt
     1141 tctcccgctg cgaaaatgac aggtcagcat aacctctctg tcgtcatttc cagcccacag
     1201 cgtccagtcg gtaattttat gagtcttatt ttcaccgtca acacaaatcg tgaccatatt
     1261 cttactcaga aattatcatt taacttacgg aaaattgtta aggcactccc gatattagct
     1321 ccggaggatt ttccacaact ccgggactgt taacatattt ctgcctgcca tatatacatt
     1381 gaatttagtc ataacagtca ataccagaat tacaggaatg cgatgccgat acgctggtta
     1441 ttgaatactg gtagcccctg agcactaatt catcacgata ctctgtattt actgttttca
     1501 ctgcttttta caaccattcc cccaggtaaa atgctgctcc tccggtgatg gctgtgtcag
     1561 aaactgccat cccggaatag ccacgctgac cgccagcaat gccgcacaga caccagccac
     1621 aaacggtttc agaaacgtgg gcggggattt ctgaagggag ggtgactgct ccgcctgcac
     1681 tttccccttg cgattatcaa ggctcagtgc atccagacgc accgcgccat ttgccgcaag
     1741 cagcccttcc cggttatcca cgcttcccgg cagactcagc gaaaaatccc ctgttcccgt
     1801 ctgaatcaga ctgcctttac gatttgacag gctttgtgcc gatatctgca gtgtgtcggc
     1861 atttatcgta ccgccattac tgctgaactg cccggcagtc cgggcggaaa gcgtatcggc
     1921 actcagttgc gcattctgtg tagacagatt tccactggtt gcattcagct cagtatgttg
     1981 tccctgtatc cggctgttac ctaaatccag cccctgctca atgtcccgta aatccagcag
     2041 agaaccttcg tgtgccggga atgaggaata caactgaata tagtcatcac tgtccggtgt
     2101 cagacgcaca tgtcgcacaa caccgggaat aataaccagc cggaggatac cgctttttaa
     2161 atcctgcgaa ggtgccagaa cacgggtggt gacataacca tgatcgacca gacggttctg
     2221 caatgtactc atcagcagat taattccttt cgcccccagg caatgcccga ccgccccgtt
     2281 ggcgattttt tgtaaaggca gccagtgtgg taacgcatca gcccccttca gttccgtctg
     2341 tttaatctga aaacagggcg tttcaacagg aaaattaatc ttatgggcaa aagagccagg
     2401 tgcagaaagg cgaacatcag gggctgaagg cgtcagttgt tgctcaaggg cttcctgacg
     2461 ctgctgctga atgataaatg cttcatctcc gctacgccgg acatccgctg cctgaagaga
     2521 taaaggcgat aataaagcag acatcagtgt cagtgacagc aaggacggac agcgccggaa
     2581 taagctttgt tcctcagtcc tctcgttcct gcaacataat tttccagtca aacgaaaaac
     2641 cttttattca tgtcatacca acaatcacca gcctcctgac gatacctccc accatgtttt
     2701 gcgatggcac gctaccgccc ttccggtatt tacccgcatc aaccggtacc agaatgctga
     2761 gtaacaaacc agctagaatt gacttaattt tgatcattat agaataatga tctgaactac
     2821 agcaacatga
//
LOCUS       DAZFAH010000330         2810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFAH010000330 DAZFAH010000000
VERSION     DAZFAH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.332"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(69..145)
                     /locus_tag="ACHJWB_004787"
     tRNA            complement(69..145)
                     /locus_tag="ACHJWB_004787"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Asp,seq:gtc)
     gene            complement(278..1009)
                     /gene="dnaQ"
                     /locus_tag="ACHJWB_004788"
     CDS             complement(278..1009)
                     /gene="dnaQ"
                     /locus_tag="ACHJWB_004788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4316251.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1074..1541
                     /gene="rnhA"
                     /locus_tag="ACHJWB_004789"
     CDS             1074..1541
                     /gene="rnhA"
                     /locus_tag="ACHJWB_004789"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4316252.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1538..2260)
                     /locus_tag="ACHJWB_004790"
     CDS             complement(1538..2260)
                     /locus_tag="ACHJWB_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4316253.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2294..>2810
                     /gene="gloB"
                     /locus_tag="ACHJWB_004791"
     CDS             2294..>2810
                     /gene="gloB"
                     /locus_tag="ACHJWB_004791"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4316254.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNM"
BASE COUNT          743 a          695 c          667 g          705 t
ORIGIN      
        1 ttcaaataca aaaaaaccgc aaaattaaaa atcttgcggc tctctgaact cattttcatg
       61 agtgaatttg gcggaacgga cgggactcga acccgcgccc ccctgcgtga caggcaggta
      121 ttctaaccga ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac
      181 ggattgcctc acccttcgtc aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat
      241 cgcccaagcc gctattttta gcgcctttta caggtattta tgcccgccag aggcagcttc
      301 cgcccttctt ctccaccaga tcaagacggg cttcatgagc tgcaagctct tcatctgtcg
      361 caaaaacaac gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt gcttcacctt
      421 gttgctgctg tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg
      481 ccagataaac ttccgcaagg atctgtgcat cgagtaatgc cccgtgtagc gttcgtttac
      541 tgttatctat ttcgtagcgg gcacataacg catcgaggct gttgcgctta ccaggaaaca
      601 ttttcctcgc caccgcaagg ctatcggtga ccttacagaa ggtattggtc ttcggaatat
      661 cgcgcttaag caacgaaaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga
      721 tcaccaactc cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg
      781 gcttatcgag caaaaattca tcggcaatac catgtacgcc aaaggcttcc ggatccacca
      841 gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt
      901 caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac
      961 cggtggtttc ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt
     1021 atgtcagact tgtcgtttta cagttcgatt caattacagg aagtctacca gagatgctta
     1081 aacaggtaga aattttcacc gatggttcgt gtctgggtaa tcctggacct gggggttacg
     1141 gcgctatttt acgctatcgc ggacgcgaga aaacctttag cgcaggctac acccgcacca
     1201 ccaacaaccg tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt
     1261 gcgaagtcat tttgagtacc gacagccagt atgtccgcca aggtatcacc cagtggatcc
     1321 ataactggaa aaaacgtggc tggaaaacag cagacaaaaa accagtaaaa aatgtcgatc
     1381 tctggcaacg tcttgatgct gcattggggc agcatcaaat caaatgggaa tgggttaaag
     1441 gccatgccgg acacccggaa aacgaacgct gtgatgaact ggctcgtgct gcggcaatga
     1501 atcccacact ggaagatacg ggctaccaag tcgaagttta agcttgtggt ttacgacatt
     1561 gccgggtggc tccaaccgcc tggcgaattc gtggcttgtt tttactctgt ttcatcgggt
     1621 ttagcgttaa aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag
     1681 gaatatgcgc attcaatagt tttcctccgt gtttgttcca cgggagaacg tggaaacggc
     1741 tggcgtgcag cacttcaaaa ttcaacaaag agagccagtc cagttgccgc atcagagtaa
     1801 acatccggct gttatagggc gaggtttttc gcaataccgg cacaagtttg cgtaatccca
     1861 ttaaactaat gggattgaag ccactaatca ccagccagcc atcatcaatc aatacccgat
     1921 cggtttcacg caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccagtagac
     1981 aaacatcaac ggatttatcg gcaaaaggaa gatgaagtgg atccgcctgc acctggacgg
     2041 gcattccttg cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt
     2101 ctgcgcttaa attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt
     2161 tgagctggcg ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcgat
     2221 caggagccac gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac
     2281 taagaggtaa tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt
     2341 tttgaatgat gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccggtatt
     2401 aaacgccatt gccgccaata actggcaacc ggaggccata tttctcaccc accatcatca
     2461 cgatcacgtt ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg
     2521 tccacaagag acacaagata agggaacaac acaggtagtc aaagatggcg aaactgcctt
     2581 cgttttgggg catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg
     2641 ttacttcagc aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtag
     2701 gttgtttgaa gggacagcat cacaaatgta tcaatcactt aataagttaa gtgcgttacc
     2761 tgacgataca ttggtatgtt gtgctcatga atatacgtta tcaaatatga
//
LOCUS       DAZFAH010000331         2806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFAH010000331 DAZFAH010000000
VERSION     DAZFAH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.333"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..466
                     /gene="agaB"
                     /locus_tag="ACHJWB_004792"
     CDS             <1..466
                     /gene="agaB"
                     /locus_tag="ACHJWB_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4316255.1"
                     /translation="NILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQK
                     LMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDIN
                     VGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            505..1308
                     /gene="agaC"
                     /locus_tag="ACHJWB_004793"
     CDS             505..1308
                     /gene="agaC"
                     /locus_tag="ACHJWB_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4316256.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1298..2089
                     /gene="agaD"
                     /locus_tag="ACHJWB_004794"
     CDS             1298..2089
                     /gene="agaD"
                     /locus_tag="ACHJWB_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4316257.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2090..>2806
                     /locus_tag="ACHJWB_004795"
     CDS             2090..>2806
                     /locus_tag="ACHJWB_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO4316258.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFL"
BASE COUNT          677 a          666 c          715 g          748 t
ORIGIN      
        1 aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg gcgtgacctg
       61 gacatccacc atcggtgcga atctgctggt agtcgtggat gatgttgtcg ctaacgatga
      121 tattcaacag aaattaatgg gtattaccgc agaaacctac ggctttggca ttcgtttctt
      181 tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccgcatcaga agatcttcct
      241 gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggcattg acctgaaaga
      301 tatcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca gtaaagttta
      361 tgtcgatgac caggatctcg cggacttacg ttttattaaa caacgtggcg tgaatgtttt
      421 cattcaggac gtccctggcg atcaaaaaga acaaatccct gactaaatct aaaatcgcct
      481 taatattggt ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg attatccctg
      541 gcggcgttag ttttcgttct ggggattgat ttttggctgg aagccttatt tttattccgc
      601 ccgataatcg tttgtaccct aactggcgct attctcggtg atattcagac tggcttaatt
      661 accggtggtc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg
      721 cccaacccga ttatggcggg tctgatgacc accgtcattg catggtctac gggcgttgat
      781 gccaaaacag caattggtct tggcctgccg tttagtttgt taatgcagta cgtcattctg
      841 ttcttctatt ccgctttctc attatttatg accaaagccg ataaatgcgc gaaagaggcg
      901 gatacggcag cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc ttcagcgtat
      961 gcggtgattg ctttcctctg tacttacctg gcacaagggg cgatgcaggc gctggtgaaa
     1021 gcgatgcccg cctggttgac ccacggcttt gaagtggctg gcggtattct gcctgccgtt
     1081 ggttttggct tgctgctgcg cgtaatgttc aaagcgcaat atatccctta cctgatcgcc
     1141 ggtttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc cgtactgggc
     1201 gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag
     1261 cccgttgcca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa
     1321 agatatcacc cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta actacgaaag
     1381 gatgcaggcg ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga tttataagga
     1441 cgacaaaccg ggcttaagcg cggcgatgaa agataacctc gaatttatta atacccaccc
     1501 gaatctggtc ggattcctga tggggttatt aatttcgatg gaagaaaaag gagaaaaccg
     1561 cgacaccatt aaaggcctca aagtggcact gtttggccca atcgccggga ttggcgatgc
     1621 gattttctgg tttactttgt tgccgattat ggcgggaatt tgctcatcat ttgccagcca
     1681 gggaaacctg ctggggccga ttctattttt cgccgtttac ctgcttatct ttttcctgcg
     1741 cgtcggctgg acccacgtcg gttattcagt cggcgtgaag gcgatcgata aagtgcgaga
     1801 gaactcgcag atgattgctc gctcggcaac catcctcggg atcacggtaa tcggcgggct
     1861 gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca gtacccacag
     1921 cgtcgcgctg caacaggatt tcttcgataa agtcttcccg aacattttac cgatggccta
     1981 caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg
     2041 cgtgactttt gtgctctcta ttgtttgttc cgcattcggc attttgtaaa tggaacgagg
     2101 cactgcttct ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa cccttcagca
     2161 agttgaaaac tatacggcgt taagtgaacg agccagcgaa tatttattgg ctgtgatccg
     2221 tagcaaaccg gatgccgtga tttgcctggc gaccggagcc acgccattac tgacgtatca
     2281 ttatctggta gaaaaaatcc accagcagca ggttgatatc agccagctca ccttcgtgaa
     2341 gctcgacgaa tgggtggatc tgccattaac gatgcctggc acctgcgaaa ctttcctaca
     2401 acagcatatc gtgcagccgc tggggctgcg tgaagaccag ctcatcagct ttcgctccga
     2461 agagataaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca aaggcggtct
     2521 ggatttatgc gttctaggat tggggaaaaa cggtcatctt gggctgaacg aaccgggaga
     2581 aagcctacaa ccggcctgcc atatcagtca acttgatgcc agaacacagc aacatgagat
     2641 gttaaaaacc acgggtcgcc ccgtgactcg tgggatcacc ttaggcctga aggatattct
     2701 caatgcccgc gaggttttgt tattggtgac tggcgaagga aagcaggatg cgacagaacg
     2761 ttttctcacg gctaaagtct ccaccgctat cccggcttcg ttttta
//
LOCUS       DAZFAH010000332         2788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFAH010000332 DAZFAH010000000
VERSION     DAZFAH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.334"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..388
                     /locus_tag="ACHJWB_004796"
     CDS             92..388
                     /locus_tag="ACHJWB_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316259.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            696..812
                     /locus_tag="ACHJWB_004797"
     CDS             696..812
                     /locus_tag="ACHJWB_004797"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316260.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1031..2464
                     /locus_tag="ACHJWB_004798"
     CDS             1031..2464
                     /locus_tag="ACHJWB_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4316261.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          856 a          506 c          636 g          790 t
ORIGIN      
        1 tgtctttttt ctcaacccat ttttttacgc agacaaattt acccggggaa tacaaactgt
       61 aacgacagtt caccacacta tgggggataa aatgccagat caatccggac actgcaggac
      121 tccttcacgc atcttacgga tgcctgaact gagccaattg ttaggtatat ctcgttcgac
      181 aatttacgaa aaaatgaatc cgttatcgaa atactacgat gcaaccttcc ccaggccggt
      241 acgtcttggt agtggttctg tgggctggcg ttcatcagct attgacgaat ggcttacttt
      301 acatactgta cctgccagat cagcagtcaa aggtgttaat gacgaagtga gacatacgct
      361 ttcttcagat atgaccatga gcagataagt ttttgtcatc acgtgatcac ctcaggagga
      421 aaaagaagca taagaacaat ttagccagaa gtttaattga taaacagaat ttccagcatt
      481 cattgttgaa agagcagcta ttgtcgctat tgtagcggta attgctgcta taaccgaaac
      541 gtattacgaa aaaggaagca tttatgcctt acatcaaacc ggatatgtcg tattcagtat
      601 atgcatgacc atcaatgata gggaaggcaa taattaaagc tcaatacaat aaacaagtac
      661 ctctgactct ggttgcaatg tcagtattaa cagcgatgtc aattgcctgt cagaatcagg
      721 ttgacgtatg cagtcccgga aatttacgtg gggccgttaa tatttacact atggttctgg
      781 agaaagggaa aagaccgggg gaaaggtatt agtgaaataa tttccggagg tattaagaat
      841 gagaaatatg aatgatattt cgtggtgaat aatacagtct tcactgaatt aaccttatcg
      901 gtttattaaa catcagatct acaaatgctc tgtatttctc agagcatcag gtctgaatag
      961 cagctatttt cgctattgta gcgggatttg ctgctatttt actaacccat taatgaaaag
     1021 agaggctttt atgtgcttac ttgcaccaga aaatccttat ccgatatatg cactaccacc
     1081 gctggtgaga aatgcaataa ttgaaactca aaaaaataca caggcacctt tggctatggt
     1141 ggcaacatcc gcattaacag cgatctcaat tgcctgtcag aatcagattg acgtgtgcag
     1201 acctggaaat ttacatgggc ctgttaatct ttactctctg attctggctg attccggtga
     1261 aaggaaaacg accgtggata aagtgtttat gaaagcattt tatctcaggg atgaagccct
     1321 ggcggatgaa tacgcgaaac tggttgagaa ttacagtaca gaaaaggaaa tatgggagca
     1381 aaaacaaaaa gcgctggaat caaaatttca taaagagatt cgtgccggta aagattacaa
     1441 ggcaacagaa tcagagcttg aaacgcatct gaataaacct cctgttccgc cgcagatacg
     1501 tcgaacaatt tttaatgaga caacgataga gggaatgtta aaatattact ccgatagcaa
     1561 tcgctctttt gctcttgtat ccagtgaagg gggggtaatt tttgacagcc gggccatgag
     1621 taaactggga attattaaca ctttgtggga tggaggttct cttttcatcg acaggaaatc
     1681 atctcccgga attaatttga aggagccaag actgacgata tcggtgatga ttcagcctga
     1741 tgtttaccac aaaggttttt gtacgcgaaa aaaagaaatt gtgaaaacgt caggacatca
     1801 tgcaaggttt ttgatgtgtc aaccaacatc aacgcagggg acaaggataa taaccggaga
     1861 taattattca tcacagtatc aggatttgtt tgaagagagg atcaatgagc ttattgatga
     1921 aagtctggcg atgagtggtg aacgacgttg ccttcacttt tctcctcagg ctgccagaat
     1981 ctggacggat tattacaatg atgtggaatc gaagctgggg ggattggggc ctttaagaca
     2041 ttgccgggaa tatgctgcta aaaatgcaga gtatatggca agactggcag gacttattta
     2101 ccattcgagc ggtgaagagg gggagatttc cccttacact gcagaaatgg cgagagaatt
     2161 agcaatatgg tacggtaatg agtatgtgcg gttgtctaat ccgttaactt ttgacaactc
     2221 tgccctgacc gtacctgtgc gactcattcc agaggagctt gaacttttca actggataaa
     2281 aagctattgt attgagaagg ggatcctctg tatgaaaaaa aatgacattt tacagcgtgg
     2341 tccgaaccgt ttccggaaaa aggataaaat caactggtta ctggatttac tgtatgaaca
     2401 aaacagagtt gtaccggtta ttgagggaaa aacgttgtgt gtcgcaccta actttgacct
     2461 ctgaacgact taatcgttga gtacatatag tctcctctgc tacttgcgga ggagactaat
     2521 gtctttacca tgtcagcgtt gtattagaaa actctggtac aggtcagtca gtaaatataa
     2581 ctggtgtaac cactatttac tattggatag agtgttactc aggatattgt cggctttagt
     2641 ctgaggtgag gtggggcctt cattgttttc gtgttcaata aataatttag gttaattagt
     2701 attattagtg gaagttatgt gatctgttat gttgtcttgt acgcacttaa cactggttac
     2761 gtaccggaac actattagag tatgttat
//
LOCUS       DAZFAH010000333         2785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFAH010000333 DAZFAH010000000
VERSION     DAZFAH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.335"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..433
                     /locus_tag="ACHJWB_004799"
     CDS             <1..433
                     /locus_tag="ACHJWB_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316262.1"
                     /translation="FARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGS
                     HYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHA
                     ITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
     gene            443..862
                     /locus_tag="ACHJWB_004800"
                     /pseudo
     CDS             443..862
                     /locus_tag="ACHJWB_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            843..1808
                     /locus_tag="ACHJWB_004801"
     CDS             843..1808
                     /locus_tag="ACHJWB_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316263.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            1849..2271
                     /locus_tag="ACHJWB_004802"
     CDS             1849..2271
                     /locus_tag="ACHJWB_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4316264.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            2268..2757
                     /locus_tag="ACHJWB_004803"
                     /pseudo
     CDS             2268..2757
                     /locus_tag="ACHJWB_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145667.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          792 a          589 c          762 g          642 t
ORIGIN      
        1 gttcgcaaga aaaccagtgg gctgggccat gtcgttctcg ccggacagca ggctcaccat
       61 gaaagcgctg gaaatggcat gggaaacccg tggtaagccc ggcggggtga tgttccacag
      121 cgatcagggc agtcattata cgagcaggca gttccggcag ttattgtggc gataccagat
      181 cagacagagt atgagccggc gcggaaactg ctgggataac agcccaatgg aacgcttctt
      241 caggagtctg aagaacgaat ggatgccggt ggtgggttac gtaagcttca gcgaggcagc
      301 tcacgccata acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa
      361 cggtgggtta cccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc
      421 cagtttttgt tgaccacttc actgggcttt gaaagagcgc ccgaacatcg atgttcagcg
      481 tgtcacatat gaaggtggcg cgattgatca gcgagcgctt ggcgttaatc gagtgaagat
      541 atttgaacgc tggaaggcta tcgacaccag ggataagcgt gaaaagttca cggcgctagt
      601 gcctgcaatt atggaggcta tccggattaa tgatttcagg ttgtatcgtg aaattactga
      661 cggaaaaagc atcacgtgca tgatcgccgg gttaaacaaa gaatatggcg atgtggtgga
      721 gtccggactg ctttttgctg atcctgccgt agtggatcgt gaaactgacg aacttataga
      781 aaaagcaatt gctttcaaac ttgcgtatcg acagcaatac caacaaaaag ctggatggaa
      841 ttatgagcct tctttttgct gaacgcccac tggttataaa cacgcagctg gcaatgaaaa
      901 ttggcttaaa cgaagccatt gttttgcaac aactgcacta ctggttgaga gataccaact
      961 ccggcatgga atgtgatggt gttcgctgga tttataacac aacggaacaa tggctggaac
     1021 agttcccatt ctggtcagag tcaacgttaa agcgcgcgtt tgcaagtctg aaaacgctgg
     1081 ggcttttgcg ttgtgaaaag ctcaataaat caaagcgcga tatgaccaat ttctacacga
     1141 tcaactatgg gagcgagctt ttagatggtg gcaaattgag cgaatccatc ggttcaaaat
     1201 gcgccgctcc atcaggtcaa aatgacacga tggaagaggt caaaatgaaa cgctccattg
     1261 gttcaaaacg acccaatgtc atcgggtcaa aatggcctga tgatcttaca gagaatacaa
     1321 cagagattac tacagagaat aaaaacactt ttcgtccgga agcttcgcaa ccggacccgc
     1381 agacgactga acaggatttt ttaacccgga actccgacgc ggttgtgttt agtgcgaaaa
     1441 aacgccagtg gggtagcagg gaggatttgg cgtgtgcgca gtggatctgg gggcggatcg
     1501 tgggtctcta cgaacaggca gccagtgatg atggcgagat catgcgacca aaagagccta
     1561 actggactgc ctgggccaat gacgtgcgca caatgcggat gctggatggc agaagccaca
     1621 gacaaatttg cgaaatgttt ggtcgggttc agcgagatcc attctgggta aaaaacatca
     1681 tgagcccgtc aaagctccgc gaaaaatggg acgaactggt catccgcctg ggacgttcac
     1741 ctgtacagcg ttgtgttaat catatttctg aaccggatac agaaattccg cctggtttca
     1801 gaggataagt gttgatttca ggtcatgagg taattttaag ggggacttgt ggcaaaagtt
     1861 tttacacaag aagagcggga aaaaattaaa tggcaggtgg tggaactcgt gcgccagagc
     1921 ggccgtgaga cgttacggca actggaagct aaaacaggtg cgactagata tctgatgagc
     1981 gttcttgcca gagagctggt agccagtggc gatgtataca actccggcta cgggttattc
     2041 ccctctgaac aggcgcgtaa ggactggcaa aacgcccgca aaaaactctc gagggcaaag
     2101 gtgaagaaac ctgctgtggt tgatccggac cttatctggt catcacctga cggagaaata
     2161 cgtcgctacg acagtcgcct aaacataatc tgtcgcgagt gccggaagag cgaagttatg
     2221 cagcgcatac tggctttcta tcagggtaat tttcaggacg tggcgcagtg agtgcaccgg
     2281 caaccattct tgatatgtgc tgtggcagcc gcatgttctg gttcgataag tctgacgaac
     2341 gggcgatatt cagcgatatc agaaaagaag gatacacatt acgcaatggg agacgcttga
     2401 ttatcagccc tgacattatc gcagattttc gtgcattatc atttgcagac gcatcttttt
     2461 cgatggttgt attagaccct ccgcatcttg agagtgttgg tgataacgcc tggatgggaa
     2521 agaaatatgg acggctgaat aaagatgcct ggcgtgatga ttcgcgacag agatttaaag
     2581 aagcctttcg ggtgttgagg ccgcacggcg ttctgatttt ttaatggaat gaaacgcaaa
     2641 taccggtaag ccagattctg gcactgacag acagaaaacc tgttatctgt caacgaacag
     2701 ggaaaaaacg ataaaaccca ctggattatt tttatgaaag aggcatccag tgagtagatt
     2761 cgtaaggcta cagatacgta tatct
//
LOCUS       DAZFAH010000334         2743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFAH010000334 DAZFAH010000000
VERSION     DAZFAH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.336"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..2355
                     /gene="ydeP"
                     /locus_tag="ACHJWB_004804"
     CDS             76..2355
                     /gene="ydeP"
                     /locus_tag="ACHJWB_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO4316265.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMAVLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMITLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2603..>2743
                     /gene="safA"
                     /locus_tag="ACHJWB_004805"
     CDS             2603..>2743
                     /gene="safA"
                     /locus_tag="ACHJWB_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4316266.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISE"
BASE COUNT          731 a          639 c          675 g          698 t
ORIGIN      
        1 gataatccct ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc
       61 acacaggata agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg
      121 ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccaggatgtt
      181 attgccatgt ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca
      241 gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg
      301 gaagtcacgg ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta
      361 cttacctggg gagatcacga gcttgaggct gcgggacgtc tcactcagcc tttgaaatat
      421 gatgccgtca gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc
      481 gcacgccttc aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact
      541 tccaatgaag ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt
      601 cccgactgct ccaacatgtg ccatgaaccg accagtgtgg gtttggcagc gagtatcggc
      661 gtgggtaaag ggactgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt
      721 gggcataacc ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa
      781 cggggagcga aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc
      841 gcaccgcaaa acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac
      901 tacaacgtgc gcattggtgg cgatatggcg gtgctcaagg ggatgatgcg cctgttaatt
      961 gagcgcgatg atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt
     1021 caaacgcata ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa
     1081 gatatcgaac gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat
     1141 gccgctgccg atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt
     1201 acccagaatg tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct
     1261 ggtgcgggta tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt
     1321 atcaccgaga aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc
     1381 ccacctcatg cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag
     1441 gctcgagcat tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg
     1501 agcgctgtac cgttaacgca attagatctg gcggtacacg tagccactaa gcttaaccgc
     1561 tctcatctgc tgaccgcacg gcatagctat attctgccgg ttctgggacg tagcgagatt
     1621 gacatgcaaa aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat
     1681 gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc
     1741 gcgggaatcg cgcaggcagc actaccccag agcgtggtag cctgggagta tctggtggaa
     1801 gattatgatc gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgactataac
     1861 cagcgcatcc gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg
     1921 atgacgccgt caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct
     1981 tcagcgttta acagtaagct ggtcatggcg acagtacgca gtcatgatca gtacaacacg
     2041 acgatttatg gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt
     2101 atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt
     2161 gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt
     2221 taccctatgg ctgaccgctc actggtgacc tattttccag aatcgaatca catgataaca
     2281 cttgataacc acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta
     2341 gaaccatcaa attaatgcct cttctcattt cttctgctgt catccgcaca gcagaagaat
     2401 tcctcattga ctattatcgc gcaatttgct cacatggatt aaattaaact acatactata
     2461 agatataaac ttctgcctac agctgtaaga aactccgctc agtactgaag caccagtcct
     2521 atttcctctt ttttccagcc tgttatatta agcatactga ttaacgattt ttaacgttat
     2581 ccgagaaata aacatatttg aaatgcatgc gaccacagtg aaaaacaaaa tcacgcaacg
     2641 agacaactat aaagaaatca tgtctgtaat tgtggttgtc ttattactga cacttacgtt
     2701 gatagccatt ttttcggcaa ttgatcagct gggtatttca gaa
//
LOCUS       DAZFAH010000335         2743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFAH010000335 DAZFAH010000000
VERSION     DAZFAH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.337"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..531)
                     /locus_tag="ACHJWB_004806"
                     /pseudo
     CDS             complement(1..531)
                     /locus_tag="ACHJWB_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(609..920)
                     /locus_tag="ACHJWB_004807"
                     /pseudo
     CDS             complement(609..920)
                     /locus_tag="ACHJWB_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(917..1436)
                     /locus_tag="ACHJWB_004808"
                     /pseudo
     CDS             complement(917..1436)
                     /locus_tag="ACHJWB_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            1438..1521
                     /locus_tag="ACHJWB_004809"
                     /pseudo
     CDS             1438..1521
                     /locus_tag="ACHJWB_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(1648..2424)
                     /locus_tag="ACHJWB_004810"
                     /pseudo
     CDS             complement(1648..2424)
                     /locus_tag="ACHJWB_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          677 a          744 c          706 g          616 t
ORIGIN      
        1 gttatgttga gtttcacata acgggtccag cactatcgca ttcgggtgtg tgaagctctc
       61 aatcagcggt tgcaggctgg taacgggctt ttccgtcggg tgatggcggt tgcccgaata
      121 tttccagccc agcacgtccg gcagcgggtt ttgtagcaga cgtggacggc cttttgccag
      181 gatgtaggcg cattcgtggc gatagcccac atatgcggcc ttcgatgtgt agtttttggt
      241 gaagacctag tgaccaacaa cgctgaatcc cgcatttttc caggcagaca taaaacgctc
      301 gacgcggttc cagccataga agctcaccat cagcgcgtct tttttgagta cgcggtacat
      361 ttcattacag gccggttgca gccattcatc ggttttatcg ccggcaatgg tacgcccgga
      421 acggtcacgg aaaccgacga gatatggcgg ggcggtgagg atgaaatcaa tggcgttatc
      481 agggatacgg gccataacat cgatgcagtt gccgaggaca aaacgggaca taacaacact
      541 ccttatctta cagagggcac cgggaggccc ccccctgtgg tgcccgctcc ccgctgcctg
      601 tgggctgttc accgcgtccg gtaacgggtc atgccgaccg tggaataccg cagccgtcgt
      661 ctttttgacg gcaaggttat gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc
      721 gacaggctgg cgtacactgt caccgatgcc agccgggagt ggtaaaagcg cccgtcccgt
      781 gcccgccgga cgcgggacac tgaccacagg ctgcgttcct atcgtgccgc gtcagccggc
      841 gcagtaaaaa gaggagcctg gcgacatcct ttgtcggaaa agaaacgggc ggttgcccgc
      901 ccgtctccgt catacttcat ccgccataat gcgccacatt gtcgactatc cacgcaggtc
      961 cggtgacttc cggcaactcg tcatcatctg tcagatgtgg ccattccagt tcgccccaca
     1021 ggtcacttca cgccatactg ttccgtatac cagttatatg cagaggggaa catctttcag
     1081 aacaccaccg ttaaagccgt tcacttcaac atcctggcgg gcgggcagaa ccgtcagcaa
     1141 ttcaaacggc atgttttttt ctggcagcac attgtcacac agacggttcc accacactga
     1201 aacatcctcg ttactccaga agtcacacca gtcacctctt ttgacggtga aatgcacggt
     1261 gatggtttcc ccgacatcat ccggtaatcc ttcccagcga cgctggccaa taccgctctg
     1321 taaccagagt tcatgcagtt ttattcatcc agtagcacac cgttctgcag gtgcgtcagc
     1381 cagcgggtga acgcgagatt ctccggcgat aaaacgccac gtccggcagc cgtcagggca
     1441 acagagtggc agatgaagcg tgagatgttg tcaccggcat acactacccg atggaacgaa
     1501 ctaccgatcg ccagactttg aaaaaaaatt catggtgatt tcactatgaa agaatttaaa
     1561 aaaatacttt atattcagtg aaataagcat gaaaaaagcg tggtgaaaat tgcaataaat
     1621 atcctccgac atagccctgt ctcttgatca gatctcctga tcaagagact tcatcaccag
     1681 gtaaccctca accatatcct gaagtctgaa ccagccatcc cacataacta cccaaccggg
     1741 gcggcctatg cgtttgctgt catgccatcg ccccagttta gccagtttca gtcaggccca
     1801 tttcaggggc ggtgcctgcg ccggaagcgg ctttccttcc agcttaaccc acagcagttc
     1861 cactctgtcg gcgtcagtat tttctcatag ctgtcatttt gtgtttcttc actgacgcct
     1921 ccctgccgca ggcccagcac ccgtaccgcg ataaacgcct tgataaccac catgcgctca
     1981 aggttatccc gggtctgcat tcgcagcgat tccacacatg taccaccact tttccacgcc
     2041 ttgtggtatt cctctattag ccagcagccc gcgcgtcttc gggttagtgg tggtattccc
     2101 atggtcatca aattccgtac tgtggctgaa gttaatggtg gtggtgtctt ccagtacgag
     2161 gagcagcgga tgtgcctcac atgcccttgc agtggcggta aatccggctt cggcaatgga
     2221 ttgcggggac acagacgggt tacgtatcag gcgatacgca ccttcaacct gagcagtgga
     2281 ctgagatgat ttcacaatag aaaggcctgc atgctgagcg agagaagagg cctgtgacac
     2341 aaggcgtcgt gtacgacgcg gatcaccgcg acgggtatgt ccaaactgct cgttagccca
     2401 tgaataacaa tcagaaagta ccataacaga cccgaagaaa atgaaatata agagaagatc
     2461 aacgggtgaa gagaaagttc aaaaaatcgc tgccggggag gaaggaaact accggattga
     2521 aagagtcccc ccctaaaaca gagtgacaga caccacaaat ccccgggtgg ggatttgtgt
     2581 ataagagaca gagcctagct gggggttctg tgcacaaaaa ggacgccatt tggcatgagc
     2641 ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caatccaaca tcaaaagcag
     2701 gttaatttat ggacgaaaaa cagttgcagg ctctggctaa cga
//
LOCUS       DAZFAH010000336         2634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFAH010000336 DAZFAH010000000
VERSION     DAZFAH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.338"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1181
                     /locus_tag="ACHJWB_004811"
     CDS             <1..1181
                     /locus_tag="ACHJWB_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4316267.1"
                     /translation="VMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRY
                     NAAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDG
                     MHDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASS
                     FAGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQ
                     DNPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRT
                     WPVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQ
                     LPGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG
                     "
     gene            1165..2289
                     /locus_tag="ACHJWB_004812"
     CDS             1165..2289
                     /locus_tag="ACHJWB_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4316268.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2279..>2634
                     /locus_tag="ACHJWB_004813"
     CDS             2279..>2634
                     /locus_tag="ACHJWB_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4316269.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGR"
BASE COUNT          545 a          715 c          818 g          556 t
ORIGIN      
        1 ttgtgatggg ggatgatgtt cagacacagc gaaatcgtct gcgtgatgcc cttcgtgctc
       61 ccggtgccgg ggagctgctt cacccggatt acggcacgtt aaatgtgctg ataaacagct
      121 ttgaaagtcg ttataacgcc gctgaacagg gcgtggttga gttcaccatt aatgtcacgc
      181 cggtaagtga tgataccgca cctgctgtca cacaggacac ggccgccatt ctggagcaga
      241 agagtaccac cgcgctcggg caggtttttg aaaccctgga agcgggctgg acggtgatct
      301 ctgacggaat gcacgatgtc caggcaatga ccgaaaccat cagtgataag gtgtccgccc
      361 tggaaaatgc cgtttccggc atggggattg tacaggatat cagtgccttt actgccacgt
      421 tcacggcgct gaaagggaat gccacggcat tactgacagc accttcccgc atggcctcat
      481 cttttgcggg gcttttcagt gccctgatca ccctgccatc gctgccgtca ctgtcttcag
      541 gaggcctgaa tacccggccc ggtggcagta tcggccggac gacatccgcc gtctctcagg
      601 gaatgccgca actgtacagg acattatctt ccctgcgtta cgtgctggat gagcaggata
      661 atccgcagac tctcatcggt ctgacaccgg cagcacaaaa gaatattcgt ctgatacggg
      721 cggtaatgca gagtgctgcc gtggtggccc aggcacagac cgtgggaaaa ctgctggatc
      781 aggttctcag tcaggaaacc ctgccggaca gtgacgcagc ccaccgtacc tggcctgtct
      841 ggctggaaag ttcagttgat cttcagcgca ttaaccgtga cttaagcgaa gcgctcgaac
      901 ggcaggtgat gacactgtcc gggcaggggt ataccgccac ggcgctgacg cttcgtgatg
      961 ccagactggc actgacagag gatctgaata cacggggagt tcagctaccc ggtgcaacag
     1021 tagtgactgt acgtaccacc gagcctgcac tggtgaccct gtaccgtgcc accgggaaca
     1081 gtaccggctg gcaacgtttt gtgcgccgta acggtattgt tgatccgctg ttcattcccg
     1141 gaggccattc agtggaggtg attagtgagc agcagggttg aactgtatct gggcggtgag
     1201 attttttccg gctggctgac ggtgagtgtt cgtcgctctc ttgaacatct ggcgggctcc
     1261 tttgaactgg gggtaatgat gcccggtgta cgccttccgt catccgtccg tgccggtcag
     1321 tctctggaat tgcgcattga cggtcagcct gtgatcactg gctggctgga tcaggtccgg
     1381 cagcgcatca gcgccacgcg ttttcagatc acgctcagcg gacgggataa aaccggtgac
     1441 ctggtggact gttcagccat tcatccgggc agccagtgga ggaaccgcac gctggagcac
     1501 attgcttcag atttgtgtgc tccgttcggg gtcacggtgc gctggcaggt aaatgatgca
     1561 acggcagccc ggcccttttc caccttcaca ctggaaaatt cagaaaccgt ggcagatgcg
     1621 ctgacacggg ccgcgagaca cagaggcgtc ctggtaacca gtaatgccgc cggtgaactg
     1681 gttttcactc aggccggcag tcagcgtggc gacacgctga cgctgggcga aaatctgctg
     1741 gatttggatc acaacgtgga tcaccgtctg cgccacagtg aataccgtgt acgggggcac
     1801 gggcgtggtg gtggtcatgc cggggatgca ctgacagccg gaacgctggc cgcaccagtt
     1861 ggtacggtga cagacagtgc catccaccgt tacagaccga aaattgtgct ggcggatcat
     1921 gctgttgatg cagacggtgc acgccagagg gctgtccggg aaatgcgccg ggcggttgcc
     1981 cgctctgtgc gcctgacagc caccgtgcgg cactggtttc gggagaacgg ccagttgtgg
     2041 gatatcaacc tgctgacggc tgtcacggct ccccgcaccg gagtggaaga gcgtgatctt
     2101 cttgtctgtc aggtggagtt ttcgctggat gcaaatcacg gcgaaaccac ccgtctgatt
     2161 ctggcacccc gtgacggctt tattgttccg gcagagccgg gaaacagcgg aagcggaaat
     2221 gcgggtgacg tggacgcctt cgtgcgggca cagatgaaaa aacaggggat taaattcaat
     2281 gatgaatgac gaagtcatca gccgccttct ggcccccgtg atgcggggtg ttcgtctgct
     2341 gttcgggcgt ggtgtactga ccggcacaac ggacacgctg aaaatccaga atgtgcaaat
     2401 caccggtatg gatggtgaaa cctttgatga cgttgaacgc ccccagcagt acgggcaaat
     2461 cagcgtcccc ctgcctggtg cggaagtttt tctggcctgt gctggcggac aacgggatca
     2521 ggccgtggtg cttgtggtgg aagaccgccg cagtcgcccg accggactta ccgccggaga
     2581 taccggcgtg tatcaccatg aggggcaccg tatacgtctg acaaagaacg gccg
//
LOCUS       DAZFAH010000337         2624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFAH010000337 DAZFAH010000000
VERSION     DAZFAH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.339"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..502
                     /locus_tag="ACHJWB_004814"
     CDS             <1..502
                     /locus_tag="ACHJWB_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4316270.1"
                     /translation="CISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATELSAIHGH
                     STSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDT
                     RKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLL
                     AGLPAH"
     gene            complement(512..1939)
                     /locus_tag="ACHJWB_004815"
     CDS             complement(512..1939)
                     /locus_tag="ACHJWB_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4316271.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1936..2622)
                     /locus_tag="ACHJWB_004816"
     CDS             complement(1936..2622)
                     /locus_tag="ACHJWB_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4316272.1"
                     /translation="MLFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDDRD
                     DLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGYTF
                     GRQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQPLAYYSAC
                     PETLKDMSDL"
BASE COUNT          742 a          639 c          595 g          648 t
ORIGIN      
        1 ctgtattagc caacccaaga aaacaacgtt gcgtgataaa gccatggtga attatgcctt
       61 tgattattta agctcaccag gcagtctacc gttaaccacg gcagccacgg agctttccgc
      121 gattcatggt cactcaacat cgcaatatcg ccttggcgaa ttttatcttc atggtagcga
      181 cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag cagaacagga
      241 aaatccgcgc gcacaaagca aactggggtg gatctacctg aaaggcctgg gagtcaaacc
      301 cgatacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc
      361 tcaatatact ttgggtttga tctacagaaa tggcactggt attaatgtta accattatga
      421 atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact
      481 tgccggactt cccgcacatt aacgtcgact gtcacccgaa gagatggagt attttcccta
      541 tcaaaccggc aaagataaaa atgtagaaaa taattttgat gacgcccaga cagcccagcc
      601 cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg
      661 taaaatttgc cgcctgaatt aataatcgac gaataattaa tgcttccagt ggacaacgat
      721 cttctgcatc aaggcggctc acaatcggca ttaatgattc ttctgtcggt tggctggcct
      781 gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta tcacgcgtga
      841 gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa cgccccagcg
      901 cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc
      961 cggggacata aagtagcggt tttcccttat cgttaaccag atcacgcgct tcaatcatca
     1021 tcaccactaa tgggtaataa tcgacccgat gctgacggca ccattgcaac attaaagcat
     1081 ccgcctgggt ggagggatac tcgcgccagt agtcacaaag ttctgccagc aagctttcgc
     1141 cttctccaca atagacgcgt tgcgcgtatt cataataacg cgcatcttca ttatcaggtt
     1201 cagcttcccg ccacgaccgg gcgtaatcca gaagttcggg gatatccagg cgacctgcgc
     1261 tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat
     1321 gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga gcgaacattt
     1381 ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag atgcagaatg gtctggaagt
     1441 taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct tcgtctattt
     1501 ccccgacgtt ttcctcctgc aaccaccgca atctttctgc taataacacc acgcactcga
     1561 aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat tcatcaatat
     1621 cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac
     1681 gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt tctgctacct
     1741 catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgg agcgcctcct
     1801 catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga aaggacggta
     1861 acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct atttcgagga
     1921 tcttccagca atttttcata ggtcgctcat atccttaagc gtttccgggc atgcagaata
     1981 gtaagccaat ggttgccagg acaaggtagg gtaatacgca ttatcactgg caaataattg
     2041 ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt cacgtaataa
     2101 ctgcagatta tcatcctcgg gagactgcca atattgccga ccaaaggtat aaccggcaaa
     2161 gtattgccgc cagttacgat aataacgtag agcccgtaga tgaatgcgcg actggatcca
     2221 tagactttct tcttcgctca accagttatt aagtacgccc attcggctta aaaaacccat
     2281 ccggacataa tcccaggctt taattcctcc ctcaccacag cattcggcgg tgctggcgac
     2341 aaattgcgca taagcctgac cttgctcatt tagttcagca agatagttac gccattcaca
     2401 tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat gaccgttctc
     2461 gatcattcgg tgaattgtgc gcagcaggtc atcacggtca tcaattcccc atgaggaaga
     2521 aaggtcaaaa aagcatgtac ctaaatacat tttgggtgat gaatgcgaag gtaaacagca
     2581 ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attt
//
LOCUS       DAZFAH010000338         2604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFAH010000338 DAZFAH010000000
VERSION     DAZFAH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.340"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..843
                     /locus_tag="ACHJWB_004817"
     CDS             <1..843
                     /locus_tag="ACHJWB_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4316273.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
     gene            1183..2001
                     /locus_tag="ACHJWB_004818"
     CDS             1183..2001
                     /locus_tag="ACHJWB_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4316274.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2092..2577
                     /locus_tag="ACHJWB_004819"
     CDS             2092..2577
                     /locus_tag="ACHJWB_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4316275.1"
                     /translation="MTTVSHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT          619 a          665 c          702 g          618 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaaaatattt tcagatatat attatctggg tgatggttaa gatgaattta tttaattttg
      961 ggggaagata caaaaacaac cagtgaacat atttccggcg ggttatccgc aatgctgaag
     1021 gtcagttgca ctggcgatgc cggaatacag cttaatacgt ggttcgccag tgaagggctt
     1081 ctcagacaat aaacctcatt catttcaccc atcaggccgc gtcttctccg ggagacgcgg
     1141 ctttttcatt tatatcgctt aatcattcat aaggagcaaa tcatgagatt agccagtcgt
     1201 tttggttatg cgaaccagat acgtcgtgat cgtccgctga cacacgaaga actgatgcgc
     1261 catgtaccga gtatttttgg ggaaaaccga catacctctc gcagtgaaca ctatgcgtac
     1321 attcccacta tcaccgtcct ggaaaatctg cagcaggaag gctttcagcc gttctttgcc
     1381 tgccagaccc gcgtgcgcga ccagagccgc cgggaataca caaaacacat gctgcgtctg
     1441 cggcgggccg gacagataac cggtcagcat gtccctgaaa ttattctgct caactcccat
     1501 gacggttcat ccagctacca gatgttaccc ggatattttc gtgccatttg taccaatgga
     1561 ctggtctgcg gtcagtctct gggagaattg cgtgttccac accggggaaa tgtggtggac
     1621 agagtcattg aaggggctta cgaggtggtg ggcgtttttg accggataga ggagaagcgt
     1681 gatgccatgc agtcgctggt cctgccgcca ccggcacgcc aggcgctggc acaggcggca
     1741 ctgacttacc gttatggtga cgaacatcag cccgtcacca ccgccgacat tctgacacca
     1801 cgacgccggg aggattacgg taaggacctg tggagtgctt atcagaccat ccaggagaat
     1861 atgctgaaag gcgggatttc cggtcgcagt gcaaaaggaa aacgtatcca tacccgggcc
     1921 attcacagca ttgataccga cattaagctc aatcgcgcat tgtgggtgat ggcagaaacg
     1981 ctgctggaga gcatgcgctg atgccgtttc actgaaagtg cagtcctgac tcacatctgt
     2041 tcctgccccg gggcttcttc cattcattta ctttttataa ggaataacct catgacaacc
     2101 gtttcacata attccaccac atcttctgtt tccgtaaccg ctgcatcagg gaatgaccag
     2161 cctcagttgg ttgccaccct cgtccctgat gaacagcgca tcagcttctg gccgcagcac
     2221 tttggcctca ttccacagtg ggtgaccctg gagccccgtg tcttcggctg gatggaccgt
     2281 ctgtgtgaag actactgcgg tggtatctgg aatctgtaca ccctgaacaa cggcggggca
     2341 tttatggcac ccgaaccgga tgacgatgat gacgaaacat gggtactgtt caatgccatg
     2401 aacggtaacc gcgctgaaat gagtccggaa gctgccggca ttgctgcctg tctgatgacg
     2461 tacagtcatc atgcctgtcg tacggagtgt tatgccatga cggtccatta ttaccggctg
     2521 cgggattacg ccctgcagca tccggaatgc agcgccatta tgcgcatcat cgactgaatg
     2581 gagagaagta caatgcaaca gctt
//
LOCUS       DAZFAH010000339         2587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFAH010000339 DAZFAH010000000
VERSION     DAZFAH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.341"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..713
                     /locus_tag="ACHJWB_004820"
     CDS             <1..713
                     /locus_tag="ACHJWB_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316276.1"
                     /translation="CKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDLTLLQL
                     RRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTAT
                     LLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERKVLRLL
                     GKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVY
                     GDAAEWNTAELRREMSHS"
     gene            671..1348
                     /gene="bglJ"
                     /locus_tag="ACHJWB_004821"
     CDS             671..1348
                     /gene="bglJ"
                     /locus_tag="ACHJWB_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4316277.1"
                     /translation="MEHSRIKKRNVALIEKYVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1386..2174)
                     /gene="fhuF"
                     /locus_tag="ACHJWB_004822"
     CDS             complement(1386..2174)
                     /gene="fhuF"
                     /locus_tag="ACHJWB_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4316278.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2315..2551
                     /locus_tag="ACHJWB_004823"
     CDS             2315..2551
                     /locus_tag="ACHJWB_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4316279.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          680 a          571 c          651 g          685 t
ORIGIN      
        1 gctgcaaaaa tggaattgtt atcagtaaaa tacctgttat gcaagcaggg ctaaaagagg
       61 tcatgaggac tcacttccct gaatatgaaa taatatccag cgcctctgcg gaggacctta
      121 ccttattaca attacgtcgt tccggattag tcattgctga tttagccggt gaaagtgaag
      181 atccacgttc tgtttgtgaa cattattatt ctttaatctc acaatatcgg gaaattcact
      241 gggttttcat ggtgtcacgc tcctggtatt cccaggcagt agaactgctc atgtgcccta
      301 cggcgacgtt attgtctgat gttgaaccca ttgagaatct ggtcaagacc gtacgttccg
      361 gcaatacgca cgcagagcgt atcagcgcca tgctgacctc tccggcaatg actgaaactc
      421 atgattttag ctatcgctcc gtcattctca ctctttcaga gcgcaaggta ctgcggctat
      481 taggtaaagg atggggcatc aaccagatag cttcattgct taagaaaagt aataaaacta
      541 tcagcgccca aaaaaacagt gcgatgcgtc gactggcaat tcacagcaac gctgaaatgt
      601 atgcatggat aaatagcgcg cagggtgcaa gagaacttaa cttgccttct gtttatggag
      661 atgccgcaga atggaacaca gccgaattaa gaagagaaat gtcgcactca tagaaaaata
      721 cgtcatgagt agtatcggta ttgagagttt attcagaaag tttgcgggta gcccttataa
      781 gctccatacc tataccagtc aggagtcatt tcaggatgcc atgtcgcgga tctcgtttgc
      841 ggcggtcatt ttttcttttt ctgccatgag aagtgagcgc agagagggat tatcttgcct
      901 gactgaactg gcgattaagt ttccgcgtac ccggcgttta gttattgcgg atgatgatat
      961 tgaagctcgg ctgattggtt cattgtcgcc atcaccgctg gacggtgtat taagtaaagc
     1021 gtcaacgctg gagatttttc atcaggaact ctttttgtca ttaaatggtg tacgtcaggc
     1081 gaccgaccga ctgaacaatc agtggtacat taaccaaagc cggacgctaa gcccgacgga
     1141 gagagaaata ttgcgcttta tgtcgcgtgg ctactcaatg acacaaattg ccgagcagct
     1201 taaacgtaat atcaaaacga tccgtgcaca taaatttaat gtgatgtcga aactgggcgt
     1261 cagttctgat gcagggttgt tggaggccgc agatattctg ttatgtatgc ggcattgcga
     1321 aacaagtaat gtgttgcatc cctattaatc cgcatgatgc cgggtttact tccccggcag
     1381 tgctttcatt tcagcgtaca atcgccacat tgctgcacat ccggtaagcg ataacgctgg
     1441 cagcaagtgc ggcgcaccag caggccatcg cgcagtacca cggtacgcca gagtggatta
     1501 tcttcaccgt tcgtgagcgt tttctcaaaa aagagggcat ggcgcagcga ttcaaccgtc
     1561 gcctcgccga gcagttgctt catctcagta agataccagt tgatcaaata accggtatta
     1621 ctccagataa gtttgccgtt gatctctccg gtcgcttcta gtgcctgcac aaccggaacc
     1681 agcgcctggc tgattaacct ttccattcgc tgctgcggcg aatgtggtgt tgcgttttta
     1741 tcttcacaca catcgaccca gaaacaggcg gcacggccgg tttcgtgaaa ctcagcatgg
     1801 aaatgttccg gcgacacatc taatgccttt tcctgcgtca gtagcgccag cattaatggt
     1861 ggcaccatca ggccgatata ccattgtgcc catagtgaga tcagcggttt gttctcgcgg
     1921 atcatcgttg gttggttgcg atagatatga tcggaataga ccgcgagtag agaacttagc
     1981 gcattcggtg atgaccattg cgccagcgtc atggcgttaa gcggagcggg ttcatccagg
     2041 cggataaact ccagcaaatg ttcacgatgt tcggcgatcg tcgcacgtac ggcttgtgca
     2101 agcccagcat cctgtggctg gagatgcgtt cgccagataa catcttcata gagcggtgcg
     2161 gaacgatagg ccataatcgg gatagtaatc taaatgataa tgattgctaa tcctagcgat
     2221 aggtttaccc gatagcaagg gatttctctg gcttgcaaat gataaaaatt atcatatgat
     2281 attggttatc attatcaatg aaagagatga aaccatgttg caacgtacgc tgggcagtgg
     2341 ctggggagtg ttgctgccag gattgctgat tgcagggctg atgtatgcgg atttatcacc
     2401 ggatcagtgg cggattgtca ttctgatggg attagtattg acgtcggtga tgctgtatca
     2461 caaacagtta cggcattacg ttttgctacc atcgtgcctg gcgcttattg ctgccatcat
     2521 gctgatgata atgaatttga atcagggatg aaaaatcaag gaagaaacaa gaaaggaagt
     2581 aaagata
//
LOCUS       DAZFAH010000340         2586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFAH010000340 DAZFAH010000000
VERSION     DAZFAH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.342"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            439..645
                     /locus_tag="ACHJWB_004824"
     CDS             439..645
                     /locus_tag="ACHJWB_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316280.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(901..1173)
                     /locus_tag="ACHJWB_004825"
     CDS             complement(901..1173)
                     /locus_tag="ACHJWB_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316281.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1333..1866
                     /locus_tag="ACHJWB_004826"
     CDS             1333..1866
                     /locus_tag="ACHJWB_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316282.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2087..2200
                     /locus_tag="ACHJWB_004827"
     CDS             2087..2200
                     /locus_tag="ACHJWB_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316283.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2202..2525
                     /locus_tag="ACHJWB_004828"
                     /pseudo
     CDS             2202..2525
                     /locus_tag="ACHJWB_004828"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2512..>2586
                     /locus_tag="ACHJWB_004829"
     CDS             2512..>2586
                     /locus_tag="ACHJWB_004829"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO4316284.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGII"
BASE COUNT          668 a          522 c          705 g          691 t
ORIGIN      
        1 gccagcgact gacctgaaag aagacgctgg ctaaaaggcc ttatatgttt gtagagactt
       61 atttttcaca gacagcaatg atgcctgtca atatattatc aatatgcgga ttgtttcagt
      121 tacagatgct ttattaagga aaaaaacagc cagcactgac tttcggtgga gaggtgctgg
      181 ctcagaagga tagttggatt tcacatgata cttatgcctg gcggtatatt ttctgacaga
      241 cagtgacggg tgttgtcaag atattgtgtc atttataacc tgaatcaggg ggtggccgga
      301 atgttatctg gcatttttag cagagcctga atgccataat cacggctccc ggagttggcc
      361 gtcagtgggt gacactggcg gcttttttgt ttttctttac tttcattttc tgtcggcggt
      421 gacggagaca tacatcagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg
      481 gtggggacgg gatactggtt actgcagctg ctggacaaag tctctccgtc ccagtgggtg
      541 gcaataggtg tgctgggaag tctgctgttt ggcctgctga cgtatctgac taacctgtat
      601 ttcaaaatca gagaggaccg tcgtaaggtg gcgcggggag agtagtcgat gaataaacaa
      661 tacgaactgg ttgtaaaatg aatatttcta actgaaaaaa cgttccatga ggtaagaaaa
      721 ggtcacaggc aatcaataac aggacgtgat gaaagaccct tgcatttgtg cgctttctct
      781 ttagatagca gcagatactg aaaatctgag ttgtcgggga gtcagggata cagctgtgca
      841 agagttggtc attgtgattc cattgaaatc ctgtatgcca tgaagggcag gattttatgg
      901 ctacctgagc tttggtgata gtaagttgaa aattcgcatt ttttgctgac atgcgtaacg
      961 agaatcccat aaacagggag gacttaattc ttcattaacc catgcgttga tattatgttt
     1021 cagccgttga agcatcagcg gtgttaatgt tgtggtaata atatccagcg ttttatgtga
     1081 gatcttaccg taagggtctg caagaatgct gcttgttgct tcgttattat ctgccatcag
     1141 aagaagtaac tctgatttaa cgttttctgt cattagttgt aaaaatcttc tgcgcaaact
     1201 ttctttactg ttcatttata tggcttcatt tgttgtaatc tgctgcgtct caagggatat
     1261 gtttatgaga gcgaccatga gtgtttgatt atatacctaa catatcaagg gattagaaat
     1321 cgataaatcc ccatgaacga aaaaataaaa tacggcctgt cggctgccgt tctggcgctg
     1381 attggtgcag gggcgtctgc gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt
     1441 aaccacacca cggcataccg tgatggtgcg gggatctgga ccatctgccg tggtgccatt
     1501 ctggtggatg gtaagcctgt tattcctggc atgaagctgt caaaggaaaa atgcgaccgg
     1561 gttaatgcca tcgaacgtga caaggcgctg gcatgggtgg agaaaaacat ccgggtgccg
     1621 ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggccccggt
     1681 aagtgcttcc cgtcgacgtt ttataaacga attaatgcag gcgatcgaaa aggtgcctgt
     1741 gaggcgattc gctggtggat taaggacggt ggcagagact gccgtatccg ttcaaataat
     1801 tgctacggtc aggtctcacg gcgtgaccag gagagcgcgc tggcgtgctg ggacatcgac
     1861 agatagcaga atattttcct gaaaaatgac gttggccaac gcgggtggat aacacgaaat
     1921 cctgaaaact ggtaaaacct aagtgaataa aagtaaaaac cccgtttgtt ggcagcaagc
     1981 ggggttttgt gttttctgac cttgagtaag gcaagggaga aattatgggt agggaggtac
     2041 tttccctgtg aggaagtata aaagattctt tctgaggttg tccattatga aaggcattga
     2101 agtggagacg ccagccagtc tggatttaac aagagcggca gcttttgcca ttcgtattgt
     2161 ggccattgct gttctggtct gggcaatccg ttggtggtga tatgaaccgt gttctgtgcg
     2221 tggttatcat tgtcctgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc
     2281 gtgatcacgc catcatctac aaagagcagc gcgataaaaa agccagtgag ctggagctgg
     2341 cgaacgcgac aattactgat atgcagatac gccagcgtga tgtcgctgca cttgatgcca
     2401 gatactcgag ggaattagcc gatgcgagag ctgaaaatga aactctgcgt gctgatgttg
     2461 ccgctggtcg taagcgcctg cggatcaacg ccacctgctc cggtaccgtg cgtgaagcca
     2521 ccggcacctc cggcgtggat aatgcaaccg gcccccgact ggcagacacc gctgaacggg
     2581 attatt
//
LOCUS       DAZFAH010000341         2568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFAH010000341 DAZFAH010000000
VERSION     DAZFAH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.343"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..997
                     /locus_tag="ACHJWB_004830"
     CDS             263..997
                     /locus_tag="ACHJWB_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO4316285.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSIQVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            994..1494
                     /locus_tag="ACHJWB_004831"
     CDS             994..1494
                     /locus_tag="ACHJWB_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4316286.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1568..2140
                     /locus_tag="ACHJWB_004832"
     CDS             1568..2140
                     /locus_tag="ACHJWB_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4316287.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTVIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
BASE COUNT          699 a          643 c          676 g          550 t
ORIGIN      
        1 gtgaacagac ggtgaacagt cattactgcg attgttcacc atttaactta ctgtattact
       61 tatctttttt attaaggtga acagaggtga acagtaaaat ataaaaaaac aaacagtaag
      121 ccggtttttc ctgcgacctt ttcctggctt gccggtctga ggatgagtct cctgtgtcag
      181 ggctggcaca tctgcaatgc gtcgtgttgt tgtccggtgt acgtcacaat tttcttaacc
      241 tgaagtgacg aggagccgga aaatgtctga caacaccatc cctgaatatc tgcaacccgc
      301 gctggcacaa ctggaaaagg ccagagccgc ccatcttgag aacgcccgcc tgatggatga
      361 gaccgtcacg gccattgaac gggcagagca ggaaaaaaat gcgctggcgc aggccgacgg
      421 aaacgacgct gacgactggc gcacggcctt tcgtgcagcc ggtggtgtcc tgagcgacga
      481 gctgaaacag cgccacattg agcgcgtggc acgccgggag ctggtacagg aatatgacaa
      541 tctggccgtg gtgctgaatt ttgaacgtga acgcctgaaa ggggcgtgtg acagcacggc
      601 caccgcctac cggaaggcac atcatcacct gctgagtctg tatgcagagc atgagctgga
      661 acacgccctg aatgaaacct gtgaggcgct tgtccgggca atgcatctga gcatccaggt
      721 acaggaaaat ccgctcgcca acaccaccgg ccatcagggc tacgtcgcgc cggaaaaggc
      781 tgtcatgcag caggtgaaat catcgctgga acagaaaatt aaacagatgc aaatcagcct
      841 caccggcgag ccggttctcc ggctgaccgg actgtcagcg gcaacactcc cgcacatgga
      901 ttatgaggtg gcaggcacac cggcacagcg caaggtgtgg caggacaaaa tagaccagca
      961 gggagcagag cttaaggcca gaggactgct gtcatgattt actgcccgtc gtgtggacat
     1021 gttgctcaca cccgtcgcgc acatttcatg gacgatggca ccaagataat gattgcacag
     1081 tgccggaata tttattgctc tgcgacattt gaagcgagtg aaagcttttt ctctgactgt
     1141 aaagattcag gaatggaata catttcaggc aaacagagat accgcgattc actgacgtca
     1201 gcctccggca gtatgaaacg cccgaaaaga atgcttgtta ccggatattg ttgtcggaga
     1261 tgtaaaggcc ttgcactgtc aagaacatcg cggcgtctgt ctcaggaagt caccgagcgt
     1321 ttttatgtgt gcacggatcc gggctgtggt ctggtgttta aaacgcttca gaccatcaac
     1381 cgtttcattg tccgcccggt cacaccggac gaactggcag aaagcctgca tgaaaaacag
     1441 gaactgccgc cagtacggtt aaaaacacaa tcatattcgc tgcgtctgga atgagggctg
     1501 ccggttaaca ccggccgtcg ccgcacaccg tatttttatt cttcagcatg atgagaaaga
     1561 gataacgatg gaaagcacag ccttacagca ggcctttgac acctgtcaga ataacaaagc
     1621 agcatggctg caacgcaaaa atgagctggc agcggccgaa caggaatacc tgcggcttct
     1681 gtcaggagaa ggcagaaacg tcagtcgcct ggacgaatta cgcaatatta tcgaagtcag
     1741 aaaatggcag gtgaatcagg ccgccggtcg ttatattcgt tcgcatgaag ccgttcagca
     1801 catcagcatc cgcgaccggc tgaatgattt tatgcagcag cacggcacag cactggcggc
     1861 ggcactggca ccggagctga tgggctacag tgagctgacg gtcattgccc gaaactgtgc
     1921 catacagcgt gccacagatg ccctgcgtga agcccttctg tcctggcttg cgaaggggga
     1981 aaaaattaat tattccgcac aggatagcga cattttaacg accatcggat tcaggcctga
     2041 cgcggcttcg gtggatgaca gccgtgaaaa attcacccct gcgcagaaca tgattttttc
     2101 gcgtaaaagt gcgcaactgg catcacgtca gtctgtgtaa aattccccga aaatccgcct
     2161 gtttttactg aaaaaagcca tgcatcgata aggtgcatgg ctttgcatgc gtttccctgc
     2221 ctcattttct gcagaccgcg ccattcccgg cgcagtctga gcgtgtcagt acaactgcat
     2281 taaaaccgcc ccacaaagcg ggcgggcgag gcggggaaag cactgcgcgc taaaaaggaa
     2341 attgaaatcc agtaacccca aataccttag ctaaaagagc ctaaggaatc taacgcattc
     2401 aactgtcata aaacataatg tgttacataa tggagatagt gtgaaaacaa ctgatataga
     2461 tcatagaagg cttggctccg taagacatct aaggtgttat cttaatcaac tgcagcaaag
     2521 gcattctgcc actgttaatg gaaacttgtt tcgaattgga taacttca
//
LOCUS       DAZFAH010000342         2556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFAH010000342 DAZFAH010000000
VERSION     DAZFAH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.344"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..120
                     /locus_tag="ACHJWB_004833"
     CDS             <1..120
                     /locus_tag="ACHJWB_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4316288.1"
                     /translation="LLLVWIIGRTFEPVIELYTDVTWESFSALHNKINPYKEN"
     gene            122..679
                     /locus_tag="ACHJWB_004834"
     CDS             122..679
                     /locus_tag="ACHJWB_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4316289.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            970..1125
                     /locus_tag="ACHJWB_004835"
     CDS             970..1125
                     /locus_tag="ACHJWB_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4316290.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1245..1589
                     /locus_tag="ACHJWB_004836"
     CDS             1245..1589
                     /locus_tag="ACHJWB_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4316291.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1711..1983
                     /locus_tag="ACHJWB_004837"
     CDS             1711..1983
                     /locus_tag="ACHJWB_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316292.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            1985..>2556
                     /locus_tag="ACHJWB_004838"
     CDS             1985..>2556
                     /locus_tag="ACHJWB_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4316293.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
BASE COUNT          654 a          587 c          703 g          612 t
ORIGIN      
        1 ctattactgg tctggataat tggaaggaca tttgaaccag ttattgagct atataccgat
       61 gtgacatggg aatcattcag cgcactgcac aataaaatta atccgtataa ggaaaactga
      121 tatgagcact attaccagag aacgcgcgga gattaaatca tacatcacag gcttcctgag
      181 cgactcggcg cacgataaca agtcttcaga cagcctgctg gctaatgtgt ttcgtatcgc
      241 gctggcatca ctggaagcag agccgatagc aatggtagtg cctgatgaaa tggatttgct
      301 tacctgccat ctcgacggtg taactaaaac atatgctgat ggctggaacg cctgccgcgt
      361 cgccatgctt caggccggaa actttcggga aaataagaat tcgtcaacca acaattttcg
      421 ggaaatctcg gaaacgtcaa ccagatctcc gataactctg gatggctgga taagctgtac
      481 tgagcgaatg cctgaaaaga gccagaacgt gcttatttcg atgaatatcg atagcgaggc
      541 tgggccatta atatattccg cacgctatct cggaggcacg ttccggcgcg gaggtatagc
      601 agttagtccg ggtaatgatc ttaggcaagc aacccactgg atgtcgctac cagaaccgcc
      661 gcaggaggtg aatcaatgac ctggcctgaa gcattcacaa cggtaggaat tgcgatggcg
      721 gtggcgctgg tggtgtattc gatttgccgc tggggataaa aacggtttgc gggaaaagga
      781 tagttaagta gaattgcagc gggtgcttga ggctatctgc ctcgggcatg aacaccaacg
      841 gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct
      901 gaggctcaat ccatgctgaa cacatgtagg ttagcctctt acgtgccgaa aggcaaggag
      961 aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc
     1021 gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtgcgaat ccgaaccggt
     1081 cagacggagg tcgctgtctt cgtagactac gaatctgaga agtaagagac caggcggggg
     1141 agtaatctcc cgccacctct gatgtgtcag gcatcctcaa cgcacccgcg ctttaccata
     1201 ctgaaaatgc tgtttgaatg ttcatctctg aaagaggact atgaatgaaa aaggtattga
     1261 ttgcagcact tatttccggt gtgtcttttg gcgcttttgc acagcagggt ggtttccagg
     1321 ggccagaagc agagcgttca acagtagcgc aggcaaaaga actgaaggat gatgcatggg
     1381 ttatccttga agggagcatc gttaaaaaag tgggtgatga acgttatgag tttcgtgaca
     1441 atagcgggac aattgtcacg gatattgatg acagcgtatg ggccgggcag aatgtttctc
     1501 cgaaagacaa agtaagaatt gagggtgaaa ttgataaaga cctgagcagt gttgaagttg
     1561 atgtaaaggc actgaaatta ttaaagtaac cgcccctgct tgttaagccc gtcttactga
     1621 cgggttttct gtttgtacat tccggcgtat tgccttacaa ttcgcgcagt cagcctgaac
     1681 aactgacacc tgctgtcacc ggagaatccg atgacacaac acataaaatc ccacaattct
     1741 gaagccgacc cggaaattaa gcaggggagg cgttttcgtg cgcctcagta tggctggttt
     1801 cactatctgt tctgtacgat cgatgaggca gatatgcttc aagaggcgta tctgcgtcgc
     1861 ggtgtccgtg tggagcggag tctgaacgct gatcgtctga cctggaccgt ttctgtatat
     1921 cttcctgttc gtgcacatct gccacggaca catgcctgct accgtcagcg cgtctggagg
     1981 taatgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg tggtccttaa
     2041 gcccggtcgt gaatcattgc cagtttttca tcgcggcagg gtgctggtgg agccggaacc
     2101 gaaaaacatg cgggcgctgc catctggagc ggttcctgct gttcgccagc cgctggcgga
     2161 agataaatca ctgctgccat ttttcagcga tgagcgggtg attcgtgcag ctggcggcgc
     2221 tggtgcactg tctgactggt tattacgtca cgtgaaatcc tgccagtggc cacacggcga
     2281 ttatcatcac agcgaaaccg ttattcacag ttacggtgct ggcgcaatgg tgttgtgctg
     2341 gcactgcgac aaccagctgc gcgaccagac ctccgaatca cttgagcaac ttactcaaca
     2401 aaatctgaca gcctggatga ttgacgtcat acgccatgta atgaatggca cgcaggagcg
     2461 ggaattatcg ctggctgaat tatcctggtg ggcagtctgc aatcaggtgg tggacgcatt
     2521 acctgaggca gtatcgcgtc gctctctggg attacc
//
LOCUS       DAZFAH010000343         2547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFAH010000343 DAZFAH010000000
VERSION     DAZFAH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.345"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..439
                     /locus_tag="ACHJWB_004839"
     CDS             296..439
                     /locus_tag="ACHJWB_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4316294.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            452..661
                     /gene="cspE"
                     /locus_tag="ACHJWB_004840"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             452..661
                     /gene="cspE"
                     /locus_tag="ACHJWB_004840"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4316295.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            827..1636
                     /gene="rlmA"
                     /locus_tag="ACHJWB_004841"
     CDS             827..1636
                     /gene="rlmA"
                     /locus_tag="ACHJWB_004841"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4316296.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1633..2211)
                     /gene="mntP"
                     /locus_tag="ACHJWB_004842"
     CDS             complement(1633..2211)
                     /gene="mntP"
                     /locus_tag="ACHJWB_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4316297.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEDEDEE
                     PRRRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGR
                     FIGSIIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          683 a          603 c          608 g          653 t
ORIGIN      
        1 ttgtatagca aaaaatgtga tattgcacgc actatgtaat aacttctccc actggcctgg
       61 aacaactgaa cttattgaac tatgttagaa aatacgccag tttaagtatc tgcctgaact
      121 ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa
      181 gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat
      241 aattaacgtt gtatgtgata acagatttcg ggttaaacga ggtacagttc tgtttatgtg
      301 tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc gcttctctgc
      361 cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg
      421 ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc aggttaagtg
      481 gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca aagatgtgtt
      541 cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag gtcagaacgt
      601 tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa cagctatctg
      661 atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa
      721 tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt gcccgccgcg
      781 tgattcgcgt tacacttgcg gcctttagta tcctgccgga gttgtcatgt cgttttcctg
      841 tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc cccagcgaca
      901 tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc ataaacggtc
      961 tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct tagatgccgg
     1021 acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc ttgatgaaaa
     1081 ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg catttgccga
     1141 tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga taaaagcggc
     1201 ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt tgccgttttc
     1261 cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag aagaattagc
     1321 acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc gacatttgat
     1381 ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg cagaacaact
     1441 ggaaggtttt acattacagc agagtgatga gttgtgttat ccgatgcgtc ttcgcggtga
     1501 tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc cagaagtctg
     1561 gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata ttcacctctg
     1621 gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga tgccgatcag
     1681 caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa agcgaccaac
     1741 catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg cggtcgcgat
     1801 aatgttgacc tgcaggaaag caagaccaac acccacagcc atggcatcca ggctggtggc
     1861 aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt catcttcatc
     1921 ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc caaggaatat
     1981 cagcagcaca aacgcaatcc agtgattcca ttcaaggaca aaccggctgg ctaacatgcc
     2041 cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg
     2101 caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa
     2161 tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca tgacaatgtc
     2221 ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac
     2281 aagccgcgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc
     2341 tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca
     2401 tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga
     2461 gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac gatttacatt
     2521 tagatttata tatcaatgaa acgttaa
//
LOCUS       DAZFAH010000344         2520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFAH010000344 DAZFAH010000000
VERSION     DAZFAH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.346"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..939
                     /gene="ompT"
                     /locus_tag="ACHJWB_004843"
     CDS             <1..939
                     /gene="ompT"
                     /locus_tag="ACHJWB_004843"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4316298.1"
                     /translation="LGIILTTPIAISSFASTETLSFTPDNINADISLGTLSGKTKERV
                     YLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWM
                     DSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARG
                     GSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSSW
                     VEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKG
                     NTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1126..>2520
                     /locus_tag="ACHJWB_004844"
     CDS             1126..>2520
                     /locus_tag="ACHJWB_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4316299.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLLKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLC"
BASE COUNT          792 a          544 c          523 g          661 t
ORIGIN      
        1 ctgggaataa tcctgacaac ccctattgcg atcagctctt ttgcttctac cgagacttta
       61 tcgtttactc ctgacaacat aaatgcggac attagtcttg gaactctgag cggaaaaaca
      121 aaagagcgtg tttatctagc cgaagaagga ggccgaaaag tcagtcaact cgactggaaa
      181 ttcaataacg ctgcaattat taaaggtgca attaattggg atttgatgcc ccagatatct
      241 atcggggctg ctggctggac aactctcggc agccgaggtg gcaatatggt cgatcaggac
      301 tggatggatt ccagtaaccc cggaacctgg acggatgaaa gtagacaccc tgatacacaa
      361 ctcaattatg ccaacgaatt tgatctgaat atcaaaggct ggctcctcaa cgaacccaat
      421 taccgcctgg gactcatggc cggatatcag gaaagccgtt atagctttac agccagaggt
      481 ggttcctata tctacagttc tgaggaggga ttcagagatg atatcggctc cttcccgaat
      541 ggagaaagag caatcggcta caaacaacgt tttaaaatgc cctacattgg cttgactgga
      601 agttatcgtt atgaagattt tgagctaggt ggcacattta aatacagcag ctgggtggaa
      661 gcatctgata acgatgagca ctatgaccca ggaaaaagaa tcacttatcg cagtaaagtc
      721 aaagaccaaa attactattc tgttgcagtc aatgcaggtt attacgtaac acctaacgca
      781 aaagtttatg ttgaaggcgc atggaatcgg gttacgaata aaaaaggtaa tacttcactt
      841 tatgatcaca atgataacac ttcagactac agcaaaaatg gtgcaggcat agaaaactat
      901 aacttcatca ctactgctgg tcttaagtac accttttaac gaagttaacc agattttctc
      961 cccggtggta ttgtataacc ccggggaatt tgtttacagt tacaagtaga caatatccac
     1021 taattttgta agcatttaac ctatgtcata acgtgtaatc tcttaccgca acacaagacc
     1081 tcaatatatt caaattttat ctttattccg atattcagag acacaatgcg aaaaataatt
     1141 actcatttca aagttgtttt aacgttactt ctaccagtaa ccgtatctgc ccagcagata
     1201 cagtggcaat cctgtatggc cagtcaattc aaccactggt ttggtgagga aaaaccgtct
     1261 cctgacttac tatgtggtta tttgtctgtt ccattaaaat atacagacac aggcggagat
     1321 gcttcttatg aaaaaaaatc acaagtcaaa ctagcgttga caaaattgcc ggcaaaaagc
     1381 aagcataaag gaagtatcct gataataagt ggtggtcccg ggttaccagg cataaatcct
     1441 tatattaact ttgactggcc agtcacaaat cttcgtgagt catgggatat tattggattt
     1501 gatcctcgag gcgtcggaca gtccactccg acaataaact gccggcaatc agatacagag
     1561 actcaggaaa acataaccga aaagcaacaa gtattaaata aaattaatgc ctgtatccat
     1621 aataccggag ccgaagtcat tcgccatatc ggctctaacg aggctgtata cgatattgat
     1681 cgtattaggc aagccttggg ggataaacaa ctgacagccg tggcgtattc gtatggaact
     1741 caaattgcag ccttatatgc agaacgtttt ccctacaacg taagatctat cgttcttgat
     1801 ggagtcgtcg atatcgatga cctggaggac aacttcacat ggcaactcaa acaggcacag
     1861 agttatcagg aaacgtttga tcgctttgca tcctggtgtg cgcgtacaaa aagttgcccg
     1921 ctttcttcag acagagataa ggcaataact cagttccatg agctattatt aaaattacat
     1981 cacaaacctt tattagacag taagggagaa aatatatctt cagatgaact catatcatta
     2041 acaacagacc ttctgctatg gcgttcatca tggccaaccc ttgcaactgc catacgccag
     2101 ttctctcagg ggattgtcag taatgaaatt gaaactgcgc tcagtgctcc gatagcctca
     2161 gaagagtcaa gtgatgcttc gggggtaatc ctttgtgtag atcaggggga tgagcaatta
     2221 acaccagaag agcgaaaatc ccgaaaagac gctcttgcga atgccttccc ggctattaac
     2281 tttgacaatg gacgttccga ttcacctgat ttttgtgaat tatggccaat acatagcgac
     2341 ctgaacaaaa ctcgcctgaa aaatactgtt ctgccctctg gtttactgtt tgtagcacac
     2401 aaatacgacc caacaacgcc ctggattaat gcccgtaaga tggcagagaa attttccagc
     2461 ccgttactaa caataaatgg tgatgggcat acattagctc tcaccggagt taatttatgt
//
LOCUS       DAZFAH010000345         2505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFAH010000345 DAZFAH010000000
VERSION     DAZFAH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.347"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..415
                     /locus_tag="ACHJWB_004845"
     CDS             92..415
                     /locus_tag="ACHJWB_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4316300.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            421..1557
                     /locus_tag="ACHJWB_004846"
     CDS             421..1557
                     /locus_tag="ACHJWB_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4316301.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDDIYAQVAVYSDHFIHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1554..>2505)
                     /locus_tag="ACHJWB_004847"
     CDS             complement(1554..>2505)
                     /locus_tag="ACHJWB_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4316302.1"
                     /translation="RELKIISVIAASENISHAATVLGIAQANVSKYLADFESKVGLKV
                     FDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYL
                     SKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFIT
                     QYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRV
                     KGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFR
                     KREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          619 a          579 c          632 g          675 t
ORIGIN      
        1 atatgccgtt atataaataa ggaaattgca ctgcatcatg ggatttcata atatgcgtta
       61 attgttcttt tttatattct ctgagggaaa tatgtttctg gactattttg cactgggagt
      121 gcttattttt gtatttctgg tgatcttcta tgggatcatt attttacatg atattcccta
      181 cctgattgcc aaaaaacgta atcatcctca tgccgacgcc attcatgttg ctggttgggt
      241 gagtcttttt acgttacatg ttatctggcc gtttttgtgg atttgggcca cgctttaccg
      301 cccggagcgg ggatggggaa tgcaaagcca tgattcatcc gttatgcaac tgcaacagcg
      361 cattgccggg ctggagaaac agctcgccga catcaaatcc tcttctgccg agtaatattt
      421 atggatttac tgattatttt gacctatgtg gcttttgcat gggcaatgtt taagatcttc
      481 aaaattcccg taaataaatg gaccattccc acagcggccc tgggtggaat atttattgtc
      541 agcggtctaa ttctgttaat gaactataac catccgtata cctttaaagc gcaaaaagcg
      601 gttatttcta ttcctgttgt cccacaggtg acaggcgtgg tgatcgaagt gacggataag
      661 aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac tggacccgac gcgttatcag
      721 gcgcgggtgg atcggctgat ggcggatatc gttaccgcag aacataaaca gcgggcgttg
      781 ggcgcagagt tagatgagat ggcggcgaat actcagcagg caaaggccac gcgggataaa
      841 ttcgctaaag agtatcagcg ttacgcacgc ggcagccagg cgaaagtaaa cccgttttca
      901 gaacgcgata ttgatgtggc gcggcaaaat tatctggcgc aggaagcctc cgtaaagtca
      961 tcggcggcgg aacaaaaaca gatccagagc cagctggata gcctggtgtt gggtgaacat
     1021 tctcaaatcg ccagcctgaa agcgcagctc gcggaagcaa aatataacct tgagcagacg
     1081 atagtgcgtg cgccgagcga tggttatgtg acccaggtac tgattcgtcc gggtacctat
     1141 gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac ccgatcagaa acgacaaatt
     1201 gtggcgcagt tccgtcagaa ctccttgctg cgactggccc ctggcgacga tgcggaagtg
     1261 gtgtttaatg ctctgccagg taaggtattc agcggcaagc tggcagccat tagtccagct
     1321 gttcccggcg gagcttatca gtcgaccggc accttacaga cgttaaacac agcgccgggt
     1381 tcagatggcg ttatcgcgac cattgaactg gatgagcaca ctgatttgag cgctttacca
     1441 gacgatattt acgcccaggt ggcggtctac tctgatcatt tcatccatgt ctcggtgatg
     1501 cgcaaagtgc tattacgcat gaccagctgg gtgcattacc tttatctcga tcattaatct
     1561 tcgctttccg ccgcttgttc aggccgcagt tgctgcgact gagcaagctg gtggcgaatt
     1621 tcacgcagca tttcatccag cacatactgg acccgccagg gctggtattc ccgtttacgg
     1681 aagatagcca ccagatccag ccaccattca tacgggtgct gaaagcaggg caccagcgtg
     1741 ccgtcttgca gatcgctctg cacacttttg tctggcgcaa acacaatccc caggtgattt
     1801 cgtgccagct ccagcgccga ttgcgtgtta tcgcaaacgt aatttccctt cacgcgataa
     1861 tcccgcacct ctttactccc ggcgacgttg aagcgccaga tattcgcgtc gtcaatcatc
     1921 atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt caggacggct aataggatgt
     1981 ttctcaagat agcgctggct ggcataagcg gttacggcat attgtgtaat aaaactggca
     2041 accagcgatt catcttttgg tggagcgtag ctgattaaaa catcacaatc atcaggaaat
     2101 tcgacgcctt cataaaaggc attacgctca agattgcagg tttttagcga taaggtgata
     2161 tcaccgatat ctttaatttt atcgattaca tgtttggata aataggtaat tatgccggtt
     2221 ggggcgtaga tggtgacccg accacgtttc tcatgcttat aatctgcaat aaaattatta
     2281 agttgctcgt ttctgtccag catgtcgttg atgtacggta acagcgcggt gccaaaaggt
     2341 gtgagcatca gctgccgggt ggttcggtca aagactttta aacccacttt tgattcaaaa
     2401 tcagcaagat atttgctgac gttggcctgt gcgatgccaa gtacagtcgc ggcatggctg
     2461 atattttcac tggcagcgat taccgagata atttttaact cccgg
//
LOCUS       DAZFAH010000346         2502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFAH010000346 DAZFAH010000000
VERSION     DAZFAH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.348"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /gene="escO"
                     /locus_tag="ACHJWB_004848"
     CDS             complement(<1..209)
                     /gene="escO"
                     /locus_tag="ACHJWB_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO4316303.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLS"
     gene            complement(269..1609)
                     /gene="escN"
                     /locus_tag="ACHJWB_004849"
     CDS             complement(269..1609)
                     /gene="escN"
                     /locus_tag="ACHJWB_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO4316304.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMETNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1593..>2502)
                     /gene="escV"
                     /locus_tag="ACHJWB_004850"
     CDS             complement(1593..>2502)
                     /gene="escV"
                     /locus_tag="ACHJWB_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4316305.1"
                     /translation="RWNFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLIDD
                     TVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTNQQLAHLTGMDVYATTNDKITFLLKK
                     LVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENVSIRD
                     LRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVSGTLLNVWLIGSDIENELR
                     ESIRQTSSGSYLNISPERTEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMIEGSFPS
                     VPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          721 a          593 c          456 g          732 t
ORIGIN      
        1 ttcgaaagtg aagatttttt ctcatacaat cgctgctttt gctcatcaaa tgcattgttt
       61 ttaatctgat atttatacaa cgacacttta tccaaattca ccagcgatgc acaatatgcc
      121 tgcttagagg caatcgactc tttaatggat tgctcgcagt catcgagttt accatccacc
      181 tcttttatct gagagttaat cttagccatt gattctctca atcggttcgc tctgctttta
      241 cgaatagata aaattctgtc caacatactc aggcaaccac tttaaatagg ctttcaatcg
      301 ttttttcgta actactgata tctttggtgc tttgttgtat aaagttctga attaatttcc
      361 tattttttat tgccttatct gcttcaggat cttgccccat cgtgtactca ccaatacgaa
      421 taagcagctc aacattttta taagttgcaa taagcttttt gcattctgcc gccgcacgca
      481 aatgctccga tgtaacaacg ttatgcataa cacgactggc acttaaacca atatcaattg
      541 cagggaaatg gttttcctct gcaagttctc gggtaagtac gatgtgccca tcaagaatag
      601 agcggacttc atcgccaata ggatcattaa cattatcgct ttctaacaac accgtataaa
      661 tcgcggttat tgaacctttt ggcgcaggcc ccgctcgctc taataatttt ggtaacgaag
      721 aaaaaacact cggagggaat ccccccctta catcaggttc tcccgatgca agcccaacat
      781 cacgtgcggc tcgggcataa cgagttacag aatccatcat taataataca tttttacctt
      841 gatcgcggaa gtactctgca atagtcgtcg ccgtaaatgc ggctttcatc ctttccagcg
      901 cggggcggtc tgacgttgtg acgaccagta cacatttaga aagcgtggat tgaggtaaga
      961 gcgcgaggaa ttcatttact tcgcgaccac gttcgccgat aagagcaagg acaataatat
     1021 ctgcggatgc gccattacaa atcatcccca aaagcgtact tttaccaacg cctgaaccag
     1081 caaaaatacc gatacgctgc ccaataccgc aagttagtag cccatcaatt gccctgacgc
     1141 caagtataaa tggctgatca ataacctgcc ttaataaagg atctggtggt tcagcataca
     1201 agctgcgttc aaacggaaga tagggggcag caatattagt ctccattggt ctgcctatgc
     1261 catcaacaag ccgcccaagt agtgcgtctc caacacgaat cttgaactca tctccctgat
     1321 aacttaacca ctgaccacag tacatgccgg aaacatgctc aaaaggcaac aagaaaacct
     1381 catcttcatc aatagcgatc acctcggcta aacgttgact gggttctatc ttataaaagg
     1441 cgccaatacg cgcttttggt aaacgcgcct taataatcgt cccgccgata ttgataatct
     1501 tgccttcata tctggtagac gaatttaatg ataatgccgg cacagtgcta ttgagcactt
     1561 tctgaatacg tgggtatttt tccaatacag aatcatgctc tgaaatcatt taccgttcct
     1621 aatactttaa gttcgatatt attcccaacc tcctgaaaag agagcacggg gacagacggg
     1681 aacgaacctt caatcatttt cttcacatag cgcctgatat ctaaagcggt cagaatgacg
     1741 ccgttccccg ttggattcat gatatttttt aagaagccaa ttatctgctc agttcgctcc
     1801 ggtgagatat tcagatacga acctgatgac gtttgtctga tcgactctcg tagctcattt
     1861 tcaatatcag agccaataag ccaaacgttc agaagtgtac cgctaacgct atagcgacct
     1921 aaaatatgcc gacgcagggc gatacgaacg tattcgcaca agataaccac atccttttct
     1981 tttgttgacc aaaaaataag cgtctcaaag atagttctca ggtctctaat tgagacattt
     2041 tcctctacga gacgttgtag gatgtcaaca attttgctca aaccaagctg gcgctgcagc
     2101 tctttcacaa gctcgttata ttttctctcc atgatgtcca tcaaataacg cgtttcttgt
     2161 acgccgatga actctttggc attagacaat acaagtttct ttagcaaaaa cgttatctta
     2221 tcatttgttg ttgcataaac atccatacct gtaaggtgag cgagctgttg attagtctta
     2281 ttcgtcttat atacgataga gttagtcgaa agcttttcct ggacaaattc gaatgatatc
     2341 tctgcatgcc cagcctcaaa atagacagta tcatctataa gagtatcttt gtatattgac
     2401 tcttgataga gcaaaaattc tattgcgcta tcattatttt taaccggatt aacaataatc
     2461 ttaggcaatg gaattccact ttcctcaaag aaattccaac gc
//
LOCUS       DAZFAH010000347         2483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFAH010000347 DAZFAH010000000
VERSION     DAZFAH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.349"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..158)
                     /locus_tag="ACHJWB_004851"
     CDS             complement(<1..158)
                     /locus_tag="ACHJWB_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4316306.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALAR"
     gene            complement(142..384)
                     /locus_tag="ACHJWB_004852"
     CDS             complement(142..384)
                     /locus_tag="ACHJWB_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4316307.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            776..1114
                     /locus_tag="ACHJWB_004853"
     CDS             776..1114
                     /locus_tag="ACHJWB_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4316308.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1407..1562
                     /gene="ydfA"
                     /locus_tag="ACHJWB_004854"
     CDS             1407..1562
                     /gene="ydfA"
                     /locus_tag="ACHJWB_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4316309.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            1564..1692
                     /locus_tag="ACHJWB_004855"
     CDS             1564..1692
                     /locus_tag="ACHJWB_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316310.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLSSSAKSNG"
     gene            1722..1940
                     /gene="ydfC"
                     /locus_tag="ACHJWB_004856"
     CDS             1722..1940
                     /gene="ydfC"
                     /locus_tag="ACHJWB_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4316311.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSVLEQDITNLEKAAALSIARMITYPR"
BASE COUNT          698 a          575 c          588 g          622 t
ORIGIN      
        1 cgagccaggg cttccggatg gctgtcgtca tacagttctg ggaacgtcat acccaactca
       61 aaataagcct gggttattcc agctgctgga actttttcgc catcaggacg cgcccaggca
      121 ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcccccctt ggttagaagg
      181 cggattatga tcagaaccgg gaatgacaac cgtcggtatg tgtaactcat atttgagcgc
      241 cccggcagtg actgcctgaa ttagcaacgc ccatttccac ggaacctctt ccccccacat
      301 actgactgtg gtttttgacg ttcctagagc tgcggctgtt ttaacaactc cgccaaaata
      361 gcctaatact tctgattttt tcatgagacg ctccataaaa ctgaacgtca aaagtttaat
      421 aatcaaaacc aaagaaagtc aagaaacaaa accatctgtg ttttaaaatc aaaacatgag
      481 caagcaaact atatctgaac gcataaccca acgtatgcat gcgctaaacc tgaaaggaaa
      541 agaccttgtc aatggcactg gcgcatcaaa aggctccgta agtcaatgga tgaacggtgg
      601 aggagcgccg tcctcgcgtt acataagttc actggcaaaa atattgaaag taaacgaaaa
      661 ttggcttctt aatggaggag agttaaatac aggtgattcg cttgatctat ctttaccgcc
      721 gataaaaacg gttccgctac tatcacttca gcaggcagca agctggagtg attatatgaa
      781 aaattcctca ataacctctt gtgtgcagct tgtcggagaa atcccggcca atacctttgc
      841 agttgttcta gagagtgaca gtatgtcaac atctggtggg ggagtttcca tcccaaatgg
      901 ttcaacagtt tttgttgatc ccgatcgaac cgtacaacca ggaaatattg tccttgcctt
      961 acccaaaggg accacaacac ctgtcattcg taaactggag atagaagggc cggatattct
     1021 tttagtcccc acgaatcctc gctacccttc aattatgctg gatgatctat cttgcatatt
     1081 gggcgtatgc tttaaaattc aacaagatat ttaaccaacc tcatctattt gattaactgt
     1141 atgccatcgt ggtgatggct taacagctgc ctgcttaaaa tgttttgata aaaaaacatt
     1201 gacctaaaaa gttcgttttt ctaaacctta ttcattccct caccccatcc tacagaatgc
     1261 agggcaatac ttcgagttac caggcagtgg tcaggggtta agtagccagc ccgaggcgta
     1321 agaacatgac ggcagggttc aactttaata actatgcagc aggtttttgt tccgctaccc
     1381 cggcgttaag gggaaatgag gtcagcatgg atactatcga tcttggcaac agcgaatctc
     1441 tggtatgtgg cgtgttcccc aaccaggacg gtacgttcac cgcgatgacg tataccaaaa
     1501 gcaaaacgtt taaaaccgaa aatggtgccc gtcgctggct ggaaagaaac tcaggtgagt
     1561 gatatggatt ttgacacaat catggaaaag gcttacgaag aatacttcga aggccttgcc
     1621 gaaggcgaag aagctcacag cttcaacgaa tttaaacagg tgctttccag ttcggcaaaa
     1681 tctaacggct gataagcgaa acagcaccgc gaggaatcag tatgcagaaa cgagaacccg
     1741 tcatcatcgc gccagactat accgatgatg aactttatga gtggatgcgc cagaaaatta
     1801 atgcagcgca ggatctgaaa tgggctaatg aagccagggc taagcaggct gaaaatctgt
     1861 ccgttctgga gcaggatatc accaatctgg aaaaagcagc ggcattaagc attgccagaa
     1921 tgattacata cccgcgttaa tagctaacca acgaagctaa ggttggtaat taaggagttc
     1981 tccacgggtg aggtggagtg cgtgcgccgg acacgggtga gcatccggca ctgacagttt
     2041 actgaaagga tatttccctg aaaagtcaga ccataacgcg aaagcgcacg gcgaggtagc
     2101 tggttcatag atagcctgtc gttaaatttt cgtcgaccgt gcgcttccgg ttgtggcaat
     2161 ccgcgaaatg gcgcggcggt aagtatggcg gggttattcc ttcccccgtt gaggacaccg
     2221 ggttgtcagg ttgaccatac gcttaagtga caaccccgct gcaacgccct ctgttatcaa
     2281 ttttctggtg acgtttggcg gtatcagttt tactccgtga ctgctctgcc gcccttttta
     2341 aagtgaattt tgtgatgcgg tgaatgcggc tatgcgcacg cggaacagtt aaaaccgtaa
     2401 agtggtattt tacggggcgc aacggacatc cttctgtatc cggcgttaat tgttaactgg
     2461 ttaacgtcac ctggaggcac cag
//
LOCUS       DAZFAH010000348         2460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFAH010000348 DAZFAH010000000
VERSION     DAZFAH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.350"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2460
                     /locus_tag="ACHJWB_004857"
     CDS             <1..>2460
                     /locus_tag="ACHJWB_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4316312.1"
                     /translation="PEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNA
                     GFMDRLCAGTSLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDTALCDKENKPPRI
                     AQGSSNVFINNRPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMIL
                     GGITIASYLLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIA
                     NKVIRWVTDPVDPVTGAYCDERTDFTQGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWS
                     EYAWVREQGNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALS
                     SRFFYDAFPGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRI
                     TGVSHTDGIRLKLMYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFY
                     EYDAADRIIRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGI
                     HRYYYDPDGNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYT
                     AADGAEWQYRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGL
                     LSGIFRQDERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLRW
                     QQHHKLSGLERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHR
                     WQYRYDKDTLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARV
                     NGLEETILYSRDAAGRLAEVIS"
BASE COUNT          558 a          703 c          776 g          423 t
ORIGIN      
        1 acctgaagtc ccacaggggg aaccctcatg gggtgatatt gccactgacc tgcttgaatc
       61 ggcagcggaa aaagcagtac cactggcgaa ggcctggggg aacgctgtta tcaccctgac
      121 ggacagcaat gccggtttta tggatcgctt atgtgcaggc acatcgcttc tgtttcccgc
      181 cggtccggta ttaatggagt ttgccaccat ggtgggcggg cgtggcgaaa tcaaaaaaga
      241 ggtggatttc ccggaagccg gtgaggacac ggcgctctgc gacaaggaga acaaaccacc
      301 gaggatagcc cagggcagca gcaacgtctt catcaacaat cggcctgccg cacgccaggg
      361 cgacaaactg gagtgcagcg cagcaatcgt gggaggttcg ccggacgtct ttattggcgg
      421 tgagcaggtg acctatctgg atatccagcc ggagttcccg ccgtggcaga gaatgatcct
      481 ggggggaata acgatagcca gctatcttct gccgccagcg ggactgctgg gaaaactgaa
      541 gaatctggcg agactcggca aactggggaa cctgctgggg aaaagcggga agctgctggg
      601 ggcaaagctc ggcgcgttgc tggggaaaac aggtaagtcg ttaaaaagta ttgccaataa
      661 agtcatcaga tgggtaacag atcctgtcga tccggtaacc ggcgcgtact gcgacgaacg
      721 taccgacttc acccagggcc agacactccc cctctccttc acccgtttcc acagttctgt
      781 actgccgctg catggcctga ccggcgtggg ctggagcgac tcctggagcg aatacgcctg
      841 ggtgcgtgaa cagggaaatc gggtagatat tatcagccag ggagccacgc tgaacttcgc
      901 cttcgacggt gacagtgata cggcggttaa cccgtatcac gcccagtaca ttctgcgccg
      961 ccgcgacgat tacctggagc tgttcgacag ggatgcactg agcagccgct tcttttatga
     1021 cgcctttccg ggaatgcgtc tgcgccaccc ggtgactgac gataccagcg atgaccgcct
     1081 ggcacacagc cccgacgacc ggatgtatat gctgggcggg atgagcgaca ccgccagcaa
     1141 ccgcatcacg tttgagcgcg acagccagta ccgcatcacg ggtgtcagtc acaccgacgg
     1201 gatccggctt aaactgatgt accacgccag cggctacctg aaagccattc accgcacgga
     1261 taacggcata cagacgctgg cgacctacga acaggatgcg cgggggcggc tgacagaagc
     1321 ggatgcgcgg ctggactacc acctgtttta tgagtacgac gctgcggacc ggatcatccg
     1381 ctggtccgat aacgaccaga cgtggagccg tttcacctac gatgaacagg gccggtgcgt
     1441 gaatgtcacc ggggcggagg gctattacaa cgccacgctg gactatggtg acggctgcac
     1501 caccgtgacg gacggcaagg gcattcaccg ttattactat gatcctgacg gcaatattct
     1561 gcgggaagaa gcgccggacg gcagcaccac cacgtatgaa tgggatgaat tccatcacct
     1621 gctggcccgc cactcccctg ccgggcgggt ggagaagttt gaatacaacg ccgcacacgg
     1681 tcagttaagc cgttacacgg cggcagacgg cgcggagtgg cagtaccgct atgatgagcg
     1741 cggcctgctc agcaacatca ccgacccagc cgggcagacg tggacacagc agtgtgatga
     1801 acgcggcctg ccggtaagtc tggtgtcgcc acagggcgaa gagacccggc tggcgtacac
     1861 gccgcagggg ctgctgtcgg ggatattccg ccaggatgaa cggcgtctgg gcatagagta
     1921 cgaccaccac aaccggccgg aaacactcac cgacgtgatg ggccgtgaac accataccga
     1981 atacagcggt cacgacctgc cggtgaagat gcgcggcccc ggcggtcagt cagtacggtt
     2041 acggtggcag cagcaccata aactgagcgg ccttgaacgt gctggaaccg gcgcggaagg
     2101 atttcgctat gaccgccacg gcaacctgct ggcctggacg gacggcaacg gcgttgtctg
     2161 gacgatggaa tacggcccgt tcgatttgcc ggtggcgcga acagacggtg aaggccaccg
     2221 ctggcagtac cgctacgata aagacacgct gcaactgaca gaagtcatca atccgcaggg
     2281 cgagtcatac cgttatattc tggacaactg tggacgggtg acggaagagc gtgactgggg
     2341 cggcgtggtc tggcgttacc gctatgacgc cgatggcctg tgtaccgcca gggtcaacgg
     2401 cctggaggaa accatcctct acagccggga cgccgcaggc cgcctggcag aagtcatctc
//
LOCUS       DAZFAH010000349         2451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFAH010000349 DAZFAH010000000
VERSION     DAZFAH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.351"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(55..450)
                     /locus_tag="ACHJWB_004858"
     CDS             complement(55..450)
                     /locus_tag="ACHJWB_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4316313.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(447..1022)
                     /locus_tag="ACHJWB_004859"
     CDS             complement(447..1022)
                     /locus_tag="ACHJWB_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316314.1"
                     /translation="MAIKGLEQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPGELAYALKQQLRIAIKR"
     gene            complement(1038..1391)
                     /locus_tag="ACHJWB_004860"
     CDS             complement(1038..1391)
                     /locus_tag="ACHJWB_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4316315.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGGEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1384..1758)
                     /gene="gpFI"
                     /locus_tag="ACHJWB_004861"
     CDS             complement(1384..1758)
                     /gene="gpFI"
                     /locus_tag="ACHJWB_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4316316.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1810..>2451)
                     /locus_tag="ACHJWB_004862"
     CDS             complement(1810..>2451)
                     /locus_tag="ACHJWB_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4316317.1"
                     /translation="QAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGKEWSKQ
                     DRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQLETA
                     VKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIRCYGAI
                     QDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          563 a          767 c          542 g          579 t
ORIGIN      
        1 gtggtaccgg ctccttttac cggcgccagg ggatttggtg ttgccatatc gtcctcacat
       61 ctcgtatgta atggaataag tcagatctgc agaactccat aacgccatat cgtcatcacg
      121 acgatactca tagccctgcg taaccatcgt ggtaatcagt cctgccagtg ccgggatcgc
      181 ggtcatcgcc ggataaatcc ggctttccat ccacgagtcc agctctgaat ccggtacctg
      241 tgccggtaaa aacacctcaa tatgcagtgt ggcccgccag gtatctgcat ccagctcttc
      301 accggtatac tctgcatccg tcagataaac cgcgatcgca ggaaaatcct cttcgtcaaa
      361 aacaacgggg cgaccatcaa acagcgtcgc cccgtgttca tgctgctcga gtgcatccag
      421 cactgccgca cggatatcag tatgtttcat cgttttatcg caatcctcag ctgttgtttc
      481 agcgcgtatg ccagttctcc gggcagacgt tcacgccgga tacggtcaac gttttcatca
      541 aacgcctgtt tcagtggggc cgccatcggg attttcacca cctgaatagg aaggcgatta
      601 cgctttttcc tgcccttgtc gtcattgccc ttctcatatc tggcctgggg aagacgttgc
      661 ataacatgcc agcgcccatt atttaatcgc tggataaatg cccgctgata acgatgctga
      721 ccggctttga gtatgctgtt cggacgacgc cccagcattc tgatccccag cttaatcaca
      781 ggaagatcac cgcggttaac gataattttt gcattcggat ttctgaccgt ggcccgtttc
      841 agtctggacc gttccttaac cagtttccgt ctcaccctgg tttcccgggc aacctgtgat
      901 gaagactgat taatcgccgt tgtggccacg cggttaatgg tcattgcaga agccgccgga
      961 atggcgtttt tacgaacccg gctcagatta tcaatcgcct gctcaagccc ttttatcgcc
     1021 ataatttcac cctgcgttta tcgtcgccgg ttaacggcgg gtggttgccc acggttgagc
     1081 cagagataac agctgccccc gtcatccgga gaaacacgat ccacccagaa cgtctcacca
     1141 ttaatggtca gcgtgtcacc acgccgcaca gcacgaaccg tatccgtccg cacaaataat
     1201 gacgggctgc ttccttcaat acggaccccg ctaccggcaa atcccagcga ctccggatcg
     1261 tcaaaaaccc cctgaacttc tccgccacac tgtgcccccg aggtgaactg tgcacagagc
     1321 cccatcactt caacaatcgt gctgtccacc ccggcgaggg cagcatcaaa ggcattctga
     1381 aaatcacgca taaacagcca ttccgccatc aacgtgtgtt tttgcatctg aggacataat
     1441 cagaatcacc cgaccaacat ccgcaagctc aacggattcc cctgtttcac catcaatgcc
     1501 acagagatgg aggcaggtca gaactctgat gcgcgttaac gcaccggatg tatcctcacg
     1561 aacatcatga gccgcggttt cccgctcccg gatcaccgta tccccaacct gaacatcctc
     1621 gccggatgac tgtatttcct cttcccattc cgccacccgc tgcgctatct ctgcggcact
     1681 cccggatata tccggctcac gccccagaat cagggccagt tcatcaagcc gtttcagatt
     1741 ttgctctttc gttgccatat cagccccctg tgaaaaaaga cacgggggca tttcgccccc
     1801 gctcacggat tatttcacct gtaccaccac aaactcatcc gggtccggca acaccatcag
     1861 cggcgcggac tgcgtcatgg taaattcacg ggcgggatcc cccaccgtca gccagtgttt
     1921 cggataacgg gaagaggcca ccacaccttc ggacaacgcc tgcgcatcct gaatggcacc
     1981 gtaacaacgg atcccatctg cagcagtatt ccccagaacc agcatgccct ccggcagata
     2041 acgtttttcg ataccgtctt ctgctatata agacgttttc gccaccacaa tggccagatc
     2101 gccgtaatac cccttgaagg acaccactgc gcccagatct ttcactgccg tttcgagttg
     2161 agaatttgaa ccgcgacggg tatccagttt ttcgcggaac agcttaaaac cattcagaag
     2221 acgccagacg gtaccgtcca taatggcaat attcacaaga ccgctggcct ggtcgcagta
     2281 gaggtcaaga tcatgtgtag gatcgaacgt gtcacgatcc tgttttgacc actccttacc
     2341 actaccctga gtgatgttat tcttcgtcga cctgccaaaa tcgacctcaa ttttctcgaa
     2401 ctggtctcct tccatcgtat atttgccata caacacagca tttaccgcct g
//
LOCUS       DAZFAH010000350         2445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFAH010000350 DAZFAH010000000
VERSION     DAZFAH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.352"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            73..1197
                     /gene="gmd"
                     /locus_tag="ACHJWB_004863"
     CDS             73..1197
                     /gene="gmd"
                     /locus_tag="ACHJWB_004863"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4316318.1"
                     /translation="MKNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTER
                     VDHIYQDRHNENPDFILHYGDLTDSSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEY
                     TADVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAV
                     AKMYAYWITINYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLY
                     LGNMDSLRDWGHAKDYVRMQWMMLQQERPEDFVIATGKQISVREFVRMAAKEVGLELQ
                     FSGKGIDEIATVVNKTSDCAIGVNVGDVIVRVDPRYFRPAEVETLLGDPSKAKKLLGW
                     EPEITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            1201..2166
                     /locus_tag="ACHJWB_004864"
     CDS             1201..2166
                     /locus_tag="ACHJWB_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFO4316319.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSQRDDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLMGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDFTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIGNINSFRG"
     gene            2169..>2445
                     /locus_tag="ACHJWB_004865"
     CDS             2169..>2445
                     /locus_tag="ACHJWB_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFO4316320.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITLEELGVQISLNDALFLGVYEHFYN"
BASE COUNT          730 a          434 c          542 g          739 t
ORIGIN      
        1 ttcgaaaatt tggagaaacg gtggtttcag aaatttattt taaattgata gattatgtta
       61 gaggagatga atatgaaaaa taaagtagct ctaattactg gtgttaccgg tcaagatggt
      121 tcttacttgg cagaatttct tcttgaaaag ggttatgaag tgcacggtat aaaacgccga
      181 gcatcttctt ttaatactga acgtgtagat catatctatc aggatcgtca taatgaaaac
      241 ccagatttca tacttcatta tggtgactta acggactcat caaacctgat acgcttgatt
      301 aaagaaatcc aaccagatga agtttataac cttggagcac aatctcatgt agcagtttca
      361 tttgaatcac ctgaatatac tgctgatgta gatgctatgg ggactttacg tctgctggaa
      421 gcgatccgta tttgtggact agagaaaaag acgcgtttct atcaggcatc aacttctgaa
      481 ctcttcggct tagtccaaga aattccgcag cgagaaacta caccgttcta tcctcgttcg
      541 ccttatgctg ttgctaagat gtatgcatac tggattacta taaactatcg tgaatcttat
      601 ggtatgtatg cctgtaacgg tatcttgttc aaccacgaat ctccacgtcg tggcgaaaca
      661 tttgttacac gtaaaattac tcgtgcaatt gctaatattt cgcaaggaat cgaaaaatgt
      721 ctctatcttg gtaacatgga ctcattgcgt gactggggac atgctaaaga ttatgtccgt
      781 atgcagtgga tgatgcttca acaagaacgc ccggaagatt ttgtaatcgc taccggtaaa
      841 caaatttctg tacgtgaatt tgtccgaatg gcggctaaag aagttggctt ggaacttcag
      901 ttctctggta aaggtattga tgaaatagct actgttgtaa acaaaacatc tgactgtgct
      961 attggcgtaa atgttgggga tgtaattgtt cgtgttgatc cacgctattt ccgccctgcg
     1021 gaagttgaaa cacttcttgg tgatccaagt aaagctaaaa aattattggg ttgggaacca
     1081 gaaattactg ttgaagaaat gtgtgcggaa atggttgcca gtgatctggc gaaagcaaaa
     1141 cagcatgcac tactgaaaag ccatggttac gatgttgcag tttctctgga gcggtaaggt
     1201 atgacaaaaa aacgtatcta cgttgctggt caccgaggta tggttggctc tgctatttgc
     1261 cgtcaattat cacagcgtga tgatatcgaa ttagtggtca aaacacacaa agaactcgat
     1321 ctaaccgtac agaaggatgt tgatgcattt tttgagcaag agaaaattga tcaggtttat
     1381 cttgctgcgg ctaaagttgg tggcatttat gccaacaata catttccggc agaattcatc
     1441 tatcagaatc tcatgattga gagtaatatt attcattcag ctcacaaggc cggaattcaa
     1501 aaattacttt ttttaggctc aagctgtatt tatcctaaat tcgcagagca gccgatgaac
     1561 gagtcagcgc ttttaatggg catacttgag ccaactaatg aaccatacgc aattgctaaa
     1621 attgcgggca taaaattatg tgaatcttat aaccgtcaat atggccgtga ttatcgcagt
     1681 gtaatgccta ctaatcttta tggcataaat gataatttcc atcctgaaaa ctctcatgtt
     1741 attccggcac tcatgcgtag attccatgaa gcaaaagaga gtggtgcgcc agaggttatt
     1801 gtctggggaa ccggaacacc gatgcgtgag tttttatatg ttgatgatat ggctgccgca
     1861 tccgtccatg taatggagct tgacgaagca atttatcaac aaaatacaca gcctatgtta
     1921 tcccatatta atgttggtac gggtgtagat tgttctatac gtgaaatggc tgaaacaatg
     1981 gcctctgtgg tgggttatca aggtaaaatt gtttttgatt ttactaaacc tgatggcact
     2041 ccgcgtaaac ttatggacgt tacccggctc aaaaacttgg gctggcaata tcgctataat
     2101 ttacatgaag gcttatcatt aacttataaa tggtttattg ggaatattaa ttcttttcgg
     2161 ggatagttat gaacaagaga ttggaacgtg agctatttaa aacaatagtt gagcatactc
     2221 ctctaatctc gattgatctc ataattagaa acgataaagg agaggcgctg ctggggcagc
     2281 gcctgaatcg cccagcacaa aattattggt ttgtgccagg agggcgaatt tataaggatg
     2341 agtcattcga gattgcattt aagcggataa cacttgaaga gttgggcgtt caaattagtc
     2401 ttaatgatgc cttatttctt ggggtgtatg aacattttta caatg
//
LOCUS       DAZFAH010000351         2444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFAH010000351 DAZFAH010000000
VERSION     DAZFAH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.353"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..583
                     /gene="eco"
                     /locus_tag="ACHJWB_004866"
     CDS             74..583
                     /gene="eco"
                     /locus_tag="ACHJWB_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4316321.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(732..2378)
                     /gene="mqo"
                     /locus_tag="ACHJWB_004867"
     CDS             complement(732..2378)
                     /gene="mqo"
                     /locus_tag="ACHJWB_004867"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4316322.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          580 a          670 c          608 g          586 t
ORIGIN      
        1 ttattcataa agcaaattaa taaatctgat gaatatgtta accttcagcg acatcatcgg
       61 tgaaaaccta taaatgaaga aggaaagcaa aaaaatgaag accattctac ctgcagtatt
      121 gtttgccgct ttcgctacca cttccgcctg ggcggcagaa agcgtccagc cactggaaaa
      181 aatcgcgcct tatccacaag ctgaaaaagg gatgaagcgt caggtgattc agttaacccc
      241 gcaagaagat gaatctaccc tgaaagtaga actgttaatc ggtcagacgc tggaagtcga
      301 ttgcaatttg catcgtctcg gcgggaagct ggaaaacaaa acgctggaag gctggggcta
      361 tgattattat gtctttgata aagttagctc gccggtttcc acgatgatgg cctgcccgga
      421 tggcaagaaa gagaagaaat ttgtcaccgc gtatctgggc gatgctggaa tgctgcgtta
      481 caacagcaag ctgccgatcg tggtgtatac gccagacaat gtagatgtga agtaccgcgt
      541 ctggaaggcg gaagagaaaa ttgacaacgc ggtagttcgc taaaatgctg tgaagtgcgg
      601 caccccgtag gccgaataag gcggtcacgc cgcatccgac atctaacgcc cgagccggtg
      661 cctgatgcga cgctggcgcg tcttatcagc atacgccaca tccggcatac catgccggat
      721 gtggcacatc attacaacgc aatatccgcc acttcttttt gaacgggttg cggtttcaac
      781 tgcggttttg gcgtactatc tgctgcctgc ggcttgtcgt atttcagccc cagcacttcg
      841 ctggtgtact gcaactcgcg ttctgttgcc gctacatcac cgttcagctt gcgtccataa
      901 gacggaacga tcgctttcaa cgtagcctgc cattgcgggc tggaaacgcg atcgccaaat
      961 actttttcca gcagatccaa cataatcggc gcggcggttg acgcccctgg cgatgccccc
     1021 agcagcgcgg caatggtccc ttgctggtca ctgaccactt cagtacccag acgcagtacg
     1081 ccacctttct cggcatcacg cttgataatc tgcacgcgct gccccgcttg ccacaaacgc
     1141 cagtcctctt tttttgcctg cggatagtac tctttcaacg cttcaaaacg atcctcttca
     1201 ctcaacatca cctgactcac cagatatttc accagatcga aattatccag cccgacgtgc
     1261 atcatcggca tcacgttaga ggtggtggtg gaactcatca gatcccacaa tgaaccgttt
     1321 ttgaggaatt tggttgagaa ggtggcaaat ggcccaaaca gcactacgcg tttaccgtcc
     1381 agaacgcggg tatcgatatg cggaaccgac atcggtggtg cgccaacgga tgctttaccg
     1441 taaaccttcg ccagatggtg attaaccacg tccgggtttt ccgaaacaag gaactgtccg
     1501 cccaccggga aaccggcgta gtctttcgct tccggaatcc ccgattcctg taatagcttc
     1561 agcgccgcac cgcccgcgcc gataaagaca aatttggcgc ggatgttctg tgcagtgccg
     1621 tttttcagat cggcaacggt aacggtccag gtgttatcgt cattacgctt tagggcgcgg
     1681 acttcgctgc tgagttgcag cgagaagtta gatttcttct gtaaggaagc aattaactgg
     1741 cgggtgatct cgccatagtt cacatcagtg ccaatttctg tgcgtgttgc cgctactttc
     1801 tgttgtggat cgcgcccttc catcactaac ggtgcccact ctttgatctg cgcgtgatct
     1861 tcagagtaac gcataccgcg aaacagcgag ctttgttgca acgcggcgta acgggcgcgc
     1921 aggaaattaa cgttatcctc gccccagaca aagctcatat gcggaacggt attgataaat
     1981 gaacgcggag tacgcagcac gccgcgctca acctggtgcg cccagaactg gcgggaaatc
     2041 tgaaatgctt cgttaatggc gactgctttt tcaatactga tgctgccatc ggcgttttgc
     2101 ggggtgtagt tcagttccat cagtgcagaa tgcccggttc cggcgttatt ccagccgttc
     2161 gaactctcct gcgcgacacc ctccaggcgc tccaccatgg tcatcgacca ttcaggctcc
     2221 agctcgcgta aataggtccc caacgtggcg ctcataatgc cgccgccaat caacagtaca
     2281 tcagtttcct gctcctcgga cgctttcgct tttgccgcca tcgaaacggc attaagcccc
     2341 acggccatcg agaagagcat ggcagtcact tttttcatct tgttaatgcc ttacttttag
     2401 tcgctttatt gcaggtgaga tttgcgcggc atcaacggta acac
//
LOCUS       DAZFAH010000352         2442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFAH010000352 DAZFAH010000000
VERSION     DAZFAH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.354"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..2308
                     /gene="adiA"
                     /locus_tag="ACHJWB_004868"
     CDS             41..2308
                     /gene="adiA"
                     /locus_tag="ACHJWB_004868"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4316323.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPDDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          614 a          672 c          602 g          554 t
ORIGIN      
        1 tgcccgcaac gaagattcct tcataaccgg gtaagcaatg atgaaagtat taattgttga
       61 aagcgagttt ctccatcaag acacctgggt cggtaacgcc gttgagcgtc tggcagatgc
      121 tttaagccag caaaatgtta ccgtgattaa atccacctcc tttgatgatg gttttgccat
      181 tctctcttca aacgaagcca ttgactgcct gatgttcagc tatcaaatgg aacatccgga
      241 cgaacatcaa aacgtcagac aattgatcgg taagcttcat gagcgccaac aaaacgtgcc
      301 ggtcttcctg ttgggcgatc gggaaaaagc cctcgccgca atggatcgcg acctgctgga
      361 gcttgtcgat gaattcgcct ggattctgga agataccgcc gactttatcg ccggacgcgc
      421 cgttgccgcg atgacccgct accgccagca gctgttgccg ccactgttca gcgcgctgat
      481 gaaatatagt gacatccatg aatattcctg ggcagcgcca ggccaccagg gcggcgttgg
      541 ttttaccaaa acacccgccg gacgtttcta ccatgactac tatggtgaaa atctgttccg
      601 caccgacatg ggcatcgaac gaacttccct cggttctttg cttgaccata ctggcgcatt
      661 tggcgaaagc gaaaaatatg ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt
      721 cgtcggtact tccggctcta accgcaccat catgcaggct tgcatgaccg ataacgatgt
      781 cgtggtcgtt gaccgtaatt gccataaatc catcgaacaa ggcttgatgc tgacaggcgc
      841 gaaaccagtc tacatggtgc caagccgcaa ccgctacggc attatcgggc caatctatcc
      901 gcaggaaatg caacctgaaa ccttgcagaa gaaaatcagt gaaagcccgc tgaccaaaga
      961 caaagccggg caaaaaccgt cttactgcgt ggtgaccaac tgcacctatg acggcgtgtg
     1021 ttataacgct aaagaagcgc aggatctgct ggaaaaaacc tccgatcgtc tgcactttga
     1081 cgaagcctgg tacggctatg cacgtttcaa cccgatctat gccgatcact atgccatgcg
     1141 cggcgaacct gacgatcaca acggtcctac cgttttcgcc acccactcca cccacaaact
     1201 gctgaatgcg ctttcacagg cttcttatat tcatgtacgt gaaggtcgtg gggcgattaa
     1261 cttctcccgc ttcaaccagg cctacatgat gcatgccacc acctccccgc tgtatgccat
     1321 ctgcgcatcc aacgacgtgg cggtgtcgat gatggacggc aacagcggcc tgtcactgac
     1381 gcaggaagtg atcgacgaag cggttgattt tcgtcaggcg atggcgcggc tgtataaaga
     1441 gttcaccgct gacggtagct ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc
     1501 acaaaccggc aaaacctatg actttgctga cgcaccaaca aaactgctga ccaccgttca
     1561 ggactgctgg gtaatgcatc cgggcgaaag ctggcacggc ttcaaagata ttccggataa
     1621 ctggagtatg ctcgacccaa ttaaagtcag catccttgct ccgggaatgg gtgaagatgg
     1681 tgaactggaa gaaaccggtg ttccggcggc gctggtcact gcctggcttg gtcgccacgg
     1741 cattgtgcct actcgcacca ctgacttcca aattatgttc ctgttctcta tgggcgtaac
     1801 ccgtgggaaa tgggggactc tggttaacac cctttgctcc ttcaaacgcc actatgacgc
     1861 caacacaccg ctggcgcagg tgatgccgga acttgttgaa caatatcctg acacttacgc
     1921 gaacatgggg attcacgatc tgggtgacac catgtttgcc tggctgaaag aaaacaaccc
     1981 tggcgcacgg ttgaacgaag cctattccgg cctgccggtg gcggaaatca ccccgcgtga
     2041 agcgtacaac gcgattgtcg acaacaatgt cgaactggta tccattgaaa atctgccagg
     2101 acgcatcgcg gcaaactcag ttatcccgta tccgccagga atcccgatgc tgctgtctgg
     2161 tgaaaacttc ggcgataaaa acagtccgca agtaagttat ttacgctcgc tgcaatcctg
     2221 ggaccaccat ttccctggat ttgaacacga aactgaaggg actgaaatta ttgacggtat
     2281 ttaccacgtt atgtgcgtga aagcgtaacc actattccgc tgaaggcgta attgtttaaa
     2341 taacattacg ccgcctggcc ttaggccttt tgagtatggc aacgttttca taaaaattgc
     2401 tgcaaacaaa aatgtcatac tttttgcgcg gccccacccc gc
//
LOCUS       DAZFAH010000353         2384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFAH010000353 DAZFAH010000000
VERSION     DAZFAH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.356"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHJWB_004869"
     CDS             <1..99
                     /locus_tag="ACHJWB_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316324.1"
                     /translation="LNYVNNRERSLVKQILIEDEIIVCATYLIPDI"
     gene            101..445
                     /gene="ymgG"
                     /locus_tag="ACHJWB_004870"
     CDS             101..445
                     /gene="ymgG"
                     /locus_tag="ACHJWB_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO4316325.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            449..784
                     /gene="ymgD"
                     /locus_tag="ACHJWB_004871"
     CDS             449..784
                     /gene="ymgD"
                     /locus_tag="ACHJWB_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO4316326.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRELKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(841..>2384)
                     /locus_tag="ACHJWB_004872"
     CDS             complement(841..>2384)
                     /locus_tag="ACHJWB_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4316327.1"
                     /translation="TNSKITGSANISTDDNTHTYLSLSDNSTWDIKADSTVSNLTVDN
                     STVYISRADGRDVEPTRLTITENYVGNNGVLHLRTELGDDNSATDKVVINGNTSGTTR
                     VKVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIFAGAYEYSLTRGNTEATNKNWY
                     LTNFQATSGGETNSGGSSAPTVAPTPVLRLEAGSYVANLAAANTLFVMRLNDRAGEMR
                     YIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSDSW
                     RLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYATWFADDISKKGAYIDAWAQYS
                     WFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIWMG
                     VDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGSHGDDFEPFVEMNWIHNS
                     KDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVG
                     LKYKF"
BASE COUNT          607 a          567 c          565 g          645 t
ORIGIN      
        1 ttgaattatg tgaataatag agagcgttct ttagttaagc agatcttaat tgaagacgaa
       61 attattgtgt gtgcaactta cttaataccg gacatctaag ttgaagaaaa aaatacttgc
      121 cttcgggctt atctcagcat tgttctgctc cacgccagcg atggcggata tgaatcgcac
      181 cacgaaaggt gcattgctag gcgcaggcgt tggtttactt actggcaatg gtgttaatgg
      241 cgtactgaaa ggtgcagctg ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga
      301 cggtaaaaat gcgcgtaaag gtgcaaaagt gggtgccgcc gttggtgctg tcactggcgt
      361 tctgaccggc aatggcctcg aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg
      421 tgcaattctg ggtaaaatga aataaggaat gatgatgaaa aaatttgctt tactggcggg
      481 cctgtttgtt ttcgcaccaa tgacctgggc gcaggactac aatattaaaa acggtttgcc
      541 gtcagaaacc tatattactt gcgcagaagc caacgaaatg gcaaaaactg acagcgcaca
      601 ggttgcagaa attgttgcgg taatgggtaa tgccagcgtt gccagccgtg aattaaaaat
      661 tgagcaatct ccggaactga gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa
      721 agacccgcaa atgctgttga tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa
      781 ataatatttc atatgtaccg cgtcgatgaa tacgacgcgg ttttttagct aacggcggga
      841 tcagaactta tatttcaggc ccaccatcac tgcggtgtca ttgtagccat tatcacccag
      901 ctgcacgccc acattccccc agacgctggc cgctggattc aggttgccat ttacgccaag
      961 tttaatttcc cccaaattac tcgccccatc ttgttcgact ttcacaccgt tcattgagac
     1021 agcaaaatct ttactgttat ggatccagtt catttcaaca aaaggttcaa agtcgtcacc
     1081 gtgcgaaccg ctgtttttct cctgagtacg aataaaggtg cggaaaccaa gtcgggtctg
     1141 gatgttgtta tttgcgtcat tctcaatacg tgagccattg gcttcggtgt gcgcattatg
     1201 atcgacgccc atccagatag cctgtgcctg tggctggaat atccacgtgt atttcgccgc
     1261 ttccagacca aaggatttat tcagggcaaa gccgtaaccc gcttccagcg agacggttgc
     1321 gcctttcgca ctataggatt cataggctaa ttcatcccct ttcaccgagt ttttaaacca
     1381 gctatattgc gcccaggcgt caatgtatgc gcctttttta ctgatgtcat cggcaaacca
     1441 ggtggcatac agccctgcgc aatagcctct gacactccct ttcgaacgat aatccgacac
     1501 gctggaatga gttgagttgt agtcgcgggc ataaccagcc atcactccca aacgccaact
     1561 gtcactgtcg gtaaaaccac cggttaacag atcggcccct aactgcgaga cgtagcgatg
     1621 cgaggtcgtt ctcaactgtc cgttgctgtc acgccaggca ttatgcccgc caatttgacg
     1681 tagccaaagt cggcttgaac gctcctgttc agttacagga tcgatgtagc gcatttcacc
     1741 cgcacggtcg ttcagacgca taacaaaaag agtgttagcg gctgccaggt tggcgacgta
     1801 acttccagct tcgaggcgca ggacgggggt aggcgcaaca gtaggcgctg aacttcctcc
     1861 ggagtttgtt tcaccgccgc tcgttgcctg gaagttagtc agataccagt ttttattggt
     1921 cgcttcggta ttacctcggg ttaatgaata ttcgtaggca ccggcgaaaa tcctcgaatc
     1981 cttaataaat tccccatttg attccccctc aacgctgata atctctatcc catttaacgt
     2041 gtaagctccg ctgcctcctg cattggtaac tttaactcgg gttgttccag aggtatttcc
     2101 attaataacg actttatccg tagctgaatt atcatcaccc aattcagttc tgagatgcaa
     2161 tacgccatta ttaccaacgt aattttcagt tattgttaat cttgttggtt cgacatcccg
     2221 tccatctgcc cgggaaatat agaccgtact attatcaacc gtgaggttgc tcaccgttga
     2281 gtcagcttta atatcccagg tactattatc tgaaagcgac agataggtat gagtgttatc
     2341 atctgttgaa atattagctg aacccgttat ttttgaatta gttg
//
LOCUS       DAZFAH010000354         2323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFAH010000354 DAZFAH010000000
VERSION     DAZFAH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.357"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..680
                     /locus_tag="ACHJWB_004873"
     CDS             <1..680
                     /locus_tag="ACHJWB_004873"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4316328.1"
                     /translation="PPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVG
                     RAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRG
                     LIVLTDRYPQDQIPGSYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLN
                     VDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLIDAEKAITDF
                     MTARGYH"
     gene            complement(734..2233)
                     /gene="pitB"
                     /locus_tag="ACHJWB_004874"
     CDS             complement(734..2233)
                     /gene="pitB"
                     /locus_tag="ACHJWB_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO4316329.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          617 a          601 c          565 g          540 t
ORIGIN      
        1 cacctccatt aattgccgtt attggtagcg atggttcagg caagtcaacg gtgtgtgaac
       61 atcttattac cgttgtcgaa aaatatggtg ctgccgaaag agttcattta ggaaaacaag
      121 ccggaaatgt cggtcgtgca gtgacaaaat taccgttgat gggaaaatcc ttacataaaa
      181 caattgaacg aaatcaggtg aaaacagcaa aaaaattgcc aggaccagtt ccggcgctgg
      241 taattacagc gtttgtcgcc cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc
      301 gtcgcgggtt aattgttcta accgaccgtt atcctcagga ccaaattcct ggctcttacg
      361 atggtacggt gttcccacct aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc
      421 aggaacgtaa agcgtttcac tggatggcga gccataagcc tgatctggtc atcaaactca
      481 atgttgacct tgaagttgcc tgcgcgcgta aacccgacca taaacgggaa tcgctggcga
      541 ggaagattgc cataacgcca cagttaacct ttggtggtgc acaactggtt gatatcgatg
      601 ccaatcagcc actggaacag gtgctgattg atgcagaaaa agcgattacg gattttatga
      661 ccgcgcgtgg ttatcactag tcaaaaatgg aactgcccga tccgatcgcc aggaccgggc
      721 attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa
      781 ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta
      841 acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg
      901 agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg
      961 ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta
     1021 cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc
     1081 acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct
     1141 ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca
     1201 gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat
     1261 tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt
     1321 gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa
     1381 tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtaacggcat
     1441 cgcgggtacg ggtaatttca tagccggacg cattcatatt gacgacgaag ccagcagggg
     1501 caatccccac cagtaccagc attaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt
     1561 gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt
     1621 tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt
     1681 tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga
     1741 cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat
     1801 ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa
     1861 tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa
     1921 tcgccgccag cagcatggaa aagaccatcg ccagaccgtg ggttgacccc atattcagca
     1981 acaaatcggt tggtaacata tggacaatgg cataggcaac gctaagtccg cccaataaca
     2041 cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag
     2101 tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga
     2161 acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa
     2221 ataaatttag catatattag attacgccat tttgaattta cgaacggacg cattatcagt
     2281 gactttaacg gcatgggcaa agtgaaatat cattttttaa cga
//
LOCUS       DAZFAH010000355         2307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFAH010000355 DAZFAH010000000
VERSION     DAZFAH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.358"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /locus_tag="ACHJWB_004875"
     CDS             complement(<1..79)
                     /locus_tag="ACHJWB_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4316330.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVA"
     gene            complement(161..982)
                     /locus_tag="ACHJWB_004876"
     CDS             complement(161..982)
                     /locus_tag="ACHJWB_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4316331.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGFVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(1203..1613)
                     /locus_tag="ACHJWB_004877"
     CDS             complement(1203..1613)
                     /locus_tag="ACHJWB_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316332.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(1629..>2307)
                     /locus_tag="ACHJWB_004878"
     CDS             complement(1629..>2307)
                     /locus_tag="ACHJWB_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316333.1"
                     /translation="LPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGKLP
                     EGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWA
                     EQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTV
                     WFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT          558 a          566 c          554 g          629 t
ORIGIN      
        1 tggcaaccaa ctgaggcgga ttattctctg gtgttgtagc tgaataaatt gtgggggtat
       61 tctgagaaac ggttttcatg agataactcc ttaaataaca aataaatgtg tgtttttcag
      121 gaacgaaagc tgaatgattc cctcactcag ggaaacggca tcagcgcagg ctctccagca
      181 tcgtttctgc catcacccac aatgcgcgat tgagcttaat gtcggtatca atgctgtgaa
      241 tggcccgggt atggatacgt tttccttttg cactgcgacc ggaaatcccg cctttcagca
      301 tattctcctg gatggtctga tatgcgctcc acaggtcctt accgtaatcc tcccggcgtc
      361 gcggcgtcag aatgtcggcg gtggtgacgg gctgatgttc gtcaccataa cggtaagtca
      421 gtgccgcctg tgccagcgcc tggcgtgccg gtggcggcag gaccagcgac tgcatggcat
      481 cacgcttctc ctctatccgg tcaaacacgc ccaccacctc gtaagcccct tcgataactt
      541 tctccactac atttccccgg tgtggaacac gcacttcccc cagagactgg ccacagacga
      601 acccgttctg gcagacgaac ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt
      661 catgagagtt gagcagaata atttcaggga catgttctcc gtttatctct ccggcccgcc
      721 gcagacgcag catgtgtttt gtgtatcccc ggcggcccgg gtcgcgcaca cgggtctggc
      781 aggcgaagaa cggctgaaag ccttcccgct gcagattttc caggacggta atggtgggaa
      841 tgtacgcata ccgttcactg cgggaggtgt gccggtcttc cccaaaaata ctgggtacat
      901 ggcgtatcag ttcttcatgt gtcagcggac ggtcacggcg tatctggttt gcagcataac
      961 caaaacgact tgctaatcgc atactttgct ccttatcggt ggttaagatt tactggtgca
     1021 ataaatgaaa aaagccgcgt ctcccggaga agacgcggcc tgacgggtga aatgaataag
     1081 gtttattgtc tgagaggcct ttcactggcg agccacgcat taagcggctt tccggtaaca
     1141 atggctgggg ctgtatttac ctttgatatt aataatgtgc agagaaacgc atggtttact
     1201 ccttagattt taagtttgtc ctgggctaca acgacaggtt ttccggatgt gtcgatgact
     1261 cctaccgtcg gtacgccatt tttgatttcg aagaacggtt tgttgctacc tgagcacagt
     1321 tgattgtccc cgtattcaat catttgtgct ttcccggttt tcaggttaac taccgagaca
     1381 gtgtagtcgt agcaccaggc accaccacgt tcaagcacga aaacatagct gttatcttta
     1441 tagttgaact gattctttgt ctggcaattc ataccgtcaa tttcggtaca ggaaaacgct
     1501 tctgtagtat taacgtattt gatgttattt ggcagtttta cttgtttcgc agtagcgaag
     1561 acagagaaca gaatagttac agcaacgagt aaagttttga taaaagtttt catgatagac
     1621 tccttaaatt agttaaccgc agctacgtag tttttcacca gatacgcgaa ttcatgcacg
     1681 ccaatttcac cgaaccaaac caggtaggcc gggtcatcta aaccaaagta acggtagcca
     1741 acaacttcat gggtttttgg gttatagagt gttacgataa agtcccagcc gcaggtattg
     1801 gctttgcaac cgtcgccctg gaaccagact gtaccgttaa tgtttactgg cgtgatacct
     1861 ccaccagggc catccatata gccgccttta ccgaacatgc cgctcagtcc gaccggtttt
     1921 aatgctttgt cgatagcggc tttgtaggta gcgtcacctt cgtacaactc gtacgttgat
     1981 ggacacgctc caggttgttc agcccaggct tccgggttac aactaagagc ttgttttacg
     2041 gtagccgctt ctgcgttaaa gttaaagccc gtgaacagaa ccgccagaaa taacataagg
     2101 gtatgtaaac gtttcattgt aaatattctt ccgttgtaaa tggcgctgga aaatttctca
     2161 tcagtgaaac cttccggaag tttccccccc ttgtatgatg tatatcgcca gtattttatt
     2221 tcatcccggc tgtaattttt atattccccg gcacgtgcaa taacgctgac cagagatata
     2281 aggctcatta ataactttac aggtaat
//
LOCUS       DAZFAH010000356         2305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFAH010000356 DAZFAH010000000
VERSION     DAZFAH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.359"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..630
                     /locus_tag="ACHJWB_004879"
     CDS             127..630
                     /locus_tag="ACHJWB_004879"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4316334.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(676..1092)
                     /gene="rraB"
                     /locus_tag="ACHJWB_004880"
     CDS             complement(676..1092)
                     /gene="rraB"
                     /locus_tag="ACHJWB_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4316335.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1254..2258
                     /gene="argF"
                     /locus_tag="ACHJWB_004881"
     CDS             1254..2258
                     /gene="argF"
                     /locus_tag="ACHJWB_004881"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4316336.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          594 a          546 c          580 g          585 t
ORIGIN      
        1 acgttacaat actcttatca gtaataggct ggcaaaaact ttattacttt ataagtcgct
       61 tgcgcacgta agaagagagt ggtattaaac aaatgtataa ttactttatt tttattgggt
      121 taaactatga ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gcagcaggat
      181 aatcccgcca tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat
      241 aaaggctaca ccgtcgccga tccgaacctg gacgagttgt atcaagtgta tagtcagccg
      301 ggtcatgcat attgggtggt tgagtacgat ggtgaagtgg tcggcggcgg tgggattgcg
      361 ccattaaccg ggagtgagtc ggacatttgc gaactgcaga agatgtattt tcttccggct
      421 attcgcggta aagggctggc gaaaaaactg gccttaatgg cgatggagca ggcacgagag
      481 atgggcttca aacgctgcta tctggaaacg accgcttttt tgaaagaagc gattggtttg
      541 tatgaacatt taggttttca acatatcgac tatgcacttg gctgcacggg ccatgtagat
      601 tgtgaagtgc ggatgctacg cgaactctaa aaaaatgccc gctagtgtgt gctatcgggc
      661 attttaatca attaattagt ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc
      721 gtcgccgtct tcgccattgg gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc
      781 aaatttctcc gccagcgtca tcagttgttc aacctgggca tcgatcagat cggcattcaa
      841 cgcgcactcg ctgaggatgt cgcagcaaat cacgatatca ccatcttcaa cttccagctc
      901 ttctggatcg gtcacttcgt aaccgagttt aaacgcttca actgctgctt tttccagggt
      961 ttccagatcg tctgcggaaa gatggtgttc gatggtgtac agcgcgtccg gatcgctgcc
     1021 atcttccagt aattcttcaa taatcaaacg tgtttcttca cgctgttctt ccagttgttc
     1081 cgggtttgcc atggctcatt cctcttaatg tcctgccgat actcttattg tcacacacca
     1141 ctgacattgc ctccaccttt gtgacaaaga tttatgcttt agacttgcaa atgaataatc
     1201 atccatataa attgaatttt aattcattaa ggcgttagcc acaggaggga tctatgtccg
     1261 ggttttatca taagcatttc ctgaaattac tcgatttcac gccagctgaa ctcaacagcc
     1321 tgctgcagtt agccgcgaag ctgaaagccg ataagaaaag cggcaaagaa gaagccaaac
     1381 tcactggtaa aaacatcgcg ctcatcttcg aaaaagactc gacccgtacc cgatgctctt
     1441 tcgaagttgc cgcatatgac cagggtgctc gcgttactta tctcggccca agcggcagtc
     1501 agattggtca taaagagtcg attaaagaca ctgcccgcgt gctgggtcgc atgtatgacg
     1561 gtattcagta tcgcggctat ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg
     1621 tgccggtatg gaacggcctg accaatgagt ttcaccccac gcagttgctg gcggatctcc
     1681 tcaccatgca ggagcattta cccggtaaag cattcaacga aatgacgctg gtctatgcag
     1741 gtgacgcacg taacaacatg ggcaattcga tgctcgaagc tgcggcgctt accggtctgg
     1801 atttgcgtct ggtcgcgcca caggcatgct ggccggaagc tgcgctggtt acggaatgcc
     1861 gcgccctggc acagcaaaat ggcgggaata ttacgctgac ggaagatgta gccaaaggag
     1921 ttgaaggtgc tgactttatc tataccgatg tatgggtgtc gatgggggaa gcaaaagaga
     1981 aatgggcaga acggattgca ttactgcgtg attatcaggt gaacagcaag atgatgcagt
     2041 tgaccggtaa cccggaggtc aaattcctcc actgcctgcc cgcgtttcat gacgaccaga
     2101 cgacgcttgg taagaaaatg gcggaagagt ttggcctaca tggcggaatg gaagtgactg
     2161 atgaggtctt cgaatctgcc gccagcattg tttttgatca ggcggaaaac cgcatgcata
     2221 ccatcaaagc ggtgatggtc gcgacgctca gtaaataatt gatatcgcta taaagatgcc
     2281 gggaaataca aaaacatccc ggcat
//
LOCUS       DAZFAH010000357         2292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFAH010000357 DAZFAH010000000
VERSION     DAZFAH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.360"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..665)
                     /locus_tag="ACHJWB_004882"
     CDS             complement(<1..665)
                     /locus_tag="ACHJWB_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4316337.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCDCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGRQTDRLVDLERMLHLFSGKPIPDNR
                     ENIT"
     gene            complement(750..947)
                     /locus_tag="ACHJWB_004883"
     CDS             complement(750..947)
                     /locus_tag="ACHJWB_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4316338.1"
                     /translation="MKSLTTETELDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(959..1447)
                     /locus_tag="ACHJWB_004884"
     CDS             complement(959..1447)
                     /locus_tag="ACHJWB_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4316339.1"
                     /translation="MKLALTLEADSINAQTLNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(1444..1820)
                     /gene="cbtA"
                     /locus_tag="ACHJWB_004885"
                     /pseudo
     CDS             complement(1444..1820)
                     /gene="cbtA"
                     /locus_tag="ACHJWB_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(1910..2275)
                     /locus_tag="ACHJWB_004886"
     CDS             complement(1910..2275)
                     /locus_tag="ACHJWB_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4316340.1"
                     /translation="MSDTLPGTTHPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLA
                     DRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEADT
                     LGSCGYVYLAVYPTPETKK"
BASE COUNT          512 a          568 c          624 g          588 t
ORIGIN      
        1 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
       61 aggtcgacga gtcggtcagt ctgtcgcccg gtgataagat gaaaccccca ccggtcccac
      121 ctcaccaggt tgttgacgat aattttactg ccaaatttgc aggggcaatt ggttttgtaa
      181 ttccagttca gtcccctgaa gacgttgatc acccctcgct caaacacctc atccttattc
      241 tggtgtagtt gttcaaatgt gctcagaatg ttcgcttcac tgatttccgg aaaattatcg
      301 tactccagtg accggtacca tgtatcacgg gcctgtgcat ccattaagga aagcatccct
      361 gaacgttgca tcaggtcacg ccagatttcg cgatcgagat tgcgtgtgat ggctttcatt
      421 gcggtttccg gtttctccat cagccagcag tcgcagcgaa aatcctgttt catggcccag
      481 tccggggcgg ctttaccgcc aatactttct gtcattgctg atatttcagc cagttgccgg
      541 ataagtgtgt cgatttgcag tagtgtactc tttcgtacag ccagaatgtg gcggatactg
      601 gttgagcaga taacgtccgt atgcccggtg accacttccg gacgggagat tgtgatgtct
      661 gacattatcg gtttcccttt tggtttataa acgaaaacgc cagccctgtt caggctgacg
      721 tcagtggaat gaagtatggt gagtgatatt taccggttct ggtgcagaag ctgcagatga
      781 cgaagagtac ggacatcctc ccgggcctgt tcgatacagg gcaacagtgc tgccgaatcc
      841 gttttatcct catcgttgtc aaagataatt cccgattcgc agtcgatatt gtcctgcagc
      901 cacgcaatga ggatatccag ctctgtttca gtggttaatg atttcatgtt gtttctggtt
      961 accagttgaa agtaggtaaa cccggcaggc attcagcgct ggcatccaga tgaatgagac
     1021 tgacgccata acgccggata agtgtgacaa ccagacggcg gaacattttt gacagcccca
     1081 ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt acgtaacaga tatccgctac
     1141 ctgtaaaatg aatccattct gattcgccga agtcactggt ctggtgtgac agcgagtaca
     1201 accaggcgtt gtctgtttcc gtgatatgtg cggtgctgca gcgtatgccg gtaagggttg
     1261 ttaacggtgg tggtgtgcag gttgactgtt ggtcggagtc gtccaccaca cggagtgaat
     1321 aaccgttttc agcgaccttg ttaatcagtt cagtgagatt aacaccatcg acgtcaacga
     1381 caatgcgccc catgttcagc gtctgcgcat taatgctgtc ggcttccagc gtcagggcca
     1441 gtttcattgt ttcgcctccg gatacttacc cagggtaatg ttatttaccg ttctgtaatt
     1501 gtcgcgggtc atcaggccgg tcgccctgcg agccaggagg atatcgatgc tgtttattaa
     1561 ctgagagcgg gtacaggcgc tgaatcccgg ctggtcggta gcaccagcgc gtatttttcc
     1621 acgagaaagt tcaccgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca
     1681 cgttcatcgg caaacggtgt gtcattcagt gtgaggccat agtgctggtc cagcagtcgg
     1741 gacagcagta tctgccagat ttcaacagga gacgggcgag aactagccgc ctgcccgggt
     1801 aatacaggta atgttttcat actgaagatt ttcctgatat gcagagataa aaaacgggac
     1861 agtggcgtgg tgaaaacacc aggccggagc agagggttat tccggtggtt tactttttcg
     1921 tttcgggcgt cggataaaca gccagataaa cgtaaccaca ggagcccagg gtatcggctt
     1981 cgcaggtcag tcctttcgca tacagcgtga cggtatgctg atagcggggg ctgagttcac
     2041 cactggtgag catgagctcc agttgtttca tcagcagcgg aaaggcctgg tccaggtggt
     2101 acgaatccgc gtcgctgaac cggcctctga taccggcgcg gtctgcaagg taatgcaacc
     2161 ggttaccctc ctgcaccaga cgtgccccga aacagggcat cacggtgcag ggtagccccc
     2221 accaggggcg gttgttgttg tcgggatgcg ttgtcccggg cagtgtgtct gacacgataa
     2281 agtcctcaca ga
//
LOCUS       DAZFAH010000358         2281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFAH010000358 DAZFAH010000000
VERSION     DAZFAH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.361"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..300)
                     /gene="fumD"
                     /locus_tag="ACHJWB_004887"
     CDS             complement(91..300)
                     /gene="fumD"
                     /locus_tag="ACHJWB_004887"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4316341.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            655..849
                     /locus_tag="ACHJWB_004888"
     CDS             655..849
                     /locus_tag="ACHJWB_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316342.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            857..2269
                     /gene="pykF"
                     /locus_tag="ACHJWB_004889"
     CDS             857..2269
                     /gene="pykF"
                     /locus_tag="ACHJWB_004889"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4316343.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          627 a          563 c          497 g          594 t
ORIGIN      
        1 taatttcagc agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca
       61 ggatgaagct taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacagtttc
      121 catcgcctgc ttttccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa
      181 tacgcctgcg ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac
      241 tttaccgaca actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat
      301 tctttatacc tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa
      361 aatcaaacaa aatcagacaa ataacgcgat aaattatttt aactgtagca attgagcgat
      421 gatatattta tacaccggat gaactttcac ttatcctcac actgacaact tcggcaccag
      481 acgttgcgca aacagtgaag tttttgcgta accttttccc tggaacgtta aatctttgat
      541 aacaatttat tgtctaacaa gttgtatatt ttttgaaacg ctgtttttgt tttccttttg
      601 gattaatttc agcgtataat gcgcgccaat tgactcttga atggtttcag cactttggac
      661 tgtagaactc aacgactcaa aaacaggcac tcacgttggg ctgagacaca agcacacatt
      721 cctctgcacg ctttttcgat gtcacctatc cttagagcga ggcaccacca ctttcgtaat
      781 accggattcg ctttccggca gtgcgcccag aaagcaagtt tctcccatcc ttctcaactt
      841 aaagactaag actgtcatga aaaagaccaa aattgtttgc accatcggac cgaaaaccga
      901 atctgaagag atgttagcta aaatgctgga cgctggcatg aacgttatgc gtctgaactt
      961 ctctcatggt gactatgcag aacacggtca gcgcattcag aatctgcgca acgtgatgag
     1021 caaaactggt aaaaccgccg ctatcctgct tgataccaaa ggtccggaaa tccgcaccat
     1081 gaaactggaa ggcggtaacg acgtttctct gaaagctggt cagaccttta ctttcaccac
     1141 tgataaatct gttatcggca acagcgaaat ggttgcggta acgtatgaag gtttcactac
     1201 tgacctgtct gttggcaaca ccgtactggt tgacgatggt ctgatcggta tggaagttac
     1261 cgccattgaa ggtaacaaag ttatctgtaa agtactgaac aacggcgacc tgggcgaaaa
     1321 caaaggtgtg aacctgcctg gcgtttccat tgctctgcca gcactggctg aaaaagacaa
     1381 acaggacctg atctttggtt gcgaacaagg cgtagacttt gttgctgctt cctttattcg
     1441 taagcgttct gacgttatcg aaatccgtga gcacctgaaa gcgcacggcg gcgaaaacat
     1501 ccacatcatc tccaaaatcg aaaaccagga aggcctcaac aacttcgacg aaatcctcga
     1561 agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt gtagaaatcc cggtagaaga
     1621 agttatcttc gcccagaaga tgatgatcga aaaatgtatc cgtgcacgta aagtcgttat
     1681 cactgcgacc cagatgctgg attccatgat caaaaaccca cgcccgactc gcgcagaagc
     1741 cggtgacgtt gcaaacgcca tcctcgacgg tactgacgca gtgatgctgt ctggtgaatc
     1801 cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg gcgaccatct gcgaacgtac
     1861 cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac aaccgtaaac tgcgcattac
     1921 cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa ctggatgctc cgctaatcgt
     1981 ggttgctacc cagggcggta aatctgctcg cgcagtacgt aaatacttcc cggatgccac
     2041 catcctggca ctgaccacca acgaaaaaac ggctcatcag ttggtactga gcaaaggcgt
     2101 tgtgccgcag ctggttaaag agatcacttc tactgatgat ttctaccgtc tgggtaaaga
     2161 actggctctg cagagcggtc tggcacacaa aggtgacgtt gtagttatgg tttctggtgc
     2221 actggtaccg agcggcacta ctaacaccgc atctgttcac gtcctgtaat attgcttttg
     2281 t
//
LOCUS       DAZFAH010000359         2274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFAH010000359 DAZFAH010000000
VERSION     DAZFAH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.362"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(511..945)
                     /gene="uspF"
                     /locus_tag="ACHJWB_004890"
     CDS             complement(511..945)
                     /gene="uspF"
                     /locus_tag="ACHJWB_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4316344.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1086..2219)
                     /gene="ompN"
                     /locus_tag="ACHJWB_004891"
     CDS             complement(1086..2219)
                     /gene="ompN"
                     /locus_tag="ACHJWB_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO4316345.1"
                     /translation="MKSKVLTLLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GQVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          616 a          490 c          529 g          639 t
ORIGIN      
        1 cgtgccgcgg ttggctggta agcttttcga tagtgcgagt atctaatgat ttccagccgg
       61 aactgattct attggaaaga aatcatgggc gcaatttaca gatcacagat tgattagtta
      121 tagattttta ctgtggcaaa aatgggacaa aaggccgcaa aaggggcaaa aatggggcaa
      181 aaaaagtgta gattattgtg ctttattgta gttgttaatc ctgcctaaca tattgaaaaa
      241 cataaaagtt attgttcgtc agatagttgc ggtttttact ctgacttgtt caggttgtat
      301 tgttctttct tactaatttc ttgattttgc gacatttaaa agcgactcaa ttcgttatat
      361 ggcatcagaa gagtatgcgt catgccggaa cgcccagcat aagaaatctg atataaaaac
      421 tgtggcgtgt atggtacgga ttagagggga aaatgtcagc acatttgcga aatgaatcaa
      481 aaagcccgca gcaatgtgcg ggcgttagtg tcagcgcaca accagcacgg agcactctgc
      541 gtgacgcact acagctgcgg cgttggaacc gagcagataa gtggtgatat ccggtcgatg
      601 ggaagcaatg atgatcatat gagcggggat cttcttcgcc aattccagaa tgcggtcttt
      661 gggcgagcct tcctcaacat ggacatgcac tctgtcggtt ggcagtttaa attttttaat
      721 gatctcttcc agttgcgatt tggcttccgc tttcaggtca tccattgccg gtaattctgc
      781 ggaatacgct aaacccagag aggcatagta gggcagtgaa ggtattaccg tcaggaaatg
      841 aacctctgca tcatcaatct ttgcctctgc ctcaacgtgg ctaatcacgc gttgagttaa
      901 ttctgaatcg gaaatatcga tagggacaag aatcgttctg ttcataaaac ctcctgtttt
      961 agtatccgca taaagtgtaa cgccagatga cactttttgt gtaatgacgg agttcacatt
     1021 tttaatttag atcaaaggag gaagaataag cagaaaaagc ccgccataac agcgggcagg
     1081 aggatttaga actgataaac cagacctaaa gcgacaatat catcggtaga gatgccgttg
     1141 gcagcgtaga aactgtcatc ttcatccaac aggttgattt tatagtcaac ataggtggac
     1201 atgtttttat tgaaatagta agtcgcgcca acatcggcgt atttaaccag atctttatca
     1261 tcaacacctg ccgggttgtc tgcaccaccg gcagcgtgca ggtcacggcc tttagacatc
     1321 aggaaagaga ctgccggacg cagaccaaaa tcaaactggt actgtgcagt gacttcaaag
     1381 ttctgggttt tgttcgcaac tgcataatcg ctgtcgccaa acggggtcat attacgcgtt
     1441 tctgaataca tggttgccag gtaaatattg ttagcatcgt attttagccc agcagtccac
     1501 gcgtctgctt tatcaccacc cgccgcagta tggttaacct ggtcattggt gcggtcagaa
     1561 gaggtgtatg ccgcaccagc gctaaagccc atgcctaaat catatgttgt ggaaagaccc
     1621 cagccgtcac cgttttcatg gcgaacatca cgtccgttgt tggtgccttc ctgaccatta
     1681 ctggcacctt cgttgttacc ttgatactgc accgcgaagt tcagaccatt tacctgaccg
     1741 aagaaatcag tattacgata agtcgcgacg ccgttggcac gaccagtcat aaagttgtct
     1801 gcattggtat aagagtcacc gccaaattca ggcagcatat cggtccagcc ttcgatgtcg
     1861 tacattacgc cataattacg tccgtaatcg aaagaaccgt aatctgcaaa tttcagtccg
     1921 gcaaatgcca gacgggtcca tgactggttt tttgaagatt cagtgttgtt tgcctgaata
     1981 ttgtattccc attgaccgta gccagtgagt tgatcgttaa tttgggtttc gcctttaaaa
     2041 cccagacgcg catagctctg gtcgccatct ttcgctgaat tatcagaaaa ataatgcagg
     2101 ccatcaactt tgccatacag atctaatttg ttgccgtctt tattataaac ttcggctgca
     2161 tgtgcagcac ctgcggcgag cagggcagga attaaaagtg tcagtacttt gcttttcatt
     2221 gaataaatcc tttagttatt ttatttgcct tttatcccca tttcggggag tgaa
//
LOCUS       DAZFAH010000360         2217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFAH010000360 DAZFAH010000000
VERSION     DAZFAH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.363"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..83
                     /gene="ydfD"
                     /locus_tag="ACHJWB_004892"
     CDS             <1..83
                     /gene="ydfD"
                     /locus_tag="ACHJWB_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO4316346.1"
                     /translation="EQKIPGNCYPVDKVIHQDNNEIPAGL"
     gene            176..>2217
                     /locus_tag="ACHJWB_004893"
     CDS             176..>2217
                     /locus_tag="ACHJWB_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4316347.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDECRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPSIPRPALDSWDAEYMQENRWDEESASFVPVETESYPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NTMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT"
BASE COUNT          687 a          523 c          545 g          462 t
ORIGIN      
        1 ccgaacagaa aattcccggt aactgttacc cggtcgataa agttattcac caggataata
       61 acgaaatccc ggcaggtctt taaaacagtt ccgtaataaa catccgattt cattcttata
      121 tgccagcaat ggcagggatt tgttcaccct taaatctgta atgaggtaaa acaaaatgag
      181 taaagtcttt atttgcgccg ccattccgga cgaacaggca ataaaggaag aaggtgcagt
      241 cgctgtagcc actgccattg aagccggtga tgaatgtcgc gcccgcgcaa aatttcactg
      301 gcaattcctg gaacattatc cggctgctca ggactgcgct tataaattta ttgtctgcga
      361 ggataaacct agcatacccc gccctgccct cgattcatgg gatgctgaat atatgcagga
      421 aaaccgctgg gatgaggagt ctgcttcttt tgtcccggtt gagactgaat cctatccgat
      481 gaacgtcact tttgacaagc tggcccctga agtacagaac gctgtcatgg ttaagttcga
      541 cacatgtgaa aacatcaccg ttgatatggt tattagcgca caggaattgt tgcaggaaga
      601 catggcaaca ttcgacggac atatcgttga agcgttgatg aaaatgccag aagttaacac
      661 catgtatccg gagcttaagt tgcacgccat tgggtgggtt aagcataaat gtattcctgg
      721 tgctaaatgg cccgaaattc aggcagagat gcgcatctgg aaaaaacgtc gcgaaggtga
      781 acgcaaggaa accggaaaat acacgtctgt tgttgatctc gcccgcgcca gagccaatca
      841 acagtacact gaaaattcaa caggaaaaat cagcccggtc attgctgcca ttcatcgcga
      901 atacaagcag acatggaaaa cactggatga cgaactggcc tacgctctct ggcctggtga
      961 tgtggatgcc ggaaacattg acggcagcat ccatcgctgg gcaaaaaatg aagttatcga
     1021 caacggccgc gaagactgga agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct
     1081 tcgctacgac cgccaaacta tttttggcct tgtccgtgaa cgtccgatcg acattcacaa
     1141 agatcccgta gcactgaaca aatatatctg cgaatacctg acgacaaagg gcgtgtttga
     1201 gaatgaagaa acagacctgg gcactgttga tgttctccag tcatcagaaa cacaaactga
     1261 tgcagtggaa actgaggtat ctgatatccc aaaaaatgaa accgcgccgg aagctgaacc
     1321 atctgtagag cgtgaggggc cgttctactt cctcttcacc gacaaggatg gcgaaaaata
     1381 cggtcgcgca aacaaacttt ctggtctgga taaggcgctg tctgctgggg ctactgaaat
     1441 cacgaaagaa gaatatttcg cccgcaaaaa cggtacatac tcaggttcac aacaaaatac
     1501 tggtgcatct gacacgaccg cacaaccagg gtcagtaaaa gttaccgctg acgaagtaaa
     1561 caaaattatg caggcagcca atatcagcca gcctgacgcc gatgaactgc ttgcagtatc
     1621 acgtggtgaa tttgttgaag ggattagcga cccgaatgat ccgaaatggg ttaaggggat
     1681 ccagacccgc gattctgtga accagaacca gcaagaaacg gaacagaacg accagaaagc
     1741 ggaacaaaac agcccaaata cgcaacaaaa cgagccagaa acgaaacaac ctgaaccagt
     1801 agtgcaacag gaaccggaaa agatctgcac cgcctgcggt cagagcggtg gcggcaactg
     1861 ccctgattgt ggtgcggtga tgggtgacgc aacataccag gaaatattcg atggagagaa
     1921 tcagcctgaa gttcaggaaa atgatccgga ggaaatggaa ggcactgcgc atcagcacaa
     1981 ggagaacact ggcggcaatc agcatcatgc cagcgatagt gaaactggcg aggcgtcaga
     2041 tcccttaatt aaggcgaacg gtcatcataa tctcacatcc accagcagag cggggattca
     2101 tctgatgatc gaccttgaaa ccatgggaaa aaatcccgat gccccgatta tctcaatagg
     2161 tgcaatattt ttcgatccgc aaaccggaga tatgggaccg gaatttagta agactat
//
LOCUS       DAZFAH010000361         2214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFAH010000361 DAZFAH010000000
VERSION     DAZFAH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.364"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..334
                     /locus_tag="ACHJWB_004894"
     CDS             143..334
                     /locus_tag="ACHJWB_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4316348.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(387..620)
                     /locus_tag="ACHJWB_004895"
     CDS             complement(387..620)
                     /locus_tag="ACHJWB_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316349.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(716..1339)
                     /locus_tag="ACHJWB_004896"
     CDS             complement(716..1339)
                     /locus_tag="ACHJWB_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4316350.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1428..1937)
                     /locus_tag="ACHJWB_004897"
     CDS             complement(1428..1937)
                     /locus_tag="ACHJWB_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316351.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          637 a          587 c          470 g          520 t
ORIGIN      
        1 ggaggagcgc aataaaatac acaggaagaa ggggaaaatg tttcgtaaaa acagaaaatt
       61 aacagcgcac atcaggaaac gcagtctcct gattcagtgc taccacatga aaatttagtt
      121 aactgttacc gtactaaaat ttatgtcaga agatgttcca ttaccgaaag tcaatcagcg
      181 atacaaagac gatcatggtg cgcttgtcac cctgaccagt gttgaggaaa cccgggtggt
      241 gtttatgcag gacggctatc cgcatccctg catgcgaccg atgtacaact ttctgggaaa
      301 attcaaaccc gaaccccggg aagaaacaga ataaaacgaa atccctgccg gcaatcacac
      361 tacctgatag cagcataatc cacttattaa ttctcatccc cggagacaag cccagaaaac
      421 cactccgggt gttcactgta tgccgtgttc agcgcatccc tggaaacgtt tatataaaag
      481 ctgtcctgca atttgcgggt aacaacttct gccaggcgat tccggttatc caccagtccg
      541 gcataacctc tctctgccag ccactcattc agttcatgtt ccagagacag attgataata
      601 tcataatcgg accttgccat tcccgtttcc ttagcacatg ttttcaggca cggggataaa
      661 gtataaagaa tgtcatactg ctgctgccag agcaatattc cccctgatgg ttgtatcaga
      721 gatggtatgc cggtactttg cgggttgttc ccgtcaggtt atcgtgcacc tccacggtcg
      781 tggtcaccac cggcattccg gcctttctca gcctcagaac atcagccgca atacgctgac
      841 tgccaaacca gaacaggccg tccagtgcag tcaccagtaa acccgcctcc agtgcatact
      901 tcagccgttc acggggcgct ttcacttccc gggcaacctg ctgatatggc aatgatgtgt
      961 tttcattccc aaccacgcga cgaatacggt gagacagatg ataccggtat gtatccggca
     1021 caccagaaag gctggccttc aggctgtaca cgcagccaaa ccgtttatcg ttaaacacca
     1081 catttttctg gctgatgccc cattcttcac gcagcgcggc aatcaattgc ggtgtacggg
     1141 taagcagcaa gcgaaaaggc agttcaaaac tggtgacgta atccacattc agcagtgcaa
     1201 tacgcaaccg ttcttctggc ccggcctctg tctgccggca ctcctccaga acatcctgcc
     1261 actgccggcg aagacggggg gattcgttcc gttctgtaaa acagtattta tccgccagat
     1321 agtcaattcg tgtacgcata ctgaaaggaa ttccgtataa agattcagca gacaaaactt
     1381 tatcagccag taaaaattaa cggaagcatt gatattcctc ccgacaatca ccggatgatt
     1441 gttgcaataa cctgcgacat aaaggactga acagccgttt ttaacgccac gtattgccct
     1501 gatgaaccag gccgggccac ccgaaaacat ctttccaccc ggcattgtcc gccagcactt
     1561 atcaccgacc aggtctgttg cagtaaatcc accagttgcg cacattcttc atcagccatg
     1621 acgccgactc gcggatggca aatactcgtc tggctgagca gcaacagaca tcgtatctgt
     1681 gctccttcat gttgttcccg caaccagcgt aataccaccg gcgctgatgg gctgaccgtg
     1741 tgattatgct ccgtaatacg ttcgtgtacc cgtcggtgaa aggaactaca aaatgtctga
     1801 atctgttgac catagattcc ttctgccgaa tagagcgtga agtgaaatgc caacagattt
     1861 ggccagtggt ccaccgcctg ctgaacaaaa cgccggattt cccccggctc tgaaattaag
     1921 gcttcggtta tttgcacgaa atgttccttt tcaacccctg ttgaattttg tcaagccggt
     1981 gcagacacag taaagcgcgc atcaacacct gaaaggtacg atacaaacac aagttacaaa
     2041 caataagaac ggaaggacaa ttaatcagtg aggaataata actaaattaa ccaaaagaat
     2101 gttactgaca atactaccaa tacttaaata gcaatatatt aattaatgta agaacagatg
     2161 cccggtagga catatacaca atacatccac aaacacactc ttttcattaa atca
//
LOCUS       DAZFAH010000362         2188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFAH010000362 DAZFAH010000000
VERSION     DAZFAH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.365"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(181..720)
                     /locus_tag="ACHJWB_004898"
     CDS             complement(181..720)
                     /locus_tag="ACHJWB_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4316352.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(822..2033)
                     /gene="mhpT"
                     /locus_tag="ACHJWB_004899"
     CDS             complement(822..2033)
                     /gene="mhpT"
                     /locus_tag="ACHJWB_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4316353.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYRS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
BASE COUNT          555 a          667 c          510 g          456 t
ORIGIN      
        1 tctgcactgg agggataata caaggcctga taagacgcgc aaagcgtcgc atcaggcatg
       61 gcgcacgact gccggatgcg gcgtgaacgc cttatccggt ctacacttcg cccataaacc
      121 gtaggcctga taagacgcgc atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt
      181 ttaccacatc aaatcatcag gcactttgaa gtcggcatac ggatcgtctt catcctgctc
      241 ttcggcactg agcgcgctgt gcaacacaat actgctggca tcacgctgag caattttatc
      301 ggcaacactg gcgggaataa tggtgtattc accttcgcta ttgttatcga ccaacaagcg
      361 ggcaatcgcc agacgaccat taatcaactg cgcctgtgtg agcttatcga caaaaatctt
      421 cttaatcaga ttgccgtcag tgaagttaaa accaatatcg ccattggcaa tggtgattcg
      481 gttcatttca atgagctgct tcacctgggc tttatattct ttcgccaacg ccgcttgttt
      541 ttgctgttcg ctaagctgtt tatcacgctc aagctgtgcc tttttatttt cttctaccgc
      601 agcccgagct tcacgcgcct gaacgcgcga ttttttcgcc gttctctcga ccttcgccgc
      661 ttttttgctg gtgactaatc ctgctttgag caactgctct tgtaaggtaa gttttgccat
      721 ctttgtttcc aaacccgctg aataattttt agcgagtata cctgtaaacg ccgtgggtgt
      781 attgggggca agtccctttc gcccctgctt aataaggcgc gtcaggcatc ggcacacggc
      841 tgcattcttg atctccgact catcaaaata aacaccgcca acccagcaac aagaataccc
      901 ggtgcagaag cggccattac gccgaccgtg ccagtgccta atgccagcat tttcccggcc
      961 agtaacggac cgctcatagc ccccagacgc cctaccgcca cggctgttcc cacacctgtt
     1021 gcgcggatct gcgacctgta aaacaacggt gccagggcat acaaaacgct ttgcccacct
     1081 gtcgcaaaca accccgcgac aaatcccgcc agcaacatac cgttaaatga cgacaccgtt
     1141 ccaagcgcca gcagcgaagc taacatgccg ctataaatca gtagcgacat ggttactgga
     1201 cgcagcttat ccatcaatgc gcccaacatt aacgtcccgc ttgccgcccc catttgcagg
     1261 gcaaacatca cccctgccgc ctgcgatggc tggaatcctt gctccaccaa aagtagcggt
     1321 agccagttga tcaacatata aaccaccagc agagtgaaga aataacacaa ccacagcagc
     1381 agcgtcgcgg ttgccgtttc tggcgcaaat aaggcacgta gtggtggcgc agcctgtttt
     1441 tcgccagcga aaaccgctga ctccggcagc cagcgcatta atagcggcac cagaatcaac
     1501 ggcaccacac cacctaccca aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc
     1561 agtgtcgccg ccagcgccgc gccaatggga acaccgcaat acatcaggct cactgccgtc
     1621 ccacgaaaac gtggacccgc ggcttcagac gtcagggcga taagattcgg caacgccgcc
     1681 cccagcccga caccggtcat cagccgcgca aagaccagtg aggggaaatc ccaggcaatc
     1741 gccgttgcca gtgagaacaa accaaacagc gcaactgagc caatcaaaat acgcttacga
     1801 ccataacgat ctgccagcat cccgccaacc aacgcgccgg gtagcaaacc gagtattccg
     1861 gcgctaaata tccagcccat ttgcatttta tcgagtgcga aagcctgggc gatgccaccc
     1921 gccgcaatgc cagccgcctg aagatccagc ccttccatca gagcgaccaa aaaacaaagc
     1981 ccgatagtca gcatcaggcg ggatgaagat gatgaagggg tacgagtcga cataagatga
     2041 acctcgttgc gagtgcaggt gaatcgcgac tgatacattg ccagccgcgt aatcagaact
     2101 atttaatgac tgcctacggg cgaaatgtag gccggacaag acgtttaccg catccggcag
     2161 tcgtgtgcag atgcctgatg cgacgcta
//
LOCUS       DAZFAH010000363         2167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFAH010000363 DAZFAH010000000
VERSION     DAZFAH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.366"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..293)
                     /locus_tag="ACHJWB_004900"
     CDS             complement(<1..293)
                     /locus_tag="ACHJWB_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316354.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVG"
     gene            complement(445..816)
                     /locus_tag="ACHJWB_004901"
     CDS             complement(445..816)
                     /locus_tag="ACHJWB_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4316355.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(806..1177)
                     /locus_tag="ACHJWB_004902"
     CDS             complement(806..1177)
                     /locus_tag="ACHJWB_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4316356.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(1190..2167)
                     /locus_tag="ACHJWB_004903"
     CDS             complement(1190..2167)
                     /locus_tag="ACHJWB_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4316357.1"
                     /translation="MRVFHGGRVLVESEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     NERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVS
                     RRSLGLPAEKIRSVYRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVS
                     IAVDPESPESFMKRPKRRRWVNEKYTCWVKTQPCACCGKPADDPHHLIGHGQGGMGTK
                     SHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          606 a          576 c          443 g          542 t
ORIGIN      
        1 ccaacagtaa cagaatttag cactacagga ttgtgaaacc ctggccatag atggaaagat
       61 aatgctatgg ctgataaaac tatgccaact tcatatatag atttagccca ggcgttatat
      121 ttccagttga acttcaaaat aataaaaaaa acgattgtag caacaaaaaa taacaccgac
      181 caatcaataa tatcattaag cacagccagg acaacagaca ccgtcaacaa tgaaaaagcc
      241 actaccttat gccaactgag agttgacagt gacagtacta acacaatcca cataagcact
      301 ccttttattt aatgatgaag attgattatc aatattttca attcaccagg caacatttta
      361 tctaccttcc acaatacatg accatcaaaa aataaacatg ttaattataa acacagaaaa
      421 cataaccctc atcactatat atccctaccg catatccata tctaaccgga cattcagagc
      481 cataagcatt ccttcaataa taccttccgc tttataaagc cttttaccaa taagcccatc
      541 agaacatcta tgcttacgtg caagagccat aaatgtcatt ccacctacgt aataatccac
      601 caacaaatcg tgcaaatact gattattctt gtttaatcgt gccatacaac cacaaattat
      661 catggcgtca tcatcagaac actgaggacg tgatttcact ttagtcggga ttaatccttt
      721 aaaaccagca gctatcgagg gccatgttac atcttcatga ttatttgctg cccatgcccc
      781 ccatcgctca agaaccatct ggatatcacg cgccatcgtt accacctctg atttcgtaaa
      841 tcttcacgcc cagccgtcca cctggcacag gctgaccgcg cacaatattg atttcatcaa
      901 actgctcgtc atcaatgagc acttccgcat gcgtcagcgc atccagcggt gctttcagaa
      961 tgttgtccag gtcacggcgg cgcttatccg gtggttctgc aataattttt attgccagcc
     1021 gtccggacag gcttaatttc agccgctgct ggcgaacaat aagcgccaca gcccggcgat
     1081 aacgctcacc ggcttttgat acaaaatatg tgctgccacg acgtcgccag taggtgttca
     1141 ccgtcggcgg gtaaggcaaa acaaactcta tacgcatcag taacctcttt tacccgagca
     1201 cgccggttgc aaaagcgtga tcaagaaaac gaaaaattaa atcaacctgg gaaccatgct
     1261 tttcttcgaa cgccagcgga tccgcatgaa gctcgttgtg atgctcccga cacagcggta
     1321 gcgtgaaaat atcgtgggat tttgttccca ttccaccctg accatgacca atcagatgat
     1381 gaggatcgtc cgctggctta ccacaacacg cacacggctg tgtctttacc cagcatgtgt
     1441 atttctcatt tacccagcgg cgacgtttag gtcgtttcat gaaggattcc ggagactccg
     1501 gatcaacggc aatgctgacc accgtctttt cctgtggtgg gttctgttgc tggtgggtgt
     1561 gaggcggtag cgcaatattt tttgtgcgct gcttcagtat gctggtggcg gtctgttctc
     1621 ccggtatgat gtcgctttca cggtatacgg agcggatttt ttccgccggt aatcccagag
     1681 aacgacgcga tactgcctca ggtaatgcgt ccaccacctg attgcagact gcccaccagg
     1741 ataattcagc cagcgataat tcccgttcct gtatgccatt cattgcgtgg cggatgacgt
     1801 caatcatcca tgctgacagg ttttgttgag caagttgctc aagtgattcg gatgtctggt
     1861 cgcgcagctg gttgtcgcag tgccagcaca acaccatcgc gccggtaccg taacgatgta
     1921 tgacagtttc gctgtgatga taatcaccat gaggccactg gcaggattta acgtgacgta
     1981 ataaccagtc agacagtgca ccagcgccac cagcagcacg aatcacacgc tcattgctga
     2041 aaaatggcag taatgattta tcttccgcca gcggctggcg aacggcagga acgaccccgg
     2101 acggcagatt acgcatgctt ttcggttcag actccaccag caccctgccg ccatgaaata
     2161 cccgcat
//
LOCUS       DAZFAH010000364         2167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFAH010000364 DAZFAH010000000
VERSION     DAZFAH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.367"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..2151
                     /gene="yjcS"
                     /locus_tag="ACHJWB_004904"
     CDS             166..2151
                     /gene="yjcS"
                     /locus_tag="ACHJWB_004904"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4316358.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKQEEIIACLDNFDLWVNIVTPN"
BASE COUNT          575 a          576 c          528 g          488 t
ORIGIN      
        1 cgcgtaattt tcccccttca gcacaaatgc ctaataaaat ggatcgttcc gctcacactt
       61 ccacttatta aagtcgcaaa ttcccaatta tgataacccc agctttaaat gacgtatttt
      121 gcatggatta catatttacg acataaatga taaatggaga tttatatgaa taactctcgg
      181 ttattccgtt tgagcagaat tgttattgcg ttaactgccg ccagcggcat gatggtaaat
      241 accgcttacg caacagatga agcgaaagcc gccactcaat atacccaaca ggttaatcag
      301 aattacgcca aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga
      361 tttatcgctc cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat
      421 cgcggggaag attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc
      481 ctgtggcgtc agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg
      541 taccaggttc gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt
      601 attgtcatcg acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc
      661 caaaatcgcc cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac
      721 tatggcggcg tgaaaggcat tatctctgaa gccgatgtga aatccggtaa agtgcaggtt
      781 atcgcccctg ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt
      841 atgagccgcc gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac
      901 atcggcaatg gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac
      961 aaaaccatca ccaaaaccgg tgagaagatg attatcgacg gcctggagtt tgacttcctg
     1021 atgaccccag gtagcgaagc accagccgaa atgcacttct atattccggc cctgaaagcg
     1081 ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg
     1141 aaaacccgcg acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt
     1201 aacgacgcgg aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc
     1261 aatgattata ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac
     1321 ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca
     1381 cttgccaata actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg
     1441 gtgtataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt
     1501 caggtggaga tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac
     1561 ctggcgcaag aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag
     1621 gtgattgccg ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa
     1681 cagctgggct atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa
     1741 gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt
     1801 cgcgggatgt cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa
     1861 gccgcgggta aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg
     1921 acgctgaacg atagcgtgct taactaccgt aaaacgctgc aaccgcaagc tgacgcctct
     1981 ttctacatca gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat
     2041 ctggtaaaag cgaagaaagc caaaattatt ggcaatggcg cgaaacagga agaaattatc
     2101 gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg
     2161 cttttgg
//
LOCUS       DAZFAH010000365         2160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFAH010000365 DAZFAH010000000
VERSION     DAZFAH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.368"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..1662
                     /gene="tir"
                     /locus_tag="ACHJWB_004905"
     CDS             13..1662
                     /gene="tir"
                     /locus_tag="ACHJWB_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO4316359.1"
                     /translation="MPIGNLGNNVNSNHLIPPAPPLPSQTDGAARGGTGHLISSTGAL
                     GSRSLFSPLRNSVADSVESRDIPGLPTNPSRLAAATHETCVLGGFEVLHDKGPLDILN
                     MQIGPSAFRVEAQADGTYAAIGEKNDLEVSVTLSPQELSSLQAIDTEGKNRFVFTGGR
                     GGSGHPMVTVASDIAEARTKILAKLDPGNQGGRQAKDVDTRSVGVGSASGMDDGVVSE
                     THTSTTTSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAAN
                     AAESATKDLLTQEAFKNPENQKVDIDENGNAFPSGVLKDDIVAQIAEQAKEAGEVARQ
                     QAVESNAQAQQRYDDQHARRQEELQLSSGIGYGLSSALIAAGGIGAGVTIALHRRNQP
                     TEQTTTTTHTVVLQQTGGNTPAQGGTDATRAEDASLNRRDSQGSVASTHRSDSSSEVV
                     NPYAEVGGARNSLSAHQPEEHIYDEVAADPSYSVIQHFSGSGPVTGRLIGTPGQGIES
                     TYALLANSGGLRLGTGGLTSGGESAVSSITAAPTPGPARFV"
     gene            1794..>2160
                     /locus_tag="ACHJWB_004906"
     CDS             1794..>2160
                     /locus_tag="ACHJWB_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CesT family type III secretion system chaperone"
                     /protein_id="HFO4316360.1"
                     /translation="MSSRSELLLDRFAEKIGVGSISFNENRLCSFAIDEIYYISLSDA
                     NDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLD
                     DATPEKLENEIEVVVKSMEN"
BASE COUNT          595 a          433 c          567 g          565 t
ORIGIN      
        1 aaggatatat gtatgcctat tggtaacctt ggtaataatg taaatagcaa tcatttaatt
       61 ccccctgcgc cgccactacc ttcacaaaca gacggcgcgg cacggggagg aactggtcat
      121 ctaattagct ctacaggagc attaggatct cgttcattgt tttctcccct gagaaattct
      181 gtggctgatt ctgtcgagtc cagagatatt ccaggacttc ctacaaaccc atcgaggctt
      241 gctgcagcta cacatgagac atgcgtgctt ggaggatttg aagttctcca tgataagggg
      301 ccacttgata ttctcaatat gcaaattgga ccctctgcat ttcgtgttga ggcgcaggca
      361 gatggtactt atgccgctat tggagaaaaa aatgatttgg aggttagcgt tacattaagt
      421 cctcaagaat tgagcagctt gcaagctatt gatactgagg gtaaaaacag atttgttttt
      481 accgggggac gtggcggtag tgggcatccg atggtcactg tcgcatcaga tatcgcggaa
      541 gctcgtacga aaatactggc caaattagac ccaggcaatc agggagggcg tcaagccaag
      601 gacgttgata cgcgttctgt tggtgttgga agcgcttcgg gaatggatga tggcgttgtt
      661 agcgaaaccc atacttcaac aacaacttcc agcgttcgtt cagatcctaa attctgggtt
      721 tctgtcggcg caattgctgc tggtttagcg ggactggcgg caactggtat tgcacaggcg
      781 ttggctttga caccggaacc ggatgatcct acaaccaccg atcctgatca ggccgcaaat
      841 gctgcagaaa gtgcaacaaa agatctgtta acgcaagaag cattcaagaa ccctgagaac
      901 cagaaagttg acatcgatga gaacggaaat gcctttccgt ctggggtatt aaaagatgat
      961 attgttgcgc aaatagcaga acaagctaaa gaggctggtg aggtggccag acagcaggct
     1021 gttgaaagca atgcacaggc gcagcagcga tatgatgatc agcatgccag acgtcaggag
     1081 gaattacagc tttcgtcggg tattggttac ggcctcagca gtgcattgat tgctgctggg
     1141 ggaattggtg ctggtgtaac gattgcgctc catagacgaa atcagccaac agaacagacg
     1201 actacaacaa cacatacggt tgtgctgcag cagaccggag ggaatacccc agcacaaggt
     1261 ggcactgatg ccacaagagc agaagacgct tctctgaata gacgtgattc gcaggggagt
     1321 gttgcatcga cacacaggtc agattcctct agcgaagtgg ttaatccata tgctgaagtt
     1381 gggggggctc ggaatagtct atcggctcat cagccagaag agcatattta tgatgaggtc
     1441 gctgcagatc ctagttatag cgttattcaa catttttcag ggagcggccc agttaccgga
     1501 aggttaatag gaactccagg gcaaggtatc gaaagtactt atgcgcttct ggcaaacagc
     1561 ggcggattgc gtttaggtac gggaggatta acgagtggtg gcgagagcgc agtaagttct
     1621 ataactgccg caccaacgcc gggacccgca cgtttcgttt aaatatatct gtgagtattt
     1681 agttgaggtt ggggtggggg cgttttacta gcgttaatgt ttcagagaac aacgttgcag
     1741 catgggtaac tcttgaaatt ctgttattat aaatccaatt aagagaaatt ataatgtcat
     1801 caagatctga acttttatta gataggtttg cagaaaaaat tggtgttgga tctatttcct
     1861 ttaatgaaaa tagattgtgt tcttttgcta ttgatgaaat ttattatatt tcgttatctg
     1921 atgccaatga cgaatatatg atgatttatg gcgtctgtgg aaaattcccg acagataacc
     1981 ctaacttcgc tcttgagatt ttgaatgcaa acttatggtt tgcagagaat ggtgggccat
     2041 acctgtgtta tgagtcagga gcacaatcgc tgttgttagc gttacgtttc ccgctcgatg
     2101 atgctacccc tgaaaaactt gagaatgaaa tagaagtcgt cgttaagtcg atggaaaacc
//
LOCUS       DAZFAH010000366         2127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFAH010000366 DAZFAH010000000
VERSION     DAZFAH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.369"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..236
                     /gene="ymgC"
                     /locus_tag="ACHJWB_004907"
     CDS             <1..236
                     /gene="ymgC"
                     /locus_tag="ACHJWB_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO4316361.1"
                     /translation="PERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSV
                     ILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            568..2091
                     /locus_tag="ACHJWB_004908"
     CDS             568..2091
                     /locus_tag="ACHJWB_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4316362.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLKLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          578 a          449 c          468 g          632 t
ORIGIN      
        1 tcccagagag atttattttt caatgtgctt tgtttaaaaa tctcgagagg gaggtgttca
       61 tgacgcatgg gtatgttgat agtcatatta ttgatcaggc tttacgcttg cgtttaaaag
      121 atgaaaccag tgtgattctt tctgatctct atctgcaaat cttgcagtat attgaaatgc
      181 ataagactac actaacggat atcattatta atgacaggga atccgtgctc tcttagaact
      241 tgagtacggt tatagaataa acatcagctg tatcatcatg ctggtgcaaa gtgagcattt
      301 caggcgttat gctttcttaa tatatccgca atatcagttg tcaagaatgg agagttctcg
      361 ttctccattc ttgatgcctg atatcccacc taatttattt aatcctgcct acattatgct
      421 caataattaa tgggttagat caagatggag aaaattttat cttcggcgtg gatatttcct
      481 atgctctcaa ccatgctctc tgtagatgcc agcgcgagcg aatgaatggc attttcatgt
      541 ataaaaccct ttggccggga caagcaaatg cgcaatacac tcatacccat cctcgttgcg
      601 atatgcttgt ttataaccgg tgtcgctatt ttaaacatcc agctctggta ttccgcaaaa
      661 gcagagtacc tggcgggagc gagatatgcc gccaacaata tcaatcatat acttgaagaa
      721 gcgtcacagg cgactcaaac agcggttaac attgccggga aggaatgcga cctcgaggag
      781 caatatcagc ttggcactga agcagctctg aaacctcacc tgcgcacaat catcattctc
      841 aaacagggaa tagtctggtg tacatccctg cctgggaatc gggtcctgct gtctcgtatt
      901 cctgttgtcc cggacagtaa tttactgttg gctccggcaa tcgacaccgt taatagatta
      961 cctatcctgc tctatcagag ccaatttgca gatacgcgca ttttggttac gataagtgat
     1021 caacatattc gcggggcgct taatgtaccc ttgaaagggg taaggtatgt attacgcgtg
     1081 gcggatgaca ttattggacc aacgggtgat gtgatgacgc ttaatggaca ttatccctat
     1141 accgagaagg ttcactccac aaaatatcat ttcactatta tctttaaccc gccgccactc
     1201 tttagcttct acagacttat cgataaaggc tttggaattt taatatttat tctgttaatc
     1261 gcctgcgccg ctgccttcct gcttgataga tatttcaata aaagcgcaac gcctgaagag
     1321 atcctgcgac gggcaataaa taatggggag atcgtccctt tttaccaacc tgtggtaaat
     1381 ggtcgggaag ggacattgcg gggagttgag gtgttagccc gctggaaaca acctcacggt
     1441 ggatatatat cacccgcggc atttattcca cttgctgaaa aatccggatt aatcgttccg
     1501 cttacgcaaa gcctgatgaa tcaggttgcc agacagatga acgctatcgc gagtaaattg
     1561 ccggaaggtt ttcatattgg aattaatttt agcgcttcgc atattatttc gccgacgttt
     1621 gtcgacgagt gtttaaattt ccgtgacagt tttacccgcc gcgatttaaa gcttgttctg
     1681 gaagtcaccg agcgtgagcc attgaatgtt gatgaaagtc tggttcagcg gttgaacatt
     1741 ctgcatgaaa atggttttgt catcgcgctg gatgatttcg gtactggcta ctcagggctt
     1801 tcttatcttc atgacctgca tattgattat atcaaaattg atcacagttt cgttggccgc
     1861 gtaaacgcag acccagaatc aacccgaatt ctggattgtg tattggatct ggcgcgtaaa
     1921 ctttcgatca gtatcgtcgc tgaaggtgtc gaaacgaaag aacaacttga ctatctgaac
     1981 caaaataata tcacatttca gcagggttat tatttctata aacctgttac atacatcgac
     2041 ctggtcaaaa ttatcctttc taaaccgaag gtgaaggttg tggttgagtg aaaattgatc
     2101 agtaaggcca tagtgcggtg taattat
//
LOCUS       DAZFAH010000367         2124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFAH010000367 DAZFAH010000000
VERSION     DAZFAH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.370"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2124
                     /locus_tag="ACHJWB_004909"
     CDS             <1..>2124
                     /locus_tag="ACHJWB_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4316363.1"
                     /translation="LASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIID
                     VKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLK
                     PAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGFGGTEPRM
                     TFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVADNE
                     GVGFRYSFSALKDRHTAVEVSYVDPHNGWQTSTELVEDPEAILRYGRNLLKMDAFGCT
                     SRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLS
                     IDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVET
                     YGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLN
                     SVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGQDSRLVTTAIT
                     ADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITA
                     VPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYV
                     RSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEM
                     KTTITETRNEITQTVSKTLEDQSAT"
BASE COUNT          469 a          593 c          672 g          390 t
ORIGIN      
        1 cctcgcttcg gtgattctgg ataatctgcc tccccgcccc tttaacatcc ggatggtcag
       61 ggagacggcg gacagcacca cggaccagct gcagaataag acgctctggt cgtcatacac
      121 cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg attgtggggc tgcaggtgga
      181 tgcggagcag tttggcggtc agcagatgac ggtgaactac catatccgcg gtcgcatcat
      241 tcaggtaccg tcaaactatg acccggaaaa acgcacgtac agcggcatct gggacggcag
      301 cctgaaaccg gcatacagca acaacccggc ctggtgcctg tgggacatgc tgactcaccc
      361 gcgctacggc atgggaaaac gcctgggggc cgcggatgtg gacaagtggg cgctgtatgc
      421 catcgggcag tactgtgacc agcgtgtccc ggatggtttc gggggcacag agccgcggat
      481 gacctttaat gcgtacctgt cacaacagcg taaggcgtgg gacgttctca gtgatttctg
      541 ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgtttg tgcaggaccg
      601 tccgtcagat gtggtgtggc cctacacaaa cagtgatgtg gtggctgaca atgaaggtgt
      661 gggattccgc tacagtttca gcgccctgaa ggaccgtcat actgcggtgg aggtcagtta
      721 cgtcgacccg cataacggct ggcagacctc cacggaactg gtggaagacc cggaagccat
      781 actgcgctac gggcgcaacc tgctgaagat ggatgcgttc ggttgcacca gtcgcggtca
      841 ggcccaccgt gccgggctgt gggtgataaa gaccggactg ctggaaacgc agacggtgga
      901 tttcacgctc gggtcacagg ggctgcgtca cacacccggt gacattattg aaatctgtga
      961 taacgactat gccgggacca tgaccggcgg acgtgtcctg tccatcgatg ccgccagccg
     1021 caccctgacg ctggaccgtg aggtgacact gccggagacc ggtgccgcca cggtgaacct
     1081 gattaacggc agcggtaagc cggtgagtgt ggacatcacc gcacaccccg cgccggaccg
     1141 gatacaggtc agcaccctgc ctgatggtgt ggagacatac ggtgtatggg gactatccct
     1201 gccgtcactg cgccgtcgcc tgttccgctg tgtctccgtc cgggaaaaca cggacggcac
     1261 ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa gccatcgtgg ataacggtgc
     1321 ccgctttgag ccgcagtcag gcaccctgaa cagcgtcatc cctccggcag tgcagcacct
     1381 gacggtggag gtgagcgcag ctgacggcca gtatctggcg caggtgaaat gggacacgcc
     1441 gcgggtggtg aagggcgtgc gcttcagtct gcgcctgacc agtggaagcg gtcaggacag
     1501 ccgtctggtg accaccgcca tcaccgcgga tacagagcac cgtttcagcg gtctgccgcc
     1561 gggggaatac accctgacgg tcagggcgat taacagttat ggccagcagg gcgaaccggc
     1621 caccaccacc ttccggatta acgcgccagc aaaacccgcc accattgaac tgacgccggg
     1681 gtattttcag ataacggcgg tcccgcgtct tgcggtgtat gacccgacgg tacagtttga
     1741 gttctggttt tcggagacaa aaatcgcaga tacatctcag gtggaaacct ctgcccgtta
     1801 tctggggacc ggcagtcagt ggagtgtatc cggcccgcac attaagcccg ggaaggattt
     1861 ctggttttac gtgcgcagcg tcaacctggt ggggaaatct gcttttgtgg aagccagtgg
     1921 ccgggccagc aatgatgcag aagggtatct ggggctgttt cgggaaaaaa taggaaaact
     1981 gcatctggct caggggctgt gggagctgat agacaacagc cagcttgcgg atgagatggc
     2041 ggagatgaag accaccatca ccgaaacccg caatgaaatc acacagacgg tcagtaaaac
     2101 gctggaagac cagagcgcca ccat
//
LOCUS       DAZFAH010000368         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFAH010000368 DAZFAH010000000
VERSION     DAZFAH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.371"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..516
                     /locus_tag="ACHJWB_004910"
     CDS             <1..516
                     /locus_tag="ACHJWB_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316364.1"
                     /translation="LRRDRLINRTFPDNTGRSARRARASGDVALWLVQTDAFKDRVNN
                     ALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGANEAFDLLVYADALA
                     VLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEKRRRRKKKLWTERAE
                     DNPWITSGGWL"
     gene            513..725
                     /locus_tag="ACHJWB_004911"
     CDS             513..725
                     /locus_tag="ACHJWB_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316365.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            725..>2058
                     /locus_tag="ACHJWB_004912"
     CDS             725..>2058
                     /locus_tag="ACHJWB_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316366.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVRGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRA"
BASE COUNT          474 a          475 c          677 g          432 t
ORIGIN      
        1 cttcgccgtg acaggctgat taaccgaacc ttcccggata ataccggcag aagtgcccgc
       61 cgtgccagag ccagtggcga tgtcgcgctg tggctggttc agacggatgc gtttaaggat
      121 cgtgtaaata atgccctgtg gcgtgacaca ccagggccga actatatcca ctttcccgac
      181 tggctggggc ggtggtttta cgatgagctg acctatgaag agcgcggcag tgacggaaaa
      241 tggcgaaaac cgggcagggg cgctaacgaa gcgtttgacc tgctggttta tgcggatgcg
      301 cttgccgttc tgcatggtta cgaaaagatc cgctggccct ccgcaccgga ctgggcacag
      361 cgggaaacgt ggctcgtctt cccgcaggag cgttctggtg aaacggtatc cccggaactg
      421 acggccgggg cagaaaaacg ccgtcgccgg aagaaaaaac tgtggacgga gcgtgcggaa
      481 gataatccat ggataacatc aggaggctgg ttgtgagcac agaagaagcc agagaaatga
      541 tacagcggta ccgtgaagcg gaaatggccg tactggaggg aaagtctgtc accttcaacg
      601 ggcagcaact gacgctggaa agcctttctc agatccgcgc cggacgtcag gagtgggaac
      661 gcaggcttgc cgcgatggtg agccgcaggc ggggaaaacc gggatttaaa ctggcgaggt
      721 tttaatggca attattgatg atgtgatcgg cgtgttttcc cccgggtgga aagcagccag
      781 actgcgttca agggcgttaa tcatggccta tgaggcggtg aaaccgaccc ggacacataa
      841 agcccggcgg gaaaatcgct ctgctgatca gctcagtaaa tacggtgcgg tttccctgcg
      901 ggagcaggcc cgttttctgg atatcaatca tgacctggtg attggtgtgt ttgacaagct
      961 ggaagagcgg gtgattggtg ccaggggaat tattgtggag cctcagccat tacgaaaaaa
     1021 cggggaaatg gcggctgagc tggctgcgga tatccgccgt ttgtgggctg aatggtccgt
     1081 gagtccggat gtgacagggc agtatacccg tcctgtgctt gaacgtttac tgctgcggac
     1141 ctggctgcgg gatggtgaag tgtttgcgca gatggtcagt ggtgcgggaa acggtctgga
     1201 acggacggcg ggagtgccat tctggcttga ggcgatggag ccggattttg ttcccatgcg
     1261 cactgatgaa tccgccggac tgaatcaggg ggtttttctt gatgagtggg gaagaccgaa
     1321 aaaatatctg gtttataaaa attatccggt cagaggccgg cagagtgata cgaaagaaat
     1381 cgctgccgga aaaatgatcc acctgaagtt cactcgtcgt ctgcatcaga cgcgaggctc
     1441 atccatgtta tcgggggtgc tgatgcggat cagtgccctt aaggagtatg aggatgcgga
     1501 actgacagcg gcgcgtattg ctgcggcgct gggactgtat atccgtaaag gtgacggaca
     1561 ggactatgaa gatccgggga gcaaagagac cgagcgggaa gtccatatca ccccgggtat
     1621 tatttatgac gatttgcgca agggcgagga tatcggcatg gtcaaatctg accgtcccaa
     1681 tcccaacctt gaaactttcc gcaacggcca gttgcgtgca gtggcagcag gcagtcgtct
     1741 gagtttttcc agtgcggcgc gtaactataa cggcacctac agcgcccagc ggcaggagtt
     1801 ggtcgagtcc acggatggtt acctgatcct gcaggactgt tttattggcg cggtaacccg
     1861 cccggtgtac cggacatggc tgaatatggt ggttgcggca ggtctgctga aaattccggc
     1921 ggatgtggag atgaaaacgc tatataacgc gacgtattcc ggtccggtga tgccgtggat
     1981 cgacccggtt aaggaagctg aagcctggag aattcagatc cggggtggtg cagcgacaga
     2041 atctgactgg gtgcgtgc
//
LOCUS       DAZFAH010000369         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFAH010000369 DAZFAH010000000
VERSION     DAZFAH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.372"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..516
                     /locus_tag="ACHJWB_004913"
     CDS             <1..516
                     /locus_tag="ACHJWB_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316367.1"
                     /translation="LRRDRLINRTFPDNTGRSARRARASGDVALWLVQTDAFKDRVNN
                     ALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGANEAFDLLVYADALA
                     VLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEKRRRRKKKLRTERAE
                     DNPWITSGGWL"
     gene            513..725
                     /locus_tag="ACHJWB_004914"
     CDS             513..725
                     /locus_tag="ACHJWB_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316368.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            725..>2058
                     /locus_tag="ACHJWB_004915"
     CDS             725..>2058
                     /locus_tag="ACHJWB_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316369.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNREM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRA"
BASE COUNT          475 a          479 c          676 g          428 t
ORIGIN      
        1 cttcgccgtg acaggctgat taaccgtacc ttcccggata ataccggcag aagtgcccgc
       61 cgtgccagag ccagtggcga tgtcgcgctg tggctggttc agacggatgc gtttaaggac
      121 cgtgtaaata atgccctgtg gcgtgacaca ccagggccga actatatcca ctttcccgac
      181 tggctggggc ggtggtttta cgatgagctg acctatgaag agcgcggcag tgacggaaaa
      241 tggcgaaaac cgggcagggg cgctaacgag gcgtttgacc tgctggttta tgcggatgcg
      301 cttgccgttc tgcatggtta cgaaaagatc cgctggccct ccgcaccgga ctgggcacag
      361 cgggaaacgt ggctcgtctt cccgcaggag cgttctggtg aaacggtatc cccggaactg
      421 acggccgggg cagaaaaacg ccgtcgccgg aagaaaaaac tgcggacgga gcgtgcggaa
      481 gataatccat ggataacatc aggaggctgg ttgtgagcac agaagaagcc agagaaatga
      541 tacagcggta ccgtgaagcg gaaatggccg tactggaggg gaagtctgtc accttcaacg
      601 ggcagcaact gacgctggaa agcctttctc agatccgcgc cggacgtcag gagtgggaac
      661 gcaggcttgc cgcgatggtg agccgcaggc ggggaaaacc aggatttaaa ctggcgaggt
      721 tttaatggca attattgatg atgtgatagg cgtgttttcc cccgggtgga aagcagccag
      781 actgcgttca agggcgttaa tcatggccta tgaggcggtg aaaccgaccc ggacacataa
      841 agcccggcgg gaaaatcgct ctgccgatca gctcagtaaa tacggtgcgg tttccctgcg
      901 ggagcaggcc cgttttctgg atatcaatca tgacctggtg attggtgtgt ttgacaagct
      961 ggaagagcgg gtgattggtg ccaggggaat tattgtggag cctcagccat tacgaaaaaa
     1021 cagggaaatg gcggctgagc tggctgcgga tatccgccgg ttgtgggctg aatggtccgt
     1081 gagtccggat gtgacagggc agtatacccg tcccgtgctt gaacgtttac tgctgcggac
     1141 ctggctgcgg gatggtgaag tgtttgcgca gatggtcagt ggtgcgggaa acggtctgga
     1201 acggacggcg ggagtgccat tctggcttga ggcgatggag ccggattttg ttcccatgcg
     1261 cacagatgaa tccgccggac tgaatcaggg ggtttttctt gatgagtggg gaagaccgaa
     1321 aaaatatctg gtttataaaa attatccggt cagcggccgg cagagtgata cgaaagaaat
     1381 cgctgccgga aaaatgatcc acctgaagtt cactcgtcgt ctgcatcaga cgcgaggctc
     1441 atccatgtta tcgggggtgc tgatgcggat cagtgccctt aaggagtatg aggatgcgga
     1501 actgacagcg gcgcgtattg ctgcggcgct gggactgtat atccgtaaag gtgacggaca
     1561 ggactatgaa gatccgggga tcaaagaaac cgagcgggaa gtccatatca ccccgggtat
     1621 tatttatgac gatttgcgca agggcgagga tatcggcatg gtcaaatctg accgtcccaa
     1681 tcccaatctt gaaactttcc gcaacggcca gttgcgtgca gtggcagcag gcagtcgtct
     1741 gagtttttcc agtgcggcgc gtaactataa cggcacctac agcgcccagc ggcaggagct
     1801 ggtcgagtcc acggatggtt acctgatcct gcaggactgt tttattggcg cggtaacccg
     1861 cccggtgtac cggacatggc tgaatatggt ggttgcggca ggtctgctga aaattccggc
     1921 ggatgtggag atgaaaacgc tatataacgc gacgtattcc ggtccggtga tgccgtggat
     1981 cgacccggtt aaggaagctg aagcctggag aattcagatc cggggtggtg cagcgacaga
     2041 atctgactgg gtgcgtgc
//
LOCUS       DAZFAH010000370         2056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFAH010000370 DAZFAH010000000
VERSION     DAZFAH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.373"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..504
                     /locus_tag="ACHJWB_004916"
     CDS             <1..504
                     /locus_tag="ACHJWB_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4316370.1"
                     /translation="DRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTL
                     FRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQPGDILLCCFGA
                     SVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHASAFTGICNDL
                     AAASACM"
     gene            501..1082
                     /locus_tag="ACHJWB_004917"
     CDS             501..1082
                     /locus_tag="ACHJWB_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316371.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIARGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(1273..1800)
                     /gene="sodC"
                     /locus_tag="ACHJWB_004918"
     CDS             complement(1273..1800)
                     /gene="sodC"
                     /locus_tag="ACHJWB_004918"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4316372.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          463 a          512 c          615 g          466 t
ORIGIN      
        1 gaccggcggc tgcagataaa aagcgcactg ccctggtggc tggtctgccg gggtgacatt
       61 cacaaattcc gctgcgtgcc gcacctgacg ggacggcgct ttgagcacgg ggtgacggac
      121 tgttacaccc tgttccggga tgcataccat ctggcgggaa ttgatatgcc ggattttgag
      181 cgtgaggatg actggtggcg caatggtcag aacctgtacc tggacaatat ggaggcgact
      241 ggtttttaca ggatttccct gccttccgca cagcctggcg atatcctgct gtgctgcttt
      301 ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact gcttcaccat
      361 ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg acgaacgcat
      421 tcagcctggc gtcaccgcca ctggcacgca tctgccttca cggggatttg caacgatttg
      481 gccgccgcct cagcctgtat gtgaacacgg cagcggaagc cattcgcgcc ctgtcgatgc
      541 agatgccggg ctttcgccgt cagatgaacg aaggctggta ccagatacgt attgccggtg
      601 atgacacggc accggaggcg gtgtatgccc gtcttcacga acagctgggt gagggaacgg
      661 tcatccacat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag attgtgctgg
      721 gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg ttatggggta
      781 cagccctgag tgccggtggt ttttctgcca ccacgatgct gttttcactg ggggccagca
      841 tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg
      901 caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg attgcccggg
      961 ggaacccgat gccggtgcct tacggtgaaa tgctggttgg ctcccgccgt atatcccagg
     1021 acatcagtac ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg cggcaggcat
     1081 aaaagcgaaa aaatcccgca gtgctcacgg acaggaactg cgggagagtt acgaagattg
     1141 agtgtaagga attattctta tgtcacgaca aaaacattaa ctcagagagg gaggatgtgc
     1201 cgttcttttc agggagaaag gatttatcgt cctgaggaat aaaggtaagg ggcccgcccc
     1261 ttacctgact gattattgaa tgatgccgca ggccattctc gcaccaccac cgcccagggg
     1321 ctccggatgg tcatgatggt tatcaccgcc agcatgaagc atgagagaac gccctttaat
     1381 ctcttttaat gagttcagtc tcggggccag gaccgggtag ttcgcttttc cgtcatgcgt
     1441 cacgaacagc gcagggaggt cgcccaggtg tccatccgga gaccaggggc caagatgttt
     1501 gccggtgttt ttcgggtcaa agtgaccgcc agccgataat gctgcgaccg gttttccgtc
     1561 tttcagtgcc ggggcgcaat ttcctttttc gtgcacatga aaaccatgaa tgccttcaga
     1621 cagagagtga agggctggtg tgaacagcag accgtagggg gtctcctgaa tggttatttt
     1681 tccaatgctg acttcttttc cgtcagcact gacaaggttc attgggactt cctgttctgc
     1741 tgcgtatccg catgatgctg ctgtcagcat ggcaatggca gcaatgattt tacatttcat
     1801 aaaaccctca ttaattccgt taacagactg agcttgctgg ttacagggta acaaacagcg
     1861 ttctgatgat atcgcgcaat agctgtgcaa tatcctatca ctgcgattaa taataccaat
     1921 tgagaggaac attatgggta aaggtgacgg cagggcgcac acgccggttg aggcaaagga
     1981 caatcttaag tccacgcaga tgatgagcgt gattgatgcc attggtgaag ggccgattga
     2041 aggtccggtg aagggg
//
LOCUS       DAZFAH010000371         2044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFAH010000371 DAZFAH010000000
VERSION     DAZFAH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.374"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..132)
                     /locus_tag="ACHJWB_004919"
     CDS             complement(<1..132)
                     /locus_tag="ACHJWB_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012305471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4316373.1"
                     /translation="MGVSACKSPPPVQSQRPEPAAWAMEKAQDLQQMLNSIITVSEVE
                     "
     gene            complement(68..373)
                     /locus_tag="ACHJWB_004920"
                     /pseudo
     CDS             complement(68..373)
                     /locus_tag="ACHJWB_004920"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            complement(370..867)
                     /gene="rrrD"
                     /locus_tag="ACHJWB_004921"
     CDS             complement(370..867)
                     /gene="rrrD"
                     /locus_tag="ACHJWB_004921"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4316374.1"
                     /translation="MPPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(867..1082)
                     /locus_tag="ACHJWB_004922"
     CDS             complement(867..1082)
                     /locus_tag="ACHJWB_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316375.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1225..1623
                     /locus_tag="ACHJWB_004923"
     CDS             1225..1623
                     /locus_tag="ACHJWB_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316376.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTRLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1703..1861)
                     /locus_tag="ACHJWB_004924"
     CDS             complement(1703..1861)
                     /locus_tag="ACHJWB_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4316377.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1947..>2044)
                     /locus_tag="ACHJWB_004925"
     CDS             complement(1947..>2044)
                     /locus_tag="ACHJWB_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316378.1"
                     /translation="STSFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          550 a          528 c          424 g          542 t
ORIGIN      
        1 ttccacctcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
       61 catcgcccat gctgctggct ccggtcgttg actttgcaca ggtggcggag acttgcaggc
      121 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcatta gcatcagcaa
      181 gctcctttgt gtatctggcg tcaagttctg ctacatcacg ttgccgcttc tgcatatcag
      241 cgatgatgga tgtggcttta tcgcgctgct ctttgtaggc gatggcgtta tcacggtaat
      301 gattaacagc ccatgaaagg cagacgatga ggcagatgac cagaacgtag ataatcgcgg
      361 ttactctgct cattgttgcc cccacaaaca gacttcacgc tcaatctcac ggcgagtcat
      421 cagccctttc cattgcttac cgccagcgta tgtccagcgc cgtagctgat cacatgcgcc
      481 tttgatatcg ccctggttta ttttgcgaag aagcgtcgat gttctgaaat tgccagcgcc
      541 cacgttgtaa acgaacgagt aaagagcgcc gcgcgttgtt tccggtatat cgactttgat
      601 gtacgggttt atttgtctgg cgaccgtggc aaggtcttta ttcaggaggg ctttgcattc
      661 tgcttcggta tacgttttac cgggcatgat gtcttttccg gtgtgtccat gacatacagt
      721 ccatacgcca acgatatctt cgtatggtat gtagctgaca ccttccaggc catcgtcacc
      781 actcggacca gtgatgagca cagacgctat ggcaacagcc ccaccaccaa tagcagcagc
      841 aacggctttt cgtaatgatg gaggcattat tcacctctcg cagccttgcg cttatcttct
      901 ttaatcttga aataaaggtt tgtcagatac gtcagcaggc caaacagcag actccccagc
      961 acacctattg ccacccactg ggacggagag actttgtcca gcagctgcag taaccagtat
     1021 cccgtcccca ccgctgacgt ggtgtatgac acacctgttg tgattttttc catctggtac
     1081 ataccccgtc tcccgttatc cggaagctga caacaataaa aagccaccag ttaattcctg
     1141 atggccctga tgcataaacg tcataatacc tgactgttat gattgacaat aatgataatg
     1201 tttatataga aaggttcccg atgtgtgtta catatcattt ctccacgggg aatatcccca
     1261 cgccagcgca gactctttta cccgttctct tctgcgctgg ctctttttta ttatgctgct
     1321 gcatttacct ctggcaccag gctttctatc tcaacacaat acgtggtact tcttgtaacc
     1381 aatatcataa cgattaatcg acatagaatt tctcccgtgt acaggaacag agttaaaaag
     1441 ccggaaccgg aatcaaatca caggatgacc atctgccagt ggcaggtcat aaaaaaaagg
     1501 ccgcgccatg cgcagccaga actcacaagg aaaatgatag aaggaaataa cattagtgat
     1561 gtacgcatgg cgcctcccgc taagttctgt aatgatcaaa cagaactcgc tacgtgccct
     1621 taaaactcga tcatttagcc cctccaagga ggattcacca tgcggttgtt ttttaataaa
     1681 cagtaaacaa aaaagtcaag aattattcat tctgttcttt catcatcggc cacagcaata
     1741 ccacaatgcc gcagaccaga gcgccatcag tcagtaccaa cattatcctg ctggtgaaat
     1801 ccatcatcac catcactaaa agcaggatca caacagcaag cagacacagt ttataaaaca
     1861 atgttcagaa aacgcattca gcatgcctaa ggttctattc ctacgaatag ccaacttgca
     1921 acttaaaata ttatttatgc agccaattaa attctggtcc ttacaatatc aacctgaaga
     1981 ttcttatctt gtgctgattg ataaatcaca aaccttttac tacctgcatt gaaagaagta
     2041 gaca
//
LOCUS       DAZFAH010000372         1979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFAH010000372 DAZFAH010000000
VERSION     DAZFAH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.375"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..218)
                     /locus_tag="ACHJWB_004926"
     CDS             complement(<1..218)
                     /locus_tag="ACHJWB_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO4316379.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISAT"
     gene            complement(388..464)
                     /locus_tag="ACHJWB_004927"
     tRNA            complement(388..464)
                     /locus_tag="ACHJWB_004927"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(428..430),aa:Arg,seq:tct)
     gene            complement(565..640)
                     /locus_tag="ACHJWB_004928"
     tRNA            complement(565..640)
                     /locus_tag="ACHJWB_004928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(605..607),aa:Ile,seq:cat)
     gene            complement(669..1382)
                     /locus_tag="ACHJWB_004929"
     CDS             complement(669..1382)
                     /locus_tag="ACHJWB_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316380.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1517..1714)
                     /locus_tag="ACHJWB_004930"
     CDS             complement(1517..1714)
                     /locus_tag="ACHJWB_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4316381.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
BASE COUNT          549 a          510 c          421 g          499 t
ORIGIN      
        1 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
       61 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      121 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      181 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacattcct ttgggtgggt
      241 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      301 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      361 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtcgaa ccactgaccg
      421 attgcttaga aggcaattgc tctgtccggc tgagctaaca acgcagaata ccgataatgg
      481 accgccatcg gggacccggc cccgcaccaa caaccctgta atggtgtcgt ctgctcttcc
      541 tgataagcta atggcggttt gtgatggtgg cccttgctgg atttgaacca gcgacctggc
      601 gattatgagt cgctcgctct caccactgag ctaaagggcc gagccaaaaa ataataatca
      661 gatgaaaatc aataatcaag cccttgcctg gatacatatc tgtctggcgg gaagccataa
      721 tagcggtgaa atacagaaat aaagtaggac ctgcttgaat aaccacattt ttctgctaca
      781 gcctgtccat atccataccg ggaacataac atatttacag caacacgcat ccgctcttcc
      841 agcaacaagc gactgaacat gcccccttca tttttcagtt ttgtctttaa caaactctca
      901 ctcatatgca actgtagagc aatcgcacca agcgtccagc ttgctgatat atctgtctga
      961 attatcgccc tgactttggc acttatgctg gataaacatc cacttaaaaa caatgacatc
     1021 cgttcatctg tttcaaacag agacaggcag gccatcataa gaaacatatc cgtggcctct
     1081 ccggaaaatc cctggctggt aattaaagcc gcagccaacg caggattgtt gggttccagc
     1141 aacaggtaaa gcggaatgtc agtcagacga gtcctcgtca gcttatgctg actttccaga
     1201 tattgactta cgacggattc gcttatatcg acaattttaa ctttgccata atgcataagg
     1261 aaaagctccc tgatgcattt ggtggccaga acaactgagc ctggcttaag tgacaacgta
     1321 tccttttcaa gaaaaatatt aattggggag caaaccatga taactgaaca gacaacagcc
     1381 attataattt tactttcatt agcaattggt tagctcaatt atagccccaa aaggtaaatt
     1441 atcatcaaca cataagcaaa ggactgacag gtgtcgcccc cccaccagcc gcccattcac
     1501 cacaaataaa aagccttcag gactgaaggc gtctgtaaca accgcactga tagtctgcca
     1561 gacccgccat aacaagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     1621 tctgcgcaat ctccccgact gtcgccggtt cggtaacgct taattcatca aacacaactc
     1681 tggcggtttc tgtcatatcc tgctgtttca gcatgtcttt ttaccctttc cggttaacgt
     1741 gacataccaa taactcttgt ctaaaaagcc agcaagataa aaagccagta ttcacgacca
     1801 ccagcgtgtt tactgtactg cacaaggttt acaggtacaa aaaaaccgct cagcggcggg
     1861 tttaagttgt gtggcgaagt aaccactctt aacacgatat aatacttttt gcgtacgcgt
     1921 taggattttt ataaactatg cgtccccatt ctcgctatct ttagtcggtc ctggaatac
//
LOCUS       DAZFAH010000373         1975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFAH010000373 DAZFAH010000000
VERSION     DAZFAH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.376"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1193)
                     /locus_tag="ACHJWB_004931"
     CDS             complement(<1..1193)
                     /locus_tag="ACHJWB_004931"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO4316382.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTDAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPECEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSTPTSFNFMGGDLWGVIDKLDYLEDLGIDGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRG"
     gene            complement(1211..>1975)
                     /locus_tag="ACHJWB_004932"
     CDS             complement(1211..>1975)
                     /locus_tag="ACHJWB_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO4316383.1"
                     /translation="VLGPVIWTIMASFKKGNNLFSSTFSGIEFTFDHYITLFTDTPYM
                     QWYLNTFILATANMLISLFVVTMTAYVFSRYRFRGKRNTLMSILVLQMFPAFLSMTAI
                     YILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGYFDAIPTSLDEAAKIDGAGHLTIF
                     IQIILPLAKPILVFVGLVSFTGPWMDFILPTLVLRSEDKMTLAIGIFSWIASNSAENY
                     TLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVKE"
BASE COUNT          647 a          456 c          335 g          537 t
ORIGIN      
        1 ccacgcaacc agggggtgcc ctcatgccat atctcaccca aaatataaca ttcaggtttg
       61 atatctttaa tcacctttct gaaatctcgc cagaaatgat gatccacttc attcgcaaca
      121 tccagacgcc aaccatcaat atcaaaattt tgcgtccaat agcgaactat actaagtaag
      181 tattcacggc actcttcatt ctcagtattt agcttgggca tttcaataac attgccaaag
      241 gtttcatagt taaagttttt aaaatcccat tcactttttg gagtatcggg ataaacagga
      301 aatttatgaa tccagaacca atcggcgtaa cgtgaattgg caccattgcg tacaacgtca
      361 agccatattg gagaatcagc tcctaaatga ttaaatactg catccaacat aatttttatt
      421 ccacgcttat gcgcttcttc aatcaaagta tggaaagcaa tattcccgcc taaatgtgga
      481 tcaacactaa aatgatcgat ggtgtcatat ttatggttgg aagctgaggt aaaaatagga
      541 caaaaataaa tcccatcaat acccagatct tccagataat cgagtttatc aataaccccc
      601 cacagatccc cacccataaa attgaatgaa gtcggtgtac tgccccacgg ttctacccct
      661 tctggggata tttcaggtct gccattacag aaacggtctg gaaaaatttg gtaccatacc
      721 gtatttttta cccaggaggg cgtatttaga acgtcaattt tattaagata agggaagcaa
      781 aaaaagttat ttaagcggct taactctcgt tcttcgcact ccggtgaact gatatcaaca
      841 caccttttct caccaaaaag aaacttctct ccctctttcc caaaaagtat aaaaccgtaa
      901 cggcaacggc gtttttgagg tttgaacact gcaaaccagt gatcatgaaa ttcggtgaca
      961 gcttcttgtt ccatctcgat ctcgttgccg ccaatccagc cgaacgcgtc tgtcccggcc
     1021 atgttaccgc cgtcaagacc accttcagcc caattatatg gatcgcctat ccagagaata
     1081 actttatcaa cttctcctct taatgtgcgt aaccgtacat gcaaagtttc gttatcatag
     1141 gcatagctat cttcactttt tgctgagtgt tttaacgaac taactgtaat cataaatact
     1201 aatccctaaa ttattcctta acggcaccgc taacaagtcc agtagttata tgcttctgtg
     1261 tgaatacaaa taacaatgtg ataggtactg caactaaaag cgccccagca gcaaaaagcg
     1321 tataattctc tgctgaatta gaagcaatcc aactaaaaat gccaatcgcc agggtcattt
     1381 tatcctcact acgaagcacc aacgttggca aaatgaagtc catccatggt ccagtgaacg
     1441 aaactaatcc aacgaaaacg agaatcggct tagccaatgg caatattatc tgaataaaaa
     1501 tagtcagatg ccctgctcca tctattttag cggcctcatc caaagaagtt ggaatagcat
     1561 caaaataacc ttttaccaac cacgtcataa agggcaatga accggttaca taaaccagaa
     1621 gcaacccagt atatgtgtct atcaaattgg cctttgatag caggatataa attgcagtca
     1681 ttgataaaaa tgcaggaaac atttgcaata caagaatact cattaacgta ttacgctttc
     1741 cacgaaatcg ataccttgaa aaaacgtagg cggtcatcgt cacaacgaac agtgaaatta
     1801 acatgttagc ggtagcaaga atgaaagtgt tcaaatacca ctgcatatat ggcgtatccg
     1861 tgaacaacgt tatgtagtgg tcaaatgtaa actcgatgcc actaaatgtt gaactaaata
     1921 gattattccc ttttttaaaa gatgccatta tcgtccatat cactggccca agcac
//
LOCUS       DAZFAH010000374         1950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFAH010000374 DAZFAH010000000
VERSION     DAZFAH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.377"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1950
                     /locus_tag="ACHJWB_004933"
                     /pseudo
     CDS             1..1950
                     /locus_tag="ACHJWB_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT          451 a          547 c          593 g          359 t
ORIGIN      
        1 ttaatcagaa cggtgaactg agggtacatg ccggcgggga agccactgca gtcacccaga
       61 acacgggcgg tgcactggtt accagtacgg ctgcaaccgt taccggcata aaccgccttg
      121 gagcattctc ggttgtggag ggtaaagctg ataatgtcgt actggaaaat ggcggacgcc
      181 tggatgtgct gaccggacac acagccacta atacccgcgt ggatgatggc ggaacgctgg
      241 atgtccgcaa cggtggtacc gccagggaaa tggcggtgta ctgctggccg attccggtgc
      301 cgctgtcagt ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga
      361 tgccctgatg ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga
      421 taccacggta aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac
      481 gctgaataac ggcgccatac ttaccctttc cgggaagacg gtgaacaatg ataccctgac
      541 catccgtgaa ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga
      601 aaaatcagga agtggcacac tcactgtcag caacaccaca ctcacccaga aaaccgtcaa
      661 cctgaatgaa ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca
      721 gcgcggtaca gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac
      781 gaatgtcact ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc
      841 ggtggtggat gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa
      901 gttcgtaccg gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct
      961 gcgtgtacgc ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag
     1021 ggcaaccgga aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc
     1081 gaccagcggt aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg
     1141 ggcctttgtc caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga
     1201 cagtgatgag agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta
     1261 tgcctccatg ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca
     1321 tcagaccggt gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct
     1381 cggtcacgat aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta
     1441 tggattcgtc cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac
     1501 cgcgggggta tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc
     1561 ccgtgccggc acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca
     1621 caacgcctcc ggactgtggg ctgacattgt ggccctggga acccgccaca gcatgaaagc
     1681 gtcaacggac aataacgact tccgcgcccg gggttggggc tggctgggtt cactggaaac
     1741 cggtctgccc ttcagtatca ctgacaacct gatgctggag ccacaactgc agtatacctg
     1801 gcagggactt tccctggatg acggtaagga caacgccggt tatgtgaagt tcgggcatgg
     1861 cagtgcacaa catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg
     1921 cgaaggcacc tcatcccgtg cccccctgcg
//
LOCUS       DAZFAH010000375         1907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFAH010000375 DAZFAH010000000
VERSION     DAZFAH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.378"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..1051)
                     /locus_tag="ACHJWB_004934"
     CDS             complement(137..1051)
                     /locus_tag="ACHJWB_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316384.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            complement(1110..1613)
                     /locus_tag="ACHJWB_004935"
     CDS             complement(1110..1613)
                     /locus_tag="ACHJWB_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316385.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1626..>1907)
                     /locus_tag="ACHJWB_004936"
     CDS             complement(1626..>1907)
                     /locus_tag="ACHJWB_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316386.1"
                     /translation="GVRVAFNGIEDAENNTLLKLDEGSNTASGLGIEILDANMRPVKL
                     NDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLVRASATFTLEFQ"
BASE COUNT          520 a          439 c          398 g          550 t
ORIGIN      
        1 ttttcaatat aaaaaataag actctggctt caattgtgcg cggattttct tacaggtgta
       61 ggataatagc cttcacaaaa taattatccg cgcaattttc gtgaatggag cgcatagact
      121 aagatcaaaa tcacaattat tcatagataa aagtgacacc aatgactgac tggacggttc
      181 cggctgcaac cttgtttcct gtttgtccat aggttgcgct taaacccaga ggtactttgc
      241 ttttatttac tgtccccagg gagacatttt ctcccgtagc cagtattttt ccattacgca
      301 tcagagagac acccactccc gatgcttttg ttgcatcagg tgctgtgtta gcaaacactt
      361 gtcggctact gtcagtggtg gcacccgaaa gataaaatga gagcttttgt tcgcttgagc
      421 aatatacgcc aaggggtatt tctgcgcttc caggaaagtc cgggagatcc acagtgacat
      481 tacgactatc aacggtgcag ccgcctgttg gcataacaac attgttattt gaaataatat
      541 tccacgtaaa attacgcgga tttccgcttc ctaatgttgc aattttatac atatgaatac
      601 gagcaatgac ttcccccgct tttatgacaa ctccaccggc cgccccaact ggggtaatat
      661 agagttttaa cggtaatggc atcggtgtct tatcaccaat atccaatacg ttggtatttg
      721 tcgtcaacgg gaaggggtag gtcacattat tccagtacag cgatccttta tatgactgta
      781 gggagcctgc gaaagctgag ccttgtacca ggtttatatg atcagtgtcg taccagccgc
      841 cgtaatcatt ccagcaactt atatgctgag acaagtctac aaccagattc tggcccggct
      901 gtattacagg gtcgagatta acataaacag aggttgtacc tgctccaata cttgacccgc
      961 catcgacatt acaactgaag gcaaaaactt tgcctgccat taatagaaga taaataccga
     1021 acaggacctt tatactgatt gttttaccca tgatatatcc taaggttaaa aattgattta
     1081 aaaagaaggc taagagaatc gaatcgacgt tattgccagg tgtagatcac attgattagc
     1141 gcctcgatcg ttccctggct tgcgtttcca ttcaccgtga tagctcttgc cttaagtgga
     1201 aactgtgcat tacgagtgat ctcatcaaca ataaccgttt tgctatcgcc attttttaac
     1261 gcagcatcct ggtcatccct cagctctatc tgaatatttt cggcagtacc ttcatttttg
     1321 taataacccg tattgtcagt tgaacctgtc acgattgccg tcactgcact tgtttcaacc
     1381 ggacaatcgg ttaatgacaa agtaatattg tgccagccag atgcagaacc aggttgttgc
     1441 agattgcgcg tataaagatc cccgagatta acgttagctt ctttggtttg aatagtgcag
     1501 ggtttagcga ctacccgacc attaacagtg atagtgacat cggcggcctg gagagttgct
     1561 gtgaataacg cgcaaaacgt agctaacagg aacctcttgt gaagtctttt catatctgac
     1621 tctgattatt gaaattcaag ggtaaaggtt gccgaagccc tcaccagtcc cggattgacg
     1681 gacttctgag ttgacttcag acgagcggag taaggcaaaa tattgttctg ttctggtacc
     1741 agtgggatcc actgcatccc ggcatgaagg tcattcagtt tcaccggacg catatttgcg
     1801 tccaatattt ctatacccaa accggaggcc gtattgcttc cttcatccag tttcaacaaa
     1861 gtattatttt ctgcatcctc aataccgtta aatgcaaccc gaacccc
//
LOCUS       DAZFAH010000376         1870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFAH010000376 DAZFAH010000000
VERSION     DAZFAH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.379"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            209..742
                     /locus_tag="ACHJWB_004937"
     CDS             209..742
                     /locus_tag="ACHJWB_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316387.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKAKCAQVNAIERNKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            902..1039
                     /locus_tag="ACHJWB_004938"
     CDS             902..1039
                     /locus_tag="ACHJWB_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316388.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            1041..1508
                     /locus_tag="ACHJWB_004939"
     CDS             1041..1508
                     /locus_tag="ACHJWB_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4316389.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     EQANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPATSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
BASE COUNT          471 a          415 c          515 g          469 t
ORIGIN      
        1 gggatatctg cagatagctg ctgagattga agcgctggcc agtggtgcgg acaaggatgc
       61 agttgtgcgg gaggctcttc ttcgtgattt tgataatccg ccctttaaaa aagtgccggc
      121 ttactggttt catccaggac tgacttatct taaaggacgt atataagctg gctcgttatc
      181 tgttgccgat aaatcctgat aaatatccat gaacaccaaa atcaaatacg gcctgtcggc
      241 tgccgttctg gcgctgattg ccgctggtgc gcctgcgcct gacattctcg accagtttct
      301 ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcgggga tctggaccat
      361 ctgccgtggt gccatcatgg tggatggtaa acctgttgtt ccaggcatga agttgtcgaa
      421 ggcaaaatgc gctcaggtta acgccattga gcgtaataag gcgctggcat gggtggagaa
      481 aaacatcaaa gtgccattga ccgaaccaca gaaagcgggg attgcgtcat tctgtccgta
      541 caacattggc cccggtaagt gtttcccgtc gacgttttat aaacgaatta atgcaggcga
      601 tcgcaggggg gcgtgtgagg cgattcgctg gtggattaag gacggtggca gagactgccg
      661 tattcgctca aacaactgtt acggtcaggt atcccgtcgc gaccaggaga gtgcgctggc
      721 gtgctgggga attgacagat aagcagaata ttttgctgaa aaatgcggtt tgctcacacg
      781 gacggataac acgaaatcct gcgaactgac aaaaactaag tgaataaaag taaaaacccc
      841 gtttgttggc tgcaagcggg gttttgtgtt tcctgactct ggaaaagtca aaggagaaag
      901 tgtgtttgat tttagcaaac tgattcggga gattcgagtg atggctgaaa aattatccac
      961 ctggaagttc attcttatct ggctggtgtt tgtgattatg gcctccggtt atttcatcgg
     1021 tcagatacgc tggtggtgaa atgaaccgcg tactgtgcgt ggtcatcatt gccctgctgg
     1081 tggcctgtgg tgcgcttagt ctggggctga atcattaccg tgataacgcc ataacctaca
     1141 aagagcagcg cgataaaaaa gtcagtgagc tggagcaggc aaatgcaacc attactgata
     1201 tgcagcagcg ccagcgtgat gttgctgcac ttgatgccag atactcgagg gaattagccg
     1261 atgcgagagc tgaaaatgaa actcttcgcg ctgacgttgc cgctggtcgt aagcgcctgc
     1321 ggatcaacgc cacctgtcca ggctccgtgc gtgaagcccc cgccacctcc ggcgtggata
     1381 atgcaaccgg cccccaactg gcagacaccg ttacacggga ttatttcacc ctcagagagc
     1441 ggctgatgac gatgcacaag caactggaag gggcacagga ctatatccgc actcagtgcc
     1501 tgaaataagt tttgttgatg cgccgtatcg tcgctgtatt ccctcattaa cagagaccgc
     1561 agcccgacag ggagactcct ctgcgcgagt gtgcggggat aatcaaaaac gatacacacc
     1621 ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca tagtcgcctg tccggtgcga
     1681 tggtggaaga agccggatgt ttatcactat taattgatga cacagaaatg gattcattga
     1741 atttcagcac gtttttgtat tcgtgttatt gaacatctgt ttattttact tttaacatat
     1801 tgataataaa aagagctgta aatctttaga tgagtcgatt ttgtccgggg aagttcaaat
     1861 ggattttatg
//
LOCUS       DAZFAH010000377         1868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFAH010000377 DAZFAH010000000
VERSION     DAZFAH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.380"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..170
                     /locus_tag="ACHJWB_004940"
     CDS             <1..170
                     /locus_tag="ACHJWB_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4316390.1"
                     /translation="GGMGTKSHDIFTLPLCREHHNELHADPLVFEEKHGSQVDLIFRF
                     LDHAFATGVLG"
     gene            183..557
                     /locus_tag="ACHJWB_004941"
     CDS             183..557
                     /locus_tag="ACHJWB_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4316391.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDELTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            554..1375
                     /locus_tag="ACHJWB_004942"
     CDS             554..1375
                     /locus_tag="ACHJWB_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4316392.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     VFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1613..1868
                     /locus_tag="ACHJWB_004943"
                     /pseudo
     CDS             1613..1868
                     /locus_tag="ACHJWB_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT          489 a          374 c          535 g          470 t
ORIGIN      
        1 agggcggaat gggaacaaaa tcccacgata ttttcacgct accgctgtgt cgggagcatc
       61 acaacgagct tcatgcggat ccgctggtgt tcgaagaaaa gcatggttcc caggttgatt
      121 taatttttcg ttttcttgat cacgcctttg caaccggcgt gctcgggtaa aagaggttac
      181 tgatgcgtat agagtttgtt ttgctttacc cgccgacggt gaacacctac tggcgacgtc
      241 gtggcagcac atattttgta tcaaaagccg gtgagcgtta tcgccgggct gtggcgctta
      301 ttgttcgcca gcagcggctg aaattaagcc tgtccggaag gctggcgata aagattattg
      361 ccgagccacc ggataagcgc cgccgtgacc tggacaatat tctgaaagcg ccgctggatg
      421 agctgacgca tgcggggttg ctaatggacg atgagcagtt tgatgaaatc aatatcgttc
      481 gtgctcagcc agtatctggt ggacgtctgg gggtgaagat ttaccccata atgcttgaag
      541 ggcaggtcaa aaaatgaaac tggaagattt accgaaatac tactccccaa aatcccccgg
      601 cctgactgat gcatcggcct caacgtcgaa agatacgctg agtatcactg atgtgatggc
      661 cgcgcagggc atgacacaga attgggctga gatggggttt tctgcgttcc ttgggaaaat
      721 gggcattagt atgaatgaca gagagcgggc aacagaattg ctgacagaat atgcactcag
      781 tcggtgtgat cgcgtggcgg cgttaagaaa actcccggca gaaataaaac cggcagtgat
      841 gcgtattatg gcttcgtatg tgtttgaaga ttatgcccgt agcgcggcga gcaaaaaaca
      901 gtgcccctgt tgtcacggaa aaaaatttat tgaaagcgag gtttttacaa acaagatcca
      961 gtatccggat ggtaagccgc cagtgtgggc aaagtgcaca aaaggcgtgt atccgtctta
     1021 ctgggaggaa tggaaaaaag tcagggaggt ggtaaaagtt gcctgtccgg agtgtggagg
     1081 gaagggggag gtttccaccg cctgtaaaga ttgtcgtggg cgcggtgttg ccattcatcg
     1141 tgaagagtcg gtaaaacgtg gtatgcctgt tatcagagac tgccagcgtt gtggtggtcg
     1201 tggctatgaa agattacctt caacggaggc atttaatgcc atatgtaatg taaccgatgc
     1261 catatctctt gatacatgga aaaaaacagt taaacgtttt tacgatacgc tggtggtgca
     1321 gtttgatatt gaagaagcat gggcagaaca acaactgaaa aaggtgacca gatagctttg
     1381 ttgatttttc ccgaatctgt ggtaaatttg ccctaacgat gggcgtttta tgcctgatgt
     1441 tagaagattt tttacacccg tcgccaggcg ggttttttta tgactgaaat cacgccagta
     1501 cagtaaacgc gctggtggtt gtgaataccg gtctttcagc ttgctggctt tttcgacaag
     1561 agttattggt gtgtcacgtt aaccggaaaa aggaaagttt gagaaacgcg atctggcaca
     1621 ggcggtgatt aatgctgcct acttggtggc ctgtgcagat ggtgaatgtg aggcttccta
     1681 gaaagcgaag atcgaacagg tactgcgtaa tcagcctgcg ctgtccgcgt ttacgtcaga
     1741 aattaatgcg attagcgcaa ccattatcgg tcagctggat acgaacttta aaactggtcg
     1801 tcgagcggca ttgcgtgaaa ttgaagatgt gaaacacgat acgcgtgaag cggaagatgt
     1861 gctggatg
//
LOCUS       DAZFAH010000378         1819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFAH010000378 DAZFAH010000000
VERSION     DAZFAH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.381"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..101)
                     /locus_tag="ACHJWB_004944"
     CDS             complement(<1..101)
                     /locus_tag="ACHJWB_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316393.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAAL"
     gene            complement(92..1174)
                     /locus_tag="ACHJWB_004945"
     CDS             complement(92..1174)
                     /locus_tag="ACHJWB_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4316394.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(1174..1611)
                     /locus_tag="ACHJWB_004946"
     CDS             complement(1174..1611)
                     /locus_tag="ACHJWB_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4316395.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(1604..>1819)
                     /locus_tag="ACHJWB_004947"
     CDS             complement(1604..>1819)
                     /locus_tag="ACHJWB_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4316396.1"
                     /translation="GVQVDTPEAHFTGNITVDKNLHVKGNVSIDGTGRSQGAFTMSEA
                     VIAGITYSGHVHHDNGEGSKTGGPENG"
BASE COUNT          386 a          566 c          465 g          402 t
ORIGIN      
        1 cacaaagccg caagaacgga tgacggccgc ttatcccatg ccagcccgtc aggcagcaac
       61 tgcaacaggg cgcgctgatg cggggtcagg gttacagcca tgtgatttcc cccacggtca
      121 gcaactctgt atctccggcc atcacggact tcagcggact gcgaacttca aaatcctcca
      181 gtcctttcac ccctgcaaca gcccgccaga atgatgaagg caatacaagt ccacccggcc
      241 gcgattcgtt ataaagcagg tccgttaatg cctgttttac ggcagcctga ttttccggcg
      301 ttttgggtac aatcctgatc tcaaacgcca ccggcttatt ggtcagctta aacacgctga
      361 ttgtcggccc aagtggctga ccaacgggct ggcccgtcgc cggatcatca tgactgcgga
      421 tataatccgc cacccgcttc acatcacctt ctccggggaa aatgtcaggg ttattatcct
      481 gaacaaaagt cacaccaaca ctacccgcct caggccattc aggcttacac catgcccgcg
      541 tcacgcccgg tacttcccgt gcccagcgtt caaaatcata ctgtgttccc cctgatggcg
      601 ggttctgtac ccggaatacc agcctggaca gcaactccgg caccgtttcc acatccgcac
      661 caccggtgag tcccgtgccg gtaaccgtgg ctgtctggtt gatgcccgcc tgtggcgtga
      721 taaaggacag gcgggttccg gtcggggtat ttccggcgcg ccccgcactt tccgcctcca
      781 cattaacatt cagtgttccg gcttttccgg tcgcagagcc ggtgatgcgg tacacaacac
      841 catcgcttcg ctgaaggagt acgccttccg tgatccctgc atccgtggtc agcatcagtt
      901 gtaccggtcc gtcagcgcgg gaagccggtt tacggatgac accccagaat gcgcagtgtt
      961 tcaggagttc ggcttcatca gcttctgtcg gaattatctg ccgggagatc caggccagat
     1021 gttcatgctc ctgtgctgac agacccgcca gtgcatacgc aatggcattc agggtggttt
     1081 cattcacgcc cggctgcgaa ccgggcaggc gctggctgat atcctgttgt gtctgtgtga
     1141 tcagttgcgc cagtggtaag ggctgataag gcatcattcc cccttcatat cagcataaaa
     1201 aatcatcgga tgtctgcttc cgtccggcag ggtgattttc acggaaagcg aaaggcgatc
     1261 atgccccaca cgcatggcat gacataccac ggatgtggca gcaccgcttt tatgcagcca
     1321 cgccagcgct tcatcagcat aggcctgtgc acggctgacc acggaggaca gcgtcttttc
     1381 acggcttaac agccagagac gggagccaat ggggcgctcc cggaaactgt ctccccacca
     1441 tccccgacgg tcacgggtgc cgtcagggat ttcatcggaa tccagcgccc gcctgtccgt
     1501 aaacagtgaa ataatgaccg cagtcagcag gctgttatcc gtcagaagat ctgcgccgtt
     1561 cagttgcagg gaaccacaac cctgatccca gacgacagca atatcagcca ttctctggtc
     1621 ctcccgtttt gctgccttca ccgttatcgt gatgcacatg acctgaatag gtgatcccgg
     1681 caataaccgc ttcggacatc gtgaacgccc cctgtgatct tccggtgccg tcaatggaca
     1741 cattgccttt gacatgcagg ttcttatcca ctgtgatatt accggtaaag tgagcctccg
     1801 gtgtatccac ctgaacacc
//
LOCUS       DAZFAH010000379         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFAH010000379 DAZFAH010000000
VERSION     DAZFAH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.382"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..243)
                     /locus_tag="ACHJWB_004948"
     CDS             complement(<1..243)
                     /locus_tag="ACHJWB_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316397.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLM"
     gene            complement(255..407)
                     /locus_tag="ACHJWB_004949"
     CDS             complement(255..407)
                     /locus_tag="ACHJWB_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4316398.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            complement(680..1396)
                     /locus_tag="ACHJWB_004950"
     CDS             complement(680..1396)
                     /locus_tag="ACHJWB_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4316399.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKADDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTEIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1446..1661
                     /locus_tag="ACHJWB_004951"
     CDS             1446..1661
                     /locus_tag="ACHJWB_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4316400.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            1658..>1815
                     /locus_tag="ACHJWB_004952"
     CDS             1658..>1815
                     /locus_tag="ACHJWB_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4316401.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALAR"
BASE COUNT          484 a          396 c          411 g          524 t
ORIGIN      
        1 cattaacaag ccaaatcctt tattaatgat gtccattaat tccaggaagt atttttcatg
       61 taaatcctgg ttatcagaga gctgcttctc ttcgtacaga ccgataaagg cacgacgcac
      121 gttaccggat atagtatcga tggtttcttt ttctacggta ctcaggtcaa gagtcgccag
      181 ttgagagcga accacattcg atgccatttc ctggaatggt actggtaaat ctttaaattc
      241 catcgtcaac ctcatcagtc agtgtttctg gttaaccagc gacgcgcgcc agcttcagtt
      301 ttaaacgttt tgcttctggt atacgtcatc gcggtaaacg tgccgtcctg gttgggaaac
      361 acgccgtaca ccagagattc gttgttgcca agatcgatag tatccatgct gacctcattt
      421 ccccttaacg ctggggtagc ggaactaaaa acctgctgcg ctgttataca aagtgttccc
      481 gccgtcatgt tcatacgcct cgggctggct acttaccccc tgaccactgc ttggtaactc
      541 gaagtattgc ccggcgttct gtggggcggg gtgggttggt atgttgttaa ggtaacaaga
      601 gttacctttc gagtcaatac aatgttgcaa aaggtacatt tgagggcatg aaaaacccgc
      661 aatgaatgcg ggttctgact cagtctaagt attgatgtat ttgtgaaact ttacctttaa
      721 tggtgtaacc accattcagt tcgatgggtt tgtaaagcgg attcagtgac aacagataga
      781 tgtttggtcc gtcaatcgca acttttttta gtgttacgtt tggcgttcct tccaattgga
      841 ttaagattat ttttcccacc agttctctaa tgttacttga gcatggtgtg atcagcacgg
      901 tagatccgtc ggggatggtt gggaggccgt tagagtttgt catcgcatct ccctcaacat
      961 gcaataaaaa agagttttca gcggtttttg tcatgacatc aacccaattc ttaataccag
     1021 gaatctcggt tactggacaa ctcatatccc aataaccagc ctgttcccac gttaaaacgg
     1081 gcaaccgggc gatgttgtca ctaatgtaag ggtactgatt cagacgcaga tcatcggctt
     1141 tatcgtgacc gtcctttcca taaagaatcc attcaggaga tttggaaagc aattttgaca
     1201 gtagatacaa attctcaccg tcaggttttg aagagccatt ttcccatttt gttacggata
     1261 cacgagatat gccgattgct ttcgcaacct gctgttgggt taatccaacg tcttttcgac
     1321 gattccgaat acgttcgctg atagtgtttt tcatgtaacc aatgttacta ccaagtgatg
     1381 ttgctatggt tgacattgtt atgtaactat tgttaccctt ctactcgaaa taacaggaga
     1441 gttttatgtt caaagatgat gttctgcgct atttcaaaaa aaagcgacta gtagctgagg
     1501 ctcttggaat ttcacatgtg gctgttgtgc ggtggaaagc agttattccc aaacttcgcg
     1561 caatggaact ggatgaaatt actaacggtg aattgaaata caacccagaa ctttacaaga
     1621 agcaggatag cacctcaaac gaaggaaaga atgattcatg aaaatcaagc atgaacacat
     1681 ccgcatggcg atgaatgtct gggcgcatcc ggacggcgaa aaagtaccgg ctgcgaaaat
     1741 taccaaagcg tatttcgagc tgggaatgac gttcccggaa ctgtatgacg acagccatcc
     1801 ggaagcccta gcccg
//
LOCUS       DAZFAH010000380         1792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFAH010000380 DAZFAH010000000
VERSION     DAZFAH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.383"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..381
                     /gene="bssS"
                     /locus_tag="ACHJWB_004953"
     CDS             127..381
                     /gene="bssS"
                     /locus_tag="ACHJWB_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4316402.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            496..1614
                     /gene="solA"
                     /locus_tag="ACHJWB_004954"
     CDS             496..1614
                     /gene="solA"
                     /locus_tag="ACHJWB_004954"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4316403.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1662..1775
                     /locus_tag="ACHJWB_004955"
     CDS             1662..1775
                     /locus_tag="ACHJWB_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4316404.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          454 a          434 c          435 g          469 t
ORIGIN      
        1 tttatctgtt cccctcttta aaaattcggt gaataccctt acttattggt atactgaagt
       61 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
      121 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
      181 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
      241 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
      301 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
      361 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
      421 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
      481 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
      541 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
      601 ccaccgcatc aacacggcag ccaccatggc gatacgcgat taattcgcca tgcttatggt
      661 gaaggcgaaa agtatgtccc gctggtcctc cgcgcgcaaa cgctgtggga tgaactttcc
      721 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct
      781 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
      841 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
      901 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
      961 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
     1021 cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa
     1081 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
     1141 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
     1201 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
     1261 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
     1321 gttccgtttg cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta
     1381 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
     1441 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
     1501 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
     1561 gacaaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
     1621 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
     1681 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
     1741 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca ta
//
LOCUS       DAZFAH010000381         1775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFAH010000381 DAZFAH010000000
VERSION     DAZFAH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.384"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHJWB_004956"
     CDS             complement(<1..71)
                     /locus_tag="ACHJWB_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316405.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRA"
     gene            complement(74..355)
                     /locus_tag="ACHJWB_004957"
     CDS             complement(74..355)
                     /locus_tag="ACHJWB_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4316406.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(348..674)
                     /locus_tag="ACHJWB_004958"
     CDS             complement(348..674)
                     /locus_tag="ACHJWB_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO4316407.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(762..>1775)
                     /locus_tag="ACHJWB_004959"
     CDS             complement(762..>1775)
                     /locus_tag="ACHJWB_004959"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4316408.1"
                     /translation="LARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMV
                     GLALTHSTSDFGNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFG
                     SLRQVREGAEYKYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAA
                     KATIGDLVYKVLTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRA
                     LNIRPAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPK
                     TWYLAAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRS
                     SVA"
BASE COUNT          378 a          572 c          433 g          392 t
ORIGIN      
        1 gcccggggaa cggcacggcg atcaattgca tccagatttt tcatggcctg cgccagacct
       61 tttattgcca tactcattcc tgttcgacaa aaatccgggg tttaccgttg tacgtgtcat
      121 aacgggtcac cgtcagtgtg cgccccgcaa acacaacaac atcatgacgg gccggacggt
      181 accgggctga aaacaccacc agtgacaact ggctgcccga aagcgccccc atctccgcgg
      241 actcttcctc cggcatcacg tcgtacacga cgccgttaat ctccgcctgt ttgcccatca
      301 cccgaacggt cgccacgtcc atccggcaac acattcgcgt aaacagatca gacattgatt
      361 tttaccgcca cagtggcgct gtttgcaggg gcattttccc aggctacccc cgctgccacc
      421 gcaccgtctg cagccagctg cacaaccccg tccttcagat aaaccaccgc gccggactga
      481 atgtcgtcag cagactgttt gggcagcagg aacacgcctt cggcaaaacc gtcaccggcc
      541 tcaccggcag gaatatcggt aatggccacg gccaccatac tgccgaccac caccgcagca
      601 ccgctcagga tggtctgatc tccggcattc accagttcaa tggtggtacc gtcctgtaca
      661 aaatttttcg ccataatgct gtttctccgg acagcccctg tggggctgtt tttcaggcat
      721 aaaaaaagcc ctttcgggca gtgattgtga taacgcggtt atcaggccac cgacgaacgc
      781 accagcccgc gccagtcaag tggtgccact ccggcatcaa tacggatttt tgtggcaatg
      841 ccgtcagtgg tgaaaccttc ctgctgatca atgtatggcg tgtccacacc atccagccag
      901 gccacttcaa tggtgtcagt gccctgtgcc gccgccagat accaggtttt cgggtctgcc
      961 gcatcaagac gcgcttctgc aatcacctca gcaaagttct ggatagggtt aatgacaccg
     1021 gcgtttgcat ccgccccttt cacactggcc gatttgatgg tctggttcgc caccgtctcc
     1081 agtgccaccg gtaccagcat aaaggccgga cggatattca gggcacgatc gccttctttc
     1141 tgcaggcgca tcatctgacg ggccgcatcc agtccggaaa cggagatccc cccggtggca
     1201 atatttttgt gatcggcatg gaacagcgcc ttaccgtcgg acagtttcgg gttatccgtc
     1261 agcaccttgt agaccaggtc accaatcgtt gccttcgccg cacgccccat cttcatcggc
     1321 acgtccacca gcatattcag atcatcattg ataatggcct ggcgggtgat ggagaaaatc
     1381 tccccgtaag tggccagtgc aatggtctcc ttgcgatctg aggtggtgat gtatttatac
     1441 tccgccccct cacgaacctg gcgcagagaa ccaaaaccgc ccatccccac gcgatacgct
     1501 gttttgaagt ctgacaggcg tcccttacgg gtccacttct ggaaggtttc ttctgattcc
     1561 tcccagccct ggatcagccc cttgttcgac acatccagca ggatattgcc aaaatcagag
     1621 gtgctgtgcg tcagcgccag cccgaccatc tgcatggggt tataactggc caccccaata
     1681 ccgcgctccg tcagtgacat gcgagcccat tcacgcaggg tcatcccgtt ataggcgtta
     1741 tccttctcga cattttcaaa tccggcacgg gccag
//
LOCUS       DAZFAH010000382         1769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFAH010000382 DAZFAH010000000
VERSION     DAZFAH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.385"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..228
                     /locus_tag="ACHJWB_004960"
     CDS             <1..228
                     /locus_tag="ACHJWB_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4316409.1"
                     /translation="VLRSEEVLSALKQKLRHNLEARLDAEVDAILDELLGVQAEPPTE
                     AGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            305..520
                     /locus_tag="ACHJWB_004961"
     CDS             305..520
                     /locus_tag="ACHJWB_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316410.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            525..1058
                     /locus_tag="ACHJWB_004962"
     CDS             525..1058
                     /locus_tag="ACHJWB_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316411.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1357..1704
                     /locus_tag="ACHJWB_004963"
                     /pseudo
     CDS             1357..1704
                     /locus_tag="ACHJWB_004963"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1667..1762
                     /locus_tag="ACHJWB_004964"
     CDS             1667..1762
                     /locus_tag="ACHJWB_004964"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4316412.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSENG"
BASE COUNT          436 a          404 c          526 g          403 t
ORIGIN      
        1 gtcctgcgct ctgaagaagt cctgagcgca ctgaaacaaa aacttcgcca taacctggaa
       61 gcgcgtcttg atgcagaggt tgatgccatt ctggatgagc tgcttggtgt acaggcagag
      121 ccaccgactg aagcgggaga taccaccgca gagagcggtg aagttcagcc tgaatcaccg
      181 gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga tgctgtaacg gggagtcagg
      241 gccatcagta aacagctgct ggcctttttc atgttgtgag cttccggatt gcgggagacg
      301 gggtatgtac cagatggaaa aaatcacaac aggtgtgtca tacaccacgt cagcggtggg
      361 aacgggctac tggttcctgc agttgctgga cagggtttcc ccgtctcagt gggcggcaat
      421 aggcgtgctg gggagtctgc tgtttggtct gctgacgtac ctgacgaacc tgtatttcaa
      481 gattaaagaa gaccggcgta aggcggcgcg gggagagtaa gctgatgagc aggaaattcc
      541 gctatggttt atcagcggta gttctggcgc tgattgccgc aggtgcttct gcgcctgaaa
      601 tcctcgacca gtttctggat gaaaaggaag gtaaccacac cacggcatac cgtgatggtg
      661 cgggtatctg gaccatctgc cgcggtgcca tcctggtgga tggtaaacct gtcgttccgg
      721 gcatgaagtt gtcgaaggaa aaatgcgacc gggttaacgc cattgagcgt gataaggcgc
      781 tggcatgggt ggagaaaaac atcaaagtgc cgctgaccga accccagaaa gcggggatcg
      841 cgtcattctg tccgtacaac attggtcccg gtaagtgttt cccgtcgacg ttttacagac
      901 gaattaatgc aggtgatcga aaaggtgcct gcgaagcgat tcgctggtgg attaaggacg
      961 gtggcagaga ctgccgtatt cgttcaaaca actgttacgg tcaggtatcc cgtcgtgacc
     1021 aggagagcgc gctggcgtgc tggggaatcg acagataaga agaatatttt gctgaaaaat
     1081 gaggtttgct cacacggacg gataacacga aatcctgcga actgacaaaa actaagtgaa
     1141 taaaagtaaa aaccccgttt gttggctgca agcggggttt tgtgtttcct gactccggaa
     1201 aagtcaaagg agaaagtgtg tttgatttta gcaaactgat tcgggagatt tgagtgatgg
     1261 ctgaaaaatt atctacctgg aagttcattc ttatctggct ggtgtttgtg attatggtct
     1321 ccggttattt catcggtcag atacgctggt ggtgaaatga accgcgtact gtgtgtggtc
     1381 atcattgccc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca ttaccgtgat
     1441 aacgccatca cctacaaagc gcagcgcgat aaaaaagcca gagagctgga gctggcaaac
     1501 gcaaccatta ctgatatgca ggtgcgccag cgcgatgttg ctgcgctcga tgcaaaatac
     1561 tcaagggaat tagctgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct
     1621 ggtcgtaagc gcctgcggat caacgccacc tgctccggta ccgtgcgtga agccaccggc
     1681 acctccggcg tggataatgc aaccggcccc cgactggcag acaccgctga acgggattat
     1741 ttcatcctca gagaacggtt gatgacaat
//
LOCUS       DAZFAH010000383         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFAH010000383 DAZFAH010000000
VERSION     DAZFAH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.386"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..349
                     /locus_tag="ACHJWB_004965"
     CDS             53..349
                     /locus_tag="ACHJWB_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO4316413.1"
                     /translation="MEAANLSPSGAVLPLAATINGNNPVDEKRGVMQSEGGTSRSVRI
                     LGGVLIGAGVLAAIGTGIAAMCVDDPSQRLDLGIAAGVLGGVTTVAAGLAMKYA"
     gene            430..807
                     /gene="sctI"
                     /locus_tag="ACHJWB_004966"
     CDS             430..807
                     /gene="sctI"
                     /locus_tag="ACHJWB_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4316414.1"
                     /translation="MNNINQSENINIQLNKASQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            810..1382
                     /gene="escJ"
                     /locus_tag="ACHJWB_004967"
     CDS             810..1382
                     /gene="escJ"
                     /locus_tag="ACHJWB_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO4316415.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1388..>1767
                     /gene="sepD"
                     /locus_tag="ACHJWB_004968"
     CDS             1388..>1767
                     /gene="sepD"
                     /locus_tag="ACHJWB_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO4316416.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSKEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFD"
BASE COUNT          596 a          338 c          357 g          476 t
ORIGIN      
        1 tggttattta taaccaggta aatgtctaaa tcagacaaaa ggagaaagat caatggaagc
       61 agcaaattta agcccttctg gtgcagtatt accactggca gccacaatca atggaaataa
      121 tccagtggat gagaagagag gggtgatgca gtcagaaggt ggaacaagtc gcagcgttag
      181 aatcctgggt ggagttttaa taggtgcagg tgttctagcc gctatcggta ctggtatcgc
      241 agcaatgtgt gttgacgacc cttcacaaag attagactta ggaatcgcag ccggtgtatt
      301 aggtggcgta actactgttg ccgccggatt agcgatgaaa tatgcctaat catcgcatcg
      361 cataaaaata gagaggtaat ggatgcatta tgctattgcc tctctcatga aaagagacta
      421 acagtaaata tgaataatat taatcaatcg gaaaacatta atattcagtt aaataaagca
      481 tcccaaacaa atgtcgttga tgagcatatc ccgttagcat caaccccctc tgcagccgga
      541 gcggctcagt ttcttgatca attactccct aaaacggcgg gagtgtcttc cccagaacaa
      601 gtgttgattg aagaaattaa aaagcgacat cttgcaacaa tgaacaccga tctcagtttc
      661 gatgcactat ctgcaggtgg gctctcgccc gaagatgtac tcactttaca aaagaatgtg
      721 ctcaacgcaa acgtcaatgt tgatgtcgta tctaagttag caagcctcct ttcaacatcg
      781 gttacaaaat tagtttcgat gcaataaaaa tgaaaaaaca cattaaaaac ctttttttat
      841 tggctgctat ttgtttgaca gtggcatgta aagagcaatt gtatacagga ttaacagaaa
      901 aagaagctaa tcagatgcaa gcactgttgc tatccaatga tgtcaatgtt tccaaagaaa
      961 tggacaaaag tggaaatatg acactcagtg tggaaaaaga ggattttgtt cgcgcaataa
     1021 cgattttaaa taataacggc ttcccgaaaa agaaatttgc agatatcgag gttattttcc
     1081 cacccagcca gttagtggca tcgccatcac aagaaaatgc aaaaatcaac tatctcaaag
     1141 aacaagacat cgaaaggttg ctaagtaaaa tacccggtgt tatcgattgc agcgtttcat
     1201 taaatgttaa caataacgaa agtcaaccat cctcagcagc cgttttagtc atcagctcgc
     1261 cagaggttaa cttagcacca tcggtcattc agataaaaaa cctggttaag aatagtgtcg
     1321 acgatctcaa attagaaaat attagtgttg ttatcaagtc atcctcagga caggacgggt
     1381 aatatgtatg aacaataata atggcatagc aaagaatgat tgtgactggc taacggccct
     1441 tgattttgta aaggatgtca acggttcacc aacgcatctc actttttata tctaccaaaa
     1501 aaatgctttc ttgcacgatt ttggaaacta ctgggttcta tatatcgagc ttagtggtga
     1561 ttttcgtcag gttccaacag atacatttat cagactctgc aatatcctgg ccgttagtaa
     1621 agaatataaa caaatgggta ttttcctgtc taataagaag tggtatcttt gccaaatttt
     1681 tcataaagat aataatcatc gcgcaaacat gtcaaaagca ataatgcaac atactctcgc
     1741 ctcgttgctc gacaagcaat ttgataa
//
LOCUS       DAZFAH010000384         1759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFAH010000384 DAZFAH010000000
VERSION     DAZFAH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.387"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..287
                     /locus_tag="ACHJWB_004969"
     CDS             <1..287
                     /locus_tag="ACHJWB_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO4316417.1"
                     /translation="TTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPKYIQFSD
                     HIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            630..1160
                     /locus_tag="ACHJWB_004970"
     CDS             630..1160
                     /locus_tag="ACHJWB_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4316418.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSHLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          560 a          371 c          333 g          495 t
ORIGIN      
        1 aaacaacatc gtgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca
       61 agaaaggcgt tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata
      121 tacaatttag cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg
      181 gtaatgattc acagcaatct ttattaaatg aaatggaaaa ctggccaacg tattatccat
      241 atcagttgag tagcgaagaa gtggtcgagg aaatgatgtc tcattgagta ttctcatgat
      301 aacgcctcga tgccgcttta gtaagttatc ataactgcca ctggtccatc cacaaacgcc
      361 actgaacgca agctagctac agacacgctc atcactatga cgtgtctgta tattaataag
      421 ctaacccgca ttgagttaac caataacgga ttccatacac aatacggcca acagagaaag
      481 atacctgtgc tcacgccatt gcttatattg gctggttaca atatgcacta tcaatttttt
      541 aaataacaaa ttattaaaac cacatctgta taaggaattt ttaaggttcg tgggatagct
      601 tgactgtgaa aatcacagga gctacaaaaa tgaaccgatt ctcaaaaact caaatttatt
      661 tacattggat aacgctgctt ttcgttgcaa taacctatgc cgcgatggaa ctccgtggct
      721 ggtttcctaa aggtagtagt acttatctgc tgatgcgaga aacacattac aatgcgggta
      781 tattcgtttg ggtgttaatg ttttcacacc tgattataaa acaccgttat agtgatcctt
      841 ctattgtgcc accgccacct gcctggcaaa tgaaagcggc ttcgctaatg cacatcatgc
      901 tttatataac cttccttgca cttcctctgc tggggattgc tttgatggct tacagtggaa
      961 aatcgtggag tttccttggt ttcaatgtat ctccctttgt taccccaaac agcgaaatta
     1021 aagcactgat aaaaaatatt cacgaaacct gggcaaatat aggctacttt ttaatcgcag
     1081 ctcacgctgg cgcagcactc tttcatcact acattcagaa agataatact ctgttacgaa
     1141 tgatgcctcg ccgcaaataa gtcgatcccc tgacaaggaa atgtgaacca tggacatcaa
     1201 cgcgtcataa atgtttacgc aagtgcacaa tgcacccttt ccactatcaa caaggaggag
     1261 cagaaactgt gtaaatcgta caatggtaag acttatcaaa aatacaggtt aattttggta
     1321 tttaaacctt ttttacttgt tagtatgata caaaggcttt caaaaaagct gggaaattca
     1381 acaactcttg gaaattatgg actttgtccc gcggctttaa tctgacggcc gcgttccttt
     1441 tttgactcaa ttattgaccc cttctctacg caacttcagt tcccaccacc aactttgcgg
     1501 cagtttcata ggatgaatgt ctaaaagaat aatggtgagc gataagaaaa cgactgaata
     1561 actgcacatt ttcgctcgaa agcttcctgt cagatccata gcgaatcaag tgctgaatgt
     1621 cacagtatcg aacagaaaac agtgacgatc taacccttca agaatattct acgattgttc
     1681 tgtttaggaa aagcaaggcg ggaagtcggg agataaatca ttgataaagt ggcggagaga
     1741 gggggatttg aacccccgg
//
LOCUS       DAZFAH010000385         1721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFAH010000385 DAZFAH010000000
VERSION     DAZFAH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.388"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1721
                     /locus_tag="ACHJWB_004971"
     CDS             <1..>1721
                     /locus_tag="ACHJWB_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFO4316419.1"
                     /translation="NKAAQRSITAPDRIGEGKSWVYQRDTMIERGDNSGVHQYGPAEH
                     FTHIISDKPSPKDEYIAYAINIPDYELAADVYNINVTSPSEQQETFKILINPEHLRQT
                     LERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVT
                     TFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYT
                     FNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIH
                     PEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREM
                     RKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYL
                     VTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSE
                     RRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTE
                     IKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGIFATKNVLAFSR
                     GDSVNIN"
BASE COUNT          596 a          337 c          329 g          459 t
ORIGIN      
        1 gaataaagca gcacagcgta gcattacagc gccagacagg ataggagaag gaaaatcatg
       61 ggtttatcaa agagatacaa tgattgaaag aggtgataac agcggtgttc atcagtatgg
      121 tcctgctgaa catttcaccc acattatatc tgacaaaccg tccccaaaag atgagtatat
      181 tgcatatgct attaacattc ctgactatga gctggcagcc gatgtatata atattaacgt
      241 gacgtcacct tccgaacagc aagaaacatt taaaatacta atcaatccag aacatctacg
      301 gcaaacactt gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg aaatcataac
      361 ccccaaaaaa cctggcgaag cgattcttca tgcttttaat gccacctacc agcaaatcag
      421 ggaaaatatg tctgagtttg cacgttgcca ttatgggtat atacaaatcc ctccagtgac
      481 aactttccgc gccgacggac cagaaactcc cgaagaagaa aagggttact ggtttcatgc
      541 ttatcaaccc gaagatcttt gtaccatcca taatccaatg ggagatttgc aggattttat
      601 tgcattggtt aaagatgcta aaaaatttgg tatcgatatc attcctgatt atacctttaa
      661 ctttatggga attgggggta gtggtaaaaa tgacctggat tatccctctg ctgatatacg
      721 agcgaagatc agtaaagata tagaaggtgg tatccctggc tattggcaag gtcaggtttt
      781 gattccattc attaaagatc cagtaacaaa agaacgtaaa caaatccatc cagaagatat
      841 acatctcact gcaaaagact tcgaagcaag taaagataac atctctaagg atgaatggga
      901 aaacctccat gcattaaaag aaaagcgttt aaatggaatg cctaaaacaa cacccaaaag
      961 tgaccaggtt attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg
     1021 aggtttacgt tatgatgcgg caaaacactc aaaacatgaa caaatagaaa gatcaataac
     1081 cccaccgctt aaaaattata atgagcggtt acacaatact aacttattta acccaaaata
     1141 tcataaaaaa gccgttatga attacatgga atatctggta acttgtcagt tggatgaaca
     1201 acaaatgtca tcactgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct
     1261 catgaaaacg ataaaagcct tttcatttgg tggagatctc caaacccttg catcaaaacc
     1321 gggttccaca atctcaagta tcccatcaga aagacggata ttgattaaca ttaaccacga
     1381 ttttcctaac aatggtaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca
     1441 attagcaatg gcatatatgg ccgctctgcc gttcagcagg cctttagttt actgggatgg
     1501 ccaagtatta aaatcaacga ctgaaattaa aaattatgat gggtccacgc gtgttggcgg
     1561 tgaggcgtgg cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc
     1621 attatatata gataaagcag gaatatggag cgcatttgag ggtatatttg caactaaaaa
     1681 cgttctggcc tttagtcgtg gggattctgt gaacattaat c
//
LOCUS       DAZFAH010000386         1713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFAH010000386 DAZFAH010000000
VERSION     DAZFAH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.389"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1311
                     /locus_tag="ACHJWB_004972"
     CDS             <1..1311
                     /locus_tag="ACHJWB_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4316420.1"
                     /translation="THRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWR
                     RERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE
                     QAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCG
                     LTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGTTEWCAEYDEWGNLLNEEN
                     PHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPV
                     QYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVF
                     KTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPP
                     GYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1308..>1713
                     /gene="ybfC"
                     /locus_tag="ACHJWB_004973"
     CDS             1308..>1713
                     /gene="ybfC"
                     /locus_tag="ACHJWB_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4316421.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSD"
BASE COUNT          493 a          401 c          456 g          363 t
ORIGIN      
        1 acccaccggt accattacga cagtcagcac cggctggtgc actacacgcg gacacaatat
       61 gcagagccgc tggtcgaaag tcgctatctt tacgacccgc tgggccgcag ggtggcaaaa
      121 cgggtatggc ggcgtgaacg ggacctgacg ggctggatgt cgctgtcacg gaaaccgcaa
      181 gtgacctggt acggctggga cggcgaccgc ctgaccacga tacagaacga cagaacccgc
      241 atccagacga tttatcagcc ggggagcttc acgccactca tcagggttga aaccgccacc
      301 ggtgagcagg cgaaaacgca gcgccgcagc ctggcggata cccttcagca gtccggcggc
      361 gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga ccggctggaa
      421 agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc atcgtgcggc
      481 ctgacggtgg agcagatgca aaaccagatg gacccggtgt acacgccggc gcgaaaaatc
      541 cacctgtacc actgcgacca tcgcggcctg ccgctggccc ttatcagcaa ggaagggaca
      601 acagaatggt gcgcagaata cgatgaatgg ggcaacctgc tgaatgaaga gaacccgcat
      661 cagctgcagc agcttatccg cctgccgggg cagcagtatg atgaggagtc cggcctgtat
      721 tacaaccgcc accgctatta tgacccgctg caggggaggt atatcactca ggatccgatt
      781 gggctgaagg ggggatggaa tttttatcag tatccgctga atccggttca gtatatagat
      841 tcaatgggac tggcatcaaa atatggacac ttaaataatg gcggatatgg agcgagaccc
      901 aacaaaccgc ctacgcccga tccaagtaaa ttgccggaca tagcgaaaca attaagactg
      961 ccatatccta ttgaccaggc cagtagtgcg cctaatgttt tcaaaacatt cttcagagca
     1021 ttaagccctt acgactacac actgtattgc aggaagtggg taaaaccaaa tctgacttgt
     1081 acgccacagg atgattccca gtatccaggg atggatacaa agacagcaag tgattacctg
     1141 ccacagacaa attggccaac aactcaatta ccaccaggat atacttgtgc agaaccctat
     1201 ttattcccag acattaataa acccgatggg ccagcaacag cagggataga tgatttgggt
     1261 gaaattttag ctaaaatgaa acagagaaca tcgagaggaa taagaaaatg aaaagagttt
     1321 tgttcttttt gctgatgata tttgttagtt ttggtgttat agctgattgc gaaatacaag
     1381 ctaaagatca tgattgtttt actattttcg ctaagggtac tattttttcc gcgtttcctg
     1441 tcttaaataa taaagccatg tggagatggt atcagaatga ggacatcggt gagtattatt
     1501 ggcaaacaga actgggtaca tgtaaaaata ataaattcac accaagcggg gcgagactct
     1561 taattcgcgt cgggtcacta cgccttaatg aaaatcatgc catcaaagga acgttgcagg
     1621 agttgataaa tacagcagaa aaaacggcat ttctcggcga tcggtttaga agttatataa
     1681 gagcgggtat ctatcaaaaa aagagcagtg atc
//
LOCUS       DAZFAH010000387         1697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFAH010000387 DAZFAH010000000
VERSION     DAZFAH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.390"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..641)
                     /locus_tag="ACHJWB_004974"
     CDS             complement(<1..641)
                     /locus_tag="ACHJWB_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4316422.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART"
     gene            910..1026
                     /locus_tag="ACHJWB_004975"
                     /pseudo
     CDS             910..1026
                     /locus_tag="ACHJWB_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1131..1207)
                     /locus_tag="ACHJWB_004976"
     tRNA            complement(1131..1207)
                     /locus_tag="ACHJWB_004976"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Arg,seq:tct)
     gene            complement(1221..1297)
                     /locus_tag="ACHJWB_004977"
     tRNA            complement(1221..1297)
                     /locus_tag="ACHJWB_004977"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Arg,seq:tcg)
     gene            complement(1305..1380)
                     /locus_tag="ACHJWB_004978"
     tRNA            complement(1305..1380)
                     /locus_tag="ACHJWB_004978"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Ile,seq:cat)
     gene            complement(1409..>1697)
                     /locus_tag="ACHJWB_004979"
     CDS             complement(1409..>1697)
                     /locus_tag="ACHJWB_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316423.1"
                     /translation="TDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMR
                     VAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          418 a          472 c          419 g          388 t
ORIGIN      
        1 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgtgc cgaatcctgg
       61 ctggcccccg tgtccgcact gaatgtcccc tcagcgccct gggtgaatgc agaaccacca
      121 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
      181 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      241 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcagccgg aatgatgtcg
      301 ttatacgtac aactctctcc acccggagtt accgtgctgc ggcgcgccag ctgtttaatg
      361 cgcggatccg gagcatcgta agaatccggt aacggaagcc cttcaccgta ggccatgccg
      421 ttggactgtc cggcaagcac aaccacgtag aaccagtccg gctcagatga agggccgacc
      481 tgtggatctc cttcaatagc caccgcctgc atcagtgtgt acggcgtaat ggcaaccggt
      541 ccgccgtatg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      601 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      661 aatattgtga gtaaaatgag gagcgggctg aagtccggaa gttacaggac aatggcagaa
      721 gagagacaac agcccgcaat acgaaaaagg ccgcgctatt gcgcagagtg attactgtcg
      781 gatattattc gccagctgaa atattacttc acgttttgtt gtttattcct tgccgcccgc
      841 gtctccctgc gcgggctttt tttgtccata agaaagcccc tccggagagg ggctggagag
      901 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      961 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
     1021 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
     1081 tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg
     1141 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     1201 acaacgcagg atacagataa tggaccgcct tcggggaccc gaactccgcg caaccagctt
     1261 cgaaagctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg cccttgctgg
     1321 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1381 gggtcaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg gatacatatc
     1441 tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac cagcttgaat
     1501 aaccgcattt ttctgctaca gcctgtccat atccatgccg ggaacataac atattcacag
     1561 caacacgcat ccgctcttcc agcaacaagc gactgaacat gtgcccttca tttttcagtt
     1621 ttatctttaa caaactctca ctcatatgca ggcgtaacgc aatcgcacca agcgtccagc
     1681 ttgctgatat atctgtc
//
LOCUS       DAZFAH010000388         1691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFAH010000388 DAZFAH010000000
VERSION     DAZFAH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.391"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            169..384
                     /gene="essD"
                     /locus_tag="ACHJWB_004980"
     CDS             169..384
                     /gene="essD"
                     /locus_tag="ACHJWB_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO4316424.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            389..700
                     /locus_tag="ACHJWB_004981"
     CDS             389..700
                     /locus_tag="ACHJWB_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4316425.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            697..1230
                     /locus_tag="ACHJWB_004982"
     CDS             697..1230
                     /locus_tag="ACHJWB_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316426.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1227..>1691
                     /locus_tag="ACHJWB_004983"
     CDS             1227..>1691
                     /locus_tag="ACHJWB_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO4316427.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIY"
BASE COUNT          472 a          351 c          434 g          434 t
ORIGIN      
        1 ttctcgtatt attattttat atatgacttt ctttcaaaat aattacccac atttttaatg
       61 tgtatgtttt tttagcgccg ttgagaacaa cgtgtgctgt caaaactacc ccgtagactc
      121 cgatcttttc aaacatattg caccatccgt gtacatcggg gtgaggatat gaaatcaatg
      181 gataagttaa caacaggtgt tgcctatggc acatcggcgg gtaatgctgg tttctgggca
      241 ttgcagttac tcgataaagt aactccgtca cagtgggctg caatcggtgt gctgggtagc
      301 ctggtttttg gcctgctgac gtatctgaca aatctttatt tcaagattaa agaagacagg
      361 cgtaaggctg cgagaggaga gtaatccaat gactcaagac tatgaactgg ttgtgaaagg
      421 agtccgtaat tttgagaata aagttacggt aactgtagcc ttacaggaca aagaacgctt
      481 tgacggtgaa atttttgacc tggatgtcgc catggaccgt gttgaaggag ctgcgctgga
      541 gttttatgag gcagcagcca gaaggagcgt ccggcaagtc ttcctggaag tagcagaaaa
      601 attgtcagaa aaagttgagt cttatctgca gcatcagtac tcctttaaga ttgaaaatcc
      661 tgccaataag cacgagcgtc ctcatcataa atatctatga acacaaaaat cagatacggc
      721 ctgtcggctg ccgttctggc gctgattggt gctggcgcat ctgctcctca gatacttgac
      781 cagtttctgg acgaaaaaga aggtaaccac acaatggcat accgcgatgg ttctggcata
      841 tggaccatct gtcggggtgc cacagtggtg gatggaaaaa ccgtttttcc caatatgaaa
      901 ctgtcgaagg aaaaatgcga ccaggtcaac gccattgagc gtgataaggc gctggcatgg
      961 gtggagcgca atattaaagt accactgacc gaaccacaaa aagcgggtat cgcgtcattt
     1021 tgtccctata acattggccc cggtaagtgt ttcccgtcga cgttttataa gcggctgaat
     1081 gctggtgatc gtaaaggtgc atgcgaagcg attcgctggt ggattaagga tggcggacgc
     1141 gattgccgca ttcgttcaaa taactgttac ggtcaggtta ttcgtcgtga ccaggagagc
     1201 gcattaacct gctgggggat agaacagtga atcagatatt catggtgatt tttctcgtgt
     1261 tgtcaggatt tatcgtcgga aatgtctgga gcgaccgagg atggcaaaaa aaatgggcgg
     1321 aacgtgatgc tgccgcatta tcacaagagg taaatgctca atttgctgct cgaataattg
     1381 aacaggggcg aactatagcc cgtgatgagg ctgttaaaga tgcgcaacag aaatctgctg
     1441 aaatttctgc cagggctgct tatctgtctg atagtgttaa ccagttgcgt gccgaagcaa
     1501 aaaaatatgc catacgcctt gacgcagcga agcataccgc agatcttgcc gctgccgtca
     1561 gaggcaaaac aaccaaaacc gccgaaggaa tgctcaccaa catgctcgga gatattgcag
     1621 cagaagctca gctttatgct gaaattgctg acgaacgcta catcgcagga gtgacttgtc
     1681 aacagatcta t
//
LOCUS       DAZFAH010000389         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFAH010000389 DAZFAH010000000
VERSION     DAZFAH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.392"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1690
                     /locus_tag="ACHJWB_004984"
     CDS             <1..>1690
                     /locus_tag="ACHJWB_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4316428.1"
                     /translation="NQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGTNRLGNFFV
                     GNGMADNVVLENGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADS
                     GATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGASPVTFH
                     KLTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYA
                     SMLTQAMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDALR"
BASE COUNT          375 a          448 c          527 g          340 t
ORIGIN      
        1 taaatcagaa cggtgaactg agggtacatg ccggcgggga agccactgca gtcacccaga
       61 acacgggcgg tgcactggtt accagtactg ctgcaactgt caccggcaca aaccgtctgg
      121 ggaatttctt cgtgggaaat ggcatggcag ataacgtcgt tctggaaaat ggcggtcgcc
      181 tggatgtact ggagggccat tcagcgcaga aaacacgggt ggatgacggc ggtaccctgg
      241 ccgtatctgc cggtggtaag gcaacagatg tcaccatgac atccggtggt gccctgattg
      301 cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt attgatggta
      361 tctccggtca ggccagcggc ctgctgctgg aaaatggcgg cagctttacg gttaatgccg
      421 ggggacaggc cggtaacacc actgtcggat atcgtggaac actgacgctg gctgccgggg
      481 gaagtctgag tggcagaaca cagctcagta aaggcgccag catggtactg aatggtgatg
      541 tggtcagtac cggcgatatt gttaacgcag gagagattcg ctttgataat cagacgacac
      601 cggatgccgc gctgagccgt gctgttgcaa aaggcgcctc cccggtaacg ttccataaac
      661 tgaccaccag taacctcacc ggtcaggggg gcaccatcaa tatgcgtgtt cgccttgatg
      721 gcagcaatgc ctctgaccag ctggtgatta atggtggcca ggcaaccggc aaaacctggc
      781 ttgcgtttac aaatgtcgga aacagtaacc tcggggtggc aacctccgga cagggtatcc
      841 gggttgtgga tgcacagaat ggtgccacca cagaagaagg tgcgtttgcc ctgagtcgcc
      901 cgcttcaggc cggcgccttt aactacaccc tgaaccgtga cagcgatgaa gactggtacc
      961 tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg ctgacacagg
     1021 caatggatta tgaccggatt ctggcaggct cccgcagcca tcagaccggt gtaagcggtg
     1081 aaaataacag cgtccgtctc agcattcagg gcggtcatct cgggcacgat aacaacggtg
     1141 gtattgcccg tggggccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg
     1201 gtgacctgct gagaacagag gttgccggta tgtctctgac gacaggggtg tatggtgctg
     1261 caggccattc ttccgttgat gttaaggatg atgacggctc ccgcgccggc acggtccggg
     1321 atgatgccgg cagtctgggc ggatacctga atctgacaca tacatcctcc ggcctgtggg
     1381 ctgacattgt ggcccaggga acccgccaca gcatgaaagc gtcatcgggc aataacgact
     1441 tccgcgcccg gggctggggc tggctgggtt cactggaaac cggtctgccc ttcagtatca
     1501 ctgacaacct gatgctggag ccacaactgc agtatacctg gcagggactt tccctggatg
     1561 acggccagga taacgccggt tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg
     1621 ccggatttcg tctgggcagc cacaacgata tgacctttgg cgaaggcacc tcatcccgtg
     1681 acgccctgcg
//
LOCUS       DAZFAH010000390         1682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFAH010000390 DAZFAH010000000
VERSION     DAZFAH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.393"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..409
                     /locus_tag="ACHJWB_004985"
     CDS             <1..409
                     /locus_tag="ACHJWB_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4316429.1"
                     /translation="ALTNNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGR
                     VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLT
                     LGLNALTLTDVTKNAAYGVEIESLVLEINAPAA"
     gene            702..908
                     /locus_tag="ACHJWB_004986"
     CDS             702..908
                     /locus_tag="ACHJWB_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316430.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKTARGD"
     gene            913..1248
                     /locus_tag="ACHJWB_004987"
                     /pseudo
     CDS             913..1248
                     /locus_tag="ACHJWB_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
BASE COUNT          463 a          347 c          465 g          407 t
ORIGIN      
        1 cgcactgacc aacaatcagt tcgggctggg gatttatctg tatacggatg ctcccgttcc
       61 tgatggtgtg gcgatgacgg gtaccggtaa tccgttcctg atgtcgtact tcactcagac
      121 cactgacggc agagtgaatc tgatgcatca caggaaagcc ggaaacacga agctggggga
      181 gttcggcgat tacggtaacg actggcagac gctggagctg gtgttcaccg ccggcagtgc
      241 cacggttact ccgaaactga atggagtggc tggcccggca ttccaggtta taaaagacag
      301 tctgacactg ggactgaatg cgctgacgct gacggatgtt acaaaaaatg cagcgtatgg
      361 cgttgagata gaaagtctgg tgctggagat aaatgcaccg gcagcataat aaaaaaagcc
      421 agcgcccact ctgaaggacg ctggctaaaa caggtagatg tacttcacat gatacttata
      481 tttggcagta cattttctga cagacagtga cggatgttgt caagatattg tgtcatttat
      541 aacctgaatc aggggttggt cggaatgtta tctggcattt ttagcagagc ctgaatgcca
      601 taatcacggc tcccggagtt ggccgtcagt gggtgacact ggcggctttt tgttttcctt
      661 tactttcatt ttctgtcggc ggtgacggag acatacatca gatggaaaaa atcacaacgg
      721 gtgtgtcata caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca
      781 aagtctctcc gtcccagtgg gtggcaatag gtgtactggg gagtctgctg tttggcctgc
      841 tgacgtatct gactaacctg tatttcaaaa tcagggagga ccgccgtaag acggcgcggg
      901 gagactaaag cgatgaagaa aaaatacgaa ctggttgtta aagagataaa taattacccg
      961 gataagattg ctgttactgt ggcacttgaa attggcgggc atccgtcgtt gttgttgcca
     1021 catgtggcga ttagtcttga ccgtactgaa ggtgccacgc tggagttcta cgaagctgag
     1081 gcgaaaaaac aggcgaagca gtttttcatg gatattgttg ccgggttatg cgaaggggat
     1141 gaaccgtcac cggaaaagcg ccccgtaatt ttagatgcgc aggatgtatt gataacctac
     1201 aaaggaaagc taccgggaag aattacttgt tctctgaaga tgccgccgtc aacactgcgg
     1261 tcagaaaaag atgatgttga atcgcgtatt gaaaaactgg agtgctatat cgctgaattg
     1321 aaaaaaagca ccccaacaaa aaatgaggtg cttgcagcag acgaaatgaa agaagctatt
     1381 cctgatcgcg cggcgaatct aagctgcgct tcatggttga aagagcatct tcagcagcct
     1441 gaaaaaaaac gccgcgatga gcagtttgct gcgttttacg attattgccg gaaagtgatg
     1501 agcagaaatc tcgcagagtg tttcagtatt cataatgata atttcagtga cctggaatgg
     1561 gagtgtaacc ggccatcctt tgttgtatcc ggtgatgctg ggaaaataac catctcagaa
     1621 aatggaaaag taacaccgcc atcgcaccag catagtgagg agctcattga atttgccatt
     1681 ga
//
LOCUS       DAZFAH010000391         1678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFAH010000391 DAZFAH010000000
VERSION     DAZFAH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.394"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..220
                     /locus_tag="ACHJWB_004988"
     CDS             <1..220
                     /locus_tag="ACHJWB_004988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316431.1"
                     /translation="TFFVNIDKFADREEISVNHRPVIHFLSEKNEMLIFSMCDEIATN
                     TRDYLYLWIFPEPEESVHDADVREESSE"
     gene            509..1206
                     /locus_tag="ACHJWB_004989"
     CDS             join(509..758,758..1206)
                     /locus_tag="ACHJWB_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFO4316432.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGCLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRRHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            1223..1486
                     /locus_tag="ACHJWB_004990"
                     /pseudo
     CDS             1223..1486
                     /locus_tag="ACHJWB_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            1498..>1678
                     /locus_tag="ACHJWB_004991"
     CDS             1498..>1678
                     /locus_tag="ACHJWB_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4316433.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQL"
BASE COUNT          446 a          405 c          412 g          415 t
ORIGIN      
        1 tacttttttt gtgaatatag ataaatttgc tgaccgtgag gaaatatcag tgaatcaccg
       61 accagtaatc cacttccttt cagagaaaaa cgagatgcta attttttcga tgtgtgatga
      121 gatcgccaca aacactcgcg actatctgta tctctggata tttcctgaac cagaagagtc
      181 agtgcatgat gctgatgtcc gggaagaaag ctcagagtaa ggatgagtcc tgtccctgga
      241 cttcatctga ctgatttcat ataaggaaac taccaccatt gattctctgg cgtttcgaac
      301 tatcacggat aatgaaaaaa acagcaaagc taccgcacat tatttgcaaa gacgcaaata
      361 atgtaaaatt tgtcatctgt tctaaatcct aacaaccagc atggtaatac tgtcaatcaa
      421 atatagataa aatgcataag tgaatcgccg tagggtgatg ctgccaactt actgatttag
      481 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
      541 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct
      601 ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg
      661 tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg
      721 cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg
      781 gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag
      841 tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacaggctc
      901 cggaagacgg ttgttgcgca cgtattcggt gaacgcacga tggcgacgct ggggtgtctt
      961 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
     1021 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
     1081 cggcataacc tgaatctgag gcggcacctg gcacggctgg gacggaagtc gctgtcgttc
     1141 tcaaaatcgg tggaactgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
     1201 caataagttg gagtcattac ccacggaaac agacaacgcc tggttgtact cgctgtcaca
     1261 ccagaccagt gacttcggcg aatcagaatg gattcatttt accggtagcg gatatctgtt
     1321 acgtaccgat gcgtggtcat acccggttct gcaacttaaa cgtctgggcc tgtcaaaaac
     1381 attccgtcgt ctggttgtca cactcatccg gcgttatggc gtcagtctca ttcacctgga
     1441 tgccagcgct gaatgcctgc cgggtttacc cactttcaac tggtaaccag gaacaacatg
     1501 aaattattaa ccacggaaac cgcactggat attctgattg cgtggctgca ggacaatatc
     1561 gactgcgaat cgggaattat cttcgacaac gatgaggata aaacggattc agcagcactg
     1621 ttgccctgta tcgaacaggc cagagaggat atccgtaccc tgcgccaact gcagcttc
//
LOCUS       DAZFAH010000392         1673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFAH010000392 DAZFAH010000000
VERSION     DAZFAH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.395"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..824
                     /locus_tag="ACHJWB_004992"
     CDS             <1..824
                     /locus_tag="ACHJWB_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4316434.1"
                     /translation="VAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLW
                     MAHTGTFGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQ
                     TLALPSRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESD
                     PSYLDVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISL
                     GEEWVELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            833..1198
                     /locus_tag="ACHJWB_004993"
     CDS             833..1198
                     /locus_tag="ACHJWB_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4316435.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            1213..>1673
                     /locus_tag="ACHJWB_004994"
     CDS             1213..>1673
                     /locus_tag="ACHJWB_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4316436.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAV"
BASE COUNT          378 a          425 c          517 g          353 t
ORIGIN      
        1 gcgtggcggg tatgggcgat cgccagtaca actatgtcgt catgccctat aaggatgatg
       61 caaacctgaa aatcatcagt gatgagcttc tgaaacgctg gggaccggcc aaaatgtctg
      121 acggtgtgct gtggatggcg cataccggca cgtttggtga ggtgcaggca ttcggggcaa
      181 aacgcaatga ttttctggcg acctgcacat tcattcccaa agcccctgaa ccggattacc
      241 tctgggcagc ggcggtctgc gccacctgtg cgccgtctct gggggcagat cctgcgcgtc
      301 cgcttcagac gctggcgctt ccgtcacgca tggccccggc cccggccgat cgtctgacgc
      361 gtgaagagcg taacagtctg ctgtggggag ggattgccac ggtcagtgtg gctgccggtg
      421 atgtggttca gatcgaacgt caggtgacca tgtatcgcca gaacgtgtac ggcgaaagtg
      481 atcccagcta tctggatgtg gaaaccatct acaccctctc ctggctgcgt tattccctgc
      541 gtacttttat cacacaacgt tttccgcgcc ataaactggc tgatgacggg acgcccgttc
      601 gtgccggtca gaatatcgtg acgccggaga tcatgaagct tcagttaatt tcgctgggtg
      661 aagagtgggt ggaactcggg ctggtcgaaa atctggacac ctttaaaaag aatctgcttg
      721 tggagcgtaa tgcgtcagat cgaaaccgtc ttgacgtgtt atgcacgccg gatctggtta
      781 accagttccg cttcctggca gcacagatcc gtttcatttt gtgaggtaag gcatgagcgg
      841 aaaacagtat cagggcacgg ccactatccg tgtgaacggt caggaatacg agacgcttga
      901 gggggctaca ttttctccgt caggttttga acgtgaagtg gtgaaaggcg cgaaagtcta
      961 tggttaccgc cagaaaccac gtgaagcgac actggactgc aaatttccgg caggcggaga
     1021 aggttcacca gccgccgatg aaatcaacaa ctggactgct gtcactatcg agtttgtggc
     1081 ggataccggc gaagtccaca tgatgacgaa ggcctggagc agtgaaccgg cctcgcttga
     1141 cggtggcggg gagatctccg tgaagttcgc cagcgccacc agtacccgtg ttcagtgatc
     1201 aggaaaaata aaatgaccac acgtaagaaa aaaacggcag tttccgaggc agccgtgatg
     1261 gaggcaatcc gggaagcact tgagggggct gatccacgca ctgccgggct gacagagcaa
     1321 ctggcaaagg gatatgtgga tctgcttgat ggtctgccgt ttggtgagac ccgtgaatat
     1381 cgtgtcacgt tccgggaact gacagcgaaa gacagtattg acgcagaagc agaagccgag
     1441 cgcgtggtgg agacaaacaa tggcccgatg ctgatagcgt ctccgtcgtt acgcggtgtt
     1501 gcactgctgc gccgtcagat tgccgcagtg ggagacattg aaggtccgtt gtcaccccgt
     1561 cagattggac agttaagcga gcgcgatctc tcccgcctga tggcagcggt cagcctgctg
     1621 gataccgcgc ttgccggaaa gctggcagct gaccgggggc gatcaggcgc agt
//
LOCUS       DAZFAH010000393         1667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFAH010000393 DAZFAH010000000
VERSION     DAZFAH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.396"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..1247
                     /locus_tag="ACHJWB_004995"
     CDS             15..1247
                     /locus_tag="ACHJWB_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4316437.1"
                     /translation="MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSA
                     EYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLRKRYEMTNLIP
                     PINSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1250..>1667
                     /locus_tag="ACHJWB_004996"
     CDS             1250..>1667
                     /locus_tag="ACHJWB_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO4316438.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFL"
BASE COUNT          515 a          315 c          343 g          494 t
ORIGIN      
        1 atgaggtaaa tataatgaag ctttcaaata tcgtcacagt tataattctt gcaatttcca
       61 gtacgctaac accccaggcg atggcagaaa aattaattcc tgaaactgat gctgagttgt
      121 tagtttggtc tgatgccaca agcgcggaat atatgaaata tgcagcaaag gaattcaata
      181 aagattttgg ctacaaggta aagtttacat ttcgcaatat agcgccaatg gatgcagcat
      241 caagaattat gcaggatggg ggtacgactc gtgtagctga tgtagctgaa attgaacatg
      301 ataccctggg gcggttagtc gttgctggcg gggttatgga aaacatggtc tcagctgagc
      361 gcattaaaaa aacatttatt ccaggcgcag tatcggcagc tacatataat aacatcagct
      421 atggttttcc tgtaagtttc gcaacgctgg cgcttttcta taataaggat ttgttaaaca
      481 ccgcaccaaa aacattcgaa gaaatcaata ctttcagtga aaagtttaat aattcatccg
      541 agcataaata tgctctgcta tgggatgtac aaaattatta tgtttcacgt atgtttatta
      601 ccttgtatgg tgccaacgaa ttcggtaaaa ccggtaacga tcctaaagct ctaggcatcg
      661 cttcatctga agcgaagaaa gggctagaga cgatgaaacg cttaaagaaa gcgaatccct
      721 ctaatcctct tgatatgggt aatccacaag ttctaagagg tctgtttaat gaaggtaaag
      781 ttgctgctgt aatcgacgga ccttggtcca tacaaggtta cattgacagc ggaatcaatt
      841 ttggcgtgac acgcatccca acattagatg gtcatcagcc tcgcacattt tcaacagtac
      901 ggctggccgt tgtaagctca tttaccgaat atcctcatgc cgctgaatta ttcgctgact
      961 acctgacaac ggataagatg ttgaggaagc gttatgaaat gacgaatctc atcccaccaa
     1021 tcaattcact tatgaacaaa attagccaga caggtagtga agctataaaa gcaattattg
     1081 ctcaagctaa ttattctgac gcaatgccat caataccgga aatgtcttat ctatggtctc
     1141 caatgactaa tgctattttg gctacctggg ttgagaataa aacaccggat gaagttttaa
     1201 atcatgcaca aacaattatt gaagaacaac tttcgcttca ggagtaattg tgaatacaca
     1261 acaactgaaa atgaagagtg cacctgtgct acctatttcc tgtttgatta tgggcgggac
     1321 tcagttatca cgtcattatt atgtgaaagg cgggatattc tttgcaatac aagtatgctt
     1381 tttgctttat ctttccgata ttgtgcatac attaataggt ttgttcacat taggtgatgt
     1441 cgcgcaaatt cgaaaagggt tgactgttat tcagggtgac aattctatat ttatgcttgt
     1501 agagggcgtg atagcgacca ttattgttgg cttattcgcc actatttaca tccttaatat
     1561 taaagatgcg cggaactcat cttattgcca tctgactttt aaacaacagt tatataagct
     1621 ctatgaggat aaatttgcat ttatagtcct aacgcccgca tttttag
//
LOCUS       DAZFAH010000394         1664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFAH010000394 DAZFAH010000000
VERSION     DAZFAH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.397"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1663
                     /locus_tag="ACHJWB_004997"
     rRNA            <1..1663
                     /locus_tag="ACHJWB_004997"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          421 a          368 c          527 g          348 t
ORIGIN      
        1 gctggaggta tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc
       61 gctcgccgga agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc
      121 taaggcgagg ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt
      181 tactgcgaag gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag
      241 cgtgtaggct ggttttccag gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc
      301 actacggtgc tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa
      361 catcaaatcg taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag
      421 agaactcggg tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga
      481 tatgtaggtg aagcgacttg ctcgtggagc tgaaatcagt cgaagatacc agctggctgc
      541 aactgtttat taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg
      601 cctgcccggt gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag
      661 ccccggtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
      721 aagttccgac ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt
      781 gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa
      841 cctttactat agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt
      901 tgaagtgtgg acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga
      961 tgttctaacg tggacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg
     1021 ggcggtctcc tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca
     1081 tcaggaggtt agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg
     1141 tgcgaaagca ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat
     1201 aaaaggtact ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt
     1261 ttggcacctc gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc
     1321 tgttcgccat ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc
     1381 ctatctgccg tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag
     1441 tggacgcatc actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg
     1501 gaagagataa gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg
     1561 actccttgag agtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa
     1621 gcgcagcgat gcgttgagct aaccggtact aatgaaccgt gagg
//
LOCUS       DAZFAH010000395         1641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFAH010000395 DAZFAH010000000
VERSION     DAZFAH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.398"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(155..664)
                     /locus_tag="ACHJWB_004998"
     CDS             complement(155..664)
                     /locus_tag="ACHJWB_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HFO4316439.1"
                     /translation="MSSSLSGITFTTFLTSNHASWNKLTRTLHISNDDILDELQLKAR
                     MQQRHHRVFAGIDDYSIINFRGKDYAVRFIKDNDNYVYKVNKITQESGCFSTIFSFLY
                     GDVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     LNSLSGVTV"
     gene            complement(695..1585)
                     /gene="escQ"
                     /locus_tag="ACHJWB_004999"
     CDS             complement(695..1585)
                     /gene="escQ"
                     /locus_tag="ACHJWB_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO4316440.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSEGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDVYPVIENNNGENRLNVIILETTVQSLQHLINDNWQFVPHSNALFFDGYI
                     VPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINNPLATVKLSDNNLSVTDVLAAD
                     PLCTISSETVMNRIYCVSGTVHIDIHMLRNVQKDDIIDSTGYHLFGGCQLVRNNTTIA
                     YGSIIKINEDFYFTISVVCD"
     gene            complement(1575..>1641)
                     /locus_tag="ACHJWB_005000"
     CDS             complement(1575..>1641)
                     /locus_tag="ACHJWB_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO4316441.1"
                     /translation="FDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          530 a          354 c          269 g          488 t
ORIGIN      
        1 gcatccattc agttcatatt tcacaaaaac tacagtgctc tatccttgaa atactaatca
       61 tttcgcctgc atattctttt caaaaaataa tgaaatatcg cccccagaac agaacacatt
      121 ctggggagaa aaagaaaaca gcataaaata atacttaaac tgtcacacct gataaagagt
      181 ttaatgtagt attctctgga gggttcacac cttctgtaga ctgtacgcga cgtaacaaag
      241 aagagagccc tctttcagtt aaaaagcctt ccagtgcagt gcgaggaaac caggttgaag
      301 aaagtggcgt aatgtgtctt tcatttaatt tactttccag ggccttcgta acgtctccat
      361 acaaaaagct aaatatcgtt gagaaacatc cactttcctg agttatctta tttactttgt
      421 atacataatt atcattatct ttaatgaagc ggacagcata atcttttcca cgaaaattga
      481 taattgaata gtcatcaatg cctgcaaaaa cccggtggtg acgctgctgc atgcgtgctt
      541 taagttgcag ttcatctagt atatcgtcat tcgatatatg aagagttcgg gtcaacttat
      601 tccatgacgc atgattgctt gtcaaaaaag tagtgaatgt tatacctgat aatgatgacg
      661 acatcataca cctccctata taacgcattc ctgattaatc acatactacg ctaatagtaa
      721 aataaaaatc ttcgttgatt tttataatac tcccgtatgc gattgtggta ttattccgta
      781 ctaactggca cccaccaaaa agatgatatc ccgttgaatc gataatgtca tctttttgga
      841 cattacgcaa catatgtata tcaatatgca ctgtgccact cacacaataa atgcgattca
      901 tcacagtctc gctgctgata gtacataacg gatctgcagc cagaacatcc gtgacagata
      961 aattattatc gctcagcttt acggttgcca aagggttatt aataacaatc cagcaccttc
     1021 tctcttgact atcatcaaca tgatagagtc tcagactatc accaactctc aactctgtaa
     1081 ctggataatc agtccaccca ggtacgatat agccatcaaa aaaaagagca ttagaatgtg
     1141 gaacaaattg ccaattatca ttaatcagat gttgtaaact ttgtaccgtt gtttcgagta
     1201 taattacatt aagtctgttt tctccattat tattttctat taccggataa acatctttat
     1261 ttagcatcgt aaattgaaca tcatcaactg acacatcacc ttccggtaag gcagtctgca
     1321 ttacccataa gatcatgagt tttatcatat cttccggtat cagccggctt tcactatacg
     1381 ggaataactc ttggcaccac tgaatgaact gttcttctga tatccagaaa cgcccctcat
     1441 agcccgagta tgtcaacgtg aaacgaatac catgaccgct gacctcacga aaatatgcat
     1501 ggcaagcatg accttcggac gtaatataga ggcgaccggt tccgataact ggcaacaacg
     1561 gcaaataaaa atcgttaatt ttcatattca attgtgaact caatggcttc atcaggaaag
     1621 atttctttta acttatcgaa a
//
LOCUS       DAZFAH010000396         1641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFAH010000396 DAZFAH010000000
VERSION     DAZFAH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.399"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /gene="ymgF"
                     /locus_tag="ACHJWB_005001"
     CDS             <1..183
                     /gene="ymgF"
                     /locus_tag="ACHJWB_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO4316442.1"
                     /translation="YIIFSIAFMLITLLVILIAKPSTGLGEVLVTINLLNALVWLAIN
                     LVNRLRERLVNHRDQQ"
     gene            583..792
                     /locus_tag="ACHJWB_005002"
     CDS             583..792
                     /locus_tag="ACHJWB_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316443.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            909..>1641
                     /locus_tag="ACHJWB_005003"
     CDS             909..>1641
                     /locus_tag="ACHJWB_005003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4316444.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSA"
BASE COUNT          474 a          314 c          378 g          475 t
ORIGIN      
        1 tatatcatct tttccattgc atttatgctg atcaccctcc tggtcatcct tattgcaaaa
       61 cccagtaccg ggctgggaga agtgcttgtg acgataaatt tgcttaatgc ccttgtttgg
      121 ctggcgatca atctggttaa tcgattaaga gaaagactcg tcaaccacag ggatcagcaa
      181 taatctttca gtttctcact gtcagtatgc ggctgaatgg gttgctggca gtgaacgcct
      241 ggatcattga aggaaaggca ttattgcgca aatagttgtc aaccctggtg ttatcacggt
      301 tgtttttata tatcaccgaa ataatcctca tcgcaactat taataatttt gatgtcgaag
      361 agttatttgt taaacaaaat cgtcacccta aagtgatcaa tgtcatgaaa ataaggtgaa
      421 aaatgataat gccgacttat ttatcattta tatattgtcg ctgtttatct atttaaattt
      481 tttgtgcttt tgtttttgtc tttaccctcc gttttggtaa tctgaacgca taggttattt
      541 gaccaggaat gtttgttaat ccaatgaatg gatggttaat ctatgaaact gaaaaaactc
      601 tccgggttta gtttgggact tattgctctg gcggtgggta atgcatatgc tgaacagttt
      661 attcagcacg atgaatataa acatgctgtg gcaataaaag atggaaaagt aaaagatatt
      721 gataatgcgc tagttataac aacgggtaat ggaagttacg ggtatccgtt gcaggagagg
      781 gtagcaagtt aaccattaac aatggcgtca ttgtaacaac gggcggttta tatccttctg
      841 acaatagtcc tctaagcaca gcattaacag ctagcgcaat cgtaagtgaa tttggcggtg
      901 ctgttatttt gcagggtaaa aacacgattg tcataacggg ggattactca attggtttgc
      961 taagtcaaac gagtggaaac ctgaatactg acaccataat aagagtcaac tctgacggtt
     1021 cggttacccc ctctttttct gacggagatg atacatttat tgttactgcg ggtaatcacg
     1081 cagtcggcgt tcttgcgtgt gcatctcccg gaagcgcgcg tgcgtgtgta tcttctcttg
     1141 atgaagaaag tactaccgat acaggaagta atgaaaataa tgccatagca aaactggata
     1201 tggcaaaagg ggagatcaca acccacggaa cagaaagtta tgccgcgtac gctaatggca
     1261 ctgtcgtcaa ggctggtgac acgcttgatt ataccaatgc cagcgttaca ttaaccgatg
     1321 ttgatatcac cacgcatggg gacaatgctc atgccatcgc tgcccgccag gggacagttt
     1381 catttaacca gggagaaatt tatacaacgg gtcctgacgc cgcgattgct aaaatttata
     1441 atggcggcac ggtgacgctg aaaaatacat ctgcagtcgc gcatcagggg tctggaattg
     1501 tgctggagtc ttccataaat ggtcaggaag caacggtaga tattttatct ggtagctcac
     1561 tacggtcagc aaatgaaatc ctctaccata aaaatgagac gagtaacgtg accattacgg
     1621 atagtgaggt atcatcggct g
//
LOCUS       DAZFAH010000397         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFAH010000397 DAZFAH010000000
VERSION     DAZFAH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.400"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..438)
                     /locus_tag="ACHJWB_005004"
     CDS             complement(13..438)
                     /locus_tag="ACHJWB_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4316445.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDPPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..703)
                     /locus_tag="ACHJWB_005005"
     CDS             complement(422..703)
                     /locus_tag="ACHJWB_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO4316446.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            803..1222
                     /locus_tag="ACHJWB_005006"
     CDS             803..1222
                     /locus_tag="ACHJWB_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316447.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1489..>1634
                     /locus_tag="ACHJWB_005007"
     CDS             1489..>1634
                     /locus_tag="ACHJWB_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4316448.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGPHRW
                     LARNS"
BASE COUNT          484 a          392 c          348 g          410 t
ORIGIN      
        1 ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa ctgcatcagg
      241 ggggtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tacagttcag gaaacgtcat acccaactca aaataagcct gggttattcc
      361 agctgctgga actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcaagctcc tagtaagtgg ttgtgttaac gttttggtat
      481 cttccagctc gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt
      541 caccattact ggctcgctca ataaggacac ataacgatgc ccctaacatt tgacctttac
      601 tcaatgcctt tcttagataa ccgatgctag taccacactc acatgcaaac atacgctgtt
      661 catctgacga aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa
      721 cacagcaaga atacctacag gtaaacaaaa gtcaataccc ttgggttgtt taccttgcgg
      781 taatcgcatc tattatttac ctatggacaa atatgaattt agacgacaac aactcatcaa
      841 aattcgtgat gagaaatgcg atggtaaagc ggttaacgtg gccagaaaga tcggtcgcga
      901 gccttcttat gtatcaagaa tgttgtaccc agaggggaaa aaaggaaaaa aacggatcgc
      961 tgatgatatg gtggagatta tagaagagtc ctttgggtta ccccggggat ggatggatgg
     1021 tatcgtttca tcatcaacga acacagcctc caattatgaa acaagggttc taacgccacg
     1081 acaacgtatt tttttagatc tcttagacga actgccagaa agtgaagcgg ataacttatt
     1141 aaaaactctt gaagagaaaa aacagtatta caatatgatc tacgaagaaa tccgtaaaaa
     1201 gaaagcacaa aacgcatcat aactcaccaa acaaccagtc accagttaag acacatcaaa
     1261 aaaattaccc atgggtattt actttttaaa tacccatggg tatccttcct ttcataccaa
     1321 cccaccccgt cccacagaac gcagggcaat acttcgagtt accaggcagt ggtcaggggg
     1381 taagtagcca gcccgaggcg taagaacatg acggcagggt tcaactttaa taactatgca
     1441 gcaggttttt gttccgctac cccggcgtta aggggaaatg aggtcagcat ggatactatc
     1501 gatcttggca acagcgaatc tctggtatgc ggcgtgtttc ccaaccagga cggtacgttt
     1561 accgcgatga cgtataccaa aagtaaaacg tttaaaaccg aagctggccc gcatcgctgg
     1621 ttagcaagaa actc
//
LOCUS       DAZFAH010000398         1630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFAH010000398 DAZFAH010000000
VERSION     DAZFAH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.401"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1179
                     /locus_tag="ACHJWB_005008"
     CDS             <1..1179
                     /locus_tag="ACHJWB_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4316449.1"
                     /translation="QRAGLFPAEATWGGLLPSLVREYRYDSAGNVSGVTSREDYGRET
                     RREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDSYRPETERFRWDSRDQLTGYCSAQGELWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQF
                     SRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TGYPDPRGELDPEAAPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLLGGE
                     QTYRYVPNPLGWIDPYGLLCRRGQLKALGIPDSPGVYHIILDNKVYTGSGKSILNRLA
                     "
     gene            1213..1476
                     /locus_tag="ACHJWB_005009"
     CDS             1213..1476
                     /locus_tag="ACHJWB_005009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316450.1"
                     /translation="MFDGNNKLHENVKIFIQRVDLGNAQTPREIVRSLRHYEQKAMDY
                     FENIPKINGSQNGGRAAAVSKVEEFAKVAKEKLTSAGEWVILK"
BASE COUNT          424 a          371 c          501 g          334 t
ORIGIN      
        1 cagcgcgccg gacttttccc ggcagaagcc acatggggcg ggctgctccc ctcactggta
       61 cgggagtacc gttacgacag cgcgggtaat gtgtcaggcg tcaccagccg ggaagattac
      121 ggacgggaaa cacgccggga gtaccggctg gaccggaacg gtcaggtcac ggcggtgaca
      181 gcctcaggca ccgggctggg ctatggcgaa ggcgatgagt cctatggcta tgacagttgt
      241 ggctacctga aggcgcagtc tgcgggcagg caccggataa gtgaagagac tgaccagtat
      301 gccggaggcc accggctgaa acaggccgga aacacgcagt atgactatga cgccgcaggc
      361 cggatggtca gccgcaccag acaccgtgac agctaccgac cggaaacaga gcggttccgg
      421 tgggacagcc gggaccagct gaccgggtat tgcagcgcac agggtgagct gtgggaatac
      481 cgccacgacg ccagcggcag acgaacagag aaacgctgcg accggaagaa aatccgtttt
      541 acgtacctgt gggacggcga cagtattgcg gaaatccggg aataccgcga tgataaactg
      601 tacagcgtaa ggcacctggt gtttaacggc tttgagctga taagccagca gttcagccgg
      661 gtacggcagg cgcatccgtc ggtggccccg cagtgggtga cgcggacgaa tcatgcggtg
      721 agcgacctga cgggccgtcc gctgatgctc tttaacagtg aaggtaaaac cgtctggcga
      781 ccgggacaga ccagcctgtg ggggctggca ctcagcctgc ccgcagacac cggctacccg
      841 gacccgcgcg gggaactgga cccggaagcc gcccccggcc tgctgtatgc aggacagtgg
      901 caggatgcgg aatccgggct gtgctataac cggttccggt attacgagcc ggaaaccggg
      961 atgtacctgg tgagtgaccc gctggggttg ctggggggtg agcagactta ccggtatgtg
     1021 ccgaatccac tggggtggat tgatccatac ggtctgttgt gcagaagagg acaacttaaa
     1081 gcgttaggaa ttccggatag tccaggtgtt taccatatta tattggataa taaagtgtac
     1141 actggttcag gaaaaagtat tctgaatcga ttagcttgag gattgggagg gcacaaaaag
     1201 gctacagaat tattgtttga tggtaataat aaactgcatg aaaatgtgaa aatatttatc
     1261 caaagagttg acttgggaaa tgctcaaact cctagagaga ttgttcgctc tcttcgacat
     1321 tatgaacaaa aggcaatgga ttatttcgaa aacataccga aaattaatgg ttctcaaaat
     1381 ggggggaggg ctgcggcggt ttctaaggtt gaagagtttg ctaaggtcgc aaaagaaaaa
     1441 ttaactagcg caggtgagtg ggttatttta aaataaagga gcatttgaat gaactctttt
     1501 actctacgta gttcagaacg ttgggttctg catggagatg tatctcctga tgtattaaat
     1561 aaattcgcat atctcggaca aaaattatca tgggaacata ctgacatctc tcagaaggaa
     1621 tttattgatt
//
LOCUS       DAZFAH010000399         1619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFAH010000399 DAZFAH010000000
VERSION     DAZFAH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.402"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..546
                     /locus_tag="ACHJWB_005010"
     CDS             <1..546
                     /locus_tag="ACHJWB_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO4316451.1"
                     /translation="NLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDV
                     YTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQF
                     GLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRR
                     HSRLGNISPAAFREKYHQMAA"
     gene            497..1198
                     /gene="traT"
                     /locus_tag="ACHJWB_005011"
     CDS             497..1198
                     /gene="traT"
                     /locus_tag="ACHJWB_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4316452.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(1246..1497)
                     /locus_tag="ACHJWB_005012"
     CDS             complement(1246..1497)
                     /locus_tag="ACHJWB_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4316453.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1521..>1619)
                     /locus_tag="ACHJWB_005013"
     CDS             complement(1521..>1619)
                     /locus_tag="ACHJWB_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4316454.1"
                     /translation="SGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          460 a          392 c          380 g          387 t
ORIGIN      
        1 aatctgccag ttgcgccaaa tctgctgaac cagacgttcg ctcctacagc accaaatcag
       61 gtctgggtgg cggacctgac gtatgttgcc acacaggagg gatggttgta cctcgctggc
      121 atcaaagatg tttatacgtg cgaaattgtc ggctacgcca tgggagagcg catgacaaaa
      181 gagctgacag gtaaagccct gtttatggcg ctcaggagcc agcgcccacc tgccgggcta
      241 atccaccact ctgatcgagg ttcacagtac tgcgcatacg attaccgggt catacaggag
      301 cagtttggtc tgaaaacatc aatgtcgcgt aaaggtaact gttacgacaa cgctccgatg
      361 gaaagcttct ggggaacgct gaaaaatgag agcctgagcc actatcgttt taagagccgg
      421 gatgaggcca tctcagtaat acgggaatac attgagattt tctacaatcg tcagcgtcgt
      481 cactctcgtc tggggaatat ctccccggca gccttcaggg aaaaatatca tcagatggct
      541 gcttaaaaaa agaacaaatg gtagtgtccg ctattgtcag tacacctcaa ccatctggct
      601 tgaacctgcg agcgaacgca ccgttttcct gcagatcaaa aacacgtcgg ataaagacat
      661 gagtggacta cagggaaaaa ttgccgatgc agtcaaagcc agaggttatc agatcatgac
      721 ttccccggat aaagcttact actggattca ggctaacgtt ctcaaagcag acaaaatgga
      781 tctgcgtgaa tctcagggat ggctgagtcg cggttatgaa ggtgcagcag tcggagctgc
      841 tcttggaggt ggaatcacag cgtacaatac cgggtcggca ggtaccactc tcggtgttgg
      901 actggctacc ggtctgattg gcatggccgc ggatgcaatg gtggaagata taaattacac
      961 aatgattaca gatgttcaga ttgccgagcg aaccagaacc agcgtcagaa cggataatgt
     1021 tgcagctttg cgtcagggaa catcaggctc caaaattcag acaagcacag aaaccggcaa
     1081 ccagcataaa taccagaccc gggttgtatc cagtgccaac caggttaatc tgaagtttga
     1141 agaagctaaa cctcatctgg aagaccagct ggcaaaatca atcgccaata tactctgata
     1201 taaatatccc ggtggccatt cacctggcct ccggccctct tagtctcaac gttcagacaa
     1261 ttcagatatt attttccctg tcacaccata tacccatgcc ggaacagaat ctaatgacgg
     1321 taccagtaaa ttcttcagcg ttcggtttgc ctgacgcccg tagctgtatt tgtgtctgtt
     1381 tcttcttata caagagcagt agtacaattt ctcttccaga gtcagttcaa ctttaggacg
     1441 taaccaataa agatctcttc cactataaaa tggtgaatcc tgtacaaacg attccataac
     1501 agaacctcct gctgctacag ttaacacagc tgctggcatg gactaatacc atcttttaat
     1561 ttaaccctgg cggagacgcc attatttttg gacgttcttg aaacaaaatt aattcctga
//
LOCUS       DAZFAH010000400         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFAH010000400 DAZFAH010000000
VERSION     DAZFAH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.403"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            357..971
                     /gene="evgA"
                     /locus_tag="ACHJWB_005014"
     CDS             357..971
                     /gene="evgA"
                     /locus_tag="ACHJWB_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4316455.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            976..>1615
                     /locus_tag="ACHJWB_005015"
     CDS             976..>1615
                     /locus_tag="ACHJWB_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4316456.1"
                     /translation="MKFLPYFFLLWCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSN"
BASE COUNT          513 a          320 c          316 g          466 t
ORIGIN      
        1 ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt
       61 aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta
      121 cgcctgtagg attagtaaga agacttatag tgctaacttg aaactataaa tcatcggtac
      181 aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg tatacttgtc
      241 gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct
      301 tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga
      361 acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca
      421 aaaacgatat tgaaatctta gcggagttga ctgaaggggg aagcgccgtt cagcgggtgg
      481 aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc
      541 aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatt gtctccgcta
      601 aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatgga ttcgtgagta
      661 aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct
      721 atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg
      781 actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata
      841 atgacattgc tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc
      901 tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg caacgtaaca
      961 aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctggtg tggtctttgg
     1021 tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac
     1081 cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa
     1141 aaaaatcttg tgattgcagt acacaagtcc caaacggcta cgttgttgca taccgattcg
     1201 cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat
     1261 atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc gcttgcagaa
     1321 ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa taatgacatt
     1381 gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct tcacgactca
     1441 atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa ttacccccca
     1501 gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat
     1561 caggcattag catccgtctc agctgggcac aatgattact ttattggtag taaca
//
LOCUS       DAZFAH010000401         1599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFAH010000401 DAZFAH010000000
VERSION     DAZFAH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.404"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..431)
                     /locus_tag="ACHJWB_005016"
     CDS             complement(<1..431)
                     /locus_tag="ACHJWB_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO4316457.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVAMSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNG"
     gene            complement(803..>1599)
                     /locus_tag="ACHJWB_005017"
     CDS             complement(803..>1599)
                     /locus_tag="ACHJWB_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4316458.1"
                     /translation="EHLRKLTSHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAG
                     TREVQRFRLSGHGCSLVIADLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRA
                     LSVDEYFWRHILHRGHQRVLFVVTQADKTEPCHEWDMAGIQPSPVQAQNIREKTEAVF
                     RLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQARE
                     QFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
BASE COUNT          357 a          473 c          421 g          348 t
ORIGIN      
        1 ccgttcaggc tctgcccccc gccgtcacgg ataaccgtat cactggctgt ccccccctcc
       61 agcacgacct gacgaccatt tgtggtaaca gtggtgtttc cggctatccc gccattctgt
      121 atccattgcc caccggtgtt ttcttcactg tccggcccca gttccagccc ggtactgatg
      181 gtcattccgt tggccgtacc gaagacaatc tggttgtcat gatttgtcag tgttccgccg
      241 ttcacggttt cccctgcctg tacaaccgtg tcagcagcca gtgccgggac tgatgtgaca
      301 gcagcaagag acatcgcaac cgccacaccg gcgcgttttc cccgtgagcg ggccagttcg
      361 gaagccacca ccagggtgcc cgtaatgtga ttccatacca gcctgtagct ggtgttcaga
      421 tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac ccacaaaagt
      481 ggataccggc aggtacaaaa cagagttatc gggcacagcg atacgccccg ggcgtaaacg
      541 ctctcaccct tcatcaggca gagaagtatc agtccgttgt gtgcaattcc gttctgcatg
      601 atgaagagat tcagctgcct tttcgcaggc cattaaccac tataaacgat cgatagtaac
      661 gatcgatacc tgttttatcg atcgttttat tctattaaca gcagagcagt cacgcaagag
      721 aaacaggagg ggcatgaggt gtttgtgtca tgttttcagt tggttaacgc aatgtaatca
      781 ggcatttccc ctggcagagt gttcagaaga aaatccagtt ccatacagcg cgggcaacag
      841 agaccaccga gtcacggacg gcacgcagaa ccgtgcgtgc aacagaggca atacagacgc
      901 tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gcctgagcgc
      961 ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg gcggcatggt
     1021 cgggaagtgc tgtcatgagc gcactgacca gcgtatccag ttcccagccg gtgcgggccg
     1081 atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc gttttctccc
     1141 gaatattctg tgcctgtacg ggagaaggct gaataccggc catatcccat tcatggcagg
     1201 gctccgtttt gtcggcctgc gtcaccacaa acagcacccg ctgatgtccc cggtgcagga
     1261 tgtgtcgcca gaaatactca tccacagaca gggcacggtc atcggcttta atcagccaca
     1321 gtaccaggtc aagttcaggc agaatgtcac ggtacagggc ttcatactct gcatccctgt
     1381 cccggctctc gcccaccccg ggcaggtcag cgataactag gctgcaaccg tggccactca
     1441 gacggaagcg ctgcacttcc cgggtaccgg cgtgaacatc actgaccggg gtgacctccc
     1501 cctgaaacag tgcattacag agtgaggatt taccggcccc gcttttaccc ataatgccaa
     1561 tcacgggttc gtgactggtg agtttgcgca gatgttcca
//
LOCUS       DAZFAH010000402         1591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFAH010000402 DAZFAH010000000
VERSION     DAZFAH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.405"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1160
                     /gene="escC"
                     /locus_tag="ACHJWB_005018"
     CDS             <1..1160
                     /gene="escC"
                     /locus_tag="ACHJWB_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO4316459.1"
                     /translation="TISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQ
                     SKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVE
                     RSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEISVSIIDVDA
                     NDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNALQQNSKAKI
                     LSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDSSNSLT
                     GKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESLLLGGFI
                     QDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            1174..>1591
                     /gene="cesD"
                     /locus_tag="ACHJWB_005019"
     CDS             1174..>1591
                     /gene="cesD"
                     /locus_tag="ACHJWB_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO4316460.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTY"
BASE COUNT          529 a          299 c          314 g          449 t
ORIGIN      
        1 atacaatctc agtaaacaaa aactcttgca atgtgagaaa aattacgaca tttaactcga
       61 ttgaagttcg gggtgttcct gaatgtataa aatatattac atctttatca gaaagtcttg
      121 ataaagaggc acagtctaaa gccaaaaata aagatgttgt taaggtcttt aaactgaact
      181 atgcgtcagc gacagatata acatacaaat accgtgacca aaatgttgtc gtcccaggtg
      241 ttgtctccat tctgaagaca atggcaagta atggttcgct tcccagtacc ggtaaaggtg
      301 ccgtcgagcg aagcggtaac ctgtttgata acagtgtaac gatttccgct gatccacgtc
      361 ttaatgcagt tgttgtaaaa gaccgcgaaa taacgatgga tatctatcag caattaattt
      421 ctgagcttga tattgaacaa aggcaaattg aaatttctgt ctcaattatt gatgttgatg
      481 caaacgatct tcagcagctc ggcgtaaact ggtccggtac gttaaatgca ggacaaggta
      541 cgattgcctt taatagctct accgcacagg ctaatatcag ctcttctgtg ataagtaatg
      601 ccagtaattt tatgattagg gtgaatgcgt tacaacagaa ctcgaaagct aaaatactct
      661 ctcagccgtc aattataacc ttaaataata tgcaggcgat tcttgataaa aacgtaacgt
      721 tttatacaaa agtctccgga gaaaaggtgg cgtctctgga aagtattaca agcggaacat
      781 tgctgagagt gacaccacgt atacttgatg acagctcgaa tagtcttacc ggaaaacgtc
      841 gggaacgagt tcgtttgtta cttgatattc aggatggcaa tcaatcgact aaccaaagca
      901 atgctcagga tgccagcagc accttacctg aagtacaaaa ttcagaaatg acaacagaag
      961 cgacattaag tgcaggagaa agtttattac ttggcggatt tatccaagat aaagaaagtt
     1021 caagtaaaga tggtataccg ctgttaagcg acattcctgt tattggtagt ttattctcca
     1081 gtacggtaaa acaaaaacat agtgttgttc gactcttttt aattaaagca accccgataa
     1141 aatctgcatc tagcgaataa taaatgaggc tttatgagca ggaaatttag ctctctagag
     1201 gatatttatg atttctacca ggatggtggc acattagcgt cattaacaaa tctgacacaa
     1261 caagatctca atgaccttca ttcttatgcc tatacagcat atcaatctgg tgatgttata
     1321 accgcaagaa atctattcca tttgctcaca tatctggaac actggaatta tgactacacc
     1381 ttatctctgg gcttatgcca tcagcgttta tcaaatcatg aagatgcaca actgtgtttc
     1441 gcacgctgtg caactttagt tatgcaagat cccagggcat cttattattc tggaattagc
     1501 tacttactcg tcggaaataa gaaaatggcc aagaaagcct ttaaggcttg tttaatgtgg
     1561 tgtaatgaaa aagaaaaata cactacatat a
//
LOCUS       DAZFAH010000403         1583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFAH010000403 DAZFAH010000000
VERSION     DAZFAH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.406"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            266..1255
                     /locus_tag="ACHJWB_005020"
                     /pseudo
     CDS             266..1255
                     /locus_tag="ACHJWB_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1338..1583
                     /locus_tag="ACHJWB_005021"
                     /pseudo
     CDS             1338..1583
                     /locus_tag="ACHJWB_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          457 a          368 c          408 g          350 t
ORIGIN      
        1 gaattattgt taaaaaacgc tttgttgaat caaccgaact gttgctgagt tgcaggatca
       61 catcacgtat tttcctgaaa acgcaggctg cttagtagca aagcagaagt tcaaaatcac
      121 caactaaata gctgcgccga atagtagatc aatgaaaggg aactcagccc ggattgtgcg
      181 atctgatcaa tcgccaaaaa tcccccccta accggactga gcgatgccga tcatagcacc
      241 aattccccgc gacgaatgac gcctgatgca aaaagcaatc tataaaactc acgataaaaa
      301 ttatgcccgc aggctgaccg ccatgctgat gctgcaccgg agcgctcgcg tcagtgacgt
      361 tgccagaacg ctctgtttcg cccgttcatc tgtcgggcgc tggattaact ggttcacaca
      421 gtcgggtgtt gatggactga aatcattacc cgccgggcgt gcccgccgct ggtcatttga
      481 acatatctgt acactgttac gtgagctggt aaaacattct cctggcgact ttggctacca
      541 gcgttcacgc tgaagcacag aacggttggc aataaaaatc aatgagataa ccggatgtca
      601 gttacatgcc ggaaccgtgc gccgctggtt gtcgtctgcg gggctggtgt ggcgcagagc
      661 agcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggtgg caattcataa
      721 agcactggac gaatgcagcc cagagcatcc ggtattttat gaagatgaag tggatatcca
      781 ccctaatccc aaaatcggtg cgggctggca gcttcgcgga cagcaaaaac acgtggtgac
      841 gccgggtcag aatgaaaaat attatctggc tggcgcgtta cacagcggaa cgggaaaagt
      901 gagttatgtg ggtggcaata gcaagagctc cactttattt atcagtctgt tgaaacacct
      961 gaaatcgaca taccgacggt ctaaaactat catgctcatc gtggacaact acatcatcca
     1021 caaaagccgg gaaacacagc gctggctgaa gcagaatccg aagttcaggg tgatttatca
     1081 gctggtttac tctccatggg tgaatcacgt tgaacggcta tggcaggcgc ttcacgacac
     1141 aataacgcac aaccatcagt gccgttcaat gtggtagttg ctgaaaaaga ttcaccattt
     1201 tatgggaacc gtcagcccat tccccggagg taattatgga caggcaaaag tgtagcggta
     1261 ttaggcacag ctatttagta ttaagactat cacttattta agtgatattg gttgtctgga
     1321 aataaggggg caagaacaag tggctgacgg ataatggttc atgctaccgg gcgaatgaaa
     1381 cacgtcagtt cgccaggatg ttgggacttg aaccgaagaa cacggcagtg cggagtccgg
     1441 agagtagcgg aatagcagag agcttcgtga aaacgataaa gcgtgattac ataagtatca
     1501 tgcccaaacc agacgggtta acggcagcaa agaaccttgc agaggcgttc gagcattata
     1561 acgaatggca tccgcatagt gca
//
LOCUS       DAZFAH010000404         1576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFAH010000404 DAZFAH010000000
VERSION     DAZFAH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.407"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..1554
                     /locus_tag="ACHJWB_005022"
     CDS             148..1554
                     /locus_tag="ACHJWB_005022"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO4316461.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKESLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQDEDNFIAAILAAKNFAEQGKLVTFGIIPTSPETGYGYIKSGES
                     LDGQGYKVAAFVEKPDLHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDSASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLIVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
BASE COUNT          444 a          295 c          346 g          491 t
ORIGIN      
        1 tggatatgaa atccaactta aacctcagca acttcaccta ccaacggtcc agcataattc
       61 ctacaagtgg tttgatgtag taacgttgct taatagcact atagttcatc aatataccaa
      121 aaactatttt ataccaaggt aatagatatg ctacttcccg ttgtcatggc cggtggttct
      181 ggtaccagat tatggcctct ttcacgtaca ctttatccga aacaatttct gtctttaaat
      241 agtcgtttaa ccatgttgca agagacattg cggcggcttg ataaggtcga acataaacct
      301 gctttggtca tttgtaacga atcacatcgc tttatcgttg ctgaacaatt gcgtaaagag
      361 agtttaaagc atagcggtat tttgcttgag cctgttggtc gtaatactgc gcctgctgta
      421 gcacttgcag cacttcaggc tatggtaact ggagacgatc ctattctttt ggttcttgct
      481 gctgatcatg aaatccagga tgaggataat ttcattgctg caattctcgc tgcaaagaat
      541 tttgcagagc agggtaagct tgttacgttt ggtattattc caacatcccc agagactggc
      601 tatggttaca ttaagtcagg tgaatctctg gatggacaag gttacaaagt tgcggctttt
      661 gttgaaaaac cagatcttca cgtagctcag cggtacatat cagatggcgg ttatctttgg
      721 aatagcggga tgtttatgtt cagagcatct gtatttatcg atgaactgaa aaaattccga
      781 ccagatattt tagccagttg ccaacgctcc ttgtcctctt cgatacaaga tttagatttt
      841 atccgtctgg atagtgcttc attttcttgc tgtcctgaag agtctattga ctatgcagtt
      901 atggaaaaaa cagccgaagc tgtcgtcgtt ccattaaatg cacaatggag tgatgttggg
      961 tcatggtctg cattgtggga aataagttca aaagatcaaa gcggtaatgc cattcgtggt
     1021 gatgtattgg ttgaagatgc tacagatagt tatctttatt cgcagcatag acttattggt
     1081 gccgtgggcg taaaggattt gattgttgtt gaaacgaaag atgccgtatt agttgctcat
     1141 aaagataaag ttcagcaagt taaaaacatc gtcgctcaac ttaaaaagaa taatcgcaca
     1201 gaatatttac agcatcggga aatatttcgg ccttggggca gtcatgatac tatagctgaa
     1261 gggccgcgct ttcaagtgaa acatgtgatt gtattgcctg gtcacattac cgctaagcag
     1321 attcattacc atcgtactga acattggatt gttgtctcgg ggacagctaa agttcatctt
     1381 gaagataaga cttaccttgt ttctgaaaat gaatcaacat atatacctgt tggtgttcca
     1441 catgccattg aaaatcctgg caagatcccg cttgaaataa ttgaggttag atcaggagtc
     1501 tacctggaag aggatgatgt tataagagtt tcttcttctg gagtaggata ctaatgcgaa
     1561 tttctattat cacagc
//
LOCUS       DAZFAH010000405         1569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFAH010000405 DAZFAH010000000
VERSION     DAZFAH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.408"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..884
                     /gene="yiaJ"
                     /locus_tag="ACHJWB_005023"
     CDS             36..884
                     /gene="yiaJ"
                     /locus_tag="ACHJWB_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO4316462.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            986..1457
                     /gene="ysaA"
                     /locus_tag="ACHJWB_005024"
                     /pseudo
     CDS             986..1457
                     /gene="ysaA"
                     /locus_tag="ACHJWB_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
BASE COUNT          378 a          384 c          433 g          374 t
ORIGIN      
        1 cggcacactt tgcgctacca tcaggacgcg acaaaatggg gaaagaagtg atgggaaaaa
       61 aagagaacga gatggcgcag gaaaaagagc gtccagccgg aagccagagt ctgtttcgcg
      121 ggttgatgct gattgagatt ttgagcaact atccaaacgg ttgtccgttg gcgcatcttt
      181 cggagctggc tggtttaaat aagagtaccg tccatcgctt attgcaggga ttacagtcct
      241 gcggctatgt gaccaccgcg cccgccgcag ggagttaccg cctgactacc aaatttattg
      301 ccgtcgggca gaaggcgctg tcttcgctga atatcattca tatcgccgct ccgcatcttg
      361 aggcactgaa catcgccact ggtgaaacca ttaacttctc cagccgcgaa gacgatcacg
      421 ctattttgat ttataagctg gaacccacaa ccgggatgct gcgaacccgt gcctatattg
      481 gccagcatat gccgctctac tgttccgcaa tgggcaagat ctacatggcg tttggtcacc
      541 cggactacgt gaagtcatac tgggaaaacc atcagcatga gatccagccg ttaacccgca
      601 ataccattac cgagctgccc gcgatgttcg acgaactggc gcacattcgt gaaagcggag
      661 cggcgatgga cagagaagaa aacgaactcg gcgtctcctg tattgctgtt ccggtgttta
      721 atattcatgg gcgggtgccg tacgccgtgt cgatttcgct ttcgacatca cgtctgaaac
      781 aggtggggga gaaaaatctc ctgaaaccac tgcgtgaaac cgcgcaggct atttctaatg
      841 aactgggatt tactgtcagg gacgaccagg gcgcgatcac ataacgcttt ggacaagtgc
      901 caaaacttta acatttcctt cgttggatca aagcagtagg gacgcgctct ctggcactct
      961 gctgttttag tgcaaaggag tgatcatgaa ccggtttatt attgcggatg cgacgaaatg
     1021 tatcggttgc cgtacctgtg aagtggcttg cgcagtgtcg catcaggaga atcaggattg
     1081 cgctgcgttg tcaccagacg agtttatttc ccgtattcgt gtcattaaag accacagctg
     1141 gaccacggca gtagcctgtc atcagtgtga atgcaccgtg cgcgaatgtc tgccctgttg
     1201 acgcgataag ccgcgaacat gggcatattt tcgttgaaca aacacgttgc attggctgta
     1261 aaagctgtat gctggcttgc ccgtttggtg cgatggaggt cgtttcttcg cgcaaaaagg
     1321 cgagggcgat taagtgcgat ctgtgctggc atcgggagac ggggccggcc tgtgttgaag
     1381 cctgcccgac aaaggcgttg cagtgcatgg atgtcgagaa agtgcagcgg catcggctac
     1441 ggcagcagcc tgtttgaaac gttttccggc gtttctcaac gccggaatag tgcgctttgt
     1501 ttatgccaga tgcggcgtaa acgccttatc cggcctacaa attcttccaa attcaatata
     1561 ttgcagaaa
//
LOCUS       DAZFAH010000406         1562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFAH010000406 DAZFAH010000000
VERSION     DAZFAH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.409"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            622..849
                     /gene="ykgL"
                     /locus_tag="ACHJWB_005025"
     CDS             622..849
                     /gene="ykgL"
                     /locus_tag="ACHJWB_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO4316463.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(884..1024)
                     /gene="ykgO"
                     /locus_tag="ACHJWB_005026"
     CDS             complement(884..1024)
                     /gene="ykgO"
                     /locus_tag="ACHJWB_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO4316464.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1024..1287)
                     /gene="ykgM"
                     /locus_tag="ACHJWB_005027"
     CDS             complement(1024..1287)
                     /gene="ykgM"
                     /locus_tag="ACHJWB_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4316465.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          462 a          292 c          354 g          454 t
ORIGIN      
        1 gtcaatatgt gaacggaaaa ggaattcaat ggttaagtca atatttagaa aaactgaaaa
       61 tattgctttt ttgataagaa gaaaaagaca tttggtttca taaggttatt ggcaatataa
      121 cgttcaattc ttacttgcat tttatattgg tcaggaactt tgccctgaag tgttaggaat
      181 agtcttaatg cgctgtgtga tggactagta aaaaaacaag ttttaagggt aagggataga
      241 atataaaact ataatgagct gtcattgaag atgatttgcc aatcagtaag aaattttaat
      301 ggattaacat gccgcacaaa atggttatag gtgttcatta caataagtga tggaaaattg
      361 aacgggcgta tgtacgtcat ctgttcggta agcccccaag agccagatta agtgaagtta
      421 aacgaaggct gccagctgtc acaggaacga tggcctcttt gtcagggcac tgagtgcagt
      481 tatctgtgcg cgttactcta actggcactc gaacgtcggc gacggggtta ccggtggatc
      541 agactgttcc tgtggcgtaa acgtttttat gttagtacca acagatttac cacatttatc
      601 atcttaacag tttgagtgaa aatgcaggct gcgccgcaaa gggaggagac tgagtggcgt
      661 gttcagtcta aaagagagtt gatgccagca tacaggggcg aagctggtca acaagtcaac
      721 attaagataa tggaatattc ggagagaaac gtcaggcagt tggcctcaaa tgaacaggaa
      781 gaatatatcc ccaggaaaat taatgttggt gttataaaca caccaacatt aatccgatct
      841 gattattaat cagacaaata tcgttaatcc gtcgaatttt gaatcaacgt tttttcttac
      901 gaccctgaac ggccttaaaa cgtggattag atttacaaat cacatatagc cgtccttttc
      961 gcttcacaat ctgacagtct ggatggcgtt cttttgcggt acgcagagag ttaaggactt
     1021 tcatcatgcc ccctttttcg tgctaacaaa acgaccaaaa cgttgggtga accgtgcaac
     1081 atttccttct gatgccactg ttctcagctt ccctgtatag aacgggtgcg atttcgaaga
     1141 gacatcaatt gttacgtatg gatacgttac gccatccagc tcaatctcac ggtctgtttt
     1201 gatagtcgag ccgattttaa agtactcatc aatactggtg tcgtggaaca ccacagtacg
     1261 atactcagga tggatattgg gcttcatcat ttttacctgt tatgttataa cataaccata
     1321 aagatacatg ctcatccgga tcatttcaac acccaatccc tgtgaccatt agtggtatgt
     1381 ggaatttccg caacggggaa gtctggcatt gttagcagcc attggtgacg ctcccataaa
     1441 cgcctggatg agtgattgat gatggtttcg ctgagagtat ggatttttcg agaatggagg
     1501 ttatctccac tttactggcc gatgatttat cgcagtatga ttgattacct gacagcgtgt
     1561 at
//
LOCUS       DAZFAH010000407         1559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFAH010000407 DAZFAH010000000
VERSION     DAZFAH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.410"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHJWB_005028"
     CDS             <1..112
                     /locus_tag="ACHJWB_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4316466.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            180..422
                     /locus_tag="ACHJWB_005029"
     CDS             180..422
                     /locus_tag="ACHJWB_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4316467.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            400..1419
                     /locus_tag="ACHJWB_005030"
     CDS             400..1419
                     /locus_tag="ACHJWB_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4316468.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
BASE COUNT          517 a          328 c          352 g          362 t
ORIGIN      
        1 tgctatccca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt atcaggcaaa
       61 atacgtttca gctatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 ccatatcgcc gcccaccagc tacagtggcg gcggtcatgc tgtaaaggca cgtgaccaca
      181 tgtacgaatt aactctatct ccagcagaga ttaaagagat cacgaaatac gagcgataca
      241 caaaacagca acaccagtta agactgcacg gcatcccatt tgtaatcggt cctaaaaacg
      301 aaccaatagt tctacgcagg gatattccac acggactgac aacgatgcca aaaacatctg
      361 aactggtttc tgctgaaccc gattttgagg cgctgaataa tgggaagacc aagaaaaaat
      421 aaaaaagata atgtactgcc accgcgggtt agatcgaatg gttacagtta cgtgtggaaa
      481 ccagaaggaa gtacaagaac tatagggcta ggaagagtgc ggaaaaccag cgtagctaaa
      541 gtctggcaaa attatgaact ggaaaaagca aaactccaca acataatgac cgtagctaaa
      601 ttatggcaca tgtttatgga ctcccctgca tttacagaac tggccccccg aacccaaaaa
      661 gattatcgac aacatcagaa ggcgttgctg atggtattcg gaaaagtgct tgctgataat
      721 gtcaaaactg agcaggtaag aattttcatg gataaacgag ggcttgagag caagacccag
      781 gcaaatcatg aactggcaag cctgagtcga gtatacgggt ggggatatga gcgtgggtat
      841 gtgaagaata acccatgcaa aggagtcaga aaattctctc ttaaagcccg cactgtttac
      901 atcaccgatg aacagtatgc ggcgatatat gcggaagcaa ttccacagtt acgcattgca
      961 atggagattt cctatctctg tgcggcaaga ctcggtgatg tgcttgagtt gaaatggcag
     1021 gatattatgg ataaagggat ctacattgag caaaacaaaa ccggcaccaa acaaatcaag
     1081 gaatggtcac cgcgattacg tacagcgatc cagttagccc gaaatgtatc ttcctgtaca
     1141 tgcgaatatg tgatcaatac aaccaaaggc gggaaagtca tagctaaaac gctgaataac
     1201 tggtggaatc aggctaaacg cgcagccgag caaaaagttg gcgttccgtt cgggtgcaat
     1261 tttcacgaca taaaagccaa ggggatctca gattacgaag gcagcagtcg cgacaaacaa
     1321 attttcagcg ggcataaaac agaaaatcag gtgttgattt acgatcgtaa aacaaaaatc
     1381 acaccaacac tggatttgcc gctcgtggtt agcaagtaga caatccagaa aaatttctgg
     1441 caaaaagact tggtatacta gctatgagca acttcctaat accttgattt ttcttggtgt
     1501 taaatttgat taacttttca gcaatttcac gtattgcact gatttttatt tgtttttct
//
LOCUS       DAZFAH010000408         1520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFAH010000408 DAZFAH010000000
VERSION     DAZFAH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.411"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1520
                     /locus_tag="ACHJWB_005031"
                     /pseudo
     CDS             1..1520
                     /locus_tag="ACHJWB_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
BASE COUNT          414 a          362 c          438 g          306 t
ORIGIN      
        1 tggttcgtct tgctgcggca atggggactg aatttccttt tgatgaatac gcagaagtgc
       61 agaaactgcc ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg
      121 gaacactcac actgctggat acccccggcc ctaatgaggc cggacaaccg cagatggaag
      181 tgatgatgcg ggatcaactg cagaaagcct ctgcggttct ggctgtgatg gattacaccc
      241 agatgaactc aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat
      301 cagccggccg cctgtttgta ctggtcaata aatttgatga gaaagaccgc aatggcgatg
      361 gggcagatgc cgtacgccag aaagttccgg caatgctgaa cagcgatgtg ctgcccgcct
      421 cccgcgttta tcccggatcc tcacgccagg catacctggc taaccgtgcg cttcatgagt
      481 tacggaaaaa cggaaccctt cctgttgatg aagcctgggt cgatgatttt atcagaggtg
      541 ccttcggttg catgaaaaaa gaatacgtct gtaaagacag tgaactggca actgaagggg
      601 cgacagacct gtgggaaggc tcacttatcg atcaactgat aacggaagtc atacagagct
      661 cacattccag agctgcggca ctggcggttg actctgccgc cgcaaaactg atgcagaatg
      721 cagaaaatat cagtgaatac ctgttgttac gccatcaggg gctacagcaa agcattcagt
      781 cactgcagtc gcatatcacc agcctgcttg cggatatccg ggaaatcgcg gactgtcagg
      841 agcagatgac cactgatgtc agaatggcca tggaggagat cgatactaaa acccgggaat
      901 tactgacggg gtctgcacct cactggaaga ggagctgaat gactatttca gaagcggtaa
      961 acgcaaagaa cagcaaatgc tggaggaaga agacgcagaa caacgaaggt ctcagtccgg
     1021 attatggggg aaaatttctc aatggtccgg tatcaacaac cagggccggg aggattacag
     1081 gaaacgagat tttgccccgg acagccccga aataaaattc agtgatcgca gggaagccct
     1141 tgaactgatg acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca
     1201 gttccggcct gagctggaga aaatcgtcag cgggattgaa acaggttttc gtggcacggc
     1261 cctgtacgcc acagaaaaca ttgccggtcg catcaatgcc cgcctggagg atgagggctt
     1321 taccgtaaaa atcagttttc cggcagtcag ccagttacag acccggctcg cggtaaaaat
     1381 aaatctgagt gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag
     1441 tggcgtatgg ggaaccgttt gtcgatggtt tggcaccagt gacctgggct gggaaaacta
     1501 tgacgaggat gtgagtcgca
//
LOCUS       DAZFAH010000409         1513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFAH010000409 DAZFAH010000000
VERSION     DAZFAH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.412"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..665
                     /locus_tag="ACHJWB_005032"
     CDS             <1..665
                     /locus_tag="ACHJWB_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4316469.1"
                     /translation="EKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALA
                     TGLRKSNIINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKW
                     VFVHTKAAKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQS
                     GVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIK
                     KA"
     gene            complement(681..757)
                     /locus_tag="ACHJWB_005033"
     tRNA            complement(681..757)
                     /locus_tag="ACHJWB_005033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(721..723),aa:Arg,seq:tct)
     gene            1000..>1513
                     /gene="fimZ"
                     /locus_tag="ACHJWB_005034"
     CDS             1000..>1513
                     /gene="fimZ"
                     /locus_tag="ACHJWB_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4316470.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKNNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIA"
BASE COUNT          449 a          324 c          356 g          384 t
ORIGIN      
        1 tggaaaaagc gcctgtcatc aagataccag cggtcagaaa caagcgagtc agatggctgg
       61 aaaaggagga agcaaaacgc cttattgatg agtgccccga accactgaaa tctgtcgtca
      121 agtttgcgct ggcaactggt ctgagaaagt cgaacatcat aaatctggaa tggcaacaaa
      181 tcgacatgca gcgacgagtt gcctgggtga atccagaaga gagcaaatca aaccgcgcca
      241 ttggtgtggc gctgaacgat accgcctgta aagtgttgcg tgatcaaata ggcaagcatc
      301 acaaatgggt gtttgtacat accaaggcgg ctaagcgagc agatggaaca tcaacgcctg
      361 cggtcaggaa gatgcgcatc gacagcaaga catcatggct atcagcttgt cgtcgtgcag
      421 gaattgaaga tttccgtttc catgacctca gacacacctg ggcaagctgg ctgatccagt
      481 caggcgtccc attatctgtg cttcaggaaa tgggcggatg ggagtccata gaaatggttc
      541 gtaggtatgc tcaccttgcg cctaatcatt tgacagagca tgcaaggaaa atagacgaca
      601 tttttggtga taatgtccca aatatgtccc actgtggaat tatggaggat ataaagaagg
      661 cgtaactgat tgaattgtaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt
      721 agaaggtcgt tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa
      781 tcgcgtgaat tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac
      841 aagtgaacga ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca
      901 ccgctgcgcg tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc
      961 cctaacggaa aattgtccgc tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc
     1021 attatggata ctcatcctat catcagaatg tctattgaag ttctgttgca aaaaaacagt
     1081 gaattgcaga ttgtcctgaa aacggatgat taccgcataa ccatcgatta tctccgaacc
     1141 cgtcctgttg atttaatcat tatggatata gacttgcccg gaacagacgg ttttaccttc
     1201 ctgaaaagga tcaaacaaat ccagagcaca gtgaaagtgt tatttttatc atcgaaatca
     1261 gaatgctttt atgctggcag agcgatacaa gctggtgcta acggttttgt cagtaaatgc
     1321 aatgatcaga atgatatttt tcatgccgtt cagatgatcc tctccggata cacgtttttt
     1381 cccagcgaaa cgcttaacta tataaaaaac aataaatgta gtacgaatag ttcaacggtc
     1441 actgtgctat ctaatcgtga agtgaccata ttacgttatc tggttagcgg attatctaat
     1501 aaagaaattg ccg
//
LOCUS       DAZFAH010000410         1504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFAH010000410 DAZFAH010000000
VERSION     DAZFAH010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.413"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..750)
                     /locus_tag="ACHJWB_005035"
                     /pseudo
     CDS             complement(26..750)
                     /locus_tag="ACHJWB_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(747..1078)
                     /gene="sctI"
                     /locus_tag="ACHJWB_005036"
                     /pseudo
     CDS             complement(747..1078)
                     /gene="sctI"
                     /locus_tag="ACHJWB_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1098..1338)
                     /locus_tag="ACHJWB_005037"
                     /pseudo
     CDS             complement(1098..1338)
                     /locus_tag="ACHJWB_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1352..>1504)
                     /locus_tag="ACHJWB_005038"
     CDS             complement(1352..>1504)
                     /locus_tag="ACHJWB_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4316471.1"
                     /translation="NYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGS
                     NWTFQE"
BASE COUNT          447 a          307 c          247 g          503 t
ORIGIN      
        1 caagattcat aacgctctca tatgtctact taattttcaa cctgactaaa ttagttagaa
       61 tggccctata cttccataac agccagcaag tcgctacgga tattaatgca agtaagatag
      121 aaaccggcat agccttatca taagcaaaaa caggttcgct aatttcatat gttggtgctt
      181 gctcaataat gtctcttcgt tttgacaata caacagaaat attttcatat tgtacgcttg
      241 cagagctatt aacaataaat ctcttgatat catttatttt tatttctggg ttgatatctt
      301 tttcatatac tgcaagtaca gaaatatgaa ttggtaaagc agttttacca ctatcgccat
      361 tatcaacatc gtaactaaca tgtactctcg aagaaataat gccatccata attttgagag
      421 attgctctaa ccgctgctca atagcagaat atagccttgc tttttccgct cgtggagatg
      481 aaacgagtgc atctgcaggg aacatctgcg atatttgaat atcaggctta cccggtagat
      541 tgtagatttt tagccaatcc accgcagaag caaaatccgt tggttcgaca aatattgaaa
      601 atcctgtttt gccttgatcc ttcttttcag cattaatatt atgtctttgt aaaacagcaa
      661 ggacatcagt tgatcaagat ggttcaataa ttcctgctgc ttgcagccgc acaacagcag
      721 gataaacaat aataatgata tatatttcat gagcgggtca acgtctcaac tgcagcgaca
      781 atctttcttg ctaatgttcc accaatgaaa cctcgacatt ataattaagc acatgatcct
      841 gcaagactaa taatttttca ggagttaacc cctctttatc agaccttaat gcttgtaacg
      901 cattttgctg ccaattttca gtttgtacag catagttggc aaaagcggag atcattttat
      961 catccgggtt gtcaagattg agaacccggt catttaaatc aatcactttc gcttgctgta
     1021 tttcatgagc agttgattgc tcagctctat caataggtgg catattgctt actgacataa
     1081 taaaatcctt atccagttta gcggaagttt tgtatgattg ctgaatcaag atctttatac
     1141 gcttttacaa cgttagattg cgcatttcga tataatgtat actcagctaa tgcactttga
     1201 tattcggcaa gaaatttcgt ttcggaggga ttggttggct aaatcctcca acgctttttc
     1261 aacttgctgg ttcagatcat caacgccctg atcaaattga ttgctgatat ccataatata
     1321 accattccaa tctgccataa gtcctcctca attattcctg aaatgtccag ttgctacctt
     1381 gagacttctc gaatcggtta tctccgtagc taaatgaact attatttgtg tcctgatttt
     1441 tcaaattcac attaaatctt acatattgtt taccccattg tttataatat tcgtcaacat
     1501 agtt
//
LOCUS       DAZFAH010000411         1492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFAH010000411 DAZFAH010000000
VERSION     DAZFAH010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.414"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1467
                     /locus_tag="ACHJWB_005039"
     CDS             <1..1467
                     /locus_tag="ACHJWB_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFO4316472.1"
                     /translation="QRAGFFPEEATWSGLVPSLVREYRYDSAGNLSAVTSREDYGRET
                     RREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQF
                     SRVRQAHPSVAPQWVTRTNYAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TGYPDPRGELDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPLGYVDPLGLAVCPKLYEIYKGLRAQGFNPAEARDLAKWFNSSWGKADVY
                     LRSAIGSGKIDYVGISRNVLNRYPQGKYDLELLTRLTGQLPRNQARAIEQAIINAKRG
                     NGFTNIQNSIDPAKDHFDAAVKWGEEWLKTNGFGGWLQ"
BASE COUNT          392 a          340 c          476 g          284 t
ORIGIN      
        1 cagcgcgccg gattttttcc ggaagaagcc acatggagcg ggctggttcc gtcactggta
       61 cgggagtacc gttacgacag cgcgggtaat ttgtcggctg tcaccagccg ggaagactac
      121 ggacgggaaa cacggcggga gtaccggctg gaccggaacg gtcaggtcac ggcggtgaca
      181 gcctcaggca ccgggctggg ctatggcgaa ggcgatgagt cctatggcta tgacagttgt
      241 ggctacctga aggcgcagtc tgcgggcagg caccggataa gtgaagagac tgaccagtat
      301 gccggaggcc accggctgaa acaggccgga aacacgcagt atgactatga cgccgcaggc
      361 cggatggtca gccgcaccag acaccgtgac ggctatcgcc cggaaacaga gcggttccgg
      421 tgggacagcc gtgaccagct gaccgggtat tgcagcgcac agggtgagca gtgggaatac
      481 cgccacgacg ccagcggcag acgaacagag aaacgctgcg accggaagaa aatccgcttc
      541 acgtatctgt gggacggcga cagtattgcg gaaatccggg aataccgcga tgataaactg
      601 tacagcgtac ggcacctggt gtttaacggc tttgagctga taagccagca gttcagccgg
      661 gtacggcagg cgcatccgtc ggtggccccg cagtgggtga cgcgaacgaa ttatgcggtg
      721 agcgacctga cgggccgtcc gctgatgctc tttaacagtg aaggtaaaac cgtctggcga
      781 ccggggcaga ccagcctgtg ggggctggca ctcagtctgc ccgcagacac cggctacccg
      841 gacccgcgcg gggaactgga cgcggaagcc gaccccggcc tgctgtatgc gggacagtgg
      901 caggatgcgg aatccgggct gtgctataac cggttccggt attacgagcc ggaaaccggg
      961 atgtacctgg tgagtgatcc gctggggtta caggggggtg agcagactta ccggtatgtg
     1021 cctaatccgc tggggtatgt tgatccgtta gggttggctg tatgtcctaa attgtatgaa
     1081 atatacaagg gattaagagc acaaggattt aacccagcag aagctagaga tctagcaaaa
     1141 tggttcaata gtagctgggg aaaggctgat gtatatcttc gttcagcaat aggatcagga
     1201 aaaatagatt atgttggaat aagtagaaat gtgcttaatc gttatccgca aggtaaatat
     1261 gatttggagt tactcacaag actaacagga caacttccga ggaatcaagc aagagcaata
     1321 gaacaagcta tcattaatgc taaaagagga aacggcttca caaatatcca gaatagtata
     1381 gatcctgcta aagatcattt tgatgcagcg gtaaaatggg gtgaggagtg gttaaagact
     1441 aatggatttg gtggatggct gcaataaaaa tgggggtatt aatgaataac ca
//
LOCUS       DAZFAH010000412         1484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFAH010000412 DAZFAH010000000
VERSION     DAZFAH010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.415"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..738
                     /locus_tag="ACHJWB_005040"
     CDS             <1..738
                     /locus_tag="ACHJWB_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4316473.1"
                     /translation="AYGYAIVNKTFEGTVAINRDDFEDDNLGIYSPLFQEMGRAAAAQ
                     PDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQA
                     DYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGY
                     GFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQLLE
                     RELFADGNATVSNEMKGKLELVVADYL"
     gene            782..1150
                     /locus_tag="ACHJWB_005041"
     CDS             782..1150
                     /locus_tag="ACHJWB_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4316474.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEQDAPRKKTGNKAEQARA"
     gene            1147..>1484
                     /locus_tag="ACHJWB_005042"
     CDS             1147..>1484
                     /locus_tag="ACHJWB_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4316475.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNG"
BASE COUNT          313 a          396 c          450 g          325 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaacc tttgaaggca ccgtcgccat taaccgcgat
       61 gacttcgaag acgataacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gctgcggcac aaccggatga actggtcttt gctgctcttc gtgacgggat taatgcggcc
      181 tgctatgacg gccagaactt ctttgatacc gaacatcctg tttaccccaa agtggatggc
      241 tccggggatg cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggtgca
      301 caggcggatt acagcggtcc tgcctggtat ctgctcgact gttcacgcgc aatcaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt
      421 cgcgcattca ccgataacga atttgtgttc ggtgcttccg cccgtcgcaa tgtgggctac
      481 ggcttctggc agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac
      541 ggctggtcag ccatgcgcga atttaccgct gacggtggcc gcaaactcgg cattaaaccc
      601 acccatattg ttgtccccac ctcgctggaa aaacaggcgg tgcagttgct ggagcgtgaa
      661 ctgttcgcag acggaaacgc aaccgtctct aacgagatga agggcaagct ggagctggtt
      721 gtcgcggatt atctgtaaac ggtgagccgg gtttacggcc cggcccctgc ggagggcaaa
      781 tatgaatgaa catcacactg tggcagcaac agatgattca ggtcttcagg tcagtggtga
      841 caattctgtc acggtgctgg cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg
      901 gttcctgttc acgcgtggac gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact
      961 ggaggaggag ccatgcctca ctgtgcgaat actacagacg tctgctgatg atgcggggag
     1021 cgtggcgggt gtggttcatg cagtggccgg tacagattta gccgaagccg aagccgaagc
     1081 cgaagccgaa gccgaacagg atgctccccg gaaaaagacc ggtaacaaag cggaacaggc
     1141 ccgtgcatga attacgccac tgaaaccgat atgcgggcgc gttaccgcga ggatttgctc
     1201 aggccgttac tggctgtgcc ccgttcggat gagccggaca cgcgcaagct gaaccgggcg
     1261 ctgacagatg cgtcagccct tatcgacagc tatctgtccg cccgttacac gttgccgctg
     1321 gaggttattc cggccgttct tgttcagcac tgttgcgcga ttgcctttta ttacctgtgc
     1381 gatcagcgag cctccgatca ggcgcgtgac cgttaccgtg aagcgctggc ctggctgaaa
     1441 gatgtcatga acggcaatgt gccggtcggt gtggatacga acgg
//
LOCUS       DAZFAH010000413         1472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFAH010000413 DAZFAH010000000
VERSION     DAZFAH010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.416"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..738
                     /locus_tag="ACHJWB_005043"
     CDS             <1..738
                     /locus_tag="ACHJWB_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4316476.1"
                     /translation="AYGYAIVNKTFEGTVAISRDDFEDDNLGIYSPLFQEMGRAAAVQ
                     PDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQA
                     DYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGY
                     GFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQLLE
                     RELFADGNATVSNEMKGKLELVVADYL"
     gene            782..1138
                     /locus_tag="ACHJWB_005044"
     CDS             782..1138
                     /locus_tag="ACHJWB_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4316477.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAGQ
                     DAPWKKTGNKAKQARA"
     gene            1135..>1472
                     /locus_tag="ACHJWB_005045"
     CDS             1135..>1472
                     /locus_tag="ACHJWB_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4316478.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNG"
BASE COUNT          304 a          391 c          456 g          321 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaact tttgaaggca ctgtcgccat tagccgcgat
       61 gacttcgaag acgacaacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gcggcggtac aaccggatga gctggtcttt gctgccctgc gtgacgggat cagtgcggcc
      181 tgctatgacg gtcagaactt ctttgatacc gaacatccgg tttaccccaa agtggatggc
      241 tccggggatg cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca
      301 caggctgatt acagcggtcc ggcctggtat ctgcttgact gctcccgcgc ggtaaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggc
      421 cgcgcattca ccgataacga atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac
      481 ggcttctggc agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac
      541 ggctggtcag ccatgcgcga atttaccgct gacggtggcc gcaaactcgg cattaaaccc
      601 acccatattg ttgtccccac ctcgctggaa aaacaggcgg tgcagctgct ggagcgtgaa
      661 ctgttcgcag acggaaacgc aaccgtctct aacgagatga agggcaagct ggagctggtt
      721 gtcgcggatt atctgtaaac ggtgagccgg gcttgcggcc cggcccctgc ggagggcaaa
      781 tatgaatgaa catcacactg tggcagcaac agatgattca ggtcttcagg tcagtggtga
      841 caattctgtc acggtgctgg cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg
      901 gtttctgttc acgcgtggac gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact
      961 ggaggcggag ccatgcctca ctgtgcgaat actacagacg tctgctgatg atgcggggag
     1021 cgtggcgggt gtggttcatg cagtggccgg tacagattta gccgaagccg aagccgaagc
     1081 cggacaggat gctccctgga aaaagaccgg taacaaagcg aaacaggccc gtgcatgaat
     1141 tacgccactg aaaccgatat gcgggcgcgt taccgcgagg atttgctcag gccgttactg
     1201 gctgtgcccc gttcggatga gccggatacg cgcaagctga accgggcgct gacagatgcg
     1261 tcagccctta tcgacagcta tctgtccgcc cgttacacgc tgccgctgga ggttattccg
     1321 gccgttcttg ttcagcactg ttgcgcgatt gccttttatt acctgtgcga tcagcgagcc
     1381 tcagatcagg cgcgtgaccg ttaccgtgag gcgctggcct ggctgaaaga tgtcatgaac
     1441 ggcaatgtgc cggtaggtgt ggatacgaac gg
//
LOCUS       DAZFAH010000414         1470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFAH010000414 DAZFAH010000000
VERSION     DAZFAH010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.417"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..229)
                     /locus_tag="ACHJWB_005046"
     CDS             complement(32..229)
                     /locus_tag="ACHJWB_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4316479.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRIQS"
     gene            complement(454..1008)
                     /locus_tag="ACHJWB_005047"
     CDS             complement(454..1008)
                     /locus_tag="ACHJWB_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4316480.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1017..1376)
                     /locus_tag="ACHJWB_005048"
     CDS             complement(1017..1376)
                     /locus_tag="ACHJWB_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4316481.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(1389..>1470)
                     /locus_tag="ACHJWB_005049"
     CDS             complement(1389..>1470)
                     /locus_tag="ACHJWB_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316482.1"
                     /translation="FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          373 a          412 c          286 g          399 t
ORIGIN      
        1 cagccgccca tttaccacaa ataaaaagcc ttcaggactg aatgcgtctg taacaaccgc
       61 actgatagtc tgccagaccc gccataacca gctgggtcag tattaactgg cagcgttcgc
      121 gtgaaaggta agtattctgc gcaatctccc cgactgtcgc cggttcggta acgcttaatt
      181 cattaaacac cactctggcg gtttctgtca tatcctgctg ttttagcatg tctttttccc
      241 ttttccggtt aacgtgacat accaataact cttgtcgaaa aagccagcaa gttgaaagac
      301 cggtattagc aaccaccagc gcgtttaacg ccccgtgccg tttttcagtc ataaaaaaac
      361 ccacaaaaag cgggctcttt caaatgtcca tgtctgctat tcgcctcgcg gtacagcttt
      421 gcgaagctta ccggaattga agcagttttt acgtcaaaaa gcaataactt ttttctctat
      481 accaaaagcc ataaccattg gtttgtacaa aataaattct gccaccttta gccaatgctc
      541 aatgcgtctt tcacaggttc ttaaactcca ttccggatgt gcatcattca gcaattcagc
      601 cattttgcgc ttagtcatcc cccgccccac ataacgctga ctcagaacat tgagcagccc
      661 gggataacct gccagtactt caccaataac cctgtcgatt attaacgcct ctgaatcggt
      721 acaatgtgcc agccagcttt tttgattgcc gttgatcata tcccgcaaaa aagcctcaag
      781 ttcaggtttg tccagacccg cttttttcat cctccggagc gcctcgttaa ttgccgtttt
      841 tgtcagcttt ttagaggtca gtaattggtt gaacatattt cccgtcttac cgtcgccaat
      901 atacgaccaa cgcccccaca tacgcagttt cccctggatc cagacacttt ccagcgtttt
      961 caggcgtaaa tgctcaccgc ttttgcctgt aatttccggg tatatcatat ttatgctcac
     1021 tcactttcaa ttttgtaaat cttcacgccc agccgcccac cagaaacgag ctggccgcgc
     1081 acaatattga tttcatcaaa ctgctcgtcg tctatgagaa gtccggcatg cgtcagcgca
     1141 tccagtggtg ctttcaggat attgtccagg tcacgacggc gcttatccgg tggctctgca
     1201 ataatcttta tcgccagcct tccggacagg tttaatttca gccgctgctg gcgaacaata
     1261 agtgccacat cccggcgata acgctcaccg gcttttgata caaaatatgt gctgccacga
     1321 cgtcgccagt aggtgttcac cgtcggcggg taaggcaaaa caaactctat acgcatcagt
     1381 aacctctttt acccgagcac gccggttgca aaggcgtgat caagaaaacg aaaaattaaa
     1441 tcaacctggg aaccatgctt ttcttcgaac
//
LOCUS       DAZFAH010000415         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFAH010000415 DAZFAH010000000
VERSION     DAZFAH010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.418"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1355
                     /locus_tag="ACHJWB_005050"
     CDS             <1..1355
                     /locus_tag="ACHJWB_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4316483.1"
                     /translation="NSFSVETCRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVII
                     DEASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDS
                     VCMLFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGN
                     HTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRE
                     CDEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHI
                     AALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVAC
                     LLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKT
                     PLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG
                     "
BASE COUNT          401 a          273 c          361 g          428 t
ORIGIN      
        1 gaaattcatt ctcggtagag acatgcaggg atgaatttaa ccgttttata aagcgttttc
       61 cggttattgg tagcagcacg cattctatta ttaactctat cggtaagggg gctcttcttg
      121 attatgtgat tattgatgaa gcctcacagc aggatattgt tccgggcatt ctgggactgg
      181 gatgtgcccg taatgtaatc gttgtagggg atcgaaagca acttccacat gttcctgtat
      241 tactcccgaa cagcccatct ccaccggcag aatactataa ttgtgagaaa tatagtctac
      301 ttgattcggt ttgtatgttg ttcaggaata tggtgccagt aactcttctt aaagagcatt
      361 atcgctgtca tcctaaaatc attcaatttt gtaataagca gttttatgat aatgctctga
      421 taccgctaac gcttgattca ggtgaagcct cattatctct gatcatcacg gcaaaaggaa
      481 accatacccg taatttttca aatctcaggg agcttgaatc tctggaagga cattactggg
      541 atgaggaaag tagcagggga tatatcgccc catataatgc acaggttaat ctggctgaaa
      601 cagtgctgcc tgctgatttt gtgaaatcga cggtgcataa atttcaggga cgggagtgcg
      661 atgaaattgt gttttccact gtgctggata agaagcgcag cagccagcac agtcggaaca
      721 tcgcgtttgt tgataatcct gaactggtga atgtcgctgt ttcaagggcc cgaaataaat
      781 ttacgctggt gactggtaat gatgtatttg aacgccacgc tggacatatt gcggcgctga
      841 ttcgttacat caaatactac gctgatgacg gagagatttt cgagagcccg gtcatatcag
      901 catttgattt gctgtacagt gagtatgaca aatcactgga acgactgaat agtagattga
      961 attcaaatga ttcacacttt aagtcagaac agattgtggc ctgcctgttg cgggatatcc
     1021 tgtcgcagga tagttatcgg agtatgatgt ttcattcaca aatagcgctg aatcagttgg
     1081 tgttgctgga gcggggggat tttacacacc gtgaacagtt attcatgcgt aaccgggcca
     1141 gttgtgactt tgttgtttac tataaggtcg ggaagactcc attaggggtt attgaagtgg
     1201 atggaggtta tcatctgacc agtgttcagg ctgaacgtga tgaacttaaa aacagtatac
     1261 tgaagaaatg cggcctgcca ttgctaagat tacgtactat agacagtgat atagaaggga
     1321 agttgggagc gtttctgtca ggcctgacag gttaaagtct gaataaaaaa tattgcagcg
     1381 ggaggtctca aacctgattt ggtcatgtgc cacacacaat tgtacgaacc ccatggacac
     1441 aggcttcttt gttttggtta cat
//
LOCUS       DAZFAH010000416         1429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFAH010000416 DAZFAH010000000
VERSION     DAZFAH010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.419"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..431)
                     /locus_tag="ACHJWB_005051"
     CDS             complement(<1..431)
                     /locus_tag="ACHJWB_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO4316484.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADSIVQAGETVNGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNG"
     gene            complement(803..>1429)
                     /locus_tag="ACHJWB_005052"
     CDS             complement(803..>1429)
                     /locus_tag="ACHJWB_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4316485.1"
                     /translation="GHSMIITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADD
                     RALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEA
                     VFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQA
                     REQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          316 a          415 c          373 g          325 t
ORIGIN      
        1 ccgttcaggc tctgtccccc gccgtcacga ataaccgtat cactggctgt tcccccctcc
       61 agcacgacct gacgaccatt tgtggtaaca gtggtgtttc cggctatccc gccattctgt
      121 atccattgcc caccggtgtt ttcttcactg tccggcccca gttccagccc ggtactgatg
      181 gtcattccgt tggccgtacc gaggacaatc tggttgtcat gattttccag tgttccgccg
      241 ttcacggttt cacctgcctg tacaatactg tcagcagcca gggccgggac tgacgtgaca
      301 gcagcaagag acagagcaac cgccacaccg gcgccttttc cccgtgagcg ggccagttcg
      361 gaggccacca ccagggtgcc cgtaatgtga ttccatacca gcctgtagct ggtgttcaga
      421 tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac ccacaaatgt
      481 ggataccggc aggtacaaaa cagcatggtc agataaagtg gtttttcccg gacataaatg
      541 cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc cgttctgcat
      601 gatgaagaga ttcagctgcc ttttcgcagg ccattaacca ctataaacga tcgatagtaa
      661 cgatcgatac ctgttttatc gatcgtttta ttctgttaac gacaaggcgg tcacgcaaga
      721 gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg taatgttatc
      781 aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg cgggcaacag
      841 agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca acacagacgc
      901 tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gcctgagcgc
      961 ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg gcggcatggt
     1021 cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg gtgcgggccg
     1081 atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc gttttctccc
     1141 gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat tcatggcagg
     1201 gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc cggtgcagga
     1261 tgtgtcgcca gaaatactca tccacagaca gggcacggtc atcggcttta atcagccaca
     1321 gtaccaggtc aagttcaggc agaatgtcac ggtacagggc ttcatactct gcatccctgt
     1381 cccggctctc gcccaccccg ggcaggtcag tgataatcat gctgtgacc
//
LOCUS       DAZFAH010000417         1400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFAH010000417 DAZFAH010000000
VERSION     DAZFAH010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.420"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..514
                     /locus_tag="ACHJWB_005053"
     CDS             38..514
                     /locus_tag="ACHJWB_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4316486.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            511..>1400
                     /locus_tag="ACHJWB_005054"
     CDS             511..>1400
                     /locus_tag="ACHJWB_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4316487.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAP"
BASE COUNT          316 a          348 c          413 g          323 t
ORIGIN      
        1 tcactggtgt gtgtttacat aattttcagg gggagttatg gatcgtgaat taaaaaatct
       61 gcatctgaat atttcccaac tggccgcatt atccggtgct catcgacaga ctgttgcggc
      121 tcgggtaaaa aacataagcc cagccggtgg tcatgagagc aatctcaaac tgtaccgact
      181 gacagatatc cttgccgagc tgatgaaagc tcctctgcct gtagataacg aggaaatgga
      241 tcctcatgcg cgtaaagcat ggtaccagtc agaacgtgac cgactgaaat ttgagcagga
      301 aactggtcag cttgtgccag tcagtgatgt caggcggtcc ttttctgtcg tggtgaaagc
      361 gatagttcag gtactggaaa cctggcctga ccggctggag agggacaggg ggtggaccgc
      421 atcacaactg aatgaagtac agattgtggt tgatgagatc cgcgacacac tggaaaaggc
      481 agtcattgac tgttgtgatg aggccgatat gtgaatcagg tgaacgagag ccatagccgc
      541 gcatccgata tctggcgcga agtggcctcg ctgtttcgcc cacccagccg gttaccagta
      601 gcggaagcca tcaggcgtta tatgcgggtt ccacggggag ccaatacttc cggtccgtgg
      661 gagtcatcgc tgacgcccta tatgattgac cccattaata cattatcagc ccgtgaatat
      721 gacgcggtgg tgtttgtggg acctgcgcga accgggaaaa ccgaagggct gattgatggc
      781 tggattgtgt acggcatcat ctgtgatccg gcggatatgc tggtggtgca gatgactgag
      841 acgaaggcgc gtgagcattc cagaacgcgt ctttccagga cgtttcgcca cagtccggag
      901 gtcagcaagc gcctcagtcc ttcccgtaat gacaacaacg tccacgataa aatgtttctt
      961 gacggctcct tcctgaagat tggctggccg tcgatcaccg tcttttcctc ttcggattac
     1021 cgtcgtgtgg cgctgacgga ttatgaccgt ttccctgaaa acgtggacgg ggaaggggat
     1081 gccttcacgc tggcctcaaa gcgtaccacc acctttatgt cctcggggat gaccctggtc
     1141 gagagttcac cggggcggga tatcaccgat accaaatggc gttgtggtgg cgcacatgag
     1201 gcaccgccaa caacggggat cctgtcactg tataaccggg gagaccgccg ccggtggtac
     1261 tggccgtgtc cgcactgcgg ggaatatttt cagccggtga tggataacat gaccggatac
     1321 cggaataacc cggattttgt ggctgccggt caggctgccc gtctgatgtg tccgcattgt
     1381 cgcgggctga ttgcccctga
//
LOCUS       DAZFAH010000418         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFAH010000418 DAZFAH010000000
VERSION     DAZFAH010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.421"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..268
                     /locus_tag="ACHJWB_005055"
     CDS             <1..268
                     /locus_tag="ACHJWB_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316488.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..1170
                     /locus_tag="ACHJWB_005056"
     CDS             265..1170
                     /locus_tag="ACHJWB_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4316489.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            1167..>1372
                     /locus_tag="ACHJWB_005057"
     CDS             1167..>1372
                     /locus_tag="ACHJWB_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4316490.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          328 a          333 c          416 g          295 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcgtttgg tgatgtgata
      841 cgtcagggcg aaacaggcca ggttaaccag ctactggata ttctcagaca taaagcatta
      901 acgcagatgg cacaggagag tggcggtagt gcaaccgtgc gtctgaacac tctggactgg
      961 cttggtggtc agggccggga gcaggcagat aacgagtggc atgacgcgat taactggctg
     1021 ggagactggt gcagtgaaga gcagcatccg gtgatctggt caaccacgca ggctgcagaa
     1081 cacttaccgg ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga aagtatggtc
     1141 gatgaaatat ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg ctggtgctgt
     1201 ccggtggcgg ggcggtgggc gcttatcagg cgggagtggt taaggcactg gcagagtgtg
     1261 gtacacagat cagcatggtt tcagggacca gcattggcgc attcaatggt gccattatcg
     1321 cggcctctcc cgatctgtca gaagctgccg tacgcctgga ggcgctctgg ga
//
LOCUS       DAZFAH010000419         1352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFAH010000419 DAZFAH010000000
VERSION     DAZFAH010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.422"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(55..450)
                     /locus_tag="ACHJWB_005058"
     CDS             complement(55..450)
                     /locus_tag="ACHJWB_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4316491.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(447..1025)
                     /locus_tag="ACHJWB_005059"
     CDS             complement(447..1025)
                     /locus_tag="ACHJWB_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316492.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1037..>1352)
                     /locus_tag="ACHJWB_005060"
     CDS             complement(1037..>1352)
                     /locus_tag="ACHJWB_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4316493.1"
                     /translation="RADETIRGYMGTSATMTSGEQSGAVIRGVFDDPENISYAGQGVR
                     VEGSSPSLFVRTDDVWQLRRGDTLTIGEENFWIDRVSPDDGGSCHLWLGRGVPPAVNR
                     RR"
BASE COUNT          290 a          425 c          326 g          311 t
ORIGIN      
        1 gtggtaccgg caccttttac cggctcaagg ggatttggtg ttgccatatc gtcctcacat
       61 ctcgtatgta atggaataag tcagatctgc agaactccat aacgccatat cgtcatcacg
      121 acgatactca tagccctgcg taaccatcgt ggtaatcagt cctgccagtg ccgggatatc
      181 gctcatcacc ggataaatcc gggactccat ccacgcatcc agctctgaat ccggtacctg
      241 agcaggcagg aaaacttcaa tatgcagctc cgcctgccag gtatcgctgt ccagctcttc
      301 gcccgtgtat tcagcgccgg tgagataaac ggcaactgcc ggaaaatcct cctcatcaaa
      361 aacagcgggg cgaccatcaa aaagcgtcgc cccggtgtca tgcttctcca gtgcatccag
      421 tacggctgca cggagttcag tatgtttcat cgctttatta ccattctcag ttgatgctgc
      481 agcgcatagc ccagctcttt cggaagacgt tcacgccgta tccgttcaat attctgttta
      541 aacgccgtgg taagcggcac cgccatcggg attttcacca catcaatggg gtaacggttt
      601 ttcccggcca cacgctgcat gacatgccac cgcccatttt tcagttgctg aataaacgcg
      661 ccgggaatac gacggttacc caccacaagc acgctgccgc cacctttcag ggatgaacgc
      721 tgcccctttt tacgacgcct gcgtcgggac aggacaaccc gcgcattccc cagcttgatt
      781 acgggcaaat ccccccggtt aaccctgatt ctggcctgcg gatttttaac cgtggccctt
      841 ttcagcctgg ccctttcctt taccagtttc cggcgtacct ttgtctcacg ggcaacctgt
      901 gacgcagact gcgatatcgc ggatgaagca acgcggttaa tggccattgc ggcggcacca
      961 ggcaccgccg ttttgctgat acggctgagg ttttcaatgg cctgctcaag accttttatg
     1021 gccatacatc cccctttcag cggcgacggt taacggcagg cggtacgccc cgcccaagcc
     1081 agagatgaca gcttccacca tcatccggcg aaacccggtc tatccagaag ttttcctcac
     1141 cgatggtcag cgtgtctcca cgccgcagct gccacacatc atcagtccgg acaaacaggg
     1201 acgggctgga gccttcaacg cgcacgccct gtccggcata gctgatattt tcagggtcat
     1261 caaaaacacc acgtattact gcgccggact gctcaccgga tgtcatggtg gctgacgttc
     1321 ccatgtaccc gcgtatcgtt tcatcggcgc gg
//
LOCUS       DAZFAH010000420         1339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFAH010000420 DAZFAH010000000
VERSION     DAZFAH010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.423"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            493..>1339
                     /locus_tag="ACHJWB_005061"
     CDS             493..>1339
                     /locus_tag="ACHJWB_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316494.1"
                     /translation="MVTKKVKTNHPYYRVLSGTSMPFPHIHEAVSEQLLLLIFKARRL
                     YLDPEYRPWFDEQHRILSALRANTKLQNILEQNREDYSIGETDNPLSKELYAAFQVMC
                     HLYNGFDNRLVSLKTWGDMNREPTAKFVGQNSGGWKTPEMEEFVRNDLFNILTDCKAH
                     CSPLPALVRQGYCHQDLSVAHSCTKELLEQLNRYHQIDESAILARTLGLAINNCYHSK
                     TEIPEWLSPFENEYKSPWNCEKPDTNDVSKLRKQLLTIGRTEFVDKMFSLANKNATKI
                     IYDGYV"
BASE COUNT          468 a          264 c          257 g          350 t
ORIGIN      
        1 agacaaaaac aaaaaaaatc ttgttatgat ctatgatatc aagaggaggg gtagtgacca
       61 aaaattttcc ccagaaaagc ccccacaaca ttagtttgaa aacgtgacga agttcacaaa
      121 attatcttct ggcgataata gaacttgtaa gagggagttg atttttgcca acttgctaaa
      181 gctaaagtgc agacaaaaga caataattga atgaaaacga tacatatttt cagatgaaag
      241 tgacgtgact gtagagggct ataaccgtaa aatcatttgc gtcctgagca atatgcaaca
      301 aaagggaatg ctcgtaaaaa aatcaggaat aaacgctaag gatatcgtat tagattcagc
      361 atcgtataat gccactgcat aactgattcc catttcgtag aggctgttaa ctctgtatca
      421 aagtcctgac aaaggactgt atcaagtttc tctactgaaa cagctatgca atagacggaa
      481 gaatttaaca ttatggtaac taaaaaggta aaaacaaacc acccgtacta tcgtgtttta
      541 tctggcacct ccatgccgtt tccccacata catgaagctg tttccgaaca gttgcttcta
      601 ctcattttca aagcacgcag attgtatctg gatccagaat atcgtccctg gtttgatgaa
      661 caacatcgga ttctctcggc attacgcgct aacacaaaac tacaaaatat tttagagcaa
      721 aatagagagg actactccat aggtgagacc gataatccac tatctaaaga gctgtatgct
      781 gcttttcagg ttatgtgcca tttatacaat ggttttgata acagactcgt cagcctgaaa
      841 acctggggag atatgaacag ggaaccaaca gcaaaatttg tcggccaaaa tagtggtggc
      901 tggaaaacac ctgagatgga agaattcgtc agaaacgatt tgttcaatat tctgacagat
      961 tgcaaagctc attgctcacc acttcccgct ctggttcgac aaggatattg ccatcaggat
     1021 ctttctgttg cgcactcatg tactaaagaa ttactggaac aactgaaccg ataccatcaa
     1081 attgatgaat cagcgatact tgccagaacc cttggtttgg ctattaacaa ctgctatcac
     1141 tcaaaaacag agattcctga atggctttct ccttttgaaa atgaatacaa aagtccctgg
     1201 aattgtgaga aaccagatac taacgatgtg agtaaactta gaaaacaatt gctaacaatc
     1261 gggcgtacag aattcgtgga caaaatgttt tcgctagcca acaaaaatgc aacaaagata
     1321 atatatgatg gatacgtta
//
LOCUS       DAZFAH010000421         1337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFAH010000421 DAZFAH010000000
VERSION     DAZFAH010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.424"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..338)
                     /locus_tag="ACHJWB_005062"
     CDS             complement(<1..338)
                     /locus_tag="ACHJWB_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO4316495.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKR"
     gene            complement(559..1290)
                     /gene="bluR"
                     /locus_tag="ACHJWB_005063"
     CDS             complement(559..1290)
                     /gene="bluR"
                     /locus_tag="ACHJWB_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO4316496.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYTAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          345 a          309 c          299 g          384 t
ORIGIN      
        1 ctgcgtttgt tatgattaac atcagtaacc caacggaaaa cgcccgcgcc tgctagtgtt
       61 gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc ctcttttatt
      121 ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct taataacaaa
      181 accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      241 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      301 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      361 ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      421 ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg agcattttgc
      481 aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt ctgtctaagg
      541 ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt gctcacccca
      601 atgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
      661 ttgcgtcggg aacagttccg ggcgtggcga ttcaatggga tcagcaagaa cagaaatgtg
      721 ccatcctgac aatgataaac gccaggcttc cagccacaat cgtgctctct cttcaacatt
      781 ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa gcgaggctgc
      841 tgtgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg tgttgtgatc
      901 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
      961 ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca tacgaagaat
     1021 tgacatcatc tcttcttgca agcggctcca gtcatcttcc gtatcctggc tggtggtttc
     1081 cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct tgatctcttc
     1141 gatacgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gctgtggttt
     1201 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1261 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcata aatctctgcc
     1321 gcctgtacac gacccaa
//
LOCUS       DAZFAH010000422         1322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFAH010000422 DAZFAH010000000
VERSION     DAZFAH010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.425"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..342
                     /locus_tag="ACHJWB_005064"
     CDS             79..342
                     /locus_tag="ACHJWB_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4316497.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            644..784
                     /locus_tag="ACHJWB_005065"
     CDS             644..784
                     /locus_tag="ACHJWB_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4316498.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          353 a          284 c          328 g          357 t
ORIGIN      
        1 ataagaacag acagttgaat ctgaccgtct gaatatgtac ctttatgcgg aattttcact
       61 taaaggggga aaactgttgt gaaaccgaat atccatccgg aataccgtac agtagtattt
      121 cacgatacca gtattgatga gtacttcagg atcggttcga cgatcaaaac tgaccgggta
      181 attgaactgg atggtgtaac ttacccatat gttacaattg acgtttcttc taagtcgcat
      241 ccgttttata caggtaaact gagaactgtc gcttcagaag gtaatgttgc acgctttacc
      301 cagcgttttg ggcgttttgt tgatgcgaaa aaaggtggct gattttgccg ccagggtgcg
      361 tttgacaatt taaaaacgca ccagacagag agatcctgca cctgaatata ccgttagccc
      421 gtcccaccgg ggcgtgtgca gatgatctcg tcaccggaag attgcggacc tctctgaaaa
      481 ccagtacatt acgcgcgggt gcctttcggc tgacggctgg aaggattacc tgaaggccgg
      541 atgtggaaag gccccgagtc aacttaaacg ttaacccgag gccctgacca tctaccttca
      601 gcaagtggaa ggttagcgcc tctctgtaaa aggagcaagt gctatgccgc aaaaaacgat
      661 cattgtgggc atgttatgcc tgaccatgtt gctgaccgtc tgggtgttac acgcatcacc
      721 gtgtgaattc agggtaagct tcatgtggtc ggaaattgcg gcgttcttgc agtgtaaacc
      781 gtaagaaacc gcagcgggga caacagtccc cgctatccgg ttgccaaagg ttcggtcgaa
      841 agtcgcaccc ttgcacacaa atcacaaaga aaaaacaaaa gtccgcagtg taaaaactgt
      901 gggctttttt attcgcatcc cgaaagtgtg gtaacagcgt gggggcacca aaaaaagccc
      961 cataaaatgg ggctgaagat tctgtaagga atttcacagc aatgagattc ctgctttctc
     1021 caaggatata cgtgtcttca ggctgaatca atgggggcgt ggctctgaat ccacctgcga
     1081 ttaaaacttg cacaatgccg ggagttgagt tagaaaagta cagggcgcga tatccggctt
     1141 attttgttgc tgtgttttgc ctcgtgctgg atgtcgcgtc caccgttttt ctgatgattc
     1201 atatgaaatg gtactttatt gatttactgc cattctgatt tgccttttta tgttcattat
     1261 tgcggttgtc acttccagtt tctggtgatg ttttgataaa agtgaaagaa taaaacgata
     1321 at
//
LOCUS       DAZFAH010000423         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFAH010000423 DAZFAH010000000
VERSION     DAZFAH010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.426"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..236)
                     /locus_tag="ACHJWB_005066"
     CDS             complement(<1..236)
                     /locus_tag="ACHJWB_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316499.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYA"
     gene            complement(233..>1309)
                     /locus_tag="ACHJWB_005067"
     CDS             complement(233..>1309)
                     /locus_tag="ACHJWB_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316500.1"
                     /translation="SRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYK
                     RQLADLGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVA
                     TQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAMGGAFG
                     GGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNL
                     YRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDM
                     ARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          248 a          468 c          303 g          290 t
ORIGIN      
        1 tatgcatagg gtggcttcca caaaaatgcc ttccagcctc cgtgctctgc caggaacgct
       61 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
      121 gcattcagcc ctgccgccat acgctgtgag tacccatcac caaaacgcac ttcacgcacc
      181 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaaggt tttcatcgtc
      241 cgctccctga taacataccg ccatcacgca actgcagccg gagttcatcc tgcgccccct
      301 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttgataccgt
      361 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      421 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      481 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcggt
      541 gaactatccc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat
      601 ggaagttcgc cgccgcagcc tcaatggccg tccccgagga agcagatgca ccaccaccga
      661 aagcaccgcc catggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      721 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      781 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gccacgcttt
      841 taacctgcga aaaactgtcc gtcgcacttt ctgcccactc gccccagccg gacttcagac
      901 cggccatcca gcttccacga agctgctcct ccgcagacca ggtgttcttc agtgccgatg
      961 tggccttcgc cagcgcagcc ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag
     1021 actcccgctg cgcctgacgg tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct
     1081 tcgcgctctg ctgctcttca aaccgcaccg cctgctgtgc cagctcattc aggcgtttct
     1141 ggtgttcaac cttgtcgccc aggtcagcca gctggcgttt gtactccagc gtctctttct
     1201 catgggccag cagggatttt tcctgccagg ataactgccg tttcgtggca gcctctttca
     1261 ggaccgcata ctgactttcc gccttccata aatcacggcg ctgccgact
//
LOCUS       DAZFAH010000424         1308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFAH010000424 DAZFAH010000000
VERSION     DAZFAH010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.427"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..472
                     /locus_tag="ACHJWB_005068"
     CDS             <1..472
                     /locus_tag="ACHJWB_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4316501.1"
                     /translation="WWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGID
                     MPDFEREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVANHAAIYCG
                     NGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            469..1050
                     /locus_tag="ACHJWB_005069"
     CDS             469..1050
                     /locus_tag="ACHJWB_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316502.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
BASE COUNT          287 a          323 c          419 g          279 t
ORIGIN      
        1 ctggtggctg gtctgccggg gggacattca taaattccgc tgtgtgccac atctgacagg
       61 acggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg cataccatct
      121 ggcgggaatt gatatgccgg attttgagcg tgaggatgac tggtggcgca acggtcagaa
      181 cctttacctg gacaatatgg cggtcaccgg cttttaccgg gtgcccctgt cctctgcaca
      241 ggcgggcgat atcctgctgt gctgctttgg cgcatcggtg gccaatcatg ccgccattta
      301 ctgtggcaac ggtgagctgc ttcaccatct gcctgaacaa ctgagtaaac gggagaggta
      361 ttccgaaaaa tggcaacgac gaacgcattc tgtctggcgt caccgccact ggcacgcatc
      421 tgccttcacg gggatttaca acgatttggc cgccgcctca gcctgtatgt gaacacggca
      481 gcggaagcca tccgggcgct gtcgttacag gtgcccggat tccgccgtca gatgaacgaa
      541 ggctggtacc agatacgtat tgccggtgat gacacggcac cggaggcggt gtacgcccgt
      601 cttcacgaac agctgggtga gggggcggtc atccatattg tgccgcgact ggccggggcc
      661 ggaaagggtg gactgcagat tgtgctgggg gcggcagcca tcgtgggctc tttcttcact
      721 gccggggcat caatggcgtt atggggtaca gccctgagtg ccggtggttt ttctgccacc
      781 acgatgctgt tttcactggg ggccagcatg attctgggcg gtgtggccca gatgctggcc
      841 ccgaaggcaa aaacaccgga ttaccgcgca acggataacg gcagacagaa cacgtatttt
      901 tcgtcactgg ataacatgat tgctcagggg aacccgatgc cggtgcctta tggtgaaatg
      961 ctggttggtt cacgacggat atcccaggac atcagcaccc gtgatgaggg cggagacggg
     1021 aaggtggtgg ttatcgggcg gcaggcataa aagcgaaaaa atcccgcagt gaccgaaggc
     1081 tgcgggaaca gaaaatgaag attaaccaca gggggttttg tttttattgg cccgaaaaaa
     1141 ctgtaacgcc cgggaatgat atctgccacg ggggcgtaca ggaaatgtga agaaattcag
     1201 aaattttatt ccgtcatgac acaggcaccc tccggggtgc ctgtcgtttt tggggcataa
     1261 acagattcag acatcagaca ggagaggggg accgagtggg taaaggtg
//
LOCUS       DAZFAH010000425         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFAH010000425 DAZFAH010000000
VERSION     DAZFAH010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.428"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..444
                     /gene="stpA"
                     /locus_tag="ACHJWB_005070"
     CDS             40..444
                     /gene="stpA"
                     /locus_tag="ACHJWB_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4316503.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(491..1015)
                     /gene="ygaP"
                     /locus_tag="ACHJWB_005071"
     CDS             complement(491..1015)
                     /gene="ygaP"
                     /locus_tag="ACHJWB_005071"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4316504.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1025..>1307)
                     /locus_tag="ACHJWB_005072"
     CDS             complement(1025..>1307)
                     /locus_tag="ACHJWB_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4316505.1"
                     /translation="LQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGL
                     SASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          344 a          316 c          315 g          332 t
ORIGIN      
        1 aatacttttt tgttttggcg ttaaaaggtt ttctttatta tgtccgtaat gttacaaagt
       61 ttaaataaca ttcgcaccct ccgtgcgatg gctcgcgaat tctccattga cgttcttgaa
      121 gaaatgctcg aaaaattcag ggttgtcact aaagaaagac gtgaagaaga agaacagcag
      181 cagcgtgaac tggctgagcg tcaggaaaaa attagcacct ggctggagct gatgaaggct
      241 gacggaatta acccggaaga gttattgggt aatagctctg ctgctgcacc acgcgctggt
      301 aaaaaacgcc agccgcgtcc ggcgaaatat aaattcaccg atgttaacgg tgaaactaaa
      361 acctggaccg gtcagggccg tacaccgaag ccaattgctc aggcgctggc agaaggtaaa
      421 tctctcgacg atttcctgat ctaattactg aggccggatg tcgctgctag ggcgcgtccg
      481 gcgtcgcttc tcaagctcgt tggttccacg gcatcttatc taacaacctt gccattccac
      541 aaaaaccgct gattcctgca aacagtaacc cggcaccaac aaagccactt aataagaaga
      601 aaccgctatt tacggtataa cccagtacaa cgccgattaa tattaaacca cccgcagcga
      661 tctgcacctg gcgcattaac ggcaagggtt gagatttatt taccgctact ggcaatccag
      721 ctcttttcca gccatcaatc ccatcttcga gtaaaaatat ttctgcgggg gcggcaatcg
      781 ctgctaattt atcggcgtta ttactggtgc gtttacctgc ctggcagtgg aatataattt
      841 gctcgcgacg taatttaggt ggaagacctg actgttccag cacggataat ggagccagat
      901 ctgcttcagg aatatgttca cgaagatatt catcagcatc acgaatatcg attaactttg
      961 cgccgcgtgc gattaattct tgtgcatcat gcggcgaaat ggttgtcaaa gccatagtga
     1021 ctccttacgg acaatagaca tttttcaggg tggcgataat ggcatttacc gcctcatttt
     1081 taatggaata tagaatgcgt tgggcatccc gttggctgtc gataagccct tcgtcccgca
     1141 tacgagcgag atgctgtgat gtcgcagagg cactcagtcc ggtaatgcgc gtcagctctc
     1201 ccgcgctggt gccgggggaa ccgctaagca tgcacagaat cagcaatcgt ttaggatggc
     1261 tcattgcttt caataaggcc gctgcctgtt cggcactggc ctgtaat
//
LOCUS       DAZFAH010000426         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFAH010000426 DAZFAH010000000
VERSION     DAZFAH010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.429"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..1243
                     /gene="bluF"
                     /locus_tag="ACHJWB_005073"
     CDS             32..1243
                     /gene="bluF"
                     /locus_tag="ACHJWB_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO4316506.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          368 a          281 c          280 g          370 t
ORIGIN      
        1 ctctcgcctc acccattacc ctggattgtt aatgcttacc acccttattt atcgtagcca
       61 tatacgtgac gacgaacctg tcaaaaaaat cgaagaaatg gtttcgatag caaatcgcag
      121 gaacatgcag tctgacgtaa cagggatctt actgtttaat ggttctcatt ttttccagct
      181 tctggaaggt ccggaagaac aggttaaaat gatatatcgg gctatatgcc aggatccacg
      241 gcactataat attgttgagc tgctgtgcga ttacgcgcct gctcgccgtt ttggcaaagc
      301 gggaatggaa ttatttgatt tgcgcctgca cgagcgagat gacgttttac aggccgtatt
      361 cgacaaaggc acatcaaaat ttcagctaac ttatgatgac agagcgctac aattttttcg
      421 tacttttgtc cttgcaaccg aacaatcaac ctatttcgag atccctgccg aagactcctg
      481 gctttttatc gctgacggat ctgataaaga acttgattcc tgtacccttt caccaactat
      541 aaacgaccac tttgcctttc atcctattgt cgatccctta tcgcggcgga taatcgcttt
      601 tgaagccatt gtgcaaaaaa atgaagatag cccatcagcc atagcggttg ggcagcgtaa
      661 agacggggaa atctacacag cggatctcaa aagtaaggcg cttgcattcg cgatggcaca
      721 cgcacttgag ctcggtgata aaatgatttc aatcaatcta ttacctatga cccttgttaa
      781 cgaacctgac gcagtctctt ttttacttga tgaaataaag gccaatgctc tggtgcctga
      841 acaaatcatc gttgaattta ctgaaagtga agttatatct cggtttgatg agtttgcaga
      901 agcgattaaa tcgcttaagg ctgccggtat cagtgtagca attgatcatt ttggcgcagg
      961 ttttgccggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa tcagtcagga
     1021 attgattacc aatgttcata aaagcgggcc acggcaggca attattcaag cgatcataaa
     1081 atgctgtaca tcacttgaaa ttcaagtcag tgctatgggc gtggcaacac cagaagagtg
     1141 gatgtggctt gaatccgcag gaattgagat gtttcaggga gatctgtttg ctaaagctaa
     1201 attgaatggt atcccttcca tagcgtggcc agagaaaaaa taattttcag cacattcttt
     1261 cacatgattt cagtaaatca tcgtgaaaat gaatacgtt
//
LOCUS       DAZFAH010000427         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFAH010000427 DAZFAH010000000
VERSION     DAZFAH010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.430"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..225)
                     /locus_tag="ACHJWB_005074"
     CDS             complement(<1..225)
                     /locus_tag="ACHJWB_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO4316507.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILV"
     gene            complement(253..>1292)
                     /locus_tag="ACHJWB_005075"
     CDS             complement(253..>1292)
                     /locus_tag="ACHJWB_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4316508.1"
                     /translation="MIAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAF
                     LTTKYLGAQGLFVAVIVALITSEIFCRLARNPKITITMPAVVPPAVARSFKVLLPIFF
                     VMVFFSALNYCLTLISPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIH
                     GPNTTSAIRETVFSEANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGL
                     LLAIFIASKRAEYRDLAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAI
                     GYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIA
                     ANKVNNMTTNG"
BASE COUNT          402 a          305 c          275 g          310 t
ORIGIN      
        1 aaccagaatt aaattaactt ttattttgcc acagccttca tcctgactaa ttaatgcggt
       61 ctgaatctta tgtgcctgaa gacaggcaga ttcagaactg gccattagct gagttgcgcc
      121 ctgccagtct ttttgcccgg cacaacgtat ggcttccatc gcgtcagaac gtgcctgccc
      181 cgcgttgacc agtaactcca taacaagttc ttcatctgca aacatcacaa tctccttcct
      241 gggtaaatgg aattatccgt tagttgtcat gttattgact ttgttggcgg caataacaaa
      301 aggtaaatag atcattgtcg ccacacctaa acataaaaaa ccaaccagta aagccagcca
      361 gttccccccg gttccgagga aagcaattaa aggtccgggc gtagtccagg gcacggcata
      421 agcaaccggt ggaataattt ccatcgaaac aaagaagtaa ccaatggcac agttaacaat
      481 gggaaccata ataaacggca ccatcatgat ggggttaagt acaataggaa ggccaaacat
      541 tatcggttca ttgatattga aaataccggg gataaatgac atttttgcca gatcacggta
      601 ttccgcacgc ttagaagcga taaaaatagc caacaataac cccaacgtca tacctgaacc
      661 gccgcagttg gcgaatgcat cattaataga agtccaggta atcggatatg gcgcgcccca
      721 ggtagtgcca tgttgagcgg cccaggagag attctccaga ttagcctcag aaaaaacggt
      781 ttctcgaatt gccgatgtgg tattaggtcc gtggatcccc agcacccaca ggaaattacc
      841 cacagccccc aggataatta ccgcaaagat attcgttccc atatgtttga gcggcgtctg
      901 gattaatgtg taaataagat cgtttagtcc tgccggggat atcagtgtca ggcaataatt
      961 aagtgcggaa aagaacacca tgacaaaaaa tattggcaat aaaactttaa atgaacgcgc
     1021 taccgcaggc ggtacaactg ccggcatcgt tatggtgatt tttgggtttc gagctaagcg
     1081 acaaaatatt tcactggtga tcaatgcaac gataacagca acaaacaacc cctgcgcgcc
     1141 gagatatttg gtcgtcagga atgcttgccc atctatcatc tgatatggcg tataaacaat
     1201 aaaaaatgct cctattgccg ttaatccgca taaaagatca tcttgctcat agctaatcgc
     1261 catattcctg gcgaccagga aagcaatcat aa
//
LOCUS       DAZFAH010000428         1267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFAH010000428 DAZFAH010000000
VERSION     DAZFAH010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.431"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..>1267
                     /gene="espK"
                     /locus_tag="ACHJWB_005076"
     CDS             21..>1267
                     /gene="espK"
                     /locus_tag="ACHJWB_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO4316509.1"
                     /translation="MLPTSQLRPTGTFCSYPAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIM"
BASE COUNT          448 a          283 c          236 g          300 t
ORIGIN      
        1 tctaaaaatg gaatttatca atgcttccta catcgcaatt acgaccgacc gggacattct
       61 gctcctatcc cgctgaaaca tcagcagaca tcaaaagcga aatcacacca attcagatag
      121 aagaagcgcg ggccagtggt cgtttatata tcaaagattg tgatattgag tatctgccac
      181 agttaccaaa cgaaataaca tcagttacaa tcgaaaactg caacaacctg acaaccctta
      241 caggattgcc ggttaataca caaaacctct ccgtcattaa ctgtgaaaaa ttacaaatca
      301 cagacatgcc atcaaccgta aaaaatctac atattgaatt aactgattca ccatttatac
      361 atttcatatc tgaaggcatc gagtgcctga cggtttgcca ctgctatata tctggagtgc
      421 cagagagtgt ccgctacctt gaaataaaag gtagcgccac agacagcata aaaaatgttc
      481 caaacgggtt atcatctctc agcatcaata gctataaccc ggagaatcag gccagaattg
      541 acaacctgat atcaccgtca ctgaagacgc tatcgctgac tggatgtagc aatattatac
      601 tgccggagaa acttccggag agtgtgacat cggtaaccat tcatgcggag cagaaaacca
      661 cgtggaacat cggtgttgaa gggatgcctg atgggttgga tcttgattta caaaatgtac
      721 tactctctcc agatgtagtt aaagcaaaaa acatcacctt tcagggcaac gctctggatg
      781 tggccttaca ctttcgcgag ggagacattg tctatggact atcttcaccc agagaaaaac
      841 ttgtcaacag cattaaacta gttaacgact tttccaaaaa agatattata actcagaata
      901 cgttaacaaa cgcagtatgg gaccccagaa cacctcgcaa atataagcaa gatccactta
      961 tcaaaagagc attaaatgaa cacgaaagag ggataaaatt taaacaacac ttaaagaatc
     1021 acaataatta taatgttacc atggccgacc tttccgtata caatcgcgac aaattatggg
     1081 caaaaacaag caaggccggc ctagagtttc agacattaac acgcaataaa acggttattt
     1141 tttgtgcgga tgagcttgtc aactcactca aactcatagc taacaagtca gagggctatg
     1201 gccagagtat taccgccagc gaattacgat ggatttaccg taataaagac aacaaccaaa
     1261 taatgaa
//
LOCUS       DAZFAH010000429         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFAH010000429 DAZFAH010000000
VERSION     DAZFAH010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.432"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            622..690
                     /locus_tag="ACHJWB_005077"
                     /pseudo
     CDS             622..690
                     /locus_tag="ACHJWB_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            749..1219
                     /locus_tag="ACHJWB_005078"
     CDS             749..1219
                     /locus_tag="ACHJWB_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316510.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          428 a          250 c          276 g          307 t
ORIGIN      
        1 acaaaataaa acaccataag aatcaatacg atatttgaaa accaagagaa tttatcattt
       61 tgctattgcc attaatttat cattccgata aagttcaccc atcagcagga cgcactactc
      121 accagggcgg tgaatataca acgattcgaa tatgaatcta cggcgctgac aaagcgcaat
      181 aaccaaagtg aactttgggg tgaatgcaga agctaacctt ctcggcggag gcgctttgca
      241 atgattacgc gaccggagtt agtcgcccgg ctgtattcac cgccaaagtt catcaggagg
      301 ttccatatga cacgcagaac tcagttcaaa ggcaattctc gttctcgtcg tcgtgagcgt
      361 ttaaaggcaa aggcattagc taacggcgta ctggcccgcg aagaagcaat aagttcagaa
      421 gtattacacc gcccaactct aagtagagcg cagattcagg ctaaaggtac tcacgaaacg
      481 cctgatcgca tagaagacgc taagccaatt aagttcatgg cacaggacgt gatctggcag
      541 caggaagaat acagacgcaa tctggagcga gcggccattg tgtacgcgaa tgagtttgga
      601 cataagcaac cagaaactgg tgtatgtctt ccaaatgtag ctctttacgc ggcaggctac
      661 cggaaatcca aacaactgac agcaagatga cttgtgttgg tcgccagaaa atgaaattag
      721 gcagcaaacc acttatttga ggtgatatat gacaaaatca tggagcgtac cttttcctga
      781 atcagaaact gaacatgatg gaatgcctgt tttctggaga ttccaggcga cagttgaaga
      841 agatgggata aaaatattcg cacttcaata tatagctttt catcagacag agcattatgc
      901 atggttggtt cctgcgcatt ggattgttaa ttttaaacca gcaccaaatc agtggttaca
      961 ggaatggaaa caaaggagaa atagatatgc aattaagaaa gtagcaaaaa atgcagaaag
     1021 atcttttgca ttcccaacga agaaacttgc cattgaaagt ttattgcgcc ggaagaaata
     1081 ccatttaatg agaatcaaac aagatttggc tgttgtatca actcttgttg atggtatgaa
     1141 gaatattgat acatcaacac cagatattga atataacttt ggacacaacc aagaaacaga
     1201 aaattgggta ttttattagt acgaataagc actgtgtatt cattccaacg agtgaataca
     1261 c
//
LOCUS       DAZFAH010000430         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFAH010000430 DAZFAH010000000
VERSION     DAZFAH010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.433"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(127..1251)
                     /locus_tag="ACHJWB_005079"
     CDS             complement(127..1251)
                     /locus_tag="ACHJWB_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO4316511.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          379 a          299 c          223 g          354 t
ORIGIN      
        1 tcagataact ctttctgcaa tattattatg ggagactatc tcatttattt agtgatgtcc
       61 ttcatttatg tgatgaagga cattatatta ctcataacat ttctcgttga aaatttcagc
      121 aatacgctac aattgtttcc cgttgttttt taattttctc atcaaaacct ctgcaatctc
      181 tgcaggactg ggttgttcag ccatttcagc ggctacctga tctgaacaga agcgtagtga
      241 atcatcataa agaaggcgat ttaccaaatc actggtcagt ccatgcttgt ccggaataat
      301 cccacaccca cgcttcgcaa caatttctgc attaacagag cgatctgcac cttcgccaaa
      361 cacaatctgt ggtattccac tatacagcgc tgtcagggta tttcctgcgc caccgtgatg
      421 aataaatcca tcagctccat taaggaatac acccataggt atccagtcaa ccagacgtac
      481 atttgatggt agttttcgta aaccagtacg agcatttatt gcaagttgca aaatgatatc
      541 agcatcaact tcatttgcag aatccataac ccatgaaatc agctccagac catcaaccat
      601 tggttttaca gtgccaaggc tgataagtaa acgtttccga tcagaatccc tgtcccacca
      661 tgtttccttt acagcacctc cgttataagg aatatatctc attgagataa ccggttctcc
      721 agcattttta agaatgctca tacttggggg agcaacatca atccatgcta aatcacatag
      781 tggaccgctg actccatgcc gcctgtaagc attgcttaaa gaacggttta acatctgaat
      841 atgggcagat gtatgcgcga atccaacagc cagcatcact gaaggaattc tatatttagc
      901 agcaaccaat gggcctgccg gatcaagagg gggatagact attaaatctg gcctccattt
      961 tcctgcaaaa tcgatgaggt tatctgccat ttcatcgcta aaaaaagaga aatgaccaat
     1021 aatattactt tttttcctca actcttcctg atggcgatat ccagcctctg aatctaaacc
     1081 tggaactgca tcaaaaacaa ccagtccagc ttctgctgct ttattcgcga atttgccagc
     1141 gctactaatt actacatcat gtccaattac acgaaaggcc tgagccagag aaatcaatgg
     1201 gtataacaaa ccgtacagcg gtgggccaat aaaaagaata cgttttcgca ttaaa
//
LOCUS       DAZFAH010000431         1245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFAH010000431 DAZFAH010000000
VERSION     DAZFAH010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.434"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..102
                     /locus_tag="ACHJWB_005080"
     CDS             <1..102
                     /locus_tag="ACHJWB_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4316512.1"
                     /translation="LLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            180..395
                     /locus_tag="ACHJWB_005081"
     CDS             180..395
                     /locus_tag="ACHJWB_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316513.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGD"
     gene            399..915
                     /locus_tag="ACHJWB_005082"
                     /pseudo
     CDS             399..915
                     /locus_tag="ACHJWB_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            958..>1245
                     /locus_tag="ACHJWB_005083"
     CDS             958..>1245
                     /locus_tag="ACHJWB_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4316514.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIKVP"
BASE COUNT          324 a          265 c          365 g          291 t
ORIGIN      
        1 ctgctgggcg caccggcagc tccggagccg gaaggcatcg cgggtgaggg gagtgcttca
       61 gatagcggtg accccacacc ggacagcgac atgatgatgt aagcatgcgt cagggaccat
      121 cggtgtgtgc cggtggtctt ttttattgtt gtgagcttcc ggattgcggg aggcggggga
      181 tgaaccagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg gtggggacgg
      241 gatactggtt actgcagctg ctggacaaag tctctccgtc ccagtgggtg gcgataggtg
      301 tgctggggag tctgctgttt ggcctgctga cgtatctgac taacctgtat ttcaaaatta
      361 aagaggaccg tcgcaaggct gcccggggag attaggtgat gaaccatgaa gaaatgaatc
      421 agcgcttcag tcgtctggaa aatgaaattg ctgaactgaa taaaaaactg tcgacgctga
      481 tgccttctga agatgaaaaa aaacgccgcg atgagcagaa atctctcaga gtgtttcagt
      541 attcataatg ataatttcag tgagctggaa tgggagtgta accggccatc ctttgttgta
      601 tccggtgatg ctgggaaaat aaccatctca gaaaatggaa aagtaacacc gccatcgcac
      661 cagcatagtg aggagctcat tgaatttgcc attgattacc tgaagaacaa taaaaaacag
      721 gggctgatga agtgcattgg tcgttgcatg ggatatctgc agatagctgc tgagattgaa
      781 gcgctggcca gtggtgcgga caaggatgca gttgtgcggg aggctcttct tcgtgagttt
      841 gacaacccgc cctttaaaaa agtgccggct tactggtttc atccaggact gacttatctt
      901 aaaggacgta tataagctgg ctcgttatct gttgtcgata aatcctgata aatatccatg
      961 aacaccaaaa tcaaatacgg cctgtcggct gccgttctgg cgctgattgc cgctggtgcg
     1021 cctgcgcctg acattctcga ccagtttctg gatgaaaagg aaggtaacca caccacggca
     1081 taccgtgatg gtgcgggtat ctggaccatc tgccgaggtg ccatcatggt ggatggtaag
     1141 cctgtgattc ctggcatgaa gctgtcgaag gaaaaatgcg accaggttaa cgccattgag
     1201 cgtgataagg cgctggcatg ggtggagaaa aacatcaaag tgccg
//
LOCUS       DAZFAH010000432         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFAH010000432 DAZFAH010000000
VERSION     DAZFAH010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.435"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>1233
                     /gene="nleA"
                     /locus_tag="ACHJWB_005084"
     CDS             33..>1233
                     /gene="nleA"
                     /locus_tag="ACHJWB_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO4316515.1"
                     /translation="MNIQPTIQSGITTQHNQHHHTEQTPPTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTVLLSLIKDKGLLSGLDAYIAPHLEEGSAGKKALDTFGLFNV
                     TQMALEIPSSTVPGISGKYGVQMNIVKPDIHPTTGNYVLQLFPAHDEIGINYRDLQGP
                     LKNVLSSDAVPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWV
                     IRPAEADLKYGTSPFQATLGLEFGAHFKWDLDNSRTKYAILTNAAANAVGAAGGFAVS
                     RLPGVDPMLAPHIGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAEFGVA
                     RFTDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESN
                     DSPHHNPS"
BASE COUNT          383 a          270 c          278 g          302 t
ORIGIN      
        1 aatggatata aattacgtca taaggattta tcatgaacat tcaaccgacc atacaatccg
       61 gaattaccac acaacacaat caacatcatc acacagaaca aacgccccca acacaaatac
      121 cgcaatccga attacctcta ggatgccaag ctggatttgt tgttaatatt ccagatgata
      181 tacagcaaca tgcaccggaa tgcggtgaaa caacagttct actgagcttg ataaaagata
      241 aaggtctgct ctcagggtta gacgcatata ttgctcctca ccttgaagaa ggctcagcag
      301 gaaaaaaagc attggatacg tttggtttat tcaatgttac tcagatggca ttagaaatac
      361 ccagcagcac cgttcctggt atctctggta aatatggtgt tcagatgaac attgtaaaac
      421 cagatataca tcccacaacc gggaactatg tcttacagtt atttcctgca catgatgaaa
      481 taggaattaa ctacagagac cttcaaggac cactaaaaaa tgtattgtca tcggatgcag
      541 tgccttggtt tggattaacg gctcaggttg ttcgtaacca tggtgtagag cttccaattg
      601 taaaaaccga aaatgggtgg aagcttgtcg gggaaacccc tcttactcct gatggaccta
      661 aagccaatta tacggaagag tgggttatca gaccagcgga agctgattta aaatatggaa
      721 catccccatt ccaggcgact ctgggactgg agtttggcgc acattttaag tgggatttag
      781 ataattctcg aaccaaatat gccattctta ccaatgcggc agcgaatgcg gttggcgcag
      841 cagggggatt tgcggtatcc agactccccg gagtagaccc aatgttagcc cctcatattg
      901 gttcaatggc cggacaggct ttggggcatg ctgtacaatg ctacactcca ggattaaagc
      961 cagacactat tttatggtgg gcaggtacga ctctggggct ggcagattta aacaaggccg
     1021 agtttggagt agctagattc actgactatc ctcgtatatg gtggcatgca agagaaggtg
     1081 ccatcttccc taataaagaa gatattgccc gtgcaacagg ggctgatata cgagctatgg
     1141 aagaaggtat acccgttgga cagcgacatc caaatccaga ggatgtggta atcgatatcg
     1201 aaagcaatga ctcaccacat cataacccat caa
//
LOCUS       DAZFAH010000433         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFAH010000433 DAZFAH010000000
VERSION     DAZFAH010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.436"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1145
                     /locus_tag="ACHJWB_005085"
     CDS             <1..1145
                     /locus_tag="ACHJWB_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO4316516.1"
                     /translation="HALFLMVIFLLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQV
                     IQTLDPGILKTLEVREGDIVEKGQVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAE
                     AYSESLTFPDDVPADLRERESTVYRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVRE
                     GAMSEVELLRMQRQSAELQLQMDEKQNKYLTEAGAELVKTEAELAQAKENMAGRADPV
                     ERSRIRAPLRGIVKNIRVNTLGGVVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTG
                     MPALVKLTAYDYAIYGGLDGVVILVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNY
                     LTDRNGKILPVIPGMIASVDIKTGQKSVFQYLIKPITRMKQALQER"
BASE COUNT          352 a          235 c          332 g          284 t
ORIGIN      
        1 atcatgccct tttcctgatg gtgatttttc tgctggtact ggtagtgacg tttgttatct
       61 gggcatggaa cagtcctctg gatgaagtga cccgtggtca ggggagtatt attcccggca
      121 gcagggagca ggttatacag acccttgacc cgggtatttt gaaaacactt gaggtcaggg
      181 aaggcgatat tgttgagaaa ggtcaggtac tgctgacact ggatgatacc cgaagctctg
      241 ccatgctgag ggaaagtgaa gcccgggtga ataacctgga agcagtcagg gcaagactcc
      301 gtgcagaagc ttattccgaa tcactgacat ttccggatga cgtgcctgca gatttgaggg
      361 agagggaaag tacagtttat cggctacgaa agactgaact ggcacaaagc attgccggcc
      421 ttaagcagag taaggctctg ctggataagg aaatagcaat gacccggcca attgtgcgtg
      481 aaggggcgat gtcggaagtc gaactgttac gtatgcagcg tcagtcagcg gagctgcagt
      541 tacagatgga tgagaaacag aataaatacc tgacggaagc tggagctgaa ctggtgaaga
      601 cagaagcaga gcttgcacag gcaaaagaga atatggctgg cagagccgac ccggttgagc
      661 gttcacgtat cagggctccc ctgagaggca tcgtaaaaaa tattcgggta aatactttgg
      721 gaggcgtggt ttctgcagga caggatataa tggaaattat tccgctggaa gaccagttgc
      781 tgattgaagc ctatataaat ccacgggatg ttgcctatgt cagaacaggg atgccggcac
      841 ttgtaaaact gacggcttat gactatgcca tttatggtgg tcttgacgga gtggtgatac
      901 tggtaagtcc ggatacttta cgtgaccaga aacggccggg agatttaaaa ctggatccaa
      961 atgaagcata ttatcgggta ctcgttacta ccagtaataa ttatctgaca gacaggaacg
     1021 gaaaaatatt accagttatt cctggcatga tagccagcgt ggatataaaa acaggtcaga
     1081 aaagtgtatt tcagtacctg attaaaccaa tcaccagaat gaaacaagct cttcaggaac
     1141 gctgatttat catattatta gcctcatggc tccgtaatac agacactaaa caggaagtct
     1201 gta
//
LOCUS       DAZFAH010000434         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFAH010000434 DAZFAH010000000
VERSION     DAZFAH010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.437"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..791
                     /locus_tag="ACHJWB_005086"
     CDS             66..791
                     /locus_tag="ACHJWB_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4316517.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQDGKFRYCIPGVDLPAEQKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTETKADDLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKNWDIVRQIVDSSGRIVSEK"
     gene            807..>1196
                     /locus_tag="ACHJWB_005087"
     CDS             807..>1196
                     /locus_tag="ACHJWB_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4316518.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLEGATGAGWHSVK
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAV"
BASE COUNT          333 a          253 c          356 g          254 t
ORIGIN      
        1 atagctggcg tccaaatgtc agcaatcaga gggttgtgaa gtaatttttg ttggaggacg
       61 ttttaatgga aactgtattt gacgcactga aagcactgaa aaaagcctct tcacaggtag
      121 tggcatcgcg ccttggaatc agccgcgaag atgctgtcaa cgaactgtgg aaactgaagc
      181 gccgtggtga agcggataac aaggggtcga tgtggtggct gactcagact ggtgaaagtg
      241 aaccagtgtc accggtaccg aaagtgacag cgcaaatgct gactgaggcg attgaacatc
      301 atggcccaca aacggcggat gagctggcat tgatgttcgg aattacctcc cgccgtgcga
      361 attcatcgct ggcaatggca atcagcaaag ggcgtctgat tcgcgtgaat caggatggta
      421 aatttcgtta ctgcataccg ggcgttgatt taccggcaga gcagaaagct gcatccgtag
      481 ctgaaacgga gggtaaagcc cttcctcagc cagcaggtgt tgcgttacca gtccaggaaa
      541 cagctgcaca ggaagaaatt aaaacagaag cggtggagga cattgtgaag ttgcagcaat
      601 cgttcactga aacgaaagca gatgacctga ttctaccatc gctgcatgtg gctaaccgcg
      661 agctgcgccg agcaaaaggt caggttcaga agtgggagcg agtctgcgcc gcgctgcggg
      721 agttgaacaa gaactgggat attgttcggc agattgtcga ttcatccggt cgtattgtgt
      781 cggaaaagtg atagccggag gcgcttatgg cgaaaccttt tacacacgaa cagcgtgaag
      841 aactgaaggc ccgaattatc gggctggtac gcaaaaatga acgcatgacg atatcacaac
      901 tggagggagc gacgggagca ggctggcatt cagtcaaacg ttgccttgtg gatgtactgg
      961 cttgcggcga tttatacatg cccggtaaat acggtgtttt tacatcagaa caggtgtatc
     1021 gcgtatggcg taaggcagcg gagaaaacaa ccgaccagac attgattcga aagttaccag
     1081 acggagaaat acgccgctac gacagacaac agaacataat ctgtggcgag tgccggaaga
     1141 gtgaagttat gctgcgtgta ctggcgttct atcagggcaa ttttcaggag gcggta
//
LOCUS       DAZFAH010000435         1188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFAH010000435 DAZFAH010000000
VERSION     DAZFAH010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.438"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..161)
                     /locus_tag="ACHJWB_005088"
     CDS             complement(<1..161)
                     /locus_tag="ACHJWB_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4316519.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQ"
     gene            complement(173..946)
                     /locus_tag="ACHJWB_005089"
                     /pseudo
     CDS             complement(173..946)
                     /locus_tag="ACHJWB_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          309 a          277 c          210 g          392 t
ORIGIN      
        1 tgttgtgatt tatacttcag tttcatcagc ttttcttcac ttaaatcata ggtcattgct
       61 gtagatccca tcacactttc gttttctttt attttcacat cagcaccatg caattgacca
      121 ataagagaaa aataaaaaaa tagtaacaag catgctttca tacatctaca acctatctta
      181 tatcactggg tttttctggc gtaccttgca tattagaaat ggggtgccca gatggcccat
      241 atgttatcca attaccaagg tcttttgttg ttatagccat tagatcctta tttgaattaa
      301 ggttcgttgg tgctgttagc actgtatcca tttttaactt accattagtt cctcgccccc
      361 accacgtcca tagcgtgtca ggtcctatta catttgtatg taactcttta gctaattggc
      421 tagcaaatga attttgtcct ttacctgtat tacaagaaaa taaccaaact gtcatcccat
      481 ctttataatt cccatcaaat ttaattagat atgctagatc tttagctgtc atgatactgc
      541 gcgttgcgga ttcaatagat gtgggggtac catgcccccc gattgtgaaa acgcctggga
      601 tattaatctc atcagctaca gaatggataa gatcactttc ggggtataaa tttatatcaa
      661 ccaaaccttg cggatctaca tttatgaccg gattcaacgg atactgataa aaattccatc
      721 cccccttcag tccaattggg tcctgagtga tatatcgccc ctgcagcggg tcataatagc
      781 ggtggcggtt gtaatacagg ccggactcct catcatactg ctgccccggc aaacggataa
      841 gctgctgcag ctggtgcggg ttctcttcat tcagcaggtt gccccactca tcgtattctg
      901 cgcaccattc tgttgcccct tccgtgctga taagctccag cggcaggtta ttcggtttgc
      961 gtcgttgagt tcactacact aaaactccgt gcccatataa cccaagtcgg aggcatgcct
     1021 tcctctggtt cgttccatct aaaatgtcta tacataacat ggttattcaa ttattttttc
     1081 tttgaattta ttctcaatcc agctgtgttt atacctgatg aaaatttgac actcaccaat
     1141 attatcaatg tctccagaat ttgtatttgg aatttgtcca actgcaac
//
LOCUS       DAZFAH010000436         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFAH010000436 DAZFAH010000000
VERSION     DAZFAH010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.439"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            131..733
                     /gene="ais"
                     /locus_tag="ACHJWB_005090"
     CDS             131..733
                     /gene="ais"
                     /locus_tag="ACHJWB_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4316520.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(772..>1186)
                     /gene="nudI"
                     /locus_tag="ACHJWB_005091"
     CDS             complement(772..>1186)
                     /gene="nudI"
                     /locus_tag="ACHJWB_005091"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4316521.1"
                     /translation="TIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEE
                     ALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVK
                     INEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          320 a          270 c          252 g          344 t
ORIGIN      
        1 ggcaaaattt tgaggtattt tgccgacatc gtaggatttt taatattaca ccaactgcta
       61 attatcgcca gaaatgtcgc tcaattttac ggtaattgtc tggttgcgct tgtctatagg
      121 tggagtttac gtgttagctt tttgccgctc atcattgaag tcaaaaaaat atttcatcat
      181 tttactggcg ctcgctgcaa ttgccggact gggtactcat gccgcctgga gtagcaatgg
      241 tttgccacgt atcgacaata aaacattggc cagactggca cagcagcacc cggttgtcgt
      301 tttgtttcgc catgctgaac gttgcgatcg ttcaaccaat caatgcttgt cagataaaac
      361 aggtattacg gttaaaggta cccaggatgc ccgtgaactg ggcaacgctt ttagcgctga
      421 catccctgat ttcgatcttt attccagtaa taccgtccgg accattcagt cggcgacctg
      481 gttttccgcg ggtaaaaagc tgacggtaga taaacgactt cttcagtgcg gtaatgagat
      541 ttatagtgca attaaggact tacaaagcaa agcgcctgat aaaaatatcg ttattttcac
      601 ccataatcat tgcctgacat atattgctaa aaataagcgt gatgcgacat ttaaacctga
      661 ttatctggat ggtttagtca tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt
      721 cgttaaccac taaattttca atctgacagc cagtgatggc tgtcatcatt gttaaagaag
      781 acctttcaaa cgtaacgttt ttcgggtggc aacatttaaa tcataatgca ccagatcttc
      841 aggttttacc cacgcgtagt cctgaaactc ttcgtttatt ttcacttctc ggttggcaga
      901 aacgcagtca aaaatcaggt aaatcatata aatctcttcc ttgcgaccat ctgcatacgt
      961 cttggtgcga atatcatcgc tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc
     1021 tcccagttct tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc cagactccac
     1081 gccgccaccc gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg ccattttaca
     1141 cagcaaataa gcaccatcat tttgaatcaa agggcataca atagtc
//
LOCUS       DAZFAH010000437         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFAH010000437 DAZFAH010000000
VERSION     DAZFAH010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.440"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..845)
                     /locus_tag="ACHJWB_005092"
     CDS             complement(<1..845)
                     /locus_tag="ACHJWB_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4316522.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCV"
     gene            822..965
                     /locus_tag="ACHJWB_005093"
                     /pseudo
     CDS             822..965
                     /locus_tag="ACHJWB_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1011..>1183
                     /locus_tag="ACHJWB_005094"
     CDS             1011..>1183
                     /locus_tag="ACHJWB_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316523.1"
                     /translation="MAKHGAGCLPVNSVSAPESAIIPYTWLLIAPPHRGIHHAEVFLI
                     NSKQKNQALCRLFL"
BASE COUNT          287 a          344 c          283 g          269 t
ORIGIN      
        1 acgcactgac ctgccatatc cgtcaggtct gcacggaatt tatcaaccag cgcaccaaac
       61 tgtgctgcgt gatttccggg cgtgccgtca aaatcaaatt ccccctgcat ccacaccacg
      121 gcaaacagca catttttcgg gttcttcttc agtgctgctt ttgttcgacc gataaggtcc
      181 ttatacagcg gcttgtccac accccagcgg gttgaattct ccgaggcacc actcgcgtca
      241 ctgtatgtgc catcagctcc ggtggtgaac gctgaaccac cacgacagca cggaaccagc
      301 agaatgcccg cattcgccgg tataaacggc agcagttttt tggcgatatg cagcccctgc
      361 cccacggtac cgtactgccc ttttgacagg tccgctttcg gatggttaag acggctcatg
      421 tcctgcacat catgcagaca atggtccgcc ggaatgatgt cgttatattt gcatactgca
      481 ccgcccggtg tcaccgtact gcggcgcgcc agctgcttaa tacgcgggtc cggacggtca
      541 tatgtctccg gcagcggaag gccttcacca tatgccatgc tgtttgactg ccccgccaga
      601 accacaacaa agtaatactc cgtgtctctg gtggcgctga ttactgtgcc ttctccatcc
      661 gacggcttca ccaacacagg tgtggtgaca tcaccttccg ccgcaatggc ctgcatcaga
      721 gtataaggcg tgatggccac cggactacca aacggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata atgtttaatc
      841 gacatcgaat ttctcccgtg tacaggaaca gagttaaaaa gccggaaccg gaatcaaatt
      901 acaggatggc catctgccag tggctggtcg taaaaaaaag gccacgccat gcgcagccgg
      961 aaataaaggg ataacgatga tagtttgaga aaaacagaaa caacactttt gtggcaaagc
     1021 atggcgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg attatcccat
     1081 atacctggtt gctgatcgcc cctccgcaca gggggattca ccatgcagaa gtgtttttaa
     1141 taaacagcaa acaaaaaaat caagcattat gcaggctgtt tct
//
LOCUS       DAZFAH010000438         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFAH010000438 DAZFAH010000000
VERSION     DAZFAH010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.441"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..202)
                     /locus_tag="ACHJWB_005095"
     CDS             complement(<1..202)
                     /locus_tag="ACHJWB_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4316524.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFS"
     gene            complement(156..356)
                     /locus_tag="ACHJWB_005096"
     CDS             complement(156..356)
                     /locus_tag="ACHJWB_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4316525.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            471..935
                     /locus_tag="ACHJWB_005097"
     CDS             471..935
                     /locus_tag="ACHJWB_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316526.1"
                     /translation="MIQGGDYLLKENEELGGENDHKMIILLTFYTVKVGSRVYVVCFK
                     STGYYIKVKPSIAGTMLGQSVKTCINALLRARGVSYDIPRSPVKQRCIKRRSLSWMKE
                     PVCPACKTPLQLIYRRSRSQNGKTYKPKNASCFRMLICPACSPRRENTQNVH"
     gene            complement(974..>1183)
                     /locus_tag="ACHJWB_005098"
     CDS             complement(974..>1183)
                     /locus_tag="ACHJWB_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4316527.1"
                     /translation="VKQALYSEEVNRVLKAQVRKDFEAQIDAYVDEVLARMVGRSPAE
                     NSTENDPQPVEQPEPVQPGTDGTMM"
BASE COUNT          320 a          262 c          267 g          334 t
ORIGIN      
        1 ctgaaaatat gcccagatga aggtaggagt tatttacgaa acggtggctg taatgtttat
       61 tgacgatgac cagtctggcc agccagcaac ttattgtctc aacccgcaac tcacgggagc
      121 cataaccaac gatattgtcg ttatagcgaa taagttcagg cgtgctgatg attcgggatg
      181 tgacttgctt tctgttcccc acgacaggat ttccttgtgt atgtggggtg ctctgtggcg
      241 ctcggagatg tgatttgatt gagggtttta cagcgcggac attttacttc cagataactg
      301 aaactggccc gtgccagtag cttattgcag tgtcggcacc gtatgtttcg atacatgacc
      361 gcataacctc ctgctttgtt gttgatgcct ttctcatgca atcgatcgac aaaaacgatc
      421 gattcgattt gtttaattga tacaacaaat agattgaaaa gtcatcaaca atgattcagg
      481 gaggtgatta tttacttaaa gaaaacgaag agttaggtgg tgaaaacgat cataaaatga
      541 tcattttatt gactttttat acagtgaagg taggatcacg cgtatatgta gtatgtttca
      601 aatcaacagg atactacata aaagtgaaac ccagcatagc tggaactatg ctgggtcaat
      661 ctgtaaaaac ttgcatcaat gctttacttc gagctcgtgg cgtatcctat gatattcccc
      721 ggtctcctgt aaagcagaga tgcataaaaa ggagatcttt atcatggatg aaagagcctg
      781 tttgccctgc ctgtaagact cccttacagc tcatctatcg ccgttctcga tctcaaaatg
      841 gcaagacata caaacccaaa aatgcttcct gtttcaggat gctgatatgt cctgcctgtt
      901 ctcccaggag ggaaaataca caaaacgtgc attaacaaac tcacccggca gggaaacctg
      961 ccggtcggta ttgttacatc atagtaccgt cagttccggg ctgaaccggc tctggttgct
     1021 ctacgggttg aggatcattt tccgtgctgt tttcagccgg agaacggcca accattcggg
     1081 ccagcacttc atcaacatat gcgtcgattt gtgcctcaaa atccttgcga acctgcgcct
     1141 tcagcacgcg gttcacttct tcagaataaa gtgcctgttt gac
//
LOCUS       DAZFAH010000439         1178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFAH010000439 DAZFAH010000000
VERSION     DAZFAH010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.442"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..489)
                     /gene="sfmF"
                     /locus_tag="ACHJWB_005099"
     CDS             complement(<1..489)
                     /gene="sfmF"
                     /locus_tag="ACHJWB_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4316528.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KAD"
     gene            complement(500..>1178)
                     /gene="fimH"
                     /locus_tag="ACHJWB_005100"
     CDS             complement(500..>1178)
                     /gene="fimH"
                     /locus_tag="ACHJWB_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4316529.1"
                     /translation="VDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIR
                     PFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFG
                     DIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQ
                     DLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALG
                     YLRVDYQ"
BASE COUNT          321 a          315 c          268 g          274 t
ORIGIN      
        1 atccgctttc gccacacctg gccgaacacc gctggctaac gctcgataat tggcaaaaaa
       61 gtttagtgtg acattgccat ttgcatctac ttcctcagtc gggctcgccg ctcccagtgc
      121 gagccgggag cgatcgctat tacgtaattc gatggcgatg gtttgcgcca ttgcgggatc
      181 atccagagcc agcaggttgg tatcggatgc cggcgttccc gtaaataaaa tcgcaactga
      241 acccggagga catccctcca gccgcaggct aaaagggacg agtgccgtgg tatcgccagc
      301 gttcagtaat tgtgtcgtag gccatctgcc taaatctacc gtcttatcaa tatccgcagt
      361 gtttacggta caggagaaat caacaacgtt accgtgcaaa ttgatattaa tcgttcccaa
      421 agggtcagct gcccatccac tggaactcca cagtaacccg cagaaacagc taaagagtac
      481 tcttctcatt ctccgtacct cattgataat cgacgcgtaa ataccccagc gcgctaaacg
      541 gcccttcggt cggtttttga ccggtaatac tgataggcca ggcgcgaagt gtgacattgg
      601 ctgccgcagc tgcatccaga cggaaaggaa taacgctatt gagatcgtta ggcgtgatcg
      661 gcgtatcgtt ctgatcggcg acaataaaac ctaaatcctg attgtccgac accatcgcct
      721 gaccagaaac ggcactggct tccagacgca ttgttaaata agcctgcgca gcaacattcg
      781 tacatttgac cgcaatgctc ttggtttgcg gcatgacacc agcaggtcga ttacccggcc
      841 ctgccgcact aaataacgat gcgccgatat caccaaaatc aaattcaaca atctgcccgg
      901 catttaattc gcagttttgc ggtacttcaa cccggccacc aaaactaatg gtataaacag
      961 gtgtaaccaa cggatcgtaa ggcgttgatg tcacatacac ggtaaacatc acctgtctgg
     1021 ggatctccac catgttaata aagggacgaa taacctttaa acgaaatatt aaacgagaat
     1081 ccatcacccc gaatggaagg ttttgtgaaa cgttaggatc tgttcccatc cgaatataat
     1141 tttgcggggg gaactgaata tccatcacac tatcaacc
//
LOCUS       DAZFAH010000440         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFAH010000440 DAZFAH010000000
VERSION     DAZFAH010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.443"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            229..926
                     /locus_tag="ACHJWB_005101"
     CDS             join(229..478,478..926)
                     /locus_tag="ACHJWB_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4316530.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            943..1175
                     /locus_tag="ACHJWB_005102"
                     /pseudo
     CDS             943..1175
                     /locus_tag="ACHJWB_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
BASE COUNT          315 a          262 c          288 g          310 t
ORIGIN      
        1 ctgttctgat acaggaaaat aaacgttcat tctatggtta cctcttcctg ttatatagaa
       61 gaattttaaa cgacaagtaa gggaatataa atgaaaataa caaaaaataa aaatgcaact
      121 ataaaaaaca gtcatcagtc ggctgctttt caatgtttac cttatcattt tctggtaatg
      181 ctgccaactt actgatttag tgtatgatgg tgattttaag gtgcttgcgt ggcttccatt
      241 tccatcagat gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg caaaagcact
      301 gccggacatc agcgctatct ctgctctcat tgccgtaaaa catggcaact acagttcact
      361 tacaccgcct ctcagcccgg taagcaccag aaaatcattg atatggccat gaatggcgtc
      421 ggatgtcgcg ccagtgcacg cattatgggc gttggcctca acacggtttt acgtcactta
      481 aaaaactcag gccgcagtcg gtaacctcgc gcatacaacc gggcagtgat gtgattgtct
      541 gcgctgaaat ggacgaacag tggggctacg tcggtgctaa atcacgtcag cgctggctgt
      601 tttacgcgta tgacaggata cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc
      661 tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga
      721 cggatggctg gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc
      781 gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctag
      841 gacggaagtc actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt
      901 atctgaacat aaaacactat cagtaagttg gagtcattac ctgagaataa tacctcctgg
      961 gtaagtgagc agatactttc agcgcctccg aaagatgtaa atattcgact tggagtcggt
     1021 tctttagaag gtacaaccgt ctcacatgtt cagcgattgc atcattcgtt aatcgcagct
     1081 ggtttggaaa gtaacctcac tgtctatacc ggtggtcacg attatgcctg gtggcgcgga
     1141 gcaattattg atgaattagc aaattataat tgcag
//
LOCUS       DAZFAH010000441         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFAH010000441 DAZFAH010000000
VERSION     DAZFAH010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.444"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..768
                     /gene="sdiA"
                     /locus_tag="ACHJWB_005103"
     CDS             46..768
                     /gene="sdiA"
                     /locus_tag="ACHJWB_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4316531.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKSFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMTLIRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(836..1060)
                     /gene="yecF"
                     /locus_tag="ACHJWB_005104"
     CDS             complement(836..1060)
                     /gene="yecF"
                     /locus_tag="ACHJWB_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4316532.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1047..>1169)
                     /gene="yecU"
                     /locus_tag="ACHJWB_005105"
     CDS             complement(1047..>1169)
                     /gene="yecU"
                     /locus_tag="ACHJWB_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4316533.1"
                     /translation="GTLKKQALLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          301 a          259 c          281 g          328 t
ORIGIN      
        1 tatcattata aatgatactc actctcaggg gcgttgcggt ttactatgca ggatacggat
       61 tttttcagct ggcgtcgcac gatgctgttg cgttttcaga ggatggaggc cgcagaagag
      121 gtctaccatg aaatagagct tcaggctcag cagctggagt acgattacta ttcgttatgt
      181 gttcgccacc cggtaccatt cacgcgacct aaagtggctt tctacaccaa ttaccctgag
      241 tcgtgggtta gttattatca ggcaaaaagc tttctcgcaa ttgatccggt gctgaaccct
      301 gaaaacttca gtcagggcca tttaatgtgg aatgatgact tattcagcga agcacagccg
      361 ttatgggaag ccgcgcgtgc gcatggttta cgccgcggtg tcactcagta tttaatgctg
      421 ccaaaccggg cactgggctt tttgtccttt tcccgttgca gcgcgcgcga aatacccatt
      481 cttagtgatg aactgcaatt aaaaatgcag ttactggtgc gcgaaagtct gatgactctg
      541 atacgtttaa atgatgaaat agtgatgacg ccagagatga atttcagcaa gcgcgaaaaa
      601 gaaattctga aatggacggc ggaaggtaag acatcagcag agatagcgat gattttgtca
      661 atctctgaga atacggtcaa tttccatcag aaaaacatgc agaaaaaaat taatgcaccg
      721 aataagaccc aggttgcctg ttacgcggcc gctactggct taatttgatc tcttttctgt
      781 cctgcgtgcc agatgcaaaa accggctgaa aggcacgctc tcagccggtt ttatattact
      841 gacggtaggc ttgtttaatt tgcttaaccg ttttggaaaa tactgcagcc tgggtttcgt
      901 cttcgatcag cgcaagctgt ttttccatct ttaacatcac gcggcccgcg tcagcttgtc
      961 ccatcgcctg cagcatcagc gtcagcatcg ctttcaggca ggagacttca tttgccagtt
     1021 cttgattatt ctcggcagta gaaaaatcag gcgtactcat ttattttcct cgtcatgttg
     1081 caatgaaaat ttgcggtgaa aaatgttaag gcggcggagt ataccataag ctttgctaaa
     1141 aatagcagtg cttgtttttt gagcgtgcc
//
LOCUS       DAZFAH010000442         1168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFAH010000442 DAZFAH010000000
VERSION     DAZFAH010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.445"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..231
                     /gene="hokD"
                     /locus_tag="ACHJWB_005106"
     CDS             76..231
                     /gene="hokD"
                     /locus_tag="ACHJWB_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4316534.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYESEE"
     gene            442..621
                     /locus_tag="ACHJWB_005107"
     CDS             442..621
                     /locus_tag="ACHJWB_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316535.1"
                     /translation="MSYALKKHPRLTIPPRDKSVVAAPRPAIDENCTHREQVKNAFDF
                     GFSRYEKAMEELSKV"
     gene            640..1125
                     /locus_tag="ACHJWB_005108"
     CDS             640..1125
                     /locus_tag="ACHJWB_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO4316536.1"
                     /translation="MAEIVEGVHYLSVDDLTEINRLLIELQTPDEPVGVLSPDNLSSS
                     QARPSMVRWYEQINDMFVLASVLIESLIQNHPFANANKRTAMMAGYVFLLLNGYELTA
                     PGDEIVEMAEGLACKTYTREDLENWLCYWSRAYDSRELCKTGATIVLYETIKLKIEQQ
                     N"
BASE COUNT          321 a          247 c          290 g          310 t
ORIGIN      
        1 taatctgagg ccatatctat gacttgcaca tgtaggttag cctcttacgc gccgaaaggc
       61 aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt
      121 ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga
      181 accggccaga cggaggtcgc tgtcttcaca gcttacgaat ctgaggagta agagaccagg
      241 cgggggagaa atccctcgcc acctctcatg tgtcaggcat cctcaacgca cccgcactaa
      301 acccgcttcg gcgggttttt tcacgtactg tggttgtgaa tacgattggt attaggctat
      361 gctaacaaca ttagcctgac taattttgta ttgacttgat tttttgttaa agaacaggac
      421 ggagaagtaa gggtacaccc aatgagctac gcactaaaaa aacacccgcg attgactatt
      481 ccccctcgcg ataaaagcgt tgtggcagct ccgcgcccgg ctattgatga aaattgcacg
      541 catcgtgagc aggtgaaaaa tgctttcgat ttcggttttt ctcgttacga gaaggcaatg
      601 gaagaacttt caaaagtgta ataatgggta ttgtgctcta tggctgagat tgttgaagga
      661 gtgcactatc tttcggttga tgatcttact gaaatcaatc gccttttaat tgagcttcaa
      721 acaccagatg aacctgttgg tgttctgagt ccagataact taagttcttc tcaggcccgt
      781 cccagcatgg ttcgatggta tgaacagatc aatgacatgt ttgtactggc atcggtattg
      841 attgaaagtc tgattcagaa tcatccgttt gctaatgcga acaaacgaac agctatgatg
      901 gctggttacg tcttcttgtt gttgaatggc tatgagttaa cagcaccagg cgatgaaatc
      961 gtggaaatgg cagaggggct ggcctgcaaa acctataccc gagaagatct cgagaactgg
     1021 ttgtgttatt ggtctcgcgc gtatgacagc cgggaattat gtaaaacagg cgcaactatt
     1081 gttttgtatg aaactatcaa gcttaaaata gaacagcaaa actaaaggtg cttccaatga
     1141 aaacccgctt cggcgggttt tttgttgc
//
LOCUS       DAZFAH010000443         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFAH010000443 DAZFAH010000000
VERSION     DAZFAH010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.446"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..979
                     /locus_tag="ACHJWB_005109"
     CDS             <1..979
                     /locus_tag="ACHJWB_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4316537.1"
                     /translation="WLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVM
                     ASPENFRFSEERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAIT
                     GDPGLTGMNPVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELG
                     TAALSALTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALR
                     TEYVALSAQHGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPV
                     AALTGLQTDTVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            979..>1166
                     /locus_tag="ACHJWB_005110"
     CDS             979..>1166
                     /locus_tag="ACHJWB_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4316538.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGS"
BASE COUNT          263 a          344 c          331 g          228 t
ORIGIN      
        1 ctggctgatc aaccatgcag ccgttgagcg catggtctcc cgtgttgtgg cgctcaatca
       61 gccggtgaaa attgactaca accaccagac cctgattgag ggacatccgg caccggctgc
      121 cggttttgtg atggcctcac cggagaattt tcgcttcagt gaagagcgag gtttcgaggt
      181 gcgcccgaag tggaaccctc cggcccttga acatctgcgt aataacgaat tcccctggtt
      241 ttcaccggtg attggctatg acgagagcac cggcgaacct gtcgaacttc ggatgctggc
      301 tattaccggt gaccccggtc tgaccggcat gaatcctgtc gccgcactgt cggcggatga
      361 cctttataac gccttaaacc ctcctttaaa ggatacctcc atgaatgagc aattacgcca
      421 gttgctgacg gcgctcggtc tgaccgtggc tgacggtgac gaatttacgc cggagctggg
      481 cacggcagcg ctgtctgccc tcaccgggat caaaacccgt gcggatgcac acgacaacct
      541 gaaaacacag gtcgccagtc tgtcggcaga gctggaaact gcaaaaggca caccggctgg
      601 cggcagtatt gacctgacga aatacgtgcc cgttgaaacg tataacgccc tgcgtaccga
      661 atacgttgcg ctgtcggcgc agcacggcag caccacgctg gagcagttgc tggacaaggc
      721 cgagtccgaa ggacgcatct tcaaaagtga acgcggctat ctggaacaac tgggcgggca
      781 gattggcgtg gcggcacttt ctgcacagct tgacgcccgc cagccggttg cggccctgac
      841 cggccttcag accgacaccg tgaccgtgcc ggataaaaag actgccaccg ctgtgctgtc
      901 ggctgaagat atcgcggccg ccaaactgct gggtaaaacc gaagccgagt tcctgaaaat
      961 gaaagaggaa atgcaataat gcctaccccg attacaccgg cgatgatcac cgccctgatg
     1021 acgggctacc gttctgattt tcaggccggg atgtccatgg ccccgtcgca gtacaaaaaa
     1081 attgcgatga ccgtgccctc cacatcgaaa tccaacacct acggctggct ggggcagttc
     1141 ccgcagttcc gtgagtggat cggctc
//
LOCUS       DAZFAH010000444         1163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFAH010000444 DAZFAH010000000
VERSION     DAZFAH010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.447"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..1119
                     /locus_tag="ACHJWB_005111"
     CDS             58..1119
                     /locus_tag="ACHJWB_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4316539.1"
                     /translation="MKRKVLAMLVPALLVASAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDENNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDEVA"
BASE COUNT          355 a          222 c          273 g          313 t
ORIGIN      
        1 tggataacgg cacttattga tatattcatg aagattataa ttacaaggga atacataatg
       61 aaaagaaaag ttctggcaat gctggtcccg gcgttattag ttgctagcgc agcaaatgcg
      121 gctgaaattt ataataaaga tggcaataaa gttgatttct acggcaagat ggttggcgaa
      181 cgcatctggt caaatactga tgataataac agcgaaaacg aagatacctc ctatgctcgt
      241 ttcggtgtta aaggtgaaac ccaaatcacc agcgaactga ctggttttgg tcagtttgaa
      301 tacaacctcg acgccagcaa gcccgaaggt gaaaaccaag aaaaaacccg tttaaccttc
      361 gccggtttga aatataacga gttaggatca tttgactatg gccgtaacta cggtgttgct
      421 tatgatgccg cagcttatac cgatatgcta gttgaatggg gtggcgattc ctgggcttcc
      481 gctgacaact tcatgaatgg tcgtaccaac ggtgttgcaa cctaccgtaa ctatgatttc
      541 tttggcctgg ttgatggtct tgattttgcg attcagtacc aaggcaaaaa cagcaaccgt
      601 agcactaaga aacaaaatgg tgacggctat gcgttgtctg tagactataa catcaatggt
      661 tttggtatcg ttggtgcata tagcaaatct gatcgcacca acgatcaagt tgccgatgga
      721 aacggttcaa atgctgaact gtggtcattg gctgcaaagt atgacgagaa caatgtctac
      781 gcagctgtaa tgtacggcga aactcgtaat atgactccgg gttctattga tacaggtgtt
      841 gccgatcgag aaggcaatac aataatgcgt gatcaactta tcaatgaaac ccagaacttt
      901 gaagcggttg ttcagtatca attcgacttt ggtctgcgtc cgtccttagg ttatgtgtac
      961 tctaaaggta aagatatcaa aggggtaccc ggacatagat atgttgacgc tgaccgtgtg
     1021 aactacattg aagttggtac ctggtactac tttaataaga acatgaacgt ttatactgca
     1081 tacaaattca acatgctgga taaagatgag gtagcctgag tttaacggac actccttcct
     1141 gaaatagaat ggcatcagaa gga
//
LOCUS       DAZFAH010000445         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFAH010000445 DAZFAH010000000
VERSION     DAZFAH010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.448"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..>1159
                     /gene="espG"
                     /locus_tag="ACHJWB_005112"
     CDS             54..>1159
                     /gene="espG"
                     /locus_tag="ACHJWB_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO4316540.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSLG
                     LWNPTYSRSERQSFQELLTVLEPVSPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKIFVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEIDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQP"
BASE COUNT          361 a          251 c          230 g          317 t
ORIGIN      
        1 atatgatact agttgccaaa ttgttcatta caaaccagat aggagaatct ctcatgataa
       61 atggacttaa taatgactcc gcatctttag ttttagatgc tgcaatgaaa gttaattctg
      121 ggtttaaaaa aagctgggat gagatgtcat gcgctgaaaa gttacttaaa gtacttagtc
      181 ttggtttatg gaatccaacg tacagtcgta gtgaaagaca atcatttcaa gagttgttaa
      241 ccgttttaga gcctgtatct ccacttccca atgaattagg cagagtatct gctcgttttt
      301 cagatggctc atccttaaga atttccgtca ctaacagcga aagtattgaa gccgagattc
      361 gcacaccaga taatgaaaag atttttgtgc tcctagagtc aaacgaacaa aataggttat
      421 tacaatcttt acccatcgat cgccacatgc catacattca ggttcatcgt gccttatctg
      481 agattgacct gactgatact acctcaatgc gcaatctact tggttttacg tcaaaactat
      541 caacaacctt gattccgcat aatgctcaaa cagatccgct ttccgggcct acaccattca
      601 gctctatctt tatggataca tgtcgaggac tcggcaatgc aaagctttca ctcaatggtg
      661 ttgatatacc tgcaaatgca caaatgttgc ttcgcgatgc actaggactt aaagacacac
      721 actcatcacc ttcccggaat gttatagatc atggtatttc tcgccatgat gcagagcaaa
      781 tagcaagaga aagcagcggc agcgataatc agaaagctga agttgtggaa tttttatgcc
      841 atccagaagc agcaacggcc atatgctcgg ctttctatca atctttcaat gtgccagcct
      901 taacgttgac acatgaaagg atctctaaag ccagtgaata caatgcggaa agatcgttag
      961 atacacctaa cgcttgcatt aacataagta tctctcaatc atcagatgga aacatttatg
     1021 ttaccagcca taccggggtt ctgataatgg cgccagaaga ccgccccaac gagatgggca
     1081 tgttgacgaa caggacttct tatgaagtgc cgcaaggtgt gaaatgtaca atcgatgaaa
     1141 tggtaagagc gctacaacc
//
LOCUS       DAZFAH010000446         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFAH010000446 DAZFAH010000000
VERSION     DAZFAH010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.449"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..498)
                     /locus_tag="ACHJWB_005113"
     CDS             complement(<1..498)
                     /locus_tag="ACHJWB_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4316541.1"
                     /translation="MITFTKEQLISHVSENVKAMKFAVKQTVFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVR"
     gene            complement(555..950)
                     /locus_tag="ACHJWB_005114"
     CDS             complement(555..950)
                     /locus_tag="ACHJWB_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4316542.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(966..>1158)
                     /locus_tag="ACHJWB_005115"
     CDS             complement(966..>1158)
                     /locus_tag="ACHJWB_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4316543.1"
                     /translation="SFTETQADELIFPSLRRANLALRRAKSDVQKWERVCAALRELNK
                     HRDIVRQITDSSRRVVSEK"
BASE COUNT          277 a          321 c          241 g          319 t
ORIGIN      
        1 acggactaca tcaaaaaact caccgtctcg atattgcgca taaagaacga atgcgccatc
       61 acataaaata attacgtgct gaccatcatc cggcattcgc tcactacagc ttatccaacc
      121 atccggagtt accggatagt tggttgacgt ttccgagatt tcccgaaaat tattggttga
      181 cgaaccctta ttttcccgaa agtttccagc ctgaagcatg gcggcgctgt ctggcggggc
      241 agcatatagc ggcacgtata tttccggttc cttatcagca ccgggttgct cttccagtga
      301 gaatgtcttt ccggtaaatc gattcatata aagcacgggc tctgcttcca gcgaagcctg
      361 agcaacaagg gccagtgcta aatccaactc aattgcctcg agagaatttt tgaatactgt
      421 ctgttttact gcaaatttca tcgcctttac attttcacta acatgactga ttaactgctc
      481 ttttgtaaaa gtgatcatct cattctcctt tgatgcgaat gccagcgaca attgaagcct
      541 gatagctaat tcactcacag taccgcctcc tgaaaattgc cctgatagaa cgccagtaca
      601 cgcagcataa cttcactctt ccggcactcg ccacagatta tgttctgttg tctgtcgtag
      661 cggcgtattt ctccgtctgg taactttcga atcaatgtct ggtcggttgc tttctccgct
      721 gccttacgcc atacgcgata cacctgttct gatgtaaaaa caccgtattt accgggcatg
      781 tataaatcgc cacaagccag tacatccaca aggcaacgtc tgactgaatg ccagcctgct
      841 cccgtcgctc tctccagttg tgatatcgtc atgcgttcat ttttgcgtac cagcccgata
      901 attcgggcct tcagttcttc acgctgttcg tgtgtaaaag gtttcgccat aagcgcctcc
      961 ggctatcact tttccgatac aacacggcgg gaagaatcag taatctgtcg aacaatatcc
     1021 cggtgcttgt tcagctcccg cagcgcggcg cagactcgct cccacttctg aacatcactt
     1081 ttcgccctgc gcagcgccag gtttgccctg cgcagggacg gaaaaatcag ctcatctgct
     1141 tgcgtttcgg taaacgat
//
LOCUS       DAZFAH010000447         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFAH010000447 DAZFAH010000000
VERSION     DAZFAH010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.450"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1062
                     /locus_tag="ACHJWB_005116"
     CDS             <1..1062
                     /locus_tag="ACHJWB_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO4316544.1"
                     /translation="GFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQT
                     AAKYLLYMYLATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPG
                     TSQGRGQRFINFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPG
                     RGRPVTPPLPPSPVPPSPSSGGAGFWERMMGRAGGLLSSVGSRMGLGRFSGFFRSAGG
                     LAGRLGGGALWAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIG
                     STVGSYLGNYLGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDD
                     TSGHGLDVNVWNGGNYGLW"
     gene            1049..>1149
                     /locus_tag="ACHJWB_005117"
     CDS             1049..>1149
                     /locus_tag="ACHJWB_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316545.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRRE"
BASE COUNT          198 a          306 c          408 g          237 t
ORIGIN      
        1 ggctttgagt atgcccgtga tgcagtgaca gggttttatc aggcgatcag aaaggtacgc
       61 gacacgcttc aggctctgcg ggatgcgggc tatggtgatg cgctggatcg cattggtcag
      121 ggggcacaga ccgccgcgaa atacctgctg tacatgtatc tcgccacccg tgccctgaaa
      181 atggcgcggg ctgtcggaac ggggattata cgtccgggtg ctgcattgct gggatacgga
      241 ttatcagcgg cggccttcct tacctcacct ttccggcgct ctcctccggc cggaacacca
      301 ccaggcacat ctcagggacg agggcaacgt tttataaact ttcttaccgg ggtaaatccg
      361 gcagccgttc agccagtact tgtaacaaac tggcccacag gaggactgtc cggcaccggc
      421 gcgaccgata catcctcagg cggacgtcag gggcggggcc gcaggaaacg cggaccgggg
      481 cgcggaagac cggtcacgcc tcccctgcca ccgtccccag tgccaccgtc tccctcttct
      541 ggtggggcag ggttctggga gcgcatgatg gggcgcgccg gtgggttact ttcgtctgtc
      601 ggtagccgca tggggctggg gcgtttctcc ggctttttcc gcagtgccgg tggactggcc
      661 ggccggctgg gtggaggtgc cctgtgggct ggcgcgatgg cagccccggt tctgctggat
      721 ggcagcgcca gtgccacaga caagggagaa gcggtcggct ctcttgcagg cagtattgcc
      781 ggtggtgcgc tgggggcggc agccggacct gtggggattg ccattggttc tacggtgggc
      841 agctatctcg gtaactatct ggggggatgg ctgactgaag cctggcagaa attacggggc
      901 agtgatgatg aaagcagtgg acaggcggta caaaaagcct ctgcccgtgt ggagctggtt
      961 gccccggaag ggtggcaggc tcgcagtatt gatattgatg acacgtccgg tcacggactg
     1021 gatgtgaatg tctggaacgg agggaattat ggcctctggt gacggtcgtg gtgcgttccg
     1081 tggtgtgccg ttcctcgtct ggcgtgagca gcgcgaacgt ggtgggcgaa acattgtccg
     1141 tcgggaata
//
LOCUS       DAZFAH010000448         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFAH010000448 DAZFAH010000000
VERSION     DAZFAH010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.451"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..144)
                     /locus_tag="ACHJWB_005118"
     CDS             complement(<1..144)
                     /locus_tag="ACHJWB_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012368889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4316546.1"
                     /translation="MTKYALIGLFAVCATVLCFSLIFRERLCELNIHRGNTVVQITLA
                     YEAR"
     gene            complement(490..699)
                     /gene="hha"
                     /locus_tag="ACHJWB_005119"
     CDS             complement(490..699)
                     /gene="hha"
                     /locus_tag="ACHJWB_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4316547.1"
                     /translation="MAKTKQKWLYQLRRCSSVNTLEKIICKNTYDLPDSEREAFNSAA
                     DHRLAELTMNRLYDRGPKEVWAYVR"
     gene            complement(759..995)
                     /locus_tag="ACHJWB_005120"
     CDS             complement(759..995)
                     /locus_tag="ACHJWB_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4316548.1"
                     /translation="MNGLRRSSRNAQCRNYQQAGSQAVRLEVRGRWREAAGAWRRAAH
                     MASRPEWQQFALQRAEHCRRRCRGNCSERQRCSH"
BASE COUNT          273 a          312 c          223 g          329 t
ORIGIN      
        1 ccgtgcttcg taggccagag ttatctgcac cactgtattt cccctgtgaa tattcagctc
       61 acacaaccgt tccctgaata tcagtgaaaa acacaacacc gtggcgcaca cggcgaacaa
      121 cccgataagg gcatatttcg tcataaactt tgacctctct gcaaggaaga ggctacaatc
      181 agtagtgttc aagtacttca tgcagacctc acgagttaat ggattaacga gtggggtctt
      241 cgcatttctg gcacctgcgt cttgcctgaa ccagaaaggc tcagacaccc gccgcgataa
      301 taccacattc ccgtttgctg accatgtacc tgcattttcc ggttattatt ataaaaacgc
      361 cggggacggg gaaaacttgc tttttcccgt ttccggggtt ggacaactga gcaacgcgaa
      421 ggcgtcagtt atttctgttt attacattct tctttatatt ttctttttac aaccagactc
      481 tgctttatat tacctgacat atgcccagac ctcttttgga cccctgtcat ataacctgtt
      541 cattgttaat tcagcaagac ggtggtctgc tgctgaatta aaggcctcac gctcactgtc
      601 cggtaaatca taggtgttct tacagataat cttttccagt gtattcaccg atgaacaccg
      661 ccgtaactga tataaccatt tctgttttgt tttcgccata tttatttcca gtcagcatga
      721 tattatatgt attaaaatta aacgacacag tctttcattt aatgactaca ccgctgtctt
      781 tcgctacagt tccccctgca gcgccgccgg caatgctccg ctctttgcag ggcaaactgt
      841 tgccattctg gtcgggaagc catgtgtgcc gcccgacgcc atgctccggc agcctctctc
      901 catcgccctc tgacctccag tctgaccgcc tgactcccgg cttgctgata attccggcac
      961 tgcgcatttc gtgaacttcg tctgagtcca ttcatttttc ctgccccctt ttactcttta
     1021 actttcccga taccagcagg actgaagtgg cacactgaat ttggccacct gaacagaggt
     1081 gatatgctca cctcagaaca acacaggtgc tccaatgaaa aaaagaaatt ttagcgc
//
LOCUS       DAZFAH010000449         1134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFAH010000449 DAZFAH010000000
VERSION     DAZFAH010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.452"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(122..334)
                     /gene="cspF"
                     /locus_tag="ACHJWB_005121"
     CDS             complement(122..334)
                     /gene="cspF"
                     /locus_tag="ACHJWB_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO4316549.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            635..850
                     /gene="cspB"
                     /locus_tag="ACHJWB_005122"
     CDS             635..850
                     /gene="cspB"
                     /locus_tag="ACHJWB_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO4316550.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT          356 a          204 c          248 g          326 t
ORIGIN      
        1 cggccaaagt acagcataag cttttaaagc caatcaacca gtcatcaaga cagacggggt
       61 tattcataaa aactctccat gtgtgatccg atggggcctg aaattaaagc tttaatatag
      121 ctcatgaaag gtaaacattg gcagctgaag ggccacgcag accatttatc cggcaaaatt
      181 ccacgcgtaa tccggtggta atttcttctg catcgcggag attgagcgct gaaacatgaa
      241 gctggacatc gatacgacca tcggatgggg tgataagacc cttgccgctt ttgccgtcaa
      301 aggttttgac aattcctgtc attttacggg acaaaaaaat tccttaatac tgataacttg
      361 gcgcactata cacacgttcc tgaagaaagc tatagttttt tgatggggtt gaagatggct
      421 ggatgtctaa aataaacatt gcttcatatg ttcaactatg cgttaatgat tgcgtcggtt
      481 tgaagaacag acgatatacg aagtagttta ctaaagcagt tctcatttca ggtgttattc
      541 acttattcct tctttgagtc tctccaatta agtacgaagt cgtttctgtt atgcaaacca
      601 tttatgccga aaggctcaag ttaaggaatg tagaatgtca aataaaatga ctggtttagt
      661 aaaatggttt aacgctgata aaggtttcgg ctttatttct cctgttgatg gtagtaaaga
      721 tgtgtttgtg catttttctg cgattcagaa tgataattat cgaaccttat ttgaaggtca
      781 aaaggttacc ttctctatag agagtggtgc taaaggtcct gcagcagcaa atgtcatcat
      841 tactgattaa aattcatcgc tcgtctgtat acgataacga agaaggctga tgcctgagta
      901 gagatacgga cagagtagtg aatattggat ctctttaata aaaagtaagg aggtccaata
      961 catgaaacaa tggctagcat atttggcaaa atcttaatca ggaaaagtat gctaaccatt
     1021 gtggtgaagt gcaggtttgc tgcatgaata gttttacagc agaagctaac tgctggcatg
     1081 gcaaaacaaa gtgcgtaagt ggatgactcc cacaaaaagc accacaatct caaa
//
LOCUS       DAZFAH010000450         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFAH010000450 DAZFAH010000000
VERSION     DAZFAH010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.453"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..417
                     /locus_tag="ACHJWB_005123"
     CDS             <1..417
                     /locus_tag="ACHJWB_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4316551.1"
                     /translation="TLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAGPSV
                     RVNEWFSAYAMAGVAYSRVSTFSGDYLCVTDSKGKRHDVLTGSDDARHSNTSLAWGAG
                     VQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            477..>1133
                     /locus_tag="ACHJWB_005124"
     CDS             477..>1133
                     /locus_tag="ACHJWB_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4316552.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRTSSTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPE
                     ALRRFEQMVEEAARHAGEAKKNAGEAETSARNAGISAGQAEESAANADTSAGDASESA
                     RQAAESAAAAKQSEEASSSSASAAAQKASESLQSAADAELSKKTAESAAGNAARDATT
                     A"
BASE COUNT          274 a          271 c          371 g          217 t
ORIGIN      
        1 acactgggga tggtgacgtc attcagctat gcaggagaca agaatcgcca gattacccgt
       61 tacagcgata cccgctggca tgaagattcc gtgcgtaacc gctggttcag cgtgatggcg
      121 gggccgtctg tacgcgtgaa tgaatggttc agcgcgtatg cgatggcggg tgtggcttac
      181 agccgtgtgt cgactttttc cggggattat ctctgcgtaa ctgacagcaa gggaaaaagg
      241 cacgacgtgc tgaccggaag tgatgacgct cgccacagca acacgtctct ggcgtgggga
      301 gctggcgtgc agtttaaccc gaccgaatcc gtggccattg atgtcgctta tgaaggttcc
      361 ggcagtggtg actggcgcac tgacggtttc atcgtgggtg tcggttataa attctgatta
      421 gtaacacagc gttatgacag cccgccggtt caggcgggct tttttgtgga gagggtatgg
      481 cagcagtaca aatatcaggc gtgctgaaag atggtgcggg aaaaccaata cagaactgca
      541 ccattcaact gaaagccaga cggaccagca gcacggtggt ggtgaacacg gtggcctctg
      601 aaaatccgga tgaagccggt cgttacagca tggacgttga gtacggtcag tacagcgtca
      661 ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg tatgaagatt
      721 ctcaaccggg gacgctgaat gattttctcg gtgccatgac ggaggatgac gtccggccgg
      781 aggcactgcg tcgctttgaa caaatggtgg aagaggcagc gcgtcacgcc ggggaggcga
      841 agaagaatgc cggagaggcg gagacgtcag cgaggaatgc cggcatatca gccggtcagg
      901 cagaagagag cgcggcaaat gctgacactt cagcagggga tgcatcggag tcagcccggc
      961 aggcggcaga aagtgcagcc gctgcaaagc agtcagagga ggcgtcctcg tcctcggcct
     1021 ctgcggccgc tcaaaaagcc agtgagtcat tacaaagtgc agcagatgct gagttgtcaa
     1081 aaaagacggc agaaagtgca gccggtaatg cagccaggga tgcaacgacc gca
//
LOCUS       DAZFAH010000451         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFAH010000451 DAZFAH010000000
VERSION     DAZFAH010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.454"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..1057
                     /gene="cysB"
                     /locus_tag="ACHJWB_005125"
     CDS             83..1057
                     /gene="cysB"
                     /locus_tag="ACHJWB_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4316553.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVWLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          290 a          265 c          268 g          309 t
ORIGIN      
        1 tttattatta aatcgtatta gtcacgccgc ctggtgtgac gaaaaaacga tgttctgatg
       61 gcgtctaagt ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt
      121 caatcataac ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat
      181 cagtaaacaa gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg
      241 caagcacctg acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt
      301 cctgtcgaaa gtcgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg
      361 ctcgctgtat atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa
      421 aggctttatt gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca
      481 aattgctgat gccgtctcta aaggcaatgc tgatttcgct atcgccacag aagcgctgca
      541 tctgtatgaa gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac
      601 tccggatcac ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc
      661 gttggtgaca tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg
      721 cgcagggtta acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta
      781 cgtctggtta gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga
      841 tcccgacctt gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg
      901 ttttcgccgt agtactttct tgcgcagtta tatgtatgat ttcattcagc gttttgcacc
      961 gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga
     1021 ggtcatgttt aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc
     1081 ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt acttatttct at
//
LOCUS       DAZFAH010000452         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFAH010000452 DAZFAH010000000
VERSION     DAZFAH010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.455"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..223)
                     /locus_tag="ACHJWB_005126"
     CDS             complement(<1..223)
                     /locus_tag="ACHJWB_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316554.1"
                     /translation="MHHIKTAANLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYAD"
     gene            complement(246..1085)
                     /locus_tag="ACHJWB_005127"
     CDS             complement(246..1085)
                     /locus_tag="ACHJWB_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO4316555.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT          377 a          239 c          195 g          305 t
ORIGIN      
        1 gatcagcata agaataaaat gtaggctcat gagtaataat gatattgaca ccttcaaaaa
       61 cagcctttct taaaacatcc attgaggcaa tccagcaggt cccaatttta ttaataagtg
      121 tcgcctcagc accaaaaata attttatcaa cagttggttg cgaaacggca accattgaat
      181 ttaaaaaaca attaagattt gcggctgttt taatatgatg cattcaacat ccttcaccgg
      241 tttcatcagc aataaaataa tttcaacatt tctggaaaac gttttgccca tgcagactca
      301 gagtgggtat cacctcccca tagatgataa tctatagtta cattgcgttg tttacggaga
      361 ctcacgcata atttttccgc gcctgaaata tagacttcag gaaagtcttc gcgcatacca
      421 ctggtttcca gcgtaccgac atcgagatat acagtaagat ctgcaaagat attgttttcc
      481 tgaataaaat tcagcattgc gctgtcattg aaccatagtg ccggcgaaaa aacgccaact
      541 tttgaaaaaa cgtctagacg aagaattgcg gcaaacaaac tgatgtacgc acccatagaa
      601 ctacctgcta tcgctgtatt attacgatct gggagagttc gaaaattgct gtcaatatag
      661 ttcttgagcg tattcacaat aaaatcaaca tacaacgcac cttttcctcc actataatcc
      721 acatcagggt cccagccagg taagggaaaa gatgtgtttc gcggccatgg agaatactca
      781 tccatacggc tgaatttacc atgagacgat gcattatcaa tccccacaat gataacaccg
      841 tcgtattggg aatttgggaa aaaagcatct accgcctcag gtatatccca tacatgtgga
      901 ccaaaagata cagatttatc aaataaattt tgcccatcgt gcatatacaa tacagggtaa
      961 ctgacatcag attcataata ggacgcaggt agtaatattc ttatttcgcg actacttgcc
     1021 aattgtggca tcggaaagga ttgcaacaca tgatatcgat tcccatagtt ctcaatatcg
     1081 ttcataattt aaattaacct caataaacaa aagaaa
//
LOCUS       DAZFAH010000453         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFAH010000453 DAZFAH010000000
VERSION     DAZFAH010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.456"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            251..760
                     /locus_tag="ACHJWB_005128"
     CDS             251..760
                     /locus_tag="ACHJWB_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4316556.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            732..>1107
                     /locus_tag="ACHJWB_005129"
     CDS             732..>1107
                     /locus_tag="ACHJWB_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4316557.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRN"
BASE COUNT          304 a          229 c          290 g          284 t
ORIGIN      
        1 tagatgataa ttattatcat tttgcgggtc ctttccggcg atccgacagg ttacggggcg
       61 gcgacctcgc gggttttcgc tatttatgaa aattttccgg gaaccatgtc cggtttctct
      121 gcaagttaac tacatgaaaa atataagaac gggctttccg tgaaccggac atgcgcaaaa
      181 aatggacata taaaccggac atgatcggtt ttgtgctaat tgtgaggtga gagtttttgc
      241 gaggtgagga gtggctacgc agactgaagt tgccaggcat ttaagtctga ccgatcgcca
      301 gcttcgcaga ttgcagaaat tgccgggtgc cccgatatca aataagcgag ggcaactgga
      361 tctggatgcc tggcgcgatt tttacatatc gtatctgagg agaagtaaaa acgatgtgcc
      421 tgatggcgat agcgaagacg actatgagga gaaattgctt attgccagat gggaactgac
      481 agcagaacag gctgttacac agcagctaaa aaatgaggtg tcaaaaggaa aactgattga
      541 caccggattc tgtatttttg ccctcagtaa gctggcaatg gcgttatcca gtacgcttga
      601 ttccatccct ttatccatgc agcgacagtt tcctgattta acaccgcgcc atcttgacca
      661 tctgaaaacc cttattgcga agggggcaaa tcagtgtgcg cgggcggggg ataaattacc
      721 ggatttactc gatgaatata tcagagcaac aactgaataa tatgatgagc gctgtcacaa
      781 cagcattaca gcccctgata agggcattgc cggtgacgcc agttgaatgg gctgatcaaa
      841 attattatct gccgaaagaa tcttcatatg gtgagggaga atggaaaacg ctgccattcc
      901 agatcgccat tatgaactgt atgggtaacg accaggttcg cacggttaac ctgattaaat
      961 ctgcccgtgt tggctataca aagatgttgc tgggggtggt cgggtatttt attgagcata
     1021 aatcccgaaa cagtctgctt tttcagccca cggattctgc cgctgaagat tttatgaagt
     1081 ctcacgtgga ggcgacgatt cggaacg
//
LOCUS       DAZFAH010000454         1094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFAH010000454 DAZFAH010000000
VERSION     DAZFAH010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.457"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(443..1015)
                     /gene="hdeD"
                     /locus_tag="ACHJWB_005130"
     CDS             complement(443..1015)
                     /gene="hdeD"
                     /locus_tag="ACHJWB_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4316558.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          373 a          248 c          216 g          257 t
ORIGIN      
        1 gcaattggat tgccagcttt cttttaacgc attgaaatta cctgagtaaa taacgcattc
       61 atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg
      121 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
      181 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac tacggaaaat
      241 atcagccatg ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa attagatgaa
      301 attcagtagg ttgaaatgat cactagcagg taattatttc aatgatagtg cgcaattgat
      361 ctacaacact gcgtagcgga gagagtatta atcggatcat agtcacatca agtgactatg
      421 atccgggtga caaccgggat aattattgct gcttaacgaa caaactggcg aagctgaaca
      481 ggctggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttacagaca
      541 ccatcggcgt tgcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
      601 gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
      661 ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctccggtg
      721 cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
      781 ccggccagaa attatgactg cggttgctga ataacccgac aataagcgca atacccgagc
      841 agattaataa tgcacccact actgtgctta aaatatcgcc agagacgaac gggaaactga
      901 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgtt
      961 ttttaagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaat
     1021 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa
     1081 gaatgcagtc gatt
//
LOCUS       DAZFAH010000455         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFAH010000455 DAZFAH010000000
VERSION     DAZFAH010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.458"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..337)
                     /locus_tag="ACHJWB_005131"
     CDS             complement(<1..337)
                     /locus_tag="ACHJWB_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO4316559.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLN"
     gene            complement(972..>1060)
                     /locus_tag="ACHJWB_005132"
     CDS             complement(972..>1060)
                     /locus_tag="ACHJWB_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4316560.1"
                     /translation="NGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          305 a          224 c          182 g          349 t
ORIGIN      
        1 gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa tagcatgtat
       61 aggattcgga aagtccagca tctattttgt acctcattat attagataag gatgtgagaa
      121 attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct gcggccttac
      181 aggcaaaata gcttagtgaa cacgcttttc gtccctcttt agtactggct gatattagtg
      241 cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt gcagtaactg
      301 cactcgcaga aatagagaaa aaaatacaat tgttaataac cttgccatat tttttagtcc
      361 gtaactgtta atgtgctatc tggctctgaa aatattaact catttaattt ttgcatgtca
      421 ttagataaac cagttataca accctcggaa cagaatcgag gtcctaaaga cggaccaaaa
      481 gcgtttaaac aactatgtat tgaaaatcca ttgcgattat aagttcctgt attaactgac
      541 ggtactaatc gtatgccgtc aggttttcta ttgcttgctc ccgggtcatt aacattccaa
      601 ctccataggc cttgaggtat tggccatcta tttataaatg tgtacaacgg ggattatttt
      661 tacattgcat attattacca gaagcgactg gaatactaag cacatcataa aaattatcat
      721 cacatggtat atctgaaaca cacgaaagca tacctaaagt tatgctgtat aaacaggtgg
      781 ctaatcccaa ggggatcgat tccagaaatc gggttcaacg gatactgata aaaattccac
      841 ctaccttcca gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg ttaatgttac
      901 cgataatagt tatagtacag ccctgattca tcatcatact actgctgcgg cagtctgatg
      961 aactattgct tttacggatt ctccttattc ttccttctat ccattcgctt tagctttttg
     1021 agctcttgaa gtttgctctt tgcatcaata ttaccgttat
//
LOCUS       DAZFAH010000456         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFAH010000456 DAZFAH010000000
VERSION     DAZFAH010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.459"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            608..1060
                     /locus_tag="ACHJWB_005133"
                     /pseudo
     CDS             608..1060
                     /locus_tag="ACHJWB_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          309 a          206 c          227 g          318 t
ORIGIN      
        1 tgcgaataaa aaatggttac tgtagaagcg gatttccacc acatgtcaac catgaatggt
       61 cagtgatgcg agacgactgg gatgatttgg tcgatgactt cttccggcga atactgatag
      121 aaacattgaa atattgtaag agaaagcgtt caaaattctg tggcacgtga aaaattctac
      181 agcgtcatca ccttattcag tcgaccttca aaataaacgt ttaacagaga caatcaccat
      241 ttggattgtc cgctgaggag gcgaacaatt tcctagacag ctttatcatg tcagcccaca
      301 ttgttggcgc tggcaatact aaggacaaac gatggggaag tagcttttta gcgttatttt
      361 cgggagtaaa aaacgtggta tctttccgta tgtcagttgt atcttgctgg tattcgtagt
      421 cgtgataagg catacaacga tatttatctt gacgttagtg aagaataagt tgttttgtga
      481 tctatgttaa tctgggatta caatccgggc ttgatacatc gcatcacgac ttttattctc
      541 ttcagaatag aatatgttac tccccgcttt acaggctcag gatttgaatt acaaattata
      601 cttttaagtg aacaataacc aagggtattt cttatttcct gaaaccaggc ttggcccatt
      661 tcgggatgag tttgaaaaaa ctcttgacgc atacgatagt gatgaaataa acgaaacaaa
      721 ttacgtcaac aaactcaggc gtcttgcctg acatgctcct gacatcattg atattcacgc
      781 tcatctggca tatgcatttg cgctctttat gctgcaattc tggcgaatgt tcatttacag
      841 cgtcatcagg atgccgttat actcattgat aaaatactaa catataaccc tgagaataat
      901 cagggaacac gctggttact gggggcggaa ttgttacgtg ctggcaagca tggcagggct
      961 ctgtacgtat tgacaggaca tgtcaatgaa tttcccccct actggtacga gcttgctctt
     1021 ttacatttta tgaatgaggc atatattaat gctgcaacgg
//
LOCUS       DAZFAH010000457         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFAH010000457 DAZFAH010000000
VERSION     DAZFAH010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.460"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1046
                     /locus_tag="ACHJWB_005134"
     CDS             <1..>1046
                     /locus_tag="ACHJWB_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4316561.1"
                     /translation="VGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTAGVVEQNL
                     SRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKDSFGG
                     MIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQSGLTA
                     DRMLTLSRAGQAAGLTFNQTSESLTALVNASVRGGEQFEAISQSVARFASASGVEVDK
                     VAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDAATKGF
                     DEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDEADKK
                     WQWYQSRSQRRGKTA"
BASE COUNT          220 a          266 c          348 g          212 t
ORIGIN      
        1 gttggtgatc ttgttattga cctgagtctg gatgcggtcc gtttcgatga gcagatgagc
       61 cgggtaaggc gtcatttttc cggactggag actgacgcca gaaaaaccgc cggtgtcgtt
      121 gagcagaacc tgagtcgtca ggcgctggct gcacaaaaag ccgggatttc cgtcgggcag
      181 tataaagcgg ccatgcgaac cctgcccgca cagtttacgg atatcgccac gcagcttgcc
      241 ggtggtcaga atccctggct gatcctgctg caacagggcg gtcaggtgaa ggactccttc
      301 ggcgggatga tccccatgtt caggggactt gccggtgcga tcaccctgcc gatggtcggg
      361 gtcacctcgc tggcggtggc gacaggtgcg ctggcgtacg cctggtacca gggggattcc
      421 acgctttcag cgtttaataa aaccctggtt ctttccggta atcagtccgg actgactgcc
      481 gatcgcatgc tgacgctctc cagagccgga caggcggcag ggctgacgtt taaccagacc
      541 agcgagtctc taacggcgct ggtgaatgcc agtgtgcgtg gtggtgagca gtttgaggca
      601 atcagccaga gtgttgcgcg ttttgcgtct gcatccggtg tggaggtgga taaagtcgct
      661 gaagccttcg ggaagctgac cactgacccg acgtcgggac tgatggcgat ggcgcgccag
      721 ttccgtaacg tgacggcaga gcagattgcg tatgttgcgc agctgcagcg ttccggtgat
      781 gaggccgggg ccttacaggc ggcgaacgat gctgccacga aaggctttga tgagcagacc
      841 cgtcgcctga aagaaaacat ggggacactg gagacctggg cggataaaac agggaaggca
      901 ttcaaatcga tgtgggatgc cattctggat atcggtcgtc ctgagtcctc agcggatatg
      961 ctcgccagtg cacagaaggc atttgatgag gcggataaaa aatggcagtg gtaccagagc
     1021 cggagccagc gccggggaaa aaccgc
//
LOCUS       DAZFAH010000458         1040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFAH010000458 DAZFAH010000000
VERSION     DAZFAH010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.461"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..422
                     /locus_tag="ACHJWB_005135"
     CDS             213..422
                     /locus_tag="ACHJWB_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316562.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(423..>1040)
                     /locus_tag="ACHJWB_005136"
     CDS             complement(423..>1040)
                     /locus_tag="ACHJWB_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316563.1"
                     /translation="PSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRDAFTGLYF
                     CASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAGNMFVY
                     DNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAEKRWNVAT
                     WLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          285 a          235 c          205 g          315 t
ORIGIN      
        1 cacaaccgga agcgcacgtt cgaagaaatc taacgacaag ccttctaagg gaaagagctt
       61 cgccgtacgc tttcgcgtta tgccctgact tttcagggaa atatcctttc agtaaactgt
      121 cagtaccgga ttcttatccg tgtccggcgc acgaccacac gtgacagcgt gttggtctcc
      181 atttttaacc cagaacctca atggaggata aaatgccaaa caaaaaaaga aatccgctta
      241 ttgaaaaaca gattgaatgc ctggtaaatc aactcaggca atcagggtta ttaaaaactc
      301 attcagagtt gaggctcaca gaatcagcat tcgacgataa attaaataat gtcctttata
      361 atggcattat tgattttaat cgttctgttg gtcgccgcgg ccctgctggt gtttccttat
      421 aattaccagt caatccagag tggaccgtgt tcagcgtaaa tataactgta cacatccaga
      481 ttatatttgt ggtctgttaa gaacaggccg caaatacatg ccgaagcttc cagtgcagcg
      541 gctctgttac tgaataacca tgtagcaaca ttccagcgtt tttctgcatc ccagtcttta
      601 tcaaggcctg ataccatgaa gaaaccgtta gtgttgccat caaataattc tgtttccaaa
      661 tttttaagca atgcctgatg gactcttgcc aggtattccg ccggaatttc gccacgaatt
      721 ctgatgagat tgtcataaac aaacatgttc cccgcatatg gcgatttttc tttcttgttt
      781 tttaaaccag catcatgagc aaactgatca atttcttctt ccgttggttt cgtattgatg
      841 ttttgcgctg tcgtttctgc aattttattt gccacactct ctgagtcgtg tttatttata
      901 gacgcacaga aatacaatcc ggtaaacgca tcgcgcacat tacgagccat attatcagtg
      961 tcttttttcg ttaccgattc caattcaagt tcgttcagac gatgacgaag tgtgtgtgct
     1021 gcaatctcct ggattgaagg
//
LOCUS       DAZFAH010000459         1040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFAH010000459 DAZFAH010000000
VERSION     DAZFAH010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.462"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..728
                     /gene="nanC"
                     /locus_tag="ACHJWB_005137"
     CDS             12..728
                     /gene="nanC"
                     /locus_tag="ACHJWB_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4316564.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            748..>1040
                     /gene="nanM"
                     /locus_tag="ACHJWB_005138"
     CDS             748..>1040
                     /gene="nanM"
                     /locus_tag="ACHJWB_005138"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4316565.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNS"
BASE COUNT          322 a          210 c          244 g          264 t
ORIGIN      
        1 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
       61 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
      121 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
      181 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
      241 atgttcaggt tgaagttaat tacgcgatta aacttgatga tcaatggacg gtgcgcccgg
      301 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
      361 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
      421 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
      481 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
      541 agaacgatta tcgctatgca aaccataaga aatgggcgac ggaaaatgca tttgttctac
      601 aataccatat gacgcccgat attacgccat acatagaata tgactacctg gaccgtcagg
      661 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
      721 aactgtagta gacaggagac agtcacaatg aataaaacaa taacggcgct tgctatcatg
      781 atggcttcat ttgccgcaaa cgcgtctgta ttacctgaaa ctcctgtgcc atttaaaagt
      841 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacggca
      901 tggtacaagc tggatacaca ggccaaagat aaaaaatgga cagcgttagc tgcattccct
      961 ggtggaccaa gagatcaagc aacctcggca tttattgatg gcaatctgta tgtgtttggc
     1021 ggcattggca aaaacagcga
//
LOCUS       DAZFAH010000460         1039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFAH010000460 DAZFAH010000000
VERSION     DAZFAH010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.463"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..279
                     /locus_tag="ACHJWB_005139"
     CDS             34..279
                     /locus_tag="ACHJWB_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4316566.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            356..571
                     /locus_tag="ACHJWB_005140"
     CDS             356..571
                     /locus_tag="ACHJWB_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316567.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            576..>1039
                     /locus_tag="ACHJWB_005141"
     CDS             576..>1039
                     /locus_tag="ACHJWB_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316568.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPDMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRS"
BASE COUNT          250 a          239 c          320 g          230 t
ORIGIN      
        1 tttcttaatc acatctttcg acgagaaaat cccatgtcag aaattacatc cctggtcact
       61 gctgaagcag tgaaggaagt cctgcgctct gaagaagtcc ggagcgcact gaaacagaaa
      121 cttcgccata acctggaagc gcgtcttgat gcagaagtgg atgccattct ggatgaactg
      181 ctgggcgcac cggcagctcc ggagccggaa ggcatcgcgg gtgaggggag tgcttcagat
      241 agcggtgacc ccacaccgga cagcgacatg atgatgtaag catgcgtcag ggaccatcgg
      301 tgtgtgccgg tggtctttat attgttgtga gcttccggat tgcgggagac ggggtatgta
      361 ccagatggaa aaaatcacaa caggtgtgtc atacaccacg tcagcggtgg gaacgggcta
      421 ctggttcctg cagttgctgg acagggtttc cccgtctcag tgggcggcaa taggcgtgct
      481 ggggagtctg ctgtttgggc tgctgacata tctgacgaac ctgtatttca agattaaaga
      541 agaccggcgt aaggcggcac ggggagagta agctgatgag caggaaactc cgctatggtt
      601 tatcggctgc cgttctggcg ctgattgccg caggtgcttc tgcgcctgaa atcctcgacc
      661 agtttctgga tgaaaaggaa ggtaaccaca ccacggcata ccgtgatggt gcgggtatct
      721 ggaccatctg ccgtggagcc acccgggtgg atggtaagcc tgtgattcct gacatgaagc
      781 tgtcgaagga aaaatgcgac cgggttaacg ctatcgaacg ggataaggcg ctggcatggg
      841 tggagcgtaa tataaaagtt ccactgaccg agccacaaaa agcaggtatc gcgtcattct
      901 gtccgtacaa cattggtccc ggtaagtgtt tcccgtcgac gttttacaga cgaattaatg
      961 caggtgatcg aaaaggtgcc tgcgaagcta ttcgctggtg gattaaggac ggtggcaggg
     1021 actgccgtat tcgctcaaa
//
LOCUS       DAZFAH010000461         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFAH010000461 DAZFAH010000000
VERSION     DAZFAH010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.464"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..379)
                     /locus_tag="ACHJWB_005142"
     CDS             complement(<1..379)
                     /locus_tag="ACHJWB_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316569.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPD"
     gene            complement(436..828)
                     /locus_tag="ACHJWB_005143"
     CDS             complement(436..828)
                     /locus_tag="ACHJWB_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4316570.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     CLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEAVL"
     gene            complement(844..>1036)
                     /locus_tag="ACHJWB_005144"
     CDS             complement(844..>1036)
                     /locus_tag="ACHJWB_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4316571.1"
                     /translation="SFTETQADELIFPSLRRANLALRRAKSDVQKWERVCAALRELNK
                     HRDIVRQITDSSRRVVSEK"
BASE COUNT          242 a          293 c          226 g          275 t
ORIGIN      
        1 catccggagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt attttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaagttt ccccgataga acgccagcac
      481 acgctgcata acttcgctct ggcggcactc acgacaaatt atgttctgcc gtctgttgta
      541 acgacgtatt tctccgtcag gtaactttcg aatcagtgtc gggtcagcag ccttctccgg
      601 tgtcttacgc catacgcgat acgcctgctc tgatggaaat accccgcaac cagagagcca
      661 gacatcacca ctggccgcaa gcgcaccaga taaacaacga atagcggtct tactgacacc
      721 cgttttatct gccagttgtc gaaaagtttc tcgtccgctc aggcgcacga attccacaat
      781 gcgcgccttc acttcttccc gctcttctgg tgtaaatact tttgccataa gcgcctccgg
      841 caatcacttt tccgatacaa cacggcggga agaatcagta atctgtcgaa caatatcccg
      901 gtgcttgttc agctcccgca gcgcggcgca gactcgctcc cacttctgaa catcactttt
      961 cgccctgcgc agcgccaggt ttgccctgcg aagggacgga aaaatcagct catctgcttg
     1021 cgtttcggta aacgat
//
LOCUS       DAZFAH010000462         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFAH010000462 DAZFAH010000000
VERSION     DAZFAH010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.465"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..812
                     /locus_tag="ACHJWB_005145"
     CDS             <1..812
                     /locus_tag="ACHJWB_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4316572.1"
                     /translation="IPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHD
                     QLNYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPG
                     GINITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIAN
                     SESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDFQEN
                     NIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            825..>1031
                     /locus_tag="ACHJWB_005146"
     CDS             825..>1031
                     /locus_tag="ACHJWB_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4316573.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIK"
BASE COUNT          330 a          208 c          206 g          287 t
ORIGIN      
        1 ccattccttt ctactggggg gatgatattg ccaaaacacg tcaccaaatt aacttatcga
       61 attcgacctc attttccaaa gatggctatt cctccaacaa tactggaatt actggcatag
      121 ccggtgaaca tgatcagtta aattatggta tatatgttaa tcagcaacaa caaaataatg
      181 atacctcgct tggtacgaat ttaagctgga gaactcccat cgccataata gatggcagct
      241 atagtcattc taaaaacgcc tggcaaagtg gtggaagtat tagtagtgga ttagttgtct
      301 ggcccggtgg tattaatata actaaccagt tatccgatac atttgcaatt ctggatgcgc
      361 ctgggttaga aggtgcacat attaatggac aaaaatacaa tcgaacaaac agcaaaggcc
      421 aggttgttta cgacctgatt atacctcatc gtgaaaacca tctggtactt gatatagcaa
      481 acagtgaaag tgaaaccgaa ttgcagggca atcgtcaaat tattgcgcct taccgtggag
      541 cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata caggcactgc
      601 gtcccgatgg ttcgccatta acctttggct atgacgtact ggatttccag gaaaacaata
      661 ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt cccactggca
      721 taaaagttgc tctcaatgat gaacagaact tattctgtac tattactttt caacacgtta
      781 tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag tggcttttat
      841 taatcacctt gagtctatat tcttttattg tgcaaagtgc tccctgtgca ttgaccaatg
      901 tcggtgaaca acgtggaaca tatatactca agccactgag tatgaaaggt aatctaaccg
      961 ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtac agacttgcaa gagacaacca
     1021 acgccattaa a
//
LOCUS       DAZFAH010000463         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFAH010000463 DAZFAH010000000
VERSION     DAZFAH010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.466"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..411
                     /locus_tag="ACHJWB_005147"
     CDS             <1..411
                     /locus_tag="ACHJWB_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316574.1"
                     /translation="LVKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGT
                     KHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGRGHVTLTF
                     NVSSAGANNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            479..>1027
                     /locus_tag="ACHJWB_005148"
     CDS             479..>1027
                     /locus_tag="ACHJWB_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4316575.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVID"
BASE COUNT          234 a          243 c          309 g          241 t
ORIGIN      
        1 ctggtgaaga cggtggggaa agcctttccc cggaataaca gttatgccag cgggacggta
       61 accgtcacag tttacgatga ccagggattc gaccggcaga ttatcattcc cccggtgctg
      121 tttcgcggga cgaaacacca gaatttcaac agcccgaatc agcagtcgta ctggtattcc
      181 acctgtaagc tgcaggtgct gaagaacggg gttgagattt tccatgaacc ggcaacggat
      241 gtcagccggg tgttctcatc ggtgatagat atgccggcag ggcggggtca tgtcaccctg
      301 acgtttaatg tgtcgtcggc cggtgcgaac aactggacgc cgacaacgta catcagtgat
      361 ttactggttg tggtcatgaa aaaatccacg gcagggatca gtatcagctg acggtttatt
      421 aacccggacg ggcacctcag gaggtgcctt ttttattgac tgaaaacgag aggtcatcat
      481 gcggcattta tacgcaacga tattattgtt tactaccctg ctggcaggaa ttgcctttcc
      541 tgcacaggct gaaagcggac acggtgcatt ttccgtggga tatgctcagg ttcacccggg
      601 cggcgtaccg gcattgtccg gtaccggtgc gcgtgcaggt gatttaaaag ggattaatgt
      661 gaaataccgt tatgagttca cggatcacct gggcggcatt gtcgcgctga gttatgcatc
      721 ggtgaagaaa agtgacacga tgaagacggg tgaaaatacc ttccattatg aaagcctgcg
      781 cggtcgttat gtcagtctga tggccggccc tgtctggcag ctcagtgagc gggtcagtct
      841 ctatggcatg gccgggatgg cgtacacccg ctggtctgac agtgttcagg attaccggcg
      901 tgatgaagtg aaaccggggt atgtgaagga gaccaccacc gccagtgatg gccatactgc
      961 gcgtcatctg tcgccggcct ggaatgccgg gattcagttc agtcccgtag agacggtggt
     1021 tattgac
//
LOCUS       DAZFAH010000464         1014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFAH010000464 DAZFAH010000000
VERSION     DAZFAH010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.467"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..832
                     /gene="exuR"
                     /locus_tag="ACHJWB_005149"
     CDS             56..832
                     /gene="exuR"
                     /locus_tag="ACHJWB_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4316576.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
BASE COUNT          281 a          264 c          241 g          228 t
ORIGIN      
        1 cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga
       61 aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcatcga
      121 acagggcgtc tatctggtag gtgataaact gcctgcagaa cgctttattg ccgatgaaaa
      181 gaacgtcagc cgtacggtag ttcgtgaagc catcatcatg ctggaagttg aaggctatgt
      241 cgaagtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc
      301 ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca
      361 gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat
      421 gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg
      481 ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat
      541 cgttgaaaaa atgtggaccc aacgtagcca taacccgtac tggaaaaaac tgcacgaaca
      601 cattgattcc cgtaccgtcg ataactggtg tgatgaccac gatcaaatcc tcaaggcgct
      661 gattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg cagcacctgg aaaacaccaa
      721 gatcatgtta ttcaacgaaa ccagcgacga cttcgagttc aatgccgacc gctatctgtt
      781 cgccgaaaac ccggtagtgc atctcgatac ggccaccagc ggcagtaaat gaaatacctg
      841 tccgacaggc gctgcgacgc gcctgtttgt ctcgtcggta agcgaaaagt ataaagtgtc
      901 agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac
      961 atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa tttg
//
LOCUS       DAZFAH010000465         1011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFAH010000465 DAZFAH010000000
VERSION     DAZFAH010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.468"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..503)
                     /locus_tag="ACHJWB_005150"
                     /pseudo
     CDS             complement(1..503)
                     /locus_tag="ACHJWB_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(463..582)
                     /locus_tag="ACHJWB_005151"
                     /pseudo
     CDS             complement(463..582)
                     /locus_tag="ACHJWB_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(595..>1011)
                     /locus_tag="ACHJWB_005152"
     CDS             complement(595..>1011)
                     /locus_tag="ACHJWB_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316577.1"
                     /translation="NYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAK
                     SAAIVYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSG
                     TLHEAPSPIIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          267 a          220 c          169 g          355 t
ORIGIN      
        1 aatgaatgga ccaatataat gattttttgt ctaacactgg agcgtttccg tattggcaga
       61 ctgtgacttt atctaaccaa agattcaatt cccctgttca tattcttatt agtggttgag
      121 cagggctcaa tactccatca aatactttac ctacgtgcaa acgattatat tttagttcag
      181 gatgctttag atttaacggg gaactggata aactgagcca ttcccctatt aacctttccg
      241 tttctggcat taaagccatc ccagcgagct cagatgaaat taagggaaat atttctccaa
      301 gcaataccca tccagaccag ctgccactct cttctgctat cggtaaccat ttagcactaa
      361 attcgggcat taataatgca acatcttcgc cattttcttt ccaattttga aaagtagttt
      421 caaacgtatg agtgtttcga tttactttcc tcagaccgaa cattaacttt catccttctg
      481 atcgttttcg aatttgcttt cattttcttg ctcctcataa ggctgatgtc gttgtccttg
      541 tcgttcatct tgctcagtca atacccaacg ttgtgctgta actcattatg aaaactattt
      601 aattccctca tcattgataa ggttggacat ctctttcaac atgagagtca ttgaagcttt
      661 cgaccaattt agcggaatga tagggcttgg cgcttcatgc aaagttcccg acttgtggat
      721 atagttatag ctttcgggaa gtgccatttc aggtacactc ctgccatttg caccaatcat
      781 attctcgcct gttatttggg ccaatgatct attcataaat tgcactgagt cattcaaata
      841 ctcttccttt cttgattctt tataaacaat tgctgctgat tttgcatacc atgagtcaaa
      901 aaaccattct gcttctgttg atggaatgaa gctcttttct cttttcgcat gtgaattttg
      961 atcggtatcg gttttaatat cattgaacca aaaatttgcc gactgataat t
//
LOCUS       DAZFAH010000466         1009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFAH010000466 DAZFAH010000000
VERSION     DAZFAH010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.469"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(292..924)
                     /locus_tag="ACHJWB_005153"
     CDS             complement(292..924)
                     /locus_tag="ACHJWB_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4316578.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          258 a          222 c          214 g          315 t
ORIGIN      
        1 tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt
       61 tacaagtaag ggtatgatta cgctttttaa agcgtaccat aaaacataac ataataaatg
      121 atttgtgatg acacgggatt attgggattt actttgagga agataaaatg cgtgtttata
      181 gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat
      241 tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac
      301 aaacagtgct tcgctgtcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc
      361 ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg
      421 tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc gaagttcttc
      481 cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc
      541 tttttcggga gttgctacat aactctcaga aattccgaca gatgcattaa tgacaatata
      601 ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc attcggtact
      661 gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ctggatcgcc
      721 atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga
      781 gatacgctct gctgctgccg ttttctgggt ttgctgaact tgctgagttt gctgttgtgc
      841 cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat
      901 cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg
      961 ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcat
//
LOCUS       DAZFAH010000467         1006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFAH010000467 DAZFAH010000000
VERSION     DAZFAH010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.470"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..>1006
                     /gene="phoE"
                     /locus_tag="ACHJWB_005154"
     CDS             13..>1006
                     /gene="phoE"
                     /locus_tag="ACHJWB_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4316579.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDS"
BASE COUNT          297 a          203 c          256 g          250 t
ORIGIN      
        1 tcaggaatga aaatgaaaaa gagcactctg gcattagtgg tgatgggcat tgtggcatct
       61 gcatctgtac aggctgcaga aatatataat aaagacggta ataaactgga tgtctatggc
      121 aaagttaaag ccatgcatta tatgagtgat aacgccagta aagatggcga ccagagttat
      181 atccgttttg gttttaaagg tgaaacacaa attaacgatc aactgactgg ttatggtcgg
      241 tgggaagcgg agtttgccgg aaataaagcg gagagtgata ctgcacagca aaaaacgcgt
      301 ctcgcttttg ccgggttgaa atataaagat ttgggttctt tcgattatgg tcgtaacctg
      361 ggcgcgttgt atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgattcc
      421 tcggcgcaga ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac
      481 accgacttct tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac
      541 gaaaaccgcg acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac
      601 tttggcggca gcgatttcgc cattagtggt gcctatacca actcagatcg caccaacgag
      661 cagaacctgc aaagccgtgg cactggcaag cgtgcagaag catgggcaac aggtctgaaa
      721 tacgatgcca ataatattta tctggcaact ttctattctg aaactcgcaa aatgacgcca
      781 ataactggcg gctttgccaa taagacacag aactttgaag cggtcgctca ataccagttt
      841 gactttggtc tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgagggc
      901 atcggtggtg aagatctggt taattatatc gacgtcggtg ctacgtatta tttcaacaaa
      961 aatatgtcag catttgttga ttataaaatc aaccaactgg atagcg
//
LOCUS       DAZFAH010000468          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFAH010000468 DAZFAH010000000
VERSION     DAZFAH010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.471"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..287)
                     /locus_tag="ACHJWB_005155"
     CDS             complement(90..287)
                     /locus_tag="ACHJWB_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4316580.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(574..>999)
                     /locus_tag="ACHJWB_005156"
     CDS             complement(574..>999)
                     /locus_tag="ACHJWB_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO4316581.1"
                     /translation="KSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFIL
                     ANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYSGGALLVLFATLSG
                     VVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          351 a          253 c          173 g          222 t
ORIGIN      
        1 aaggtaaatt atcatcaaca cataagcaaa ggactgacag gtgccgctaa cacccaccag
       61 ccgcccattt accacaaata aaaaagcctt caggactgaa ggcgtctgta acaaccgcac
      121 tgatagtctg ccagacccgc cataacaagc tgggtcagta ttaactggca gcgttcgcgt
      181 gaaaggtaag tattctgcgc aatctccccg actgtcgccg gttcggtgac gcttaattca
      241 tcaaacacaa ctctggcggt ttctgtcata tcctgctgtt tcagcatgtc tttttaccct
      301 ttccggttaa cgtgacacac caataactct tgccgaaaaa gccagcaagc tgaaagacct
      361 gtattaataa ctaccagcac atttaacgca ctgcgctact ttgcgggcac aaaaaacccg
      421 ctcagaggcg gggtcaagct atgcggcgaa ataaccactc ttaacagcat acctgatttt
      481 ttacgtacgt aaatgctttg ccgtgcatat ttttcatgca aatgtcacgt cctgctattt
      541 ttcagtctta taaatttaaa accatagaaa aaatcaatta tgttttaaaa atggataggt
      601 aaagaataac aagtgacaca gattcaaacc aaaatggaaa agggtggcaa cccacaaacg
      661 cccactccac atccatgaca atccatacac aacaccagat aacgtggcaa ataatacaag
      721 taaagcacca cctgaatagt gataaaaacc aaacaacaaa gccgccacaa ttaatgcaac
      781 caatggagac gttacttctg atagccgtga ttgaatatac cctctaaata atgattcctc
      841 tgccagagac acaaaaaaca aattagccaa tataaactct ggcaaccact caggaaaatg
      901 aatctctggc tttaatccac caaaaaaaac agccaaaaac aggataagag gaacagagag
      961 cgacagagcc ccccacttcc acaaagacac ttctgattt
//
LOCUS       DAZFAH010000469          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFAH010000469 DAZFAH010000000
VERSION     DAZFAH010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.472"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..582
                     /locus_tag="ACHJWB_005157"
     CDS             <1..582
                     /locus_tag="ACHJWB_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316582.1"
                     /translation="GIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSI
                     GSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEA
                     TSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGP
                     QALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            579..908
                     /locus_tag="ACHJWB_005158"
     CDS             579..908
                     /locus_tag="ACHJWB_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316583.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            908..>987
                     /locus_tag="ACHJWB_005159"
     CDS             908..>987
                     /locus_tag="ACHJWB_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4316584.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEI"
BASE COUNT          206 a          237 c          339 g          205 t
ORIGIN      
        1 ggtattgcac agaatatggc ggcgatgctg accggcagtg agcagaactg gcgcagcttc
       61 acccgttccg tgctgtccat gatgacagaa attctgctta agcaggcaat ggtggggatt
      121 gtcgggagta tcggcagcgc cattggcggg gctgttggtg gcggcgcatc cgcgtcaggc
      181 ggtacagcca ttcaggccgc tgcggcgaaa ttccattttg caaccggagg atttacggga
      241 accggcggca aatatgagcc agcggggatt gttcaccgtg gtgaatttgt cttcacgaag
      301 gaggcaacca gccggattgg cgtgggaaat ctctaccggc tgatgcgcgg ctatgccacc
      361 ggcggttatg tcggtacacc gggcagcatg gcggacagcc gctcgcaggc gtccgggacg
      421 tttgagcaga ataaccatgt ggtgattaac aacgacggca cgaacggtca gataggacca
      481 caggcgctga aggcggttta tgacgtagcc cgtaaggcgg caatggatgt tgtgaccggg
      541 cagatgcgcg atggtggtct gttctccgga ggtggacgat gaaaaccttc cgctggaaag
      601 tgaaacccgg tatggatgtg gcttcggccc cttccgtaag aaaggtgcgc tttggtgatg
      661 gctattccca gcgagcgcct gccgggctga atgccgacct gaaaacgtac agcgtgacgc
      721 tttctgttcc ccgttgggag gccacggcgc ttgagtcgtt tctggctgag cacgggggct
      781 ggaaatcctt tctgtggacg ccgccttatg agtggcggca gataaaggtg acctgcgcaa
      841 aatggtcgtc gcgggtcagt atgctgcgtg ttgagttcag cgcagagttt gaacaggtgg
      901 tgaactgatg caggatatcc ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc
      961 ggccagcgtg gtgctctggg aaatcga
//
LOCUS       DAZFAH010000470          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFAH010000470 DAZFAH010000000
VERSION     DAZFAH010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.473"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..158
                     /gene="ymiC"
                     /locus_tag="ACHJWB_005160"
     CDS             63..158
                     /gene="ymiC"
                     /locus_tag="ACHJWB_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4316585.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            322..>980
                     /locus_tag="ACHJWB_005161"
     CDS             322..>980
                     /locus_tag="ACHJWB_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO4316586.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT          240 a          246 c          248 g          246 t
ORIGIN      
        1 accacagcaa taacagcttg agttatctca acacaaaata ataaccgtta agggtgtagc
       61 ctatgatcaa cacaaatatg aaatattggt cctggatggg cgcgttttct ctgtcgatgc
      121 tcttctgggc cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg
      181 gttgtcattt gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat
      241 cgttacgcga tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa
      301 ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg
      361 caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa atcactgggc
      421 gatatcaccc gtctgcccaa gtcactcaaa gttttgctcg aaaacctgct gcgctggcag
      481 gatggtaact cggttaccga agaggatatc cacgcgctgg caggatggct gaaaaatgcc
      541 catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc
      601 gtacctgccg ttgttgatct cgctgcaatg cgcgaagcgg ttaaacgcct cggcggcgat
      661 actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc
      721 gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac
      781 cacgaacgtt atgtgttcct gaaatgggga aagcaggcgt tcagtcggtt tagcgtcgtg
      841 ccgccaggca caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg
      901 agtgaattgc aggacggtga atggattgct tatccggata cactcgttgg tactgactcg
      961 cacaccacta tgatcaacgg
//
LOCUS       DAZFAH010000471          975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFAH010000471 DAZFAH010000000
VERSION     DAZFAH010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.474"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            249..839
                     /locus_tag="ACHJWB_005162"
     CDS             249..839
                     /locus_tag="ACHJWB_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4316587.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          310 a          207 c          203 g          255 t
ORIGIN      
        1 acaacttgaa atctggttaa aataacacgc aacactattc ttcttccttg agtccgcccg
       61 gaactcgaaa aacaaaccga gttaaagcca tttttcacaa aatcgatttt gggtctcacc
      121 aaagttacgg ggttgcatac gcattcgttt attttcgaac gtgtacatac aaatatgcac
      181 aaaaataatc aaaattattt tctgagatgc attatgatat gaacaccaat ttcgtataga
      241 gtctcactat gtctcgaatt tttgcttact gtcggatatc aacgctggat cagaccaccg
      301 aaaatcaacg ccgggaaatc gaaagtgcag gttttaaaat caaacctcag caaataatcg
      361 aagaacacat tagcggctca gcagcaacca gtgagcgtcc tggttttaac cggttgcttg
      421 ctcgcctgaa atgtggtgat caattgattg tgacaaaact ggatcgcctt ggttgtaatg
      481 caatggatat caggaaaaca gtggaacaac tgaccgaaac aggtatcaga gtgcattgct
      541 tagcattggg gggcattgac ctgaccagtc caacaggaaa aatgatgatg cacgtaattt
      601 cagcagtcgc tgaatttgaa cgagaccttt tacttgaacg cactcattcc gggatagtaa
      661 gagcccgcgg cgcagggaaa cgttttggtc gaccacctgt gttaaatgaa gaacagaaac
      721 aggtggtatt cgaacgaatt aagtcaggtg taagtataag tgccattgcc cgggaattca
      781 aaacctcgcg gcaaaccatt ttaagagcca aagcaaaact tcagacacct gacatataaa
      841 aaataatctc ggtgtgagat gctttacgtc ttccaagccc ccttccttgc cgtaaatgga
      901 aagatacatc taattataga atttatatgt tttaccctac ggcagtgctg gccattcaat
      961 atcctgtgca gttga
//
LOCUS       DAZFAH010000472          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFAH010000472 DAZFAH010000000
VERSION     DAZFAH010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.475"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHJWB_005163"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHJWB_005163"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4316588.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          254 a          237 c          209 g          253 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccaa tagttcatcc agtgcttcaa tatcgcgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ttcatgagtt
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgc
      601 cgaccaatga cagccagcaa atcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgccg caacaatcat
      901 gtcttattaa cattctgtta catgcagatt taaagaaaaa tgcgaaacat atc
//
LOCUS       DAZFAH010000473          948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFAH010000473 DAZFAH010000000
VERSION     DAZFAH010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.476"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..427)
                     /locus_tag="ACHJWB_005164"
     CDS             complement(5..427)
                     /locus_tag="ACHJWB_005164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316589.1"
                     /translation="MVEPVGGRTIPYPPSNPGSADTHTLYQNNSNAYRLNPIGHANNS
                     TPHAHAHLPGTGSGMKGQGPSLDVNGRVVPWNSSAAHIGIRSLGTLSFFSSVLHMFTL
                     RSCKEGTFGQCYCAMYVEMSTGSNQDEADIMCGMPEYF"
     gene            complement(542..948)
                     /locus_tag="ACHJWB_005165"
                     /pseudo
     CDS             complement(542..948)
                     /locus_tag="ACHJWB_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          243 a          244 c          202 g          259 t
ORIGIN      
        1 tattctaaaa atactcaggc attccgcaca ttatatcagc ctcatcttga ttgcttcccg
       61 ttgacatttc aacatacata gcacaataac attgtccaaa cgttccctct ttacatgatc
      121 taagtgtaaa catatgtaaa acagaactaa aaaagcttag tgtaccgagt gatctaatac
      181 cgatatgtgc ggcactacta ttccatggta caacgcgacc attaacatca agggatggac
      241 cttggccttt cataccgcta cctgttcctg gtaaatgtgc atgtgcatgt ggtgttgaat
      301 tgtttgcatg ccctattgga ttaagcctat aagcatttga attattctga taaagagtgt
      361 gagtatctgc acttcctgga ttactggggg gatacgggat tgttctgcca cctaccggtt
      421 caaccataat attgccttta gaatcaccta taaacctacc aacaggttgt tgagttatag
      481 gatcaatagt acaggattga cttacaggtt tttgtctggg agaaggtaat gataatagat
      541 ctaatcccaa agggtctata tactgaaccg gattcaacgg atactgataa aaattccatc
      601 cccccttcag tccaatcgga tcctgagtga tatatcgccc ctgcagcggg tcataatagc
      661 ggtggcggtt gtaatacagg ccggactcct catcatactg ctgccccggc aggcggataa
      721 gctgctgcag ctgatgcggg ttctcttcat tcagcaggtt gccccattca tcgtattctg
      781 cgcaccattc tgttgcccct tccgcactga taagcgccag cggcggcttt cctcactcac
      841 ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca ccggcgggaa
      901 caccacactg ccaccgtctt cgccgccgga ctgctgaagg gcatccgc
//
LOCUS       DAZFAH010000474          945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFAH010000474 DAZFAH010000000
VERSION     DAZFAH010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.477"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..779
                     /locus_tag="ACHJWB_005166"
     CDS             162..779
                     /locus_tag="ACHJWB_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4316590.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
BASE COUNT          260 a          204 c          239 g          242 t
ORIGIN      
        1 tgcttaaaat aattttaatt tcactgaaat ttaacaagtg actttcagga aaatgcctcg
       61 cagatgcggg gcatttttgt accggtattt caccgcgcac cgcagcgcac aataaacacc
      121 gaacctgacc ctttggaatg ggcctttgag gataccagtt agtgctggcg agcctcggtg
      181 ggctggtttc ctgtgcggca aaggttcatt tcaaagaagc aggcaacgcc atgaatgaat
      241 taattgcgaa tcatgacttc gactttcgcc agttagttac cgcagcagaa ggtcaaccgg
      301 taactgacac cttccagatt gccagggcat ttggtaaacg ccatcagcat gtgattaggg
      361 ctattaaatg tttgagatgt tctgaggaat tctcgacaac ccatttttgg gccgtcgaga
      421 aaatcaatga cttaggtatt tttgacaaga aacagattta ctaccgcatg gactttagtg
      481 gcttcgttat gctggttatg ggatttaacg gggcaaaagc cgatgctgtt aaagaagcct
      541 atatcaatgc gtttaactgg atgtcagcag aactccgtaa gtacagcgaa agttatgaag
      601 cagaacgtaa cgccgtaatg ctggagtaca tgaaagagaa ggatgtcgcc agcatgtcag
      661 gccgtctgct caatcgctgg gggagaacga aaaaacctca attgcttgca aagctggaac
      721 gtctggagag acagggacag tttttattac cgggattcga taaaggtatt caagcctgac
      781 acattatgcg ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag cccgacgggg
      841 aattccttct gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg gttttactgt
      901 gctgacagac gcagggttac cctcatagtc gcttttccgg tgcga
//
LOCUS       DAZFAH010000475          941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFAH010000475 DAZFAH010000000
VERSION     DAZFAH010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.478"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>941
                     /locus_tag="ACHJWB_005167"
     CDS             <1..>941
                     /locus_tag="ACHJWB_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4316591.1"
                     /translation="ALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVVK
                     PSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRSTV
                     TPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWGVDKPLYKDLIGRTKAALKK
                     NPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLADMAGQCVGGSADGVPWI
                     CGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMT"
BASE COUNT          218 a          225 c          295 g          203 t
ORIGIN      
        1 gcgcttgctc aaaaaattcg tgaaggatgg cagccatacg gcgggccgtt ttcttcgtat
       61 acggatgatg gcgcagcact tattcaggcg attgtcgcag aaggtgatgt gagcacacct
      121 gttgtggtga agccgtcgga tggagagggc acagtaatca gcgccaccag agacccggag
      181 tattactttg ttgtggttct ggcggggcag tcaaacagca tggcatatgg tgaaggcctt
      241 ccgctgccgg agacatatga ccgtccggac ccgcgcatta agcagctggc gcgtcgcagt
      301 acggtgacac cgggcggtgc cgcctgtaag tataacgaca tcattccggc ggaccattgt
      361 ctgcatgatg tgcaggacat gagccgcctt aaccatccga aagcggacct gtcaaagggg
      421 cagtacggta ccgtggggca ggggctgcat attgccaaaa aactgctgcc gtttataccg
      481 gcgaatgcgg gcattctgct ggttccgtgc tgtcgtggtg gttcagcgtt caccaccgga
      541 gccgatggca catacagtga cgctggcggt gcctcggaga attcaacccg ctggggtgtg
      601 gacaagccgc tgtataagga ccttatcggt cgaacaaaag cagcactgaa gaagaatccg
      661 aaaaatgtgc tgtttgccgt ggtgtggatg cagggggaat ttgattttgg cggtacgccg
      721 gtaaatcacg ccgcacagtt tggtgcgctg gttgataaat tccgtgcaga cctggcggat
      781 atggcaggtc agtgcgtcgg tggctctgct gacggtgttc cctggatatg cggggacacg
      841 acgtatttct ggaagcagaa gaacgaatcc tcgtaccaga cggtgtacgg cagctacaaa
      901 aacaaaacgg aaaagaatat ccatttcgta ccgttcatga c
//
LOCUS       DAZFAH010000476          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFAH010000476 DAZFAH010000000
VERSION     DAZFAH010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.479"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHJWB_005168"
     CDS             complement(<1..305)
                     /locus_tag="ACHJWB_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO4316592.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKDVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSL"
     gene            complement(340..591)
                     /locus_tag="ACHJWB_005169"
     CDS             complement(340..591)
                     /locus_tag="ACHJWB_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4316593.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            700..>940
                     /locus_tag="ACHJWB_005170"
     CDS             700..>940
                     /locus_tag="ACHJWB_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316594.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEME"
BASE COUNT          252 a          221 c          193 g          274 t
ORIGIN      
        1 ttgagtgatg ttttggcgac ttcagtttct ttcagtgcca gaaatttttc agccagttct
       61 tttatggtta aatcttgtcg ggcctcacca aatttttcca gattgcgtga ggagggaaac
      121 tgttttgcat agtcgaaaac accagttttt attgcgtaac aaacagagga gcgtagttca
      181 cctgcaacgc gcctgttttt tgctgtgtca ggaaccccca gattttccct gactcttacg
      241 tctttataaa caaaccagat acgtaatttc cctccatggt tttccacgcc tgtcggatat
      301 ttcatttcaa cttctctcat tagttagtgt ggcttttagt caagtaagat gacgtcttgg
      361 tctcgctgat gcctggcgct caatccagcg atcaatttct tccaggttgt aaaagcatgg
      421 actgttatcc catggcatac cgtcatgagc gacatgctta tattcccttc cttccataaa
      481 cgatttttcc cgggcctttt ttaacgtacc tttttttatt cctttcagcg caattaactg
      541 ctcttcggat acccatttgc cgggagagac aatcatgatt acttcgctca tcgatttctt
      601 tatctcttac atcagacgag cgccggttga agtggcacac tgaatttggc cacctgaaca
      661 gaggtgatat gctcacctca gaacaacaca ggtgctccaa tgaaaaaaag aaattttagc
      721 gcagagttta aacgcgaatc cgctcaactg gttgttgacc agaaatacac ggtggcagat
      781 gccgccaaag ctatggatgt tggcctttcc acaatgacaa gatgggtcaa acaactgcgt
      841 gatgagcgtc agggcaaaac accaaaagcc tctccgataa caccagaaca aatcgaaata
      901 cgtaagctga ggaaaaagct acaacgcatt gaaatggaga
//
LOCUS       DAZFAH010000477          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFAH010000477 DAZFAH010000000
VERSION     DAZFAH010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.480"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..>921
                     /locus_tag="ACHJWB_005171"
     CDS             126..>921
                     /locus_tag="ACHJWB_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4316595.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEYYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMHIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDA"
BASE COUNT          270 a          210 c          229 g          212 t
ORIGIN      
        1 aggataataa cgaaatcccg gcaggatttt aaaacagcac cgtaataaat atccagtttc
       61 attcttatat gccagcaatg gcagggattt gttcatcctt aaatctgtca tgaggttaaa
      121 acaaaatgag taaagtcttt atttgcgccg ctattcctga cgaactggca acaagggaag
      181 aaggcgctgt ggctgtagcc acagccattg aagctggcga cgaacgccgt gctcgagcaa
      241 aatttcactg gcaattcctg gaatattatc cggctgctca ggactgcgct tataaattta
      301 ttgtctgcga ggataaacct ggcatacccc gccctgccct cgattcatgg gatgctgaat
      361 atatgcagga aaaccgctgg gatgaggggg ctgcttcctt tgtcccggtt gagactgaat
      421 cagatccgat gaacgtcact tttgacaagc tggcccctga agtacagaac gctgtcatgg
      481 ttaagttcga cacatgtgaa aacatcaccg ttgatatggt tattagcgca caggaattgt
      541 tgcaggaaga catggcaaca ttcgacggac atatcgttga agcgttgatg aaaatgccag
      601 aagttaacgc catgtatccg gagcttaagc tgcatgccat cgggtgggtt aagcataaat
      661 gtaagcctgg tgccaaatgg cccgaaattc aggcagagat gcacatctgg aaaaaacgtc
      721 gcgaaggtga acgcaaggaa accggaaaat acacgtctgt tgttgatctc gcccgcgcca
      781 gaaccaatca acagcacact gaaaattcaa caggaaaaat cagcccggtc attgctgcca
      841 ttcatcgcga atacaagcag acatggaaaa cactggatga cgaactggcc tacgctctct
      901 ggcctggtga tgtggatgcc g
//
LOCUS       DAZFAH010000478          917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFAH010000478 DAZFAH010000000
VERSION     DAZFAH010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.481"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..730
                     /locus_tag="ACHJWB_005172"
     CDS             <1..730
                     /locus_tag="ACHJWB_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4316596.1"
                     /translation="RKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARLVIV
                     NKHYSHRFVNNSYLHLGIFSGRELVGVMQWGYALNPNSGARVVTGTQNREYMELNRLW
                     LHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHE
                     SIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRL
                     NTKLFKVQPYPKPQTVKTGLKESE"
     gene            723..>917
                     /locus_tag="ACHJWB_005173"
     CDS             723..>917
                     /locus_tag="ACHJWB_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316597.1"
                     /translation="MNERLKTHSKTQKKSADLVSNKLRIGVFQRYQMKCKSVSKKSPR
                     YTGNAGPQGPKGDTGAAGPAG"
BASE COUNT          271 a          202 c          228 g          216 t
ORIGIN      
        1 cagaaagcaa gtcacatccc gtatcatcag cacgcctgaa cttattcgct ataacgacaa
       61 tatcgttggt tatggctccc gtgagttgcg ggttgagaca ataagttgct ggctggccag
      121 actggtcatc gtcaataaac attacagcca ccgtttcgta aataactcct accttcatct
      181 gggcatattt tcaggacggg aacttgttgg cgtaatgcaa tggggttacg cccttaaccc
      241 aaacagtggt gcacgcgtcg taacgggtac gcagaaccga gaatatatgg agcttaaccg
      301 gctatggctg catgactgta tgccgagaaa ctccgaatca agggcaatca gttacgcgct
      361 caagctaatc aggcagcttt atccgcaggt acagtgggtt cagtcgtttg cagatgagcg
      421 ttgtggctgt cttggtgtcg tgtatcaggc aagcaatttt gattatgtag gcagccatga
      481 atcaatattc tatgaacttg atggcgagtg gtatcacgag atttgcagga atgccattaa
      541 acgaggcgga caacgaggtg aacatctgag ggctaatatt gacagagcca gtgttcataa
      601 gttccgacag tttcgttaca tccggtttct gaacaagagg gccagaaaac gcctcaacac
      661 aaagctattc aaagtccagc cttacccaaa accacaaaca gttaaaactg gtttgaaaga
      721 gagtgaatga gcgcttaaaa actcattcta aaactcaaaa gaaaagcgcc gatttagtat
      781 caaataagtt gcgtatcggc gtttttcagc ggtatcaaat gaaatgcaaa tcggtatcaa
      841 aaaaatcgcc gcgctatacg ggaaatgcag gtccacaggg tccaaaggga gataccggtg
      901 cggcaggccc ggcaggc
//
LOCUS       DAZFAH010000479          913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFAH010000479 DAZFAH010000000
VERSION     DAZFAH010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.482"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>913
                     /locus_tag="ACHJWB_005174"
     CDS             <1..>913
                     /locus_tag="ACHJWB_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4316598.1"
                     /translation="GISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDV"
BASE COUNT          205 a          224 c          302 g          182 t
ORIGIN      
        1 ccgggatatc agtcggacag tataaggctg ccatgcgcac actgcccgca cagttcacgg
       61 atattgtcac tcagcttgcc ggtggtcaga atcccttcct tatcatgctg cagcaggggg
      121 ggcagatcag cgattcattc ggtggaccgc tcagcctgct taccctgctg aaggaggaac
      181 ttctcgggat cagggatgcc tctgaatcat cagaggagtc gctgtcagat acggcaaatg
      241 cactggctga aaatgcccgg aatgccggtg agctgggacg atttatgtcg gtggcccgtg
      301 tggcggcagg tggcggggtt gccgtactgg ccgcgcttgc tgccgccgcc tggcaggcag
      361 agcaggctga ccgggcctta ttgcgttcac tgatcctgac cggaggggcg gctgccacca
      421 caacggcaga attgtggaaa atggccgggg tgatcagcga tgaagccggt ggtggtatca
      481 gacaggcggc agaaaatctg gcccgtctgg cagaaagcgg gaaatatacc gccgggcagc
      541 tacggatcat gggggaaacc tctcagagat ggctgcagac ggtgggggac gatgccggga
      601 aggtggaaaa agcctttgaa gggattgcag cagatccggt gaaggcgctg gcctccctga
      661 atcagcagta taacttcctg agcgtttccc agttacgcca tattgatgag cttgagcgca
      721 cgaaaggtaa acaggctgcg gtgacggagg cgatgtccct gtttgcggat gtcatgaatg
      781 cacgtctgga gcaacttgat aaagcggcca cgccggtgga aaaaatctgg gacgatgtta
      841 aaacctggac ttctgacgca tgggcatgga taggtgatca tacactgggg gcactcagtc
      901 tgatcactga cgt
//
LOCUS       DAZFAH010000480          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFAH010000480 DAZFAH010000000
VERSION     DAZFAH010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.483"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..207
                     /locus_tag="ACHJWB_005175"
                     /pseudo
     CDS             97..207
                     /locus_tag="ACHJWB_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(394..594)
                     /locus_tag="ACHJWB_005176"
     CDS             complement(394..594)
                     /locus_tag="ACHJWB_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316599.1"
                     /translation="MEKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(669..>905)
                     /locus_tag="ACHJWB_005177"
     CDS             complement(669..>905)
                     /locus_tag="ACHJWB_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4316600.1"
                     /translation="IKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDEVLA
                     RMVGHSPAENSTENEPQPVEQPEPVQPGTDSAMM"
BASE COUNT          227 a          220 c          197 g          261 t
ORIGIN      
        1 taaaagggaa acccagcata gctggaacta tgctgggccg atttagacaa gtttgcatca
       61 atgctttact tcgggactac ataaatggcg actcagatag cgcgaactat tctgggtcaa
      121 tctgtaaaaa cttgcatcaa tgcttcactc tgagctcgtg gtgtatccta tgatattccc
      181 cggtctcctg taaagcagag atgcatctgt tcaggcagga gaaaatggat aaactgacca
      241 ccgaccaaaa gttacaggct gcgcgaattg cagctgatat cacaattgca gcaatgacat
      301 ccaccagttg tgtctttacc tcttttcata cagccggtga ttcatcagct atgcaattat
      361 ggaacgcgtg cttttctgat gtaattcggg caatcagcga gtcaactgac cctcacgaac
      421 cgcaaaaata gcgcatcttg gggtacacag gatctgcatc gctatccaca ttgcagtggt
      481 gaagtttcta tcctgtaatg tattttcatc aagtcctgaa cgtagcgcat tgataatttc
      541 tgcgcttaat atttccagac ctttggtatc gggctgcttt ttgttcttct ccatgattat
      601 ttcctctgac tggtatatac atcgctcatg aaaatcaaac cgacaggaga agctgtcggt
      661 ttgtattgtt acatcatggc actgtcagtt ccgggctgaa ccggctctgg ttgctctacg
      721 ggctgaggct cattctccgt gctgttttca gccggagaat ggccaaccat tcgggccagc
      781 acttcatcaa catatgcgtc gatttgtgcc tcaaaatcct tgcgaacctg cgccttcagc
      841 acgcggttca cttcttcaga ataaagtgcc tgtttgacaa gctcttcagt aaccgtgcct
      901 ttaat
//
LOCUS       DAZFAH010000481          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFAH010000481 DAZFAH010000000
VERSION     DAZFAH010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.484"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>900
                     /locus_tag="ACHJWB_005178"
     rRNA            <1..>900
                     /locus_tag="ACHJWB_005178"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          222 a          207 c          285 g          186 t
ORIGIN      
        1 tctgatactg gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa
       61 atgcgtagag atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg
      121 ctcaggtgcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa
      181 acgatgtcga cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt
      241 cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca
      301 caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac
      361 atccacggaa gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca
      421 tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct
      481 tatcctttgt tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg
      541 aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct
      601 acaatggcgc atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt
      661 cgtagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg
      721 gatcagaatg ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg
      781 ggagtgggtt gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg
      841 attcatgact ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac
//
LOCUS       DAZFAH010000482          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFAH010000482 DAZFAH010000000
VERSION     DAZFAH010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.485"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..548
                     /locus_tag="ACHJWB_005179"
     CDS             15..548
                     /locus_tag="ACHJWB_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316601.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESVLACWGIDR"
     gene            819..>894
                     /locus_tag="ACHJWB_005180"
     CDS             819..>894
                     /locus_tag="ACHJWB_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316602.1"
                     /translation="MNMMTVPFHGNSLYVVNHNGEPYVP"
BASE COUNT          235 a          201 c          243 g          215 t
ORIGIN      
        1 cctgataaat atccatgaac gcaaaaatca gatacggcct gtcggctgcc gttctggcgc
       61 tgattggtgc aggggcgtct gcgcctgaaa tcctcgacca gtttctggat gaaaaggaag
      121 gtaaccacac cacggcatac cgtgatggtg cgggtatctg gaccatctgc cgaggtgcca
      181 tcatggtgga tggtaagcct gtgattcctg gcatgaagct gtcgaaggaa aaatgcgacc
      241 aggttaacgc cattgagcgt gataaggcgc tggcatgggt ggagaaaaac atcaaagtgc
      301 cgctgaccga accccagaaa gcggggatcg cgtcattctg tccgtacaac attggtcccg
      361 gtaagtgttt cccgtctacg ttttacagac gaattaatgc aggtgatcga aaaggtgcct
      421 gcgaggcgat tcgctggtgg attaaggacg gtggcagaga ctgccgtatt cgttcaaaca
      481 actgttacgg tcaggtatcc cgtcgtgacc aggagagcgt gctggcgtgc tggggaatcg
      541 acagataagc agaatatttt gctgaaaaat gaggtttgct tacatggatg tataacacga
      601 aatcctgcaa attggcaaaa tgtaagtgaa taaagtcaaa acagttgttt aacactcagg
      661 caccgtaatg gtgcctttgt catttctgcg catctcacgc gcatctcaca acacagaacc
      721 tttcaggatg acccttgagg ataccggttt ggctgtcggt gcctttctgt gggctggatt
      781 cctgtgagac aaggttcatc actaaaagga aataaccgat gaatatgatg accgtgccgt
      841 ttcacggcaa ctctctttat gtagttaacc ataatggcga gccatacgtt ccca
//
LOCUS       DAZFAH010000483          893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFAH010000483 DAZFAH010000000
VERSION     DAZFAH010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.486"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..451)
                     /locus_tag="ACHJWB_005181"
     CDS             complement(<1..451)
                     /locus_tag="ACHJWB_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4316603.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMIVLHDSYDN"
     gene            complement(458..>893)
                     /locus_tag="ACHJWB_005182"
     CDS             complement(458..>893)
                     /locus_tag="ACHJWB_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316604.1"
                     /translation="EQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRAT
                     TFFGPDRNFENSWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT          165 a          287 c          219 g          222 t
ORIGIN      
        1 cgttgtcgta gctgtcatgc aacacgatca tgacatcaga cacggtgacg ataatcacgc
       61 tgcgcccctt cgccatcagc cggttaccca tcgccgctgc aaggtgattt ttcccggtgc
      121 cggttttacc gctgaacaca aaattcgtgc acccggtcat cagttcgtca gctatggatt
      181 tggcctggct cagcgcgtat ttttgcccgt cgttctgcac ctgataattc gcaaacgagc
      241 atttgctgtg cagaggctgg atgcccgaac gattcaggat tttttccacc cgcaactggc
      301 gattctggcg gttaatctcc tcgctgcgtt ttcgtccttc agcaagttgc cattcccgcc
      361 actcctccac cgtccggtac ggtggaaccg ccccctgtgg tgcaagtctg cgaatacgtt
      421 caagaacccc aactgccgca atgtttttca tgacacgtca ccccctgaat cccggcggta
      481 tttcagtgtc cggttcagaa atgtgattca cgcaacgctg gttgttcgtg ccgcttaccg
      541 ggagcaacca ggagttttca aaattccggt ccggcccaaa aaacgtcgtc gctcgctgaa
      601 caaattccgt tcccgctttc ccggtcgccg ccaggtatct cgcgtaacgc ctcacaccat
      661 ccagcatggt ctctggtggc accccctcgc gcaatctggc cttccaggca ctgaatgcgg
      721 atttcttcgg gtttgctccg gcacgcaacg ggtactcccg ccagacctgt tcgaacacat
      781 ccggataatc cactcgtccc acagactgcc cggtgttttc cgggactacc cgatcggctt
      841 cccgctgaat ggcggaatcg gcttcgggct gctgcagttg gtgtgattgc tcc
//
LOCUS       DAZFAH010000484          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFAH010000484 DAZFAH010000000
VERSION     DAZFAH010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.487"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>871
                     /locus_tag="ACHJWB_005183"
     CDS             <1..>871
                     /locus_tag="ACHJWB_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316605.1"
                     /translation="ERFTIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARV
                     RLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYK
                     NARQEHTSLPNLLERQFAVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSL
                     SAHADTALISSALRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRG
                     NCWDNSPMERFFRSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPE
                     ESENRYHFYCKTVA"
BASE COUNT          223 a          222 c          247 g          179 t
ORIGIN      
        1 ctgaacggtt cacgatagcc gccagactaa gtgacagcca cacggttgtc agcctgtgtt
       61 ctgctctgga aatacaccgc agcagttacc ggtactggcg aaaacgacgc gatacggtta
      121 atccggcacg agtcaggttg tgcagcgaaa tacgccgggc gtggaaccaa agtcggggct
      181 ctgcgggcgc gcgcactctg gctgaaatgc tgactcaaaa cggcgtcccg atgagccgtt
      241 accgtgccgg acgtctgatg aaatatctga acctgagcag ttgtcagccc ggaaaacatc
      301 agtataaaaa tgcccgtcag gagcatacca gcctgccgaa tctgcttgag cgtcagttcg
      361 cagtaccgga gccagaccgg gtatggtgtg gagatattac gtatctctgg gcaggaaatc
      421 gctggtgcta tctggcggtt gttatggatc tttttgcccg cagggttatc ggctggagtc
      481 tgtcagcgca tgccgatacc gcactgataa gcagtgccct gcggatggcc tatgagacgc
      541 gtggccaacc ccgggatgtc gtgttccata gcgaccaggg aagccagtat acaggcctta
      601 aatatcaaca acttctctgg cgttgcagaa taaatcaaag cgtcagtcgg cggggaaact
      661 gctgggataa cagccccatg gaacgcttct tccgcagtct gaaaacagaa tgggtgccaa
      721 cgaatggtta cgcaggcaag gacgaggccc ggcagcaaat taatgattac atattgaact
      781 actacaacag cgtcagacct caccattata acggtgggct gacgccggaa gagtcagaga
      841 acagatacca tttttactgt aaaaccgtgg c
//
LOCUS       DAZFAH010000485          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFAH010000485 DAZFAH010000000
VERSION     DAZFAH010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.488"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>869)
                     /gene="escV"
                     /locus_tag="ACHJWB_005184"
     CDS             complement(<1..>869)
                     /gene="escV"
                     /locus_tag="ACHJWB_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4316606.1"
                     /translation="PLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIYSFGN
                     FVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGDMRAG
                     VIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQFD
                     MPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIVQQVS
                     VNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAMGA
                     DLSNSQNISPGAE"
BASE COUNT          288 a          219 c          168 g          194 t
ORIGIN      
        1 ctcagcgccc ggtgagatgt tttggctatt agacaaatca gctcccatag catccttatt
       61 attgccagtt ccaaatgttc ttttcttctg taatttccag gctatcccca acaggcaaac
      121 cgccagaaat aagaagacca atgtaggaaa gcccggaata atcgccatta cgagcattag
      181 tgcggcactg attaaaaaag ggcgactatt aacagaaacc tgttgaacaa tatcacctgc
      241 aaggttttct tctttttcgc tttcaccggg cacacgagta acaaccacgc ccgcggtgac
      301 agaaataata agcgcaggga tctgggcaac caaagcatca ccgacagaca atacagaaaa
      361 caggctaagt gcctcactaa atggcatgtc aaattgccac ataccaatga gcacgccacc
      421 aaaaaggtta actaaaacaa taatgatacc agcaatagcg tcccctttta caaacttcat
      481 cgccccgtcc attgaaccat aaaactggct ttccttttgc actcgggagc gaagaacttt
      541 tgcctcaagc gggtcaataa cgcctgcgcg catatcacca tcaatactca tttgctttcc
      601 tggcatacca tcaagggaaa aacgagcact cacttcagca acacgctcgg ccccttttgt
      661 aataaccata aattgaacaa tggtgataat agtaaaaata accaggccaa caacaatatt
      721 accgccaaca acaaagttac cgaaggagta aataatgtca cccgcatcgt gatgcagcag
      781 aataagtctg gttgtactaa cactgagcga caggcgcatc aacgtcgtaa tcaacaggat
      841 tgtggggaaa gaagttagtt caagaggat
//
LOCUS       DAZFAH010000486          862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFAH010000486 DAZFAH010000000
VERSION     DAZFAH010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.489"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..473
                     /locus_tag="ACHJWB_005185"
     CDS             <1..473
                     /locus_tag="ACHJWB_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316607.1"
                     /translation="VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIAS
                     AVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEI
                     IFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            549..>862
                     /locus_tag="ACHJWB_005186"
     CDS             549..>862
                     /locus_tag="ACHJWB_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO4316608.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TE"
BASE COUNT          216 a          207 c          233 g          206 t
ORIGIN      
        1 ccgtggcatc ttccggtgtg gcaggtggag ctgtcgggtc agtggcgggc gggatactgt
       61 tcagtccact gggaccattt ggtgcactga cagggcgtgt ggttggcggt gttctggggg
      121 gaatgattgc ctccgctgta tcaggaaaaa ttgccggagc gctggttgaa gaagatcgcg
      181 tcaaaattct ggcaatgatt caggagcagg tgacatggct tgccggcagt ttcctgctga
      241 ccggacatga gattgaaaat ctgaacgaga atctggcccg tgttatcgat cagaatgctc
      301 tggagatcat tttcgccgcc ggtatacaac aacgggccgc gaccaatatg ttaatcaaac
      361 cactggtggt cagtatcatc aggcaacgcc ccgtcatgga atatgatgca tcccatctcg
      421 gcaatatggt taaccgactg gaagaagcat tacccccgga gttaccggca taacggctat
      481 tctgcgcctt ttacgcttta tcacctgatt tgtcagtgtc atcgtcatga ctcagagagg
      541 taaataccat gattcacctg ttcaaaacct gcatgattac cgccttcatt ctggggttaa
      601 cgtggtctgc cccactccgg gcacaggatc aacgttacat cagtatacgc aatacagata
      661 cgatatggct cccgggaaat atttgtgctt accagttccg gctggataat ggcggaaacg
      721 atgaaggatt tggccccctc accatcactc tgcaactcaa agacaaatat ggtcagacgc
      781 tggtgaccag aaaaatggaa acggaagctt ttggtgacag taatgccacg cgaaccacag
      841 acgcatttct ggaaacggag tg
//
LOCUS       DAZFAH010000487          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFAH010000487 DAZFAH010000000
VERSION     DAZFAH010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.490"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..715
                     /gene="ydeO"
                     /locus_tag="ACHJWB_005187"
     CDS             <1..715
                     /gene="ydeO"
                     /locus_tag="ACHJWB_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4316609.1"
                     /translation="NILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
                     YLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIF
                     AACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQT
                     TFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEY
                     RCQRHTGMNTGNTMSALAI"
BASE COUNT          267 a          167 c          170 g          256 t
ORIGIN      
        1 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
       61 tgttcgtacg cattttgaac cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa
      121 taccattaaa aaatatctac aatgcactaa catccagaca gtgacgatgc ctgttcctgc
      181 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
      241 caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc
      301 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
      361 cgggaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
      421 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaacttaagc aagagcaaac
      481 gacattctca cagattcttt tagatgcaag aatgcagcac gcaaaaaatt tgatacgcgt
      541 agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat
      601 ttatgcgttc cgtaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
      661 tcaaaggcac acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt
      721 tgctaacgag tagtcaacca cgcacgctac gtaaaaatta aaatggggca gtcattccct
      781 gccccgcgtt gtttttaggc gatatattta ttgcaataaa taagtgacat ccatcacata
      841 tttatgcact tggataacct
//
LOCUS       DAZFAH010000488          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFAH010000488 DAZFAH010000000
VERSION     DAZFAH010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.491"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..779)
                     /locus_tag="ACHJWB_005188"
                     /pseudo
     CDS             complement(43..779)
                     /locus_tag="ACHJWB_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          218 a          185 c          198 g          255 t
ORIGIN      
        1 ttattttact tttcccttat cgctgtcgat tctcattatc attcatggca aaaaaacagc
       61 aaaagaggcc ctggtaatga ctcctgtcag tagtacatgt gttttcgata aatgaaccaa
      121 tggcttttcg tgaatttcca gggagcgtaa aaagcaaatc gttttccggg caggtcgttt
      181 gatgtgagta cgaagtgtca gattgttgcg ctcaatgcgc cgggtaaata tcttgccgac
      241 aagatgctta tcctgtggca tttctctggt ataactgctc cggttgtctc tggttatcat
      301 gcctgcggag aatggcttca ggaattccgg caactcacgg catgtttcat cagtgcgagg
      361 accaaaagtg taagccagca caccgtcagc tttggtctta tacgcgtacc agtgccactg
      421 ctgacgagct ttgttttcga caaaactcca ttgttcatca agttcacaga taagtgccac
      481 atcgacactg gcggctggag atatggttac tttacgtggt gagcgttttt taaagtgaga
      541 atgacggtgt tgatatccac cttcagcgtt ctggagctat cgcgaacccc ggctccgtta
      601 tgaaccattt caacaatttg ctctttaatg cctggttttc gggtttcgta gatgtaattc
      661 agttgaaata cacgtttgca ggattagcac tggaaacgct catgatctga ggtgctgtga
      721 tcataacggt acactttata ggaattacag cggggagaat atactgtaac tgttgccata
      781 tggtctccag ataccaatag aatacaacat taatctatcg tcagaaggca tcaccggggc
      841 cttctgacat aatctg
//
LOCUS       DAZFAH010000489          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFAH010000489 DAZFAH010000000
VERSION     DAZFAH010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.492"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..819
                     /gene="fimB"
                     /locus_tag="ACHJWB_005189"
     CDS             217..819
                     /gene="fimB"
                     /locus_tag="ACHJWB_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4316610.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          249 a          161 c          192 g          252 t
ORIGIN      
        1 tataaaggta catagcatgc aagagcatgg cgtttgtatg gcaacgttat tataattaac
       61 tgttgctact ccatttaagt tcactcagaa gaactggtcc acttacgtta gttattaagc
      121 aaacgttcgc ttttataaac ataatcagga taaaaatgtt ggattattgc taacccagca
      181 cagctagtgc gcgtctgtaa ttataaggga aaaacgatga agaataaggc tgataacaaa
      241 aaaaggaact tcctgaccca tagtgaaatc gaatcactcc ttaaagcagc aaataccggg
      301 cctcatgcag cacgtaatta ttgtctgact ttgctttgtt ttattcatgg tttccgggcg
      361 agtgaaattt gtcgattgag gatttcggat attgatctta aggcaaagtg tatatatatc
      421 catcgattaa aaaaaggctt ttcaacaacg catccgctat tgaacaaaga agttcaggct
      481 ttaaaaaact ggttgagtat ccgtacttcg tacccgcatg ctgagagcga gtgggtattt
      541 ttatctcgta agggaaatcc gctttctcgg caacagtttt accatattat ctcgacttcc
      601 ggtggtaatg ccgggttgtc actggagatt catccgcaca tgttacgcca ttcgtgtggt
      661 tttgctttgg cgaatatggg aatagatacg cgacttatcc aggattatct tgggcatcgc
      721 aatattcgtc atactgtctg gtataccgcc agcaatgcag ggcgttttta cggcatctgg
      781 gatagagcca gaggacgaca gcgtcacgct gttttataga gatagttgag ataccaggga
      841 tggtgttaaa aact
//
LOCUS       DAZFAH010000490          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFAH010000490 DAZFAH010000000
VERSION     DAZFAH010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.493"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..805
                     /gene="nfeF"
                     /locus_tag="ACHJWB_005190"
                     /gene_synonym="yqjH"
     CDS             41..805
                     /gene="nfeF"
                     /locus_tag="ACHJWB_005190"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4316611.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT          193 a          224 c          240 g          194 t
ORIGIN      
        1 tatttagata tatctgatta ctatcacgaa ggcgataaca atgaataact ccccccgcta
       61 cccgcagcgc gttcgcaatg atctgcgctt ccgtgaactg actgtgttac gcgttgagcg
      121 catcagcgcc ggttttcagc gcattgtcct cggcggcgag gcgctggacg gttttacatc
      181 gcgcggcttt gacgatcaca gcaaactctt ctttcctcaa cctgacgctc actttgtgcc
      241 gccaacggta acggaagagg gcatcgtctg gccggaagga ccacgcccac cgtcgcgtga
      301 ctatacgccg ctgtatgacg aactacgcca tgaactggcg atcgatttct ttattcacga
      361 cggtggggtc gccagcggct gggcgatgca ggcgcaaccg ggcgataaac ttacggtggc
      421 aggtccgcgc ggttcgctgg tggtgccgga agattacgcg tatcagctgt atgtctgcga
      481 tgaatccgga atgcccgcac tgcgccgccg cctggaaacg ttgagcaaac ttgccgttaa
      541 accgcaagtt agtgcgctgg ttagcgtgcg ggataacgcc tgtcaggatt atctcgcgca
      601 ccttgatggt tttaatatcg aatggctggc acatgatgag caggcggtag atgcgcgtct
      661 ggcgcagatg caaatccctg ccgatgatta cttcatctgg ataaccggcg aaggtaaagt
      721 cgttaagaat ttaagccgcc gctttgaagc ggaacagtat gacccacagc gggtacgtgc
      781 agcggcttac tggcacgcaa agtaactgat atataaaaag aaaaaggctg acgatttctc
      841 gtcagccttt g
//
LOCUS       DAZFAH010000491          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFAH010000491 DAZFAH010000000
VERSION     DAZFAH010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.494"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>845)
                     /locus_tag="ACHJWB_005191"
     CDS             complement(<1..>845)
                     /locus_tag="ACHJWB_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316612.1"
                     /translation="NQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAF
                     EYIAGAPQEQKDNPLINILEKFSSWYDTNSVTLGGVKIPHLFPGDDLKLQTAQDSDNG
                     FSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLA
                     TQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMR
                     IEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRP
                     EEETTP"
BASE COUNT          188 a          262 c          193 g          202 t
ORIGIN      
        1 ggtgttgtct cctcttccgg cctgcgactc tccgctatct gctgctgata cgcctgcgct
       61 atccacaccg gacgtgagag tccggctttt tcccgctctg cagattccct gacctgctgg
      121 cggaaaatgt cctgataatc ctcacccatc agcgccagct ctttctcata cgtgctcagt
      181 ccggcctcaa tgcgcatcac tgattcctgc acctccttga gtccgtcaat ggccattctt
      241 ccggctccaa tccactctgc ccgtgaccag gctgaacgcg cctgataaaa atcaaaacgc
      301 gcccgtggtg gacgaataat cccccgcaga agagcctctt ccagccagca ggaaaacatc
      361 tgcgtggcca gccgggccgc aataaatttt cgccgcccca taaaatagcg ccacgactca
      421 ttggcagagg ccctggcact tgaataactg accttcgagt aatcacggga caactgttcg
      481 taggaaacgc caagaccggc ggcgatatac cgcagcagcg cctgttcaag cgccgaaaat
      541 ccattgtctg aatcctgcgc agtctgtagt ttcagatcat caccagggaa aaggtgcgga
      601 attttgactc cgcccagcgt cacgctattc gtgtcatacc agctggagaa cttctccaga
      661 atattaataa gcggattatc cttctgctcc tgtggcgcgc cggcgatata ttcaaaggct
      721 ttttcggtat caagttcact ttcaatcgtc gctgcataca tcgccttcac tatggccgac
      781 tgaagctgtg ttgcctgcag ggaatccagc atcttcagcc gttccatgac gctgtaaaac
      841 tgatt
//
LOCUS       DAZFAH010000492          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFAH010000492 DAZFAH010000000
VERSION     DAZFAH010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.495"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>845)
                     /locus_tag="ACHJWB_005192"
     CDS             complement(<1..>845)
                     /locus_tag="ACHJWB_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316613.1"
                     /translation="NQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESDLDTEKAF
                     EYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGDDLKLQTAQDSDNG
                     FSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLA
                     TQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMR
                     IEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYQQQIAESRRP
                     EEETTP"
BASE COUNT          189 a          261 c          201 g          194 t
ORIGIN      
        1 ggtgttgtct cctcttccgg cctgcgactc tccgctatct gctgctgata cgcctgcgct
       61 atccacaccg gacgtgagag tccggctttt tgccgctctg cagattccct gacctgctgg
      121 cggaaaatgt cctgataatc ctcgcccatc agcgccagct ctttctcata cgtgctcagt
      181 ccggcctcaa tgcgcatcac cgattcctga acctccttga gcccgtcaat ggccattctt
      241 ccggcaccaa tccactctgc ccgtgaccag gctgaacgcg cctgataaaa atcaaaacgc
      301 gcccgtggcg gacggataat cccccgaaga agtgcctctt ccagccagca ggaaaacatc
      361 tgcgtggcca gccgggccgc aataaatttt cgccgcccca taaaatagcg ccacgactca
      421 ttggcagagg cgcgggcact tgaataactg accttcgagt aatcacggga caactgttcg
      481 taggaaacgc caagaccggc ggcgatatac cgcagcagcg cctgttcaag cgccgaaaat
      541 ccattgtctg aatcctgcgc agtctgtagt ttcagatcat ccccggggaa aaggtgcgga
      601 attttgacac cacccagcgt cacgttattc gtgtcatacc agctggagaa cttctccaga
      661 atattaataa gcggattatc cttctgcccc tgcggcgcac cggcgatata ttcaaaggcc
      721 ttttcggtat caaggtcact ttcaatcgtc gctgcataca tggctttcac aatggccgac
      781 tgaagctgtg ttgcctgcag ggaatcgagc atcttcagcc gttccatgac gctgtaaaac
      841 tggtt
//
LOCUS       DAZFAH010000493          844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFAH010000493 DAZFAH010000000
VERSION     DAZFAH010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.496"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..>844
                     /locus_tag="ACHJWB_005193"
     CDS             183..>844
                     /locus_tag="ACHJWB_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO4316614.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDR"
BASE COUNT          211 a          212 c          231 g          190 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg
      601 cgctgacaga tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga
      661 gaccgagcat tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc
      721 agataaaaga cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa
      781 gcatcccggg tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg
      841 accg
//
LOCUS       DAZFAH010000494          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFAH010000494 DAZFAH010000000
VERSION     DAZFAH010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.497"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..756)
                     /gene="lpfA-O113"
                     /locus_tag="ACHJWB_005194"
     CDS             complement(184..756)
                     /gene="lpfA-O113"
                     /locus_tag="ACHJWB_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO4316615.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          244 a          201 c          168 g          228 t
ORIGIN      
        1 cccgcgtacc accaatgaca acgcctgcat aagttaatgc tggttggcct aaaaaaacgc
       61 tgagtataat cgcggtaaat aacttacatg aaactgttgt tttaaagagc ctgattaaac
      121 aactttttct ggacataacg actccctggc caaagccaac tgaataaaaa ggcccttgat
      181 aaattatttc ttatattcga cagtaaattg tgaatccgcg ttcgccgtac cagccgttac
      241 ggtcgcattg gttgccacat agcgtgcaat aaacttcatt tcagccttgc cttcagcatc
      301 gatagcggta taagcagagt cgttataaag tttaacctgc gaattatcgg actggttaaa
      361 aatacgaata ccaacgccgg ttgccgcaat cgcttgtcct gtaccggtaa aatcaccagg
      421 gttgcctgct gtaagcggcg tacccacttt caggtcgccg tcgccgccag gcgcttctgt
      481 accatcaaaa cgaacggctg cttgagtgta cgttgccggg caattttcaa gtttaatact
      541 gaacttctgt ggcgacgcca gactacctac acctgaaaat gcatttttac tgactttacc
      601 cagtgggaca atttgatcgg ctgtatcgga ggtaacttca caagtagtgt cgacaatttc
      661 accgacgaag tgaacaacac catcttctgc cagtgcatgc ccggccagca ttagagatgc
      721 cagagtgaat gcaccgccta taatattacg cttcataaaa actccattga tataatatat
      781 cctttaccat gaatcatcac ttatattaat aagtgtattt ttgattcaaa ggaatagtac
      841 a
//
LOCUS       DAZFAH010000495          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFAH010000495 DAZFAH010000000
VERSION     DAZFAH010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.498"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..207)
                     /locus_tag="ACHJWB_005195"
                     /pseudo
     CDS             complement(1..207)
                     /locus_tag="ACHJWB_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            complement(281..346)
                     /locus_tag="ACHJWB_005196"
                     /pseudo
     CDS             complement(281..346)
                     /locus_tag="ACHJWB_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(348..617)
                     /locus_tag="ACHJWB_005197"
     CDS             complement(348..617)
                     /locus_tag="ACHJWB_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4316616.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGVWENIGGMK"
     gene            complement(619..>836)
                     /locus_tag="ACHJWB_005198"
     CDS             complement(619..>836)
                     /locus_tag="ACHJWB_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4316617.1"
                     /translation="LGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGFFQHL
                     QVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          201 a          228 c          161 g          246 t
ORIGIN      
        1 ccgttgtgac tcttgaaaca ttttggccaa gaatattaag agtatagtca ttaacggatt
       61 tcattcctaa aaaaaaacaa gtaatccttc tttctcttgc gctttttcga ttgttctccc
      121 tgctaaagtt gttgggatgg attgagattg ttttgtcgga actgatacgc tggcgctaat
      181 aggtagtagg gttcttatgc taaacataca acctctttct taattttcga acctgtttag
      241 gattctgtgt aaattcaaaa taaacctgtc tgaacattcc taacaaacat ccaccggaca
      301 taacaacaaa aaccggagcc ggactccggt tttgtgaagc tgtcgggtta cttcatcccg
      361 ccaatatttt cccacacccc gtcagcacgc aggatttgca acggtcttac cacgcactgt
      421 atctgctttt tatccgcatc cagtatcacc acctgcgtga ttaccctgtc ctgctccggg
      481 ataatgccat tctcatcgga ctccagaatg tctgccggtc ccagtcgcag ctgtgctgta
      541 agtaactccc cgttttcacg gtcatcatgc tttccgcaac cacacagacg ctgcataagt
      601 ttttttagta tattcatgtc attctcctgt tctgcctgta tcactgccca cttcatccag
      661 ccccttgaca tcctgccacg gcccgtcacc aaacctgacc tgcaaatgct gaaaaaaccc
      721 ctgaacccgt gtggcatctt tggggtcaag aaaggtcagt ccggtgatga gtgcgccatc
      781 tgtatccggg aaccagccat tgctgtttgt ctcaataatg tttcccggcc ccagac
//
LOCUS       DAZFAH010000496          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFAH010000496 DAZFAH010000000
VERSION     DAZFAH010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.499"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>819
                     /locus_tag="ACHJWB_005199"
     rRNA            <1..>819
                     /locus_tag="ACHJWB_005199"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          227 a          179 c          254 g          159 t
ORIGIN      
        1 gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc aaggctaaat
       61 actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag aaccccggcg
      121 aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca cagagcaatc
      181 tgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa
      241 ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag
      301 acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg
      361 accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa
      421 tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca
      481 tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca
      541 aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg
      601 tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa
      661 cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa
      721 agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg
      781 caccgaagct gcggcagcga cgcttatgcg ttgttgggt
//
LOCUS       DAZFAH010000497          817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFAH010000497 DAZFAH010000000
VERSION     DAZFAH010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.500"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..>817
                     /locus_tag="ACHJWB_005200"
     CDS             16..>817
                     /locus_tag="ACHJWB_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4316618.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLE"
BASE COUNT          259 a          179 c          147 g          232 t
ORIGIN      
        1 taaccggagt agtgtatggc caatctctac gacttgaaaa agttcgactt aaaccttctt
       61 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
      121 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
      181 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac
      241 catcttgaga aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa
      301 ctaaagaaaa actttattat ctacggccct cagctcattt cttgctctaa taatagtatg
      361 ctgatccgct gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg
      421 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
      481 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta
      541 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
      601 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      661 gacgagaagt ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaatcatt
      721 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taattcctac
      781 cgtgactttc ttaatttaaa agagataaaa ctagaac
//
LOCUS       DAZFAH010000498          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFAH010000498 DAZFAH010000000
VERSION     DAZFAH010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.501"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHJWB_005201"
     CDS             <1..186
                     /locus_tag="ACHJWB_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316619.1"
                     /translation="QACADMKRDKNIASVFATGLNEWKQVKAAHVSKIRTLVNEANML
                     IDFVLADTGKGKITKAD"
     gene            186..332
                     /locus_tag="ACHJWB_005202"
     CDS             186..332
                     /locus_tag="ACHJWB_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316620.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFSLMLVALFLSMAWMALTSA
                     GLVG"
     gene            555..740
                     /locus_tag="ACHJWB_005203"
     CDS             555..740
                     /locus_tag="ACHJWB_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4316621.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          205 a          178 c          220 g          192 t
ORIGIN      
        1 caggcttgtg ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg
       61 aatgagtgga aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa
      121 gcgaatatgc tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg
      181 gattgatggg gtggctaatg atatcagata aactcataac gctggtgaag agcctctgtg
      241 tacttgtcgg catttcattt tcactaatgc tggttgctct ttttctttcc atggcctgga
      301 tggcgttgac ttcggcaggg ctggtggggt gagcataaac cgaatgcttt ccgcgtttac
      361 cgttattctg ctggtggtct gtggtgcgct tagtctgggg ctgaatcatt accgcgataa
      421 cgccataacc tacaaagagc agcgcgataa aaaagtcagt gagctggagc tggcaaatgc
      481 aaccattact gatatgcagc agcgccagcg tgatgttgct gcacttgatg ccagatactc
      541 gagggaatta gccgatgcga gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg
      601 tcgtaagcgc ctgcgcatca acgccaactg tccaggctcc ttgcgtaaag cccccatcac
      661 ctccggcgtg gataatgcaa ccggtccccg actggcagaa gccgctgaac gggattattt
      721 catcctcaga gaacggctga tggcaatgca gaagcaactg gaaggagcac aggaatatat
      781 ccgtacccag tgtat
//
LOCUS       DAZFAH010000499          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFAH010000499 DAZFAH010000000
VERSION     DAZFAH010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.502"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>795
                     /gene="yjiC"
                     /locus_tag="ACHJWB_005204"
     CDS             <1..>795
                     /gene="yjiC"
                     /locus_tag="ACHJWB_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4316622.1"
                     /translation="NALQSQIITDDHFLHHPIVDSELIRKYERARLDTENIYLLPLVR
                     GNNHNYDGKSVVEIRKLDISKEYWPFNYVTETCRESDGITTTGRMLYRNLKITSALDD
                     IYGGICKKAHAATELAEGLRLNLFMKSPSYPVENYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDENAPLKHMTLQPSSLPELFELKNGE"
BASE COUNT          257 a          160 c          162 g          216 t
ORIGIN      
        1 gcaatgccct tcagagtcaa attattacag atgatcactt ccttcatcac ccaatagtcg
       61 acagtgagtt aatacgtaaa tatgaacgtg cacgcctgga tacggaaaat atctatcttc
      121 tgcctcttgt tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 agcttgatat tagtaaagag tactggccat ttaattatgt gacagaaaca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      301 tagacgatat atatggtggt atatgtaaaa aggcgcatgc tgccacagag ttagcagaag
      361 gattacggct aaatttattt atgaagagcc cttcctatcc tgttgagaac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttccaggtta cgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaaggattg
      541 atatctatat tgatcagata atgactgtct caggggtggc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatcccgg taccgcggag atccatgcaa aaatgggata taaagttatc ccgggtgatg
      721 aaaatgcacc actcaaacat atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaat
//
LOCUS       DAZFAH010000500          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFAH010000500 DAZFAH010000000
VERSION     DAZFAH010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.503"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..>794
                     /locus_tag="ACHJWB_005205"
     CDS             35..>794
                     /locus_tag="ACHJWB_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316623.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMV"
BASE COUNT          258 a          166 c          177 g          193 t
ORIGIN      
        1 ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat
       61 atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa
      121 gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc aactgacaga
      181 caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagctgc
      241 gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg
      301 caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac
      361 attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa
      421 tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt
      481 ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatacattgc agacaggaaa
      541 atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcaccga
      601 cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg
      661 ggctacctgg tcgccggtga attatgaata taaagtgctt ccaccattgc ccgacaagaa
      721 tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc aaaagaccta
      781 taaagcgatg gtaa
//
LOCUS       DAZFAH010000501          792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFAH010000501 DAZFAH010000000
VERSION     DAZFAH010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.504"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..287)
                     /locus_tag="ACHJWB_005206"
     CDS             complement(<1..287)
                     /locus_tag="ACHJWB_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4316624.1"
                     /translation="MLHKALRLIRQYHKESIVELSSSLGIPKDKIVSLESGVWSPSIE
                     VLERYASHFDIPVSSLVFFSESLGTQGRLSKRLRLNLAGKVLDVLEWVSNK"
     gene            complement(324..479)
                     /locus_tag="ACHJWB_005207"
     CDS             complement(324..479)
                     /locus_tag="ACHJWB_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316625.1"
                     /translation="MKNDKLTNEQQAQNKSNLKKWAGYAWKLIKWGIRLFDLVTRALD
                     YFEGGDE"
BASE COUNT          218 a          194 c          152 g          228 t
ORIGIN      
        1 tttttattac ttacccactc cagaacatcc agaactttac cggctaaatt taaacgcaat
       61 cgcttagaca aacggccttg cgtgcctaat gattccgaaa agaatactaa agaggatact
      121 ggtatgtcga aatgggaggc gtaacgctct aagacttcaa tcgacggtga ccaaactccg
      181 ctttcaagcg atactatttt gtcttttgga atacctaaag atgaagataa ctcaactatt
      241 gattctttat ggtattgcct tattaaacga agggctttat gtaacatagg caactccatg
      301 ctattgaaac tagggaggac gcattactca tcaccccctt cgaagtagtc cagtgctcga
      361 gtaactaaat caaacagtct aataccccat ttgattaatt tccaagcgta accagcccac
      421 tttttcaggt ttgacttgtt ctgcgcctgc tgttcgttgg ttaatttatc attcttcatg
      481 atttgctcct tatgcccata gtgtgcaaac ggtatgtctg ctccaacact atggttcgcc
      541 ctcataagga tgagattgaa cagcactagc cccctgctcc acgaaccctt ggtcgcccaa
      601 agggttcgaa tacccatacg ccatacctcc tgatcctgtg actcctgtcg ctggcagggg
      661 cgttgcgcgg ggtaagtgtt agaaagcgtt aactttccac gggtaaaaat acccaattga
      721 tggcgatata tctcactaac taaccaatgt ttgcatcata acaagtaaac cgttgtattt
      781 gctccccctg ta
//
LOCUS       DAZFAH010000502          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFAH010000502 DAZFAH010000000
VERSION     DAZFAH010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.505"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..480)
                     /gene="yhaB"
                     /locus_tag="ACHJWB_005208"
                     /pseudo
     CDS             complement(1..480)
                     /gene="yhaB"
                     /locus_tag="ACHJWB_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
BASE COUNT          229 a          149 c          160 g          253 t
ORIGIN      
        1 aagtaatgca ggttttatta aagtgcgtag ggggggttaa gatggcttct ttttgggtga
       61 actttgcgca atacttagaa atcgtctcct taatacattc cagctctttt tattgacggc
      121 tgagcaagta cattgttcac caaaaggtgc aaaagtgcct acatctaatg tttgtatgat
      181 ataatcatca tgttctcctg tagaaatatc tatgatgaat ggactcatat tttccggttg
      241 gattcggatc atgacagaag gaacatcgcc ttgatagaaa ttcaatgcag taattgcaga
      301 taatactctc cgcagttcat tacttatact gtaaatttta ttaacgtcat tggtggagac
      361 aatttctgca aactttcttt ttaccgtcca ataatcaata tttctattgt gattgttaac
      421 cactgcgtgc attggcggga ttgaagggtg aaaaatatgt gcaattggga agcctttcat
      481 tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc ttattttcct cgcagggttc
      541 tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca gtaaacgaag caacattctg
      601 cggcctacga cgatcaaaag ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat
      661 cgcggcgatc agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgtcgccta
      721 tgaggggaga agaatttatg gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga
      781 gaaaaagttg a
//
LOCUS       DAZFAH010000503          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFAH010000503 DAZFAH010000000
VERSION     DAZFAH010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.506"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..128
                     /gene="tnpB"
                     /locus_tag="ACHJWB_005209"
     CDS             <1..128
                     /gene="tnpB"
                     /locus_tag="ACHJWB_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4316626.1"
                     /translation="RFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            178..>791
                     /locus_tag="ACHJWB_005210"
     CDS             178..>791
                     /locus_tag="ACHJWB_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO4316627.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYG"
BASE COUNT          174 a          237 c          231 g          149 t
ORIGIN      
        1 ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg
       61 cgatgctgct ggaaggtatc gactggcgac agcctaagcg gctgctgacc tccctgacca
      121 tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata cgggcttatg
      181 aacgacatct cttctgacga catcttcctg ctgaaacagc gcctggccga acaggaagcg
      241 ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga ccatctgcag
      301 gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc
      361 cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga tacgctgact
      421 ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg
      481 ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcggcgcc gtgctgcccg
      541 aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct ggagttgatg
      601 cgtagcgcct tccgggttat ccggacggta cgggaaaaac atgcctgtac tcagtgcgat
      661 gccatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc cggaccgggg
      721 ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta tcgccagtca
      781 gaaatatacg g
//
LOCUS       DAZFAH010000504          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFAH010000504 DAZFAH010000000
VERSION     DAZFAH010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.507"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          277 a          188 c          122 g          203 t
ORIGIN      
        1 gacgcagata aaatgcacca ttttttcttc aaatacttct gtataatcgc cagccaatga
       61 tgagaatgga ttaaaataac acttcttcga caaacacaaa aacagccttc attttacatc
      121 atcatccagt ttcagttaac cccggacgag cgacagcgtt tcagtacagg aattaaaagc
      181 cagcttttgc tcagtaaaaa catactcaac taataatatc tcagcagata ttatcctgac
      241 caggacacct cacattactg aattctcttc cgacagtatc actgcacaaa taaaaaagaa
      301 cgggataaat attcagaccg gaagaatggt gtcacaaaag catttaatca ttacgcatga
      361 ccaggaaggg aaacacttaa ccacatggta tcaacagata tcattacgtg tgcactctta
      421 taaaaaacac acagaagaag taagataatt ataaacgtaa atttaatatc tcccactgac
      481 atctccccca caatacactg aaaaatactc acagcagtaa acacagacac acctgataat
      541 catgcagccg ataaatattt tcccctttac ggagaaatca gcctgctgtt taacaataac
      601 ggcgtatttt catggaagga gatcacccag taacggataa gtcctgatct ccttcatgtt
      661 aatacgcaac aatcccatcg attcaaaaca atttcagaca gatattatct ccgtgaatcc
      721 cccgccacct ttccggtgcg cggggttttg tcttttttca ccgggaatac atgtatgaat
      781 ccgtctgatg
//
LOCUS       DAZFAH010000505          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFAH010000505 DAZFAH010000000
VERSION     DAZFAH010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.508"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..129
                     /locus_tag="ACHJWB_005211"
     CDS             <1..129
                     /locus_tag="ACHJWB_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316628.1"
                     /translation="LLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            206..421
                     /locus_tag="ACHJWB_005212"
     CDS             206..421
                     /locus_tag="ACHJWB_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4316629.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            426..770
                     /locus_tag="ACHJWB_005213"
     CDS             426..770
                     /locus_tag="ACHJWB_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4316630.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
BASE COUNT          205 a          164 c          236 g          179 t
ORIGIN      
        1 ctgcttggtg tacaggcaga gccaccgact gaagcgggag ataccaccgc agagagcggt
       61 gaagttcagc ctgaatcacc ggtcgccgat gcgactgaac ctcaacccga atcggtcatg
      121 atgctgtaac ggggagtcag ggccatcagt aaacagctgc tggccttttt catgttgtga
      181 gcttccggat aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc
      241 atacaccacg tcagcggtag ggacgggata ctggttactg caactgctgg acaaagtctc
      301 tccgtcccag tgggtggcaa taggtgtact ggggagtctg ctgtttggcc tgctgacgta
      361 tctgactaac ctgtatttca aaatcagaga ggaccggcgt aaggcggcgc ggggagagta
      421 aagcgatgaa gaaaaaatac gaactggttg ttaaagggat aaataattac ccggataaga
      481 ttactgttac tgtggcactg gaaattggtg ggtatccgtc actgttgttg ccagatgtgg
      541 cgattagtct tgaccgtact gaaggtgcca cgctggagtt ttacgaagct gaggcgaaaa
      601 agcaggcgaa gcagtttttc atggatgttg ctgccgggtt atgtgaaggg gatggtccgt
      661 tgccggaaaa gcggcccatc attttagagg cgcaggatgt gttgataacc tacagaggaa
      721 aactaccggg aataattact ggttctctga agactccacc gctggcctga agacttaaca
      781 tatc
//
LOCUS       DAZFAH010000506          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFAH010000506 DAZFAH010000000
VERSION     DAZFAH010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.509"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>781
                     /locus_tag="ACHJWB_005214"
     CDS             <1..>781
                     /locus_tag="ACHJWB_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO4316631.1"
                     /translation="EHQTLSELFYKELEDKFSGKELATPLLKSFSENCRQNGRHIFSN
                     KDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEEYLMEILPQRSDTLDKQEL
                     NLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSS
                     AEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFELVMKAYFDKMESNGISVMAAIL
                     LVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSE"
BASE COUNT          280 a          149 c          141 g          211 t
ORIGIN      
        1 gaacaccaaa ccctgagtga attgttttat aaagaactcg aggataaatt ttctggcaag
       61 gagctggcga cgcctctatt aaaaagcttc tcagagaact gtagacaaaa tggtcgtcat
      121 atctttagca acaaggattt tgtcattaaa ttttccacgt ccgtcttaca agctgataag
      181 aaagaaatta cgataattaa taaaaacgaa aacacgacac tcactcaaac cattgctcca
      241 atatttgaag aatacctaat ggaaatttta cctcaacgct cagatactct tgataaacaa
      301 gaattaaacc taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt
      361 caatgttatt ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa
      421 tatattaatg atatttatca gaatgttgat tacaaacccc atcaagatga ttactcttca
      481 gctgaaaaat ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt
      541 tccagtcgtc ctgaggggcg ttgtgttgct gcctgtggtg atttcgagct agttatgaag
      601 gcatatttcg acaagatgga atcaaatggc atcagtgtta tggcagccat attattggtg
      661 gataaccatg ctttgacggc ccgactaaga ataaagaaca caactgaagg atgtacccat
      721 tacgtggttt cggtttatga tccaaatgta actaacgata aaataagaat catgagcgaa
      781 a
//
LOCUS       DAZFAH010000507          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFAH010000507 DAZFAH010000000
VERSION     DAZFAH010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.510"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>780
                     /locus_tag="ACHJWB_005215"
     CDS             <1..>780
                     /locus_tag="ACHJWB_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4316632.1"
                     /translation="ITKEEYFARKNGTYSGSQQNTGASDTTAQPGPVKVTADEVNKIM
                     QAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHESERNYQKAE
                     QNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEE
                     YQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADLSIKVNGHQEITSTSRT
                     CNHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT"
BASE COUNT          262 a          187 c          196 g          135 t
ORIGIN      
        1 aatcacgaaa gaagaatatt tcgcccgcaa aaacggtaca tactcaggtt cacaacaaaa
       61 tactggtgca tctgacacga ccgcacaacc agggccggta aaagttaccg ctgacgaagt
      121 aaacaaaatt atgcaggcag ccaatatcag ccagcctgac gccgataagt tgcttgctgc
      181 ctctcgcgga gaatttgttg cagggattag cgacccgaat gatccgaaat gggttaaggg
      241 gatccagacc cgcgattctg taaaccagaa ccagcatgaa tcggaacgga actaccaaaa
      301 agcggaacaa aacagcccaa atgcgttaca aaacgagcca gaaacgaaac agcctgaacc
      361 agtggcgcaa caggaagtgg aaaaagtctg caccgcctgc ggtcagaccg gcggcggcaa
      421 ctgccctgat tgtggcgcgg tgatgggcga cgcaacatac caggaaacat tcgatgaaga
      481 gtatcaggtt gaagttcagg aagatgatcc ggaggaaatg gaaggcgctg aacatccaca
      541 caaggagaac actggcggca atcagcatca cgatagcgat aatgaaactg gcgagacggc
      601 agatctctca attaaggtga acggtcatca agaaatcaca tccaccagca ggacgtgtaa
      661 ccatctaatg atcgaccttg aaaccatggg aaaaaatcct gatgccccga tcatctcaat
      721 aggtgcaata tttttcgatc cgcaaaccgg agatatggga ccggaattta gtaagactat
//
LOCUS       DAZFAH010000508          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFAH010000508 DAZFAH010000000
VERSION     DAZFAH010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.511"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(190..732)
                     /gene="fimA"
                     /locus_tag="ACHJWB_005216"
     CDS             complement(190..732)
                     /gene="fimA"
                     /locus_tag="ACHJWB_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4316633.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          240 a          164 c          155 g          220 t
ORIGIN      
        1 aaaaataaga aatataaaga aatgattgct ttggtttctt acaacacatc attccctttt
       61 catttaataa agcggcatga tacaaagtga acatgatttc gatttacatt gccaccatac
      121 cgcttcagac aaccggtaca ttactttgtt ataagacgtg atatataatt caaaacaacg
      181 tggttttgat tattcgtatc tcataataaa agtcgcgtca gcatttgctt gccctgctga
      241 tgcacttgta ccggtgcctt tataacgtgc agaaaaatga agaacgttgg tcccggggat
      301 caagttctgg ttggttgaga agctattccc atcaggtttg agaattgcgg atgtattatc
      361 aagtatttca ataccgacac ccgttgctgt tgttgtattg gtactggatg caatggccaa
      421 taaattatca ttgattgcat cagcctgacc agaaaatgca acggcagcat tagcggcaac
      481 aacaggatcg cagtcgttca actgaatggt gaatggaatt aatgcagagg tattaccaac
      541 agcattgaaa atatcggtac gatattgacc gagtgtgaca acctggtctg ccgagtcagt
      601 attcactgaa caggcagcat tcaccagttc gccttcaaag tgaattgtac cgccatctac
      661 aacggcagca taactacccg tagcggcgaa tagtgcggca gccagcgcag acgaaataaa
      721 tcttaatttc atatatattc cttcaatctc atttatcgac tccacatccg tatataacc
//
LOCUS       DAZFAH010000509          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFAH010000509 DAZFAH010000000
VERSION     DAZFAH010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.512"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            211..775
                     /locus_tag="ACHJWB_005217"
                     /pseudo
     CDS             211..775
                     /locus_tag="ACHJWB_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014622.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          244 a          145 c          202 g          184 t
ORIGIN      
        1 acaatgagca ataagaaaat tgaatgcctg gagaagagtg aagtagaatg ggctagtagg
       61 gtttgcgaca gtatcacaga caaggaaagg gaaaatctga aggcattaat atgtagcaaa
      121 ccagctaaat cagttaatac aggcaaaaga ccagcgttag gatattgaaa ccggcactgc
      181 actatatcac tattacaata ttctgagtga ctagagcaac tgctccgcca tccgggctag
      241 cagtatgacg acgaatccga actgcactac aactggcagc ggtactacaa tccgggacag
      301 ggacgatata tcacccagga cccgataggg ctggcggggg gattaaaccc gtacagttat
      361 gttatgaaca atcctgtgca atggattgat ccattgggat tagctcatac ttcaggaaaa
      421 tggaaggact gtggtaaagg ttgtcgaatc cgaatagatg gcgatgctgt tggtgacgga
      481 agacatttgc attgggagtg taaaaatggg agtggtgtaa tgggggaatt tggaggtatg
      541 tcccatggtg aggattactc gtcagcacca aatagcatta agaaatgtgc aaaaaaatat
      601 ggtttcgagc cagaaccaag taaagatctg tcatgcaaaa cctccgggct atcaactact
      661 gatgcggctc ttgcaactgg tgctacaatt ggcgcagggt atgttatata tagagtgatt
      721 cgatttatac cttcacttgc accacctctt tggtggtcaa tacctgcaaa cgttg
//
LOCUS       DAZFAH010000510          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFAH010000510 DAZFAH010000000
VERSION     DAZFAH010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.513"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..147
                     /locus_tag="ACHJWB_005218"
     CDS             <1..147
                     /locus_tag="ACHJWB_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316634.1"
                     /translation="VKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTES
                     QHEE"
     gene            92..>767
                     /locus_tag="ACHJWB_005219"
     CDS             92..>767
                     /locus_tag="ACHJWB_005219"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFO4316635.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNS"
BASE COUNT          205 a          173 c          218 g          171 t
ORIGIN      
        1 gtcaaacgtc gccgcaaggc tgaaattgat gaaaacagca gactggggct ggtctttgat
       61 actgaccccg tcaacgacaa aggaggcaac agtgccggaa ctgaacgaca gtatcagcgc
      121 gacaccgaaa gccagcatga agaataaatc ctggttcagg atgcaggctg gggggccggg
      181 tgacgcggat atttatattt atgacgagat tggtttctgg ggagttaccg cgaagcagtt
      241 tgtcagcgaa ctgaatgcac tgggtgatat cacccacatt aatctccata tcaattcacc
      301 gggtggcgat gtctttgaag gcatcgccat ttttaatgcc ctgaaaaatc agggggcgac
      361 cattaccgtg tatgtggatg gcgttgccgc ctcgatggca tctgtgattg cgatggccgg
      421 tgatacggtc attatgccgg aaaatgcctt catgatgatc cataagccat ggggattcag
      481 tggcggggat gctgaggata tgcgcagtta tgccgatttg ctggataaag tcgaatcggt
      541 actgttgcca gcctatgcgc agaaaaccgg aaaaaccacc gatgaaattg ccgccatgct
      601 ggcggatgaa acctggatgt ccggtgccga atgtctggca cacggatttg ctgaccaggt
      661 gacacccgct gttgaggcaa tggcatgtat tcagtcaaaa cgtacagagg aatttaaaaa
      721 gatgccggaa tccatccgaa acatgattac tccgccacgc aacagtg
//
LOCUS       DAZFAH010000511          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFAH010000511 DAZFAH010000000
VERSION     DAZFAH010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.514"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>755
                     /locus_tag="ACHJWB_005220"
     CDS             <1..>755
                     /locus_tag="ACHJWB_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4316636.1"
                     /translation="NYMTNLNAVFQFGVDNSYLADNPFKGISPLKESRTIPDPLSREE
                     FIRLIDACRNQQAKNLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDHFTV
                     PKTQAGTNRVIHLIKPAIDALQSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPE
                     VSARVKNYGDHFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIAN
                     QMGHADAQMVFQVYGKWMSENNNAQVTLLNTQL"
BASE COUNT          247 a          151 c          171 g          186 t
ORIGIN      
        1 ataattacat gacaaatctt aacgccgtgt tccagtttgg tgttgataac agctacctgg
       61 cagataatcc gtttaaaggg atatcgccat taaaggaatc aagaaccatt ccagatcctc
      121 tttcgcgtga ggaatttatc cgccttatag atgcgtgcag aaatcagcaa gccaaaaatt
      181 tgtggtgtgt ttctgtttat actggcgttc gccctggtga gttgtgtgca cttggatggg
      241 aggacataga tctgaaaaat ggaacaatga tgatcaggag aaatttagca aaagaccatt
      301 ttacggtacc aaaaacacag gcgggaacca atcgtgtaat tcaccttatt aagccagcaa
      361 tcgacgcact ccagagtcag atgacactaa cgagactgag caaagagcat atcattgatg
      421 ttcacctcag agagtatggc agaacagaaa aacaaaaatg cacctttgtt tttcaacctg
      481 aagtgtcagc gagagtaaaa aattatggtg accattttac tgttgactca ataagacaga
      541 tgtgggacgc agcaataaaa cgagctggac tccgccaccg aaaatcatat cagtcgagac
      601 atacttatgc ctgctggtcg ctaacagctg gtgctaatcc tgcattcata gcaaaccaga
      661 tggggcatgc cgatgcgcaa atggtgtttc aggtgtacgg aaaatggatg tcagaaaata
      721 ataatgcaca ggtaacgctg ttaaatacac agcta
//
LOCUS       DAZFAH010000512          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFAH010000512 DAZFAH010000000
VERSION     DAZFAH010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.515"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..380)
                     /locus_tag="ACHJWB_005221"
     CDS             complement(<1..380)
                     /locus_tag="ACHJWB_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4316637.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLDEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIR"
     gene            complement(380..>754)
                     /locus_tag="ACHJWB_005222"
     CDS             complement(380..>754)
                     /locus_tag="ACHJWB_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316638.1"
                     /translation="HSLSSSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGV
                     QGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITV
                     NAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          188 a          188 c          165 g          213 t
ORIGIN      
        1 cggatccgga acagtttttc tgcttctgca tctttcaccc aggctgtgcc gttccacttc
       61 tgaaactccc cttccggcga taaccaggta acattttccg gtaacggacc gagttcagaa
      121 ataaataacg cgtcccctga cgctacgtca taaaccgttt taccccgatg gtcttcatcg
      181 agatgccacg atgccttatc actgttgaaa acagccacaa agccagcagg aatatctggt
      241 ggtgcaatat cggtactgtt tgctggcaga cctgtatgag gcggaatata tgcgtcacct
      301 tcaccaataa attcattagt tccggccagc agattataaa tttttatggt ccgtggttgt
      361 tcactcattc tgaatgccat tatgcaagcc tcacaatata gttaaatgcg atgtttttga
      421 cggtgttttc cgcgttacca gcagcgttaa cggtgatggt gtgtccatgt gaaccaatcg
      481 caacggagtg cgtatgagca ccaataccga cagtatgcgc gtgtgcacct gcagatgctg
      541 ctgtgccgga cagtgaatgg ctatgattac catctgtact ggtattcgct aaccaccccg
      601 tagacatacc tactgagcct tgtacacccc aggtattttg acctgagctt gtataaccat
      661 attgataagt atctttaaaa acactggggt taaatcgacg gccatctcta tggctgtgat
      721 taccagctgc attcgtgctg ctacttaaac tatg
//
LOCUS       DAZFAH010000513          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFAH010000513 DAZFAH010000000
VERSION     DAZFAH010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.516"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..371)
                     /locus_tag="ACHJWB_005223"
     CDS             complement(<1..371)
                     /locus_tag="ACHJWB_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4316639.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVSEDDVALMMASAGIS"
     gene            complement(398..>754)
                     /locus_tag="ACHJWB_005224"
     CDS             complement(398..>754)
                     /locus_tag="ACHJWB_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4316640.1"
                     /translation="IEHLALLKRRAEQAESSGNLQVSVEDLVRTGAFLVAMSLWHNHP
                     QKTASPSMNEAVMQIEQEVLTTWPADAIARAEDVVLRLSGMSGAVHADTDSTEVAKNN
                     ALTDDDFSAGKSSTAS"
BASE COUNT          172 a          238 c          182 g          162 t
ORIGIN      
        1 gaaatccccg ctgaagccat cattaatgcg acatcatcct cgctgacatc cgcagacgat
       61 aaaatctcac gccctccgtc cccaccaaac cgtacgcctc cggcaagtcc tgccgctttc
      121 tgcatcagca ttttttcctc atccggcatc tccgtctgcg cttcctcacg ccggggggca
      181 agcagactga aatcagaggg atgcatatcc ggatcgcaaa aaaacaggct gagtacggcg
      241 tacgtcagcc cggaaaaatg catatccagt tgggtatcga gaaaataatg cgtacggtaa
      301 aaatgtcgcc agtcggcata ttcggtggat gtcatcccgg caagcatggc gcgccagtca
      361 ggcctcccca tctcacgcgc cagtctgagg gcaaaattca gctcgccgtc gaagactttc
      421 ccgcagaaaa atcatcatca gtcagcgcgt tatttttcgc cacttcggtg ctgtcagtat
      481 ccgcatgaac agccccgctc atcccggaca gacgcaacac cacatcttcc gcccgggcaa
      541 tggcatccgc aggccaggtg gtgagcacct cctgttcgat ctgcatcaca gcctcattca
      601 ttgacggtga tgccgttttc tgcggatggt tatgccacag ggacatcgcc accagaaacg
      661 cgccggttct gacgagatct tccacgctta cctgcaggtt gccgctggat tctgcctgtt
      721 ctgcacgccg tttcaggagg gcaagatgct caat
//
LOCUS       DAZFAH010000514          751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFAH010000514 DAZFAH010000000
VERSION     DAZFAH010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.517"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..669)
                     /locus_tag="ACHJWB_005225"
     CDS             complement(58..669)
                     /locus_tag="ACHJWB_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO4316641.1"
                     /translation="MFSPTAMVGRALAQAVTQTFRPAVTKVATQAGMGASGMRFTPMQ
                     SNLMINHGKLTTQLLQAVAKQTGSSDTQQWFRQEQITFLSRAVNKTVDDYCMSNNSAI
                     SKETKCRIFKEVESAIQQPLDMNCAQSSISHFLQSNKYFNQKVDEQCGKGVDPITRFN
                     TQTRMIEQASREIFEQNFGTVKVSDIKALTEKAIAENVQDTRL"
BASE COUNT          212 a          170 c          133 g          236 t
ORIGIN      
        1 tttaagcgtc tatagagacc tttttgcatc aagaaaaaac aaagcagtga gtccccccta
       61 cagtcgagta tcctgcacat tttctgcaat cgccttctca gttagagcct tgatatcact
      121 gactttcacg gtgccaaaat tctgttcaaa gatttctcgc gaggcctgtt ctatcatcct
      181 ggtttgagtg ttaaaacgtg taatgggatc cactccttta ccacactgct catcaacttt
      241 ctgattaaag tatttattgg attgcaaaaa gtgactaatt gatgattgtg cacagttcat
      301 atcgagaggc tgctgtatcg cgctttcaac ctctttaaaa atgcgacatt tagtctcctt
      361 actaattgcc gaattattac tcatgcaata gtcatccaca gttttgttta ctgctctgga
      421 tagaaaagta atctgctctt gcctgaacca ttgctgggta tcgctactac cagtttgttt
      481 ggctacggcc tgtagtagct gggtagtcag tttgccatga ttaatcataa ggttcgactg
      541 cataggtgtg aacctcatac cactggctcc catgcccgct tgcgtcgcaa ccttggttac
      601 agcgggtcta aaagtttgtg taaccgcctg agctaacgct ctacctacca ttgccgttgg
      661 actaaacatg ctataaacct ttataaatta cataaaaaca tttacaccta taaaaataaa
      721 acaaacaaat aaaactataa cattatttta t
//
LOCUS       DAZFAH010000515          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFAH010000515 DAZFAH010000000
VERSION     DAZFAH010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.518"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>739
                     /locus_tag="ACHJWB_005226"
     CDS             <1..>739
                     /locus_tag="ACHJWB_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFO4316642.1"
                     /translation="HALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQI
                     HPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIA
                     QALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMP
                     GVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRA
                     LFLSAFAALRDPLSRAYYTRKMSQGKR"
BASE COUNT          150 a          249 c          196 g          144 t
ORIGIN      
        1 ctcacgcgct acgcacgctg aaactggctg acgagcaaat cgccgaactc tccatgctct
       61 gcggcttcga tgatgatctt gccgcacaga caacgcaggc cagcaaccgt atccgcggcc
      121 ttctgaccca gatacatccg gcactggagc gcgttctcgg tccgagactt gatcacccgg
      181 cggtactcga tcttctccag cgatatccct caccagaaaa actcgcttcg ctgggtgaga
      241 agaagctggc agcccagctc tgcaaacttg cgcctcgtct gggtaaacgc cttgcagcag
      301 acatagctca ggcactggcc gaacaaaccg tcgtcgttcc cggcacgaat gccgctgccg
      361 tagtactgcc acgtctggca ctccagctca tcacgctgcg taagcaaaga gacgaggtgg
      421 cgcttgaggt agaacagcga gttcttgctc accctcttta cccggtcctg accagtatgc
      481 ccggagtcgg tgtcaggacc gcagccagac tcctcaccga ggtcgcctgc cgcgccttcg
      541 cctctgccgc acatctcgct gcttatgctg gccttgcgcc ggtaactcgg cgatccggct
      601 cgtcaatacg cggtgagcat ccctcgcgac ggggtaataa agctctcaaa cgggcgttgt
      661 tcctgtcggc cttcgccgcg ctcagggatc cgctctccag ggcttactac acccgcaaaa
      721 tgagtcaggg aaaacgaca
//
LOCUS       DAZFAH010000516          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFAH010000516 DAZFAH010000000
VERSION     DAZFAH010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.519"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..504
                     /locus_tag="ACHJWB_005227"
     CDS             <1..504
                     /locus_tag="ACHJWB_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316643.1"
                     /translation="MLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAILAAAAN
                     FHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAG
                     SPAQMRRAEGISFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDEIQAQMRDGG
                     VFSGGRR"
     gene            501..>736
                     /locus_tag="ACHJWB_005228"
     CDS             501..>736
                     /locus_tag="ACHJWB_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316644.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYA"
BASE COUNT          144 a          165 c          274 g          153 t
ORIGIN      
        1 atgctgacag agatttttct gaagcaggcc atggtgggga ttgtcgggag tattggcagc
       61 gccatgggtg gtgctttcgg tggtggggcg tctgcctcca cggggacggc cattctggct
      121 gcggcggcga acttccattt cgcgaccggg ggatttacgg gaacgggggg caaatacgaa
      181 cctgccggta ttgtccaccg cggggagttt gtcttcacga aggaggcaac cagccggatt
      241 ggcgtcggca acctgtatcg tctgatgcgc gggtatgcgg aaggtggtta tgtgggcggt
      301 gccggaagtc cggcgcagat gcggcgggcg gaaggcatta gttttaatca gaacaatcac
      361 gtggtgattc agaacgacgg tatcaacgga caggcagggc cgcagctgat gaaagcggtg
      421 tatgacatgg cccgcaaggg ggcgcaggat gagattcagg cgcagatgcg tgatggcggc
      481 gtattttccg gaggcaggcg atgaaaacct ttcgctggaa agtgaagccg gatatggagg
      541 tgaactcgca gccgtcggtg cgtgaagtgc gttttggtga cgggtattcg cagcgtatgg
      601 ctgcggggct gaatgctgac ctgaaaacat accgtgtgac gctttccgtg acccgggagg
      661 aggcccgaca tctggaggcg ttcctggcag agcacggtgg ctggaaggcg tttctgtgga
      721 caccgcctta tgcctg
//
LOCUS       DAZFAH010000517          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFAH010000517 DAZFAH010000000
VERSION     DAZFAH010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.520"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..467)
                     /locus_tag="ACHJWB_005229"
     CDS             complement(153..467)
                     /locus_tag="ACHJWB_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4316645.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          221 a          187 c          106 g          211 t
ORIGIN      
        1 aaaaatattc tcaatttgta cccaacaaag acaaacacga ccagagaacc tgttcagaca
       61 ggtttactta aacgacttat atatgacaca aaaagcgacc actaaagtcg ctttttctta
      121 tggtaacagg caataacgct ctcagatatt ttttagcatt tttttgaccg cgcgtttccg
      181 gacgtattct gttctcctgt ccctttatat cgtcggaata cccgccgctc ttcaaatccc
      241 attcccaact cagaatgtag tctgttgacc gcttgtttta tttcggtcag gttcaccggt
      301 gaaaccggag tccggcgcgc cttacgcaaa cactctgctc gtttctgtgc cgccactttt
      361 cttttctggt catcacttag ctgtaccatc acttttgccc atcgttcagc tgctctccgg
      421 tacagtcctt ttttctccag acattctgcc acgtgatcat gtagcataag tgacctccga
      481 ttatctacag actgccatcc tgaatttacc ttcccttaat gaaataacaa taaaaaacaa
      541 accacgcaaa aacaataaaa caacacacaa aaaaaactaa ataacaaaca aaataatcac
      601 cttattttat tattttttga gggagcaatt actgaacaaa aaacgctgac tatatactca
      661 aaaccaaaca actattctgc caatcaggta tcatggcaac acacggaatt accgtgtttt
      721 tgcct
//
LOCUS       DAZFAH010000518          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFAH010000518 DAZFAH010000000
VERSION     DAZFAH010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.521"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..408
                     /locus_tag="ACHJWB_005230"
     CDS             <1..408
                     /locus_tag="ACHJWB_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4316646.1"
                     /translation="YVMALYWPVSSLPQGVTLTGDAGNNLLASFYIQTDAKDLNVMYH
                     NAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTF
                     PVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            555..>725
                     /locus_tag="ACHJWB_005231"
     CDS             555..>725
                     /locus_tag="ACHJWB_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4316647.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDP"
BASE COUNT          212 a          159 c          175 g          179 t
ORIGIN      
        1 tatgtcatgg cgctttactg gccggtttcc tctctgccgc agggtgtcac cctgacaggg
       61 gatgcaggga ataatctgct ggcatcgttc tacatccaga cagacgcaaa agacctgaat
      121 gtgatgtacc acaatgcaaa agtggcaaca aataaccaga aactgggaac ctttggtgca
      181 tttgataatg aatggcatac gctggccttc cgttttgcag gaaataacag ccttcaggtt
      241 attccggtta ttgatggtca ggatggtgcc gcgtttaccc tgacgcagtc accagtagga
      301 actttcccgg tggacaaact ccgcgtaacg gatatcacaa aaaatgcaac ttacccggtg
      361 ctgattgaca gcattgtggt ggaagtgaaa aaagcggtaa cagaataaaa aaatcccgcc
      421 ggtcatgata tggctggcgg gagctaccta acccataaag gaaaataaaa atactcccga
      481 aggagtggtt atcagtcgct tcgatgtttt tacccgataa cgaattatgt agtcaatatc
      541 aaaggtaagg ttatatgaca ttcgttcata caatgctgct ttatttctgc gctgtggtca
      601 gtgcgttata tctggtgagc ggcggttata aggtcatcag aaattatatt cgccgtaaaa
      661 ttgatgatgc ggcagctgaa aaactcagta aaacagcaca ggcaccgtca tccccaaacg
      721 acccg
//
LOCUS       DAZFAH010000519          718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFAH010000519 DAZFAH010000000
VERSION     DAZFAH010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.522"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..180
                     /locus_tag="ACHJWB_005232"
                     /pseudo
     CDS             91..180
                     /locus_tag="ACHJWB_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            655..>718
                     /gene="ykgR"
                     /locus_tag="ACHJWB_005233"
     CDS             655..>718
                     /gene="ykgR"
                     /locus_tag="ACHJWB_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO4316648.1"
                     /translation="MKENKVQQISHKLINIVVFVA"
BASE COUNT          231 a          157 c          128 g          202 t
ORIGIN      
        1 agatttatga atttcgttca taacccctga caaatcctgc cgtctaatcg agcttttccc
       61 tttacgttat gcttctcatg aacgataaca caacttgttc atgaattaac cattccggat
      121 aaactatggg atcaaattca ggcatcgaaa ggaactgact ggtttccgtt aatgaattaa
      181 atatctatta ctctcccgag agtaaaacta tcaaaccaat gagtaaccga tacagcctcg
      241 caaattcttt cagataatta acccgtcact gcggcacgtt ctctcgtaag gcgtgcctgc
      301 agtgtaatcc agtatgtatc tggcatagtt gacggtttaa tagaactcaa ttcctcgccc
      361 aggttaccaa caacgatatc ttgtataaca ccccaggaga tacgacatta tgatgagatg
      421 cgatatactc ctcagcacca atcaacgccc ggactgggcc agtcttgaga tcagactcat
      481 tcccggaact gacttcaggc gacatatatc aatgacgttg gtgcaataaa agatagccga
      541 tgtaatacat ctattcaaat ttgtgataca tgtcaaaaaa attggttgac caaactcgtt
      601 atttatataa gggcacttac gaagtgcact cttttttaaa gcgaggaagt accaatgaaa
      661 gagaataaag tacaacaaat cagtcataaa ctgattaata tcgttgtttt tgtcgcaa
//
LOCUS       DAZFAH010000520          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFAH010000520 DAZFAH010000000
VERSION     DAZFAH010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.523"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..683
                     /gene="fimE"
                     /locus_tag="ACHJWB_005234"
     CDS             87..683
                     /gene="fimE"
                     /locus_tag="ACHJWB_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4316649.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          195 a          136 c          175 g          210 t
ORIGIN      
        1 ctttttatct attgggctaa ttttgaccga ttgaggtttc ctataggtat tcattcaaat
       61 atatctcagt taggagtact actattgtga gtaaacgtcg ttatcttacc ggtaaagaag
      121 ttcaggccat gatgcaggcg gtttgttacg gggcaacggg agccagagat tattgtctta
      181 ttctgttggc atatcggcat gggatgcgta ttagtgaact gcttgatctg cattatcagg
      241 accttgacct taatgaaggt agaataaata ttcgccgact gaagaacgga ttttctaccg
      301 ttcacccgtt acgttttgat gagcgtgaag ccgtggaacg atggacccag gaacgtgcta
      361 actggaaagg cgctgaccgg actgacgcta tatttatttc tcgccgcggg agtcggcttt
      421 ctcgccagca ggcctatcgc attattcgcg atgccggtat tgaagctgga accgtaacgc
      481 agactcatcc tcatatgtta aggcatgctt gcggttatga actggcggag cgtggtgcag
      541 atactcgttt aattcaggat tatctcgggc atcgaaatat tcgccatact gtgcgttata
      601 ccgccagtaa tgctgctcgt tttgccggat tatgggaaag aaataatctc ataaacgaaa
      661 aattaaaaag agaagaggtt tgatttaact tattgataat aaagttaaaa aacaaa
//
LOCUS       DAZFAH010000521          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFAH010000521 DAZFAH010000000
VERSION     DAZFAH010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.524"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..287
                     /locus_tag="ACHJWB_005235"
     CDS             <1..287
                     /locus_tag="ACHJWB_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316650.1"
                     /translation="IAVLNGFRTEKTSPLYFEKAVNGYTETQADSGAVMYALYFSCEE
                     MIAPLTDTSSLDDFLRHYETFVEPPGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            288..533
                     /locus_tag="ACHJWB_005236"
     CDS             288..533
                     /locus_tag="ACHJWB_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4316651.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            530..>715
                     /locus_tag="ACHJWB_005237"
     CDS             530..>715
                     /locus_tag="ACHJWB_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316652.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRIT"
BASE COUNT          170 a          187 c          207 g          151 t
ORIGIN      
        1 tgattgccgt gcttaacggc ttcagaacag aaaaaacctc accgctttac tttgaaaagg
       61 cggtcaacgg ttacaccgaa acccaggcgg acagtggtgc ggtgatgtat gcgctttatt
      121 tctcctgcga ggaaatgatc gcaccgctga ccgataccag ctcgctggac gacttcctgc
      181 gtcattacga aacctttgtg gagccgcccg gtacgccgcc gtttgaggca catattgatt
      241 tgccggggaa cacgacaacg gcggtcgaac cgccggagga actgtaaatg aaaaccatct
      301 ttatcaaacc cgcgccggga cgcctgattc gtgatcccga cacaatgcgc ccgctggcac
      361 aggaggggga ggaaaagccc tttacgccgt tctggtgtcg gcgtcttgat gacggcgatg
      421 tcattcaggc tgaaaaagcc gctgaagagg ctccggctgt atctgcggat gcaacgactg
      481 cgacagaaaa accggtcgcc caaccggcat ccgataagga gaaaccgcaa tgattaattt
      541 tgacgggatc ggtaatgacc gccgtatacc gctgattgag gttgagttta ataactccat
      601 ggctgtcacc ggcactccgg cacagcgtca gtgtgtgctg ctgtttggtc aggcgagaat
      661 gaaagagaat gccgtggacg gtgccggtgt tctggatgtt ccggtcagga tcacc
//
LOCUS       DAZFAH010000522          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFAH010000522 DAZFAH010000000
VERSION     DAZFAH010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.525"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..226
                     /locus_tag="ACHJWB_005238"
     tRNA            151..226
                     /locus_tag="ACHJWB_005238"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Ile,seq:cat)
BASE COUNT          203 a          138 c          167 g          204 t
ORIGIN      
        1 tatcgacttt gcgaatggaa gcggtgtcgg gttttatgtc gcgtgtaatg acgagcaagg
       61 atgaatgaga acgcataaaa atctgttatt tacatcatat gatcatcatg tttattgcgt
      121 ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt agagcaggcg
      181 actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc attatttacg
      241 tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt catgactaac
      301 gccatttcaa ggaagaagtg cggtgggttg tttcgaatga gggctggtgt tttggtacgt
      361 ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct ggaaaaaatg
      421 cccgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa cgataaagcc
      481 agagtattat catttattat accccttaat ttaatcaggt taattactca aatcactgaa
      541 ctattctctg ttcgctcact gatgatgagt gagtaccaca accaatgtat gtagaacaat
      601 gccgtagagt aaggatgaaa tgtctctgcc acgccttaaa catctactac tgaccagaac
      661 acccgccgca ggcagatgag ttttgtgagc gttacaggag cgtggctatg ct
//
LOCUS       DAZFAH010000523          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFAH010000523 DAZFAH010000000
VERSION     DAZFAH010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.526"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..141
                     /locus_tag="ACHJWB_005239"
     CDS             <1..141
                     /locus_tag="ACHJWB_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316653.1"
                     /translation="AEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDL
                     AA"
     gene            complement(184..523)
                     /locus_tag="ACHJWB_005240"
                     /pseudo
     CDS             complement(184..523)
                     /locus_tag="ACHJWB_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          182 a          174 c          171 g          179 t
ORIGIN      
        1 gcagaagtgg aactggccac actcacgtgg gtggactggt ataacaatcg acgattgctg
       61 gaaaggctgg gccatactcc tccggcagaa gcagaaaaag cttattatgc ttccatcgga
      121 aacgatgatc tggcagcctg agttcacaga taaaacactc tccaggaaac ccggggcggt
      181 tcaaacctga ttctggaaaa ggactttgtg gattgttgct attgccagtg agattgaatg
      241 aagtcgtttt aactcgcttt gaatacgtct tgcccccaga tttcgctgag aacgtaactc
      301 cagaatcaga gcgacttcgc aagtgccggt tttcgttgat ggtgaacgct tggggcgacg
      361 gctgtggctt tccagaccag cgatgccctg agccagatat ctgcgccacc attttacgca
      421 atgtgggccg agaaatgcca cagcgacggc atacaaatcc cgcatcaccg gacgtttcat
      481 agagttttac ccactgtagc cgctgctgaa tttccctgtt cataatttgg cattagttca
      541 gctcaatccc gacttaaaaa gtccctacag gatgttccgg acctgaggca cagttcatat
      601 ccttatccat aaaaaattta gctatgtcta tagcattaat cttgacaaga cgacgttagg
      661 gcctggcgat gaatcatttc ccctgccaga tatcctttgg ggtgca
//
LOCUS       DAZFAH010000524          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFAH010000524 DAZFAH010000000
VERSION     DAZFAH010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.527"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..638
                     /locus_tag="ACHJWB_005241"
     CDS             <1..638
                     /locus_tag="ACHJWB_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO4316654.1"
                     /translation="TAMASAAPALGAMAVVAGPMGYDLLANDGRAGKALSDGQLINSQ
                     AEMCYWTSSCPLDSPHENDNSSGSQPNVGKDLTDEEKNCLAGGSTGSPGGWGPEDENS
                     ARDRMQHERYKDELRQKMGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATG
                     EKVGGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
BASE COUNT          210 a          120 c          201 g          161 t
ORIGIN      
        1 cgacggctat ggcgtctgct gcccctgctc ttggagcaat ggctgtggtc gctgggccaa
       61 tgggatatga ccttctcgct aatgatgggc gtgccgggaa ggctctgagc gatgggcagt
      121 taataaattc tcaggctgaa atgtgttact ggaccagtag ttgtccttta gatagcccac
      181 atgagaatga taattcgtca gggagtcagc cgaatgttgg gaaggatttg acggatgaag
      241 agaagaactg cctggccggc ggcagtacgg gaagcccggg aggatgggga ccagaagatg
      301 agaatagcgc aagggatcgt atgcagcatg aacggtataa agatgaattg cgacagaaaa
      361 tgggaaaacc cgacgttaca gattcaaatt tgaaaaatat tatagacgat ctttatcggc
      421 caaatgcaaa agtaggaagt ggaagcacag cagatgctgt tcgttatgaa ttggccacag
      481 gtgaaaaagt tggtggtaga ggacatgtat tgaaagcaca ggattataca agggctttac
      541 aggattggtt ggataagaat ccaacagcat ctccagggga tcgagcagca gcagaaaacg
      601 ttcttcagga tttacagaat gctttgggag gcaagtaatg aaccaatata ttgctagtta
      661 tatagaaaaa atggcatcat tttccactgt tt
//
LOCUS       DAZFAH010000525          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFAH010000525 DAZFAH010000000
VERSION     DAZFAH010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.528"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>689)
                     /locus_tag="ACHJWB_005242"
     CDS             complement(<1..>689)
                     /locus_tag="ACHJWB_005242"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4316655.1"
                     /translation="AVFNEPLLLEPAYARVFFCALGREMGAASLSVPQQQVQLDAPGM
                     LAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGYDGIVACLQQ
                     AMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTACSAAMLLASA
                     CSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQFEALPAEVR
                     QDMQQRIDAARR"
BASE COUNT          145 a          233 c          190 g          121 t
ORIGIN      
        1 cggcgcgccg catcaatgcg ctgctgcatg tcctggcgaa cctctgccgg taaggcttca
       61 aactgattgc catccacctt gtgcgcccct gagtaaatca gcgtgatatc cacaccggcc
      121 tgcgccagat gaccggcata gctgacatgg ctcatcatca cgccaatgga gccgatacgg
      181 gatgtctggg taaccagccg tcgggagcag gccgacgcca gcagcatggc tgcagaacag
      241 gccgtgtcat tgcacagtgc ccagaccggc ttctgctgac ggaggcggta aatcatgtca
      301 gcgcagtcaa acgcgccggc ggcctgcccg cccggactgt caatgtccag cagtacgccc
      361 cgcacctggc tatccgccat tgcctgctga agacaggcga caatgccgtc atagcctgtc
      421 attccggaaa atggccgcat accccccagc cggtgcacca gcgtgccggt caccggcagt
      481 acagcaatac cgttcaccac ccggtaaaca cgggccggtc gtttacctcc ggccatgtac
      541 tcgtccgttt cagccagcat tccgggagca tcaagctgta cctgctgctg tggtaccgaa
      601 agacttgctg cccccatctc gcgcccgagc gcgcaaaaga aaacccgcgc ataggcgggc
      661 tccagaagca gcggttcatt gaatactgc
//
LOCUS       DAZFAH010000526          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFAH010000526 DAZFAH010000000
VERSION     DAZFAH010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.529"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>689
                     /gene="ycjM"
                     /locus_tag="ACHJWB_005243"
     CDS             <1..>689
                     /gene="ycjM"
                     /locus_tag="ACHJWB_005243"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO4316656.1"
                     /translation="AYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLP
                     SSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYE
                     INVTYMDALSCRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLG
                     YNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTIN
                     SSVMRIQRSNA"
BASE COUNT          193 a          155 c          167 g          174 t
ORIGIN      
        1 tgcttacttt ggcgcaggcg atgacgaagc acatatggtg taccagttct cgctgccgcc
       61 actggtgctg catgcggtgc aaaaacagaa cgttgaggcg ctttgtgcgt gggcgcaaaa
      121 cctgacacta ccttccagca acaccacctg gtttaacttc ctcgcctctc acgatggcat
      181 cgggctaaac ccgctacggg gcttgttgcc tgaaagcgaa atattagagc tggtcgaggc
      241 gttacagcag gaaggtgcat tagtaaactg gaaaaataat cccgacggta cacgcagtcc
      301 gtatgaaata aatgtgacct atatggatgc gttaagctgc cgtgagagta gcgatgaaga
      361 acgttgcgcc aggtttattc tcgcccatgc gattttgtta agtttccccg gtgtgccagc
      421 gatatatatt caaagtattc ttggctcgcg taatgattac gcaggtgtcg aaaaactcgg
      481 atataaccgt gcgattaacc gtaaaaaata tcacagtaaa gagataaccc gagaactgaa
      541 cgatgaagct acattaaggc atgcggtata tcatgagttg tcgcgtttaa ttacacttcg
      601 tcgcagccat aacgagtttc atccggataa taattttacc atcgatacga ttaattcatc
      661 cgtaatgcgt attcaaagaa gtaacgctg
//
LOCUS       DAZFAH010000527          685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFAH010000527 DAZFAH010000000
VERSION     DAZFAH010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.530"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHJWB_005244"
     CDS             complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHJWB_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFO4316657.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPIEFWIDGQPTPYEADQPLPIGKVIDIAGCGFIIGDIDHALP
                     LSDMPERFSARNQRKKRLILAGVIGAAIALSGAIGSYVLLSPKAELPTFTRADVYQQL
                     KENKLYAITLVWHGKNIALYGRC"
BASE COUNT          205 a          133 c          148 g          199 t
ORIGIN      
        1 cgcaacgccc ataaagcgcg atatttttcc catgccatac cagggtgatt gcataaagtt
       61 tgttttcttt tagctgctga tatacatcag ctctggtaaa cgttgggagt tcagcttttg
      121 gggaaagcaa aacgtaactg ccgatggctc cagacaacgc aatagcggca ccaatcacgc
      181 ctgccaggat aagacgcttt ttgcgttgat tcctggcaga gaacctttct ggcatatcag
      241 acaatggcag ggcgtggtca atatcaccaa tgatgaagcc acaccccgct atatcgatga
      301 cctttccgat aggtaatggt tgatctgctt catagggagt gggctgtcca tcaatccaaa
      361 attcaattgg agataaaagg aaaacacctt cctcacgtat atcaagtaaa aactgattga
      421 ggccttgttc aaggggaaag taaacaatat cgttatcttc tgtgcctatc gttaggtttc
      481 ccatcggaag ttgtaattcc cgattacgca ttgcaccatt gagaagtttt attttatatg
      541 aggataacat ttttaaattt tattcgtcct ggtggttgaa caatgagaaa aaatatggtg
      601 aacttacact gactaagaat agagtagaaa ggaagctgtt gtacagtact atttaatgga
      661 atattcataa ttaatgttta cgtga
//
LOCUS       DAZFAH010000528          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFAH010000528 DAZFAH010000000
VERSION     DAZFAH010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.531"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..215)
                     /locus_tag="ACHJWB_005245"
     CDS             complement(<1..215)
                     /locus_tag="ACHJWB_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316658.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQV"
     gene            complement(212..>683)
                     /locus_tag="ACHJWB_005246"
     CDS             complement(212..>683)
                     /locus_tag="ACHJWB_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4316659.1"
                     /translation="WWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTE
                     MPDFHREDDWWRNGQNLYLDNMEGTGFYRVPLSSAQAGDILLCCFGASVPNHAAIYCG
                     NGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGICNDLAAASACM"
BASE COUNT          149 a          222 c          183 g          129 t
ORIGIN      
        1 acctgaaaaa caccatttcc cccggcccct gccagccgcg gtacaatatg aatcacggcc
       61 ctcccgctca gcggttcatg aagacgggca tacaccgcct ccggcgcggt atcctcaccg
      121 gcaatacgta tctggtacca gccttcgttc atctgaccgc ggaatcccgg cacctgcagc
      181 gacagggcac ggatggcctc cgctgccgtg ttcacataca ggctgaggcg gcggccaaat
      241 cgttgcaaat ccccgtgaag gcagatgcgt gccagtggcg gtgacgccag acagaatgcg
      301 ttcgtcgttg ccatttttcg gaatacctct cccgtttact cagttgttca ggcagatggt
      361 gaagcagctc accgttgcca cagtaaatgg cggcatggtt cggtaccgaa gcaccaaagc
      421 agcacagcag aatatcgccc gcctgtgcag aggacagggg cacccggtaa aagcccgttc
      481 cctccatatt gtccaggtac aggttctggc cgttgcgcca ccagtcatcc tcgcgatgaa
      541 aatccggcat ttcagtcccc gccagatggt acgcatcccg gaacagggtg taacagtccg
      601 tcaccccgtg ttcaaagcgc cgtccggtca ggtgcggaac gcagcggaat ttatgaattt
      661 ccccccggca gaccagccac cag
//
LOCUS       DAZFAH010000529          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFAH010000529 DAZFAH010000000
VERSION     DAZFAH010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.532"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..194)
                     /locus_tag="ACHJWB_005247"
     CDS             complement(<1..194)
                     /locus_tag="ACHJWB_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4316660.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSG"
     gene            complement(194..463)
                     /gene="escS"
                     /locus_tag="ACHJWB_005248"
     CDS             complement(194..463)
                     /gene="escS"
                     /locus_tag="ACHJWB_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO4316661.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(463..>673)
                     /gene="escR"
                     /locus_tag="ACHJWB_005249"
     CDS             complement(463..>673)
                     /gene="escR"
                     /locus_tag="ACHJWB_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO4316662.1"
                     /translation="TMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTIS
                     IPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          244 a          132 c          118 g          179 t
ORIGIN      
        1 attccagatg gcaatttttc cgaaaatgta tagttctcta ctatgatcgg aagcgtaaac
       61 gcaatcataa ttgagttgcg aataaaatta ctgagtaaaa cacctgttga aaatattggt
      121 aggataataa acatccccag cggcctgagt atacaataaa atgatgatac tatgaccgtc
      181 attatctcat tcattagccg ttcaccttcg gaatcatttc aaatattatc gacgagaagt
      241 tgatgattgt tgttcccatc cagtgataag tcagggcaag cgtagcaaac actgctatta
      301 tttttagcaa aaaaggcaat gtttgatcct gtaactgcgt tatagcctgg accagactaa
      361 taataatacc gataacagag gccgctatga ctgtaggcaa tgagaggata aatattatcc
      421 agaacgtttg cacacataat tgaacaaaat atccagtatc catcaattca ccaccaacag
      481 aaattcgaat aatttttgcc agcctccaac aagaatgaat aacaagatct taaaaggaat
      541 cgaaattgtt actggcgata ccatcatcat acccaaggcc aataagatat tggaaatgat
      601 caaatctatc gcaataaagg gtaaatagag caaaaaacct atcttgaatg cagcttcaag
      661 ctgccccatc gtg
//
LOCUS       DAZFAH010000530          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFAH010000530 DAZFAH010000000
VERSION     DAZFAH010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.533"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..301
                     /locus_tag="ACHJWB_005250"
     CDS             <1..301
                     /locus_tag="ACHJWB_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4316663.1"
                     /translation="ALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNG
                     RQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          174 a          147 c          212 g          138 t
ORIGIN      
        1 agccctggca gccggtggtt tttctgccac cacgatgctg ttttctctgg gggccagcat
       61 gatactgggt ggtgtggctc agatgctggc cccgaaggca aaaacaccgg attaccgcgc
      121 aacggataac ggcagacaga acacgtattt ttcctcactg gataacatga ttgcccaggg
      181 gaacccgatg ccggtgcctt acggtgaaat gctggttggc tcccggcgaa tctcccagga
      241 catcagcacc cgtgatgaag gtggtggcgg gaaggtggtg gttatcgggc ggcaggggta
      301 aagcataaaa aaatcccgca gtgtatggag gctgcgggaa cagacaatga agattaacca
      361 caaaggagtt ttgtttttat tgacccgaaa aaactgtaac gctgcgcaat tatcagcgcc
      421 acaggcagtt tgtgaaaatg tgaagaaatt cagaaatttt attccgtcat gacacaggca
      481 ccctccgggg tgcctgtcgt ttttggggca taaacagatt cagacatcag acaggagagg
      541 gggacagagt gggtaaaggt ggcggcaagg cgcacacgcc ggttgaggca aaggacaatc
      601 ttaagtccac gcagatgatg agcgtgattg atgccatcgg tgagggaccg gtggaaggtc
      661 cggtgaaggg g
//
LOCUS       DAZFAH010000531          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFAH010000531 DAZFAH010000000
VERSION     DAZFAH010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.534"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..102)
                     /locus_tag="ACHJWB_005251"
     CDS             complement(<1..102)
                     /locus_tag="ACHJWB_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4316664.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNH"
BASE COUNT          209 a          157 c          130 g          164 t
ORIGIN      
        1 atgattatcc cgacgacaac aaatattaaa atcctgcata ttacccgaca tcaggagttc
       61 aaaaccgtcc ccggcatcat tcatactggc agataaattc attacccagt ttagtgctaa
      121 tcaacaggta cggcttaact tacaggaatt ccccgggagg ggcggactca tcctcagtta
      181 gcggcaaaat cgcatcacag ccaatcgcct gtctcgattc cccatcggcg aacaatgtat
      241 gtggccacaa aatcctggct ggctcagaac gaagagcatc tgagcaaaga ggtcatcaat
      301 ctggtatgaa tatctggcag aaaatatccc ctgaatccac aactgctgtg catatctgct
      361 caaatcagca tcaatcataa accagtaatc acagtgcctg tgattgggtt acaggaataa
      421 gtcccccttc ataaggcata tcatcaatac cggtgaagga aaagaaagca tcagatggcc
      481 tgatagtctg cgtcagtcaa tgacatcagt gctttatccg gaacaatgta tccatgttgg
      541 cgacagagaa agccattgcc atgaacagtt ttgtttatcc ccggaaagtc atttctgtta
      601 caaatttata ttgacaaact tgctttcgat gagaaacata ctatttcaat gaaatgcaga
//
LOCUS       DAZFAH010000532          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFAH010000532 DAZFAH010000000
VERSION     DAZFAH010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.535"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..503)
                     /locus_tag="ACHJWB_005252"
     CDS             complement(<1..503)
                     /locus_tag="ACHJWB_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4316665.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGA"
BASE COUNT          195 a          112 c          130 g          220 t
ORIGIN      
        1 tatgcgccta tgacaaatgc gctcaacata attagataac tttttatatg tttgaggatt
       61 ttgttattga aattctttgc gccaccaatg ctctctttgt atcccgcatt aacttttatg
      121 gcaggtgggg cattgtgtgt atgtgcgtct gttgatgagt ttattacatg attttgtatg
      181 ttaatgtcat tatggatctt gaatcctctt ctcggtacgg ttgcaataag cgagtgggta
      241 gtatcaccaa cagcgcgaaa tccgcgtctg ataatggata tgttctgata taacgtgtta
      301 gtaggcacac gcataccttc ttcttcccag actctggtga aaaaatcttg ttgtgcaacc
      361 acatcaggaa aagcttcaag tagaagtgat aaacaacgac tggctggcgt tgtcagaacg
      421 acgttaagat cggcgttatg taccgatatt aattttcggt gctcaggcca gaactcgata
      481 ttatcgttaa taatccagta catacgtcaa taaacactaa atcaatcgaa atggaaatca
      541 catagtttgc taagtatatt ggtatttacg gcataaatat atattaattt tatatttatc
      601 atgatgattg aaatgaggct ttaatgtcgc aacgtaaaac tttatgtaaa ttaacat
//
LOCUS       DAZFAH010000533          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFAH010000533 DAZFAH010000000
VERSION     DAZFAH010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.536"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>641
                     /locus_tag="ACHJWB_005253"
     CDS             <1..>641
                     /locus_tag="ACHJWB_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4316666.1"
                     /translation="GFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWG
                     LAGVMQEYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSI
                     QAADANILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGA
                     QKDAMNSWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDG"
BASE COUNT          156 a          137 c          207 g          141 t
ORIGIN      
        1 ggatttattc gcccggcagc ggagatggaa acctatatga ttcgcctgaa ctccctttac
       61 aaaggggatc gggcgaaaac tgatgatgtc cgcaggtggg caatacagaa tgcgaaggaa
      121 accacctggg ggctggctgg cgtcatgcag gaatatacgt ccagtctggg ctttggtatg
      181 agtgacaggg aggcccgaaa ttttgtcacc atgcttcagg atcagggtgc cgtgggcggc
      241 tggtcactgt ccgatgcaca gggggcatcc ctgcaactga aacagatgta tgcaagaggc
      301 agtattcagg cagcggatgc aaatattctt gccacctacg gcattaatgc ctaccgcgtg
      361 ctggctgatc gtctgggtgt ggaccagaaa gtggtcagaa aacttggtga aaaagggctg
      421 cttgggccgg acagtattcg cctgttattt cagacgctgg cggaacaggc caggggagca
      481 caaaaggacg caatgaattc ctggagtggc ctgacggcca tgatgggtga cgtctgggat
      541 cagtttgccc gggatgtgat ggacagtggg ccatttgaaa aactgaaggg taatctcagg
      601 ggatttcttt catgggttga cagtgccaaa tctgatggca g
//
LOCUS       DAZFAH010000534          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFAH010000534 DAZFAH010000000
VERSION     DAZFAH010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.537"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..623
                     /locus_tag="ACHJWB_005254"
     CDS             39..623
                     /locus_tag="ACHJWB_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316667.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          177 a          133 c          138 g          181 t
ORIGIN      
        1 cagtagtctg actaatggaa ttttattcaa ggatttttat gtctaaaaaa ttaggttttg
       61 ccctgtctgg tcttatgctg gcaatggttg caggtactgc ttctgctgat atggatggtg
      121 gtcagttaaa tatcagtggt ctggttgttg ataacacctg tgaaacccgc gttgacggcg
      181 gtaataaaga tggtctgatc ctgctgcaaa ctgcaactgt tggtgaaatc gatgctggcg
      241 tgctgaatga caccgttggc gctaaagcca aacctttcag catcaccgtt gactgcagca
      301 aagcaaatcc gaatccaggt agtacagcta aaatgacctt cggttctgtt ttctttggta
      361 atagcaaagg aacgctgaac aacgacatgt ctattaacaa cccgtctgac ggcgtgaata
      421 tcgctctgca caatattgat ggttccacta tcaaacaggt tcaaattaac aaccctggcg
      481 atgtgtatac taaagccctg gatgcaacga caaaatctgc tgtttatgat tttaaagcgt
      541 cttacgttcg tgccgtcgca gaccaaacag caactgctgg ttatgtaaaa actaacactg
      601 catacaccat tacttatcag taagtattc
//
LOCUS       DAZFAH010000535          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFAH010000535 DAZFAH010000000
VERSION     DAZFAH010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.538"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>625
                     /gene="sepL"
                     /locus_tag="ACHJWB_005255"
     CDS             <1..>625
                     /gene="sepL"
                     /locus_tag="ACHJWB_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4316668.1"
                     /translation="NELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQ
                     ELLTLTDSDKLKELSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELK
                     KKYAKLLLELLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDW
                     FMEVKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRI"
BASE COUNT          201 a           98 c          153 g          173 t
ORIGIN      
        1 aaaatgaatt agcgatgatt accagttcgt cgctttctga aacgattgaa gggctaagcc
       61 tagggtatcg caaaggtagc gccaggaaag aagaggaagg tacgacaacg gagaagctgc
      121 tcaatgatat gcaagagctg cttactctga ccgatagcga taaactaaaa gaactatcat
      181 taaagaacag tgggctgctg gaacagcatg atcctaactt ggctatgttt ggtaatatgc
      241 caaaagggga aattgtcgcg ctcatatctt ctctattgca atcaaagttt gttaagattg
      301 aattaaaaaa gaaatatgcc aaattattat tagaattatt aggcgaagat gattgggagt
      361 tggctctgct ttcctggtta ggggtgggtg agttaaatca ggaaggtatc cagaagatca
      421 agaagcttta tgaaaaggct aaggacgagg actctgaaaa cggagcctct ttacttgact
      481 ggtttatgga ggtcaaggat cttccagagc gtgagaagca cttaaaggtt attattaggg
      541 cgctatcgtt cgatctttct tatatgtctt cttttgaaga caaggtaaaa acatcttcaa
      601 ttattagcga tttatgccgg ataat
//
LOCUS       DAZFAH010000536          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFAH010000536 DAZFAH010000000
VERSION     DAZFAH010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.539"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..275
                     /locus_tag="ACHJWB_005256"
                     /pseudo
     CDS             123..275
                     /locus_tag="ACHJWB_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(300..>623)
                     /locus_tag="ACHJWB_005257"
     CDS             complement(300..>623)
                     /locus_tag="ACHJWB_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316669.1"
                     /translation="QFIIYTLTSWSILITLNDINLALSFILVINNTYVNINIGAKRIR
                     DTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKM
                     SLSAH"
BASE COUNT          197 a          115 c          114 g          197 t
ORIGIN      
        1 aaaaaaatat ttaaaaaaga agtaaacttc acattttttc attacatctc tgaagagaac
       61 ctcgttaatg attggtcttg ctcattgtta ataacaaaat gttgaattta ccgattagga
      121 aactttttcc tacgcggatt gcctccagct atggcatgaa ctttgagttt atgccagaac
      181 tgaagtgggg cttcggctac cctggcgcga ttatctttat gatcctcgcg ggcctgacac
      241 cgtatctgta ctttaagcgt aagaactggc tgtaactata cgatgtcgct gtgagtaagt
      301 tagtgtgctg acagcgacat tttagccgag ggaatcgcag ataacataat tgttgtgaat
      361 aatatccatg ccacgttgag ttggacgctt aatgactcac atctcacccc ttcaattgct
      421 gcagagataa tagaaaaaag tgtcaagccc aatgaaatca atacaaagta gattgcctta
      481 catccagtat ctcttattct ttttgctcca atattgatat ttacatatgt attattaata
      541 accagaataa aactcaaagc caggttgata tcattcaaag ttattaatat tgaccatgat
      601 gttaatgtat agattatgaa ctg
//
LOCUS       DAZFAH010000537          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFAH010000537 DAZFAH010000000
VERSION     DAZFAH010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.540"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>623
                     /locus_tag="ACHJWB_005258"
     CDS             <1..>623
                     /locus_tag="ACHJWB_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316670.1"
                     /translation="INSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVY
                     DLTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVG
                     KSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGLWELIDNSQLADEMAEMKTSITET
                     RNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNG"
BASE COUNT          173 a          147 c          181 g          122 t
ORIGIN      
        1 attaacagtt atggccagca gggggaaccg gccaccacca cgttcaggat taatgcacct
       61 gcggtacccg ccacgattga gctgacaccg ggctattttc agataacagc ggtcccgcgt
      121 cttgcggtgt atgacctgac ggtacagttt gagttctggt tttcggaggc aaaaatcgca
      181 gacacatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca gtggagtgta
      241 tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag cgtcaacctg
      301 gtggggaaat ctgcgtttgt ggaagccagt ggccgggcca gcaatgatgc agaagggtat
      361 ctggactttt tcagaggaga aataggaaaa acgcatctgg ctcaggggtt gtgggaactg
      421 atagataaca gccagcttgc ggatgagatg gcggagatga agaccagcat cacggaaacc
      481 cgcaatgaaa tcacacagac ggtcaacaaa acgctggagg accagagcgc caccatacag
      541 cagatacagc gcgtgcagaa ggacacaaat gatgaccttg ctgcacttta catgctgaag
      601 gtacagaaaa caaaaaatgg cat
//
LOCUS       DAZFAH010000538          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFAH010000538 DAZFAH010000000
VERSION     DAZFAH010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.541"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..140)
                     /locus_tag="ACHJWB_005259"
     CDS             complement(<1..140)
                     /locus_tag="ACHJWB_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316671.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..546)
                     /locus_tag="ACHJWB_005260"
     CDS             complement(121..546)
                     /locus_tag="ACHJWB_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4316672.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          138 a          204 c          139 g          140 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggccagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
      601 gtcgcttctg ctttcccggt t
//
LOCUS       DAZFAH010000539          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFAH010000539 DAZFAH010000000
VERSION     DAZFAH010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.542"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHJWB_005261"
     CDS             complement(<1..173)
                     /locus_tag="ACHJWB_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316673.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHJWB_005262"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHJWB_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          135 a          196 c          134 g          147 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg ataccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgtccttcct ctgcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacatc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccggctc agtcgcttct
      601 gttttcccgg tt
//
LOCUS       DAZFAH010000540          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFAH010000540 DAZFAH010000000
VERSION     DAZFAH010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.543"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..357
                     /locus_tag="ACHJWB_005263"
     CDS             <1..357
                     /locus_tag="ACHJWB_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4316674.1"
                     /translation="DKKARELEQANATITDMQVRQRDVAALDAKYSRELADARAENET
                     LRADVAAGRKRLRINATCSGTVREATGTSGVDNATGPRLADTAERDYFILRERLMTMQ
                     KQLEGAQDYIRTQCLN"
BASE COUNT          155 a          158 c          178 g          121 t
ORIGIN      
        1 gataaaaaag ccagagagct ggaacaggcg aatgccacca ttactgacat gcaggtgcgc
       61 cagcgtgatg ttgctgcgct cgatgcaaaa tactcgaggg agttagccga tgcgagagct
      121 gaaaatgaaa ctctgcgtgc tgatgttgcc gctggtcgta agcgcctgcg gatcaacgcc
      181 acctgctccg gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa tgcaaccggc
      241 ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg gttgatgacc
      301 atgcagaagc agctggaagg ggcacaggac tatatccgca ctcagtgcct gaactaagtt
      361 ttgctgatgc gccgtatcgt cgccgtattc ctgcattaac agagaccgca gcccgacagg
      421 gagactcctc tgcgagagtg tgcggggata atcaaaaacg atacacaccg gggtttaccg
      481 cgtaaacgga gcgcggcgtt ctcccctcat ggtcgcccgt ccggtgcgat ggtggaagaa
      541 actggaatct gttcaataaa aaaactgccg tgttggagtc acagcagtaa tgtactgatt
      601 gggtagaaga tt
//
LOCUS       DAZFAH010000541          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFAH010000541 DAZFAH010000000
VERSION     DAZFAH010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.544"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..196
                     /locus_tag="ACHJWB_005264"
     CDS             <1..196
                     /locus_tag="ACHJWB_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316675.1"
                     /translation="EMWNCKKIIDIKPLADIKSVKRIYIMGCSVNMDDNNLCDYFKSR
                     GFTKIRYEPNRSDTLFEAIR"
BASE COUNT          186 a          102 c          165 g          152 t
ORIGIN      
        1 tgaaatgtgg aattgtaaaa aaatcataga tattaaacca ctggctgata ttaaaagtgt
       61 aaaaagaatt tatatcatgg gatgcagtgt aaatatggat gataataatc tttgtgacta
      121 ttttaaatcg cgaggtttta ctaaaataag atatgaacca aataggagtg atacgctatt
      181 tgaagcaatt agatagggtt tgttccaggg aaagatcacg gtgtgccgta ggctcaaaga
      241 tgcaaaacgg gacgttttgc atgtaataag cataagcttg tgaagagatg gtggcaggcc
      301 caccgggatc tgctgtcgga agtcggtccc aaacaacccc ggctgctgtg gtaaagagcc
      361 atggtagtga gcgcaggggg aacgatcaga cgagatctga tcctatgtga gatacagtga
      421 gatgagtaaa cccgaacctg tgttgaaacg tcgttagtcc taagtgacat caaaaccgga
      481 ggtttcgtga taccgccggg attaatttac gggtgaattc cgaatgctgc gtaaatggtg
      541 tccggcgtat agcaggcatg acgctgtatc aggctctttg caggaactac gggaaccggt
      601 cgtcg
//
LOCUS       DAZFAH010000542          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFAH010000542 DAZFAH010000000
VERSION     DAZFAH010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.545"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..604
                     /locus_tag="ACHJWB_005265"
                     /pseudo
     CDS             150..604
                     /locus_tag="ACHJWB_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
BASE COUNT          181 a           93 c          151 g          179 t
ORIGIN      
        1 gcgtgctgac gggacgtggg aaaatattgg tgggatgaag taacccgacg gattcacaaa
       61 aaccggagtc cggctccggt ttttgttgtc atgtcatggt gatgtttgtt aagaaagtaa
      121 agattgattc attttgaagg ttgaaatgta tgctatcacc atcttctgta aatttggggt
      181 gttcatggaa ttctttaacc agaaacctga cttctcctga cagtcgtatt ttatcctctg
      241 taagggatgc agctgctagc tggtaatgtt attggcaaag ggggtaacgc tgtagtatat
      301 gaagatgctg aggatgcaac aaaagtcctg aagatgttta ctacatctca aagcaatgaa
      361 gaggtaacaa acgaagttcg ttgcttcaac caatgttatg gtgccgggag tgcagaaaaa
      421 atatatggcg ataatggcga tattattggt attagaatga ataaaataaa cggggagtcg
      481 ctttttaata tttcgtcatt gccagtacag gctgagcatg ctatttacga tatgtttgac
      541 aggctggagc aaaaaggaat tctttttatc gatacaacag agacaaatgt cttatatgac
      601 cgtg
//
LOCUS       DAZFAH010000543          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFAH010000543 DAZFAH010000000
VERSION     DAZFAH010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.546"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..>599
                     /locus_tag="ACHJWB_005266"
     CDS             84..>599
                     /locus_tag="ACHJWB_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4316676.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          175 a          116 c          142 g          166 t
ORIGIN      
        1 aatggcggtc aattattgtt attattaagt ctaatgtcat ttaaaggcat tatcatattt
       61 acgaagagat caggatgatg acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg
      121 tatgtgcaac gtccagcgtt atggcagctg atgataatgc tatcacggat ggcaaagtaa
      181 catttaatgg taaagttatt gctccagctt gtaccctggt agctgcgacg aaagattcag
      241 tagtcacatt gccaaatgtc agcgccacca agttgcaaac taatggagca gtctctggcg
      301 ttaaaactga tgtaccaatt gccttagaag gctgtgatgt tactgtaact aaaaatgcga
      361 cgtttacctt tagtggtacg gctgacggcg tgcaacctac agcatttgcc aaccaggcta
      421 ccaccgatgc agcgacgaat gttgctctac aaatgtatct gccagatggt tctacatcgg
      481 taactcccgg taccgaaacg tcgaatattc agttagcgga ttctgctgag caaacagtga
      541 cctttaaagt tgattatatc gctacgggga aagcgacttc tggtaatgtg aatgcggta
//
LOCUS       DAZFAH010000544          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFAH010000544 DAZFAH010000000
VERSION     DAZFAH010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.547"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>598)
                     /locus_tag="ACHJWB_005267"
     CDS             complement(<1..>598)
                     /locus_tag="ACHJWB_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4316677.1"
                     /translation="KISMPEDVFLVSLDKYNIDFALCSCGSAVEVDHDQNPIPDEDQV
                     TQHDNNERMLRLVRQHAKRIGAFMWIKPRLESCDQDFEDMIASNRDIIYGIKVHPYHS
                     KMAFNSEKVQYYIRLAQKYDLPVVSHTSNDYESSPQLVYEMAQKYPDVNFVMCHMGLA
                     TDNQEAIELIAKLPNLYGDTAWVRADMVYQAIKICGSDK"
BASE COUNT          178 a          132 c          108 g          180 t
ORIGIN      
        1 cttatcacta ccacagattt ttatcgcctg gtaaaccata tccgccctaa cccacgcagt
       61 atcgccatag agattaggaa gttttgcaat caattcgatt gcttcctgat tatcagtcgc
      121 gagccccata tgacacataa caaaattcac gtctggatac ttttgtgcca tctcataaac
      181 taactgtggg ctggattcat aatcattaga agtatggcta actaccggga gatcatattt
      241 ttgagcaagg cgtatataat actgaacttt ttctgagttg aaagccattt ttgaatgata
      301 agggtgaact ttgatgccat aaatgatatc tcgattgctg gcaatcatat cttcaaagtc
      361 ttgatcacaa ctttccagac gaggcttaat ccacataaac gcgccaattc gctttgcatg
      421 ttgacgaaca agtctgagca tacgttcatt gttatcatgc tgtgtaactt gatcttcatc
      481 cggaatcggg ttctggtcat gatcaacttc aacggcagat ccacaggaac ataacgcaaa
      541 atcaatatta tacttgtcca gagataccag aaaaacatct tctggcatgc tgattttc
//
LOCUS       DAZFAH010000545          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFAH010000545 DAZFAH010000000
VERSION     DAZFAH010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.548"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>586
                     /locus_tag="ACHJWB_005268"
     CDS             <1..>586
                     /locus_tag="ACHJWB_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4316678.1"
                     /translation="KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTD
                     EKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNK
                     AFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCV
                     AVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFAT"
BASE COUNT          201 a          106 c          139 g          140 t
ORIGIN      
        1 aaagacgtca ttgcaattga tggaaaaacg ctccggcact cttatgacaa gagtcgccgc
       61 aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt catcggacag
      121 atcaggacgg atgagaaatc taatgagatt acagctatcc cagaacttct taacatgctg
      181 gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga tattgcagag
      241 aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca ggggcggcta
      301 aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt
      361 tacgcaatta gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat
      421 gtccctgatg aacttattga tttcacgttt gaatggaaag ggctgaagaa attatgcgtg
      481 gcagtctcct ttcggtccat aatagcagaa caaaagaaag agccagaaat gacggtcaga
      541 tattatatca gttctgctga tttaaccgct gagaaattcg ccacag
//
LOCUS       DAZFAH010000546          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFAH010000546 DAZFAH010000000
VERSION     DAZFAH010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.549"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..501)
                     /locus_tag="ACHJWB_005269"
     CDS             complement(<1..501)
                     /locus_tag="ACHJWB_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4316679.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFN"
BASE COUNT          196 a          122 c          130 g          134 t
ORIGIN      
        1 gttaaatgaa accagttttc cgcgaatatg agctggagcc agttccgcaa tatacattgg
       61 tgaaagcatt gaggctaaac caacgccaat accgccaata atacgataaa taacaaattc
      121 cgggacataa cctgccagat aaataggtac tgtgttgtcc gggtttatag aggtaaaacc
      181 aagttctggc caggcagaac ctacaccaga aataaaaaac aggacagcag caatcttaag
      241 tgaatcacga cggccgaagc ggttactgca ataaccaccg agggcaccgc cgatgatgca
      301 accaatcaga gcgctggcta cgcaaaatcc taacagggag ttggcagcgg attcacttaa
      361 gttttgtgga gcaacaaaga cggtattgag tgactcaaca gtaccggaaa taacggcggt
      421 gtcgtagcca aataataaac cacctaatgt agcgactaag gtaatcgaaa atatataact
      481 ggaattatac tgggtattca ttcagacctg ccttagacca ttctgatctt ttatcagtgc
      541 attgatgttg gaattgtcag gcagttaagg caattatcat tt
//
LOCUS       DAZFAH010000547          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFAH010000547 DAZFAH010000000
VERSION     DAZFAH010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.550"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..463
                     /locus_tag="ACHJWB_005270"
     CDS             <1..463
                     /locus_tag="ACHJWB_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4316680.1"
                     /translation="QLRRLQKLPGAPVSNKRGQLDLDAWRDFYISYLRRSKNDVPDGD
                     SEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDS
                     IPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            435..>580
                     /locus_tag="ACHJWB_005271"
     CDS             435..>580
                     /locus_tag="ACHJWB_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316681.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEG"
BASE COUNT          170 a          121 c          145 g          144 t
ORIGIN      
        1 ccagcttcgc agattgcaga aattaccggg tgccccggtc tcgaataagc gagggcaact
       61 ggatctggat gcctggcgcg atttttacat atcgtatctg agaagaagta aaaacgatgt
      121 gcctgatggc gatagcgaag aagactatga agagaaattg cttattgcca gatgggaact
      181 gacagcagaa caggctgtta cacagcagtt aaaaaatgag gtgtcaaaag gaaaactgat
      241 tgacaccggg ttctgtattt ttgccctcag taagctggca atggcgttat ccagtacgct
      301 tgattccatc cctttatcca tgcagcgaca gtttcctgat ttaacaccgc gccatcttga
      361 ccatctgaaa acccttattg ctaagggggc aaatcagtgt gcgcgggcag gggataaatt
      421 accggattta cttgatgaat atatcagagc aacaactgaa taatatgatg agcgctgtca
      481 caactgcatt acagcccctg ataagggcat tgccggtgac gccagttgaa tgggctgatc
      541 aaaattatta tctgcctaaa gaatcttcat atggtgaggg
//
LOCUS       DAZFAH010000548          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFAH010000548 DAZFAH010000000
VERSION     DAZFAH010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.551"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..438
                     /locus_tag="ACHJWB_005272"
     CDS             <1..438
                     /locus_tag="ACHJWB_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4316682.1"
                     /translation="PGAPVSNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEDDYEEK
                     LLIARWKLIEEQAIAQQLKNQASEGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQ
                     FLDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            410..>567
                     /locus_tag="ACHJWB_005273"
     CDS             410..>567
                     /locus_tag="ACHJWB_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316683.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGDGEWKT"
BASE COUNT          160 a          121 c          145 g          141 t
ORIGIN      
        1 ccgggtgctc cggtctcgaa taagcgagga caactggatc tggatgcctg gcgtgatttt
       61 tacatatcgt atctgaggag aagtaaaaac gatgtgcctg atggcgatag cgaagacgac
      121 tatgaagaaa agttactcat tgccagatgg aagttgatcg aagaacaggc aatcgcccag
      181 caattaaaaa atcaggcgtc cgaaggaaag cttattgaca ccggattctg tatttttgcc
      241 ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcag
      301 cgacagtttc ttgatttaac accgcgccat cttgaccatc tgaaaaccct tattgcgaag
      361 ggggcaaatc agtgtgcgcg ggcaggggat aaattaccgg atttactcga tgaatatatc
      421 agagcaacaa ctgaataata tgatggctgc cgtttcggtt gcgctgcagc ctctggtcag
      481 ggttgtaccg atgacggcag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc
      541 ctcatacggt gacggcgaat ggaaaac
//
LOCUS       DAZFAH010000549          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFAH010000549 DAZFAH010000000
VERSION     DAZFAH010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.552"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>561
                     /locus_tag="ACHJWB_005274"
     CDS             <1..>561
                     /locus_tag="ACHJWB_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4316684.1"
                     /translation="NEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSH
                     SEMIEDNKKDLTVNDHGLTCPSTTDCS"
BASE COUNT          172 a          100 c          151 g          138 t
ORIGIN      
        1 aatgaggaga acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat
       61 aaggagtcgg ggctgtacta caaccggaac cggtactacg atccgttgca ggggcggtat
      121 atcactcagg acccgatagg gctggcgggg ggatggagtc tgtatgcgta tccgctgaat
      181 ccggtgaatg gtattgatcc attagggtta agtcccgcag atgtagcgct aataagaaga
      241 aaagatcaac taaaccatca aagagcatgg gatatattat ctgatactta tgaagatatg
      301 aagagattaa atttaggtgg gactgatcaa tttttccatt gtatggcatt ttgtcgagtg
      361 tctaaattaa atgacgctgg tgttagccga tcggcgaaag ggctgggtta tgaaaaagag
      421 attagagatt acgggttaaa tctgttcggt atgtacggca gaaaagtaaa gctatcccat
      481 tctgaaatga ttgaagataa taaaaaagac ttgactgtaa atgaccatgg gttgacatgt
      541 ccatcaacaa cagattgctc a
//
LOCUS       DAZFAH010000550          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFAH010000550 DAZFAH010000000
VERSION     DAZFAH010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.553"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            255..>560
                     /gene="tccP"
                     /locus_tag="ACHJWB_005275"
                     /gene_synonym="espF(U)"
     CDS             255..>560
                     /gene="tccP"
                     /locus_tag="ACHJWB_005275"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO4316685.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     "
BASE COUNT          178 a          127 c           93 g          162 t
ORIGIN      
        1 tttggcattt tgggaagatc agcgggacat aagagacata caacacagga ggaatggctg
       61 ttttttaata gtgctttgaa ttccgcatga aacctttctt agcagtcgaa aagcaaaaca
      121 tacgttttct ttttaacaat aattgatgag tattgcatcg agtgtcattt atacatcgcc
      181 gcatttattt gccactataa aaaggctgat tacatatcta tcggagaact aaaattactt
      241 ataaggtaat ctgcatgatt aacaatgttt cttcactttt tccaaccgtc aaccgcaata
      301 ttacagctgt atataaaaaa agcagcttct ctgtatcacc acagaaaatc acattaaatc
      361 ctgtaaaaat cagctcacct ttttcaccaa gcagtagctc catcagcgca acaactctct
      421 ttcgagcccc aaacgcccat tcggcatcat ttcatcgaca gtctactgct gaaagttcgt
      481 tacatcaaca acttcctaat gtgaggcagc gcctgataca acatcttgca gagcatggca
      541 ttaaacctgc ccggagtatg
//
LOCUS       DAZFAH010000551          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFAH010000551 DAZFAH010000000
VERSION     DAZFAH010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.554"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>554
                     /locus_tag="ACHJWB_005276"
     CDS             <1..>554
                     /locus_tag="ACHJWB_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO4316686.1"
                     /translation="SKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENV
                     HKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEAC
                     MLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLF
                     MDPKRMIEACDENTIGVVPTFGVT"
BASE COUNT          142 a          139 c          149 g          124 t
ORIGIN      
        1 tcaaaacgat ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat
       61 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
      121 gacgaaaacg tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa
      181 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
      241 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      301 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      361 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      421 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      481 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact
      541 ttcggcgtga ccta
//
LOCUS       DAZFAH010000552          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFAH010000552 DAZFAH010000000
VERSION     DAZFAH010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.555"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>551
                     /locus_tag="ACHJWB_005277"
     CDS             <1..>551
                     /locus_tag="ACHJWB_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316687.1"
                     /translation="EQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQR
                     KSELLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYN
                     IRHSLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGG
                     EAGVTDNAYRFWRRCRSDGLGNRV"
BASE COUNT          123 a          128 c          182 g          118 t
ORIGIN      
        1 gaacaactga tttttaaact gctggcggca gaagaagagt atgagcgaac cggcagtgaa
       61 gagaccctga aagcggtggt gaacaccgat atcggacgac cctatctgcc ccgttcagcc
      121 acggaacagc gtaaaagtga actgcttgaa cagcgtgccg agccgtttcc ccggcgatct
      181 gtgccggatg gtgtgcgttt tattgaggca acggttgacg tacagggcgg taaaaatcgc
      241 cgttttgttg tgcagatcac cggatacgga gagcaggggg aacgctggat tgttgatcgc
      301 tacaacatcc ggcattcact gcgctgcagt cccaacggtg aaagtctgcc ggttgatccg
      361 gcggcatatc cggaggactg ggatttgttg ctgacggatg tgttccataa aacatggccg
      421 ctggcttctg atccggatgt gcgcatgcgt ctgatggcca tggcggtgga tacgggaggg
      481 gaagccgggg tgacagataa cgcctatcgt ttctggcgtc gttgccggag tgacggactg
      541 ggcaacaggg t
//
LOCUS       DAZFAH010000553          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFAH010000553 DAZFAH010000000
VERSION     DAZFAH010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.556"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>549
                     /locus_tag="ACHJWB_005278"
     CDS             <1..>549
                     /locus_tag="ACHJWB_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316688.1"
                     /translation="ARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQ
                     LWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSG
                     TIHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSW
                     KNRAEVELATLTWVDWYNNRRLL"
BASE COUNT          128 a          133 c          167 g          121 t
ORIGIN      
        1 gcgcgcctca tggcggttat gggacttgcc ggtgttctcc ggggtaaaaa ggtccgcact
       61 accgtcagcc ggaaaaccgt tgccacaggt gaccgcgtaa accgtcagtt cgtggcagaa
      121 cgtcctgacc agctgtgggt ggctgatttt acttacgtca gcacatggca gggcttcgtc
      181 tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tggggtggcg ggtctcatcg
      241 tctatggaaa cgacattcgt gctggatgcg ctggagcagg cgttgtgggc ccgtcgtccg
      301 tctggcacca tccatcacag cgataaaggc tctcagtatg tgtcactggc ctatacggag
      361 cgactaaaag aagcaaaact gctggcatca acagggagta caggcgactc gtatgacaac
      421 gcgatggctg agagcatcaa tggtctttac aaagcggagg taatacaccg taagagctgg
      481 aaaaaccgtg cagaagtgga actggccaca ctcacgtggg tggactggta taacaatcga
      541 cgattgctg
//
LOCUS       DAZFAH010000554          547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFAH010000554 DAZFAH010000000
VERSION     DAZFAH010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.557"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..541
                     /gene="pagP"
                     /locus_tag="ACHJWB_005279"
     CDS             <1..541
                     /gene="pagP"
                     /locus_tag="ACHJWB_005279"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4316689.1"
                     /translation="AIFSFVFIQLISVGKVFANADEWMTTFRENIAQAWQQPEHYDLY
                     IPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPI
                     AGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTY
                     IPGTYNNGNVYFAWMRFQF"
BASE COUNT          139 a          120 c          138 g          150 t
ORIGIN      
        1 cgctatcttt tcctttgttt ttattcagtt aatcagcgtt ggtaaagttt ttgctaacgc
       61 agatgagtgg atgacaacgt ttagagaaaa tattgcacaa gcctggcaac agcctgaaca
      121 ttatgattta tatattcctg ccatcacctg gcatgcacgt ttcgcttacg acaaagaaaa
      181 aaccgatcgc tataacgagc gaccgtgggg tggcggtttt ggtcagtcgc gttgggatga
      241 aaaaggaaac tggcatggcc tgtatgccat ggcatttaag gactcgtgga acaaatggga
      301 accgattgcc ggatacggat gggaaagtac ctggcgaccg ctggcggatg aaaattttca
      361 tttaggtctg ggattcaccg ctggcgtaac ggcacgcgat aactggaatt acatccctct
      421 cccggttcta ctgccattgg cctccgtagg ttatggccca gtgacttttc agatgaccta
      481 cattccgggt acctacaaca atggcaatgt gtactttgcc tggatgcgct ttcagttttg
      541 agacaaa
//
LOCUS       DAZFAH010000555          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFAH010000555 DAZFAH010000000
VERSION     DAZFAH010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.558"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>544)
                     /locus_tag="ACHJWB_005280"
     CDS             complement(<1..>544)
                     /locus_tag="ACHJWB_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316690.1"
                     /translation="IVQILPMLFLLTQLDFSEGTSDLVEMLMLLALTAAIVTIGILPG
                     AIHLNERRNGDKFTGFIVVLICIPVVITVFSMWYHPIPNMIINMVMSLSGISDWRTHQ
                     YYIENKTHSHSMFNGLIWNTRYYTEIPERFFITGVSTFTLGDIKLVCPTEITEARKES
                     LKFTINDINEYEQKGKQLKAV"
BASE COUNT          171 a          106 c           91 g          176 t
ORIGIN      
        1 tactgccttt aactgtttcc ctttttgctc atactcattg atatcattaa tggtaaattt
       61 caaactctct tttcttgctt ctgttatttc agttgggcat accagcttta tatctcctaa
      121 ggtaaatgta ctgaccccgg tgatgaaaaa tctctcgggg atttctgtat agtaccgagt
      181 attccagatt aagccattaa acatactatg agaatgagtt ttattttcga tataatactg
      241 atgcgttctc cagtcactaa tgccagagag actcattacc atattaatga tcatatttgg
      301 gatagggtga taccacatac tgaatacagt aattacaact ggtatgcata ttaaaacaac
      361 tatgaatcca gtgaatttat caccatttct gcgctcatta agatgtatgg caccaggcag
      421 aatgccaata gtaacaatag ctgctgttaa ggcaaggagc attagcattt caaccaaatc
      481 acttgtcccc tcactaaaat caagttgggt aagcagaaat aacataggta atatttgaac
      541 gatc
//
LOCUS       DAZFAH010000556          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFAH010000556 DAZFAH010000000
VERSION     DAZFAH010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.559"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>544
                     /gene="nleB"
                     /locus_tag="ACHJWB_005281"
     CDS             <1..>544
                     /gene="nleB"
                     /locus_tag="ACHJWB_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO4316691.1"
                     /translation="SDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIES
                     YAEKREHTWFDFFRNLAILKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVL
                     YAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGI
                     KRHFNYSSLHNYNAFCDFIE"
BASE COUNT          175 a           80 c          116 g          173 t
ORIGIN      
        1 tatctgatat ttaccttgaa aatatccata aacaagaatc ataccctgcc agtgagaggg
       61 ataattatct gttaggctta ttaagagaag agttaaaaaa tatcccagaa ggtaaggact
      121 ctttgattga gtcatatgca gaaaaaagag aacatacttg gtttgatttt ttcaggaatt
      181 tggccatatt gaaggctgga agtttgttta cagagacggg aaaaactgga tgccataaca
      241 tatcgccctg tagcggatgt atatatcttg atgccgacat gattattacc gataaattag
      301 gagtcctgta tgctcctgat ggtatcgctg tgcatgtaga ttgtaatgat gagataaaaa
      361 gtcttgaaaa tggtgcgata gttgtcaatc gtagtaatca tccagcatta cttgcaggcc
      421 tcgatattat gaagagtaaa gttgacgctc atccatatta tgatggtcta ggaaagggta
      481 tcaagcggca ttttaactat tcatcgttac acaattataa tgctttttgt gattttattg
      541 aatt
//
LOCUS       DAZFAH010000557          542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFAH010000557 DAZFAH010000000
VERSION     DAZFAH010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.560"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>542
                     /locus_tag="ACHJWB_005282"
     CDS             <1..>542
                     /locus_tag="ACHJWB_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4316692.1"
                     /translation="ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     LASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTY
                     PLSPVNSMDPLGLYEFKSKN"
BASE COUNT          128 a          140 c          174 g          100 t
ORIGIN      
        1 gcggatgccc ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg
       61 ctggtgcaga tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa
      121 agccgccgct ggctggcatc gtgcggcctg accgtggagc agatgcaaaa ccagatggac
      181 ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg
      241 ctggcgctca tcagcacgga aggggcaaca gcgtggtgcg cagaatatga tgaatggggc
      301 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccggct gccggggcag
      361 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      421 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaacct gtatacatat
      481 ccgctgagcc cggtgaatag catggatcca ttaggattat atgaatttaa atcaaaaaat
      541 at
//
LOCUS       DAZFAH010000558          541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFAH010000558 DAZFAH010000000
VERSION     DAZFAH010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.561"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..>541
                     /gene="rcsA"
                     /locus_tag="ACHJWB_005283"
     CDS             271..>541
                     /gene="rcsA"
                     /locus_tag="ACHJWB_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4316693.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVH"
BASE COUNT          164 a          106 c          105 g          166 t
ORIGIN      
        1 ttatcctaag gattatccga aaaataatac ctacgaacat cttccaggat actcctgcag
       61 cgaaatattt gttttaagct cactcacata tcgcaacatt tactttactt taagacaatt
      121 ccaggcaaat tatacaacac tttacgggat agtaagtccg cctgaaaaat cgcgagagtg
      181 gcgcattagg tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa
      241 ttatcgttac gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt
      301 tacacccgac taggtttaac cgggtatctg ttgagtagag gggttaaaaa aagagaaatc
      361 aacgacattg aaaccgttga tgaccttgcc atagcttgtg attcacagcg cccttcagtg
      421 gtgtttatta atgaggactg tttcatccat gatgcttcta acagtcagca catcaagcac
      481 atcattaatc aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat
      541 t
//
LOCUS       DAZFAH010000559          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFAH010000559 DAZFAH010000000
VERSION     DAZFAH010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.562"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>536
                     /locus_tag="ACHJWB_005284"
     CDS             <1..>536
                     /locus_tag="ACHJWB_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine acetyltransferase"
                     /protein_id="HFO4316694.1"
                     /translation="INSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVY
                     DPTVQFEFWFSETKIADISQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVG
                     KSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSITET
                     RNEITQTVSKTLEDQSAT"
BASE COUNT          141 a          128 c          157 g          110 t
ORIGIN      
        1 attaacagtt atggccagca gggcgaaccg gccaccacca cgttcaggat taatgcacct
       61 gcggtacccg ccacgattga gctgacaccg ggctattttc agataacagc ggtcccgcgt
      121 cttgcggtgt atgacccgac ggtacagttt gagttctggt tttcggagac aaaaatcgca
      181 gacatatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca gtggagtgta
      241 tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag cgtcaacctg
      301 gtggggaaat ctgcgtttgt ggaagtcagc gggcagccca gcaatgatgg tgaagggtat
      361 ctggaatttt tccgggaaaa aataggaaaa ctgcatctgg ctcaggggtt gtgggaactg
      421 atagataaca gccagcttgc agatgagatg gcggagatga agaccagcat cacggaaacc
      481 cgcaatgaaa tcacacagac ggtcagtaaa acgctggaag accagagcgc caccat
//
LOCUS       DAZFAH010000560          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFAH010000560 DAZFAH010000000
VERSION     DAZFAH010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.563"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>536
                     /locus_tag="ACHJWB_005285"
     CDS             <1..>536
                     /locus_tag="ACHJWB_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316695.1"
                     /translation="GAWDDRENARLGLAAATLQSDMEKAGELAARDRAERDASQLKYT
                     GEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQ
                     SGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEE
                     AAQRRQLSAQEKSLLAHK"
BASE COUNT          142 a          132 c          186 g           76 t
ORIGIN      
        1 ggtgcatggg atgaccggga aaatgcccgt ctggggctgg cagcggccac gctgcagtcg
       61 gatatggaaa aagccggcga actggccgcc agggaccggg ccgaacggga cgcatcacag
      121 ctgaagtata ccggagaggc gcagaaggcg tatgagcgtc tgctgacgcc gctggagaaa
      181 tataccgccc gtcaggaaga actgaataag gccctgaaag acgggaaaat cctgcaggca
      241 gattacaaca cgctgatggc ggcggcgaaa aaggattatg aagcgacgct gaaaaagccg
      301 aaacagtccg gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc
      361 ctgctgacgc ttcaggctga actccggacg ctggagaagc atgccggagc aaatgagaaa
      421 atcagccagc agcgccggga tttgtggaag gcggagagtc agttcgcggt actggaggag
      481 gcggcgcaac gtcgccagct gtctgcacag gagaaatccc tgctggcgca taaaga
//
LOCUS       DAZFAH010000561          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFAH010000561 DAZFAH010000000
VERSION     DAZFAH010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.564"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>536
                     /locus_tag="ACHJWB_005286"
     CDS             <1..>536
                     /locus_tag="ACHJWB_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316696.1"
                     /translation="GAWDDRENARLGLAAATLQSDMEKAGELAARDRAERDASQLKYT
                     GEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYESTLKKPKS
                     SGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWKAENQYAVLKE
                     AATKRQLSEQEKSLLTHE"
BASE COUNT          157 a          129 c          174 g           76 t
ORIGIN      
        1 ggtgcatggg atgaccggga aaatgcccgt ctggggctgg cagcggccac gctgcagtcg
       61 gatatggaaa aagccggtga actggccgcc agggaccggg ccgaacggga cgcatcacag
      121 ctgaagtata ccggagaggc gcagaaggcg tatgagcgtc tgctgacgcc gctggagaaa
      181 tataccgccc gtcaggaaga actgaataag gccctgaaag acgggaaaat cctgcaggca
      241 gattacaaca cgctgatggc ggcagcgaaa aaggattatg aatcgacgct gaaaaagccg
      301 aagtcgtcag gagtcaaagt gtcagccggt gagcgtcagg aagaccaggc gcatgctgcc
      361 ctgctggcgc ttgaaaccga gctccggacg ctggaaaaac acagcggtgc gaatgagaaa
      421 atcagccagc agcgtcgcga tttatggaaa gcggaaaatc agtatgcggt cctgaaagag
      481 gcagccacga aacggcagtt atctgagcag gaaaaatccc tgctgaccca tgagaa
//
LOCUS       DAZFAH010000562          534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFAH010000562 DAZFAH010000000
VERSION     DAZFAH010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.565"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>534
                     /locus_tag="ACHJWB_005287"
     CDS             <1..>534
                     /locus_tag="ACHJWB_005287"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4316697.1"
                     /translation="IIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSK
                     SDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIE
                     AMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQG
                     QDFVFEHYSWDGVTQRF"
BASE COUNT          166 a           91 c          126 g          151 t
ORIGIN      
        1 aattattttt tatgctgggc gtatcgtccc tgacaaaggc atattgctgt tgatgcaatc
       61 attcgaaaaa ttggccgctg ctcacaaaaa tttaaaatta gttgtgattg gtgattatac
      121 agaaatgagt aaaagtgaca aaggtgcata tcaacgcaat gtcagggaaa ttgcaaaacg
      181 tttgaaggat cgttgcatta tgttaggaag cataccgcct gaaaagatgc attgttatta
      241 cccactggca gatttagtcg tcattccttc ccagttccag gaaccatttt gcatggtggc
      301 aattgaagct atgggtgctg gtaagcctgt tttagtgagt acgcgtgggg ggatgacgga
      361 atttgtcaaa gaaaatacta cgggattcca tttaaaagaa cctatgactg cagactccat
      421 aagcagcgat attttaaaaa cgttagcgaa tcctgaatta acagcagttg caaagcaagg
      481 tcaggacttt gtgtttgaac actacagttg ggatggcgta actcaaagat tcga
//
LOCUS       DAZFAH010000563          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFAH010000563 DAZFAH010000000
VERSION     DAZFAH010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.566"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..199
                     /locus_tag="ACHJWB_005288"
                     /pseudo
     CDS             17..199
                     /locus_tag="ACHJWB_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          172 a           72 c          151 g          122 t
ORIGIN      
        1 aaagggcggt agtgaaatgc gaaagttcaa aattattatt gaaacgggaa tagctggtgg
       61 agattttgag gatgtattcg aagtggacga tgacgcaaca cctgaggaaa ttcatgacgg
      121 agcataagaa attttcttta actactgcaa ttactcatac cacgaaataa aagacgatga
      181 ggaagaacaa agtggctgat ttttgttcag ctaaatataa catcagtttt gaagagcggg
      241 atgaactatt aatggactat ggtgagttac gcggtggaag tgctgctgat gtcgaatcct
      301 agcgtgatga ctatgaagcg ggaaaaactc cggtcgaagc atattgtgat gagtggggcg
      361 atgaatgagc gagattaatt atcaggaagg gcatgaaacg gcggggcaag caaaaccagt
      421 ggcatggcga tatcgctacg tgaaaaaagg cgttacggac tttcagggga agtagtggtc
      481 tggtgactgg aaatatgtac cgaaaaaaga ggattgt
//
LOCUS       DAZFAH010000564          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFAH010000564 DAZFAH010000000
VERSION     DAZFAH010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.567"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            193..>513
                     /locus_tag="ACHJWB_005289"
     CDS             193..>513
                     /locus_tag="ACHJWB_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO4316698.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTD"
BASE COUNT          119 a          141 c          137 g          116 t
ORIGIN      
        1 gccagccaga cccggcatga ttactgcccc cagtcgtcca tgatccgggg ggtgatgtca
       61 ccgggtctgg tggggcgctg gtaaccgcta ataggggtca ggtcaggcac ttttgccggg
      121 accgtctgta acgtggatgc cggtacctgc tccccgtggt tatctggtta acccatatac
      181 aagggagaca gaatgaccga atccagcgat tacgaatccg tccaggtctt tatcggcgtt
      241 gatgtcggta aagatacgca tcacgctgta gccattaatc gttcaggtaa acgcctgttc
      301 gataaagcat tacccaacga cgaaaacaaa ctcaggtcgc taatatctga cctgaaacaa
      361 catggtcaga tactgctggt tgttgatcag ccagctacca tcggtgcgtt acctgtcgcc
      421 gttgcccgct cagaaggagt ccttgtcgga tacctccctg gactggccat gcgccgcata
      481 gccgacttac acgccggtga agctaaaact gat
//
LOCUS       DAZFAH010000565          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFAH010000565 DAZFAH010000000
VERSION     DAZFAH010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.568"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>507
                     /locus_tag="ACHJWB_005290"
     CDS             <1..>507
                     /locus_tag="ACHJWB_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4316699.1"
                     /translation="AGGWSLYAYPLNPVQHVDPLGLSTMIIGNGPVPDNPFGHAAAAN
                     RYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKT
                     INLGPLPVANNCFSRTNMIFEACGFSNPSMNTNAPISLQVLGELYGYQRYFMPKGPFT
                     GFPFEFQEV"
BASE COUNT          151 a           94 c          121 g          141 t
ORIGIN      
        1 gcggggggat ggagtctgta tgcgtatccg ctgaatccag tgcaacacgt tgatccatta
       61 gggcttagta ctatgataat aggtaacggc cccgttcctg ataatccttt tgggcatgct
      121 gcggcggcaa atcgatatgg attaatgagt tcgggaactg gggatgagat gggagcctct
      181 gtctctgatt attttaaaaa gatgcaaccc cgcagagata cctggatttg gataatagat
      241 acaacagatg aagaagaaca atgtatgatg aataaggcaa tagaccttaa taaaaaacta
      301 aaaactatta acctcgggcc tcttcctgtt gcaaataatt gttttagccg gacgaacatg
      361 atttttgagg cgtgcgggtt tagcaacccc tctatgaata caaatgcgcc tataagccta
      421 caagtgctcg gtgagttata tggatatcaa agatatttta tgcctaaagg tcctttcact
      481 ggattcccat ttgaatttca agaggta
//
LOCUS       DAZFAH010000566          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFAH010000566 DAZFAH010000000
VERSION     DAZFAH010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.569"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..466)
                     /locus_tag="ACHJWB_005291"
     CDS             complement(<1..466)
                     /locus_tag="ACHJWB_005291"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO4316700.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRT"
BASE COUNT          114 a          134 c          102 g          157 t
ORIGIN      
        1 ttgttcgcct taaccatcct gatatttgct ctggagacca tttcatctcc agcttttcca
       61 gaacaagctt tcgcaatggt aaattttgat ccagtaagca cggttttggc cttttcgcca
      121 ttctgttggc tcggttatta gcatcaacag ctttgtaata gcgtctgccc cgattacgct
      181 gaacttcacg tgagatcgtc gaaggactgc gattcagcgc agtagctatc gcacgaatgc
      241 tcattttggc tgacaaacca gctcgtatct cctcgcgctc agacagtgtc aggtgagcta
      301 cagcccgctt acgctcatgg ggttttatgc cgccagtatc ccttaacata gtgaagatcg
      361 ttccgggttt tgaacccagg atattcgcta tttcactgaa gcctgttccg ttcttccata
      421 gttcaaaaac agaggctttt tcctctgctg taaatgttcg tctcattcaa aaaacctccg
      481 caaccccatg ttttcacata actgttg
//
LOCUS       DAZFAH010000567          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFAH010000567 DAZFAH010000000
VERSION     DAZFAH010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.570"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>506)
                     /locus_tag="ACHJWB_005292"
     CDS             complement(<1..>506)
                     /locus_tag="ACHJWB_005292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316701.1"
                     /translation="QLTAVLRAKAKQYKCMFFDTYGMWQDSRGAAGNWLDNPYGDGRG
                     IHPNYVFSAQIHHELLDYIYGPCKVVNGSSNLFSNNGSSLQFVGSSYPPNTFHMGTSW
                     WRTNPDDSSWPLDGVIQVDRFVDGVVLQKLYGYISESSRVCFTRIGRISENTWSQWRG
                     KQYDISGL"
BASE COUNT          160 a          119 c           82 g          145 t
ORIGIN      
        1 caggccagat atgtcatatt gcttaccacg ccactgtgac catgtgttct cacttatcct
       61 tcctatgcga gtaaaacaca ctctgctact ttcagatata tacccatata atttttgcaa
      121 cactactcca tcaacaaatc tatcgacctg aataacacca tcaagaggcc aagaagaatc
      181 atcaggattt gttctccacc atgaagtccc catatgaaac gtatttggtg gatatgaact
      241 accaacaaat tgcaaagatg aaccattgtt agaaaataaa ttagaactgc catttactac
      301 tttgcatggg ccatatatgt aatcaagtaa ctcatggtga atttgggcgc taaaaacata
      361 atttggatga attccgcgac catccccata tggattatca agccagttac ctgctgcgcc
      421 tctggaatcc tgccacatac cgtaagtgtc aaaaaacatg cacttatatt gtttagcttt
      481 agctctaagc acggctgtta actgtt
//
LOCUS       DAZFAH010000568          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFAH010000568 DAZFAH010000000
VERSION     DAZFAH010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.571"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          119 a          110 c          135 g          138 t
ORIGIN      
        1 caattaatca ttaatttatt cacaggtgag gtagagtgcg tgcgccggac acggataaga
       61 atccggcact gacagtttac tgaaaaggat atatccctga aaagtcaggg cataacacga
      121 aagcgcccgg cgaagttagt ctctctgtat aggtcgtcgt taaatttaat tcgatcgtgc
      181 gcttccggtt gtggcaatcc gcgaaatggc gcggcggtaa gtatggcggg gttattcctt
      241 ccccgttgag gacaccgggt tgtcaggttg accatacgct taagtgacaa ccccgctgca
      301 acgccctctg ttatcaattt tctggtgacg tttggcggta tcagttttac tccgtgactg
      361 ctctgccgcc ctttttaaag tgaattttgt gatgcggtga atgcggctga gcgcacgcgg
      421 aacagttaaa accaaaaaca gtgttatggg tggattctct gtatccggcg ttaattgtta
      481 actggttaac gtcacctgga gg
//
LOCUS       DAZFAH010000569          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFAH010000569 DAZFAH010000000
VERSION     DAZFAH010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.572"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..500)
                     /locus_tag="ACHJWB_005293"
     CDS             complement(<1..500)
                     /locus_tag="ACHJWB_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4316702.1"
                     /translation="MSAFHNGRILVEPEPKSMRALPSGVVPAVHQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWLHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     EQTSDSLDQLAQQNLAAWMIDIIRHAMNGAQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLR"
BASE COUNT          114 a          149 c          130 g          107 t
ORIGIN      
        1 cgcaacccca gcgaacgacg taataccgct tccggtagcg cgtccgccac ctgattgcgg
       61 accgcccacc aggataattc agccagagat aattcacgct cctgtgcgcc attcattgcg
      121 tgacggatga tgtcaatcat ccaggcggcc agattctgtt gagcaagttg atccagtgaa
      181 tcagatgtct gctcccgcag ctggttgtcg cagtgccagc acaacaccat cgcgccggta
      241 ccgtaacggt gaatgacggt ttcgctgtga tgataatcac cgtgtagcca ctggcaggat
      301 ttcacgtgac gtaataacca gtcagacagt gcaccagcgc cacccgcagc acggatcacc
      361 cgctcatcgc tgaaaaatgg cagtagtgat ttatcttccg ccagcggctg gtgaacggca
      421 ggtacaaccc cggacggcag agctcgcatg ctttttggtt ccggctccac cagtattctg
      481 ccgttatgga atgctgacat
//
LOCUS       DAZFAH010000570          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFAH010000570 DAZFAH010000000
VERSION     DAZFAH010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.573"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            149..498
                     /locus_tag="ACHJWB_005294"
                     /pseudo
     CDS             149..498
                     /locus_tag="ACHJWB_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a          126 c          145 g          110 t
ORIGIN      
        1 agttttgtcg agttctttga caatagcgat aaagatttat atgcgtttaa atcctaaaat
       61 gaaaatattg gattaatctg tgtcgttcag actctatatt gagttattgc gaaccgggtg
      121 ttgcaggtgc acccttccgt gaccctgcaa ccgccacggc aacctgctgg cgtacacgga
      181 cggtaacggt gttgtctgga caatggagta cggcccgttc gatttgccgg tggcgcgaac
      241 ggacggtgaa ggccaccgct ggcagtaccg ctacgataaa gacacgctgc aactgacaga
      301 agtcattaac ccgcagggcg aaacatacct ttatgttctg gataactgcg gccgggtgac
      361 ggaagagcgt gactggggcg gcgtggtctg gcgttaccgc tatgacgccg atggcctctg
      421 taccgccagg gtcaacggac tggaggaaac catcctctac agccgggacg ccgcaggccg
      481 cctggcagaa gtcatcac
//
LOCUS       DAZFAH010000571          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFAH010000571 DAZFAH010000000
VERSION     DAZFAH010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.574"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          144 a           89 c          116 g          149 t
ORIGIN      
        1 caagctaact cagtgagcgt ggtgaaaatc agtgtttacc cgccatcagg ctgagcataa
       61 ttctcatcat gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt ctgaagtcga
      121 tctggagagg cttgttgatg ttggtgtttt caggatgatg tttcacttag tttgtttgcc
      181 gtatcgcccg gcgaatggct gtgattgagg aaggtttaag tcgtagtgac caaagctata
      241 tttaccaacg aatgtagatg aaaaaatcat ctcctgcgtt cccccatatc tccaggataa
      301 aaaggaatgt aacaatctca tggcgtaagc tgacgaatca gccaggaata atcgctaggg
      361 acctaagaat tagcatgata atagccacta agaaattact gcgctccatg aaatagccat
      421 tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt gaacgaatat
      481 taaattttgc tgaatttt
//
LOCUS       DAZFAH010000572          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFAH010000572 DAZFAH010000000
VERSION     DAZFAH010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.575"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..191)
                     /locus_tag="ACHJWB_005295"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="ACHJWB_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            341..>496
                     /locus_tag="ACHJWB_005296"
     CDS             341..>496
                     /locus_tag="ACHJWB_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4316703.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEH"
BASE COUNT          147 a          120 c           90 g          139 t
ORIGIN      
        1 gagatagcaa taaccgttac tgtaacatga gttacaatca gcttcatacc cagagctact
       61 gatgtatcaa cacgattcaa caatacccga tatttattcc atgcctccag caacgatctt
      121 tcttcctccg ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg
      181 aactcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
      241 gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt
      301 catcgccata attgatttaa ttcacaaata aaactataac atggtgaaat taatgaaaaa
      361 aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg
      421 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac acctccttcc
      481 acactttctg gaacat
//
LOCUS       DAZFAH010000573          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFAH010000573 DAZFAH010000000
VERSION     DAZFAH010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.576"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>484
                     /locus_tag="ACHJWB_005297"
     CDS             33..>484
                     /locus_tag="ACHJWB_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4316704.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNE"
BASE COUNT          142 a          101 c          124 g          117 t
ORIGIN      
        1 agacaaggtt catcactaaa aggaaataac cgatgaatat gatggccgtg ccgtttcacg
       61 gcaactctct ttatgtagtt aaccataatg gcgaaccata cgttcccatg aaacctgtcg
      121 ttgcggggat ggggctggcc tggcaatcac agttggctaa gttaagacag cgttttgcgt
      181 caactataac ggaaatcgtt atggttgctg aggatgggaa acaacgcaat atggtgtcca
      241 tgccacttcg aaaacttgcc ggctggctac aaaccattaa tcccaacaaa gtaaaacccg
      301 aaatccgcga taaggtcatc cggtatcagg aagagtgcga cgatgttctt tacgagtact
      361 ggacgaaggg ttttgttgtt aatccccgta aaatgagcgt gatggaagaa ctcaaccagg
      421 cttgtgctga catgaaacgg gataaaaaca ttgccagtgt gtttgctacc gggctgaatg
      481 agtg
//
LOCUS       DAZFAH010000574          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFAH010000574 DAZFAH010000000
VERSION     DAZFAH010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.577"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>479
                     /locus_tag="ACHJWB_005298"
     CDS             <1..>479
                     /locus_tag="ACHJWB_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4316705.1"
                     /translation="SQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWAS
                     SVKEGASNDFKENVDRALVRLFKCVELHSNELNLSYLFLGSVPPLPEWIEMLSLIHNE
                     LDSIHVPESCKELEVDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKIISICH"
BASE COUNT          150 a           91 c           94 g          144 t
ORIGIN      
        1 agtcaggggg aggggacttt acccattcga cataaattag agtttatatc gaccaatatt
       61 gcagagttgc tggacaagtt aaccaaaatt acagatgcca gactttgcaa aggtttcagt
      121 gactgggcta gttcagtcaa agaaggcgca tccaatgact tcaaagaaaa tgtggataga
      181 gcattggtga gattgtttaa atgtgttgag ctccacagta atgaacttaa cttatcatat
      241 cttttcttgg gttctgtgcc gcctcttccc gagtggattg aaatgcttag ccttattcat
      301 aatgaacttg attcaataca tgtgcccgaa tcatgcaaag aattagaagt cgatttcaat
      361 aaccttacag aatttccaca agtacctgat ggaattaccc tgatctccgt aaataataac
      421 ctgatatcgc atattgactc atttccgcca aaggctaaga taatttctat ttgtcataa
//
LOCUS       DAZFAH010000575          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFAH010000575 DAZFAH010000000
VERSION     DAZFAH010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.578"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..>478
                     /locus_tag="ACHJWB_005299"
     CDS             15..>478
                     /locus_tag="ACHJWB_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4316706.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GTGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNICPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRS"
BASE COUNT          123 a          117 c          135 g          103 t
ORIGIN      
        1 cctgataaat atccatgaac accaaaatca aatacggcct gtcggctgcc gttctggcgc
       61 tgattgccgc aggtgcgcct gcacctgaaa tcctcgacca gtttctggat gaaaaggaag
      121 gtaaccacac cacagcatac cgtgatggta cgggtatctg gaccatctgc cgcggtgcca
      181 tcctggtgga tggtaaacct gtcgtcccgg gcatgaagtt gtcgaaggaa aaatgcgacc
      241 aggttaacgc cattgagcgt gataaggcgc tggcatgggt ggagaaaaac atcaaagtgc
      301 cgctgaccga accccagaaa gcggggatcg cgtcattctg tccgtacaac atttgtcccg
      361 gtaagtgttt cccgtcgacg ttttacagac gaattaatgc aggtgatcga aaaggtgcct
      421 gcgaagcgat tcgctggtgg attaaggacg gtggcagaga ctgccgtatt cgttcaaa
//
LOCUS       DAZFAH010000576          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFAH010000576 DAZFAH010000000
VERSION     DAZFAH010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.579"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..159)
                     /gene="yciX"
                     /locus_tag="ACHJWB_005300"
     CDS             complement(<1..159)
                     /gene="yciX"
                     /locus_tag="ACHJWB_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4316707.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQP"
     gene            complement(162..302)
                     /gene="ymiA"
                     /locus_tag="ACHJWB_005301"
     CDS             complement(162..302)
                     /gene="ymiA"
                     /locus_tag="ACHJWB_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4316708.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          140 a          112 c           98 g          128 t
ORIGIN      
        1 cggctgatca tcttcagcaa acagttctat aaaggcttgc tgctgcgatg ttagttgcca
       61 gtcgtctgga ataacgacgg aacattcccg tttcgtttct gtttcattgt cactatgctg
      121 gcataatggt gacgactcca gttgctcctg accgaccata gttatcccca cactttccaa
      181 atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa gaaaaaccgc cagccaggct
      241 ttacgtttta attcaggatc gcggcggggt tcctgatttc cagaaggcat tgctaacctc
      301 atataatcgc catcacttat cagcaagacg ttgaacgatt ggtgtaatta atcatacgat
      361 gagacaaatc ctaaagaaac tttagtcaaa aatccagcga atttacgaat cgtctccagt
      421 gaataattca atcttttgac ctgaaataga tatttgaaaa ctggagtttg cgaggcgg
//
LOCUS       DAZFAH010000577          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFAH010000577 DAZFAH010000000
VERSION     DAZFAH010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.580"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            274..399
                     /locus_tag="ACHJWB_005302"
                     /pseudo
     CDS             274..399
                     /locus_tag="ACHJWB_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
BASE COUNT          157 a          105 c           93 g          122 t
ORIGIN      
        1 ataagaagcg agccgttgcg gaggcttgct ctctccttta acctcatgtg caatatccgt
       61 aacgcgctca cataaatcat catctgataa tataacaaca cccattattt tattctgacc
      121 taaaaacttc ttatgaaata aacgtttacc cgacgtatct gatcccgctg tttttacctg
      181 tatcctttaa catacttgag aagcccgctg aaaaatatac cgtaaaggcg gttctctgtc
      241 ttccataaac ctgtcggata agaagggcat taaatgaaaa ccaaaaaagt tatgatgatt
      301 gcactggtca gctctgcgct ggctctttca ggatgtggag ccatgggcac agcgatcaaa
      361 aaacgaaatc tggaagtaaa aacccagatg agtgaaacct gaggtagcct gagtttaacg
      421 gacactcctt cctgaaatag aatggcatca gaaggaacta ataatgagca gaaaaac
//
LOCUS       DAZFAH010000578          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFAH010000578 DAZFAH010000000
VERSION     DAZFAH010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.581"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..471
                     /locus_tag="ACHJWB_005303"
                     /pseudo
     CDS             1..471
                     /locus_tag="ACHJWB_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     misc_feature    275..391
                     /locus_tag="ACHJWB_005303"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          109 a          120 c          140 g          102 t
ORIGIN      
        1 aaaatactcg tttttccccc gaagtccgtc agcgggcgat tcgtatggtt ctggaaagtc
       61 aggatgaata tgactcacag tgggcggcaa tttgttccat tgccccaaag attggctgta
      121 cgccggagac tctgcgtgtc tgggttcgcc agcatgagcg ggataccggg ggcggtgatg
      181 gtgggctcac cagcgctgaa cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac
      241 tgcgccgcag taacgatatc cttcgccagg cttccgctta ttttgcgaag gcggagttcg
      301 accgcctctg gaaaaaatga tgccactgct ggataagctg cgtgagcagt acggggtcgg
      361 accggtatgc agcgaactgc atattgcccc gtcaacgtat taccattgtc agcaacagcg
      421 acatcatccg gataaacgca gtgcccgtgc gcagcacgat gactggctga a
//
LOCUS       DAZFAH010000579          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFAH010000579 DAZFAH010000000
VERSION     DAZFAH010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.582"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..>461
                     /locus_tag="ACHJWB_005304"
     CDS             119..>461
                     /locus_tag="ACHJWB_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316709.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKH"
BASE COUNT          147 a           78 c           92 g          144 t
ORIGIN      
        1 tatagcaaat acagattaat cgcaattttt cccagcaaaa ttacaaaatc atggtgtatt
       61 gacatcatca atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat
      121 ggggctttgt agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat
      181 aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac tcgtgttttt gtatgtggcc
      241 cgaagaaagt tcatatttta atcgtggtgt ggtagaaggt atcttaacaa aaaaccacaa
      301 tgcgagatta agcggatata tttttgtcga tttttcagta agttttttac gtctattcct
      361 tgaaaaagac tggattgact atcttgcaag tactgacatg gggattgttt tagtcagcga
      421 ccgcaatatg caatcgttag ctaattactg gcgaaaacac a
//
LOCUS       DAZFAH010000580          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFAH010000580 DAZFAH010000000
VERSION     DAZFAH010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.583"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..365)
                     /locus_tag="ACHJWB_005305"
     CDS             complement(<1..365)
                     /locus_tag="ACHJWB_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4316710.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHA"
BASE COUNT          106 a          126 c          120 g          108 t
ORIGIN      
        1 gcatgctctt cctgtttgag gttatcggtg atgatacgca gacgacggta ggccgggtcg
       61 ttcagttgag ccggatcttc accgggaaga cgctcaaccg cctgctggta attaaattcg
      121 tgtttcggct tgacgtagcc cggacgtaac acgcgggttt caccaccgcg atgacgcagc
      181 acttttcctt caacaaccgg ggagacatag gccgccaccg gcgtttttcc ggtaattttg
      241 tccagcatca cctcttcggt atggaaattc accgtacggc ggaaaaacag ctccagaaac
      301 agcgcacgaa atttcacttt ttgttcggta taaccgagta actggcgggt cgtaaacaat
      361 cccataaatc agttcctttc attcagaaat cagtcaggcc accgcggtgg cctgataacg
      421 tgttacggca gcgccgcgtg actcagggca ctgccggcaa
//
LOCUS       DAZFAH010000581          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFAH010000581 DAZFAH010000000
VERSION     DAZFAH010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.584"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>460
                     /locus_tag="ACHJWB_005306"
     CDS             <1..>460
                     /locus_tag="ACHJWB_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316711.1"
                     /translation="LLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCH
                     GGSAADVPWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATN
                     APAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAIL"
BASE COUNT          109 a          118 c          131 g          102 t
ORIGIN      
        1 tgttgctggc ggtgtgctgg atgcagggag agtttgacat gagcgccgcc acccacgcac
       61 agcaacctgc gctgtttaca gccatgctga cacagtttcg tgctgacctc tccgtgttta
      121 acgcgcagtg ccatggtggc agtgctgcag atgtgccgtg gatttgtggt gacacgacgt
      181 attactggaa aaatacatac gctacccagt acgacaccgt gtacggcggg tataaaaaca
      241 gggagagtga gggcgtttat tttgtgccct tcatgacaga cggtaacggc gtcaataccg
      301 ccactaacgc gccggcagaa gatccggata ttccggcatc aggatattac ggtgcggcat
      361 cgagaacgaa tggaaaccag gtatcatcaa accgcccgac acatttcagt tcatgggcgc
      421 gcaggagcat tattccggat cgtctggcaa ccgctattct
//
LOCUS       DAZFAH010000582          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFAH010000582 DAZFAH010000000
VERSION     DAZFAH010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.585"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>457
                     /locus_tag="ACHJWB_005307"
     CDS             <1..>457
                     /locus_tag="ACHJWB_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316712.1"
                     /translation="LADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFRNRLN
                     LSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPENHKPD
                     PEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEK"
BASE COUNT          127 a          108 c          110 g          112 t
ORIGIN      
        1 actggcagac ggaaactggt tactgaacaa tgcctggtat gaaagaaaca tggcagggtt
       61 taacgaacag ttgaaagaga acctgtcatt cacacctgat gaactgaaaa cgctcttccg
      121 gaaccgcctg aatttatcac cggaagcgaa tgacgatttt ctcgatcgtt gcctggacgg
      181 cggtgactgg tatccctttt cagaaggtcg ccgttttgta tcattccatc acgtggatga
      241 gcttcgtatc tgtgcctcct gcgggctgac agaagtacat catgccccgg aaaatcataa
      301 gccggatccg gaatggtact gctcctctct ttgtcgcgaa acagaaacac tgtgtcagga
      361 aatttatgaa cgcccttaca acagctttat ttccgatgca acggcgaatg gtctgattct
      421 catgaaactg ccggaaacct ggagtacaaa tgagaaa
//
LOCUS       DAZFAH010000583          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFAH010000583 DAZFAH010000000
VERSION     DAZFAH010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.586"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHJWB_005308"
     CDS             <1..>453
                     /locus_tag="ACHJWB_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFO4316713.1"
                     /translation="KTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVN
                     GTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVN
                     QALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP"
BASE COUNT          137 a          113 c          100 g          103 t
ORIGIN      
        1 taaaacgctg tactttcgta gccgtgaagc gctacaccac ctgaatatac agcatctgcg
       61 acggtcgcat agccttcgcc atggcaggcg tcatacccgc aaaggcgaaa gaggtacgat
      121 taacatagtg aacggaacac caattcacga acgttcccga aatatcgata acagacgctc
      181 tctggggcat tgggagggcg atttagtctc aggtacaaaa aactctcata tagccacact
      241 tgtagaccga aaatcacgtt atacgatcat ccttagactc aggggcaaag attctgtctc
      301 agtaaatcag gctcttaccg acaaattcct gagtttaccg tcagaactca gaaaatcact
      361 gacatgggac agaggaatgg aactggccag acatctagaa tttactgtca gcaccggcgt
      421 taaagtttac ttctgcgatc ctcagagtcc ttg
//
LOCUS       DAZFAH010000584          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFAH010000584 DAZFAH010000000
VERSION     DAZFAH010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.587"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>449
                     /locus_tag="ACHJWB_005309"
     CDS             <1..>449
                     /locus_tag="ACHJWB_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316714.1"
                     /translation="WGRGSMIALMVAEFIAINPDAELYAIAQGNGTGQATAAAMNLTG
                     TVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPFTASSGAGSGDNG
                     AGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQKA"
BASE COUNT          102 a          122 c          139 g           86 t
ORIGIN      
        1 tctgggggcg cggctccatg attgccctga tggtggcgga atttatcgcc atcaatcctg
       61 atgctgaact gtatgccatt gctcagggga acggcaccgg acaggcaacg gctgcggcca
      121 tgaatctgac cggaacggtc acccgtgacg ggattgttta tgcctgcgtg ggggggcggc
      181 gttatacact cccggcacca aaaggtaaga aaggcaaaga actgaccgac gagctggccg
      241 cgctgatcaa tgccgatcct gacgcgccct tcacggcctc ctctggtgca ggaagtggtg
      301 ataatggcgc aggtctgaaa gggtctctgg ggatcaccgc ccgctttacc ggtgaatgct
      361 ccgttcatga tgtgcgcctg aactatcacg acggggaagc aaccccggaa ggaatacagg
      421 tcgctatagc ctatccgaaa caaaaagcg
//
LOCUS       DAZFAH010000585          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFAH010000585 DAZFAH010000000
VERSION     DAZFAH010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.588"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          128 a           87 c          102 g          129 t
ORIGIN      
        1 aaaagtagct cacgtgcaga tttaaacaca tcggaaacaa aaatatcttt aagtaatgac
       61 gctctgttat taagagatat attcgagtta tctaagcaaa ccagtgggga tgttctaatc
      121 tataatactc tttggcaata tcattaatcc attatatatg ccctctgaat ttttcagctc
      181 cgtgaagttc gctctggaca ggatagggtg gaatcagcct tgtctgtgag ctttgttgcc
      241 tggatttctg cgccggtcag tgccataggt tagccctctg tatacgttaa agagggcaca
      301 aacactgtct gactggggga tacccgcgat gacattcggg caatgacgtt gacactgaag
      361 aataaagctc tgaaatgaaa aacggacctc catggaggtc cgttgatatg aatttggtgc
      421 cggactcgga atcgaaaatt tctttt
//
LOCUS       DAZFAH010000586          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFAH010000586 DAZFAH010000000
VERSION     DAZFAH010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.589"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>446)
                     /locus_tag="ACHJWB_005310"
     CDS             complement(<1..>446)
                     /locus_tag="ACHJWB_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316715.1"
                     /translation="WGRGSMIALMVAEFIAINPDAELYAIAQGSGTGQAQACLMNIIG
                     TASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPFSAYAGQVADNAS
                     GLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA"
BASE COUNT          114 a          126 c          103 g          103 t
ORIGIN      
        1 ggcttttgtt ttgggatacc caagttcaat ggtcagtcca cccggtgtgg tttctccgtc
       61 atgataatta acacggatat catgtacgga acattcaccg gtaaaacggg cattaattcc
      121 catacttccc ttgagtccgc tggcgttatc tgctacctga cctgcataag cactgaaggg
      181 cgcatcagca tcggcattaa taacagagac cagagcgtca ataagctcct tacccttctg
      241 ccctttcgtg acagccagca gataacgccg accgccgata taaatgtgca gaacaccatc
      301 ctgagaggcg gtgccaatta tattcatgag acatgcctga gcctgaccgg tgccgctccc
      361 ctgagcaatg gcatacagtt cagcatcagg attgatggcg ataaattccg ccaccatcag
      421 ggcaatcatg gagccgcgcc cccaga
//
LOCUS       DAZFAH010000587          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFAH010000587 DAZFAH010000000
VERSION     DAZFAH010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.590"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHJWB_005311"
     CDS             complement(<1..209)
                     /locus_tag="ACHJWB_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4316716.1"
                     /translation="MWKPLRDENRLSILKIYGYTCDRLVREEERQNITSISRSQAWKL
                     EREGKFPPRKTIGKKSCGWLLSDLL"
BASE COUNT          146 a           98 c           65 g          136 t
ORIGIN      
        1 cacaatagat cacttaataa ccatccacag gatttctttc caatagtttt acgtggcggg
       61 aattttcctt cacgttccag tttccaggct tgtgaacgag aaatagacgt aatgttctga
      121 cgttcctctt ctcgtaccag tctgtcacag gtgtatccat agatcttcaa aattgataaa
      181 cggttctcat ctcttaacgg cttccacata ttgctctcct tatacattca tgattttcag
      241 attgtatatg catgtgagct gctcaaatca ctacatctat ctgtagtggt atgctgctga
      301 acagcaccag caactcacga gcaaaacaat acatttgcac aaccaagaaa tcaaattgcg
      361 agaattgatt taattaactg acttctgata acacaataac aacaaagaaa gtcacaatat
      421 aaaaataatt caaacaatac ttttt
//
LOCUS       DAZFAH010000588          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFAH010000588 DAZFAH010000000
VERSION     DAZFAH010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.591"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>441
                     /locus_tag="ACHJWB_005312"
     CDS             <1..>441
                     /locus_tag="ACHJWB_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4316717.1"
                     /translation="VRLPEQFSIIGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPD
                     GRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPT
                     HRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIRTGKRWA"
BASE COUNT          108 a          113 c          130 g           90 t
ORIGIN      
        1 atgtcagact ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg
       61 tcacactctg ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc
      121 ctgaaaaacc agacggcaga cgggctgtat tacgcagtca ggtacttgag ctacatggca
      181 tcagccacgg ttcggccgga gcaagaagca tcgccacaat ggcaacccgg agaggctacc
      241 agatgggacg ctggcttgct ggcaggctca tgaaagagct ggggctggtc agctgtcagc
      301 agccgactca ccggtataaa cgtggtggtc atgaacatgt tgctatccct aactaccttg
      361 aaaggcagtt cgccgtgacc gagccaaatc aggtgtggtg cggtgatgtg acctatatcc
      421 ggacgggtaa gcgctgggcg t
//
LOCUS       DAZFAH010000589          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFAH010000589 DAZFAH010000000
VERSION     DAZFAH010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.592"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>433
                     /locus_tag="ACHJWB_005313"
     CDS             <1..>433
                     /locus_tag="ACHJWB_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HFO4316718.1"
                     /translation="LVDPVTWESIGYTGSDSYIKSPISSPTLHDISIRAKYRALEWRS
                     FYGHNARLWHETVIKYSGSEPRYHPQMLLSPNEGRCIGLSELYILADTKEKYNTLQEN
                     LDLISSLYQQDISDNAHLSESDHRLLKNTVSQIEYYQQHGNN"
BASE COUNT          151 a           75 c           82 g          125 t
ORIGIN      
        1 ttagttgatc ccgtaacatg ggaaagtata ggctatacag gaagcgatag ttatattaaa
       61 tcgccaatat catctccgac attacatgat atttctattc gggcaaaata tcgagctctg
      121 gaatggagaa gcttttatgg gcacaatgcc agattatggc atgaaaccgt gataaaatac
      181 tccggtagtg agccaagata tcatccccag atgttgcttt cacccaatga agggcgttgt
      241 atcggtttaa gtgagttata tatccttgct gatactaaag aaaaatataa tacgttacaa
      301 gagaaccttg atttaatcag ctcattatat caacaagata tatctgacaa tgcacatctt
      361 tcagaaagtg atcatcgatt gttgaaaaac actgtatcac aaattgaata ttatcagcag
      421 catgggaata ata
//
LOCUS       DAZFAH010000590          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFAH010000590 DAZFAH010000000
VERSION     DAZFAH010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.593"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>427
                     /locus_tag="ACHJWB_005314"
     CDS             <1..>427
                     /locus_tag="ACHJWB_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4316719.1"
                     /translation="PLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSD
                     RDYTWNDNGELIRISSPRQTRSYSYSTTGRLTSVHTTAANLDIRIPYATDPAGNRLPD
                     PELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLI"
BASE COUNT           97 a          160 c          108 g           62 t
ORIGIN      
        1 ccgctggtgg agtacacccg cgaccgcctg caccgggaaa cgctgcgcag cttcggccgt
       61 tatgaactca ccaccgctta tacccctgcc gggcagttac agagccagca cctgaacagc
      121 ctgctgtctg accgcgatta cacctggaac gacaacggcg aactcatccg catcagcagc
      181 ccgcgccaga cccggagtta cagctacagc accaccggca ggctgaccag cgttcacacc
      241 accgcagcga atctggatat ccgcatcccg tatgccacag acccggcagg taaccgcctg
      301 cccgacccgg agctgcaccc ggacagcacc ctcagcatgt ggccggataa ccgtatcgcc
      361 cgtgacgcgc actatcttta ccggtatgac cgtcacggca ggctgacaga gaaaaccgac
      421 ctcatcc
//
LOCUS       DAZFAH010000591          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFAH010000591 DAZFAH010000000
VERSION     DAZFAH010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.594"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>425
                     /gene="espX1"
                     /locus_tag="ACHJWB_005315"
     CDS             <1..>425
                     /gene="espX1"
                     /locus_tag="ACHJWB_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4316720.1"
                     /translation="ITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIK
                     KSNIFGTSFTNTMIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFE
                     ILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMR"
BASE COUNT          160 a           73 c           67 g          125 t
ORIGIN      
        1 atataacaat agaaaagagc aacctttcat atgggtatta ttttggttgt gtattatcca
       61 atatatcatg ttttgaaagc gacttatcaa acaccatctt ttcaaatgga gaaataaaca
      121 acctcttcat taaaaaaagc aacatatttg gaacatcgtt cactaatacc atgataaaaa
      181 atttgctctg tgaagatatt atgcctggtc gatggaccac tcaacttgtc aataagcact
      241 taggttacag atacacagga gtattcaaaa ccctggcaag tattgatgac aaaccgtccc
      301 gtttcgaaat tttgatacca ttggtacaaa ctttagttcg cgacaatgta aagctaaata
      361 atgatgtgta taaagaactt aataagttta tgcacgatta cgacaaaaca tcatcagaaa
      421 tgaga
//
LOCUS       DAZFAH010000592          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFAH010000592 DAZFAH010000000
VERSION     DAZFAH010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.595"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>418
                     /locus_tag="ACHJWB_005316"
     CDS             <1..>418
                     /locus_tag="ACHJWB_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO4316721.1"
                     /translation="RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAACDRVNRQF
                     VAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSSMETTFVLDALEQAL
                     WARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTG"
BASE COUNT           88 a          105 c          128 g           97 t
ORIGIN      
        1 tcagagtggc cagatgcact gtggcacgtc tcatggcagt tatgggactt gccggtgttc
       61 tccggggtaa aaaggtccgc actaccgtca gccggaaagc cgttgccgca tgcgaccgcg
      121 taaaccgtca gttcgtggca gaacgtccag accagttgtg ggtggctgat tttacctggg
      181 taagcacatg gcagggcttc gtttatgtgg cgttcatcat tgatgtgttc gccggataca
      241 tcgtgggatg gcaggtctca tcatccatgg aaacaacatt cgtgctggat gcactggagc
      301 aggcgttgtg ggcccgtcgg ccgtccggca caatccatca cagtgataaa ggttctcagt
      361 atgtatcgct ggcctacacg cagcggctta aggaagccgg attactggca tcaacagg
//
LOCUS       DAZFAH010000593          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFAH010000593 DAZFAH010000000
VERSION     DAZFAH010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.596"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>417
                     /locus_tag="ACHJWB_005317"
     CDS             <1..>417
                     /locus_tag="ACHJWB_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4316722.1"
                     /translation="AAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPEMTVEK
                     ARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGSGK
                     AEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDA"
BASE COUNT           89 a          120 c          146 g           62 t
ORIGIN      
        1 cgcggcggca ccgcagcagc cggtggcatc cacgcagagt gaagccgatc gcattatggc
       61 gtgtgaagaa gcgaacggtc gtgaacaact ggcggcaacg ctggcggcga tgccggagat
      121 gacggtggaa aaagcccgcc cgatcctggc tgcttcaccg caggcggatg ccggaccctc
      181 actccgtgat cagatcatgg cactggatga ggcaaaaggg gctgaggcgc aggctgaaca
      241 gctggctgcc tgcccgggaa tgactgtgga gagcgcccgg gctgtgctgg ctgcgggatc
      301 aggtaaggca gaaccggtct ctgcatccac aaccgccctg tttgaacgca tcatggcgaa
      361 ccattcaccg gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgc
//
LOCUS       DAZFAH010000594          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFAH010000594 DAZFAH010000000
VERSION     DAZFAH010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.597"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..388
                     /locus_tag="ACHJWB_005318"
     CDS             <1..388
                     /locus_tag="ACHJWB_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316723.1"
                     /translation="KRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKNA
                     ILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDGK
                     IHKNYNRWVRNLDKDIQVNLSTGATL"
BASE COUNT          130 a           85 c           95 g          107 t
ORIGIN      
        1 gaaacgttat ataaaatggt ttgccatcac aatttttatc agtatgttga gtgcctgtgc
       61 ccgtacggcc ccagtgcaac agataagcac cactgtcagt gtgggtcata ctcaggagca
      121 ggttaaaaat gccattttga aagcaggtgc gcagcgcaag tggattatga cgcaagtgtc
      181 ccctggagtt attaaagctc gctatcaaac acgaaatcac gttgcagagg ttcgtattac
      241 atatacagct acctactata acatcaaata tgacagtagc ctgaatctgc aggcttctga
      301 tggaaaaatt cataaaaact ataaccgctg ggtgcgtaac ctagataaag atatacaggt
      361 taacttatct acaggagcaa cgttatagtg atgctgatat cgggctggaa tggcccg
//
LOCUS       DAZFAH010000595          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFAH010000595 DAZFAH010000000
VERSION     DAZFAH010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.598"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>414
                     /locus_tag="ACHJWB_005319"
     CDS             <1..>414
                     /locus_tag="ACHJWB_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4316724.1"
                     /translation="ECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGK
                     ILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDK
                     SLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYK"
BASE COUNT          147 a           76 c           76 g          115 t
ORIGIN      
        1 agaatgttat tgccttctag cggaatgcca tatgtctttg gcacttcatg gaaaaagtga
       61 acttgaactt gctgctcaaa aagcattaga gcttttagat tatgtatcag acataaccac
      121 tgtcgatgga aaaattttag ctattatggg actgataact ggtctgtctg gacaagcaaa
      181 agtatctcat atcttatttg aacaggctaa gatacactca actgatatag cctctctcta
      241 ctactatagg gcactagtcc actttcataa tgaaaaaatt gaagaggcaa ggatttgtat
      301 agacaaatca ctacaactcg aacccagaag acgaaaagca gttgtgataa aagaatgtgt
      361 ggatatgtat gtgcctaacc cgctcaaaaa caacattaaa ctctactata aaga
//
LOCUS       DAZFAH010000596          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFAH010000596 DAZFAH010000000
VERSION     DAZFAH010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.599"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..235
                     /locus_tag="ACHJWB_005320"
     CDS             <1..235
                     /locus_tag="ACHJWB_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO4316725.1"
                     /translation="VTTSDVVMCQMRHSDDQRVYRVYGSWMAENVQDQVSILNQKMSE
                     FAPSMPHAVRSDVINNRLKNLHLHAHHMTMYIS"
BASE COUNT          129 a           96 c           88 g           98 t
ORIGIN      
        1 cgtaacaaca tccgacgtgg tgatgtgtca aatgagacac tctgatgatc agagggttta
       61 tcgcgtatac ggatcgtgga tggcagaaaa cgttcaggat caggtatcca ttttaaacca
      121 gaaaatgagt gagtttgccc catccatgcc ccacgcggtg agatcggatg taatcaacaa
      181 caggttaaaa aacctacacc tgcatgctca tcatatgacc atgtatattt cataaatgac
      241 aaagatcgcc caagagcaca ccagataaca ggcagttaga gttgactgaa acatcaagca
      301 tcgtagttct ccgtagatct aactgtgtga tttgccccat atatgcccca ccactcagca
      361 tagaagagca caaagccttg caatccagtg caaagctttg tgcgtcttat g
//
LOCUS       DAZFAH010000597          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFAH010000597 DAZFAH010000000
VERSION     DAZFAH010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.600"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..152)
                     /gene="gpU"
                     /locus_tag="ACHJWB_005321"
     CDS             complement(<1..152)
                     /gene="gpU"
                     /locus_tag="ACHJWB_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4316726.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAA"
     gene            complement(165..>407)
                     /locus_tag="ACHJWB_005322"
     CDS             complement(165..>407)
                     /locus_tag="ACHJWB_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4316727.1"
                     /translation="LANGRWHVMQRMPWASSSTGADSKGRPKRHRLPIEVVKITTAGP
                     LAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
BASE COUNT           85 a          127 c           93 g          102 t
ORIGIN      
        1 tcagcagcag acgcatctgt caggtaaaca gcaacagcag gcagttcctg ttcatcaata
       61 aaaaccgggc gaccgtcaaa ccagctcacc ctctcagaaa tattttcttt cagggcagac
      121 agaactgccg cccgtatttc acggtgtttc atacaccctc ccagtcattt tcttttcagc
      181 accaggcgta actgatgcgt catggctttc atcatctgcg ccggtaattt ttcccggtac
      241 atccggtccc gttcacgttc aaaggtttct gccagcggtc cggcagtcgt aatcttcacc
      301 acttcgatcg gcagacggtg gcgtttcggc ctccctttgc tgtccgcgcc ggtggatgat
      361 gatgcccacg gcatacgctg catcacatgc cagcgtccgt tagccag
//
LOCUS       DAZFAH010000598          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFAH010000598 DAZFAH010000000
VERSION     DAZFAH010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.601"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(107..274)
                     /locus_tag="ACHJWB_005323"
     CDS             complement(107..274)
                     /locus_tag="ACHJWB_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4316728.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(271..>399)
                     /locus_tag="ACHJWB_005324"
     CDS             complement(271..>399)
                     /locus_tag="ACHJWB_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO4316729.1"
                     /translation="EAEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          105 a          138 c           76 g           80 t
ORIGIN      
        1 cagcacctga atccgcgatt atcccatata cctggttgct gatcgcccct ccgcacaggg
       61 ggattcacca tgcagaagtg tttttaataa acagcaaaca aaaaaatcaa gcattatgca
      121 ggctgtttct ctttatcacc ggccacagca ataccacaat gccgcagacc agcaccccat
      181 ccgccagcac cgacatgatt ctgctggtga aatccaccat caccaccaga aacagcagga
      241 gtgcagccac agccaggcgc agttttaccg tcacaggtga ttctccagac gaagacccag
      301 aacaccggca atctcttcca gcaccttgcg ctcttccggc tcaatttcgc cgtctgcctc
      361 cgcaatggcc accgccacat ccagcacatc ttccgcttc
//
LOCUS       DAZFAH010000599          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFAH010000599 DAZFAH010000000
VERSION     DAZFAH010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.602"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..232
                     /locus_tag="ACHJWB_005325"
     tRNA            157..232
                     /locus_tag="ACHJWB_005325"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Glu,seq:ttc)
BASE COUNT          112 a           89 c           91 g          103 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaagtaaat agcaaggcgt ttacgcgttg
       61 ggagtgaggc tgaagagaat aaggccgttc gctttctatt aatgaaagct caccctacac
      121 gaaaatatca cgcaacgcgt gataagcaat tttcgtgtcc ccttcgtcta gaggcccagg
      181 acaccgccct ttcacggcgg taacaggggt tcgaatcccc taggggacgc cacttgctgg
      241 tttgtgagtg aaagtcgccg accccaatat ctcaaaacag actgttaagt cttgtttgag
      301 atatttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacgaaagtt
      361 gttcgtgagt ctctcaaatt ttcgcaactc tgaag
//
LOCUS       DAZFAH010000600          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFAH010000600 DAZFAH010000000
VERSION     DAZFAH010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.603"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>395
                     /locus_tag="ACHJWB_005326"
     CDS             <1..>395
                     /locus_tag="ACHJWB_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316730.1"
                     /translation="ENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVR
                     VYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYS
                     GVIDMPAGHGHMTLEFSVSAWLVNNWYPTA"
BASE COUNT           95 a           93 c          111 g           96 t
ORIGIN      
        1 gagaactgtc gggttctggg aaaactgtcc gcgaaccaga ttgaaggcga tctcgttaaa
       61 acagtgggca aagctttccc ccgggattcc cgtgcaccgg agcggtggcc atcagggacc
      121 attaccgtca gggtttatga cgatcagccg tttgaccggc aaattgttat tccggcggtg
      181 gcattcagcg gcgctaaaca tgagagagag catactgata tttactcctc atgccgtctg
      241 atagtgcgga aaaacggtgc tgaaatttat aaccgtaccg cgctggataa tacgctgatt
      301 tacagtggcg ttattgatat gcctgccggt cacggtcaca tgacgctgga gttttcggtg
      361 tcagcatggc tggtaaataa ctggtatccc acagc
//
LOCUS       DAZFAH010000601          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFAH010000601 DAZFAH010000000
VERSION     DAZFAH010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.604"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..>392
                     /locus_tag="ACHJWB_005327"
     CDS             3..>392
                     /locus_tag="ACHJWB_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO4316731.1"
                     /translation="MEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAESWEDIEDF
                     GETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAI
                     DGKTLRHSYDKSRRRGAIHVISAFSTMH"
BASE COUNT          111 a           71 c           88 g          122 t
ORIGIN      
        1 tgatggaaca tatttctatt atccccgatt acagacaagc ctggaaagtg gaacataaat
       61 tatcggatat tctattgttg actatttgtg ccgttatttc tggtgcagaa agttgggaag
      121 atatagagga tttcggggaa acacatctcg atttcttgaa gcaatatggt gattttgaaa
      181 atggtattcc tgttcacgat actattgcca gagttgtatc ctgtatcagt cctgcaaaat
      241 ttcatgagtg ctttattaac tggatgcgtg actgccattc ttcagatgat aaagacgtca
      301 ttgcaattga tggaaaaacg ctccggcact cttatgacaa gagtcgccgc aggggagcga
      361 ttcatgtcat tagtgcgttc tcaacaatgc ac
//
LOCUS       DAZFAH010000602          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFAH010000602 DAZFAH010000000
VERSION     DAZFAH010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.605"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..90
                     /locus_tag="ACHJWB_005328"
     CDS             <1..90
                     /locus_tag="ACHJWB_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4316732.1"
                     /translation="RPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            267..>390
                     /locus_tag="ACHJWB_005329"
     CDS             267..>390
                     /locus_tag="ACHJWB_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HFO4316733.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDG"
BASE COUNT          103 a          101 c           94 g           92 t
ORIGIN      
        1 cgtccgtata tgggctttga tcaggtggca gagcaggaga tcatggacga aatcagaaaa
       61 cgctttaaaa aggccacaga aacgccttaa ttgtttaagg tatgcaaatg cattacctta
      121 cccccttcag gcgcgtgtcg tgattttcta acctgaattt aacgggcttt aaaatctgcg
      181 tgaagcgcct ttgtattttt cccctgaaag cacttcctga aatcccgcaa aacccgccct
      241 gaaaattccc ggcctatgct gccggaatga agacgaaaaa cacgcccaaa cttgcttatg
      301 ccattctgaa tgccatcagc ctgtccgcag acggggacgg tgactggtgt cagatcatgc
      361 cagccggtcg ggtaaaggcc cgtgacggtc
//
LOCUS       DAZFAH010000603          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFAH010000603 DAZFAH010000000
VERSION     DAZFAH010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.606"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>387
                     /locus_tag="ACHJWB_005330"
     CDS             <1..>387
                     /locus_tag="ACHJWB_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4316734.1"
                     /translation="GEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAK
                     TCEELAGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTE
                     SDGKAFPQPAGAALPVREAATQEEIKT"
BASE COUNT          107 a           85 c          123 g           72 t
ORIGIN      
        1 ggcgaaggtg aatcccgggt aaccgaagag cggccagtaa aatctgaagc acaggatatg
       61 ctgaccgggg aggtcgaaca aaaagttacc gcagacatga tgattgagtt tatcggtcag
      121 gatggggcta aaacgtgtga ggaactggcg ggtaagttcg gtgtcagtac tcgcaaggtt
      181 gcttccacgc tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt
      241 aaatttcgtt actgcatgcc gggcgataat ttaccagcag agccgaaagc cgcgctggta
      301 acggaaagtg atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa
      361 gccgcaacac aggaagaaat taaaaca
//
LOCUS       DAZFAH010000604          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFAH010000604 DAZFAH010000000
VERSION     DAZFAH010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.607"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>386
                     /locus_tag="ACHJWB_005331"
     CDS             <1..>386
                     /locus_tag="ACHJWB_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316735.1"
                     /translation="VEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQ
                     PVWDTETTQLFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFP
                     RSGSGRWERIPRELPTGRPAMLHIFEP"
BASE COUNT           77 a           92 c          132 g           85 t
ORIGIN      
        1 gtggaggcgg cctggtcgga atacgccgaa gggatgtctg gcgagatcga cgtggaagga
       61 aaacgcacgt tcacggaatt tatccgtgaa ggtgtgggcg ttcatgcgtt taacggcgaa
      121 atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac gcgttttaaa
      181 gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaaccg ttttctgcgg
      241 gccggggtgg aggtcgatcg atatggccgt gccgttgcgt accatatctg tgaggatgat
      301 tttcctcgct ccgggagtgg acgatgggaa cggatcccgc gtgaacttcc caccgggcgt
      361 ccggccatgc tgcatatttt cgagcc
//
LOCUS       DAZFAH010000605          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFAH010000605 DAZFAH010000000
VERSION     DAZFAH010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.608"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..350
                     /locus_tag="ACHJWB_005332"
     CDS             144..350
                     /locus_tag="ACHJWB_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4316736.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          114 a           77 c           91 g          103 t
ORIGIN      
        1 aatgtaaata aaccatcaat aagcaaaaat aagtggtcgg atgagtagta aaattacaca
       61 aaaggcggta ctattttcat cagataaaca aattcatttt tccggtgcca ttcaccgggt
      121 agatacaaaa gggggagaaa agtatggaac tctacagaga atatcctgca tggcttatct
      181 ttttacgccg tacttatgcg gttgcagcgg gtgttctggc gctgcctttc atgcttttct
      241 ggaaagaccg cgcccgcttt tacagctacc tgcatcgcgt ctggtcgaaa accagcgata
      301 aaccggtgtg gatggatcag gccgaaaagg caactggtga tttttattga ttgctgagtt
      361 ttgaatacat tgaaaaccgc cagcg
//
LOCUS       DAZFAH010000606          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFAH010000606 DAZFAH010000000
VERSION     DAZFAH010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.609"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>369
                     /locus_tag="ACHJWB_005333"
     CDS             <1..>369
                     /locus_tag="ACHJWB_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4316737.1"
                     /translation="GDLALAEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDE
                     LALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTR
                     SAGEREEEQAREALLALEAEL"
BASE COUNT          100 a           82 c          123 g           64 t
ORIGIN      
        1 ggggatctgg cgctggctga atttattcag tcagcctggg aaacgacaaa gaatgtgccc
       61 ggcgttggtg cgttgtttgg tgaactggca gaagagaacc gcgtatttat tgagaaaaca
      121 aaacgcgatg aactggcgct gagaaaatcc attgcggaac gggatgcgcg tatacgccag
      181 ggggaaatgg ggtacatcaa ccgctcgcgt gcaacaggcg tcagcaaagg tcctgggcag
      241 caggaagccg tcagccgtct ggctgaagag ctgacaggta aaaagcatac atcaccgaaa
      301 acgcgctctg ccggggagag ggaagaggag caggcaagag aggctctgct tgcccttgaa
      361 gctgagctc
//
LOCUS       DAZFAH010000607          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFAH010000607 DAZFAH010000000
VERSION     DAZFAH010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.610"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /locus_tag="ACHJWB_005334"
     CDS             <1..276
                     /locus_tag="ACHJWB_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4316738.1"
                     /translation="PRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRA
                     FRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT           89 a           82 c          129 g           64 t
ORIGIN      
        1 ccacggcgtt atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga
       61 catgtgtcgc ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag
      121 tggggacggg cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc
      181 ggcgcgatga tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg
      241 ttcgatgcgt cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa
      301 cagcgcctga cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa
      361 actt
//
LOCUS       DAZFAH010000608          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFAH010000608 DAZFAH010000000
VERSION     DAZFAH010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.611"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          127 a           56 c           87 g           93 t
ORIGIN      
        1 gaagaagaaa aatatcaagg tgatatagcc tatatgcctt tgatgtggag gaatgaatgt
       61 gatgggagtg atgtatctga atagttgaaa aaccgcagac acaccttatg caagaacgtg
      121 ctgcggttgg ctggtaaatt tttcgatagt gcgagtattg aatgatttcc agccgttatt
      181 gattttacgc gtaaatccat gaaaaaacta cttatctgtt ggggagtttt ttggggcata
      241 tatgggacag aaataggccc cagatagaca ctgagatcga acttaggatg cttttaaaaa
      301 aatgcaacta tctgaaaaaa cctagaaaac gccaaggaaa ccacaggatg ggaaaaaaca
      361 cct
//
LOCUS       DAZFAH010000609          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFAH010000609 DAZFAH010000000
VERSION     DAZFAH010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.612"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>355)
                     /locus_tag="ACHJWB_005335"
     CDS             complement(<1..>355)
                     /locus_tag="ACHJWB_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO4316739.1"
                     /translation="TTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGSTGDS"
BASE COUNT           80 a          109 c           91 g           75 t
ORIGIN      
        1 acgagtcgcc tgtacttcct gttgatgcca gtaatccggc ttccttaagc cgctgtgtgt
       61 aggccagcga tacatactga gaacctttat cactgtgatg gaccgtgccg gacggtcgac
      121 gggcccataa cgcctgctcc agtgcatcca gcacgaatgt cgtctccatg gacgatgaga
      181 cccgccaccc cacaatgtat ccggcaaaca catcaatgat gaacgccaca tagacgaagc
      241 cctgccatgt gctgacgtaa gtaaaatcag ccacccacag ctggtcaggt cgttctgcca
      301 cgaactgacg gtttacgcgg tcgcctgcgg caacggcttt ccggctgatg gtcgt
//
LOCUS       DAZFAH010000610          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFAH010000610 DAZFAH010000000
VERSION     DAZFAH010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.613"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..263
                     /locus_tag="ACHJWB_005336"
                     /pseudo
     CDS             1..263
                     /locus_tag="ACHJWB_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspO"
BASE COUNT          143 a           90 c           59 g           61 t
ORIGIN      
        1 caaaaacaga ttttcaagtt cacaagtaca ttacccggaa atatccggtc ccataaaaga
       61 caagccagcg tcaaagaact gcatacttac atcaacaaca tgtaatgtag atagctatac
      121 agtgtaccaa aaaaaaagcc tgtggttttg acatgcgccc acccggcgca ggagaaagaa
      181 ccccaaaact aaaactctca gttactgaga tgacatggct atctaaaact atagaaacag
      241 agatacacaa cacaaaagaa tagcaaccac ttaccagaga acacaaaaag ccacagaccc
      301 gaaacacctg actgcaagca accacctcca caggaggtgg ttttaccatg aac
//
LOCUS       DAZFAH010000611          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFAH010000611 DAZFAH010000000
VERSION     DAZFAH010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.614"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>353
                     /locus_tag="ACHJWB_005337"
     CDS             <1..>353
                     /locus_tag="ACHJWB_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316740.1"
                     /translation="AYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYP
                     DEHTVIRCILPPEDERDRHPGESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGRE
                     VLRHSNRGFACEQGW"
BASE COUNT           84 a          109 c          107 g           53 t
ORIGIN      
        1 gcctatgaca aatccggcag gctgacgggt atcttcagcc cggacggcac atcacagcgc
       61 accggctacg acgaacgcgg gcgggtgaat gtcaccactc agggccgacg ggccattgaa
      121 taccactacc ccgacgaaca caccgtcatc cgctgtatcc tgccaccgga agatgaacgc
      181 gacagacacc ccggtgaatc cctgctgaaa accacgtacc gctacaacgc cgccggagaa
      241 ctgacggagg tcattctgcc gggggatgag acgctgacgt tcagccgtga tgaggcggga
      301 cgtgaagtgc tccggcacag taaccggggt tttgcctgtg aacagggctg gaa
//
LOCUS       DAZFAH010000612          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFAH010000612 DAZFAH010000000
VERSION     DAZFAH010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.615"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..212
                     /locus_tag="ACHJWB_005338"
     CDS             <1..212
                     /locus_tag="ACHJWB_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316741.1"
                     /translation="KMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSR
                     EEETTRPTLKELAAKLSGGVNGYSR"
     gene            196..>347
                     /locus_tag="ACHJWB_005339"
     CDS             196..>347
                     /locus_tag="ACHJWB_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4316742.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDA"
BASE COUNT           85 a           79 c          112 g           71 t
ORIGIN      
        1 gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa cgaagcgctg gactgcctgg
       61 tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg gcagcttgat ctggctgtac
      121 tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct gaaagaactg gcagcgaagc
      181 tgtccggagg agtgaatggt tacagtcgct gaactgcagg cgctgcgtca ggcgcgcctt
      241 gatttattaa ccggtaaacg ggtggtgtct gtccagaaag atggtcgcag aattgaatat
      301 acggcggctt ctctggatga gcttaaccgg gcgatcaatg atgcgga
//
LOCUS       DAZFAH010000613          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFAH010000613 DAZFAH010000000
VERSION     DAZFAH010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.616"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>347
                     /locus_tag="ACHJWB_005340"
     CDS             <1..>347
                     /locus_tag="ACHJWB_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4316743.1"
                     /translation="SVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQGSQFLK
                     LTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHLPGFRH
                     IQTQQATLQAQRMP"
BASE COUNT           89 a           95 c           77 g           86 t
ORIGIN      
        1 tccgtttttg aatatgacct cttggggagt ggaaaagcgg cttcagttgg cgcaaaaccg
       61 gtgcctccac aggtctttaa ttatttagaa gcgcttagcc tggcaagtaa tcaagggagc
      121 cagttcctca aactcacctc tcgctcaggc ttcaaattgc ttcaggttca aaactacgca
      181 gggatgcttt caacacctca cggtttccag cttgagatac tgcccaaggt tggtaaaaac
      241 ctcacagcgg ctaatgcacg tcaaacattg ctgacgatgc taagccatct gcctgggttc
      301 cgacatattc aaacccagca agccactctt caggctcagc gtatgcc
//
LOCUS       DAZFAH010000614          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFAH010000614 DAZFAH010000000
VERSION     DAZFAH010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.617"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..>333
                     /locus_tag="ACHJWB_005341"
     CDS             7..>333
                     /locus_tag="ACHJWB_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO4316744.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVS"
BASE COUNT          104 a           67 c           54 g          108 t
ORIGIN      
        1 gggcatatgc aagtcttttc cagtgatgtc tacttcactg taggcactaa tgcattatta
       61 gcatcccaaa aagaatatta cagcgattta gttgcgttgg ttgatcttgg acattcgttc
      121 gttgttattg atgagcatca acatcgaaat ctcaaaccaa atacagaacc tgtaaacatc
      181 cttttgagca acaatttcat caggatcaat aagaatatta cattatctga tttaacgcat
      241 ttcctggttt caaatcttca tacacaaaat gtgtattcca cacaagaacc acttacccat
      301 gatgagatag atatcctgcg gttatgtgtt tct
//
LOCUS       DAZFAH010000615          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFAH010000615 DAZFAH010000000
VERSION     DAZFAH010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.618"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..299)
                     /locus_tag="ACHJWB_005342"
     CDS             complement(<1..299)
                     /locus_tag="ACHJWB_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316745.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETA"
BASE COUNT           70 a          118 c           82 g           58 t
ORIGIN      
        1 gcggtctccc gcatccccag ccagcgccag ttcggacggt agctgatcag aaacatatgc
       61 ccgacaatgt gatccttatg cagagccacc gcattagccg ctattccgtt attgcgcacc
      121 agatcatctg cccgggcatt ccccagacgc aacgcgggca gcagggccgc atcggcactc
      181 tgcgccggtg gcaaccactc agccatttgc ccgccaaatc ctgcaccgcc cccgttgtag
      241 ctgagactct cacgaagcgg aacgccgttc acatcaatca ggacaggcgt tcgtttcata
      301 acctcactcc cagcggacga cggcgacg
//
LOCUS       DAZFAH010000616          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFAH010000616 DAZFAH010000000
VERSION     DAZFAH010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.619"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..299)
                     /locus_tag="ACHJWB_005343"
     CDS             complement(<1..299)
                     /locus_tag="ACHJWB_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4316746.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETA"
BASE COUNT           67 a          118 c           83 g           60 t
ORIGIN      
        1 gcggtctccc gcatccccag ccagcgccag ttcggacggt agctgatcag aaacatatgc
       61 ccgacaatgt gatccttatg cagtgccacc gcattggccg ctatcccgtt attgcgtacc
      121 agatcatctg cacgggcatt ccccagacgc aacgcgggca acagagctgc atcggcactc
      181 tgcgccggtg gcaaccactc cgccatttgc ccgccaaatc ctgcgccgcc cccgttgtag
      241 ctgaggctct cccgaagcgg aacgccgttc acatcaatca ggacaggcgt tcgtttcata
      301 acctcactcc cagcggacga cggcgacg
//
LOCUS       DAZFAH010000617          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFAH010000617 DAZFAH010000000
VERSION     DAZFAH010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.620"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>326
                     /locus_tag="ACHJWB_005344"
     CDS             <1..>326
                     /locus_tag="ACHJWB_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316747.1"
                     /translation="TFHIWTAYSPFTTWIQIVYDWLDALKDPNGVKTFVNTTLGETWE
                     EAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVD
                     KIIIMGR"
BASE COUNT           76 a           73 c          106 g           71 t
ORIGIN      
        1 acgttccata tctggacggc gtacagtccg ttcaccacct ggatacagat agtctatgac
       61 tggctggatg cactgaaaga tcccaacggc gtgaaaacct ttgtgaacac cacgctgggc
      121 gagacatggg aagaggccgt gggcgaaaaa ctcgatcacc aggtactgat ggataaggtg
      181 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
      241 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      301 gtggataaaa tcatcattat ggggcg
//
LOCUS       DAZFAH010000618          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFAH010000618 DAZFAH010000000
VERSION     DAZFAH010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.621"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>321
                     /locus_tag="ACHJWB_005345"
     CDS             <1..>321
                     /locus_tag="ACHJWB_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316748.1"
                     /translation="ISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITM
                     PKTHNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQ
                     LVAEE"
BASE COUNT           81 a           79 c           93 g           68 t
ORIGIN      
        1 atttcccgtg tctgctggga caccgggggg atcgatggcg aaatcgttta tcagagatca
       61 aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg catctgtcta tggcaagccg
      121 gtgatcacca tgccaaaaac ccacaatcag cggggcgtgt atctgtgtga agtgggaacg
      181 gacaccgcaa aagaaattct ctatgcccgt atgaaagccg atcccacgcc tgcggatgaa
      241 gccacgtcgt atgccatccg ttttcctgat gatccggaga ttttttcgca gacagaggcg
      301 cagcaactgg tggcggaaga g
//
LOCUS       DAZFAH010000619          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFAH010000619 DAZFAH010000000
VERSION     DAZFAH010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.622"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>319
                     /locus_tag="ACHJWB_005346"
     CDS             <1..>319
                     /locus_tag="ACHJWB_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4316749.1"
                     /translation="QIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDK
                     VVRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLL
                     RVDA"
BASE COUNT           74 a           68 c          105 g           72 t
ORIGIN      
        1 tacagattgt ctatgactgg ctggatgcac tgaaagatcc caacggcctg aaaacctttg
       61 tgaacaccac gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg
      121 tactgatgga taaggttgtg cgttacacgg ctgcggtgcc ttcccgggtg gtttatctga
      181 cggcgggcat tgactcgcag cgaaaccgtt ttgagatgta tgtctgggga tgggcaccgg
      241 gagaggaagc ctttctggtg gataaaatca tcattatggg gcgtcccgat gaggaagaga
      301 cgctgttacg tgtggatgc
//
LOCUS       DAZFAH010000620          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFAH010000620 DAZFAH010000000
VERSION     DAZFAH010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.623"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>316
                     /locus_tag="ACHJWB_005347"
     CDS             <1..>316
                     /locus_tag="ACHJWB_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4316750.1"
                     /translation="GNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIR"
BASE COUNT           94 a           77 c           88 g           57 t
ORIGIN      
        1 ggggaaacca tattgtcgac agagtccgtc tgaaaaacgc acggatcctc ggtgataata
       61 atgccagaaa tggagcagac agactggtca gcggaacaga aatccagacg aaatattgtt
      121 caactgcagc ccgtagcgtc ggtgcggcat tcgacggaca aaacggacag tatcgttaca
      181 tgggaaataa tggccccatg caactggaag tcccccgtga tcagtatgcc ggcgctgttg
      241 aaaccatgag gaataagatc cgcgaaggta aagtacccgg tgtaaccgat cccgcagaag
      301 cgtcccggct gattcg
//
LOCUS       DAZFAH010000621          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFAH010000621 DAZFAH010000000
VERSION     DAZFAH010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.denovo.624"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHJWB_005348"
     CDS             <1..>314
                     /locus_tag="ACHJWB_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4316751.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFAH010000622        11773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.guided.144, whole genome shotgun sequence.
ACCESSION   DAZFAH010000622 DAZFAH010000000
VERSION     DAZFAH010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.guided.144"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..832
                     /locus_tag="ACHJWB_005349"
     CDS             59..832
                     /locus_tag="ACHJWB_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4316752.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(889..1245)
                     /locus_tag="ACHJWB_005350"
     CDS             complement(889..1245)
                     /locus_tag="ACHJWB_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4316753.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1238..1516)
                     /locus_tag="ACHJWB_005351"
     CDS             complement(1238..1516)
                     /locus_tag="ACHJWB_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4316754.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1621..3333)
                     /locus_tag="ACHJWB_005352"
                     /pseudo
     CDS             complement(1621..3333)
                     /locus_tag="ACHJWB_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3305..4090
                     /gene="lafU"
                     /locus_tag="ACHJWB_005353"
     CDS             3305..4090
                     /gene="lafU"
                     /locus_tag="ACHJWB_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4316755.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            4161..5216
                     /gene="dinB"
                     /locus_tag="ACHJWB_005354"
     CDS             4161..5216
                     /gene="dinB"
                     /locus_tag="ACHJWB_005354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4316756.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5213..5665
                     /locus_tag="ACHJWB_005355"
     CDS             5213..5665
                     /locus_tag="ACHJWB_005355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4316757.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            5910..7049
                     /locus_tag="ACHJWB_005356"
     CDS             5910..7049
                     /locus_tag="ACHJWB_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4316758.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            7046..7660
                     /gene="prfH"
                     /locus_tag="ACHJWB_005357"
     CDS             7046..7660
                     /gene="prfH"
                     /locus_tag="ACHJWB_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4316759.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(7717..9174)
                     /gene="pepD"
                     /locus_tag="ACHJWB_005358"
     CDS             complement(7717..9174)
                     /gene="pepD"
                     /locus_tag="ACHJWB_005358"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4316760.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9435..9893
                     /gene="gpt"
                     /locus_tag="ACHJWB_005359"
     CDS             9435..9893
                     /gene="gpt"
                     /locus_tag="ACHJWB_005359"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4316761.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9985..11229
                     /gene="frsA"
                     /locus_tag="ACHJWB_005360"
     CDS             9985..11229
                     /gene="frsA"
                     /locus_tag="ACHJWB_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4316762.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            11287..11688
                     /gene="crl"
                     /locus_tag="ACHJWB_005361"
     CDS             11287..11688
                     /gene="crl"
                     /locus_tag="ACHJWB_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4316763.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         2847 a         2975 c         3089 g         2862 t
ORIGIN      
        1 ttataataat ggtttattaa gtttgttcag gtacgggccg ggatacactg caacatcaat
       61 gcatgatcag ccattattga gtatgtcctt gccgtcgatt ccctcttttg tgttgtcggg
      121 attactgttg ctttgcttgc cgttttcttc atttgccagc gccaccacat cacatatcac
      181 tttcagctac gccgcccgcc agcggatgca aaatcgtgcg cgtttattaa aacagtacca
      241 aacccatctg aaaaaacagg ccagctatat tgtggaaggc aatgccgaaa gcagaagggc
      301 gctacgccag cacaaccgcg aacagataaa acagcatcca gaatggtttc ctgcgccgct
      361 caaggcgagt gacagacgct ggcaggcgct ggcggaaaac aaccatttct taagcagcga
      421 ccatctgcat aacatcaccg aagtggcgat tcaccgtctg gagcaacagc ttggcaagcc
      481 ttacgtctgg ggcggtacgc ggcccgatca aggcttcgac tgtagcgggt tagtttttta
      541 tgcctacaac aagatccttg aggcgaaact cccacgcacg gctaatgaga tgtatcacta
      601 tcgccgggca accattgtgg cgaataacga cctgcgccgg ggagatttgc tatttttcca
      661 tatccacagc cgcgagattg ccgaccatat gggggtgtat ctgggcgacg gtcagtttat
      721 tgagtcaccg cgcaccgggg aaaccattcg ggtaagtcgg ttagctgaac ctttctggca
      781 ggatcacttt ttaggcgcgc ggcggatttt gacggaggag acgattttgt aaatgggtaa
      841 tattgacgaa ctggcaaaag gtcggatgcc acgcggcatc cgacctgttt acatctgggc
      901 tgaaaactca cgttctaaga catcaacaat gagctggttt tttgaaatgg aatgttgtct
      961 ggcgacgatg gtcagccgcg aatcaatgtg atgaggaaca cgaagcgtca ggcgtttttg
     1021 ctcttcttct tcacgaaaag gggcaatacc gtcctcttca caatccgcca tccactcatc
     1081 cagcgcgctt tcgccttcac gatataatgc ctcaatactt gtggctacaa agtcacaata
     1141 accgtttaca ttgaggaact tgccgcgaaa tgcgttgata tcggcttcaa atgtcactgc
     1201 ggctggttga cctttaattg tgataatgtt cgatgcttta ttcataatct acccctacac
     1261 agttaacaaa ccactcctta agacctgcta ctgcgccttt atccatcaca ttctttggat
     1321 gtggctcatg tgtgctgaat ttggtcgttc caatctgaaa ttttcgtctg gagccgttgt
     1381 tattttttat tgttccaccc acagctttaa tgagtgcaac aacatcatcc catttaattc
     1441 cagattttgg cggctccgtc attacatcgt cgaatgtttt tctatgacga gaacctagcg
     1501 cgctgacctt aatcatacta tcaatccatg acgtcattgt atggtgtcat cgtagtctct
     1561 ttgcagtgtg acgtcaatta atggtgtcag gtgctgcaaa gcatggaaaa gaatccccca
     1621 tcaactcaac gcccccataa tcttcaactc cagctcatcc ggcacttcgt tatacgacag
     1681 cacatgcagc cccggcgcaa acaaccttgc ataacgcgcc agcaaagggc gcagctgcgg
     1741 tggcaccagc agcaccgggt ctttccccgc cgctttcatc tgctctttca cctgtggcat
     1801 cgtactctgg aactggttga gcatattcgg gtccactggc acgctgtcga gcatcacttt
     1861 accgccctgt tgcgcctgat tcactaggtt agtcagcaga ttttccagct cattattcag
     1921 ggtatacacc gtcagctcct gcttgcgaac gaacggatgg gtaatgctgc gccgcagcgc
     1981 caggcgcaca tcggctgcca gcagaatatg atctttggtc accgcgctac tggcgaccag
     2041 cacggtggcg atggtgacga tatcgcgcag ggaaacgcct tcggtcagca gcgcccggta
     2101 cactttcagc aactggctgt aattgagcac cgcgcttaaa tcctccgcca ggcgcggtgc
     2161 catcgacgcc aaacggttat gcaactgcgt aatatcgtca tagttaaata aatcaggaat
     2221 atagctgcgc acaatcttat ttacatgggt agcaatcacg ctggcgctgt cgatcacctg
     2281 ataccccata ttcagcgcct tcgctttctg cgcaggctgg atccaggtca ccggcatccc
     2341 gtacgccggg tcgttcccca gcacgccgtc tatctcgccg taggtttcgc tggagggcag
     2401 cgccatcagt ttatccgccg gaatatccgc ttcatcagcc ttaatgccgt tgatgaaaat
     2461 agcgtactgg ctgggcttaa ggcggaagtt ttcccgaatg cggatctccg gcagcagcac
     2521 gccgttgccg tcggatatca cctgtcgcac gccgcgaata cgctgggtga gcgggttgcc
     2581 ctgggctttg tccaccagcg ccaccagctt gtagccgagg cttaagctga tgggctcgat
     2641 cagcggaatg gtttcccaac tgacctgttg ctcgctggtt tcggtgatgg tgcgggtcag
     2701 cgtttcgaga cttttctctt ccgcttccgc cgcctgcggg cgtttgctca tccgccagcc
     2761 ggtaaagcca agcagggcgc tgaacagcaa aaacggcaga tgtggcattc caggcactac
     2821 cgccagtacg aacataattc cggtagcggt gtagagcacc gatgggcttg ccagcaactg
     2881 gttacgcacg tcatgggcga tatcgccgtt gtcgctgacg cgggtgacga taatcgccgc
     2941 cgcggtggag agtagcaggg aagggatttg cgccaccagc ccgtcgccga tggtcatcag
     3001 cacatactgc tggaaggcgg cgtcggcgct caggttgtat ttgaagatcc cgatacagac
     3061 gccgccgatc aggttgatcg ccagaatcat catcccggcg atagcatccc cgcgcacaaa
     3121 tttcgatgcc ccgtccatcg cgccgtagaa atcggcctcg ctggcaacat ctttacgtcg
     3181 ggtttgtgcc tgcgcctgat tgatcaaccc ggcattaaga tcggcgtcaa tcgccatctg
     3241 cttaccgggc atcgcgtcca gggtaaagcg ggcagaaacc tcggagatac gctcggcccc
     3301 tttggtgacg acaataaagt tgattgtcaa cagcgtcagt aaatccgagc gtgaaagcat
     3361 tatcgccgcg ctgcacgggc agtcgatttt taacggtggt gggctgtcgc cgttaaataa
     3421 aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag agacggagaa
     3481 aaaagcgcgc gacgtcaacg agaaaacagc gctgctgaag aagaaatccg ccaccgagct
     3541 tggcgagctg gcaaccagta tcaacaccat tgcccgtgat gcgcatatgg aagcgaatct
     3601 ggagatggag attgtcccgc agggattacg cgtgctgatt aaagacgacc agaatcgcaa
     3661 tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaacgctgc tggtggagct
     3721 cgcgccagtg ttcgactcgc tcgataataa aattattatt accgggcata ccgatgcgat
     3781 ggcctacaaa aacaatatct acaacaactg gaacctttcg ggcgatcgcg cgctttcagc
     3841 tcgtcgggtg ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg taagcgcaat
     3901 ggcggaccag atgctgctgg acgccaaaaa tccgcaaagc gcgggcaacc ggcgcattga
     3961 gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg
     4021 ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg
     4081 aatgcgctaa atgctgaatc tttacgcatt tctcaaaccc tgaaatcact gtatacttta
     4141 ccagtgttga gaggtgagca atgcgtaaaa tcattcatgt ggatatggac tgctttttcg
     4201 ccgcagtaga gatgcgcgac aatcccgccc tgcgcgatat ccctattgct attggcggca
     4261 gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac
     4321 gtagcgcaat gccgacaggg atggcgctca aattatgccc acatctcacc ttgcttccgg
     4381 ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca
     4441 cctcgcgcat tgagccgttg tcactggatg aggcttatct cgatgtcacc gatagcgtcc
     4501 attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt ttcagcgagc
     4561 tgcaactgac ggcgtctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg
     4621 acatgaataa acccaacggc cagtttgtga ttacgccggc agaagttccg gcatttttac
     4681 aaaccttacc actggcaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag
     4741 cgatggggct gcggacctgc ggtgatgtac aaaagtgtga tctggtgata ctgcttaaac
     4801 gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg
     4861 ttaacagcga acggttgcga aaatccgtcg gcgtggaacg cacgatggcg gaagatatcc
     4921 accactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc
     4981 tggcaaaggt aaaacctgat ttactgattg cccgccaggg ggtgaaatta aagttcgacg
     5041 attttcagca aaccactcag gagcacgtct ggccgcggct gaataaagct gacttaatcg
     5101 ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc
     5161 tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgaataa
     5221 catacaaata agagactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
     5281 agcggttacg atgactgcca gtcagcatta ttcaccacaa caaattgccg cctgggcgca
     5341 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
     5401 taatgcaaaa ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt
     5461 tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt tgattaagtc
     5521 tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg
     5581 ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta ttaattttta
     5641 tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc aagcctagta
     5701 tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
     5761 atgactcttt gcagaccttt ccaggattaa ttcttttttc ttgccctgga ttcgtctgcc
     5821 atttcctgat tttttaattg ttactctcaa taattcaagt cgcaacacat gcaacttgaa
     5881 gtatgacgag tctatttacg gaatggtata tgggcaatta tattcgtccc ttatccgatg
     5941 cggtatttac catcgcatct gatgacctgt ggatcgagag tttagcgatc caacaattac
     6001 acaccacggc aaatttaccc aacatgcagc gcgtagtggg gatgccagat ttacatcccg
     6061 gacgcggcta tccgattggc gcagcgttct tctctgttgg tcgtttttat ccggcactgg
     6121 tcggcaatga tatcggctgc ggtatggcgc tatggcaaac agatattctc gctcgcaaat
     6181 acaacgccga taagtttgaa aagcgattat cagatctgga tgacgttgct gaagaaagct
     6241 ggctggagga aaacctgccg tcagcatttg cacagcatcc gtggcgcagc tcgcttggct
     6301 ctatcggtgg cggcaatcat ttcgctgaac tgcaacaagt tgatcaaatc atcaacgctg
     6361 aactgtttgc actggcaggt ctggatgcac aacatctgca actgttggtt catagcggct
     6421 cgcggggggt ggggcagtct attttacagc ggcatattgc ctcgttctcg catcatggtt
     6481 tgcctcaagg cagtgacgac gcgctacggt atattgcgga acatgacgat gcgctggcgt
     6541 ttgcgtgtat taatcgccag atgatcgctt tgcgcataat gcaacaagtt aaggccacgg
     6601 gaagtccggt tctggatgtg gcgcataact ttgttagcgc gtgtcaaatc ggcgatcaac
     6661 agggctggtt gcatcgtaaa ggtgccacgc cggatgacaa cggtctggtg attattcccg
     6721 gttcacgtgg tgattactcc tggattgtac agcctgttgc gaacgaaaaa acgctccact
     6781 cgctggcgca tggggcaggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
     6841 cgaaatacac ggcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     6901 ataaacaact catctttgaa gaagcgccac aagcctataa atcggctgaa agcgtggtgc
     6961 aatgtctggt gcaggccggt ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc
     7021 tcaaaaacag tggagggaaa aaaggatgat cttgctacaa ctctcctcag cgcaggggcc
     7081 ggaagaatgt tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg
     7141 acaagacgtc gcggtaacgg tgttggaaac agaaacgggc cgctactctg acacgctgcg
     7201 ttcggcgctg atttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg
     7261 cactattcag tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt
     7321 tctgggcatt gggcgtttta ccgctgatga gcaggaacaa tcggatgcaa tccgttatga
     7381 gacgttgcgt tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg
     7441 cgccacgcat ttggcatccg gtattagcgt gaaggttcag tcagagcgta gtcagcatgc
     7501 taacaagcgg ctggcacgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa
     7561 tagcgcggtg ctgaaatcgc agcggcgaat gttccatcac cagattgaac gtggcaaccc
     7621 gagacggaca tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga
     7681 aggcgattaa acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag
     7741 cagttcagtc agcagcgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg
     7801 agagtgtgga ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt
     7861 tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggtg ttttgttgaa
     7921 cagacgctga taggtttcac gtacaagatg catcaccgga gaattagcgt ccggctgcca
     7981 gccaggatat gcgcctttcg cttcggtttt cgcgccagct agtttaccca gcgaatccag
     8041 catgctcacc acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc
     8101 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
     8161 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
     8221 atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc
     8281 cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag
     8341 agatttcagg acgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
     8401 gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcagatcca gttcttccgc
     8461 atgacccgcc aggaagcgca ccagtagttt gttggcatta cccaggccaa cgtggatttc
     8521 accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc
     8581 tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc
     8641 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
     8701 gttgctctgt aagccaaaag caccgtccat accggcttct tcggtcatgg tcagcagcac
     8761 ttccagcgga ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc
     8821 aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
     8881 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
     8941 atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt
     9001 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
     9061 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
     9121 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
     9181 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
     9241 tcaacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9301 gaggcagtgt gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9361 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9421 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9481 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9541 gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     9601 ataccgtttg tatttccagc tacgatcacg acaaccagcg agagcttaaa gtgctgaaac
     9661 gcgcagaagg cgatggcgaa ggcttcatag ttattgatga cctggtggat accggtggta
     9721 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     9781 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     9841 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     9901 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     9961 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
    10021 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
    10081 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
    10141 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
    10201 attgtgatga gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac
    10261 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
    10321 tgtgcggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
    10381 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
    10441 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
    10501 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
    10561 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
    10621 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
    10681 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
    10741 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
    10801 cgtttcggcg ctaacgttgc cgtgcgtctg gcatatcttg aatcgccgcg tctgaaagcg
    10861 gttgcctgtc ttggtccggt agttcatacc ctattgagtg attttaagtg ccagcaacag
    10921 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
    10981 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctgggacgt
    11041 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
    11101 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
    11161 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
    11221 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
    11281 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
    11341 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
    11401 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg
    11461 gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa
    11521 gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac
    11581 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
    11641 gaaccggcgg atgattttcg tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc
    11701 ggatgcatca catccggcaa tatctattaa aactgatacg tcatcccaat cgcgacaata
    11761 tcatcattat taa
//
LOCUS       DAZFAH010000623         9645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.guided.166, whole genome shotgun sequence.
ACCESSION   DAZFAH010000623 DAZFAH010000000
VERSION     DAZFAH010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.guided.166"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..364)
                     /locus_tag="ACHJWB_005362"
     CDS             complement(<1..364)
                     /locus_tag="ACHJWB_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4316764.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQ"
     gene            complement(583..1560)
                     /gene="epmA"
                     /locus_tag="ACHJWB_005363"
     CDS             complement(583..1560)
                     /gene="epmA"
                     /locus_tag="ACHJWB_005363"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4316765.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQMLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1885..3693
                     /gene="frdA"
                     /locus_tag="ACHJWB_005364"
     CDS             1885..3693
                     /gene="frdA"
                     /locus_tag="ACHJWB_005364"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4316766.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3686..4420
                     /gene="frdB"
                     /locus_tag="ACHJWB_005365"
     CDS             3686..4420
                     /gene="frdB"
                     /locus_tag="ACHJWB_005365"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4316767.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4431..4826
                     /gene="frdC"
                     /locus_tag="ACHJWB_005366"
     CDS             4431..4826
                     /gene="frdC"
                     /locus_tag="ACHJWB_005366"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4316768.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4837..5196
                     /gene="frdD"
                     /locus_tag="ACHJWB_005367"
     CDS             4837..5196
                     /gene="frdD"
                     /locus_tag="ACHJWB_005367"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4316769.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5259..6392
                     /locus_tag="ACHJWB_005368"
     CDS             5259..6392
                     /locus_tag="ACHJWB_005368"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4316770.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWKPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6481..7014
                     /gene="blc"
                     /locus_tag="ACHJWB_005369"
     CDS             6481..7014
                     /gene="blc"
                     /locus_tag="ACHJWB_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4316771.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVANNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7011..7328)
                     /gene="sugE"
                     /locus_tag="ACHJWB_005370"
     CDS             complement(7011..7328)
                     /gene="sugE"
                     /locus_tag="ACHJWB_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4316772.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7510..7656)
                     /gene="ecnB"
                     /locus_tag="ACHJWB_005371"
     CDS             complement(7510..7656)
                     /gene="ecnB"
                     /locus_tag="ACHJWB_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4316773.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7767..7892)
                     /gene="ecnA"
                     /locus_tag="ACHJWB_005372"
     CDS             complement(7767..7892)
                     /gene="ecnA"
                     /locus_tag="ACHJWB_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4316774.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7944..8510)
                     /gene="efp"
                     /locus_tag="ACHJWB_005373"
     CDS             complement(7944..8510)
                     /gene="efp"
                     /locus_tag="ACHJWB_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4316775.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8552..9580
                     /gene="epmB"
                     /locus_tag="ACHJWB_005374"
     CDS             8552..9580
                     /gene="epmB"
                     /locus_tag="ACHJWB_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4316776.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2487 c         2474 g         2233 t
ORIGIN      
        1 gctgggtcat gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg
       61 tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac
      121 gtggtccgac actattattc atccaggaat agataccgcc ttcttctttg cgataagcag
      181 ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat
      241 aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa
      301 atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag
      361 gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa ctgcactgca
      421 caacattgag caggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata
      481 tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca gaatggcgct
      541 tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct
      601 aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac
      661 acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg
      721 gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg
      781 ttgttgctgc tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt
      841 ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga
      901 tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc
      961 tacgccaaag gtaaacagca attgtagcag cgtgtcgcgg tcttcttcgg tatcagcaac
     1021 attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg
     1081 gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg ctgccgggca
     1141 gtccagcatc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg
     1201 cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca tctcttcatt
     1261 acggaagctg cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgtttcat
     1321 atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggccaac
     1381 gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg
     1441 cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc
     1501 cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat
     1561 agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca
     1621 aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg atctgatggt
     1681 ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc
     1741 gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag
     1801 tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg
     1861 ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc
     1921 gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa
     1981 atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc
     2041 tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg
     2101 ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgcccaacc
     2161 gaaatgaccc aactggaact gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc
     2221 aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc
     2281 ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt
     2341 tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta
     2401 gcaatgaaca tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct
     2461 actggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
     2521 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
     2581 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc
     2641 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
     2701 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
     2761 caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg
     2821 gtttatctcg acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
     2881 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
     2941 ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
     3001 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
     3061 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
     3121 gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc
     3181 gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc
     3241 cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac gccggaactg
     3301 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
     3361 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt
     3421 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     3481 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc
     3541 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     3601 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     3661 gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     3721 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     3781 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     3841 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     3901 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     3961 agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga
     4021 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     4081 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
     4141 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
     4201 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     4261 tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctattgctc
     4321 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     4381 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     4441 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     4501 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     4561 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     4621 tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagct ctgctgcaca
     4681 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
     4741 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
     4801 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     4861 tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     4921 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
     4981 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     5041 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     5101 gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     5161 acagttgtca cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc
     5221 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
     5281 ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
     5341 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
     5401 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     5461 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     5521 tgcttggtgg cgaggctatt gctcgagggg aaatcaagtt aagcgatccc acaacaaaat
     5581 actggcctga acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct
     5641 acactgctgg cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc
     5701 tgcgcttcta tcaaaactgg aagcctgcat gggcgccagg aacacaacgt ctgtatgcca
     5761 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
     5821 aggcgatgca aacacgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
     5881 cgcccgcaga agaaaagaat tacgcctggg gatatcgcga aggcaaggca gttcatgttt
     5941 cgccaggggc gttagatgct gaaacttatg gtgtgaagtc gaccattgaa gatatggcct
     6001 gctgggtacg aagcaatatg aatccccgtg atatcaacga caaaacactt cagcaaggga
     6061 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     6121 aaatgctgga ctggccggta aatcctgaca gcatcattaa cggcagtggc aataaaattg
     6181 cactggcagc acaccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat
     6241 gggtacataa aacaggggca accggcggat ttggtagcta tgtcgcgttt attccagaaa
     6301 aagagctggg tatagtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     6361 ccgcctggca gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc
     6421 ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt
     6481 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt
     6541 tctcctacgc cgccgcgtgg cgtaacagta gcgaataatt tcgacgccaa acgttatctc
     6601 ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc
     6661 accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac
     6721 cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact
     6781 cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggctataa cgttattgca
     6841 ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata
     6901 ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc
     6961 cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg
     7021 ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag gcgcatcggg
     7081 ttagcggact caccgagcag cacaatgccg gtgatagccg cgccgactgc gccaataccc
     7141 gtccacacgg cataagccgt ccctactggt aacgatttca tcgcccaagc aagtagcgcc
     7201 atactgacaa tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg
     7261 gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac taagataatc
     7321 caggacatat caggctccgg agaattgggg ccgtccccgg tgaaaagagg cgtttgcagg
     7381 tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact
     7441 attaccttag tgactatcgc agaaaaaaca aacggcacga catacctgtc gtaccgttat
     7501 tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac cgccatcaga
     7561 aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
     7621 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
     7681 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg acggaaatct
     7741 gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga gttgccgaga
     7801 tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca
     7861 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc
     7921 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
     7981 tccactttga tgacttcgcc gatttgtacg aacagcggaa ccttaaccac agcgccagta
     8041 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
     8101 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
     8161 agagttacga tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt
     8221 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
     8281 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
     8341 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
     8401 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct
     8461 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
     8521 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
     8581 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct
     8641 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
     8701 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
     8761 tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc
     8821 caccgacccg ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa
     8881 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
     8941 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg cacttgagta
     9001 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
     9061 gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
     9121 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt
     9181 tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa
     9241 tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct
     9301 gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgt tggcaaacct
     9361 gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc tagataaagt
     9421 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
     9481 gctgacattg gtgtcgggat atctggtgcc gaaactggcg cgagaaattg gcggcgaacc
     9541 cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagtta
     9601 aacattagcc tgatgcgcta cgcttatcag gccttgtatt atccc
//
LOCUS       DAZFAH010000624         2425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2310F30649
            SAMN43283924-rid23063343.guided.355, whole genome shotgun sequence.
ACCESSION   DAZFAH010000624 DAZFAH010000000
VERSION     DAZFAH010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283924
            Sequence Read Archive: SRR30325181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351443.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:09:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 94 of 212
            Pseudo Genes (incomplete)         :: 108 of 212
            Pseudo Genes (internal stop)      :: 59 of 212
            Pseudo Genes (multiple problems)  :: 43 of 212
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283924-rid23063343.guided.355"
                     /serotype="O 84:H 2"
                     /isolate="2310F30649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..241
                     /locus_tag="ACHJWB_005375"
     CDS             <1..241
                     /locus_tag="ACHJWB_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4316777.1"
                     /translation="DLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKAEGIVEGM
                     LMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            748..1695
                     /gene="stxA1a"
                     /locus_tag="ACHJWB_005376"
     CDS             748..1695
                     /gene="stxA1a"
                     /locus_tag="ACHJWB_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4316778.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1705..1974
                     /gene="stxB1a"
                     /locus_tag="ACHJWB_005377"
     CDS             1705..1974
                     /gene="stxB1a"
                     /locus_tag="ACHJWB_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4316779.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          641 a          440 c          606 g          738 t
ORIGIN      
        1 cgatctgcat gacttgctgg ttgattatta cgtgttgggg gagacgttca tggcgctggc
       61 acggaaacat gggtgctctg acacctgtat aggtaaacgc cttcacaaag cggaggggat
      121 tgttgaaggc atgctgatga tgctgggagt gaggcttgag atggatcggt atgttgagcg
      181 tgaattgccg ggagggagaa cctctgtatt ttatcagcga aaaaatagtt tacgatcgta
      241 aaaatctgca tatcatgata agagtggtta cattgccacg ctgcttaacc cgccgatgcg
      301 cgggtttttt tgtacccaga atcctgtgag ctatacggaa agtacacaga aaggaaggtg
      361 cgaccacaat taataacaaa atcttaaaaa ttgcacatgg cactattagt tttctaaata
      421 ttgtgtattt tttgtattgc aggatgaccc tgtaacgaag tttgcgtaac agcattttgc
      481 tctacgagtt tgccagcctc ccccagtggc tggctttttt atgtccgtaa catcctgtgt
      541 atcaataaat gttgttatct acgtacgtca agtagtcgca tgagatctga ccagatatgt
      601 taaggttgca gctctctttg aatatgatta tcattttcat tacgttattg ttacgtttat
      661 ccggtgcgcc gtaaaacgcc gtccttcagg gcgtggagga tgtcaagaat atagttatcg
      721 tatggtgctc aaggagtatt gtgtaatatg aaaataatta tttttagagt gctaactttt
      781 ttctttgtta tcttttcagt taatgtggtg gcgaaggaat ttaccttaga cttctcgact
      841 gcaaagacgt atgtagattc gctgaatgtc attcgctctg caataggtac tccattacag
      901 actatttcat caggaggtac gtctttactg atgattgata gtggcacagg ggataatttg
      961 tttgcagttg atgtcagagg gatagatcca gaggaagggc ggtttaataa tctacggctt
     1021 attgttgaac gaaataattt atatgtgaca ggatttgtta acaggacaaa taatgttttt
     1081 tatcgctttg ctgatttttc acatgttacc tttccaggta caacagcggt tacattgtct
     1141 ggtgacagta gctataccac gttacagcgt gttgcaggga tcagtcgtac ggggatgcag
     1201 ataaatcgcc attcgttgac tacttcttat ctggatttaa tgtcgcatag tggaacctca
     1261 ctgacgcagt ctgtggcaag agcgatgtta cggtttgtta ctgtgacagc tgaagcttta
     1321 cgttttcggc aaatacagag gggatttcgt acaacactgg atgatctcag tgggcgttct
     1381 tatgtaatga ctgctgaaga tgttgatctt acattgaact ggggaaggtt gagtagcgtc
     1441 ctgcctgact atcatggaca agactctgtt cgtgtaggaa gaatttcttt tggaagcatt
     1501 aatgcaattc tgggaagcgt ggcattaata ctgaattgtc atcatcatgc atcgcgagtt
     1561 gccagaatgg catctgatga gtttccttct atgtgtccgg cagatggaag agtccgtggg
     1621 attacgcaca ataaaatatt gtgggattca tccactctgg gggcaattct gatgcgcaga
     1681 actattagca gttgaggggg taaaatgaaa aaaacattat taatagctgc atcgctttca
     1741 tttttttcag caagtgcgct ggcgacgcct gattgtgtaa ctggaaaggt ggagtataca
     1801 aaatataatg atgacgatac ctttacagtt aaagtgggtg ataaagaatt atttaccaac
     1861 agatggaatc ttcagtctct tcttctcagt gcgcaaatta cggggatgac tgtaaccatt
     1921 aaaactaatg cctgtcataa tggaggggga ttcagcgaag ttatttttcg ttgactcaga
     1981 atagctcagt gaaaatagca ggcggagatt cataaatgtt aaatacatct caattcagtc
     2041 agttgttgcc ggtctgataa tagatgtgtt agaaaatttc tgcatggtga atccccctgt
     2101 gcggaggggc gactggtgaa cggtatgatc tctttgatga tcgtaagcga gaatacgcgg
     2161 gtttggtggc accaggccga actcaccggg aggcacccgg catcatgctg tatacagaga
     2221 ttaggcatat atccaggctc ctcatcgcag gagccttttt acatgcaaaa aaaagcccga
     2281 gtgggttcgg gcaacagcat gagatacttg cattgtcatt tttatcgtgc ggattttaac
     2341 caggattcat aaggctgcgc aactgcgcgg cctttttcgt atttcgggct gtagtccccg
     2401 tgtgtcattc aggcttccgg actac
//
